LOCUS       DBANUU010000001        87922 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000001 DBANUU010000000
VERSION     DBANUU010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 87922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.001"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(175..1650)
                     /gene="ampG"
                     /locus_tag="ACIE68_000001"
     CDS             complement(175..1650)
                     /gene="ampG"
                     /locus_tag="ACIE68_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFX5906383.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(1694..2272)
                     /gene="yajG"
                     /locus_tag="ACIE68_000002"
     CDS             complement(1694..2272)
                     /gene="yajG"
                     /locus_tag="ACIE68_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFX5906384.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2577..2894
                     /gene="bolA"
                     /locus_tag="ACIE68_000003"
     CDS             2577..2894
                     /gene="bolA"
                     /locus_tag="ACIE68_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFX5906385.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3238..4536
                     /gene="tig"
                     /locus_tag="ACIE68_000004"
     CDS             3238..4536
                     /gene="tig"
                     /locus_tag="ACIE68_000004"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFX5906386.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4782..5405
                     /gene="clpP"
                     /locus_tag="ACIE68_000005"
     CDS             4782..5405
                     /gene="clpP"
                     /locus_tag="ACIE68_000005"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFX5906387.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5531..6805
                     /gene="clpX"
                     /locus_tag="ACIE68_000006"
     CDS             5531..6805
                     /gene="clpX"
                     /locus_tag="ACIE68_000006"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFX5906388.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            6993..9347
                     /gene="lon"
                     /locus_tag="ACIE68_000007"
     CDS             6993..9347
                     /gene="lon"
                     /locus_tag="ACIE68_000007"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFX5906389.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9538..9828
                     /gene="hupB"
                     /locus_tag="ACIE68_000008"
     CDS             9538..9828
                     /gene="hupB"
                     /locus_tag="ACIE68_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFX5906390.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            10020..11891
                     /gene="ppiD"
                     /locus_tag="ACIE68_000009"
     CDS             10020..11891
                     /gene="ppiD"
                     /locus_tag="ACIE68_000009"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFX5906391.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQTAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLREEAKIKIGDALEQQ"
     gene            12042..12413
                     /gene="ybaV"
                     /locus_tag="ACIE68_000010"
     CDS             12042..12413
                     /gene="ybaV"
                     /locus_tag="ACIE68_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFX5906392.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            12507..12905
                     /gene="fadM"
                     /locus_tag="ACIE68_000011"
     CDS             12507..12905
                     /gene="fadM"
                     /locus_tag="ACIE68_000011"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFX5906393.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12957..13652)
                     /gene="queC"
                     /locus_tag="ACIE68_000012"
     CDS             complement(12957..13652)
                     /gene="queC"
                     /locus_tag="ACIE68_000012"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFX5906394.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(13717..15417)
                     /gene="ybaE"
                     /locus_tag="ACIE68_000013"
     CDS             complement(13717..15417)
                     /gene="ybaE"
                     /locus_tag="ACIE68_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFX5906395.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15517..16335
                     /gene="cof"
                     /locus_tag="ACIE68_000014"
     CDS             15517..16335
                     /gene="cof"
                     /locus_tag="ACIE68_000014"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFX5906396.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16488..16946
                     /gene="decR"
                     /locus_tag="ACIE68_000015"
     CDS             16488..16946
                     /gene="decR"
                     /locus_tag="ACIE68_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFX5906397.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            16976..18748
                     /gene="mdlA"
                     /locus_tag="ACIE68_000016"
     CDS             16976..18748
                     /gene="mdlA"
                     /locus_tag="ACIE68_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFX5906398.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            18741..20522
                     /gene="mdlB"
                     /locus_tag="ACIE68_000017"
     CDS             18741..20522
                     /gene="mdlB"
                     /locus_tag="ACIE68_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFX5906399.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20703..21041
                     /gene="glnK"
                     /locus_tag="ACIE68_000018"
     CDS             20703..21041
                     /gene="glnK"
                     /locus_tag="ACIE68_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFX5906400.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            21071..22357
                     /gene="amtB"
                     /locus_tag="ACIE68_000019"
     CDS             21071..22357
                     /gene="amtB"
                     /locus_tag="ACIE68_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFX5906401.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22406..23266)
                     /gene="tesB"
                     /locus_tag="ACIE68_000020"
     CDS             complement(22406..23266)
                     /gene="tesB"
                     /locus_tag="ACIE68_000020"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFX5906402.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23484..24056
                     /gene="ybaY"
                     /locus_tag="ACIE68_000021"
     CDS             23484..24056
                     /gene="ybaY"
                     /locus_tag="ACIE68_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFX5906403.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(24087..24398)
                     /locus_tag="ACIE68_000022"
     CDS             complement(24087..24398)
                     /locus_tag="ACIE68_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFX5906404.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            24561..24657
                     /gene="ffs"
                     /locus_tag="ACIE68_000023"
     ncRNA           24561..24657
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACIE68_000023"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24777..25130
                     /gene="ybaA"
                     /locus_tag="ACIE68_000024"
     CDS             24777..25130
                     /gene="ybaA"
                     /locus_tag="ACIE68_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFX5906405.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(25172..26722)
                     /gene="pdeB"
                     /locus_tag="ACIE68_000025"
     CDS             complement(25172..26722)
                     /gene="pdeB"
                     /locus_tag="ACIE68_000025"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFX5906406.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(26886..27356)
                     /gene="ylaC"
                     /locus_tag="ACIE68_000026"
     CDS             complement(26886..27356)
                     /gene="ylaC"
                     /locus_tag="ACIE68_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFX5906407.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(27472..28023)
                     /gene="maa"
                     /locus_tag="ACIE68_000027"
     CDS             complement(27472..28023)
                     /gene="maa"
                     /locus_tag="ACIE68_000027"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFX5906408.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPNNVVVGGNPARIIKKL"
     gene            complement(28195..28413)
                     /gene="hha"
                     /locus_tag="ACIE68_000028"
     CDS             complement(28195..28413)
                     /gene="hha"
                     /locus_tag="ACIE68_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFX5906409.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(28439..28813)
                     /gene="tomB"
                     /locus_tag="ACIE68_000029"
     CDS             complement(28439..28813)
                     /gene="tomB"
                     /locus_tag="ACIE68_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFX5906410.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(29359..32508)
                     /gene="acrB"
                     /locus_tag="ACIE68_000030"
     CDS             complement(29359..32508)
                     /gene="acrB"
                     /locus_tag="ACIE68_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFX5906411.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(32531..33724)
                     /gene="acrA"
                     /locus_tag="ACIE68_000031"
     CDS             complement(32531..33724)
                     /gene="acrA"
                     /locus_tag="ACIE68_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFX5906412.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            33866..34513
                     /gene="acrR"
                     /locus_tag="ACIE68_000032"
     CDS             33866..34513
                     /gene="acrR"
                     /locus_tag="ACIE68_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFX5906413.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            34641..38003
                     /gene="mscK"
                     /locus_tag="ACIE68_000033"
     CDS             34641..38003
                     /gene="mscK"
                     /locus_tag="ACIE68_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFX5906414.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            38064..38162
                     /locus_tag="ACIE68_000034"
                     /pseudo
     CDS             38064..38162
                     /locus_tag="ACIE68_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(38215..38376)
                     /gene="rsmS"
                     /locus_tag="ACIE68_000035"
     CDS             complement(38215..38376)
                     /gene="rsmS"
                     /locus_tag="ACIE68_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFX5906415.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(38390..38917)
                     /gene="priC"
                     /locus_tag="ACIE68_000036"
     CDS             complement(38390..38917)
                     /gene="priC"
                     /locus_tag="ACIE68_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFX5906416.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            38987..39364
                     /gene="ybaN"
                     /locus_tag="ACIE68_000037"
     CDS             38987..39364
                     /gene="ybaN"
                     /locus_tag="ACIE68_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFX5906417.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            39517..40068
                     /gene="apt"
                     /locus_tag="ACIE68_000038"
     CDS             39517..40068
                     /gene="apt"
                     /locus_tag="ACIE68_000038"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFX5906418.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            40197..42128
                     /gene="dnaX"
                     /locus_tag="ACIE68_000039"
     CDS             40197..42128
                     /gene="dnaX"
                     /locus_tag="ACIE68_000039"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFX5906419.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    41461..41525
                     /gene="dnaX"
                     /locus_tag="ACIE68_000039"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            42181..42510
                     /gene="ybaB"
                     /locus_tag="ACIE68_000040"
     CDS             42181..42510
                     /gene="ybaB"
                     /locus_tag="ACIE68_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFX5906420.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            42510..43115
                     /gene="recR"
                     /locus_tag="ACIE68_000041"
     CDS             42510..43115
                     /gene="recR"
                     /locus_tag="ACIE68_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFX5906421.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            43225..45099
                     /gene="htpG"
                     /locus_tag="ACIE68_000042"
     CDS             43225..45099
                     /gene="htpG"
                     /locus_tag="ACIE68_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFX5906422.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            45280..45924
                     /gene="adk"
                     /locus_tag="ACIE68_000043"
     CDS             45280..45924
                     /gene="adk"
                     /locus_tag="ACIE68_000043"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFX5906423.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            46160..47122
                     /gene="hemH"
                     /locus_tag="ACIE68_000044"
     CDS             46160..47122
                     /gene="hemH"
                     /locus_tag="ACIE68_000044"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFX5906424.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(47119..48078)
                     /gene="aes"
                     /locus_tag="ACIE68_000045"
     CDS             complement(47119..48078)
                     /gene="aes"
                     /locus_tag="ACIE68_000045"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFX5906425.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQSDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            48230..49534
                     /gene="gsk"
                     /locus_tag="ACIE68_000046"
     CDS             48230..49534
                     /gene="gsk"
                     /locus_tag="ACIE68_000046"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFX5906426.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(49667..51343)
                     /gene="ybaL"
                     /locus_tag="ACIE68_000047"
     CDS             complement(49667..51343)
                     /gene="ybaL"
                     /locus_tag="ACIE68_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFX5906427.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(51581..52801)
                     /gene="fsr"
                     /locus_tag="ACIE68_000048"
     CDS             complement(51581..52801)
                     /gene="fsr"
                     /locus_tag="ACIE68_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFX5906428.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            53019..54671
                     /gene="ushA"
                     /locus_tag="ACIE68_000049"
     CDS             53019..54671
                     /gene="ushA"
                     /locus_tag="ACIE68_000049"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFX5906429.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(54708..55187)
                     /gene="ybaK"
                     /locus_tag="ACIE68_000050"
     CDS             complement(54708..55187)
                     /gene="ybaK"
                     /locus_tag="ACIE68_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFX5906430.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(55391..56185)
                     /gene="ybaP"
                     /locus_tag="ACIE68_000051"
     CDS             complement(55391..56185)
                     /gene="ybaP"
                     /locus_tag="ACIE68_000051"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFX5906431.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            56323..56664
                     /gene="ybaQ"
                     /locus_tag="ACIE68_000052"
     CDS             56323..56664
                     /gene="ybaQ"
                     /locus_tag="ACIE68_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFX5906432.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(56879..59383)
                     /gene="copA"
                     /locus_tag="ACIE68_000053"
     CDS             complement(56879..59383)
                     /gene="copA"
                     /locus_tag="ACIE68_000053"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFX5906433.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            59645..60577
                     /gene="glsA"
                     /locus_tag="ACIE68_000054"
     CDS             59645..60577
                     /gene="glsA"
                     /locus_tag="ACIE68_000054"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFX5906434.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGKVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            60580..61872
                     /gene="ybaT"
                     /locus_tag="ACIE68_000055"
     CDS             60580..61872
                     /gene="ybaT"
                     /locus_tag="ACIE68_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFX5906435.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            61903..62000
                     /locus_tag="ACIE68_000056"
                     /pseudo
     CDS             61903..62000
                     /locus_tag="ACIE68_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            61997..62404
                     /gene="cueR"
                     /locus_tag="ACIE68_000057"
     CDS             61997..62404
                     /gene="cueR"
                     /locus_tag="ACIE68_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFX5906436.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(62405..62863)
                     /gene="ybbJ"
                     /locus_tag="ACIE68_000058"
     CDS             complement(62405..62863)
                     /gene="ybbJ"
                     /locus_tag="ACIE68_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFX5906437.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(62860..63777)
                     /gene="qmcA"
                     /locus_tag="ACIE68_000059"
     CDS             complement(62860..63777)
                     /gene="qmcA"
                     /locus_tag="ACIE68_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFX5906438.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            63923..64600
                     /gene="fetA"
                     /locus_tag="ACIE68_000060"
     CDS             63923..64600
                     /gene="fetA"
                     /locus_tag="ACIE68_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFX5906439.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKDIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            64587..65366
                     /gene="fetB"
                     /locus_tag="ACIE68_000061"
     CDS             64587..65366
                     /gene="fetB"
                     /locus_tag="ACIE68_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFX5906440.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFVAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(65429..66283)
                     /gene="cnoX"
                     /locus_tag="ACIE68_000062"
     CDS             complement(65429..66283)
                     /gene="cnoX"
                     /locus_tag="ACIE68_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFX5906441.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(66344..67153)
                     /gene="ybbO"
                     /locus_tag="ACIE68_000063"
     CDS             complement(66344..67153)
                     /gene="ybbO"
                     /locus_tag="ACIE68_000063"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFX5906442.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(67143..67766)
                     /gene="tesA"
                     /locus_tag="ACIE68_000064"
     CDS             complement(67143..67766)
                     /gene="tesA"
                     /locus_tag="ACIE68_000064"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFX5906443.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            67737..68423
                     /gene="ybbA"
                     /locus_tag="ACIE68_000065"
     CDS             67737..68423
                     /gene="ybbA"
                     /locus_tag="ACIE68_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFX5906444.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            68420..70834
                     /gene="ybbP"
                     /locus_tag="ACIE68_000066"
     CDS             68420..70834
                     /gene="ybbP"
                     /locus_tag="ACIE68_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFX5906445.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFFSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(71052..71207)
                     /gene="dinQ"
                     /locus_tag="ACIE68_000067"
     CDS             complement(71052..71207)
                     /gene="dinQ"
                     /locus_tag="ACIE68_000067"
                     /inference="COORDINATES: protein motif:HMM:NF041472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFX5906446.1"
                     /translation="MKKIIVTGNVRVSCDCLSPLREQRMKTFLDKVLIVLKALIALLE
                     LIRQFIE"
     gene            complement(71546..72640)
                     /gene="mnmH"
                     /locus_tag="ACIE68_000068"
     CDS             complement(71546..72640)
                     /gene="mnmH"
                     /locus_tag="ACIE68_000068"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFX5906447.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEHGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEVGINYPLVEGGYKVLRQTAIQATIELSQKPIVLIGGCTGCGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            complement(72709..73635)
                     /gene="allS"
                     /locus_tag="ACIE68_000069"
     CDS             complement(72709..73635)
                     /gene="allS"
                     /locus_tag="ACIE68_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFX5906448.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            73865..74347
                     /gene="allA"
                     /locus_tag="ACIE68_000070"
     CDS             73865..74347
                     /gene="allA"
                     /locus_tag="ACIE68_000070"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFX5906449.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            74425..75240
                     /gene="allR"
                     /locus_tag="ACIE68_000071"
     CDS             74425..75240
                     /gene="allR"
                     /locus_tag="ACIE68_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFX5906450.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIVILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            75330..77111
                     /gene="gcl"
                     /locus_tag="ACIE68_000072"
     CDS             75330..77111
                     /gene="gcl"
                     /locus_tag="ACIE68_000072"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFX5906451.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            77124..77900
                     /gene="hyi"
                     /locus_tag="ACIE68_000073"
     CDS             77124..77900
                     /gene="hyi"
                     /locus_tag="ACIE68_000073"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFX5906452.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            78000..78878
                     /gene="glxR"
                     /locus_tag="ACIE68_000074"
     CDS             78000..78878
                     /gene="glxR"
                     /locus_tag="ACIE68_000074"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFX5906453.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            79047..80501
                     /gene="ybbW"
                     /locus_tag="ACIE68_000075"
     CDS             79047..80501
                     /gene="ybbW"
                     /locus_tag="ACIE68_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFX5906454.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGKINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            80561..81922
                     /gene="allB"
                     /locus_tag="ACIE68_000076"
     CDS             80561..81922
                     /gene="allB"
                     /locus_tag="ACIE68_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFX5906455.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            81979..83280
                     /gene="ybbY"
                     /locus_tag="ACIE68_000077"
     CDS             81979..83280
                     /gene="ybbY"
                     /locus_tag="ACIE68_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFX5906456.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            83302..84447
                     /gene="glxK"
                     /locus_tag="ACIE68_000078"
     CDS             83302..84447
                     /gene="glxK"
                     /locus_tag="ACIE68_000078"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFX5906457.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANSHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(84675..85460)
                     /gene="allE"
                     /locus_tag="ACIE68_000079"
     CDS             complement(84675..85460)
                     /gene="allE"
                     /locus_tag="ACIE68_000079"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFX5906458.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(85471..86706)
                     /gene="allC"
                     /locus_tag="ACIE68_000080"
     CDS             complement(85471..86706)
                     /gene="allC"
                     /locus_tag="ACIE68_000080"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFX5906459.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHNGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(86728..87777)
                     /gene="allD"
                     /locus_tag="ACIE68_000081"
     CDS             complement(86728..87777)
                     /gene="allD"
                     /locus_tag="ACIE68_000081"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFX5906460.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGNEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
BASE COUNT        21301 a        22087 c        23422 g        21112 t
ORIGIN      
        1 gaaaataaag actcattagc tgaatcgtgt aagaaaaata gaatttataa atggcgtcaa
       61 taatttccaa acgctaagcc gcacaaaaga acaaatatta ttaagcgata ctgatgttta
      121 tttagccgta ataattacgg cgagtgatta ctacagctaa ataatattta cagattacgt
      181 cagatgcgtt tttcgtagcg ccagataatc cagcaaacca ccaagcacga cgccagaaag
      241 ggcgactaaa accccgactt ccagcagagc aggcaggaaa gagaagtgcg tcaaatccag
      301 cgcgtccatc gtcaacagca gtaaccacac ggccaacagg ctgacgcccg ccgccagtgt
      361 ccacatggca aaggcataac ctgccggata ttcggtacgg gagataaagt tgtcatttac
      421 tcgtgtatat tcaagcgtct ggcggcaaac cagcagcaaa ataagccccg gtacggcagc
      481 ggcgacggag aatagataga atgtcgacca gccgtgtgct tcaacaaacc aacccgccac
      541 ggggccgaca taaactcgtc ctacagcgga aagcgctgag agcagggcaa attgagtagc
      601 ggaaaatgac ttattacaca gcgtcattaa cagcgcgaca aaggctgatg tgcccatccc
      661 gccacagagg ttttcgaaaa agacggctgc gcccatgctg tagagatgct tatcagtaat
      721 cgacagcagc cagtaaccag cgttagacgc accttgtaaa atgccgaaaa tcagcagcgc
      781 ccggaacagc gacaggcgct gcatcaaaat cccaccgtac aatgcgccca caatggtcgc
      841 cagtaagcca agcgttttgt ttaccacgcc tacttcaccc gcatcaaacc cgacgccgcg
      901 aatcaaaaac gtggttgtca ggctcatggc gaatgcgtcg cccagcttat acagcacgat
      961 aagaagcaaa ataagccagg cattattgcg accaaagaaa tctcgcagag gtgcaacaac
     1021 cgcttgttcc agcgttttgg gcacaggaat ggtgtcggtt ggttctggtg caagcaacgt
     1081 cgcgataata caggggatca acagcgccgc catcaaccag tacatgccct gccagcccag
     1141 ccatttatct gccagccaca gggctaggcc gccggaaacc agcatcccta aacggtaacc
     1201 cagcacgctg attgccgcgc ccgcaccacg ctcttctgcc gggagcacat cggttttcca
     1261 cgcatcgaag acaatatcct gagaggccga gcaaaaagcg atcaccaccg ccagcgccgc
     1321 catccagcgg agttgggtgc ctggttcgag aaaacccatc gccgcaatgg cgactaataa
     1381 caggatttgc gtggcgagca gccaaccgcg ccgccgccca aaaaatgggg gcgtgtagcg
     1441 gtccatcagc ggtgaccaga ggaatttaaa aacgtaagcc tggcctacca gagagaagaa
     1501 accaatggtt ttgagatcaa tattctcgac cgtcatccag gcctgtaagg taccggatgt
     1561 cagggcgagc ggtagcccgg aagcaaaacc caggatcagc aatatggctg aacgcggctg
     1621 ttgaaaaata cgtaaatatt gactggacat gggcgtctac aggcccggct tgcaccgggc
     1681 cagagggtag aaattaacgc gcgttctgct tgatgaattc gtggatgctg gtgtcctgag
     1741 acatatcagc gatggtatcg gtcagcacac tgttaaccgc atcggcgata tttttgttgg
     1801 aggcctggaa cgcaccttca acgttgtagc tggcacgata gtttttggtc attttgttgc
     1861 cattctgcgc ggtagcgatg atggcgatat ccgctttggt cgcgatgttg tagcgcacgt
     1921 tgccctggga cacgtcagca tacagttggc taacgatgat ttgcagatta accgggccat
     1981 tcggaccaac catgtaacca cgcgcggtca tctgtttttc cagcacttct tgcagcagga
     2041 aacgcagatc gcgggaggcg gtcagggtaa cgatttgatt atcgcgggtg acttttgcca
     2101 gcgcctgatc ggtacgctga tcggcaccat taatgcttac ggtgacgccc atcaggcttg
     2161 gatcctgctg tggcagtgta atcgtcgggg aaacttcaat agttgttggc ggttttgcgc
     2221 atcctgcaag cataaacaga gcaactaacg ggaagaggat ttttttgaac atgttcgggc
     2281 tctcagagac tcttaagcgt gtttggtaaa aattcccgcc atcataacat tgccaacggc
     2341 gaggggaagt gggtaaggca tgtaaattca tcatgttgac gaaataatcg cccctggtaa
     2401 aagaaacact gatgcgaggc ctgtgtttca atctttaaat cagtaaactt catacgcttg
     2461 acggaaaaac caggacgaaa cctaaatatt tgttgttaag ctgcaatgga aacggtaaaa
     2521 gcggctagta tttaaaggga tggatgacat ctcagcgttg tcggaggaga tatttcatga
     2581 tgatacgtga gcggatagaa gaaaaattaa gggcggcgtt ccaacccgta ttcctcgaag
     2641 tagtggatga aagctatcgt cacaatgtcc cagccggctc tgaaagccat tttaaagttg
     2701 tgctggtcag cgatcgtttt acgggtgaac gttttctgaa tcgtcatcga atgatttaca
     2761 gtactttagc ggaggaactc tctactaccg ttcatgcgct ggctctgcat acttacacta
     2821 ttaaggagtg ggaagggttg caggacaccg tctttgcctc tcctccctgt cgtggagcag
     2881 gaagcatcgc gtaaaaacgc atttgcaact gtcggcgctt ttccagtatg ttgctaaaga
     2941 ttttatgaaa aacggcctgc gggccgtttt gttttgtctg gattttgcgc tttttgccca
     3001 gcattcagac gaaaattgcc cgggaattgt gaaaaaatac gcgacagcgc gcaataaccg
     3061 ttctcgactc ataaaagtga tgccgctata atgccgcgtc ctatttgaat gctttcggga
     3121 tgattctggt aacagggaat gtgattgatt ataagaacat cccggttccg cgaagccaac
     3181 aacctgtgct tgcggggtaa gagttgaccg agcactgtga ttttttgagg taacaagatg
     3241 caagtttcag ttgaaaccac tcaaggcctt ggccgccgtg taacgattac tatcgctgct
     3301 gacagcatcg agaccgctgt taaaagcgag ctggtcaacg ttgcgaaaaa agtacgtatt
     3361 gacggcttcc gcaaaggcaa agtgccaatg aatatcgttg ctcagcgtta tggcgcgtct
     3421 gtacgccagg acgttctggg tgacctgatg agccgtaact tcattgacgc catcattaaa
     3481 gaaaaaatca atccggctgg cgcaccgact tatgttccgg gcgaatacaa gctgggtgaa
     3541 gactttactt actctgtaga gtttgaagtt tatccggaag ttgaactgca gggtctggaa
     3601 gcgatcgaag ttgaaaaacc gatcgttgaa gtgaccgacg ctgacgttga cggcatgctg
     3661 gatactctgc gtaaacagca ggcgacctgg aaagaaaaag acggcgctgt tgaagcagaa
     3721 gaccgcgtaa ccatcgactt caccggttct gtagacggcg aagagttcga aggcggtaaa
     3781 gcgtctgatt tcgtactggc gatgggccag ggtcgtatga tcccgggctt tgaagacggt
     3841 atcaaaggcc acaaagctgg cgaagagttc accatcgacg tgaccttccc ggaagaatac
     3901 cacgcagaaa acctgaaagg taaagcagcg aaattcgcta tcaacctgaa gaaagttgaa
     3961 gagcgtgaac tgccggaact gactgcagaa ttcatcaaac gtttcggcgt tgaagatggt
     4021 tccgtagaag gtctgcgcgc tgaagtgcgt aaaaacatgg agcgcgagct gaagagcgcc
     4081 atccgtaacc gcgttaagtc tcaggcgatc gaaggtctgg taaaagctaa cgacatcgac
     4141 gtaccggctg cgctgatcga cagcgaaatc gacgttctgc gtcgccaggc tgcacagcgt
     4201 ttcggtggca acgaaaaaca agctctggaa ctgccgcgcg aactgttcga agaacaggct
     4261 aaacgccgcg tagttgttgg cctgctgctg ggcgaagtta tccgcaccaa cgagctgaaa
     4321 gctgacgaag agcgcgtgaa aggcctgatc gaagagatgg cttctgcgta cgaagatccg
     4381 aaagaagtta tcgagttcta cagcaaaaac aaagaactga tggacaacat gcgcaatgtt
     4441 gctctggaag aacaggctgt tgaagctgta ctggcgaaag cgaaagtgac tgaaaaagaa
     4501 accactttca acgagctgat gaaccagcag gcgtaattta cgcagcataa cgcgctaaat
     4561 tcgcacaaag gcccgtcacc gccaggtggt gggctttttt ttgtcatgaa ttttgcatgg
     4621 aaccgtgcga aaagcctctt tcggtgttag cgtaacaaca aaagattgtt atgcttgaaa
     4681 tatggtgatg ccgtacccat aacacaggga ctagctgata atccgtccat aaggttacaa
     4741 tcggtacagc aggttttttc aattttatcc aggagacgga aatgtcatac agcggcgaac
     4801 gagataactt tgcaccccat atggcgctgg tgccgatggt cattgaacag acctcacgcg
     4861 gtgagcgctc ttttgatatc tattctcgtc tacttaagga acgcgtcatt tttctgactg
     4921 gccaggttga agaccacatg gctaacctga ttgtggcgca gatgctgttc ctggaagcgg
     4981 aaaacccaga aaaagatatc tatctgtaca ttaactcccc aggcggggtg atcactgccg
     5041 ggatgtctat ctatgacacc atgcagttta tcaagcctga tgtcagcacc atctgtatgg
     5101 gccaggcggc ctcgatgggc gctttcttgc tgaccgcagg ggcaaaaggt aaacgttttt
     5161 gcctgccgaa ttcgcgcgtg atgattcacc aaccgttggg cggctaccag ggccaggcga
     5221 ccgatatcga aattcatgcc cgtgaaattc tgaaagttaa agggcgcatg aatgaactta
     5281 tggcgcttca tacgggtcaa tcattagaac agattgaacg tgataccgag cgcgatcgct
     5341 tcctttccgc ccctgaagcg gtggaatacg gtctggtcga ttcgattctg acccatcgta
     5401 attgatgcca gaggcgcaac tgtgccgcta tacttatcca gggcggcaca acgctgtaag
     5461 cggcttgcgc ctgagaatgg catttgcgtc gtcgtgtgcg gcacaaagaa caaagaagag
     5521 gttttgaccc atgacagata aacgcaaaga tggctcaggc aaattgctgt attgctcttt
     5581 ttgcggcaaa agccagcatg aagtgcgcaa gctgattgcc ggtccatccg tgtatatctg
     5641 cgacgaatgt gttgatttat gtaacgacat cattcgcgaa gagattaaag aagttgcacc
     5701 gcatcgtgaa cgcagtgcgc taccgacgcc gcatgaaatt cgcaaccacc tggacgatta
     5761 cgttatcggc caggaacagg cgaaaaaagt gctggcggtc gcggtataca accattacaa
     5821 acgtctgcgc aacggcgata ccagcaatgg cgtcgagttg ggcaaaagta acattctgct
     5881 gatcggtccg accggttccg gtaaaacgct gctggctgaa acgctggcgc gcctgctgga
     5941 tgttccgttc accatggccg acgcgactac actgaccgaa gccggttatg tgggtgaaga
     6001 cgttgaaaac atcattcaga agctgttgca gaaatgcgac tacgatgtcc agaaagcaca
     6061 gcgtggtatt gtctacatcg atgaaatcga caagatttct cgtaagtcag acaacccgtc
     6121 cattacccga gacgtttccg gtgaaggcgt acagcaggca ctgttgaaac tgatcgaagg
     6181 tacggtagct gctgttccac cgcaaggtgg gcgtaaacat ccgcagcagg aattcttgca
     6241 ggttgatacc tctaagatcc tgtttatttg tggcggtgcg tttgccggtc tggataaagt
     6301 gatttcccat cgtgtagaaa ccggctccgg cattggtttt ggcgcgacgg taaaagcgaa
     6361 gtccgacaaa gcaagcgaag gcgagctgct ggcgcaggtt gaaccggaag atctgatcaa
     6421 gtttggtctg atccctgagt tcattggtcg tctgccggtt gtcgcaacgt tgaatgaact
     6481 gagcgaagaa gctctgattc agatcctcaa agagccgaaa aacgccctga ccaagcagta
     6541 tcaggcgctg tttaatctgg aaggcgtgga tctggaattc cgtgacgagg cgctggatgc
     6601 tatcgctaag aaagcgatgg cgcgtaaaac cggtgcccgt ggcctgcgtt ccatcgtaga
     6661 agccgcactg ctcgatacca tgtacgatct gccgtccatg gaagacgtcg aaaaagtggt
     6721 tatcgacgag tcggtaattg atggtcaaag caaaccgttg ctgatttatg gcaagccgga
     6781 agcgcaacag gcatctggtg aataattaac cattcccata caattagtta accaaaaagg
     6841 ggggatttta tctccccttt aatttttcct ctattctcgg cgttgaatgt gggggaaaca
     6901 tccccatata ctgacgtaca tgttaataga tggcgtgaag cacagtcgtg tcatctgatt
     6961 acctggcgga aattaaacta agagagagct ctatgaatcc tgagcgttct gaacgcattg
     7021 aaatccccgt attgccgctg cgcgatgtgg tggtttatcc gcacatggtc atccccttat
     7081 ttgtcgggcg ggaaaaatct atccgttgtc tggaagcggc gatggaccat gataaaaaaa
     7141 ttatgctggt cgcgcagaaa gaagcttcaa cggatgagcc gggtgtaaac gatcttttca
     7201 ccgtcgggac cgtggcctct atattgcaga tgctgaagct gcctgacggc accgtcaaag
     7261 tgctggtcga ggggttacag cgcgcgcgta tttctgcgct ctctgacaat ggcgaacact
     7321 tttctgcgaa ggcggagtat ctggagtcgc cgaccattga tgagcgagaa caggaagtgc
     7381 tggtgcgtac tgcaatcagc cagttcgaag gctacatcaa gctgaacaaa aaaatcccac
     7441 cagaagtgct gacgtcgttg aatagcatcg acgatccggc gcgtctggcg gataccattg
     7501 ctgcacatat gccgctgaaa ctggctgaca aacagtccgt tctggagatg tccgacgtta
     7561 acgaacgtct ggaatatctg atggcaatga tggaatcgga aatcgatctg ctgcaggttg
     7621 agaaacgcat tcgcaaccgc gttaaaaagc agatggagaa atcccagcgt gagtactatc
     7681 tgaacgagca aatgaaagct attcagaaag aactcggtga aatggacgac gcgccggacg
     7741 aaaacgaagc cctgaagcgc aaaatcgacg cggcgaagat gccgaaagaa gcaaaagaga
     7801 aagcggaagc agagttgcag aagctgaaaa tgatgtctcc gatgtcggca gaagcgaccg
     7861 tagtgcgtgg ttatatcgac tggatggtac aggtaccgtg gaatgcgcgc agcaaggtca
     7921 aaaaagacct gcgtcaggcg caggaaatcc ttgataccga ccattatggt ctggagcgcg
     7981 tgaaagatcg catccttgag taccttgcgg ttcaaagccg tgtcaacaaa atcaagggac
     8041 cgatcctctg cctggtaggg ccgccggggg taggtaaaac ctctcttggt cagtccatcg
     8101 ccaaagccac cgggcgtaaa tatgtccgta tggcgctggg cggcgtgcgt gatgaagcgg
     8161 aaatccgtgg tcaccgccgt acttacatcg gttctatgcc gggtaaactg atccagaaaa
     8221 tggcgaaagt gggcgtgaaa aacccgctgt tcctgctcga tgagatcgac aaaatgtctt
     8281 ctgacatgcg tggcgatccg gcctccgcac tgcttgaagt gctggatcca gagcagaacg
     8341 tagcgttcag cgaccactac ctggaagtgg attacgatct cagcgacgtg atgtttgtcg
     8401 cgacgtcgaa ctccatgaac attccggcac cgctgctgga tcgtatggaa gtgattcgcc
     8461 tctccggtta taccgaagat gaaaaactga acatcgccaa acgtcacctg ctgccgaagc
     8521 agattgaacg taatgcactg aaaaaaggtg agctgaccgt cgacgatagc gccattatcg
     8581 gcattattcg ttactacacc cgtgaggcgg gcgtgcgtgg tctggagcgt gaaatctcca
     8641 aactgtgccg caaagcggtt aagcagttac tgctcgataa gtcattaaaa catatcgaaa
     8701 ttaacggcga taacctgcat gactacctcg gtgttcagcg tttcgactat ggtcgcgctg
     8761 ataacgaaaa ccgtgtcggt caggtaaccg gtctggcgtg gacggaagtg ggcggtgact
     8821 tgctgaccat tgaaaccgca tgtgttccgg gtaaaggcaa actgacctat accggttcgc
     8881 tcggcgaagt gatgcaggag tctattcagg cggcgttaac ggtggttcgt gcgcgtgcgg
     8941 aaaaactggg gatcaaccct gatttttacg aaaaacgtga catccacgtc cacgtaccgg
     9001 aaggtgcgac gccgaaagat ggtccgagtg ccggtattgc tatgtgcacc gcgctggttt
     9061 cttgcctgac cggtaacccg gttcgtgccg atgtggcaat gaccggtgag atcactctgc
     9121 gtggtcaggt actgccgatc ggtggtttga aagaaaaact cctggcagcg catcgcggcg
     9181 ggattaaaac agtgctaatt ccgttcgaaa ataaacgcga tctggaagag attcctgaca
     9241 acgtaattgc cgatctggac attcatcctg tgaagcgcat tgaggaagtt ctgactctgg
     9301 cgctgcaaaa tgaaccgtct ggcatgcagg ttgtgactgc aaaatagtga cctcgcgcaa
     9361 aatgcactaa taaaaacagg gctggcaggc taattcgggc ttgccagcct ttttttgtct
     9421 cgctaagtta gatggcggat cgggcttgcc cttattaagg ggtgttgtaa ggggatggct
     9481 ggcctgatat aactgctgcg cgttcgtacc ttgaaggatt caagtgcgat ataaattata
     9541 aagaggaaga gaagagtgaa taaatctcaa ttgatcgaca agattgctgc aggggctgat
     9601 atctctaaag ctgcggctgg ccgtgcgtta gatgctatta ttgcttccgt aactgaatct
     9661 ctgaaagaag gggatgatgt agcactggta ggttttggta cttttgccgt taaagagcgt
     9721 gctgcccgta ctggccgcaa cccgcagacc ggtaaagaga tcaccatcgc tgctgctaaa
     9781 gtaccgagct tccgtgcagg taaagcactg aaagacgcgg taaactaagc gttgtccccg
     9841 gtggggatgt gacgaagttc aagggcgcat ctactgatgt gcctttttta tttgtattcg
     9901 gtgactttct gcgtcttgtg ggctgacaat tgcccccgtt tcttgtcaca ataggccttt
     9961 gcgcgcatcg atacgttgcg tgaggtacac agtcatctac agcggagtgt tgttacacca
    10021 tgatggacag cttacgcacg gctgcaaaca gtctcgtgct caagattatt ttcggtatca
    10081 ttatcgtgtc gttcatattg accggcgtga gtggttacct gattggcgga ggcaataact
    10141 acgccgcaaa agtgaatgac caggaaatca gccgtgggca gttcgagaac gcctttaaca
    10201 gcgagcgtaa tcgcatgcag caacagctgg gcgatcaata ctccgagctg gcagcgaacg
    10261 aaggctatat gaaaaccctg cgtcaacagg tgctgaatcg tctgatcgac gaggcgctgt
    10321 tggatcagta cgctcgtgag ctgaaactgg gtatcagtga tgagcaggtt aaacaggcga
    10381 ttttcgcgac cccagccttc caggttgacg gcaaatttga taacagccgc tataacggta
    10441 tcctcaacca gatggggatg accgccgatc agtacgccca ggcgctgcgt aaccagctca
    10501 ctacccaaca gctgattaac ggtgttgccg gtaccgattt tatgctgaaa ggtgaaaccg
    10561 acgagctggc ggcactggtc gcgcaacaac gcgtggtgcg tgaggcgact atcgatgtta
    10621 acgcgctggc ggcgaagcag cctgtgaccg aacaggagat tgccagctac tacgaacaaa
    10681 acaaaaacaa tttcatgacg ccggaacaat tccgcgtgag ttacatcaag ctggatgccg
    10741 caacgatgca gcaaccggtt agcgatgcgg atatccagag ctactacgat cagcatcagg
    10801 atcaattcac ccagccgcag cgtacccgct acagcatcat ccagaccaaa actgaagatg
    10861 aagcgaaagc ggtacttgat gagctgaata aaggcggtga ttttgctgca ttagccaaag
    10921 aaaaatctgc cgatattatc tctgcccgta acggcggcga tatgggttgg ttagaagatg
    10981 ccactatccc cgacgaactg aaaaatgctg gtctgaaaga aaaagggcaa ctgtctggtg
    11041 tcatcaaatc ttcggtcggt ttcctgattg tacgtctgga cgacattcag ccagcgaaag
    11101 tgaaatcgtt agacgaagta cgtgacgaca ttgcggcgaa agtgaaacac gaaaaagccc
    11161 tcgatgcgta ctatgcgctg cagcagaaag tgagcgatgc ggcaagcaac gacaccgagt
    11221 ctctggccgg tgcagagcaa actgccggcg ttaaagccac tcagacgggt tggttcagca
    11281 aagataacct gccggaagag ttgaacttca agccggttgc cgacgctatc tttaacggcg
    11341 gtctggtagg tgaaaacggc gcgccgggca tcaactctga catcatcacc gtagacggcg
    11401 accgcgcatt cgtgctgcgc atcagcgagc acaaaccgga agcggtgaaa ccgttggcag
    11461 atgttcagga acaagttaag gcattggttc agcacaacaa agctgaacaa caggcgaaag
    11521 tggatgctga gaaactgctg gttgatttga aagccggcaa aggtgcggaa gctatgcagg
    11581 ctgccggtct gaaatttggc gagccgaaaa ccttaagccg ttccggtcgt gacccgatta
    11641 gccaggcggc gtttgcactg ccactgccag cgaaagacaa accgagctac ggtatggcga
    11701 ccgatatgca aggtaatgtg gttctgctgg cgctggatga agtgaaacaa ggttcaatgc
    11761 cggaagatca gaaaaaagcg atggtgcagg gtatcaccca gaacaacgca caaatcgtct
    11821 ttgaagctct gatgagtaac ctgcgtgaag aggcgaaaat caaaattggc gatgcgctgg
    11881 aacagcaata atcctgaagc cgcctcgcaa aaaaatgcgt tgcagctgta acaagaaaag
    11941 gtcgctttcg cggccttttc catttctgaa cattgccatt tgtttactgt tttcactgcc
    12001 gttaaggtga ttccactgtt aacaaacaag gagaaaacag tatgaaacac ggaattaaag
    12061 cactgctcat taccctgtct ctggcttgtg ccggaatgtc tcatagcgcg ctggcggcag
    12121 ctcctgcggc gaaaccgaca acggtagaaa ccaaagcgga agctcctgca gcacaaagta
    12181 aagcagcagt accggcgaaa gccagtgacg aagaaggcac ccgggtcagc attaataatg
    12241 ccagcgcgga agagctagcc cgcgcgatga atggcgttgg cctgaagaaa gcgcaggcga
    12301 ttgtcagtta tcgcgaagag tacggtccgt ttaaaacggt ggaggatcta aagcaggtgc
    12361 cggggatggg caattcgctg gtggaacgta atctggcggt attaaccctg taattaattt
    12421 gcatagtggc aattttttgc cagactgaag aggtcatacc agttatgacc tctgtactta
    12481 taacaacaac gtaaggttat tgcgctatgc aaacacaaat caaagttcgt ggatatcatc
    12541 tcgacgttta ccagcacgtc aacaacgccc gctaccttga atttctcgaa gaagcccgct
    12601 gggatgggtt ggaaaatagc gacagttttc agtggatgac ggcccataac atcgccttcg
    12661 tcgtggtcaa tatcaatatt aactatcgtc gcccagcggt attaagtgac ctgttaacta
    12721 ttaccagtca gttgcagcaa ttaaacggta aaagcggcat cttaagccag gtcattacac
    12781 tggagccgga agggcaggtg gtagcggatg cgcttattac gtttgtttgt attgatctta
    12841 aaacgcagaa agcattagct ctggaagggg aattgcgcga aaagctggag cagatggtta
    12901 agtaaacgtt ttgtggtgcc ggatgctcaa gccgcatccg gcgacacccg gaataattac
    12961 ctcaacccgg ttttctgctt catcgctgcc atcaccgtcg gtttatcggc cagataatga
    13021 ttcaaaccgt tggcgcgtaa attacatgcc gcacaatgac cgcaaccgtc gcctttaatg
    13081 ccgttatagc aggtcaacgt ttcgttacgg actaaatcca gtttgccgta ataatctgcc
    13141 agcgcccagg tttccgcttt atcaatccac atcagcggcg tttcaaaacg aatatctttc
    13201 gccatgccca aactgacggc atggtttagt gctttcacaa actcatcgcg gcaatccggg
    13261 tagccggaga aatccgtttc acagacgccg gtaattacgg cttctgcttt tacctgatac
    13321 gcatatattg ccgccagcgt caggaacaaa atattacgcc ctgggacaaa cgtattcggg
    13381 atgccatcgg cttcaggttc ataatcaggc accggaatgc tgtcacgtgt caggctgctg
    13441 accgccagct cgttgagcag cgttacatca agaaccttat gcgcgcgtgc tcctagtttc
    13501 agcgccagtt cgcgtgccac gtcgatttct gcgcgatgtc gctgaccgta atcgaacgtc
    13561 acgcaatgga cttcatcata ttgttgtaat gcctgcacca gacaggtggt ggaatcctgg
    13621 cctccactga acacaacgac agcacgtttc atagataatc ctgcttgaac aataaaagcg
    13681 ttatggtaac gcctgcgatt aacccggacc agctattcat tgcgacggcg cgggaagcca
    13741 ggcttcggta aattcaaacc agccgcgcgg tgtcagtcgc acaccgttca cgccgggagg
    13801 ggcactaatg cgatagtgat agttgaacag cggcgtcagc gtcgcgtctg ccattaactg
    13861 gctaaaaacc gctttcaggg cattaaatcg gttttcctca tcaggcatta cttgcaccgc
    13921 gtccagcgtc gattgcagat gtgcatatgc tggagcgtcg aaaacatgtg gccatagtgg
    13981 atcgcaacgt agccattgct ccagagtata ttccggtgct tcgccaatta atctgtcgcc
    14041 catcatgagg tctgcgtgtg ccagtagggt cgtgtcgtcc cagttttttg cgttatgaaa
    14101 aataattgtg agttcacagc cttccgctgc cagtgtcgcc tgtaggcgtt ctgccatggt
    14161 atgaagttct atcggtaagt gataaaccag cgtcaaggtt ttcggtagtt tgacttcatc
    14221 cggtacttgc cagtgcggaa tagtccagcc tggcagtaat gcatgactgg cggtgatcag
    14281 gttttctccg acttctaacg tttgtaataa accggattga tgaataatgg agatcacttt
    14341 tcgcgcctgc cagagggaga gtcggggact tttgcgcaac gttaaatagc aaaaacctaa
    14401 actgatgcca ctactgacct ggctgacccg ttgcagctcc tccggtttgc cgatggtgat
    14461 ttgcacggga tgccgacaac tggttcccaa atctttttcg aaaagcggcg gagttatcca
    14521 gtactcaacc gctttaagca gcggatgacg caaatggtaa taatcatggc tctccaggcg
    14581 caccagctcc gctgtgaatt gtgttaagcg aaaaggacca gtgccgatca gtgggaattg
    14641 cggatgcgcc agatggctgc aatagctcgc cagccggtgc gcaagccagt aatcggggcg
    14701 atgtaaaaag aaggtcagac actgcggatg ggtgacttca atacgcttca cgctaataaa
    14761 taattgatcc agtgctggca gttgtaacag catcaataat cgctggtgta agtgtgaggc
    14821 ttttactgca tcgccgttat gccagtgtag ggttgaacga agataaaagt cccagcgtaa
    14881 cccgtcagta gaggtttccc agtgatgcgc taaatcgcca atcgggcgct gagtattatt
    14941 atcgaagcgg gtcaggccgg aaaatatctg cccggcgaga tgctgctcgg cacggccggg
    15001 caaaaagcct ggttgtagcg gttcgagcgg gcgatagtag ggaatacgca acgtgggtgt
    15061 atcgttttgc cattgtccgc ccataaacgg ctgtaacaga gtgcgcagct cacctggggc
    15121 cagttgcgcc agctccagca catcttgctg ctttccggtt tccagtgcct gttccatcat
    15181 cgcattgcgt agcgattccg gcgtgaccag aaagcgtaat tgtccgcgct ttccgcgtcc
    15241 tgactgcgcc tgccactcca gccatcccgc ctcctgtgcc tgacgcaaca gcgtacgaac
    15301 atggcgttcg ctgcaaaaac agcgttcggc cagttcgctg acggtgacgg tttgcggctt
    15361 tccggctgaa ggttgccaca gacgttgata ctggttaaga cggttgagca atcgcatata
    15421 aacccggaac aatattattt aactattcac tattacttcc gtatatatca ggtgatactc
    15481 aatcaccatt aaccgtgtca cagagtggag aaagaaatgg ctcgtctggc agcatttgat
    15541 atggatggca ctttattgat gcctgaccat catttaggtg agaaaaccct ctctactttg
    15601 gcgcgactgc gtgaacgcga cattaccctc acttttgcca cggggcgtca tgcgctggag
    15661 atgcagcata ttctcggggc gctatcgctg gatgcatatt tgattaccgg caacggaacg
    15721 cgcgtgcatt ctctggaagg tgaactttta catcgtgatg atttacctgc ggatgtcgcg
    15781 gagctggtgc tgtatcagca atgggatacc cgagccagca tgcatatctt caatgacgac
    15841 ggttggttta ccgggaaaga gatccctgcg ttgttgcagg catttgtcta tagcggtttt
    15901 cgttatcaga taattgatgt caaaaaaatg ccactcggca gcgtcaccaa gatctgcttc
    15961 tgtggcgatc acgacgatct tacacgcttg cagatccagc tatacgaagc attaggcgag
    16021 cgtgcacatt tgtgtttttc cgccacggat tgcctcgaag tgctgccggt gggctgcaat
    16081 aaaggcgctg cattgacggt gctgacccaa catttaggtt tatcgttgcg cgattgcatg
    16141 gcctttggtg atgcgatgaa cgatcgcgaa atgttaggca gcgtcggtag cggctttatt
    16201 atgggcaatg cgatgccgca actgcgcgcg gagctcccgc atttaccggt gattggacat
    16261 tgccgaaatc aggctgtctc tcactatttg acgcactggc tggactatcc acatctacct
    16321 tattcccccg aataacgaga tcccttccag caccgggcaa ttgcccggtt ttttttgcgt
    16381 tgaatttgtc attttgtgcc gtggtgttta aaccgcacag aataaattgt cgcgatttca
    16441 cctttaaaat agaattaaaa gagaaaaaaa ttctccgtgg aagggctatg ttagataaaa
    16501 ttgaccgtaa gctgctggcc ttactgcagc aggattgcac cctctctttg caggcactgg
    16561 ctgaagccgt taatctgaca accacccctt gctggaagcg cctgaaacgg ctggaggacg
    16621 acggtatcct tatcggcaaa gtcgccctgc tggatccgga aaaaataggc ctcggcctga
    16681 ccgcctttgt gctgataaaa acgcaacatc acagcagcga atggtattgc cgctttgtca
    16741 cggtggttac cgaaatgcca gaagtgctgg ggttctggcg catggctggt gaatacgatt
    16801 atctgatgcg cgtccaggtt gccgacatga aacgctacga cgagttttat aagcgtctgg
    16861 taaacagcgt gccggggctg tcggacgtca cttccagctt cgcgatggaa cagattaaat
    16921 acaccacttc tttacccatc gaataaatat ccagaatcag gtcaggacac aacgcgtgcg
    16981 attatttgct caattaagct ggtatttccg tcgggaatgg cgtcgctatc tcggggctgt
    17041 cgccttgctt gtcattatcg cgatgctgca actggttccg ccaaaagtgg ttggtattgt
    17101 tgtcgatggc gtgacagaac aacactttac taccgggcag atcctgatgt ggatcgccac
    17161 catggtgctg attgccgttg tggtttatct cctgcgttac gtctggcggg tattgctgtt
    17221 tggtgcgtct tatcaactgg ctgttgaact gcgtgaagat tattaccgtc agctaagccg
    17281 gcagcatcct gagttttacc tgcgtcatcg cacgggtgac ctcatggctc gtgcgactaa
    17341 tgacgtcgat cgcgtcgtgt ttgccgccgg agaaggggtg ctgacgctgg tggattcact
    17401 ggtaatgggc tgcgctgtgt tgattatgat gtctacgcaa attagctggc agttgacctt
    17461 atttgccctg ttaccgatgc cagtgatggc gatcatgatt aagcgcaacg gcgatgcctt
    17521 gcatgaacgc tttaagctgg cacaggcggc gttttctagt cttaatgacc gcacccagga
    17581 aagcctcacc agtattcgca tgatcaaagc ctttggtctg gaagatcgcc agtcggcgtt
    17641 atttgccgcg gatgccgaag ataccggcaa aaaaaacatg cgggtggcgc gtattgatgc
    17701 tcgtttcgac ccgaccatct atatcgcgat tggtatggcg aacttgctgg cgattggcgg
    17761 cggtagttgg atggtggtgc agggcagttt aacgctgggc cagctcacca gttttatgat
    17821 gtatttaggt ctgatgattt ggccaatgct ggcgctggca tggatgttta acattgtgga
    17881 acgtggtagt gctgcgtaca gccgtattcg cgcgatgctg gcggaagcgc cggtggtgaa
    17941 cgatggtagc gaacccgtgc cggaagggcg tggcgaactg gatgtaaata ttcaccagtt
    18001 cacgtatccg cagactgacc atcctgcgct ggaaaacgtc aatttcgccc tgaaacccgg
    18061 tcagatgctg ggtatttgcg ggccgactgg ttccggcaaa agtaccctgt tgtcgctcat
    18121 tcagcgtcat ttcgacgtca gcgaggggga tattcgcttt catgatattc ctctgacgaa
    18181 gttacaactc gatagctggc gtagccgcct ggcggtggtc agccagacgc cattcctttt
    18241 ttctgacacc gtggcgaata acatcgcgct gggttgcccg aatgccaccc agcaagagat
    18301 tgaacacgta gcgcggttag ccagcgtaca tgacgatatt ttgcgtctgc cgcaaggtta
    18361 cgatacagaa gtgggcgagc gcggcgtgat gctttccggt gggcaaaaac agcgtatctc
    18421 cattgctcga gcgttattag tcaacgcgga aatcctcatc cttgatgatg cgctttcggc
    18481 ggtggacgga cgcacagagc accagatcct gcataacctg cgtcagtggg gacagggaag
    18541 aacggtaatc atcagtgccc atcgcctttc ggcactgacg gaagccagtg aaattattgt
    18601 gatgcagcac ggacatatcg cccagcgtgg caatcatgat gagctggcgc aacaaagcgg
    18661 ctggtatcgc gatatgtatc gctatcaaca actggaggcg gcgctcgacg acgttccgga
    18721 aattcgcgag gaggccgtcg atgcgtagtt ttagccaact gtggccgact ctcaagcgtc
    18781 tgttagcgta cggttcgccg tggcggaaac cgctggggat tgcggtcctg atgatgtggg
    18841 ttgcggcagc ggcagaagtc agtgggccgc tgcttatcag ctattttatc gacaatatgg
    18901 tagcgaaaaa taacctgccg ttgaaaatgg ttgcagggct ggcggcggcg tatgttgggc
    18961 tacaactgtt tgccgccggg ctacattacg cgcagtcgct gctgtttaat cgggcggcag
    19021 taggcgtagt gcaacagttg cgtaccgacg tgatggatgc tgcgttacgc cagccattaa
    19081 gcgagtttga tacccaaccc gtcgggcagg tgatttcccg cgtcactaat gacactgaag
    19141 tgatccgcga tctctacgtt accgtagtgg caactgtcct gcgtagcgcc gcactggtgg
    19201 gcgcgatgct ggtggcaatg ttcagcctcg actggcgaat ggcgctggtg gcgataatga
    19261 ttttcccggt agtgctggtg gtaatggtga tatatcaacg ttacagcacg ccgattgtcc
    19321 gtcgtgtgcg cgcctatctg gcggatatca acgacggctt taacgaaatc atcaatggca
    19381 tgagcgttat ccagcagttc cgtcagcagg cgcgatttgg cgaacgtatg ggtgaggcca
    19441 gtcgttcaca ctatatggcg aggatgcaaa ccctgcgcct cgacggtttt ctgctgcgtc
    19501 cgctgctgag tctgttttca tcgctcattc tttgtggctt gttgatgctg tttggcttct
    19561 ccgccagcgg caccattgaa gtgggcgtgt tatatgcgtt tatcagctat cttgggcgac
    19621 ttaacgaacc cttaatcgaa ctgaccacgc agcaggcgat gctgcaacag gctgttgttg
    19681 ctggtgagcg cgtgtttgaa ctgatggacg gaccgcgcca gcaatatggc aatgatgatc
    19741 gcccgttaca gagcggcacc atcgaagtcg ataacgtgtc atttgcttat cgcgatgaca
    19801 atctggtgct aaagaacatt aatctctctg tgccttcgcg taactttgtg gcgctggtcg
    19861 ggcataccgg cagtggcaaa agcaccctcg ccagtttatt gatgggctat tacccgctaa
    19921 cggaaggtga gattcgcctt gatggtcgtc cattaagttc gctaagtcac agcgcgctgc
    19981 gccagggcgt ggcaatggtg cagcaagatc cggtggtgct ggcggatacc ttcctcgcca
    20041 acgtgacgct gggacgtgat atctccgaag aacgcgtctg gcaggcgctg gaaaccgtgc
    20101 aactggcgga gctggcgcgc agcatgagtg atggtattta tacgccgctg ggcgagcagg
    20161 gaaataatct ctcagtcggg caaaagcaac tgctggcact ggcgcgcgtg ctggtggaga
    20221 cgccgcaaat cctgatcctt gatgaggcaa ccgccagcat tgactccggt actgaacagg
    20281 cgattcaaca tgctctggcg gcggtgcgtg aacataccac gctggtggtg attgctcacc
    20341 gcttatcaac tattgttgat gccgacacca ttctggtgct tcatcgtgga caagccgtgg
    20401 agcagggcac tcaccagcaa ctgctggcgg cccagggacg ctactggcag atgtatcaac
    20461 tgcaacttgc gggcgaagag ctggcagcca gcgtgcgtga agaggaatca ttgagcgcct
    20521 gaatagcgca atatttcatc gttggtgcaa aaatgtaacg cactgtgcac tgtcatagtg
    20581 cgttttcatt ttcaatcttc ttaacttcct gctctctttc tcatttttca tttctggcac
    20641 accgcttgca atatcttctt cgtgtagcag aaccattacc gaattctgac cggaggggat
    20701 ctatgaagct ggtgaccgtg ataatcaaac cattcaagct ggaagacgtt cgtgaagcgt
    20761 tatcttccat tggtattcag ggcctgaccg tcaccgaagt gaaaggtttc gggcgtcaga
    20821 aagggcatgc cgagctgtac cggggggcgg aatacagcgt caacttcctg ccaaaagtaa
    20881 aaattgatgt ggcgattgct gatgaccaac tcgatgaagt gatcgatatc gtcagtaagg
    20941 cggcttacac cggaaaaatt ggcgacggca aaatcttcgt cgctgaattg caacgcgtca
    21001 ttcgtattcg taccggcgaa gccgacgaag cggcgctgta atctctggca cacagcaaca
    21061 ggaacgaaaa atgaagatag cgacgataaa aactgggctt gcttcactgg cgatgctgcc
    21121 gggactggta atggctgcac ctgcggtggc cgataaagcc gacaatgcgt ttatgatgat
    21181 ttgtactgcg ctggtgctgt ttatgactat tccggggatt gccctgtttt acggtgggtt
    21241 gattcgcggc aaaaacgtgc tgtcgatgct gacgcaggtg acggtgacgt ttgcactggt
    21301 ctgtattctc tgggtggttt acggttactc gctggcgttt ggtgagggca acaacttctt
    21361 cggcaacatt aactggttga tgctaaaaaa catcgaactg acggcggtga tgggcagcat
    21421 ttatcagtat atccacgtgg cgtttcaggg ctcgtttgcc tgcattaccg tcggcttgat
    21481 cgttggggcg ctggcggaac gaatccgctt ctcagctgtg ttgattttcg tggtggtatg
    21541 gctgacgctc tcttacattc cgattgcgca tatggtgtgg ggcggtggtt tgctggcttc
    21601 tcacggtgcg ctggatttcg cgggtggcac ggtggtgcat attaacgccg caatcgccgg
    21661 tctggtgggc gcgtatctga taggcaaacg cgtgggcttc ggcaaagagg cgtttaaacc
    21721 gcacaacctg ccaatggtct tcactggtac tgccattctc tatattggtt ggtttggctt
    21781 taacgccggg tcagcgggca cggcgaatga aatcgcggca ctggcatttg tgaatactgt
    21841 ggtcgcaacg gcggcggcaa ttcttggctg gatcttcggt gaatgggcgc tgcgtggtaa
    21901 gccttctctg ctgggagcgt gttctggcgc gattgccggt ctggtcggcg tgacgccagc
    21961 gtgtggctac attggggttg gcggcgcgtt gattatcggc gtggtagctg gtctggcggg
    22021 cttgtggggc gttactatgc tcaaacgctt gctgcgggtg gatgatccct gcgatgtctt
    22081 cggtgtgcac ggcgtttgtg gcattgtcgg ctgtatcatg actgggattt ttgccgccag
    22141 ttcgctgggt ggcgtgggct tcgctgaagg tgtgacgatg ggccatcagt tgctggtaca
    22201 gctggaaagc atcgccatta cgatcgtctg gtccggtgtg gtggcattta ttggctacaa
    22261 actggcggat ctgacggttg gcctacgtgt gccggaagaa caagagcgag aagggctgga
    22321 cgtcaacagc cacggcgaga atgcctataa cgcgtaacaa gcactgcaaa aaatagccgg
    22381 acggttttca cctccggcta tttttttaat tgtgattacg cattacccct tcctgaacgg
    22441 tcgaggcaac cagcacgccg tcctgggtat aaaactcacc gcgcacaaag ccgcgtgcgc
    22501 tggacgccga ggtgctctcc acgctataca gcagccattc attcaaatta aacgggcgat
    22561 ggaaccacat ggaatggtca atggtggcaa tctgaatccc tggttcgaga aaaccgatgc
    22621 cgtgcggctg tagagctacc ggcaggaagt taagatcaga agcgtaaccg agcagatact
    22681 gatgaacgcg caggtcatcc ggcacgctac catttgcgcg gatccacacc tgacgatgtg
    22741 gttctgcgac gtgacctttc agtgggttat gaaactccac cggacggact tccagcggac
    22801 gatcgcagat gaatttatct ttaagcactg gcggcagcag gtgcgccagc gattgggcga
    22861 tttgcgtttc cgaaggcagg ccatcaggcg ctggcgcgga cggcattgtt ttttgatgtt
    22921 cgaaacccgc ttctggtgcc tggaaagagg ccgtcatata aaaaatcggt ttgccgtttt
    22981 gaatagcagc aacccggcgg gcgctgaagc tgttaccgtc acgcagcgtt tcgacatcat
    23041 aaataatcgg cttcttacta tcgccagggc gaagaaagta gctgtgaaac gaatgtacca
    23101 gccgctcttc agggacggtc tcttttgcag catacaaggc ctgacccacg acctggccgc
    23161 caaacacctg gcgtaaacct aaatcttcac tctggccgcg aaagagtcct tcctcaattt
    23221 tttctagatt taacaatgtc agtaaatttt ttagcgcctg actcatataa ctctccagta
    23281 acaaagctgc cgcagcaagc caaagtgagt tgagtataac gcaaatttgc cactggtccg
    23341 atgggtgcaa tggtctgaat tacgggctaa ttacaggcag aaatgcgtga tgtgtgccac
    23401 acttgttgac gttactattt tgttaaccac tcttccggcg aggaaagtta gcccgctggt
    23461 gcattgataa taaggagaaa tgaatgaaac tcgtgcacat ggccagtggt ttagcggttg
    23521 cgattgcatt ggcggcttgc gcagataaaa gcgcggatat tcagacgcca gccccggctg
    23581 caaatacgtc tatttcagca acacaacaac ctgctatcca gcaaccgaat gtctccggta
    23641 ccgtctggat ccgtcagaaa gtcgcactgc cgcctgatgc tgtgctgacc gtgacacttt
    23701 ctgacgcgtc gttagccgat gcaccgtcaa aagtgctggc gcagaaagcg gtgcgtaccg
    23761 aaggtaaaca gtcaccattc agctttgttc tgccatttaa cccggcagat gttcagccga
    23821 acgcgcgtat tctgttgagt gcggcgatta ccgtgaatga caaactggta tttatcaccg
    23881 ataccgttca gccggtgatc aaccagggcg gaactaaagc cgacctgaca ttggtgccgg
    23941 tacagcaaac cgccgtgccg gttcaggcca gcggtggcgc aacgactacc gtaccttcga
    24001 cttcaccaac tcaggtgaat ccgtcttcgg cagttccagc tcctacgcaa tattaagcga
    24061 cgttaaccct ctccgcccgg agagggttag tagttccagc gataacgctg caagtcgatt
    24121 tgcccgcttc ccgataccat tacaccttct gccagtaatg cctgtcgctg acgctgtaaa
    24181 tccggtccgg ttagcgaaat tgtgccgtgg cgattaacca cccggtgcca gggtaaggtg
    24241 ctgccttcag ggagacgctt taacacaccg cccacctggc gcgcggcgcg gggcgatccc
    24301 gccagtttcg ccacatcacc gtaagtggtg acatagcctt cgggaatagc ggcgacgatt
    24361 tgccagacgc gttggggaaa tgaatcttct ttttccatct ttttttcctg aggtaatttt
    24421 tcagcataat ctggaaaaac gcccgagtga agtcgcattg cgcaagaaac cagcatctgg
    24481 cacgcgatgg gttgcaatta gccggggcag cagtgataat gcgcctgcgc gttggttctc
    24541 aacgctctca atgggggctc tgttggttct cccgcaacgc tactctgttt accaggtcag
    24601 gtccggaagg aagcagccaa ggcagatgac gcgtgtgccg ggatgtagct ggcagggccc
    24661 ccacccattt ctgcctccca ccgtttcgtc aaaaaacccc aacatggcta aactttaacc
    24721 acaactgacg tcgcaagaat tgtctggctg cgcagtacgc ttcggaggta tgtctgatga
    24781 agtatgttga tggttttgtg gttgccgttc ctgccgataa aaaggatgcc tatcgggaaa
    24841 tggccgctaa ggctgcgcca ttgtttaaag agtttggcgc gcttcgtatt gtcgaatgct
    24901 gggccagcga tgtaccggat ggcaaagtga ccgattttcg tatggcggtg aaagcggaag
    24961 agaatgaaga ggttgtcttt agctggattg aatacccttc aaaagaggtc cgcgacgctg
    25021 ctaatcaaaa gatgatgtcg gacccacgga tgaaagagtt cggcgagtcc atgccgtttg
    25081 acggcaagcg aatgatctat ggcggattcg agtcaatcat cgacgaatag gtagcgtgac
    25141 gggctgtgcg gcgtgccgtc agcccaaaaa ttcacaaatg ttgctcggcc cagcgtaaga
    25201 aatcttcttt cggtaatgcc ttgctgtaga gccagccctg accgtagtgc acgccatgct
    25261 ggcgtagcca ctcttcttgt ttactggttt cgattccctc cgctaccatt ttcagtttca
    25321 gtgtttttgc catttcgatg atatgcggcg tgacattttt atattccagc gcatcaacga
    25381 aagatttatc gatcttcaga atgtcgacat ccagatcctg taaataactt aaacttgaat
    25441 atcccgtacc aaaatcatca agataaattt catggcccgc ctcccggtag cgagaaatta
    25501 tcggggcgct ggttttcgga tcggcaaact cgcgttcagt gagttcaagc gcgatctgtc
    25561 tgggattaac ctgatagtga ttgatcattt cacgcagcaa ttgcgggatt ttttccgagg
    25621 tgagcacggt ggattcaaga ttgatcgaaa tatgctgctg tggatgctga cgcagccagt
    25681 cgcccatatc ttcaaagacg cttcttataa tcagtagcgt caatggctca gaaaggcccg
    25741 tttgctgtgc cagcggaata aaactatctg gtgacaacca actaccgtct gtctgcggcc
    25801 agcgcgccag tgcctcagca ccgacaattt tgccattggc taaggagaca atcggctgat
    25861 agtgcacgca aatatcacga ttttcgatag catcctgcag ccgatgatgc ggtgactgga
    25921 tacggcgcaa aatacgcagc acaaacatcg ctgccagcag gccaatcacc aacccgagcg
    25981 gtaaccaaat aaagacttgc cgatgccagc ctttctgcaa cattttcgtt gaagcccagg
    26041 tgatgatcga aatattcatc tccgggaagg gcaggatatc gtaaatgatt ccattatttt
    26101 cgatatgctc accgggtgtt ttttgtagcc tggtaataat tccctgagca atttcatcgc
    26161 tgctggttat gacaacgtta tgggcattgc caataatggc ggcatcaatt tgccatgagc
    26221 tatagggaat gacatcaata aaggaagcgg ggtcgatcat gacgacataa tgtgccgttc
    26281 ccatggcgac catgtaacgg ataatgccta aatcgttatg cgatgttaac cagacacgat
    26341 aaccatcttt cgaaatttta cctggctcgg ggaaggtatc gggcggactt tcatgctcca
    26401 gagacgaaca ctggggaacg ttgttatcga tataaaccac ttcctgaata tagcgataac
    26461 tgtaagatac ccgacgcatt tccatgagat gggcttcgct acaggcagcg ccttgccatc
    26521 tttccagctc ttgcagcgca tctttccctt gtgtcgccac cttattggcc cgaatagcga
    26581 cgcgggagga ataggtatcc agctcttcaa taaacgatgt ttctacctgc tgatgggcca
    26641 gccagatgct taagccgaca ggcagcaata ctgaaagaat cagtactccc gaaataaggc
    26701 cgaccagatg tcgtgttctc accagcatat ccttattaac acagtgtagg gaaagtatat
    26761 ctgattgtgg aaaaaggctg gcaggagata aggaggtaga gctggtattt catgaagtga
    26821 tgggtggaaa cgatcattgt catttgacca ggcgcagagc tttacgcctg gtatgtaacc
    26881 ctaacttaag atgttgattc ggcgcgagcg agggtaaaaa tatcgtaaaa agacagttca
    26941 cctttacgga cgatcatttt ttgcagttgt tccttatgaa catccgcgac acgcggagag
    27001 ttcaagatgg cttcaaccag cgcggcaggt acaaagcgcg tgttgtacca ttcgccgtta
    27061 tagcaaacgc ggaaatccag cacgtcgata tcttcatagc ggtagcgtgg gtagacatcg
    27121 aaaaagaaga aaccattaag caggataaat aatacaacca gtagccagac agagcctgaa
    27181 agcgtttcgg actgcaacat caccgccagg gtggcaaaaa aagcaacgta cataccgata
    27241 aacagccccg gatgtttacg gataaaactg atactaaagc ggggtttgtt gtcgcgtttt
    27301 tcgcgggtat tcagagactc aatcgtttcg gtcagcaggc gttgtatttc ggtcatcgtt
    27361 tgccttcgtg gtatctgcaa ctttccaggg aacaccctat tttaacgtgg gaatagtaga
    27421 ggggaagtaa cagatttacc acaattttgc ataacagttg cgaaaaaccg attacaattt
    27481 tttaattatt ctggctggat taccgcccac gacaacgttg ttcgggacat cttttgtgac
    27541 aactgcacct gaggctacca cgacgttatc accaatggtc acaccagggt taatgaccgc
    27601 gcgtccgcca atccagacgt tattaccgat ggtgacgggt ttccccagtt cagcaccgct
    27661 attacgtgct acagggtcga tgggatgtgt tgccgtgtag atatgaacgc ctggtgccaa
    27721 catacagtta tcaccgatgc gaatagggca gacatcaagc atcacgcaat cgaagttggc
    27781 gaaaaaatta ttaccgagaa aaatgttata gccatagtca cagcgaaacg ttggctcaat
    27841 ataagcctct gtcacctgac cgaatagatc agcgagaatt tgctggcgta atgtgtgctc
    27901 ttccgccagg gaatgattgt atcggtgaat aagctgacga gcgcgcaggc gatcgcgaga
    27961 taacgtctca tctgccgagc gatacaactc accagcaatc atcttttctt tttctgtgct
    28021 cattactgac ctccggttgc tgaaaatgcg gcaacacagt aagacgaaaa gtcagtgaag
    28081 agaacgttcc cattacatct gtgtgataaa gatcacatag ggtatgtctt catggcgaaa
    28141 aagtataaaa ttcttaataa acagccggtt atagctccga aagcgaatta tttattagcg
    28201 aataaatttc caaactgagg aagggatctt gtcgtacagt ttattcatgg tcaattcggc
    28261 gaggcggtga tctgcggctg agtaaaatac cgccagttca ttatctgata attcgtattt
    28321 atttttctcg ataacacgct ccagcgtgtc aattgtctgg caacgacgta aacgcattaa
    28381 ataatcggtt ttcgttaaag gtttttcgga catacttcta cccatggttg taataattct
    28441 aacaagataa actcgcagga ttctctttcg tcgcattgac aaaacaacgg aatagtcgat
    28501 tacctgactt ccgccatttc tgaagatcct gcatattaat accataacta ctgaacaaca
    28561 taaaggtgtc atccagatat tcgtcgatct gctcaatgag cttattgtct tcattatact
    28621 taattttgta attaagtgcg aaggtcgcaa tatgctcaat cagttcattc aactggaggt
    28681 tgatcgccga ggttgggtcg ttaacccagc catgattgct ttcttcaagg tttgcaaggc
    28741 agtcatgata cagggtttca cagagaaact taagctgtgc gatatcatgt cttttgggtg
    28801 agtattcatc cataacgcgt ccccttctta gcggttgaac taacggacac ctttcgggat
    28861 ggaaaaaact tactgacctg gacttgccct ctcgtttgtt agcttaacta taagccactc
    28921 tttgcaggtt ttcatcgcat tattacgaaa aattacaatt aggaaggaat cgggacagga
    28981 ctcacttttt catactagca ctaacccgca gcaggtaaaa gcagtttgct cgaactgttc
    29041 ataacatttc aggattatcg ccagatgaaa ttggtgacac taataccagg attgctctga
    29101 atatgacgta ataaccgagg aatgaataaa gaattaccgc aaataattaa gaatagcctc
    29161 taaatgatta tggattatta aatctattag cgctacttaa tataaattaa ctaacaatca
    29221 gtatttttta tgaattttat ccgtggttaa tactggtttt cgtatgagat cctgagttgg
    29281 tggttcaatt actccttaat gttcgtaggt tatgcataaa aaaggccgct tgcgcggcct
    29341 tagtgattac acgttgtatc aatgatgatc gacagtatgg ctgtgctcga tatcttcatt
    29401 cttgcggcta aagcggcggc gaaccacaac aaagaatacc ggaacgaaga agattgccag
    29461 taccgttgcg gtcaccatcc cgcccattac accggtacct actgcgttct gcgcgccgga
    29521 accagcacca gtactgataa ccagcggcat aacgccgagg ataaacgcca gcgaggtcat
    29581 caggatcgga cgtaaacgca tccgcactgc atcaagcgtc gcttcaatca gacctttacc
    29641 ttctttatcc atcaagtctt tggcgaattc gacgataagt atcgcgttct tcgccgacaa
    29701 cccaatggtt gtgagcaggc ctacctggaa gtaaacgtca ttggtcaggc cacggaaggt
    29761 ggcagccagc aacgcaccga taacccccag cggaacgacc agcataacgg agaacggaat
    29821 cgaccagctc tcgtacagcg ccgccagaca caggaatacg acaatcaacg aaatcgcgta
    29881 cagtgaaggt gcctggttgc cggagaggcg ttcctgatag gacatccccg tccagtcata
    29941 gccaacaccg gtaggcagtt tgctcgccag ttgttccatt agctccattg cttcaccggt
    30001 acttttaccc ggtgccgcct ggcctaagat ttccatggat ggcaggccat tgtaacgttc
    30061 cagacgcggc gaaccgtact cccaacgaga agaggagaat gccgagaacg gcaccatctg
    30121 accatcagca gcacgaacat accagtcgcc gatatcatcc ggcagcatac ggtatttcgc
    30181 ttctgacatg acgtaaactt tcttcacacg accgcggtcg ataaagtcgt tcacatagct
    30241 accgccccat gcagcgccca gagtggtgtt aatgtcgttg atagaaacac ccagcgcctg
    30301 cgctttttcc tggtcgatat caatcttaaa ctgcggggta tcttccagac cgtttggacg
    30361 tacgctggtc agcatatcag ggtgcttcgc tgcttctgca agcagctggt tacgcgcctg
    30421 agtcagtttt tcgtgaccaa ggccagcctg gtcaatcagc tcaaagtcaa agccggttgc
    30481 agtacccagt tccacgattg cgggcaggtt aaaggcgaaa accatcgcat ctttgatttg
    30541 cgagaaagcg cgtgttgcac gcatggtaat cgcttcaact ttgttttctt cgcccggacg
    30601 atcggcccag tccttcaagg aaacgaacgc aataccggta ttctgaccac gtcccgcaaa
    30661 gccgaagccg ttaacggcga acaccgactc aacgttgttc ttttctttgg tcagatagta
    30721 atgcgttacc tcattgagca ctttctgcgt acgttcctgc gttgcacctg ctggcagctg
    30781 aaccatggtc ataaatacgc cctggtcctc atctggcaag aaggagcttg gcagacgcac
    30841 gaacagatag gccatgccga ccacgatgat cagatacagc accaggtaac gccccgtact
    30901 gcgcagaata ccgcctacgc tgtcggtgta gtggtgcgtg ctcttctcga acatgcggtt
    30961 aaaccagccg aagaagcctt ttttaccttc cccgtgatcg cctttggcaa tcggtttcag
    31021 catggtggca caaagagccg gagtcaggat caacgctacc agtaccgaca gcgccattgc
    31081 tgaaacaatg gtaatagaga actgacgata gatagcacca gtagaaccgc caaagaaggc
    31141 catcggtacg aataccgccg acagtaccat cgcgataccg accagagcgc cctgaatctg
    31201 ccccatcgac ttacgggtag cttcttttgg cggcaaacct tcttccgcca taacacgctc
    31261 cacgttttct accacaacga tagcgtcatc caccaacaag ccgatagcga gcaccatccc
    31321 gaacattgtt agcgtgttta tcgagaagcc aaaggcggca aggacggcaa aggtcccgag
    31381 caataccacc ggtacggcaa tggtcggaat caacgtcgcg cggaagttct gcaggaacag
    31441 atacataacc aggaacacga ggatgatcgc ttcgaccagc gttttaacca cttcgtgaat
    31501 agagattttc acgaacggcg tggtgtcgta cgggtaaaca attttcagac ccgacgggaa
    31561 gaacggttcc atcttcgcca gttcagcacg gattgccgca gcggtatcca gcgcgtttgc
    31621 accggtcgcc agcttgatcc ccagaccgga agccggttgg ccgttaaact ctgcgatgat
    31681 gtcgtagttc tcaccaccca gctcaatctt cgccacatca cgcagcagta cgcgggaacc
    31741 atcttgattc actttcagca ggattttgcc gaactcttca gtagaggtca gacgcgtctg
    31801 agcaataata gaggcgttaa gctgttggcc tttcaccggc ggcgtaccac cgagctgacc
    31861 cgccgcaacc tgggcgttct gcgctttgat ggcggtaatg acatcaaccg gcgttagctg
    31921 gaatttgttc agctcattcg ggttcatcca gatacgcatc gcgtactgtg aaccgaacaa
    31981 ctgaacatca cccacgcccg acgtacggct gatggcatct ttcatattcg ccgctacgta
    32041 gtcagagata tcctcctgcg tcatggtgcc atcggtgttg ataacgccga caaccatcag
    32101 gaagctgctg gatgatttct caacgctcac cccttgctgc tgaacttctt gcggcagcaa
    32161 cggcatcgcc agctgcagtt tgttctgcac ctgaacctgc gcgatgtccg catcagtacc
    32221 agactcaaag gtcagggtga tctgcacggt acccgtggag tcactgttag aggacatgta
    32281 catcaggtta tcgataccgt tcatattctg ttcgataacc tgtgtcaccg tgtcctgcac
    32341 tgttttcgca tcagcgccgg ggtaggaggc ggagatcgtt actgccggcg gtgcaatcgt
    32401 aggatattgc gccaccggca gtttgaggat cgccagcccc cctgccaaca tgatgataat
    32461 ggcgatcacc cacgcaaaaa tcgggcgatc gataaagaaa ttaggcatgt cttaacggct
    32521 cctgtttaag ttaagacttg gactgttcag gctgagcacc gcttgcggct tgctggttat
    32581 tatcagcggt aacttcttgt gcttttacct ggacaccagg acgcactttc tgcagcccac
    32641 ttattactac gcgatcgcct gctttcagac cttctgtcac cagccactta tcgccaatag
    32701 cctggcttgc aacgatcgga cgggtttcca ctttgtcatc cgcgccaact accagtacgg
    32761 tggcatcacc acgcggcgta cgggttacgc cctgttgcgg gactaaaata gcgtttggat
    32821 taagcccttc ttccagacgt gcgcgcacga acatacccgg cagcagagtg tgatccgggt
    32881 tcgggaagat agcgcgtagg gtgatagacc cagtggtctg atcaacggta acgtcagaga
    32941 attccagcgt accgtcctgc gggaacttaa tgccgtcact ggtgatcagc gacactttgg
    33001 ctttgccgtt ctcttgtttc agcgtgccat tcgccagttc ctgtttcagg cgcaggaagt
    33061 cgttgctgga ctgggtcaca tcaacgtaga tcggatcaag ttgctgcacg gttgccagcg
    33121 cagtcgcctg accgttctgt accaatgcgc cttccgtcac gttcgactta ccaatgcgac
    33181 cgctaatcgg agaggtgact ttggtgtaag ccagattgat ccgcgcagtt tcaacggcag
    33241 ctttcgccgc agttaccgca gcattcgcct gttgcgcatc agccagagcc tgatcgtact
    33301 cttgcttact gatgtactga gtaccgagca gtttctgata acgattcacc gtcaattgcg
    33361 cgatattggc tgcagcctgg gctttcgcca gatcaccttt cgcactgtcg tatgtcgcct
    33421 gataggtcgc aggatcaatc tgatagagag agacaccagc ttcgatgtcg ctaccttctt
    33481 tgaaattacg cttcaggata atcccgctaa cttgaggacg aacttctgcg atccggtagg
    33541 cactggtgcg acccggaagc tcggttgtga tctgcagagg ttcagttttg actgttacta
    33601 cgccaacggc gggcatctgc tggccacctt gttgggcctg tttgtcgtca catcctgtta
    33661 gggctaagct gcctgagagc atcagaacga ccgccagagg cgtaaaccct ctgtttttgt
    33721 tcatatgtaa acctcgagtg tccgatttca aattggtcaa tggtcaaaag ttaataaacc
    33781 cattgctgcg tttatattat cgtcgtgcta tggtacatac attcacaaat gtatgtaaat
    33841 ctaacgcctg taaattcacg aacatatggc acgaaaaacc aaacaagaag cgcaagaaac
    33901 gcgccaacac atcctcgatg tggctctacg tcttttctca cagcaggggg tatcatccac
    33961 ctcgctgggc gagattgcaa aagcagctgg cgttacgcgc ggtgcaatct actggcattt
    34021 taaagacaag tcggatttgt tcagtgagat ctgggaactg tcagaatcca atattggtga
    34081 actagagctt gagtatcagg caaaattccc tggcgatcca ctctcagtat taagagagat
    34141 attaattcat gttcttgaat ccacggtgac agaagaacga cgtcgattat tgatggagat
    34201 tatattccac aaatgcgaat ttgtcggaga aatggctgtt gtgcaacagg cacaacgtaa
    34261 tctctgtctg gaaagttatg accgtataga acaaacgtta aaacattgta ttgaagcgaa
    34321 aatgttgcct gcggatttaa tgacgcgtcg ggcagcaatt attatgcgcg gctatatttc
    34381 cggcctgatg gaaaactggc tctttgcccc gcaatctttt gatcttaaaa aagaagcccg
    34441 cgattacgtc gccatcttac tggagatgta tctcctgtgc cccacgcttc gtaatcctgc
    34501 cactaacgaa taaccctgaa tctgactcca ggatttttcc tggacatttt cgtcgttgct
    34561 attctggttc actgcgtcgt gatattcttg cggtttgact ttttcaggtc gttcttcagg
    34621 ttcagaaacc ttcattcatc atgactatgt tccagtatta caaacgatca cggcattttg
    34681 ttttttcagc atttattgct tttgtttttg tcttgttatg ccagaacacg gcgtttgcgc
    34741 gggcgtcatc gaatggtgat ctgccgacaa aagcggacct gcaggcgcaa cttgactcac
    34801 taaataaaca aaaagatctt tctgctcagg acaaactggt gcagcaggat ctgacagata
    34861 cattagccac cctcgataaa atcgatcgca taaaagaaga gacagttcag ctacggcaaa
    34921 aagtcgctga agcgccggaa aaaatgcgcc aggcgaccgc ggcgttaaca gcacttagcg
    34981 atgtcgataa cgacgaagaa acgcgcaaaa ttctgagtac gctttcgttg cgccagctgg
    35041 aaactcgcgt tgcccaggcg ctggacgatt tgcaaaacgc acaaaacgat ctggcgtctt
    35101 ataacagcca gctggtttcg ttacagacgc agcccgaacg cgtgcaaaat gcgatgtata
    35161 acgcttcgca gcagctgcaa caaattcgca gtcgtctgga tgggactgat gtcggcgaga
    35221 ctgccttacg tcccagccag aaagtgttaa tgcaggctca gcaggcgttg ctgaatgcgg
    35281 agattgacca gcagcgtaaa agcctggaag ggaacaccgt cttgcaggat accttgcaaa
    35341 agcaacgtga ttacgtgacg gcgaacagcg cccgtctgga gcaccagttg cagctgttgc
    35401 aagaagcggt aaacagcaag cgcctgactt taactgaaaa aacggcgcag gaagccgtct
    35461 ccccggatga agccgcgcgt attcaggcta atccgctggt gaaacaggaa ctggaaatta
    35521 accagcagtt aagtcagcgt ctgattaccg cgactgaaaa cggtaatcag ttgatgcagc
    35581 aaaacattaa agtcaaaaac tggctggagc gggcgctgca atcggaacgc aatattaaag
    35641 agcagattgc cgtcctgaag ggcagcctgc tgttgtctcg tatcctttac cagcaacaac
    35701 aaacgctgcc ctcggcggat gaactggaaa acatgaccaa ccgcatcgcg gatttgcgtc
    35761 tcgaacagtt tgaagttaac cagcagcgtg atgcactctt ccagagcgat gcgttcgtca
    35821 acaaactgga agaaggtcac accaacgaag tcaacagcga agttcacgat gcgttattgc
    35881 aagtggttga tatgcgtcgc gaattgctgg atcaactcaa caaacagttg ggtaaccagc
    35941 tgatgatggc cattaacctg caaatcaacc agcagcagtt aatgagcgta tcgaaaaacc
    36001 tgaaatccat cctgactcag caaatctttt gggtgaacag taaccgtcca atggactggg
    36061 actggatcaa agcgttcccg caaagcctga aagatgaatt taagtcgatg aaaatcacgg
    36121 tgaactggga aaaagcctgg cctgccgttt ttatcgcttt cctcgccggt ttgccgctgc
    36181 tgttgattgc cgggctgatc cactggcgtc tgggctggct gaaagcgtat caacagaaac
    36241 tggcttccgc tgtgggttcc ctgcgtaacg atagccagct caacacacca aaagcgatcc
    36301 ttatcgacct gatccgcgcg ctgccggtgt gcctgattat tctcgcggtt ggcctgattc
    36361 tgttgaccat gcagctcaac atcagcgaac tgctatggtc gttcagtaaa aaactggcga
    36421 ttttctggct ggtgtttggc ctgtgctgga aggtactgga gaaaaatggc gttgccgtgc
    36481 gtcacttcgg catgccggag cagcagacca gccactggcg tcggcaaatt gtccgcatca
    36541 gtctcgcgtt gctgcctatc catttctggt ctgtggtggc agaactttcc ccgctgcatc
    36601 tgatggatga tgtgctgggg caggcgatga ttttcttcaa cctgctgctg attgccttcc
    36661 tggtatggcc aatgtgccgc gaaagctggc gtgataaaga gtcgcacacc atgcgactgg
    36721 tcaccattac cgtgctgtcg attatcccga ttgcattgat ggtgctgact gccacgggtt
    36781 atttctacac cacgctgcgc ctctccgggc gttggattga aaccgtttat ctggtgatca
    36841 tctggaactt gctctaccag acggtattgc gcggcttaag cgtagcggcg cggcgtatcg
    36901 cctggcgtcg tgcgctggcg cgtcggcaga atctggtgaa agagggcgca gaaggtgccg
    36961 aaccgccgga agaacccacc attgcactgg aacaggttaa ccagcagacg ctgcgtatta
    37021 ccatgttgct gatgtttgcg ctgttcggtg tcatgttctg ggcaatttgg tccgatttga
    37081 tcaccgtgtt cagctatctc gacagcatca cgctctggca ttacaacggc actgaagctg
    37141 gcgctgcggt ggtgaaaaac gtcaccatgg gcagtctgtt gtttgcgatt atcgcctcaa
    37201 tggtggcctg ggcgttgatt cgcaacctgc ctggtttact ggaagtgctg gtgctctcgc
    37261 gactgaatat gcgtcagggc gcgtcgtatg ccattactac catccttaac tacatcatta
    37321 ttgccgttgg tgcgatgacg gtgttcggat cgctgggcgt ctcttgggat aaactccagt
    37381 ggctggccgc agcattatcc gtaggtcttg gttttggttt acaagaaatt ttcggtaact
    37441 tcgtctccgg tttgatcatt ctgttcgaac gtccggtgcg tattggcgat acggtaacca
    37501 ttggtagctt ctcggggacg gtaagtaaga tccgtattcg tgcgacaacg attaccgatt
    37561 tcgatcgcaa agaagtgatc atcccgaaca aagcgtttgt taccgagcgt ctgatcaact
    37621 ggtcgttgac tgacactact acgcgtctgg tgatccgtct cggcgtggcc tatggctccg
    37681 atctggaaaa agtgcgtaaa gtgttactga aggcggcgac tgagcaccca agggtgatgc
    37741 acgaaccaat gccggaagtc ttctttacgg cgtttggtgc cagcacgttg gatcatgagc
    37801 tgcgtctgta tgtgcgtgaa ctgcgtgacc gtagtcgtac tgtcgatgag ctgaaccgta
    37861 ctatcgatca gctgtgccgt gaaaacgaca tcaacattgc ctttaaccag cttgaagtgc
    37921 atctgcacaa cgagaagggc gatgaggtga cggaagtcaa acgcgactac aaaggcgatg
    37981 acccgacgcc agcggtaggg taataaccaa agggggcgac atttagttgc cccgagatta
    38041 ctgacaaagt gcgcgttgtt catgccggat gcggcgtgac cgccttatcc ggcctacgaa
    38101 accgcaagaa ttcaatgtat tgcaggagcg gtgtaggcct gataagcgta gcgcatcagg
    38161 cagttttgcg tttgcccaca atcttagggg acatttagcg gccccattta tttctcactt
    38221 ttccgcctca tcatcgcgcg ttaatttctt ttcataatcg cgttttacaa tatccagcgc
    38281 gcgcagcacg gtgcgggctg ggatctgatt ttcttccagc agcacaatca aatcgacagc
    38341 cagtttgaca tcgtcagggg cattttccag tgacatactc tctccattgc tagcgggtta
    38401 agcgcgctag cctgttttcg attttttcca gcgcatggcg gcagcgtgcc aggcgcgctt
    38461 cataggcttc cacttcacga tgcagcgttt gctgttccac gagatcggtc acccgcgcca
    38521 ggcgggctct gcgttcggca accatctcac gtagccgccg ctcaaaatcc tgatgctgaa
    38581 tacgtttacg ctgccagcgg gcaattttcg gtggcgcact gtcccactcg cgcaatgacc
    38641 aggcggaggc ttcacgcgcg atggcttcca gttgtgccgc cagatgttcc gccagccagg
    38701 ccacttgcgg cagctgttgc tgctcaactg catgacgaag cgcagccaga ttatcgcccg
    38761 cctcatcgag acaagcctgc agtgttgtcg cacgggtttg aaaaaggtgt ctgtcaaaac
    38821 gggcacttag cgtggcaaat tgcgctaccg gggcacaacg ctgacgcagc gtagcgagct
    38881 gtccttccag tttttccagc agcagggcgg ttttcacgat aagacctcaa tgaaaatgat
    38941 aattgttatg ctaaagtagc cactctgtaa gctgacaaca ctcaatatgc aacgaatcat
    39001 tttaatcatc attggctggc tggcggtagt gctgggtacg ctgggcgtgg tattaccggt
    39061 attaccgacg acgccgttta tcctgctggc ggcctggtgc tttgcccgtt cttccccgcg
    39121 ctttcacgcc tggttgctgt accgctcatg gtttggcagc tatctacgtt tctggcagaa
    39181 acatcatgcg atgccgcgcg gcgtcaaacc gcgggcgatt ttgcttattt tgctcacgtt
    39241 tgccatttct ctgtggttcg tccagatgcc atgggtgcgc atcatgttgc tggtaattct
    39301 cgcctgtttg cttttctata tgtggcgaat tccggtgatt gatgaaaagc aagaaaagca
    39361 ctgaagcaca acaatcgcag ttgcaattat tgcgtacagc cagtacattc tggcgttttc
    39421 gagcacaggc gcaggcggtc aaaggttaaa caactgttac ttttgatacg tttaaaacgc
    39481 gccgtgagta ccaccgtaac aagcaggcat acacttatga ccgcgactgc acagcagctt
    39541 gagtatctca aaaatagcat caaaagcatt caggactacc caaaacccgg cattcttttc
    39601 cgcgatgtca ccagcttact ggaagacccg aaagcttacg ctctcagcat cgacttgctg
    39661 gttgagcgtt acaaaaatgc gggcattacc aaagttgtcg gcaccgaagc gcgtggcttc
    39721 ttgtttggcg ctccggtagc tctgggtctg ggcgttggct ttgtaccggt ccgtaaaccg
    39781 ggcaaactgc cgcgtgaaac catcagtgaa acttacgacc tggaatacgg caccgatcag
    39841 ctggagatcc acgttgatgc catcaaaccg ggcgacaaag ttctggtggt ggacgatctg
    39901 ctggcaaccg gcggcactat cgaagcgacc gttaaactga tccgtcgtct gggtggtgaa
    39961 gtggctgacg ctgcgttcat tatcaacctg ttcgatctcg gcggcgaaca gcgtctcgaa
    40021 aaacagggca ttaccagcta cagccttgtc ccgttcccgg gccattaatt atcgccagtc
    40081 ttgtgctgcc cacgctacgg acagcacaag atgtgcattc agcctcgccg ttctgacggg
    40141 gctgtgttag cattacccct tcgtgaatcc accttccagc gtttcagagc ctgccaatga
    40201 gttatcaggt cttagcccga aaatggcgcc cacaaacctt tgctgacgtc gtcggccagg
    40261 aacatgtgct gaccgcactg gcgaacggct tgtcgttagg gcgtattcat catgcttatc
    40321 ttttttccgg tacccgtggc gtcggaaaaa cctctatcgc ccgactgctg gcgaaggggc
    40381 taaactgcga aaccggcatt accgcgacgc cgtgcggcgt gtgcgataac tgtcgtgaaa
    40441 tcgagcaggg gcgctttgtc gatctgattg aaatcgacgc cgcctcgcgc accaaagttg
    40501 aagatacccg cgacctgctg gataacgtcc agtacgctcc ggcgcgtggt cgtttcaaag
    40561 tttatctgat cgacgaagtg catatgctgt cgcgccacag ctttaacgca ctgttaaaaa
    40621 cccttgaaga gccgccggag cacgttaagt ttctgctggc gacgaccgat ccacagaaat
    40681 tgccggtgac gattttgtcg cgctgtttgc aatttcatct caaggcgctg gatgtcgagc
    40741 aaattcgcca tcaacttgag cacatcctca acgaagaaca tatcgctcac gagccgcggg
    40801 cgttgcaatt gctggcgcgc gccgctgaag gcagcctgcg agatgcctta agtctgaccg
    40861 accaggcgat tgccagcggt gacggccagg tttcaaccca ggcagtcagt gcgatgctgg
    40921 gtacgcttga cgacgatcag gcgctgtcgc tggttgaagc gatggtcgag gccaacggcg
    40981 agcgcgtaat ggcgctgatt aatgaagccg ctgcccgtgg tatcgagtgg gaagcgttgc
    41041 tggtggaaat gctcggcctg ttgcatcgta ttgcgatggt acaactttcg cctgctgcac
    41101 ttggcaacga catggccgcc attgagctgc ggatgcgtga actggcgcgc accataccgc
    41161 cgacggatat tcagctttac tatcagacgc tgttgattgg tcgcaaagaa ttaccgtatg
    41221 cgccggaccg ccgcatgggc gttgagatga cgctgctgcg cgcgctggcg ttccatccac
    41281 gtatgcctct gcctgagcca gaagtgccac gccagtcctt tgcgcccgtc gcgccaacgg
    41341 cagtaatgac gccaacccag gtgccgccgc aaccgcaatc agcgccgcag caggcaccga
    41401 ctgtaccgct cccggaaacc accagccagg tgctggcggc gcgccagcag ttgcagcgcg
    41461 tgcagggagc aaccaaagca aaaaagagtg aaccggcagc cgctacccgc gcgcggccgg
    41521 tgaataacgc tgcgctggaa agactggctt cggtcaccga tcgcgttcag gcgcgtccgg
    41581 tgccatcggc gctggaaaaa gcgccagcca aaaaagaagc gtatcgctgg aaggctacca
    41641 ctccagtgat gcagcaaaaa gaagtggtcg ccacgccgaa ggcgctgaaa aaagcgctgg
    41701 aacatgaaaa aacgccggaa ctggcggcga agctggcggc agaagccatt gagcgcgacg
    41761 cgtgggcggc acaggtgagt caactttcgc taccaaaact ggtcgaacag gtggccttaa
    41821 atgcctggaa agaggagagc gacaacgcag tatgtctgca tttgcgctcc tctcagcggc
    41881 atttgaacaa ccgcggtgca cagcaaaaac tggctgaagc gttgagcacg ttaaaaggtt
    41941 caacggttga actgactatc gttgaagatg ataatcccgc ggtgcgtacg ccgctggagt
    42001 ggcgtcaggc gatatacgaa gaaaaacttg cgcaggcgcg cgagtccatt attgcggata
    42061 ataatattca gaccctgcgt cggttcttcg atgcggagct ggatgaagaa agtatccgcc
    42121 ccatttgatc gtaagcacag cttacgttcg tcatccttaa cgtgattgag agagaaacct
    42181 atgtttggta aaggcggtct gggtaacctg atgaagcaag cccagcagat gcaagaaaaa
    42241 atgcagaaaa tgcaggaaga gatcgcgcag ctggaagtca ccggcgaatc tggcgcaggt
    42301 ctggtaaaag tgaccatcaa cggtgcacac aactgccgtc gcgtagagat cgacccgagc
    42361 ctgctggaag acgacaaaga gatgctggaa gacctggtgg ctgcagcatt taacgacgca
    42421 gcacgtcgta ttgaagaaac gcagaaagaa aaaatggcct ctgtatcctc cggaatgcag
    42481 ctgccgcctg gctttaagat gccgttctga tgcaaaccag cccgctgtta acacagctta
    42541 tggaagcact gcgctgtctg ccgggcgttg gcccgaagtc ggcgcagcgt atggcgttca
    42601 cgctgcttca gcgcgatcgt agcggcggga tgcgtctggc gcaggcgctc acccgggcga
    42661 tgtcggaaat cggccactgc gccgattgcc gcactttcac cgaacaggaa gtctgtaaca
    42721 tctgttcgaa tccgcgtcgt caggaaaacg gtcaaatctg tgtggtggag agtccggcgg
    42781 acatctacgc cattgagcag acggggcagt tttcaggccg ttattttgtg ttgatggggc
    42841 acctgtcacc gctggacggc atcggtccgg atgatatcgg ccttgatcgt ctggaacagc
    42901 gtctggcaga ggaaaaaatc actgaagtga tcctcgccac caatcccacg gttgaaggtg
    42961 aagctaccgc taactacatt gccgagcttt gcgcgcaata tgacgtggaa gccagccgaa
    43021 tcgctcatgg cgtaccggtt ggcggcgaac tggaaatggt cgatggcacc acgctgtcac
    43081 actcccttgc cgggcgtcat aagattcgtt tttaagcaaa cgagagcagg atcacctgct
    43141 ctcgcttgaa attattctcc cttgtcccca tctctccctc atcctgtttt taaccttaaa
    43201 atggcattat tgaggtagac ctacatgaaa ggacaagaaa ctcgtggttt tcagtcagaa
    43261 gtgaaacagc ttctgcacct gatgatccat tctctctatt ccaataaaga aatcttcctg
    43321 cgtgagctta tctctaacgc ctccgatgcg gcggacaagc tgcgtttccg tgcgctatct
    43381 aacccggacc tgtacgaagg tgatggcgaa ctgcgcgttc gtgtctcttt cgataaagac
    43441 aagcgcacgc tgaccatctc cgataacggc gtggggatga cccgcgacga agtgattgac
    43501 catctgggga ctatcgctaa atccggtacc aaatcattcc tcgaatccct gggttctgac
    43561 caggcgaaag acagccagct gatcggtcag tttggtgttg gtttctactc tgcgtttatc
    43621 gtggccgaca aagtgaccgt gcgtactcgc gcggcaggcg aaaaaccaga aaacggcgtc
    43681 ttctgggaat cggctggcga aggtgaatat accgttgccg acatcaccaa agaagatcgt
    43741 ggtactgaaa tcaccctgca tctgcgtgaa ggcgaagacg agttcctcga tgactggcgc
    43801 gtgcgttcca tcatcagcaa atactccgac catatcgcgc tgccggtaga gatcgaaaaa
    43861 cgcgaagaga aagacggcga aaccgttatc tcctgggaga aaatcaacaa agcgcaggcg
    43921 ctgtggactc gtaacaagtc ggaaatcacc gatgaagagt acaaagagtt ctacaaacac
    43981 atcgcccacg actttaatga tccgctgacc tggagccaca accgcgttga aggtaagcag
    44041 gagtacacca gcctgctgta catcccgtcc caggctccgt gggatatgtg gaaccgtgat
    44101 cataaacacg gcctgaaact gtacgtccag cgtgtgttca tcatggacga cgcagaacag
    44161 ttcatgccga actatctgcg cttcgtgcgt ggtctgattg actccagcga tctgccgctg
    44221 aacgtttccc gtgaaatcct ccaggacagc acggtaacgc gtaacctgcg caatgcgctg
    44281 accaagcgtg tgctgcaaat gctggaaaaa ctggcgaaag acgacgcgga aaaataccag
    44341 accttctggc aacagtttgg cctggtactg aaagaaggtc cggcggaaga tttcgctaac
    44401 caggaagcga tcgccaaact gctgcgtttt gcttctactt ataccgattc ttctgcgcag
    44461 accgtgtctc tggaagacta cgtttcccgc atgaaagaag ggcaggagaa aatctactac
    44521 atcactgcag acagctatgc ggcagcgaag agcagcccgc acctggaact gctgcgtaag
    44581 aaaggcatcg aagttctgct gctttccgac cgcatcgatg agtggatgat gaactatctg
    44641 actgagttcg acggtaaacc gttccagtcg gtgtctaaag ttgacgagtc gctggaaaaa
    44701 ctggctgacg aagttgatga gagcgcgaaa gaagcggaga aagcactgac tccgttcatc
    44761 gaccgtgtga aagccctgct cggcgagcgc gtgaaagatg tccgtctgac tcaccgtctg
    44821 accgatacgc cagcgatcgt ttcgaccgac gcggacgaaa tgagcactca gatggcgaaa
    44881 ctgttcgctg cggcgggcca gaaagtgcca gaagtgaaat acatcttcga actgaacccg
    44941 gatcacgtac tggtgaaacg tgcggcagat actgaagatg aagcgaagtt cagcgagtgg
    45001 gtagaactgc tgctggatca ggcgctgctg gcagaacgcg gcacgctgga agatccgaac
    45061 ctgtttattc gtcgtatgaa ccagctgctg gtttcctgat gtcatgccgg atgacctttg
    45121 tgtcatccgg catttttctt ttcatcatct gcactttccg caaattatct cgccattaac
    45181 cgtttcagcc tcaggtgcct ttcttgaggc aatcgcctgt tggtggtatc gtttatcgct
    45241 ttttcaaaaa attcgacaca ttttaagggg attttcgcaa tgcgtatcat tctgcttggc
    45301 gctccgggcg cggggaaagg gactcaggct cagttcatca tggagaaata tggtattccg
    45361 caaatctcca ctggcgatat gctgcgtgct gcggtcaaat ctggctccga gctgggtaaa
    45421 caagcaaaag acattatgga tgctggcaaa ctggtcaccg acgaactggt gatcgcgctg
    45481 gttaaagagc gcattgctca ggaagactgc cgtaatggtt tcctgttgga cggcttcccg
    45541 cgtaccattc cgcaggcaga cgcgatgaaa gaagcgggca tcaatgttga ttacgttctg
    45601 gaattcgacg taccggacga actgattgtt gatcgtatcg taggccgccg cgttcatgcg
    45661 ccgtctggtc gtgtttatca cgttaaattc aatccgccga aagtagaagg caaagacgac
    45721 gttaccggtg aagaactgac tacccgtaaa gacgatcagg aagaaaccgt gcgtaaacgt
    45781 ctggttgaat accatcagat gactgcaccg ctgatcggct actactccaa agaagcggaa
    45841 gcgggtaaca ccaaatacgc gaaagttgac ggcaccaagc cggttgctga agttcgcgct
    45901 gatctagaaa aaatcctcgg ctaattcgaa agcgcgcacg gacagtcccc tcgccccctc
    45961 ggggagaggg ttagggtgag gggaacaggc ccgcacaagc aaacttatca gcaatctcag
    46021 gccggatatt cattcggcct tttacaaaaa cctcaatctg cgctattcct ttttctgatt
    46081 tgacctctca cagcaattag ttcttcttcc tcacttttcc gctacaatta tcaacaagtt
    46141 gaatcgataa gaggcggtaa tgcgtcagac taaaaccggt atcctgctgg caaacctggg
    46201 tacgcccgat gcccccacac ctgaagcggt aaaacgctat ctgaaacaat ttttaagcga
    46261 cagacgcgtg gttgatacct cacggttgtt atggtggccg ttgctgcgcg gcgtgatttt
    46321 gccgctgcgc tcgccgcgtg tggcgaagct gtacgcctcg gtctggatgg aagacggctc
    46381 gccgctgatg gtttacagcc gccagcaaca gcaggcgttg gcacaacgtt taccggatac
    46441 gcccgtagcg ctgggcatga gctacggctc gccatcactg gaaagcgccg tcgatgaact
    46501 cctggcagag catgtagatc atattgtggt gctgccgctt tatccgcaat tctcctgttc
    46561 tacggtcggt gcggtatggg atgaactggc acgcattctg gcgcgcaaac gtagcattcc
    46621 ggggatatcg tttattcgtg attacgccga taaccacgat tacattaatg cactggcgaa
    46681 cagcgtacgc gcttcttttg ccaaacatgg cgaaccggat ctgctgctgc tctcttatca
    46741 tggcattccc cagcgttatg cagatgaagg cgatgattac ccgcaacgtt gccgcacaac
    46801 gactcgcgaa ctggcttccg cactggggat ggcaccggaa aaagtgatga tgacctttca
    46861 gtcgcgcttt ggccgggaac cctggctgat gccttatacc gacgaaacgc tgaaaatgct
    46921 cggagaaaaa ggcgtaggtc atatacaggt gatgtgcccg ggctttgctg cggattgtct
    46981 ggagacgctg gaagagattg ccgagcaaaa ccgtgaggtc ttcctcggtg ccggcgggaa
    47041 aaaatatgaa tatattccag cgcttaatgc cacgccggaa catatcgaaa tgatggctaa
    47101 tcttgttgcc gcgtatcgct aaagctgagc ggtaaagaac tgagcgccgt cgcgaagagc
    47161 ctcgtcggcg gttttcatca ttcgtgaata atgcaaaaag gcatgcagcg tacctgggta
    47221 gagtttgaac tcacagggct gctgatgcgc cgctaacgtc tggtaaagca gacggctgtc
    47281 atccagcagc ggatcgaact ccgccccggc aataaaacag ggcggaactt cgcgagtgag
    47341 atcattatta aacagacagt aatacggtga ctcgcggtcc gcgtcgttgc ttaaatacgc
    47401 ctcttcatac atctgcaaat cctgttgcgt taagccatcc cagacaccgc ccaacagacg
    47461 acgagtcacg gaatcccgta atccgtaaag cccataccac agcaaaacac ccgcaatttt
    47521 accgcaatcg atctgtttat cacgcaacca caacgcactg gcgagcgcca gcatggcacc
    47581 tgcggaatca ccggcaaagc caatgcggga catattgatt tgataatcct ccgcctgctg
    47641 gtggaaataa caacaagcag ccacaatttc ctctatcgct tgcggaaaac gcgcttcagg
    47701 tgaaaggggg taatcaatac caatcaccgt acattggctg tagcttgcca gcaggcgcat
    47761 gatgcgatcg tgggtatcga gattgccgag aataaaaccg cctccatgca aataaaatag
    47821 cgtcgccggg ctatctggct gcggacaaaa gagacgtgtt tccacctgcc catattttgt
    47881 tggaaccatg taagctctgg ttgccatttc tggagcgccc gcattccaga atcggcgctc
    47941 aagcgtgtaa tactgtcgtt gctcagcaat cgttcccgtt gcgggccagg acggtaaatc
    48001 cgactgaaga gtattcacaa cggtcttcat ttcagcagaa ataaggtcca gaacaggtag
    48061 tttgttttcc ggcttcatac aaaaactcct ttcaaattac gtcattgtaa ggaaccactg
    48121 ccatgaaaat gcgatcccgc ctgctgatat tgaaactggc tgcgtctcgc gcgctcccgt
    48181 cagattgtgt taacattcgc cgctcagtta accacccgta aaaacaacca tgaaatttcc
    48241 cggtaaacgt aaatccaaac attacttccc cgtaaatgca cgcgatccgc tgcttcagca
    48301 attccagcca gaaaacgaaa ccagcgctgc ctgggtagtg ggtatcgatc aaacgctggt
    48361 cgatattgaa gcgaaagtgg atgatgaatt cattgagcgt tatggattaa gcgccgggca
    48421 ttcactggtg attgaggatg atgtagccga agcgctttat caggaactaa aacagaaaaa
    48481 cctgattacc catcagtttg cgggtggcac cattggtaac accatgcata actactcggt
    48541 gctcgcggac gaccgttcgg tgctgctggg cgtcatgtgc agcaatattg aaattggcag
    48601 ctatgcctat cgttacctgt gtaacacctc cagccgtacc gatcttaact atctacaagg
    48661 cgtggatggc ccgattggtc gttgctttac gctgattggc gagtccgggg aacgtacctt
    48721 tgctatcagc cctggccaca tgaaccagct gcgggctgaa agtattccgg aagatgtgat
    48781 tgccggagcc tcggctctgg ttctcacctc ttatctggtg cgttgcaagc cgggtgaacc
    48841 catgccggaa gcaaccatga aagccattga gtacgcgaag aaatataacg taccggtggt
    48901 gctgacgctg ggaactaagt ttgtcattgc cgagaatccg cagtggtggc agcaattcct
    48961 caaagaccac gtctctatcc ttgcgatgaa cgaagatgaa gccgaagcgt tgaccggaga
    49021 aagcgatccg ttgttggcat ctgacaaggc gctggactgg gtagatctgg tgctgtgcac
    49081 cgccgggcca atcggcttgt atatggcggg ctttaccgaa gacgaagcga aacgtaaaac
    49141 ccagcatccg ctgctgccgg gcgctatagc ggaattcaac cagtatgagt ttagccgcgc
    49201 catgcgccac aaggattgcc agaatccgct gcgtgtatat tcgcacattg cgccgtacat
    49261 gggcgggccg gaaaaaatca tgaacactaa tggagcgggg gatggcgcat tggcagcgtt
    49321 gctgcatgac attaccgcca acagctacca tcgtagcaac gtaccaaact ccagcaaaca
    49381 taaattcacc tggttaactt attcatcgtt agcgcaggtg tgtaaatatg ctaaccgtgt
    49441 gagctatcag gtactgaacc agcattcacc tcgtttaacg cgcggcttgc cggagcgtga
    49501 agacagcctg gaagagtctt actgggatcg ttaagttatc gtcggttcgt aggccagata
    49561 aggcgttcac gccgcatctg gcatttggct ctcgatgccc gatgcgacgc tggcgcgtct
    49621 tatcatgcct acatattttt catattttac atccggcaac caccgtttac cccgtcacca
    49681 cttcacccgc cggtggcgtt tccagcagtt ccagcatagt acgggcaatt tcacgttcac
    49741 ccatcactac ctgattcgcg ccacgttcgg tgatatacgt cacttcatcg tcataatggg
    49801 cgcgggcgat aatctcaata tccgggtttt tcgcgcgggc agatgccaca atctcgcctg
    49861 cttcataacc gttgggaatc gtcaggatca gccattttgc acattccaga tgcgccagtt
    49921 gcataatttc ttcgttcgcc gcattgccca atactgcgcg gaccccgcgc tctcgcagct
    49981 catcaacacg ggttcgtgac gtctcaatca ccaccagcgg aatatcagag gcgagcaatt
    50041 tctcccccag caggctgcct acacgaccgt aacccaccag tagcgcatgg ttgcaaatat
    50101 ccactgggat ctgcttctct tcttcgattg cctcttccag cgtctgctct tccagcgttt
    50161 cggtcttcgc cagatatttc tccagtagtg cgaacagtac cgggttgagc ataatcgaca
    50221 ggatcgcccc tgccagtacc aggttttgtc cggcctgcgg cagtaaattc aatgccattc
    50281 ccagtcccgc caggataaac gcgaactcac caatctgcgc caggctggcg gcgatggtta
    50341 atgccgtacg ttgggagtga ccaaacagtc gcaccaggaa aaatgcggct aacgacttac
    50401 caaacagaat aatcgccagc gtcgccagca ctgccagcgg ttgctgaatc agaattaacg
    50461 gatcaaacaa catcccgacg gagacaaaaa acagcaccgc aaacgcgtcg cgcaatggca
    50521 gcgtatcgtg ggcggcacgg tgactcagtt cagactcgtt cagtaccatc ccggcaaaga
    50581 acgcaccgag tgcaaaggag acatcaaaca gctctaccgc accaaaggca atccctaacg
    50641 ccagcgccag caccgacagg gtaaacagct cgcgagaacc ggttgccgcg ctgcgtgcca
    50701 taatccacgg caccagacgg cgacctacca gcatcataat ggcgataaat gcgatcactt
    50761 tgccgatggt gatccccata tcgactgcaa gagtggcaaa gcccacatcg ccctgttcca
    50821 tcattcctgc cactgcgggc agcaacacca gcgtcagaac cattaccagg tcttccacaa
    50881 tcaaccaacc gatggcgatt tgcccacgct gactgtcaat taattgccgt tcttcaagtg
    50941 cgcgcagtaa caccacggta ctggcggtgg aaagacataa accgaacacg ataccggtca
    51001 ttaacgacca gcccagcacg gcagagagcg ccatacccag cagcgtcgcc acggctatct
    51061 gggcgatcgc accgggaatg gcgatggcct ttaccgccat caaatccttc agcgaaaaat
    51121 gcaaaccgac gccgaacatc agcaaaataa cgcccagttc agccagttcc ggcgcaagct
    51181 tggtatcggc aacaaagccc ggagtgaatg gtcctgccag cacacccgct aacagatatc
    51241 ccaccagagg agaaatacgt agtttattgg ccagcatgcc gaggataaag gcgagcacaa
    51301 ggccgccaac aatggtggtg ataagcgggg tggcgtgatg cattccgtct ccttttcctg
    51361 gtggttattg tccatttttg gccggggaaa ccaaaattac aggtaatagt ttatgacaat
    51421 ttcattgatg atgttcatga ataattattg aattttgcag aaaaatggaa ttagctgcaa
    51481 aaaaagcacg gatcggaaaa cagaaggcgg tttaacagag gaaaggctta tggcgactgt
    51541 aaaacgatgc agccaaagtt tgcctttggc tgcaatgaaa tcagtcttta tgccggttat
    51601 caggcaggaa tatggtcaac atccccaata gtggcaggaa agcacagatt ttatagacta
    51661 actcgatgct ggtgtgatcg gcgataagcc ccagaactgc cgctcccaga cctcccatgc
    51721 caaaagcaaa accgaaaaag agtccagaaa ccataccgat acgtcccgga agtagctcct
    51781 gagcgtagac cagaatggca gagaatgccg aagcgaggat aaatccaata atcaccgtta
    51841 aaacccccgt ccagtgcagg ctggcgtagg gtaaaatcag cgtaaacggc gcaacgccga
    51901 ggatagagcc ccaaatcaca tatttccgcc caattttatc ccctacaggc ccgccgatca
    51961 ccgtacctgc cgcaacggca aacaggaagg caaacagatg aagctgagca ttctggatag
    52021 ataatccgaa tttttgcatc agataaaagg tgtaatagct gctgatgctc gccatataga
    52081 aatatttcga gaaaatgagg attaacagaa tgctgaccgc cagtaccact ttgttgcgcg
    52141 gcagtggatt gataatcgtc gctttgggtt ttcctttatt cattcggtgc tgtgccgagt
    52201 accaacggct aatttgcgcc aacaccacga tcgccagcag tgccgcaagc acaaaccagg
    52261 caacgttgcc tttgccataa ggcgcgataa tcaccgccgc cagcaagggt cccagggaac
    52321 tgccaaagtt gccgccgacc tgaaagatag attgcgccag gccatgccgc ccgccggaag
    52381 ccatacgggc tacgcgagaa gattccggat gaaagaccga tgaaccggta ccgaccagcg
    52441 ccgccgccag cagaactgcg ccaaaactgc ccgccagcgc aagcagcacc agaccactta
    52501 aggtaaagca catgccaatt ggcaacgacc acggcatcgg atatttatcg gtccagtagc
    52561 cgaccactgg ttgcagtagc gaagaggcga gctggaaggt gagggttatc atgccaatct
    52621 gcataaatgt cagagaaaat tctgactgaa gcagcggata aatcgccaga atcagcgatt
    52681 ggatcatgtc gttcagcaga tgtgagaggc tgatagcacc taaaatacca aacgatgttc
    52741 gggccttggt cgttgacgca gccgcgcccg ccacaggctg gggttgttca ctcattgcca
    52801 taggaaagtc actttttcag ggttgcgatg taaagaatga tcttatttgt gattattacc
    52861 cgactaacat acctgtatgc gtcgtctgaa ggaagtctca acgccgaata cagaatttct
    52921 aatctggatg cagatttatc ttcaccggac gcagacttgt ctatgatgtc gcgtcatact
    52981 atttttcaac acgttgaaat caggtcaggg agagaagtat gaaattattg cagcggggcg
    53041 tggcgttagc gctgttaacc acatttacac tggcgagtga aactgctctg gcgtatgagc
    53101 aggataaaac ctacaaaatt acagttctgc ataccaatga tcatcatggg catttttggc
    53161 gcaatgaata tggcgaatat ggtctggcgg cgcaaaaaac gctggtggat ggtatccgca
    53221 aagaggttgc ggctgaaggc ggtagcgtgc tgctactttc cggtggcgac attaacactg
    53281 gcgtgcccga gtctgactta caggatgccg aacctgattt tcgcggtatg aatctggtgg
    53341 gctatgacgc gatggcgatc ggtaatcatg aatttgataa tccgctcacc gtattacgcc
    53401 agcaggaaaa gtgggccaag ttcccgttgc tttccgcgaa tatctaccag aaaagtactg
    53461 gcgagcgcct gtttaaaccg tgggcgctgt ttaagcgtca ggatctgaaa attgccgtta
    53521 ttgggctgac aaccgatgac acagcaaaaa ttggtaaccc ggaatacttc actgatatcg
    53581 aatttcgtaa gcccgccgat gaagcgaagc tggtgattca ggagctgcaa cagacagaaa
    53641 agccagacat tattatcgcg gcgacccata tggggcatta cgataatggt gagcacggct
    53701 ctaacgcacc gggcgatgtg gagatggcac gcgcgctgcc tgccggatcg ctggcgatga
    53761 tcgtcggtgg tcactcgcaa gatccggtct gcatggcggc agaaaacaaa aaacaggtcg
    53821 attacgtgcc gggtacgcca tgcaaaccag atcaacaaaa cggcatctgg attgtgcagg
    53881 cgcatgagtg gggcaaatac gtgggacggg ctgattttga gtttcgtaat ggcgaaatga
    53941 aaatggttaa ctaccagctg attccggtga acctgaagaa gaaagtgacc tgggaagacg
    54001 ggaaaagcga gcgcgtgctt tacactcctg aaatcgctga aaaccagcaa atgatctcgc
    54061 tgttatcacc gttccagaac aaaggcaaag cgcagctgga agtgaaaata ggcgaaacca
    54121 atggtcgtct ggaaggcgat cgtgacaaag tgcgttttgt acagaccaat atggggcggt
    54181 tgattctggc agcccaaatg gatcgcactg gtgccgactt tgcggtgatg agcggaggcg
    54241 gaattcgtga ttctatcgaa gcaggcgata tcagctataa aaacgtgctg aaagtgcagc
    54301 cattcggcaa tgtggtggtg tatgccgaca tgaccggtaa agaggtgatt gattacctga
    54361 ccgccgtcgc gcagatgaag ccagattcag gtgcctaccc gcaatttgcc aacgttagct
    54421 ttgtggcgaa agacggcaaa ctgaacgacc ttaaaatcaa aggcgaaccg gtcgatccgg
    54481 cgaaaactta ccgtatggcg acattaaact tcaatgccac cggcggtgat ggatatccgc
    54541 gccttgataa caaaccgggc tatgtgaata ccggctttat tgatgccgaa gtgctgaaag
    54601 cgtatatcca gaaaagctcg ccgctggatg tgagtgttta tgaaccgaaa ggtgaggtga
    54661 gctggcagta atccgaaagt gccggatgtt tgcatccggc acaatgctta atcgcggcgg
    54721 gcgatatcag caaatttggc atcgaggatc tttgccagat cgcctgccgc cagttcgata
    54781 tccagtccgc gcttgccgcc ggaaacataa atagtggcaa attcttgtgc gggggcgtcg
    54841 ataatcgttg gcagacgttt tttctgcccc agtgggctaa tccccccaac caggtatccc
    54901 gtcgaacgct gcgcgaccat cggatcggcc atctcaactt tcttggcacc cagcgctttt
    54961 gctacttttt taagatccag ttgacctgcg accggcgtaa cggccacggc aaggtgtttc
    55021 atatcaccgt tcactgccac cagcagcgtt ttgtagacct gatccggatt caaacctaat
    55081 tttttgacga cttcatcgcc aaaattggtt tcagccggat cgtgctcgta ggtatggatt
    55141 tgaaacgaaa tcttgttttt ttcgagtaat ttaactgcgg gtgtcatggt aattttcctg
    55201 ccttaaacaa aaaaacgaag caagcatacg cctgatggcg gtccaaaaaa aagagtcatc
    55261 ttgcctaaga gtattggcag gacggtgaga ttgagcgaca atcgagttac accgtcgctt
    55321 aaagtgacgg cataataata aaaaaatgaa attcctcttt gacgggccaa tagcgatatt
    55381 ggccattttt ttagcgcaac atttgcggca aattcccttc tccatacagg tgtagtgcac
    55441 cgaccgcgac cacatatcgc cccggcggca tggcgcgtaa tttatcccgc caggcgagat
    55501 ttcgctgatg catcagcaca tcgtacagcg actgactgaa cgtattgggc agcgttattt
    55561 cattattttg cggcggcgca ttcagccacc agctcatcat ttgttgcagc aaccgtgcgt
    55621 tggtatgcca gtgggtcagc gtatcgtcca gcagcgccag tcctttgtca gggagctgga
    55681 gcaacatggc aatctggttt tcagcccctt ccagttcaat cacgggttta tgttgttgct
    55741 tcgccgcctg caatagctgg taatcgatac cgtattctgc ccgcagcccc agtttttgtg
    55801 cctgcgtcgc ctgaagaacc atcgcgattt gccacagcgg ttgggtagaa aagagtgagg
    55861 gagaaatgcc catctcctgg ctaatgtgct gcagattttg gagttgctcc tcgctaatgc
    55921 gctcttccag cgcctcgcag gcaggcaaat tagcaaaagg cgtatcgctg gtggaaacat
    55981 ccgcctcgac gatcagcgca tcggcgtttt tgagcttttt gagcaaacgg gtgggcaggg
    56041 gagccatatc gtggctaccc atatgaatac tgccaatcag atgaaaatgg cgattgccgg
    56101 ggagggtgat atcgatggca ggccaggtgt aatgattacc gcgcagcgcg gcccaaagcg
    56161 tttttacccg gtacaacaga tccatacgac ctccctttgt gaaatatcat gctagcgcgc
    56221 ggtgagggat ggcgcaaatg gagttgggga cttgtttaat gtgtttgtat gattcagtat
    56281 gttcttgcat cgctattcac agggaagcaa cagttaaaaa ccatgaaaca ggcaacaaga
    56341 aaaccgacga cacccggaga tattcttctc tatgaatatc tggaaccgct cgatttgaaa
    56401 atcaatgagt tagcagagtt gctgcatgtt catcgtaata gcgtcagtgc actgatcaat
    56461 aacaatcgta aactcaccac tgagatggca ttccgtctgg cgaaagtttt tgataccaca
    56521 gtcgatttta ggctaaacct ccaggcggca gttgatcttt gggaagttga aaacaacatg
    56581 cgcacccagg aagaattggg acggattgaa acagtggctg aatatttggc acgccgtgaa
    56641 gagcgtgcaa aaaaggtcgc gtaaggccaa aacatcgctt catctcgtag gccggataag
    56701 gcgtttacgc cgcatccggc atccgagcac cattgcctga tgcgacgctt acccgtctta
    56761 tcaggcctac aaacctgtgc ctgaaccgta ggccggataa ggcgtttacg ccgcatccgg
    56821 cagtcatgcg tcgatgccaa atgcgccgcc ctaaagcagc gcatccgcaa tgacgtactt
    56881 attccttcgg tttaaaccgc agcaaccggt tggcgttact cactacggta atcgacgaga
    56941 gcgccattgc cgctccggca actaccgggt taagcagtgt tccagtaaac ggccacaaaa
    57001 taccggcggc gaccggaata ccgatgctgt tgtagataaa ggcaccgagc aggttctgct
    57061 tcatgttgcg cagcgttgcg cgggaaatcg cgagcgcatc cgcaacgccc atcaggctat
    57121 ggcgcatcag ggtaatcgcc gcggtttcaa tggcaacatc actgccgcca cccatcgcaa
    57181 tgccgacatc cgcctgggcc agcgctggcg cgtcgttaat gccgtcgccc accattgcca
    57241 cctgacgtcc ttcactttgc agacgtttga tcgcttcggc tttaccgtcc ggcagcactc
    57301 cggcaatcac ttcatcaatt ccagcttctt tggcgatcgc attggcggtg gttgggttat
    57361 ccccggtcaa catcaccaga cgatatcccg ctttatgcag gcgttgcagc gccgccacgc
    57421 tatcactacg caacggatcg cgtacagcca gcagggctac tgctttcccg tcaatcgcca
    57481 gcagcacagg cgttgctcct tgcgatgcct gagcagtaat ctccgcttcg atagctttgg
    57541 taccaacctg ttgctcattt aacagcgcct gattgcccag caataacgca tgaccttcag
    57601 cttcaccgct cacgcccagc ccgcgcaatg tgcggaaacc gttgacctgc ggcagctgca
    57661 tatcacctgc tttatcgagg atcgcccgtg ccagcggatg gctggaacct tgctccagtg
    57721 ccgccgccag acgcaatgcc tgcgcttcat caacatcagc aaacgttttc actgcgacaa
    57781 cctgcggctt cccttcggtc agcgtcccgg ttttatcgaa cacgacggta tcgagtgtac
    57841 tggcgcgttg cagcgcgtcg gcgtcccgca ccagcacgcc aaactcagcc gcccgcccga
    57901 cgccggaaat aatcgacatc ggcgtcgcca gccccagcgc acacggacag gcgataatca
    57961 gtaccgtggt ggcaatcacc agggtataaa caatttgcgg tgccggacca aagaagtacc
    58021 agattgccgc actgaccagc gcaatcacca ccaccacagg tacaaatacg gctgagattt
    58081 tatccgccag ctgaccgatt tctggcttgc tgctctgggc ctggcgcacc atgcgaatga
    58141 tccgtgacag cgtggtatgg ctgccaaccg cactggcacg aaacagcaca ctgccgtcct
    58201 gtaccactgt cccggcatgg acgctttcgc cttcgccttt ttgctgtggg attggttcgc
    58261 ccgtcagcat cgcttcatcc agccatgctt cgccctgggt aatctcgcca tctaccggca
    58321 cgcgatcgcc ggtcgtcagg cgcagcaaca tacctggctg cacttccgcc agaggcacgc
    58381 ttttttcacc ttcgtcagta accaggcgcg ccgtcggtgg ggttaaatcg agtaatttct
    58441 ccagcgcctt agaagaacgc tgacgtgcgc gcgcttccag catatggccg agattaatca
    58501 gaccgataat catcgcactg gcttcatagt aaagatgccg cgcttccatc gggaaccact
    58561 gcggccacag gttgacgctc atcgaataga gccacgccac acccgttccc agcgccacca
    58621 gcgtatccat cgtcgctgca ccgttcagca ggcttttcca tgcactgcgg taaaaatggc
    58681 cgccggcgaa aaccatcacc gccagggtta tcaggccgat aaccaaccac aggctgcggt
    58741 tgtcagcggt gaccatcatg ttatcgccaa tcatccccca gaccatcacc gggataccca
    58801 ccgccagtgc gacaattgcc tgccagcgga agcgcttcat cgtagcaacg gcggtttcct
    58861 gctggcgctc acggcgtttc gcgtcatctt caatcgcttc cgcgccgtag cccgcttttt
    58921 ccaccgcctg cactaaatct tgtggggagg cactgcccat caccagcgca gtacgctccg
    58981 ccaggtttac ccgtgcctga gtgacgcccg gtacgctttg cagcgcattt tgtacgcggg
    59041 tgacacagct ggcgcagctc atgccgctca gcagcaactg ctggctgtca tcgtcatcgg
    59101 cggtcgctgc cggaagcgcc tcagaaaccg ctgtcagtgc ttccgacggg attgatgact
    59161 ccgccagcgg tttagccttt gggtggctta cagatgcgtc ataacccgct tgtttgatgg
    59221 tttcgatcag ctgttctgca ctggcagtcc cggtaacgtg cgcttcagtg atagacacat
    59281 ccgcttgttc cacatccggg cgctgttcga gactttcttt cacgcgttta acgcagtgac
    59341 cgcaggacag gccgtccagg gtcaggtcga taatttgtga cataaaacac tcctttaaga
    59401 cagttttgac tggctgtgat aaaggttaaa ccttccagca aggggaaggt caagaaatta
    59461 ataaaccagg cgagtaaaag tccgtaaaga ttaaaaaatt ggctcaattt gcatcaggat
    59521 tagacattta tctctttgtt ttcctgtagt taagttgcgg gtgctaagtt aaatcaggat
    59581 gcctcaaaat cggcaccggg gtgaggaatt acctcccgca tctataaaaa ggagttaaca
    59641 aaagatgtta gatgcaaaca aattacagca ggcagtggat caggcttaca cccaatttca
    59701 ctcacttaac ggcggtcaaa atgccgacta cattcccttt ctggcgaatg tgcctggtca
    59761 actggcggca gtggctatcg tgacctgcga tggcaaggtc tatagtgcgg gtgacagtga
    59821 ttaccgcttt gcgctggaat ccatctcgaa agtctgtacg ttagcccttg cgttagagga
    59881 tgtcggcccg caggcggtac aggacaaaat tggcgctgac ccgaccggat tgccctttaa
    59941 ctcagttatc gccttagagt tgcatggcgg caaaccgctt tcgccactgg taaatgctgg
    60001 cgccattgcc accaccagcc tgattaacgc tgaaaatgtt gaacaacgct ggcagcgaat
    60061 tttacatatc caacagcaac tggctggtga gcaggtagcg ctctctgacg aagtcaacca
    60121 gtcggaacaa acaaccaact tccataaccg ggccattgcc tggctgctgt actccgccgg
    60181 atatctctat tgtgatgcaa tggaagcctg tgacgtgtac acccgtcagt gctccacgct
    60241 cctcaatact gttgaactgg caacacttgg cgcgacgctg gcggcggggg gagtgaatcc
    60301 gctgacgcat aaacgcgttc ttcaggccga taacgtgccg tacattctgg ccgaaatgat
    60361 gatggaaggg ctgtatggtc gctccggtga ctgggcatat cgcgttggtt taccgggcaa
    60421 aagcggtgta ggtggcggta ttctggcggt cgtccctggt gtgatgggaa ttgccgcgtt
    60481 ctcaccaccg ctggacgaag aaggcaacag tgttcgcggt caaaaaatgg tggcatcggt
    60541 cgctaagcaa ctcggctata acgtgtttaa gggctgatca tgatgaacac ggaaggtaat
    60601 aacggtaaca aacccctcgg tctatggaac gtcgtttcca tcggcattgg ggcaatggtg
    60661 ggggcgggga tcttcgcgct gctggggcag gctgcgttgc tgatggaagc ctcgacctgg
    60721 gtcgcctttg cttttggcgg tattgtggcg atgttttccg gttatgccta tgcgcgtctg
    60781 ggggcgagct atcccagcaa tggcggcatt atcgacttct ttcgtcgcgg attaggcaac
    60841 ggcgtctttt cgctggcgct ctcgttactg tacctgttga cgctggcggt gagcatcgcc
    60901 atggtcgccc gtgcttttgg cgcttatgcc gtgcagtttt tgcatgaagg cagccaggag
    60961 gagcacctta ttttgctcta cgcgttgggg atcattgcgg tgatgacgct tttcaactcc
    61021 ttaagcaacc atgcggtagg gcggctggaa gtgatcctcg tcggcattaa aatgatgatc
    61081 ctgttattgc tgattattgc cggtgtctgg tcgctgcaac cggcgcatat ttccgtctct
    61141 gcgcccccca gctccggtgc gttcttctcc tgtatcggga taaccttcct tgcctatgcg
    61201 ggctttggca tgatggcgaa cgcggcggat aaagtgaaag atccgcaggt cattatgccg
    61261 cgggcgtttc tggtggcgat tggcgttacc acgttgcttt atatctcgct ggcactggtt
    61321 ttgcttagcg atgtatcggc attagagtta gaaaaatatg ccgataccgc cgtagcgcag
    61381 gctgcttctc cgctgctcgg acatgtgggt tatgtgatcg tcgtcatcgg cgctttactg
    61441 gcgacggctt cagccattaa cgcgaacctg ttcgccgtgt ttaacatcat ggacaacatg
    61501 ggcagcgaac gcgaactgcc gaagctaatg aataaatccc tgtggcggca gagtacctgg
    61561 ggcaacatca ttgtcgtggt gttgattatg ctgatgacgg cggcgctaaa tttaggctca
    61621 ctcgccagtg ttgccagcgc cacctttttg atttgctacc tggcggtgtt tgtggtggcg
    61681 atccgcctgc gtcatgatat tcacgcctcg ttgccgattc ttatcgttgg tacgttggtg
    61741 atgttgttgg tgatcgttgg ctttatctac agtctgtggt cccaggggag ccgtgcgttg
    61801 atatggatta ttggctcact cttactcagc cttattgtgg caatggtcat gaagcgcaat
    61861 aaaaccgtat aacatctctc tgtgcgcagt acttcctgta ttattgtggt ggcggtcgat
    61921 attcgcactg gcaaaaaaac gtgcttgaat atctgttgaa accctttaac aaagcacagg
    61981 aggcgttgcg cgaacgatga acatcagcga tgtagcaaaa attaccggcc tgactagcaa
    62041 agccattcgc ttctatgaag agaaggggct ggtgacgccg ccgatgcgca gcgaaaacgg
    62101 ttatcgcacc tacacgcagc agcatctcaa cgaactgacc ttactgcgcc aggcacggca
    62161 ggtgggcttt aacctggaag agagcggcga gctggtgaat ctgtttaacg acccgcagcg
    62221 gcacagcgcc gacgtcaaac ggcgcacgct ggagaaggtg gcggagatcg aacgacacat
    62281 tgaggagctg caatccatgc gcgaccaact gctggcactg gcgaatgcct gccctggcga
    62341 tgacagcgcc gactgcccga ttatcgaaaa tctctccggc tgctgtcatc atcgggcagg
    62401 gtgattaaga cgagacggct cggatatgta gggttatccc ttctatcgca atgacttcaa
    62461 catgcgtacc tgcaccgaga tcctcgctgg cgctgacagg ccatgaactg tcaccgacgc
    62521 gcatatgacc gcgcccgttg accagcggag attccagcac aaaacgtcgg ccaatcagct
    62581 gctgcccgcg ctggtttaaa tgactatcgc tgtgcttttg ttcgcgcacc cgccgcgaca
    62641 accatttcca ccacagccag gcggcgagca gcgtcaggat ggcaaacatt accccttgcc
    62701 actcccaacc cagcggcacc agccagacca ccaggccggt aatcaccgct gccacgccgc
    62761 tccacaacaa ataaccattt ccgcccagca tctcggctgc cagcagcaaa ccgccgagac
    62821 tgagccagaa aatatgtgga tgaacgacca ttaactccat catggctgag tccgtttgtt
    62881 ggcgctgtct ttcaccagct cggcaatccc ggcaatcgac cccatcaggc tgctggcctc
    62941 taatggcatc atcaccactt tgctgttact ggacgaaccg atctgctgta acgcttcggt
    63001 gtatttctgc gctacgaagt agttcaccgc ctgaatatca cctgaggcga tggcttcaga
    63061 caccattttg gtggcgcggg cttctgcttc agcggaacgt tcacgcgctt cagcctgtaa
    63121 aaacgccgac tgacgttcgc cttccgcttt caggatttgc gactgttttt caccttcggc
    63181 tttgaggatt tccgcctgac ggatcccttc cgcttcaagg atgtaagcgc gtttggtacg
    63241 ttccgctttc atctgcgcgt tcattgaaga gataagctct gccggtgggc gcacgtcgcg
    63301 aatttcaata cgggtgactt taatccccca cgggttggtg gcttcatcga caatacgcag
    63361 caggcgtgag ttgatgctgt cgcgctgaga gagcatttca tcaagttcca ttgaacccag
    63421 cacggtacgg atgttagtca tggtcaggtt gatgatagcc agctccagat tgctgacctc
    63481 ataagccgcg cgtggcgcgt caatcacctg aataaagcac acggcgtcga tggtaacgtt
    63541 ggcgttatct ttcgagataa cttcctggga agggatatcg agcacttgct ccatcatatt
    63601 gatcttgcga ccaatgcgat ccataaacgg caccaccaga ctgagccccg gctgtaacgt
    63661 tttggtatag cgaccaaaac gttctaccgt ccattgatag ccctgcggca cgattttgac
    63721 gcccgcgccg acaatgacca gcgcgacaaa aatgagaatc gggataaaga taagcatcgg
    63781 aaaaacctcc tgttgtaccg tccataatca gcaaaattgc tgcttgatta aacaaattat
    63841 acctgattac tgaaagagag ttccccctta ttcctgcgaa ggataaactg tttttagtaa
    63901 aaatcagaaa aagggaacag cgatgcagga aaatagtcct ttgcttcagc tacaaaacgt
    63961 aggatatctg gcgggtgata cgaagattct taataacatc aatttttcgc tgcgtgctgg
    64021 cgaatttaag ttaattaccg gtccttctgg ttgtggcaaa agtacgctgc taaaaatagt
    64081 tgcttcattg atcagcccaa ccagcggaac gttactgttt gaaggggagg atgtcagcac
    64141 actaaagcca gaaatctacc gccagcaagt ctcttactgc gcccagacac cgacgctgtt
    64201 tggcgatacg gtatacgata atctgatctt tccctggcag atccgtaacc agcagcctga
    64261 cccggccatt tttctcgatt ttctcgaacg cttcgctttg ccggacagca ttttgacgaa
    64321 ggatatcgcc gagctatctg gtggtgaaaa acaacgcatc tcattgattc gtaacctgca
    64381 atttatgccg aaggttttat tgctggatga aataaccagt gcgctggatg aaagtaataa
    64441 acataacgtc aatgagatga tccatcgtta tgtgcgcgag caaaatattg ccgtgctgtg
    64501 ggtgacacac gataaagacg aaattaatca tgcggataaa gtgattacac tgcaaccgca
    64561 tgccggagaa atgcaggaag cacgctatga actcgcataa tattactaac gaatcattag
    64621 cactggcatt aatgctggtg gtggtggcaa tcttaattag ccataaagaa aaactggcgc
    64681 tggagaaaga tattctctgg agcgtcgggc gagcgataat tcagctgatt attgtcggct
    64741 atgtgctgaa gtatattttc agcgtggatg atgccagcct gacattattg atggtgttat
    64801 ttatctgctt taatgcggcg tggaacgcgc aaaaacgcag taaatatatt gctaaagctt
    64861 ttatctcatc gtttgttgcc attacggtcg gggcgggaat taccctggcg gtgctgattc
    64921 tctcagggtc gattgaattt atcccgatgc aggtgatccc tatcgccggg atgattgccg
    64981 gtaacgccat ggtagcggtg gggttgtgtt acaacaattt agggcaacgg gtcattagcg
    65041 aacagcaaca gattcaggag aaactgagtc ttggtgcgac gccgaagcag gcttcagcga
    65101 tattgattcg cgacagtatt cgcgcggctt taattccgac ggtcgattca gcaaaaacgg
    65161 ttggcttagt gagtttacca ggaatgatgt ccgggctgat atttgccggg attgatccgg
    65221 tgaaggcgat taaatatcag attatggtga cctttatgct gctctcaacc gccagcttgt
    65281 cgaccattat tgcctgctat ttaacctatc gtaagtttta taattcgcgc caccagttgg
    65341 tggtgacgca attgaagaag aaataatgcg atgccggatg cgcagcatcg catccggcgt
    65401 tgtggttcat gtgccgatca acggaatatc aatacaacaa tgcatacagc tggcggcgat
    65461 acttcgacgc cagtgcatca cccgtaccca gcgcagcgag gatctcctgg aacgttttac
    65521 gcgtctgacc gtctgcggcg gtgagatctt tacgcagatg cccgaacagc aactccagcg
    65581 cctcttcatt gcgcccaacc tggtgcagtt gcagagccag ttgcgtcgcc agtgcggcat
    65641 cttctggatt ctccgccacc tgctgttgca actgttgaat ttccggcgta tcagccgcct
    65701 gcttcagcag ttcgatttgc gccaccagcc cctggtagcg ggtgtcctga tcctgcaacg
    65761 gaatggtttt cagcaccgct tccgcatctt cagaacggtt cagcgcaatc agcgtttctg
    65821 ccagcagcag gccgatctcc ccgttctgat tcgacaactg ccaggcgtct ttcagcaatg
    65881 gcagggcatc ggtgtaattg ccttcctgca tcagttgcat cgcctgctgc gctttcagct
    65941 cttcttcgcg cggcagcact ttatccagca gggcgcggat cgcctcttcc ggttgcggcc
    66001 cctggaagcc atctaccggt tgcccgttct ggaacagata cacggtcgga atcgcgcgca
    66061 gaccaaactg ggcggcaatc atctgctccg cgtcgcagtc cagcttcgcc agaataaatt
    66121 gcccgttgta ctgcgccgcg aggctttcca ggatcggggt taactgcaaa cagtgctggc
    66181 tacgttcaga ccaaaaatag aacagcaccg gagtggtcat cgactgttca agaacctgtt
    66241 gcaggttaga ttcgttaatg ttgacaatat tttctacgga catggagtcg ctctctgttg
    66301 tcgatatttt ctttgacatg ggggcttaag cgcgcgcttc aactcacccc tgcaatattt
    66361 tgtccatcac gcgccccggc agcaggcgct taagcaccat tactgcccag gtcaccagcg
    66421 ttaccggata gcgcatcttc ggcttctcgc taataaaagc atggcgtact ttgtccacca
    66481 ccgcttccgg tcccaacgta aagcgggcgg cgatgccggg attttcgact ggtttatcac
    66541 tttgcgtctg gttgacgttg tcggtgaagc gagtacgaat gggaccgggt tcgatcaggc
    66601 tgactttaat tccgctgtgg cgtagctcca tgcgcagcgc gtctgaccac gcctccagcg
    66661 catatttact ggccgcgtaa gcgccacgac ccggcgtgga gattaatccc atcaccgatg
    66721 atgtcatcac aatacgccct tcaccgtgcg gtaacatcgc gggtaacagg cgcatggtga
    66781 gctggtgtgc gccgaaaaag ttggcggaaa actgctgttc catctgcgca cggctgatgg
    66841 tggaaagggg gccatacatg ccgaatccgg cattgttaaa gatcccatac agacaattat
    66901 cggtcagggc gatcacttcg tcggctgcgc gatcaacact ttctggcgaa tccagatcga
    66961 tcaacacgcc ggtaaatccc atgctgttca tacgctcaac atcatccggt ttccggcaac
    67021 ctgccagcac atgaaaaccc tggcgtttta attcgagcgc gctttccagg ccaattccac
    67081 tggaacatcc ggtaattaag accgattttt gcataacttt acctgtcagg atctccgttg
    67141 ctttatgagt catgatttac taaaggctgc aactgcttcg ccatccagtc ggcaataaac
    67201 ggctgggcgt cgcggttggg atgaataccg tcatcctgca tccattgtgg cttgaggtag
    67261 acctcttcca taaaaaaggg cagcagcgga acatcaaact ctttggcgag tttggggtaa
    67321 atggcgctaa aggcttcatt ataacggcga ccatagtttg caggcagacg tatttgcatt
    67381 aacaatggtt cagcgttggc ggctttgaca tcctgcaaaa tctggcgcag cgtttgctcg
    67441 gtttgctgtg gctgaaaacc acgcaaaccg tcattgccgc ccagttcaac cagcacccaa
    67501 cgcggctgat gctgtttcag cagagccgga aggcgcgcca gtccttgttg cgaggtgtcg
    67561 ccgctgatgc tggcattaac taccgacgtt ttactctgcc acttatcatt caacaaggca
    67621 ggccaggccg cgctggcaga cattcgatac ccggcgctca ggctatcacc cagaatcaat
    67681 aacgtgtccg ctgcggcggc acggaaggtt aacaggacca ggaacaggaa gggcaaatgc
    67741 cagcggaaaa cattgttgaa gttcatcatc ttaagaagtc cgtcggtcag ggggagcatg
    67801 aactctccat cctcaccgga gttgagctgg ttgtcaaacg tggcgagacc atcgcactgg
    67861 tgggcgagtc gggatcgggt aagtcaacct tgctggcgat cctcgccggg cttgatgacg
    67921 gcagcagtgg cgaagtgagt ctggtgggac aaccgctaca taatatggac gaagaagcgc
    67981 gggcaaagtt gcgcgcgaag cacgtcggct ttgtttttca gtcatttatg ttaattccta
    68041 cccttaacgc gctggaaaac gtcgagcttc cggctctgct gcgcggtgag agtagcgcgg
    68101 aaagtcgtaa cggggcgaaa gcgttgctcg aacagttagg gctgggtaaa cgtctggatc
    68161 atcttccggc acagctttcc ggcggtgaac agcaacgagt ggcgctggca cgagccttta
    68221 atggtcgacc tgatgtgctg tttgccgacg aacccaccgg caaccttgac cgccagacgg
    68281 gcgataaaat tgccgacctg ctgttttccc tcaaccgtga acatggcacc acgttgatta
    68341 tggtgaccca cgacctgcaa ctggcggcac gctgcgaccg ctgcttacgg ctggtgaacg
    68401 ggcagttgca ggaggaagca tgattgcacg ttggttctgg cgcgaatggc gttcgccgtc
    68461 gctattaatt gtctggctgg cgctaagcct ggcggtggcc tgcgtgctgg cgctgggcaa
    68521 tatcagcgat cgcatggaga agggcttaag ccagcaaagc cgtgagttta tggcgggcga
    68581 tcgggcgttg cgcagttcac gcgaagtgcc gcaagcgtgg ctggaggaag cgcaaaagcg
    68641 cggcctgaaa gtcggcaagc agctgacttt cgccacaatg acctttgcag gcgacacacc
    68701 gcagctggcg aacgtcaaag cggtggatga tatctacccg atgtatggcg atctgcaaac
    68761 taatccccct ggcctgaaac cgcaggcggg cagcgtattg ctggccccac gcctgatggc
    68821 actgcttaac ctgaaaacgg gcgacaccat tgacgtgggc gatgccacct tgcggattgc
    68881 cggagaagtg attcaggaac cggattccgg ttttaacccc ttccagatgg ctccgcgtct
    68941 gatgatgaat ctggcggatg tcgataaaac cggagccgtg cagccgggga gtcgggtcac
    69001 ctggcgttat aaattcggcg gcaacgagaa ccagctcgac ggctatgaga aatggttgtt
    69061 acctcagctt aaacccgaac aacgctggta cggtctggaa caggacgaag gcgcgctggg
    69121 gcgatcgatg gaacgctcgc aacagttcct gctgctttcg gcgcttctga ccttgctgct
    69181 ggcagtggca gcggtggcgg tagcgatgaa tcattactgt cgcagtcgct acgatctggt
    69241 ggcgatcctc aaaacgctgg gggcagggcg agcgcaactg cgtaagctaa tcgtcggtca
    69301 gtggttgatg gtgctgacgc tttcagccgt taccggtggg gccataggtc tgttgttcga
    69361 aaacgtgttg atggtgctgc tcaagcccgt tctgcctgct gcactaccgc cagccagcct
    69421 ctggccgtgg ctgtgggcgc ttggcaccat gacggtcatc tcgctgctgg tggggctacg
    69481 accataccgc ttgttgctgg caacgcagcc tttacgcgta ttacgtaatg atgtggtagc
    69541 gaacgtctgg ccgctgaagt tttatctgcc gattgtcagt gtggtggttg tgctgctgct
    69601 cgccggatta atgggtggca gcatgctgct ttgggcggtg ctggcgggcg cggtagtact
    69661 ggctttgctg tgcggtgtgc tgggctggat gctgctgaat gtacttcgcc gcatgacgct
    69721 gaaatcgctg cctctgcgcc tggcggttag ccgcctgtta cgtcagccgt ggtcaacgtt
    69781 aagtcagctt tcggcatttt cgctctcctt tatgctgctg gcgctgttgc tggtattgcg
    69841 tggcgatctg ctcgaccgct ggcagcagca gctaccgcca gaaagcccga actacttttt
    69901 aattaacatc gccacagaac aggtagcacc gctaaaagcg ttcctcgcgg aacatcatat
    69961 agtcccggaa tcattttatc cggtagtgcg ggcgcggctg acggcgatta acgataagcc
    70021 gacagaaggt aatgaagatg aggcgcttaa tcgcgaactc aatcttacct ggcaaaatac
    70081 gcggcccgat cataatccga ttgtcgccgg taactggccg ccaaaagccg atgaagtgtc
    70141 gatggaagag gggctggcga aacgcttaaa cgttgccctc ggtgataccg tgacttttat
    70201 gggcgatacc caggagttcc gcgctaaagt gaccagcctg cgcaaagtgg actgggaaag
    70261 tctgcggcct aatttctatt ttattttccc ggaaggggca ttagacgggc aaccgcagag
    70321 ctggcttacc agtttccgct gggagaatgg caacggcatg ttgacgcaac tcaaccgcca
    70381 gttcccgacc attagcctgt tagatattgg cgcgatttta aaacaggtcg gtcaggtgct
    70441 ggagcaggta agtcgggcgc tggaggtgat ggtggtgctg gtcaccgcct gcggtatgtt
    70501 gctgttgctg gcacaggtgc aggtgggaat gcgccagcgt catcaggagc tggtggtatg
    70561 gcgcacactc ggtgcgggga aaaaactgct gcgcacgaca ttgtggtgtg agttcgccat
    70621 gcttgggttt ttttccggcc tggtggccgc aattggtgcg gaaacggcac tggcagtgtt
    70681 gcaggcgaaa gtgtttgatt ttccgtggga gccagactgg cgattgtgga ttgttctgcc
    70741 gtgcagcggt gcgctgctgc tgtcgctttt cggcggctgg ctgggtgcgc gactggttaa
    70801 gggtaaggcg ctgttcaggc agtttgcggg gtgatgacag gcgttagatt gcctgatgcg
    70861 ctacgcttat caggcctacg aagagcgtgc aatatattga tttttcaaga ttgtgtaggc
    70921 cggataaggc gtttacgccg catctggcat gaacagtgtg cattttggca ataaatatga
    70981 aaccaactgt catgtcggtt tttttaaagg ccgaaggtgg taaaccttcg gccttagcta
    71041 cgtttcctca gttattcaat aaactgacgg atcagttcca gcagcgcgat tagcgcctta
    71101 agaacgatga gaactttatc gagaaaagtt ttcatccgtt gctcccttaa gggagaaaga
    71161 caatcacagc ttacccttac gttgcctgta actatgattt tcttcatgtg ctagatgagg
    71221 actgcctctc tccaaattag agcaccagaa tcagcaccac ctgtatcctg accaggactt
    71281 aaaagtactt ttccgcactg cggtaatccc ttgaggcagt atcactacta gcgttgggat
    71341 tgtatcgcca acatagctgt ttgaaatgta ggcatcctga cttataattc acttgctaga
    71401 tgaggactgt ttcatttctg cccggaacca ccaagtcttg ggcggaaaaa ataaaaaaac
    71461 cgactgtcat gtcggttttt ttatatctgt attctggtgc aaccattctg ttctcacgac
    71521 aatcgccgtt tcagaatacc gcccatcact gcgccttaac ccattccgcc acttccgccc
    71581 actcaccgcg aaagacaact ttttccgctt ttttctcaag ctgatagcga tacatcgggt
    71641 cgtaatattc ttcaagtaac ggcaccagcc aggccagatg accgtcggtg ctgccggtgg
    71701 tgagttgcgt tgtcagtgct gcatccagcc ttgcagccag ttcgttatat cgctgtagcc
    71761 ccagccgacg cttaatcgcc gaaagtccgt gatgcaggta ttcgcaatat tcctgccagc
    71821 cctgttcgtc gccataagcg tgggtaaaat catgatgcat acgcaagaaa tactcttcgt
    71881 tcaggcgttc aagacggatc tcaaacggat cttctaccac cgcaatcgcc gcctgagtca
    71941 ttcgctcgcg cagacattcc ggcaggtgat tcgaaccgat catccggctt tcgtcttcca
    72001 gcacccacag gcgcaaattc tgacgggcgt cggtttttag catttcggca gccagcaggt
    72061 tttcaaaact cgcctggcta agttgtggtt gtaaggtgcg accaaacgcc gaaccgcgat
    72121 gacgcgccaa cccttccaga tcaacaccgt tcggctgttg ctgcactaac agcgttttgc
    72181 cgcagccggt acaaccgcca atcagcacta tcggtttttg tgacagttca atggtcgcct
    72241 gaatcgcagt ctggcgcagt accttataac cgccttccac cagcggataa ttaatcccca
    72301 cttcatgcaa ccagcgttgc acaatatgtg aacgttgacc gccacgggcg cagcagagaa
    72361 taccttgcgg attttgcagg cacgctgccc gccaggcgtc catgcgctgc tgacgaattt
    72421 cacccgccac cagtttatgt cccagcgcca gcgctgcgtc tgagccttgc tgtttatagc
    72481 aggtgccaac ggcggcgcgt tcatcgttat tcattaacgg cagattgata gcggcgggca
    72541 ttgcgccgtg ctcaaactcg ataggggcgc gaacatcaat aatgggcgta tcagcaatca
    72601 gcagggcacg atagtcctgt tccgtgtgtc tctcttgcat agttaaaagt gaacctcaaa
    72661 tcagcttgcg cgctatttta cgcgccaacg cgcaaggaaa cttgattttt aactgcgtgg
    72721 gttgccgaaa atttctaaaa atccgctgat ttccggcctg cgttgggtaa acaaggtcac
    72781 aatatcttct accgctttgc cgctgccaaa tttgcgccat gccagactca atggcgaagg
    72841 agggcgcatc gttgggatta cccggctgac cagttgttga ttatcgatca ttgactggca
    72901 aagtgatttt ggcaaaaaac caatgccaac gcccgccaga tgggcggcga ttttcgtttc
    72961 catatcagga acaataatct ctttttgccc tggcaatcgc caggcgacgc gtttggttaa
    73021 ggtgcgggcg ctgtcttcaa tattgaccgc cggaaagcgc cgcaactgcg cttctgttag
    73081 cggctcttca acgttcgcca gcggatgatc cgccgccatg acaaagcgcc attgcaccga
    73141 tcctaaggga tcaagactaa aggtatttgc cagcgcctcg gttcccgtga cgccgatagc
    73201 cagtgaaaaa ccttcgtaca atagcgagtc ccagacgccc atatagattt gtcgggagat
    73261 gtgaaactgg gtaaaggggt aacgttcatt cagccacgcc agcaactggg cgacggcctg
    73321 ggggttgtag agcaggttgt tgatgacaat attcacctgg cgttccacgc catcattcac
    73381 ctgttgcagc tcgcttggca tactttccag ccagctcagc cagtctctgg cctgggaaag
    73441 tagatgctcg ccagccgctg tcaacgtcac gctgcgagtc gtgcggaaaa acagcgctac
    73501 tccggtattc tcttccagaa gtttaatgcg ataactgatc gtcgctgtgg ttttacataa
    73561 tcgttctgcc gcttttgaaa aacttcctgt ttcagcaacc gcaatgaaag tccgcaaggt
    73621 ttctggatcg aacatcttca ggtatcccct tttaaatccg caagttgcgt gattttctta
    73681 tcctctgatt tatcagtatt tttacatgat aaccctgttc aatttgtgga ctaaatctag
    73741 ttttggaaaa atattccaac ttttgtattg aagttgttct cttaaggttt tagattgcct
    73801 gttattgaaa ccaagctgac cggtcggcgg tggttgaacg gaattatgtt acaaggacaa
    73861 aaagatgaaa cttcaggtat taccgttaag tcaggaagcc tttagtgctt atggcgacgt
    73921 aatcgaaacg cagcaacggg attttttcca tattaacaat ggcctggtgg agcgttacca
    73981 cgatttggcg ctggttgaga ttcttgagca agaccgtacg cttatcagca ttaaccgcgc
    74041 gcaaccggcg aatctaccgc tgaccattca cgaactcgaa cgtcatccgc tgggtactca
    74101 ggcctttatc ccgatgaaag gtgaagtgtt tgtggtggtc gtggcgttag gtgacgacaa
    74161 accagacctg tcaaccctgc gggcgtttat caccaacggt gaacaggggg tgaattacca
    74221 tcgtaacgtc tggcatcacc cacttttcgc ctggcagcgc gtcaccgatt ttctgaccat
    74281 cgatcgcggc ggcagtgaca actgtgatgt tgaaagtatt cctgaacagg aactctgttt
    74341 tgcgtgacgc ctgcaaccga cttgcataag ataaattaat tgttcattgt ttatgctcac
    74401 ttgtaggtcg gagttaacgt aggtatgacg gaagttagac ggcgcggcag gccaggacag
    74461 gcggagcctg tggcacagaa gggcgcacag gcgttagagc ggggaattgt gattctgcaa
    74521 tatctggaaa aaagtggggg aagttcgtcg gttagcgata tttctctcaa tctggatttg
    74581 ccgctctcca cgacctttcg cttgctgaag gttttacagg cagcggattt tgtctatcag
    74641 gacagtcagt taggctggtg gcatatagga ttaggtgtct ttaacgtcgg tgcggcgtac
    74701 atccataacc gcgatgtcct ctccgtcgcc gggccgttta tgcgccgcct gatgttactt
    74761 tccggcgaaa cggtcaatgt cgcgatccgt aacggcaatg aagcagtatt aattggtcag
    74821 ctagagtgta aatcgatggt caggatgtgt gcgccactgg gcagtcgtct gccactgcat
    74881 gcttccggtg cgggcaaagc gctgctttat ccgctggcgg aagaggagtt gatgagcatc
    74941 attctgcaaa ccggtttgca gcagtttacg ccaactacgc ttgtggatat gcccacctta
    75001 ctgaaggacc tggagcaagc gcgtgaactg ggctataccg tagataaaga agagcatgtt
    75061 gtaggtctga attgcatagc ttcagcaatt tacgatgatg taggtagtgt tgttgccgct
    75121 atctccatct ccgggccttc atcaagactg acagaagatc gttttgtcag tcagggtgag
    75181 ctggtcagag acaccgcccg cgatatcagc acggcgttgg gactgaaagc acatccatag
    75241 tgtctgtcgc atcccgctct gcggagcggg tttttttgac aaaatttgaa agttggaaaa
    75301 tttttccaat taatagaggt aggaataaaa tggcaaaaat gagagccgtt gacgcggcaa
    75361 tgtatgtgct ggagaaagaa ggtatcacca ccgccttcgg cgttccggga gctgcaatca
    75421 atccgttcta ctcagcgatg cgtaagcacg gcggtattcg tcacattctg gcgcgtcatg
    75481 tggaaggtgc ttcgcacatg gcggaaggtt atacccgcgc aacggcaggg aatatcggcg
    75541 tatgtctggg gacttccggt cctgcgggca cggacatgat caccgcgctc tattccgctt
    75601 ctgctgattc cattcctatt ctgtgcatta ccggccaggc accgcgcgcc cgtctgcata
    75661 aagaagattt tcaggccgta gatattgaag caattgctaa accggtcagc aaaatggcgg
    75721 ttacagttcg tgaagcggcg ctggtgcctc gcgtgctgca acaggcattt cacctgatgc
    75781 gttctggtcg tccgggtccg gtactggtgg atttaccgtt cgacgttcag gttgcggaaa
    75841 tcgagtttga tcctgacatg tacgaaccgc tgccggtcta caaacctgct gccagccgta
    75901 tgcagatcga aaaagctgta gaaatgttaa tccaggccga acgtccggtg attgttgccg
    75961 ggggcggggt aattaatgct gacgcagctg cactgttaca acagtttgct gaactgacca
    76021 gcgttccggt gatcccaacg ctaatgggct ggggctgtat cccggacgat catgaactga
    76081 tggccgggat ggtgggtctg caaaccgcgc atcgttacgg taacgcaacg ctgctggcgt
    76141 ctgacatggt gtttggtatc ggtaaccgtt ttgctaaccg tcataccggc tcggtagaga
    76201 aatacaccga agggcgcaaa atcgttcata ttgatattga gccgacgcaa attggtcgcg
    76261 tgctgtgtcc ggatctcggt attgtctctg atgctaaagc ggcgctgaca ctgctggttg
    76321 aagtggcgca ggagatgcaa aaagcgggtc gtctgccgtg tcgtaaagaa tgggtcgccg
    76381 actgccagca gcgtaaacgc actttgctgc gcaaaaccca cttcgacaac gtgccggtga
    76441 aaccgcagcg cgtgtatgaa gagatgaaca aagcctttgg tcgcgatgtt tgttatgtca
    76501 ccaccattgg tctgtcacaa atcgctgcgg cacaaatgct gcatgtcttt aaagaccgcc
    76561 actggatcaa ctgtggtcag gctggtccgt taggctggac gattccggct gcgctagggg
    76621 tttgtgccgc tgatccgaaa cgcaatgtgg tggcgatttc tggcgacttt gacttccagt
    76681 tcctgattga agagttagct gttggcgcgc agttcaacat tccgtacatc catgtgctgg
    76741 tcaacaacgc ttatctgggg ctgattcgcc agtcgcagcg cgcgtttgat atggactact
    76801 gcgtgcaact cgctttcgag aatatcaact ccagcgaagt gaacggttac ggcgtcgacc
    76861 acgtaaaagt agcggaaggt ttaggttgta aagcgattcg cgtcttcaaa ccggaagata
    76921 ttgcgccagc ctttgaacag gcgaaagcct taatggcgca atatcgggta ccggtagtcg
    76981 tggaagttat tctcgagcgt gtgaccaata tttcgatggg cagcgaactg gataacgtca
    77041 tggaatttga agatatcgcc gataacgcag cggacgcacc gactgaaacc tgcttcatgc
    77101 actatgaata agggagataa ataatgttac gtttctctgc taatttatcg atgttatttg
    77161 gagaatatga ttttctcgcc cgttttgaga aagctgcgca gtgtggtttt cgcggcgttg
    77221 aatttatgtt tccttatgac tacgacattg aagaattaaa acaggtgctg gcgagtaata
    77281 aactcgaaca tacgctgcac aatctaccgg cgggtgactg ggcggcgggg gagcgcggta
    77341 ttgcctgtat tcctggccgt gaagaagagt ttcgggatgg cgtagcagca gcgattcgtt
    77401 atgcccgtgc gctggggaat aaaaaaatta actgtctggt cggtaaaacg ccggctggtt
    77461 tcagcagtga acagattcac gcaacgcttg tagaaaacct gcgttatgcc gcgaatatgc
    77521 tgatgaaaga agatatttta ttactgattg aacctattaa ccattttgat attcctggtt
    77581 tccatctcac cggaactcgg caggcgctga aattgattga tgatgttggt tgctgcaatt
    77641 tgaaaattca gtatgacatt tatcacatgc agcggatgga aggtgaatta accaacacca
    77701 tgactcagtg ggccgataaa attggccacc tgcaaattgc tgataatccg catcgcggcg
    77761 aaccggggac cggagaaatt aattatgatt atctctttaa ggtaatcgaa aattctgact
    77821 acaacggttg ggttgggtgt gaatataaac cccaaaccac cacggaagcc ggtttacgct
    77881 ggatggatcc gtaccgttaa aacgtaacgc tgttcaggca atgctttttt aggccgctaa
    77941 gttggcaggg gatcgtgttg tctgaattca ggaaaagcga aatttaaaag aggttaatta
    78001 tgaaactggg atttattggc ttaggcatta tgggtacacc gatggccatt aatctggcgc
    78061 gtgccggtca tcaactgcat gtcacaacca ttggtccggt tgctgatgaa ttactgtcac
    78121 tgggtgccgt cagtgttgaa actgcgcgcc aggtaacgga agcatcggac atcattttta
    78181 ttatggtgcc agatacacca caggttgaag aagttctgtt cggtgaaaat ggttgtacca
    78241 aagcctcgct gaagggcaaa accattgttg atatgagctc catttccccg attgaaacta
    78301 agcgtttcgc tcgtcaggtg aatgaactgg gcggcgatta tctcgatgcg ccagtctccg
    78361 gcggtgaaat cggtgcgcgt gaagggacgt tgtcgattat ggttggcggt gatgaagcgg
    78421 tatttgaacg tgttaaaccg ctttttgaac tgctcggtaa aaatatcacc ctcgtgggcg
    78481 gtaacggcga cggtcaaacc tgcaaagtgg caaatcagat tatcgtggcg ctcaatattg
    78541 aagcggtttc tgaagccctg ctatttgctt caaaagccgg tgcggacccg gtacgtgtgc
    78601 gccaggcgct gatgggcggc tttgcttcct cacgtattct ggaagttcac ggcgagcgta
    78661 tgattaaacg cacctttaat ccgggcttca aaatcgctct gcaccagaaa gatctcaacc
    78721 tggcactgca aagtgcgaaa gcacttgcgc tgaacctgcc aaacactgcg acctgccagg
    78781 agttatttaa tacctgtgcg gcaaacggtg gcagccagtt ggatcactct gcgttagtgc
    78841 aggcgctgga attaatggct aaccataaac tggcctgata cccgcaataa aaatggccga
    78901 tatcagaaaa tgaatcggcc agcaatatta aaaaagaaag cagccaaaga tgttgcttca
    78961 gtattaaaaa taatattttt attttatttg ttcctcatag ctagattaaa acaacgttat
    79021 tcgatacgtg aaattaagag ggatttatgg aacatcagag aaaactattc cagcaacgcg
    79081 gctatagcga agatctattg ccgaaaacgc aaagccagcg gacctggaaa acatttaact
    79141 attttacctt atggatgggt tcggttcata acgttcccaa ttatgtgatg gtcggcggct
    79201 tttttattct cggcttgtct acctttagta ttatgctggc aattatcctc agcgcctttt
    79261 tcattgccgc ggtaatggta ttaaacggcg ctgcgggcag taaatacggc gtaccgtttg
    79321 ccatgatcct gcgtgcttct tacggcgtac gtggcgcact gtttcccgga ttattaagag
    79381 gcggtattgc tgccattatg tggtttggcc tgcaatgtta cgcggggtca ctggcctgct
    79441 tgattctgat tggcaaaatc tggccgggat ttttaactct cggtggtgat ttcacgctgt
    79501 tagggctttc tctaccgggc ttaattactt tcttactctt ctggctggtc aacgttggta
    79561 taggttttgg cggtggcaaa gttttaaata aattcactgc cattcttaac ccgtgcatct
    79621 atatcgtttt cggcggtatg gcgatttggg cgatttcgct ggtcgggctc ggtccaatct
    79681 ttgactacat tccgagcggt attcagaaag cagaaaacag tggcttcctg ttcctggtgg
    79741 tgataaacgc ggtagttgcg gtctgggcgg caccggcggt gagcgcatcc gactttacgc
    79801 aaaacgccca ctcgtttcgt gagcaagcgc tggggcaaac gctgggttta gttgtggcct
    79861 atattctgtt tgcggtcgcg ggggtatgta ttattgccgg agccagtatt cattacggcg
    79921 ctgatacctg gaacgtgctg gatattgttc agcgttggga cagcttgttc gcctcgttct
    79981 ttgcggtact ggttattctg atgacgacta tctccactaa cgccaccggt aatattattc
    80041 ctgccggtta tcagattgcc gctattgcac cgacgaaact gacttataaa aacggcgtac
    80101 tgattgccag tattatcagc ctgctgatct gcccgtggaa attaatggaa aatcaggaca
    80161 gtatttatct gttcctcgat attatcggcg gaatgcttgg tccggtaatt ggtgtcatga
    80221 tggcgcatta ttttgtggtg atgcgtggga aaattaatct tgatgaactg tataccgcac
    80281 ctggcgatta taaatattac gataacggtt ttaacctcac tgcgttttca gtaactctgg
    80341 tggccgttat tttatctctt ggcggtaagt ttattccctt tatggaaccg ttatcgcgtg
    80401 tttcatggtt tgtcggcgtc atcgtcgcct ttgcggccta cgccttatta aagaaacgta
    80461 caacagcaga aaaaacagga gagcaaaaaa ccataggtta attaatcccg atattgaaca
    80521 ttgagttaaa aaccaatctg tattttacaa ggagtttgtt atgtcttttg atttaatcat
    80581 taaaaacggc accgttattt tagaaaacga agctcgcgtt gtagatatcg ccgttaaagg
    80641 cggaaaaatt gctgctatcg gtcaggatct gggcgatgca aaagaagtta tggatgcgtc
    80701 tggtctggtg gtttcgccgg gcatggttga tgcgcacacc catatttctg aaccgggtcg
    80761 tagccactgg gaaggttatg aaaccggtac tcgcgcagcg gcaaaaggtg gtatcaccac
    80821 catgatcgaa atgccgctca accagctgcc tgcaacggtt gaccgtgctt caattgaact
    80881 gaagttcgat gccgctaaag gcaagctgac tatcgatgcg gcacaactcg gtggcctggt
    80941 gtcttacaac attgatcgtc tgcatgagtt ggatgaagtg ggcgttgtcg gcttcaaatg
    81001 cttcgttgcg acctgtggcg atcgcggtat cgacaacgac ttccgtgatg taaacgactg
    81061 gcagttcttc aaaggtgcgc agaagctggg cgaactgggg cagccggtgt tggtgcactg
    81121 cgaaaacgcg ctgatctgtg acgcactggg cgaagaagcg aaacgtgaag gtcgcgtaac
    81181 tgcccatgac tatgtggctt cgcgtccggt atttaccgaa gtggaagcga ttcgccgcgt
    81241 gctgtatctg gcgaaagttg ccggttgccg tctgcacgtt tgccatatca gcagcccgga
    81301 aggtgttgaa gaagtgactc gtgcacgtca ggaaggtcag gatgttacct gtgaatcctg
    81361 cccgcattac tttgtactgg ataccgatca gttcgaagaa atcggcactc tggcgaagtg
    81421 ttcaccgccg atccgcgatc tggaaaacca gaaaggcatg tgggaaaaac tgtttaacgg
    81481 tgaaatcgac tgcctggttt ccgaccactc accatgccct ccggaaatga aagccggcaa
    81541 catcatggaa gcatggggcg gtattgccgg tctgcaaaac tgtatggacg tgatgttcga
    81601 tgaagcggta cagaaacgcg gaatgtctct gccaatgttc ggcaaattaa tggcgactaa
    81661 cgcagcagat attttcggtc tgcagcaaaa aggccgtatc gccccaggaa aagatgccga
    81721 cttcgtcttc attcagccga atagcagcta tgttcttacc aatgacgatc tggaatatcg
    81781 ccacaaagtc agcccgtatg ttggccgtac cattggcgcg cgtatcacga aaaccatctt
    81841 acgtggtgat gtgatttacg acattgaaca gggcttccct gttgcgccga aaggtcaatt
    81901 tatccttaaa catcagcagt aatctggccc ctgcaatgcc cgtccttgcg gcgggcattc
    81961 tccggttaag gtgtgtttat gttcaatttt gcagtcagcc gcgaaagcct gttatcagga
    82021 tttcagtggt ttttctttat tttttgcaac acggttgtgg ttcctcctac gctactttct
    82081 gcttttcagt tgccgcaaag tagcctgctt acgctcacgc aatatgcttt tcttgctacc
    82141 gcactggcct gcttcgctca ggcgttctgc ggtcatcgtc gcgctattat ggaagggccg
    82201 ggtggcctgt ggtggggaac catccttact atcacccttg gcgaagcatc gcgcgggaca
    82261 ccgatcaacg atatcgctac cagcctggcg gtggggattg cgctctccgg cgtgctgacg
    82321 atgttgattg gttttagcgg attaggccat cgcctggcac ggttatttac gccgtcggtg
    82381 atggtcttgt ttatgttgat gctaggcgcg cagctgacca ctatcttttt caaaggtatg
    82441 ctcggactgc cgtttggcat agccgaccca aattttaaaa ttcagttacc accgttcgcg
    82501 ctctcggtgg cggtgatgtg cctggtactg gggatgatta tcttcctgcc gcaacgtttt
    82561 gcccgttatg gcctgctagt cggcaccata accggctggt tgttgtggta tttttgcttt
    82621 ccttcttcgc actcgctctc cggtgagttg cactggcagt ggttcccgct cggcagtggc
    82681 ggtgctttgt cgccggggat tattctgacg gcggtgatta caggtctggt aaatatcagc
    82741 aatacctacg gtgcgattcg gggcacggat gttttttatc cgcagcaggg cgcggggaat
    82801 acgcgttatc gtcgtagctt tgtggcgacc ggatttatga cactgataac cgtaccgctg
    82861 gcagtaattc cattttcacc gtttgtttca tccattggtt tattaaccca gactggcgat
    82921 tacacgcggc gttcgtttat ttatggcagc gttatttgcc tgctggtggc gctggttccg
    82981 gcactcacgc gactgttttg cagtatccct ttacccgtga gtagcgcggt catgctggtt
    83041 tcttatctgc ctttactctt ttccgcgctg gtgtttagcc agcaaataac gtttaccgct
    83101 cgcaatattt atcgactcgc attgccgtta tttgtcggca tatttttaat ggcattaccg
    83161 cctgtgtatc tgcaagacct tccattaacg cttcgtcctc tgctcagtaa cggcttattg
    83221 gtcgggattt tactggctgt tcttatggat aaccttattc cgtgggaacg tatcgaataa
    83281 tttgttgaaa aaggattgat aatgaagatt gtcattgcgc cagactcttt taaagagagc
    83341 ttaagtgcag aaaaatgttg tcaggcaatt aaagccgggt tttcgaccct ctttcccgat
    83401 gcgaactata tctgtttgcc gatagcggat ggcggcgaag gaacggtgga tgcgatggtc
    83461 gccgcgacgg gcggcaacat cgtgacgctt gaagtctgcg ggccgatggg cgaaaaagtg
    83521 aatgcttttt atggccttac cggcgacggg aaaacggcgg tgattgagat ggcggcagca
    83581 agtggcctga tgctggtcgc gcctgaaaag cgtaatccgt tgctggcctc cagttttggt
    83641 acgggggagt taattcgtca tgcgctggat aacggcattc gccatattat tctcggcatt
    83701 ggcggcagtg cgacggtcga cggcggtatg ggcatggcgc aagcgcttgg tgtgcgtttt
    83761 ctcgatgccg acggtcaggt gctggcagct aacggcggca atttagcgcg cgtggcaagc
    83821 attgagatgg aggaatgcga tccgcgtctg gcgaacagcc atattgaagt agcatgtgac
    83881 gttgataacc cgctggtagg ggcacgcggc gcggcggcgg tgtttggccc gcaaaaaggg
    83941 gcaacgccgg agatggtcga agagctggaa caggggctgc aaaattacgc ccgtgtttta
    84001 caacagcaaa ctgaaattaa tgtctgtcag atggcgggcg gcggcgctgc gggcggtatg
    84061 ggtattgcgg cggcggtatt tctcaatgcg gatattaaac cgggcattga aattgtgttg
    84121 aatgcagtca atcttgcgca ggcagtgcag ggcgcagcac tggtgatcac cggggaaggg
    84181 cgcatcgact cgcaaacggc aggcggtaaa gcgccgctgg gtgtggcgtc ggtggcgaaa
    84241 cagtttaatg taccggtgat tggaattgct ggcgtattgg gtgatggcgt ggaagtggta
    84301 caccagtacg gcattgacgc ggtattcagc attttgccac gtctggcacc tttagccgaa
    84361 gtgctcgcca gcggtgaaac caatctcttc aacagcgcgc gaaatattgc ctgcgccatt
    84421 aaaataggtc agggaattaa aaactgactc ttgcctttaa agcggatgct atttatttcg
    84481 tataagagcg cagtactcat gccggatgcg gcatgagtac tatatccttc ctgaaaatcg
    84541 cgcaaattct atatattgca gagatcatgt aggcctgata agcgaagcgc atcaggcaat
    84601 gttacaaaaa aagccacggt ataaaccgtg gcaaaatcca acatagctaa aaataatcag
    84661 gcgagtggta tgacttaaat ctctacgtcg cggttgcaat ctttcgagta aatatagctg
    84721 aacgcttcac cacgccctac gccataacca gcctgtaaag aataagcgcc cataaagatg
    84781 taatcgcctt ttttcaccgg gatccagtta ttgtcgaggt tataaacccc ctgaccggaa
    84841 agaatatagg caccatgttc ctgaacgtgt gtttcgatat aaccgtggct ggcacctggc
    84901 gcaaaagaga ggatatgcat gttcatatca aaacctaact ctttgggcag gaaatccagc
    84961 aggatcacat catccatacc ttcatagtga atgcgttcca gttcgctggc attaccagaa
    85021 accagccacg gtgcatggcc ttctaccgga acatagcggc gcttatataa aaagatttgg
    85081 ctgtcttcgg cctgggcgtt aacaaacgtc attaaggagc ctggtgggca ataaagatag
    85141 ccaccttcgc ttaaggcaaa tgttttgcct tcggctttgg cagtgatatt tccagagatc
    85201 acatacagga acgtttcaat gccttcgcca ccgaagccct gttggttgcc accgttttga
    85261 tgcagtgtga ccagataatc aacaaaagag gcacccagct ttggtgtgga gaggattgtc
    85321 gcgtcacaat tttcaaagcc cggaataata ttttttacca gaccgtctgg tgttaacagt
    85381 gcgaaattac cgtgtttcac aatcgcacgg ttagccagta aatcatcgcg gtaaccggtg
    85441 acgttattta aatatcccat ttataactcc ttatttctgc caggcaagtt gataaagcat
    85501 gagtgccaac gttttgaccc cttcggcaag gtcggtaata ttggtgcgtt ccgccgggtt
    85561 atggctgatc ccgttgatgc tggggataaa aatcatgcag gttggtacgc gaggcgcgaa
    85621 aatttgcgcg tcgtgcccgg caccattgtg catcacccgg taattcagtt tttcgctttc
    85681 acacaattct gtcagggtgg cgaccagctc cttattcatc ggcacgggtt cttcgtccat
    85741 ccataaatcg atatcaatac caatgtccat ttcatcgcaa atcgcccgca tgtcgttttc
    85801 taactgttgg gtgaaatcgc gcagcacggc agcgtcggta tgacgacaat caatggtgaa
    85861 cgtggtttta cccggcacca catttaccgt attcgggcgc ggctctactt tgccaaaggt
    85921 cagaaccagc ggatcgccca tccttttcgc tttttcgacc gactgatggc aaatgcgact
    85981 gaaagcgtaa actgtatcac gacgataacc catcggcgtg gtgcctgcat ggtttgattc
    86041 gccgttcagc gttaccgtat aacgacgctg cccgacaatt gcattcacca cgccaattga
    86101 ttgcccatta ctttccagca cacagccctg ttcaatatgc agttcaacaa aggctttaat
    86161 atcctgacgc ggagttagtg gggcgttcgg aagagtaaat ccgcaagcct tcatcgcatc
    86221 gacaaaacta tttcctttgg catcacagat attccgcacg tcgtcaggat tcgccagccc
    86281 aaagatattt ttactgcccc agaagacata cgggaagcgg ctgccttctt cttctgccat
    86341 cgccaccact tcgaccgtac gtagcggcgc gccgtattgc gttttcagcc agtcaattgc
    86401 cagccacgcc gccagcgcgc cgaattgccc gtcgaggtta ccgccgttaa ccacggtatc
    86461 gatatgcgaa ccgctcagaa ccacttcttg tggatattcg gtgccactca ggcgaccata
    86521 taaattcccc acttcatcga aacgtgtttc cagcccgctt gctgccattc tttttttaaa
    86581 ttgctgctga gtttccagcc attccggcga ataaagtaaa cgggtcatac cacccgttgg
    86641 gtcagcgcca aaagaggaaa gccagggcag cgtttcttct atggcttgac gaaaatgtgt
    86701 aatcataaga aagtcctgtc tcaataatta ttgcgcaaag ggatttttcg tttcgtatga
    86761 cgtgttataa agcgcgtcgg aaattaaata ctggtaaata tcatcaacaa tttcgatgcc
    86821 ttcgacggcg gctttgcgtt gtttaatatc ctgatcctgt ccgggataat aaacctgatt
    86881 aaaaccgggc gcgggggtaa tggcatttaa ttcgcgcatg gtctggctaa gatgttgacg
    86941 gaataattcg ctggaggaga aaaagttcgg gttaataact acatgtaatt gccccaaatt
    87001 acgcccggcg tgtaaatcgt catacatcga actaacctgt cgcccgaacg gtaagccgag
    87061 taagacgcct gagaggacgt caatcatcat catcaggcca taccctttcg gcccggcggc
    87121 ggggagcaga gcatgtaccg cgaacggatc ggttgttggt gcaccgtttt tatcgaccgc
    87181 ccaagtatcc gggatagaca tattacgcga gcgggcgtcg agcacttttc cccatgcctg
    87241 tacggtagtc gccatatcaa aggtaaggat ctcgttgcct tctcccggcg cggcaaaggc
    87301 cagtgggtta gtaccgtagt aaatttccgc gccgccaaac ggcaccacca ttggatcgga
    87361 ctggcacatc gaaatgccaa ttaatccggc gcgggctgcc tgctgcacaa aataagagat
    87421 tgcgccgctg tgacccatcc ggctgatacc gaccaccgca acgccatttt gctgggcggt
    87481 tttgatggca tgttccatac ccattttcgc cgcgacctgt ccggcggcat tgtcggcatg
    87541 taaaattgcc gagcacggcc cggtttcctc aagacgaaac tccggttcgc ggttggtacc
    87601 gccttttgaa atgcgttccg cgtagtattc cacgcgcacc gcgccatgag agtggatccc
    87661 tctggcatcg gcgtaaacca atacttcagc cacggttgca gcgtgctcac gttttaaccc
    87721 agcctggcag agtttattct caattagctg gtggagtgtt tcccgactga ttttcatctg
    87781 tcttcctttt taacgacggt gtgaagcatg actgcaatta acatacaggg aaaatatctg
    87841 gattatgtga tccagacagg caaaaaaata tagttagaat ttatttgata atccgctcgc
    87901 ttttaacctg gtttttaaaa ca
//
LOCUS       DBANUU010000002        80423 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000002 DBANUU010000000
VERSION     DBANUU010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.002"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..577
                     /gene="yraH"
                     /locus_tag="ACIE68_000082"
     CDS             <1..577
                     /gene="yraH"
                     /locus_tag="ACIE68_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5906461.1"
                     /translation="VTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSDKKI
                     EVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRDNGT
                     VPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGIVNV
                     DLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            657..1352
                     /gene="yraI"
                     /locus_tag="ACIE68_000083"
     CDS             657..1352
                     /gene="yraI"
                     /locus_tag="ACIE68_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFX5906462.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDNVNANELTLVFKTRIKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1382..3898
                     /gene="yraJ"
                     /locus_tag="ACIE68_000084"
     CDS             1382..3898
                     /gene="yraJ"
                     /locus_tag="ACIE68_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFX5906463.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYSGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSVNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3909..5000
                     /gene="yraK"
                     /locus_tag="ACIE68_000085"
     CDS             3909..5000
                     /gene="yraK"
                     /locus_tag="ACIE68_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5906464.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLKANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5043..5903)
                     /gene="rsmI"
                     /locus_tag="ACIE68_000086"
     CDS             complement(5043..5903)
                     /gene="rsmI"
                     /locus_tag="ACIE68_000086"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFX5906465.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5968..8004
                     /gene="lpoA"
                     /locus_tag="ACIE68_000087"
     CDS             5968..8004
                     /gene="lpoA"
                     /locus_tag="ACIE68_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFX5906466.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7962..8357
                     /gene="yraN"
                     /locus_tag="ACIE68_000088"
     CDS             7962..8357
                     /gene="yraN"
                     /locus_tag="ACIE68_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFX5906467.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8377..8967
                     /gene="diaA"
                     /locus_tag="ACIE68_000089"
     CDS             8377..8967
                     /gene="diaA"
                     /locus_tag="ACIE68_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFX5906468.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHTGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            8977..9552
                     /gene="dolP"
                     /locus_tag="ACIE68_000090"
     CDS             8977..9552
                     /gene="dolP"
                     /locus_tag="ACIE68_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFX5906469.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9757..10797)
                     /gene="yraQ"
                     /locus_tag="ACIE68_000091"
     CDS             complement(9757..10797)
                     /gene="yraQ"
                     /locus_tag="ACIE68_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFX5906470.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWEPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGTVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10870..11505)
                     /gene="yraR"
                     /locus_tag="ACIE68_000092"
     CDS             complement(10870..11505)
                     /gene="yraR"
                     /locus_tag="ACIE68_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFX5906471.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAESMRPEHEGVTILSSSELRKRAE"
     gene            11633..12151
                     /gene="yhbO"
                     /locus_tag="ACIE68_000093"
     CDS             11633..12151
                     /gene="yhbO"
                     /locus_tag="ACIE68_000093"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFX5906472.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12131..12574)
                     /gene="yhbP"
                     /locus_tag="ACIE68_000094"
     CDS             complement(12131..12574)
                     /gene="yhbP"
                     /locus_tag="ACIE68_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFX5906473.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            12625..12927
                     /gene="yhbQ"
                     /locus_tag="ACIE68_000095"
     CDS             12625..12927
                     /gene="yhbQ"
                     /locus_tag="ACIE68_000095"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFX5906474.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12914..13417)
                     /gene="yhbS"
                     /locus_tag="ACIE68_000096"
     CDS             complement(12914..13417)
                     /gene="yhbS"
                     /locus_tag="ACIE68_000096"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFX5906475.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13411..13935)
                     /gene="ubiT"
                     /locus_tag="ACIE68_000097"
     CDS             complement(13411..13935)
                     /gene="ubiT"
                     /locus_tag="ACIE68_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFX5906476.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14144..15139
                     /gene="ubiU"
                     /locus_tag="ACIE68_000098"
     CDS             14144..15139
                     /gene="ubiU"
                     /locus_tag="ACIE68_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFX5906477.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15148..16026
                     /gene="ubiV"
                     /locus_tag="ACIE68_000099"
     CDS             15148..16026
                     /gene="ubiV"
                     /locus_tag="ACIE68_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFX5906478.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16232..17239
                     /gene="yhbW"
                     /locus_tag="ACIE68_000100"
     CDS             16232..17239
                     /gene="yhbW"
                     /locus_tag="ACIE68_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFX5906479.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPNVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17357..18601)
                     /gene="mtr"
                     /locus_tag="ACIE68_000101"
     CDS             complement(17357..18601)
                     /gene="mtr"
                     /locus_tag="ACIE68_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFX5906480.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18755..20644)
                     /gene="deaD"
                     /locus_tag="ACIE68_000102"
     CDS             complement(18755..20644)
                     /gene="deaD"
                     /locus_tag="ACIE68_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFX5906481.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20637..20717)
                     /gene="yrbN"
                     /locus_tag="ACIE68_000103"
     CDS             complement(20637..20717)
                     /gene="yrbN"
                     /locus_tag="ACIE68_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFX5906482.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20824..21708)
                     /gene="nlpI"
                     /locus_tag="ACIE68_000104"
     CDS             complement(20824..21708)
                     /gene="nlpI"
                     /locus_tag="ACIE68_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFX5906483.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21817..23952)
                     /gene="pnp"
                     /locus_tag="ACIE68_000105"
     CDS             complement(21817..23952)
                     /gene="pnp"
                     /locus_tag="ACIE68_000105"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFX5906484.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24199..24468)
                     /gene="rpsO"
                     /locus_tag="ACIE68_000106"
     CDS             complement(24199..24468)
                     /gene="rpsO"
                     /locus_tag="ACIE68_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFX5906485.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24617..25561)
                     /gene="truB"
                     /locus_tag="ACIE68_000107"
     CDS             complement(24617..25561)
                     /gene="truB"
                     /locus_tag="ACIE68_000107"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFX5906486.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25561..25962)
                     /gene="rbfA"
                     /locus_tag="ACIE68_000108"
     CDS             complement(25561..25962)
                     /gene="rbfA"
                     /locus_tag="ACIE68_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFX5906487.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(26126..28798)
                     /gene="infB"
                     /locus_tag="ACIE68_000109"
     CDS             complement(26126..28798)
                     /gene="infB"
                     /locus_tag="ACIE68_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFX5906488.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKRKAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28823..30310)
                     /gene="nusA"
                     /locus_tag="ACIE68_000110"
     CDS             complement(28823..30310)
                     /gene="nusA"
                     /locus_tag="ACIE68_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFX5906489.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30338..30790)
                     /gene="rimP"
                     /locus_tag="ACIE68_000111"
     CDS             complement(30338..30790)
                     /gene="rimP"
                     /locus_tag="ACIE68_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFX5906490.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30997..31073)
                     /locus_tag="ACIE68_000112"
     tRNA            complement(30997..31073)
                     /locus_tag="ACIE68_000112"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31037..31039),aa:Met,seq:cat)
     gene            31421..32764
                     /gene="argG"
                     /locus_tag="ACIE68_000113"
     CDS             31421..32764
                     /gene="argG"
                     /locus_tag="ACIE68_000113"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFX5906491.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(32772..34397)
                     /gene="yhbX"
                     /locus_tag="ACIE68_000114"
     CDS             complement(32772..34397)
                     /gene="yhbX"
                     /locus_tag="ACIE68_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFX5906492.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVILFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34856..34942)
                     /locus_tag="ACIE68_000115"
     tRNA            complement(34856..34942)
                     /locus_tag="ACIE68_000115"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34906..34908),aa:Leu,seq:gag)
     gene            complement(34957..35289)
                     /gene="secG"
                     /locus_tag="ACIE68_000116"
     CDS             complement(34957..35289)
                     /gene="secG"
                     /locus_tag="ACIE68_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFX5906493.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(35517..36854)
                     /gene="glmM"
                     /locus_tag="ACIE68_000117"
     CDS             complement(35517..36854)
                     /gene="glmM"
                     /locus_tag="ACIE68_000117"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFX5906494.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36859..37695)
                     /gene="folP"
                     /locus_tag="ACIE68_000118"
     CDS             complement(36859..37695)
                     /gene="folP"
                     /locus_tag="ACIE68_000118"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFX5906495.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     EN"
     gene            complement(37785..39719)
                     /gene="ftsH"
                     /locus_tag="ACIE68_000119"
     CDS             complement(37785..39719)
                     /gene="ftsH"
                     /locus_tag="ACIE68_000119"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFX5906496.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39819..40448)
                     /gene="rlmE"
                     /locus_tag="ACIE68_000120"
     CDS             complement(39819..40448)
                     /gene="rlmE"
                     /locus_tag="ACIE68_000120"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFX5906497.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40574..40867
                     /gene="yhbY"
                     /locus_tag="ACIE68_000121"
     CDS             40574..40867
                     /gene="yhbY"
                     /locus_tag="ACIE68_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFX5906498.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(41023..41499)
                     /gene="greA"
                     /locus_tag="ACIE68_000122"
     CDS             complement(41023..41499)
                     /gene="greA"
                     /locus_tag="ACIE68_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFX5906499.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41747..43180
                     /gene="dacB"
                     /locus_tag="ACIE68_000123"
     CDS             41747..43180
                     /gene="dacB"
                     /locus_tag="ACIE68_000123"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFX5906500.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(43366..44538)
                     /gene="cgtA"
                     /locus_tag="ACIE68_000124"
     CDS             complement(43366..44538)
                     /gene="cgtA"
                     /locus_tag="ACIE68_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFX5906501.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(44554..45519)
                     /gene="yhbE"
                     /locus_tag="ACIE68_000125"
     CDS             complement(44554..45519)
                     /gene="yhbE"
                     /locus_tag="ACIE68_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFX5906502.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(45646..45903)
                     /gene="rpmA"
                     /locus_tag="ACIE68_000126"
     CDS             complement(45646..45903)
                     /gene="rpmA"
                     /locus_tag="ACIE68_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFX5906503.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45924..46235)
                     /gene="rplU"
                     /locus_tag="ACIE68_000127"
     CDS             complement(45924..46235)
                     /gene="rplU"
                     /locus_tag="ACIE68_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFX5906504.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            46494..47465
                     /gene="ispB"
                     /locus_tag="ACIE68_000128"
     CDS             46494..47465
                     /gene="ispB"
                     /locus_tag="ACIE68_000128"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFX5906505.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            47692..47970
                     /gene="sfsB"
                     /locus_tag="ACIE68_000129"
     CDS             47692..47970
                     /gene="sfsB"
                     /locus_tag="ACIE68_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFX5906506.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(48018..49277)
                     /gene="murA"
                     /locus_tag="ACIE68_000130"
     CDS             complement(48018..49277)
                     /gene="murA"
                     /locus_tag="ACIE68_000130"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFX5906507.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(49332..49586)
                     /gene="ibaG"
                     /locus_tag="ACIE68_000131"
     CDS             complement(49332..49586)
                     /gene="ibaG"
                     /locus_tag="ACIE68_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFX5906508.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49746..50039)
                     /gene="mlaB"
                     /locus_tag="ACIE68_000132"
     CDS             complement(49746..50039)
                     /gene="mlaB"
                     /locus_tag="ACIE68_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFX5906509.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(50039..50674)
                     /gene="mlaC"
                     /locus_tag="ACIE68_000133"
     CDS             complement(50039..50674)
                     /gene="mlaC"
                     /locus_tag="ACIE68_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFX5906510.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50693..51244)
                     /gene="mlaD"
                     /locus_tag="ACIE68_000134"
     CDS             complement(50693..51244)
                     /gene="mlaD"
                     /locus_tag="ACIE68_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFX5906511.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(51249..52031)
                     /gene="mlaE"
                     /locus_tag="ACIE68_000135"
     CDS             complement(51249..52031)
                     /gene="mlaE"
                     /locus_tag="ACIE68_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFX5906512.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(52039..52848)
                     /gene="mlaF"
                     /locus_tag="ACIE68_000136"
     CDS             complement(52039..52848)
                     /gene="mlaF"
                     /locus_tag="ACIE68_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFX5906513.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            53058..54035
                     /gene="yrbG"
                     /locus_tag="ACIE68_000137"
     CDS             53058..54035
                     /gene="yrbG"
                     /locus_tag="ACIE68_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFX5906514.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYRLSPILVE"
     gene            54049..55035
                     /gene="kdsD"
                     /locus_tag="ACIE68_000138"
     CDS             54049..55035
                     /gene="kdsD"
                     /locus_tag="ACIE68_000138"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFX5906515.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            55056..55622
                     /gene="kdsC"
                     /locus_tag="ACIE68_000139"
     CDS             55056..55622
                     /gene="kdsC"
                     /locus_tag="ACIE68_000139"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFX5906516.1"
                     /translation="MSKAGASLATCYGSVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            55619..56194
                     /gene="lptC"
                     /locus_tag="ACIE68_000140"
     CDS             55619..56194
                     /gene="lptC"
                     /locus_tag="ACIE68_000140"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFX5906517.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            56163..56720
                     /gene="lptA"
                     /locus_tag="ACIE68_000141"
     CDS             56163..56720
                     /gene="lptA"
                     /locus_tag="ACIE68_000141"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFX5906518.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            56727..57452
                     /gene="lptB"
                     /locus_tag="ACIE68_000142"
     CDS             56727..57452
                     /gene="lptB"
                     /locus_tag="ACIE68_000142"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFX5906519.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            57500..58933
                     /gene="rpoN"
                     /locus_tag="ACIE68_000143"
     CDS             57500..58933
                     /gene="rpoN"
                     /locus_tag="ACIE68_000143"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFX5906520.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            58956..59243
                     /gene="hpf"
                     /locus_tag="ACIE68_000144"
     CDS             58956..59243
                     /gene="hpf"
                     /locus_tag="ACIE68_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFX5906521.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            59361..59852
                     /gene="ptsN"
                     /locus_tag="ACIE68_000145"
     CDS             59361..59852
                     /gene="ptsN"
                     /locus_tag="ACIE68_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFX5906522.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            59898..60752
                     /gene="rapZ"
                     /locus_tag="ACIE68_000146"
     CDS             59898..60752
                     /gene="rapZ"
                     /locus_tag="ACIE68_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFX5906523.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            60749..61021
                     /gene="npr"
                     /locus_tag="ACIE68_000147"
     CDS             60749..61021
                     /gene="npr"
                     /locus_tag="ACIE68_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFX5906524.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            61235..61867
                     /gene="yrbL"
                     /locus_tag="ACIE68_000148"
     CDS             61235..61867
                     /gene="yrbL"
                     /locus_tag="ACIE68_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFX5906525.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(61864..62592)
                     /gene="mtgA"
                     /locus_tag="ACIE68_000149"
     CDS             complement(61864..62592)
                     /gene="mtgA"
                     /locus_tag="ACIE68_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFX5906526.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(62589..63242)
                     /gene="elbB"
                     /locus_tag="ACIE68_000150"
     CDS             complement(62589..63242)
                     /gene="elbB"
                     /locus_tag="ACIE68_000150"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFX5906527.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(63472..65808)
                     /gene="arcB"
                     /locus_tag="ACIE68_000151"
     CDS             complement(63472..65808)
                     /gene="arcB"
                     /locus_tag="ACIE68_000151"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFX5906528.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(65904..66833)
                     /gene="yhcC"
                     /locus_tag="ACIE68_000152"
     CDS             complement(65904..66833)
                     /gene="yhcC"
                     /locus_tag="ACIE68_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFX5906529.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            67508..71968
                     /gene="gltB"
                     /locus_tag="ACIE68_000153"
     CDS             67508..71968
                     /gene="gltB"
                     /locus_tag="ACIE68_000153"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFX5906530.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPHIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            71981..73399
                     /gene="gltD"
                     /locus_tag="ACIE68_000154"
     CDS             71981..73399
                     /gene="gltD"
                     /locus_tag="ACIE68_000154"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFX5906531.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            73583..74710
                     /gene="yhcG"
                     /locus_tag="ACIE68_000155"
     CDS             73583..74710
                     /gene="yhcG"
                     /locus_tag="ACIE68_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFX5906532.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(74770..75234)
                     /gene="nanQ"
                     /locus_tag="ACIE68_000156"
     CDS             complement(74770..75234)
                     /gene="nanQ"
                     /locus_tag="ACIE68_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFX5906533.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(75231..76106)
                     /gene="nanK"
                     /locus_tag="ACIE68_000157"
     CDS             complement(75231..76106)
                     /gene="nanK"
                     /locus_tag="ACIE68_000157"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFX5906534.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(76103..76792)
                     /gene="nanE"
                     /locus_tag="ACIE68_000158"
     CDS             complement(76103..76792)
                     /gene="nanE"
                     /locus_tag="ACIE68_000158"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFX5906535.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(76840..78330)
                     /gene="nanT"
                     /locus_tag="ACIE68_000159"
     CDS             complement(76840..78330)
                     /gene="nanT"
                     /locus_tag="ACIE68_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFX5906536.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(78439..79332)
                     /gene="nanA"
                     /locus_tag="ACIE68_000160"
     CDS             complement(78439..79332)
                     /gene="nanA"
                     /locus_tag="ACIE68_000160"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFX5906537.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(79454..80245)
                     /gene="nanR"
                     /locus_tag="ACIE68_000161"
     CDS             complement(79454..80245)
                     /gene="nanR"
                     /locus_tag="ACIE68_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFX5906538.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        19802 a        21733 c        19962 g        18926 t
ORIGIN      
        1 agttacaaaa acagctattg cgggcttact tgcgcttttc gcaggtaatg ccgctgcaac
       61 cgatggtgaa atagtttttg atggcgagat tttaaaatcc gcttgtgaaa tcaatgactc
      121 tgataagaaa attgaagttg ctcttggtca ctataatgcc gaacagtttc gttctgtagg
      181 cgatcgtagc ccaaaaattc cattcactat tcctttagtt aactgcccag tgactgggtg
      241 ggagcacgat aacggcaacg ttgaagcgtc tttccgttta tggctggaaa cgcgggataa
      301 tggtaccgtc cctaacttcc ctaacttggc aaaagtgggc tcttttgctg gcacagcagc
      361 aacaggtgtg ggtatccgta ttgacgatgc ggaaagcgga aaccttatgc cactaaatgc
      421 tatgggcaat gataacacag tttatcagat ccctgcggac tccgctggaa ttgtcaatgt
      481 tgacctcatc gcttactacg tatcaaccgt tgaagcatca gaaatcaccc caggggaagc
      541 agacgctgtc gttaacgtaa cgctggatta ccgttaatac gttacggcgt tatctgacct
      601 ttcagataac gcccttttcc ttcctctttc tcgttgtatc aggttgaaaa ataactatgt
      661 caaaacgaac attcgcggtg ataataacct tgttgtgtag cttctgtatc ggccaggcgc
      721 ttgcaggggg tatcgtttta cagcgaacgc gagtgatcta tgatgccagc cgcaaagagg
      781 ctgcgttacc tgtcgcaaac aaaggcgcag aaacgcctta tttactgcaa tcatgggtag
      841 ataatataga tggtacaagc cgtgccccat ttattataac cccaccgcta tttcgtcttg
      901 aagctggcga tgattcatca ctgcgaatta ttaaaacagc tgataacctg cctgaaaata
      961 aagagtcgct gttctacatt aatgttcgtg ccattccagc aaagaaaaaa tcagataatg
     1021 ttaatgctaa cgagttgacg ctggtattta aaacacggat caaaatgttt tatcgccccg
     1081 cacacctgaa gggacgggta aacgatgcgt ggaaatcact ggaatttaaa cgtagtgacc
     1141 attcactcaa tatatataac ccaactgaat attacgtcgt atttgccgga ctggcagtcg
     1201 ataaaaccga tctcacaagc aaaattgaat atatcgcacc cggagaacat aaacagttac
     1261 cacttcctgc atctggcgga aagaacgtga aatgggctgc gatcaatgat tatggcggca
     1321 gttccgggac agaaactcgt ccactgcaat aacaaatata aaaaacacag gtcatcaggg
     1381 aatgccacaa cgacaccacc agggacataa acgcacaccg aaacagttgg cgctcattat
     1441 caaacgctgt ttgccgatgg tgctcactgg cagcggcatg ctttgcacta ccgctaacgc
     1501 cgaagagtat tatttcgacc ccattatgct ggaaaccaca aaaagtggta tgcaaacaac
     1561 cgatctgtca cgattttcaa agaaatacgc acaactacca ggaacttatc aggttgatat
     1621 ctggctgaat aaaaagaagg tttcacagaa aaaaattaca tttaccgcca atgccgagca
     1681 acttctgcag ccacagttta cggttgaaca actacgtgag ctgggtatta aggtggatga
     1741 aatcccggcg ctggctgaaa aagatgacga tagcgtgatc aactcgcttg aacaaatcat
     1801 tcccggtaca gctgctgaat ttgatttcaa tcatcagcga cttaatttga gcattcccca
     1861 aattgcactg taccgtgatg caagaggtta cgtctcccct tctcgttggg acgatggtat
     1921 accaacgctg tttaccaact actcgtttac aggttctgat aaccgttacc gccagggcaa
     1981 tcgtagccaa cgacagtacc tgaatatgca aaatggtgct aattttggcc cctggcgatt
     2041 acgcaactat tccacatgga cacgcaacga tcagacgtca agctggaaca ctatcagtag
     2101 ttatttacaa cgtgatatca aggcgttgaa atctcagttg cttctgggag aaagcgccac
     2161 cagcggcagt attttttcca gctacacctt tactggcgtg caactcgctt ccgacgataa
     2221 tatgttgcca aacagccagc gcggatttgc cccaacggta cgcggtatcg caaacagtag
     2281 tgcaatcgtg actatcaggc aaaatggtta tgtgatctat caaagcaacg tgccagcggg
     2341 tgcctttgaa attaacgatc tctacccctc ttccaacagc ggcgatttag aagtcacgat
     2401 tgaagaaagt gacggtacgc aacgtcgctt tatccagcct tattcttcat tacccatgat
     2461 gcagcgacct gggcatctaa aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa
     2521 cagtgatagc aaggaacccg aatttgctga agccacggca atatatggtt tgaataatac
     2581 ttttacgctg tatagtggcc tgctcggttc tgaagattat tatgcgctgg ggatcggtat
     2641 cggcggcaca cttggcgcac tgggcgcgtt gtcgatggat atcaacagag ctgacaccca
     2701 attcgataac cagcactctt ttcatggcta tcaatggcgt acgcagtaca tcaaagatat
     2761 cccggaaacc aacaccaata tcgctgtcag ctactatcgc tataccaacg atggctattt
     2821 tagttttgat gaagccaata cccgcaattg ggactataac agtcgccaaa aaagtgaaat
     2881 tcaattcaac atcagccaga caatatttga tggggtaagt ctgtatgcct ccggttcaca
     2941 gcaagactat tggggcaata acgagaaaaa caggaatatc tctgttgggg tttccggcca
     3001 gcaatgggga attggttaca gcctgaatta tcaatacagc cgctacactg atcaaaataa
     3061 tgaccgtgca ctctctttga atctcagtat tccgttagaa cgctggttac cgcgtagccg
     3121 ggtttcctat cagatgacca gccagaaaga tcgcccaacc caacatgaaa tgcgtcttga
     3181 tggttcactg ctggatgatg gtcgcctgag ctatagcctg gaacaaagtc tggatgacga
     3241 taacaaccat aacagtagcg tgaacgccag ttaccgttca ccttatggaa ccttcagtgc
     3301 cggatacagt tacggtaatg acagtagcca atacaattac ggcgttaccg gcggcgtggt
     3361 tatccatcct catggtgtga cgctctcgca atatctgggc aacgcttttg cgcttattga
     3421 tgctaacggg gcatctggcg tgaggataca aaactatccg gggattgcta ctgatccctt
     3481 tggctatgca gtggttcctt atctcacgac ttatcaggaa aaccgtctct cggtagatac
     3541 tacgcagctg cccgataacg tcgatcttga gcaaacaaca cagtttgtgg tgcccaacag
     3601 aggtgcaatg gtagcggcgc gtttcaacgc caatatcggt tatcgcgtac ttgttacagt
     3661 cagcgatcgc aacggtaaac cgttgccctt tggcgctctt gccagcaacg atgagacggg
     3721 gcaacaaagt atcgtcgatg agggcggcat actatatctc tctgggatat cgagtaaatc
     3781 acaaagctgg actgtacgct ggggaaatca ggcagatcaa caatgtcagt ttgcttttag
     3841 tacaccggat tcagaaccaa caacctctgt attacaaggc acagcgcagt gccattaagg
     3901 ataaaaaaat gaaaagagcg cctcttataa caggactttt gttgatatcc acatcctgcg
     3961 cttatgcctc ctcaggaggg tgtggagccg acagcactag cggtgcgaca aattacagca
     4021 gtgtggttga tgatgttacg gtgaaccaga cagataacgt gacaggacgg gagtttacct
     4081 ctgcaacgct aagtagcact aactggcaat acgcctgttc ctgctctgcg ggtaaggcag
     4141 ttaaacttgt ctatatggtc agccctgtac tcaccaccac tggacatcag gcaggatatt
     4201 acaaactcaa tgatagcctg gatattaaaa ccaccttaaa ggcgaatgac attccaggac
     4261 tcgtgactga ccaaaccgtt tctgttaaca cccgattcac acagataaaa agcaacaccg
     4321 tatattctgc tgcaacccaa acgggtgttt gccagggtga cacgtctcgt tatggacccg
     4381 ttaatattgg tgcaaatacc acctttaccc tgtatgtcac caagccattt ctcggctcga
     4441 tgaccattcc gaaaacggat attgccgtca ttaaaggcgc gtgggtcgat ggaatgggaa
     4501 gcccgtctac aggtgacttc catgatttag tcaagttatc gattcaagga aatctcaccg
     4561 ccccgcagtc gtgcaaaatt aatcagggcg atgttattaa ggttaatttt ggattcatca
     4621 atggtcagaa gtttaccacc cgcaatgcca tgccagacgg ttttactcca gtagactttg
     4681 atatcactta tgactgtggt gatacttcaa agattaaaaa ctcgttgcaa atgcgcatcg
     4741 acggtacaac tggggtagta gaccagtaca accttgtcgc cagacgaaga agttcagaca
     4801 atgcgcccga tgtcggtatt cgtattgaaa atctcggcgg cggagttgca aatattcctt
     4861 ttcagaacgg tatccttccc gttgatcctt ccgggcatgg caccgtcaat atgcgcgcct
     4921 ggccagttaa tctggtcggt ggtgagctgg aaacaggaaa atttcagggc acagccacca
     4981 ttaccgtcat cgtgcggtaa aagaagacgt tgcgttttga ttaacgcaac gcttcggcac
     5041 tcttacccct gctgctccag cgcatactta tacagcgcat ttttcttcac gccatgaatc
     5101 tctgcggcca gcgccgccgc ttttttcagc ggtagttctg cctgtagcag cgccagcgtg
     5161 cgcagggcat cggcgggtaa gtcttcttcc tgcgctttat ggccttcgac aatcagcacc
     5221 atttcgcctt tgcgacggtt ttcatcttct ttgacccacg ccagcagctc gccaacgggc
     5281 gcgccgtgaa tggtttccca ggttttggtg agctcacgcg ccagaaccac gtagcgggat
     5341 tcgcctaata ccgcaacgat atcttccagg ctgtctaaca gacggtgggt tgattcataa
     5401 aaaatcagag tgcgcggctc cgcttcaatg gcttttagcg catcacggcg gccttttgat
     5461 ttggcaggta aaaagccttc gtaacagaaa cggtcagagg gtaaacccgc ggcgcttaac
     5521 gcagtgatag cagcacacgg cccgggtagc ggcaccacgc gaatccccgc ttcacggcag
     5581 gtacgcacca gatggtagcc aggatcgtta attagcggcg ttccggcatc ggaaaccagc
     5641 gcaatgtttt gcccctcttg cagcttcgcc agcagcgttt cggctttttg ttgttcgtta
     5701 tggtcgtgca gcgcaaacaa ccgggcatta atcccaaaat gttgcagcaa taaaccggtg
     5761 tgacgagtat cctcggcggc aatcagatca acggcctgta atacctctaa cgcacgctgg
     5821 gtgatatccg ccagattgcc gattggcgtc ggtacaatgt aaagctggcc ctgagaatta
     5881 tccgccgatt ggtgttgttt cattgtgtcg tccgtattgc cgatttaata ttgagcattg
     5941 cgtaaaaaaa atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc
     6001 gcgcgttgtc tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact
     6061 cccgatcagt ccactgctta tatgcagggc acggcgcagg ctgattctgc cttttatctt
     6121 cagcagatgc agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt
     6181 gcactggtga aagaaggtaa aaccgggcag gcggtggagt tgtttaacca actaccgcaa
     6241 gaactgaacg attctcagcg tcgcgagaaa acactgctgg cggtagagat taaactggcg
     6301 cagaaagatt ttgctggcgc gcaaaatttg ctggcgaaaa tcacacctgc cgatttagaa
     6361 caaaaccagc aagcccgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc
     6421 attgatttac tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa agaaaaacag
     6481 cagaatattg atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg
     6541 ctggtgatca acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg
     6601 tttgataacc gaaacgatcc cgacatgatg aaagccggga tcgccgactg gcagaaacgt
     6661 tatccgaaca atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt
     6721 aaaccagcct cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta
     6781 tttggtcgca ctattcagca aggctttgaa gcggcgaaaa atatcggcac tcagccagtg
     6841 gcggctcagg tagctgccgc acctgccgca gacgtagcag aacaacctca gccgcaaacc
     6901 gtggatggcg ttgccagccc ggcacaagcc tcggttagcg atctgaccgg tgaacagcct
     6961 gcagcccagc cggtgcctgt aagcgccccg gcgacaagca ccgcagcggt aagcgcaccc
     7021 gcaaatccat ccgcagagct gaaaatctac gatacctcat cacaaccact tagccagatc
     7081 ttaagccagg ttcagcagga tggcgcgagt attgtggtcg gtccgttgct gaaaaataac
     7141 gttgaagagt tgctgaagag caacaccccg ctgaacgtgc tggcactgaa ccagccggag
     7201 aatatcgaaa accgcgtcaa tatttgttac ttcgcgcttt cacctgaaga cgaagcgcgc
     7261 gatgcagcgc gtcatattcg tgaccagggt aaacaagcgc cgctggtgct gatcccacgc
     7321 agttcattgg gcgatcgcgt agccaatgcg tttgcgcaag agtggcagaa actgggcggc
     7381 ggcaccgttc tgcaacaaaa atttggttcc accagcgaat tacgcgcggg tgttaacggc
     7441 ggttctggta ttgctttaac gggtagcccg attactccca gagcgacaac cgactccggc
     7501 atgacgacca acaatccaac gctgcaaacc acgccaaccg atgaccagtt caccaataat
     7561 ggcggtcgtg tcgatgcggt gtacattgtg gcaacgccgg gtgaaatcgc ttttatcaaa
     7621 ccgatgatcg ccatgcgtaa cggtagccag agcggtgcaa cgctgtacgc cagctcccgc
     7681 agtgcgcaag ggaccgctgg cccggatttc cgactggaga tggaaggctt gcagtacagc
     7741 gaaatcccga tgctggcagg cggtaatctg ccgttaatgc agcaggcgct cagcgcggtg
     7801 aacaacgatt attcgctggc tcgcatgtat gcgatgggcg ttgatgcctg gtcgttggca
     7861 aatcatttct ctcaaatgcg ccaggttcag ggttttgaaa tcaacggtaa taccggaagc
     7921 ctgacggcaa acccggattg cgtgattaac aggaagttat catggctaca gtaccaacaa
     7981 ggtcaggtag tccccgccag ttaaccacca aacagaccgg cgatgcgtgg gaagcacaag
     8041 cgcgtcgctg gctggaaggc aaaggactgc ggtttatcgc cgctaacgtg aacgagcgtg
     8101 gcggcgagat cgatctgata atgcgtgaag gccggaccac cgtttttgtc gaggtacgct
     8161 atcgccgctc tgcgctttat ggcggcgcgg cagccagtgt gacccgcagc aaacaacaca
     8221 aattattaca gactgcccgc ttgtggctcg cgcgtcataa tgggagtttt gatactgtgg
     8281 attgccggtt cgatgtggta gccttcaccg ggaatgaggt tgagtggatt aaggatgcct
     8341 ttaatgacca ctcataatta aggtttaagg attagcgtgc aagaaagaat taaagcttgc
     8401 ttcactgaaa gcattcaaac tcaaattgcg gcggcagagg cgcttccgga tgccatctcc
     8461 cgtgcagcca tgacgctggt tcagtctctg ctcaatggca acaaaatcct ctgttgtggt
     8521 aatggaactt ccgctgccaa tgcacagcat tttgctgcca gcatgatcaa ccgtttcgaa
     8581 acggagcggc ccagcttacc tgccattgca ctaaatactg ataatgttgt cttaacggcg
     8641 attgccaacg atcgcttaca tgatgaagtg tatgcaaaac aggtgcgggc gctgggtcat
     8701 acgggagatg tattgttagc catttccacg cgtggcaaca gccgcgatat tgtaaaagcg
     8761 gtagaagctg ccgttacgcg tgatatgacc attgtggcat taaccggcta tgacggcggt
     8821 gaacttgcag gtttgttagg gccacaggat gtggaaatcc gcattccttc gcatcgtagt
     8881 gctcgcattc aggaaatgca tatgctgacg gtaaattgcc tgtgcgatct gatcgataac
     8941 acgcttttcc ctcaccagga tgattaagga gaatacatga aggcattatc gccaatcgca
     9001 gtccttattt ccgcgctgct gttgcaaggt tgtgttgccg ctgccgtagt gggtaccgct
     9061 gctgtgggta ccaaagccgc aactgaccca cgcagtgtcg gcacccaggt ggacgatggt
     9121 accctggaag tgcgcgtgaa cagcgcattg tcgaaagacg aacagattaa gaaagaagcg
     9181 cgcattaatg taacggccta tcagggcaaa gtgctgctgg ttgggcagtc accaaacgct
     9241 gaactttcgg ctcgcgccaa acagattgct atgggcgtag acggtgccaa cgaagtgtat
     9301 aacgagattc gtcagggcca accgattggt ctgggcgaag cgtctaacga tacgtggatc
     9361 accaccaaag tgcgttcgca gctcttaacc agcgacctgg tgaaatcgtc caacgtgaaa
     9421 gtgaccaccg aaaacggtga agtgttcctg atggggctgg tgactgaacg tgaagcgaaa
     9481 gcggcggcag atattgccag ccgggtaagc ggcgtgaaac gggtaactac ggcgtttacg
     9541 tttattaaat agcagcccct tgtaatgcct gatgcgacgc ttgccgcgtc ttatcaggcc
     9601 tacaacgaca caaaacgtag gtcagataag gtgtttacgc cgcatccgac tgctgttgcc
     9661 tgatgcgacg cttgccgcgt cttatcaggc ctacaacgat acaaaccgta ggtcagataa
     9721 ggcgtttacg ccgcatccga catgttttcc ctcaaatcag aacaacagcg ccagcccgcc
     9781 gacaatcaca ccagacactg ccaccatcgc ccccgtcagc cataaggctt tcgccgggaa
     9841 cgctttgcgc agcataatca gtgacggcaa actcaccgcc gggagcgtca tcaacaatgc
     9901 cagcgccgga gcagttccca tacctgccag catcatcgtt tgtacaatcg gaatttctgc
     9961 tgccgtggga atgacaaaca agcatcctgc caccgccatc gccaccaccc acatcaggct
    10021 gttatcgaca gtaccatcgg catgggggaa taaccagacg cgagcggcac ccaacaccag
    10081 tactgcaagg atgtaaaccg ggatcgtact ccagaaaagc gtccatagcg ccctgcccca
    10141 gcggctaaaa aaaccgccct gtgcttccgg tatgtcaatt tcgaccggtg cctgcgtttg
    10201 cggtgtttca cgcacccatt tttgcaccag cgtcgcaatc agcaacacca tcaccagccc
    10261 ggccaccaga cgaatcgccg caaaacccca gccgaggaca aagcccataa acaccagcgt
    10321 cgccgggttt aacaccggat tgcccatcca gaatgccagc gcaccgccca tcgacacctg
    10381 ttggcgacgc attcccgccg cgaccggagc cgcacagcag gtacacatca tgcccggcaa
    10441 cgaaaacagc gttcccagca gcgtgccgcg aaagcgcgat tgcccaagcg tacgcaacaa
    10501 ccagtcacgc gggatcagca cctgaatcaa cgaaccgagg atcaccccca gcaccgccgc
    10561 tttccatacc gcgaggaaat agatcatcgc gtaatccaac gccgcctgcc atgggttagc
    10621 atccgcctgc gcaaggatag atttaccgat actgtgggtt tcggcagcag taaacgcttt
    10681 gccgtagtaa ggttcccatt tcacatacca gagaccggca atgacaacga gaaagaaaag
    10741 cgcgggtttc caccactgaa tgggcgttgc cgcctgagat gaagactgac cagtcatagc
    10801 attccccagg aataagttat gataattgag cgcgtgaata ttacgctcac tatcaattct
    10861 tgggaataat tattcagctc ttttgcgtaa ttctgaagag cttaaaatcg tcacgccttc
    10921 atgctcaggc cgcatcgact ctgccagcat gacccgcgcc acgtctctcg catcaatgga
    10981 tttccagtta cctggtaaca aacggaacag cggcgcaaaa agcgtttcgt tcatccgctg
    11041 tttgctacga tcgcccagta acatcgacgg gcgagcaatg gtcaatttcg gccagttctg
    11101 ggcaattaat gcttcttcca tctccccttt gacgcggtta tagaaaaacg gcgagtgggc
    11161 attggcaccc atagcactga ccaccagcat atgctgcgcg cccagccgcc gcccggttaa
    11221 tgcggtatcc actaccagcg tgtaatcggc atgaataaac gcctctttgc tccccgcttc
    11281 tcgccgcgtg gtgccgagac aacaaaacac aatgtcgatg ggatcggtga cctgcgccag
    11341 cgcgtcagtc agttgcggat catggggatt gaacactcct ggcatatctc ccaacggacg
    11401 tcgcgtcggt gcagcaatag cgttaacttt cggttcgtta atcaacatcc gcagcaggtg
    11461 accgcccacc agccccgttg cgcctgtaat cagtacctga ctcatcttcg ctcctttaca
    11521 gaattgtctg ccttgcgctc cccggctcat gcaccaggct taatagacca tgaggtaatt
    11581 atccccgatt gtgagaaatt cgcctcatcc aatgcaacaa cgcggaggaa gcatgagtaa
    11641 gaaaattgcc gttttaatca ctgatgaatt tgaggattca gaatttactt cacccgcaga
    11701 cgagttccgt aaagccggac acgaagtgat taccattgaa aaacaagcgg gtaaaacggt
    11761 gaaaggcaaa aaaggagaag ccagcgtgac catcgataaa tccatcgatg aagtgacgcc
    11821 agcggagttt gatgccctgt tgctaccggg cggtcattca ccggattatc tgcgtggcga
    11881 caaccgtttt gtcaccttta cccgtgattt tgtgaatagc ggcaaacctg ttttcgccat
    11941 ctgtcacggt ccgcagttgc tgatcagcgc cgatgtgatt cgggggcgca aactgaccgc
    12001 agttaaaccg atcattattg atgttaaaaa tgcgggcgcg gaattttacg atcaggaagt
    12061 cgtggttgat aaagatcagc tggttaccag ccggacaccg gacgatctgc cagcgtttaa
    12121 ccgcgaggcg ttacgcctgc tcggtgcctg agtcgcgtag ccaaatcatt tttttaccaa
    12181 agcccagcgt gttgtcggtg aatttgattt catcgagccg gatttcccac accggggccg
    12241 acagcattct ggcaaccgga aagcgacgat tgtacgcctt gcgcgcgagg tcgctttcct
    12301 caccttccag cctgcggatc tcacctttaa actgcacacc gcgaattaac gctaccgttt
    12361 tcggctgacc gtttaccgtt ccggcaactg ccgcctgcgg cccgctcatc tgcgcgtggc
    12421 gcgttttttc ttccgtcaaa atgtagaagg cgactttctg cgcatcaaaa agataaaagg
    12481 cattagcgca ccagagttcc ccttcctgct gcacacacca ggtgacaaca tgttgttttg
    12541 ccagccaacg gctgatggcg atgagtgttt ccattgctgt tctcttttat actgtgggcc
    12601 tgaccttaac atgcgatgca gacgatgaca ccctggtttc tttacttgat ccgtaccgcc
    12661 gacaataagc tttataccgg gatcaccacg gatgtcgaac gccgctatca gcagcaccaa
    12721 agcggcaaag gggcgaaagc actgcgcggg aaaggagaac tgacgctggc gttttctgct
    12781 ccagtgggcg atcgttcgct ggcgttacgg gcggaatatc gcgttaagca actgaccaaa
    12841 cggcaaaaag agcgtctggt agcggaaggc gcagggtttg cagagctgtt aagcagtctg
    12901 caaaccccgg agattaaaag cgattgaaat gctcgtgata ctcaaccagg ccggtaacgc
    12961 cattcagcgc gtcatccgct aaacgatgta cctggaaggc gctttcagtg cccggccagc
    13021 ggcaacgcag atcgtgatgc gccgccagtt caaagccgaa acgactgtac agcgccggat
    13081 cgcccagcgt caccactgcg gcatagccga actcattaag cgaatcgagt ccttcataga
    13141 ccagttggcg tgccagcccc tgtccacggt atttttcatc aaccgccaac ggtgccatgc
    13201 cgacccattg caggtcttcg ccctgcacat caaccggact aaatgccaca tagccaatga
    13261 cctgaccttc gtcatctgtc gccaccagcc ccagcgtcag aaagccatct tcacgcagat
    13321 cgtgaaccag cttcgcttcc gcatcacttt cgaatgagcg acgcagcagg gcatcaatac
    13381 ccggcgcatc aatgggaatt tctactcgaa ttagcatggt tcacctaccg atgtctgttt
    13441 ggtttcaggc gcggttttca ttcccgcctc aacaaaatcc gccagttgca gcagcatcat
    13501 gcgtaaggct ttcggcattt gctccagttc aatggcgtcc atcaggtttt tcacatacag
    13561 ccccagctcc gtatcgcctt caatcaccag ccgacgctgg aagaagagcg tatccggatc
    13621 ttgtttacgc gccgcaatca tcagcagatc gctggcgtcg gcactaaaac tcacatcagc
    13681 ttgcgcgttc tggctgacca ccagtttgcc attcaccacc gaggtaaacc attgcaggtc
    13741 aatatcacgc acatgaatac ttaaccagcg gccttcaaga aactccagct cgccatcatc
    13801 cagcgcctga cggaattgcc agcttaagac ctgctcaaga acctggcgtt ttagcgcaaa
    13861 tggcgtcagt tttaccggta cactcaacag agatggcccc aaatgcacaa tacgggaacg
    13921 cagtttatcc aacacgagtt ttactccctg tttcaacaat catcctattt tgccatatca
    13981 gaaaaataac atagcggtat aaatcaacaa ttccatatga aattgctgct accaccaata
    14041 caactttaac tgccttaaat caaaaattgt cgcagcaagg ttaactaaaa tcccagttcg
    14101 ttaacatttt tgcgttttga tagcgcaacc ttcaggaaaa attatggagc tgctctgccc
    14161 tgccggaaat ctcccggcgc ttaaggcggc catcgaaaac ggcgcagatg ctgtttatat
    14221 cgggctaaaa gatgatacta atgcccgtca cttcgccggc cttaacttta ccgagaaaaa
    14281 attgcaggaa gcggtgagtt ttgtccatca acatcgccgc aaacttcaca tcgcgattaa
    14341 cacttttgcg catccggacg gttacgcccg ttggcagcgc gccgtggata tggcggcgca
    14401 gctgggtgcc gacgcgctga tcctcgccga cctcgccatg ctggagtacg ccgccgagcg
    14461 ttacccgcat attgagcgtc atgtgtcagt gcaggcttcg gcgaccaatg aagaggcgat
    14521 taacttttat catcgccatt ttgacgttgc tcgcgtggtg ctgccgcgcg tgttgtcgat
    14581 tcatcaggtg aaacagctgg cacgggttac tcctgtcccg ctggaagtgt ttgctttcgg
    14641 cagcctgtgc attatgtcgg aaggtcgttg ctatctgtcg tcgtatctga cgggtgagtc
    14701 gcccaacacc gtgggagcgt gttctccggc ccgtttcgtg cgctggcaac aaacgccgca
    14761 ggggctggaa tcccgcctga acgaagtgct gatcgaccgt tatcaggacg gcgaaaacgc
    14821 aggttatccg acgctgtgta aagggcgtta tctggtggac ggcgagcgct atcacgcgct
    14881 ggaagaacca accagtctca ataccctgga actgctgccg gagttaatgg ctgcgaatat
    14941 tgcttcggtg aaaattgaag gccgccagcg tagcccggcg tatgtcagcc aggtggcgaa
    15001 agtctggcgt caggctatcg accgttgtaa ggccgatccg caaaactttg taccgcaaag
    15061 cgcgtggatg gagacgctcg ggtcgatgtc cgaaggcacg caaaccactc ttggcgcata
    15121 tcaccgtaaa tggcagtgag aaaagcaatg aaatattcct tagggccagt gctgtggtac
    15181 tggccaaaag agacgctgga agaattttat cagcaggccg ccaccagcag cgccgacgtg
    15241 atttatcttg gtgaagcggt atgcagcaag cgtcgggcaa ccaaagttgg cgactggctg
    15301 gagatggcaa aatcgctcgc cgggagtggt aagcagattg tgctctccac gctggcgctg
    15361 gtgcaggcat catctgaact gggcgaactg aaacgctatg ttgagaacgg tgagtttctg
    15421 attgaagcca gcgatctcgg cgtggtgaat atgtgcgctg aacgcaaact gccgttcgtc
    15481 gccgggcacg cgctgaactg ctacaacgcg gtgacactga aaatattgct caaacagggc
    15541 atgatgcgct ggtgtatgcc agtggagctt tcccgcgact ggctggtgaa tctgcttaat
    15601 cagtgcgatg agctgggcat tcgtaaccag tttgaagtgg aagtcctgag ctacggtcat
    15661 ctgccgctgg cctactccgc ccgctgcttt accgcgcgtt cggaagaccg cccgaaagat
    15721 gagtgtgaaa cctgctgcat taagtatccg aacgggcgca acgtgctgtc gcaggaaaac
    15781 caacaagtgt ttgtactcaa tggcattcag accatgagcg gctacgttta caacctcggt
    15841 aacgagctgg catccatgca gggcctggtt gatgtggtcc gcctgtcacc gcagggtact
    15901 gacactttcg cgatgctcga cgccttccgc gctaatgaaa atggcgctgc accactgccg
    15961 ttgacggcga acagcgactg taacggctac tggcggcggc tggccgggct ggagctgcaa
    16021 gcctaagtaa atatgctcgt catacttcaa gttgcatgtg ctgcatctgc attcgttcac
    16081 cccagtcact tacttatgta agctcctggg gattcactct cttgtcgcct tcctgcaact
    16141 cgaattattt agagcacagc tcactttgtt aacaacttta actactcttt aatgcagtat
    16201 taaagattaa tcggtaacaa agtgagctgt tatgactgat aaaaccattg cgttttcgct
    16261 actcgatctg gcccccattc ccgaaggttc ttcagcgcga gaagcattct cccactctct
    16321 cgatctcgcc cgtctggctg aaaagcgcgg ctatcatcgc tactggctgg cggaacacca
    16381 caatatgacc ggcattgcca gtgctgcaac atcggtattg attggctatc tggcggcgaa
    16441 taccaccacg ctgcatctgg ggtctggcgg cgtgatgttg cctaaccact caccgttggt
    16501 cattgccgaa cagttcggca cgctcaatac gctctatccg gggcgaatcg atttggggct
    16561 gggtcgtgct ccgggtagcg atcagcgaac catgatggcg ctacgtcgtc atatgagcgg
    16621 cgatattgat aatttccccc gcgatgtcgc ggagctggtg gactggtttg acgcccgcga
    16681 tcccaatccg aatgtgcgcc cggtaccagg ctatggcgaa aaaatccccg tgtggttgtt
    16741 aggctccagc ctttacagcg cgcaactggc ggcgcagctt ggtctgccgt ttgcgtttgc
    16801 ctcacacttc gcgccggata tgttgttcca ggcgctgcat ctttatcgca gcaacttcaa
    16861 accgtcggct cgactggaaa aaccatacgc gatggtgtgc atcaatatta tcgccgccga
    16921 tagcaaccgc gacgctgagt ttctgtttac ctcaatgcag caagcctttg tgaagttgcg
    16981 ccgtggcgaa accgggcaac tgccgccacc gattcaaaat atggatcagt tctggtcgcc
    17041 gtccgaacag tatggtgtgc agcaggcgct gagtatgtcg ttggtaggtg ataaagcgaa
    17101 agtgcgtcat ggcttgcagt cgatcctgcg cgaaaccgac gccgatgaga ttatggtcaa
    17161 cgggcagatt ttcgaccacc aggcgcggct gcattcgttt gagctggcga tggatgttaa
    17221 ggaagagttg ttgggatagt gtgtcttaac gcgggaagcc ttatccgagc tggcaacgct
    17281 gtcctacgta gacctgataa gcgaagcgca tcaggcattg tgtaggcagc agaaatgtcg
    17341 gataaggcac cgctgattac tgatacaccg gcagtaaatt aaagctcgat aaaatatgga
    17401 ccagtgcgtt gccaacgcca aacaccagaa tcagcgtaat catcggcttg ccgccccaga
    17461 cgcggaattt cgggctgcca aagcgtttac gcgatgcgcg ggctaacagc gccggaacaa
    17521 ttgccgccca aatggtagct gctaaaccag cataaccaat ggcgtacagg aatccgttcg
    17581 ggaacagtag accacccaca acaggcgggg caaaggtcag caatgccgtt ttcaagcgcc
    17641 ccagggctga gtcatcgaaa ccaaacagat ctgccagata gtcaaacaaa cccagcgtta
    17701 cgccaaggaa cgaactcgct accgcaaagt ttgagaacac gaccagcaga aggtccaggc
    17761 tacggctgtt cagcacgccg cttaatgcct gtaccagcac atcaatatta ccgcccttct
    17821 ctgcaatacc gataaactcc ggacgcggga tgttacccat cgtcgccagc aaccagatgg
    17881 tatacagcgc cagtgccatc agcgtaccgt acaccaggca tttcacgatg gttttcggat
    17941 ctttgccgta atacttcatc aggcttggca cgttaccgtg ataaccaaac gacgccaggc
    18001 agaacggcaa tgtcatcagc agatacggcg cataagatgc attgctttct gcaacgttga
    18061 acaatgtcgc aggctgcaca tgccccaaca ggctgccaaa ggtgaggaag aaggtaatga
    18121 ctttcgcccc cagcacaatc gccgtcatgc ggctgacggc cttagtactc aaccacacca
    18181 caaacgctac cagcagtgca aaaccaaaac ccgccgcccg tgccgggacg tttagcgaca
    18241 tctctgcgaa ggtgtgatgc agaatcgaac cactggcaga aatataggca taggtcagga
    18301 tatagagcac aaaggcaatg gaaataccgt tgaccacgtt ccagcctttg cccagcaaat
    18361 ctttggtgat ggtgtcaaaa ctcgaaccga ttcggtaatt caggttagct tccagaatca
    18421 tcaagccgga atgcagcata cagaaccagg taaagatcag cgccgccatt gaccagaaaa
    18481 accacgcccc ggacatgacc actggcagag aaaacatccc tgcgccaata atggtgccgc
    18541 cgataatcac cacgccgcca agcagcgacg gtgacgtttg ggtggtggtt agtgttgcca
    18601 tgagggcttc tctccagtga aaaatagtgc gactgcgttg ttatgcattg cactgtacca
    18661 gtacacgagt acaaaagaca gaaaaaaagc cccgatggta aaaatcgggg ctgtatatat
    18721 tattttacag attgtgttcg ctgttcagcg atgattacgc atcaccaccg aaacgacgac
    18781 gaccggtaga gtcatcacga cgcggagcgc ggccttcacg acgttcgccg ctaaaacgac
    18841 gaccatcacc acggccacct tcacggcgtt caccgctgaa gttacgaccg ccttcacgac
    18901 gttcgccacc gaaaccacga ccaccgccac gacgctcacc gccagtatgc ggctgtgcat
    18961 cgcccagtaa ctgcatgttc atcggcttgt tgagaatgcg agtgcgcgta aagtgttgca
    19021 gcacttcacc cggcatacct ttcggcagtt cgatggtgga gtgagaagca aacagcttga
    19081 tgttaccaat gtaacggctg ctgatgtcgc cttcgttagc aatcgcacca acgatatgac
    19141 gaacttcaac accatcatcg cggcccactt caatgcggta cagctgcata tcgccaacat
    19201 cacgacgttc acgacgcgga cgatcttcac ggtcaccacg cgggccacgg tcgttacgat
    19261 cgcgcggacc acggtcatca cggtcacgga attcacgttt cggacgcatc ggcgcatctg
    19321 gcggtacgat cagagtacgt tcaccctgtg ccattttcag cagtgccgca gccagagttt
    19381 cgagatccag ctcttcacct tctgcagtcg gctgaatttt gctcagcagt gcgcggtatt
    19441 gatccagatc gctgctttcc agctgctgct gtactttagc ggcgaatttt tccagacggc
    19501 gtttgcccag cagttctgcg ttcggcagtt ctacttccgg aatagtcagc ttcatagtac
    19561 gttcaatgtt gcgcagcaga cgacgctcgc ggttctcaac gaacagcagc gcgcggccag
    19621 cacgacccgc acgaccggta cgaccgatac ggtgaacgta agactcagaa tccatcggga
    19681 tatcgtagtt aactaccagg ctgatacgct caacgtccag gccacgggct gcaacgtcgg
    19741 tcgcaatcag gatgtccaga cgaccatctt tcaggcgttc cagtgtctgt tcacgaagcg
    19801 cctggttcat gtcaccgttc agcgcggcgc tgttgtagcc gttacgctca agagcttcag
    19861 ccacttccag agtcgcgttt ttggtacgaa cgaagataat cgccgcatca aaatcttccg
    19921 cttccaggaa acgtaccagt gcttcgtttt tgcgcatacc ccagacagtc cagtagctct
    19981 ggctgatgtc aggacgggta gtcacgctgg actgaatgcg cacttcctgc ggctctttca
    20041 taaagcggcg ggtaatgcga cgaatcgctt ccggcatggt tgcagagaac agagcggtct
    20101 gatgaccttc cgggatctgc gccataatgg tttcaacgtc ttcgatgaag cccatgcgca
    20161 gcatttcgtc agcttcatcc agaaccagac cgctcagttt agagaggtcc agagtgccac
    20221 gtttcaggtg gtccagcaga cggcccggag taccgacaac gatctgcggc ccctgacgca
    20281 gggcgcgtaa ttgcacgtca taacgctggc cgccgtacag agcaaccaca tttacgccgc
    20341 gcatgtgttt agagaaatcc gtcattgctt cagcaacctg taccgccagt tcgcgggtcg
    20401 gtgccagcac cagaatctgt ggtgctttca gctcaggatc aagattctgc aacagaggta
    20461 aagagaatgc tgcagttttt ccgctccccg tctgggccat acccagaacg tcgcggccat
    20521 tcagcagatg tggaatacac tctgcctgaa ttggagatgg tttttcgtaa cccagatcgt
    20581 taagggcttc aaggatagga gccttcaggc ccagatctgc aaaagtggtt tcgaattcag
    20641 ccatgtagta cgtgtgcctc aaaattaatg gcggccagtc tacataactc atcatgaaat
    20701 tgatcagcaa ttttcattga aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa
    20761 caacgccctc acccgttaag gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt
    20821 cagctattgc tggtccgatt ctgccaggtc atcttggtcc tggcccagga gcgataattc
    20881 caacaatgcg tatcggtgct caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa
    20941 cagtgccgtg gcgctgtcca aatcccccag acttaggtag tacttaccta aatagaagtt
    21001 ggtttcactg agatgctcag cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc
    21061 cattaacgtt tgttcgctaa tgttgcccag gtagaactcg acaatgttcc atccccactg
    21121 ttccttatcc gatttttcga agtgctgttt caacacttct ttagcctgct tctcatcgag
    21181 cttctgctcg gcgagataaa gccacagact acggaaagga tcattgggat cgtcttgata
    21241 aaacgccagc agatcatctt gcgctaactt gtcacgaccg ccgtaatata atgcgatccc
    21301 gcgattcaag tgcgcgtagt tgtaagttgg atcaagctca agtacagaat caaacgcttc
    21361 ataggcagca tcaaaattgc ctgcctgcgt taaatatatg cctaagtaat tgaatacttc
    21421 aggcatatcc ggtcggattg ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag
    21481 accgagacta tcatacaaca ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt
    21541 taaagcccga ctggcaagga tttgttccat acgtgccaga atcacttcct gctgtaaagt
    21601 cggttgcaat ggtaccgcga ggacttcact tttacgccag gaagtattac tgcatcctgc
    21661 aagcgtaagt gctgtcgcaa cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg
    21721 aagaccacgg ttgaatgaac gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta
    21781 aaagcccccc gccgcagcgg agggcaaatg gcaaccttac tcgccctgtt cagcagccgg
    21841 agcttccggt gctgcagcag gttgagactg ctcagtcgct tctttaatgc tcagacggat
    21901 acggccctgg cgatcaactt ccagaacttt caccggtact tcctgaccca tctgcaggta
    21961 atcggtcact ttctcaacgc gtttgtcagc gatttgagag atgtggacca gaccttcttt
    22021 accgccgccg atggcaacaa atgcgccaaa gtcaacgata cgggtcactt taccagtgta
    22081 gacgcggccc acttcgattt ctgcagtgat ctcttcgata cgacgaatag catgtttcgc
    22141 tttctcgccg tcggtcgctg cgatcttcac agtaccgtca tcttcgattt cgatggtagt
    22201 gccagtttct tcggtcaggg cacggataac agagccgcct ttaccgataa cgtctttgat
    22261 cttatccggg ttgatcttga tggtatggat acgcggtgcg aactcagaga tatcgccacg
    22321 cggcgcgttg atcgcctgtt ccattacgcc caggatatgc agacgcgcac ctttagcctg
    22381 gttcagcgca acctgcatga tctctttggt gataccttca attttgatat ccatctgcag
    22441 tgcagagata ccgtcgcggg aacctgcaac tttgaagtcc atatcgccca ggtgatcttc
    22501 gtcgcccaaa atgtcagaca gtacaacgta gttgtcgcct tctttcacca gacccattgc
    22561 gatacccgca acggcagctt tgattggcac acctgcgtcc atcagcgcca gagacgcgcc
    22621 gcacacggaa gccatagaag aagaaccgtt ggattcggtg atttcagaca ctacacgtac
    22681 ggtgtacggg aatttgtcca tatccggcat gactgccagc acgccgcgct tcgccagacg
    22741 accgtgacca atttcacgac gcttcggaga accgaccatg ccggtttcgc ctacggagta
    22801 cggagggaag ttgtagtgga acaggaaggt atcggtacgt tcgcccatca gttcatcaag
    22861 aacctgcgcg tcacgagcgg tacccagcgt tgcagtaacc agtgcctgcg tttcaccgcg
    22921 ggtgaacagc gcagaaccgt gagtacgcgg cagcacgcca gtacgcacat ccagaccacg
    22981 gatcatatct ttttcacgac cgtcgatacg cggttcgcct gccagtacgc ggctacgaac
    23041 aacgtttttc tcgatagcgt gcagaatttc acccagttcg ttttcgtcca gggtttcgtc
    23101 ttcagcaagc agcgtcgcga tggtttcaga tttgatgaca tcaacctgcg cataacgctc
    23161 ttgtttgtcg gtgatgcggt aagcatcgct caggcgagct tcagccagtg cagcaacgcg
    23221 cgcgttcagc gcttcgttta ccggctccgg ctgccagtcc caacgtggtt taccggcttc
    23281 tttcaccagt tcattgatgt tctgaataac aacctgctgt tgttcatgac cgaacactac
    23341 tgcgcccagc atctggtctt cgctcagcag ttcagcttca gattcaacca tcagaacagc
    23401 ggcttcagta ccggcaacaa ccagatccag tttgctctct ttcagctcgt cctgagtcgg
    23461 gttcagtacg tactggtcat tgatgtaacc tacgcgagca gcaccaatcg gaccattgaa
    23521 cggaatacca gacagagaca gtgctgcgga agcaccaatc atcgcgacga tatccgggtt
    23581 aacttgcggg ttaacagaaa ccacggtggc gataacctga acttcgttga cgaagccttc
    23641 cgggaacagc gggcgaatcg ggcggtcaat cagacgcgcg atcagggttt cgccttcgct
    23701 tgggcggcct tcacgacgga agaagctacc cgggatacga ccagcagcgt aggtacgctc
    23761 ctgatagtta acggtcagtg ggaagaagtc ctgacctggt ttggcttttt tctggccaac
    23821 aacggtaaca aataccgcgg tgtcatccat gctaaccata acagcggcag tagcctgacg
    23881 agccatcatg ccggtttcca gagtcacggt gtgttggccg tactggaatt tacgaacgat
    23941 cggattaagc aatgtaatat cctttctctt tcttagacag taccttacgg cactggtgtt
    24001 aatacccgat cttctgcgca tcctcgcgac taatgacaac cctaacccag ctctatgtgg
    24061 gtaaagcctc tcattagccg cgcgaacctc tgcaacggaa gatcattcat agcaacaata
    24121 cattagtttc cagtgaattg ctgccgtcag cttgaaaaaa ggggccactc aggccccctt
    24181 ttctgaaact cgcaagaatt agcgacgcag acccaggcgc tcgatgagct gggtgtaacg
    24241 tgctacgtct ttacgtttca ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag
    24301 cagaccacga cggctgtggt gatctttttt gtgctctgca aagtggccct gcaggtggtt
    24361 gatctgtgca gtcagcagtg ctacctgaac ttcggtagaa ccggtgtcgt ttgcgtcacg
    24421 accaaactca gaaacgattt tagctgttgc ttcagtactt agagacattt taaaactcca
    24481 aagtatatag aatgaaagga cgccgatctc taattcagcg atcccagtgt acgttacgca
    24541 aagtgttaaa caatttacgc gacgttaagc ggcagtattc tactcgtagc gacctgttat
    24601 cgcaagacgg ttaacattac gccgggtatt caaccaccag gcgacgaggc gcaacgcggc
    24661 cttcatcgtc aatttcgccc ataccgataa atttgccgtt ctcaccttct gtaacgcgaa
    24721 ccagtccttc cagcggcgca ccggatgtac gaactgggtt accatttttg aagtaaacag
    24781 aagacgttaa cggaagattc accaccgggt agtccgaagc tggactgtcc attggcatca
    24841 gtaatggatc aagtaactcc gcagccggaa tatcctgctg ttcggcttgt tcgaccagtt
    24901 cacgcagatg ttccagggtc accatccgtt caaccggata tttacttacc gccagacgac
    24961 gcaggtaaat aacatgcgcg ccacaaccga gtttttcacc caggtcatca atgatggtgc
    25021 ggatataagt gccttttgag cagtgaattt ccagctccag ctcattgcct tcgtggcgaa
    25081 taaacagcaa ttcataaacg gtaatcgggc gcgcttcacg cggtacttca atgccctgac
    25141 gcgcatattc gtacagtttt ttaccctgat atttcagtgc tgaatacatc gaagggatct
    25201 gttcgatatc gccacggaaa gtatccagcg ccgccgccag ttgctctgcg ctaaaggtta
    25261 ccggacgttc ttcaacgatt tgtccgtcgg catcagaagt atcggtacgc tgtccaagac
    25321 gcgcaatgac ccgatagcgt ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt
    25381 ccccgaggca aattggcaac atgccagttg ccagcgggtc cagcgcaccg gtatgcccgg
    25441 cacggttggc gttataaatc cgtttcactt tttgcagcgc atcattgctg gacataccct
    25501 gcggtttatc cagcaacaaa acgccgttaa tgtcgcgacc gcgacgacga ggacgactca
    25561 ttagtcctcc ttgctgtcgt ccgggttaac acgacgttct tcgtcatgtt tgaccacgct
    25621 ggtcaccagg tttgacatgc gcatcccttc aaccagagag ttgtcgtaga agaaggtcag
    25681 ttccggcacg atacgcaggc gcatcgcttt ccccagcagg ctgcggatga aaccagaagc
    25741 ttcttgcaac gctttgatgc ccgctttaac cgcgtcttca tctttgtcgt tgaggaacgt
    25801 cacatatact ttggcatacg ccaggtcgcg agacatttcg acaccggaaa cggtggtcat
    25861 catgcccagg cgaggatctt taatttcacg ctgcaggatg agagcgatct ctttttgcat
    25921 ttcctgcgct acgcgctgcg ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa
    25981 aaaaggggct gttagcccct ttttaaaatt aatttcaggt ggaagggctg ttcacgttgg
    26041 cctgataaga cgcgccagcg tcgcatcagg caatccatgc aggatgtggc gtaaacgcct
    26101 tatcccgcat ggaaccctaa aaaccttaag cgatggtacg ttggatttcg atgatttcga
    26161 atacttcgat cacatcgcca gtgcggacgt cgttgtagtt cttaacgccg ataccacatt
    26221 ccataccgtt acggacttcg ttaacgtcat ctttgaagcg gcgcagggac tccagctcgc
    26281 cttcgtagat aaccacgttg tcacgcagaa cgcggatcgg gttgtgacgt ttaaccacac
    26341 cttcggtaac catacagcct gcgatggcac caaatttcgg tgatttgaac acgtcacgaa
    26401 cttccgccag accgataatc tgctgtttca gttccggaga cagcataccg ctcatcgccg
    26461 ctttcacttc gtcaatcagg ttatagatga cggagtagta acgcagatcc aggctttccg
    26521 cttcaatcac tttacgtgca gaggcatcag cacgtacgtt gaagccaacc aggatggcgt
    26581 tggacgccgc agccagggtg gcgtcggttt cggtgatacc acctacgcca gaaccgatga
    26641 tcttcacttt aacttcgtca gtagacagtt tcagcaagga gtcggagatc gcttccacag
    26701 aaccctgtac gtctgccttc aggacgatgt tcacttcgtg aacttcgcct tcggtcatgt
    26761 tggcgaacat gttctcgagt ttagatttct gctgacgcgc cagtttaact tcgcggaatt
    26821 taccctgacg atacagtgca acttcacgcg ctttcttctc gtcacgtaca acggtaactt
    26881 catcacccgc agccggtacg ccggacaggc cgaggatttc caccggaatg gacggacccg
    26941 cttccagcac ttcctgaccc agttcgttac gcatcgcacg aacacgaccg tattcgaagc
    27001 cacacagaac gatatcgccc ttgtgcagag taccttcacg taccagaacg gtagcaaccg
    27061 gaccacgacc tttatcgagg aaggattcga taaccgcacc gctcgccata cctttacgta
    27121 ccgctttcag ctccagaact tccgcctgca gcaggatagc gtccagcagt tcatcgatac
    27181 cagtacccgc tttcgcagac acatgtacga actggctttc accgccccac tcttccggca
    27241 ggatgccgta ctgggagagt tcgttcttaa cgcgatccgg atcggcttct ggtttatcga
    27301 tcttgttcac tgcaacgacc accggaacct gcgccgcttt cgcgtgctgg attgcttcga
    27361 tcgtctgcgg cattacgccg tcgtcggcag caacaaccag gactacgatg tccgttgcct
    27421 gcgcaccacg agcacgcatt gaagtaaacg cggcgtgccc tggggtgtcc aggaaggtga
    27481 tcatgccgtt ttcagtttca acgtggtatg caccaatgtg ctgggtaatg ccgcccgctt
    27541 cgccagaggc cactttcgtt gaacgaatgt agtccagcag agaggtctta ccgtggtcaa
    27601 cgtgacccat gatggtcaca accggcgcgc gcggttcagc cgcagcaccc gtgtcacggt
    27661 cgctcattac cgcctcttcc agctcgtttt cacgacgcag gataacttta tggcccatct
    27721 cttcagcaac cagctgtgcg gtttcctgat cgataacctg gttgatggtt gccattgcgc
    27781 ccagtttcat catcgctttg atgacctgag agcctttaac cgccatcttg ttcgccagtt
    27841 cgccaacggt gatagtttcg ccgatcacaa cgtcacggtt aacggcctga gcaggcttct
    27901 ggaagccttg ctgcagggaa gaacctttac gttttccgcc tttaccgcca cgtactgctg
    27961 cgcgtgcttc ttcacgatca gcttttgatt cagcgtgttt gttacctttc ttcggacgcg
    28021 ctgctttcgc gttacgacca cggccacggc cgccttcgac ttcacgatcg ctttcgtctt
    28081 cggcctggcg agcatgttga gaagtagtga cgtgataatc gctggaatct tcagtcggtt
    28141 ccgcgttatc agtccatttg ttttcttccg ccatacgacg tgcttcttca gcaacgcgac
    28201 gtgcttcttc ttcgagttta cgacgcgctt cttcttcagc tttacgcttg agctctgcag
    28261 cttcctgctc acggcgggct ttttcagcct gggcgttttt agtcatatcg tcttgttgat
    28321 tgctcacttt gtctttttcc gcagctttac gtttcgcttg ttcagcagct tcacgcttag
    28381 cttgttctgc ggcctcacgt tcagcttttt gttgcgcctc gcgtttagcc gattcttctg
    28441 cctcacgacg ggcttgctct tccgcttcac gctgcgcttg ctcttccgct gcaaggcgtt
    28501 cagcctcttg cggatcgcgt ttcacaaagg tgcgtttctt gcggacttcg atttgtaccg
    28561 atttgctttt tccaccggta ccaggaatgt taagggtgct gcgtgtttta cgctgcagcg
    28621 tcaatttgtc cgggcctgaa tttttctgat tcaggtggtc aatcaaagtc tgtttctctt
    28681 gtgcagacac agagtcgtca gcagacttcc ggatacctgc atcagcaaat tgctgtacca
    28741 ggcgttccac ggaggtctgt cgctctgcgg ccagcgtttt aatcgttaca tctgtcatgc
    28801 tgttccttcc tgctacagtt tattacgctt cgtcaccgaa ccagcaaata ttacgggcag
    28861 ccataatcag tgctccggct ttttcgtcgg tcaacccttc gatatcagcc agatcatcaa
    28921 tgccctgttc ggcgagatct tccagcgtac aaacgccacg ggcggccagt ttgaatgcca
    28981 aatcacgatc taccccttca aggttcagca gatcgtcagc cggtttgtta tcaccgaggc
    29041 tttcttcctg ggcctgtgca atggtggcca gtgcattttt agcacgctcg cgcagtgctt
    29101 caacggtcgg ctcatcaagg ccttcgattt ccaacagctc tttcatcggc acataggcca
    29161 gttcttccag cgtcgagaag ccttcttcta ccagaacagt cgcgaagtct tcgtcgatgt
    29221 cgagatattt ggtgaaggtg tcgatcgctg cgtgcgcttc cgcctgatgc ttagcctgca
    29281 ggtcgtcaac ggtcatcacg ttgagttccc aaccgctcag ctgcgaagcc agacgcacgt
    29341 tctgaccgtt acggccaatc gcctgcgcca ggttaccggc ttcaacggcg atatccatgg
    29401 tgtgtttatc ttcatccacc acgatagaag caacgtctgc cggtgccatt gcgttaatca
    29461 caaactgcgc cgggttatca tcccacagga cgatatcgat acgctcgccg cccagttcag
    29521 tagaaaccgc ctgaacacgc gcgccacgca tacctacaca ggcacctacc ggatcgatac
    29581 gtttatcgtt ggttttcacc gcgattttcg cacgagaacc cggatcgcga gccgctgctt
    29641 taatttcaat cacttcttcg ccgatttctg gcacttcaat acggaacagt tcgatcagca
    29701 tttccggctt ggaacgagtg acgaacagtt gcgcgccacg cgcttccggg cgaacggaat
    29761 agagcacgcc acgaacgcgg tcgccagggc ggaagttttc acgcggcagc atatcttcgc
    29821 gcaggatcac ggcttcagca ttgttgccca gatccagaga gatgttgtcg cggtttactt
    29881 ttttcaccac gccggtgatg atttcacctt cgtgttcacg gaactgatca accaccatcg
    29941 cacgttcggc ttcacgcact ttctgcacga taacctgttt tgccgtctgg gtagtgatac
    30001 ggtcaaaggt aacagactca atctgatctt caacgtaatc gcccaggttc aggctttcat
    30061 cttcataacg tgcggcttca agggtgattt ccttggtcgg ctgggtgact tcatcaacaa
    30121 ctaaccagcg acggaaggtg tcaaaatcac cgcttttgcg atcgatctgt acgcggacgt
    30181 cgatctcttg ttcatatttt ttctttgttg ctgtcgccag cgcgctttcc aatgcttcga
    30241 aaatcttctc gcgaggtagc gccttttcat tggatacggc ttcaactaca gccaaaattt
    30301 ctttgttcat cgcgggcttt tcacctcatc cagactatta aaagtgggga accaggttcg
    30361 ccttctggat attactcagc gcgaacactt catctttacc ttcgacggta actgtgatca
    30421 tttcaccgtc taccgctttg ataacgccct gccatttacg acggttttgt accgccatac
    30481 ggagaaccag agtcacctct tctccgacaa aacgggcgta gtgttcagcc gtgaacagtg
    30541 ggcgatcgag acccggtgag gagacttcca ggttataagc aacggtgatg ggatcttcaa
    30601 catccagcac agcacttacc tggtggctca catcagcaca atcatcaaca ttgatgccat
    30661 cttcactatc aatatagatg cgcagtgtgg atgtgcgacc gcgaataaat tcgatgccaa
    30721 ccagttcaaa acccagggcc tcaactggcg cagtaatcat ctctgttaat ttttgctcta
    30781 atgtggacaa gcccaccccc aagacataaa aaaagggcct aaagcccagt tattctgtag
    30841 tcagataaca aaaaaccccg ataaatcggg gctttatata actgaaccct ataaccgcaa
    30901 ctgcggtctg gagcactttc cagaaggatt ttttcaaatc ccactacgaa ggccgaagtc
    30961 ttcacagtat atttgaaaaa ggactctaag ggaaagtggt tgcgggggcc ggatttgaac
    31021 cgacgacctt cgggttatga gcccgacgag ctaccaggct gctccacccc gcgcctgaaa
    31081 cgtggcaaat tctactcgtt ttgggtaaaa aatgcaaata ctgctgggat ttggtgtacc
    31141 gagacgggac gtaaaatctg caggcattat agtgatccat gccacatttt gtcaacgttt
    31201 attgctaatc acgtgaatga atatccagtt cactttcatt tgttgaatac ttttgccttc
    31261 ccctgctctc ccttaagcgc attattttac aaaaaacaca ctaaactctt cctgtctccg
    31321 ataaaagatg attaaatgaa aactcattta ttttgcataa aaattcagtg agagcggaaa
    31381 tccaggctca tcatcagtta attaagcagg gtgttatttt atgacgacga ttctcaagca
    31441 tctcccggta ggtcaacgta ttggtatcgc tttttccggc ggtctggaca ccagtgccgc
    31501 actgctgtgg atgcgacaaa agggagcggt tccttatgca tatactgcaa acctgggcca
    31561 gccagacgaa gaggattatg atgcgatccc tcgtcgtgcc atggaatacg gcgcggagaa
    31621 cgcacgtctg atcgactgcc gcaaacaact ggtggccgaa ggtattgccg ctattcagtg
    31681 tggcgcattt cataacacca ccggcggcct gacctatttc aacacgacgc cgctgggccg
    31741 cgccgtgact ggtaccatgc tggttgctgc gatgaaagaa gatggcgtga atatctgggg
    31801 tgacggtagc acctataaag gaaacgatat cgaacgtttc tatcgttatg gtctgctgac
    31861 caatgctgaa ctgcagattt acaaaccgtg gcttgatact gactttattg atgaactggg
    31921 cggccgtcat gagatgtctg aatttatgat tgcctgcggt ttcgactaca aaatgtctgt
    31981 cgaaaaagcc tactccacag actccaacat gcttggtgca acgcatgaag cgaaggatct
    32041 ggaatacctc aactccagcg tcaaaatcgt caacccaatt atgggcgtga aattctggga
    32101 tgagagcgtg aagatcccgg cagaagaagt cacagtacgc tttgagcaag gtcatccggt
    32161 agcgctgaac ggtaaaacct ttagcgatga cgtagaaatg atgctggaag ctaaccgcat
    32221 cggcggtcgt cacggcctgg gcatgagcga ccagattgaa aaccgtatca tcgaagcgaa
    32281 aagccgtggt atttacgaag ctccggggat ggcgctgctg cacattgcgt atgaacgtct
    32341 gttgaccggt attcacaatg aagacaccat tgagcagtat cacgcacatg gtcgtcagtt
    32401 gggccgtctg ctgtaccagg ggcgttggtt tgactcccag gcgctgatgc tgcgtgactc
    32461 tctgcaacgc tgggttgcca gccagatcac tggtgaagtt accctggagc tgcgccgcgg
    32521 gaacgattat tcaatcctga ataccgtctc agagaacctg acctacaagc cagaacgtct
    32581 gacgatggaa aaaggcgact cggtgttctc gccagatgat cgtattggtc aattgaccat
    32641 gcgcaacctg gatatcaccg atacccgcga gaaacttttc ggttatgcca aaactggcct
    32701 gctctcctcc tctgccactt caggcgtgcc gcaggtggag aatctggaaa acaaaggcca
    32761 gtaattcgac atcaaccctg cccttcaggg gtggggttac cttgcttatc ctctgtaaac
    32821 tcctttctga gcatcacata atcgaaaaca tcatggtttg catccacaac ccgtgagtct
    32881 cctttatagt gtgcaatcac ttcctcaagc gtttgcggct gttccggctt tgttaccccc
    32941 atccacgcat taataaggta attattgtaa gctgtcgaaa agatgttgtt ttccgttgtg
    33001 cgatccacgc catccccaag aacggggcta taccagataa acatcgggac atgatatgcc
    33061 tgctggctag cctccctgcc tccatgaaaa tagacgttct tcttcgtagg gtcacgttcc
    33121 aggccgtggt cggcaaaata catgaccgag gcgcggcgat cttttaataa ttcaaaaacc
    33181 tgacctagca aactatctgt gtaatgaatg gagttgtcat agcaggcatc ctgatcgtcc
    33241 tgcggttgaa acacggcgct ggattgcgga taggcgctac aagccggttc atggcttcca
    33301 tttaaatgaa gaacaatcag tttcttttgc tgcgtatttt gctgtaatgc ttgcgataaa
    33361 tgcggcaaca acaattcatc aaatcctctg acataaactg tttccatggc gcgcatggcg
    33421 atactggtaa ctgctgtacc attctgccga aaagcggatt gcgagcttag ccagaaagtc
    33481 tgaaatcctg cttgattagc catattaata atgttgtcgg ggtaattatg aatgtcatga
    33541 ctcaaaacag aatcagcagt taaagaaagg ggaaccgaca gcgcagtgta aggtgcgccg
    33601 cttattgctt gattaaacag tttgatctgt tttctttgtg cttcaacttg cggtgtcgta
    33661 gagcgtgtat atccatacaa agacatattg tcgacacgta cagactcccc cacaatcaac
    33721 acgtaggtat caatacctgt atccctgact gataattgaa aatacggcac cgtgtttgca
    33781 attgagagta atctttgatg ctctttcgct gctaaagcaa aatagttgag attgaaaaat
    33841 ggcgtatagg tagcaaatcg cgacgctagt atatatggac tgaacgcttt tttatttttt
    33901 gcatctttat aagcaaattg acaagcggaa aataaactgc ccgaaatgac aatcagcaat
    33961 aatattcctg tcactttttt tgtcggcaag gaaacatcat attttattat tactgccaaa
    34021 aaaagaaggg ataaaaaagc aaaggcacat agataaggga tatacatccc cagcattttg
    34081 acaacttcat ccggatcact ctggagcaca ctaatcgcaa atccatcatt gaaagttgta
    34141 ccaaaggtac accatgaata gaggctaata ctcatgtctg ccgcagtcag taacacaaat
    34201 ggtgcggcaa caagcaggcg aagcggaagg gatgatatac gttttacaac caggatggtc
    34261 aggacgaaga acagcaatcg ctgaaccata tgcgcgccgg aagaggcgac taagttcgta
    34321 ataccaaaaa gaataacaca aagatacaac aaccaatgag atttaaaact tctagcaaat
    34381 ttgttgaata ctgtcatcca gaaatccaag ttcacaacca atataaacaa ttatcaacaa
    34441 tgttcaattt tatatcagac atgtttttca actctgatcc atatcagacc gaaacactgt
    34501 tagttttttc tttttaaatg atgcggccat tgcgttctca gtaattaaat aacattatca
    34561 atgcgtctct aaacattacg cagaacgtag ccatcagtag ttatactgac aggagcagga
    34621 tggtttatcg agaagtaaac atatccgtat tcgtaccgga actggagttg acacaataaa
    34681 gtgccaatta tgtcagcaga agggaaaagt ttataactaa ggcgtaccgg caccatcgtt
    34741 tcaaggtacc agctacgagt aaagcaactg gacgagatac agatacctga caaccattcc
    34801 tcagaccagg accaaaacga aaaaagacgc ttttcagcgt ctcttttctg gaatttggta
    34861 ccgaggacgg gacttgaacc cgtaagccct attgggcact accacctcaa ggtagcgtgt
    34921 ctaccaattc caccacctcg gcacggatac tactttttag ttcgggatat cgctggtcgg
    34981 cttagccgga gcagctggtt gagtttgttc ggttttcacc ggtgcactca gattttccca
    35041 ttcgctacct ttattggttt tgttgctatt gatgttaccc agcaccagac tgatgatgaa
    35101 gaataacgtt gccagcagcg ccgtcatgcg ggtcatgaag ttaccagaac cacttgaacc
    35161 aaacagcgta gcggaagcgc ctgctccgaa ggaggctccc atatcagcgc ctttaccttg
    35221 ctgcagcatg atcagaccaa caaggccaat tgccacaata aggaaaacta ctaaaagagc
    35281 ttcgtacata atcaacctgt tccttgcgga gttgccgcgt accaatgctt caaccaataa
    35341 agcgggagtt tttaatcttt cccactgaag cgggtgtgaa tactaaccaa agcgaatgac
    35401 cttcgcaagg gcaattttat cgcattgtat caactgcgga aaaaaacagc aaaaccgatg
    35461 tgttcgctga gaaaaaaggc gacaatcgcc gcctttttag ccagttatct aacgctttaa
    35521 acggctttta ctgcatcggc gatgcggtgt gcaaattcag tcacctgcgc ttcgtcttcg
    35581 ccttccacca tcacgcgaat taacggttcg gtgccggatt tacgcagcaa cacgcgtcca
    35641 cggttgccca gcgcagcttc aacctctgcg gtcacggctt taactgactc atgctcaagt
    35701 ggatcgccgc tacctgcggt gtaacgtacg ttaaccagaa tctgcgggaa cattttcatg
    35761 ccgctgcaaa ggtcgtgcag gctcatatgg ttacgtgcca tcgccgccag cacctgcaag
    35821 ccagcaacga tgccgtcacc ggtagtagtt ttatccagca ggatcacatg accggaattc
    35881 tctgcaccga tacgccagcc tttctcctgc attttttcca gtacgtagcg gtcacccact
    35941 ttcgcgcgcg caaatggaat tcctaactgt ttcagcgcca gttcaagccc catgttgctc
    36001 atcaatgtac ccacagcgcc accacgcagc tggccctgac gaagaccttc acgcgcgatg
    36061 atatacatga tctgatcgcc atcgacttta ttgccttcat ggtcaaccat aatcacgcga
    36121 tcgccatcgc cgtcgaaggc aataccgaga tccgcttttt cagccagcac acgagcctgg
    36181 agcgcgcgaa cgtcggtagc ccccacttcg gcattgatgt ttacaccgtt tggctcacaa
    36241 ccgatagcga taacgttcgc ccccagttcg cgcagcacgt tcggcgcgat gtgataagtc
    36301 gcaccgtttg cacaatccac cacaatcttc agttcactga ggctaagttc gttcgggaac
    36361 gtggctttgc aaaactcgat atagcgaccc gcggcatcaa cgatacggct ggctttaccc
    36421 agttctgccg aatcaacgca gctgatctcc ttttccattt ccgcttcgat ggcctcttct
    36481 accgcatccg gcagtttggt gccgtcgata gagaagaatt taatgccatt atcgtagaac
    36541 gggttatgcg atgcagatat cacaattccg gcctctgcgc ggaaggtacg cgtcagataa
    36601 gccacggccg gtgttggcat cgggccagtg aagagtgcgg aaaggcccgc tgccgccaga
    36661 cccgcttcca gtgctgactc cagcatatag ccagaaatac gcgtgtcttt accaataata
    36721 atcttacggg agccgtggcg cgccagcact ttacccgcgg cccaacccag cttaagcaca
    36781 aaatcaggtg tgatcggcgc atcccctaca cgaccacgaa tcccatcggt accgaaatat
    36841 ttacgattac tcatagcgtt agttttcctt tgcagacaga gtggcttcca ccacccgcat
    36901 cgcttctacg gtttctttga cgtcatgaac acgaatgatg tgcgcgcctt gcattgcggc
    36961 aatgaccgca caggccagac taccgctcag gcgctcggac ggccccacgt tcagcagctg
    37021 cccaatcatc gattttcgtg acatacccac caacagcggc aggttgaaat ggtgaaattc
    37081 agccaggcgc gccagtaatg aatagttatg ggagagattt ttaccgaaac cgaatccggg
    37141 gtcgagcaac aatttctctt ttgcgatacc cgcctgctcg caacgtgcta tttgctcaat
    37201 aaagtagcga ttcacttctg caaagacatc gtcatacttc ggagcttcct gcatggtttt
    37261 tggatttccc tgcatatgca tcagacaaac cggtaaaccg gtttctgcag ccgcctccag
    37321 agcgccaggt tcggaaaggg agcggatatc attaataatg tgagcgccaa ctttcgctga
    37381 ctcacggatg acttctggtt tggatgtatc gaccgagatc cagacttcga agcgttgagc
    37441 aattgcctca accacaggaa taacacgttg caactcttct tcaacgctaa cttccgccgc
    37501 ccctgggcgc gtggactcgc caccaacgtc aatgatcgtc gcgccagcat tgatcatcag
    37561 attcgcatgt ttcaccgcat ctatcagcga gttatgcgtg ccaccatccg aaaaggaatc
    37621 aggcgtgacg ttgaggatcc ccattacgtg aggatggcta aggtccagtg aagtaccctg
    37681 ggcaaagagt ttcatgatgt tatccctggt atgaattgat aagaaaaaac cccggagcac
    37741 gccccggggt tttcggtaca aatacagtca tctgatgcgg gaacttactt gtcgcctaac
    37801 tgctctgaca tggtgttacc cgggttcggc gtacgcggtt catcaaccgg acgaggagcc
    37861 tttggactac cattgtcgcc agaattgtta gaagcgcctg gttcttccca gcccgctggc
    37921 ggacgtacat cgcgacgtgc catcaggtca tcaatctgcg gtgcgtcgat agtctcatat
    37981 ttcatgagag catctttcat cgcatgcaga atatccatat tgtcggtcag aagctgacgc
    38041 gcacgattat agttacgctc aatcagtgct ttcacttcct ggtcgatgat acgtgcagtt
    38101 tcatcggaca tatgtttcgc tttcgctacg ctacggccga ggaatacttc accttcttct
    38161 tccgcgtaca gcagtggacc caatttctca gagaagcccc actgggtcac catgttacgt
    38221 gccaggttgg tcgcaacttt aatatcgttg gacgcaccgg tagaaacatg ttccggcccg
    38281 tagatgatct cttctgccag acgaccaccg tacagcgtag aaatctggct ttccagtttc
    38341 tgacggctgg cgctgattgc gtcgccctca ggcaagaaga aagtcacacc cagcgcacga
    38401 ccgcgtggga taatcgtcac tttgtgcacc ggatcgtgtt ccggcaccag gcgaccgata
    38461 atcgcatgac ccgcttcgtg gtaagccgtt gattctttct gcgcttccgt catcaccatg
    38521 gagcgacgtt ccgcacccat catgattttg tctttcgctt tctcgaactc aaccatcgac
    38581 acaacgcgtt tgttgccacg agcagcgaac agtgccgctt cgttcaccag gttcgccagg
    38641 tcagcaccgg agaaaccagg agtaccacgg gcaatgattg ccgcgtcgat atcgggggcc
    38701 aatggtacgc gacgcatgtg aactttcagg atctgctcac gaccgcgaac atctggcaag
    38761 ccgaccacaa cctgacggtc gaaacggcca ggacgcagca gggccgggtc gagaacgtcc
    38821 ggacggttag tcgcggcgat aacgatgata ccttcgttac cttcgaagcc atccatctca
    38881 accagcatct ggttcagagt ctgttcacgt tcatcgtgac caccgcccag accagcgcca
    38941 cgctggcggc ctacggcgtc gatttcatcg ataaagatga tgcacggtgc cgctttcttc
    39001 gcctgttcga acatgtcacg aacacgggat gcacccacac cgacgaacat ttctacgaag
    39061 tcagaaccgg agatagtaaa gaacggaact ttcgcttcgc ctgcaatcgc tttcgccagc
    39121 aacgttttac cggtacccgg aggaccgacc atcaggacgc ctttcgggat cttaccgccg
    39181 agtttctgga agcggctcgg ctcgcgcaga tactctacca gttcagcaac ttcttctttt
    39241 gcttcgtcgc agcccgcaac gtcagcaaag gtcgttttga tctgatcttc cgtcagcatg
    39301 cgcgctttgc tcttaccaaa cgacatggca cctttgccac cgccgccctg catttgacgc
    39361 atgaagaaga tccagacacc aatcagcaac agcatcggga accaggagat gaagatagaa
    39421 gccagcaggc ttggttcttc aggcggttca ccgacaacct tgacgttctt ggtcaacagg
    39481 ttatccagta atttcggatc ctgaaccgga atgtaagtgg tataacggtt actatctttc
    39541 ttggtaacgt tgatttcacg tccgttgata cgcgcttcac gaacctggtc gttattgacc
    39601 tcttgtagga aggtagagta atccacctta cggccattag actcgctggg cccaaagctc
    39661 tggaatactg acatcagcac aacggcaatg accagccaga gtattaggtt tttcgccatg
    39721 tcactcaagg gattaacctc ttattacaac tgtgttaaaa acagcgtcag gatactctat
    39781 atccagcatc tttcaaactt tcgtctgaaa tctcccggtt agggtttacg cccggtcgct
    39841 acaatataca cttcccgcga acgtgcacga gaagagtccg gcttacgaac tttgaccttc
    39901 gtaaacaggg agcgaatttc ccttagatac tcatcgaaac cttcgccctg gaacaccttc
    39961 actacaaaac tgccacctgg cgctaataca tcacgacaca tttctagcgc cagttccacc
    40021 agatacatgg cacgggggat atccaccgcc ggtgttccgc tcatgtttgg tgccatatcg
    40081 gacatgacaa cctggacttt gctgtcgcca acgcgctcca gcagtgcttt catcaccagt
    40141 tcatcacgaa aatcgccctg aagaaagtcc acaccaacga taggatccat aggtaaaaga
    40201 tcgcaagcga tgatgcggcc tttgccgcca atttgggtga ccacatattg tgaccaacca
    40261 cccggagcag caccaaggtc gacaaccgtc attcccggtt taaagagttt gtcactttgc
    40321 tgtatttcat caagtttaaa ccaggcacgg gaacgtaacc cctttttctg tgcctgttga
    40381 acatatttat cgctaaagtg ttcctgaagc cagcggctgg agctggcaga acgcttctta
    40441 cctgtcattt aactttccca tggggataac tcatcgtaac caattgcgta aatttttacg
    40501 cgcctatttg gtgatatatg ggagatggcg gtagaatgac ccgttttcaa tcccaacgta
    40561 agcaaaaaat acgatgaatc tgagtactaa acaaaaacag cacctgaaag gtctggcaca
    40621 tccgctcaag ccagttgttc tgcttggcag taatggtttg accgaagggg tgctggccga
    40681 gattgaacaa gcgttagagc accatgaact catcaaggtg aaaatcgcca ccgaagatcg
    40741 cgaaactaaa accttgatcg tggaagctat cgtgcgcgaa accggcgcct gtaatgtaca
    40801 ggtcatcggt aaaacgctgg tgctttatcg cccaactaaa gaacgtaaaa tctcgctgcc
    40861 acgctaagat tatcctaaag ttacacacat ttgctgtgta aaacgagggg ttttccgcag
    40921 gcaggagagc aaaatgccac gctctgttcg ttgataaaag gccgcatagc ggcctttttc
    40981 ctttctttac aatacatcaa catcttgagt attgggtaat tcttacaggt attccacctt
    41041 aattacttca aattctactt cgccgcccgg cgttttgatg accacaacat catcttcttc
    41101 tttgccgatc aggccacgag caataggtga gtttacagaa atcaggtttt gtttaaagtc
    41161 agcttcgtca tcgccaacga tgcgataagt ctgttcttcg tcagaatcca gattcagcac
    41221 cgttacggta gcaccaaaaa taacgcgccc attgttgggc attttggtga catcaatcac
    41281 ctgcgcgttc gacagcttgg cttcgatgtc tttgatacgg ccttcgcaga agccctgctg
    41341 ttcacgagct gcgtggtatt cggcgttttc tttcaggtcg ccatgctcac gcgcttccgc
    41401 gatagcagca atgatttcag gacggcgcac agatttcaga aaatccagct cttcgcgtaa
    41461 tttttcagcg ccgcgtaagg tcatcggaat agcttgcatt tgttatacct cttgaatatt
    41521 cctgatgggg caagtcttca cccaccccgg ctgttcggcc tgcccggcat aactcctcac
    41581 cgggaccaga agcaaaaaaa taccgacccg ggtacaagtc ccaggtcagc tacaattcac
    41641 attttgatag tcattttacc ctgaagttcc cgaagggtca tcgtttactt tatagggcgt
    41701 tgcgccgtag tatgacggct cgattccagg ttgttagcgc gagattatgc gattttccag
    41761 atttatcatc ggattgacca gctgtatagc gttcagtgtt caggccgcga atgttgatga
    41821 gtacgttact caactccccg ctggtgccaa ccttgccctg atggtgcaaa aagtcggcgc
    41881 gtcggccccc gctattgatt accacagtca gcagatggcg ctgcccgcca gtacgcagaa
    41941 agtgattact gcgctggcgg cgttgattca actcggcccc gattttcgtt ttaccacgac
    42001 gcttgaaacc aaaggcaatg tggaaaacgg cgtacttaag ggtgacttag tggcgcgatt
    42061 tggtgccgat ccgacgttaa aacgtcagga tattcgcaat atggtcgcga ctttgaaaaa
    42121 atctggcgtc aaccaaatcg atggcaatgt gttgatagat acctccattt tcgccagcca
    42181 cgataaagcc cccggctggc catggaatga catgacacaa tgctttagcg ctccgcctgc
    42241 cgccgccata gttgaccgca actgtttctc cgtctcgctc tacagtgccc aaaaacccgg
    42301 tgatatggcc tttatacgcg tggcatctta ttaccccgtt acgatgttca gccaggtacg
    42361 caccctcccc cgtggttctg ccgaagcgca atactgcgaa ctggatgtgg tgccaggcga
    42421 cctgaaccgc tttacgctga cgggatgcct gccacaacgt tctgagccgc tcccgttggc
    42481 ttttgccgta caggatggag ccagctatgc cggggcaatt ctgaaagatg agttaaaaca
    42541 ggcgggtatc acctggagtg gaacactgct gcgccagact caggttaacg aacctggaac
    42601 ggtagttgcc agtaaacagt cggccccgct gcacgatctg cttaagatta tgctgaaaaa
    42661 gtcagacaac atgatcgccg atacggtttt ccgcatgata ggccatgcgc gcttcaatgt
    42721 gcctggaaca tggcgggccg ggtcggacgc cgtgcgtcag atcctgcgcc agcaagccgg
    42781 tgtcgatatt ggaaacacca ttattgccga tggttcaggg ctttcgcggc ataacctgat
    42841 tgcccccgcc accatgatgc aggtgctgca atacattgcc caacacgaca atgaacttaa
    42901 ctttatctcc atgctgccgc tggcgggcta tgacggctct ttgcagtacc gtgcaggtct
    42961 gcatcaggcg ggcgtggatg gcaaagtctc agcgaaaacc ggttcgttgc agggggtata
    43021 taacctggcg ggattcatta ccacagcgag cgggcaacga atggcgtttg tgcaatatct
    43081 ttctggctat gcagtagaac cggcggatca gcgtaatcgc cgtattccgt tagtgcgttt
    43141 cgaaagccgt ttgtataaag atatttacca gaacaattag tcaaaaagaa accccggcat
    43201 atggccgggg tttcagatta ttgacaaagt gcgctttgtt tatgccggat gcggcgtgaa
    43261 cgccttatcc ggcctacaaa atcgtgcaaa ttcaatatat tgcaatcatc ttgtaggcct
    43321 gataagtgta gcgcatcagg cttatttggc attggtcatt agtgattaac gcttgtaaat
    43381 gaactcaacg ccttcttcgt cgtcttcgtc ccagtcgtca tcccagtcct catcatcctc
    43441 ttcagcaatc tcttcaagct gctggcgatg ataatcatcc cacatgaatt cgactttctc
    43501 tggctgtttc gcttcttcag cctgcacgac cgggttttca atgataaagg tcatcacatc
    43561 ccagcagaga tctttcacgc ccagtccgct cgccgcagag atcagataat atttatcttc
    43621 ccagcccagc gcctcagcga tcgctttcgc tttctcttcg gcttctgcct tatccagcag
    43681 atcgatcttg ttgaacacta accaacgcgg tttcgccgcc agatcctggc tgtatttttc
    43741 cagctcgctg ataataatac gcgcgttttc aaccggatcg gtgccgtcaa tcggatcgat
    43801 atcgatgagg tgcaacagga cgcggcaacg ttccaggtgc ttcaggaagc ggatacccag
    43861 acctgcgcct tccgcagcgc cttcaatcag tcctggaata tcggcaacaa cgaagctctt
    43921 ttcgttgtcc attcgtacca cacccagact tggcaccaga gtggtaaacg gataatccgc
    43981 cactttcggt ttagccgccg ataccgcgcg aataaaggtc gatttccccg cgtttggcat
    44041 ccccaacata ccgacgtcag ccagcagcat cagctccagc agcagctcac gcttatcacc
    44101 cggcgtaccg ttggttttct gccgtggtgt acggttaacg gacgatttga aacgggtatt
    44161 gcccagaccg tgccagccgc ccttagcaac cagcagacgc tgaccgtgtt tagtcatatc
    44221 gcccatggtt tcaccagtac cctggtcgat tacacgcgta cctaccggca ctttaatcgt
    44281 gacgtcttta ccgcgcttac cggtacagtc gcggcttgcg ccgttttgac cgcgctctgc
    44341 acggaaagat ttttcaaaac gataatcgat aagcgtgttc aggttctcgt cggcttccat
    44401 ccatacgtca ccaccatcac cgccgtcgcc gccatccggg ccgcctttcg gaatatactt
    44461 ttcgcggcgg aagctcacgc aaccattacc gccatcacct gcaacgacca gaatcgatgc
    44521 ttcatcaaca aacttcattt tattctccgt aaatcattcg cctgcgcggg gttgcgatac
    44581 caccgttgta tgcttacgta atccgcccca aatacgatga ccaatggcgg aatacatcgc
    44641 gcccgcaacc acgacaaacg caccgagata acctaaaagg tttaacatcg gtctggcgaa
    44701 gaaatcgggc caggccagtg ataaaagatc tgaaaaaaac agcgtaaaca gtggggtgag
    44761 cgtgatgatc gcgctcacct gcgctgcctg ccagcgagcc atcgcttccg ccagggcgcc
    44821 atatcctacc aatgtattca gtccgcaaaa aattaaacat gcgagctgcc agtggctaag
    44881 ctgcgctatc actccaggct ttgccagagg gaagagcgcg attgtacata aagtgtacag
    44941 taaaaacaga atctgcggtg aggccagccg acgcaataaa accttttgcg ccacgccata
    45001 actcacccaa accgtcgccg caccgacccc aaagataact ccccaggtgt aatcggtgag
    45061 ctttgtaaat atctcgacca gactggtgtt aaaaaacatc accaggccgc tcaggagcat
    45121 caatgcccct acaacctgag tgctgcgcat tttctctttc aggataaata cgctggcaac
    45181 catcatgcca actggcgaga gttgcccaat cacctgcgaa gcggtcggac tcaggtattg
    45241 caaggatgag ctgaacagga tgaagttccc aaacagcccg gcggtcgcca ctgccaacaa
    45301 aatcaaccag cgtggcttac gaaacacgcg taatggcggc aacctcttct tcaccgcaag
    45361 aatggcacca aggccaatac tcgccatcaa gaaacggtaa aacacgattg tcggaggttc
    45421 catcacctcc agcacctgct tcattgcgat tggcaacgcc ccccagcaaa ttgctgtggt
    45481 gagcgccaaa agaatgccaa tgcctgcctg ctgcttcata ccgtattccc tgcaaagcgc
    45541 atttgcggat taacgtcgca ccatgctcat ttttaccggt tatcgaatgt aaaaagcccc
    45601 gcaacgtgtt gcggggcttt catccgttac cgggacgcga aaaacttatt cagcttcgat
    45661 gctgataaat ttacggtttt tcgggccttt aacttcgaat ttcactttac cgtctgcttt
    45721 agcaaacaga gtgtggtcac gaccgcaacc tacgttagcg ccagcgtgga atttggtacc
    45781 acgttgacga acgatgatgc tacccgccag aacggattcg ccaccgaaac gcttaacgcc
    45841 caggcgttta gcttctgaat cgcgaccgtt acgtgtggag ccgccagcct ttttatgtgc
    45901 catttgaaat ctctcctcag gtcttaggcg ctgatgccag taattttcac atcagtgaac
    45961 cactgacgat ggccctgctg cttacgatag tgtttacgac gacgaaactt aacgatttta
    46021 actttctcgc cacgaccgtg agcaacaact tcagctttga ttacgccgcc atcaacgaaa
    46081 ggaacgccga ttttgacttc ttcaccgttt gcgatcatca gcacttcagc gaactcaaca
    46141 gtttcgccag ttgcgatgtc cagcttttcc aggcgaacgg tctgaccttc gcttactcgg
    46201 tgttgtttac caccactttg gaaaaccgcg tacatataaa actccgcttc cgcgcacacc
    46261 ttttcaatga ttcagagtgc gctataaata ttcacaatag ggcgcgaata ttacgcaaaa
    46321 cgcacgcctt tgacaagtgc tacagtcaat acacgaagaa aaaaaacaca acttgtacgg
    46381 taacgtttat ctgtgccatt ttttcagtac aatcacccta tattcctaac cataaaccct
    46441 aagttgcctt tgttcacagt aaggtaatcg gggcgaaaag cccggctttt gcgatgaatt
    46501 tagaaaaaat caatgagtta accgcgcaag atatggcggg tgttaatgcg gcaatccttg
    46561 agcagcttaa ttccgacgtc caactgatca atcagttagg ctattacatc gtcagcggcg
    46621 gcggtaaacg tattcgtccg atgattgctg tactggctgc acgagctgtt ggctatgagg
    46681 gaaatgcgca tgtcaccatt gctgccttga tcgagtttat ccacacggcg actctgctac
    46741 acgacgacgt tgtggatgaa tcagatatgc gcaggggtaa agctaccgcc aacgctgcat
    46801 ttggcaatgc cgccagcgtg ctggtaggcg attttattta tacccgcgcg ttccagatga
    46861 tgaccagcct cggttcgctc aaagtgctgg aagtcatgtc agaagccgta aacgtcatcg
    46921 cagaaggtga agttctgcaa ctgatgaacg ttaacgatcc ggacatcact gaagaaaact
    46981 acatgcgcgt tatctatagc aaaaccgcgc gtctgtttga ggctgccgcg cagtgttccg
    47041 ggattctggc tggctgtacg ccggaggagg agaaaggcct gcaggattat gggcgctatc
    47101 tcggcactgc tttccagttg atcgacgatt tactcgatta caatgccgat ggcgaacagt
    47161 taggtaaaaa cgtcggcgac gatctgaacg aaggtaaacc gacgctgccg ctgctgcatg
    47221 cgatgcatca tggcacacca gaacaggcac agatgatccg taccgccatc gaacagggta
    47281 acggtcgcca tcttctggaa ccggttctgg aagcaatgaa cgcttgtgga tctcttgaat
    47341 ggacgcgtca gcgcgccgag gaagaagcag acaaagccat cgcagcgtta caggtgctcc
    47401 cggacacccc ttggcgagaa gcactcatcg gcctcgcgca catcgctgtt caacgcgatc
    47461 gttaatcccc tcccctcatc ccgcgcagcg cgcggggtgg ttccagtaaa ttccataaaa
    47521 acacttactc agctctataa cacctgaaaa acaggctgtg catctttata tattctgaat
    47581 attcacactc cttacatgaa ctttttagag caatcggcca tcaggagtat agtgatgctc
    47641 gacagaagaa gtgttctgaa tgaaagcgac taacttaagg agtgaggaaa gatggaaagt
    47701 aatttcattg actggcatcc cgctgacatc attgcgggtt tgcgcaagaa gggaacgtca
    47761 atggcggcgg agtctcgcag aaatggtttg agttcctcaa cgctggcgaa tgcattatcg
    47821 cgcccatggc cgaaaggaga gatgattatt gcgaaagccc tgggaactga cccctgggtt
    47881 atctggccat cacgctacca tgatccgcag acccatgagt ttatcgacag aacgcagttg
    47941 atgcgtagct acactaaacc gaaaaaatga gtggtctggc ggtagccccg cgaacggggc
    48001 tgccagctct cagacgatta ttcgcctttc acacgctcaa tatttgcacc taaagcgcgc
    48061 agtttgtctt caatgcgttc gtagccacga tcgatgtgat aaatacgatc aaccaccgtc
    48121 gtcccttccg caatacagcc agccagcacc aggcttgctg atgcacgcag atcggttgcc
    48181 ataacctgtg cgccagaaag tttttcaaca ccgtgacaaa taacggtatt gctttcgatt
    48241 tcggcatgcg cgcccatacg gctaagctcc ggcacatgca taaagcggtt ttcaaagacc
    48301 gtttcggtga tgaatccggt cccttctgcc accaggttca acagcgtgaa ctgggcctgc
    48361 atatcggtcg ggaatgccgg atgcggcgcg gtacgtacgt taacagcctt cggacgtttg
    48421 ccatgcatat ccaggctaat ccagtcttcg ccgacttcga tgtccgctcc agcatcacgc
    48481 agtttcgcca gcacggcgtc cagagtatct ggctgcgcgt tacggcagat aattttgccg
    48541 cgagaaatcg ccgccgccac caggaaagta ccggtttcga tacgatccgg tagcacgcga
    48601 tagacaccgc cgcctaaacg ttccacacct tcgatgacga tacgatcggt gccctgaccg
    48661 ctaattttcg cacccagcgt aatcaggaag ttcgcggtat cgacgatttc cggttcacgc
    48721 gctgcgtttt caataatcgt ggtaccttcc gccagggttg cagcacacat gatggtcacc
    48781 gttgcgccaa cgctgacttt atccatcacg atatgtgcgc ctttcagacg accatcgacg
    48841 gaagctttaa cgtaaccttc ttccagtttg atggtcgcgc ctaattgttc gaggccagaa
    48901 atgtgtagat caaccggacg cgcaccgatc gtacaaccgc caggcagtga aacttgcccc
    48961 tgaccaaagc gcgctaccag cggccccagt gcccagatag aagcacgcat ggttttaacc
    49021 agatcgtaag gtgcgcagaa tacattaacg tcgcgggcat caatatgcac agaaccatta
    49081 cgttctactt tcgcacccag ctggcttagc agcttcattg atgtatcgac gtctttcagt
    49141 ttcgggacgt tctggatctc taccggttct tccgccagta gagcggcaaa aaggataggc
    49201 agagcagcat ttttagcgcc ggaaattgtg acttcgccct ggagcttcgt tggcccctga
    49261 acacgaaatt tatccattta gtttgttctc agttaacaat tcatatccgc taccggcgaa
    49321 tcgcccatag ctcaaaagcc gttcagtttg cgatcgcgcg cccactccgc aggggtatac
    49381 gctttgatcg acacagcatg aatgcggtta tccgcaatat attccatcag cgggccatag
    49441 accgtctgct gttttttaac ccgactcatg ccgtcaaaca actcacccac ggcaataacc
    49501 tgaaagtggc tgccatcgcc ggaaacgtgg acttcctgga gggagagagc gttcatcaac
    49561 acgctctgaa tttcattatt ttccatggga tcttcaatca tcagttaata aaccagcgaa
    49621 acatcttaga gcaaagttgc gctggcataa ataagcaaaa agcctcgctg ataaatcaga
    49681 caaggctcga cttgcaggca ggtttgccgg acaggcggtt aacgccatat ccggcctgaa
    49741 aaaatttaac gaggcagaac atcagcaggc aaattataca atttcgccag ggtatacact
    49801 ttgtcgttta ccccctgaag cgtcacattg ttgccctgct ttttcgccag atcgataaga
    49861 tggagcagca gtgccagtcc ccccgtatcc acgcgggaga cacggctaag atcgatgcag
    49921 gtaatcccct tcaccgcttc ctcacgcatt tcccaaagcg gtagcaaaac gtcctgatcc
    49981 agctctccgg ataacgccag cgtgtcaccc gtctgcatcc agctcagtga ctcgctcatt
    50041 attttttctc ttccagagtg attttctgtt gagaaatcga tttcagttgc gctgtcaggc
    50101 cgtcgatacc tttggtacgc agcagcgttc cccactcgtt ttgtttggtg gtgatcatac
    50161 tgacgccttc agcaatcatg tcgtaagcct gccagttgcc cgtctgggag tttttacgcc
    50221 actggaagtc cagacgcacc ggcggacggc cattcgggtc aataatggta acgcgaatag
    50281 gcacaatggt tttatcgccc agcggctgtt ctggcgcaat ctgataggtt tgaccgtgat
    50341 acatcgccag cgcctgaccg taagcctgct tcaggtactc acggaaagcg gcaaagtagg
    50401 cttcacgttg agcaggggtc gcactcttgt aatactggcc cagcaccagc gcaccggcgt
    50461 atttcacctg tacgtatggc agcagttcct gatcaacaat ggtacgcaga taatccgggt
    50521 tggcccgaat ttgcggttgc tcattcttca ggcgatcgaa cgttttctgc gccgcctcgt
    50581 ccatcagctt atacggattg gtctggtctg ccgcggttgc cgcactcaga ggtgcaatca
    50641 ccagcaaagc gaccatcatt aaacgtttaa acatgcgtcg gttctcctga aattatttcg
    50701 ttgtacccac aggttcagtg gtttcattat tacctggcgc agcagctggc gcatcgccac
    50761 tattcttatt gtcatcgcct ttactaccgt aaaggaactg accaatgaga tcttccagca
    50821 ccatcgcaga cttagtgtcc tgaattgtat cgccatcctt caggatagca gtccccagtt
    50881 ccgggtcttc aaaaccgacg tttaatgcca gatattgttc ccccagcagg ccggaagtac
    50941 gaatgctcag cgaactggta tctggaatgt ggttataacg ttgttcaatt tccagcgtta
    51001 cgcgcggcag ataggttttc gggtccagcg taatatccgc cacccgaccc acaacaacgc
    51061 caccaatact gaccggagag cgcgctttca ggccgccaat gttatcgaac gtcgcataaa
    51121 gcgtgtaggt cggttcagta cgtatggacg tcacgttcgc cgccttcagg caaacaaaca
    51181 gcgccgccag cagtgctgct aataaaaaga tacccaccca aatttcattt tttttcgttt
    51241 gcatgaactc aattcccaaa catcaatgcg gtcagcacaa aatccagccc cagaacagcc
    51301 agagacgagt ggacaacggt gcgagtggtt gcccggctaa tcccggcaga cgtcgggatg
    51361 gcgtcgtagc cgttaaacaa cgaaatccac gtcaccgtga tggcgaacac cacgctctta
    51421 atcagacagt tgaccagatc catacgccag tcgacggcat tttgcattgc cgaccagaag
    51481 aacccgctat caatgccttt ccaactgacg ccgaccagag atccgcccca gatccccacg
    51541 gcgacgaaaa taaccgtcaa cagtggtaat gaaataaccc cagcccagaa acggggagaa
    51601 ataacccgac gcagcggatc caccgccatc atctccatac tggagagttg ctctgtagcg
    51661 cgcatcaggc cgatttctgc ggttagcgcc gaaccagcac gcccggcaaa caacaacgcg
    51721 gcaaccaccg gccccagttc acgcagtagc gataacgcca ccagcatacc cagactggtt
    51781 tccgcactat aagtggtcag aaccagataa ccttgcagcc ccaacaccat tccgatgaac
    51841 acgccagaaa ccacaataat cagcatcgac aggacgccga cattatagag ctggcgcacc
    51901 agcagcggcg catgtttgcg aaattccggt ttgccgacca gcgcattgaa taacattaac
    51961 ccggcccgcc cgaacgttct cagggtttta atccctttat gtccgagcga cgccagcgca
    52021 tttaacagca tgagtggctt aactccctgg taaaagatca gcgtgataat cgccggcagg
    52081 atagcggaac ggaacaggcc cgtcagctat cccgtccaga aactgacgta cgcgcggatc
    52141 aggattcgcc tgcaacgcct gggcactgcc atgagcgaca atttttttgt ccgccaggat
    52201 ccaggcgtga tccgcaatac ttaacacttc cggcacatcg tgagaaacca ccacacaagt
    52261 cacgcccagc gcgctgttca gctcagaaat cagcttcacc agtacgccca tggtgatggg
    52321 atcttgccca acaaaaggtt catcaaacat gatgagatcc ggctccagcg caatcgcacg
    52381 cgccagcgct gcacgccgcg ccatcccacc ggaaagttca gaaggcatta gtttagccgc
    52441 tccacgcagc cccacggcct ccagcttcat catcaccgta ctatgcaaca atggcgcggg
    52501 aagttgggta tgttcgcgca gtggataggc gacgttgtca aatacgttca tatcagtgaa
    52561 caacgccccg gactgaaata acatgctcat ccgtttgcgc actgtataca ggcgcgaacg
    52621 agacatcgcc ggaatattct caccatcgaa aaggatctca ccatgatctg gtgcgatttg
    52681 cccgccaatc agacggagta gcgtcgtttt accgatgccc gatggcccca tgatcgccgt
    52741 gatcttccct cgcggcacgg tcagggaaat attatcgaag atgcagcgat tgccacgcgt
    52801 aaaactgaca tcgcgcatat cgactaaatt cgccacagac tgctccataa ttcacccttc
    52861 gtcttgcgtt gattttctaa gcatggcgct caatttaacc ttgaacccaa catatttaca
    52921 gaatattacc cgccgtggtt agcgaaagct ggcatttgtt ttacttttta gccgcataaa
    52981 gtcaaaatta agaatccgtt acggctttct gaaaatcttc agcggaccgg cgagtatacc
    53041 tgaagaaagg acgttagatg cttttagcta cggcactgtt aattgttggt ttacttttgg
    53101 tcgtttacag tgccgaccgc ctggtttttg ccgcgtctat tctttgccga acctttggca
    53161 tcccgccgct gatcatcggc atgacggtgg tcagtattgg tacatcgtta ccagaaatca
    53221 tcgtctcgct tgccgcgtct ctgcacgaac aacgcgattt agccgttggt acagccctcg
    53281 gctcaaacat tatcaatata ttgctgatcc tcggtctggc tgcgctggtt cgtcctttta
    53341 ccgtccattc tgatgttcta cgccgtgaat tacccttaat gttgttggtc agcgtggtgg
    53401 ccggttccgt actctatgac ggacaactta gtcgaagcga tggtatcttt ctcctctttc
    53461 tggctgtgct atggctgctg ttcattgtta aacttgcacg tcaggctgaa cgtcagggga
    53521 ctgacagcct gaccagagag cagcttgcag agctgccgcg tgacggcgga ttgcccgtcg
    53581 cgtttttatg gctcggcatt gcgcttatca tcatgccagt ggccacgcgg atggtggttg
    53641 ataacgccac ggtgctggcg aattactttg ccatcagcga gttgacgatg ggtctgacgg
    53701 caattgctat cggaaccagc ctgccggaac tggcaaccgc aatagcgggg gttcgcaaag
    53761 gtgaaaacga cattgctgtc ggaaatatca ttggcgcaaa catttttaat attgtcatcg
    53821 tgttgggttt acccgcgctg ataacgccag gagagattga tccactggcg tacagtcgtg
    53881 actacagcgt gatgttgctg gtgagcatta tttttgcgtt gctgtgctgg cggcgctccc
    53941 cgcaaccggg ccgtggtgta ggggtattat taactggcgg atttatcgta tggctggcga
    54001 tgttgtacag gttatcgcca atactcgttg aataactgga aacgcattat gtcgcacgta
    54061 gagttacaac cgggttttga ctttcagcaa gcaggtaaag aagtcctggc gattgaacgt
    54121 gaatgcctgg cggagcttga tcaatacatc aatcagaatt tcacgcttgc ttgtgaaaag
    54181 atgttctggt gtaaagggaa agttgtcgtc atggggatgg ggaaatcggg gcacatcggg
    54241 cgcaaaatgg cggcaacgtt tgccagcacc ggtacacctt catttttcgt ccatcctggt
    54301 gaagccgcgc atggtgattt aggcatggtt accccacagg atgtggtgat tgctatctct
    54361 aactcaggtg aatccagcga aatcacggcc ttaattccag tgcttaagcg tcttcacgta
    54421 ccgttaatct gcatcaccgg tcgcccggag agcagcatgg cgcgcgccgc agatgtgcat
    54481 ctgtgtgtta aagtagcgaa agaagcctgt ccgttagggc tggcaccgac cagcagcacc
    54541 accgccacgc tggttatggg cgatgccctc gctgtcgcgc tgttaaaagc acgcggcttt
    54601 actgctgaag attttgcgct ctcacaccca ggcggcgcac tgggtcgtaa acttctgctg
    54661 cgcgtaaacg atattatgca tacgggcgat gagatcccgc atgttaagaa aacggccagt
    54721 ctgcgtgacg cattgctgga agttacccgc aaaaatcttg gtatgactgt catttgcgat
    54781 gacaatatga tgattgaagg catctttacc gacggtgatt tacgccgtgt cttcgatatg
    54841 ggcgtggatg ttcgtcagtt aagtattgcc gatgtgatga cgccgggggg aatacgtgtg
    54901 cgccctggca ttctggccgt tgaggcactg aacttaatgc agtcccgcca tatcacctcc
    54961 gtgatggttg ccgatggcga ccatttactc ggtgtgttac atatgcatga tttactgcgt
    55021 gcaggcgtag tgtaaagatt caaggataaa caacaatgag caaagcaggt gcgtcgcttg
    55081 cgacctgtta cggctctgtc agcgccgacg ttatggcaaa agcagagaac attcgtctgc
    55141 tgatcctcga cgttgatggc gtactgtcag atggcctgat ttatatgggc aataacggcg
    55201 aagagctgaa agcgttcaat gttcgtgacg gttatggcat tcgttgtgcg ctcacctctg
    55261 atattgaagt cgctatcatt accgggcgaa aggctaaact ggtagaagat cgttgtgcca
    55321 cattggggat cactcacttg tatcaggggc agtcaaacaa actgatcgcc tttagcgatt
    55381 tgctggaaaa actggcgatt gccccggaaa atgtggctta tgtcggcgat gatctcatcg
    55441 actggccggt aatggaaaaa gtgggtttaa gcgtcgccgt ggccgatgcg catccactgt
    55501 tgatcccgcg cgccgattac gtgacgcgca ttgctggcgg tcgtggcgca gtgcgcgaag
    55561 tttgcgactt attactcctg gcgcagggca aactggatga agccaaaggg caatcgatat
    55621 gagtaaagcc agacgttggg ttatcattgt gctatcactg gcggttctgg tgatgatcgg
    55681 cattaatatg gccgaaaaag acgataccgc ccaggtggtc gtcaacaaca atgatcccac
    55741 ctataaaagc gagcatacgg acacgctcgt ctataaccca gaaggggcac taagctatcg
    55801 attgattgct caacacgttg aatattattc cgatcaggcc gtttcgtggt ttacgcagcc
    55861 ggtacttacc acgtttgata aggataaaat cccaacatgg tccgtaaaag cagataaagc
    55921 caagctgacc aatgaccgga tgctctattt atatggacac gttgaagtca acgcacttgt
    55981 gccagactct caacttcgca gaatcacgac ggataacgcg cagatcaatc tggtgactca
    56041 ggatgttacc tctgaagacc tcgtcacgtt atacggaaca acatttaact ccagcggtct
    56101 gaaaatgcgc ggcaacttac gcagcaagaa cgccgagctg attgaaaagg ttagaacatc
    56161 ctatgaaatt caaaacaaac aaactcagcc ttaatcttgt gcttgccagc tcacttctgg
    56221 ccgccagcat tccggcattt gccgtaaccg gagacactga tcagccgatc cacattgaat
    56281 cggaccagca atctcttgat atgcaaggca acgtggttac ctttaccggt aatgtcatcg
    56341 tcacccaggg caccatcaaa attaatgccg acaaagtggt cgttacccgt ccgggcggcg
    56401 aacaaggtaa agaagtgatt gacggctacg gtaaaccggc aacgttctac cagatgcagg
    56461 acaacggtaa acccgttgaa ggtcacgctt cccagatgca ctacgaactg gcaaaagatt
    56521 ttgtcgttct gacgggtaat gcttatctgc agcaggtcga tagcaacatt aagggcgata
    56581 agatcactta cctggtaaaa gagcagaaaa tgcaggcttt cagcgacaaa ggcaagcgcg
    56641 taaccaccgt tctggtgccg tcgcagctgc aggataaaaa caacaaaggc cagaccccgg
    56701 cacagaagaa gggtaattaa ttcgttatgg caacattaac tgcaaagaac cttgcaaaag
    56761 cctataaagg ccgtcgcgtg gtagaagacg tcagcctgac cgtcaactcc ggggaaattg
    56821 tcggtctgct ggggccaaac ggtgccggta agaccaccac tttctatatg gttgtaggca
    56881 ttgtgccgcg cgatgcgggc aacatcatta ttgatgatga cgatatcagt ctgctgcctc
    56941 tgcatgcacg cgcgcgccgc ggtatcggct atctgccaca ggaagcctcc attttccgtc
    57001 gcctcagcgt ttacgataac ctgatggcgg tactgcaaat tcgtgacgac ttgtctgctg
    57061 aacaacgtga agaccgcgcg aacgagctga tggaagagtt tcacattgag cacctgcgtg
    57121 acagcatggg gcagtcactc tccgggggtg aacgtcgccg tgtagaaatt gcccgcgcac
    57181 tggctgcgaa tccgaaattt attctgctcg acgaaccgtt tgccggtgtt gacccgatct
    57241 cggttatcga cattaaacgc atcattgagc acctgcgcga cagcggcctg ggcgtgctga
    57301 tcactgacca caacgtgcgt gaaacactgg cggtttgtga acgcgcttat atcgtcagtc
    57361 aggggcattt gatcgcccac ggcacgccta cagaaatctt acaagacgaa cacgttaagc
    57421 gtgtatacct tggggaagac ttcagactct gatagggtag aagtttgcga cgttttagca
    57481 ggagagtacg attctgaaca tgaagcaagg tttgcaactc aggcttagcc aacaactggc
    57541 gatgacgcca cagctccaac aggcaattcg tctgttgcag ttgtcgacgc tggaacttca
    57601 gcaggagcta cagcaggcgc tggagagtaa tccgctgctt gagcaaatcg acactcatga
    57661 agaaatcgac acccgcgaaa cgcaagacag tgaaacgctg gacaccgccg acgcgctcga
    57721 acaaaaagag atgccggaag agctgccgct cgatgccagt tgggacacca tttacaccgc
    57781 tggtacacca tccggcacca gcggtgacta cattgacgat gagctgccgg tctatcaggg
    57841 cgaaacgacg cagaccttgc aggattacct gatgtggcag gtcgagctga caccgttttc
    57901 cgacactgac cgcgctattg ctacctctat cgtcgatgcc gttgatgaca ccggttatct
    57961 gactgtcccg ctggaagata ttctcgaaag tatgggcgat gaagagatcg acatcgacga
    58021 ggttgaagcc gtccttaagc ggatccaacg gtttgatccg gtcggtgtag cggcaaaaga
    58081 tctgcgtgac tgcctgctaa tccaactctc ccaattcgat aaaaccacgc cgtggctgga
    58141 agaggccaga ttgatcatta gcgatcatct cgatctgtta gccaatcacg acttccgcac
    58201 tttaatgcgc gtcacgcgtc tgaaagaaga tgtgctgaaa gaagccgtca atctgatcca
    58261 gtcgctcgat ccgcgccccg ggcagtcgat ccagactggc gaacctgagt atgtcattcc
    58321 agatgtgctg gtgcgtaagc ataacggtca ctggacagta gaactcaaca gtgacagcat
    58381 tccgcgtctg caaatcaacc agcactacgc ctcgatgtgc aataacgctc gcaatgatgg
    58441 tgacagccag tttatccgca gcaatctgca ggatgccaaa tggttgatca agagtctgga
    58501 aagccgtaac gatacgctac tgcgcgtgag tcgctgtatc gttgaacagc agcaagcctt
    58561 ctttgagcaa ggcgaagaat atatgaaacc gatggtactg gccgatatcg cccaggctgt
    58621 cgaaatgcat gaatcgacga tatctcgcgt gaccacgcaa aaatacctgc atagtccacg
    58681 aggcattttt gaactgaagt atttcttttc cagtcacgtc aataccgagg gcggcggcga
    58741 agcttcctcc acggcgattc gtgcgctggt gaagaaatta atcgcggcgg aaaacccagc
    58801 gaaaccgttg agcgacagca agttaacctc tttgctgtcg gaacaaggta tcatggtggc
    58861 acgccgcact gttgcgaagt accgagagtc tttatccatt ccgccgtcaa accagcgtaa
    58921 acagctcgtt tgacccaacc gataaggaag acactatgca gctcaacatt accggaaata
    58981 acgtcgagat caccgaggca ctgcgcgaat ttgttacagc caaatttgcc aaacttgagc
    59041 aatattttga ccgaatcaac caggtctatg ttgttctgaa agtggagaaa gtcacccaca
    59101 cctcagatgc aacactgcat gtaaacggtg gtgaaattca tgccagcgcg gaaggtcagg
    59161 acatgtacgc tgccattgat ggcttaattg ataagctggc acgccagttg accaaacata
    59221 aagataaact gaaacaacac taattgtccg ggcaattagc atgtgcatgg cggcctgttg
    59281 tgcggcacaa cgggccattt gtacggttaa tgctccgagc ctgttccact gtttgagtgg
    59341 gcaggttctt aggtgaaatt atgacaaata atgatacaac tctacagctt agcagtgttc
    59401 ttaacaggga atgtacgcga agccgcgtcc actgtcagag caaaaaacgc gccctggaaa
    59461 tcatcagcga gctggcggcg aaacaactta gcctgccgcc tcaggtggtt tttgaagcta
    59521 tcctgacgcg ggaaaaaatg ggcagtaccg gtatcggcaa tggtattgcc attccgcatg
    59581 gcaaactgga agaagatact ctgcgcgccg ttggcgtttt cgttcagctc gaaacgccta
    59641 tagctttcga tgccatcgac aaccaaccgg tggacttact ttttgccctg ctggtgccgg
    59701 cagaccaaac taaaacgcac ctgcatactc tgtcgctggt ggcgaaacgt ctggcggaca
    59761 aaaccatctg ccgccgttta cgcgcagccc agagcgatga agagctgtat caaatcatta
    59821 cggataccga aggtactccg gatgaagcgt agttattcgg taatgtctct tttagacgtt
    59881 gtgaggagaa acagtacatg gtactgatga tcgtcagcgg acgttcaggt tcaggtaaat
    59941 ctgtcgccct gcgtgcgctg gaagatatgg gtttttactg cgtggataac cttcccgtag
    60001 tgttgttacc cgatctggct cgaactctgg ccgatcgcga gatttctgcc gccgtcagca
    60061 ttgatgttcg taatatgccg gagtcaccag aaatattcga acaggcgatg agtaacctgc
    60121 ctgacgcttt ctcaccgcaa ctactgttcc tggatgccga ccgtaatacc ttaattcgtc
    60181 gttacagtga cacgcgccga ctgcatccgc tttccagcaa aaacctgtcg ctggaaagtg
    60241 ctatcgacaa agaaagcgat ttgctggagc ctctgcgttc gcgagcggat ctgattgtcg
    60301 atacctcaga aatgtccgtt cacgagctgg ctgaaatgct gcgtacccgt ctgctgggta
    60361 aacgtgaacg cgaactgacc atggtctttg agtctttcgg cttcaaacac ggtatcccta
    60421 tcgatgcaga ttacgtcttt gacgtgcgct tcttgccgaa cccgcactgg gatccgaaac
    60481 tgcgtccaat gacaggtctt gataaacctg tcgccgcgtt cctcgaccgc cacacagaag
    60541 tacacaattt tatctaccag acgcgaagct atcttgaact atggttacct atgctggaaa
    60601 ccaacaaccg tagctacttg acggtcgcca ttggttgtac cggcggaaag caccgttcgg
    60661 tgtatattgc agagcaactg gcagactact tccgctcgcg cggtaaaaac gtccagtcac
    60721 gccatcgtac gctggaaaaa cgtaaaccat gaccgtcaag caaactgttg aaatcacaaa
    60781 caagctgggc atgcatgccc ggcctgcaat gaagctgttt gaattaatgc agggttttga
    60841 cgctgaagtg ctgttacgta atgacgaagg caccgaggcc gaagccaaca gcgttattgc
    60901 gctgctgatg ttggattctg ccaaaggacg gcagattgaa gttgaagcga ccggcccaca
    60961 ggaagaggaa gcactggccg ccgttatcgc cctctttaac tctggttttg atgaagatta
    61021 atcttcatca ctttgacata caactccctt caaactcccc ccgacaataa gaaaatcacg
    61081 tactgaaatc gttctcaatc aacgtcattt gtacattttg tgcgcttttc actttcagaa
    61141 gaaccttaag aaaaccttaa gaggcattgt ttaggttttg tttaagttaa tcgaccatac
    61201 tggagatcgt cagaaaatat ttccaggaga tggcatgatt cgcttatctg aacaaagtcc
    61261 cctgggcacc ggaagacatc ggaaatgtta tgcgcatccg gaagatgccc aacgctgtat
    61321 caagattgtc taccatcgtg gcgatggtgg cgataaagag atccgccgtg agttaaagta
    61381 ctacgcgcat cttggtcgcc ggttaaaaga ctggagtgga ataccgcgct atcacggtac
    61441 cgttgagacg gattgcggaa ccggttatgt ctacgatgtg attgcagatt ttgacggcaa
    61501 accctccatt acgctgaccg aatttgctga acaatgccgt tacgaagaag acattgcaca
    61561 actgcgccag ctactgaaac agctgaagcg ttatttgcag gataaccgta tcgtgacgat
    61621 gtcgctgaag ccgcagaata tcctctgtca ccgcatcagt gagtcagagg ttatcccggt
    61681 cgtctgtgac aacattggtg aaagcacatt gattccactg gcgacctggt cgaagtggtg
    61741 ttgtctgcgt aagcaagagc ggttatggaa acgatttatc gcccaaccgg cgctggctat
    61801 tgccctgcag aaagatctgc aacctcgtga gagcaaaacg ctggccctca cttcgcgcga
    61861 agcttaatcc agctggtgtt gttgcataaa cggctcaccg cctaactgat acatctgccg
    61921 taaaatccac gcctgacggc tacgcacgta gcccgatggc gaggagactt tgaaacgaag
    61981 tggattaggt aatacagctg ccagtaatgc agcttccgac cgggtaagtt tgctcgcggg
    62041 tttgtggaaa taacgttgtg ccgcagcttc gacgccaaac acaccgtcgc caaattcggc
    62101 gatattcagg taaaccgtca ggatacgctt tttgctccag actgtttcta tccccagcgt
    62161 taatccagct tccagccctt ttcgtaccca gctacgccca tcccataaaa agagattttt
    62221 ggccgtctgc tgagaaatcg ttgaagcacc gcgaatacgg ttttcattgc gctcgttgtg
    62281 cgccagggct ttctcaatgg aagcgacatc aaagccccag tgctcaggaa atttctgatc
    62341 ttctgcggca atcaccgcca gtcccatcca cggcgagatt tgatccatac tgacccagtc
    62401 agaatgtgcc acgtaacgaa aattgccatg cagccaggcg ctgacctgtc gctcgaccat
    62461 taccgctgag aagggaacag gcgcaacact aaacaacgcg atgcccccgc cccagaaaac
    62521 ggcgaggaca accattaacc gcaaaagaaa acggcgaacg aaactaaaca ccgttaagcg
    62581 gcttttactc attcagccag aaccagcacg cgggaaacca gcttatcaat gccgctcgcc
    62641 gcttctgcaa tgttctgcgc cagcatatat gctggggtgg tgacaatctt attgtcttca
    62701 tcaaccacga tatcatcgac aggacagggc acatgctccg cgcccatctc ttccagcact
    62761 tctgcggtat cgatatcagt accgatggtc aaacgcagcg ggaaatcgaa aattttcggc
    62821 agcatcgccg gggcaataca cataaaacca agcggttttc cggcctgatg catcgcttgt
    62881 gccagcgcct ttaattcacg gtcaacggtg cattcgctac caagagtggc aaaattgctt
    62941 aaattcttcg ccgcgccaaa cccccccggc acaatcaacg catccagttc agcggcatcg
    63001 gcctgggcca gaggacggat ttcaccgcgc gttattcgtg ccgcttcaat cagcacgttg
    63061 cgcgtttccg tcatcgcttc gccagttaaa tggttgataa catcaacctg ctgcttatcc
    63121 ggtgcaaagc agaccgcctg cgcaccgctg cgtgaaatag ctaacaacgt caacaccgct
    63181 tcatgaattt cagaaccgtc atagacgccg catccgctca gaattacgcc aattttcttc
    63241 attgtgatca tcctttcgca attgactgaa acacattaat ttttttaata aaaatggtac
    63301 gcatcacaca tttaactgat tcatgtaaca aatcatttaa gttttgctat cttaactgcg
    63361 tgcggcctga aaaacagtgc tgtgcccttg taactcatca taataattta cggcgcagcc
    63421 aagatttccc tggtgttggc gcagtattcg cgcaccccgg tctagccggg gtcatttttt
    63481 agtggctttt gccacccacg ctttcagcac ttctacgtcg tgacgccact cttctttcat
    63541 ctcttcaatc cattcaccga cgttatcttc ccaggcagga aggtccggag actgaatttg
    63601 ctgacccagc tgttgcagat ggcgtaaccc cactgacccc gccgcacctt taattttatg
    63661 tccttcctct acaatgcctt ttttatcctg cgccgtcaga ttcgactcca gcacgctgac
    63721 atagcccggc atcatcttct caaacaccgc taacccgtcg gtgatcagct tcggtcctac
    63781 aagttcgaga tactgttcca gcatgggaat atcgagcaat gcttctgatt tactgttctc
    63841 ttctgtcgtc accgtactct cctcatcatc ctgggtatcc cagaatttct tgatcatcgc
    63901 ggttagcgcc ggaaccgaaa gcggcttact cagcacatca tccattccag cattgaggta
    63961 ctcttgtttg tctttcagca cgttagcggt taaggccacc agcggcggta aatcctcgcg
    64021 cggataacgc ttcgtcagtt cacgagagat atccagcccg gtcatatctg gcaactgaat
    64081 atccagcaac accaggtcgt attcgcccgg tttaaacatc tccagcgccg ccttgccggt
    64141 catggcgaca tcaacgctgt tacctaattt ttccagcaca gaacgcgcaa caatcacgtt
    64201 cagttcaatg tcttccacca gcagcacatt cagcgccggt aaaggcatat cgtcttcatc
    64261 aaacgcatca tcgacctctt ctgctaccga cggtgcgtgg atcgtcaacg taaaggttga
    64321 acctttgccc tgttcgctgg taaccgtaat atcgccgccc atatttttcg ccagacgacg
    64381 agaaacggcc agaccaatac cggtgccggt ggcaggttta ccgccatgac tgtctttcac
    64441 ctggtaatac atggcgaaaa ttttatccag ctcatcctgc ggaatgccga taccagagtc
    64501 ttccacttca aaatgcagca tatcgccttc atcgtagcgc acgcgcacgg taacctggcc
    64561 ttgctgggtg aatttgacgg cgttactgat gaggttccac aggatctgcc gtaaacgcgt
    64621 cccgtcggta atgacctgat gcggtaatgg cagcgtcggc tccaggttaa agcgcaatcc
    64681 tttttgttgc gcctgcaatg cggagagatt ttccagatcg gcaaggaagc tggtgaaatc
    64741 aaccggttga ttatcaagct ggaccttgcg ccgttccatc ttatccatgt cgataatatc
    64801 gttaaagata ttccccagcg tgacggccga aacatggatg gtcttgagat atttttcctg
    64861 ctcggcggtg agttcggtat ccagcagaat acggctcagg ccgacgatac cgttcagcgg
    64921 tgtacgcaat tcgtgactga tggtggagat aaacgtcgtt ttgtcgcggc tggcccgttc
    64981 aagcgcatcc tgataccgct tacgctcggt aatgtcgcga ccaaagccca tcaaaccgtg
    65041 acgtttaccc acgcggtcgt agtacggcac tttacggatt tcaaagcagg ctttgcgccc
    65101 gtccgggtaa tccagccact gttcataggt cagtgacaca ttatgacgga acactttttc
    65161 atcggtttca atgacttttg cggcggcttc cggtgagtaa acatcagcag gtttcaggtg
    65221 aaccagttgt ttttcgcttt ttccggtcag cagctccatc gcgcggttac agccggaaaa
    65281 ctctttatct tcgttacgat aaaaaaccag gtcgggtgaa gcatcaagga aggaacgtaa
    65341 gaatgaggat tgctgctcga gctgaatttg tgtctcttcg cgctctttga tttcaatttt
    65401 cagttggccg aaggtttcct gcagttctgc ttccgctttt tcacgaacgg cgatttcctg
    65461 atttagctgg gcaatattat cttttaactg aacgttgagg ctcaaatcgc gctcgcgcat
    65521 ctcctccagt ttttgcacca gccgtgacag acgttgtcgt gactcctcca gttgctcgac
    65581 gaccaccgat agaaagtaga ccgcccacgg cgtaatcagc aaaccaaaga agatagaacg
    65641 aataacatca atgctttcga cctgaccatg cagcaccatg gttaccgcca tttgtaccac
    65701 aatggcaaga acgacgaggg ccagcgccag caacattgag aagcgcacca gacctaactt
    65761 catcatcagg tcaacataat actgcgccag cagacgaatt tgcttcatta gggaattcct
    65821 tcacgacaac ctgccccaat aatacccaat tctgacagtt acgttataaa ttgtgtgaga
    65881 aatgcggaat tcccctcaac gacctactcc gttggaggta tccacggacg ttccagcgcc
    65941 gatccctgta cgccatgttc attcagatag cgatccagct cgaccattcc cgtccatcga
    66001 ttttcgcacc acagaggagc aagcagcgtc gggcgacggg cgctggcgga aatgcggtgg
    66061 tagatcactt ccggtggcgt atggcgaatc atttctccgg cagtgagcgt gtaatcctcc
    66121 agttcaatac cgttcaaacg ccccgcttcc caggctttcg ccataatgct gcctttcaca
    66181 atatgcagcg gatgcagctt tatgccgtcc acgccggttt caaccactcg ctccagcgtt
    66241 tgcaaacatt cagcctgccc ttcgccaggc aggccgacaa ttaagtggga acacaccttc
    66301 agcccgcgct gacgtgccag ctgggttgta cgctgataac aggcaaaatc atggccgcgg
    66361 ttgatgcgat gcagtgtttt gtcgtgggcg gtttgtagcc ccagctccag ccacacttcg
    66421 taaccctggt ccttatattc gcaaagcaga tccagcaccg catccggcac gcagtccggg
    66481 cgggtaccaa cacacaaacc gacaatattg gcctggctca ccgcctgctg atacatagaa
    66541 cgcagcacct gaacttccgc aaaggtgctg gtatacgcct gaaagtaggc cagatagcgt
    66601 ttagcgcggt taactaaatt cgcctggtgc gccagttgct cggcaatgga acgatgctgc
    66661 tgcgcttcat cggcaaacga ggcaacatta cagaatgtgc agccaccacg cccgatggta
    66721 ccgtcacggt tagggcagct aaaaccgccg tgtagcgtca gcttatgcac cttttgccca
    66781 taacgacggg tgagatcacc accaaacata ttgactaatt tctgtaactg cataatctga
    66841 tagaccgcgc cttgaaaaga ggccaaagcc tgccattttt agctcaattc ggcgatgacc
    66901 tggatcaatc gtcccggccc gcttttatca actgcataat caatcaaaat taccgaaatt
    66961 tcatgcataa tcacataaat cacttttgct tatcttgtgt cagatttttt tatctcctta
    67021 tggattttag gcaaaaacag tagcatgaaa cgtcattacc aattaaggca gtataaaatg
    67081 ctggttttct cgtcagttca aggcaggata agggttaaca cgcctttatg acagtcagga
    67141 attgactgtt tctctaacga cttccctttt agccttaaag ataaaatcca ttttaatttc
    67201 agtaatttaa taaagaattt tgcgctaaag cacatttctg taccaataag cttgccattt
    67261 gacctgtatc agctttcccg ataagttgga aatccgctgg aagctttctg gatgagcagc
    67321 ctgctcatca tatttatgca gtaattgaga tcccctcttc accgtattaa ccgatgcgaa
    67381 aaggacaaca agggggcgaa tgcgaggcgc gcgtatgaca cgcaaacccc gtcgccacgc
    67441 tctttctgtg cccgtgcgca gcggttcgga agtggggttc ccgcagagcc tgggggaggt
    67501 tcacgatatg ttgtacgata aatcccttga gagggataac tgtggtttcg gcctgatcgc
    67561 ccacatagaa ggcgaaccta gccacaaggt agtgcgtact gcaatacacg cactggcccg
    67621 catgcagcac cgtggcgcga ttctcgccga tggtaaaacc ggcgacggtt gcggcttgct
    67681 gttacaaaaa ccggatcgct tttttcgcat cgttgcgcag gagcgcggct ggcgtttagc
    67741 aaaaaactac gctgtcggga tgctcttcct gaataaagat cctgaactcg ccgctgccgc
    67801 acgccgcatc gttgaagaag agctgcaacg cgaaaccttg tcgattgtgg gctggcgtga
    67861 tgtccccact aacgaaggcg tgctgggtga aatcgccctc tcctctctgc cccacattga
    67921 gcaaattttc gttaacgctc cggcaggctg gcgtccgcgc gatatggagc gccgtctgtt
    67981 tatcgcccgc cgccgcattg aaaagcgtct cgaagccgac aaagacttct acgtctgtag
    68041 cctgtcgaat ctggtgaaca tctataaagg tctgtgtatg ccggcggatc tgccgcgctt
    68101 ttatctggat cttgcggacc tgcgtctgga atcggccatc tgcctgttcc accagcgctt
    68161 ctccactaac accgtaccgc gctggccgct ggcgcagccg ttccgctacc tggcgcataa
    68221 cggtgaaatc aacaccatca ccggtaaccg ccaatgggcg cgtgcgcgta cctataaatt
    68281 ccagacgccg ctgatccctg atctgcacga cgccgcaccg ttcgtcaacg aaaccggttc
    68341 tgactccagt tcgatggata acatgctgga actgctgctg gcaggcggga tggatatcat
    68401 ccgcgccatg cgtctattag taccacccgc ctggcagaac aacccggata tggacccgga
    68461 actgcgtgcc ttctttgact ttaactccat gcatatggag ccgtgggatg gcccggcggg
    68521 catcgtgatg tccgatggtc gcttcgctgc ctgtaacctc gaccgtaacg gtctgcgtcc
    68581 ggcacgctac gtcattacca aagacaagct catcacctgc gcctctgaag tcggtatctg
    68641 ggattatcag cctgacgaag tggtcgaaaa aggccgcgtc gggccaggcg aactgatggt
    68701 gatcgacacc cgcagtgggc gtattctgca ctcggcagaa accgatgacg atctgaaaag
    68761 ccgccatcca tataaagagt ggatggagaa aaacgtccgc cgactggtac cgtttgaaga
    68821 tctgcccgat gaagaagtgg gtagccgcga actggacgac gacacgcttg ccagctacca
    68881 gaaacagttt aactacagcg cggaagagct ggactccgta attcgcgtac tgggcgaaaa
    68941 cggtcaggaa gcggtcggtt cgatgggcga tgatacccca ttcgccgtgc tctccagtca
    69001 gccgcgcatt atttacgact acttccgcca gcagtttgcc caggtgacta acccgccaat
    69061 cgacccgctg cgtgaagcgc atgttatgtc gctcgccacc agtatcggtc gtgaaatgaa
    69121 cgtcttttgc gaagcagagg gccaggcgca ccgtttaagc tttaaatcgc cgattctgct
    69181 ctactccgat ttcaaacagc tcacgacgat gaaagaggag cactaccgcg cagatacgct
    69241 ggatatcacc tttgacgtca ctaaaaccac gctcgaagcg acagtcaaag agctgtgcga
    69301 caaagccgaa aaaatggtac gtagcggcac cgtgctgctg gtgctctccg accggaatat
    69361 cgctaaagat cgcctgccgg ttccagcccc gatggcggtt ggcgcgatcc agacccgtct
    69421 ggtcgatcaa agcctgcgtt gcgatgccaa catcatcgtc gaaaccgcca gcgcccgcga
    69481 tccgcaccac ttcgccgtgt tgctgggctt cggcgcgacg gctatttatc cataccttgc
    69541 ctatgaaacg ctgggccgcc tggtagacac ccatgcgatt gccaaagatt atcgtaccgt
    69601 gatgctcaac taccgtaacg gcatcaacaa aggcttgtac aaaatcatgt ccaaaatggg
    69661 catctccacc atcgcctctt accgctgctc gaaactgttt gaagcggtcg gtctgcacga
    69721 tgatgtagtg ggcctgtgct tccagggggc ggtcagccgc attggtgggg cgagctttga
    69781 agacttccag caggatctgc tgaatctgtc gaaacgtgcc tggctggcgc gcaagcccat
    69841 cagccagggc ggcctgctga aatacgtcca cggcggcgaa taccacgcct acaacccgga
    69901 cgtggtgcgc acgctgcaac aggcggtaca gagcggcgag tatagcgact atcaggaata
    69961 cgcgaagctg gttaatgagc gtccggcaac cacgctgcgc gatctgctgg caattacgcc
    70021 gggtgaaaac gcggtcaaca ttgctgatgt tgaaccggca agcgaactgt ttaaacgctt
    70081 tgataccgcc gcgatgtcta tcggcgcgtt aagcccggaa gcccacgagg cgctggcgga
    70141 agcgatgaat agcatcggcg gtaactcgaa ctccggtgaa ggcggcgaag acccggcgcg
    70201 ctatggcact aacaaagtgt cgcgcatcaa gcaggtggct tctggtcgct tcggcgttac
    70261 tccggcgtat ctggtcaatg ccgacgtcat tcagattaaa gtcgcccagg gcgcgaagcc
    70321 gggcgaaggc ggtcagttgc cgggcgacaa agtcactcct tacatcgcca aactgcgcta
    70381 ttcggtgccc ggagtgacgc tgatctcccc gccgccgcac cacgatatct actctatcga
    70441 ggacttagcg cagctcattt tcgacctcaa gcaggttaac ccgaaagcga tgatctccgt
    70501 gaagctggtt tccgaaccgg gcgtaggcac catcgcgact ggcgtggcaa aagcttatgc
    70561 cgatttaatc accatcgcag gctatgacgg tggcaccggc gcaagcccgc tgtcgtcagt
    70621 gaaatacgca ggttgcccgt gggagctggg gcttgttgaa acccagcagg cgctggttgc
    70681 caatggtctg cgtcataaga tccgtttgca ggtcgatggc ggcctgaaaa ccggtgttga
    70741 tatcatcaaa gcggcgattc tcggcgcaga aagcttcggc ttcggcactg gcccgatggt
    70801 ggcgctcggc tgtaaatatc tacgtatttg ccatctgaac aactgcgcaa cgggtgtagc
    70861 aactcaggat gacaaactgc gtaagaacca ctatcacggc ctgccgttca aggttaccaa
    70921 ttactttgaa tttattgccc gtgaaacccg cgagctgatg gcacagctgg gcgtaacgcg
    70981 tctggtggat ctgattggtc gcaccgacct gctgaaagaa ctggacggtt tcaccgccaa
    71041 acagcaaaaa ctggcgctgt cgaagctgct ggagaccgcc gaaccgcatc caggtaaggc
    71101 actctactgc accgaaaaca acccaccgtt tgataacggc ctgctgaacg cgcagttgct
    71161 gcaacaggcg aaaccgtttg tcgatgaacg ccagagcaaa accttctggt tcgatattcg
    71221 caacaccgac cgttctgtcg gcgcgtcgct ttcaggctat atcgcccaga cgcacggcga
    71281 tcaggggctg gcagccgatc ctatcaaagc gtacttcaac ggcaccgcag gccagagttt
    71341 cggtgtgtgg aacgcgggcg gcgtggaact gtacctgacc ggggatgcca acgactatgt
    71401 cggtaaaggc atggcgggcg gcttaatcgc cattcgtcct ccggttggtt ccgccttccg
    71461 cagccatgaa gcaagtatta tcggcaacac ctgcctgtat ggcgcgaccg gtggtcgtct
    71521 gtatgccgca ggccgcgcgg gtgaacgttt cggcgtgcgg aactccggtg ctatcaccgt
    71581 ggtagaaggc attggcgaca acggctgtga atatatgacg ggcggtattg tctgtattct
    71641 gggtaaaacc ggcgttaact tcggtgcggg catgaccggc ggcttcgctt acgttctcga
    71701 tgaaagcggt gatttccgca aacgcgttaa cccggaactg gtcgaggtct taagcgttga
    71761 cgatctggcg atccatgaag agcatctgcg cggtcttatc accgagcatg ttcagcatac
    71821 tggctctcag cgcggtgaag agattctggc gaactggtca accttcgcca ctaaatttgc
    71881 gctggttaaa ccgaagtcca gtgatgtaaa agcactgctg ggtcatcgta gtcgtagcgc
    71941 agcagagttg cgcgtgcagg cgcagtaagg ggtagcaaca atgagtcaga atgtttatca
    72001 atttatcgac ctgcagcgcg ttgatccgcc aaagaagccg ctgaagatcc gcaaaattga
    72061 gtttgttgaa atttacgagc cgttttccga aggccaggcc aaagcgcagg ctgaccgctg
    72121 cctgtcgtgc ggcaacccat actgcgagtg gaaatgtccg gtacacaact acatcccgaa
    72181 ctggctgaag ctcgccaacg aggggcgtat ttttgaagcg gcggaactgt cgcaccagac
    72241 caacaccctg ccggaagttt gcggacgagt ctgcccgcaa gaccgtctgt gcgaaggttc
    72301 ctgcactctg aacgatgagt ttggcgcggt gaccatcggc aacattgagc gttatatcaa
    72361 tgataaagcg ttcgaaatgg gctggcgtcc ggatatgtcc ggcgtgaagc aaaccggtaa
    72421 aaaagtggcg attatcggcg caggcccggc aggtctggcg tgtgcggatg tcctgacgcg
    72481 taacggcgta aaagccgttg tcttcgaccg tcatccagaa attggcgggc tgctgacctt
    72541 cggtattccg gccttcaagc tggaaaaaga ggtaatgacg cgtcgccgtg aaatcttcac
    72601 cggcatgggt attgaattca aactcaatac cgaagtgggc cgcgacgtgc agctggacga
    72661 tctgctgagt gattacgatg ccgtcttcct tggcgtcggg acttatcagt caatgcgcgg
    72721 cgggctggaa aacgaagacg ccgatggcgt gtacgcagcg ctgccgttcc ttatcgccaa
    72781 caccaaacag ttaatgggct ttggtgaaac ccgcgacgaa ccgttcgtca gcatggaagg
    72841 caaacgcgtg gtggtccttg gcggtggcga cactgcgatg gactgcgtgc gtacgtccgt
    72901 gcgccaggga gcgaagcacg ttacctgtgc ctatcgtcgt gatgaagaga acatgccggg
    72961 ttcccgccgc gaagtgaaaa acgcgcggga agaaggcgta gagttcaaat tcaacgtcca
    73021 gccactgggg attgaagtga acggtaacgg taaagtcagc ggcgtaaaaa tggtacgtac
    73081 cgaaatgggc gaaccggatg ccaaaggccg tcgccgcgcg gagatcgttg caggttccga
    73141 acatatcgtt ccggcagatg cagtgatcat ggcgtttggt ttccgtccac acaacatgga
    73201 atggctggca aaacacagcg tcgagctgga ttcacaaggc cgcatcatcg ccccggaagg
    73261 cagcgacaac gccttccaga ccagcaaccc gaaaatcttt gctggcggcg atatcgtccg
    73321 tggttccgat ctggtggtaa ccgctattgc cgaaggtcgt aaggcggcag acggtattat
    73381 gaactggctg gaagtttaag ctctgtccca gcgccgcctc ctcagaaaag agtcggcgct
    73441 gaaattctcc tttctttctg gaaagcgcct cgcataatct gaagaaaacc aatgtaatac
    73501 gctcatctgg attgttaccg gtagatgccc ggcgtatagc tgattccatt attcaacgca
    73561 gcgccatata aagggacaga taatggaatc gctctcagag agaacctcaa caggctacca
    73621 gcaaatccac gacggtatta ttcatctggt cgatagcgcc cggacggaaa cggtacgtag
    73681 cgttaacgcg ctgatgaccg cgacatactg ggaaattggc cgacgaattg tcgaatttga
    73741 acaaggtggc gaggccaggg ctgcgtatgg tgcgcagcta atcaagcgac tatcaaagga
    73801 tttaagtcta aggtataagc gtgggttctc tgcaaaaaac ttacgccaaa tgaggctttt
    73861 ttacctcttt tttcaacatg ttgaaattcg ccagacagtg tctggcgaat taacaccatt
    73921 gggaattccg cagacaccat ctgcagaatt cccatcggca aagatttggc agacgctgtc
    73981 tgccaaatct ttccccctgc cctggtccac ctacgtccgt ttactttccg ttaaaaacgc
    74041 tgacgcccgc agcttttatg aaaaagagac gctccgctgt ggctggtctg ttcgccagct
    74101 agaacggcaa attgcgaccc aattttatga gcgaacacta ctgtcacatg acaaatcagc
    74161 catgctgcaa caacacgctc ctaccgagac gcatattctt ccgcaacagg cgatacgcga
    74221 tccctttgtg ctcgaatttc tggaattgaa agatgaatat tcagaatctg atttcgagga
    74281 ggcgctgatc aaccacctga tggatttcat gctggaactt ggggatgatt ttgcctttgt
    74341 tggtcggcag cgaaggttac gcattgatga caactggttt cgtgtcgatc tgctgttttt
    74401 ccaccgccgt ttacgctgcc tactaatcgt cgatctaaaa gtgggcaaat tcagctatag
    74461 cgatgccgga cagatgaata tgtatctcaa ctacgccaaa gagcactgga cgctaccgga
    74521 tgaaaatccg cccatcggtc tggttctctg tgcagagaaa ggagccggag aagcacatta
    74581 tgctctggca ggtttgccta acaccgttct ggcaagcgaa tataagatgc aactacctga
    74641 tgagaaacga ctcgcagatg aactcgttcg aacacaggcg gtgctagagg aaggctatag
    74701 acgccgttaa tttcagcggt ttcgaattct gcagacagtg tctgcagaat ctttaaaacg
    74761 aactaagttt taagccatta aatcagcctt aaccttcacc acaacctttt taatctctcc
    74821 aggctcgccg acaacgcatc ctggtttatg cggttcacct ggcataaaca cggcgaacat
    74881 tcccggtttt aagatgatgg cttgctcgtt atcaatggtg ctgcaaagct gataatcatc
    74941 ctcatggtgg aactcttcac actggcgcgc agtgcctgcc atgccaaaca gaatccgttc
    75001 ctcaccgttt aataacagtt ggatatcaat gtattgctcg tgcaattccg cttttttctc
    75061 gacgggcgat tgagtgttaa acgtcatgac attcataaag atattgtcgc cctgtaattc
    75121 gtaacgaccc ggcgcttttt cttgcggtct ggcagctaat gccagcgtta acgcgtcctg
    75181 taacgcagga tgtaacccag cagacggtaa tgactgtact tcacccatca tcataatttt
    75241 tctccctggg ccaacagcgc agccccaagt aaacctgcat cgtggcggta gtgcgccgcc
    75301 agtaaatcaa catgaaatgc cgctggctcc tgcgccagat acgtttccac cagcgccaga
    75361 tacccttctg ccagcccaac gctgccaccg accaccacgc actggcaatc agttgtggct
    75421 ttaatatcag cgatcagcct tgcaagcgta cgtgcggagc ggtgaatcag ctgctgcgcc
    75481 tgctcgtcgc cctgcccggc gcgtgtgaaa atagttctcg catccgcgcc cgccagttca
    75541 ccctgcgctg ccgccgcaat gccgcgacca gaagcaatcg cctccacgca acctgtgcgt
    75601 ccacagccac agaccgggcc gtgtggatcg gcaagcgtat gcccgatatg ccccgccaga
    75661 ccgccagggc cggtaagcag tttgccgccg ctcactacac cgccgccgac gccagtggaa
    75721 acggtgataa acaccatctc ggttacatcg ccttccagcg cctgatactc cgcccatgct
    75781 gcagcctgcg cgtcgttaat ggcaatggtc ggcaaattgg taagttgttc cagcgttttg
    75841 actaacggaa agtgtagcaa tccaccgaga ttatgcggat tcagcgccag caaactgccg
    75901 tcgcgaataa ttccggttga agcgatggca acccgctgcg catgagcttg caacggagag
    75961 actaatgcgg ataaggcatc acgcaaggct tctggtgtct ggctggctgg cgtaggaagt
    76021 tcacgacgat cgcggatctg cccgtcagcg ccaatcagcg cggcggcaag tttagtaccg
    76081 ccgatatcaa tcgccagcgt ggtcatagca ccgccttttt catcgctgtg ttgtaccact
    76141 gacaaatgtg ttcaagacgc gtaatggcag aaccgaccgt caccgcccac gcgccgtggc
    76201 gcatcgcatc tgccgcctga gcaggcgtgt tgtaacgccc ttcggcaatc accctacatc
    76261 cggcgtcgct caacgttttc accagcgcca gatccggctc ttctggcgtt tcaggcgtgg
    76321 tatagccaga aagcgtagtg ccaataattt cggctcccag cttttggcat gccaggccgt
    76381 cttccggcgt tgagcagtcg gtcatcgcca gtaaaccgtg atggtgaata cgtgccagca
    76441 gcgtttcaac aggcaccgga cgcgggcggt cggtgccgtc aatggcgata atgtccgcgc
    76501 ccgcctgcgc cagcgcatca acatcttcaa tataggccgt gatgcgtacc ggagaatcct
    76561 ccagatcgcg tttcacaatt ccaataatcg gcacgctcac caccgcacgc gtggcttgca
    76621 gatttgccac accttcaatg cgaatggcaa ccgcgcccgc ctgttctgcc gctaatgcca
    76681 tggcggcgac gatttcgggt ttatcgagcg ggctgtccgg aaccggctgg caggagacaa
    76741 tcaggccacc gttagcagcg attttttgat ccagttgtgc aagtaacgac atacatcttc
    76801 ccttagcgaa aggcccggta catagaccgg gcaacaggat taacttttgg ttttgactaa
    76861 atcgtttttg gcgctgccaa atggcacggc accgctaaat ggtttgccgt cgatagcgtc
    76921 atgagtacgc aatgcttccg ggcgcagcca acgctgaacg cgggaaggca tatccagacc
    76981 aatcagcagg atcaccacaa atgtcagact gaacgatagc gatgccagcg cagtacccag
    77041 atccagacgt tgagcgatca acgcgccgat gattggggcc agtgcgccgc ccaatgcgcc
    77101 aacgttgtag gtaaagccca ggcccgctgc acgctggtcg gtatcgaaat aaccgccaat
    77161 cagtttcggt aagatcccgg cgatcccttg tccaagcatt tgctggaaga acagtaacag
    77221 accgagcacc cagacgtttg cgccgccaat cgcaaatacc ggaataatca acagctgcga
    77281 ggccaacagg ctacaaacgt aggctttgcg ggttcccagc cagtcaccga ggaagccgcc
    77341 gacacagcac cccaccgcag ccccaaagcc gctaaagaac agcacattgg cgacagtatg
    77401 cgggtcataa gccagatcgg ttttcagata cgttggcagc agcgcctgaa tcggccatga
    77461 gtagaggaaa gcaaacaaca cgaccaccat cagcattacg cccgttggcc agcgtttgcc
    77521 cgtactctgc accataaagc tgataaagat tgcggcgcat aacagcccaa gaacagcgac
    77581 gatcgcggca ttttgcaggt taccggcgaa gcagaaccac agcgcagtag ccgccgccag
    77641 tgtcattacg atattggcaa tgcgatgttc accacggtag agaatatcca ccattgtgcg
    77701 taccggtgct ttacccccgt gtttctcttt ccagtcttcc gcttccggga tgtttttacg
    77761 cagccagaga gcaaagatga ttggcaaaat gccgataaag aacagcgcac gccagcccca
    77821 gaccggaacc accaggctat agacctgagc ggcaacgacg gcccccacag agaagcctga
    77881 aatcagaaaa ccactggctt tgttacgcag atgttttggc cagctttcaa tgacataggt
    77941 ggcgctggaa ccgtattcac ccgccatccc catgccgatg accagacgcg cgataaacat
    78001 ggtgatgtag cctggcgcaa agccgcaggc cagcgtcccg gccgagaaga gaacgatgct
    78061 ggtgaccatt gccagacgac gcccgtagcg gtcacccata gcgccgagca tcaggccgcc
    78121 gaaccagcga gagataaagg ctgcggagat cagacttgcc gcctgcaccg tcgtcagccc
    78181 gaattcaccc tgtacttcgg tgagtaccag ggcgattaaa acgaaatcaa aaccgtcaag
    78241 cagatatccc aaccaggcag cggaaaatgc gcgccattgt gcacggttga gatggcgata
    78301 ccacgggata ttctgggttg tagtactcat tgtgagtctc ccgcggtggg cgatgcccac
    78361 acgctttggt ataaaaattg tagggtacag atgcgtttat ttcccctcac ccggtagggg
    78421 cgagcgaggg gaaacaactc acccgcgctc ttgcatcaac tgctgggcca gcgccttcag
    78481 ttctggcaga tatttttcat ctaccggtcc aaacggtttg cggcacagcg gcacagaaac
    78541 gacatccata taatggagga cagttttcag gccgcggaat acgcccgttt tgatcagtaa
    78601 atcaatgact ttattgcatt cagtttgcag tttctgcgcg gtctggatat cgccttcttt
    78661 cagcgcctta acgatcccct gatagcgcca gcccatgatg ttgtaggtac taccgatacc
    78721 accatcagcg cccgccagca gaccagaggc gaagatttcg tcgtaaccgt tatagagcac
    78781 aagatcagga tgttcacgac ggatctgctc catctgatag agatcgccag aggtctgttt
    78841 cagcgcacct acgccaggca atgtaacaag tgtgttgatc tgatccaggg tcagttttac
    78901 cccactcagg gctggaatgt tgtacaccac catcggcaaa ccatccgccg aatcaataat
    78961 tgcccgatag tgatcgcagt gttcttcaaa gctgaaagga tagtagaacg gcgtgacggc
    79021 ggagacggca tcgaagccat aacgtttagc cgatgccgca agttgttggc tttcggcggt
    79081 gctgacgcaa ccgacgtggg cgatgagttt aatcttacct ttcgcctctt cggcgacgat
    79141 ttccagtacc tgttcacgct cggaaaggct ttgtacaaag gcctcgccgg tcgaaccacc
    79201 cacgtataaa ccgtcgatgc cctgctgaat attgaactga accaggcgac gcagactcgc
    79261 tttatccagt gcttgttgtt gatcaaaagg agtcaggagt gcagccatta cgccacgtaa
    79321 attcgttgcc ataaatacct ctgaagtgat gcttgtctga taaacgatat acctttatac
    79381 ctgttatacc agatcaatta agcaacaccc catacagaaa gcttataatg cgatctgctt
    79441 cactaaagtg gcattatttc tttttgttgg tggtctgacc gaaagcgtgc caggtagcag
    79501 agacgctgtt gagatgcgat tgcaacgcac gatcggcttc gtcaggatca tgacggcgga
    79561 tcgcatcaac gatcgcaata tgctgttgat aactaacgtt gttatgttcg tgcagtgcct
    79621 gatcggtaac cgttgggcgt gcggcaataa gccagtcgag cagggcaacg tggatcgcca
    79681 tgaagattgg gttaccgggg atctccgcca gcacgcggtg gaaatcaacg tctgaacgaa
    79741 tgaatgccgc gttgttatcc agcgactgac tgttaatttc cagtgctttt gccagcaaat
    79801 cgatttgctc atcggtggca tgttcagccg catagcgcac caggctggat tcaaagaaca
    79861 gacgtaattg ttcgaaatgg gcaatcccac caggatgaga aaggaaatct ttcgccatgc
    79921 cggaaagctc gccgatgatg gtgtccgcag aaggacgtga gacgcgggca cgttcgccgt
    79981 tgttgatttg caccagacct ttgcgtttta acgctgccag cgcttcgcgc acagaaggac
    80041 gcccgacgtt aaagaacgcc atcagttcgc gttcagacgg taattgttca ccttcgccaa
    80101 attcacgacg gcggatcatc tgttccagct cttcttccac catttcggag agttttttac
    80161 gcgccagcgg gcggctacgc aagttgcgac caattgcagg tgaagaatct tcggtttgcg
    80221 aatcaaatgc gttcataagg cccattctgt aaggtcagtg tgattaacat catcagtgac
    80281 atcctatcac aggattgaaa gtaggggaaa atggcaggtt tttctctttg tgcctcatca
    80341 ttaccataat taacggaata attaactatt gcgaaaaatt aatgtaacgc agataaaaac
    80401 atcccgtttg aattatttat aag
//
LOCUS       DBANUU010000003        69392 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000003 DBANUU010000000
VERSION     DBANUU010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.003"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..505
                     /locus_tag="ACIE68_000162"
     CDS             <1..505
                     /locus_tag="ACIE68_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFX5906539.1"
                     /translation="KCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQETKTVN
                     IISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGGAVG
                     AAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRIQPN
                     TKCPEKS"
     gene            521..1060
                     /locus_tag="ACIE68_000163"
     CDS             521..1060
                     /locus_tag="ACIE68_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFX5906540.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1155..2732)
                     /gene="guaA"
                     /locus_tag="ACIE68_000164"
     CDS             complement(1155..2732)
                     /gene="guaA"
                     /locus_tag="ACIE68_000164"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFX5906541.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2801..4267)
                     /gene="guaB"
                     /locus_tag="ACIE68_000165"
     CDS             complement(2801..4267)
                     /gene="guaB"
                     /locus_tag="ACIE68_000165"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFX5906542.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4429..5799
                     /gene="xseA"
                     /locus_tag="ACIE68_000166"
     CDS             4429..5799
                     /gene="xseA"
                     /locus_tag="ACIE68_000166"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFX5906543.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5796..6011)
                     /locus_tag="ACIE68_000167"
     CDS             complement(5796..6011)
                     /locus_tag="ACIE68_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFX5906544.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6080..7552)
                     /gene="der"
                     /locus_tag="ACIE68_000168"
     CDS             complement(6080..7552)
                     /gene="der"
                     /locus_tag="ACIE68_000168"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFX5906545.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDACAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7670..8848)
                     /gene="bamB"
                     /locus_tag="ACIE68_000169"
     CDS             complement(7670..8848)
                     /gene="bamB"
                     /locus_tag="ACIE68_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFX5906546.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8859..9479)
                     /gene="yfgM"
                     /locus_tag="ACIE68_000170"
     CDS             complement(8859..9479)
                     /gene="yfgM"
                     /locus_tag="ACIE68_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFX5906547.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9497..10771)
                     /gene="hisS"
                     /locus_tag="ACIE68_000171"
     CDS             complement(9497..10771)
                     /gene="hisS"
                     /locus_tag="ACIE68_000171"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFX5906548.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10882..12000)
                     /gene="ispG"
                     /locus_tag="ACIE68_000172"
                     /gene_synonym="gcpE"
     CDS             complement(10882..12000)
                     /gene="ispG"
                     /locus_tag="ACIE68_000172"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFX5906549.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12027..13040)
                     /gene="rodZ"
                     /locus_tag="ACIE68_000173"
     CDS             complement(12027..13040)
                     /gene="rodZ"
                     /locus_tag="ACIE68_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFX5906550.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13325..14479)
                     /locus_tag="ACIE68_000174"
     CDS             complement(13325..14479)
                     /locus_tag="ACIE68_000174"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFX5906551.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14629..15060)
                     /gene="ndk"
                     /locus_tag="ACIE68_000175"
     CDS             complement(14629..15060)
                     /gene="ndk"
                     /locus_tag="ACIE68_000175"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFX5906552.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15209..17521)
                     /gene="pbpC"
                     /locus_tag="ACIE68_000176"
     CDS             complement(15209..17521)
                     /gene="pbpC"
                     /locus_tag="ACIE68_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFX5906553.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDHWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAKLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17522..22483)
                     /gene="yfhM"
                     /locus_tag="ACIE68_000177"
     CDS             complement(17522..22483)
                     /gene="yfhM"
                     /locus_tag="ACIE68_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFX5906554.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGLTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAGFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPDETVADQQKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22690..23535
                     /gene="sseA"
                     /locus_tag="ACIE68_000178"
     CDS             22690..23535
                     /gene="sseA"
                     /locus_tag="ACIE68_000178"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFX5906555.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDACMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQVIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23871..23996
                     /gene="timP"
                     /locus_tag="ACIE68_000179"
     CDS             23871..23996
                     /gene="timP"
                     /locus_tag="ACIE68_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFX5906556.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNRNLSGDNLLVINNLQSSR"
     gene            complement(24028..24804)
                     /gene="sseB"
                     /locus_tag="ACIE68_000180"
     CDS             complement(24028..24804)
                     /gene="sseB"
                     /locus_tag="ACIE68_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFX5906557.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQVAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(24946..26229)
                     /gene="pepB"
                     /locus_tag="ACIE68_000181"
     CDS             complement(24946..26229)
                     /gene="pepB"
                     /locus_tag="ACIE68_000181"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFX5906558.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26288..26488)
                     /gene="iscX"
                     /locus_tag="ACIE68_000182"
     CDS             complement(26288..26488)
                     /gene="iscX"
                     /locus_tag="ACIE68_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFX5906559.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26500..26835)
                     /gene="fdx"
                     /locus_tag="ACIE68_000183"
     CDS             complement(26500..26835)
                     /gene="fdx"
                     /locus_tag="ACIE68_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFX5906560.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(26837..28687)
                     /gene="hscA"
                     /locus_tag="ACIE68_000184"
     CDS             complement(26837..28687)
                     /gene="hscA"
                     /locus_tag="ACIE68_000184"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFX5906561.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALVARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVMATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEASEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGNDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28704..29219)
                     /gene="hscB"
                     /locus_tag="ACIE68_000185"
     CDS             complement(28704..29219)
                     /gene="hscB"
                     /locus_tag="ACIE68_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFX5906562.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWAAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29315..29638)
                     /gene="iscA"
                     /locus_tag="ACIE68_000186"
     CDS             complement(29315..29638)
                     /gene="iscA"
                     /locus_tag="ACIE68_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFX5906563.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29655..30041)
                     /gene="iscU"
                     /locus_tag="ACIE68_000187"
     CDS             complement(29655..30041)
                     /gene="iscU"
                     /locus_tag="ACIE68_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFX5906564.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30069..31283)
                     /gene="iscS"
                     /locus_tag="ACIE68_000188"
     CDS             complement(30069..31283)
                     /gene="iscS"
                     /locus_tag="ACIE68_000188"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFX5906565.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31395..31883)
                     /gene="iscR"
                     /locus_tag="ACIE68_000189"
     CDS             complement(31395..31883)
                     /gene="iscR"
                     /locus_tag="ACIE68_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFX5906566.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(32153..32893)
                     /gene="trmJ"
                     /locus_tag="ACIE68_000190"
     CDS             complement(32153..32893)
                     /gene="trmJ"
                     /locus_tag="ACIE68_000190"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFX5906567.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            33012..33815
                     /gene="suhB"
                     /locus_tag="ACIE68_000191"
     CDS             33012..33815
                     /gene="suhB"
                     /locus_tag="ACIE68_000191"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFX5906568.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            33960..34814
                     /locus_tag="ACIE68_000192"
     CDS             33960..34814
                     /locus_tag="ACIE68_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFX5906569.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGRGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35005..36285
                     /gene="csiE"
                     /locus_tag="ACIE68_000193"
     CDS             35005..36285
                     /gene="csiE"
                     /locus_tag="ACIE68_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFX5906570.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36277..37416)
                     /locus_tag="ACIE68_000194"
     CDS             complement(36277..37416)
                     /locus_tag="ACIE68_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFX5906571.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37576..38466)
                     /gene="hcaR"
                     /locus_tag="ACIE68_000195"
     CDS             complement(37576..38466)
                     /gene="hcaR"
                     /locus_tag="ACIE68_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFX5906572.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38602..39963
                     /gene="hcaE"
                     /locus_tag="ACIE68_000196"
     CDS             38602..39963
                     /gene="hcaE"
                     /locus_tag="ACIE68_000196"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFX5906573.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            39960..40478
                     /gene="hcaF"
                     /locus_tag="ACIE68_000197"
     CDS             39960..40478
                     /gene="hcaF"
                     /locus_tag="ACIE68_000197"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFX5906574.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40478..40798
                     /locus_tag="ACIE68_000198"
     CDS             40478..40798
                     /locus_tag="ACIE68_000198"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFX5906575.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            40795..41607
                     /gene="hcaB"
                     /locus_tag="ACIE68_000199"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             40795..41607
                     /gene="hcaB"
                     /locus_tag="ACIE68_000199"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFX5906576.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41617..42819
                     /gene="hcaD"
                     /locus_tag="ACIE68_000200"
     CDS             41617..42819
                     /gene="hcaD"
                     /locus_tag="ACIE68_000200"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFX5906577.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            42916..43338
                     /locus_tag="ACIE68_000201"
     CDS             42916..43338
                     /locus_tag="ACIE68_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFX5906578.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43386..44258)
                     /locus_tag="ACIE68_000202"
     CDS             complement(43386..44258)
                     /locus_tag="ACIE68_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFX5906579.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44270..45364)
                     /locus_tag="ACIE68_000203"
     CDS             complement(44270..45364)
                     /locus_tag="ACIE68_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFX5906580.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45397..46395)
                     /locus_tag="ACIE68_000204"
     CDS             complement(45397..46395)
                     /locus_tag="ACIE68_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFX5906581.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46420..47931)
                     /locus_tag="ACIE68_000205"
     CDS             complement(46420..47931)
                     /locus_tag="ACIE68_000205"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFX5906582.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(47954..48937)
                     /locus_tag="ACIE68_000206"
     CDS             complement(47954..48937)
                     /locus_tag="ACIE68_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFX5906583.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAAEKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVLAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49034..52315)
                     /locus_tag="ACIE68_000207"
     CDS             complement(49034..52315)
                     /locus_tag="ACIE68_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFX5906584.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTPEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPERAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52433..53626
                     /locus_tag="ACIE68_000208"
     CDS             52433..53626
                     /locus_tag="ACIE68_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFX5906585.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWM
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(53699..54952)
                     /gene="glyA"
                     /locus_tag="ACIE68_000209"
     CDS             complement(53699..54952)
                     /gene="glyA"
                     /locus_tag="ACIE68_000209"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFX5906586.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55280..56470
                     /gene="hmpA"
                     /locus_tag="ACIE68_000210"
     CDS             55280..56470
                     /gene="hmpA"
                     /locus_tag="ACIE68_000210"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFX5906587.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKQEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56515..56853)
                     /gene="glnB"
                     /locus_tag="ACIE68_000211"
     CDS             complement(56515..56853)
                     /gene="glnB"
                     /locus_tag="ACIE68_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFX5906588.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(56914..58248)
                     /gene="glrR"
                     /locus_tag="ACIE68_000212"
     CDS             complement(56914..58248)
                     /gene="glrR"
                     /locus_tag="ACIE68_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFX5906589.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58238..58951)
                     /gene="qseG"
                     /locus_tag="ACIE68_000213"
     CDS             complement(58238..58951)
                     /gene="qseG"
                     /locus_tag="ACIE68_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFX5906590.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(59116..60543)
                     /gene="qseE"
                     /locus_tag="ACIE68_000214"
     CDS             complement(59116..60543)
                     /gene="qseE"
                     /locus_tag="ACIE68_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFX5906591.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61101..64988)
                     /gene="purL"
                     /locus_tag="ACIE68_000215"
                     /gene_synonym="purI"
     CDS             complement(61101..64988)
                     /gene="purL"
                     /locus_tag="ACIE68_000215"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFX5906592.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAIAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            65246..66802
                     /gene="mltF"
                     /locus_tag="ACIE68_000216"
     CDS             65246..66802
                     /gene="mltF"
                     /locus_tag="ACIE68_000216"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFX5906593.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(66799..67302)
                     /gene="tadA"
                     /locus_tag="ACIE68_000217"
     CDS             complement(66799..67302)
                     /gene="tadA"
                     /locus_tag="ACIE68_000217"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFX5906594.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(67360..67995)
                     /gene="yfhb"
                     /locus_tag="ACIE68_000218"
     CDS             complement(67360..67995)
                     /gene="yfhb"
                     /locus_tag="ACIE68_000218"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFX5906595.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            68204..69052
                     /locus_tag="ACIE68_000219"
     CDS             68204..69052
                     /locus_tag="ACIE68_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFX5906596.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            69108..69368
                     /gene="yfhL"
                     /locus_tag="ACIE68_000220"
     CDS             69108..69368
                     /gene="yfhL"
                     /locus_tag="ACIE68_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFX5906597.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
BASE COUNT        16449 a        19145 c        17510 g        16288 t
ORIGIN      
        1 aaaatgtctt ctgcctgtgg caatgttagc gtcattcact ctggcaggat gccagtcaaa
       61 tgctgacgat catgccgccg atgtttatca aaccgatcaa ctgaatacca aacaagaaac
      121 taaaaccgtt aatattattt ccattcttcc cgcaaaagtt gccgtagaca actcccaaaa
      181 taaacggaac gcacaagcct tcggcgcgct tattggcgca gtcgctggcg gtgttatcgg
      241 ccacaacgtc ggttctggca gcaattccgg aacgacggca ggggcagttg gcggcggagc
      301 tgtaggcgcg gcagcgggtt ctatggtgaa tgataaaacc ttagtggaag gtgtttcttt
      361 aacatataag gaaggcacca aagtgtatac ctctacccag gtgggtaaag agtgccagtt
      421 tacgacaggt ttagccgttg ttattaccac aacgtataac gaaacgcgta ttcagccaaa
      481 tactaaatgt cctgaaaaga gctaataatc aggaggagtc atgaagaaag tttttctttg
      541 cgccatcttg gcctccttaa gctatccggc tatcgcctca tcattgcagg atcaactctc
      601 tgctgtagca gaagctgaac agcaaggtaa aaatgaagag caaaggcagc atgacgaatg
      661 ggtcgcggag cgcaacaggg aaatccagca agagaagcaa cgtcgcgcaa atgcccaggc
      721 cgccgctaac aaaagagcgg caacggcagc agcaaataag aaagctcgtc aggataaact
      781 ggacgccgaa gcctctgcgg acaaaaaacg cgatcaaagt tatgaagatg agctacgcag
      841 cttagagatt cagaaacaaa aactggcgct ggcgaaagaa gaagcccgcg ttaagcgaga
      901 aaacgaattt atcgatcagg aactgaagca caaagctgcg caaaccgatg tggtgcaatc
      961 tgaagctgac gccaacagaa atatgactga aggcggtcgc gatctgatga aaagcgtggg
     1021 caaagcagaa gagaacaaat cggacagctg gtttaattaa tcgatgttag taacttcaag
     1081 cctatgattc ttgaagataa aaaaccctct gtagtaacag agggttttgt tcattcatag
     1141 tgcagggatt aaaatcattc ccactcaatg gtagctggcg gcttgccgct gatgtcatac
     1201 accacgcggg aaataccgtt cacttcattg ataatgcggt tggaaacgcg accgaggaaa
     1261 tcgtacggca gatgcgccca gtgtgcggtc ataaagtcga tggtttcgac agcacgcaga
     1321 gagacaaccc agtcatactt acgaccatcg cccattacgc caacggaacg taccggcagg
     1381 aacacagtga acgcctggct gactttgtcg tacaggtccg ctttacgcag ttcttcaatg
     1441 aagatggcgt cagcacggcg cagcaggtca cagtactctt tcttcacttc acccagcaca
     1501 cgaacgccaa ggcctggtcc cgggaacggg tgacggtaca gcatgtcgta cggcaggccc
     1561 agctccagac caatcttacg cacttcgtct ttgaacagct ctttcagcgg ttcaaccagg
     1621 cccatcttca tctctttcgg caggccgccc acgttgtggt gagatttgat gacgtgtgct
     1681 ttaccggttg cagaagccgc agattcgata acgtcagggt agatggtgcc ctgcgccagc
     1741 cacttcacgt cttccagttt cagcgcttct tcatcgaata cttcaacgaa aacgcgcccg
     1801 atgattttac gttttgcttc cggatcgttt tcgccagcca gcgctgacag gaagcgatct
     1861 tctgccggta cgtgaacaat gttaagacca aagtgatcgc caaacatatc cagaacctgc
     1921 tctgcttcgt tgagacgcag caggccgttg tcgacgaata cgcaagtcag gtttttaccg
     1981 atagcgcggt gcagcagcat tgcggttacg gaggaatcca caccaccaga gaggccgagg
     2041 atgactttat cgtcgccgac ctgctcgcgg atgcgagcta cagcatcgtc gataattttc
     2101 gctggcgtcc acagggcttc acactggcag atatcacgca caaaacgctc cagcatgcgc
     2161 ataccctggc gggtgtgagt cacttccggg tggaactgta cgccatagaa gcgtttttct
     2221 tcgttagcca taatggcaaa cgggcagctt tcggtgctgg ctacggtgac gaagtcggac
     2281 gggatagcgg taactttatc gccgtggctc atccagacat cgagcagcgg tttaccgtct
     2341 gcggtcagcg catcttcgat accgcgaacc agtgcgctgt cgtttacgac ttcaacctgc
     2401 gcgtagccaa attcacgttc gttagaggct tcaacgtgac cgcccaactg cattgccatg
     2461 gtctgcatgc catagcaaac gccgaatacc ggtacgcctg cttcaaagac atactgcggc
     2521 gcacgcgggc tgttttcttc ggtggtgctt tccgggccgc cggaaagaat aatgccgctt
     2581 ggattgaagt cacgaatttg tgcttctgtc acatcccacg cccacagttc gcagtaaaca
     2641 cccagctcac gcacgcggcg cgcaaccagt tgagtgtact gagaaccgaa gtccagaatg
     2701 aggatgcgat gcttatgaat gttttccgtc attgacgctt attccgaggc aagtgaaaca
     2761 gataatataa atcgcccgac atgaagtcgg gcgaagagaa tcaggagccc agacggtagt
     2821 tcggggactc tttagtaatg gtcacgtcgt gaacgtggct ttcctgaatg cccgcaccgc
     2881 tgatacgtac aaactccgct ttagtacgca gttcgtcgat agtaccacag ccggtcagac
     2941 ccatacagga gcgcaggccg cccatctgct ggtgaatgat ctctttcagg cgacctttat
     3001 aagctacgcg accttcgata ccttccggca ccagtttgtc ggcagcgtta tcgctctgga
     3061 aataacggtc agaggaacct ttggacatcg cgcccaggga acccatacca cggtaagatt
     3121 tgtaagaacg gccctggtag agttcgattt cacccggaga ttcttcagta cccgccagca
     3181 tggaacctac catcaccgcg cttgcgccag cggcgatagc tttggcgatg tcgccggaga
     3241 agcgaatacc accatctgcg ataaccggaa taccggtgcc ttccagggct tcaactgcgt
     3301 cagcaacggc agtgatctgc ggaacaccta cgccagtaac gatacgagtc gtacagatag
     3361 aaccagggcc gataccgact ttaaccgcgc tgcaaccagc ttctgccaaa gcgcgtgcac
     3421 ctgcagctgt tgccacgttg ccgccgatga tttgcagatc cggatattta gcacgggttt
     3481 cacggatacg ctgcaaaacg ccttctgagt gaccgtggga ggagtcgatc agcagaacgt
     3541 caacgccagc ggcaaccagc gcgtcaacac gctcttcgtt acccgcacct gcgccaactg
     3601 cagcaccaac acgcagacgg ccttgctcgt ctttacaggc gttcggttta cgttccgctt
     3661 tctggaagtc tttcacggtg atcatgccga tcaggtggaa ttcgtcatca accaccagcg
     3721 ctttttcaac gcgtttttcg tgcatttttg ccagcaccac ttcacgggct tcaccttcac
     3781 gcacggtgac cagacgctct ttcggcgtca tgtaaacgct aaccggctgg ttcaggtcgg
     3841 taacaaaacg cacgtcacga ccggtgataa tacccaccag ttcgttttct tcggtaacga
     3901 ccggatagcc cgcaaaaccg ttacgctcag tcagttcttt cacttcgcgc agcgtcgtgg
     3961 ttggcagaac agtctgcgga tcagtcacca caccagattc gtgttttttc acacggcgaa
     4021 cttcttctgc ctggcgttca atggacatgt ttttgtggat aaagccgata ccgccttcct
     4081 gagccagagc aatagccagg cgcgcttccg ttacggtatc cattgctgcg gaaagcatag
     4141 ggatattcag acgaatagtt ttcgtcagct gggtgctgag gtcagcagta ttcggcagaa
     4201 cggtagaatg agcaggaacg aggagaacgt cgtcaaacgt cagagcttct ttagcgatac
     4261 gtagcatggg caatatctcg accagagtgg ttaataaata ttgccgcggc attatacaga
     4321 gcgtaaccga ttgcatctac ccctttttgc aaaaaatgct tgctatcccc gaagggcggg
     4381 ttactatcga ctgaataacc tgctgattta gaatttgatc tcgctcacat gttaccttct
     4441 caatcccctg caatttttac cgttagtcgc ctgaatcaaa cggttcgtct gctgcttgag
     4501 catgagatgg gacaggtttg gatcagcggc gaaatctcta atttcacaca accggcttcc
     4561 ggtcactggt actttacact caaagacgac accgcccagg tacgctgcgc gatgttccgc
     4621 aacagcaacc gccgggtgac cttccgtcca cagcatggac aacaagtttt agttcgcgcc
     4681 aatattacgc tctacgagcc gcgcggtgac taccagataa tcgttgagag tatgcagccg
     4741 gccggtgaag ggctgctgca acagaagtac gaacagctca aagcgaagtt gcaggctgaa
     4801 ggtttgttcg atcagcaata caaaaaacca cttccctccc ctgcgcattg cgttggtgtg
     4861 atcacctcaa aaaccggtgc tgcgctacat gatattttgc atgtgttaaa acgtcgcgat
     4921 ccttctctac cggtgatcat ctaccccacc gccgttcagg gcgatgacgc accggggcaa
     4981 attgttcgcg ccattgaact ggcgaatcag cgcaatgagt gcgacgtgtt gatcgttggg
     5041 cgcggcggcg gttcgctgga agatttatgg agttttaacg acgaacgcgt agcgcgggcg
     5101 atttttgcca gccgcattcc ggtcgtcagc gccgtcgggc atgagacgga tgtgaccatt
     5161 gccgattttg ttgccgatct gcgtgcgcca acgccgtctg ccgccgctga agtagtgagc
     5221 cgtaatcagc aagagttact gcgccaggtg caatcgaccc gtcaacggct ggagatggcg
     5281 atggattatt atctcgccaa ccgcacgcgt cgctttacgc agatccatca ccgattacag
     5341 caacagcatc cgcagctccg gctggcacgc cagcaaacca tgcttgagcg cctgcaaaag
     5401 cggatgagct ttgcgctgga aaatcaactt aagcgtaccg ggcaacagca gcagcggtta
     5461 acacagcggc tgaatcagca aaatccacag ccgaagattc atcgcgcgca aacgcgcatt
     5521 cagcaactgg aatatcgttt agcagaaacc ctgcgcgtac agcttagcgc cacgcgtgaa
     5581 cgtttcggta atgcagtaac gcacctcgaa gccgtaagcc cactgtcaac gctggcgcgt
     5641 ggatacagcg ttactactgc tactgacggc aatgtactga aaaaagtgaa gcaagttaaa
     5701 gcgggtgaaa tgctaaccac acgtctggaa gacggctgga tagaaagtga agtaaaaaac
     5761 atccagccgg taaaaaaatc gcgtaaaaag gtgcattaag ccagcacgaa ttcaacgcgt
     5821 tttttcgata tcaagccatg cccgtgctga cagaagtaat ctactgcacc gcaggctttt
     5881 aacacctgca acggttgatg gcagtctgga caaagagctt tcatttcaat aatttccccg
     5941 caggatgggc aacgcgcatg gccgttatcc tgatcaagca catgctgaca ttgtggacaa
     6001 tgcagttcca tatgaattcc tccggtagcc atccctctac attcatagag ggaatggcag
     6061 ataaaatact tacggataat tatttatttt tcttgatgtg cttcatcaga cgcttacgtt
     6121 tacgcatctg ggttggcgtc agggtgttac gcttgttcgc atacgggttt tccccttctt
     6181 tgaactgaat acgaatcggc gatcccatta cgtccagcga tttgcggaag tagttcatca
     6241 agtagcgctt gtaggaatca ggcaggtctt tcacctgatt accgtgaatc accacaatcg
     6301 gcgggttata accaccagcg tgggcatatt tcagcttcac acgacgaccg cgtaccagcg
     6361 gcggttggtg atcttcaaca gccatcgtca tgatgcgcgt cagcatagag gtccccacac
     6421 gacgggtgga gctgtcatac gcttcacgta ctgattcaaa caagttacca acaccactgc
     6481 cgtgcaaggc agagataaag tgcacacgag caaaatcgat aaagcccaga cggaagtcca
     6541 gcgtttcttt cacctgctct ttcacttcct ggctcaggcc atcccactta ttcaccacaa
     6601 tgacaagtga acgcccacta ttgagaataa agcccagcag cgagagatcc tgatcggaaa
     6661 taccttcgcg cgcatcaatc actaacatca ccacgttggc gtcttcaatg gcctgcaacg
     6721 ttttgattac ggaaaatttc tctacagcat cggtgatttt gccgcgttta cgtacgccag
     6781 cggtgtcaat gagcacatac tcacgtccat cgcgttccat tgggatgtag atgctgtcac
     6841 gcgtcgtgcc aggcatgtca taaacaacaa cgcgctcttc accaagaata cggttagtga
     6901 gtgtagactt acctacgttc ggacgaccca caatcgccag tttgatcggc agactttgcg
     6961 ggtcgaagtc gtcttcctct tcttcttcgc cgttctcttc cgcttcaaat tgcgcccagt
     7021 attcagcgtc ttcgtcgact tcctcttgcg gtgcgagatc ttccatccac ggcagcagca
     7081 catgctccag cagacttaat acgccacgac cgtgagacgc ggcgatcggg tagatttcac
     7141 ctaaaccaag cgagtagaaa tcaaccactg cctgatcggg atccagaccg tcagttttgt
     7201 ttgccaccag gaaggtcggt ttttcacggg agcgcagatg tttggcaatc gcttcatctg
     7261 ccggcatcag gcccgcgcac gcatccacca taaacagtac gacgtccgct tcttcaatcg
     7321 ccagcagcga ctgttccgcc atgcgggttt ctacaccgtc ttctgtgcca tcaatcccgc
     7381 cggtatcaat acagataaac tcacggcctt caatttccgc acgaccgtac ttacggtcac
     7441 gagtcagacc cgggaaatcc gcaaccagcg catctcgggt gcgagttaga cggttaaata
     7501 acgtggattt tcctacgtta gggcgcccga caagcgcgac cacaggtacc atgtttaaag
     7561 cctcattttt ataaatcatc agacaacgca cgctatattc gcgtcgttgt taaaaacagg
     7621 aaaacggccc ctgtccagga gccgttttca aagtgaacga cagagacgat taacgtgtaa
     7681 tagagtacac ggttccgtct tttgcctgga tcagcagttt gccgtcagcg gcaaccggtt
     7741 cagtctggaa accggaacta tcaacttttt gctgggcaac gaaacgacca tcttcgacgt
     7801 taatccagtg cagataacct tcactgtcac cgaccaccag gttgccatta tacagcaccg
     7861 gagaagtcag caggcgatgc agcagatcgc tttgtgccca cagcgtaacg ccgccatcaa
     7921 tggtcaacgc catcacccgg tcattttgat cgaccagata gatgcgattg ccgtcgacga
     7981 tgaaatcatt caccgaaccc agttcgcgtt tccacataat ctgaccactg cgcagatcaa
     8041 gcgccgtcag gttaccatta taggccagcg cgaaaacaac gccgttaaca acgacgggag
     8101 tcgtgtcaac atcgctcaga cggtcaattt cggtagaacc ggtcgcctgg gaaatacgct
     8161 gctgccaaat catctggccc tgttccatca gcactgcgct gacgcgacca ttatcgcccc
     8221 ccacgacggc cgcaccaaaa gccgttgccg gcgcagactc gccacgcaaa gagagcgaag
     8281 gcatatcgag gttaactgtc catttgacag cgccgtcagc ttcgttcagc gcttgtaact
     8341 gaccgttact ggtgtggatt aacaccagac cgtcgctgac caccgggcgc gaaagtgctt
     8401 cacccgcgac tttagtttgc catgccacag taccatcgct ggtattcagc gcgtaaacct
     8461 gcgccttttc gctgccaatg tagacatgcc caccagacac ggtcacaccg ccagaaagta
     8521 atgcaggctc tttagagaac cagccatctt tctcggccag gctgacagac cagatttctt
     8581 tgccatcatc cgcattcagc gcttttacca aaccagcgcg gtccgctgca tagacaacgt
     8641 tgtccgccag tgccggatga agattggaat agaagttgcc aatgccgcta ccaacggaag
     8701 tactccacgc cgtggtcggc gtaaactggt tttcaacggt tggcaatggg gacatcttta
     8761 ccacatcttc ttcgctgtta aacagcgaac agccgcttaa aagggtaacg gaaagcagtc
     8821 ctggcagcag taatttacgc aattgcatcg ggtccctctc agatggacaa attattaatt
     8881 ttcatctgca tcatttcgct cagtgccgga gtaacatcgc ttttcacgcc tgcttcccat
     8941 gcactacgcg caccttgctt atcacctttg ctcagcaatg cttcaccacg caggtcggca
     9001 acaatggcag cccacccttc acctttgatg gtatcaaggg ttttcagcgc ggcatcagcc
     9061 tgcttgagct gtacctgaac gcgagcaaga cgcagattta tcacggcttt gagattttca
     9121 tcgctcgtgt ctgccagccc ctgttgtaac tgggcggcag ctttctctag ttcatttttg
     9181 tcaacaaatt gctgcgcaag ttccaaagaa gccagcgcac cataagtatt tttattttca
     9241 gcagcaaatt tttccgcagc cgggatgcta tccggtttgc cttcgctcac tgcggtaacc
     9301 gcattttgat aggcaagaga agcggagcgt gcagaatcaa cctgatggct gttccagtag
     9361 cgccagccaa tcagtgcgcc aacgcccaaa atcaccccaa cagccagtgc tttgccattt
     9421 tcagcaaaaa agcgtttaac cgcttctacc tggtcgtttt cgttctcgta aatttccacg
     9481 ctgtccttct ccttccttaa cccagtaacg tgcgcaaatg cgcggctacg ctatcctgcg
     9541 caactgccgt ttgctcacca gagcgcaaat ccttcactac tgctgtgccg ttagccactt
     9601 cagactcacc cagcaccaca gcaacgcggg caccccattt atcagcacgg gcaaactgtt
     9661 tcttaaagtt gccgccgccg tggttggtca tcaatttcac gcccggtaat tcatcacgca
     9721 gacgctcagc taatgccata gccgcagatt gtgtatcagc acctgaagcc accaggtata
     9781 tatcgacaac aggatcggct ttaaattccg gattaacggc ctgtactaac aatacaagac
     9841 gttcgaggcc catcgcaaaa ccgacagccg gtgttgcacg accgcccagt tgttccacaa
     9901 gaccgtcata acgaccgcct gcacacacgg tgccctggga gccgagactg ttagtcaccc
     9961 actcgaaaac ggtacggtta tagtaatcca gaccacgcac cagacgctgg tttacggtgt
    10021 aagcgatccc cgcgctctca agcagtttgc acagaccggc aaaatgctca cgagattcct
    10081 cgtccagata atcacctaat gccggagcgt cgttgagaag cgcctgcact tccggatttt
    10141 ttgaatccag cacgcgcagc gggttagtgt acatgcggcg tttgcagtct tcgtccagct
    10201 tttctttatg ctgctcaagg aatgccacca gcgcatcgcg gtaattggcg cgtgcttcca
    10261 gcgaaccgat agaattcagc tcaagagtta cgtgttcgga aatacccagc gcgcgccacc
    10321 agcgggcggt gagcataatc agttcagcgt cgatatccgg accttgcaga ccgaaaactt
    10381 cgcagcccaa ctgatggaac tgacgataac gccctttctg cggacgctcg tgacggaaca
    10441 tcggcccgat ataccacaga cgctgttcct gattgtacag aagaccatgc tcgatgccgg
    10501 cgcgtacaca gcccgccgtc ccttcagggc gcagagtcag gctgtcgcca ttgcgatcct
    10561 caaaggtgta catctctttt tcaaccacgt cggtgacttc accaatcgca cgtttgaata
    10621 gcggggtctg ctctacaatc ggcaagcgga tttcactgta accgtagctg ccgagcacgt
    10681 ttttcagtgt gccttcaatg cgctgccaga tggccgtttc gccaggcagg taatcgttca
    10741 tgccgcgaat ggcttgaatg ttttttgcca cgtttattct ctttctgaat ataaaaatga
    10801 accctcaacg cttccctcaa tgtttcggga gctatgcggg ttcaatcata cacgggaagc
    10861 gaggcgcttc ccatcacgtt attatttttc aacctgctga acgtcaattc gacgcgcttc
    10921 gtccagctga ctggctttcg cacgaatgcg tgcttccagc tggtcgatca tatcgttgtt
    10981 gtccagacgg tctttgcgca cgccatcttc atagaggccg cttttcttgt tgccgccggt
    11041 gacgccgagt gtagaaacca gcgcctcacc tgggccattc accacgcagc cgataatcga
    11101 aacgtccatc ggagtgatga tatcttccag gcgttgctcc agcgcgttaa ccgtaccgat
    11161 aacatcaaat tcctgacgcg aacaggtcgg gcaggcgatg aagttgatac ctcgcgaacg
    11221 gatacgcagc gatttcaaaa tatcgaaacc gactttgatc tcttcgaccg gatcggccgc
    11281 cagtgatacg cgcagcgtgt cgccgatgcc ttcagacagc agcagaccta aaccaatggc
    11341 ggattttact gccccgctgc gtgcaccacc ggcttcggtg atccccagat gcagcggctg
    11401 atcgatctgt tttgccagca aacgataaga ctcaacagcg aggaagacgt cagacgcttt
    11461 cacgctgact ttgaactgat cgaagttcag gcgatcgaga tgatcaacat ggcgcatggc
    11521 ggattccagc aacgcctgcg gcgtcggttc gccatacttt tcttgcagat ctttttccag
    11581 cgatccggcg ttaacgccaa tacggatcgg aatgttttta tcgcgcgcac agtcaaccac
    11641 catgcgaata cgctcttcat taccgatatt gccagggtta atacgcagac aatcgacgcc
    11701 gtattccgct actttcagtg caatgcgata gtcgaagtgg atgtcagcca ccagcggcac
    11761 gttaacctgc tgtttgatga gtttgaacgc ttctgccgcg tccatcgtcg gtacggagac
    11821 acggacgata tcagcgccaa cgcgttccag cgccttgatt tgattgaccg ttgcttcgac
    11881 gtctgtcgta cgcgtattgg tcatggactg tacggcgatg ggagcaccat cgccaatcgg
    11941 cacattccca acgtaaatac gtgttgattt tctacgttga attggagcct ggttatgcat
    12001 gaaaaatctc ccgcgttacc cgtctgttac tgcgccggtg attgttcggc attgagggtc
    12061 agacgcgcaa cctggttagt tctgataaaa cgactcagat cgacaggttt cccttgatac
    12121 tggatctgta ctgcggctgg cgcaccaatt ttcagtttgt atggtgcctg gccggttaag
    12181 ttcaaattac cgtctttacg ctgcataccg ctaaacaatt ttttaccggt agcatcagta
    12241 acctccagcc agcaatcggc agtaaagttc atcaccagcg cattcggatc agccgccggc
    12301 gtagtcacgc ctgcctgatc ggttggcaac ggcgcagcgc catctggcgt tgttgttgcc
    12361 gttggtgccg gggtcgcggc ggtatcaaca tttgcttgcg aaggcgaaac aaccgcattc
    12421 tgttgcggat caacagctgg tgctggcgca gttacggcag gtgtttgcgt gttggttgcg
    12481 gtagtatcca cagacgctgg cggcgtggac gttgtagccg ggtctgtagt tgtcgacgta
    12541 tttaacggaa cactctgccc ctgctcgcta ttactgctca gttccgccga agattgatcg
    12601 gccatagtgg tgatctcttc ctgctgagct ttgtggtctt gccaccacca ggcaccgctc
    12661 aggccgataa ccacaaacaa caccagccag gtgaaggtca tcagccagcc gtcacgtttt
    12721 ttgcggcgtt taccgaggga aaaactctgc ataggcgcaa cttttgcagc ccgtagtgga
    12781 gcctgctttt ccagccctgg cagcagttct tcttctggaa tatgtaccag acgcgcataa
    12841 gagcggatat atccgcgcag gaatgttgaa gcaagatcgg cgggtgcctt atcttcttca
    12901 atgtcgcgta ccgtggaaac cttcaggcaa agtcgctcgg caacggcctg ctgactaagt
    12961 cctagttgtt cgcgagcatt acgcaggcga gcgccggtag taagtgcttc attttggtcg
    13021 tgcgtggctt cagtattcat tcgctgcagg tacgtttaaa tgagaattga gatgccggtg
    13081 aaccatcatg ctcacccaca ccgcgaaaca tccgttaagt taaccgttat catacagtat
    13141 aagaccgtct gtgtgctcgt gacaaagccc acattcacta aggctaactt actgttgcat
    13201 cgttacatac tgccttaaag tcagcaaaaa cgcaccgtta atattgacca gacaattgca
    13261 acttaatact tcattcataa aagattacgc cacggtacat aaagtaaccg tggcgtaatg
    13321 gctatcagac cgctttaatg tcgatggctt caccctgcat ccgtttacgc agggtacgtt
    13381 tcgtacggtc gataacatcg cccgccaact gaccacaggc agcatcgata tcatcaccac
    13441 gggttttacg cacaatagtg gtgaaaccgt agctcatcag cacttttgag aaacggtcga
    13501 tacggctgtt cgagctgcgt ccatacggcg cgcccgggaa ggggttccac gggatcaggt
    13561 tgatcttaca cggcgtatct ttcagcagtt ccgccagttg gtgcgcgtgt tcagtgccgt
    13621 cgttaacgtg gtcaagcatc acatactcaa tagtaacacg gccctgattg gcgttggatt
    13681 tctccagata acggcgcacc gcagcaagga acgtttcgat attgtacttt ttgttgatcg
    13741 gcacaatttc gtcacgaatt tcgtcgttcg gcgcgtgcag ggaaattgcc agtgcaacgt
    13801 cgatcatatc gcccagttta tccagcgccg gaactacacc ggaagtagaa agcgtgacgc
    13861 gacgtttaga caggccaaaa ccgaaatcat caagcatgat ttccatcgcc ggaacgacgt
    13921 tgttcaggtt gagcagcggc tcgcccatgc ccatcatcac tacgttagtg attgggcgct
    13981 gaccggtgac ttttgctgcg ccgacgattt tcgccgcacg ccacacctgg ccaataattt
    14041 ccgatacccg caggttgcgg ttaaagccct gctgggcggt ggaacagaat ttacactcca
    14101 gcgcacaccc cacctgcgaa gagacgcaga gcgtggcacg gtcgtcttcc gggatataca
    14161 ccgtttcgac gcgctgatcg ccaacggcga tcgcccattt aatggtgccg tcggatgaac
    14221 gctgttcttc aaccacttcc ggtgcgcgga tctccgccac ctctttcagt ttgccgcgca
    14281 acactttgtt gatgtcggtc atctcatcaa agttgtcgca gcaatagtga tacatccact
    14341 tcatcacctg atcggcgcgg aagggttttt cacctaaatc tttaaaaaac tcccgcatct
    14401 gctgacggtt gagatccagc aggttgattt ttccatcttt cgtggtgacg ttttcaggtg
    14461 tgactaattg ttcagacata tgctattccg gcctcgttat tacacgttat ggcccctgga
    14521 gggttgaaaa aagaaacgcc ccggtgagcg gctgctcgtc cgggggcgct gcattgtaca
    14581 aattctggcg cacggatgcc acgtttgcac gcggcattta cgaaattatt aacgggtgcg
    14641 cgggcacact tcgccttcgc caaagaaata agcgatttcg cgagcggcag attcgacgga
    14701 atcagaaccg tgggtaccgt tttcggtcag gctgtcagcg taatcagcgc gcagagtacc
    14761 agccagtgcg tttgccggat tggtcgcgcc cagcagatcg cggtgacgct gaacggcgtt
    14821 ttcaccttcc agcacggaaa ccacgatcgg gccagaggtc atgaattcaa ccagaccatc
    14881 aaagaacggt tttccatcgt gttcagcata aaagccacgt gcctgttcaa cggtcaggtg
    14941 cagcattttg gtgccgacaa ttttgaaccc tgcagcttca aagcgcgcaa agatattacc
    15001 aatgacgttt tttgctaccg cgttcggttt gatgatggaa aaagtacgtt caatagccat
    15061 ttttacctct gtaaattgtt ctgttgttgt ctgtaccagc gtacgagatg gcgcggatta
    15121 taatgagcaa cagggccgtt gactattgat gaaggtaaca tttttttaaa ataaaatgag
    15181 ttttagcaac aatcaggggc aaaacagact attgcatgac aaatttcact gtcgcgattt
    15241 gccccacatc atccatcacc agcaattgat aatcaccttt atccgtcaaa tgcagggtca
    15301 cgttgcgccc gcgttcagtt aacggttcgc cattcagaaa ccaccagcgt tcacctgccc
    15361 ctccacttga ttgcaacggc aaagtcgctt ccgctgcgcc cggtaaacgt ttaataatcg
    15421 cgccatcgcg aacgcctgtc agctgcagag ggagcttggc atcgtgaccg tatggcgggc
    15481 aaattgtcga ggccggtggt aagcgcacag cgcgacgttc tgatgcgggc agccagggtt
    15541 ccagcggtag cggccagaca tttatcattt cttgtcgcgc ttgcgggcaa tcagcggcaa
    15601 cacgtttgcc attttcatcc agccagatgg ggaaacgaat gccattaatg ccttcctgct
    15661 ccggcagtaa tagagttggc ggctgactcc cgtccagcag ccaggttgcc aggcggcggc
    15721 gacagttacc gtcaccttcc ggcaaagact gtccgcccgg ccagcagata acgccacgag
    15781 tgactgagtt cgggcgcggg tcttccggca gattcgcact gcgcgacagt aagatattat
    15841 tgacctgatt caacaatggt acggcactgg caaagccaaa ctgaccaaca acgggcgtgc
    15901 cgtccggtct gccagtccag atcccaatga catagcgagc gttaacccca atcgcccagg
    15961 cgtcacgata gccatagctg gtgcccgttt tccatgccag tggggcgacg cgcggcaagg
    16021 cactatccgg caagggtttc gcttcatcag ccataatccg gcgaatgatc cacgccgccc
    16081 ccgacgacat taaaggccgt tcaagcagcg gatcgtcagg ctgtaagcgc aatttgcctg
    16141 ccttgccgtg gcgagcaaac gcggtatacg ctgccgccat atcttccagt tttgcaccag
    16201 cgccgccgag aatgagtgaa agattcggcg cagcaccgtt gggcaaatat aacggcaatc
    16261 caacattgcg taactttgcc gcaaaccgtt tcggtccata ggcttccagc acctgcacag
    16321 caggtaagtt cagcgagcgc accagcgcct cgctcatgct gatcgggcca tgaaaaccgc
    16381 tatcaaagtt acctggtcga taatcaccgg tgcgccgggg gacgtcttgc agcagtgatg
    16441 ccgggtggat caagccttca tccagcgcca gaccataaac aaacggtttg agcaccgatc
    16501 ctggcgatcg gatcgcattg accatatcaa catgaccaaa gcgtgaatca tcgttgagat
    16561 caaccgatcc cacccagccg cgaacacgca tatcggtatg atcaaccacg atcatcgcca
    16621 gtgagctgcg cggtggcaac cgccctttcc agttttgcgc cagttcttcc agacgtcgtt
    16681 gaagaccggc atccagcgta gtaacgattt tgtcgctttt gcttttaccg agcatcatgc
    16741 gcgaaaacag cggtgccagt tgcggcattt gtcggggtgc cagccagatg ggttcttccc
    16801 ttgactcttt tacctgctca cgggaccaca caccttgcac ggccatccgt tcgagcactt
    16861 tattacgcgc ggcttcggca cgctccggcc aacgatccgg gcgaagacgg ctgggcgctt
    16921 gcggcaaaac cgccagcatt gccgcctcgg aatagcttaa attcgcaggc gactttccga
    16981 gataagccca acttgccgca ccgatcccct gcaacgtacc gccaaacgga gcgcggttaa
    17041 gatacaaggt cagaatttca cgcttagaca gatgccattc cagttgcaac gcgcgccaga
    17101 gctggcgaat tttgccgcca aatgttttgg ggtgaggatc aagcagacga gcaacctgca
    17161 tagtgagcgt gctgccaccg gaaataaccc gtcccgaagt gagatcttgc catgctgcgc
    17221 gcgccaccga gaatggattc acccccggat gcttccagaa ccagtgatct tcataattga
    17281 tcagcgcttc aaggtaacgt ggagaaacat cttcgattgt taccggataa cgccagatgc
    17341 cgtcagcatc ggcgaagcgc catagcggcg taccatcctg cgccacgacc actcgtgcgg
    17401 gattgacttc atgcagcggt agaggccaga gtttatctgc tccccacgct gcaagaaaaa
    17461 ggagaaatgg cgcagctgcc agcgttatcc agcagccgcg tttggttaac aagcgaggca
    17521 tttacggtcg gacaatcagc agatcttcag ccgcgccggt cgcccgccat tggggaacat
    17581 acattgattc caccatcggt tgcggtacct gatacgttcc cggcgtcacc gcccgcgcca
    17641 gatacaccag cgttaccggt tggtattcat caacggcaac cgccgccaca aagcgatcgt
    17701 cacggaactc aatgtgctta atactcgcct gctgcatctg gttcagtaag ttctgcactt
    17761 cgccaccact ttgctccagg ctggcgctac cgttcgccag attctgattt tccagttcca
    17821 gacccgcagg cagcagatcc acgactaacg catccggcac gctgttactg gcttttacct
    17881 gcaaccagac cagcaccaga tcgccgctac gtaacgagtc cagcgatttg ctcttaccat
    17941 cagtaccaag aatatgacgc tcgatttgca gcacattgtt cgccggtaaa ggtgcggatt
    18001 gcggataacc gctggcatcc atacgcaacc ataacggctg atcgccactg tttctcacct
    18061 gcaaggtggc aagttgatcg ctattcagat tgctgtttag cgttttctcg cctgtcagcg
    18121 gctcagctga gaaagaggtt tgcgcctgcc atttacccgg taaatcctga atcgtacggg
    18181 cagctaggaa caacgcgtta ctttcctgcg tcgatagcca gcgttcacca aacgcctgct
    18241 gcgaaagtgt gttcagcaaa gtgtactgct catcgggtag cagtttattt tcttccagca
    18301 aggagagcat taacgcgttg tcgcgcagtg gactaccgta atcacccagc catatccgct
    18361 catcactatt tctcggcgtt ttcagcgcca gcacaatcgc ttcttcacca cgcgttgcat
    18421 cacccatggt tttcagcgca acgccaagtt gcagcagcgg taaaccagaa gcggcatctg
    18481 cacgatgctc ccagatttca cgcagcgcac ccagcggagc cttttgttga cgggccaaca
    18541 ccagcgcagc gtaagactgt acggcgaatt tactggcttt gagattatcc gcgtacggga
    18601 tcgacatcat gcccggatct tgtaaatagc gcagcagacg ctcattaccc cggttaatgg
    18661 cgtctgtcgg cacgctgtaa ccctgttcgc ctgcgcggac caggaaatcc atcacgtaag
    18721 ccgtcagcca gtactcttcg tcaccgtttt tatcccacag cgcaaagccg ccgttatcac
    18781 gttgcatttg cagcaaacgg gaaatgccga tatcgaccga tgcacggcgt ttctcatcac
    18841 tgtcgccttt gatgcccaac gcctgcagtt gggctgcgtt ggtataaagt gacgggaaca
    18901 ggccgctggc ggtttgctca agacagccgt acggatacgc ttttaactct ttgatataac
    18961 gtgcaatgtt cagcggtggt ttgccgctca acaacaattg cccttccagc gtaacaggcg
    19021 agaagttttg caatccatcc gccggaatcg cccatgtctc accaggctgt aacgccgtac
    19081 cgtaattaac cgtttgtgcc gggaacgccg gacggacgcc gattttccac tgcttctgct
    19141 gatcggcaac ggtttcatcc ggtaacgcta acccgctaat ggtggcctga atttctccat
    19201 cgccataacc tggcaatgct cgcaccggga taaacagcgt agtacgcaca cctggcgcta
    19261 attcaacggc tgcgggtgaa tcgctgacca gttcaagcaa accactggcg gtcagggcaa
    19321 cgttcagttt ttgcggttta tcggtaagat tagtgatatc cagcgtcaga cgcgaggtat
    19381 cgccactcgc cataaagcgc ggcatgttca gttcagcaat caccggtgcg gcaactatca
    19441 ctttgctttc gttgctaccg aagtcatctg ccgtccaggc ttgcgccatg acgcgtaatt
    19501 caccgttaaa atcgccaatc ggcagtgtaa ccgagccttc gccctgttcg ttgagcgtta
    19561 ccggcagcgc ctgctgcgcg acaatattga cgtgattgac cggcggttta ccaccacgtt
    19621 tcagctcatc accatcgcca ccgaaacgca gagctgccag acgcccctga ccttcaataa
    19681 cctgaccgta aatatcgtaa atgtctgcgc catagcgttt ctgaccaaag aacgcctgcc
    19741 acggatctgg cgtgacgtag tcagtaatat tcagcacacc actatcaacg gcagacacca
    19801 gcacattcac ctgtttaggc ttctcgccat ttttagtgct ggctttaatt ttcacggtta
    19861 atggttgatt cggccgcatt tttgctggtg tttccagcgc cagatcgagg cgacggtttt
    19921 catcgccaag cggcagatgc aacaccccaa ctgcgcgttt tggcgtcgcg gagcgagatt
    19981 tatcgccagg acgtactacc agcgtactta aatacagatc atgacgattc caggttttat
    20041 cgaccggaat cgtcagatcc agcccttgag ccggaacatc aatctcttgc caccacaacg
    20101 gcccttcact ggactcgacc atcgcataac ctttacccgc cgttggcgcg gcaatatgca
    20161 acttaatggt atcgccaggg cgataactgg ctttatccag tttcagcgtg acacggtcgg
    20221 gtcgcacggc accgctcccg tcgctgttgt cctgccagct atagccagcc cagaaacgca
    20281 cgctactgac cgcttcattc ggcgctttga cttccagacg ataagcgccc cactctaccg
    20341 ggaaactgac cttaccggtt tcatccgcct gaagatccag agtttgttca ttttcgatca
    20401 gatctttttg atcaaactgt gactgccagc cttcatcttc tgaccagttc cagtagtaat
    20461 cgcggcgttc gcgaatcagg cgcacctgca agcccgacac ggcttttttc acgccttgcg
    20521 catcgctata aacgatgtca aaaccggcgt tactgccttc atcaacaatg ggctgtttca
    20581 ccgtgctgtc agtgcgataa tcgtaaaccg atttcgaggc gaactgcgga cggatccccg
    20641 gcaatgcatc ggcaggccag atagcctgct cagcgcggcg cgtcaccggg cgaccgcccg
    20701 attccagcaa actaccctgg aaaataacct gtaatgggga atgcgtttcc ttccactggc
    20761 tttctgtaga aacttcgccg cgccctttat catccagcgt caactgaact tcatccagcg
    20821 tgcgggaaag attttcggca gctatatcgc cgaattcaaa accaggtaag gctgacacag
    20881 cttcacgcag tgggcgcagg aaaagttgcc cttgcaaagt attaccatta gcaggtgcac
    20941 catacaggta gtaccccacc acggagaatt tcacttcatc tttcggcgtt agcggggttt
    21001 tctcaccggt cagattcagc gccatgcgct ctggcataaa atcttcgacg tggaaatccc
    21061 acatccgata ctggttatcg cccgtgttag cgcgaatatg ccacataccg gttgccgcat
    21121 tgctatcgag tggccaggta aagtggtaga ggccattctc cggctgacta acgacgctcc
    21181 tgagtacctg cccatcgggt ttaatcacgt ctaacttgat gggttgattg ggcaacgctt
    21241 taccgtctgc atcacgcagc aaaccattga ggattaccgt ttcacctggg cgataaagat
    21301 cgcgtgggcc aaacatgaaa aactgtttgc tatagcctgg cgcgccagca atgttaaatt
    21361 ctgctaagtc cagcgccgga agttttaaat cgagtagcgt tgtctgaccg tctttacgcg
    21421 ccaacagtaa tgccgcgttt ttatcatttt ccagctgcac atgcccctga gcgtcactgg
    21481 ttgcctgagt cagagtcagc cctttctcat ttaataaaga gacttcaatt ccttgctggg
    21541 ccgcgccgtt ttccagactt tgggtaaaga tatccagacg attgtgataa cggtgagctg
    21601 aaacgccgat atcacttaac gtaaacagcg tcgcgggatt actgtaatcg taacgtccag
    21661 cctgattcat cacagccaga tacacgcccg cctgctgaag cggtttgata tcgcccagcg
    21721 gcagcaataa tttttcacgg gtgttacgcg caggattgag atcaaaccgt ccggtgtaga
    21781 ccagatccgc catctgcagc agtttgtctg actgccagtt cgccagcgaa ttgcggtatt
    21841 cccattggct aatgaatgct ggcagagatt ctggcttaac gcggaagaag ttaacatcga
    21901 cattattaac gttgagcgcc attaccggca gcccttcaac gactttgcca ggcagcagcg
    21961 aaccacggct ggcaaaaccg acgctgggtt ggatgtcgcg ggtagttata gttttttcgt
    22021 aatctttact gaaggttgcg ttgttgagtg ctttgacctc cttgccaata gtaacgatca
    22081 aatcacgttt cggttcgagg tggcgtaaac gtaactcttt aagattatct gacagctccc
    22141 aggcaccatc cactttgccg ctttttttat cgacgacatg aataacgcgt gagaaatcct
    22201 gatccgggtc gagagggatg gagaacgtca gcaccagcgt ggctgcacca tcaagttgga
    22261 cttcagagag atccagcaat gtcagcacct taccggcact ctgttgggcc agtttttgtc
    22321 tctccggcac ggagagtttt gctgaactcg cgttttctga agaggccgct ttagtaactt
    22381 cagaaggcgc atcttttttc accgctgttg gcgcgttatc gttgttgtcg caccctgcca
    22441 gcgccagcat tagcatgcag gcggctacgc gtaacttttt catttttcat ccctgccaca
    22501 atggcccgtt agcaacgtcg aataattatt atgcgtgaga atttacgtta tgtaattcag
    22561 tatcacgccc atacaatccg aattttagtg agaattgtgg ctcagacagc caaatcagcc
    22621 tcttttgctt gtcgcttttc actaaacatc cgacaattta gcctaacccc ggcaaaaatg
    22681 gagatgccta tgtccacgac atggtttgta ggagccgact ggctcgccga acatattgat
    22741 gacccggaaa ttcagattat cgatgcctgc atggcgtcgc ctggacagga ggatcgtaac
    22801 gttgctcagg agtatctgaa tggacatatt cccggcgcag tgttttttga tatcgaagcg
    22861 ctttctgatc acacttcccc gcttccgcac atgctgccgc gcccggaaac gttcgccgtg
    22921 gcgatgcgtg aattaggcgt taaccaggat aagcacctga ttgtctatga cgaaggtaat
    22981 cttttctcag ccccacgagc atggtggatg ctgcgcacct ttggtgtaga gaaagtgtcg
    23041 attctggggg gtggacttgc aggctggcag cgtgatgatc tgctgttaga agaaggtgca
    23101 gtagagctgc cggaaggaga gtttaacgcc gcatttaatc ctgaagccgt ggtgaaagta
    23161 accgatgtat tattggcaag ccatgaaaat acggcgcaag ttattgatgc ccgcccggct
    23221 gcacgtttta acgcagaagt tgatgaacct cgcccaggtt tacgtcgcgg acatattccc
    23281 ggtgcattga acgttccgtg gacggaactg gtgcgcgaag gcgaactaaa aacgaccgat
    23341 gaactggatg cgatattttt tggtcgcggc gtcagctacg acaaaccaat tatcgtcagc
    23401 tgcggctctg gtgtaacggc agccgtggtt ttgttggcac tcgcgacgct ggatgtgcca
    23461 aacgtgaaac tgtacgacgg cgcatggagt gaatggggcg cacgggcaga tttaccggtt
    23521 gagccagtga aataagcttt atacaggcaa taaaaaaccg ccgaatttgg cggtttttta
    23581 ttgctggtct ggttcgcggc ctttccagca ggttgtatta ccgtagtaat gcaagcgcgt
    23641 ctcagcggag acaatactcg ccagtaactc tctttttgtc aagcaaaaga gagttattat
    23701 tgttctgtta gtgtattatc cactgcggcc ctttccgccg tctcgcaagc gggcgctggc
    23761 ttaaggaaag gatgttccgc gaccgttaga gcaagtgcgt ctcagcgctt gctatcgcgg
    23821 caatatcgcc agtggtgctg tcgtgatgcg gtcttcgcat ggaccgcaca atgaagatac
    23881 ggtgcttctg tatcgtactt attgtttctg gtgcgctgtt aaccgaggta aataataacc
    23941 ggaatctctc cggcgacaat ttactggtga ttaacaacct tcagagcagc aggtaagccc
    24001 gaatgccgcc ctttggggcg gcatatttta gattatccga ttctgtttaa agtcacgcaa
    24061 aaaaccaccc cagcgacgtt catagaatgg cgcaatatgt tcggtaataa agtggctaat
    24121 tcctttttcc ccttttttca cctgacaaat atcgatgggt tcatcgccag gtaatgtatc
    24181 ggtcgctaca cttcccgcca cctgaataat ttcttcgata tcaccatcgg cttcaatgcc
    24241 aataagtaaa ttaggctgtg cctcttcgtt ctctttaatt gaacaaataa aagcacgctt
    24301 cacaggctta atggttttaa ataaggtggt gagtgaatca atcatttgtg ctggcggctc
    24361 tgcgacttcc gataatatca gcgattcacc gccttccagg atttcctggc tgctcagcgg
    24421 atttccctct tcaccaatca acaaactgat ttcacgcggc ataaattctt taccggttgg
    24481 cagtttggca ttaaggaaga gcgtttcgcc aagtgtcatc tcaaacaatg tgcgaacggg
    24541 cattacgaca aatgcctgtt cgtcttcaac cgcctgttga agtgcttcta acgaggtgaa
    24601 aaaaggaatg acgctggtgc cgtcttcttt ttcccagtgc tgtaaatcaa gcgcgctatc
    24661 ttcaaccaca gcctcgccct gcgccgccgt accaggcacc cagacggtgg attccagtag
    24721 agtacggaaa aaggccgggc ggtgcgccgg ttcggttgct gctttttcca gcaggtcttc
    24781 aagttcgttt tttgtttcgg acataagaac cacaattcat tcaacgttcg gggcgcaaat
    24841 gcctgatgcg ctacgcttat caggcctaca agggattcgc aatttgttga atttgcagaa
    24901 tttgtaggcc ggataaggcg tttacgccgc atccggcata ttagtttacg ccgttaacag
    24961 attagctatc gtgcgcacac caagtcccgt agcacccgca gaccactgtt caaccggcgc
    25021 tttacggtaa gtcgccgagc agtcgatatg cagccagcct tgctgatagt tctcaacaaa
    25081 gtgcgacagg aagcccgccg ccgtgctcgc gcctgccgga tacgccgcgc ttccggtatt
    25141 gttcagttcg gcaaagttag acggcagctg gctgcggtgg aattccgcca gcggcagacg
    25201 ccagaatggt tcgttctctt gtgaggcact cgccagcaaa cgaccggcaa gcgcatcgtc
    25261 aaaactgaac agcgcgtgat aatcattacc cagcgcagtt ttcgccgccc cggtgagggt
    25321 cgccgcatca atgatcattt ccggtttctg cgcactggca tcaatcagac catcggcaag
    25381 caccagacgc ccttcggcat cagtgttcat cacttcaact tttttaccgt tgcgataggt
    25441 gatgatatcg cccagcttga acgcattgcc gctaatcagg ttatccgcac agcagaggaa
    25501 cagcttcacg cgcttgttca gtccgcgcgt aatggcaaat gccagcgccc cggtaaccgt
    25561 tgccgcgccg cccatgtccg acttcatcga gtccataaac gcagtctgtt tgatgctgta
    25621 gccgccggag tcaaaagtga tacctttacc taccaggcac gcgtacactg gcgcttcttt
    25681 atcgccagtt gggttgtaat ccagcgccag caataccgga gaacgttctg aaccgcgtcc
    25741 gacggtgtgc agccccatat aaccttgatc acgcagatct tcacctttgg tgatccgata
    25801 agtcacacga tcgcccgcga cgttgctgat cagatcaaca gcacgctgtg ccagttgcga
    25861 tggtcccaat tcttctgccg gtgcgttgat ggtgtcacgc acccagtcga tgatcatcag
    25921 gcggttatcc agttcctggc gctgggcatc gtccagatcc ggccactcca ctttacgcgt
    25981 gcctttcggg gctttgtaac cttgccagaa tgcccagcag cgatccgcat cccagccttc
    26041 accgcttaac tgaacatgct tgatgcccag accgtcaatc ttgcgcgccg cacgctggat
    26101 cagccccaga tcgtctgccc cgttcaaatg cagggtaatg ccgtcattat taatgctgta
    26161 agttgctttt tctccccagc gcgcgtcggc aggttgggta gagagggtaa tcttcatcgc
    26221 ttctgtcatt ttagttatcc ttattcgttt aacagcaaaa cgggctaccc ccaggcagcc
    26281 cgttcattca ctcggcttcg tccagccaga ctaacaaaat cgcttcgagg attttctcgt
    26341 tggatgcctg cgggtcgtcg tcgaaatctt ccagatcgca aatccactga tgcatatcgg
    26401 tgaatcgaac cgttttcgga tcaagatcgg gatacgcatc gtacagtgct tcgccaattt
    26461 cgcggctatc ggtccactta agtcccatac taacctctgt taatgctcac gcgcatggtt
    26521 gatagtgtaa cgcgggattt cgaccactaa atcttcgtcg gtaacgcgcg cctggcagct
    26581 taaacggctt tccggctcca gcccccaggc tttgtccagc atgtcgtctt cctgctctga
    26641 gctttccggc agtgagtcaa aaccttcacg aacgatgcag tggcaggtgg tgcaagcaca
    26701 ggatttttca caggcgtgtt caatctcgat accgttacgc agagctgcgt cgagaatggt
    26761 ttcaccgcta ttagcttcca gaacagcgcc atcagggcag agatcctgat gaggcaaaat
    26821 aacaatcttt ggcatattaa acctcgtcca cggaatggcc tttcagcgca cgacgaaccg
    26881 actggtccat gcggcgagcg gcgaaatcct gggtttgttt gtctacgttt ttaatcgctt
    26941 gttcgatggc gtcaacgtca ttgccctgcg ccacttcact caggtgagcg gcagcatcgt
    27001 caatgacctg acgttctgcg gcgcttaaca gcgcggcatc agcagccagc gcgccgtgca
    27061 gactttccag cacacgcgcc gcttctactt tttgttctgc cagcattcgg gcttttacgt
    27121 cctgctcggc atagctcatt gagtctttga tcatcgaagc gatttcgcta tcggtcagac
    27181 cgtaagacgg tttgacctga atagacgcct caacgccggt ggatttctcc atcgccgtca
    27241 cgctcaaaag accgtcggca tcgacctgga acgtcacgcg aatatgcgca ccgccagccg
    27301 gtagcgccgg aataccacgc agcgcaaaac gcgccagtga gcggcagtcc tgcaccagtt
    27361 cgcgctcacc ctgcattaca tggatagaca tcgccgtctg accatcttta aaggtggtga
    27421 aatcctgagc gcgggccacc ggaatagtgg tattacgcgg aatcactttc tccaccagac
    27481 cgcccatcgt ttcgaggccc agcgacagcg ggatcacatc cagcaacagc atttcgctgt
    27541 ccggcttgtt acccaccaga atatccgcct gaatcgccgc gccaatagcg acgactttat
    27601 ccgggtcgat ggaagtcagc ggtggacgac cgaaaaattc gcctacccgt tcacgcacca
    27661 gcggcacgcg agtagaaccg ccaaccatca ccacttccag cacttcagaa gcctctacgc
    27721 cagcgtcttt cagcgcgcga cgacaggcca gtaaggttcg ttttaccagt ggcgcgatca
    27781 gttcattgaa ttgttcacgg ctgatttcgc cctgccagcc cgcaacattc acggtcacag
    27841 agtccgcatc gctcagcgcg attttggctg caatggcggc atccagcagt tcacgctgaa
    27901 cgcggttatc gctacgatca ggaatgcccg cctgctcgcg aatgtaatcc gccagcagat
    27961 ggtcgaaatc atcgccgccg agcgcggaat caccgccggt tgccatcact tcaaacacgc
    28021 cgcgacttaa gcgcagaatg gaaatatcaa acgtcccgcc accgaggtca taaacggcga
    28081 tcacgccttc ctgaccggaa tccagcccgt aggcgatagc cgcagcggtc ggttcgttaa
    28141 gtaagcgcag gacgtgaagg cccgccagac gcgccgcgtc tttggtgccc tgacgctggg
    28201 catcgtcaaa gtacgccgga acggtgataa ctacaccatc cagctcgcct gccagggctt
    28261 cagttgcccg cgccaccagt gctttgagga tgtccgcaga aacgcgcacc gggttcagca
    28321 gccccgccgc cgtttcaatc atcggcaggc cgttttcgct ggcctggaat tgataaggca
    28381 gatgcggata gcgttgctgg atatcagcca gcgagcgccc catcagacgt ttaacagaac
    28441 taatggtgtt ggcggtgtca agcgctgcgt tagtacgcgc gtcataaccc accgaatgcc
    28501 cttgctgttg atagtgaaca acagatggca gcaggtgacg gccttcatgg tcggctaacg
    28561 tttcggcctg accgctgcgc actgtcgcca ccagcgagtt ggttgtgccc aggtcaatac
    28621 cggccgccag acgacgctga tgcggcgcag cactcaaacc aggttcacta atttgtaata
    28681 aggccatgtt tagcttccag aaattaaaaa tcgagcagtt tttcttcgag ttgttcggca
    28741 ctgcttcgca gtttatcgag aaaacgcagc ttacgcacgg tatccgccgc cgcggcccac
    28801 gtctcgttgt ctaactgttc aaccatcaac tgatggcggg tatcaaacat ctttttcaca
    28861 cgtttgataa agctttccag ccgcgcttca tctttcgcct gttcgatctc gtccagctct
    28921 tcgcgcagct ccagctgttc cataagaaac gcggtgtcgc gcacagtatg ctgctcgctg
    28981 gcgagatcaa aaccgtgcaa agaaagcaaa tattccgcgc gcattaacgg atgacgcagc
    29041 gtttgccatg cctggttaat ggttgcagat tgctgtacgg cggcgagttg ttccgcctgg
    29101 cttccgctgg cgaatttatc aggatgatac tgacgttgta gatcctgaaa acgcaggctc
    29161 agtgcctggg tatcgagttg ataacgggca ggcaagccaa agagggtgaa gtaatccata
    29221 acattctcag ggctgcgatc cgccgcgata acggcgaatc aggtaaaaca aaccccacgc
    29281 gcaggcgacc acggtggggt tatcggtatg cgcatcaaac gtggaagctt tcgccgcaac
    29341 cacactcatc tttaacgttc gggttggtga atttaaaccc ttcgttcagg ccttctttta
    29401 cgaagtccag ctgcgtaccg tccagaaatt gcaggctttt gccatcgacc acgactttca
    29461 cgcctttgtc ttcaaacacg atgtcttccg gcgtcggttc gtcaacaaat tccagtacat
    29521 aagccatacc tgaacatccg gaggttctca cgcccagacg caggccaaac cctttgccgc
    29581 ggttagccag aaaggtattg actcgcgctg ctgcactgtc gctcagtgta atcgacataa
    29641 ccaaacctca actcttattt tgcttcacgt ttgcttttat agtccgcaat ggcggctttg
    29701 atcgcgtctt ctgccagaat agaacagtga attttcaccg gcggcagttc aagttcttca
    29761 gcaatatcgg tgtttttgat cgcctgcgct tcgtcgagag acttcccttt cacccattcg
    29821 gtgaccaggg agctggaagc gatagcagaa ccgcagccgt aagttttaaa acgcgcgtct
    29881 tcaatgatac cttcatcgtt gactttaatc tgcaacttca tcacgtcgcc acaggccggc
    29941 gcacccacca tgccgctgcc gacgttctcg tcgttgttgt cgaaggaacc cacgttacgc
    30001 ggattctcgt aatgatcgat aactttttcg ctgtaagcca ttataaattc tcctgattcc
    30061 gataccgatt aatgatgagc ccattcgatg ctgttcagat ccacgccctg cttgtacatt
    30121 tcccacagcg gagaaaggtc acgcaaacga ccgatggatt tacgaactaa ctcgatggtg
    30181 tagtcgatct cttcttcagt agtaaaacga cctaaagaga aacggataga gctatgtgcc
    30241 agctcgtcgt tcagccccag cgcgcgcagc acgtaggacg gttcgaggct tgctgacgta
    30301 caggcggaac ctgaagaaac cgcgaggtct ttcagcgcca taatcagcga ctcaccttca
    30361 acgtagttaa agctgacgtt gagaatgttc ggcgcaccgt gttcgaggtc accgttcagg
    30421 taaacttctt cgatatcttt gatgccgttc catagacggt tacgcaggcc gcgcagacgt
    30481 tccatctcgg tcgccatctc ttcttttgcg atgcgatagg cttcgcccat gccgacgatc
    30541 tggtgaacag gcagagtgcc ggaacgcata ccgcgttcgt gaccgccgcc gtgcatttgc
    30601 gcttcgatgc gtacgcgcgg tttacgacgc acatacagcg caccgatacc tttcgggcca
    30661 tagattttgt gaccggagaa agacatcagg tcaactttca actggctcag gtcgataggc
    30721 agtttaccca cgctctgggt tgcatcaacg tgatagataa tgccacgagc acggcacatt
    30781 tcgccgatag ccgcgatatc ctgcaccacg ccgatttcgt tatttacgtg catgatggac
    30841 acgaggatgg tgtcgtcacg catcgctgct tcaagttctt tcaggtcgat aatgccgtta
    30901 cgctgcggtg ccaggtaggt gacttcaaaa ccttcgcgct ccagctgacg gcaggtatcc
    30961 agtaccgctt tgtgttcggt tttgctggtg atgatgtgct tgcctttttt ctgataaaag
    31021 ttggctgcac ctttgatcgc taggttgtca gattcggttg caccagaggt aaagacgatt
    31081 tcacgcggat cagcgccgac cagatcggca atctgattac gggcgatatc taccgcttct
    31141 tcagcctgcc agccgaaacg gtgagaacgg gaggccgggt taccaaaggt tccgtccatc
    31201 gtcataaact gcatcatttt ctcggcaaca cgcgggtcca ccggcgtggt tgcggagtag
    31261 tcgagataaa tcggtaattt cattgctcta taaactccgt acatcactca atgcaaggaa
    31321 tcaggctacc ggctggatgt acgaccgtgt ttacggagta tttagcactc cggcctgatt
    31381 ctgaattctt tttattaagc gcgtaactta acgtcgatcg cgtcttgtgt gcgggtgcgt
    31441 ggcgcgtcgt gagtatgctg acgaccagac acatccagca cttcctggtt attaaccagt
    31501 tcgcctaaag taatgttgtt gagaaaaccg gtgagacggt cgctcaaatc acgccacagc
    31561 gcgtgggtca ggcatttatc gccgccctgg cagccgcctt taccctgaca acgggtggca
    31621 tctacagatt cgtcaacggc gctaattact tcgccaacgg cgatgctgct ggcatctttg
    31681 cctaacagat aaccaccgcc tggtccacgt acgctggaaa ccagaccatt tttacgcaga
    31741 cgggaaaaca gttgttccag ataagaaagg gaaattccct gacgttcgga aatatcagcc
    31801 aacggtaccg ggcccgcttc agagttgagc gcaacgtcaa gcattgcggt cacggcatag
    31861 cgccctttag atgtcagtct catgtcttac ttcacctcaa actcgcccct gcccggggtt
    31921 ttttattgta aaagtggggg tattgcatag cagggtcaag tctgacattc ccgagtaaat
    31981 tggtcaacta tttacttgac tgatttagtc gggtatttaa ccttcagtgc cattttttta
    32041 tcgtggcgtt tgtacctgtt gtcggatgcg gtgtgaacac cttatccgac ctacggttct
    32101 gtacattgta ggcctgataa gacgcgttaa gcgtcgcatc aggcaaccac tgttattcgg
    32161 ccttgttacc tttattctgc tgctcaatag aagccagaat cccgcgcagg atattcaatt
    32221 cctggctttc cgggcgcgca cgggtaaaca gacggcgcaa tttattcatc acctgccccg
    32281 gatggttttc acggataaaa ccggttgcca gcagcgtttg ttccagatga ccataaaaac
    32341 gctccagatc atcgaccagc ggatacggcg tctcttcatg ctcgacctgc tcgccatttt
    32401 cctgagttgc cagccaggcc atgcgcactt cataggcaat aacctgaacc gccatcgcca
    32461 ggttcagcga gctgtattcc gggttagccg caatcgcgac atgataatgg catttctgca
    32521 actcttcatt ggtcaggccg acacgttcgc gaccaaaaac cagcgccacc ggggtatttg
    32581 ccgcttcagc aacgcttttc aaaccgcatt cgcgcgggtc gagcatcggc cacggcagcg
    32641 tgcgggaacg tgcgctggtg cccaccacca ggctacaacc ggctaacgct tcgtcgagcg
    32701 tatcgacgat gtgagcatta ccaatcacat cgctggcccc cgccgccagg gcaatcgcct
    32761 gggagtcagg tttcaccagt ggattaacca gccacagatt ggttaatccc attgttttca
    32821 tggcacgggc aacagaaccc atattgccgg tgtgtgacgt ctccaccagc acaattcgaa
    32881 tattttgcag cattgtcttt cttcgtctaa agattattcg cgcatcttat cataaaacga
    32941 agacagatgc cgatctcgct gctatactct gcgccgtttt cccgttcttt aacatccagt
    33001 gagagagacc gatgcatccg atgctgaaca tcgccgtgcg cgcagcgcgc aaggcgggta
    33061 atttaattgc caaaaactat gaaaccccgg acgctgtaga agcgagccag aaaggcagta
    33121 acgatttcgt gaccaacgta gataaagctg ccgaagcggt gattatcgac acgattcgta
    33181 aatcttaccc acagcacacc atcatcaccg aagaaagcgg tgaacttgaa ggtactgatc
    33241 aggatgttca atgggttatt gatccactgg atggcactac caactttatc aaacgtctgc
    33301 cgcacttcgc ggtatctatc gccgttcgta tcaaaggccg caccgaagtt gctgtggtat
    33361 acgatcctat gcgtaacgaa ctgttcaccg ccactcgcgg tcagggcgca cagctgaacg
    33421 gctaccgtct gcgcggcagc accgctcgcg atctcgacgg tactattctg gcgaccggct
    33481 tcccgttcaa agcaaaacag tacgccacta cctacatcaa catcgtcggc aaactgttca
    33541 acgaatgtgc agacttccgt cgtaccggtt ctgcggcgct ggatctggct tacgtcgctg
    33601 cgggtcgcgt tgacggtttc tttgaaatcg gtctgcgccc gtgggacttc gccgcaggcg
    33661 agctgctggt tcgtgaagcg ggcggcatcg tcagcgactt caccggtggt cataactaca
    33721 tgctgaccgg taacatcgtt gctggtaacc cgcgcgttgt taaagccatg ctggcgaaca
    33781 tgcgtgacga gttaagcgac gctctgaagc gttaatgact caggcgggtg atatcaatca
    33841 cccgcccccg cctttcaggc gctattccga aatacttcct caccgcttta ctttctttcg
    33901 tcactctccc accatctttc cccgattaat ggatgataaa gaagtcgcaa ggaaaggtta
    33961 tggcactgcc agtgaacaaa cgcgttccca aaattctgtt tattctcttt gttgttgcct
    34021 tctgcgttta tttagtgccg cgcgttgcca tcaacttctt ctattatccc gacgataaaa
    34081 tttacggtcc cgatccctgg tcggcggaat ccgtcgaatt tacggctaag gacggtactc
    34141 gtctgcaagg ctggtttatc ccttcttcga cgggccctgc tgacaacgcc atcgcaacca
    34201 tcattcatgc tcacggcaat gccggaaata tgtccgccca ctggccgctg gtcagttggt
    34261 tacccgagcg taatttcaac gtttttatgt ttgattatcg cgggtttggt aaatcaaaag
    34321 gcacgccgtc ccaggccgga ttgctggacg atacgcaaag tgccatcaat gtggtgcgcc
    34381 atcgcagtga tgtaaaccca caacgtctgg tgctgttcgg gcagagtatt ggcggggcga
    34441 atattctgga tgttattggt cggggtgatc gtgaaggcat acgtgcggtg atcctcgact
    34501 ccacatttgc ctcttatgca accatcgcca accagatgat ccccggcagt ggctacttac
    34561 ttgatgagag ttacagcggc gaaaattata tcgccagcgt cagcccgatc ccgcttttac
    34621 tcattcacgg taaagctgat cacgttatcc catggcagca cagcgaaaag ttgtatagcc
    34681 tggcaaaaga gccaaaacgg ttgatactaa tcccggatgg cgaacacatt gatgcttttt
    34741 ccgatcgtca cggcgatgtt tatcgcgaac agatggtgga ctttatcctt agcgcgttga
    34801 atccacagaa ctaacccatg atcgctagca cgataatcat tcacaaaacc accttaagac
    34861 atgctaatcc actggtcaga acagtttaag atgagaaaaa ttctgtgacg cttgccaaca
    34921 tttctgatga ttagcattcc cttcgccatt tccctgagca aactttagct attcttatca
    34981 attatgctta tgggagatct acagatgatg cctacgcttg ctccaccatc tgtcctttcg
    35041 gctccccagc gccgctgcca gatcttgctg acgctctttc agccggggtt gaccgccacc
    35101 acggcaactt tcagcgagct taatggtgtg gatgatgata ttgccagtct tgatatcagc
    35161 gaaacaggac aggagatcct gcgctatcat caactcacac tgacggctgg ttatgacggt
    35221 agctaccggg ttgaaggtac agtgcttaac caacgtttgt gtttatttca ctggctacga
    35281 cgtggtttcc gtctgtgtcc atcatttatt accagccatt tcacccccgc cctgaagagt
    35341 gaactgaagc ggcgcggaat tgcgcgtaac ttttacgacg ataccaatct acaagcgtta
    35401 gtgaatctct gctcccgacg actgcaaaaa cgctttgaaa cgcgcgatat tcatttcctg
    35461 tgtctgtatc tgcaatattg tttgctgcaa caccacgctg gaattacgcc tcagtttaat
    35521 ccgctccaac gtcgctgggc ggaatcctgc cttgaatttc aggtagcgca ggaaattgga
    35581 cgccactggc agcgtcgggc gctccagcct gtaccgcctg atgagccact gtttatggca
    35641 ttactttttt ccatgttgcg ggttcccgat ccattgcgtg atgcgcatca gcgagacaga
    35701 caattgcgtc agtctatcaa acgtctggta aaccattttc gtgagctggg aaatgttcgt
    35761 ttttatgatg aacaggggtt atgcgatcag ctttataccc acctcgccca ggcgttaaat
    35821 cgcagtttgt ttgccatcgg tattgataat accctgccgg aagagttcgc cagactgtac
    35881 ccacgcctgg tgcgcaccac ccgcgcggcg ctggccggat ttgaaagtga atacggcgtc
    35941 catctttctg atgaggaaag tggtctggtc gcggtgattt tcggtgcctg gctaatgcag
    36001 gaaaacgatc tgcatgaaaa acagattatt ctactgaccg ggaatgatag cgagcgagaa
    36061 gcgcagattg agcagcagtt acgcgaacta acgttactgc cgctcaacat taagcatatg
    36121 tcggtaaagg catttttgca gacaggcgct ccgcgtggcg cggcactgat tattgcgcct
    36181 tataccatgc cattaccgct cttttcacca ccgctgatct atacggacct gacgctgaca
    36241 acacatcaac aggagcagat ccgcaaaatg ctcgaatcag catgagggaa caactttcgg
    36301 gcgcaaaaac attgccggca gcgccaccag cgccattacc cagaacacgc cgtggcccag
    36361 atattgatac aggaaaccgg cgaaaacggt catgatagcg atactgccgc ccatcgcgac
    36421 ggcagagtaa accgcctgta aacggatgac ttcgctaccc tggcgagcag caatataacg
    36481 catggcggcc aggtggcaga ccgtgaaggt gccgcaatgc agaatttgca ccactatcaa
    36541 ccacggcaac gccgtagttg ctcccataat gccccagcgc actacgccgc aaatcgccga
    36601 gatcaacagc atatcgcgtg cactacaacg gcggaaaagt ttattactca gcgcaaagat
    36661 aatgacttcc gccaccacgc ccagcgacca caaatacccc accgccgagg ccgagtagcc
    36721 agctgcctgc cagtaaatgg cgctaaaacc gtaataggcc gcatgtgccc cctgcaataa
    36781 acaaacgcag gccagaaagc gccagttctg gcgaaccagc gccaaccacg ctgaccaacc
    36841 ggtgctctcc tgctggcggc ttgccccttg tggctgaatc gtcggacgga tgagaaagcc
    36901 gagcagcatg gatgccacgc ccaacgtcaa cagcgcgagg atcacccgat aatcaaacat
    36961 agtgaccagt ttgcccgtca gcgccgagcc aatgacaaac gccaccgagc cccacagtcg
    37021 cactttgccg taatcaagcg ggaactgctt ttgccacgta ttcgccagtg catcggtcaa
    37081 cggtaccagc ggtgagaaaa agaggttaaa gccaatcatc accagcatca gccacgctac
    37141 gtgcgccccc gcccagaagg cgacagcaaa gagaagtgtc agcagtgcca gcacgcgcaa
    37201 ggcggaaatc aggcgggaag gatcgctgac gcggggcgcg atgagcaaac tcccgagaaa
    37261 acgggcaacc agacctgccc ccaataacag gccgatggtt tctggcgtta aaccaatccc
    37321 tttaagccag acgctccaga aaggtagaaa aatgccgtaa ctaaaaaagt atgtgaaata
    37381 gccgagcgcc aaccagcgcg tggattgcaa aaccatgagt ccctcccgtc aggaggcgtt
    37441 agtctggggt taatcgaatg ttggtgcaag ttgaaaaata agaatattcg agcctgcttc
    37501 ccgatgtctg taacgccagt tcacaagcac taaaaaactg agatccgcga cgcatgttgc
    37561 aaaaataatg cgtaattatg ccgttacgct tgccaaacgt tcctgcacaa tggcgatgaa
    37621 atcgcgtaat gccggcttca tctccccttt cttccacgcc atcagtaaag caatggaagg
    37681 cacatttccg gcaatgggac gaaaaacaac ctgtccggta ttaaaattat tcatataacc
    37741 gggtatcaaa gtgacgccca gccccatgcc caccagattc atggtcacca gaatattcgt
    37801 tgccacctgg acgatatttg gctggctgtt ttcttgcgca aaccacgctt taacgatcgg
    37861 cgcaagcgaa ccggaatacg ccggatcggt actgacgaaa ttcacgccat ccagttgcgc
    37921 ggcggtgatc tctttttcat gtgctaaagg gtgatcaacc ggtaacacaa ccactaatgg
    37981 ttcgtcaaaa agctccagat aatcaatctc cgggctataa acaggatggc gcattaagcc
    38041 gacgtcgagt tcaccacggc gaattttttc ctcctgttgc gtggtgatta aactcaccag
    38101 ctcaatcaag gtgtctggct gtctgagacg aaacatcggt aatacttttg gcagtaaatt
    38161 cacttccgcc gatggcacaa agccaatggt taattgtctg tcttcctgaa caattttccg
    38221 cgcccgtaat ttggcatttt ccgcttgttc gaggattgcc agcgcgtcct ggagaaaaca
    38281 ttctcccgcc gctgtcaacg cgactttgcg cttatccctc accagtaacg gaacaccgac
    38341 acagttttca agatcgcgga tctggctgct taacgaaggc tgtgaggtat gcagtttttc
    38401 cgccgcacgg gtaaagttca gtgcctgcgc cactgcgacg aaatagcgta aatgccgtag
    38461 ttccatcacc ttccccttgt tatcgaaaaa acgtctcagc tggtagaaat taactatttc
    38521 acatattagc aaccaaccag caacatcctt atggcacaaa aatagaaggt caatacatct
    38581 tatctttcag gattaaaaaa tatgaccaca ccctcagatt tgaacattta ccaactgatt
    38641 gacacccaaa atggtcgggt cactccgcgt atttataccg acccggatat ttaccaactg
    38701 gagcttgagc gtattttcgg tcgttgctgg ctatttctcg cccacgaaag ccagatccca
    38761 aaacccggtg atttctttaa cacctacatg ggagaagatg cggttgtcgt agtgcgtcag
    38821 aaagacggca gcatcaaggc gtttctcaac caatgccgcc accgggccat gcgtgtgagt
    38881 tatgcagatt gcggcaacac tcgcgccttt acctgcccgt atcacggctg gtcttatggc
    38941 attaacggcg agttgatcga tgtaccgctg gaacctcgcg cctacccaca agggttgtgt
    39001 aaatcccact ggggactaaa cgaagttcct tgtgtggaga gttataaagg gctgattttt
    39061 ggcaactggg ataccagcgc accgggcctg cgtgattacc tgggtgacat tgcctggtat
    39121 ctggatggca tgctggatcg tcgcgaaggc ggcaccgaaa ttgtcggcgg cgtacaaaag
    39181 tgggtgatca actgtaactg gaaattcccg gcagagcagt tcgccagtga ccagtatcat
    39241 gctctgttca gccatgcttc tgccgttcag gtattagggg cgaaagatga tggcagcgat
    39301 aagcgcctcg gtgatggaca aaccgcccgc ccggtgtggg aaaccgccaa agatgcgctg
    39361 caatttggtc aggacggtca cggtagcggt ttcttcttta ctgaaaaacc ggatgctaat
    39421 gtctgggtcg atggcgcagt ttcaagctat taccgcgaaa cctatgccga agcagaacaa
    39481 cgtttaggtg aagttcgcgc cctgcgcctg gcgggtcata acaatatatt ccccacgctt
    39541 tcatggctaa acggcactgc cacactccgc gtctggcatc cgcgcggccc tgatcaagtt
    39601 gaagtgtggg cgttctgtat tactgacaaa gccgcctccg atgaagttaa agccgctttt
    39661 gaaaacagcg ccactcgtgc ttttggtcct gctggttttc tcgagcagga tgactcggag
    39721 aactggtgtg aaatccagaa attgctgaaa ggtcaccgcg cccgcaacag caaactgtgt
    39781 ctggaaatgg ggcttggtca ggaaaagcgt cgcgacgacg gcattccagg cattactaac
    39841 tatatcttct cagaaacggc cgctcgtgga atgtaccaac gctgggccga tcttctgagt
    39901 agcgaaagct ggcaggaagt gctcgataaa accgccgctt accagcagga ggtgatgaaa
    39961 tgagtgcgca agtttcacta gagttacatc accgcattag ccagtttctc tttcacgaag
    40021 ccagcttact ggacgactgg aaatttcgtg actggctggc gcagctcgac gaagagattc
    40081 gttacaccat gcgcaccaca gttaacgcgc aaacacgcga ccgccgcaaa ggcgtccagc
    40141 caccgacaac ctggattttt aatgacacca aagaccagct ggagcggcga atcgcccgtc
    40201 tggaaacggg catggcatgg gcagaagagc cgccgtcacg cacccgccac ttaatcagca
    40261 actgccagat aagcgaaacc gacatcccaa acgtatttgc tgttcgggtg aattatctgc
    40321 tttatcgggc acaaaaagag cgcgatgaaa cgttctatgt cgggactcgt tttgacaaag
    40381 ttcgccgtct ggaagatgac aactggcgct tgctggaacg ggatatcgtc ctggatcaag
    40441 cggtaatcac ttcccataac ctgagtgtac tgttctgatg aatcgaattt atgcgtgtcc
    40501 cgttgcggat gtgccggagg gtgaggctct ccggatcgat acctcgcccg tcatcgccct
    40561 gttcaacgtt ggcggcgagt tttatgccat taacgatcgt tgcagccatg gtaatgcgtc
    40621 aatgtcagaa gggtatctgg aagatgacgc cacggtggag tgcccgctac acgccgccag
    40681 tttttgcctg aaaacgggga aagcgttatg cctgcccgcc accgatccgc tcaccactta
    40741 tccagtacac gttgaaggtg gtgacatttt catcgactta ccggaggcgc agccatgagc
    40801 gatctgcata acgagtccat ttttattacc ggcggcggat cgggattagg gctggcgctg
    40861 gtcgagcgat ttatcgaaga aggcgcgcag gttgccacgc tggaactgtc ggcggcaaaa
    40921 gtcgccagtc tgcgtcagcg atttggcgaa catattctgg cggtggaagg taacgtgacc
    40981 tgttatgccg attatcaacg cgcggtcgat cagatcctga ctcgttccgg caagctggat
    41041 tgttttatcg gcaatgcagg catctgggat cacaatgcct cactggttaa tactcccgca
    41101 gagacgctcg aaaccggctt ccacgagctg tttaacgtca atgttctcgg ttacctgctg
    41161 ggcgcaaaag cctgcgctcc ggcgttaatc gccagtgaag gcagcatgat tttcacactg
    41221 tcaaatgccg cctggtatcc tggcggcggt ggcccgctgt acaccgccag taaacatgcc
    41281 gcaaccggac ttattcgcca actggcttat gaactggcac cgaaagtgcg ggtgaatggc
    41341 gtcggcccgt gtggtatggc cagcgacctg cgcggcccac aggcgctcgg gcaaagtgaa
    41401 acctcgataa tgcagtctct gacgccggag aaaattgccg ccattttacc gctgcaattt
    41461 ttcccgcaac cggcggattt tacgggtccg tatgtgatgt tggcatcgcg gcgcaataat
    41521 cgcgcattaa gcggtgtgat gatcaacgct gatgcgggtt tagcgattcg cggcattcgc
    41581 cacgtagcgg ctgggctgga tctttaagga agcacgatga aagaaaaaac catcattatt
    41641 gtcggtggcg ggcaagcggc ggcaatggct gcggcctcgc tacgccagca agggttcacc
    41701 ggtgagctgc atctgttttc cgatgagcga catcttcctt atgaacgacc tccactctcg
    41761 aaatccatgt tgctggaaga ttccccgcag ttacagcagg tgttacccgc taactggtgg
    41821 caggaaaaca atgttcatct gcattccggt gtaaccatca aaacgctggg tcgcgacaca
    41881 cgagagttag tgttaaccaa cggcgaaagc tggcactggg atcagctttt tatagcaacc
    41941 ggcgcggcag ctcgaccgct gccgttgctt gatgcactgg gagaacgctg ctttacccta
    42001 cgccatgccg gtgatgccgc cagactgcga gaagttctgc agcccgaacg gtcagtcgtg
    42061 attgtcggtg ccggaactat tggtctggaa ctggctgcca gcgccacgca gcgcagatgt
    42121 aaggtgacag tgattgaact ggcggcaacc gtcatgggcc gtaatgcacc accgcccgtg
    42181 caacgctatc ttttacagcg ccaccagcag gctggtgtgc gcattctgct caataatgcc
    42241 attgaacatg tggtcgatgg tgaaaaagta gaactgacgc tgcaaagtgg ggaaacgctt
    42301 caggctgatg tggtgattta cggtattggt atcagcgcca acgagcaact ggctcgcgag
    42361 gccaaccttg atactgccaa tggcattgtc attgatgagg cttgccgcac ctgcgatccc
    42421 gcgatctttg ccggtggcga tgtggcaatc actcgtcttg ataatggtgc actacaccgc
    42481 tgcgaaagct gggaaaacgc caataaccag gcgcaaattg ccgctgccgc aatgttaggg
    42541 ctaccgctac cgctactgcc gccgccgtgg ttctggagcg atcagtacag tgataactta
    42601 cagtttattg gcgatatgcg tggcgatgac tggctttgtc gtggcaaccc ggaaactcag
    42661 aaggcgattt ggtttaatct gcaaaacggc gtgcttatcg gtgcggtcac gctgaatcag
    42721 gggcgtgaga ttcgcccaat tcgcaaatgg atccagagcg gcaaaacgtt tgatgcgaaa
    42781 ctgctgatag atgagaacat cgcgcttaaa tcactgtaac caggataatt agcgaatatc
    42841 tcaatgcctg gggcgtggcg aggtgcaaga gtgtgtatta cgtttaaatc acattatctt
    42901 gcaaagggat tggttatgaa cacactacgt tattttgatt ttggagctgc ccgccccgtt
    42961 ttgttattaa ttgcccgtat cgccgtggtc ttaattttca ttatttttgg ttttcccaaa
    43021 atgatgggct ttgacggtac ggtccaatat atggcctcgt tgggcgcgcc gatgccgatg
    43081 ctggcagcga ttattgcggt agttatggaa gtgcccgccg cgatattaat cgtgcttggc
    43141 tttttcaccc gtccgctggc ggtgctgttt attttctaca cgctgggtac ggcggtgatt
    43201 ggtcaccatt actgggatat gaccggcgat gcggttgggc caaatatgat taatttctgg
    43261 aagaatgtca gtatcgctgg cgcattcctg ctgctggcaa ttaccgggcc gggggcaatt
    43321 tctctcgatc ggcgttagga aagatgccgg atgcggcgtg aacgccttat ccggcattta
    43381 ataaattaca gccactctac ccgcaacgac atctctgaag ttgttgattc ccctggtgct
    43441 agcgcaatga gatcaccccc ttccgggcga tgatgatcat ccggcgcatg gctcatcggt
    43501 tccagacaga agaaatcaaa cgcatatcct ttatcaaacg cagggtcgga aacaaagatg
    43561 aaataacacg gtgcaggtgg cgtcgtttcc atgatgatgg cataaccttc ctgcggctgc
    43621 tcgatgcggg cctgaccatt ccatccggca aaaccattgt tcacccactg gcgcggcaac
    43681 ggcgcgggct ggttaaaatc cagttcctgc ggtagctgct cgcaaaactc acccgccagc
    43741 cactgctccc gctccagcca gtaaccgctc gcctgtgcct gaatccgcgt ttgcggcgac
    43801 aacggaaaat aaggatgcca gccggtacca aatggcagcg tctctgcccc ttgattggtg
    43861 acagagagcg tcaccgtcag cgtatccgcc gttaaatgaa acgcctgact tacccgatag
    43921 tgatagacac cgctgcgatg ttcatacacc agacacaaac tatcatcgct gtgcgagaca
    43981 cattgccact cgcccagcca gccatcgccg tgtagatagt gcgcatccca ctcaacattc
    44041 ggttgcagtt gatactcacg cccctgccag acaaaacgat tgccgctcac ccggtttgca
    44101 aatggcacca gcggaaaaca tgaggcatcg gttgccacac cgcttttttt accaggacgt
    44161 aagagcggcg tcgtatcgcg ccagaagcct tcgattacgc cgccctggtc agaaacgtcc
    44221 agctttagcg acccgtggga taaggtatag atggtcatcc gctaactcct taatccggga
    44281 agttaatcac aactttcccg catttgccgc tcgccatcag cgcataggca tcacctgcct
    44341 gttccagcga gaagcgatgg gtaatggcgt tacgcggcca cagcttccag tccgtcagat
    44401 catgggcgca tttttccata tggaacagac tggtcaccca ggagccgata atccgccgtt
    44461 gatggtgcat cagatcggcg ctgacctcga attccacttt tccggtttca ccaatgtaaa
    44521 ccacccgtcc ccagtcagcg gtggattgca gtgccagcaa gcgacctgcg gcattaccgg
    44581 aacaatcgag cgcaacatcc gcgccaccgt gggtgagttc ggcgataatc tgcggcagac
    44641 cttcggtggt tgctaaatag ccgtgatcca tcacccctaa ctgttttgcc atcgccagac
    44701 gttccggcag catatcaacg ccgatgatcc gttttgcacc gcgacctttc gccagcatca
    44761 tcgccatcat gccgaccggc cccagaccga ccaccagcac gttatcgctg ccggaaactt
    44821 cgccgcgcaa aatcccttca taggccgtgc caacgccgca actgataaac gcaccatctt
    44881 cgtagctcag cgcatccggc aggaggatca gatctttttc ttccgccagc agatattcgg
    44941 catgaccgcc gtcacgctgc cagccgtaag ccgcttttcc tttgccagta caagagatag
    45001 gaaagccgcg gcggcagttc gggcaaaaac cacagccaga aatgtgatac accagcacgc
    45061 ggtcgccctc tttaaaatgg cggcagcctt gccccatcgc cacaatctgc ccgcacggtt
    45121 catgaccgtt gataaatccc tggtataacg gtttgtcggg tgccgctgcc gtagcgcggt
    45181 gctggtggta gatatagtgg acatcgcttc cgcaaattcc ggaagatttc attttgatca
    45241 gtacctggtt aatccctggt gtcggcaccg caacttcccg tagatcgacg gtcgaatttc
    45301 ctggtaaata agctgccagc atcgttttca taaatccctc attaacaata cgattaattt
    45361 tcatccctgc cgcacccgcg ccaggggcgt taaggtttag cgtttcgctt tactgcttcg
    45421 ctgggtcagc aagatattcg ccagcaccgc caccacgatg atgacgccgc gtaccacctg
    45481 ctggaaaaag gagttaatac cgagcagcac cagaccgtta ccgattagcg taatcaccag
    45541 cacaccaagc aatgtaccga acagggaacc gcgaccgccg gaaagtgccg taccgccgac
    45601 cacgaccgcg gcgatgacgt caaactccag accgtttgcg gcacctgcgt taccagaacc
    45661 gaggcgcgcc gccaacaaaa tgccggtcac cgccgctaat aatcccgaaa gggtaaagat
    45721 aagaatgcgc acccgacgaa cgttgatgcc gcacaactgc gccgccgtag cattaccgcc
    45781 aacggcaaaa accgagcgcc cgaaggcggt tttgcggctg atgaacacaa acagcgcaaa
    45841 caacacaatc atgatcagcg cggataccgg cacaccgaga aattgtccgc ccagccagtc
    45901 cagcacctcg ttttcgtcaa tcggcactgg cagtgcgttc gtcataaaca gccccattcc
    45961 gcgcagggcg ctccacagcc ccagtgtggc aacgaaactt ggtacgttaa acacgccgcg
    46021 cagcaccccg gcgagcgttc ccatcagcgc gcctaacagc aacaccagca gacaagcaat
    46081 cgccaacgga acttcaaatt gcagcaaaaa tgccaggcac accgagacaa aagccaccat
    46141 cggcccaacg ctgacatcaa tctcaccgga gataataatc agcgtcatcg cccaggcggc
    46201 aatcccaatg gtggcggcat cgcgcagcac gttcatctgg ttattcaatg agataaagcc
    46261 aggcgcgttc agggagaaga ccagataaag aatggcaatc accaccagca aaccgatctc
    46321 attaatatgg cgactgacaa attgtttgag cgagacgctc ttgccctgcg gcaatggtaa
    46381 tgacgaagca gacatcttca ggttcctcgg taaaattcat cagtgcacag acagaatggc
    46441 ggacatcagc tcatccacat tgaccggcga gtgaaactcc tgcgagaacg taccgtgctg
    46501 taacaacagg atgcggtcgc acaccagcgg caactcctcc acttcactgg agataaacac
    46561 cacgcttttt ccttcggcag ccagctcacg gacaatacgg taaatctgct gtttggcttc
    46621 gatatcgacg ccgcgcgttg gctcgtcgag caacaaaatc tggctggcgg catagaccca
    46681 acgaccgatc accacttttt gctgattgcc gccagaaagc gtgccgatgg gcgtttcgct
    46741 actggcggcc ttgaccgtca tccgctgcat cacctcttcg gtcaggcggc ggatggtgga
    46801 ccattgcagt acgccgttgg cgctgatttt ttgccgattg gtcagcactg tattttcgtc
    46861 aacgcccaac cagggaatga tccccgcttc tttgcggttt tctggcgtat agccaatgcc
    46921 gcgtttcagc atgtcgccgt aatcggggcg cgtgattttc tcgccgttga taacaatttc
    46981 gccctgttca tactcctcca gcccaacaat cgccttcagc aattcactgc gccctgcccc
    47041 cagcaggcca gcaatgccaa gcacttcgcc gcgacgtaac gtaaagctga tatcctccag
    47101 cttgggctta tggcgtaatg cacggacttc cagcacggcc tgatcgacaa tttcctgagg
    47161 ggctaccggc gcaatatcaa cgtgatcgcg ccctagcatt agcgacacaa tatgatgcgt
    47221 ggaggtgttt tcgagcatca catcgcccgc cacctgacca tcgcgcataa cggtggcaca
    47281 ggaggcaatg cggcgaattt cttccatccg gtggctgaca taaatcaccg ccacgcccag
    47341 tgccgacatc tttttcaccg cgctgatcac cagttcaact tccgcactgg caagcgaact
    47401 cgtaggttca tcaagaatga ccacgcgcgg ctcgcccttc atcacccgtg caatttccac
    47461 cagttgcttt tgcgccgggc ttagcgttga aacaagttgt tcaggactaa cgtcaacgcc
    47521 cagcgcctgt aagcaacgtt gggcatcctg cgccatttgc aggtaatcaa tcatgccgtt
    47581 gcggcggggc cactgaccga ggcagaggtt ttccgccacc gtcagccctt ccaccagact
    47641 taactcctga taaaccgcac gaacccccag ttcagcggca cggcgagtca acgtagcttc
    47701 gtcaccttcc agtcgcgtct cgccaatcca gatatcaccg ctatccgggc gttcactgcc
    47761 ggtaagcatt cgaatgagag tcgatttgcc cgcgccgttt ttacctaaca gcgcacgaac
    47821 ttcgccttta ttgagcgtga agttaacgtt atccagcgca acgacgccgg ggtaacgctt
    47881 atttcctgcc accacttttg ctaccgggac tgcctctgtt gccgtgaaca tagggacctc
    47941 tgcgaatcag cgattagggc agaccatcaa cgtgcgttgc cagccactgt ttaccatctt
    48001 ccgttttggt atagagatcg attggcacct gaatcacttt ttcaccgtcg gcttgtttat
    48061 tgataacctt caatgtttgc gcgagaacag cattgcccat tttcttaccg gaaatatcca
    48121 ctaccgcttt cagcacctga ttgttttcca gctcctgagc aatttcagtg gtcatatccg
    48181 aaccgaaaac agcaattttt ccggcctgat tttgattacg taccgctttt accgcgccga
    48241 gtgtcgctcc gcccgattca cccataatgg cgttgagatc cggcgtggag ataattagtt
    48301 tttcaccaac ggaaatcgct ttatctaaaa cagtcccttc ctgattagcg acaatttgcg
    48361 cgccgggaac gcgggatttt aatacttctt caaatccttt acgtcgctgc acacaaactt
    48421 caaaggcttc gcaattgatg acggcaattt tcggctggtc aattttattg gcaataaaat
    48481 aatcggcggc agcattaccc agttttttac caaattccag cggatcgccg accagatacg
    48541 ccgagacata tttatcgaca cccttttgat taatacaggt gttgtagcaa atcaccggaa
    48601 tacccgcttc actggcgcga cgaacggtac ggctactgcc attttcagac actgccgata
    48661 aaataatggc atcgacatta cgcgccacga gggtatcaac aaaggtactt tctttcgaaa
    48721 tatcaccctg ggcgttagtt tcaattaact gtacctgtac tgaagaatct tttgccgcat
    48781 cctgaacgcc ttgacgcact ccagcgtaat atccctgagt atcaaggtat attgcgccaa
    48841 tggtcatttc tttttccgca gccctggcaa ataaagcgct acctaatagc gtagccatta
    48901 ataatagatt acgggttgtt ctcatttttt taggcataga gcctcctgta gggtttttat
    48961 taacaacggc ttattctaat tattttgtga tgagcggcag cgcgtgccgc tcaaatatta
    49021 cagttaatag ggtttagtga acgtagttaa aaataaaagg catcacagtg gtgaataatg
    49081 ccgctttcgg ccaggccgga ttaatcgccg tcaattcagc gcgagcagac tcatattgcg
    49141 caacctcccc gagtcccgcg gacgccagca tcgccaccat caggcatttt tctttatgct
    49201 gctgttgtaa atcgccatac agcgataaca ggtcaggttg tgaaacagca aagaaatccg
    49261 cctcaatact ggttttcgcc atctcttgcg cccactgttt catttcacta aacagttgct
    49321 gtgcggtgtg ttgttcgccc agcagtcgca gcgccattcc ttgccagaag agataatcaa
    49381 ccggctgatc gttgtaataa ctgtggatgt taatggtgcg atcgccggtc gccgccagac
    49441 gtaaacaacg catcgcttca gtttcatcgc cctgcgcgtt ggcgcatatc gcctgccaga
    49501 accagatgtc gttatcagtt tgccccggta aacggccttc gcttaaattc tccggataat
    49561 gcagcgcggc atgaagcagt tcgctggcct gctgcggctg tctggcatca aggtgctgcc
    49621 aggcgcgtaa taactggttg aggataaact gactggtgac cttcccttcc ccgccttccc
    49681 acgggtggaa tttgcgcgtg gcgagaatgt ccgccgcttt gtctgcctga cccgtgagat
    49741 gccacaaatt gagcaactct gctgtcatgt cgtcgcgttt cagcgcaatt tccagattat
    49801 tttccagacg cgccagtcgt ttctccggtg tggctccgct taacttatca agcaaatccc
    49861 gttcgaaaag cagacgtgca tcctgcggcg caagctgata agcattatca agataacgcg
    49921 cggccagctc ataatcgtgt tgcttattcc acgcatggat cgctaacccg cgccagccgt
    49981 cggcaaactc cggcgacatc tctacgcaac gttgccataa ggtaatggct ttgttgtagc
    50041 tacgcttgtt gtagtagaag caggccagta aatgacgggc aaaccagcac tcttcaatac
    50101 tctccagcgc cgccacttct tccagcgtat tcgggaaacg gacaaactgc gggaagacat
    50161 caatggcttt tgcgaccagt tcgccacgtt cggctttcgg cagcaagctg gcttgcaggt
    50221 aaagcggcag cgtgcgctgg cagtccagtg cgttcagcat ctctgccgcc agggtgggca
    50281 ttccccagtt aatcagttgc ccggcggtca tcagggcgtt aacgtcgcgt ccctgacaca
    50341 gaccgcgcca ctgcgcgaga gctgattcgc tacgaccatc gaaccagttc agccaccaca
    50401 gagtggcgtt cagcggataa tcgcgcagca gtttctcgcg ctgcacacgc gcgttgtcct
    50461 gacgaccact taacaccagc agcagattat gcaggcaaag cacttcctga ttggttgggc
    50521 aggcgcgaag actttgttgg caaaaatcca gaccagcgtc gaagttacca ttacgcgccg
    50581 ccagtcgtgc caggccataa tagccaccgg ctttgctgtt gccgctccag accgcacgcc
    50641 agaaatcctc ttcggcttgt tgatattgtc cctgacgttc gtaagcactg gcgcgaatca
    50701 aactcgcctg tccgcactgc ggatttttgt tcagcgcatg tgcgcgtttc agagcctgag
    50761 tggcatacgc caccgcttgc gggaaatctg cgcggttata ttccagcatc gccagcgcca
    50821 ggttacagcg ataatccagc gggtccagcg ccacgccgcg caggtagtaa tcgaacggtg
    50881 aacggctggc gtgatgatat tgctccagat gctgaccgat aaaccaggct tcatctgtac
    50941 tggtaatgtc ttgtgctgcc agtggcgctt tggcgacgtc cggcaacggc aactcttgcg
    51001 gctgatgttc ctgataactc aggacaatat tgccgtcggc atcggagagc tcaatggtca
    51061 gcctttcagg attgataccg tgcaacacgc cctggatggc ggtcgcaggc atcagtgcca
    51121 cggcatcatc aagtaacgcg ttgcatttgc cgatttcgcg gatcgccagg cggtatccgt
    51181 tcaacggaga gatggcatac agcccccact caatcccccg ctcactacgc tggagtttta
    51241 tcaccgcatc gcgggaggca ttttgcacca tgcccaaaga atgataaggc aggaaatact
    51301 gctcaaaacg cttctcttcg taagcatcaa gccaggtaaa atcaggctgg ttatcggcaa
    51361 aaataccggt catcagttcg atatacgggc cgttattgtc ggtcagactc ttgtcccacg
    51421 cctggccgaa ttcactgtgt ccccagctcc actgtttttt acctggcgcg atatggtggt
    51481 tggcaacgtg tagcaaaccg ccatcttcat cgtgacacca cgcgccaaca aaatcgtact
    51541 gtgatttttc agccatatat gaggttggaa cgggcacatt tttatagcga gaaatgtcca
    51601 ctccggcgga gtagtccact ttgtagtaag tgccggtggc gatggggaaa gcggagacgg
    51661 cccgtttgcc gtgatcaaac accgccgtta catccggcgg gaagacgctc tgatgccctt
    51721 cccccccttt cactgccggg ttggcccacc acaagaaatg acgcggcgtg gcgttgccgt
    51781 tatagacgcg gctggcgatt tccaacgccg cccgttcagg acgcagtgtg aaacctgtca
    51841 tcacctgtaa accgtgcatc ggctccgttt cgccgaccca caccgtctgt gcgccgtctt
    51901 catgggcttc gggggtgaaa tcaacgggca taaaggtggt cgggcgatgg tgttgcggcc
    51961 agttaaactc aatcccgcca gagatccacg gtcccagcag ccccaccagc gcaggtttaa
    52021 tgacttcatt gtgatagaca aaatcgcgtt gtttcacttt atcccatgcg cgatgcacac
    52081 gaccgcccag ttccggcagg atcatcactt tgatgtagtc gttttccagc cacaccgcct
    52141 gccaggattt cagggttttc tgctcgctca gcgtatcggt cacgccgtag ggataaaccg
    52201 cgccggacga tccctgataa acgcgatttt ccaggaacat gggatgtata tcctgcggcc
    52261 cggtttcata ggtcgggatc tcaacgcgct cttgccacac ttttactgga gtcatggtgc
    52321 cctcaatatt aacaagacat actgaattaa aagatttgtg gcagtgtatt gaacaatctg
    52381 gcaatgtttt cgcggaataa tcacgcaatt aactaaacaa ggtttagtga agatgagagc
    52441 ctgcattaac aatcaacaga ttcgccacca taacaaatgc gtgattctgg aactgctgta
    52501 ccggcaaaag cgcgccaata aatcaacgct ggcccggctg gcgcaaattt cgattccggc
    52561 agtcagtaat attttgcagg aactggaaag cgaaaaacgg gtggtgaata ttgacgatga
    52621 aagccagacg cgcgggcata gtagcggtac atggctgatt gcgccggaag gtgactggac
    52681 gctgtgcctg aacgtgacgc ccaccagtat tgagtgtcag gttgctaatg cttgtttaag
    52741 tccgaaaggt gaatttgagt atttacagat tgatgcaccg acgccgcagg cgctgctgtc
    52801 cgaaatcgaa aaatgctggc atcgccaccg taaattgtgg ccggaccgca ccatcaatct
    52861 ggcgctggca atccacggtc aggttgatcc ggtgaccggc gtgtcgcaaa ccatgccgca
    52921 agcgccgtgg acaacgccgg ttgaggtgaa gtatctgctg gaagagaagc tgggcattcg
    52981 ggtgatggtc gataatgact gcgtgatgct ggcgctggcg gagaaatggc aaaataattc
    53041 gcaggaacgg gatttctgcg tgatcaacgt tgattacggc attggctcgt cgttcgtgat
    53101 taacgagcaa atttatcgcg gcagtttgta tggtagcgga cagattggtc acaccatcgt
    53161 taatccggat ggcgtcgtct gcgactgtgg acgttacggc tgcctggaaa ccgtcgcctc
    53221 gttaagtgca ttaaaaaaac aggcgcgggt atggctaaaa tcacaaccgg ttaatactca
    53281 gcttgatcct gaaaaactga caaccgcgca gttaatcgct gcctggcaga gtggagaacc
    53341 gtggatcacc agctgggttg atcgctctgc caatgccatt ggtttgagtc tgtataactt
    53401 cctcaacatc ctcaatatta atcagatttg gatgtacggt cgcagttgtg cctttggtga
    53461 gaactggctt aatactatta ttcgccagac aggatttaac ccgttcgacc gcgacgaagg
    53521 accgagcgtg aaagcgacgc aaattggctt cgggcaatta agccgcgcac aacaggtgct
    53581 ggggattggc tatttgtatg ttgaggcgca gttacgacag atttgatggc gcgataaccg
    53641 cgataacgta gaaaggcttc ccgaaggaag ccttttttca acagattaac cgtttcgctt
    53701 atgcgtaaac cgggtaacgt gcgcagatgt cgagaacttt acctttgatg cgctcgataa
    53761 cggcttcatc attgatgctg tccagcacgt cacacatcca gccagccagt tctttcgctt
    53821 ctgcttcttt gaagccgcga cgcgtaatcg ccggagtacc cacgcgaatc ccggaggtca
    53881 caaacgggct cttcggatcg ttcggtacgc tgtttttgtt gacggtgatg ttagcacggc
    53941 ccagagcggc gtctgcttct ttaccggtca ggtttttatc taccagatca accaggaaca
    54001 gatggttatc agtaccgccg gaaaccactt tgtagccgcg ttccaggaac acttctacca
    54061 tcgctttagc gtttttagcg acctgctgct ggtaagtttt gaactcaggc tccatcgctt
    54121 ctttcagagc aaccgcttta ccggcgatta cgtgcatcaa cggaccgccc tgaccaccag
    54181 ggaaaacggc agagttcagt tttttgtaca gctcttcgct accacctttc gccaggatca
    54241 ggccgccgcg cggacccgcc agggttttgt gagtggtggt agtaacaacg tgagcatgag
    54301 gaaccgggtt cgggtagacg ccagcagcaa ccaggcccgc aacgtgcgcc atatcaacga
    54361 acaggtaagc accgatgctg tcagcgattt cacgcatttt cgcccagtcc accacgccgg
    54421 aatatgcaga gaaaccaccg ataatcattt tcggcttgtg ttctttggct tgtttttcca
    54481 gatcggcgta gtcgatatga ccggtagcat cgataccgta aggaacgatg ttgtacagtt
    54541 taccggagaa gttaaccgga gaaccgtgag tcaggtgacc gccatgcgcc aggttcatac
    54601 ccagaacggt atcgcctggt tccagcagcg cggtgtagac cgcaaagtta gcctgggagc
    54661 cggagtgcgg ctggacgtta gcgtagtcag cgccgaacag ttctttcgca cgatcgatcg
    54721 ccagttgttc aacgatatca acatactcgc aaccgccgta gtagcgtttg cccggataac
    54781 cttcagcata tttgttggtc agctgagaac cctgcgcctg cattacgcgc gggctggtgt
    54841 agttttcgga ggcgatcagt tcgatgtgct cttcctgacg tactttttcc tgctccatag
    54901 cctgccacag ttcggcatca taatcggcaa tgttcatttc acgctttaac atccgcatct
    54961 cctgactcag ctaacaataa aatttttggc ctttataggc ggtcctgttg gacaacggcg
    55021 aacagtataa ccgaatcatt gtgcgataac aggtcttgac aaaggaattt acgcaaacga
    55081 ttaccttcag gctacgcaag gctttggaga ataaagagct tgcaaccgga aacggatttc
    55141 ttttcaggtt tgtgatgcaa atttttcact tcatcacatt ctttctgaaa aacaccaaag
    55201 aaccatttac attgcagggc tattttttat aagatgcatt tgagatacat caattaagat
    55261 gcaaaaaaag gaagaccata tgcttgacgc tcaaaccatc gctacagtaa aagccaccat
    55321 ccctttactg gtggaaacgg ggccaaagtt aaccgcccat ttctacgacc gtatgtttac
    55381 tcataaccca gaactcaaag aaatttttaa catgagtaac cagcgtaatg gcgatcaacg
    55441 tgaagccctg tttaacgcta ttgccgccta cgccagtaat attgaaaacc tgcctgcgct
    55501 gctgccagcg gtagaaaaaa tcgcgcagaa gcacaccagt ttccagatca aaccggaaca
    55561 gtacaacatc gtcggcgaac acctgttggc gacgctggac gaaatgttca gcccgggcca
    55621 ggaagtgctg gacgcgtggg gtaaagccta tggtgtactg gctaatgtat ttatcaatcg
    55681 cgaggcggaa atctataacg aaaacgccag caaagccggt ggttgggaag gtactcgcga
    55741 tttccgcatt gtggctaaaa caccgcgcag cgcgcttatc accagcttcg aactggagcc
    55801 ggtcgacggt ggcgcagtgg cagaataccg tccggggcaa tatctcggcg tctggctgaa
    55861 gccggaaggt ttcccgcatc aggaaattcg tcagtactct ttgactcgca aaccggatgg
    55921 caaaggctat cgtattgcgg tgaaacgcga agagggtggg caggtatcca actggttgca
    55981 caatcacgcc aatgttggcg atgtcgtgaa actggttgct ccggcaggtg atttctttat
    56041 ggctgtcgca gatgacacac cagtgacgtt aatctctgcc ggtgttggtc aaacgccaat
    56101 gctggcaatg ctcgacacgc tggcaaaagc aggccacaca gcacaagtga actggttcca
    56161 tgcggcagaa aatggcgatg ttcacgcctt tgccgatgaa gttaaggaac tggggcagtc
    56221 actgccgcgc tttaccgcgc acacctggta tcgtcagccg agcgaagccg atcgcgctaa
    56281 aggtcagttt gatagcgaag gtctgatgga tttgagcaaa caggaaggtg cgttcagcga
    56341 tccgacaatg cagttctatc tctgcggccc ggttggcttc atgcagtttg ccgcgaaaca
    56401 gttagtggat ctgggcgtga agcaggaaaa cattcattac gaatgctttg gcccgcataa
    56461 ggtgctgtga tttgatgttg ccggatggaa acatccggca acccttgacg cggtttaaat
    56521 tgccgcgtcg tcctcttcac cggtacggat gcgaatgacc cgtgccacgt caaagacgaa
    56581 gattttaccg tcaccgattt tgccggtttg cgccgtgcga ataatggtat cgacacaggt
    56641 atcgacaatg tcgtccggta cgacaatctc aattttcact ttcggcagaa aatccaccat
    56701 atactccgcg ccgcggtaca gctcggtatg gcctttctgg cgaccaaagc ctttcacttc
    56761 ggtcaccgtc atgccggtaa taccgacttc ggccagtgct tcgcggacat cgtccagctt
    56821 gaagggtttt ataatcgcat caatcttttt catgctattc cttgaaaagg tcgcctgtct
    56881 tttgatctgc taaacgtaac acataacgcc aattcattcc ttgaaatcgt ttgcatccag
    56941 ctcgtgtcgg gaaagcagtt tataaaattc tgtccggttg cgccccgcca ttctcgccgc
    57001 gtgggtgacg ttgcctttgg tgatttgcag cagcttacgc aaatagttga gttcaaactg
    57061 attacgtgcc tcaacaaagg ttggcagcgc cgtattttca ccctccagcg cctgctctac
    57121 taatgcatcg ctaataaccg gagatgaggt cagcgccacg cactgttcaa tcacgttgac
    57181 caactggcgc acattacccg gccagctcgc ggtcatcagg cgtttcatcg catcggtaga
    57241 gaacgcgcgg acaaacggtt tatgtcgctc tgccgcctgg cgcaacaggt gatttgccag
    57301 tagcggaatg tcttctgtgc gctccgccag tgccggaatt ttcaggctga caacgttgag
    57361 gcggtaatag agatcttcgc ggaattcccc gcgcgccatc gcttttggca gatcacggtg
    57421 agtggcagaa ataatccgca cattgatatc aatatcgcgg ttactgccca gcgggcgcac
    57481 tttacgctcc tgcaacacgc gcagcagttt gacctgtaac ggcgcaggca tatcgccaat
    57541 ttcatcgaga aatagcgttc cgccttccgc cgcctggaat aaaccttcgc gattgctgac
    57601 agcgccagta aacgcgccac gcgcatgacc aaacagctcc gactccagca attgctcggg
    57661 caatgcgcca cagttaatag caataaatgg tttgctgttg cgcgggctgg cgttgtggat
    57721 agcctgggcg aaaatctctt tcccggtgcc gctctgaccg ttaatcaaaa cgctgacgtc
    57781 tgattgcgcc accagccgcg cctgttccag caaacgcagc atcagcgggc tgcgggtgac
    57841 aattgcctcg cgccagcgtt catcggtggc tggcgcggat tgctccagcg catcgtcaat
    57901 tgcctgatat agcgcgtctt tgtcgacagg cttggtgagg aaactaaaaa cgccctgctg
    57961 tgttgcagca acggcatcgg ggatagaacc atgcgcagta agaataatta ctggcattcc
    58021 cggctgcact ttctggattt cagcaaatag ctgcataccg tccatttcat ccatccgcag
    58081 gtcgctgata actaaatcta ctttttcgcg attcagtacc cgtaatcctt cagcgccact
    58141 ttccgccgtg accacactgt agccttcgct ggtcaggcgc aggccaagca gtttcagcaa
    58201 tcccggatcg tcatcgacca ataataaatg cgcaggttta tggctcatca ggagtgacct
    58261 catgggtgga tggcgcgggc ttttcactct cgtgcggcgt atccggcgag aaatttccag
    58321 ccggtttgcg ggtcgagagc tggcgttcaa tatcggtcag gttttccagc ttgcgggtgg
    58381 tgagttccag ttgctgctgt agaacgtgat gttgctggcg caatgtatcc agctcgctgt
    58441 cgctggactg ctggagttta ctgtaacgtt ggcgctcttc cgccagttgc agttgcagcg
    58501 cctgaccgtc gcgccagagt tgatacagtg ggcgaacctg tgccgggatc tcggtactta
    58561 acgcttcaat acgcgcgacc agctggcggc gctcatacgg cgtaattttg gcgtcggcga
    58621 gcaaaatccc ttgtttaaag gtattttgcc agttgccgtc gtcatattgg cgagcttgct
    58681 gacgcgactg cgcaggcatt aaacgatcag cacaatccat cgcccgcagc cagtaaagcg
    58741 gattggtttc ggttgattta ccttgcagcg cccagatgtc gctacattca gtagaaagat
    58801 agtcagccag ttgataaacc ggaatttttt cttctgctgg cgtatcaatg gctggcttat
    58861 tgtgattctg cacgcaaccc agcaatgcca gacatggcag ccctgccagc cacagccgtc
    58921 ggggcaataa tcgttgaaaa atgtgtcgca tattcaccag acttaaagcc tatcccagtg
    58981 ggcgtaattg ttgcagacag tctggacatg gacagcgcgg agaaaccgga gcgtacatat
    59041 catacgtgaa gatttcgagc actgcccggg gccgaactga caaataaaat agcctgatag
    59101 gataggctgt tagcattatt tcgtgttttt cgacgacggt aattcaatgc ggaaacaaac
    59161 gtcttgcccg ctctcgtcga ccagatacag ttccccttgc atacggcgaa tacaatccct
    59221 ggcaatgctt aatcccagac cgctgccctt caccgcccct tttcgctggt ggcttccctg
    59281 aaaaaagggt tcgaagatca tggcgcgttc ctcttgcgga atgggcgtgc ctgtattgat
    59341 gacatcaata taaacccgcg caccatgtga actgctgcga aggcaaatgt taccggattc
    59401 agccccgtag tgcaccgcat tggagtaaag attatccagt acgctcatca gcagcattgg
    59461 ctccgccagg caagctgttg ctttgagatc gacgtcggta tgcatcattt tagcccgtgc
    59521 gggcaggcta tgagcagaaa ccactgtctc caccagcggt gctaattcaa cattctccag
    59581 ttccaccgca ctgtccgcct gtttacggtt gtaatcaagc agttgttcga tcagtttttg
    59641 caaattgcgg ctgctgctat caagaatgct caccacctct ttttgctctg gcgtaagcgg
    59701 cccgacaacc tggtcagcca gtaattcagt gccctcgcgc atactcgcca gtggcgtttt
    59761 taattcatga gataaatgtc ttaaaaattg atggcgttgg gattccagcc atgacaggcg
    59821 ctcacttaac caaagaatac gttgcccaac cgagcgtaac tcgctcggtc cactgaacga
    59881 aacgctattg cccagagaac gcccttcccc cagacggttg atcatgcgct cgatattttt
    59941 caccggcccg ataatcatcc gcgtgaaaag tagcaccatc accagactca ccagaaatag
    60001 caccagcgat tgccaaccaa aatattgccc acgttcggcg atttcacgct gaagttgctg
    60061 cccacgagag aacaccactg tgcgcgtggc ctgcaccatt tcggtattgg cactggcaaa
    60121 ggcttcaaga cgcgcggcgg cggcagcatc gggaccactg ttgttacact gaagttgggc
    60181 cagattgttc aaatcctgac gcaatgcctg gtaaagttta tcgtctggca gtacgcctgc
    60241 gtgggcatcg agcatttcgc tgtaacgctt gcgctggctt tgataaacct tcgcaagcgt
    60301 agggtcgtcc agcacgcaat actgacggta gctacgctcc atctccagcg ccgcgttggt
    60361 catcgcttca ctacgccggg catcaataag cgtagtgcgg ttaaccagcg ccgcctgatc
    60421 gctaagcgca ttcaggcttt gccaggcttg ccatgccagc accaacaagg gcagcagaat
    60481 tagcaaaaat gccagcatta ccagttgtcg taatgagcgg ggaaaaacgg gccagcgttt
    60541 caaggtgtta ctctcgtcag acgcgaatag cctgatgcta accgagggga agttcagata
    60601 caacaaagcc gggaattacc cggctttgtt atggaataag gcggtgccta actcgacgtt
    60661 tcgcccgatg gttgatatag ctacgctgat atcagaagtt ggacggcagg caccttgttg
    60721 tgcgtcattc gaattttatg tagcacgtcc cgaaggggct gacataagtc ggtgaatgag
    60781 ccactgctta ctattatgca gtaactgtgc caataaagaa aatagtttgg taacgtactg
    60841 attatacgtt gctttgaggg atttatggct tcttcgctat ttgaatgtaa atcagccact
    60901 gtgtcgccaa aaagagacaa cttaagataa acttattaga ttacaataaa atcaatcatt
    60961 taagtgtcgc cagaaagcga cacggtaaac cacccattgt cgtgatttac agacacaaaa
    61021 aagctcccgg agttgggagc ttatgatagt ggttggtgct taatgccgca tccgggctgc
    61081 aaaaccaatg ggctgacgac ttaccccaac tgcttacgcg cattgcggaa aatacgcatc
    61141 catgggccat cctcgcccca gttttccgga tgccaggagt tgctgacagt acggaaaaca
    61201 cgttccgggt gcggcatcat aatggtgact cgaccacttt cagtcgtgac tgccgtaata
    61261 ccgttcggtg aaccgttcgg gttagccggg taggtttcag tgactttgcc gaagttatcg
    61321 acatagcgca gtgccaccag ccctttgctt tccagtgccg ccagatgcgc cgcatcacgc
    61381 acttccacgc gcccttcacc atgagagaca gcaatcggca tttgcgagcc caccatcccc
    61441 tgcaacagca gcgacgggct ttgggtcact tcaaccaggc tgaaacgcgc ttcaaagcga
    61501 tcggaggtat tgcgcacaaa acgtggccac aactcactac ctgggatcag ttcgcgcagg
    61561 ttagacatca tctggcaacc gttacatacc cccagcgcca gcgtttgcgg acggtggaag
    61621 aaggttgcaa actcatcgcg tacacggtca ttgaacagga ttgacttcgc ccaaccttca
    61681 ccggcaccca gcacatcacc gtaggagaaa ccaccgcacg cgaccagggc gtggaaatct
    61741 tccaggcccg tgcgtccggt cagcaggtca ctcatatgca cgtcgatagc atcaaagcct
    61801 gcacggtgga aagctgccgc catttcaaca tgcgagttca cgccctgctc acgcagtacg
    61861 gcaactttcg gacgtgcgcc agtggcaata tacggtgctg ccacatcttc gttgatatcg
    61921 aacgacagtt ttacattcag gccaggatcg gcatcgttag atttcgcctg gtgctcctgg
    61981 tcagcacact ccgggttgtc acgcaggcgc tgcatctgcc aggtagtttc tgcccaccag
    62041 acacgcaacg tggtgcggct ttcgctgaat acagtctgcc cgttggcggt aatcacaaaa
    62101 cggtcaccgg aaaccgcctg ccctacataa tggacacaat cagcaagccc atgctgtgcc
    62161 agtacggact caaccgcttc acggtcagcg gcacgaacct gaatcaccgc acccagttct
    62221 tcgttaaaca acgccgccag gcgatcgtcg cccagagagg caatatccgc gttaatgccg
    62281 caatgaccag caaaggccat ttccgccagc gttaccagca ggccgccatc agagcggtcg
    62341 tggtatgcga gcagcttacg ctgtgcaacc agcgcctgaa tcgcgtcata gaagcctttc
    62401 agttgcgcga cgtcgcgtac atctgccggt ttgtcgccaa gctgacgata aacctgcgcc
    62461 agcgccgttg cgcccagcgc gttattgcct ttgcccaaat caatcagcag cagtgcgtta
    62521 tcttcggtag aaagctgtgg cgtgatggtg tgacgcacat cttccacgcg ggcaaatgca
    62581 gaaatcacca gcgacagcgg cgacgtcatt tcgcgctctt cgttaccttc ctgccagcgg
    62641 gttttcatcg acatagagtc tttacccacc gggatcgtca ggcccagcgc cggacaaagc
    62701 tcttcgccca cggctttaac ggcttcatac aggcccgcat cttcaccagg gtggcctgcc
    62761 gccgccatcc agttggcgga aagtttaatg cgtttgatat caccaatctg tgttgcggca
    62821 atattggtta acgcttcacc gaccgccaga cgggcagagg cggcgaaatc cagcagcgca
    62881 accggcgcac gctcgccaat cgccatcgct tcaccgtagt agctgtcgag gctggcggta
    62941 gtgaccgcgc agttagcgac cggcacctgc cacggcccca ccatctgatc gcgcgctacc
    63001 atgccggtta cgctgcggtc gccaatggtc accaggaagg ttttttccgc cacagtcggc
    63061 agatgcagca cacgtttcac cgcatctgca atggtgatcc cttcacgggc cagcgcgtcg
    63121 cctttcgctt tcagcgtttg tacatcgcgg gtcatcttcg gcgttttacc aagcaggacg
    63181 tccagcggca gatcgatcgg ctgattatca aaatgacgat cgtgcagaga aagatgcagt
    63241 tcttcggtcg cttcaccaat caccgcgtag ggtgcgcgtt cacgcttaca cagttcgtca
    63301 aacagcggta attgatcggc agcaatcgcc agcacgtagc gttcctggga ttcgttacac
    63361 cagatttcca gcgggctcat gcccggttcg tcgcttagaa tatcgcgcag ttcaaattta
    63421 ccgccgcgtc cgccgtcgct caccagctcc ggcatcgcgt tagaaagacc gcccgcaccg
    63481 acgtcgtgga taaacagaat tgggttagca tcaccaagct gccagcaacg gtcgatcact
    63541 tcctggcaac gacgctccat ctccgggttg tcgcgctgta cggaagcaaa gtcgaggtcg
    63601 gcatcagact gacctgacgc catggaagac gccgctccgc cgccaagacc gatgttcatt
    63661 gccgggccgc cgagaacgac cagcttcgca ccgacgttga tctcgccttt ttgtacgtga
    63721 tcggcgcgaa tgttgccgat cccgcccgcc agcatgatcg gtttgtgata accgcgcagc
    63781 tcttcgccgt tgtggctgtt cactttttct tcataagtac ggaagtagcc attcagtgcc
    63841 ggacgaccaa attcgttgtt aaacgccgcg ccgcccagcg ggccttcggt catgatgtcc
    63901 agcgcggtga caatgcgctc aggcttaccg aaatcttctt cccacggctg ttcgaagcca
    63961 ggaattcgca ggttggaaac ggagaaacca accaaacccg ctttcggctt cgcgccgcgc
    64021 ccggtggcac cttcatcgcg gatttcaccg ccggaaccgg tcgccgcgcc cggccacgga
    64081 gaaatcgccg tcgggtggtt gtgagtttcg actttcatca gaatatgcgc cggttcctga
    64141 tggaaatcgt agcggcccgt ttcgtggtca gcaaagtagc ggcccacttc agaaccttcc
    64201 attacggcgg cgttatcttt ataagcagag agaacgtgat ctggcgtggt ttcgaaagta
    64261 tttttgatca tcctgaacag cgatttcggc tgttgttcac cgtcgataat ccagtcggcg
    64321 ttaaaaattt tgtggcggca gtgctcagag ttcgcctgag cgaacatata cagctcgatg
    64381 tcgttcgggt tacgaccaag ctttgtgaaa gcatcctgca gatagtcaat ttcatcttcc
    64441 gccagagcca ggccaagacg caggttagcg tcgatcagcg cctgacggcc ctgccccagc
    64501 aaatcaacgc ttgtgaccgg agtcggttga tggtgggcaa acaactgctc tgcatcatct
    64561 aaatcaaaaa agaccgtttc catcatgcgg tcgtgcagtt cagcggtaac ctgctgccat
    64621 tgttcattgg tcagcgtacc ggcttctata tagtaagcaa cgccgcgctc aaggcggttt
    64681 acctgttgta gcccgcagtt atgggcaata tcggtcgctt tcgaagacca gggagagatg
    64741 gtgccaggac gcggggtcac cagcaggagt ttgccttgcg gggcgtggct ggcgagtgcc
    64801 ggaccatatt tcagcaggcg ttcaagttgt gcgtgctcat catcgtttaa cggcgcattg
    64861 aggtcagcaa aatggacata ctcggcgtaa atattgtgaa ccgggagcct ggcagcctga
    64921 aaacgtgcca gcagtttgtt gattcggaat gccgacagtg caggcgaacc acgcagaatt
    64981 tccatcataa gtctctcgtc ttctaagctt tcggtgtacc caaggggggg aaacgggcgt
    65041 cattataaag aatctgatgc gctgacgaaa ccgtttgcgt ggaaataaaa tcaccatcgt
    65101 gaattagcaa cgcgtgccgc caatggatgt aataagttgc catctggcgc aggtttacgc
    65161 aaaatgccgc tcatttatga gtaaaccttt cactattatt acgttttttc aagctgggac
    65221 gcgcacgaca cagagaatta actaattgaa aaaattaaag attaattatc tgttcatcgg
    65281 cattctggca ctgctgctcg cggtcgctct ctggccatcc attccctggt ttggtaaagc
    65341 cgacaaccgt atcgccgcca ttcaagcgcg gggagagttg cgtgtgagca ccattcatac
    65401 tcccctgact tataacgaaa tcaacgggaa accttttggc ctggattacg aactggcgaa
    65461 acagtttgcc gattacctcg gcgtaaaact gaaagtgacc gtgcggcaga atatcagcca
    65521 gctgtttgac gaccttgata atggtaacgc cgacctgctg gcggcaggac ttgtctataa
    65581 cagtgagcgg gtaaaaaatt atcagcctgg ccctacctat tattccgtgt cacaacaact
    65641 ggtttataaa gtgggtcagt atcgcccacg tacgctgggc aacctgacgg cggagcaact
    65701 caccgttgca ccgggtcatg tggtggttaa cgatctccag accctgaaag aaacaaaatt
    65761 cccggaatta agctggaagg tagacgacaa aaaaggctct gcggaattaa tggaagatgt
    65821 catcgaagga aaactcgatt acaccattgc tgattctgtc gccatcagcc tgtttcagcg
    65881 cgttcacccg gagctcgccg tagcgctcga tatcaccgat gaacaaccgg tgacttggtt
    65941 tagcccgtta gatggcgata ataccctttc cgccgccctg ctcgacttct tcaacgaaat
    66001 gaatgaagac ggtacgctgg cacgcattga agagaaatac ctggggcatg gcgatgattt
    66061 tgattacgtc gatacgcgca catttttacg cgccgtcgat gcggtactgc cgcagttaaa
    66121 gcccctgttt gagaaatacg ccgaagaaat tgactggcgt ttgctggccg ctattgctta
    66181 tcaggaatcg cactgggatg cacaggccac ttcaccgacg ggtgtgcgcg gcatgatgat
    66241 gttaaccaaa aataccgcgc aaagcctcgg cattacggat cgtaccgatg ccgaacagag
    66301 catcagcggt ggcgtgcgtt atttgcagga tatgatgagt aaagtgccgg aaagtgtgcc
    66361 ggagaacgag cggatctggt ttgccctcgc tgcgtacaat atgggctatg cgcatatgct
    66421 ggatgcccgc gccctgacgg caaaaaccaa agggaatcct gacagttggg ctgacgtaaa
    66481 acagcgtctg cctttactta gccagaaacc ctattacagc aagctgactt acggctacgc
    66541 tcgtggacat gaagcctacg cttatgtcga aaatattcgt aagtatcaga ttagcctggt
    66601 gggttatctg caagagaaag agaagcaggc tacagaagcg gcgatgcaac tggcgcagga
    66661 ttatccggcg gtatcgccta cggagttggg caaagagaaa tttccttttc tctcgtttct
    66721 ttcccagtcg tcatcaaact atttgaccca ttctccctct ctgctgtttt ccaggaaagg
    66781 gagtgaagag aaacaaaatt aatccgtcga ggattgcgct tttttctgcg ctttaatttc
    66841 ctggcggcgc atgcgaaaga agtcactgag caacgccgcg cactcatccg ccagtattcc
    66901 ttccgtaatt tccactcggt gattcatacc cggatgatgc agcacatcca ttaaagatcc
    66961 cgcagcgcca gttttcgcgt cacgcgcacc aaagaccacg cgaccaatgc gactgtggat
    67021 catcgctccg gcacacatta cacatggttc aagcgtgaca tacaacgtgg cgtcgatcag
    67081 acgataattt tgcatcacca gaccaccctg ccgcagggcc atgatttctg catgtgcggt
    67141 gggatcatgg cgaccaatcg ggcggttcca gccttcgccg attacccgat tgttatgcac
    67201 taataccgcg ccgaccggca cttcccgctc atcccaggca cgtttcgcca gcgtcagcgc
    67261 gtgacgcatc cagtattcgt ggctaaattc gacttcagac aaaaagaaaa ctccggttat
    67321 aaaagcgcgg cgcattatac acggacgcta tgctttactc tattccagtt gctggagttc
    67381 accgcgcggg gttactcgcc aacgatgctg acagaaataa agcagcggat tgtcctgatt
    67441 actgtcgcta tagccactgt acagccgcag cggagtgccg attttgcgct ccagttgtgc
    67501 gaccttttca tgtcccagac aacgcatcgt caatacccaa ccaccatagc cacgctgaat
    67561 ttggctggcg ataagattaa cccgcggcag ccagggcgta tcgaaataaa ccgcttcaac
    67621 cagcggctgc ggagagccgg taatcaacca gatatcagca tcggaactta acaggtaggt
    67681 ggttaatcgc tcctgaacca gcggaaaggc ggtaacattg tcgcgaaacc agcgcacgaa
    67741 atcggcctgc aacgtctgta aacgtgcttc gctgtgacca aaagtgcacc cccacagaag
    67801 cagactcatc ggccagcgtg ccgcacgacc ttttatcaat aacgcaatgg ctataatcgg
    67861 taacaacggc aggacaagta acgcattcag cggttggcga cgcagtaaat agcgcagaaa
    67921 actgccgaac atatcctgct gatgcaatgt tccatctaag tcaaaaaaca ccacacgacg
    67981 ctcgtgagtt gccaaaccat actcctctgg actgaaacat cctgattaat tttcgctact
    68041 acatagccta acagatagat catcactttt ccggcaacaa tcgaccgcat catggctaac
    68101 tggaattttt aattcatggc aattagcggc aatggaatat aaaattcact cgcgtgtctc
    68161 tcatattgcc tgttgtcgcc actatttcat cgcgaggaaa gatatgaacg gcttacttcg
    68221 tatccgtcag cgttaccagg ggcttgccca gagtgataaa aaactggcgg attatctgct
    68281 gctacaacct gatacggcgc gccatttaag ctctcagcaa ctggccaacg aagccggagt
    68341 cagtcagtcc agcgtcgtga agttcgcgca aaaactcggc tataaaggtt ttccggcgct
    68401 taagttggcg ttgagtgaag cgctggcaag ccagccggaa tcgccctccg tgcccattca
    68461 taaccaaatc cgcggtgatg atccgttacg gctggtcggc gaaaaactga ttaaagaaaa
    68521 tactgccgct atgtacgcaa cgctaaacgt taatagtgaa gagaaactgc atgaatgcgt
    68581 aacaatgttg cgctctgcgc ggcggataat tctgaccggt attggcgctt cgggtctggt
    68641 ggcgcaaaac tttgcctgga agctgatgaa gattggcttc aatgctgccg cagtgcgcga
    68701 tatgcatgcg ctactcgcaa cagtacaggc gtcgtcccct gacgatctgt tattagccat
    68761 ttcctacacc ggtgtacgac gcgagttaaa cctggcggca gatgagatgc tgcgagtggg
    68821 cggaaaagtg ctggcgatta ccggctttac tccgaatgcc ctgcaacagc gtgcttctca
    68881 ttgcctgtat accattgccg aagaacaagc gacaaacagc gcttcaatct ctgcttgtca
    68941 cgctcaggga atgttaacgg atttgctgtt cattgcgctg attcagcagg atctggaact
    69001 ggcaccagaa cgtattcgtc atagtgaagc gctggtgaaa aaactggtct gagtaaagaa
    69061 tgcgcgtata atgcccgccc ggtttgtgtt gttttgagag tttccttatg gcgttgttaa
    69121 tcactaaaaa atgcatcaat tgtgatatgt gtgaacccga atgcccgaat gaggcgattt
    69181 caatgggaga tcatatctac gagattaaca gcgataagtg taccgaatgc gtagggcact
    69241 acgagacacc aacctgccag aaggtgtgcc cgatccccaa tactattgtg aaagatccgg
    69301 cgcatgtcga gacagaagaa cagttgtggg ataaatttgt gctgatgcac cacgcggata
    69361 aaatttaata ttctactctg gaagtagagt at
//
LOCUS       DBANUU010000004        68190 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000004 DBANUU010000000
VERSION     DBANUU010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 68190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.004"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(228..863)
                     /gene="nth"
                     /locus_tag="ACIE68_000221"
     CDS             complement(228..863)
                     /gene="nth"
                     /locus_tag="ACIE68_000221"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFX5906598.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(863..1558)
                     /gene="rsxE"
                     /locus_tag="ACIE68_000222"
     CDS             complement(863..1558)
                     /gene="rsxE"
                     /locus_tag="ACIE68_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFX5906599.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(1562..2182)
                     /gene="rsxG"
                     /locus_tag="ACIE68_000223"
     CDS             complement(1562..2182)
                     /gene="rsxG"
                     /locus_tag="ACIE68_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFX5906600.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(2186..3244)
                     /gene="rsxD"
                     /locus_tag="ACIE68_000224"
     CDS             complement(2186..3244)
                     /gene="rsxD"
                     /locus_tag="ACIE68_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFX5906601.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(3245..5179)
                     /gene="rsxC"
                     /locus_tag="ACIE68_000225"
     CDS             complement(3245..5179)
                     /gene="rsxC"
                     /locus_tag="ACIE68_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFX5906602.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRATEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVA
                     AAIARAQAKKAAQQKVVNED"
     gene            complement(5172..5750)
                     /gene="rsxB"
                     /locus_tag="ACIE68_000226"
     CDS             complement(5172..5750)
                     /gene="rsxB"
                     /locus_tag="ACIE68_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFX5906603.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(5750..6331)
                     /gene="rsxA"
                     /locus_tag="ACIE68_000227"
     CDS             complement(5750..6331)
                     /gene="rsxA"
                     /locus_tag="ACIE68_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFX5906604.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(6408..6848)
                     /gene="ydgK"
                     /locus_tag="ACIE68_000228"
     CDS             complement(6408..6848)
                     /gene="ydgK"
                     /locus_tag="ACIE68_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFX5906605.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(6934..7149)
                     /gene="ydgT"
                     /locus_tag="ACIE68_000229"
     CDS             complement(6934..7149)
                     /gene="ydgT"
                     /locus_tag="ACIE68_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFX5906606.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(7422..7547)
                     /gene="blr"
                     /locus_tag="ACIE68_000230"
     CDS             complement(7422..7547)
                     /gene="blr"
                     /locus_tag="ACIE68_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFX5906607.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7790..8830
                     /gene="ydgJ"
                     /locus_tag="ACIE68_000231"
     CDS             7790..8830
                     /gene="ydgJ"
                     /locus_tag="ACIE68_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFX5906608.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(8865..9866)
                     /gene="add"
                     /locus_tag="ACIE68_000232"
     CDS             complement(8865..9866)
                     /gene="add"
                     /locus_tag="ACIE68_000232"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFX5906609.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLAKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(9970..11142)
                     /gene="malY"
                     /locus_tag="ACIE68_000233"
     CDS             complement(9970..11142)
                     /gene="malY"
                     /locus_tag="ACIE68_000233"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFX5906610.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(11152..12744)
                     /gene="malX"
                     /locus_tag="ACIE68_000234"
     CDS             complement(11152..12744)
                     /gene="malX"
                     /locus_tag="ACIE68_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFX5906611.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLNC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            12919..13947
                     /gene="malI"
                     /locus_tag="ACIE68_000235"
     CDS             12919..13947
                     /gene="malI"
                     /locus_tag="ACIE68_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFX5906612.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            14059..14826
                     /gene="hdhA"
                     /locus_tag="ACIE68_000236"
     CDS             14059..14826
                     /gene="hdhA"
                     /locus_tag="ACIE68_000236"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFX5906613.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            15055..15645
                     /gene="uidR"
                     /locus_tag="ACIE68_000237"
     CDS             15055..15645
                     /gene="uidR"
                     /locus_tag="ACIE68_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFX5906614.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            16034..17845
                     /gene="uidA"
                     /locus_tag="ACIE68_000238"
     CDS             16034..17845
                     /gene="uidA"
                     /locus_tag="ACIE68_000238"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFX5906615.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAANKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            17842..19215
                     /gene="uidB"
                     /locus_tag="ACIE68_000239"
     CDS             17842..19215
                     /gene="uidB"
                     /locus_tag="ACIE68_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFX5906616.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKMQQQ
                     LISDITN"
     gene            19254..20519
                     /gene="uidC"
                     /locus_tag="ACIE68_000240"
     CDS             19254..20519
                     /gene="uidC"
                     /locus_tag="ACIE68_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFX5906617.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSALGDRLNYWDVKMSIQYDFMLK"
     gene            complement(20564..22072)
                     /gene="ydgA"
                     /locus_tag="ACIE68_000241"
     CDS             complement(20564..22072)
                     /gene="ydgA"
                     /locus_tag="ACIE68_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFX5906618.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(22173..23348)
                     /gene="manA"
                     /locus_tag="ACIE68_000242"
     CDS             complement(22173..23348)
                     /gene="manA"
                     /locus_tag="ACIE68_000242"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFX5906619.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFSIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCQGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            23547..25193
                     /gene="fumB"
                     /locus_tag="ACIE68_000243"
     CDS             23547..25193
                     /gene="fumB"
                     /locus_tag="ACIE68_000243"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFX5906620.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            25336..26739
                     /gene="fumC"
                     /locus_tag="ACIE68_000244"
     CDS             25336..26739
                     /gene="fumC"
                     /locus_tag="ACIE68_000244"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFX5906621.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(26736..27665)
                     /gene="tus"
                     /locus_tag="ACIE68_000245"
     CDS             complement(26736..27665)
                     /gene="tus"
                     /locus_tag="ACIE68_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFX5906622.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(27741..29042)
                     /gene="rstB"
                     /locus_tag="ACIE68_000246"
     CDS             complement(27741..29042)
                     /gene="rstB"
                     /locus_tag="ACIE68_000246"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFX5906623.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(29046..29765)
                     /gene="rstA"
                     /locus_tag="ACIE68_000247"
     CDS             complement(29046..29765)
                     /gene="rstA"
                     /locus_tag="ACIE68_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFX5906624.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            29894..30229
                     /gene="ydgC"
                     /locus_tag="ACIE68_000248"
     CDS             29894..30229
                     /gene="ydgC"
                     /locus_tag="ACIE68_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFX5906625.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(30226..30948)
                     /gene="folM"
                     /locus_tag="ACIE68_000249"
     CDS             complement(30226..30948)
                     /gene="folM"
                     /locus_tag="ACIE68_000249"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFX5906626.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(30985..32367)
                     /gene="ydgI"
                     /locus_tag="ACIE68_000250"
     CDS             complement(30985..32367)
                     /gene="ydgI"
                     /locus_tag="ACIE68_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFX5906627.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATPPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(32553..33497)
                     /gene="ydgH"
                     /locus_tag="ACIE68_000251"
     CDS             complement(32553..33497)
                     /gene="ydgH"
                     /locus_tag="ACIE68_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFX5906628.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNAAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            34021..35553
                     /gene="pntA"
                     /locus_tag="ACIE68_000252"
     CDS             34021..35553
                     /gene="pntA"
                     /locus_tag="ACIE68_000252"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFX5906629.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            35564..36952
                     /gene="pntB"
                     /locus_tag="ACIE68_000253"
     CDS             35564..36952
                     /gene="pntB"
                     /locus_tag="ACIE68_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFX5906630.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(36977..38011)
                     /gene="tqsA"
                     /locus_tag="ACIE68_000254"
     CDS             complement(36977..38011)
                     /gene="tqsA"
                     /locus_tag="ACIE68_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFX5906631.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            38423..38788
                     /gene="mdtJ"
                     /locus_tag="ACIE68_000255"
     CDS             38423..38788
                     /gene="mdtJ"
                     /locus_tag="ACIE68_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFX5906632.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIVGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            38775..39104
                     /gene="mdtI"
                     /locus_tag="ACIE68_000256"
     CDS             38775..39104
                     /gene="mdtI"
                     /locus_tag="ACIE68_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFX5906633.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(39143..39964)
                     /gene="ydgD"
                     /locus_tag="ACIE68_000257"
     CDS             complement(39143..39964)
                     /gene="ydgD"
                     /locus_tag="ACIE68_000257"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFX5906634.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(40064..40147)
                     /locus_tag="ACIE68_000258"
     CDS             complement(40064..40147)
                     /locus_tag="ACIE68_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5906635.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(40240..40548)
                     /gene="asr"
                     /locus_tag="ACIE68_000259"
     CDS             complement(40240..40548)
                     /gene="asr"
                     /locus_tag="ACIE68_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFX5906636.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            40819..40923
                     /locus_tag="ACIE68_000260"
     CDS             40819..40923
                     /locus_tag="ACIE68_000260"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFX5906637.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(40972..42225)
                     /gene="ynfM"
                     /locus_tag="ACIE68_000261"
     CDS             complement(40972..42225)
                     /gene="ynfM"
                     /locus_tag="ACIE68_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5906638.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            42332..43225
                     /gene="ynfL"
                     /locus_tag="ACIE68_000262"
     CDS             42332..43225
                     /gene="ynfL"
                     /locus_tag="ACIE68_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFX5906639.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            43360..44580
                     /gene="mlc"
                     /locus_tag="ACIE68_000263"
                     /gene_synonym="dgsA"
     CDS             43360..44580
                     /gene="mlc"
                     /locus_tag="ACIE68_000263"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFX5906640.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            44705..45400
                     /gene="bioD"
                     /locus_tag="ACIE68_000264"
     CDS             44705..45400
                     /gene="bioD"
                     /locus_tag="ACIE68_000264"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFX5906641.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(45353..46645)
                     /gene="clcB"
                     /locus_tag="ACIE68_000265"
     CDS             complement(45353..46645)
                     /gene="clcB"
                     /locus_tag="ACIE68_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFX5906642.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFHHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVGLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(46804..47418)
                     /gene="dmsD"
                     /locus_tag="ACIE68_000266"
     CDS             complement(46804..47418)
                     /gene="dmsD"
                     /locus_tag="ACIE68_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFX5906643.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(47461..48315)
                     /gene="ynfH"
                     /locus_tag="ACIE68_000267"
     CDS             complement(47461..48315)
                     /gene="ynfH"
                     /locus_tag="ACIE68_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFX5906644.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLACIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWVCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(48317..48934)
                     /gene="ynfG"
                     /locus_tag="ACIE68_000268"
     CDS             complement(48317..48934)
                     /gene="ynfG"
                     /locus_tag="ACIE68_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFX5906645.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(48945..51368)
                     /gene="ynfF"
                     /locus_tag="ACIE68_000269"
     CDS             complement(48945..51368)
                     /gene="ynfF"
                     /locus_tag="ACIE68_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFX5906646.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVVQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGKEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREAPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(51429..53855)
                     /gene="ynfE"
                     /locus_tag="ACIE68_000270"
     CDS             complement(51429..53855)
                     /gene="ynfE"
                     /locus_tag="ACIE68_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFX5906647.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAATV
                     QQASEKVIWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGTRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            complement(54054..54359)
                     /gene="ynfD"
                     /locus_tag="ACIE68_000271"
     CDS             complement(54054..54359)
                     /gene="ynfD"
                     /locus_tag="ACIE68_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFX5906648.1"
                     /translation="MKLSTCCAALLLALASPVVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            54467..55177
                     /gene="ynfC"
                     /locus_tag="ACIE68_000272"
     CDS             54467..55177
                     /gene="ynfC"
                     /locus_tag="ACIE68_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFX5906649.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(55180..55740)
                     /gene="speG"
                     /locus_tag="ACIE68_000273"
     CDS             complement(55180..55740)
                     /gene="speG"
                     /locus_tag="ACIE68_000273"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFX5906650.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(55775..56116)
                     /gene="ynfB"
                     /locus_tag="ACIE68_000274"
     CDS             complement(55775..56116)
                     /gene="ynfB"
                     /locus_tag="ACIE68_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFX5906651.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            56251..56577
                     /gene="ynfA"
                     /locus_tag="ACIE68_000275"
     CDS             56251..56577
                     /gene="ynfA"
                     /locus_tag="ACIE68_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFX5906652.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            56783..57997
                     /gene="rspA"
                     /locus_tag="ACIE68_000276"
     CDS             56783..57997
                     /gene="rspA"
                     /locus_tag="ACIE68_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFX5906653.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            58009..59028
                     /gene="rspB"
                     /locus_tag="ACIE68_000277"
     CDS             58009..59028
                     /gene="rspB"
                     /locus_tag="ACIE68_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFX5906654.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(59216..60496)
                     /locus_tag="ACIE68_000278"
     CDS             complement(59216..60496)
                     /locus_tag="ACIE68_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFX5906655.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKDVRVRENLGVPDTAKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
     gene            complement(60531..60782)
                     /locus_tag="ACIE68_000279"
     CDS             complement(60531..60782)
                     /locus_tag="ACIE68_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFX5906656.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            complement(60855..63326)
                     /locus_tag="ACIE68_000280"
     CDS             complement(60855..63326)
                     /locus_tag="ACIE68_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFX5906657.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPSDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGLQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHNSDNETGETADHP
                     IKVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPSQADS"
     gene            complement(63419..63610)
                     /gene="ydfD"
                     /locus_tag="ACIE68_000281"
     CDS             complement(63419..63610)
                     /gene="ydfD"
                     /locus_tag="ACIE68_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HFX5906658.1"
                     /translation="MNSAFVLVLTVFLVSGEPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            complement(63607..63795)
                     /gene="dicB"
                     /locus_tag="ACIE68_000282"
                     /gene_synonym="ftsT"
     CDS             complement(63607..63795)
                     /gene="dicB"
                     /locus_tag="ACIE68_000282"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFX5906659.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            complement(64363..64581)
                     /gene="ydfC"
                     /locus_tag="ACIE68_000283"
     CDS             complement(64363..64581)
                     /gene="ydfC"
                     /locus_tag="ACIE68_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFX5906660.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(64611..64739)
                     /gene="ydfB"
                     /locus_tag="ACIE68_000284"
     CDS             complement(64611..64739)
                     /gene="ydfB"
                     /locus_tag="ACIE68_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFX5906661.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSNG"
     gene            complement(64741..64896)
                     /gene="ydfA"
                     /locus_tag="ACIE68_000285"
     CDS             complement(64741..64896)
                     /gene="ydfA"
                     /locus_tag="ACIE68_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFX5906662.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(65063..65470)
                     /gene="dicA"
                     /locus_tag="ACIE68_000286"
     CDS             complement(65063..65470)
                     /gene="dicA"
                     /locus_tag="ACIE68_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional dual regulator DicA"
                     /protein_id="HFX5906663.1"
                     /translation="METKNLTIGERIRYRRKNLKHTQRSLAKALKISHVSVSQWERGD
                     SEPTGKNLFALSKVLQCSPTWILFGDEDKQPTPPVEKPVALSPKELELLELFNALPES
                     EQDTQLAEMRARVKNFNKLFEELLKARQRTNKR"
     gene            65554..65784
                     /gene="dicC"
                     /locus_tag="ACIE68_000287"
     CDS             65554..65784
                     /gene="dicC"
                     /locus_tag="ACIE68_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicB transcriptional regulator DicC"
                     /protein_id="HFX5906664.1"
                     /translation="MLKTDALLYFGSKTKLAQAAGIRLASLYSWKGDLVPEGRAMRLQ
                     EASGGELQYDPKVYDEYRKTKRAGRLNNENHS"
     gene            65768..66289
                     /locus_tag="ACIE68_000288"
     CDS             65768..66289
                     /locus_tag="ACIE68_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFX5906665.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTYEGGAIDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REITDGKSITCMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            66270..67235
                     /locus_tag="ACIE68_000289"
     CDS             66270..67235
                     /locus_tag="ACIE68_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5906666.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLSESIGSKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDPQTTEQDFLTRNSDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVGLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            67276..67698
                     /locus_tag="ACIE68_000290"
     CDS             67276..67698
                     /locus_tag="ACIE68_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFX5906667.1"
                     /translation="MAKVFTQEEREKIKWQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSSPDG
                     EIRRYDSRLNIICRECRKSEVMQRILAFYQGNFQDVAQ"
     gene            67695..68184
                     /locus_tag="ACIE68_000291"
                     /pseudo
     CDS             67695..68184
                     /locus_tag="ACIE68_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145667.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
BASE COUNT        17121 a        16577 c        17352 g        17140 t
ORIGIN      
        1 ttaacctgat tcttgcaatg tttttacatt gcgacagcat cgtgatcttg atcacgaatg
       61 aatagaaaat ttcgctatta aaaaaacaat taacctgcat ttttggttat ccgcaaacgg
      121 attacctgat gataacgcta agcaatggca tcattgacgt taaaaagatg ttttaaaaga
      181 ggataaagaa aggttatcaa tggggtaatc ggtgttaccc cttttcttca gatgtcaact
      241 ttctctttgt attcacaaag atcttcaata atacaagagc cacagcgggg cttgcgggca
      301 atgcaggtat aacgcccgtg caggatcaac caatggtggc agtcgacttt aaactctgcc
      361 ggaaccactt tcagtagctt ttcttctacc tgttcgacgt ttttccccgg cgcaaattga
      421 gtacgattac aaacgcggaa aatgtgcgtg tcgacagcaa tagtcggcca gccgaatgca
      481 gtgtttaata cgacgttggc tgttttacga cctacgccgg gcagggcttc aagcgcagca
      541 cgatcttccg gaacctcgcc attatgctgc tccagcaaga tacggcaggt tttgatgata
      601 ttttctgctt tgctgttata aagcccaatc gttttgatat aggttttcac cccttcaacg
      661 cccagttcaa gcatcgctgc aggcgtattc gccaccgggt agagtttcgc cgtcgcctta
      721 ttaacactga catcggtcgc ctgagcggaa agcagtacgg caatcagcaa ttcaaaaggc
      781 gaactgaaat taagctcggt ggtgggatga ggattgttct cacgcaggcg agtgaggatc
      841 tccaggcgtt ttgctttgtt catcagacat tccctgtttc accgtttggc aatgcacgtt
      901 cggcagctgc ttcagcacgg cgctttttca ttctttcatc aatcaggtat tttcctgcca
      961 gcatcagtcc caggccaata aatgcacctg gaggcagcat cgccagcagg aaaggggagt
     1021 cggtgtggaa aatttccacg cgtaatactt ttgcccagct acctaacagc gcatctgcac
     1081 cgtcaaacaa tgtgccattg ccgataattt cgcgtagtga acccagcacg aacatggcgc
     1141 aggttgcgcc cataccaatt gaaaagccgt ccagtgccga aagcgccgga ccttttttgg
     1201 cggcgaaggc ttcagcgcgg cccacaacga tacagttagt gacaatcagc ggaataaaaa
     1261 tccctaatga ttgatatagg ccaaaggcgt aggcgttgat cagcatctgt acagcgctga
     1321 ccaccgaggc gatgatcatc acgtaaatgg gaatgcggat ctcggctggc gtccagtgac
     1381 gcagcgtcga aatggtcagg ttggtcagcg tcagtaccag cgtagtcgca agtcctaaac
     1441 ccagagcgtt agtggcagtg gacgtgaccg ccaacagagg acaaaggccg agcaactgga
     1501 ccagagcaga gttgtttttc cacaacccct gaacaataac gtctttaatt tcgctcacgc
     1561 tttattctcc acaggcagga agttgagaaa gttgtgccgg taacgtctga gcgtacaatc
     1621 cggcgcgttt taccgcatta accaccgcgc ggggagtaat cgtcgcgccg gtgaactggt
     1681 cgaaatcacc accatctttc ttcaccgccc agtgcgcatc atccgcacca ctgatttttt
     1741 tacccgcaaa atgggtgatc cagtcagaaa ggcgcagttc gattttatcg ccaagccctg
     1801 gcgtttcgtg gtgctctgtc acgcgcgtgc caagtaccgt gccgttaaaa tcggctccca
     1861 ccagcagctg aatcgcaccg gaatagccat ctggcgcggt tgcttccaga acggcggcta
     1921 ccggtttgtc atcctgtttg gcgatgtaaa cccgatgctc acctttacct aactctggcg
     1981 cagttaccag atagcaactc tgtgccagcg cattgttata gcgttcggct ggcagcacct
     2041 gatcaaataa tgccttttgt tgcagactgg cctgttcagc aatcgtcgtt ttggtcatct
     2101 ggttgatggc cgcagttaac cctgttgaac ccgctgcaaa tagcgctaac gtaatgccgt
     2161 gttttcggat agttttcagc atggtttacc ctttgcgatg gccgtagacg cgcggacgcg
     2221 tgtagtaatc gatcagagga accgtgatgt tcgccagcag gacggcaaaa gccacgccgt
     2281 caggatagcc gccgaaactg cggatcaacc agactaataa gcccgccagc gcgccgaaaa
     2341 taagacgacc acgattggtc gtagaagcgg taaccgggtc agtcaaaata aagaatgcgc
     2401 cgagcatggt cgcaccagac agcagatgaa tttgcggtgc tgccagtgtt tctggtgaga
     2461 acaaccagcc caacgttgcg cataacgcca gcgttactaa gaagctgagg ggaatatgcc
     2521 agcgaatcgc tttctgccat agcaaccaca ggccgccagc cagccaggcg agatttaccc
     2581 attgccaacc agcgcccgcc agaataccgc tgtagatcgg atattgcata atctgttcaa
     2641 ccgaatgacc ggcacggaca gaggttttaa atgtatccag cggtgtcgcc tgactaatgc
     2701 catcaatacc taagcgtagt gtgttcatat caccaccact ggcggtatgt ccgctgaaaa
     2761 taacctgtat ggcgtcgata aaaccaggga tgttgaccgc aatttcatgt ggcggtaacc
     2821 agctggtcat ctgcaccggg aaggagatca gtaagaccac ataaccaatc attgccggat
     2881 taaacgggtt ttgccccaga ccgccataca actgtttagc gatgatcacc gcaaacaccg
     2941 tacccagcac gaccatccac catggcgcga gggggggaat acttaccgcc agcaataagc
     3001 ctgtcagcaa tgctgagtta tctttcaacg ttgcggctac cgactgcttg cgtagtttga
     3061 gtacgagagc ttcggctaac agagcactaa ccgatgccaa caggatctga acgagagtac
     3121 cccaaccaaa aaaccacagt tgcgctgcga ttcctggcac ggctgcgagc aacaccaaca
     3181 gcataatgcg cgatgtctgg cgctggttat gggtataagg ggagctagct attctgaata
     3241 ccatttagtc ctcgtttaca accttctgct gggcggcttt tttggcctga gcgcgggcaa
     3301 tagccgcggc aactgccgct ttgcgcggat caacctgttc ttctggttct gcgttagtct
     3361 gttgctgttc cagcttgcgc gctttggcac gggcaatagc ggcttcgacg gcggctttgc
     3421 gcggatcgac ctgttcttct ggttccgcat tagcctgttg ctgttccagc ttgcgcgctt
     3481 tggcgcgggc gatagctgct tcaacggcag ttttacgtgg atcagcaacg gttgctgcgt
     3541 cgttagtttg ctgcagttct gcctgtttcg ctctggcttg cgctttacgg gcttcccgtg
     3601 ctgcaataat tgcactgtta tccgggcgtt cgcccgcttt aatcacaata ggctgtgtag
     3661 cctgggcctg tttctctttc acccgcgcca gagcggcagc aatcgcatct ttatctttgg
     3721 ctgcaggttg aacggctgcg ctcttatgtc gttcaaggcg agccgctttt tcgcgctcca
     3781 gacgagcctg gcgcgcttcg aaacgcgctt tggcttctgt ggcgcgcttt tcttcctgac
     3841 gaatagccgc aatttcagct ttttcctgac ggaaatattg caccagggga atattgctcg
     3901 ggcaaaccca cgcgcaagcc ccacattcaa tgcaatcagc aatgttatgc gtggtagctt
     3961 tatcgtgttg ctgacctttg ctgaaccagt acaactgttg cggcaaaaga tccgcagggc
     4021 aggcgtcagc acaggcgcta caccggatgc agctttgttc ttcctgtggt tcgccaagtt
     4081 cattggcaga gggagccaac agacagttgg taatctttac gaccgggaca tccagccatg
     4141 gcaaggtaaa gcccattagc gggccaccca taatcaccat ttgatcggca gaggggcaga
     4201 atccggcatc attcaataaa tgacgcactg gcgtccccag ccgtgcccag acgttgcccg
     4261 ggcgagcgat tgcttcgcca gtcagggtta caacacgctc ggtaatcggc tcgccatcaa
     4321 taacggcacg tttcactgca taagcagtgc cgacgttttg cattaatacg ccgatatcgg
     4381 atgaacgccc gccatgtgga acctgcttcc cggtcagaat gtaggttaat tgtttagcac
     4441 cgccagaagg atatttggtt ggaatcaccc gcagagaaat atcgttagag tccgccagca
     4501 ccgcgcgcag catggaaatc gcctgcggtt tgttatcttc aatgccgata agaatttcgc
     4561 gtggctgcag aatatgcgca agaatgcgaa taccctctac gacctgagcc gcgcaatcct
     4621 gcatcaaacg gtcatcggcg gtaatgtacg gctcgcactc agccgcgttg ataatcaacg
     4681 tttcaatctt atctccgcca ccctgcaatt taacgcctgt cgggaatcct gcaccgccca
     4741 gcccggctac accaaactga tgtatgcgct cgattaactc ttcgcgactg cgagtgcgat
     4801 aatcggccca gccgtcgcgc gggatccagc agtcttcacc atcggcatca ataatcacgc
     4861 ttaattcagc taaagctgaa ggatgagccg tagagtgggg cgcaatagcc gtaacggtac
     4921 ccgaggtggg cgcgtgaaca ggcagcattt tgccgcgacc acgggtaagc ggctggccgc
     4981 gcaatacttt atcgccgacg ctaacgcaca actcaccttc agcgccaata tgctgtttca
     5041 gtggaataac aaaacgctgc gccagtggta cctggcgcag cggtgtaccg ttggactggg
     5101 ttttcatctc cggtggatgg atgccgccgt tgaaatccca gattttattt tttctgaatg
     5161 cagagaataa cttaagcatg gtgttccacg ggaatgatac gcacgggaat ggtgttcaga
     5221 tcccatttcc aggagtcagg tgtttctgcg accggttgca acgagatgca gtgcgtcggg
     5281 cacggatcaa cacataaatt gcagcccgta cagagatcac tcattaccgt atgcatggca
     5341 cgggtagcgc caacgatggc gtctaccgga cacgcctgaa tacatttagt gcagccaata
     5401 cagttatttt catcaataac cgccaccatc cgcgcaggcg ttagctcttg cgcttcgcca
     5461 tccagcggct gcggctcgac attaagcaac tcggcaattt ttagcatcac agcttcgcca
     5521 cctggggcgc aacggttgat tttttcaccg ttacagctga tggcttccgc gtaggggcga
     5581 cagccgggat aaccgcactg accacactgg ctctgcggta agatttcgtc aattttctca
     5641 acgaccggat cgtcttccac cgcaaaacgg cgggaggcat aacccagaat ggcgccaaac
     5701 gccaggccca gcaggctcac ggcggcaacg gcaatccaga tagcattcat tacaacttca
     5761 ccaaaccact aaagcccata aaggccagag acataagacc tgcggtaatt aacgcaatgg
     5821 cattaccgcg aaaaggtgcc gggacatcag ccacagcaag gcgttcgcgg atggctgcga
     5881 agagaaccat caccagcgaa aaaccgacag cggcggaaaa accgtacagc gccgactgca
     5941 agaaattgtg cccgagattg atattcagca acgccacgcc gagcacggca cagttggtgg
     6001 tgataagcgg caaaaaaatc cccagcaaac ggtaaagcac cgggctggtt ttgcgcacca
     6061 ccatctcggt gaactgcacg accacagcaa tcaccagaat aaatgccagg gtgcgcaagt
     6121 aaatcagatt aagtgggatc aaaatccacg tatcgataag ccaggcgcaa atagacgcca
     6181 gcgtcatcac aaacgttgtt gccagcccca tgcccatcgc ggtttccagt tttttggaaa
     6241 cccccataaa cggacagaga ccgagaaact tgaccagtac aaagttattg accagtacag
     6301 ttccgacaaa cagtaacagg tagtcagtca tttttcggcc tgaaataaaa aagccgccta
     6361 ttatccgtta atccagagca ggcgacaaca ggttaactgt aaggttatta cggattcaca
     6421 aacgtcgctt ttacccgcga cgagcgtttg aaatagggga cgatcaatgc ggttgccaac
     6481 aaagtaaaca gcaactggcg aacggctatg ccatcttcca cgggtgaaaa agcgaatgct
     6541 tttactgcca gcaatacgga aataagcagc cagataatat agtgtttagg aacacaacgc
     6601 cgacgtttaa agaaagcgat ggtcaaccac agtgtgtaat accacatggc gatagcggta
     6661 ataaaagaaa cgccccataa gatttgtgtc gtcagtgctt gtccgccgag cgtttgaaat
     6721 gtttgcggag aagataacgc agcggtatac agcaacaacg ccagcgtcgt actcaacaga
     6781 gcgactaaaa gccaggccaa cgggccaagc aaccagcctc caatacgttg tggcgttgtg
     6841 gtggtcatag gttctccaga atagcgacgc aaacaggcaa atctaatttt aggggcgtta
     6901 ttataaggct tttataaccg atcgctactt tctttattgg acatagtgcc agacggactt
     6961 cggtacctgg ccgaggtcaa acaaacgccc gccagaaacc agctctgcgc gacggtgatc
     7021 ggcagcacga tacatattga tcagttcctg atcgtccgtc agggtgtaat taagatggtc
     7081 gtagagtttt tccagacttt cgagtgaact gattttgcga aattttaata agtagtcctg
     7141 aacagtcata aaaggtccat aaaataatta ataccaaagg ggtaggtatt attagtgatg
     7201 ataaaatttc accaaaagtg aacgcttgtg ttattacaat aataagaatg cgctgatggc
     7261 aaatattcag ggatgaaaaa ctgctctgaa aagagtaacg cgctttacta tccctgacaa
     7321 tcaccaacaa catcgaacaa gataataaat tcctggttta atatccgaca agtgaaaaca
     7381 tgcacccgga cgggcagcat gtcgctccac aagtgcagag cttacttgtg ttgtaccgaa
     7441 gcactctgtt caggtggctg atagttgtca atgtgactcg ccacgccaag aagaatgact
     7501 gaaacgacaa gaacgatcca acctgttaat tcaataagac gattcattac agcccacatc
     7561 tctcttgatt gatccattaa cttcaggggg taaatgttac ttagcaataa tagctccagc
     7621 aagattttta ctgaggtttt tgcgatattt agcttttgtc gttggaaaat tcgctatttt
     7681 tttgacttaa gttaaacaac atcccttatt gctggcaggt tattaaactg ttgagcgtgg
     7741 gtaaaggata gtgtcaaata gccatcatac ttcaatgaga ggcaatgaca tgagcgacaa
     7801 catccgtgtt gggttgattg ggtatggtta tgcgagcaaa accttccatg cgcccctgat
     7861 tgcgggcacg cccgggctgg aactggcggt aatctccagc agcgatgaaa caaaagtaaa
     7921 agccgactgg ccaacggtta cggttgtctc tgagccgaag catctgttta acgatcccaa
     7981 catagacctg attgtcattc ctacacccaa cgatacccat ttcccgttag ccaaagcggc
     8041 gcttgaggcg ggtaaacatg tggtcgttga taaacccttt accgtgacac tgtcacaagc
     8101 gcgagagctg gatgcgctgg caaaaagcct ggggcgtgtg ctgtctgtat tccataaccg
     8161 tcgctgggat agcgatttct tgacgctaaa aggtttactc gcggaaggcg tgctgggtga
     8221 agttgcttac tttgagtctc attttgaccg cttccgtccg caggtgcgcg atcgttggcg
     8281 tgaacagggc ggcccaggca gcggtatctg gtacgattta gcaccacatc ttcttgatca
     8341 ggccattacg ctttttggtt taccggtcag catgacggta gatttggcac agttacggcc
     8401 cggagcgcag tcgaccgatt atttccacgc catcttgtcc tatccacagc ggcgagtcat
     8461 tttacacggt accatgctgg cagccgctga gtcagcacgg tatatcgtgc atggatcccg
     8521 aggcagttat gtgaaatatg gcctcgatcc acaggaagaa cgtctgaaaa atggcgagcg
     8581 tctaccgcag gaagactggg gctacgatat gcgtgatggc gtacttaccc gcgtggaagg
     8641 tgaggaacgt gtcgaagaaa cgctgttgac ggtgcctggg aattatccgg cttactatgc
     8701 ggctattcgt gatgcgttaa atggcgatgg tgaaaatccg gttccggcaa gccaggcaat
     8761 ccaggtaatg gagttgattg agctgggcat cgaatccgcc aaacatcgcg cgactttgtg
     8821 ccttgcatga aaaatggatg gtgcaatttg caccatccat tttgttactt cgcggcgact
     8881 ttttctcgca gtgcgcgttt ttcctcagcg ctgaggaaag ccatttccag accattaatc
     8941 tgtgcctggc ggatttgctc gcgggataac ccagcagctg gcgcggcaac ggtatattcg
     9001 tgaatgatat ccactccctg tacgccggga tcgtcagtgt taatgctggc acgaatgcca
     9061 tgctcaagga acgttttcag cggatgtgca gccagctctg ctacggtgct ggtctgaata
     9121 ttggaggtca gacaggattc aataccaatt tgttgctcgg cgagaaaatc catcagcgcc
     9181 cgatcttcaa tggcttttac gccatgtcca atacgctccg cacccagttc acgaatcgcc
     9241 tgccagatgc tttccggccc ggcagcttcg cctgcatgga cggtaatatg ccagcccgca
     9301 tcacgcgcgc ggttgaagtg agaaaggaac agacttcccg ggaaaccaag ttcatcaccg
     9361 gctaaatcaa gtgcggtaat ctggtcacgg tgggctaaaa aggcctccag ctcttgctga
     9421 caggcggctt cgccgaaggt ccggctcata atgccgataa gcttcgcctg cacaccaaag
     9481 gtgcggcaac cttcacgtac gccatcgatc accgcttcga caacacccgc tacaggcagc
     9541 tgatgtgcca ttgccatgta gcctggtgaa aaacgcagct cgacatagtg caggccgtga
     9601 cgggctgcat cttcaatgtt ttcaaatgcc acgcggcgac aggcatcaag agaggcgaga
     9661 actttaacgc cccagtcaag tttggccaga aagctcacca gatcgggttc gttggcaatg
     9721 acctgaacgt ggggaatcag tgtttccagg gattgtgcag gaagcgagat attatactgg
     9781 cggccaagtt caagaatggt ctggggacga atgttgccat caaggtggcg atggatatca
     9841 gttaatggca gggtggtatc aatcatggtc gcactctttt tctggatata aaagtgcgca
     9901 ccattataaa aacaaagcag ggtaaaaatc cacgattgcg caacgatttt ttacgttgcg
     9961 caattggggt taacgaacag cgcggatggc gttaattaat ccagccacac ctttttccag
    10021 tttcgaacgt gggcagccgg cattgagacg gacaaaacca cgaccttctt caccgtaggt
    10081 ataccccggc atgatcgcga ctttttcttg ttcgataagt gctttttgca acgcgttgtc
    10141 gtcaatattc aacggacgta aatcaagcca tgccagataa gtggattgcg ggatctgcca
    10201 gttgagttca ggaaatgcgg cgttcatttt atctgcgata tacgtcaggt tatctttcag
    10261 atagatgcgt aaggcatcca gccacggcgc gccttgctga taggcggcga tatgggcagt
    10321 taacgccagt accgaagggg aagaaagccc atcacggcct ttcagtgccg ataaataggc
    10381 atcacggctg ctgctatttt ctataatccc ataagcaccg gtcagcgcgg gaatattgaa
    10441 acttttcgat cccgacgtca gcaacgccca gtctccccgc gccacattac tccagggaat
    10501 atgcggctgc tcgccccaga ccatatccat atggatttca tcggaaataa cccgcacacc
    10561 atgacgctcg cataggtcag ccatgatctc cagctcatcg cacgtccaca ctttaccggt
    10621 aggattctgt gggctacaca ggagcataat tttacattct ggtttcgcca acacggcttc
    10681 cagcttgccc atatcgcaaa accagccgtc agcctgcttc tctaaagcaa cgggcattac
    10741 tgtgcgctgg ttaccttcaa tggccttgta aaatgcgtca taggcgggcg tgtggatcac
    10801 cacgccttca cctgtttcag accactgacg aatcagttct gaaaccatat agatgacaga
    10861 agggccatac accaccgtct gcgtatcaat tgcggtgtaa tgctgggtgg aaaaccagtg
    10921 ggcaatagcc gcgagaaact catcgttttt ccagcggctg tagccaaata cgccgtgcat
    10981 caggcgctga ttcagcgcct cgataatgca gggggcagtg gcaaaatcca tgtctgaaat
    11041 cgtgaacggt aacaggtcag cagtgccgaa acggtcagcg acataatccc actgcgtgca
    11101 ccatgtgcca tgacgatcca cgacctttga aaaatcgaac atatcgtatc cttatgcctg
    11161 gacagtatgc atcagaccgg ccatttcatc ttttactgac tgaacttgtg gcccgataac
    11221 aacctgcagg ttatgttgat taagttgtac tacgccaatt gcccgattgt ccttcagtgc
    11281 ctgcacatta acaagcgaca tatctttcac agacaaacgc agacgggtaa tgcagttatc
    11341 gaggctgaca atattgtcgg caccgcctaa tgcttcgagg attgcaggaa cgttgtaacc
    11401 tgatttaccc ggcgcaccgg caacggcttt ttcgattgag ctggcaactt cgctatcgcg
    11461 ccccggggtt ttcagattga agcgggtgat agcgaaacgg aagatgacgt agtaaacgac
    11521 aaaccagatt gccgccacca ctggcaccat gtaccacttg gttgacagac catgcaaaat
    11581 accgaacacc acgaagtcga tgatattgcc gtcggtatta ccgatggtga cgccgagcac
    11641 agacatgacg gtgaagccga ggccggttaa cagcgcgtgg atgacataca gaactggcgc
    11701 tacgaacagg aacaggaatt ccagcggttc ggtagtgccg ccaacgacgc aggcgatcag
    11761 gccagaaatc agcagacctt taattttatg gcgattttcc gggcgcgcgc agtgatacat
    11821 agctaacgct gcacctggca gaccgccgag aaacgcaggc attttacctt gcgaaaggaa
    11881 acgcgtggcg ctttcagaaa aaccgtgagt ggtcgggcaa ttcaattgcg cctggaagat
    11941 ggtcagtgcg ccgctgacgg tttgaccgca gacttcctgc gtgccgcctg cgtcggtaaa
    12001 gcgaattaat gccaccagaa tgtgatgcag accaaacggc aacagcagac gttcaccggt
    12061 accaaacagc atcggtccga aatcacccgc gctgtttatc atatggccca agccgctaat
    12121 acccatggcg aaaatcggcc agactaatgg aatcaccagg ccgacaaggc ccatcaccag
    12181 cgaggagata attggtacga agcgcgtacc gccgaagaat gccagcgcat ccggcaggcg
    12241 gatattatgg aaacgctcat gcagcatcca gacgataata ccggcgatca ccgcaccgag
    12301 gatcccggta tcgatcgact ggatcccaag aatgctctgg atgttattgg ctttcagaac
    12361 cgcggcatcc gtggttggca gaatgccttt attggtcaac cagaagttta ccgcgaggtt
    12421 cattaccgca taaccgacga agccagcgaa tgccgctacg cctttgtttt cgcgtgccag
    12481 gcccagcggg atggcgatac agaacatgac aggcaggaaa ctaaaagcaa acgagccaat
    12541 cttactcatc caggtaaaga tagcctgcaa cacggggttg cccaggaccg ggattagggt
    12601 gatgacatcg tggctactaa gggaactgcc aatgccgagc ataatgccgc agaacgacaa
    12661 taatgccacg ggtaacatga aggttttgcc taactgctgg aagaactccc acagcgtgac
    12721 tttcggtgct gttttcgccg tcataaaacg actcctcgta gagaacaatc tggattcagt
    12781 aaattgcgcg agaagataaa acgttttatc aaattttagt gaggcataaa tcacattacg
    12841 caacgataat agcgggtata agataaataa aaggtaaaac gttttatctg tcacataatc
    12901 agggagtagg tcatctgcat ggctaccgcc aaaaaaataa ccattcatga tgttgcgctg
    12961 gctgcgggcg tgtcggtaag taccgtttcg ctggtgctta gtggcaaagg gcgaatctct
    13021 accgccacag gagaacgcgt taacgccgcc attgaagagc tgggatttgt gcgcaatcgc
    13081 caggcgtcgg cgctgcgcgg cggacaaagc ggcgtcattg gtttgatcgt ccgtgattta
    13141 tctgcgccgt tttacgccga attgacggcc ggattgacgg aagctctgga agcgcaggga
    13201 cggatggttt ttttgcttca cggcggtaaa gacggcgagc agctggcaca gcggttttca
    13261 ctgttactga atcagggtgt cgatggtgtg gtaatagccg gggctgcagg aagcagcgat
    13321 gacctgcgac ggatggcaga agaaaaagct atcccggtga ttttcgcttc ccgtgccagt
    13381 tatcttgatg atgttgatac ggttcgcccg gacaacatgc aggctgcaca gttgttgacg
    13441 gagcatctca ttcgcaatgg gcatcagcgg atcgcctggc tgggagggca aagttcctca
    13501 ttaacccgtg cagaacgggt ggggggctat tgtgcaactc tactaaaatt tggcctgccg
    13561 tttcacagcg attgggtgtt ggagtgcact tccagccaga agcaagcagc ggaagctatc
    13621 acggcgcttt tacgtcataa cccgaccatc agtgccgtgg tttgctataa cgaaactatt
    13681 gcgatggggg catggtttgg tttgctgaaa gcagggcggc aaagcgggga aagcggagtc
    13741 gatcgttact ttgagcaaca ggtttcgctg gcggcattta ccgatgcgac accaaccaca
    13801 cttgatgata tacctgttac ctgggccagc acgccagcgc gggaacttgg taccacactt
    13861 gcggatcgca tgatgcaaaa aatcacccat gaagagacgc attcacgcaa tcttattatt
    13921 cccgcccggc tcattgcggc gaaataatcc tctctttatc tgctatacct ggtagtgtcc
    13981 tttcctcaag gttaatgata aaaaatgtgg cacacaggcg gtgcctttta tttttactga
    14041 agttcacagg aggtttatgt gtttaattct gacaacctga gactcgacgg aaaatgcgcc
    14101 atcatcacag gtgcgggtgc aggtattggt aaagaaatcg ccattacatt cgcgacagct
    14161 ggcgcatctg tggtggtcag tgatattaac gccgacgcag ctaaccatgt tgtagacgaa
    14221 attcaacaac tgggtggtca ggcatttgcc tgccgttgtg atattacttc cgaacaggaa
    14281 ctctctgcac tggcagactt tgctatcagt aagctgggta aagttgatat tctggttaac
    14341 aacgccggtg gcggtggtcc taaaccgttt gatatgccaa tggcagattt tcgccgtgct
    14401 tatgaactga atgtgttttc ttttttccat ctgtcacaac ttgttgcgcc agaaatggaa
    14461 aaaaatggcg gtggcgttat tctgaccatc acttctatgg cggcagaaaa taaaaatata
    14521 aacatgacat cctatgcatc atctaaagct gcggccagtc atctggtcag aaatatggcg
    14581 tttgacctgg gtgaaaaaaa tattcgggta aatggcattg cgccgggggc aatattaacc
    14641 gatgccctga aatccgttat tacaccagaa attgaacaaa aaatgttaca gcacacgccg
    14701 atcagacgtc tgggccaacc gcaagatatt gctaacgcag ctctgttcct ttgctcgcct
    14761 gcagccagct gggtaagcgg acaaattctc acagtctccg gtggtggggt acaggagctc
    14821 aattaataca ctaacggacc ggtaaacagc cgtacgtgtt gtttaccggg atagcgtgtg
    14881 caattcatca acgtctctgc taaacaactg gcagccaaat cacggctatt ggttaaccaa
    14941 tagctggatg aaaagcatac gaatagagtg tgccttcgca ctattcaaca gcaatgatag
    15001 gcgctcacct gacaacgcgg taaactagtt attcacgcta actataatgg tttaatgatg
    15061 gataacatgc agactgaagc acaaccgaca cggacccgga tcctcaatgc tgccagagag
    15121 attttttcag aaaatggatt tcacagtgcc tcgatgaaag ccatctgtaa atcttgcgcc
    15181 attagtcccg ggacgctcta tcaccatttc atctccaaag aagccttgat tcaggcgatt
    15241 atcttacagg accaggagag ggcgctggcc cgtttccggg aaccgattga agggattcat
    15301 tttgttgact atatggtcga gtctattgtc tctctcaccc atgaagcctt tgggcaacgg
    15361 gcgctggtgg tcgaaattat ggcggaaggg atgcgtaacc cacaggtcgc cgccatgctt
    15421 aaaaataagc atatgacgat cactgaattt gttgctcagc ggatgcgtga tgcccagcaa
    15481 aaaggcgaga taagcccaga catcaacacg gcaatgactt cacgtttact gctggatctg
    15541 acctacggtg ttctggccga tatcgaagcg gaagatctgg cgcgtgaagc gtcgtttgct
    15601 cagggattac gcgcgatgat tggcggtatc ttaaccgcat cctgatcctc tcatttcgcg
    15661 ggctggtgat aactgtgccc gcgtttcata ctttaatttc cctgtacaaa atacaatctc
    15721 cccgggatcg gagaattccc cccccaaaat attcaccgta gccatatgtc atgagagttt
    15781 atcgttctca agatgctcga acgaacgttc gattgcttat tttatggctt ctgtcaacgc
    15841 tgttttaaag attaatgcga tccatatcac gctgtgggta ttgcagtttt tggttttttg
    15901 atcgcggtgt cagttctttt tatttccatt tctcttccat gggtttctca cagataactg
    15961 tgtgcaacac agaattggtt aactaatcag attaaaggtt gaccagtatt attatcttaa
    16021 tgaggagtcc cttatgttac gtcctgtaga aaccccaacc cgtgaaatca aaaaactcga
    16081 cggcctgtgg gcattcagtc tggatcgcga aaactgcgga attgatcagc gttggtggga
    16141 aagcgcgtta caagaaagcc gggcaattgc tgtgccaggc agttttaacg atcagttcgc
    16201 cgatgcagat attcgtaatt atgtgggcaa cgtctggtat cagcgcgaag tctttatacc
    16261 gaaaggttgg gcaggccagc gtatcgtgct gcgtttcgat gcggtcactc attacggcaa
    16321 agtgtgggta aataatcagg aagtgatgga gcatcagggc ggctatacac cgtttgaagc
    16381 cgatgtcacg ccgtatgtta ttgccgggaa aagtgtgcgt atcaccgttt gtgtgaacaa
    16441 cgaactgaac tggcagacta tcccgccggg aatggtgatt accgacgaaa acggcaagaa
    16501 aaagcagtct tacttccatg atttctttaa ttatgccggg atccatcgca gcgtaatgct
    16561 ctacaccacg ccgaacacct gggtggacga tatcaccgtg gtgacgcatg tcgcgcaaga
    16621 ctgtaaccac gcgtctgttg actggcaggt ggtggccaat ggtgatgtca gcgttgaact
    16681 gcgtgatgcg gatcaacagg tggttgcaac tggacaaggc actagcggga ctttgcaagt
    16741 ggtgaatcca cacctctggc aaccgggtga aggttatctc tatgaactgt gcgtcacagc
    16801 caaaagccag acagagtgtg atatctaccc gcttcgcgtc ggcatccggt cagtggcagt
    16861 gaagggcgaa cagttcctga ttaaccacaa accgttctac tttactggct ttggtcgtca
    16921 tgaagatgct gacttgcgtg gcaaaggatt cgataacgtg ctgatggtgc acgaccacgc
    16981 attaatggac tggattgggg ccaactccta ccgtacctcg cattaccctt acgctgaaga
    17041 gatgctcgac tgggcagatg aacatggcat cgtggtgatt gatgaaactg ctgctgtcgg
    17101 ctttaacctc tctttaggca ttggtttcga agcggccaac aagccgaaag aactgtacag
    17161 cgaagaggca gtcaacgggg aaactcagca ggcgcactta caggcgatta aagagctgat
    17221 agcgcgtgac aaaaaccacc caagcgtggt gatgtggagt attgccaacg aaccggatac
    17281 ccgtccgcaa ggtgcacgag aatatttcgc gccactggcg gaagcaacgc gtaaactcga
    17341 cccgacgcgt ccgatcacct gcgtcaatgt aatgttctgc gacgctcaca ccgataccat
    17401 cagcgatctc tttgatgtgc tgtgccttaa ccgttattac ggttggtatg tccaaagcgg
    17461 cgatttggaa acggcagaga aggtactgga aaaagaactt ctggcctggc aggagaaact
    17521 gcatcagccg attatcatca ccgaatacgg cgtggatacg ttagccgggc tacactcaat
    17581 gtacaccgac atgtggagtg aagagtatca gtgtgcatgg ctggatatgt atcaccgcgt
    17641 ctttgatcgc gtcagcgccg tcgtcggtga acaggtatgg aatttcgccg attttgcgac
    17701 ctcgcaaggc atattgcgcg ttggaggtaa caagaaaggg atcttcactc gcgaccgcaa
    17761 accgaagtcg gcggcttttc tgctgcaaaa acgctggact ggcatgaact tcggtgaaaa
    17821 accgcagcag ggaggcaaac aatgaatcaa caactctcct ggcgcaccat cgtcggctac
    17881 agcctcggtg acgtcgccaa taacttcgcc ttcgcaatgg gggcgctctt cctgttgagt
    17941 tactacaccg acgtcgctgg cgtcggtgcc gctgcggcgg gcaccatgct gttactggtg
    18001 cgggtattcg atgccttcgc cgacgtcttt gccggacgag tggtggacag tgtgaatacc
    18061 cgctggggaa aattccgccc gtttttactc ttcggtactg cgccgttaat gatcttcagc
    18121 gtgctggtat tctgggtgcc gaccgactgg agccatggta gcaaagtggt gtatgcatat
    18181 ttgacctaca tgggcctcgg gctttgctac agcctggtga atattcctta tggttcactt
    18241 gctaccgcga tgacccaaca accacaatcc cgcgcccgtc tgggcgcggc tcgcgggatt
    18301 gccgcttcat taacctttgt ctgcctggca tttctgatag gaccgagcat taagaactcc
    18361 agcccggaag agatggtgtc ggtataccat ttctggacga ttgtgctggc gattgccgga
    18421 atggtgcttt acttcatctg cttcaaatcg acgcgtgaga atgtggtacg tatcgtggcg
    18481 cagccgtcat tgaagatcag tctgcaaacc ctgaaacgga atcgcccgct gtttatgttg
    18541 tgcatcggtg cgctgtgtgt gctgatttcg accttcgcgg ttagcgcctc gtcgttgttc
    18601 tacgtgcgct atgtgttaaa tgataccggg ctgtttactg tgctggtact ggtgcaaaac
    18661 ctggtcggta ctgtggcatc ggcaccgctg gtgccgggga tggtcgcgag gatcggtaaa
    18721 aagaatacct tcctgattgg cgctttgctg ggaacctgcg gttatctgct attcttctgg
    18781 gtttccgtct ggtcactgcc ggtggcgttg gttgcgttgg ccatcgcttc aattggtcag
    18841 ggcgttacta tgactgtgat gtgggcactg gaagctgata ccgtagaata cggtgaatac
    18901 ctgaccggcg tgcgaattga agggctcacc tattcactat tctcatttac ccgtaaatgc
    18961 ggtcaggcaa tcggaggttc aattcctgcc tttattttgg gattaagcgg atatatcgcc
    19021 aatcaggtgc aaacgccgga agttattatg ggcatccgca catcaattgc cttagtacct
    19081 tgcggattta tgctactggc attcgttatt atctggtttt atccgctcac ggataaaaaa
    19141 ttcaaagaaa tcgtggttga aattgataat cgtaaaaaaa tgcagcagca attaatcagc
    19201 gatatcacta attaatattc aataaaaata atcagaacat caaaggtgca actatgagaa
    19261 aaatagtggc catggccgtt atttgcctga cggctgcctc tggccttacc tctgcttacg
    19321 cggcgcaact ggctgacgat gaagcgggac tacgcatcag actgaaaaac gaattgcgca
    19381 gggcggataa gcccagtgct ggcgcgggaa gagatattta cgcatgggta cagggaggat
    19441 tgctcgattt caatagtggt tattattcca atattattgg cgttgaaggc ggagcgtatt
    19501 atgtttataa attaggtgct cgtgctgata tgagtacccg gtggtatctt gatggtgata
    19561 aaagttttgg ctttgccctg ggggcagtaa aaataaaacc cagtgaaaat agcctgctta
    19621 aattaggtcg cttcgggacg gattatagtt acggtagctt accttatcgt attccgttaa
    19681 tggctggcag ttcgcaacgt acattaccga cagtttctga aggagcatta ggttattggg
    19741 cgttaacgcc aaatattgat ctgtggggaa tgtggcgttc acgagtattt ttatggaccg
    19801 attcaacaac cggtattcgt gatgaagggg tgtataacag ccagacggga aaatacgata
    19861 aacatcgcgc acgttctttt ttagccgcca gttggcatga tgataccagt cgctattctc
    19921 tgggggcatc ggtacagaaa gatgtttcca atcagataca aagcattctc gagaaaagca
    19981 taccgctcga cccgaattat acgttgaaag gggagttgct cggcttttac gcgcagctcg
    20041 aaggtttaag tcgtaatacc agccagccca atgaaacggc gttggttagt ggacaattga
    20101 cctggaatgc gccgtgggga agcgtatttg gcagtggtgg ttatttgcgc catgcaatga
    20161 atggtgccgt ggtggatacc gacattggct atcccttttc attaagtctt gatcgtaacc
    20221 gtgaaggaat gcagtcctgg caattgggcg tcaactatcg tttaacgccg caatttacac
    20281 tgacatttgc accgattgtg actcgcggct atgaatccag taaacgagat gtgcggattg
    20341 aaggcacggg tatcttaggt ggtatgaact atcgggtcag cgaagggccg ttacaaggga
    20401 tgaatttctt tcttgctgct gataaagggc gggaaaagcg cgatggcagt gcgctgggcg
    20461 atcgcctgaa ttactgggat gtgaaaatga gtattcagta tgactttatg ctgaagtaag
    20521 aaataacgcc ggagagaaaa acctccggcg ttttggctgt gaattactgc tgcggaatag
    20581 cgggtacagc cggaacgttt aatgccggca tcgcaaacat cccaacaaaa tcttccagtg
    20641 gcattttctg cccgtttaac gtgatctgac cgttagcata ctgcaggctg gtggtgatgg
    20701 tattgtcctg caaggtggtc agacggaaca tctgccccat tgctgatgca ccttcaactt
    20761 gctgtttcgc cagttttttc gcttgatctt cctgataacc ttccagcttc gctacctgag
    20821 tcataaactc agttgccata tccaccggaa tggtcagttt cgcatccaga gatttaaccg
    20881 aacgatctac ttcctgcgcc agcgtttgcg gcgcttcttt agtcgttgcc ggatctttca
    20941 ggaacagcga cagattcagc gcactttcac cctgactgtt tttccagctt agcggcgcaa
    21001 tagtaatcac cggatcgcct ttcagcatca gcggcagggc gctaaagaag gcttccgtca
    21061 ctttctcctg ataaagttcg ggattgttgg caatttctgg ctgtgccagc agcgcctgag
    21121 tttgcgcgtt atattgctgg ctaaactgat gccaggcttc gccatcaatt tggccgactt
    21181 ttaaagtcag cttgccgctg cccagatcct gattctgtac cttcaggctg tttagcgagt
    21241 aatccagttg gctattgatc gttttaccat cattgaccag atccgattta ccgctgatct
    21301 ccatgccttc cagcagtgcc agttctttgc cttccactga aatggtcatt ttttccagtg
    21361 acagtttttg gtttcctacg cgctcaccaa aacttgccag cgtgctggaa ccgtcggttt
    21421 tcagattatt aaaggtcaac tgcactttct gattgtattc gttaactgcg tctatccgac
    21481 cactttgcgc ttccccggaa agggagatag ctttgccgtc tctgtcagca tttaactgga
    21541 actcgccgcc gctaaaggcg actttttcat ccttttgctc gtaattcagt ggattgagcg
    21601 aaatatcgga actggaatca ccgctgtaac caatgcggga gttaatctca aaaggcgttt
    21661 caccttttgc catatcaaac agtggtttgc ttacttcgtt attgaccagc gtggtttgaa
    21721 ttgatgccat cgacgggatc aggttcagtt ttttaagctg ggcaaggggg aagggaccat
    21781 gatcaaccga ttcgttgaag atgacgctct gaccgctttt aatccacgga ttttctttcc
    21841 cggcaatggg tttcaccaac agttgcaact ggctgctgaa tacgccgcga tgatagtttt
    21901 gataactcac ttccaggttg gattcaggag ctgtcagttt gagttgcgcg ttcgcctgcg
    21961 cgaccatgtc ttcgagatgg gtttcaatct tcttgcctgt ataccatgcg ccgcctgtcc
    22021 agactacgcc tagcgcaaca atgacgccta ccgctaccag cgatttattc ataatgatta
    22081 tccataaaat gaaatcaggc ggactggccg cctgaaggtg ttataagcct ttaataagct
    22141 tagcaagaga tgttaatttt ttcagtaagc tcttacagct tgttgtaaac acgcgctaaa
    22201 cggccgtggc ctttgacagt caccggtgat tcgttggcgg caataaacgc tgattcaccc
    22261 ggtttaagct gtaactgctg agaaccttga cacagcgttg catcgccttc gacgcagaac
    22321 aaaatggcgg cactttgctg gctaatggtg gtttctttat cactaagatc atgcagcgag
    22381 aaggcaaaat catccacagg aatcgagaag tccagttctg caccttgttt caccggctgg
    22441 gtcaacaact ggttagccgg tttggcttcg aacttcacat tggcaaccag ttccggaata
    22501 tcaatgtatt taggcgtcag acccgcacgc agcacgttat cggagttcgc catcacttca
    22561 agcgccacgc cttgcaggta agcgtgcggt gtttcagcga acaggaacat cgcttcgcca
    22621 ggattcaatt tcaccacatt cagcaatagc ggggagaaca gaccgctgtc ttccgggtaa
    22681 aattcagaaa ttaaacgaat cgtttgccac ggttcaccct gctggctatc gagggccgat
    22741 tttaaaatcg ccagcgcgcg ggatttttct tcaccctgca tattcaacag gctggcgaac
    22801 agttcgctta aacgttcagc atttggctgt tgtaaaaagt gagcaatcgc cggatgtgca
    22861 cctgcgaccg gctggagtag ggagacaatc tcggaaaatt cacgaaacgc gttcatcgca
    22921 aggaaaggcg tcagcgcaaa aaccagctcc ggcttgtggt taggatcttt atagttacgc
    22981 tcggcggcat ccatcgggat acctgcggca ttttctttgg caaaaccgat ttcagaattg
    23041 tgtttgtttg gatgaacctg aatggagagc ggctgtgctg cgcataatac tttgaacagg
    23101 aaaggcagtt cgccaaagcg tttggcaacg gcctctccga gcagagtcga tttatcactc
    23161 tcaatcacat cacgcagtga aacgatatct ccggcggcat tctgcactcg tgaactgctt
    23221 ttcggatgtg cgcccatcca cagctcggcc atcggctggc tggacggatt ttccatacca
    23281 taaagttcag tcaacgccgt tttgctgccc caggcatagt tttgcactga gttaatgagt
    23341 ttttgcatga tcaatccctg ttttaatgtg gaaattaatc ccgctattaa agcaagaatc
    23401 ctacgggaag taacctggag ccgcaaaaag tcgtactagt ctcagttttt gttaaaaaag
    23461 tgtgtaggat attgttactc gcttttaaca gggcaacgga acacccgccc agagcataac
    23521 caaaccaggc agtaagtgag agaataatgt caaacaaacc ctttcattat caggctcctt
    23581 tcccactcaa aaaagatgat actgagtatt acctgctaac cagcgaacac gttagcgtat
    23641 ctgaatttga agggcaggag attttgaaag tcgcacccga agcgttaact ctgttggcgc
    23701 gtcaggcgtt tcatgatgcg tcatttatgc tgcgtccggc tcaccaacaa caggtggccg
    23761 acattctgcg tgacccggaa gccagcgaaa atgataaata tgtggcgctg caattcctgc
    23821 gcaactccga catcgcggcg aaaggcgttc tgccaacctg tcaggatacc ggcaccgcga
    23881 ttattgttgg taaaaaaggg cagcgtgtat ggaccggtgg cggtgatgaa gcggcgctgg
    23941 cacgcggtgt ctataacact tatatcgaag ataatctgcg ctactcgcaa aacgcgccgc
    24001 tggatatgta taaagaggtg aataccggca ccaatctacc agcgcagatc gatctttatg
    24061 ccgttgatgg cgacgagtac aaattcctct gtatcgccaa aggtggcggt tcggcaaaca
    24121 agacgtatct ctatcaggaa accaaagcgt tactaacgcc ggggaaactg aaaaattacc
    24181 tggttgagaa gatgcgcacg ctgggtacgg cggcctgtcc tccgtatcat attgcgttcg
    24241 ttattggtgg aacttctgca gaaacgaacc ttaaaacagt gaaactggct tccgctaaat
    24301 actatgacga actgccaacg gaagggaatg agcacggtca ggcattccgc gatgtggaac
    24361 tggaaaaaga attgctgatc gaagcgcaaa atctcggtct gggtgcgcag tttggtggta
    24421 aatacttcgc tcacgacatc cgcgtgattc gcctgccacg tcacggcgca tcctgcccgg
    24481 tcggtatggg cgtctcctgc tctgctgacc gtaatatcaa agcgaagatc aaccgtcagg
    24541 ggatctggat cgaaaaactg gaacataatc caggcaaata tatcccggaa gagctgcgca
    24601 aagcgggaga aggcgaagcg gtgcgcgttg accttaaccg tccgatgaaa gagatcctcg
    24661 cacagttgtc gcagtatccc gtttctacac gcttatcgct taacggcacg attatcgtcg
    24721 gtcgtgatat tgctcacgcc aaactgaaag agcggatgga taatggtgaa gggctgccgc
    24781 agtacattaa agatcatccg atttactacg cgggtccggc caaaacgccg gaaggttatg
    24841 cttccggttc tcttggccca acgaccgccg gacggatgga ttcttatgtc gatcaactgc
    24901 aagcgcaggg cggaagtatg atcatgctgg cgaaaggcaa ccgcagccag caggtgacgg
    24961 atgcctgtaa aaaacacggc ggcttctacc tcggcagtat cggtggtccg gccgctgtat
    25021 tggcgcaggg aagtattaag agcctggaat gtgttgaata tccggaactg ggaatggaag
    25081 ccatctggaa aattgaagtg gaagatttcc cggcgtttat ccttgtggat gataaaggaa
    25141 atgacttctt ccagcagata caactcacac aatgcacccg ctgtgtgaaa taaacagagc
    25201 cgcccttcgg ggcggttttt ttacatggca cgaaagacca aacatttgtt atcaaatagt
    25261 aaataatgag tgagctaaaa gttgcttaac gaaagcaaaa cagaaagaaa aaattaaaca
    25321 ggtgaggagc aggccatgaa tacagtacgc agcgaaaaag attcgatggg ggcgattgat
    25381 gtcccggcag ataagctgtg gggcgcacaa actcaacgct cgctggagca tttccgcatt
    25441 tcgacggaga aaatgcccac ctcactgatt catgcgctgg cgctaaccaa gcgcgcagcg
    25501 gcaaaagtta atgaagattt aggcttgttg tctgaagaga aagcgagcgc cattcggcag
    25561 gcggcggatg aagtactggc aggacagcat gacgacgaat tcccgctggc tatctggcag
    25621 accggctccg gcacgcaaag taacatgaac atgaacgaag tgctggctaa ccgggccagt
    25681 gaattactcg gcggcgtgcg cgggatggaa cgtaaagttc accctaacga cgacgtgaac
    25741 aaaagccaaa gttccaacga tgtctttccg acggcgatgc acgttgcggc gctgctggcg
    25801 ctgcgcaagc aactcattcc gcagcttaaa accctgacac agacactgag tgaaaaatcg
    25861 cgtgcatttg ccgatatcgt caaaatcggt cgaacccact tgcaggacgc cacgccgcta
    25921 acactagggc aggagatttc cggctgggta gcgatgctcg agcataatct caaacatatc
    25981 gaatacagcc tgcctcacgt agcggaactg gctcttggcg gtacagcggt gggtactgga
    26041 ctaaataccc atccggagta tgcgcgtcgc gtagcagatg aactggcagt cattacctgt
    26101 gcaccgtttg ttaccgcgcc gaacaaattt gaagcgcttg cgacctgtga tgccctggtt
    26161 caggcgcacg gcgcgttgaa agggttggct gcgtcactga tgaaaatcgc caatgatgtc
    26221 cgctggctgg cctctggccc gcgctgcgga attggtgaaa tctcaatccc ggaaaatgag
    26281 ccgggcagct caatcatgcc ggggaaagtg aacccaaccc agtgtgaagc attaaccatg
    26341 ctctgctgtc aggtaatggg gaacgacgtg gcgatcaaca tgggtggcgc ttccggtaac
    26401 tttgaactga acgtcttccg tccgatggtg atccataatt tcctgcaatc ggtgcgcttg
    26461 ctggcagatg gcatggaaag ttttaacaaa cactgcgcag tgggtattga accgaatcgt
    26521 gagcgaatca atcaattact caatgaatcg ctgatgctgg tgactgcgct taacacccac
    26581 attggttatg acaaagccgc ggagatcgcc aaaaaagcgc ataaagaagg gctgacctta
    26641 aaagctgcgg cccttgcgct ggggtatctt agcgaagccg agtttgacag ctgggtacgg
    26701 ccagaacaga tggtcggcag tatgaaagcc gggcgttaat ctgcaacata caggtgcagc
    26761 cgtggaatga tcaaacgaag cggctgcgcc tgtggtttat aacggtgctg cacattgtcg
    26821 gcatcgtaat ttaacaactc accaacgtct ggcacgcccg caccattatc ccgattaatc
    26881 agtgcaatca gcggtgtagg gcaggcgtgt tgcacttgtt tttgatctcc tttgtaccag
    26941 acgcgggcaa tcggctgcac cttcaccgga cgtttgattt ttaacttcgc gttctgtggc
    27001 agggcagcga tatcctgata ctctcgctcc agttttcttt gccactcctc gcgcgtccac
    27061 ggtgcgacac tgcgtggtga tttcaggctt ttttccagct gtgccaggac ttcatcacga
    27121 tgcaaattct taatgatatg tttattagcc caaccaaagc gtaaagtggc ggggtcgtgc
    27181 agaacggtga gcgtgcggta agcattaagg gtgatcagcc ccggcaaatg acgatgcacc
    27241 cattcaaaac gtgccgcggt ggggagttct gattcaaccg tgacgatatg ctcgaacgtg
    27301 gtcttgagtt tattgatgtg ctgaatatga ctgaccaacg ctgcttgcga aaggttatcg
    27361 acctggtaac acaacacgcc aggcagacgg acagcggcct tgctgctgcg attttcggac
    27421 tgttgttgaa taaataaatg gcggaaatga cgcaacgcca gcgactgcgc gtcgttgccg
    27481 agatgttgtt ttacctcaat acgattaagc ggattatgct catcctcttt ttttacctcc
    27541 ggcaaagaga acacgcgggc aaccaatagc ttgtgttgct caagatgagc ggcaaatgca
    27601 gccagctctt gttccatctg gcgaaaggta gtgttgagtc ggtctacgag atcgtaacgc
    27661 gccataatat taaccacttt agttacaaca tccttaattt atagcacaat ggtggtgact
    27721 tgaccatcgt gcgcgtagtg tcaggcagag gtaaattgcg ggatgttatg ccataacggc
    27781 cagctaaacg agaagcgagc accacccagt tcgctggtgt cacaattaac cgtaccgccc
    27841 attgccagtg ctatagagtg gacaattgcc agccccagcc cgcagccgcc ggttgagcga
    27901 tcccggctgg gatcgaggcg aacaaaaggt tcaaagatat gttcgcggtt ttctggggca
    27961 atccctgggc catcatcctc aacaattaat gtcgctctat tccccgacag tagcaggctg
    28021 gtttcaaccg ttgaatggca gtaccgcagg gcgttattga gcaaattatc cagcacgcgc
    28081 tccattaagc gcatatccaa cgccgcataa tggccttgca cgagcgtttt tatccgtacc
    28141 gttttatcgg gcgttactgc ctgaatatct gccagatgcg ttgacagcca caacggcagg
    28201 tctggttcgc taagatgaag ctcgttttgt gggcgatcga gtcgggcata agtcagcagc
    28261 tcttcaatta aagcttcaag ttgactgata tcacgattca acgcctggga ttcggcggcg
    28321 ctcaggttat cgctcatctc cagtcgataa cgcaggcgca ctaacggtgt tcgcagttcg
    28381 tgagcgatac cgtcaataag ctgttttttg ctggcaatca aggcgttgat attgtccgcc
    28441 atctggttaa atgcgatacc aagtcgttca aagctcgaac cctcatcaaa gtggatacgc
    28501 tcattgagat gcccatcgcc aaatcgttgc gccgctgctt ccagttttaa catatcctgc
    28561 cagtgcggac gcatccagat aaacaccgga aaggcgaggg aaatagcaat aaaagcgatc
    28621 agggcgatat ccagcaatcg catctgatgg agataataaa gataaggaac aggaccaact
    28681 gccagcacgt agtggctgcg cgggatacgc tgcaaaaacg tgtactgatc gtccagggcg
    28741 acaatttcgc cgccacgcag tcggtgcatg gaaatatcat caagatggta tttactcagt
    28801 ggctcgacac gcagatcgaa agagagattt aaatccatct ccttcagcgt cttaccccaa
    28861 tcgtgtgggg ggatctcacg caattcgcta cgcatcagat acagcgaact gttcatcaaa
    28921 tcatccagcg actgtttgcc cgcgcgttcg gcggtaaatt tgtacaccag cccaaccagc
    28981 agagacatca caaggaagca gacaaacaat aacaggtaaa actggataaa cagttttttc
    29041 atcgcttatt cccatgcatg aggcgcaaaa agatagcctt tgttacgcac agttttaatg
    29101 cgataaggtt ctgcggcgtt atcgagcagt ttttttctta accgcgaaat agccacgtcc
    29161 acgctacgat ccagtccgtc ataactgacg ccgcgtaaat ttttcagcaa tgcatcgcgg
    29221 tccatgattt gcccggcatg ggtagctaat tcccacaata attcgaaatc agctgtcgag
    29281 agcgagattt cagtgttagc cagggtgact acgcggttga tgggatcgat ggtcaacgtg
    29341 ccgaaatgca gggctttgta gggagtcaga gacgtttcct gaagaccttt ggtcagtgtg
    29401 gcttgctcat tctgacgcaa atgcaaacgt aaacgcgcta gcaaaacagc agggggcgtc
    29461 gttttgagaa tatagtcgca ggcacccatt tccagtgcca ggatgtggtt catatcgcta
    29521 tcgagagagg ttagaagaac aatcggtcca gaccactttg cgcgtaaatc acgacaaatg
    29581 gtcatgccgt ccttgcctgg tagcatgatg tcgagtaaca ccaaatccgg attttctcgc
    29641 aaaatggttt cttcggcctg gtcgccgcgc ggctctacgg taacctgcat atcatgtttt
    29701 gccaggtacg cggcaatcag tgaaccgact tccgcatcat cttccacaaa tacgatagtg
    29761 ttcatattat tcacggtaga tataaaaacg tcacaataca ccgcgctgtt tttacttact
    29821 atcaatcgtt tctaaacaag ttttcatcag gtattctact gatgacttgt tatggaagtg
    29881 ttaaggtaaa aaaatggggc tggtaatcaa agccgcgctc ggtgcgctgg tagtgctgtt
    29941 gattggtgtt ttagcaaaaa cgaaaaatta ttatatcgcc gggctgattc cacttttccc
    30001 gacctttgcg cttatcgcgc attatattgt tgccagcgaa cgcggcattg aagccttacg
    30061 cgcaaccatc atttttagta tgtggtcgat tattccctat tttgtttacc tggtgtcgct
    30121 gtggtatttc accggaatga tgcgcttgcc cgccgctttt gttggttctg ttgcgtgttg
    30181 ggggataagc gcatgggtgt tgattatatg ctggataaag ctgcattaac gcagatgacg
    30241 accgccatca agtgggaaac tgcgtccggt gacaaagcaa ctggtaagta agtaatcgac
    30301 caggtcgatc acttctttct cgccaggcgc ggttttcatc agtgatttat tcagcgcctg
    30361 ttgtcgatat tcggcatcat catgttcatt aaacaggatc agcgatggcg caatagaatt
    30421 cactttcact tccggtgcca gcttgcgggc aaacgagcgg gtcatattat ccagtgccgc
    30481 tttgcttgca gcatacgcaa catgtttgtc gctaccgcgc tccaccacat aatcggtaaa
    30541 gtgaatgata tcgctggcgg cgtgtccgtg cccacgcagt aatctttcca gcgcatggtt
    30601 gagcaggtat ggggtattaa cgtggatctg catcatgcaa gccagtacgt cggccagtgg
    30661 cgcacccggt ttttccgcca tccacgcact ggcgttatgc aaaatagcac gcagaccatg
    30721 ggtgcttttt agtacttcat cggcaaacgc catcacaccg tcgttggtcg aaaaatcagc
    30781 ctgaatacac tgcgcacctg cattaatcag tccatcaatg gctggatagt gtgtccgata
    30841 gctgacaatc accggttgct tttgattaat gaaatgccat gcgagggcga ggccgatgcg
    30901 acgaccaccg ccagtaatta atattggcaa gggctgggct ttacccatcg ttatctcctt
    30961 tgctaaccaa cgatgggatg aacgttatcc caccagcatc caggtcgccg gaactgaggc
    31021 aattaacagc atgccaatta gcaccatttc ctggcggttc agtacgttat catgtgtatg
    31081 cgttttacgc gcatacagga aaaccagcag ccccggcgca tacagaacaa cggataacaa
    31141 caagtgcatt ggaccagagg catacaataa ccataagcca taaatgcagg caccgacacc
    31201 taccgcttta tgcagtggag gggttgcgat tttcagcaag aacgcgccga ccaggaaata
    31261 gggcaccaga atcatttctg aggcgatggt cagcaacgtg ttgtaatcgg aaccggtcag
    31321 ccagatgagt accagacaaa tttgcacgca gatgttggtc aaccacagcg aggcggatgg
    31381 cgcagcttgt gcgttctggc gcgcaaaaat gcgtgggaat gctttatgag tggcagccag
    31441 gaacggaact tctgccgcca tgatggtcca gcttaagtac gcgccgcaaa cggaaacgat
    31501 caaaccggca gcgatgatga tttcgcccca tggtcccatc atttccacca tcagaccggc
    31561 cattgacggg ttacgaattt cagccagttc aggacgtgcc accacgccca gcgaaagcag
    31621 cgttaccagc aagtaaacgc ccagagcgga gagaaccgcc agcagtgtcg ctttgccaac
    31681 atcacgttta ttacgcgcac gcgcagaaac aaccacggca ccttccacac caatgaaaac
    31741 ccacagggtg atcagcatgg tgtttttcac ctgttcccac acgggtacgc caagtgcaag
    31801 tccggtgaag tcgagcttga aggtatccag tttgaacatc atcatcgcca gcacaacaaa
    31861 cagacccagc ggcaacaatt ttgccagtgt cgccaccagg ttaatgctgg cagcggtttg
    31921 caccccgcgc aggatcaaaa agtgaacaat ccacaataaa gccgatgcac cgactatcga
    31981 ttgccaggta ttaccatcgc cgaacaggcg caattccggc gtgtccgtaa aaaagcttaa
    32041 cgcggaaaaa acgatcacca gataggagac gttggcgatg actgcgcaca gccagtatcc
    32101 ccatgcggaa caaaagccga ttagctcgcc aaacccttcg cgggcatagg taaagatacc
    32161 gccgtcaagt tcgggacgaa tgcgcgtgag gatcagcatg gcaaaggcca gcaataaaat
    32221 gccagcgcca gtaatacccc agccgatgag cagtgctgcc gggctggcaa ctgccgccat
    32281 attttgcggc agactgaaaa cacccgcgcc cagcattgag cttaatacca gcgcggtgag
    32341 tgcgctcagt cccagtttct tttccatcga tatccagtga ttaaaacgtg ataagaagaa
    32401 taaatattag ggcaaaacgc ataaaaatgg agtttgccac tgaagcgcgg gattttacga
    32461 agagatgtgt ctgcatgcaa tggtcgcggg gaaatttgtg actaaaaatg cgaaaggcgg
    32521 catcaagccg ccttatagga gtaactgaat agttatttat acagatctgc gctgacggtc
    32581 aggttattac cacgttcctg ccactggcgg gtgatgtggt aatacttagc acctttcttc
    32641 gcggcacgtt tcgcaacctg ataggagact tcggtcatgt tgccgtagtt gccagagaat
    32701 ttgatgctgt cgaacgggac catcatcgct gcggtcgctt tgttcagttc ttcgacttta
    32761 gtgccatccg ggagcgtgac ggtgtaacgc ccgccttttg atgactgggt ttcaaagaag
    32821 cgaccgactt cagaacttgg tgatgcggta gtcgcaacac ccgggatctc aactttcttc
    32881 gcggcttcgc caccggcagc cagagctgca cgtcctgctt cggaatctgc cgggatcaca
    32941 tccgggctct ggacgatacg tttcttagca tcttttttat agatgaatgc agtaatacgc
    33001 tggttgccgc cctggttggc atcgatttga cgaacgatgt agaaagagta ggcacctttc
    33061 gcttttgctg ctttggtgat ggcagcattg acttctggct ggctacgata gaagccctgg
    33121 acggtgaccg tgtcaaacgg ttcaatcaga acagcctgat ctttcggcag ttcgacaaca
    33181 ccgttaatta cgcgattact tgtttcttct gctttttcag catcggcttt atagaggtca
    33241 gcgaccacac gccagttacc gctgttacca aaatcagaag tgtcgacaac ataaaaagag
    33301 gcggcacctt ctttatctgc gcgacgagaa acggctttca ccgcttcgcc aatagcatta
    33361 aaacgaccgg taaccactac acggtcaaaa ggtttaaccg ctgccgcttg ctccggtgtc
    33421 agttctgttg ctgcgttaac ggagaatgcc gtagcagaaa gcagtgccga cgccaggagg
    33481 gtgttcttaa gcttcataaa aataatcctt cgccttgcgc aaaccaagta ctggtattgt
    33541 tattaacgag aaacgtggct gattattgca tttaaacggt gtaactgtct gcgtcatttt
    33601 tcatatcaca ttccttaagc caattttaat cctgctcaaa tgaccgtcta tgcttaaaaa
    33661 acagccgtat cagcatcatt actactgaag caactgaatt gtataagtta atttaatgtt
    33721 aagtagtgat gcgtgccggg gcgatgtctc gttttacccg accgtcgaag acaattatca
    33781 gtctttatcc ggcgttctaa ggtgtttatc ccactatcac ggctgaatcg ttaatatttt
    33841 gcgagttcac gccgaaatac tgatttttgg cgctagatca caggcataat tttcagtacg
    33901 ttatagggcg tttgttacta atttatttta acggagtaac atttagctcg tacatgagcg
    33961 gcttgtgtgg ctcctgacac aggcaaacca tcatcaataa aaccgatgga agggaatatc
    34021 atgcgaattg gcataccaag agaacggtta accaatgaaa cccgtgttgc agcaacgcca
    34081 aaaacagtgg aacagctgct gaaactgggt tttaccgtcg cggtagagag cggcgcgggt
    34141 caactggcaa gttttgacga taaagcgttt gtgcaagcgg gcgctgaaat tgtagaaggg
    34201 aatagcgtct ggcagtcaga gatcattctg aaggtcaatg cgccgttaga tgatgaaatt
    34261 gcgttactga atcctgggac aacgctggtg agttttatct ggcctgcgca gaatccggaa
    34321 ttaatgcaaa aacttgcgga acgtaacgtg accgtgatgg cgatggactc tgtgccgcgt
    34381 atctcacgcg cacaatcgct ggacgcacta agctcgatgg cgaacatcgc cggttatcgc
    34441 gccattgttg aagcggcaca tgaatttggg cgcttcttta ccgggcaaat tactgcggcc
    34501 gggaaagtgc caccggcaaa agtgatggtg attggtgcgg gtgttgcagg tctggccgcc
    34561 attggcgcag caaacagtct cggcgcgatt gtgcgtgcat tcgacacccg cccggaagtg
    34621 aaagaacaag ttcaaagtat gggcgcggaa ttcctcgagc tggattttaa agaggaagcg
    34681 ggcagcggcg atggctatgc caaagtgatg tcggacgcgt tcatcaaagc ggaaatggaa
    34741 ctctttgccg cccaggcaaa agaggtcgat atcattgtca ccaccgcgct tattccaggc
    34801 aaaccagcgc cgaagctaat tacccgtgaa atggttgact ccatgaaggc gggcagtgtg
    34861 attgtcgacc tggcagccca aaacggcggc aactgtgaat acaccgtgcc gggtgaaatc
    34921 ttcactacgg aaaatggtgt caaagtgatt ggttataccg atcttccggg ccgtctgccg
    34981 acgcaatcct cacagcttta cggtactaac ctcgttaatc tgctgaaact gttgtgcaaa
    35041 gagaaagacg gcaatatcac tgttgatttt gatgatgtgg tgattcgcgg cgtgaccgtg
    35101 atccgtgcgg gcgaaattac ctggccggca ccgccgattc aggtatcagc tcagccgcag
    35161 gcggcacaaa aagcggcacc ggaagtgaaa actgaggaaa aatgtgcctg ctcaccgtgg
    35221 cgtaaatacg cattgatggc cctggcaatc atccttttcg gttggctggc aagcgttgcg
    35281 ccgaaagagt ttcttgggca cttcaccgtt ttcgcgctgg cctgcgttgt cggttattac
    35341 gtggtgtgga atgtatcgca tgcgctgcat acaccgttga tgtcggtcac caacgcgatt
    35401 tcagggatta ttgttgtcgg agcactgttg cagattggcc agggcggctg ggttagcttc
    35461 cttagtttta tcgcggtgct tatagccagc attaatattt tcggtggctt caccgtgact
    35521 cagcgcatgc tgaaaatgtt ccgcaaaaat taaggggtaa catatgtctg gaggattagt
    35581 tacagctgca tacattgttg ccgcgatcct gtttatcttc agtctggccg gtctttcgaa
    35641 acatgaaacg tctcgccagg gtaacaactt cggtatcgcc gggatggcga ttgcgttaat
    35701 cgcaaccatt tttggaccgg atacgggtaa tgttggctgg atcttgctgg cgatggtcat
    35761 tggtggggca attggtatcc gtctggcgaa gaaagttgaa atgaccgaaa tgccagaact
    35821 ggtggcgatc ctgcatagct tcgtgggtct ggcggcagtg ctggttggct ttaacagcta
    35881 tctgcatcat gacgcgggaa tggcaccgat tctggtcaat attcacctga cggaagtgtt
    35941 cctcggtatc ttcatcgggg cggtaacgtt cacgggttcg gtggtggcgt tcggcaaact
    36001 gtgtggcaag atttcgtcta aaccgttgat gctgccaaac cgtcacaaaa tgaacctggc
    36061 ggctctggtc gtttccttcc tgctgctgat tgtatttgtt cgcacggata gcgtcggcct
    36121 gcaagtgctg gcattgctga taatgaccgc cattgcgctg gtattcggct ggcatttagt
    36181 cgcctccatc ggtggtgcag atatgccagt ggtggtgtcg atgctgaact cgtactccgg
    36241 ctgggcggct gcggctgcgg gctttatgct cagcaacgac ctgctgattg tgaccggtgc
    36301 gctggtcggt tcttcggggg ctatcctttc ttacattatg tgtaaggcga tgaaccgttc
    36361 ctttatcagc gttattgcgg gtggtttcgg caccgacggc tcttctactg gtgatgatca
    36421 ggaagtgggc gagcaccgcg aaatcactgc agaagagaca gcggaactgc tgaaaaactc
    36481 ccattcagtg atcattactc cggggtacgg catggcagtt gcgcaggcgc aatatcctgt
    36541 cgctgaaatt actgagaaat tgcgcgctcg tggtattaat gtgcgtttcg gtatccaccc
    36601 ggttgcgggg cgtttgcctg gacatatgaa cgtattgctg gctgaagcaa aagtaccgta
    36661 tgacatcgtg ctggaaatgg acgagatcaa cgatgacttt gctgataccg ataccgtact
    36721 ggtgattggt gctaacgata cggttaaccc ggcggcgcag gatgatccga agagtcctat
    36781 tgctggtatg cctgtgctgg aagtgtggaa agcgcagaac gtgattgtct ttaaacgttc
    36841 gatgaacact ggctatgctg gtgtgcaaaa cccgctgttc ttcaaggaaa acacccacat
    36901 gctgtttggt gacgccaaag ccagcgtgga tgcaatcctg aaagctctgt aaccctgacg
    36961 gcctctgcgg aggccgtcac tctttattga gatcgcttaa cagaacggcg atgctttgac
    37021 ctcccgctgt ttgttcaagc gcaattttga caataattgt caacggcacg gaaagcagca
    37081 tacccaccgg tcctaacaac catccccaaa aaatcaacga caaaaatacc accaatgtcg
    37141 aaagccccag cccacgtccc atgatgcgcg gctccagaat attgccgaag accagattaa
    37201 tcagcagata tcccgccagc accagcaacg cttcgtagaa gccattaaac accagtacct
    37261 gagcgatagg ggggattgcc gcgagaactg aaccaatatt agggatgtaa ttaagcgcaa
    37321 aggccagcaa tccccagaca aaagcgaagc gaacatcgag tgcggcgagc atcgcccagg
    37381 cgaccaggcc ggtgatgatg ctgatggctg ttttcagcac cagataatga gaaacactgt
    37441 caatcgcacg ttgaatcgcc gccatccctt caaccggacg cgccatcatt tgctgaaatt
    37501 ttccgggcaa ttgtggcact tcgagcagca taaacagcac cgtcagcagc aataaaaata
    37561 ttgatgacat ggcattagat aactgcgtca ataagttggt gagcaacgtc atcgccgcgt
    37621 tcggatcaat gtaatgcgcc agttggtcaa ctgagacgtc aatccctacg cgttgcaaca
    37681 acggctcaag agcttgcagc ggcgtcataa tagagttgcg atattgcggt aacgtccgcg
    37741 tcaactcgtt gagcgcggaa cccagataag ctaacagcaa caccatcgcc atcacgatga
    37801 tggtcatcaa aatcgacacc gccagtacac gcggcactcg ccagcggacc atgtgttgca
    37861 ccagcgggtt aagaataaca gcaataaata atgcgagaat aaacggcacg atgatctcgg
    37921 cggcaaaacg gataccgcag agaataatga ccagcattcc caacataatg acgattttta
    37981 ggccattgag cgtgatgatc ggctttgcca tgcttgtccc cgttttttct tcctttgcta
    38041 ataataatgt ctttttcgct ccgtcatcat aacgaaaatc aaaccgctca ggtaaagaag
    38101 tgaaaatatt ttgagttaat tcttaagcta tgatacaaat caggcgtgtt caactaccga
    38161 ggacaattat catccgcgat gacgagaagc aacactgcgg ataattgtaa tattatggac
    38221 aatttgttca ggacgttatt ttcaacattt acccatcttc gtacctcatc cactatcttg
    38281 ctcgtaggtg agcagcactg gcgcaacgcg ctttagtcct ttcttctgcc taagcttgcc
    38341 ctatgcgcgg ggcttgacca ctttactttg gtttcgctga attaagcgaa aattaaaata
    38401 attctcttgc aggagaagga caatgtatat ttattggatt ttattaggcc tggctattgc
    38461 tacagaaatt accggtacgc tgtcaatgaa atgggcgagc gtcagtgagg gaaatggcgg
    38521 ctttatttta atgctggtga tgatttctct gtcgtatata tttctctctt tcgccgttaa
    38581 aaagatcgcc ttaggcgtag cttatgcgct gtgggaaggt atcggtattt tatttattac
    38641 cttgtttagc gttttgttat tcgacgaaag tttatcgctg atgaaaattg tcgggttaac
    38701 caccctggtc gccgggattg tgttgatcaa atcaggtacc cgtaaagcgc gtaaacctga
    38761 actggaggtg aaccatggcg cagtttgaat gggttcacgc cgcctggctg gcattggcaa
    38821 tcgtgctgga aatcgttgct aacgtctttt tgaaattttc tgacggcttt cgtcgcaaaa
    38881 tatttggctt gctctccctg gcggcggtgc tggctgcctt tagtgcgctt tctcaggccg
    38941 taaaagggat cgacttgtct gtcgcttatg cattgtgggg cgggtttggt attgccgcca
    39001 cgttagccgc aggttggatc ttgtttggtc aacggttaaa tcgtaaaggc tggattggcc
    39061 tggtcttgct gttggctgga atgatcatgg tgaaacttgc ctgatgaaca cgctgcccgc
    39121 actgtcgggc agcgtttgaa cattattttt gcgacagttg atccagcttg tcgcgaaaac
    39181 cggtaacaga aatggcccgg ttatcggcgc gccagcgatc tttcgcggca ggagccgaac
    39241 tttgcacccc aattaattgc cagccgtcat cggtatgcaa catcagaggc gaaccgctgt
    39301 cacccggcaa ggtatcgcac tgatgtgaca tcaccgacgt ttgcgcccag ccagtcactt
    39361 cacagttttg atgactgtat aacgtatcga gatgatcttc aggatatccc gcctgagtca
    39421 ctttacgacc agccgatttt aatgcggcag taagcgcggc tttatctccc tcaaataacg
    39481 gcaacggtgt aatgccagaa gggggattac gcagcacaat caagccgaag tcccatggtg
    39541 cagctgcggg aggaacaatc caaccatccc catctgcttt taaccgcttt cccagtgtcg
    39601 gatcaacgcg gccttctatg tcgtggatgt catagcgcca aagaccttta tttgacacaa
    39661 aacgcagcgc taccgcctta tccgctttac ccttaggcgg cgtcaataaa cagtgtcctg
    39721 ccgttaatgc cagattgggt gcaatcagcg tcgccgtaca taaattgccg ctggccgttt
    39781 ccagttgccc aaccgcatcc cacggtgatt gggtcgtgtc attcactggc acacgatcat
    39841 catgaccaaa aaacagggtg ctgacctcat cgtttgccga tttggcaacg tctggtttat
    39901 ctgcaaacac aaaagcagac gtcaaactaa ttgcacccaa cactacagca atggttgtac
    39961 gcatatcaca ctctggtggg taattatgat tattaaaagc aaaccctcat aaatactata
    40021 gacgggacgg gatgaaagtg ggagtaaaat cagataacta cagtcaggaa agataaaaac
    40081 gggcggcaat cagtgcgcat aaaataagga tgataagaat gaactcaaag cgataacggc
    40141 ccaccatact accctccgct aaaggcggtg ctaaacgtta cccgcttagc accgtaattt
    40201 tcaggcgcga gggggcgcgc cagcattact gttgaaaact tacgctgcgg gttgtgcagc
    40261 aggttttgcc ggttgctgat ggctgtgttt cccggcgtgt ttcttcgctg cctgcgcttt
    40321 ttgttcaggg gctttctgtt cagctttcgt atttttatga tgctttttag ccgcctgcgc
    40381 tttctgggca ggtgctgctt tatgctgttt tttatgatgt gtcgttttcg ccggcgctgc
    40441 tttggtggtc gtcgcagtcg gagcaggtgt ggtcgcagtc tctgcagcaa aggcggcaga
    40501 agacagaccc atagcagcgg caacaaccag agctaatact tttttcattg tcataccctc
    40561 aatttgtttt ttcatttaac cccactgcgg ggccgttgaa ataactatat ccctggaaat
    40621 ggcagacttc cgtgagtggt tggtttcagc gtgtaacgat atgtacaaac gctgaataaa
    40681 ttacagcgtt gataataacg tattgtgact taagggaaat ttagctacca ataatagtag
    40741 tcttgatcgg gttaactatt ttactactta caaagcggta gaataactgc gcatcagtaa
    40801 taaatgacac acagcaaaat gaatccgttt atttgggtac ttataatcct gatgacgcta
    40861 gacgcgctgc gggaattggc tggcgcttcg tctattttag gatggctatt aacgttggtt
    40921 tgagcaagca ataagtccgg acgtgtagtt accgcagtcc gggcttattt ttcaggcgtg
    40981 cagacgacga tgcaaacgcg tcccgaccag cagagcaatg accagcatca gagcaataaa
    41041 tgcgccgacg ccgttccagc catagttatg ccagaaaaca ccacccagcg tcccggcaat
    41101 actcgacccc agatagtaac tgaacagata cagcgaggag gcctggcctt tagcgcgttt
    41161 tgcgcggggg ccaatccagc tgctggcaac tgagtgggct gcgaagaatc ctgctgagaa
    41221 gagtaacatt ccggcaaaga tcagccacag cgagctgaat aaggtcatca gtaaaccaaa
    41281 cagcataacc cccgtcgaaa acaacatcac cggaccgcgc ccgtagcggg tggtcatggt
    41341 tccggctttg ggtgagctcc atgtaccggt caaataagcc agcgataata agccaaccac
    41401 ggcctgactg acatgccagg gggagagcat caaccgatat ccgatgtaat taaacagcgt
    41461 gacgaacgac cccatcagca aaaagccttc tgcgaacaat aacggtaatc cccggtcacg
    41521 ccagtgcaga cgaaagttga taaacaacgt cttaggtcgc agcgaagtcg ggcgaaaatg
    41581 gcgtgattca gggaggattt tccagaacat caacgccgag gctagcgcga aacaaccgat
    41641 tgccgccaga gcaattcgcc agttgaaaaa gtccgtgaag acaccactaa ttaagcgccc
    41701 gctcatgccg ccaattgagt tgccgctgat atacaacccc atcgaaaagg ccacgaaact
    41761 ggggtggatt tcctcgctaa gataagtcat gccaacagct gccacgccac ttaacgaaag
    41821 cccaatcaag gcgcgcataa tcaaaatgcc gtgccagctg gtcatcattg tcgaaagtaa
    41881 cgtacaaatg gaggccaaca gtagcgccgt gaccatcact ggtttgcgac caatggcatc
    41941 ggataggggg ccagtaaaca gcaaaccaat agccaacatc gccgtggaaa tggacagtga
    42001 aatactactg ttcgcgggcg ttaagccaaa ctcctgcgaa agcaccggaa ggataggctg
    42061 cacacaatag agaagtgcaa atgttgccag tccggcagag aacagcgcca gggtgacgcg
    42121 cataaattgc ggcgtaccgc gtttaataaa ttgatttggc tgagaaatgc tttgcttgtc
    42181 agtgtcgctt gccggagcgc catcaacagt tgtagtacgg ctcacttgaa atccttgcta
    42241 aatatgcctg tggatcaggc ttatacatag ggtaggaaaa tcgaattgtt ctgtctaata
    42301 tattaataat ctcaaataag atgttttaaa tatgaatatt gaacttcgtc atctgcgtta
    42361 ctttgttgct gttgcggaag agctgcattt tgggcgcgcg gctgcccgcc tgaatatttc
    42421 gcaaccgccg ctaagtcagc agattcaggc actggagcaa caaatcggtg cccgactgct
    42481 ggcacgaact aatcgcagtg tattgctgac ggcagcagga aaacagtttc ttgcagatag
    42541 tcggcaaatc ctgtctatgg tggatgacgc tgccgctcgc gctgaaagac tgcatcaggg
    42601 tgaagcgggg gagttgcgta ttggttttac ttcgtccgct ccttttattc gggcggtgtc
    42661 tgatacgtta tcgctgtttc gccgtgatta tcccgatgtc catttacaaa cccgcgaaat
    42721 gaacactcgt gagcaaatcg ctccgctcat tgaaggaacg ctggatatgg gattgctgcg
    42781 taacacagcg ttaccggaga cgcttgaaca cgcagtcatc gtccatgaac cgcttatggc
    42841 gatgatcccg cacgatcatc ccctggcaaa taacccgaat gtaacgctgg ctgaactggc
    42901 gaaagaaccc ttcgtctttt ttgatccgca cgtcgggaca gggctgtatg acgatattct
    42961 cgggctgatg cgacgttacc atttgacgcc cgtcatcact caggaggtgg gcgaggcaat
    43021 gaccatcatc ggtctggttt ccgccggtct gggtgtttca attttgcctg cgtcatttaa
    43081 acgtgttcag ctcaacgaaa tgcgctgggt gccgattgca gaagaggatg cggtttctga
    43141 aatgtggttg gtctggccga aacatcatga acaaagtcca gctgcgcgta actttcgtat
    43201 tcatctgctg aatgctctca ggtgagggaa atttcagcga aaaagcccga aaaatgtgct
    43261 gttaatcaca tgcctaagta aaaatttgac gacacgtatt gaagtgcttc accatagcct
    43321 acagattatt tcggagcgcg aaaatatagg gagtatgcgg tggttgctga aaaccagcct
    43381 gggcacattg atcaaataaa gcagaccaac gcgggcgcgg tttatcgcct gattgatcag
    43441 cttggtccag tctcgcgtat cgatctttcc cgtctggcgc aactggctcc tgccagtatc
    43501 actaaaattg tccgtgagat gctcgaagca cacctggtgc aagagctgga aatcaaagaa
    43561 gcggggaacc gtggccgtcc ggcggtgggg ctggtggttg aaactgaagc ctggcactat
    43621 ctttctctgc gcattagtcg cggggagatt ttccttgctc tgcgcgatct gagcagcaaa
    43681 ctggtggtgg aagagtcgca ggaactggcg ttaaaagatg actcgccatt gctggatcgt
    43741 atcatttccc atatcgatca gttttttatc cgccaccaga aaaaacttga gcgtctaact
    43801 tcgattgcca taaccctgcc gggaattatt gatacggaaa atggcattgt acatcgcatg
    43861 ccgttctacg aggatgtaaa agagatgccg ctcggcgagg ctctggagca gcataccggc
    43921 gttccggttt atattcagca tgatatcagc gcatggacga tggcagaggc cttgtttggt
    43981 gcctcacgcg gggcgcgcga tgtgattcag gtggttatcg atcacaacgt gggggcgggc
    44041 gtcattaccg atggtcatct gctacacgca ggcagcagta gcctcgtgga aataggccac
    44101 acacaggtcg acccgtatgg gaaacgttgt tattgcggga atcacggctg cctcgaaacc
    44161 atcgccagtg tggacagtat acttgagctg gcacagctgc gtctcaatca atccatgagc
    44221 tcgatgttac atgggcaacc gttaaccgtg gactcattgt gtcaggcggc attgcgcggc
    44281 gatctgctgg caaaagacat cattaccggg gtgggcgcgc atgtggggcg cattcttgcc
    44341 atcatggtga atttatttaa cccacaaaaa atactgattg gctcaccgtt aagtaaagcg
    44401 gcagatatcc tcttcccggt catctcagac agcatccgtc agcaggccct tcctgcgtat
    44461 agtcagcaca ttagcgttga gagtactcag ttttctaacc agggcacgat ggcaggcgct
    44521 gcgctggtaa aagacgcgat gtataacggt tctttgttga ttcgtctgtt gcagggttaa
    44581 cattttttaa ctgttctacc aaaatttgcg ctatctcaat ttgggccagg aaagcataac
    44641 ttagactttc aaggttaatt attttcctgg tttatatttg tgaagcataa cggtggagtt
    44701 agtgatgctg aagcgtttct ttattaccgg tacagacact tctgtaggga aaacggtggt
    44761 ttcccgcgca ttgctacaag cgttagcctc ccagggaaaa acggttgcgg gatataaacc
    44821 cgtagcgaag gggagcaaag agacacccga agggctgcgt aataaagatg ccctggtgtt
    44881 gcagagtgtt tcaaccatcg aactgcctta tgaagcagtt aatcctatcg cgttaagcga
    44941 agaagaaagt agcgtggcgc acagttgccc aatcaattac accctcattt caaacggcct
    45001 ggcaaacctg accgaaaaag tcgatcatgt cgtggtagaa gggactggcg gctggcgcag
    45061 tctgatgaat gatttgcgtc cactctctga atgggtggtg caggaacaac tgccggtgtt
    45121 gatggttgtc ggtattcagg aaggttgcat taaccatgca ctgctaacag ctcaggcgat
    45181 tgccaacgac gggctgccgc tcattggctg ggtggctaac cgaatcaacc caggactggc
    45241 gcattatgcg gaaatcattg atgtgctcgg taaaaaactt ccggcaccgc tcattggtga
    45301 actgccttat ctgccgcgcg ccgaacagcg tgaactgggg caatacatcc gcttagctat
    45361 gctgcgcagt gtgctggcgg tagatagagt cacggtgtaa cgtccgcgag attaccgacg
    45421 caattacgca ggcaatcaat aaaccgggga gtagctgata ctccccggtc atttcacata
    45481 tcatcaacgt cgacataatc ggcgcgtgcg tggttgccgc caacagtgtc gccatcccgg
    45541 tcaatccgag taaaagcgta atttcttcgc catcagggaa ccataatccc aggctacgac
    45601 catacaacat gccaatggca agaccgataa atagcgtcgg tgtaaagaca ccaccgggtg
    45661 cgccggaacc gctactcgcc agcacggcac acagtttaca aaggaagatc ccggcaatga
    45721 tcattaacag tggcggggcg gttaaaaagg attgtacggt gctatagccg ttgccccaca
    45781 ctgcaggtgt gaacagggaa agcagaccca cgatcaaccc gcctagtgcc agttgccagg
    45841 gcggcgcaag tttgagaccc acaaatccac gatgacaggc gttcattaac gttaacaaca
    45901 gtggtccgca cagacctgcc agcacacctg tactgataat cagcgcatag tcacgagcct
    45961 gaaccgtcac tgagagttgt acgttgtaga gtaacgcgtc gctatgatta atcagattgc
    46021 taaccagcaa tgccacgacg gcggaaataa tcaccgggcc gagagaggcc aacatcatag
    46081 tgccaaacag cacttcggct ataaataaac tgccagcaag cggggcacga taggccgcag
    46141 ccattcccgc cgcggcccca caggcgatcc ataatttcca ctcctggcgt ggcgtaaaac
    46201 gttgggcaaa acaggaggcg gcaagggcag ctaaaagaat catcgcacct tcgcgaccaa
    46261 ttgcactgcc gctggttact accagcagcg aggcaagcga tttaaccagg cttgctgcgt
    46321 aatcgaactg tccatcggtt tgcaacgctt ccatgtaatc ggtcggcgca tgagggcgtt
    46381 gttgggtaaa tttctgccag cccatcagca acaaacccgc cgccagtccg ccgagcgccg
    46441 gagttagcag ccgtcgccag ggggaaaggt ttgtcgctgc attgaccaga ctgccggagt
    46501 cattattgag gaacaaccac tccagtagca gcatcgcatg atgaaacccg gcaacggcaa
    46561 aggccgcgag aataccgacg actgttgcga taagcagacg gcggaacata gtgcgcagat
    46621 ccggatagat gtgtagatga tgcatgagca gaaatgggca atgaaacacg ggcgactatt
    46681 ttgcgacaaa agaggccagc cgcaaaagca gtgcgtgctt taagagataa aaaaacgtga
    46741 cactgtaacc tattattgcc ggattgcgcg taatcgtcac catccggcaa tattacggtg
    46801 atcctatcga aacagcggtt taaccgcgac aggaatcaac agttgtgatt gccattgcgc
    46861 cagcgttaag cgagccagtt cacccagtgc acggtagaaa gggtgttctg ctttttcgat
    46921 aaaaacatca agaaaacgtg ttgaccacgg aaaaaggtgc catgccagca attcttcgca
    46981 ttctgtctgg cgaccattct ccgccaacca cgcagccatc aacagcagtg aaccaaaatg
    47041 atcttccggt tcgttttgct tcatttcaaa ctgaatgcct ttctcgcgca tccactgacg
    47101 aagtgccaat gttgaatcgc caaacagcac agattcgcga tccagccaga ccgaccccca
    47161 cggcggagac ggcagtgccc acgggccgac aaacaaacgc tgccaggcct gggcgtgagt
    47221 ctcttcacac tgtgtctgaa aagccgtcac cagcggcgct aatgacgctt ctggtagagg
    47281 ccactgagtt tcccagccgt cactggtgag taccgcaacc agaggtgcgg cttccgcact
    47341 ctctggagcg taataaaaca gcgcgcccaa cacgcgcgcc gcgacagaaa aattatcttg
    47401 ctgtgaaaaa tgggtcattc ctaacatcct gaaatgtgcg ggttgccccg caccaatttg
    47461 ttaacctgca atagccattc ctaccgtcat gtgcaggcca taaaagagca cacgcccgat
    47521 catctcaccg ccaagaatga ggattaaccc cagaataagt ccggcaacgt gaggttcacg
    47581 gcgacgaatg agcggacaaa cccagcatcc tagacctgcg cataacaata ccacacgcca
    47641 gacctgtaag gaggcgtaat ctggtaccag tgcgctggct tgttgtacgg atgagtgaat
    47701 acacgccaga gacaaacctt gcagcacaat caccccggca caagcgatca gcgccagaac
    47761 gctaatgatg gcaaatggtg tggtattaaa tgtaacgcga gctgcccgca gaattgcggc
    47821 agcaagtatt ggaccgctca acaataccgt caggaagaaa gccagcgtag tgtaaccgtt
    47881 atgccaggtt ggcacggtgt cgatttgata cacgcaggtc atcatccaga cgaaaatgac
    47941 gccgagcgcc atgctgacaa gtaaccagag tttccccaac gcttgcggca ttttaccgat
    48001 gaccgccacc agccaccata gtccgccaac ggcaaaaaat atggaaccgg cagcaatctc
    48061 gttactcaga ccagaagctc cgattcgatt cagcgagtta aacgctcgca gtggcgatcc
    48121 caggtgcatg acagaggcaa taaacccgat gcccatcaac agccagagga aaaacatgcc
    48181 acggacaata cgctgacggt ctgcgtcatt tttggcggcg aaccagccaa ttccgctaac
    48241 aatcagcgcg cctactacgc attgacccaa tacggtaaag ataaccagtg gccattcatg
    48301 ccatccattt cccattttac acttcctccg gattagccag ataaccagtg gtatcgccgg
    48361 tcgggcggct gttggcgtta ggtttgataa cgatattggg ttttgtgaaa tgcgcgcgcg
    48421 gcagtggcgc gacggcagcc agagtgccgt gcttctggcg cagctcttca attggaccga
    48481 actcaagtgc acgcagcggg caggattcga cgcatatggg ttgtttcccc tcggcgacgc
    48541 gcgaataaca accatcgcac ttcgtcatgt gccctttttc agcattgtac tgtggtgcgc
    48601 cgtacggaca agccatgtgg cagtagcggc agccgataca aacatcttca tcaaccacca
    48661 caaagccatc ttcacgctta tgcattgcgc cgctggggca gacttttgta catgctgggt
    48721 catcgcaatg gttacaggag atggagagat aataagcgaa cacgttctgg tgccagacgc
    48781 cattatcttc ctgccagtcg ccgccagcat attcataaat acggcggaaa ctgacttccg
    48841 ggccaagatc tttaaaatct ttgcacgcca gttcgcaggt tttacagcca gtacagcggc
    48901 tggaatcgat aaaaaatcca tattgtgtgg tcatgggcta ctctttaaac cttttcgatc
    48961 tggacaagat tgctgtgcga cgggtttccc tttgccagcg gtgaagggcg gtgagaggtc
    49021 agaatattga tactgccgcc gtgatcgacc cggtcaccaa acatatccgc tttaagccac
    49081 gcaccttgcc cgatggcggt aacgccaggc agaatacgcg gagtcacttt tgcggcaatc
    49141 agcatttctc cattattgtt aaatacccgc acggtatcgc catgacggat accgcgagcc
    49201 tgagcatcaa tggggttgat ccacacctct tgtgggcagg cctgctgtaa cacatcaata
    49261 ttgccgtagc tggagtgggt acgcgccttg tagtggaagc ccgttaactg cagtggatag
    49321 gttttccgca ggggatcgtc ccagccatca aaacctggcg tataagctgg aaggggatga
    49381 ataatttcat cttttttcaa ttcccaggta tccgcaatct tcgccagtcg ttcagaataa
    49441 atttcgattt tccccgaagg tgttttcaac gggtttgcct ggggagcttc acggaatgcg
    49501 cggaaagcga cgtagtgttc ttccgggcat tttttcttaa agatcccggt cgttttcatc
    49561 tcctcgtagt cgggcatctc agggttacgt tccttcgttt tcgcatggag atatttgatc
    49621 cattcatgct gactgcgacc ttcagtaaag gtttgataaa cgtctggtcc taagcgtttg
    49681 gcgacttcac tcagcatcca gtagatgggt ttgcgttcaa attttgcaga ggttgcgggt
    49741 tgggcgagga tcacatagcc catattccct gcagattcat gagagataag gtcttcttgc
    49801 tctgttggca tcaggtcggg caacaggata tcgcaatact tagccgaggc cgtcatgaag
    49861 tggtcaatgc caacaatcat ctcgcacttg ctgtcatcct gaagcacctc atgggtgtga
    49921 ttgatgtcgc catgttgatt gatcaatgtg ttactggcgt agcaccataa aaacttgatg
    49981 gggacatcca gtttttcttt tccacgaaca ccatcacggg tcgcggtcat ttccttacca
    50041 tggtcgatgg catctgtcca tgtaaagacg gaaatctgcg ttttaacagg gttctcaagc
    50101 atcgggaacc attctacccc cagatcccag ctaccttcgc gtacacctga gttgccgccg
    50161 tttatgccga cgttaccggt gagaacggaa agcatggcaa tagcgcggga cgtttgctcg
    50221 ccgttggaat gtcgttgtgg cccccaaccc tgacaaatat aagcaggttt tgctgaaccg
    50281 atctctcgtg ccaactggat aattttttct gccgggatgc tggtgatttt tgctgcccat
    50341 tccggcgttt tagctatgcc gtcaggccct tcgcccagaa tataggcttt ataatgcgcg
    50401 ttacgtggtg cgttggcggg cagagttttt tcatcgtaac caacacaata tttgtcgaga
    50461 aatggctgaa cgaccatgtt ttcagtaatc agtacccagg caatcgcaca ggccagtgcg
    50521 ccatcggtgc ctgggcgaat gggcagccat tcatcttcac gcccggcagc tgtgtcgtta
    50581 taacgtggat cgatgacgat catgcgtgcg tttgaacgtt cgcgggcttg ctcgacgtag
    50641 taagtgacac caccgccgct catccgcgtt tctgccgggt tatttccgaa cataacgacc
    50701 agtttcgtat tggcgatatc atccgggctg ttgccatcat tggcaccgaa catataactc
    50761 attgcggcac tgatctgtgc ggtactgtag ctgccatagc gactgagaaa accaccgcaa
    50821 gagttcatca gacggtacgg gacgtttgag ttggtgatgt ttccgccatc tacgcctgtt
    50881 ccgtacagga catgtacagc ctcattgccg taatctttta ggatccgccg aagattatca
    50941 ctgatggtat ccagggcttc gtcccaactt atccgttcaa atttaccttc accgcgcttg
    51001 ccgacgcgct tcatgggata tttcaaccta tcaggatgat tcatccgtcg gcggatagag
    51061 cgcccgcgta aacacgcgcg aacctgatga ttaccgtaga cgtcgtcacc tgtcgtatca
    51121 gactccaccc agtacacggt gtcatctttc acatgcaaac gtaacagaca gcggctcccg
    51181 cagttaacgg tgcaggaact ccagaccgct ttctcttcta ccggagcctc tgccgcccgg
    51241 accatttgag aaaatggcag agtgaaagca ctgcttgcca gcgcaagact gccaagtgcg
    51301 gaggttttca tcagacttct acggctgatt tcagccttca tgagcgcctc tgtggtatgg
    51361 attttcatca ttactcactt attgcttttc aaacaaaatg tcatgccaga atttatggtt
    51421 gtcgtgggtt atattttttc gatctcgacc agattagtgt gctgcgggtt tcccttcgcc
    51481 agtggtgaag ggcgcagagt ggttagcgta ttcacacagc cgccatggtc gattttatcg
    51541 ccagacatat tggcctcgtg ccaggctccc tggcccatag cgctaacccc agggagaatg
    51601 cgtggtgtca ctttggctgg tagccgaact tcgccacgat ggttaaacac ccgcaccata
    51661 tcgccgttgg caatcccacg tttctgtgca tctatagggt tgatccacac ttcctgacgg
    51721 caggctgcct tcaggacatc aatgttgccg taggtcgagt gcgtacggga tttgtaatgg
    51781 aaaccaaaca gttgcagtgg gaaggtactc cgttcagggg agtcccagcc ttcaaaggta
    51841 gaggcataaa ctggcagtgg gcttatcact tcatcttttt ccagttccca ggtacgggca
    51901 atttccgcca gcttgctgga ataaatttca atcttaccgg aaggtgtttt aagtggattt
    51961 gcctcggggt cgtcacgaaa tgctttgtag gcgacaaaat gaccattggg atctttacgc
    52021 ttatagatac ccattttttt cagttcgtcg taagacggta acgccggatc tttggcaagc
    52081 attttggcgt acagatgttg taaccattgt tcctgcgtgc gaccttcagt gaacttttga
    52141 tagacgtcag gtccaagacg tttcgcgact tcactcagga tccagtaaat cggtttacgt
    52201 tcgaattttt cgctggtgac tggctggagg aaaatgagat atcccatgtt accggcgtag
    52261 tcgttaggaa taatatcttc ctgctcaacg gtcatcaggt ctggcagcag aatgtcggca
    52321 tattttgccg atgaggtcat aaagttttcg atgaccacaa tcatttcgca tttcgattcg
    52381 tcttgcagaa tttcatgcgt tttgttgatg tcagaatgct gattaacgag ggtatttccc
    52441 gcgtagttcc agatgaactt aatgggcaca tccagtttat ctttgccgcg gacgccgtcg
    52501 cggattgccg tcatttgcgg accatgatcg atagcatctg tccagctgaa gcaggagatt
    52561 gacgttttga ccggattatc cagcaccggc aggcgttcta tggtaatggt ataggtcgat
    52621 tcacgcgcgc cactatttcc gccgctgatg ccgacattgc ccgtcaaaat aggtaacata
    52681 gcaatagcgc gtgcagtcag ttcgccgttt gcctggcgtt gcggccccca gccctggcag
    52741 atataagcgg gttttgctgt gccaatttca cgcgccagtt tgatgatacg gtctaccggg
    52801 ataccggtaa tttgcgaagc ccactgcggc gttttcgctg tgttatcatc accttcacca
    52861 agaatatagg ctttatagtg accatttttg ggtgcatctg cgggtaaggt tttttcgtca
    52921 tagccgacgc agtatttatc gagaaaaggt tgatcaacga gattttcgtt aatcaatacc
    52981 caggcaatac ccgcaaccag cgcggcatcg gtgcccgggc gaatagggag ccattcatct
    53041 tcacgaccag cagccgtatc ggtatatcgc ggatcgataa caatcatttt ggcgttcgat
    53101 ttctcgcgcg ctttttcaag aagataagtg atgccaccgc cgctcatgcg ggtttctgcc
    53161 gggttgttac caaacatcac gaccagcttg ctgttttcaa tatccgtggt gctgttgcca
    53221 tcattactgc cgtaggtgta gggcatggca caggaaattt gcgcggtgct gtaggagcca
    53281 tactgattga gtgaaccgcc gtagcagttc atcaggcgtt tgaccgccga ggctgatggc
    53341 gaagagcggg tcatattgcc gccaacaatc cccgaagagt actgaatata tacagcctca
    53401 ttgccatatt gttcgacggt ttttttcagg ctactggcga tagtatccag ggcttcatcc
    53461 cagctaatcc gttcgaattt gccttcaccg cgtgtaccca cgcgtttcat tgggtaattc
    53521 aagcgatcgg gatgattaat acgccggcgg atggagcgac cgcgcaaaca ggcgcgtacc
    53581 tgatggttgc cgtactcatc gctgccggta ttgtcagttt ccacccaggt cacttcatta
    53641 tctttaacat gtagacgaag tgcacagcgg ctaccacagt tgacggaaca ggcaccccag
    53701 atcacttttt cgctggcctg ttgtaccgtt gccgctgcac tgcgcagggt gaacggtaaa
    53761 gaaaaaccgc ctgcagccag cgccagagaa cctatcgcgg ttgatttaac gagtgttctg
    53821 cggctgatgc ccaccattcg gtcatttttg gacataactc actccctgtt ctttatcgtt
    53881 atataaatgt ttatatattg aatatttagc gcgctaacaa tagagggagt ctacccattt
    53941 tgggttaaga attattaatc catatcaata gaagggtatg agtaataagg tgggattatg
    54001 ttgtatgttc aaatcgccgg atgtgtcgta tccggcgttc agtcgataat gtattactgc
    54061 ggttcggcag gcgcgccatc ctgggtagac tgcgcgggag cagagacgtt accgctggtg
    54121 gtgcgggtat agagaatttt atgcgtatca ttagcgcaat ggccgacgac ctgggaatca
    54181 ggctgatcaa cctggtcatt gggtacaata cttaacgtga agctgctttc gggtacgcca
    54241 ttattgataa tgcgctgtga tatatcgctc tgtatgcgct cacaggatcc cggcgcggcg
    54301 agtaccacgg gtgaggcgag ggcgagcaga agcgcggcac agcaggttga gagtttcatc
    54361 ataagctcct tacgcgaaga taacttcttt aagcatagca tttaacgtgt aaagtactgt
    54421 atttgctact atgatttaga atcatctcta ctctctggtg actgttgtga aatacaaatt
    54481 actaccatgc ttactcgcga tattcctcac aggatgtgac cgcacagagg taacactttc
    54541 atttacccct gagatggcca gtttctctaa tgaattcgat tttgatccgc tgcgtggtcc
    54601 ggtaaaagat ttcactcaga cattaatgga tgagcaaggt gaagtgacga aacgtgtttc
    54661 tgggactttg tcggaagaag gctgttttga ttcactcgaa ttactggatc tggaaaataa
    54721 taccgtggtc gctctggtac tggacgccaa ttattaccgt gatgccgaga cgctggagaa
    54781 gagagtacgt ttacagggaa aatgccagct agcagaatta ccttctgccg gggtgagttg
    54841 ggaaaccgat gataatggct tcgtgattaa agccagcagc aaacaaatgc agatggaata
    54901 tcgctatgat gatcagggtt atccgctggg taaaaccacg aaaagtaacg acaaaacatt
    54961 atctgtcagc gccacgccat caacggatcc gatcaaaaaa ttagattaca cagcggttac
    55021 tttactgaat aatcaacggg ttggtaatgt aaaacagagc tgtgaatatg acagtcacgc
    55081 taatccggtg gactgtcagc taatcattgt tgatgaagga gtaaaacccg ccgtcgaacg
    55141 ggtttacacc atcaaaaata cgatcgatta ttattaatgc tattgtgcgg tcggcttcag
    55201 gagagtctga cccggtgttt tgtgctctgc cagatactga tgctggaata tacacatgcg
    55261 aatggcatta cgatattgac cattaataaa gaactcgtgc atcaattcac cttcaaccga
    55321 aaagccaagc ttgcggtaaa tgtgaatcgc tttttcattc tctttatcaa cgatcagata
    55381 cagcttatag agattgagaa cggtaaagcc atagtccatt gctaatttgg cggcacgggt
    55441 tgccagacct ttcccctgat actccgggga gataattatc tgaaattctg cgcggcgatg
    55501 aacatggtta atttccacca gctccaccag accggctttt tcgccgtcac attccaccac
    55561 aaagcgccgt tcgctctgat cgtgaatatg cttatcatac agatcagaga gttcaacaaa
    55621 ggcttcgtag ggttcctcaa accagtaacg catcacactg gcgttgttgt cgagttgatg
    55681 tacatagcgt aaatcttcac gctccagcgg gcgtagctta acactgtggg cgcttggcat
    55741 aacgtgtcct tacattcctt aaatcaataa caggttaggg ggtaataacg cggccagttc
    55801 gacggtccag gcagcgcaaa gtattgggct cccagtaggc attgatgttg gcgctttgct
    55861 cacatttatc gcggttatca aaagcggcgt cggctttatc ccactctttt tcagtgcgtt
    55921 tattcacttt ctggcgcaga ttgcgcgtgt cattccattg ctctttttcc atagcggcgt
    55981 gctggcggct ttgtgcactg tcgccagact caatcaccag tttgttagtt tcggcatgaa
    56041 cagttgtgct caatgccagt gcgcaaggca gcagaatagc gagcaggccg attcgtttgc
    56101 tgagagtgat tttcataatt cattccctgt atgaatgatt aaaggtgatt ctacaccatc
    56161 cactgcggac gcaaaacgta ccaggagggt gtttatattg atgatattat gtcgccctat
    56221 aactatacat gatgtcaata agagacaaag atgattaaaa caacgttact attttttgct
    56281 actgcgctgt gtgaaattat tggatgcttt ctgccctggt tgtggttaaa acgaaacgcc
    56341 agtatctggc tgttgcttcc ggcggggatt tcactggcgc tgtttgtctg gttgttaacg
    56401 ttgcatccag cggcgagtgg gcgtgtttac gcggcttatg gtggcgttta tgtctgcacg
    56461 gcgttgatgt ggctgcgcgt tgtggatggc gtgaaactga ctctttatga ctggacgggt
    56521 gcgttgattg cgctttgcgg catgttgatc attgttgcgg gctgggggcg cacgtaggaa
    56581 cataaatcca ttttatcaat aagataagag gaagtgtcag ctgacaaaag gtattctatt
    56641 tcatcttttg tcaaccattc acagcgcaaa tatacgcctt tttttgtgat cactccggct
    56701 tttttcgatc ttcatacttg tatggtagta gctcagttgc gtagatttca tgcatcacga
    56761 caagcgatgc aaggaatcga acatgaagat cgtaaaggct gaagtttttg ttacctgtcc
    56821 ggggcgtaat ttcgtcacat taaaaatcac cactgaggac ggtattacgg gccttgggga
    56881 tgccaccctc aatggacgtg agctttccgt ggcctcttat ttgcaggatc acctttgtcc
    56941 gcagcttatt ggtcgcgatg cgcaccgtat cgaagatatc tggcagtttt tctataaagg
    57001 tgcttactgg cgtcgcggtc cggttacgat gtcggccatt tcagcggttg atatggcgct
    57061 gtgggatatt aaagccaaag ctgccaacat gccgctttac cagttactcg gcggcgcgtc
    57121 tcgtgaaggg gtgatggttt attgccatac caccggtcac agtattgatg aagctctgga
    57181 tgattatgcc cgtcatcagg agctgggatt caaagccatc cgcgtgcagt gcggaatccc
    57241 tggtatgaaa accacctacg gcatgtcgaa aggtaaaggt ctggcttatg aacccgcaac
    57301 caaaggacag tggccggaag agcagctgtg gtcgacggag aaatacctcg atttcatgcc
    57361 gaaattgttt gacgcggtac gtaacaagtt tggttttaat gaacatttgc tgcatgacat
    57421 gcaccatcgc ttaacgccta ttgaagcggc gcgctttggt aaaagcattg aagattatcg
    57481 catgttctgg atggaagacc cgacgcctgc ggaaaaccag gaatgcttcc gtctcattcg
    57541 ccaacatacc gtcacaccca tcgcagtggg tgaagtcttc aacagcatct gggactgcaa
    57601 acaactgatt gaagagcaac tcatcgatta tatccgcacc acgctgaccc atgcaggcgg
    57661 aattaccggt atgcgccgga ttgccgattt tgcttcgctg tatcaggtac gtactggctc
    57721 acacggtcct tccgatttgt caccagtctg catggctgcg gcgctgcact ttgatctgtg
    57781 ggtccccaat ttcggtgtcc aggagtacat gggttattcc gaacaaatgc tcgaagtctt
    57841 cccgcacaac tggactttcg ataacggcta tatgcatccg ggagacaaac cgggtcttgg
    57901 catcgaattc gatgaaaagc tggcggcgaa atatccctat gaacctgctt atctgccagt
    57961 cgcacgtctg gaagatggca cgctgtggaa ctggtaagga gtaaggtaat gaaaagcatt
    58021 ttaattgaaa aaccgaatca actgtcgatt atcgaacgtg aaatacccac cccgtcagcg
    58081 ggtgaagtac gagtaaaagt gaaacttgcc ggaatttgtg gttcagatag ccatatttac
    58141 cgtgggcata atccttttgc gaaatatccg cgcgtcattg gtcatgaatt ctttggcgtc
    58201 attgatgcgg tgggtgaagg tgtggaaagc gccagagtcg gtgaacgcgt tgctgtcgat
    58261 ccggtggtca gctgtgggca ttgctatccg tgctctatag gtaagccgaa cgtttgtacg
    58321 acacttgctg tattaggtgt gcacgctgac ggtggtttca gtgaatatgc cgtggttccg
    58381 gcaaaaaatg cgtggaaaat tcctgaagca gtggccgatc aatatgcggt gatgatcgaa
    58441 ccttttacca ttgcggctaa cgttaccggt catggtcaac cgactgaaaa tgataccgtt
    58501 ctggtttatg gtgccggtcc aatcggcctg acgatcgttc aggtattaaa aggcgtctat
    58561 aacgttaaaa atgtgattgt tgccgatcgc attgatgaac gactggaaaa agcgaaagag
    58621 agcggggcag actgggcgat taataacagc cagacaccgc ttggcgagat tttcgctgaa
    58681 aaaggcatca agccgacatt aattatcgat gcggcttgtc atccttctat cctgaaagag
    58741 gccgtaacgc tggcttctcc agcggcacgt attgtattga tgggcttctc cagtgaaccg
    58801 tctgaagtga ttcagcaagg aattaccgga aaagaactct ctattttctc ttcacgctta
    58861 aatgcaaata aatttccggt tgttatcgac tggttaagta aagggttaat taaaccagaa
    58921 aaattaatta cccatacgtt tgatttccag catgttgctg atgccattag tttatttgaa
    58981 caggatcaaa aacattgctg caaagtctta ctcacttttt ctgaataata ccaataacgg
    59041 cgagtaagta gtacgcatct tacctctttt ttagagataa ccattatgac aatagaaaaa
    59101 cacgaaagaa gcactaagga tttggtgaaa gcagcagtat cgggatggct gggcactgcg
    59161 cttgaattta tggatttcaa gagtcatgcg tgttaactat ttgataaata ttaaattaat
    59221 ttttcattgc ttcgttatgg ggcatggttg gggcaaactc gcttaactgt gtatttaaca
    59281 aagctacctg tgcattattg ttttcagaca tccattttcc gtatacctga aataccattt
    59341 gcgcatctgc atggcccatc tggtttgcta taaatgccgg gttagcacca gctgtcagcg
    59401 accagcaggc ataagtatgt ctcgactgat atgattttcg atggcggagt ccggcacgtt
    59461 ttatcgctgc gtcccacatc tgccttattg agtcaacggt aaaatggtca ccataatttt
    59521 ttactctcgc tgacacttca ggttgaaaaa caaaggtgca tttttgtttt tctgttctgc
    59581 catactctct gaggtgaaca tcaatgatat gctctttgct cagtctcgtt aatgtcatct
    59641 gactccggag agcgtcgatt gctggcttaa taagatgaat gacccgattg gttcccgcct
    59701 gtgtttttgg taccgtgaaa cggtcttttg ctaaatttct cctgatcatc attgttccat
    59761 ttttcagatc tatgtcctcc catccaagtg cacacagctc accagggcga actccagtat
    59821 aaacagaaac acaccataaa ttttttgctt gctgatttct gcacgcatcg ataagacgga
    59881 taaattcttc ccgcgaaaga ggatccggaa tggttcttga ttcctttaat ggcgagatcc
    59941 ccttaaacgg attatctgcc aggtaaccgt tatcaacacc aaactggaac acggcgttaa
    60001 gatttgtcat gtaattattt acagttacag ccgatctccc tggttgtgta acaatatagt
    60061 tacttttggg gatctggtat ccagtcagta actctttacg aacctccagt aatttttctt
    60121 tattaatcga tgaggcaaga tttttttcac cgattatgct caggatattt ttgatgacgg
    60181 cacggtatgt gttgagtgat gttttggcga cttcagtttc tttcagtgcc agaaattttt
    60241 cagccagttc ttttatggtt aaatcttgtc gggcctcacc aaatttttcc agattgcgtg
    60301 aggagggaaa ctgttttgca tagtcgaaaa caccagtttt tattgcgtaa caaacagagg
    60361 agcgtagttc acctgcaacg cgcctgtttt ttgctgtgtc aggaaccccc agattttccc
    60421 tgactcttac gtctttataa acaaaccaga tacgtaattt ccctccatgg ttttccacgc
    60481 ctgtcggata tttcatttca acttctctca ttagttagtg tggcttttag tcaagtaaga
    60541 tgacgtcttg gtctcgctga tgcctggcgc tcaatccagc gatcaatttc ttccaggttg
    60601 taaaagcatg gactgttatc ccatggcata ccgtcatgag cgacatgctt atattccctt
    60661 ccttccataa acgatttttc ccgggccttt tttaacgtac ctttttttat tcctttcagc
    60721 gcaattaact gctcttcgga tacccatttg ccgggagaga caatcatgat tacttcgctc
    60781 atcgatttct ttatctctta catcagacga gcgccggttg cagaatacca gtcacaaccg
    60841 gcgacagttg aacattaaga atcagcctga ctcgggatca gtttttgcca gataactgaa
    60901 acgtattttg cctggtaacg ggcgtcatca agtgcattat ggcgctcacc ttcgaatgga
    60961 atagccgttc tggcatcgaa gtctatggct ttccccagct caacgattgt gcgtacatcg
    61021 cgatcgttgt agtaacgcca cgggcagggg atcccctgcc gttcgtatga acggcgcaaa
    61081 atcgtgttgt cgaagttggc tccatttccc caaacctgaa caaaaaattc accggagttt
    61141 tcgtcgataa attctcgcaa ttgtaacagt gcatcatcta acgggatttc atcggtcata
    61201 atggcagatt gcgcttcgcg tgattgctta agccaccatt taatggtgtc ccgatcaatg
    61261 actccgccag cagtttccag atcgatagtc ttactaaatt ccggtcccat atctccggtt
    61321 tgcggatcga aaaatattgc acctattgag atgatcgggg catcaggatt ttttcccatg
    61381 gtttcaaggt cgatcattag atggtcacac gtcctgctgg tggatgtgat ttcgtgatga
    61441 ccgttcacct taattgggtg atctgccgtc tcgccagttt cattatcgct attgtgatgc
    61501 tgattgccgc cagtgttctc cttgtgtgga tgttcagcgc cttccatttc ctccggatca
    61561 tcttcctgaa cttcaacctg atactcttca tcgaatgttt cttggtatgt tgcgtcgccc
    61621 atcaccgcgc cacaatcagg gcagttgccg ccgccggtct gaccgcaggc ggtgcagact
    61681 ttttccactt cctgttgcgc tactggttca ggctgtttcg tttctggctc gttttgtaac
    61741 gcatttggac tgttttgttc cgctttttgg tagttccgtt ccgattcatg ctggttctgg
    61801 ttcacagaat cgcgggtctg gagcccctta acccatttcg gatcattcgg gtcactaatc
    61861 ccttcaacaa attcaccacg tgatgcagca agcaacttat cggcgtcagg ctggctgata
    61921 ttggctgcct gcataatttt gtttacttcg tcagcggtaa cttttatcgg ctctggttgt
    61981 tctgaatctt cagcggtatc tacattttgc ggtaagcccg tgtatgtgcc attttttcgg
    62041 gcaaaatatt cttcttttgt gatttcagtg gcgccagcag ccagtgcctt atccagacca
    62101 gagagtttgt ttgcgcgacc gtatttttct ccgtccttat ctgcgaagag gaaatagaac
    62161 ggcccctcac gctctacaga tggttcagct tccggcgcgg tttcattttt tgggatatca
    62221 gatacctcag tttccactgc atcagtttgt gtttctgatg actggagaac atcaacagtg
    62281 cccaggtctg tttcttcatt ctcaaacacg ccctttgtcg tcaggtattc gcagatatat
    62341 ttgttcagtg ctacgggatc tttgtgaatg tcgatcggac gctcacggac aaggccaaaa
    62401 atagtttggc ggtcgtagcg aagggcatca ggctgtttgc gcattgatgc cgagatacgc
    62461 ttccagtctt cgcggtcgtt gtcgataact tctttttttg cccagcgatg gatgctgccg
    62521 tcaatgtttc cggcatccac atcactaggc cagagagcgt aggccagttc gtcatccagt
    62581 gttttccatg tctgcttgta ttcgcgatga atggcagcaa tgaccgggct gatttttcct
    62641 gttgaatttt cagtgtactg ttgattggct ctggcgcggg cgagatcaac aacagacgtg
    62701 tattttccgg tttccttgcg ttcaccttcg cgacgttttt tccagatgcg catctctgcc
    62761 tgaatttcgg gccatttagc accaggaata catttatgct taacccaccc aatggcgtgc
    62821 aacttaagct ccggatacat ggcgttaact tctggcattt tcatcaacgc ttcaacgata
    62881 tgtccgtcga atgttgccat gtcttcctgc aacaattcct gtgcgctaat aaccatatca
    62941 acggtgatgt tttcacatgt gtcgaactta accatgacag cgttctgtac ttcaggggcc
    63001 agcttgtcaa aagtgacgtt catcggatcg gattcagtct caaccgggac aaaagaagca
    63061 gactcctcat cccagcggtt ttcctgcata tattcagcat cccatgaatc gagggcaggg
    63121 cggggtatgc caggtttatc ctcgcagaca ataaatttat aagcgcagtc ctgagcagcc
    63181 ggataatgtt ccaggaattg ccagtgaaat tttgctcgag cacggcgttc gtcgccagct
    63241 tcaatggctg tggctacagc cacagcgcct tcttcccttg ttgccagttc gtcaggaata
    63301 gcggcgcaaa taaagacttt actcatttgt tttaacctca tgacagattt aaggatgaac
    63361 aaatccctgc cattgctggc atataagaat gaaaccggat atttattacg gaactgtttt
    63421 aaagacctgc cgggatttcg atattatcct ggtgaataac tttatcgacc gggtaacagt
    63481 taccgggaat tttctgttcg gttgctgcag tcatacactc ctgcattgtc ctgtgaacac
    63541 tgactgcaat atcaactggc tctccggaaa caagaaaaac tgtcagaaca agcacaaatg
    63601 ctgaattcat tgtgcacatc cttttggcat cagacgtaaa cgagccagca ttgaaacaat
    63661 gcatatttta tttaatagct cccgttcttg ttttctcttg ttaatggcat cttcagtaaa
    63721 tactgggtta ctgatagtga caccaatttc aaaacaacct tcagacgtat taacgtttgg
    63781 taataacgtt ttcattatcg cgtcctcaac aatgaatttt gtgatgcagt gcctggtgcc
    63841 tccaggtgac gttaaccagt taacaattaa cgccggatac agagaatcca cccataacac
    63901 tgtttttggt tttaactgtt ccgcgtgcgc tcagccgcat tcaccacatc acaaaattca
    63961 ctttaaaaag ggcggcagag cagtcacgga gtaaaactga taccgccaaa cgtcaccaga
    64021 aaattgataa cagagggcgt tgcagcgggg ttgtcactta agcgtatggt caacctgaca
    64081 acccggtgtc ctcaacgggg aaggaataac cccgccatac ttaccgccgc gccatttcgc
    64141 gggttgccac aaccggaagc gcacggtcga cgaaaattta acgacaggct atctatgaac
    64201 cagctacctc gccgtgcgct ttcgcgttat ggtctgactt ttcagggaaa tatcctttca
    64261 gtaaactgtc agttccggat gcgcacccgt gtccggcgca cgcactccac ctcacccgtg
    64321 gagaactcct taattactaa ccttagcttt gttgattagc tactaacgcg ggtatgtaat
    64381 cattctggca atgcttaatg ccgctgcttt ttccagattg gtgatatcct gctccagagc
    64441 ggacagattt tcagcctgct tagccctggc ttcattagcc catttcagat cctgcgctgc
    64501 attaattttc tggcgcatcc actcataaag ttcatcatcg gtatagtctg gcgcgatgat
    64561 gacgggttct cgtttctgca tactgattcc tcgcggtgct gtttcgctta tcagccgtta
    64621 gattttgccg aactggaaag cgcctgttta aactcactga agctgagagc ttcttcgcct
    64681 tcggcaaggc cttcgaagta ttcttcgtaa gccttttcca tgattgtgtc gaaatccata
    64741 tcactcacct gagtttcttt ccagccagcg acgggcacca ttttcggttt taaacgtttt
    64801 gcttttggta tacgtcattg cggtgaacgt gccgtcctgg tttggaaaca cgccgtacac
    64861 cagagattcg ttgttgccaa gatcgatagt atccatgctg acctcatttc cccttaacgc
    64921 tggggtagcg gaactgtttg ctgagaacac cgtgcggtgt gttgatgcaa acaagattag
    64981 ccattactaa catatcggtc aagtgatttt gtatgctata gctaacataa ttgatgtggt
    65041 aaaagataac tcattgatga tgttatcttt tatttgtccg ctgacgggct tttagtaatt
    65101 cttcaaagag tttattgaag ttttttactc gagctcgcat ttcggcgagc tgggtatcct
    65161 gttctgattc tggcagtgca ttaaacagct caaggagctc tagttctttg ggggataagg
    65221 caactggctt ctcaacaggt ggtgttggtt gcttgtcttc atcgccaaat agaatccatg
    65281 ttggtgagca ttgcaatact ttgctgaggg caaaaaggtt cttccctgta ggttcactat
    65341 caccccgttc ccattgtgat acagacacat gggagatttt cagggcttta gcaagagacc
    65401 tttgggtgtg tttgaggttt ttccgacgat acctgatgcg ttcgccgata gttaaatttt
    65461 ttgtttccat agttagctaa tgctaaatcg tattgactat gtttttgtta acatctatct
    65521 tgttagttat gactaacaat aaaggtgttt taaatgctta aaactgacgc tcttttgtat
    65581 ttcggttcaa aaacaaaact tgcacaagca gcaggtattc gtttggcttc gctttatagc
    65641 tggaaagggg atttagttcc cgaaggtcgc gcgatgcgtc tacaggaggc atctggcggg
    65701 gagcttcagt atgatcccaa agtttatgat gaatatcgta agacgaagcg ggcggggcgg
    65761 ttgaacaatg aaaatcactc ctgaacaggc tcgtgaggct ctggatgcct ggatatgtcg
    65821 accaggaatg acacaggagc aggcgacgat attaatcact gaagcattct gggctttgaa
    65881 agagcgcccg aacatcgatg ttcagcgtgt cacatatgaa ggtggcgcga ttgatcagcg
    65941 agcgcttggc gttaatcgag tgaagatatt tgaacgctgg aaggctatcg acaccaggga
    66001 taagcgtgaa aagttcacgg cgctagtgcc tgcaattatg gaggctatcc ggattaatga
    66061 tttcaggttg tatcgtgaaa ttactgacgg aaaaagcatc acgtgcatga tcgccgggtt
    66121 aaacaaagaa tatggcgatg tggtggagtc cggactgctt tttgctgatc ctgccgtagt
    66181 ggatcgtgaa actgacgaac ttatagaaaa agcaattgct ttcaaacttg cgtatcgaca
    66241 gcaataccaa caaaaagctg gatggaatta tgagccttct ttttgctgaa cgcccactgg
    66301 ttataaacac gcagctggca atgaaaattg gcttaaacga agccattgtt ttgcaacaac
    66361 tgcactactg gttgagagat accaactccg gcatggaatg tgatggtgtt cgctggattt
    66421 ataacacaac ggaacaatgg ctggaacagt tcccattctg gtcagagtca acgttaaagc
    66481 gcgcgtttgc aagtctgaaa acgctggggc ttttgcgttg tgaaaagctc aataaatcaa
    66541 agcgcgatat gaccaatttc tacacgatca actatgggag cgagctttta gatggtggca
    66601 aattgagcga atccatcggt tcaaaatgcg ccgctccatc aggtcaaaat gacacgatgg
    66661 aagaggtcaa aatgaaacgc tccattggtt caaaacgacc caatgtcatc gggtcaaaat
    66721 ggcctgatga tcttacagag aatacaacag agattactac agagaataaa aacacttttc
    66781 gtccggaagc ttcgcaaccg gacccgcaga cgactgaaca ggatttttta acccggaact
    66841 ccgacgcggt tgtgtttagt gcgaaaaaac gccagtgggg tagcagggag gatttggcgt
    66901 gtgcgcagtg gatctggggg cggatcgtgg gtctctacga acaggcagcc agtgatgatg
    66961 gcgagatcat gcgaccaaaa gagcctaact ggactgcctg ggccaatgac gtgcgcacaa
    67021 tgcggatgct ggatggcaga agccacagac aaatttgcga aatgtttggt cgggttcagc
    67081 gagatccatt ctgggtaaaa aacatcatga gcccgtcaaa gctccgcgaa aaatgggacg
    67141 aactggtcat ccgcctggga cgttcacctg tacagcgttg tgttaatcat atttctgaac
    67201 cggatacaga aattccgcct ggtttcagag gataagtgtt gatttcaggt catgaggtaa
    67261 ttttaagggg gacttgtggc aaaagttttt acacaagaag agcgggaaaa aattaaatgg
    67321 caggtggtgg aactcgtgcg ccagagcggc cgtgagacgt tacggcaact ggaagctaaa
    67381 acaggtgcga ctagatatct gatgagcgtt cttgccagag agctggtagc cagtggcgat
    67441 gtatacaact ccggctacgg gttattcccc tctgaacagg cgcgtaagga ctggcaaaac
    67501 gcccgcaaaa aactctcgag ggcaaaggtg aagaaacctg ctgtggttga tccggacctt
    67561 atctggtcat cacctgacgg agaaatacgt cgctacgaca gtcgcctaaa cataatctgt
    67621 cgcgagtgcc ggaagagcga agttatgcag cgcatactgg ctttctatca gggtaatttt
    67681 caggacgtgg cgcagtgagt gcaccggcaa ccattcttga tatgtgctgt ggcagccgca
    67741 tgttctggtt cgataagtct gacgaacggg cgatattcag cgatatcaga aaagaaggat
    67801 acacattacg caatgggaga cgcttgatta tcagccctga cattatcgca gattttcgtg
    67861 cattatcatt tgcagacgca tctttttcga tggttgtatt agaccctccg catcttgaga
    67921 gtgttggtga taacgcctgg atgggaaaga aatatggacg gctgaataaa gatgcctggc
    67981 gtgatgattc gcgacagaga tttaaagaag cctttcgggt gttgaggccg cacggcgttc
    68041 tgatttttta atggaatgaa acgcaaatac cggtaagcca gattctggca ctgacagaca
    68101 gaaaacctgt tatctgtcaa cgaacaggga aaaaacgata aaacccactg gattattttt
    68161 atgaaagagg catccagtga gtagattcgt
//
LOCUS       DBANUU010000005        65192 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000005 DBANUU010000000
VERSION     DBANUU010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.005"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..450)
                     /gene="folK"
                     /locus_tag="ACIE68_000292"
     CDS             complement(<1..450)
                     /gene="folK"
                     /locus_tag="ACIE68_000292"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFX5906668.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHT"
     gene            complement(447..1844)
                     /gene="pcnB"
                     /locus_tag="ACIE68_000293"
     CDS             complement(447..1844)
                     /gene="pcnB"
                     /locus_tag="ACIE68_000293"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFX5906669.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1904..2800)
                     /gene="gluQRS"
                     /locus_tag="ACIE68_000294"
     CDS             complement(1904..2800)
                     /gene="gluQRS"
                     /locus_tag="ACIE68_000294"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFX5906670.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2867..3322)
                     /gene="dksA"
                     /locus_tag="ACIE68_000295"
     CDS             complement(2867..3322)
                     /gene="dksA"
                     /locus_tag="ACIE68_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFX5906671.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3500..4204)
                     /gene="sfsA"
                     /locus_tag="ACIE68_000296"
     CDS             complement(3500..4204)
                     /gene="sfsA"
                     /locus_tag="ACIE68_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFX5906672.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4219..4749)
                     /gene="thpR"
                     /locus_tag="ACIE68_000297"
     CDS             complement(4219..4749)
                     /gene="thpR"
                     /locus_tag="ACIE68_000297"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFX5906673.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4823..7252
                     /gene="hrpB"
                     /locus_tag="ACIE68_000298"
     CDS             4823..7252
                     /gene="hrpB"
                     /locus_tag="ACIE68_000298"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFX5906674.1"
                     /translation="MSSLPVAAVLSELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKDLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7448..9982
                     /gene="mrcB"
                     /locus_tag="ACIE68_000299"
     CDS             7448..9982
                     /gene="mrcB"
                     /locus_tag="ACIE68_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFX5906675.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10202..12391
                     /gene="fhuA"
                     /locus_tag="ACIE68_000300"
     CDS             10202..12391
                     /gene="fhuA"
                     /locus_tag="ACIE68_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFX5906676.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIANDGHTLNRGTVVDNERLQNFSVDTQLESKFATGEVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGNAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRVSYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12442..13239
                     /gene="fhuC"
                     /locus_tag="ACIE68_000301"
     CDS             12442..13239
                     /gene="fhuC"
                     /locus_tag="ACIE68_000301"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFX5906677.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPTEGEILLDTQPLESWSSKAFARKVAYLPQQLPPAEGLTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13239..14129
                     /gene="fhuD"
                     /locus_tag="ACIE68_000302"
     CDS             13239..14129
                     /gene="fhuD"
                     /locus_tag="ACIE68_000302"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFX5906678.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14126..16108
                     /gene="fhuB"
                     /locus_tag="ACIE68_000303"
     CDS             14126..16108
                     /gene="fhuB"
                     /locus_tag="ACIE68_000303"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFX5906679.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLIIAVVVALSFGRDAHGWTWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16143..17423)
                     /gene="hemL"
                     /locus_tag="ACIE68_000304"
     CDS             complement(16143..17423)
                     /gene="hemL"
                     /locus_tag="ACIE68_000304"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFX5906680.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17589..17636
                     /gene="yadX"
                     /locus_tag="ACIE68_000305"
     CDS             17589..17636
                     /gene="yadX"
                     /locus_tag="ACIE68_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFX5906681.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17648..19069
                     /gene="clcA"
                     /locus_tag="ACIE68_000306"
     CDS             17648..19069
                     /gene="clcA"
                     /locus_tag="ACIE68_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFX5906682.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19093..19158
                     /gene="yadW"
                     /locus_tag="ACIE68_000307"
     CDS             19093..19158
                     /gene="yadW"
                     /locus_tag="ACIE68_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFX5906683.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19151..19495
                     /gene="erpA"
                     /locus_tag="ACIE68_000308"
     CDS             19151..19495
                     /gene="erpA"
                     /locus_tag="ACIE68_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFX5906684.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19542..20165)
                     /gene="yadS"
                     /locus_tag="ACIE68_000309"
     CDS             complement(19542..20165)
                     /gene="yadS"
                     /locus_tag="ACIE68_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFX5906685.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20203..21003)
                     /gene="btuF"
                     /locus_tag="ACIE68_000310"
     CDS             complement(20203..21003)
                     /gene="btuF"
                     /locus_tag="ACIE68_000310"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFX5906686.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20996..21694)
                     /gene="mtnN"
                     /locus_tag="ACIE68_000311"
     CDS             complement(20996..21694)
                     /gene="mtnN"
                     /locus_tag="ACIE68_000311"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFX5906687.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIEKRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21778..23295
                     /gene="dgt"
                     /locus_tag="ACIE68_000312"
     CDS             21778..23295
                     /gene="dgt"
                     /locus_tag="ACIE68_000312"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFX5906688.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCYFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23425..24849
                     /gene="degP"
                     /locus_tag="ACIE68_000313"
     CDS             23425..24849
                     /gene="degP"
                     /locus_tag="ACIE68_000313"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFX5906689.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            25004..26161
                     /gene="cdaR"
                     /locus_tag="ACIE68_000314"
     CDS             25004..26161
                     /gene="cdaR"
                     /locus_tag="ACIE68_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFX5906690.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26250..26636)
                     /gene="yaeH"
                     /locus_tag="ACIE68_000315"
     CDS             complement(26250..26636)
                     /gene="yaeH"
                     /locus_tag="ACIE68_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFX5906691.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26951..27775)
                     /gene="dapD"
                     /locus_tag="ACIE68_000316"
     CDS             complement(26951..27775)
                     /gene="dapD"
                     /locus_tag="ACIE68_000316"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFX5906692.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27806..30478)
                     /gene="glnD"
                     /locus_tag="ACIE68_000317"
     CDS             complement(27806..30478)
                     /gene="glnD"
                     /locus_tag="ACIE68_000317"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFX5906693.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30540..31334)
                     /gene="map"
                     /locus_tag="ACIE68_000318"
     CDS             complement(30540..31334)
                     /gene="map"
                     /locus_tag="ACIE68_000318"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFX5906694.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31702..32427
                     /gene="rpsB"
                     /locus_tag="ACIE68_000319"
     CDS             31702..32427
                     /gene="rpsB"
                     /locus_tag="ACIE68_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFX5906695.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32685..33536
                     /gene="tsf"
                     /locus_tag="ACIE68_000320"
     CDS             32685..33536
                     /gene="tsf"
                     /locus_tag="ACIE68_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFX5906696.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33683..34408
                     /gene="pyrH"
                     /locus_tag="ACIE68_000321"
     CDS             33683..34408
                     /gene="pyrH"
                     /locus_tag="ACIE68_000321"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFX5906697.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34558..35115
                     /gene="frr"
                     /locus_tag="ACIE68_000322"
     CDS             34558..35115
                     /gene="frr"
                     /locus_tag="ACIE68_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFX5906698.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35207..36403
                     /gene="ispC"
                     /locus_tag="ACIE68_000323"
     CDS             35207..36403
                     /gene="ispC"
                     /locus_tag="ACIE68_000323"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFX5906699.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSCTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36592..37350
                     /gene="ispU"
                     /locus_tag="ACIE68_000324"
                     /gene_synonym="uppS"
     CDS             36592..37350
                     /gene="ispU"
                     /locus_tag="ACIE68_000324"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFX5906700.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37363..38220
                     /gene="cdsA"
                     /locus_tag="ACIE68_000325"
     CDS             37363..38220
                     /gene="cdsA"
                     /locus_tag="ACIE68_000325"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFX5906701.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38232..39584
                     /gene="rseP"
                     /locus_tag="ACIE68_000326"
     CDS             38232..39584
                     /gene="rseP"
                     /locus_tag="ACIE68_000326"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFX5906702.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39614..42046
                     /gene="bamA"
                     /locus_tag="ACIE68_000327"
     CDS             39614..42046
                     /gene="bamA"
                     /locus_tag="ACIE68_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFX5906703.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42168..42653
                     /gene="skp"
                     /locus_tag="ACIE68_000328"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42168..42653
                     /gene="skp"
                     /locus_tag="ACIE68_000328"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFX5906704.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42657..43682
                     /gene="lpxD"
                     /locus_tag="ACIE68_000329"
     CDS             42657..43682
                     /gene="lpxD"
                     /locus_tag="ACIE68_000329"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFX5906705.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43787..44242
                     /gene="fabZ"
                     /locus_tag="ACIE68_000330"
     CDS             43787..44242
                     /gene="fabZ"
                     /locus_tag="ACIE68_000330"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFX5906706.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44246..45034
                     /gene="lpxA"
                     /locus_tag="ACIE68_000331"
     CDS             44246..45034
                     /gene="lpxA"
                     /locus_tag="ACIE68_000331"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFX5906707.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45034..46182
                     /gene="lpxB"
                     /locus_tag="ACIE68_000332"
     CDS             45034..46182
                     /gene="lpxB"
                     /locus_tag="ACIE68_000332"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFX5906708.1"
                     /translation="MTKQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46179..46775
                     /gene="rnhB"
                     /locus_tag="ACIE68_000333"
     CDS             46179..46775
                     /gene="rnhB"
                     /locus_tag="ACIE68_000333"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFX5906709.1"
                     /translation="MIEFVYPHTHLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            46810..50292
                     /gene="dnaE"
                     /locus_tag="ACIE68_000334"
     CDS             46810..50292
                     /gene="dnaE"
                     /locus_tag="ACIE68_000334"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFX5906710.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50305..51264
                     /gene="accA"
                     /locus_tag="ACIE68_000335"
     CDS             50305..51264
                     /gene="accA"
                     /locus_tag="ACIE68_000335"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFX5906711.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51363..53504
                     /gene="ldcC"
                     /locus_tag="ACIE68_000336"
     CDS             51363..53504
                     /gene="ldcC"
                     /locus_tag="ACIE68_000336"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFX5906712.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53561..53950
                     /gene="yaeR"
                     /locus_tag="ACIE68_000337"
     CDS             53561..53950
                     /gene="yaeR"
                     /locus_tag="ACIE68_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFX5906713.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54015..55313
                     /gene="tilS"
                     /locus_tag="ACIE68_000338"
     CDS             54015..55313
                     /gene="tilS"
                     /locus_tag="ACIE68_000338"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFX5906714.1"
                     /translation="MTLTLNRQLLTLRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDIAHCQKPQGTLQIAPMLAMSDARRAAIIRRWLAGKNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGEKGVSFVWQKTLS"
     gene            complement(55362..55622)
                     /gene="rof"
                     /locus_tag="ACIE68_000339"
     CDS             complement(55362..55622)
                     /gene="rof"
                     /locus_tag="ACIE68_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFX5906715.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55609..55809)
                     /gene="yaeP"
                     /locus_tag="ACIE68_000340"
     CDS             complement(55609..55809)
                     /gene="yaeP"
                     /locus_tag="ACIE68_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFX5906716.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            55975..56520
                     /gene="yaeQ"
                     /locus_tag="ACIE68_000341"
     CDS             55975..56520
                     /gene="yaeQ"
                     /locus_tag="ACIE68_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFX5906717.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKFVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56517..56939
                     /gene="arfB"
                     /locus_tag="ACIE68_000342"
     CDS             56517..56939
                     /gene="arfB"
                     /locus_tag="ACIE68_000342"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFX5906718.1"
                     /translation="MIVISLHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            56953..57663
                     /gene="nlpE"
                     /locus_tag="ACIE68_000343"
                     /gene_synonym="cutF"
     CDS             56953..57663
                     /gene="nlpE"
                     /locus_tag="ACIE68_000343"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFX5906719.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(57818..58642)
                     /gene="yaeF"
                     /locus_tag="ACIE68_000344"
     CDS             complement(57818..58642)
                     /gene="yaeF"
                     /locus_tag="ACIE68_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFX5906720.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSADFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(58695..60413)
                     /gene="proS"
                     /locus_tag="ACIE68_000345"
     CDS             complement(58695..60413)
                     /gene="proS"
                     /locus_tag="ACIE68_000345"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFX5906721.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60524..61231)
                     /gene="tsaA"
                     /locus_tag="ACIE68_000346"
     CDS             complement(60524..61231)
                     /gene="tsaA"
                     /locus_tag="ACIE68_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFX5906722.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLMAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61228..61632)
                     /gene="rcsF"
                     /locus_tag="ACIE68_000347"
     CDS             complement(61228..61632)
                     /gene="rcsF"
                     /locus_tag="ACIE68_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFX5906723.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61750..62565)
                     /gene="metQ"
                     /locus_tag="ACIE68_000348"
     CDS             complement(61750..62565)
                     /gene="metQ"
                     /locus_tag="ACIE68_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFX5906724.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62605..63258)
                     /gene="metI"
                     /locus_tag="ACIE68_000349"
     CDS             complement(62605..63258)
                     /gene="metI"
                     /locus_tag="ACIE68_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFX5906725.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63251..64282)
                     /gene="metN"
                     /locus_tag="ACIE68_000350"
     CDS             complement(63251..64282)
                     /gene="metN"
                     /locus_tag="ACIE68_000350"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFX5906726.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64470..65042
                     /gene="gmhB"
                     /locus_tag="ACIE68_000351"
     CDS             64470..65042
                     /gene="gmhB"
                     /locus_tag="ACIE68_000351"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFX5906727.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15452 a        16180 c        17909 g        15651 t
ORIGIN      
        1 tgtatgtaag acttcacgca acgtctcccc atcaggaaac gccagctccg gtgcaatctc
       61 aaacagcggc cacagcataa atccacgatt cttcatatcg tagtgcggaa cggtcaggcg
      121 ttcggtattt atcacttcat taccaaacag catgatatcg agatccagcg tgcgtggtcc
      181 ccagcgttca gctttacgaa cacgaccctg ttgcagttct atgcgctgtg tatggttgag
      241 taactcttcc ggggcgagcg aagtttcaag cgccacggcg gcgttgaggt aatcgggttg
      301 atcttgcggc cccagcggtg gggtgcggta aaacgaagaa acggcaagaa tgtggctttc
      361 agggatatcg cctaatgctt tcagggcagc attgacctgc tccagcggag aggccagatt
      421 gctgcctatg gcaatatacg ccactgtcat gcggtaccct cacgacgcgg tgcgcgtttg
      481 cgtggacgac gagtacggcg acgcggtgac ggctcttcat ccaactcgtt aagcatccct
      541 ttttgatctg gtggcgcgga aacctggaac tcaccccacc atttcaccag acgctgcagt
      601 tcagcgttac gttcaacttc agctcgcaag gccaacaggt cataagccgc acggaactta
      661 ggatgctcca gcagtttcca tgcgcgttta ccctgacgac gggacatacg caactgcaac
      721 tgccagatat cgcgggttaa cgtcgtcaga cgtttcggga ttgccagtga acggcaggct
      781 tcgtccagca cgtcgttcat cgccagcgcg aaagcgtcgt gataggtcag gccgctttcc
      841 tgggcaatct tctgtgccgt ctccagcagt gggtaccaga acatggcggc aaacaggaac
      901 gccgggttca cgcgcatatc gttatggata cgcgtatcgg tattcttcag cacctgttca
      961 atgatccgct ccatcgggct gtcgccattt tccgtgaagt agcgggtaat ggtcgggaac
     1021 agcggctgga acagatgata ttcacacaac agcttatagg tatcgtaacc gtagcccgct
     1081 tgtagcagtt taagcgattc ttcaaacagg cgtgccggtg ggatatcgtt caacagggta
     1141 gcgaggcgag ggatcggttc tgcggtttcc gggctgatgc gcatacccaa tttggcggca
     1201 aaacgtaccg cgcgcagcat acgtaccgga tcttcacggt agcgcgtttc cgggttacca
     1261 atcagacgga taacgccgtc cttcagatcc ttcataccgc caacgtaatc acggacggta
     1321 aaatccgcta cgctgtaata gaggctgttg atagtgaaat cgcggcgctg ggcgtcttct
     1381 tcgatggagc cgaaaatgtt gtcgcgcagc aacatgccgt tttgcccgcg ttgggaggtc
     1441 gtgcggtcgc tgacgttacc ttcgtggtgt ccacggaagg tcgcaacttc gataatctcc
     1501 gggccaaaca ttacatgagc cagacggaaa cggcgaccca ccaggcggca gttacggaac
     1561 agtttgcgca cctgctcagg cgtggcgtta gtggttacgt caaaatcttt cggctttttg
     1621 ccaagtaaca ggtcgcgcac gccgccgcca accagccagg cttcgtatcc cgctttgttg
     1681 agcctgtaca ttaccttcag ggcattttca ctgatatctt tgcgggaaat agcatgctgc
     1741 tcacgcggga tcaccgtcac ctgtggacgg gcgactgcct gttcagcctc gctttcctcg
     1801 cggcttagca ccttgcggca aaaattagcg actcgggtaa aaatagtaca cctcggtagt
     1861 gtcaaacatc attcaggaca aaaaaatagc ggctaatcat agctcagcat gacgcatttg
     1921 agaatgttga atttacaatt gccgactcgg gcacggcggt aagccgccag tttttgacgg
     1981 ctgactgaag gatttgctcg acgctgaaat cctgccagtg tgcttctgcc tgctgcccca
     2041 gaaattgaag tgccgcgatt agtaccgggc gtggatcgcc tttcggcaac gcaggcgcat
     2101 gattctgctt ggaaagttta gcgccttgtg gattaagcgc cagcggcaga tgaatgtaat
     2161 ctggcacttt ccagccaaaa agctggtaca gcgagatttg ccttactgtt ggttcaatca
     2221 gatcagcccc acgcactatt tctgtaacgc cctggaaatg atcatcaacc acaacagcca
     2281 ggttgtaggc gaacaaccca tcacggcgat gaatgataaa atcttcccgt gccagttttt
     2341 cgtcggcgtg aataatgccg cgcagctggt cagtaaattg cgtgaccgga tgctgctggc
     2401 ggatacgcac tgcggcgttg tctggtccat gatgcaacac ccggcaatga ccgtcgtaaa
     2461 taccgccaat gctttgaata cgcgcacgcg tacaggtgca gtaataactt agtccttgtt
     2521 catgtaacca ggcgagtgct tcacgatagg cgtcgtgacg ttgcgattgc cagagaacat
     2581 cgccgtccca gtgcagaccg taatgttcca gctggcgcag gatagtttct gcggcaccgg
     2641 gaacttcacg aggcgggtcg atatcttcta tgcgtaccag ccagcgacct tgccgggcgc
     2701 gagcctgcaa atagctgccg agcgcggcga tcagagagcc aaaatgaagc tcgccggaag
     2761 gagagggggc gaagcggcca atatactgtg tgtctgtcat ctctttgaac aaaaaataag
     2821 gcgggagcat ttcccgcctg tggtaaacgt gatggaacgg ctgtaattag ccagccatct
     2881 gtttttcgcg aatttcagcc agcgttttgc agtcgatgca cagatcggct gtcgggcgcg
     2941 cttccagacg gcgaatacca atttcaacac cgcaggattc gcagtagccg aaatcttcgt
     3001 cttccacttt tttcagcgtc ttctcgatct ttttgatcag cttacgctcg cgatcgcggt
     3061 tacgcagttc gaggctgaac tcttcttcct gggctgcacg gtctaccggg tccgggaagt
     3121 tggctgcttc atcctgcata tgtgtaacgg tgcgatcgac ttcatccctg agttgattac
     3181 gccatgcttc cagaatacga cggaagtgcg ccagctgggc ttcattcata tactcttcgc
     3241 ccggcttctc ctgatatggt tccaccccag cgatggcgag aatactcagg gacgatgttt
     3301 tacggttttg cccttcttgc atgttgcttc tccttaacac gcactatcga tccccatgtt
     3361 cgggggaaaa atgaggccgc tataaatagc agatgctttt ccggatagca attatctaaa
     3421 cgtaacactt gacaactgtg tgaggaaaag cgtatttgcg cacgcgacca gaatgtaaat
     3481 taaccagtta cttactttac tacaatgtaa ccggcagtga ttttttaaga gccatgcctt
     3541 cagcagaaat ttccgctttg taagccagaa tttctacccc cctctgttga gcttctgaca
     3601 atagttgcgc gtatttctca tcgatgtggc gcgcgggtga aaaccgtgta atggctgaat
     3661 gcagcacggc gaaaaagata accgcacgct ggccttcagc cgctacgctc atcaactccc
     3721 gaaggtgttt ctgacctcgt tcagtgaccg catcgggaaa atatccctgt tcgttctccg
     3781 ctaacgtaac cgatttcact tcaatatagc agtctggacg cgaatccgcc tgcaacataa
     3841 agtcaatacg gctgcgttct gcgccgtatt ttacttcgct tttcagcgag ctatagcctg
     3901 acagttctga aattgattca ttaaggatag cctctttcgt caacctgtta gcccaaagcg
     3961 tgttgacgca aataaatgcg ccgctctggc tttgagttaa ttcccaggtg tgtgggtatt
     4021 tccgtttggt gttgtctgaa gtcgaatacc agacggtatc gccaggcgtt gcacaaccgg
     4081 tcattgcacc cgtattcggg cagtgtagcg ttaattcgcg accatcgggt gtgatcacat
     4141 cggctaaaaa acgtttgtaa cgctgaatta gcgtcgcgcg ctgtagaggg ggagagaatt
     4201 ccattgcgac aatccttgtt attgcgttag cgcccagcgt tttagcggcg tgtagcgtgt
     4261 gcgtccacgg gcaaacgagg aggcgtaaag ggtgaactcc gtcaccgcat aagaccagtt
     4321 aaaacctggc ggcgggattg tcaccgcctc gctggcgtcg cgcaataagg taatatgtgg
     4381 atgaaacgga cgattgcttt gaaaacaacc gctgcgggca gcctgtgaac ggagcatatt
     4441 cgccagctgg attaagccgc gtggaggctg acgcatccct aaccacacca cacgcgaacg
     4501 cagccattgt ccggcgtcat caagcgtgag tgtgaaacca ggttgacgaa tccgtccggc
     4561 taaaagagaa agcgccttct ctttctcggc gctcacttcg cctaaaaatg ccagagtcag
     4621 atgcaaatta tcggcggcga ccggacgtcc cgcctcaggt gggaagtgtg tggcgcgcca
     4681 atggataatc tgttcgcgga tttctgcagg taagtcgata gcaaagaaca gacgttgcgg
     4741 ttcagacatg tgaggcactc ggttatgaat taccgcgatg ctacaatgtg gcgcgaagaa
     4801 tgttaaccct ctggagcgtt ttgtgtcgtc gttgcccgtt gctgccgtct tatctgaatt
     4861 acttaccgct ctcgattgtg cgccgcaggt attattaagt gcgccgaccg gggccgggaa
     4921 atcaacctgg ctgccgctgc aactgctggc gcatcccggc attaacggga aaattatcct
     4981 gctggagccg cgtcgtctgg cggcgcgtaa cgtcgcgcaa cggctggcgg agctgcttaa
     5041 cgaaaagcca ggcgataccg ttggctaccg gatgcgtgcg caaaactgcg tcgggccgaa
     5101 tacccgcctg gaagtggtta ccgaaggcgt gctgacgcgc atgatccagc gtgacccgga
     5161 actgagcggt gttggactgg tgatccttga tgaatttcat gagcgcagct tgcaggcgga
     5221 tttggcgttg gcgctgttac tcgatgtgca acaaggtctg cgtgatgacc ttaaactgct
     5281 gattatgtcg gctaccctgg acaacgaccg cttgcagcaa atgctgccag aagcgcctgt
     5341 cgtcatctca gaagggcgct cgtttccggt tgaacgccgt tatttaccgc tgcccgcgca
     5401 tcagcgtttt gacgatgccg ttgcggtagc caccgctgaa atgctgcgtc aggaaagcgg
     5461 atcattactg ttatttttac ctggcgtcgg agaaattcag cgtgtgcagg aacaactggc
     5521 ttcgcgcatc ggcagtgatg tattgctctg cccgctgtat ggcgcgttgt cgctgaacga
     5581 tcagcgaaaa gcgatcctcc cggcaccgca agggatgcgc aaagtggtgc tggcgaccaa
     5641 tattgctgaa accagtttaa ccattgaagg tattcgtctg gtggtggatt gtgcccagga
     5701 gcgtgtggcg cgttttgatc cgcgcacggg gcttacgcga ctgattactc aacgcgttag
     5761 ccaggcatcc atgacgcagc gtgccgggcg cgccgggcgt ctggagccgg gtatctgcct
     5821 gcatttaatc gccaaagaac aagcagaacg cgccgcggcg caaagtgaac cggagatctt
     5881 acaaagcgat ctttccggtt tgctgatgga attactgcaa tggggatgca gcgatccggc
     5941 gcagatgagc tggctggatc aaccgccagt agtgaatcta ctggccgcga aacgtctgtt
     6001 acaaatgctg ggggcactgg agggtgaacg gcttagtgcg caagggcaaa aaatggcagc
     6061 gctgggtaac gatccgcgtt tagcggcaat gctggttagc gcgaagaacg acgacgaagc
     6121 tgctaccgcg gcaaaaattg ccgccattct cgaagagccg ccacggatgg gcaatagtga
     6181 cctgggcgtg gcgttttcgc gcaatcaacc agcctggcag caacgtagtc agcaactgtt
     6241 aaaacgctta aacgtacgtg gcggtgaggc agacagttcg cttatcgcgc cgctacttgc
     6301 cggggcgttt gccgatcgca ttgctcgtcg ccgtgggcaa gatggacgct atcaactggc
     6361 aaacggcatg ggagcgatgc tcgatgccaa cgacgcgcta agccgccacg aatggttgat
     6421 cgcaccgtta ttattgcagg gcagcgcctc gccggatgcg cggattttac tggcgctgcc
     6481 ggtcgatatt gatgagttag tacaacgctg cccgcagctg gtacagcagt ctgacactgt
     6541 ggagtgggat gacgcgcaag gtacgctgaa agcctggcgt cggctgcaaa tcggtctgct
     6601 gacggtgaaa gtgcagccgc tggcgaaacc ctcggaagac gagttgcatc aggcgatgct
     6661 taatggcatc cgcgataaag atttaagcgt gctcaactgg acggcggaag cggaacagct
     6721 acgcttgcgt ttgttatgcg ccgcaaagtg gttgccggaa tatgactggc cagcggttga
     6781 tgatgaaagt ttattggcga cgctggaaac gtggctgctg ccacatatga ctggcgtaca
     6841 ttcactacgc ggcctgaaat cactcgacat ttatcaggca ctacgcggat tacttgattg
     6901 gggaatgcag caacgtctgg atagtgaatt gcctgcgcat tacactgtgc cgacgggaag
     6961 ccggatcgcc attcgttatc atgaagataa cccgcccgcg ctggcggtga gaatgcaaga
     7021 gatgtttggc gaggccacca atccgacgat cgcccagggg cgcgtgccgc tggtgctgga
     7081 gttgctttca cctgcccaaa ggccattaca aatcacacga gatttgagcg ccttctggaa
     7141 aggagcgtac cgtgaggtgc aaaaagagat gaaagggcgt tatcccaaac atgtctggcc
     7201 ggacgacccg gcaaatactg caccgacgcg acggacgaaa aagtattcgt aaggttggtt
     7261 ttctccctct ccctgtggga gagggccggg gtgagggcat cagcgcgcac gttcaccctc
     7321 accctaaccc tctccctcaa gggagagggg accgatcgag cacaaatttt gagagatatc
     7381 ttcttctgtc ttgtaacaga agaacagaaa atcgggcttt tgcgcctgaa tattgcggag
     7441 aaaaagcatg gccgggaatg accgcgagcc aattggacgc aaagggaaac cgacgcgtcc
     7501 ggtcaaacaa aaggtaagcc gtcgtcgtta cgaagatgac gatgattacg acgattatga
     7561 tgactatgag gatgaagaac cgatgccgcg caaaggtaag ggcaaaggca aagggcgtaa
     7621 gcctcgtggc aaacgcggct ggctatggct gctgttaaaa ctggctatcg tttttgccgt
     7681 gctgatcgcc atttacggcg tttatctcga tcaaaaaatt cgtagccgta ttgatggcaa
     7741 ggtctggcaa ctgcctgcgg cagtttatgg ccgaatggtc aaccttgagc cagatatgac
     7801 catcagcaag aacgagatgg tgaagctgct ggaggcgact cagtatcgtc aggtgtcgaa
     7861 aatgacccgt cctggcgaat ttaccgtgca ggccaacagc attgagatga tccgccgtcc
     7921 gtttgatttc ccggacagta aagaagggca ggtgcgtgcg cgtctgacct ttgatggcga
     7981 tcatctggcg acgatcgtca atatggagaa caaccgtcag ttcggtttct tccgtcttga
     8041 tccgcgtctg atcaccatga tctcctcgcc aaacggtgag cagcgtttgt ttgtgccgcg
     8101 cagtggtttc cctgatttgc tggtggatac tttgctggcg acagaagacc gtcattttta
     8161 cgagcatgac ggcatcagcc tctactccat cggacgtgcg gtgctggcaa acctgaccgc
     8221 cggacgcacg gtgcagggag cgagtacgct gacgcaacag ctggtgaaaa acctgttcct
     8281 ctccagtgag cgttcttact ggcgtaaagc gaacgaagct tacatggcgc tgatcatgga
     8341 cgcgcgttac agcaaagacc gtattcttga gctgtatatg aacgaggtgt atctcggtca
     8401 gagcggcgac aacgaaatcc gcggcttccc gctggcgagc ttgtattact ttggtcgccc
     8461 ggtagaagag ctaagcctcg accagcaggc gctgttagtc ggcatggtga aaggagcgtc
     8521 catctacaac ccgtggcgta acccaaaact ggcgctggag cgacgtaatc tggtgctgcg
     8581 tctgctgcaa cagcaacaga ttattgatca agaactctat gacatgttga gtgcacgtcc
     8641 gctgggtgtt cagccacgcg gtggggtgat ctctccacag ccagccttta tgcaactggt
     8701 gcgtcaggag ctgcaggcga aattaggcga taaggtaaaa gatctctccg gcgtgaagat
     8761 cttcactacc tttgactcgg tggcccagga cgcggcagaa aaagccgccg tggaaggcat
     8821 tccggcactg aagaaacagc gtaagttgag cgatcttgaa actgcgattg tggtcgtcga
     8881 ccgctttagt ggtgaagttc gtgcgatggt cggtggttct gagccgcagt ttgcgggcta
     8941 caaccgtgcg atgcaggcgc gtcgttcgat tggttccctt gcaaaaccag cgacttatct
     9001 gactgcctta agccagccga aaatctatcg tctgaatacg tggattgcgg atgcgccaat
     9061 cgcgctgcgt cagccgaatg gccaggtctg gtcaccgcag aatgatgacc gtcgttatag
     9121 cgaaagcggc agagtgatgc tggtggatgc gttgacccgt tcgatgaacg tgccgacggt
     9181 aaatctgggg atggcgctgg ggctgcctgc ggttacggag acctggatca aactgggcgt
     9241 accgaaagat cagttgcatc cggttccggc gatgctgctg ggggcgttga acttaacgcc
     9301 aatcgaagtg gcgcaggcgt tccagaccat cgccagcggc ggtaaccgtg caccgctttc
     9361 tgcactgcgt tcggtaattg cggaagatgg caaggtgctg tatcagagct tcccgcaggc
     9421 ggaacgcgct gttccggcgc aggcggcgta tctgacacta tggaccatgc agcaggtggt
     9481 acaacgcggt acgggtcgtc agcttggggc gaaatacccg aacctgcatc tggcagggaa
     9541 aacagggact accaacaata acgtagatac ctggtttgcg ggcattgacg gcagcacggt
     9601 gaccatcacc tgggtaggcc gtgataacaa ccagccgacc aaactgtatg gtgccagcgg
     9661 ggcaatgtcg atttatcagc gttatctggc taaccagacg ccaacgccgc tgaatcttgt
     9721 tccgccagaa gatattgcag atatgggcgt ggactacgac ggcaactttg tttgcagcgg
     9781 tggcatgcgt gtcttgccgg tctggaccag cgatccgcaa tcgctgtgcc agcagagtga
     9841 gatgcagcag caaccgtcag gcaatccgtt tgatcagtct tctcagccgc agcaacagcc
     9901 gcaacagcaa cctgctcagc aagagcagaa agacagcgac ggtgtagccg gttggatcaa
     9961 ggatatgttt ggtagtaatt aacatctaag cgtgaaatac cggatggcga attgccatcc
    10021 ggtaaaataa catcccacct aaagtattaa ccctttcttt tcatctggtt gtttattaac
    10081 ccttcaggaa cgctcagatt gcgtaccgct tgcgaacccg ccagcgtttc gaatattatc
    10141 ttatctttat aataatcatt ctcgtttacg ttatcattca ctttacatca gagatatacc
    10201 aatggcgcgt tccaaaactg ctcagccaaa acactcactg cgtaaaatcg cagttgtagt
    10261 agccacagcg gttagcggca tgtctgttta tgcacaggca gcggttgaac cgaaagaaga
    10321 cactatcacc gttaccgctg cacctgcgcc gcaagaaagc gcatgggggc ctgctgcaac
    10381 tattgcggcg aagcactctg ctactgcgac taaaacggat acaccaattg aaaaaacgcc
    10441 gcagtctatt tcggttgtga caaatgaaga gatgcagatg catcaatttc agtctgtaaa
    10501 agaagcatta ggttatacac ctggcgttac ggttagcagt cgtggtgctt ctaatacata
    10561 tgattttgtt atcattcgtg gtttctcatc tgttggtctg aatcaaaata attaccttga
    10621 tggactaaaa cttcagggca acttttataa cgatgctgtg attgatcctt acatgctcga
    10681 gagggttgaa ctgatgcgtg ggccgacgtc tgttctctat ggaaaaagta atcctggtgg
    10741 gattatttct atggtgagta agcgtccgac cactgaaccc ctgaaagaaa ttcagtttaa
    10801 aatggggacg gataatctgt ttcagactgg attcgatttc agtgatgcac tggatgataa
    10861 cggtgagttc tcctaccgtt tgacgggcct cgctcgttca acaaacgaac agcagaaaaa
    10921 ttctgaatcc cagcgttata ccattgctcc atcattctca tggcgtccag acgacaaaac
    10981 caattttact ttcctgtcct atttccagaa tgaaccagaa acgggttatt acggttggtt
    11041 gccgaaagag gggaccgttg agccattgcc taatggtaaa cgtctaccga ctgacttcaa
    11101 tgaaggtgca tcgaataata catactcccg taaccagaaa atggtgggat atagttttga
    11161 acatggtttt aatgacacct tcaccgtgcg tcagaatctg cgtttcagtg aaatgaaaac
    11221 ctcacagaaa agtgtttatg gcacagggat tgccaacgat ggtcatactc taaaccgcgg
    11281 gacagtggtg gataatgagc gtctgcaaaa ctttagcgtt gatacccaac ttgaaagtaa
    11341 atttgctaca ggtgaagtgg agcatacttt gctgacaggg gtagacttca tgcgtatgcg
    11401 caatgatatc aatgccagct ttggatctgc accatcaatc gatctttata acaaatatca
    11461 tcctgaatac tttgcatttg gtaacgcaga gccataccaa atgaatgaaa gcaaacaaac
    11521 aggtatttat gttcaggatc aggcggaatg gaataaatgg gtattcactc tggggggacg
    11581 ctacgattgg tctaagcaag cgaccactgt tcgtgaaaac tcttatacgc cgactgaagg
    11641 ttatattgag cgtaatgatc atcagttcac ctggcgcggt ggtgtaaatt acttattcga
    11701 taatggtatt tcaccttact ttagctatag ccagtccttt gaaccgagtg ctttcgatct
    11761 gtggagcaac ccgcgcgttt cctataagcc atcgaaaggt gaacagtatg aagctggcgt
    11821 aaaatatgtt ccgaatgata tgccggtcgt tgttacgggc gcagtctatc aattgacgaa
    11881 aacaaataac ctgacagcag acccaacaaa cccgttagcg caagtcccag caggtgagat
    11941 tcgcgctcgt ggtgtggagc ttgaagcaaa agcagcgtta aatgccaata ttaacttgac
    12001 ggcttcttat acctacacgg atgcggaata caccaaagac accaatctca aaggtaaaac
    12061 tccagaacaa gtaccggagc atatggcatc tctctggggg gattatacct tcaatgaagg
    12121 gccgctttct ggtttaacat tgggaacagg tggtcgtttt attggttcca gctatggtga
    12181 tccggcaaac acttttaaag tgggtagcgc agctgtaatg gatgctgttg taaaatatga
    12241 tctggcacgc tttggtatgg cgggatccag ccttgctgtg aacgtcaaca atttgctcga
    12301 tcgtgagtat gttgccagtt gcttccagac ttatggctgc ttctggggcg cagaacgtca
    12361 ggtcgttgca accgcaacct tccgtttcta atttctcttt tggggcacgg attaccgtgc
    12421 ccatttcaca agttggctgt tatgcaggaa tacacgaatc attccgatac cacttttgca
    12481 ctgcgtaata tctcctttcg tgtgcccggg cgcacgcttt tgcatccgct gtcgttaacc
    12541 tttcctgccg ggaaagtgac cggtctgatc ggtcacaacg gttctggtaa atccactctg
    12601 ctcaaaatgc ttggccgtca tcagcctccg acggaagggg agattcttct tgatacccag
    12661 ccgctggaaa gctggagcag caaagcgttc gcccgcaaag tggcttattt gccgcagcag
    12721 cttcctccgg cagaagggtt gaccgtgcgt gaactggtgg cgattggtcg ttacccgtgg
    12781 catggcgcgc tggggcgctt tggggcggca gatcgcgaaa aagtcgagga agctatctcg
    12841 ctggttggct taaaaccgct ggcgcatcgg ctggtcgata gtctctctgg cggcgaacgt
    12901 caacgggcgt ggatcgccat gctggtggcg caggatagcc gttgtctgtt gctcgacgaa
    12961 ccgacttcgg cgctggatat cgcccaccag gttgatgtgc tggcgctggt gcaccgttta
    13021 agtcaggaac gtggcctgac ggtcattgcc gtgctgcacg atatcaatat ggcggcacgc
    13081 tactgtgatt atctggtcgc cctgcgcggt ggtgaaatga ttgctcaggg aacgcctgcg
    13141 gaaattatgc gcggcgaaac cctcgaaatg atttatggca tcccgatggg tattttgccg
    13201 catccggcgg gtgctgcacc tgtgagtttt gtttattgat gagcggctta cctcttattt
    13261 cgcgccgtcg actgttaacg gcgatggcgc tttctccgtt gttatggcag atgaataccg
    13321 cccacgcggc ggctattgac cccaatcgta ttgtggcgct ggagtggttg ccggtggaat
    13381 tactgctggc gctcggcatc gtgccttacg gcgtggcgga taccatcaac tatcgcctgt
    13441 gggtcagcga accaccattg ccggactcag tgattgacgt cggtttgcgt acagaaccta
    13501 accttgaact gctgaccgaa atgaaaccgt cgtttatggt ctggtcggca ggatatggcc
    13561 cttcatcaga aatgctggca cgtattgcgc cgggtcgcgg atttaacttc agtgacggca
    13621 aacagccgtt ggcgatggcg cgtaaatcgc tgacggaaat ggcagattta cttaacctgc
    13681 aaagcgcagc ggaaacgcat ttagcgcaat acgaagactt tatccgcagc atgaaacccc
    13741 gctttgtgaa gcgtggtgca cgcccgttac tgctgacgac gcttatcgat ccgcgccata
    13801 tgctggtctt cggtccaaac agcttgttcc aggaaattct cgatgagtac ggcatcccaa
    13861 atgcctggca aggggaaacc aacttctggg gcagtaccgc cgtcagtatc gatcgtctgg
    13921 cggcgtataa agacgttgat gtgctctgtt ttgatcacga caacagcaaa gacatggatg
    13981 cgctaatggc aaccccgctg tggcaggcca tgccgtttgt ccgtacaggg cgctttcagc
    14041 gcgtacccgc agtctggttt tatggtgcaa cgctttcggc aatgcacttt gtgcgtgttc
    14101 tggataacgc catcggaggt aaagcgtgag taaacgaatt gcgcttttcc cggcgttatt
    14161 gctggcgctg ttagtgattg tcgctacggc gctaacctgg atgaacttct cacaggcgct
    14221 gccgcgcagt cagtgggcgc aggccgcctg gtcgccggat attgacgtca tcgagcagat
    14281 gatttttcac tacagcttgt tgccgcgtct ggcgatttcg ctgctggtgg gcgcaggcct
    14341 ggggctggtg ggcgtgctgt ttcagcaagt gctgcgtaat ccgctggcgg aaccgacgac
    14401 acttggcgtt gctacaggcg cgcaactggg aattaccgtc accacacttt gggcgatccc
    14461 aggtgctatg gcgagccagt ttgccgcact ggtgggcgct tgtgtcgttg gcttaatcgt
    14521 ctttggcgtc gcgtggggga aacggctttc gccggttacg cttattctcg cggggctggt
    14581 ggtgagcctt tattgcgggg caattaatca gctactggtt atcttccatc acgaccagct
    14641 gcaaagcatg ttcctgtgga gcaccggaac gctgacgcaa accgactggg gcggcgttga
    14701 gcgtttatgg ccgcagttgc tgggcggcgt gatgttgacg ttattgctac ttcgcccgtt
    14761 aaccctgatg gggcttgatg atggcgtggc gcgcaatctc gggctggcct tgtcgctcgc
    14821 gcgtctggca gcgctgtcgc tggcgattgt catcagtgcg ctgctggtga acgctgtggg
    14881 gattatcggc tttatcgggt tgttcgcgcc gctgctggca aaaatgctgg gggcgcggcg
    14941 tctgctgcca cgactgatgc tggcgtcgct gattggggcg ctgatcctct ggctttccga
    15001 tcaaatcatc ctctggctga ctcgcgtgtg gatggaagtg tccaccggtt cggtcactgc
    15061 gttgatcggt gcgccgctgc tactgtggct gttgccgcgt ttacgcagca ttagcgcgcc
    15121 ggatatgaag gtcaacgatc gtgtcgcggc tgaacgccaa catgtgctgg cgtttgccct
    15181 tgctggcggt gtgctgctga taattgctgt ggtggtggcg ctgtcgtttg gtcgtgatgc
    15241 gcacggctgg acgtgggcga gcggggcgct gctcgatgat ttaatgccct ggcgctggcc
    15301 gcgaattatg gcggcgctgt ttgcgggcgt catgctggcg gtggcgggct gtattattca
    15361 gcggctgacc ggaaacccga tggcaagccc ggaagtgctg gggattagct ctggcgcagc
    15421 gtttggcgtg gtgttgatgc tgtttctggt gccgggtaat gcctttggct ggttgttacc
    15481 tgcaggcagt ctcggggcgg cggtgacgct gttgatcatt atgatcgccg ccggacgcgg
    15541 tggattttcc ccacaccgca tgttactggc ggggatggcg ttaagtaccg cgttcaccat
    15601 gcttttgatg atgttgcagg caagtggtga cccgcgaatg gcgcaagtgc tgacctggat
    15661 ttccggttcg acctacaacg cgaccgatgc gcaggtctgg cgcaccggaa ttgtgatggt
    15721 gattttgctg gcgattaccc cgctgtgccg ccgctggctg accattttac cgctgggtgg
    15781 tgataccgcc cgagccgtag gaatggcgct aacgccgacg cgaattgcgc tgctgctgtt
    15841 agcggcttgc ctgacggcga ccgcgacgat gactattgga ccgttgagtt ttgttggttt
    15901 aatggcaccg catattgcgc ggatgatggg ctttcgacgg acgatgccgc acatcgtaat
    15961 ttcggcgctg gtgggtggtt tactgctggt gttcgctgac tggtgtgggc ggatggtgct
    16021 gtttccattc cagatcccgg cggggctgct gtcaaccttt atcggcgcgc catattttat
    16081 ctatttgttg agaaagcaga gccgttaatt gaatgcctcg cccggtgggc gaggcaaaat
    16141 catcacaact tcgcaaacac ccgacgtgca gcatcgatgg tgttattgat atcttccatg
    16201 ctgtgcgcca cggacataaa gcccgcttca aacgctgacg gtgccaggta aacaccttca
    16261 tccagcatca tatggaagaa acgcttaaag cgttccacgt cacaggccat cacatcctga
    16321 tagcacgtca cggactcggc gtcggtaaag aaaataccga acatgccgcc aacgtggtta
    16381 acgaccagcg gaattccggc ttcttctgcc gcttccagca gaccttctgc cagacgtgat
    16441 gtcagctcat ccaacgtttc gtgaacgccc ggctgcgcga cttcattcag acaggcgaaa
    16501 ccggctgcca tcgcaattgg gttaccggaa agcgtacccg cctgatagac cggacccgtc
    16561 ggggccagcg catccattac atcacgacga ccaccgaatg cgcctaccgg cattccaccg
    16621 ccgatgattt ttccgaggca ggttaaatct ggcactacgc cgtaataatc ctgtgcacca
    16681 gccagtgcca cgcggaagcc agtcattact tcatcgatga tcagcaatgc gccaaattcg
    16741 tcgcacagcg cgcgcagacc tggcaggaac tctggcagcg gcggaacaca gttcatattg
    16801 cctgccaccg gctcaacgat aatacaggca atctcttgcg ggtattgctc aaacgcggcg
    16861 cgtacagaag ccagatcgtt ataagtacag gttaaggtat gtttggcgaa atctgccgga
    16921 acacccggcg agtttggctg gcctaacgtg agtgcgccag aaccggcttt caccagcagg
    16981 cagtcagcgt gaccgtggta acaaccttca aatttaatga ttttgtcgcg accggtaaaa
    17041 ccacgggcca ggcggatggc gctcatggtc gcttcagtgc cggagttcac catgcgcacc
    17101 atatccatgg tcgggaccag ttcggtcacc agttgcgcca ttttcacttc catttcggtt
    17161 ggtgcaccaa agcttaaacc acgctcggcg gcttcaatca cggcattgcg gattgccgga
    17221 tggttatggc ccagcaccat cggcccccag gaaccgacat aatcgatata ggctttgcca
    17281 tcaacatcgt acagataagc gccgtccgct ttttcgataa acagtggagt gccgcccacg
    17341 ccagtaaagg cgcgaacagg ggagttcaca ccgccaggga tcagctcgcg cgctgcgctg
    17401 taaagatttt cagacttact catggagggt tcctgattcg tagaaaaagt gaatggctgc
    17461 tattctatgt tattcataac aagttaaata ctcgtcaaac atcaggctgc ttgtactggt
    17521 gcaatcctga tttcttagag tataaaagtt ttgtgcattt gaaacattac gctttgcaaa
    17581 ggattttcat ggaacgtgcg agtaaaatgc cgtcatctta tttgtatgac caataagtga
    17641 tcattggatg aaaactgata ctccctcttt agaaacaccg caggccgcgc gcctgcgacg
    17701 cagacaactg attcgccaac ttcttgagcg cgataaaacc ccgttagcca ttttgtttat
    17761 ggcggcagtc gtcggcacgc ttgtcgggct ggctgcggtt gcttttgaca aaggtgtcgc
    17821 ctggttgcag aaccaacgta tgggggcgct ggtacatact gctgataatt atccgctact
    17881 gttaactgtc gcttttctct gttcggcggt gctggcgatg ttcggctact ttttggtgcg
    17941 caaatacgcg cccgaagcag ggggatcggg gatcccggaa attgaagggg cgctggaaga
    18001 tcaacgtcct gttcgctggt ggcgtgtatt gccggtgaaa ttctttggcg ggctggggac
    18061 actcggcgga ggcatggtgc tagggcgcga aggaccaacc gtgcagatcg gcggcaatat
    18121 cggtcgcatg gtgcttgata ttttccgcct gaaaggtgat gaagctcgcc atacgctgct
    18181 ggcaaccggt gctgctgcgg ggctggctgc ggcctttaac gcgccgctgg cgggtatttt
    18241 gtttattatc gaagagatgc gtccgcagtt tcgctatacg ttaatttcga ttaaagcggt
    18301 atttattggc gtcattatgt cgaccattat gtaccggatt tttaatcatg aagttgcgtt
    18361 gattgacgtc ggtaaacttt ctgatgcgcc gcttaatacg ctgtggcttt atctgatcct
    18421 cggtattatt tttggcattt tcggccctat ttttaataaa tgggtgctgg ggatgcagga
    18481 tttgctgcac cgtgtgcacg gcggcaatat taccaaatgg gtgctaatgg gcggtgcgat
    18541 tggcggtctg tgtggattgc tggggtttgt ggcaccagca acgtcgggcg gcggttttaa
    18601 cctgattcct atcgctaccg cggggaattt cagcatggga atgctggtgt ttatcttcgt
    18661 cgcgcgggtc attaccacct tactctgctt ctcttccggc gcgccgggcg gtatttttgc
    18721 cccgatgctg gcgctgggta ctgtgctggg aaccgctttc ggaatggttg ccgttgagct
    18781 gtttccgcaa tatcaccttg aggcgggaac gtttgctatt gccggaatgg gggcgttact
    18841 ggcggcatct attcgcgcgc cgttaacggg gatcattctg gttctggaga tgaccgataa
    18901 ctaccagctc attttgccaa tgattattac cggtcttggc gcaacactat tagcgcaatt
    18961 taccggcggg aaaccgctat actcggcgat tcttgcgcgc acgctggcaa aacaggaagc
    19021 tgagcaactg gcgcgaagca aggccgcatc agccagcgag aatacttgaa cgaaatacca
    19081 gggtattaga taatggcgat tattattggg ttagaatttg cccaattgcc gatgtcgttt
    19141 ggagcaaaat atgagtgatg acgtagcact gccgctggag tttaccgacg cagcagccaa
    19201 caaagttaaa agcctgatcg ctgacgaaga taacccgaat ctgaaattac gcgtgtatat
    19261 caccggtggc ggttgcagcg gcttccagta tggtttcacc tttgatgatc aggtgaacga
    19321 aggcgatatg accatcgaaa aacagggcgt tggcctggtg gttgatccga tgagcctgca
    19381 atatctggtc ggcggttccg ttgactatac cgaaggactg gaaggttctc gtttcatcgt
    19441 gaccaacccg aacgcgaaaa gcacctgcgg ttgcggttct tcctttagca tctaatctgt
    19501 tgttcatgtg ccggataaac tatccggcac atcttctctc ttcaacgccc attctcatcc
    19561 agcgcaaacg tcggtagctt aagatgccaa cgaatagccg ccagccgaat caatagcgtc
    19621 acgaccatgc ccatcatact ggctgtttcc agtggtacgg aaaatgtgta ataagccgta
    19681 gcgtggacaa taccgccgat aatacaggca gttgcgtaga tttctgtacg taaaatcatg
    19741 gggatctcgc gggccagtac atcacgaata atcccgccgc caacgccagt aatgacgccc
    19801 atacaaaccg cgattaacgg accggcttcc gcattaaagg ctttattcac gccaatgccg
    19861 acaaacaccg ccagaccaac ggcgtccagc accggcaaca tccattttgg taagcgtctt
    19921 ggctggcgca ccagcacgat ggtcagcatg ctggtgacca ttgcaacgac cagatcggtg
    19981 ggatctttca cccaaaatac cgggccgtga tccagcgcca tgtcgcgaat tgttccgccg
    20041 cctactgcgg ttaccacgcc cagtaccaga acaccaaaag ggtccatacg caattttccg
    20101 gctaacaaaa cgccggagat ggcaaatacc gctgtgccga ctatatccag ccaatagacg
    20161 agcattgttc aatccccact gagcacctga aaaggtcagg cactaatcta cctgtgaaag
    20221 cgcattacag agctgttgtg ctgcgaggat aatacgtggg cttgcacgtt caaaccagtc
    20281 actcgtgaga ggaataacgg gaattttgag ctgttcaccc cagtattgtt tgattttagg
    20341 aatttggtcc ggtccgcctg taatgacaat cgcctgtggc gagcgtgcta acacctgttc
    20401 gcggctaact tgcggccagg gaacccggct gtctttaaag atgttttctc cgccacaaac
    20461 ttcgagtacc tggttctgaa tcgactcttt tccactggta aatggcggat taatgccgaa
    20521 ttgcagaaaa acacgttttt taggtttatc agcatattgc gctttcaatt gcgcgtactg
    20581 atccagcagg gattgcgcgg cttgttcggc cttgtctggt tgcggactcc agggggccag
    20641 ttgacgtaac gcattggcaa tttgttcaat gcttgtcgca tcgacccaca tcacttttat
    20701 tcccagcgaa gccagctggt caacctgccg ctcggcatta cctccacgcc aggcaatcac
    20761 cagatcgggc ttcagcgcga caatgcgttc cagattcatc ccctgccagg tggaaacctg
    20821 ctcaatcttt tgcgcttgtg gaggatagtc ggaatagctg ctgaccccaa ccggcgtgat
    20881 cccggcagca aaggcaagtt cagtgttggc gggagaaagc gtgatgacgc gcggcgcggc
    20941 gttgagccac agtggcgcaa gaaaagacag ggcgaccagc gccctgaaca gtgacttagc
    21001 catgtgcaag tttctgcacc agtgactcaa ccatcaggct ggactgttta gcggcaacag
    21061 ccaggaactc atcgaagcta agatgagact gttgatcggc cacgtcggag atggcgcgta
    21121 cgacaacaaa cgggacgttg aaattgtggc agacatgggc gattgccgtc gcttccatct
    21181 ctacagcaat ggcctgtggg aagttgtggc ggattttcgc cagaccaaca gaaccgttga
    21241 tgaaagcgtc gccgctaaca atcaggccac gtacagcgtt aagattcagt tcggcaatgc
    21301 aggcctcagc ggcagcgatc agtttatcgt cagctttaaa gcctgccgga cagcctggta
    21361 actgaccgta ttcataacca aatgccgtga catccgcgtc gtgataacgt gcttcgtccg
    21421 agacaacgat atcgcccact ttcaacgttg gtgccaggcc accggcagaa ccggtgttaa
    21481 taatcacatc tggcttgcag tgttccaaca gcaaagtggc acccagcgcc gcagcgactt
    21541 taccgatgcc cgatttcaga agcgcaacct cggttccatt cagttggccg gtatagattt
    21601 cgcaaccgcc gagactgata gtttgacgtt tttcgatttt gtcacgcagc agcgtaactt
    21661 cttcttccat tgcaccaatg atgccgattt tcatagattt actcgcgata agcccgattt
    21721 gaaggcatag tttaccatgc gcttacgggg aagcgtattt ctcacgcggg agaggacatg
    21781 gcacagattg atttccgaaa aaaaataaac tggcatcgtc gttaccgttc accgcagggc
    21841 gttaaaaccg aacatgagat cctgcggatc ttcgagagcg atcgcgggcg tatcatcaac
    21901 tctccggcaa ttcgtcgtct gcaacaaaag acccaggttt ttccactgga gcgcaatgcc
    21961 gccgtgcgca cgcgtcttac ccactcgatg gaagtccagc aggtggggcg ctacatcgcc
    22021 aaagaaattc taagccgtct gaaagagctt aaattactgg aagcatacgg cctggatgaa
    22081 ctgaccggcc cctttgaaag cattgttgag atgtcatgcc tgatgcacga tatcggcaat
    22141 ccgccgtttg gtcattttgg cgaagcggcg ataaatgact ggtttcgcca gcgtttatac
    22201 ccggaagatg ccgaaagcca gcctctgact gacgatcgct gcagcgtggc agcactacgt
    22261 ttacgggacg gggaagaacc gcttaacgag ctgcggcgca agattcgtca ggacttatgt
    22321 tattttgagg ggaatgcaca aggcattcgt ctggtgcata cattgatgcg gatgaatctc
    22381 acctgggcac aggttggcgg tattttaaaa tatacccgtc cggcgtggtg gcgtggcgaa
    22441 acgcctgaga cacatcacta tttaatgaaa aagccgggtt attatctttc tgaagaagcc
    22501 tatattgccc ggttgcgtaa agaacttaat ttggcgcttt acagtcgttt tccattaacg
    22561 tggattatgg aagctgccga cgatatctcc tattgtgtgg cagaccttga agatgcagta
    22621 gagaaaagaa tatttaccgt tgagcagctt tatcatcatt tgcacgaagc gtggggccag
    22681 catgagaaag gttcgctctt ttcgctggtg gttgaaaatg cctgggaaaa atcacgctca
    22741 aatagtttaa gccgcagtac ggaagatcag ttttttatgt atttacgggt aaacacccta
    22801 aataaactgg taccctacgc ggcacaacga tttattgata atctgcctgc gattttcgcc
    22861 ggaacgttta atcatgcatt attggaagat gccagcgaat gcagcgatct tcttaagcta
    22921 tataaaaatg tcgctgtaaa acatgtgttt agccatccag atgtcgagca gcttgaattg
    22981 cagggctatc gggtcattag cggattatta gagatttatc gtcctttatt aagcctgtcg
    23041 ttatcagact ttactgaact ggtagaaaaa gaacgggtga aacgtttccc tattgaatcg
    23101 cgcttattcc acaaactctc gacgcgccat cggctggcct atgtcgaggc tgtcagtaaa
    23161 ttaccgtcag attctcctga gtttccgcta tgggaatatt attaccgttg ccgcctgttg
    23221 caggattata tcagcggtat gaccgacctc tatgcgtggg atgaataccg acgtctgatg
    23281 gccgtagaac aataaccagg cttttgtaaa gacgaacaat aaatttttac cttttgcaga
    23341 aactttagtt cggaacttcg cgctataaaa cgaatctgaa gaacacagca attttgcgtt
    23401 atctgttaat cgagactgaa atacatgaaa aaaaccacat tagcactgag tgcactggct
    23461 ctgagtttag gtttggcgtt atctccgctc tctgcaacgg cggctgagac ttcttcagca
    23521 acgacagccc agcagatgcc aagccttgca ccgatgctcg aaaaggtgat gccttcagtg
    23581 gtcagcatta acgtagaagg tagcacaacc gttaatacgc cgcgtatgcc gcgtaatttc
    23641 cagcagttct tcggtgatga ttctccgttc tgccaggaag gttcaccgtt ccagagctct
    23701 ccgttctgcc aggggggcca gggcggtaat ggcggcggcc agcaacagaa attcatggcg
    23761 ctgggttccg gcgtcatcat tgatgccgat aaaggctatg tcgtcaccaa caaccacgtt
    23821 gttgataacg cgacggtgat taaagtccag ctgagcgatg gtcgtaagtt cgacgcgaag
    23881 atggttggca aagatccgcg ctctgatatc gcgctgattc agatccagaa cccgaaaaac
    23941 ctgaccgcaa ttaagatggc ggattctgat gcactgcgcg tgggtgatta caccgtagcg
    24001 attggtaacc cgtttggcct gggagagacg gtaacttccg ggattgtctc tgcgctgggg
    24061 cgtagcggcc tgaatgccga aaactacgaa aacttcatcc agaccgatgc agcgatcaac
    24121 cgtggtaact ccggtggtgc gctggttaac ctgaacggcg aactgatcgg tatcaacacc
    24181 gcgatcctcg caccggacgg cggcaacatc ggtatcggtt ttgctatccc gagcaacatg
    24241 gtgaaaaacc tgacctcgca gatggtggaa tacggccagg tgaaacgcgg tgagctgggt
    24301 attatgggga ctgagctgaa ttccgaactg gcgaaagcga tgaaagttga cgcccagcgc
    24361 ggtgctttcg taagccaggt tctgcctaat tcctccgctg caaaagcggg cattaaagcg
    24421 ggtgatgtga tcacctcact gaacggtaag ccaatcagca gctttgccgc actgcgtgct
    24481 caggtgggta ctatgccggt gggcagcaaa ctgaccctgg gcttgctgcg cgacggtaag
    24541 caggtcaacg tgaatctgga actgcagcag agcagccaga atcaggttga ttccagctcc
    24601 atcttcaacg gcattgaagg tgctgagatg agcaacaaag gcaaagatca gggcgtggta
    24661 gtgaacaacg tgaaaacggg cactccggct gcgcagatcg gcctgaagaa aggtgatgtg
    24721 attattggcg cgaaccagca ggcagtgaaa aacatcgctg aactgcgtaa agttctcgac
    24781 agcaaaccgt ctgtgctggc actgaacatt cagcgcggcg acagctccat ctacctgtta
    24841 atgcagtaat ctccctcaac cccttcctga aaacgggaag gggttctcct tacaatctgt
    24901 gaacttcacc acaactccat acatcttcat catcctttag gcatttgcac aatgccgtac
    24961 gttacgtact tccttatgct aagccgtgca taacggagga cttatggctg gctggcatct
    25021 tgataccaaa atggcgcagg atatcgtggc acgtaccatg cgcatcatcg ataccaatat
    25081 caacgtaatg gatgcccgtg ggcgaattat cggcagcggc gatcgtgagc gtattggtga
    25141 attgcacgaa ggtgcattgc tggtactttc acagggacga gtcgtcgata tcgatgacgc
    25201 ggtggcacgt catctgcacg gtgtgcggca agggattaat ctaccgttac ggctggaagg
    25261 tgaaattgtc ggcgtaattg gcctgacagg tgaaccagag aatctgcgta aatatggcga
    25321 actggtctgc atgacggctg aaatgatgct ggaacagtcg cggttgatgc acttgttggc
    25381 tcaggatagc cgtttgcggg aggaactggt gatgaacctg attcaggcag aggagaatac
    25441 tcccgcactt actgaatggg cgcaacggct ggggatcgat ctcaatcaac cgcgagtggt
    25501 ggctattgtt gaggtcgaca gcggtcagct tggtgtggac agcgcaatgg cggagttaca
    25561 acaactgcaa aacgcgctga ctacgcccga gcgtaataat ctggtggcga ttgtctcgct
    25621 aaccgaaatg gtggtgttga aaccggcgtt gaactctttt gggcgctggg atgcagaaga
    25681 tcatcgtaag cgagttgaac aactgattac ccgcatgaaa gagtacggcc agctgcgttt
    25741 tcgcgtttca ctgggcaact attttaccgg tcctggcagt attgcccgat cctatcgtac
    25801 ggcgaaaacg acgatggtgg tgggtaaaca gcggatgcca gaaagtcgct gctattttta
    25861 tcaggatctg atgttacctg tgttactcga cagtttgcgt ggcgactggc aggccaacga
    25921 actggcgcga ccgctggcgc ggctgaaagc gatggacaat aacggcttgc tgcgacgaac
    25981 gctggcggcg tggtttcgtc acaatgtgca accgctggca acgtcaaagg cgttgtttat
    26041 tcatcgtaat actctggagt atcggcttaa tcgtatatcg gaactgaccg ggcttgattt
    26101 gggtaatttt gatgacaggt tgctgctgta tgtggcgttg cagctggatg aagagcggta
    26161 ggttatgcgt taaggtcgag gcggcgctgg ctcattcccc tcagcccgaa gggtagaggg
    26221 gaatgtccga gttaaccgtc agttcggcat tatttacgcg ttagtttttc cagatcggct
    26281 tcaatctcgc tgatcttatt ggttacgact gactccaggt gacgtaagtc gtcgaggatc
    26341 ttacgcttaa gatcaacttc gctgcggtca cgctggcaga tttgatcaag ctcatcaatg
    26401 atataccgta gattcgggct gatttcctgg acttctttat aaccctgacc cacaccatca
    26461 gcgacgaccg ttttacgctg acgcggatat ttaaacttaa cgctcttggc gaaaaactcg
    26521 cctttgtctt tctggaaata gattttcaga atatcgttgt tggcttcctg ccggaggctg
    26581 taacgatcaa tttcttcagg attggtaata cccagacttt tcagattgtc gtacatagcg
    26641 ttacctcaaa atgagtcagt aaattgtgct tattttagca tttggcctgg cccgcccggc
    26701 tcgactgtga tcgcagaaag cctttttctt ccagacttct atcatggcgt aaaaatcaaa
    26761 aaattacctg ctttattgtg gcgatattcc atgggatgga ttaacacatt tatcttcgtt
    26821 ttgcgaatta catgacagtc cgaaacaaga tgaaaaagta tttaaaaaag gggggaagaa
    26881 gaatgagtag gacgaattat tctgagccag aaagcatacg ggcagcatca tgctgcccgt
    26941 atatgtactc ttaatcgatg gtacgcagca gttcgttaat gccgactttg ccgcgagttt
    27001 tcgcgtcaac tttcttaacg ataaccgcac agtagaggct gtatttgcca tctttcgacg
    27061 gcagattacc tgaaacaacc acagaccccg ccggaacgcg accgtagtgg atttcgccgg
    27121 tttcacggtc gtaaatacgg gtgctctgac caatgtatac gcccatagag atgacggaac
    27181 cttcttcgac aatcacccct tcaaccactt cggagcgtgc gccaatgaag cagttatctt
    27241 caataatggt cgggttagcc tgcagcggct ccagcacgcc gccgatgccc acgccaccgg
    27301 aaaggtggac gtttttacca atctgcgcac aagaaccgac ggtcgcccag gtatcaacca
    27361 tcgtgccttc atcaacatat gcgccgatgt tgacgtaaga cggcatcagc acggtgttac
    27421 gggcaataaa tgcaccctga cgtaccgccg ctggtggcac aacgcggaag ccttctttct
    27481 gaaaacgcgc ttcgtcgtag tcggcgaatt tcatcggcac tttatcgaag tagcggcttt
    27541 ctgccccttc gatcacctga ttatcattaa tacggaaaga gagcagcacc gcttttttca
    27601 accactgatg cgtcacccac tgaccgtcaa ttttttccgc tacacgcagt gcgccggaat
    27661 ccagcagggc gatcacctga tttaccgctt cgcgggttac ggtgtctgca ttggctggcg
    27721 tgatctcggc acggcgttca aaagcggttt caataatgtt ctgtaactgc tgcattgtta
    27781 aactcttttc atatcagtaa acacatcacc ctttatcgtt tggattgagg gcctctgtca
    27841 accgctgatg cacttcctgc tgcaactcgt tattaagtgc acgccggtca gcggtggcaa
    27901 ttatgaataa atcttctact cgctcgccaa tggttgtaat tcgggcacca tgaagcgaaa
    27961 ttcccagatc ggcaaaaatt ttcccgactc tcgccagcag tcctggctgg tcgagcgcga
    28021 taagctcaag gaacgatttg cggtcggtat gggtcggcaa aaacgttact tcggtttcaa
    28081 cagtaaaatg gcgtaatttg gcgggttggc gacggggctg cggtggctgc cagctacttt
    28141 gcgtcagtac ttgctccaga ccaaaccgaa taacctcatg acgatctgcg gacagcgggc
    28201 tgccatcggg ttccagcacg ataaaggtat ccatcgccat accgtcgcga gtggtgaaaa
    28261 tttgtgcgtc gtgaacactt aaattgcggc ggtctaattc ggcacagacg gcggcaaaca
    28321 gataggggcg gtccgggctc cagataaaaa tctcggtgcc tccacgcgta gcctgcgggc
    28381 taagcaatac cagcggtttg cttaaatcat gctgtaataa atggcgggca tgccaggcca
    28441 gttgatttgg gctatggcgg acaaaatagt tagcacgaca gcgtgaccaa atttggtgca
    28501 gcgcctcttc gtcgatgtta tccatgcgca gtagtgccag tgcctggagt tggtgatggc
    28561 gaacccgttc gcgcatatcc ggcgtgtttt gcattccgcg tcgtagctgc ttttcggtgg
    28621 caaaatagag ttcgcgcaac agactttgct tccagctatt ccacagcgtt tcgttggtgg
    28681 cgcaaatgtc agccactgtc aggcatacca gatagcgcag acgattttcc gtttgaactt
    28741 cttcggcaaa ctgcttgatg acttcagggt cctgaatatc gcggcgttgt gctgtcaccg
    28801 acatcagcaa gtgctggcga accagccagg caaccagctg tgtttcgcgt gagttcagcc
    28861 cgtggagttc tgcaaaatgc actacatcct gagcaccgag aatggagtgg tcgccgccgc
    28921 gtcctttggc gatatcgtga aacagcgcgg cgatgaaaat cagctcagtt gacggcaggc
    28981 gcggccagac gtccacacac aacggatggc gctggcgcgt ttcttcactg gcaaaactct
    29041 ccagtttcag catcacgcgg atagtatgtt catccaccgt gtaggcgtgg aacagatcaa
    29101 actgcatctg cccgacgata tgcgaccatt gcggcatata cgcgccgagc acgctatggc
    29161 gatgcattgg caatagcccg cgccgcaccg ctccggggtg acgcagaatg ctcaaaaaca
    29221 gttttcgtgc ttccggaata ttacacagcg gttgttgcag atggcgacgg gcatggcgta
    29281 actggcgcag cgtggtggag taaatgccgg tgatcgcact gttgcgcacc atggtgtaga
    29341 acatacgcaa gatggcttcc ggctggcgca taaatagtgt ttcatcacgc aggtcgatta
    29401 gcgtaccgcg tagctgaaac tcatcgtcga ttggacgtgg tttttcgtcg gcgggaaggg
    29461 cgaggatggc ttcatcgaac agttgcagca gcatctggtt gagttcactg acgcggcgtg
    29521 taacgcggaa gtaatccttc atcatccgct cgaccggttc gttgccttca ccactgtaat
    29581 tcagacgctg agcgacgcta agttggcgat cgaataacag acgattatcg taacggctga
    29641 cgaccagatg cagggcaaag cgaatacgcc acagtatatg cagacattcg tttaattccg
    29701 cccgctccgc tgaggttaag aagccaaacc cgaccatttc atccagcgat gttgcgccaa
    29761 aatgacggcg ggccacccat tgcagagtgt gaatatcgcg caagccgcca gggctacttt
    29821 tgatgtctgg ttcaaggttg tagctggtgc catggtaacg ctgatggcgc tggttctgtt
    29881 cttcaacttt cgccgcgtag aacttgtcgg aaggccagaa tccttcgcta aaaatatgtt
    29941 tttgcagttc gaggaagagc gcaacatcgc caattaataa gcgggattcg attaaattgg
    30001 tggcgacggt taaatccgat aacccttcca gcatgcactc ttcaagcgtg cgcacactat
    30061 gaccgacttc cagctttaca tcccagagca gcgttaacag ctcgcccact ttttgcgcct
    30121 gatcgtccgg gagcttttta cggcttaaaa tcagtaaatc gatgtcagaa agtggatgca
    30181 gctcgccacg accgtagcca ccgacggcga ccaatgccag gtcggcaatc tggctgaatc
    30241 ccgcttcaat ccataatcgt tgcaggagct ggtcgataaa ctcggtgcgc gcctcaatca
    30301 actgttctgc agagatccca ttgtcaaagg catcacccag ccaacgctgg aaagtatcga
    30361 tatgggcttt tatcccaccg acggttaatt catcacgggg ccagacgcat ggattttgcg
    30421 gttgaccggg cagggtgggg agagcggtgt ttgcgtactg ttctggaagg gtattcattg
    30481 tgcgccaccc ataagattaa attatcgcat taaaaaagcc ggcattcgcc ggcttcatct
    30541 tattcgtcgt gcgagattat cgccgggatg gtgtcatcct tgcgtagcgt caaaatttcg
    30601 cagccgttat cagtcaccac aatagtatgc tcatattgtg cagacaagct gcgatctttg
    30661 gtttttaccg tccagccatc tttcatggtg cggatctctt ttttacccgc gttgaccatt
    30721 ggctcaatgg tgaacgtcat ccccggtttc agcacgacgt tggtttcacg ggagtcatag
    30781 tgcagcacct gcggttcttc atggaagccg cgaccaatac cgtgtccaca atattcacga
    30841 acgacggaga agccttctgc ttcgacaaac ttctgaatcg ccgcaccgat ttcgcgcagg
    30901 ttaatgcctg gtttgaccat gcgcagcgcc aggtacaggc tttcttgcgt gatgcggcac
    30961 agacgttcgc ccatgatggt cggcttaccg acgataaaca ttttcgaggt atcgccgtgg
    31021 aaaccatctt tgattacggt gacatcaatg ttaacgatat cgccatcttt cagcagctta
    31081 gcatcgtccg ggataccgtg gcacaccact tcattaatag agatgcaaac ggatttcgga
    31141 tagccgtgat agccgaggca ggcagaaacc gcgtgttgtt cattaacaat gtaatcatta
    31201 cagatgcgat ccagctcgcc ggtgctgacg cccggtttaa catacggttc gatcatctcc
    31261 agcacttcgg cagccagtcg gccagcgacg cgcatttttt cgatatcttc tggggtcttg
    31321 attgagatag ccattaattc tgtccatcag cgtcggtgat accgacaata tatatgtaag
    31381 tgccgtcaat ggtatcacac ccgggcaaat tgagaatcat tctgaatttc gccaaacgtg
    31441 ccactgaagg ttttctataa tagaaaattc gacatctgat gctgtacaca gcgccaacaa
    31501 ttattggtgt ccatgacgta tttgtggtat aaagcgcgcc ggacttccga tccatttcgt
    31561 atacacagac tggacggaag cgacaatctc actttgtgta acaacacaca cgtatcggca
    31621 catattccgg ggtgcccttt ggggtcggta atatgggata cgtggaggca taaccccaac
    31681 ttttatatag aggttttaat catggcaact gtttccatgc gcgacatgct caaggctggt
    31741 gttcacttcg gtcaccagac ccgttactgg aacccgaaaa tgaagccgtt catcttcggt
    31801 gcgcgtaaca aagttcacat catcaacctt gagaaaactg taccgatgtt caacgaagct
    31861 ctggctgaac tgaacaagat tgcttctcgc aaaggtaaaa tccttttcgt tggtactaaa
    31921 cgcgctgcaa gcgaagcggt gaaagacgct gctctgagct gcgaccagtt cttcgtgaac
    31981 catcgctggc tgggcggtat gctgactaac tggaaaaccg ttcgtcagtc catcaaacgt
    32041 ctgaaagacc tggaaactca gtctcaggac ggtactttcg aaaagctgac caagaaagaa
    32101 gcgctgatgc gcactcgtga gctggagaaa ctggaaaaca gcctgggcgg tatcaaagac
    32161 atgggcggtc tgccggacgc tctgtttgta atcgatgctg accacgaaca cattgctatc
    32221 aaagaagcaa acaacctggg tattccggta tttgctatcg ttgataccaa ctctgatccg
    32281 gacggtgttg acttcgttat cccgggtaac gacgacgcaa tccgtgctgt gaccctgtac
    32341 ctgggcgctg ttgctgcaac cgtacgtgaa ggccgttctc aggatctggc ttcccaggcg
    32401 gaagaaagct tcgtagaagc tgagtaataa ggcttgataa ctcccccaaa atagttcgag
    32461 ttgcagaaag gcggcaagct cgagaattcc cgggagctta catcagtaag tgaccgggat
    32521 gagcgagcga agataacgca tctgcggcgc gaaatatgaa gggggagagc ccttatagac
    32581 caggtagtac acgtttggtt agggggcctg catatggccc cctttttcac ttttatatct
    32641 gtgcggttta atgccgggca gatcacatct ccgaggattt tagaatggct gaaattaccg
    32701 catccctggt aaaagagctg cgtgagcgta ctggcgcagg catgatggat tgcaaaaaag
    32761 cactgactga agctaacggc gacatcgagc tggcaatcga aaacatgcgt aagtccggtg
    32821 ctattaaagc agcgaaaaaa gcaggcaacg ttgctgctga cggcgtgatc aaaaccaaaa
    32881 tcgacggcaa ctacggcatc attctggaag ttaactgcca gactgacttc gttgcaaaag
    32941 acgctggttt ccaggcgttc gcagacaaag ttctggacgc agctgttgct ggcaaaatca
    33001 ctgacgttga agttctgaaa gcacagttcg aagaagaacg tgttgcgctg gtagcgaaaa
    33061 ttggtgaaaa catcaacatt cgccgcgttg ctgcgctgga aggcgacgtt ctgggttctt
    33121 atcagcacgg tgcgcgtatc ggcgttctgg ttgctgctaa aggcgctgac gaagagctgg
    33181 ttaaacacat cgctatgcac gttgctgcaa gcaagccaga attcatcaaa ccagaagacg
    33241 tatccgctga agtggtagaa aaagaatacc aggtacagct ggatatcgcg atgcagtctg
    33301 gtaagccgaa agaaatcgca gagaaaatgg ttgaaggccg catgaagaaa ttcaccggcg
    33361 aagtttctct gaccggtcag ccgttcgtta tggaaccaag caaaactgtt ggtcagctgc
    33421 tgaaagagca taacgctgaa gtgactggct tcatccgctt cgaagtgggt gaaggcatcg
    33481 agaaagttga gactgacttt gcagcagaag ttgctgcgat gtccaagcag tcttaattat
    33541 caaaaaggag ccgcctgagg gcggcttctt tttgtgccca tcttgtaaat tcagctaacc
    33601 cttgtggggc tgcgctgaaa agcgacgtac aatgtcgcta gtattaattc atttcaatcg
    33661 ttgacagtct caggaaagaa acatggctac caatgcaaaa cccgtctata aacgcattct
    33721 gcttaagttg agtggcgaag ctctgcaggg cactgaaggc ttcggtattg atgcaagcat
    33781 actggatcgt atggctcagg aaatcaaaga actggttgaa ctgggtattc aggttggtgt
    33841 ggtgattggt gggggtaacc tgttccgtgg cgctggtctg gcgaaagcgg gtatgaaccg
    33901 cgttgtgggc gaccacatgg ggatgctggc gaccgtaatg aacggcctgg caatgcgtga
    33961 tgcactgcac cgcgcctatg tgaacgctcg tctgatgtcc gctattccat tgaatggcgt
    34021 gtgcgacagc tacagctggg cagaagctat cagcctgttg cgcaacaacc gtgtggtgat
    34081 cctctccgcc ggtacaggta acccgttctt taccaccgac tcagcagctt gcctgcgtgg
    34141 tatcgaaatt gaagccgatg tggtgctgaa agcaaccaaa gttgacggcg tgtttactgc
    34201 tgatccggcg aaagatccaa ctgcaaccat gtacgagcaa ctgacttaca gcgaagtgct
    34261 ggaaaaagag ctgaaagtca tggacctggc ggccttcacg ctggctcgtg accataaatt
    34321 accgattcgt gttttcaaca tgaacaaacc gggtgcgctg cgccgtgtgg taatgggtga
    34381 aaaagaaggg actttaatca cggaataatt cccgtgatgg ataaataagg gtaagattcc
    34441 gcgtaagtat cgcgggggat cttacccgta atatgtttaa tcagggctat acttagcaca
    34501 cttccactgt gtgtgactgt ctggtctgac tgagacaagt tttcaaggat tcgtaacgtg
    34561 attagcgata tcagaaaaga tgctgaagta cgcatggaca aatgcgtaga agcgttcaaa
    34621 acccaaatca gcaaaatacg cacgggtcgt gcttctccca gcctgctgga tggcattgtc
    34681 gtggaatatt acggcacgcc gacgccgctg cgtcagctgg caagcgtaac ggtagaagat
    34741 tcccgtactc tgaaaatcaa cgtgtttgat cgttcaatgt ctccggccgt tgaaaaagcg
    34801 attatggcgt ccgatcttgg cctgaacccg aactctgcgg gtagcgacat ccgtgttccg
    34861 ctgccgccgc tgacggaaga acgtcgtaaa gatctgacca aaatcgttcg tggtgaagca
    34921 gaacaagcgc gtgttgcagt acgtaacgtg cgtcgtgacg cgaacgacaa agtgaaagca
    34981 ctgttgaaag ataaagagat cagcgaagac gacgatcgcc gttctcagga cgatgtacag
    35041 aaactgactg atgctgcaat caagaaaatt gaagcggcgc tggcagacaa agaagcagaa
    35101 ctgatgcagt tctgatttct tgaacgacaa aaacgccgct cagtagatcc ttgcggatcg
    35161 gctggcggcg ttttgctttt tattctgtct caactctgga tgtttcatga agcaactcac
    35221 cattctgggc tcgaccggct cgattggttg cagcacgctg gacgtggtgc gccataatcc
    35281 cgaacacttc cgcgtagttg cgctggtggc aggcaaaaat gtcactcgca tggtagaaca
    35341 gtgcctggaa ttctctcccc gctatgccgt aatggacgat gaagcgagtg cgaaacttct
    35401 taaaacgatg ctacagcaac agggtagctg caccgaagtc ttaagtgggc aacaagccgc
    35461 ttgcgatatg gcagcgcttg aggatgttga tcaggtgatg gcagccattg ttggcgctgc
    35521 tgggctgtta cctacgcttg ctgcgatccg cgcgggtaaa accattttgc tggccaataa
    35581 agaatcactg gttacctgcg gacgtctgtt tatggacgcc gtaaagcaga gcaaagcgca
    35641 attgttacca gtcgatagcg aacataacgc catttttcag agtttaccgc aacctatcca
    35701 gcataatctg ggatacgctg accttgagca aaatggcgtg gtgtccattt tacttaccgg
    35761 gtctggtggc cctttccgtg agacgccatt gcgcgatttg gcaacaatga cgcccgatca
    35821 agcctgccgt catccgaact ggtcgatggg acgtaaaatt tctgtcgatt cggctaccat
    35881 gatgaacaaa ggtctggaat acattgaagc gcgttggctg tttaacgcca gcgccagcca
    35941 gatggaggtg ctgattcacc cgcagtcagt gattcactca atggtgcgct atcaggacgg
    36001 cagtgttctg gcgcagctgg gggaaccgga tatgcgtacg ccaattgccc acaccatggc
    36061 atggccgaat cgcgtgaact ctggcgtgaa gccgctcgat ttttgcaaac taagtgcgtt
    36121 gacatttgcc gcaccggatt atgatcgtta tccatgcctg aaactggcga tggaggcgtt
    36181 cgaacaaggc caggcagcga ctacagcatt gaatgccgca aacgaaatca ccgttgctgc
    36241 ttttcttgcg caacaaatcc gctttacgga tatcgctgcg ttgaatttat ccgtactgga
    36301 aaaaatggat atgcgcgaac cacaatgtgt ggacgatgtg ttatctgttg atgcgaacgc
    36361 gcgtgaagtc gccagaaaag aggtgatgcg tctcgcaagc tgaggataat ccggctacag
    36421 agagtcgcgc tatttgttag cgtagggctt cagtgatata gtctgcgcca tctgatcgta
    36481 agtagttggc tttataaggt caggtatgcc gtggttttac acggcttttt tttgtatagg
    36541 cttcagtatt cctgagtacc gtaaaccctg tcagggaata aaaaacgcgt gatgttgtct
    36601 gctactcaac cacttagcga aaaattgcca gcgcatggct gccgtcatgt tgcgatcatt
    36661 atggacggca atggtcgctg ggcaaaaaag caagggaaga ttcgtgcctt tgggcataaa
    36721 gccggggcaa aatccgtccg ccgggctgtc tcttttgcgg ccaacaacgg tattgaggcg
    36781 ttaacgctgt atgcctttag tagtgaaaac tggaaccgac cagcgcagga agtcagtgcg
    36841 ttaatggaac tgtttgtgtg ggcgctcgat agcgaagtaa aaagtctgca ccgacataac
    36901 gtgcgtctgc gtattattgg cgataccagt cgctttaact cgcgtttgca agaacgtatt
    36961 cgtaaatctg aagcgctaac agccgggaat accggtctga cgctgaatat tgcggcgaac
    37021 tacggtggac gttgggatat agtccaggga gtcaggcaac tggctgaaaa ggtgcagcaa
    37081 ggaaacctgc aaccagatca gatagatgaa gagatgctaa accagcatgt ctgtatgcat
    37141 gaactggccc ctgtagattt agtaattagg actggggggg agcatcgcat tagtaacttt
    37201 ttgctttggc aaattgccta tgccgaactt tactttacag atgttctctg gcccgatttc
    37261 gatgaacaag actttgaagg ggcgttaaat gcctttgcta atcgagagcg tcgtttcggc
    37321 ggcaccgagc ccggtgatga aacagcctga tgggggtcgc ttttgctgaa gtatcgcctg
    37381 atatctgctt ttgtgttaat acccgtcgtc atcgcggcgt tgtttctgtt gccgccggtg
    37441 gggttcgcca ttgtaacgct ggtggtctgc atgctggcag cgtgggaatg gggacagctt
    37501 agcggtttta ccactcgttc gcagcgagta tggttggcgg tgttatgcgg gttattgttg
    37561 gcgctgatgc tttttctgtt gccggaatat caccgaaata ttcatcaacc gctggttgaa
    37621 atctcacttt gggcttcgct gggttggtgg attgtcgcgc tattgctggt gctgttttac
    37681 ccaggttccg cagcaatctg gcgtaactct aaaacattgc gccttatttt tggcgtgcta
    37741 accattgttc ccttcttctg gggcatgctg gcgttacggg cctggcacta tgacgagaat
    37801 cattacagtg gcgcaatatg gctgctctat gtcatgatcc tggtatgggg cgctgactcc
    37861 ggagcatata tgtttggcaa attgtttggt aaacataagc tggcaccgaa ggtttctccg
    37921 ggtaaaacct ggcaaggctt tatcggtgga ctcgctactg cagcggtaat ctcatggggt
    37981 tatggcatgt gggcgaatct cgacgtcgct cccgtcacct tactcatttg ctctattgtc
    38041 gcagcgttag cctcagtgct cggcgatctg accgagagta tgtttaagcg tgaagcagga
    38101 attaaggaca gcggtcattt aattccagga cacggtggta ttttagatcg tattgatagc
    38161 ctgacggctg cggtaccggt ctttgcttgc ttgttgttac tggtattcag gacgctttaa
    38221 cggaaggtaa tatgctgagt tttctctggg atttggcttc gttcatcgtt gcactgggtg
    38281 tacttatcac cgtgcatgaa tttggtcatt tctgggttgc ccggcgttgt ggtgttcgcg
    38341 ttgagcgttt ctcaataggg tttggtaagg cgctctggcg gcgaactgat aagctcggca
    38401 ccgaatatgt tatcgccctg atcccgttgg gcggttatgt caaaatgctg gatgagcgcg
    38461 cagaaccggt cgttccggaa ctccgccacc atgccttcaa taataaatct gtcggccaac
    38521 gagcggcgat tattgccgca ggtccggttg caaacttcat ttttgctatc tttgcctact
    38581 ggctggtttt tattattggt gtgcctggcg tacgtccggt ggttggcgaa atagcagcca
    38641 attcgatagc tgcggaagca caaattgcac caggtacgga actaaaagcc gtagatggta
    38701 tcgaaacgcc tgattgggat gccgtgcgtt tgcagttggt cgataaaatt ggcgatgaaa
    38761 gcaccaccat tacagtagcg ccatttggca gcgaccaacg gcgggatgta aagctcgatt
    38821 tacgtcactg ggcgtttgag cctgataaag aagatccggt atcttcgctg gggattcgtc
    38881 ctcgtgggcc gcaaattgaa cctgtactgg aaaatgtgca gccaaactcg gcggcaagca
    38941 aggcaggttt gcaagcaggc gacaggatcg ttaaagtcga tggtcagccc ttaacgcagt
    39001 gggtgacctt tgtgatgctt gtccgggata acccgggtaa atccttagcg ttagaaatcg
    39061 aaaggcaggg aagtcccttg tctttgacat taatcccgga gagtaaaccg ggtaatggta
    39121 aagcgattgg ttttgtcggt attgagccga aagtcattcc tttgccagat gagtataaag
    39181 ttgtacgcca gtatgggccg ttcaacgcca tcgtcgaagc cacggacaaa acgtggcagc
    39241 tgatgaagct gacggtcagt atgctgggaa aattgatcac cggtgatgtg aaactgaaca
    39301 acctcagtgg gccgatctct atcgccaagg gggctgggat gacagcggaa ctcggggtag
    39361 tttattacct gccgtttctt gcgcttatta gcgtgaactt agggataatt aacctgtttc
    39421 cgttgcccgt acttgacggg gggcatctgc tgttccttgc gatcgaaaag atcaagggcg
    39481 gaccggtatc cgagcgggtt caagactttt gttatcgcat tggctcgatt ctgctggtgc
    39541 tgttaatggg gcttgcactt ttcaatgatt tctctcggtt atgagagtta gttaggaaga
    39601 acgcataata acgatggcga tgaaaaagtt gctcatagcg tcgctgctgt ttagcagcgc
    39661 caccgtatac ggtgctgaag ggttcgtagt gaaagatatt catttcgaag gccttcagcg
    39721 tgtcgccgtt ggtgcggccc tcctcagtat gccggtgcgc acaggcgaca cggttaatga
    39781 tgaagatatc agtaatacca ttcgcgctct gtttgctacc ggcaactttg aggatgttcg
    39841 cgtccttcgt gatggtgata cccttctggt tcaggtaaaa gaacgtccga ccattgccag
    39901 cattactttc tccggtaaca aatcggtgaa agatgacatg ctgaagcaaa acctcgaggc
    39961 ttctggtgtg cgtgtgggcg aatccctcga tcgcaccacc attgccgata tcgagaaagg
    40021 tctggaagac ttctactaca gcgtcggtaa atatagcgcc agcgtaaaag ctgtcgtgac
    40081 cccgctgccg cgcaaccgtg ttgacctaaa actggtgttc caggaaggtg tgtcagctga
    40141 aatccagcaa attaacattg ttggtaacca tgctttcacc accgacgaac tgatctctca
    40201 tttccaactg cgtgacgaag tgccgtggtg gaacgtggta ggcgatcgta aataccagaa
    40261 acagaaactg gcgggcgacc ttgaaaccct gcgcagctac tatctggatc gcggttatgc
    40321 ccgtttcaac atcgactcta cccaggtcag tctgacgcca gataaaaaag gtatttacgt
    40381 cacggtgaac atcaccgaag gcgatcagta caagctttct ggcgttgaag tgagcggcaa
    40441 ccttgccggg cactccgctg aaattgagca gctgactaag atcgagccgg gtgagctgta
    40501 taacggcacc aaagtgacca agatggaaga tgacatcaaa aagcttctcg gtcgctatgg
    40561 ttatgcctat ccgcgcgtac agtcgatgcc tgaaattaac gatgccgaca aaaccgttaa
    40621 attacgcgtg aacgttgatg cgggtaaccg tttctacgtg cgtaagatcc gttttgaagg
    40681 taacgatacc tcgaaagatg ccgtcctgcg tcgcgaaatg cgtcagatgg aaggtgcatg
    40741 gctgggaagc gatctggtcg atcagggtaa ggagcgtctg aatcgtctgg gcttctttga
    40801 aactgtcgat accgataccc aacgtgttcc gggtagcccg gaccaggtag atgtcgtcta
    40861 caaggtaaaa gagcgcaaca ccggtagctt caactttggt attggttacg gtactgaaag
    40921 tggcgtgagc ttccaggctg gtgtgcagca ggataactgg ttaggtacag gttatgctgt
    40981 tggtatcaac gggaccaaaa acgattacca gacctatgct gaactgtcgg taaccaaccc
    41041 gtacttcact gtagatggtg taagcctcgg tggtcgtctc ttctataatg acttccaggc
    41101 agatgacgcc gacctgtccg actataccaa caagagttat ggtacagacg tgacgttggg
    41161 cttcccgatc aacgaatata actcgctgcg tgcaggtctg ggttatgtac ataactccct
    41221 gtccaacatg cagcctcagg ttgcgatgtg gcgttatctg tactctatgg gtgaacatcc
    41281 gagcacctct gatcaggata atagcttcaa aacggacgac ttcacgttca actatggttg
    41341 gacctataac aagcttgacc gtggttactt cccgacagat ggttcacgtg tcaacctgac
    41401 cggtaaagtg accattcctg gatcggataa cgaatactac aaagtgacgt tagacacggc
    41461 gacttatgtg ccgatcgatg acgatcacaa atgggttgtt ctggggcgta cccgctgggg
    41521 ttatggtgat ggtttaggcg gcaaagagat gccgttctac gagaacttct atgccggtgg
    41581 ttccagcacc gtgcgtggct tccagtccaa tacaattggt ccgaaagcag tttacttccc
    41641 gcatcaggcc agtaattatg atccggacta tgattacgaa tgtgcgactc aggacggcgc
    41701 gaaagacctg tgtaaatcgg atgatgctgt aggcggtaac gccatggcgg ttgccagcct
    41761 cgagttcatc accccgacgc cgtttattag cgataagtat gctaactcgg ttcgtacttc
    41821 cttcttctgg gatatgggta ccgtttggga tacaaactgg gattccagcc aatattctgg
    41881 atatccggac tatagtgatc caagcaatat ccgtatgtct gcgggtatcg cattacaatg
    41941 gatgtcccca ttggggccgt tggtgttctc ctacgcccag ccgttcaaaa agtacgatgg
    42001 agacaaggca gaacagttcc agtttaacat cggtaaaacc tggtaagtgt tctccacaaa
    42061 ggaatgtagt ggtagtgtag cgatgacttt aggcgatcaa tataagatcg ccgggccacg
    42121 caaagaactg caccctccgg tgcaaatggg atggtaagga gtttattgtg aaaaagtggt
    42181 tattagctgc aggtctcggt ttagcactgg caacttctgc tcaggcggct gacaaaattg
    42241 caatcgtcaa catgggcagc ctgttccagc aggtagcgca gaaaaccggt gtttctaaca
    42301 cgctggaaaa tgagttcaaa ggccgtgcca gcgaactgca gcgtatggaa accgatctgc
    42361 aggctaaaat gaaaaagctg cagtccatga aagcgggcag cgatcgcact aagctggaaa
    42421 aagacgtgat ggctcagcgc cagacttttg ctcagaaagc gcaggctttt gagcaggatc
    42481 gcgcacgtcg ttccaacgaa gaacgcggca aactggttac tcgtatccag actgctgtga
    42541 aatccgttgc caacagccag gatatcgatc tggttgttga tgcaaacgcc gttgcttaca
    42601 acagcagcga tgtaaaagac atcactgccg acgtactgaa acaggttaaa taagtaatgc
    42661 cttcaattcg actggctgat ttagcgcagc agttggatgc agaactacac ggtgatggcg
    42721 atatcgtcat caccggcgtt gcgtccatgc aatctgcaca aacaggtcac attacgttca
    42781 tggttaaccc aaaataccgt gagcatttag gcttgtgcca ggcgtccgcg gttgtcatga
    42841 cccaggacga tcttcctttc gcgaaaagtg ccgcgctggt agtgaagaat ccctacctga
    42901 cttacgcgcg catggcgcaa attttagata ccacgccgca gcccgcgcag aacattgcac
    42961 ccagtgcggt gatcgacgcg acggcgaagc tgggtaacaa cgtatcgatt ggcgctaacg
    43021 cggtgattga gtccggcgtt gaactgggcg ataacgtgat tatcggtgcc ggttgcttcg
    43081 taggtaaaaa cagcaaaatc ggtgcaggtt cgcgtctctg ggcgaacgta accatttacc
    43141 atgagatcca gatcggtcag aattgcctga tccagtccgg aacagtggta ggcgcagacg
    43201 gctttggtta tgccaacgat cgtggtaact gggtgaagat cccacagatt ggtcgcgtaa
    43261 ttattggcga tcgcgtggag atcggtgcct gcacaaccat cgatcgcggc gcgctggatg
    43321 acactattat tggcaatggc gtgatcattg ataaccagtg ccagattgca cataacgtcg
    43381 tgattggcga caatacggcg gttgccggtg gcgtcattat ggcgggcagc ctgaaaattg
    43441 gtcgttactg catgatcggc ggagccagcg taatcaacgg gcatatggaa atatgcgaca
    43501 aagtgacggt tacgggcatg ggtatggtga tgcgtcccat cactgaacca ggcgtctatt
    43561 cctcaggcat tccgctgcaa cccaacaaag tctggcgcaa aaccgctgca ctggtgatga
    43621 acattgatga catgagcaag cgtctgaaat cgcttgagcg caaggttaat caacaagact
    43681 aacgttccat cttttgttcg ccaaacttta cggcctgtct cattcttacg attgcggcag
    43741 gccgtgttat tattgtcgtt tcttatattt tgacaggaag agtatcttga ctactaacac
    43801 tcatactctg cagattgaag agattttaga acttctgccg caccgtttcc ctttcttact
    43861 ggtggatcgc gtgctggatt ttgaagaagg tcgttttctg cgcgcagtaa aaaatgtctc
    43921 tgtcaatgag ccattcttcc agggccattt ccctggaaaa ccgattttcc cgggtgtgct
    43981 gattctggaa gcaatggcac aggcaacagg tattctggcg tttaaaagcg taggaaaact
    44041 ggaaccgggt gagctgtact atttcgctgg tattgacgaa gcgcgcttca agcgcccggt
    44101 cgtgcctggc gatcaaatga tcatggaagt cactttcgaa aaaacgcgcc gcggcctgac
    44161 ccgttttaaa ggggttgctc tggtcgacgg taaagtagtt tgcgaagcaa cgatgatgtg
    44221 tgctcgtagc cgggaggcct gatacgtgat tgataaatcc gcctttgtgc atccaaccgc
    44281 cattgtggaa gagggcgcgt cgattggcgc gaacgcacac attggtcctt tttgtatcgt
    44341 tggaccccat gtcgaaattg gtgagggtac cgtactgaaa tctcacgttg tcgtgaatgg
    44401 ccatactaaa attggccgcg ataatgagat ttatcagttc gcctccatcg gtgaagttaa
    44461 ccaggatctg aaatatgctg gcgaaccgac ccgtgtggaa atcggcgatc gtaaccgcat
    44521 tcgcgaaagc gtcaccattc atcgtggcac agtccagggc ggtggattga cgaaggtggg
    44581 aagcgacaac ttactgatga tcaacgcgca cattgcgcac gattgtacgg taggtaaccg
    44641 ctgtattctc gccaacaacg caacgctggc gggtcacgta tcggtagacg acttcgcgat
    44701 catcggcggc atgaccgcag tccatcagtt ctgcatcatt ggtgcgcatg tgatggttgg
    44761 cggttgctcc ggtgtggcgc aggacgtccc tccttatgtc attgcgcagg gtaaccacgc
    44821 aacgccgttc ggtgtcaata tcgaagggct gaagcgccgc ggattcagcc gcgaggcgat
    44881 taccgctatc cgcaatgcgt ataagctgat ttatcgcagc ggtaaaacgc tcgatgaagt
    44941 gaaaccggaa attgctgaac tggcggaaac gtatccggaa gtgaaagctt ttaccgattt
    45001 ctttgcacgc tcaacgcgcg gtctgattcg ttaatgacta aacagcgtcc attaacgatt
    45061 gccctggtcg ccggagaaac ctccggcgat atcctggggg ccggtttaat ccgcgctctg
    45121 aaagaacgtg tgcccaacgc ccgctttgtt ggcgttgccg ggccacgaat gcaggctgaa
    45181 ggctgcgaag cctggtacga aatggaagaa ctggcggtga tgggcattgt tgaagtgctc
    45241 ggtcgtctgc gtcgcttact gcatattcgt gccgatctga caaagcgttt tggtgaacta
    45301 aagccagatg tttttgttgg cattgatgcg cctgacttca atattaccct cgaaggtaac
    45361 cttaaaaagc agggtatcaa aaccattcat tacgtcagtc catccgtctg ggcgtggcga
    45421 cagaaacgcg ttttcaaaat aggcagagcc accgatctgg tgctcgcatt tctgcctttc
    45481 gaaaaagcgt tttatgacaa atacaatgta ccgtgccgct ttatcggtca taccatggct
    45541 gatgccatgc cgttagatcc agataaaaat gccgcccgtg atgtgctggg gatccctcac
    45601 gatgcccact gcctggcgtt gctaccaggg agccgtggtg cagaagttga aatgcttagt
    45661 gccgatttcc tgaaaacggc ccagcttttg cgccagacat atccggatct cgaaatcgtg
    45721 gtgccactgg tgaatgccaa acgccgcgag cagtttgaac gcatcaaagc tgaagtcgcg
    45781 ccagaccttt cagttcattt gctggatggg atgggccgtg aggcgatggt cgccagcgat
    45841 gcggcgctac tggcgtcggg tacggcagcc ctggagtgta tgctggcgaa atgcccgatg
    45901 gtggtgggat atcgcatgaa gccttttacc ttctggttgg cgaagcggct ggtgaaaact
    45961 gattatgtct cgctgccaaa tctgctggcg ggtagagagt tagtcaaaga attattgcag
    46021 gaagagtgtg agccgcaaaa actggctgcg gcgctgttac cgctgttggc gaacgggaaa
    46081 accagccacg cgatgcacga taccttccgt gaactgcatc agcagatccg ctgcaatgcc
    46141 gatgagcagg cggcacaagc cgttctggag ttagcacaat gatcgaattt gtttatccgc
    46201 acacgcacct ggttgcgggt gtggatgaag tcggacgcgg gccgttagtt ggcgcggtcg
    46261 tcaccgctgc ggtgatcctc gacccggcgc gcccgattgc cgggctgaat gattccaaaa
    46321 agctgagcga aaaacgccgt ctggcgctct gtgaagagat caaagagaaa gcgttgagct
    46381 ggagtctggg ccgcgcggaa ccccacgaaa tcgacgagct gaacattctt catgcgacca
    46441 tgctggcgat gcagcgtgcc gtcgctgggc tgcatattgc gccggaatat gtgttgattg
    46501 atggtaaccg ctgcccgaaa ttaccgatgc ctgcgatggc tgtggtgaaa ggcgatagcc
    46561 gcgtaccgga aatcagtgcc gcgtctatcc tggcgaaagt gacgcgtgac gccgaaatgg
    46621 cggcgctgga tattgttttc ccgcaatatg gttttgccca acacaaaggg tatccaaccg
    46681 cttttcatct ggaaaaactg gctgaatatg gcgcgactga acatcatcgt cgcagctttg
    46741 ggcctgtcaa acgagcactg ggacttgcgt cctgattctt gtcgagatta agtaaaccgg
    46801 aatctgaaga tgtctgaacc acgtttcgta cacctgcggg tgcacagcga ctactcgatg
    46861 atcgatggcc tggccaaaac cgcaccgctg gtaaaaaagg cggcggcgtt gggtatgcca
    46921 gcactggcga tcaccgattt caccaacctt tgcggtctgg tgaagttcta cggagcggga
    46981 catggcgcag ggattaagcc tatcgtcggg gcagatttta acgtccagtg cgacctgctg
    47041 ggtgatgagt taacccacct gacggtactg gcggcgaaca ataccggcta tcagaatctg
    47101 acgttgctga tctcaaaagc gtatcagcgt gggtacggtg ccgctgggcc gatcatcgat
    47161 cgcgactggc ttatcgaatt aaatgaaggg ttgatccttc tttccggcgg gcgcatgggc
    47221 gacgtcggac gcagtctttt gcgtggtaac agcgcgctgg tagatgagtg tgtcgcgttt
    47281 tatgaagaac acttcccgga tcgctatttt ctcgagctga tccgcaccgg caggccggat
    47341 gaagaaagct atctgcacgc ggcggtggaa ctggcggaag cgcgcggttt gcccgtcgtg
    47401 gcgaccaacg acgtgcgctt tatcgacagc agcgactttg acgcacacga aatccgcgtc
    47461 gcgatccacg acggctttac cctcgacgat cctaaacgcc cgcgtaacta ttcgccgcag
    47521 caatatatgc gtagcgaaga ggagatgtgc gagctgtttg ccgacatccc agaagctctt
    47581 gccaacaccg ttgagatcgc caaacgctgt aacgtgactg tgcgtcttgg tgaatacttc
    47641 ctgccgcagt tcccgaccgg ggacatgagc accgaagatt atctggtcaa gcgtgcaaaa
    47701 gaaggcctgg aagagcgtct ggccttttta ttccctgatg aggaagaacg tcttaaacgc
    47761 cgcccggaat atgacgaacg tctggagact gaacttcagg ttatcaacca gatgggcttc
    47821 ccgggctact tcctcatcgt tatggaattt atccagtggt cgaaagataa cggcgtaccg
    47881 gtagggccag gccgtggctc cggtgcgggt tcactggtgg cctacgcgct gaaaatcacc
    47941 gacctcgatc cgctggaatt tgacctgctg ttcgaacgtt tccttaaccc ggaacgtgtc
    48001 tccatgcctg acttcgacgt tgacttctgt atggagaaac gcgaccaggt tatcgagcac
    48061 gtagcggaca tgtacggtcg tgatgcggta tcgcagatca tcaccttcgg tacaatggcg
    48121 gcgaaagcgg tgatccgcga cgtaggccgc gtgctggggc atccgtacgg ctttgtcgat
    48181 cgtatctcga aactgatccc gcccgatccg gggatgacgc tggcgaaagc gtttgaagcc
    48241 gagccgcagc tgccggaaat ctacgaagcg gatgaagaag ttaaggcgct gatcgacatg
    48301 gcgcgcaaac tggaaggggt cacccgtaac gccggtaagc acgccggtgg ggtggttatc
    48361 gcgccgacca aaattaccga ttttgcgccg ctttactgcg atgaagaggg caaacatccg
    48421 gtcacccagt ttgataaaag cgacgttgaa tacgccggac tggtgaagtt cgacttcctt
    48481 ggtttgcgta cgctcaccat catcaactgg gcgctggaga tgatcaacaa gcggcgggcg
    48541 aagaatggcg agccaccgct ggatatcgcc gcgatcccgc tggatgacaa gaaaagcttc
    48601 gacatgctgc aacgctcgga aaccacggcg gtattccagc ttgaatcgcg cggcatgaag
    48661 gacctgatca agcgtctgca acctgactgc ttcgaagata tgatcgcact ggtggcactg
    48721 ttccgccctg gtccgttgca gtcagggatg gtggataact ttattgaccg taaacatggt
    48781 cgcgaagaaa tctcctatcc agacgtacag tggcagcatg aaagcctgaa accggtactg
    48841 gagccaacct acggcattat cctgtatcag gaacaggtca tgcagattgc ccaggtgctt
    48901 tctggttata ccctcggtgg cgcggatatg ctgcgtcgag cgatgggtaa gaaaaagccg
    48961 gaagagatgg ctaagcagcg ttctgtattt gctgaaggtg cagaaaagaa cggaatcaac
    49021 gccgaactgg cgatgaaaat cttcgacctg gtggagaaat tcgcgggtta cggatttaac
    49081 aaatcgcact ctgcggccta tgctttggtg tcatatcaaa cgttatggct gaaagcgcac
    49141 tatccggcgg agtttatggc ggcggtaatg accgccgata tggacaacac cgagaaggtg
    49201 gtgggcctgg tggatgagtg ctggcggatg gggctgaaaa tcctgccacc agatataaac
    49261 tccggtcttt accatttcca cgtcaacgac gacggcgaaa tcgtgtatgg tattggcgcg
    49321 atcaaagggg tcggtgaagg tccgattgag gccatcatcg aagcccgtaa taaaggcggc
    49381 tacttccgcg aactgtttga tctctgcgcc cgaaccgaca ccaaaaagtt aaaccggcgg
    49441 gtgctggaaa aactgatcat gtccggggcg tttgaccgtc ttggaccaca ccgcgcggca
    49501 ctgatgaact cgctgggcga tgcgttaaaa gcggcagatc aacacgcgaa agcggaagct
    49561 atcggtcagg ccgatatgtt cggcgtgctg gccgaagagc cggaacaaat tgaacaatcc
    49621 tacgccagct gccaaccgtg gccggagcag gtggtattag atggggaacg tgaaacgtta
    49681 ggcctgtacc tgaccggaca ccctatcaac cagtatttaa aagagattga gcgttatgtc
    49741 ggaggcgtaa ggctgaaaga catgcacccg acagaacgtg gtaaagtcat cacggctgcg
    49801 gggctcgttg ttgccgcgcg ggttatggtc accaagcgcg gcaatcgtat cggtatctgc
    49861 acgctggatg accgttccgg gcggctggaa gtgatgttgt ttactgacgc cctggataaa
    49921 taccagcaat tactggaaaa agaccgcata cttatcgtca gcggacaggt cagctttgat
    49981 gacttcagcg gtgggcttaa aatgaccgct cgcgaagtga tggatattga cgaagcccgg
    50041 gaaaaatatg ctcgcgggct tgctatctcg ctgacggaca ggcaaattga tgaccagctt
    50101 ttaaaccgac tccgtcagtc tctggaaccc caccgctctg ggacaattcc agtacatctc
    50161 tactatcaga gggcggatgc acgcgcgcgg ttgcgttttg gcgcgacgtg gcgtgtctct
    50221 ccgagcgatc gtttattaaa cgatctccgt ggcctcattg gttcggagca ggtggaactg
    50281 gagtttgact aatacaggaa tactatgagt ctgaatttcc ttgattttga acagccgatt
    50341 gcagagctgg aagcgaaaat cgattctctg actgcggtta gccgtcagga tgagaaactg
    50401 gatattaaca tcgatgaaga agtgcatcgt ctgcgtgaaa aaagcgtaga actgacacgt
    50461 aaaatcttcg ccgatctcgg tgcatggcag attgcgcaac tggcacgcca tccacagcgt
    50521 ccttataccc tggattacgt tcgcctggca tttgatgaat ttgacgaact ggctggcgac
    50581 cgcgcgtatg cagacgataa agctatcgtc ggtggtatcg cccgtcttga cggtcgtccg
    50641 gtgatgatca ttggtcatca aaaaggtcgt gaaaccaaag aaaaaattcg ccgtaacttt
    50701 ggtatgccag cgccagaagg ttaccgcaaa gcactgcgtc tgatgcaaat ggctgaacgc
    50761 tttaagatgc ctatcatcac ctttatcgac accccggggg cttatcctgg cgtgggcgca
    50821 gaagagcgtg gtcagtctga agccattgca cgcaacctgc gtgaaatgtc tcgcctcggc
    50881 gtaccggtag tttgtacggt tatcggtgaa ggtggttctg gcggtgcgct ggcgattggc
    50941 gtgggcgata aagtgaatat gctgcaatac agcacctatt ccgttatctc gccggaaggt
    51001 tgtgcgtcca ttctgtggaa gagcgccgac aaagcgccgc tggcggctga agcgatgggt
    51061 atcatcgctc cgcgtctgaa agagctgaaa ctgatcgact ccatcatccc ggaaccgctg
    51121 ggcggcgctc accgtaaccc ggaagcgatg gcggcatcgt tgaaagcgca actgctggcg
    51181 gatctggccg atctcgatgt gttaagcact gaagatttaa aaaatcgtcg ttatcagcgc
    51241 ctgatgagct acggttacgc gtaattcgca aaagttctga aaaagggtca cttcggtggc
    51301 ccttttttat cgccacggtt tgagcaggct atgattaagg aaggattttc caggaggaac
    51361 acatgaacat cattgccatt atgggaccgc atggcgtctt ttataaagat gagcccatca
    51421 aagaactgga gtcggcgctg gtggcgcaag gctttcagat tatctggcca caaaacagcg
    51481 ttgatttgct gaaatttatc gaacataacc ctcgaatttg cggcgtgatt tttgactggg
    51541 atgagtacag tctcgattta tgcagcgata tcaatcaact taatgaatat ctcccgcttt
    51601 atgccttcat caacacccac tcgacgatgg atgtcagcgt gcaggatatg cggatggcgc
    51661 tctggttttt tgaatatgcg ctggggcagg cggaagatat cgccattcgt atgcgtcagt
    51721 acaccgacga atatcttgat aacattacac cgccgttcac gaaagccttg tttacctacg
    51781 ttaaagagcg gaagtacacc ttttgtacgc cggggcatat gggcggcacc gcatatcaaa
    51841 aaagcccggt tggctgcctg ttttatgatt ttttcggcgg gaataccctc aaggctgatg
    51901 tctctatttc ggtcaccgag cttggttcgt tgctcgacca caccgggcca cacctggaag
    51961 cggaagagta catcgcgcgg acttttggcg cggaacagag ttatatcgtt accaacggaa
    52021 catcgacgtc gaacaaaatt gtgggtatgt acgccgcgcc atccggcagt acgctgttga
    52081 tcgaccgcaa ttgtcataaa tcgctggcgc atctgttgat gatgaacgat gtagtgccag
    52141 tctggctgaa accgacgcgt aatgcgttgg ggattcttgg tgggatcccg cgccgtgaat
    52201 ttactcgcga cagcatcgaa gagaaagtcg ctgccaccac gcaggcacaa tggccggttc
    52261 atgcggtgat taccaactcc acctatgatg gcttgctcta caacaccgac tggatcaaac
    52321 agacgctgga tgtcccgtcg atccacttcg attccgcctg ggtgccgtac acccattttc
    52381 atccgatcta ccaggggaaa agtggtatga gcggcgagcg tgttgcggga aaagtgatct
    52441 tcgaaacgca atcgacccac aaaatgctgg cggcgttatc gcaggcttcg ctgatccaca
    52501 ttaaaggcga gtatgacgaa gaggcgttta acgaagcctt tatgatgcat accaccacct
    52561 cgcccagtta tcccattgtt gcttcggttg agacggcggc ggcgatgctg cgtggtaatc
    52621 cgggcaaacg gctgattaac cgttcggtgg agcgggcgct gcattttcgc aaagaggtcc
    52681 agcggttgcg ggaagagtct gacggttggt ttttcgatat ctggcaaccg ccgcaggtgg
    52741 atgaagccga atgctggccc gttgcgccag gcgaacagtg gcacggcttt aacgatgcgg
    52801 atgccgatca tatgtttctc gatccggtta aagtcactat tttgacaccg gggatggacg
    52861 aacagggcaa tatgagcgag gaggggatcc cggcagcgct ggtggcaaaa ttcctcgacg
    52921 aacgtgggat cgtagtagag aaaaccggcc cttataacct gctgtttctc tttagtattg
    52981 gtatcgataa aaccaaagca atgggattat tgcgtgggtt gacggaattt aaacgctctt
    53041 acgatctcaa cctgcggatc aaaaatatgc tacccgatct ctatgctgaa gatcccgatt
    53101 tctaccgcaa tatgcgtatt caggatctgg cgcaagggat ccataagctg attcgtaaac
    53161 acgatcttcc cggtttgatg ttgcgggcat tcgatacttt gccggaaatg atcatgacgc
    53221 cacatcaggc atggcagaga cagattaaag gtgaagtaga aaccattgcg ctggaacaac
    53281 tggtcggtag agtatcggca aatatgatcc tgccttatcc accgggcgta ccgctgttga
    53341 tgccaggaga aatgctgacc aaagagagcc gcacggtact cgattttcta ctgatgcttt
    53401 gttccgtagg gcaacattac cccggttttg aaacggatat tcacggcgcg aaacaggacg
    53461 aagacggcgt ttaccgcgta cgagtcctaa aaatggcggg ataacttgcc agagcggctt
    53521 ccgggcgagt aacgtgctgt taacaaataa aggagacgtt atgctgggtt taaaacaggt
    53581 tcaccatatt gcgattattg cgacggatta tgcggtgagc aaagctttct actgcgatat
    53641 tcttggtttc acgctgcaaa gcgaagtcta tcgcgaagcg cgcgactcgt ggaaagggga
    53701 tttggcgctt aatgggcaat atgtgattga gcttttctct tttccgttcc cgccggagcg
    53761 ccccagccga ccggaagctt gcggcctgcg tcatctggct tttagcgttg atgatatcga
    53821 tgcggcagtg gcgcaccttg aaagccataa cgtgaagtgt gaagccatcc gtgtcgatcc
    53881 atacacgcaa aaacgcttca cgttctttaa cgatccggac gggctgccgt tggaactgta
    53941 tgagcagtaa ggcttgtcat cgccgcattt gcccggtaac gtgccgggca ttgctactgt
    54001 aaaatcgcac catcatgaca ctcacgctca atagacaact tctcacctta cgccagattc
    54061 tggtggcctt tagcggcggg cttgactcca ccgttctgct gcatcagttg gtgcagtggc
    54121 ggacggaaaa tccgggcgtc actctacgcg ctatccatgt gcatcacggt ttaagtgcca
    54181 atgccgatgc ctgggttagg cattgcgaga acatctgcca acagtggcag gtgccgctgg
    54241 tggtcgagcg cgtgcaactt gcgcaagaag gactgggcat tgaggcccag gcgcggcagg
    54301 cgcgttatca ggcatttgcc cgcaccttgt tgcccggtga agtgctggtc accgcgcaac
    54361 atctcgacga tcaatgtgaa acttttctgc tggcgctaaa acgcggtagc ggccctgccg
    54421 ggctttcggc tatggcggaa gtttcggagt ttgccggaac acggcttatc cgcccgttgc
    54481 ttgcccgcac gcggggggaa ctggcgcagt gggcattggc gcatggttta cgctggattg
    54541 aagacgaaag taatcaggac gactcatacg atcgtaactt tctgcgcctg cgcgtagtgc
    54601 cgttattgca gcagcgttgg ccgcattttg ccgaagcaac ggcccgcagc gctgcgcttt
    54661 gcgctgaaca agagagcctg ttagatgaac tgctggcgga tgatatagcg cactgtcaaa
    54721 agccgcaggg gacgctgcag attgcgccaa tgctggcgat gagtgatgcc cgtcgcgcgg
    54781 cgattatccg ccgctggctg gcagggaaga atgcaccgat gccttcccgc gacgcgttgg
    54841 tgcggatctg gcaggaagtg gcgctggcgc gggaagatgc ctccccctgt ttacgcttgg
    54901 gcgcgtttga aatccgccgc tatcagtcgc aactgtggtg gattaaatcc gtcaccgggc
    54961 aaagtgaaac cattgtgccg tggcagacgt ggctacaacc gctggaatta ccggcggggc
    55021 tgggaagcgt acagcttact gcgggaggcg atattcgccc tccgcgtgca gacgaagcgg
    55081 tcagcgtgcg tttcaaagcg ccaggattgc tgcatattgt cggtcgcaac ggcggacgta
    55141 agctaaagaa aatctggcaa gagctgggcg tgccgccgtg gctacgtgac accacgccac
    55201 tgctgtttta tggcgaaacg ctgattgcgg cggcaggggt atttgtgacg caagaaggtg
    55261 tggctgaagg tgagaagggc gtaagttttg tctggcagaa aacgcttagt taagtgaaag
    55321 ccggataaga cgcatcaaac gtcgcatccg gcgaaagtca atcaggattc gcttaccacc
    55381 accgtaccga tttccgggtg gctaaagctg gtaattttat ccagacgcag ctcgcgggtt
    55441 tcgccagcgg cctcgacgac tagatactcc acatttttgc gggagactaa atcactggct
    55501 tttgcctgca atttttcgcc atctttcagc tcaagtgtca gcattaaatg atgctggcag
    55561 gcgagctcaa gattatcgta atcatcacaa ttgattggtt gatacgtatc attcattgac
    55621 ataatcgctc accagtaagt ttgccgcagc gtatgctgct ttttccctga cagcctcaga
    55681 aagggcgtcg tcggcagcca tttcattcag cactttcaaa acgcagccca gcgcgtccgg
    55741 aacgtatcct aagtctccgc tggcgatttc cgcgtaccgc ttgcgtatta actcacaata
    55801 tttttccaca tgccctcctg tcagcactct gacttaaccg tggatgcaag tctaagccta
    55861 cgaagataaa ctctgtttcg caaggtgact ataccacact catttctgca atatcagcgc
    55921 cgcaactgca cgtattccgt tacaatggcc tcctgattcg aaaggagttt tcttatggcg
    55981 cttaaagcga caatttataa agcgacggtt aatgtggccg atctcgaccg taaccagttt
    56041 ctcgatgcct ctctgacgct ggcgcgccac ccttcagaaa cccaggagcg tatgatgctg
    56101 cgcttgctgg cgtggttgaa atatgctgat gaacgtctgc aatttacccg tggtttgtgt
    56161 gccgatgatg agccggaagc gtggctgcgt aacgatcacc tgggcattga tttgtggatt
    56221 gagctggggc tgccggatga gcggcggatt aagaaagcct gcacccaggc cgcaaaagtg
    56281 gcgctgttta cctataatag ccgggcggcg caaatctggt ggcagcaaaa tcagagcaaa
    56341 tttgtgcagt ttgccaatct ttccgtctgg tatctggacg atgaacaact ggcgaaagta
    56401 agcgcctttg ccgatcgtac catgacgctg caggcaacga ttcaggatgg cgtgatctgg
    56461 ttatcggatg ataagaataa tctggaagtg aacttaaccg cctggcaaca accttcatga
    56521 ttgtgatttc cctacatgtt gctattcccg atggtgagct tgagatcacc gccattcgtg
    56581 cgcagggcgc gggcgggcag catgttaata agacctcaac ggctattcat ctgcgttttg
    56641 acattcgggc gtccagcctg ccagagtatt acaaagagcg tctgctcgcc gccagccatc
    56701 atttgatcag cagtgatggc gtgattgtca ttaaggcaca ggaataccgc agtcaggaac
    56761 tgaaccgcga agcggcgctg gcccggctgg tggcagtgat taaagattta acaacagaac
    56821 aaaaagcccg acgacccacg cggcccaccc gtgcatcgaa agagcgcagg ctggcatcga
    56881 aagcacaaaa atcaagcgtg aaggcgatgc gcggcaaagt acgcagcggt cgggaataaa
    56941 aagaaggaat ggatggtgaa aaaagcgata gtgacagcga tggctgtaat cagcctcttt
    57001 actctgatgg gatgtaataa tcgggccgaa gtcgatacgc tttctccggc gcaggctgcc
    57061 gaactaaaac cgatgccgca aagttggcgc ggcgtgctgc cgtgtgccga ttgcgaaggg
    57121 atcgaaacct ctctgttcct cgaaaaagac ggaacatggg tgatgaatga gcgttatctc
    57181 ggtgctcgtg aagaaccttc ctccttcgct tcctacggta catgggcgcg aaccgctgac
    57241 aagctggtat taaccgacag caaaggtgaa aagtcatatt atcgggcgaa aggcgatgcg
    57301 ctggagatgc tcgatcgtga aggcaatcca attgaatcgc agttcaacta tacgctggaa
    57361 ccggcacaat ccagtttacc tatgacgccg atgaccctgc gaggcatgta tttttatatg
    57421 gctgatgcgg cgaccttcac tgattgcgcg accggaaaac gtttcatggt agcgaataac
    57481 gcagagctgg agcgtagcta cctggctgcg cgcggtcaca gtgaaaaacc ggtgttactg
    57541 tcagtagaag gtcactttac gcttgaggct aatccggata ctggtgcgcc aaccaaagta
    57601 ttagcgcccg atacggcggg taaattttac ccgaataagg attgcagtag tttggggcag
    57661 taacccgtct tgagacagaa acaaacgcaa aattgcctga tgcgctgcgc ttatcaggcc
    57721 tacagcatat aatgcaacat tttgaattag cacgattttg taggccggat aaggcgttta
    57781 cgccgcatcc ggcattttgt gcacgaggct ggctttatca ctgagtcagc ccaacaaagc
    57841 gacccgcctt gatataaatc ccaggcttca ggtgcccaac atactgtaac tgcgtttctg
    57901 gcttaaatgc agagacatcg ccagtacgca tatgcatcag atcggcaggg ctaatccagc
    57961 ccgattgcgc cagcgtaagc ggatgcccgg ctttggcaaa tgcatcagtg acaaactccg
    58021 aacaaaacca cgactttttg tctccttcgc ccacactgct taactgcgct ttcgccaggc
    58081 cgctgacgca ctgttggcga aaatctgcgg aaaaaggatt cagtgagcac atctggcggg
    58141 tcaccataaa tggaatgaac tcgacaatgc cgcgatagtt atacccgcta tctttgattt
    58201 tgttggcgaa cgcggtgatt tctgtggctt gttgcggggt aagatccggg actcgtaaga
    58261 cgaaaagctt atcgctatgc ttcatcgctt ttttaaggga aacaatctgg acgccagcgc
    58321 ctgtcgcttc tgcaacgtta ttttcaccca gatagattgc aacgtgactc acagaggaag
    58381 tgctgaagac gcggattcca aatgaggtta ccccaaggct tgaggagaac agcaaatcgc
    58441 cgggtttgag atcaggtgcc gttatttctt ttattgattg ttcggtgaaa gagctttgat
    58501 gctggaattt gacagcccat gtttttgcct cggcatctac tgctgtggct gatgggtctg
    58561 gctggctgat atcaacggta caagccgaaa gtaaaagaaa gctggggaga agcaggcggc
    58621 agtacgcctt tggtttatcc attttataca atccatgtaa aaaagggccc tgaaattcag
    58681 gaccctttct ggcatcagcc tttaatctgt ttcaccagat attcgacgat gtcaccagtc
    58741 ttaattaact gtttctcgcc gttgcgacga tatttatatt cgatatcgtc gttgtcgagg
    58801 ttacggtcgc ccagcacaat agtgtgcgga ataccgatca gttccatatc agcaaacatc
    58861 acgcccggac gctctttgcg gtcatccagc agcacttcga taccttgtgc acgcagttcg
    58921 ctgtacagtt tctcagcaag ctcttgtacg cggaaggatt tgtgcatgtt catcggcaga
    58981 atcgccacct ggaacggcgc gatcgcgtca ggccatacga tgccgcgttc gtcgtagttc
    59041 tgctcgatcg ccgcagctac tacacgcgtt accccgatac cgtagcaacc catcgtcagg
    59101 atttgattac ggccatcttc accctgtacg gaggctttca gtgcttcgga gtacttggta
    59161 cccagctgga agatgtgacc aacttcgata ccacgtttga tcagcagcgt accctgacca
    59221 tccgggcttg gatcgcccgc caccacgttg cggatatcag caatttccgg ggtagcgaca
    59281 tcgcgatccc agttgatacc gaagtagtgt ttaccatcga tgttagcacc agcagcgaaa
    59341 tcactcatcg ccgcaacggt acggtcaatc accaccggaa tcggcatgtt taccggaccc
    59401 agtgaacccg gaccggcttt aaccacggca cgaatttctt cttcagtcgc aaaagtcagc
    59461 gggctggcaa cctgcggcag tttttctgct ttaacttcgt tcagctcgtg gtcaccgcgc
    59521 accagcagcg caaccaacgg gaagctactg ccttcaaccg ctttaaccag cagagtctta
    59581 accgttttct caatcggcag attgaactgt tcaaccagtt ccgcgatggt tttcgcgttc
    59641 ggcgtatcaa ccagcgtcat ttcctgggta gccgcagcgc gcggttcttt cggcgcgata
    59701 gcttctgcca gttcaatgtt cgctgcatag tcagaggtgt cggagaagac cacatcgtct
    59761 tcaccgctct gcgccagcac ctggaattcg tgagaggcgc tgccgccgat agaaccggtg
    59821 tcggcctgta cggcgcggaa atccagcccc atgcggctga agattttgct gtaggccgca
    59881 tacattgcat cgtaggtttc ctgcagggat tcctgagaag tatggaaaga gtaagcatct
    59941 ttcatcagga attcgcggga acgcatgacg ccgaaacgcg gacgcacttc gtcgcggaac
    60001 ttggtctgga tctgatagaa gttcagcggc agctgtttgt aagagctaag ctcgttacga
    60061 atcaggtcag tgataacttc ttcatgagtt gggccgagta cgaacggacg ctcgccacgg
    60121 tcaacaaaac gcagcagttc cggaccgtac tgttcccaac gaccactctc ttgccacaaa
    60181 tcggctggct gaaccaccgg catcgacacc tcgattgcac cggcgttgtt catctcttca
    60241 cgcacgatgt tttcgacttt tttcagaacg cgcacgccgg tcggcagcca ggtatataac
    60301 ccggaggcca gcttgcggat catcccggcg cgcagcatca gctgatggct gatcacctcg
    60361 gcgtcggcag gtgtctcctt gagagtggag agcaggtatt ggctagtacg catgttgtta
    60421 cggttccagt tggaaggtag aacaggctca aggcgagcct gggacaaaaa aagtgattta
    60481 gtttaccagt gcaaaagaaa tgtcaaaaga gaagggcgtg aatttaacgc ggttccagcg
    60541 caaagacttc aaaacctgcg tcggtgacgc gccagcgaac gttaaaatca tgtagccaga
    60601 cggcataggt tttgcccgtt tcctcacctt tacgataggc cgggcgcggg tcctgcgcca
    60661 gtacttcgcg gataaacagc gttaactgcg gataacgctt ctccagcgtc aaaagctgct
    60721 tttcgacctc tgcggtaaaa ctcaccgcca tttctgccgc tggcgcactt tgcgcatagc
    60781 tggcactggc atcgggaagc gattcggcaa aggggagata cggtttgata tccactaccg
    60841 gcgtaccatc gaccagatcc agactgccga gcttcagaat cacgctgtct ttatggcaaa
    60901 caacctcttt cagctctacc agcgacatac caattgggtt agggcggaaa gtagagcgcg
    60961 tagcgaaaac acccattctg gcattaccgc caagacgagg cggacgcaca gtcggacgcc
    61021 agccgccttc catcgtttga tgaaagacga aaagtatcca taaatggctg aacgcttcca
    61081 ggccgcgaac ggcgtcggcc tggttgtagg gagccatgag atgcagttcg ccgttggcgc
    61141 tttttaccag acctggctgg cgcggaacgg cgaatttttc tttatagggc gagcgaataa
    61201 cgcctatttg ctcgaactgg aaactgctca tttcgccgta atgttaagcg cagagccgat
    61261 acatacagcc tgacgatagc agcctggcgt accgctggta acttcgcagc tatgcagtaa
    61321 taccgcattg gctttcattt tcgaggcgtt gatttgcatc cgcttacgtg cggttggaat
    61381 gctcggcgga gagtcctgat tagaggcctg gcaagagtcg ccactgactt caccgagatc
    61441 gcggaacggt ttgccgacta attcttctgc attggtataa attcggaccg gcgtggcgcg
    61501 cggcgctttc ggttttgcag gctccgcttt cggctggggt gcagtgcttt gaacgggttc
    61561 gacaggggat ctgcttaaca tggaacagcc gcttagcatg agtgctacta aacagatcgg
    61621 taaagcacgc atagtatttc ctcaatgtat gatcaaaacg tcaatattga atcaggagct
    61681 tgtaaaaatg acaagacggg caagcgcccg tcctgaatga tattacaaat tgtggaaaca
    61741 gcctaaaaat taccagcctt taacagcgcc gccgttaaac actttgtttg ctgcttcgta
    61801 aacttcatca gactgataag cctggacgaa tttcttcacg ttctcggcgt ctttgttatc
    61861 ttcacgcgtt acgatcaggt ttacgtacgg ggagtcttta tcttcgacaa agataccgtc
    61921 tttcgctgga gtcaggccaa tctggctggc ataggtggta ttgataactg ccagagcgat
    61981 ttgcgcgtcg tccagagatc gaggcagctg cggtgcttcc agctcaacaa ttttcagatt
    62041 ttttgggttc tcaacaacat caagaacggt cggcagcagg ccaacgccat ctttcagttt
    62101 gatcaagccc actttttgca gcagcagcag tgaacgacca agattagttg gatcgtttgg
    62161 cacggcaacc tgcgaaccat cctgcagttc atccagtgat ttgattttct tggagtaacc
    62221 agcaatcgga taaacaaaag tgttgcctac tgcgaccagt ttgtagccac gatctttcag
    62281 ttgctgatca aggtacggtt tatgctggaa ggcgttggcg tcgatatcgc ctttgctcaa
    62341 tgcttcgttt ggcagaacat agtcgttgaa ggttaccagc tcaacgtcca ggccatattt
    62401 gtctttcgca actttctgcg cgacttctgc tacctgctgt tcggcaccaa caatcacgcc
    62461 gactttaatg tggtttggat ctttttcatc ctgaccgcag cctaccagtg ccagtgatcc
    62521 aatcagggct cccactgccg caaaggtttt gaatttgaac gccatacctt attccttctt
    62581 caattattta tgttgtgttg aacgttactt gcgagtgaca gcccggacga tgcggtcgcc
    62641 tgcgaactga attaaataaa ccagaatgac cagcaatacc agtaccgtat tcatcaccgt
    62701 cgcgttatag ccgatgtagc catactgata gccaatctga cctaaaccac cggcaccgac
    62761 tgcaccaccc atcgcggaat aaccgaccag ggtaatcagg gtgatagttg ccgcattcac
    62821 cagacccggc agcgcttccg gtaacagcac cttacggacg atctgcatcg gcgtggcacc
    62881 cattgcgcgg gaagcttcaa ttaacccggt tgggatctct agcagagcgt tctcgaccat
    62941 acgggcaata aacggcgctg cgccaacggt taacggaaca atcgccgcct gtaaaccaat
    63001 cgatgtaccg acaataactc gggtaaacgg aatcatccat accagcaaga taatgaacgg
    63061 gatggaacgg aaaatgttca caattgcaga aacggtacga tacagcttcg cgttagcaat
    63121 aatttgcccc ggacgcgtga cataaagcag aacgccaacc ggcagaccaa tcacaaagcc
    63181 aaaaaaaccg gatacgaagg tcattgccag cgtttcccat acgccacgaa ccagcagcca
    63241 catcatcggc tcagacataa cccagtacct ctacttttac atggtgttcc tgcagccagg
    63301 caatggcggc ttgcgtatct tgttgtgtgc cgtgcatttc ggtcagcatg atgccgaact
    63361 taacgccacc ggcgtaatcc atctgcgcgc taataatgtt gttgttgacg ttgaaacgac
    63421 gcgcggtttc agaaagcagt ggggcatcga ccgattgacc ggtaaactcc agacgcagca
    63481 tcggcacgca gtcagtaaat ggctccgctt gcagacgttc ctggtaatct tccgggatat
    63541 ccagatgcag ggtcgactga ataaacttct gcgccagcgg cgttttcgga tgcgagaaca
    63601 cttcacttac cgtgtcctgc tcgatcagtt cgccattgct gatgaccgcc acgcaatcac
    63661 aaatgcgctt cacaacgtcc atttcgtgag tgatcaacag aatcgtcaaa cccagacggc
    63721 ggttgatgtc tttcagcagt tcgagaatag aacgtgtcgt tgccgggtcc agcgcgctgg
    63781 tggcttcatc acacagcaat actttgggat tgctggctaa tgcacgggca atcgccacac
    63841 gttgtttctg cccaccggaa agattcgacg ggtagctatc atgcttatcg ccaagaccaa
    63901 ccaatgacag caattccgtc acgcgacgtt tgatctcgtc tttcggtgtg ttgtccagct
    63961 ccagcggcag agccacgttg ccaaaaacag tacgcgaaga gagcaggtta aaatgctgga
    64021 aaatcatacc aatctggcgg cgagctttgg tcaactcgga ttctgacagc gtggtcagtt
    64081 cctggccatc gaccagcacg ctaccctcgg ttgggcgctc cagcaggttt acacaacgta
    64141 taagcgtact cttacccgcg cctgaggcac cgataacgcc ataaatttgt ccagctggca
    64201 catgcaggct gacgttgttc aacgcctgga tggtgcgggt gccctggtgg aacactttgg
    64261 tgatattcga aagttttatc attgattatt tattatcgtc attaagttag tcgtggcatc
    64321 tcgaatgcct gaaacgggca acgccgtcaa tgaaatggat gttaaggcat ccagacgtct
    64381 aaatcaatca ggtttatgcg aagagcactt tcttgcaggt cgaaacatgc gatactagcg
    64441 tcacatgcct tattaaggag ctataaaagg tggcgaagag cgtacccgca atttttcttg
    64501 accgtgatgg caccattaat gtcgatcacg gctatgtcca tgagatcgac aactttgaat
    64561 ttatcgacgg tgttattgac gccatgcgcg agctaaaaaa aatgggcttt gcgctggtgg
    64621 tagtaaccaa ccagtctggc attgctcgcg gtaaatttac cgaagcacag tttgaaacgc
    64681 tgaccgagtg gatggactgg tcgctggcgg accgagatgt cgatctggat ggtatctatt
    64741 attgcccgca tcatccgcag ggtagtgttg aagagtttcg ccaggtctgc gattgccgca
    64801 aaccacatcc ggggatgctt ttgtcagcac gcgattattt gcatattgat atggccgctt
    64861 cttatatggt gggcgataaa ttagaagata tgcaggcagc ggttgcggcg aacgtgggaa
    64921 caaaagtgct ggtgcgtacg ggtaaaccta ttacacctga agcagaaaac gcggcagatt
    64981 gggtgttaaa tagcctggca gacctgccgc aagcgataaa aaagcagcaa aaaccggcat
    65041 aatgattaaa agatgagcga ttgaaataaa aatgcatttt tccacttgtc ttcctgagac
    65101 gactccctat aatgcgcctc catcgacacg gcggatgtga atcacttcac acaaacagcc
    65161 ggttcggttg aagagaaaaa tcctgaaatt ca
//
LOCUS       DBANUU010000006        63493 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000006 DBANUU010000000
VERSION     DBANUU010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.006"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            141..476
                     /gene="eutS"
                     /locus_tag="ACIE68_000352"
     CDS             141..476
                     /gene="eutS"
                     /locus_tag="ACIE68_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFX5906728.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            489..968
                     /gene="eutP"
                     /locus_tag="ACIE68_000353"
     CDS             489..968
                     /gene="eutP"
                     /locus_tag="ACIE68_000353"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFX5906729.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            943..1644
                     /gene="eutQ"
                     /locus_tag="ACIE68_000354"
     CDS             943..1644
                     /gene="eutQ"
                     /locus_tag="ACIE68_000354"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFX5906730.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1641..2444
                     /gene="eutT"
                     /locus_tag="ACIE68_000355"
     CDS             1641..2444
                     /gene="eutT"
                     /locus_tag="ACIE68_000355"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFX5906731.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            2441..3457
                     /gene="pta"
                     /locus_tag="ACIE68_000356"
     CDS             2441..3457
                     /gene="pta"
                     /locus_tag="ACIE68_000356"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFX5906732.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTKVNRESSLQ
                     TLVE"
     gene            3496..3789
                     /gene="eutM"
                     /locus_tag="ACIE68_000357"
     CDS             3496..3789
                     /gene="eutM"
                     /locus_tag="ACIE68_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFX5906733.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3896..4183
                     /gene="eutN"
                     /locus_tag="ACIE68_000358"
     CDS             3896..4183
                     /gene="eutN"
                     /locus_tag="ACIE68_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFX5906734.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4195..5598
                     /locus_tag="ACIE68_000359"
     CDS             4195..5598
                     /locus_tag="ACIE68_000359"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFX5906735.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            5609..6445
                     /gene="eutJ"
                     /locus_tag="ACIE68_000360"
     CDS             5609..6445
                     /gene="eutJ"
                     /locus_tag="ACIE68_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFX5906736.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            6435..7622
                     /gene="eutG"
                     /locus_tag="ACIE68_000361"
     CDS             6435..7622
                     /gene="eutG"
                     /locus_tag="ACIE68_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFX5906737.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGLGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWLCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            7739..8965
                     /gene="eutH"
                     /locus_tag="ACIE68_000362"
     CDS             7739..8965
                     /gene="eutH"
                     /locus_tag="ACIE68_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFX5906738.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            8962..10365
                     /gene="eutA"
                     /locus_tag="ACIE68_000363"
     CDS             8962..10365
                     /gene="eutA"
                     /locus_tag="ACIE68_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFX5906739.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPINETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            10377..11738
                     /gene="eutB"
                     /locus_tag="ACIE68_000364"
     CDS             10377..11738
                     /gene="eutB"
                     /locus_tag="ACIE68_000364"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFX5906740.1"
                     /translation="MKLKTTLFSNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            11759..12646
                     /gene="eutC"
                     /locus_tag="ACIE68_000365"
     CDS             11759..12646
                     /gene="eutC"
                     /locus_tag="ACIE68_000365"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFX5906741.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            12656..13315
                     /gene="eutL"
                     /locus_tag="ACIE68_000366"
     CDS             12656..13315
                     /gene="eutL"
                     /locus_tag="ACIE68_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFX5906742.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKYADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            13328..13828
                     /gene="eutK"
                     /locus_tag="ACIE68_000367"
     CDS             13328..13828
                     /gene="eutK"
                     /locus_tag="ACIE68_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFX5906743.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            13874..14926
                     /gene="eutR"
                     /locus_tag="ACIE68_000368"
     CDS             13874..14926
                     /gene="eutR"
                     /locus_tag="ACIE68_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFX5906744.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(14932..15831)
                     /gene="hemF"
                     /locus_tag="ACIE68_000369"
     CDS             complement(14932..15831)
                     /gene="hemF"
                     /locus_tag="ACIE68_000369"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFX5906745.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(15835..16704)
                     /gene="amiA"
                     /locus_tag="ACIE68_000370"
     CDS             complement(15835..16704)
                     /gene="amiA"
                     /locus_tag="ACIE68_000370"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFX5906746.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            16918..17343
                     /locus_tag="ACIE68_000371"
     CDS             16918..17343
                     /locus_tag="ACIE68_000371"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFX5906747.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            17330..17779
                     /locus_tag="ACIE68_000372"
     CDS             17330..17779
                     /locus_tag="ACIE68_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFX5906748.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            17840..18415
                     /locus_tag="ACIE68_000373"
     CDS             17840..18415
                     /locus_tag="ACIE68_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFX5906749.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            18511..19410
                     /gene="yfeX"
                     /locus_tag="ACIE68_000374"
     CDS             18511..19410
                     /gene="yfeX"
                     /locus_tag="ACIE68_000374"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFX5906750.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLVAL"
     gene            complement(19468..20772)
                     /gene="pbp4b"
                     /locus_tag="ACIE68_000375"
     CDS             complement(19468..20772)
                     /gene="pbp4b"
                     /locus_tag="ACIE68_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFX5906751.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(20777..22201)
                     /gene="murP"
                     /locus_tag="ACIE68_000376"
     CDS             complement(20777..22201)
                     /gene="murP"
                     /locus_tag="ACIE68_000376"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFX5906752.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(22205..23101)
                     /gene="murQ"
                     /locus_tag="ACIE68_000377"
     CDS             complement(22205..23101)
                     /gene="murQ"
                     /locus_tag="ACIE68_000377"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFX5906753.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            23265..24122
                     /gene="murR"
                     /locus_tag="ACIE68_000378"
     CDS             23265..24122
                     /gene="murR"
                     /locus_tag="ACIE68_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFX5906754.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            24251..25042
                     /gene="ucpA"
                     /locus_tag="ACIE68_000379"
     CDS             24251..25042
                     /gene="ucpA"
                     /locus_tag="ACIE68_000379"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFX5906755.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            25213..26229
                     /gene="cysP"
                     /locus_tag="ACIE68_000380"
     CDS             25213..26229
                     /gene="cysP"
                     /locus_tag="ACIE68_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFX5906756.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELGKLLA
                     AGRN"
     gene            26229..27062
                     /gene="cysT"
                     /locus_tag="ACIE68_000381"
     CDS             26229..27062
                     /gene="cysT"
                     /locus_tag="ACIE68_000381"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFX5906757.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLILLFSINTLQSRFGRRVVG
                     H"
     gene            27062..27937
                     /gene="cysW"
                     /locus_tag="ACIE68_000382"
     CDS             27062..27937
                     /gene="cysW"
                     /locus_tag="ACIE68_000382"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFX5906758.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLSNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            27927..29024
                     /gene="cysA"
                     /locus_tag="ACIE68_000383"
     CDS             27927..29024
                     /gene="cysA"
                     /locus_tag="ACIE68_000383"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFX5906759.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            29158..30069
                     /gene="cysM"
                     /locus_tag="ACIE68_000384"
     CDS             29158..30069
                     /gene="cysM"
                     /locus_tag="ACIE68_000384"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFX5906760.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(30072..30440)
                     /locus_tag="ACIE68_000385"
     CDS             complement(30072..30440)
                     /locus_tag="ACIE68_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFX5906761.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            30545..31396
                     /gene="pdxK"
                     /locus_tag="ACIE68_000386"
                     /gene_synonym="thiJ"
     CDS             30545..31396
                     /gene="pdxK"
                     /locus_tag="ACIE68_000386"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFX5906762.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(31438..31947)
                     /gene="crr"
                     /locus_tag="ACIE68_000387"
     CDS             complement(31438..31947)
                     /gene="crr"
                     /locus_tag="ACIE68_000387"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFX5906763.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(31988..33715)
                     /gene="ptsI"
                     /locus_tag="ACIE68_000388"
     CDS             complement(31988..33715)
                     /gene="ptsI"
                     /locus_tag="ACIE68_000388"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFX5906764.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(33760..34017)
                     /gene="ptsH"
                     /locus_tag="ACIE68_000389"
     CDS             complement(33760..34017)
                     /gene="ptsH"
                     /locus_tag="ACIE68_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFX5906765.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(34401..35372)
                     /gene="cysK"
                     /locus_tag="ACIE68_000390"
     CDS             complement(34401..35372)
                     /gene="cysK"
                     /locus_tag="ACIE68_000390"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFX5906766.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(35557..36318)
                     /gene="cysZ"
                     /locus_tag="ACIE68_000391"
     CDS             complement(35557..36318)
                     /gene="cysZ"
                     /locus_tag="ACIE68_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFX5906767.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            36548..37534
                     /gene="zipA"
                     /locus_tag="ACIE68_000392"
     CDS             36548..37534
                     /gene="zipA"
                     /locus_tag="ACIE68_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFX5906768.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            37605..39620
                     /gene="ligA"
                     /locus_tag="ACIE68_000393"
     CDS             37605..39620
                     /gene="ligA"
                     /locus_tag="ACIE68_000393"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFX5906769.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            39622..39840
                     /locus_tag="ACIE68_000394"
     CDS             39622..39840
                     /locus_tag="ACIE68_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFX5906770.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(39837..40835)
                     /locus_tag="ACIE68_000395"
     CDS             complement(39837..40835)
                     /locus_tag="ACIE68_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFX5906771.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            40925..41851
                     /locus_tag="ACIE68_000396"
     CDS             40925..41851
                     /locus_tag="ACIE68_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFX5906772.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVEHQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(41842..42183)
                     /locus_tag="ACIE68_000397"
     CDS             complement(41842..42183)
                     /locus_tag="ACIE68_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFX5906773.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQCLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(42304..42379)
                     /locus_tag="ACIE68_000398"
     tRNA            complement(42304..42379)
                     /locus_tag="ACIE68_000398"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42344..42346),aa:Lys,seq:ttt)
     gene            complement(42384..42459)
                     /locus_tag="ACIE68_000399"
     tRNA            complement(42384..42459)
                     /locus_tag="ACIE68_000399"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42424..42426),aa:Val,seq:tac)
     gene            42718..44133
                     /gene="gltX"
                     /locus_tag="ACIE68_000400"
     CDS             42718..44133
                     /gene="gltX"
                     /locus_tag="ACIE68_000400"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFX5906774.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(44185..44577)
                     /locus_tag="ACIE68_000401"
     CDS             complement(44185..44577)
                     /locus_tag="ACIE68_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFX5906775.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(44579..44938)
                     /locus_tag="ACIE68_000402"
     CDS             complement(44579..44938)
                     /locus_tag="ACIE68_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFX5906776.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            45159..45234
                     /locus_tag="ACIE68_000403"
     tRNA            45159..45234
                     /locus_tag="ACIE68_000403"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45192..45194,aa:Ala,seq:ggc)
     gene            45274..45349
                     /locus_tag="ACIE68_000404"
     tRNA            45274..45349
                     /locus_tag="ACIE68_000404"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45307..45309,aa:Ala,seq:ggc)
     gene            45557..47746
                     /locus_tag="ACIE68_000405"
     CDS             45557..47746
                     /locus_tag="ACIE68_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFX5906777.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQVLTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMINILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(47796..48998)
                     /gene="nupC"
                     /locus_tag="ACIE68_000406"
     CDS             complement(47796..48998)
                     /gene="nupC"
                     /locus_tag="ACIE68_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFX5906778.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            49334..50572
                     /locus_tag="ACIE68_000407"
     CDS             49334..50572
                     /locus_tag="ACIE68_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFX5906779.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(50713..51039)
                     /gene="ypeC"
                     /locus_tag="ACIE68_000408"
     CDS             complement(50713..51039)
                     /gene="ypeC"
                     /locus_tag="ACIE68_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFX5906780.1"
                     /translation="MFRSLFLAAALMAFTPLVANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(51154..52410)
                     /locus_tag="ACIE68_000409"
     CDS             complement(51154..52410)
                     /locus_tag="ACIE68_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFX5906781.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASALQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHSMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            52614..53579
                     /gene="glk"
                     /locus_tag="ACIE68_000410"
     CDS             52614..53579
                     /gene="glk"
                     /locus_tag="ACIE68_000410"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFX5906782.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHNNPGLLGSGAHLRQTLGHIL"
     gene            53798..54124
                     /locus_tag="ACIE68_000411"
     CDS             53798..54124
                     /locus_tag="ACIE68_000411"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFX5906783.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            54146..55393
                     /locus_tag="ACIE68_000412"
     CDS             54146..55393
                     /locus_tag="ACIE68_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFX5906784.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIVAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            55408..56493
                     /gene="ypdF"
                     /locus_tag="ACIE68_000413"
     CDS             55408..56493
                     /gene="ypdF"
                     /locus_tag="ACIE68_000413"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFX5906785.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            56493..57530
                     /gene="ypdE"
                     /locus_tag="ACIE68_000414"
     CDS             56493..57530
                     /gene="ypdE"
                     /locus_tag="ACIE68_000414"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFX5906786.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            57555..60050
                     /gene="ptsP"
                     /locus_tag="ACIE68_000415"
     CDS             57555..60050
                     /gene="ptsP"
                     /locus_tag="ACIE68_000415"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFX5906787.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(60053..60910)
                     /locus_tag="ACIE68_000416"
     CDS             complement(60053..60910)
                     /locus_tag="ACIE68_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFX5906788.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(60923..61657)
                     /gene="ypdB"
                     /locus_tag="ACIE68_000417"
     CDS             complement(60923..61657)
                     /gene="ypdB"
                     /locus_tag="ACIE68_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFX5906789.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(61672..63369)
                     /gene="ypdA"
                     /locus_tag="ACIE68_000418"
     CDS             complement(61672..63369)
                     /gene="ypdA"
                     /locus_tag="ACIE68_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFX5906790.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT        14877 a        16106 c        17551 g        14959 t
ORIGIN      
        1 atgaaagttt atgcaccaca gcgaatatct ctccattctt agtgatctac ctcacctttt
       61 aaacgcgctt gccgaatttt gttatttact ctgacgaaaa attgtcacga tacacgaaag
      121 tttttcacag gcggcgactc atggataaag aacgcatcat tcaggaattt gtgcccggca
      181 aacaggtcac actggcgcat ctcatcgcgc atcccggtga ggaactggcg aagaaaatcg
      241 gcgttccgga tgcgggcgca attggcatta tgacgctaac tcccggcgaa acggcgatga
      301 tcgccggtga tttagcgctg aaagccgctg atgtgcatat cggttttctc gaccgcttca
      361 gcggtgcatt ggtgatttat ggctcggtgg gcgcagtaga agaagcgtta tcacaaaccg
      421 tcagcggttt aggtcgctta ttaaattata cgctttgtga aatgaccaaa agttaatacg
      481 aggcattcat gaaacgaatt gcgtttgtgg gtagcgtcgg ggcaggaaaa acaacgttat
      541 ttaatgcgtt gcaggggaat tataccctcg ccagaaaaac acaggccgtg gaatttaatg
      601 ataagggcga tattgatacg ccgggtgaat attttagcca tccccgctgg tatcacgcct
      661 taattaccac gctacaggat gtagacatgc tgatctatgt ccatggcgca aatgacccgg
      721 aaagtcggtt gcctgccggg ttgctggata ttggcgtcag caaacggcaa atcgccgtca
      781 tcagtaaaac ggacatgcca gatgctgacg tcgccgcaac gcgaaagtta ttgcttgaga
      841 cgggtttcga agagccgatt ttcgagctaa acagccacga cccgcaaagc gtgcagcaac
      901 tggtggatta tctggcatct ctcaccaaac aggaggaagc aggtgaaaaa actcatcaca
      961 gcgaatgata ttcgtgaagc acacgcgcgc ggcgaacagg caatgtccgt ggttctgcgc
     1021 gccagcatta ttaccccgga agcccgtgaa gtggcggatc tactgggttt taccatcacc
     1081 gaatgtgatg aatcgatccc ggtaacggcg tccgtgcccg ccagcgtgcc agccgacaaa
     1141 accgaaagcc agcgcatccg cgaaaccatc attgcccagc tgccggaagg ccagtttacc
     1201 gaaagcctgg tcgcacagct gatggaaaaa gtgatgaagg aaaaacagtc gctggagcag
     1261 ggcgcaatgc agccgagctt caaatcggtg accggcaaag gcggcatcaa agtgattgat
     1321 ggcagcagcg tcaagtttgg tcgtttcgac ggcgcagagc cgcattgcgt gggcttgact
     1381 gatcttgtga ccggagacga cggcagcagc atggccgccg gttttatgca gtgggagaac
     1441 gcattcttcc cgtggaccct gaactacgac gaaatcgaca tggtgctgga aggggagcta
     1501 catgttcgcc acgaaggcga aaccatgatc gccaaagcag gcgacgtgat gtttatcccg
     1561 aaaggctcca gcatcgaatt tggtacgaca tccagcgtga aattcctgta tgtcgcctgg
     1621 ccggctaact ggcaatccct atgaaagatt tcatcaccga agcatggcta agagcgaacc
     1681 atacgctcag cgaaggcgca gagatccatc tccctgcgga cagtcgcctg acgccctctg
     1741 cccgggagtt gctggaaagc cgccatctgc gcatcaagtt tattgacgag cagggccgcc
     1801 tgtttgttga cgatgaacag cagcagccgc agcccgttca tgggctgacc agtagcgatg
     1861 aacatccgca ggcgtgctgc gaactgtgcc gccagccggt ggcaaaaaag ccggatacgc
     1921 tgactcacct gtcggcggaa aaaatggtcg ccaaaagcga tccgcgtctg ggttttcgcg
     1981 cggttctcga cagcaccatt gcgctggcag tgtggctgca aattgaactg gcggaaccgt
     2041 ggcagccgtg gctggcggat atccgttcgc gtcttggcaa cattatgcgc gccgatgcgc
     2101 tgggggaacc gctgggctgc caggcgattg tcgggctttc tgacgaagat ctgcatcggc
     2161 tttctcatca gccgctgcgc tatctcgacc acgatcatct ggtgccggaa gccagccacg
     2221 ggcgcgatgc tgcgttgctc aatctgctac gcaccaaagt gcgggaaacc gaaaccgtag
     2281 cggcgcaggt ctttatcacc cgcagctttg aagtattacg cccggacatt ttgcaggcgc
     2341 tgaaccgcct ttcgagcacg gtctacgtga tgatgatcct gagcgtaacg aagcagccgc
     2401 taacggtgaa gcagatccaa caacgactgg gggaaacgca atgattattg aacgttgtcg
     2461 tgaactggcg ttgcgagcgc ccgccagagt ggtttttccg gatgcgttag accaacgtgt
     2521 gctgaaagcc gcgcaatatt tacatcaaca aggtctggca acgcccattc tggtcgccaa
     2581 tccgtttgaa cttcgtcagt ttgcgctcag tcacggcgtg gcgatggacg ggctacaggt
     2641 gattgatccg catggcaacc tcgcaatgcg ggaagaattt gctcatcgct ggctggcccg
     2701 cgcgggcgaa aaaacgccgc cggatgcgct ggaaaaactt accgaccctc tgatgttcgc
     2761 cgcagcaatg gtcagcgccg gtaaagcgga tgtctgtatc gcgggcaacc tctcttccac
     2821 ggcaaatgtg ctgcgtgccg gattacgcat tatcggcttg cagccaggct gtaaaacgct
     2881 ctcttccatt ttcctgatgt tgccacagta cagcggcccg gcgttgggct ttgccgattg
     2941 cagcgtggtg ccacagccga cggcggcgca gctggcggat atcgcgcttg ccagtgccga
     3001 aacctggcgc gccatcaccg gagaagagcc gcgcgtggcg atgctgtcgt tttccagtaa
     3061 cggtagcgcc cgtcacccct gcgttgccaa cgtccagcag gcgacagaaa tcgtccgtga
     3121 gcgcgcacca aagctggtgg ttgatggcga gttacagttt gacgccgcct tcgtgccgga
     3181 agtggcggcg caaaaagcgc ctgccagccc gctacagggc aaggccaatg tgatggtttt
     3241 tccgtcgctg gaagccggaa atattggcta caaaatcgca caacgactcg gcggatatcg
     3301 tgccgtcggg ccattgatac aaggacttgc cgcgccgatg cacgatctct ctcgtggttg
     3361 tagcgtgcag gaaattatcg agctggcgct ggtggcagct gtgccgcgtc agacaaaagt
     3421 gaaccgcgaa agcagtttac aaacactggt tgaatgaacg gtcccgttct ggaccctgat
     3481 aaagaggaaa gcacaatgga agcattagga atgatcgaaa cccggggcct ggttgcactg
     3541 attgaggctt ctgacgcgat ggtgaaagct gcgcgcgtca agctggttgg tgttaagcag
     3601 attggcggcg gcctgtgtac tgccatggtt cgcggtgatg ttgcagcctg taaagcggcg
     3661 accgatgccg gtgccgcagc ggcacagcgt attggcgagc tggtatccgt acacgttatc
     3721 ccgcgtccgc acggtgacct ggaagaagtc ttcccgatcg gcctgaaagg cgatagcagc
     3781 aacctgtaat caccctgtgt gacgtcgatt cgggcgtcac gctctctcct ttttcatttt
     3841 accttctgcg gagggtaggg ggagctttta cctgaaatac gccacggagg cgggtatgaa
     3901 actggcagtc gtcactggac aaattgtttg taccgtacgc catcacggac tggcgcatga
     3961 caaattgctg atggtggaaa tgattgatcc acaaggtaat cctgacgggc aatgcgccgt
     4021 cgccatcgac aatattggcg cgggaaccgg ggagtgggtg ttactggtga gtggcagttc
     4081 cgcccgccag gcgcataaaa gcgaaacgtc accggtcgat ctgtgcgtga ttggcattgt
     4141 cgatgaggtg gtgtctggcg gtcaggtaat tttccacaaa taaggcagaa catcatgaat
     4201 caacaggata ttgaacaggt ggtgaaagcg gtactgctga aaatgcaaag cagtgacacg
     4261 ccgtccgccg ccgttcatga gatgggcgtt ttcgcgtccc tggatgacgc cgttgcggca
     4321 gccaaagtcg cccagcaagg gttaaaaagc gtggcaatgc gccagttagc cattgctgcc
     4381 attcgtgagg caggcgaaaa acacgccaga gatttagcgg aacttgccgt cagtgaaacc
     4441 ggcatgggcc gcgttgaaga taaatttgcc aaaaacgtcg ctcaggcgcg cggcacgcca
     4501 ggcgttgagt gtctctctcc gcaggtgctg accggcgaca acggcctgac tttaattgaa
     4561 aacgcgccct ggggcgtggt ggcttcggta actccgtcca ctaacccggc ggcaaccgta
     4621 attaataacg ccatcagcct gattgccgcg ggcaacagcg tcatttttgc cccgcatccg
     4681 gcggcgaaaa aagtctccca gcgggcgatt acgctgctca accaggcgat tgttgccgca
     4741 ggtgggccgg aaaacttact ggttactgtg gcaaatccgg atatcgaaac cgcgcaacgc
     4801 ttgttcaagt ttccgggtat cggcctgctg gtggtaaccg gcggcgaagc ggtagtagaa
     4861 gcggcgcgta aacacaccaa taaacgtctg attgccgcag gcgctggcaa cccgccggta
     4921 gtggtggatg aaaccgctga cctcgcccgt gctgctcagt ccatcgtcaa aggcgcgtct
     4981 ttcgataaca acatcatttg tgccgacgaa aaggtactga ttgttgttga tagcgtagcc
     5041 gatgaactga tgcgtctgat ggaaggccag cacgcggtga aactgaccgc agaacaggcg
     5101 cagcagctgc aaccggtgtt gctgaaaaat atcgacgagc gcggaaaagg caccgtcagc
     5161 cgtgactggg ttggtcgcga tgcggcgaaa atcgcggcgg caatcggcct gaacgtgccg
     5221 caagaaacgc gtttgctgtt tgtggaaacc actgcagaac atccgtttgc cgtgaccgaa
     5281 ctgatgatgc cggtgctgcc cgtcgtgcgc gtcgccaacg tggcggatgc cattgcgctg
     5341 gcggtgaaac tggaaggcgg ttgccaccac acggcggcaa tgcactcgcg caacatcgaa
     5401 aacatgaacc agatggcgaa tgctattgat accagcattt tcgttaagaa cggaccgtgc
     5461 attgccgggc tggggctggg cggggaaggc tggaccacca tgaccatcac cacgccaacc
     5521 ggtgaagggg taaccagcgc gcgtacgttt gtccgtctgc gtcgctgtgt attagtcgat
     5581 gcgtttcgca ttgtttaagg agtaacagat ggcgcacgac gaacaatggc tcaccccacg
     5641 tctgcaaacg gcggcaacgc tgtgtaacca gacgcccgcc gcgacggaat ctccgctgtg
     5701 gctgggggtg gatctgggca cctgcgatgt ggtgtcgatg gttgtcgacc gcgacggtca
     5761 gccggtggcg gtatgcctcg actgggccga tgtggtccgc gacggcatcg tctgggattt
     5821 cttcggcgct gtcaccattg ttcgtcgcca tctcgacacg ctcgagcagc aattcggtcg
     5881 ccgttttagc catgcggcga cttcatttcc tccaggtacg gacccgcgta tttccatcaa
     5941 cgtgctggag tctgccgggc tggaagtgag ccatgtgctg gatgagccaa ccgcagtcgc
     6001 ggatctactg caactggata acgcaggcgt agtggatatc ggcggtggca ctaccggcat
     6061 tgccatcgtg aaaaagggca aagtgacgta ctcggcggat gaagccaccg gcggacatca
     6121 catctctctg actctcgccg gaaatcgccg tatttcgctg gaagaggcgg agcagtacaa
     6181 gcgcggtcac ggtgacgaga tttggcctgc ggtgaaaccg gtgtacgaaa aaatggcgga
     6241 cattgtcgcc cggcatattg aagggcaggg cattactgat ttatggctgg cgggcggctc
     6301 ctgtatgcaa ccgggcgtgg cagaactgtt tcgcaaacaa ttcccggcgt tacaggtgca
     6361 tttaccgcag cacagtttgt ttatgacccc gctggcgatc gccagtagtg ggcgagagaa
     6421 agcggagggg ctatatgcaa aatgaattgc agaccgcgct ctttcaggcg ttcgataccc
     6481 tgaatctgca acgggtaaaa acatttagcg ttccaccggt gacgctttgc ggtctgggcg
     6541 cggtgagcag ttgcgggcag caagcgcaaa cgcgtgggct gaaacatctg ttcgtgatgg
     6601 cagacagctt tttgcatcag gcagggatga ccgccgggct gacgcgcagc ctggccgtta
     6661 aaggcatcgc catgacgctc tggctatgtc cggtgggcga accgtgcatt accgacgtgt
     6721 gtgcagccgt ggcgcagttg cgtgagtcag gttgtgacgg ggtgatcgca tttggcggcg
     6781 gctcggtgct ggatgcggcg aaagccgtgg cgttgctggt gacgaacccg gatagcacgc
     6841 tggcagagat gtcagaaacc agcgttctgc aaccgcgctt gccgctgatt gccattccaa
     6901 ctaccgccgg aaccggctct gaaaccacca atgtaacggt gattatcgac gcggtgagcg
     6961 ggcgcaagca ggtgttagcc catgcctcgc tgatgccgga tgtggcgatc ctcgacgccg
     7021 cattgaccga aggtgtgccg tcgcatgtca cggcgatgac cggcattgat gcgttaaccc
     7081 atgccattga agcatacagc gccctgaacg ctacaccgtt taccgacagt ctggcgattg
     7141 gtgccattgc gatgattggc aaatcgctgc cgaaagcggt gggctacggt cacgaccttg
     7201 ccgcgcgcga gagcatgttg ctggcttcat gtatggcggg aatggcgttt tccagtgcgg
     7261 gtcttgggtt gtgccacgcg atggcgcatc agccgggcgc ggcgctgcat attccacacg
     7321 gtctggcgaa cgccatgttg ctgccaacgg tgatggaatt taaccggatg gtttgtcgcg
     7381 aacggtttag tcagattggt cgggcactgc gaactaaaaa atccgacgat cgtgacgcta
     7441 ttaacgcggt aagtgagctg attgcggaag ttgggattgg taaacgactg ggcgatgttg
     7501 gcgcgacatc tgcgcattac ggtgcatggg cgcaggccgc gcaggaagat atttgtctgc
     7561 gcagcaaccc gcgtaccgcc agcctggaac agattgtcgg actgtacgcg gcggcgcaat
     7621 aaatgccgga tgcgacgctt gccgcgtctt atccggccta cgggattgca catgtagggc
     7681 ggataaggcg tttacgccgc atccgccaat aaataacaac taacagggag taaaggcgat
     7741 gggaattaac gaaatcatca tgtacatcat gatgttcttt atgctgatag ctgccgtaga
     7801 caggatcctg tcgcagttcg gcggttctgc tcgtttcctc ggtaagttcg gtaaaagtat
     7861 cgaaggatca ggcggtcagt tcgaagaagg ctttatggca atgggcgctc tgggtctggc
     7921 gatggtcggt atgaccgcgc tggcaccggt actggctcac gtactcgggc cggtaattat
     7981 cccggtttac gaaatgctcg gcgcaaaccc gtcaatgttc gccggaacac tgctggcgtg
     8041 cgatatgggc ggcttcttcc tcgccaaaga gctggcgggc ggcgacgtag cagcgtggct
     8101 atactctggg ttaattctcg ggtcgatgat ggggccaacg attgtgtttt ccattccggt
     8161 ggcgctcggc attatcgaac cttctgatcg tcgttatctg gcgctcggcg tgctggcggg
     8221 cattgtgacc attccgattg gctgtattgc tggtggtctg gttgctatgt actccggtgt
     8281 gcagatcaac ggtcagccgg tggaattcac tttcgccctg atcctgatga acatgatccc
     8341 ggtgatcatt gtcgcgattc tggtggcgct ggggctgaaa ttcatcccgg aaaaaatgat
     8401 caacggcttc cagatctttg ccaaattcct cgttgcattg atcaccctcg gtcttgccgc
     8461 tgcggtagtg aaattcctcc ttggctggga actgatcccc ggactggatc ctatctttat
     8521 ggcccctggc gataaacccg gtgaagtgat gcgcgccatt gaagttatcg gttctatctc
     8581 ctgcgtgctg ttaggggcgt atccaatggt gctgctgctg actcgctggt ttgaaaaacc
     8641 gctgatgagc gtcggtaaag tactgaatat gaacaacatc gcggcagccg gcatggtggc
     8701 aacgcttgcc aacaacatcc cgatgttcgg catgatgaag cagatggata cccgcggcaa
     8761 agtcatcaac tgcgccttcg ccgtttccgc tgctttcgcc ctgggcgacc acttaggctt
     8821 cgccgctgcc aacatgaacg ccatgatctt cccgatgatt gtcggcaagt tgatcggcgg
     8881 cgtaacggcg attggcgtgg cgatgatgct ggtgccaaaa gaagacgcga ccgcgactaa
     8941 aaccgaagcg gaggcacaat cgtgaacact cgccagctat tgagcgtcgg tatcgatatc
     9001 ggcaccacca ccacccaggt gattttctcc cacctggaac tggttaaccg tgcggcggtg
     9061 tcgcaggtgc cgcgctacga attcattaaa cgcgaaatta gctggcaaag cccggtattc
     9121 tttacccctg tcgataaaca gggtggttta aaagaagcgg aactgaaatc gctgatttta
     9181 gagcaatatc aggctgcggg cattgcgccg gaaagcgttg attctggtgc catcatcatc
     9241 accggtgaaa gcgcgaaaac ccgcaatgct cgcccggcgg tgatggcgct ctctcagtcg
     9301 ctgggcgatt ttgtcgttgc cagcgccggg ccgcacctcg aatccgtgat cgccggtcac
     9361 ggagccgggg cacaaaccct ttctgaacaa cggctgtgtc gggtactgaa tatcgacatc
     9421 ggcggtggca ccgcgaacta cgccctgttc gatgccggaa aaatcagcgg cactgcctgt
     9481 ctcaacgtcg gtggtcgcct gctggaaacc gacagccagg ggcgcgtggt ttacgctcat
     9541 aaaccggggc agatgattgt ggatgagtgc ttcggtgcag gcactgacgc ccgttcgctg
     9601 accggcgcgc agctggtgca ggttacccgg cggatggcgg cgctgattgt cgaagtgatt
     9661 gacggaacgc tttcgccgct cgcgcaggca ttgatgcaaa ccggtttgct gcccgcaggt
     9721 gttacgcccg aaatcattac gctttctgga ggcgtgggcg aatgttatcg ccaccagccc
     9781 gccgacccgt tctgttttgc cgatattggc ccacttctgg caacggcgct gcatgaccat
     9841 ccgcgcctgc gtgagatgaa tgtgcagttt ccggcgcaaa ccgtacgcgc cacggtgatt
     9901 ggcgcgggtg cacataccct ttcgctctct ggcagcacaa tctggctgga gggcgtacaa
     9961 ctgccgctgc gcaatttgcc ggtggcgatc ccgattaatg aaacggatct ggtgagtgcc
    10021 tggcaacagg cgctgcttca gctggatctt gatcccaaaa ctgacgcgta cgtgctggcg
    10081 cttcccgcct cgctgcctgt gcgttacgcc gcggtactga cggtcatcaa cgcgctggtc
    10141 gatttcgtcg cgcgttttcc gaatccgcat cccctgctgg tggtggccgg gcaggacttt
    10201 ggtaaagctc tgggcatgtt gttgcgccca cagctacaac aactcccgtt ggcagtcatt
    10261 gacgaagtga ttgtccgcgc gggggactat atcgacattg gtacgcctct ttttggcgga
    10321 tcggttgtgc cggtgacggt gaaatcactc gcatttcctt cctgagggaa cgacttatga
    10381 aactaaagac cacattgttc agcaatgtat atcagtttaa ggatgtaaaa gaggtgctgg
    10441 ctaaagccaa cgaactgcgt tcgggggatg tgctggcagg cgttgcagcg gcaagttcac
    10501 aggagcgcgt ggcggcaaag caggtgttgt cggaaatgac cgtagcggac atccgcaata
    10561 atccggtgat tgcctatgaa gatgactgcg tgacgcggct gattcaggac gatgttaacg
    10621 aaacggccta caaccagatt aaaaactgga gcatcagcga actgcgtgag tatgtgctga
    10681 gcgatgaaac cagcgtggac gacattgcct ttacccgcaa agggctgacc tcggaagtgg
    10741 tcgcggcggt agcgaagatt tgctccaacg cggacctgat ctacggcgcg aagaaaatgc
    10801 cggtaatcaa aaaggccaat accaccattg gtattccggg caccttcagc gcccgtttgc
    10861 agccaaatga cacccgtgac gacgtgcaaa gtatcgccgc gcaaatctac gaagggcttt
    10921 ccttcggggt gggcgatgcg gtgatcggcg ttaacccggt gactgacgac gtggaaaact
    10981 taagccgcgt gttggacacc atttacggcg tgatcgacaa attcaacatc ccaactcagg
    11041 gctgcgtact ggcgcacgtc accacccaga tcgaagcgat tcgtcgcggc gcgcctggcg
    11101 gactgatttt ccagagtatc tgtggcagcg aaaaagggct gaaagagttt ggcgtggaac
    11161 tggcgatgct cgacgaagcg cgcgcagtgg gtgcagagtt caaccgtatc gccggggaaa
    11221 actgcctcta cttcgaaacc ggacaaggct ctgcgctatc cgctggcgct aacttcggcg
    11281 ctgaccaggt gacgatggaa gcacgtaact atgggctggc gcgtcattac gatccgttta
    11341 tcgtcaacac cgtggtcggc tttattgggc cggagtatct ctacaacgac cgccagatta
    11401 tccgtgctgg cttagaagat cactttatgg gcaagctgag cggcatctct atgggctgtg
    11461 actgctgtta caccaaccac gctgacgctg accagaacct caacgaaaac ctgatgatcc
    11521 tgctcgccac cgcaggctgc aactacatca tggggatgcc gctgggtgat gacatcatgc
    11581 tcaactacca gaccaccgca ttccacgata ccgccactgt gcgtcagtta ctcaacctgc
    11641 gtccgtcacc ggagtttgaa cgctggctgg aaagcatggg cattatggca aacggtcgcc
    11701 tgaccaaacg ggcgggcgat ccgtcactgt tcttctgatg acgcggagat aacacatcat
    11761 ggatcaaaaa cagattgaag aaattgtacg cagcgtgatg gcgtcaatgg gacaaacggc
    11821 ccccgcgccg tcagaagcaa agtgcgccac caccaactgt gcggcaccgg tgacctcgga
    11881 aagctgtgcg ctggatttag gttccgctga agcaaaagcg tggattggtg ttgaaaaccc
    11941 gcatcgcgca gacgtattaa cagaactgcg ccgcagcacc gtggcccgcg tttgtaccgg
    12001 tcgtgccggt ccgcgtccac gtacccaggc gctgctgcgt ttcctggccg atcactctcg
    12061 ttcgaaagat accgtactga aagaagtgcc ggaagagtgg gtgaaagcgc agggcttgct
    12121 ggaagtacgc tctgaaatta gcgacaaaaa tctctacctg actcgcccgg atatgggccg
    12181 ccgcctgtgt gcagaagctg ttgaagcgct gaaagcacag tgtgttgcta atccggacgt
    12241 acaggtcgtt atttctgatg gcctgtcaac tgatgccatc accgtgaact acgaagagat
    12301 cctgccgccg ctgatggcgg gcctgaaaca ggccgggctg aaagtcggca cgccattctt
    12361 tgttcgttat ggtcgcgtga agattgaaga tcagatcggt gagatcctcg gcgcgaaagt
    12421 ggtgatcctg ctggtggggg aacgtccggg gttagggcag tcagaaagcc tctcctgcta
    12481 cgccgtttac tcgccgcgta tggcgaccac cgttgaggcc gatcgcacct gtatctctaa
    12541 cattcaccag ggcggcacgc cgccggttga agccgcagcc gtcattgtgg atttggcgaa
    12601 acgtatgctg gagcagaaag catccggcat caacatgacc cgataaggag gcatcatgcc
    12661 agctttagat ttgattcgac cgtcggtaac cgccatgcgg gtgattgcct ccgttaacgc
    12721 tgaatttgcg cgtgaactaa aattgccgcc gcatattcgt agcctcgggc tgatttctgc
    12781 tgattccgat gacgtcacct acattgccgc tgatgaagcg accaagcagg cgatggtgga
    12841 agtggtatat ggtcgctcgc tgtatgcggg cgctgcacac ggcccgtcac cgactgccgg
    12901 tgaagtgctg attatgcttg gtgggccaaa cccggcggaa gtgcgtgccg gtctggatgc
    12961 gatggttgca catattgaaa atggtgcagc attccagtgg gccaatgacg cagaaaacac
    13021 cgccttcctg gcgcatgtgg tttcgcgtac cggttcttat ctctcatcaa ccgccgggat
    13081 cacgcttggc gatccgatgg cgtatctggt ggcaccgccg ctggaagcga cctatggcat
    13141 tgatgcagcg ttgaaatatg ccgacgtaca gctggtgacc tatgtcccgc caccgtctga
    13201 aaccaactac tcggcagcat ttttaaccgg tagccaggcc gcgtgtaaag cagcctgtaa
    13261 cgcctttacc gatgcagtgc tggaaatcgc gcgtaatcca atccagcgtg cgtaacggag
    13321 gttgccgatg atcaatgcac tgggattgct ggaagtggac ggcatggtcg ccgcgataga
    13381 tgcagcggat gccatgctca aagcagctaa cgttcgtctg ctcagtcacg aagtgcttga
    13441 ccctggtcgc ttaacgctgg tggtggaagg cgatctggcg gcgtgtcgtg cggcgctgga
    13501 cgcaggttgt gctgccgcga tgcgtaccgg gcgtgtcatc agccgcaagg agatcggtcg
    13561 gccagacgat gacacccagt ggctggttac tggctttaac cgccagccga agcaacccgt
    13621 aagggaaccc gacgcgccag ttatcgtcgc ggaatctgct gacgagttgt tggcgctgtt
    13681 aacatcagta cgtcagggaa tgacggcagg agaagtggct gcccactttg gctggccgct
    13741 ggaaaaagcc agaaatgcgc tcgaacagct cttttctgcc gggacgttac gtaaacgcag
    13801 tagtcgttat cgtctcaagc cccattaacc tgtcggaggt gccgggtgtc ctacaacacc
    13861 cggcattaac atcatgaaaa agacccgtac agccaatttg caccatcttt atcatgaacc
    13921 cttacccgaa aacctgaagc tcacgccgaa ggtcgaagtg gataatgttc atcaacgaca
    13981 gacaacggat gtctatgaac atgctttaac gattaccgcc tggcagcaga tttacgatca
    14041 gctgcatccg ggcaagtttc atggtgaatt tacggaaatt ctactcgatg atattcaggt
    14101 ttttcgtgaa tacaccggtc tggcgctgcg tcagtcgtgc ctggtctggc cgaactcgtt
    14161 ctggtttggc attccggcga cgcgcggtga gcagggattt atcggttcgc aatgtctggg
    14221 aagcgcggaa atcgccaccc gccctggtgg cactgaattt gaactgagca cgccggatga
    14281 ttacacgatc ctgggcgtgg tgctttctga agatgtcatc acccggcagg ctaacttttt
    14341 gcataacccg gatcgggtat tacatatgct gcgtagccag tcggcgctgg aagtgaaaga
    14401 gcagcataaa gccgcgctgt ggggctttgt ccaacaggcg ctggcgacgt tttgcgagaa
    14461 cccggaaaat ctccatcagc ctgcagtgcg aaaagtgctg ggggataatt tgctaatggc
    14521 gatgggggcg atgctggaag acgcgcaacc aatggtgacg gcggaaagca tcagtcatca
    14581 gagttaccgt agattacttt cccgcgcccg tgaatatgtg ctggaaaata tgtctgagcc
    14641 ggtgacggtg ctggacttgt gtaatcaact gcatgtcagt cgccgcacgc tacaaaacgc
    14701 gtttcacgct attttaggca ttggcccaaa cgcgtggctg aaacgcattc gcctgaacgc
    14761 cgtacgccgc gaactgataa gcccgtggtc gcaaagcaca acggtaaaag acgccgccat
    14821 gcagtgggga ttctggcatc tggggcaatt tgccacggat taccagcagc tgtttgccga
    14881 gaagccgtcg ttgacgttgc atcagcggat gcgggaatgg gggtgaggga gttacaccca
    14941 atccctgacc ttaataaact cacttaacgc cgcttctggg ctgccatctt ttggctgata
    15001 atcatactcc cagcgtacca gtggcggcat tgacatcagg atcgactcgg tgcgcccgcc
    15061 agtttgcagg ccaaacagag tgccgcgatc ccagaccaga ttgaactcga cataacgacc
    15121 gcgacggtag agctgaaaat tgcgctcgcg ctcgccgtag gccatcgctt tacgtcgctc
    15181 tacaattggt aaataagcgt cggtgtagcc tttgcctacc gcctgcataa aggcaaaaca
    15241 gtggtcgaaa tctggcgtgt tcagatcatc aaagaacagc ccgccaatac cgcgctgttc
    15301 gttgcgatgt ttgaggtaga agtaatcgtc gcaccacttt ttgtaacggg gataaacgtc
    15361 ttcaccaaat ggcaggcaca ggtcacgggc ggtgcggtgc cagtgaatgg cgtcttcttc
    15421 aaaaccatag aaaggggtta aatcgaagcc gccgccaaac caccagacgg gctctgcacc
    15481 cggtttttcg gcaataaaaa aacgcacatt cgcgtggctg gtgggaacat acgggttatg
    15541 cggatgcact accagtgaaa cgcccatcgc ctcgaaactg cgcccggcaa gttccgggcg
    15601 atgagcggtg gcggaagctg gcatcgcctc gccgtggacg tgagaaaagt tgacgcctgc
    15661 ctgttcgaaa acaccaccat tacgcaacac ccgactacgc ccgccgccgc cagcttcgcg
    15721 ctgccaacta tcttcgacaa attctgcgcc atcgacggcg gtcagctgct gacaaatcgt
    15781 atcctgaagg ttgagcagaa actgtttaac ctggtgtgcg tcgggtttca taacttatcg
    15841 ctttttcgaa tgtgctttct ggttgtcgaa ccagtggaaa taactgatca cgccttcagc
    15901 aatcgctgtg gcgattttct gacgaaacgc cgccgtgcct aacagccgct cttcttccgg
    15961 gttggtgata aacgaggttt ccaccagcac cgaaggaacc gacggcgatt tcaacaccac
    16021 aaacgcggct tgttcggtgt tgcggctgtg cagtttatgc accggcttaa tctttttcag
    16081 aatatgcgag ccgagcgtca ggctgttttt aatagtatcg gtttgcacca gatcaaacag
    16141 cacttgctgc aataggtgat ccttgtcagt cgccttttta ccggcaactt catcggcgcg
    16201 gttttcgcgt tcagacaggt atttcgccat tgcgctactt gccccacggt tagagagggc
    16261 aaataccgaa gcaccggcag ctttcgggtt ggtaaagcca tcggcatgaa ttgacataaa
    16321 cagatctgcg ccatgtttat gggcgatttc aacgcgatcg taaagtggga taaacgtatc
    16381 gccagaacgc gttaaacgcg catcaatccc atgattacgc aaaatggaac ggacgttttt
    16441 agcaatcgcc agcaccacat gtttttcttt cgaaccgttg cgtccgatcg ctccggtatc
    16501 aattccgccg tggcctggat cgagaacaac gacacgtttg ccgccagatt ttttggcttt
    16561 cggcttgctg tgtccgttgc tggtttttaa aggttcgtct ttggcgatgg cttgcgacat
    16621 tcctgacaac gtcagggcag ccaaaccggc tttcagcacc tggcggcgcg aagtgagtgt
    16681 ttttagtggt ttaaaagtgc tcatacggcc tgagttgtaa taataaagtt ccagatgtta
    16741 tatcgtatcg cgtaatccgt tacgacggtt tgatgttgag aattgtttta cttttcattt
    16801 caatagttga caactcccca ttatgccact ttttcaaccg attttcgtca gatattggct
    16861 aattcgaccg ttcacgccat aatcagcctt ttaacccgca aaaaggtaac gaataccatg
    16921 gagatacgcg tatttcgcca ggaagatttc gaagaggtca tcaccctttg ggagcgttgc
    16981 gacttgctgc gtccgtggaa cgatccggaa atggacatcg agcgtaagat gaaccatgac
    17041 gtcagtttgt ttttggtcgc tgaggtaaac ggtgaagtgg tcggaacggt gatgggcggt
    17101 tatgacgggc atcgcgggtc tgcttattat cttggcgtgc atccagagtt tcgtgggcgt
    17161 gggattgcca atgcgttgct taatcggctg gagaaaaagc tgattgctcg tggctgcccg
    17221 aaaattcaga tcaatgtgcc ggaagataac gacatggtgc tcggaatgta tgaacgcctg
    17281 ggatatgaac acgccgacgt gctgagtctg ggtaagcgtt tgattgaaga tgaagagtac
    17341 tgagttccat cctgtccatt atgatgcgca cggacgcctg cgtttaccct tgctcttctg
    17401 gcttgtgcta ttgcttcagg cgcgaacctg ggtgctgttt gtcattgccg gtgcgtcgcg
    17461 tgagcagggt actgcgctgc taaatctgtt ttatcccgat cacgataatt tctggctggg
    17521 gctgattcct ggcattccgg cggtgctggc gtttttgttg agcggtcggc gggctacgtt
    17581 tcctcgcacc tggcgtgtac tctattttct gttgctgttg gcgcaggtgg ttttactttg
    17641 ctggcaaccg tggctgtggc tgaacggtga atccgttagc ggtatcggtc tggcgctggt
    17701 agtggcggat attgtggcgt taatctggct gctgaccaat cgacgtttac gcgcttgttt
    17761 taatgaagta aaagaataac ggcacttttt ggtgaatttg cactccaagc aacgttattg
    17821 aataaccaaa ggaagtgaca tgaaatcgct gcgtttaatg ttatgcgcta tgccattgat
    17881 gctgaccggc tgttccacga tgtcgtcagt taactggtct gccgctaacc cgtggaactg
    17941 gtttgggtcg tccaccaaag tgagcgagca gggcgttggt gaattaacgg cgtccacacc
    18001 actgcaagaa caagccattg ccgatgcgct tgatggcgat tatcgcctgc gcagcggaat
    18061 gaaaaccgcg aacggcaacg tggtgcgctt ttttgaagtg atgaagggcg acaacgtggc
    18121 gatggtgatt aacggcgatc aaggcacgat cagtcgcatt gacgtgctgg atagcgatat
    18181 tcctgctgac accggtgtta aaatcggtac accgtttagc gacctttaca gcaaagcatt
    18241 tggcaattgc caaaaagctg atggtgatga taatcgtgcc gtcgaatgta aagccgaagg
    18301 cagtcaacat attagctacc agttcagcgg ggaatggagt ggtcctgaag ggttaatgcc
    18361 ttcggacgat accctgaaaa actggaaagt cagtaaaatt atctggcggc gttaatttgc
    18421 gtctgaacaa accgccgccg cgaaaaaaca cgtaaaatag cgctcaacaa tgccacggat
    18481 tgcgtggcat tattattttc aggaggaaca atgtctcagg ttcagagtgg cattttgcca
    18541 gaacattgcc gcgcggcgat ttggatcgaa gccaacgtga aaggggaagt tgacgccctg
    18601 cgtgcggcca gtaaaacatt tgccgacaaa ctggcaactt ttgaagcgaa attcccggac
    18661 gcgcatcttg gtgcggtggt tgcctttggt aacaacacct ggcgcgctct gagcggcggc
    18721 gttggggcag aagagctgaa agattttccg ggctacggta aaggccttgc gccgaccacc
    18781 cagttcgatg tgttgatcca cattctttct ctgcgtcacg acgtaaactt ctctgtcgcc
    18841 caggcggcga tggaagcctt tggtgactgc attgaagtga aagaagagat ccacggcttc
    18901 cgttgggttg aagagcgtga cctgagcggc tttgttgacg gtacggaaaa cccggcgggt
    18961 gaagagacgc gtcgcgaagt ggcggttatc aaagacggcg tggatgcggg cggcagctat
    19021 gtgtttgtcc agcgttggga acacaacctg aagcagctca accggatgag cgttcacgat
    19081 caggagatga tgatcgggcg caccaaagag gccaacgaag agatcgacgg cgacgaacgt
    19141 ccggaaacct ctcacctcac ccgcgttgat ctgaaagaag atggcaaagg gctgaagatt
    19201 gttcgccaga gcctgccgta cggcactgcc agtggcactc acggtctgta cttctgcgcc
    19261 tactgcgcgc gtctgcataa cattgagcag caactgctga gcatgtttgg cgataccgat
    19321 ggtaagcgtg atgcgatgtt gcgtttcacc aaaccggtaa ccggcggcta ttatttcgca
    19381 ccgtcgctgg acaagttggt ggcgctgtaa gcgtttctga ttcattgatt aaaggccagg
    19441 cagtaacatt actgactggc ctttttatta cttttgcttt aacgccgcat acacctgatc
    19501 aactacccaa ccatacgttg caatcggcag ctgaccgctt tcgaacatat tgggattctt
    19561 ttgcggatcg gcaaccggcg aatgtggctt gttgcttaac atcacaatcg ccatatgatt
    19621 caccggatcg ataacggtca ccgttccggt ccagccagtg tgaccgtagg tttgcgggct
    19681 tgccagcgtg ccaaacgtcg gcgtcatggt ggcattacca ttcacgcgcc agccgaggcc
    19741 aaaagtggca tcttccttag agctggtggt gaacatcttc accgtttccg cactgaacag
    19801 ctgcacatcg ccatagcccc cgccgttcag catcgtttgc attaacaccg caatatcgcc
    19861 ggtattggaa aacaaacctg cgtgcccgga aacgccgccc atcgaataaa aggctttttc
    19921 atcgtgcacc tgaccccaga gagtggaggt gcggatattc gggaaatgga tcacgccatc
    19981 gcgggtattg ccgttcaatt ccgtggcggc aatttgttgc ggtttaaagc ctttcagcag
    20041 cgggttaaat accgtatgtg tcaggccgag cgggcgataa atcgactctt caacatagcg
    20101 gtcaagcggc tgaccggtga ccgactcgac gataaatcca agcagcatat aatcgacatc
    20161 actgtaaata tgcttgctgc cgggctgata ctctagcggc gtgcgcttga tcatctccag
    20221 cgtctggcct ttatcctggg aatataacgc gcccgcgacc gctttattcg ggtattgcgg
    20281 atctgccggg aaaccgccgc tgtgatgcag caggtcagaa atccgcaggg tgtttttgcc
    20341 tttaatggtg tcatttggac tatcggcaaa ccccggaata tatttcgcaa tccgatcgtc
    20401 aggatgcagt ttgccttcgc tcatcagctt ttgcagggcg aagttcgtgg cgtacatttt
    20461 ggtgtttgag gccaggtcat acagcgtccc ggtggtggct ttgacaggct gttccatcaa
    20521 cacgctgcca tcgtactttt tcgccgctcc ccaggctttg cgatacacaa tctgattatc
    20581 tttaatgatc agcaggttta cgccgggata accggcgtcc acttgctggc taatccagcg
    20641 atccatctgg ttaagccgtt cgacgttgaa ccctgctttc tctggcgaac tttctgtcag
    20701 aacagggtat tttgcggcgc tgacgctaca actaacagcc agcagagaaa gatagagcat
    20761 tgtccgtttc atgatatcag tccagattga cgttacggca gccaaagagc gtggtgaaaa
    20821 taaacccgca aacccatgcc accagaatcc cgccagcata aaccgccatt gccggaagga
    20881 tgccttgtgc ggaagtcatc agcggcagcg ccaccagacc agacggccca aaggcgctgt
    20941 ttaagcccat cggcagaccc caccaggcta tcaggccgat aaacaaaccg cccgctgcgc
    21001 cgcctaaaca ggcggtaata aacggtttca tgcggggcag ggtgacgccg taaatcaacg
    21061 gttcaccaac gcccagcaga ccgggaataa tcgccccgcg tacctgactg cgtaatgcac
    21121 tgtgcggttg cgcccgccag tagagtgcca gcgccgcgcc cacctggccc gcgcctgcca
    21181 ttgaaaggat gggaaataag ctgttgaatc cctggctgtc cattaacgcg aggtaaacag
    21241 gaataaagcc ctgatgcacg ccaaatacca cggcgatcag gaacagcccg gctaaaaccg
    21301 cacaaccgaa cggattactg ttcaggtgca taaacagcca tgacatacct tcgaataacc
    21361 agccacccag cggcataatg atcaggtacg caagcgtagc ggtgatcagc agggtgatta
    21421 acgaggtcag cagcatgtcg agatcgtccg gcataaagcg gcgcaccatg ccttcaatgc
    21481 gggcgcaggc ccacgcggca atcagcacgc cgataatatt gccgcgcgga tcgatgggca
    21541 gaccaaagaa atcgtgaaaa ccggcgtagt aaccggtggt tgcagcgggg ttataaccga
    21601 gcaaaaagag tgcggcaata attgcgccat tgacgcccgt gccgccaaat gcctgggcgg
    21661 cgttatagcc caccagaatc accaggaaag tgaacaaacc tttgctgaac accttcataa
    21721 aattcagcgc gtcgggtagc gtgccctgag catctgccgg aacgtgcatc accgtggcaa
    21781 ttaacgtcgc tatccccagc agcagaccgg cggcaataaa accggggatc agcggcgtga
    21841 agatggtggc gaatttggcg agaaattgtt gcacgccgga ggtttgtttg gcttttaact
    21901 gacgtttgtt ctgggcggcg atttctgcgg catcctgtac cggtgcctcg cccagcagct
    21961 cgctcatcgc ttttgcggca cgatgcgctt taccaggccc aaaaacgacc tgcacctggt
    22021 cgctggtcaa aatgacgccc ttcacgcctt ccagcgtttt gatattggga tcaaccagtg
    22081 aactgtcatg tacacccaga cgcaggcgcg tcatacagtt accacaactg gcgatatttc
    22141 ccggtccgcc gacacgggta agaatggtgt tcagaagttc actgctgatc tctttggcca
    22201 tgggttattc cttgtctaaa acctgacgaa taaagccgcc gtgttgatcc aggcgttttt
    22261 tagcttctgc ggcatcgaga tttttcagca ccatcacaat ggccgtttta cagttgcgct
    22321 cgcaggcaat taacgccgct tccgcttgtt cggcattaca tccggtggcg tttttaacga
    22381 tattgacctg gcgcacatgc agtttttcgt tggtggcgac cacatcgacc atcaggttgc
    22441 cgaacacttt gccagattta atcatcagcc cggtggaaag catattgagc accagtttct
    22501 gcgctgtgcc tgctttcatt cgcgaagaac cagtgaccac ttcggcacct acaatcggtg
    22561 taatggcaaa ctcggcggtg gtggaaacgg cactccccgg attacaggaa attcccactg
    22621 tgcggcagcc gagctggcgt gcgtattcca gtccggcaat cacatacggc gtgcgaccgc
    22681 tggcagcaat gccaaccacc acatcctgtg ccgttaaatt aatatttttc agatcgttaa
    22741 caccaccttc ccggctatct tcagcgcctt ccaccgcgtg ctgaatggca tattcgcctc
    22801 cagcaatcaa accaaccacc agacccggtt tcacgccgta ggtgggcgga cattcgctgg
    22861 catccagaat ccccagacga ccggatgttc ccgcaccgag ataaatcaga cgaccgccgc
    22921 cgctgacctg ggcgtggata acatcgatcg ccgcggcgat atccggcagg acgcgctcaa
    22981 cggcaagcgg tacggtttta tcttcatcgt tgataatccg gcacatttcc agcgtcgata
    23041 cgcggtcaat ttcagccgag gcggtgttcg agccttcagt aatcatcttt tctagttgca
    23101 tcggtggtga ccttactatt tacgatcaaa taattgcaga tgtatggaat catatatccc
    23161 gaattgacag acttaaagga atcattgagt ctacactcgc agagtgtgaa gagcgtcata
    23221 agaacttaag cggaatttat gctgccagcc caggagagtg gaagatgttg tacctgacga
    23281 aaataagcaa tgcggggagt gaattcaccg aaaacgagca aaaaatcgct gactttctgc
    23341 aggccaacgt gagtgagctg caatccgttt cttcacggca gatggcgaaa cagctcggca
    23401 ttagccagtc gagcatagtg aaatttgccc aaaaactcgg cgcgcaagga tttactgaat
    23461 tgcgtatggc gttaataggc gaatacagtg ccagccggga gaaaacgaat gccacggcgc
    23521 tgcacctgca cagttcgatt accagtgatg attcgctgga agtgattgcc cgcaaattga
    23581 atcgcgaaaa agagctggcg ctggagcaaa cctgtgcgtt gttcgactac gcgcggctgc
    23641 aaaaaatcat tgaggtcatc agtaaagcac cgtttatcca gataacgggc ctgggcggat
    23701 cggcgctggt ggggcgcgat ctgtcgttca aactgatgaa aattggctat cgcgtggcct
    23761 gcgaagccga cacgcacgta caagccaccg tttcccaggc gttgaaaaaa ggcgatgtac
    23821 aaatcgctat ctcttacagc ggcagtaaaa aagagattgt cttatgcgca gaagcggcac
    23881 gaaagcaggg ggcaacggtg attgccatta cttctctggc tgattctccg ctgcggcggc
    23941 tggcgcattt cacgctcgat accgtttccg gtgaaaccga atggcgtagc tcgtcgatgt
    24001 ctacccgcac ggcgcaaaat tctgttaccg atttactgtt tgttggtctt gtgcaactca
    24061 atgatgtgga gtcattgaaa atgattcagc gcagcagtga gctaacacaa cgcctgaaat
    24121 gatgcataaa gcagcgactg gattgagatt ttcctgaatt agtgagctga tccgcagcaa
    24181 tattttgttt atcctgtatt ttcagaggga atggagtgta acgctctgta ttaacaagga
    24241 gagcattaaa atgggtaaac tcacgggcaa gacagcactg attacgggcg cattgcaggg
    24301 aattggcgaa ggaattgcca gaacttttgc gcgtcatggc gcaaacctaa tcttgctgga
    24361 tatctcccct gagatcgaaa agctggcgga cgaactgtgt ggtcgtggtc atcgctgtac
    24421 ggcggttgtc gccgatgtgc gtgacccggc gtcggtagcc gcagctatca aacgcgcgaa
    24481 ggaaaaagaa gggcgcattg atatcctggt gaataacgca ggcgtttgtc gtctgggcag
    24541 tttcctcgat atgagcgatg acgatcgcga tttccatatt gacatcaata ttaaaggcgt
    24601 atggaacgtc acgaaggcgg tgctgccgga gatgattgcc cgcaaagatg gtcgcattgt
    24661 gatgatgtct tcggtcactg gcgatatggt ggccgatcct ggcgaaacgg cgtacgcctt
    24721 aacgaaagcg gcgattgttg gcctgacaaa atcgctggcg gtggagtacg cgcagtctgg
    24781 tattcgcgtt aacgccattt gcccgggata cgtgcgcaca ccaatggcgg aaagcattgc
    24841 ccgccagtcg aacccggaag atccagagtc ggtgctgact gaaatggcga aagcaatccc
    24901 gatgcgtcgc ctcgccgatc cgctggaagt cggcgaactg gcggccttcc tcgcatcgga
    24961 tgaatccagc tatttaaccg gtacacagaa tgtgattgat ggcggcagca cactgccgga
    25021 gacggttagc gtcggtatct gattcacctc tgtttcctcc ctgcatttgt ggggaggatt
    25081 tcgttttaaa cccggttctt attccctttt caacttccaa atcaccaaac ggtatataaa
    25141 accgttactc ctttcacgtc cgttataaat atgatggcta ttagaaagtc attaaattta
    25201 taagggtgcg caatggccgt taacttactg aaaaagaact cactcgcgct ggtcgcttct
    25261 ctgctgctgg cgggccatgt acaggcaacg gaactgctga acagttctta tgacgtctcc
    25321 cgcgagctgt ttgccgccct gaatccgccg tttgagcaac aatgggcaaa agataacggc
    25381 ggcgacaaac tgacgataaa acaatctcat gccgggtcat caaaacaggc gctggcgatt
    25441 ttacagggct taaaagccga cgttgtcact tataaccagg tgaccgacgt acaaatcctg
    25501 cacgataaag gcaagctgat cccggccgac tggcagtcgc gcctgccgaa taatagctcg
    25561 ccgttctact ccaccatggg cttcctggtg cgtaagggta acccgaagaa tatccacgat
    25621 tggaacgacc tggtgcgctc cgacgtgaag ctgattttcc cgaacccgaa aacgtcgggt
    25681 aacgcgcgtt atacctatct ggcggcatgg ggcgcagcgg ataaagctga cggtggcgat
    25741 aaagccaaaa ccgaacagtt tatgacccag ttcctgaaaa acgttgaagt gttcgatacc
    25801 ggcggtcgtg gcgcaaccac cacttttgcc gagcgtggcc tgggcgatgt gctgattagc
    25861 ttcgaatcgg aagtgaacaa catccgtaaa cagtatgaag cgcagggctt tgaagtggtg
    25921 attccgaaaa ccaacattct ggcggaattc ccggtggcgt gggttgataa aaacgtgcag
    25981 gccaacggta cggaaaaagc cgccaaagcc tatctgaact ggctctatag cccgcaggcg
    26041 caaaccatca tcaccgacta ttactaccgc gtgaataacc cggaggtgat ggacaaactg
    26101 aaagacaaat tcccgcagac cgagctgttc cgcgtggaag acaaatttgg ctcctggccg
    26161 gaagtgatga aaacccactt caccagcggc ggcgagttag gcaagctgtt agcggcgggg
    26221 cgtaactgat gtttgctgtc tcctccagac gcgtgctgcc gggctttacc ttaagcctcg
    26281 gcaccagtct gctgtttgtg tgcctgattt tgctgctgcc gctctccgcg ctggtgatgc
    26341 aactggccca gatgagctgg gcgcagtact gggaggtgat caccaacccg caggtggtcg
    26401 cggcctacaa agtaacgctg ctgtcggcgt ttgtggcatc gatttttaac ggcgttttcg
    26461 gtctgctgat ggcgtggatc ctaacccgct atcgcttccc aggccgcacg ctgcttgatg
    26521 cgctgatgga tttacccttt gcgctgccaa cggctgtcgc cggtttaacg ctggcctcgc
    26581 tcttttccgt aaacggtttt tacggtgaat ggctggcgaa gtttgatatc aaagtcacct
    26641 atacatggct ggggattgcg gtggcgatgg cctttaccag cattccgttt gtggtgcgta
    26701 ccgtgcagcc ggtgctggaa gagttaggcc cggaatatga agaagcggcg gaaacgcttg
    26761 gtgcaacgcg ctggcagagt ttctgcaaag tggtgctgcc ggagctttct ccggcgctgg
    26821 tggcgggcgt ggcgctgtcg tttacccgta gtcttggtga atttggcgcg gtgattttta
    26881 tcgccggaaa tatcgcgtgg aagacggaag tgacgtcgct gatgattttt gtgcgcttgc
    26941 aggagtttga ttacccggcg gcgagcgcga ttgcttcggt gatcctcgcg gcatccctga
    27001 tactgctgtt ctcaattaac actctgcaaa gtcgctttgg tcggcgtgtg gtaggtcatt
    27061 aatggcggaa gtgacacaat tgaagcgtta tgacgcgcgc ccgattaact ggggcaaatg
    27121 gtttctgatt ggcatcggga tgctggtatc ggcgttcatc ctgctggtgc cgatgattta
    27181 tatcttcgta caggcgttca gcaaagggct gatgcctgtt ttacagaatc tggccgatcc
    27241 ggatatgctg cacgccatct ggctgacggt gatgatcgcg ctgattgccg taccggtaaa
    27301 cctggtgttc ggtattctac tggcgtggct ggtgacgcgc tttaacttcc ccggacgcca
    27361 gttgctgctg acgctgctgg acattccgtt tgccgtgtcg ccggtggttg ccggtctggt
    27421 gtatttgctg ttctacggct ctaacggccc gctcggcggt tggctcgacg agcataacct
    27481 gcaaattatg ttctcctggc cgggaatggt gctggtcacc atcttcgtga cgtgtccgtt
    27541 tgtggtgcgc gaactggtgc cggtgatgtt aagccagggc agccaggaag acgaagcggc
    27601 gattttgctt ggcgcgtccg gctggcagat gttccgtcgc gtcacattac cgaacatccg
    27661 ctgggcgctg ctttatggcg tggtgttgtc caacgcccgc gcaattggcg agtttggcgc
    27721 ggtgtcggtg gtttccggct cgattcgcgg cgaaaccctg tcgctgccgt tacagattga
    27781 attgctggag caggactaca acaccgtcgg ctcctttacc gctgcggcgc tgttaacgct
    27841 gatggcgatt atcaccctgt ttttaaaaag tatgttgcag tggcgcctgg agaatcagga
    27901 aaaacgcgca cagcaggagg aacatcatga gcattgagat tgccaatatt aagaagtcgt
    27961 ttggtcgcac ccaggtgctg aacgatatct cactggatat tccttcaggt cagatggtcg
    28021 cgttgctggg gccgtccggt tccgggaaaa ccacgctgct gcgcattatc gccgggctgg
    28081 agcatcaaac cagcgggcat attcgcttcc acggcaccga cgtcagccgc ctgcacgcgc
    28141 gcgatcgcaa ggtcggtttc gtgttccagc attacgcgct gttccgccat atgacggtat
    28201 tcgacaatat cgcttttggc ctgacggtgc tgccgcgtcg cgatcgcccg aatgccgcag
    28261 ccatcaaagc gaaagtgaca aaattgctgg agatggtgca gcttgctcat ctggcagatc
    28321 gttatccggc acagctttcc ggcggccaga aacagcgcgt ggcgctggcg cgcgcgctgg
    28381 ctgtagaacc gcaaattctg ctgcttgatg aaccgtttgg cgcgctggat gcgcaggtgc
    28441 gtaaagagct gcgtcgctgg ctgcgtcaac tgcatgaaga gctgaaattc accagcgtgt
    28501 tcgtgaccca cgaccaggaa gaggcgaccg aagtagccga tcgtgtagtg gtgatgagtc
    28561 aggggaatat tgaacaggct gatgcgccga atcaggtatg gcgcgaacca gcgacccgtt
    28621 ttgtactcga atttatgggc gaagtgaacc gcctgcaggg aaccattcgc ggcgggcagt
    28681 tccatgttgg cgcgcatcgc tggccgctgg gctacacacc tgcgtatcag ggtccggtgg
    28741 atctcttcct gcgcccgtgg gaagtggata tcagccgccg taccagtctc gattcgccgc
    28801 tgccggtaca ggtactggaa gccagcccga aaggtcacta cacccaatta gtggtgcagc
    28861 cgctggggtg gtacaacgaa ccgctgacgg tcgtgatgca tggcgacgat gccccgcagc
    28921 gtggcgagcg tttattcgtt ggtctgcaac atgcgcggct gtataacggc gacgagcgta
    28981 tcgaaacccg agatgaggaa cttgctctcg cacaaagcgc ctgataggtt gagtgaatgt
    29041 taaacgcccg gaggcgcttc ccgcgatccg ggctttttaa tggcaaggtt tgtaacctgt
    29101 agacctgata agacgcgcaa gcgtcgcatc aggcaacacc acgtatggat agagatcgtg
    29161 agtacattag aacaaacaat aggcaatacg cctctggtga agttgcagcg aatggggccg
    29221 gataacggca gtgaagtgtg gttaaaactg gaaggcaata acccggcagg ttcggtgaaa
    29281 gatcgtgcgg cactttcgat gatcgtcgag gcggaaaagc gcggggaaat taaaccgggt
    29341 gatgtcttaa tcgaagccac cagtggtaac accggcattg cgctggcaat gattgccgcg
    29401 ctgaaaggct atcgcatgaa attgctgatg cccgacaaca tgagccagga acgccgtgcg
    29461 gcgatgcgtg cttatggtgc ggaactgatt cttgtcacca aagagcaggg catggaaggt
    29521 gcgcgcgatc tggcgctgga gatggcgaat cgtggcgaag gaaagctgct cgatcagttt
    29581 aataatcccg ataaccctta cgcccattac accactaccg ggccggaaat ctggcagcaa
    29641 accggcgggc gcattaccca ttttgtctcc agcatgggaa caaccggcac tatcaccggc
    29701 gtttcacgct ttatgcgcga acaatccaaa ccagtaacga ttgttggcct gcagccggaa
    29761 gaaggcagca gtattcccgg cattcgccgc tggcctgcgg aatatctgcc ggggattttc
    29821 aacgcttctc tggtggatga ggtgctggat attcatcagc gcgatgcaga aaacaccatg
    29881 cgcgaactgg cagtgcggga aggaatattc tgtggcgtca gctccggcgg cgcggttgcc
    29941 ggagcactgc gggtggcaaa agctaaccct ggtgcggtgg tggtagcgat catctgcgat
    30001 cgtggcgatc gctacctttc caccggggtg tttggggaag agcattttag ccagggggcg
    30061 gggatttaag attattgcgc aggcaccgtt ttatccgtct gcgcaattag cgcatgtaaa
    30121 aaaggctgtg cgttctcatc gctcttaccg gggatcttca caatatacgg cttcccggta
    30181 atcgatgacg acgaagcaac cttatcaata aactgctcgg cagtgtcaat gcggttacgg
    30241 gtattgccca gcttcagacg cagatgagaa accgcttcat cgcaggtatg ttcatcaccg
    30301 ttgcgcacaa atatcaaatc ctttttctgt gctaatccct ccagcatggc gttgatgcgg
    30361 gcttcttcat gggcagttaa cttcgcgtaa gcggggagtg tcattagtag tgtaatgacc
    30421 agacaaatga ttttcttcat tgaattacct tatccttgtt ttgtgatagc cgatttgact
    30481 gccagaatca ggtaaagttt cggttggaat acgatgagga tcatcggcaa ggagaaaaaa
    30541 ttaaatgagt agtttgttgt tgtttaacga taagagcagg gcgctgcagg cggatatcgt
    30601 cgccgtgcag tcgcaggtgg tttacggcag cgtgggcaac agcattgccg tgcctgctat
    30661 caaacagaac ggtctgaatg tttttgccgt gccgacggta ttgctgagca atacgccgca
    30721 ttatgacact ttctacggtg gtgcgattcc ggatgaatgg tttagcggtt atttgcgtgc
    30781 gcttcaggag cgtgatgcgc tgcgccagct gcgcgctgta accacgggct atatgggaac
    30841 ggcatcgcaa atcaaaatcc ttgccgagtg gctgactgcg ctacgcaaag accatcctga
    30901 cctattgatc atggtcgatc cggtgattgg cgatattgat agcggaattt atgtcaaacc
    30961 tgaccttccc gaggcgtatc gacaatattt actgccgctg gcgcagggaa ttacccccaa
    31021 tatctttgag ctggaaatcc tgaccggtaa aaattgccgc gatctcgaca gtgccattgc
    31081 tgccgcaaaa agtctgcttt cagacacatt aaaatgggtg gtgattacca gcgcctccgg
    31141 taatgaagaa aatcaggaga tgcaggtggt ggtggtcagt gccgacagcg tgaatgtcat
    31201 ttcccattca cgggtaaaaa ccgacctgaa agggactggc gacctgtttt gtgctcagct
    31261 tatcagtggt ttgctgaaag ggaaggcgtt aaacgatgcg gtgcaccgtg cggggttgcg
    31321 cgtactggaa gtgatgcgct acacccagca gcatgagagc gatgaattga ttttgccgcc
    31381 gctggcggaa gcataaaaaa tggcgccgat gggcgccatt tttcactgcg gcaagaatta
    31441 cttcttgatg cggataaccg gggtttcacc cacggttacg ctaccggaca gtttgatcag
    31501 ttctttgatt tcgtccatgt tggagataac aaccggagtc agggtagact tggctttctc
    31561 ttccagtagc ggcagatcaa attcaatgac agtatcgcca actttcacgc gctgaccttc
    31621 ttcagcaata cgcttgaagc cttcgccttt cagttcaacg gtgtcgatac cgaagtggac
    31681 gaacagttca acgccgctat cagattcgat agagaacgcg tgattggttt caaagatttt
    31741 accaatggta ccgtctacag gcgcgaccat tttgttaccc gttggtttga tagcaatacc
    31801 atcaccaacg attttttccg caaaaacgac atccggcacg tcttcgatat tgacgatctc
    31861 gccagagagc ggagcaatga tctcaatagt tccggtatcc ttcttgtcgt cggaaaccag
    31921 agatttcagt ttatcgaaca aacccatgat cttctcctaa gcagtaaatt gggccgcatc
    31981 tcgtggatta gcagattgtt ttttcttcaa tgaacttgtt aaccagcgtc atcaactcgt
    32041 ccgttgtcgg ttgagcaaga gcctgctctg ctaacacctt cgcatcttcg aagttcgtgt
    32101 tacggataat cttcttaatg cgcgggatag aaatggcgct catagagaat tcgtccagac
    32161 ccatccccag caacagaagt gtagcacgtt catcgccagc aagctcacca cacatgccag
    32221 tccatttgcc ttcagcatga gaagcatcaa taacttgctt gatcaagttc agcacggacg
    32281 gtgacattgg ttggtaaagg tgtgaaatca tatcattacc acggtcaact gccagagtgt
    32341 actgcgttaa atcattggtg ccgatactaa agaaatcaac ttctttggct aaatgacgtg
    32401 caattgttgc ggcagccggt gtttccacca ttacgccgat ttcaattgac tcgtcaaacg
    32461 ctttaccttc gtcgcgcagt tcctgtttgt agatttcgat ctctttgcgc agtgcacgca
    32521 cttcttcaac agagatgatc atcgggaaca taatgcgcaa tttaccgaaa gccgaggcac
    32581 gcaggatagc gcggagctga tcgcgcagga tctctttacg atccatcgcg atacggatag
    32641 cgcgccagcc gaggaacggg ttctcttctt tcgggaagtt catgtatggc agctctttgt
    32701 cgccgccgat gtccatggta cgaacgataa ccgcttgcga gccacacgct tcagccactg
    32761 ctttgtaagc agcaaactgt tcttcttcag tgggcagcgc gtcgcggtcc atgaacagga
    32821 actcagtacg atacagacca acgccttcag cgccgttacg ctctgcacct tcaacgtcac
    32881 gaaccgtacc aatgttagcg catacttcta cctggtgacc gtccagcgta atagctggca
    32941 gatctttcag tttagcaagc tctgcttttt cagaagccac ttgctcctga acagcgcgca
    33001 ttttatcaat aacttcgttg gttggattga cgtaaacctg attatttacg gcatccagaa
    33061 tcagatagtc gtcatttttc acctgagagg tgacgctacc ggtacccacg atagcaggca
    33121 gttccagaga acgcgccatg atagaggtgt gggaagtacg gccacccgcg tcggtgatga
    33181 aacccagcac cttcttcagg ttcagctgtg cggtttcgga cggcgtcagg tcagcggcaa
    33241 ccagaatgac ttcatcctga atggcgctca ggtcgataat cttcaggccc aggatgttgc
    33301 gcagcaggcg cttaccgata tcacgtacgt cagccgcacg ttctttcagg tattcatcat
    33361 ccagctcttc cagggcagaa gcctgacctt cgataacttc atgagcagct gcgtcagctg
    33421 tcatgtgctt atctttaatc agggctatga tttcctgctc cagctcctca tcttcgagca
    33481 gcataatatg cccttcaaag atggcttctt tttcttcacc gaacgtttca ccagctttcg
    33541 ttttgatcgt ttccagctgg gctgatgcct tggcacgacc gctcagaaaa cgttcaactt
    33601 cctgatcaac ctggtcggca gaaatttttt tccggtcaat gacaatttcg tcttctttca
    33661 gaagcagagc tttaccgaaa gcgatacccg gggatgctaa aatgcctgaa atcataaccc
    33721 taccttactt gtgactgatt tttaaaagaa cccgggaaat tactcgagtt ccgccatcag
    33781 tttaaccaga tgttcaaccg ctttctgctc gtcttcgcct tctgcggaga tagtcacaac
    33841 ggtaccttga gtcaggccca gagtctgcag tttaaacagg cttttcgcgc tggcgctttt
    33901 gccgttggaa gtcacagtaa tttcagaagt gaagccctta gcttctttta caaactgggc
    33961 agcagggcgg gtgtgcagac cgttcggagc ggtaatggta acttcttgct ggaacattgt
    34021 atttccccaa cttataggtt tagtgttgtg gaactaaagt ctagcctggc ggttcgactt
    34081 tagcctgtat tgttagcgcc agtttttaac agacgcgacg cacgaagagc gcaatacagt
    34141 ccattgctgg cgggtgttga ccgctgacgt ttcgtacgtc attaaacatt atgccgctaa
    34201 aggaagaatt gaaccaaatc ataaaatcga ttcagctttg gcttttcctg taacgattaa
    34261 tttcgcgcat caaaataatt agtctggtta aataccagtt ccgacaggct gaatcaatgc
    34321 caggaggggc gaaagtttga agcaggccac aaaaaagcac ctaaaaaggt gcttttttac
    34381 gcatttttaa caagctggca ttactgttgc agttctttct cagtgaagag atcggcaaac
    34441 aatgcggtgc ttaaataacg ctcacccgat gacggtagaa taaccacaat attcttgttg
    34501 gtaaagcttt catcttcttg tagtttcaac gccgcagcaa cagctgctcc agaagagata
    34561 cctgcaagaa taccttcttc ttccatcaga cgacgtgcgg tagaaatcgc ttcttcattg
    34621 gtgatgccaa tgactttatc gaccagcttg agatcgaggt tagctgggat aaaaccagcg
    34681 ccaataccct gaattttatg cgggccaggt ttaatctctt caccagccag tgcctgggcg
    34741 ataactggag aatcggttgg ctcaacggcg acagagataa gatcggtctt gcctttggtg
    34801 cctttaatgt agcggctgac gccagtcagc gtaccgccag tcccaacgcc agcaataaat
    34861 acatcaacct gaccgtcggt atcttcccag atctccggac cggtggtttt ttcgtgaatt
    34921 tcagggtttg ccggattgct gaattgttgc agcagcaggt atttctctgg attgctggcg
    34981 acaatttctt ctgctttttg gattgcgcct ttcatgcctt tagcaccttc cgtcagcacc
    35041 aggtttgcac ctaacgcttt cagcagcttg cggcgttcaa tactcatggt ttctggcatg
    35101 gtcagggtga gtttgtaacc gcgagcggca gctacatagg ccagtgcaat cccggtatta
    35161 ccgctggtcg gttcaaccag ttcaacgcct ggtttcagca cgccgcgctt ttcggcatcc
    35221 caaatcatgt tggcaccgat acggcactta acgctgaagc tggggttacg agattccacc
    35281 ttcgccagaa tgcgtccgtt accgatgcga ttcaggcgaa ccagcggcgt gtgaccgata
    35341 gtcagcgagt tatcttcaaa aatcttactc atggcctgtc cttaactgta tgaaattggg
    35401 atacaacagg tagcataccc gctcagagaa tatgcggaag taaggattta gcatatctat
    35461 atacagaagg gaaataatga catgcaagat ggaataaggg gcggcataag ccacccctgt
    35521 ttcacacaaa cggtttacaa aataaccggt agattgttac cgccacatcg cgtgtttatc
    35581 gcgatagcaa tcgacccaca tcgccgtcgc gccacaaacg gcaacgggca tgatgaacag
    35641 attaagcagc gggatcatcg taaacaggct ggttaaagca ccaaactgca tattggtgat
    35701 tttgcgtgtg cgcagggcgg tgcgcatctc tttaaacggc actttgtggt tatcgaaggg
    35761 gtaatcgcaa tactggatgg ctaacatcca ggcgctaaac aggaaccaca gtaccggcgc
    35821 gacggtttgc ccaataccag ggattaagta aagaattagc aatacaattg cgcgcggcag
    35881 ataccaggca aatttttgcc attcgcgttt catgattcgc ggcacatctt tcatgatacc
    35941 gaaaatcccg gtatctggcg gtgtagcgcc agtcaatcgt gcttccagtt gttcagccaa
    36001 taaaccgtta aacggagcgg caatccagtt agcaatcgtg gagaagaaat agccaaacac
    36061 taacagcaca gagatgacag ccagaggcca caacagataa ctcagccatt gtagccagtc
    36121 cggaacgtaa ctcatgagag tcgggatcca gacatcgagc tgtgtaaaga gccaccagaa
    36181 tgcgcccccc atcaacaaaa tattgaccag cagcggtaaa ataacgaaac gccgaatccc
    36241 aggttgcgag acgagcttcc agccttgcgc aaaatagtaa aaaccgctgc gtggggcaga
    36301 tgtgaatgat gaaaccataa tcaggatgag ctccttttga ccaatcccag gaaaattctg
    36361 cgtattttac cgggtaattg cgcaatggac agttaggata tgttcgaaaa aacagcaaaa
    36421 agcacgattt catctatctt tgtgctgtga aagttaatag tgcacttgca cttgaggtaa
    36481 tcggcaaata ctcttagtga gtaaatgttt gccgtggtgg caaggtgtta gaacaacaga
    36541 gaatataatg atgcaggatt tgcgtctgat attaatcatt gttggcgcga tcgccataat
    36601 cgctttactg gtacatggtt tctggaccag ccgtaaagaa cgatcttcta tgttccgcga
    36661 tcggccatta aaacgaatga agtcaaaacg tgacgacgat tcttatgacg aggatgtcga
    36721 agatgatgag ggcgttggtg aggttcgtgt tcaccgcgtg aatcatgccc cggctaacgc
    36781 tcaggagcat gaggctgctc gtccgtcgcc gcaacaccag taccaaccgc cttatgcgtc
    36841 tgcgcagccg cgtcaaccgg tccagcagcc gcctgaagcg caggtaccgc cgcaacatgc
    36901 tccgcgtcca gcgcagccgg tgcagcagcc tgcctatcag ccgcagcctg aacagccgtt
    36961 gcagcagcca gtttcgccac aggtcgcgcc agcgccgcag cctgtgcatt cagcaccgca
    37021 accggcacaa caggctttcc agcctgcaga acccgtagcg gcaccacagc ctgagcctgt
    37081 agcggaaccg gctccagtta tggataaacc gaagcgcaaa gaagcggtga ttatcatgaa
    37141 cgtcgcggcg catcacggta gcgagctaaa cggtgaactg cttcttaaca gcattcaaca
    37201 agcgggcttc atttttggcg atatgaatat ttaccatcgt catcttagcc cggatggcag
    37261 cggcccggcg ttattcagcc tggcgaatat ggtgaaaccg ggaacctttg atcctgaaat
    37321 gaaggatttc actactccgg gtgtcaccat ctttatgcag gtaccgtctt acggtgacga
    37381 gctgcagaac ttcaagctga tgctgcaatc tgcgcagcat attgccgatg aagtgggcgg
    37441 tgtcgtgctt gacgatcagc gccgtatgat gactccgcag aaattgcgcg agtaccagga
    37501 catcatccgc gaagtcaaag acgccaacgc ctgatacact taaggcaaat taactcctct
    37561 tcgaaccccc gcttgtcggg ggtttttagc attgatggtg cgatatggaa tcaatcgaac
    37621 aacaactgac agaactgcga acgacgcttc gccatcatga atatctttat catgtgatgg
    37681 atgcgccgga aattcccgac gctgaatacg acaggctgat gcgcgaactg cgcgagctgg
    37741 aaaccaaaca tccagaactg attacgcctg attcgcctac ccaacgtgta ggcgctgcgc
    37801 cgctggcggc tttcagccag atacgccatg aagtaccaat gctgtcactg gataacgttt
    37861 ttgatgaaga aagctttctt gctttcaaca aacgtgtgca ggaccgtctg aaaaacaacg
    37921 agaaagtcac ctggtgctgt gagctgaagc tggatggcct tgccgtcagt attctgtatg
    37981 aaaatggcgt tttagtcagt gccgcgaccc gtggcgatgg caccaccggg gaagatatca
    38041 cgtctaatgt gcgtactatt cgcgctattc cgctgaagct gcacggagag aatatcccgg
    38101 cgcgtctgga agtgcgtggt gaagtgttcc tgccgcaggc ggggttcgaa aagattaacg
    38161 aagatgcgcg acgcacgggc gggaaagttt ttgctaaccc acgtaatgcg gcagctggtt
    38221 cactgcgtca gcttgatccg cgtattacag cgaagcgacc gctcactttt ttctgctatg
    38281 gcgttggtgt tctggaaggt ggcgagctgc cggatactca tcttggccgt ttactgcaat
    38341 ttaaaaagtg ggggttgccg gtcagcgatc gggtaacgct ttgtgaatcg gcggaagaag
    38401 tgctggcgtt ctatcacaaa gtggaagaag accgcccgac gctgggcttt gatatcgacg
    38461 gcgtggtgat taaggtcaac tcactggaac agcaggagca gcttggcttt gtcgcgcgtg
    38521 ccccgcgctg ggcggtagcg tttaaattcc cggcgcagga gcagatgacc tttgtgcgtg
    38581 acgtcgagtt tcaggttggg cgtactggcg cgattacgcc tgttgcgcgt ctggaacctg
    38641 tccatgttgc tggcgtgctg gtgagtaacg caaccttaca caatgcggat gaaatcgaac
    38701 gtcttggttt acgcattggc gataaagtgg tgattcgccg cgctggcgac gtgatcccgc
    38761 aggtggttaa cgtcgtgctt tctgaacgcc cggaagatac ccgtgaggtt gtattcccga
    38821 cgcattgtcc ggtatgtggt tctgacgttg agcgtgtgga aggtgaagcg gttgcccgct
    38881 gtaccggtgg cctgatttgc ggagcgcagc gtaaagagtc gctgaaacac tttgtttccc
    38941 gccgtgcgat ggatgttgac ggaatgggcg acaaaatcat cgatcagctg gttgaaaaag
    39001 aatatgtcca tactccggca gatctgttca aactcaccgc aggcaaactg accggactgg
    39061 agcgtatggg gccaaaatcg gcacaaaacg tggttaacgc gctggaaaaa gcgaaagaaa
    39121 ccacctttgc tcgcttcctc tatgcacttg gcatccgtga agtcggcgag gccaccgcag
    39181 caggtctggc ggcatatttc ggcacgctgg aagcgctgga agccgcttcg attgaagagc
    39241 tgcaaaaggt gcctgatgtt ggcattgtcg ttgcatccca cgttcacaac ttctttgccg
    39301 aagaaagcaa ccgcaatgtc atcagcgagc tgttggcgga aggtgttcac tggcctgcgc
    39361 cgatcgttat caacgcggaa gagattgaca gcccgtttgc tggtaaaacc gtggtgctta
    39421 cgggcagctt aagccagatg tcgcgtgatg acgctaaagc tcgactggtt gaactgggcg
    39481 cgaaagtcgc gggcagcgtg tcgaagaaaa ccgatctggt gattgccgga gaagcggcag
    39541 gttctaaact ggcgaaagcg caggaactgg gcattgaagt catcgacgaa gcggaaatgc
    39601 tgcgtttgct gggtagctga gatggaaaaa gagcagctga ttgaaatagc caatacgata
    39661 atgccgtttg gcaaatataa agggcgtcgc ttaatcgacc tgccggagga gtatctgctg
    39721 tggtttgccc gcaaagatga attcccggca gggaagctcg gtgagctaat gcaaatcacg
    39781 ctgctgatta aaaccgaggg gctgacgcaa ctggtccagc ccctgaagcg tccgctttaa
    39841 gctttatcgg cgctgctttc ctgctgtgcc tgtaactgtt cggtctggcg tttgtatcga
    39901 cgcgccagca ccgcacagac catcaattgg atctgatgga aaatcatcag gggcagcacc
    39961 atcataccga tcaccgatgt ggggaacaga atgtttgcca tcgggatgcc atttgccaga
    40021 ctctttttcg aaccacaaaa gacgatagta atttcatctg ccttattgaa gcccagtcgg
    40081 cgtgccataa agacgttaac tacaatcacg atagccagaa gaacgcagct gaccaccacg
    40141 ataaacagca atgatcccca gccaacttta tgccagatac cattaacgac ggcttcgctg
    40201 aacgctgtat aaaccaccaa cagaatggac gtctggtcag ttttcgcaat ccatttttta
    40261 ttgcgcgaca cccagtcacc aatccacggc cgggaaagat gccccaacac aaacggcagc
    40321 agcagttgca gcataatttt accgacctgc tcaaggctgc cccctgcacc gtgaacattc
    40381 atcaccagac caaccagcaa tggtgaaagg aaaatcccca gcaggctgga tgctgacgca
    40441 gaacaaaccg ccgccgcgac gttaccgccc gccattgacg tgaaggcgat tgcagactgc
    40501 acggtagccg ggagaatgca caagtagaga aaaccggagt agagcatcgg gtcgacattt
    40561 accggtttcc accaggcaaa cagtacaccc agaatcggaa acagcacgaa ggtgctgcaa
    40621 attacccaca aatgcagtcg ccagtgacca ccgccagcaa taatcgcctc acgcgacaac
    40681 ttcgcgccgt gcataaagaa cagcagggca atcgctgcgg tggtcagatt ttcaaagaag
    40741 gggacgaaat cgcctctggc cggaaagaaa gaggccagca acaccaccgt gatcagggtt
    40801 aaggtgaaag gatcgaggat acgaaaaagt ttcataaaga ctccagatga tcgatggttc
    40861 tattgtgctt cttccagttt gagaaataaa attgatttat tgcatccatt catgaagaaa
    40921 acagatgaat tattctttaa aacaattaaa ggttttcgtc acagtagcgc aggagaaaag
    40981 ttttagtcgt gcaggagagc gtatcggcct gagccagtcg gcagtgagtc acagtgtgaa
    41041 ggaactggaa aatcatactg gtgttcgcct gctggacaga accacgcgtg aagtggtgct
    41101 tacagatgca gggcagcagc tggctttgcg tcttgagcga ctgctggatg aactgaacag
    41161 cacgttgcgc gataccgggc gtatggggca acaactgagc ggaaaagttc gggtcgctgc
    41221 cagccagacc atttccgcgc atcttattcc gcaatgcatt gctgaaagcc atcgccgcta
    41281 tccagatatt cagtttgtcc tgcacgatcg tccgcagcag tgggtgatgg aaagtattcg
    41341 ccagggagat gtcgactttg gcatcgtcat cgatccaggc ccagtgggcg atctgcaatg
    41401 tgaagcgatt ctttccgagc ctttctttct gctttgccat cgcgatagcg ctttggccgt
    41461 cgaagattat gtaccctggc aggcattaca gggagcgaaa ttggtgttgc aggattacgc
    41521 gtcaggcagc cgaccgctga ttgacgcagc gctggcgcgc aatggtattc aggcgaatat
    41581 tgtgcaggag attggacatc cggcgacgct gttcccgatg gtagccgcag gcatcggcat
    41641 cagtattctt cccgcactgg cgctaccatt gcctgaaggt agcccactgg tggttaagcg
    41701 cattacgccg gtggttgaac accagttgat gctggtgcgc aggaagaacc gttcactttc
    41761 caccgccgct gaagcgttat gggacgtggt acgagaccag ggcaatgcgc taatggctgg
    41821 gcgggaaggg gatccgttgt atcagatata gatatcgata tgagtcgttt tttcaacagg
    41881 atttagaagg gatacattca gagacaaaca ctgctctttc tcctgcgcct tttctgcctg
    41941 ctgtttctgc aactgcgcca gttgtgtttc cagtagggtg atttgctgtt gaatcaattc
    42001 ggcctgtttt tgcttgtctt cagcactgcc gctagtgtct acaatctctt taatttgttg
    42061 agtgagtttg atgatttgct gacttatccg cgcgatttgt gaagcaacat cgctaccttg
    42121 tgatgccctg ttactactct gtgtttgtat ggaaggaatt gaagttgaag agatagtggc
    42181 cattgttcag tcctcgtcac tgtacgaggt aatatcggta gaagaacaag caacttgaga
    42241 agaaaaatca atcttacaaa tcgcagatag caaaaaagcg cctttagggc gcttttttta
    42301 cattggtggg tcgtgcagga ttcgaacctg cgaccaattg attaaaagtc aactgctcta
    42361 ccaactgagc taacgacccg aagtggtggg tgatgacggg atcgaaccgc cgaccccctc
    42421 cttgtaaggg aggtgctctc ccagctgagc taatcacccc cgctgtgtgg agtcgcatta
    42481 tagggagagt tcaaaatgag tcaacgcatt ttctaaagaa attgttcgtt cgtcgtaaat
    42541 ttaagcaaga tgatcgcaaa acagaccgtg ttgcgcaatt tgtcaacgaa aacaataatg
    42601 cgtaaggttg caacccgaac tacattgagg aatcaggcgg gagtgataga atatcgccca
    42661 ctcaattttt ccaggatttg ccggttgtcg gcatctttct aaacgtaagg ccatttcatg
    42721 aaaatcaaaa ctcgcttcgc gccaagccca acaggctatc tgcacgttgg cggcgcgcgt
    42781 actgctcttt actcctggct ttttgcacgt aaccacggcg gtgagttcgt gctgcgtatt
    42841 gaagacaccg atcttgagcg ttccacgccg gaagctatcg aagccattat ggatggcatg
    42901 aactggctga gcctggagtg ggatgaaggt ccgtactacc agaccaaacg ttttgatcgc
    42961 tacaatgcgg tgatcgatca gatgctggaa gagggcactg cttataaatg ctattgctct
    43021 aaagagcgcc tggaagcgct gcgcgaagag caaatggcga aaggtgagaa gccgcgttat
    43081 gacggtcgct gccgccacag ccatgagcat catgctgatg atgaaccgtg tgtcgtgcgt
    43141 tttgctaatc cgcaggaagg ttctgttgtt tttgacgatc agatccgtgg tccgatcgag
    43201 ttcagcaacc aggagctgga tgatctgatt atccgccgta ccgatggttc cccaacctat
    43261 aacttctgtg tggttgtcga tgactgggat atggaaatca cccacgttat ccgtggcgaa
    43321 gaccatatca acaacacgcc gcgccagatc aacattctta aggccctgaa agcgccggtg
    43381 ccggtttacg cgcacgtttc tatgatcaat ggcgatgacg gtaaaaaact gtccaaacgt
    43441 cacggggcag tcagcgtaat gcagtatcgt gatgacggtt atttgccaga agcactgctg
    43501 aactatctgg tgcgtctggg ctggtcccac ggcgatcagg aaatcttcac tcgtgaagag
    43561 atgatcaaat acttcacttt gaatgccgtc agcaaatctg ccagtgcgtt caacaccgac
    43621 aagctgctgt ggctgaacca tcactacatt aacgcgctgc cgccggagta tgttgctact
    43681 cacttacagt ggcacattga gcaggaaaat atcgataccc gtaacggccc gcagctggct
    43741 gatctggtga aactgctggg cgaacgctgc aagacgctga aagagatggc acagagctgc
    43801 cgttatttct acgaagattt tgctgagttc gatgccgatg cggcgaaaaa acatctgcgt
    43861 ccggtagcgc gtcagccgct ggaagtggtt cgtgacaaac tggccgcgat tactgactgg
    43921 accgctgaaa acgttcatca cgctattcag gcgacggcgg atgagctgga agtgggtatg
    43981 ggtaaagttg gtatgccgct gcgtgtcgcc gtaaccggtg cggggcagtc tccagcactg
    44041 gatgttaccg ttcacgcaat tggtaagacc cgcagtatcg agcgtatcaa caaagcgctg
    44101 gattttattg ctgaacgcga aaatcagcag taattagcgt gtaaaaagat aaacggcagg
    44161 agataatatc ctgccgtttt ttatttatgc tgattcgtca atatcgagac gggcgaggaa
    44221 attgcgaatt ccttcacgtg acaaaaattg cgcgactttt tcctgctcaa tgtctgtcat
    44281 attatctatc gcgttgatga tttgtcgata cgccggggcg cgtgctccag gagcgtattc
    44341 agcaattctc tcttcagctt ccatcaacat tggtgtgtta tggaaggctg gcgtgttctg
    44401 aataaactcg caaacttgcg tatcgaccag aataagtctg gcacgaccgc ctttcacgcc
    44461 gggaaatttt tccgttttcc agcctttttc tctgatccaa cggttaacag tttgtttagc
    44521 cacaccgagg cattcagcca gttcttcggt ggtcattttg ttgcgtaatc ttttcatatt
    44581 atttgctatc gcgaatccct aagcgttgta acaatccgat aatcccttcc cgcagaagca
    44641 aggatgtaaa ctgtttttgt tcaactggcg tcatctcttt cgccagtgtc accagcaata
    44701 cttcaagcgg cgcatcacca gaaagggcag gcgcttctcc ctgaccttct ggacgttcag
    44761 cattgcgaat atattcacga acctgttcat tgacgtgaac cagtcgggct ttgccaccct
    44821 ggacgccagg ttttggtgac gttgtccagc cttccttgcg tacccattta ttaatggtct
    44881 ggcggctata gccggtcagt ctggcaagtt catctggtgt catcctctcc ttgaacataa
    44941 caattccctg aatgtatgtg ggtctattag tagttcattt tataacacca ctttacagga
    45001 aaccgtgaca tgaataacta ctgaatgcga gcggttttcc aaaattgttc atttgcctga
    45061 tttttcagcg attgaaatta cacgttgcaa tttgccgttg acacattcgg gcggaattca
    45121 tatgatgccg cccgtcaact cgacaagctt tacgtgacgg ggctatagct cagctgggag
    45181 agcgcttgca tggcatgcaa gaggtcagcg gttcgatccc gcttagctcc accaaatttt
    45241 gcacccagca aatttggtac gtaaacgcat cgtggggcta tagctcagct gggagagcgc
    45301 ttgcatggca tgcaagaggt cagcggttcg atcccgctta gctccaccaa atttccaacc
    45361 ctcgctgcaa agcgggggtt ttttgtctct gctttttgcc gcttttgtaa tacagtctac
    45421 gtccgggtta gcgccgcctg gtgaaagcat cattggatga aaaatcggca acaggctggc
    45481 ccctgtttgc ttcgcgatgc gaataaactt attatttgtg tgcctgaaaa ccccgatcag
    45541 tgagagtagt gtactcatgt ttgtggagca taacctgata aaaaatatca agatattcac
    45601 actagcgttt acgctcaccg tggtacttat tcagctatcc cgttttattt cgccacttgc
    45661 cattatccat tccagttata tctttctggc gtggatgcca ctgtgcgtaa tgctgtcaat
    45721 cttgtttatc tttggctggc gcggtgtcgt tcccgtttta tgcgggatgt tttgcaccaa
    45781 tctgtggaac tttcatctct cttttttaca gactgcggtc atgctcggta gccagacgtt
    45841 tgtcgtgttg tgtgcctgcg caatattacg ctggcagctg gggacgcgtt ggcgttatgg
    45901 attgaccagc cgatatgtct ggcaacgtct gttctggctt ggtttggtga cgccgatcgg
    45961 catcaaatgc agcatgtatc ttgtggggag cttctttgat ttcccgctaa agatatccac
    46021 ctttttcggc gatgcggatg ccattttcac ggtcgttgat ttgctaagcc ttttcaccgc
    46081 tgtgctgatt tacaacatgc ttttctacta tctcactcgc atgattgtaa gtccccactt
    46141 tgcgcagata ttgtggcgca gggatatcgc gccgtcgttg ggcaaagaga aacgcgcatt
    46201 taccttaagc tggctggcag cgttaagcgt gcttctactt ctgttgtgca caccttatga
    46261 aaacgacttt attgccggtt acctggtacc cgttttcttc atcatcttta ccctcggggt
    46321 cggtaagctt cgctatccgt ttttaaatct cacctgggct gtttcaacgc tttgccttct
    46381 gaattacaac cagaactttt tgcaaggggt ggaaaccgaa tattcgctgg catttattct
    46441 tgcggtgctg atttccttta gcgtttgcct gctctatatg gtgcgcattt atcatcgcag
    46501 tgaatggctt aatcgccgct ggcatttgca ggtgctgaca gatccgttaa cgctcctacc
    46561 caactttcgt gcgttggaac aagcgccgga acaagaggcg ggcaagagtt tttgctgcct
    46621 gcgcattgat aatcttgagt ttatgagtcg tcattacggc ttaatgatgc gcgttcactg
    46681 tatccgctca atttgccgta cgctgctgcc gttgatgcag gaaaacgaaa agttgtatca
    46741 attgccgggt agtgaactgc tgttagtgct gagcgggccg gaaacggaag ggcgactcca
    46801 gcatatgatt aacatcctga atagtcggca aattcactgg aacaataccg ggctggatat
    46861 gggctatggt gctgcctggg ggcgttttga tggaaatcag gaaaccctgc aacccttgtt
    46921 ggggcagtta agctggctgg cggagcaatc ctgcgcacat catcatgtgc tggcgctgga
    46981 tagcagagag gagatggttt ccgggcagac cactaaacag gtgctattgc tgaataccat
    47041 tcgcacggcg ttagatcagg gtgatttgct gctctacgcc cagccaattc gcaacaaaga
    47101 gggtgaaggt tatgatgaga tcctcgcgcg actgaaatat gacggcggca ttatgacccc
    47161 ggataagttt ctgcccctta ttgctcagtt taaccttagc gcgcgttttg atttgcaagt
    47221 gctggaatcc ttgttgaagt ggctggcaac acacccttgc gacaaaaaag gaccgcgctt
    47281 ttcagtcaat ttaatgccgc tcacgctgct gcaaaagaat attgccgggc ggattattcg
    47341 tctgtttaag cgttatcaca tctccccgca ggcggtcatt cttgagatca ccgaggagca
    47401 ggcgttttct aacgcagaaa gcagcatgta caacatcgag cagctgcata agtttggttt
    47461 ccggattgcg attgatgact ttggcaccgg atatgccaac tacgaacggt taaagcgttt
    47521 gcaggctgat atcatcaaaa ttgatggcgt ctttgtgaaa gatattgtca cgaacacgct
    47581 ggatgcgatg attgtgagat caattaccga tctggcgaaa gcgaagtcat tgagtgtggt
    47641 cgcggagttt gtcgagacgc aacagcagca ggcgctattg cataagctcg gggtgcaata
    47701 tctgcaaggg tatttgattg gtcgcccgca gccattagct gattaacagg cgtaaaaaaa
    47761 ccggggaatt atccccggct tttttatgta tggtcttaca gcaccagtgc tgcgattgac
    47821 gcagacagca cactcaccag ggtagagccg taaaccagct tcagaccgaa gcgagaaacc
    47881 acgttacctt gctcttcatt caggccttta actgcacctg cgataatccc gattgaagag
    47941 aagttagcga aggaaaccag gaacacagag atgatgcctt cagcacgcgg agagagcgtg
    48001 gaagcaattt tctgcagatc catcatcgca acgaactcgt tggaaaccag tttggtcgcc
    48061 atgatactgc ccacttgcag tgcttcactg gaaggaacac ccatcaccca tgcaatcgga
    48121 tagaagatgt agcccaggat gccctggaag gagatgctgt agccaaacca gccagtaacg
    48181 gtggcaaaca gtgcgttcag cgcggcgatc agggcgataa agccgatcag catcgcggca
    48241 acgataatgg caactttgaa acctgccaga atgtattcac ccagcatttc gaagaagctc
    48301 tgaccttcgt gcaggttgga catctggatg ttttcttcac tggcatcaac acggtaagga
    48361 ttgatcagcg acagcacgat aaaggtgctg aacatgttca gtaccagcgc agcaacgacg
    48421 tatttcggtt ccagcatggt catgtatgca ccaacgatgg acatcgacac ggtggacatt
    48481 gccgtggcag ccatggtgta catacgatta cgggagattt tgccgaggat atctttatag
    48541 gcaataaagt tttcagactg acccagaatc agggagctga cggcgttaaa ggattccagt
    48601 ttgcccatgc cgttgacttt ggagagcagg aaaccaattg cgcggatgat caccggcaac
    48661 acgcgaatgt gctggagaat accgatcagt gcagagataa agacgattgg gcacagcact
    48721 ttcaggaaga agaatgccag gccttgatca ttcatgctac caaagacgaa gttagtccct
    48781 tcgttggcaa atccgagcag tttttcgaac atttcggaga agcctttcac gaagcctaaa
    48841 ccaacgtcgg agttcaggaa gaaccacgcc agtaacactt cgataacaag cagttgaata
    48901 acataacgga tacgaatttt tttgcggtcg ctgcttacca gcagtgcgag aatcgcaaca
    48961 acggcaagtg ccagtacaaa atgaaggacg cggtccatat ttgctccaaa tatgaggcag
    49021 gttaaatttc cgtgcacatt ctatgtaaca tggttaagga aaacgagatc tgacacacac
    49081 tataatgacg tcctgtgaca agcttcaaaa atagtgatct gtcatacatt tctgctatgt
    49141 tgtgtatgga agctgaaagt tatgtaaatg tgctaacatt aaacataacc ataacattca
    49201 agtcatcaat cttttcgtag tcataatcct ggtctatcaa agaaatcacc acaacccatt
    49261 caaatgaatt tgataatcat tctcgtttgg catagcatga aacatagcaa aggctatgtt
    49321 ttagaggcac aagatgacga actatcgcgt tgagagtagc agcggacggg cggcgcgcaa
    49381 gatgaggctc gcattaatgg gacctgcgtt cattgcggcg attggttata tcgatcccgg
    49441 taactttgcg accaatattc aggcgggtgc cagcttcggc tatcagctac tgtgggttgt
    49501 cgtttgggcc aacctgatgg cgatgctgat tcagatcctc tctgccaaac tagggattgc
    49561 caccggtaaa aatctggcgg agcagattcg tgatcactat ccgcgtcccg tagtgtggtt
    49621 ctattgggtt caggcagaaa ttattgcgat ggcaaccgac ctggcggaat ttattggtgc
    49681 ggcgatcggt tttaaactca ttcttggtgt ttcgttgttg cagggcgcgg tgctgacggg
    49741 gatcgcgact ttcctgattt taatgctgca acgtcgcggg caaaaaccgc tggagaaagt
    49801 gattggcggg ttactgttgt ttgttgccgc ggcttacatt gtcgagttga ttttctccca
    49861 gcctaacctg gcgcagctgg gtaaaggaat ggtgatcccg agtttaccta cttcggaggc
    49921 ggtcttcctg gcagcaggcg tgttaggggc gacgattatg ccgcatgtga tttatttgca
    49981 ctcttcgctc actcagcatt tacatggcgg ttcgcgtcaa caacgttatt ccgccaccaa
    50041 atgggatgtg gctatcgcca tgacgattgc cggttttgtc aatctggcga tgatggctac
    50101 agctgcggcg gcgttccact tttccggtca tactggtgtt gccgatcttg atgaggcata
    50161 tctgacgctg caaccgttgt taagccatgc tgcggcaacg gtctttggat taagcctggt
    50221 tgctgccgga ctgtcctcaa cggtggtggg gacactggcg gggcaggtgg tgatgcaggg
    50281 gttcattcgc ttccatatcc cgctgtgggt gcgtcgtaca gtcaccatgt tgccgtcatt
    50341 tattgtcatt ctgatgggat tagatccgac acggattctg gttatgagtc aggtgctgtt
    50401 aagttttggt atcgccctgg cgctggttcc actgctgatt ttcaccagtg acagcaagtt
    50461 gatgggcgat ctggtgaaca gcaaacgcgt aaaacagaca ggctgggtga ttgtagtgct
    50521 ggtagtggcg ctgaatatct ggttgctggt aggaacggcg ctgggattgt agttgaatga
    50581 gcgtcgcatc tggcactatt ggcggatgcg gcgtaaacgc cttatccgcc ctacgcgtta
    50641 aagagccggt ttgtaggcct gataagacgc gctagcgtcg catcaggcat tgtgctccat
    50701 tggcgaatgt gtttaatggc ggtgtccatg tccgcggcct tttccgcgat gatcgtcacg
    50761 atcgcgccag ccttcacgat agccacgctc atacgcttta cgcttatccc agccacgatg
    50821 atagccatta tcatgacgcc accagcggtt tttacgccat tcataattgc gatgccagta
    50881 gtcacggtcg cgccagtgac caccgtccca gtaattaccg taatgatcgc gatcgccaat
    50941 ttgtaatttg attgatggca gtagggtgat ttcacctgcg tttgctacaa gcggggtaaa
    51001 tgccatcagg gcggccgcca gaaacagtga cctgaacatt gttattctcc ttcacgatcg
    51061 aagccgtcag cggcctgtta acgcaatatt acggggaggt aaagccccgt atcatcgcca
    51121 taactcttaa atcataaagg agagcatttt ttgctaaacg ttatcgtggg gtggctgttg
    51181 cttcggacga ttgaccatca tcatcggctt acctgctaat aacagccatg ccggaagcat
    51241 gacgatacag agcagcggta gcaatgtggt attgggtaca acgactgccg ccataaaaag
    51301 acttaaccag ccatcgcgtg ttaccaccag cacgatgccg agaatagcgc aagaaacggt
    51361 tattgccgct ggtacggcgg gaacgtgctc atgcagcatc aaccctaatg ccacgccgac
    51421 aaacactgcc gggaagattc gcccaccgcg aaagccactg gcggcagcaa cgaccagggc
    51481 ggcaagttta attaccgcca gcaaaaagta atcgctggtg ctgaaagcct gatttgccac
    51541 catctgctgc atctcatcca gccctttaaa cagcgaaact ggtccaccaa taacccccag
    51601 aataccgaga ataaatccgc caatacccag cacgagcacc ggatttttca tttgatgcat
    51661 cattgagtgc aaccgtggta agcaccatac ggcgaccatc cctgctgcga tggcaatcgc
    51721 cgcgacaatt gcaccgctga gaatatcggt catttccatc tgtccgtaat gcgcaatggg
    51781 cagtgaaaaa tgaggatgga aaaataatcc ggtagtaagt gcaccagctg ctgccgccat
    51841 taacggcgca aagagacgat cccatagcgg aacttcacta ctgccattta aggtttgcga
    51901 aaatatcaac gccgccgcaa caggtgtgcc aaacagcgcg ccgatggttc cggcagaggc
    51961 taaaatagtc cactccattc ggttgacgcg cggtaacaga cgagcgccaa tggcaaccgc
    52021 aagggcgata ttgacggtca tgatcggatg ttctggcccc aggctgacgc caccagcaag
    52081 accaagaatt aatgctacga taagtcctgg tagcgcagag ggcggaaccg gtgcgccgat
    52141 cagcggttca caggcggggt ctggtccggc atgaccctgg ctgaaacgga taaccaaccc
    52201 caccgcaata cccgttagcg ttaatacacc gatgatccag aggggtgaat cctgggctat
    52261 ccccagagtt cccggcagtc gttgccagag caaattctgt aatgccgagg cgattttcat
    52321 caccacaatc agaataagac tggacgcaat cccaattgcc acggcgggga gcgataataa
    52381 caacatggtt ctggctcgcg gatggagcat gttctattcc ttatgcgggg tcagatactt
    52441 agtttgccca gcttgcaaaa aggcatcgct gcaattggtg ctgaaacgat aaagtaaatg
    52501 tgtgacccag atcgatattt acagggagcc tgcctttccg gcgttgttgt tatgccccca
    52561 ggtatttaca gtgtgagaaa gaattatttt gactttagcg gagcagttga agaatgacaa
    52621 agtatgcatt agtcggtgat gtgggcggca ccaacgcacg tcttgctctg tgtgatattg
    52681 ccagtggtga aatctcgcag gctaagacct attcagggct tgattacccc agcctcgaag
    52741 cggtcattcg cgtttatctt gaagaacata aggtcgaggt gaaagacggc tgtattgcca
    52801 tcgcttgccc aattaccggt gactgggtgg caatgaccaa ccatacctgg gcgttctcaa
    52861 ttgccgaaat gaaaaagaat ctcggtttta gccatctgga aattattaac gattttaccg
    52921 ctgtatcgat ggcgatcccg atgctgaaaa aagagcatct gattcagttt ggtggcgcag
    52981 aaccggtcga aggtaaacct attgcggttt acggtgccgg aacagggctt ggggttgcgc
    53041 atctggtcca tgtcgataag cgttgggtaa gcttgccggg cgaaggcggt cacgttgatt
    53101 ttgcgccgaa tagtgaagaa gaggccatta tcctcgaaat attgcgtgcg gaaattggtc
    53161 atgtttcggc ggaacgcgtg ctttctggtc ctgggctggt gaatttgtat cgcgcaattg
    53221 ttaaagccga caaccgcctg ccagaaaatc tcaagccaaa agatattacc gaacgcgcgc
    53281 tggctgacag ctgcaccgat tgccgccgtg cgttgtcgct gttttgcgtc attatgggcc
    53341 gttttggcgg caatctggca ttaacgctgg gaacctttgg tggggtttat attgctggcg
    53401 gtatcgtgcc gcgcttcctt gagttcttca aagcctctgg tttccgtgcc gcatttgaag
    53461 ataaaggacg ctttaaagaa tatgtccatg atattccggt gtatctcatc gtccataaca
    53521 atccgggtct tctcggttcc ggtgcacatt tacgccagac cttaggtcac attctgtaaa
    53581 tccttcctgt tatatcggga ggtaactctc ccgataatct tttaaatcat acagttaatt
    53641 caatttttct ttgtgtcccc tcacaaggcc gacctgcgtc acacttccgt acagcgggat
    53701 taattctcca gtaaatgcat tatttgtctg gtaacggcga tttgttttgc acgttcataa
    53761 tttcactcgt caactatcac tgaatgagga attaacgatg agtaagaaac tgattgcctt
    53821 atgtgcctgc ccaatgggcc tggctcacac ctttatggcc gctcaggcgt tggaagaagc
    53881 ggcggtagaa gccggttatg aagtgaaaat tgaaacccag ggcgcggacg gtatccagaa
    53941 tcgcctgacg gcgcaggata tcgccgaagc aaccatcatc atccactccg tggcagttac
    54001 cccggaagat aatgagcgtt tcgaatcacg cgacgtttat gaaatcactt tgcaggacgc
    54061 aattaaaaac gctgcgggca tcatcaaaga aatcgaagag atgattgcct ctgaacaaca
    54121 gtaatattcg acagggataa ttgttatggc cattaaaaaa cgcagtgcaa ccgttgtgcc
    54181 tggcgcatcc ggtgcggcag cggcagttaa gaatccgcag gcctctaaaa gcagcttctg
    54241 gggtgaactc ccgcagcatg tgatgtcagg gatttcacgc atggtgccaa ccttaattat
    54301 gggcggtgtg atcctcgctt tcagccagct gattgcttat agctggctta aaattcctgc
    54361 tgagatcggc atcatggatg ctcttaatag cgggaaattc tccggtttcg acctctcctt
    54421 acttaaattt gcctggctgt cgcagtcctt cggcggcgtg ttgtttggtt ttgccattcc
    54481 gatgtttgct gcttttgtgg cgaactctat cggtggcaaa ctggcgttcc cggctggttt
    54541 tatcggcggg ttgatgtcta cccagccgac gcaactgctg aacttcgatc ccagcactat
    54601 gcaatgggcg acctcttcgc cggtgccgtc aaccttcatt ggtgcgctga ttatttctat
    54661 cgtcgcgggt taccttgtga agtggatgaa ccagaaaatc cagttaccgg atttcctgtt
    54721 agcgttcaaa accacatttt tgctaccgat tctttccgcc atttttgtca tgctggcgat
    54781 gtactacgtc atcaccccct ttggtggctg gatcaacggc ggtatccgta ctgtgctgac
    54841 tgccgcaggt gagaagggcg cgctgatgta cgcaatgggg atcgtcgcgg cgacagcaat
    54901 cgaccttggt ggcccgatca acaaagccgc aggtttcgtt gccttcagct ttaccactga
    54961 ccacgtatta ccggtcaccg ctcgttctat cgctatcgtt attcctccga ttggtctggg
    55021 tctggcgacc attattgacc gtcgtttaac cggcaaacgc ctgttcaacg ctcaacttta
    55081 tccgcagggt aagaccgcga tgttccttgc ctttatgggg attagtgagg gtgcgattcc
    55141 gtttgcgctg gaaagcccca tcaccgccat tccgtcgtat atggtcggcg cgattgtcgg
    55201 ctcaaccgcc gctgtctggc tgggcgcagt gcaatggttc ccggaatccg ctatctgggc
    55261 atggccgctg gtcactaacc tgggcgtcta tatggcgggg atcgcgctgg gggcagtcat
    55321 aactgcgctg atggtggtgt tcctgcgtct gatgatgttc cgtaaaggca aattgttaat
    55381 cgatagcctg taagaaggaa aatccagatg acattactcg cttcgctgcg cgactggctt
    55441 aaggcgcaac aactggatgc agtgcttctc tcctcacggc agaacaaaca gccgcatctg
    55501 gggatctcca ccggatcagg ctatgtggtt attagccgtg aaagtgcgca cattctggta
    55561 gattcgcgct atttcgttga ggtggaagcc cgtgcgcaag gctaccagct gcatttgctt
    55621 gacgcgacga acacgcttac cactatcgtc aatcaaatca ttgccgatga gcagttgcaa
    55681 acgctcggtt ttgaaggcca gcaggtgagt tgggaaaccg cgcaccgctg gaagtctgaa
    55741 ctcaatgcga aactggttag cgccacgccg gatgtgctgc ggcaaatcaa aacgccagag
    55801 gaggtggaga taatccgcct cgcctgtggg attgccgatc gcggagcgga gcatattcgc
    55861 cgctttattc aggcggggat gagcgagcgc gagatagccg ctgaactgga gtggtttatg
    55921 cgccagcagg gcgcagaaaa agcctctttt gacaccattg tcgccagcgg ctggcgcggg
    55981 gcgctgccgc acggcaaagc cagcgacaag attgttgcag cgggcgagtt tgtcactctc
    56041 gattttggtg cgctgtatca gggctactgc tctgatatga cgcgcacctt gctggtgaat
    56101 ggcgaagggg tgagcgccga atctcacccg ctgtttaacg tctaccagat tgtcctgcag
    56161 gcacagctcg cagcaatctc tgcaattcgc cccggcgtgc gctgccagca ggttgacgat
    56221 gccgcgcgcc aagtgattac cgaggcaggt tttagccact atttcggtca taacaccggt
    56281 catgctatcg gcattgaagt tcatgaagat ccgcgttttt caccgcggga caccacgacg
    56341 ctacagccag gcatgttact gaccgtggag ccggggattt atttgccagg gcaagggggc
    56401 gtgcgcatcg aagatgttgt gctggttacc ccgcaaggcg cagaagtgct ctacgccatg
    56461 ccgaaaacag tgttgctcac gggagaggca taatggattt atcgctatta aaagcgttga
    56521 gcgaggcaga tgcgatcgcc tcctcggaac aggaagtgcg gcagatcctg ctggaagaag
    56581 cggatcgcct gcaaaaagaa gtgcgatttg atggtctggg atcggtgctg atccgcctca
    56641 atgaatcgac aggtccgaag gtgatgatct gcgcccatat ggacgaagtg ggatttatgg
    56701 tgcgtagcat ctcccgcgaa ggggcaattg atgtgctgcc ggttggcaac gtgcgcatgg
    56761 ctgcccgcca gctgcagccg gtgcgcatca ccacccgtga agagtgcaaa attccaggcc
    56821 tgcttgacgg cgaccggcag gggaatgacg tcagcgccat gcgcgtggac attggtgcgc
    56881 gctcgtatga cgaagtgatg caggcgggaa ttcgtccagg cgatcgcgtc acctttgata
    56941 caacttttca ggttctccct caccagcggg tgatggggaa agcctttgat gatcgcctcg
    57001 gttgctatct gctggtgacg ttactacgtg agctgcacga tgccgaactg cctgcggagg
    57061 tgtggctggt ggcaagttcc agcgaagagg tgggattacg cggtgggcaa accgctaccc
    57121 gcgcggtgtc gccggacgtc gccattgtgc tggataccgc ctgctgggcg aaaaactttg
    57181 attatggcgc ggctaaccat cgccagattg gtaacggccc gatgctggtg ttaagcgaca
    57241 agtcactgat tgcgccgcca aaacttaccg cctggatcga aaccgtggcg gctgaaattg
    57301 gcgtaccgtt gcaggcagat atgttcagca acggcggcac ggacggcgga gcggtgcact
    57361 taaccggtac tggcgtaccc acagtggtaa tggggcctgc cacccgccac ggacattgcg
    57421 cggcatcgat tgccgattgc cgcgacattt tgcagatgga gcaactttta tctgccctta
    57481 ttcaacgtct tacgcgtgag acggttgttc aactgacgga tttcagatga tctcctgatt
    57541 aacccggagc ggttatgtta acgattcaat ttctctgtcc tttgcctaac ggtctacatg
    57601 ctcgtccggc gtgggaactt aaagaacagt gcagccagtg gcaaagcgaa atcacctttt
    57661 tcaaccatcg ccagaacgca aaggccgatg ctaaaagctc gctggcgttg attggaactg
    57721 gcaccttatt taatgacagt tgcagcctga acattagcgg cagcgatgaa gagcaggcgc
    57781 ggcgcgtgct ggaagagtac atcctggtgc gctttatcga cagcgacagc gttcagccga
    57841 cgcaggcaga actgacggcg catccgctgc cgcgttcatt aagccgcctt aacccggatt
    57901 tactgtacgg caatgtgctg gcaagcggcg tcggcgtggg tacgctgacc ctgttacaaa
    57961 gcgacagcct cgacagttat cgggcaatcc ccgccagtgc gcaagattcc acccggctgg
    58021 agcacagcct ggcaacgctt gccgagcaac tgaaccagca attgcgtgag cgtgacggcg
    58081 aaagcaaaac tatcctcagc gcccatttgt cgctgattca ggatgatgaa ttcgcgggca
    58141 atatccgtcg tctgatgaca gaacagcatc aagggctggg ggcggcgatc atcagcaata
    58201 tggagcaggt ttgcgccaaa ctttctgctt ctgccagtga ttatctgcgc gaacgtgtta
    58261 gcgacattcg cgatattagc gaacagttgc tgcatatcac ctggccggaa ctgaagccgc
    58321 gcaacaatct ggtgctggaa aaaccgacta ttctggtagc cgaggattta accccaagcc
    58381 agtttttgag cctcgattta aaaaatcttg caggcatgat tctggagaaa actgggcgca
    58441 cctcgcatac cctgattctg gcccgcgcct cggcgatccc ggtactgagc ggcctgccgc
    58501 tggatgcgat tgcccgttat gccgggcaac cggcggtgct tgacgcccag tgcggcgtgc
    58561 tggcgattaa cccgaatgac gcggtgagcg gttattatca ggtcgcacag acgctggcgg
    58621 ataaacgcca aaaacaacag gcgcaggccg ccgcgcaact ggcctattcc cgtgataaca
    58681 agcgtattga tattgcggcg aatatcggca ccgctctgga agcgccaggc gcgtttgcca
    58741 acggcgcgga aggcgttgga ctgttccgta ctgaaatgct gtatatggat cgtgacagcg
    58801 cgccggacga gcaggagcaa tttgaagcct accagcaggt actactggca gcgggcgaca
    58861 agccgattat cttccgcaca atggacatcg gtggcgataa aagcattcct tatctgaata
    58921 ttccccagga agagaacccg ttcctcggct atcgcgcggt acgtatttac ccggaatttg
    58981 ctggcctgtt ccgcactcaa ctacgggcga ttttgcgcgc cgccagtttt ggcaacgccc
    59041 agttgatgat cccgatggtc cacagtctcg atcagatctt atgggtgaag ggcgagatcc
    59101 aaaaagcgat cgttgagctt aagcgcgatg gcctgcgtca tgcagagacg attacgctgg
    59161 ggatcatggt ggaagtgccg tcggtgtgct acatcatcga tcacttctgc gatgaggtcg
    59221 atttcttcag tatcggctcc aacgatatga cccagtatct gtatgcggtc gatcgtaata
    59281 acccgcgcgt atcgccgctg tataacccga ttacgccatc gttcctgcgc atgttgcagc
    59341 agattgttac cactgcgcat cagcggggca aatgggtagg tatttgcggt gaactgggcg
    59401 gtgaaagccg ttatctgccg ctactgcttg ggctgggcct ggatgagctg agtatgagta
    59461 gcccgcgtat tccggcggtg aaaagccagc ttcgtcaact ggatagcgag gcgtgtcggg
    59521 aactggcgcg tcaggcatgt gaatgccgca gtgcgcagga aattgaagcg ttactcaccg
    59581 cctttacgcc ggaagaagac gttcgcccac tgctggcgct ggagaatatc tttgttgatc
    59641 aggattttag caataaagag caggcgatcc agttcctgtg cggcaacctc ggcgttaacg
    59701 ggcgcactga acatccgttt gagctggaag aagatgtctg gcagcgggaa gagattgtca
    59761 ctaccggcgt tggttttggc gtggcgatcc cgcacaccaa atctcagtgg atccgtcatt
    59821 ccagtatcag cattgcccgg ctggtgaaac cggttgactg gcagtcagag atgggcgaag
    59881 tcgaactggt gatcatgctg acgttgggtg ctaacgaagg gatgaaccat gtgaaagtct
    59941 tctcgcagct ggcgcgtaaa ctggtgaata aaaacttccg ccagtcattg tttgccgcgc
    60001 aagatgcgca aagcatcctg acgctgctgg aaacggaatt aaccttctga cgttagccct
    60061 gaaaacgggc gctgtactct cccggcgtca gaccaaactg acgccggaaa acgcgacaaa
    60121 aatagtcgct gtccggaaaa ccgcaacgct gtgccacttc atgaattgac agatgatatt
    60181 tctgcaaaat catcctcgcc ttcgccattc gcacccaacg cacatactcg ataaaacgca
    60241 tcgttccatg ctgagcaaac agttttgaca gatgattggg cgtgatatta aaaaactgcg
    60301 ccacgctctc gcgggtgagc ggctgggcat aattatcctg aacccagtta caaatgctgt
    60361 gatagagaaa atcgccgcgc ggttgcgaat tacccggcgg catattcacc acgttgcggc
    60421 aaagatgcag caaacttaac accagtggct gaatgatatt ctgctccagc ggcgaacggc
    60481 taagatgggt gagggcggta agcatcgctt caccttcacc gcgttgcagg ctgggaagct
    60541 ggatctggcg agcaggatgc aacaacgacg tggtgcggct atcgtaaaac gataacccaa
    60601 gccaggtcgg tgcgaacacc aggcttaaca gcatcaccgg tttgttgttg atcggtaaat
    60661 tagccgcgcg tgccggaata aacagcattt ctccttccac cattcgctgc tgctttgctt
    60721 ccagcaggtt gccgtattcg ccgcgcagca cgatatccag acggggaaaa tcaatacata
    60781 aactgcccac cggtaacgag gcaggctggc tggcaaacca gacgcgcccc agtagttgcg
    60841 ggttaagcac caggccgctg aacagatcgg cgaaaaattg ttgatcggcg ggcaagcctg
    60901 gtgctttcat gaaagttccc gattaaagat gcattaactg gcgaaattct ttcactttgc
    60961 tgcggctgac cggcacttca aaatccagat ctttcagtcg cagaatgtag gtgttattaa
    61021 accacggttc gatttcgcgt attttgttca gattgacaca aaatgagcga tggcagcgga
    61081 aaaaatgcga cggcggcagt ttgctgcaaa attcggtaat gttcatcggc attacgtagg
    61141 attcacgccg cgtatagaca aaggtcattt tctcgtgcgc ttcggcgtaa tagatatcgt
    61201 taattggcgt gacgattatt cgctcatctt tcaccagatt aatggtgtca ttctcacgcg
    61261 ttaccgtcgc ggcaggcgta ctgctggtct gctgttgttg ccaggccgct tccagttttt
    61321 gcagcatccc ggtaatacgt gactcctgat acggtttgag aatgtagtca aacgcctcca
    61381 gttcaaacgc ttctaccgca tgttctttcc acgcggtgat gaacacaata aacggtttat
    61441 gggcgaactg gctgatgttt tgcgccagca acacgccatc cagcgacgga atattgatat
    61501 ccagaaaaat ggcgtcgacg cggttatgct gcaaaaactt caacacgtcc agaccgtcgt
    61561 caaaggtgcc gacaatctcc atctggctgt gctctttaat tagccagctc agttcctgtt
    61621 gtgccaggaa ttcgtcttca acaatgatga ctttcacaat atcactccgg ctcaaagcaa
    61681 taacgtagcc tgtgaggcga ctggggtgcg ttggttagga atgtaaaacg caatttccgt
    61741 ccccggctcc aggcggcgga tatgcagccc ctcgccatac aataacttca cgcgatgatg
    61801 gacattcagc aggccaattt tattgcccgg catttcattc gcttcgaccc gctcaatcac
    61861 ctttggatcg atgccgtgcc cggtatctcg caccgcaatg cgtacccgat ttccgcactc
    61921 tgcaacgctg atggtgacaa cgcctttacc tttgcaaggc tgaataccgt ggacaatggc
    61981 gttctccacc aacggctgga tcagcaggct gggaatgcag caattcacct cttcatcaat
    62041 atcatagata accgtcagct tgtcaccaaa gcgggcctgc tcaatggcaa tatagtcttt
    62101 aatttgatac agctcttttt tgatatcgat ttgctcatcg tcttttaatt caatgttata
    62161 gcgcagataa cgcgacagat taaagatcaa ctggcgagcg gtatccggat tcagacggat
    62221 tgacgatgaa atagcgttca gagcgttaaa cagaaaatgg ggattaattt tgctttgcag
    62281 ggcgcgaagc tctgccttat ttgccatttc acgtagctgc tcggcgcgtg aaacctccag
    62341 ttgcgtggag atgatttgcg acagaccgac cgccatttcc tgtaatgacg aggtgatctg
    62401 atgcgcgtgg cagtagtaaa ttttcagcgt tccggtcacg acccctttct cccacaatgg
    62461 gatcaccagc atggaatgaa tctctggtgt gcggtgggct tcatcattgt ttttaatgat
    62521 gatttttccg taattcatcg cctgacgggt agtcgggcta atgaagtcat cgccattctg
    62581 atagttatgt tcacccacgc caacataggc cagcacatga tcggtattgg taattgccac
    62641 cgcatcggcg tgaatgtcat cgcggataat ttcgcagacc ttgcgtaatg actcgctatt
    62701 gacatggcga aacagcggta gcgttttgtt ggcgatatcc agcgccagct tggcctgccg
    62761 cgcggcgctg gcctcttttt cgccctcaac gctttgcacc agaagcacaa taaagccgat
    62821 acagacgcta ccgaggatca ttggaatgcc gattttagag acgatatcga tccccaacgc
    62881 ggtagttggt gcccagacaa ttaccagaat catggtcaac gtctcacaca acatgccgcc
    62941 gagaataccg acgcgccagc gctgtgcttt ggggattttc aggttgatcc agcccgatat
    63001 acaacccgcc agaatgctgg tgataaagca ggggatcgcc gtcacgccgc cgatatcaat
    63061 taaataccgg tgaatacccg caatcacgcc ggtaatgatg cctacccacg ggccaaacag
    63121 aatcccgccg gacatcaccg cgataatacg tacgttcacc agcgagcctt ctacgggaac
    63181 gccggaccag gtgctgaaca gggcgaacag cgagaaaatg gcggtaacgg cgagcaattc
    63241 ttttggggag tgcgccgact tgtgcaacag ttcgcgaaac aggcggatac ggatgagaaa
    63301 gaacaggcag ataagcatta acgctgcccg atcgaagacc gccagcagca tgttgaatat
    63361 ttcgtgcacg ggtgactctg aataaggatt aaacctctat gataggtaac ctgaaggctg
    63421 atgaccagca ggccgttttt gaggaaaaac cataaattta aaaggggatt gaaagataaa
    63481 tggtgtatta gca
//
LOCUS       DBANUU010000007        61815 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000007 DBANUU010000000
VERSION     DBANUU010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.007"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..801
                     /gene="ynaI"
                     /locus_tag="ACIE68_000419"
     CDS             <1..801
                     /gene="ynaI"
                     /locus_tag="ACIE68_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFX5906791.1"
                     /translation="IDFICTSLIAVILTIKLFLLINQFEKQQIKKGRDITSARIMSRI
                     IKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPF
                     SIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRI
                     TTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTK
                     TTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR"
     gene            complement(852..2465)
                     /gene="mppA"
                     /locus_tag="ACIE68_000420"
     CDS             complement(852..2465)
                     /gene="mppA"
                     /locus_tag="ACIE68_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFX5906792.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2802..3701)
                     /gene="pgrR"
                     /locus_tag="ACIE68_000421"
     CDS             complement(2802..3701)
                     /gene="pgrR"
                     /locus_tag="ACIE68_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFX5906793.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            3839..4759
                     /gene="ycjY"
                     /locus_tag="ACIE68_000422"
     CDS             3839..4759
                     /gene="ycjY"
                     /locus_tag="ACIE68_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFX5906794.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGV
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            4830..4967
                     /locus_tag="ACIE68_000423"
                     /pseudo
     CDS             4830..4967
                     /locus_tag="ACIE68_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
     gene            5095..5823
                     /gene="mpaA"
                     /locus_tag="ACIE68_000424"
     CDS             5095..5823
                     /gene="mpaA"
                     /locus_tag="ACIE68_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFX5906795.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCANPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(5798..6763)
                     /gene="ycjG"
                     /locus_tag="ACIE68_000425"
     CDS             complement(5798..6763)
                     /gene="ycjG"
                     /locus_tag="ACIE68_000425"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFX5906796.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADIDGPTWLAVDVEPALQFTTGELHL"
     gene            6882..7388
                     /gene="tpx"
                     /locus_tag="ACIE68_000426"
     CDS             6882..7388
                     /gene="tpx"
                     /locus_tag="ACIE68_000426"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFX5906797.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(7432..8973)
                     /gene="tyrR"
                     /locus_tag="ACIE68_000427"
     CDS             complement(7432..8973)
                     /gene="tyrR"
                     /locus_tag="ACIE68_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFX5906798.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(9121..10182)
                     /gene="ycjF"
                     /locus_tag="ACIE68_000428"
     CDS             complement(9121..10182)
                     /gene="ycjF"
                     /locus_tag="ACIE68_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFX5906799.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(10179..11576)
                     /gene="ycjX"
                     /locus_tag="ACIE68_000429"
     CDS             complement(10179..11576)
                     /gene="ycjX"
                     /locus_tag="ACIE68_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFX5906800.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            11731..12729
                     /gene="ycjW"
                     /locus_tag="ACIE68_000430"
     CDS             11731..12729
                     /gene="ycjW"
                     /locus_tag="ACIE68_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFX5906801.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(12840..13745)
                     /gene="ompG"
                     /locus_tag="ACIE68_000431"
     CDS             complement(12840..13745)
                     /gene="ompG"
                     /locus_tag="ACIE68_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFX5906802.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(13790..14872)
                     /locus_tag="ACIE68_000432"
     CDS             complement(13790..14872)
                     /locus_tag="ACIE68_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFX5906803.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIALR"
     gene            complement(14886..15545)
                     /gene="pgmB"
                     /locus_tag="ACIE68_000433"
     CDS             complement(14886..15545)
                     /gene="pgmB"
                     /locus_tag="ACIE68_000433"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFX5906804.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQN
                     V"
     gene            complement(15542..17809)
                     /gene="ycjT"
                     /locus_tag="ACIE68_000434"
     CDS             complement(15542..17809)
                     /gene="ycjT"
                     /locus_tag="ACIE68_000434"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFX5906805.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(17806..18861)
                     /gene="ycjS"
                     /locus_tag="ACIE68_000435"
     CDS             complement(17806..18861)
                     /gene="ycjS"
                     /locus_tag="ACIE68_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFX5906806.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLRDQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(18871..19659)
                     /gene="ycjR"
                     /locus_tag="ACIE68_000436"
     CDS             complement(18871..19659)
                     /gene="ycjR"
                     /locus_tag="ACIE68_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFX5906807.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(19677..20729)
                     /gene="ycjQ"
                     /locus_tag="ACIE68_000437"
     CDS             complement(19677..20729)
                     /gene="ycjQ"
                     /locus_tag="ACIE68_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFX5906808.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(20760..21602)
                     /gene="ycjP"
                     /locus_tag="ACIE68_000438"
     CDS             complement(20760..21602)
                     /gene="ycjP"
                     /locus_tag="ACIE68_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFX5906809.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(21589..22470)
                     /gene="ycjO"
                     /locus_tag="ACIE68_000439"
     CDS             complement(21589..22470)
                     /gene="ycjO"
                     /locus_tag="ACIE68_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFX5906810.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(22491..23783)
                     /gene="ycjN"
                     /locus_tag="ACIE68_000440"
     CDS             complement(22491..23783)
                     /gene="ycjN"
                     /locus_tag="ACIE68_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFX5906811.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEIMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELVEQH"
     gene            complement(23797..25476)
                     /gene="ycjM"
                     /locus_tag="ACIE68_000441"
                     /pseudo
     CDS             complement(23797..25476)
                     /gene="ycjM"
                     /locus_tag="ACIE68_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(25689..26003)
                     /gene="pspE"
                     /locus_tag="ACIE68_000442"
     CDS             complement(25689..26003)
                     /gene="pspE"
                     /locus_tag="ACIE68_000442"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFX5906812.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(26078..26299)
                     /gene="pspD"
                     /locus_tag="ACIE68_000443"
     CDS             complement(26078..26299)
                     /gene="pspD"
                     /locus_tag="ACIE68_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFX5906813.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(26308..26667)
                     /gene="pspC"
                     /locus_tag="ACIE68_000444"
     CDS             complement(26308..26667)
                     /gene="pspC"
                     /locus_tag="ACIE68_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFX5906814.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(26667..26891)
                     /gene="pspB"
                     /locus_tag="ACIE68_000445"
     CDS             complement(26667..26891)
                     /gene="pspB"
                     /locus_tag="ACIE68_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFX5906815.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(26945..27613)
                     /gene="pspA"
                     /locus_tag="ACIE68_000446"
     CDS             complement(26945..27613)
                     /gene="pspA"
                     /locus_tag="ACIE68_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFX5906816.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKDREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            27780..28757
                     /gene="pspF"
                     /locus_tag="ACIE68_000447"
     CDS             27780..28757
                     /gene="pspF"
                     /locus_tag="ACIE68_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFX5906817.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(28888..30153)
                     /gene="puuE"
                     /locus_tag="ACIE68_000448"
                     /pseudo
     CDS             complement(28888..30153)
                     /gene="puuE"
                     /locus_tag="ACIE68_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(30191..31471)
                     /gene="puuB"
                     /locus_tag="ACIE68_000449"
     CDS             complement(30191..31471)
                     /gene="puuB"
                     /locus_tag="ACIE68_000449"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFX5906818.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTATGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKMLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(31473..32960)
                     /gene="puuC"
                     /locus_tag="ACIE68_000450"
     CDS             complement(31473..32960)
                     /gene="puuC"
                     /locus_tag="ACIE68_000450"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFX5906819.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(33110..33667)
                     /gene="puuR"
                     /locus_tag="ACIE68_000451"
     CDS             complement(33110..33667)
                     /gene="puuR"
                     /locus_tag="ACIE68_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFX5906820.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(33694..34458)
                     /gene="puuD"
                     /locus_tag="ACIE68_000452"
     CDS             complement(33694..34458)
                     /gene="puuD"
                     /locus_tag="ACIE68_000452"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFX5906821.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            34670..36088
                     /gene="puuA"
                     /locus_tag="ACIE68_000453"
     CDS             34670..36088
                     /gene="puuA"
                     /locus_tag="ACIE68_000453"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFX5906822.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            36237..36401
                     /locus_tag="ACIE68_000454"
     CDS             36237..36401
                     /locus_tag="ACIE68_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5906823.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            36391..37776
                     /gene="puuP"
                     /locus_tag="ACIE68_000455"
     CDS             36391..37776
                     /gene="puuP"
                     /locus_tag="ACIE68_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFX5906824.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            37910..38155
                     /gene="ymjA"
                     /locus_tag="ACIE68_000456"
     CDS             37910..38155
                     /gene="ymjA"
                     /locus_tag="ACIE68_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFX5906825.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            38468..40111
                     /gene="sapA"
                     /locus_tag="ACIE68_000457"
     CDS             38468..40111
                     /gene="sapA"
                     /locus_tag="ACIE68_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFX5906826.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYDPAKSREQLKSLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            40108..41073
                     /gene="sapB"
                     /locus_tag="ACIE68_000458"
     CDS             40108..41073
                     /gene="sapB"
                     /locus_tag="ACIE68_000458"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFX5906827.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            41060..41950
                     /gene="sapC"
                     /locus_tag="ACIE68_000459"
     CDS             41060..41950
                     /gene="sapC"
                     /locus_tag="ACIE68_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFX5906828.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            41950..42942
                     /gene="sapD"
                     /locus_tag="ACIE68_000460"
     CDS             41950..42942
                     /gene="sapD"
                     /locus_tag="ACIE68_000460"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFX5906829.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            42944..43750
                     /gene="sapF"
                     /locus_tag="ACIE68_000461"
     CDS             42944..43750
                     /gene="sapF"
                     /locus_tag="ACIE68_000461"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFX5906830.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            43818..44171
                     /gene="ycjD"
                     /locus_tag="ACIE68_000462"
     CDS             43818..44171
                     /gene="ycjD"
                     /locus_tag="ACIE68_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFX5906831.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            44539..45327
                     /gene="fabI"
                     /locus_tag="ACIE68_000463"
     CDS             44539..45327
                     /gene="fabI"
                     /locus_tag="ACIE68_000463"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFX5906832.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            45471..46598
                     /gene="yciW"
                     /locus_tag="ACIE68_000464"
     CDS             45471..46598
                     /gene="yciW"
                     /locus_tag="ACIE68_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFX5906833.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            46666..48600
                     /gene="rnb"
                     /locus_tag="ACIE68_000465"
     CDS             46666..48600
                     /gene="rnb"
                     /locus_tag="ACIE68_000465"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFX5906834.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(48678..48782)
                     /locus_tag="ACIE68_000466"
     CDS             complement(48678..48782)
                     /locus_tag="ACIE68_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5906835.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            48836..50821
                     /gene="pdeR"
                     /locus_tag="ACIE68_000467"
     CDS             48836..50821
                     /gene="pdeR"
                     /locus_tag="ACIE68_000467"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFX5906836.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(50803..50931)
                     /gene="ymiD"
                     /locus_tag="ACIE68_000468"
     CDS             complement(50803..50931)
                     /gene="ymiD"
                     /locus_tag="ACIE68_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFX5906837.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAEYIYSSTYARFR"
     gene            50968..51141
                     /gene="yciZ"
                     /locus_tag="ACIE68_000469"
     CDS             50968..51141
                     /gene="yciZ"
                     /locus_tag="ACIE68_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFX5906838.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            51231..51980
                     /gene="yciT"
                     /locus_tag="ACIE68_000470"
     CDS             51231..51980
                     /gene="yciT"
                     /locus_tag="ACIE68_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFX5906839.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            52249..52467
                     /gene="osmB"
                     /locus_tag="ACIE68_000471"
     CDS             52249..52467
                     /gene="osmB"
                     /locus_tag="ACIE68_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFX5906840.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(52593..52919)
                     /gene="yciH"
                     /locus_tag="ACIE68_000472"
     CDS             complement(52593..52919)
                     /gene="yciH"
                     /locus_tag="ACIE68_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFX5906841.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(52919..53656)
                     /gene="pyrF"
                     /locus_tag="ACIE68_000473"
     CDS             complement(52919..53656)
                     /gene="pyrF"
                     /locus_tag="ACIE68_000473"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFX5906842.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(53849..55018)
                     /gene="lapB"
                     /locus_tag="ACIE68_000474"
     CDS             complement(53849..55018)
                     /gene="lapB"
                     /locus_tag="ACIE68_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFX5906843.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(55025..55333)
                     /gene="lapA"
                     /locus_tag="ACIE68_000475"
     CDS             complement(55025..55333)
                     /gene="lapA"
                     /locus_tag="ACIE68_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFX5906844.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(55482..56246)
                     /gene="pgpB"
                     /locus_tag="ACIE68_000476"
     CDS             complement(55482..56246)
                     /gene="pgpB"
                     /locus_tag="ACIE68_000476"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFX5906845.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            56416..57006
                     /gene="ribA"
                     /locus_tag="ACIE68_000477"
     CDS             56416..57006
                     /gene="ribA"
                     /locus_tag="ACIE68_000477"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFX5906846.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(57070..59745)
                     /gene="acnA"
                     /locus_tag="ACIE68_000478"
     CDS             complement(57070..59745)
                     /gene="acnA"
                     /locus_tag="ACIE68_000478"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFX5906847.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLAAEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(59909..60004)
                     /gene="ymiC"
                     /locus_tag="ACIE68_000479"
     CDS             complement(59909..60004)
                     /gene="ymiC"
                     /locus_tag="ACIE68_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFX5906848.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(60118..60285)
                     /gene="yciX"
                     /locus_tag="ACIE68_000480"
     CDS             complement(60118..60285)
                     /gene="yciX"
                     /locus_tag="ACIE68_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFX5906849.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(60288..60428)
                     /gene="ymiA"
                     /locus_tag="ACIE68_000481"
     CDS             complement(60288..60428)
                     /gene="ymiA"
                     /locus_tag="ACIE68_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFX5906850.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            complement(60747..61721)
                     /gene="cysB"
                     /locus_tag="ACIE68_000482"
     CDS             complement(60747..61721)
                     /gene="cysB"
                     /locus_tag="ACIE68_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFX5906851.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT        14898 a        16272 c        15283 g        15362 t
ORIGIN      
        1 atcgatttta tctgtacgtc cctgattgcg gttattctga cgattaagct atttttgctg
       61 attaatcagt ttgaaaaaca gcagattaaa aaaggtcgcg atatcaccag tgcgcggatt
      121 atgtcgcgca ttatcaaaat caccattatt gtggtgcttg ttctgcttta tggcgaacat
      181 ttcggcatga gcctttctgg cttgctgacc tttggtggta ttggtggtct ggctgtcggt
      241 atggccggta aagatattct gagtaatttc ttttccggga tcatgctcta tttcgaccgt
      301 cctttcagta ttggcgactg gatccgttca ccggacagaa atatcgaagg tacagtagcg
      361 gaaattggct ggcgaattac caaaattacg acctttgata atcgtccatt gtacgtaccg
      421 aactcgctgt tttcgtcgat cagcgtagaa aacccaggac gaatgaccaa ccgccgcatt
      481 accacgacca ttggtttacg ttatgaggat gcggcaaaag tgggcgttat tgtcgaagcc
      541 gtacgtgaga tgctgaaaaa tcacccggcc atcgaccagc gacaaacctt actggtttat
      601 ttcaaccagt ttgccgactc gtcattgaat attatggttt attgctttac caaaaccacg
      661 gtatgggctg aatggcttgc tgcacagcaa gacgtttatt tgaagattat cgatattgta
      721 cagtcacacg gcgcggactt tgccttcccc agccagacgc tgtatatgga taacattaca
      781 ccgccggaac agggtcgcta aatcagacat cactggcgtt atccacaagg gtaacgccag
      841 ttcccatcac atcaatgctt cacaatatac atagtccgac tgtacgccac atcttcaggg
      901 ttattaatgg gataaccttt cagccacggt ttgattaatc gtccattggt atattgataa
      961 attggtgcaa tcggtgcttg ctccatgagg attttttctg ccgcgttgta atcggcatta
     1021 cgcgctttaa cggtattttc cgtcgatgcc tgggccagaa ctttgtcata tgccggattg
     1081 ttaaagcgtg aaatatttcc tgaatgcgtt gacgttaata atgtcaggaa agtggagggt
     1141 tcattataat cccccaccca tgaggcgcgg ataacatcaa aattgccggt gttacggcta
     1201 tcgatatagg ttttccattc ctgattttgc aatttaacat ctacgccaag gttctttttc
     1261 cacatcgatg ctacagcaat cgcaattttt tgatggtttt ctgaagtgtt atacaaaagc
     1321 gtcagcttca gcggtttttg cggaccataa ccagctgcgc tcaacaaagt ttttgcctgc
     1381 gcattcagtt cttcctgact catttgttca aacggcgaag gttccggcgt aaatccggcg
     1441 gtaacatctg gtgtaaaatg ccacgctggc ttttcgcccg tccctaatac tttttcggtc
     1501 atcaggcggc gatctatcgt catacttaat gccagacgaa cgcgctgatc tgccgtcggc
     1561 cctttttgcg tgttaaacgc ataataatag gtcccgagct gcggcggcgt ataaacctgc
     1621 cccggaatat ccttcaacag cttctgatac atatttttcg ggaaggattc ggtgatatca
     1681 atatcccccg cgaggtaacg cttagtggct gcggattcct gattaattgg caggaaggtc
     1741 actttttgca gtaccgtttt ggcgttatcc caataatggg tattcggtac cacgaccagt
     1801 ttttcattga ctacgcgctc tttaagaaca taagcgccat tgccaatcag atttccgggt
     1861 ttcgtccact ctttaccgct ttctacgttg gctttttgca ccgggaagaa ggcaaagtta
     1921 gcggttaaat tcacaaacca cggcaacggt ttatcaagct gaattttcaa agtatgggca
     1981 tcaactgcgg tgacgccaag ctggtcaggc gtagctttac catcaataat cgcctgtgcg
     2041 ttgttgattc ccgccagcgc ggcaaaccat gcaaatggcg atagagtttt cgggtccacc
     2101 agacgttgcc agctgtagac aaaatcttgc gccgttaccg gtgtgccatc cgcccatttt
     2161 gcgttatcgc gcagggtaaa agtccagata cggttgtcat tacttttcca ctgagtcgca
     2221 acgccgggga caatctcccc tttctcgttc tgattcacca gtccttcaaa cagatcgcga
     2281 atgacctgaa tctctggcag gcccacggct ttagcgggat ccagcgacgc aggctcatct
     2341 ttaatgtggc gcaccagctc ctgcttctct gccagtactg tgccgctcgg aacttctgca
     2401 gcatacgaaa gagaaatgct gctgaccaac agcgcacaac acgtgactga aacagagtgc
     2461 ttcataagtt tccctctaac cgacattaaa attgatgcgt aattatttgt ttcgccttaa
     2521 agaaatgcaa acaacttcca tcaaaaaagt gacacaggaa gcataaaatg ttttatgtat
     2581 caatatatta aatcagattt agtgtaaata atcgacgcca aaatgcggta aagcacatag
     2641 aacaccgaac actacgttca ggcttcgcac gttcaaacca gttttcggta tcataatcag
     2701 catctgtcgc cattgtacga agaataaaaa ataaccaaaa cgttgaaagg aactgagaat
     2761 acgttaagag agaaattaaa ttaatgtatc agcaagtagt gctaaacagc acctttatac
     2821 ttcagcctat ctataaataa tgagaatgcc gagccagcat gtcgacggtg tggatagtac
     2881 aggtgataac cgggtaaatc tggtgtgaat ttatccaaaa cacgtatcag ttttttttct
     2941 ttaatagccc gctcaacctg atcataaggt agatacgcca atccatgccc atcaattgca
     3001 gcatcaatga tcaggtctat cgtattcagt aaaagctgac cttccatgcg aacacgaact
     3061 tcacgtccac cgcgtattaa tctccagcga tttgctgtac ccgatgtggg aagatacaaa
     3121 ttaattgcct gatgatctat taattgtgac actgacgttg gaacacttcg gcgagaaaaa
     3181 taatccggtg agccaacaat agccattggt atatctggcc cgattcgaat ggcgatcata
     3241 tctttatcca tctccccacc cagacggacg cctgcatcaa aacgttcaga aacgacatcg
     3301 gtcaaaccat aatcaatggt gagctgaata tcaatttcag gatgcgattt caggaatgtg
     3361 cgcattgctg gtaacaatat cgtttttgct gcatgttcta cagtagtaat acgtattgtc
     3421 ccggatgggc ggttctgcag ctcgctcagg gatgccaggg ctgaatctat gtcatgcaac
     3481 atagggccaa gaacagacaa aagatgctcg cccgcttcag ttggaacaac gctgcgcgtg
     3541 gttcgcgtca gaagccgcaa tcccaatcgt tcttctatac gacgcactat ctggcttaaa
     3601 gctgactgcg ccatgctcag gcgggctgct gcacgagtga agctacgctc ctctgcaacg
     3661 acgacaaacg ccatcagatc agcgatttct tcacgcttca ttcagtatct ccatgcaatc
     3721 tattaatcat ttaaatgata ggtctaatca tgattttggg tctaatcaac ttaatgatca
     3781 atatctatag tgatatcacc aaccgttaaa acgaaccgac aactccatga ggaacggaat
     3841 gatgaataat aaagtcagct tcactaacag caataatcca accatctctt tgtctgcagt
     3901 gatctatttc cctccaaaat ttgatgaaac ccgtcagtat caggcaattg tggtatctca
     3961 cccaggtgtg ggcgttaaag aacaaacggc cggaacctat gccaaaaaac tggcggaaaa
     4021 gggatttgtc actattgctt atgacgcatc ttatcaaggt gaaagtggcg gcgagccgcg
     4081 tcagttagaa aacccataca ttcgtactga agacatcagt gccgtgattg attacctgac
     4141 aacgctttct tatgtcgaca atacccgaat tggtgcgatg ggaatttgtg ccggtgcagg
     4201 atatacagcc aatgctgcaa ttcaggatcg ccgcatcaag gccatcggta ccgttagtgc
     4261 ggtcaatatt ggttcgatgt tccgtaatgg ttgggaaaac aatgtgaaat ctattgatgc
     4321 cctgccatac gttgaagcgg gttcaaacgc ccgaaccagc gatattagca gcggagaata
     4381 tgccgttatg ccactggcac caatgaagga gtccgatgcg ccgaacgaag aactgcgtca
     4441 ggcctgggag tattaccaca cccctcgcgc gcagtatcca acagcaccgg gttacgctac
     4501 tttgcgcagc cttaaccaga ttattaccta tgatgcttac catatggcgg aagtgtacct
     4561 gactcagccg atgcagattg tggcgggcag ccaggcaggg agcaaatgga tgagtgacga
     4621 cctgtacgat cgagcatcaa gccaggataa acgctatcac attgttgaag gcgcaaacca
     4681 tatggatctt tatgacggca aagcctatgt ggctgaagca atttcagtat tagctccgtt
     4741 ctttgaggaa actctgtaat gaaacatatt caaatccgca atagtgatat ggcctggcat
     4801 attgctgcca ataatctggg ataattttca tgaaaaaggt actgattctt ggtgctggcg
     4861 gtcagatagc ccgccatgtg attaatcaac tggcagataa gcaaaccatt aaacaaactt
     4921 tatttgcccg tcagccagca aaaatccaca aaccctatcc aactaataaa atgcaaacaa
     4981 cttccggcaa ataaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata
     5041 atccgcaaaa tttgcacaac actgcctgta atacttcctg aaccagagac gatcatgacc
     5101 gtaacccgcc cacgcgccga acgcggcgca tttccgcccg gaacagaaca ttacggacgt
     5161 tcattattgg gcgcgccgtt aatctggttt cctgcccctg cagccagccg cgaaagtggt
     5221 ttgattctgg ctggcactca cggtgatgaa aactcttcgg tcgtcacgct ctcctgtgct
     5281 ctgcggacgt tgacaccttc tttgcgccgc catcatgtgg tgctgtgtgc gaatcctgac
     5341 ggctgccagc tggggttacg ggccaatgct aatggtgtgg atttaaacag aaactttccg
     5401 gcggcgaact ggaaggaagg tgaaacggtt tatcgctgga acagcgccgc tgaagaacgt
     5461 gatgtcgtat tactgacagg tgacaaacct ggctcagaac ctgaaaccca ggcgctgtgc
     5521 cagcttatac accgcattca acctgcctgg gtggtctcat tccacgatcc actggcctgt
     5581 attgaagatc ccagacacag cgaattaggc gaatggctgg cccaggcgtt tgaattgcct
     5641 ctggtaacca gcgtcggtta tgaaacgcct ggctctttcg gcagttggtg tgcagatctc
     5701 aacttgcatt gcatcaccgc agagtttccg cccatctcct ccgacgaagc cagcgaaaaa
     5761 tatctctttg cgatggcaaa cttgctgcgc tggcatccta aagatgcaat tcgcccgtcg
     5821 tgaactgaag cgccggttcc acatctaccg ccagccaggt cggtccgtca atatcggcga
     5881 aactgacctg cggcaccagc ggtaaagcgg cgctaatggc acgagaggta cacaacatgc
     5941 agcccagcat cagactgaaa ccttgtgcac gcgcttcagt cgccagcgcc agcgcttccg
     6001 tcagaccacc ggttttatcg agcttaatgt taaccatctc atagcgccct ttcagcgcct
     6061 tcagattgct acgtgtatga caactctcat cagcacaaat cggcaacgga tgaataaaat
     6121 tctccagcgc cgcatcgtcc tgcgcaggaa ggggttgttc aagcatcgca acgcctaaat
     6181 ccgccaatag ctggcaacgc gccgccaacc cttctgcacg ccaggattca tttgcatcaa
     6241 cgatcagcgt cgcatcgggc acagctgtgc gaattgccac catccgctca ctaataagat
     6301 ggttatccag cttcactttc agtaatttcg cgcctgcctg ccagagtgtt gatgcactat
     6361 tggccatctg atcaggcgta ccgatgacta ccgtctgtgc agtgataact gtctcgggaa
     6421 gcgttatgcc gatcaaatca gccagcgatt gctgctgttt tcgcgcggcc agatcccaca
     6481 atgcacaatc cagcgcatta cgtgctgcgc cggcagggag aattttttgc aactcctccc
     6541 gtgtcagccc tttctctaat tgcgacacga cgctcataat ttgcgccatt accgaggcat
     6601 cactttctcc ataacgcgga tacggcgtgc attcgccggt gcctttaata ccctcttctt
     6661 ccagttcaac caccaccacg cgcgcttcac tgcgacttcc ccgggcaatc acaaacgggg
     6721 tatgtaaggg ccaggcttcc tcaaatacct taacggttct catctttact ccttgttgtc
     6781 gcggtggtct gcagattaag cgcaaatagc gtttgctgtg ttattgacag ttagcataaa
     6841 ctaggtgtga cgttaactat atgtaaacag gaaagataat catgtcacaa accgttcatt
     6901 tccagggcaa cccggttaca gtcgccaatt ccatcccgca ggcgggtagc aaagcgcaga
     6961 cttttactct cgtggcaaaa gatctgtctg acgtcaccct cggtcagttt gcgggtaaac
     7021 gcaaagtgct gaacattttc ccgagtattg ataccggtgt ttgcgccgca tcagtacgta
     7081 agtttaacca actggcaact gagatcgaca acaccgttgt gctgtgtatc tctgccgatc
     7141 tgccgttcgc ccagtctcgt ttctgcggcg cagaaggtct gaacaacgtt atcaccctct
     7201 ccactttccg taacgctgaa tttctgcaag cttacggtgt ggcaattgct gatggcccac
     7261 tgaaaggtct ggcagcgcgt gccgttgtgg ttattgacga aaatgacaat gtgattttca
     7321 gccagctggt ggatgaaatc accaccgagc cggattacga agcagctctg gctgtactga
     7381 aagcataatt taatatgcct gatggtgttg caccatcagg catattcgcg cttactcttc
     7441 gttcttcttc tgactcagac catattcccg caacttattg gcaatcgcgg tatgtgaaac
     7501 gccgagacgt tttgccagtt tgcgcgtgct gggataattg cgataaagct gggttaatac
     7561 cgagcgttca aaacggctgg tgatttcgtc cagcgaacct tccatcgcat cttcgcccac
     7621 ggctaccgtt gcggcgtcat aatccggcaa caaaatatcc tgtggacgca gctcataacc
     7681 gtccagttgt gtcagtgcgc gatagatagc gttctttaac tgccgcacat ttcccggcca
     7741 cgcataacgc gtaagtacag tattcaggtc agcggccagt ttcggacgcg gcacgccctg
     7801 ctcgtcggca aagcgggcga cgaacagctc agttaacggc atgatgtcct gcggacagtc
     7861 acgtagcggc ggcagattga gcgtcaacac gttcagacga taatagagat cttcacggaa
     7921 catgcctttt tgcaccagtt cgaccagatt cttctgcgta gcgcaaatca cccgcacatc
     7981 gacatgcacc tcatggtctt cgccaacccg acggaaagtg ccatcattaa ggaaacgcag
     8041 taatttcgcc tgcatccgtg gtgacatttc ccctatttca tccaacagca ccgaaccacc
     8101 gttcgcctgc tcaaagaatc ctttcttccc ttccggagca tgaccaaaca gttcactctc
     8161 gaccgcatct tccggtatag acgcacagtt cagcgccagg taaggtttgc ccgctctggg
     8221 gcttgcctga tggcaggcgt aggcaaagag atctttacct gtacctgtgt cacccgtaat
     8281 cagcagcggc gcgcttagca tcgccagttt ctgcgcctgt tcgacaacat gcttcatttt
     8341 cgggctgacg gcgacaattt gactgaaggc gctgacgtcc tgggcggcga cattttgcaa
     8401 ctggcggccc atacgaatcg ttgatcgcaa catcaccacc gcaccggtca ggacgtgttg
     8461 atcattttca tcctgaagat aaacaggcgt aatctccatc aggaaattct gcccattaat
     8521 aacgacatgc tcgttatgcg aatcttgcgg ttcgctttcc agccaacgta aaaaattaaa
     8581 gccgttaatc aattgtgcgg cggtatggtt gcgcaggcga tccaattttt gcccaaaaag
     8641 ctgacagctc gccgggttcg ccatatccac tttgcttttc atatcgacag agagcacagg
     8701 ttcaggcaac gcctccagta acgcgctcaa cgccagatgc tcacgttcgg aaggcatcca
     8761 cgggacagta cgcacatcgg taacacccgc aatacggcgt atttcggcca tcagactgct
     8821 gaaactctca aactccagtt cagcaaaatt gaggtagatt cgcccaatgg gatcaatctc
     8881 aataccgcgt aaatcaatgc ctcttagcac gagtagatcg agtaattcgc gggtcagacc
     8941 gagtcggtct tcacaaaaga cttccagacg catgggaacc ttcacctgaa aaaagaacaa
     9001 ttaatatgat gatatgacac tatttggata gcaggaaggt ttctgtcaac aatcattgac
     9061 acaaagacgg aaaaacgggc acttcggcgt aaagatatcc ggtcagcacc cgatattgca
     9121 ttatttttcg ctgggcgttt tgcctttttg cagcgtttct ttcacctgac cgataagctg
     9181 acgacggaaa tccccgaggc gaggtttgtc atcgtcaatc cacggcagcg ggcggcaaag
     9241 ctccatagct ttaatcccga gtcgtgccgt cagaagtcct gcaccaatcc cctgagctgc
     9301 gcgggtagac aaacgagcag cgagatcttg cgacatccag tccatcccca cttcgcgcac
     9361 cagttcgctg gctccggcaa aagcgatatt cagcaatacc agcttaaaca gacgcaaacg
     9421 gctgtaatac cccagttcaa tgccatacag cgtggcgatg cgattaatta aacgcagatt
     9481 gcgccaggcg ataaacgcca tatcgaccaa cgccagcggg ctgaccgcaa tcatcaacgt
     9541 tgattccgcc gccgaacggc tgatttcgcg ccgcgcctgg gcatctaaaa ctggctggac
     9601 caaatgcgca tacaggctga ccacttcacg gtcgttttgc gtttcatgga ttgaggcata
     9661 ccagcgttgc agcgccggat gcgactgatc aatacccgcc tgctgcgcca gtttttcgca
     9721 aaatgcgcgg cctttgcccg tgccgtggct atgcaataaa tcacgcgctt cgtcgcgttc
     9781 atgggcgcgc tgtcgcaagc gccataagcg ccgccactct gttaccacag aacctacgcc
     9841 agcgccgata atcaatgccc cagcggcaca tccacccagc gccacccagt cctgggtttg
     9901 ccaggcattc attgtccact gtacaccctg cccgacaacg cttgcgccaa acagagccag
     9961 cccgcccatc accattttgc gccacaggct gcgtttcgga cgtaacgctg cgtccattac
    10021 cgcttcgact tgcccctctt cttcctgcgc ttcgtcgagc gtggccgggg caaaattttg
    10081 cgcctgattt tcgtcaaagg tctgctgcgc cctgaattta ggattctgat cgacctccag
    10141 aggaccgtcg aaatcaatac gtggttttaa cggttcggtc atcgcaattt atctcctatt
    10201 aaaaattcca gcgcagcatc aagacgaata tgcggtagtg gtttgtcgac atccatcacc
    10261 tgcggacgaa atgcctcaaa ctggaagcct tgcttatccc agaacgcctg accaggcaaa
    10321 cgtgcgggaa cttcgccagg ataaacagtg agcggtgcgc catcgctaag tcgattacca
    10381 cgcagcgccg ggattttctc accgttaaca tcaataatgc cgctggtggt cgcctgaact
    10441 gacgccagcc ccaggcagtc catgctgatc ccttcgaacg ccgcattttg ccaggcatcc
    10501 tgaatcagtt gttgcagtaa tgaaaccata ttagcgtgct gatcgatggt cacatggtcc
    10561 gctttagtgg cagcaaacaa tagcttatcg ataaccggcg aaaacaaacg ccggaacagg
    10621 gtacgctgcc cgtagtgaaa actttgcatc agctgcgtca gtgccagacg catatcatta
    10681 aatgcctgtg gcccactgtt gagaggttgc aggcaatcca ccagcacaat ctggcggtca
    10741 aagcgtagaa aatgattctt atagaacccc ttcaccacct tctcgcagta ataattaaag
    10801 cgctcgcgca gcattccggc attggtatgc ttatcggcct gcgccagttt ggactcgccc
    10861 caggtatcga catccggcca cgggaagaat tgcagcgcgg gcgcacctgc catatctccc
    10921 ggcaagacaa agcgcccagg ctgaataaag tgcagcccct gctgtttaca gtggtggaga
    10981 taatcggtcc acgcggcggc aatgtccgcc agccggtttt cgtcggcagg cgctagcggg
    11041 tcaagtcctt cgcacatcat tcgccatttc gccgaccatt cgccgcgctg accattgagt
    11101 aagcccgtca tctggcgcga ccagcttaag taatcctgcg ccagcatcgg caggtcgagc
    11161 aaccattcgc cgggataatc cacaatctcc agatacagcg tggaggtgtc cttgaagtgg
    11221 cgtagcagcg aatcgttcga tttaaaacgt agcgccaggc ggatttcact gacgccgcgc
    11281 gttggcgtcg gccaggcggg aggatcgcca tataactgcg ccagtccttc atcatatgtg
    11341 aagcgcggaa tgccaaagtc acgctgagga atgcgtttta cgcccagcag gcgctcttca
    11401 cgcaccgcgc ttaacagcgg caagcgtgct ccggcgtgaa tattgagcaa ctgattgacc
    11461 atcgcagtga taaacgctgt tttgccgctg cggcttagtc cggttacggc gaggcgcaga
    11521 tgtctgtcga caccccgatt caccagcgca ttaagttcat ttttaagtcg cttcattgct
    11581 gtcctgtgct gctctgaaga ccagaaaaaa atcattcgta aggatatgaa tgtgggggcc
    11641 aggccattga ttatcaactg cccgtgttgt catcgacggg cgaactccat aagatgtgca
    11701 gcattcacgt tcggacggag ttcctgacct atgtccccta ctatttatga tattgccagg
    11761 gttgcaggcg tatcaaaatc caccgtatca cgcgtgctga ataagcaaac taatatctcc
    11821 ccggaagcgc gcgaaaaagt gttacgggcc attgaagaat tacagtatca gccaaacaag
    11881 ctggcccgcg cgctgacctc ttcgggtttt gatgccatta tggtgatttc tacccgttcg
    11941 accaaaacta cggcgggtaa tccgtttttc tctgaagttt tgcatgccat caccgccaaa
    12001 gctgaagagg aaggtttcga cgtgatattg cagacgtcgc acaatccggc agaagactta
    12061 caaaaatgtg aaagcaaaat taagcagaaa atgattaaag gcattattat gctaagttcg
    12121 ccagcggatg agtcattttt tgcccaactt gataaatacg atattcctgt cgtggtgatt
    12181 ggcaaagttg aaggtcaata tgcccatgtt tattctgtcg ataccgataa ttttggcgac
    12241 agcattgcgt tgaccgatgc attaattgaa agtgggcatc aaaatattgc ctgcctgcat
    12301 gctccgcttg atgtccatgt ttcagtggat cgggtaaatg gttataagca gagcctggct
    12361 gcgcataata ttgcagtgcg tgatgaatgg attgttgacg gcggttatac ccatgaaaca
    12421 gcactgcaag ccgcgcggga attattaagc cagtcaccgt tgcctgaggc agtgtttgct
    12481 actgacagcc tgaaattaat gagtatttat cgtgcggcag cagagaaaaa tattgctatt
    12541 ccgcagcagt tagcggtggt gggttatagt aatgaaacgc tgtcgtttat tttaacgcct
    12601 gcgccaggcg gtattgatat cccaacacag gagttaggtc aacaaagctg tgagttatta
    12661 ttccgcttaa tttctggaaa accgtcacca cagaatatta ccgttgccac gcatatgacg
    12721 ctgaaataaa taccagctac cttgcctgaa tgccggatgc gatgctagcg catcttatcc
    12781 ggcctacatt tatagttcgg ataaagtact cgtgccgcat ccggcaattt agcccattat
    12841 cagaacgagt aatttacgcc gacacctgca taatggaatt tatcgctgtc gccttcgtcg
    12901 tgatcctgcc actcaaacgc gtattccagc gaaacggaaa gcccgttctg gaaatcataa
    12961 ccgtaaaata aacctacacg gttaaaatca tggccttcac gttcaatatc atcctgccag
    13021 tcccagttac tccagcgatc cagcccaatg cgcgtatacg gcgtcaccga gtggttgccg
    13081 agcgtcagcg gcaaataggc gcgaatttct tgcgtagaaa actcaccgtt attgcggctg
    13141 tcgtccatat taaagccgcg ctcgagataa tagttcactc gcaaggcaac cgtttcgttg
    13201 aaggtatatt gcaggcctgt ttccgtttcg actcgggtat cagcgtaacc ggtcgtgttc
    13261 agatcgttgg caaatttata catcgataac caaccgttga aacgtaaatc gtccgtcagt
    13321 ttcacatccc agtctggcgc gattttccag cgctgcatat tcgccgtgtc tttacccggt
    13381 tcatcaacgt agtgataacc ataattacgg aaaccaccgg tcaggccgaa actgaaatca
    13441 tcgttttcga ggaactgata atgcacctcc agctccgggc gatcaaacca cgttccacgt
    13501 ttacccgcgc tataatctac cggcccttcc tgataatagg ccagagcaat tctccacggc
    13561 ccgttggcgg cattaaaata gactgaaggc tccgccagcc catccatatc ttcgccataa
    13621 ccctcgacgt tttctatttc gtacatcgcg ccgatattaa agtgccagtc gttcttttcc
    13681 tcggcctgtg cgcaggccat tcccgcacac atcaccagtg cggtacaggg taataacttt
    13741 ttcattattg ttccttgtat tatcctgggg gaatgccttc cccctgtatt tagcgaagtg
    13801 ctatttccgt ttctgcatca aagaaatgac atttcgtcat atcaaaatga atagtgatat
    13861 tttctcctgc atgataatca tttaacgcac cagcacggac cactaactcg tgccccccaa
    13921 ccgtggtgta gagcataaat tccgcaccgg ttaattctgc cacgctaatt ttggcggaaa
    13981 tgttattttc ctcttgcgcg tccggatgaa tatcttccgg acgtattccc atcacgatgg
    14041 gcttatgcaa acttccctgt gttttcagaa ccgctaattt ctcttcggga atggttaatt
    14101 taagcgtttc cgtaacgaat ttatcgccat cgatcgtgcc acgaataaaa ttcatcgccg
    14161 gtgatccaat aaatccggca acaaacatat tcgctggttg gttataaacc gttttcggcg
    14221 caccaacttg ctgaacaatc ccatccttca taatcacaat ccgcgtcgcc atggtcatcg
    14281 cttcggtctg atcgtgggtc acgtagatca tggtggtatt cagcttctgg tgcagcttgc
    14341 tgatctctgc gcgcatttgc acgcgcagct tggcatcaag gttagagagc ggttcatcca
    14401 ttaaaaacac gcccgcttcg cgcacaatcg cccgcccgag cgccacacgc tggcgttgcc
    14461 cgccggaaag cgcgcccggc ttgcgtttca ggtactcacg caggccgaga atttgcgccg
    14521 cccagttcac ccgctcatca atcacctctt tggcgatttt ttgcatcttc aggccaaacg
    14581 ccatgttgtc gtaaaccgtc atatgcggat acaacgcgta gttctggaac accatcgcta
    14641 tattgcgtgc tttggctgga acgtcattca ttcgtttgcc gtcgatcaac agatcgccgc
    14701 cgctgatctc ctcaagcccg gcaatcatgc gcagggtggt cgacttaccg cagcccgacg
    14761 ggccgacaaa cacgatgaac tctttatcgg caatttccag gttgaagtcc ttcaccacat
    14821 gcacctggtt atcgtagatt ttttgaatat gttgtaacga aagctgagcc atgttgattc
    14881 ctttgctata cgttttgcca gaaggccgat aaccgcggcc aggtgagtga ttccgttgaa
    14941 ggcaacagta attgcgcccc ggttaagccc gcgccgatcc ccaccgagcg cataccgctg
    15001 gcgttaatgg cgtcaatgcc cgcctgcgca tcttcaatgc cgatacatgc ctgcggcggc
    15061 acgcccagcc cggcacaggc ggcgagaaag atttccgggt ccggtttcga gtttttaagt
    15121 tgggaagcat ccgcgcagaa ggtgaaaaac tcgcgcagct ccagcgccgc taaaatcgtc
    15181 ggcgcattca gggagacaga agccagccca accgggatct gctgtgcacg gagatctgcc
    15241 agcaaagagc gaatgccggg tagaacagcg ctgaccgtca actcgcgcag tgagtggaca
    15301 tagagcagat ttttgcgata cgccagttgc gccctctcct gcgagttaaa gtcgccctct
    15361 ttgcccccgt gttgcagaat gcgccgcaga gactcatcgc ggctgatccc ttttagggat
    15421 tcgttaaact gcgcatcaat gctgatgcca atttcagcgg caatctgctg ccacgcctgg
    15481 aaatgcagat gcgcggtatc ggtgattaca ccatccagat cgaaaattac cccttgcagt
    15541 ttcattcatc ctcctgatgt gtggtagcgg tcccattgaa gggcaaaata aaatcaccca
    15601 aacagaaaac agattcttca gccacggata taagctgacc gttcaaacgc agtgaaacgg
    15661 gcgcagaagt tcgaatcgca atacgctgcg cgtcaagagt gacctgtaat tcgcagccct
    15721 gccagaacag agggaaagac aactgttgcc actgctcagg taacgccgga ttgagatgca
    15781 attcaccgtc acgcacgctc accccggcaa aaccctgaat cgcccccagc cagatagcgc
    15841 cagttgcggc agcatggata ccatcatcac aactatgcgg atcagcacca agatcgattt
    15901 cagtcccctc gcgccagaac tgataacttt gggtcagcag gccacagcgt gcggcaacaa
    15961 tgccgtggat tgctttactt aatgacgagt cgtgaatagt gcgcggttca taaaattgca
    16021 gattggcaag acacgatgcc gctgagaact gctccggcag catgtaattg agcatcacca
    16081 catcagcttg tttgaggatt tgcatctcgt tcacttctgc gcgtgaataa tccagcagaa
    16141 tggtttgctt ccccgccgcc gctttgtatt tcgccagatt aatcgccggc ttagccataa
    16201 acgaatcatc ctgcggcaaa acgccgtcgg gctgaatttc tggcagccgc agttctttaa
    16261 ggaacatttc ggcgcgatgg ataaacgcat cgtcgctaca gccaaactgg cgggcaatac
    16321 tcagcgcctg ctggacgttg taatacgcca tatagctggt gaaggcgtta ttattgacat
    16381 gttcggtata ttcgtcaggc ccaataacat catgaatttc cagacggtcg ttaaccctca
    16441 ccgcgcggct aatccagaac tttgcagttt ccagaagtag cgccatgcct tcatgcgcaa
    16501 tgaaactttc atcccccgtg gtctgccagt attgaataac cgcccaggcg atatcggcca
    16561 ccagatgatg ttccgcctgc gccgaggcca ctttttgccg cagcccggtg cgaatgttaa
    16621 tggcggcaaa ttccggcgtc tcttcttcgc cgctgcgcgc gctttcccac ggaaatagcg
    16681 cgccctgcca gccgttgcgt cgcgctttct cctgcgcgcc tggcaagttg tgccagcgat
    16741 aacgcagtaa acttcgggca accgtcggat cgctaaacag atgaaacggt aacaaaaata
    16801 cttctgtatc ccagaaaacg tggcctttgt agccttcgcc ggttaagcct tttgccgcaa
    16861 tgctgctgcg ctcgtcgtgg gcaggcgtca tgatgcgcag atgataaagc gcataatcta
    16921 acgcttgctg atcgtgcgct tcgccgccat ttaccgtgat acgacgtttc tgccaccatt
    16981 gacgccagtt ttctgttgat actgcaagaa gttggtcgta actctgctgc gcgcacattt
    17041 caagctggcg aagcgacgcg ctgccccact cgtctaaaac ggcttgcctg tcatcccgcc
    17101 agtcgatcca caccagtttt tgcaacgtca ctgtctcgcc tgcatgaagc tgcgcactgg
    17161 tatgttgctg caaacggcgc tctttggcgg tatagcattg ctgcacatca ccgctcacca
    17221 tacagcaaca gctgatggcc acatcactgc gtccatcctg ggtggtgtag atcccctgca
    17281 tcagatgctg accaaacacc cgcacctggg tttcgtcgag atgttggcga ccgtggttgg
    17341 tttgcgtggc gtcgatacct gttgaaatca gcactgacgc gtcggcgtcc agtggcgtaa
    17401 tagtgatttc cagcacaatg agcggcagtt ggtctgccga aacaaagcga cggctggcga
    17461 tggtgtaacc tgtgccgttg ctggtacgcc agacaacgct acggcgtaat tctccactgg
    17521 caaagtcaag ttcacgctgc caggcttcgc gggataacga gaaaacctca ccatttatgg
    17581 caatctccat ccccaagata tcaggcaggt tcaccagttc gttgatttca ccttttcccg
    17641 cccgatgata cagccccgcc agatacatcc ctcgcgtctg gcgcgtgtaa tcttcttcat
    17701 ggctggcgcg aagcccaaga tagccgttcc cctgcgccat cagcgatgca tacttgttca
    17761 gggtatgctg gctgaaatgg ggttctgata acgttactgg cctggtcata attccacacg
    17821 cgtccctgtt tctgcggatt gatacaacgc cgccaccagt tgctggatga tgtacccctg
    17881 ctcggcgtcg gctatcatca cgggcttgcc ctgtacgtga ttgataaagg cttccatgct
    17941 gcgcaaatgg cggttgtcgt ctgccagttc ccgttgcatc agcgtcatta attcaccgtt
    18001 gttatcggtg tagatatgtg ctggaaacag cgtcgcaccg gctttatcac cacaaaagct
    18061 gacgttcata atcgactgtt cgcggatgtt gagtgcaaat gacgtttcca gccacagaat
    18121 gccgccgtta tgaaattcaa ttgtgccaaa cagcgaatct tcgacgctat aagttgccgg
    18181 atcccactcg ccaaattgac cacagctctt ttgcgtgccg atcttttgaa agctatgcgc
    18241 attcacgctt ttcaccgccg gaaagcccag cacatacatc gcagcatcca gcatatgaat
    18301 gccgatgtcg atcagcgggc caccaccctg cagttcttta ttggtaaaga cgccccagcc
    18361 aggaacgccg cagcgacgca gggcgcgggc ggtggtaacg taaatctctc ccaaaacgcc
    18421 gttggtcacc tggtcacgca gctgttgcgt atcgagcgca aaacgatggt gaaagtcgta
    18481 ggccagcacc ttgcccagtt tgcgcgcggt atcgcacatt tcccgcgcct gttctggcgt
    18541 catggcgggc ggtttttcgc acatcacatg gcagcccgct tccagtgcca tcagggtatg
    18601 ttcgtaatga aaacggttag gtgagcagac gctaaccaca tcaggtttca ccgccagcag
    18661 catggcctgc ggatcgtccc acacggatgc attcccgtat ttttctgcca gcgcctgcgc
    18721 ctgggaaagg cggctgtcac agacagccac cagttccaga tcgttgcggg tgcagtagta
    18781 cgaagcatga accttatccg ccacctggcc tgcgcctatt atcgcgaccc gcagcggaga
    18841 gcttgtcatt gcacttttca caaagacctc ttagcaggta cgcaaccagg ccaacgaatc
    18901 acggtacgcc tgggcaggat cttccgcccg gatacgccct tcgtacacta catagccctg
    18961 atagttatcc gcgcgcagct gttcaaacag cgcgtggaaa tccagggtgc cgctgcccgg
    19021 ctggtagcga tggttatccg caatatgcac atgaccgagc aggtcgcggt tgtcatgcag
    19081 cgcctgcgcc aggttatctt cttcgatatt catgtgatag aaatcgccga taatctgtac
    19141 atgtttaaga tcgttttcga cgatgtaacg gcgggcatcg gcgagggtgt tgatcatatg
    19201 atcctgatag cggtttaacg gttcgagata caccacggtt ccggtacgcg cggcgacctg
    19261 ttccagtacg cgcagggaat cactcaccat tttgcggtcg ccgtccaggc tacgcggcga
    19321 ggtcatcggc ggtaagcgga aggtaaacat gccccacgct gccggaacga cgataccttt
    19381 accccccact tctgccagcg cttcgagaat gcgttcgatc tgctttaagc cattaagacg
    19441 acgctcttcg ataaagtcgc caatccaacc gtcatagcca ccgcaggcgg tggtcaccgg
    19501 taaaccggtt tctttgattg ctgctttgac ttcttcgaga ttgttgacca gcagtttgcc
    19561 gtcaatctca aaaccatcga agcccatctc tttgatataa cgaaatttct ccagaatgtt
    19621 ttccggaaag aatgcctgat tctgtgtgcc tattttcatt ttttcattcc tttgaattaa
    19681 aacgtaacgc ccattttgat gctctgttcc ggatgctgat cgacatactg catgtagctt
    19741 tccgggctat tggcaaaggt cactaccggg tcgattaaat cttcgcaatt gagataaccg
    19801 ttcatcagca gttcccagca ggtttcttca atacgcttac ggctccagcg cggataatcc
    19861 gggttcggtt cgctgcacgc gcgggagaaa acaattttgg cgttattgaa atgcgcttcg
    19921 cgtccgaggt taaaaccttc agcaaatggt ttggcaaacg cgacataaga gatggtgccg
    19981 ccataagcaa ggccacgcag cgccgattgc agcgcgtcgg cgtaaccgct ggtttcgata
    20041 atcacatcgg caccctgctt gccggtcagc gttttgatct cttttcctac gtcagtgcca
    20101 atggggttaa ggcagaaatc agcaccgtgg cgacgggcaa tatcacagcg ataggcgata
    20161 ggatcgacgc caatcaccac cgacgcgcca gcgcgtttcg ccagttggat ggctatttga
    20221 ccaatcgcgc caagccctac caccaccaca aaatcaccca cgcgcacgtt ggcatcgcgc
    20281 acgccgctca tggcaaactg cgccgggtcg taacaaacgg cgtttttcca cgagctgcct
    20341 tgcggcattt tgcgcagctt gtagttattc accgcgttaa tgatgaccgt ctcggagagc
    20401 gggccgtagc cgcatacgct gtcgcccacc gcgtagtcgg taacgtcgct gccgcactcg
    20461 ataatgtcgc caaccaccat gttgccaagc tggaatttgc caaactcaat gccgcgcggc
    20521 gcatctgccg gacgcggagt gaacatctgc cattcgccgt taaagtcttc atcgataaac
    20581 gggctggcgg cgcggaagtc gaccacttcc gttccgtgtt tcggtgcgcc gaaacgcacg
    20641 cggattttca cttcattggc taaaatcgcc cgatcttcat actcaaccag cgcagcaaca
    20701 cgcggtgctg tggctactaa ctttttcatt taaaactcct tgtaaagaaa ctggccgctt
    20761 tagcccttca cgccaccggc ggtcaaacca ctcttaataa aacgttccga aagcgcatac
    20821 ataatcacca ccggcaatgc ggtcaccagt gaggccgcca tcatccgtcc ccagatgtaa
    20881 tctggcgtac tgaacagcgc gttcaggccc accggtaagg tgaaattgct ggcgctggag
    20941 aggaagatcg acgcaaacag atagtcgttc cacgccacca taaagcagta gacgaacacg
    21001 gatatcagcc cggacatcgc caacggcacg gtgatgcgga agataatttg cagacggttg
    21061 aggccatcca tcatcgccgc ttcttcgatc tcatcgggga tggtgtcgaa gtagcttttc
    21121 agcataaaca cggcggtggg cagagtttgc gtcaccatgg tgatgatcag cgccatctca
    21181 gtgtcgtaaa tgcccagcgc ggtgatgatt ttgaacagcg gcaccaccag caaaatgcca
    21241 gagaacatat acaccgtgta aaagctggcg ttgatggtca tccgaccttt aaagcgcagg
    21301 cgggaaagcg cgtaagcccc aagaatgccg aggaataccg ccaccacgga tgaaaccaca
    21361 gacaccacca gactgttacg gaagtagtcg acaaacggga aaatcatcgg gttaaaaatg
    21421 tcgacgtaat gctccagcgt ccactgctgc ggcagcagcg taggatgcag tgagatcgcc
    21481 tctttcgcgc ccttgaacga ggtcatcagc atcacaaaaa atggaaacag cgtgatgatg
    21541 agaaacagcg ccagcccgca gtaaaaaccg atgcgactga gtgtgcgttt atttgttgcc
    21601 attgaggttc acccgtttcc tggtcagcag aatgacagcg aaaataatga tgaagagcac
    21661 caccgaaatc gccgccgctt tccctaagtc attaaaggca aaggcggttt tgtagagata
    21721 gacgccgaga atatcgacct tggtggtcag caaataaaca tccgcgaaca tgtagaacat
    21781 ccagatggtg cgcagggtca ccaccgtcgc caggaccggc ataatcgcgg gcagcgtgac
    21841 gatacgaaac cgttgccagg cattagcgcc atccatctct gccgcttcgt acagcgattt
    21901 gtcgatggtc tgcaaaatcg ccagaaacga gataaaggca tacgggaagt agcgccagat
    21961 ggcgaacagc accaccagca caaagctact gcccggatta tcgaaccaca gcggtgcctg
    22021 ctcatagaga tgcagaagat cgacgccgag gtagttcaca atgccgtagc cgttgttgaa
    22081 catgtatttc caggcgaaca ccagcgaaat ggacggcgtt acgtaggaga ggatcaccag
    22141 cgaacgcgcg gttttgcgca ggcggaactc gcgattgaaa aacatcgcca ccgccagccc
    22201 gagcacggtg ctgcccgcca ccaccagcgc ggtgtaccag accgtcatcc acagtgagtg
    22261 ccagaagccg ggatcggaga ggatacgcac atagttgctc accccaacaa aggttgactc
    22321 gatgttggga ttgagcggca gacgtaaaaa actaatttcg atattcgaca ccatcggcca
    22381 cgccaccaga ccgcccagca gtaataagct gggcgcgaga agcaacagcg caaagggcat
    22441 atcggaacga cctgaaaaaa gcctgttcat tgcggattcc tgtcctgggt ttagtgctgt
    22501 tcgaccagtt catccagctt tttctggctc gcttgcagcg tgccagggag atcggcttta
    22561 cccacggtga cgttatgcac catggagctc accacgccag accccgtcac atcacccatg
    22621 cgggtaaagt ttttatcccc tactgcacca aagacctgaa tatttggcag ttcaccgatt
    22681 agctgattcg gtagttcacc cagcgcctta ataacgtcgt tgtctttcca ggtggcggta
    22741 gtgacaaccg ctttattcac cggcagcgca gcacctggcg acatcatcac ccagtcggca
    22801 atgttgtctg cctgctccat aaaggtgaca aatttttctg ctgcttccgt ctcttcggtc
    22861 ttttgcccgg cggtaatggt cagcgaggtc aacatgccgt agaccgcaga gtttttctcg
    22921 gttggcacca cgaaaccgac gtttttcggg tcgccttctt taatcacagc cggaaggata
    22981 taggtggagt aaatcgccat cggcgcggtg ccgttcataa aggcgtcttt cacttccatg
    23041 atgtcgttag aacccggcat agtgttggca gcaaggtcgc ggtaataggt cagtgcctgc
    23101 attatctctg gtgtatcaag ggtgattttg ccttcggcgt taaagacgtt agcctggttg
    23161 gataacgcaa actgggagaa ggattgttcc gtcaacacgc tttctgctgt aggcagcgca
    23221 atgccgtatt ttttattcgc cgggtcattc agtttctgtg caacgtccag cagctgttgc
    23281 cagtttttcg gctcctcaag ccccgctttt gccagcacat ctttgcgata ccagataccg
    23341 ccaatccagg cgctgacagg aacaccggtc catgcgctac catcttcggt acgcacaata
    23401 cgcagtacgc catcgtaaaa cgcgccttca ccaacgttgc tgatgactgt ggcaaccgct
    23461 ttgcgatcga taagctgctc tttgtccatc actttggcgt agtcatggct ggtttcgatc
    23521 acttccggca gcgaaccgct acgtgaaaga gtaatgactt tagtgttata ggcatcttct
    23581 tccacgggca cttgcttaac ggtgatgcca gggttttctt tttcaaaacg ggcaatcaat
    23641 ttactgataa cggcctggcg ctcctgctcc accgaagaat gcataaattc gatggataca
    23701 ttcgttttat tttcttcttt acagcctgaa atcagggcgc aggaaaccag tgctgataac
    23761 agcacgattt ttgatttaat catgagatgt tcctttttat ttaatccaca taacctgcca
    23821 ggggcgcagc gttatttcat tacccaatat atcaacttca ctaatcagat cccgaccatg
    23881 cagattagta atatttacat gctgaatatt tttactgaca ttaaacaatc cagtcagaca
    23941 attaccatca gcgttacttc tttgaatacg cattacggat gaattaatcg tatcaatggt
    24001 aaaattatta tccggatgaa actcgttatg gctgcgacga agtgtaatta aacgcgacaa
    24061 ctcatgatat accgcatgcc ttaatgtagc ttcatcgttc agttctcggg ttatctcttt
    24121 actgtgatat tttttacggt taatcgcacg gttatatccg agtttttcga cacctgcgta
    24181 atcattacgc gagccaagaa tactttgaat atatatcgct ggcacaccgg ggaaacttaa
    24241 caaaatcgca tgggcgagaa taaacctggc gcaacgttct taatcgctac tctcacggcg
    24301 gcttaacgca tccatatagg tcacatttat ttcatacgga ctgcgtgtac cgtcgggatt
    24361 atttttccag tttactaatg caccttcctg ctgtaacgcc tcgaccagct ctaatatttc
    24421 gctttcaggc aacaagcccc gtagcgggtt tagcccgatg ccatcgtgag aggcgaggaa
    24481 gttaaaccag gtggtgttgc tggaaggtag tgtcaggttt tgcgcccacg cacaaagcgc
    24541 ctcaacgttc tgtttttgca ccgcatgcag caccagtggc ggcagcgaga actggtacac
    24601 catatgtgct tcgtcatcgc ctgcgccaaa gtaagcaatg ttgtctttat gcggaacatt
    24661 ggtctcggta atgatcactg tacctggcgc aacgttatca ataatcgacc gtaacagttt
    24721 gataatcaga tgtgtttttt ccagatggat gcagcttgtt cccggctctt tccacataaa
    24781 gccaacggca tccaggcgga catactcagc acctttcgca aggtaacaga gtaaaacatc
    24841 caccatcgcc agcaacactt cagggctacg gtaattcagg tcaatttgat cgtcactaaa
    24901 ggtggtccat aaatggcgcg ttgaatgatc gcgcatctgg aatggcgtta ataacggtaa
    24961 cgcacgcggg cgagtgacgg cgctgagatc ggtttgcggg tcaacggcaa taaaaaaatc
    25021 ttcaaaacct ggatgctgtt gtaaatagtt tttaaaccat tcactttttg ccgacatatg
    25081 gttgcagaca aaatcaaaca ttaaatgact gcattcaccg agttgctgaa tatcctgcca
    25141 ctcccccgct tcactggcga cctgttgata atcaattacc gaaaagccat catcagatga
    25201 ccatggataa aacggcaaca aatgaacatg tgaaaaaatg ctttgcagcc attgatggta
    25261 aaactgatta aatgtgggta atggttttaa atcattgctg tgaaattgat cggcataggt
    25321 aattaacacg acatcacttt catcccagtg ttttttacgt cgctgtgtaa ttaatcgttt
    25381 cgcactctca agacgcgtta caagtttctg taaatgagtt gcggtaaatg ttccaccgta
    25441 gatttcgtcc aggtgatccg taattttctg tttcatgttg cctttccgtg gtagcggtcc
    25501 catgaaacga gagactactc cgcagaaaat aaactgtcaa ttgagcacaa gggttttagt
    25561 cacgcaggaa atcatttttt accagggaaa aagcgtattt gtgagctgac gcaaataatc
    25621 tatggggatg taaataaggt gtgacagaaa aaaaaacggc gcataagcgc cgctcatggt
    25681 gaattctttt aacctttgac cttcggcatt gcgatgtctt tcaggccacc ggcgttctca
    25741 acgtgggtat atcccatctc gctaaggatc tcttttgctt gccctgactg gcgtccggca
    25801 ttgcaataca ctttcacggt gtcgttttta tccggaacgg cggtggcaat gcgctctttc
    25861 acttccttca ggggaatatt gatggccccc tgaacgtgct cttgctgata ctgctctgga
    25921 acacgaacat cgatccagtg ttcagcggcg aaaacgggca gtgaaaacac cagtgccaga
    25981 gcaagtaagc cttttttaaa catgtaagcg tccttctatg gatgaaaatt gccatccatt
    26041 ataggcctga gatttaacaa tttttgcgaa actcccctca ccttttataa cgctgtgcca
    26101 gtttattagc agcccgactt aacagcggtt ccagcgccac agccagcaac attttcagcg
    26161 gtcggcgagc aactgatttt atcgcccagc ccgctacacc cgccgggcca tagcgcagtg
    26221 cggtaagaag taccagcttg cctgctaatt tgaaaccagg ctttaccttt tgcccggcct
    26281 gttgccagcg agtattcata actttcctca cagttgacgg aaacggctac gtaacgtgaa
    26341 agtatcggaa gtgacataac gttccatctc gcgtaaacgc gtttcacttg ccgccagttc
    26401 acggtcgact tcatccagca attcgctgct ggaaggtagc tgctcaccaa aggccatgtt
    26461 gtccggcatt ggatcaagcg caaatgacaa aatgatgtaa gcaaccaggg taaacagcgc
    26521 cagaccgaag aaaatcgaca gcaccaccag gatacgcacc agttttaccg gtacatcaaa
    26581 atagttggca atcccggcgc agacgccgcg gaccatgccc tgctgtggaa tacgccataa
    26641 ttttttattg agattaatgc ccgccattag cgatccctcc agttcggatg ttctgcatca
    26701 agaatggatt ccagcgcctg aatacgttcg cgcatccgtt ttgcttcatc agccagttgc
    26761 gctaatcgct gctgctcact ttgcgacaat tcactgcgac cagaacgatt gctgtaatgt
    26821 agccataacc agatcggtaa aacaaacagc acaaaaatgg ttaacggaat agccagaaat
    26881 agcgcgctca taagtactcc ttagacgatg agcggcggcg cgttcggacg ccgcctggat
    26941 gttattattg attgtcttgc ttcattttgg cttttaattg tgccagttgc tcgcttattg
    27001 catcatcggc tttcagttcg gcaaactgat cgtccagcga tttttgttta ccgaagctgt
    27061 ggctttctgc ttccgcttcc atctggtcaa tacgacgttc gaaagattcg aaacgagcca
    27121 ttgcttcatc cagtttgcca ctgtccagtt gacgacgcac atcgcgcgac gagtttgccg
    27181 cctgatggcg taacatcaat gcctgctggc gagcgcgtgt ttcgctcaat ttgttttcca
    27241 gctcgccgat ctctttcttc atgcgtgcca gcgtatcgtc caccagcgtc acttcatgtt
    27301 ccagtgactt aatcagatcg gtcagtttct gtttttcaat taacgcagca cgcgccaggt
    27361 cttctctatc tttcagcagt gccagttcgg ctttttcctg ccattcaact tcacgagccg
    27421 acgcttgttc aatgcggcga gtaagctgtt tcttttccgc cagcgcacgc gccgaagtag
    27481 aacgcacttc aaccagcgtg tcttccattt cctggatcat cagacgcacc agtttctgcg
    27541 gatcttccgc tttctctaac agagcgttga tgttggcatt cacgatgtcg gcaaagcgag
    27601 aaaaaatacc cataatgttg tcctcttgat ttctgcattc aggattgtcc tgctgaactg
    27661 ttaatacaat ttgcgtgcca attttttatc tttttgattt ataaagatct gattgaagaa
    27721 tcaacagcaa catgccagga tgaattagcg aattacacta acaagtggcg aatttcatca
    27781 tggcagaata caaagataat ttactcggtg aggcgaacag ctttctcgaa gtgctggaac
    27841 aggtttcgca tctcgcaccg ctggacaaac cggtgctcat catcggcgaa cgcggcaccg
    27901 gtaaagagct gatcgccagc cgcctgcatt atctctcctc ccgttggcaa gggccgttta
    27961 tttcccttaa ctgcgcggcg ttaaatgaaa atctgctgga ttccgaactg tttggtcacg
    28021 aagcgggggc gtttaccggt gcgcaaaaac gtcatccagg aagatttgaa cgtgccgacg
    28081 gcggtacgct atttcttgat gaactcgcta cagcaccgat gatggtgcag gaaaaattat
    28141 tgcgcgtgat tgagtacggt gaactggagc gcgttggcgg cagccagccg ttgcaggtga
    28201 atgtgcggtt ggtatgcgcg acgaatgccg atctcccggc gatggtcaat gaaggtactt
    28261 ttcgcgctga cctgctcgac cgactggctt ttgatgttgt acaactgccg ccactacgcg
    28321 aacgtgaaag cgacattatg ttaatggcgg aacactttgc catccagatg tgtcgggaaa
    28381 tcaagctgcc tctgttcccg ggttttaccg agcacgccag agaaacattg ctgaattatc
    28441 gctggccggg aaatattcgt gaattgaaaa acgtggtgga acgttcagtg tatcgccacg
    28501 gcaccagcga ttatccgctt gatgacatca ttattgatcc ctttaaacgg cgtccacctg
    28561 aagaagccat cgccgtttca gaaaacactt cgcttccaac actgccgctg gatttacgtg
    28621 agtttcagat gcagcaggaa aaagagttgc tgcaactcag tttgcaacaa gggaaatata
    28681 accagaagcg cgcagctgaa ttgctgggct taacctatca tcagttccgc gcgttgttga
    28741 aaaagcacca gatttagcga agttgggcaa tacaacttgc cggatgcggc gtgaacgcct
    28801 tatccggcat acataatcaa tacgtcgttt gcaggtctga taagcgcagc atatcaggca
    28861 ttattgcgtt gcccctgctt ctgcgtatta atctctcagc gcatcctgca aaattttcat
    28921 tgccgcatcg aattgcgcat ccgggatggt cagcggatac aggaaacgaa tcacgttgcc
    28981 gtatgcgcca caggtcagta gaagcagtcc ctgcgccagc gcgcgttgct ggattttctg
    29041 tgcaatcgcc gctgacggct cgcccgtttg cggatcgtta aactctgccg caatcataga
    29101 ccccagaccg cgtaccgccg cgatggccgg aacgctttct ttggcatcaa tcagcgtgtt
    29161 tgtcagacgc tggcccagtt gattcgcgcg ttagcagagt gattctttgt cgataatgtt
    29221 gagcaccgcg tgcgcggcag ccaccgccag cgggttaccg gcgtaggtgc cgccaagccc
    29281 gcccggcgcg ggtgcgtcca taatattcgc gttaccgacc acgcccgaaa gcggcatccc
    29341 gcccgcgagg cttttcgcca tcgtcattaa atccggctta tcggcgtaat gatccatggc
    29401 aaacagctta ccggtacgcg caaagccgct ttgcacttca tcagcaatca tcacaatacc
    29461 gtgctcgtcg cacaggcggc gaatggcggc aaccagctct tttggcgcaa cgttgaaacc
    29521 gccctcgccc tgcaccggtt cgaaaataat ggccgcgacc tgcttcgctt cgatgtctga
    29581 tttaaacaag cgttcgatgg cgtcgaggga gtcctgtgtt gaaatgccgt gtaaatctga
    29641 cggataaggt acgtgataca ccgaaccagg gaacgggccg aagccgattt tgtacggcgc
    29701 gacctttcca gttaacgcca tagtcatata cgtacgaccg tggaagccgc cgctaaacgc
    29761 aatcacgcca gggcgtccgg tatgggcgcg ggcgattttc accgcgtttt ccaccgcttc
    29821 cgcaccggtg gtgaagaacg cggttttggc ctgcccgctc accggggcaa gggcgttgat
    29881 tttctccgcc agggtgacgt agctttcata tggcacaatc tgatacgcgg tgtgggtaaa
    29941 ctgttgcagc tgctgctcca ccgccgcgac cagatcaggg tggcgatgtc cggtattcag
    30001 caccgcaatg cctgcggcga aatcgatgta ctcgttgccc tcaacatcct tcagcgtggc
    30061 gttttcagcc gactgggcga agaagttaca catcacgcca accccgcgcg gggtggcaga
    30121 aagacgacgc tgatggaatt cattgttgct catgtttcac accttttgac tcttcactgt
    30181 taatcggaaa ttaaacgccc agacgatcgc gcaggctgta atacgccgcg cccatcgcgg
    30241 taaacggtac gcgcagcgtg cgcccgccgg ggaacgggta atgcggcaga ttggcgaagg
    30301 catcgaaacg ttcggcgtcg ccgcgcagca gttcggcaat cagacgcccg gcgaggtggg
    30361 tacaggtgac gccgtggccg ctgtagccct gcatgtaata gatgttggta tcgaggcgac
    30421 caaactgcgg catacgcgac agggtcagca ggaagttgcc cgtccagcgg taatcaattt
    30481 tcacgccctt cagctgcggg aaggttttca gcattttcgg caccacaagg cgctcaacat
    30541 cgtccgggtc gcgagcgcca tacactacgc cgccgccgta cagcaggcgg ttatcggcgg
    30601 taagacggta gtaatcaagc agatagttac agtcttcgac gcagtagttt ttcgggataa
    30661 gggaacgggc taaatcttcc gacagccgct cggtggtgat cacctgcgtg ccgcacggca
    30721 tgctgcgttt cgccagctcc ggctccactt tatcgcccag gtacgcattc ccggcgacaa
    30781 tcacatactt cgccgtcacc tgacctgtgg ctgtacgcac tacggcgggc gtggtgtgct
    30841 gaatctgcgt cactgcggaa agctcataca cgcgcccgcc gttcaggcgg atagcgtccg
    30901 cttcgccaat ggcgaggttc aacgggtgaa tatgtccgcc gctgtgatcc aacagcgcgc
    30961 cggtgtagcg gtcgctggcc acttcacggc gaatggcgtt ggcgtccagc agttccagtt
    31021 gtttattgcc gtagcgttcc cagttctctt tctgctcttc gagtgtggcg agttgtttat
    31081 cattcatcgc cacaaacaga ccgccggggc ggtagtcgca gtcaatctga taacgtttga
    31141 tacgttcgcg gatgatctca ccaccttcga acatcatgct gccgagcata cgggcggtgt
    31201 ccatgccgta gcttttttcg atcacatcga tgtcgcggct gtaggagttc acaagctgcc
    31261 cgccgttgcg tccgctggcg ccaaagccga tgcgtgaggc ttcgagaacc actacgtcaa
    31321 agcccgcttc tgccagatgc agtgcggagg aaagcccggt atagccgccg ccgaccacgc
    31381 aaacgtcgca ggaaatcgac tcattcagcg tgtcgaatgg cgcgtattta ttcgcactgg
    31441 cggcgtagta actgctggta tgttcggtca tttcaggcct ccaggcttat ccagatggtt
    31501 ttcagttcgg tgaatttttc aagggcatgc agggatttgt cgcgaccgtt gccgctctgc
    31561 ttatagccgc caaacggcac ggtcatatcg ccgtcgttgt agttattgac gaagacggaa
    31621 ccggctttca ggcgtcggct catgcggtgc gcgcgggaga ggtcgcgcgt ccataccgcc
    31681 gcgccaaggc cgtactggct gtcgttggca agctgtagcg cctgttcttc tgatgtgaaa
    31741 cgcgtgacca ccagcaccgg accgaaaatc tcttcgcggc ttaaggacgc attcggatct
    31801 acatccacaa agatggtcgg gccgatggcg gcagccagcc cggcgttacg gccatccaac
    31861 aacagttgcc ctttgctttc gccttcccga ataaagctat ggaccgagtc ggcgtgggcg
    31921 cagtcgatta aggtgcccat ggtggttgcg ggatcaagtg gatggcccgg ctgccagttt
    31981 tgcgcctgct gttttaacag ggctaagaat tcatcggcga tgctctcttc cagcaacagg
    32041 cgcgttccgg cgatgcacac ctgaccctgg ttgtagaaga tgcctgctgc ggtggcgctt
    32101 gccgcctgtt gcaaatccgg gcagtcagcg aaaacgatgt tggcgctttt gccgcccgct
    32161 tccagccaga cgcgtttcat gttgctgtcg cccgcatctt tcagcaactg tttcccggta
    32221 cgggttgaac cggtaaaggc gatggcatcg atatcgttat gacgcgaaag cgcctgcccc
    32281 gcttcatgac caaaacccgt tactacgttc agtacgccat ccggcagacc tgcttctttc
    32341 gccagcccgg cgagacgaat cgcactgagc ggtgattttt cagacggttt tagaatcacg
    32401 ctgtttcccg ccgccagcgc cgggccgagt ttccagcaag tcagcagcag cgggaagttc
    32461 cacggcacga tggcggcaat tacgccgacc ggttcacgca cgatcatcgc cagctcatgg
    32521 ctactggtgg tcgccacttc gccatacact ttgtcgatcg cttcggcgta ccagcgaatg
    32581 gcgcgcgccg cgccgggaat atcatcacgc agactgtgac gaatcggttt gccggtgtcg
    32641 agagtttcca gcagtgccag ctcttcggcg tgggcttcca ttaaatcggc gagtttattc
    32701 agtacagctt tacgtttagc cggagaagag agtgaccagt cgccgcgttc aaatacgccg
    32761 cgtgctgcgc tcaccgcacg gtcgatatcg acgctcttgc cgcgggcaat tttggccagc
    32821 ggtgcctggg tgaccggatc aacggtttca aaggtttcat tttccgccgc agcagtatat
    32881 tcaccgttaa taaataagcg ggtttcaatg gcgagactta acgctttatc ctgccagtaa
    32941 gccagatgat gaaaattcat tatgactcct gtttcacgtc tatcagatat atgcagtttg
    33001 tgaacgtcgg ggcgcagaga tccccaggcc gtatgcccgt ttatttctgg cgacattctt
    33061 tatggattgc cgggcgcatt gcgcttaccc ggcttacaga acaaaaagat taaaacgtgg
    33121 tgggcgtatg ggcgctgata attcggcaaa tacctgccga cgtattactg aaactgtgcg
    33181 ggatgccggt attaatggca taactttgcc ccgcgacgag gtggtaatcc tgaccattaa
    33241 tcgtcagaac aatttcacct tccagtactg tgcctatttc ctcaccctga tgcttaattc
    33301 tttccccagt ggttgtgccc ggctggtacg tttcaaagat catcgccagc gtgcgattcg
    33361 ggttaccgtt atgaaccagc ttcattgaca caccctgact gcccatctca attaagtcgt
    33421 cctgattaat cacgacctgc ggctcatcag gtttttccgg ctcggaaaag aattccgaga
    33481 gtgacagacc atacaccttc agcagctttt gcagcgtact gatggcaggg ctgactttat
    33541 cttgttctat cgtactgata gcactgtgag tcagcccgga gagttcggcg gcacgacgtt
    33601 gtgaaagccc ctgctgctgg cggatttccg acaagcgttt tcctggcgcc agtccctcat
    33661 cactcatatt tgcatttcct taaactgtag tggtcagagt cgctgttttt cagcgatatg
    33721 gtgctgacaa gcggtgataa agccctcgaa caatatacgc gaaagcgcgt actcgctact
    33781 gttccattcc gggtgccact gtacgcccag cgcaaaagga tgattgatga cgctaaccgc
    33841 ctcaactaag ccatccggcg agcgagcttc aacacgcaac cgtgggctaa cgaccttcgc
    33901 cccttgccca tgcagagagt ttacccaaaa gttgctacat tcaggtaaca acgcagacag
    33961 taatcccccc tcttcaacct gaacttcatg cgacggtgca tattgctgtt ccaccggcag
    34021 ttcgggatct tcccgatgtt ccagcaattc aggctgttcg cacagcttgc gatgcaacga
    34081 accaccggtt gccaccacca gttcctgtaa accccggcag atggcgaaaa tggggatgcg
    34141 cctttcgagt gcggcattaa ttaacgccat gctcagaaga tcacgcccgg gatcggcgtc
    34201 aggctcatcg ccgttttcac catataggtg cggctgcaca ttgctgggac taccaggaag
    34261 ataaatgcca tcgagtttcg gcaaaagttg ttcaagtaat gacggttccg ccagcgcatg
    34321 tggtagcgca attggcaagc cgcctgcgtg gatgatggca ttcaggtact tttcttgcag
    34381 agtctgggtc gcatgaccct taagcctgtt cctgcacatt acgacaccga taaccggatt
    34441 gttcattata ttttccatgc tcaatctcac aaagtggact aaattatcgc cattactgcg
    34501 gtttaaatga cctttatgtt caatattttt tcaatctagc agtgggtttt catttttgca
    34561 aactcaattt aacatttgac aaacatttag tttgcataca gattcgaatg gtggtcatta
    34621 tattttacgc tttgataacg agcggaaaac aaaccaaagg cgaagaatca tggaaaccaa
    34681 tatcgttgaa gtagagaact ttgttcagca gtcagaagag aggcggggta gcgcctttac
    34741 gcaggaagtg aaacgctacc tggagcgcta cccgaatacg caatatgttg atgttttact
    34801 tactgatttg aatggttgct tccgtggaaa acgtatccct gtttcaagcc tgaagaagtt
    34861 ggagaaaggg tgttatttcc cggcctcggt gtttgcaatg gatattctgg gcaacgtagt
    34921 agaagaagcg ggtctgggtc aggaaatggg cgagccggat cgtacttgtg ttcctgttct
    34981 cggttcctta accccttctg ccgccgatcc agagtttatc ggtcagatgc tcctgaccat
    35041 ggtcgatgaa gatggcgctc cctttgacgt tgagccgcgg aacgttctta accgcctgtg
    35101 gcagcagcta cgccagcgcg gattgttccc ggttgtagcg gtagagctgg agttctattt
    35161 actggatcgt cagcgcgacg ctgaagggta tctgcaaccg ccctgcgcgc ccggcaccga
    35221 tgaccgcaat acacaaagcc aggtttactc cgttgataac ctcaaccact tcgccgatgt
    35281 gctcaatgat attgatgaac tggcgcagtt acagttaatt cccgccgacg gtgcggtcgc
    35341 cgaagcctcg ccgggtcagt ttgaaatcaa cctttaccat accgataacg tgctggaagc
    35401 ctgcgatgat gcgctggcgc taaaacgcct ggtgcgtctg atggcagaaa agcataagat
    35461 gcacgccact tttatggcga agccgtatga agagcacgcg ggcagcggaa tgcatatcca
    35521 tatcagtatg caaaataacc gtggcgagaa cgtgctttct gacgcggaag gcgaagattc
    35581 gccgctgctg aaaaagatgc tcgccgggat gattgacctg atgccgtcgt cgatggcgtt
    35641 gctggcacca aacgtgaact cgtatcgccg cttccagccg ggaatgtatg tgccgacgca
    35701 ggcgtcgtgg ggccataaca accgcaccgt cgccctgcgt attccgtgcg gcgaccgtca
    35761 taatcaccgc gtggaatatc gcgtggcggg tgccgatgcc aacccatatc tggtgatggc
    35821 agcgattttt gccggtattt tgcatggcct tgataacgag ctgccgttgc aggaagaagt
    35881 cgaaggcaac gggctggaac aggaaggctt acccttcccg attcgccaga gcgatgccct
    35941 gggtgagttt atcgagaatg atcacctgcg ccgctattta ggcgaacggt tttgccatgt
    36001 gtatcacgcc tgtaaaaatg atgagctgtt gcagtttgag cgcctcatca cagaaacaga
    36061 aattgagtgg atgttgaaaa acgcgtaata ccccttgcgg ggttgtttca tcggcctgag
    36121 cggagccagg cctgacattt gctggtaatc cagacatttc ccggatgtcg cgttatggag
    36181 cgcgggcggc aacgggcttc cgttaacgat taagactgtg acgaggaaat gagatgatgc
    36241 cgatgattaa atccccgcat ggcgagggag gctgcgtatg cgcccctcct gcgactgact
    36301 ggactccgcc ccctttgctt cccttgctaa acaggtttga tttcaggtcg acacgaccgc
    36361 aaaccttatt acgcagggga ggcagcaatt atggctatta attcaccact gaatattgct
    36421 gcgcaacccg gcaaaacccg tctgcgaaaa tcactgaaat tgtggcaggt ggtaatgatg
    36481 ggtctggcct atctcacgcc gatgaccgta tttgatactt ttggtattgt ctccggcatt
    36541 agcgacggtc acgttccagc gtcctatttg ctggcgctgg cgggagtgct gtttaccgct
    36601 atcagctacg gcaaactggt tcgccagttt ccggaggccg gttcggccta tacctacgcg
    36661 caaaagtcga ttaatccgca cgtcggattt atggtcggct ggtcatcact gctggattat
    36721 ctctttttgc caatgatcaa cgttttgttg gcgaaaatct atctctccgc cctcttcccg
    36781 gaagtgccgc cgtgggtgtg ggtggtaacc ttcgtcgcca ttttaaccgc cgcgaatctg
    36841 aagagcgtca acctggtcgc taacttcaat accctgtttg tactggtgca aatctccatc
    36901 atggtggtgt ttatcttcct ggtggttcag ggactgcata aaggagaagg cgttggcacc
    36961 gtctggtcac ttcagccgtt tatcagcgag aacgcgcacc tgatcccgat tattaccggt
    37021 gcgacgattg tctgtttctc gttcctcggt ttcgatgcgg tgaccacgct ttcggaagag
    37081 acgccggacg ccgcacgcgt gatcccgaaa gccatcttcc tgacggcggt atacggcggc
    37141 gttatcttta tcgcggcgtc gttctttatg cagctgttct ttcccgatat cagccgcttt
    37201 aaagacccgg acgccgcact gcctgaaatt gcgctgtacg tcggcggcaa gctctttcag
    37261 tcgattttcc tctgcaccac gtttgtgaac acattagcgt ctggcctggc ttcacatgcc
    37321 agcgtgtcgc gtctgctgta tgtgatgggg cgtgacaatg tgtttccgga gcgcgtgttt
    37381 ggctatgtgc acccaaaatg gcggactccg gcactcaacg tcattatggt cgggattgtc
    37441 gcgctgtcgg cgctgttctt tgatttagtc accgcgacag cactgattaa cttcggtgct
    37501 ctggtggcgt ttaccttcgt gaatctgtcg gtgtttaatc atttctggcg gcgtaaagga
    37561 atgaataaaa gctggaagga tcacttccac tatttgctga tgccgctggt tggcgcgctg
    37621 acggtgggtg tgctgtgggt taacctagag tcaacgtcac tgacactcgg tctggtatgg
    37681 gcttcgttag gcggcgcata tttgtggtat ctgatccgcc gctatcgcaa agtgccgctg
    37741 tacgaaggtg acagaacgcc ggtgagcgaa acgtaatagc cggatgcgcg gcgcattatc
    37801 ctgcctgagg gtaacgcgcc gcgccattaa ctataaaaag atgtatgacc cggatcacga
    37861 gtgctatcat ccgcccttat gcttagcgca ttaaccccag gggcaaataa tgaaccacga
    37921 tatcccgcta aaatattttg atattgccga tgagtacgcg accgaatgcg cagagcctgt
    37981 cgcagaggct gaacgcacac cgctggccca ctacttccag ttactgctca cccgtttgat
    38041 gaacaatgaa gagatcagcg aagaagccca acatgaaatg gctgctgaag cggggattaa
    38101 tcccgtacgc attgatgaga tcgcagagtt tttgaatcaa tggggtaatg agtaattatt
    38161 taattgcaga cgatcggtca gtatcgttgt ccggataaag cgttcacgcc gcatccggct
    38221 aacaatgaca cgcactgcaa cgtgctttaa tgggtttctc acgatcgtac cgctctgctc
    38281 ctcgctaagt tgttctgtga gttatagtta tagagaaatc acgggtacga atgttgaaca
    38341 gatggcgacg ccacctgttc aacgaccaac cacacggccc tcattacgac ccctgaacac
    38401 aaacggtaat ggcaaatgtc attaaatatt agtaagatag cctctttaca taaaatccct
    38461 taatattatg cgccaggtat tatcgtctct tttggtgatt gctggacttg tgagtggtca
    38521 ggcaatcgcc gcgcctgaat cccccccgca tgctgatatc cgcgacagcg gttttgtcta
    38581 ttgcgtcagc gggcaagtca acacctttaa cccatccaaa gcgagcagtg ggttaattgt
    38641 cgataccctt gccgcccagt tttatgatcg actgctggat gtcgatccct atacctatcg
    38701 cctgatgccg gaacttgccg aaagctggga agtactcgac aacggcgcga cctatcgctt
    38761 ccacctgcgt cgcgatgttc cgtttcaaaa aaccgactgg tttactccca ctcgtaaaat
    38821 gaatgccgac gatgtggtgt ttaccttcca gcgaattttt gaccgcaaca acccgtggca
    38881 taacgtcaac ggcagcaact tcccctactt cgacagcctg caatttgccg ataacgtgaa
    38941 aagcgtccgc aaactggata atcataccgt tgagttccga ctcgcccagc cggatgcttc
    39001 ttttttgtgg cacctcgcaa cccattatgc ttcggtcatg tcggcagaat atgcccggaa
    39061 gttagagaaa gaagatcgcc aggagcaact cgaccgtcaa ccggtcggca ccggtccgta
    39121 tcaattgtcg gaataccgcg ccgggcaatt tattcgccta caacgtcatg atgacttctg
    39181 gcgcggtaaa ccgttaatgc cgcaggtggt ggtggattta ggctccggcg gcaccggacg
    39241 tctgtcgaaa ctcctgactg gtgaatgcga cgttctggcc tggcctgctg ccagccagct
    39301 atccattttg cgtgacgacc cgcgcttgcg tttaacgctg cgtcccggga tgaacgtcgc
    39361 ctatctggca tttaacaccg ccaaaccgcc gctaaataat cccgctgtcc gccatgcgct
    39421 ggcactggcg attaataacc agcgcctgat gcaatccatc tattatggta cggctgaaac
    39481 ggcggcctct attttaccgc gcgcctcgtg ggcctatgac aacgaggcta aaattactga
    39541 atacgatccg gcgaaatcgc gcgaacagtt gaagtcgttg gggctggaaa atttaacgct
    39601 gaaactgtgg gtgcccacac gttcgcaggc gtggaacccc agtccactga aaactgccga
    39661 actgattcag gcggatatgg cgcaggttgg cgtaaaagtg gtgattgtac cggtagaagg
    39721 tcgctttcag gaggcgcggt tgatggatat gagccatgat ctgacgttat ccggttgggc
    39781 gacggacagt aacgacccgg acagtttctt ccgtccttta ctgagctgcg cggcaattca
    39841 ttcacagacc aacctcgccc actggtgcga tccgaaattc gacagcgtgt tgcgtaaggc
    39901 gctctcctcg cagcagctgg cagcgcgtat tgaagcctat gacgaagcgc agagtattct
    39961 ggcgcaggaa ttgcccattt tgccgctggc gtcgtcattg cgtttgcagg cctatcggta
    40021 cgatatcaaa ggtctggtac ttagcccgtt tggtaacgcc tcctttgctg gggtgtatcg
    40081 cgagaaacag gatgaggtga aaaagccatg attatcttca ccttacgccg cattttgtta
    40141 ttgattgtta ccttgttcct gctgaccttc gttggcttca gtttgagcta tttcactcca
    40201 cacgcgccgt tacaaggcgc gtcactgtgg aatgcctggg tgttctggtt taatggcctg
    40261 atccactggg attttggtgt ttccagcatt aacggtcaac caattgccga acagttaaaa
    40321 gaggtcttcc cggcgacgat ggaactgtgc atcctcgcct tcggttttgc gctgattgtt
    40381 ggcatccccg tggggatgat tgccgggatt acgcgccata agtggcagga taacttgatc
    40441 aacgccatcg ccttactggg cttttcaatc ccggtgttct ggctggcgct tctgttgacg
    40501 ctgttttgtt cactcacgct aggttggttg cccgtttcag ggcgtttcga tctgctctac
    40561 gaagtgaaac cgattaccgg ttttgcgttg attgatgcct ggctttcgga ctcaccatgg
    40621 cgggatgaaa tgatcatgag cgcaatccgc catatgatat tgcccgtgat caccctgtcg
    40681 gttgcgccaa caactgaagt gatccgcctg atgcgtatca gcactatcga agtgtatgac
    40741 cagaactatg taaaagcggc ggcgacccgc ggtttgtcgc gctttactat tttgcgtcgc
    40801 cacgttctgc ataacgcgtt accgccggtt attcctcgcc tgggcttaca gttttccacc
    40861 atgctgacgc tggcgatgat caccgaaatg gtttttagct ggccgggttt agggcgctgg
    40921 ttaattaacg ccatccgcca gcaggattac gcagccattt ccgccggagt gatggtgtgt
    40981 ggctcactgg tcattattgt taacgtgatt tctgatattt tgggtgccat ggctaaccct
    41041 ctgaaacata aggaatggta tgccttacga tagcgtatac agcgaaaagc gcccgccggg
    41101 cacgctgcgt accgcctggc gcaaatttta tagtgacgcc tctgcgatgg tcggcctgta
    41161 cggctgcgcg gggctggctg tactgtgtat ttttggcggc tggtttgcgc cttacggcat
    41221 cgaccagcaa tttctcggtt atcaattatt gccgccgtca tggtcgcgct atggcgaagt
    41281 ttctttcttc ctggggactg acgacctcgg acgcgatgtt ttaagccgct tactgagcgg
    41341 agctgcgcca accgtgggtg gcgcatttgt ggtgacgctt gccgcgacga tctgtggcct
    41401 ggtactgggc accttcgccg gggcgacaca cggcctccgc tcagcggtgc ttaaccatat
    41461 tctggatacc ttactggcga tcccttcgct gctgttggca attatcgttg tagcgtttgc
    41521 cggaccgagt ttgtcccacg ctatgtttgc tgtctggctg gcattgctgc cgcgtatggt
    41581 acgttcgatt tacagcatgg tgcatgacga actggaaaaa gagtacgtta tcgccgcccg
    41641 tctggatggc gcatcaacgc tgaatattct ctggtttgct gtgatgccaa acatcaccgc
    41701 cgggctggtc accgagatca cccgcgcact gtcgatggca attctcgata tcgccgcgct
    41761 gggctttctc gatctcggcg cacaactccc ctcgcctgaa tggggagcga tgctcggtga
    41821 tgcgctggaa ctgatttatg tcgccccgtg gactgtcatg ctgccaggtg cggcaattat
    41881 gattagcgtg ttgttggtta acctccttgg cgatggcgtt cgtcgtgcga ttattgcggg
    41941 ggtggaataa tgccattact cgatattcgt aacctgacca ttgaatttaa aaccggtgat
    42001 gagtgggtta aagccgtcga ccgggtaagt atgaccttaa ccgaaggtga aatccgcggt
    42061 cttgttggtg aatccggttc cggcaagagt ttgattgcga aagcgatttg tggggtgaat
    42121 aaagataact ggcgtgttac tgctgaccgt atgcgttttg atgatatcga tttgctgcgt
    42181 ctctccgcac gcgaacggcg caaactggtt ggccataacg tgtcgatgat tttccaggaa
    42241 ccgcagtcgt gtcttgaccc ttcagaacgt gtgggtcgcc agttgatgca aaacatccca
    42301 gcctggacct ataaaggccg ttggtggcag cgttttggct ggcgcaaacg ccgtgccatt
    42361 gaactgctgc accgcgtggg gattaaagat cacaaagatg cgatgcgcag ttttccctat
    42421 gagttgaccg aaggtgaatg tcagaaagtg atgatagcca ttgcactggc gaatcaaccg
    42481 cgtctgctga ttgctgacga accgaccaac tcaatggagc caacaaccca ggcgcaaatc
    42541 tttcgcctgc tgacgcgtct caaccaaaac agcaatacca ctattttgct tatcagccat
    42601 gacttacaaa tgcttagcca atgggcggat aaaattaacg tgctttactg cggtcaaaca
    42661 gtggaaaccg cgccgagtaa ggagttggtg acgatgccac atcatcctta tacccaggcg
    42721 ctgatccgcg cgataccaga cttcggcagc gcgatgccgc ataaaagtcg cctcaatacg
    42781 ctgcccggcg ctatcccact gctggaacag ttaccgattg gttgtcgtct ggggccacgt
    42841 tgcccgtatg cacaacgaga atgcattgtg acgccacgtt tgacgggggc gaaaaatcat
    42901 ctctatgcct gtcatttccc gctgaacatg gagaaagagt gagatgatcg aaacgctgct
    42961 tgaagtgcgt aatttaagta aaacgttccg ctaccggacc ggctggtttc gtcgtcagac
    43021 cgtagaagcg gtaaaaccct tgagctttac gctacgtgaa ggccagacac tggcgattat
    43081 tggcgagaat ggttcgggta aatccacgct ggcaaaaatg ctggcgggaa tgatagagcc
    43141 taccagcggc gagttattga ttgacgatca tccactgcat tttggcgatt attccttccg
    43201 cagtcagcgt attcgcatga tttttcagga tccttcgacc tcgttgaatc cccgtcaacg
    43261 tatctcgcaa attctggatt ttccactgcg cctgaacacc gacctggaac cggaacagcg
    43321 tcgcaaacag attattgaaa cgatgcgtat ggttgggctg ctaccggatc acgtcagtta
    43381 ctatccgcat atgttggcac ccggacaaaa acagcgtctg ggtctggcgc gcgcgctgat
    43441 attgcgccca aaagtcatta ttgccgatga agccctcgct tcactggata tgtcgatgcg
    43501 ttcgcagttg attaatctga tgctggagtt acaggaaaaa cagggcattt cgtatattta
    43561 tgttacccag catatcggaa tgatgaagca cattagcgac caggtgctgg tgatgcatca
    43621 gggcgaggtt gtcgagcgag gcagcaccgc ggatgtgctg gcatcgccgc tgcatgaact
    43681 caccaaacgg ctgattgccg ggcactttgg cgaagcattg acggcggatg cgtggcgtaa
    43741 agatcgctaa ttttctgaat cctccttcca gattatccac ctcattctga caagatttaa
    43801 tctcttgtca gaatgaggtg atggataaaa taaaatcaaa tgcgcgtgat ttaagacgca
    43861 atctcacttt gcaggaacga aagctctggc gatatcttcg cagccgacgt tttggtgatt
    43921 tcaaatttcg ccgtcaacat ccggtgggga gctacattct cgattttgct tgctgctcgg
    43981 cgcgcgtagt cgttgagctg gatggtgggc agcatgattt ggcagttgcc tatgatacca
    44041 ggcgcactag ctggcttgag tcgcagggct ggaccgtgct gcgtttctgg cataacgaga
    44101 ttgattgtaa tgaggaggcg gtgctggaga ttattctgca ggaactgaac cgccgatcac
    44161 cctcaccctg aaagggcgag ggggccgacc gagccgaata gctgttgtgg tgaaaacatg
    44221 gagacagtgc tggagaatat tcggcaaggt ctgaaccatc ccagccatcg ccataaaagg
    44281 gttaggggct gtatgagcct gtttgttgct ggggtaacaa tatttgcaca atatggtccc
    44341 ctcgcccctc tggggagagg gttagggtaa ggggaaaagc gcccaccctg ccgcagcctg
    44401 ctccggtcgg acctggcaac tatagctact cacagccagg ttgattataa taaccgttta
    44461 tctgttcgta ctgtttacta aaacgacgaa tcgcctgatt ttcaggcaca acaagcatca
    44521 acaataagga ttaaagctat gggttttctt tccggtaagc gcattctggt aaccggtgtt
    44581 gccagcaaac tatccatcgc ctacggtatc gctcaggcga tgcaccgcga aggagctgaa
    44641 ctggcattca cctaccagaa cgacaaactg aaaggccgcg tagaagaatt tgccgctcaa
    44701 ttgggttctg acatcgttct gcagtgcgat gttgcagaag atgccagcat cgacaccatg
    44761 ttcgctgaac tggggaaagt ttggccgaaa tttgacggtt tcgtacactc tattggtttt
    44821 gcacctggcg atcagctgga tggtgactat gttaacgccg ttacccgtga aggcttcaaa
    44881 attgcccacg acatcagctc ctacagcttc gttgcaatgg caaaagcttg ccgctccatg
    44941 ctgaatccgg gttctgccct gctgaccctt tcctaccttg gcgctgagcg cgctatcccg
    45001 aactacaacg ttatgggtct ggcaaaagcg tctctggaag cgaacgtgcg ctatatggcg
    45061 aacgcgatgg gtccggaagg tgtgcgtgtt aacgccatct ctgctggtcc gatccgtact
    45121 ctggcggctt ccggtatcaa agacttccgc aaaatgctgg ctcattgcga agccgttacc
    45181 ccgattcgcc gtaccgttac tattgaagat gtgggtaact ctgcggcatt cctgtgctcc
    45241 gatctctctg ccggtatctc cggtgaagta gtccacgttg acggcggttt cagcatcgct
    45301 gcaatgaacg aactcgaact gaaataatcg ttctgttagt aaagatgggc ggcgttctgc
    45361 cgcccgttat ctctgttata cctttctgat atttgttatc gccgatccgt ctttctcccc
    45421 ttcccgcctt gcgtcaggat aacgatttcc tttacgacca aggagcgccc atggaacaac
    45481 gccacatcac cggcaaaagc cactggtatc atgaaacgca atccagtact gcggagtatg
    45541 acgttctgcc tctggtcccg gaagccgcaa aggtcagcga tccctttctg ctcgacgtga
    45601 tccttgatga agaaacgctg gctcccttcc tttcatggct ggtccctgcg cgcgttcttg
    45661 cagtggaatt gttccctgac cagcttaccg tgacccgttc acagaccttc accgcttatg
    45721 aacgcttgtc tacggccctg acggttgctc aggtttgcgg cgtccagcgg ttatgtaact
    45781 actattcggc gcgacttacg ccgctccccg ggcctgattc ctccagggaa agcaatcata
    45841 ggttggcaca aatcacgcaa tatgcccgcc aactggctag ctcgccttct attatcgaca
    45901 accgatcgcg ccagcatctg aatgacgtcg gtcttactgc ctgggactgt gtgatcatta
    45961 accaaatcat tggttttatt ggctttcagg cgcggaccat tgcgacattt caggcttatc
    46021 tcgggcaccc ggtacgctgg ttacccggtc tggaaataca aaactacgcc gacgcgtcac
    46081 tgtttgctga tgaatcaata cgctggcgaa gcagctatga ggtggaaaaa ctacctgaag
    46141 agtacacaaa aagttcaaca gcagaacttt gccaactggc cgaaacactc tctctccacc
    46201 ctatttcact ttcccttctc gaaaggttgt taaacagtac acgggttaat acacagccgg
    46261 ataataagct tgcggcgttg ttatgcgcgc ggataaatgg cagtcctgct tgttttgccg
    46321 cctgtatgga ttcatcaaat gaatataaaa aaatcagccc ccttctgcgc aagggcgaaa
    46381 atgaaattaa ccaatgggct gaccgtcatt ctgttgagca cgctaccgtt caggcgatac
    46441 aatggctgac ccgagcaccc gatcgcttta gcgccgccca gttcagccct ttactcgaac
    46501 acgaaaaatc atcaacgcag attattaatc tgctggtatg gagcgggctg tgtggctgga
    46561 taaatcgctt aaaaatcgcg ttgggtgaga catattaacc ttgccgcgtc agacagattc
    46621 gcgtaaaact gtcagccgct ctaatggcca ccaaaataga caattatgtt tcaggacaac
    46681 ccgctgctag cgcagcttaa acagcaactg cattcccaga cgccacgcgc tgaaggggtg
    46741 gtaaaagcca cagaaaaagg ctttggcttc ctggaagtcg acgcgcaaaa aagttatttc
    46801 attccgccgc cgcagatgaa aaaagtcatg catggcgacc gaattatcgc ggtgatccac
    46861 agtgaaaaag aacgtgaatc cgcagagcca gaagaactgg ttgaaccgtt cctgactcgt
    46921 ttcgtgggta aagttcaggg caaaaatgac cgtctggcca tcgttcctga tcatccactc
    46981 ttaaaagacg ccattccttg ccgcgcagcc cgtggcctga accacgagtt taaagaaggc
    47041 gactgggcgg ttgccgaaat gcgccgtcat ccgctgaaag gcgatcgttc tttctatgct
    47101 gaactgacac aatacatcac ttttggtgac gaccactttg taccgtggtg ggttaccctt
    47161 gcgcgccata atctggaaaa agaagcacca gacggcgtcg ctaccgaaat gctcgatgaa
    47221 ggtctggttc gtgaagatct gaccgcgctg gattttgtca ccatcgacag tgccagcaca
    47281 gaagatatgg atgacgccct tttcgctaag gcgttgccgg atgacaaact tcagctgatt
    47341 gtggcgattg ccgatccaac cgcgtggatt gctgaaggca gcaagctgga caaagccgcg
    47401 aaaattcgcg cattcaccaa ctatctgcct ggcttcaaca tccctatgct gcctcgcgag
    47461 ctttctgacg atctctgctc actgcgcgcc aatgaagtcc gcccggtact ggcatgccgc
    47521 atgacgctct ccgctgatgg caccattgaa gataatatcg aattctttgc cgccaccatc
    47581 gaatccaaag caaagctggt gtatgaccag gtttctgact ggctggaaaa taccggtgac
    47641 tggcagcctg aaagtgaagc aattgccgaa caagtccgtt tgctagcgca aatttgccaa
    47701 cgccgcggcg agtggcgtca taaccacgca ctggtgttta aagatcgccc ggattaccgc
    47761 tttattctcg gtgaaaaagg tgaagtgctg gatatcgtcg ccgagcctcg tcgcattgcc
    47821 aaccgtatcg tcgaagaagc gatgattgcc gctaacattt gtgcggcccg cgtactgcgc
    47881 gataagctcg gttttggcat ctataacgtg catatgggct ttgatccggc gaatgccgac
    47941 gcgctggcag cgttgctgaa aacgcacggt ctgcatgtcg atgccgaaga agtgctcacg
    48001 ctggacggtt tctgcaaact gcgtcgtgaa ctggacgcgc aaccaactgg tttcctcgac
    48061 agccgcattc gtcgcttcca gtcatttgct gaaattagca ctgaacccgg tcctcacttt
    48121 ggcctcggtc tggaagcata cgccacctgg acttcgccga tccgtaaata tggcgacatg
    48181 atcaaccacc gtctgctgaa agcggttatc aaaggcgaaa ctgcgacgcg cccacaggat
    48241 gaaatcactg tccaaatggc cgagcgtcgc cgtctcaacc ggatggcaga acgtgatgtt
    48301 ggtgactggt tatacgcacg cttcctgaaa gacaaagccg ggaccgacac ccgtttcgca
    48361 gcggaaattg tcgatatcag ccgtggcggc atgcgtgttc gtttggttga taacggcgct
    48421 atcgctttta ttccggcacc tttcttacac gctgtgcgcg atgaactggt ttgcagccag
    48481 gaaaacggca ctgtacaaat taaaggtgaa acggtttaca aagtgactga cgttattgac
    48541 gtcactattg ccgaagtccg catggaaacc cgcagcatta ttgcgcgccc ggtcgcgtaa
    48601 tctcctttca cggcccattc ctcatggatg ggccgtttat ttccccgctc taccttcgtt
    48661 atatccaggc agatttttta actgactttc gtttgaaaac tggcgttttt ccaaaactgg
    48721 attacaaata aatatataaa aaaaacaatg cgtttagccg ccgttaattt catgtaagtc
    48781 atgttgttcc ttttatttaa ccgcggactc cgcagttaac cggaggatat gcatcatgaa
    48841 aaccgttagg gagtccacaa cgttgtacaa ctttctcgga tcgcacaatc catactggcg
    48901 gttgacggaa agcagcgatg ttttgcgctt ttctaccacc gaaaccacag aacctgatcg
    48961 tactttgcag ttatctgccg aacaggctgc tcgcatcagg gaaatgacgg tcatcacctc
    49021 cagcctgatg atgagtctga ccgtcgatga gagcgatctt tctgtgcatc tggtaggacg
    49081 aaaaatcaat aaacgggaat gggctggcaa cgcgtctgca tggcatgaca caccggcggt
    49141 tgctcgtgat ttatcacacg ggctttcctt tgctgagcag gtagtttctg aagcacattc
    49201 cgcaatagtc attctcgaca gccgggggaa tattcaacgc ttcaatcggt tatgtgaaga
    49261 ttacacaggg ttgaaagaac acgacgtcat tgggcaaagc gtgtttaaac tgtttatgag
    49321 ccgtcgtgaa gctgcggcat ccaggcgcaa taaccgtgta ttttttcgaa gcggcaatgc
    49381 atatgaagtc gaactgtgga taccaacatg taaaggccag cggctgtttc tgtttcgcaa
    49441 taaatttgtc cacagcggca gtggcaaaaa cgagattttt ttaatctgtt ccggcaccga
    49501 cattaccgaa gagcgccgcg ctcaggagcg actgcgtatt ctggcaaata ccgacagtat
    49561 caccggactg ccgaatcgta acgcaatgca ggatttaatc gatcacgcta ttaatcatgc
    49621 agataacaat aaagttgggg ttgtgtatct tgatttggat aatttcaaaa aggtcaacga
    49681 cgcctatggg catttgtttg gtgaccagtt attacgcgac gtgtcattgg ctattttaag
    49741 ctgtctcgaa catgaccagg tgttggcgcg tccaggtggg gatgagtttc tggtactggc
    49801 atccaacacc tcacaaagcg cgctggaagc aatggcatca cgaattttga cccgcttacg
    49861 gctccccttt cgcattggtt taattgaagt ttataccagc tgttcagtag gtattgcact
    49921 ctctcccgaa catggttcag acagcacggc tattattcgt cacgccgaca cagcaatgta
    49981 cacagcgaag gaaggcggac gaggacaatt ttgcgttttt accccagaaa tgaatcaacg
    50041 ggtatttgaa tatctctggc tggataccaa cttgcgtaaa gcactggaaa acgatcagtt
    50101 ggttattcac tatcaaccga aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact
    50161 agtacgttgg cagtcacctg aacgtgggtt gattccaccg ttggacttca tttcctacgc
    50221 cgaagagtca gggctaattg tgcctttagg ccgttgggtg attctcgatg tcgtacgcca
    50281 ggtggcaaag tggcgggata aaggcataaa cctgcgagtg gcggtaaata tttctgcacg
    50341 tcagctcgcc gatcaaacca ttttcaccgc cctgaaacag gttctccagg aactcaattt
    50401 tgaatactgc cctatagatg ttgaactgac agagagttgt ctgattgaga atgatgaact
    50461 ggcactgtct gttattcaac aatttagcca actaggtgcg caagtgcatc tggatgattt
    50521 tggtaccggc tactcttcac tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact
    50581 tgaccaggtt tttgttcgag atattcacaa acaacctgtc tcgcagtcac tggtccgggc
    50641 gatcgtcgct gtggcccagg cattgaatct tcaggtgatc gccgaaggtg tagagagtgc
    50701 aaaggaagat gcttttttaa ccaagaacgg gatcaatgag cggcaaggat ttttgtttgc
    50761 caaaccgatg cccgccgtcg ccttcgaacg ctggtataaa cgctatctga agcgcgcata
    50821 agtagatgaa tatatatact cagcgctata tcccgtcatt atatatcgta ttcctggatg
    50881 ataattagcc gttgctgtta aacacttctc ttttagcggc aatttttgca ttattcattt
    50941 tccacttttg ctataaggat gtccttgatg tctgaatttg atgcccagcg cgttgccgaa
    51001 cgtattgata ttgtgttaga tatccttgtg gcaggcgatt accactctgc gatccataat
    51061 ctcgagatac ttaaagcaga actcttgcgc caggttgctg aatctacgcc agacatcccg
    51121 aaggcaccgt gggagattta acaatcgtgc cttcaggttt tcctatttga ggatgcccgg
    51181 cgcgtcagta tttttttatt tagtattata acgttataag aattacagcg atgaattccc
    51241 gacaacaaac tattctacag atggtcattg accagggtca ggttagcgta accgatctgg
    51301 caaaagccac tggagtttct gaagttacca ttcgccagga tctcaacacc ctcgaaaaac
    51361 tgagttacct ccgccgtgca catggctttg cagtttcgct tgatagtgat gacgtcgaaa
    51421 cccgtatgat gagcaactat acgctgaagc gtgaactcgc cgagtttgcc gcgtcactgg
    51481 ttcaaccggg cgaaaccatc tttatcgaaa atggtagcag caatgccctg ctcgcccgga
    51541 ctctggggga gcagaagaaa aatgtcacta tcatcacggt cagcagctac atcgcgcatt
    51601 tgctgaaaga cgcgccttgt gaagtcattt tgctcggtgg cgtgtaccag aaaaaaagcg
    51661 aaagtatggt tggcccgttg acacgccagt gcatccagca ggtgcatttc agcaaagcat
    51721 ttattggtat tgatggctgg caacctgaaa ctggattcac cggtcgcgat atgatgcgta
    51781 ccgatgtggt caatgccgtg ctggaaaaag agtgcgaagc gatagtcctg actgacagct
    51841 cgaaatttgg tgctgtacat tcatactcca tcggtcccgt tgagcgattc aatcgcgtga
    51901 ttaccgattc gaaaatacgc gccagcgatc tgatgcatct tgagcacagt aaactcaccg
    51961 ttcacgtcgt tgacatttaa ccatttccct tctctacgga tgatttgcag tttggcaaat
    52021 catccgctct aagatgattc ctggttgata attaagacta tttacctgtt attaacacgc
    52081 ttaagatata aaattattat cagcgatata acaggaagtc attatcacct gcgtgatata
    52141 accctgcgcg cgagcagatt tcacggaata atttcaccag gcttattctt atctattata
    52201 gttatagaga gcttacttcc gtgaatcata aattcaggag agagtattat gtttgtaacg
    52261 agcaaaaaaa tgaccgcggc tgttctggca attactttgg caatgtctct gagtgcctgt
    52321 tctaactggt ctaaacggga ccgcaacacc gcaatcggcg cgggtgcagg ggcattaggc
    52381 ggtgcagtac tgaccgatgg cagtacgttg ggtacattag gtggtgcagc tgtcggtggt
    52441 gttattggtc accaggtcgg taaataatcg taaataaatg tcgattatcg actcgcaatt
    52501 ataatacgct tgattctgtt cagtacgaaa taactgaata atacgcacag taaaatattc
    52561 gaaagccacg gatatatccg tggcttttta tgttaaccgc ctgcgagttt taccttcatc
    52621 cctttcgctt caagtagtga ttttaataaa tcacgcttat cgccctggat ttcaataact
    52681 ccatctttaa ctgctccgcc gcagccgcat tttttcttca gttccgctgc aagttttgtc
    52741 agttcggcat catcgagatc gacaccggta atcaggcaaa cgcccttacc tttacgtcca
    52801 ctggtctgac gctgaatacg caccacaccg tcgcctttag ggcgtacagg ggccgctttg
    52861 ggctcatcaa tacgtccggt ttccgttgag tagaccagac ggctgttgga atcactcatc
    52921 atgcactccg ctgtaaagag gcgttgatcg ctttcagcgt ctgcgctgga tctaccgatt
    52981 gcgttaccgg gcgaccaatc accatataat caacaccagc cgacaacgcc tgttctggcg
    53041 tcataatgcg gcgctggtca ccagcttcac tcccctgcgg acgaatgccc ggcgtaacca
    53101 gtttgaactc ctgaccgaat acctgtttaa agcgcacagc ttcctgagca gaacacacca
    53161 caccatcaag gccacatttt tgcgtcagtg ccgccagacg ttctgcataa tctgcaggtg
    53221 acagtgtcat gccaagatcg accaggtcgc tggcttccat gctggtcaac actgtcacag
    53281 caatcaaaag cggtgcatct ttgccaaacg gaaccagtgc ctcacgcgct gcggtcatca
    53341 tacgcgcccc accagaggca tgaacattca ccatccacac gcctaagtca gctgcagcag
    53401 cgacagcgtg cgcagcagtg ttaggaatat cgtggaattt caggtcaaga aagatatcaa
    53461 aaccacgctg ttgaagttcg cgcacaaact gtggcccaaa caatgtaaac atctctttgc
    53521 cgaccttcag acgacaatcg cgtgggtcga tcttgtcgac aaaggacagc gcgtcatcac
    53581 gattatgata atcaagggca acaaccacag gagaattcgt aacagcgcgg gaagaagatg
    53641 aagcagttaa cgtcatgacc agaccttctt gatgatgggc accggaagtg gcgcgaaaca
    53701 ggtaaacggc gagcattcta cccgtggaaa taaaaaatta acagttgcga tttttcccct
    53761 ggcaggcttt ttctgcgccg tcgttgccgg agcataatgg aacatcaata actagtatgt
    53821 tgtaactaaa gtaggatttt tttaaaaatt acaggccatc aagaccgcga atcggtttaa
    53881 tggttgacca ggcccgacaa gacggacaat gccagtagag ggtgtatgcg gtaaaaccac
    53941 atttctggca gcgataacga ggcttactac gtaccttttc gccaaccatg tcacgcagca
    54001 ccatcaggct ctctttggca cgcccttctt ccgcttcatt taagtggtaa tccattaact
    54061 tatggaacac acgcatggtc ggatgacgct gaagctggcg cgtaatatag acctgtgcgg
    54121 cctcactacc gtcgcgcgct tcgatgatat cagcaagcat caattcagca tcggcaccgg
    54181 tgttctcttc caccgcgcgt tgcaggaatt ctgcccattc ggcagtttta cccaactgct
    54241 gatagcaggt ttgcaacatt tccagcgttt cgctgaccag ttctctgtcc tgggatatca
    54301 cgcgttgcaa actttcgacg gctttggcgt attctccttt cgccataaac acgcgtccca
    54361 tcatgatgga tacgcgggcg ctgtttttat ctgccgccgc cccttttttt agcaaggtca
    54421 tggcacgatc gagatcgtcg ctggccatat gctgcagggc taactcacag tagaaatggg
    54481 caatttcgac gcgctgttta tctttaccca gcttcaccag gcgttcggca acatcaattg
    54541 ctttctgcca ctcactggta gcctggtaga tttgtagcaa ctgttgcaga gcgccaatgc
    54601 ggaagtcagt ttcatcggtc agctgattga acatgtcttc cgcgcggtcg tataacccgg
    54661 cggccatgta atcacgccct agttgttgaa tcgccaacag acgctgttca taggtcagcg
    54721 aggcgctttc cattagggtc tgatggatgc gaatagcgcg atcaacttcg ccacgcgaac
    54781 ggaacaggtt tccgagcgta aggtgggctt caacggtgcc tgtatcctct ttaagcatat
    54841 cgagaaacag gtctaccgct ttatcctgtt gattactaag caggaagtta acccccgcta
    54901 cgtaatcacg cgacaagcgg ttggcttcat cttgcttgtt ttgttgcgca cttctgcggc
    54961 ccatatacca gccataggca gcggctacag gcaaaagcag aaacaacaac tccagcatag
    55021 aaagttattc cttcgccgct gacgagtgcg gcactacagc cacgtcagtc gcgggtgaaa
    55081 gctggttttc cagtcgcttt attttacgtt cagcgcgcgc cagggaaaca cgaactcgca
    55141 gccagaacag gccacaaatc aaccaaccga tagcaaaccc tgcagcaaac aataccgcca
    55201 gcaatgtgga aatacggtac tccccttgcg ctaacagata attaaacgtc acctgttgat
    55261 cgttctgcgc acccaacgtg accgaaatca cgaagatcgc taacaccagt aagaaaatga
    55321 gtaaatattt cacattactt cccgttatgt ggttagagcg aataaattat ggtcaactta
    55381 ccgcaatagc cctttaaagt accattttcg cgccgtctgc gaaatggcat ccacgcgcat
    55441 tgttgatgat ttaagggcta aattcggaaa atcaaccagc gttaactttc ttgttctcgt
    55501 tgcgctattt cgcgattttc ttccgcaggt ggtgttaatg gcccacaaat tcgttgcgca
    55561 agccacgttg ccaccgccac cagcgcccac gaaatcaacg tagctactac cagatcgcgt
    55621 ggccaatgca tcccgagcag caggcggctt cccatgactc ccgttgccca gaccagcaag
    55681 atagcaatgg ttaacgttcg ccgacgcggc cacagcaaac caacggccag cagtgcccaa
    55741 ctggcagcaa acatcgtgtg accggaagga aaggcaaacc ccgtctcttt ctgccagtgt
    55801 gaacgcaaat attgtgggat atttttctct tcagccaact gttctttcac taaatttccg
    55861 cgttctgctc gctttaaagt gtagaactca tcaaccggaa tatgatgtgt tttttccagc
    55921 cagataacaa aaggtcgtgg ttcctggact ttgtctttga tccaggattt aacgccttgt
    55981 cccacaagga ttgcggccgc cagaatggca aataatacaa aggcagcctt aatgcgaaaa
    56041 cgcagacacc agagaaacca gccgaataaa atcaaatgtg taatgacgcc ccagggctgg
    56101 gtgacagttt cagtaaccca aaaagccgct tttagtagcc aactttgttc tccaggttgc
    56161 caacgccagc cagaaatcca tacggctact ggcatgacaa gcaatagtgc agctcccact
    56221 gcggtacgtc tggcaatcga acgcatggcc tctccttttt gataagtccc acaatcataa
    56281 ctgaaaacgc cagttccagg aaaaattgac agatttgtgc cattccgtga acgatcgacg
    56341 cgtcgtgatt aggtgaaccc cttctcgtta tggcaaaata agccaataca gaaccagcat
    56401 tatctggaga atttcatgca gcttaaacgt gtggcagaag ccaaactgcc aaccccatgg
    56461 ggcgatttcc tgatggtggg atttgaagaa ctggcaaccg gacacgatca tgtcgcgcta
    56521 gtctatggcg atatttccgg gcataccccg gtacttgcgc gcgtccattc cgaatgtctg
    56581 accggtgacg ccctgttcag cttgcgctgc gattgtggct tccagctcga agcggcattg
    56641 acgcaaattg ccgaggaagg ccgtggtatt ttgctgtatc accgtcagga aggtcgtaac
    56701 attggtctgc tgaataaaat ccgcgcttac gcactgcagg atcaaggtta cgataccgta
    56761 gaggctaacc accagttagg cttcgccgct gatgagcgcg acttcactct ttgcgctgat
    56821 atgttcaaac tccttggcgt caatgaagtc cgcttgttaa ccaataaccc gaaaaaagtc
    56881 gaaattctga ccgaagcagg gattaatatt gttgaacgcg taccattgat tgtaggtcgt
    56941 aaccccaata acgaacatta tctcgatacc aaagccgaga aaatgggcca tttgctgaac
    57001 aaataaccct cttgcattgt gtaattagtt tgcttgccgg aagcaaaata accggcaagc
    57061 aaatacttgt tacttcaaca tattacgaat gacataatgc aaaatgccgt cgttctggta
    57121 gtaggtcaac tccgtcgcgg tgtcgatacg acaacggcag ggtacgacct cctggctacc
    57181 atccgcgcgc gtaagcgtca ccggaaccgt cgcaccgggt tgtagatttt gcagatcgcc
    57241 aatatcaatc ttctcttccc cggttagtcc taacgtttta cgcgttacgc cttgcggaaa
    57301 ttccagcggc aggatgccca tgccaattaa attcgaacgg tgaattcgtt caaacgattc
    57361 ggcaatcacc acacgaattc caagcagacg aggacctttt gccgcccagt cacggcttga
    57421 gcctgatcca tactctttcc cggcaatcac cgccagcggc gtttgttcct gcttatagcg
    57481 catcgcagca tcataaatag agactacgtc gctgtcaggt aaatgccgcg tcatcccccc
    57541 ttcaacgcca ggcaccattt cattacggat gcgaatattg gcgaaggtgc cgcgcatcat
    57601 cacttcatgg ttaccacgcc gcgaaccgta ggagttaaag tcttttcgct caacaccccg
    57661 accttgtaga tatcgacccg ctgggctgtc gggcttaata ctgcccgccg gagagatatg
    57721 gtcagtggtg actgaatccc ccagcattgc gaggatccgc gcaccgtgaa tatcttccac
    57781 tggtgctggt gttgcctgca tttcatcaaa gaaaggcgat aagcgaatat aggttgagtc
    57841 ctcctgccaa ccgtaggtat cggatcgtgt gacgttaatt tccttccact ctgctgtgcc
    57901 ttcaaaaact tctgcgtact ctttgcggaa catttctgtg gagacttgtt ctaccgcacg
    57961 ggcaatttct tgtgccgatg gccagatatc tttcagataa accgggtcgc ctttacgatc
    58021 atgaccaata ggctcagcag ccaggttgat attcatattt cccgccagcg cataggcaac
    58081 caccagcggc ggcgaggcca gccagttagt tttaaccagc ggatggatac ggccttcaaa
    58141 gttacggttg ccggacagca ccgcaccgac ggttaaatcg ccttttttga ttgccgtttc
    58201 gataggatcg ggcagcggcc cagagttacc aatacaggtg gtacaaccgt atcccacaag
    58261 gttaaacccc agttcgtcga gatacggtgt cagtttcgct tttgccagat aatcagaaac
    58321 gactttcgaa cccggtgcca gcgacgcttt gacccacggt tgccgcttga ggcccagagt
    58381 tacggctttt ttcgccagca agcctgcggc catcagcaca cttgggttag aggtgttggt
    58441 gcacgaggtt atcgcagcaa tgaccacagc gccatcaggt aactgatact gatgtccgtt
    58501 cataacataa tcgaccggct ggcgatcttt atgcgtggca ttcacttcca gttcgttact
    58561 ggcggcaaat gcttttggta catcgggcag tgcaacgcga tcctgtgggc gttttggccc
    58621 tgccaggctc gcttcaacgt cattcatatc cagttctaac gtactggtaa aaatcggttc
    58681 atcgcccggg ttacgccaca tgccctgcgc tttggcatat ttttcgacca actcaacctg
    58741 atcttcgctg cgcccgctta aacgcatgta atcgagggtt acagcatcga ttgggaagaa
    58801 gccacaggtg gcaccatatt ctggcgacat attggcaatg gtggcgcgat ccgccagcgg
    58861 tagtgaatcc agaccatcac cataaaattc gacgaatttc cccaccacgc catgtttgcg
    58921 cagcatttgg gtaacagtga gaaccaggtc tgtggcggta ataccttcac gtaatttacc
    58981 ggtaagttta aagcccacta cgtccgggat aagcatggaa accggctggc ctaacattgc
    59041 ggcttctgct tcgatcccac ccacgcccca ccccagcacg ccaaggccgt tgatcatagt
    59101 ggtgtgcgag tcagtaccaa cgagtgtatc cggataagca atccattcac cgtcctgcaa
    59161 ttcactccac actgctttgc cgagatattc gaggttaacc tgatggcaaa tgcctgtgcc
    59221 tggcggcacg acgctaaacc gactgaacgc ttgctttccc catttcagga acacataacg
    59281 ttcgtggttg cgctccattt ccaggcgtac gttttcttca aatgcctcat catcaccaaa
    59341 acgatcgacg gtcaccgagt ggtcaatgac caggtcgacc ggtgagagcg ggttaacctt
    59401 tgcagtatcg ccgccgaggc gtttaaccgc ttcgcgcatt gccgccagat caacaacggc
    59461 aggtacgccg gtaaagtcct gcatcagcac ccttgccggg cggtaggcaa tttcacggtc
    59521 agcatgggca tttttcagcc atcctgccag cgcgtggata tcctcttcgg taaccgagtt
    59581 accatcctgc cagcgcagca ggttttcgag caaaactttg agtgacttgg gcagacgggt
    59641 gatatcgccc agtgatttag cagcaagcgg caggctgtag tagtggtaag ttttatcttt
    59701 ggcctgcaac gtgtccttac tggcttctcg tagggttgac gacatagctc ctccttaatg
    59761 acagggttgc gaagccctga ttctcttcag ggtgaatatt aaagataaca caaacatcgc
    59821 gtaacgattt gataacaacc caaattgata aaagaggaga aaccttgtgg caaagcaaat
    59881 gacaaccccg ccgcagcggg gtcaaggatc agtgagtaat gatccagagg agttcggccc
    59941 agaagagcat cgacagagaa aacgcgccca tccaggacca atatttcata tttgtgttga
    60001 tcataggcta cacccttaac ggttattatt ttgtgttgag ataactcaag ctgttattgc
    60061 tgcggtgtat taaaagccct ttaattcatt aaaaatgggt aataagaatg atgataatta
    60121 ttgtttcggc tgatcatctt cagcaaacag ttctataaag gcttgctgct gcgatgttag
    60181 ttgccagtcg tctggaataa cgacggaaca ttcccgtttc gtttctgttt cattgtcact
    60241 atgctggcat aatggtgacg actccagttg ctcctgaccg accatagtta tccccacact
    60301 ttccaaatca gcagtgcgac aaccacccag aaaagtgcag aaccaagaaa aaccgccagc
    60361 caggctttac gttttaattc aggatcgcgg cggggttcct gatttccaga aggcattgct
    60421 aacctcatat aatcgccatc acttatcagc aagacgttga acgattggtg taattaatca
    60481 tacgatgaga caaatcctaa agaaacttta gtcaaaaatc cagcgaattt acgaatcgtc
    60541 tccagtgaat aattcaatct tttgacctga aatagatatt tgaaaactgg agtttgcgag
    60601 gcgggtaatt agacactact ttacaggtaa tttaccctat aaatatgagg gataaaaata
    60661 ttgtgcacgg tatagaaata agtaacaaaa gagaatatat tccggcacct tcgctacata
    60721 aaaggtgccg aaaataacgc aagaaattat ttttccggca gttttatatc tttaaacatg
    60781 acctcaattt cttcattaga gcgcaatgcg acagccgcat caacgacatc acgcgttaaa
    60841 tgcggtgcaa aacgctgaat gaaatcatac atataactgc gcaagaaagt actacggcga
    60901 aaaccaattt tggttgtact gtggctgaag atatcgtgag catcaacacg cacaaggtcg
    60961 ggatcggcga ccggatccac cgccatgctg gcaatgaccc ctacccccag ccctaaccgg
    61021 acgtaagttt taatgacgtc agcatccgtt gccgtgaaga cgatacgcgg cgttaaccct
    61081 gcgcggttaa aggcagtatc cagttctgaa cgtccggtaa agccgaaggt atatgtcacc
    61141 aacggatatt gcgccagttc ttcaatggta atggcttttt tgcctgccag cgggtgatcc
    61201 ggagtgacta caatagcccg attccagtgg tagcacggta acatcactaa atcttcatac
    61261 agatgcagcg cttctgtggc gatagcgaaa tcagcattgc ctttagagac ggcatcagca
    61321 atttgtgtcg gcgagccctg gtgcatatgc aaagaaacgc ggggataacg ctcaataaag
    61381 cctttgataa cgtttggtaa cgcgtagcgt gcctgggtat gcgtggtggc gatatacagc
    61441 gagcctttat ccggccaggt gtgctcaccg gcaaccgatt ttatggcatc gactttcgac
    61501 aggacttcgc gagcgatacg aattatttct tgtcctgctg gcgttacctg cgtcaggtgc
    61561 ttgccgcttc gggaaaaaat ttgaatgcct agctcgtctt ccagcattct gacttgttta
    61621 ctgatcccgg gttgtgatgt gtaaagtcct tccgctgttg atgagacatt caggttatga
    61681 ttgaccacct caacaatata gcgaagttgt tgtaatttca tgttaaacca tccacttaga
    61741 cgccatcaga acatcgtttt ttcgtcacac ctggcgggtg actaatacga tttaataata
    61801 aaaaaggtgc taact
//
LOCUS       DBANUU010000008        55420 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000008 DBANUU010000000
VERSION     DBANUU010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.008"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            36..1085
                     /locus_tag="ACIE68_000483"
     CDS             36..1085
                     /locus_tag="ACIE68_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFX5906852.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            1159..2016
                     /gene="nfo"
                     /locus_tag="ACIE68_000484"
     CDS             1159..2016
                     /gene="nfo"
                     /locus_tag="ACIE68_000484"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFX5906853.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            2019..3107
                     /locus_tag="ACIE68_000485"
     CDS             2019..3107
                     /locus_tag="ACIE68_000485"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFX5906854.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDET
                     VLAINDTHLLEQLSPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLAGEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3163..4413)
                     /locus_tag="ACIE68_000486"
     CDS             complement(3163..4413)
                     /locus_tag="ACIE68_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFX5906855.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWTDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(4513..5454)
                     /gene="rihB"
                     /locus_tag="ACIE68_000487"
     CDS             complement(4513..5454)
                     /gene="rihB"
                     /locus_tag="ACIE68_000487"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFX5906856.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            5584..6282
                     /locus_tag="ACIE68_000488"
     CDS             5584..6282
                     /locus_tag="ACIE68_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFX5906857.1"
                     /translation="MKEIHNNDLKLQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(6479..7729)
                     /locus_tag="ACIE68_000489"
     CDS             complement(6479..7729)
                     /locus_tag="ACIE68_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFX5906858.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(7823..8761)
                     /gene="psuG"
                     /locus_tag="ACIE68_000490"
     CDS             complement(7823..8761)
                     /gene="psuG"
                     /locus_tag="ACIE68_000490"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFX5906859.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(8749..9690)
                     /locus_tag="ACIE68_000491"
     CDS             complement(8749..9690)
                     /locus_tag="ACIE68_000491"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFX5906860.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(10114..11805)
                     /gene="fruA"
                     /locus_tag="ACIE68_000492"
     CDS             complement(10114..11805)
                     /gene="fruA"
                     /locus_tag="ACIE68_000492"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFX5906861.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(11822..12760)
                     /gene="fruK"
                     /locus_tag="ACIE68_000493"
     CDS             complement(11822..12760)
                     /gene="fruK"
                     /locus_tag="ACIE68_000493"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFX5906862.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(12760..13890)
                     /gene="fruB"
                     /locus_tag="ACIE68_000494"
     CDS             complement(12760..13890)
                     /gene="fruB"
                     /locus_tag="ACIE68_000494"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFX5906863.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGGDAEQALKAIGDAIAAGLGEGA"
     gene            14258..15439
                     /gene="setB"
                     /locus_tag="ACIE68_000495"
     CDS             14258..15439
                     /gene="setB"
                     /locus_tag="ACIE68_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFX5906864.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(15436..15690)
                     /locus_tag="ACIE68_000496"
     CDS             complement(15436..15690)
                     /locus_tag="ACIE68_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFX5906865.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            15845..16417
                     /gene="yeiP"
                     /locus_tag="ACIE68_000497"
     CDS             15845..16417
                     /gene="yeiP"
                     /locus_tag="ACIE68_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFX5906866.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            16640..18106
                     /locus_tag="ACIE68_000498"
     CDS             16640..18106
                     /locus_tag="ACIE68_000498"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFX5906867.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            18224..19210
                     /gene="yeiR"
                     /locus_tag="ACIE68_000499"
     CDS             18224..19210
                     /gene="yeiR"
                     /locus_tag="ACIE68_000499"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFX5906868.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            19249..19962
                     /gene="lpxT"
                     /locus_tag="ACIE68_000500"
     CDS             19249..19962
                     /gene="lpxT"
                     /locus_tag="ACIE68_000500"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFX5906869.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            20374..20940
                     /gene="mepS"
                     /locus_tag="ACIE68_000501"
     CDS             20374..20940
                     /gene="mepS"
                     /locus_tag="ACIE68_000501"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFX5906870.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            21121..22677
                     /locus_tag="ACIE68_000502"
     CDS             21121..22677
                     /locus_tag="ACIE68_000502"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFX5906871.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHRHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            22759..24573
                     /locus_tag="ACIE68_000503"
     CDS             22759..24573
                     /locus_tag="ACIE68_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFX5906872.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            24574..25668
                     /locus_tag="ACIE68_000504"
     CDS             24574..25668
                     /locus_tag="ACIE68_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFX5906873.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            25668..26693
                     /locus_tag="ACIE68_000505"
     CDS             25668..26693
                     /locus_tag="ACIE68_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFX5906874.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            26695..28284
                     /gene="yejF"
                     /locus_tag="ACIE68_000506"
     CDS             26695..28284
                     /gene="yejF"
                     /locus_tag="ACIE68_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFX5906875.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(28288..28632)
                     /locus_tag="ACIE68_000507"
     CDS             complement(28288..28632)
                     /locus_tag="ACIE68_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFX5906876.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(28965..30155)
                     /gene="bcr"
                     /locus_tag="ACIE68_000508"
     CDS             complement(28965..30155)
                     /gene="bcr"
                     /locus_tag="ACIE68_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFX5906877.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(30183..30878)
                     /gene="rsuA"
                     /locus_tag="ACIE68_000509"
     CDS             complement(30183..30878)
                     /gene="rsuA"
                     /locus_tag="ACIE68_000509"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFX5906878.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAVVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            31028..32788
                     /locus_tag="ACIE68_000510"
     CDS             31028..32788
                     /locus_tag="ACIE68_000510"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFX5906879.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            32913..33197
                     /gene="rplY"
                     /locus_tag="ACIE68_000511"
     CDS             32913..33197
                     /gene="rplY"
                     /locus_tag="ACIE68_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFX5906880.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(33336..34343)
                     /gene="yejK"
                     /locus_tag="ACIE68_000512"
     CDS             complement(33336..34343)
                     /gene="yejK"
                     /locus_tag="ACIE68_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFX5906881.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAQAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            34525..34752
                     /locus_tag="ACIE68_000513"
     CDS             34525..34752
                     /locus_tag="ACIE68_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFX5906882.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            34772..36532
                     /gene="yejM"
                     /locus_tag="ACIE68_000514"
     CDS             34772..36532
                     /gene="yejM"
                     /locus_tag="ACIE68_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFX5906883.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            36607..36683
                     /locus_tag="ACIE68_000515"
     tRNA            36607..36683
                     /locus_tag="ACIE68_000515"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36641..36643,aa:Pro,seq:ggg)
     gene            complement(36784..39153)
                     /locus_tag="ACIE68_000516"
     CDS             complement(36784..39153)
                     /locus_tag="ACIE68_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFX5906884.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGG
                     LAIAVDQKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYELDDGSGLIVMEDTQAI
                     DTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSSKAISENMVINNGRANV
                     WAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGASFRTHGAVDTSKADVSL
                     ENSVWTIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRSSVTASAENFITLTTNT
                     LSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASPAAGDSLTLVTTGGGDA
                     AFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTTDVLNMAAAQPLVFDAE
                     LDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQTLTGLTLGIDSRFSREE
                     SSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNGAYVDGVVKVDRFANTI
                     HGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAFTGFTTDGQDYTLSNGM
                     RADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEYADSNQVKVNDDGKFNN
                     DVAGARGVYQAGIRSSFTPTLSGHLSVSYGNGAGGESPWNTQAGVVWTF"
     gene            39473..40120
                     /gene="narP"
                     /locus_tag="ACIE68_000517"
     CDS             39473..40120
                     /gene="narP"
                     /locus_tag="ACIE68_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFX5906885.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(40155..41207)
                     /gene="ccmH"
                     /locus_tag="ACIE68_000518"
     CDS             complement(40155..41207)
                     /gene="ccmH"
                     /locus_tag="ACIE68_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFX5906886.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(41204..41761)
                     /gene="dsbE"
                     /locus_tag="ACIE68_000519"
     CDS             complement(41204..41761)
                     /gene="dsbE"
                     /locus_tag="ACIE68_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFX5906887.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(41758..43701)
                     /gene="ccmF"
                     /locus_tag="ACIE68_000520"
     CDS             complement(41758..43701)
                     /gene="ccmF"
                     /locus_tag="ACIE68_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFX5906888.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(43698..44177)
                     /gene="ccmE"
                     /locus_tag="ACIE68_000521"
     CDS             complement(43698..44177)
                     /gene="ccmE"
                     /locus_tag="ACIE68_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFX5906889.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(44174..44383)
                     /gene="ccmD"
                     /locus_tag="ACIE68_000522"
     CDS             complement(44174..44383)
                     /gene="ccmD"
                     /locus_tag="ACIE68_000522"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFX5906890.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(44380..45117)
                     /gene="ccmC"
                     /locus_tag="ACIE68_000523"
     CDS             complement(44380..45117)
                     /gene="ccmC"
                     /locus_tag="ACIE68_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFX5906891.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(45159..45821)
                     /gene="ccmB"
                     /locus_tag="ACIE68_000524"
     CDS             complement(45159..45821)
                     /gene="ccmB"
                     /locus_tag="ACIE68_000524"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFX5906892.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(45818..46441)
                     /gene="ccmA"
                     /locus_tag="ACIE68_000525"
     CDS             complement(45818..46441)
                     /gene="ccmA"
                     /locus_tag="ACIE68_000525"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFX5906893.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(46454..47056)
                     /gene="napC"
                     /locus_tag="ACIE68_000526"
     CDS             complement(46454..47056)
                     /gene="napC"
                     /locus_tag="ACIE68_000526"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFX5906894.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(47066..47515)
                     /gene="napB"
                     /locus_tag="ACIE68_000527"
     CDS             complement(47066..47515)
                     /gene="napB"
                     /locus_tag="ACIE68_000527"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFX5906895.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(47512..48375)
                     /gene="napH"
                     /locus_tag="ACIE68_000528"
     CDS             complement(47512..48375)
                     /gene="napH"
                     /locus_tag="ACIE68_000528"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFX5906896.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(48362..49057)
                     /gene="napG"
                     /locus_tag="ACIE68_000529"
     CDS             complement(48362..49057)
                     /gene="napG"
                     /locus_tag="ACIE68_000529"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFX5906897.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(49064..51550)
                     /gene="napA"
                     /locus_tag="ACIE68_000530"
     CDS             complement(49064..51550)
                     /gene="napA"
                     /locus_tag="ACIE68_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFX5906898.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(51547..51810)
                     /gene="napD"
                     /locus_tag="ACIE68_000531"
     CDS             complement(51547..51810)
                     /gene="napD"
                     /locus_tag="ACIE68_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFX5906899.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(51800..52294)
                     /gene="napF"
                     /locus_tag="ACIE68_000532"
     CDS             complement(51800..52294)
                     /gene="napF"
                     /locus_tag="ACIE68_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFX5906900.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            52403..52567
                     /gene="yojO"
                     /locus_tag="ACIE68_000533"
     CDS             52403..52567
                     /gene="yojO"
                     /locus_tag="ACIE68_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFX5906901.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            52681..53190
                     /gene="eco"
                     /locus_tag="ACIE68_000534"
     CDS             52681..53190
                     /gene="eco"
                     /locus_tag="ACIE68_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFX5906902.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(53681..55327)
                     /gene="mqo"
                     /locus_tag="ACIE68_000535"
     CDS             complement(53681..55327)
                     /gene="mqo"
                     /locus_tag="ACIE68_000535"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFX5906903.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPKPVQKEVADIAL"
BASE COUNT        13470 a        15003 c        13693 g        13254 t
ORIGIN      
        1 agtcgtaacc ttatgcccgg ttaaaggaga gggttatgac gaatatcacc ttacagaaac
       61 aacatcgtac actgtggcat tttattccgg ggttagccct gagtgcagtt atcaccgggg
      121 tcgccctgtg gggtggttcc attcccgcgg ttgcgggtgc cgggtttagt gccctcaccc
      181 tcgcaatctt gttggggatg gttttaggca acaccattta cccgcacatc tggaaaagct
      241 gtgacggtgg tgtgctgttt gccaagcaat atttattacg tctgggtatc attctttatg
      301 gcttccgtct gacgttctcg caaattgccg atgtcggtat cagtgggatc atcattgacg
      361 tgctgacgct gtccagtacc ttcctgctgg cttgcttcct ggggcagaaa gtgtttggtc
      421 tggataagca caccagctgg ttgatcggtg ccggtagcag tatctgtggt gctgccgcgg
      481 tactggcgac tgagccggta gtgaaagcgg aagccagtaa agtaaccgtg gctgttgcaa
      541 ccgttgttat cttcgggacc gtcgcgattt tcctctaccc ggcgatatat ccgctgatgt
      601 ctcaatggtt tagtccggaa actttcggta tctacattgg ttctactgtg cacgaagtgg
      661 cgcaggtggt ggcggcaggt catgccatca gcccggatgc ggaaaacgca gcagttattt
      721 ccaaaatgct gcgcgtgatg atgctggctc cgttcctcat cctgctggcg gcgcgtgtta
      781 aacagctgtc tggggcgaac agcggcgaga aaagcaaaat cactattccg tggtttgcca
      841 tcttgttcat cgtagttgcc atctttaact cgttccacct gttaccgcag agcgtggtga
      901 acatgctggt aacgctggat accttcctgc tggcaatggc gatggcggcg ctgggtctga
      961 ccactcacgt cagcgcgctg aaaaaagccg gggcgaaacc tctgctgatg gcactggtac
     1021 tgtttgcctg gctgattgtt ggtggtggtg cgattaacta tgtgattcaa agcgtcatcg
     1081 cataaaccac tacatcttgc tcctgttaac ccgctatcat taccgttttc ctccagcggg
     1141 tttaacagga gtcctcgcat gaaatacatt ggagcgcacg ttagtgctgc tggcggtctg
     1201 gcaaatgccg caattcgcgc cgccgaaatc gacgcaaccg cgtttgcctt gttcaccaaa
     1261 aaccaacgtc agtggcgtgc cgcaccgctc acgacgcaaa ccatcgatga attcaaagcc
     1321 gcctgtgaaa aatatcacta cacatcggcg caaattcttc cccacgacag ttatctgatt
     1381 aacctcggac atccggtcac tgaagctctg gaaaaatcgc gcgatgcctt tatagatgaa
     1441 atgcagcgtt gcgaacaact agggctttct ttgctcaact tccaccctgg cagccatctg
     1501 atgcagattt cagaagagga ttgccttgcg cgtattgccg aatccatcaa cattgcgctg
     1561 gataaaactc aaggtgtgac cgcggtgatt gaaaacaccg ccggtcaggg cagtaactta
     1621 gggtttaaat tcgaacatct cgcggcgatt atcgacggcg tggaagataa atcccgtgtc
     1681 ggcgtctgca ttgatacctg ccatgctttc gctgccgggt atgatttgcg tactccagcc
     1741 gaatgcgaga aaacgttcgc tgattttgcc cgtattgtcg gctttaagta tctgcgcggg
     1801 atgcacctta acgatgcgaa aagcaccttt ggcagccgcg ttgaccgcca tcatagcctc
     1861 ggtgaaggca atatcggtca tgatgcgttc cgctggatca tgcaggacga ccgtttcgac
     1921 ggcattccgc tgatccttga aaccatcaac ccggatatct gggcagaaga gatcgcctgg
     1981 ctgaaagcgc aacaaactga aaaagcggta gcctgaagat gaataaccgg gaaaaggaga
     2041 tccttgcaat tttacggcgt aacccgctga ttcagcagaa cgaaattgcg gacatgctgc
     2101 aaatcagtcg ttcgcgcgtt gcggcgcata ttatggattt aatgcgcaaa gggcggatta
     2161 aaggcaaagg ttacattctc accgagcagg aatactgcgt agtggtgggg acaatcaata
     2221 tggatattcg cgggatggcg gatatccatt acccgcaagc ggcttctcat cccggtacaa
     2281 ttcattgctc ggcaggcggc gttggacgca atatcgccca caatctggcg ctgttaggcc
     2341 gcgacgtcca tttgctttca gtgattggcg atgactttta tggcgaaatg ctcctggaag
     2401 aaacgcgccg cgccggcgtg aatgtctccg gctgcgttcg tttacatggt caaagcacat
     2461 cgacgtatct ggcaattgcc aatcgagacg atgaaaccgt gctggcgatt aacgataccc
     2521 atctgctgga acagttatcg ccgcaattat tgaacgggtc gcgcgattta cttcgtcatg
     2581 cgggcgtggt actggctgat tgcaacctga cagccgaggc gctggaatgg gtctttaccc
     2641 tcgctggtga gatcccggtg tttgtcgata ccgtttcaga attcaaagcg ggcaaaatca
     2701 aacactggct ggcgcatatt cacaccctga aacccacttt accggagctg gaaattttat
     2761 ggggacaggc gatcaccagc gatgctgacc gtaatgctgc agtgaatgcg ttgcatcagc
     2821 aaggtgttca gcaactgttt gtttatttgc ccgatgagtc agtttattgc agcgaaaagg
     2881 atggagaaca atttttgctg accgcaccag cgcatacgac agtagacagt tttggtgctg
     2941 acgatggttt tatggcgggc ctggtatata gctttctgga aggaaacaat ttccgtgaca
     3001 gcgcccgttt tgcgatggcc tgcgcggcaa tttcacgcgc cagcggcagc ttaaacaacc
     3061 ctaccctgtc tgccgataac gcgctttcat tagtgccaat ggtgtaacaa tgttgccgga
     3121 ttcgacgctt gaccgcatct tatccggctc ataaatcgca cactacgcta aaccaataaa
     3181 gaacccggca atggtggcac tcatcaggtt agaaagtgtc gccgccgcca gcgcgcgtaa
     3241 accaagctgg gcgatttccg gcgcacggtg tggcgcaacc gcagaaaacg cccccaccac
     3301 caccccgata gaaccaaagt tagcgaaacc gcacaacgcg aaggaaataa tcgccacggt
     3361 tttagcatcc agagtgccag ccgtttgcag atagggtgag aaattgagat aagcgacaaa
     3421 ttcatttatt gccagtttct gtccaatcaa actcccggca agattcgcat cagtccagtc
     3481 aacacccatt acccacgcca gtggggccag caggtaacct aaaatggact ccagcgaggc
     3541 atgttcaaaa ccaaaccagc cgccaacgcc gccgataata ccgttaatta acgcaatgat
     3601 ggcaacaaat gccatcacca ctgttgccac acccgcagcg attttcagcc cggtcatcgc
     3661 ccctgtcgcg gcggcttcaa taatgctttt tggcggtgtt tcggtgaaag agaggttatt
     3721 aaaagaaacc tgcgaagatt ccgtagccgg gcttaacagg cgggcaaaca agatcccgcc
     3781 tgggatcgcc attaacgatg ccgccagcaa atattccaca ggtacgccca gtgcggcgta
     3841 accaatcatt gtcgaaccgg caattgaggc catgccacta caaatcgctg taaataattc
     3901 attgcgattc agccgatcga taaagggttt gacgattgcc ggaatttcgt tttgcccgag
     3961 gaaaatggta gtgaccgcga cgaatgactc gatcttgctg atatttaatg ctttctggaa
     4021 gataccgccg agaatgcgaa ttaagatccc catcacaccg atgtaataga gaatactcac
     4081 cagcgcggtg acgaagataa ttgccggtaa tactctgaaa ccaaagataa atcctgcgcc
     4141 atcaaacagc gtgtccattt tcggcccgac cagcgaaccg aagataaatg cgctacccgc
     4201 gtcgctgtac gccatcactt tatgcacgcc aaaagcgact ttttcagcga cccaacgccc
     4261 tggcggtaac caaagcataa tgccgccaat cacaacctgt aacactaacg ccgcgccaac
     4321 ggtacgcagg ctgatcttct tcttgtttac tgacagcaaa aatgcaattg ccagcaatac
     4381 caccattccc agaacacttc tcatgacatc catagtgatt atctcttcat gtcatattgg
     4441 gcgacggctt tctgcccctc agaaatagcc aggcctgcat cttgcgatac cggcccggct
     4501 tgttatcaac acttaatggg ttttgatgta gccgcgcacg cactcttcga ctaagcccca
     4561 gaaccagtcg gtatcaatag tgatgccgac tttggtatta gcgggcttgc caagaacgcc
     4621 cagctcgtcg cagacggtac gcccataaca agggccactg ttgacgtcca cttcgacgta
     4681 catctcctgg gttttaatgc catcagggtt aatcagataa ccgatgcagg tggcgtcgtg
     4741 caccgggccg ccagccaggc cgtagttttc gaactgcgtt ttgagagtga agttcatgat
     4801 gtcgctgaac agctctccgg cagggccgcc tgccctttcc atccgagcaa ttacgtccgg
     4861 ggtgcaaacg gtctggttgg taagatcgag gcccatcatc actaatggaa cgccagaggt
     4921 gaacaccacg cgtgcggctt ccgggtcagc aaagatgttg aactcggcag atggcgtgaa
     4981 gttgcctgta ccgtaagcgc cgcccattag cacaatttca cggattttgg gcaggatcgc
     5041 gggttgcata cgcattgcca ccgcgatatt tgaaagcgga ccaaccggca ccagagtgat
     5101 atcgccatcg cttgccatca gggtatcaat gatgtatttc accgcgtggg tactttctgc
     5161 ctggcgggtc agcggctcga ataccgggcc atccagtccg gtttcaccgt gaatattatc
     5221 ggcaacgatt tgttgacgca taatgggctg cggcatcccc gcataaaccg gaacattaat
     5281 ctccagtttc tggcaaacat tcaggccatt aattaatgtt ttatcaagcg tctgattacc
     5341 cgctacaata gtgatgccta ataaatctat tgccggatgt ttcgctgcca tcatcattgc
     5401 aatagcatca tcatgacccg gatcacaatc cagaataatt tttctctttt ccattgttta
     5461 tttcctctgt ttccagttgc gttatttttt ctacagcaaa gaaaattcga ccgggccgat
     5521 gattgaatct taacaacagc gtacgtatgc taaatatgag aaatctcata gcggataaac
     5581 atcgtgaaag aaatccacaa taatgatctt aagctgcaat tgatgagtga atccgcgttt
     5641 aaggattgct ttttaacgga tgtttcagcc gatacgcggc tgtttcattt tttagcgcgt
     5701 gactacattg tgcaggaagg gcaacagccg tcctggctgt tttacctgac gcgaggccgc
     5761 gccaggcttt acgccacgct agctaatggt cgcgtgtcgc tgatcgattt ctttgccgcc
     5821 ccctgtttta ttggcgagat tgagttaatc gataaagacc atgaaccacg tgcagtacag
     5881 gcgattgaag agtgttggtg ccttgcgctc cctatgaaac attaccgtcc gctgttatta
     5941 aacgacacgc tatttttacg aaaactttgc gtcaccttaa gtcataaaaa ttatcgtaat
     6001 attgtttctt taactcagaa tcaatcattt ccgttagtga atcgcctggc agcatttata
     6061 ttactctcgc aagaaggtga tctttatcac gaaaagcata cgcaagcggc agagtattta
     6121 ggcgtttctt atcgacatct tttatatgtt ctcgcgcaat tcattcacga cggtttatta
     6181 attaaaagca agaaaggata tctaattaaa aacagaaagc agttgtcagg actggcgctg
     6241 gagatggacc cggagaataa attctccggg atgatgcagt aaaaaaataa tctggaatat
     6301 actctaaata attcgagttg caggaaggcg gcaagcgagt gaatccccag gagcttacat
     6361 aagtaagtga ctggggtgag cgaacgcagc cgcagcacat gcaacttgaa gtatgacgag
     6421 tatagtacgc gattgccgga tgcaacgctt aataagcgag tacatccggc aatgaaactc
     6481 aagctaaacc aataaagaac ccggcaatgg tcgcgctcat caggttggaa agcgtcgccg
     6541 ccgccagcgc ccgtaaacca agctgggcga tttccggcgc gcgttttggc gaaatagccg
     6601 aaaacgctcc aacgacaaca ccgatagaac caaagtttgc aaacccacag agggcgaacg
     6661 agataatggc aatggtcttg acatccagcg tgccgctcgt ttgcagatag ggcgagaaat
     6721 tgagataagc aacaaactca ttaatcgcca gtttctgccc aatcaaactt ccggcaagat
     6781 ttgcatcact ccagtccacc cccataatcc atgccaacgg ggccagcaca tagccgaaaa
     6841 tgctttccag agagacattg gcaaaaccaa accagccgcc aactccacca ataatgccgt
     6901 tgatcagcgc aatgatcgcc acaaatgcca ttaccaccgt cgccacaccg gcggcgattt
     6961 tcagcccggt cattgcaccg ttcgccgcag cttcaataat gctttttggc ggtgtttcgc
     7021 taaacgacag attttcaaac gtaacttgcg aaggttccgt cgccggacta agaatacgtg
     7081 caaacaggat accgccagga atagccatca gcgaagccgc taacaggtag tcaattggta
     7141 cgcccattcc ggcataacca atcatcatcg aaccagcaat ggacgccatc ccgctacaaa
     7201 ttgcggtaaa caactcgttg cgattcatgc gatcgataaa cggtttaacg atcgccggga
     7261 tctcattttg cccgaggaaa atagttgtga ctgcgacaaa agattcgatt ttgctgatgt
     7321 taagagcttt ctggaaaatg ctgccaagga tgcgaatcag cagccccatc acgccaatgt
     7381 agtacagcag actgatgagc gcagtaacga aaataatcgc cggaagtacg cgaaaggcga
     7441 agataaaacc cgcaccgtca aacaggacat ccattttcgg cccaaccagc gaaccaaaaa
     7501 tgaaggcgct accggcatca ctgtaagaca tcactttatg aacgcctaat gccgcctgtt
     7561 ctactgccca ctttcccggt gggaagtaga gcatgatgcc accaatagcg atttgcagca
     7621 gcagtgcggc tccaaccgtg cgcaaactga tgctcttttt attcactgac aacagaaatg
     7681 ctattgccag taacaccacc atccccacaa cacttctcat tatatccata atgattttcc
     7741 cttcatgcca gtaaacccgg cgtcagcgcc aggttttggt atgcttgatg agtacgggcg
     7801 acggctttct gcccgtcaga aattaacccg cgagacgctg gtattctttg gcaatttcac
     7861 tcgccagaat ggcgttgttg aacaccagct ggatattgga tttcaggctg tcaccgccgg
     7921 tcagctcagc aacgcgcgcc agcaggaacg gtgtactttc tttaccaata acgccctgcg
     7981 cttcagcttc agctaccgcc tgatcgatcg ccgcattgat agtgtgttcc ggcatcgcaa
     8041 actgttccgg gatcgggttc gctaccacga ggccaccgtt cagcccgctt tgccacttca
     8101 ccgccattgc acgggcaatt tcactggcgc tgtcgagacg aatgctgacg tcaaacgggc
     8161 tggtacggca gaaaaacgca ggcagcgctt ttgtctgata gccaattaac ggcacaccga
     8221 aggtttctaa atattctgtt gttaatccta aatcgagaat agatttcgcc ccggcacaaa
     8281 caacggtgac attagtattt gccagttctt gcaaatcggc agaaatatcg aaggtatgtt
     8341 ccgccccgcg atgcacacca ccaattcccc cggtggcaaa tactttaatt ccggcaagcg
     8401 ccgcaataat catcgttgaa gccacagtgg ttgcgccatt ttttccggcg gcaacaacaa
     8461 aaggtaaatc gcgacgacta actttggtca cgttatgccc ttcacgaccc agtaattcaa
     8521 tttcttcttt gcttaaaccc actttcatca cgccgccaat aatggcgata gtggcaggta
     8581 ctgcgccctg tttacgaata gtttcttcta cttcaattgc ggtctgggca ttttgtggga
     8641 acggcatccc gtgagaaata atggtcgatt ccagcgccac aaccggtttt ttgtttttta
     8701 aagcgtcctg cacttccggg gaaatttgta ataattcagg ggaaattttt aattcagaca
     8761 ttctgcgttc tccactaacg atataacgtt ggcaatcgat agatcggggt tattggtgta
     8821 ttcacaggag agcgccattg acgaacatcc ctgtgcgaaa cgaacagatt cggcaaacgg
     8881 cattccgtct acccaacacg aagcaagtcc cgccatcatg gcatcgcccg ctccggtaac
     8941 attaataaca ttggttttga tcggcgcaga ccagccattt tcaccgctga tatcgctgta
     9001 ataaacgccg tcgccgccca tgctcaatac cagtcggttc aggccatgtt gatggaacca
     9061 ggcagcaact tttgccacat cgtcacgccc tgacagcgca atcccactca gggtttccgc
     9121 ttcaaggcgg tttggcttga gagtgtggat ctgatttaga cggtcgcgca ctttgacaca
     9181 tttccacgct gaaaccggat cgacaaatac gggtacgttg gcggcattat ccagaatcca
     9241 tgccagtgcc tcttcactga tattacagtc tgcgacaatg acctttgccc tctgaataaa
     9301 ttcacggtgc tgtgcgagat attcagctgt aatagcgttg ctaatattca tgtcatttat
     9361 agcaaccagc atttcaccgg tattatcgag taatgataaa tagcttgacg tattttctcc
     9421 tggcacaatc aggcatttat cgacataaac gccagattga ttggtttgcg ttagcagcga
     9481 ttgaccataa aagtcactgc ctacggcgct cagtagccag gctttgttcc ccagcaacgc
     9541 caggttttgt gcaatattgc gcccgactcc accaggcgta aattttattt tacctggatt
     9601 tgaatccgca taatttaatg attcatgtga atatccggcg acatcaatat tcgccgagcc
     9661 tataattacg acataatcct tttcgcgcat agcacgtcct ctggtaaaaa attaccaacc
     9721 ctgtcaatcc acaagaatat agatactgca aagacggttt aaacatgtgt tcacatttaa
     9781 acatgtgctc atagtatgca tgttttacgt aaagtaaatt accgccagag aggctaaatg
     9841 ggcagatttg agcaggctca caaaatgcga ttaaatgcct gaattacaaa agcgaggaat
     9901 ttgtgacggg atgcacagaa caggaacaat aaaaaagggc agaaaataat ctgcccttga
     9961 ggaaataacc ttagtgtttg atgaacgttt caagtttcta ctgaaagata aaaccattta
    10021 aattcataaa attacattca caagctggtc attccctttg cgctcatcca atgatacagg
    10081 gcaggaaatt tctgccctgt aacacacctt ttattacgct gctttcgcta ctgcgtccac
    10141 ttccggacgt ttcaggaagg cataggccaa acccgccacc agcgtaccgg caataattgc
    10201 taccaggtaa cccagtaccg gcgtaatagc gccagggatc agcagaacaa acagaccacc
    10261 gtgtggtgcc atcagtttcg caccaatcgc cattgagatt gcgccagtca gcgcaccacc
    10321 cacgatacag cacggtagca cacgcatcgg atcacgagca gcaaacggaa ttgcaccttc
    10381 cgaaatgaag cacagtccca ataccagagc ggctttgcca ccttcctgct gcgctttgtc
    10441 gaatttgcga cgcgccacca ttgttgccag acccattgcc agcggtggca ccatacctgc
    10501 cgccataatc gccgccatcg ggccataggt ttgagtactc agcagaccca caccgaatgc
    10561 gtacgctgct ttgtttaccg gaccgcccat gtcagtacac atcatgccac cgaggatcgc
    10621 ccccagcaga accgcattcg cagtccccat ggtctgcagc cagtgagtca gcccttcgag
    10681 aatgccagca accggtttac cgatcaggta gatcatcgcc agaccgacca ccagactgga
    10741 aattagcgga atgatcagga ttggtttcag cgcctccata ctctgtggca gtttcagttg
    10801 cgtactgatt aacttcgcaa tgtaaccagc caggaagccc gcaataatac cgccaatgaa
    10861 gccagaaccg gtgctgacag ccagcatacc gccaatcagc cccggcgtga ggcccggacg
    10921 atcggcaatg gagaaggcaa tataacctgc cagtaccggc accatcagcg caaaggctga
    10981 accaccgcca atctgcatca gcgccgctgc caacgtaccc ggctctttaa acgcttcgat
    11041 accaaaagca aaagaaagcg cgatacacag accacctgca acaaccatcg gcagcatgta
    11101 agagacgccc gtcagcaagt gacggtaagc gcctgcactc tctttcttac cttcagtggt
    11161 cgctgtttga gctttgcccg ctggttcata cggcgttgct tcagcaaccg ctttatccag
    11221 ttcctgcgcg gttttcttca gcgccagacc ggtagaggta cgatacatcg gtttaccagc
    11281 aaatttcgcc agatccactt cgatatctgc cgccacaatc accagatccg ctgcggctac
    11341 ttcctcggga gtgattgcat tacccgcgcc aacagaacca cgggtttcaa ctttcaccca
    11401 ccagccacgt tttttcgctt cggtttcaat ggcttcagcc gccataaagg tgtgtgctac
    11461 gccagtcggg caagcagtca ccgcaactac gcgtttcgga ccgctggcgg caaccggtgc
    11521 tgtcgcagtg accggcgcag tgtaaggttt cgcatggcct ttggcttcac tcaggaacag
    11581 ctcagggtgc gcaactgccc gggaaatatc acccagccag acatttttac cgttcagcgc
    11641 gctgtcgttc gggatggaat caccgagaac aatcgccatt tcagcgtcgt tcggattgtc
    11701 gatgatttcc agttttgctt ttcgcgccgc cgcgcccagc agggtcttcg ccatataggc
    11761 gcgtgcctga ccgagattag cgtcaataat cagcagcgtt ttcattatgc ctctcctgct
    11821 gtcagttaaa aggttgtaag tcgacgcgcg ccatcattgc ggccaactgc ggacgatcgg
    11881 taatacccac attgctttga cttaccgcca gggctgcaac agctgtcgcc agacgcagtg
    11941 tgtgttcact ggattcacgc atcagcaagc cataaatcag gccaccaacc atagaatccc
    12001 ctgcgccaac ggtgcttacg acatcgactg acggtggttt ggcgatccat tcgccggagg
    12061 cattaaccca aagcgcgcct tcggcaccca gtgaaataac aacatgcgcg atgccttgtt
    12121 cacgtagcgc atgtgcagct tcaatcacat ctttcatttc aggcagttta cggcctgccc
    12181 agatttccag ctcgcggcgg ttaggtttca ccagccacgg tgccgctttc aaacctgcta
    12241 ctaacgcttc acggctacta tcaaagataa tgcaaggaca ctgactacgc aggcgagtca
    12301 tccagtcggt gaacgcttcc gggctgacgc ctgacggtaa gcttccgctg acacagacca
    12361 tatcgaactg accgagccag ctcagagaat cagtcacaaa gcgttcccag tcggcggggg
    12421 tgacttcaaa acccgagaag ttgaagtcgg tcacttcacc gtctttttcc gtcagcttaa
    12481 cgttaattcg ggtgcgcccc tgtacaacct ggaaacggtt ggcaatgccc agctcgctga
    12541 acagttgctg aaaaccatcc tgattgtctt taccgaggaa gccgccaacg gtgacatcaa
    12601 ttcccaggtc ttttaatact ttggccacgt tgatgccttt acccgccgca tgcagaccgg
    12661 tggttttcac caggttcact tcgccgcgtt caatttccgg gcagaaacca acaaggtcat
    12721 aagccggatt aagggtgata gtagcaacac gtctgctcat tatgcgccct ccccaagacc
    12781 agcagcgata gcgtcgccaa tggctttcag cgcctgttca gcatctccac cctgggcggt
    12841 aaagcgtagg cgatgacctt tcttaacgcc aagtgccaca actttcatca gactacgtcc
    12901 gtttgccggt ttgccggtgc catcaaggtt tgtcacggta atatcactgt taaattgttt
    12961 aatggtattg accagcatgg tacctggacg agcatgcagg ccgtgttcat tgcgcaccac
    13021 aaactccgcg cttaacacgt cgtcggtcgg cgcatcatcg ctggtcagca gtgccagcaa
    13081 cgttgccgca tccgctttca gcaagcggtc agctttattg tcgagcaaca aatcagcgag
    13141 acgcttaaga accgcgatgg gctgatcgtc attcatcgct acactcacca gcatggctgc
    13201 cgtttcgccg tccacatcaa aagcatttgc cgcacggctt accgcaatcg cgctacgcag
    13261 attgccttcg gcgctatcgc tcagccagat gccctgtccg aggttcagcg gttgttcatt
    13321 gatggctttg gtgacgaaag tggcgtcaac tgcccccgcc tctttcagac gcgcagcgtt
    13381 cagcgcctga agagtcagca gatcgctggc gacgatatcc agtgtcagca tttcgttgtc
    13441 gagcttcagc tgctcactct gcttttcgcc catcagcaat gcgcgaagtt cttctgctgt
    13501 tgttgctgac ttcagttgtt cagcaacgga atcatcactc agtacgtggg tcagctggcg
    13561 tagcaggccc agatgttcat ccgagctggc agcaataccg atcgccacgt acgctacctg
    13621 accgtcaccc caggtgacgc cttccgggaa ctgaaatacc tgaacgccgg ttttcagcac
    13681 ctgatcgcgg gtgtcggtgg tgccgtgtgg aatagcaata ccattgccga ggaacgttga
    13741 ggtttgctgt tcgcgcgcca gcatgccatt gacgtagcct tctgctacgt taccggcctg
    13801 caccagcgcc gcagcgacct ggcgaatcgc ctcttctttg tctccggcct tttcgcccgg
    13861 atggatgtcc tgtacggata actggaacat agttctcctc tcttgctgaa ttgaaacgat
    13921 tcagcctcta tgagaaaaaa agcgccaacc tggcttaggg ttaaagacaa gatcgcgctg
    13981 aaacgtttca agaaagcata atacttctga ttcagcacgc aaggaaagcc gtaaagttat
    14041 gagcaaaagt ttgatgtgct gcacaatttt ttgtcatttt tcctaattgc tgatgggaaa
    14101 atccgttatc agcaatttca tttcagcagc gtcagcagcc aatcagcaac agtgaaatgg
    14161 cattttgatt attcgtggcg cttttgattt cgctgtctgt ttattttctg acaagcagcg
    14221 taaactccgc gtcttcctct tccagtgatc gaccagcatg cataactccc ccgcagtctc
    14281 cagcgcgaaa tcgtttgacc tgacctcgac ggcgttttta atcgttgcct ttctcaccgg
    14341 tattgcgggc gctctgcaaa ccccgacact cagtattttt cttactgatg aagtacatgc
    14401 ccgtccggcg atggtgggat tcttctttac cggcagcgct gtcattggga ttctggtaag
    14461 tcagtttctc gccgggcgct ctgataagcg cggcgatcgc aaatcgctga ttgtcttttg
    14521 ctgcctgtta ggcgtgctgg cctgcaccct ttttgcctgg aatcgcaact actttgtttt
    14581 gctattcgtt ggcgtctttc ttagcagctt tggctcgacc gctaacccgc aaatgtttgc
    14641 ccttgcccgt gaacatgccg acaaaaccgg acgtgaagcg gtgatgttca gctctttttt
    14701 acgcgctcag gtttcactgg catgggtcat tggcccaccg ctggcttatg ccttagcgat
    14761 gggtttcagc tttacggtaa tgtatctgag cgcagcggta gcatttattg tttgcggcgt
    14821 gatggtgtgg ctgtttttac cgtcgatgca aaaagagctt ccgctggcga ccggcacggt
    14881 tgaagcgccg cgccgtaacc gtcgcgatac gctgctgctg tttgtcattt gtacattgat
    14941 gtggggctcg aacagcctgt acatcatcaa catgccgcta tttattatca acgaactgca
    15001 tcttcccgag aaactggccg gtgtgatgat ggggaccgcc gccgggctgg aaatcccgac
    15061 gatgttgatt gccggatatt tcgccaaacg tctgggtaag cgtttcttaa tgcgcgttgc
    15121 tgccgtgggt ggcgtctgtt tttacgcagg aatgctgatg gcgcattcac ctgtcattct
    15181 gttgggcttg cagctgctaa atgctatttt tattggcatt ctgggcggca tcgggatgct
    15241 ctattttcag gatctgatgc ccggtcaggc gggttcagcc accacgctct ataccaacac
    15301 gtcgcgcgtg ggctggatca tcgcaggatc agtggcggga atcgtcgccg agatctggaa
    15361 ttatcacgct gtgttctggt ttgcgatggt gatgattatc gccactctgt tttgcttact
    15421 gcggattaaa gatgtttaag gcgcggtcag catttccaga tcgataagcc aggtcatggc
    15481 ctgctgacgc gagttaccgc acatttcagc gctggcttgt aagcctgcac agactttcgg
    15541 tcgcagtggc gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa tgcagggcgt
    15601 attggcgggc ttgccatctg gcataccggg aatagggctg gaaattgaag gggcagtgca
    15661 acacgccccg caacccggac ggcattccat acgttgttct cttaaggtta accaccggat
    15721 tgatgagcgc gcacagtacc accttttttg caccagcaaa agtgcgaata ccacttgccc
    15781 gaaaggcccg tcgcgagtac tttgtcgcga tatttttgac attttcgact acaggaattt
    15841 ttcgatgcca agagcgaacg aaattaaaaa aggtatggta ctgaattaca acggcaaact
    15901 gctgctggtt aaggatattg atattcagtc gcccactgcc cgcggtgccg ctacgctgta
    15961 caaaatgcgt ttttctgatg tccgtaccgg gctgaaagta gaagagcgtt tcaaaggtga
    16021 tgatatcgtt gacaccgtga cgctgacccg ccgttacgtt gacttctcct atgtcgatgg
    16081 caacgaatat gtctttatgg ataaagaaga ctataccccg tataccttca ccaaagatca
    16141 gattgaagaa gagttgctgt ttatgccaga gggcggcatg ccggacatgc aggtgctgac
    16201 ctgggacggt caactgctgg cgcttgagct gccgcagacc gttgatctgg aaatcgttga
    16261 aaccgcacca ggcatcaaag gggcatctgc cagcgcccgt aacaaaccgg cgacattgag
    16321 cactggtctg gtgattcagg taccagaata cttaagcccg ggcgaaaaaa ttcgtatcca
    16381 tatcgaagaa cgccgttata tggggcgtgc tgactaactt cagccgcatg cagaaaaggg
    16441 atagctcagg ctgtcccttt tttaatttat tataccaatc ttctattttg cgcttcctga
    16501 acaccttacg ccctcaattt tcactcgttg attgatcgcc ctcacacttc atcgcattaa
    16561 caatccagac caatttcaat tgctgtcata taactttaca ctgtcattgt taatttatcg
    16621 ttactaagac gtgactccta tgaagacaat tgcctccgtt acgctcccgc atcatgtaca
    16681 cgctccacgc tacgatcgcc agcagttgca atcacgtatc gttcattttg gctttggcgc
    16741 ctttcaccgc gctcatcagg cgttactgac cgatcgtgtg ctgaatgccc agggcggcga
    16801 ctgggggatc tgtgaaatca gcttgttcag cggtgatcaa ctgatgagcc agctccgcgc
    16861 acagaaccat ttatataccg tgctggagaa aggtgcggac ggcaatcagg tgataattgt
    16921 cggtgccgtt cacgaatgcc ttaatgcaaa actggattcc ttagcggcaa ttattgagaa
    16981 attttgcgag ccacaggtgg caattgtttc cctgacgatt accgaaaaag gctattgtat
    17041 tgacccggcc accggtgcac tcgacaccag taatccgcgg attattcacg atctacaaac
    17101 ccctgaagaa cctcactccg caccgggtat tctcgtcgaa gcactgaaac gccgccgtga
    17161 gcgcggcctt acaccgttta ccgtgctctc ctgcgacaat attcccgaca atggccatgt
    17221 ggtgaaaaac gcggtgctgg gaatggcaga aaaacgttcg ccagaactcg ccgggtggat
    17281 aaaagaacac gtcagttttc cgggaaccat ggtcgaccgc attgttccgg ctgcaaccga
    17341 cgaatcactg gcagaaatca gccagcatct gggggtaaat gatccctgcg cgattagctg
    17401 cgaaccgttt atccagtggg tggtggaaga taacttcgtc gctgggcgtc ctgcctggga
    17461 agtcgcaggt gtacaaatgg tgaatgatgt cctgccatgg gaagagatga aactgcgtat
    17521 gcttaatggc agccactctt ttctcgctta tctgggttac ctttcaggat tcgcccatat
    17581 cagtgattgc atgcaggatc gcgcatttcg ccatgccgcc agaacattaa tgctgaatga
    17641 gcaagcgccg acactacaaa ttaaagatgt cgatttaaca caatatgcgg acaagttaat
    17701 tgcacgtttt gctaatccgg cgctgaaaca taagacctgg caaatcgcaa tggatggtag
    17761 ccagaaatta ccgcaacgta tgctggcagg tattcgcata catctggggc gcgaaacgga
    17821 ctggtcgttg ctggcattag gcgttgctgg ctggatgcgt tacgtcagcg gcgttgatga
    17881 tgccggaaat gccattgatg ttcgcgatcc gcttagcgat aaaattcgcg aacttgttgc
    17941 gggcagcagc agtgaacaac gcgtaaccgc cctgctttcc ctgcgtgaag ttttcggtga
    18001 tgatctgcca gataacccgc attttgtgca ggccatcgaa caagcctggc aacaaatcgt
    18061 acaattcggc gcacatcagg cgctattaaa caccctcaaa atttaacgat ttctgcggtt
    18121 aaagcggatg aagctcacct tcgtccgctc tccccttctc ttttctgcct tttttagcca
    18181 ggattaacgc tcagttaact taccagagta ttgcggagcc gttgtgacca ggaccaacct
    18241 catcaccggt tttctcggca gcgggaaaac cacgtcgatt cttcatctgt tagcccataa
    18301 agatcccaac gaaaaatggg cggtactggt taatgaattt ggggaagtcg gaattgatgg
    18361 tgctttgctc gccgatagcg gcgcattgct gaaagagatc cccggcggct gcatgtgctg
    18421 cgttaatggt ttacccatgc aggtagggtt gaatacctta ctgcgtcagg gaaaaccaga
    18481 ccgcttgttg atagagccga ccgggctggg ccatccgaaa cagatcctcg atctgttaac
    18541 cgcaccagtc tatgaaccgt ggatagatct gcgcgccacc ttgtgcattc tcgatccacg
    18601 cctgctgctg gacgaaaaaa gcgccagcaa tgaaaacttc cgtgaccagc tggctgccgc
    18661 agacatcatt gtcgccaata aatccgaccg tgcgacgccc gaaagtgagc aagcgctaca
    18721 gcgttggtgg cagcaaaatg gtggcgatcg acaattaatt cacagtgagc atgggaaagt
    18781 tgacggtcat cttctggatt tgccgcgtcg caatttagcc gagttgcccg ccagcgccgc
    18841 gcattctcat cagcatgtcg tgaaaaaagg gttagcagcg ttaagcctgc cagagcatca
    18901 acgctggcgt cgcagtctga acagcgggca aggatatcag gcctgcggct ggatattcga
    18961 cgctgatacg gtattcgaca ccattggcat tctggaatgg gcgcgacttg caccggtgga
    19021 acgcgtcaaa ggcgtgctgc gtattcccga agggctggta cgaatcaacc gtcagggcga
    19081 tgacctgcac attgaaacgc aaaacgttgc gccaccggac agccgtattg agctgatttc
    19141 cagcagcgaa gctgactgga atgctttgca gagcgcgctg ttgaagcttc gtttagcgac
    19201 taccgcgtaa ggttgcctgc gtttttcagt aagataatta gagaaaatat gattaaaaat
    19261 ttgccgcaaa tagtgttgtt gaatattgtc ggcctcgcgc tgtttctttc ctggtatatt
    19321 cccgttaatc atggattctg gttgccaatt gatgcggata ttttttattt ctttaatcag
    19381 aaactggtcg aaagtaaggc ctttttgtgg ctggttgcat tgaccaacaa tcgcgctttc
    19441 gacggttgtt ctctgctggc gatgggaatg ttgatgctga gtttctggct gaaagaaaac
    19501 gcccctggca gacgacgtat cgtgattatg ggtctggtca tgctattaac tgcagtggta
    19561 ttaaaccagc tgggtcaggc attaattcct gtaaaacggg ccagcccaac attgactttt
    19621 accgatatta accgcgtcag cgaactgctc tctgttccca cgaaagatgc ctcacgagat
    19681 agctttcccg gcgatcacgg catgatgctg cttatttttt cggcattcat gtggcgttat
    19741 ttcggcaaag ttgcaggcct tatcgccctt attatttttg tggtttttgc atttcccaga
    19801 gtaatgattg gcgcacactg gtttactgac atcattgtcg gttcgatgac cgtgatattg
    19861 atcggtttgc cctgggtgtt gctgacgcca ttaagtgatc gattaatcac cttttttgac
    19921 aaatcactac cgggaaaaaa caaacatttc caaaacaaat aactcacagt aattaacatc
    19981 atcaggctta tttttatagt gaggataatc ctgatgatgc gcaccgtgct ttcatccatc
    20041 gcgcgcaaaa atcattctct aagtaaatga atggattgca tgcgtttcac tcaatcgtac
    20101 tttaattgac caatcccgct tattaacttt ctgtatcact ttttcttata aaaaatcatg
    20161 taaaaccgct cgccaagacc gcaccaatcg ggtaatctcg aactcgtttt gcctcggcgg
    20221 tagattatcc tcacagcata taattttgtg cgttagtcca cagatttggc cttaaggaat
    20281 tgtttcaaca tgcccaggta attagtctcg tgtcgcttgg cattttttta taacgatatt
    20341 tgtcgttaag gacttcaagg gaaaacaaac aacatggtca aatctcaacc gattttgaga
    20401 tatatcttgc gcgggattcc cgcgattgca gtagcggttc tgctttctgc atgtagtgca
    20461 aataacaccg caaagaatat gcatcctgag acacgtgcag tgggtagtga aacatcatca
    20521 ctgcaagctt ctcaggatga atttgaaaac ctggttcgta atgtcgacgt aaaatcgcga
    20581 attatggatc agtatgctga ctggaaaggc gtacgttatc gtctgggcgg cagcactaaa
    20641 aaaggtatcg attgttctgg tttcgtacag cgtacattcc gtgagcaatt tggcttagaa
    20701 cttccgcgtt cgacttacga acagcaggaa atgggtaaat ctgtttcccg cagtaatttg
    20761 cgtacgggtg atttagttct gttccgtgcc ggttcaacgg gacgccatgt cggtatttat
    20821 atcggcaaca atcagtttgt ccatgcttcc accagcagtg gtgttattat ttccagcatg
    20881 aatgaaccgt actggaagaa gcgttacaac gaagcacgcc gggttctcag ccgcagctaa
    20941 taaaccgttt ggatgcaatc ccttggctat cctgacgagt taactgaaag cactgcttag
    21001 gcagtgcttt tttgttttca ttcatcagag aaaatgatgt ttccgcgtct tgatccaggc
    21061 tatagtccgg tcattgttat cttttaaatg ttgtcgtaat ttcaggaaat taacggaatc
    21121 atgttcatac gcgctcccaa ttctggacgt aagctcctgc ttacctgcat tgttgcaggc
    21181 gtgatgattg cgatactggt gagttgcctt caatttttag tggcctggca taggcacgaa
    21241 gtcaaatacg acacactgat taccgacgta caaaagtatc tcgataccta ttttgccgat
    21301 ctgaaatcca ctactgaccg gctccagccg ctgaccttag atacctgcca gcaagctaac
    21361 cccgaactga ccgcccgcgc ggcgtttagc atgaatgtcc gtacgtttgt gctggtgaaa
    21421 gataaaaaaa cattctgttc atctgcgacc ggtgagatgg acattccact caatgaattg
    21481 attccggcgc tcgacattaa taaaaacgtc gatatggcga tcttacccgg cacgccgatg
    21541 gtgccgaaca aacccgcaat cgtcatctgg tatcgcaacc ctttgctgaa aaatagcggc
    21601 gtctttgccg ctctgaatct caacctgacg ccttctctct tttatagttc acggcaggaa
    21661 gattacgatg gcgtcgccct cattattggt aatactgcgc tatctacctt ttcttcacgt
    21721 ttgatgaacg ttaacgaatt aaccgacatg ccagtccgtg aaactaaaat tgcgggcatt
    21781 cctctgaccg ttcggcttta tgcagatgac tggacatgga acgatgtgtg gtacgcattt
    21841 ttactgggcg gcatgagtgg aactgtcgtt ggcctgctct gctattacct gatgagcgta
    21901 cgtatgcgcc ccggcagaga aatcatgacc gccatcaagc gcgaacaatt ttacgttgcg
    21961 tatcaaccgg tggtggatac acaagctttg cgagtaacgg gcctggaagt actgctacgc
    22021 tggcggcatc ctgtagcggg agaaattccc ccggatgcct tcattaactt tgccgaatcg
    22081 caaaagatga ttgtgccgct gactcagcac ctgtttgagt tgattgcccg cgatgccgca
    22141 gaattagaaa aagtactgcc ggtaggcgtc aaatttggca ttaacattgc gccggaccat
    22201 ctgcatagcg aaagctttaa agctgatatc cagaaactgc tcacgtcctt gcccgcacac
    22261 catttccaga ttgtgctgga aattaccgag cgcgatatgc tgaaagagca agaagccaca
    22321 caactcttcg cctggctgca ctcggtcggc gtagaaattg ctattgatga cttcggcacc
    22381 gggcacagcg cgcttatcta tcttgagcgt tttacgctcg attatctgaa aattgaccgt
    22441 ggatttatca acgccatcgg tacggaaacg atcacttccc ccgtacttga cgcggtgctg
    22501 acgctggcga aacgcctcaa tatgctgacg gttgctgaag gggtcgaaac gccggaacag
    22561 gcgcgatggc taagcgaacg cggcgttaat ttcatgcaag gctactggat tagtcgcccg
    22621 ttaccgctgg acgattttgt tcgctggcta aagaaaccgt atacgccgca gtggtaaggt
    22681 gtgcttacgt cccttattat tcatagtgaa agcatgccgg attgcggcta atgatgagta
    22741 aaaggaaatc cgttgcagat gattgtgcgc atactgctgc tgtttatcgc tctgttcacc
    22801 tttggtgtgc aggcgcaggc tatcaaggaa agctatgcct ttgccgtgct gggcgaaccc
    22861 cggtacgcgt ttaatttcaa ccattttgat tatgtgaacc ccgccgcgcc aaaaggtggg
    22921 cagataacgt tgtcagccct cggcaccttc gataatttca accgctatgc actgcgcggc
    22981 aacccgggcg cacgcaccga gcagctgtac gacacgctat ttacgacttc cgatgacgaa
    23041 ccaggcagtt attacccgct gattgctgaa agcgcacgct atgctgacga ttattcctgg
    23101 gtggaggtcg ctattaatcc gcgcgcccgt tttcatgatg gttcgcccat tactgcccgc
    23161 gatgtagagt ttacttttca aaaatttatg accgaaggcg tgccgcaatt tcgtctggtc
    23221 tacaaaggca ccaccgtcaa agccattgcg ccgttaaccg tgcgcattga gttagctaaa
    23281 cccggcaaag aagatatgtt gagtctgttt tcgctgccgg tatttccaga aaagtactgg
    23341 aaggatcaca aacttagcga cccgctcgcc acgcctccgc ttgccagtgg tccgtaccgc
    23401 attacgtcct ggaaaatggg gcaaaatatt gtctattccc gcgtgaaaga ttactgggca
    23461 gcaaacttac cggtaaaccg cggacgctgg aatttcgaca ccattcgcta cgattattac
    23521 ctcgatgata atgtcgcctt tgaagcgttt aaagcaggtg cctttgattt gcgtatggaa
    23581 aacgacgcca aaaactgggc cacgcgttat accggtaaaa atttcgataa aaaatacatc
    23641 atcaaagatg agcaaaagaa cgaatcagcc caggatacgc gctggctggc gtttaatatc
    23701 caacgtccag tattcagcga tcgccgggtg cgggaagcaa tcactctcgc ctttgacttt
    23761 gaatggatga acaaggcgtt gttttacaat gcctggagtc gcacgaacag ttattttcag
    23821 aataccgaat acgcggccag aaattacccc gacgccgcgg agctggtgct tctggcacca
    23881 atgaaaaaag atctaccgcc agaagtcttc acacaaatct accagccgcc ggtatccaaa
    23941 ggcgatggct acgatcgtga caacctgtta aaagccgaca aacttctcaa cgaagcgggc
    24001 tgggtgctga agggtcagca acgcgttaat gccacaacgg gtcagccact cagctttgaa
    24061 ttattgcttc ccgcaagcag caatagtcag tgggtattgc cgttccagca cagcctgcaa
    24121 cggctgggta tcaacatgga cattcgcaag gtggataact cgcaaatcac taaccgcatg
    24181 cgcagtcgcg actatgacat gatgccgcgc gtatggcggg cgatgccgtg gcccagttcc
    24241 gatttacaga tttcctggtc atcggaatat atcaattcca cttataatgc ccccggcgtg
    24301 caaagcccgg ttatcgactc gctgatcaac caaattattg ccgcgcaggg aaataaagaa
    24361 aaattactgc cgttggggcg agcactggat cgcgtattaa cgtggaatta ttacatgctg
    24421 ccaatgtggt acatggcgga agaccgtctc gcctggtggg ataaattctc ccagccggcc
    24481 gtgcgcccca tctatagcct cggtatcgat acctggtggt atgacgtcaa taaagcggcc
    24541 aaactgccgt ccgccagcaa acagggagag tagatgggcg cttacctgat tcgccgtctg
    24601 ttgctggtga tcccaacatt atgggcgatt atcaccatca actttttcat cgtgcaaatt
    24661 gcgcctggcg gtccggtcga ccaggccatc gccgccattg agtttggtaa tgccggagta
    24721 ttacccggcg caggcggtga aggtgttcgt gccagccatg cgcaaacggg tgtcggcaat
    24781 atcagcgaca gtaattaccg tggcggacgc ggattagatc cagaagtgat cgctgagatc
    24841 actcatcgct acggttttga taagccgatc cacgaacgtt acttcaaaat gctctgggac
    24901 tacatccgct ttgattttgg cgatagcctg tttcgcagcg cctcggtgct gacgctgatt
    24961 aaagacagtc tgccggtttc catcaccctc ggattgtgga gcacgctgat tatctatctg
    25021 gtgtcgattc cgttaggcat tcgcaaagct gtttataatg ggagccgctt tgacgtctgg
    25081 agtagcgcat ttatcatcat cggctacgcc attccggcct ttttgtttgc catcctgctg
    25141 attgtcttct tcgcgggcgg cagctatttc gacctgttcc ctctacgcgg cctggtttcc
    25201 gctaactttg attcgctgcc gtggtatcag aaaatcaccg attatctgtg gcatatcacg
    25261 ctgccggtgc tggcgacagt gattggtggc tttgcggcgc tgaccatgct gacaaaaaac
    25321 tcattccttg atgaagtgcg caagcaatac gtggtgaccg cgcgtgcgaa aggggtaagt
    25381 gaaaaaaata ttctctggaa acatgtgttc cgcaacgcca tgctgctggt gattgccggt
    25441 tttccggcga cgtttatcag catgtttttt accggctcgc tgctgattga ggtgatgttt
    25501 tcactcaatg gtctgggctt actgggctac gaagcgaccg tctcgcgcga ttatcctgta
    25561 atgtttggta ccttgtatat tttcaccctg attggcctgc tgctgaatat tgtcagtgat
    25621 atcagctata cgctggttga tccgcgtatt gattttgagg gacgttaatg tcgcgactca
    25681 gccccgtcaa tcaggcccga tgggcgcgtt ttcgtcataa ccgtcgcggc tactggtcgt
    25741 tatggatttt cctcgtcttg tttggtttga gtttgtgttc tgaacttatc gccaacgata
    25801 aaccgttgct ggtgcgttat gacggcagtt ggtatttccc gttattgaaa aactacagcg
    25861 aaagcgattt tggcggcccg ctggcaagtc aggctgatta tcaggacccg tggctgaaac
    25921 aacggctgga aaataacggc tgggtactgt gggcaccgat tcgctttggt gctaccagta
    25981 tcaactttgc taccgataag cccttccctt ctccaccctc ccggcaaaac tggctgggaa
    26041 cggatgccaa cggcggcgat gtgctggcac gcattctcta tggcacgcgg atctcggttc
    26101 tgtttggcct gatgctgact ctctgttcca gcgtgatggg cgtgctggcg ggggcgctac
    26161 aaggctatta cggcggtaaa gtcgatctct ggggacaacg ctttattgaa gtatggtcgg
    26221 ggatgccgac gctgtttttg attattttac tttccagcgt cgtacagcct aacttctggt
    26281 ggctgctggc aattactgtc ttgtttggct ggatgagtct ggtcggcgtg gtgcgggcgg
    26341 agtttttacg tactcgtaat ttcgactaca ttcgtgcggc acaggcgctt ggcgtcagcg
    26401 atcgcagtat catcctgcgt catatgttgc ctaatgccat ggtcgcgacc ctcacctttt
    26461 taccgtttat tttatgtagt tcgataacca ccctgacctc gctcgatttc ctcggcttcg
    26521 gtctaccgct cggttcaccg tcactcggtg aactgctgtt acaagggaaa aataaccttc
    26581 aggccccgtg gcttgggatc accgccttct tgtcggtggc gatattattg tctttgctga
    26641 tctttattgg tgaagccgtc cgcgacgcat ttgatcctaa taaggcggtg tagcatgacg
    26701 caaactctgt tagcgattga aaatttgtcg gtgggttttc gccatcagca aaccgtacgt
    26761 acagtagtca atgatgtttc actacagatt gaggctggcg aaacgctggc gctggtgggt
    26821 gagtcaggtt caggcaaaag cgttaccgcg ctgtcaattt tacgcctgct cccttccccg
    26881 ccggttgaat atctctccgg cgatattcgt tttcatggcg aatcgctgct tcatgccagc
    26941 gatcaaacgt tgcgcggtgt acgcggtaat aagatcgcca tgatttttca ggaaccgatg
    27001 gtgtcgttaa atccattgca taccctggaa aaacagcttt atgaagtgct ttcactccac
    27061 cgcgggatgc gtcgggaagc ggctcgtggc gaaattctta actgccttga tcgcgttggt
    27121 atccgccagg cggcaaaacg gctgacagat tatccgcatc agctctccgg cggcgaacgc
    27181 cagcgggtga tgattgcgat ggcgctgtta acgcgaccgg aattattaat tgccgatgaa
    27241 ccgaccaccg cactggacgt ctctgtccag gcgcagattt tacagctgtt gcgcgaactg
    27301 caaggcgagc tgaatatggg catgctgttt attactcata acctcagcat tgtcagaaaa
    27361 ctggcccacc gcgtggcggt aatgcaaaac ggtcgctgtg tcgagcaaaa taacgccgct
    27421 acgctatttg cctcacccac tcatccttac acacaaaagc tactcaacag tgaaccgtca
    27481 ggcgacccgg tgccgttgcc agaacctgcc tcaacgttgc tggatgttga acagcttcag
    27541 gttgccttcc ccattcgcaa agggattttg aagcgcattg tggatcataa tgtggtggtg
    27601 aaaaacatca gttttacgct acgagcgggt gaaacactgg gtttagtggg cgagtccggt
    27661 tccgggaaaa gtacgacggg actggcgctg ctgcgactga ttaattctca gggcagcatc
    27721 atctttgacg gtcagccatt gcaaaattta aatcgccgcc agctgttacc tattcgtcat
    27781 cgcattcagg tggtatttca ggatccaaac tcctcgctca acccacgact caacgttttg
    27841 cagattattg aggaaggctt acgggttcac cagccgacgc tttctgccgc acaacgcgaa
    27901 caacaagtga tagccgtgat gcatgaagtg ggattagatc ctgaaacacg ccaccgttat
    27961 ccggcggagt tctctggtgg tcagcgacaa cgtattgcga ttgccagggc attaattctt
    28021 aagccctcgc tgatcatact tgatgaaccg acatcatcac tcgacaaaac ggtacaggcg
    28081 caaatattga cgctattgaa atcattgcaa caaaagcatc aactggccta tttgtttatc
    28141 agccacgatt tgcacgttgt ccgcgcgtta tgtcatcagg ttatcgtact gcgacaaggg
    28201 gaagtagtgg aacaaggacc gtgcgcgcgc gtgtttgccg caccgcagca ggagtatacg
    28261 cgtcagctac tggcgttgag ctgacgctta aaaaggattg tagtttgaaa agggttcggc
    28321 aattgccaca ccaaaatttt tcaatcggca tgttgcagca aactcgtcct ggcgatttac
    28381 aaacaggcac ggctcacctt cacactccag caccgaacat ggaacttcga tgtcgcttaa
    28441 cgcctggctt agtttgtaca tcaccgacca tgcattatca ccatccgggc taagcaattt
    28501 aagcgccacc aggctgttgt caccgcacgg tccattttgc ggaatcggtt caaccttcga
    28561 acctgcgaaa ccagcagacc agcgaaagtt ctgcggtaga cgatgaacga ttgagagttg
    28621 taatgaagtc acttattttc cccggaagta catttacttc acaatttgtt tacatcaatt
    28681 ttaacacatc atcaacaatc cgtctttata cagatcgtaa agattgatgc ctcgcctgcg
    28741 tggcctctat ggctctgatt taagtaatat ctgcgggcgt ggtcacgttt gcggggctat
    28801 atgtacccgt ttctgcgatc taactcaacc tttttaacta caatgatgtg actttttaca
    28861 taaattgatt ttacataaaa taaacatata tcgggggaat gatagatttg tggttgacat
    28921 gcatcaacaa aggaagcctt ttagcttcct cgttgtgcaa tagatcaccg ttttttcggt
    28981 cgactggcgt acagacagaa gagaatggag ctggttgcgc agaatgcaat tgaccaaatc
    29041 atcggccatg cagagttaaa ggtcgcaaga gaaagcaatg cgccaacaat tgcccctatg
    29101 ccaaaacgga aggttcctgc cagcgaagat gccgttcccg ccatatgggg aaactcatca
    29161 agaatgaccg ccatcgcatt ggatgacacc atcgacacgc agcccacaaa cgccgcaacg
    29221 ccaaccacca gcgaccagaa tcccagcccc agcagcgcac tgatgaccat ccacgccgcc
    29281 ataataaact gtatccacaa ccccgagcgg aacatattta acgcgccaat acggcggacg
    29341 aagcggctgt taaaaatggt catcacgaac aggaaaacaa tgtttagtgc aaagtaataa
    29401 ccaaagtttt ccggcgcgac gtggttaatt tcaatgtaga caaacggtcc ggcgctcagg
    29461 aacgagaaca ttccggcaaa gctgaaaccg ctggccagca tataactcag gacacgctta
    29521 tgacggaaca gcgccgcaaa gttaccaata gtggtacgaa tgtgaaaagg ctggcgacgc
    29581 tccggtggta aggtttcttt gatcagaaag aagatcattg ccgaagccag aatcgccgcc
    29641 acggcgagga tccagaagat gtaatgccag ctcagccaca ccagcaccca gccgccaact
    29701 atcggtgcca tcagcggtgc aatggttgtc accagcatga caaacgacat catccgcgag
    29761 aactcttctt tcgggtagat atcacgcatc aaagcgttga taaccacgct ggccgcagcc
    29821 gccgccagcc cgtggaagaa acgcatcaca atcagctgat cgatggtttg tgccaacgca
    29881 cacgccaccg cggcggcggc aaacaccagc gtaccgccaa gaaccaccgg cttacgcccg
    29941 aagctgtctg ccatcggccc gtagattaac tgccccaacg caaagcccag aatataagta
    30001 ctgagggtca tctgcgtact gcccgccggt acgccaaact gcgctgaaat taccggtagc
    30061 gcgggcagat acatatcaat cgacagcggc atcaacatgg ccagcaggcc aaggataaaa
    30121 acaatagcaa acgacgaatg ctgtcgggtg gtcacaacgg gctcctgaaa gtcattgaaa
    30181 agttagacga cgctggcaat ttcttcttca gttaacggac gatattcacc gggggctaaa
    30241 tcagcatcca gcgtaatgcc gccaatacgt tcacgatgca gctcaaccac atggttaccc
    30301 acgacggcga acatgcgttt cacctgatga taacgccctt cgctgatggt cagacgaacc
    30361 tgcgttgggg taatcacttc cagcaccgcc ggcttagtga gatctttttc gttatgcagc
    30421 tgcacgcctt tagcaaattg ctctgccgta tcgtcagcta cgggtgattc cagcgtcacc
    30481 agataggtct tctcgcaatg atggcgcgga gaagtaatgc ggtgcgacca ctgaccatca
    30541 tctgtcatca gcaccagacc ggtggtatcg atatccagac gccctgcggc gtgcagtttc
    30601 caggctaccg gttcatcaag aaaatagagc accgttgggt gatcagggtc gtccgtggag
    30661 caaacgtagc cctgaggctt attgagcatg aagtaacgtg gaccgtgttg ctgcgccagc
    30721 gggttgccat cgtaagcgac atcatgttca ggaagcagtt tgaacgctgc attacggacg
    30781 atttcgccat cgacggtgac acgattgccg cggatttcac gcccggcaat agcacggcta
    30841 acgccgagtt gctgtgcgat aaatttatca agtcgcatgt gtgtgatttt gcctgtaaaa
    30901 aacggaggtc gggtaatgac ccgaaaatct gaactgttgt ctgcccagta tagcggtcta
    30961 ataacatccc tcaagggaaa aagatccatg gcatactatt agcagaataa tctacctacg
    31021 cgagaccatg atttttacac ttcgcccata tcagcaagaa gccgtggatg ccacgctcaa
    31081 ccattttcgt cgtcataaaa cccctgccgt tatcgtgctg cctactggtg caggtaaaag
    31141 cctggtgata gcggaactgg cgcggctggc gcgtggtcgc gtgctggtgc tggcacacgt
    31201 taaagaactg gtggcgcaaa accatgcaaa atatcaggca ctgggactgg aagccgatat
    31261 ttttgccgcc gggctaaagc gcaaagagag ccacggtaaa gtggtatttg gcagcgtgca
    31321 gtcggtcgcc cgtaatcttg atgcctttca gggtgaattt tcgctgttga ttgtcgatga
    31381 atgtcaccgt attggtgatg atgaagagag ccagtatcag caaatcctca ctcacctgac
    31441 aaaagtgaat ccccacttac gcctgctggg gctgactgcc acgccttttc gattgggcaa
    31501 aggctggatc taccagttcc attatcacgg catggtacgc ggcgatgaga aagccctttt
    31561 ccgtgactgc atttatgagc tgccgctgcg ttatatgatt aaacacggct atctgacgcc
    31621 gccagaacga ctggatatgc cagtagtaca atacgatttc agccgcttgc aggcacagag
    31681 taacgggctg ttcagcgaag ctgatctcaa ccgtgagctg aaaaaacagc aacgcattac
    31741 gccacatatc atcagccaga tcatggagtt cgccgaaaag cgcaaagggg taatgatttt
    31801 cgccgcgacg gtcgaacacg caaaagagat tgtgggatta ctgcccgccg aagatgctgc
    31861 actgattact ggcgacaccc ccggcgctga gcgcgatgtg ttaattgaag attttaaagc
    31921 ccagcgtttt cgctatctgg tcaacgtcgc agtactgacc accggatttg acgccccgca
    31981 cgtcgatctt atcgccattc tgcgccctac cgaatcagtg agtctttacc aacaaattgt
    32041 cgggcgcggt ctgcgtctcg ctcctggcaa gactgattgc ttaattcttg attatgcggg
    32101 taatcctcac gatctctacg cgccggaagt tggtacaccg aaaggcaaaa gtgacaacgt
    32161 cccggtacag gttttctgcc ctgcctgcgg ttttgccaac accttttggg ggaaaacgac
    32221 cgccgacggg acattgattg aacactttgg tcgtcgctgt cagggatggt ttgaagatga
    32281 cgacggtcat cgcgaacagt gtgacttccg tttccgtttt aaaaattgcc cgcaatgtaa
    32341 cgcagaaaat gatattgccg cccgccgctg ccgcgaatgt gacaccgtac tggttgatcc
    32401 ggacgatatg ttaaaagcgg cgctacgact gaaagacgcg ctggtattac gctgtagcgg
    32461 catgtctttg caacatgggc acgacgagaa aggcgaatgg ttgaaaatca cctattacga
    32521 tgaagacggc gcggatgtga gtgagcgttt ccgtctgcaa acgcccgccc agcgaactgc
    32581 cttcgagcag ctttttatcc gcccgcatac gcgcacaccg ggcatccctc tgcgctggat
    32641 caccgccgcc gatatcctcg cccagcaagc gttactgcga cacccggatt ttgtcgtcgc
    32701 ccgcatgaaa ggtcagtact ggcaagtgcg tgaaaaagtg ttcgattacg aaggtcgttt
    32761 tcgtcgggcg cacgaattac gcggttaatg gtagttttga ttgatgtata aggcgattga
    32821 gtatagaatc tcgcccgctt ttgcatacgc aaagcagatc acttacctgt tgctgggtcg
    32881 cctgtagcag gattaattta agagagaaag aaatgtttac tatcaacgca gaagtacgta
    32941 aagagcaggg taagggtgcg agccgccgcc tgcgtgccgc taacaagttc ccggcaatca
    33001 tctacggtgg caaagaagcg ccgctggcta tcgagctgga tcacgacaaa gtcatgaaca
    33061 tgcaagctaa agctgaattc tacagcgaag ttctgaccat cgttgttgac ggtaaagaaa
    33121 tcaaagttaa agctcaggac gtacagcgtc acccgtacaa accgaagctg cagcacatcg
    33181 acttcgttcg cgcttaattg ctgaataagt tgtaaaaaac cccgctccgg cggggttttt
    33241 tgtatctgca gattatgcct gattacggta ttgctatttt ttgcaggcca gataaggcgt
    33301 tcacgccgca tctggcattc agaacacaaa accgattaat tgccgccaga cgtccggcgc
    33361 tgtaattggt cgcgcaaatt cggcggcgtg cctttaatgg tcaaggtatc ggttgctgga
    33421 tcccagaaga tgcgttcacc taacagcatc gcatcaaagt taatagtcaa gccaccgccg
    33481 ctaccggcaa acttcgtcaa ctgacgcagt gtgctacggt ctgccgggaa gctctcttcc
    33541 agttcgtagc ctttttcagc cgcaaactcc gtgaaactca cttcgctgac gcctgccagc
    33601 tctttcgaca gcgactccag ttcaatctct tcgcctgctt gcagctgctc gttacagtag
    33661 ctataaacct gctggcgcac attctgccgc tcggctttat ccagttgcgc ttgtgcagtg
    33721 aaatcatcca ccgcttgcag caagccacgg ttttgcgcct ttgcattcag cccttcgctg
    33781 gcaccaagga aatccataaa gaaatcggcg accttgcgcc ctacccgccc tttcaggaaa
    33841 gttaaatagc gggtcgattc cgggttggtt tcccattcgg ttaaatcgat gcgcgcgacg
    33901 atatccgcat ggttgatatc gagatagtgc gttgggttga tgtcgagatt ttcgttaacg
    33961 cgcatactgc tcaggttgct caggactgcc accagcagat actccaccgc cagataacgg
    34021 tagtggcaaa acagcacgaa tccaccgtca gcaaaaggat atttcgctaa ttcgtcacgc
    34081 aggcgaccag ttgccgcgcg gctaaatgcc agaaagtcct cttcgccctg acgctgtaag
    34141 cgcagcgtct gcgccagttc actctcttcg ctaaacaagc cgtaagcttt atttttggcg
    34201 ctatagaccc gatgcagttc tgccaccatc tcaacgacgg tttctgtcgg ttccagcaat
    34261 gaatcgcgca acaccagctc aaggttctgc tcatcacgct tgataagctg gtgcagagca
    34321 atctggttga tatccagact catgataaac tctcctttaa gaccgggcgg tattcaacca
    34381 ccgccggtta agcgacgcaa taaaagatgg cggtcatttt acagaaggca gcgtaacgag
    34441 aatgatttaa cgcataaaaa agcagaaaaa aaaccgttgc tacggtaata tgttgccctt
    34501 tcatcaacta actgatttcg atttatgcca caaatttccc gctacagtga tgaacaggtt
    34561 gaacaattgc tcgcagagct gctcaacgta ctggaaaaac ataaggctcc gaccgacctt
    34621 tccctgatgg tgttaggcaa catggtcact aaccttatca acaccagcat tgccccggcc
    34681 caacgccagg caattgccaa ctcttttgcc cgcgccttac agtcctctat caacgaagac
    34741 aaagcgcact aagggaaaca gataacaggt tatggtaact catcgtcagc gctaccgtga
    34801 aaaagtctcc cagatggtca gttgggggca ctggtttgca ctgttcaata ttctgctttc
    34861 gctcgtcatt ggcagccgtt acctgtttat cgccgactgg ccgaccacgc ttgctggtcg
    34921 catttattcc tacgtaagca ttatcggcca tttcagcttc ctggtgttcg ccacctactt
    34981 gctgatcctc ttcccgctga cctttatcgt cggctcccag aggctgatga ggtttttgtc
    35041 cgtcattctg gcaacggcgg gaatgacgct attactgatc gatagcgaag tctttactcg
    35101 tttccatctc catcttaatc ccatcgtctg gcagttggtt atcaacccag acgaaaatga
    35161 gatggcgcgc gactggcagc tgatgttcat cagcgtgccg gttattttat tgcttgaact
    35221 ggtgtttgcg acgtggagct ggcaaaagct gcgcagcctg acgcgtcgtc gacgcttcgc
    35281 gcgcccgctg gccgcattct tatttatcgc ctttatcgcc tcgcatgtgg tgtatatctg
    35341 ggccgatgcc aacttctatc gcccgatcac catgcagcgc gctaacctgc cgctttcgta
    35401 cccgatgacg gcgcgacgtt ttcttgagaa gcatggcctg cttgatgcgc aggagtatca
    35461 acgccgtctt attgagcaag gtaatccaga cgccgtttcc gttcagtatc cgttaagcga
    35521 actgcgctat cgcgatatgg gcaccgggca gaatgtcttg ttgattactg tcgatggcct
    35581 gaactactca cgcttcgaga agcagatgcc tgcgctggca ggttttgctg agcaaaatat
    35641 ttcgttcacg cgccatatga gctccggcaa cactacagac aacggcatct ttggcctgtt
    35701 ctatggcatc tcgccgagct atatggacgg cattctgtcg acccgtacgc ctgcggcatt
    35761 aattactgcg cttaatcagc aaggctatca gctggggtta ttctcatcag atggctttac
    35821 cagcccgctg tatcgccagg cattgttgtc agatttctcg atgccgagcg tacgcaccca
    35881 atccgacgag cagaccgcca cgcagtggat caactggctg ggacgctacg cacaagaaga
    35941 taaccgctgg ttctcgtggg tttctttcaa tggtactaac attgacgaca gcaatcagca
    36001 ggcatttgca cggaaatata gccgggcggc aggcaatgtc gatgaccaga tcaaccgcgt
    36061 gctcaatgca ctgcgtgatt ctggcaaact ggacaatacg gtggtgatta tcactgccgg
    36121 tcggggtatt ccactgagcg aagaggaaga aacctttgac tggtcccacg gtcatctgca
    36181 ggtgccatta gtgattcact ggccaggcac gccggcgcag cgtattaatg ctctgactga
    36241 tcataccgat ctgatgacga cgctgatgca acgcctgcta catgtcagca cacctgccag
    36301 cgaatattcg caaggtcagg atttgttcaa ccctcaacgc cgtcattact gggttaccgc
    36361 agcggataac gatacgctgg caattaccac cccgaaaaag acgctggtgc tgaacaataa
    36421 cggtaaatac cgcacttaca acttacgtgg tgaaagagtg aaagatgaaa aaccacagtt
    36481 aagtttgtta ttgcaagtgc tgacagacga gaagcgtttt atcgctaact gattaattat
    36541 aaatcagtta gcgaaatatc ttacttgcaa tcggtgtgga aaacggtagt attagcagcc
    36601 acgagtcggc acgtagcgca gcctggtagc gcaccgtcat ggggtgtcgg gggtcggagg
    36661 ttcaaatcct ctcgtgccga ccaaaaatcc caaaaaaacc aaccattacg gttggttttt
    36721 ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt ttattttctg
    36781 ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattctc cccctgcgcc
    36841 attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg accttatccc
    36901 agcctgataa acgccacggg ctccagccac atcattatta aatttgccat cgtcattaac
    36961 tttcacctgg ttagaatcgg cgtattcctg acgtacggcg gctttcagcc agggttccag
    37021 cgtcgtacca ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt cagcgcgtaa
    37081 tatccgggta tttcccacat cagcgcgcat gccgtttgat aacgtgtagt cctgaccatc
    37141 tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt caacccaacg
    37201 gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa acgctgttgc
    37261 cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca ccaccccatc
    37321 aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat agctatcgat
    37381 attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa agaacaagcc
    37441 gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg tcaggcccgt
    37501 caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg tgttaattgc
    37561 cgaactccac atcgccgtat cgtaactaac gccttttacg ctaccaagac gctcacgcac
    37621 ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggcc gccatattca gcacatcagt
    37681 ggttgaaggg gtaatttgcg cgcgattctc tgccaaactc cagctatggt tgccattatc
    37741 cagcaaggta tattcatacg taccgatatc aacaacgcct ccagcattgc ccaacgtaaa
    37801 tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg ccgggctggc
    37861 accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga cgttgagctg
    37921 atcgctctga tgattagcca tatcggtacg catataaaaa ttgccgtttc ccgacagggt
    37981 attggtggtc aacgtaatga aattttccgc acttgccgtc actgatgaac gagtcactgg
    38041 ctcatccatc ataatcacat ttgcgtcaga catcgcaagg ttggctaagt tgaggagggt
    38101 gttttggttc gtcgtagtga tatcggcaat gatggtccat acgctatttt cgagcgaaac
    38161 gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct cagaaaccac
    38221 taccttaccc accaacattg cgggtgcata atttatttgc ggcttcatga cctcaagaat
    38281 gccatcattc cctctgactg aaacgttaac cattgtgcca gcccagacgt tagcgcggcc
    38341 attgttgata accatatttt cagagatggc ttttgaggaa tacgtgatac ttccattctg
    38401 atctgatcga ccgagatcat acaccgcatt tgcctgcact ctcgtcccag tatcctttcc
    38461 cagcgattgc atagtggcat gctcatcaag gatagtatca atggcctgcg tgtcctccat
    38521 aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat cttttatact
    38581 aaattgacct ttactgtttg taccactcac ttccagcgca ttcgttgaga caattaattt
    38641 tccgcctgct ttctgatcta ccgcaatcgc taatcccccc tctttaacct ccagtaatcc
    38701 tcccttttcg atagcggcaa ggacggtaat cccatctttt tcaacgatta agcgcccgcc
    38761 tttaatcgtg ggatcattcg ctgcgccata aacctgtagc acaccattat tgagggtagt
    38821 ctggtttgcc gcgccaccca tttgaacaat ttgataaccg tcatttaata ctgagtattc
    38881 tgcggtgcca ttcatttcaa tgtactgata cccgccgtta atttcagtat tactacttat
    38941 accaaattct ttaatatgct gttctccgcc aggattgatt ttcgtattat tggcggtccc
    39001 ataaaccagc tggatatctt tttccaggac aacaccatca actgtctcac catactcaac
    39061 tgttgatgcc aatgctatgg gtgaatataa cgctgtagcc accagaacag atattgcgga
    39121 acgacaaaga gaaacagaac cagattgatg cattgagctt tcatcctatg aaattaattg
    39181 cagttaaaag cattgggtac agaaaatacc catagctcca tacccggagt cagtttttaa
    39241 aaactgttta aagaaatgca caagtattgt gattgatttt ttagttgttt ttcttgatga
    39301 gaagctgatg caaaattccg tctttataat gaaaatgatg ccaaagcgaa cgacaaggtt
    39361 gtagttttca ctacatgtcc atacataaaa tggggtaaca ttcacgcgcc tggtagcgtt
    39421 accaacgcta cgctcaaaca taatgattct aataaaacct caggagacta ctatgcctga
    39481 agcaacgcct tttcaggtga tgattgtgga tgatcatcca cttatgcgac gcggtgttcg
    39541 tcagttactg gagcttgatc ctggctttga agtggtcgcc gaagcgggcg acggcgcgag
    39601 cgcgatcgat ctggcgaata gactggatat cgacgtgatc ttgctggatc tcaatatgaa
    39661 aggtatgagt ggcctggata ccctcaatgc cttgcgcagg gatggcgtta ccgcgcaaat
    39721 tattatcctg accgtatccg atgcctccag cgatgtcttt gcgctgatag acgcaggcgc
    39781 agacggttat ctgttgaaag acagcgaccc ggaagtattg ctggaagcga ttcgtgccgg
    39841 agcgaaaggc agcaaagtct ttagcgaacg cgtcaatcag tacttacgtg aacgtgaaat
    39901 gtttggcgcg gaagaagatc ccttcagcgt gctgacggag cgcgagctgg atgttctgca
    39961 cgagctggca caggggctgt caaataaaca gattgcctcg gtgttgaata tttccgagca
    40021 gacagtaaaa gtacatattc gcaatctgct gcgtaaactc aatgtccgct cacgcgtggc
    40081 ggccaccatt ctgttcctgc aacaacgcgg ggcacaataa aaatagcccg atggcatcac
    40141 catcgggcct ctttttattt actctcctgc ggcgacaaat gttgcatcgc ctgcgcgata
    40201 ctacgttcaa tcaccgcacg gcgagtatcg ttggcaggta agagtttcaa catcatctcc
    40261 cacgcggcaa cggcttcgcc aaatcgctgc tgctcaaagg cgttaaacgc atacatgctt
    40321 aacacacgaa tattgctatg gtccgttctc accaactggc gcagcagttc gccgccgaga
    40381 cggttgtcgt tgggatcaga tgaacgtgtc aacgcttcag cgtatcccag tgcagcatcg
    40441 ctgttcttcg gatcgaggcg atacgcagtg gcgtatgcat cggtggcgat actggcgtta
    40501 cccagcgcca tgccaacgcg gcccaacata atccagcctt ctatatcacc cggatttttt
    40561 tgcagttgag tacgcatccc cagcgcaagg cgcgacatct cttcttcgtt gagcggatcg
    40621 gctttcggat ccagcgccct gtccagcaac gccggagcct gtgccgtggc ctgctgccag
    40681 attttcacct gctgataatt gccagtctgg tagtagctga cgccagccac aattagcgcc
    40741 accacaatac ccggcagata aacaacatat ccggcacgct taccttccgg cacgctttgt
    40801 tcaggaaacg cttccggcac cacgcgtacc cgacgccgcg aacgggcgta aatgacccaa
    40861 ccgccaatgc caatagccac taccggaagc acccacagca gcacggtcag cggcgttaac
    40921 ggcggatcgt aagtgacgaa gttgccgtaa cgcgccacca tataatcgac aatctctttt
    40981 ttacttttac cttcctgcat cagttcatac actttctggc gcaggtcggt ggcaatcatc
    41041 gagttggaat cggcaatgct gttgttctgg catttcgggc agcgcagttc ttcagtgagc
    41101 tgacggaact gttgttcctg tgcttcatct ttaaactgca acacgtcgat ggtcgccagc
    41161 gctgagccgg agatcatcag catcagcacg cccaataaaa acctcattgt gcggcctcct
    41221 tactgtattt ctcccacagc ggcttgatct cttcttccca gacgcgagga ttcagatcgc
    41281 ccgcatggcg atagcgaatg atgccgttgc cgtcaataag gaacgtttct ggcgcgccat
    41341 agacaccgag atccagccct aacatgccgt cgccatcaaa caggcttagc gcgtaaggat
    41401 tgcccagctc tttcagccag ctgattgcct tctggcgatc gtctttatag ttcatgccga
    41461 ccacgcgaat gccctgcgct gaaagctgat tcagatattg atgttccgca cggcaggtcg
    41521 gacaccaggt cgcccagacg ttaagcagta ctggtttgcc ctgagtcagc acatccgcct
    41581 gataaaactg ccccggattg tccagtgatt cgagacgaaa cttcggcaca ggcttgccaa
    41641 tgagcgccga ttccagattg gtcggatcat ccccttcggc attacgcgcc agctgccaca
    41701 gcagcgccgc ggcaatcgcc aggaagataa tcaacggaat taacaatact ttgcgcttca
    41761 tacggcctcc ggcgcagttt tttgcggact cacgcgctta cgatagcgag gatcaaacag
    41821 acacagtagt ccgcccaacg ccatcatcag cccgcccgcc caaatccagc gaacaaatgg
    41881 tttgtagtaa agacgcacgg cccacgcgcc gttttccagc tcttcaccga gcgcggcgta
    41941 caggtcacgc gtgatgccgc cgtcaattgc cgcttcggtc atcatcgacc cggcagtgtt
    42001 gtaataacgt ttttccgcat acagcaccgt ttccggttta ccctctcgcg ttacgccaat
    42061 agtcgccaca ccgccgcgcc agttcgggcc agtcacctct ttgacatcac ggaaggtgaa
    42121 gcgatattca tgaatatcga cgctatcgcc ggacttcatg cgcacatcac gctcaacgct
    42181 ataattctgg ctaaaggcaa tgccaacaat tgtcactgcc agccccaggt gagccgccac
    42241 catcccccaa taactgaagg tggttttcgt gccgcgtgaa atacgtagcg cagcttccgc
    42301 aattgccagc accgcaatcc agcaggccat tgccaggccg agcaccgtca tcgccaccac
    42361 tttgctttcg aacagccacg gcaacagcag agacagcacc agcgtagaga tgaaggcgat
    42421 aatcaataaa ttgcggattt tacgtgggcg atcgcgcccc cagcgcacca gaggaccgac
    42481 acccagcagt agcgcaaacg gcaccatcag ccaggtaaac atggtgttga agaacggttc
    42541 gccaatcgaa atactgccca gtcccagttg cttgtgcacc agcggcagca gcgtccccag
    42601 caacaccacc agcatcgcgg cgaccagcaa aacgttgttc gctaacagca gagattcccg
    42661 cgaccacagc gcattgttta cgcgtgagcg aactttgtgt ccacgcgcgg caaacagcag
    42721 cagcgaaccg ccaatcacca gcaccataaa ggcgaggata aacataccgc gcgccggatc
    42781 agacgcaaac gcgtgtaccg ataccagcac gccggaacgc acgaggaaag tccccaacag
    42841 acacaacgag aaggcactga ttgccagcaa taatgtccac gctttgaagc tggcgcgttg
    42901 ttcagtgacc gccagtgagt gcatcagcgc agtccccacc agccacggca taaacgaggc
    42961 gttttctacc ggatcccaga accaccagcc accccagccg agttcgtaat aggcccatgc
    43021 ggaaccgagc acaatgccga gcgtcaggaa aatccacgcc gccagcgtcc acgggcgagt
    43081 aaaacgcgca taagtgctgt ccagacgccc gctcagcaaa gaagcaatgg caaaagcaaa
    43141 cgccaccgag aaacccacgt accccatata gagcagaggc ggatggaaga tcagccccgg
    43201 atcctgtaac agcgggttaa gatcgcgccc ttcaatcggg aagttcggca gcgtgcgtga
    43261 gaatgggtta gaggtaaaga gaatgaacag caaaaagccg acgctgacca tccccattat
    43321 cgccagtacg cgggccacaa tatccagcgg aatacgctga ctaaaaatcg ccaccgcaaa
    43381 ggtccagccg ctcatcagca gcacccacag cagcagtgag ccttcatgtg cgccccaggt
    43441 cgccgccacg cgataccaca ccggaagctg ggtattggag ttgctggcaa cataggtgac
    43501 ggtaaagtcg ttgaccacga aagcattgac cagcaccaga aatgcgccag ccacagacat
    43561 aaacagcagc caggcaaaca agcgggaaga cgccatcatg cgcgcatctc cgcgcgccac
    43621 gccccatagc ggatacacgg acagcagcag cgcaatccct aacgccaggc acagcagccc
    43681 gtttccaatt tccggcatca tgatgctggg tccttataaa cactcgccgg gcgacggtgg
    43741 ttagcttcca tcgctttctc aacttctggc ggcgtgtagt tttcgtcgtg tttcgccagc
    43801 acttcttttg cgaggatatg attgcctttt tccagctcgc cctgcaccac aacgccctgc
    43861 ccttcacgga acagatccgg caaaatgcct tcgtaagaga catccactga gccttcagcg
    43921 tcgtaaatgg tgaaggtcac tttcagcgaa ttgggatcgc gctgcacact acccggcatc
    43981 accatcccgc caacgcgcag acgctgaccg acttccggca tttgctgagt ttcacgcttg
    44041 ccgtagagaa tttcccccgg cgtataaaag agatcgatat tcgagcgcag cgcatatagc
    44101 accagaccga tagtcagcgc cagccctgcc aacacggcac aggcaatcca caagcggttt
    44161 ttacggcgaa tattcatgca gcctcctgct gttgcgcagc acgtaaacgc gcctcacgcg
    44221 cccgctgttg cgccacgcca cgcagaattg cgcgatgttg catcaccgag tgcacgacca
    44281 aaaccaccag cggaataacg gtcatcacca ccgccagcca gacaaaaaag gcgtaaccgc
    44341 ccattgcgaa aaattcattc caggaagcaa atgcaggggt catttacggc ctcttttcag
    44401 tatcagttca ctcacccacg gacggcgttt ttccatcagc aaaatcaaat tacgcatccg
    44461 catcagcgtc agcgtggcag acagtagcag gaagccaaaa atcgaccagc gcagcggcga
    44521 acgcatcgcc ggatcgatac tttgctgcat ccgcgttgat ccctgatgca gggtgttcca
    44581 ccactccacg gagtaatgaa taatcggcag attcaccacg ccaatcagca ccagaatacc
    44641 tgccgcacgg cccgccagac ggcggtcgtc gaaggcgtgc cacagggcaa tcacacccac
    44701 atacaaaaac agcagcacca gttcagaagt cagacgtgca tcccataccc accaggtgcc
    44761 ccacatcggt tttccccatg cagagccggt aaccagggca ataaaggtaa acacggcacc
    44821 aatgggggcc atcgccgcca ccgccaggtt ggccattttc atctgccaga caaggccaat
    44881 aaacgccgcc accgccattg atgcataaat gcccatcgac cagatcgccg caggcacatg
    44941 caggtagata atgcggtagc tatttccctg ctgataatca gccggagcaa agccgaatcc
    45001 ccagatccag ccgacggtaa gcacgaccac actggcaatt gccagccacg gtataaacca
    45061 gccacagatt tgatacagcc gtggtgggat cgccagttga tgcagtgttt tccacatagt
    45121 ttcgatacca gactcgaaca aaaatcagta atccagcgtt attgaatgct gattcgtaac
    45181 gctgccgccg tcgcaaaagg acttaatgtc gcggtgcctg ccagcaacgc gcctaaaatt
    45241 gccagatacc cgtcaacggg caaatgcatg gaagccgcgt ccatcgcggc ggtggcaaag
    45301 atgagtaatg ggatagtcag cggtaacacc agtatgctga gcagcacacc accgcgctta
    45361 agtcccactg tcaacgccac acccggtgca ccgagaaagc caagcgtagg cgttcccagc
    45421 agcagcgtca gcgccatcac ttgccagcca taaacatcca ttcccagtag cattgctacc
    45481 agtggcgaaa ggatgagtaa cggcagaccg gttaccatcc agtgcgccat caccttcgcc
    45541 agcacaacgg cgggcaaggg taacggcaac aacatcaatt gttcaagact gccgtcctgc
    45601 aaatcgtcac ggaacagtcg ttccagcgcc agcaaggatg aaagcagcgc agcaacccag
    45661 ataatgcccg gtgcaatacg cgccagcagt tgcggctccg gaccgatact gagcggaaaa
    45721 agggtaatta caatcaggaa gaaccacagc gggttggcga tttcggcgct atggcgaaac
    45781 gctacacgca gctcaagacg gaaaatgcgc cagaacatca tgcggccctc gtttgcgtca
    45841 gtgaaatgcg gcgaatttta ctttcagcaa cgttgagcgg ctggtgggta gtcagaatca
    45901 caatcccccc ctgctccgta tgctgcgcca tacgctgggt cagacgatcg acgccgttaa
    45961 cgtcaatcgc ggtaaaaggc tcgtcgagga tccataacgt ggcacgggtc agccacagac
    46021 gcgctaaagc gacgcggcgt tgttgcccgg ccgagagctg atttacagga atatcttcga
    46081 atccggcaag cccggcctgc gccagggctt ccagacattg tgcggtatcg ccatcgcgat
    46141 gataaaagtg cagattttct aacgccgtca gccgggtttt gatccccggc tgatggccta
    46201 tccataacag gttttgatgg tagctgtcgc gtacctgatg caagggctgc ccttgccaga
    46261 gaacctcgcc tgcgtcaggg cgagacaacc ccgtcagcaa acggagaagc gttgtcttcc
    46321 ccgcgccgtt gctaccggtg atttgtaccc actctcctgc gttcagcgta aatgacaagc
    46381 cactaaataa ggttcgttca tcccgctcac aaagtaactc tctggcttca agcataccca
    46441 cgcaataacc ctgttaaaaa cctggctcga cttcacgcat atcgggcagc ttgtgcgcta
    46501 tccctttatg gcaatcaata caggtttgcc catctttcac cgcctggtca tgcatcttcg
    46561 cggcaaccga tttctgggcg gttgtatcca tatactcgaa gttgtgacag ttacggcact
    46621 cctgcgagtt attgtccttc atgcgccgcc actcattctg tgccatcgtc agacgatgag
    46681 cttcaaattt ctgcggcgtg tcaataacgc caaaaatttt accatacagc tctttacttg
    46741 ctttgagctt gcgtatcatc ttcggcacaa actcgtgcgg aacgtgacaa tccggacagg
    46801 tcgcacggac gccgctacgg ttgttgtagt gcacggaatc catgtattcc tgatacaccg
    46861 tgttgcgcat ttcgtggcag ctaatgcaga actcttcggt attggctttt tccatcccgg
    46921 tgttaaagcc accccagaag acgatgccgc caacaaaacc gatcaacagc agcgtcccca
    46981 gcgccagacg gctgggggta cgccaccatt tccacaggcg cttaatcaga ccaggcttac
    47041 ggtcagaatt tcccataata acctcttatt tcccgtaacc ttttgatggg gtaaaggtat
    47101 tccccacgat tggcgcggta tcggcctgcg gtacgtgaca ttgcagacag aaataacgac
    47161 gcggagccac ttccgcgccc actttgccgt cgctgtccat aaagtgagta ggactgatac
    47221 gcggcgcgcc agtggtgcga tagctttcga caccgtggca ctgcaagcag cggttggtat
    47281 tggtcgttac ctgataacct tcaacgctat gcgggatcat tggcggctga ttcacatagt
    47341 tcagcggcat ccgttcctgc tcttttggca tccgaatggc cccttcctgt gtcccggata
    47401 cttccggcga ctggctaaaa tcgacgccat tagccgccca aaccgcgccg cttaccacca
    47461 aggccagcat cgccgtccat tgacacagcg ctttcttcag gtcatggctt ttcatgattt
    47521 cgctcccgaa ctccatcgtg tagttattgt aaatacatcc tcagaacaga catccacgca
    47581 gcgaccgcac gtcatgcaat cgcggctggt gacctgcacc gggctttgtt catccagcac
    47641 cggggcacgt agcacatgcg gttccgggca aacatgaaaa caatccatac agcggttaca
    47701 tttctgccga tcggtggccg caacggtaat cacacctttg ctacccagca cgccatacag
    47761 cgcgccaacc ggacaaatgt gcccgcacca gccgtgttca acgaccagta gatcaaataa
    47821 aaacagcgcg agaataagca gcgcgccgct gccgaagccc attaccaggc tacgccccat
    47881 cagagaaacg gggttgatcc attcccaaat gagcgtgcca gttaaggctg atcccaccag
    47941 aatgaccacc aacagcacgt agcgaatgtg gcgagggatc gtcgcagact gattgaggtc
    48001 aaagcgcctg cgtaaccagt tcgctaagtc ggtaatcggg ttcagcgggc agacccagct
    48061 gcaaaataat cgctttcccg ccagggcgta gagcacggtg ataatcaccg cgccggtcaa
    48121 cgccaccgtg gcaggcagat gaccactggc aagactttgc agcgtcatca gcggatcggt
    48181 cagcggaacg gtgtcgaata acaggctact gctgtagttg ccgtgcaaga tccacacacc
    48241 aaaccatgga ccgctcaaaa acatccccag cacgaagaac tgacaaaggc gacgtaacac
    48301 cagccaacgg tgactgcgcc accagccttt tttctccagc gcctcgcgcc cggcgtcacg
    48361 tttacgattt gccattgttc ccctccagcc agccgaagcg gtaatggtga cctaactccc
    48421 ctttcgccag tgacagcggt aacaccttga ttgccggttg ttccagcacg catacttttt
    48481 cgcacttacc gcaaccggta caggcgtcgc tgtgaaccgt cggcagaaag cgggcgtgct
    48541 taccggtacg cgtgttgcgc tccagctcca gggtgatggc ctcatcaatt ttcgggcatt
    48601 cgcgataaca aacatcgcag cgcagcccct gaaagttgag acagttttcc tggtccacca
    48661 gtaccgccag ccccatccgc gcgtcgtcga tcgattcaat ctcacgatcc agcgcaccgc
    48721 ttgggcacac tttggcgcac ggaatgtcct cacacatttc gcaaggaata tcccgtgcga
    48781 caaaatatgg cgtgcccgcc gacagaccag aggccagcgt cgccagtttt aaggtgtcgt
    48841 aagggcaagc ctgaacacac tgaccgcaac gcacacaggc actggcaaag gcgttctcgt
    48901 ttatggctcc gggcgggcgc aaccgcacgc cagatgcgcg tgcggtttgc tgttgtaacc
    48961 ccagcgccac acccacggca gccagcccgc ctgctgtgcg aacaacatcg cgcagaaagc
    49021 ggcggcgacc attttgaggt ttcgctgacc gggacataat ggcttacacc ttctccagtt
    49081 tgaccgcgca cttcttgaag tccgtctctt tcgagagcgg atcggtcgca tccagcgtca
    49141 gtttgttaac cagctgtgcg gcgtcgaaga acggcatgta caccaggccc tgtggcggac
    49201 ggttacgacc gcgcgtttca acaatcgaga tcacttcgcc acggcgagaa accactttca
    49261 ctttgtcgcc acggcgcaga tcgcgcgctt tcgcatccag cgggtgaata aacaagaccg
    49321 cttccgggaa ggcgcggtgc agttccggca cacggcgagt catactgccg gtgtgccagt
    49381 gctccaggac gcgtccggta gagagccaca ggtcgtactc ttcatccggt gcttccgccg
    49441 ccggttcgaa cggcagcgcg aagatcaccg ctttgccatc cggtttaccg tagaacttgt
    49501 agccttcgcc cgctttcacg tacgggtcgt taccttcgct gtaacgccac tgcgtttctt
    49561 taccgttcac caccggccag cgcagaccgc gcgctttgtg gtagtcatcg aacggtgcca
    49621 gatcgtgacc gtgaccgcga ccaaaccatg cgtactcttc gaacagccct ttttgcagat
    49681 agaagcccag ctcgcgggat tcatcgttca gctgatcttc cgccagttcg gataccggga
    49741 atttgctcac ttcgggggtg gcatacagaa cttcgtacag cgttttgcca cgcagttccg
    49801 gtttcttcgc cagcagctct tccggccata cttcttcagt tttgaagcgg cgggagaact
    49861 ggactaactg ccagagatcc gatttcgctt cgcccggtgc ctgtacctgc tgacgccaga
    49921 actgagtacg gcgttcggcg ttaccgtaag cgccctcttt ctctacccac attgcggtcg
    49981 gcaggatcaa gtcggcggcc agcgcactga ctgtcggata cggatcggag acgatgatga
    50041 agttgcgcgg atcgcgccag cccggcatac gctcttcatt gatgttcggc ccggcctgca
    50101 tgttgttggt acacatggtc cagtaaacat tcagcttgcc gtctttcagc gcacggtctt
    50161 gtgctaccgc atgcagaccg attttcgccg gaatggtgcc gctcgggata ttccacttct
    50221 tctcgcagat atcacgatgt ttctcgttag tcaccaccat gtccgcaggc agacggtgag
    50281 caaaggtgcc cacttcacgc gcagtaccac acgcggaagg ctgcccggtc agggagaacg
    50341 gaccgcaacc cggctgggaa attttgccgg tcagcaggtg caggttgtag accaggttgt
    50401 tggcccacac gccacgagta tgctggttga agcccatcgt ccagtaggag atgactttct
    50461 tgttcggatc ggcatacagc tgcgccagtt gttctaactg gtctttcggc acgccggtca
    50521 tttcggcagt tttttccagc gtatactcgg caacgaaggc tttgtaatct tcaaagctca
    50581 tcggttcgga ggcgtcagaa cccggattct tcgctgcttt ttccagcgga tgggtcggac
    50641 gtaaaccgta gccgatgtcc gtcgcccctt tgcgcaggtt aacgtgcttg ctgaagaagt
    50701 cctgatttat cgcattgttt tgaatgatat agttggcgat gtagttcagg atcaccaggt
    50761 cagattgcgg cgtaaagatg atgccgttat ccgccagctc gaagctacga tgctggtagg
    50821 tagaaagcac cgccacggtg acgttctggt tagagagacg acggttagtg atgcgtgacc
    50881 agaggatcgg gtgcatctcc gccatgttag cgccccacag cacaaacgcg tcagcctgct
    50941 cgatgtcgtc atagcagccc atcggctcat ccataccaaa ggtacgcata aagccaacta
    51001 ctgccgacgc catacagtga cgtgcgttcg ggtcgatgtt gttcgaacgg aagcccgctt
    51061 tgaacagctt ggacgcggca taaccttccc agatagtcca ctgaccagaa ccgaacatac
    51121 cgatcgattc cggtcctttt tctttcaggg cggttttgaa tttctcttcc atcacatcga
    51181 aggcctgatc ccaggtgatt ggggtaaatt cgccttcttt gtcatattta ccgtttttca
    51241 tacgcagcag cggctgcgtc aaacggtctt taccgtacat gatcttgggc aggaaatagc
    51301 ccttaatgca gttcaggcca cggttaaccg gtgcgtccgg gtcgccctga caggccacca
    51361 cacgcccctg ctgcgttccg accagaacgc cgcaaccagt accgcagaaa cggcacggcg
    51421 ctttatccca tttaatggct tcctgctgac caacaacggc gcgggcaacg cccggcacgc
    51481 tgagaccggc agccgccgca gcggccgcaa cggcgttagc tttcataaag ctacgacgac
    51541 tgagtttcat ggtgtttcct caccttgctc ttcctgctgg tgataaacca gcgacaccgc
    51601 cagcacgccc tctacgttgc gtactgactc aatggtttgg atcagcgttt cgctgtcttc
    51661 tgcttccacc accacaatca actgaccgct cggcgcgtcg ctgacagcaa cttcacagcc
    51721 gggaaaggcg ttcagttggg tgctgatgtc tgaaattcgt tcgcttttgg cctgcacgac
    51781 caggctgcaa acttgccagt tagtgtgcat ggagatactc cgcagttatg gctgataccg
    51841 gacagctggc tgcacatgct ccacatccgt tgcaaagttg gctattaagt tgcggctggt
    51901 agattccgga gagcgtcggg cgaaagataa ttgccattgg ctcacagcta tcctgacagc
    51961 ggcggcattc aactgactga tacgccaggc acgcgtcccc aatggtgaac tgcaaatccc
    52021 aggccctggt gtggcgcgga gaaaatagcg attcggggca agcctgcgcg caggcgtaac
    52081 agaagctgca ctcgttattt ttgaaattaa cgctcggata gccgcccgcg ccgcgttgca
    52141 gaatgttgtt ttcacaagca ttaatacagg cgtcgcaacg ggtgcaatgg gtcagaaaat
    52201 gagattcatc accggaccag ggcggacgga taccgttact ggctttgcgc cagcgaccag
    52261 tgagtatgcc ccgacgggat gcatcaatct tcacattgac cttccatcat taacgcgctc
    52321 tgaaaattga gagcgaccaa ataaaccgca taattaataa gccattttta tagccgctaa
    52381 gatattaaag gatgtgtcaa agatgcatac cccgatcggg gtaaaacctg tagcaggatc
    52441 aaaagagtgg cgggaagcgt ggcaaaaacg ggcttttgct cacatttcaa atggttataa
    52501 acatatttat atagcgatta attcaccaga gatatttctg ctggtttgct ccctcatcag
    52561 aatttaacac taaaagagca ggtaaaattg tctgaatgtt ctttaagtta ttcataaagc
    52621 aaattaataa atctgatgaa tatgttaacc ttcagcgaca tcatcggtga aaacctataa
    52681 atgaagaagg aaagcaaaaa aatgaagacc attctacctg cagtattgtt tgccgctttc
    52741 gctaccactt ccgcctgggc ggcagaaagc gtccagccac tggaaaaaat cgcgccttat
    52801 ccacaagctg aaaaagggat gaagcgtcag gtgattcagt taaccccgca agaagatgaa
    52861 tctaccctga aagtagaact gttaatcggt cagacgctgg aagtcgattg caatttgcat
    52921 cgtctcggcg ggaagctgga aagcaaaacg ctggaaggct ggggctatga ttattatgtc
    52981 tttgataaag ttagctcgcc ggtttccacg atgatggcct gcccggatgg caagaaagag
    53041 aagaaatttg tcaccgcgta tctgggcgat gctggaatgc tgcgttacaa cagcaagctg
    53101 ccgatcgtgg tgtatacgcc agacaatgta gatgtgaagt accgcgtctg gaaggcggaa
    53161 gagaaaattg acaacgcggt agttcgctaa actgccgtga agtgcggcac cccgtaggtc
    53221 agacaaggcg gtcacgccgc atccgacatc caacgcccga gccggttgcc tgatgcgacg
    53281 ctggcgcgtc ttatcaggcc tacaccgctg tgaagtgctc caccccgtag gtcggataag
    53341 gcggttacgc cgcatccgac atctaacgcc caagccggtt gcctgatgcg acgctggcgc
    53401 gtcttatcag gcctacaccg ctgtgaagtg ctccaccccg taggttggat aaggcggtta
    53461 cgccgcatcc gacatctaac gcccaagccg gttgcctgat gcgacgctgg cgcgtcttat
    53521 caggcctaca ccgctgtgaa gtgctgcacc ccgtagattg gataaggcgg ttacgccgca
    53581 tccgacatct aacgcccgag ccggttgcct gataagacgc tggcgcgtct tatcagcata
    53641 cgccacatcc ccggcatacc atgccggatg tggcacatca ttacaacgca atatccgcca
    53701 cttctttttg aacgggtttc ggtttcaact gcggtttcgg cgtactatct gctgcctgcg
    53761 gcttgtcgta tttcagcccc agcacttcgc tggtgtactg caactcgcgt tctgttgccg
    53821 ctacatcacc gttcagcttg cgtccataag acggaacgat cgctttcaac gtagcctgcc
    53881 attgcgggct ggaaacgcga tcgccaaata ctttttccag cagatccaac ataatcggcg
    53941 cggcggttga cgcccctggc gatgccccca ggagcgcggc aatggtccct tgctggtcac
    54001 tgacgacttc agtacccaga cgcagtacgc cacctttctc ggcatcacgc ttgataatct
    54061 gcacgcgctg ccccgcttgc cacaaacgcc agtcctcttt tttcgcctgc ggatagtact
    54121 ctttcaacgc ttcaaaacga tcctcttcac tcaacatcac ctgactcacc agatatttca
    54181 ccagatcgaa attatccagc ccgacgtgca tcatcggcat cacgttagag gtggtggtgg
    54241 aactcatcag atcccacaat gaaccgtttt tgaggaattt ggttgagaag gtggcaaatg
    54301 gcccaaacag cactacgcgt ttaccgtcca gaacgcgggt atcgatatgc ggaaccgaca
    54361 tcggtggtgc gccaacggat gctttaccgt aaaccttcgc cagatggtga ttaaccacgt
    54421 ccgggttttc cgaaacaagg aactgtccgc ccaccgggaa accggcgtag tctttcgctt
    54481 ccggaatccc cgattcctgt aacagcttca gcgccgcacc gcccgcgccg ataaagacaa
    54541 atttggcacg aatgttctgt gcagtgccat ttttcagatc ggcaacggta acggtccagg
    54601 tgttatcgtc attacgcttt agggcgcgga cttcgctgct gagttgcagc gagaagttag
    54661 atttcttctg caaggaagca attaactggc gggtgatctc gccgtagttc acatcggtac
    54721 caatttccgt acgcgtggct gccactttct gttgcggatc gcgcccttcc atcactaacg
    54781 gtgcccactc tttgatctgc gcgtgatctt cagagtaacg cataccgcga aacagcgagc
    54841 tttgttgcaa cgcggcgtaa cgggcgcgca ggaaattaac gttatcctcg ccccagacaa
    54901 agctcatatg cggaacggta ttgataaatg aacgcggagt acgcagcacg ccgcgctcaa
    54961 cctggtgcgc ccagaactgg cgggaaatct gaaatgcttc gttaatggcg actgcttttt
    55021 caatactgat gctgccatcg gcgttttgcg gggtgtagtt cagttccatc agtgcagaat
    55081 gcccggttcc ggcgttattc cagccgttcg aactctcctg cgcgacaccc tccaggcgct
    55141 ccaccatggt catcgaccat tcaggctcca gctcgcgtaa ataggtcccc aacgtggcgc
    55201 tcataatgcc gccgccaatc aacagtacat cagtttcctg ctcctcagac gctttcgctt
    55261 ttgccgccat cgaaacggca ttaagcccca cggccatcga gaagagcatg gcagtcactt
    55321 ttttcatctt gttaatgcct tacttttagt cgctttattg caggtgagat ttgcgcggca
    55381 tcaacggtaa cacttaagta acaacattta aataatgttt
//
LOCUS       DBANUU010000009        54582 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000009 DBANUU010000000
VERSION     DBANUU010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.009"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..144)
                     /locus_tag="ACIE68_000536"
     CDS             complement(<1..144)
                     /locus_tag="ACIE68_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFX5906904.1"
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTEL
                     GRNL"
     repeat_region   259..1081
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=260..288
                     /rpt_unit_seq="gtgttccccgcgccagcggggataaaccg"
     gene            complement(1102..2139)
                     /gene="iap"
                     /locus_tag="ACIE68_000537"
     CDS             complement(1102..2139)
                     /gene="iap"
                     /locus_tag="ACIE68_000537"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFX5906905.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            2391..3299
                     /gene="cysD"
                     /locus_tag="ACIE68_000538"
     CDS             2391..3299
                     /gene="cysD"
                     /locus_tag="ACIE68_000538"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFX5906906.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            3301..4728
                     /gene="cysN"
                     /locus_tag="ACIE68_000539"
     CDS             3301..4728
                     /gene="cysN"
                     /locus_tag="ACIE68_000539"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFX5906907.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEKTFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMAWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFINRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            4728..5333
                     /gene="cysC"
                     /locus_tag="ACIE68_000540"
     CDS             4728..5333
                     /gene="cysC"
                     /locus_tag="ACIE68_000540"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFX5906908.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            5383..5706
                     /gene="ygbE"
                     /locus_tag="ACIE68_000541"
     CDS             5383..5706
                     /gene="ygbE"
                     /locus_tag="ACIE68_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFX5906909.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            5900..6211
                     /gene="ftsB"
                     /locus_tag="ACIE68_000542"
     CDS             5900..6211
                     /gene="ftsB"
                     /locus_tag="ACIE68_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFX5906910.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            6230..6940
                     /gene="ispD"
                     /locus_tag="ACIE68_000543"
     CDS             6230..6940
                     /gene="ispD"
                     /locus_tag="ACIE68_000543"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFX5906911.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            6940..7419
                     /gene="ispF"
                     /locus_tag="ACIE68_000544"
     CDS             6940..7419
                     /gene="ispF"
                     /locus_tag="ACIE68_000544"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFX5906912.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            7416..8465
                     /gene="truD"
                     /locus_tag="ACIE68_000545"
     CDS             7416..8465
                     /gene="truD"
                     /locus_tag="ACIE68_000545"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFX5906913.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            8446..9207
                     /gene="surE"
                     /locus_tag="ACIE68_000546"
     CDS             8446..9207
                     /gene="surE"
                     /locus_tag="ACIE68_000546"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFX5906914.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            9201..9827
                     /gene="pcm"
                     /locus_tag="ACIE68_000547"
     CDS             9201..9827
                     /gene="pcm"
                     /locus_tag="ACIE68_000547"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFX5906915.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            10012..11106
                     /gene="nlpD"
                     /locus_tag="ACIE68_000548"
     CDS             10012..11106
                     /gene="nlpD"
                     /locus_tag="ACIE68_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFX5906916.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            11169..12161
                     /gene="rpoS"
                     /locus_tag="ACIE68_000549"
     CDS             11169..12161
                     /gene="rpoS"
                     /locus_tag="ACIE68_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFX5906917.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(12255..13619)
                     /gene="ygbN"
                     /locus_tag="ACIE68_000550"
     CDS             complement(12255..13619)
                     /gene="ygbN"
                     /locus_tag="ACIE68_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFX5906918.1"
                     /translation="MSTITLLCIALTGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(13708..14484)
                     /gene="ygbM"
                     /locus_tag="ACIE68_000551"
     CDS             complement(13708..14484)
                     /gene="ygbM"
                     /locus_tag="ACIE68_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFX5906919.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            complement(14489..15127)
                     /gene="ygbL"
                     /locus_tag="ACIE68_000552"
     CDS             complement(14489..15127)
                     /gene="ygbL"
                     /locus_tag="ACIE68_000552"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFX5906920.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(15124..16386)
                     /gene="otnK"
                     /locus_tag="ACIE68_000553"
     CDS             complement(15124..16386)
                     /gene="otnK"
                     /locus_tag="ACIE68_000553"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFX5906921.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(16383..17291)
                     /gene="ltnD"
                     /locus_tag="ACIE68_000554"
     CDS             complement(16383..17291)
                     /gene="ltnD"
                     /locus_tag="ACIE68_000554"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFX5906922.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            17487..18254
                     /gene="ygbI"
                     /locus_tag="ACIE68_000555"
     CDS             17487..18254
                     /gene="ygbI"
                     /locus_tag="ACIE68_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFX5906923.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(18305..18961)
                     /gene="pphB"
                     /locus_tag="ACIE68_000556"
     CDS             complement(18305..18961)
                     /gene="pphB"
                     /locus_tag="ACIE68_000556"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFX5906924.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFYPETD
                     LLISTGDNIDRGPKSLNVLRLLNQPWFTSVKGNHEAMALDAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(19067..21628)
                     /gene="mutS"
                     /locus_tag="ACIE68_000557"
     CDS             complement(19067..21628)
                     /gene="mutS"
                     /locus_tag="ACIE68_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFX5906925.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            21704..21838
                     /locus_tag="ACIE68_000558"
     CDS             21704..21838
                     /locus_tag="ACIE68_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5906926.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            21915..22268
                     /gene="ygbA"
                     /locus_tag="ACIE68_000559"
     CDS             21915..22268
                     /gene="ygbA"
                     /locus_tag="ACIE68_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFX5906927.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKTRE"
     gene            complement(22305..24383)
                     /gene="flhA"
                     /locus_tag="ACIE68_000560"
     CDS             complement(22305..24383)
                     /gene="flhA"
                     /locus_tag="ACIE68_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFX5906928.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(24457..25467)
                     /gene="hypE"
                     /locus_tag="ACIE68_000561"
     CDS             complement(24457..25467)
                     /gene="hypE"
                     /locus_tag="ACIE68_000561"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFX5906929.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAVDKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(25464..26585)
                     /gene="hypD"
                     /locus_tag="ACIE68_000562"
     CDS             complement(25464..26585)
                     /gene="hypD"
                     /locus_tag="ACIE68_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFX5906930.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTNAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            complement(26585..26857)
                     /gene="hypC"
                     /locus_tag="ACIE68_000563"
     CDS             complement(26585..26857)
                     /gene="hypC"
                     /locus_tag="ACIE68_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFX5906931.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(26848..27720)
                     /gene="hypB"
                     /locus_tag="ACIE68_000564"
     CDS             complement(26848..27720)
                     /gene="hypB"
                     /locus_tag="ACIE68_000564"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFX5906932.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIAEAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(27724..28074)
                     /gene="hypA"
                     /locus_tag="ACIE68_000565"
     CDS             complement(27724..28074)
                     /gene="hypA"
                     /locus_tag="ACIE68_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFX5906933.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            28286..28747
                     /gene="hycA"
                     /locus_tag="ACIE68_000566"
     CDS             28286..28747
                     /gene="hycA"
                     /locus_tag="ACIE68_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFX5906934.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            28872..29483
                     /gene="hycB"
                     /locus_tag="ACIE68_000567"
     CDS             28872..29483
                     /gene="hycB"
                     /locus_tag="ACIE68_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFX5906935.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            29480..31306
                     /gene="hycC"
                     /locus_tag="ACIE68_000568"
     CDS             29480..31306
                     /gene="hycC"
                     /locus_tag="ACIE68_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFX5906936.1"
                     /translation="MSAISLINSGVAWFVATAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQALAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            31309..32232
                     /gene="hycD"
                     /locus_tag="ACIE68_000569"
     CDS             31309..32232
                     /gene="hycD"
                     /locus_tag="ACIE68_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFX5906937.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            32250..33959
                     /gene="hycE"
                     /locus_tag="ACIE68_000570"
     CDS             32250..33959
                     /gene="hycE"
                     /locus_tag="ACIE68_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFX5906938.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            33969..34511
                     /gene="hycF"
                     /locus_tag="ACIE68_000571"
     CDS             33969..34511
                     /gene="hycF"
                     /locus_tag="ACIE68_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFX5906939.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            34511..35278
                     /gene="hycG"
                     /locus_tag="ACIE68_000572"
     CDS             34511..35278
                     /gene="hycG"
                     /locus_tag="ACIE68_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFX5906940.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            35275..35685
                     /gene="hycH"
                     /locus_tag="ACIE68_000573"
     CDS             35275..35685
                     /gene="hycH"
                     /locus_tag="ACIE68_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFX5906941.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            35678..36148
                     /gene="hycI"
                     /locus_tag="ACIE68_000574"
     CDS             35678..36148
                     /gene="hycI"
                     /locus_tag="ACIE68_000574"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFX5906942.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            36173..36934
                     /locus_tag="ACIE68_000575"
     CDS             36173..36934
                     /locus_tag="ACIE68_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5906943.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            37108..37419
                     /locus_tag="ACIE68_000576"
     CDS             37108..37419
                     /locus_tag="ACIE68_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFX5906944.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            37416..37835
                     /locus_tag="ACIE68_000577"
     CDS             37416..37835
                     /locus_tag="ACIE68_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFX5906945.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(37949..39373)
                     /gene="ascB"
                     /locus_tag="ACIE68_000578"
     CDS             complement(37949..39373)
                     /gene="ascB"
                     /locus_tag="ACIE68_000578"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFX5906946.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(39382..40839)
                     /gene="ascF"
                     /locus_tag="ACIE68_000579"
     CDS             complement(39382..40839)
                     /gene="ascF"
                     /locus_tag="ACIE68_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFX5906947.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVMLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            41099..42109
                     /gene="ascG"
                     /locus_tag="ACIE68_000580"
     CDS             41099..42109
                     /gene="ascG"
                     /locus_tag="ACIE68_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFX5906948.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            42258..42785
                     /gene="hydN"
                     /locus_tag="ACIE68_000581"
     CDS             42258..42785
                     /gene="hydN"
                     /locus_tag="ACIE68_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFX5906949.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            42938..45190
                     /gene="hypF"
                     /locus_tag="ACIE68_000582"
     CDS             42938..45190
                     /gene="hypF"
                     /locus_tag="ACIE68_000582"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFX5906950.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LADKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(45318..46451)
                     /gene="norW"
                     /locus_tag="ACIE68_000583"
     CDS             complement(45318..46451)
                     /gene="norW"
                     /locus_tag="ACIE68_000583"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFX5906951.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(46448..47887)
                     /gene="norV"
                     /locus_tag="ACIE68_000584"
     CDS             complement(46448..47887)
                     /gene="norV"
                     /locus_tag="ACIE68_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFX5906952.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            48074..49588
                     /gene="norR"
                     /locus_tag="ACIE68_000585"
     CDS             48074..49588
                     /gene="norR"
                     /locus_tag="ACIE68_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFX5906953.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLSRATRNGDEVILEAQHFAFPEVTLPTPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            complement(49585..50550)
                     /gene="gutQ"
                     /locus_tag="ACIE68_000586"
     CDS             complement(49585..50550)
                     /gene="gutQ"
                     /locus_tag="ACIE68_000586"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFX5906954.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(50543..51316)
                     /gene="srlR"
                     /locus_tag="ACIE68_000587"
                     /gene_synonym="gutR"
     CDS             complement(50543..51316)
                     /gene="srlR"
                     /locus_tag="ACIE68_000587"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFX5906955.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            complement(51383..51742)
                     /gene="gutM"
                     /locus_tag="ACIE68_000588"
     CDS             complement(51383..51742)
                     /gene="gutM"
                     /locus_tag="ACIE68_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFX5906956.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            complement(51847..52626)
                     /gene="srlD"
                     /locus_tag="ACIE68_000589"
     CDS             complement(51847..52626)
                     /gene="srlD"
                     /locus_tag="ACIE68_000589"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFX5906957.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            complement(52630..53001)
                     /gene="srlB"
                     /locus_tag="ACIE68_000590"
                     /gene_synonym="gutB"
     CDS             complement(52630..53001)
                     /gene="srlB"
                     /locus_tag="ACIE68_000590"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFX5906958.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            complement(53012..53971)
                     /gene="srlE"
                     /locus_tag="ACIE68_000591"
                     /gene_synonym="gutE"
     CDS             complement(53012..53971)
                     /gene="srlE"
                     /locus_tag="ACIE68_000591"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFX5906959.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            complement(53968..54531)
                     /gene="srlA"
                     /locus_tag="ACIE68_000592"
                     /gene_synonym="gutA"
     CDS             complement(53968..54531)
                     /gene="srlA"
                     /locus_tag="ACIE68_000592"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFX5906960.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAVFEKKMGIQLEQKVHLAGATS"
BASE COUNT        12617 a        14275 c        14826 g        12864 t
ORIGIN      
        1 caggttacgg ccaagttcgg taagagtgag agttttacag tcaagtaagg cgtggcaagc
       61 caacgttaag ctgttgagtc gttttaagtg taattcgggg cagaattggt aaagagagtc
      121 gtgtaaaata tcgagttcgc acattttgtt gtctgattat tgatttttgg cgaaaccatt
      181 tgatcatatg acaagatgtg tatccacctt aacttaatga tttttaccaa aatcattagg
      241 ggattcatca gtgcttagtg tgttccccgc gccagcgggg ataaaccgtt gcccgcgcaa
      301 ttccgggagc atccgcaatt gtgttccccg cgccagcggg gataaaccga cggacaaaat
      361 atatattgat ttgcgaatta tgtgttcccc gcgccagcgg ggataaaccg gtaaagaaac
      421 tgccgacaaa tccctgttcg ttgtgttccc cgcgccagcg gggataaacc gcccgtcacc
      481 gacgcgcagt ggcgctaccg tgagtgttcc ccgcgccagc ggggataaac cgcctacgaa
      541 caattcctct gaaccgcccg cactgtgttc cccgcgccag cggggataaa ccgggatcta
      601 acgcgctgta aaaattccgt gctttgtgtt ccccgcgcca gcggggataa accgctaacg
      661 ccacattggc ggaattggct gctgatgtgt tccccgcgcc agcggggata aaccatgcgg
      721 attaccggca aaacatggga gcaaaccgtg ttccccgcgc cagcggggat aaaccggttt
      781 accgccccgc agaggcgctg gcagatccgt gttccccgcg ccagcgggga taaaccggga
      841 tgacctgtcg ctaaaactcg ccgcgtacag tgttccccgc gccagcgggg ataaaccgtc
      901 cgtttggtcc accaaatgtt tgatgcttca gtgttccccg cgccagcggg gataaaccgt
      961 tcagggtgtg attccgcata ccaagcagta agagttcccc gcgccagcgg ggataaaccg
     1021 ggcgcactgg atgcgatgat ggatatcact tagaattccc cgcccctgcg gtagaacacc
     1081 cagctcccat tttcaaaccc atcaagacgc cttcgccaac tccttcacca gaggtagcat
     1141 tatccgcata acgtcacggc agcgacgttc tattcttcca ggaagtgcct tatcaatatg
     1201 ctgttgatta tccagtctta cgtcatgcca gctatttccc gcagggaatg cggctgtttt
     1261 tgcgcgttgc tgataaccat ccttattccc aagattccag ttagtcgctt ccaccgaaag
     1321 tacagcaatg cccgctttgt cgaatatttc tgcgtcatta caacacccag tgcctttcgg
     1381 ataattttta ttcaaacccg gattggtcgt tgcggctatt ccgtgactgc gtgcaattgc
     1441 cagcgccctg tcgcgcgtta atttccttac tgcttcaggg gtttttacac cgctgttgaa
     1501 atacaattta tcgccaacaa ttaagttatc gagattaatc accagcagcg tatttttctt
     1561 ttcggtgtca ctcatccgct tgagtaaatt ctcagcgcct aatttccctt cctcttcgcc
     1621 gctggtcgcc acaaatcgaa taccatactc ggtaggcgta tttttcaggc gttctgccaa
     1681 ttccagcatg acacctaaac ctgcggcgtt atcatccatt ccttgtaacg tcagcccgcc
     1741 gagattggca tcggcgtcag catcgctcag cggggcgtaa gtatccagat gcgccataat
     1801 gatgatctgc tgcggcgctt tgccttcatg agcggcaatc accgtacttc ccgtcacgtt
     1861 atgccagctc ttacgattat cgcgggcggt ataaatatac cgactattaa atgtccgaat
     1921 atcactgcga taacccattt gctgaaactg ttggcgaata taatcggcag ataacatttc
     1981 tgcaggagtt ccggtcatgc gtcccggaaa gaaagtagca atatgtcgtg cctgagtatt
     2041 agcaaaatcg ccaggtttag gtgacgaggc gtgtacgggg agaataaagc atacgccgag
     2101 cgccagggca gcggtacggt ggcgcaatgc ggaaaacata gtgagtcctt aaataccatg
     2161 caaatttttt taccgccata gtatgaaact gccgctgcgc taaaacaatt tcaaatcttc
     2221 ctaaacgccc gaaatccggt gccttaagca ctttttgata ttagctttgc caaatcgtta
     2281 ttccgttaag gaactactca ttctaattgg taatttcatt cgttctctta cgctccctat
     2341 agtcgaaaca tctgatggca agaaaatagc ggtattgcaa aggaacggtt atggatcaaa
     2401 tacgacttac tcacctgcgg caactggagg cggaaagcat ccacattatt cgcgaggtgg
     2461 cggcagaatt ctcaaatccg gtgatgctct actctatcgg taaagattcc agcgtcatgc
     2521 tgcatctggc gcgcaaggcg ttttatccag gtacgctgcc tttcccgttg ctgcatgtcg
     2581 ataccggctg gaaattccgc gagatgtatg agttccgcga tcgtacggcg aaagcctacg
     2641 gctgcgagct gctggtgcat aaaaacccgg aaggcgtggc gatggggatt aatccattcg
     2701 tgcacggcag cgcgaaacat accgatatta tgaaaactga aggcctgaaa caggcgctga
     2761 acaaatacgg ttttgatgcc gccttcggtg gtgcgcgccg tgacgaagag aaatcccgcg
     2821 ctaaagagcg aatctactct ttccgtgacc gcttccatcg ctgggatccg aaaaatcagc
     2881 gcccagagct gtggcacaac tacaacgggc aaattaacaa aggcgaaagc atccgcgtct
     2941 tcccgctctc taactggacc gagcaggata tctggcaata catctggctg gaaaatatcg
     3001 acattgtgcc gctgtatctc gctgcggaac gtccggttct ggaacgcgac ggtatgttga
     3061 tgatgattga tgacaaccgt atcgacctgc aaccgggcga agtgattaaa aaacggatgg
     3121 tgcgtttccg tacgctgggc tgctggccgc tgaccggtgc ggtggagtca aatgcacaaa
     3181 cactaccgga gatcatcgaa gagatgctgg tttctaccac cagtgaacgt cagggccgcg
     3241 tgattgaccg cgaccaggcg gggtctatgg agctgaaaaa acgtcagggg tatttttaag
     3301 atgaacaccg cacttgcaca acaaatcgcc aatgaaggcg gcgtcgaagc ctggatgatt
     3361 gcgcaacaac ataaaagcct gctgcgtttt ctgacctgtg gtagcgtcga tgacggcaaa
     3421 agtaccctga ttggtcgcct gctgcacgat acccgccaaa tctacgaaga tcagctctca
     3481 tcgctgcata acgacagtaa acgtcacggc acccagggcg aaaagctgga tctggcactg
     3541 ctggtggacg gcctgcaagc tgagcgcgaa cagggcatca ccattgacgt ggcttaccgc
     3601 tatttctcta ccgagaagcg taaatttatt atcgccgaca ccccagggca cgagcagtac
     3661 acccgcaata tggcgactgg cgcatcgaca tgtgaactgg cgatcttact gatcgatgcc
     3721 cgtaaaggcg tgctcgatca aacccgtcgt cacagtttta tctccacact gttggggatc
     3781 aaacatctgg tcgtggcgat caacaaaatg gatctggtgg attacagtga aaagacgttc
     3841 acccgtattc gtgaagatta tctgaccttt gccgggcagc tgccgggtaa tctggatatc
     3901 cgctttgtgc cgctctctgc actggaaggc gacaacgtgg cttcgcaaag tgaaagtatg
     3961 gcgtggtaca gcggtccaac actgctcgaa gtgctggaaa ccgtggagat ccagcgagtg
     4021 gtggatgctc agccaatgcg cttcccggtg cagtacgtta atcgcccgaa tctcgatttt
     4081 cgtggttacg ccggaacgct ggcatccggt cgcgtagaag tcgggcaacg agtcaaagtg
     4141 ctgccctctg gtgtggaatc aaacgtcgcc cggatcgtga cttttgatgg cgatcgcgaa
     4201 gaagcctttg ccggagaagc gatcaccctg gtgctgacgg atgagatcga catcagccgt
     4261 ggcgatctgc tgctggcggc agacgaagcg ttaccagctg tgcagagcgc gtcggtggat
     4321 gtggtatgga tggcggaaca gccgctttcc ccgggccaga gttatgacat caaaattgcc
     4381 ggtaagaaga cgcgtgctcg tgttgatggc attcgttatc aggttgatat taataacctt
     4441 acccaacgcg aagttgaaaa cctgccgctg aacggcatcg gcctggtgga tctcactttt
     4501 gacgagccac tggtgttaga tcgttatcag caaaatccgg ttaccggtgg gctgattttt
     4561 atcaatcgcc ttagcaatgt gaccgtgggc gccggtatgg tgcacgagcc agttagccag
     4621 gcaactgctg cgccatctga atttagtgca ttcgaattgg aattgaatgc cctggttcgc
     4681 cgccactttc cacactgggg cgcgcgcgat ttgctggggg ataaataatg gcgctgcatg
     4741 acgaaaacgt cgtctggcat agccatccgg tcactgtgca acaacgcgag ctacaccacg
     4801 gtcatcgtgg tgtagtgctg tggtttaccg gcctctccgg gtccggtaaa tcaacggtcg
     4861 ccggggcgct ggaggaggcg ttacataaac tcggcgtcag tacgtatctg ctggatggcg
     4921 ataatgttcg ccacggatta tgcagcgatc tcggttttag cgatgccgat cgtaaagaga
     4981 atatccgtcg cgtcggtgaa gtggcgaatt tgatggttga agccggactg gtggtgctga
     5041 ccgcatttat ctcgccacac cgcgccgaac gccagatggt tcgcgaacgc gtaggagaag
     5101 ggcgctttat tgaagtgttt gtcgatacgc cgctggcgat ttgcgaagcc cgcgatccaa
     5161 aaggcttata taagaaagcg cgtgccggtg aactgcgcaa ctttacggga atagattccg
     5221 tttacgaagc gcctgaatcg gcagaaattc atctcaatgg tgaacaatta gtaacaaatt
     5281 tggtacagca attattagat ctgttgagac agaacgatat tatcagatcc tgagacacca
     5341 ccgggtttcc atgcccggtt cgtcaaagta acaggatcaa atatgcgtaa tagccataac
     5401 attacactaa caaataacga cagccttact gaggatgaag aaaccacatg gtcactgcct
     5461 ggtgccgtgg tcggttttat ctcctggtta tttgcgctgg cgatgccgat gttgatttat
     5521 ggctctaaca cgctgttctt ctttatctac acctggcctt tctttctggc gctgatgccc
     5581 gtcgcggtag tggtggggat tgcgctgcat tcattgatgg acggaaagct acgctacagt
     5641 attgttttca ccctggtgac agtaggcatt atgtttggcg cactgtttat gtggctactg
     5701 ggctaatttg tactttcccg tctcctctgt tcataattca aaccgtaact aataatgaga
     5761 ttatgttctg cacgccctgg gtatacgtaa caatggacaa atgtggtaca tttgcccgcg
     5821 ttgtcgcggt atccccaaca gaggatgtag agtcgtcttc ggatgcatgg gatgatgatg
     5881 ccgtttttca gggggcagga tgggtaaact aacgctgctg ttgctggcta ttctggtctg
     5941 gctacagtat tcgctgtggt tcggtaagaa cggtatacat gactataccc gcgtcaatga
     6001 tgatgtggcg gcacagcaag ctacaaacgc gaaacttaaa gcgcgaaacg atcaactttt
     6061 tgccgaaatt gacgatctca atggcggcca ggaggcgctc gaagagcgtg cgcgtaatga
     6121 actcagcatg accaggccgg gcgaaacttt ttatcgtctg gtgcctgacg cgtcgaagcg
     6181 cgcacagtct gcggggcaaa acaatcgata aatcagcccg ggaattaaca tggcaaccac
     6241 tcatttggat gtttgcgccg tggttccggc ggccggattt ggccgtcgaa tgcaaacgga
     6301 atgtcctaag caatatctct caatcggtaa tcaaaccatt cttgaacact cggtgcatgc
     6361 gctgctggcg catccccggg tgaaacgagt cgtcattgcc ataagtcctg gcgatagccg
     6421 ttttgcacaa cttcctctgg cgaatcatcc gcaaatcacc gttgtagatg gcggtgatga
     6481 gcgtgccgat tccgtgctgg caggtctgaa agccgctggc gacgcgcagt gggtattggt
     6541 gcatgacgcc gctcgtcctt gtttgcatca ggatgacctc gcgcgattgt tggcgttgag
     6601 cgaaaccagc cgcacggggg ggatcctcgc cgcaccagtg cgcgatacta tgaaacgtgc
     6661 cgaaccgggc aaaaatgcca ttgctcatac cgttgatcgc aacggcttat ggcacgcgct
     6721 gacgccgcaa tttttccctc gtgagctgtt acatgactgt ctgacgcgcg ctctaaatga
     6781 aggcgcgact attaccgacg aagcctcggc gctggaatat tgcggattcc atcctcagtt
     6841 ggtcgaaggc cgtgcggata acattaaagt cacgcgcccg gaagatttgg cactggccga
     6901 gttttacctc acccgaacca tccatcagga gaatacataa tgcgaattgg acacggtttt
     6961 gacgtacatg cctttggcgg tgaaggccca attatcattg gtggcgtacg cattccttac
     7021 gaaaaaggat tgctggcgca ttctgatggc gacgtggcgc tccatgcgtt gaccgatgca
     7081 ttgcttggcg cggcggcgct gggggatatc ggcaagctgt tcccggatac cgatccggca
     7141 tttaaaggtg ccgatagccg cgagctgcta cgcgaagcct ggcgtcgtat tcaggcgaag
     7201 ggttataccc ttggcaacgt cgatgtcact atcatcgctc aggcaccgaa gatgttgccg
     7261 cacattccac aaatgcgcgt gtttattgcc gaagatctcg gctgccatat ggatgatgtt
     7321 aacgtgaaag ccactactac ggaaaaactg ggatttaccg gacgtgggga agggattgcc
     7381 tgtgaagcgg tggcgctact cattaaggca acaaaatgat tgagtttgat aatctcactt
     7441 acctccacgg taaaccgcaa ggcaccgggc tgctgaaagc caatccggaa gactttgtgg
     7501 tggtggaaga tttgggcttt gagcctgatg gtgaaggtga gcatattctg gttagaatcc
     7561 tcaaaaacgg ctgcaatacc cgttttgtgg cggatgcact ggcgaaattc ctgaaaattc
     7621 atgcccgtga agtcagcttc gctgggcaaa aagacaaaca tgctgttacg gaacagtggt
     7681 tatgcgctcg cgtgccgggc aaggaaatgc ccgatctgag cgcctttcaa ctggaaggct
     7741 gccaggtgct ggagtatgcg cggcacaagc gcaagctgcg tttaggggcg ctgaaaggaa
     7801 acgcctttac cctggttctg cgcgaagtga gcaatcgcga tgacgttgaa caacgtctga
     7861 tcgatatttg cgtaaaaggt gtaccgaact acttcggtgc ccaacgtttt gggattggcg
     7921 gtagcaactt gcagggggcg cagcgctggg cgcaaaccaa tactccggtg cgcgatcgca
     7981 ataaacggag tttttggttg tcggcagccc gcagtgcgtt gtttaatcag attgttgctg
     8041 agcgcctcaa aaaagcagac gttaatcaag ttgttgacgg cgatgcgcta caattagccg
     8101 gacgtggtag ctggtttgta gcaaccaccg aagaactggc ggaattacag cgtcgcgtca
     8161 acgataaaga gttgatgata accgccgcat tgccaggcag tggcgaatgg ggaactcagc
     8221 gtgaagcgct ggcattcgaa caagcagctg tcgccgcaga aactgaatta caagctttac
     8281 tggtgcgcga aaaagttgaa gccgcgcgca gagcgatgct gctgtatccg caacaattaa
     8341 gctggaattg gtgggatgac gtcaccgtag agatccgttt ctggcttccg gcgggtagtt
     8401 ttgcaaccag cgttgtcagg gaacttatca acacaacagg tgattatgcg catattgctg
     8461 agtaatgatg acggggtaca tgcacccggt atacaaacgc tggcgaaagc cttgcgtgag
     8521 tttgctgacg ttcaggtggt cgcccccgat cgtaaccgca gcggcgcttc aaattctctg
     8581 acactggaat cctccctgcg cacgtttacc tttgaaaatg gtgatattgc tgtgcaaatg
     8641 ggaaccccga ccgattgcgt ctatcttggc gtgaatgctc tgatgcgtcc gcgcccggac
     8701 attgttgtgt ccggaattaa cgccgggccg aatctggggg atgatgttat ttattccggt
     8761 acggtagccg ccgcgatgga aggccgtcat ttaggttttc cggcgcttgc cgtctcgctt
     8821 gacgggcata aacattacga cactgccgcg gcggtaacct gttcaatttt gcgcgcactg
     8881 tgtaaagagc cgctgcgcac cgggcgtatt cttaatatta acgttccgga tttacccttg
     8941 gatcaaatca aaggtattcg cgtgacgcgc tgcggtacac gacatccggc agatcaggtg
     9001 atcccgcagc aagatccgcg cggcaatacg ctgtactgga ttggcccgcc gggcggtaaa
     9061 tgtgatgctg gtccggggac cgattttgct gcggtagatg agggctatgt ctccatcacg
     9121 ccgctgcatg tggatttaac tgcgcatagc gcgcaagatg tggtttcaga ctggttaaac
     9181 agcgtgggag ttggcacgca atggtaagca gacgcgtaca agcacttctg gatcaattac
     9241 gtgcgcaagg tattcaggat gagcaggtgc tgaatgcact tgccgccgtg ccgcgtgaaa
     9301 aattcgttga tgaagcgttt gaacaaaaag cctgggacaa tatcgctttg ccgataggtc
     9361 aggggcagac aatttcgcag ccatatatgg tggcgcgaat gaccgaatta ctcgagctga
     9421 cgccgcagtc gcgggtgctg gaaattggca ccggttcggg atatcaaacg gcaatcctgg
     9481 cgcatcttgt ccagcatgtt tgctcggttg aacggattaa aggcttgcag tggcaggcac
     9541 gtcgccgcct gaaaaatctt gatttacata atgtttcaac ccgtcatggc gatggatggc
     9601 aaggttggca ggcacgtgcg ccgtttgacg ctatcattgt tacggcggca ccgccggaaa
     9661 ttccaactgc gctaatgacg cagctggacg aaggcgggat tctcgtctta cccgtagggg
     9721 aggagcacca gtatttgaaa cgggtgcgtc gtcggggagg cgaatttatt atcgataccg
     9781 tggaggccgt gcgctttgtc cctttagtga agggtgagct ggcttaaaac gtgaggaaat
     9841 acctggattt ttcctggtta ttttgccgca ggtcagcgta tcgtgaacat cttttccagt
     9901 gttcagtagg gtgccttgca cggtaattat gtcactggtt attaaccaat ttttcctggg
     9961 ggataaatga gcgcgggaag cccaaaattc accgttcgcc gcattgcggc tttgtcactg
    10021 gtttcgctat ggctggcagg ctgttctgac acttcaaatc caccggcacc ggtcagctcc
    10081 gttaatggca atgcgcctgc aaatactaat tctggtatgt tgattacgcc gccgccgaaa
    10141 atggggacga cgtctacagc gcagcaaccg caaattcagc cggtgcagca gccacaaatt
    10201 caggctactc aacaaccgca aatccagcca gtgcagccag tagctcagca gccggtacag
    10261 atggaaaacg gacgcatcgt ctataaccgt cagtatggga acattccgaa aggcagttat
    10321 agcggcagta cctataccgt gaaaaaaggc gacacacttt tctatatcgc ctggattact
    10381 ggcaacgatt tccgtgacct tgctcagcgc aacaatattc aggcaccata cgcgctgaac
    10441 gttggtcaga ccttgcaggt gggtaatgct tccggtacgc caatcactgg cggaaatgcc
    10501 attacccagg ccgacgcagc agagcaagga gttgtgatca agcctgcaca aaattccacc
    10561 gttgctgttg cgtcgcaacc gacaattacg tattctgagt cttcgggtga acagagtgct
    10621 aacaaaatgt tgccgaacaa caagccaact gcgaccacgg tcacagcgcc tgtaacggta
    10681 ccaacagcaa gcacaaccga gccgactgtc agcagtacat caaccagtac gcctatctcc
    10741 acctggcgct ggccgactga gggcaaagtg atcgaaacct ttggcgcttc tgaggggggc
    10801 aacaagggga ttgatatcgc aggcagcaaa ggacaggcaa ttatcgcgac cgcagatggc
    10861 cgcgttgttt atgctggtaa cgcgctgcgc ggctacggta atctgattat catcaaacat
    10921 aatgatgatt acctgagtgc ctacgcccat aacgacacaa tgctggtccg ggaacaacaa
    10981 gaagttaagg cggggcaaaa aatagcgacc atgggtagca ccggaaccag ttcaacacgc
    11041 ttgcattttg aaattcgtta caaggggaaa tccgtaaacc cgctgcgtta tttgccgcag
    11101 cgataaatcg acggaaccag gcttttgctt gaatgttccg tcaagggatc acgggtagga
    11161 gccaccttat gagtcagaat acgctgaaag ttcatgattt aaatgaagat gcggaatttg
    11221 atgagaacgg agttgaggtt tttgacgaaa aggccttagt agaagaggaa cccagtgata
    11281 acgatttggc cgaagaggaa ctgttatcgc agggagccac acagcgtgtg ttggacgcga
    11341 ctcagcttta ccttggtgag attggttatt caccactgtt aacggccgaa gaagaagttt
    11401 attttgcgcg tcgcgcactg cgtggagatg tcgcctctcg ccgccggatg atcgagagta
    11461 acttgcgtct ggtggtaaaa attgcccgcc gttatggcaa tcgtggtctg gcgttgctgg
    11521 accttatcga agagggcaac ctggggctga tccgcgcggt agagaagttt gacccggaac
    11581 gtggtttccg cttctcaaca tacgcaacct ggtggattcg ccagacgatt gaacgggcga
    11641 ttatgaacca aacccgtact attcgtttgc cgattcacat cgtaaaggag ctgaacgttt
    11701 acctgcgaac cgcacgtgag ttgtcccata agctggacca tgaaccaagt gcggaagaga
    11761 tcgcagagca actggataag ccagttgatg acgtcagccg tatgcttcgt cttaacgagc
    11821 gcattacctc ggtagacacc ccgctgggtg gtgattccga aaaagcgttg ctggacatcc
    11881 tggccgatga aaaagagaac ggtccggaag ataccacgca agatgacgat atgaagcaga
    11941 gcatcgtcaa atggctgttc gagctgaacg ccaaacagcg tgaagtgctg gcacgtcgat
    12001 tcggtttgct ggggtacgaa gcggcaacac tggaagatgt aggtcgtgaa attggcctca
    12061 cccgtgaacg tgttcgccag attcaggttg aaggcctgcg ccgtttgcgt gaaatcctgc
    12121 aaacgcaggg gctgaatatc gaagcgctgt tccgcgagta agtaagcatc tgtcagaaag
    12181 gccagtctca agcgaggctg gccttttctg tgcacaataa aaggtccgat gcccatcgga
    12241 cctttttatt aaggtcaaat taccgcccat acgcaccagg taattaagaa tccggtaaaa
    12301 ccgagaatgg tcgttaacac tgtccaggtt ttcagaccgt ctgctaccga caaccccaga
    12361 tatttggtca caatccagaa ccctgagtca ttaatatgtg acgcgccaag cccaccaaag
    12421 caggctgcca gcgtcaccaa tacgcactga atcggattca atcccatcac cgcttctgag
    12481 agtaacccgc cggttgtcag gattgctacg gttgctgacc cctgcgatgc acgcagagcc
    12541 agtgaaataa taaatgcggc tggtaacaga ggcaggtcaa tcatttgtag catattggca
    12601 agggctttgc cgacgcccga ttccaccagc actttgccaa atacccctcc agcaccagta
    12661 accaaaatca ctaccgccgc agtgggaagg gctgagccca taatgtcgct ggtgtgttgt
    12721 aagctccagc cgcgacgtaa agccaataac cagaatgcca gcaccagcgc aatcattaga
    12781 gctaccattg gtgagccgat cagctgtagc gtaccaagca ggggatgcga aggcggcatc
    12841 agtgttgcgg aaaccgtacc cgccatgata atcgcgatag gaataacaat tagcgaggtg
    12901 accagcgcga cgcccggtgg atttatttta tcgcttaatt ttgtcgcgcc ttcctcactg
    12961 gccggagcca gttgcatctg ttccagtact tccaccgaca tcgcatattg gtgcttattg
    13021 attattttcg ctgcaaagta gccaacaatc cctacgggaa tagaaatcgc aataccgatg
    13081 atggttagcc agccgatgtc tgcgtggagt aaccccgctg cggcgacagg gcctggatgc
    13141 ggcggtaccg ccacatgtac agttagcatg atcccagcga caggcaggcc aaatttgagt
    13201 ggcgatattt tggcaacctt ggcaaaaccg taaatgattg gcgcaagaat aataaagccg
    13261 acatcaaaga agacgggaat accgaggaag aacgctgcca gagtcagcgc agcgatagtt
    13321 cgtttgtcac ctaacttgcg actgaaataa ttagccagtg actctgcacc accagagtgt
    13381 tcgatcatac gccccagcat agcgcccaga ccaataataa tagtgacgga accaagcaca
    13441 ccgcccatcc cggcgatcat cactttaccc acttcgcccg ccggtatacc cgccgcaagt
    13501 gcgactaaca ggctgacgag gagcagagca acgaatggtt gtacctttgc cttgatgacc
    13561 agcagcaaca gcatgattac gccagttaac gcaatgcata acaatgtaat tgtggacatg
    13621 ggaaaccctg tctgaaagtt atagttaacc taccccatcc gtagatgggg ggatgtatgg
    13681 gtacgttgta attagggatt taacgaatta gcgccaggcg tcaaaccagc caagcccttc
    13741 ttcggtgagg ccacgaggtt tatattcaca accgatccag ccctgatatc ccacctcatc
    13801 gaacaggcgg aacagccacg gatagttgat ttctccatcg tccggttcat gtcgatcagg
    13861 tagtccggca atttgtacgt gcgcatattt cccggcgtag tcgcggatta aatgcgtcag
    13921 gttgccatct actttttgcg catgaaaagt atctagttga ataaacacgt tatctcgcgc
    13981 aacctcttca acaatagcca gtgcctgata ctggctggag aagagataat gaggcttaac
    14041 gtcggggctg agtgcttcaa ctaatattcg cttgccgtgt agcgcaaagc ggtcggcagc
    14101 gtagcggaga ttatcgataa atactgcccg gtaccgttca gcgtcttcgc cagcgggcac
    14161 gacgcctgcc atcacatgga cttgttcaca attgagcgcc aatgcatatt ccagtgccag
    14221 gtcgatgtct gcgtgtgctt cgtgctcacg tccgggaagg gcggataatc cccattcccc
    14281 cgcattaata tctccgggag cggtattgaa cagcgccagt gtcagatggt tttgctccag
    14341 ttgcttttgg atttgcaggg tggagtagtc atagggaaac agaaattcca cagcatcgaa
    14401 cccggctttt cgcgctgcgg cgaagcgttc aataaaaggc acttcggtga acatcatgga
    14461 taaattagct gcaaaacgag gcattgcatt aactccttaa ttccgcaatt tcacctgcgg
    14521 tcagataacg gatcgggcgg tcaccgagaa taaaaatcag ctttgccgtt tcctccagct
    14581 cttccatatt gttggcggct tcttgcaggc tttcaccgca aaccactggg ccatgatttg
    14641 ccagtaaaaa agcctgattg tctgctgcca gttccgccag atcctgtgcg atgcgtttat
    14701 cgcccggtcg gtaataaggc accagcggga catttcccat ccgcatcacc acgtatggtg
    14761 tgaacggacg aataacgttg ctgctgtcca gcccttgcag gcaggaaagc gccgtcgacc
    14821 atgtgctgtg caaatgcacc accgctttac agcgcggatt gttgcgatac agcgccagat
    14881 gaaagagcac ctctttcgag ggtttgtcac cacttaacca ttcgccatcc gcggcgactt
    14941 tggaaagccg ctgcggatcg agattgccca ggcatgaacc tgtcggtgtc gccagtaaat
    15001 tcccgtcagg taaaagcagc gacagattgc cagccgaacc ggttgcatag ccgcgctgaa
    15061 agaatgaact ggcaatccgc gtcatctcct ctcgcaaaga ctgctctact tttgcgaaat
    15121 cgctcatgat aaaaactctc tttgggctcg tgaaaaaaag gcttcatcac cgaagttgcc
    15181 agatttaagg gcgagtgaga caggcttatc cagtgcgttt acccacggca cgccggggga
    15241 aatggttggg ccaatatgaa acccttttat gcccaggctc tgtgtgacta cgccggaggt
    15301 ctcaccgcct gcgacaataa agcgtgtcac gccttccgct gctaaccgcg ccgctagttg
    15361 agaaaacaga gtttctactg cctgactggc tttttgtgca ccgtattgct gttgaattgc
    15421 tgccaatgcg tcagtgctgg cggtggcaaa aaccagtgga gcaagtacac tttcctggcc
    15481 cagaacccac tctgccagtt cgtgtgcata agcggccaga gtttcggttg agaggcagcg
    15541 tgccacatca acttcacggg ctggtgcaat ttgacggtaa tgtgccacct ggcggttggt
    15601 catttgagag catgaaccgg agagcactac gccgcgccca gcgagcggat gccctgcttc
    15661 gcgagcctgg ttaccgtttt cttgcgccca ctgccgggcc aggccaatcg ccagaccaga
    15721 accgcccgtt accagtgggg catcgcgcaa ggcttctccc tgaatttcca gatggtgttc
    15781 ggtcagcgca tcaagcaccg cgtagcggta gccctcttgc tgtaagcgag ccagctcttg
    15841 acgaacggca tccacacctt gttcgaaaac atgtgccgaa acgacgccgc agcgccctgt
    15901 ggattgcgct tcaaccagac ggggaagata gctgtcggtc atgggattta ccgggtgatg
    15961 gcgcatcccg gattcggcca gcagttgatt cattacgaac aaatacccct gataaaccgt
    16021 acgtccgttg accggcaggg ccggagagaa gaccgtaaac ggcgtgtcga gagcatccat
    16081 taatgcatcg gtaaccgggc cgatattacc tttcgccgta ctgtcgaaag tagagcagta
    16141 tttgaaatag atctgtttgc aaccttgctg ttgcaaccag ctcagagccg ccagcgattg
    16201 ctgtgtggct tcaaccaccg gacaggagcg cgttttcagg ctgatcacca gtgcgtcgat
    16261 tgcttccggc attttacctg ttggaacacc gttaatttgt accgttggta gaccgttttc
    16321 caccagaaaa ctggcgatat ccgtcgcgcc ggtaaaatca tcggcgataa cgccaatctt
    16381 gatcatgatt tcgctcccgg tagagtgatg ccagagaaaa tcttgataac tgcgctatcg
    16441 tcttctttcc cgtaacctgc gttactggcg ctggtgaaca tattcaatgc tgttgaggcc
    16501 aatggcagcg ggaagtgcag ggctttggct gtatcggcaa ccagaccaag atccttaaca
    16561 aaaatatcga cggctgaatg cggggtgtaa tcgccatcca ccacatgacg catccggttt
    16621 tcgaacatcc aggaatttcc ggcggcattg gtcacgacgt catacatcac atccagcggg
    16681 atccccgcac gggctgcaag tgccatcgct tcggctccgg cagcaatatg tacgcccgct
    16741 aacaactggt gaataatttt tacggtcgaa cctagtcccg gttctgcacc tatgcgataa
    16801 acttttccgg caacggcttc cagcacgggt gccagtcgtt caaaggcaat atcgctaccg
    16861 gaggccatga cagtcatttc accgttagcg gcttttactg caccaccaga aactggcgca
    16921 tccagcattt ccagatcgaa tccagccaga gcggtagcaa tttcttgcgc atcagcacta
    16981 gcgatagtgg aagaaaccat tactgccgta ccgggtttca gatgttgtgc aacgcctgtt
    17041 tcaccaaaca gcacctgttt aacctgggcc gcattgacca ccagcaccag cagagcgtcc
    17101 agtttttcgg caaacgtcgc ggcgttatca gaaaccccgc aagcacctgc ctctttcaac
    17161 gtagcgcagg cattgctgtt caggtctgcg ccccaggtag aaagacctgc gcggacacat
    17221 gacagtgctg ctcccattcc cattgaccct aagccaacga taccgacatg aaactcagat
    17281 cccgttttca tctgctctcc ttgttaattt aagtgatatt ttgtttgata ttgtgaatat
    17341 aagcgctgga agataacgat atggtgagct gattcacata aattaacatt gtgtgttatt
    17401 ttatgtgaac taagcgttag ttgcgccgcg cacgtttcgc aggcaaatag cgtagaatgt
    17461 cagcaggaca aaggaaggag caaaagttga tacccgtaga gcgtcgccaa atcatccttg
    17521 agatggtagc tgaaaaaggc attgtcagta ttgctgaact aacggacaga atgaatgtgt
    17581 cacatatgac cattcgtcgg gatttacaaa aactggagca gcagggagcc gttgtgctgg
    17641 tgtccggagg cgtccagtct ccgggacgcg tggcgcatga accttctcat caggtaaaaa
    17701 ctgcgctggc aatgacgcaa aaagcggcta ttggcaagct ggcggcaagt cttgttcagc
    17761 cgggaagctg tatctatctg gatgcgggaa cgaccacgtt agcgatagca cagcatctga
    17821 ttcacatgga gtcactgact gtggtcacaa acgatttcgt tattgcgaac tacttgctcg
    17881 acaacagtaa ttgcacaatt attcacactg gcggtgcagt gtgtcgggaa aaccgttcct
    17941 gtgtcgggga agccgctgcg accatgctgc gcagcctgat gattgatcag gcttttattt
    18001 ctgcatcgtc atggagtgtg cgggggattt ctacgccagc ggaagataaa gtcacggtga
    18061 aacgggcgat tgccagtgcc agccgccagc gagttttggt ctgtgatgcg acgaaatatg
    18121 gtcaggtggc gacatggctg gcgttaccat taagcgagtt tgatcagatt atcacagacg
    18181 acggtttgcc ggagagtgcc agtcgcgcgc tggcgaagca ggatctctct ttgctggtag
    18241 cgaaaaatga ataatggcct gcaataacat ttggttactc atgcttcaca gaagaagcat
    18301 gagactactt tattttataa aatgacagcc gcccgctttt cggcgtgccg gtatcaatat
    18361 aaatctggtt agcgaacgtc tgaatgttat caaacatcat atgtccaaat ataaaataat
    18421 cagcgccgtt tatttgttgt aactcgccat taagcgattt ctgcacacga tcaacaggcc
    18481 agagtaattc gctctccgct atttctttac caaagagata ttcactcccc ggataatctg
    18541 catgtgcgat gacatatttt atgttgtcgt tagtgatttc aataatatgt ggaaggtgat
    18601 ggaatttcag caacagatct attgcctctt gttgctctga atcatttaaa tcgaaaaacc
    18661 agtcaccacc gctggcaagc cacatattgc catcgccagt ttcgaatgca tccaacgcca
    18721 tcgcttcgtg gttgccttta accgacgtaa accagggttg gtttagcagg cgcagcacgt
    18781 taagactctt cggcccacga tcaatattat cgcctgtgga aataagtaag tcggtttcag
    18841 ggtaaaaaga gagttgatgt aaacgggatt gtaataactg atattcacca tgaatatcac
    18901 caacgaccca tatatggcga tagtgatggg cattgatttt ttgatagcgt gtagatggca
    18961 tggttttacc ctgtaaaata agctttccta ttatacaggg tatttttatt tgattcgtca
    19021 gttgtcgtta atattcccga tagcaaaaga ctatcgggaa ttgttattac accagactct
    19081 tcaagcgata aatccactcc agcgcctgac gcggagtcag tgaatccggg tcgagatttt
    19141 ccagtgcttc gaccgcaggc gaagtttctt ctggcactga cagcaaagac atctgcgtac
    19201 catccacttg cgtcgcggcg gcgttcggcg aaatgctttc cagctcacgc agtttttgcc
    19261 gtgcgcgctt aatcacctct tttggcacgc ccgccagagc tgcaaccgcc aggccgtagc
    19321 ttttgctcgc cgcgccatcc tgcacgctat gcataaaggc aatggtgtcg ccgtgctcca
    19381 gcgcatcgag atgcacgttg gcgacgcctt ccattttctc cggtaactgg gtcagctcga
    19441 aatagtgggt agcaaataac gtcaatgcct taatcttatt cgccagattt tccgcgcacg
    19501 cccacgccag cgacagacca tcgtaggtgg acgttccacg cccgatctca tccattaaca
    19561 ccagactgta ttcggtggcg ttatgtaaaa tattggcggt ttcagtcatc tccaccataa
    19621 aggttgagcg cccggaagcc aggtcatctg ccgcgcctac gcgggtaaag atgcgatcga
    19681 taggtccaat ctcgactttt tgtgccggta catagctgcc gatgtaggcc atcagcgcaa
    19741 tcagtgcggt ctggcgcata taggtacttt taccgcccat gttcgggccg gtaataatca
    19801 acatacggcg ctgcggcgac agattcagcg ggttagcgat aaatggctca ttcagtactt
    19861 gttcaactac cggatggcga ccttcggtaa tgcgaatgcc cggtttatca atgaaggtcg
    19921 ggcaggtgta gttcagggta taggcccgtt ccgccaggtt aaccagcacg tcgagttccg
    19981 ccagcgcgct cgcgctctgt tgcaacgctt ccagatgcgg caacagcagg tcgaacagct
    20041 cttcataaag ctgtttttcc agtgccagtg ctttgccttt tgaggtgaga actttatctt
    20101 cgtactcttt tagctctgga atgatgtagc gctcggcgtt tttcagcgtc tggcgacgca
    20161 tgtagttgat gggtgccaga tggctttgcc cacggctgat ttgaatgtag tagccgtgca
    20221 ccgcattaaa gccaactttc agcgtgtcca ggccggtacg ttcacgctcg cggacttcca
    20281 gacgctccag ataatcggtc gcgccgtcag ccagcgcgcg ccactcatcc agctcttcgt
    20341 tatagcccga tgcgataaca ccaccgtcgc gtaccagcac cggcggtgtg tcgatgattg
    20401 ctcgctccag cagatcgcgc agctcggcaa actcgcccat cttctcacgt agcgcctgta
    20461 ccggtgcact atcgacagtt tctaactgcg cacgcagctc cggcagttgc tggaaagcgt
    20521 ggcgcatacg ggccagatcg cgtgggcgag cagttcgtaa agccagacgt gccagaatac
    20581 gttccaggtc gccgacctga cgcagtaccg gctgcaactc ggcggtgaaa tcctgcaatg
    20641 cgccaatagt ttgctggcgc tcaagcaaca cgcgggtatc gcgcactggc atatgcagcc
    20701 agcgtttcag catacgacta cccatcggcg tgacggtgca gtcgagcaca gaagccagcg
    20761 tattttccgc accgcccgcc aggttctgag taatttccag gttacgacgt gtcgcggcat
    20821 ccataatgat gctgtcctgc tcacgttcca tggtgataga acgaatatgc ggcagggtcg
    20881 tgcgttgggt atctttcgca tactgcaaca gacaaccggc agcacaaagt ccgcgcggcg
    20941 cgttctcgac gccaaaaccg accagatcgc gggtgccaaa ttgcagattc aactgctggc
    21001 gcgcggtgtc gatttcaaac tcccacagcg ggcgacggcg caggccgcga cggccttcaa
    21061 tcagcgacat ctcggcgaaa tcttctgcat acagcagttc cgccggatta gtgcgttgca
    21121 gctctgccgc catcgtttcg cggtcggccg gttcgctcag gcgaaaacgc ccggagctga
    21181 tatccagcgt cgcgtagccg aaacctttgc tgtcctgcca gatagccgcc agcaggttgt
    21241 cctgacgctc ctgtaacagg gcttcatcgc tgatggtgcc tggcgtaacg atacgcacaa
    21301 ctttgcgctc aaccggacct ttgctggtcg ccggatcgcc aatttgttcg cagatggcaa
    21361 cggactctcc ctgattcacc agtttggcga gatagttttc caccgcatgg tagggaatcc
    21421 ccgccatcgg gatcggctct cccgccgaag caccgcgttt ggtcagtgaa atatccagca
    21481 gttgcgacgc gcgttttgcg tcgtcataaa acagttcata aaaatcaccc atccggtaaa
    21541 acagcaggat ctcgggatgc tgggctttca gcctgagata ctgctgcatc atgggcgtat
    21601 gggcgtcgaa attttctatt gcactcatgg ggttatgtcc ggttccctga tattaaatgg
    21661 ggtgtgatgg tttttatttt cgttgtaatc acataaggcg cacatgatac cggagttaat
    21721 catttcaggg gaagaagcga acggcgagtt cttgatgctg gttccgggaa agatgctgtt
    21781 ttgcagcttt tgcgtgatgc ttatgaagca agtacgcaaa attgcgtcgg ttgaataaac
    21841 ctcctccttc agagccatag tcccctttca aattaagttg taatataaat acaccttaag
    21901 agtatggagg cagcatgtcc ggcaagcgta tctctcgtga aaaactgacg attaaaaaaa
    21961 tgatcgatct ttatcaagcg aaatgcccgc aggcgtcagc ggagccggag cattacgagg
    22021 cgttgtttgt ttacgcgcaa aagcggctgg ataaatgtgt gttcggcgaa gagaaaccag
    22081 cctgtaagca gtgtccggtg cactgttacc agccagcaaa gcgtgaagag atgaaacaga
    22141 ttatgcgttg ggccgggccg cgaatgctgt ggcgtcatcc gatcttaacc gtgcgtcatc
    22201 tcatcgatga caagcgtccg gtgccagaac tgccggaaaa atatcgtccg aaaaaaactc
    22261 gtgaatagta tgccggacag gcagatctgt ccggcaattt gcagttaaat caatgccgat
    22321 ttatcaattc caagccgctt catccgtgac agcaaggtcg tgcgtttcag ccccagacgt
    22381 tgcgcagcgc ctttaggccc ggcaaccacg ccgttggttt ctttcagcac gcgaacaatc
    22441 aactgatatt catcttcgcc ctcctgggcg acaaccgttg cggcaggcgg cgtttcaggt
    22501 tccggtaaaa caatatctgg caatgacagc tgcagcacgt taccgcgtgt tagcaatacc
    22561 gcgcgctcaa tgacgttttc cagttcgcgt acgttacccg gccactccat gttgctcaag
    22621 gtgcgcagcg tctcggcagg aatgctgtcg atattgcgcc ccagacgacg ggcaattttg
    22681 aaggtaaagg ctttcgccag cagcggaata tcttccggac gctcgcgtag tggcggcagg
    22741 tgaatcggga atacgttcag gcggtaatag agatcgctac ggaactcacg gtcggcgacc
    22801 atttttttca gatcgcggtt agtcgcggcg attagacgca cgtccgtctg aatgattttg
    22861 ttgctgccga gacgttcaaa ctcctgttcc tgcaatacac gcagcaactt cggctgtaac
    22921 tccagtggca tatcgcccac ttcgtcgagg aacagggagc ttttatccgc cagttcaaaa
    22981 cgaccaatgc gctgggcgct ggcaccggta aaagccccac gctcatgacc aaacagatcg
    23041 ctttccagca atccggcagg catcgccgcg cagttcattt tgaccatgcg gcgattatta
    23101 cgcccactga gattatggat cgcacgggca atcagctctt tacccgtgcc agtttcaccg
    23161 aggatcagca cggtactgtc actttgcgcc accatttcaa cttgtttaag cacgctgtac
    23221 atggcttcgc tgcggccaat aatctcgcca aattcactat caacattgtt gagctgctcg
    23281 gtcagggcga ggttttcatc aaccagccgt tctttcagac gatggatttc ctgataggcg
    23341 agggcgttat cgacagcgat tgccacacgt tcggcaatct ggcgcagtaa attcagattg
    23401 gtagtggtaa acactttctc ttcgcattgc gccagtttca gcacgcccag catggtgtcg
    23461 ccagacatca gcggtaacag gcacaaggtt tgaatctggt tgccccaggt gtcgaacaac
    23521 atgcgttcat agggggctaa atcgtcccgc tcgtggagat tgatcagcag catctcttta
    23581 cttttgaaca cgcgttcggt gagggttccg gcttcatcga cttcgctctg ttcgtgggcg
    23641 ggatgttgtt tatcaagata gtgagtggag tagatgttga gtttgttttt acggtggctg
    23701 cgtaagacaa tgctgatatc gtcaatgtcg aaatagtaat ggatttcttt ggcgacttcg
    23761 ctgaccagtt cgtccatatc caggcgggaa agcaccgcgt tggtaatggc aaccaggatg
    23821 cggaagttat cgcgctcccg gcataacaac tcatagtcga cattgttgac gacgcggctc
    23881 tggatttgtt cggtgacgac agaaacgatc tgcgtaaatg tttgcagacg attgaactct
    23941 ttttcgctcc aggggcgatc gtcataacga ataaattcac agccaccaaa aatatgccct
    24001 tccgccgcca gtggcatcag gcaatagtga ccaaatttgg ggtatagccc acctgcggcc
    24061 agctgcggcc aggtttcaca aaattcttcg taactgcaat gcagcgtatc agggcgcgac
    24121 aaaatgctgc gtaccggacc gtgtgccaga acagtttcgt cttcatattt aatgggggtg
    24181 tctttttcac gcgatgcgta ataagacgca cgttgagtct gcgcttgcca caacacaata
    24241 gccgcgttgt cggcgagcgc agaacgcttt accagttgcg aaagagcctc acacagcgag
    24301 gccagatcgg gctgctgcaa tagtgtccga gtgatgtcga acaacccttg ctgtccgaga
    24361 tcactcatcg gtgtatatga cattttgttc ttccaggaaa gcaccgcaaa gcggtgcaaa
    24421 aggtcattga ctaactatag gagatttaga attttattag catatacgcg gaagcggttc
    24481 ggcgtgtggt aaatcgaggg ttcgtttcac gccatacaga ccggcaagac gaacaccttt
    24541 acgttccacc acttcaccaa tcagcgccgc gtctttcccc agtggatggg aatgtaacgc
    24601 tgccagcact tgctctgccg cgttgcgttc aacagctatt actagtttgc cttcgttggc
    24661 aaagttcagg gcgtccagtc ccagcaattc gcaaacgcca cgcacggcag gtttaacagg
    24721 cagtgccgct tctgaaagtt caataccaca accgcaggct gccgcgaact catgaaccac
    24781 cgcgtttaca ccaccacggg tggcatcacg cagcgctttc acgccgggaa tgtcacgcag
    24841 cgtctgaata agcggcgtca gcaccgcgca gtcgctgacc agttcgccat ccagccccag
    24901 ctgctcacgc aggttaagga tagtcgcccc gtggtcgccg agtgtaccgc tcaccagcaa
    24961 tacatcgcct gcggttagcg tctgtgcgcc ccagtgaata ttcgccggaa ttgcgcccat
    25021 gccagcggtg ttgataaaca gtttatctac cgcgccgcgc tgcaccactt tagtatcgcc
    25081 agtaacgatg gcaatgcctg ccgtgcgggc ggtttctgcc atgctggtca ctacggcttt
    25141 tagtgtctcc atcggcaatc cttcttcgag gataaagcca caggagagat agcgcggaat
    25201 agcgccactg accgcaacgt cattcgcggt gccgcaaatc gccagcttgc cgatattacc
    25261 gccaggaaag aacagcgggt caatgacgta actgtcggtg gagaacgcca gacgatcgcc
    25321 ttctgctacc agctgcgcca gatcaagacg tgcctggtct tcctgctctg ccagccacgg
    25381 gttggcaaag gcttccataa acaggctgtt gattaattgc tgcatcgcct ggccgccgct
    25441 gccgtgggcg agttggatat tattcacgct tcattctcct gctgacgata ctgataccac
    25501 gcggcgcacg ctccttcgga ggagaccatc agtgcaccaa atgcggtttg aggattacag
    25561 gtgttaccaa acagcgggca ttgatgcggc ttacatttgc ccgttaatac ttcaccacaa
    25621 cgtgcgcgcg ggtcatcgca gacctgctgc ggtgccgggc ggaaatgtgc ttcggcatcg
    25681 aatcgttgat agtccggcgt caggtgtaca ccagaagatt caatcacgcc taagccgcgc
    25741 cattcgctgt cgccgttgac gcagaagaca tccgcaatcg cctgttgcgc cagcaggtta
    25801 ccggcatccg gcactacccg ccgatactga ttttctacct tgctatgagc agctattttc
    25861 tgctccacca gcatgaccac gccttgtagt agatcaaggg gttcgaatcc agcaaccacc
    25921 agcggacgat gaaaatcgct ggcgataaaa ttataggcgt tggtgccgat aaccatactg
    25981 acgtgacctg gcgcgaggaa cgcatcgata ccgttatccg gctgttccag caaactgcgc
    26041 aacgtcggga taagcgtaat gtgctggcag aagaagtaaa aattctgcac atcacgcgct
    26101 ttcgcctgtt gcagagtgat ggcggtggtc ggcatggtgg tttcaaaacc taagccgaag
    26161 aacaccactt tgcgggttgg attctcctgc gccagtttca acgcatccat tggcgagtaa
    26221 acgatgcgaa catcggcacc gcgtgctttt gcctgcaaca gcgatccctg tttccccggc
    26281 acgcgcatgg cgtcgccaaa ggtacagaag atgacttccg gatggctggc aatctccacg
    26341 caggtgtcga ttctacccat cggcagtacg cacaccgggc aacccggacc gtggataaac
    26401 tcaacgtttt caggcagtaa ctggtcgagg ccgaatttaa aaatggcgtg ggtatgacca
    26461 ccgcacactt ccataatccg cagagggcgt tcggctgtgt aagagagatg tgaagcacgt
    26521 tcgcgcagat gctcaattaa ctgcatcacc tgttccggcg cgcgatattc atcaacaaaa
    26581 cgcattattt ttcctcgcca tacaacagcg cgccgacatc cggctcaacg tcaaacatgt
    26641 tttgtaaggc gtcgagagtg tcgcgtgctt cggcttcatt aattacgctc atggcaaagc
    26701 caacgtgtac cagtacccac tggcccacgc gcggctgacc gttttcatcg cagctgccga
    26761 ctaacgttaa atcgacatcg cgctgaatgc cgcagacgtc gactttcgcc tggttgccgt
    26821 caatggtgcg gatctggccg ggaacgccta tgcacatcgc tgtgtctcca gccagttcag
    26881 ccactggtcc atcccttcgc cgctggtggc ggaaataagg atgatttcaa tttctggatt
    26941 gacttcgcgg gcgcaggcga tgcacttctc aacgtcaaag ttgagatacg gcagcaggtc
    27001 aactttgttg agcagcatca gcgaggcggc ggcaaacata tgcggatatt tcagcggttt
    27061 gtcttcacct tcggtaacgg aaagcaccgc cactttgtgt ttttcaccga gatcgaagct
    27121 ggccgggcat acgaggttgc caacgttttc gataaacaga ataccgttat cgtccagtgg
    27181 caggcgcggt gcggcttcgg caatcatctg tgcgtcaaga tggcagcctt taccggtatt
    27241 cacctgaatc gctggtgtgc cggtagcgcg aatgcgtgcg gcatcgttca cggtttgctg
    27301 atcgccttca ataactgcgc acggaacgct gtctttcagg cgcattaggg tttccgtcag
    27361 cagggtggtt ttaccggaac cagggctgga aaccaggttg agcaccagtt gcttgcgggc
    27421 agcaaagcgc gcgcggttgc gttcagccag acggttattt ttgtccagca cgtcaatttc
    27481 gacttccagc atccgacgct ggctcatgcc cggcgcgtga gtgcctgctt cgccatgacc
    27541 gtaatgcagg tcgccttctt cagtctggct gggggtaaat tcaggcgctt taatgccggt
    27601 gattttcatc ttcgggcgtg ccgccggggc aaatggcgcg ctacgaaacg cggaatgagg
    27661 gttatgttca tcaccctcga tatacaggtt gccttcaccg caaccgcatg ttgtacacat
    27721 cgctcactcc tggtctattt ctatccgccg aatctgtaaa ccgtcgtctg ccacaatctg
    27781 tagcatgtca ccatgacact gtggacagcg gcggacgcgc tgggtcagta gcgtcacata
    27841 ctgttggcat gtttcacacc agcattcggc ctcttgttct tcgaggtgca gtttacaacc
    27901 ttccgccacg ctgccgcggc aaaccagatc aaaacaaaag gcaagagagc tggtttcgac
    27961 acaagaaaat gcgccaattt tgagccagac cccagttacg cgttttgcgc cgtgttttgc
    28021 ggcctgctgt tcgatcaatt ccagtgcccg ttggcagagg gttatttcgt gcatatcgcc
    28081 tcccattaac tatggccagc tacaagcaat aattgtgcca gtattgatta tccctgcggt
    28141 gaataatgtc gatgatgtcg aaatgacacg tcgacacggc gacgaaattc atctttagct
    28201 taaaaatcgt tttaataaca atgaattaaa agttggcaca gaaaatgctt aaagctggca
    28261 tctctgttaa acgggtaacc tgacaatgac tatttgggaa ataagcgaga aagccgatta
    28321 catcgcacag cggcatcgtc gcctacagga ccagtggcac atctactgca attcgctggt
    28381 tcaggggatc acgttatcga aagcgcgcct gcatcacgcc atgagctgcg cgccggacaa
    28441 agaactctgt ttcgtccttt ttgaacattt tcgcatttac gtcaccctgg cggatggctt
    28501 taacagccac accatcgagt attacgtcga aacaaaagat ggcgaagaca aacagcggat
    28561 tgcgcaggcg caactgagca ttgacggcat gattgatggc aaggtcaaca tccgcgatcg
    28621 cgaacaggtt ctggaacact atctcgaaaa aatcgctggc gtttacgaca gcttatacac
    28681 cgctattgaa aacaatgtgc cggtgaattt aagccaactg gtaaagggac aaagcccggc
    28741 agcatgagct gaggctttgc ccgttttgca ggcgttacgc ctgtttgggg atgggcgtgt
    28801 cgatgagtgt cgaaaatgac atttcatcgg catgttttcg tcaaaaatga caatcacctg
    28861 aggaatgcct ggtgaatcgt tttgtaattg ctgactccac gctctgtatc ggctgccaca
    28921 cttgtgaggc cgcctgttca gagacgcatc gccagcacgg cctgcaatca atgccgcgcc
    28981 tgagagtgat gctgaatgaa aaagaatctg cgccgcagct ctgtcaccac tgtgaagatg
    29041 caccctgcgc ggtggtctgc ccggttaacg ccatcacccg cgttgatggg gccgtgcagt
    29101 tgaatgaaag cctgtgcgta agctgcaagc tgtgcggcat cgcctgcccg tttggcgcaa
    29161 ttgaattttc cggcagccgt ccgctggata ttccggcaaa cgccaatacc ccgaaagcgc
    29221 caccggcacc gcctgctccg gcgcgtgtca gcacattgct tgactgggtg ccaggtattc
    29281 gcgcgatcgc cgtcaaatgt gacctttgta gctttgatga acaaggtccg gcctgcgtgc
    29341 ggatgtgccc gactaaagcc ctgcatctgg tggataacac tgatatcgcc cgcgtcagca
    29401 aacgtaagcg tgagctgact tttaacacgg actttggcga tctcaccttg tttcagcagg
    29461 ctcaaagtgg agaggctaaa tgagcgcaat ttccctgatc aatagcggcg tggcgtggtt
    29521 tgtcgccacc gctgttctgg catttctctt ttcttttcaa aaagcgttaa gtggctggat
    29581 agctggaatt ggcggcgcgg ttggtagtct gtatacggca gccgcgggct tcactgtact
    29641 gactggcgcg gttggcgtga gcggtgcgct gtcgctggta agctacgatg tgcaaatctc
    29701 tccgcttaac gcgatttggc tgattacgct cggtctgtgc ggtctgtttg tcagcctcta
    29761 caacattgac tggcatcgcc acgcgcaggt gaagtgcaac ggcttgcaga tcaatatgtt
    29821 gatggctgcc gccgtctgcg ccgtcattgc cagcaacctc ggcatgttcg tggtaatggc
    29881 cgaaatcatg gccctgtgcg cggtgttcct caccagcaac agcaaagagg gcaaactgtg
    29941 gtttgcgctg gggcgtcttg gcactctgct gctggcgatt gcttgctggc tgctgtggca
    30001 gcgttatggc acgctggatc tgcgcctgct ggatatgcgt atgcaacagc tgccgctcgg
    30061 ttccgatatc tggctgctcg gtgtgattgg ctttggcctg ctggccggga ttattccgct
    30121 gcacggctgg gtgccgcagg cacatgctaa cgcctctgcg ccagctgccg cgttgttttc
    30181 tacggtagtc atgaaaattg gcctgctggg cattttaacc ctgtcactgc tgggcggtaa
    30241 tgcaccgctg tggtggggga tcgcgctgct ggtgctcggc atgatcaccg cgttcgtcgg
    30301 tggtctgtat gcgttgatgg aacacaacat ccagcgcctg ctggcttacc acaccctgga
    30361 aaatatcggc attatcctgc tggggctggg cgctggcgta acgggtatcg cgctcgaaca
    30421 accggcactg attgctcttg gcctggtcgg tggtctgtac catctgctta accatagcct
    30481 gttcaaaagc gtactgttcc tcggagcggg gagcgtctgg ttccgtaccg gtcatcgcga
    30541 tatcgaaaaa ctcggtggta ttggcaagaa aatgccggtt atctccatcg ccatgttagt
    30601 cgggctgatg gcaatggctg cgctgccgcc gctgaatggt tttgccgggg aatgggttat
    30661 ctatcaatcc ttcttcaaac tgagcaatag tggcgcgttt gttgcccgtc tgctggggcc
    30721 gctgctcgcc gtgggtctgg caattaccgg tgcgctggcg gtgatgtgta tggcgaaagt
    30781 ctatggcgtc acgttcctcg gcgcgccgcg taccaaagaa gccgaaaacg ccacctgtgc
    30841 accgctcctg atgagcgtaa gcgtagtggc actggcgatt tgctgcgtaa ttggcggtgt
    30901 tgctgcgccg tggctgctgc cgatgctgtc tgctgctgta cctctgccgc tggagcctgc
    30961 taacaccacc gtttctcaac cgatgatcac gttgctgctg attgcctgcc cgctgctgcc
    31021 attcatcatt atggcgattt gcaaaggcga tcgtttgcca tcgcgttccc gcggcgcggc
    31081 ctgggtgtgc ggctacgacc acgaaaaatc aatggtgatt accgctcacg gttttgccat
    31141 gccggtgaaa caggcgcttg cgccagtgct gaaactgcgc aaatggctga atccggtgtc
    31201 gctggtgccg ggctggcagt gcgaaggtag tgcgttgctg ttccgccgga tggcgctggt
    31261 tgaactggcg gtactggtgg tgattattgt ttcacgagga gcctgagaat gagtgtttta
    31321 tatccgttaa ttcaggcgct ggtgttattt gccgttgcgc cgctgctctc cggtattacc
    31381 cgcgtggcgc gcgcccgtct gcataaccgt cgtgggccgg gtgtgttgca ggagtatcgt
    31441 gacattttca aactgctggg ccgccagagc gttggcccgg atgcctccgg ctgggtgttc
    31501 cgcctgacgc cgtatgtgat ggtgggcgtc atgctgacta tcgctactgc gctgccggtg
    31561 gtgaccgtcg gttctccgct gccgcaactg ggtgatttaa tcaccttact gtatctcttt
    31621 gccatcgcgc gtttcttctt tgccatttct ggtctggata ccggtagccc gtttaccgct
    31681 atcggcgcga gccgtgaagc gatgcttggc gtgctggtcg aaccgatgct gctgcttggt
    31741 ctgtgggttg ccgcacaggt tgccggttcc accaacatca gcaacatcac cgacaccgtt
    31801 tatcactggc cgctgagcca gagcatcccg ctggtgctgg cgctttgtgc ctgtgcgttc
    31861 gccactttta tcgaaatggg caaactgccg ttcgacctgg cggaagccga gcaggagttg
    31921 caggaaggtc cgctctccga atacagcggt agcggctttg gcgtcatgaa atggggtatc
    31981 agcctgaaac agctggtggt gttgcagatg ttcgtcgggg tgtttattcc gtggggacaa
    32041 atggaaacct tcaccgccgg tggactgctg ctggcgctgg tgattgccat cgtcaaactg
    32101 gtggtcggcg tcctggttat cgcgctgttc gaaaacagca tggcccgtct gcgtcttgat
    32161 attactccgc gcattacctg ggctgggttt ggctttgcat ttttagcgtt cgtctccttg
    32221 ctggcggcgt gattaaagag agtttgagca tgtctgaaga aaaattaggt caacattatc
    32281 tcgccgcgct gaatgaggca tttccgggcg tcgtgctgga ccacgcctgg cagaccaaag
    32341 atcagctgac tgtcaccgta aaggtgaact acctgccgga agtggtggag tttctttact
    32401 acaaacaggg tggctggctg tcggtgctgt ttggtaacga cgaacgcaaa ctgaatggtc
    32461 attacgccgt ttattacgtg ctgtcgatgg agaagggcac taagtgttgg gtaacggttc
    32521 gcgtcgaagt tgacgccaac aaaccggagt atccgtccgt gacgccgcgc gttccggcgg
    32581 ctgtgtgggg cgagcgcgaa gtgcgcgata tgtacggttt gattccggtt ggtctgccgg
    32641 atgaacgtcg tctggtgctg ccggatgact ggccggatga actttatccg ctgcgtaaag
    32701 acagcatgga ttatcgtcag cgtccggcgc cgaccaccga tgctgaaacc tacgagttca
    32761 tcaatgaact gggtgacaag aaaaacaacg tcgtgccgat tggtccgttg cacgtcactt
    32821 ccgacgaacc gggtcacttc cgtctgttcg tcgatggcga aaatattatc gacgccgact
    32881 accgcctgtt ctacgtccat cgcggcatgg aaaaactggc ggaaacccgt atggggtaca
    32941 acgaagtgac cttcctctct gaccgtgtgt gcggcatctg cggctttgct cacagcaccg
    33001 cctacaccac gtcggtggaa aacgcgatgg gtattcaggt gccagaacgt gcgcagatga
    33061 tccgcgccat tctgctggag gtggaacgcc tgcactcgca tctgctcaac ctcggcctgg
    33121 cctgtcactt tactggcttc gactccggct ttatgcagtt cttccgcgtg cgtgaaacct
    33181 ccatgaaaat ggcagagatc cttaccggtg cgcgtaaaac ctacggcctg aacctgatcg
    33241 gagggattcg tcgcgatctg ctgaaagatg acatgatcca gacccgccag ctggcgcaac
    33301 aaatgcgtcg tgaagtgcag gagctggtgg atgtgctgct gagtacgccg aacatggaac
    33361 agcgcactgt cggcattggt cgtctggacc cggaaatcgc ccgcgatttc agtaacgttg
    33421 gcccgatggt ccgcgccagc ggacacgctc gcgatacccg cgccgatcac ccgtttgttg
    33481 gttatggcct gctgccaatg gaagtccaca gcgagcaggg ctgcgacgtt atttcccgtc
    33541 tgaaagtgcg tatcaacgaa gtctataccg cgctgaacat gatcgactac ggtctggata
    33601 acctgccggg tggtccgctg atggtggaag gctttaccta cattccgcac cgtttcgcgc
    33661 tgggctttgc cgaagcgccg cgcggcgacg atatccactg gagcatgacc ggcgacaacc
    33721 agaagctgta ccgctggcgc tgccgtgccg cgacctacgc gaactggccg accctgcgct
    33781 acatgctgcg tggcaatacc gtttccgatg cgccgctgat tatcggtagc ctggaccctt
    33841 gctactcctg taccgaccgc atgaccgtgg tcgatgtgcg taagaagaag agcaaagtgg
    33901 tgccgtacaa agaactcgag cgttacagca ttgagcgtaa aaactcgccg ctgaaataag
    33961 gaatcgccat gtttaccttt atcaaaaaag tcatcaaaac cggcacggcg acctcgtctt
    34021 atccgctgga gccgattgcg gttgataaaa acttccgtgg taagccagag cagaacccgc
    34081 agcagtgcat cggctgcgcg gcctgcgtca atgcctgccc gtcaaacgcc ttaacggttg
    34141 aaactgacct cgccacagga gagcttgcgt gggagtttaa tctcgggcgc tgcatcttct
    34201 gtggacgctg cgaagaagtc tgcccgacgg cagcgatcaa actgtcgcaa gagtacgaac
    34261 tggcggtgtg gaagaaagaa gatttcctgc aacagtcccg ttttgtgctg tgcagctgtc
    34321 gcgtctgcaa tcgtcctttc gccgtccaga aagagatcga ctacgccatt gcgctgctta
    34381 agcacaatgg tgacagccgc gcggaaaacc accgcgaaag ctttgagact tgcccggaat
    34441 gtaagcgcca gaaatgcctg gtgccgtccg accgtattga actgactcgc catatgaaag
    34501 aggccatctg atgagcaatt tattaggccc ccgtgacgcc aacggcattc cggtcccgat
    34561 gacggtggat gaatccatcg ccagcatgaa ggcgtcgtta ctgaaaaaaa tcaaacgttc
    34621 tgcctacgtt taccgcgtgg actgcggcgg ctgcaacggc tgcgaaatcg aaattttcgc
    34681 cacgctttcg ccgctgtttg atgcagaacg cttcggcatt aaagtcgttc cttcaccgcg
    34741 tcatgcggat attttactgt ttaccggcgc ggtcacccgt gcaatgcgat cccctgcgct
    34801 gcgtgcgtgg cagtccgcgc cggacccgaa aatctgtatc tcctacggtg cctgcggtaa
    34861 cagtggcggg atcttccacg atctctactg cgtgtggggc ggtacggata aaatcgtccc
    34921 ggtagatgtc tacatccctg gttgcccgcc aacgcctgcc gccacgctgt acggctttgc
    34981 aatggcgctc ggcctgctgg agcagaaaat tcacgcccgt ggcccgggtg agcaggatga
    35041 acaaccggcg gaaatcctgc atggcgatat ggtgcagccg ctgcgtgtga aagtggatcg
    35101 cgaagcacgc cgcctggcag gttatcgtta cggtcgtcag attgccgatg acttcctgac
    35161 acagttaggg cagggcgaag aacaggttgc acgctggctg gaagcggaaa acgatccgcg
    35221 tctgaacgag attgtcagcc atctgaatca tgttgtagaa gaggcgcgta tccgatgagt
    35281 gaaaaggtgg tgttcagtca actgagccgt aaatttattg atgagaacga tgccacgccc
    35341 gccgaggcgc agcaggtggt ctattacagc ctggcgattg gtcaccacct tggggttatc
    35401 gattgcctgg aagcggcgct cacctgcccg tgggatgaat atctggcatg gattgccact
    35461 ctggaggcgg gcagcgaagc ccgccgcaaa atggaaggcg tgccgaaata tggtgagatc
    35521 gtcatcgaca ttaaccatgt gccgatgctg gccaacgcat tcgataaagc ccgggcagcg
    35581 caaacttcgc agcagcagga atggagtaca atgctgctaa gtatgctgca tgatattcat
    35641 caggaaaacg ccatctattt gatggtgagg agactgcgtg actgacgttt tactctgtgt
    35701 tggcaatagc atgatgggcg atgatggcgc aggcccgctg ctggcggaaa agtgcgccgc
    35761 cgcgccgaaa ggtaactggg tggtgattga cggcggtagt gcaccggaaa acgacatcgt
    35821 cgccatccgt gaactgcgcc ctacgcgact gctgattgtc gacgccactg atatggggct
    35881 aaaccccggc gagatccgca tcatcgaccc ggatgatatc gccgagatgt ttatgatgac
    35941 tacccataac atgccgttga actaccttat cgaccagttg aaagaagata ttggcgaagt
    36001 gattttcctc ggcatccagc cggatatcgt cggcttttac tacccgatga cccagccgat
    36061 taaagatgcg gttgaaaccg tttatcaacg actggaaggc tgggaaggga atggcggctt
    36121 cgcgcagtta gcggtggaag aagaataatt ctccatcagg gaattcagcg aaatgaatat
    36181 cagaacaggg atgtgcgctt ttttattgag cttagtgctg cctgcacagg ccaccagctt
    36241 taccgaatat ctgccaatga gtgacagcga atacgcgcaa aagcgggcgc tgaaacctct
    36301 cctgaccatg ccttatgatg ccgagcaaac ctggcatttt agaaaagtgg gcgttgctgg
    36361 cgtgacgctt gaaaagatgc caaatgatga tagtgaatgg caattgaacg gtaaagatcg
    36421 cgccggtaaa tcctggtctg tgcctgtggg tatgctggca aacatagcgg gcaaagggca
    36481 actctatcgc gccgatcttg atcgtaacgg cattcaggat ctggttatct ggttaccaag
    36541 taccggtaat ggtctggctc cttatgcaca tttaattctt atgacgttca cgcgcgaagg
    36601 ccgtccctgt gtctttgaac ccaggggatt ctacaccgca agcaaaactg gcgtagatga
    36661 tttgctggat ttgcaaggca atggtcacac gcaattgctg gatatgcagt ttaattctgg
    36721 ttactggata accagtctgt atcaggtaaa agacgccaaa tggcagcgtg ttcacggctg
    36781 gtttggcaag ttaagctatc ccgcgctcac gcgttttact tataccccca accgtaaact
    36841 cgtcctcaaa cccatcgccg ggcgcgaccc gcaaacggaa gatctggcgc agacgcagcg
    36901 ttgcttgata aagggggatg tgcttgaggg gtgaaccaga actgactttt tgcagagtgg
    36961 ctatgaggat tttgggggta attatatcgc gcacaagtgt ttacatattg tgaacttgga
    37021 tttgatgagc tatagtttcc gaacacataa ggatagaaca tcgcagatgt ggctgtttac
    37081 ttactatcag gaagttaaaa cttaaaaatg cacatcattt ctaaagcgcc atttgaagag
    37141 tctgcgcgta aatatcctaa cgacgctctg gctcttcagg cattgtatcg ggttattaag
    37201 gaaacggatt tttctacacc agaagaaatg cgaacggcgt ttcccaacct cgacaatttt
    37261 agatatcgca acaaatggta cgttctggat gtcggcggga acaatcttag ggttattgcc
    37321 tacataaact ttgttaataa gcggttcttt gtgaagcaca taacgaatca cgcagagtat
    37381 gacaaactga cacgttacta cagagagaac aaagaatgac tgctaatgct gcacgcgctg
    37441 ttaaagcaac aagagaactg gtcaatgcag tcccctttct gggaggcagt gactcagaag
    37501 atgattatcg tgaagcactg gaactggtcg aatatttgat tgaagaagat gacaccaatc
    37561 cacttatcga ctttctggcg agccgcattg cagagtatga aaacaacaac gaaaagtttg
    37621 cagagttcga caaagccgtt gcggcaatgc cggttggcgt ggcgttactt cgcacgttga
    37681 ttgatcaaca taatctgacc tatgcggatt tgaagaacga aatcggctct aaatccctgg
    37741 tcagtcagat tttgtctgga caacgatccc tgacgatatc ccacattaag gcactttcag
    37801 cccgatttgg cgttaagccg gaatggtttc tttgattttg actgatgcca aaccccagat
    37861 tgcccgatgc gctgcgctta tcgggccttc ctggttcgtg cgacatgtag gccggataag
    37921 gcgttcacgc cgcatccggc actgttgctt actctaaatc ttcaccatta ctggcaatca
    37981 cttttttata ccaccagaac gatttcttac gtgtgcgcgt cagcgtgccg ttgcctgcgt
    38041 cgtcacggtc tacgtagaca aaaccgtagc gtttgctcat ttcacccgta gaggcggaaa
    38101 ctaaatcaat acagccccat gtggtgtagc ccatcagcgg aatgccatct gcaatcgctt
    38161 cgcccattgc gcggatatgt tcgcgtaaat agctgatgcg atagtcatcg ttaatctcgc
    38221 cattggcagc aagttcatct tttgcgccga ggccgttttc caccagaaac agcggcttct
    38281 gataacggtc gtacatcatg ttcatggtga tacgcagacc gagtggatca ataccccagc
    38341 cccagtcgct cacttgcaga tacgggttac gcagcgattt cactacgttc gccgcactgc
    38401 tattgttggc gttcatctcc gccgaggcgc agcgcgaggc gtaatagctg aaagagacaa
    38461 aatcgacggt gtttttcagg atttcatcat cgcccggtgc tttgtcgatg gttacccctt
    38521 tttcgcggaa tacgcgggca gagtaagccg gatacgcgcc ccgcgcctgc acatcgataa
    38581 aaaacaggtt ttcccggtct ttctccagcg ccgcccagac atcttccggc ttgcaactgt
    38641 aagggtagaa gttaccgccc gccagcatac agcccacctg attttgcggg ttaacctcat
    38701 gggcgatttt ggtggctagc gcactggcaa ccagctggtg atgcgcggcc tgatatttca
    38761 cctgatcctg attttcacct tcttcaaaca ccagacccgc gccggagaac gggctatgca
    38821 acataatgtt gatttcattg aaggtaagcc agtatttcac cagaccatca aatgcttcaa
    38881 agcaggtccg ggcgtagcgg ctgaaaaact ccaccagctt gcggttacgc caggagccat
    38941 attcggtgac cagatgcatc ggcacatcga agtggcacaa cgtgaccagc ggttcgatac
    39001 cgtacttttt acactcttca aagacagaac gataaaaagc aatgccctgc tgattgggcg
    39061 tgatttcatc gccctgcgga aagagacggc tccaggcaat tgaggtacgg aaaaccttga
    39121 atcccatctc tgccatcagg gcgatatctt ctttataacg atgataaaaa tccgtcgcct
    39181 catggctggg ataaaactcg tcatcgcgca actgaaaacg tttttccagc cccagtttca
    39241 ccgccattcg atgctcgccg tgtgggatca tatcgacagt ggtcagccct ttgccacctt
    39301 cacggaacgc accttcagac tggttggcgg caagcgcgcc gccccataaa aaaccttctg
    39361 gaaatactga cattttcatc ctcaattaag acttacttct ttggcgacgg tcggttgtac
    39421 gctctgatgc tttcgcgcct cggcagtcgc ttcctcaaca ggaatatcct cgaagccgag
    39481 caacagtgtg aggataaacg acagcatgac cgccagcgcc atgaccgcga acacccagac
    39541 gatgctcatt ggattcgccg gatcgaagaa ctgcacgctg gtaaatagcc ccggcgctgc
    39601 cattgagtgg ctggcaagcc ccgccatacc ggcaaccgcg ccgcaaataa agccgctgat
    39661 aagactggcg ataagcggac gtttcaggcg gatcgccacg ccgtataacg ccggttcgga
    39721 aatccccgcc ataatggctg atgccgccgc agccagcgcc gtctggcgca gttccgggtt
    39781 tttcgttttc cacgccaccg ccagtgatga accgcccagc gacaggttag cgccgatctc
    39841 tgacggcatg accatccctt ctttgccggt ttcggcaatg gtctgaatga tggttggcgt
    39901 aaagacgcgg tgcatcccgg tcattaccag cagaggccac agcgcgccca taatggcgac
    39961 tgaaagccag cccagataac catgaatggt gtaaaccagc gccgaaatgg cgctaccgat
    40021 ccagatacca atcgggccaa tcagcaggat tgccagcggt gcggcaatca acacaatcag
    40081 cattggcttg aggaagtttt tggtcaccgc cggtgtaatg cgatccaccc agcgttcgat
    40141 atatgacagg caccaggtca tcaccagcgc cgggatcact gtgtaggtgt atttcaccgc
    40201 ggtgaccgga atcagggcaa attcgacatg ttcaccctgg gccgctttcg ccatcagttc
    40261 aataaaactc ggatgtacca gcacacccgc gatggcaatc gccagcgaca tattggtttt
    40321 aaatttgatg gcggcagatg ccgcgaccat cagcggcagg aagaagaaag caccgtcacc
    40381 aatcacattc agaatggtta aggtcggcga tccttttgtc agcacgccgc tcatctcgag
    40441 gatcatcgcc agcagtttga ccatcgatcc gccgataatc gccgggatca gcggtgacat
    40501 ggtgccgatc agcgcatcga ggatccctgc accaatgcga cgcagcgtga gtttgggttt
    40561 acccacgggc tgtgcgggct gcatatctcc cggcagcagg ctgacgactt cctgaaaggc
    40621 ttgtgaaacg gtattgccga taatcacctg acactggttg tcactacgta ccacgccgag
    40681 cacgccgggg atggttttta acgtcgggct gtcgataagc tggtcatctt tgataacaaa
    40741 gcgcaaacgc gtcatacagt gcgtgaccgc cgagatgtta tcaacgccgc ccagtgccgc
    40801 tatcaccgag cgtgccagcg ccgcataatt tttggccatc ggattttatc ctgttatcag
    40861 tagggtagat gtagttgtta tcagtcgacg taacggaaaa atgccgatgc tgaaacggaa
    40921 ttaggaaacc ggtttcacaa aatcatgaat agttgtcact aaataagcaa gagagttaat
    40981 tgttcatttt ttatatttgt gaaaccggtc acctgaatat ttttcccgcc gattgcgcaa
    41041 ggacaatcgg caaacggtgc agtacactgc gttgattgcg tgtaaaggga tgaaaatgat
    41101 gacgacgatg ctggaagtgg cgaagcgcgc cggggtttca aaagcgaccg tttcccgcgt
    41161 gctttcaggt aatggctacg tcagccagga gactaaagat cgcgtgtttc aggcggtaga
    41221 agagagcggt taccgcccaa atttgctggc ccgcaatctg tcggcgaaga gtactcagac
    41281 gctggggctg gtagtgacca acacgcttta tcacggcatt tattttagtg aattactgtt
    41341 tcatgccgcg cgaatggcgg aagagaaagg gcggcagttg ctattggcag atggtaaaca
    41401 cagcgccgaa gaagagcgcc aggcgattca gtatctgctg gatttgcgct gcgacgccat
    41461 tatgatttac ccgcgttttt taagcgtgga tgagattgat gacatcattg acgcgcacag
    41521 tcagccgata atggtgctta atcgccgcct gcgcaaaaac agcagccata gcgtctggtg
    41581 cgatcataaa cagaccagct ttaatgccgt ggcagagttg ataaacgccg gacatcagga
    41641 gattgctttc cttaccggct cgatggattc ccccaccagc gttgaacgtc ttgccgggta
    41701 taaagacgcg ctgtcgcagc atggtattgc gctcaatgaa aaacttatcg ctaacggtaa
    41761 atggacgccc gccagcgggg ccgaaggggt agaaacgttg ctcgaacgtg gggctaaatt
    41821 tagcgcgtta gttgccagta acgacgatat ggcgataggt gcgatcaaag cgttgcacga
    41881 gcgcggcgta gcggtgccag agcaggtgtc ggttatcgga ttcgatgata tcgcaattgc
    41941 cccctacacc gttccggcgc tctccagcgt aaaaattccg gtaacagaga tgattcagga
    42001 aattattgga cggctgattt ttatgctcga tggcggggat ttctcaccgc cgaaaacctt
    42061 cagcggaaaa ctgatccgcc gcgactcact cattgccctt tcgcgataaa cattgtcatc
    42121 acatgtcatc gtcactagtg tcgatgacat cttttttcct gcattacaga aattaatttc
    42181 cttaaataac agcaaattaa aaactggcat gatttgtgaa tgtatcggcg cattaactgt
    42241 cattgctgga gaatttgatg aaccgtttca tcattgctga cgcgagtaaa tgtattggtt
    42301 gccgtacctg tgaagtggcg tgcgtggttt ctcatcagga aaatcaggac tgtgcatcgc
    42361 tgaccccgga aaccttttta ccgcgaatcc atgtcattaa aggtgtgaac atttccacgg
    42421 cgacagtctg ccgtcagtgt gaagatgcac cgtgcgctaa cgtctgcccg aatggtgcta
    42481 tcagccgtga taaagggttt gttcatgtga tgcaggaacg ttgcattggt tgcaaaacct
    42541 gcgtggtggc ttgcccgtat ggcgcgatgg aagtggtggt acgtccggtg atccgcaaca
    42601 gcggcgcggg gctgaatgta cgggctgaca aagctgaagc caataaatgc gacctgtgca
    42661 accatcgcga agacggcccg gcgtgtatgg cggcttgccc gacccatgcg ctgatttgtg
    42721 tcgatcgtaa taaacttgaa caactgagcg cagaaaaacg tcgccgtacg gcgctgatgt
    42781 tctaaaaatc ttatagaact gttctggcat caaaggaatt cccggaaata ttcgtagaat
    42841 acggagctgg cggtatcgcc gacctgagac tgccggatac gacgcagtcg tgtccggcag
    42901 ggcgggttac gcaattcatg atggcgggaa taactcaatg gcaaaaaaca catcttgcgg
    42961 tgtccaactg cgtattcgag gcaaagtgca gggcgtcggt tttcgtccgt ttgtctggca
    43021 actggcacag caattaaatc ttcacggcga tgtctgtaat gacggcgatg gcgtagaagt
    43081 ccggctgctg gaagacccgg aaacgtttct tgttcaattg catcagcact gcccgccgct
    43141 ggcgcgtatt gatagcgtcg agcgtgagcc gtacatctgg tcacaactgc ccaccgagtt
    43201 cactatacgc cagagcacag gcggcaccat gaatacgcaa attgttcccg atgccgctac
    43261 ttgccctgcc tgtcttgccg aaatgaatac cccaggcgaa cggcgttatc gttatccgtt
    43321 tatcaactgt acccactgcg gcccgcgttt caccattatt cgcgccatgc cttacgaccg
    43381 cccgtttacc gtgatggcgg cgtttccgtt gtgtccggct tgtgacaaag agtaccgcga
    43441 cccgctcgat cgtcgcttcc acgcccagcc ggtggcctgc ccggagtgtg gcccacatct
    43501 tgaatgggta agtcatggtg aacatgcaga acaagaggcg gcattacagg cggctatcgc
    43561 acagttaaaa atgggcaaca ttgtcgccat caaagggatt ggcggatttc atcttgcctg
    43621 cgatgcacgt aacagtaacg cggtggcgac acttcgggcg cgcaaacatc gcccggcgaa
    43681 accgctggcg gtcatgttgc cagtggctga cggtttacca gacgctgcgc gccagttgct
    43741 taccacgccc gccgcgccga ttgtgctggc ggataaaaaa tacgttcctg agctttgtga
    43801 tgatatcgcc cctgacctta acgaagtcgg ggtaatgttg cctgcgaacc cgctccagca
    43861 tttgctgtta caggaactgc aatgcccgct ggtgatgacc tccggcaacc tgagcggtaa
    43921 accaccagct atcagcaacg aacaggcgct ggcggatttg cagggcattg ccgacggatt
    43981 cttaatacat aaccgcgaca tcgtgcagcg gatggatgat tcagtggtgc gcgaaagcgg
    44041 cgaaatgctg cgccgttcgc gggggtatgt gccggatgcg ctggctttgc ctccgggctt
    44101 taaaaatgtt ccgcctgtgc tgtgtctcgg cgcggatctg aaaaatacct tctgcctggt
    44161 gcgcggtgag caagcggtgt tgagtcagca tctgggcgat ttaagtgacg atggcatcca
    44221 gatgcagtgg cgcgaagcgt tacgcctgat gcaaaacatc tacgatttta ccccgcaata
    44281 cgttgtgcat gacgcgcatc cgggctatgt ctccagccag tgggcgcgcg aaatgaatct
    44341 gccgacgcaa acggtgctgc atcatcatgc ccacgcagcg gcgtgtctgg cagagcatca
    44401 gtggccgctg gatggcggtg atgtcattgc tttgacgctc gacggtatcg gtatggggga
    44461 gaacggcgct ttgtggggcg gcgagtgcct gcgggtgaac tatcgcgaat gccagcacct
    44521 gggcggcttg cctgcagtgg cgcttccggg tggcgatttg gcggcgaagc agccgtggcg
    44581 aaacctgctg gcgcagtgcc tgcgctttgt gccggagtgg cagaattacc ctgaaacagc
    44641 aagtgtgcaa cagcaaaact ggagcgtgct ggcgcgggcc attgagcgtg gaattaacgc
    44701 gccgctggcg tcatcgtgtg ggcggttgtt cgatgcagtg gcggcggcac tgggctgtgc
    44761 gccagccacg ttaagttatg aaggtgaagc ggcttgtgct ctggaggcgc tcgcagcctc
    44821 atgccacgga gtgacgcatc cggtgacgat gccgctggtg gacaatcaac tggatctcgc
    44881 cgctttctgg cagcagtggc tgaactggca ggcaccggtt aatcaacgcg cgtgggcgtt
    44941 tcatgatgcg ctggcgcagg gttttgccgc gttgatgcgt gagcaggcca cgatgcgtgg
    45001 tatcactacg ctggtattta gcggcggggt tattcataac cgtttgctgc gtgcacgtct
    45061 ggcgcattat ctcgctgatt tcacattgct ctttccacag agtttaccgg cgggtgatgg
    45121 cggtttgtct ctggggcagg gggttattgc tgcggcgcgt tggttagcgg gtgaagtcca
    45181 gaacggataa aacgtgttgc atttggacac cattgccgga tgcggcgtaa acgccttatc
    45241 cggcctacac atgcgtcccc gtaggtcgga taagacgcgc cagcgtcgca tccgacattt
    45301 taggcacagt agcccaccta catcggcaat gttttcaaca atccaaatgc ctctttcatc
    45361 cgatcctcac tgaccacaaa ggcgcgaagc tggtcagcat cgtcaacacc gcgagccacc
    45421 attccctggc gttcggtatt aatttgccag cgtaaatcct gacgctgggt ttcgcctgcc
    45481 agatgcagcg gtaattccgg cgttttgatt ttcacaagca tcgccggtag tttcagcggc
    45541 gtgttattgc cgagaagatt ttttgccagc accatcgcgc taagttgaat cggctgcagg
    45601 aatggcagta cctgaccgtt gatttccgcg caatcgccca gcgcataaat atcgtcatta
    45661 ctggtttgca gataactatc gacgcaaacg ccgcgattaa tcgtcagccc ggcgcgtcgt
    45721 gccagggcgg tttccgggcg cagtccggtg gcggcaatta ccgcatccac ttcgatgcta
    45781 cgctggcggt ccagcgttgc cagaatgcca gaatccgttt tttccagccc ctgtaactga
    45841 gatttcaaca gcagatgaac gcccatctcc gtcaaccgat gctgcaagcg gctgcttact
    45901 tccggtggca ttaacgacgc cagaatactg gcagcgttgt cgattagcgt gaccgctttg
    45961 cctgcacgac aaaaatccat cgccagttcg ctaccaatca aaccaccgcc aacaatcaac
    46021 acgcgtcggg catcccgcag ttgcgtttca caggcgcgat actcttgctg actatttaac
    46081 gtcagcatta actcacgccc aggcacaggc gggacaaagg cactggcacc ggttgccagt
    46141 actagcttgt cgtattgcca ctgattattc tggcttttca ccacacgggc ttcggcatcg
    46201 atatccgtca cccaggtttg tggaaacagg tgcagattaa actgctcggc aaattcaccc
    46261 gccgtctggc gggtaaggtc atcggcacgt tgcccctgac tgataacatg gctgaggtca
    46321 ggtttgttgt actcatccat gctgtcggcg gcaatcaggg ttaatggaat agtggcgtcc
    46381 tgtttgcgaa tatttttcac cagttggcgg gcggcgaagc ccgaaccgat gatcacaatg
    46441 ccgttactca ttttgcctcc gatgccagtt cgtcaaagac gtctttgccg agggagcatt
    46501 ccgggcagag gaagttatcc gggacttcac tccacggcgt tcctggcgca acgtcctgca
    46561 ttggctcgcc ttttgccgga tcgtaaatcc actggcagac gctgcactgc attcgtgggc
    46621 cgaggtcagc cgtcgtcgtg gcagaggttt cttctttaac taccgtattc accgtgctct
    46681 gcggcagcgg cgcgagcgcc cactgacggg cgatttcgcg accgtgttca cggcataact
    46741 ccagagcgtc ctggtctggt cgccatttgg ctttcaggct gagcgacatt tcgaaacccg
    46801 catcctgcag gcgcgtggaa aggcgatcca ccgcaccgcc gctccagccg tgagagccga
    46861 aagcactggc gcgtttgtta cggaagcgta aaccagtcat ctcctccacc agcccggcga
    46921 ttttcggcat catcacgtta ttcatcgtcg aagtgccgac cagcacgcct tttgagcgga
    46981 agacattagt caggatttcg tttttatcgc ttcgggcgac gttgaaaatt ttcaccgcca
    47041 cgcgtgggtc ggtttccgca atcccctggg cgatagcgtc agccatcatg cgggtgttat
    47101 tcgacatggt gtcgtagaaa atggtgattc tgtcttcctg ataatcagcc gcccatttca
    47161 ggtacagctc gacaatttgc gtcgggttat cgcgccatac cacgccgtgg gaagtggcta
    47221 tcatatcgac tggtaagtta aagcccagga tctcggtaat tttcggcgtt accaggcggc
    47281 tgaacggcgt caggatattg gcgtagtaac gctggcactg ctcgaaaagc tccgtctgat
    47341 ccacttcatc gttgaacaga tgctcgtcgc agtagtgctg accgaaagca tcgttactga
    47401 acagcaccgc gtcgcctgtc aggtaagtca tcatgctgtc cggccagtgc agcattggtg
    47461 tttcgacaaa aatgagctgt ttgccgttgc cgatatccag cgtgtcgcca gttttcacca
    47521 cattaaaatt ccactccgga tgatggtgat gaccatttat cgagtcgata gcgttggcag
    47581 tacagtaaat cggcgtatcg ggaatttgcg ccatcagttc ggtcagcgcc ccggcgtggt
    47641 cctcttctgc atggttaatc acgatgtaat cgatatccgc cagatcgatt tcattacgca
    47701 ggttctgcac aaattcgcgg ctgaatttat ggtcgacggt gtcgatcagc acgttttttt
    47761 cttcgcggat gaggtagctg ttgtagctgc tgccgcgcag cgttttatat tccgtgccgt
    47821 gaaaatcacg cacttcccag tcacgttgac ccacccaatg aatgttattt ttcaccacaa
    47881 tagacatagc aacctcaatt tattcagcgt gttctaaaaa gatgtcttgc taattgcaga
    47941 ttgcgtgcca gttttttatc tgattgtttt tactctataa ataaaattat gctcattaat
    48001 gagtagtcaa aatgactatc aatcaaatgg tcaatatgac aatatctata gtcaaattga
    48061 cagtgaggca aagatgagtt tttccgttga tgtgctggcg aatatcgcca tcgaattgca
    48121 gcgtgggatt ggtcatcagg atcgttttca gcgcctgatc accacgctac gtcaggtgct
    48181 ggagtgcgat gcgtctgcgt tgctacgtta cgattcgcgg cagtttattc cgcttgccat
    48241 cgacggtctg gcaaaggatg tactcggtag acgctttgcg ctggaagggc atccacggct
    48301 ggaagcgatt gcccgcgccg gggatgtggt gcgctttccc gcagacagcg aattgcccga
    48361 tccctatgac ggtttgattc ctgggcagga gagtctgaag gttcacgcct gcgttggtct
    48421 gccattgttt gccgggcaaa acctgatcgg cgcactgacg ctcgacggga tgcagcccga
    48481 tcagttcgat gttttcagcg acgaagagct acggctgatt gctgcgctgg cggcgggagc
    48541 gttaagcaat gcgttgctga ttgaacaact ggaaagccag aatatgatgc caggcgatgc
    48601 cacgccgttt gaagcggtga aacagacgca gatgattggc ttgtcccctg gcatgacgca
    48661 actgaaaaaa gagattgaga ttgtggcggc gtccgatctc aacgtcctga tcagcggtga
    48721 gacgggaacc ggtaaggagc tggtggcgaa agcgattcat gaagcctcgc cacgggcggt
    48781 gaatccgctg gtctatctca actgtgccgc actgccggaa agtgtggcgg aaagtgaact
    48841 gttcgggcat gtgaaaggag cgtttactgg cgctatcagt aaccgcagcg ggaagtttga
    48901 aatggcggat aacggcactc tgtttctgga tgagatcggc gagttgtcgt tggcattgca
    48961 ggccaagctg ctgagggtgt tgcagtatgg cgatattcag cgcgttggcg atgaccgcag
    49021 tttgcgggtc gatgtgcgcg tgctggcggc gactaaccgc gacttacgcg aagaggtgct
    49081 ggcagggcga tttcgcgctg acttgtttca tcgcctgagc gtgtttccac tttcggtgcc
    49141 gccgctgcgt gagcggggcg atgatgtcat tctgctggcg gggtatttct gcgagcagtg
    49201 tcgtttgcgg ctggggctct cccgcgtggt attaagtgcc ggagcgcgaa atttactgca
    49261 acactatcgt tttccgggga acgtgcgcga actggaacat gctattcatc gggcggtagt
    49321 gctgtcgaga gccacccgca acggcgatga agtgattctt gaggcgcaac attttgcttt
    49381 tcctgaggtg acgttgccga cgccagaagt ggcggcggtg cccgttgtta agcaaaacct
    49441 gcgtgaagcg acagaagcgt tccagcgtga aactattcgt caggcgctgg cacaaaatca
    49501 tcacaactgg gctgcctgcg cgcggatgct ggaaaccgac gtcgccaacc tgcatcggct
    49561 ggcgaaacgt ctgggaatga aggattaaat aatcccggcc tgatagaaat cctgcaggtt
    49621 aattgcgccg gtgagtttgc cgttttcatc taccaccggt gcggcagtga ttttgcgctt
    49681 catcaggatc tctttggcgt cgatggcgcg actttgcgat tgcaacgtgg tgccgccgac
    49741 cgtcatcgct tcattaactg gcgtggtgag tgcgccgccg ccaaccagcc agcgacgtaa
    49801 atcgccgtcg gtaaagacgc cttgtacctg ttgttgagcg tcacataccg ccaccagccc
    49861 cagaccggtg cggctgagtt ccagcatcgc atccatcacg ctggcggtta acgccacctg
    49921 tgggatggca tcgtcacggc gcatcagatg atgcacttta ttcagcaagc gagcgcccag
    49981 tgccccggct gggtgggagc gggcaaaatc ttcttcatta aatccgcgcg cctgcatgac
    50041 cgccatcgcc agcgcgtcac ccatcatcag ggtattgacg gtgctggagg tcggcgcaag
    50101 gtgcatcggg caggcttcgc gttctacgga gatatccagc accgctttcg ccgccaggcc
    50161 cagcggtgac gtcggtttgc cggtcatcgc cagcagcgcg atagatttat cttccagacg
    50221 cggaataatc agatccagtt ccttcgcgcc accggagtaa gagataaaca gcatcacatc
    50281 gcggctttca atcatcccca gatcgccgtg cagcgcttct gccggatgga caaaaaaagc
    50341 cggtgtgccg gtactggcaa gtgttgcggc gattttctta ccaatgtggc ccgatttgcc
    50401 aattcccgaa accaccactt tgccttcgca gtgcaggatg atattggcgg cgcgaacaaa
    50461 atcatcgccc agacgttccg gtaaacggct tgcttcctgc aactccagca ttaacgtctg
    50521 acgtcccgcg ttcagtagtg cttcactcat tgctctctcc ggttatgatc acatcgatcc
    50581 ctttctcttc cagcgcctga cgaaacgccg gatcgatacc tgcgtcggta atcagcttat
    50641 cgacgctttc aagactgcaa actacgttgg ggcttttacg gccaaacttt gatgagtccg
    50701 ccatcaaaat cacttcgcgc gcggcattgc acattgcctt actgacggta taaacctcgt
    50761 tgaaggtggt tacgcccgca ttgagatcga tgccgtcggt gcccataaac aatttatcga
    50821 aactgaaatg ttcgaaggca ttctctgcca gctgtccgtg aaatgaggcc gattttttac
    50881 gaaacgttcc gcctggcatc aggatagttt gttcgttatc cagttcggat agcgcattga
    50941 cgatatgcag gctgttggtc atcaccgtga tgttattaaa gcgcgagagc aggggaacca
    51001 tctgcaaaac ggtactgcca gcatcaagaa tgatcgaatc gccatcatgg ataaaactaa
    51061 cggcagcttc tgcaatcagc tctttcttgt gggtgttgat gagcgtttta tgatcgatag
    51121 gcggatcgga ttcctcttta ttcaacacca ctccgccata agtacgaatg acggttccgg
    51181 catgttccag aatgaccaga tctttgcgaa tggttgtgcc tgtggtgtca aagtattgcg
    51241 ccaattcttc aaccgagcat ttaccctgct tttgcagata ctccagaatg gcggcctgac
    51301 gctgacgagg tttcatgggc gtgattacct gatttaggtt tcaaaatgat aacttcgcaa
    51361 gcaagtaaca ttcacaacga aattatccac gtttcagttt aagcgccaat gatagagcat
    51421 tctgtgatag tggatcatgg ggaaagatca catcgggctg taaatcaccc gcatgcatac
    51481 cggtaattgc gggaattttt tgcggtcggg cgaagacggt cagtcctttc ataaacaagg
    51541 tgtcgacgat gcgctgctga tcgtccagcg cgatggcgac cacgacccgc ggtttaaagc
    51601 gcccgctgga acggcccacg ccaacccgcc cttgctggca tagtgtgtcg aaggcacggt
    51661 taaaacgaga aatttgccag ccgcctaagg ccagttgcgc acaccaggcg ataacggcga
    51721 cggtgatgag tgcggatacc ataacttttc cttaatgtag tggcggaagt cctgtatgcc
    51781 tgatgagcgt agcgcatcag gcattctata gtctcacgga gatccttaac ggatctccgc
    51841 tgttaatcag aacatcacct gaccgccggt gacattgatc gactgtccgg tgcagtacga
    51901 cgccttagga ctggcgtaga acagcagcat attcagcacg tcctgataat cgcagccgcg
    51961 tttgagcggt actttgtcga tgtaatactg ctcgacttga tccggtttga tacccagctt
    52021 ggtcgcgtat tgtggcaaca gtgactggaa catcggcgat ttcagcaggt taccgagcat
    52081 cagtgaatgc accgtaatgc cgtactccgc cagatccagc gccagtgatt gagtcagccc
    52141 gacgccacca aatttcgctg cgctgtagcc agagttgtgt ttgctgccca ctttgccgga
    52201 tttcgagttg atctgaataa tgcgaccctg aatcccgtcg cggatcatca aacgcgaaaa
    52261 ttcacgggca cacaggaaat aacccaccag attcacttgt agcgaacggt caaaatcgcc
    52321 gagctggaag tcgctgataa aggctgcttt tgctattccg gcgctataga ccagcaaatc
    52381 cacgcgacca aagatttcat ctaccccacg agagagcgcc agaacgcttt gctcgctagt
    52441 ggcgtcagca ccaaaaccgt acgccatact ttcaccatat tcggcgttaa tttcttgtgc
    52501 cacatttgcg gctttgtcgc tctgaatatc gacaaccgcg acgcgatacc cctcggcagc
    52561 cagaccgtgg cacaggaacg cgcctaatgt ttgcccacca ccgatgacaa cggcaacctg
    52621 attcattttt tactccttaa cagattcaaa cttcaaaacc gatcccggcg cgatatcgtc
    52681 gggtacaggg cctgccacat ggacagtgcc cggaaattcc gcttcgttta aaccatcgaa
    52741 gcgcagggtg acatgaccca gttcgcgaag gttgtcttcc gccacgctgc caacggcggt
    52801 caccggatag cgatgctgcc cgagcgtaaa ttgcaaaccg ggatggagtg cacctttcaa
    52861 ctcgccgtgg caatgaatga agcaatactc ttcgaggtcc gctggcgcgc cttcacgaaa
    52921 ggtgatgagc atctggtcgc tgagggcgtc agtggcactc gcgccgatac gggtgatggt
    52981 ggtctgataa ataacggtca tgtttcagcc tctattgata gataaaaccg gagacaaacc
    53041 aggcgatcag tacagtcggt gcgccggtta aaaagcggct caccagtaca gaagggacac
    53101 cgacgcgaac cgtgtcctga cgggcttccg ccagcgacaa accgaccggg atgaagtcgc
    53161 aggccgcctg cgcgttgatg gcaaacagtg ccggtaaagc cagatgcggc ggaatattgc
    53221 cgagaccaat ctgcacgcca atcaatacgc cgataacctg tgcgataact gcgcctgggc
    53281 cgaggaaagg tgaaagcagt gggaaggagc agatgagcgc cagcatgacc agacccagtg
    53341 gatggctcgc cagcggagca agaccgtggg caatccagtc accaaggcca gaagccataa
    53401 tgatgccaat gagcgccgag acgaatgcca taaacggcag aatggttttt aatacagtgt
    53461 cgatggtgtc acgaccagat tgaaacaaca cggcaacggc agaacccatc cccattccca
    53521 ccttcgccag taaaccatcg ctttgttcgg tgattttctt gctggtgtca tagtcacggc
    53581 ccacggaaga gggttgtggt gtcgcatcac ccactacagt gatgttttct tcttttacgc
    53641 cagagacata aatatcttcc acaatgtact gcgccagtgg accggacttg cccgtcgagt
    53701 ggatattaat ggtgggaata cgccgtttcg gatagatgcc gcagcgtaat gtgccgccac
    53761 agtcgattac cgcgacacca atttccgcct ccgcgggttc accttcttta aatccgtcaa
    53821 tagcctgcca gccagtaagt tgtgccagtt tgtcaacaat cgcaggccgg gtaccggcgg
    53881 tgatatagac gatttttttg cccggcgtgg cttccagctc aagcgggccg ccccagccac
    53941 ccgttccttt ttcgatccga atacgcgtca tgatgttgct cctgccaggt gaactttttg
    54001 ttcaagttga atgcccatct ttttctcaaa aacggcggtg gtcagatcgg tcacccagcc
    54061 gcggaagaaa ttggtgacca gaccaaccag cagataactc accgccagtg ggccaagtgg
    54121 caggttcagc gttgtcagac cgctggcaat gccaagataa acaaacagtt cgccagggtt
    54181 gatatggggg aagaggccat tcattgagtg gcagctataa gaggccgccg cgtagtagct
    54241 gggtttgtac ttttccggca taaagcgacc caggcttaag gtcatcggat tgcaaaagac
    54301 aaacgtgcca atgcacggta acagtaggta acgggaaaca gggttaccgg cgcaacgttg
    54361 agcaaaacgt tcaatacgat gctgaccgat aaaattaatc agtgcgttca tgataaccag
    54421 caggctaatc aacagcggaa gaatgccggt caccatcccg gtaaacacct ctccgccctt
    54481 ttggaacagc ccgataaacc actctgcacc atgagtaatg gtttctatca ttgttctctc
    54541 cttcaggatt tattgtttta ttaccaaacg gcaacctaat ct
//
LOCUS       DBANUU010000010        53257 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000010 DBANUU010000000
VERSION     DBANUU010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.010"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..152
                     /locus_tag="ACIE68_000593"
     CDS             <1..152
                     /locus_tag="ACIE68_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFX5906961.1"
                     /translation="VALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKIIGGC
                     GPTAQ"
     gene            447..1784
                     /gene="frlA"
                     /locus_tag="ACIE68_000594"
     CDS             447..1784
                     /gene="frlA"
                     /locus_tag="ACIE68_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFX5906962.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            1805..2827
                     /gene="frlB"
                     /locus_tag="ACIE68_000595"
     CDS             1805..2827
                     /gene="frlB"
                     /locus_tag="ACIE68_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFX5906963.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2877..3707
                     /gene="frlC"
                     /locus_tag="ACIE68_000596"
     CDS             2877..3707
                     /gene="frlC"
                     /locus_tag="ACIE68_000596"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFX5906964.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            3704..4489
                     /gene="frlD"
                     /locus_tag="ACIE68_000597"
     CDS             3704..4489
                     /gene="frlD"
                     /locus_tag="ACIE68_000597"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFX5906965.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            4589..5320
                     /gene="frlR"
                     /locus_tag="ACIE68_000598"
                     /gene_synonym="yhfR"
     CDS             4589..5320
                     /gene="frlR"
                     /locus_tag="ACIE68_000598"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFX5906966.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5472..6557)
                     /gene="yhfS"
                     /locus_tag="ACIE68_000599"
     CDS             complement(5472..6557)
                     /gene="yhfS"
                     /locus_tag="ACIE68_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFX5906967.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDAQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(6569..7873)
                     /gene="yhfT"
                     /locus_tag="ACIE68_000600"
     CDS             complement(6569..7873)
                     /gene="yhfT"
                     /locus_tag="ACIE68_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFX5906968.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(7885..8238)
                     /gene="yhfU"
                     /locus_tag="ACIE68_000601"
     CDS             complement(7885..8238)
                     /gene="yhfU"
                     /locus_tag="ACIE68_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFX5906969.1"
                     /translation="MKKIGVAGLQREQIKKNIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(8249..9127)
                     /gene="php"
                     /locus_tag="ACIE68_000602"
     CDS             complement(8249..9127)
                     /gene="php"
                     /locus_tag="ACIE68_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFX5906970.1"
                     /translation="MSIDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(9124..10350)
                     /gene="yhfW"
                     /locus_tag="ACIE68_000603"
     CDS             complement(9124..10350)
                     /gene="yhfW"
                     /locus_tag="ACIE68_000603"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFX5906971.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIGRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVHTQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(10350..11513)
                     /gene="yhfX"
                     /locus_tag="ACIE68_000604"
     CDS             complement(10350..11513)
                     /gene="yhfX"
                     /locus_tag="ACIE68_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFX5906972.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKIYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(11597..11959)
                     /gene="yhfY"
                     /locus_tag="ACIE68_000605"
     CDS             complement(11597..11959)
                     /gene="yhfY"
                     /locus_tag="ACIE68_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFX5906973.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(11976..12881)
                     /gene="yhfZ"
                     /locus_tag="ACIE68_000606"
     CDS             complement(11976..12881)
                     /gene="yhfZ"
                     /locus_tag="ACIE68_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFX5906974.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(13150..14154)
                     /gene="trpS"
                     /locus_tag="ACIE68_000607"
     CDS             complement(13150..14154)
                     /gene="trpS"
                     /locus_tag="ACIE68_000607"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFX5906975.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(14147..14905)
                     /gene="gph"
                     /locus_tag="ACIE68_000608"
     CDS             complement(14147..14905)
                     /gene="gph"
                     /locus_tag="ACIE68_000608"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFX5906976.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAKRMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(14898..15575)
                     /gene="rpe"
                     /locus_tag="ACIE68_000609"
     CDS             complement(14898..15575)
                     /gene="rpe"
                     /locus_tag="ACIE68_000609"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFX5906977.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALATGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(15593..16429)
                     /gene="dam"
                     /locus_tag="ACIE68_000610"
     CDS             complement(15593..16429)
                     /gene="dam"
                     /locus_tag="ACIE68_000610"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFX5906978.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(16536..17822)
                     /gene="damX"
                     /locus_tag="ACIE68_000611"
     CDS             complement(16536..17822)
                     /gene="damX"
                     /locus_tag="ACIE68_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFX5906979.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRETAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVRTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(17914..19002)
                     /gene="aroB"
                     /locus_tag="ACIE68_000612"
     CDS             complement(17914..19002)
                     /gene="aroB"
                     /locus_tag="ACIE68_000612"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFX5906980.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(19059..19580)
                     /gene="aroK"
                     /locus_tag="ACIE68_000613"
     CDS             complement(19059..19580)
                     /gene="aroK"
                     /locus_tag="ACIE68_000613"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFX5906981.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(19981..21219)
                     /gene="hofQ"
                     /locus_tag="ACIE68_000614"
     CDS             complement(19981..21219)
                     /gene="hofQ"
                     /locus_tag="ACIE68_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFX5906982.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(21131..21535)
                     /gene="hofP"
                     /locus_tag="ACIE68_000615"
     CDS             complement(21131..21535)
                     /gene="hofP"
                     /locus_tag="ACIE68_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFX5906983.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(21525..21965)
                     /gene="hofO"
                     /locus_tag="ACIE68_000616"
     CDS             complement(21525..21965)
                     /gene="hofO"
                     /locus_tag="ACIE68_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFX5906984.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHQQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(21949..22488)
                     /gene="hofN"
                     /locus_tag="ACIE68_000617"
     CDS             complement(21949..22488)
                     /gene="hofN"
                     /locus_tag="ACIE68_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFX5906985.1"
                     /translation="MNLPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLAHSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(22488..23267)
                     /gene="hofM"
                     /locus_tag="ACIE68_000618"
     CDS             complement(22488..23267)
                     /gene="hofM"
                     /locus_tag="ACIE68_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFX5906986.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQQFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            23387..25939
                     /gene="mrcA"
                     /locus_tag="ACIE68_000619"
     CDS             23387..25939
                     /gene="mrcA"
                     /locus_tag="ACIE68_000619"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFX5906987.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(26104..26664)
                     /gene="nudE"
                     /locus_tag="ACIE68_000620"
     CDS             complement(26104..26664)
                     /gene="nudE"
                     /locus_tag="ACIE68_000620"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFX5906988.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            26984..29119
                     /gene="igaA"
                     /locus_tag="ACIE68_000621"
     CDS             26984..29119
                     /gene="igaA"
                     /locus_tag="ACIE68_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFX5906989.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTNIDIYLDRHVVRQGRYLSLHDEVKNFSL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            29184..29852
                     /gene="yrfG"
                     /locus_tag="ACIE68_000622"
     CDS             29184..29852
                     /gene="yrfG"
                     /locus_tag="ACIE68_000622"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFX5906990.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            29863..30264
                     /gene="hslR"
                     /locus_tag="ACIE68_000623"
     CDS             29863..30264
                     /gene="hslR"
                     /locus_tag="ACIE68_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFX5906991.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            30289..31167
                     /gene="hslO"
                     /locus_tag="ACIE68_000624"
     CDS             30289..31167
                     /gene="hslO"
                     /locus_tag="ACIE68_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFX5906992.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(31302..33026)
                     /gene="yhgE"
                     /locus_tag="ACIE68_000625"
     CDS             complement(31302..33026)
                     /gene="yhgE"
                     /locus_tag="ACIE68_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFX5906993.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            33405..35027
                     /gene="pckA"
                     /locus_tag="ACIE68_000626"
     CDS             33405..35027
                     /gene="pckA"
                     /locus_tag="ACIE68_000626"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFX5906994.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(35103..36455)
                     /gene="envZ"
                     /locus_tag="ACIE68_000627"
     CDS             complement(35103..36455)
                     /gene="envZ"
                     /locus_tag="ACIE68_000627"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFX5906995.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(36452..37171)
                     /gene="ompR"
                     /locus_tag="ACIE68_000628"
     CDS             complement(36452..37171)
                     /gene="ompR"
                     /locus_tag="ACIE68_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFX5906996.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            37399..37875
                     /gene="greB"
                     /locus_tag="ACIE68_000629"
     CDS             37399..37875
                     /gene="greB"
                     /locus_tag="ACIE68_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFX5906997.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            37972..40293
                     /gene="yhgF"
                     /locus_tag="ACIE68_000630"
     CDS             37972..40293
                     /gene="yhgF"
                     /locus_tag="ACIE68_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFX5906998.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLTDLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            40731..40958
                     /gene="feoA"
                     /locus_tag="ACIE68_000631"
     CDS             40731..40958
                     /gene="feoA"
                     /locus_tag="ACIE68_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFX5906999.1"
                     /translation="MQYTPDTAWKITGFSHEISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            40975..43296
                     /gene="feoB"
                     /locus_tag="ACIE68_000632"
     CDS             40975..43296
                     /gene="feoB"
                     /locus_tag="ACIE68_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFX5907000.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            43296..43532
                     /gene="feoC"
                     /locus_tag="ACIE68_000633"
     CDS             43296..43532
                     /gene="feoC"
                     /locus_tag="ACIE68_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFX5907001.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            43735..44613
                     /gene="rpnA"
                     /locus_tag="ACIE68_000634"
     CDS             43735..44613
                     /gene="rpnA"
                     /locus_tag="ACIE68_000634"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFX5907002.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(44642..45412)
                     /gene="bioH"
                     /locus_tag="ACIE68_000635"
     CDS             complement(44642..45412)
                     /gene="bioH"
                     /locus_tag="ACIE68_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFX5907003.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            45450..46133
                     /gene="gntX"
                     /locus_tag="ACIE68_000636"
     CDS             45450..46133
                     /gene="gntX"
                     /locus_tag="ACIE68_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFX5907004.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            46192..46767
                     /gene="nfuA"
                     /locus_tag="ACIE68_000637"
     CDS             46192..46767
                     /gene="nfuA"
                     /locus_tag="ACIE68_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFX5907005.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            47128..48444
                     /gene="gntT"
                     /locus_tag="ACIE68_000638"
     CDS             47128..48444
                     /gene="gntT"
                     /locus_tag="ACIE68_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFX5907006.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(48489..50573)
                     /gene="malQ"
                     /locus_tag="ACIE68_000639"
     CDS             complement(48489..50573)
                     /gene="malQ"
                     /locus_tag="ACIE68_000639"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFX5907007.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(50583..52976)
                     /gene="malP"
                     /locus_tag="ACIE68_000640"
     CDS             complement(50583..52976)
                     /gene="malP"
                     /locus_tag="ACIE68_000640"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFX5907008.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        12610 a        14640 c        13486 g        12521 t
ORIGIN      
        1 aagttgcact ggtaggtgca gtactggcta cgttaactgc atgtactggt catattgaaa
       61 accgtgataa gaactgctct tacgactacc tgctgcaccc ggcaatttct atttctaaaa
      121 tcattggcgg ttgcggtcct actgcacagt aagtctctga cagaaatcgg ctaacaccgg
      181 tttaattgcc cgacgccctc tggattttcc gagggcgtat ttgtttctat gtccttcttg
      241 cccccgcccg taaaaatatt ttcactttaa attcaatttg ataaatacat ccattagcca
      301 cagcgcgatc ctgtgcgaaa ttttgtgatc ttcctccaca ttacataaca tcatatgttg
      361 ttatattcat catgcattgt catgttacct tttaaatgac tgcaaactct cccctacaac
      421 gtattggaca agaaaaaggt atcgctatgg gaagccagga actccaacgc aagctcggat
      481 tttgggccgt tcttgcaatc gccgtcggga caaccgtcgg ctccggtatt tttgtatctg
      541 tgggtgaagt ggcaaaagca gcgggcacgc cgtggcttac ggtgctcgcg tttgtcattg
      601 gcgggttaat tgtgatcccg caaatgtgcg tctatgcgga actatccacc gcttatccgg
      661 aaaatggcgc agattatgtt tatctgaaaa atgccggaag ccgaccgctg gctttcctct
      721 ccggctgggc cagcttctgg gccaacgatg cgccgtcatt gtcgattatg gcgctggcga
      781 ttgtcagcaa tcttggcttt ttaacgccta tcgatccgtt gctcggtaaa tttatcgccg
      841 ccggattaat tatcgccttt atgttgctac acctgcgctc cgttgaaggc ggcgcagcgt
      901 ttcagacgct aattaccatc gccaaaatta tcccgttcac tatcgtcatt ggccttggga
      961 tcttctggtt taaagcggag aattttgccg cccctaccac cactgcgatt ggcgcaacgg
     1021 gcagctttat ggcgctgctg gcggggatct ctgccaccag ttggtcgtat accggcatgg
     1081 cctctatctg ttatatgacc ggcgaaatta aaaaccccgg aaaaaccatg ccacgagcgc
     1141 tgattggttc ctgtctgctg gttctggtgc tctacaccct gctggcgctg gtgatttccg
     1201 gcctgatgcc cttcgacaaa ctcgccaatt ctgaaacgcc gatttccgac gccctgacct
     1261 ggatccccgc actcggcagc accgctggga tctttgttgc catcacggcg atgatcgtca
     1321 ttcttggttc gctttccagc tgcgtgatgt accagccgcg gctggaatac gcgatggcga
     1381 aagacaacct gttctttaaa tgcttcggcc atgtgcatcc gaaatacaac acgccggatg
     1441 tctccatcat cctgcaaggg gcgctgggga tcttcttcat cttcgtttcc gatctcacca
     1501 gcctgctggg ttatttcacc ctggtgatgt gtttcaaaaa taccctcacc ttcggctcca
     1561 tcatctggtg tcgtaaacgc gacgattaca aaccgctgtg gcgtactccg gctttcgggc
     1621 tgatgaccac cctcgccatt gcgtcaagcc tcattctggt cgcctcaacc tttgtctggg
     1681 caccgattcc cggccttatc tgcgccgtca tcgttattgc tactggtctg cctgcttacg
     1741 ccttctgggc gaagcgtagc cgccagctca acgctttgtc gtaatgttat ctggagaaaa
     1801 taaaatgttg gatattgata aaagcaccgt ggactttctg gtcaccgaaa atatggttca
     1861 ggaagtggaa aaagttctca gccatgacgt tccgctggtg cacgccatcg tggaagagat
     1921 ggtgaagcgc gacattgatc gtatttattt cgttgcctgc ggctcgccac tcaacgcggc
     1981 gcaaacggcg aaacatctgg cggatcgctt ttccgatctt caggtctacg ccatttccgg
     2041 ctgggagttc tgcgataaca ccccgtatcg cctcgacgat cgttgcgcgg tgattggcgt
     2101 ttctgactac ggtaaaacgg aagaggtaat caaagcgctg gagctgggcc gggcctgcgg
     2161 cgcactcact gcggcgttca ccaaacgcgc ggacagcccg attacctcgg cggcggaatt
     2221 tagcattgat tatcaggccg actgtatctg ggaaattcac ctgctgctct gctacagcgt
     2281 ggtgctggag atgatcaccc gcctcgcgcc gaacgcggaa atcggcaaga tcaaaaacga
     2341 tctcaagcag ttgccgaatg cgctcggtca tctggtacgc acctgggaag aaaaaggccg
     2401 ccagcttggt gaactggcca gccagtggcc gatgatttat accgttgctg cgggtccgct
     2461 gcgtccgctg ggttacaaag aaggcattgt aacgctgatg gaatttacct ggacgcacgg
     2521 ctgcgtgatt gagagcggag agttccgcca tggcccgctg gagattgtcg aaccgggcgt
     2581 tccgttcctg ttcctgctcg gcaatgatga aagtcgccac accaccgaac gcgccattaa
     2641 ctttgttaaa cagcgtactg acaacgtgat cgtcatcgat tacgccgaaa tttcgcaagg
     2701 gctgcacccg tggctggcac cgttcctgat gttcgtgcca atggagtggc tctgctacta
     2761 cctgtctatt tacaaagatc acaacccgga tgaacgccgc tattacggtg gtctggtgga
     2821 atattaatcc ctctcccggc ccggcaacgg gccgaattta cgcaaggagt tacccgatga
     2881 aaacaggtat gtttacctgc ggccaccagc ggctgcctat tgaacacgca tttcgtgatg
     2941 caagcgagct gggttacgac ggcatcgaaa tttggggcgg tcgcccgcac gcgttcgcgc
     3001 cggacttaaa agcgggcggc atcaaacaaa tcaaggcgct ggcgcagacg tatcagatgc
     3061 cgattatcgg ctatacgcca gaaaccaacg gctatccgta taacatgatg ctgggcgatg
     3121 aacatatgcg tcgcgaaagc ctcgacatga tcaagctggc gatggatatg gcaaaagaga
     3181 tgaacgcggg ttatacgctg atttccgcgg cccacgcggg ctatctcacg ccacctaatg
     3241 ttatctgggg acggctggca gagaacctaa gcgaactgtg tgagtacgcg gaaaacatcg
     3301 gcatggatct aatcctcgaa ccgttaacgc cgtatgaatc gaacgtcgtg tgtaatgcta
     3361 atgatgtgct tcatgcgctg gcgctggtgc cttcgccgcg cctgttcagc atggtcgaca
     3421 tttgcgcgcc gtatgtccag gcggaaccgg tgatgagtta tttcgacaaa ctgggcgata
     3481 aattacgtca tctgcatatt gtcgacagcg acggggccag cgacacgcat tacattcctg
     3541 gagaaggcaa aatgccgctg agggaactga tgcgcgatat tatcgaccgg ggctatgagg
     3601 gttactgtac ggtggagctg gtgacgatgt atatgaacga gcccagactc tatgcccgcc
     3661 aggcgctgga acgctttcgc gcgctgctgc cggaggatga gagatgaaaa ccctggcgac
     3721 aatcggcgat aactgcgtcg atatctaccc gcaactgaat aaagcgtttt ctggcggtaa
     3781 tgcggtcaat gtggcggtgt actgcactcg ctacggcata cagccgggat gcattacctg
     3841 ggtgggtgac gatgactacg gcacaaagct gaagcaggat ctcgcccgca tgggcgtcga
     3901 tatcagccat gtccatacga aacacggcgt taccgcacaa actcaggtgg aactgcacga
     3961 caatgatcgc gtttttggcg actacaccga aggcgtgatg gccgactttg ccctgagtga
     4021 agaggattac gcctggctgg cgcagtatga cattgtgcac gcggcaatct ggggacatgc
     4081 ggaagacgca ttcccacagc tgcacgctgc gggcaaactt accgctttcg acttctccga
     4141 caagtgggac agcccgctct ggcagacact ggtgccgcat ctcgattttg cctttgcctc
     4201 cgcaccgcaa gaagacgaaa cgctgcgtct gaagatgaaa gcgattgttg cccgtggcgc
     4261 aggaacagtg attgtcacgc tgggtgaaaa cggcagcatt gcctgggatg gcgcgcagtt
     4321 ctggcgtcag gctcctgaac cggtgacggt tatcgacacc atgggtgccg gagattcgtt
     4381 cattgccgga ttcctttgcg gctggtctgc ggggatgaca ttaccgcagg cgatggcgca
     4441 gggaacggcg tgcgcggcga aaaccattca gtaccacggt gcctggtagg tataacgttg
     4501 gcgtgagcat cttcacgcca acgtgctgtt acttgccgga aaacgaccct ataatccgag
     4561 taattcattc tttatttcag ggtcgattat gtcagctacg gaccgctact ctcatcaact
     4621 cctctacgct accgtccgcc agcgactgct ggatgatatc gcgcaggggg tttaccaggc
     4681 cgggcaacag atccctaccg aaaacgagct ttgtacacaa tataacgtca gccgcattac
     4741 cattcgcaaa gccatcagcg acttagtggc agacggcgta ctgatccgct ggcagggaaa
     4801 aggcaccttt gtacaaagcc agaaagttga aaacgccctg cttactgtca gtggttttac
     4861 cgattttggc gtctcacaag gcaaggcgac gaaagagaaa gtgatcgaac aggaacgggt
     4921 cagcgccgcg ccgttttgcg aaaagctgaa catccccgga aacagcgaag tgttccatct
     4981 ctgccgggtg atgtatctcg ataaagagcc gctgtttatt gatagttcat ggatcccgct
     5041 gtcgcgttat cctgactttg atgagattta cgtcgaagga agctccacct atcagttatt
     5101 tcaggagcgt tttgacacgc gagtggtcag cgacaaaaag accatcgata tctttgccgc
     5161 cacccgcccg caggcaaaat ggctgaaatg cgaactgggc gaaccgttgt ttcgcatcag
     5221 caaaatcgcc tttgatcaga atgacaaacc ggtgcacgtc tccgaactct tctgccgcgc
     5281 caatcgcatc accttaacta ttgataataa aagacattaa ccgtaggccg gataagatgc
     5341 gccagcatcg catccggcga tgctggcgcg ttgaatttta catcccgtac gttcccctca
     5401 ccctaaccct ctccccaaag gggcgagggg accgttcagt acggaattta gcttgacggt
     5461 gctatccagc attaaatact ggcaatactc tcacgcaaaa tccgcagcac cgtctcttca
     5521 ccgctgcgat tcgggttaat gcgaatcgca caatgttctg attgtggatt cgcctggcga
     5581 aacgttccgg aaaggcgata aaagagcggc gggatttcat atttcgactc tgcacccacc
     5641 gggtaaggca aggcaccgcg cttttgcgcc tcttccagca ctctggcggc aatcggctga
     5701 tgaaactcga caatcaacac cttcgactgc gcattagcaa tcaccgcgct tttcacttcc
     5761 ggcaccgcac caccgttaag caaagccagc aaccgttcag atacccctgc ctgcaccgcg
     5821 tgcatcactg gcgcaaacac cagaccacgc aatacttcca gcgcctgtgc gccctggatc
     5881 tggctaccgc cggagtaaag cgtggcgcga atacggttga taacatcagc atcgccgacc
     5941 actgcaccaa caccctctgg cccaaatagc ttgaagcagg aaaatgtcga gacattcgcg
     6001 ccgcattcac agccgattcg cgccaccttc atcaccgcat agttgtcatc ggttaacgct
     6061 ggaacacctg ccgcgcgcaa cgttgccagc acatctgcca gcacgtagct gtcctgcggc
     6121 tgctggcgcg tatgctgcac cagcgccgca tccggttgtt gcgcgtcgac gacctgcttc
     6181 agtgccgaca ggtcattgaa atcaacagta ataagcgtca gccccatctg ctcaataata
     6241 acccgtgtcg tcgggtaaac aggcgcgtca tgcaccagaa gacgctgccc cggtttgagc
     6301 aaagccgcca gcccggcgcg aatcgcgcca gtccccgcgc cctgcaccag cgccgcagcc
     6361 tgtgcgtgaa atgcatcagc cagcacctgt tccacacgct gggtaacgcg cggttgattc
     6421 agtcctggcg ttaagcctaa atcaccgcca gtaagaaact cgctgccggg gaaatggcga
     6481 caaatgctat ccaccagcgc aaactgcttt tgctgcgcct caataatcgt caggctttgc
     6541 agaggaaacg tcttcatagg tgccttcctt aagccggaac gaacaggcca agccagtaaa
     6601 gaacattcag cagaataccg gtgatcatta ctgccacgac cggtgccgcc attttctgta
     6661 ccggacggcc cagggattcg ttgaggaagt aaatggcaac cgcgatagag aatccggtat
     6721 aacccgccat cttaattgct gcgaaaatcg aaccgaccag cagcgccact tccatcagca
     6781 tattcatggc gttacggatg ttatccgacg cattacgcac cgacgggtag cgtcccagcc
     6841 atttgccgat cgaacgaagc agcaagactt ccgccgaaat aaccactgcg cctaataccg
     6901 ctgcaaccat cggattcggc gagagatagc ccaccgcata aacaaaggta aagcccgcaa
     6961 ctgcatacac acccgttgct aaggcggtgg tggcaatcaa cggtacaaaa cccagcccgc
     7021 gcataaattc tgccagtgcc gcctggttga tcagcgtttg cgattgttcc ggcgttacgc
     7081 ctgcggagta cgctttctcc agagtgaaga tcgacacttc actgccggcg aaaatcttca
     7141 tgctggcaac ggcggcaatc aatgctccca cgatggcgat atagggtaag tttttgataa
     7201 tccgcgacgt gcgttcttca aacaccgaaa gcccgctggc atcaatatca ttttcatcac
     7261 gatgacgcag gtcgtgagtt atcgcgatcc ccagcagcat caccatgcca ataaagattt
     7321 cgatggattc agggttaaga tgtgggaaat agcgcacgac taccacacgg gtcatcagaa
     7381 caaccacggc ggcgaccaga ctttgcttcc agccaaactg gtagaaaatc gccaccagcg
     7441 ggaacagtgc aaaagcagaa accaccggcg agcttaattc cccgaggcta cctaatacat
     7501 ccaccggcag cgcggtcagc agctggttta ctggcaacag gcaagtaagg atcaacacgc
     7561 cccagatagc gccaagacca aacgccatca ggctgtttat cgccagtacg ccgaggatat
     7621 cggtaggaag aaagagtaac catgcgttga gcagcccggt tttcagggta aaagagatcc
     7681 ccaccgaggc cacaaaacca atgctcagac caaaagcgat actccccgcc tcgcgacgat
     7741 tcatatagcc ttcaatcagt tgcggcagga tcgggcggat gccgtcatga aaaacagccg
     7801 ccgagcgatg cgccagaagc gatgtcatac ccgtcaggca cgccaccacg ataatctgaa
     7861 tatacagatc catagtcgtg ccttttattt cagatggtta atcagcatcg gaatcgcgtg
     7921 ttcgacgtgc tcaacggaaa ggccaaacgc cactttccct tcagcgatca ttttggcgat
     7981 atgctcgtct ttcgctttaa tgcctggttt ggcaatggtg caacttttgt tatagccaat
     8041 caccgcgatg gcaatcgaca atgccgcgcc cgcaccggta ttacacgcac cgatgtagta
     8101 atccagttgc ccggatttca ctttcattgc cgcttccatg tcgttgtgaa tgaaaacttc
     8161 aaaactgcca ggagccgtcg cttcaatgtt ttttttaatc tgctcacgct gtaagcctgc
     8221 aacgccaatc tttttcatga gtctgtcctt attggaaaaa ttgagaggga ttttcacgta
     8281 acatcacatc cacatcggcc tgactgaatc ctgactggcg caattgcgga ataaaggtgg
     8341 tcaataaata gtcatagcca taaccaccat tggcttttaa atgggagcgg cgcgtaatat
     8401 ccatcgacag catgacgcgg ttcagcaacc cacggtcacg tagcgcgtgg agcatcgcaa
     8461 tgcgcttttc gtccgggtag taactgttct taccaatggt gtcgaactgc acgtacgcgc
     8521 cgagatcgat catcttcaaa atgttgtcga gattgtcttt cagatcgcag tgaccaacgg
     8581 tgacgcgcga aagatcaact ccgtgggctt gcaacagagc cagttgttcc agccccatgg
     8641 tgctgaacga agtatgcgtg gagatcgggc gtccggtctg gttatgcgcc agcgcagcgg
     8701 caataaacac cttctcttcc agcggcgtaa tcttcccttc gctagagccg atctccgcga
     8761 taatcccggc tttcaggtcg gtgccatcga taccctgttc aatttcatcg accatctcct
     8821 gcgccagttc ctgcacgctg cgggtcgcca catgctccgg gaaaaaagcg tcctggtaat
     8881 aaccggtgca ggccaccacg ttgatccccg tctcgcgcat tacatcgagc ataaattgcg
     8941 cattgcgccc catgtaacgg ttggtcatct caatcacatt acgcacgccc cgggtcatca
     9001 ggtcgttcat ctcctggcaa atgaacgcat actgatcaag gcggcagtcc acgttgtttt
     9061 taaagccgga gagatcaata tgcagatgct catgggccag ggtgtaaccc gtcggatcaa
     9121 tactcatagg gtgtctcctg caaaccggag ggaggaaaga aaagagcgac cattttgtgg
     9181 cggtggcgca cggaaaaatt cacacacggt agcccccaca tcagaaagcg tggtgcgtac
     9241 accgagttgc gtgtggacca gcccttgctg ataaaccagc actggcacca cttcacgggt
     9301 atggtggctg tgaccaatgg tcggatcgtt accgtgatcc gccatcacga ccaggcaatc
     9361 atctggctgc atcgcctcaa caagccgggc aaggttacgg tcaacgacct gcaaacgttc
     9421 ggcataacgt gcgacgtctt ctgcatgacc agcgaggtcg gtttcctgga tattgatgca
     9481 aataaacgcc gtcggatgcg tgttaaattc gtcgagggtg atatccataa tccgctggct
     9541 atccaccagg ttttgccagc tcacgccata aggattgctg acgatatctg ccaccttacc
     9601 taccagcacg gttggcacgc ccgcctcata cagtttttgc ggcacctgca ctttttcatc
     9661 aacaccgtag cccatatgca cgacctggaa gccgttgtca taagcgccag aacgcggcgc
     9721 attaatacca ataaagcgcc cttctttgct ttctgcggca tcgagaatac gctgactgtc
     9781 ggttaacaag ccgccaaatg taatgacccg accgacctgt acctgctcac gcacgatacg
     9841 accaattttg atcgcgtcgt caaaagagat cacagagaga ttggcggtaa tgttatagac
     9901 ctggcctaaa tccgcctcga gattatcgcc aatcgcaacc gcctgattga cccacagaaa
     9961 ttgcagatca tcgccacggc gctccacctg ccagccagcg gaaactaatg cctgctcaac
    10021 acggccaatc acatcgcgaa agggcatccg cagcggcggt aacgggcgcg tgcctaaaat
    10081 ttcctgatgc cccataaagg tatcgccacc ttcatgttgc agctctgcca cgccccaggt
    10141 tgcggaatct gacggctgca tatcgcctgg cgcataaccc aatgcgttga ttagccccag
    10201 cttctccagc gttggtagct gcaaatgcgg caactggctc aggatgtgac cacaggtatt
    10261 cgccccggca tcttgcggac gcaccagcgt gacatctttc attgccccta cgccaaagct
    10321 atcaatcact aacaccacaa atcgcgccat tacgccccca gagagtttcc aagactgtca
    10381 taacgaccaa cgattttcgg ttcgccacgg tgaataccgg acaccagcac cacatcgcta
    10441 cgggtgacaa aaatctgcgt gcggaaacag agcaccactg cactgctcac cggatactcg
    10501 cctgccagcg gcagggtgta gtcgatactg ctgtcatcca cagttttgag attggtttca
    10561 gtaatctttt gattttctgg cgtaaacacc agcgcatgtt gcgcatgacc acgacggtag
    10621 taaccgccgc cgtagcagta gctgtcgcca cggaaatgat gggagatttc acttaaccag
    10681 agcatcgcga tacgttcagg ctgatcgccc tgctggtttg ccggaatagt gcccgtcagc
    10741 gcatgaccgg gttcggcatg agtcacaccg tattccgcca gcaatggcag cgaagtgcag
    10801 ctggtcgctg aaggcgcgtt cagttgctca agtgcaatac cagatttcgc cagttgatcc
    10861 cgtgcctgta tcagcgtgtg aagattcggt gtcggcaaaa cttttccggc agcctcatcc
    10921 caaagcaggc aagggaaatg ggtaagtccg gctaaatgca gccccggcag tttcttaatt
    10981 tcagcaacca cctcgtgcag cgaatgctgt acaaaaccgc tctcctgccc cggataaaga
    11041 aaatcatcgt cgctataaat tttgagcagc acaaactgca cacgtccggc cttcaccgct
    11101 gccgcagaaa cttcccgcgc tttgtcgaga gtaaacacgg tgatgacgtc ggtgccctgt
    11161 tcaacggcgt cagcaacctg atgacaaggg atttgtacca gatgcccctg atgcgccacc
    11221 gacaaaccag cgcggcgcat gactcgcgcc tctttgtaat ccaccgccac aatgccgctg
    11281 taacctaatg ccagcaattt ttccgccagc catgggttac ggccaaactg cttggtcatc
    11341 agatacagtt caatgccgta aagccgcgcc gtctcaatca gtcgcttacc gttttccagt
    11401 acctgatcca cgtcgatcac ccagctgtcc ggggcgatct tgccctgctg ccacaaactt
    11461 agtgcggcgg aaatcagcgc cgggttctgg cgtttcaatg cttctacaaa catcttgttg
    11521 cctcgtttct gttccttgaa ttaaatattc atttttttga atatttaggt tgtgcgacgt
    11581 ggatttgtcg ggagcttcaa acctcttcgt tagcagccat ccataatcca taaaggttgg
    11641 ccagcaaata gccttcttca cacgggttaa cttccagtgc gaattccttc aacaacacct
    11701 gatgcaattg cacaacggct tgccagtggc tggattgcgc cagctctgcc agcaattcgt
    11761 tatccagcgg ctctatttct tcaccacggc gactgcgcat cagtgcactc gccatatgtg
    11821 tcatcgccat cgttccttgc tcactgcgca ccggcagatg gcactctgtt tccagtacgt
    11881 tgacgacctg catcatgcct ttgcagatgt ccttatcaat aacgcctgcc tcgcaaagca
    11941 ggttgagtct ggtttccata tcagatgcct ttcgtttaat aactcggttc ctgttcccca
    12001 ctcactaccc gttgttgatg ggcgagcagg gcgtgtttat ctacaacggc acgcagcagt
    12061 tgttgcattg ggtaatcatc gactcgcgtc aggaccacgg cttcggtggc ctgtaaaaat
    12121 cgcggatcgt ctgtgagcgg cgtcgcctct aatcccagca tggtcagttc gttttccgcc
    12181 accacgttcc agatcaccgc atcgacatcg cctttaacaa tgcgttgtaa actctcgtga
    12241 taagagagat cgactcgttc cacatcacta ccgccaaaaa aaacatcggt catgattttc
    12301 tgatccgccg aacggttatc cagccccacg cgcttcacgt ttgcggactc gcctttacgg
    12361 caaatcaact ggtgctcgcc aacgtaggtg tgcggcccca actccagcgc gaggcataac
    12421 cctttttgcg tgagataact ttccgccgcc agtcgggaaa ccaccgccat gtcatacacg
    12481 ccgttaagta aacactccac gcgaatatcc gcgccgcgca tatgcgcata gtaaaaagga
    12541 atgccatcaa actgagcttt cagcccgctc gctaaacctt cgtacaagcg ggtatagggc
    12601 aaaggcatcg cacataccac gttgttgata tccacatgac tcagcaatgc tttgttatcc
    12661 atctcgacca gataactgcc attgcgccca cggcgttcaa tccgtatcgc tccgcttgat
    12721 tccagcgttt tcaacgcggc ctgcgtcagg ccaaccgatg aacggcattc agttgccagc
    12781 tcatctatgg ttttcaacct attaccgcat ttttcaccca acaaataacg ggccagcgtc
    12841 gtgataacga cgccttcttt tttgataaac gttcgacgca taatatattt tcaataaaat
    12901 gaatgtttat atcttcatta aaatgaagat aagtggcaac tgtgagatgt ggaaacggcg
    12961 aggcgcttca cattttttcg gattattgca aatgaggata aaaaaaccgg gtttccccgg
    13021 tttcagagtg atgacgaagt gatttattcg acaatgcgag caaagggtaa ttcccactgg
    13081 cgcttgcctg ctcccctcac cctaaccctc tccccaaagg ggcgagggga ccgtccgagc
    13141 gttttgatat taaggcttcg ccacaaaacc aatcgcttcg tacaccgctt tcagcgtacg
    13201 ggaagcgtgt acactggctt tttctgcgcc gtctttcatc acctgttgca ggaaggcttc
    13261 atcgttgcgg aaacggtgat agcgttcctg caattcagtc agcataccgg aaacggcatc
    13321 agccacttca cctttcagat gaccatacat cttgccttcg aactgttttt ccagttctgg
    13381 gatgctctgg cccgttaccg cagaaaggat atccagcagg ttggaaacgc ccgctttgtt
    13441 ctgcacatcg tagcgaacta ccggcggctc gtcggagtca gtgaccgcgc gtttgatttt
    13501 cttcactacc gatttcggat cttccagcag gccgataacg ttattgcggt tatcgtcaga
    13561 cttggacatc ttcttggtcg gctccagcag cgacattacg cgcgcgccag atttcggaat
    13621 aaacggctcc ggcaccttaa agatctcgcc atacagcgcg ttgaaacgct gggcgatatc
    13681 gcgactcagt tccaggtgct gtttctggtc ttcacccacc ggtaccagat tagtttgata
    13741 cagcaggatg tccgcagcca tcagcaccgg atagtcaaac agaccagcgt tgatgttctc
    13801 ggcataacgc gcagatttat ctttaaactg ggtcatgcgg ctcagttcgc cgaagtaggt
    13861 atagcagttc agtgcccagc ctaactgcgc atgttctggt acgtgggact gaacaaaaat
    13921 ggtgcttttc tcaggatcga taccacaagc cagatacaag gccagcgtat ccagcgtcgc
    13981 tttacgcagc ttctgtgcat cctggcgcac ggtgatcgcg tgttggtcaa cgatacagta
    14041 aatgcaatgg tagtcatcct gcatgtttac ccactgacgc agcgcaccca tgtagttacc
    14101 aatggtcaat tcacctgagg gctgtgcgcc actaaaaacg atgggcttag tcatttttcg
    14161 attcctgatt ttcgctatgc ggaagcccta atgcgggcag aaggtcattt atagactgat
    14221 aaattacatc aggctggctg agatcgatag cctcgccgta gttatatccg taggttaagc
    14281 caactgatgg gcaacctgcc gcttttgccg cctgaatatc attgcgtgag tcgccgacaa
    14341 acagcatctg ttgtggggca attcccatcc gcttagccac cagtaacagc gggtccggat
    14401 gcggtttttt gttttgcaca tcatcgccgc cgataaccac gctaaagtat ttggcaatat
    14461 ctaaggcttc gagcagcggc gcgacgaacg gcgtcggttt gttggtgacc aggcctagcg
    14521 gcaggccttt agcctgcaac gcgcccaacg tatcggcaac gtgcgggaac aaaaacgtcc
    14581 cctcttcggc aacctcgcta tagtagcgat cgaacagttt acgcagaata cgtacctgtt
    14641 cttctgccgg aatgtcgtca tcaacgggcg gtttacccat tgttttacgc tgagtcgcac
    14701 gttcctgacg cgcccaggtc aatgcgcgct ccatcagaac atctgcgccg ttaccaatcc
    14761 aggtaataac gcgttcttca cctgcgatgg gcaactccag cgcatacagc gccatatcta
    14821 ccgcagcagc aagaccggga gcactgtcga ccagcgtacc atcaagatca aaagcgacgc
    14881 cgcgaatatc ttcaaactta ttcatgactt acctttgcca gttcactgcg catttcatca
    14941 atgacttttt tgtagtctgg ctggtcgaag attgccgaac cggcgacgaa catatccgcg
    15001 cccgccgcag cgatttcgcc aatgttgttc accttcacgc caccgtctac ttccagacga
    15061 atgtcaaagc cagactcgtc gatacgacga cgtacttcgc gcagtttatc cagtgtttga
    15121 ggaatgaacg actgaccgcc gaaaccagga ttgacggaca tcagcaggat cacatccagc
    15181 ttatccatca cgtaatccag atagctcagc ggtgtcgccg ggttaaatac cagtcccgct
    15241 ttacagccat tttctttaat cagttgcagc gtgcggtcaa catgctcgga ggcttctgga
    15301 tgaaaggtaa tgatgctggc accagcggca gcgaaatcag gcacaatgcg atcgacaggt
    15361 ttcaccatca ggtgtacgtc gataggggcg gtaatgccgt agttacgcaa ggatttcagc
    15421 accattggcc caatcgtcag attgggaaca tagtggttat ccatgacgtc aaaatgcacg
    15481 acatcagcgc cagttgccag ggcttttgcg gtatcttcac ccaggcgggc aaaatcagcc
    15541 gacagaattg agggggcaat caaatactgt ttcatccgct tctccttgag aattattttt
    15601 tcgcgggtga aacgactcct ggtttgtaca aagccagcag ttcgtccacc tttttacgtg
    15661 tgccgccgtt gctgcttata ctgcgtcgaa ctttgacgac atgcaatttt gcgcgctgat
    15721 accactcacg cgttaacatc gtatcgtgat tggagatcag cacaggaata tggcgatcaa
    15781 ccagaccttc ggcgatctcc gccagatgcg cttgttgttc aagcgtaaaa ctgtttgtgt
    15841 gatacgccgt aaagttggcg gtcgcagaca gcggtgcata aggcggatcg caatagacga
    15901 cggatgcatc atctgcgcgc gccatgctat cggcgtaaga ctcacaatag aaaaaggcat
    15961 tctgcgcttt ttcagcgaag tgatacaact ctgcttccgg gaaatagggt tttttgtagc
    16021 ggccaaacgg cacgttaaac tcaccgcgca gattgtaacg acacaggccg ttgtaaccgt
    16081 agcggttcaa atataaaaac agtaccgccc gacggaacgg atcctggctt ttgttgaact
    16141 cttcgcggaa ctgatagtaa acctcggcgc aatttgtttc gggaacaaac agctcgcgtg
    16201 cggcctgtac gtactcatca gtacgcatct tcacaatgtt atagagactg atcaggtcgc
    16261 tattgatatc ggcaaggata taacgagaaa agtcggtgtt gagaaacacc gacccggcac
    16321 ctacaaaagg ctcaaccaga cattcgccct tgggcaaatg ccgtttaata tcatcaagca
    16381 ggggatactt gccccctgcc cacttcaaaa aagcgcgatt tttcttcatg ctgactaact
    16441 aattacacct tctccggctg tggagaaagc tccgacagca tccagcgctt caggcaactc
    16501 ccgcaacgat aaaccattgc gggagataac cttgattact tcagatcggc ctgtacctga
    16561 cgcagcggtt tcgcccacgg gtttttggcc tgaacatctg ctggcaatgt agataccgct
    16621 tttttcgcct cttctttcga agcgtacacg ccagaaacca ggacatacca cggctgacca
    16681 ttacgcgtcg tttcatagac aacgtagttt ttcagattct ctttcttcgc ccaaccgttc
    16741 aggttgtcgt agttagagga actgctcagc tgcagagtgt aatggctgga cggtgccgat
    16801 ttcaacgaac caacattacc tgcggtcttc cctccagcgg ctggtgttgc cgtggtttgc
    16861 gccggggatg ccgtccgtac tggagccgta gtcgccgtct cttttggtgc tggcgtagaa
    16921 gtcgcagccg gtgccttcgt gctcgccaca ggggcagctg gttcagtacg cttcggcgtc
    16981 tgtgctaccg gcttcggctc cgttttcacg gttgcttgcg gttttttcgg ttcaatcact
    17041 gcctgctggc gagctggacg cgtagtggcc ggacgttcag cggtttgcgt tttcgccgtt
    17101 tcacgggatg cattgccatt gcgaacaggc gcgacagtcg cgggttcggt cggcaatgtg
    17161 gaattgaccg ccacattgtt caactgttgc tgattttgtg gctgggtcag cgcattgttc
    17221 aggtcaccct gcacttcaac acgttgttga ccatccgttg ccgccggggt ttgcccttga
    17281 gtcggcgtag aagagatcgg tggcagagaa acatcctgct gagtattttc cgcagacgtg
    17341 gttcccggcg ctggctgcac gccattcgcc tgatcggtcg cattgccagc aagatcaata
    17401 ctcttctcgc cagacgcggt ttgatcgctg gaagtggtcg agggggcttt tagcgcagaa
    17461 ccgataccga tgatcaacag cagtagaacc agaatgccga cgcccatcat catatactga
    17521 cgagaagcgg gtttactggc tgcttttttg cgcttacgcg gacgacgctc tacgcgctct
    17581 tcatccacgg tttcatcttc ggattcgtca atttcttctt cgatttccgg ttcctcattg
    17641 cgctcttttt gcgcacgagt cggacggcga tcgtcagtgt catcaagttc aatatcatca
    17701 aaattgatct gcggttcgcc acgttcagta cgctcagaac gttcagaaga ttgacgagaa
    17761 cgaccagtac gacgatcgct gggatcgggt ttcagctcgt cttctggttt gaattcatcc
    17821 atttaacacc ccactaaaag gttaatgctt accacgttgc aattaacctg aagctaatag
    17881 accgcttgat aagcggcctg acctttcttg ttgttacgct gattgacaat cggcaatggc
    17941 gttaagaaca agctcgtgcg aaacgccgct gcgaacttca ctcttaccaa ttgccaacgg
    18001 aagaattaag cgcatctctc ccgcaaggac tttcttgtca cgcagcatat gcggtaaata
    18061 cgcctgcgcg gacatttcgc gcggcccatt gaccggtaac ccagcccgcg tgagcagggt
    18121 tataatacgc tgcgtttcgg cagaactaaa ctgcccgaga cgttccgacg tccgcgccgc
    18181 catcaccata cccgcagcga ccgcttcacc atgtaaccaa ttgccatacc ccatttcagc
    18241 ttcaatggca tgaccaaagg tgtgtcccag attcagtaaa gcacgtaacc cggtttcgcg
    18301 ctcgtcggcg gcgacaactt ctgccttcag ttcacaacaa cggcgaatac agtacgccat
    18361 tgccggaccg tccagacgca acaacgcatc cagattctct tccagccagt taaaaaacgc
    18421 accgtcaaga ataatgccgt atttgatgac ttctgccagc cccgacgcta actcacgcgg
    18481 gggaagcgtt ttcagacagt cgagatccac caccactgaa gcaggctggt agaacgcgcc
    18541 aatcatgttt ttaccgaggg gatggttgac cgcagttttg ccgccaacgg aggaatcgac
    18601 ctgcgacagt aacgtcgtcg ggacttgaat gaaacgaaca ccgcgctgat aactcgccgc
    18661 cgcgaagccg gtcagatcgc ccactacgcc gccgccaagc gccaccagcg tagtatcgcg
    18721 accatgcggc ttttgtaaca acgccgtaaa gacggtatcg agtacagcca ggcttttata
    18781 ctgctcgccg tcagggagga taacgctatc gacgttaaca cccgcctgtt caagtacgcc
    18841 gcggacctta tcgagataca gaggagccag ggtttcgttg gtgaccaaca tgacctgctc
    18901 gcccgatttc agcggtaaga atgaagctgg ttcattaaac aaaccagatg cgatggtaat
    18961 tgggtaacta cgttccccga gagtaacgac aatcctctcc ataacgcgac atccacctta
    19021 attactgtac ccgcagacga gtgtatataa agccagaatt agttgctttc cagcatgtga
    19081 ataatctggt ttgcaaccac tttagcgctt tgatcatcag tacgaatggt cacgtcggca
    19141 atctcttcat acagcggatt gcgttcattg gccaacgctt ccagaacttc acgcggcggt
    19201 gtttcaacgt gcagcaacgg gcgtttttta tcacgctgcg tgcgtgcaag ttgcttttcg
    19261 atggtcgttt caagataaac gacaacgcca cgagcggaaa gacggttacg cgtttcacgg
    19321 gatttcacag agccgccgcc agtagccagc acaataccct gtttctcggt caactcattg
    19381 atgacctttt cttcgcgatc gcggaagcct tcttcgcctt ctaaatcgaa aacccagccc
    19441 acatcagctc cggttcgttt ctcaatctct tgatcggaat cgtaaaattc catattgagt
    19501 tgttgagcta actggcgccc aatagtgctt tttccggcac ccataggccc aaccagaaag
    19561 atattgcgtt tctctgccat tttttcggta ctactaagac tattcgttaa tgataaaccc
    19621 gcttcgctca gagagcgccg caggacatga actgaaacct cataagatat tgcgagagtc
    19681 agactgaaaa ttatctcaat actccagcgg gtttggcaac tgaataaatc acccatccct
    19741 ctgcatatct ggtcgctgca agcgcgctgc cttgctacca ccgctctggc gataaatcac
    19801 cgggtaagat tagcgtaaaa aagacagcaa aatgccgcct gaatgataaa tcatcatcat
    19861 ggaacgcagg cagcgaagag caccaactct caaatcggta ctccttgtat gctaaatacc
    19921 tgcgcgcgtc aaatagatga aacacgttca gcataaaaac attaccgctt ttacggctgt
    19981 ttactcactg gaaaccagtc gtggcgtgat aaacaccact aactcgcgtc gttcatcttc
    20041 ttttccgtca tgacgaaata attgcccgaa ccagggaatg tcgccaagca acggtacgct
    20101 atcctgaccc gatttatttt tacgggtaaa aatgccgccc agcgccaacg tttctccgct
    20161 tttgacctcg acctgcgttt cgatctcctg cttatcaatc gccagcactt cgccatcggc
    20221 ctgctgtagc acctgccccg gaacgttctg gctgatgtgt aatttcagcc ggatgcgacc
    20281 tttttgtaac accgtgggcg tgacctccat ccccaggacg gcctctttaa attccaccga
    20341 cgtcgcgcca ctttccccgc tggaaacctg atatggaatt tcgctcccct gtttaatgct
    20401 ggcaggctga agatgtgagg ccagcagacg cggactggcg ataatatcca gctgctgttt
    20461 ttgttcgagc gcggaaagct caagatccag caaacgtccg ttgatgcgcc caatgttaaa
    20521 accgacatgc gttgtcgccg tcgctacgga gaggtcgcta ccaagcgtgg tgacttgccc
    20581 aacgccacca gcgtgttgcg catcggccag cgtccatttc acgcctaact cacgcaaact
    20641 tttttcatta atggtgacaa tatgcgccga cagctcaacc tgcccgaccg gcagatccat
    20701 ttgcgctacc cactgttcaa gcgcgcttaa cgccgttttg ttatctcgta gcaaaaggcg
    20761 attggtgcgt ttatcgacgg tcatactccc tttggcactc agtagcttct cccccgcttt
    20821 cgccagttct cccgcgtcgg cgtattgcag ggttatactg cgattttcca gcggcagatt
    20881 tgcctgcgcc cgcgcctgct ccgcctcctg gcgggcgata ttgttattct gccaggcaat
    20941 ggaatgcact gagagaatgt tgtcttcctg ccgcgttatc agtccggcgc ttttcactac
    21001 agtttgtagt gcctgcttcc agggaacatc tgttagatgt aacgacaccg taccgctgac
    21061 gtctggcgac acgaccaggt tcaacttctc ctgttcagcc agcgcctgca acacctgagc
    21121 taccggaacg tcatccacca tcagcgtcac tttttgcggc tttgccgcct gtacgccggg
    21181 tatcagcatc aacagtagtg cggctatcca ttgcttcatt tgtatctcct tgccgttgcc
    21241 acaaccattg tggcggttcg cagtttgtcc cggtacccag cgttagtacg tctggcgtca
    21301 gctgtaaaat tgtccagccg ttttccagca catcgttttg ctgcacccgt cgccatttct
    21361 tttgcccgtc ttttattaca ccgatgatgc gctcgcctcg ccctaccatc ccctgatagc
    21421 gccactggct aagttcgcta atccggcata gatcttccgg cggtttaaaa gggtcacgca
    21481 taccggttaa aaggcacaat gcaatacctg ccaacaacca gcgtttaacc ctcatgcggc
    21541 gtctccagtt gtagcgtgaa caaaagatca tcaccttcca cgcttaacga aaaacggctc
    21601 acgctgacgt tgcgctctgc cagccgtgta aatgccgacg gcactgcttc ccacagcgtt
    21661 ttcaacgcca actcgcctcc ctgcgcggat ggatgccagg aaaccagttg cgcgccggat
    21721 aactgaaaat ccagtggcga aaagggcagc gttttttcct cgctgaaggg agtggtgtct
    21781 accaggcgat acagtgcggc ccactgctgt tgatgacttg cccgtagtcg aattaatgcg
    21841 tcgcgctctt catggtgtgt cgatgacaga aaaatgagcg taactaacat cagcaaccag
    21901 aatgcccagc aaaactggcg gaggcggggt gatgtggcga accaccagtc aaagaacatg
    21961 ttcatcgcta accttccttg ttaactgata ctcaaattgc cagcgtccct gcgcatcctg
    22021 ctgcgtggcc ccccgctgat tgagatgaaa agaagcatcc tggcgaagtg acgtttctag
    22081 tgcgtttaac gcggtaatgc ttgttgtaag ccccttgatc tccagcgttc cctgctgcca
    22141 gcttatcgtt gtcagccagg cgtgttcagg taaaagagcc gccagtgctt ccagcgcaga
    22201 ttgccagtcg cgggtaaatt gtcgctggcg ctgcctttgc aaacgctgct cacgaatttg
    22261 ttggcgctcc agcaaacgtg gtttcgttat ctgtaagctg tgggcgagtt gttgttcccc
    22321 ctgaagcaaa acggcgtcta cgcgagcttc ggcgctgctt gtcagacgca gtattagcgt
    22381 tatcccgacg gccagcagca gaggcgcaac gaacatcagc aaccagaaac gcagaaaagc
    22441 ggtccggcgt tgctgtcgcc agggcaaaaa attaattggc aggttcatca gtacgccttc
    22501 ccaagcgcca gccccagcgc gatggcaaag tctccacctg gcggcggtag cggcggctga
    22561 cgaacagaaa cggcctccca gggatcaaat ccgccttcgc cacatatcgc gacgctgtcg
    22621 ggatcaacgg ataacgctgc cgccagctcc ttcgcgctag tcatccctac cgccagtttg
    22681 cgcccccagc gatagcgtgt cgcccacagc cactgttcgt tatcacgcca ggccagacat
    22741 tgctgatgag aaggtaaaaa aggcaggaat tgctgtaatg cactggcatc cggggtgatc
    22801 gcactaacat gaacacgcaa cctttctgcc agcgtaagca gcgttgccag ctctttgctt
    22861 tgcgcggcag tcacgttata agcggggctg agtgagtctt cgctataatc gaagcgcagg
    22921 gagtccggat ccatatccag ctcgcgggcc atcgtccctg acagccaggc cgtttgctcc
    22981 cgctcaccaa gggacatcga cgggcgcgga aatgaccgct gtaatgtgcg actggcggga
    23041 aacgccaaca taatgtgatg acgctgcggc agttcgcgac tccaaggtaa caacgtttta
    23101 gccagctgct gcgcatcaac aatccgccca tctttgataa tgtcgttctc cagcggcaac
    23161 cgccaccagc gttgcaaaaa gcattctttt gcgccccgta cgatcgcaac cgctaccgct
    23221 tcttgctgtt gtaaatgcaa accaatttgc cagatcttaa atgccattgt gatgatctcc
    23281 ttatcacccg tcactctgac gggtatatca atgcgtctgg cttgccttta tactaccgcg
    23341 cgtttgttta taaactgccc aaatgaaact aaatgggaaa tttccagtga agttcgtaaa
    23401 gtattttttg atccttgcag tctgttgcat tctgctggga gcaggctcga tttatggcct
    23461 ataccgctac atcgagccac aactgccgga tgtggcgaca ttaaaagatg ttcgcctgca
    23521 aattccgatg cagatttaca gcgccgatgg cgagctgatt gctcaatacg gtgagaaacg
    23581 tcgtattccg gttacgttgg atcaaatccc accggagatg gtgaaagcct ttatcgcgac
    23641 ggaagacagc cgcttctacg agcatcacgg cgttgacccg gtggggatct tccgtgcggc
    23701 aagtgtggcg ctgttctccg gccacgcgtc acaaggggcg agtaccatta cccagcagct
    23761 ggcgagaaat ttcttcctca gtccagaacg cacgctgatg cgtaagatta aggaagtctt
    23821 cctcgcgatt cgcattgaac agttgctgac gaaagacgag atcctcgagc tttatctgaa
    23881 caagatttac ctcggttacc gcgcctatgg tgtcggtgct gcggcacaag tctatttcgg
    23941 taaaacggtc gaccaactga cgctgaacga aatggctgtg atcgccgggc tgccgaaagc
    24001 gccttccacc tttaatccgc tctactcaat ggatcgtgcc gtcgcgcggc gtaacgtcgt
    24061 gctgtcgcgg atgctggatg aagggtatat cacccaacaa cagttcgatc agacacgcac
    24121 tgaggcgatt aacgctaact atcacgcgcc ggagattgct ttttctgcgc cgtacctgag
    24181 cgaaatggtg cgccaggaga tgtataaccg ttatggcgaa agtgcctatg aagacggtta
    24241 tcgcatttac accaccatca cccgcaaagt gcagcaggcc gcacagcagg cggtacgtaa
    24301 taacgtgctg gactacgaca tgcgccacgg ctatcgcggc ccggcaaatg tgctgtggaa
    24361 agtgggcgag tcggcgtggg ataacaacaa gattaccgat acgctgaagg cgctgccgac
    24421 ctatggtccg ctgctgcctg ccgcagtcac cagcgccaat cctcaggaag cgacggcaat
    24481 gctggcggac gggtcgactg tcgcattgag tatggacggc gttcgctggg cgcgtcctta
    24541 ccgttcggat actcagcagg gaccgacgcc gcgtaaagtg accgatgttc tgcaaacggg
    24601 tcagcaaatc tgggttcgtc aggttggcga tgcatggtgg ctggcacaag tgccggaagt
    24661 gaactcggcg ctggtgtcga tcaatccgca aaacggtgcc gttatggcgc tggtcggtgg
    24721 ctttgatttc aatcagagca agtttaaccg cgccacccag gcactgcgtc aggtgggttc
    24781 caacatcaaa ccgttcctct acaccgcggc gatggataaa ggtctgacgc tggcaagtat
    24841 gttgaacgat gtgccaattt ctcgctggga tgcaggtgcc ggttctgact ggcagccgaa
    24901 gaactcacca ccgcagtatg ctggtccaat tcgcttacgt caggggctgg gtcagtcgaa
    24961 aaacgtggtg atggtacgcg caatgcgggc gatgggcgtc gactacgctg cagaatatct
    25021 gcaacgcttc ggcttcccgg cgcaaaacat tgtccacacc gaatcgctgg cgctgggttc
    25081 agcgtccttc accccaatgc aggtggcgcg cggctacgcg gtcatggcga acggtggctt
    25141 cctggtggac ccgtggttta tcagcaaaat tgaaaacgat cagggcggcg tgattttcga
    25201 agcgaaaccg aaagtagcct gcccggaatg cgatattccg gtgatttacg gtgatacgca
    25261 gaaatcgaac gtgctggaaa ataacgatgt tgaagatgtc gctatctccc gcgagcagca
    25321 gaatgtttct gtaccaatgc cgcagctgga gcaggcaaat caggcgttag tggcgaagac
    25381 tggcgcgcag gagtacgcac cgcacgtcat caacactccg ctggcattcc tgattaagag
    25441 tgctttgaac accaatatct ttggtgaacc aggctggcag ggtaccggct ggcgtgcagg
    25501 tcgtgatttg cagcgtcgcg atatcggcgg gaaaaccggt accactaaca gttcgaaaga
    25561 tgcgtggttc tcgggttacg gtccgggcgt tgtgacctcg gtctggattg gctttgatga
    25621 tcaccgtcgt aatctcggtc atacaacggc ttccggagcg attaaagatc agatctcagg
    25681 ttacgaaggc ggtgccaaga gtgcccagcc tgcatgggac gcttatatga aagccgttct
    25741 tgaaggtgtg ccggagcagc cgctgacgcc gcctccgggt attgtgacgg tgaatatcga
    25801 tcgcagcacc gggcagttag ctaatggtgg caacagccgc gaagagtatt tcatcgaagg
    25861 tacgcagccg acacaacagg cggtgcacga ggtgggaacg accattatcg ataatggtga
    25921 ggcacaggaa ttgttctgat taaaaaggcg cttcggcgcc ttttcagttg gctgacaaag
    25981 tgcatttgtt tatgccggat gcgggtgaac gcgttatccg gctaacaaaa tcgtgaaaaa
    26041 tcaatatatt gcaggaaacc cataagcctg ataagcgttg tgcatcaggc aaacttcacg
    26101 catttacact cgcccctgcc ctttcaacca ttcgcgcacg aggaacagcg cgctgacatt
    26161 acgcgcttcg ttgaagtcag ggtcttccag caaatccatc atatgcgcca gcggccagcg
    26221 cacctgtggt agcggctctg gctcatcgcc ttccagtgat tccgggtaga gatcttgcgc
    26281 taccacgata ttcattttgc tggaaaaata agacggtgcc atgctgagct tcttcaaaaa
    26341 agtcagatcg ttcgcaccaa atccaacctc ttcttttagc tcgcggttag cggcttcgta
    26401 gacgctttca cccggatcaa ttaaaccttt cgaaaaacct aattcgtagg attcagttcc
    26461 cactgcgtat tcgcggatca ggatcaggtg atcgtccaca atcggcacaa tcatcactgc
    26521 ttcccggttg gttggacgca ttcgttcata aacacgccgc acgccattgc tgaactccag
    26581 atccacgctc tcgacggtaa acagtcggga acgggctaca gtttcaacat tcagaatggt
    26641 gggtttttgt aatgatttgc tcatcgtggg atctatgctg tgaaatcagc ggttattgtg
    26701 cgatatcgga cacgctttcg gcaatgtgaa ttgcatgtta tttacattta tgtaacttaa
    26761 taaataatcg tcctcaaatc aaattaaaag tcaataggtt gaaataactc caggaatttg
    26821 ctgatattcc gccttcagag ggtttgctat gatcagcggt tactgtgatg tgcttaatga
    26881 tgctcaagtt aaactccacg cttgccgata gccaaccgca gaatcatgta ttgtgtctgg
    26941 tgcgactgac cacgcctgac agactaagta agatggggaa agcatgagca ccattgtgat
    27001 ttttttagct gctttgctgg cctgctcact acttgcggga tggctgataa aagtgcgatc
    27061 cagacggcgt caactgccct ggaccaacgc cttcgcggat gcgcaaacgc gtaaactcac
    27121 acctgaagaa cgtagcgccg ttgaaaatta tcttgagagc ctgacgcagg tattacaggt
    27181 gcctggccca acgggagcta gcgcggcacc gatctctctg gcgctgaatg ccgaaagcaa
    27241 caacgtcatg atgctgacac acgctatcac gcgttacggc atctctaccg acgatccgaa
    27301 taaatggcgt tactacctcg attcggtaga agtccacctg ccccctttct gggaacaata
    27361 catcaacgat gagaataccg ttgaactgat ttataccgat tcgctgccgc tggttatttc
    27421 actcaacggt catacgctgc aggagtacat gcaggaaacg cgcggctatg ccttgcaacc
    27481 tgttccgtcc acgcaggcgt cgattcgcgg ggaagagagt gagcaaatcg agctactcaa
    27541 tattcgcaaa gaaacgcatg aagaatatgc gctgagtcgt ccgcgcgggc tgcgtgaagc
    27601 gttgctgatc gtcgcctcct tcctgatgtt ctttttctgc ctgattaccc cggatgtatt
    27661 tgttccgtgg ctggcaggcg gcgcattact gctgctgggc gcaggtctgt gggggctatt
    27721 cgcgcccccg gcaaaatcct ccctgcgaga aattcattgt ctgcgcggta caccccgtcg
    27781 ttggggattg tttggcgaaa acgatcagga acagatcaac aatatttcac tcggtattat
    27841 cgacctggtc tatcccgcac actggcagcc atacattgct caggatctcg gtcaacaaac
    27901 caatattgat atctatctcg accgccacgt agtgcgtcag gggcgatatc tttcgctgca
    27961 tgatgaagta aaaaactttt cgttacagca ctggctgcgc agtacgatta tcgctgcggg
    28021 ctcgctgctg gtgctgttta tgctgttatt ctggatcccg ctggatatgc cgctgaaatt
    28081 cactctctca tggatgaaag gcgcgcagac cattgaagcc accagcgtaa aacaactggc
    28141 tgacgctggc gtgcgggtag gcgatacatt gcgtattagc ggtacgggaa tgtgtaatat
    28201 tcgcacttcc ggaacctgga gcgcgaaaac caattcacct tttttaccgt ttgactgctc
    28261 gcagatcatc tggaatgacg cccgctcatt accattacca gaatctgaac tggtcaacaa
    28321 agcgacggca ttgactgaag cagttaatcg ccagctgcac cctaaaccgg aagatgaatc
    28381 tcgcgtcagt gcctcattac gttcagcgat tcaaaaatcc ggcatggtat tgcttgatga
    28441 ttttggcgac attgtactga agacagcgga tttatgttct gccaaagatg actgtgtgcg
    28501 actgaaaaat gcgctggtca atctcggcaa cagtaaagac tgggacgcgc tggtaaaacg
    28561 cgccaacgcc gggaagctcg atggcgtgaa tgtgttatta cgcccggtga gtgcggaatc
    28621 gctggataac ctggtggcaa cctccaccgc gccgtttatc acgcatgaaa cggcgcgagc
    28681 ggcacaatca ctaaacagtc cggcccccgg cggattcctg attgtcagcg acgaaggcag
    28741 cgattttgtt gatcagccct ggccttcggc atcactttac gactacccgc cgcaagaaca
    28801 gtggaacgct ttccagaaac tggcacaaat gctgatgcat acgccgttta acgccgaagg
    28861 tatcgtcaca aaaatcttca ctgacgccaa tggtacgcag catattggcc ttcatccgat
    28921 cccggatcgt tccggcctgt ggcgctatct cagcaccaca ttgctgctac tgacgatgct
    28981 gggtagcgcc atttacaatg gcgtacaggc ctggcgtcgt taccagcgtc atcgcactcg
    29041 catgatggag attcaggcct attatgaaag ctgcctgaac ccgcaactga tcaccccttc
    29101 agaaagcctt atcgaataac acgtttgcgc ggcaggttat gctaccctgt cgcgcaaatt
    29161 gcttcactct ggagatttcc ctcatgcata tcaacattgc ctggcaggac gtagataccg
    29221 ttctgctgga tatggacggc acattgctcg acctcgcctt cgataactat ttctggcaaa
    29281 agctggtgcc tgaaacatgg ggcgcgaaaa acggggttac gccacaggaa gcgatggaat
    29341 atatgcgcca gcaatatcac gacgtacagc atacgctaaa ctggtactgt cttgattact
    29401 ggagtgagca actgggtctg gatatctgtg cgatgaccac cgagatggga ccgcgtgccg
    29461 tactgcgtga agataccatt ccgtttcttg aggcactgaa agccagcggt aagcagcgaa
    29521 ttttgctcac caatgcgcat ccgcacaacc tggcggtaaa acttgagcat accggtctgg
    29581 acgcacacct tgatttatta ctttccaccc acacatttgg ttatccgaaa gaggatcagc
    29641 ggttatggca tgcggtggcc gaagctacgg gtctgaaagc tgaaagaacg ctgtttattg
    29701 atgacagcga agcgattctc gatgctgccg cgcaatttgg tattcgttac tgcctcggcg
    29761 tgactaatcc tgattccggg attgccgaga aacagtatca acgccatccg tcactgaatg
    29821 actaccgccg cctgatcccc tcgctaatgt gaaggagacg ccatgaaaga aaaacctgct
    29881 gttgaggttc gactggataa atggctatgg gctgcccgtt tttataaaac ccgcgcgctg
    29941 gcccgtgaaa tgattgaagg cggtaaggtg cattacaacg ggcagcgcag caagccgagc
    30001 aaaatcgtcg agctgaatgc cacgctcact ctgcgccagg gaaatgacga acgcacggtg
    30061 attgtaaagg cgattactga acagcgtcgc cccgccagcg aggcagcctt gctgtatgaa
    30121 gagactgcgg aaagtgtaga gaaacgcgaa aaaatggcga tggcacgtaa acttaatgcc
    30181 ttaaccatgc cgcacccgga ccgacgcccg gacaaaaaag agcgccgcga cctgttacga
    30241 tttaaacacg gcgacagtga ataactgtca cctgcaagag agatgattat gccgcaacat
    30301 gaccaattac atcgctatct gtttgaaaat tttgccgttc gcggcgaact ggtaaccgtt
    30361 tcggaaaccc tgcaacagat ccttgagaac cacgattatc cgcagcccgt taaaaacgtg
    30421 ctggcagaac tgctggttgc gaccagcctg ttaaccgcta cgctgaagtt tgatggtgat
    30481 atcaccgtac agctgcaagg cgacggtccg atgaatctgg cggttattaa cggtaacaat
    30541 aaccagcaaa tgcgcggtgt ggcgcgcgtg cagggcgaaa ttccagaaaa tgccgacctg
    30601 aaaacgctgg tcggcaatgg ttacgtggtg atcaccatta ccccgagcga aggcgaacgc
    30661 tatcagggcg tggttggtct ggaaggtgat accctggcgg cctgcctgga agattacttt
    30721 atgcgttctg aacagctgcc gacgcgcctg tttattcgca ccggcgacgt agacggcaaa
    30781 ccggctgcag gcggtatgtt gttgcaggta atgcctgcgc aaaatgccca gcaggacgac
    30841 tttgaccacc tggcgacgct aaccgaaacc atcaaaaccg aagaactgct gaccttaccg
    30901 gcaaacgaag tgttgtggcg tttgtatcac gaagaagagg tgacggttta cgatccgcag
    30961 gatgtggagt tcaaatgcac ctgctcgcgt gaacgttgcg ccgatgcgct gaaaacgctg
    31021 cctgatgaag aagttgatag catcctggcg gaagatggcg aaattgacat gcattgtgat
    31081 tactgcggta accactatct gttcaatgcg atggatattg ctgaaatccg caacaacgcg
    31141 tctccggcag atccgcaagt tcattaatga gtatgtccgg cagagagggc tctgccggat
    31201 tttacgacgt aatggcctga tgtgctgcgc ttaccaggcc tgtgaaataa atcgcaaatt
    31261 attgttggtc agatacagcg tcgtatccgt taaaagagtt gtcacttact taagtttatt
    31321 tccagcgttt caccatctac cgtcagatcg cgccacgctt tcggcctcgt ccccagtttt
    31381 ccatccttcg cagctgtgag taatttttct ttcgttattt cgttcggaag taaactcata
    31441 tcaagcgcgt cccattcttt tctgtctgag tcaaagccat agacaatgac tctgtcatca
    31501 ttaaaagcaa acaggatccg ctccgcctga ccatcactat tcagatcttg ctcgaccagg
    31561 acgcaggcgt ctttttcaat acaggtcatc acgttatagc gatcctggat taaggccgac
    31621 cagaatgtcg catccggttt aacagaacca ggggcaatta acacattttc ggctaatact
    31681 ttttccgata cctgttcctg aagatgttgc tctccatcta acgccatcag cagatcccgc
    31741 gcgcgtttcg ggtctttcat aaacccggca tcgcttttca gcgactcaag cgccgcacgt
    31801 ccatagcgac cgctctgttc gagcatgtag atagttacct ggtctggcgt gtttttgccg
    31861 ctctgataac gcgccatatg actgttcacg ctaatacgca tactgtccag caccggcgaa
    31921 ttaagaagca ccagtatcac caacaccaat aacgaaaccg caaggttcac tttgccctga
    31981 agaacaaggg gattttgccc attacgccag acgatactga caaaataccc cagcgaccac
    32041 accagtaaca ccagcaccgc cagcgcgccc tgcaagcggt cgacagtcca gccgtactga
    32101 gcgacccgca gccataacgc ccaggcggcg acgaacacat acagcggagc aaccagcaaa
    32161 gcggttttaa tcaggcaacg caacggcccc gtccagggaa gtgacgcttt ttgcggatcg
    32221 cggacaatag ccattaagat caattgcaaa aaggccagcg ttaacagcaa cccggcggcg
    32281 gagatgtggc gagaaatcgc gctcaggccc gtaaacggca gagtgatgat aaacatcagg
    32341 gttagcaatg atactaacgg cagcaacccc gtggcgatta acgtgaacaa cttttgaata
    32401 gagtcgatta aacgtgactg tgtgcgcgcg agaatcaccg ccagtgcggt aaccagacct
    32461 aatgtaagat acataaacca gtcggttgca aaaaagagcg tattgaaaaa cttgatgcca
    32521 acaagtttaa acaactcacc ccacagtaaa agcaccagcc acgtcaggcc attggcgaga
    32581 aaaatcacca gtaatataag tacgttatgc catactgact ggtaaaagta gctatagcgg
    32641 ctactgccat tgcggatacg aaggctttgt tgtatccacg gtagcaacaa cattgccatc
    32701 aacagcagat agcagccaaa atcccaaatc gccttttcag ctctccaggg gttcatgcca
    32761 tcagtttgcc acttcagcca accgctcatc cctgtcgtgg caataaacac cagcgccagc
    32821 catccccaaa ggcgcttctg tttaaaagag atcacggaga agagtaaaac ggatgaaaac
    32881 gcaacggtcg caggcacgcc ataaacaatc caactgtgat tttttccggc aagccaggcg
    32941 atcaacaggt agcaaaccag cccctgaagt aatccggtag caatcatccc ccagcgtgtt
    33001 gccggtgaaa gttcaacgtt atccatgttg gttatccaga atcaaaaggt gggttaatta
    33061 tcgcatccgg gcagtagtat tttgcttttt tcagaaaata atcaaaaaaa gttagcgtgg
    33121 tgaatcgata ctttaccgat tgaatttgca tcaatttcat taaggaatgc gattccactc
    33181 acaatattcc cgccatataa accaagattt aaccttttga gaacattttc cacacctaaa
    33241 atgctatttc tgcgataata gcaaccgttt cgtgacagga atcacggagt tttttgtcaa
    33301 atatgaattt ctccagatac gtaaatctat gagccttgtc gcggttaaca cccccaaaaa
    33361 gactttacta ttcaggcaat acatattggc taaggagcag tgaaatgcgc gttaacaatg
    33421 gtttgacccc gcaagaactc gaggcttatg gtatcagtga cgtacatgat atcgtttaca
    33481 acccaagcta cgacctgctg tatcaggaag agctcgatcc gagcctgaca ggttatgagc
    33541 gcggggtgtt aactaatctg ggtgccgttg ccgtcgatac cgggatcttc accggtcgtt
    33601 caccaaaaga taagtatatc gtccgtgacg ataccactcg cgatactttc tggtgggcag
    33661 acaaaggcaa aggtaagaac gacaacaaac ctctctctcc ggaaacctgg cagcatctga
    33721 aaggcctggt gaccaggcag ctttccggca aacgtctgtt cgttgtcgac gctttctgtg
    33781 gtgcgaaccc ggatactcgt ctttccgtcc gtttcatcac cgaagtggcc tggcaggcgc
    33841 attttgtcaa aaacatgttt attcgcccga gcgatgaaga gctggcaggt ttcaaaccag
    33901 actttatcgt tatgaacggc gcgaagtgca ctaacccgca gtggaaagaa cagggtctga
    33961 actccgaaaa cttcgtggcg tttaacctga ccgagcgcat gcagctgatt ggcggcacct
    34021 ggtacggcgg cgaaatgaag aaagggatgt tctcgatgat gaactacctg ctgccgctga
    34081 aaggtatcgc ttctatgcac tgctccgcca acgttggtga gaaaggcgat gttgcggtgt
    34141 tcttcggcct ttccggcacc ggtaaaacca ccctttccac cgacccgaaa cgtcgcctga
    34201 ttggtgatga cgagcacggc tgggacgatg acggcgtgtt taacttcgaa ggcggctgct
    34261 acgcaaaaac tatcaagctg tcgaaagaag cggaacctga aatctacaac gctatccgtc
    34321 gcgatgcgct gctggaaaac gtcaccgtgc gtgaagatgg cactatcgac tttgatgatg
    34381 gttcaaaaac cgagaacacc cgcgtttctt atccgatcta tcacatcgat aacattgtta
    34441 agccggtttc caaagcgggc cacgcgacta aggttatctt cctgactgct gatgctttcg
    34501 gcgtgttgcc gccggtttct cgcctgactg ccgatcaaac ccagtatcac ttcctctctg
    34561 gcttcaccgc caaactggcc ggtactgagc gtggcatcac cgaaccgacg ccaaccttct
    34621 ccgcttgctt cggcgcggcg ttcctgtcgc tgcacccgac tcagtacgca gaagtgctgg
    34681 tgaaacgtat gcaggcggcg ggcgcgcagg cttatctggt taacactggc tggaacggca
    34741 ctggcaaacg tatctcgatt aaagataccc gcgccattat cgacgccatc ctcaacggtt
    34801 cgctggataa tgcagaaacc ttcactctgc cgatgtttaa cctggcgatc ccaaccgaac
    34861 tgccgggcgt agacacgaag attctcgatc cgcgtaacac ctacgcttct ccggaacagt
    34921 ggcaggaaaa agccgaaacc ctggcgaaac tgtttatcga caacttcgat aaatacaccg
    34981 acacccctgc gggtgccgcg ctggtagcgg ctggtccgaa actgtaatga tttgaagctg
    35041 gagaatatct atccagtatc ttatagaaag caaaacggga ggcaccttcg cctcccgttt
    35101 atttaccctt cttttgtcgt gccctgcgcc cgcgttaccg gcactggcag ccaggcgcga
    35161 atggaaagcc cgccccgctc gctggtgcca agctccagca tcccgttatg gttatccacg
    35221 atacgctgca caattgccag ccctaatccc gtgccgctaa tggtgcgcgc actgtcgccg
    35281 cggacaaacg gctggaacag gtgcttacgt tgttccggcg caattcccgg accgtcatct
    35341 tccacctgga accaggcgcg attcggctcc gttccgctgc tgactttgat ccagccattg
    35401 ccataacggg cggcgttgac caccatattc gccaccgcgc gtttgatcga cagcgggtgc
    35461 attttcactt caatgctgcc ggggtaaagc gcggtttcaa tttcccgctc atagccactt
    35521 tcggcagcaa tcacctcacc gagtactgca ttaagatccg ccatttccat cggcatctcc
    35581 tgcccggtgc gcaggtagtc gataaactgc tcaatgatgg cgttgcactc ttcgatatct
    35641 ttattgatcg attctgccag atagccatcc tgctcgctca tcatctcagt cgccaggcga
    35701 atacgcgtca gcggcgtgcg caagtcgtga cttacccccg ccatcagcag cgtgcggtca
    35761 tccgccagtt gcttaacacc agccgccata tggttaaagg cacgggtaac ggaacgcacc
    35821 tccgaagcgc catattcacg cagcggcggc ggaataatcc ctttaccaac ctgcaaggct
    35881 gcgtgttcga gatcgaccaa cggtcggttc tggatacgaa taaacagcca cgccccgcct
    35941 atcgccaata gcataatcgc cagcgtatag cggaacagcg gagagaaatc gccctgatga
    36001 atttcggtca gcggcacgcg tacccagata ttgggcgaca gccaggtttt cagccagacg
    36061 acaggcgaac ttttgttgac ctcaacgcgc acttccgtcg ggccgcccag ttgctgtgcc
    36121 atctgatggc ttaagaattc atagtgttgc gcccaacgca gacctgcctc ttcggcagcc
    36181 tcgttggagt agagagagat ccccagctca cggtagatct cccgacggaa agcgggaggc
    36241 acaaccaact gcgtgccgtc ctccagttgc agtttgtcgg tcatcaacat acgcacttcg
    36301 tacgcgagga ctttattaaa ctgctggagg ctcggcaaaa tcgcgaagtt cagcaccacc
    36361 agataagtcg tcaccaggct ggcgaacagc aaggtgacga tgagcaataa cgtacgggca
    36421 aatgaacttc gtggcgagaa gcgcaatcgc ctcatgcttt agagccgtcc ggtacaaaga
    36481 cgtagcccag gccccaaacg gtctgaatgt aacgcggatg cgctggatct tcttctacca
    36541 tgcggcgcag acgcgaaatc tgcacgtcga tggagcgttc cattgcggaa tattcacgac
    36601 cacgggcaag gttcatcagc ttatcgcggg agagcggctc acgcggatgg ctgaccagtg
    36661 ccttcagtac cgcaaactca ccgctggtga gcggcatcgg ctcatcttcg cggaacattt
    36721 cgcgcgtacc gaggttaagt ttgaacttac cgaaagcaat taccgcctct tcctgtgacg
    36781 gtgcgcctgg cagttcgttc gcctgacgac gcagcaccgc acgaatacgg gccagcagtt
    36841 cacgcgggtt aaacggtttt ggaatgtagt cgtcagcgcc aatctccagg cctacgatac
    36901 ggtccacttc ttcccctttc gccgtcacca taatgatcgg catcgggttg ctctgactac
    36961 gaagacgtcg gcaaatcgac aagccatctt caccaggtaa cattaaatcc agtaccataa
    37021 gatggaaaga ttcacgagtc agcaggcgat ccatctgttc tgcattagcg acgcttcgaa
    37081 cctggaagcc ttgttcggtg agataacgtt ccagcagcgc acgcaggcgc atgtcgtcat
    37141 cgaccaccag aatcttgtag ttctcttgca ttgtttgtac tcccaaaggt tcgcaacaat
    37201 ttgtaagcgt gtattcttaa aaaagctcac gttcgtcacc agctaaatct ggtatgaatt
    37261 tcagcctaaa ttgttacaaa gcatattaaa cagcagctta agtatacaat ttattcggcg
    37321 aaacattatt gattctgttg atatgatcac gttataccca atgtgcgcat tatcaaacag
    37381 acaaagggaa tcaacgagat gaaaacgccc ctggttaccc gggaagggta tgaaaaactc
    37441 aaacaagagc ttaattatct ctggcgtgaa gaacgcccgg aggtcacaaa aaaggtgacc
    37501 tgggccgcaa gtctgggcga ccgcagcgaa aatgctgact atcagtataa taaaaagcgt
    37561 ctgcgtgaaa tcgaccgtcg cgtgcgctat ctcactaaat gcatggaaaa tctcaaaatc
    37621 gtcgattact cccctcagca ggaaggcaaa gtcttttttg gcgcgtgggt ggagattgaa
    37681 aacgacgatg gcgtgactca ccgtttccgt attgtcggct acgatgaaat ttttggccgt
    37741 aaagattaca tctctatcga ttccccgatg gcccgcgcat tgctgaaaaa agaagtcggc
    37801 gatctggcgg tggtgaatac ccctgccggg gaagcgagct ggtatgttaa tgctatcgag
    37861 tacgtgaaac cgtaaggaag agtcttaacc tcctgccgat ggctggcatt tttgccagcc
    37921 agtccgtata actatcccct gatttttgat ccgaaaagat gaactcaaac catgatgaat
    37981 gattcgttct gccgcattat tgcgggtgaa attcaggcgc gcccggaaca ggttgacgct
    38041 gccgttcgcc tgcttgacga agggaatacc gtgccgttta tcgcacgtta tcgtaaggaa
    38101 atcaccggcg gtctggatga cacgcaactg cgtaatctgg aaacgcgtct gagctatctg
    38161 cgcgagctgg aagagagacg tcaggcgatc ctcaagtcca tttccgagca aggcaaactc
    38221 accgatgatc tggcgaaggc catcaacgcc accctaagca aaaccgaact cgaagacctc
    38281 tacctgccct acaaacctaa acgccgcacc cgcgggcaaa tcgccattga agcagggctt
    38341 gagccgttga ctgacctgct gtggagcgat ccgtcacaca cgccagaagt cgccgctgca
    38401 caatatgttg atgccgataa aggcgtggca gataccaaag ccgcgctgga tggcgcgcgc
    38461 tatatcctga tggaacggtt tgccgaagat gccgcgctgc tggcgaaagt gcgtgattat
    38521 ctgtggaaga acgcgcattt ggtttctacg gtggtgagcg gtaaagaaga ggaaggggcg
    38581 aaattccgcg actatttcga tcatcacgaa ccgttgtcca cggtgccttc tcaccgcgcg
    38641 ctggcgatgt tccgtgggcg taacgaaggc gtactccagc tttcgctgaa tgccgatccg
    38701 cagttcgatg agccgcccaa agagagctat tgcgagcaaa tcatcatgga tcaccttggc
    38761 ctgcgcctga acaatgcccc ggcggatagc tggcgcaaag gcgtagtgag ctggacctgg
    38821 cgcatcaagg tgctgatgca tctggaaacc gaactgatgg gtaccgtgcg cgaacgcgcg
    38881 gaagatgaag caatcaacgt ctttgcccgt aacctgcacg atctgctgat ggcggcccct
    38941 gccggactgc gtgcaacgat gggcctcgat ccgggtctgc gtactggggt aaaagtggcg
    39001 gtggtcgatg ccactggcaa actggtggcg accgatacca tttacccgca caccggacaa
    39061 gccgcaaaag cagcgatgac cgttgctgcc ttgtgtgaaa aacataacgt tgaactggtg
    39121 gcgatcggca acggtacagc ttcccgcgaa actgaacgtt tctatctcga cgtgcagaag
    39181 cagttcccga aagtgaccgc acagaaagtg atcgtcagcg aagctggcgc gtcggtttac
    39241 tcagcttccg agctggcagc gcaggagttc ccggatctcg acgtttcgct gcgtggtgcg
    39301 gtatctatcg cccgccgttt gcaggatccg ctggcggagc tggtgaaaat cgatccgaaa
    39361 tctatcggcg taggtcagta tcagcatgac gtcagccaga cgcaactggc ccgcaaactg
    39421 gacgcagtag tagaagactg cgtaaacgcc gttggcgtcg atctcaacac tgcttctgtt
    39481 ccgctgttaa cccgcgtggc gggcctgacg cgcatgatgg cgcaaaacat cgtggcctgg
    39541 cgcgatgaga acggtcagtt ccagaaccgt cagcaactgc tgaaagtgag ccgtctgggg
    39601 ccgaaagcct tcgagcagtg cgcgggcttc ttgcgcatta accatggtga taacccgctg
    39661 gacgcgtcta ccgtccaccc ggaagcctat ccggtggtgg aacgcattct ggcagcaaca
    39721 cagcaggcac tgaaagatct gatgggtaac agcagcgaac tgcgtaacct gaaagcgtct
    39781 gactttactg atgaaaaatt cggtgtgccg acagtaactg acatcatcaa agagctggaa
    39841 aaaccgggtc gcgatccgcg tccggaattt aaaaccgctc agtttgccga tggcgtcgag
    39901 acaatgaacg acctgcaacc gggtatgatc ctcgaaggtg cggtgaccaa cgtcaccaac
    39961 tttggcgcgt ttgtcgatat cggcgtgcat caggacggcc tggttcacat ctcttcattg
    40021 tcgaacaagt ttgtagaaga tccgcatacc gtggtgaaag cgggcgacat tgtgaaggtg
    40081 aaagtgctgg aagtggatct tcagcgtaaa cgtatcgccc tgaccatgcg tctggacgag
    40141 cagcctggcg aaaccaacgc ccgtcgcggc ggcggtaatg aacgcccgca aaacaaccgc
    40201 ccggcagcca aaccgcgcgg acgtgaagcg cagcctgccg gtaacagcgc gatgatggac
    40261 gcgctggcgg cagcaatggg caaaaaacgt taaacgccct gattgacccc taaacctgcg
    40321 cgataaggtg tccacgccgc atctggcaac gaacgccttg tctgacataa aagtgccgga
    40381 gaatatctcc ggcattttta ttccacagcc aaactcacaa tatattccgg caacatttat
    40441 cacttcatta acaactgaaa ccttaattaa acattagcca gtcctggtaa ttcgctattc
    40501 gaattatatt ttcgctgcga tataaccttg agccacatca acattgagtc agattattat
    40561 tcaaaccaac attcgcacac attttaagta ttgctgatag aaaccattct cattatcatt
    40621 gtgctgttga ttatttaatc tctccttcgt tggcaaatca tctggtctca tgtcgctgtc
    40681 aaacgcccca tgaggtagtt atccagttaa tgagaaacaa gtaggcacct atgcaataca
    40741 ctccagatac tgcgtggaaa atcactggct tttcccatga aatcagcccg gcatatcgcc
    40801 aaaaactgct ttctcttggc atgttacctg gctcctcttt taatgtggtg cgcgtcgctc
    40861 cactcggcga ccccattcat atcgaaaccc gtcgtgtgag cctggtatta cgcaaaaaag
    40921 atctggcctt attagaagtg gaagcggttt cctgttaata cggtgataac aacaatgaaa
    40981 aaattaacca taggcttaat tggtaatcca aattctggca agacaacctt atttaaccag
    41041 ctcactggcg cacgtcagcg tgtaggtaac tgggctggcg ttaccgtcga acgtaaagaa
    41101 gggcaattct ccaccaccga tcatcaggtc acgctggtgg acctgcccgg cacctattct
    41161 ctgaccacca tctcatcgca gacctcgctc gatgagcaaa tcgcctgtca ctacattttg
    41221 agtggcgacg ccgacctgct gattaacgtg gtggatgcgt ctaaccttga gcgtaacctg
    41281 tacctgacgc tacaactgct ggaactcggc attccctgca tcgtggcact gaacatgctc
    41341 gacattgccg agaagcaaaa tattcgtatt gaaattgatg ctctgtcggc gcgtcttggc
    41401 tgcccagtga tcccgctggt ttcaacccgt ggtcgcggta ttgaagcgct caagctggcg
    41461 attgatcgct ataaagctaa cgagaatgtg gaactggtgc attacgcaca gccgctactc
    41521 aacgaagcag attcactggc aaaagtgatg ccttccgaca tcccgctgaa acaacgtcgc
    41581 tggctgggcc tgcaaatgct ggaaggcgat atctacagcc gcgcctacgc cggtgaagcg
    41641 tcgcagcatc tggatgccgc cctcgcccgt ctgcgtaatg agatggacga tccggcgctg
    41701 cacattgccg atgcgcgtta ccagtgcatt gctgccatct gtgatgtggt aagcaacacc
    41761 ctgacggcag aacccagccg tttcaccact gcggtagata aaatcgtgct caaccgtttc
    41821 ctcggtctgc cgattttcct ctttgtgatg tacctgatgt tcctgctggc tatcaacatc
    41881 ggcggggcgt tacagccgct gtttgacgtc ggctccgtgg cgctatttgt gcatggtatt
    41941 caatggattg gctacacgct ccacttcccg gactggctga ctatcttcct cgcccagggc
    42001 ctgggtggcg gcattaacac cgtgctgcca ctggtgccgc agattggcat gatgtacctg
    42061 ttcctctcct tccttgagga ctccgggtat atggcgcgtg cggcgtttgt gatggaccgt
    42121 ctgatgcagg cgctgggctt gccggggaaa tcctttgtgc cgctgatcgt cggtttcggt
    42181 tgtaacgtac cgtcggtaat gggtgcacgt acgcttgatg caccgcgtga acgtctgatg
    42241 accatcatga tggcaccgtt tatgtcctgc ggcgcgcgtc tggctatctt cgcagtattc
    42301 gcggctgcct tcttcgggca gaacggtgcg ctggcggtct tctcgctgta tatgctgggt
    42361 attgtgatgg cggtgctgac tggcctgatg ctcaagtaca ccatcatgcg cggtgaagcg
    42421 acgccgtttg tcatggagtt gccggtctat catgtaccac acgttaaaag cctgattatc
    42481 cagacctggc agcgtctgaa aggcttcgtt ctgcgtgctg gtaaagtgat catcatcgtc
    42541 agcattttcc tgagcgcttt caacagcttc tcgctgagcg ggaaaatcgt cgataacatc
    42601 aacgactcgg cgctggcgtc cgtcagccgg gtgatcaccc cggtcttcaa gccaattggc
    42661 gtgcatgaag ataactggca ggcaacggtt ggcctgttta caggtgccat ggcgaaagaa
    42721 gtggtagtgg gtacgctcaa caccctctac accgcagaaa atattcagga cgaagagttc
    42781 aatccggcag aatttaacct cggtgaagag ctgttcagtg cgatagatga aacctggcag
    42841 agcctgaaag acaccttcag ccttagcgta ctgatgaacc ccattgaagc cagcaaaggc
    42901 gacggcgaaa tgggtaccgg ggcgatgggc gtgatggatc agaaattcgg tagcgcagca
    42961 gcagcttaca gctacctgat tttcgtcctg ctgtatgtac catgtatctc ggtgatggga
    43021 gccatcgccc gtgaatcaag ccgtggctgg atgggcttct ccatcctgtg ggggctgaat
    43081 atcgcttact cgctggcaac attgttctat caggtcgcca gctacagtca gcacccaact
    43141 tacagcctgg tgtgcattct ggcggttatc ctgtttaaca tcgtggttat cggtctgctg
    43201 cgccgcgcgc gtagccgggt ggatatcgaa ctgctggcaa cccgcaagtc ggtaagcagt
    43261 tgctgcgcag ccagcaccac cggtgattgc cattaatggc ttcacttatt caggtgcgcg
    43321 atttgctggc gttacggggc cgtatggaag cggcccagat aagccagaca ttgaacactc
    43381 cacagccaat gattaacgcc atgctgcaac aactggaaag tatgggcaaa gccgtgcgga
    43441 ttcaggaaga acctgacggc tgcctctctg gcagttgtaa aagctgcccg gaaggaaaag
    43501 cctgtctgcg cgagtggtgg gcgctgcgtt aaccttactc catcgcctgt tttggaaagc
    43561 ccggtatgcg tctgcatccg ggcttttttg cgtgcggctt tccataaaaa tgcaactctt
    43621 gcagcacggc gtaagttcct ttgaaagcat ctcgcaggga tgaaaactcg ctaatgcaca
    43681 gatgtggagt ggcgcgtaga gtcgcggcat tcaaacaaca ggtgaaggaa cgccatgagt
    43741 aaaaagcaga gttccacccc acatgatgcg ctgttcaaac tctttttacg ccaaccggac
    43801 acggctcgcg attttcttgc gttccattta ccggccccca ttcacgcgct ctgtgatatg
    43861 aaaaccctca agctggagtc gagcagcttt attgatgacg atctgcgtga aagctattcc
    43921 gatgtgctgt ggtcggtgaa aacggaacaa ggaccaggat acatctattg tctgattgaa
    43981 catcaaagca cctcaaacaa actgatcgca tttcgcatga tgcgttacgc tattgccgca
    44041 atgcaaaatc accttgatgc tggatacaaa acgttgccga tggtggtgct attgttgttt
    44101 taccacggta ttgaaagccc ctatccctat tcgctgtgtt ggctggattg tttcgccgat
    44161 cccaaactgg caaggcagct ttatgcctcc gcatttccgc tgattgatgt caccgtcatg
    44221 cctgatgatg aaatcatgca gcaccgacgt atggcgctgc tggagttaat tcaaaaacat
    44281 attcgtcaac gcgacctgat ggggctggta gagcaaatgg cctgcttatt aagtagtgga
    44341 tacgctaatg acagacaaat caaagggctg tttaattaca tactgcaaac cggcgacgcg
    44401 gtacgtttta acgattttat cgacggcgtt gccgaacgtt caccgaaaca caaggagagt
    44461 ttaatgacaa ttgcggaaag attgcggcag gagggggaac aatccaaagc cctgcatata
    44521 gccaaaataa tgcttgaatc cggagtccct cttacagaca tcatgcgctt taccggggtg
    44581 tcagaagaag agttggctgc ggcgagccag taaatttctg tctcgccatt tcaaaagttg
    44641 cctacaccct ctgcttcaac gccaccagca ggtgacaaaa ctcgaccgga tgcgaaataa
    44701 acggcgcatg agccgctttg gcgaagatat atgattcgct gtgaggccaa agtttatcca
    44761 gcatcggcac cactttgcgc ggcaccagac cgtcgagata gccatacaat cgcaaaaacg
    44821 gcatgggcac gttttgcagc ggctgacgga gatcgaccgt tttcaggatt tccagcccgc
    44881 cattaagcac gtcaacctcc ggcatcggta acgccagaac ggttttcttc aacgcccgcg
    44941 catcctggcg cgccgtttca gtccccatgg tttgtaacgc caggaaccgc tccactgtac
    45001 gctgaaaatc atcactgagt tgctgctgaa atcccgccag cacgtccggt tttatccccg
    45061 gccactcgtc acgagcacta aaacaaggtg acgacgccac ggtgaccagc gcctgaacac
    45121 gctcgggatg ggttaacgca atctggcttg ccaccagccc gcccagactc cagcctaacc
    45181 aaatggcttt atcaggtgcc tgttgcagca cggcttcggc catatcagca agtgacagcg
    45241 caccaaatcc ccggctacgc ccgaagccgg gcaggtcaac aaggtgcagc gtaaaatgcg
    45301 agctaagttc ctcgtcaatg caacgccaca cttcggcatt cagtccccat ccgtgcagca
    45361 gcacaagatg aacattcccc tgacctttgg tctgccacca gatgttattc atccgctatt
    45421 gttctctttt gacttacaag gatgaacata tgctaacagt accgggatta tgctggctat
    45481 gccgaatgcc actggcgtta ggtcattggg ggatttgttc ggtctgctca cgcgctgccc
    45541 gtacagataa aacgttatgt ccacaatgtg gattacccgc cacacactca catcttccct
    45601 gcggtcgctg cctgcaaaaa ccaccgccct ggcaaagact ggtcacggtt gccgactatg
    45661 cgccgccgtt aagtccgctt atccaccagc ttaaattttc ccggcgcagt gaaatcgcca
    45721 gcgccctgtc acgtctgtta ctattggaag tcttacacgc tcgtcgcacc accgggttgc
    45781 aattgccgga tcgcatcatc agcgttccgt tatggcagcg gcgtcactgg cgtcggggat
    45841 ttaatcagag cgatttgctg tgtcagccgt tatcacgctg gttgcactgc caatgggata
    45901 gcgaagccgt cacacgtaca cgggccactg cgacccagca ttttctcagt gcccggctgc
    45961 gcaagcgcaa cctgaaaaat gcctttcgtc ttgaattgcc cgtgcaaggt cgccatatgg
    46021 tgattgtgga tgatgtcgtt accaccggaa gtaccgtcgc agagattgcg cagttgcttt
    46081 tacgcaatgg tgcggcgact gtccaggtct ggtgcctttg tcgaaccttg tagagcctcg
    46141 atgatgggcg tattataacc aactaaaata gtcaactatt aggccattac tatgatccgt
    46201 atttccgatg ctgcacaagc gcactttgcc aaactgctgg caaatcagga agaagggaca
    46261 caaatccgcg tatttgtgat taaccctggc acgcctaacg ctgaatgtgg tgtttcttat
    46321 tgtccgccgg atgccgtgga agccaccgac actgccctga aatttgacct gctgaccgcg
    46381 tatgttgatg agttaagcgc accatacctg gaagatgcag agatcgactt tgttaccgac
    46441 cagttgggtt ctcagttaac gctgaaagcc ccgaacgcca aaatgcgtaa agtggcagac
    46501 gatgcaccgc tgatggagcg cgtggagtat atgctgcagt cgcagatcaa cccacagctt
    46561 gctggtcacg gtggtcgcgt ttcgctgatg gaaatcacag aagacggtta cgccattctg
    46621 caatttggcg gcggctgtaa cggttgttcc atggtcgatg tgacgctgaa agaagggatc
    46681 gagaagcagc tgctgaacga attcccggag ctgaaaggtg tgcgcgatct caccgaacac
    46741 cagcgcggcg aacactccta ctactaagtt atcccctctt ttgtagattg cccgatgcga
    46801 cgctaaagcg tcatttcggg cctacgttga acatgtgcca gacgttggca cagcattgct
    46861 ttggcaaatc cctcccttcg tttccccgcg ataatatgac caacctctca taatttaaat
    46921 ttaccccgct ctggtgattc tcaaacgcca gatgttaccc gtatcattca catgggtacc
    46981 aaacatactc ctgacatctg actacaataa ttagttttag tgggtatcag tcgtggtgcc
    47041 gcaatatctc tgttcccgat tgggataatt agagtttgtc gtcagaaaat tgacgttacc
    47101 cataacaaat gaaaggccag gtaaatcatg ccattagtca ttgttgctat cggtgtaatc
    47161 ttgttgttgc tcctgatgat ccgcttcaaa atgaacggct tcatcgctct cgtcctcgtg
    47221 gcgcttgctg ttggattaat gcaaggaatg ccgctggata aagttattgg ctccatcaaa
    47281 gccggtgtcg gcgggacgct cggtagcctt gccctgatca tgggttttgg cgcaatgctg
    47341 ggcaaaatgc tggcagactg cggtggcgca caacgtatcg ccaccacgct gattgccaaa
    47401 tttggtaaaa aacacatcca gtgggcggtg gtactgaccg gttttaccgt tggttttgcc
    47461 ctgttctatg aagtgggctt tgtgctgatg ctgccgctgg tgtttaccat cgcggcttct
    47521 gcgaatattc cactgttgta tgttggtgta ccaatggcag ctgcactgtc tgtgacccac
    47581 ggcttcctgc caccgcatcc gggtccgact gcgattgcca ccattttcaa tgccgatatg
    47641 ggtaaaaccc tgctgtacgg tactattctg gcaatcccga ccgtgattct cgccggtccg
    47701 gtttacgctc gcgtgttgaa aggtatcgat aagccaattc cggaaggtct ctacagcgcg
    47761 aaaaccttca gcgaagaaga gatgccgagc tttggcgtca gcgtctggac ttctctggtg
    47821 ccggttgtgc tgatggcgat gcgtgcgatt gccgaaatga tcctgccgaa aggtcacgct
    47881 ttcctgccgg tagcggagtt cctcggtgac ccggtaatgg caacgctgat tgccgtgctg
    47941 attgcgatgt tcacctttgg tctgaaccgt ggtcgttcaa tggatcagat taacgacacg
    48001 ctggtttctt ccatcaaaat cattgcgatg atgctgttga tcatcggtgg tggcggtgcg
    48061 ttcaagcagg tgctggtaga cagcggcgtg gacaaataca ttgcttccat gatgcacgaa
    48121 accaacattt ctccgctgct gatggcctgg tcgattgctg ccgtactgcg tatcgcgctg
    48181 ggttccgcaa ccgttgcggc aatcactgcg ggtggtatcg cggcaccgct gattgcaacg
    48241 acgggtgtta gcccagagct gatggttatt gcggtgggtt ccggtagtgt gattttctct
    48301 catgtgaacg atccgggctt ctggctgttc aaagagtact ttaacctgac tatcggcgag
    48361 accatcaaat cctggtcgat gctggaaacc attatctcgg tgtgcggtct ggtaggctgt
    48421 ctgctgctga atatggtgat ttgataacac tgactgccgg atgcatcgca tccggcatca
    48481 accgcactct acttcttctt cgctgcagct ctgcgccgtc tgtccaaatc cttcagcaac
    48541 ttgttcacgc catcatcggc aaacatcgtc tcaagcgttg cggaaagctt gcgtcgccag
    48601 tttttatact ggtaactggt gccaggaata ttcaccggtt cggccatatc cagccagtct
    48661 tccggctgta gtcctaacag agcactgtta ctgtcggcaa tgtagcgctg caaaccacgg
    48721 ttcagcgtcg gcgtcatcga catcaacgat gccttatgcc cggcacgttt tggcagacaa
    48781 ccatatttat gcagtgcatc cagcagccct tgcttcgcca gttcgcgatc ctgatacaga
    48841 ccgcgcagta ccacttcatc cggatacagc cccagggttt tgcccagcgt tagatccccg
    48901 ctctcccagt aaccgcgtag cgtaggcagg tcatgtgtcg ccgcaaccgc catcgactgc
    48961 tccggatacg ctttcggtgc acggaacgtc ttctcgtggt cgttttcgaa atagagcact
    49021 ttgtaggagt acacaccgct gctgcgcagc ttaccgacaa tctctaccgg tacggtaccg
    49081 agatcttcac caatcaccat acagcgatga cgtttacttt cgagtgccag aatcgagagc
    49141 agatcatcca ccggatagtg aacatacgcg ccctgatctg ccgtctcgcc atacggtatc
    49201 caccacaaac gcagcatcga catcacatgg tcaattcgta atgcgccgca gttttgcata
    49261 ttggcacgca acagctcgat aaacggttca taggcacgcg cggtgatgat atgcgggtcc
    49321 attggcggta atccccagtt ctgccccaac gggccgagga tatccggcgg cgcgccaacc
    49381 gacgctttca ggcaatatag ttcacggtca caccaggttt ccgccccacc ttccgctacg
    49441 ccaaccgcca gatcacgata caagccaatc ggcatttcat agccctggct tatctcccag
    49501 caggcggcaa actggctgta agccagccac tgcaaccaga gataaaaatc gacgtcatta
    49561 cgatgttctt cgcagaactg acgcacttct ggtgaatcca cgttctgata catctctggc
    49621 catgcaggcc agccccagcg catttcgtcc tctttcactt gctgggcatg tagcgcatca
    49681 aaggctgcct gccagaacag gctgtcgccc tgctctgcaa caaactggcg aaacgcggtc
    49741 atctgctcat catcacgttg cgcgaaacct ttccacgcca ttcgtaatgc tgtcattttt
    49801 agggcggtaa ccgtggagta atcgacccag tcggcatcgc gcgcctgttg cagcgtctgt
    49861 tgcgtggtcg gcaactgcca ccaggcctga gcctcttcgc taagatggaa atcttcaacg
    49921 gcgttaacgt cgatataaat cacattcagc caacggcgag aagacgggct gtatgggctg
    49981 gcactctccg gatttgccgg atagagcgca tgaatcgggt tcaggccgat gaacgacccg
    50041 ccacgttttg ccacatccac cagcatcgct ttgagatcgc caaaatcccc aataccccag
    50101 tttttttccg atcgcagcgt ataaagctga acgcaggcac cccacagctt ttgtttattc
    50161 agcaacgcct gcggttcgta acagcgtttc ggggcgacaa tcacccggca atgcgcgcgc
    50221 tggtcgtcct gggtgagtgt cagcgtgtga taaccttccg gcagcttcgt cggtagattg
    50281 aacgctttgc cccccgttac atggcctttg tactgtgttc cttcttcggt ggtcagcagc
    50341 cagctatatt cgccgctgcc ctccaccacc atcggcattt ttttgccgct ggtataaacc
    50401 atgacattcg ggactggcgt taccgccact ttcgtggcgg tacgttgatg catcgcgtca
    50461 agcaaacgcc gtttggtttc ggcgctaatc gactgcggtt taccgtgggc attgatgtaa
    50521 ttggggctaa tccccgccgc cagcgcggca ttatccagac gtttgctttc catcgagctt
    50581 ccttagcgtt ttgcctgcca gatacgagcc tgataatcgc gaatagagcg atccgagcta
    50641 aacataccgc agcgggcggt attgaggatc gccgcgcgag tccaggcctc ctggtcgcgg
    50701 tacagcacat ccacctgctt ttgtgcctca acataggctg cgaaatccgc catcaccaga
    50761 tacggatcgc cgccttgttt gccgatactg tgcagcatct ggtcgaaggc atgcttatcg
    50821 ccgtcgctgt atttaccgct ttccagctct ttcaatactg cgtccagcac cttatctttc
    50881 ttccgccatt tcaccgggtc gtagcctttg gccagaatag ccttcacttg ttccacggta
    50941 tgaccaaaaa taaagatatt ttcttcaccg actttctcgg cgatttcaac gttcgcacca
    51001 tccagcgtac cgacagtaag cgcaccattg agcgccagtt tcatattgcc ggtaccggaa
    51061 gcttctttac ctgcagtcga aatttgttcg gagatatccg ccgccgggat cagtttttcc
    51121 gccgccgaaa cgcaataatc cggcaggaac accaccttca acttatcgcc aaccagtgga
    51181 tcgttgttga tcacgtcagc cactttgttg atcgcaaaga taatattctt cgccaggtag
    51241 tagcccggtg ccgctttcgc gccgaagagg aagacgcgcg gtacgcgatc agcctgcggg
    51301 ttttcacgaa tttctttgta caacgccaga atatgcagca gattcaggtg ctggcgtttg
    51361 tactcatgca gacgtttgat ctgaatatcg aaaatcgcct gtggattgat ctcaataccg
    51421 gtacgaactt tcacaaactc cgccagacgg actttattcg cctgcttgat ctcgcgatat
    51481 tgctgacgga atttcgcatc atcagcgaat ttttccagat tgatcagctg atcgagatcg
    51541 ttagcccact ctttttgcag tgatttatcc aacagagccg ccagtgcagg gttgcactgt
    51601 ttgatccagc gacgtggggt aataccgttg gtgacgttat ggaatttgtt cggccatagc
    51661 tggtgatatt ccgggaacag atctttcacc accagatccg agtgcagcgc cgcaacaccg
    51721 ttcaccgcga aaccgccaac cacacacagg ttcgccatat gcacttgttt atcgtgcacc
    51781 accgccagtt tggcccacac tttttcatcg cccggccagg ttttctccac cagcgtttta
    51841 aagcgagtat taatttcgtt aataatctgc atatggcgcg gcagtaagcc tttcaccagt
    51901 ttcacatccc agcgttccag cgcttctggc atcagggtat ggttggtgta agcgaaagtt
    51961 ttgctggtaa tggcccaggc gtcatcccag ctcatctggt gctcatcgat cagcacgcgc
    52021 agcagttctg gaatcgcgat agttgggtgg gtatcgttca gctgaataac ttcgtaatcc
    52081 gccagttcgt gcagtttacg ccccgccaga tgatggcgac gcaaaatatc cgctaccgaa
    52141 caggcacact ggaagtactg ctgcatcaag cgcagctttt taccggcagt atggttgtcg
    52201 tttggataga gaactttggt cagtttttcc gcattgatgc cctgctgttc ggcacgcagg
    52261 aaatcaccgt cgttaaattt agtcagatca aacggatgcg cgtgcgtcgc ctgccacaga
    52321 cgcagcggct gcgccacgcc attacgatag ccgacaacgg ggagatccca cgcttgaccg
    52381 gtaatggtaa actccggctc ccagcgtccg tctttcgtca ctttaccgcc aatccctacc
    52441 tgcacatcca gtgcttcgtt gtggcggaac cacgggtagt tactgcgatg ccagtcatcc
    52501 ggcgcttcaa cctgtttgcc atcgacaaaa gactggcgga acaaaccata ttgatagttc
    52561 agaccgtaac ccgtcgcaga ctgaccgaca gttgccattg agtcgaggaa gcacgccgcc
    52621 agacgtccca gaccaccgtt acccagcgcc gggtcgatct cttcttccag caggtccgtc
    52681 agattgatgt cataagcctt caacgaatcc tgtacatcct gataccagcc gagattcaac
    52741 aggttgttgc ccgtcaggcg accaatcaaa aactccattg agatgtagtt aacatgtcgc
    52801 tgattcgcca ccggcttggc gaatggctga gcacgcagca tttcggccag tgcttcactc
    52861 actgctagcc accactggcg aggagtcatt tcagccgcag aatttaagcc ataacgctgc
    52921 cactgacgtg aaagcgcttc ctgaaattgc ttatcgttaa aaataggttg tgacatagga
    52981 gttccacttt tcttagattt tcaacacaac gttatcgcta gtttgccagg ctcgatgttg
    53041 accttcctca tcctgcgggg gattaggcag ggaggagttg cggggatgag caaggaaatg
    53101 tgatctcaac cacttaaagc tagtgcaaac cacaggatta gcagcaaatc aatgcaatac
    53161 agcgcagaaa atctgtatct aagtgcaaaa aatggccgtt gcgtattttc aaaaagcgga
    53221 aggtaactct ataaattaag taaaggagtg aaacagt
//
LOCUS       DBANUU010000011        52076 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000011 DBANUU010000000
VERSION     DBANUU010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.011"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..524
                     /locus_tag="ACIE68_000641"
     CDS             <1..524
                     /locus_tag="ACIE68_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFX5907009.1"
                     /translation="LCMYGLSKDINKINAVIAGVMYLFLLSLVAIVVASLAPVPAIIV
                     VFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERPVWVISC
                     LMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFGILAAIA
                     ITLVWQRHTRFFH"
     gene            complement(560..1516)
                     /gene="ybhN"
                     /locus_tag="ACIE68_000642"
     CDS             complement(560..1516)
                     /gene="ybhN"
                     /locus_tag="ACIE68_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFX5907010.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1516..2757)
                     /gene="clsB"
                     /locus_tag="ACIE68_000643"
     CDS             complement(1516..2757)
                     /gene="clsB"
                     /locus_tag="ACIE68_000643"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFX5907011.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2754..3515)
                     /gene="ybhP"
                     /locus_tag="ACIE68_000644"
     CDS             complement(2754..3515)
                     /gene="ybhP"
                     /locus_tag="ACIE68_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFX5907012.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3648..4058
                     /gene="ybhQ"
                     /locus_tag="ACIE68_000645"
     CDS             3648..4058
                     /gene="ybhQ"
                     /locus_tag="ACIE68_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFX5907013.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(4020..5126)
                     /gene="ybhR"
                     /locus_tag="ACIE68_000646"
     CDS             complement(4020..5126)
                     /gene="ybhR"
                     /locus_tag="ACIE68_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFX5907014.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(5137..6270)
                     /gene="ybhS"
                     /locus_tag="ACIE68_000647"
     CDS             complement(5137..6270)
                     /gene="ybhS"
                     /locus_tag="ACIE68_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFX5907015.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6263..7999)
                     /gene="ybhF"
                     /locus_tag="ACIE68_000648"
     CDS             complement(6263..7999)
                     /gene="ybhF"
                     /locus_tag="ACIE68_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFX5907016.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVASLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVDGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(7992..8987)
                     /gene="hlyD"
                     /locus_tag="ACIE68_000649"
     CDS             complement(7992..8987)
                     /gene="hlyD"
                     /locus_tag="ACIE68_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFX5907017.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLILA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(8990..9661)
                     /gene="cecR"
                     /locus_tag="ACIE68_000650"
     CDS             complement(8990..9661)
                     /gene="cecR"
                     /locus_tag="ACIE68_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFX5907018.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9890..11254
                     /gene="rhlE"
                     /locus_tag="ACIE68_000651"
     CDS             9890..11254
                     /gene="rhlE"
                     /locus_tag="ACIE68_000651"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFX5907019.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(11486..11968)
                     /gene="ybiA"
                     /locus_tag="ACIE68_000652"
     CDS             complement(11486..11968)
                     /gene="ybiA"
                     /locus_tag="ACIE68_000652"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFX5907020.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            12088..14238
                     /gene="dinG"
                     /locus_tag="ACIE68_000653"
     CDS             12088..14238
                     /gene="dinG"
                     /locus_tag="ACIE68_000653"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFX5907021.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVTKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            14266..15228
                     /gene="ybiB"
                     /locus_tag="ACIE68_000654"
     CDS             14266..15228
                     /gene="ybiB"
                     /locus_tag="ACIE68_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFX5907022.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            15369..16454
                     /gene="hcxB"
                     /locus_tag="ACIE68_000655"
     CDS             15369..16454
                     /gene="hcxB"
                     /locus_tag="ACIE68_000655"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFX5907023.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            16521..16580
                     /gene="ybiE"
                     /locus_tag="ACIE68_000656"
     CDS             16521..16580
                     /gene="ybiE"
                     /locus_tag="ACIE68_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFX5907024.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(16683..16943)
                     /gene="ybiJ"
                     /locus_tag="ACIE68_000657"
     CDS             complement(16683..16943)
                     /gene="ybiJ"
                     /locus_tag="ACIE68_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFX5907025.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(17208..17474)
                     /gene="ybiI"
                     /locus_tag="ACIE68_000658"
     CDS             complement(17208..17474)
                     /gene="ybiI"
                     /locus_tag="ACIE68_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFX5907026.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(17548..18225)
                     /gene="ybiX"
                     /locus_tag="ACIE68_000659"
     CDS             complement(17548..18225)
                     /gene="ybiX"
                     /locus_tag="ACIE68_000659"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFX5907027.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(18267..20549)
                     /gene="fiu"
                     /locus_tag="ACIE68_000660"
     CDS             complement(18267..20549)
                     /gene="fiu"
                     /locus_tag="ACIE68_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFX5907028.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLMNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(20814..21074)
                     /gene="mcbA"
                     /locus_tag="ACIE68_000661"
     CDS             complement(20814..21074)
                     /gene="mcbA"
                     /locus_tag="ACIE68_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFX5907029.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            21350..22276
                     /gene="rlmF"
                     /locus_tag="ACIE68_000662"
     CDS             21350..22276
                     /gene="rlmF"
                     /locus_tag="ACIE68_000662"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFX5907030.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISSNPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(22273..24498)
                     /gene="ybiO"
                     /locus_tag="ACIE68_000663"
     CDS             complement(22273..24498)
                     /gene="ybiO"
                     /locus_tag="ACIE68_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFX5907031.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(24759..25481)
                     /gene="glnQ"
                     /locus_tag="ACIE68_000664"
     CDS             complement(24759..25481)
                     /gene="glnQ"
                     /locus_tag="ACIE68_000664"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFX5907032.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(25478..26137)
                     /gene="glnP"
                     /locus_tag="ACIE68_000665"
     CDS             complement(25478..26137)
                     /gene="glnP"
                     /locus_tag="ACIE68_000665"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFX5907033.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(26276..27022)
                     /gene="glnH"
                     /locus_tag="ACIE68_000666"
     CDS             complement(26276..27022)
                     /gene="glnH"
                     /locus_tag="ACIE68_000666"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFX5907034.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(27426..27929)
                     /gene="dps"
                     /locus_tag="ACIE68_000667"
                     /gene_synonym="pexB"
     CDS             complement(27426..27929)
                     /gene="dps"
                     /locus_tag="ACIE68_000667"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFX5907035.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(28228..29115)
                     /gene="rhtA"
                     /locus_tag="ACIE68_000668"
     CDS             complement(28228..29115)
                     /gene="rhtA"
                     /locus_tag="ACIE68_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFX5907036.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            29350..29415
                     /locus_tag="ACIE68_000669"
     CDS             29350..29415
                     /locus_tag="ACIE68_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5907037.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            29468..29983
                     /gene="ompX"
                     /locus_tag="ACIE68_000670"
     CDS             29468..29983
                     /gene="ompX"
                     /locus_tag="ACIE68_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFX5907038.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(30032..31615)
                     /gene="opgE"
                     /locus_tag="ACIE68_000671"
     CDS             complement(30032..31615)
                     /gene="opgE"
                     /locus_tag="ACIE68_000671"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFX5907039.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(31887..32015)
                     /gene="mntS"
                     /locus_tag="ACIE68_000672"
     CDS             complement(31887..32015)
                     /gene="mntS"
                     /locus_tag="ACIE68_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFX5907040.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            32201..32668
                     /gene="mntR"
                     /locus_tag="ACIE68_000673"
     CDS             32201..32668
                     /gene="mntR"
                     /locus_tag="ACIE68_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFX5907041.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            32665..33783
                     /gene="ybiR"
                     /locus_tag="ACIE68_000674"
     CDS             32665..33783
                     /gene="ybiR"
                     /locus_tag="ACIE68_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFX5907042.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(33842..34762)
                     /gene="ldtB"
                     /locus_tag="ACIE68_000675"
     CDS             complement(33842..34762)
                     /gene="ldtB"
                     /locus_tag="ACIE68_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFX5907043.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            34981..36573
                     /gene="ybiT"
                     /locus_tag="ACIE68_000676"
     CDS             34981..36573
                     /gene="ybiT"
                     /locus_tag="ACIE68_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFX5907044.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            complement(36814..38079)
                     /gene="ybiU"
                     /locus_tag="ACIE68_000677"
     CDS             complement(36814..38079)
                     /gene="ybiU"
                     /locus_tag="ACIE68_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFX5907045.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(38231..39046)
                     /gene="ybiV"
                     /locus_tag="ACIE68_000678"
     CDS             complement(38231..39046)
                     /gene="ybiV"
                     /locus_tag="ACIE68_000678"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFX5907046.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPHFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            complement(39192..41624)
                     /gene="ybiW"
                     /locus_tag="ACIE68_000679"
     CDS             complement(39192..41624)
                     /gene="ybiW"
                     /locus_tag="ACIE68_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFX5907047.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            complement(41630..42529)
                     /gene="ybiY"
                     /locus_tag="ACIE68_000680"
     CDS             complement(41630..42529)
                     /gene="ybiY"
                     /locus_tag="ACIE68_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFX5907048.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            42660..43322
                     /gene="fsa"
                     /locus_tag="ACIE68_000681"
     CDS             42660..43322
                     /gene="fsa"
                     /locus_tag="ACIE68_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFX5907049.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            complement(43398..44147)
                     /gene="moeB"
                     /locus_tag="ACIE68_000682"
     CDS             complement(43398..44147)
                     /gene="moeB"
                     /locus_tag="ACIE68_000682"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFX5907050.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(44147..45382)
                     /gene="moeA"
                     /locus_tag="ACIE68_000683"
     CDS             complement(44147..45382)
                     /gene="moeA"
                     /locus_tag="ACIE68_000683"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFX5907051.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            45586..46551
                     /gene="iaaA"
                     /locus_tag="ACIE68_000684"
     CDS             45586..46551
                     /gene="iaaA"
                     /locus_tag="ACIE68_000684"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFX5907052.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            46538..48409
                     /gene="gsiA"
                     /locus_tag="ACIE68_000685"
     CDS             46538..48409
                     /gene="gsiA"
                     /locus_tag="ACIE68_000685"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFX5907053.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSCKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            48429..49967
                     /gene="gsiB"
                     /locus_tag="ACIE68_000686"
     CDS             48429..49967
                     /gene="gsiB"
                     /locus_tag="ACIE68_000686"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFX5907054.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKVQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            49985..50905
                     /gene="gsiC"
                     /locus_tag="ACIE68_000687"
     CDS             49985..50905
                     /gene="gsiC"
                     /locus_tag="ACIE68_000687"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFX5907055.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            50908..51819
                     /gene="gsiD"
                     /locus_tag="ACIE68_000688"
     CDS             50908..51819
                     /gene="gsiD"
                     /locus_tag="ACIE68_000688"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFX5907056.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
BASE COUNT        12285 a        13272 c        14002 g        12517 t
ORIGIN      
        1 tactttgtat gtatggctta agtaaggata tcaacaagat aaatgccgtc atcgcaggcg
       61 taatgtatct atttttactc tctttggtgg cgattgtcgt tgctagttta gctcctgtac
      121 ctgccattat cgtcgtgttc agcactgcgg gcgcgatgtt tttaatcagt atgctggccg
      181 gtttattatt taatgttgat cctggttctc accgttttat cattatgatg acgttgacag
      241 ggttggccct ggtgatcatc atgaatgccg cattaatgag tgaacgaccc gtttgggtaa
      301 taagttgctt aatgattgtg ttatggtcag gcattatctc gcatggacga aataagctcc
      361 ttgaattggc ggggaaatgc catagtgaag agttgtggag tccggttcgt tgcgctgtta
      421 caggtgcatt aacactctat tactatttta tcggcttctt tgggatactt gccgcgatag
      481 ctataacgct tgtctggcaa aggcatacgc gtttttttca ttagtcgtaa atgcagatac
      541 taaagaactt aactaaactt cacatcgccg cttcattttt cgcccgtagc ttcttcgcct
      601 ggctttccag tagcaggtaa cagatcagcg ccagcagcag cgggataaag taatacagga
      661 cgcgataagc gagtagggcg gcaataattg tgcctttgga tgtatgctcc ccagccagta
      721 gcgcgataaa caccgcttcc agcacaccaa ttcccgccgg aatatgcacg atgacaccag
      781 caatactact aaccagcaac acgcccagta caaagaaata gttcacgctt tgaccaagta
      841 acagccagat aatcgccccc attaccatcc agttaacgct ggaaatcagc atctgagcga
      901 gggcgaattt ccatgaaggc agcaccagct tttgcccttt gatggtcata tggcggtgct
      961 tcgcgaaagc gcaaaaccac aaataaaccg cgataatcat cagtaagcca atgccgagaa
     1021 tacgcagcgt agtttgatcg acataccagt gatccggcaa ctccaccacg cctgcggtaa
     1081 agataatccc tgccagtaaa atatagccca gccagttggt ggtaatactg agcgagaaaa
     1141 tccgcgtaat agtgctgccc ggtaacccta gtcgggagta caaacgatag cgcataccaa
     1201 tgccgccgac ccaggtactg agcgtcaggt tgaaggcgta gcagataaac gacaccagca
     1261 tcacctggcg cttcgccagt ttgtgaccgc agtaaaagcg ggcgagcagg tcatagcagc
     1321 cgtaaatcag atagctgacg accaccagcc cgaccgcact aagcagcgca acgcgattgt
     1381 agtcgcggat gaccttccag acctcttccc agtccacttt tttggcgtag accaccagca
     1441 acacaatcac cgcgataaaa aacagccagg tgaggatctt ctttgctaag cgccaacgcg
     1501 ggtgtgattt actcatcagg gttttacccc cgtgttttca gtttctaccc gatcctgcgt
     1561 ttccattgtc ggttgtgcgg gcggatcaac ctgcgccaga cgtggtgtgt gtgccggaag
     1621 ccagccaacc agcgccggga agtggcgtaa aaagtggaac gccagcacgc ttttggtcag
     1681 gttccaccag gtgcgtttcg gcagcatggt ctcatcgacc tgctgacaat ctgcggcaat
     1741 aatgccgttc agattatcgc gcagcgtctg gttaaaatga cgatcgtgga tgatgacatt
     1801 tgcttcgaga ttcagtgaca aactgagcgg atcgagatta ctggacccta ctgtcgccca
     1861 gtgatcgtcc atcaatgcca ctttgccgtg gagcgggcgg cggcggtact caaatacctg
     1921 aacgccgcct ttaaccagat agttatacag caagcgcgca ccgactctga caatcggcat
     1981 atccggttcg ccctgaatga tcagtttgat ccgcaccccg cgccgtgccg ctttacgcaa
     2041 ggcgtgtaaa aatcgatagc cggggaagaa gtaggcgttg gcgataatga cttctcgccg
     2101 cgcctgagtg agcattttca gatagtgacg ttcaatatca tcgcgatgtt cttcgttatc
     2161 gcgccagacc agcaatacct gcgcttctcc cggctggcgg ttctcttccg ctttgtgatg
     2221 acgtcgccac cagcgtcgtg ccgcgctctg tccaggcagg ttttccagct caaactggag
     2281 aatatcttcg acaatcggcc cttcaaggcg taccgcgtaa tcctgtttag cctctggacc
     2341 gtagctggac atatgctcgg cggagtaatt cagcccgcca ataaaggcga tacgcgcgtc
     2401 gatcaccaca attttgcgat gcatccggcg aaacacattg gtgcgcatac caaaaaggcg
     2461 agggcgggga tcgtagtagc gaaataccac gccagctgcc gtcagttcat tgacaaactc
     2521 atcgctgaga tccggcgaac cgtagccatc cagcaagact tccgctttaa ccccgcgttg
     2581 cgctgctgcc agtagtgccg catgcagctg tttgccgacg tcatcctcaa accagataaa
     2641 cgtttcaaga atgatgcgtt cttgtgcctc gccaatcgcc ttaaacaccg cgggataata
     2701 ttgctcgccg ttttccagca actggatctt attgccttcg cgccagctac atttcataaa
     2761 tgaatctccg cacttaaagg ggcatgatca gaaaggtgtc gccatgtccg cagcggcaac
     2821 gcggttggcg cgctggcgct ggcatttttg acgtagatcc tgtccagtcg tagtagagga
     2881 aattgcaccg gaaacgtgcg cgccgggcgt ccgtgggcgc gggtaaaaat ctcatccagt
     2941 ccggcctgca cttttaacgg atgattagct ttttgccgcc agtcattgaa atcacccgcc
     3001 accaataccg gttcgccgtc cggtagctca ttcacccatt cggcgagcat cgcaagctgc
     3061 gcctgacggt gcgcctcacg caggcccaga tgtacgcaca tcacatgaat cgcttttccg
     3121 gtcatcggtg gcacaatgcg gcagtagagc acgccgcgct tttccgcacc atcgaccgaa
     3181 acatcgcgat tctcataatg ttcaatggga taacgcgaca gtacggcgtt gccgtgatgc
     3241 ccttccgggt atacggcatt gcgaccgtag gcaaaatcgc tccacatcgt gtcggcgaga
     3301 aactcgtagt gcgaggtatc gggccagttt tccacatgca gcggatgaac ttcgtgcgcg
     3361 cccatcactt cctgcaggca aacaatatcg gcgctgacgg tacgcaccgc gtcgcgaagt
     3421 tccggcaaaa tgaagcgtcg gttaaacgcg gtaaaacctt tgtgaatgtt gatggtgagc
     3481 accttgaacg aaaattgttg tgtttgatcg ggcataattt tcctgtcttt gcctcttatc
     3541 tcattgaaat agtgtagtcg gcgtcacaaa aaggtgcggt cttacggaat tttccgtaaa
     3601 gttcggtact ctgagtaagt agagataaat tcttcaggag agaagccatg aagtggcaac
     3661 aacgtgttcg tgtcgcaact ggtctaagtt gctggcagat tatgttgcat ttactggtag
     3721 tggcgctgct ggtggtgggc tggatgagta agactctggt tcacgtcggc gtgggattat
     3781 gcgcactgta ttgtgtcacg gtagtgatga tgttggtgtt tcagcgccac cccgagcaac
     3841 gctggcgtga ggtggcagac gtgctggaag agctgaccac gacctggtat tttggcgcag
     3901 cgctgattgt gctgtggctg ttgtcccgtg tactggaaaa caactttttg ctggcaattg
     3961 cagggctggc aatccttgcc ggcccggcgg tagtgtcttt gctggcgaaa gataagaagt
     4021 tacatcacct tacgtctaaa catcgcgtac gccgctgacc ctgtcgtggc cgttatcacc
     4081 agtagcggcc acaaactatt ccacacaata tccagactcg catccttcaa ataaatctgc
     4141 ttggtaatgt ccgtaaagtg gcgaataggg ttaatccacg tcaggttttg cagccatact
     4201 ggcatgtttt ccaccggaga aacgtaaccg gaaaggagaa tggcgggcat cataaaaaca
     4261 aacacgccga taaacgcctg ctgttgtgtc gagcagagtg atgaaatcaa cagaccgaat
     4321 cccaccagcg ataaaccgta aatcaccatc gtaaagtaga acagcgccag cgatccggcg
     4381 aaggggattt gatacgccca gataccaatc gccagcacaa tggtggcctg gaaggtggcg
     4441 acaattaacg ccggtacggc tttgccgatg aagatctgcc aggtggtgag cggcgaaacc
     4501 agtagctgat cgagcgtacc ttgttcacgt tcgcgggcga cggaaagtga agtgacgatc
     4561 attacgccga tagtggtgat catggcgatc agcgacggca ccacaaacca tttgtagtca
     4621 agattcgggt tataccagtt gcgtaccacc agctcgctgt tgttaggttt cggttttcct
     4681 tccagcagct cctgctgata atttttgacg atctgttgca ggtagttggc ggcaatttgc
     4741 gcactgttgg agttacgccc gtcgaggatc aactgcaacg gcgcggtctg gaaggtatcc
     4801 agtttgcgcg agaagtcagc cgggaaacgc accagtagta acgccttttg tgtgtcgatg
     4861 gttgggcgga tctcctgtgg gcttttcagc agcagcacat gagtaaaggc gctggcgcgg
     4921 gcaaaacgtt gggtcagctc caccgaatgc tcgccgttat cttcatcgta gatggcgatg
     4981 gtggcgttag tcacttccag cgtggcggcg aacgggaaca gaatcacctg aataagcaca
     5041 ggtaaaatca gaatcgcgcg ggtttgcggt tcgcgcagca gcgactgcaa ctctttgcgg
     5101 attaacgtcc ataagcgatg aaacatgctc ttctccctaa tccagccgac gtttggtttt
     5161 cagccacgtc aggccgataa acatcaccgc cgaagcgatc aaaaacagca cgtttaccac
     5221 cagcaccact ggaatattcc cggcgaggaa caggctttgc agggtgctga cgaaataacg
     5281 agcgggaata atgtacgtca ccgcgcggat caccgcgggc atactgtcga tctgaaaaat
     5341 aaagccggaa agcataatcg acggcagaaa agcggcgttc agggcgacct gagcggcatt
     5401 gaactggttg cgggtaatcg tggaaatcag cagccccatc cccagggtac tgagtaaaaa
     5461 caggctggag ataaaaaaca gaatcagcag cgacccgcga tacggcacgc cgagaataaa
     5521 cactgacacc agcatacaca gcaacatcgc cagcatcccg agaaagtaat aagggatcag
     5581 cttacacagc agcagttccg tgcgggtaat ctccgtagag agcagagcct ccatggtgcc
     5641 gcgttcccat tctcgcgcca ccaccagcga ggtgagaatc gcgccgatga ccgtcatgat
     5701 aatggtcacc gcaccgggga taatgaagtg ctggctaatc gccgccgggt taaaccagta
     5761 gcgggtttgt acatcaataa gcggttcaaa agtctgcccg ttgtcctccg ctcgctgcat
     5821 ttgccagatc tgccagatcc cttcgacata cccctgtaca aagttagcgg tattcggctc
     5881 actgccgtcg gtgatcacct gaatcggtgc ggtggcgttg gcgcgctcca tctgttccgc
     5941 aaaatccacc ggaataacca ccagaccgcg aattttcccc gcctgcattt tggcgatcag
     6001 ttcctgacgg ttatcgctga tggtggcgtc gatgtagggc gaaccggtca tggtgtgggt
     6061 gaaatccagc gccgcttcgc tacgctgttc cagtaaaatc ccgacccgca gcttgctgga
     6121 gtcgaggtta atgccgtaac caaaaataaa cagcagtagc agcgggatca ctaccgcaat
     6181 cagccagcta ctcggatcgc gaacgatctg ccgcgtctct ttaacgcaca gcgcccgtac
     6241 gcgacgccag gacaggatcg ggttactcat tgctatgctc cttatcccag tcgtggatca
     6301 actgaataaa ggcttgctcc atggtgggat cgggttgctc gtcgttggcc gactgcgctt
     6361 tcaaatcatc cggcgtgccg ctggcaatta atttcccacg gtacaccagg ccgatgcggt
     6421 cgcaatattc cgcttcatcc ataaagtggg tggtgaccat caccgtgacg cctttctcta
     6481 ccatgctgtt gatgtgcagc caaaattcac ggcgggtgag gggatcaacg ccggacgtcg
     6541 gttcgtcgag aaacagaatg tccggttcat gcatcagcga acaggccagc gccagccgct
     6601 gtttaaaacc taatggcagt tcatcggtgg cgtgggaggc gatacttttc aggccgaatg
     6661 cctcgctcat gcgggagatt ttttcgttct gcgcccgacc gcgtaagcca tacacaccag
     6721 agaaaaagcg taaattctgt tcgaccgtca ggttgccgta gagcgaaaat ttttgcgcca
     6781 tatagccgag atgctggcgt gctttaccgg aactctcttt cagatccatc cccagcacca
     6841 gcgcctgacc agaggtcggc accagcaaac cgcacatcat cttaaaggtg gtcgatttac
     6901 ccgcgccgtt tggccccagc aaaccaaaaa tctccccacg tttaacggca aagttgacgt
     6961 gatcggtggc ggcaaaatca ccaaatttct tggtcagttc tttcgcttcg atcaccgtct
     7021 cgccgggtgt gccgtctacc gtatgcaata ttgcacccag cggcgattct gaggttccgg
     7081 caccgcccag caaatcaata aacgcatctt caaagcgcgg tgtagtttcg ttgatattga
     7141 tttccggcat cccgtcggca tggcgaatat cgtctggtgt ggcctctttt ttgaggatca
     7201 gacgtaccga tttcccctga atcatgccgt cgctgacctg tggcagtttc aaggcgcgtt
     7261 gcaatagttt gcggttgccc tcgtgtggac tggtcatcag aaagctgcgt ccggccatgg
     7321 tttgtgtcag ggcttttggt tccccctgat acagcaactc gccttcgttc atcagcagca
     7381 cgtcacggca ctgctcggct tcgtcgagat acgaggtact ccagaggatt aacatccctt
     7441 cgcctgccag ctcatgcacc atctgccaca gttcgcgccg tgagataggg tcaacgccga
     7501 cgccgggttc atcgagcagc aacactttcg gttcgcccac cagggtacag gccagaccga
     7561 gtttttgttt catcccgccg gagagcttgc ccgccaggcg tccggtaaac ggcccaagag
     7621 acgtaaactc cagcaggcga gcaaaagttt gcttacgtgc ctcgccggtg acgctgcgca
     7681 aatccgcgta cagattgagg ttctccatca ccgtgagatc ttcatacaga ccaaatttct
     7741 gcggcatata gccgagcacg gcgtgcagcg cgccgtcgtt tttgattgga tcaaagccaa
     7801 tcaccgtggc attgccgctg tcgggtttca gtaatcccgc cagcatccgc attagcgtgg
     7861 ttttacctgc accatccggc cccaccagcc ccgtcacata accggcgtga atggtacaat
     7921 cgagcgacgc gacggcgggc ttgtccatgc ccggaaagcg tttttccagg ccgttcagcg
     7981 tgataacggc atcattcatg tcctgcctcg ttaccgaatt gcaccgtcac cggcattccc
     8041 tggcgtaacg catcatcggc gtcggtcacc acaatacgca ggcgatagac gaggtcggta
     8101 cgcagatccg gtgtttcgac ggttttcggg gtaaattcag cagtcggcga aacgaaacca
     8161 atctgcccgt gatacggctt gtccgggcga ccatcggtat aaagcagcac tttgcgcccc
     8221 ggctgggcct ggtcaagatt ccgttcatca acataagcgc gcacccacac cggacgcgtt
     8281 agtgaaacgg taaacaccgt gccaccttca ttgaggaccg tgcctggctc caccgcgcgc
     8341 gttaacagcg tgccatcaga cggggctatc aacgttgagt cctgcaaatt caactccgcc
     8401 tgcgccagtt gcgcctgcgc ctgttcgagg ctggctttcg cctgggcgat gtcctgttca
     8461 cggttaccgg aacggtactg acgcaattta tcctgtgctg atttcagcgt tgcctgcgcc
     8521 tggtcgcgcg aggagcgggc attttccagg tcatttgccg aaatggtgcg gcttttccac
     8581 aacccttgct ggcggttgta gaagttctgc gcatagtcat aggcggcctg cgcctgtttt
     8641 acggctgcgg cggcctgagc gatttcttca tcgcgatacc cggcaagtat caggtcatac
     8701 tgcgcctgcg ccaccgaaac acccgctttc gcctgcatca gggcaatctc atacggcttg
     8761 tgatccagtt cgcccagcac ctggcccgct ttgatagcat caccttcgtc caccgccagc
     8821 gattcaacgc gccccccaac acggaaacta agattcaccg tacgaatatc cacgttgcca
     8881 tacagcgtca ggccgttatc ctggcggctt tgataccacc agtagcctcc ggcaaccacg
     8941 gcggcaagta ccactaccgc cagtccgatc acgacaggtt ttttcatcac tacaaactcc
     9001 tttgcgataa tccttgcaaa atcaggtcga tatgacaggt caccgtctgg ttgatcagtt
     9061 cggtcttttc ttcatcgaac gcggtccagc cggtacgtaa cagaattgtt tctttgccaa
     9121 gacggaacgc cagaatctca ccaatcaacg catgggtatg aaggatcatg cgggtgtcat
     9181 tggcgtcgct gccggtccag gcggcaatca ggcgtgtcag gtggctgtgt agcggactaa
     9241 tcacctgttc gtgcaccagg tggtaggctg ccgtgggaga gagctgctca cgggagataa
     9301 acttgctgag gttgacggtg tcatcctggg tgagcagctt aatcatgttc ctgcaggcgc
     9361 gaaggatcag ttcacggatg gcagcccgat caggctgtgg ttgggcgaac aagcgttcgg
     9421 cttcctcggc atgcggacgg aactgctcgc caataaaatc ggcaatccac tgggcgcagg
     9481 cgaggtacaa atcttctttc gaaccgaagt agtaggtgat ggcagcgata ttctgcccgg
     9541 cctgggcggc tatctcgcgg gtggtggcgt tcattccata ttcaccaaac tgcgccagtg
     9601 cggcagcaat cagctgtttt ttcgcctgtt cacccttgat tgtcatggca ggattattca
     9661 tcgcacagtc cattcttaat caaatgattg attaagatta tgactccata tgggagttgt
     9721 ccagtatggc taagaatttt agctacgcca gtcacaggga taatttatgc gctgcgtcac
     9781 aaaaactgct acactccgct ccctcatgac attgtggttt ttgtcatttt ccttttcagt
     9841 atctccctga aaactacacc ggtaacggtc ggggcggttc ggagtagtta tgtctttcga
     9901 ttctttgggt ttaagccctg atatcctgcg cgccgttgcc gagcagggtt accgtgaacc
     9961 cacccctatt cagcagcagg cgatccctgc ggtgctggaa ggccgcgacc tgatggctag
    10021 cgcccagacc ggtaccggta aaacagcggg ctttacgctg ccgctgttgc aacacctgat
    10081 cactcgccag ccgcacgcca aagggcgtcg tccggtacgt gcgctcattc ttaccccgac
    10141 ccgtgaactg gctgcgcaga ttggcgaaaa cgtccgtgat tacagcaaat atttgaacat
    10201 tcgttcgctg gtggtgtttg gcggtgtcag cattaacccg caaatgatga agctgcgcgg
    10261 cggcgttgat gttctggtgg caacaccggg acgtttgctg gacctggaac atcaaaatgc
    10321 agtgaagctg gatcaggttg aaatcctcgt cctcgatgaa gctgaccgca tgctcgacat
    10381 gggctttatc cacgatatcc gtcgcgtgtt aacaaaactg ccagcgaaac gccagaacct
    10441 gctgttctct gcgaccttct ctgacgatat taaagccctg gcggaaaaac tgttgcacaa
    10501 cccgctggaa atcgaagtgg cacgccgcaa taccgcgtct gatcaggtga cccagcacgt
    10561 tcactttgtc gataagaaac gcaaacgcga attgctgtcg cacatgattg gtaaagggaa
    10621 ctggcagcag gtgctggtgt ttacccgtac caaacacggc gctaaccatc tggctgagca
    10681 gctcaataaa gatggcatcc gtagtgcggc gatccacggg aataaatcgc aaggtgcgcg
    10741 tacccgtgcg ctggctgatt ttaaatcggg tgacattcgc gtactggtgg cgaccgatat
    10801 cgctgcgcgc ggtctggata tcgaagagtt gccgcacgtc gtcaactatg aactgccaaa
    10861 cgtaccggaa gattatgtcc accgtatcgg gcgtaccggt cgtgcggctg ctaccggtga
    10921 agcgttgtcg ctggtgtgtg ttgatgaaca caaactgctg cgtgatatcg aaaaactgct
    10981 gaaaaaagag atcccgcgca ttgcgattcc gggctatgag ccggacccgt caatcaaagc
    11041 cgaaccgatc cagaacggtc gccagcaacg tggcggcggc ggtcgtgggc aaggtggtgg
    11101 tcgcggtcaa cagcaaccac gccgtgggga aggtggcgca aaatctgcaa gcgcgaaacc
    11161 tgcagaaaaa ccgtctcgcc gcctcggcga tgccaaaccg gcaggcgaac aacaacgtcg
    11221 ccgccgtccg cgtaaacctg ccgctgcgca gtaatctttt atgccgggct atgcccggca
    11281 tcaggctgat gaacaaacgc aaaactgtct gatgcgctac gcttatcagg cctacgtgaa
    11341 ctctgcaata tattgaattt gcatgctttt gtaggccgga taaggcgttt acgccgcatc
    11401 cggcatttca caacaagcac ttgtcagcaa tttgagaaca cgggaaaaaa tttatctgtt
    11461 ttaccgccca taagccaccg agacgttact tctctatagc caattgttcg cgcaactcca
    11521 ttaaaaggta gcccagtcga ttcttgccct taccattacc accgtctccc cagtaagcat
    11581 cgttttccgt atgctcaacc agttttgcgg gcgcggttgc cagcaagagc gcacgcagtt
    11641 ctgcatgctg ttcgaattta gcgcgaagtg cttttcgcat cacctgttct ttgaccgact
    11701 cccagttttt acgcagaggc ttagaacgat cgcgccccat gcgtgcggcg accatagggg
    11761 aagaaacccg acggatctct tcgcggtatt tttcatcaag gaatttttgt gcctgaaaat
    11821 agtgttctga ggtgggccag gttttcccgt caaccttgat gggccaggcg gcaaagttag
    11881 aaaaatcacc gtagtcatcg ctggtgctgt agaaatttat gatggtgtct tgcatgacgt
    11941 gttggatcct ttgtgctcga acgggcatta aaccgcatta tgttggtggt tattgcgagc
    12001 cgctttccag aaacagaaaa accattaccc ctgaaaaccg aaaaatgcca caatattggc
    12061 tgtttataca gtatttcagg ttttctcatg gcattaaccg ccgcgcttaa agcgcaaatt
    12121 gccgcctggt ataaggcgct tcaggaacag atccccgact ttattccccg tgcgccgcag
    12181 cggcagatga ttgcggacgt caccaaaacg ctggccggag aagaagggcg gcatctggcg
    12241 attgaagccc ccaccggcgt tgggaaaacg ctctcctatt tgatccccgg catcgccatt
    12301 gcccgcgaag agcaaaaaac gctggtggtg agtaccgcca acgtggcgtt gcaggatcag
    12361 atttacagca aagatttacc gctgctgaaa aagatcattc ccgatcttaa attcactgcc
    12421 gcttttggtc gtgggcgcta cgtttgcccg cgtaatctga cggcgcttgc cagcactgag
    12481 cccacgcaac aggatctgct ggcgtttctt gacgacgaac tgacgccgaa caatcaggaa
    12541 gagcaaaaac gttgtgcgaa gctgaagggc gatctcgaca cttataaatg ggatggtctg
    12601 cgcgatcata ctgatatcgc tatagatgac gatctctggc gtcgtttaag taccgacaaa
    12661 gccagctgcc tcaaccgcaa ttgttactac tatcgtgaat gcccgttttt tgtcgctcgt
    12721 cgggagattc aggaagcgga agtggtggtg gcaaaccatg cgctggtgat ggcggcgatg
    12781 gaaagcgaag cggtattgcc tgacccgaaa aatttactgc tggtgctgga cgaaggtcat
    12841 cacctgccag atgtggcccg ggatgcgctg gagatgagcg ctgaaatcac cgcgccctgg
    12901 tatcgactac agctggactt gttcacgaaa ctggtcgcta cctgcatgga gcagtttcgc
    12961 ccgaagacca tcccaccgct ggcgatccct gaacgtttga atgcgcattg tgaagagttg
    13021 tatgagctta tcgcctcgtt aaacaacatt ctcaatctct acatgcctgc cgggcaggag
    13081 gcagagcacc gttttgcgat gggcgaactg ccagatgaag tgctggagat ctgccagcgg
    13141 ctggcaaaac tcaccgagat gctgcgtggc ctggcggagt tatttcttaa cgatttaagt
    13201 gagaaaaccg gcagccatga cattgtacgt ctgcatcggt tgattttgca gatgaaccgc
    13261 gcgttgggga tgttcgaggc gcaaagcaaa ctctggcggc tggcttcgct ggcgcaatct
    13321 tccggtgcac cggtgaccaa atgggcgacg cgggaagagc gcgaagggca gctacacctc
    13381 tggtttcact gcgtgggaat acgtgtcagc gatcagctgg aaaggctgct gtggcgcagt
    13441 attccgcaca ttattgtcac ctccgcaacc ttgcgttcgc tgaacagttt ttcgcgtttg
    13501 caggagatga gtggtctgaa agagaaagcg ggcgaccgtt ttgtggcgct ggattccccc
    13561 tttaaccact gcgaacaggg caaaattgtt attccccgga tgcgcgttga gccttccatc
    13621 gacaacgaag agcagcatat tgccgaaatg gcggcctttt tccgtaagca ggtggagagc
    13681 aaaaaacatc tcggtatgtt ggtactgttt gccagcggac gggcgatgca gcgctttctc
    13741 gactatgtga cggatttacg tctgatgttg ctggttcagg gcgatcagcc gcgttaccgt
    13801 ttagttgaac tgcaccgcaa acgcgtcgcc aacggtgagc gcagcgtgct ggtgggctta
    13861 cagtcatttg ccgaagggct tgatttgaaa ggtgatctgc tcagccaggt gcatatccac
    13921 aaaatcgctt ttccgcccat cgacagcccg gtggtgatca ccgaagggga atggctgaaa
    13981 agcctcaacc gctatccgtt tgaggtgcaa agcctgccga gcgcctcgtt taacctgatt
    14041 cagcaggttg ggcgactgat tcgaagccac ggttgctggg gcgaagtggt tatctacgac
    14101 aaacgcttgc tgaccaaaaa ttatggcaag cgactactgg atgcattacc ggtatttccg
    14161 atagagcaac cggaagtccc tgaaggtata gttaaaaaga aagaaaaaac gaaatcccca
    14221 cgccgtcggc ggcgttaatg atgtgagtca ggtaaggagt cgtaaatgga ctatcgcaaa
    14281 atcattaaag agatcgggcg cgggaaaaac cacgcgcgcg atttagaccg ggatactgcg
    14341 cgcggtctgt atgctcatat gctcaatggt gaagtccctg accttgagtt gggcggcgta
    14401 ttgattgcgc tgcgtatcaa aggagaaggg gaagcagaga tgctcggctt ttacgaagcc
    14461 atgcaaaatc acaccatcaa gctgacgccg ccagcaggca agccgatgcc gattgtcatc
    14521 cccagttaca acggcgcgcg taaacaggcc aacctgacgc cgttgttggc gattctcctg
    14581 cacaaactcg gttttcctgt ggtggttcac ggggttagcg aagatccaac ccgcgtgctg
    14641 actgaaacca tttttgaatt gatgggtatt acgccaacgt tgcacggcgg gcaggcgcag
    14701 gcgaagctcg acgaacatca accggtgttt atgccagtcg gcgcgttttg cccgccgctg
    14761 gaaaaacaac tggcgatgcg ctggcggatg ggcgtgcgta acagtgcaca taccctggcg
    14821 aaactggcga cgccatttgc cgaaggtgag gcgctgcgtc tttccagcgt ttcgcatccg
    14881 gaatacattg gacgcgtcgc gaagttcttt agcgatatcg gcgggcgggc gctactaatg
    14941 catggtactg aaggtgaagt gtatgctaat ccgcagcgct gcccgcagat caatctcatt
    15001 gaccgtgaag ggatgcgggt gctgtatgaa aaacaggaca ctgctggtag cgagttactg
    15061 ccacaagcaa aagatccgga aaccacggcg cagtggattg agcgttgcct tgctggcagc
    15121 gaaccgattc ccgaatcgct gaaaatccag atggcttgct gcctggtggc tacgggtgaa
    15181 gcggcaacta tcagcgacgg cctggcgcgc gttaatcagg cattttaatt cttttctcag
    15241 ccggatgagc catgtcatcc ggcttttacc ccaccgcaat atgaaattcc tgcatcttta
    15301 ttgaccttcc cacgcccggc gtgcagcata aaaatacaac aaacacataa cataaacagg
    15361 agttaaccat ggaaagtggt catcgctttg atgctcagac gctgcacagt tttattcagg
    15421 ctgtatttcg tcagatgggt agcgaggaac aagaagcgaa attagttgcc gatcatttaa
    15481 tcgcggcaaa cctggcaggg catgattcac atggtattgg catgatccca agctatgtac
    15541 gctcctggag tcaggggcac ctgcaaatta accatcatgc caaaaccgtt aaagaggcgg
    15601 gggcggcggt cacgctcgat ggcgatcgcg catttggtca ggtcgcggca catgaagcga
    15661 tggcgctggg gattgagaaa gcgcatcagc acggtattgc cgccgtggcg ctacataact
    15721 cgcatcatat cggccgtatc ggttactggg cggagcagtg tgcagcggcg gggtttgtct
    15781 ctatccactt tgttagcgtg gtcggtattc caatggtcgc gccgttccac ggtcgcgaca
    15841 gccgctttgg caccaatccg ttctgtgtgg ttttccctcg taaagataat ttcccgctgt
    15901 tgcttgatta cgccaccagc gccattgcat ttggcaaaac ccgcgtcgcc tggcataaag
    15961 gcgtccccgt gccgccaggt tgcctgattg acgttaacgg cgtgccgacg accaatccgg
    16021 cggtaatgca ggagtcgccg ttgggttcgc tgttgacctt tgccgaacat aaaggctacg
    16081 cccttgcagc gatgtgtgaa attcttggcg gggcgctttc cggcggtaaa acgacgcatc
    16141 aggaaacgtt acaaaccagt cccgatgcca ttcttaactg catgaccact atcatcatca
    16201 acccggaact cttcggcgcg ccggattgta acgcgcagac cgaagccttt gccgagtggg
    16261 tgaaagcctc gccgcatgat gatgataagc cgattttgct accgggcgag tgggaagtga
    16321 acacgcgtcg cgaacggcag aagcagggga ttccactgga tgcgggaagc tggcaggcca
    16381 tttgtgatgc agcgcggcag attggtatgc cggaagagac gttgcaggct ttctgtcagc
    16441 agttagccag ctaaaaaaaa gcccgtccag tggcggacgg gcaaacaagg gtaacatagg
    16501 atcaatgagg gttagagcat atgcgtctgt cggcaaacag acagggaaat acttgtgctg
    16561 gacgtagcgt aaacgcctga tccgtccaac ggttcggatt ttgtaggcct gataagacgc
    16621 ggtagcgtcg catcaggcat ctgttgccgg atgcggcgta aacgccttat ccgtcctacg
    16681 aattacttat aaattaccgc agtaccgctt aatttattgt tgttggtggc ggaagtgatg
    16741 ctgtatccac tggcaccggc tgccgcggct ttctcagcca gtttcgcttc cagggcatcg
    16801 agggtggatg cgccatcggc agaaaccacg ccgattttat tcatgttctg tgcctgggat
    16861 gccgttaccg gttccgccgc gaacacgcca aatgacagag ttgaaagagc catagcagca
    16921 acaacagtat tgatagtttt cataattaat ctctcgcagg tgatttttgt ataagtgacg
    16981 ttgtttcgtc gatgtgatga gtatcacgtt tttttacgag agataaaatc gaagagaatt
    17041 gaccgccttg ttcaaataaa ttgattgata tctaacctac tgataaacca tttatttgtt
    17101 gtacttatga acttgtttta taccgcgtgg cagtcacagc agcgttaaag gcacggaatg
    17161 ccacgcaaaa tacaaattat actcgctatg catctcgcca gttgtagtta acgtaactgg
    17221 ctgtctttcg aacctctgcg attatatcct gtataagctg gtttttgtaa atctttctcc
    17281 tgctgacaat gaatacataa gcgcacgcca ggaatggctt cccgacgggc ctgcgggatg
    17341 ggggcaccgc actcttcaca ttcatccagg ctttcgccgc gcggaatttc accccgagcg
    17401 cgggcaatcg catcttcaat tgtactgttg atctgttcgt tgacggcgtc atcgttagcc
    17461 caaccggatg ccatatcgac ctccccatat caatacttgt acagttaagt gtagctaatc
    17521 cagggacgaa ctcgggcagt tcaagcatca gatctccgac cattcccgca gcagattatg
    17581 ataaagatta agcagcgaca ggatctcttc actttcgccg tagcggcttt tcagcgactg
    17641 aatattgttg tccagttcaa acagcatggc gcgcttttta tcatcgcgga tcatcgactg
    17701 gatccacata aatgatgcca ctcgtacgcc gcgggttacg ggtgtcacgc aatgcaggct
    17761 gctggaggga tacaacacga gatcgcctgc cgggagtttt acccgatgtt gtccgaaggt
    17821 gtcattaacg accagttcgc cgccgtcgta gctttgtgga tcgcttaaaa acagcgtggc
    17881 agaaaggtca gtacgcatcc agccgttttg tggatggctg cgtactgcgc catccacatg
    17941 gaaaccatag gtttcattgt tctgatagcg attaaacagc ggcgtggaaa gggtacgcgg
    18001 caaggccgcg gcaaagaata aagcatgttg gttaaccgcg ttcagcacct cattttgcag
    18061 ggcggcgtat aacgtgctgc gggtgtcgac ctgttgattg ttcttaactt gcgcaccttg
    18121 tgcgccggtg gtgacgcgtc catccaccca ttcggcttgt tccagttgtt cgcgaaaacg
    18181 agcgacgtcc tgtggcgata acacgccggg aatgtggtac atcatacaat ttctccaaaa
    18241 agtggggcct gcgccccaca tctgaatcag aaatgcatat tggctgtgag caagaaggtt
    18301 cttggctcgc ccgggtgata acggtagccg ctcttgttga ttgaggcgac gtaatcggta
    18361 tcaaacaggt tgtaaacgtt tagctggaag tcgagattgc gattaactcg ataccccagt
    18421 ttggcatcgg cgacccagta accttcggta aacgctggcg ttcccaccgc gccgtctgaa
    18481 cctttatgca tactgccgat atagcgtgcg cccgcgccaa cagagatatc gtcggttgcc
    18541 tgatattggc tccataaggt gaaggcgtgc tccggggtat acggcagcga tgaggaacca
    18601 tcctgggcaa catctttgcc gtttttgatg gttgcttttt gctgggtata gccgccaatc
    18661 acctgccacg cgggagtgat attcccggcc acggatatct catagccttc gacgcgtttc
    18721 ttaccgtatt gcgagtaagt tccgtcatca ttttgctcaa cttcattttc gatatcagta
    18781 cggaacagcg cggcggtgag caacagacgt ttatccagaa cctgccattt ggtgccaatc
    18841 tcgctggtgt tggctttttg cggtttaaaa tcggtgcggt tggcactgtt accgctgcca
    18901 gactgcgcaa gggcgaagtt gttgccgccc ggaggctgct gggaaacggc atagttaata
    18961 tagacattgc cgttttccgt caggtgatac agcgccccgg ctttccagtt catcagattg
    19021 cccgacttgg cggtgtcgac ggtggtgacc ggagaacctt ttgccacacc agttgggcag
    19081 gtgatggcac cgcgtccgct gccgccgcag gcggtggcac tgtcatattc agtatgataa
    19141 ttatccagac ggatcccgcc gttcagctca aaatcacggg tgatttgcag cgtatcaaag
    19201 gcgtaaattg cgaaggtatc cgtctgacca ttggcgtttg cgccgttgcg cgtcaggccg
    19261 ccgggatgaa tgctgctgtc aggatgataa atatttaccg cgggtaacgt caccggatta
    19321 acgccgtagt tagtctgcgt ttcacgggta aattccacgc cggtactgac atcatgaccg
    19381 atagaaccgg tatagaacgt cgaggtcagg ttggtctggt tggtgagaat tttattactc
    19441 acatctttgg tattcgccgt gcgtgaccag gtccagctat tcacatcgct ggtgggctga
    19501 gtaatattcg acgccccgcc cataatcgcc gtcatcaggt aatcctgctt tacgcgcgac
    19561 caacgggtag tattgcgaat ggtggtgtta tcgttgatgt cgtgctcaaa acgcatggtg
    19621 gcggtgtcgg tggtcgaatc gtcgtaatcg gaatccgtgc cgtaaaagtt atgagtatca
    19681 acttttccgg aatgattcag agctgctgtt cccgcagatg gggcagaata acccggcaaa
    19741 ccgatggtcg gaatgccgcc gtctggcgtg ttgtgctggg tgacatgcag ataattaaga
    19801 tacaaacgat tcgctgtacc aaggccaaaa gcgatagaag gggcgacgcc gtaacgctca
    19861 tttttgactt tgtcgcgtcc ggcatcgtgc gttttttcgc ccatcacatt caggcgcacc
    19921 gcagtggtat caccaatgac ctgattgacg tccagcgtgc cgcggcggaa ccaggcgctg
    19981 ccaatactgg cggaggcgtc aatgccggaa tcattgcgcg gctgcttgct gatcatattg
    20041 atcgagcccg tcggcgcgct gcgcccgtag tcggtgccgg aaggcccttt aatcacttcg
    20101 acctgctcag tattgaaggt gtcgcgcgag acgctgccga tatcgcgaat gccatcaata
    20161 taaatactgt tagaggtatc agcaccgcgc atataaatgg cgtcgccagt ggtggagtta
    20221 ccgttctcac ccgcaaaaaa cgcacccacg ccggggacgt ttttgagcgc gtcggtaagg
    20281 ttggttgcgc cctgatcttt aatcacttgt tcagaaatca ccgtcatcgt gcgggtagta
    20341 tccgctaccg gacgcgagaa tttcggatcg gcagattgtt gtggcgcata aagcgaaggc
    20401 gttgatgctt cgacaaccag cgtgtcatcc gcgttagttt gcccttcggc ggcgagtgcc
    20461 tgagccacag gcgtgatgcc aatacaaaga ccggcaaaga acgtgagcga atgaaattgt
    20521 ctggcaggga aattgcgatt gttttccatt ttgcaggtga ctttttttta tatgttgaaa
    20581 ttttcactgc cacgagaaag cctctctgcg gatgcgatgc cactgttaat ctcgtgatag
    20641 tgttcgtgca agtgataata attttgataa gcattatcac ttggcgcgat tttctatcac
    20701 ccattgcgcg aaaaagaaat acatttattt acattgcgtt catatttgat gttttttgat
    20761 tacaaaacag gcgaaaactg catccagagg cgaatgcagt tacgttggtg gggttatttg
    20821 tagatggttg cagtaccgaa catctggtca tttccgcctg cggaattaat gacataacct
    20881 ttcgcgcctt gctcgcgcgc tttttctgcc agtttatcct ccagatcgct taggtttgat
    20941 gcgccagttg ccgaaacggt accggcgggc cgtagctgac cgctgtccag gttactcatc
    21001 ggttgcgcgg cataagccgt gagagagatt ccgctcagga cggtggcaat cagtagtgtg
    21061 aggcactttt tcataatgac atcctcataa atacaacggg tagtgcctga taagtgtagg
    21121 tcgcctgggc gatgaaaatg acgcaaaaaa ttgacagaga tgtgcttatt ttttgaacga
    21181 tatattttta caaaataaga caaatcaatg acatgcataa tgctgatttt tatgatgaat
    21241 tgccataaac catagccatg ttaaggtttt ggggaatgat aaacgcagcc agatatttac
    21301 cctgtcgcgt tatgcgggta tcttacgccg cagcttaaag gagaatgcta tgtccgccca
    21361 gaaaccgggg ttgcatccgc gcaaccgtca tcacagccgc tacgatctcg ccacgctttg
    21421 tcaggtcaat cctgaactca ggcaattcct cacgcttaca cccgccgggg agcaaagcgt
    21481 agactttgcc aatccgctgg cggtgaaggc gctcaataag gcgttgctgg cccattttta
    21541 cgccgtagcg aactgggata tccccgacgg ttttctctgc ccaccagtac cgggccgggc
    21601 ggattatatt catcaccttg ccgatttact ggcagaagca agcggaacaa ttccagcgaa
    21661 tgccagcatt ctggatatcg gcgttggtgc gaactgtatt tatccgctga ttggcgtaca
    21721 tgaatatggc tggcgtttta ccggtagtga aaccagcagc caggcgttaa gcagtgcgca
    21781 ggcgattatc agttctaatc cggggcttaa ccgcgccatt cgtctgcgtc ggcaaaaaga
    21841 gagtggggcg atttttaacg gcatcatcca taaaaacgag caatacgacg cgaccttgtg
    21901 taacccgcca ttccacgatt ccgctgctgc ggcacgggca ggtagtgagc gtaaacgccg
    21961 taacctgggg ctgaacaaag acgatgcact gaactttggc ggccagcaac aggagttgtg
    22021 gtgtgaaggc ggtgaagtca cctttatcaa aaagatgatt gaagagagca aaggcttcgc
    22081 gaagcaggtg atgtggttta catcactggt atctcgtggt gaaaacttac cgccgttgta
    22141 tcgtgccctg acggacgtgg gcgcggtgaa ggtggttaaa aaagagatgg cccaggggca
    22201 aaagcagagt cgctttattg cctggacctt tatgaacgac gagcagcgcc gccgttttgt
    22261 caatcgccag cgttaaagcg ttggttcccc cggcggtaac ggttcagccg gggtcgcgcc
    22321 cggagcaggc agcacctgat aagtctgcac tggcgcgcga acgcccgcca ggtcgaaatg
    22381 ttttttcacc tggctgtcga gggcaaagcg taccgtccac tgtttgagtg gcagcgtggt
    22441 gaacgaaaca cgcagtgtaa acgcggtatt gcttaagccg acaatcccgg caaaattcgg
    22501 ttcaccaata atcagcccgc gaatttcttc gttttccatt aattccgcta ccgcatcttt
    22561 cagtgcctga ttggctttat cagcatcttc atggcgatca acatcataat ttgccaccac
    22621 cgaaccaatg ccgcggacaa agttagcaaa ggtggttatc gaagaccacg gaatgatgtg
    22681 atacgccccg gtatcctggc gtacgcccac ggagcgaatc gacatccgtt ccactgtgcc
    22741 ggtcaacggc ccgatagtca ccaaatctcc agtgttcatg ccgttttcaa actgaataaa
    22801 taccccggtg ataatatctt tcaccagcgt ttgcgaacca aacgagatag ccagacctaa
    22861 tgccccggca cctgccagca atggcgcgat attgacgccg atttccgaca acacaatcat
    22921 gatggtgatg gtactgataa tcaccgccag cgcgttacga aacagcgtaa gcagggtacg
    22981 cgtgcgggcg ctgggtagcg ggcggccatg aatatccgaa gccagccggt tttcgatcaa
    23041 actggcgagc accgtccagc caaccgccga gaagaaaaga atgagtgcga tacggatcag
    23101 gatatctacg gttttctgcc ccgcgccgtt ttgcagccag ttccagaaat cgaacaatcc
    23161 ccatgcgctc aacagcaaca ttaccgccac gcagactgtc agaatacgcg ccgttttcag
    23221 cgccgccgac agccagccat tcaaccgttt ttgcagctcc ggatagttac gctgagtatg
    23281 tggcgagaga gtgatggttt tcgccagcca gcgggaaaac ataccggaaa caaacgctgc
    23341 gataccaata atcgccaggc tgcgcaccgt tgcacccatc ataaatttca ggctgttgcc
    23401 cggatcgaac aacgaaaaga aaaacagcac gataaaatag gcgcttgcca gccagtgcca
    23461 caccagcgca aaggcgcgga taaacaggct gaaaaaggcc agcgaatgct ccgcgaagtt
    23521 gagcaaatgc tgggtaatct cttttttatt acgaaagatc aggtacaacg cccagacggt
    23581 catgcacagc ataatgatga cgttcgccag cgcacctatc tgtacattca cctgattaga
    23641 gataatcggc acggccacaa tcaggccata acctatcaaa ctgcttaacc agctcaggcg
    23701 acgactccag taacgggcgc tctcgtcctg aatcgtgaac gggcgcagct ccgccacgtt
    23761 tgggcaaaaa atcaggcgta gtacggcttt gaaaaattca atgagggcaa aggcgttgag
    23821 aaacaaactt tgttggaaag cgatggtgcg actgcctgca ttcaggttgt cgcttaatac
    23881 ctggccgaca aataatgtca gggccagtaa cagcaggtcg ataataaacg ccccgataat
    23941 catcgccgga agctgcaacc agttgctgcg ctcacgattt ttttgccgcg cccactgacc
    24001 cattttgcga tacagcggca gtgcgcacag gcgaatcagc cagtaaaaac caaacactaa
    24061 taccgctaac attgaaaaat gggtcagcgc attgctgaag gtttgtggat taaacggctt
    24121 atgcggggag ccggtgatat tgcgataaag ttgcccgaag cgggcggaaa gggcttcacc
    24181 ataatggcgg ctgacttcgg tgaccttttg cagcacggtt tgctcttcga ccagcgtcgg
    24241 cggcactatt tttggtaccg gttcagcagg gggcgtagcg gcaacggtgc gcaactggtc
    24301 gatcaactct ttacgcgagg tgtcattatc cagcacatcc gccagtgcgc cataggccgc
    24361 ttttttttgt tcgatatccg gttccggggc cggttcagtc gttgagtccg ttgtcgttgt
    24421 ggttgtaacg ccgggtatgg ataccgcgtg ggcaggtgcg cccagcaggc agaagaggat
    24481 gaacaggatc caccgcatga ctcctccagt gagaaaatag agcaaaaaga taagtataga
    24541 tgctggagga ggggcgattt taaatgagag gaatctggtg tgcctccctt tcgggtgaaa
    24601 gggaggaagg gatattaagc aggttgctga caacgtgcgg gcttttttgc cggatgcggc
    24661 gtgaacgcct tatccgtcct acaagttcat gcaattttaa taagttgcca gatatcctgt
    24721 aggcctgata agcgtagcgc atcaggcaat gtgtcttatt aagagacgtg ctgcaaaaat
    24781 tcctgcaagc gctggctcgg cgggttcttg atcaacacct gcggattgcc atcttccgca
    24841 atccggcctt tgtcgataaa gatcagccgc gaagctactt tctcggcaaa accgatttcg
    24901 tgggtcacga tcaccatcgt catcccttct tcagccagat cctgcataac cttcagcact
    24961 tcatggcgca gttccgggtc aagagcggaa gtcggttcat caaacagcat cattttcggc
    25021 ttcaccgcca gcgcgcgggc aatcgccaca cgctgctgtt gaccaccaga aagttcggaa
    25081 gggtagtgat gtgcacgttc tgccagaccg actttcgcca gcagctcacg tgccagtttt
    25141 tccgcctctt ctttgttcgc gccacgcacg cgtagcggac caaacatgac gttttccagc
    25201 gccgtcagat gcgggaagag gtaaaactgc tggaacacca tacccgcttc ctggcgaatc
    25261 aggcgctcgt caactttcgg atcgttaacc ttcaggccat cgacaatcag atcgccggag
    25321 gtgatttctt ccagtttgtt gatgcagcgc agcagggtcg atttaccgga accggacggc
    25381 ccgataatca ccacgacttc gccctgagca atgttcaaat cgatattgtg cagcacctgg
    25441 gttggaccaa aatgcttgga gacgttttta aattcaatca caggattttc atccttcttt
    25501 ccagacgacg cagaataaag ctcagcacca gggtaataat cagatagaac accgccacgg
    25561 cgctccagat ctcaagggcg cggaagttac cggcaataat ttcttgcccc tgacgggtca
    25621 gttccgccac gccgataaca ataaacagcg aggtgtcttt aatgctgatg atccactggt
    25681 tacccagcgg cggcagcata cgacgcagtg ccagcggtaa aatgacgtag cgaatggttt
    25741 cccaacgtga aagaccgagc gccagtcctg cttcacgaaa acctttgtga atagacagca
    25801 ccgcaccacg cgtgatttcc gcaatgtacg cgccggagtt gatcatgatg gtgaccaccg
    25861 ccgcagtaaa tgggtcgatg cgtaagtcgt taaacgccat cggcagggcg aaatagataa
    25921 acatcacctg gacgacgata ggtgtgccgc ggatcacttc aataaagacc agcgcgacgt
    25981 ggttggctat ccaaccaccg aaggtgcgtg caaaacctgc cagcaatccg attaccagac
    26041 cgcctgccag accgaggacc gaaatccaca gggtcatttt ggcaccttca atcagaagcg
    26101 gaatggcagg ccagatggca ctccagtcaa actgcatatg ttgttcctgt taccgtggtg
    26161 taaataatca aattcagggt aattaatggc cccggagcgg gtttgcgccc ctcaccctaa
    26221 tcctctcccc ataggggaga gggaactgcc agtgcgtttt acaggtgtag cgttattatt
    26281 tcggttcagt accgaaccat tttttgtaga tttcgttgta agttccgttc tcgcgcaggg
    26341 ttttcaacgc gccgttgact ttgtcacgca gctcgtcgct acctttcggg aacgcaatac
    26401 cgtattgctg cgcttccaga gagtcaccta ccgctttgaa ctgaccgtta ccggcggttt
    26461 tgatgaagta cagaatgttt ggcgtatcgt gcagaacggc gtctgcgcgg ttggtgccca
    26521 gttccatata ggcgttatcg atgttcggga actgacgcag atctttagtt ttgatgtttg
    26581 ctttcgcgta atcaacggag ccagtaccgc tcttcacagc aaccactttc ccgtcgagat
    26641 ctttcacgct tttcacatcg ttattgttag ctttcaccat cactaacagg ccgcttttgt
    26701 agtagccgtc agagaaatcg atcgcttttt tacgctcgtc ggtgatggta atgcccgcca
    26761 gcgccagatc gacgtttttg gtttgcagtg ccggaatgat cccactgaaa tccatcggct
    26821 tcagttcgta atccagcttc agctctttag cgatggcagc ccacagatca acgtcaaagc
    26881 ccacatattt atcgccctgt ttaaattcaa acggaacgaa ggcggtatcc gtcgcgacaa
    26941 ctaatttttt atccgcggca tgagaagaaa ccgcaaaagc cagggtcagt gcagccagtg
    27001 aaacttttaa tacagacttc atagcatttc cttttttcta tccacgggac gatcccctgc
    27061 gtgacattca catatatgaa aaaatcgtgc cagttttgca actcattgat ttttcgagat
    27121 gcggcaaaaa cgtgatgcac gatttatagg gcaataccct gaagatgcac cattctgggg
    27181 caccaatctg gtgcgctaaa attgtgcact caacacagtg attatttagc gtaaaacctg
    27241 atgaaaaaac aatctttatg taacgattgt gtgatgatgt ggatacaaaa aatttactta
    27301 atcagctgga gatagcagat ggatgcacta aataagtgcg ttgaggtggg ctgcaaaaca
    27361 aaacggcctc ctgtcaggaa gccgctttta tcgggtacta aagttctgca ccatcagcga
    27421 tggatttatt cgatgttaga ctcgataaac cacaggaatt tatccaggtc gcgagacgcg
    27481 gcggtcagga tatctgcggt gtcgtcatct ttcgcttcgc caatcgcttt gcgtacgtca
    27541 ttagcgacga ttgcgtaacg gtcagccagt tctttcaggt gatcctgaac gttgtggatg
    27601 tccagcgggt aacttttcag cggggttttg ctgttgataa cttgagtggt ccccagagct
    27661 acaccgccca gctgcactgc acgttctgcc atggtatcca gatgatcgat cagtgcggtg
    27721 cggaagccat ccagcatttc atgtacggca atgaagttag cgccgcgcat gttccagtgc
    27781 gcttgtttgg taatcaaaga aagatcaata aactggataa cctggcgatt cagcaactct
    27841 actgttgctt ttttctcgct gtcggagaca tcgttgcggg tataaagcag attggtcgct
    27901 tttgatttaa ctaatttagc ggtactcata atttcatatc ctcttgatgt tatgtcccag
    27961 taattaacga gactaagtat agcaccggct atgtgttccg ctattctggc tgttcctatc
    28021 acactaatag tggtaacaag cgtgaaaaac aaaactaata aagattcaat gagttagata
    28081 tattgataag aacaattctt atttatctgc tcgccaggaa ttttcggaat aaatatgctt
    28141 ttgcataatt cgatatataa agacggtgta gaggaaaagt agcgagaaat tctgcatggt
    28201 tatgcataac catgcagaaa tgtaaattta attaatgtct aattctttta ttttgctctc
    28261 tttgcgtact gtcagcgtag aacccattga agcggcgata attgcgccga gcgccagcag
    28321 ctgtatgggt gtcagtgttt ctccgaggaa aatcatcccg gaaacggcag ccagcgccgg
    28381 ttccatgctc atcagcgtac caaatgtccg tgttggcaaa cgggtgaggg caatcatttc
    28441 cagcgaataa ggcagagcgg tcgagagaat agcgaccgcc agacccaatg gaataaccga
    28501 ccagtgccag agtgcttcac cagcctgaag cgctccaatt ggcacgaaaa ttaacgctgc
    28561 aatcaacgaa ccaattgcca ccgtcgcagg gccatgttcc gctcctgcgc gttgcccact
    28621 taaaatgtaa atagcccaac aagccccggc ccccagagcc agcgcacagc cggttaaatc
    28681 gacatgggaa acgtcttgcc ccagcggtag caggaaccac agaccaagaa ccgccagcac
    28741 aacccagacg aaatctaccg ggcgacgaga agagaacagc gccaccgcca gtggtccggt
    28801 gaactctagc gccaccgcaa tacccagcgg tactgtctga atagaaagat aaaaaagata
    28861 attcatccca cccagcgaaa cgccgtaaaa caacagcggt aaccgttgct ctttggcaaa
    28921 gcgcagtcgc catggcttaa agaacgcgat gaggatcagc gttcctaatg ccagacgcag
    28981 cgcagtgaca cccggtgcgc ccaccagagg aaaaagtgac ttagctaacg aggctccacc
    29041 ctgaatagac gccatggcaa cgagcaatat gactattggt aaccagaccg gcattttacg
    29101 caatgaacca ggcatccttt ctcccacaaa tatctagact taagtaaagc gtggagtgta
    29161 ctggatatac ccaatgctgg ttgagcattt gttgaaaaaa ttttcccccg ttttgactaa
    29221 aatgcgccag gattgatgga atcattagtc tggtgattag gaataatctg gatgaatgac
    29281 agggaaaaca tgcgtaatac ttacgcagtt ctctgaaaaa gtgatttaaa tttagatgga
    29341 tagcggtgta tggaaacgtt ctgttacatg aaatggcccg ttagacatca caaatcgcga
    29401 agagtttccc attaattttt gatatattta aaaattagga cttatttgaa tcacatttga
    29461 ggtggttatg aaaaaaattg catgtctttc agcactggcc gcagttctgg ctttcaccgc
    29521 aggtacttcc gtagctgcga cttctaccgt aactggcggt tacgcacaga gcgacgctca
    29581 gggccaaatg aacaaaatgg gcggtttcaa cctgaaatac cgctatgaag aagacaacag
    29641 cccgctgggt gtgatcggtt ctttcactta caccgagaaa agccgtactg caagctctgg
    29701 tgactacaac aaaaaccagt actacggcat cactgctggt ccggcttacc gcattaacga
    29761 ctgggcaagc atctacggtg tagtgggtgt gggttatggt aaattccaga ccactgaata
    29821 cccgacctac aaacacgaca ccagcgacta cggtttctcc tacggtgctg gtctgcagtt
    29881 caacccgatg gaaaacgttg ctctggactt ctcttacgag cagagccgta ttcgtagcgt
    29941 tgacgtaggc acctggattg ccggtgttgg ttaccgcttc taatcacttt ggtgatataa
    30001 aaaatccgcc tctcggggcg gatttttgtt tttaaggttt cgggtcgaaa atatcggttc
    30061 cgagatggtt gtaatccacc ttctgtaact ggaagttggt gatgtatatc ggcccggctt
    30121 tcttctcaga tataaacggg tatttgatat ttatttcctt cgctttaatt cccgtccact
    30181 gcgagaaaaa gcctaagaag tcattggctg agcggcgggc tttaatcacc cgatgcgctt
    30241 tatcgtcgct ggaaatgacc ataaaaggca cctggaaatt ttgctgatat ttatcatcat
    30301 gggcaaggta ttgcacgtct ttgccgcgct ctttaaaggc cagaccgtgg tcagaaaagt
    30361 aaaccagaga gaagctgctg ccgctgttgc gtaactgatc gtacagcttg cgcagtaaat
    30421 cgtccgtttg cgtcatggta tagagatagc acgacgtttc tttcgattgc acaaaggttt
    30481 cgtatttgcc ttgtgtcctg tcgcaggcct gcggatgcga gcccatcaga tgcagaacaa
    30541 tcagctgcgg ttgcgagtgc tcttgcgcca gcacttgagc ggtcatatcc agtaacgctt
    30601 cgtctttcgt gtttttatct gcttcaaaat taccttcttt caggaagtac acttcatctg
    30661 cacgtttggc gatgctggcg atagcagtat cgtattcgcc gatttggccc tggttggaaa
    30721 accaccaggt ctggaagccc gcgcgatttg ccagggtgac aaagttatcc tgaaactgtg
    30781 gtttgccatc gactacgcga ttgagcgtta agccaagcga tttctgcgtg gagccactgg
    30841 cggcaatgta gtcagcaaat atcaaaccgt taacgctgct ggcaaacggg gtattgtccc
    30901 agtgaccgcc aaaggcaccg agggcatcgc gacgcgcgct ttcaccgatc accaccacat
    30961 aggtctgata cttcggctta acggcagtga ccgtccaggt atctttcatt ccggaaagtt
    31021 ttgccatccg ttcttgctct tcaatcacct cgttattatt gacgatgaca tccttagcaa
    31081 aacgaaatac cggatagcca gtatctttca gtttaaatac gccaccccag gccaggtttt
    31141 gcacgggagc aacaaaaaat gtcgccacac tgaataccag acacaggctg tcgaaggtat
    31201 tccagcgcgc tttttcattc tctttttttc gcctgattgc tattacgccg agcgcaaaaa
    31261 taaataagcc gaccaggtag ctgtaccacg gaaaaatcgt caggatttcg gtcgactctt
    31321 ccatattggt ggagtgcaat gccagcaatg tattgaaatt aggcgcgccg taggcctgag
    31381 caaaagggaa ataacaagcc gccaccagcg aactgacacc gaccagaact ttttgtacgc
    31441 gaggcaatgt tcgccatagc aaaagcaaaa tagccgtaaa cgcagcggtg tagagcaaac
    31501 tgaaggggta acctaagccg aggttaatta atagcgactg taaaaagtag aacgcagtcc
    31561 acgggctaaa tacccggcta cgggtaacaa gcgattcttt gagggttaaa ttcatatgcc
    31621 actatcgtaa caaacgccat gtgcttaccc tggcgtcaag ggtcaatacc tgcaagagtg
    31681 cgtgaagagt ggaaaggggt ccgcatccgc gagccgcaat atacgcaggg ctgcaagaag
    31741 atagagcgag ccgccgttca ggtcaactac tgacgaacaa atcttttgcg aaggggattg
    31801 caaatccgac aaaaagtggg gtttttcaga ccaaaacgcg ttatgcctgc atgaaagcag
    31861 gagtgaagcg gcgtaccgcg acgccgctat ttatcggaag gtttatcttg ctgcggtttg
    31921 ttgttgacca tatcgcacaa catagagagc agcattaacc gtactttaaa gggagaatga
    31981 ctaaacacgc gcatacacct cttgaactca ttcataagac ctcctgactt gctaatcccg
    32041 tcgatccttg agggatgatt gcattacata cagatatagc acaggctata ttatatagct
    32101 attgctaaaa cgttaatttt ttgtaccttc gcaactctgg tttacaatgt gcgcacgaaa
    32161 tgagaacgct atgtatgcgt caccataatg aggaagcaca atgagtcgtc gcgcaggtac
    32221 gccaacagca aaaaaagtga cgcagttagt gaacgtggaa gagcacgttg aagggttccg
    32281 ccaggtcaga gaggcgcatc ggcgcgagct tattgatgat tacgttgagc tgatttctga
    32341 cttgatcagg gaagtggggg aagctcgtca ggtggacatg gctgctcgtc tgggagtttc
    32401 gcaaccgacg gtggctaaaa tgcttaagcg gctggcaacc atggggctga ttgaaatgat
    32461 cccctggcgt ggcgtgtttt taacggcaga aggagagaag ctggcgcagg aaagccgcga
    32521 gcgacatcag atagtcgaaa atttcttgtt ggtgttgggc gtcagtccgg aaatcgcccg
    32581 tcgcgacgcg gaaggcatgg agcaccatgt tagtgaagag acgctcgacg cttttcgttt
    32641 gtttacccag aaacacggtg ccaaatgagc ctgccttttt tacgcacgct gcaaggcgat
    32701 cgtttttttc agttattaat tcttgttggt atcggattaa gctttttcgt gccctttgca
    32761 ccgaaatcct ggcctgctgc tatcgactgg cacaccatca tcaccttaag cggcctgatg
    32821 ctgctgacca aaggtgtgga gttaagcggt tattttgatg tgctggggcg caaaatggtg
    32881 cgccgctttg ctacggagcg tcggctggcg atgtttatgg tgctggcggc ggcgctgctt
    32941 tctacctttc tgaccaacga tgtcgcgctg tttattgttg ttccgctgac tatcacgcta
    33001 aaaagactgt gtgagatccc ggttaatcgg ctgattattt ttgaggcgct ggcagtcaac
    33061 gctggttcgc tactgacgcc aattggcaac ccgcaaaata ttcttatctg gggacgttct
    33121 ggtctttcgt ttgccggatt tattgcccaa atggcaccgc tggctggcgc aatgatgctg
    33181 acgctcctgc tcctgtgctg gtgttgtttc cctggaaagg cgctgcaata ccatacgggg
    33241 gtgcaaacac cggagtggaa accgcggctg gtgtggagtt gtctggggct gtatatcgtc
    33301 tttctgacgg cgctggagtt caaacaagag ctgtggggac tggtgattgt ggcggcaggc
    33361 tttgcgctgc tggcacgtcg cgtggtgctc agtgtggact ggacgctgct gctggtgttt
    33421 atggcgatgt ttatcgacgt ccatttactg acccagcttc cagcgttgca aggcgtgttg
    33481 ggtaacgtga gtcatctatc tgaacccggg ttatggttaa cggcaatcgg tttatcgcag
    33541 gtgatcagta acgtgccgag taccatattg ttgctgaact atgtgccgcc gtctttatta
    33601 ctggtatggg cggtaaacgt aggtggcttt gggttattac ccggatcgct ggcaaatttg
    33661 attgcgctac gtatggcgaa cgatcgccgc atctggtggc gtttccatct ctattcaata
    33721 ccgatgctgt tgtgggcggc gttggtggga tatgttttgt tagttatact cccggccaac
    33781 tagggtctgg caaataaaaa aggcggatta tgagtccgcc ttttgcttta agttttgaag
    33841 attaattcag acgaaccggc atcccggagc ggtttttaat cgcttcatca agaacaacct
    33901 ggtcaacatc tggctgaccg gtcactgttt gcacgctctt cgtcagggta attggcacaa
    33961 tttcctgacc ttcaaactgg gcttcggtgg tagacagcgg gttatggact tcaatataac
    34021 ggctgccgtc tggctcggtg gtcgctttta ccggctcatc aataaactgt acgcgggtac
    34081 cgaccggtac tttctcgaac aggaatttga tgtcttcgtt acgcagacgc acacaaccat
    34141 gacttacacg caggccgata ccgaagttgg cgttggtgcc atggatagcg tacaggcgac
    34201 cgatgtagag tgcatacagc cccatcgggt tatccggacc tgccggaacg acagccggaa
    34261 gcggttcgcc cgcagcgcgg tactctgcgt gcattttggc ggtcggcgtc caggtcgggc
    34321 ctgctttctt acgctcaact ttggtggtcc agttgatagg cgtatctttg cctaactgac
    34381 caatgccgat cggcagcacg ataacggtgt tggtcccttt cggatagtaa tacagacgca
    34441 tctctgcact gttaatgacg atgccttcat gaacggtatc cggcaggatc agctgctgcg
    34501 gaatgttcag tacagtaccg cctttcggca ggaaggtatc cacacccggg ttcgcttcca
    34561 tcatattgga aagccccatc tggtactccg cggcaaaata ctccagcggc tgagtgttac
    34621 cttcaggaat ggtgatcacc tgattctgac caaccaggcg actcccgtcg gttggcagag
    34681 gataagttac cgcagaggcg gtactgcaaa agccgacaac agcgaaggcc gctgcgaata
    34741 atgttttcaa tttcatattc atgttaagcg agattttgtg cctggcaggc cattggattg
    34801 agaatattag agtattggaa gcgcattata agttcattcc agctcacagt gaaatcagat
    34861 gtgtacgaaa tcacattttt tgcctttggc ttgagtgtag accttaagcg aggagcagga
    34921 tcttctttca gacttatggc ataatgcgcg gtttgtcata tctcttttca ggatacgcct
    34981 gtgttagttt ccagtaacgt caccatgcag ttcggcagta agccgttgtt tgaaaacatt
    35041 tccgtcaaat ttggcggcgg caaccgttac ggcctgattg gcgcgaacgg tagtggtaaa
    35101 tccaccttta tgaagatcct cggcggcgac ttagagccga ctttgggtaa cgtttccctc
    35161 gatcccaacg agcgcattgg taagctgcgt caggatcagt ttgcctttga agagttcact
    35221 gtgctggata cggtgatcat ggggcataaa gagttgtggg aagtgaagca ggagcgtgac
    35281 cgcatctatg ctttgccgga aatgagtgag gaagacggct ataaagtggc cgatctggaa
    35341 gttaaatacg gcgaaatgga cggttactct gcggaagctc gcgccggtga attgttgctt
    35401 ggcgtgggaa ttccagtgga acagcactac ggcccgatga gtgaagttgc tcctggctgg
    35461 aagctgcgtg tgcttctggc gcaggcgctg tttgctgatc cggatattct cctgctcgac
    35521 gaaccgacca acaacctcga catcgacacc attcgctggc tggaacaggt gctgaacgag
    35581 cgtgacagca ccatgatcat catctcgcac gaccgtcact tccttaacat ggtctgtacg
    35641 cacatggcgg atctggatta cggcgagctg cgcgtttatc cgggtaacta cgatgagtac
    35701 atgacggcgg cgacccaggc gcgtgaacgt ctgctggccg ataacgccaa gaagaaagcg
    35761 cagattgctg agttgcaatc tttcgttagc cgctttagcg ccaacgcctc gaaatctcgc
    35821 caggcaactt cgcgcgcgcg ccagattgat aaaatcaaac tggaagaggt gaaagcctcc
    35881 agccgtcaga acccgttcat ccgttttgaa caggataaga aactgttccg taacgcgctg
    35941 gaagtggaag gtctgaccaa agggtttgat aacggtccac tgtttaaaaa tctcaacctg
    36001 ctgctggagg tcggtgaaaa acttgcggta ctgggtacca acggcgtcgg taaatcaacg
    36061 ctgctgaaaa cgctggtggg cgatctgcaa ccggacagcg gcaccgtaaa atggtctgaa
    36121 aacgcgcgca ttggttacta cgcccaggac cacgaatatg agtttgaaaa tgatctgacc
    36181 gtgttcgaat ggatgagtca gtggaagcag gaaggcgatg acgagcaggc ggtacgcagc
    36241 attctcggtc gtttgctgtt cagccaggac gacatcaaaa agccagctaa agtgctttcc
    36301 ggtggggaaa aagggcggat gctgtttggt aagttaatga tgcagaagcc gaacattctg
    36361 atcatggacg aaccgaccaa ccacctggat atggaatcca tcgagtcact gaacatggcg
    36421 ctggaactgt atcagggcac gctgatcttt gtttcacacg accgtgagtt cgtaagctcc
    36481 ctggcgaccc gcattctgga aatcaccccg gaacgcgtga tcgactttag cggtaattac
    36541 gaagattacc tgcgtagtaa agggatcgag taagtgatta tccgtcaccc cgatactgtc
    36601 ggggtgacgg cggctgatga caaatgcaaa atagcctgat gcgctacgct tatcaggcct
    36661 acgcagtacc tgtaatatat tgaatctgca ggactttgta ggccagataa ggcgttaacg
    36721 ccgcatcagg cattaagaaa gcgcacttgt cagcaatctg agtaacgtcg aatcgatttt
    36781 cttaatccca catcaattca cttccattaa ctctcaaaca tccatgccca acgctcgctt
    36841 accgtgaatg ttgaggtcgg caagcgtaaa gcgtccttcc cagtttgttt catagtcctc
    36901 gcgcgggaag tcgcccggcg atgcgccttt ttccagtgcg gccttcacct tgtgcgcgta
    36961 ggcaagattt ttctcgcaca tcggtgccgc aggaatgtac atcacgttgc cccaaccctg
    37021 ttgattttca acgggggcaa cggaatggat gacgtcgcag tgccaccaga cggagtctcc
    37081 ggcctcgagt tttggaatgc tggttaacgc ctcaattaac agtggatgcc attgctctga
    37141 taccggcaac actcttccgg gcgctacgcc gcacagttca tcctccggca catcatcaag
    37201 cagcggacgt aacagtacgt acgccatggc ttcaggaatg ggcacaacgt gcagcagccc
    37261 ctgaccaggc agcatatcag agagcgctgt ccagccctgg aatgtccgaa acacggaaca
    37321 tttggtggtg ttgtccaccg tgtactcttc aacttccgta cgatgtgccg catgccaggg
    37381 atcatattgc gccagattgc cattaaagac gttggcgaaa acgcgctgat acgctggaag
    37441 cagccagcgt tccagcgccc cggagtcggt atgcgctcca agacctttgg aggtcgttcc
    37501 gggcggacgg cggcggatac ggtcagggta gatgacgctc acatccgggt taaaccattg
    37561 ctttccatca ctttcaaatg tccacagacg attgagaaac gactgcgcat tcgccatttc
    37621 ttcactctgg cgggcctgca tttgcgcctg cgaccagtag atggggtaaa tctcgggacg
    37681 tgaagcgctg agcgtcccga agaaattatc gccggggcct ttgtagacct cgtcaaagcg
    37741 gttgcggtcc agatagtcca gcatcgactg atcccagcct agcgcttgtt cgcgggggaa
    37801 atggcctttt atcaccgcac aaccgcgacg tttaatctgt tcgcgctgct ctgcagtaac
    37861 atgacctgct ttgatatcgg cataagacag caccggccag acggcatcgc cctgtgcttt
    37921 gagtgcgttg atttcagcca ctcgcgtggc aatgtcatcg cttagctgat taaagatctg
    37981 ctggacgtcg ccaagctgcg cccgcagcgc gtgcttcatc tgacggatag ctgctttgtg
    38041 atcggcaggc aatgtgtcgc tggtaaaagt agaagccata accacctctc attaactttc
    38101 attcgaaacc attctgattt gcatgtgaca atataagtta taaataagtt aatgcaagtt
    38161 taatgatttg atgtgatgta caggatgtaa aaagaaggga aaaccggagc gcacggctcc
    38221 ggtagagagg tcagctgtta aaaggggatg tgttatccag caccgcctga atcacattca
    38281 gcgcgccttc atgattatta tcatcggtag cgtaacgggc gatttgttta atgttttccg
    38341 cagcattgcc catcgcaaag gaataacgcg ccattttcag catctccgca tcgttaccgc
    38401 tgtcgccaat cgctaccaca ttttgcggtg acagatccca gcgtttcagt aaccgcgaaa
    38461 taccgtttgc tttatgtaga ccgggaataa tcaggtcgat aaagccaaaa ccactggtga
    38521 cgggtttcat aatgccatcg agcgctatgt gcagtttgtc gatcactaac gggatttgtt
    38581 cgtctggcag gttgagcgaa aacttgaaca gtacgtcgtc aatctcctga taatctttta
    38641 cagctttcag gcgatggtag tgttttgcca tcagtgcgac aaatgcttcg ggggcatttt
    38701 cgctgacata tgcactttgc agaccgcagg cgacaaaatt gagttgctta tcttttagca
    38761 actcgccaat aacaatccgc gattcatgtc gggtcagttc gccgtggaac agttgcttgc
    38821 catgttcgta aaccagtgcg ccgttttccg cgacaaaaga gatctcatcc tttagctcag
    38881 gaaagaacga aataagctgg taatactgat taccgctggc gacaacgaac ttaatgccgc
    38941 gctttttcag ttcctgatat tgcgccataa aatgtggttg gttgtacgtt ttggcgtcgt
    39001 taagaaaagt accgtccatg tctgtgacga taacttttac gctcataggt gtgctcctgg
    39061 ctcgaaaatg aaaccgtaac agtgtaataa caatgtgacg cagagcacaa attatatttc
    39121 gaatgaaagt aaggatgaaa ttgatgatgt gaatgattta gcccggcgac gacgccgccg
    39181 ggccgaggag attacagcat atgttcagta cgggcgatga tatcgtcctg tgcgtctgga
    39241 gagagcgcgg tgaagaaggc ggaatagccc gcgacacgca ctaccagatc gcggtactga
    39301 tccggatgtt ttttcgcctc cagcagcgtt tcgcgggaaa cgatgttgta ctgaatatgc
    39361 cagcctttat gcacttcaaa gaaggtgcgc agcaggatca tcagtttctg cttgtcagat
    39421 tcgttctcca gcgttgccgg attcagtttc tggttgagca acacgccgcc gagaatcgct
    39481 gccgtaggca gtttacccac cgagccaatg accgcagtag ggccgagatg gtcagtacca
    39541 gaggccggac tagcgccttc tgccagcggg gtgtgggctt tacgcccgtc cggtgttgcc
    39601 atagtttgcg cgccaaacgg tacgttagcg gagatagacg aggtacccgc gtaatagttg
    39661 ccgccaaccg gaccacgacc gtagcgcggg ttatggtact gtttcagttc gtcgatatag
    39721 gtctgataag cgcgagccag cagcgtatcg acggtatcat cgtcgttgcc gtacttcggc
    39781 gcaccgttaa tcagccgctg acgcagttgc tcatgagtca ggccatcgaa gtcatccgcc
    39841 agtgcggcag ccagctgttg ctgaccaatc gcgccctgtt caaataccag tttcttcact
    39901 gccgccaggc tgttgccgag gttggcaatg ccaacctgca ggccagaaac ccagtcatat
    39961 ttcgcgccgc cttgcttgat acttttcgct cgctcaatac agtcatccac cagcgccgag
    40021 cagagaatat cgtgtacgtt ctcttccagc atggtgtcga cgacatattc gatttcgatt
    40081 gatttacgcg tgtaataacg gatttgcgta tcccacgcgt ccattacttc atcgaaattg
    40141 ttaaagttgc ctgccgacaa cgctttttct tgtggcagga acactttgcc gctggtggca
    40201 tcacgcccgc cttccagcgc cgccagcatc acgcgggcga agttgataaa gctcatgccg
    40261 gtacagcgat agccccattt gccaccgacg gcggtttcga tacaaccaat cgctgcgtag
    40321 tcgtaagcgt cctgcggttc aataccgagt ttaataaatt ccgggatcac gatttcgtcg
    40381 ttgttgaacg ccggcatccc gaagccgcaa cggatcacct gtacgcaggc gtcgaggaaa
    40441 tcattactca ttcctgcatg gtaacgcacg ctgaggttag gctgagtgga acgcaggcga
    40501 ccgcaggatt cgaggatcgc gtaagagagg ggattcactg catccattgg ttgaccatca
    40561 accagatttt gtccgccaat ggtaacgttc tgatacagcg gacttcccgc agaggctttt
    40621 gagtgtgagc cggagcggat cttgttcact tccagcagtt tcagccagca gctatgcagc
    40681 atctcgatgg cgtgttcgcg atccagcgtc tggttgagtt caacgtcgcg gcgatagtac
    40741 gggtagagat actggtccat acgaccaaac gataccgagt gaccgttaga ttcgatctgc
    40801 aaaatcaatt ggatgaagta acacagttgc agcgcctgcc agaaagtctg cggcggctgg
    40861 tgggcgataa gatcgcagtt ttctgccatc gtcagcagtt catcgcgacg gctttcgcga
    40921 gtttcggtag cagccatttc acgcgccagg gcagcgaaac gttcaatgtg ttcactgact
    40981 gccaccagca cgatatcaat cgctttcagg aattgttcgc catgtaaatc ttccagcacc
    41041 gtcagattga tgcgcgagcg acgttccgct actttctcac gcagaccatc aagacctttt
    41101 tccagcagca gcgggaaatt caccgccagg tgcgcatcgc cggaggtcat attgccttcc
    41161 gctttaatga ttccggtcgc cagcagacct ttttgctcat cggtaaacat gccgtagcag
    41221 cgatcctgta cggtctgacc gcgccaccac gggcacactt catgcagaac gcgtttgttc
    41281 tcttcgctca ccgcaaagcc agcaccggga cgatctgcca gatcatcaat ctctttttcg
    41341 atccacgaga cagtatattc cgggaagatc ggcgcggcgc gaacttcgct tgcctggttg
    41401 ccaatgatca actcatcgtg tttgatccag atggtgcgat tcgccaggtg atgcgccagt
    41461 gccagcgcgc gacgtaccgg gatcggctta tcgagatgtt gttgatacat ctcggtatag
    41521 tgctgcgcgc gctcggtaca gactggcggt ttcacaatat gcaccagcgc atttttgtgc
    41581 gctttaatgc ggtcgctgag cgtgtccagt ttcagtgtgg tcatggttgt tatcctcgta
    41641 aggtcgcggt taaccctttc tggcaggcat actgctgggc aaagtcgagc agttctggcg
    41701 catcaagcgg tttttccggg gcgtcatagg gcagattaag taagtgatat ttgttgatgc
    41761 ccagcgtgtg gtagggcaga aaatgaattt cgccaacgtg cagctcgtcg gcggcaaaat
    41821 cggtaatggc ttttacagag gtttcgtcgg cattaaagcc ctgaatcagc ggcacgcgga
    41881 taatgatttt tttgcccgct gcggcgagtt ttttcaggtt atccagcact ctggcggcgt
    41941 taccgtcggt ccactgttta aacggcgcgt cggcaacgtg ttttaaatcg gcaagaaaca
    42001 gatcgatata gggcagagaa ggggcgatat atttccacgg cacatgcaga caggtttcta
    42061 ccgcagtatg aatgcctgcc tcgtggctgg cttgcagtag cgccatcgcc atttccggct
    42121 gcataaaggg ctcaccaccc gaaagcgtta aaccgccgcc gctgcgatcg taaaacggtt
    42181 tatcgcgcag aacggtcgtc atgatctcct caacgctttt cacttcacca cacacggtta
    42241 atgcctgtgt cggacagcag tcggttaacg ccgtcagatg ctccggggtt aacttttccc
    42301 gatgaataag caaaccattc agcgcgcgct caatcacttc cggcgcggcc ttagcgcaca
    42361 gctcgcagcc ttccagacac agtcgtgcgt catacagcag atcctgcgtg cgggcgcggc
    42421 tttccgggtt ctgacaccag cggcagccca gcgaacagcc tttaagaaat acgaccgtgc
    42481 ggataccggg gccatcatgg gtcgagtagc gctgaatatt gaaaatcata gttgcctctc
    42541 tgtttcgttc aaacattaaa atactttcga atgaaagtta gattgatgtg cgtcaactgt
    42601 tcagagagtt ttcccgtgat agtctacatt cagacaaaaa gtacattttg aggatggtta
    42661 tggaactgta tctggatact tcagacgttg ttgcggtgaa ggcgctgtca cgtatttttc
    42721 cgctggcggg tgtgaccact aacccaagca ttatcgccgc gggtaaaaaa ccgctggatg
    42781 ttgtgcttcc gcaacttcat gaagcgatgg gcggtcaggg gcgtctgttt gcccaggtaa
    42841 tggctaccac tgctgaaggg atggtgaatg acgcacgtaa attgcgttct attattgcgg
    42901 atatcgtggt gaaagttccg gtgaccgccg aggggctggc agctattaag atgttaaaag
    42961 cggaagggat tccaacgctg ggaaccgcgg tatatggcgc agcacaaggg ctgctgtcgg
    43021 cgctggcagg tgcggaatat gttgcgcctt acgttaatcg tattgatgct cagggcggta
    43081 gcggcattca gactgtgacc gacttacacc agttattgaa aatgcatgcg ccgcaggcga
    43141 aagtgctggc agcgagtttc aaaaccccgc gtcaggcgct ggactgctta ctggcaggat
    43201 gtgaatcaat tacgctgcca ctggatgtgg cacaacagat gattagctat ccggcggtgg
    43261 aagccgctgt ggcgaagttt gaacaggact ggctgggagc gtttggcaga acgtcgattt
    43321 aaccggacgt tctgcatcct cataaattgc tgatgacgtg gcggagtgcc gcgtcttatc
    43381 tggcctggag gtggcaatta ctgcccgcac acctcacacc ccggattgcg catcagtttc
    43441 atttcgcgaa actgacaggt catcgcatcg tacatgacga ttttcccgct ggccggtttg
    43501 ccataacctg ccagcagttt gatcgcttcc attgcctgca acgaaccaat tacgccgatc
    43561 aacggtgcca tcacgcctgc ttctacgcag gttaatgcgt tctcgccaaa caaacggcta
    43621 aggcagcgat agcacggttc gccgtcctga taagtgaaga ccgtgatttg cccttccata
    43681 cgaattgccg cgccggaaac cagcggcacc ttcgcggcaa aacagccagc gttcagttga
    43741 ttacgcaccg caacgttatc cgtacagtcg agtaccagat cgtgttttgc aatcatcgca
    43801 gcaagttcag cgtcatcaag cagtgcattg actggcgtaa tcgcgatatg tgggttgatc
    43861 cgcgtcatgg cgtcacgggc ggattctacc ttcggttgcc cgaccgtagc atcactgtgc
    43921 agcgtctggc gttgcagatt cgagagcgaa accgtgtcga agtcgagcag cgtcaggttg
    43981 ccgacaccgg cgcttgccag atactgcgaa gctgcgcagc cgagtccacc gaggccgact
    44041 accagcacac gagaatcttt tagcgcctcc tgaccgtcaa aatcaaaacc gcgcagaatg
    44101 atttgccggt tgtagcgcag catctcctga tcgctgagtt ccgccattac aggcctccga
    44161 acaacgcgtt aaacggttct acttccaccc attcgcccac gtccacattg ccgcgatcgc
    44221 gttccagcac gataaagcag ttgccgagac taaaggagct aaatatatgt gaaccctgat
    44281 gtccggtggt cgtcacttca agttcaccgt cggcgttgcg ttgcagcacg ccgcgctgga
    44341 aatcaagacg tcctggcgtt tttttcaggc gggacgctgt gcgtacgcgc tggcgcgcgg
    44401 gcaggccgct ggcggtgttg ccgcttagtt ttgccagcaa aggctgtacc agttgataga
    44461 aggtcagcgt cgctgaaacc gggttgcccg gcaggccgca gaaccagcta ttgctgagtt
    44521 taccgaacgc gaacggttta cctggtttaa tcgccagctt ccagaaggcg atctccccca
    44581 gctcttcaag aatcgttttg gtgtaatccg cttcacccac tgaaacaccg ccggaactga
    44641 tcaccacatc cgcctggctg tcggcttcaa taaatgcggc gcgcagggca tgtggatcgt
    44701 cgcggataat ccctaagttg attacctcgc atcccaactg ttctaacatc aggtgtacgg
    44761 cgagacggtt ggtatcgtag atttggccat cgcccagtgg ctgaccgggc aactggagtt
    44821 catcaccggt agaaaaaagc gctacacgca ctttacgaat caccggaact tcggcaatcc
    44881 ccagtgaagc aatcactggc agttcagcgg tagtcaggcg agttcccgcc gggaaaacaa
    44941 ccgcacctgc agagatatct tcaccgcgac ggcgaatatt ttgcccgcta cgcacttcag
    45001 cagtaaaacg cacgccattg tccgtttgtt cagtctgctc ctgcatcacc accgcttcgc
    45061 agccttccgg caccggcgca ccggtcataa tacgaatgca ggtacccgca ggccattcac
    45121 catggtacgg ctgaccggca aaggatttac cggcaacggg cagcggttgc ctggaggcaa
    45181 tatcggctaa acgcaccgcg tagccgtcca ttgcggagtt atcaaacccc ggaacatcaa
    45241 gcggcgaaac gacatcgctc gccagaatac gaccaaaaca ctgtaccagt ggcagcgttt
    45301 cctgggcggt cagtggggtg acgcgagaaa gcatctcatt aagcgcggtg tcgagcgaca
    45361 tcaatccggt ggtaaattcc atgaaaacac tcctgcggag gcaaaatcga atttgcctat
    45421 tatgtcagaa aaacgccaca gactgtatgc cacctcgggc gtagcgttgg ctcctgcctt
    45481 tacatgccat atccatcttt ctatattcaa aaattgaatg agtaattcat aaaaattctg
    45541 atatttatag caaaagtggc gagccaccct taatggacga atactatggg caaagcagtc
    45601 attgcaattc atggtggcgc aggtgcaatt agccgcgcgc agatgagtct gcaacaggaa
    45661 ttacgctaca ttgaggcgtt gtctgccatt gttgaaaccg ggcagaaaat gctggaagcg
    45721 ggcgaaagtg cgctggatgt ggtgacggaa gcggtgcgtc tgctggaaga gtgtccactg
    45781 tttaacgccg gaattggcgc tgtctttacg cgtgatgaaa cccatgaact ggacgcctgt
    45841 gtgatggatg gtaacaccct gaaagccggt gcggtggcgg gcgttagtca tctgcgtaat
    45901 ccggttcttg ccgcccggct ggtgatggag caaagcccgc atgtgatgat gattggcgaa
    45961 ggggcagaaa attttgcgtt tgctcgtggc atggagcgcg tctcgccgga gattttctcc
    46021 acgcctttgc gttatgaaca actactggca gcgcgcaagg aaggggcaac cgtcctcgac
    46081 catagcggtg cgccactgga tgaaaaacag aaaatgggca ccgtgggggc cgtggcgttg
    46141 gatttagacg gcaatttggc ggcagccacg tccacaggcg gaatgaccaa taaattaccc
    46201 ggacgagttg gcgatagtcc cttagtgggt gccggatgct acgccaataa cgccagtgtg
    46261 gcggtttctt gtaccggcac gggcgaagtc ttcatccgcg cgctggcggc atatgacatc
    46321 gccgcgttaa tggattacgg cggattaagt ctcgcggaag cctgcgagcg ggtagtaatg
    46381 gaaaaactcc ctgcgcttgg cggtagcggt ggcttaatcg ctatcgacca tgaagggaat
    46441 gtcgcgctac cgtttaacac cgaaggaatg tatcgcgcct ggggctacgc aggcgatacg
    46501 ccaaccaccg gtatctaccg tgaaaaaggg gacaccgttg ccacacagtg atgaacttga
    46561 tgccggtaat gtgctggcgg ttgaaaatct gaatattgcc tttatgcagg accagcagaa
    46621 aatagctgcg gtccgcaatc tctcttttag tctgcaacgc ggtgagacgc tggcaattgt
    46681 tggcgaatcc ggctcctgta agtcagtgac tgcgttggca ttgatgcgcc tgttggaaca
    46741 ggcgggcggt ttagtacagt gcgataaaat gctgttgcag cggcgcagtc gcgaagtgat
    46801 tgaacttagc gagcagagcg ctgcacaaat gcgccatgtt cgcggtgcgg atatggcgat
    46861 gatatttcag gagccgatga catcgctgaa cccggtattt actgtgggtg aacagattgc
    46921 cgaatcaatt cgtctgcatc agaacgccag tcgtgaagaa gcgatggtcg aggcgaagcg
    46981 gatgctggat caggtacgca ttcctgaggc acaaaccatt ctttcacgtt atccgcatca
    47041 actctctggc gggatgcgcc agcgagtgat gattgcgatg gcgctgtcat gccgcccggc
    47101 ggtgctgatt gccgatgagc caaccaccgc gctggatgtc actattcagg cgcagatcct
    47161 gcaattaatc aaagtattgc aaaaagagat gtcgatgggc gttatcttta tcactcacga
    47221 tatgggcgtg gtggcagaga ttgccgatcg ggtactggtg atgtatcagg gcgaggcggt
    47281 ggaaacgggt accgtcgaac agatttttca tgcaccgcaa catccttaca cccgtgcgct
    47341 gttagctgct gttccgcaac ttggtgcgat gaaagggtta gattatcccc gacgtttccc
    47401 attgatatcg cttgaacatc cagcgaaaca ggcccccccc atcgagcaga aaacggtggt
    47461 ggatggtgaa cctgttttac gggtgcgtaa tctggtcacc cgtttccctt tgcgcagcgg
    47521 tttgttgaat cgcgtaacgc gggaagtgca tgccgttgag aaagtcagtt ttgatctctg
    47581 gcctggcgaa acgctatcgc tggtgggcga gtctggcagc ggtaaatcca ctaccgggcg
    47641 ggcgttgctg cgcctggtcg aatcgcaggg cggcgaaatt atctttaacg gtcagcgaat
    47701 cgataccttg tcacccggca aacttcaggc attgcgccgg gatattcagt ttatttttca
    47761 ggacccttac gcttcgctgg acccacgtca gaccatcggt gattcgatta tcgaaccgct
    47821 gcgtgtacac ggtttattgc caggtaaaga agcggctgca cgcgttgcgt ggttgctgga
    47881 gcgcgtgggc ctgttacctg aacatgcctg gcgttacccg catgagtttt ccggcggtca
    47941 gcgccagcgc atctgcattg ctcgcgcgtt ggcattgaat ccaaaagtga tcattgccga
    48001 cgaagccgtt tcggcgctgg atgtttctat tcgcgggcag attatcaact tgttgctcga
    48061 tctccagcgt gatttcggca ttgcgtatct gtttatctcc cacgatatgg ctgtggtaga
    48121 gcggattagt catcgtgtgg cggtgatgta tctcgggcaa attgttgaaa ttggtccacg
    48181 gcgcgcggtc ttcgaaaacc cgcagcatcc ttatacgcgt aaattactgg cggcagttcc
    48241 ggtcgctgaa ccgtcccgac aacgaccgca gcgtgtactg ctgtcggacg atcttcccag
    48301 caatattcat ctgcgtggcg aagaggtggc agccgtctcg ttgcaatgcg tcgggccggg
    48361 gcattacgtc gcacaaccac aatcagaata cgcattcatg cgtagataac attcaggcgg
    48421 agaataaaat ggcaagagct gtacaccgta gtgggttagt ggcgctgggc attgcgacag
    48481 cgttgatggc atcttgtgca ttcgctgcca aagatgtggt ggtggcggta ggatcgaatt
    48541 tcaccacgct cgatccgtat gacgcgaatg acacgttatc tcaggccgta gcgaaatcgt
    48601 tttaccaggg gctgttcggt ctggataaag agatgaaact gaaaaacgtg ctggcggaga
    48661 gttataccgt ttccgatgac ggcattactt acaccgtgaa attgcgggaa ggcattaaat
    48721 tccaggatgg caccgatttc aacgccgcgg cggtgaaagc gaatctggac cgggccagcg
    48781 atccggcgaa tcatcttaaa cgctataacc tgtataagaa tattgccaaa acggaggcga
    48841 tcgatccaac aacagtaaag attaccctca aacagccgtt ctcagcgttt attaatattc
    48901 ttgcccatcc ggcgaccgcg atgatttcac cggcagcgct ggaaaaatat ggcaaggaga
    48961 ttggttttca tccggtggga accggaccgt atgaactgga tacctggaat cagaccgatt
    49021 ttgtgaaggt gaaaaaattc gcgggttact ggcagccagg attgcccaaa ctggacagca
    49081 taacctggcg tccggtggcg gataacaaca cccgcgcggc aatgctgcaa accggtgaag
    49141 cgcagtttgc tttccccatt ccctacgagc aggccacact gctggagaaa aacaaaaata
    49201 tcgagttgat ggccagtccg tcaattatgc agcgttatat cagtatgaac gtgacgcaaa
    49261 agccgttcga taacccgaag gtccgtgagg cgctgaatta cgccattaac cgtccggcgc
    49321 tggtgaaagt tgcctttgcg ggctatgcaa cgccagctac tggtgtggta ccgccaagta
    49381 tcgcctacgc gcaaagttat aaaccgtggc cttacgatcc agtgaaagcg cgcgaattac
    49441 tgaaagaggc gggatatccc aacggtttca gtaccacgct gtggtcgtca cataaccaca
    49501 gcaccgcgca gaaagtgctg caatttaccc agcagcagtt agcgcaggtc gggattaaag
    49561 tccaggtgac tgcgatggat gccggacagc gggcggcaga agttgaaggt aaagggcaaa
    49621 aagagagcgg cgtgcggatg ttctacactg gctggtcggc ttcaaccggc gaagcggact
    49681 gggcgctatc gccactgttc gcctcgcaaa actggccacc gacgctgttt aataccgcat
    49741 tttacagcaa taaacaggtg gacgacttcc tggctcaggc actgaaaact aatgatccgg
    49801 cggaaaagac ccgcttgtat aaggcggcgc aggatatcat ctggcaagag tcgccgtgga
    49861 tcccgctggt ggtagaaaaa ctggtgtcgg cacacagtaa aaacctgacc ggtttttgga
    49921 tcatgccaga caccggcttc agctttgaag acgcggattt gcaataagca acgcagggag
    49981 tggaatgctt aattacgtta tcaaacgctt actggggttg attccgacgc tgtttatcgt
    50041 ctcggtgctg gtgtttttat ttgtgcatat gctgcccggc gatccggcgc gattgattgc
    50101 cgggcccgaa gctgatgcgc aggttataga actggtgcgt cagcagctgg ggttggatca
    50161 gccgctgtat caccagttct ggcactatat cagcaatgct gtgcaggggg attttggcct
    50221 gtcgatggtg tcgcgtcgtc cggttgccga tgagattgcc agccgcttta tgccaacgct
    50281 gtggctgacc ataaccagta tggtctgggc ggtgatattt ggtatggcgg cgggaattat
    50341 cgccgccgtc tggcgtaatc gttggccgga tcgattgagt atgaccattg cggtgtcggg
    50401 gatctcgttt ccggcatttg cgctggggat gcttttaatt caggtattct ccgttgaact
    50461 gggctggctg cctaccgtgg gagcagacag ttggcagcac tacattttac cctccctgac
    50521 gctcggcgcg gcagtggcag ccgtgatggc gcgctttacc cgcgcgtcgt ttgtcgatgt
    50581 tttaagcgaa gattatatgc gtaccgcgag ggcgaaaggg gtgagcgaaa cctgggttgt
    50641 cctcaaacac gggctacgta acgcgatgat cccggtagtg accatgatgg gcttacagtt
    50701 tggctttttg ctcggtggtt ctatcgtggt ggagaaagtt ttcaactggc ccggacttgg
    50761 acgcttactc gttgactccg tagaaatgcg cgattacccg gtgattcagg cggaaattct
    50821 gcttttctcg ctggaattta ttcttatcaa cttagtggtg gatgtgcttt acgccgccat
    50881 taacccggct atcaggtaca agtaaggatg cgactattta actggcgacg tcaggcggtg
    50941 ttaaacgcca tgccactggt caaacctgac caggtgcgta caccgtggca tgaattctgg
    51001 cgacgatttc gccgtcagca tatggcgatg accgccgcat tattcgttat tttattgatt
    51061 gtggtggcca tttttgcacg ctggatcgct ccctatgacg ccgaaaatta ttttgattat
    51121 gacaatctga ataacggacc ttctttgcag cactggtttg gcgtcgattc actggggcgc
    51181 gacattttca gccgtgtcct ggttggtgcg caaatctcgc tggcggcggg cgtgtttgcc
    51241 gtgtttatcg gtgcggcgat agggacgttg ctgggcttgc tggctggata ttatgaaggc
    51301 tggtgggatc ggctgatcat gcgcatttgc gatgtgctgt ttgccttccc gggtatttta
    51361 ctggcgatcg ctgttgttgc ggtgttggga agcggcattg ctaacgtgat tatcgcagtc
    51421 gccatttttt ccatccccgc gtttgcccgc ctggtgcgcg gcaacacgct ggtgttgaaa
    51481 cagcaaacct ttattgagtc agcacgcagt attggtgcca gcgatatgac cattttattg
    51541 cgtcatatct tgcctgggac cgtctcgtct atcgtggtgt ttttcaccat gcgcattggc
    51601 acctcgatta tctctgccgc cagcctgtca tttctcggcc tcggtgcgca gccgccgaca
    51661 ccagagtggg gagcaatgct caatgaggca cgagcggata tggttatcgc gccgcatgtc
    51721 gctgtttttc cggccctggc tatttttctg accgtactgg cgttcaattt gttgggcgat
    51781 ggtttacgcg atgcgctgga tccgaaaatt aaaggatagt tacgtttgaa tattgcttga
    51841 aagggtaatc acctcacagg caattattgc cctaagcaag tgttgtaact ttctgctgat
    51901 tttgtagaat cgggtaattt ggttaaaaag ccgcagcaag ggactatttt tgcagcggca
    51961 cagcgttcag atagttattt tgttaaatgt attaacatgc tgagtttata cgaaaagata
    52021 aagataaggc tgataatttt atttttattg gcagcactgt catttattgg tctttt
//
LOCUS       DBANUU010000012        51539 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000012 DBANUU010000000
VERSION     DBANUU010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.012"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(49..1413)
                     /gene="mnmE"
                     /locus_tag="ACIE68_000689"
     CDS             complement(49..1413)
                     /gene="mnmE"
                     /locus_tag="ACIE68_000689"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFX5907057.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(1519..3165)
                     /gene="yidC"
                     /locus_tag="ACIE68_000690"
     CDS             complement(1519..3165)
                     /gene="yidC"
                     /locus_tag="ACIE68_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFX5907058.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3168..3425)
                     /gene="yidD"
                     /locus_tag="ACIE68_000691"
     CDS             complement(3168..3425)
                     /gene="yidD"
                     /locus_tag="ACIE68_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFX5907059.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3389..3748)
                     /gene="rnpA"
                     /locus_tag="ACIE68_000692"
     CDS             complement(3389..3748)
                     /gene="rnpA"
                     /locus_tag="ACIE68_000692"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFX5907060.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(3765..3905)
                     /gene="rpmH"
                     /locus_tag="ACIE68_000693"
     CDS             complement(3765..3905)
                     /gene="rpmH"
                     /locus_tag="ACIE68_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFX5907061.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4135..4215)
                     /locus_tag="ACIE68_000694"
     CDS             complement(4135..4215)
                     /locus_tag="ACIE68_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5907062.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            4512..5915
                     /gene="dnaA"
                     /locus_tag="ACIE68_000695"
     CDS             4512..5915
                     /gene="dnaA"
                     /locus_tag="ACIE68_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFX5907063.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            5920..7020
                     /gene="dnaN"
                     /locus_tag="ACIE68_000696"
     CDS             5920..7020
                     /gene="dnaN"
                     /locus_tag="ACIE68_000696"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFX5907064.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7020..8093
                     /gene="recF"
                     /locus_tag="ACIE68_000697"
     CDS             7020..8093
                     /gene="recF"
                     /locus_tag="ACIE68_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFX5907065.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8122..10536
                     /gene="gyrB"
                     /locus_tag="ACIE68_000698"
     CDS             8122..10536
                     /gene="gyrB"
                     /locus_tag="ACIE68_000698"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFX5907066.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            10776..11174
                     /gene="yidB"
                     /locus_tag="ACIE68_000699"
     CDS             10776..11174
                     /gene="yidB"
                     /locus_tag="ACIE68_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFX5907067.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            11289..12101
                     /gene="yidA"
                     /locus_tag="ACIE68_000700"
     CDS             11289..12101
                     /gene="yidA"
                     /locus_tag="ACIE68_000700"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFX5907068.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12147..12803)
                     /gene="yidX"
                     /locus_tag="ACIE68_000701"
     CDS             complement(12147..12803)
                     /gene="yidX"
                     /locus_tag="ACIE68_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFX5907069.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13081..13770
                     /gene="dgoR"
                     /locus_tag="ACIE68_000702"
     CDS             13081..13770
                     /gene="dgoR"
                     /locus_tag="ACIE68_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFX5907070.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            13767..14645
                     /gene="dgoK"
                     /locus_tag="ACIE68_000703"
     CDS             13767..14645
                     /gene="dgoK"
                     /locus_tag="ACIE68_000703"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFX5907071.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            14629..15246
                     /gene="dgoA"
                     /locus_tag="ACIE68_000704"
     CDS             14629..15246
                     /gene="dgoA"
                     /locus_tag="ACIE68_000704"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFX5907072.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            15243..16391
                     /gene="dgoD"
                     /locus_tag="ACIE68_000705"
     CDS             15243..16391
                     /gene="dgoD"
                     /locus_tag="ACIE68_000705"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFX5907073.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            16466..17803
                     /gene="dgoT"
                     /locus_tag="ACIE68_000706"
     CDS             16466..17803
                     /gene="dgoT"
                     /locus_tag="ACIE68_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5907074.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17800..18864)
                     /gene="cbrA"
                     /locus_tag="ACIE68_000707"
     CDS             complement(17800..18864)
                     /gene="cbrA"
                     /locus_tag="ACIE68_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFX5907075.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            18965..20179
                     /gene="yidR"
                     /locus_tag="ACIE68_000708"
     CDS             18965..20179
                     /gene="yidR"
                     /locus_tag="ACIE68_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFX5907076.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(20181..20513)
                     /gene="yidQ"
                     /locus_tag="ACIE68_000709"
     CDS             complement(20181..20513)
                     /gene="yidQ"
                     /locus_tag="ACIE68_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFX5907077.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    20753..20825
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            20819..21232
                     /gene="ibpA"
                     /locus_tag="ACIE68_000710"
     CDS             20819..21232
                     /gene="ibpA"
                     /locus_tag="ACIE68_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFX5907078.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            21344..21772
                     /gene="ibpB"
                     /locus_tag="ACIE68_000711"
     CDS             21344..21772
                     /gene="ibpB"
                     /locus_tag="ACIE68_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFX5907079.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            21969..23630
                     /gene="yidE"
                     /locus_tag="ACIE68_000712"
     CDS             21969..23630
                     /gene="yidE"
                     /locus_tag="ACIE68_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFX5907080.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(23627..24343)
                     /gene="yidP"
                     /locus_tag="ACIE68_000713"
     CDS             complement(23627..24343)
                     /gene="yidP"
                     /locus_tag="ACIE68_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFX5907081.1"
                     /translation="MIYKSIAERLRIRPNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            24639..26255
                     /locus_tag="ACIE68_000714"
     CDS             24639..26255
                     /locus_tag="ACIE68_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFX5907082.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            26255..27069
                     /locus_tag="ACIE68_000715"
                     /pseudo
     CDS             26255..27069
                     /locus_tag="ACIE68_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27066..27959)
                     /gene="yidL"
                     /locus_tag="ACIE68_000716"
                     /pseudo
     CDS             complement(27066..27959)
                     /gene="yidL"
                     /locus_tag="ACIE68_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            28126..29841
                     /gene="yidK"
                     /locus_tag="ACIE68_000717"
     CDS             28126..29841
                     /gene="yidK"
                     /locus_tag="ACIE68_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFX5907083.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSIGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            29838..31331
                     /gene="yidJ"
                     /locus_tag="ACIE68_000718"
     CDS             29838..31331
                     /gene="yidJ"
                     /locus_tag="ACIE68_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFX5907084.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(31378..31827)
                     /gene="yidI"
                     /locus_tag="ACIE68_000719"
     CDS             complement(31378..31827)
                     /gene="yidI"
                     /locus_tag="ACIE68_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFX5907085.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            31937..32284
                     /gene="yidH"
                     /locus_tag="ACIE68_000720"
     CDS             31937..32284
                     /gene="yidH"
                     /locus_tag="ACIE68_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFX5907086.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            32274..32636
                     /gene="yidG"
                     /locus_tag="ACIE68_000721"
     CDS             32274..32636
                     /gene="yidG"
                     /locus_tag="ACIE68_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFX5907087.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            32633..33130
                     /gene="yidF"
                     /locus_tag="ACIE68_000722"
     CDS             32633..33130
                     /gene="yidF"
                     /locus_tag="ACIE68_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFX5907088.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(33138..34322)
                     /gene="emrD"
                     /locus_tag="ACIE68_000723"
     CDS             complement(33138..34322)
                     /gene="emrD"
                     /locus_tag="ACIE68_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFX5907089.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            34404..34478
                     /gene="ysdE"
                     /locus_tag="ACIE68_000724"
     CDS             34404..34478
                     /gene="ysdE"
                     /locus_tag="ACIE68_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFX5907090.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(34741..34830)
                     /gene="tisB"
                     /locus_tag="ACIE68_000725"
     CDS             complement(34741..34830)
                     /gene="tisB"
                     /locus_tag="ACIE68_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFX5907091.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            35395..35493
                     /gene="ivbL"
                     /locus_tag="ACIE68_000726"
     CDS             35395..35493
                     /gene="ivbL"
                     /locus_tag="ACIE68_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFX5907092.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            35599..37287
                     /gene="ilvB"
                     /locus_tag="ACIE68_000727"
     CDS             35599..37287
                     /gene="ilvB"
                     /locus_tag="ACIE68_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFX5907093.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            37291..37581
                     /gene="ilvN"
                     /locus_tag="ACIE68_000728"
     CDS             37291..37581
                     /gene="ilvN"
                     /locus_tag="ACIE68_000728"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFX5907094.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            37656..38246
                     /gene="uhpA"
                     /locus_tag="ACIE68_000729"
     CDS             37656..38246
                     /gene="uhpA"
                     /locus_tag="ACIE68_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFX5907095.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            38246..39748
                     /gene="uhpB"
                     /locus_tag="ACIE68_000730"
     CDS             38246..39748
                     /gene="uhpB"
                     /locus_tag="ACIE68_000730"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFX5907096.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            39758..41077
                     /gene="uhpC"
                     /locus_tag="ACIE68_000731"
     CDS             39758..41077
                     /gene="uhpC"
                     /locus_tag="ACIE68_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFX5907097.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            41215..42606
                     /gene="uhpT"
                     /locus_tag="ACIE68_000732"
     CDS             41215..42606
                     /gene="uhpT"
                     /locus_tag="ACIE68_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFX5907098.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(42651..44417)
                     /gene="adeD"
                     /locus_tag="ACIE68_000733"
     CDS             complement(42651..44417)
                     /gene="adeD"
                     /locus_tag="ACIE68_000733"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFX5907099.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQLLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            44592..45926
                     /gene="adeQ"
                     /locus_tag="ACIE68_000734"
                     /gene_synonym="yicO"
     CDS             44592..45926
                     /gene="adeQ"
                     /locus_tag="ACIE68_000734"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFX5907100.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            45979..46431
                     /gene="yicN"
                     /locus_tag="ACIE68_000735"
     CDS             45979..46431
                     /gene="yicN"
                     /locus_tag="ACIE68_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFX5907101.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            46642..47837
                     /gene="nepI"
                     /locus_tag="ACIE68_000736"
                     /pseudo
     CDS             46642..47837
                     /gene="nepI"
                     /locus_tag="ACIE68_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(47878..48171)
                     /gene="yicS"
                     /locus_tag="ACIE68_000737"
     CDS             complement(47878..48171)
                     /gene="yicS"
                     /locus_tag="ACIE68_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFX5907102.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            48393..49211
                     /gene="nlpA"
                     /locus_tag="ACIE68_000738"
     CDS             48393..49211
                     /gene="nlpA"
                     /locus_tag="ACIE68_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFX5907103.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(49215..50138)
                     /gene="yicL"
                     /locus_tag="ACIE68_000739"
     CDS             complement(49215..50138)
                     /gene="yicL"
                     /locus_tag="ACIE68_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFX5907104.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(50249..51433)
                     /gene="setC"
                     /locus_tag="ACIE68_000740"
     CDS             complement(50249..51433)
                     /gene="setC"
                     /locus_tag="ACIE68_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFX5907105.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARNSDKQGDRKLLILLCCLFGVLACTLFAWNS
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSIHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT        12005 a        13014 c        13854 g        12666 t
ORIGIN      
        1 tgaagcgaag ccgcatctga cagtcagaat acggcttcgt aagcgcggtt acttaccaat
       61 acagaagctg gagaaaatcc gccccagcag gtcgtctgaa gtaaattccc cggtgatttc
      121 gcttaagttc tgctgtgcca gacgcaactc ttccgccagc agttcacctg cccaggctcc
      181 caacagttgc gctttgccct gttgtagatg ttccgctgcc tgttccagcg cctgtaggtg
      241 gcgacgacgc gccaggaagc cgccttccat gttggtgtca aagcccatgc tctgtttgag
      301 atggttacgc agcacgtcca cgccttcacc agtccttgcc gagagacgaa ttaacgcgtg
      361 accgttcact tcactcattc ccagcgtttc gccggtgata tcggctttat tgcgcaccac
      421 ggtgatcggc agtttcgctg gcagacgggc aataaattcc ggccagatct ctgccggatc
      481 cacggcgtct gttgtggtgc catcgaccat aaacagcacg cggtcggcct gttcaatttc
      541 ctgccacgcg cgctcgatac caatacgttc tacttcgtca ctggcttcac gtagcccggc
      601 ggtatcgatg atatgcagcg gcattccgtc aatgtggata tgctcacgca gcacgtcacg
      661 cgtagttccg gcgatatcgg ttacgattgc cgcttcacgc cccgccagcg cgtttaacag
      721 gctcgattta ccggcgttag gacgtccggc aatcaccact ttcatccctt cgcgcaacaa
      781 actaccctga cgtgcttcag cacgcactgc atcaagatcg gcaataacgt cattgagctg
      841 ggcttcaatt tttccgtcgg agaggaaatc gatctcttca tcggggaaat caattgccgc
      901 ttcgacgtaa atgcgcaagt gggtgagggc ttctaccaga tgattaaccc gtgcggagaa
      961 tgcgccttgc agcgagttaa gtgccgaacg ggccgcctgt tccgaactgg cgtcgataag
     1021 atcggcaatc gcctcggcct gggctaagtc aagtttatcg ttaagaaacg cgcgttcgga
     1081 aaactcacca gggcgagcaa tccgcaggcc gggaatggtc agaatgcgtt ttaacagcag
     1141 gtcgaggatc accggaccgc catgaccttg cagttccagc acatcttcgc cggtgaacga
     1201 gttcgggcca gggaaccata gcgcaatccc ctgatcgagc acgctgccgt cggcgtcttt
     1261 aaacggaaga taatcggcgt agcgcggctt aggcagttta cccagcacgg tttcggcaac
     1321 ttcacgggct ttgaagccgg agatgcgcag gatgccaacg ccgccacgtc ccggaggcgt
     1381 ggcctgggct acgatagtgt cattatcgct catgatgttc ctgttgcttt gtgtggcgga
     1441 tgcgcggtgc ttatccgccc tacgaaaaga aaaaaggcgg tcaactgacc gcccttattt
     1501 tagcgaaaac tcaccgaatc aggatttttt cttctcgcgg ctatgcaggc cacgtttttc
     1561 cagaccacgg taaatcagct gctgctgaat aatggttacc aggttgctga cgatatagta
     1621 cagcaccaga cctgacggga accacaggaa gaacacggtg aagatgaccg gcataaaggt
     1681 catgatcttc tgctgcatcg ggtcggtcac tgtggtcggc gacatcttct gaatgaagaa
     1741 catcgttacg cccatcagga tcggcaggat gtagtacggg tcctgtgccg acaggtcgtg
     1801 gatccacagt gcaaacggtg cctgacgcag ttcaacggaa cccatcagca tgtagtacaa
     1861 cgccaggaag attggcatct ggatcagcag cgggaagcag ccgcccagcg ggttaacctt
     1921 ctcagctttg tacagcgcca tcatttcctg gctgatacgc tgtttgtcat cgcccagacg
     1981 ctcacgcatt gcctgaatct tcggctgcag catacgcatc ttcgccatgg aggtgtactg
     2041 cgctttggtc agcgggtaca tgatgccacg aacgataaag gtgatgatga taatggagaa
     2101 gccccagtta cccacaaagc tatggatcca tttcagcagt ttgaacagcg gctgagagat
     2161 gaaccacaac caaccgtaat caacggtcag atccaggtgc ggagcaacag ctgccatttt
     2221 gtcctggatt tccgggccaa cccacagggt gctgttcatc gcgccagtct gaccaggctg
     2281 aaccagtacc ggctgagatt tatagccgat agcggcgatg ccgttaccca gattagcggt
     2341 atagaagttg ttggtaccgt cgttatgcgg gatccacgcc gtcgcgaaat actgttgcag
     2401 catcgccacc caaccacctt tcgaagagat gttcaggttt tcgttatcgg caatggtatc
     2461 gaacttgtat ttctcatact tctcgtcagg cgtggagtac gccgcgccac ggaaggtgtg
     2521 cagtgcgaag ttgctgcttc cggtatcgag atgcggtggc agagtgatgg attgcttcaa
     2581 ctgaccaaag gtggagattt ccagcggttt ctcgccagcg ttctgcacgt tgtagttgac
     2641 gttgacagcg taatcaccac gtttcaggac aaacgttttg gtaaacgtgt tgcctgccgc
     2701 gtcggtatac gtcatcggca cctgcagttc gttttgacct tcagccagca cataagcgtc
     2761 tttttcaacg ttatacagcg gacgcgggcc gttagccggg ttatccgggc catcacgacc
     2821 ggtcagaccg ctctgtgcct gataaataaa ctgcggtgaa gtttccagca gctggaacgg
     2881 ctgggtagag ttcagctctt tcgggtaagc cggcagcaga gcttgctcaa catcaccacc
     2941 acgggtgttg atggtcagat caagcacgtc ggtcttaacc gagatcagtt tcccctggcc
     3001 actggccggt acgccctggt cggcggcgct acccgctgcg gtggtcgttg tctgcgtggt
     3061 ctgttgggcc tgaggttgcg ggtttttatc ctgctcccag gcttgccaga tcatgaaaga
     3121 cacgaacagc aaagcgatga ctaaaagatt gcgttgcgaa tccatcgtta gtgttctctg
     3181 gtatcaaatg gtccgggcgg gacgggatcg tcaccaccag ggtgtaaagg gtggcatttt
     3241 aatacgcgtt tcaccgtcaa ccaactgcct tttatcactc caaacctgcg caatgcctca
     3301 attccgtagc ttgaacaggt tggagtgaaa cgacaatgcg gcccgagtag cggactaatc
     3361 aggcgttgat agacccgaat gagggctatc aggacccgcg agccaggcga cagtggcggc
     3421 gccataattt ttccaacgct tccgagagag cacggttatc gaggtcggca accccttttt
     3481 tcgccaccac cacgaaatcc atagccggga gttcatgttg gcgcagacgg aagctttcac
     3541 gcgtcagacg tttaatccga ttgcgttcat gggcgcgtcg aacgtttttc ttggcgactg
     3601 taagaccgat acggggatgc cccagcgaat tcaggcggcc gagaatggta atttgcggcg
     3661 tgccagcccg ttgtggctgc tggaagacga atgtgaattg actgggagtt aacaagcgta
     3721 actccctggg aaatgcgagc ttaaccactc aggggttagc tttattactt agaaacggtc
     3781 agacgagcgc ggcctttagc acgacgacgt gccagaacct gacgaccatt tttagtagcc
     3841 atacgagcac ggaagccgtg agaacggttg cgcttcagta cagacggttg aaaagtgcgt
     3901 ttcatggcga tttctaccta aacttgaata aattcaatgg ctttattgga tatccgccga
     3961 aaaatgaaac gatggacacc gaagccatgg gtgattaaag aggccggatt gtaataattg
     4021 tacactccgg agtcaattct ctttccttat ttaccgcgct tttccgcacc ttttcgcagg
     4081 gaaaatgtac gacctcacac cagtgaaaac cagcatggcg cgccgggtgg aggattatac
     4141 gggctgatgg gtaaagcgca aggatcgtcc tggatcttta ttagatcgat taagccaatt
     4201 tttgtctatg gtcattaaat tttccaatat gcggcgtaaa tcgtgcccgc ctcgcggcag
     4261 gatcgtttac acttagcgag gtctggaaag tcctgtggat aaatcgggaa aatctgtgag
     4321 aaacagaaga tctcttgcgc agtttaggct atgatccgcg gtcccgatcg ttttgcagga
     4381 tcttgatcgg gcatataacc gcagacagcg gttcgtgcgt caccctcaag cagggtcttt
     4441 tcgacgtacg tcaacaatca tgaatgtttc agccttagtc attatcgact tttgttcgag
     4501 tggagtccgc cgtgtcactt tcgctttggc agcagtgtct tgcccgattg caggatgagt
     4561 taccagccac agaattcagt atgtggatac gcccattgca ggcggaactg agcgataaca
     4621 cgctggccct gtacgcgcca aaccgttttg tcctcgattg ggtacgggac aagtacctta
     4681 ataatatcaa tggactgcta accagtttct gcggagcgga tgccccacag ctgcgttttg
     4741 aagtcggcac caaaccggtg acgcaaacgc cacaagcggc agtgacgagc aacgtcgcgg
     4801 cccctgcaca ggtggcgcaa acgcagccgc aacgtgctgc gccttctacg cgctcaggtt
     4861 gggataacgt cccggccccg gcagaaccga cctatcgttc taacgtaaac gtcaaacaca
     4921 cgtttgataa cttcgttgaa ggtaaatcta accaactggc gcgcgcggcg gctcgccagg
     4981 tggcggataa ccctggcggt gcctataacc cgttgttcct ttatggcggc acgggtctgg
     5041 gtaaaactca cctgctgcat gcggtgggta acggcattat ggcgcgcaag ccgaatgcca
     5101 aagtggttta tatgcactcc gagcgctttg ttcaggacat ggttaaagcc ctgcaaaaca
     5161 acgcgatcga agagtttaaa cgctactacc gttccgtaga tgcactgctg atcgacgata
     5221 ttcagttttt tgctaataaa gaacgatctc aggaagagtt tttccacacc ttcaacgccc
     5281 tgctggaagg taatcaacag atcattctca cctcggatcg ctatccgaaa gagatcaacg
     5341 gcgttgagga tcgtttgaaa tcccgcttcg gttggggact gactgtggcg atcgaaccgc
     5401 cagagctgga aacccgtgtg gcgatcctga tgaaaaaggc cgacgaaaac gacattcgtt
     5461 tgccgggtga agtggcgttc tttatcgcca agcgtctacg atctaacgta cgtgagctgg
     5521 aaggggcgct gaaccgcgtc attgccaacg ccaactttac cggaagggcg atcaccatcg
     5581 acttcgtgcg tgaggcgctg cgcgacttgc tggcattgca ggaaaaactg gtcaccatcg
     5641 acaatattca gaagacggtg gcggagtact acaagatcaa agttgcggat ctcctttcca
     5701 agcgtcgatc ccgctcggtg gcgcgtccgc gccagatggc gatggcgctg gcgaaagagc
     5761 tgactaacca cagtctgccg gagattggcg atgcgtttgg tggtcgtgac cacacgacgg
     5821 tgcttcatgc ctgccgtaag atcgagcagt tgcgtgaaga gagccacgat atcaaagaag
     5881 atttttcaaa tttaatcaga acattgtcat cgtaaaccta tgaaatttac cgtagaacgt
     5941 gagcatttat taaaaccgct acaacaggtg agcggtccgt taggtggtcg tcctacgcta
     6001 ccgattctcg gtaatctgct gttacaggtt gctgacggta cgttgtcgct gaccggtact
     6061 gatctcgaga tggaaatggt ggcacgtgtt gcgctggttc agccacacga gccaggagcg
     6121 acgaccgttc cggcgcgcaa attctttgat atctgccgtg gtctgcctga aggcgcggaa
     6181 attgccgtgc agctggaagg tgaacggatg ctggtacgct ccgggcgtag ccgtttttcg
     6241 ctgtctaccc tgccagcggc ggatttcccg aacctcgatg actggcagag tgaagtcgaa
     6301 tttaccctgc cgcaggcaac gatgaagcgt ctgattgaag cgacccagtt ttctatggcg
     6361 catcaggacg ttcgctatta cttaaatggt atgctgtttg aaaccgaagg tgaagaactg
     6421 cgcaccgtgg caaccgacgg ccaccgtctg gcggtctgtt caatgccaat tggtcaatct
     6481 ttgccaagcc attcggtgat cgtaccgcgt aaaggcgtga ttgaactgat gcgtatgctc
     6541 gacggcggcg acaatccgct gcgcgtacag attggcagca acaacattcg cgcccacgtt
     6601 ggcgacttta tcttcacctc caaactggtg gatggtcgct tcccggatta tcgccgcgtt
     6661 ctgccgaaga acccggacaa acatctggaa gctggctgcg atctgctcaa gcaggcgttt
     6721 gctcgcgcgg cgattctctc taacgagaaa ttccgcggcg tacgtcttta tgtcagcgaa
     6781 aaccagctga aaatcaccgc caacaacccg gaacaggaag aagcggaaga gatcctcgac
     6841 gttacctata gtggtgcgga gatggaaatc ggcttcaacg tcagctacgt gctggatgtt
     6901 ctgaacgcgc tgaaatgtga aaacgtccgc atgatgctga ccgattcggt ttccagcgtg
     6961 cagattgaag atgccgcatc acagtcggct gcctatgttg tcatgccaat gagactgtaa
     7021 tgtccctcac ccgcttgttg atccgcgatt tccgcaacat tgaaaccgcg gatctcgctt
     7081 tatctcccgg ctttaacttt ctggtaggtg ccaacggcag tggcaaaacc agcgtgctgg
     7141 aagccatcta tacgctcggc catggtcggg cgtttcgcag tttgcagatt ggtcgcgtca
     7201 ttcgccatga gcaggaggcg tttgttctcc acgggcgatt acagggcgaa gagcgcgaga
     7261 cagcgattgg cttaaccaaa gacaaacagg gcgacagcaa agtccgcatc gacggtacag
     7321 acgggcataa ggtcgcggaa ctggcgcacc tgatgccaat gcagttgata acgccagaag
     7381 ggtttacttt actcaacggc ggccccaaat acagaagagc attcctcgac tggggatgct
     7441 ttcacaacga acccggattt ttcaccgcct ggagcaatct caagcgattg ctcaagcagc
     7501 gcaatgcggc gctgcgccag gtgacacgtt acgaacagct acgcccgtgg gataaagaac
     7561 tgatcccgct ggcggagcaa atcagcacct ggcgcgcgga gtatagcgcc ggtatcgcgg
     7621 ctgatatggc cgatacttgt aagcaatttc tccctgagtt ttctctgact ttctctttcc
     7681 agcgcggctg ggagaaagag acagaatatg ctgaggtgct ggaacgtaat tttgaacgcg
     7741 atcgccagct aacctacacc gcgcatggcc cgcataaagc ggacttacgc attcgcgccg
     7801 acggtgcgcc ggtggaagat accttatcgc gtgggcagct taagctgttg atgtgcgcct
     7861 tacgtctggc gcaaggagag ttcctcaccc gtgaaagcgg gcggcggtgt ctctacctga
     7921 tagatgattt tgcctctgag cttgatgatg agcgtcgcgg gctgcttgcc agccgcttaa
     7981 aagcgacgca atcacaggtc tttgtcagcg cgatcagtgc tgaacacgtt atagacatgt
     8041 cggacgaaaa ttcgaagatg tttaccgtgg aaaagggtaa aataacggat taacccaagt
     8101 ataaatgagc gagaaacgtt gatgtcgaat tcttatgact cctccagtat caaagtcctg
     8161 aaagggctgg atgcggtgcg taagcgcccg ggtatgtata tcggcgacac ggatgacggc
     8221 accggtctgc accacatggt attcgaggtg gtagataacg ctatcgacga agcgctcgcg
     8281 ggtcactgta aagaaattat cgtcaccatt cacgccgata actctgtctc tgtacaggat
     8341 gacgggcgcg gcattccgac cggtattcac ccggaagagg gcgtatcggc ggcggaagtg
     8401 atcatgaccg ttctgcacgc aggcggtaaa tttgacgata actcctataa agtgtccggc
     8461 ggtctgcacg gcgttggtgt ttcggtagta aacgccctgt cgcaaaaact ggagctggtt
     8521 atccagcgcg agggtaaaat tcaccgtcag atctacgaac acggtgtacc gcaggccccg
     8581 ctggcggtta ccggcgagac tgaaaaaacc ggcaccatgg tgcgtttctg gcctagcctc
     8641 gaaactttca ccaatgtgac cgagttcgaa tatgaaattc tggcgaaacg tctgcgtgag
     8701 ttgtcgttcc tcaactccgg cgtttccatt cgtctgcgcg acaagcgcga cggcaaagaa
     8761 gaccacttcc actatgaagg cggcatcaag gcgttcgttg aatatctgaa caagaacaaa
     8821 acgccgatcc acccgaatat cttctacttc tccactgaaa aagacggtat tggcgtcgaa
     8881 gtggcgttgc agtggaacga tggcttccag gaaaacatct actgctttac caacaacatt
     8941 ccgcagcgtg acggcggtac tcacctggca ggcttccgtg cggcgatgac ccgtaccctg
     9001 aacgcctaca tggacaaaga aggctacagc aaaaaagcca aagttagcgc caccggtgac
     9061 gatgcgcgtg aaggcctgat tgcggtcgtt tccgtgaaag tgccggaccc gaaattctcc
     9121 tcccagacca aagacaaact ggtttcttct gaggtgaaat cagcggttga acagcagatg
     9181 aacgaactgc tggcagaata cctgctggaa aacccaaccg acgcgaaaat cgtggttggc
     9241 aaaattatcg atgctgcccg tgcccgtgaa gcggcgcgtc gcgcgcgtga aatgacccgc
     9301 cgtaaaggtg cgctcgactt agcgggcctg ccgggcaaac tggcagactg ccaggaacgc
     9361 gatccggcgc tttccgaact gtacctggtg gaaggggact ccgcgggcgg ctctgcgaag
     9421 caggggcgta accgcaagaa ccaggcgatt ctgccgctga agggtaaaat cctcaacgtc
     9481 gagaaagcgc gcttcgataa gatgctctct tctcaggaag tggcgacgct tatcaccgcg
     9541 cttggctgtg gtatcggtcg tgacgagtac aacccggaca aactgcgtta tcacagcatc
     9601 atcatcatga ccgatgcgga cgtcgacggc tcgcacattc gtacgctgct gttgaccttc
     9661 ttctatcgtc agatgccgga aatcgttgaa cgcggtcacg tctacatcgc tcagccgccg
     9721 ctgtacaaag tgaagaaagg caagcaggaa cagtacatta aagacgacga agcgatggat
     9781 cagtaccaga tctctatcgc gctggatggc gcaacgctgc acaccaacgc cagcgcaccg
     9841 gcattggctg gcgaagcgtt agagaaattg gtgtctgagt acaacgcgac gcagaaaatg
     9901 atcaaccgca tggagcgtcg ttatccgaaa gcaatgctga aagagcttat ctatcagccg
     9961 acgctgacgg aagccgacct ctctgatgag cagaccgtta cccgctgggt gaacgcgctg
    10021 gtcagcgaac tgaacgacaa agaacagcac ggcagccagt ggaagtttga tgtccacacc
    10081 aatgccgaac aaaacctgtt cgagccgatt gttcgcgtgc gtacccacgg tgtggatact
    10141 gactatccgc tggatcacga gtttatcacc ggtggcgaat atcgtcgtat ctgcacgctg
    10201 ggtgagaaac tgcgtggctt gctggaagaa gatgcgttta tcgaacgtgg cgagcgtcgt
    10261 cagccggtag ccagcttcga gcaggcgctg gactggctgg tgaaagagtc ccgtcgcggc
    10321 ctctccatcc agcgttataa aggtctgggc gagatgaacc cggaacagct gtgggaaacc
    10381 accatggacc cggaaagccg tcgtatgctg cgcgttaccg ttaaggatgc gattgccgct
    10441 gaccagttgt tcaccacgct gatgggcgac gccgttgaac cgcgccgtgc gtttattgaa
    10501 gagaacgccc tgaaagcggc gaatatcgat atttaatggc gctaaccatg cgagcgtgcc
    10561 tgatgcgcta cgcttatcag gcctacgaaa atcttgcaat gtattgaatt ttcatgattt
    10621 tgtaggccgg ataaggcgtt cacgccgcat ccggcaagaa gctataagaa aagggcggag
    10681 atcatctccg ccctttttat ttctgcaatc cgcaattatc atcccgcaaa tctatacttt
    10741 ttgggtcaac acgtaacgct ttacaggtga ttatcatggg gctttttgat gaagttgtcg
    10801 gtgcctttct gaaaggcgat gcggggaaat atcaggctat tttaagttgg gttgaggagc
    10861 agggcggcat tcaggtgctg ctggaaaaac tgcaaagtgg cggcttaggg gccattctct
    10921 caacctggct gagtaatcaa cagggcaatc aatcggttag tggcgagcaa ctggaatcgg
    10981 cgctcggcac aaatgcggtg tccgatcttg gacaaaaact tggcgtggat accagtacag
    11041 cttccagttt actggcagaa caattgccga agattattga tgcgctctca ccgcaaggtg
    11101 aagtgtctcc acaagccaat aacgatctgc tttccgcagg catggaactg ctgaaaggga
    11161 aactcttccg ctaagcaaat ggggagcatg caacccgcgt tgcatccccc ttttccctcg
    11221 ggtgttgtac tgatttttga gcggaatcgc gttagcatgg gtcaggaact aatctacctg
    11281 gggaactcat ggctatcaaa ctcattgcta tcgatatgga tggcaccctt ctgctgcccg
    11341 atcacaccat ttcacccgcc gttaaaaatg cgattgccgc agctcgcgcc cgtggcgtga
    11401 atgtcgtgct aacgacgggt cgcccgtatg caggtgtgca caactacctg aaagagctgc
    11461 atatggaaca gccgggcgac tactgcatta cttataacgg cgcgctggta cagaaggccg
    11521 ctgatggtag caccgtggcg caaactgctc tcagctatga cgactatcgt ttcctggaaa
    11581 aactctctcg cgaagtcggt tctcatttcc acgccctgga ccgcaccacg ctgtacaccg
    11641 ccaaccgtga tatcagctac tacacggtgc atgaatcctt cgttgccacc attccgctgg
    11701 tgttctgcga agcggagaaa atggacccca atacccagtt cctgaaagtg atgatgattg
    11761 atgaacccgc catcctcgac caggctatcg cgcgtattcc gcaggaagtg aaagagaaat
    11821 ataccgtgct gaaaagtgcg ccgtacttcc tcgaaatcct cgataaacgc gttaacaaag
    11881 gtacgggggt gaaatcactg gccgacgtgt taggtattaa accggaagaa atcatggcga
    11941 ttggcgatca ggaaaacgat atcgcaatga ttgaatatgc aggcgtcggt gtggcgatgg
    12001 ataacgctat tccttcggtg aaagaagtgg cgaactttgt caccaaatcc aaccttgaag
    12061 atggcgtggc gtttgctatt gagaagtatg tgctgaatta atcagtggac gggcaaacag
    12121 cataaacgcg tttgcccgct tactgattat tggtttactg catatacaac cagaaattgc
    12181 agcggtgcag taatgatgtc cagcgtaaca gtcactggca gtaatgctgc ggaaacacca
    12241 tccggaatgc ctctgggtga atagtattca taaaatcgca ctttaaacgg atgatagaac
    12301 gccatcacct ttgagtaatc catgttttta tttttctcat ggattgtgcc ttttaggttt
    12361 tccagtgacc tggtacagtg ttgaacacca caggcaaaac cataatgcgt tgccagtgcc
    12421 ttttcttctt cgttattcca gtagtagtaa agcgatattg tgccggtaaa tttctttttc
    12481 cccgcattta aaacaaatct tgcgtcatcg gcaacctgaa tattgtgccg gttaagtgct
    12541 ggatcattga gcatcttaac gatgtcatcg gcaccatcag tgataaggta atccagcgat
    12601 tcccctacaa aaacatagcc ttttgttcca ttactatctt ttgcctgcga tagcccagtg
    12661 atggtgtctg actgcgattg atactttcct gaactaccct ttgcggtatg ggaaaccaga
    12721 gcatacgaga tacagcctga aagcatcagc gccagtggga ataaaccggc agccttaaaa
    12781 aatcccttaa aattcaactt catgattaat cctttaaacg agtagctaag ccgttgaaag
    12841 catactcttc tttccctcct gtttagcgcc tgatatccct tttcagattt ctgcccgacg
    12901 catgtcattt ttttatgcat tgttcttttt gtgatctaaa ttgtagtaca acaatataag
    12961 tttgtactac attacgcaca cggcaaacgc gaacgtcatc acgctggtac tacaaagttg
    13021 ccgcgttatg catcgatcgg ggtaaagtag agaagaacat acagagcaca aggactctcc
    13081 atgactctca ataaaaccga tcgcattgtc attacgctgg gtaaacagat cgttcacggc
    13141 aaatacgtac ctggctcgcc acttccggct gaggcggagc tctgtgaaga gtttgcaacc
    13201 tcgcgcaaca tcatccgtga ggtgttccgt tcgctgatgg cgaagcggct gattgaaatg
    13261 aaacgttatc gcggggcgtt tgtggcaccg cgtaaccagt ggaattacct cgacactgac
    13321 gtactgcaat gggtgctgga aaacgactac gacccacggc ttatcagtgc catgagcgaa
    13381 gtgcgaaatc tggtggaacc ggcgattgcc cgttgggcag cagagcgcgc gacttccagc
    13441 gatctggcgc agattgaatc ggcgctgaac gagatgattg ccaacaatca ggaccgcgaa
    13501 gcgtttaacg aagcggatat tcgctaccac gaggcggtgc tgcagtcggt acataacccg
    13561 gtgttacagc aacttagcat tgcgatcagt tcgttgcagc gggcggtttt tgaacgaacc
    13621 tggatgggcg atgaggccaa catgccgcaa acgctccagg aacataaggc gctgttcgat
    13681 gcaatacggc atcaggacgg cgatgcggca gagcaggcgg cgcttaccat gatcgccagc
    13741 tcgacacgaa ggttaaagga aatcacatga cagctcgcta catcgcaatt gactggggat
    13801 cgaccaatct gcgcgcctgg ctttatcagg gcgaccactg cctggagagc aggcaatcag
    13861 aagcaggcgt cacgcgcctg aacggaaaat ctccggctgc ggtgttagca gaagtcacga
    13921 ccgactggcg tgaagaaaat acgccagtgg tgatggcagg aatggtcggc agcaatgtcg
    13981 gctggaaagt tgctccgtat ttatctgttc ctgcccgttt ttcgtctatt ggcgaacaat
    14041 taacgtctgt tggcgacaat atctggatta ttcccggatt atgcgtctct catgacgata
    14101 accacaatgt gatgcgcggc gaagaaacac aattgatcgg cgcgcgagct ctggctcctt
    14161 cctctcttta tgttatgccc ggtacgcatt gcaaatgggt gcaggccgat agccagcaaa
    14221 tcaacgattt tcgcaccgtg atgaccggtg aattacatca tttactgtta aatcactcat
    14281 tgattggcgc aggtttgccg ccgcaggaaa actctgccga tgccttcgcg gctggccttg
    14341 agcgcggcct taatgcgccc gccatattgc cgcagctttt tgaagttcgc gcctcgcatg
    14401 tgctgggaac acttccccgc gaacaggtca gcgaatttct ctctggtttg ttgattggcg
    14461 cagaggttgc cagtatgcgc gactatgtga cccatcaaca cgccatcacc cttgtcgccg
    14521 gaacatcgct gaccgcgcgc taccagcaag cctttcaggc gatgggttgc gacgtgacgg
    14581 cggtggcggg cgacacggca tttcaggctg gtataaggag catcgctcat gcagtggcaa
    14641 actaaacttc cgctgatcgc cattttgcgc ggcattaagc ccgacgaggc gctggcgcat
    14701 gttggcgcgg tgattgacgc cgggttcgac gcggttgaaa tcccgctgaa ttccccacaa
    14761 tgggagcaaa gcattcccgc catcgttgat gcgtatggcg acaaggcgtt gattggcgca
    14821 ggtacggtac tgaaacctga acaggtcgat gcgctcgcca ggatgggctg tcagctcatc
    14881 gttacgccca atatccatag tgaagtaatc cgccgtgcgg tgggctacgg catgaccgtc
    14941 tgccccggct gcgcgacggc gaccgaagcc tttaccgcgc tcgaagcggg cgcgcaggcg
    15001 ctgaaaatat ttccgtcatc ggcttttggt ccgcaataca tcaaagcgtt aaaagcggta
    15061 ttgccatcgg acatcgcagt ctttgccgtt ggcggcgtga cgccagaaaa cctggcgcag
    15121 tggatagacg caggttgtgc tggggcgggc ttaggcagcg atctctatcg cgccgggcag
    15181 tccgtagaac gcaccgcgca gcaggcagca gcatttgtta aggcgtatcg agaggcagtg
    15241 caatgaaaat caccaaaatt actacgtatc gtttacctcc ccgctggatg ttcctgaaaa
    15301 ttgaaaccga tgaaggcgtg gtcggttggg gcgagcccgt gattgaaggc cgcgcccgta
    15361 cggtggaagc ggcagttcac gagctgggtg actatttgat tggtcaggat ccttcgcgca
    15421 tcaatgactt atggcaagtg atgtatcgcg ccggatttta tcgtggcggt ccaatcctga
    15481 tgagcgccat tgccgggatc gaccaggcgt tatgggatat caaaggcaaa gtgctgaatg
    15541 cgccggtctg gcaactgatg ggcggcctgg ttcgcgacaa aattaaagcc tacagttggg
    15601 tcggcggcga tcgtccggcg gatgttatcg acggcattaa aaccctgcgc gaaatcggct
    15661 tcgatacctt caaactgaac ggttgtgaag aactggggct aattgataac tcccgcgcgg
    15721 tagatgcggc agtcaacacc gtggcacaaa ttcgtgaagc ttttggcaat cagattgagt
    15781 ttggtcttga tttccacggt cgcgtcagcg cgccaatggc gaaagtgctg attaaagaac
    15841 tggagccgta tcgcccgctg tttattgaag agccggtgct ggcggaacaa gccgaatact
    15901 acccgaaact ggcggcacaa acgcatattc cactggcggc gggtgagcgc atgttctcac
    15961 gtttcgattt taaacgcgtg ctggaggcag gcggtatttc gattctgcaa ccggatctct
    16021 cccatgcagg cggtattacc gaatgctaca aaattgctgg aatggcagaa gcctatgatg
    16081 tgacccttgc gccgcactgt ccgctcggac cgattgcact ggcggcttgc ctgcatatcg
    16141 actttgtttc ctataacgcg gtacttcagg aacaaagtat gggcattcat tacaacaaag
    16201 gcgcggagtt actcgacttt gtgaaaaaca aagaagactt cagtatggtt ggcggcttct
    16261 ttaaaccgtt aacgaaaccg ggcttaggtg tggaaatcga cgaagctaaa gttattgagt
    16321 tcagtaaaaa tgccccggac tggcgtaatc cgctctggcg tcatgaagat aacagcgtag
    16381 cagagtggta attcctgcca cgtaagcccc tcatcgggca ctaaaacagc aatacaaaaa
    16441 tataaccctc tgtaaattac agggcatggt gagcggcttc gctatgccca aaatctggag
    16501 aaagctcgct atggatattc ccgttaatgc agcaaagccg gggcgtcggc gttatctgac
    16561 gctggtgatg atctttatta cggtggtcat ttgttatgtt gaccgcgcta acctggccgt
    16621 ggcttccgcc catattcagg aagagttcgg cattacgaaa gcggaaatgg gctatgtatt
    16681 ttcggccttc gcctggcttt atacgctatg ccagatcccc ggcggttggt ttttagatcg
    16741 cgtaggttct cgcgtgactt attttattgc gatatttggt tggtcagtgg cgactttatt
    16801 ccagggcttt gccacgggat taatgtcatt aattggtctg cgtgcgataa ccggtatttt
    16861 cgaagcgccc gctttcccga ccaataaccg gatggtgacc agctggttcc cggaacatga
    16921 acgcgcttcc gccgttggtt tttatacgtc tggtcagttt gtcggtctgg cgtttttgac
    16981 tccgctgctg atctggattc aggagatgtt gagctggcac tgggtgttca ttgtcactgg
    17041 tggtatcggc attatctggt cgctgatttg gtttaaggtt tatcagccgc cgcgcctgac
    17101 caaaggtatc agcaaagctg aactggatta cattcgtgat ggcggcggtc tggtggatgg
    17161 tgatgcgccg gtgaagaaag aggcgcgtca gccgttaaca gccaaagact ggaaactggt
    17221 gttccatcgt aaactgatcg gcgtttatct tgggcaattt gcggtggctt ctacactgtg
    17281 gtttttctta acctggttcc cgaactattt aacccaggaa aaaggaatca cggcgctgaa
    17341 agcaggcttt atgaccacgg tgccattcct cgcggcgttt gtcggcgtcc tgctctctgg
    17401 ctgggtcgcg gatctgctgg tacgtaaggg cttttcactg ggctttgcgc gtaaaacgcc
    17461 gattatctgc ggcttgctga tctccacctg cattatgggc gctaactaca ccaacgatcc
    17521 gatgatgatt atgtgcctga tggcgctggc attcttcggc aacggttttg cttcgattac
    17581 ctggtcgctg gtctcttctc tggcaccgat gcgcctgatt ggtttaaccg gcggcgtgtt
    17641 taacttcgcc ggtggtctgg gcggcatcac cgttccgctg gtggtggggt acctggcgca
    17701 gggttacggt ttcgcacctg cactggttta tatctccgcc gtcgcgttga ttggcgcgct
    17761 ctcttacatc ctgctggtgg gcgatgtgaa gcgcgttggc taatctttca actgtggaat
    17821 atgcgccata ccactgcgca taatccactt tcttaaagcc ggtgcggtca ggcatcggct
    17881 ttttactatc ttgccgaaga gttttaaacg cagtttgcgg gtggcgcgcc agtaagcggt
    17941 attgagcttc tctggctgct tcagtaacac cgaacgcaga atgtctgcgc tatccagcgc
    18001 atagctaatc ccctccagcg aactggcgct gataaatcct gccgcttcgc caatcagaaa
    18061 ggcgttgtcc ttaccgcaga caaaatcctg ccagcgtgag ggaaacagca ccgtgcattt
    18121 ttcgcttttc accgccttac caaactggaa ctgaaaggcg ctcattttct ctttcagcgt
    18181 cgtgaaacgc gtctgaccgt ctttcattgg ataagcaccg ccaaagataa aataaccgtc
    18241 tttgctgata ctccatgaat aacagttagt tatcgaatta tcaaagatgc aggagtagaa
    18301 cggcaccgga tgtttctctg cgaaccactg ctggatagcg acatatttac ggatttgatg
    18361 atccgggtag agatgtcgcc gcaccatcga gtttgcgcca tcggcaccga ccagatagcg
    18421 ggcagtaata tgttgctccc agccgtctgc acgaaaaatg acatgccatt tatcatcctc
    18481 acgccagatt tttcggcaca ggctatcgtg ataaacctca acgctggcgg ggatcagcga
    18541 tttcatccac aagtcgaaag catggcgatt aatattgata tagcttcgct ggtagttacg
    18601 cgtgagcgat gcggcgacgt cgacagtttt gacgctgaaa atctgcgggt tggcgatcac
    18661 atcgacagga agcgtcagtc catcgcgaat aaaagatcgc tgcgcgtccg gtgccagcag
    18721 accgccgcaa ggtttgctga aaccttcagt accacactgg tgctttttat ccagcgcgat
    18781 cacctgcatt ttgcctgcta attttcgcgc caacgccgat cctgccgggc cgaggccaat
    18841 aatcgccacg tcgaaatgtt ccatcttttc actcacattc atcacgatgg caaaagacta
    18901 ttgtctgaaa gtgaagtcct tgtgaagtat gatggcggga accgttttgt atcaggaccg
    18961 cgccatgaaa cagataacct ttgctccccg taatcacctg ctcaccaata ccaatacctg
    19021 gacgcccgac agccagtggc tggtatttga cgtgcgtcct tctggggcgt cgtttaccgg
    19081 cgagaccatt gagcgtgtga atatccatac aggcgaggtc gaggttatct atcgcgcgtc
    19141 acagggcgca cacgtcggcg tggtgaccgt tcatccgaag tcagagaaat atgtctttat
    19201 tcacggccct gaaaatcctg atgaaacatg gcattacgac ttccatcatc gacgcggagt
    19261 cattgctgaa ggcggcaagg tgagcaatct cgatgcaatg gatattaccg cgccgtatac
    19321 gccaggagcg ctgcgcggcg gcagccatgt gcatgtcttt agcccgaacg gtgaaagggt
    19381 gagctttacc tataacgacc atgtaatgca tgaactcgat ccggcgctgg atttgcgaaa
    19441 cgtcggtgtt gctgcgccgt ttggcccggt caacgtacaa aagcagcatc cgcgtgaata
    19501 cagcggtagc cactggtgcg tgctggtgag taaaaccacg cccacgccgc agcctggcag
    19561 cgatgaaatc aatcgtgctt atgaagaagg atgggtagga aatcacgcgc tggcgtttat
    19621 tggcgacaca ctttcgccaa agggcgagaa agtgccggag ctgtttatcg ttgagttacc
    19681 gcaagatgaa gctggctgga aagcggcagg tgatgcgccg ttaagtggaa cggaaaccac
    19741 cctgcccgcg ccaccgcgcg gcgtcgtgca gcgacgttta acctttaccc accatcgggc
    19801 ttatccgggg ttagtcaacg tcccgcgcca ctgggtgcgc tgtaatccgc agggtacgca
    19861 aatcgcgttt ttaatgcgtg atgataacgg cattgtgcaa ctgtggctta tctcgccaca
    19921 gggcggcgag ccgcgccagt taacccataa caaaacggat attcagtctg catttaactg
    19981 gcatccgtca ggagaatggt tgggctttgt gctggataat cgaattgctt gtgcccatgc
    20041 gcaaagcggc gaggttgagt atttaaccga aaaccacgcc aatccacctt ctgcggacgc
    20101 cgtggtcttc tcgccggatg gtcaatggct ggcgtggatg gaaggtggcc agctgtggat
    20161 caccgaaact gatcgctaaa tcagttatca ggcattctgg ccggtggaat aaccgcattg
    20221 gtggcttgcg cgttggcttc gcttttttca acgcgtgagc gcaccgagct gtctttgcgg
    20281 aacacatccc acggcagtaa aagcgtgtcc atcacagcgg taaacggcat atcgagaatc
    20341 gccagcgact tagtgcccca gttggtttca tcgtcgccaa tcatggtggc gctggcgcgg
    20401 gtgccgggat acgttccttc tttccctccg gtatgggaca tcacgctgga gcatcctccg
    20461 agtaatgtca ttccgctgca tatcatgaac gctaacaaca catttcttat cataataaat
    20521 tcatctgttg atcgtgggtg ttggcctgat gagttatagc gatcccttgc tgaaaataac
    20581 atcatcatta cgtcgcactg tggcggctat cgcactttaa cgtttcgtgc tgccccctca
    20641 gtctatgcaa tagaccataa actgcaaaaa aaagtccgct gataaggctt gaaaagttca
    20701 tttccagacc catttttaca tcgtagccga tgaggacgcg cctgatgggt gttctggcta
    20761 cctgacctgt ccattgtgga aggtcttaca ttctcgctga tttcaggagc tattgattat
    20821 gcgtaacttt gatttatccc cgctttaccg ttctgctatt ggatttgacc gtttgtttaa
    20881 ccacttagaa aacaaccaga gccagagtaa tggcggctac cctccgtata acgttgaact
    20941 ggtagacgaa aaccattacc gcattgctat cgctgtggct ggttttgctg agagcgaact
    21001 ggaaattacc gcccaggata atctgctggt ggtgaaaggt gctcacgccg acgaacaaaa
    21061 agagcgcacc tatctgtacc agggcatcgc tgaacgcaac tttgaacgca aattccagtt
    21121 agctgagaac attcatgttc gtggtgctaa cctggtaaat ggtttgctgt atatcgatct
    21181 cgaacgcgtg attccggaag cgaaaaaacc gcgccgtatc gaaatcaact aattccctaa
    21241 ggccgcctgg cgcggcctga catctccatg ctcgccgtca gggagcatat gcgaatcttc
    21301 ggatttgcag gtacttactc gcttcttaga aggagaaatg actatgcgta acttcgattt
    21361 atccccactg atgcgtcaat ggatcggttt tgacaaactg gccaacgcac tgcaaaacgc
    21421 cggtgaaagc cagagctttc cgccgtacaa cattgagaaa agcgacgata accactaccg
    21481 cattaccctt gcgctggcag gtttccgtca ggaagattta gagattcaac tggaaggtac
    21541 gcgcctgagc gtaaaaggca cgccggagca gccaaaagaa gagaaaaaat ggctgcatca
    21601 agggcttatg aatcagccat ttagcctgag ctttacgctg gctgaaaata tggaagtctc
    21661 tggcgcaacc ttcgtaaacg gtttactgca tattgattta attcgtaatg agcctgaacc
    21721 catcgcagcg cagcgtatcg ctatcagcga acgtcccgcg ttaaatagct aactagctat
    21781 tctctttgcc ccgccattct ggtggggctt tttttgtctt acttactcaa caatgcgtta
    21841 aatttcgact gtttaagata tttcggcacg ttctttcccg tgataatgtg cgctataagg
    21901 caaatctgct tcaccgattc agccataatc cgtagcaatt aaaaatgtta tctcaagaga
    21961 aatggacgat gagtgatata gcattaacgg tcagtattct ggctttggtg gcagtcgtcg
    22021 gtttgtttat cggcaatgtc aaatttcgcg gcataggatt aggtattggc ggcgtgctgt
    22081 ttggtgggat catcgtcggc cattttgttt ctcaggcggg gatgacatta agtagcgata
    22141 tgctgcatgt tattcaggaa tttggcctga tcctgttcgt ttatactatc gggattcagg
    22201 tagggccggg cttctttgcc tcattgcgcg tctccggatt acgcctcaac ctgtttgctg
    22261 ttctgatcgt catcatcggt ggtctggtta ccgccatcct gcataaactg tttgatattc
    22321 cactgccggt agtgctgggg attttctccg gtgcggttac caatacgcca gcgctggggg
    22381 cagggcagca gattttgcgc gacttgggta caccaatgga aatggtcgat cagatgggga
    22441 tgagttacgc gatggcgtat ccattcggca tttgcgggat tttgttcacc atgtggatgt
    22501 tgcgggttat tttccgcgtc aatgtcgaga cagaagctca gcagcacgag tcttcacgca
    22561 ccaatggcgg cgcgctgatc aagactatca atattcgcgt tgagaaccct aacctgcatg
    22621 atttagccat taaagatgta ccgattctca acggcgacaa aattatctgc tcgcgtctga
    22681 aacgcgaaga aaccctaaaa gttccttcgc cagataccat tatccaactg ggcgatttgc
    22741 tgcatctggt ggggcagcca gcggatttac ataatgcgca actggtgatt ggtcaggagg
    22801 tcgatacttc gctgtccacg aaaggcactg atttgcgcgt cgagcgtgtg gtggtcacca
    22861 atgaaaacgt gctcggaaaa cgtattcgcg acctgcactt taaagaacgc tatgacgttg
    22921 ttatctcgcg cctgaaccgt gccggggtcg aactggtcgc cagtggcgat atcagcctgc
    22981 agttcggcga tatcctcaat ctggtggggc gtccgtccgc aattgatgcc gttgccaatg
    23041 tgctggggaa tgcgcagcaa aaactgcaac aggttcagat gctgccagtg tttattggca
    23101 tcgggcttgg cgtattgtta ggttctattc ccgtctttgt gcctgggttc ccggccgcgt
    23161 tgaaactggg gctggcgggc ggtccgctga ttatggcgtt gatcctcggg cgtatcggca
    23221 gtatcggcaa gctgtactgg tttatgccgc caagtgccaa cctcgcgctg cgggagctgg
    23281 ggatcgtgct gttcctctcg gtcgttggtc tgaaatctgg tggagatttt gtgaataccc
    23341 tggtcaatgg cgaagggcta agctggattg gttatggtgc cctgatcacc gccgttccgc
    23401 tgattactgt tggcattctg gcgcggatgt tagccaaaat gaattacctg accatgtgcg
    23461 ggatgctggc aggttccatg accgatcctc cggcgctggc gtttgctaat aatcttcatc
    23521 caaccagcgg tgcggcggcg ctctcttacg ccactgtcta tccgttagtg atgttcctgc
    23581 gcattatcac cccccaatta ctggcggtgc tcttctggag tatcggttaa ctcttttccg
    23641 ggtggagtcg ccgtaaatgg tactccacct ggtattcgct ggcatttctg aacatcactg
    23701 aataattcaa aaactccccg ctctcgctat atgacaacga ggtgatccgc agcagtggcg
    23761 tgtgttccgc taccttcatt tgccgcgcca gcagtctatc ggcgagcact ggcgtcaggc
    23821 tttcataatt accgccaatc aaaatcccgc actcttgttc aatatattca aacttcgacc
    23881 cttccagatg ttgcagcgaa agattacgga acagttttac cggcatatag ctgtcttcca
    23941 gcatcagcgg tttcccttcc acaaaacgaa cgcgacggga gaagtagatc tgctcgttga
    24001 tttgaatccg taactggctg gcaatggccg gaggcgcagg catgatttca aaaatcagca
    24061 cctggctggt gaccgttttt ccctgccgtt ttaacacctc caccagcccg gtcaggctgg
    24121 cggtttgatg cagtacatct ttgcgcacca ggtaagtgcc gctgccgtgg cggcggacca
    24181 ccaatcccca cgctaccagc aggtcaatgg ctttacggat ggtcatccgc gataccgcaa
    24241 attcttccgc caactttttt tcaccgggaa gaaggctatt tagcgtgaaa tctgcggaat
    24301 taggtcgaat tcttaaccgc tccgcaatgc ttttgtagat catctctaga tctctttttt
    24361 gcctgcaagc acgggtaatg cgcccacaag ttctgcttaa tcgattgaaa aaacatcttt
    24421 tttttaaaga tgtgttcgat gctgtgtacc tgtgctcaca gatgtctact ttttcgcgaa
    24481 aacgtagatc tctaccgccc aacgaaaagc atgaaagcga tcacgaatcc cattgggtcg
    24541 tcatgttctg ccgatcgcat cttcctatcc tcgctccagg cctgccgcat aaccaatcag
    24601 gcttcctact tacagaattg agaaaagagg atgtggaaat gctcagtcaa attcaacgct
    24661 ttggcggcgc gatgttcacg ccagtgctgc tgtttccctt cgccgggatt gtggtgggtc
    24721 ttgccatctt gctgcaaaac ccgatgtttg tcggggaatc actgaccgat ccgaacagtt
    24781 tattcgcgca aatcgtacac attattgaag agggcggttg gacggtgttc cgtaatatgc
    24841 cgctgatttt tgctgtcggt ttacccattg gccttgctaa gcaagcgcag gggcgtgctt
    24901 gtctggcggt gatggtgagt ttcctgacct ggaactattt catcaacgcg atgggaatga
    24961 cctggggaag ctacttcggc gtcgatttca ctcaggacgc ggtggcaggt agcggtctga
    25021 caatgatggc cgggattaaa accctcgata ccagcattat cggcgcaatt atcatttccg
    25081 gcattgtgac ggcgctgcat aaccgtctgt tcgataaaaa actgccggtt tttctcggca
    25141 ttttccaggg gacgtcttat gtggtgatta tcgccttcct ggtgatgatc ccctgtgcct
    25201 ggctgacgtt gctcggctgg ccaaaagtac aaatggggat tgaatctctg caagcgttcc
    25261 tgcgttcggc gggtgcgctt ggggtgtggg tttacacctt cctcgaacgt attctgatcc
    25321 caaccggttt acaccacttc atctacggac cgtttatctt tggtccggca gctgttgaag
    25381 gcggcattca gatgtactgg gcgcagcatc tgcaagagtt cagtctgagc gccgagccgc
    25441 tgaaatcgtt gttcccggaa ggcggttttg ccctgcacgg taactcaaaa atctttggtg
    25501 ccgtgggcat ttctttagcg atgtacttca ctgccgcacc ggaaaatcgg gtaaaagtgg
    25561 cgggcttgct gattcccgca accttaaccg ccatgctggt gggaattacc gaaccgctgg
    25621 aatttacctt cctgttcatt tcaccgttgc tgtttgcggt acacgctgtg cttgcggcct
    25681 caatgtcgac cgtgatgtat ctctttggtg tggtgggcaa catgggcgga ggtctgattg
    25741 accaggtttt accgcaaaac tggatcccga tgttcagcaa ccacgcggat atgatgctga
    25801 cccaaatcgc cattgggttg tgctttaccc tgctgtactt cgtggttttc cgcacactga
    25861 ttctgcagtt caacatgtgc acgccgggac gtgaagatgc ggaagtgaaa ctctactcaa
    25921 aagccgaata caaagcctcg cgaggtcaaa ccaccgcggc agagccaaaa aaagagctgg
    25981 atcaggctgc cggtatcctg caagccctgg gcggggtcgg caatatctcc agcattaaca
    26041 attgtgcgac gcgtctacgc attgcactgc atgacatgtc acaaacgctg gatgacgaag
    26101 tctttaaaaa gctgggagcg cacggcgtct tccgtagtgg cgatgccatt caggtgatca
    26161 ttggtctgca tgtatcccag ctgcgtgaac agctcgatag cttaattaat tctcatcaat
    26221 cagcagaaaa tgttgccatt acggaggcag tataatgacc aaattctcag tggttgtcgc
    26281 aggcggtgga agcaccttta cgccaggcat cgtgttgatg ctcctggcga atcaggagcg
    26341 tttcccgctt cgtgcactga aattttatga taacgatggc gcgcggcagg aagtgattgc
    26401 cgaagcctgt aaagtcatcc ttaaagagaa agcgccggac attgcgttta gttacaccac
    26461 cgatcctgaa gtggcgttca gcgacgttga ttttgtcatg gcgcacatcc gcgtcggcaa
    26521 atacccgatg cgcgaactgg atgaaaaaat cccactgcgc cacggtgttg ttggtcagga
    26581 aacttgcgga cccggcggaa tagcgtacgg catgcgttcc attggcggcg tcctggaact
    26641 ggtggattat atggaaaaat attcaccaaa cgcctggatg ctcaactact ccaacccggc
    26701 agcaattgtc gcagaagcca cgcgtcgtct gcgcccgaat gcgaaaatcc tcaacatctg
    26761 tgacatgcca atcggtattg aaagccggat ggcgcaaatt gttgggctgc aagatcgcaa
    26821 acagatgcgc gtgcgctact acggcctgaa ccactggtgg tcggcgatat cccgcagttt
    26881 cagaaagggt taatgagtca gcaagtggcg gtggaaaaac tggtcgtgga cgcctgggaa
    26941 cagcgttcat atcagcacct gtggtaggcg attacgttgt cgaaaacggt accgagcgcc
    27001 tcggtcgcca aagctattct ggatgagttg ctggaggcca acaaagcgta ctggccagag
    27061 ttacgttaat tgtgtggact ggcatccgtt gggtgctggt ccgtattctt tagcacggct
    27121 gacggcgcat aaccaaattt atgtttaaag gctttgctga aatgaaagga gtcgaagaag
    27181 ttgagcgtat cagcgacttc gccaaccgag tttccttgtt gctttaatag cgatagcgcc
    27241 agatccagac gcgcatcgag gtaatattct ttcggcgtct tgccggtata gcgtaaaaac
    27301 agacgacgca accaggcttc gctacagggg atcgtggcag ccatatcagc tacgctccag
    27361 cgttgttgca gactggcatg taaagtggca attaattttt caatttgccg ccgttgtgga
    27421 tcttttttac catctgcgta cataagacaa atccactgat aaataatttt cgtcagaaaa
    27481 gcgactgcca gattattttt tattgcttct ggtgaagtta ttaactcagc aacttcggtg
    27541 agttcctgat tataaatttc gccattataa ataacgcttt gctgaccaac gggaatatcc
    27601 atcatactgg tgggggtaaa ttccatccag tactgttccc agactaaacc ttcacagtga
    27661 taagagtgaa tatccattgg ctttaagaat ataatacagt tgccatgcag tgttatttgt
    27721 tcgccattct ttaaaagaat tttcccacat ccttgtacgg tatacaccgc aacccatgag
    27781 ttggcgataa gcggtttctt cttgtcccgc ggccagatca cccggtaatg ctcgtcggcg
    27841 taggtatgct agagcgagat cagagaaatt ccgctggaga tcacattttc gttaatcaat
    27901 aagggaatat tgtacggagt ttcgtttttt acatctgaac tttgcaattt tccattcatt
    27961 ttaatatccc tccggcgaat tatgctcaca acctgaactt aacagaaaac aatccgttca
    28021 gggatacgac gtttgtattt taagaatctg actgcctgac ccgacgcatt ttacctctcc
    28081 ctatattcat gcgtccggga cataaacata aataagggct atgagatgaa ttcgttacaa
    28141 atcttgagtt ttgtcggttt tacgctgctg gtggcggtga tcacctggtg gaaggtccgc
    28201 aaaacagata ccggatcgca acaaggctat tttcttgccg gacgttcact aaaagcgccg
    28261 gttattgccg cttcgttaat gctaaccaac ctttccacgg aacaactggt cggcctttcc
    28321 gggcaggcct acaaaagcgg catgtcggtg atgggctggg aagtgacttc agcggtgacg
    28381 ctgatcttcc tcgcgctaat ctttttaccg cgctatctga agcgcggcat tgccaccatc
    28441 cccgattttc tggaggaacg ttatgataaa acgacgcgta ttatcatcga cttctgcttc
    28501 ctcattgcca ccggcgtctg ctttctgccg attgttctct actccggcgc gttggcgctc
    28561 aacagcctgt ttcacgtcgg ggaatcgcta cagatttctc acggtgcggc tatctggcta
    28621 ctggtaattt tgcttggtct ggcgggaatt ttgtatgcgg tgatcggcgg actgcgcgca
    28681 atggcagtgg cggactccat caacggtatt gggctggtta tcggcgggtt gatggtgccg
    28741 gtatttggcc tgatcgcgat gggcaagggc agctttatgc agggcattga gcaactcacc
    28801 accgttcacg ccgagaaatt aaactcaatc ggtggcccga ccgatccctt gccgattggc
    28861 gcggcattta ccggtttgat tctggtgaac accttttact ggtgtacaaa tcagggcatc
    28921 gtgcaacgca cgctggcgtc aaaaagcctg gcggaagggc aaaagggggc gctgttaacg
    28981 gcggtgctga aaatgctcga cccgctggta ctggtgctgc cagggttgat tgcgtttcat
    29041 ctgtatcagg atttaccgaa agccgacatg gcctacccga cgctggtcaa taacgttctg
    29101 ccagtgccaa tggtgggttt cttcggcgcg gtgttatttg gtgcggtgat cagtaccttc
    29161 aacggctttc tgaatagcgc cagtacgtta ttcagtatag gtatttaccg tcgcatcatt
    29221 aaccagaatg ccgagccgca gcagctggtc accgtcgggc gcaaatttgg tttctttatc
    29281 gctatcgttt cggtgctggt cgcgccgtgg atcgccaacg cgccgcaggg gctgtatagc
    29341 tggatgaaac agctcaacgg catttacaac gtgccgctgg ttaccatcat cattatgggc
    29401 tttttcttcc cgcgcatccc ggcgctggcg gcaaaagtgg cgatggggat tggcataatc
    29461 agctacatca ccatcaacta tctggtgaag ttcgacttcc atttcctcta tgtgctggcc
    29521 tgtacgttct gcatcaacgt ggtcgtgatg ctggtgatcg gttttatcaa accgcgcgcc
    29581 acgccgttca ccttcaaaga tgcgtttgcg gtagacatga aaccgtggag aaacgtcaag
    29641 atcgcgtcaa ttggcatcct gttcgcgatg attggcgtct atgccgggct ggctgaattc
    29701 ggcggctacg gtacgcgctg gttagcgatg atcagttatt tcattgccgc cgtagtgatt
    29761 gtctacctga tttttgacag ctggcggcat cgtcacgacc cagccgtaac ctttactccc
    29821 gacgcgaagg atagcctatg aaacgcccca attttctgtt catcatgacc gatacccagg
    29881 ccaccaatat ggtcggttgc tatagcggta aaccgctgaa tacgcaaaat attgatagtc
    29941 tggcggcgga aggtattcgc tttaattccg cctacacctg ttcaccggtt tgtacgcctg
    30001 cacgcgccgg actatttacc ggtatctacg ctaaccagtc cggcccgtgg accaacaacg
    30061 tcgcgccagg caaaaacatc tccactatgg ggcgctactt taaggatgcc ggctatcaca
    30121 cctgttacat cggcaaatgg catctcgacg gtcatgacta tttcggcact ggcgagtgtc
    30181 cgccggagtg ggacgctgat tactggttcg atggggcgaa ctaccttagc gaactgacgg
    30241 aaaaagagat cagcctgtgg cgcaatggcc taaacagcgt tgaggattta caggcgaacc
    30301 atatcgacga aaccttcacc tgggcgcacc gcatcagcaa tcgggcggta gattttctgc
    30361 aacagcccgc gcgcgccgag gaacccttcc tgatggtggt ttcgtatgat gagccgcatc
    30421 acccgttcac ctgtccggta gagtatttag agaaatacgc tgatttttac tacgatctgg
    30481 gcgagaaagc tcaggatgac ctggcgaaca aaccggaaca tcaccgctta tgggcgcagg
    30541 cgatgccatc gccagtcggt gatgacgggc tttatcacca tccgctctat tttgcctgta
    30601 atgactttgt tgatgaccaa atcggacggg tcatcaatgc cttaacgcca gagcaacgtg
    30661 aaaatacgtg ggtcatttat acttccgatc acggcgaaat gatgggcgca cataagctga
    30721 tcagtaaagg agcggcgatg tatgacgaca tcacccgtat tccgctgatc atccgttcgc
    30781 cgcaagggga gcggcgacag gtcgatacgc cagtcagtca tatcgattta ctgccgacaa
    30841 tgatggcgct ggcagatatt gaaaaaccag agattctgcc gggggaaaat atccttgccg
    30901 tgaaagagcc acgcggtgta atggtggaat ttaaccgcta cgagattgag catgacagct
    30961 ttggcggttt tattccggtg cgttgctggg tgacggatga ctttaaactg gtactcaacc
    31021 tcttcaccag tgatgaactt tacgatcgcc gtaatgaccc aaatgaaatg cataacctga
    31081 tcgatgatat ccgttttgca gacgttcgca gcaaaatgca tgacgcctta ttggattaca
    31141 tggacaaaat tcgcgatccg ttccgcagtt accaatggag cctgcgtccg tggcgtaaag
    31201 atgcactgcc gcgctggatg ggggcatttc gtccacgccc acaagatggc tattcgccgg
    31261 tggtacgtga ctatgacacc ggcctaccga cgcaaggagt gaaagtggaa gagaaaaaac
    31321 agaagttctg acgataatgc ccgctgatga acacccggcg ggcattattc aggctgctta
    31381 aaccagtgaa ccgccggtca ggaaggaaaa aatcaggaat atcagcaaaa tgctgcacac
    31441 gatgccgata ccaaaaagca aggtatggaa agccagcgtc ccgccgccaa aaaccagcgc
    31501 gccgcgaatg ccccagcgat tttccttacg cataccgcca ataaatgcgc aaagtagcgc
    31561 agcaatggcc agtgcgatac cgctttgatc gaggatttta tcgacatcta cgtcctcttt
    31621 tttctccacg gtggtaattt tttcgccttt tattccggcg agaagacctc ttttaatttc
    31681 agccgttttg tccgccacga tgctttcaaa cgttggcggt ggtgcggaaa aagggccgaa
    31741 tgaaaagtgg ataatcccca tcattaaagc gatggcaccg aacagcatgc ccaatgaact
    31801 gattttattt tgagctatga tacccatctg acctccttgt agcggatggc gaaagcatat
    31861 caggaaacgg gcggacgaaa ttcttaaaaa aaaaccgcta aaggtagagc gttagtgaaa
    31921 ttgcaaagga gcaacaatga aaatttcccg ccttggagaa gcgcccgatt accgcttctc
    31981 gctggctaat gagcgtactt ttctggcgtg gatccgtacc gcactaggat ttctggcggc
    32041 gggggttggg cttgatcaac ttgcgccaga tttcgccacg cccgtcattc gtgaactgct
    32101 ggcgttgttg ttgtgtctgt tttccggcgg gttagcgatg tatggctacc tgcgctggtt
    32161 acgcaatgaa aaagcgatgc gtctgaaaga ggacctgccc tacaccaaca gcttattaat
    32221 cattagctta atattgatgg tcgtggcggt gatcgttatg ggactggtgt tgtatgccgg
    32281 atagccgcaa agccagacgc attgccgacc caggactgca gccggagcgc acatcactgg
    32341 cgtggtttcg taccatgctg ggctacggcg cgttgatggc gttggctatc aaacacaact
    32401 ggcaccaggc gggcatgtta ttctggattt cgattggcat tctcgccatc gtggcgctga
    32461 tcctctggca ctacacccgt aatcgcaatt taatggatgt cacgaatagc gatttttccc
    32521 aatttcacgt agtccgtgac aaatttttga tctccctcgc ggtgttatct ctcgcaatac
    32581 tgtttgctgt aacgcatata catcaactta tcgtatttat tgagagagtc gcatgacagg
    32641 aagtcaggtc attgatgctg aagaggacag gcataaactg gttgtagagt acaaggatgc
    32701 tctgcaacct gccgattttt atcacaattt caaacagcgg ggcatccgct ccgtacagct
    32761 cattccctat cttgaatttg atgaccgggg cgatctgacg gccgcttcgg tgacagcaga
    32821 gctgtggggg aaatttttaa ttgccctgtt cgagtgttgg gtgcgggcag atatcagtcg
    32881 tatctcgatt gagctttttg acgccacctt acaaaaatgg tgtggcagcg aaaacccgca
    32941 accgcgccgt gactgccagg cgtgcgactg gcatcgcctg tgcccgcacg ctcgccagga
    33001 gacgccagac agcgtgcttt gtgcgggcta tcaggcgttt tattcttact ccgcaccgca
    33061 tatgcgggtg atgcgtgatc tgataaagca gcatcgttct cccatggagc tgatgacgat
    33121 gctgcggtga cgtgcgctta aacgggctgc ccctgatgcg acatccgcgt cgccagcggc
    33181 agccagcaca gcacgatcaa caatcccatt aaggtcatca acaaccccag gctgccctga
    33241 ccggtttgcg gcaacatcgc agagagcgac gccagcacgc cggaaccaat gttttgcaga
    33301 ccgccgacca gcgcgccagc cgtgcccgcc aggaagggga acggctccat cgcgccgctg
    33361 gtcgccagcg gaaacagcat cccggcaccg aaaaagaaca gcgcggcggg aacgagcagc
    33421 gtccagacat tcatcacgcc aaaccagtcg gggatccaca tcagcaagcc agccagcagg
    33481 cagcagataa ccgactgcca cattaacgtg gagaagcgtt tattgggacg tccggcaaac
    33541 catgcgccaa aaaacgctgc cggaatcggc agaataaaca aaatactgac cgtcatactg
    33601 ctcagcccta acaccgcgcc catcagcacg ccggagcagg cttcaaaggc ggcaatcccg
    33661 gccagaccgc caatcagcat cagcaaataa cagttaaaac cgctgttacc gaaaagcgtt
    33721 ttataactgg taagcaggcg cgtgcgcggt gcatcgaccg gacgcgtttc cggcatccag
    33781 cgggccatac tgaaggtcac accagcacaa agaaccaaca aaaagagata acaggcgcgc
    33841 cagttccaca tcgtatccag cagaccgccg attagcggtg cgagcaacgg actgacgaga
    33901 atccccatgt ttaacaggct gttagcatgg cgcaactgtg tccgttcata taaatctcgc
    33961 ggcaaagtac gcgccattac gccgccaacg ccggtgccca tcccctgcat cgcgctggcg
    34021 gcaatcaaca ccgtcaaact ggaggtcgtg accgcgacca gcgttgccag cataaaaatg
    34081 gacattccga cgaggatcac cggtcggcgg cccacgcggt cggaaatcgg gccataaaac
    34141 agctgtgaga caccgtaagt cagcagataa gcgcccatta cgctctgcac cgccccttca
    34201 cggacgttga gatcgcgcgc catatcggca atagctggaa tataaatggt ttgcgccatc
    34261 tgaccgacgg ccacgagtaa taccaacatc aataacaaat tgacgtttct ttgccttttc
    34321 attatcacgg atgcttttat aaaaataatt agaataagtc actacgcatt cccataaatg
    34381 cgggagagga atctatcaca ttgatgaatg ttagccagat atacgccagg aatggggaat
    34441 tgtttagcgg cagaatatgt aaacaaaagc ggcaataaat gttgccggga gggattacgt
    34501 gcgaactgta accctctccc caaaggggcg agggggaaac caccgttccc tttcagaaaa
    34561 tgagctaaac attgcacggg acggtcccct cgcccctttg gggagagggc tagggtgagg
    34621 ggcacaggca gcgcaggtgc aaacattctg ctggtagaag aagcccccaa ctccaacccg
    34681 cacgctaaat ataaaagggg agcggttacc cgctcccctt tggtgcgact tgaatctgaa
    34741 ttacttcagg tatttcagaa cagcatcaag cagttgcagt gctgcaacaa tgagtttgag
    34801 gataagaatg gcgatatcca ccaggctcat acgcgtctcc tgtggttcag gagcactgcc
    34861 agctgacgta cctttccgct gcctgttgcc agctgttgtg cacgcggtat cttaaggaga
    34921 aacgttgtgc ttgttgacat aacacagtgt gctcacgcgg ggttaaggcg ctgacggcac
    34981 cacccgtttc agccaggact tagtgcgccg ggtaacgatg cggcccccgc ataacacatt
    35041 gcgtacagtg atattataga agtttactgt ataaataaac agtaatattt ggacaaaacg
    35101 caaactgtga tcgatactca actatgccct aaatacgtca aaattcgtgc cgaaattgcg
    35161 cgttctgcgc ggaacacgta tactttcagt gttgacataa tacagtgtgc tttgcggtta
    35221 ccagccgcag gcggctgatg aaacctcgct ccggcggggt tttttgttac ctgcaattca
    35281 gtacaaaacg tgatcagccc ctcaattttc ccttgctgaa aaattttcca ttgtctcccc
    35341 tgtaaagctg tgcttgtata aatattgtta aacacaaaac caacaaggtc cccaatgact
    35401 acttccatgt tcaacgcaaa actactacca actgcgctat ccgccgcagt ggtcgtcgtg
    35461 cgtgtggtgg tggtcgtcgg caatgcgccg tagggactgg aacaacacac gattccaaaa
    35521 ccccgccggc gcaaaccggg cggggttttt cgtttaagca cctcccggaa agtcggccca
    35581 gaagaaaagg actggagcat ggcaagttcg ggcacaacat cgacgcgtaa gcgctttacc
    35641 ggcgcagaat ttatcgttca tttcctggaa cagcagggca ttaagattgt gacgggcatt
    35701 ccgggcggtt ctatcctgcc tgtttacgat gccttaagcc aaagcacgca aatccgccat
    35761 attctggccc gtcatgaaca gggcgcggga tttatcgctc aggggatggc gcgcaccgac
    35821 ggtaaaccgg cggtctgtat ggcctgtagc ggaccgggtg cgactaacct ggtgaccgcc
    35881 attgccgatg cgcggctgga ctccatcccg ctgatttgca tcaccggtca ggttcccgcc
    35941 tcgatgatcg gcaccgacgc cttccaggaa gtggacacct acggcatctc tatccccatc
    36001 accaaacaca actatctggt cagacatatc gaagaactcc cgcaggtcat gagcgatgcc
    36061 ttccgcattg cgcaatcagg ccgcccaggc ccggtgtgga tagacattcc taaggatgtg
    36121 caaacggcag tttttgagat tgaaacacag cccgctatgg cagaaaaagc cgccgccccc
    36181 gcctttagcg aagaaagcat tcgtgacgca gcggcgatga ttaacgctgc caaacgcccg
    36241 gtgctttatc tgggcggcgg tgtgatcaat gcgcccgcac gggtgcgtga actggcggag
    36301 aaagcgcaac tgcctaccac catgacttta atggcgctgg gcatgttgcc aaaagcgcat
    36361 ccgttgtcgc tgggtatgct ggggatgcac ggcgtgcgca gcaccaacta tattttgcag
    36421 gaggcggatt tgttgatagt gctcggtgcg cgttttgatg accgggcgat tggcaaaacc
    36481 gagcagttct gtccgaatgc caaaattatt catgtcgata tcgatcgcgc agagctgggt
    36541 aaaatcaagc agccgcatgt ggcgattcag gcggatgttg atgacgtgct ggcgcaattg
    36601 atcccgcagg tggaagcgca accgcgtgca gagtggcacc agttggtagc ggatttgcag
    36661 cgcgaattcc cgtgtccaat cccgaaagcg tgcgatccat taagccatta cggcctgatc
    36721 aacgccgttg ccgcctgtgt cgatgacaat gcgattatca ccaccgatgt gggtcagcat
    36781 cagatgtgga ccgcgcaagc ttatccgctc aatcgcccac gccagtggct gacctccggt
    36841 gggctgggca cgatgggttt tggcctgcct gcggcgattg gcgcggcgct ggcgaacccg
    36901 gatcgcaaag tgttgtgttt ctccggcgac ggcagcctga tgatgaatat tcaggagatg
    36961 gcgaccgcca gtgaaaatca gctggatgtc aaaatcattc tgatgaacaa cgaagcgctg
    37021 gggctggtgc atcagcaaca gagtctgttc tacgagcaag gcgtttttgc cgccacctat
    37081 ccgggaaaaa tcaactttat gcagattgcc gccggattcg gcctcgaaac ctgtgatttg
    37141 aataacgaag ccgatccgca ggctgcattg caggaaatca tcaatcgccc tggcccggcg
    37201 ctgatccatg tgcgcattga tgccgaagaa aaagtttacc cgatggtgcc gccaggtgcg
    37261 gcgaatactg aaatggtggg ggaataagcc atgcaaaaca caactcatga caacgtgatt
    37321 ctggagctca ccgttcgcaa ccatccgggc gtaatgaccc acgtttgtgg cctttttgcc
    37381 cgccgcgctt ttaacgttga aggcattctt tgtctgccga ttcaggacag cgacaaaagc
    37441 catatctggc tactggtcaa tgacgaccag cgtctggagc agatgataag ccaaatcgat
    37501 aagctggaag atgtggtgaa agtgcagcgt aatcagtccg atccgactat gtttaacaag
    37561 attgcggtgt tttttcagta accgctcaag gcttgaacaa catcgcgctt atcgttaagg
    37621 taagcgcgtt tttttttacc cgccaggaca agaccatgat caccgttgcc cttatagacg
    37681 atcacctcat cgtccgctcc ggctttgcgc agctgctggg gctggaacct gatttgcagg
    37741 tagttgccga gtttggttcg gggcgcgagg cgctggcggg gctgccgggg cgcggtgtgc
    37801 aggtgtgtat ttgcgatatc tccatgcccg atatctccgg tctggaactg ctaagccagc
    37861 tgccgaaagg tatggcgaca ataatgctct ctgttcatga cagtccggcg ctggttgagc
    37921 aggcgcttaa cgcgggggca cgcggctttc tctccaaacg ctgtagccct gacgaactga
    37981 ttgcggcggt gcatacggtt gccacgggcg gctgttatct gacgccggat attgccatta
    38041 aactggcatc cggtcgtcag gacccgctaa ccaaacgcga acggcaggtg gcggaaaaac
    38101 tggcgcaagg aatggcggtg aaagagattg ctgccgaact gggcttgtcg ccgaaaacgg
    38161 tgcacgtcca tcgcgccaac ctgatggaaa aactgggtgt cagtaacgac gtagagctgg
    38221 cgcgccgcat gtttgatggc tggtgatgaa gacgttgttc tcccgcttaa ttaccgttat
    38281 tgcctgcttt tttatcttct ctgccgcatg gttttgcctg tggagtatca gcctgcatct
    38341 ggttgagcgc cctgatatgg cggtgctgtt atttccgttt ggtctgcgtc tggggctaat
    38401 gctgcaatgc ccgcgcggat actggcccgt attgctgggt gcggagtggc tgctgattta
    38461 ctggctaacg caggcggtcg gtttaaccca ttttccgtta ttgatgatcg gtagtttact
    38521 gacgttactg cccgtagcgc tgatctcgcg ctatcgccat cagcgtgact ggcgcacctt
    38581 gctgttacag ggggcggcgt taacggcggc ggcgttgttg cagtcgctgc cctggctttg
    38641 gcacggcaaa gagtcgtgga atgcgctgtt gctgacttta actggcggcc tgacgctggc
    38701 cccgatatgt ctggtgttct ggcactatct cgccaataac acctggctgc cgctcggccc
    38761 gtcactggtt tctcaaccga tcaactggcg cgggcggcat ctggtctggt acttgctgtt
    38821 gtttgttatt agtctctggc tccagttggg attgccggac gaactgtcgc gctttacgcc
    38881 attctgcctg gcgctgccga ttatcgcgct ggcctggcac tacggttggc aaggggcgct
    38941 gatcgcgacg ttgatgaacg ccatcgcgct gatcgccagt caaacctggc gcgatcatcc
    39001 ggtggattta ttgctctcgt tgctggtgca aagtctgaca gggttgttac tgggcgctgg
    39061 catccagcgg ttgcgtgaac ttaaccagtc gctgcaaaag gaactggcgc gcaatcagca
    39121 tctggctgaa cggttgctgg aaaccgaaga gagcgtgcgc cgtgatgtgg cgcgtgagct
    39181 gcatgatgat atcggtcaga ccatcactgc tattcgtact caggcgggca ttgttcagcg
    39241 gctggcggca gataacgcca gcgtgaagca gagcgggcag ctcatcgaac aactatcgct
    39301 gggcgtttac gacgcggtgc gccgtttgtt gggtcggtta cgtccgcgcc agttggatga
    39361 tctcaccctg gagcaggcca tccgctcact gatgcgggaa atggagctgg aagggcgcgg
    39421 tattgtcagc catctcgaat ggcgaatcga tgaatcagca ttaagtgaaa accagcgcgt
    39481 gacgctgttt cgtgtctgcc aggaagggct gaacaacatt gtgaaacatg ctgatgccag
    39541 cgcggtcacg ctgcaaggct ggcagcagga tgaacggttg atgctggtta ttgaagacga
    39601 tggcagcggt ttgccgccgg gttccgggca acaaggtttt ggcctcaccg gaatgcgcga
    39661 gcgcgtaacg gcgctgggtg gcacattaca catttcctgt ctgcacggca cgcgtgtcag
    39721 cgtttctcta cctcaacgct atgtctaagg tttgatgatg ttgccgtttc tgaaagcgcc
    39781 tgccgatgcg ccattaatga ctgataaata tgaaattgat gcccgctatc gctactggcg
    39841 tcggcatatt ctgctgacca tctggctggg ttatgcgctg ttttacttca cgcggaaaag
    39901 ttttaacgcc gccgtaccag aaatccttgc taacggcgtg ctcagccgta gcgatatcgg
    39961 cctgttagcg accctgtttt acattaccta tggcgtgtcg aagtttgtct ccggcattgt
    40021 cagcgatcgc tcaaatgccc gttattttat ggggataggg cttatcgcca cgggcattat
    40081 caacattctg tttggcttct cgacgtcgct atgggcgttt gccgtgctct gggtgctgaa
    40141 cgcctttttc cagggctggg gttcaccggt gtgtgcgcgt ctgttaacgg cctggtattc
    40201 acgtaccgag cgcggcggtt ggtgggcatt atggaacacg gcgcataacg tcggcggcgc
    40261 actcattccc attgtgatgg cagcggctgc actacattac ggctggcgtg ccgggatgat
    40321 gattgctggt tgtatggcga tcgtcgtggg aatttttctc tgctggcggc tgcgcgatcg
    40381 cccgcaggcg ttaggtttac cggcggtcgg tgaatggcga cacgacgcgc tggagatagc
    40441 tcaacaacaa gaaggggcag ggctgacgcg taaagagatc ctcaccaaat atgtgttgct
    40501 gaatccgtat atctggctgc tttcgttttg ctatgtgctg gtctatgtgg tccgggcggc
    40561 gatcaacgac tggggcaatt tgtatatgtc cgagacgctg ggcgtcgatc tggtcacggc
    40621 gaatacggca gtgacgatgt ttgaactggg tggatttatc ggtgcgctgg tagcgggttg
    40681 gggctcggac aaattgttta acggcaaccg agggccgatg aatttgattt tcgccgccgg
    40741 aattttgctt tcagtcggct ccctgtggct gatgccattt gccagctacg tgatgcaggc
    40801 aacctgcttc ttcaccattg gtttttttgt ctttggccca cagatgttaa tcggtatggc
    40861 ggcggcagag tgttcccaca aagaggcggc aggggcggcg acggggtttg tcggcttgtt
    40921 tgcttatctg ggggcgtcgc ttgctggttg gccgctggcg aaagtactcg atacctggca
    40981 ctggagcgga ttttttgtgg ttatctctat cgccgccggg atttccgcac tgctgttact
    41041 gccctttttg aacgcccaga caccgcgcga agcgtgatgc atctcacctt ttcacttcat
    41101 atccggcaaa actaagaaat tttccaggtt ttgcctggac gctatctcag gcctgatttg
    41161 ctgctgattt ttacaatgca tgcctcacgc aggtattcat ttcaggagta acccatgctg
    41221 gctttcttaa accaggttcg caagccgacc ctggaccttc cgctcgaagt gcggcgcaaa
    41281 atgtggttca aaccgttcat gcaatcctac ctggtggtct ttatcggcta cctgacgatg
    41341 tacctgattc gcaagaactt taacatcgcg cagaacgaca tgatttcgac ctacgggttg
    41401 agcatgacgc agctggggat gatcggcctg ggtttctcca tcacttatgg cgtgggtaaa
    41461 acgctggttt cctactacgc cgacggcaaa aacaccaaac aattcctgcc gtttatgctt
    41521 atcctgtctg ccatttgtat gctgggcttc agtgccagta tgggcagcgg ctcggttagc
    41581 ctgttcctga tgattgcctt ctacgcctta agcggctttt tccagagtac cggcggttcg
    41641 tgcagttact ccaccatcac caaatggacg ccgcgtcgta aacgcgggac attcctcggt
    41701 ttctggaata tttctcacaa ccttggcggt gcaggcgcag caggtgtggc gctgttcggg
    41761 gcaaattacc tgttcgatgg ccatgtcatc ggcatgttta tcttcccgtc gattatcgcg
    41821 ctgattgtcg gttttatcgg cctgcgttac ggtagcgact ccccggaatc ttatggcctc
    41881 ggcaaagctg aagaactgtt cggcgaggag atcagcgaag aggacaaaga gacagaatct
    41941 accgatatga ccaagtggca gatctttgtt gagtatgtgc tgaaaaacaa agtgatctgg
    42001 ctgctgtgct tcgccaacat tttcctctat gtggtacgta ttggtatcga ccagtggtca
    42061 accgtatacg cgttccagga actgaaactc tctaaagcgg tggcgattca gggctttacg
    42121 ctgtttgaag ctggtgcgct ggtcggtacg ctgctgtggg gctggctctc tgacctggcg
    42181 aacggtcgcc gtggcctggt ggcctgcatc gcgctggcgc tgattatcgc cacgctcggt
    42241 gtgtatcaac atgccagcaa cgaatatatc tatctggctt ctctctttgc gttgggtttc
    42301 ctggtctttg gcccgcaatt gttgattggt gtggctgctg ttggctttgt acctaaaaaa
    42361 gcgattggcg ctgccgatgg tattaaaggc acctttgctt acctgattgg tgacagcttt
    42421 gccaagttag gtctgggaat gattgccgat gggacgccgg tattcggcct taccggctgg
    42481 gcaggcacct tcgccgcact tgatatcgcc gcgattggtt gtatctgcct gatggcgata
    42541 gtggcggtga tggaagaacg caaaatccgc cgcgagaaaa aaattcagca gttgacagtg
    42601 gcataaacgt aactggtgac ttttgcccgg catgacgccg ggctttttta ttattccgtg
    42661 acttccagcg tagtgaaggc aaacttctcg ccatcaaata gcccctgact ggttagtttt
    42721 agcgcgggga tcactggcag agaaagaaac gccatctgaa taaacggctc atcgggtaac
    42781 ggaccgcatt cacgggcggc ggctttcaag gcgtcaattt gttccgccag caactgcgcc
    42841 gtgtcggtgc tcatcaggcc ggcaatgggc aacggcagat gactttggac ctggccgtta
    42901 cgtaccacgc acagcccgcc gccatcctga atcacctgat tgaccgccag cgccatctct
    42961 tcggcactgc gaccgatcac cacaatatta tggctgtcat ggctgaccgt cgcagccagc
    43021 gcaccttcat tcagtccaaa gccgccaagc aaaccacaag ccggagccag ccgttgcccg
    43081 taacgttcaa gtacggcaat aaagctcaca tcatcgcgat caaaaccatt ttcgctgtag
    43141 acgctggagt gggagtgcgt aatcaattcg ttatggatga cgtcaatgac ccgatagcgt
    43201 tttccgggcg taaattgcag ggcaaagtcg ctggcggaaa ctggctggcg ggcaatggtg
    43261 ttgccatatg gcggagcgga ttgtgccagt ctcgccgact cttccgcctg taaggtttgc
    43321 gcatcaatcg gctcgccttt caccagtacc tgctgcaccg tgaccttacg cgcatcgctc
    43381 aacaggacga tatcggcctg cttgccgggt gccagtaagc cgaggtgatt cagaccaaag
    43441 tggcgcgccg tcgaccagct ggcgacgcga tatgccacat gcagcggcac attgtgttgt
    43501 tcgatcaggc ggcgaattaa ggcatcgatg tgtccttcat gggcgatctc ccacgggtta
    43561 cggtcatcgg tacagagcat gcattgcggg ctgttaaatt cgttgatcaa cggtgccagc
    43621 gcgttgagat tgcgggcagc ggacccttcg cggatcatca acgacatgcc gagttgtaat
    43681 ttccggcgtc cttcttccag ctgataactt tcgtggcagt tttcaatacc cgcagtaata
    43741 taggcgttaa gttctttacc acccaaaccc gggcagtgac cgtccagcgt caggtggcga
    43801 aatgcatcca gtttatcgag cagcgcattc tgcccgctaa ttacgccagg gtagtccatc
    43861 atttctgcaa ggccggtaac ctgcggatgg tcccgccagg cgagcatctg ttcaagggta
    43921 aaactggcac cgttaacatc gcagccttcc agggcgggta cgcaagagct gacctgtaag
    43981 tactggtttt gccttgcctg ttcggcacag cgggcaaacc aggcgaatcc ggcttctccc
    44041 atcacgttga cgatttcatg agggtcgcaa ataacggtcg tcaggccgcg cggcagggta
    44101 gcggtttcaa aagtgaccgg cgtcatcatg ctggattcaa tatgcaggtg agcatcaata
    44161 aaccctggca ccgccgttgc gccgcgagca tcaatccgct gcaaagccgg agcatcagcg
    44221 tattctgcgc caacaccggc aatgtaacgt cctttaatca caattgggcc ggaaatttct
    44281 ccgccattga tcaggtcgag aatagagaca ttatcaataa tataatcggc aacagcgtcg
    44341 ccacgggaaa cggctaacaa ttcctggtat tcagcccggc taatgtgatg aaatttatgg
    44401 ttaatagaat tattcatatt tttctcattt tgattttatc aatttgatta ttatttatca
    44461 taaagaaacg tcgcgagatt attaaagaat atccattaat gtgcaattga aatgtgataa
    44521 ttatcactga accgttgttg tcatttgttt tacaaaagca agggattaaa tctcctccaa
    44581 tggacaaaaa aatgaataat gacaataccg attacgtgag taatgaatca gggacgcttt
    44641 cgcgattatt taaactacct cagcatggga ccaccgtccg cacagaattg attgcgggga
    44701 tgaccacttt tttaaccatg gtgtacatcg tttttgtgaa cccgcaaatc ctcggcgcgg
    44761 cacaaatgga cccgaaagtg gtgtttgtta ccacctgttt gattgccggt atcggcagta
    44821 ttgcgatggg gatatttgct aacttacccg tggcgctggc tccggcaatg gggctgaacg
    44881 ccttctttgc cttcgtggtc gtgggggcga tgggcatctc ctggcagacc gggatgggcg
    44941 caatattctg gggcgcagtt ggactatttt tgctcacgct gtttcgtatc cggtactgga
    45001 tgatctccaa cattccctta agtttacgta ttggtatcac cagcggaatt ggattattta
    45061 tcgccttaat gggattaaaa aatactggcg ttattgtcgc caataaagac acgctggtga
    45121 tgattggcga tttaagttct cacggcgtgt tgttaggtat tttagggttt tttattataa
    45181 ccgtgttgtc atcacgtcat tttcatgccg cggtgctggt ttctattgtg gtgacgtctt
    45241 gctgtggatt atttttcggt gatgttcatt ttagcggcgt ctattccatt ccgcctgata
    45301 ttagcggcgt cattggtgaa gtagatttga gcggcgcgtt aacacttgaa ctcgccggta
    45361 tcattttctc ctttatgctg atcaacctat ttgattcatc aggaacatta attggtgtaa
    45421 ctgataaagc gggcttaata gatagtaacg gtaaattccc caatatgaat aaggcgctgt
    45481 atgttgatag cgtcagttcg gtggcggggg cgtttatcgg cacctcgtct gttactgcct
    45541 atattgaaag tacttctggt gtggcagtcg gtggtcgcac ggggctgact gcggttgtgg
    45601 ttggcgttat gttcctgtta gttatgttct tctcaccgct ggtggcgatg gttcctcctt
    45661 acgcaaccgc cggagcgtta atctttgttg gcgtgctgat gacttcgagc ctggcgcgcg
    45721 ttaactggga tgattttacc gaatcggtgc ctgcgtttat taccacggtg atgatgccct
    45781 ttactttctc gatcaccgaa gggattgcac tcggctttat gtcgtactgc atcatgaaag
    45841 tatgcaccgg gcgctggcgc gatctgaacc tgtgtgtggt ggtggtcgca gctctgtttg
    45901 cactgaagat tattctggtg gattagtaac tggcgagggt ttccagatat catgagttct
    45961 gattacgcag gagaactcat gatctggata atgctcgcca cgctggcggt agtgtttgtg
    46021 gttggttttc gggtgctgac atccggggcc agaaaagcga ttcgccgtct cagcgatcgg
    46081 ctgaacatcg atgtcgtacc cgtggagtcg atggtcgatc aaatgggaaa gtcagctggt
    46141 gacgaatttt tacgttattt gcatcgtccg gatgagtcgc acctgcaaaa cgccgcgcag
    46201 gtgttgctca tctggcaaat tgtcattgtc gatggtagcg aacagaacct gctgcaatgg
    46261 catcggattt tacaaaaagc tcgcctcgcc gcgccgatta ccgacgctca ggtcaggctg
    46321 gcgctgggtt ttctgcgcga aaccgaacct gaaatgcagg atattaatgc ttttcagatg
    46381 cgctataacg cgttctttca gcctgccgag ggcgttcact ggttgcattg aaaccgtttc
    46441 gtcttatcag cctacagacc gcgcctgacc tgtcgggtgg ataagacgtt cacgccgcat
    46501 ccggtaatct ttgccctgtt ccgtttcgat cttaactcca gctcaagcaa caccgtaaaa
    46561 gttgcaaaaa cgttaaatgc gtcacacatt tcaatgttaa agtggtcggc ttttcccctg
    46621 aaacatgcca cgggtaacac catgagtgaa tttattgccg aaaaccgcgg cgcggatgcc
    46681 atcaaccgac cgaactggtc agccgttttc tcggtggcgt tttgtgtcgc ctgtctgatt
    46741 atcgttgagt ttttgcccgt cagtttgttg acgccaatgg cctaggattt aggcatttcg
    46801 gaaggggttg ccgggcaatc ggtgaccgtg accgcctttg tggcaatgtt tgccagtttg
    46861 tttattaccc agacaattca ggctactgac cgccgctacg ttgttatttt gtttgccgtt
    46921 ttgctgacgc tctcctgctt gctggtttcc tttgctaact cattcagttt gcttttaatc
    46981 ggtcgtgcct gtctggggct ggcgctgggc gggttctggg cgatgtcggc gtcgctgacc
    47041 atgcgtctgg tgccgccgcg tacggtgccg aaggcgctgt cggtgatctt cggcgcggtt
    47101 tctattgcgc tggtgattgc cgcgccgttg ggcagttttt taggcgagct tatcggttgg
    47161 cgcaatgtct ttaatgcggc ggcggtgatg ggcgtgctgt gtattttctg gattatcaaa
    47221 tcattgcctt cactgccagg cgaaccctcg catcagaaac aaaatacttt ccgcttatta
    47281 caacgtccgg gtgtgatggc agggatgatc gccatcttca tgtctttcgc cgggcagttt
    47341 gctttcttca cgtatattcg cccggtgtat atgaacctgg cgggattcgg cgtggatggc
    47401 ttaacgctgg tgctgttgag ttttggtatc gccagcttta ttggtacgtc gctttcgtcg
    47461 ttcattctta aacgttcggt aaaactggcc ttagcaggcg cgccgttaat actggctgtg
    47521 agtgcgttgg tactgacgtt gtggggaagc gataaaataa aatcgttgct accggcgtgg
    47581 cgattatctg ggggctaact tttgcattgg ttcccgtcgg ctggtcaacg tggatcaccc
    47641 gctcgctggc cgatcaggca gaaaaagccg ggtctattca ggtggcggtt attcagcttg
    47701 ctaatacctg tggcgcggca atcggcggtt atgcgctgga taatattggt ctgacttcgc
    47761 cgttgatgtt gtccggcaca ttgatgttgc tgactgcatt gttggttact gcaaaggtga
    47821 aaatgaagaa atcctgatat caggtaaaga agcgttggtc agtagttgtt gctgttctta
    47881 catatccggg cattctacct tacccaacgc ttcccaataa ttgctctgat tattacgttc
    47941 catcgccacg atggcctcgc gtatatgttg tttattgttt tcatctgaca acattaactt
    48001 ttcccatgct tcatctgata atgacgcctg tggcgtgcaa attttacgta aatcttgtaa
    48061 taccgtggtt ttctcttttg cggactgcag tgagctaaaa ggcgactcgg cgtatacgat
    48121 gcctggcata gcgaaaaaag tgaaaataag cagtagtgtc gttggtttca tggattcaac
    48181 cttttacaaa tgtgtatcga atgaagcata agtgtagttt gctttcatcg ctctcatcat
    48241 gctgtacgaa acgcgcttgc gtggtcagta agaagtgcca gactttatat tccactttta
    48301 ttccttttta ttcttatcga tagcgtttcg ttttttaaac cgcagcgacc ttaccgctat
    48361 agtcaggtaa tcattaataa aaggataaaa aaatgaaact gacaacacat catctacgga
    48421 caggggccgc attattgctg gccggaattc tgctggcagg ttgcgaccag agtagcagcg
    48481 atgcaaaaca cattaaagtt ggcgttataa atggcgcaga acaagatgtc gcggaagtcg
    48541 ctaaaaaggt ggcaaaagag aaatatggcc tcgatgttga actggtagga tttagcggtt
    48601 cgttgctacc gaacgatgcg acaaatcatg gcgaacttga tgcgaatgtc ttccagcatc
    48661 gaccctttct tgaacaagat aatcaggcgc atggttataa actggtagcg gtgggaaata
    48721 cgtttgtctt ccctatggcg ggttattcca aaaaaatcaa aaccgtggcg caaataaaag
    48781 aaggcgcgac agtggcgatc cccaacgacc cgaccaacct tggtcgcgca cttttactgt
    48841 tgcaaaaaga gaagctaatt actctgaaag agggaaaagg gttattacct accgcgctgg
    48901 atataaccga taatcctcgt catttgcaga ttatggaact cgagggggcg cagctcccgc
    48961 gcgtactgga tgatcccaaa gttgatgtag cgattatcag caccacttac attcagcaga
    49021 cagggctttc tccggtgcac gacagcgtat ttattgaaga taagaattcg ccgtatgtga
    49081 atattttggt ggcacgggaa gataataaga atgcagaaaa cgtgaaggaa tttctgcaat
    49141 cttatcaatc acccgaagtc gctaaagcgg cagaaaccat ttttaacggt ggcgcggtgc
    49201 ctggctggta attctcactc atgccgcgcc ggacgattta tttttctggc gcggcgacgg
    49261 gaatccattg aaatcaaaat caccgatgac agaatcagca gcgttcccag ccagtccggt
    49321 aatgtgaacg tgatccccag caacagcaaa gagagtagcg cgctacttaa tggttctgca
    49381 cagctcaaaa tgctggcttt tggaccgcca attaattgtg ctcctttcag gtacagacta
    49441 aatgtcaggg acgtaccaat gaccaccaaa taaaaaaacg ccagaatcaa actgccgtta
    49501 acgacaaagt ttgttccttg tctggcataa aaaggcaaca gaatcagacc gccaatcagc
    49561 atactccagc cgacgactgg taacgtgcca tagcgggcaa ttagcgttga gggataggtg
    49621 gtatagaatg cagcagcaaa tgccgaggca atgccccaga acaacgcggc aggagagatc
    49681 gataatgacg tcggattacc gtgtgtcacc aataaaaaag tcccgaccag cgatgtcaaa
    49741 atagcgcaga aaaccagaat gcccgggcgc gatttacgca ccagtgagaa ccaggcgacg
    49801 ataatcgtcg gtgagaggaa ttgcagcacc gttgccgtgg ctgcgttcga tttttcgatg
    49861 gttagcaaaa aagtgagctg tacagttagc gcgccaacca cggaaaaaat cagcaggcta
    49921 atggcatctt tatgattgtt aataatagaa aagattttat cgccatgaac aaatgacagc
    49981 gtcagtagaa tcaaaccggc gaatatcaaa cgcgtcatag tcaaaaactg cgacgacatc
    50041 tggctttgct ccatgatgta ttgcgcgcag acccctgaac ttccccacaa cacggcggca
    50101 atcagaacgt tcagcatccc ctttctggtg gaacccattt ttccctctgc atactgtttt
    50161 gtcgtttatg tatgcaggca tgatagcaaa aatggcgagg atggctctgt ttgcgccacg
    50221 ccgatttttc ttagcccaga ctggtgagct aaatatcttt aataaacagc aggcaaatca
    50281 tcgcaatacc caacatccct atcgccagcc agaacaacgc gtggtagctc cagatttcaa
    50341 ccataattcc gtcaacagag ccggcgataa tccagccgac gcgtgaagta tttgcatata
    50401 acgttgtcgc agagcctatt ttttcaggca tcaggtcctg aaaataaagc atgccgatac
    50461 cacagagaat accaaggaag atggcattta gaatttgcaa ttccagctca acagccggag
    50521 tcgtcgccat gagtacgctg gcgtaaaaac acattccact cacgatagca atgagcatta
    50581 atagccgctt gccaatacgt ttcatgtaat agcctgcgat taacatcatc ggaatttcca
    50641 gaccggcagc gataccaatc atttctccag ccagtttatc ggttagatgc agttcatcaa
    50701 taataaatag cggcatattt atcatgtaga gattattcgc cgcccacatc attgaacaga
    50761 ccacaaaaag tagccgcgta tcccgctttc tatggatgga gggtaaaatt tctacgggtt
    50821 gggtaacgac aggaatattt ctttgtattg atggcaaaaa caaccagact atcagcccgc
    50881 aaacaacaaa tgcgatggca gcggtgagat acatcacttt aaaactaaat cccattgcca
    50941 gttcataagc gagcggtggc ccgataaccc aggcaagcga gatctgagca cgtaaaaatg
    51001 tactgaacat aaccgtctca cggcctgttc tgtcggcgtg ttcacgggcg agggcgaaca
    51061 tttgcgggtt tgcggtagaa gcaaaactac tcagaagtac gcccgttgag agcagaatga
    51121 agtagttgct attccacgca aaaagcgtac aggccagcac tccaaataag caacatagca
    51181 ggatcagtaa tttacggtcg ccttgtttat cggagttcct tgccagaaat tgactgacca
    51241 gaattcccat aatagcgcta ccggtgaaga aaaaacctac cattatagga cgggctttca
    51301 gttcatctgc gaggaatata cttagggtag gagtctgaag agcgcccgca atacccgtca
    51361 gaaaggcgac aagtaaaaat gccgcggcag tgagatcaag tatttttgat ggagtggtag
    51421 ccgttttttg cattcttctg aatcaccggg cgaaaaaata taactgagtt acaaataagt
    51481 accagtcgaa tgagtattgt attaagaatt tttgagtatc gctgatacaa ccagaaagc
//
LOCUS       DBANUU010000013        51475 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000013 DBANUU010000000
VERSION     DBANUU010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.013"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            24..725
                     /locus_tag="ACIE68_000741"
     CDS             24..725
                     /locus_tag="ACIE68_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFX5907106.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            750..3350
                     /locus_tag="ACIE68_000742"
     CDS             750..3350
                     /locus_tag="ACIE68_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFX5907107.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVCDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            3341..4411
                     /locus_tag="ACIE68_000743"
     CDS             3341..4411
                     /locus_tag="ACIE68_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5907108.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4423..4965
                     /locus_tag="ACIE68_000744"
     CDS             4423..4965
                     /locus_tag="ACIE68_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5907109.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4973..5488
                     /locus_tag="ACIE68_000745"
     CDS             4973..5488
                     /locus_tag="ACIE68_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5907110.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5454..6191
                     /locus_tag="ACIE68_000746"
     CDS             5454..6191
                     /locus_tag="ACIE68_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFX5907111.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAIRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTWNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6302..7312
                     /gene="pyrD"
                     /locus_tag="ACIE68_000747"
     CDS             6302..7312
                     /gene="pyrD"
                     /locus_tag="ACIE68_000747"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFX5907112.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7486..8028
                     /gene="zapC"
                     /locus_tag="ACIE68_000748"
     CDS             7486..8028
                     /gene="zapC"
                     /locus_tag="ACIE68_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFX5907113.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8025..9134)
                     /gene="ycbX"
                     /locus_tag="ACIE68_000749"
     CDS             complement(8025..9134)
                     /gene="ycbX"
                     /locus_tag="ACIE68_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFX5907114.1"
                     /translation="MVTLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9378..11486
                     /gene="rlmKL"
                     /locus_tag="ACIE68_000750"
     CDS             9378..11486
                     /gene="rlmKL"
                     /locus_tag="ACIE68_000750"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFX5907115.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREVNEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11498..13405
                     /locus_tag="ACIE68_000751"
     CDS             11498..13405
                     /locus_tag="ACIE68_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFX5907116.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAVPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13535..14788
                     /gene="pqiA"
                     /locus_tag="ACIE68_000752"
     CDS             13535..14788
                     /gene="pqiA"
                     /locus_tag="ACIE68_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFX5907117.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14793..16433
                     /gene="pqiB"
                     /locus_tag="ACIE68_000753"
     CDS             14793..16433
                     /gene="pqiB"
                     /locus_tag="ACIE68_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFX5907118.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16430..16993
                     /gene="pqiC"
                     /locus_tag="ACIE68_000754"
     CDS             16430..16993
                     /gene="pqiC"
                     /locus_tag="ACIE68_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFX5907119.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            17249..17416
                     /gene="rmf"
                     /locus_tag="ACIE68_000755"
     CDS             17249..17416
                     /gene="rmf"
                     /locus_tag="ACIE68_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFX5907120.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17486..18004)
                     /gene="fabA"
                     /locus_tag="ACIE68_000756"
     CDS             complement(17486..18004)
                     /gene="fabA"
                     /locus_tag="ACIE68_000756"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFX5907121.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18073..19833)
                     /locus_tag="ACIE68_000757"
     CDS             complement(18073..19833)
                     /locus_tag="ACIE68_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFX5907122.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20019..20471
                     /gene="matP"
                     /locus_tag="ACIE68_000758"
     CDS             20019..20471
                     /gene="matP"
                     /locus_tag="ACIE68_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFX5907123.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20547..21599)
                     /gene="ompA"
                     /locus_tag="ACIE68_000759"
     CDS             complement(20547..21599)
                     /gene="ompA"
                     /locus_tag="ACIE68_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFX5907124.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQG
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(21956..22465)
                     /gene="sulA"
                     /locus_tag="ACIE68_000760"
     CDS             complement(21956..22465)
                     /gene="sulA"
                     /locus_tag="ACIE68_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFX5907125.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            22684..23313
                     /gene="sxy"
                     /locus_tag="ACIE68_000761"
     CDS             22684..23313
                     /gene="sxy"
                     /locus_tag="ACIE68_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFX5907126.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLLNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23276..25438)
                     /gene="yccS"
                     /locus_tag="ACIE68_000762"
     CDS             complement(23276..25438)
                     /gene="yccS"
                     /locus_tag="ACIE68_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFX5907127.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(25448..25894)
                     /locus_tag="ACIE68_000763"
     CDS             complement(25448..25894)
                     /locus_tag="ACIE68_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFX5907128.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26017..28071
                     /gene="helD"
                     /locus_tag="ACIE68_000764"
     CDS             26017..28071
                     /gene="helD"
                     /locus_tag="ACIE68_000764"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFX5907129.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDLWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28103..28561)
                     /gene="mgsA"
                     /locus_tag="ACIE68_000765"
     CDS             complement(28103..28561)
                     /gene="mgsA"
                     /locus_tag="ACIE68_000765"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFX5907130.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28657..29319)
                     /locus_tag="ACIE68_000766"
     CDS             complement(28657..29319)
                     /locus_tag="ACIE68_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFX5907131.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29492..29905
                     /locus_tag="ACIE68_000767"
     CDS             29492..29905
                     /locus_tag="ACIE68_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFX5907132.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29950..30267)
                     /gene="hspQ"
                     /locus_tag="ACIE68_000768"
     CDS             complement(29950..30267)
                     /gene="hspQ"
                     /locus_tag="ACIE68_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFX5907133.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30325..31515)
                     /gene="rlmI"
                     /locus_tag="ACIE68_000769"
     CDS             complement(30325..31515)
                     /gene="rlmI"
                     /locus_tag="ACIE68_000769"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFX5907134.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31610..31888
                     /gene="yccX"
                     /locus_tag="ACIE68_000770"
     CDS             31610..31888
                     /gene="yccX"
                     /locus_tag="ACIE68_000770"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFX5907135.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELADFRIR"
     gene            complement(31885..32214)
                     /gene="tusE"
                     /locus_tag="ACIE68_000771"
     CDS             complement(31885..32214)
                     /gene="tusE"
                     /locus_tag="ACIE68_000771"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFX5907136.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32305..32964)
                     /gene="yccA"
                     /locus_tag="ACIE68_000772"
     CDS             complement(32305..32964)
                     /gene="yccA"
                     /locus_tag="ACIE68_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFX5907137.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33171..33258)
                     /locus_tag="ACIE68_000773"
     tRNA            complement(33171..33258)
                     /locus_tag="ACIE68_000773"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33222..33224),aa:Ser,seq:tga)
     gene            33685..34803
                     /gene="hyaA"
                     /locus_tag="ACIE68_000774"
     CDS             33685..34803
                     /gene="hyaA"
                     /locus_tag="ACIE68_000774"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFX5907138.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGNQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34800..36593
                     /gene="hyaB"
                     /locus_tag="ACIE68_000775"
     CDS             34800..36593
                     /gene="hyaB"
                     /locus_tag="ACIE68_000775"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFX5907139.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            36612..37319
                     /gene="hyaC"
                     /locus_tag="ACIE68_000776"
     CDS             36612..37319
                     /gene="hyaC"
                     /locus_tag="ACIE68_000776"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFX5907140.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            37316..37903
                     /gene="hyaD"
                     /locus_tag="ACIE68_000777"
     CDS             37316..37903
                     /gene="hyaD"
                     /locus_tag="ACIE68_000777"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFX5907141.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLIIDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            37900..38298
                     /gene="hyaE"
                     /locus_tag="ACIE68_000778"
     CDS             37900..38298
                     /gene="hyaE"
                     /locus_tag="ACIE68_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFX5907142.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            38295..39152
                     /locus_tag="ACIE68_000779"
     CDS             38295..39152
                     /locus_tag="ACIE68_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFX5907143.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            39286..40830
                     /gene="appC"
                     /locus_tag="ACIE68_000780"
     CDS             39286..40830
                     /gene="appC"
                     /locus_tag="ACIE68_000780"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFX5907144.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFALKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            40842..41978
                     /gene="appB"
                     /locus_tag="ACIE68_000781"
     CDS             40842..41978
                     /gene="appB"
                     /locus_tag="ACIE68_000781"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFX5907145.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41991..42083
                     /gene="cbdX"
                     /locus_tag="ACIE68_000782"
     CDS             41991..42083
                     /gene="cbdX"
                     /locus_tag="ACIE68_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFX5907146.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            42163..43461
                     /gene="appA"
                     /locus_tag="ACIE68_000783"
     CDS             42163..43461
                     /gene="appA"
                     /locus_tag="ACIE68_000783"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFX5907147.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGAIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKMSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(43581..45761)
                     /gene="etk"
                     /locus_tag="ACIE68_000784"
     CDS             complement(43581..45761)
                     /gene="etk"
                     /locus_tag="ACIE68_000784"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFX5907148.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGINVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(45781..46227)
                     /gene="etp"
                     /locus_tag="ACIE68_000785"
                     /gene_synonym="yccY"
     CDS             complement(45781..46227)
                     /gene="etp"
                     /locus_tag="ACIE68_000785"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFX5907149.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(46215..47354)
                     /locus_tag="ACIE68_000786"
     CDS             complement(46215..47354)
                     /locus_tag="ACIE68_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFX5907150.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(47400..49496)
                     /locus_tag="ACIE68_000787"
     CDS             complement(47400..49496)
                     /locus_tag="ACIE68_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFX5907151.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGHWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(49496..50242)
                     /locus_tag="ACIE68_000788"
     CDS             complement(49496..50242)
                     /locus_tag="ACIE68_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFX5907152.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(50239..50883)
                     /gene="gfcB"
                     /locus_tag="ACIE68_000789"
     CDS             complement(50239..50883)
                     /gene="gfcB"
                     /locus_tag="ACIE68_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFX5907153.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(50990..51295)
                     /locus_tag="ACIE68_000790"
     CDS             complement(50990..51295)
                     /locus_tag="ACIE68_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5907154.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
BASE COUNT        12729 a        12706 c        13557 g        12483 t
ORIGIN      
        1 atatttttta agagtgacta tttatgaaaa cttgcataac aaagggaatt gtgaccgtaa
       61 gtttaacggc aatattactc agctgctcat ccgcatgggc ggcaggtaaa ggcgggattg
      121 gacttgcagc aacacgtctt gtttattcag aaggtgaaga gcaaatttca ctgggtgtac
      181 gtaacaccag tccggatgtt ccttatctta ttcagtcatg ggtgatgacc ccagataata
      241 aaaaatcagc agattttatt attacaccac cgctatttgt gctgaatccg gctaatgaga
      301 atctgttacg cattatgtac attggtgcgc cgttggcgaa agacagagaa acccttttct
      361 tcactagcgt acgggcagtc ccttcaacaa cgaagcggga agagggaaat accctgaaga
      421 ttgccacaca aagcgtcatc aaacttttct ggcgaccaaa aggtttagcg tatcccttag
      481 gcgaggctcc ggcgaaactg cgttgcactt cgtcagctga catggttacg gtcagtaacc
      541 caacacctta tttcattacc ctgacagacc tgaaaatagg tggaaaagta gttaaaaatc
      601 aaatgatttc cccctttgat aaataccaat tttctctgcc aaagggggcc aaaaatagca
      661 gcgtaacgta tcgaaccatc aatgactacg gggcggaaac gccgcaactc aactgtaaat
      721 cgtaagccgt cttcagttaa gagagcgaga tgtatagaac tcatcgacaa cacagcctgt
      781 taagctctgg tggagtgcca tcgtttattg gtgggctggt ggtgtttgtg tcggcagcgt
      841 tcaatgcaca agctgaaacc tggttcgatc ctgctttttt caaagatgat ccctcaatgg
      901 tggccgattt gtctcgtttc gaaaaaggac aaaaaataac gccaggggtt tatcgtgtcg
      961 atattgttct gaatcagaca attgtagata cgcgcaacgt caattttgtt gagataacgc
     1021 cagagaaggg gattgccgcc tgtttgacga ctgaaagcct ggatgcaatg ggtgttaata
     1081 ctgatgcgtt tccggctttt aaacaactgg acaaacaagc gtgtgtgcca ttggcggaga
     1141 ttattccgga tgccagcgta acgtttaatg tgaataaact ccgtctggaa atttcagtac
     1201 cgcaaatcgc catcaaaagt aatgcccgtg gttatgtccc ccctgaacgt tgggatgaag
     1261 ggatcaacgc gctattactg ggatattcgt ttagcggggc taacagtatt catagcagcg
     1321 cagacagtga ttctggcgac agctattttc tgaatttaaa cagtggcgtt aatttaggcc
     1381 catggagatt gcgcaacaat tcaacatgga gtcgcagtag tggccaaacc gcagaatgga
     1441 agaatctcag cagctatttg cagcgggcgg ttattccact aaaaggcgaa ctgaccgtat
     1501 gtgatgatta tactgcaggc gattttttcg atagtgtcag ctttcgtggt gtgcagctgg
     1561 cgtcagatga caacatgctg ccagacagcc tgaaagggtt tgcgcctgtg gtgcgtggta
     1621 tcgccaaaag caatgcccag ataacgatta agcaaaatgg ttacaccatt taccaaactt
     1681 atgtatcgcc tggtgctttt gaaattagtg atctctattc cacgtcgtcg agcggtgatt
     1741 tgttagttga aatcaaagaa gcggacggca gcgtcaatag ctacagcgta ccgttttcca
     1801 gcgtgccatt actccagcgt caggggcgaa tcaaatacgc ggtgacactg gcgaaataca
     1861 gaaccaatag taatgaacag caggagagca aatttgccca ggccacgttg cagtggggcg
     1921 gaccgtgggg aacgacatgg tatggtggtg gacaatatgc tgaatattac cgtgccgcca
     1981 tgtttggtct gggatttaac cttggcgatt tcggagcaat ttcgttcgat gcgacccagg
     2041 cgaagagtac gctggcagac caaagcgaac ataaaggtca gtcatatcgt tttctgtatg
     2101 ccaaaacgct caaccaattg ggcactaatt ttcaattgat gggctatcgc tattcgacgt
     2161 cgggtttcta caccctttcc gacaccatgt ataaacatat ggatggctac gaatttaatg
     2221 acggtgatga tgaagatacg ccgatgtggt cgcgttatta caatttgttt tacaccaaac
     2281 gtggcaaact gcaggtcaat atctcccagc aattaggcga gtacggttcg ttttatttaa
     2341 gtggtagcca gcaaacttac tggcataccg atcaacagga tcggctatta cagtttggct
     2401 acaacacgca aattaaagat ctctcgctgg gggtttcctg gaactacagt aagtcccgtg
     2461 gtcaacctga tgctgatcag gtgtttgcac taaatttttc cctgccgctc aatctgttgc
     2521 tccccagaag taatgatagc tataccagga aaaaaaatta cgcctggatg acctctaaca
     2581 ccagtatcga taacgaaggg cacattacac aaaacctggg tttaacggag acactactcg
     2641 atgacggtaa cctgagctac agcgtgcaac agggatataa cagcgagggg aaaacggcta
     2701 atggtagcgc cagcatggac tacaaagggg cgtttgcaga tgcccgagtg ggctacaact
     2761 acagcgataa cggcagtcaa caacaactga actacgctct ttcaggcagt ttagttgccc
     2821 attcacaggg cattaccctg gggcaatcgc tgggggaaac taacgtgctg attgcagcac
     2881 caggcgcaga gaatactcgt gtggcgaaca gcaccgggct gaaaactgac tggcgcggat
     2941 ataccgttgt cccctatgcc acttcttatc gggaaaatcg aatcgcactt gatgcggcat
     3001 cgttaaaacg taacgtggat cttgaaaatg cagtagtcaa cgtggttccc accaaagggg
     3061 cgttggttct ggcggagttc aatgcccatg cgggagcaag ggtattaatg aaaacatcaa
     3121 agcagggtat accgctgcgt tttggcgcga tagcgacgct ggacggcata cagactaata
     3181 gcggcattat tgatgatgat ggctcgctct atatgtccgg tttgcccgct cagggagcga
     3241 taactgtacg ctggggcgaa gctcccgatc aaatttgtca tatcagttac cagcttaccg
     3301 aacaacaaat taactctgcg attacgcgaa tggatgccat atgcagataa tctttggaga
     3361 aaaatgcgtg ttattactac gactattttt tgccgccgtc ttaatgctat ggtgcgctca
     3421 aactgctgct tatagcgggc agtgtcatac cactcagggg aatccgtata ttggcgtcaa
     3481 ttttggcgtt aaaaccctgg aggaagaaga aaatacggct ggagtagtta aagacaaatt
     3541 ttatcagtgg aacgaatcga atgattatta tgtttcctgt gattgcgata aagacaatgt
     3601 cagaagtggc cgatgggcat tcgccgcgga ttcaccgtta gtctatttag gcgacaactg
     3661 gtacaaaatt aatgactatc ttgccgccaa agttttattg caggttaaag gcagttctcc
     3721 tactgcggtt cctttcgaaa acgtgggcac aggggcagat acacgatggc atatttgcga
     3781 tcccggcggt caacgtttag gtggccaggg ggctagcggt aatagcggta gcttttccct
     3841 gaaaatattg cagccgttcg ttggttcggt cgtcattcct cctatggcgc tggcgcgatt
     3901 atttgaatgc tacaacatac ccgcaggtga ttcctgcacg actacaggta caccggtttt
     3961 agtgtattac ctgtctggta cgatcaattc acttggctca tgttccgtca atgccggaga
     4021 aacaatcgag gtcgatctgg gcgacgtatt tgcggctaac tttcgtgttg tagggcataa
     4081 gcctcttggg gccagaacgg cagaacttgc aattccagtc aggtgtaaca cgggaaacgc
     4141 ggggttagtt aacgtcaacc tgagtctgac ggcaaccaca gaccccagct atccccaggc
     4201 gattaagacg tcacgtcctg gcgtgggcgt ggtggtgacc gatagccaga acaacattat
     4261 ttcccctgct ggtggaacat taccgctctc tattcctgat gatgcagaca gtatcgcgcg
     4321 aatgaatgtc tatccagtca gcacgacagg tgtaccacca gaaaccgggc gatttgaagc
     4381 cacggcaacg gtgagaataa attttgatta acgcgtgaac gtatgaagaa aaaaacgata
     4441 tttcagtgcg ttatcttgtt ctttagcatt cttaacatcc atgtcgggat ggctgggcct
     4501 gaacaagtta gtatgcatat ttatgggaat gtggtcgatc agggctgtga tgtcgccacc
     4561 aaaagtgcat tacaaaatat tcatattggt gattttaata tcagtgattt tcaggccgcg
     4621 aataccgtaa gcactgctgc tgatttgaat atcgatatca ccggttgtgc cgctggtatt
     4681 actggcgcgg acgtcctttt tagcggcgag gctgacaccc ttgcgccgac actgctcaaa
     4741 ctaactgaca caggcggaag cggtggtatg gcaacgggga ttgccgtgca aattcttgat
     4801 gcgcaaagtc agcaagaaat cccgctcaat caggtccagc ctcttacgcc cttaaaagcc
     4861 ggggataaca cactcaaata tcaacttcgt tataagtcca caaaggcggg agcaacgggc
     4921 ggtaatgcga cggcggttct ctattttgat ctggtttacc agtgaaggag tgatgttgaa
     4981 acggataatc tggattctgt tcttactggg attaacgggg ggctgtgagc tatttgccca
     5041 tgatggcacg gtcaacatta gcggatcgtt tcgccgtaat acatgcgtgc tggcacagga
     5101 tagcaagcaa atcaatgtgc agttaggcga tgtttcgctg acacgttttt ctcatgggaa
     5161 ttatggcccg gagaaatctt tcatcattaa tttgcaggat tgcggaactg atgtcagcac
     5221 ggttgacgtc accttttcag gaacccccga cggagtgcag agcgaaatgc tgtccattga
     5281 aagtggtacg gatgcagcca gcgggctggc aattgcgatt ctggatgatg cgaagatatt
     5341 gattccgctt aaccaggcga gtaaagacta cagcctacat agcggtaagg tgccattaac
     5401 tttttatgcg caattgcgac ctgtcaatag cgatgtgcag tcgggtaaag tgaatgccag
     5461 cgcgacattt gtacttcatt atgactaata cgtggaacag attggcgctc ttgattttcg
     5521 ccgttttatc gctgctggtg gcgggtgaat tgcaggcagg agtggtggtc ggcggaacgc
     5581 gatttatctt tccggcagac agagaatcga tatctatttt actgactaat accagtcagg
     5641 aatcctggct tattaacagc aaaatcaacc gcccaacgcg ttgggccggg ggggaagcgt
     5701 cgacagtgcc agcaccatta ctggccgctc cgccacttat tctcctgaag cccggtacta
     5761 caggcacgtt gcgcttgctg agaacggaaa gcgacatctt gcctgtggat cgcgaaacgc
     5821 tatttgagtt aagcattgcc agcgtgccat ccggcaaagt tgaaaatcag agcgtaaaag
     5881 tggcgatacg ctcggtattt aaactgttct ggcgacccga aggtttgccg ggcgacccgc
     5941 tggaagctta tcaacaatta cgctggacat ggaattcgca gggtgtacaa ctcactaacc
     6001 caacgcctta ctacattaac ctgattcagg tgagcgtaaa tggtaaagcg ttaagtaatg
     6061 cgggagttgt gccgcctaaa agccagcgtc agacaagctg gtgtcaggca atcgcaccgt
     6121 gtcatgtcgc ctggcgggcg ataaatgatt acggcgggtt gagtgcaaag aaggagcaaa
     6181 atctgccctg aaacaggttc ggaaaacgtt tgcgtttttt ttgccgcagg tcaattccct
     6241 tttggtccga actcgcacat aatacgcccc cggtttgcac accgggaatc caggagagtt
     6301 catgtactac cccttcgttc gtaaagccct tttccagctc gatccagagc gcgctcatga
     6361 gtttactttt cagcaattac gccgtattac aggaacgccg tttgaagcac tggtgcggca
     6421 gaaagtgcct gcgaaacctg ttaactgcat gggcctgacg tttaaaaatc cgcttggtct
     6481 ggcagccggt cttgataaag acggggagtg cattgacgcg ttaggcgcga tgggatttgg
     6541 atcgatcgag atcggtaccg tcacgccacg tccacagcca ggtaatgaca agccgcgtct
     6601 ctttcgtctg gtagatgccg aaggtttgat caaccgtatg ggctttaata atcttggcgt
     6661 tgataacctc gtagagaacg taaaaaaggc ccattatgac ggcgtcctgg gtattaacat
     6721 cggcaaaaat aaagatacgc cagtggagca gggcaaagat gactatctga tttgtatgga
     6781 aaaaatctat gcctatgcgg gatatatcgc catcaatatt tcatcgccga ataccccagg
     6841 attacgcaca ctgcaatatg gtgaagcgct ggatgatctc ttaaccgcga ttaaaaataa
     6901 gcaaaatgat ttgcaagcga tgcaccataa atatgtgccg atcgcagtga agatcgcgcc
     6961 ggatctttct gaagaagaat tgatccaggt tgccgatagt ttagttcgcc ataatattga
     7021 tggcgttatt gcaaccaata ccacactcga tcgttctctt gttcagggaa tgaaaaattg
     7081 cgatcaaacc ggtggcttaa gtggtcgtcc gcttcagtta aaaagcaccg aaattattcg
     7141 ccgcttgtca ctggaattaa acggtcgctt accgatcatc ggtgttggcg gcatcgactc
     7201 ggttatcgct gcgcgtgaaa agattgctgc gggtgcctca ctggtgcaaa tttattctgg
     7261 ttttattttt aaaggtccgc cgctgattaa agaaatcgtt acccatatct aattatttct
     7321 tcgaattcct ctttataaga taatcagggc tttattttca gccctggttg ttttatattc
     7381 attgctgttg cttatttaga cattttgtac ttttattgtt gaggttatta agcgaagcga
     7441 caatggattg tgttgctgcg gttttatagg gtaggggaga ggcagatgcg aattaaacca
     7501 gacgataact ggcgttggta ttacgatgaa gagcacgatc gaatgatgct cgatttagcc
     7561 aatggtatgc tatttcgctc acgttttgcg cgcaagatgt tgactccaga tgccttttct
     7621 cccgcaggct tttgcgttga cgacgccgcg ctctatttct cttttgaaga aaagtgccgc
     7681 gattttaatt tatctaaaga acaaaaagcc gaactggtgc tgaatgcact ggtagcaatt
     7741 cgctatctca aaccgcaaat gccgaaaagc tggcattttg tttcccatgg tgaaatgtgg
     7801 gtgcccatgc cgggcgatgc ggcctgcgtt tggttgagcg atactcacga gcaggtcaat
     7861 ttgctggtcg ttgaatctgg cgaaaatgcc gcactatgcc tgctggcaca accctgcgtt
     7921 gttattgcgg gtcgcgcgat gcagttgggc gacgccatta aaatcatgaa cgacaggctg
     7981 aaaccgcagg ttaatgttga cagcttcagc ctcgaacagg cagtctaacg cgccaactta
     8041 agtgcagtct tcggtacaca gctacagcaa agaatggtgc catcatcgcc cattgctgat
     8101 tttttcagcg gcgtgacttc gccttctaaa agctgaacac ggcaacttcc acaaatgccc
     8161 gcgcggcaag aataagggat acgaattccc tgattttcta attgctccag caacacctgt
     8221 tggttatttc cacgaaatgc ctgtccctgc cagtcaatat ctacatttgc gtccggttgt
     8281 tgcgtgatgt tggcggtatc atcagcggca gctgcgccgt aaatttttgc cggagccgtt
     8341 gccagaattt ccacctcatc gccaacgcga atcacgccgc tattacgggc aattaaattc
     8401 tgaccaaaat cgacatcgcc gttatcctgg gcagtgcgga aagattgcaa tgtttttaat
     8461 ggttcgcctg ccggatgttt ttgccctttt tctgggctga cggtggtgaa aatacagcgg
     8521 ctacaaggtt taaccacatc aaacaccaca tcaccaatgc gaatcacttt ccagcgatct
     8581 tcttcccagg ctgacgcgcc ggaaaccacc agattggggc ggaattgctc catttttaca
     8641 ctggccggac aacgttgttg gagatcacgt aacgaggctt cgttagcaag aaggtaagga
     8701 tagccatcag caaatgacag aggtacagtg ttgtggcgtt tcacgcgccg ggtcatttgt
     8761 ggccccaccc agcgtaattg cacttcgcgg gagaaaaatc cacttagcca tttgttgatc
     8821 gcgtctggcg caattcgcgc ggtaaaatgt gtgccccaaa cttcggttgg tgcgtcttgt
     8881 gtggcgaaat cagcaaaacg aacatatgcg ctactgccat ctggtgcggt gagatgcaag
     8941 ccatcatgca caggtgaagg agtaaaccgt accatctggg gaaactggcg agcggtaata
     9001 aacgtaccgt caggttccgt gatcataaag atgcgatcga aggccagacc actgacatca
     9061 gccagagcat gtgtaagacc aatgccgcgc atcgatttaa caggatgaat aaaaagccgg
     9121 gttaatgtca ccacggtgcg gtcctcaaat gaaaataagc cctcaacttt atgacatgac
     9181 gcgcttatta gctataatgc gcaacaattt tcttagcgcc tgtcccatta ggctatttta
     9241 tttgtcagtt tggccctggg ttgtgctcga aatcctcacg tacttgtgta cgctccggtt
     9301 tctccgcgct gtccatgtcc agactgactg caacaattac gcctactgcg gtaggctctt
     9361 agagtaaaag tgacgatatg aattctctgt ttgccagtac ggcccgtggg ctggaagagc
     9421 tgttaaaaac tgaactggaa aacctggggg ccgttgaatg ccaggtggtt cagggtgggg
     9481 tccatttcaa gggcgacaca cggcttgttt accagagcct gatgtggagc cgcctggcct
     9541 cgcgtattat gttgccgctg ggcgagtgta aggtttacag cgatttagac ctctatctcg
     9601 gtgttcaggc gatcaactgg acagagatgt ttaatcctgg cgcgaccttc gctgtccact
     9661 tcagtggttt gaatgacacc atccgcaaca gtcagtacgg cgcgatgaaa gtgaaagacg
     9721 cgatcgtcga tgctttcacg cggaaaaatc tgccgcgtcc aaatgttgat cgcgatgcgc
     9781 cggatatccg cgttaacgtc tggctgcata aagaaaccgc cagtatcgcc cttgatctca
     9841 gtggtgatgg tttacatctg cgtggctatc gcgatcgtgc tggtattgcg ccgatcaaag
     9901 aaaccctggc agccgctatt gtgatgcgat ccggctggca gccaggaaca ccgctgctcg
     9961 atccgatgtg tggttccggt acgttgctga ttgaagcagc gatgctggcg accgatcgcg
    10021 cacctggctt gcaccgtggg cgttggggct ttagcggctg ggcgcagcat gatgaagcta
    10081 tctggcagga agtgaaagcg gaagcgcaaa ctcgcgcccg taaaggcctg gctgagtata
    10141 gctctcattt ttacggttcg gacagcgacg cacgggtgat tcaacgtgcg cgcactaacg
    10201 cccgtcttgc ggggattggt gaactgatca cctttgaggt gaaagatgtc gcgcaactga
    10261 ccaatccgct gccgaaaggg ccgtacggta cagtgttgag caacccgcca tacggtgaac
    10321 gtctggacag cgaaccggcg ctgatagcgc tgcatagtct gctgggccga atcatgaaaa
    10381 accagttcgg tggctggaat ctctctttgt ttagtgcctc gccggatctg ctcagctgcc
    10441 tgcagctgcg tgcagacaaa cagtacaagg cgaaaaacgg cccgctggac tgcgtacaga
    10501 aaaattacca cgttgccgaa agcactccag acagcaaacc ggcgatggta gcggaagact
    10561 acgctaaccg cctgcgtaag aacctcaaaa aattcgagaa gtgggctcgc caggaaggga
    10621 ttgaatgtta ccgcctgtat gacgccgatc tgccggaata taacgttgcc gttgaccgtt
    10681 atgccgactg ggtggtggtg caggagtatg cgccgccaaa aactattgat gcgcacaaag
    10741 cgcgtcagcg tctgttcgat attatcgctg caaccatttc ggtactgggg attgcgccaa
    10801 acaaactggt gctgaaaacc cgtgaacgcc agaagggcaa aaatcagtac cagaaactgg
    10861 gcgagaaggg cgagtttctc gaagtcaccg aatataacgc tcacttgtgg gtgaacctga
    10921 cggattacct cgataccggt ctgttcctcg atcaccgtat cgcccgtcgt atgctcggtc
    10981 agatgagcaa aggcaaagat ttcctcaacc tgttctctta taccggcagc gccaccgtgc
    11041 acgcgggatt aggcggtgca cgcagcacca ccaccgtgga tatgtcgcgt acttatctgg
    11101 agtgggcaga acgcaacctg cgtctgaatg gcctaaccgg gcgtgcgcat cgcctgattc
    11161 aggccgattg cctggcgtgg ctgcgtgagg taaatgaaca gttcgatctg atctttatcg
    11221 atccgccaac cttctctaac tcaaaacgaa tggaagatgc gtttgatgtt cagcgcgatc
    11281 atctggcgct gatgaaagat ttgaaacgtc tgctgcgtgc tggtgggacg atcatgttct
    11341 cgaacaacaa acgtggcttc cgtatggatc tcgacggcct ggcaaaactg ggactgaaag
    11401 cacaagaaat tacgcaaaaa acgctctccc aggatttcgc ccgtaaccgc cagatccaca
    11461 actgctggct gattaccgca gcctgaaagg aatagtaatg tcattaatca gtatgcatgg
    11521 cgcatggctg tcgttcagcg acgcgccgct tctcgataac gcagaactgc atatcgaaga
    11581 taacgaacgt gtttgtctgg tgggccgcaa cggcgcaggc aaatcgacgt taatgaaaat
    11641 cctcaaccgt gaacaagggc tggatgacgg tcgcattatt tacgagcagg atttgattgt
    11701 agcgcgtctg caacaggatc cgccgcgtaa cgttgagggt agtgtttatg atttcgttgc
    11761 cgaaggcatt gaagaacaag cggaatatct gaaacgctat cacgatattt ctcgcctggt
    11821 gatgaacgac ccgagcgaga aaaatctcaa cgaactggca aaggttcagg aacagctgga
    11881 tcaccacaac ctgtggcagc tggaaaaccg catcaacgaa gtgctggcgc aactggggtt
    11941 agatcctaac gttgcgctgt cgtcgctttc cggcggctgg ttgcgtaaag cggcattagg
    12001 acgcgcgctg gtgagcaatc cgcgcgtatt gctgctggac gaaccgacta accacctgga
    12061 tatcgaaacc atcgactggc tggaagggtt cctcaaaacc ttcaatggga cgattatttt
    12121 catctcccac gaccgttcgt ttatccgcaa tatggcgacg cgcattgttg atctcgatcg
    12181 cggcaagctg gtgacctatc cgggcaatta cgatcagtac ctgctggaaa aagaagaagc
    12241 cctgcgcgtg gaagaactac aaaatgccga gttcgatcgc aaactggcgc aggaagaagt
    12301 gtggattcgc caggggatca aagcacgccg tacccgtaat gaaggccgcg tacgcgccct
    12361 gaaagcgatg cgtcgcgagc ggggcgaacg tcgcgaagtg atgggtaccg caaagatgca
    12421 ggtggaagag gccagccgct ccggtaagat cgttttcgaa atggaagacg tttgctatca
    12481 ggttgacggt aagcaactgg tgaaagattt ttctgcccag gttctacgtg gcgacaaaat
    12541 tgccctgatt ggtccgaatg ggtgcggcaa aaccacgctg ctgaaactaa tgctcggtca
    12601 gcttcaagca gacagcgggc gtattcacgt tggcaccaaa ctggaagtgg cttatttcga
    12661 tcagcaccgc gcggaactgg atcccgataa aacggtgatg gataaccttg ccgaaggtaa
    12721 gcaagaggtg atggttaacg gcaagccacg ccacgtattg ggctatttgc aggacttcct
    12781 gttccatcca aaacgggcga tgacgccggt acgtgcgctt tctggcggtg agcgtaaccg
    12841 cttgttgttg gcgcgtttgt tcctcaaacc aagcaactta ttgattcttg acgaaccgac
    12901 caacgatctt gatgtcgaaa cgctggaact gctggaagaa ctgatcgaca gctatcaggg
    12961 cacggtattg ctggttagcc acgatcgtca gtttgtcgat aacaccgtta cagaatgctg
    13021 gatcttcgaa ggcggcggta aaattggtcg ttatgttggc ggttatcatg atgcccgtgg
    13081 tcagcaagaa cagtatgtgg cgctcaaaca gcctgcggtg aaaaaaaccg aagaagccgc
    13141 cgtgccaaaa gcagaaactg taaaacgcag cagtagcaaa ctaagctata aattgcagcg
    13201 cgaactggag cagctaccgc aattgctcga agatctggag gcgaagctgg aagccctaca
    13261 gacgcaggtg gcggatgctt ccttcttcag tcagccgcat gagcagacac aaaaagtgct
    13321 cgccgatatg gctgctgcag agcaggagct ggagcaagcc tttgaacgct gggagtatct
    13381 tgaagcgtta aaaaatggtg gctgatcgca aaatagtcga tttaaacaga gacagctgac
    13441 aacggtaaaa ttgcctgatg aactgggttt atcaggcatg aaaaccaaca ctttgttagt
    13501 catctgtaaa atagcgcatc attaaggagt accaatgtgc gaacatcatc atgccgcgaa
    13561 gcacatcctg tgctcgcagt gtgacatgct ggtggcgtta ccgcgccttg agcatggtca
    13621 gaaagcggca tgtccccggt gtggcacaac gttaaccgtg gcgtgggatg cccctcggca
    13681 gcgtccgacc gcctatgcgt tggctgcact gttcatgctg ttgctgtcca acttgtttcc
    13741 ttttgtgaat atgaacgttg caggagttac cagtgaaata acattactgg aaattcccgg
    13801 cgtgcttttt tctgaggact acgccagcct cggcaccttt ttcctgttgt ttgtgcaact
    13861 ggttcccgcg ttttgtctga taaccattct gttactggtg aatcgcgcgg aattaccggt
    13921 ccgtttaaaa gagcaactgg cacgggtgct ttttcaactc aaaacctggg gaatggcgga
    13981 gattttcctc gctggtgtgc tggtcagttt cgttaaactg atggcttacg gcagcattgg
    14041 ggtaggcagc agctttctcc cctggtgttt attttgtgtc ctgcaactgc gcgcttttca
    14101 gtgcgttgat cgtcgctggt tatgggacga catcgccccg atgccagaac tgcgccagcc
    14161 gctaaaacca ggcgtcacgg ggatacgtca ggggctgcgt tcgtgctcct gttgtacggc
    14221 aatccttcct gctgatgaac ccgtgtgccc gcgttgtagt accaaagggt acgttcggcg
    14281 tagaaacagc ctgcagtgga cactcgcgct gcttgtaacg tccatcatgc tgtatcttcc
    14341 ggctaatatt ttgcccatca tggtgacgga tttattaggc tcgaagatgc cgtcgacgat
    14401 tctcgctggg gtcattctgt tatggagcga aggctcttat cccgtcgctg cggtgatctt
    14461 tctggccagt attatggtgc caacgttaaa gatgatcgcc atcgcgtggc tgtgttggga
    14521 tgccaaaggg catggcaagc gcgacagtga aagaatgcat ttgatttatg aagttgttga
    14581 gtttgtaggc cgctggtcga tgattgacgt tttcgttatc gcggtgctct cggcgctggt
    14641 gcgtatggga ggtttaatga gtatttatcc ggcaatgggt gcattaatgt ttgctttagt
    14701 cgtcataatg acaatgtttt ctgctatgac gtttgacccg cgtttgtcgt gggatcgtca
    14761 acctgaatca gagcatgagg agtcctgaca gtatggaatc taataatggg gaagccaaaa
    14821 tccagaaagt gaagaactgg tctcccgtgt ggatatttcc tatcgtcacg gcgctcattg
    14881 gggcctgggt tcttttttat cattacagcc atcagggacc ggaagtgacc ctgatcaccg
    14941 cgaatgcgga aggaattgaa ggtggcaaaa ccaccattaa aagccgtagc gttgacgtcg
    15001 gcgtggttga aagcgccaca ctggctgatg atttgacgca cgttgaaatc aaagcgcggc
    15061 tgaattccgg tatggaaaaa ttgctgcata aagacaccgt cttttgggtg gtgaaaccgc
    15121 agattggtcg cgaagggatt agcggcctgg gaacgctgct gtctggagtt tatatcgaac
    15181 tgcagccagg cgcgaaaggc agcaaaatgg ataaatacga tttgctggac tcgccaccgt
    15241 tggccccgcc tgatgcgaaa ggtatccgtg tgattctcga tagcaaaaaa gccgggcagc
    15301 tctcgccagg agatccggtg cttttccgtg gctatcgggt aggttcggtt gaaaccagca
    15361 ccttcgatac gcaaaaacgc aatatcagtt atcaactgtt catcaatgca ccttatgacc
    15421 gactggtgac cagcaatgtt cgcttctgga aagatagtgg cattgcggtt gatctgacgt
    15481 cagcggggat gcgtgtggag atgggctcat tgacaacgct gcttagtggc ggtgttagct
    15541 ttgatgtgcc ggaaggtctg gatttagggc agccagtggc accgaaaaca gctttcgttt
    15601 tgtatgatga tcagaagagc attcaggatt cgttgtacac cgatcacatt gattatctga
    15661 tgttctttaa agattcggta cgcggtctgc aaccgggagc tccggtagaa ttccggggta
    15721 ttcgcctggg taccgtaagc aaagtgccat tctttgcgcc gaatatgcgt cagacattta
    15781 acgatgatta ccgtattccg gtactgattc gtatcgagcc agagcggctg aaaatgcagc
    15841 ttggcgaaaa tgcggatgtt gttgagcacc ttggcgaatt gttgaaacgt ggtttacgcg
    15901 gatcgctgaa aaccggaaac ctggtcactg gtgcactgta tgttgatctc gatttctatc
    15961 caaatacgcc tgcaataacc ggtattcgtg aatttaatgg ttatcagatt atcccgactg
    16021 ttagcggcgg cctggcgcaa atccagcaac gactgatgga agcgttggat aagatcaaca
    16081 aactgccatt gaatccgatg attgaacagg caaccagtac gctttctgaa agtcagcgca
    16141 caatgaaaaa cctgcaaacg acgctggata gcatgaacaa gatcctcgct agccagtcga
    16201 tgcagcagtt gccgacggat atgcagtcaa cgttgcgtga attgaatcgc agcatgcagg
    16261 gcttccagcc tggctccgca gcctacaaca agatggtggc ggatatgcag cgccttgatc
    16321 aggtgttgcg agaactgcaa ccggtgctga aaacgctcaa tgagaagagt aacgcgctgg
    16381 tatttgaagc gaaggacaaa aaagatccag agccgaagag ggcgaaacaa tgaaaaagtg
    16441 gctagtgacg attgcagcac tgtggctggc cggatgcagc tccggcgaaa ttaataaaaa
    16501 ctattaccag ttacctgtgg tgcagagcgg tacacaaagt accgccagcc agggcaatcg
    16561 tctgttatgg gtagagcagg tcactgttcc tgactatctg gcggggaatg gtgtggttta
    16621 tcaaaccagt gatgtgaagt atgtgattgc caacaacaac ttgtgggcca gcccgttgga
    16681 tcaacagttg cgcaacaccc tggttgccaa cctgagtacg caactgcccg gctgggtggt
    16741 tgcctcccag cctctgggaa gcgcccagga cacgctcaat gttaccgtaa cggagtttaa
    16801 cggtcgctat gatggcaagg tcattgtcag tggtgagtgg ctgttgaacc accagggaca
    16861 actgatcaaa cgtccgttcc gtctggaagg agtgcaaact caggatggtt acgatgagat
    16921 ggttaaagtg ctggccggtg tctggagtca ggaagccgct tctattgcac aagagataaa
    16981 gcgtctacct taattataaa gatttgtaaa tataaccgtc tccggtatgt tgcctgaggc
    17041 ggtttttttg tttctaacgt gcggaaaaat ttgttcctct tcacattttt tgtacaaccg
    17101 acatgcccgt gtagctcaca aatatgacag tggcgtgaat tttgcgcatt gacggcagtt
    17161 atgattcgcg gtattgctta actgtgattg cacatttagt aatcactgtt ttcttttcca
    17221 ccagaaacca gtatgaggga aacgaggcat gaagagacaa aaacgagatc gcctggaacg
    17281 ggcacatcaa cgtggttatc aggccggcat cgccggacgc tcaaaagaaa tgtgtcccta
    17341 tcagacgctg aatcaaaggt cacaatggct gggaggctgg cgagaagcca tggcggacag
    17401 ggtagtaatg gcctgattct gtctctttaa aaagaaacct ccgcattgcg gaggtttcgc
    17461 cttttgatac tctgtctgat tataatcaga aggcagacgt atcctggaac agaccgactt
    17521 tcaggtcgct ggcggtatag ataagacgac catcaaccag cacttcgcca tccgccaggc
    17581 ccataatcag acgacggtta acaatgcgtt taaagtgaat acggtaggtc acttttttcg
    17641 ctgtcggcag tacctgacca gtgaatttca cttcgccaac gcccagcgcg cggcctttac
    17701 cttcgccgcc cagccagccg aggtagaacc ctaccagctg ccacattgcg tccaggccca
    17761 agcatcccgg cataaccgga tcgccaataa agtggcatcc gaagaaccac agatccggat
    17821 tgatatccag ttctgcttca acataccctt tgtcgaagtt accacccgtt tcggtcattt
    17881 tgaccacacg gtccatcatc agcatgttcg gtgctggcaa ttgcgggcct ttagcgccaa
    17941 acagttcacc gcgaccagag gcaagaaggt cttcttttgt ataggattcg cgtttatcta
    18001 ccatgttctc tgtaagcctt attttattga agcacgcagg atagctaaca cgtgtacgct
    18061 gaacaagtcc gatcagttcg gaataaacca gttcagccaa cgtaatggcc atggaaaacg
    18121 gtgacgtcct tcctgttgcg atgcttgcgc gatacgttcc tggatggttt gcatcagcgt
    18181 cgtttggcct tcgccatccc acaccagatt taataataac ggcagtgcgt cagtcacatc
    18241 gtctactgcc cagatggtga atttgccttc ttctaccgct ttcaccagtt cactgtgaag
    18301 acttaaatgg cgaacgttag ctgtggggat aatgacacct tgtttcccgg ttaactcacg
    18361 ttgctggcaa atagcaaaga agccttcgat tttctcattt aaaccaccga ccggctgggc
    18421 gcgaccgaac tgatcgactg aacctgtgat agcgatactc tgattcaccg gcacatcggc
    18481 gagggcgctt atcagggcgc agagttcagc catcgaggca ctatctccat caacttcact
    18541 gtatgactgc tcaaatgtca gcgatgctga gaaggggatc tgttgctcaa gctgtagttc
    18601 cgacatcagg aacgcttgca tgatcatcat ccctttcgca tggatattgc cgccaagctc
    18661 cgctttgcgt tcgatgtcgg tgaattcacc atcgccaata tgcacaacgc agctaatgcg
    18721 agaaggttcg ccaaaagcgc gtggatgacc cggaaattca atgaccgaaa gggcgttaat
    18781 ttgcccgatg cgttcgcctt cggtttcaat caggatttgc tcctgaagga tctcatcctg
    18841 catacgttca gcgaggaaac cttcgcgcca ttcacgctgc tgcagcatta agtttagctg
    18901 ctcgccggag aaggtgtcgc catcacacag ggaggcgacc tctttacact ggcggaggat
    18961 ccactgcggg ctaagcggaa gtgtttcttg ttcaccggtg tagcgtgctg cttcgcggat
    19021 aagtaccggc caggcatccg ctcccggtgc aggtaagtga ttatgtctgg cggtaaatgt
    19081 cacccagcga caccactggg ttactgactc tgcatcgaca atctgcagag tatcttcaaa
    19141 ttcgctataa atagcctgct ctgaaagctc tggctccatc tcctggaaat cagccaatga
    19201 ttcgcgttcg cctaccagaa tgactttcag cttcaatggc atcgaaggca cagagacggg
    19261 gagagggcgc gactcatcaa acgcaaccca gtcaaaacgc tcgcggttaa cgatattttt
    19321 cagccgcatc cacagcagag gttgcgccag cagtgtacgc aaagagatga tgagaatacc
    19381 gccatttgcc tgatgcacca gaccaggctg cagggtaatg tcgccattaa actggcgcag
    19441 gcagccaaag agttgctccg cttctaccca gtcggcagcg acaacttgcg ttaaagtcgc
    19501 aaaattatca tctgcactca ctgcgtgacg taagcggatg gagtggccgg aaacctcata
    19561 gtgaccgccc accagttggc ctgcatcgct ttgtagcgta cgcgcggcat tggcaataag
    19621 attcagatac tcagactctt ccggggcctt cgccagcata aaagaggagg atgctcgcgt
    19681 atgcagcaac tgctccagcg caaattgcag ccgcggttga gtatcactga ataaaggatc
    19741 gttttcgtca atcaaatgtg gctgagcaaa tatttcctga tagctatcgg tatcaggaac
    19801 caggtcacgc catgcaagtt tcgtaatggt caaagttgat gttttttagt ctgttgtcaa
    19861 agccgcgatt ataccgtaac cggcactaca gcacacgtag aaagcaccga caatactcct
    19921 ggcatgggcg ttaaagctca caggatggag attcttttct tcactggcct aaaaagctga
    19981 tattctgtaa agagttacac ggtaacattg agatcgctat gaaatatcaa caacttgaaa
    20041 atcttgaaag cggttggaaa tggaagtatc tggtgaagaa gcatcgcgaa ggggagttaa
    20101 tcacccgtta catagaagcc agtgccgccc aggaagccgt cgatgtgttg ctctcgctgg
    20161 aaaatgaacc cgtgctggta aatggctgga ttgacaagca tatgaatccg gaactggtca
    20221 atcgcatgaa gcaaaccatt cgggccagac ggaagcgcca ttttaatgcg gaacaccagc
    20281 atacgcgcaa aaaatcgatc gatctggaat ttattgtctg gcaacgtctg gctggtcttg
    20341 cgcagcgtcg cggtaaaacg ctttctgaaa cgattgttca gctgattgaa gatgcggaaa
    20401 acaaagagaa atacgcgaat aaaatgtctt ctctgaagca ggatctgcag gcattgctgg
    20461 gtaaggaata actgacgaaa gtcagttcaa tttactaaag gcaaaaaaaa ccccgcagca
    20521 gcggggtttt tctaccagac gagaacttaa gcttgcggct gagttacaac gtctttgata
    20581 cctttaactt cgatctctac gcgacgatcc ggagccaggc agtcgatcag tgcagcacgc
    20641 tgtttcacgt tgtcacaggt gttgccagta accgggttgg attcgcccat accacgtgcg
    20701 gagatcttgt ctgccgggat acctttggag atcaggtaat caacaacaga ctgagcacgg
    20761 cgctcggaca gaccctggtt gtaagcgtca gaaccgatgc ggtcggtgta acccagaaca
    20821 actacggaac cgtctttcgg atccaggttg ctcagctggc tgtacagctg atccagagca
    20881 gcctgacctt ccggtttcag ggttgctttg ttgaagttga acagaacgtc agacttcaga
    20941 gtgaagtgct tggtctgtac ttccggtgcc ggagctggag ccggagcaac tactggagct
    21001 gcttcgccct gaccgaaacg gtaggaaaca cccaggctca gcatgccgtt gtccggacga
    21061 gtgccgatgg tgtgtgcgtc accgatgttg ttggtccact ggtattccag acgggtagcg
    21121 atttcaggag tgatcgcgta ctcaacacca cctgcgaaaa ccggagaaac gccagtgtcg
    21181 tggtctttaa aggatgcgcc accaggtacg ttagccttgg tgtctgcacg ccataccata
    21241 ccacccagac gagtgtatac gtccagatcg tcagtgattg ggtaacccag tttagcagtc
    21301 agttgaacgc cctgagcttt gtatgcaccg ttgatgttgt cgcctttgta cggcatacga
    21361 cctaaccagt cgtaacccat ttcaaaacca acatacgggt taacctggta accaccaaaa
    21421 gcacctgcac ccagttggtt ttcgtgggtc ggaccattgt taggaataaa accagtgtca
    21481 tggtactggg accagcccag tttagcacca gtgtaccagg tgttatcttt cggagcggcc
    21541 tgcgctacgg tagcgaaacc agccagtgcc actgcaatcg cgatagctgt ctttttcatt
    21601 ttttgcgcct cgttatcatc caaaatacgc catgaatatc tccaacgaga taacacggtt
    21661 aaatccttca ccgggggatc tgctcaatat taactctacc gatatcttcg gcttatgccg
    21721 agcacccctg gcgatgtaaa gtctacaacg tagttgaaaa cttacaagtg tgaactccgt
    21781 caggcatatg aaaaaaaagt cttgtataag gtatgtttaa tcttttttgt cagcgacaat
    21841 ttacagaaga aaatcgcgga aaccgcttca gacaagcctc cgcaaggaaa attagtcacg
    21901 actgaaagca ttggctgggc gacaaaaaaa gttccaggat taatcctaaa tttacttaat
    21961 gatacaaatt agagtgaatt tttagcccgg aaagttgtct cgtggcgtga gaggatgcgc
    22021 ttaccggacg cataataaac cccatagcgt taccttcatt tgccgcatca acaagttcag
    22081 catgctcttc ttcagtcaaa tcatctgcca accaaccgat caccacactg taattgcccg
    22141 tgcgtaaagc gcgaaccatt gactccacag tgtggcaagg ggagagctgg ctaatctgca
    22201 ttactttcgt taagggtagc ccagatgcct gaacccattc ccgactcagt ttttgttgcg
    22261 gtgttaacca gagttgccag cgcgattgct gaccgagttg ctgtaacaat ggcaacagta
    22321 gaagttgcgt catcatgggc tgatcttcgc gatagacaac ttcactgata agcccggctg
    22381 tagtgttttc cgtagagaca cgcgcaattt tacttgctgc ggatgagaac gacgaagaac
    22441 gatgtgcata gcctgaagtg tacataatca atccagcccc tgtgagttac tgtatggata
    22501 tacagtacat ccagtgacaa caaagatcaa ccctattttc ggaaagagcc tcgcaaattt
    22561 tgtcgttggt gacgggaaaa cataaattaa tcttgcccct taagaataag ttgcctattt
    22621 tcgtagttaa cggatccgtt aatgtgaatc attcttttat gttatgattt taaaaggaat
    22681 tttatgaaaa gcctctccta taagcggatc tataaatcac aagaatacct ggcaacgttg
    22741 ggcacaattg aataccgatc attgtttggc agttacagcc tgaccgttga cgacacggtg
    22801 tttgcgatgg tttctgatgg tgagttgtat cttcgggctt gtgagcaaag tgcacagtac
    22861 tgtgtaaaac atccgcctgt ctggctgaca tataaaaagt gtggccgatc cgttaccctc
    22921 aattactatc gggttgatga aagtctatgg cgaaatcaac tgaagctggt gcgtctgtcg
    22981 aaatattctc tcgatgcagc gctgaaagag aaaagcacgc gcaatacccg ggaaagactg
    23041 aaagatttgc tcaatatgtc ttttcatctg gaagcgattc tcggggaggt ggggattaag
    23101 gatgtacggg cgttacgtat acttggggca aaaatgtgtt ggttgcgact gcggcagcaa
    23161 aacagtctgg tgacagaaaa gattctgttt atgcttgaag gtgccattat cggcattcat
    23221 gaagctgcgc tcccggtggc acgccgccag gagcttgcag aatgggctga ctctcttacg
    23281 ccgaaacagg agtttcctgc ggaacttgag taatctggcg ttgcagacga ccaatctcag
    23341 gcagtaatgc aatcaataat ccaacttgtt gtacgaccag aggttctttg ctgtctgccc
    23401 gtggttcaag ttgctgcatc cgctgtttca ggctagctaa tgcctcattg acgcgttctt
    23461 catcagcagg ttgatgatgt aacgcgtcat caacatagca aactgcgtca tcaagaaacg
    23521 ccagaatttc aggattagtt aactgctccc ggtgagcacc gagggctgag atatagctgg
    23581 taaacgtatg gttaaggcac agcaaccgaa acgcggcttc gcgaatttgc ggggtaacgt
    23641 tcggctcgct ggacatattt gataccaccg acgccagctc agcatcacgg ttgtgtgcat
    23701 cgcggcgggc aatacgatac gccagacggt tatcacgccc ctgatggtat tgctccagta
    23761 tggcatcgag ataccgacag ttggcctctg tggcgcgttc gagcatgcgc ggcagattgc
    23821 gaaactgcca gtcaggccag atgtagctca ctgccgccca cgcaatggca caaccaatca
    23881 gcgtatcgat tacgcgaggt aacgctactt caaaaccttc acccagtaag ttaaaacaca
    23941 gtagcaccaa aagtgtgatg aacatcgttg catgagcgta ttgcacgtta cggaaggcaa
    24001 aaaagagcac gccggtaata accagcagca ccagctgccc ttccagtgat ggcacaaacc
    24061 acagcacagg aatgccaatg gcgataccta ccagcgtacc aataatcctt aacttcaggc
    24121 ggtggcgcgt ggcgttatag tttggctggc agacaaacaa acttgtcagc aagatccaat
    24181 acccgtgatg cattccggtt atttgaatga tggcgtagcc gaagcacaac accagcgaca
    24241 ttcttaccgc atgacggaag agggcggatt ccggcgtgaa gtgacggcta agacgcagcc
    24301 agatatcact caacccgtgc gggctgtcat cagcgagctc attttcgtca ttattatggg
    24361 gtagtgcctg ggcctgttct gattcaattg ttgccagttg ggcatcaatg gcgcgtaaat
    24421 tgttcagcaa aaatcccagt gttttgagta aatcggcggg tgcgccgtta tcgcgcatcc
    24481 gctccagcgc agcatcaata tgcgtaaaag cgcgctcaaa atgcggatca tgttgataag
    24541 gctgacgcag caaaatacag cgtgacagtt gctggcacgc ctggccctgc atcgacatca
    24601 gccgctgaaa acggaacagc acgtcgctgt ggcgaaaatg ttcacgcaat gtttgatact
    24661 gaatatgaga agagctggca cgctcgtgaa tatcctgcgc gacaaagtaa taatgcagcg
    24721 tgcgacgcgt tccccgttga ccacgatcgc cacgtaagcg ggtcagcagc gagagtttcg
    24781 tctgattcaa tgtcgccatc agttgtccgt tggcgagagc caaatcgtac agcggtgcct
    24841 ggctttcatc ttcaatatca ggatcaaaca tgcgcgactt gagctcaaga taacgcgcca
    24901 gttgttcata gcaacgcgcc aggttgtcct gcagcgggcg gaccgggaac agcagatgac
    24961 caataagtgt caggacgttg taccagacgg caccggccag cagatacatc ggctgctgat
    25021 accagtgctc atacagtgat gttcccaaca tagtgtaaat ggcgatcagc aatgcaccga
    25081 aggcaattgt tgcatagcgt tgacccagac cgccgagcaa aatgaagccg ctggtagaga
    25141 gcgttaagcc aatcgcaaat agccagggcc agggaaacag caattctact gaggccgagg
    25201 cgataaaaaa gcagaacagc gtaatgatga ggttacgcaa acgtcccgcc agtcggtcat
    25261 cgagatcggt cagcgccgct gccaccatcc ccagcgttag cggaatcgtc agttttacat
    25321 cacccagcca ccacggaaac gctgtggttc cacaaagcgc aataaaaata cgcgcgtaat
    25381 acagccaggc gctgttccag gtatagcgtt tgagcaaagg acttagcata aaggccatat
    25441 tagtggatta ttcaaaacga cgacgtgcat tggcttcacg cgcagcttgc gctgtttcta
    25501 ccgataccac gcgacgacca accggccata gtgcaatagc ggcaatttta aagttcgcaa
    25561 tgccaacagg aatgccaatg attgaaatac attgtgcgat gcccgttgca atgtgcatca
    25621 ggcataacca ccagccaaag aaaatcagcc agaaaatatt caataccgta ccgccagtat
    25681 tcagcagcac atttttgcca gccgggttca gttcatcgac atgaatagct tcattgccat
    25741 aaggcaccag agacagttta gtgatctccc agcaggatcg tgtcagcggt aaggtaaaaa
    25801 tcagcacaat actgaccaga gtcgccaaca gccagcccag agtggtggca aatccgccaa
    25861 gcacaaagtt cagaatgttc aaaacggtac gcataaaacc tcgctttact gtggttttca
    25921 gtaatgggcg gcaattgtaa cgtttttttg ggctggagca cgttttctct gacggttaca
    25981 ctgataagaa ataatttcgt gtggatctac agagtcatgg aactgaaagc gacaacgctt
    26041 ggaaaacgtc tggcacagca cccttacgat cgggcggtga tcctcaatgc cgggattaaa
    26101 gtctccggcg atcgccacga ataccttatt cctttcaatc aattactggc gattcactgt
    26161 aagcgcggtc tggtatgggg cgagctggaa tttgtactgc cggacgaaaa agtggtgcgt
    26221 ctgcacggca ccgaatgggg cgagacgcag cgtttttacc atcatcttga tgctcactgg
    26281 cggcggtgga gtggcgagat gagcgaaatt gcgtctggtg ttttacgcca gcaactggat
    26341 ttgattgcca cgcgcactgg agaaaataaa tggctgacgc gtgagcaaac ctctggcgtt
    26401 cagcaacaaa tccgccaggc tttgtcggcg ttgccgttgc cggttaaccg actggaagaa
    26461 ttcgataact gccgtgaggc gtggcgtaaa tgtcaggcct ggttgaaaga tattgaaagc
    26521 gctcggttgc agcataacca ggcgtatacc gaagccatgc ttaccgagta tgcggatttt
    26581 ttccgccagg tcgagtcttc accgctgaat ccggcgcagg cccgggcagt cgttaatggc
    26641 gagcattctc tgttagtgct ggcaggtgca ggaagcggaa aaacgtcggt gctggtggcc
    26701 cgtgcaggct ggttgctggc gcgtggtgaa gcgtcccctg agcaaatttt attgctggcg
    26761 tttggtcgca aagccgctga agagatggac gagcggattc gcgaacggct acataccgaa
    26821 gacattaccg cacgcacgtt tcatgcgctg gcgctgcata ttattcagca gggcagcaaa
    26881 aaagttccga tagtcagcaa actggaaaat gataccgctg cccgtcatga actctttatt
    26941 gctgagtggc gcaagcaatg cagcgaaaag aaagcgcagg cgaagggctg gcggcaatgg
    27001 ctgacggaag aaatgcagtg gtcagtgcca gaaggtaact tctgggatga tgaaaaatta
    27061 cagcgtcgcc ttgcctctcg cctcgatctc tgggtaagtc tgatgcggat gcacggtggt
    27121 gcacaggcag aaatgattgc cagtgcaccc gaagagattc gcgatctgtt cagtaaacgt
    27181 atcaagttga tggccccgtt attaaaagcc tggaaaggtg cgctgaaggc agaaaacgct
    27241 gtcgattttt cgggccttat tcatcaggcg attgtgattc tggagaaagg tcgctttatc
    27301 agcccgtgga agcatattct ggttgatgaa tttcaggata tctcgccgca gcgggcagcg
    27361 ttgttagcgg cattacgcaa gcaaaacagt cagacgacgt tgttcgctgt tggtgatgac
    27421 tggcaggcga tttaccgatt cagcggtgcg caaatgtcgc tcaccaccgc tttccatgaa
    27481 aactttggtg aaggcgaacg ttgtgattta gacacgactt accgttttaa cagtcgtatc
    27541 ggtaaggtgg caaaccggtt tattcagcag aacccaggcc agctgaaaaa gccgctaaac
    27601 agcttaacca atggagacaa aaaagccgtc acgttattgg atgagagtca acttgacgct
    27661 ttgctggata agctctctgg ttatgccaaa ccggaagagc gcattctgat cctggcgcgt
    27721 taccatcaca tgaggcctgc cagcctggaa aaagcggcaa cacgctggcc gaagttgcaa
    27781 atcgacttta tgaccattca tgccagcaaa gggcaacagg cggattacgt catcatcgtt
    27841 ggcttgcagg agggaagtga tggttttccg gctgcggcgc gggaatcgat tatggaagag
    27901 gcgctactgc caccggttga ggatttcccg gacgcggaag aacggcggtt aatgtacgtg
    27961 gcgctgaccc gggcacgcca tcgggtatgg gcactgttta acaaagagaa tccctctccc
    28021 tttgtggaaa tactgaaaaa tctggatgtg ccggtggcga gaaaaccgta agaaacaggt
    28081 ggcgtttgcc acctgtgcaa tattacttca gacggtccgc gagataacgc tgataatcgg
    28141 ggatcagaat atcgaccgcg tcgttgaaat gcggcgactg gattatgaag tctgccgttg
    28201 ccacgttggt ggcgaccgga atgttccata ccgtcgccag acgcagcaag gctttcacgt
    28261 caggatcgtg cggcacggca tttagtggat cccagaagaa aatcaataca tcaattttcc
    28321 cttctgagat caatgcgcca acctgctggt caccccccat tgggccactc aacatcgcgt
    28381 tgacgttcat gccggtcgcg cgggaaatta agttaccggt agtgcctgtt gcatacagta
    28441 cgtgttgttc cagtaacggt tgatgccgtt ccacccagct catcagcatt tgtttgcagt
    28501 gatcgtgtgc caccagcgca atatgtttcc gcgcaggtaa agtgcgagtc gtcagttcca
    28561 taatgtacat ccgtagttaa ctttcctaca gattactgta agcacttatc gctgcaagat
    28621 aaagaccgaa aaagcctgcg cacaggcaca aaaatctcag gaagatggtt gtttttccgc
    28681 ccattgcagg aaagtatttc gcgtttgtgg gtcagccagt ttaaaccaat acttcagccg
    28741 ttgttctgtg agcacctgag actgcggtgg aatagcatcc agctcggaaa cacccgaaag
    28801 cagcgtactg tcatctgcca tcatcgtggc aaattgcggt agtgaagcgc gtttcgcgga
    28861 tttgttatag cgttcaacct cgacctcata atcaatcgtt tttgccgttg aggtaatggc
    28921 gagaatatcc agctttaccg gaatcggcgt cgcatcgcca tccaacaatt caaggcgcgg
    28981 cgcggcatca aaatggttag cttcccgctc attctccagg cgaggcaggc gaaaattgac
    29041 ctggttgatg agctgggtat taaaactgac caccagcggt ggggagatat acagccgttc
    29101 ttcactattg gaaagatgaa tcgtcttctc aacgcgaaac actaactgat gcggtccatt
    29161 atccagttca atgctgtccg caccgcgcag cagagagctg gaaaccttct tgccgtcgag
    29221 taccagaaga tcgacatcgg ttgaaagccg cagcgtggtg gcaaaaacgg ataccggcaa
    29281 acataatgca atcaaggtgg tcacgatgcc ggttttcata ggtagctcct gtcaaaagac
    29341 cgcccgaata ataatgtgtc aaaatttttc tttaatcata cttactaaca tatagacata
    29401 tttcccgctt ttgctctcaa tcattcgtat tagctgcatg gttggcatgt cggctttgtc
    29461 gtacactttg ttaaacagtc tggaggaaat aatgaaagaa accgatattg caggtatttt
    29521 aacgtccact cacaccattg cgctggtggg ggcaagcgac aaacccgatc gtcccagcta
    29581 tcgcgtgatg aaatatctac tcgaccaggg gtatcacgtc attccggttt cgccaaaagt
    29641 tgccggcaaa acgctgctgg gccagcaggg gtatggcacg ctggcagacg ttccggaaaa
    29701 agtcgatatg gtcgatgttt tccgtaattc agaagcggcg tggggcgtgg cgcaggaagc
    29761 tatcgccatt ggcgcgaaaa ccctctggat gcaactgggg gtgattaatg agcaagcggc
    29821 agtactggca cgggatgccg ggttaaatgt ggtgatggat cgctgcccgg ctattgagat
    29881 ccctcgcctg ggcctggcca aataaaaaat ccccggaagg caaaaacctt ccggggattc
    29941 gttcagggat cagttacgca gacgcggggc ctggagttgt ttgcggatgg tctgcgccag
    30001 ctcatccatg gatggctgtt caggatgttc atcctgcaat tcgctactta gctgggcttc
    30061 agccaggtag gtatgaaccg gtaggccgtt atcgtcctcc atcaccacgt gataccacgg
    30121 agcggcgcga agctcgtcat tcaccgccag ctcatcaggc gacggttcag agagcgaata
    30181 aaccgggtcg atatccacga ccactccgag ataacctaac agggaatggc ggacctgctg
    30241 gccgataccg aatttgctgg caatcatagt cacctcccgg gaaatcttta cctttgatat
    30301 aagggtaaaa ttccacattt caagttacat gacgcgacag gcaaaccctt tcagatatag
    30361 cccttccgga taggtagcga tcaccggatg atcggctgcc tgacggaact gctctataaa
    30421 ttgtacatca cggccggcat caattgcggc atccgcgatg attttctgaa ataaatcgct
    30481 ggtcatcaga ccggaacagg agaaagtcag gagaataccg ccttcattca gcaactgaat
    30541 cgccagcatg ttgatatctt tatagccacg acacgcgccc atcaactggc ttttattctc
    30601 aacaaacttc ggcgggtcca tcacgataac gtcaaatttt tcaccgcgat cgcgataagt
    30661 acgcagcaat ttaaagacat catcacggac aaactcagcc ttgctcagat ccagtttgtt
    30721 cagctcaacg ttctgccgtg caatatccag cgcttcctgg gaggtatcaa cgctgacaac
    30781 ctggctgcaa ccgcccatca gtgccgatac ggcgaaacca ccggtatagg agaaacagtt
    30841 cagcacacgt ttattttcaa cgtagcggcg ggtagccagg cggctgtctc gctggtccag
    30901 gtagtagccc gttttgtgtc cgtgctgaat atccaccagc agtttcatgc cgtgttcttc
    30961 aatcggcagc agggcaggtg gcaactcgcc ggtgacgagg ccctgggtca gctccatccc
    31021 ttctttttta cgtaccgcaa catcgctgcg atcgtaaatc gcacattccg ggtacagcgt
    31081 ttgcagggca ctaattaatg ccgcgcgctg atattctgcg ccagcactca gcagttgcag
    31141 caccagaaaa ttaccgaaac gatcgatagt aatacccggc aggccatcag attctccggc
    31201 gattaaacga tagctgtcga ggccatcttt ttgcgccagc cagtcacgcc atttttgtgc
    31261 ttgttgcaaa cggcgggaaa aaaaagcaat gtcgatagac tcagacgggt caaacgtcca
    31321 gacgcgcgcc cggatttgcg aagctggcga ataagcgccg cgtgctaacc attttccctg
    31381 atgatcaaca atatcgatgg tttcaccgag gctggcttta ccttccatgc gggcaacggc
    31441 cccggaaaag acccacggat ggcgacgaag taatgatttt tcgcgccctt tggctaacac
    31501 taaacgtaca ctcataattt acttttctgt cgatgcaaag aaatgggcgt cattgtccgg
    31561 agttttccat ggatttgcaa cgcgtccatt aaggataagg agagcagcaa tgtcgaaagt
    31621 ctgcataatt gcctgggttt acgggcgagt tcagggcgta ggatttcgct acaccacaca
    31681 gtatgaagcg aaaagactgg gtttaaccgg gtacgccaaa aatcttgacg atggcagcgt
    31741 ggaagtggtt gcctgcggtg aagaagggca ggtggaaaaa ttaatgcagt ggttaaagag
    31801 tggcggcccg cgttctgcgc gtgtggaacg ggtgcttagc gagccgcatc atccctcggg
    31861 ggaattagcc gattttcgta tccgttaaat acattttacc ggttttggca ggccagcaat
    31921 tttggtggct tgcttagccg gacctttcgg aaacagtcga tataaatagc ggctgttacc
    31981 tttttcttcg ccaaatttat tcgccatcgc ttttaccagc atacgaatcg ccggagaagt
    32041 attgaattcc agatagaaat cacgcacaaa acgcaccact tcccagtgtt ctggcgacag
    32101 cgaaatccct tcgttctctg caatcaccac agccagcggc tcactccact ggctgctttc
    32161 tttgagatag ccttcggtat ccgtttctat ctctttacct tcgaagatca gcataattat
    32221 tactacctta atcagactgg cggcagtgta acaaaaaaca aagccccgca taaagcgagg
    32281 ctatgaaagt gttagcgggt gagattaatc gcggctagcg aagcccagaa tgctcagcag
    32341 gctgacgaag atgttgtaca gcgaaacata caggctaacc gtggcacgaa tatagttcgt
    32401 ctcaccgcca tgaatgatgt tgctggtttc aaacaagata gcgccagagg agatcagaat
    32461 gaagaccgcg ctgatcgcca gatgcagagc aggcagttgc aggaagatat tagcaaccat
    32521 accaatcagc accaccacaa tacccgccat cagcataccg ccgaggaacg acatatcttt
    32581 gcgggtggtc agcacatatg cagagcagca gaagaacact aacgccgttc cgcccagtgc
    32641 catagcgatt acgtcaccca ttccggcaga cagataggtg ttcagaatag gtccgaggat
    32701 ataacccaga aaaccggtaa aggcgaatgc ggagataatc ccggtcggct tattcgccgt
    32761 tttataggtc aggaacatca aaccatacat acccaccagc gtcagaatca gacccggaga
    32821 tggcagcatc agcaccgtac tggcagttgc ggtaatcgcc gaaaaggcca gcgtcaggct
    32881 cagcagaaaa taggtattac gcagcacctt atgggtgcta agcagtgatg tacggtcatg
    32941 tgaagaacta acaatacgat ccattgagtc actctctatg acagatgtaa ttaattaagc
    33001 agcataatga taatgcgtaa gggcacccag aagttttacc catctttacg catttgatct
    33061 ggaacaggtt taacagcgga ttatcaggtc attaagcaaa tataacgccc tgagaatttc
    33121 gacaggcaaa agaaaaaggg gttagcattt agctaacccc ttatcttatt tggcggaagc
    33181 gcagagattc gaactctgga accctttcgg gtcgccggtt ttcaagaccg gtgccttcaa
    33241 ccgctcggcc acacttccgg aatgacgcgc actataaaca tcccgatgcg gcgtgtaaac
    33301 ccctaatttg tttgtttgcc tgaaaaacag ccaaaagtgc attgatagcg tgaaataaca
    33361 gcagattgat catttcatca ccatgaattc cttctctttt actcgtttag caaccggcta
    33421 aacatcccca ccgcccggcc aaaagaaaaa taggtccatt tttatcgcta aaagataaat
    33481 ccacacagtt tgtattgttt tgtgcaaaag tttcactacg ctttattaac aatactttct
    33541 ggcgacgtgt gccagtgcag aaggatgagc tttcgttttc agcatctcac gtgaagcgat
    33601 ggtttgcctt gctacaggga cgtcgcttgc cgaccataag cgcccggtgt cctgccggtg
    33661 ttgctaggag aagagacgtg cgatatgaat aacgaggaaa cattttacca ggccatgcgg
    33721 cgtcagggcg ttacccggcg cagctttctc aaatattgta gtctggctgc cacgtcgctg
    33781 ggattaggcg cgggaatggc accaaagatt gcctgggcgc tggagaacaa accgcgcatt
    33841 ccggtggtat ggatccacgg tctggaatgc acctgctgta ccgaatcttt tatccgctcc
    33901 gctcacccac tggcgaagga cgtcatcctt tcccttattt ctctcgatta cgacgatact
    33961 ttgatggctg ccgccggaaa ccaggcggaa gaagtctttg aagacatcat cacgcaatac
    34021 aatggcaaat atatcctcgc agtagaaggc aatccgccgc tgggcgagca ggggatgttc
    34081 tgtatcagca gcggtcgacc gtttattgag aaactcaaac gtgccgctgc cggagccagc
    34141 gcgattatcg cctggggaac ctgcgcgtcc tggggctgcg tgcaggccgc gcggcccaat
    34201 ccgacgcagg caacgcctat cgacaaagtc atcaccgaca aacccattat caaagtacct
    34261 ggctgcccgc cgatcccgga tgtgatgagc gccatcatta cttacatggt gacctttgat
    34321 cgcttgccag atgtcgacag aatgggccgt ccactgatgt tttatggtca gcgtatccat
    34381 gataaatgct atcgccgcgc tcacttcgac gccggagagt tcgtccagag ttgggatgat
    34441 gacgctgccc gcaaaggtta ctgcctgtac aaaatgggct gcaaagggcc taccacctat
    34501 aacgcctgtt cctccacacg ctggaatgat ggcgtttctt ttccaatcca gtctggtcac
    34561 ggctgcctgg gctgtgcgga aaatggtttc tgggatcgcg gttcgttcta cagccgcgtg
    34621 gtcgatattc cgcaaatggg tactcattcc actgccgata ccgtcggttt aaccgcgctt
    34681 ggcgtggtgg cagcggctgt tggtgtgcac gcagtcgcca gcgccgttga ccagcgcaga
    34741 cgtcataacc agcaacctac agaaaccgaa catcagccag gcaatgagga taaacaggca
    34801 tgagcactca gtacgaaact cagggataca ccatcaataa tgccggacgc cgcctggtgg
    34861 tcgacccgat tacgcgcatc gaaggccaca tgcgctgcga agtgaatatt aacgatcaga
    34921 atgtgatcac caatgccgtc tcctgcggca ccatgtttcg cgggctggag atcattctgc
    34981 aagggcgcga cccgcgcgat gcgtgggcgt tcgttgaacg tatctgcggc gtctgtactg
    35041 gcgtacacgc cctggcttcg gtttacgcca tcgaagatgc tatcggtatt aaagtgccgg
    35101 acaacgccaa tatcatccgc aacattatgc tggcaacgct ctggtgccac gatcatctgg
    35161 tgcacttcta tcagcttgcc gggatggact ggatcgatgt gttagatgcg ctgaaagccg
    35221 acccgcggaa aacctccgaa ctggcgcaaa gtctctcctc ttggccgaaa tcatcccctg
    35281 gctatttctt cgacgtacaa aaccgcctga aaaaatttgt tgaaggcggg cagttgggga
    35341 tcttccgcaa tggctactgg gggcacccgc agtacaaact gccgccagaa gctaacctga
    35401 tgggctttgc ccactatctc gaagctctcg atttccagcg tgaaattgtc aaaatccacg
    35461 cggtctttgg cggtaaaaac ccgcatccaa actggattgt cggcgggatg ccttgcgcca
    35521 tcaacattga cgaaagcggc gcggtcgggg cagtcaatat ggaacgcctg aacctggtgc
    35581 agtcaattat cacccgcacg gcggacttca ttaacaacgt gatgatcccc gacgccttag
    35641 ccatcggtca gttcaacaaa ccgtggagcg aaatcggcac gggtctttct gataaatgtg
    35701 tcctcagcta cggggcattc ccggatattg ccaacgactt tggtgagaaa agtctgctga
    35761 tgcctggcgg cgcggtgatt aacggcgatt tcaacaatgt gctgccagtg gatttggttg
    35821 atccgcagca ggtgcaggag tttgtcgatc acgcctggta tcgttatccc aacgatcagg
    35881 tcgggcgtca tccgtttgat ggcatcaccg acccgtggta caaccccggc gatgtcaaag
    35941 gcagcgatac caacattcag cagctgaatg aacaggaacg ctactcgtgg atcaaagcgc
    36001 cacgctggcg cggtaacgcg atggaagtgg ggccgctggc gcgcacgtta atcgcttatc
    36061 acaaaggcga tgctgcgacc gttgagtcgg tcgatcgcat gatgtcggcg ttgaacctgc
    36121 cgctttccgg tatccagtca acgttaggcc gcattttgtg ccgcgcgcac gaagcgcagt
    36181 gggccgcagg taagttgcag tatttcttcg acaagctgat gaccaacctg aaaaacggca
    36241 atctcgccac tgcttccacg gaaaaatggg aacctgcaac ctggccgaca gagtgccgtg
    36301 gtgtcggttt taccgaagcg ccgcgcgggg cgttaggcca ctgggccgcc attcgcgatg
    36361 gcaagattga tctctaccag tgcgtggtgc cgaccacctg gaacgccagc ccgcgcgatc
    36421 ccaaagggca gattggcgct tatgaagcgg cgctgatgaa caccaaaatg gcgatccccg
    36481 agcaaccgct ggagatcctg cgtactctgc acagctttga cccgtgcctc gcctgttcaa
    36541 cacacgtgct gggcgacgac ggtagcgagc tgatctccgt gcaggtgcgt taacagcgaa
    36601 ggagaatcat catgcaacag aaaagcgaca acgttgtcag ccactatgtc tttgaagcgc
    36661 cagtccgcat ctggcactgg ttgacggtgt tatgcatggc ggtgttgatg gtcaccggat
    36721 actttatcgg caagccgcta ccttccgtca gcggcgaggc gacgtatctg ttctatatgg
    36781 gctacatcag gttaattcac ttcagcgccg ggatggtttt taccgtggtt ttgctgatgc
    36841 ggatctactg ggcttttgtt ggcaatcgat actcccgcga gctgtttatc gtgccggtat
    36901 ggcgtaaaag ctggtggcag ggcgtgtggt atgaaatccg ctggtatctg tttctggcaa
    36961 aacgtccgag tgccgatata ggccataatc ccatcgccca ggcggcgatg ttcggctatt
    37021 tcctgatgtc ggtctttatg atcatcactg gttttgcgct gtacagcgaa cacagccagt
    37081 acgctatttt tgcgccgttc cgttatgtgg tggaattttt ctactggacg ggtggcaact
    37141 caatggacat tcacagctgg catcggctgg ggatgtggct gattggcgcg tttgtgatcg
    37201 gtcatgtcta catggcgctg cgtgaagaca tcatgtccga cgacacggtg atctccacca
    37261 tggtcaacgg ctaccgtagc cacaaatttg gcaaaataag taacaaggag cgttcatgag
    37321 cgagcaacgc gtggtggtca tggggctggg caacctgctg tgggccgatg aaggcttcgg
    37381 cgtgcgggtg gcggaacggc tgtatgccca ttaccactgg cccgagtatg tggagattgt
    37441 cgatggcggt actcagggac tgaacttgct ggggtatgtc gaaagcgcca gccatctgtt
    37501 gattatcgat gccattgact acgggctgga acctggaacg ctgcgaacct atgccggaga
    37561 acgcattccg gcttatctca gcgcgaagaa aatgagcctg catcagaaca gtttctccga
    37621 agtgttggcg ctggcggata tccgcggaca tctgccagca catattgccc tcgtcggtct
    37681 gcaacccgca atgctcgacg actacggcgg tagcctgagc gaactggcac gggagcaact
    37741 gcccgctgcg gaacaggcgg cgctggcgca gctggctgcg tggggaattg tgccgcaacc
    37801 ggctaatgaa tcgcgctgtc tcaattatga ctgtctgtcg atggaaaatt acgaaggcgt
    37861 tcgcttgcgc cagtaccgga tgacacagga ggagcaggga tgagcaacga cacgccattt
    37921 gatgcgttgt ggcaacgaat gctggcgcgc ggctggacgc cagtcagtga atcccgtctt
    37981 gacgactggc ttacgcaagc gccagacggc gtggtgttat taagcagtga cccgaaacgc
    38041 acgccagagg tcagcgataa tccggtaatg attggcgaat tactgcacga gtttcccgac
    38101 tatacatggc aggtggcgat tgctgacctt gagcagagcg aagccatcgg cgatcgcttt
    38161 ggcgtctttc gctttcctgc cactttagtg tttaccggcg gaaactatcg cggcgtgctg
    38221 aatggtattc acccgtgggc ggaactgata aacctgatgc gcgggcttgt cgaaccgcag
    38281 caggagcgtg cctcatgagc gaaacttttt tccatctgct ggggccagga acgcaaccga
    38341 acgatgacag tttcagcatg aatccactgc cgatcacctg tcaggtgaat gatgaaccga
    38401 gtatggcggc cctggagcaa tgtgctcaca gcccgcaggt gattgcgctg ttaaacgagt
    38461 tacaacatca actaagcgaa cgccaaccgc cgttgggcga ggtgctggca gtcgatctgt
    38521 taaatctcaa cgccgacgat cgtcacttta tcaatacgct tctcggggaa ggggaagtgt
    38581 cagtgcgcat tcagcaggct gacgacagtg aaagtgaaat acaggaggcg atcttctgcg
    38641 gattatggcg ggtgcgcaga cgtcgcggcg aaaagttgct ggaggacaaa ctggaggctg
    38701 gctgcgcgcc gctggcgttg tggcaggcgg caacgcaaaa tctcttgccg acagattcgc
    38761 tgttaccgcc gcccattgat ggcctgatga atggcctacc gttggcgcat gagttactgg
    38821 cacatgtacg taaccccgac gcgcagccgc acagcattaa tctgacgcaa ttacccatca
    38881 gcgaggctga tcggcttttt ctctcacgtc tctgtgggcc gggaaatatt cagattcgta
    38941 ccattggcta tggcgagagc tatatcaacg ccacggggtt acgccatgtc tggcatttac
    39001 gctgtacgga caccttaaaa ggcccgttac tggaaagtta tgaaatctgc ccaataccgg
    39061 aagtggtgct ggcagcgcca gaagatttgg tcgactctgc gcagcggctt agcgaggtat
    39121 gtcagtggct ggcggaagct gcaccgacgt aaaaagacgg taagtatcgc tttcagtctt
    39181 atgaatatcg caatcggcga atacctctgg tcgtagagtt tcaggataaa gagggagatc
    39241 taccattatc gggttatttt tctctcttcg cctacaggag tgcgcatgtg ggatgtcatt
    39301 gatttatcgc gctggcagtt tgctctgacc gcgctgtatc actttttatt tgtacccctt
    39361 accctggggc tgattttttt gctggctatc atggaaacca tttacgtggt caccggcaaa
    39421 acaatctacc gcgatatgac gcgcttctgg ggtaagctct tcggtatcaa ttttgctctt
    39481 ggcgtggcta ccggcctgac catggagttt cagtttggta ctaactggtc attctattcc
    39541 aactatgtgg gcgatatttt tggcgcacca ctggcgatgg aagcattaat ggccttcttc
    39601 ctcgaatcca cctttgtcgg gctgttcttc ttcggctggc aacggctgaa taaataccag
    39661 cacctgctgg tgacgtggct ggtggcgttc ggttcaaatc tctctgcgtt gtggatattg
    39721 aatgccaatg gttggatgca atacccgacc ggtgcgcatt ttgatatcga caccctgcgt
    39781 atggagatga ccagcttcag cgagctggtc tttaatccgg tcagccaggt gaaatttgtg
    39841 cacaccgtaa tggcgggcta cgtgaccggg gccatgttta ttatggcgat cagcgcctgg
    39901 tatttactgc gcggacggga gcgcgatgtc gcattacgct cgtttgccat cggttccgtc
    39961 ttcggtactc tggcgattat cggtaccctg caactcggag acagttctgc gtatgaagtc
    40021 gcgcaagtac aaccggtaaa actggcggcg atggaagggg agtggcaaac ggaacctgca
    40081 cctgcaccgt tccatgtggt tgcctggccg gaacaggatc aagagcgtaa cgcctttgcc
    40141 ctcaaaattc ccgcgctgct agggatcctc gccactcact cattagataa acccgtgccg
    40201 ggtctgaaga atttgatggc tgaaacctac ccacgcttgc aacgcggacg tatggcctgg
    40261 ctgttaatgc aggaaatatc gcaaggcaat cgtgagccgc atgtgttgca ggcattccgg
    40321 gaactggaag gtgacctggg ctacggcatg ttgctctccc gctatgcgcc ggatatgaat
    40381 catgtcacag ccgcacagta ccaggcggcg atgcgtggcg cgatacctca ggttgcgccg
    40441 gtattctgga gtttccgcat catggtgggc tgtggttccc tgctgctact ggtgatgctg
    40501 attgcgcttg tccagacgct gcgtggcaaa atcgaccagc atcgctgggt gctgaaaatg
    40561 gcgctctgga gtttgccgtt gccgtggatt gcgattgaag ccgggtggtt tatgaccgag
    40621 tttggtcgtc agccgtgggc gatacaggac atcttaccga catactccgc gcactccgct
    40681 ttaaccacag gacaactggc tttctcactg atcatgatcg tagggcttta caccctgttc
    40741 ttaatcgccg aagtctacct gatgcagaaa tatgcccgtc tggggccgag cgcgatgcag
    40801 agtgaacaac cgacgcagca acaggggtaa aggagaaaat catgtttgat tatgaaacat
    40861 tgcgcttcat ctggtggctg ctgattggcg tgatcctggt ggtctttatg atctccgacg
    40921 gatttgacat ggggattggc tgtttgctgc cgctggtggc gcgtgatgat gatgaacgcc
    40981 ggatagtgat taatagcgtt ggtgcgcact gggaaggcaa tcaggtctgg ttgatcctcg
    41041 caggtggggc attatttgcc gcctggccca gagtgtatgc agcggcgttt tccggctttt
    41101 atgtggcgat gatcctggtg ctgtgctcgc tgttcttccg cccgctggcc tttgattatc
    41161 gcggaaaaat cgccgatgca cgctggcgta aaatgtggga tgccggtctg gtcatcggca
    41221 gtctggtgcc gccggtagtc ttcggtatcg ccttcggcaa cttgttgctc ggcgtgccgt
    41281 ttgccttcac accgcaatta cgcgtggagt atctcggcag cttctggcaa ctgctgacgc
    41341 cattcccttt attgtgcgga ttgctcagcc ttgggatggt gattttgcaa ggtggcgtct
    41401 ggttacaact gaaaactgtt ggtgtgattc atctgcgttc acagctggcg actaaacgcg
    41461 ctgcactgtt ggtgatgctg tgctttttgc tggcgggtta ctggctgtgg gtcggtattg
    41521 atggctttgt actgctcgcc caggatgcta acggtccttc caatccgtta atgaaactgg
    41581 tggcagtgct acctggtgcc tggatgaata attttgtcga gtcgcccgtt ttgtggatct
    41641 tcccgctgct ggggttcttc tgcccattgc tgacggtgat ggcgatttat cgtggtcgcc
    41701 cgggttgggg atttttgatg gcgtcattga tgcaatttgg cgtgattttc acggcaggca
    41761 tcacgctgtt cccctttgtc atgccgtcaa gcgtgagtcc aatctccagc ctgacgttgt
    41821 gggacagcac ttccagtcag ctgacgctga gcattatgtt ggtaatcgtg ctgatatttt
    41881 tgcctattgt gttgctctac actctctgga gctactacaa aatgtgggga cgcatgacga
    41941 cagaaactct ccgccgtaac gaaaacgagc tgtactaagg aacaggaaca atgtggtatt
    42001 tactttggtt cgtcggcatt ttgttgatgt gttcgctctc cacccttgtg ttggtatggc
    42061 tggacccgcg tctgaaaagt taacgaacgt aggcctgatg cggcgcatta gcatcgcatc
    42121 aggcaatcaa taatgtcaga tatgaaaagc ggaaacatat cgatgaaagc gatcttaatc
    42181 ccatttttat ctcttctgat tccgttaacc ccgcaatctg cattcgctca gagtgagccg
    42241 gagctgaagc tggaaagtgt ggtgattgtc agtcgtcatg gtgtgcgtgc tccaaccaag
    42301 gccacgcaac tgatgcagga tgtcacccca gacgcatggc caacctggcc ggtaaaactg
    42361 ggttggctga caccgcgcgg tggtgagcta atcgcctatc tcggacatta ccaacgccag
    42421 cgtctggtag ccgacggatt gctggcgaaa aagggctgcc cgcagtctgg tcaggtcgcg
    42481 attattgctg atgtcgacga gcgtacccgt aaaacaggcg aagccttcgc cgccgggctg
    42541 gcacctgact gtgcaataac cgtacatacc caggcagata cgtccagtcc cgatccgtta
    42601 tttaatcctc taaaaactgg cgtttgccaa ctggataacg cgaacgtgac tgacgcgatc
    42661 ctcagcaggg caggaggggc aattgctgac tttaccgggc atcggcaaac ggcgtttcgc
    42721 gaactggaac gggtgcttaa ttttccgcaa tcaaacttgt gccttaaacg tgagaaacag
    42781 gacgaaagct gttcattaac gcaggcatta ccatcggaac tcaagatgag cgccgacaat
    42841 gtctcattaa ccggtgcggt aagcctcgca tcaatgctga cggagatatt tctcctgcaa
    42901 caagcacagg gaatgccgga gccggggtgg ggaaggatca ccgattcaca ccagtggaac
    42961 accttgctaa gtttgcataa cgcgcaattt tatttgctac aacgcacgcc agaggttgcc
    43021 cgcagccgcg ccaccccgtt attagatttg atcaagacag cgttgacgcc ccatccaccg
    43081 caaaaacagg cgtatggtgt gacattaccc acttcagtgc tgtttatcgc cggacacgat
    43141 actaatctgg caaatctcgg cggcgcactg gagctcaact ggacgcttcc cggtcagccg
    43201 gataacacgc cgccaggtgg tgaactggtg tttgaacgct ggcgtcggct tagcgataac
    43261 agccagtgga ttcaggtttc gctggtcttc cagactttac agcagatgcg tgataaaacg
    43321 ccgctatcat taaatacgcc gcccggagag gtgaaactga ccctggcagg atgtgaagag
    43381 cgaaatgcgc agggcatgtg ttcgttggca ggttttacgc aaatcgtgaa tgaagcacgc
    43441 ataccggcgt gcagtttgta atgcataaaa aagagcattc agttacctga atgctctgag
    43501 gctgatgaca aacgaagaac tgtttaatgc gtagaccgga agaggcgttc acgccgcatc
    43561 cggtcacttt accttcagtt ttactctttc tcggagtaac tataaccgta atagttatag
    43621 ccgtaactgt aagcggtgct ggcgcgttta atcacaccat tgaggattgc gcctttaata
    43681 ttaacgccag cctgttccag acgctgcatt gacaagctca cctctttggc ggtgttcaag
    43741 ccaaaacgcg caaccagcag gctggtgccg acagagcgtc ccacgaccgc agcatcgctc
    43801 accgccagca tcggcggcgt atcgacaatc accagatcgt aatggtcgtt cgcccattcc
    43861 agtaattgac gcatccgatc gcgcatcagc agttcagacg ggttaggtgg cacctgaccg
    43921 cgagtaatca catcaaagcc tcctttgcca aaatgctgga tcactttgtt gagctcatct
    43981 ttacctgcca gatattccga caagccatgt tcattactca cggtaaacag gttatgcgaa
    44041 taaccacggc gtaagtcggc atcaataaat aacacttttt gatcggactg ggcgatcacc
    44101 gctgccagag ttgaactgac aaacgtttta ccactgtctg gcgtcgcacc ggtgatcatc
    44161 agaatgttat tctccgtctc catcatagcg aagtgcagac tggttcgtag cgcacgtacg
    44221 gcttccacag cagaatccgc cgggttatcc accgccagga aggggatatt tttagtacga
    44281 tggcgctgct gattagaaaa taaatttttc ttacgcagac gggtgcgttt atccagccac
    44341 tcggacattg ggatagtggc ataaacgttg atgccgtgct cttccagttg ttccggtgct
    44401 tctacaccac gacgcaacat cgctcgcgcc agcacggcac ccaccgaaat aaacaggcca
    44461 agaataaaac caagcaccac gttcaacgct tttttcggtt tcactggctg cggctgagtg
    44521 actgccgggt cgataatccg cacgttacca atggcactgg atttcgaaat actcaactcc
    44581 tgctggcggt taagtaattg cagatatacc gcacggcccg cttctacgtc acgacttaaa
    44641 cgcaacactt cctgttgggt ggaaggcata gccgataccc gcttattcag gcgtttgcgt
    44701 tcttgctcca gcgtctggcg tttttccagc agcgcacgat aagttgggtg atctttctta
    44761 tacagctggg agatctctgc ctcgcggaaa gtcagctcat tgagttgatt atcaacgttc
    44821 acaatctgct caagaacggc tttggcttcc aggttaaggt caaccgaatc gcgctgctgg
    44881 cgataaacgt tgagtttttc ttccgcctgg tccagctcgc tgcgcacttc aggcaactgg
    44941 cgctgtaaga attcaaggct ttgtgaatcc tgcgccgcct ggcgagcgat attctgttgc
    45001 aaatagttgt tagcgatgct gttcagaata cgagtaatca actggggatc atcaccggtc
    45061 atggtaagtt ccagcatccc actttcttta ctgcgttcgc taacggtaaa ggtttcctgc
    45121 aacgcgttaa tcgcttccag ttcggtacgc tggctcagga caaactgtgt tcctggtttg
    45181 gccttaatat ccgcgatagt cagcgcaacg ccatcttttt ccagacgctg gccgaccata
    45241 ccattgacgg tgaactcttc accttccagc gtatagtggc cgttttcccc aaccgtgagt
    45301 gtcagttgct gatcctgacc attcagttgt ggaatatgca tccagctgat cgccagctca
    45361 cctggtttct ctttggttaa tctcgcccag ccgcgaccca caatcggaaa atacttctgc
    45421 tcaaccatgt cgcgcagatt cagttcagca atggttttac cgagaatcat gcgcgattgc
    45481 agcagttgga tctccggtgc agactcgggc gatgagttag ggatcatatc gctcaggcca
    45541 ctgagaatgg cgttgccctg tttctgctca acctggacca gagtatctgc ctgataaatt
    45601 ggcgtgctta acagcgagta agcgacagcg atcagcgtga ataacgcggt cacgctgata
    45661 ataaacttac ggtgatccca taactcgccg accagacgaa gcagatcgat ctcattttcc
    45721 tgagtgctgc ctggtggcgt attcatattt ttagttgtca tcgtatttta taaactcaaa
    45781 ttaccggctg aggcgcttcg cccattcctg actggcgcgc tccaacatac cgtagacatg
    45841 ttcaaatgcg tcctgacttt tacgataggg atccgggatc tctttctgtt ccagccattg
    45901 cccaaacagc attgtcttcc cgcggacctc gggagcgatt gcggtaacct gagcaatatg
    45961 ttccgactcc attgccagaa tcagatcgta atttcgtgcc atctcagcag tgagcttacg
    46021 tccggcatgt ccttcaagag atacgccatg attagcggca acatctgccg ctgtcgcatc
    46081 tgcagggtgt tttaccagac catgaacacc tgccgatttg acttttacgc ccggcagacg
    46141 tttacgcagc aagcgctcgc caatgggcga acggcaaatg ttgccggtac aaaccaccag
    46201 gattgagtta aattttagtt gggccatgtt tgaatccacc gtgaagtctc tgtcaggtca
    46261 tgcaccccgg aaatcgtcgg taccagcaga gaaatcacac ggttccaacg agccagcggc
    46321 gcggtagtga cataaacaat gtcgtaaggt tcaagctgga attccgttcc gagtatcatt
    46381 gccgatgcgt ccttcgcatt cagctgataa atattggcga ttttgccatt ctgcttgttt
    46441 tgcgttgcac gaataacaaa aatccccgtc gcatcagcga catcctgatt cataccctca
    46501 gcgttaccca gcgcttccgc cagggtcata ccgctgcggt ccattttcag cgtgctctgt
    46561 ttacccactt cacccatcac gaagactttc agatcatcgt tgcgcggaat aaataaaata
    46621 tcgcccgggt gcagcagttt gttctggcgc aagtcgccac gctgcatcag cgcatagagg
    46681 ttcactttgg tctttacgcc attctgggtg agcaccacgt tgcgccagtc tgcatctgcg
    46741 gtcagaccgc ccgcggcgtt aatcgcatcc atgatggtca gaggaatatt ggtaatcggc
    46801 tgctgacccg atttgctgac ttcgccggtg acgtacgctt tttgcgatcg gaacgccgcg
    46861 acgctgacat cgacttgcgg gctttcaatg actgaatcga gacgcgctgt aatctcgttg
    46921 cgtacctggg taagtgtttt cccggccact ttcaggcgac caatataggg gtagaaaatc
    46981 gcaccatcgg cgttaaccca gttgccggta tcactggcgc tacggtattg ccctgcgggg
    47041 gtggttagtt ccgggtgatc ccagaccgta accatcaaca catcaccaat cccgatacga
    47101 tattcatagt ttgcgatttg ctgctccagc tccgggttag cctgcgacat aatcggtttg
    47161 gcgcgcaact gatcaatcaa tcctggcgtg acgggataga tattgaccat tttgtccagg
    47221 tcatactgat tgtcaggtag ttcaatgaca tccttgttac tggtcgataa attctgacca
    47281 ggaacaaggg tacatgctgt caaagacaga acagccaatg tcagtaccga aaacttaaaa
    47341 atgttctttt tcattggttt atatttatca gtcaatgtaa tgttatgaat tacgtcaggt
    47401 tattgtccaa ccggtacttc tcgctctgcc gtcattggat agagctggta tttgcgtgac
    47461 agcatttgtc caccatcacg cgtcaatggt gtccacgaaa ccaccgcgcg gttgcggtta
    47521 ggtccaatgg tcatcaagtc gaatggaatc gagatataaa aacctttact aaagccgcct
    47581 tcgccgtagt catctttaga tacgttactg attgccgccc atacccctac cgccacgccg
    47641 ctgtcaaagc gtttggcgac gtcgatcgtt gcgcctttat cttttgccag atattgccca
    47701 acgctaagtt tcatcagtac gccattgagc gtcggcgggt tccagtaagc cgtcacgaaa
    47761 cctgttggcg tggaataatc ggtgaaacgc atcatgttgt cccagtcacg ctgcttcacg
    47821 tagttaacgt ccacacccag cgcccagctg gcatctagcg ggcgataaag cagctcggaa
    47881 ccgacaccgg catacatcgt ttccagataa ccgccataca cctggccata gaaaccattg
    47941 cctaagtcag caaaatagtt cgcctgcaag ttgttgagat aaacgtcatt gcgaacgtag
    48001 tcacggatat gcgtgcgcac gcggggcagg gtagagtccg cgggcaacag cgaagactta
    48061 aacttgtcgt agttgttgta aatattggtg aaaataccgc catcaagcag caggtggtcg
    48121 gtaaaccagt agcgggcact ggacatcagc cccagctgga acatatagaa atcttccggg
    48181 ccgcccagcg actgtgaaag tgttgggttg aaagagtagc taaagcgatc ttcacgaata
    48241 cggtagcccc gaccaaaggc agaaagatct tctgcttcaa cacgttgttg ttgcagttgc
    48301 tctgattgac caggcgctgt accagccagc tgtttgcgca ggctggctac gtcggtttcg
    48361 gtagtcacca tcgccatatg ctcgcgcttt tgcgtcacgc taatcttctc aacgccttgc
    48421 ggcaggttat tcaccagaat ccggttggca cgatcgaccg cttcacgaga gtcacggtat
    48481 ttgtattgct gaccagacat atacagcgtc ttatcgcgta gctgaatttc tggcgcgtca
    48541 aagcccgcgt tatacttcag ggcggtaagt tggtttgcta ccgtggtgta ctgcaatcct
    48601 tcagattcag gcgcaggttg atatgccggt tttggcgtat cgcgcagggc agggcgcaga
    48661 tcgttgaaat tggtccgtaa agtgaaacca aacatcaacg tgttaccgcg ttcataactc
    48721 aggttgagat ctgcccagct ggcagcgcga taaactgcac cgacgttgaa atggcttgcc
    48781 tgaggcagtt tgccagcgaa atcattctgg taattgttgc cgtcgtattc gagtttcaga
    48841 cgcaggggat tccacggcgt ttgatactca atgccgccaa agatggaagc cgggccacga
    48901 aagatatcgc taaagctgat atcgcccgca tcgtgagact ctgcgcgatg acaatattta
    48961 tcgctcaccc ggcaaaacgg gttggtaata ttgcccgcat taccggcgta gccccatgcc
    49021 atcccgaggg tgaaatcaaa tggccccgct tgcttgctgg ccaccagata ctcaccgtca
    49081 aacagacccg taccagcaat atcacgttta ccaaacgcca cttgcggtag ccagtgacct
    49141 tcttcccaca ggcgaagttt aaaatcgaat gatttgtctt tgtatgactg atcgccgctg
    49201 aaatcttccc actggctata tttgcgtgtg cgcacatccg tataacgaat ggtgccttcc
    49261 agccatggga acagcgccac ggaggtggag tagaaccggt attgatcgtt atcccggtag
    49321 ttgacgctga attcaccttc cggtgcgatg cgcgcatttg gcatctgcaa caatcctgtg
    49381 ccgccaaagt ctgattgcga cgaacccagc ggatccgggt aggttaatac ttcagcatga
    49441 caggcactgg agacggcaat ggccaggcag cttaaaagat aagaattctt cttcatcagt
    49501 caggaacacg ttgcgttaaa acagacacaa tctggtcatt taaatcagca tatttttcgg
    49561 gcagtacatg cgcagaaaag cccaaccaaa gctggctgcc aggcggtggt tccacatgtc
    49621 ttttattcca cagggcaaca ggggcgacga cagtctcacc ctcaggcgta atcaccataa
    49681 cgttattctt gtccgcgcct gcaaggcggg ggtgatcttg cagataatcg gtaacgctac
    49741 ggcctgcctg ccagggcaat tgcccggccc cagaaaccgc acccagcaga gtaatagtca
    49801 ccggacgctg tacggtatag agcgtatagt ccccgaccaa cgggggattg ctgttctcat
    49861 ccactcgtac aaaatcaggg tccagtttga ccggtaagcg accagtgata ttgagattga
    49921 gtaactgctg gcgcacggat ttaatcgttg ccgccacatc gtcgtcagct tctgcttccc
    49981 aggaagcaag ctgtgccatg acatgttggt agtctttaag cgctttagct ttcgccgcag
    50041 agtcggtcag caaggcacca ggccaccaga ggcgatcgcg cagttgcggt tgtgtcacca
    50101 actggacaac attttccacg gggccaacgg aaagtgtctg ttgttcgcca ggcagataaa
    50161 tagtcaccgt tccttgcgca aaggcatggg gtgtcattac gtaaagtacg ctggcaatga
    50221 aatacgactg taatttattc atggtgcggg tttaagaaac gtcatttcta cgggaataac
    50281 ccctgcgcct aacatttgcc gactttgacg aacctggcca gtagccgaat cgacccagaa
    50341 tgtgttttgc cactgttttt ctggccgcgt ggaagtcacc tcttcctgcc agacattaca
    50401 cagcacattg cgacccgcaa tattcagcgt ctctaatcca gcaaaggaaa aagtagaact
    50461 cagggtcgca gagcgaaaat ggttgtcttc actccagaga atatcgcgcg tccagcggct
    50521 gccatctttt attgccagcg cgttacgcag agggtcctgc ccggaatttg tcacttccag
    50581 cagattatta ttaagtttga cggtttttaa aagacgtcca ttgtgggtaa ccagcatggc
    50641 gttatcctgg gataaccatt tgctttgttc ttgttctata tatcccagta ccacaaagat
    50701 tcgttgccct tcattaaggc gtaaatacat cgtggaataa ggcaacgcct ggatctgctg
    50761 atccgctacg gtgatatcct gattatcgaa aaggctggcg cgaaatgtat cgaccatact
    50821 ttgctggctg tgcgtacatc ccgcaagaaa tagtgcgaaa atcgatagaa taagagggcg
    50881 cacctttaat ccttgaaagg ttaattaaaa aaatgcaaat ctctaaagcc cggtacgaat
    50941 atcgcaaccg ggtatcattt aactttaatt tcgacgcgtt aaagcagtat tagcgtgtag
    51001 tggacgtggt ggttgaggtg ttatgagaac cgccatcgcc actgtcattt gctgcaacaa
    51061 ctgccaacgt cgccacacca acagccgttg ccacaccgac aaccgtcgcc gtgccgacag
    51121 cgccagcagc cacgccacca gtggtagccg cagtcgtggt tgcaccggta gtggtcgttg
    51181 tggtgccagt agtacccgtc gttgcctcgg tcccgtttgc tgcttcaggg gcagcaaatg
    51241 cagctggcgt cgtcaacatg aaggccataa ggattgcaga aagcttgtgt ttcataactt
    51301 ttcctttatt catcgcatgg acaatacgta cgcttgagat caggattgtc tactttgagt
    51361 ataacatcct tgtgaaatct ccctttcctc ataccgcttg cactatatac tgcgggtagg
    51421 aaaggcgcgc agagggaaat ataagattgt taactatccc cctctggaaa tacga
//
LOCUS       DBANUU010000014        50430 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000014 DBANUU010000000
VERSION     DBANUU010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.014"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            491..2059
                     /gene="cydA"
                     /locus_tag="ACIE68_000791"
     CDS             491..2059
                     /gene="cydA"
                     /locus_tag="ACIE68_000791"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFX5907155.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGVSAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            2075..3214
                     /gene="cydB"
                     /locus_tag="ACIE68_000792"
     CDS             2075..3214
                     /gene="cydB"
                     /locus_tag="ACIE68_000792"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFX5907156.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3229..3342
                     /gene="cydX"
                     /locus_tag="ACIE68_000793"
     CDS             3229..3342
                     /gene="cydX"
                     /locus_tag="ACIE68_000793"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFX5907157.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3342..3635
                     /gene="ybgE"
                     /locus_tag="ACIE68_000794"
     CDS             3342..3635
                     /gene="ybgE"
                     /locus_tag="ACIE68_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFX5907158.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3785..4189
                     /gene="ybgC"
                     /locus_tag="ACIE68_000795"
     CDS             3785..4189
                     /gene="ybgC"
                     /locus_tag="ACIE68_000795"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFX5907159.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            4186..4878
                     /gene="tolQ"
                     /locus_tag="ACIE68_000796"
     CDS             4186..4878
                     /gene="tolQ"
                     /locus_tag="ACIE68_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFX5907160.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4882..5310
                     /gene="tolR"
                     /locus_tag="ACIE68_000797"
     CDS             4882..5310
                     /gene="tolR"
                     /locus_tag="ACIE68_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFX5907161.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5375..6640
                     /gene="tolA"
                     /locus_tag="ACIE68_000798"
     CDS             5375..6640
                     /gene="tolA"
                     /locus_tag="ACIE68_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFX5907162.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6773..8065
                     /gene="tolB"
                     /locus_tag="ACIE68_000799"
     CDS             6773..8065
                     /gene="tolB"
                     /locus_tag="ACIE68_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFX5907163.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            8100..8621
                     /gene="pal"
                     /locus_tag="ACIE68_000800"
     CDS             8100..8621
                     /gene="pal"
                     /locus_tag="ACIE68_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFX5907164.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8631..9422
                     /gene="cpoB"
                     /locus_tag="ACIE68_000801"
     CDS             8631..9422
                     /gene="cpoB"
                     /locus_tag="ACIE68_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFX5907165.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9587..9662
                     /locus_tag="ACIE68_000802"
     tRNA            9587..9662
                     /locus_tag="ACIE68_000802"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9620..9622,aa:Lys,seq:ttt)
     gene            9798..9873
                     /locus_tag="ACIE68_000803"
     tRNA            9798..9873
                     /locus_tag="ACIE68_000803"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9831..9833,aa:Val,seq:tac)
     gene            9876..9951
                     /locus_tag="ACIE68_000804"
     tRNA            9876..9951
                     /locus_tag="ACIE68_000804"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9909..9911,aa:Lys,seq:ttt)
     gene            10101..10176
                     /locus_tag="ACIE68_000805"
     tRNA            10101..10176
                     /locus_tag="ACIE68_000805"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10134..10136,aa:Val,seq:tac)
     gene            10180..10255
                     /locus_tag="ACIE68_000806"
     tRNA            10180..10255
                     /locus_tag="ACIE68_000806"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10213..10215,aa:Lys,seq:ttt)
     gene            10402..10477
                     /locus_tag="ACIE68_000807"
     tRNA            10402..10477
                     /locus_tag="ACIE68_000807"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10435..10437,aa:Lys,seq:ttt)
     gene            10511..10586
                     /locus_tag="ACIE68_000808"
     tRNA            10511..10586
                     /locus_tag="ACIE68_000808"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10544..10546,aa:Lys,seq:ttt)
     gene            10617..10742
                     /locus_tag="ACIE68_000809"
     ncRNA           10617..10742
                     /ncRNA_class="other"
                     /locus_tag="ACIE68_000809"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            10991..12034
                     /gene="nadA"
                     /locus_tag="ACIE68_000810"
     CDS             10991..12034
                     /gene="nadA"
                     /locus_tag="ACIE68_000810"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFX5907166.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSISV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            12072..12791
                     /gene="pnuC"
                     /locus_tag="ACIE68_000811"
     CDS             12072..12791
                     /gene="pnuC"
                     /locus_tag="ACIE68_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFX5907167.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(12788..13729)
                     /gene="zitB"
                     /locus_tag="ACIE68_000812"
     CDS             complement(12788..13729)
                     /gene="zitB"
                     /locus_tag="ACIE68_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFX5907168.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVFGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13843..14223)
                     /gene="ybgS"
                     /locus_tag="ACIE68_000813"
     CDS             complement(13843..14223)
                     /gene="ybgS"
                     /locus_tag="ACIE68_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFX5907169.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14539..15591
                     /gene="aroG"
                     /locus_tag="ACIE68_000814"
     CDS             14539..15591
                     /gene="aroG"
                     /locus_tag="ACIE68_000814"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFX5907170.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(15749..16501)
                     /gene="gpmA"
                     /locus_tag="ACIE68_000815"
     CDS             complement(15749..16501)
                     /gene="gpmA"
                     /locus_tag="ACIE68_000815"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFX5907171.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(16706..17746)
                     /gene="galM"
                     /locus_tag="ACIE68_000816"
     CDS             complement(16706..17746)
                     /gene="galM"
                     /locus_tag="ACIE68_000816"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFX5907172.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(17740..18888)
                     /gene="galK"
                     /locus_tag="ACIE68_000817"
     CDS             complement(17740..18888)
                     /gene="galK"
                     /locus_tag="ACIE68_000817"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFX5907173.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18892..19938)
                     /gene="galT"
                     /locus_tag="ACIE68_000818"
     CDS             complement(18892..19938)
                     /gene="galT"
                     /locus_tag="ACIE68_000818"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFX5907174.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19948..20964)
                     /gene="galE"
                     /locus_tag="ACIE68_000819"
     CDS             complement(19948..20964)
                     /gene="galE"
                     /locus_tag="ACIE68_000819"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFX5907175.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(21225..22697)
                     /gene="modF"
                     /locus_tag="ACIE68_000820"
     CDS             complement(21225..22697)
                     /gene="modF"
                     /locus_tag="ACIE68_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFX5907176.1"
                     /translation="MSSLQILQGAFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            complement(22765..23553)
                     /gene="modE"
                     /locus_tag="ACIE68_000821"
     CDS             complement(22765..23553)
                     /gene="modE"
                     /locus_tag="ACIE68_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFX5907177.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VIMSLPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            23682..23831
                     /gene="acrZ"
                     /locus_tag="ACIE68_000822"
     CDS             23682..23831
                     /gene="acrZ"
                     /locus_tag="ACIE68_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFX5907178.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVVKKDQ
                     PGQNH"
     gene            23998..24771
                     /gene="modA"
                     /locus_tag="ACIE68_000823"
     CDS             23998..24771
                     /gene="modA"
                     /locus_tag="ACIE68_000823"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFX5907179.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVAIFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            24771..25460
                     /gene="modB"
                     /locus_tag="ACIE68_000824"
     CDS             24771..25460
                     /gene="modB"
                     /locus_tag="ACIE68_000824"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFX5907180.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            25463..26521
                     /gene="modC"
                     /locus_tag="ACIE68_000825"
     CDS             25463..26521
                     /gene="modC"
                     /locus_tag="ACIE68_000825"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFX5907181.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(26522..27340)
                     /gene="ybhA"
                     /locus_tag="ACIE68_000826"
     CDS             complement(26522..27340)
                     /gene="ybhA"
                     /locus_tag="ACIE68_000826"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFX5907182.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            27495..28490
                     /gene="pgl"
                     /locus_tag="ACIE68_000827"
     CDS             27495..28490
                     /gene="pgl"
                     /locus_tag="ACIE68_000827"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFX5907183.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYSIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(28531..29484)
                     /gene="ybhD"
                     /locus_tag="ACIE68_000828"
     CDS             complement(28531..29484)
                     /gene="ybhD"
                     /locus_tag="ACIE68_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFX5907184.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            29668..30720
                     /gene="ybhH"
                     /locus_tag="ACIE68_000829"
     CDS             29668..30720
                     /gene="ybhH"
                     /locus_tag="ACIE68_000829"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFX5907185.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            30796..32229
                     /gene="ybhI"
                     /locus_tag="ACIE68_000830"
     CDS             30796..32229
                     /gene="ybhI"
                     /locus_tag="ACIE68_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFX5907186.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            32412..34673
                     /gene="ybhJ"
                     /locus_tag="ACIE68_000831"
     CDS             32412..34673
                     /gene="ybhJ"
                     /locus_tag="ACIE68_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFX5907187.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYKIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(34907..36190)
                     /gene="ybhC"
                     /locus_tag="ACIE68_000832"
     CDS             complement(34907..36190)
                     /gene="ybhC"
                     /locus_tag="ACIE68_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFX5907188.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(36325..37395)
                     /locus_tag="ACIE68_000833"
     CDS             complement(36325..37395)
                     /locus_tag="ACIE68_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFX5907189.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTALHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(37373..37591)
                     /locus_tag="ACIE68_000834"
     CDS             complement(37373..37591)
                     /locus_tag="ACIE68_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFX5907190.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(37631..37798)
                     /locus_tag="ACIE68_000835"
     CDS             complement(37631..37798)
                     /locus_tag="ACIE68_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5907191.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMLWAQIAHADITNI
                     RIEELKEHQAA"
     gene            complement(37887..38168)
                     /locus_tag="ACIE68_000836"
     CDS             complement(37887..38168)
                     /locus_tag="ACIE68_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5907192.1"
                     /translation="MSIHDLCEDQEQWAMQTLMGSGVLARCRIHNDVILDSGNDASSA
                     YKLGTYLYQKDNSCNLFNTLTEARDAIKDAYESYCGIDDCPQCSKYIDD"
     gene            complement(38283..38852)
                     /locus_tag="ACIE68_000837"
     CDS             complement(38283..38852)
                     /locus_tag="ACIE68_000837"
                     /inference="COORDINATES: protein motif:HMM:NF016342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dATP/dGTP pyrophosphohydrolase domain-containing
                     protein"
                     /protein_id="HFX5907193.1"
                     /translation="MASLEREQIRHEHAKWSDSTFGCVGPIGPLKHLSKEALEAAAEP
                     DDLSEWADMQFLLWDAQRRAGISDAEITAAMEDKLKINMERQWPEPKDGEPRLHIKEP
                     GNYPVTPDGWISCSERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGDWFNDGNPT
                     WDDGDGEDLYLKEVTHWMPLPEPPRLKEL"
     gene            complement(38826..38951)
                     /locus_tag="ACIE68_000838"
                     /pseudo
     CDS             complement(38826..38951)
                     /locus_tag="ACIE68_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF550 domain-containing protein"
     gene            complement(38953..39324)
                     /locus_tag="ACIE68_000839"
     CDS             complement(38953..39324)
                     /locus_tag="ACIE68_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFX5907194.1"
                     /translation="METVATPQKLLAFRMKVTPQVVLALLDERERNQQYIKRRDQENE
                     DIALTVGKLRVELEAEKQRAKDLFMENARLKSGIAGLIHLGIRYADVEVMRIAGDAQL
                     STPCTDSIINSIATGIRIKGE"
     gene            complement(39366..39587)
                     /locus_tag="ACIE68_000840"
     CDS             complement(39366..39587)
                     /locus_tag="ACIE68_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFX5907195.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLVVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(39686..39901)
                     /locus_tag="ACIE68_000841"
     CDS             complement(39686..39901)
                     /locus_tag="ACIE68_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFX5907196.1"
                     /translation="MTPQQENALRSIARLANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(39978..40169)
                     /locus_tag="ACIE68_000842"
     CDS             complement(39978..40169)
                     /locus_tag="ACIE68_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFX5907197.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMVAKAEADERDQV"
     gene            complement(40142..40324)
                     /locus_tag="ACIE68_000843"
     CDS             complement(40142..40324)
                     /locus_tag="ACIE68_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFX5907198.1"
                     /translation="MKHPHDNIRVGAITFVYSVTKRGWVFPGLPVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLS"
     gene            complement(40321..41001)
                     /locus_tag="ACIE68_000844"
     CDS             complement(40321..41001)
                     /locus_tag="ACIE68_000844"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFX5907199.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARALFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMANFDEMVPEFIEKMDVALAEIG
                     FVFGEQWR"
     gene            complement(40998..41783)
                     /gene="bet"
                     /locus_tag="ACIE68_000845"
     CDS             complement(40998..41783)
                     /gene="bet"
                     /locus_tag="ACIE68_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFX5907200.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            complement(41789..42085)
                     /locus_tag="ACIE68_000846"
     CDS             complement(41789..42085)
                     /locus_tag="ACIE68_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFX5907201.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRCGASKKAITRAFDDDVEFQERMAEHIRFMVETIAHHQVDIDSEV"
     gene            complement(42160..42303)
                     /gene="kil"
                     /locus_tag="ACIE68_000847"
     CDS             complement(42160..42303)
                     /gene="kil"
                     /locus_tag="ACIE68_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFX5907202.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
     gene            complement(42272..42436)
                     /locus_tag="ACIE68_000848"
     CDS             complement(42272..42436)
                     /locus_tag="ACIE68_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFX5907203.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNTYGYPD"
     gene            complement(42509..42877)
                     /locus_tag="ACIE68_000849"
     CDS             complement(42509..42877)
                     /locus_tag="ACIE68_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFX5907204.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSLLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDSDDMTIKAA"
     gene            complement(43073..43522)
                     /locus_tag="ACIE68_000850"
     CDS             complement(43073..43522)
                     /locus_tag="ACIE68_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5907205.1"
                     /translation="MEEQANKILVELLQKASNGIDAAVSFSQAQIPDVVHQLLLWNMV
                     DSLIKTLIAILTIPLVFWFMKKQCQRVETGKIGDEGYSWEKGNPKYRPTMVWDSKGDI
                     NLLIMPLVGVLTLWGIFIIGVVTNMTWLKIWLAPKLYLIEYAASLVK"
     gene            complement(43581..43678)
                     /locus_tag="ACIE68_000851"
                     /pseudo
     CDS             complement(43581..43678)
                     /locus_tag="ACIE68_000851"
                     /inference="COORDINATES: protein motif:HMM:NF022510.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction Ral family protein"
     gene            43945..44427
                     /locus_tag="ACIE68_000852"
     CDS             43945..44427
                     /locus_tag="ACIE68_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006641566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion B family protein"
                     /protein_id="HFX5907206.1"
                     /translation="MYMIVIWVGLLLLSPDNWPEYVNERIGIPHVWHVFVFALAFSLA
                     INVHRLSAIASARYKRFKLRKRIKMQNDKVRSVIQNLTEEQSMVLCAALNEGRKYVVT
                     SKQFPYISELIELGVLNKTFSRWNGKHILFPIEDIYWTELVASYDPYNIEIKPRPISK
                     "
     gene            complement(44428..44775)
                     /locus_tag="ACIE68_000853"
                     /pseudo
     CDS             complement(44428..44775)
                     /locus_tag="ACIE68_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
     gene            complement(45184..45936)
                     /locus_tag="ACIE68_000854"
     CDS             complement(45184..45936)
                     /locus_tag="ACIE68_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
                     /protein_id="HFX5907207.1"
                     /translation="MMCSTVISQLGFECHPIGKTLRIISPFTYCDDGEHVGAFIREVN
                     GRYLVSDRCDALMNMEARGISLTKKRLDEIRQLLLKEGAELNARGEIIAWATEKDVGA
                     ITSNIIRAGILASTLSLDWYQPVQAEKFESMVIDYLYHTELREALSLRENVYGLSGHQ
                     ITVPVTIKTDIPKYVFTSSVKHGGSWNSAYSLLGKLIDLKASSEEYNNRFVVIDSEAI
                     GDQMQQLSLLFHESSQVLPFSKRETWIKRLAA"
     gene            complement(45933..46490)
                     /locus_tag="ACIE68_000855"
     CDS             complement(45933..46490)
                     /locus_tag="ACIE68_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5907208.1"
                     /translation="MAKIDDYQPSQVEVDKVLYCKKIVNFSGVKWKQKPSRSDMWLQA
                     HIIPLDEDCIPIQGLKFELKWKPDQDSEPDDPISYPKINIIAFYHNKRVFAVDTYHFD
                     KHTNSYKVDHPKYQDIIYGAHYHVYYEEAGYYSDRIAFPIEDDINPDDLVGYWNYFCK
                     HLNITYSGRIPLPLEDESGQMGFGI"
     gene            complement(46530..47243)
                     /locus_tag="ACIE68_000856"
     CDS             complement(46530..47243)
                     /locus_tag="ACIE68_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFX5907209.1"
                     /translation="MSAKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG
                     QSGVGALFNGINALNAYNAALLAKILNVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY
                     EYPVFSHVQAGMFSPELRTFTKSDAEKWVSTTKKASGSAFWLEVEGNSMTAPTGYKPS
                     FPDGMLILVDPEQTVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE
                     SCSVVGKVIASQWPEETFG"
     gene            47344..47544
                     /locus_tag="ACIE68_000857"
     CDS             47344..47544
                     /locus_tag="ACIE68_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFX5907210.1"
                     /translation="MEQRITLKDYAMRFGQTKTAKDLGVYQSAINKAIHAGRKIFLTI
                     NADGSVYAEEVKPFPSNKKTTA"
     gene            47663..47956
                     /locus_tag="ACIE68_000858"
     CDS             47663..47956
                     /locus_tag="ACIE68_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFX5907211.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVAAILTNKKRPAATERSEQIQMEF"
     gene            47989..48888
                     /locus_tag="ACIE68_000859"
     CDS             47989..48888
                     /locus_tag="ACIE68_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFX5907212.1"
                     /translation="MTNTAKILNFGRGNFAGQERNVADLDDGYARLSNMLLEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ
                     GGMFGPNKNISEWCIPQNEGKSPKTRDKTSLKLGDCYPSKQGDTKDTITKEKRKDYSS
                     ENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFA
                     GWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQ
                     AVVTASKPKLDLTNTDWIYGVDL"
     gene            48885..49586
                     /locus_tag="ACIE68_000860"
     CDS             48885..49586
                     /locus_tag="ACIE68_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFX5907213.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNEVNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAELKAKFGLKGASV"
     gene            49583..49873
                     /locus_tag="ACIE68_000861"
     CDS             49583..49873
                     /locus_tag="ACIE68_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFX5907214.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATKEEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            49947..50387
                     /locus_tag="ACIE68_000862"
     CDS             49947..50387
                     /locus_tag="ACIE68_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFX5907215.1"
                     /translation="MKKLTFEIRSPAHQQNAIQAIQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVSEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
BASE COUNT        12382 a        12176 c        12727 g        13145 t
ORIGIN      
        1 gtcaaccgtg ctgtttttgc ttcgtctctt tttatcttta attgccaacc gaaactaatt
       61 tcagccttat aactcacaca ttttaaacat aaatgtcact aaagttacct tattgaaaca
      121 tgattaacat aatttgtagg aattgatatt tatcaatgta taagtcttgg aaatgggcat
      181 caaaaagaga taaattgttc tcgatcaaat tggctgaaag gcggtaattt agctataaat
      241 tgatcaccgt cgaaaaatgc aaatttgctt caacaaaaac ctgtttattg taaggatttt
      301 gcggcgtaat atatacgtgg gatcaatttg agtttttatt aacatgtttg caacctttct
      361 ttacgccgtt tttgtgtgca ttcacatggt atgatgaaag tgttcaaaca aatttctatt
      421 ggggcatgcg tgtgaccctt tctaacgggg ttcactctcg gagtcttcat gcgatgagca
      481 aggagtcatg atgttagata tagtcgaact gtcgcgctta cagtttgcct tgaccgcgat
      541 gtaccacttc ctttttgtgc cactgacgct cggtatggcg ttcctgctgg ccattatgga
      601 aacggtctac gtcctctccg gcaaacagat ttataaagat atgaccaagt tctggggcaa
      661 gttgtttggt atcaacttcg ctctgggggt ggctaccggt ctgaccatgg agttccagtt
      721 cgggactaac tggtcttact attcccacta tgtaggggat atcttcggtg cgccgctggc
      781 aatcgaaggt ctgatggcct tcttcctcga atccaccttt gtaggtctgt tcttcttcgg
      841 ttgggatcgt ctgggtaaag ttcagcatat gtgtgtcacc tggctggtgg cgctcggttc
      901 aaacctgtcc gcactgtgga ttctggttgc gaacggctgg atgcaaaacc caatcgcgtc
      961 cgatttcaac tttgaaacta tgcgtatgga gatggtgagc ttctccgagc tggtgcttaa
     1021 cccggttgct caggtgaaat tcgttcacac tgtagcgtct ggttatgtta ctggcgcgat
     1081 gttcatcctc ggtgtcagcg catggtatat gctgaaaggt cgtgacttcg ccttcgctaa
     1141 acgctccttt gctatcgctg ccagcttcgg tatggctgct gttctgtctg ttattgtttt
     1201 gggtgatgaa tccggctacg aaatgggcga cgtgcagaaa accaaactgg ctgctattga
     1261 agccgagtgg gaaacgcaac ctgcgcctgc tgcctttact ctgttcggca ttcctgatca
     1321 ggaagaggag acgaacaaat ttgcgattca gatcccttac gcactgggca tcattgcaac
     1381 gcgttccgtg gataccccgg ttatcggcct gaaagagctg atggtgcagc atgaagaacg
     1441 cattcgtaac gggatgaagg cgtactctct gctcgaacaa ctgcgttctg gttctaccga
     1501 ccaggcggtt cgtgaccagt tcaatagcat gaagaaagac ctcggttacg gtctgctgct
     1561 gaaacgctat acgccaaacg tggctgatgc gactgaagcg cagattcaac aggcaaccaa
     1621 agactccatc ccgcgtgtag cgccgctgta ctttgcgttc cgtatcatgg tggcgtgtgg
     1681 cttcctgctt ctggcaatca tcgcgctctc tttctggagt gtcatccgca accgcattgg
     1741 cgagaaaaaa tggcttctgc gcgccgcgct gtacggtatt ccgctgccgt ggattgctgt
     1801 agaagcgggc tggttcgtgg ctgaatatgg ccgccaaccg tgggctatcg gtgaagtgct
     1861 gccgacagct gtggcgaact cgtcactgac cgcaggcgat ctcatcttct caatggtgct
     1921 gatttgcggc ctgtataccc tgttcctggt ggcagaattg ttcttaatgt tcaaatttgc
     1981 acgcctcggc ccaagcagcc tgaaaaccgg tcgctatcac tttgagcagt cttccacgac
     2041 tactcagccg gcacgctaag acaggagtcg tcaaatgatc gattatgaag tattgcgttt
     2101 tatctggtgg ctgctggttg gcgttctgct gattggtttt gcagtcactg acggtttcga
     2161 catgggggtg ggcatgctca cccgtttcct cggtcgtaac gacaccgagc gtcgaattat
     2221 gattaactcc attgcaccac actgggacgg taaccaggtt tggctgatca ccgcgggcgg
     2281 cgcactcttt gctgcctggc cgatggtcta tgccgctgcg ttctccggct tctatgtggc
     2341 gatgatcctc gtgctggcgt ctttgttctt ccgtccggtc ggttttgact accgctccaa
     2401 gattgaagaa acccgctggc gtaacatgtg ggactggggc atcttcattg gtagcttcgt
     2461 tccgccgctg gtaattggtg tagcgttcgg taacctgttg cagggcgtac cgttcaacgt
     2521 tgatgaatat ctgcgtctgt actacaccgg taacttcttc cagttgctta acccgttcgg
     2581 cctgctggca ggcgtggtga gcgtagggat gatcattact cagggcgcaa cctatctgca
     2641 aatgcgtacc gtgggcgaac tgcacctgcg tacccgtgca acggctcagg tggctgcgct
     2701 ggtgacactg gtctgtttcg cactggctgg cgtatgggtg atgtacggta tcgatggtta
     2761 tgtcgtgaaa tcgacaatgg accattacgc agcctctaac ccactgaata aagaagtggt
     2821 tcgtgaagct ggcgcatggc tggttaactt caacaacacg ccaattctgt gggctattcc
     2881 ggcactgggt gtggttctgc cgctgctgac catcctgact gcacgtatgg ataaagccgc
     2941 gtgggcgttt gtgttctcct ccctgacgct ggcctgcatc atcctgacag ccggtatcgc
     3001 aatgttcccg tttgtgatgc cgtccagcac catgatgaac gcaagtctga caatgtggga
     3061 tgcaacttcc agccagctga cgcttaacgt catgacctgg gttgcggtgg ttctggtacc
     3121 gatcattctg ctctacaccg cctggtgtta ctggaaaatg ttcggtcgta tcaccaaaga
     3181 agatattgaa cgtaacaccc actctctgta ctaagtaagg agctaaaaat gtggtatttc
     3241 gcatggattc tgggaacgct tcttgcctgt tcgtttgggg taatcaccgc gctggcgctt
     3301 gaacacgtcg aatcaggcaa agccggtcaa gaagacatct gatgagtaag attatcgcga
     3361 ctttgtatgc ggtaatggac aagcgccccc tgcgggcgct ttccttcgtg atggcgcttc
     3421 tgttagcagg atgtatgttt tgggacccat ctcgttttgc cgcgaagacc agtgaactgg
     3481 aaatctggca tggtttattg ctgatgtggg ccgtctgtgc tggtgtgatt cacggcgtgg
     3541 gctttcgtcc gcagaaggtt ctttggcaag ggattttttg cccattgctt gccgatattg
     3601 ttctcattgt cgggctgatt ttcttcttct tttaaatcag aattctcttt aaaaaattat
     3661 gggccgctcc aggcccataa atttttacgc tcccttaact tgccctcatt cccaaacctc
     3721 aatcgcgcgc gtatagtagc agcgtttaaa agctctaact tttgttgcat taccgggatg
     3781 taaagtgaat acaacgctgt ttcgatggcc ggttcgcgtc tactatgaag ataccgatgc
     3841 cggtggtgtg gtgtaccacg ccagttacgt cgctttttat gaaagagcac gcacagagat
     3901 gctgcgtcat catcacttca gtcagcaggc gctgatggct gaacgcgttg cctttgtggt
     3961 acgtaaaatg acggtggaat attacgcacc tgcgcggctc gacgatatgc tcgaaataca
     4021 gactgaaata acatcaatgc gtggcacctc tttggttttc acgcaacgta ttgtcaacgc
     4081 cgagaatact ttgctgaatg aagcagaggt tctggttgtt tgcgttgacc cactcaaaat
     4141 gaagcctcgt gcgcttccca agtctattgt cgcggagttt aagcagtgac tgacatgaat
     4201 atccttgatt tgttcctgaa ggctagcctt ctggttaaac ttatcatgtt gattttgatt
     4261 ggtttttcaa tcgcatcttg ggccattatt atccagcgga cccgtattct taacgcagcg
     4321 gcgcgcgaag ccgaagcgtt tgaagataaa ttctggtctg gaatcgaact ctctcgcctc
     4381 tatcaagaga gccaggggaa acgggataat ctgactggtt cggaacaaat cttttacagc
     4441 gggttcaaag agtttgtgcg cctgcatcgt gccaatagcc atgcgccgga agccgtagtg
     4501 gaaggggcgt cgcgtgctat gcgtatctcc atgaaccgtg aacttgaaaa tctggaaacg
     4561 cacattccgt tcctcggtac ggttggctcc atcagcccgt atattggtct gtttggtacg
     4621 gtctggggga tcatgcacgc ctttatcgcc ctcggggcgg taaaacaagc aacactgcaa
     4681 atggttgcgc ccggtatcgc agaagcgttg attgcgactg caattggtct gttcgccgca
     4741 attccggcgg taatggctta taaccgcctc aaccagcgcg taaacaaact ggaactgaat
     4801 tacgacaact ttatggaaga gtttaccgcg attctgcacc gccaggcgtt taccgttagc
     4861 gagagcaaca aggggtaagc catggccaga gcgcgtggac gaggtcgtcg cgatctcaag
     4921 tccgaaatca acattgtacc gttgctggac gtactgctgg tgctgttgct gatctttatg
     4981 gcgacagcgc ccatcatcac ccagagcgtg gaggtcgatc tgccagacgc tactgaatca
     5041 caggcggtga gcagtaacga taatccgcca gtgattgttg aagtgtctgg tattggtcag
     5101 tacaccgtgg tggttgagaa agatcgcctg gagcgtttac caccagagca ggtggtggcg
     5161 gaagtgtcca gccgtttcaa ggccaacccg aaaacggtct ttctgatcgg tggcgcaaaa
     5221 gatgtgcctt acgatgaaat aattaaagca ctgaacttgt tacatagtgc gggtgtgaaa
     5281 tcggttggtt taatgacgca gcctatctaa acatctgcgt ttcccttgct tgaaagagag
     5341 cgggtaacag gcgaacagtt tttggaaacc gagagtgtca aaggcaaccg aacaaaacga
     5401 caagctcaag cgggcgataa ttatttcagc agtgctgcat gtcatcttat ttgcggcgct
     5461 gatctggagt tcgttcgatg agaatataga agcttcagcc ggaggcggcg gtggttcgtc
     5521 tatcgacgct gtcatggttg attcaggtgc ggtagttgag cagtacaaac gcatgcaaag
     5581 ccaggaatca agcgcgaagc gttctgatga acagcgcaag atgaaggaac agcaggctgc
     5641 tgaagaactg cgtgagaaac aagcggctga acaggaacgc ctgaagcaac ttgagaaaga
     5701 gcggttagcg gctcaggagc agaaaaagca ggctgaagaa gccgcaaaac aggccgagtt
     5761 aaagcagaag caagctgaag aggcggcagc gaaagcggcg gcagatgcta aagcgaaggc
     5821 cgaagcagat gctaaagctg cggaagaagc agcgaagaaa gcggctgcag acgcaaagaa
     5881 aaaagcagaa gcagaagccg ccaaagccgc agccgaagcg cagaaaaaag ccgaggcagc
     5941 cgctgcggca ctgaagaaga aagcggaagc ggcagaagca gctgcagctg aagcaagaaa
     6001 gaaagcggca actgaagctg ctgaaaaagc caaagcagaa gctgagaaga aagcggctgc
     6061 tgaaaaggct gcagctgata agaaagcggc agcagagaaa gctgcagccg acaaaaaagc
     6121 agcagaaaaa gcggctgctg aaaaggcagc agctgataag aaagcagcgg cagaaaaagc
     6181 cgccgcagac aaaaaagcgg cagcggcaaa agctgcagct gaaaaagccg ctgcagcaaa
     6241 agcggccgca gaggcagatg atattttcgg tgagctaagc tctggtaaga atgcaccgaa
     6301 aacgggggga ggggcgaaag ggaacaatgc ttcgcctgcc gggagtggta atactaaaaa
     6361 caatggcgca tcaggggccg atatcaataa ctatgccggg cagattaaat ctgctatcga
     6421 aagtaagttc tatgacgcat cgtcctatgc aggcaaaacc tgtacgctgc gcataaaact
     6481 ggcacccgat ggtatgttac tggatatcaa acctgaaggt ggcgatcccg cactttgtca
     6541 ggctgcgttg gcagcagcta aacttgcgaa gatcccgaaa ccaccaagcc aggcagtata
     6601 tgaagtgttc aaaaacgcgc cattggactt caaaccgtaa tcgcgatgtt gactgttcgg
     6661 acggtcaaca tcaggcaccg gttgccacgg ggttctggta gttttgtgta ttttagtttg
     6721 ttaacattct gctaaattat cgtgggccat cggtccagat aagggagata tgatgaagca
     6781 ggcattacga gtagcatttg gttttctcat actgtgggca tcagttctgc atgctgaagt
     6841 ccgcattgtg atcgacagcg gtgtagattc cggtcgtcct attggtgttg ttcctttcca
     6901 gtgggcgggg cctggtgcgg cacctgaaga tattggcggc atcgttgctg ctgacttgcg
     6961 taacagcggt aaatttaatc cgttagatcg cgctcgtctg ccacagcagc cgggtagtgc
     7021 gcaggaagta caaccagctg catggtccgc actgggcatt gacgctgtag ttgtcggtca
     7081 ggtcactccg aatccggatg gttcttacaa tgttgcttat caacttgttg acactggcgg
     7141 cgcaccgggt actgtacttg ctcagaactc gtacaaagtg aacaagcagt ggctgcgtta
     7201 tgctggtcat accgccagtg atgaagtgtt tgaaaaactg accggcatta aaggtgcgtt
     7261 ccgtactcgt attgcctacg ttgttcagac caacggcggt cagttcccgt atgaactgcg
     7321 cgtatctgac tatgacggtt acaaccagtt tgtcgttcac cgttcaccgc agccgctgat
     7381 gtctccggcg tggtcaccag acggttctaa actggcttat gtgaccttcg aaagcggtcg
     7441 ttccgcgctg gttattcaga cgctggcaaa tggcgctgta cgtcaggtgg cttcattccc
     7501 gcgtcacaac ggtgcacctg cattctcgcc agacggcagc aaactggcat tcgccttgtc
     7561 gaaaaccggt agtctgaacc tgtacgtaat ggatttggct tctggtcaga tccgccaggt
     7621 gactgatggt cgcagtaaca ataccgaacc gacctggttc ccggatagcc agaacctggc
     7681 atttacttct gaccaggccg gtcgtccgca ggtttataaa gtgaatatca acggcggtgc
     7741 gccacaacgt attacctggg aaggttcgca gaaccaggat gcggatgtca gcagcgacgg
     7801 taaatttatg gtaatggtca gctccaatgg tgggcagcag cacattgcca aacaagatct
     7861 ggcaacggga ggcgtacaag ttctgtcgtc cacgttcctg gatgaaacgc caagtctggc
     7921 acctaacggc actatggtaa tctacagctc ttctcagggg atgggatccg tgctgaattt
     7981 ggtttctaca gatgggcgtt tcaaagcgcg tcttccggca actgatggac aggtcaaatt
     8041 ccctgcctgg tcgccgtatc tgtgataata attaattgaa tagtaaagga atcattgaaa
     8101 tgcaactgaa caaagtgctg aaagggctga tgattgctct gcctgttatg gcaattgcgg
     8161 catgttcttc caacaagaac gccagcaatg acggcagcga aggcatgctg ggtgccggca
     8221 ctggtatgga tgcgaacggc ggcaacggca acatgtcttc cgaagagcag gctcgtctgc
     8281 aaatgcaaca gctgcagcag aacaacatcg tttacttcga tctggacaag tacgatatcc
     8341 gttctgactt cgctcaaatg ctggatgcac atgcaaactt cctgcgtagc aacccgtctt
     8401 acaaagtcac cgtagaaggt cacgcggacg aacgtggtac tccggaatac aacatctccc
     8461 tgggtgaacg tcgtgcgaac gccgttaaga tgtacctgca gggtaaaggc gtttctgcag
     8521 accagatctc catcgtttct tacggtaaag aaaaacctgc agtactgggt catgacgaag
     8581 cggcatactc caaaaaccgt cgtgcggtac tggtttacta agagaattgc atgagcagta
     8641 acttcagaca tcaactattg agtctgtcgt tactggttgg tatagcggcc ccctgggccg
     8701 cttttgctca ggcaccaatc agtagtgtcg gctcaggctc ggtcgaagac cgcgtcactc
     8761 aacttgagcg tatttctaat gctcacagcc agcttttaac ccaactccag caacaacttt
     8821 ctgataatca atccgatatt gattccctgc gtggtcagat tcaggaaaat cagtatcaac
     8881 tgaatcaggt cgtggagcgg caaaagcaga tcctgttgca gatcgacagc ctcagcagcg
     8941 gtggtgcagc ggcgcaatca accagcagcg atcaaagcgg tgcggcggca tcaacgacgc
     9001 cgacagctga tgctggtact gcgaatgctg gcgcgccggt gaaaagcggc gatgcaaaca
     9061 cggattacaa tgcagctatt gcgctggtgc aggataaatc ccgccaggat gacgcaatgg
     9121 tggcatttca gaatttcatc aaaaattacc ctgattcaac ttacctgcca aacgccaatt
     9181 attggctggg tcagttaaac tacaacaagg gtaaaaaaga tgatgcggcg tactattttg
     9241 cttcggtagt gaaaaactat ccgaagtcac caaaggctgc tgatgcgatg tttaaagtcg
     9301 gcgtcatcat gcaggacaaa ggtgacaccg caaaagcgaa agccgtatac cagcaggtta
     9361 tcagtaaata ccctggtact gatggcgcta aacaggcaca aaaacgtctg aacgcgatgt
     9421 aatgcataac acacgaccag aagtcgcatt atttctggtc gtgtcgtgcg aatcataagc
     9481 agttgagtga tctgcatcga aatttttgtt gcgctcaagt ctgaaatcag taatatatgc
     9541 cgccgttgcc acgggatatc aaacaaaccg aaagcaacga aaaagtgggt cgttagctca
     9601 gttggtagag cagttgactt ttaatcaatt ggtcgcaggt tcgaatcctg cacgacccac
     9661 caatcgctaa ggtggaagcg gtagtaaaac gtgaaggata acgttgcatc agcaacggcc
     9721 cgaagggcga gacgaagtcg agtcatcctg cacgacccac cactaacata gttagttgta
     9781 gtatccagcg tagtattggg tgattagctc agctgggaga gcacctccct tacaaggagg
     9841 gggtcggcgg ttcgatcccg tcatcaccca ccaccgggtc gttagctcag ttggtagagc
     9901 agttgacttt taatcaattg gtcgcaggtt cgaatcctgc acgacccacc agttttagca
     9961 tcgaagacag atgttaagcg tgtaggataa cgttgcgtca gcaacggccc gtagggcgag
    10021 cgaagcgagt catcctgcac gacccaccac taattacggt gggttcggtg gaagtagttt
    10081 gtagtatcca gcgcagtatc gggtgattag ctcagctggg agagcacctc ccttacaagg
    10141 agggggtcgg cggttcgatc ccgtcatcac ccaccactcg ggtcgttagc tcagttggta
    10201 gagcagttga cttttaatca attggtcgca ggttcgaatc ctgcacgacc caccaatttt
    10261 aacatcaaac tcagatgtta agcgtgaagg ataacgttgc gccagcaacg gcccgtaggg
    10321 caagcgaagc gagtcatcct gcacgacaca ccaatcttaa agattggccc cgagtagaaa
    10381 tctttcaggt aacacccgta tgggtcgtta gctcagttgg tagagcagtt gacttttaat
    10441 caattggtcg caggttcgaa tcctgcacga cccaccatcc tgaatgatta aagcagcata
    10501 atcccgcaag gggtcgttag ctcagttggt agagcagttg acttttaatc aattggtcgc
    10561 aggttcgaat cctgcacgac ccaccaatgt aaaaaagcgc cctaaaggcg cttttttgct
    10621 atctgcgata ctcaaagatt cgaacctgca gcaggtttga gttgagcgca gcgaaacaac
    10681 ggagccgctc gcggcgacgg cccgaagggc gagcgaagcg agtcatcctg cacgacccac
    10741 caatgtaaaa aagcgcccta aaggcgcttt tttgctattc aggcatcctc aatttcactt
    10801 tgtaaacctg atgacatcgt cagagcttac tgtgcaagca actctacgtc ggtggaatta
    10861 ggcgtaattc gagtgacttt tccccaccat tcgactatct tgtttagcat ataaaacaaa
    10921 ttacaccgat aacagcgaat attacgctaa tgtcggtttt aacgttaagc ctgtaaaact
    10981 agatggtaag atgagcgtaa tgtttgatcc agacacggcg atttatcctt tccccccgaa
    11041 gccgacgccg ttaagcattg atgaaaaagc gtattaccgc gagaagataa aacgtctgct
    11101 aaaagaacgt aatgcggtga tggttgccca ctactatacc gatcccgaaa ttcaacaact
    11161 ggcagaagaa accggtggct gtatttctga ttctctggaa atggcgcgct tcggtgcaaa
    11221 gcatcccgct tctactttgt tagtcgctgg ggtgagattt atgggagaaa ccgccaaaat
    11281 tctcagtccg gaaaaaacaa ttctgatgcc gacacttcag gctgaatgtt cactggatct
    11341 cggctgccct gttgaagaat ttaacgcatt ttgcgatgcc catcccgatc gtactgtcgt
    11401 cgtctacgcc aacacttctg ctgcggtaaa agcgcgcgca gattgggtgg taacttcaag
    11461 catttccgtc gaacttattg atcatcttga tagtttgggt gaaaaaatca tctgggcacc
    11521 cgacaaacat ctggggcgtt acgtgcaaaa acagacgggt ggagacattc tatgctggca
    11581 gggtgcctgt attgtgcatg atgaatttaa gactcaggcg ttaacccgct tgcaagaaga
    11641 atacccggat gctgccatac tggtgcatcc agaatcacca caagctattg tcgatatggc
    11701 ggatgcggtc ggttccacca gtcaactgat cgctgctgcg aaagcattgc cacatcagcg
    11761 gcttatcgtg gcaaccgatc ggggcatttt ctacaaaatg cagcaggcag tgccagataa
    11821 agagttactg gaagcaccaa ccgcaggtga gggtgcaacc tgccgcagct gcgcgcattg
    11881 tccgtggatg gccatgaatg gccttcagtc catcgcagag gcattagaac aggaaggaag
    11941 caatcacgag gttcatgttg atgaaaggct gcgagagagg gcgctggtgc cgctcaatcg
    12001 tatgctggat tttgcggcta cactacgtgg ataacgaata ataaggcgta acgttacgct
    12061 ttgggggaaa gatggatttt tttagtgtgc agaatatcct ggtacatata ccaatagggg
    12121 caggcggtta tgatctctca tggatcgaag cggtaggcac gatcgccggg ttgctgtgta
    12181 ttggccttgc cagtctggag aagatcagca actacttctt tggcctgatc aacgtcacct
    12241 tgtttggcat tattttcttt cagattcagc tgtatgccag cctgctatta caggtgtttt
    12301 tctttgccgc gaatatttac ggttggtatg cgtggtcgcg acaaaccagt cagaacgagg
    12361 cggagttgaa aattcgctgg ttgccattgc cgaaggcact cagctggttg gcggtttgcg
    12421 ttgtttcgat tggtctgatg acggtattta tcaatccggt gtttgcattt ttgacccgcg
    12481 tggcagtcat gatcatgcaa gcattaggat tacaggttgt gatgcctgaa ctgcaaccgg
    12541 acgctttccc gttctgggat tcatgcatga tggtgttatc tatcgtggca atgattctga
    12601 tgacgcgtaa gtatgtggaa aactggctgt tgtgggtgat tattaacgtg attagcgtcg
    12661 ttatttttgc acttcagggc gtttacgcca tgtctctgga gtacatcatc ctgaccttta
    12721 ttgcgctcaa cggcagccgg atgtggatca acagcgcacg tgaaagaggc tcacgcgcgc
    12781 tgtcccatta atggtgatga tgtgaatggc cagacacccc ttcattcagg tggcagtctg
    12841 gcccatgaca aggttgatat tccatctgaa tggtggcgtg ctcaatctga tagtgatcca
    12901 tcaggtagtg ttggatctga tccaacaagg catcgtgatc gtgtggcggg atcacctgca
    12961 catgcaacgt catcaccggc ttctcgccta ccatccatac atgtacatgg tgcacattgc
    13021 ggacttccgg gatttcccgg cacatacgac gcttcagttc agcgatatcc agcgataccg
    13081 gtgcaccttc aagtaattca ttcacgctat ctttcaacag tcgccacgcg ctacgcagaa
    13141 ccagaagcga caccagtatc gagagaatgg ggtcagcagg tgtccagcca gtccagataa
    13201 taatcaacgc ggcaataata gccccaaccg aacccagcag gtccccgagc acatgcagtg
    13261 ccgctgctcg tacgttgagg tttttctctt cgctgccgtg atgaagtaac cagaaagaaa
    13321 gtatatttgc cagcaaccca gccacggcaa ttgccatcat catgccgccc tcgaccggac
    13381 gcggcgtgcg gaagcgttct atcgcctccc agacaatcaa aatggtaatt accacgaggg
    13441 ctatggcatt cacaaacgcc gcgagcgtgg tcagtcttag ccaaccgaaa gtgtggcgaa
    13501 tggtgggagg acgacgtgaa aattgcacgg cgagtagggc gaaaagcagg gcggcagtat
    13561 cggtcaacat atgacccgca tcggccagca atgccagaga accagaaaga aaaccaccaa
    13621 agacttctac cagcataaac ccggcagtca cgccgaaagc atacaacaag cgacgagcat
    13681 tattatcttc tggcaggtgt gaggacgtgt gtgagtgtga gtgcgccatg acgagtcatc
    13741 ccttaattat tctcacttaa ctttatgaca tcatactgct tttagaagtg aaaaattaaa
    13801 agggagagac tccgctctcc cattattggc tattttgcag ggttactgcg tggtaccgtc
    13861 ggttttggta tcgacatcat tattgatgcc atcaccggtt tgtacctttt tattgatatc
    13921 cggacagcga ccatctttgc acatggtgtt cttgtgctct tcatctttgg tcattccgtc
    13981 attgttcatt gaagaaccat ccgaatgcag cattgtgcca ccagaaccgg tatttacccc
    14041 gttattgtcg acgttatttg gcgcgacatt ttcacgggcg tcaggggcta cctggcccgc
    14101 atcagctgcg gcgtttgcct ggccgttatt agtttgcgct ccgctatcgg cagccagtgc
    14161 ggcaccgctg gcaaggctta gagtggcagt cagaaataat gtggccagtt ttgtcatttt
    14221 cataggatgc tcctgttatg gtcgttatgt cggataacct cttccaacag tgcatttgca
    14281 ggtgaatata aggcattggt ttaagatttc agccaggtta tgaaacgcag cagagaatct
    14341 tgaaataatt aacaaacaaa ggagttacag ttagaaattg taggagagat ctcgtttttc
    14401 gcgacaatct ggcgtttttc ttgctaattc caggattaat ccgttcatag tgtaaaaccc
    14461 cgtttacaca ttctgacgga agatatagat tggaagtatt gcattcacta agataagtat
    14521 ggcaacactg gaacagacat gaattatcag aacgacgatt tacgtatcaa agaaatcaaa
    14581 gagttacttc ctcctgtcgc attgctggaa aaattccccg ctactgaaaa tgccgcgaat
    14641 acggttgccc atgcccgaaa agcgatccat aagatcctga aaggtaatga tgatcgcctg
    14701 ttggtggtga ttggcccatg ctcaattcat gatcctgtcg cagcaaaaga gtatgccact
    14761 cgcttgctgg cgctgcgtga agagctgaaa gatgagctgg aaatcgtaat gcgcgtctat
    14821 tttgaaaagc cgcgtaccac ggtgggctgg aaagggctga ttaacgatcc gcatatggat
    14881 aatagcttcc agatcaacga cggtctgcgt atagcccgta aattgctgct tgatattaac
    14941 gacagcggtc tgccagcggc aggtgagttt ctcgatatga tcaccccaca atatctcgct
    15001 gacctgatga gctggggcgc aattggtgca cgtaccaccg aatcgcaggt gcaccgcgaa
    15061 ctggcgtctg gtctttcttg tccggtaggt tttaaaaatg gcacagacgg tacgattaaa
    15121 gtggctatcg atgccattaa tgccgccggt gcgccgcact gcttcctgtc cgtaactaaa
    15181 tgggggcatt cggcgattgt gaataccagc ggtaacggtg attgccatat cattctgcgc
    15241 ggcggtaaag agcctaacta cagcgcgaag cacgttgctg aagtgaaaga agggctgaac
    15301 aaagcaggcc tgccagcaca ggtgatgatc gatttcagcc atgctaactc gtccaaacaa
    15361 ttcaaaaagc agatggatgt ttgtgctgac gtttgccagc agattgccgg tggcgaaaag
    15421 gccattattg gcgtgatggt ggaaagccat ctggtggaag gcaatcagag cctcgatagc
    15481 ggggagccgc tggcctacgg taagagcatc accgatgcct gcattggctg ggaagatacc
    15541 gatgctctgt tacgtcaact ggcgaatgca gtaaaagcgc gtcgcgggta aggtttaatt
    15601 gtcggatgcg ccgtcagagt ggcgtatccg atgaatcacc acaggcctga taagtcgcgc
    15661 agcgtcgcat caggcaatgt gctccattgt tagcaacaaa aaagccgact cacttgcagt
    15721 cggctttctc attttaaacg aatgacgttt acttcgcttt accctggttt gcaaccgccg
    15781 ctgctttcgc tgcgatctcg tcagcattac ccagataata gcgtttcagc ggtttgaaat
    15841 tctcgtcgaa ctcatacacc agcggcacgc cagtcgggat attaagctca agaatctctt
    15901 cttcgctcat gttatcaaga tatttcacca gcgcacgtaa agagttaccg tgtgcagcga
    15961 tgatcacgcg ctcaccgctc ttcatacgcg gcagaatagt ttcattccag taagggatca
    16021 cgcggtcaat ggtcagcgcc aggctttccg tcagcggcag ttctttttcg ctcagtttcg
    16081 cgtaacgcgg atcgtgaccc ggataacgct catcatcttt agtcagttcc ggcggagtca
    16141 ctgcaaaacc acgacgccac tgtttcacct gctcgtcgcc atacttttca gcagtttccg
    16201 ctttgttcag accctgcaac gcaccgtagt gacgttcgtt cagtttccag gatttctcaa
    16261 cgggcagcca tgcctgatcc agttcgtcca gcacattcca cagggtatgg atagcgcgtt
    16321 tcagcacaga ggtgtaagca aagtcaaagc tgtaaccttc ctctttcagc agcttacctg
    16381 ctgcttttgc ttcgcttacg cctttctcag acagatccac gtcgtaccaa ccggtgaaac
    16441 ggttttcttt gttccactga ctttcgccat gacgaaccag aaccagctta gttacagcca
    16501 tatacttact cctcaaatca ttttttaatg ataataattc tcattatatt gccgtgacga
    16561 agcaacagca atgcttacgc ataaccatag cgaaaatagt gtcgcagtgt aaggttgttg
    16621 tgaatattga gttgcaaata tgtcggtgtt tgctggtgat ttgagcaata taaggataaa
    16681 gccctcatta cgagggcttc aggtgctact cagcaataaa ctgatattcc gtcaggctgg
    16741 aatactcttc gccaggacgc aggaagcagt ccggttgcgg ccattcaggg tggtttgggc
    16801 tgtccggcag aaactcgctt tccagtgcca ggccttgcca gtcggcgtaa ggttcagttc
    16861 ctcttgatgg cgtgccgccg aggaagttgc cggagtagaa ttgcagagcc ggagcggtgg
    16921 tgtagacctt cagctgcaat ttttcatctg ctgaccagac atgcgccgcc actttcttgc
    16981 catcgccttt ggcctgtaac aagaatgcgt gatcgtaacc tttcactttg cgctgatcgt
    17041 cgtcggcaag aaactcactg gcgatgattt tggcgctacg gaaatcaaaa gacgttccgg
    17101 cgacagattt caggccgtcg tgcggaatgc cgccttcatc aaccggcaga tattcgtccg
    17161 ccagaatctg caacttgtga ttgcgcacgt cagactgctc gccgtcaaga ttgaaataga
    17221 cgtgattagt catattcacc gggcaaggtt tatcaactgt ggcgcgataa gtaatagaga
    17281 tacggttatc gtcggtcaga cgatattgca ccgtcgcgcc gagattaccc gggaagccct
    17341 gatcgccatc atctgaactc agggcaaaca gcacctgacg atcgttctgg ttcacaatct
    17401 gccagcgacg tttgtcgaac ccttccgggc cgccgtgcag ctggttaacg ccctgacttg
    17461 gcgaaagcgt cacggtttca ccgtcaaagg tataacggct attggcgatg cggttggcat
    17521 aacgaccaat agaggccccc agaaacgcag cctgatcctg atagcattcc gggctggcac
    17581 agccgagcag cgcctcgcgg acgctgccat cggaaagcgg aatacgagct gaaagtaaag
    17641 tcgcacccca gtccatcagc gtgactacca tccctgcgtt gttacgcaaa gttaacagtc
    17701 ggtacggctg accatcgggt gccagtgcgg gagtttcgtt cagcactgtc ctgctccttg
    17761 tgatggttta caaacgtaaa aagtctcttt aatacctgtt tttgcttcat attgttcagc
    17821 gacagcttgc tgtacggcag gcaccagctc ttccgggatc agcgcgacga tacagccgcc
    17881 aaatccgccg ccggtcatgc gtacgccacc tttgtcgcca atcacagctt tgacgatttc
    17941 taccagagtg tcaatttgcg gcacggtgat ttcgaaatca tcgcgcatag aggcatgaga
    18001 ttccgccatc aactcgccca tacgtttcag gtcgccttgc tccagcgcgc tggcagcttc
    18061 aacggtgcgg gcgttttcag tcaggatatg acgcacgcgt ttcgccacga ttgggtccag
    18121 ctcatgtgca acagcgttga actcttcaat ggtgacatcg cgcagagctg gctgctggaa
    18181 gaaacgcgca ccggtttcgc actgttcacg acgggtgttg tattcgctgc caaccagggt
    18241 acgtttgaag ttactgttga tgatgacgac agccacacct ttcggcatgg aaactgcttt
    18301 ggtccccagt gaacggcagt caatcagcaa ggcatgatct ttcttgccga gcgcggaaat
    18361 tagctgatcc atgatcccgc agttacagcc aacaaactgg ttttctgctt cctgaccgtt
    18421 aagcgcgatt tgtgcgccgt ccagcggcag atgataaagc tgctgcaata cggttccgac
    18481 cgccacttcg agtgaggcgg aagaacttaa cccggcaccc tgcggcacat tgccgctgat
    18541 caccatgtcc acgccgccga agctgttgtt acgcagttgc agatgtttca ccacgccacg
    18601 aacgtagtta gcccattgat agttttcatg tgcgacaatg ggcgcatcga gggaaaactc
    18661 gtcgagctga ttttcataat cggctgccat cacgcgaact ttacggtcat cgcgtggtgc
    18721 acaactgatc acggtttgat aatcaatcgc gcagggcaga acgaaaccgt cgttgtagtc
    18781 ggtgtgttca ccaatcaaat tcacgcggcc aggcgcctga atggtgtgag tggcagggta
    18841 gccaaatgcg ttggcaaaca gagattgtgt tttttctttc agactcattt cttacactcc
    18901 ggattcgcga aaatggatat cgctgactgc gcgcaaacgc tctgctgcct gttctgcggt
    18961 caggtctcgc tgggtctctg ccagcatttc ataaccaacc ataaatttac gtacggtggc
    19021 ggagcgcaac agaggcggat aaaagtgcgc gtgcagctgc cagtgttgat tctcttcgcc
    19081 attaaatggt gcgccgtgcc agcccataga gtaggggaag gagcactgga agaggttgtc
    19141 ataacgactg gtcagctttt tcaacgccag cgccaaatcg ctgcgctggg cgtcggtcaa
    19201 atcggtgatc cgcaaaacgt gggctttggg cagcagtagc gtttcgaacg gccaggcagc
    19261 ccagtaaggc acaacggcta accagtgttc ggtttcgaca acggtacggc taccgtctgc
    19321 cagctcgcgc tgaacataat ccaccagcat tggtgatttc tgttcggcaa aatattcttt
    19381 ttgcaggcgg tcttcgcgct cagcttcgtt aggcaggaag ctatttgccc aaatctgtcc
    19441 gtgcggatgc gggttagagc agcccatcgc cgcacctttg ttttcaaaga cctgcaccca
    19501 cgggtacgtt tttcccagct ctgcggtttg ctcctgccag gttttgacga tttccgtcaa
    19561 tgccgcaacg ctcagttctg gcaacgtttt actgtgatcc ggtgaaaagc agatcacccg
    19621 gctggtaccg cgcgcgctct ggcaacgcat caaaggatcg ttgctttctg gcgcatctgg
    19681 cgtgtcagac atcaaggccg caaagtcatt agtgaaaacg taagtcccgg tgtaatcggg
    19741 gtttttatcg cctgtcaccc gcacattacc tgcgcagagg aagcaatctg gatcgtgcgc
    19801 aggtaacacc tgtttggctg gcgtttcctg cgccccctgc caggggcgct tagcgcggtg
    19861 cggtgaaacc agaatccatt gcccggtgag cgggttgtag cggcgatgtg gatgatcaac
    19921 gggattaaat tgcgtcatgg tcgttcctta atcgggatat ccctgtggat ggcgtgactg
    19981 ccagtgccag gtgtcctgcg ccatttcatc gagtgtgcgc gttacgcgcc agttcagttc
    20041 acggtcggct ttgctggcgt ccgcccagta ggccggaagg tcgccctcgc gacgcggtgc
    20101 aaaatgataa ttaaccggtt tgccgcaggc tttgctgaag gcattaacca cgtccagcac
    20161 gctgttgcct acgccagcgc cgaggttgta gatgtgtacg cctggcttgt tcgccagttt
    20221 ttccatcgcc acgacgtgac cgtccgccag atccattacg tggatgtaat cgcgtacgcc
    20281 agtaccatct tcggtcggat aatcgttacc aaaaatcgcc agcgagtcgc gacggcctac
    20341 agcaacctgg gcgatgtatg gcatcaggtt attcggaatg ccttgcggat cttcgcccat
    20401 atcgcccgac ggatgcgcgc caaccgggtt gaagtagcgc agcagggcaa tgctccagtc
    20461 cggctgggct ttttgcagat cggtgaggat ctgttccacc atcagcttgc ttttgccgta
    20521 agggctttgt ggtgtgccgg tcgggaagct ttcaacgtat ggaattttgg gctgatcgcc
    20581 ataaacggtg gcggaggagc taaaaataaa gtttttgacg ttagcggcgc gcatggcgct
    20641 aatcaggcgc agagtgccgt tgacattgtt gtcgtaatat tccagcggtt tttgtaccga
    20701 ttcgccaacg gctttcagcc cggcgaagtg gatcacggtg tcgatagcgt gatcgtgcag
    20761 tatctcggtc atcaacgctt cgttacgaat atcgccttca acaaacgttg gatgtttgcc
    20821 gcctaaacgc tcgataacag gcagtacgct gcgcttactg ttacagaggt tatcaagaat
    20881 gatgacatca tgaccgtttt gcagtaattg cacacaggta tgacttccaa tgtaaccgct
    20941 accaccggta accagaactc tcataattcg ctccattagg cttatggtat gaaataacca
    21001 tagcataaca aagatgcgaa aagtgtgaca tggaataaat tagtggaatc gtttacacaa
    21061 gaatttagcc gttttttatg cgcgattaag tgattataaa acagagggtt tatgaatgat
    21121 tgcgcttttt atctgaaaaa agaagcggtt tcatgcctgc atgcgtcgaa ccgttggccg
    21181 gagagggcgc ttaggccgcc tccggcaagg tcagcactac cgactcaata tatttttgtc
    21241 agcacatagc gatagagtcc accgtccggc acgaactcaa ggcgatgggt aatacaggca
    21301 ggcgcatctt cagcgtggtg cgaaacaaac aacaattgcg tttcaccttc gctaatcagc
    21361 acatcaacaa aacggcggat aagctggcga ttgagcggat caagcccctg tagtggttca
    21421 tcgagaataa gcaacgtcgg atgtttcacc agtgcgcgga cgatcagcgc cagacgctgc
    21481 tgtccccagg aaagactatg gaacggagcg tcagccgtac gtttatcaat gccgagaata
    21541 tccagccact gctgcaccag tttttgctgg cgatccgaaa cggcctgata aatgccaatc
    21601 gaatcaaaat agccagaaag aatcacatta cgcacagtag tactgacccg gtaatccaga
    21661 tgcaaactac tgctgacgta accgatatgc tttttgatat cccagatggt ttcgccgctg
    21721 ccgcgacgtc gtccgaaaag cgtcaaatcg ttgctgtaac cttgcggatg atcgccagta
    21781 accaggctta ataacgtcga ttttcctgca ccatttggcc cgacaatttg ccagtgttcg
    21841 cctggattca cctgccagct aaggttatta agaatggggc gatcgttata agaaaccacg
    21901 ccattgttca gcacaatgcg cggttcgttg gcgggtaagg cgtgacgtgc tgaaggttca
    21961 tccggctccg gcagttgcac accttcaagc tgttcactat gcgccagttg cgcgacgagt
    22021 gcttgttgga gcagttcctc tttagcgcca gtttccgcta acgtgcaatc cgccagcacg
    22081 ccagcaaact ggacaaactc cgggatctca tcgaagcgat tgagcaccag taccagagta
    22141 ataccggact gatgtaacga ggcgagtcgc tcagccagct gctgacgtga ggcaacatcc
    22201 aggccatcga acggctcatc aagaatcaac aagtcaggct ccgacatcag cgcctgacac
    22261 agcagggttt ttcgcgtctc gccagtggaa aggtatttaa agcgtcggtc gaggagggcg
    22321 gtaataccga actgctgcgc cagttgcatg caacgcggtg catcctttac ttcatcctga
    22381 atgatctcag ccgtagtgcg tccggtgtca tcttcgccag ggccgagcat atcggtgtta
    22441 ttccgctgcc attcgtcgct gacgagtttt tgcaattgct cgaaggagag acgagtgatg
    22501 tgggaaaact ggctttgccg ttcacctttc aaaagcggaa gttcccccgc cagcgcgcgg
    22561 gccagcgccg atttcccgct tccattcgaa ccgacaaacg cccaactatc acccgcgttt
    22621 aacgttagct gaggcaattg cagcgttttt gtgtcgctaa gacgaaacgc gccttgcaaa
    22681 atttgcaacg atgacatttt atatcccact ttgtgcagcg attactgaca gggatacgtg
    22741 tttcataaca aattgtcaac acgcttagca cagcgtggcg ataatcacgc tgtcggcatt
    22801 aaagtaggcc gtgacattct gtccttgctg aagagaagtc gcttcattta ccggcactgt
    22861 ggcgcacagt gtttgcccgt ccggtagcga cattattact tcgcactgct ctgcgccgcg
    22921 ctcaatatgg ctaataatac ccggtaattg gttgtcagcg ttttgcgcga ccgcttcgtc
    22981 ctgagtaatg cctacccacg gcgcttttag caatatcaac acttctttgc cttcatccag
    23041 ccccagacgc gcgccgcttt gtgcggtaat tgcgactttc aggcgtgttt ttccgtcagc
    23101 cagtaagaca tcaacatgct gttgaacgtc atcatgatcg cgggcggtga tggtaccgaa
    23161 ccactggtta cgggcgctgg tttgcagtga aaaacgtgag atcgcggcca acaggctgtt
    23221 cagcggcagg gcgtcatcgt cacttaacac atcaaaggct ttttgctgga tttgcgccag
    23281 taagtcatag agctgaatca gtcgctgacc atagcgggtc agtactgcgc cgccgccacc
    23341 tttaccgcct gttgcgcgct cgaccagaat atgctcactt aactgattca tctcgttaat
    23401 ggcatcccag gcgcttttat agctgatacc ggcatctttc gctccctggc taatggaacc
    23461 ggaaagcgca atgtgtttta gtagcgaaat gcggcgcggg tcggcgaata atttttgttg
    23521 gagcttaagg gtgagaagga tttcggcctg cataacaatg tcctggcaaa agtcttattg
    23581 tgacggaaaa cgaacgccac gcaaagctga ccgcacaaaa ggggagtgct tttctgtgct
    23641 tagcggttag aatagtctca tgactatatc tggagttgac catgttagag ttattaaaaa
    23701 gtctggtatt cgccgtaatt atggtacctg tcgtgatggc catcatcctg ggtctgattt
    23761 acggtcttgg tgaagtattc aacatctttt ctggtgttgt taaaaaagac cagcccggac
    23821 aaaatcattg attccctgaa tgcccgctta gtcgggcatt ttctttttct caacttcctg
    23881 cttttcctgc cgatattttt tcttatctac ctcacaaagg ttagcaataa ctgctgggaa
    23941 aattccgagt tagtcgttat attgtcgcct acataacgtt acattaaggg gttaccaatg
    24001 gctcgtaaat ggttgaactt gtttgccggg gcggcactct ctttcgctgt tgctggcaat
    24061 gcactggcag atgaagggaa aatcacggtg ttcgccgccg catcactgac taacgcaatg
    24121 caggacattg ctacgcagta taaaaaagag aaaggcgtgg atgtggtttc ttctttcgct
    24181 tcgtcatcta ctctcgcccg tcagattgaa gcgggtgccc ctgcggatct gtttatttct
    24241 gccgatcaga aatggatgga ttatgcggtt gataaaaaag cgatcgatac tgctacgcgt
    24301 cagacactgc tcggcaatag cctggtcgtt gtagcaccga aagccagcga gcagaaagat
    24361 ttcaccatcg acagcaaaac taactggact tcgctgctga atggcggtcg cctcgcggtt
    24421 ggcgatccgg aacatgtccc tgctggcatt tatgcaaaag aagcactgca aaaactgggc
    24481 gcatgggata cgctctcccc gaaactggct ccggcggaag atgttcgtgg cgcgctggcg
    24541 ttggtcgaac gcaatgaagc gccgttgggc attgtttacg gttctgacgc agttgccagc
    24601 aaaggggtaa aagtggttgc catcttcccg gaagattcac ataaaaaagt ggaatatccg
    24661 gttgctgttg tggaagggca taacaatgcg acagtgaaag cattttatga ttatctgaag
    24721 ggaccgcagg ctgcggaaat ctttaaacgt tacggattta caaccaagta atgatactga
    24781 ccgatccaga atggcaggca gttttattaa gcctgaaagt ttcttccctg gctgtgctgt
    24841 ttagcctgcc gtttgggatc ttttttgcct ggttactggt gcgttgcacg tttccgggca
    24901 aagctctgct cgacagcgta ctgcatctac cgctggtgtt accgcccgtg gtcgtcggtt
    24961 acttattatt agtttcgatg ggacggcgcg gatttatcgg tgaacgtctg tatgactggt
    25021 ttggtattac cttcgccttt agctggcgcg gcgcggttct cgctgccgcc gtcatgtcgt
    25081 ttccgctgat ggtgcgggca attcgtctgg cgctggaagg ggttgatgtc aaactggaac
    25141 aggccgcaag aacactgggg gccgggcgct ggcgcgtttt ctttactatc acgttaccgc
    25201 tgaccttacc gggaattatt gttggtacgg tactggcttt tgctcgttct ctcggtgagt
    25261 ttggtgcaac catcaccttt gtgtcgaaca ttcctggtga aacgcgaacc attccttctg
    25321 ccatgtatac cctgatccag acccccggcg gggaaagtgg agcggcgaga ctgtgcatta
    25381 tttctattgc gctggcgatg atctccctgt tgatttcaga atggctggcc agaatcagcc
    25441 gtgaacgggc ggggcgctaa tcatgctgga actgaatttt tcccagacgt tgggcaacca
    25501 ttgcctgact attaatgaaa cgctgcccgc caatggcatc actgctatct ttggcgtctc
    25561 cggtgccgga aaaacttcgc tgattaacgc catcagtgga ctgacgcgcc cgcaaaaagg
    25621 gcggattgtc ctcaatgggc gggtactaaa tgatgccgaa aaaggtatct gcctgacgcc
    25681 ggaaaagcgt cgcgttggct atgtttttca ggatgcgcgg ctgttcccgc attacaaagt
    25741 gcgtggcaat ctgcgctacg gcatgtcgaa aagtatggtc gatcagttcg ataagctggt
    25801 ggcgctttta ggcattgaac cgttgcttga ccgtttacca ggcagcctgt ccggaggcga
    25861 aaaacagcgc gtggcgattg gtcgggcttt gctgacagca ccggaattgc tgttgctgga
    25921 tgaaccgctg gcgtcactgg atattccgcg taaacgcgaa ctgttgcctt atctgcaacg
    25981 gctgacacgg gaaatcaaca ttccgatgtt gtatgtcagc cattcgctgg atgagatcct
    26041 ccatctggca gacagagtga tggtactgga aaacggtcag gtgaaagcct ttggcgcgct
    26101 ggaggaagtg tggggcagta gcgtgatgaa tccgtggctg ccgaaagagc aacaaagtag
    26161 cattctgaaa gtgacggtgc ttgagcatca tccgcattac gcgatgaccg cgctggcgct
    26221 gggcgatcag catttgtggg tcaataagct ggacgaaccg ctgcaagctg cgctacgtat
    26281 ccgcattcag gcttccgatg tttctctggt tttacaaccg ccgcagcaaa ccagcattcg
    26341 taacgtattg cgggcaaaag ttgttaatag ttatgacgac aacggccagg tggaagtgga
    26401 actggaagtc ggcggtaaaa cgctgtgggc gcgtatcagc ccgtgggcca gggatgaact
    26461 ggcgatcaaa cctggcctgt ggctgtacgc gcaaattaaa agtgtgtcga taaccgcctg
    26521 attaaatcag gtggctataa atgaactggg caatgctgtc ggtggtgtta tcaccaatca
    26581 caatgttggc gcgcgctttt accgcgtcat cggcgttgcc catcgccacg cctgtaccag
    26641 cggcttccag catactgata tcattaaagt tatcgccgaa tgccacgacg ttttccatcg
    26701 accaaccttg cgcctcaacc catttcgtca aacgtttacc tttgctgttg ccgccgcgtg
    26761 caatatcaac ctgatcgtgc caggaccatt cacactccag tcccagttca tgttcgacat
    26821 gcttaccaaa atgctgcaat tgcggcaggt catcgtgcgt cagggcgaac ttccatacgg
    26881 cgttaacttg ttgcgccgtt tcagccagag aagcgacttg tgtgaaagtc ggacgctgtt
    26941 ccggcggcag ggtttgcgcc cagttagacg tgcgaatgac atgcccggtc gggtgctcat
    27001 agaccattgc atcatccaca tacatcagac cgtgaatgtg gtgttcattc agcatctcaa
    27061 tgagttgcag ggctttatta acgggcattg ggtccgcttc cagcacggtt tttgcatgat
    27121 aatcatacaa ataggtgcca ttacagcaaa tagcaggtgt atccagcgcc agcgcctgat
    27181 aaaaaggatg aatagcgacg tgatggcgac ctgtgacgat gattaattga tagcctgctt
    27241 cgcgagcgcg ggccagggct tctatcgatg aaggaagcag ggtctttttc ggggtcaata
    27301 aggtgccgtc taagtcgaga gcaatcacgc gtgtggtcat ggcgtattcc agattaaggt
    27361 taagaatttt cgtctgcgcg aatggtacac cgataccact atcggacaaa attctgcgtt
    27421 ttaattcagc attcaccgcc aaaagcgact aattttagct gttacagtca gttgctaaat
    27481 gcaaaggagc attcatgaag caaacagttt atatcgccag ccctgagagc cagcaaattc
    27541 acgtctggaa cctgaatcat gaaggcgcac tgacgctgac acaggttgtc gatgtgccgg
    27601 ggcaggtgca gccgatggtg gtcagcccgg acaaacgtta tctctatgtt ggtgttcgcc
    27661 ctgagtttcg cgtcctggcg tatagtatcg ccccggacga tggcgcactg acctttgccg
    27721 cagagtctgc gctgccgggt agtccgacgc atatttccac cgatcaccag gggcagtttg
    27781 tctttgtagg ttcttacaat gcgggtaacg tgagcgtaac gcgtctggaa gatggcctgc
    27841 cagtgggcgt cgtcgatgtg gtcgaggggc tggacggttg ccattccgcc aatatctcac
    27901 cggacaaccg tacgctgtgg gttccggcat taaagcagga tcgcatttgc ctgtttacgg
    27961 tcagcgatga tggtcatctc gtggcgcagg accctgcgga agtgaccacc gttgaagggg
    28021 ccggcccgcg tcatatggta ttccatccaa acgaacaata tgcgtattgc gtcaatgagt
    28081 taaacagctc agtggatgtc tgggaactga aagatccgca cggtaatatc gaatgtgtcc
    28141 agacgctgga tatgatgccg gaaaacttct ccgacacccg ttgggcggct gatattcata
    28201 tcaccccgga tggtcgccat ttatacgcct gcgaccgtac cgccagcctg attaccgttt
    28261 tcagcgtttc ggaagatggc agcgtgttga gtaaagaagg cttccagcca acggaaaccc
    28321 agccgcgcgg cttcaatgtt gatcacagcg gcaagtatct gattgccgcc gggcaaaaat
    28381 ctcaccacat ctcggtatac gaaattgttg gcgagcaggg actactgcat gaaaaaggcc
    28441 gttatgcggt cgggcaggga ccaatgtggg tggtggttaa cgcacactaa ccgctgattt
    28501 aaccggcgca gtctctcctg cgccggtgta ttaacctatc tcctgtaacg catgtctctg
    28561 gcgttcgacg atattggtcc acaaattgtc tttatcgtca gtccacaaat taatcagcaa
    28621 ggcaaaaaag cgttctgctg ccggagaaag cacggcatct ttgcggcgaa taatccccaa
    28681 tgtccgacgt ataacgggtt ccaccagtgg aataccaata atcgaagaat agggcgcatg
    28741 gggcattgcc agaccaggaa gcgccgaaat ccccagtccc gcctctacca gtcctaatga
    28801 cgtcgaaaga tgacgcactt cgtaaaacca gtccagcttc cagggcttgt cggctagctg
    28861 ttgttctatc aacaatcggt tgccgctgga ggaacgtacg ccaatcattt tgtaacccac
    28921 taactcctgc cattctacaa gctgcttttt ggccaatgga tggtcacgcc gacaggccag
    28981 cacgaatggc tcgttgacca gtggggtaaa atcaatggat gaatttgtga cgttgttcat
    29041 gtttatgcca aagtctgatt cgttacacag caccgattcc atgcaattat tcgtgccttg
    29101 ttccagaatc cgcactttaa tattgggata cagctcatta aatttaccga ttgccagcgg
    29161 taaaaaataa aatactgcgg tcggaataca cgctaacgtc accataccac gatgataagc
    29221 attcatatca cgaatattaa aaagcgtttc atcgaatttc tttattaatt cccgcgcctc
    29281 cgggagcaac ctttttcctg cttttgttaa cgtaaccttg cgagttgtac gctcaaatag
    29341 ttgaatatgt aaatcttcct ccatcttttt tattcttcgc gttaatgcag gttgcgtaat
    29401 attgagtaat ttagcagcat tgttaaatga actggactct gccagtatga caaatgcctt
    29461 catacttgat aattcatgct tcattattac tccggaaaat ggaagcgacg attttgggtg
    29521 gctggtcgtt aaaaatttta actgcattta gccaacttaa attaatgaaa aaatgttatt
    29581 aatcgttgag ctaaagtcat tagagatgct ttgtccttaa tgtaaccata tcgcaataag
    29641 ttatgttttt aaattgaggg cattattatg aaaaaaatac cctgcgtgat gatgcgaggt
    29701 ggaacctcga ggggcgcgtt cctgttagcg gaacatttac ccgaagatca aacgcagcgc
    29761 gataaaatat tgatggcaat tatgggttcc ggtaacgatc tggaaattga cggtattggc
    29821 ggcggtaatc cactgaccag taaagtcgcc attattagcc gttccagcga tccgcgtgct
    29881 gatgttgatt atctgtttgc tcaagttatc gtccatgagc aacgtgtcga taccacgcct
    29941 aactgcggca atatgctttc tggcgttggg gcattcgcca ttgaaaatgg tttgattgca
    30001 gcgacatcac cggtcacccg tgtgcgtatc cgcaacgtca atacgggtac gttcatcgaa
    30061 gctgatgtgc aaacgccaaa tggtgttgtc gagtacgagg gtagtgccag aattgacggc
    30121 gtaccgggta ctgccgcacc ggttgcgctc actttcctga atgccgctgg aaccaaaacc
    30181 ggaaaagttt tcccgactga taatcagatt gattattttg acgatgtccc ggtgacctgt
    30241 atcgatatgg cgatgccagt cgtcattatt ccggctgaat atctgggaaa aacaggttat
    30301 gaattaccgg cggaactgga tgccgacaaa gcattattag cccgcattga atctatccgt
    30361 ctacaagcgg gtaaagcaat gggcttaggt gatgtcagta atatggttat ccctaaacct
    30421 gtgcttattt ctccagcgca gaaaggcggg gcaattaatg tgcgttattt tatgccgcat
    30481 tcttgccatc gcgcgctggc gataaccggt gctattgcta tttccagtag ttgtgcattg
    30541 gaaggcaccg tcacccgaca aatcgtccct tctgtaggat acggcaatat caatattgaa
    30601 caccccagtg gtgcgctcga cgttcattta agtaatgaag gtcaggatgc cacgacgtta
    30661 cgcgcatctg ttattcggac gaccagaaaa atattttccg gtgaagttta tcttccctga
    30721 aaaaattcgt tgtcaagata aggataatca ataaaggact tctgtatgag tcatacagaa
    30781 agaacaggat tttaaatgaa taagaaatcg ttatggaagc taattctgat attagcgatc
    30841 ccatgtatta ttggttttat gccagctccg gcaggattaa gcgaactggc gtgggtgctt
    30901 tttggtattt acctggcggc cattgtgggg ctggttatca agcctttccc ggaacctgtc
    30961 gtactgttaa ttgccgttgc tgcctccatg gtggtggtcg gtaacttatc cgacggtgcg
    31021 tttaagacca ccgccgtatt aagcggttac tcttcaggta ctacctggct ggtgttctcg
    31081 gcgtttacct taagcgccgc atttgtgacc accggtttag gtaaacgtat tgcctatctg
    31141 ctgattggta aaatcggtaa caccacgctg ggtctgggtt acgttacggt attcctcgat
    31201 ctggtactgg ctccggcaac accgtctaac accgcgcgtg cgggcggtat tgtgttaccg
    31261 atcatcaaca gcgtggcggt ggctttgggg tccgaaccgg aaaaaagtcc gcgtcgtgtc
    31321 ggacattacc tgatgatgtc catttacatg gtcaccaaaa ccaccagcta tatgttcttt
    31381 accgcaatgg cggggaacat tctggcgctg aaaatgatca acgacattct gcacctgcaa
    31441 attagctggg gtggatgggc actggccgcc ggattgccgg gcatcattat gctgctggtc
    31501 accccactgg tgatttatac catgtatccg ccagaaatta agaaggtgga taataaaacc
    31561 atcgctaaag cgggccttgc cgaactggga ccgatgaaaa tccgcgaaaa aatgctgctc
    31621 ggtgtctttg tgctggcgct actgggctgg attttcagta agtctctggg ggttgatgaa
    31681 tccactgtgg caatcgttgt tatggcgacc atgctgctgc tgggtatcgt tacctgggaa
    31741 gacgtggtta aaaataaagg cggctggaat accttaatct ggtacggcgg tattatcgga
    31801 ttaagctcct tattatcgaa agttaaattc ttcgaatggt tagctgaagt ctttaaaaat
    31861 aacctggcat ttgatggtca cggtaacgtt gctttcttcg ttattatttt cctcagcatt
    31921 atcgtgcgtt atttcttcgc ttccggtagt gcctatatcg ttgctatgtt accggtattt
    31981 gccatgctgg cgaacgtctc cggcgcaccg ttaatgttaa ccgcgctggc actgttgttc
    32041 tccaactcct atggcggcat ggttactcac tatggcggcg cggcaggtcc ggtcatcttt
    32101 ggcgtgggtt ataacgatat taaatcctgg tggttggtcg gtgcggtact gacgatatta
    32161 accttcctgg tgcatatcac cctcggcgtg tggtggtgga atatgctgat cggctggaac
    32221 atgctgtaaa tattctcgtc atacttcaag ttgcatgtgc tgcgtctgcg ttcgctcacc
    32281 ccagtcactt acttatgtaa gctcctgggg attcactcgc ttgtcgcctt cctgcaactc
    32341 gaattattta gagtatatcc atttattatc tttctgcgca cttcacggtg cgcagatatc
    32401 tggagcattt gatgatcaag ttatctgaaa aaggcgtgtt tctcgccagt aataacgaaa
    32461 taattgccga agaacatttc accggcgaaa ttaaaaaaga agaagccaaa aaaggcacta
    32521 ttgcctggtc tattctctct tcgcataata cgtccggaaa tatggataaa cttaaaatta
    32581 agtttgattc attagcctct cacgatatta cctttgttgg tattgtacag accgctaaag
    32641 cgtccggtat ggaacgtttc ccgctgccgt atgtgctgac caactgccat aactcactct
    32701 gcgccgtcgg cggcactatt aacggtgatg accatgtttt tggtttatcg gcggcccagc
    32761 gttatggcgg tatttttgtg cctccgcata ttgcggtcat ccatcaatat atgcgtgaga
    32821 tgatggcagg cggcggcaaa atgatcctcg ggtcagacag ccacacccgt tacggtgcat
    32881 tagggacaat ggcagtcggt gagggcggcg gtgagttggt aaaacagctg cttaatgaca
    32941 cctgggatat cgactatccg ggcgtggttg cggtgcatct gaccggaaaa cctgcgccgt
    33001 atgtggggcc acaggatgtg gcgctggcta tcattggcgc ggtgttcaaa aacggttacg
    33061 tcaaaaacaa agtcatggag ttcgttggac cgggcgttag cgcgctctct accgatttcc
    33121 gtaacagcgt tgacgtgatg accacggaaa ccacctgttt aagctccgtc tggcaaactg
    33181 atgaagaagt gcataactgg ctggcgctgc acggtcgcgg ccaggattac tgccagctta
    33241 accctcaacc gatggcgtac tacgatggct gcatcagcgt tgatttaagc gccatcaaac
    33301 caatgattgc gctgccgttc cacccgagca acgtgtataa aatcgacaca ctgaaccaga
    33361 acctgaccga cattctgcgt gagattgaaa ttgagtccga acgcgtggcg cacggtaaag
    33421 ccaaactctc gctgctggat aaagtggaaa atggtcgcct gaaagtgcag caggggatta
    33481 tcgcgggctg ttctggcggt aactacgaaa acgtcatcgc ggcggcgaat gcactgcgcg
    33541 gtcaatcctg tggcaatgac accttctcgc tggcagttta cccgtcatca cagccggtgt
    33601 ttatggatct cgccaaaaaa ggtgtggtag cagatttgat tggcgcaggc gcaatcatca
    33661 gaaccgcgtt ctgcggccca tgctttggcg cgggcgatac gccaatcaac aacggtttga
    33721 gtattcgcca caccacgcgt aacttcccga accgcgaagg ctctaagcca gctaatgggc
    33781 agatgtcagc ggtggcgttg atggacgctc gttctatcgc tgcgactgcg gcaaacggtg
    33841 gctatttaac ctctgccagc gaacttgatt gctgggacaa cgtgccggag tacgccttcg
    33901 atgtaacgcc gtataaaaac cgtgtttatc agggctttgt gaaaggggca actcagcaac
    33961 cgctgattta cggaccgaac attaaagact ggccggaatt gggtgcgctg actgacaata
    34021 tcgtcctgaa agtgtgctcg aagatcctcg acgaagtgac caccaccgac gaactgattc
    34081 cttccggtga aacctcttct tatcgttcaa atccgattgg tctggcggag tttaccctgt
    34141 cacgccgcga tcccggttat gttggcagaa gtaaagcgac tgctgagctg gaaaatcagc
    34201 gtctggcggg gaatgtcagc gagctgacag aggtgtttgc gcgcattaag cagattgctg
    34261 gtcaggagca tattgatccg ctgcaaactg aaattggcag catggtatat gcggtgaaac
    34321 caggcgatgg ttctgcgcgt gaacaggcgg cgagctgcca gcgtgtgatt ggcggtctgg
    34381 cgaatattgc cgaagagtac gcgactaaac gctaccgttc taacgtcatc aactggggga
    34441 tgttaccgct gcagatggcg gaagtgccaa cctttgaagt gggggattac atttacatcc
    34501 ctggcattaa agcggcgctg gataatccgg gtacgacgtt taaaggttat gtgatccatg
    34561 aagatgcgcc ggtaacggaa attacgctct atatggaaag tctcactgct gaagagcgcg
    34621 agattatcaa ggcgggtagt ttgattaact tcaataaaaa ccgtcagatg taaaaagcgc
    34681 catgtgaatg taggtcgcat tcggcactta ttgtcggatg cgatgcttgc gcatcttatc
    34741 cgacctacga atcgcatcga atctgtaggc cagataaggc attttcgcag catccggcac
    34801 ttattgtcgg atgcgatgct tgcgcatctt atccgaccta caaatcgcat cgaaccgtag
    34861 gccggataag gcgtttacgc cgcatccggc aaatagttaa ttgctcttac ttcttcgcct
    34921 ctgcaaccac tttactaccc acgccgcggt tattgtattc ccacatgcgg ttgtagttag
    34981 tgtcattcag attgcgctgt atttcgtcgt tatcatctac gctgccggta ttacccgcaa
    35041 acggacgatt agagatcacc gcatcggccc acggtttagc cgtgttaaaa ccttcgttga
    35101 tggcgctatc acggatcacc acctgaccgt tggtattggc atcaacatcc agcgagcggc
    35161 ccagttgcgc cacaccatca ccgaaagcat tgaaacggct gtttacggcg aggaaaccgt
    35221 agtaaatgtt ggacagcgta gccggtgcaa acacatacgc ttcttgctga gtacgtgagt
    35281 tcaccacgcg gaattcggtg ttatcgaaca ccactgcgcc gcgaccagaa acgatatcca
    35341 catccccttc aatgtagctg ttggtcacca gcgtacgcgg ctgacgattc gtttccagac
    35401 ggttctgcac accgctgttg gtgacaaaga aggtgttctg acgaccgaga atgttaacgt
    35461 tgttaatctg tacctggtca ccatcagtac gcagtgccac cgccggatgg ttacctgcat
    35521 ctacgctatc gcccagcgtg ttttcgatgg tcagattttg cagttgcagg ccattgtttt
    35581 gtgaccagaa gaccgcagag cagagaacac cgatactgtc gctgcgtttg ctctggcagc
    35641 tatcgtacat ataccacgct ggtttacctg gcatatattt gccgcgcggg ttgacgtcgt
    35701 gacgccagtc ggcagggctc atgccaccat caagggaaag cccaatcttc acatcaatcg
    35761 gtttttcacc tgtaccgtac agagtaattc cacccggagc ggcagggaca tataccgttc
    35821 cctgatactc accaggcatc acggcaatat actggcgctt gttggtacgc ttgataattg
    35881 ccgcatctac cgccgcctga atcgtggtat gcgttacacc ttgagtgccc gccgggccga
    35941 caacaaagtc aggttgcgca ggcagggtaa tcggggaagg attccacgct gctgcacctg
    36001 gtgtcaggga tgcaaaatag tgttgagcat cgaaattctg cgcttctttt gccgacagaa
    36061 tcgggcgaga agaggtacca ggcgcggttt gatcagaagg acgttgatcg ggcggggttg
    36121 agctacaggc ggtcagcgtc acgccaaaag ccaatgccag cgccagacgg gaaactgaaa
    36181 atgtgttcac aggttgctcc gggctatgaa atagaaaaat gaatccgttg aagcctgctt
    36241 ttttatacta agttggcatt ataaaaaagc attgcttatc aatttgttgc aacgaacagg
    36301 tcactatcag tcaaaataaa atcattattt gatttcaatt ttgtcccact ccctgcctct
    36361 gtcatcacga tactgtgatg ccatggtgtc cgacttatgc ccgagaagat gttgagcaaa
    36421 cttatcgctt atctgcttct catagagtct tgcagacaaa ctgcgcaact cgtgaaaggt
    36481 aggcggatcc ccttcgaagg aaagacctga tgcttttcgt gcgcgcataa aataccttga
    36541 tactgtgccg gatgaaagcg gttcgcgacg agtagatgca attatggttt ctccgccaag
    36601 aatctctttg catttatcaa gtgtttcctt cattgatatt ccgagagcat caacatgcaa
    36661 tgctgttggg atggcaattt ttacgcctgt tttgctttgc tcgacataaa gatatccatc
    36721 tacgatatca gaccacttca tttcgcataa atcaccaact cgttgcccgg taacaacagc
    36781 cagttccatt gcaagtctga gccaacatgg tgatgattct gctgcttgat aaattttcag
    36841 gtattcgtca gccgtaagtc ttgatctcct tacctctgat tttgctgcgc gagtggcagc
    36901 gacagggttt gttgttatat ggccttcagc tattgcctct cggaatgcat cgctcagtgt
    36961 tgatctgatt aacttggctg acgccgcctt gccctcgtct atgtatccat tgagcattgc
    37021 cgcaatttct tttgtggtga tgtcttcaag tggagcatca ggcagacccc tccttattgc
    37081 tttaattttg ctcatgtaat ttatgagtgt cttctgcttg attcctctgc tagccaggat
    37141 tttttcgtag cgatcaagcc atgaatgtaa cgtaacggaa ttatcactgt tgattctcgc
    37201 tgtcagaggc ttgtgtttgt gtcctgaaaa taactcaatg ttggcctgta tagcttcagt
    37261 gattgcgatt cgcctgtctc ggcctaatcc aaactcttta cccgtccttg ggtccctgta
    37321 gcagtaatat ccattgtttc ttatataaag gttagggggt aaatcccggc gctcatgact
    37381 tcgccttctt cccatttctg atcctcttca aaaggctacc tgttactggt cgatttaagt
    37441 caacctttac cgctgattcg tggaacagat actctcttcc atccttaacc ggaggaggga
    37501 atatcctgca ctcgcgtacc catcgacgaa ctgtttcaag gcttcttggg cgtcgctggc
    37561 gtgcgttcca ctcctgaagt gtcaagtaca tcgcaaagtc tccgcaatta cacgcaagaa
    37621 aaaaccgcca tcaggcggct tggtgttctt tcagttcttc aattcgaata ttggttatgt
    37681 ctgcatgtgc tatctgcgcc catagcatcc agtggtcata gcagtcattg atgttctctg
    37741 cttcgataac tctgttgaat ggctctccat tccattcacc tgtgactcga aagtgcattt
    37801 atcatctcca taaaacaaaa cccgccgtag cgagttcaga taaaagaaat ccccgcgagt
    37861 gcgaggatag ttacttgttc atattattaa tcgtcaatgt attttgagca ttgtgggcaa
    37921 tcatcaatcc cacaatacga ttcatatgca tcctttattg cgtcgcgggc ttcagtaaga
    37981 gtattgaata agttgcagct attatctttt tgatataggt aagttcctaa tttataagca
    38041 gaagaagcat catttccgct gtctaaaatt acatcgttat ggattctgca ccttgcaaga
    38101 actcctgatc ccataagggt ctgcatagcc cattgctctt gatcttcaca caaatcatga
    38161 atgctcattt caacacctct cttcacgttt cacacacgtt aagattaaca gtgtttttac
    38221 atgctttgga agatttattt tataaaaact cttttaatac aaatagatat aatagttcac
    38281 tattatagct cctttaatcg aggcggttct ggtagaggca tccagtgggt tacctctttg
    38341 agatacaggt cttcgccatc accgtcatcc caagtgggat tgccatcatt aaaccagtcg
    38401 ccatatacgc cgacctgagt gttggggatg tttggtgggt agttgttttt aaagtcagct
    38461 gctaacacat agcattgtcg ctctcccatt tctggcattc gctcactaca gcttatccaa
    38521 ccatccggag ttaccggata gttgccgggt tctttaatgt gcaagcgagg ctcaccatct
    38581 tttggctcag gccactggcg ctccatgttg atcttcaatt tatcttccat agcagcggta
    38641 atttcagcat cgctgatgcc agcacggcgc tgtgcatccc acaacagaaa ctgcatatca
    38701 gcccactcgc taagatcgtc tggttcggct gcggcttcca gtgcctcttt tgagagatgt
    38761 ttcagcggac caatggggcc aacgcagcca aatgtggagt cagaccattt ggcatgctcg
    38821 tggcgaatct gttcgcgttc cagtgatgcc agtgcaattc gtgccagttc catttgttcg
    38881 ccacgagtaa gtccgttatc aagcggattt ttaatgaata atttgatacg ttctttggtt
    38941 atagcgctca tatcactctc ctttgatgcg aatgcctgtt gcaatgctgt ttatgatgct
    39001 gtcagtgcat ggggtagaaa gctgggcatc tccagcaatt ctcatgacct caacatctgc
    39061 atatcgaata ccgaggtgta tcagaccggc tatacctgac ttaagccgag cattttccat
    39121 aaacagatcc tttgcccgct gtttttctgc ctcaagctca acgcgcaact tccctaccgt
    39181 taacgcaata tcctcgttct cttggtcgcg gcgtttgatg tattgctggt ttctttcccg
    39241 ttcatccagc agtgccagca caacctgagg tgtgactttc atacgaaatg ccagcaattt
    39301 ttgaggcgtt gctactgttt caattgctac tgccgcctca cgcagtgcct gagagttaat
    39361 ttcgctcact tcgaacctct ctgtttactg ataagctcca gatcctcctg gcaacttgca
    39421 caagtccgac aaccctgaac gaccaggcgt cttcgttcat ctatgggatc gccacactca
    39481 caacaatgag tggcagatat agcctggtgg ttcaggcggc gcatttttat tgctgtgttg
    39541 cgctgtaatt cttcaatttc tgatgctgaa tcaatgatgt ctgccatctt ccattaatcc
    39601 ctgaattgtt ggttaatacg cttgagggtg aatgcgaaca ataaaaaagg agcctgtagc
    39661 tccctgatga ttttgctttt catgttcatc gctccttaaa gacgccgttt aacatgccga
    39721 tcgccagact taaatgagtc ggtgtgaatc ccatcagcgt taccgtttcg cggtgcttct
    39781 tcagtacgct acggcaaatg tcatcgacgt ttttatccgg aaactgctgt ctggcttttt
    39841 tgatttcaga gttagccaga cgggcaatgc tgcgaagggc gttttcttgc tgaggtgtca
    39901 ttgaacaagt cccatgtcgg caagcataag cacacaaaat atgaagcccg ctgccagaaa
    39961 aatgcattcc gtggttgtca tacctggtct ctctcatctg cttctgcttt cgccaccatc
    40021 atttccagct tttgtgaaag ggatgcggct aacgtatgaa attcttcgtc tgtttctact
    40081 ggtattggca caaacctgac tccaatttga gcgaggctat gtgccatctc aatgctcgtt
    40141 cttaactcaa caggagatgc tttgtgcata cagcccctcg tttattattt atctcctcag
    40201 ccagccgctg tgctttcaga ggatttcgga taacaggaag gccgggaaat acccagcctc
    40261 gctttgtaac ggagtagacg aaagtgatcg cgcctacccg gatattatcg tgaggatgct
    40321 tcatcgccat tgctccccaa atacaaaacc aatttcagcc agtgccacgt ccattttttc
    40381 gatgaactcc ggcaccatct cgtcaaaatt cgccatgtac ttttcattcc gctcaatcac
    40441 gacataatgc aggccttcac gcttcatacg cgggtcatag ttggcaaagt accaggcatc
    40501 ttttcgcgtc acccacatgc tgtactgcac ctgggccatg taagccgact ttatggcctc
    40561 gaaaccaccg agccggaact tcatgaaatc ccgggaggta aacgggcatt tcagctcaag
    40621 gccgttgccg tcactgcata aaccatccgg agagcaggcg gtacgcatac tttcgtcgcg
    40681 atagatgatc ggggattcag taacattcac gccggaagta aactcaaaca gggctctggc
    40741 gtcgttctcg tactgttttc cccaggccag cgccttagcg ttaacttccg gagccacacc
    40801 ggtgcaaacc tcggcaagca gggtgtggaa gtaggacatt ttcatgtcag gccatttctt
    40861 tccggagcgg ggttttgcta tcacattgtg aacctctgaa gcggtgatga cgccgagccg
    40921 taatttgtgc cacgcatcat ccccctgttc gacagctctc acgtcgatcc cggtacgctg
    40981 cagtataatg tccggtgtca tgctgccacc ttctgctcag tggctttctg tttcaggaat
    41041 ccaagagctt tcactgcttc ggcctgtgtc agttctgacg atgcgcgaat gtcgcggcga
    41101 aatatctggg aacagagcgg caataagtcg tcatcccatg ttttatccag ggcaatcagc
    41161 agagtgttaa tctcctgcat ggtttcatcg ttaaccggag tgatgtcgcg ttccggctga
    41221 cgttctgcag tgtatgcggt attttcgaca atgcgctcgg cttcatcctt gtcatagata
    41281 ccagcaaatc cgaaggccag acgggcacac tgaatcatgg ctttatgacg taacatccgt
    41341 ttgggatgcg actgccacgg ccccgtgatt tctctgcctt cgcgggtttt gaatggttcg
    41401 cggcggcatt catccatcca ctcggtaacg cagatcggat gattacggtc cttgcggtaa
    41461 atccggcatg tgcaggattc attgtcctgc tcaaagtcca tgccatcaaa ctgctggttt
    41521 tcattgatga tgcgggacca gccatcaacg cccaccaccg gaacgatgcc gttctgctta
    41581 tcagggaagg cgtaaatttc tttcgtccac ggattaaggc cgtactggtt ggcgacgatc
    41641 aacaatgcga tgaactgcgc atcgctggca tcacctttaa atgccgtctg acgaagagtg
    41701 gtgatcagtt cctgtgggtc gacagaatcc atgccgacac gttcagccag cttcccagcc
    41761 agcgttgcga gtgctgtact catccgtttt atacctctga atcaatatca acctgatggt
    41821 gagcaatggt ttcaaccatg aaccggatgt gttctgccat gcgctcctga aactcaacat
    41881 cgtcatcaaa tgcacgggta atggcttttt tgctggcccc gcagcgttgt aaatgatcga
    41941 tgcagagcga ttcaaacaaa tgctggggca ggcctttttc catgtcgtct gccagttctg
    42001 cctctttctc ttcacgggcg agctgctggt agtgacgcgc ccagctctga gcctcaagac
    42061 gatcctgaat gtaataagcg ttcatggctg aactcctgaa atagctgtga aaatatcgcc
    42121 cgcgaaatgc cgggctgatt aggaaaacag gaaagggggt tagtgaatgc ttttgcttga
    42181 tctcagtttc agtattaata tccatttttt ataagcgtcg acggcctcac gaaacatctt
    42241 ttcatcgcca ataaaagtgg cgatagtgaa tttagtctgg atagccataa gtgtttgatc
    42301 cattttttgg gactcctggc tgattaagta tgtcgataag gcgtttccat ccgtcacgta
    42361 atttacgggt aattcgttca agtaaagatt cggaagggca gccagcaaca ggccaccctg
    42421 caatggcata ttgcatggtg tgctccttat ttatacataa cgaaaaacgc ctcgagtgaa
    42481 gcgttattgg tatgcggtaa cgcagcgctc aggcggcttt gatagtcata tcatctgaat
    42541 caaatattcc tgatgtatcg atatcggtaa ttcttattcc ttcgctacca tccattggag
    42601 gccatccttc ctgaccattt ccatcattcc agtcgaactc acacacaaca ccatatgcat
    42661 ttaagtcgct tgaaattgct ataagcagag catgttgcgc cagcatgatt aatacagcat
    42721 ttaataaaga gccgtgttta ttgagtcggt attcagagtc tgaccagaaa ttattaatct
    42781 ggtgaagttt ttcctctgtc attacgtcat ggtcgatttc aatttctatt gatgctttcc
    42841 agtcgtaatc aatgatgtat tttttgatgt ttgacatctg ttcatatcct cacagataaa
    42901 aaatcgccct cacattggag ggcaaagaag atttccaata atcagaacaa gtcggctcct
    42961 gtttagttac gagcgacatt gctccgtgta ttcactcgtt ggaatgaata cacagtgcag
    43021 tgtttattct gttatttatg ccaaaaataa aggccgacta tgcggcctga aattacttaa
    43081 ccaatgatgc tgcatattcg ataaggtaaa gctttggggc cagccaaatt tttaaccaag
    43141 tcatattggt tactacacca ataataaaaa tcccccacag agtcaaaact ccaaccaatg
    43201 gcatgataag aagattaata tcacctttgc tatcccaaac cattgtcggc ctgtatttgg
    43261 gatttccttt ctcccatgaa tatccttcat caccgatttt acctgtctca actctttggc
    43321 actgcttctt cataaaccag aaaaccagtg ggattgttag aatggctatt aatgttttaa
    43381 tcagactgtc aaccatattc catagcagca actgatgaac aacatcagga atctgtgcct
    43441 ggctaaatga aacagcagca tctattccat tgctggcttt ttgcagtagt tctacgagaa
    43501 tcttgtttgc ttgttcttcc atatatcacc ttgattgtaa taagcatgaa attatttacg
    43561 gacaaaaata aaggccacca tcaggcagcc ttgtagttct gtttaccaag ttctctggca
    43621 atcattgtcg tcgttcgtat tgtccacacc attgattctt atcaatagtc gtagtcatac
    43681 ggatagtcct ggtactgttc cattacatcc tgcggatgct cttcgaactc ttcaaattct
    43741 tcttccatat atcaccttaa atagtggatt gcggtagtaa agattgtgcc tgtcttttaa
    43801 ccacatcagg ctcggtggtt ctcgtgtacc cctacagcga gaaatcggat aaactattac
    43861 aacccctaca gtttgatgag tatagaaatg gatccactcg ttattctcgg acgagtgttc
    43921 agtaatgaac ctctggagag aaccatgtat atgatcgtta tctgggttgg acttctgctt
    43981 ttaagcccag ataactggcc tgaatatgtt aatgagagaa tcggtattcc tcatgtgtgg
    44041 catgttttcg tctttgctct tgcattttcg ctagcaatta atgtgcatcg attatcagct
    44101 attgccagcg ccagatataa gcgatttaag ctaagaaaac gcattaagat gcaaaacgat
    44161 aaagtgcgat cggtaattca aaaccttaca gaagagcaat ctatggtttt gtgcgcagcc
    44221 cttaatgaag gcaggaagta tgtggttaca tcaaaacaat tcccatacat tagtgagttg
    44281 attgagcttg gtgtgttgaa caaaactttt tcccgatgga acgggaagca tatattattc
    44341 cctattgagg atatttactg gactgaatta gttgccagct atgatccata taatattgag
    44401 ataaagccaa ggccaatatc taagtaacta ggtaagagga atcgattttc ccttaatttt
    44461 ctggcgtcca ctgcatgtta tgccgcgttc gccaggcttg ctgtaccatg tgcgctgatt
    44521 cttgcgctca atacgttgca ggttgctttc aatctgttcg tggtattcag ccagcactgt
    44581 aaggtctatc ggattcagtg cgctttctac tcgtgatttc ggtttgcgat tcagcgagag
    44641 aatagggcgg ttaactggtt ttgcgcttac cccaaccaac aggggatttg ctgctttcca
    44701 ttgagcctgt ttctctgcgc gacgttcgcg gcggcgtgtt tgtgcatcca tctggattct
    44761 cctgtcagtt agctttgagt aacgcgccgt gatgcttatc cccacggttg ctgtcttgca
    44821 gctgcatttc gcgctactca aagctttctg ctttgaatgc tgcccttctt cagggctaaa
    44881 tttttaagag cctcaccttc aatggtggtt agtgcgtcct gctgacgagc taagtatcac
    44941 cgctagtggt atttgtgtca acaccgctag agatatttta tcaccgcaaa tggttatctg
    45001 tatgtttttt atatggattt attttttcga gtgagtgtat tgcctcggcg atgtaaggag
    45061 agatcagaat tgcgttgttt agtgagttgt atctatttat tttccaataa atacaattgg
    45121 ttatgtgttt ttaggtgggg cgatcgtgag gcaaagaaaa cccggcgctg aggccgggtt
    45181 gtattatgct gcaagtctct taatccaagt ctctcttttg gagaatggta gaacttggct
    45241 tgattcatgg aagagtaagg agagttgttg catttgatca ccaattgctt cgctgtctat
    45301 aacaacaaat ctgttgttat actcctcact tgaagcttta agatcaatta gtttcccaag
    45361 caatgagtaa gcactattcc agcttcctcc gtgtttcacg cttgatgtaa aaacgtattt
    45421 aggtatgtcg gtttttattg ttacagggac ggtaatttga tgtccactca agccatatac
    45481 gttttcacga agagaaagtg cctctctaag ctctgtgtga tatagataat caataaccat
    45541 actttcaaac ttttcagctt gaactggctg ataccagtct aacgacaaag ttgatgcgag
    45601 tataccagct ctaattatgt tcgatgtaat cgcaccgaca tccttttctg ttgcccaagc
    45661 aatgatttct cctcgagcat tgagttctgc gccttcttta agcagtaatt gtcgtatctc
    45721 atcaagtcgt tttttggtaa gcgagatccc tcttgcctcc atattcatta aggcatcgca
    45781 tctgtcacta actaaatacc taccattgac ttcacggata aaggcaccga catgctctcc
    45841 atcatcacag taagtgaatg gactgataat tctcaaggtc ttgcctattg gatggcattc
    45901 gaaacctagt tgtgagatca ctgttgagca catcatattc caaaccccat ttgccccgac
    45961 tcatcttcaa gcggtaaagg tattctccca gagtaagtta tgttcagatg tttacagaag
    46021 taattccaat accctaccag gtcatctggg tttatgtcat cttcgattgg aaacgctatt
    46081 ctatcactat agtatccagc ttcttcatag tatacatggt aatgagcacc gtaaatgata
    46141 tcttggtact tcggatgatc gaccttgtaa ctattcgtgt gtttgtcaaa gtgataggta
    46201 tctaccgcga aaaccctctt gttatgatag aaagcaataa tatttatttt agggtaagaa
    46261 atcgggtcat caggttctga atcctgatct ggtttccatt tcagttcaaa ctttagccct
    46321 tgtataggta tacaatcctc atccaagggg atgatatgag cttgtagcca catatcagag
    46381 cgacttggtt tctgtttcca tttaacgcca gaaaagttaa ctattttttt acaataaagt
    46441 actttatcaa cttctacttg gcttggctga taatcatcta tttttgccaa tgtttaccgc
    46501 cctgtaaatt gtagataaaa gtggtttcat cacccaaacg tctcttcagg ccattggctg
    46561 gcgataactt tccccacaac ggaacaactc tcattgcatg ggatcattgg gtattgtggg
    46621 tttagtggct gtagaaacac ctgaccgcta tccctgatca gtttcttgaa ggtaaactcg
    46681 tcaccaccaa gtctggctat gcagaaatca ccaggctcaa cagtctgctc agggtcaaca
    46741 agaattaaca tcccgtcagg aaagcttggc ttgtagcctg ttggtgcggt catggaatta
    46801 ccttcaacct caagccagaa tgcagagcca ctggcttttt tagttgtgct tacccatttc
    46861 tccgcatcac ttttggtaaa ggttctaagc tcaggcgaga acatcccggc ctgaacatga
    46921 gaaaaaacag ggtactcata ctcacttcta agtgacggct gcatactaac cgcttcatac
    46981 atctcgtaga tttctctggc gattgaaggg ctaaattctt caacgctaac gttgagaatt
    47041 tttgcaagca atgcggcgtt ataagcattt aatgcattga taccattaaa taaagcacca
    47101 acgcctgact gccccatccc catcttgtct gcgacagatt cctgagataa gccaagttca
    47161 tttttctttt tttcataaat agctttaagg cgacgtgcgt cctcaagctg ctcttgtgtt
    47221 aacggtttct tttttgcgct catacattaa atctatcacc gcaagggata aatatctaac
    47281 accgtgcgtg ttgactgttt tacctctagc ggtgataata attgcatgta ctaaggaggt
    47341 tctatggaac aacgcataac cctgaaagat tatgcaatgc gctttgggca aaccaagacg
    47401 gctaaagatc tcggcgtata tcaaagcgcg attaacaagg ccattcatgc aggccgaaag
    47461 atttttttaa ctataaacgc agatggaagc gtttatgcgg aagaggtaaa gcccttcccg
    47521 agtaacaaaa aaacaacagc ataaataaca ccgctcttac acatcacagc cctgaaaaag
    47581 ggcatccaat taaaccacac ctatggtgta tgcatttatt tgcatacatt caatcaattg
    47641 ttatctaagg aaatacttac atatggttcg tgcaaacaaa cgcaacgagg cactacgaat
    47701 cgagagtgcg ttgcttaaca aaatcgcaat gcttggaact gagaagacag cggaagctgt
    47761 gggagttgat aagtcgcaga tcagcaggtg gaagagagac tggattccca agttctcaat
    47821 gctgcttgct gttcttgaat ggggggtcgt tgacgacgac atggctcgat tggcgcgaca
    47881 agttgctgcg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
    47941 ccagatggag ttctgaggtc attactggat ctatcaacag gagtcattat gacaaataca
    48001 gcaaaaatac tcaacttcgg cagaggtaac tttgccggac aggagcgtaa tgtggcagat
    48061 ctcgatgatg gttacgccag actatcaaat atgctgcttg aggcttattc gggcgcagat
    48121 ctgaccaagc gacagtttaa agtgctgctt gccattctgc gtaaaaccta tgggtggaat
    48181 aaaccaatgg acagaatcac cgattctcaa cttagcgaga ttacaaagtt acctgtcaaa
    48241 cggtgcaatg aagccaagtt agaactcgtc agaatgaata ttatcaagca gcaaggcggc
    48301 atgtttggac caaataaaaa catctcagaa tggtgtatcc ctcaaaacga gggaaaatcc
    48361 cctaaaacga gggataaaac atccctcaaa ttgggggatt gctatccctc aaaacagggg
    48421 gacacaaaag acactattac aaaagaaaaa agaaaagatt attcgtccga gaattctggc
    48481 gaatcctctg accagccaga aaacgacctt tctgtggtga aaccggatgc tgcaattcag
    48541 agcggcagca agtgggggac agcagaagac ctgaccgccg cagagtggat gtttgacatg
    48601 gtgaagacca tcgcgccatc agccagaaaa ccgaattttg cagggtgggc taacgatatc
    48661 cgcctgatgc gtgaacgtga cggacgtaac caccgcgaca tgtgcgtgct gttccgctgg
    48721 gcatgccagg acaacttctg gtccggtaac gtgcttagcc cggccaaact ccgcgacaag
    48781 tggacccagc tcgaaatcaa ccgtaacaag caacaggcag tcgtgacagc cagcaaacca
    48841 aaactcgacc tgacaaacac agactggatt tacggggtgg atctatgaaa aacatcgccg
    48901 cacagatggt taactttgac cgtgagcaga tgcgccggat cgccaacaac atgccggaac
    48961 agtacgacga aaagccgcag gtacagcagg tagcgcagat catcaacggt gtgttcagcc
    49021 agttactggc aactttcccg gcgagcctgg ctaaccgtga ccagaacgaa gtgaacgaaa
    49081 tccgtcgcca gtgggttctg gcttttcggg aaaacgggat caccacgatg gaacaggtta
    49141 acgcaggaat gcgcgtagcc cgtcggcaga atcgaccatt tctgccatca cccgggcagt
    49201 ttgttgcatg gtgccgggaa gaagcatccg ttatcgccgg actgccaaac gtcagcgagc
    49261 tggttgatat ggtttacgag tattgccgga agcgaggcct gtatccggat gcggagtctt
    49321 atccgtggaa atcaaacgcg cactactggc tggttaccaa cctgtatcag aacatgcggg
    49381 ccaatgcgct tactgatgcg gaattacggc gcaaggctgc cgatgaactg acctgtatga
    49441 cagcgcgaat taaccgtggt gaggcgatcc ctgaaccagt aaaacaactt cctgtcatgg
    49501 gcggtagacc tctaaatcgt gcacaggctc tggcgaagat cgcagaactc aaagctaagt
    49561 tcggactgaa aggagcaagt gtatgacggg caaagaggca attattcatt acctggggac
    49621 gcataatagc ttctgtgcgc cggacgttgc cgcgctaaca ggcgcaacag taaccagcat
    49681 aaatcaggcc gcggctaaaa tggcacgggc aggtcttctg gttatcgaag gtaaggtctg
    49741 gcgaacggtg tattaccggt ttgctaccaa ggaagaacgg gaaggaaaga tgagcacgaa
    49801 cctgattttt aaggagtgtc gccagagtgc cgcgatgaaa cgggtattgg cggtatatgg
    49861 agttaaaaga tgaccatcta catcactgag ctaataacag gcctgctggt aatcgcaggc
    49921 ctttttattt gggggagagg gaagtcatga aaaaactaac ctttgaaatt cgatctccag
    49981 cacatcagca gaacgccatt caagctatcc agcaaatcct tccagaccca accaaaccaa
    50041 tcgtagtaac cattcaggaa cgcaaccgca gcttagacca gaatcgaaag ctttgggctt
    50101 gccttggtga cgtctctcgt caggttgaat ggcatggtcg ctggctggat gcagaaagct
    50161 ggaagtgcgt ttttacagca gcattaaagc agcaggacgt tgttcctaac cttgccggga
    50221 atggctttgt ggtaataggc cagtcaacca gcaggatgcg tgtaagcgaa tttgcggagc
    50281 tattagagct tatacaggca ttcggtacag agcgtggcgt taagtggtca gacgaagcgc
    50341 gactagctct cgaatggaaa gcgcgatggg gagatcgggc tgcatgacta tcaaatcaaa
    50401 tacgccagca cacgacaagg actgctggca
//
LOCUS       DBANUU010000015        50255 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000015 DBANUU010000000
VERSION     DBANUU010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.015"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(104..391)
                     /gene="yebO"
                     /locus_tag="ACIE68_000863"
     CDS             complement(104..391)
                     /gene="yebO"
                     /locus_tag="ACIE68_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFX5907216.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(466..609)
                     /gene="mgrB"
                     /locus_tag="ACIE68_000864"
     CDS             complement(466..609)
                     /gene="mgrB"
                     /locus_tag="ACIE68_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFX5907217.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            768..1007
                     /gene="yobH"
                     /locus_tag="ACIE68_000865"
     CDS             768..1007
                     /gene="yobH"
                     /locus_tag="ACIE68_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFX5907218.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1150..1941)
                     /gene="kdgR"
                     /locus_tag="ACIE68_000866"
     CDS             complement(1150..1941)
                     /gene="kdgR"
                     /locus_tag="ACIE68_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFX5907219.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2118..3491
                     /gene="yebQ"
                     /locus_tag="ACIE68_000867"
     CDS             2118..3491
                     /gene="yebQ"
                     /locus_tag="ACIE68_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5907220.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAAFVSGLRI
                     TQPRSRA"
     gene            complement(3537..4418)
                     /gene="htpX"
                     /locus_tag="ACIE68_000868"
     CDS             complement(3537..4418)
                     /gene="htpX"
                     /locus_tag="ACIE68_000868"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFX5907221.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4610..6658)
                     /gene="prc"
                     /locus_tag="ACIE68_000869"
     CDS             complement(4610..6658)
                     /gene="prc"
                     /locus_tag="ACIE68_000869"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFX5907222.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6678..7376)
                     /gene="proQ"
                     /locus_tag="ACIE68_000870"
     CDS             complement(6678..7376)
                     /gene="proQ"
                     /locus_tag="ACIE68_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFX5907223.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7473..7970)
                     /gene="msrC"
                     /locus_tag="ACIE68_000871"
     CDS             complement(7473..7970)
                     /gene="msrC"
                     /locus_tag="ACIE68_000871"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFX5907224.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8100..9383
                     /gene="yebS"
                     /locus_tag="ACIE68_000872"
     CDS             8100..9383
                     /gene="yebS"
                     /locus_tag="ACIE68_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFX5907225.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9352..11985
                     /gene="yebT"
                     /locus_tag="ACIE68_000873"
     CDS             9352..11985
                     /gene="yebT"
                     /locus_tag="ACIE68_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFX5907226.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSVSGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12065..13504
                     /gene="rsmF"
                     /locus_tag="ACIE68_000874"
     CDS             12065..13504
                     /gene="rsmF"
                     /locus_tag="ACIE68_000874"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFX5907227.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQATAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13622..13858
                     /gene="yebV"
                     /locus_tag="ACIE68_000875"
     CDS             13622..13858
                     /gene="yebV"
                     /locus_tag="ACIE68_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFX5907228.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13963..14154
                     /gene="yebW"
                     /locus_tag="ACIE68_000876"
     CDS             13963..14154
                     /gene="yebW"
                     /locus_tag="ACIE68_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFX5907229.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14155..14811)
                     /gene="pphA"
                     /locus_tag="ACIE68_000877"
     CDS             complement(14155..14811)
                     /gene="pphA"
                     /locus_tag="ACIE68_000877"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFX5907230.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15207..15548)
                     /gene="yebY"
                     /locus_tag="ACIE68_000878"
     CDS             complement(15207..15548)
                     /gene="yebY"
                     /locus_tag="ACIE68_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFX5907231.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15561..16433)
                     /gene="copD"
                     /locus_tag="ACIE68_000879"
     CDS             complement(15561..16433)
                     /gene="copD"
                     /locus_tag="ACIE68_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFX5907232.1"
                     /translation="MLAFTWIALRFIHFTSLMLVYGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16437..16811)
                     /gene="yobA"
                     /locus_tag="ACIE68_000880"
     CDS             complement(16437..16811)
                     /gene="yobA"
                     /locus_tag="ACIE68_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFX5907233.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16950..17180
                     /gene="holE"
                     /locus_tag="ACIE68_000881"
     CDS             16950..17180
                     /gene="holE"
                     /locus_tag="ACIE68_000881"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFX5907234.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17282..17938
                     /gene="yobB"
                     /locus_tag="ACIE68_000882"
     CDS             17282..17938
                     /gene="yobB"
                     /locus_tag="ACIE68_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFX5907235.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17962..18624
                     /gene="exoX"
                     /locus_tag="ACIE68_000883"
     CDS             17962..18624
                     /gene="exoX"
                     /locus_tag="ACIE68_000883"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFX5907236.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18621..20681)
                     /gene="ptrB"
                     /locus_tag="ACIE68_000884"
     CDS             complement(18621..20681)
                     /gene="ptrB"
                     /locus_tag="ACIE68_000884"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFX5907237.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHYRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20890..21549)
                     /gene="yebE"
                     /locus_tag="ACIE68_000885"
     CDS             complement(20890..21549)
                     /gene="yebE"
                     /locus_tag="ACIE68_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFX5907238.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(21876..22232)
                     /gene="yebF"
                     /locus_tag="ACIE68_000886"
     CDS             complement(21876..22232)
                     /gene="yebF"
                     /locus_tag="ACIE68_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFX5907239.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22299..22589)
                     /gene="yebG"
                     /locus_tag="ACIE68_000887"
     CDS             complement(22299..22589)
                     /gene="yebG"
                     /locus_tag="ACIE68_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFX5907240.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22723..23901
                     /gene="purT"
                     /locus_tag="ACIE68_000888"
     CDS             22723..23901
                     /gene="purT"
                     /locus_tag="ACIE68_000888"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFX5907241.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIKRAKHAAGQVKVQG
                     "
     gene            complement(23957..24598)
                     /gene="kdgA"
                     /locus_tag="ACIE68_000889"
                     /gene_synonym="eda"
     CDS             complement(23957..24598)
                     /gene="kdgA"
                     /locus_tag="ACIE68_000889"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFX5907242.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24635..26446)
                     /gene="edd"
                     /locus_tag="ACIE68_000890"
     CDS             complement(24635..26446)
                     /gene="edd"
                     /locus_tag="ACIE68_000890"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFX5907243.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26681..28156)
                     /gene="zwf"
                     /locus_tag="ACIE68_000891"
     CDS             complement(26681..28156)
                     /gene="zwf"
                     /locus_tag="ACIE68_000891"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFX5907244.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28494..29363
                     /gene="hexR"
                     /locus_tag="ACIE68_000892"
                     /gene_synonym="yebK"
     CDS             28494..29363
                     /gene="hexR"
                     /locus_tag="ACIE68_000892"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFX5907245.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29491..30933
                     /gene="pyk"
                     /locus_tag="ACIE68_000893"
     CDS             29491..30933
                     /gene="pyk"
                     /locus_tag="ACIE68_000893"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFX5907246.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31064..32035)
                     /gene="lpxM"
                     /locus_tag="ACIE68_000894"
                     /gene_synonym="msbB"
     CDS             complement(31064..32035)
                     /gene="lpxM"
                     /locus_tag="ACIE68_000894"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFX5907247.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32155..33477)
                     /gene="mepM"
                     /locus_tag="ACIE68_000895"
     CDS             complement(32155..33477)
                     /gene="mepM"
                     /locus_tag="ACIE68_000895"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFX5907248.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33493..34425)
                     /gene="znuA"
                     /locus_tag="ACIE68_000896"
     CDS             complement(33493..34425)
                     /gene="znuA"
                     /locus_tag="ACIE68_000896"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFX5907249.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            34504..35259
                     /gene="znuC"
                     /locus_tag="ACIE68_000897"
     CDS             34504..35259
                     /gene="znuC"
                     /locus_tag="ACIE68_000897"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFX5907250.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35256..36041
                     /gene="znuB"
                     /locus_tag="ACIE68_000898"
     CDS             35256..36041
                     /gene="znuB"
                     /locus_tag="ACIE68_000898"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFX5907251.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(36382..37392)
                     /gene="ruvB"
                     /locus_tag="ACIE68_000899"
     CDS             complement(36382..37392)
                     /gene="ruvB"
                     /locus_tag="ACIE68_000899"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFX5907252.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37401..38012)
                     /gene="ruvA"
                     /locus_tag="ACIE68_000900"
     CDS             complement(37401..38012)
                     /gene="ruvA"
                     /locus_tag="ACIE68_000900"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFX5907253.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATNDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(38151..38216)
                     /gene="yobI"
                     /locus_tag="ACIE68_000901"
     CDS             complement(38151..38216)
                     /gene="yobI"
                     /locus_tag="ACIE68_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFX5907254.1"
                     /translation="MYIFITHFFTEYVILKYLLPK"
     gene            38287..38889
                     /gene="yebB"
                     /locus_tag="ACIE68_000902"
     CDS             38287..38889
                     /gene="yebB"
                     /locus_tag="ACIE68_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFX5907255.1"
                     /translation="MNINYPAEYEIGDIAFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(38891..39412)
                     /gene="ruvC"
                     /locus_tag="ACIE68_000903"
     CDS             complement(38891..39412)
                     /gene="ruvC"
                     /locus_tag="ACIE68_000903"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFX5907256.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(39447..40187)
                     /gene="yebC"
                     /locus_tag="ACIE68_000904"
     CDS             complement(39447..40187)
                     /gene="yebC"
                     /locus_tag="ACIE68_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFX5907257.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(40216..40668)
                     /gene="nudB"
                     /locus_tag="ACIE68_000905"
     CDS             complement(40216..40668)
                     /gene="nudB"
                     /locus_tag="ACIE68_000905"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFX5907258.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTCNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(40786..42558)
                     /gene="aspS"
                     /locus_tag="ACIE68_000906"
     CDS             complement(40786..42558)
                     /gene="aspS"
                     /locus_tag="ACIE68_000906"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFX5907259.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEDILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            42868..43434
                     /gene="yecD"
                     /locus_tag="ACIE68_000907"
     CDS             42868..43434
                     /gene="yecD"
                     /locus_tag="ACIE68_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFX5907260.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            43431..44249
                     /gene="yecE"
                     /locus_tag="ACIE68_000908"
     CDS             43431..44249
                     /gene="yecE"
                     /locus_tag="ACIE68_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFX5907261.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            44302..44697
                     /gene="yecN"
                     /locus_tag="ACIE68_000909"
     CDS             44302..44697
                     /gene="yecN"
                     /locus_tag="ACIE68_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFX5907262.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            44738..45481
                     /gene="cmoA"
                     /locus_tag="ACIE68_000910"
     CDS             44738..45481
                     /gene="cmoA"
                     /locus_tag="ACIE68_000910"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFX5907263.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HNAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            45478..46449
                     /gene="cmoB"
                     /locus_tag="ACIE68_000911"
     CDS             45478..46449
                     /gene="cmoB"
                     /locus_tag="ACIE68_000911"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFX5907264.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(46614..49043)
                     /gene="torZ"
                     /locus_tag="ACIE68_000912"
     CDS             complement(46614..49043)
                     /gene="torZ"
                     /locus_tag="ACIE68_000912"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFX5907265.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFDDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGHASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAIADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(49068..50168)
                     /gene="torY"
                     /locus_tag="ACIE68_000913"
     CDS             complement(49068..50168)
                     /gene="torY"
                     /locus_tag="ACIE68_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFX5907266.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKDSET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSHIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT        11908 a        13046 c        12360 g        12941 t
ORIGIN      
        1 agaccagagt tttaatttga agcagttgca ccatggcagt gcgtataatc aggatgaatt
       61 gagtaaccct tcaacgacgg ggttactcaa gattgaggaa ggattatcgt tcggcgacca
      121 gacgaataat atcttcgtct tcaaccgatt tttgactctc aacggtcttt ttatctgctt
      181 tctcaggttc gtttgcttcg cacaaacgac gtaacagtgc attttgacgt ttttgctgat
      241 ccagcaacgc ctcaagcagt tcaatctgtt cgttagtccg agaactggca cgattgataa
      301 aaaaccacaa gatgagcccg ataagaagaa ccaccaccga tacaaccaaa gacgcaatgt
      361 tcatcacgcc tgaatttaca acttcgttca tttcaccacc tctgagtaga ggcgctattc
      421 taccactgct ggagaggaag aaaatctagt gctgaaaaaa tgatatcacc acgggataaa
      481 ctggttaatg gcacaaattc cgctgaaaaa ttgtacatcc tgatcgcaca tcatgttgaa
      541 tacctgcgcc caaagcagca agcaagccaa caccacgaca accagaacga cccatcgaaa
      601 ctttttcact ccaatctccg cttcacctac cttatgtcat taaactagca tgcgttagtt
      661 aaacagcgcc taactatgtc gatattcgtg ctttttcgga atgagtagct tgcttcagcg
      721 catattgccg ctacgattaa gcgaacataa aaagagaaga ggttgtaatg cgattcatca
      781 ttcgcacagt tatgctgata gcactggtat ggataggatt attactcagc ggatatggcg
      841 ttttgattgg aagcaaagag aatgccgcag gattaggttt gcaatgtacc tatctgactg
      901 ccagaggcac cagcacggta caatacttgc acactaaaag tggtttcttg gggataacag
      961 attgcccact gttacgtaaa agcaatatcg tggtcgataa tggttaaggg taataaataa
     1021 ccagattctc actcatgagt aaaatattct tatagcaagc gcgtttaata taacgcacct
     1081 tcaactttat ttttctcatg ctaaataaag aacctgtctg tttgatattc cgacaggttc
     1141 ttactctcat cagaacggat agtcgtgata acccatttgg gcagaaattt tgcgcgctgc
     1201 ggtatgcaac attgcgacat attcctgtaa acgctcttca gagaaacgca acgtcgggaa
     1261 ggagatgctc aaaccggcaa tgaccacgcc aaagcgatca aataccggta ccgcaatgca
     1321 tcgcagccct tcttcctgct cttcgttatc ttcgccatac ccctgctcgc gcacctggtc
     1381 cagaacgggt aataacgctt ctgtactggt gatggtccgc tcggtactgc gtttatactc
     1441 cacgccctca agaatttgct tcacttcatc gcgatcgcgc catgccagca gtaccttacc
     1501 aatcgcggtg ctgtacagcg gattacgacg cccaatccgt gaatacatgc gcaaattgta
     1561 catagagtca attttgtgaa tgtaaacaat actgtcttcg tccagtgcgc cgaggtggat
     1621 agtttctttg gtcaggcggg agatctcacg catctggata tctgcgctac gaattaaatc
     1681 gacgttttgt aacgcgcgag cgcccagttc aaacaatttc aaggtcaggg aatatttctc
     1741 cgactcccct tcctgcgcca cataacctaa ggttttcatg gtctgtaaaa agcgataaac
     1801 ggtgcttttt gacatcatga cgcgctgcga cagctcggtt atccctattt cgcgctcttc
     1861 acccagcgcc tgcaaaatgc caaaaacttt tagcacggaa gatacagaat caggctgttt
     1921 atccagatct gcgttagcca tttatcacct cattgcgagt gttttataaa aatcagaact
     1981 gttttttatt ataatttcgc accagggtgg tcgcaatcca tcttttgccg gttagttaca
     2041 attctgcgac atccaccgtg aataacagtg ctagaatcat acccctgttg attattcacc
     2101 aaagatataa aattcctatg ccaaaagttc aggccgacgg cctgccattg ccccagcgat
     2161 acggtgcgat attaaccatt gtgattggta tttcgatggc tgtccttgac ggcgcaatcg
     2221 ccaacgtcgc cctgccaaca atcgccacgg accttcatgc cacgccagcc agttccatct
     2281 gggtagtgaa cgcctatcaa atcgccattg tcatctccct gctctcgttt tcgtttctgg
     2341 gcgatatgtt tggctatcga cgtatttata aatgcggtct ggtcgttttt ctgttgtctt
     2401 cactgttctg cgccctttct gattcgctgc aaatgctcac ccttgcgcgt gtcatacaag
     2461 gtttcggcgg tgcagcgttg atgagcgtta ataccgcact tatccgcctg atctatccac
     2521 aacgttttct ggggagaggg atgggcataa actcgtttat tgttgccgtc tcttctgctg
     2581 ccgggccgac aattgctgca gcaatcctct ccatcgcatc ctggaaatgg ttatttttaa
     2641 tcaacgtacc gttaggtatt atcgccctgc ttctggcgat gcgttttctg ccacccaatg
     2701 gttctcgcgc cagtaaaccc cgtttcgacc tgcccagcgc cgtgatgaac gcgttaacct
     2761 tcggcctgct tatcactgcg ttgagtggtt tcgctcaggg gcaatcgctg acgttaattg
     2821 ctgcggaact ggtggtaatg gttgttgttg gtattttctt tattcgccgc cagctttctc
     2881 ttcccgtacc gctgctaccg gtggatttac tgcgtatccc gctgttttca ctttctattt
     2941 gcacatctgt ttgctctttc tgcgcacaaa tgctggcaat ggtttccctg cccttttacc
     3001 tgcaaaccgt gctcgggcgt agtgaagtcg aaacaggttt acttctgaca ccgtggccgt
     3061 tagcaacgat ggtgatggct ccgctggcag gctatttgat tgaacgcgta catgcaggat
     3121 tgctgggtgc tttaggatta ttcatcatgg ctgcggggct tttttccctg gttctgctgc
     3181 ccgcgtcacc tgcggatatc aatattatct ggccgatgat cttatgtggc gctggatttg
     3241 gcttgttcca gtcacccaat aaccacacca ttattacctc cgcgcctcgc gaacgtagcg
     3301 gtggagccag tggcatgtta ggaacggctc gtctactggg tcagagtagc ggcgcggcgc
     3361 tggtggcgct gatgctaaat cagtttggag ataatggtac gcacgtctcg ctgatggctg
     3421 cggctattct ggcagtgatt gctgcctttg tcagtggttt acgtatcact cagccacgat
     3481 ccagggcata aaaaaagcgc gtcgatcagg acgcgctttt tagtatttac ttcatattac
     3541 ttcaggtatt cacccgtacg cagagcttca atacgtttat ccagcggcgg gtgagtcatg
     3601 aacaactcac tgagcgattt cgacttaccg ttaatgcaga aagccatcat gctggttgct
     3661 tcttgcggtt catagctggt tttcaggcgc tgcagcgcgg caatcatttt ctcgcgacca
     3721 accagttttg ccgaaccggc atcagcatgg aattcacgat gacgcgagaa ccacatggtg
     3781 ataatgctcg ccagaatacc aaacaccagt tccagaaccg ttgcaaccgc aaagtagatc
     3841 agcgggttgc cgttgctctc ttcaccttca tcacgatttc cgcccataaa acccgcggca
     3901 agctgcgcca ggatacggga aataaagata acgaaggtgt tcaccacgcc ctgaataagc
     3961 gtcatggtga ccatatcacc attggcgatg tggctgattt cgtgagcaat taccgcctcg
     4021 gcttcatccg ggctcatgtt ctgcagcaaa ccggtgctga cagcaaccag agaggcatca
     4081 cggcgcgcac cggttgcaaa agcgttgatg tccggcgcat ggtagatagc cacttgcggc
     4141 atagcgatcc ccgcctgacg agcctgggtt gctacagtat tgaccagcca acgttccctt
     4201 tcgttacgcg gttgctcgat cacttccccg ccaacagatc gtaatgccat ccatttggac
     4261 atcagaagcg aaacgaagga accaccaaaa ccgaacagca aggccatgat catcagcccc
     4321 tgaacgctgc tcgactgtat ccctgtcagg ctcagtacca gcccgaaaac gaccattacg
     4381 gccaggttcg ttagcaggaa gagcgcgatt cgcatcataa ttttcttttt acctcagttt
     4441 aacaaaacgc aatatgcgat acccacatcg tatgggttac gcgactattt tcaagtctgg
     4501 atagtgcgta agtcaccaga aagacacaac tttacatttt gtagcatctg atttacggca
     4561 tcttgccgct gttaaaaaat caggcacaat ttcttgtgcc tgattgatat tacttgacgg
     4621 gagcgggttg ttccgcgggt ctggcttttt caagcttcgc cagatcgagt gcgatattca
     4681 ccgtctcatc cagataagga tccggctcct ggtaatcttt tggtagatca tccagcttct
     4741 tcaactccgg tttaccttcg cgtttaaagc gttcgttcaa acgcgccaga cgcgtcgcat
     4801 catcttcatt attctctttc tcacgcacag cgtaattcag agaaacgata ttgcgcttgt
     4861 ccttcatagc gttgaagcgc gcgatatcct tcatgatgtt ctggaactca ggatctttcg
     4921 cgatacgcgc attatgttcc ttcagtagct ccggttcaaa ggccgttaaa tctcctgatt
     4981 tcacataagt cgcggcatca atgctatccc acggcagcgc gttatcttcg aatttctcac
     5041 ccgtttccgt ttcttcatta cccgtcggca tgatgatgtc tggcgttacg cctttacgtt
     5101 gcgtactgcc gccgttaacg cgatagaatt tctggatcgt gtactgcaca gaacccagcg
     5161 ctggccattc aggacgtaac atctgatcgt aaatacggtt caatgaacgg tattgctgaa
     5221 cggtgccttt accaaacgtc ggttcaccaa caaccagcgc acgaccgtag tcctgcattg
     5281 ccgcggcaaa gatttctgaa gccgaagcac tgaagcggtc aaccagtacc accagcgggc
     5341 ctttatagaa gacctgtccg tcggtatcgc tgtcttcacg aaccttgccg ttgttatcac
     5401 ggacctgaac aatcgggccg gatggaatga acagacctga gagcgataca gcttcggtca
     5461 acgccccacc gccattgcta cgcaggtcga tgatcacact gctgacattc tgtttttcca
     5521 gtttctgcag ttgtactttg acatcgtctg tcaaacccac atagaagccc ggaatatcca
     5581 gcacgccgac tttctcttta ccgacggtct tcaccgacat tttaaccgcg cggtcttcga
     5641 gacgaatacg ttcacgggtc aatgttacag tgcgggtctt ggtcccttta ccagcaggta
     5701 aaatttccag acgaacttta ctgcccttcg gccctttaat taaggcaacc acatcatcaa
     5761 gacgccagcc aatcacgtca accatcggct tgcctgtttg accaacaccg acaattttgt
     5821 caccaacgct gatagcttta ctcttcgctg ccggaccacc tgccaccatc gaattgataa
     5881 cggtgtagtc atcatccatt tgcagcactg cgccaatacc ttccagcgac aaactcattt
     5941 cagtgttgaa ctgttcggta ttacgcgggg aaagatagtt ggtatgcggg tcgatttcac
     6001 gcgcaaacgc cgtcattgcc agcgagaaaa catcttcgct gttggtttgc gccagacgac
     6061 gaatggcaaa tttgtagcgg cgtgtcaggg tttcacgaat ttctttatcc gtttttcctg
     6121 ccagcttcag gcttaactcg tcgaatttga ctttactgtc ccacagcgcg ttcaactcag
     6181 cctcgttttt cggccagggc gctttgctgc ggtcaaggtt ataagtgtcg ttgccggtga
     6241 aatccatcgg cttttccagt accgacaaag cgtactggta acgctcaaaa cggcgctttt
     6301 gcgccagatt gtagagatcg tagaaaacgt cgagtttgcc tgaacgcagt tcatcgccta
     6361 actcggtttt ctttttcgcg aactgttcaa catcgcttgc cagcagcacg ttgtggctgt
     6421 aatcgagcag attcaggtag cggtcaaaga ttttggccga aaatgcctga tcgaggtcga
     6481 actggcgata atgagaacgg gtgaagcgcg acgttacgcg ctcacttacc gtcgcatgct
     6541 gcgtctcttc ctttaatacc ggaatttgat cagcacgcgt gatatcttct acagcgaagg
     6601 tctggcctgc tattgcaagc aggccagcta acgcggtaag cctaaaaaac atgttcatgc
     6661 ctggcccggc ctccgtttca gaacactagg tgttctgcgc gcacaatcaa agacataccc
     6721 gaattcagct ggacgcggac gccgtctttg gtgatttcta atacggtggc atccatcgcg
     6781 ttttgacccg ctttcacctt cagggcttgt ccgacagtca gagctgaaat gtcagaaact
     6841 ggggtgtgct gttcttcgcg aggtgctttt actgtttttg gcgctttctc taccggcttt
     6901 tgcgcacgag gtttacgttc agcgccttct ttgcggcgtg gcgtggtcgg acgtggcttg
     6961 cgttcgcggc gcggtgcgtc ttctttctca ccagcagctg cggcagcttc gcgttttttc
     7021 gcttgctgtt cagcacgctg tgcctgaaca cgcgctttcg cttcttcaag ctgcttgcga
     7081 gcatgctcta catgttgctc gtccagctca ccgcatgggt tgccgtcaag atcgacacgc
     7141 gttgcgccgg gtttaacacc gtaaagataa cgccagctcg aagtgtagag acgtaaagcg
     7201 gatcgtaatt gcgttttgct caggttcatt tccccggcaa cacgatcgac caaatcctga
     7261 aaaataccga ttttcagcgg acgcgcttca ccttccgcac tgaaacagtg gggaaaacgt
     7321 tcggccagaa acgcgattac ttctttactg ctattcaact taggttgatt ttccatgaaa
     7381 tttcctgatt acaacggacg tagccaacaa gcgcaggcat gaacaggcgt cattataatg
     7441 acgctaccag taaatgctac gttatccgtt gattatcctg cgacgctcgc aaagaatttt
     7501 ttgtaatccg tcgttgcaag cactttttca agctgtgcca caagctgacg taagccttgc
     7561 tcgtcctcgt ctgtaaagcg accgaagacg gtactatcga tgtcaagaac accaataatc
     7621 tgatttttca ccaccagcgg cagaacaatt tcagaattac tcgccgcatc acaggcaata
     7681 tgcccgtcaa acgcgtgaac atcctcgata cgctgcactt gattgcgggc aaccgcagtg
     7741 ccgcacaccc cgcgcccgac gggtatccgg acacaggcaa ttttgccctg aaatggtcct
     7801 agtaccagtg tatcgtcctc aagcaaataa aaacctgccc agtttacgtc agtgagacgc
     7861 tcgtataaca acgcactggt gttcgcaagc gttgccagaa aactggtttc tcccgccatc
     7921 agcgcgttaa agtcgcgatt taaatccgcg taaaattctg ttttgttcat tatataatca
     7981 cttggttgtc ttacctggaa ctgccagcct attaaaataa gcattaaatg cgttaatgct
     8041 caagatcatt ccaatcatgg gttaatatta atgttaattc ttattacatt tggcacgtca
     8101 tggctcttaa cacaccacaa attacgccga caaaaaagat aacagtgagg gcaatcggcg
     8161 aggaactgcc gcgtggtgat taccaacgtt gcccgcaatg tgacatgctg tttagcctgc
     8221 ccgagataaa ttctcatcag agtgcctatt gtccgcgctg tcaggcaaaa attcgcgacg
     8281 ggcgcgactg gtcgctaacg cgcctggcgg caatggcttt caccatgctg ttgctgatgc
     8341 cgtttgcctg gggcgaaccg ctgttgcata tctggctgtt aggtattcgt atcgacgcca
     8401 acgtcatgca aggcatctgg caaatgacca aacagggcga tacgataacg ggggcgatgg
     8461 tctttttctg cgttattggt gcccccctca ttctggtgtc ctccatagca tatttatggt
     8521 ttggtaaccg actgggaatg aatttacgtc cggtactgct gatgcttgag cgtcttaaag
     8581 agtgggtaat gctggatatc tacctggtcg gcattggcgt tgcttctata aaggtacagg
     8641 attatgccca tatccaggct ggtgtcggct tgttctcttt tgtggcgttg gtgattttaa
     8701 cgacggtgac gttgtcacat cttaatgttg aggagctgtg ggagcgatat tatccgcagc
     8761 gccccgctac gcgtagggac gagaaacttc gtgtctgtct tgggtgccat tttaccggct
     8821 atcctgatca gcgtggtcgc tgcccgcgtt gccatatccc gctacgcctg cgtcgccgtc
     8881 atagtctgca aaaatgctgg gcggcgctgt tagcgtcaat cgttttgttg ttaccagcca
     8941 acctattgcc tatttctatc atttatctga acggaggacg gcaggaagat acaattcttt
     9001 ccggaattat gtcgctggca agtagcaaca ttgcggttgc gggaatcgtg tttatcgcca
     9061 gtattctggt accgtttact aaagtgatcg tcatgttcac tttactgttg agcattcatt
     9121 ttaaatgcca gcaaggttta cgcacacgca ttctgttact gcggatggtg acctggattg
     9181 gacgctggtc gatgctcgac ctgtttgtca tatctttaac catgtcgctg attaatcgcg
     9241 atcagatcct cgcttttact atgggaccgg ctgcgtttta ttttggcgca gccgtaattt
     9301 tgactattct tgctgtggaa tggctggaca gccgcttact ttgggatgca catgagtcag
     9361 gaaacgcccg cttcgacgac tgaagcgcag attaaaaata aacgccgtat ctcacctttc
     9421 tggctgctgc ctttcatcgc gctaatgatt gccggttggc tgatttggga cagttatcag
     9481 gaccggggta ataccgtcac catcgacttt atgtcggcgg atggtattgt tccgggccgt
     9541 acgcctgttc gttatcaggg cgttgaagtc ggaacagtgc aggatatcag cctcagcgac
     9601 gatcttcgta agattgaagt caaggtcagc atcaagtccg atatgaaaga tgcgctgcgc
     9661 gaagagactc aattctggct ggtgacgcca aaagcatcgt tggcaggtgt ctccgggctg
     9721 gacgccctcg tcggtgggaa ctatatcggc atgatgccgg gtaaaggtaa agagcaggat
     9781 cactttgtcg cactcgatac ccaaccgaag tatcggctgg ataacggcga tctgatgatc
     9841 cacctgcaag ctcccgatct cggttcgctg aacagcggtt cattggtcta tttccgcaag
     9901 atcccggtgg gaaaagtcta cgactatgcc atcaatccca acaagcaagg cgtggtgatt
     9961 gatgtcctga tcgagcggcg ttttaccgat ctggtgaaaa aaggtagccg tttctggaac
    10021 gtttccggcg ttgatgccaa cgtcagtgtc agtggcgcga aggtgaaact ggaaagtctg
    10081 gcggcactgg ttaacggtgc gattgccttc gattcacctg aagagtcgaa acctgccgag
    10141 gcggaagata cctttggtct gtatgaagat ctggcccaca gccagcgtgg cgtaataata
    10201 aaactggaac tgccgagtgg ggccggattg accgccgact cgacgccgtt aatgtatcag
    10261 gggctggaag tcggacagct aactaaactg gatttaaatc ctggtggtaa agtcactggg
    10321 gaaatgacag ttgatcccag cgtcgttacc ctgcttcgtg aaaatacccg catcgaatta
    10381 cgtaatccga aattatccct tagcgatgcc aatctcagcg ccctgctgac tggcaaaacc
    10441 ttcgagttgg tacccggcga tggcgagcca cgcaaagagt tcgttgttgt gccaggcgaa
    10501 aaagcactgc tgcatgaacc tgatgttctg acgctgaccc tgaccgcgcc ggaaagttac
    10561 ggtattgatg ctggtcagcc gctcattctt cacggcgtgc aggtaggcca ggttatcgat
    10621 cgtaaactca ccagcaaagg cgtcaccttt accgtcgcca tcgagcctca gcatcgagaa
    10681 ctggtaaaag gcgatagcaa atttgtcgtc aacagccgtg tcgacgtgaa ggtggggctg
    10741 gatggcgttg agtttctcgg tgccagcgcc tcagaatgga ttaacggcgg gatacgtatt
    10801 ctgccgggcg ataaaggcga gatgaaagcc agctatccgc tgtatgccaa tctggaaaaa
    10861 gcgctggaga acagccttag cgatttaccc accacaaccg tgagtttgag tgcagagacg
    10921 ctgccggatg tgcaggcagg atcggtagtg ctctaccgta aatttgaagt tggtgaagtt
    10981 attaccgtcc gtccgcgagc taacgcgttt gatatcgatc tgcatattaa gccggagtat
    11041 cgcaaccttc tgaccagcaa tagcgtgttc tgggcagaag gcggggcgaa agttcagctg
    11101 aatggtagtg gcctgaccgt acaggcatcc ccgctctcca gagcattaaa gggagccatt
    11161 agcttcgata acctcagcgg tgccagcgcc agtcagcgta aaggcgacaa acgaattctg
    11221 tatgcttccg aaacagcggc ccgtgcggtt ggtgggcaga ttacgcttca cgctttcgat
    11281 gccggaaaac tggcggtcgg gatgccaatt cgctatctcg gtattgatat cgggcaaatc
    11341 cagacgctgg atctgattac cgcgcgcaat gaagtacagg caaaggcggt gctctatccg
    11401 gagtatgtcc agaccttcgc ccgcggcggt acgcgcttct cggtggtcac accgcaaatt
    11461 tcagccgcgg gcgttgagca tctagatact atcctccagc cgtatatcaa cgtcgaacca
    11521 ggccggggca atcctcgccg cgactttgaa ttacaagagg ccaccattac tgattcgcgt
    11581 tacctggatg gcttaagcat tattgttgaa gcgccggaag ccggttcgtt aggtattggt
    11641 acgcctgtgc tgttccgtgg tctggaagtc ggtacggtta caggaatgac gctggggaca
    11701 ttgtcagatc gcgtgatgat tgcgatgcgc atcagtaaac gctatcaaca cctggtgcgt
    11761 aacaattccg tcttctggtt ggcatcgggt tacagtctgg actttggtct gacgggcggc
    11821 gtagtgaaaa ccggcacctt taaccagttt atccgtggcg gcatcgcctt cgccacgcct
    11881 ccgggtacgc cactggcacc gaaagcccag gaaggcaaac acttcctgtt gcaggaaagt
    11941 gaaccgaaag agtggcgtga atggggaact gcgcttccca aataatgccc actgctccgg
    12001 cgtgcctgcg ccggagcgtt tatgctaaac tgcgcgcctg tttttttgcc agtggtacat
    12061 gctcgtggcc caacacaccg tttatttccc ggacgccttt ctgacacaaa tgcgcgaagc
    12121 gatgccttcg acgctctcat ttgatgattt tcttgccgcc tgtcagcgcc cgttgcgccg
    12181 cagcattcgc gttaatacgc tgaaaatctc cgttgctgat ttcctgcaat taaccgctcc
    12241 ttatggctgg acgcttacgc caattccgtg gtgtgaagaa ggtttctgga ttgaacgcga
    12301 caatgaagat gcattgccat tgggtagtac cgccgagcat ttaagcggcc tgttttatat
    12361 tcaggaagca agttcaatgt tgcccgttgc cgccttgttt gctgacgata atgcaccaca
    12421 gcgggtgatg gatgtcgcag ctgcgccagg ctccaaaacg acgcaaattg ccgcgcggat
    12481 gaataacgaa ggggcaatcc ttgccaatga gttttccgcc agtcgggtaa aagtgttaca
    12541 tgccaatatc agccgctgtg gcatcagtaa tgttgcgctc acacattttg atggccgcgt
    12601 gtttggtgcg gcagtgccag aaatgttcga tgccattttg ctggacgctc cctgctctgg
    12661 cgaaggcgtg gtgcgtaaag atcccgatgc gctaaaaaac tggtcaccag aaagcaatca
    12721 ggaaatcgca gctacacaac gtgagcttat cgacagcgcc tttcatgcat tacgtcctgg
    12781 tggtacgctg gtttactcga cctgtacctt aaaccaggaa gaaaacgaag ccgtttgcct
    12841 gtggctgaaa gagacttacc ccgacgcagt agagttttta ccacttggcg atctcttccc
    12901 tggtgcaaac aaagcgctga ccgaagaagg ctttttgcat gttttcccac aaatttacga
    12961 ctgcgaaggc ttcttcgttg ctcgtctgcg taaaactcag gcgattcccg ccttacccgc
    13021 ccccaaatac aaagtcggta attttccgtt cagcccggtg aaagatcgcg aagctggaca
    13081 aattcgtcag gcgactgcag gtgttggctt aaactgggat gaaaacctgc gcctctggca
    13141 gcgtgacaaa gaactgtggt tgttcccggt gggcattgaa gccctgatcg gtaaagtccg
    13201 attttctcgg ttggggatta aacttgccga gacgcacaac aaaggttatc gctggcagca
    13261 tgaagcggtt attgctcttg cctcacccga taatatgaat gcattcgagc tgacaccgca
    13321 ggaagcggag gagtggtatc gcgggcgcga tgtttacccg caagccgcgc cagtggcgga
    13381 tgatgtattg gttactttcc agcatcaacc gattggttta gccaaacgga ttggttcgcg
    13441 attgaaaaac agctatccgc gtgaactggt gcgcgatggg aaacttttta ccggtaacgc
    13501 ctgacagctc acaaaaaaag cgcacttttt gactggtaca ttcggctgcc tcaactaggc
    13561 tgaaaaatgg tgcgatcgga ctggtcgtac cacaatcggc agctaaatgg agagcacgaa
    13621 gatgaaaacc agtgtgcgca taggcgcttt tgaaatcgac gacggcgaat tacacggtga
    13681 atcgccgggt gatcgaacgt taaccattcc ttgtaaatct gaccccgatt tatgtatgca
    13741 actcgatgcc tgggatgctg aaaccagtat ccctgccctg ctaaatggcg aacactctgt
    13801 cctttaccgt acccgttacg atcaacaatc tgatgcctgg attatgcgtc ttgcctgatc
    13861 caaaaagaac ccgtcggcat ggcgggttat ttgtcctggt tattcccccg ttgtaaaatc
    13921 tctcctaaac ttaacggtac ggcaccacac ttcggggatg aaatgttcgc gctggtactt
    13981 tttgtttgct acctggatgg cggttgtgaa gatattgttg tggatgtcta caacacggaa
    14041 cagcagtgtc tttattctat gagcgatcaa cggatccgcc atggcggttg ttttccgatt
    14101 gaggatttta tagatggttt ctggcgacca gcacaggagt atggtgattt ttaattattg
    14161 caattgcaca agagtcagtt cgcccccaaa gacagcaccg gtatcaatat aatgcaggtt
    14221 gccaatatcc acgcgatgtc gcaacggtgt atgaccaaac cagaaatgat cagcacctgt
    14281 aattccctgc cctttttggc gttcacctaa tcgcgagcgg ctccacaaga cctgatgcaa
    14341 atcaacgtcc ttttgccatt cataaacatc atctggataa tcggcatgag caataacatg
    14401 tttgccggta cgactgtgta cttcaagaat aaagggcaaa tgctgacatt tttccagcgc
    14461 cgttttcgct tgtttctgtt gattatctgc cagcgcaata aaccagtcgc cgccattcat
    14521 caaccacaaa gacatctgct gggatgccag cgcatccatc gccatctgtt catgattgcc
    14581 tcttaccgca caaacccaat gttgttccag taactgcaga caacgtaaac tttgcggccc
    14641 acgatcgata acgtctccta ctgagataag taaatctcgc cacggatcaa aacgacaatg
    14701 ccataatttg cggcgcaact gctcaagaca accgtgtata tcgccagaaa gccagatatg
    14761 tcgccattga tgacccgcaa ttctctgata aacgggcgca ggctgtttca tcaatatttt
    14821 cctcccgcgc taaagatcac ataatcttaa caagaatgtt aaaaaacgct ggactcagac
    14881 agtagagtgt gtgttatggt tgactataaa gtcagcgaag gaaatgcttc tggcttttaa
    14941 cagataaaaa gagaccgaac acgattcctg tattcggtcc agggaaatgg ctcttgggag
    15001 agagccgtgc gctaaaagtt ggcattaatg caggcttagt tgccttgccc tttaagaata
    15061 gatgacgacg ccaggttttc cagtttgcgt gcaaaatggt caataaaaag cgtggtggtc
    15121 atcagctgaa atgttaaaaa ccgcccgttc tggtgaaaga actgaggcgg tttttttatt
    15181 ggaaatcaaa aggctatttt aggtaattaa cagagttttt cagctcgttc tataaacggt
    15241 gccagactca ttttttcgcc gggattgtta ggatcatcaa tctgaatcac cgaaatgggt
    15301 tgggctttag tcttcccact ggcaacttcc ttttgtgcga tatcgtttaa aggatactgc
    15361 acgagggtac ttgggttaat gacatacaaa gcattacccg gtctgcaagt cagcatcacc
    15421 tcttcgcgat taaacgccca tttgtcttta cccacttcaa aacgactgac ggtaatcacc
    15481 tgcggtgcag ccagcgccgc tgcagaactg gtgagtaaca gaaacgtcag aatacttttt
    15541 ttcatcattt tcgtaatcca tcaaaaaggt tcgagagtcg caagcaggct gatgatcacc
    15601 agcactaccg cacctatcgc ccattcgagt ttagtcatcc actcaaacca gggtgccgca
    15661 cgatcttcat cttgcctcat gcgcggtaca agaacataac gattagccag cgcaatgacc
    15721 accataatca tcaccaggat ggctttgagc aataagagtt ggccccagta agtcgtaagc
    15781 gtggggggaa atccggtaat caacaatgca ttaagcacgc cgcttgccag tacgccgatc
    15841 accgcaaaat gcccgcacca ggaaaaacgc atcagcgcct gaatggcctg gtgtcgccaa
    15901 cggcctttga tgagttgcat acaccagagc accggcagta atcccccaaa ccacgccgcc
    15961 gcacaaatca ggtgaatcgc gtgattagtc tgatggattt tcgccgttac cccttcattc
    16021 agtgtcgcat gcccgattcc cgccagcaga ataaattgcg cggtagtgag cataaacagc
    16081 aaacgtggca tattccgcgg ttgcataagg gcgacgatca acgttacgag ggcgagaaca
    16141 atttgccata accagacgcc accaaactgc gtttgcaaaa cagcctgcca aatgtttggc
    16201 gaaaatacat ctgcccatcc cgttcccatc aacccgccct gaacggcgag cattgcagtg
    16261 gcactgatca aactccagac ggcagcgtgc tgttgtaaac gcagaaaacg cttcgccagc
    16321 aagcgacgaa tcgttaaggg ggccagccag gcaccgtaca ttgcgaagcc atagaccagc
    16381 atcagcgagg tgaaatggat aaatcgcagc gcgatccagg taaacgccag catgatttat
    16441 ttcacgctaa aggtgtagtg tcctttcgtt ttatgcccgt ccaccgaaac aacatgccag
    16501 tcaacgatgt aggttccggg tttcagcgaa tcggccagtg gaacaatcag ttgcttttgg
    16561 tcctgctcat ttcgcttcgc cggtaatgtt ttaatatttt cgttttttgg ccccgtgatt
    16621 tttgcaccac tgaatcctgt ttcaacacct tccgagaagt ttaaagtgat tgcctgcggt
    16681 gcagctgtca cttgcgcgtt tgccgcagga tactgatgcg ttaaatgcgc atgtgcccag
    16741 acggaaggtg tcaccaacga agtggtcagg attgccagcg cgtagcgaag ggagcgtgca
    16801 gttgaagtca tattatctat tcctttttgt aataactttt ttacagagca taaccttgtc
    16861 taatgtctga gtcgaggatc atcaattccg gcttgccatc ctggctcact cttagtaact
    16921 tttgcccgcg aatgatgagg agattaagaa tgctgaagaa tctggctaaa ctggatcaaa
    16981 cagaaatgga taaagtgaat gtcgatttgg cggcggccgg ggtggcattt aaagaacgct
    17041 acaatatgcc ggtgatcgct gaagcggttg aacgtgaaca gcctgaacat ttgcgcagct
    17101 ggtttcgcga gcggcttatt gcccaccgtt tggcttcggt caatctgtca cgtttacctt
    17161 acgagcccaa acttaaataa aacttataca gagttacact ttcttacata acgcctgcta
    17221 aattatgagt attttctaaa ccgcacccat aatttgcagt cattttgaaa aggaagtcat
    17281 tatgtcgttc tggaaagttg cagcagcgca atatgagcct cgtaaaacct cactcaccga
    17341 acaggtagct catcatctgg agtttgtgag ggccgccgcc aggcaacaat gtcagctgtt
    17401 agtttttcca tcgctttctt tactggggtg tgattattcg cgacgtgccc ttcctgcccc
    17461 acccgatctc tcactgttag acccgctttg ttatgccgca acgacctggc gaatgaccat
    17521 cattgccggc cttcccgttg aatataacga tcgctttatt cgtggaattg cggtgttcgc
    17581 accgtggcga aaaacgcctg ggatctacca tcagagtcat ggtgcatgtc tgggtcgtcg
    17641 ttccagaacc atcacggtgg tcgatgaaca accgcaaggt atggatatgg acccgacctg
    17701 ttcactgttt actacagggc aatgtctggg agaacccgac ctgctggcgt cggcccgtcg
    17761 cttacaattt ttttcacatc agtactcgat tgccgtgctg atggcaaatg cccgtggtaa
    17821 cagtgcacta tgggatgaat atggtcgtct catcgttcgc gccgatcgtg gttcattatt
    17881 gttagtcggt cagcgttcat cacagggttg gcaaggcgat atcattccat tacgctaggc
    17941 tttttcggcc tggagcatgc catgttgcgc attatcgata cagaaacctg cggtttgcag
    18001 ggagggatcg ttgagattgc ctctgttgat gtcattgacg gaaaaatcgt caaccccatg
    18061 agccacctgg tgcgccccga tcgtcctatt agtccacaag cgatggcgat tcatcgcatc
    18121 accgaagcca tggtcgccga taaaccgtgg attgaagatg tgatcccaca ctattacggt
    18181 agtgaatggt atgtcgcgca taacgccagc tttgaccgcc gcgtactgcc tgagatgccc
    18241 ggtgagtgga tttgcactat gaaactggcc cgtcgtttgt ggcctgggat caagtacagc
    18301 aatatggcgt tatataaaac acgcaagctc aatgtacaga cgccgccggg cctgcatcat
    18361 caccgcgcgt tgtatgactg ttatatcacc gccgcgttgc ttatcgatat tatgaacacc
    18421 tccggctgga cggcagaaca gatggccgat atcaccggac gtccgtcgtt gatgacgacc
    18481 ttcacctttg gcaaataccg tggcaaagcg gtttccgacg ttgccgaacg cgatccgggc
    18541 tatctgcgct ggttatttaa taacctggac agcatgagcc cggagctgcg tttaacactg
    18601 aaacattatc tggaaaatac ttagtccgca ggcgtagcgg gtaatgttcc ctgcgccagc
    18661 gcgaccagaa aagcatattc catcgctacg ccttcgtacg atttaaagcg accagattta
    18721 ccgccatggc ctgagtccat gtcggtacag agcaataaaa gatggtcatc ggttttcagc
    18781 tcgcgcaatt tagcgaccca ttttgccggt tcccaatatt gcacctgaga atcgtgcaaa
    18841 ccggtcgtta ccagtaaatg cggataagcc tgtgcggtga cgttgtcata cgggctgtag
    18901 cttttcatgt actcgtaata ttgcggatcc tgcgggttac cccactcttc aaactcacca
    18961 gtggtaagag gaattgattc atcaagcatc gttgttacaa catcaacaaa cggtacctgg
    19021 gcgataacgc cgtggaataa ttccgggcgt tgattaattg caacgcccat caacatgccc
    19081 cccgcactcc cgcccatcgc ataacaaagc gaaggagagc catagcccag ttttaacaat
    19141 gcatcgcagg catcaagata atcattaaac gtatttttct tcttcagaaa ttttccgtct
    19201 tcgtaccatt gttgccccag ctcaccaccg ccgcgaacat ggacaatggc gtagacaaag
    19261 ccacgatcta acaaactcaa gcggctaaaa ctgaaatcgg catcaatact tgcgccgtaa
    19321 gaaccatagc catacaccag caacgggttg tgtcctttgc gataatgttt gcgatggtag
    19381 accaacgaaa ccggaacttc gacgccatca cgggcgacta tccacaggtg ttcactgcgg
    19441 taattcgccg catcaaaacc gggcacttct gtttgtttta atacacgacg ctcaccggta
    19501 tccatatcca gttcaaacaa agtgtctggt gtggtcatgg aagaataacc ataacgcaat
    19561 cgcgcggttt caggttctgg attgtaggca atccaggtca cataggctgg atcatcaaag
    19621 gcaataccaa tgacttcccg ggtcttgcgg ttaatttggc gcaaactggt taacccgcgc
    19681 tgacgctctt caaccaccag ccagtcggta aacagcgtaa acccttccag catgatgttt
    19741 tcgcgtggcg gaattaactc ttcccactgt tgctcatcac gcatacgggt acggtataag
    19801 ccaaagtttt tgccgtggcg gttggaacgt agataaaaac gatgctggta gtgatcaagg
    19861 ctgtattcgt gatctttgcg gcgcggcaga aaaacaaacg gctcggcatc ggccatttcc
    19921 gcgtccagca ggcgaacttc actggtggtg gcgctggcca aatgaatgac tacatagtgc
    19981 ttcgaggtcg ttttatgcag gctgacgtaa taggtatcgt ctttttcttc gtagatcagt
    20041 ttatcttgcg atgctggcgt accgatggcg tgacgccaga cctgataagg cagcagcgtc
    20101 accggatgct tgcgaacata gtagaaagtc caggagtcat ttgcccagac aaagctgggt
    20161 tcaacgttat ccagcagttc cgggtaccag ttaccagttt ccagattacg aaaacgaatg
    20221 ccgtactggc gtcgggaaag aaaatcttct gccagcgcca taatggtgtt atcgggcgta
    20281 atcgccattc cgcccatcga ataaaactca ctatgagctg cgcgcttatt ggcatcgagc
    20341 aatgtttccc actcatccca ctcttcactg aatgccgatt gacgctggta gatagcatat
    20401 tcacagcctg gttcataaat atgccgatag cggtagccat ttttgatgta gggcgcagaa
    20461 acttctcgtt gcggaatgcg gtcgatgatt tcctttaaga tgcgatcctg caaggcttgt
    20521 tgtgaggcca tcacccgatg accgtaacta ttttcttgtt gcaggtagtc caggacttct
    20581 ggctgagaac gcgtatcgtc ccgcagccag tagtaattat cgatgcgcgt atcgccatga
    20641 agcgtcatgg cgtggggaat gcgggcggct tttggtagca tgttattgtt atttctggtt
    20701 gaaacatctt ataagggtgg caaaactcac cggggatgca agcgaaacag gggagtcatt
    20761 gcttagatga tgacaggtaa tggcgcggat atcgaatgtt atgcagacag agaaaatcag
    20821 cctgttcaaa tggctgtgcg attctggata gcccgaaata gtcaacttca ggctatccag
    20881 agagcggaat tattccgcca aagtgcgttt ttgctgttcg agatcgcgtt caatgccgtc
    20941 acgaacatcc tgggggattt tcagcgcgtc acccagtgca ttcaggtaac tgcgttccat
    21001 aaaatggtca atatcaatag ccgcgcaact caggaaatag atttccagcg cctcttcttc
    21061 attgcggacc ccggtagcca ggcgttgtgg atccagcggt tgttcgattg cctgctcaat
    21121 gagtacacgc ccctgctctt ccacgccggc ttcacgcaat tgctggtcga ttgccgcacg
    21181 ttctttggca tcaatatgac catcactttt agcggcaaag accagcgcaa ggatcaaacg
    21241 ttctgtacgc tcatccagcg gcgtactttg cgcgccaaac tgcggttcgt cctgatgcgc
    21301 cgcgcgaatt ttatctttgt atttattcca cagcaccgta cccgctaccg ctccgccgcc
    21361 aaccagtaac gcgttggtgc catatttcgt aagaagttta cgtgctgatt tatttgcgac
    21421 cagcagccca gccagtccgc ctaatgcgcc tggcactaac agtttgccca atccctgatc
    21481 cgcagacgag gaggtagaag aactgctttg cccaagcagg gattgcagtt gatttaacca
    21541 gttagccata tttgctcctc aataaccatt tttagtgcct gtagcatagc ggatgaggat
    21601 gtcagaaaat gtatatcgag acaaaagatg cgcaaataag cggggcggta ggccgttatt
    21661 caaagaaatt cgcgccaggt aaggtattta ctgtatatca ggtgataagg gtattttagg
    21721 tgaaagtagt ttgtcgtttt gagtggcatt gtttgatgct gattgcccga tgcgacgctg
    21781 ttgcgtcttc tcaggcctac gattgtggtg cgttctgggg cggacaaggc actcgcgccg
    21841 catccgccaa cggtcaatcg aattatcaga agccattaac gccgctgata ttccgccatt
    21901 cccgctttgc agtccacgct gacctggtaa tgaatatcgg cacttttacc acgcacggtt
    21961 agcggtactg accatttatc atctttaccc tgaatgtcct gcaaactgac ccaagccacg
    22021 ggatcggcct gaccgacaat tttttgatca tctgcccagc gcgccacgcg attttgttga
    22081 taatcacgtt ttacgctcgc ggcaattccg gcagcatcca gaccttcaca ctttgggaaa
    22141 gtgaccgact tgctggtttc attattggca gcgaaaactg atgcgcaggc agaaaccaac
    22201 aacagcccta aaaacgcccc tctttttttc atgtttttct ccatagcaca atgattcagg
    22261 agaaagcatg gtacaaattg tcaggagcgc aagttgcttc aggccgcgtg tgaggcatct
    22321 tcctcttcgg attcagcacc gacgacctgt ggggacggta atttgccggt tttcagaatg
    22381 gtgctcagca catctttttg ttctgccagc catagcgaaa gggcttcacg ttgctcgtct
    22441 tccatttgca ctggagaatt tgtcagccag gtgtcaagca gatccgccgt atcaagcatt
    22501 ttatcataag catcggcttc ctttttgctg gtaaacgaca ttttctcttc gccctcacga
    22561 atgactacgt atttaacttc aaccgccatt tgcagcctct cataataact gtgattttat
    22621 acagtatatt tcttttcggt tgagaaatca acatcagcaa taaagacaca cgcaaacgtt
    22681 ttcgtttata ctgcgcgcgg aattaatcag gggatattcg ttatgacgtt attaggcact
    22741 gcgctgcgtc cggcagcaac tcgcgtgatg ttattaggct ccggtgaact gggtaaagaa
    22801 gtggcaatcg agtgtcagcg tctcggcgta gaggtgattg ccgtcgatcg ctatgccgac
    22861 gcaccagcca tgcatgtcgc gcatcgctcc catgtcatta atatgcttga tggtgatgca
    22921 ttacgccgtg tggttgaact ggaaaaacca cattatatcg tgccggagat cgaagctatt
    22981 gccaccgata tgctgatcca acttgaagag gaaggactga atgttgtccc ctgcgctcgc
    23041 gcaacgaaat taacgatgaa tcgcgagggt atccgtcgcc tggcggcaga agagctgcag
    23101 ctgcccactt ccacttatcg ttttgccgat agcgaaagcc ttttccgcga ggcggttgcc
    23161 gccattggct atccctgcat tgtaaaaccg gtgatgagct cttccggcaa ggggcagacg
    23221 tttattcgtt ctgcagagca acttgctcag gcatgggaat acgctcagca aggcggtcgc
    23281 gccggagcgg gccgcgtaat tgttgaaggc gtcgttaagt ttgacttcga aattaccctg
    23341 ctaaccgtca gcgcggtgga tggcgtccat ttctgtgcac cagtaggtca tcgccaggaa
    23401 gatggcgact accgtgaatc ctggcaacca cagcaaatga gcccgcttgc ccttgaacgt
    23461 gcgcaggaga ttgcccgtaa agtggtgctg gcactgggcg gttatggtct gtttggtgtc
    23521 gagctgtttg tctgtggtga tgaggtgatt ttcagtgagg tctcccctcg tccacatgat
    23581 accgggatgg tgacgttaat ttctcaagat ctctcagagt ttgccctgca tgtacgtgcc
    23641 ttcctcggac ttcctgttgg cgggatccgt cagtatggtc ctgcagcttc tgccgttatt
    23701 ctgccacaac tgaccagtca gaatgtcacg tttgataatg tgcagaatgc cgtaggcgca
    23761 gatttgcaga ttcgtttatt tggtaagccg gaaattgatg gcagccgtcg tctgggtgtt
    23821 gcgctggcga ccgctgagag tgttgttgac gccattaaac gcgcgaagca cgccgccgga
    23881 caggtaaaag tacagggtta aacccggcaa aaaaacgcta caaaaatgcc cgatccgcga
    23941 tcgggcattt tgacttttac agcttagcgc cttctacagc ttcacgcgcc agcttagtaa
    24001 tacggtcgta atcgcccgct tccagcgcat ctgccggaac cagccaggaa ccaccgatgc
    24061 acagcacgct tttcagcgcc aggtagtcac ggtagttagc cggagaaata ccacctgtcg
    24121 ggcagaaacg gacctgggag aacggacccg cgatcgcctg cagggctttc acgccgccgt
    24181 tagcttcagc tgggaagaat ttgaactctt tcaaaccgta gtccataccc agcatcagtt
    24241 cggaaacagt gctgatcccc ggaatcagag gaatagtccc ttcggtagca gctttcagca
    24301 gcggctcggt cagacccggg ctaattgcga actgtgcacc cgcttcggtg acttctgcca
    24361 gctgctgtgg attcagcacc gtaccggcac ccacaatcgc ttcaggcact tctttggcga
    24421 tagcacggat agcgtcaact gcacactcgg tacgcagagt cacttccaga acgcgcaccc
    24481 caccagcaac caacgctttt gccatcggca ctgcgtgttc cagttttttt accacgataa
    24541 ccggtacaac cgggccggtg gtcaggattg attctgcact tgttttccag tttttcatca
    24601 gagttttctc tcgcctgatt acaaatttgt cgtcttaaaa agtgatacag gttgcgccct
    24661 gttcggcacc ggacagtttt tcacgcaagg cgctgaataa ttcacgtcct gtccccacgc
    24721 gtgacgcgct caggtcagga atgtgcggtt cgcgagcagc cagttccgct tcgtctacca
    24781 gcagcgtcag ttcgcctgtc tgtccattca cacgaatgat gtccccgtcg cgcacttttg
    24841 ccagcagccc gccatcgtag gcttctggtg ttacgtggat agctgacggc actttacctg
    24901 aagcgccgga gagtcgtcca tcggtaacta acgcaatttt gaaacaccgg tccaataata
    24961 caccaagtgg cggcatgagt ttatgtaatt ctggcattcc gttcgctttt ggcccctgat
    25021 gacggacaac aacgacacaa tcgcggtcca gcaaacccgc ttcaaaggcc ggcataacgt
    25081 catgctggct ttcaaaaaca accgctggcg cttcaatcac ctggttctca accggcacgg
    25141 cagaggtttt cataaccgca cggcccaggt taccgcttaa cacttttgtc ccaccatgat
    25201 gagagaaagg ttgttcgaag gaagcgatca cattgctgtc gagtgatttt tccgcccctt
    25261 cccgccagtc cagttcacca ttattcagcc atggttcaag ggtataacga gacagaccaa
    25321 agcctgccac cgtattgaca tcttcatgca gcaggcctgc tttgagcagt tcacgcacca
    25381 gaaccggtac gccacctgcc gcctggaagt ggttaatatc ggccggaccg ttcgggtaga
    25441 gacgtgccat cagcggtaca acatcagaaa ggtcagagaa gtcatcccag ttaatctgaa
    25501 taccggccgc gcgcgccatc gccaccaggt gcatggtgtg gttagtggaa ccaccggtcg
    25561 ccagcagtgc aacgataccg ttcaccacca ctttctcatc gatcatctta ccgatcggca
    25621 tccattcatt accattaccg gtcatgcgtg taacctgacg cgcagcggca gcggtcaaag
    25681 catcgcgcag cggagaatcc ggatgaacaa aagaagagcc tggcaactgc atccccataa
    25741 attccaccac catctggttg gtgttggcag taccgtagaa agtacatgtt cccggcgcat
    25801 ggtaagacgc ggcttctgac tccagtaagg ccatgcggtc cactttacct tcggcataaa
    25861 gctggcgaat acgcactttt tctttatttg gcaaaccact tgccatcggt ccagacggca
    25921 caaacaccgc aggcaaatga ccaaacgaca gggctgccat cgtcagaccc gggacaatct
    25981 tgtcgcacac accgaggaac agagcaccat caaacatgtt atgggacagc ccgaccgccg
    26041 cagacatcgc tatcacttcg cggcttagca gcgacaattc cattccatcc tgcccctggg
    26101 tgacaccatc acacatcgcc ggaacaccgc ccgcaacctg accaaccgca ttcgcttcat
    26161 gcagggcttt acgaatgatt tctggatagt gttcataagg ctggtgcgcg gagagcatgt
    26221 cgttatagga ggtgatgatg gcgatattgt tacgcaacat gcttttcaaa gaggctttgt
    26281 cttctggctg gcaggcagcg aaaccgtgtg ccaggttacc gcatgccaac tgcgaacgat
    26341 gaacagtcga cgttttcgct tgttctatcc gggcgagata agcagagcga gtctcgcgcg
    26401 aacgttcaat gattcgattt gttacgcgta acaattgtgg attcataaag gctcctgaaa
    26461 ttgagttgtc agagcaggat gattcacaac gcgtttcatt cagaggattt atgactgaaa
    26521 cgcctgtaac cggagctcat agggcaaaaa cgtttcagtc agtgtaataa aaaaagcctc
    26581 gtgggtgaat ccgcacgagg cctgaaagtg taaaaattgt tctacaatct gtgcaagatc
    26641 atgttaccgg taaaataacc ataaaggata agcgcagata ttactcaaac tcattccagg
    26701 aacgaccatc acgggtaatc atcgccaccg aggcaacggg tccccaggtt ccggcctgat
    26761 acggtttcgg cgcatcattg tccatcgccc acgcctcagt aatggagtct acccatttcc
    26821 aggcttcttc cacttcgtcg cgacgtacaa acagtgcctg aataccacgc atggtttcca
    26881 gcagcaaacg ttcataggca tccgccagat gcgtctgatt aaaggtttct gaatagctca
    26941 gatccagctt ggtgatttgc aggttatgtt tgtggtcaag gccaggaact ttattcagta
    27001 cctggatatc cacgccttca tcaggttgca gacggatagt cagtttattc tgcggcagat
    27061 cctgccacga ctctttaaac agattcagtt caggtgtttt gaaatagacc acgacttcag
    27121 aacatttggt cggcagacgt ttaccagtac gcaggtagaa tggcacaccg gcccagcgcc
    27181 agttatcaat gtcgacgcgg atcgccacga aggtttctgt attgctgctc ttgttcgcgc
    27241 cctcttcttc cagatatccc ggcacttttt tgccctgggc gaagcccgca gtatattgcc
    27301 cgcgtacggt tttttcgcgt acgttggagc ggtcgatgcg gcgcagagac ttcagtactt
    27361 tcactttttc atcgcggatg ctgtctgcgc tcaggtcaga cggcggagac atcgcaatca
    27421 tgcaaagaat ttgcagcagg tggttctgga tcatgtcgcg catctgaccg gctttatcaa
    27481 aatagcccca gcgcccttcg atccccactt cttctgccac ggtaatctca acatgatcaa
    27541 tggtgcgatt gtcccagtta ttcacaaaca gggagttagc aaaacgcagc gccaacaggt
    27601 tcagcaccgt ttctttacca agatagtggt cgatacggta aacctggcac tcctcgaagt
    27661 attcgccaac ctgatcattg atttcctgcg aggtcgccag cgacgtcccc agcggtttct
    27721 ccatgactac gcgtgccggt ttagcattca gtttagcctc gccaagccct ttgcaaattg
    27781 cgccaaaagt gctgggcggc atggcaaagt agttaatggt gatacgattt ttttgatcca
    27841 gcatcgcgcc gagacggctg aatgcagcag tgtcattgac atcgagatta caaaaatcca
    27901 gacgtgcact cagggtgtcc cataaacctt catcaatggt ttctttcatg aaagtttcga
    27961 gcgcctcgcg gacaactttg gtatatgccg ctttatccca gtcagcacgc cctacgccga
    28021 taatccgggt gtccgggttg agctgaccgg ctttttccag ttgatacagg gaaggcagca
    28081 atttacgacg cgcaaggtcg cctttcgcgc cgaaaatgac caggtcacag gcctgggctg
    28141 tttgcgttac cgccatgtca ttctccttaa gttaactaac ccggtactta agccagggta
    28201 tacttgtaat tttcttacgg tgcactgtac tgcttttacg agcttgcgaa aactgtaaac
    28261 gcttatccac ccgtgcgatt acgggaaaag cgcgcaaagt gcggcaaaac tgataaaaaa
    28321 atcatcgttt tccattgggt aaaaatctga cactgatcat gttatgaaaa aaaataacaa
    28381 cttttttatc tgcttttgtc attaacacgg cacacaggcg taatatctga caaaactgca
    28441 tttcgatttc tttcagtgcg gaaatcgtca ttacccgtga gtctctttac atcatgaata
    28501 tgctggaaaa aatccagtct cagctggaac atttgagcaa atcagagcgc aaagttgccg
    28561 aggtcattct ggcttcgccc gataacgcga tccattcgag tattgctgct atggcactgg
    28621 aagccaatgt tagcgaaccg acggtgaatc gtttctgtcg cagcatggac acgcgcggtt
    28681 ttcctgattt taaacttcat ctggcacaga gtctggcgaa tggcactccc tatgttaatc
    28741 gcaatgtcaa tgaagatgac agcgttgaat catacacagg gaaaatattt gagtccgcaa
    28801 tggcaacgct tgatcatgtc cgtcattcac tggataaatc tgccatcaac cgcgccgtcg
    28861 acttgctcac tcaggcaaag aaaatcgcct ttttcggatt aggctcttca gccgccgttg
    28921 cccacgatgc gatgaataag ttctttcgtt ttaatgttcc ggtggtgtac tccgatgata
    28981 tcgtgctgca acgcatgagt tgtatgaatt gtagcgacgg agacgtggtg gtgctgattt
    29041 ctcacactgg aagaacaaaa aatctggtcg agctggcgca gctggcacgc gaaaacgacg
    29101 ccatggtgat tgccctcacc tctgcgggta ccccgctcgc ccgggaagca acgctggcaa
    29161 ttaccctcga cgtaccggaa gatactgaca tttatatgcc catggtttct cgacttgcac
    29221 agctgaccgt gatagatgtg ctggcgacag gatttacttt gcgacgcggt gcaaaattca
    29281 gagataactt gaagcgggtc aaggaagcgc tgaaggaatc gcgttttgat aagcagttac
    29341 ttaatttaag tgacgatcgc taaaaacgac tgtcactgtc ctaatcttat acgacatccg
    29401 aatgagatta atttatcgcc atcgcggcgt tatttcattc ggatttcatg ttcaagcaac
    29461 acctggttgt ttcagtcaac ggagtattac atgtccagaa ggcttcgcag aacaaaaatc
    29521 gttaccacgt taggcccagc aacagatcgc gataataatc ttgaaaaagt tatcgcggcg
    29581 ggtgccaacg ttgtacgtat gaacttttct cacggctcgc ctgaagatca caaaatgcgc
    29641 gcggataaag ttcgtgagat tgccgcaaaa ctggggcgtc atgtggctat tctgggtgac
    29701 ctccaggggc ccaaaatccg tgtatccacc tttaaagaag gcaaagtttt cctcaatatt
    29761 ggggataaat tcctgctcga cgccaacctg ggtaaaggtg aaggcgacaa agaaaaagtc
    29821 ggtatcgact acaaaggcct gcctgctgac gtcgtgcctg gtgacatcct gctgctggac
    29881 gatggtcgcg tccagttaaa agtactggaa gttcagggca tgaaagtgtt caccgaagtc
    29941 accgtcggtg gtcccctctc caacaataaa ggtatcaaca aacttggcgg cggtttgtcg
    30001 gctgaagcgc tgaccgaaaa agacaaagca gacattaaga ctgcggcgtt gattggcgta
    30061 gattacctgg ctgtctcctt cccacgctgt ggcgaagatc tgaactatgc ccgtcgcctg
    30121 gcacgcgatg caggatgtga tgcgaaaatt gttgccaagg ttgaacgtgc ggaagccgtt
    30181 tgcagccagg atgcaatgga tgacatcatc ctcgcctctg acgtggtaat ggttgcacgt
    30241 ggcgacctcg gtgtggaaat tggcgacccg gaactggtcg gcattcagaa agcgttgatc
    30301 cgtcgtgcgc gtcagctaaa ccgagcggta atcacggcga cccagatgat ggagtcaatg
    30361 attactaacc cgatgccgac gcgtgcagaa gtcatggacg tagcaaacgc cgttctggat
    30421 ggtactgacg ctgtgatgct gtctgcagaa actgccgctg ggcagtatcc gtcagaaacc
    30481 gttgcagcca tggcgcgcgt ttgcctgggt gcggaaaaaa tcccgagcat caacgtttct
    30541 aaacaccgtc tggacgttca gttcgacaat gtggaagaag ctattgccat gtcagcaatg
    30601 tacgcagcta accacctgaa aggcgttacg gcgatcatca ccatgaccga atcgggtcgt
    30661 accgcgctga tgacctcccg tatcagctct ggtctgccaa ttttcgccat gtcgcgccat
    30721 gaacgtacgc tgaacctgac tgctctctat cgtggcgtta cgccggtgca ctttgatagc
    30781 gctaatgacg gcgtagcagc tgccagcgaa gcggttaatc tgctgcgcga taaaggttac
    30841 ttgatgtctg gtgacctggt gattgtcacc cagggcgacg tgatgagtac cgtgggttct
    30901 actaatacca cgcgtatttt aacggtagag taagtacgtt gccggatgcg gcgaaaacgc
    30961 cacatccggc ctacagttca atgatagttc aacagatttc gaatattctg aagcaaactt
    31021 gaacttatca tcaggcgaag gcctctcctc gcgagaggct tttttatttg atgggataaa
    31081 gatctttgcg cttatacggc tggatttcgc ccggtttgcg agttttcagc aattttagta
    31141 tccaggtgta ttgttctggt cgcggaccaa caaaaatctc gacttcttca ttcatccgcc
    31201 gcgcaatcgt atgatcatcc gcctctaaca gatcatccat cggtgggcgc acctgaatcg
    31261 tcagacgatg cgtcttgcca tcataaatcg gaaacagcgg tacaacgcgc gcacggcaca
    31321 ctttcatcaa acgaccaatc gcgggcaacg tcgctttata ggtggcaaag aaatccacaa
    31381 attcgctgtg ctctgggcca tgatcctgat cgggtaaata atatccccag tacccctgac
    31441 gtaccgactg gatgaatggt ttaataccgt catttctcgc atgcagacga ccgccaaagc
    31501 gacgacgcac cgtgttccag acataatcaa aaaccgggtt gccctgatta tggaacatcg
    31561 ctgccatttt ctgcccttgc gaggccatca gcatggcagg aatatcgacg gcccaaccgt
    31621 gcggcaccag aaagataact ttctcgttat tacgccgcat ctcttcgatg atctccagcc
    31681 cttgccagtc aacgcgcggc tgaattttct ccggcccgcg tattgccaac tcagccatca
    31741 ttgccatcgc ttgcggcgcg gtggcaaaca tctcatcaac aatcgcttcg cgttcagctt
    31801 cactacgttc tggaaagcag agcgacagat tgattaacgc acgacggcgt gagctttttc
    31861 ccagtcgtcc ggcaaaacgt cccagccgtg ccagaatggg atcacggaac tttggcggcg
    31921 ttaaagcgat acccgccatc gctgctacgc ccagccatgc tccccagtag cgcgggtggc
    31981 gaaaggattt atcaaactca ggaatgtatt cgctattatt ttttttcgtt tccatgcttt
    32041 tccagtttcg gataaggcaa aaatcaatct ggtgatagtg tagcggcgca acttgccccg
    32101 caccaaataa aaaaagccgg tactgactgc gtaccggctg cgaatggatg ttaattaatc
    32161 aaaccgtagc tgcggcacaa tctctttggc ctgcgccagg aattcgcgac gatcggagcc
    32221 ggtcagccct tcggtacgcg gcagttttgc cgtcagcggg tttacggcct gctggtttat
    32281 ccatacttca tagtgcagat gcggcccggt tgaacgtccg gtattaccgg aaagcgcgat
    32341 acggtcgcca cgtttcacct tctgtcccgg tttcaccaga atcttgcgca agtgcatata
    32401 acgcgtggtg tagctgcgac catgacgaat agccacataa taacctgctg cgccactgcg
    32461 tttggcaacc accacttcac cgtcacccac tgaaagcact ggcgtaccct gcggcatggc
    32521 gaaatcaaca cctctgtgtg gtgcaacgcg accggtcacc ggattagtac gacgcgggtt
    32581 aaagttagac gagatacgga actgtttcgc cgtcgggaat cgcaagaatc ctttcgccag
    32641 accagtaccg ttacgatcgt agaatttgcc atcttcagcg cggatagcgt aataatcttt
    32701 accttctgaa cgcaaacgta cgcccagcag ttggctttgc tcacgtttac catcaagcat
    32761 ttctcgagac attaacaccg caaattcatc gccttttttc agtttgcgga aatccatttg
    32821 ccactgcatg gctttaatca ctgcgctcac ttcggcgctg gttaaaccgg cgtttctggc
    32881 gctggcaaca aagcttcccc cgacggtacc tttcagcaga ttgttaaccc actctccttg
    32941 ctgcatttcg ctggtcattt taaaaccgtt agcggcagta cggtcatagg ttcgggtttc
    33001 acgacgagac acttcccagg tgaggcgctg cagttcgccg tccgcggtta atgtccagga
    33061 gagttgttga ccgattttca ggttacgcaa ttctttgtcg gccgcagcca gttgggtgat
    33121 atcacccata tcaataccat actgattgag aatgctgctt agcgtatcgc cagtggaaac
    33181 aacatattca tgcacgcccg cttcaccggc gattttgtca tccagttcgt cctggggaat
    33241 ggcttcatct tcttgtgcag cttgatcaat cggctcactg gcttcaggta agagcgaacg
    33301 aatttcgttc tgttccagct caatggtttt gacaattggc gtggcatcac ggtgataaac
    33361 ataaggccgc cagacagcga cggccagagt aagaacggtg agcgacccca acataacgcg
    33421 gtgtggtcgc ggtaaattat taaacgccag ggcgacagag cgggctatct gttgcacgta
    33481 atcacttcct cattaatctc ctttcaggca gctcgcatac tggttggcta attgactcag
    33541 gaattctgaa tagcttgttt tacccagttt gatattcgtc cccaggggat ccaacgttcc
    33601 catacgaacg gatgtccctc gtgcgacgct ctcaacgacc gctggcctga actgtggctc
    33661 agcaaaaacg caggttgctt tttgctcaac caactgtgtt cttatttcat gtaaacgctg
    33721 cgcgccaggt tgaatctcag ggttaacggt aaaatgacca agcggtgtca gtccaaactg
    33781 tttttcgaaa tagccgtaag catcgtgaaa aacgaaataa cctttcccct tgagcggcgc
    33841 gagctcgtta ccaacctgcg tttcggttga ggctaattgt gcctcaaaat ccttcaggtt
    33901 ggcgtcaagt ttggctcgac tttgcggcat aagttccact aattttccat ggattgcaac
    33961 cgctgtagcc cgcgctatct ctggggaaag ccaaagatgc atgttgaaat cgccgtgatg
    34021 gtgatcttcg tcactttttt ccgcgtggtc gtgatcatca tcatcgccgt gaatactttt
    34081 catcagtagc ggtttcacat cttcaagctg cgcaatcgtt acctgcttcg cttctggtaa
    34141 tttgcttacc ggtttttgca taaacgcttc catctccggg ccaacccaaa cgactaagtc
    34201 cgcgttctgt aagcgtttta catccgatgg acgcagtgaa taatcatgtt cagaagcgcc
    34261 gtcaggcagt aaaacctccg tttctgttac accatcagca atggcagaag cgatgaaccc
    34321 aacgggttta agcgaagcga caacggcggc atctgcggcc tgtgttgcac ctccccagag
    34381 agcggcggat aatgctgcga aaagaagcgt ttttttatgt aacataatgc gaccaatcat
    34441 cgtaatgaat atgagaagtg tgatattata acatttcatg actactgcaa gactaaaatt
    34501 aacatgacaa gtctggtttc cctggaaaat gtctcggttt cttttggcca acgccgcgtc
    34561 ctctctgatg tgtcgctgga acttaaacct ggaaaaattt tgactttact tgggccaaat
    34621 ggcgcaggta agtcgacact ggtacgggta gtgctcgggc tggtaacacc cgatgaaggg
    34681 gttatcaagc gcaacggaaa actacgcatc ggctatgtac cgcagaagct gtatctcgac
    34741 accacgttgc cactgaccgt aaaccgtttt ttacgcttac gccctggcac acataaagaa
    34801 gatattttgc ctgcactgaa acgtgtccag gccggccacc tgattaacgc accgatgcaa
    34861 aagctctcgg gtggcgaaac gcagcgtgta ctgttagcgc gagcattgtt aaatcgcccg
    34921 caattattag tgctggatga acccactcaa ggcgtggatg taaatggcca ggtggcgtta
    34981 tatgacctta ttgaccagtt gcgtcgcgaa ctggattgtg gcgttttaat ggtttctcac
    35041 gatctgcatc tggtgatggc aaaaaccgat gaagtgctgt gcctgaatca ccacatttgt
    35101 tgttccggca caccggaagt tgtttccctg catccggagt ttatttcaat gtttggtcct
    35161 cgtggtgctg aacaactggg tatctatcgc catcatcata atcatcgtca cgatttacag
    35221 ggacgaattg ttttgcgtcg gggaaatgat cgctcatgat tgaattatta tttcccggtt
    35281 ggttagccgg gatcatgctc gcctgtgccg cgggtccgct gggttcgttt gtagtctggc
    35341 gtcgtatgtc ttatttcggt gatacgctgg ctcatgcctc attacttggc gtcgcgtttg
    35401 gtttgttgct ggacgtgaat ccattctatg cggtgattgc cgttacgctg ctgctggcgg
    35461 gcggtctggt atggctggag aagcgtccac agctggcgat cgacacgtta ttagggatta
    35521 tggcgcacag tgccctgtcg ctgggcctgg tggtcgttag tctgatgtct aatattcgtg
    35581 ttgatttgat ggcttacttg ttcggtgatt tgctggcagt gacgccggaa gatctcatct
    35641 ccatcgccat tggcgtggtc atcgtggtgg ctattttgtt ctggcaatgg cgcaatttgc
    35701 tgtcgatgac gattagcccg gatctggcgt ttgttgatgg tgtgaaatta cagcgcgtga
    35761 aattgttatt gatgctggtg acggcattga cgattggtgt agcgatgaaa tttgtcggtg
    35821 cgttgattat tacttcgttg ctgattattc ctgcagctac tgcgcgtcgc tttgcccgca
    35881 cgccggaaca gatggctggt gtcgctgttt tggtggggat ggtggcagtg actggcggtt
    35941 taaccttttc cgcattttac gatacgccgg cgggtccgtc ggtggtgcta tgtgcggcac
    36001 tgttatttat tctcagtatg atgaaaaagc aggccagcta atctatcgct gaacacattt
    36061 gtcggatgcg gcgcgagcgc cttatccgac ctacggttcg ctatctctgg ctggcctgat
    36121 aagacgcgaa cagcgtcgca tcaggcacac tgccaatgtc ggatgcggtg cgagcgcctt
    36181 atctgaccta cggttcgcta tctctcgtag gccggataag acgcgaatag cgtcgcatca
    36241 ggcacactgc caatgtcgga tgcgtcgcga gcgccttatc cgagctacgg ttcgctatct
    36301 ctggtaggcc ggataagacg cgaatagcgt cgcatcaggc acactgccaa tttcggatgc
    36361 ggtgcgagcg accaatccga cttacggcat ttctggcggc gttatgccaa agtgattcca
    36421 cgcccgcgtc gtcgccatac gcccacgcgg tgtacgctgc aaaaagcctt gctgaatcaa
    36481 ataaggttcc agcacatcct caatggtttc acgttcttcg ccaatggctg ccgccaggtt
    36541 atccagacct acaggtccac caaagaactt atcgattacc gccagcaaca atttgcggtc
    36601 catataatcg aaaccttcag catcgacatt caacatatcc agcgcctgag cagcgatatc
    36661 tgccgagatg gtgccatcgt gcttcacttc ggcgaaatca cgcactcgac gcagcagacg
    36721 gttggcaatg cgcggcgtac cgcgagcgcg acgagcaact tccagcgcgc cgtcatcact
    36781 catctcaagc cccataaagc gtgcgctgcg actgacgata tattgcagat ccggcacctg
    36841 ataaaactcc agacgttgca caataccaaa acggtcgcgc aacggtgatg tcagcgaacc
    36901 tgcgcgcgtg gttgcaccaa tcagggtaaa cggcggcaaa tcaattttaa tggagcgtgc
    36961 cgccggacct tcaccaatca tgatatccag ttggtagtct tccattgccg ggtacagcac
    37021 ttcttcaaca actggcgata gacggtggat ctcatcaata aacagcacgt catgcggttc
    37081 aaggttagtg agcatcgcag ccaaatcgcc cgccttttcc agcaccggac cagaagtcgt
    37141 gcgtaaatta acgcccattt cattggcgac aatgttggca agcgtagttt tacccaaccc
    37201 cggaggacca aaaatcaaca aatgatcgag ggcatcgccg cgcagtttcg ctgctttgat
    37261 gaaaatctcc atctgtgaac gaacctgcgg ctgaccaaca tactcttcca gtaatttggg
    37321 gcgaatggcg cgatctgcta catcttccgg caaagtggta ccggcagaaa tcagacggtc
    37381 tgcttcaatc atcctttacc tcataacgcg gcgcgtaggg cttcgcgaat taatgtttca
    37441 ctgctggcgt cagggcgagc gattttgctc accatgcggc ttgcttcttg tggtttatag
    37501 cccagcgcca ccagcgcggc aaccgcttcc tgttcagcat cgttggttgc cgggctggca
    37561 ggcgacgtga gcaccagatc ggcggctggc gtaaagagat cgccatgcag acctttaaat
    37621 cggtctttca tttcaacaat caagcgttcg gcggtttttt tgccaatacc cggcagtttc
    37681 accagcgccc ccacttcttc acgctcaacg gcattaacga actgctgcgc tgacattccg
    37741 gagaggatcg ccagcgccaa cttcgggccg acgccgttgg ttttgatcaa ctctttgaac
    37801 aacgtgcgct cttgtttatt gttaaaaccg tacagcagct gcgcgtcttc acgcaccaca
    37861 aagtgggtga aaacgatcgc ttcctgtccc gcttcaggga gttcataaaa acaggtcatc
    37921 ggcatatgca cttcatagcc tacgccgccc acttcaatta acaccagcgg gggttgtttt
    37981 tcaatgatga tgcctctgag tctgcctatc acatgacgct cctgcgtaat gaatcaaaga
    38041 taatgctgta tgataaaaaa atgctggata gatatccagc gaaggatgaa gaaaacttgc
    38101 gaggtgtctc gatgatctga aaaatggcac agtataattt attctacagg ttattttgga
    38161 agcaaatatt ttaatattac atattcagtg aaaaaatgcg taataaaaat atacattgcg
    38221 cctcctgaaa aaataaattt tttatgctat tacgtatatt cgtatctatt tcaatggaat
    38281 gacaacgtga atattaatta ccctgctgaa tatgaaattg gagatatcgc ctttacatgt
    38341 ataggtgctg ccttatttgg tcaaatatca gctgcatcaa attgttggag taatcacgtc
    38401 gggatcatta tcggtcataa cggtgaagac ttcctggttg cagaaagccg ggttcccctc
    38461 tcaaccatca ctacgctatc ccgttttatt aaacgctctg ctaatcaacg ctatgctata
    38521 aagcgattag acgctgggct gacagaacaa caaaaacaac gaattgttga acaggttccc
    38581 tcccggctac gcaaacttta ccacaccggt tttaaatacg aatcttcgcg ccagttctgt
    38641 tcaaaatttg tctttgatat ttataaagag gcgctatgta ttccggtggg tgaaatagag
    38701 acgtttggag aattgttaaa tagcaatccg aatgcaaaac tcactttctg gaaattctgg
    38761 ttcctgggtt ctattccgtg ggagcgtaaa accgttacgc cagccagttt atggcatcat
    38821 ccgggtttgg tgttgattca tgcggaggga gtggaaacgc ctcagccgga actgaccgag
    38881 gcggtataac ttaacgcagt cgccctctcg ccaggttcag ccgcgattcg ctcatctgca
    38941 tcgcattctg actaacgtgg cagtgggtga tggcaatcgc caacgcatca gcggcatccg
    39001 cctgtggatt agcgggcagt ttcagcaagg tgcggaccat atgctgcacc tggctttttt
    39061 cggcgctacc aatacccacc accgtttgct tcacctgacg tgcagcatat tcaaataccg
    39121 gcaactcctg attcaccgcc gccacaatcg ccacgccgcg cgcctggccc agtttcaggg
    39181 ctgagtcagc gttctttgcc ataaagactt gttcaatggc gaaataatca ggctggaact
    39241 gggtgatgat ttccgtcacg cccgcataga tgagtttcag acgagacggt aaatcatcca
    39301 ctttggtgcg tatgcatccg ctacccaggt aggacagttg cctacctacc tggcggatga
    39361 cgccgtagcc ggtcacgcgc gaacccggat caatgccgag aataatagcc atcacgcgtc
    39421 tccgttttgc tgtttagcag gcctcatcag agagtcgctg cgacctcatc agagatttca
    39481 ccgttatggt aaacttcctg cacgtcgtcg caatcttcca gcatatcgat cagacgcatc
    39541 agtttcggtg cggtttctgc atccatatca gctttggtag acgggatcat ggaaacttcc
    39601 gcgctgtctg ctttcagacc tgccgcttcc agagcgtcgc gcactttacc catttcttcc
    39661 catgcagtgt agacatcaat cgcgccgtca tcataggtca caacgtcttc agcacctgct
    39721 tccagtgctg cttccatgat ggtgtcttca tcgcctttct cgaaggagat cacgcctttt
    39781 ttgctgaaca gataagctac ggaaccatca gtaccgaggt taccgccaca tttgctgaat
    39841 gcatgacgca cttcagcaac ggtacggttg cggttgtcag acagacattc aatcatgatt
    39901 gccgtgccgc caggaccgta accttcgtag atgatggttt ccatgtttgc atcatcatca
    39961 ccgcccacac cacgtgcaat tgcgcggttc agtgtgtcac gggtcatgtt gttagacagt
    40021 gctttatcca ccgccgcacg cagacgcggg ttagcgtccg gatcgccacc gcccagctta
    40081 gccgcggtta ccagctcacg aatgatttta gtgaagattt taccgcgctt agcatcctgc
    40141 gcagctttac gatgtctggt gttggcccat ttactatgac ctgccataaa aatatctcca
    40201 gatagccctg cctgttcagg cagcgttaat tacaaactgt tcaatcgcct gccggttgct
    40261 ccaggactta gtgagcgccg ccgcagcagg cgcatcaagc cacttgtaag ccagatgttc
    40321 agtgaaaacg atctgccgct cgtgcggaag cgcaagacag aaccatgatt ccgtattaca
    40381 cgtcacgccc ggcgcatagc gatgacgtaa atgtgaaaaa atttcaaact ctaccgtgcg
    40441 ctgacagtca attaaggtca gttgttcagc gacaacatca atggtgacct cttcctttac
    40501 ttcgcgcatg gcagcttgcg gcgcggtttc accctcttcc acgctgccgg ttaccgactg
    40561 ccagaaatcg ggatcgtcac gccgctgcaa catcagcacc cgtttcgtat cttgtgcgta
    40621 gatgaccact aagatcgaaa cgggacgctt atacacttta tccttcacgc tgcctcttcg
    40681 ttgactgcct tcgctcatcc cattcacata gttatctatg ctcatgggag ttcactcagt
    40741 tgccgcctcg atgcaacgcg aatgatttcg tgtatttgag tcatatcagt tattctcagc
    40801 cttcttcaca acctgaatgc tcagctcagc cagtgcagtc gggttagcaa agctcggtgc
    40861 ttcagtcatc agacacgctg ccgccgtggt tttcgggaag gcgataacgt cacggatatt
    40921 gtcggtgccg gtcagcagca tggtcagacg gtcaagaccg aatgccagac ctgcgtgcgg
    40981 cggagtaccg tatttcagag cgtcgagcag gaagccgaat ttctcgcgct gttcctcttc
    41041 gttgataccc agaataccaa acaccgtctg ctgcatatca ccattatgga tacgtactga
    41101 accaccgccc acttcgtaac cattgatgac catatcgtaa gcgttcgcca ccgcattttc
    41161 cggtgcagct ttcagttctg cagccgtcat atctttcggt gaggtgaacg gatggtgcat
    41221 tgccgtcagg ccgccttcac cgtcgtcttc aaacatcggg aagtcgataa cccacagcgg
    41281 tgcccatttg ctttcgtcgg tcagaccaag gtctttaccc actttcaggc gcagtgcacc
    41341 catcgcgtcg gcaacaattt tcttgttgtc ggcaccgaag aaaatcatat cgccatcttg
    41401 cgcggcagta cgatccagga tgtcttcgat gatttctgca ttaaggaact tcgctaccgg
    41461 gctgttgata ccttccagac ctttcgcgcg ttcgttaact ttgatgtaag ccagaccttt
    41521 cgcgccgtag attttaacga agttaccgta ttcgtcgatc tgcttacggg tcagcgatgc
    41581 gccgcccgga acgcgcagag ccgctacgcg acctttcgga tcgttcgccg gacctgcaaa
    41641 tacagcaaac tcaacagatt tcagcagatc agcaacgtca gtcagttcca tcgggttacg
    41701 cagatccggt ttatcagaac cataacggcg ttctgcttcc gcaaaggtca ttaccgggaa
    41761 atcgcccaga tccacaccct tcacttccag ccacagatga cgcaccagcg cttccatcac
    41821 ttcacgcact tgcggcgcgg tcatgaaaga agtttccaca tcgatctgag taaattcagg
    41881 ctgacggtca gcacgcaggt cttcgtcacg gaagcattta acgatctgat agtaacggtc
    41941 aaaaccggac atcatcagca gctgtttgaa caactgcggg gattgcggca gtgcgtagaa
    42001 tttacctttg tgcacacgag aaggcaccag gtagtcacgc gcgccttccg gcgtggcttt
    42061 ggtcagcatc ggagtttcga tgtcgaggaa gccgtggtca tccataaaac ggcgcaccag
    42121 gctggtgatt ttagcgcggg ttttcaggcg ctgagccatt tccggacgac gcaggtcgag
    42181 gtagcggtat ttcagacgcg cttcttcggt gttgacgtgg ttagagtcaa gcggcagaac
    42241 atctgcgcgg ttgatgatag tcagcgagga cgccagcact tcgatttcgc cggtcgccat
    42301 atcgcggtta atattttttt cgtcacgcgc acgtacggtg cccgtgacct gaatgcagaa
    42361 ctcattacgc agttcagagg ccagctttaa cgcgtccgca cgatccggat cgaaaaatac
    42421 ctgcacgata ccttcgcggt cgcgcatatc gatgaagatc aggctaccaa gatcacgacg
    42481 acggttgacc caaccacaca gagtcacctg ctgccccacg tgggacaaac ggagctgtcc
    42541 acaatattct gtacgcatga gatatccctt aacttagctg ccggcggatg ccccctgctg
    42601 cgcaggtgac caagtcgcag cgttagctgt atgtcacaac tgaatgaaaa aaggcggcta
    42661 ttatactgga aattctgccg caccgtaaga gcctggcccg cgctggaacg cctcgttacc
    42721 actttatatc gggcctgaaa tcagactcta cgccagtttg ctataaaggt gttgcccgaa
    42781 ctcataaaaa ttaacaaaat ttgtcgttcc gccatcggct aatcgcatta aggtgtgagg
    42841 cacgattttg ttttttcagg agtcattatg cttgaactta atgctaaaac cactgcgctg
    42901 gtggtgatcg atttacaaga aggcatctta ccttttgccg gtggtccaca tactgccgat
    42961 gaggtagtta atcgcgccgg gaagctggcg gcgaaatttc gcgccagcgg tcagcccgtg
    43021 tttctggtgc gcgttggctg gtctgccgat tacgctgaag cattaaaaca gccggtcgat
    43081 gcgccctcac cggctaaagt gttgcccgaa aattggtggc aacatcctgc tgcattaggt
    43141 gcaaccgaca gcgatatcga aatcatcaaa cgtcaatggg gtgcgtttta cggtacggat
    43201 ctggagttgc aattacgccg ccgaggtatc gatacaatag tgttatgtgg gatctcgacc
    43261 aatatcggtg ttgaatccac cgcccgcaat gcctgggaac ttggctttaa tctggtgatt
    43321 gccgaagacg cctgtagtgc cgccagcgcc gagcagcaca ataacagtat caaccatatc
    43381 tacccgcgca tcgcccgtgt gcgtagcgtg gaagagatcc tcaacgcgtt atgatttaca
    43441 tcggtctacc gcaatggtcg catcctaaat gggtgcggtt ggggatcacc agccttgaag
    43501 agtatgcccg ccactttaac tgcgtggagg gcaacaccac gctttacgcc ctgccgaaac
    43561 ccgaggttgt cctgcgctgg cgtgagcaga ccacagatga cttccgcttc tgttttaagt
    43621 ttccggcgac catttcgcat caggcagcat tacggcattg cgatgattta gtgactgaat
    43681 ttttgacccg catgtcaccg ttggctccgc gcattgggca atactggctg caactgcctg
    43741 ccacattcgg cccacgggag ctgcctgcgc tttggcattt tctcgattct cttcctggcg
    43801 aatttaatta tggcgtggaa gtccgccatc cacagttttt cgccaaagga gaagaggaac
    43861 aaacgcttaa tcgcggttta catcagcgcg gcgttaatcg ggtgatttta gacagccgcc
    43921 cggttcatgc agcacgtcca cacagtgaag ctattcgcga cgctcaacga aaaaaaccta
    43981 aagttccggt acatgctgta ctgacggcga caaatccgct gatccgtttt atcggtagtg
    44041 atgatatgac gcaaaaccgt gaattatttc aggtctggtt acaaaaatta gcgcagtggc
    44101 atcagaccac tacgccttat ctttttttac atacgccaga tattgcccag gccccggaac
    44161 tggtacatac cctgtgggaa gacttacgta aaacgcttcc agagatcgga gcagttccgg
    44221 ctattccaca gcaatcttct cttttctgaa tttgccacct atcatagaca agtgccatcg
    44281 gccattttaa agggagtttg tatggtaagc gcgctgtatg ccgttttaag tgcgttgtta
    44341 ttaatgaagt tctcttttga tgtcgttcgc ctgcgaatgc agtaccgcgt tgcctatggt
    44401 gacggtggtt ttagcgaact gcaaagcgct attcgcattc atggtaacgc ggtggaatat
    44461 attcccatcg cgattgtgct gatgctgttt atggaaatga atggcgcaga aacctggatg
    44521 gtgcatattt gcggcatcgt tttgcttgct ggtcgtctga tgcattatta cggttttcat
    44581 caccgtctgt ttcgctggcg acgttccggc atgagcgcca cctggtgtgc gctgttgctg
    44641 atggtgctgg cgaatctttg gtatatgccc tgggagttgg ttttctccct gcgttagcgc
    44701 acaatacgcc actttctttt tcccggattt ttacgttatg tctcaccgcg acacgctatt
    44761 ttctgcccct atcgccagac tgggcgactg gacctttgat gaacgggtag ctgaagtctt
    44821 cccggatatg atccagcgtt ccgttcccgg ctattccaat attatttcca tgattggtat
    44881 gttagccgag cgcttcgttc aacctggtac gcaggtttac gatctgggtt gttctctggg
    44941 cgcggcgacg ctctcggtgc gtcgcaacat tcatcatgat aattgcaaaa ttattgccat
    45001 cgacaactcc ccggcgatga ttgaacgctg ccgtcgtcat attgacgcct ataaagcccc
    45061 tacgccagta gacgtcattg aaggtgatat tcgcgatatc gccattgaaa acgcatcgat
    45121 ggtggtgctg aattttaccc tgcaattcct ggaaccttcc gagcgccagg cgttactgga
    45181 taaaatttat caagggctga accccggcgg cgcgctggtg ctttcggaaa aattcagttt
    45241 cgaagatgcc aaagttggtg aactgttgtt caacatgcac cacgacttta aacgtgccaa
    45301 cggttacagc gaactggaga tcagccagaa gcgcagcatg ctggaaaacg tgatgctgac
    45361 cgattccgtg gaaacccata aagcacgcct gcataacgcc ggttttgagc atagtgagct
    45421 gtggttccag tgctttaact ttggttcact ggtggcatta aaagcagagg acgctgcatg
    45481 atcgactttg gtaactttta ttctctgatt gccaaaaatc atctttcaca ctggctcgaa
    45541 acgctgcccg cgcagattgc taactggcag cgcgagcagc agcacgggtt gtttaagcag
    45601 tggtcaaatg cggtggagtt tctacctgaa attaaaccgt atcgtcttga tttattgcat
    45661 agcgttacgg cagaaagcga agagccactg agcgcagggc aaattaaacg cattgaaacg
    45721 ctgatgcgca acctgatgcc gtggcgcaaa gggccgttct cactgtatgg cgtcaacatc
    45781 gataccgaat ggcgttccga ctggaaatgg gatcgtgttc tgccccatct ttctgattta
    45841 accggacgca ccattctgga tgtcggctgc ggcagcggtt atcacatgtg gcgcatgatt
    45901 ggcgcaggcg cgcacctcgc ggtggggatc gaccctacgc aactgtttct gtgccagttt
    45961 gaagcggtgc gtaaactact gggtaatgat cagcgcgcgc atttgttacc gttaggtatt
    46021 gaacaacttc cggcactgaa agcctttgat accgtctttt cgatgggcgt gctttaccat
    46081 cgccgttcac cgctggagca tctatggcag ttgaaagatc aactggtcaa tgaaggtgaa
    46141 ctggtgctgg aaacgctggt tattgatggt gatgagaaca cggtgctggt accaggcgat
    46201 cgttacgcgc aaatgcgtaa tgtctatttc atcccttccg cgctggcgct gaaaaactgg
    46261 ctgaagaagt gtggttttgt agatatccgc attgcggatg tgagcgttac caccacagaa
    46321 gagcagcgac gcaccgaatg gatggttacc gaatcgctgg ccgatttcct cgacccacat
    46381 gatccgggta aaacggtgga aggttatccg gcacctaaac gcgcggtgct gattgcgcgc
    46441 aagccgtaaa ggtctggtat tactgccgga tgcggcgtga acgccttatc cggcctacaa
    46501 aaccttgcta attcaatata ttgcagggac tatgtaggcc tgataagcat agcgcatcag
    46561 gcagctttac gtttgcataa cctcagcgcc cgtttccggg cgctattcac gtcttacgcc
    46621 tgaacagctg gctgatcaaa cgccgttaac ttcggtgcat tgcccgtata tttttcgata
    46681 tacaccaacg ctgagttacc ggcacaggca tttgccagct gcgagctggg gatatccgcc
    46741 gttaacacgt tcgcactgcc gtttttacac aagccatttt ccagatctgg ccatgcacct
    46801 tcatgcacgc ataccacgcc ttttttgatc ccgtcagtca ccaccgcgcc tgtcaaaatc
    46861 tgaccacgtt tgttccagac acgcaccaga tcgccattcg caataccaaa gcgtgcagca
    46921 tcttcggtgt gaatagtgat tggttcacga tcggcgatcg catatttttt acgcagttcc
    46981 gcatagttaa gctgactgtg taaacggtgt gccggatgtg cggtcagaag ctgtaactgc
    47041 ttctcgtcgg cggtaccttt ccattcatca ggagccagcc aggttgggtg tgccgggcaa
    47101 tccttatagc caaatttttc caacgttttt gaatagattt caattttgcc gcttggcgta
    47161 cccagcgcat ttttcaccgg atcggcgcgg aaatcaccat aacgaacgta ctgttcgttc
    47221 ttctcgctgc ggcgcatttc gatcagttta ttttgctgcc agaaggcatt aaacattggc
    47281 atagtgacgc gttgcgcacg ggcacctttc tgagcggcat catagaaaaa tttcagccac
    47341 gccatttcat ctttaccttc ggtatagatc tcttttccgc caggtttgag taattccgca
    47401 agatcggcaa acacgtcaaa atcgttacgc gcttcaaatt gcggagcaac agcctgcttc
    47461 atcggcacaa tatgctggtt gctgtaatca ccggtcatcg tcaagtcatt gcgctcaaac
    47521 gatgtggtga tcggtaatac gatatctgcg tgtttggctg ctgcggtcca gtagcattca
    47581 gaaacgacga tcatctccgg tttctgccat gctttaatca gacgattagt atcctggtga
    47641 tgggtaaagt taccgccacc cgcccaccag atcattttga tattcggata cgtttgttct
    47701 ttaccgttat gctgatattt gccgccagga ttttccagcg catcgacgat acgggcaaca
    47761 ggaatcgccg tcattccgcc atcatcagcg gcctcgctgg catgtccggc aatcgccgca
    47821 gacatttccg gcagcacgcc gccaacacgc gtcgggttgc caccgttgga gtaatgataa
    47881 gagaaaccaa aaccgccgcc cggtgtacca atctgcccta acatcgccgc cagcgtcacc
    47941 agcatccagt gtttctgttc accatattgc tggcgctgaa ttccccagcc tgccatcagc
    48001 attgtgcgat tcgccgccat cagttcagcg agtttgacta tctgcgcttc cggtacgcca
    48061 gtaatttccg ccgcccagac cgcgctcttc ggcgtattat cgctcttacc tgtcagatac
    48121 tcttcaaact gcggataccc ggtagtgtat ttttcaagga acactttatc gtgtttgcct
    48181 tgtgtcatca gggtatgcgc aatccctaac atcagtgcca cgtcggtgcc catattcggc
    48241 gcgatccagg tggcattatc gtcaaagaat tcgatggttt cggagcggat aggatcaatg
    48301 gcaatcactg gtttgccaga ttttttcagc tgatggaagt attccagccc ttgctcatcg
    48361 gtactgctcc aggcaatttt taaggtattc agcgggttca ttccccacag caccacaacc
    48421 tggctgtttt ccagaatcag cggccaggag gtctgctgtt catacacctc tacagaaccg
    48481 accacatgcg gcatgatcac ctgtgctgcc ccggttgaat aatcgccgct atgcccggaa
    48541 taaccgcccg ccaggttcat ataacgttgc agtaaggttt gcgctttatg caacacgcca
    48601 gaagagcgcc agccgtaaga tccggcaaaa atggccgatg gtccgttagc tttacgaata
    48661 cgatcatgtt gctcatgaat cagttttaat gcctgttccc agctcacctg tacataggta
    48721 tcttcgccac gacctttcgc cggttgcagt ggattatcga gatagctttt tctcaccatc
    48781 ggatgctgaa tacgcgccgt ggtgtgtacc tgatccgccg ccgtagactg taaggaattc
    48841 ggtatggttt tcgccagcgc gcctgttgaa gaaacaatct tgccgtcctt cacttctacg
    48901 ttcatcgctc cccaacgtcc cgcggtgagg attttaccgc ccttctcttc tgcccatgcg
    48961 gggagcggtg ctgccgatgt caccaccagc gctccagcgg caataccgct gtgtttaata
    49021 aattcacgtc ttgttaatgt cataacttcc tccctgatca acgaggatca ctgtttctcg
    49081 gtaatatctt tggcgttgta ctggaaatac cgcgttaaaa tgtccagttc gttttcgctc
    49141 atgctggttc gtgcccccat tcctttggca atggacggcc acgcattgac ggtgtaatgg
    49201 tcggcggcaa taggggcatg acaaccagcg caataggtat cgtcaagttt ttcagcgtat
    49261 tgccatagcg gtttacggtc cgctaatgcg ggatcggtaa gcgcaccctg taaagacgcc
    49321 tgacgccatt gattgccgta ttcgtcagcc tgccattccc cgtttacagt gagcgccttg
    49381 ataccttctt cacttaatgt ggctagcgcc agccgttgac ctgccgccag gtagagcgtg
    49441 ttttcactgc cctgcatttg ataaccctgc aacagaacga tcggctgttt gccactggca
    49501 tcaacgacgg tgagatcggt tccaggattc acggtagcca gctcgcctat gtgagaagtt
    49561 ttgaaaggat aaatatgtgc gccattagta actgaagtag cggcctgact ttccagctca
    49621 tgcgccgcgt tgtcatccat ttttatttct ggcggaaaat gggcaatgcc tttatgacaa
    49681 tcgatacagg tttcgctgtc cttttgtgct ttgttatgca ttttctgcgc agattcactt
    49741 tgcgaggcaa tatccatggc atcaaaagaa tggcaactac ggcacgttgc agagtcagtg
    49801 gcttttaatt ctttccatac tgtttcggcc atttcctggc gatgagcttc gaacttatcg
    49861 tcactgtcta ttttgccgct aacaaattca tgataaatat ctttagatgc ctttaattta
    49921 gcaaataaat aatccatccc tgactttgga atatggcaat cggcacattc cgcacgtatc
    49981 cctttctggt tcgaaaagtg gacagttccc tgatattcct caaaaggttt actcatcgag
    50041 tggcaggaaa ggcaaaatgc tgtatccgac gttttatgta aggctttttg cgccagcaat
    50101 aacccgccgc caccaaccac aaccgctatc agcaaaaaca ataacccaat gcgttttttc
    50161 cctcgcatag attcctctaa ttaaattact ggacaccgaa atcattgtat aattaaatat
    50221 acaacgaatt aaccgcttgc ataattaggc acaac
//
LOCUS       DBANUU010000016        49544 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000016 DBANUU010000000
VERSION     DBANUU010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.016"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..840)
                     /locus_tag="ACIE68_000914"
     CDS             complement(<1..840)
                     /locus_tag="ACIE68_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFX5907267.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIKSSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFTQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTITDLLE
                     KPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEIDDLNAVNNYLLNNKKATDN
                     EIND"
     gene            complement(963..1415)
                     /gene="tabA"
                     /locus_tag="ACIE68_000915"
     CDS             complement(963..1415)
                     /gene="tabA"
                     /locus_tag="ACIE68_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFX5907268.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(1560..2153)
                     /gene="bdcR"
                     /locus_tag="ACIE68_000916"
     CDS             complement(1560..2153)
                     /gene="bdcR"
                     /locus_tag="ACIE68_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFX5907269.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPLARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            2224..2937
                     /gene="bdcA"
                     /locus_tag="ACIE68_000917"
     CDS             2224..2937
                     /gene="bdcA"
                     /locus_tag="ACIE68_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFX5907270.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     KRLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGEALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3068..3463
                     /gene="yjgH"
                     /locus_tag="ACIE68_000918"
                     /pseudo
     CDS             3068..3463
                     /gene="yjgH"
                     /locus_tag="ACIE68_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
     gene            3744..3878
                     /gene="pyrL"
                     /locus_tag="ACIE68_000919"
     CDS             3744..3878
                     /gene="pyrL"
                     /locus_tag="ACIE68_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFX5907271.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            3882..4817
                     /gene="pyrB"
                     /locus_tag="ACIE68_000920"
     CDS             3882..4817
                     /gene="pyrB"
                     /locus_tag="ACIE68_000920"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFX5907272.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            4830..5291
                     /gene="pyrI"
                     /locus_tag="ACIE68_000921"
     CDS             4830..5291
                     /gene="pyrI"
                     /locus_tag="ACIE68_000921"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFX5907273.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5364..5750
                     /gene="ridA"
                     /locus_tag="ACIE68_000922"
     CDS             5364..5750
                     /gene="ridA"
                     /locus_tag="ACIE68_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFX5907274.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNAAF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(5956..8652)
                     /gene="mgtA"
                     /locus_tag="ACIE68_000923"
     CDS             complement(5956..8652)
                     /gene="mgtA"
                     /locus_tag="ACIE68_000923"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFX5907275.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(8793..8846)
                     /gene="mgtL"
                     /locus_tag="ACIE68_000924"
     CDS             complement(8793..8846)
                     /gene="mgtL"
                     /locus_tag="ACIE68_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFX5907276.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            9031..9978
                     /gene="treR"
                     /locus_tag="ACIE68_000925"
     CDS             9031..9978
                     /gene="treR"
                     /locus_tag="ACIE68_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFX5907277.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            10097..11518
                     /gene="treB"
                     /locus_tag="ACIE68_000926"
     CDS             10097..11518
                     /gene="treB"
                     /locus_tag="ACIE68_000926"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFX5907278.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            11568..13223
                     /gene="treC"
                     /locus_tag="ACIE68_000927"
     CDS             11568..13223
                     /gene="treC"
                     /locus_tag="ACIE68_000927"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFX5907279.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWHDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NNGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALT
                     DESSVFYTYQKLIALRKQEAVLTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRG
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            13617..15755
                     /gene="nrdD"
                     /locus_tag="ACIE68_000928"
     CDS             13617..15755
                     /gene="nrdD"
                     /locus_tag="ACIE68_000928"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFX5907280.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            15913..16377
                     /gene="nrdG"
                     /locus_tag="ACIE68_000929"
     CDS             15913..16377
                     /gene="nrdG"
                     /locus_tag="ACIE68_000929"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFX5907281.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16422..16808)
                     /gene="cybC"
                     /locus_tag="ACIE68_000930"
     CDS             complement(16422..16808)
                     /gene="cybC"
                     /locus_tag="ACIE68_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFX5907282.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(16991..18343)
                     /gene="pmbA"
                     /locus_tag="ACIE68_000931"
     CDS             complement(16991..18343)
                     /gene="pmbA"
                     /locus_tag="ACIE68_000931"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFX5907283.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            18437..18988
                     /gene="yjgA"
                     /locus_tag="ACIE68_000932"
     CDS             18437..18988
                     /gene="yjgA"
                     /locus_tag="ACIE68_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFX5907284.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(19139..20512)
                     /gene="mpl"
                     /locus_tag="ACIE68_000933"
     CDS             complement(19139..20512)
                     /gene="mpl"
                     /locus_tag="ACIE68_000933"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFX5907285.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            20688..21686
                     /gene="fbp"
                     /locus_tag="ACIE68_000934"
     CDS             20688..21686
                     /gene="fbp"
                     /locus_tag="ACIE68_000934"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFX5907286.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(21719..22714)
                     /gene="yjfF"
                     /locus_tag="ACIE68_000935"
     CDS             complement(21719..22714)
                     /gene="yjfF"
                     /locus_tag="ACIE68_000935"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFX5907287.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(22701..23723)
                     /gene="ytfT"
                     /locus_tag="ACIE68_000936"
     CDS             complement(22701..23723)
                     /gene="ytfT"
                     /locus_tag="ACIE68_000936"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFX5907288.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMSQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(23737..25239)
                     /gene="ytfR"
                     /locus_tag="ACIE68_000937"
     CDS             complement(23737..25239)
                     /gene="ytfR"
                     /locus_tag="ACIE68_000937"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFX5907289.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGFLRRKEMEKRATELMTSYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(25549..26505)
                     /gene="ytfQ"
                     /locus_tag="ACIE68_000938"
     CDS             complement(25549..26505)
                     /gene="ytfQ"
                     /locus_tag="ACIE68_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFX5907290.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            26815..27345
                     /gene="ppa"
                     /locus_tag="ACIE68_000939"
     CDS             26815..27345
                     /gene="ppa"
                     /locus_tag="ACIE68_000939"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFX5907291.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(27425..27775)
                     /gene="chpB"
                     /locus_tag="ACIE68_000940"
     CDS             complement(27425..27775)
                     /gene="chpB"
                     /locus_tag="ACIE68_000940"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFX5907292.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(27769..28020)
                     /gene="chpS"
                     /locus_tag="ACIE68_000941"
     CDS             complement(27769..28020)
                     /gene="chpS"
                     /locus_tag="ACIE68_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFX5907293.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            complement(28233..28574)
                     /gene="ytfP"
                     /locus_tag="ACIE68_000942"
     CDS             complement(28233..28574)
                     /gene="ytfP"
                     /locus_tag="ACIE68_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFX5907294.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(28577..32356)
                     /gene="tamB"
                     /locus_tag="ACIE68_000943"
     CDS             complement(28577..32356)
                     /gene="tamB"
                     /locus_tag="ACIE68_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFX5907295.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TCGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(32353..34086)
                     /gene="tamA"
                     /locus_tag="ACIE68_000944"
     CDS             complement(32353..34086)
                     /gene="tamA"
                     /locus_tag="ACIE68_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFX5907296.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            34292..34930
                     /gene="msrA"
                     /locus_tag="ACIE68_000945"
     CDS             34292..34930
                     /gene="msrA"
                     /locus_tag="ACIE68_000945"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFX5907297.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            35253..36596
                     /gene="paeA"
                     /locus_tag="ACIE68_000946"
     CDS             35253..36596
                     /gene="paeA"
                     /locus_tag="ACIE68_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFX5907298.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(36658..36864)
                     /gene="ytfK"
                     /locus_tag="ACIE68_000947"
     CDS             complement(36658..36864)
                     /gene="ytfK"
                     /locus_tag="ACIE68_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFX5907299.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            37189..37746
                     /gene="ytfJ"
                     /locus_tag="ACIE68_000948"
     CDS             37189..37746
                     /gene="ytfJ"
                     /locus_tag="ACIE68_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFX5907300.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(37736..38476)
                     /gene="cysQ"
                     /locus_tag="ACIE68_000949"
     CDS             complement(37736..38476)
                     /gene="cysQ"
                     /locus_tag="ACIE68_000949"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFX5907301.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            38666..40609
                     /gene="cpdB"
                     /locus_tag="ACIE68_000950"
     CDS             38666..40609
                     /gene="cpdB"
                     /locus_tag="ACIE68_000950"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFX5907302.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(40738..41118)
                     /gene="ytfH"
                     /locus_tag="ACIE68_000951"
     CDS             complement(40738..41118)
                     /gene="ytfH"
                     /locus_tag="ACIE68_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFX5907303.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            41207..42067
                     /gene="qorB"
                     /locus_tag="ACIE68_000952"
     CDS             41207..42067
                     /gene="qorB"
                     /locus_tag="ACIE68_000952"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFX5907304.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSAGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            42175..43140
                     /gene="ytfF"
                     /locus_tag="ACIE68_000953"
     CDS             42175..43140
                     /gene="ytfF"
                     /locus_tag="ACIE68_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFX5907305.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            43248..43910
                     /gene="ytfE"
                     /locus_tag="ACIE68_000954"
     CDS             43248..43910
                     /gene="ytfE"
                     /locus_tag="ACIE68_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFX5907306.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLSRAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(44157..45224)
                     /locus_tag="ACIE68_000955"
     CDS             complement(44157..45224)
                     /locus_tag="ACIE68_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5907307.1"
                     /translation="MAILHPQECWLLERIMSPEYYRRRFEGWQEFVELCERQVAEWSK
                     TMPLDVRRRPLCEQIDVVWGGRVLPNIRSTLKSVQYDFIQLQQGDLRVLQSGGNISSD
                     MKGLIDYPSDWMSLAAQKQYDRLKWRGAHYNNLIRRTSGGYWYDGELTYYYEESLHGP
                     LALPMQLPLYELDSSVYLREDDSVTVAGLYLPDIPDASAQLLYRSEHIPEAWQGRVRT
                     KYVNEAGIQEYYWESGAWAKCNWKRIRRVANRFINVPPEGFFPQGMPEELYNWPQREA
                     QYVTDRQRIAAFSGEACPHSGEWSIFVEGRQATVTLEQGEQMPEWTDRKMEGEYKRGE
                     KFHVLWSLMNRHDGGSVWVEA"
     gene            complement(45329..45757)
                     /locus_tag="ACIE68_000956"
                     /pseudo
     CDS             complement(45329..45757)
                     /locus_tag="ACIE68_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246814.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            45884..45982
                     /locus_tag="ACIE68_000957"
                     /pseudo
     CDS             45884..45982
                     /locus_tag="ACIE68_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(46024..47427)
                     /gene="cycA"
                     /locus_tag="ACIE68_000958"
                     /gene_synonym="dagA"
     CDS             complement(46024..47427)
                     /gene="cycA"
                     /locus_tag="ACIE68_000958"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFX5907308.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVIVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRMAK"
     gene            complement(47736..48356)
                     /gene="fklB"
                     /locus_tag="ACIE68_000959"
     CDS             complement(47736..48356)
                     /gene="fklB"
                     /locus_tag="ACIE68_000959"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFX5907309.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            48575..49213
                     /gene="ytfB"
                     /locus_tag="ACIE68_000960"
     CDS             48575..49213
                     /gene="ytfB"
                     /locus_tag="ACIE68_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFX5907310.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(49197..>49544)
                     /locus_tag="ACIE68_000961"
     CDS             complement(49197..>49544)
                     /locus_tag="ACIE68_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFX5907311.1"
                     /translation="NPWFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGK
                     KHNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETIL
                     SWLLVDPSSTAHE"
BASE COUNT        12124 a        13267 c        12286 g        11867 t
ORIGIN      
        1 gtcattaatt tcattgtcag tagctttctt attattaaga agataattat ttactgcgtt
       61 aaggtcatct atttctttca gtatcccttt ttcaccaagc aagtatgaaa tcgattcatt
      121 acatacataa ggcattaata gcagatgttc tttccttatt gctaataatg cattattcaa
      181 tggtttttct aataaatccg taatcgtaga atcaactgaa tctttacaac tcacctgcga
      241 gttattcaag tcctgagaat tatttaactc ctgcggaata tttaatagac gagctatttc
      301 cttgttagct tcgcgttggt aacacgccag aatcttgtta gtaatcaggt gtaatgaact
      361 ctcatctttt ctttcaaagg acttcccttc ataacgatta gtcgaaccat caggttgtgt
      421 aaatataatg actgtatgtc cttcacgaat cccattatta aaagctgagg caagtgtaat
      481 ttctatttcc accttagata aatccgctcg ctccccttca ggattattca taacaaactt
      541 cattatctca ctaccatcat tatttttgat ataagatgag gacttaatat gagccatcat
      601 atttctaccc gcttcgttgg agcgtttgct aaaatccaca taacccaggg taaatatctt
      661 agccaaaaac tccaaaaatg ctctgggaga aagaatattg cttctgctat tctgccctcc
      721 ggcaattcgg gcaaaatcat tcattcccac ataccaggta tctaagtctt catttttttg
      781 tttacaattg tcggctgatg taatgttttg agaaaaattc caacctgata ttttgctcat
      841 gacggactcc ttgagttaat aagaacgtct aagcattaca aattctactc acaaaaaaat
      901 atcaaaaaac gctcttttca caaactcgcc gtaaacctgg agttatttac ggcgaaaagt
      961 catcacgcca tcagcatctt cactactgct ttgcgaacct gggctggcgc gcccactgcg
     1021 cacagcggtt tatgcacttc ccccggataa aacacaacaa aatcaccttc attaaggata
     1081 actgttttct catcaacgcc ttccggcaaa aatgcgatgt ctttatcagc taaccagtcg
     1141 gtatccggcg tgcctgcagg ttgcgtgctg aaggtcatgc cttcctgacc ttttaacaca
     1201 atctgaatgt cgagatagcg ggcatgatac tccgcacggc gagcttcgta cggctcggtc
     1261 atatcttccg agataagata aaacagacga ttgccttcga tatcgtgctt accctttggc
     1321 gtttctgccg taacgtgcgc tttgatatgc tcaatcgcct ggcgtaactc ctggggtagc
     1381 cacggctgaa gattatgaat atttccgatg atcatctttt ccactcctta attaaaacaa
     1441 tgtttcattt ctatcgttat acgaaaagat gatcaattcc gccagcctta acgcgtgaat
     1501 tattgtctta tcacaactta tcggcaggcg cgttcccgcc tttcggatca aggccagcat
     1561 cactccttga gaagagtttt tatcgcttcc cccgccagtg cagctgttgc acagagttgt
     1621 tcgattgagt gcccctcccg tgccttcgca gaaagccctg acatcacggt actcataaaa
     1681 tcagtcacac attgtgcgct ttgtggatgc cgcctggcga tatagtcata aatggtcgtt
     1741 tctgcggcgt gataatattg aacggcaata tcacgcgcta gtggatcatg actatgaata
     1801 ccttcaagaa ccatacagcc agcgcagccg ccgttttggc tatatctgcg cgccgcttct
     1861 tttaatacct caaccaggca ctcgcctact ggacgatcat cacgaagaat atcggcaagc
     1921 ggaatagctt ccgtaccgac gtattcattg agtacacggc taaataaccc agctttactg
     1981 ccaaaagccg cgtagaggct cggggggtta ataccaagat aatcagtaac ttcagcaaca
     2041 ctgacagcat cgaaaccttt ttggtgaaaa agcacttttg ccgcagagac tgcctgctca
     2101 ggagcgaaac gtctgggacg acctggaacg cgagattgtt ttttagtgac cattacaaaa
     2161 cttgttgaca gaaagttaaa acagttttgt aatgcatgtt acataataaa tcaaggagtc
     2221 cttatgggcg cttttacagg taagacagtt ctcatcctcg gtggcagtcg tggtatcggt
     2281 gccgctatcg tacgtcgttt cgtcaccgat ggggccaatg tacgattcac ctatgcgggg
     2341 tcgaaagatg ccgctaaacg cctggcacaa gagactggag cgacagcagt attcacagat
     2401 agtgctgaca gagacgctgt cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg
     2461 gtggtaaatg caggtattgg cgtctttggc gaggccctgg aattaaatgc cgacgatatt
     2521 gatcgccttt tcaaaatcaa tattcatgct ccttatcatg cctctgttga agccgcccgg
     2581 cagatgcccg aaggcgggcg catcttaatc atcggctccg tgaatggcga tcgtatgcct
     2641 gttgcaggca tggctgctta tgccgccagc aaatctgccc tacaaggcat ggcgcgcggg
     2701 ctggcccgtg atttcggacc gcgtgggatc accatcaacg tcgtccagcc agggccaatt
     2761 gataccgacg ctaatcccgc caacgggcca atgcgcgata tgttgcatag ttttatggct
     2821 atcaaaagac atgggcaacc ggaagaggtc gctggtatgg tcgcatggtt agcagggcca
     2881 gaagccagtt ttgttaccgg cgcgatgcat accattgatg gcgcgtttgg cgcataaccg
     2941 actacgctca attaagccca gccatttccc atgatgtctg ggttttgttt actcacgtcg
     3001 tccgctaaaa gcggctcctg gtaaatataa atcttctgtt catgtcgtaa atcttgagga
     3061 taaccaaatg gtagaaagaa ccgctgtttt ccctgctggc cgacattcac tgtatgcaga
     3121 acatcgttat tctgcggcta ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg
     3181 aagtcgagag gacggaacac cagaacccga ttttcagtaa caagtcagac tggcatttga
     3241 taatttgcat gcgaccctgg cagctgcggg atgcactttt gacgatatca ttgatgttac
     3301 gagctttcat accgatccag aaaaacaatt tgaagacatc atgacggtga aaaatgaaat
     3361 atttagcgcg ccaccttatc caacctggac ggcggtgggc gttacatggc tggcaggctt
     3421 tgattttgaa attaaagtga tagcgcgcat ccctgagctg taagcaatag tgttagccgt
     3481 tcgttttcac actccgccct ataagtcgga tgaatggaat aaaatgcata tctgattgcg
     3541 tgaaagtgaa aaagaaaaaa gcaggaaatg tctgcaatta ttgataccga acgacagttc
     3601 ccctccagaa tcacagtaaa taaaaatgca tatattttga cttataattc aaataaaccg
     3661 tttgcgctga caaaatattg catcaaatgc ttgcaccgct tctgacgatg agtataatgc
     3721 cggacaattt gccgggagga tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc
     3781 tgaaaaaaga cgctggcctg ccgtttttct tcccgttgat cacccattcc cagcccctca
     3841 atcgaggggc ttttttttgc ccaggcgtca ggagataaaa gatggctaat ccgctatatc
     3901 agaaacatat catttccata aacgacctta gtcgcgatga ccttaatctg gtgctggcga
     3961 cagcggcgaa actgaaagca aacccgcaac cagagctgtt gaagcacaaa gtcattgcca
     4021 gctgtttctt cgaagcctct acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc
     4081 ttggtgccag cgtggtgggc ttctccgaca gcgccaatac atcactgggt aaaaagggcg
     4141 aaacgctggc cgataccatt tcggttatca gcacttacgt cgatgcgata gtgatgcgtc
     4201 atccgcagga aggtgcggcg cgcctggcca ccgagttttc cggcaatgta ccggtactga
     4261 atgccggtga tggctccaac caacatccga cgcaaacctt gctggactta ttcaccattc
     4321 aggaaaccca ggggcgtctg gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg
     4381 gccgcaccgt tcactccctg actcaggcgt tagcgaagtt cgacggcaac cgtttttact
     4441 tcatcgcgcc ggacgcgctg gcaatgccgc aatacattct ggatatgctt gatgaaaaag
     4501 ggatcgcatg gagtctgcac agctctattg aagaagtgat ggcggaagtt gacatcctgt
     4561 acatgacccg cgtgcaaaaa gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc
     4621 agtttgttct tcgcgccagc gatctccaca acgccaaagc caatatgaaa gtacttcatc
     4681 cgctgccgcg tgttgatgag attgcgacgg atgttgataa aacgccacac gcctggtact
     4741 tccagcaggc aggcaacggg attttcgctc gccaggcgtt actggcactg gttctgaatc
     4801 gcgatctggt actgtaaggg gaaatagaga tgacacacga taataaattg caggttgaag
     4861 ctattaaacg cggcacggta attgaccata tccccgccca gatcggtttt aagctgttga
     4921 gtctgttcaa gctgaccgaa acggatcagc gcatcaccat cggtctgaac ctgccttctg
     4981 gcgagatggg ccgcaaagat ctgatcaaaa tcgaaaatac ctttttgagt gaagaccagg
     5041 tagatcaact ggcattgtat gcgccgcaag ccacggttaa ccgtatcgac aactatgaag
     5101 tggtgggtaa atcgcgccca agtctgccgg agcgcatcga caatgtgctg gtctgcccga
     5161 acagcaactg tatcagccat gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg
     5221 ccaatgacat cgcgctcaaa tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc
     5281 tggccaatta attgcggttg gtaataaaag tctggctccc tataatgagc cagacttttt
     5341 accgctgtaa taaaggagaa atcatgagca aaactatcgc gacggaaaat gcaccggcag
     5401 ctatcggccc ttacgtacag ggcgttgatc tgggcaatat gatcatcacc tccggtcaga
     5461 tcccggtaaa tccgaaaacg ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt
     5521 cgctggataa cgtaaaagcg atcgtcgaag ccgctggtct gaaagtgggc gacatcgtta
     5581 aaactaccgt gtttgtaaaa gatctgaacg acttcgcaac cgtaaacgcc acttacgaag
     5641 ccttcttcac cgaacacaac gccgccttcc cggcacgttc ttgcgttgaa gttgcccgtc
     5701 tgccgaaaga cgtgaaaatt gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga
     5761 aatccgggct atcatgcccg gattaagtct gatgacaaac acaaaatcgc ctgatgcgct
     5821 acgcttatca ggcctacgtg attcctgcaa tttattgaat ttgttggccg gataaggcat
     5881 ttacgccgca tccggcatga acaaaactca ctttgtctgc aattcgaatc caggccacca
     5941 tgcccagttg attctttatt gccagccgta acgacggcta taaaatcctt tcaccaactg
     6001 ggttaatgtc atataccctg ccagaatcgc aaccagccac gggaaatagc ttaacggcag
     6061 cgcctgcagt tgcagataac tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat
     6121 cacgatcacg gtcatgatca ttaacggcca cgatgcacag ctctgaataa acggcacacg
     6181 gcgggtgcgg atcatatgca caatcagcgt ttgcgacagt aagcccacca caaaccatcc
     6241 cgactggaac agcgtttgcg tttccggcgt gttggcatgg aatacccacc acatcaggca
     6301 aaacgtcaga atatcgaaga tcgagctgat cggtccgaag aagatcataa agcgccccag
     6361 atccgccgga ttccaacgct gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg
     6421 gatcgccacc tgtgacacat catacagcag gttctgaata agcaagtgta acggcaacat
     6481 cggcaggaag ggcaagaaag cactcgccac cagcacgctg aacacattac cgaagttgga
     6541 gctcgccgtc attttgatgt atttcagcat gttggcgaaa gtgcgacgtc cttcaataac
     6601 cccctcttcc agcaccatca ggcttttttc cagcaggatg atatcagccg cttcacgggc
     6661 aatatctacc gcgccgtcca cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc
     6721 attaatacca tcgcccataa agccaaccac atgcccttcg cgcttcagta aggtcacaat
     6781 gcgttcttta tgcatcggcg tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc
     6841 cagttcgtcg tcagatagcg tttcaatatc actaccaatg accacctctc ccgcgtccag
     6901 ccccacttca tggcacactt tcgctgcaac taactcactg tcgccagtaa ggatttttac
     6961 ggtaattcca ctcgctttta atgccttcag tgccggagca gttgtctctt ttggcggatc
     7021 aaggaaagca atatatcctt cgaggatcag gtcggattca tccgcccgct ggtaatctcc
     7081 ttcacgcgct ggcaggtatt tcgtcgccac cgcaaccacg cgcagcccct gacgattcag
     7141 cgtatcagta acccgcttaa tcttacgcag catgatgtca tcgagcggca caatctcgcc
     7201 attgtgacgc acctgcgaac acacattgag gatttcctgc aatgcacctt tgcaaaccag
     7261 ctgatggtgc tcggtatttt ctgccactac caccgacatc cggcgacgct cgaaatcaaa
     7321 cggaatctca tcgatttttt gccaacgact ggccagcgag cgcgctgact cttcatccgt
     7381 accttcgagc accgctgtat cgagcaggtt tttaagtccg gtctgataat gactgttcaa
     7441 ccacgcgcta tgcagcacgc gttcgctggt tttaccggag atatcggtat gattctccag
     7501 cacaattttg tcctgtgtca gggtgccagt tttatcagtg cacagaatat ccattgcgcc
     7561 aaagttctga atagcatcca gatgtttgac gatcactttc tgtttcgaca gctttactgc
     7621 gccgcgcgcc agcgtcgagg taacaatcat cggcaacatt tccggcgtta agcctaccgc
     7681 gaccgaaagc gcaaacagtg ccgcttccca ccagtcgcct ttggtgtaac cgttgataag
     7741 caacaccacc ggagccatca ccagcataaa gcgaatcagc agcatactga cgcggctgat
     7801 cccctgctga aaggcattcg gctcgctttc ctgctcacta acacgccccg ccagttgacc
     7861 aaaccaggta ttggctcctg tagcaatcac cattgcttgt gccgtgccgc tcaccacggt
     7921 ggtgcccata aaacacaggg tgtcacactc cagcggattg ctgtgctccg gctggcgagt
     7981 ggtagcagct ttttctacgg gcagagactc accggttaac gacgcctgag cgacgaacag
     8041 atcccgcgcc tgcaagatac gtaaatctgc cgggatcata tcccccgccg ccagttttat
     8101 aatatcgccg ggcaccagct ggtcgatcgg gatctccagc cagccatttt cgcctttgtc
     8161 gttaattacg cgcagcaccg tcgcggtatt gctgaccatc gctttcaggg catctgccgc
     8221 tttagtggaa cgtgcttcct gaataaagtt cagcaacgta gaaatagcga ccattagcgc
     8281 gataacgccc gcggcaaata aatcttccgt ggcgtaagaa atagcgccga gaatggtgag
     8341 taaaatatta aaggggttgc gatagcagac ccataaatgt acccaccacg gcgacggttg
     8401 ttgtgcgggt aatttatttt caccatgttg ttcgcgggca gattccactt ccgcctgatt
     8461 taacccttcc ggatgggtgt cgaacgtttt ccacaattct tcttcgggca tcaccgccat
     8521 tttcaggcaa tgcgcactta aggacggcgg gaccaccgta ttcactgtct gctgcgcgcc
     8581 aggcaatgga tcacgatgaa ccagacggga aggtaaatgg cgaatgagcc gggtaaagat
     8641 ttctttaaac ataaggagtc cctccgcact gtctgaaaca gtacagaaat gtcttcaggc
     8701 gtggcgaagc cttacctgaa ggtaataaag ctgagcaggg acgttaaaac gtcacggcct
     8761 tacgcatccg gtaacgccgt gaaaagacgg gcttaccgga aaagttttaa tctccgtcga
     8821 gggagaggcg tgggatcagg ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat
     8881 aataaggaga atccaataat attcgtagca tctatggcga tataatacct gctggcaact
     8941 aaaccaacga taaaccagac tttacccatt gctgaatgca cgggtaaagt taggctcaaa
     9001 taattaaaca acacgttaca ggacaacagg atgcaaaatc ggctgaccat caaagacatc
     9061 gcacgcttaa gcggcgtggg gaaatctaca gtttcccggg tgctgaataa cgaaagcggc
     9121 gtgagccagc gcacccgcga gcgtgttgaa gcagtgatga atcagcatgg attttcccct
     9181 tcccgctctg cgcgcgctat gcgtgggcaa agcgataaag tggtcgccat cattgttacc
     9241 cgcctggatt cgttgtcaga aaatctcgcc gttcaaacca tgctgccagc gttctatgaa
     9301 caaggttacg acccaatcat gatggaaagt cagttttccc cgcaattagt tgccgaacat
     9361 ttgggggtgc tgaaacggcg taatatcgac ggcgtagtgc tgttcggttt tactggcata
     9421 acagaagaaa tgttagccca ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa
     9481 ggttttgctt cggtctgtta tgacgacgaa ggggcaatca agatcctgat gcaacggctg
     9541 tatgaccagg ggcatcgtaa tatcagttat ctcggcgtgc cgcatagtga cgtgacgacc
     9601 gggaagcgac gtcacgaagc ctacctggcg ttctgcaaag cgcataaact gcatcccgtt
     9661 gccgctctgc caggccttgc tatgaagcaa ggctatgaga acgtagcaaa agtgattacg
     9721 cctgaaacta ccgccttact gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac
     9781 ctgcaagagc aacgcatcga caccttgcaa ctggcgagcg tcggtaatac gccattaatg
     9841 aaattccttc atccggagat cgtcaccgta gatcctggtt acgccgaagc tggacgccag
     9901 gcggcctgcc aattgatcgc acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc
     9961 cccgccaccc tgtcctgatc gtttcctgaa cgataaattg tgatcttcgc tgcgtttcgg
    10021 gaacgttccc gtttttaaat ttttccgcgc aatatattct gcagccaacc aaaaatgtca
    10081 tctgccatgg ggctttatga tgagcaaaat aaaccaaacg gatatcgatc ggttgattga
    10141 actggtcggc gggcgcggca atattgcgac ggtgagccac tgtattactc gcctgcgctt
    10201 tgtcctcaac caaccggcca atgccagacc gaaagaaatt gagcaactcc ccatggtgaa
    10261 aggctgtttc accaatgccg ggcaatttca ggtggtgatt ggcaccaatg tgggtgatta
    10321 ctatcaagca ctaatagcgt caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa
    10381 agccgcccgg cagaatatga aatggcatga gcagttgatc tctcatttcg cggagatctt
    10441 cttcccgttg ctgcccgcgt tgatcagcgg cggtttgatc ctcggttttc gcaatgtgat
    10501 cggcgatttg cccatgagca acggtcagac gctggcgcaa atgtaccctt ccctgcaaac
    10561 gatctacgat tttctgtggt tgatcggtga agcgatcttc ttctacctgc cggtcggtat
    10621 ttgctggtca gcggtgaaaa aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt
    10681 gacactggtt tctccacagc tgatgaacgc ttatctgctc gggcagcagc tgccggaagt
    10741 gtgggacttt ggcatgttca gcatcgccaa agtaggctat caggcgcagg tgatcccggc
    10801 actgttagcc ggactggcgc tgggcgttat tgaaactcgc cttaaacgca tcgtgccgga
    10861 ttacctctat ctggtggtgg tacccgtctg ttcgctgatc ctcgcggtgt tcctcgccca
    10921 tgcgctgatt ggtccgtttg gtcgcatgat tggcgatggc gttgcctttg cggtacgtca
    10981 cctgatgacc ggcagctttg ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc
    11041 gctggtgatc accggcgtac accagaccac ccttgctatt gatttgcaga tgattcaaag
    11101 catgggtggc acgccagtgt ggccgctgat tgcgctgtcg aatatcgctc agggctccgc
    11161 cgtaataggc attatcattt ccagccgcaa gcacaatgag cgcgagatct ccgtgcctgc
    11221 cgctatctcc gcctggcttg gggtcactga gcctgcaatg tacggcatca acctgaaata
    11281 tcgcttcccg atgctgtgcg cgatgattgg ttctggtctg gcaggattgc tatgcggcct
    11341 gaacggcgtt atggcgaatg gtatcggcgt aggcggcctg ccgggaattc tctcgattca
    11401 accgagctac tggcaggtgt ttgcgctggc aatggctatc gccatcatca tcccgattgt
    11461 actcacctcg tttatctatc agcggaaata ccgcctgggc acgctggaca ttgtttaatt
    11521 ttcttcgggg cgcaattgcg ccccctcgca tttgcaggaa taacgtaatg actaatcttc
    11581 cccactggtg gcaaaacggc gttatctacc agatttatcc aaagagtttt caggacacca
    11641 cgggtagcgg caccggcgat ttacgtggcg ttatccaacg cctggactat ctgcataaac
    11701 tgggcgttga tgccatctgg ctaaccccct tttatgtctc tccccaggtc gataacggtt
    11761 acgatgtagc gaactatacg gcgattgatc ccacctacgg cacgctggac gattttgacg
    11821 aactggtgac gcaggcaaaa tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc
    11881 atacctctac ccaacatgcc tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc
    11941 agttttatat ctggcacgat ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat
    12001 ttggcggtag tgcgtggcgc tggcatgcgg aaagcgaaca gtactatttg catctctttg
    12061 caccagaaca ggcggatctc aactgggaga atccagcggt acgcgcagag ctgaaaaaag
    12121 tctgtgaatt ctgggccgat cgtggggtcg acgggttgcg cctggatgtg gtgaatctga
    12181 tctccaaaga cccgcgtttc cctgaagacc tggatggcga cgggcgtcgc ttctacaccg
    12241 acgggccacg agcacacgag tttttgcacg agatgaaccg cgatgtgttt acgccacgcg
    12301 ggttaatgac cgtaggtgaa atgtcctcca ccagccttga gcattgccag cgatacgcgg
    12361 cactgacagg cagtgaattg tcgatgacct ttaattttca tcacctgaag gtcgattatc
    12421 ccggtggtga aaaatggacg ctggctaaac ctgactttgt ggcgttgaaa acattgttcc
    12481 gccactggca acaaggaatg cacaacgtgg catggaatgc cttgttctgg tgtaaccacg
    12541 atcagccgcg cattgtttct cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa
    12601 aaatgctggc gatggtgctg catggcatgc agggaacgcc gtatatctac cagggcgaag
    12661 agattggcat gaccaacccg catttcacgc gcattactga ctatcgcgac gtggagagcc
    12721 tcaatatgtt tgccgagctg cgcaacaatg ggcgtgatgc cgacgagtta ttggcaatcc
    12781 tcgccagtaa atcccgtgac aacagtcgca cgcccatgca atggagcaac ggcgataatg
    12841 ccgggtttac ggctggcgaa ccgtggattg gcctgggcga taactatcaa caaatcaacg
    12901 tagaagccgc gctgaccgat gagtcctcgg tgttttacac ctaccaaaag ttaatcgcac
    12961 tgcgtaagca agaagccgtt ctgacatggg gcaattacca ggatctgctg ccaaacagcc
    13021 ctgtattgtg gtgctatcgc cgtgaatgga aggggcaaac cttgctggtc attgccaacc
    13081 ttagccgtgg gatccaaccc tggcagccag ggcaaatgcg cggcaactgg cagcttgtga
    13141 tgcataacta cgaagaagcc tcaccacaac cctgtgccat gaatttacgg ccttttgagg
    13201 ctgtctggtg gttacagaag taaatcttcc ctaagccccg gtaatgccgg tcattccggg
    13261 gttttgtctg tcagcatgtt gtttttgttg atttttcaac cagcaaattc attaaaaaat
    13321 ttacataacg ctgtagcgcc cgtcatccgt acgctctgct ttttactttg agctacatca
    13381 aaaaaagttc aaacatcctt gatgcaaagc actatatata gactttaaaa tgcgtcccaa
    13441 cccaatatgt tgtattaatc gactataatt gctactacag ctccccacga aaaaggtgcg
    13501 gcgttgtgga taagcggatg gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg
    13561 gaaaacgcct ttcccaattt ctgtggataa cctgttctta aaaatatgga gcgatcatga
    13621 caccgcatgt gatgaaacga gacggctgca aagtgccgtt taaatcagag cgcatcaaag
    13681 aagcgattct gcgtgcagct aaagcagcgg aagtcgatga tgccgattat tgcgccactg
    13741 ttgctgcggt tgtcagcgag cagatgcagg gccgcaacca ggtggatatc aacgagatcc
    13801 agaccgcagt tgaaaaccag ctgatgtcgg gtccatacaa acaactggct cgtgcttaca
    13861 tcgagtaccg tcacgatcgc gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga
    13921 tccgtggtct ggtcgagcag accaacgcct cgttactcaa cgaaaacgcc aacaaagaca
    13981 gcaaggtgat tccaacccag cgcgacctgc tggccgggat cgtggctaaa cactatgcac
    14041 gccagcacct gctgccgcgt gacgtggtgc aggcacatga gcgtggcgat attcactatc
    14101 acgatctcga ttactcgccg ttcttcccga tgttcaactg catgttgatt gacctgaaag
    14161 gcatgctgac ccagggcttt aaaatgggga acgccgagat tgaaccgccg aagtcgatct
    14221 ctacggcaac cgctgtaact gcgcagatta ttgctcaggt cgccagccat atttatggcg
    14281 gcaccaccat taaccgtatc gatgaagtgc tggcaccgtt tgtcaccgcc agctacaaca
    14341 aacatcgcaa gaccgcagaa gagtggaaca tcccggacgc cgaaggctac gctaactctc
    14401 gtaccattaa agagtgctac gatgccttcc agtcgctgga gtacgaagta aacaccctgc
    14461 acaccgccaa cggtcagacg ccgtttgtaa cctttggttt tggtctgggc accagctggg
    14521 aatcgcgcct gattcaggaa tctatcctgc gtaaccgtat cgcaggcctt ggtaaaaacc
    14581 gtaaaactgc ggtgttcccg aaactggtgt ttgcgattcg cgatggcctg aaccataaaa
    14641 aaggcgatcc gaactacgac atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt
    14701 atccggatat cctgaactac gatcaggtag tgaaagtcac cggttcgttt aaaaccccga
    14761 tgggctgccg cagcttcctc ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg
    14821 gtcgtaacaa cctcggcgtg atcagcctga acctgccgcg tattgctctg gaagcaaaag
    14881 gcgatgaagc caccttctgg aagctgctgg atgaacgtct ggtgctggca cgtaaggcac
    14941 tgatgacccg tatcgctcgt ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata
    15001 tggaaggtgc ttgtggcgta cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa
    15061 acggtcgtgc gtctatttcg ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt
    15121 tcggcggcga gcatgtttac gacaacgagc agcttcgcgc gaaaggtatc gccattgttg
    15181 aacgtctgcg ccaggcagtg gatcagtgga aagaagaaac gggttatggt ttcagtctct
    15241 acagcacgcc gagtgaaaac ctgtgcgacc gcttctgccg tctcgatact gctgagtttg
    15301 gcgtggtgcc gggcgtgacc gataaaggtt actacaccaa cagtttccac ctcgatgtgg
    15361 agaagaaggt gaacccgtac gacaagatcg actttgaagc gccttacccg ccgctagcga
    15421 acggtggttt catttgctac ggcgagtatc caaacattca gcacaacctg aaggcgctgg
    15481 aagatgtctg ggattacagc tatcagcatg taccgtatta cggcaccaat acaccgattg
    15541 atgagtgcta cgagtgtggc tttaccggtg agttcgagtg caccagcaaa ggcttcactt
    15601 gcccgaaatg tggtaaccat gacgcctccc gtgtgtcggt gactcgccgc gtgtgcggat
    15661 atttaggtag cccggatgca cgtccgttta acgctggtaa gcaggaagaa gttaagcgcc
    15721 gcgttaaaca tttggggaat gggcagatag gttaatcttc actctgcgct aagcaaatgc
    15781 cggatgcggc gtaaacgcct tatccggcct acatttggca acggctccag gcctgataag
    15841 acgcgacagc gtcgcatcag gcattgatta ccggatgcag cctacacgcg gactgataag
    15901 ccaggcgaaa taatgaatta tcatcagtac tatcctgtcg acatcgtcaa cggccccggc
    15961 actcgctgca ccctgtttgt ctccgggtgt gttcatgaat gccccggttg ctataacaaa
    16021 agcacatggc gggtaaattc tggtcagcca tttaccaaag caatggaaga caagatcatt
    16081 aaagatctga atgacactcg tatcaaacgc caggggattt ccctctccgg cggcgatccg
    16141 ctgcatccgc aaaacgtgcc ggatattctg aaactggtaa aacgcatccg cgccgagtgt
    16201 ccgggtaaag acatctgggt gtggacaggc tataaactcg acgaactcaa cgctgcgcaa
    16261 atgcaggttg ttgatttgat taacgtactg gtcgacggca aatttgtgca ggatttaaaa
    16321 gacccttccc tgatctggcg cggcagcagc aatcaggtgg tgcatcattt gcgatgataa
    16381 gttccctgtt acctgagtgc aggcaacagg gaaatgagga attaacgata cttctggtga
    16441 taggcgttgc gggtcgtttt cagttgctct gcagcagcct gcgcttcttt tactttacct
    16501 tcatttgcca gcttcagcgc gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg
    16561 aaatctttca tttccgggct gtccggtgat ttatcttcga gcttcggcgg cgttgctttt
    16621 tgcgcatcca gcgctgcggc gcgcatcttc gttaacgcgt ctttgacttg cgccgcgtta
    16681 tccgcttttt cgatcacttt taaattgtcg ttgagggttt ccatattgtc ttcaagatca
    16741 gcggcaaacg acgcagaact gaataccaac gaggagactg caagaatagc taacaggctt
    16801 ttacgcattg ctcacttcct tttttattta cacttcatcc ttcacactgc ctcatcgacg
    16861 gctacgctca ttcgcccggg tcacgtactg cgagtacgct ccccggccct cattcacttt
    16921 ccgtctcgat gcagtgcgaa tgattttgtg taatttttta gtttatagcg cggcaggtcg
    16981 cgccagtttt ttactgcccg gcgattttca tctccggcaa cagcacagaa ccacactgta
    17041 tattactgcg tgtttcaata tcgttaccga cggtgacaat attgcgccac atatctttta
    17101 aattacctgc gatggtgatt tcactaacgg gatactgaat ttcgccattc tctacccaga
    17161 agcccgctgc accacgggaa taatcaccgg taatggcact cacgccctgg cccatcagct
    17221 ccgtcaccac cagcccggtg cccatctctt tgagcatttg ttcgaagctt agcccttgtc
    17281 cggcaatccg ccagttgtga ataccgcccg catgtccggt gcttttcagc cccagtttcc
    17341 gcgccgagta gctggtcagc agccactgag tcaggatgcc atctttaata atatcgcgac
    17401 gctcggtgcg cacaccttcg ctgtcgaatg gcgtcgacgc cagccctttc agcagatgcg
    17461 gatgctcttc aatggtcagc cagtccggca gaatttgttt acccagcgaa tccagcagaa
    17521 aggtagattt acggtaaacc gagccacccg ctatcgcccc caccagatgg ccaaaaagcc
    17581 cggttgccac ttcattggca aaaatgactg gcgctttcat ggtggagagt ttacgcggtg
    17641 acaggcgcga taaagtacgg cgagcacagt cggccccaac ccactctggc gtttgcagat
    17701 cgctcatcgc acgaccaatg gtgtaggcgt aatcacgctc catatcgcca ttttcttcgg
    17761 caattacaca gctggagagt gaatgacgcg tagagcagta accctgcaac atgccgtggc
    17821 tgttgccaaa aactttgaca ccgtagtggc tgttaaaact gccgccttcg gtattggtaa
    17881 tgcgtttgtc cgcctgcaat gccgcctgtt ctgcgcgggc cgccagttca atggcttcat
    17941 ccggggaaac ttccgcaggg tggaacaagt cgagatctgg tgcgtcaaag gccagcagct
    18001 ctttgtctgc cacgccggca cagggatctg gcgaggtata acgggcaata tccagcgccg
    18061 cctgtacggt gcgggcaatg gcctgcgggc ttaaatcggt ggatgatgcg ctacctttgc
    18121 ggttctggtg ataaacagtg atccccagcg cgccatcgct attgaattca acattctcca
    18181 cttcaccata acgcgtgctt acgctaatgc cggtggtctt gctgacggca acttccgcac
    18241 cgtccgattt gcctgaggcc aactccagcg cagtggaaac tgcttcttcc agaatcttgc
    18301 gctgcgcttc aacttgagag attactttca ttgcaagtgc cataatgtag agagtttctc
    18361 tgaagtctaa cagagaaccg tttttcagtg cgcatcttaa ctggtaacat tagcctcttt
    18421 ttttaaggag cctgagatga ctaagcagcc cgaagactgg ctcgacgacg ttcccggtga
    18481 tgacatcgaa gacgaagacg atgaaattat ctgggtcagt aaaagtgaaa ttaaacgtga
    18541 tgccgaggag ctaaaacgcc ttggcgcgga aattgttgat ctggggaaaa acgcgctgga
    18601 taagatcccg ttagatgcgg atctgcgcgc ggctattgag ctcgcccagc gtattaagat
    18661 ggaaggtcgc cgccgccagt tgcagctcat cggtaagatg ctgcgccagc gcgacgtaga
    18721 gcctattcgt caggcgctgg ataagctgaa aaaccgtcac aaccagcagg tggtgctgtt
    18781 ccataaactg gaaaacctgc gcgatcgtct gatcgatcaa ggtgatgacg ccatcgctga
    18841 agtattgaat ctgtggcccg atgccgatcg tcagcagctg cgtactctga tccgtaacgc
    18901 gaagaaagag aaagaaggga ataagccgcc gaaatccgca cgccagattt tccagtatct
    18961 gcgcgaactg gcggagaatg aagggtaatt ttcgttgttg atgtgagtat ggtttgccgg
    19021 atgcggtgca cctgtaggcc ggataagacg catcaagcgt cgcatcaggc actatgctca
    19081 aatgccggat gcggcgtaaa cgccttatcc ggcaatgcta tatcaggctg aagccgaatt
    19141 actgcgcagc ttccgccttc ttcgccagac catccagcag tttctgatgg atcccaccaa
    19201 aaccgccgtt gctcatcacc agaatatggt cgccaggctg agcggttttc accaccatat
    19261 ctgccagcgt atccacatcg ccactccagt gtgcaggctg aacgcaggct tctgccactt
    19321 ctgccacctg ccacggaata tgcgccggtt gcagcaggaa gacttcatcg gcacgaccta
    19381 atgaaggtgc cagatcgtct ttgcagatcc ccattttcat ggtattcgag cgcggctcca
    19441 gcacagcaat aatgcgcgcc gtaccaccaa ctttgccacg cagcgccgcc agcgttgcca
    19501 gaatcgccgt cgggtgatgg gcaaaatcgt catataccgt gacgccattc gcttcaccac
    19561 gcaactccag acggcgacga gcattaataa acgaacccag cgcgttagcg gcatctgccg
    19621 gcgctacacc aacatggcga gccgctgcaa tcgccatcag gccattgtgc atattatgtt
    19681 cgcctaccag cgaccatttc acttcgccca ctttttcgcc atccagcaaa acttcccatt
    19741 cggaagcatc ggtggtcagc tttttcgcct gccagtgacc ctgctcaccc accagctcct
    19801 gctcgctcca gcagcccatc gccatggtct gtttcaggtt gatgtcattt tccggccaga
    19861 taatacggcc ctgccccgga acgatacgca ccagatggtg gaactgtttc tggatcgctt
    19921 tcaggtcgtc aaagatatcg gcgtgatcga actcaaggtt gttgaggatc agcgtacgcg
    19981 ggcagtaatg gacaaattta gagcgtttgt cgaagaaggc gcagtcatac tcatccgctt
    20041 cgataacaaa gaagtcgctt tcgcccagac gcgccgaaac ctcaaagttc cccggcacac
    20101 cgccgatcac aaatcccggt ttgtaaccgc actgttccag aatccaggtc gccattcccg
    20161 cggtggtagt tttgccgtgt gtaccggcaa cggccagcac ccagcggtcg cgcagcacaa
    20221 aatcgtgcag ccactgtgga cctgacatat aagggatgtt tttttccagt accgcttcca
    20281 cacacggatt tccacgggtc atggcgttgc caataatcac cagatccggc tgcggatcga
    20341 gctggctggc atcgtaaccc tgaatcagct caatgccttg cttctcaagt aaggtgctca
    20401 tcggcggata cacattggcg tccgaacccg ttacttcatg gcctaactgg cgcgccagca
    20461 tcgccagacc gcccataaat gtgccacaaa ttcctaaaat atgaatgcgc atacgtcact
    20521 atcctttttt aatctgccgc tcattttacg catatgtcgg gcaagtgaga aacgcatttc
    20581 aggataatcc gcaatttgct ggcgcgattc acctgagcgc aacattgaga ttatttgtta
    20641 agattgttgc ggtcgcttta ctccataaac attgcaggga aagttttatg aaaacgttag
    20701 gtgaatttat tgtcgaaaag cagcacgagt tttctcatgc taccggtgag ctcactgctt
    20761 tgctgtcggc aataaaactg ggcgccaaga ttatccatcg cgatatcaac aaagcaggac
    20821 tggttgatat cctgggtgcc agcggtgctg agaacgtgca gggcgaggtt cagcagaaac
    20881 tcgacttgtt cgctaatgaa aaactgaaag ccgcactgaa agcacgcgat atcgttgcgg
    20941 gcattgcctc tgaagaagaa gatgagattg tcgtctttga aggctgtgaa cacgcaaaat
    21001 acgtggtgct gatggacccc ctggatggct cgtccaacat cgatgttaac gtctctgtcg
    21061 gtaccatttt ctccatctac cgccgcgtta cgcctgttgg cacgccggta acggaagaag
    21121 atttcctcca gcctggtaac aaacaggttg cggcaggtta cgtggtatac ggctcctcta
    21181 ccatgctggt ttacaccacc ggatgcggtg ttcacgcctt tacttacgat ccttcgctgg
    21241 gcgttttctg cctgtgccag gaacggatgc gcttcccgga gaaaggcaaa acctactcca
    21301 tcaacgaagg aaactacatt aagtttccga acggggtgaa gaagtacatt aaattctgcc
    21361 aggaagaaga taaatccacc aaccgcccgt atacctcacg ttatatcggt tcactggtcg
    21421 cggatttcca ccgtaacctg ctgaaaggcg gtatttatct ctacccaagc accgccagcc
    21481 acccggacgg caaactgcgt ttgctgtatg agtgcaaccc gatggcattc ctggcggaac
    21541 aagcgggcgg taaagcgagc gatggcaaag agcgtattct ggatatcatc ccggaaactc
    21601 tgcaccagcg ccgttcgttc tttgtcggca acgaccatat ggttgaagat gtcgaacgct
    21661 ttatccgtga attcccggac gcgtaatctc ctgcgcctct ccacacggag aggcgttttt
    21721 atcgctgcgc aatattgact cttgtcactg gcgaactctg acgattctcc cacagcaccg
    21781 ttaatccacg ctgtaaggca ataaaaatga acagcaaaat gccgatggcg attttcgtcc
    21841 accaggaact cagcgtaccg tcaaagttga tataagtctg aattagcccc tgaatcgcca
    21901 cgccaaaaag cgtccctaat accgttccaa cgccaccgct caaaagcgta ccgccaatta
    21961 ccactgaggc gatagcatcc agttccacac ctacgcccgc cagcgcatat ccggcctggg
    22021 tataaatcga gaagacaatc cccgccagcg ttgccagtcc ggtggagagc atataaatgc
    22081 gaatagtggt gctgcgggta gaaatcccca tcaggtttgc cgacgttgcg ttgccgccaa
    22141 tggcgtatac ctgattgcca aaacgggtac gatgcgcaag gaaaataccg ataaccacca
    22201 ccgccagcat caacagcccc atcgcactaa ggcgaccgcc gccggggatt ttccacgcaa
    22261 ggcttgagag cgtgtcataa atgggatggt ttatcggaat cgactcttcc gaaacgagat
    22321 agctgacgcc gcgcaaaaag aacatccccg caagcgtaat gataaatgcc gggatcttca
    22381 gggcgtcgat cagaagcccc ataaatgcgc cgaaggcaca gcccatcacc agcactagcg
    22441 gaaacgccag cagcggcgag aggccgaaat cgccaaccac ttttgccaga aacacgccag
    22501 taaaggcgat caccgaaccg acggagagat cgataccgcc agagaggatc acaaaggtca
    22561 tgccaacggc gattatccca agaaaggcgt tatcggtcag gatattgcag atcactcttg
    22621 tggaagcaaa accgggaaac tgcgtcaggc agtagaaata gcccagcaca aagacgccga
    22681 tggtgatcat cagtggcaaa ttacgtttta tcatggctac gtactccttt aatcagactg
    22741 ataaagcgtt gcgactgaac aatcagcaca caaagcacca ccaccgcttt tacgacctgg
    22801 ttcatctccg gcggaaagcc agaaagtaaa attccggtgt tcatcccctg aataatcagc
    22861 gcccccacga ctgagagcag caggttaaag cgcccgccca tcagcgatcc gccgccaatc
    22921 accaccgcaa gaatggcgtc cagctccagc cataacccgg cgttattggc atcggcaccg
    22981 cgaatatccg ccgccacgat aatgcccgca atcgccgcac acagcccgct caacacgtag
    23041 gtgagcatga cgatgatccg cgtgtttacc ccggcatttt ttgccgcccg aatgttgata
    23101 ccaacggctt cgataaacat ccccagcgcc gttttgcggg tcaacagcca gaacaggaga
    23161 agcgtcagca ccgcgataat gaccggcgtt ggcaggaaca acagcgatcc gctgccaaac
    23221 catgagagat ccggcgagtt aaacgtgacg atctgcccgg cggtgatcag ttgcgccacg
    23281 ccgcgcccgg cgaccatcag gatcagggtg gcgacaaacg gctgaatttt gaggatcgct
    23341 accagtatgc cgttccacaa tcccgccagg atgccggtgc ccagggcgct taacaaaaca
    23401 atcggcaggc tgaatcccgc gaccgtcatc gcagccgttg tggctccggc gatagccatc
    23461 accgccccta cggagagatc aatcccaccg gtggcgatca ccagcgtcat accaatcgcc
    23521 agtaacgcaa cgggtgctgc acggttaaga atgtctatgg ggctaccgaa caaacgccca
    23581 tcctgaagca ctacctgcca gaaatgcggg gctaccaggc tgtcgaccag cagcaccagc
    23641 aatagtgcta ccagctgcga cattcccgtt ggccagcgaa agcgcctttt cggcggcgta
    23701 gtgtccggga gagattgagg catcacactg ttctccttac gccgcaatgg cgttcatgat
    23761 cgccggaacg gaaagctctg ccagcgggat ctccgccacc tgtttgcgat cgcgcatgat
    23821 gatcacccgg tcggcatagc ccaccagttc ttccagttcg gaggagatca ccagcagcgc
    23881 cagaccatcg gcgcatagcg tttcaatcag gcggatgatc tcggcgtggg caccaacatc
    23941 aatgccacgg gttggctcat cgaggatcag aaattgcgga cgagtcagta gccaacgtga
    24001 aagcaatact ttttgctgat tgccgccgga gagaaattca atcggttgtt cagttgaagg
    24061 tgtgcgaatg ccaagctggc ggataaagcg ttcggcaatt tcttgctgtt ctttacggga
    24121 aatgggtcgt agccagccgc gctgggcctg gagagcgagg atgatatttt cccgcaccga
    24181 ggcggcagcg atgatgccat cggttttcct gtcttccggg cagaagccga tgcccagtac
    24241 cgaagcctga tgtggcgatc gcagggtttg cggtttgcct ttgatcaacg ccgtgccgct
    24301 gtcagcaggt ttgataccga agatcacttc ggcggtttcg gtacgtcctg atcccagcaa
    24361 gcctgccagg ccgacgatct cgccggggcg cacttcgaga tcaaacggtg cgatcgttcc
    24421 ttttttgccg taatttttga acgcggcaac gggtttgtcg ctcaacaatg ttcgcccggc
    24481 acgctgtagc gcgtgggtat ccagctcgcg ccccagcatc atttttacca gttcgatctg
    24541 cggtagctcg cgcgtttccc gacagcctac gaaactgccg ttgcgtaaga cggtgatccg
    24601 atcgctgacc tgatagacct gatcgagaaa gtgagtgaca aagatcaggc tgacgccgcg
    24661 atcgcgcaac tgacgcatca ggccaaacag taattctact tcctgagtgt cgagactggc
    24721 ggtgggttca tcgaggatca gcactttggc agagagatcg atagcccggc aaatggcgac
    24781 gatttgctgc atcgcgactg aaaagcggtt aagcggttca cgcacgtcga gggagaaacc
    24841 gtaagatgtc atcagttcgg tggcacgctt ttccatctct ttgcggcgta gaaagccgaa
    24901 acgtttgggt tcgcgaccta tgaatagatt atcagcgacc gacatattgg gtagcaggtt
    24961 gacttcctga taaacggttc cgatgccgag ttgttgtgca tgggcggtat ttttcggtga
    25021 gatagcctgg ccttccagcc agatggtgcc gcgatcggcg tggtatacac cggttaatgc
    25081 tttgattagc gtcgattttc ccgccccgtt ttcaccgagc agcgccatga tttcgccacg
    25141 gcgcaggctg aaatcaacgt tgtctaacgc tttgacgccg gggaaaaatt tacttaatcc
    25201 ttcggtgcgg aggatttcct ggtgttggtc ggtcgtcatg gtttccccct tagccacgct
    25261 ctaaaagtct gggaatagat cgtgcctgat gttaagagct gcgccgtttg tccccctcac
    25321 cctaaccctc tccccgaagg ggcgagggga cagattgtgc acggtgttaa gtccggtgtt
    25381 gcaccactgc cggatgcggc gtgaacgcct tatccggcct acacagagca ccgaactcgt
    25441 aggcatgata agacgcggta agcgtcgcat caggcttcag cgcaccaccg ccggatgcgg
    25501 cgcgagcgcc ttatccggcc tacgaatcgg catcaggcat agcaaccctc aataccccat
    25561 atttttcttc ttctctaact cttcttttgc ggtatcaggc aggtaaaggg tggatttggt
    25621 taacgtcagc ttttcaggca tggtgccgtc ttttttgtat ttctccagcg cgtcaaaagc
    25681 gggtcccgcc atattcggcg tcagttcaac actggcgttc gcttcgccat caatcatcgc
    25741 tttgtagatg tccggtacac cgtcaatgga acccgtgagg atatctttgc ccggtttcag
    25801 gcccgcttct ttaattgcct gaattgcgcc aatcaccatg tcatcgttat gggcgtaaac
    25861 catgcagatg tttttgccgt tgttttccgc tttgataaag ctttccatga cttctttgcc
    25921 tttactgcgg gtaaagtcac ctgactgcga gcggataatt ttgatatttg gcgcattctt
    25981 aatggcttcg gcaaagcctt tcttgcggtc aatggcgacg ctggccccaa cggtgccctg
    26041 cagctccacc acgttgcatg gtttgccatt cacttctttt accagccagt caccgatcaa
    26101 cttaccttcg aggatgttgt cggcagtaac ggtggtcata tagagagatt tgtctttcac
    26161 atcgatggaa cggtcgagca agaagaccgg gatttcggca tctttcgcct cttttaatac
    26221 cggttcccaa cctgtcgcga ccaccggagc gataaagatc gcatccaccc cttgcgcgac
    26281 gaaggaacgt accgctttaa tctggttttc ctgcttttgc tgaccatcgg caattttcag
    26341 cgtaattccg cgtttttcgg cctcactttt cgccacattg gtttctgcag cgcgccagcc
    26401 ggattccgat ccgacctgcg aaaatcctac ggttaatgga gcggccaacg ccatagacga
    26461 catggctgcc gagactgcag agactacaag taagcgtttc cacatagggt tgtcctcgtc
    26521 ggggtaatta ttattggtga aaagatgttc gcggaaaaac tatagacaat tcgttatgta
    26581 acggattgcg ttacatcaca cttcataaaa gtaaataaaa cgttaatcac aagtttgtaa
    26641 tcgctttcat ctcactatga aaaatgcggc tacggttatg gattttcctg ctctgtatac
    26701 cgtcttaaaa ctggcgaaaa aggaaaatga agacgaaaac aagcgaagac attcggcgcg
    26761 agttggctat aatactcggc acttgtttgc cacatatttt taaaggaaac agacatgagc
    26821 ttactcaacg tccctgcggg taaagatctg ccggaagaca tctacgttgt tattgagatc
    26881 ccggctaacg cagatccgat caaatacgaa atcgacaaag agagcggcgc actgttcgtt
    26941 gaccgcttca tgtccaccgc gatgttctat ccgtgcaact acggctatat caaccacact
    27001 ctgtctctgg acggtgaccc ggttgacgta ctggtcccga ctccgtaccc gctgcagccg
    27061 ggttctgtga tccgttgccg tccggttggc gttctgaaaa tgaccgacga agccggtgaa
    27121 gatgcgaaac tgattgctgt tccgcacacc aagctgagca aagaatacga tcacattaaa
    27181 gacgttaacg atctgccaga gctgctgaaa gcgcagatcg ctcacttctt cgagcactac
    27241 aaagacctcg aaaaaggcaa gtgggtgaaa gttgaaggtt gggaaaacgc agaagccgct
    27301 aaagctgaaa tcgttgcctc cttcgagcgc gcaaagaata aataagttct tatagcgcaa
    27361 taaccctgaa caccgggctt cggttagtaa gggttttttt atgcccgcga taaataaaca
    27421 caccttattc caccaccgcc tgcaagcgta ataacgcctc ttccaccacc tcatccgcag
    27481 ccagaccaat acgctttgcc agccgggcgc gtagatccat catccgcacc tgattcacca
    27541 gcaccacgcc gtgcacatcg ccttcttcgc aatgtaaagg aacgctaaat ccggcataac
    27601 gggcaaaatt tccgccctgc gtaatggggg ccaccagcgt cattcccagt tgattaaagg
    27661 cttgaacgga gagcacaagc gcaggtcgac cagcaccttg ctgttcatgg ccgcttgctg
    27721 gatcaaagcc aaccagcaca atgtctcccc gttcaaattc acttttcttt accatatttc
    27781 gtcacccgca ggggtggatt taccccagac atcctgctcg ctaagttccg cggcgttcat
    27841 gtcacactgt gccagcagtt catcaagcga gtagcgcctg gagatgggtg tcagaatcag
    27901 ttggttgttg ctcacctgcg cttccacgct ctgccccggc tgtaagttaa gttctttcat
    27961 tacgatattg ggaatgacca tacctgcact gttcccccat ctttttatgg taatacgcat
    28021 ctgcacctcc ttaagttata caaagtataa ctcacggtgc tacaggaaac gttttacccc
    28081 tgcacatttg tgaacgccag cagagttttt tatgcgattg cagatatgga aagcggctcg
    28141 gagagaaaag caaaagatgg aaaagttaca aaaaaaaatc gcgagataca tcgcataaaa
    28201 caacgccacc cgaaggtggc gtgcttttat tattacttat ccctgtctaa ccagtcgccg
    28261 ctttcaatta gctttaatcc atcgacgggt cgttgataaa cgtacatcca tgcactcccg
    28321 tacggcgtct gaatcaactg gcgcgcgtat tcaccgcccc tggtgcgcaa ggcatcaagt
    28381 tcggccagcg tggcgttgtc aatacgataa acttcaccgt gtactgttcc gttccccgga
    28441 actgcgcctg gatagtggcc caggctatac aactggtagt tatcgatact gaaatcgccc
    28501 agtaactggg cattggtcat ccagtgactg ttgccttgtt tgtggcgtaa actgccgtag
    28561 acaaatattc gcattgctaa aactcgaact gatagagcaa atccagtgcc tggtctacac
    28621 cagacacggc ttccagatat agcttaggca tcaggcgata acgtaacgtg agtgttgcta
    28681 tagagtcaaa tatacccacg ccgtatttca cttgcagacc tggcaataca tagccgctga
    28741 ccactacctg ggaggagtcg cctactccct gggtgtcgag cgctaaattg cttacgccaa
    28801 acgtctcgcc gattttaccc acaatctggc cactttgcgc aacccccagg ccaatcagca
    28861 ttgaggtcat cgccgcactg tcgctctgat cgctctccag cccttgtcca cgtagcaaat
    28921 aagacaatgc agcttgttgc gacatagccg ggtcagagaa gatctccgct ttcggttcgt
    28981 ccgccagacc agtgacgcga actccggcga ttacgtcgtc ttctgtagca tccgggttac
    29041 gaatagcttc aatattaaga tacggttgat ctggcggacc agagaacagt agctcaccct
    29101 tacgcacaat cagatcctga ccataggcat ggaagcgccc ttcagggatg ttgatctgcc
    29161 cgttcaggcc cagcccttgt ttgtcctgaa cgacgttaag atcgcccgtc agccgcgctt
    29221 tcaggccaaa ggcgtcaatg cgcacattgt tgccaacgtg gacaatcagg ttactgttaa
    29281 tcggaatcga cgccgttttc ggctcttccg gttgcaggtt atcgttaagc atcaccacat
    29341 cgctggagac gcctactgcg ctttccggca gatcgtgcac cacgatgcgc gcccacggga
    29401 catccacgcg accatcgagg gtaaacaggt ttggtgtagc ctcgaataca acatctggcg
    29461 atacatccat tcgtaccatc ggcggcacgg tgatccgcac tttactgcct ttcgccgtta
    29521 ctcgtgcccg ccagttttca atttggctcc agtcggcatc accgttcagg tagatttcac
    29581 cctgctgggt ccgtactgta ccggcaagcg tcgagcgcat accgttaaag ttgaccgcaa
    29641 gctggctcgg ctgcatatca aacggcataa agttgccgtc gatatccaca cccgtaacct
    29701 gaagctgacc aaacaactgc gggctttgca catcaccacc cagacgcaag ttggcactca
    29761 ccatccccgc tgctttttcc ccacaggtaa agatggggtt tatcatcgca aggttgaagt
    29821 tacggatatt gacgttgcca ccaagattac ggcggccttg cggatcggtc acctgcacct
    29881 gtccatcaaa ctggccgtta ttggtcaggc ggatggtcca gcccaattcg gcacggttgt
    29941 tacgcaattc cgccgtcaga ttcagtgtct gaaacgccac cggcagcgcc gcatcgttga
    30001 cggtttgcgt tacctgcacg ttacgccccg aaagggtgat actgccctgc ggcagcccct
    30061 ctttcgtggt gtcccaggca acatccgctt tacccgtgaa gataccgctg gcctgagtgg
    30121 tttctggcat aaacggtttc agcatggcga ggtcgaagcg gttgagattc accaccgcac
    30181 gcccttcggc ccccgcatcg atagtttgcg gcacgcacag ttccgcattc gggttaagcc
    30241 agcagtgtgg cccgatgctg attttttgct ccttattgcg gtaatccagc gcaatatcgc
    30301 gggtcagcga ccacgggccg accggcgtct ggaagcgggt attgctaaga gttcccttcc
    30361 agcgttcttc tttgcgatca aaacttcctg ccagattaag ctgcccggag acaggctcgc
    30421 cctgaatccg caactgtagc tcgtgctgct tttcgctgcc tttggcattc agggtgacga
    30481 ggttgatatt tacatccggc tgcgaaattt gctcaacgcg tacgtcgagt ttcccggcga
    30541 tctgatcggt ggatttaatg tcgccttcca cgcgaacctg cgccacggaa agttcctgcc
    30601 agcgcaggcc gcgcgcggtg atatctgcca gtagttgtgg cgcttccacc gtgccgcgta
    30661 ctttcaccag ccctttcgct gtaccgccaa gccccggcag cgcgttatcc agccccggcg
    30721 cgttgatggt ggcatcaaga ttgagatctt ttacccccag ctcgcctttc acttcggcac
    30781 tgtttggccc gagttccaga tgaagcccag ggatcatcca ctgcatataa ctgttgcctt
    30841 tcagcgtgcc gtcaacgttc actttgttct gtttgacgtt accggtcagc ttcagttctg
    30901 gcacctccat ctgccaggtg ccgccgtaca ggctaccgcg ggttttaatc aagccattga
    30961 gtttcgacgg ccactccggg aactctttgg cggtgttaat gccattaagc gttagctcac
    31021 cgcgccaact aatggcctgc tgccagtcga gcaacgcctt gagttcagtt ttcccttcca
    31081 gcgccgcgac ggtgagtttg tcgagattga cctgctgttc attacctttg gcatcaaggg
    31141 taatggtggc tggcgggatc tcctgtccct tcactgccgt acgcatagag agcgtgtaat
    31201 cggtcatttt gccggtaagt ttcagtttca gatcatccgc ctgatactgc ttctcaccag
    31261 tgaagggcca gtaaagctgt ttgctgttca cttccacgtt gagcggcaat ccggcttccg
    31321 ccagtcgcgt ctgggcgcgt aaatccatat ccaccggacc ggaaagatta acgccaatct
    31381 ccagctgttc gcgcagcgcg ccgcccactt ttagcttcac tttttcacct ttcaacggct
    31441 ccacgttcag tgtgctgttg agggtgatat ccaccggcca gttgtctgac agctgcgccg
    31501 tgccgctggc gttgacgatc ccctggttgg aatcgatatc cagggcgtcc agtttagtat
    31561 tgccgtcaat gctgctcact ttcagcagca tggtgcgcac ggtgatgtcc gtgtcgcccg
    31621 tcacgcgtaa ctgctcgcct ttaaactctt caatgttcag gttaagcggc aaatgcaagt
    31681 cggtcatttc cggcaatacc gggcgagaaa agagatcttt cagcgtttcg ccgagcggct
    31741 tttcctccgg ctgcggattt tcaattttcg gttcgaccac ttcttcctgc gccacttccg
    31801 ccactttcgg cagagcaatc agcaggcctt tcagcgacgt cggtttcagg gtcagggttt
    31861 tctcctgcca gttcaggccg gaggtgaagt ccatcaccga caccgtggtg tcatcaatct
    31921 tgatgttgac gttgtccagc gccacccgtg tcagggtgat gggatacggc gtggagagat
    31981 ccagcggacc gctatcttcc tcttcttcaa cctgttcaga aggaggcatt tttttactgt
    32041 cgatgttgac ctgaatgtct ttcagcgcca ggtcattaat acaaacgcta ctgttccaca
    32101 ggcactcaag accgaccgcc agatgcagat tacccgcttt taccgccacg cctggctgct
    32161 cataacgaac gtcagacaag gtgagatcgc gccagccgcc ggtgaccttg ccaatatcca
    32221 gtcctggcac ccagcgatcc gccgctttaa atacaagatg caggccgctg gtggtgccca
    32281 ccagaaacgc caccgatccc agcaacagta agataacgat aaccacgccg aggctgattt
    32341 ttttccataa actcataatt ctggccccag accgatgtaa aactgtaacc cgtgttcgtc
    32401 tttatccgcg accggtacgg caaaatcgag tttgattggc ccgaccggcg attcccagcg
    32461 tacgccgacc ccggtaccgg ttttaaagtc gctgcggcga atatcgctta ccgcttcgcc
    32521 actatcgaca aacaccgcgc cccaccattt tccggtcacg ttgtactggt actccagcga
    32581 tccggttatc aacttcgagg cccctttcag gtcaccgttg gcgtatttcg gggcgataga
    32641 tttgtatttg tagccgcgaa tactgcgatc gcccccggcg aagaaacgca gatccggcgg
    32701 tactttgtcg aaatcaccgg tttcaatcca gcccagcgtg ccgcgtgtaa caaaacgatg
    32761 gcgatcgtac agtgtgcgga tccagacgtt ttgtgcctgg aaaacggaga aatcgacatc
    32821 tgagccccag gccgtgttgg agtagtcgat agagtagcgt tgcgagtcgc cccaggttgg
    32881 catcaagcca ccacgagaac gcgtgcggct aatcatcacc cctggataaa acagcatcgt
    32941 ggtgttggta atttcaccct gagtaaagtg gtcgagactc cagcgcaggt taatggcacg
    33001 ctgccagccg ctggagagat cccagtagcg agaagccacc agcgtagtgg agtcagactc
    33061 ggtatcgttc aggtcagtgc gcttaaaacc gccctgcacc aaataatatt gttccagtgg
    33121 attcttcagc agcggcattt tatagctgaa gtcgagggtc tgttccggcg cggaaatact
    33181 ggtactggtg gtcagactgt gaccataaga gttcatccac ggctttttcc acgtcgcttt
    33241 cacgcgcggt cccacgtccg tagagtaacc gaccccggtt tcgatggtat tttcagttcg
    33301 cggcgaaacc acgcccgtca atggtaatac tttcgtttcg cgcgctttat caaattgtgg
    33361 agccactacg accgagttaa accagccggt agcagaaagt cgacggttca gttccgccag
    33421 atctttcgat tcgtactcat cgccctcttt aaacggcacc agattttgca ggtattcatc
    33481 gcggatttgt gatccttcaa aggtcacatg cccaaagcgg taacgttcgc cactgttata
    33541 atcaatatcc cagaaggctt tatgcaggcc gagcgcaatg cccagctgcg ctttggtaaa
    33601 ttcgctatcg aaataacctt tacgcaacgc aatgctggtt aaggactttt tgaaattttc
    33661 ataatcgccc tggttcagta ccgtgccaat agccgggcga gtatcgagca atttcaaata
    33721 gtctttatcg gtccgcgcgc cgccgcgcaa taccacatcg gtgccgccaa ttaacaccgg
    33781 cacgcctggc gtgactttgg cgatcaatac ctgccgccct ttctttggcg gtggacggag
    33841 atcaaattca atggtcggct ggtaataacc cagcgctttc agaccttcgc ggatggcatc
    33901 atcgacgcgt gcgcgaaagc gacggtctgg cgtcacttca tcactttcaa tcgtagaaag
    33961 ctgcgcacga acgttctttt ccagctgtcc cgataacccc tcgacctgta gacggacgtt
    34021 cgcggcgacg gcagatccgc ttaagcagag taagcttaca cagcataact gtcggatata
    34081 gcgcacattt tctcctgaat atcctttttt cctgcccctg gaacgccgtt aaacggctta
    34141 acaaaaatcc agtaatatgg attaaaaagc agactaaacc ccaaatattt cttatgtttt
    34201 actttagacc tattcacggt ggttattgtg tgcaaatacg cctcttgtta caaccttaac
    34261 cccaatgacc gattttcggg agagcgacac catgagttta tttgataaaa agcatctggt
    34321 ttcccccgcc gatgccctgc ctggacgtaa caccccgatg cccgtagcca cgctgcatgc
    34381 ggtcaacggt cactcaatga ccaatgtacc tgacggaatg gagattgcca tttttgcgat
    34441 gggttgtttc tggggtgtgg agcgtctgtt ctggcagtta cccggcgttt acagcaccgc
    34501 cgcaggctat accggcggct atacgccaaa tccgacttat cgggaagtgt gctccggtga
    34561 tacgggtcat gccgaagcgg tacgcattgt ttacgatcct tccgtcatca gctatgagca
    34621 gttgctacag gtattttggg agaatcacga tcccgcccag ggaatgcgtc agggcaatga
    34681 ccacggcacg cagtatcgtt cagcgattta tccgctgacc ccagaacagg atgccgcagc
    34741 tcgcgccagt ctggaacgtt ttcaggcggc gatgcttgcc gccgatgatg atcgtcacat
    34801 caccacggaa atcgctaacg ccacaccgtt ttattatgcc gaagatgacc accagcaata
    34861 tctgcataaa aacccgtatg gttactgtgg aattggcgga attggcgtct gtctgccacc
    34921 ggaagcatag cgttacgggt acaaatgtag attgttgata aagtgcgctt tatttatgcc
    34981 agatgcggcg taaacgcctt accaggccta caaaatcgtc caaattcaat atattgcaag
    35041 gactgcgtag gcctgatcgg catagcgcat caggcagtat tgcatttatc agcagtctga
    35101 attttaaccc tctggcgact ttacagtacc ttacgctata ctagccactg aaaatgccgg
    35161 ttcactttct tcgaatcggc tttcaatgtg tatttcacac aaattaatca acttcccctt
    35221 ccgaggatct ggcctgaaag gtcggataag atatgttaaa cagtatttta gtcatactct
    35281 gcttgatcgc tgtaagtgcg ttcttctcga tgtccgagat ctcacttgcc gcctcacgca
    35341 aaatcaaact taaactgctg gctgatgaag gcaatataaa tgcccaacgc gttctgaata
    35401 tgcaggaaaa tcccggcatg ttctttaccg tggtccaaat cggtctgaac gcagtggcga
    35461 ttctcggcgg tatcgtcggt gatgcggcat tttctccagc ttttcacagc ctgttctccc
    35521 gctatatgtc ggcagagctc tctgagcaac tgagctttat tctctctttc tcgttagtga
    35581 ctggcatgtt tatcctgttt gcggatttaa ccccgaaacg catcggtatg attgcgccag
    35641 aagctgtcgc tttgcgtatc atcaacccga tgcgcttctg cctgtacgtt tgcaccccgc
    35701 tggtgtggtt cttcaacggc ctggcgaaca tgatcttccg tattttcaaa ctgccaatgg
    35761 tacgtaaaga tgacatcact tctgatgaca tctacgcggt agtggaagcc ggtgcgctgg
    35821 cgggcgtgtt acgtaaacag gaacacgagc tgattgaaaa cgtctttgag ctggaatccc
    35881 gtaccgttcc gtcttcaatg acaccgcgtg aaaacgtgat ttggtttgat ctccacgaag
    35941 atgagcaaag tctgaagaat aaggtggcgg aacatccgca ctctaagttc ctcgtctgta
    36001 atgaagatat tgaccacatc atcggttatg tcgattctaa agacctgctg aaccgcgtgc
    36061 tggctaacca aagcctggca ctgaacagcg gcgtacaaat tcgcaacacg ctgattgtgc
    36121 cggatacgtt aaccctttca gaggcgttgg aaagttttaa aaccgcaggt gaagacttcg
    36181 cggtgatcat gaacgagtac gcgctggtgg tggggatcat caccctcaat gacgtgatga
    36241 ccacgctgat gggcgatcta gtcggtcagg ggctggaaga gcagattgtc gcccgtgatg
    36301 agaactcatg gctgattgac ggcggcaccc caattgacga cgtcatgcgc gtgctggata
    36361 ttgacgagtt cccgcagtcg ggcaactacg aaaccatcgg cggctttatg atgtttatgc
    36421 tgcgtaagat cccgaaacgc accgattcgg tgaaattcgc cggctacaaa tttgaagtgg
    36481 tggatatcga taactaccgc atcgaccagc tgctggtgac ccggatcgac agcaaggcca
    36541 ccgccctttc gccaaaactg cctgacgcta aagataaaga agaaagcgtc gcgtaacccc
    36601 cagaaacatc aacggctcct gaatcaggag ccgtcttatt actgcatagc actttggtta
    36661 agccatctct gtttgcagac gcataacctg acggttaact tcggacatca ctgacaaatg
    36721 cagcttatcc ttcacttttg ggataagaat cttaccctta tcaaattcaa aagcgccaac
    36781 gtccttgatg tataaccgtc cacggaacag gatctttacg tacttcgcca cctgaagtgg
    36841 gttgtaccgt tggaaaattt tcattgttat ctcctgctga gtattacgcc tgtgcggtgc
    36901 cacaatcggc ccaactatta tgaggcgcaa attttaatgc ttagtgacta tagactatcc
    36961 gggcaatgtt tccaccgcgt ataacttttt ttacctaaag gttacaatta ttcagaatta
    37021 tctttttacc gaagcgcggt cttcagtata agcattcatt tttcatatga tttgtgcgct
    37081 tgaccgcaaa ctggcatcac acttgcggga aattcgataa atagcacata tgattaaaac
    37141 tcagacccaa gtggtcggat cacctgcata tcataagaag gaaacaccat gaccctacgc
    37201 aagattctgg cactcacctg cctgctgttg ccgatgatgg cttccgcaca tcagttcgaa
    37261 accggtcagc gagtgccgcc gattggcatt accgatcggg gcgagttggt gcttgataaa
    37321 gatcagttta gctacaaaac ctggaacagc gcgcagttag tgggaaaagt gagagtactg
    37381 caacatattg ctggtcgcac ctcagcaaaa gagaaaaacg cgacgctgat tgaagcgatt
    37441 aaatcagcga agttaccgca cgatcgttac cagaccacca ccattgttaa caccgacgac
    37501 gcgattccgg gttccggcat gtttgtgcgc agcagtctgg agagtaataa aaagctttat
    37561 ccctggtcgc agtttattgt cgatagcaat ggcgtcgcgc gcggtgcctg gcagctggat
    37621 gaagaaagtt ccgctgtagt ggtgctggat aaggatggtc gcgtgcaatg ggccaaagac
    37681 ggggcgctca cacaggaaga ggtgcagcaa gtgatggacc tgctgcataa attaattaat
    37741 aaatagacac tctgaacccc ggattcagga acgactcgcg cggagtgtaa tccagcggtt
    37801 taccctgcca gtcgtgaacg tgcgctccgg cagccgcagc cacagcatga ccagcggcgg
    37861 tatcccaaat attcgttggc ccgaaacgcg ggtacagctg cgcctgtcct tccgccacca
    37921 ggcagaattt cagcgaagag ccgatggacg tggtctgatg ttcgccaagc tgttgcagat
    37981 actctttcag ctccgcatct gcatgggaac ggctgataac caccagcggc gggcgcgcat
    38041 cgcggacctg aatctgcttg cgcacaccgc actcttcttt ccaggccttg ccttctgccg
    38101 cgctgtacat tacgttcatt accggcgcat acaccacacc taaaatcggt ttgccatggt
    38161 caatgagcgc aatgttaacg gtgaattcgc cattacgttt aataaactct ttagtaccat
    38221 ccagcgggtc taccagccag taacgctgcc agtgctgacg gacttcccaa ccgggaggat
    38281 cttcttcaga aaggaccgga atatccggtg tcagcgtacg taaaccgtcc atgataacgg
    38341 tgtgtgcggc aatatccgct gccgttaccg gagaattgtc cgctttgctg acgacgtcca
    38401 tcggtttcgt cccgtcgtag acctgcataa tggcatcgcc tgcattccgt gcaagctggc
    38461 atacttgatc taacatttct ccacctcgtc tctgtgagcg gtgttaactt attgttttac
    38521 ttatacccta tcgttaatga atgcgccaac tgtgatagtg tcatcatttt caaagcgtaa
    38581 aattgtggca ttcttcactg ttctataagt aagacgttta ttctcccttt tctttcgtat
    38641 tcccgatgat aaaaggatgt ccctgatgat taagtttagc gcaacgctcc tggccacgct
    38701 gattgccgcc agtgtgaatg cagcgacggt cgatctgcgt atcatggaaa ccactgatct
    38761 gcatagcaac atgatggatt tcgattatta caaagacacc gccacggaaa aattcggact
    38821 ggtacgtacg gcaagcctga ttaacgatgc ccgcaatgaa gtgaaaaaca gcgtactggt
    38881 cgataacggc gatttgattc aggggagtcc gctggccgat tacatgtcgg cgaaaggatt
    38941 aaaagcaggt gatgttcatc cggtttataa ggcgctgaat acgctggatt atacggtcgg
    39001 tacactcggc aatcatgaat ttaactacgg tctggattac ctgaaaaatg cgctggcggg
    39061 agcgaaattc ccttatgtaa atgccaacgt cattgacgcc agaaccaaac agccaatgtt
    39121 tacaccgtat ttaattaaag acaccgaagt ggtcgataaa gacggaaaaa aacagacgct
    39181 gaagattggc tatattggcg tcgtaccgcc gcaaatcatg ggctgggata aagctaattt
    39241 atccgggaaa gtgacggtga atgatattac cgaaaccgtg cgcaaatacg tgcctgaaat
    39301 gcgcgagaaa ggtgccgatg tcgttgtcgt tctggcacat tccgggctgt ctgccgatcc
    39361 gtataaagtg atggcggaaa actcagttta ttacctcagt gaaattccgg gcgttaacgc
    39421 cattatgttt ggtcatgctc acgccgtttt cccgggtaaa gattttgctg atatcgaagg
    39481 ggctgatatc accaaaggca cgctgaatgg tgttccggcg gtaatgccgg gcatgtgggg
    39541 cgatcatctt ggggtggtcg acttacaact cagtaatgac agcggtaaat ggcaggtgac
    39601 gcaggcgaaa gcggaagctc ggccgattta cgacatcgct aataaaaaat ccctcgcggc
    39661 ggaagacagc aagctggtag aaacactcaa agccgatcac gatgccacac gccagttcgt
    39721 cagcaagcca atcggtaaat ctgccgacaa tatgtatagc tatctggcgc tggtgcagga
    39781 cgatccgacc gtgcaagtag tgaacaacgc gcaaaaagcg tatgtcgaac attacattca
    39841 gggcgatccg gatctggcaa aactgccggt gctttcagct gccgcaccgt ttaaagttgg
    39901 tggtcgcaaa aatgacccgg caagctatgt ggaggtggaa aaaggccagt tgaccttccg
    39961 taatgccgcc gatctttatc tctaccccaa tacgctgatt gtggtgaaag ccagcggtaa
    40021 agaggtgaaa gagtggctgg agtgctccgc cggacagttt aaccagattg atcctaacag
    40081 cacgaaaccg caatcactca tcaactggga tggtttccgc acctataact ttgatgtgat
    40141 tgatggtgtg aattatcaga ttgatgttac ccagcctgcc cgttatgacg gcgagtgcca
    40201 gatgattaat gccaatgcgg aaaggattaa gaacctgacc tttaatggca agccgattga
    40261 tccgaacgcc atgttcctcg ttgccaccaa taactatcgc gcttacggcg gcaaatttgc
    40321 cggtacgggc gacagccata tcgcttttgc ttcaccggat gagaaccgct cggtgctggc
    40381 agcgtggatt gctgatgagt cgaaacgtgc gggggaaatt cacccggcgg cagataacaa
    40441 ctggcgttta gcaccgatag ctggcgataa gaaactggat atccgtttcg aaacttctcc
    40501 gtcagataaa gccgcagcgt ttattaaaga gaaagggcaa tatccgatga ataaagtcgc
    40561 gaccgatgat atcgggtttg cgatttatca ggtggatttg agtaagtaaa acacttcttt
    40621 ttcggcctat aaatcatcaa ccgcatccgg catttattgg cggatgcgat gctggcgcat
    40681 cttatccgcc ctacaagcca tgcaccgtag accagataag ctcagcgcat ccggcagtta
    40741 tgccgcacgt tcatcccgca ccgccaacac ctcgggcaaa ttcaactcaa tccagtccgc
    40801 cagtgcagca accttttcgc tcacctgctc gcccagcggc gtgaggctgt attccacatg
    40861 cggcggcacc accggatacg cgatacggtt aagaaaacca tcctgttcta acgcctgtaa
    40921 cgactgcgca agcatctttt cactcacccc accgattttg cgccgcaggt cgctaaagcg
    40981 atgagtacct tcgcgtagcg ccaccagaat caataccccc caacggctgg tgacgtgttt
    41041 caacacctcg cgcgacgggc actgttccgc aaagagatta ccctctttca gttgttgcga
    41101 caggctaacc tgactcattt catacttacc tttttgtacg tacttactaa aagtaagttt
    41161 aggtgttagc gtatttaaac acaagacaaa acgatggaga cttcccatga tcgctattac
    41221 tggtgccact ggccaacttg gtcactatgt tattgaatcc ttgatgaaaa cggttcctgc
    41281 cagccaaata gtggctatcg ttcgtaatcc ggcaaaagcc caggccctgg cagcacaagg
    41341 cattaccgtg cgtcaggctg actacggcga tgaagccgca ctgacatctg cacttcaggg
    41401 agtggaaaaa ctactgctga tctcttccag cgaagtgggt caacgtgccc cgcagcatcg
    41461 taatgttatt aatgccgcaa aggcggctgg cgtgaaattt atcgcttata ccagcctgct
    41521 acatgcagat acctccccgc tcggcctcgc cgatgagcac atcgagacgg agaaaatgct
    41581 ggctgattct ggcatcgttt acaccctgct gcgcaacggc tggtacaccg aaaactacct
    41641 cgccagcgcc ccggcagcac tggaacacgg cgtatttatc ggtgcggcgg gcgatggcaa
    41701 aatcgcctca gcaacgcggg cagattatgc ggcagctgcg gcacgcgtga ttagcgaagc
    41761 cggtcacgaa ggcaaggttt acgaactggc gggcgatagt gcctggacgt tgacacagtt
    41821 agcggcagag ctgaccaaac agagcggaaa atccgttacc tatcaaaatc tgagcgaagc
    41881 cgatttcgcc gcggcactga aaagcgccgg actgcccgac ggactggcgg atatgctggc
    41941 ggattctgac gttggcgcat cgaaaggtgg tctgtttgat aacagcaaaa cgcttagcaa
    42001 attgattggc cgcccaacga caacgttagc cgaaagcgta agccatcttt ttaatgttaa
    42061 taactagtta attaaagtgg catcctcccg catcctctct gataatgacg ggatgccggg
    42121 agcaatcatg tctgcttcct gaactttctt ctgacagacc aatggatgcc agtaatgatt
    42181 agcggcgtgc tgtacgccct gttagcaggg ttgatgtggg ggcttatttt tgtcgggccg
    42241 ttgatcgtac cggaataccc ggcgatgttg cagtcgatgg ggcgttatct ggcgttaggg
    42301 ttaattgcgc tgcccattgc ctggctggga cgcgtgcgtc tgcgtcagtt ggcgcgtcgg
    42361 gactggctta ccgccttgat gctcactatg atgggcaacc tcatttatta cttctgcctt
    42421 gccagtgcca ttcaacgtac tggcgcgcct gtttccacga tgattatcgg caccctgccg
    42481 gtggtcattc ctgtctttgc caatctgctt tatagccagc gcgacggcaa actcgcgtgg
    42541 ggaaaactcg ccccggcact gatttgtatt ggcatcggcc tggcgagtgt gaatattgct
    42601 gagttaaacc acggactccc cgattttgac tgggcacgtt atacctctgg catcgtgcta
    42661 gcgttagttt ccgtggtctg ctgggcatgg tatgccctgc gcaacgcccg ctggctgcgg
    42721 gagaatcccg acaaacatcc gatgatgtgg gcgacggcgc aggcgctggt cacactgccg
    42781 gtttctctca tcggctatct cgtcgcctgt tactggctga atacgcaaac gccggacttc
    42841 tccctacctt ttggcccccg tccgctggtg tttattagtc tgatggttgc gatagccgtg
    42901 ctttgctcat gggttggcgc actctgctgg aacgtcgcca gccagcgatt accgacagtg
    42961 attctcgggc cgctgatcgt tttcgaaaca ctggcaggtt tgctgtacac ctttttgata
    43021 cgccagcaaa tgccgccgct gatgacgctg agcggtatcg cgctgttagt ggttggcgtg
    43081 gtcattgcag tcagagcaaa accagaaaaa cccttaactg aatctgtctc agaaagttga
    43141 catactggct gtgagttaaa taagcctctg ctacgtaagg gttatagctt ttgccttaaa
    43201 gatgcattta aaatacatct tatcttatta agaatgaggt atcagctatg gcttatcgcg
    43261 accaaccttt aggtgaactg gcgctctcta ttcctcgcgc ttcagctctg tttcgtaaat
    43321 atgatatgga ttactgctgt ggcggtaagc agacgctgtc gcgcgcggcg gcacgtaaag
    43381 aactggatgt tgaggtcatt gaagctgaac tggcaaagct tgctgaacaa ccgattgaga
    43441 aagactggcg tagcgccccg ctggcagaaa tcattgacca tatcatcgtg cgctaccacg
    43501 atcgtcaccg cgagcagttg ccggagctga tcctgcaagc gactaaagtc gagcgcgttc
    43561 acgccgacaa accgagcgtg ccaaaagggc tgacaaaata cctgactatg ctgcatgaag
    43621 agctttccag ccacatgatg aaagaagagc agatcctctt cccgatgatc aaacaaggca
    43681 tgggcagcca ggcaatgggg ccaatcagcg taatggaaag cgagcacgat gaagcgggcg
    43741 aactgctgga agtgattaaa cacaccacca ataacgtcac accgccgccg gaagcctgca
    43801 ccacctggaa agcgatgtat aacggtatta atgaactgat tgatgacctg atggatcaca
    43861 tcagcctgga aaacaatgta ctgttcccac gcgcgctggc gggtgagtaa tttgttcgat
    43921 caacgcacct ggtttacaca atcaggtgcg ttttcaaatg ctttgagtta ttcaccactc
    43981 cgggacaatc ttttgtatct actgaactgg gaaaattcat tattcagcca ataataagtt
    44041 ccttcaacgg ttactaactt atttttttca ttaatataat tacttattca tcgctcctgt
    44101 ataagccaga ataaaatcac ccattccctg gtttttacgt gtacatccag ctaccgctac
    44161 gcttccaccc acaccgaccc gccatcatgg cggttcatca gcgaccacag cacatggaat
    44221 ttctcgcctc ttttatactc cccctccatt ttcctgtccg tccattctgg catctgctct
    44281 ccctgctcca gtgtcaccgt cgcttgtcta ccttctacaa aaatcgacca ctccccgcta
    44341 tgaggacaag cttcaccgct aaaggccgct atccgctgcc ggtctgtaac atactgcgct
    44401 tcgcgctgtg gccagttgta caactcctcc ggcatgccct gtgggaaaaa tccctccggc
    44461 ggcacattga taaatcggtt tgccacccgc cgtatacgtt tccaattgca ttttgcccac
    44521 gccccgcttt cccagtaata ttcttgtatc ccagcctcgt tgacatactt cgttcgtacc
    44581 cgcccctgcc aagcctcggg aatatgttca ctccggtaca acagttgggc gctggcatct
    44641 gggatatccg gcagatacag gccagccacc gttaccgagt catcctcacg caggtatacg
    44701 ctactatcca gctcatacaa cggtaattgc atcggcaggg ccagcgggcc gtgcagactt
    44761 tcctcataat aataagtcag ttcgccgtca taccagtatc cgcccgaggt acgtctgatt
    44821 aaattattat aatgtgcacc tcgccatttc agtctgtcat actgtttttg cgcggcgagt
    44881 gacatccagt ccgacggata atcaattagt cctttcatat cactgctaat attgcccccg
    44941 gattgcagta cccgcaggtc cccctgctgt agctgaataa agtcatactg taccgacttt
    45001 aaagtactgc ggatattggg caacactcgc ccgccccaga cgacgtcaat ctgctcgcac
    45061 agcggacgac ggcgcacatc cagcggcatg gtcttgctcc actctgccac ctgtcgctcg
    45121 cacagctcga caaactcctg ccagccctca aaacgacgcc gataatattc cggcgacatt
    45181 attctttcca gcaaccagca ctcctgtgga tgtaatatag ccactttatc tcctgattta
    45241 ttaattaatt gtctattgat acttttattt cggtacataa atatttgttt gcaactgaga
    45301 cacagctata tacatatcca cttcatctat catcggtacg ctgaccgtca aaatagacgc
    45361 tgcgtttatc ggcagcgaaa gcaccgaatg cctgaaaact ggcggcatcg acattaggtt
    45421 caccgggcgg atttttcagg actttgccag accaaagaac agagcgtcca tcggtgagcc
    45481 aggtatgctg gcaattattc cgatcatcgc aatcgaactt atcgggatta aaatcggaca
    45541 gtaagcgggt ttcttggtta ttggctaaat tggcaatacg ccagaggcgg gaaaaattaa
    45601 cgcccggcag ttcggagagg atctcatcct tttctccctt ctgttcggca acataaaccg
    45661 tgcctttctc ctgctggcgg taataaaaat ctgcgcccca ggaaggtgaa atggagagag
    45721 gtaatgcggc gattaataac caactgtgct tagccatagt gaactctgca atgagtggca
    45781 aagatccacg gtaagcccgc gcctgagagc tgtcgatgac gggaagtatt tttaggaatg
    45841 tataaagaca aaggagattg ctgacaaagt gtgctttgtt catgccggat gcggcgtaaa
    45901 cgccttaccc ggcctacaaa agcacgcaaa ctcaatatat tgcagaggtt ctgtaggcct
    45961 gataagcgta gcgcatcagg caattttgca gcctgaaaga cacccgcagg tgccttagcg
    46021 gcattacttc gccatgcgtt ttttaccaat aaacagccag cccaacccca gcgcgataaa
    46081 ccacagcggg gtaaccagca gcgcctggcg agtgtcatct tccagtgtca gcagcacgat
    46141 cacgaacaca aagaacgcca tacataccca gcacatcagc ttgccgagcg gcatcttgta
    46201 gatcgacttc tcatgcagat gaggacgctg tttgcgatac accaggtacg agcaaaggat
    46261 aatcgtccag acgaacataa acagaatcgc ggaaaccgtc gtgatcatcg tgaacgcgcc
    46321 aatcacgctg ggattcacat acagcatcac cacgccgccg agcagacaga tgcaggagaa
    46381 ggtcagccct ttcgcgggta ctgcgcgctt agagagttta gcgaacgctt tcggtgcaac
    46441 accttcctgg gccaggccaa acagcatacg gctggtagag aagacgccgc tgttagcgga
    46501 agacgccgca gaggtcagca ccacaaagtt gatcacgctg gcagccgcag gcaaacccac
    46561 caacacgaac agttcaacaa acgggctttt ctccgggact accgaactcc acggcgtcac
    46621 ggacataatt acaatcagcg cgaagacgta gaacataatg atacggatcg gaatagagtt
    46681 aattgcacgc ggcagtgatt tctccggatc tttggtttcc gcagcggttg tacctaccag
    46741 ctcaatccct acgaaagcga aaaccgctat ctggaatccg gcaaagaagc cacttaaacc
    46801 tttcgggaac cagccgccat cattccacaa atgcgcgaat gacgcttcca caccagtcgg
    46861 tgactgaaag tgcatcgcca ccataaccag gccgacgaca atcagcgaca caatggcgac
    46921 gattttgatc atcgcaaacc agaactccat ctcaccgaac attttcacgg tggcgagatt
    46981 gagcgtcagc agcagcacta tcaccgccag cgaggcgacc cagtcggaga gatcggggaa
    47041 ccagaactga gcataagccg tgatcgccac cacgtctgcc ataccggtta caacccagca
    47101 gaaccagtaa gtccagccgg tgaaatatcc tgcccacggc ccgagtaaat cggaagcgaa
    47161 gtcactaaaa gatttgtatt ccagattcga aagcagcaat tcccccattg cccgcatcac
    47221 gaaaaagagc ataaaaccga tgatcatata aacgaaaatg atcgacggcc cggcaaggct
    47281 aatcgttttg ccagacccca taaacaaccc cgtaccaatg gcaccgccaa tggcaataag
    47341 ctgaatatgt cggtttgtga gattgcgccg tagcgactgt tcagccggag cctgatcatc
    47401 ggcaacgact tttacctgat ctaccatgtt tttttcttcc tgtacctgtc tgtgttgttc
    47461 aggctctacg gcctttagtc agtctatgat acgacgaatc tgtatcggga ctcatcgata
    47521 ttaggtaaca atacgcggga tgaatactaa gatttagatc taatgttaat tttatgttta
    47581 aagtgagtgt catatcacgg tgataacgcg aaacagctca caaaaataca cgcttacggg
    47641 gtctttgcaa cacaaaatag cgccgtttgg caaactgaag ggtttattgc tgaatgcctg
    47701 ctcccctctg gttcgagggg aacagaatgc gctgcttaga ggatttccag cagttcgact
    47761 tcaaacacca gggtgctgaa cggagggatg gatgcgcctg cgccgcgctc gccatatgcc
    47821 agttcctgcg ggatagtcag ttcccattta gaacctaccg gcatcagagt cagtgcttca
    47881 atccagccag ggatcacgcc attaaccgga aattcagcgg gttcaccacg agcaacggag
    47941 ctgtcaaaca cggtgccgtc gatcagttta ccggtgtaat gaacacgaac gcggtcggta
    48001 cgtgccggaa ttgcgccttc accctggttg atcacgcgga attgcaggcc agattcggtg
    48061 ctattcacac cttctttttt ggcgttttct tccaggtatt tcacaccttc agcagccatc
    48121 gcctggaaac gctgacgacg aacggcatcg gcgcgctcgt ggatttcacg cagcgcgcga
    48181 tgcaccacat caaccggaac agccggatgt ttgccttcca gcgcatcggc aatacctgca
    48241 accagtgctt ctggcagcag cccttccagg ccagattcac tcagttgttg ccctacctgc
    48301 aaaccaatgc cgtagcttgc ttgcgcttcg atggtgtcaa aagttggggt ggtcatcgta
    48361 tttcctttct tagggtataa aagtagcggg cagcatatca gccacacttc tttgggtaaa
    48421 actttgtcgc aggaatgatg acaaatcccg gggaaaaaga aacaatagca atatcccgta
    48481 tgaatataat aaaggcgggt ttaccacgga catcatagcg ttagcttatt cataggctat
    48541 gattgaggaa caagacgcgg agcaggagga aaccatgccc gggcgctttg aactaaaacc
    48601 aaccctggag aaagtctggc acgcgccgga caattttcgc ttcatggacc cgctgccgcc
    48661 gatgcatcgt cgggggatca ttattgccgc cattgtattg gtggtcggat ttctgcttcc
    48721 atctgatgat acgcccaacg cgccggtggt gacgcgtgaa gcgcagttag atattcagtc
    48781 gcaatcacaa ccgccgacag aagaacagct gcgggcgcaa ttagtcaccc cacaaaatga
    48841 tcctgatcag gtggctccgg tcgctccaga acctatacag gaaggccagc cagaagagca
    48901 gccgcaaact acccaaacgc aaccattcca gccagatagc ggaattgata atcaatggcg
    48961 ctcttaccgt gtcgaacccg gtaaaacgat ggcgcagctg ttccgcgacc acggactgcc
    49021 cgctaccgat gtctatgcaa tggcgcaggt agaaggcgcg ggtaagccgc tgagtaattt
    49081 gcaaaacggg caaatggtga aaatccgcca gaacgccagc ggcgtggtga cgggcttaac
    49141 cattgatacg gggaataatc aacaggtcct gtttacccgc cagccggatg gcagttttat
    49201 tcgtgcgcgg tagatgaagg gtcaaccaaa agccagctaa ggattgtctc tttgatgacc
    49261 ttacgtctgg cttccagtgc ctggggggaa accatactga tatgataaac caggccaagc
    49321 gtatgctggt tgatcaaata atatccccca agcgcagcaa tattaatatt cacctgcaat
    49381 gggtcgatat ctggcttaaa aatattgtgt ttcttccctt catccaataa ggattccatt
    49441 aactgcaagt gcgcatggtt aatttctaag agacgctgtg attttgcata atgcacacct
    49501 ttactttgat tttcactatg cacaattttt aaaaaccagg ggtt
//
LOCUS       DBANUU010000017        48766 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000017 DBANUU010000000
VERSION     DBANUU010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.017"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            103..1686
                     /gene="yegH"
                     /locus_tag="ACIE68_000962"
     CDS             103..1686
                     /gene="yegH"
                     /locus_tag="ACIE68_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFX5907312.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPPHKDGELEYEV"
     gene            complement(1960..3813)
                     /gene="asmA"
                     /locus_tag="ACIE68_000963"
     CDS             complement(1960..3813)
                     /gene="asmA"
                     /locus_tag="ACIE68_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFX5907313.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3835..4416)
                     /gene="dcd"
                     /locus_tag="ACIE68_000964"
     CDS             complement(3835..4416)
                     /gene="dcd"
                     /locus_tag="ACIE68_000964"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFX5907314.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4508..5149)
                     /gene="udk"
                     /locus_tag="ACIE68_000965"
     CDS             complement(4508..5149)
                     /gene="udk"
                     /locus_tag="ACIE68_000965"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFX5907315.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5467..8784
                     /gene="dgcE"
                     /locus_tag="ACIE68_000966"
     CDS             5467..8784
                     /gene="dgcE"
                     /locus_tag="ACIE68_000966"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFX5907316.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRMLLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMMAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQKFRRSFS
                     DPALSHALDRRVFHEFFQLAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(8823..9680)
                     /gene="alkA"
                     /locus_tag="ACIE68_000967"
     CDS             complement(8823..9680)
                     /gene="alkA"
                     /locus_tag="ACIE68_000967"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFX5907317.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            9814..11166
                     /gene="yegD"
                     /locus_tag="ACIE68_000968"
     CDS             9814..11166
                     /gene="yegD"
                     /locus_tag="ACIE68_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFX5907318.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11178..13118)
                     /gene="yegI"
                     /locus_tag="ACIE68_000969"
     CDS             complement(11178..13118)
                     /gene="yegI"
                     /locus_tag="ACIE68_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFX5907319.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYDHLVSQSQQLGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13115..13876)
                     /gene="pphC"
                     /locus_tag="ACIE68_000970"
     CDS             complement(13115..13876)
                     /gene="pphC"
                     /locus_tag="ACIE68_000970"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFX5907320.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVCQRLFAEAEA
                     KELAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(13873..14532)
                     /gene="yegL"
                     /locus_tag="ACIE68_000971"
     CDS             complement(13873..14532)
                     /gene="yegL"
                     /locus_tag="ACIE68_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFX5907321.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14753..14812)
                     /gene="ibsA"
                     /locus_tag="ACIE68_000972"
     CDS             complement(14753..14812)
                     /gene="ibsA"
                     /locus_tag="ACIE68_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFX5907322.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15085..15141)
                     /locus_tag="ACIE68_000973"
     CDS             complement(15085..15141)
                     /locus_tag="ACIE68_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFX5907323.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(15413..15469)
                     /locus_tag="ACIE68_000974"
     CDS             complement(15413..15469)
                     /locus_tag="ACIE68_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFX5907324.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            15748..16995
                     /gene="mdtA"
                     /locus_tag="ACIE68_000975"
     CDS             15748..16995
                     /gene="mdtA"
                     /locus_tag="ACIE68_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFX5907325.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            16995..20117
                     /gene="mdtB"
                     /locus_tag="ACIE68_000976"
     CDS             16995..20117
                     /gene="mdtB"
                     /locus_tag="ACIE68_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFX5907326.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVNDTQFELMMAIALVVMIIYLFLRNIPATIIPGFAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPELMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20118..23195
                     /gene="mdtC"
                     /locus_tag="ACIE68_000977"
     CDS             20118..23195
                     /gene="mdtC"
                     /locus_tag="ACIE68_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFX5907327.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVVVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            23196..24611
                     /gene="mdtD"
                     /locus_tag="ACIE68_000978"
     CDS             23196..24611
                     /gene="mdtD"
                     /locus_tag="ACIE68_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5907328.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVHVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            24608..26011
                     /gene="baeS"
                     /locus_tag="ACIE68_000979"
     CDS             24608..26011
                     /gene="baeS"
                     /locus_tag="ACIE68_000979"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFX5907329.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26008..26730
                     /gene="baeR"
                     /locus_tag="ACIE68_000980"
     CDS             26008..26730
                     /gene="baeR"
                     /locus_tag="ACIE68_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFX5907330.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            26921..27253
                     /gene="yegP"
                     /locus_tag="ACIE68_000981"
     CDS             26921..27253
                     /gene="yegP"
                     /locus_tag="ACIE68_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFX5907331.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27462..27758
                     /locus_tag="ACIE68_000982"
     CDS             27462..27758
                     /locus_tag="ACIE68_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFX5907332.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            27760..28056
                     /locus_tag="ACIE68_000983"
     CDS             27760..28056
                     /locus_tag="ACIE68_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFX5907333.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            28159..29520
                     /gene="yegQ"
                     /locus_tag="ACIE68_000984"
     CDS             28159..29520
                     /gene="yegQ"
                     /locus_tag="ACIE68_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFX5907334.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29850..30167)
                     /gene="yegR"
                     /locus_tag="ACIE68_000985"
     CDS             complement(29850..30167)
                     /gene="yegR"
                     /locus_tag="ACIE68_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFX5907335.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            30582..31481
                     /gene="yegS"
                     /locus_tag="ACIE68_000986"
     CDS             30582..31481
                     /gene="yegS"
                     /locus_tag="ACIE68_000986"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFX5907336.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(31563..32336)
                     /locus_tag="ACIE68_000987"
     CDS             complement(31563..32336)
                     /locus_tag="ACIE68_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFX5907337.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(32442..33482)
                     /gene="gatD"
                     /locus_tag="ACIE68_000988"
     CDS             complement(32442..33482)
                     /gene="gatD"
                     /locus_tag="ACIE68_000988"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFX5907338.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(33531..34886)
                     /locus_tag="ACIE68_000989"
     CDS             complement(33531..34886)
                     /locus_tag="ACIE68_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFX5907339.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(34890..35174)
                     /gene="gatB"
                     /locus_tag="ACIE68_000990"
     CDS             complement(34890..35174)
                     /gene="gatB"
                     /locus_tag="ACIE68_000990"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFX5907340.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(35205..35657)
                     /gene="gatA"
                     /locus_tag="ACIE68_000991"
     CDS             complement(35205..35657)
                     /gene="gatA"
                     /locus_tag="ACIE68_000991"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFX5907341.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(35667..36929)
                     /gene="gatZ"
                     /locus_tag="ACIE68_000992"
     CDS             complement(35667..36929)
                     /gene="gatZ"
                     /locus_tag="ACIE68_000992"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFX5907342.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDTANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(36958..37812)
                     /gene="gatY"
                     /locus_tag="ACIE68_000993"
     CDS             complement(36958..37812)
                     /gene="gatY"
                     /locus_tag="ACIE68_000993"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFX5907343.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(38122..39174)
                     /gene="fbaB"
                     /locus_tag="ACIE68_000994"
     CDS             complement(38122..39174)
                     /gene="fbaB"
                     /locus_tag="ACIE68_000994"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFX5907344.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            39431..40708
                     /gene="yegT"
                     /locus_tag="ACIE68_000995"
     CDS             39431..40708
                     /gene="yegT"
                     /locus_tag="ACIE68_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFX5907345.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            40705..41709
                     /gene="yegU"
                     /locus_tag="ACIE68_000996"
     CDS             40705..41709
                     /gene="yegU"
                     /locus_tag="ACIE68_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFX5907346.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREVV
                     "
     gene            41706..42671
                     /gene="yegV"
                     /locus_tag="ACIE68_000997"
     CDS             41706..42671
                     /gene="yegV"
                     /locus_tag="ACIE68_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFX5907347.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(42645..43391)
                     /gene="yegW"
                     /locus_tag="ACIE68_000998"
     CDS             complement(42645..43391)
                     /gene="yegW"
                     /locus_tag="ACIE68_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFX5907348.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(43443..44261)
                     /gene="yegX"
                     /locus_tag="ACIE68_000999"
     CDS             complement(43443..44261)
                     /gene="yegX"
                     /locus_tag="ACIE68_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFX5907349.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYSYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWPHSDRGQVDGINGPVDFNVFHGTVEELQAFVDGIKETP"
     gene            complement(44326..45126)
                     /gene="thiD"
                     /locus_tag="ACIE68_001000"
     CDS             complement(44326..45126)
                     /gene="thiD"
                     /locus_tag="ACIE68_001000"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFX5907350.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(45123..45911)
                     /gene="thiM"
                     /locus_tag="ACIE68_001001"
     CDS             complement(45123..45911)
                     /gene="thiM"
                     /locus_tag="ACIE68_001001"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFX5907351.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVATCCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(45946..46042)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(46134..46406)
                     /gene="rcnR"
                     /locus_tag="ACIE68_001002"
     CDS             complement(46134..46406)
                     /gene="rcnR"
                     /locus_tag="ACIE68_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFX5907352.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLIREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            46527..47351
                     /gene="rcnA"
                     /locus_tag="ACIE68_001003"
     CDS             46527..47351
                     /gene="rcnA"
                     /locus_tag="ACIE68_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFX5907353.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            47570..47908
                     /gene="rcnB"
                     /locus_tag="ACIE68_001004"
     CDS             47570..47908
                     /gene="rcnB"
                     /locus_tag="ACIE68_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFX5907354.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(47990..>48766)
                     /locus_tag="ACIE68_001005"
     CDS             complement(47990..>48766)
                     /locus_tag="ACIE68_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFX5907355.1"
                     /translation="EWTNKNNITLSNNQTSFTSGYSVTVTPAASNAKVNVSAGGGGSV
                     MINGVATLSSASSSTRRSAAVQFLLCLLGGKSWDACVNSYRNALAQNAGVYSFNLTLS
                     YNPITTTCKPDDLLITLDSIPVSQLPATGNKATINSKQGDIILRCKNLLGQQNQTSRK
                     MQVYLSSSDLLTNSNTILKGAEDNGVGFILESNGSSVTLLNITNSSKGYTNLKEIAAK
                     SKLTDTTVSIPITASYYVYDTNKVKSGALEATALINVKYD"
BASE COUNT        11544 a        12789 c        12454 g        11979 t
ORIGIN      
        1 ataatatgag ataaatagtg ctgcaacatt gcatttttgc cccgatttat ccatgatcga
       61 attgtgacat ttgtcataca acgaataggt tttgtactta ctatggaatg gattgccgat
      121 ccgtctatct gggccgggtt aatcacgctg attgtgatcg aactggtcct cggcattgat
      181 aacctggtct ttattgccat cctcgccgaa aaactaccgc cgaagcagcg cgaccgcgca
      241 cgggttaccg ggctgctgct ggcgatgtta atgcgcctgt tactgctggc gtcaatctcc
      301 tggctggtca ccctgacgca accgctgttc agcttccgct cgtttacctt tagcgcccgc
      361 gacttaatca tgctgtttgg tggtttcttc ctgttgttca aagccacgat ggagctgaac
      421 gaacggctgg aagggaaaga cagcaataat cccacacaac gcaaaggtgc gaagttctgg
      481 ggggtggtga cacaaattgt ggtactggac gccatcttct cactcgactc agtgattacc
      541 gccgtcggga tggtcgacca tttactggtc atgatggccg ccgtggttat cgctatcagc
      601 ctgatgttga tggccagcaa gccgttaacg caatttgtta acagtcaccc gaccatcgtt
      661 attctctgct taagcttcct gttgatgatt ggctttagcc tggtggcaga aggtttcggc
      721 ttcgtcattc cgaaaggcta cctgtacgct gccattggtt tctcggtgat gatcgaggcg
      781 ctcaatcagt tggctatctt taaccggcga cgttttcttt ccgctaacca gacgctacgc
      841 cagcggacta ccgaagcggt aatgcgcctg cttagcgggc aaaaagaaga tgcggaactg
      901 gacgccgaaa ccgcgtccat gctggtggat catggtaacc agcagatctt taatccgcag
      961 gaacggcgga tgattgagcg ggtacttaat cttaaccagc gtactgtcag cagcattatg
     1021 acgtcgcgcc acgatattga gcatatcgat ctcaacgcgc cggaagacga gatccgccag
     1081 ttgctggagc gaaatcagca tacgcggctg gtggttaccg acggtgatga cgcagaagat
     1141 ttgctcggtg ttgttcacgt tatcgacctg ctacaacagt cactgcgtgg cgaaccgctc
     1201 aacctgcggg tgttgattcg ccagccgctg gtgttcccgg aaaccttgcc gttgttacct
     1261 gccctggagc agttccgtaa tgcccgcacg cactttgctt ttgtggtgga tgagtttggt
     1321 tcagtggaag ggattgtgac attaagtgac gtcactgaaa ccattgccgg taacttaccg
     1381 aacgaagtgg aagagatcga cgcccgccat gatattcaga agaatgcgga cggttcctgg
     1441 acggcgaatg gtcatatgcc gctggaggat ctggtgcaat atgtgccgct gccgctggat
     1501 gaaaaacgtg aatatcacac cattgccggg ctgttgatgg aatatttaca gcgtattcca
     1561 aaacctggcg aagaagttca ggtgggggat tatttgctta aaacgttgca ggtggaaagc
     1621 catcgcgtgc agaaggtgca gattattccg ccgcataagg atggcgagtt ggagtacgag
     1681 gtgtgatggt atcggtcatt ttacccgata gccgaaagga ataagatgag ggaaccgcgc
     1741 cagcggcgat ttaccgccgg gagcccgggt tgccagggtg gtggcgattg agccaccctg
     1801 gcacgttcac cggcttgagc gttccatagt agcgaggaac atcaagtgaa cggaacagcc
     1861 ttcgatctta catattcaga atataatgat gccgccgcga acgcctgatg cgacgattta
     1921 cgcgtcttat caggcctaca ggacaggtca cctggtaacc tacatcttct ccagcaactt
     1981 cttcacatct ttgccattgc gggaatcttt attccgctcg gcccagtcat tcaggcgacg
     2041 tttcgcttcg tcctgtagat gtttgcgcag taactgatcc acttgcagac tgtaattgag
     2101 ttgctgccag ttgccataaa cccgcagcgg tactggcgtt tctttgagga aatcaatcag
     2161 tttgctttcc ccgttccagc cacccacgac ccgaatatca aactgggtgt cgcaggtttg
     2221 atctgccaga ttcaacatgc cttcccctgt cagcgccagc acaggcgatt gaccttgcat
     2281 gtcgtttaac gtcacgacgc catccttcaa cgtcaaatcg gtggtaaagc ggtcaagccg
     2341 cgtcacgtta tcaaagtttt cagcggcctt cacatcaccg ccattacgct ctaccgcttg
     2401 ctgaatcatc tgctggaagt tcatcccttc catgcgcgtg tcggtcattt cgacatgtgc
     2461 ctgtccttgc cagttgtggc ggaatgcgtc ggcatctatg tcagcaccgg agaaatcacc
     2521 agccagtgac atttttccgg tcaacgaaat cggatagtta aacgccttca gaatggtacc
     2581 aatctcaacg ttttccagcc gtggctggaa gtttatccgc ggatttattg atgtcgcgtc
     2641 cagcgtgccc ggcagtgaaa cctgtccacc gttaagcttg ccctgcagtt gagtaatttc
     2701 cagcaaaccc gacttgttgg tcatttgcgt ggcaacatct gtaaaattca ttccgcgcca
     2761 gcgcacgtta ctggcctgca acaaaatatc agccgtaaag ccttgcagtc cctgatatgc
     2821 cggttcatca atacgcgaag aaatgaccgg gcgcggcaat gttgattggc tctgcccctg
     2881 ctgcgcggca ccgttttcac cattcgctgt ttcattaagc gggatgaggt tgtcaagatt
     2941 cagttgcggg aattgcagtc tcagctgcca ttcaggtttc tctgtcagcg tgacctgtgc
     3001 ttgcccgctc agcgtgctgt cattggcggt caagctgatt tggttaaatg aaaggcgttt
     3061 atgtgactcc tgccactggg cctggaaact cccctgcccc tgaatacctt gttttggtaa
     3121 atctgcaccc tgcaactgcc agttaatttg ttcaatagcc gccgttaaat catgcggata
     3181 atcagaagca tctaccgtac cgttaaggga tatcgtgaga tcgcgctgat cgcgattaac
     3241 ccgcccggag aactcaaatg aaccacgatg ttggggatct tgttccattt gcaggcgaat
     3301 attgcggatt gtcacctgct cgtcatcttc atgctggaac accagcacgc tgtccgccac
     3361 cttaagactg gatatatcaa acgaccatcc gcgatcgtct gacagatccg gcaaggtatt
     3421 gtcgcgcggt gcaaccggag cgtcttcact gcgcaccgct tccgtctgcg gcgtcagttg
     3481 gatcactgcc ccttttagca tcacctgctt aacgctcagt tgatgactca gtagtggtaa
     3541 aagcgccacg tccagacgca tgttgtcggc gcgaaccagt ggctggcttg cgccctgggc
     3601 ggtgagagac attcgcccgg agaggatact aagctgcggc cagacgtgcc aacgcagtgg
     3661 cccgtcgagc tgcaattgat aaccgctacg tgcagcaact tgcttgacca tatagtcgcg
     3721 gaaatcattc ggattcacca gcaacactaa cgcagataac ccggccacca gcacgaccag
     3781 gagtatcatc agcgtcgtca gaaatcgtct catggtatcc tcaatgggcc tgaattagtc
     3841 tttatcgatt cggctggcta ccgcgccctg ctggttgcga tatttcgcat cttcacggcg
     3901 gttgtaaggt cgcgccgccg ggccggaaag cggctcaaag ctcagcgcac caattaacat
     3961 gcccggacgc agcgccagcg gcagcttacc ggagttgtag aactccagca caatgcaacc
     4021 agaccagccc ggatcgatac ggtgcgcggt gacgtgcacc atcagcccca gacgcgccaa
     4081 agaggaacgc ccgtccagcc agcccaccag atcggctggc agcgtcaccg actccagcgt
     4141 caccgccagc gccagctctc ctgggtgaag atagaacgcc tcgccctcgt cgagaacgat
     4201 ctcatcgctc atcacgcggt caagcgcggc gctcacttca tctttgggac cgctcagatc
     4261 gataaacgct gccgtgtgac cacggaaggt acgaaattta ttgcccaagc gtacatccac
     4321 cgtcgcgccg ttaatacgct ccactggcgg acgtgggttg atcgacaaac ggccttcatc
     4381 aagccaggct tcaatatctc ggtcacacag acgcatggca ttttctcctt tcgcgcatca
     4441 ctcccttaac gccaattacg tcaagggcat accaagttat cactgaacgg tacacaattt
     4501 atcaagctta ttcaaagaac tgacttattt tcgctttcaa tatatcgatc gcgatgcggt
     4561 ttttcccgcc gcgcggcacg ataatgtccg catattgttt agaaggctca atgaattgca
     4621 ggaacatcgg gcgcacggtt ttttgatatt gcgccatcac ggaatccatt gaacgcccac
     4681 gctcgttaac gtcacgcttg atgcggcgca tcaggcagat atccagcggg gtatcaacga
     4741 aaatggagaa gttaagttcg tcacgcaaac gcgcatccgt cagcaacaaa atgccttcga
     4801 gaatgatgac cttcttcggc tcaaccgtca ccgtttcttt catacgcgta tgttcaacat
     4861 agctgtaaac cggcaggtca attgccgagc cgcgtttcaa cgcttgtaaa tgctcaagca
     4921 gcaggctgtg atccatcgcg ctgggatggt cgtagttggt cttaacgcgt tcttccatcg
     4981 acagatggct ttgatctttg taatagcagt cttcgggaat tacgccgatg tgttcatcac
     5041 cgacttgctc acgtaattca cgataaaggg tactggcaat aagactcttg ccggaagccg
     5101 atgcgccagc gataccgata atgacgcact gatgagactg atcagtcata tatttagcga
     5161 cctgattaac ctggatgtta ggaagggggc gacgaagcgc caaacgcggc aattataggg
     5221 atttcatccg cctgatacca gtcgaatagc gctgccgcgc gctcagagtt tattgttgac
     5281 gaagaattcc cggtggcaaa ttacgttgat cagttttata caaggtaaaa aatgttatac
     5341 gcagttgcgc aaattatccg cctttacgtc actttatgag caattcgcat ataaaatgta
     5401 aaacttttgt actagcataa acacagaaac gaatactggc gacctggtct tgcggataaa
     5461 gcggtaatga gcaaacaatc acagcatgta ttaattgccc tgccccaccc gctgcttcac
     5521 ctggtcagtt taggtttagt ctcgtttatc tttacccttt tctcgcttga gctttcgcag
     5581 tttggcaccc aactcgcccc actgtggttc ccgacgtcca tcatgatggt ggcgttttat
     5641 cgccatgccg ggcgcatgtg gccgggaatt gcgctgagct gctcgctggg aaatatcgcc
     5701 gcatccatcc tgcttttttc caccagctcg ctgaacatga cctggacgac catcaatatt
     5761 gttgaagccg tggtcggggc agtgctgcta cgtaaattgc tgccgtggta taaccccttg
     5821 caaaatctgg ctgactggct gcgtctggca ttcggcagcg ccattgttcc gcctctgtta
     5881 gggggtgttc tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata
     5941 tgggtactgt cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa
     6001 ccacactatc tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta
     6061 gccatcacac tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc
     6121 attattgtgc tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc
     6181 ttccttacca cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct
     6241 acgccgcgta cgtacctgat gagccatatg ccgtggctac cgtttttgct gatcctgctg
     6301 cccgccaaca tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc
     6361 gaaagcgaaa cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg
     6421 ggcaccgagg gacaatggct gcaaaccaac aaagcgctct gccagtttct cgggtacagt
     6481 caggaagagc tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa
     6541 gatctccaac aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa
     6601 cgctactaca accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc
     6661 cacacggatg gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa
     6721 cgcaccgaac aggtcaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc
     6781 gggattggca tctgggagtg ggagttgaag ccgaatgttt ttagctggga taagcggatg
     6841 ttcgagctgt atgaaattcc tccacatatc aaaccgaact ggcaggtgtg gtacgagtgc
     6901 gtgctgccgg aagatcgcca gcatgccgaa aaagtgattc gtgattcgtt gcaatcacgc
     6961 tcgcccttta aactggaatt tcgcattacc gtaaaagacg gtattcgcca tatccgcgcc
     7021 ctcgccaacc gggtactgaa taaagaaggc gaagtcgaac gtctcctcgg catcaatatg
     7081 gatatgaccg aagtgaaaca gcttaacgag gcattgtttc aggaaaaaga gcgcctgcac
     7141 ataacgctgg actccatcgg cgaagccgtg gtctgtattg atatggcaat gaaaattacc
     7201 tttatgaatc cagtggcgga gaagatgagc ggctggacgc aggaagaagc attaggtgtt
     7261 ccgctcctga cggtgttgca tattaccttt ggcgacaatg gtcccctgat ggagaacatt
     7321 tacagtgccg acacctcacg ttccgcgatt gaacaagatg tggtgttgca ctgccggagc
     7381 ggcggcagct acgacgtgca ttacagtatt acgccgttaa gtactctgga cggcagcaat
     7441 attggttcgg ttctggtgat tcaggacgtt accgaatcac gcaaaatgct gcgccagctg
     7501 agctacagcg cctcccatga tgcactgacg catctcgcca atcgcgccag ttttgagaag
     7561 caactgcgta tgctgctgca aacggtaaac agtacacatc agcgacatgc cctggtgttt
     7621 atcgatcttg atcgctttaa agcggtgaat gacagcgccg ggcatgcggc gggcgacgct
     7681 ttgctgcgcg aactggcgtc gttgatgctg agtatgctgc gttccagcga cgtgctggcg
     7741 cgactcggtg gggatgaatt tggtctgctg ctaccagact gtaatgttga aagcgcgcgt
     7801 tttatcgcta cacgcattat cagtgccgtg aatgactatc actttatatg ggaaggccgt
     7861 gtgcatcggg taggtgccag tgccgggatt accttgattg atgacaacaa tcatcaggcg
     7921 gcagaagtga tgtcgcaggc tgatatcgcc tgttatgcct ccaaaaatgg tggacggggc
     7981 cgggtgacgg tttacgaacc acagcaagct gccgcacata gcgaacgggc ggcgatgtcg
     8041 cttgatgaac agtggcggat gattaaagag aatcagttga tgatgatggc ccacggtgtc
     8101 gcttcgccgc ggatcccgga agcgcgtaat ttgtggctga tttcgcttaa actctggagt
     8161 tgcgaaggcg agattattga tgaacaaaaa tttcgtcgta gcttcagcga tccggcactt
     8221 agccatgctc ttgaccgacg ggtattccac gaatttttcc agctggccgc aaaagcggtt
     8281 gccagtaaag gcataagcat cgccctcccc ctttccgttg ccggtttgag tagcgccacg
     8341 ctggtgaatg atctgcttga gcagctggaa aatagccctc taccaccacg gttattacat
     8401 ctgattattc cggcagacgc gattttagat cacgcagaaa gcgtgcaaaa actgcggctg
     8461 gcgggatgtc ggatagtgct cagccaggtg ggccgcgatc tgcaaatctt caactcgctg
     8521 aaagcgaata tggcagatta cctgctactt gatggtgagt tatgcgccaa cgtgcagggt
     8581 aatttgatgg atgagatgct gattacgatt attcaggggc acgctcagcg actcgggatg
     8641 aaaaccatcg ccgggccagt cgttttaccc ttagtgatgg atacgctttc tggcatcggc
     8701 gtcgatctga tttatggtga tgtgattgcc gatgcccaac cgctggattt gctggtgaat
     8761 agcagttatt tcgcgattaa ctgagggagc gtttgcccat tgcctgatgc gacgcatcag
     8821 gcctacaatt cgtccgttcc gtctggttgc cagccttccg tataccagat atgcaacagc
     8881 gcataagaac gccagggctt ccagcgctcg gcataacggc ggatttgtgc cggtgtcatc
     8941 cccggaaatc gctgtttaat cagataatca tccggcagaa aaacatcttt cgcctgccag
     9001 ccacgcaaag cgaaataatt cgccgtccag cgcccgatac ccggaaaagt ttgcagcgtt
     9061 ttcatcgcct gctccacatc gcccggaatt gtcattggta agctgccctc cagcgccgca
     9121 tttgccagat gaatcagtgc ctctgcccgt ttcaacggca tgcctaacgc ttttaatgcc
     9181 tgcgggtcgg ctgctgccag ccgctgaggc gtggggaagc agatatactc cggaaaatca
     9241 tccagccgtt cgccataaag ctgtacaact ttggcagtca attttgccgc catcgccacg
     9301 ctcaccagtt ggcctaaaat cgcccgcaca ccctgctcaa aagcatcaat acagccgggt
     9361 aaacgcaatc cgggccgcgc cgcgcctaac ttgcccaacg caccgttaac aatctgcggg
     9421 ttacattgca gatcaaacag gcggctcatt ttcgccagac actcagcggc aacgggttct
     9481 aaacctgcac ttaaatttat gtgcagagta tggcgggcta tatccggaat agcagtcacc
     9541 acgccgcgat attcgcccac cgccagacta cgggcataat aatcctcagc gaccgtttcc
     9601 acaccactca ctgcacgggc ggcgagaaat cccaacatcc acgaccagtc atacggcggc
     9661 tgccagttca gggtatacat cgcatctcct tattcatccg ctttcagcat aaacgttatt
     9721 cagacgctac gctttgcttt catattccgg ttgtcgcgac agcaacattt cgctaaagtc
     9781 acgccccttc ttcaccggca tggggattaa ttcgtgttta ttggttttga ttacggtaca
     9841 gcaaactgtt cagtggcggt catgcgtgac ggtaaaccgc aattactgaa aatggaaaac
     9901 gacagcacgc tgctgccttc aatgctttgc gcgccaacgc gtgaatcggt aagcgaatgg
     9961 ctgtaccgcc atcatgatgt tccggcagac gacgatgaaa cgcaggcgct gttacgtcgg
    10021 gcgattcgtt ataaccgcga agaagatatc gatgttacgg cgaaaagcgt gcagttcggt
    10081 ctttcctcac tggcacagta cattgatgat ccagaagaag tgtggtttgt gaaatcacca
    10141 aaatcgttcc tcggtgccag cggcttaaaa ccgcagcagg tagcgctgtt tgaggatctg
    10201 gtctgcgcaa tgatgttgca cattcgccag caggcgcagg cgcagctgcc agaagcgatt
    10261 actcaggcgg tgattggtcg cccgatcaac ttccaggggc tgggcggcga tgaagcaaac
    10321 gcccaggcgc aagggattct ggaacgcgcg gcgaagcgtg ccggattcag ggacgtggta
    10381 ttccagtacg agccggtcgc ggctgggctg gattacgaag ccaccttgca ggaagaaaaa
    10441 cgggtgctgg tggtggatat cggcggtggt acgactgact gttcattgct gctgatgggg
    10501 ccgcagtggc gttcgcgtct cgatcgtgaa gccagcctgc tgggtcacag tggttgccgt
    10561 attggcggta acgatctgga catcgcactg gcgtttaaaa acctgatgcc gttactgggc
    10621 atgggtggcg aaaccgaaaa aggcatcgcc ctgccgattc tgccgtggtg gaatgcggtt
    10681 gccatcaacg acgtacctgc gcagagtgat ttctacagta gtgccaacgg tcgtctgctt
    10741 aacgatctgg tacgcgatgc ccgcgaaccg gaaaaagtgg ccctgctaca gaaagtctgg
    10801 cgtcagcgtt taagctatcg cctggtgcgt agcgcagaag agtgcaaaat tgctctttca
    10861 agcgtagcgg aaacccgcgc ctcactgccg tttatcagcg atgaactggc tacgctgatt
    10921 agccagcaag ggctggaaag cgccctcagc cagccactgg cgcggattct ggaacaggtg
    10981 caactggccc tggataacgc ccaggaaaaa ccggacgtta tctacctgac cggcggtagc
    11041 gcccgttcgc cgctgattaa aaaagcgctg gcagaacagt tgccgggaat tccgattgca
    11101 ggcggcgatg actttggctc cgtcaccgcc gggctggcac gctgggcgga agtggtgttt
    11161 cgttaaaaga aaatggctca gcagcggctt aaatctgcct gcgcctgagc cagtttttct
    11221 gccgcctgac gtaaaggttc catcaacggc atggtccgtg ctggagcatg aaggcggaat
    11281 cgctgcaact ctgtcgcgcc gacagtcaac gtcgattcca gccgatgtcg tttggcggtc
    11341 attttcgcca ttaccgcttg tctgtctgct ggcgttatcg cttcattcgg cctgaacaca
    11401 aaacggcgtt cacagctcgc tttccagtcg ataaccgcct gggtcagatg gtcgccaaac
    11461 cctttcactt gcttaacgcc acgacgggta acatctgcgg ctgtttcaat accaaaagac
    11521 cgtaacgccg ctttacgcgc agggccaacg ccgggaatag aggcaacatc aataaaaaat
    11581 ccctccagaa acttctgctt ctgccgttcc cttgcagtgt catgaagtgc tgccagagcg
    11641 cgtttctctt cttccggtaa cccgagaatt tcgtccttca ttttttcgag catcgtccgt
    11701 ttggcgataa aaccctccag gccgcccaac tgttggctct ggctgactaa atggtcatag
    11761 tccattttcg cgcgattaaa tgcctcacgg cgttgctgga tttctgcttt ataagctttg
    11821 cttgtcagac tgccaataat ccagatagcc gccagcacag gaaccaaaat aatataacgc
    11881 ggttctgcct gaaggccgca aagcagcagc gataatgctg acagtgcgat ctcaagcaga
    11941 atgatgtatt cgcgccgtaa caggcctaaa ggaagcggcc tgccagtcgg ttggaaatga
    12001 tcgggtaatg gcagttgcaa tgctggcggt gctactgacg ccatcaccat cgcccagact
    12061 ttcgccagca caaaatcacc gccggtggta atgacctctt cgccgagatc aataaaatag
    12121 ataacgcctt gattatccag cgtacaccac gggcagtcgg tcaaatgagc gggataaaca
    12181 tgcattgccg aaacgggaca tttctttaac tgttggcgca gagaatcaag tgccgctacc
    12241 cacgctttag ccgtcggacg cccggttgcc acgccacttt ccgtgaatgc ctgctgaaac
    12301 atggcttcaa catcactcgg taacatcgat agcggaatcg atcgtggcgg cggttttaaa
    12361 ccacgtcgct gattatctga cgcgtaggca taacggaaat gggtaatatc cgtctccagc
    12421 gcattacccg catcagagat aagcggcaca ccagaataag gatgccgacc accaaacaag
    12481 acgtgaaata tcagcaaggc aaggccaaaa ttatcgtgat tcgcggtgcg ttcaaagccg
    12541 acaaatgacg acaaggtttg tagctctggc ggcgtaaaat gcgacacacc gacttcgcat
    12601 aaatgcaacg tgccattagc gttaatctga aaggagtcgc tatcaatcaa taccactttg
    12661 ctgtcgcgac ctaccataaa actgttctgg tttacgtcac ccacgacgtg cccgtgctcg
    12721 tgaaccgtag caaaagatga agcaatattg cgcgcaacat agagtagaaa atcccacgca
    12781 caatgagggt aacgttgacg acgatgtgcc gggctataga tcatatgaat cggttcttta
    12841 ccagaaactt ttggcatcat aaaaccgata acttttccgc ctcgtccacc gtgaagcgtt
    12901 gcctgcggcc aggcgacata attcaacaat tgcgcgtcag ctgtcgcagc cataaaggca
    12961 agtttgtcct gttttaaggc gggtggcggc gtgtgataaa tcttggcgac gctatcgaca
    13021 aactcattga tatcataaac cgcgccttca ccgcctttgc ccagttcacg gcctggcgta
    13081 acgcactcac cagtagcagt atataaagtg ggtttcatgg taaccacagc gccagtgcca
    13141 acgttttatc atcatccgta cgctcgttta ccgccgggct ggacaaaaac tgctttaaca
    13201 attcaggtaa taaatcgagt tgttcctgcg ttgctgacgc cagtccattg aaaaacgggg
    13261 taaagaaagg tacatgagga gaattatcca gcatatttaa cgccagtcgc tggataccat
    13321 ccgtaaatgc tgcaacttta tgcacacgct cagtgctggt gaaagtttcc agtctggaaa
    13381 cggcatcttc atcggtaatg aagtgcgtca tattggcata ttcacccacc atcggcgtaa
    13441 gcggcaattg cagaccgtga ccaaaatcca ccaccacgcc gccatcgcca atctgcataa
    13501 tcaaggtgcc gtcaggcgat gaaatcaatc ccagaaacgt gcaggcaaaa tcgcgaactg
    13561 ctaattcttt ggcttccgcc tcggcaaaaa gtcgctgaca aacggtcagc acaatatccg
    13621 tggcgaggat atcattaagc cccaattccc caccctgcac tttttgcgac atataagcca
    13681 tcgcttcatt gacggcgagc gtcgcgcctt ctcctccctg cgaaacgcta cctgcgccat
    13741 cagcaaggaa catcaccagt aatggctgct ggtcatttag ccaggcaacc tgcatctggc
    13801 aggcatcctg gcaaggcaag tccgcgctga tgtgcgatgt tccaacagcg gatgcataaa
    13861 ccagacgcca gctcacactg acgtccagcc ttttggcgct tccagcacaa cttccgttcc
    13921 cggcgtggag cgggaaaccg aacgcagcga actggataac cagctaaaca gttcacggaa
    13981 ttgtaatcct tgcagaggca aaggctgacg aacgctgatt tgggccagcg tcttcatatc
    14041 cgccccctga acaccgatgg aaaagaaagc aaacctttta tcttcttccc cccgaaacac
    14101 tttgttggct gcagcctgcc actcatcggt tggtgcacca tcggtaatca ggaaaatcca
    14161 cgggcgataa tacgaaatac catttgcacg atactcacgt ttccgctcct ctaccatatc
    14221 cagggctttg gtaatggccg cacccattgg tgtatcgccc tgggcaaaca ggatgggcgg
    14281 aaaaaaatta gcggcgctgg taaagggttg ttccacatgc accgggccga acgtcacaat
    14341 gccgagttcc actcttttta aagccagcgg atcggcaagc agttcatcac gaaaggtcac
    14401 caatccggcg ttaagttcat taataggtct gccgctcatc gagccagaaa catccagcag
    14461 caaaatgcag gggcaacgtg gttccgggtt actggcaaaa tcgctggtgg caaacgtgat
    14521 ttgttcgctc ataagaaatc ctttcttaga gaattatcga ggtccggtat cataccgact
    14581 gcaataatga aataagagca aacgaagaaa ctttcagaat taggcccggc ggcgcagaaa
    14641 attttgctat tcgcctcgca agaacggaga gccgtcgatt gacactaagg gcggagtgac
    14701 ataatttcag gagtgagggt tagggagagg tttccccctc cccctggtgt tcttagtaag
    14761 cctggaagct aatcactaag agtatcacca gtatgatgac gtgcttcatc ataacccttt
    14821 ccttattaaa agccctcttc tccgggagag gctttcccgt ttcagcgtcc cgctgaaatc
    14881 atcggcttac ctcctttcgc cctgaatgca gtctatcgct agcgcgtgaa taaaatagtt
    14941 tcctctgtga ttattgcgta acgactcgca aagaatattt gctggcagga tcgcagacta
    15001 caaagcctgc ggattgacaa tcttatcgtg aaggcatact ttcaggagtg agggtagagc
    15061 ggggtttccc ccgccctggt agtcttagta agcggggaag cttatgacta agagcaccac
    15121 gatgatgagt agcttcatca tgaccctttc cttatttaag gccccttcct cgggaggggc
    15181 tttcccgttt cagcgtcccg ctgaaatcgt cggcttatct cctttcgcca tgcacttagt
    15241 ctatcgctga cttgcagata aaatagtttc cagtgtgatt attgcgtaac gtctcgcaaa
    15301 gaatttttgc tggcaggatc gcagacggca aagcctgcgg attgacaatc ttatcgtgaa
    15361 ggcatacttt caggagtgag ggtagagcgg ggtttccccc gccctggtag tcttagtatg
    15421 ctgggtagct aatcactaag agcaccacga tgatgagtag cttcatcatg accctttcct
    15481 tatttaaggc cccttcctcg ggaggggctt tcccgtttca gcatccggct gaaatctgcg
    15541 gcttacctcc tttcgccctg tatgtagtct atcgctaacg cgtagataaa atagtttcct
    15601 gtgttattac tggatgcgtg ctcgcaaatg tgcccgccat tcagacaatt cccgatagtg
    15661 tttcataatt cctccatttt tctcccttat tggctggcta cactagtatc attccgcgaa
    15721 acgtttcagg aagagaaact cttaacgatg aaaggcagtt ataaatcccg ttgggtaatc
    15781 gtaatcgtgg tggttatcgc cgccatcgcc gcattctggt tctggcaagg ccgcaatgac
    15841 tcccagagtg cagccccagg ggcgacgaaa caagcgcagc aatcgccagc gggtggtcgc
    15901 cgtggtatgc gttccggccc attagccccg gttcaggcgg cgaccgccgt agaacaggca
    15961 gttccgcgtt acctcaccgg gcttggcacc attaccgccg ctaatactgt tacggtgcgc
    16021 agccgcgtgg acggtcaact gatggcgtta catttccagg aaggccagca ggtcaaagca
    16081 ggcgatttac tggcagaaat tgaccccagc cagttcaaag ttgcattagc acaagcccag
    16141 ggccaactgg caaaagataa agccacgctt gctaacgccc gccgtgatct ggcgcgttat
    16201 cagcaactgg caaaaaccaa tctcgtctcc cgtcaggagc tggatgccca acaggcgctg
    16261 gtcagtgaaa ccgaaggcac cattaaggct gatgaagcaa gcgtcgccag cgcgcagctg
    16321 caactcgact ggagccgcat caccgcacca gtcgatggtc gcgttggtct caagcaggtt
    16381 gatgttggta accaaatctc cagtggtgat accaccggaa ttgtggtgat cacccagacg
    16441 catcctatcg atttagtctt taccctgccg gaaagcgata tcgctactgt agtgcaggcg
    16501 caaaaagccg gaaaaccgct ggtggtagaa gcctgggatc gcaccaattc gaagaaatta
    16561 agtgaaggca cgctgttaag cctcgataac caaatcgatg ccacaaccgg tacgattaaa
    16621 gtgaaagcac gctttaataa tcaggatgat gcgctgtttc ccaatcagtt tgttaacgcg
    16681 cgcatgttag tcgacaccga acaaaacgcc gtagtgatcc caacagccgc cctgcaaatg
    16741 ggcaatgaag gccattttgt ctgggtgctg aatagcgaaa acaaggtcag caaacatctg
    16801 gtgacgccgg gcattcagga cagtcagaaa gtggtgatcc gtgcaggtat ttctgcgggc
    16861 gatcgcgtgg tgacagacgg cattgatcgc ctgaccgaag gggcgaaagt ggaagtggtg
    16921 gaagcccaga gcgccaccac tccggaagag aaagccacca gccgcgaata cgcgaaaaaa
    16981 ggagcacgct cctgatgcag gtgttacccc cgagcagcac aggcggcccg tcgcgccttt
    17041 ttattatgcg tcctgtcgcc accacgctgc tgatggtggc gatcttactc gccgggatta
    17101 tcggttatcg cgccctgccc gtttcggcgc tgccggaagt ggactatccg accattcagg
    17161 tggtcacgct ctacccaggt gccagcccgg atgtcatgac ctctgccgtt accgcgccgc
    17221 tagaacgcca gttcgggcag atgtctggcc tgaaacagat gtcgtcgcaa agttccggcg
    17281 gtgcgtcagt tatcactttg cagttccagc taacattacc gctcgatgtc gccgagcagg
    17341 aagtgcaggc cgcgattaac gctgcgacca acttgttgcc gagcgatctg cctaacccgc
    17401 cggtttacag caaagtgaac ccggcagatc cgccgatcat gacgctcgcc gtcacctcaa
    17461 ccgccatgcc gatgacgcaa gtggaagata tggtggaaac ccgcgtcgcg cagaaaatct
    17521 cgcagatttc cggtgtcgga ctggtgacgc tttccggcgg tcagcgtccg gctgttcgcg
    17581 tcaaacttaa cgctcaggcg attgccgccc tcggcctgac cagcgaaacc gtgcgcaccg
    17641 ccattaccgg cgctaacgtt aactcggcaa aaggtagcct cgacggccct tcccgtgcgg
    17701 ttacgctttc cgctaacgac cagatgcaat ccgccgaaga gtatcgccag ctaatcatcg
    17761 cctaccagaa cggcgcgccg attcgtcttg gcgatgtcgc aaccgtagag caaggtgcag
    17821 aaaacagctg gcttggcgca tgggcgaaca aagaacaggc cattgtgatg aatgttcagc
    17881 gccagcccgg tgctaacatt atctccaccg ccgacagcat ccggcagatg ctgccacagc
    17941 tcaccgagag tctgccgaaa tcggtgaagg taacagtgct ttccgatcgc accaccaata
    18001 tccgcgcatc ggtcaacgat acccagtttg aattgatgat ggctatcgcg ctggtagtca
    18061 tgattatcta cctgtttttg cgcaatattc cggcgaccat cattcccggc ttcgcagtgc
    18121 cgctgtcgtt aatcggcacc ttcgcggtca tggtgtttct cgatttttca atcaataacc
    18181 tgacgctgat ggcgttaact atcgccaccg ggttcgtggt cgatgacgcc atcgtggtga
    18241 tcgaaaatat ttcccgctat atcgaaaaag gcgaaaaacc gttggcggcg gcgctcaagg
    18301 gcgcaggtga aatcggcttt accattatct cgctgacctt ctcgctgatt gcggtgttga
    18361 tcccactgct gtttatgggc gatatcgtcg ggcgactgtt ccgcgaattt gctattaccc
    18421 tggcggtagc gattttgatc tcagcggtgg tgtcgctgac cctgacaccg atgatgtgcg
    18481 cgcggatgct cagccaggag tcgttgcgta aacagaaccg cttctcccgt gcctcggaaa
    18541 aaatgttcga caggataatc gccgcctatg gtcgtggact ggcgaaagtg ctgaatcatc
    18601 cgtggctgac cttaagcgtg gcactcagca cgctgctgct tagcgtgctg ctgtgggtgt
    18661 tcattccgaa aggtttcttc ccggtacagg acaacggcat tattcagggc actttgcagg
    18721 caccgcaatc cagctccttt gccaatatgg cccagcggca acgccaggtc gcggacgtga
    18781 ttttgcagga tccggcagtg caaagtctga cctcatttgt tggcgttgat ggcactaacc
    18841 cgtcgctgaa cagtgcgcgt ttgcagatca acctcaaacc gctggatgaa cgtgatgatc
    18901 gggtgcaaaa agtcatcgcc cgtctgcaaa cggcggtgga taaagtgccg ggcgtcgatc
    18961 tcttcctgca accaacgcag gacctgacca tcgatactca ggtcagccgc acccagtacc
    19021 agtttacctt gcaggccacc tcactggatg cgctcagtac ctgggtgcca gagttgatgg
    19081 aaaaactcca gcaactgcca cagctttctg atgtctccag cgactggcag gacaaagggc
    19141 tggtggcgta tgtcaatgtt gatcgcgaca gcgccagccg tctggggatc agcatggcgg
    19201 atgtcgataa cgccctgtac aacgcgtttg gtcagcggct gatttccact atttatactc
    19261 aggctaacca gtatcgcgtg gtgctggaac acaacaccga aaatacccca ggcctcgcgg
    19321 cgctggatac cattcgcctg accagcagcg acggtggcgt ggtgccgcta agctcaattg
    19381 ccaaaattga gcagcgtttt gcgccgctct ccatcaacca tctggatcag ttcccggtaa
    19441 cgaccatctc ctttaacgtg ccggataact attcgctggg tgatgcggtg caggcgatta
    19501 tggacaccga gaagacgctg aatctgccgg tggatatcac cacgcaattc cagggcagca
    19561 ccctcgcctt ccagtcggcg ctgggcagca ctgtctggct gattgtcgcg gcggtggtgg
    19621 cgatgtatat cgtgctcggc attctgtacg agagctttat tcacccgatc accattctct
    19681 cgacgctacc caccgcaggg gtcggcgcac tgctggcatt aatgattgcc ggaagtgagc
    19741 tggatgtgat tgcgattatc ggcattattt tgctgatcgg tatcgtgaag aagaacgcca
    19801 tcatgatgat cgacttcgcg ctggctgctg agcgcgagca aggcatgtcg ccgcgcgatg
    19861 ccatctacca ggcttgtctg ttgcgttttc gtccgatcct gatgaccacc ctggcggctc
    19921 tgcttggcgc gctgccgctg atgttgagta ccggggtcgg cgcggaactg cgtcgtccgt
    19981 taggtatcgg catggtcggc ggtctgattg tcagccaggt gctgacgctg ttcaccacgc
    20041 cggtgattta tctgctgttc gaccgcctgg cattgtggac caaaagtcgc tttgctcgcc
    20101 atgaagagga ggcgtaagtg aagttttttg ccctcttcat ttaccgcccg gtggcgacga
    20161 ttttactgtc ggtcgccatt accctctgcg gcatactggg cttccgtatg ctgccggtcg
    20221 ccccgctgcc gcaggtcgat tttccggtga ttatggtcag cgcctcgctg cccggtgcgt
    20281 cgccggagac aatggcgtct tccgttgcca cgccgctgga gcgctcactt gggcgcattg
    20341 ccggggttag tgaaatgacc tccagtagtt cgctcggcag cacgcggatt attttgcagt
    20401 ttgattttga ccgggatatc aacggcgcag cgcgtgatgt gcaggcggcg atcaacgctg
    20461 cacaaagttt gctgcccagt gggatgccca gccgtccgac ctatcgcaaa gcgaacccgt
    20521 cggatgcgcc aattatgatc ctcacgctga catccgatac ttattctcag ggtgaactgt
    20581 acgatttcgc ctcgacgcag ctggctccga cgatttcgca aatcgacggt gttggtgatg
    20641 tcgatgtcgg cggcagctca ctgcccgccg tacgcgtcgg gctgaatccg caggcgctgt
    20701 ttaatcaggg cgtctcgctg gacgacgtac gcaccgccat cagcaatgcc aacgtgcgta
    20761 aaccacaggg ggcactggaa gatggcactc accgctggca gatccagacc aatgatgagc
    20821 taaaaaccgc cgctgaatat cagccgttga ttattcatta caacaacggc ggcgcggttc
    20881 gtctgggtga tgtggcgacg gtgactgact cggtgcagga tgtgcgcaac gccgggatga
    20941 ccaacgccaa accagctatt ttgctgatga tccgcaagct gccggaagcc aatattatcc
    21001 agacggttga cagcatccgg gcaaaattac cggagttgca ggagaccatt ccggcggcga
    21061 ttgatctgca aattgcccag gatcgctccc ccaccattcg cgcctcgctg gaagaagtcg
    21121 agcaaacgct gattatctcg gtggcgctgg tgattctggt ggtgttttta ttcctgcgct
    21181 cgggtcgcgc cactattatt cccgccgttg tggtgccggt ttcgctgatt ggtacgtttg
    21241 cggcgatgta cctgtgcgga ttcagtctca ataacctgtc gttaatggcg ctcaccatcg
    21301 ctactggttt cgtggtggat gacgccatcg tggtgctgga aaacattgcc cgtcatctgg
    21361 aagcggggat gaaaccacta caggccgcac tgcaaggtac tcgcgaagtc ggttttacgg
    21421 tgctgtcgat gagtctgtca ctggtggcgg tgttcctgcc gctgctgttg atgggcggat
    21481 tgccgggccg actgttacgc gagtttgccg tgacgctttc tgtcgccatt ggtatttcgt
    21541 tgctggtttc cctgacgcta acgccgatga tgtgtggctg gatgctgaaa gccagcaagc
    21601 cgcgtgagca aaagcgactg cgtggttttg gtcgcatgtt ggtagccctg caacaaggct
    21661 acggcaagtc gctgaaatgg gtgctcaatc atacccgtct ggtgggcgtg gtgctgcttg
    21721 gcaccattgc gctgaatatc tggctatata tctcgatccc gaaaaccttc ttcccggagc
    21781 aggacactgg cgtgttgatg ggcgggatac aggcggatca gagtatttcg tttcaggcga
    21841 tgcgcggtaa gttgcaggat ttcatgaaaa ttatccgtga cgatccggca gtggataatg
    21901 tcaccggatt taccggcggt tcgcgagtga acagcgggat gatgtttatc accctcaagc
    21961 cacgcgacga acgcagcgaa acggcgcagc aaattatcga ccgtctgcgc gtgaagctgg
    22021 cgaaagaacc gggggcgaat ctgttcctga tggcggtaca ggatattcgc gttggcggac
    22081 gtcagtcgaa cgccagctat cagtacacgt tgttatccga cgacctggcg gcactgcgcg
    22141 aatgggagcc gaaaatccgc aaaaaactgg cgacgttgcc ggaactggcg gacgtgaact
    22201 ccgatcagca ggataacggc gcggagatga atctggttta cgaccgcgac accatggcac
    22261 ggctgggaat cgacgtacaa gccgccaaca gtctgttaaa taacgccttc ggtcagcggc
    22321 aaatctcgac catttaccag ccgatgaacc agtataaagt ggtgatggaa gtggatccgc
    22381 gctataccca ggacatcagt gcgctggaaa aaatgttcgt tatcaataac gaaggcaaag
    22441 cgatcccgct gtcgtatttc gctaaatggc aaccggcgaa tgccccacta tcggtgaatc
    22501 atcagggatt atcggcggcc tcgaccattt cgtttaacct gccgaccgga aaatcgctct
    22561 cggacgccag tgcggcgatc gatcgcgcaa tgacccagct tggggtgcct tcgacggtgc
    22621 gcggcagttt tgccggcacg gcgcaggtgt tccaggagac gatgaactcg caggtgatcc
    22681 tgattatcgc cgccatcgcc acggtgtata tcgtgctggg tatcctttac gagagttacg
    22741 tacatccgct gacgatcctc tccaccctgc cctcggcggg cgttggagcg ctgctggcgc
    22801 tggagctgtt caatgccccg ttcagcctaa tcgccctgat agggatcatg ctattaatcg
    22861 gcatcgtgaa gaaaaacgcc attatgatgg tcgattttgc gcttgaagcc caacggcacg
    22921 gtaacctgac gccgcaggaa gctattttcc aggcctgtct gctgcgtttt cgcccgatta
    22981 tgatgactac cctggcggcg ctgtttggtg cgctgccgct ggtattgtcg ggcggcgacg
    23041 gctcggagct gcggcaaccc ctggggatca ccattgtcgg cggactggta atgagccagc
    23101 tccttacgct gtataccacg ccggtggtgt atctcttttt cgaccgtctg cggctgcgtt
    23161 tttcgcgtaa acctaaacaa acggtaaccg agtaaatgac agatcttccc gacagcaccc
    23221 gttggcaatt gtggattgtg gctttcggct tctttatgca gtcgctggac accaccatcg
    23281 taaacaccgc ccttccctca atggcgcaaa gcctcgggga aagtccgttg catatgcaca
    23341 tggtcattgt ctcttatgtg ctgaccgtgg cggtgatgct gcccgccagc ggctggctgg
    23401 cggacaaagt cggcgtgcgc aatattttct ttaccgccat cgtgctgttt actctcggtt
    23461 cactgttttg cgcgctttcc ggcacgctga acgaactgtt gctggcacgc gcgttacagg
    23521 gcgttggcgg cgcgatgatg gtgccggtcg gcagattgac ggtgatgaaa atcgtaccgc
    23581 gcgagcaata tatggcggcg atgacctttg tcacgttacc cggtcaggtc ggtccgctgc
    23641 tcggtccggc gctcggcggt ctgctggtgg agtacgcatc gtggcactgg atctttttga
    23701 tcaacattcc ggtggggatt atcggtgcga tcgccacatt gctgttaatg ccaaactaca
    23761 ccatgcagac gcggcgcttt gatctctccg gatttttatt gctggcggtt ggcatggcgg
    23821 tattaaccct ggcgctggac ggcagtaaag gtacaggttt atcgccgctg acgattgcag
    23881 gcctggtcgc agttggcgtg gtggcactgg tgctttatct gctgcacgcc agaaataaca
    23941 accgtgccct gttcagtctg aaactgttcc gtactcgtac cttttcgctg ggcctggcgg
    24001 ggagctttgc cggacgtatt ggcagtggca tgttgccctt tatgacaccg gttttcctgc
    24061 aaattggcct cggtttctcg ccgtttcatg ccggactgat gatgatcccg atggtgcttg
    24121 gcagcatggg aatgaagcga attgtggtac atgtggtgaa tcgctttggt tatcgtcggg
    24181 tactggtagc gaccacgctg ggtctgtcgc tggtcaccct gttgtttatg actaccgccc
    24241 tgctgggctg gtactacgtt ttgccgttcg tcctgttttt acaagggatg gtcaactcga
    24301 cgcgtttctc ctccatgaac accctgacgc tgaaagatct cccggacaat ctggcgagca
    24361 gcggcaacag cctgctgtcg atgattatgc aattgtcgat gagtatcggc gtcactatcg
    24421 ccgggctgtt gctgggactt tttggttcac agcatgtcag cgtcgacagc ggcaccacac
    24481 aaaccgtctt tatgtacacc tggcttagca tggcgttgat catcgccctt ccggcgttca
    24541 tctttgccag agtgccgaac gatacgcatc aaaatgtagc tatttcgcgg cgaaaaagga
    24601 gcgcgcaatg aagttctggc gacccggtat taccggcaaa ctgtttctgg cgattttcgc
    24661 cacctgcatt gtcttgctga tcagtatgca ctgggcggtg cgtatcagtt ttgagcgtgg
    24721 ctttattgat tacatcaagc atggtaatga acagcgatta caactgttaa gtgatgcgct
    24781 tggcgagcag tatgcgcagc atggcaactg gcgcttcctg cgcaacaatg atcgctttgt
    24841 ctttcagatc ctgcgttcat ttgaacacga taattcggaa gataaacccg gcccgggtat
    24901 gccaccgcac ggctggcgta cccagttctg ggtggttgat caaaacaaca aagtgctggt
    24961 tggtccgcga gcgccgattc cacctgacgg tacacggcga cccattctgg tcaacggtgc
    25021 ggaagttggc gcggtgatcg cctcccccgt tgagcggtta acgcgcaata ctgatatcaa
    25081 tttcgataaa caacagcggc aaaccagctg gttgattgtc gctctggcaa cgttactcgc
    25141 ggcactcgcc acttttctgc tggcgcgcgg tttgctggca ccggtaaaac gacttgtcga
    25201 tggcacgcac aaactggcgg cgggcgattt cactacccgc gtaacgccca ccagtgaaga
    25261 tgaactgggc aagctggcgc aagacttcaa ccagcttgcc agcacgctgg agaaaaacca
    25321 gcaaatgcgg cgcgatttta tggccgatat ttctcacgaa ctgcgtacgc cattagcggt
    25381 gctgcgcggt gaactggaag ccattcagga tggcgtgcgt aaattcacgc cggagacggt
    25441 ggcgtcttta caggcggagg tcggtacact gaccaaactg gttgacgatc tccatcagtt
    25501 gtcgatgtct gatgaaggcg ctctcgccta tcaaaaagca ccggtagatt tgatcccact
    25561 gctggaagtg gcgggcggcg catttcgcga acgattcgcc agtcgtggcc tgaaactgca
    25621 attttccctg ccagacagta ttaccgtatt tggcgatcgc gaccgtttaa tgcagttatt
    25681 caataactta ctggaaaaca gcctgcgcta cactgacagc ggcggcagcc tgcaaatctc
    25741 tgccggtcag cgcgacaaaa cggtgcgcct gacctttgcc gacagtgcgc caggtgtcag
    25801 tgacgatcag ctacaaaaat tgtttgaacg tttttatcgc accgaaggtt cccgcaaccg
    25861 tgccagcggc ggttccgggc tggggctggc gatttgcctg aacattgttg aagcacataa
    25921 tggtcgcatt attgctgccc attcgccttt tggcggggta agcattacag tagagttacc
    25981 gctggaacgg gatttacaga gagaagtatg accgagttac caatcgacga aaacacaccg
    26041 cgtattttga tcgtggaaga tgaaccgaag ctggggcagt tgctcattga ttatctgcgt
    26101 gctgcgagct atgcgccgac gcttatcagc cacggcgatc aggtactgcc gtatgtacgc
    26161 cagacaccac cggatctgat cctgttagat ctgatgctcc ctggcaccga tggcctgacg
    26221 ctgtgccggg aaattcgtcg tttttctgac attccgatcg tgatggtgac ggcaaaaatc
    26281 gaagagatcg atcgcctgct ggggctggag attggcgcag atgattatat ctgtaagccg
    26341 tacagcccac gggaagtggt agcgcgcgtc aaaaccattt tgcgccgttg caaaccgcag
    26401 cgcgagttgc agcaacagga tgctgaaagc ccgttgatta tcgacgaagg tcgttttcag
    26461 gcttcatggc gcggtaaaat gcttgacctg acgcctgcgg aatttcgtct gctgaaaacg
    26521 ctctctcatg aaccaggaaa agtgttctcc cgcgagcatt tgctcaatca tctttatgac
    26581 gactaccgcg tagtaaccga ccgcaccatc gacagccaca ttaaaaacct gcgccgcaag
    26641 ctggaatctc tcgacgccga acagtcattt atccgcgccg tttacggcgt tggttaccgc
    26701 tgggaagccg acgcctgccg catcgtttag ttttagcgac atgactttgt tagccggaga
    26761 tggcgttcag accagatccg gcaacattat cccacgcatg gtcagcaaac tgacattatc
    26821 tctccgccct ggcaggcatc agaaaatcgc tcatacttta atcggtaaac agcaccttta
    26881 gatgctgttt tcgatacaca atttcaatca aggagtcatt atggctggtt ggtttgaact
    26941 cagtaagagc agtgataatc agttccggtt tgtgctaaaa gcgggcaatg gtgagactat
    27001 cctcaccagc gagctgtata cctcaaaagc ctctgcggaa aagggcatcg cgtcggtgcg
    27061 tagcaacagc ccgcaagaag aacgctatga gaaaaaaacg gcaagtaacg gcaaattcta
    27121 tttcaatctg aaagccgcta atcatcaaat tatcggctcc agccagatgt acgccaccgc
    27181 gcaatctcgt gaaaccggaa ttgcctccgt taaagccaat ggcacaagcc agacggtgaa
    27241 agacaatacg taattacgat gccgggcgtg ggttgttggc gtccggcttt atatccaaat
    27301 aacgcttagt tattaataat taagagtaac cgccgccatt aaaatagtgg gcactgacga
    27361 gcactcgttg tgctttcttt gtttaccatc ccatacttat cctcctgaac atgttcatgt
    27421 gccaaaatat cttctaatac tttcgtgacg ctatccggtt tatgacatac actgatgtaa
    27481 gatttttact tacaggcggg cgtaaaatgg ccagaacgat gacggttgat ctcggcgatg
    27541 agttgcgtga atttatcgag tccttaattg aatccggcga ctatcgcacc caaagcgaag
    27601 tgatccgtga gtcactgcga ttacttcgag aaaagcaagc agagtctcgc ctgcaagcgt
    27661 tacgtgacat gctcgcagaa gggttgagta gtggtgaagc tcagccgtgg gaaaaggacg
    27721 cgttcttacg gaaggtgaaa gcagggataa ggaaataatg tgcgtattat taaactcatg
    27781 ccaaaagcca atgaggattt agaaggcatc tggtattaca gctatcacca tttcggcgaa
    27841 ccacaagccg acagatatgt ggaacatctt tcagacgttc tccagattct gagtaacaat
    27901 aatataggta cgcccagacc tgaattaggc gaaggtattt ttgttcttcc ttttgaacgc
    27961 cacgtcattt atttcctgca atcaccaggg gaaattatcg tcattcgtat ccttaaccaa
    28021 aaccaggacg ccaccagaca tttacactgg agctaaaccc gccgattgcg ctacaatgcc
    28081 cgcccttaaa gtgggggcac tcccctaacc gcttcatcag gtgaagcgga tctgacctgt
    28141 catcagaacg agagaattat gtttaaaccg gaactccttt ccccggcggg aacgctgaaa
    28201 aatatgcgtt acgctttcgc ttatggcgca gatgctgttt atgcgggcca gccgcgttat
    28261 tccctgcgtg tgcgcaacaa cgaattcaac cacgaaaatc ttcagctcgg catcaatgaa
    28321 gcccacgcgc tggggaaaaa gttttatgtc gtggtcaaca ttgcaccgca caacgccaag
    28381 ctgaaaacct ttatccgtga cctgaaaccg gtggtggaaa tggggccgga tgcgctgatt
    28441 atgtccgatc cagggctgat tatgctggtg cgtgagcact tccctgaaat gccgatccac
    28501 ctttcggtgc aggctaacgc cgtgaactgg gcgacggtga aattctggca gcaaatgggc
    28561 ctgacccgcg tgatcctctc tcgcgagctg tcgctggaag agattgaaga gatccgcaat
    28621 caggtgccgg atatggagat cgagatcttc gttcacggcg cgctgtgcat ggcctactcc
    28681 ggtcgctgcc tgctctctgg ctatatcaac aagcgcgacc cgaaccaggg cacctgcacc
    28741 aacgcctgcc gctgggagta caacgtccag gaagggaaag aagatgatgt tggcaacatc
    28801 gtacacaagt acgagccgat tccggtgcaa aatgttgagc cgacgctggg tatcggcgca
    28861 ccaaccgaca aagtgtttat gatcgaagag gcccagcgtc cgggcgagta tatgaccgcg
    28921 tttgaagatg agcacggcac ttacatcatg aactcgaaag atctgcgcgc catcgcccat
    28981 gtagaacgcc tgaccaaaat gggcgtgcat tcgctgaaaa tcgaaggtcg taccaaatct
    29041 ttctactatt gtgcacgcac cgcacaggtt taccgcaaag ctatcgatga cgccgctgcg
    29101 ggaaaaccgt tcgataccag cctgctggaa actctggaag gtctggcgca tcgtggctat
    29161 accgaaggtt tcctgcgtcg tcatactcac gacgattatc agaactacga atacggttat
    29221 tcagtttctg accgccagca gtttgttggt gagtttaccg gtgagcgcaa gggggacctc
    29281 gcggcggtag cggtgaaaaa taaattctcc gttggcgaca gccttgagct gatgacgccg
    29341 caaggcaaca ttaattttac ccttgagcac atggaaaacg ccaaaggcga agctatgccg
    29401 atagcaccag gcgatggtta tactgtgtgg ctcccggtcc cgcaggatct tgagctcaat
    29461 tacgcgctgc tgatgcgtaa tttctccggg gaaaccacgc gtaatcccca cggtaagtga
    29521 ttaatttcga ttatttttcc cggatggaaa attcttagaa accgatcaca tacagctgca
    29581 tttattaagg ttatcatccg tttcgctgaa aaacataacc cataaaatgc tagctgtacc
    29641 aggaaccacc tccttagcct gtgtaatctc ccttacacgg gcttattttt tatgcataag
    29701 ccctatccct ggtcaccgtc ttccattgac cacatcgata gaatctccct tcatagcacg
    29761 atgcctttca cgtaacggca tcgtgctcgc acaggttccg gctaagcaca accagaacgc
    29821 gcatgtttga cgcttaccaa aaaatattct cactctccac atttgaatgt cagacgagcg
    29881 acgccatgta atcctgcacc ttctgtcttc aggtcaacta tctgcatttt tttgccctga
    29941 gtaacacaga aatgggctgc atcatttttt actatatttt ctgcaccaga tattctgccc
    30001 ctggctaaag aagcttcggc ttcggtgtag tattggttat cgagtttacg ctgaatatta
    30061 cttttatatg caagaccaaa tttaccgata cttgtctcat cattatgcac agcacaacca
    30121 gacataataa aaatactaat taatgatata gcagctatct ttttcatctc accttccccc
    30181 attaaatacc aacgacactc tctagtgttt aaatataata atggcatgat tattataatt
    30241 gaataggatt ataataaatg ttctgtacaa catttcctac ataagtagga attacggaca
    30301 ttgaggccct tcagggtaac tccatggggg ctttaatata tttggaatag tatatttaag
    30361 atgccactgt ttggttgaat attaggcata tgctcttttt tgaaatttat cggtggcagc
    30421 cgttagtatt cgctgtcccc attgcaagct cctggtggta accactgaat cctccatact
    30481 tgaactgact ttttatcctc cgactttcat cctgttctga ctccaccttt tgttttctgc
    30541 tctacactat ctacagacca atcataaagg cacatacgat catggcagaa tttcccgcca
    30601 gcttactgat tcttaatggc aaaagtactg acaatctacc cttgcgcgaa gcaattatgc
    30661 tgttgcgtga ggaaggaatg acgatccatg tgcgggtcac ctgggagaaa ggcgatgccg
    30721 cacgatatgt agaggaggcc cggaagttgg gcgtcgcaac ggtgattgcc ggtggtggcg
    30781 atggcaccat taatgaagtt tctacggcgt tgattcagtg tgagggggat gacatacccg
    30841 cgctgggaat tttgccatta ggaaccgcca atgattttgc caccagtgta gggattcctg
    30901 aggcactgga taaggcgctg aaactggcaa ttgccggtaa cgccattgcg atagatatgg
    30961 cgcaggtcaa caaacaaacc tgttttatta atatggcgac aggcggattt gggacgcgta
    31021 ttaccacaga aacgccggaa aaattaaaag ccgcgctggg tggcgtctct tacatcattc
    31081 atggcttaat gcgcatggat actctgcaac cggaccgttg tgaaatccgc ggtgaaaact
    31141 ttcactggca aggtgacgcc ctggtcattg gtattggtaa cgggcgtcag gccggtggcg
    31201 gtcagcaatt gtgtccgaac gcgttaatta acgatggctt gctgcaactg cgcattttta
    31261 ccggcgatga aattcttccg gctctcgtat caaccttaaa atctgacgaa gataacccga
    31321 atattatcga aggcgcttcg tcgtggtttg atatacaagc cccacacgaa atcactttta
    31381 atcttgatgg cgaaccgttg agcggacaaa actttcatat tgaaatactt ccggcggcgt
    31441 tgcgttgtcg attaccacca gattgtccat tattgcgtta atcaaactat tctctcttga
    31501 accagggaga atccgtaatg ccggatgcgg tgtaaatacc ttatccggca tacagaacaa
    31561 tactacaccg taattaattt cacccgtgtc ttctgcaatg ccagtttatc cacctctgat
    31621 acagcatcat cggtaataat aatgtcaatt ttttcgattg gcagtacctg attaaaaccg
    31681 cgacggttaa atttcgatga atcgagaacg gcaatcactt tattagcggc tgtgaccatc
    31741 gcaccgctaa cggaataacc ctcattaaag gtcgtaatac cattaaccgc atcaatacca
    31801 tcagcaccga caaacattaa atcggcatta atatcctgca atgaacgctc ggcaatggaa
    31861 ccatgcatcg agcgcgtttt atggcggacc gtgccaccac agacgaccag agtaatgtct
    31921 ttattttcgg aaagggcaaa cgctgccggg agactgttgg taatcaccgt gatattttta
    31981 gcggtcatta atccttcggc gataagcatt gtggtacttc cgctgtcgag aataacggtc
    32041 atcccttcat ggatcatttt gactgccgcc tgcgctattc tgttttttgg cgcgctggcg
    32101 agctgaaatc gctctttaaa cccgacttcc tgggtctcgg tttcactatt accggacatt
    32161 attttcgccg caccgccatg aaagcgcgta accacgcctt tttgttcgag aaagcgcaaa
    32221 tcggcacgga ttgtcgcttc cgaggcagca aatactcccg ccagatcctg aacaagcacg
    32281 gttccctgtt cattcactaa ttggatgatc ttattccttc gctcgaatga attcatcgtc
    32341 atttacccat attcaattgt agctagtgta aacgaagggg gaccattggt gaatgatctg
    32401 ttttgcacaa taccgggcca gcatcacgct ggcccgcggt ttcagggaat gagcaacact
    32461 ttgcccggca tggcattacg agcgatgtca cgcaccgcct gggcgaagct ttcaaagctt
    32521 ccacggtgag cgattaatgg ctccaggctt aatttacgtt ctgtcagcaa ccggctcgcc
    32581 gtttcccact cctgccccgg ccaagggctg gagtagttca tccaactgcc gataaccgtc
    32641 agctctttac gcaatatttt gccaaacgtt gccgatgtta aatgcagatc ctgatgcaac
    32701 gtgcccacca gcgccagttg ggcatgagga ccggcaatct ctaccgccag ttcgacagtt
    32761 tgcggtacgc cagccgtctc gaggataagc tgattaaagc gcagttcgcg taaaacgctc
    32821 tgcatttgcg gcgcgctcat ttcgctactg ttaaatgttt gcatcgcacc gaaagatttt
    32881 gccagtgcca gtttttctga gctaatgtcg atagccgtca cactctttgc tcccagcgcg
    32941 acagcgcact gaatagccag caggccaatg gttccggcac caataataat aacgttttta
    33001 ttctcacaac cttgcgctaa atgaaaagca tgcagtccaa cggtaatcgg ctcaataaaa
    33061 gccccatcct caataggcat atccgtgggt agagcaaaga cattttttcg cttaacgaca
    33121 atatattcag caaatccacc atcacgccgc gagccaataa aatcatattt tgcgcactgg
    33181 gaataaaacc ctttcaaaca ctctggacaa gtaaaacagg gtaataacgg cacacaggca
    33241 accgcatcgc cagggtgtaa atcatcaaca ccggatccca ccgcatcaat atagccgcta
    33301 aattcatggc ctaacgttat tggataataa tgtgcaccat ttttaaatat cctgggtaaa
    33361 tcggaaccac ataagcccga gctggcaatt tttacccgta cctcatcctg atgtttaatt
    33421 tcaggaatga cgctttctgc aacgcgcacg ataccatcag tatcattcac cactgatttc
    33481 ataaaaactc ctgattgtta aggggggata aactccccct caggtaaaaa ttattctgcg
    33541 agaacgactt tctcttgttt aataaagcca cgcgctctac gccaggtcat gaaaataccg
    33601 gtcagataaa ttgcaccgat aataatgaaa ccgggaatat tttgcgggga gaaaacctga
    33661 atcagtaacc aggtaatggg agaaccgccc tgatccattg aagccaccat acccccggct
    33721 tttaacgccc cagcattagc cgccagttgg gtgtgcaaac caatagtttg cgtcgcgatc
    33781 cacagggtga tgctcataat gatgacaccc gagattaagg tgcggaacag atttccacga
    33841 tgcacggcga ccgccatcgc cacgaagaag ccgatggtgg caagatcgcc aaacggcagc
    33901 acctgattac ccggcacaca aacagcaatt aaaatggtga gtgggataaa aatcaggctt
    33961 gccgatacca ccgccgtatg tcccagcagc aacgccggat caaggccaat caggaactcc
    34021 tgaccgccga acttcgcctg taaacgacta cgagcctgct tagcgatggg cgttaaacca
    34081 tccatgatgg gtttaatcac ccgtggcatt agcagcatca ctgccgccgt ttttaccgcc
    34141 agctgcaata cacctttgac atcgtaaccc gcgaggatgc cgataatcag ccccatcaca
    34201 aaacccacgg tgacaggctc gccaaatgga ccaaatttgc gctgaatatc gtcggcgcta
    34261 aatttaattc ggttaacgcc tgggattttc tcgatgatag catcgaccag caccgcaatc
    34321 ggccccatat acgccgacgt accgtgcgga atagcaatac cttccagctc aaagaaattt
    34381 cgggtatcgc gggcaaacca gtcgccgagc ttataaacaa acgccgcgtg aattaccaca
    34441 cctgccatcc ctatcatcca tgaaccggtt gccaggtgca gcaacgcgcc ggtgaaggtc
    34501 atatgccaga tattccagat atcaacattt accacccgcg tcatacgggt cagtaacatc
    34561 gccacgttaa ccaatatcgc aatcggaatc gccaccagcg caatttgcga cgcccaggtc
    34621 attggtgaag agcccggcca gccgacatcg accacatgca gattcaggtc gaaattttcc
    34681 gccatcgctt tcgccgccgg accaatggaa tccagcatta agccaatcac aaggccaatg
    34741 ccaacaaagc caatcccgat atgcagaccc gctttaaagc aatcgcctgc cttcatgcct
    34801 aatattttag aaaaaataat gatgacaacc ggcagcatca ctgtagggcc gaggtcgaga
    34861 atataacgca tgacttctga aaacataggt cacccctgta agatagtcag aattttattt
    34921 tgtaatgctt cgataccgac accagaaata aaaggcatgc cgtgaactaa cggaatatcg
    34981 ccaaaactac gatccacttt ggcagtggtg catatcaaat gcacaccatc catataggtt
    35041 tctatttcat taacccgaca ctggattaat tcaacaggaa tattatgatt ctgacacaac
    35101 tctttaattt cttccgccgc catcgtagag gtcgcaaccg cgcctccgca agcgacaata
    35161 atcttgcgtt tcataaatag tcctttttat tgttacagag ggatttattc gtctgaatct
    35221 aaaacatact ttgtgaagta ttcctttaac tgggtttcag gaagagtgat tagtgtctcg
    35281 acgatatcgg gctgttgtaa cttgccaaat aaacagcgta aaagtttcaa ttgctgctgc
    35341 ggattttcca caatcaacgc aataaccaac gataccgcca cgtcgttatc atcatccgct
    35401 tgctgaaaat gaactttatt tgttggcctt aacagataaa tggctgacga cttagcatga
    35461 atcgcctcac aatgcggtat tgcaatggcg tgctgctcaa gcattatccc ggtagggaat
    35521 tctgcttctc tggcaattaa tgcctgtggc caggtatcat gaaccacacc tttggcgagc
    35581 atctcattac cgatatgggt taaaacttcg ctacgatcga caaaagaaat tccgctacga
    35641 acaaacaggt tagtcatata ccgtccttat tccgcacagc cgtagcgata ggcgcgcaaa
    35701 acatcataaa ttttatccat aatcagctga tgcggtattg ctgataattc cccggactga
    35761 atgcgttcaa attgtttggg aagatactga ctaatcatgc ccagtgggat gtccacgcct
    35821 tcaagattca ccatcatcgt ttcgacgcta tttttaatcc gactatgcgg ccagtaataa
    35881 cgaatacgat ccgacaggct gtaacgaata tccagtaatg aatcgttaaa acccgtacga
    35941 taatattttt tccagtattg cggttcgtcg agcatcactt cttcaattac cgccaggcaa
    36001 ccgctgcgat tttcaggggc gataagttcc tgctcaattt gtgccagtgc aaatatcgcc
    36061 tcgcgtaaag caaaggttaa tgcgggaccg actttcaata ttgcaaagtg atcgcggact
    36121 aattcccaat aagccgtccg ggtctggtaa tcggtagaat gtgcttcata aaccattcgg
    36181 gtgttttcta tccattgcgc cagcggctgc gcttcctgcg gctgataatg gataatattg
    36241 ctgtgatcaa attccacgcc cggctgcacc acgatggcaa tcacgcgtgt taacgcctct
    36301 gtcagcccac gggcaataaa ggccttttga tgcgtacgta aagtattggc ggtatcttca
    36361 acatgggtga tgtgtactga ctgaatggcg ctggcctcac cgcccggaac cggaacttcg
    36421 gtgccaatga catagctcag ttgctcacgc tggcaatctg tcgccacact ttccgcagca
    36481 aagcaaagca cagcagctcg ttccgcaacc gtttctggtg ctaacggtat gggatccccc
    36541 gcgcaggaca ttgacgcatc aagatgaatt ttactgaagc cggcacgaac atattccttt
    36601 accagctcga cggatttttc catcgccgca tccgcatttt cttgctgcca gcagtttggc
    36661 cccagatgat cgccgccgag aataatgcgt tcgcgtgcaa acccaacttt atcggcaatc
    36721 gtaaaaacaa attcgcgaaa gtctgccggt gtcattccgg tataaccgcc aaattgattg
    36781 acctggtttg acgttgcttc aatcagcact ttgcgcgtgc tgttgcgatc aaatgccagc
    36841 gccgcttcga taaccaacgg atgggcagaa cagactgaac atatgccgat atgttcacca
    36901 gctttatgcc gggcaattaa cgttttcatg ttttttcctt attaaatggc aggtgcgtta
    36961 tgccctgccc tcgcagccac aatcggcaat cactttgctc accacatcgc gcattgcgga
    37021 tttagccgac tgcaaataat cccggggatc ggtcgcttca gggtgctcgg tcaggtaatt
    37081 ttttaacgcc tgcgagaagg catttttcag ctccgttgca acgttgattt tgcatatccc
    37141 cagtttgatg gtttgctgaa tatccttagt cgataaccct gacgcgccat gcagcaccag
    37201 cggtaagttc acccactggc gaatgttctc cagtcgagaa aaatcaagcg ccggtgcgct
    37261 ggcatacatc ccatgagccg tgccgatggc gaccgccagg gaatcaattc cggttgcctc
    37321 ggcaaattca cgcgcctgag cggggttggt ataaaaagca tcggcttcat tgacttgcac
    37381 atcatcttcc tggccgccaa gttgccccag ctccgcttcg acgctgacat caaagcgatg
    37441 gcaaaaatcc accacctctt tgacccgtga aatattttgc gcaaaaggca aatgcgaggc
    37501 gtcaatcatg actgagcgca cgccagaacg aaccttctga gcgatatcgt caaatttcgt
    37561 gtgatggtcg agatgaattg ccagtggatg gtgatattgc ttcgccatcg cgctgaccag
    37621 cgccaacaga ttttctgtac cagcatgagt aaatgtgcca ggcgttccgg cgatgatgac
    37681 cggcgcatgc aggttggcag cggtttccac caccacttgc atcgtttcga gattgtgaat
    37741 attgaatgcc ggaaccgcat aaccgccgcg ctgtgcgttg ttcagcatct gctttgtcga
    37801 taccacgtac attttcatat cctgtcgttt gttttcgatt tcaaaatata atgaaatgat
    37861 ttgtttttaa atgtcgagat aacgatcaca aaaacgacaa tgtgaaaatt gttcgaggag
    37921 taaaaggcaa aaaaaaacgg cctcccgata gggaagccgt agcaaagtgc gcgtgttttt
    37981 atgccggatg cggtgtaaac atacgtggca gtgctatgta ggcatgataa gacgcttccg
    38041 cgtcgcatca ggcataggtt gccggatgca gtgtaaacgc cttatccgtc ctacggggtg
    38101 gtgctgttcg tatgaggcgt atcaggcaat agtaattttg ctatcgagat aaacgtcctg
    38161 cacggcatta atcagtttca cgccgtcagc catcgatttc ttgaacgcct tacgtccaag
    38221 aatcagcccc attccgccag cgcgtttgtt gataaccgca gtacgcactg catcactgag
    38281 gtcagtttca ccgcctgcag caccgccgga gttaatcagc cctgcgcgcc ccatatagca
    38341 gttagctaac tgataacgca ccagatcaat cgggttttcg ctggttaact tgctgtacac
    38401 gcgatcgtcg gtataaccgt aattaattgc tttatagccg ccgttatttt ccgccatttt
    38461 ttgtttgacg atatctgcac cgatggttgc cgccagatgg tttgcctgac cggtcaggtc
    38521 ggcggaaaca tggtaatcaa cgccatcttt cttaaaggcg gagttacgca aataggccca
    38581 cagcactgtc accataccca gctcgtgcgc acgttcaaaa gccgcagaaa tttcttcaat
    38641 ctggcgacgt gactcttccg agccaaaata gatagtcgca ccaaccgcaa ccgcgcccat
    38701 gttgaacgcc tgctccacgc tggcatacag cgtttgatcg taggtattcg ggtaacttag
    38761 cgtctcgttg tgattaagtt tgacgaggaa tggaatgcga tgcgcataac gccgcgatac
    38821 cgacgccagc acgccgtaag ttgacgccac acagttacag cccgcttcga tcgccagttc
    38881 aacaatgttt ttcgggtcaa agtagagcgg gttagcagca aatgaagctc cggcagagtg
    38941 ctcaacgccc tggtcaaccg gcagaataga aagatatcct gtgccagcca gacgcccggt
    39001 gttgtacaac gtctgcatat tacgtaacac cgctggcggg cgattattgt caatcattac
    39061 gcggtctacg tagtcatgtc cggggagata aagctggtca gaaggaattg tcatacaacg
    39121 gtgctgtaaa aggttgtcgg cgtctttgcc aagcaactgc gcaatatctg tcattactat
    39181 gctcccgtaa attccgattg gatatcggct atggattgtc ctggcccgcc ttttgcgggc
    39241 aatcataatc ctggtcgtta cgggcgagtt tttccatctt ttggatgttt tttcagcgtt
    39301 ttctgttggc tcgattcatc agaaaaaatg ttagcgcggt caagttttca ccgcaaaggt
    39361 atttaaaagg tattattaag tggtattgtc atcgcgtacc ttacattacc tgtcatgaag
    39421 gaattaaaag atgaaaacaa cagcaaagct gtcgttcatg atgtttgttg aatggtttat
    39481 ctggggcgcg tggtttgtgc cattgtggtt gtggttaagt aaaagcggtt ttagtgccgg
    39541 agaaattggc tggtcgtatg cctgtaccgc cattgcggcg atcctgtcgc caattctggt
    39601 tggctccatc actgaccgct ttttctcggc gcaaaaagtg ctggcggtat tgatgttcgc
    39661 aggcgcgctg ctgatgtatt tcgctgcgca acagaccact tttgccgggt tctttccgtt
    39721 actgctggct tactctctaa cctatatgcc gaccattgcg ctgactaaca gcatcgcttt
    39781 tgccaacgtg ccggatgtgg agcgtgattt cccgcgcatt cgtgtgatgg gcactatcgg
    39841 ctggattgcc tccggtctgg catgtggttt cttgccgcaa atactggggt atgccgatat
    39901 ctcaccgact aacatcccgc tgctgattac cgccggaagt tctgctctgc tcggtgtgtt
    39961 tgcgtttttc ctgcccgaca cgccaccaaa aagcaccggc aaaatggata ttaaagtcat
    40021 gctcggcctg gatgcgctga tcctgctgcg cgataaaaac ttcctcgtct ttttcttctg
    40081 ttcattcctg tttgcgatgc cactggcgtt ctattacatc tttgccaacg gttatctgac
    40141 cgaagtcggc atgaaaaatg ccaccggctg gatgacgctc ggccagttct ctgaaatatt
    40201 ctttatgctg gcattaccgt ttttcactaa acgctttggt atcaaaaagg tattattgct
    40261 tggtctggtc accgctgcga tccgctatgg cttctttatt tacggtagtg cggatgaata
    40321 tttcacctac gcgttactgt tcctcggtat tttgcttcac ggcgtaagtt acgattttta
    40381 ctacgttacc gcttacatct atgtcgataa aaaagccccc gtgcatatgc gtaccgccgc
    40441 gcaggggctg atcacgctct gctgccaggg cttcggcagt ttgctcggct atcgtcttgg
    40501 cggtgtgatg atggaaaaga tgttcgctta tcaggaaccg gtaaacggac tgactttcaa
    40561 ctggtccggg atgtggactt ttggcgcggt gatgattgcc attatcgccg tgctgttcat
    40621 gatttttttc cgcgaatccg acaacgaaat tacggctatc aaggtcgatg atcgcgatat
    40681 tgcgttgaca caaggggaag ttaaatgaaa acagaacgta ttctcggtgc tctttatggg
    40741 caggcgttag gggatgcgat ggggatgccc tccgagcttt ggccacgcag ccgcgttaaa
    40801 gcacactttg gctggattga ccgttttctt cctggaccaa aggagaataa cgcggcctgt
    40861 tattttaacc gcgccgaatt caccgacgat acctcgatgg cgctgtgtct ggcggatgcg
    40921 ttactggaac gtgaaggcaa gatcgatccg gatctgattg ggcgtaatat tctcgactgg
    40981 gcgctgcgtt tcgacgcctt taacaaaaac gtactaggtc cgacctcgaa gattgcgctt
    41041 aacgccattc gcgacggtaa acccgttgct gaactggaaa acaacggcgt gaccaacggc
    41101 gcagcgatgc gcgtctcgcc attaggttgt ttgcttccgg cgcgtgatgt tgattccttt
    41161 attgatgatg tagcgctggc gtccagcccg acccataaat ccgatctggc ggttgcaggc
    41221 gcggtagtca tcgcatgggc gatttctcgt gccattgacg gagaaagctg gtcagcgatt
    41281 gttgattcac tgccttcaat tgcgcgacat gcccagcaaa aacgcaccac cacgttcagc
    41341 gcctcactgg cggcgcggct ggagattgcg ctgaaaattg tgcgcaatgc cgacggcacc
    41401 gaatccgcca gcgaacagct ttaccaggtc gttggcgcag gtaccagcac tattgagtcc
    41461 gttccgtgcg ccattgcgct ggttgaactg gcacaaaccg acccgaatcg ctgcgccgtc
    41521 ctgtgcgcta accttggcgg cgacacagac accatcggtg ctatggcgac ggcaatttgc
    41581 ggcgcgttgc atggcgttaa cgctatcgat cctgcgttaa aggcggaact ggatgcggta
    41641 aatcagcttg atttcaaccg ctatgccaca gcgttggcga aatatcgtca acaacgggag
    41701 gtggtatgag cggcgctcga ttacacacgc tgctgcctga attaaccacg cgtcagccgg
    41761 tgatggtcgt cggcgcggcg gtcattgacg tgatcgccga cgcttatgcc ctcccctggc
    41821 gtgggtgcga tatcgaactg aaacagcaga gcgttaacgt tggcggctgc gcactgaata
    41881 ttgccgtggc gttaaagcgc ctcggcatcg aagcgggtaa tgccttgccg ctcggtcagg
    41941 gcgtgtgggc ggagattatt cgcaaccgga tggcaaaaga gggcttaatc agcctgatcg
    42001 ataacgccga aggtgataac ggctggtgtc tggcgctggt tgagccggat ggcgaacgca
    42061 cttttatgtc attcagcggt gtggaaaatc agtggaatcg ccagtggctg gcgcgattaa
    42121 ccgttgcgcc tggcagcctg ctctattttt ccggttatca actggcctcg ccctgcggcg
    42181 aattgttagt ggaatggctg gaagagctgc aagacgtgac gccgtttatc gattttggcc
    42241 cacgtattgg cgatatcccg gatgcattac tggcgcggat catggcctgt cgacctttag
    42301 tgtcgctcaa tcgtcaagag gctgagattg ccgccgaacg ttttgcttta tccgcagaga
    42361 taacaacact tggcgagcaa tggcaggaga aatttgccgc gccgttgatc gttcgtctcg
    42421 ataaagaagg cgcatggtat ttcagcaacg acgcttctgg ctgcattccg gcatttccaa
    42481 cgcaagttgt agacaccatt ggggcgggcg acagtcatgc aggtggcgta cttgccgggc
    42541 tggcctctgg tctgccactg gcggatgccg tattactggg caatgcagtg gcgtcgtggg
    42601 ttgtcgggca tcggggcggt gattgtgcgc caacgcgcga ggaactactc ctcgcacaca
    42661 aaaacgtata gatcgctgcg acagtggcta atgctgtact caataggccg ttgctgttgg
    42721 tcaagcgcca cttgcttgat caccagcacc ggtattttgc tatctaactg aatatgtgac
    42781 tgaaactcgg catccggcat ccgggcgcta acgcgggaac gcgtacgttg tgggtaaatg
    42841 tgctggctgc ggaaatagtc ataaagtgaa atcccgatgg catcaacatc atgaattaaa
    42901 tgcgccggaa cccacgattc ctcaatcgat accgcttctt catcgacata gcgaatacgc
    42961 ttaagcaaaa agacatcact tcctgcttcg accgccagct gctgcgcgac ttcttcggga
    43021 catttcacga cacgcttgtt aacccatagc gtatcgggct ttttcccgcg caataccacc
    43081 tgctgagaaa aacctcgcgc ttctttcaac gagtattcga agatgttgtt gatctgcgtg
    43141 ccgtaaccgc gcgaacgcgt caccacaccc tcttcttcca gcgcctgcat cgccttgcgc
    43201 accgtaatgc gcgaaacgcc ggttaactga cttaaatcac gctcaccggg caaaatattg
    43261 ccatgctcca gcaccccgct gcgcacggca ttttttaccg tttcggcaaa cttaatataa
    43321 agcggcgtgt tatccgccgc taaaatacgc tcgttcagtt gggcaatcag ctgggtatgc
    43381 gcttgttcca tttgtctttt tcctgccgag gagtctggtg ggagtatatc actgcttctt
    43441 atttaaggcg tttctttaat cccatcaacg aatgcctgca actcctccac cgtgccatga
    43501 aatacattaa aatccaccgg accattgatg ccatctacct gtccacggtc ggaatgcggc
    43561 cagaagcgcc aggccatgcc gtcattgtcc ggacgacgtt gataatagtg agccacccac
    43621 cacggatatt cattgaaata gcccgccaga ttggtgtgat aaaaaacggc tcctgagtaa
    43681 ataatcggct tttttcccgt acttttttcg accattttta gccactgact tactcgtttg
    43741 cgtaattctt ttgccgataa ttttccccgt tcttctacgt ccagtaccgc agggaaatcg
    43801 ccttgtgaga aatccaccgt ttgcagaaat aatcgtgcct gaaccggcgc tgctaccgac
    43861 ggggagaaat aatgatacgc cccgcgcagc aggccatttt cgcggcttag ttgccagtta
    43921 cgcgaaaaat acgggtccac cagcttttcg ccttccgtcg ccttaataaa agcaaactgt
    43981 aagcggatac cgttgtcgcg catttttgcc acccgctgcc agtcgatccg ctcctgccag
    44041 cgcgaaacat ctattccgtg aatggtataa ctggcaggaa tgcgaattgc gaaagattta
    44101 accgggcgat aactgtagaa gtggatgtag tctgtaatct gaactgctgt cgaatagaaa
    44161 aaattcaccg tctgacgcgg ataaattgca acgatggaaa ttagcccaag agcgcataat
    44221 agagaggtga gtttttttcg actggtaatt cttagctgca ttggtttcat ccctgaatgt
    44281 cagtgccaaa ggctgacaat aaccaaagca gctatagtac ggtgctcacc accaggcgtg
    44341 gaagtggtga accggaccaa taccgtgacc aacttccagc gtgtcggcct gggctaatgc
    44401 tgatgaaagc cagctttttg cctcctgtac ggtgtcagcc cagtttgtat ggcgcgggcg
    44461 tagtgcagcc aacgccgcag agagcgtaca accagtgccg tgggtgtttt tggtcataat
    44521 gcgcggtgcg gtaaaccgtt gttcaccctc gcgggtaaac agccagtccg ggctttgctc
    44581 atcatccaga tgaccacctt tcattagcac tgcgccacag cccatcgcca acagcgatcg
    44641 cccttgttcc agcatttcct gttcggtgcg cgcgtgtggc gcgtcgagca aggcggcagc
    44701 ttcgggcaag tttggcgtta ttaatgaaac ctgtggcaat aatcgactgc gcagcgtagc
    44761 aaccgccgaa ggtgaaagca gcgggtcgcc gctttttgcc agcataacgg tgtcgagtac
    44821 cacgttttgg atctgataac gttgcaaccg ttctgccacc gcttcaacaa tatcggtttc
    44881 cgccaacata ccgattttag tggtgtcgat tcgcacatcg ctgaacaccg aatcgagctg
    44941 ggcggcgaca aaatcaggct caatgcgata caccgactgt acgccacggg tattttgcgc
    45001 caccagtgca gtaataactg agcaaccata agcgccaagt gccgagaagg ttttaagatc
    45061 ggcctgaatc cccgcaccac cactcggatc agtaccggca atcgtcagag cgttaattcg
    45121 tttcatgcct gcacctcctg cgtcaattgc cagagcgcat caaggaaatg tggaacaaaa
    45181 ctgcctggcc cctcgcttct ggcgactgcg cgctctccgg cttgtttcat ccagtgacag
    45241 gcagatgcga cattttccag cgtatcgcct ggtaacgcac agcaggtagc gacaaccgcc
    45301 gataatgcac agccagttcc taccactttg gtcattaacg gatcgccacc gtgaatacca
    45361 atgatacgat gtccatcggt aacataatcc atctcgccag tgaccacgac gattgcgcca
    45421 gtttcccgtg ccagtgtttg tgcagcgggt atcgcgttag ctgcggcgtc agtggtatcc
    45481 actccccgtc caccattagc aatgccagct aatgccatga tttccgaagc attaccacgt
    45541 atggcggttg gcttatgaga cagaagttcc agacaaaaac ggcggcgata atcgagtgca
    45601 ccaaccgcta ctggatcaag cgtccagggt gtttgagagc tttttgcttg ctcaacggcg
    45661 gcacacatcg actgtgcgcg tagctgcgtc agcgtgccaa cgttaatcag caacgcactg
    45721 gcaatagccg caaactgact ggcctcttcg gtttcgataa ccatcgctgg cgatgcaccg
    45781 agcgccagca aggtattggc agtaaaggtt tgcaccacat cgttggtcat gcagtgcaca
    45841 agaggggaat gttggtgaaa aaggtgtaac gcgtgcgcag attgcgctga actcagcagg
    45901 tcgacttgca tagtttgctc ctgccataac gtgaagaagc aatgacctgg tggtccgtga
    45961 cttccctacg ctggcattat ccagatcagg tgatacgggt atttctcagc cttcacgcag
    46021 aagggcaccc cgagtcgttt ggttgcgatg ataaatcgca gaggaggatg gtaatgtcca
    46081 gcgcacgcgt tgtaaacgag gaaaaacggc agttttacaa tcgcgttaat aaattatttg
    46141 atatatgaat ccagcacctt cagaacgaca tccagatctt cttcacgttt tagctcatcc
    46201 ccctggtgaa cgatgtgttc cgtcagatga cctttaatca cttcccgtat cagaccgttt
    46261 accgcgccac ggatagcagc aatctgttgt aaaactgcag cgcattcgtg cggctcgtcg
    46321 agcattttct tgagcgccac gacctggccc tgaatcttac tggcacgcgc tttcagtttc
    46381 tgtttatcac ggattgtatg agacatggca acacctggtt aacaagaata tgaaaaatca
    46441 tagcactatt aatctactgg ggggtagtat caggtactgg gggggagtag aatcagattg
    46501 ccgaattaat actaagaatt attatcatga ccgaatttac aactcttctt cagcaaggaa
    46561 acgcctggtt cttcatcccc agcgccatct tacttggtgc gcttcatggc ctggaaccag
    46621 ggcactcaaa aacgatgatg gcggcgttta tcatcgccat caaaggcacc attaaacagg
    46681 cggtgatgct cggactggca gcaactattt cgcataccgc agtggtctgg ttaattgcct
    46741 ttggcgggat ggtgatcagc aagcgcttta ctgctcaatc agcagaaccg tggctccagc
    46801 tgatttccgc agtgatcatt attggcaccg cgttctggat gttctggcgt acctggcgcg
    46861 gcgaacgcaa ctggctggag aatatgcacg agtatgatca tgaacatcat caccacgatc
    46921 acgaagatca ccacgaccat ggacatcatc accatcacga acatggcgag tatcaggatg
    46981 cccatgcacg agcccatgcc aatgatatta aacgacgctt tgatggtaga gaggtcacca
    47041 actggcaaat tttgttattt ggcttaacag gtggtcttat cccctgtccg gcagcaatta
    47101 ccgtgctgtt gatttgcatt cagttgaaag ccctgacact gggcgcaaca ctggtcgtca
    47161 gtttcagcat tggcctggcg ttaacgcttg tcaccgtagg cgttggcgca gcaatcagcg
    47221 ttcagcaggt cgcaaaacgc tggagcggat ttaacactct cgctaaacgc gccccctatt
    47281 tttccagtct gttgattggc ttagtcggtg tgtatatggg cgtacatggc ttcatgggca
    47341 taatgcgata actcaatcct ttaggcttgc atgttatctt cagcccagga tgattcttaa
    47401 atcagctatt tccgctgact ctctgctcgc taaggatgct tttagggcat cctttcattt
    47461 acacttttta cgaaatcatg ggatcactaa caaaatatcg cttgtcagtt atattgtatg
    47521 gcaggaaaga tatgcgactg atattacaga tccccaaagt ggagagttta tgaccattaa
    47581 aaataagatg ttgctgggtg cgcttttgct ggttaccagt gccgcctggg ccgcaccagc
    47641 caccgcgggt tcgaccaata cctcgggaat ttctaagtat gagttaagca gtttcattgc
    47701 tgactttaag catttcaaac caggcgatac cgtcccagag atgtaccgca ccgatgagta
    47761 caacattaag cagtggcagt tacgtaacct gcccgcgcct gatgccggga cgcactggac
    47821 ctatatgggt ggcgcgtacg tgttgatcag cgacaccgac ggtaaaatca ttaaagccta
    47881 cgacggtgag attttttatc atcgctaaaa aaagccccct catcatgagg gggaaatgca
    47941 gacaccttgt tattttttat tattagccac ttgctcgtct tgcttgttat tagtcgtatt
    48001 tcacgttgat taatgcggtt gcctccagtg cgccagattt aactttgttt gtatcgtaga
    48061 cgtagtaact ggcagttatc ggaattgaaa ccgttgtatc tgtaagtttt gactttgccg
    48121 caatttcttt taaatttgtg tatcctttac tgctgttagt gatatttaaa agtgtgactg
    48181 acgaaccatt actttcaaga ataaatccta cgccattatc ttccgcacct ttcagtattg
    48241 tgttgctgtt ggttaacaag tcagaacttg ataaatacac ctgcattttc cgtgatgttt
    48301 gattttgttg acctaataaa tttttacaac gcagaataat atccccttgt ttactattta
    48361 ttgttgcttt gttacctgtg gctggtaatt gtgaaacggg aatactgtct aaagtaatta
    48421 ataaatcgtc cggtttgcag gttgtggtta tcgggttgta tgacaatgtc agattaaagg
    48481 aatagacacc tgcattttgt gccaatgcat ttctgtagct atttacacat gcatcccatg
    48541 acttgccacc taataaacac agtagaaatt gtactgcggc actcctgcgt gtcgatgatg
    48601 aagcactgga taatgtcgca actccattaa tcatcactga accgccgccc cccgcagaaa
    48661 cattcacttt tgcattactg gccgcaggtg tcaccgttac cgaataacca ctggtgaaac
    48721 tggtctgatt atttgataac gtgatgttgt ttttattggt ccattc
//
LOCUS       DBANUU010000018        48490 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000018 DBANUU010000000
VERSION     DBANUU010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.018"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            165..1382
                     /gene="alaA"
                     /locus_tag="ACIE68_001006"
     CDS             165..1382
                     /gene="alaA"
                     /locus_tag="ACIE68_001006"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFX5907356.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1466..2065
                     /gene="yfbR"
                     /locus_tag="ACIE68_001007"
     CDS             1466..2065
                     /gene="yfbR"
                     /locus_tag="ACIE68_001007"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFX5907357.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     ATIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2124..3956)
                     /locus_tag="ACIE68_001008"
     CDS             complement(2124..3956)
                     /locus_tag="ACIE68_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFX5907358.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4043..4693)
                     /gene="hxpA"
                     /locus_tag="ACIE68_001009"
     CDS             complement(4043..4693)
                     /gene="hxpA"
                     /locus_tag="ACIE68_001009"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFX5907359.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLSHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4704..5198)
                     /locus_tag="ACIE68_001010"
     CDS             complement(4704..5198)
                     /locus_tag="ACIE68_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFX5907360.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5281..5736)
                     /gene="yfbV"
                     /locus_tag="ACIE68_001011"
     CDS             complement(5281..5736)
                     /gene="yfbV"
                     /locus_tag="ACIE68_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFX5907361.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6074..7276
                     /gene="ackA"
                     /locus_tag="ACIE68_001012"
     CDS             6074..7276
                     /gene="ackA"
                     /locus_tag="ACIE68_001012"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFX5907362.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7351..9495
                     /gene="pta"
                     /locus_tag="ACIE68_001013"
     CDS             7351..9495
                     /gene="pta"
                     /locus_tag="ACIE68_001013"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFX5907363.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9685..11205
                     /gene="yfcC"
                     /locus_tag="ACIE68_001014"
     CDS             9685..11205
                     /gene="yfcC"
                     /locus_tag="ACIE68_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFX5907364.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11238..11780)
                     /gene="yfcD"
                     /locus_tag="ACIE68_001015"
     CDS             complement(11238..11780)
                     /gene="yfcD"
                     /locus_tag="ACIE68_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFX5907365.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11838..12389)
                     /gene="yfcE"
                     /locus_tag="ACIE68_001016"
     CDS             complement(11838..12389)
                     /gene="yfcE"
                     /locus_tag="ACIE68_001016"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFX5907366.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12445..13089)
                     /gene="yfcF"
                     /locus_tag="ACIE68_001017"
     CDS             complement(12445..13089)
                     /gene="yfcF"
                     /locus_tag="ACIE68_001017"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFX5907367.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13225..13872
                     /gene="yfcG"
                     /locus_tag="ACIE68_001018"
     CDS             13225..13872
                     /gene="yfcG"
                     /locus_tag="ACIE68_001018"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFX5907368.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13929..14291
                     /gene="folX"
                     /locus_tag="ACIE68_001019"
     CDS             13929..14291
                     /gene="folX"
                     /locus_tag="ACIE68_001019"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFX5907369.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14312..15205
                     /locus_tag="ACIE68_001020"
     CDS             14312..15205
                     /locus_tag="ACIE68_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFX5907370.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15253..16143)
                     /gene="rpnB"
                     /locus_tag="ACIE68_001021"
     CDS             complement(15253..16143)
                     /gene="rpnB"
                     /locus_tag="ACIE68_001021"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFX5907371.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16340..17113)
                     /gene="hisP"
                     /locus_tag="ACIE68_001022"
     CDS             complement(16340..17113)
                     /gene="hisP"
                     /locus_tag="ACIE68_001022"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFX5907372.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVSGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17121..17837)
                     /locus_tag="ACIE68_001023"
     CDS             complement(17121..17837)
                     /locus_tag="ACIE68_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFX5907373.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17834..18520)
                     /gene="hisQ"
                     /locus_tag="ACIE68_001024"
     CDS             complement(17834..18520)
                     /gene="hisQ"
                     /locus_tag="ACIE68_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFX5907374.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18610..19392)
                     /gene="hisJ"
                     /locus_tag="ACIE68_001025"
     CDS             complement(18610..19392)
                     /gene="hisJ"
                     /locus_tag="ACIE68_001025"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFX5907375.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19613..20395)
                     /gene="argT"
                     /locus_tag="ACIE68_001026"
     CDS             complement(19613..20395)
                     /gene="argT"
                     /locus_tag="ACIE68_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFX5907376.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20661..21230)
                     /gene="ubiX"
                     /locus_tag="ACIE68_001027"
     CDS             complement(20661..21230)
                     /gene="ubiX"
                     /locus_tag="ACIE68_001027"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFX5907377.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21325..22842)
                     /gene="purF"
                     /locus_tag="ACIE68_001028"
     CDS             complement(21325..22842)
                     /gene="purF"
                     /locus_tag="ACIE68_001028"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFX5907378.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22879..23367)
                     /gene="cvpA"
                     /locus_tag="ACIE68_001029"
     CDS             complement(22879..23367)
                     /gene="cvpA"
                     /locus_tag="ACIE68_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFX5907379.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23626..24288)
                     /gene="dedD"
                     /locus_tag="ACIE68_001030"
     CDS             complement(23626..24288)
                     /gene="dedD"
                     /locus_tag="ACIE68_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFX5907380.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24278..25546)
                     /gene="folC"
                     /locus_tag="ACIE68_001031"
     CDS             complement(24278..25546)
                     /gene="folC"
                     /locus_tag="ACIE68_001031"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFX5907381.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25616..26530)
                     /gene="accD"
                     /locus_tag="ACIE68_001032"
     CDS             complement(25616..26530)
                     /gene="accD"
                     /locus_tag="ACIE68_001032"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFX5907382.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDHCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26686..27345)
                     /locus_tag="ACIE68_001033"
     CDS             complement(26686..27345)
                     /locus_tag="ACIE68_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFX5907383.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27428..28240)
                     /gene="truA"
                     /locus_tag="ACIE68_001034"
     CDS             complement(27428..28240)
                     /gene="truA"
                     /locus_tag="ACIE68_001034"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFX5907384.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28240..29253)
                     /locus_tag="ACIE68_001035"
     CDS             complement(28240..29253)
                     /locus_tag="ACIE68_001035"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFX5907385.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEACDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29319..30455)
                     /gene="pdxB"
                     /locus_tag="ACIE68_001036"
     CDS             complement(29319..30455)
                     /gene="pdxB"
                     /locus_tag="ACIE68_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFX5907386.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30554..31549
                     /gene="flk"
                     /locus_tag="ACIE68_001037"
     CDS             30554..31549
                     /gene="flk"
                     /locus_tag="ACIE68_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFX5907387.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31546..32724)
                     /locus_tag="ACIE68_001038"
     CDS             complement(31546..32724)
                     /locus_tag="ACIE68_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HFX5907388.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33008..34228)
                     /gene="fabB"
                     /locus_tag="ACIE68_001039"
     CDS             complement(33008..34228)
                     /gene="fabB"
                     /locus_tag="ACIE68_001039"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFX5907389.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34387..36393
                     /gene="mnmC"
                     /locus_tag="ACIE68_001040"
     CDS             34387..36393
                     /gene="mnmC"
                     /locus_tag="ACIE68_001040"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFX5907390.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTIPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36514..36792)
                     /locus_tag="ACIE68_001041"
     CDS             complement(36514..36792)
                     /locus_tag="ACIE68_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFX5907391.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(36826..37374)
                     /gene="epmC"
                     /locus_tag="ACIE68_001042"
     CDS             complement(36826..37374)
                     /gene="epmC"
                     /locus_tag="ACIE68_001042"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFX5907392.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37374..38183)
                     /locus_tag="ACIE68_001043"
     CDS             complement(37374..38183)
                     /locus_tag="ACIE68_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFX5907393.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38183..39007)
                     /gene="mepA"
                     /locus_tag="ACIE68_001044"
     CDS             complement(38183..39007)
                     /gene="mepA"
                     /locus_tag="ACIE68_001044"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFX5907394.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39011..40096)
                     /gene="aroC"
                     /locus_tag="ACIE68_001045"
     CDS             complement(39011..40096)
                     /gene="aroC"
                     /locus_tag="ACIE68_001045"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFX5907395.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40131..41063)
                     /gene="prmB"
                     /locus_tag="ACIE68_001046"
     CDS             complement(40131..41063)
                     /gene="prmB"
                     /locus_tag="ACIE68_001046"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFX5907396.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41229..41780
                     /gene="smrB"
                     /locus_tag="ACIE68_001047"
     CDS             41229..41780
                     /gene="smrB"
                     /locus_tag="ACIE68_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFX5907397.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(41902..42774)
                     /locus_tag="ACIE68_001048"
     CDS             complement(41902..42774)
                     /locus_tag="ACIE68_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFX5907398.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(42761..43285)
                     /locus_tag="ACIE68_001049"
     CDS             complement(42761..43285)
                     /locus_tag="ACIE68_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5907399.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(43282..43752)
                     /locus_tag="ACIE68_001050"
     CDS             complement(43282..43752)
                     /locus_tag="ACIE68_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5907400.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQVGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(43749..44297)
                     /locus_tag="ACIE68_001051"
     CDS             complement(43749..44297)
                     /locus_tag="ACIE68_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5907401.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(44272..45024)
                     /locus_tag="ACIE68_001052"
     CDS             complement(44272..45024)
                     /locus_tag="ACIE68_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFX5907402.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTPYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            complement(45044..47689)
                     /gene="yfcU"
                     /locus_tag="ACIE68_001053"
     CDS             complement(45044..47689)
                     /gene="yfcU"
                     /locus_tag="ACIE68_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFX5907403.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEEFSGDDTQKKWEWSRYYAWRALPSLKAKLALGEDYLNSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNMSVSLTGY
                     RYEYDNRADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDD
                     IKPVIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(47771..48334)
                     /locus_tag="ACIE68_001054"
     CDS             complement(47771..48334)
                     /locus_tag="ACIE68_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5907404.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        11994 a        13052 c        12007 g        11437 t
ORIGIN      
        1 ctcattattt tgtcattatt aagttattaa cagcacaatc gagccttccc ctctggcaaa
       61 atcttattct gcagaccttc aaaacaccgt cctgggggag tacattgttc taagctgact
      121 tccacggcag ggagtggcga taacagcaaa aaaggtcaag attcatgtcc cccattgaaa
      181 aatccagcaa attagagaat gtctgttatg acatccgtgg tccggtgctg aaagaagcaa
      241 aacgcctgga agaagaaggt aacaaggtac tgaaactgaa catcggcaac ccagccccgt
      301 tcggttttga cgcgccagat gaaatcctcg ttgacgtgat acgcaacctg cctaccgctc
      361 aagggtattg cgattccaaa ggtctttact ccgcgcgtaa agccatcatg cagcactacc
      421 aggctcgtgg catgcgtgat gttaccgtgg aagatattta catcggcaat ggtgtatcgg
      481 agcttatcgt tcaggcaatg caggcattgc tgaacagcgg ggacgaaatg ttggttcctg
      541 caccagatta cccactatgg accgcggcgg tttcgctttc cagcggtaaa gcggtgcatt
      601 atctttgcga tgaatcctct gactggttcc cggacctcga tgatattcgc gctaaaatta
      661 cgcctcgtac gcgtgggatc gttattatca acccaaataa cccaaccggc gcggtatatt
      721 ccaaagagct tttaatggag attgtggaga ttgcacgtca gcataatctc attatcttcg
      781 ccgatgaaat ttatgacaaa attctttacg acgacgctga gcatcactca attgcgccgc
      841 tggcacctga cctgctgacc attaccttta acggactgtc gaaaacgtac cgcgttgcag
      901 gcttccgtca ggggtggatg gtgttgaacg ggccgaaaaa acacgccaaa ggctacatcg
      961 aaggtctgga aatgctggct tcaatgcgcc tgtgtgctaa cgttcctgcg caacacgcca
     1021 ttcagaccgc gctgggtggt tatcagagca tcagtgaatt tattacccct ggcggtcgtc
     1081 tttatgagca gcgtaaccgc gcgtgggaac tgatcaacga tattccgggc gtttcctgcg
     1141 tgaaacctcg tggtgcgctg tatatgttcc cgaaaatcga cgccaaacgc tttaacattc
     1201 acgacgatca gaaaatggtg ctggatttcc tgttgcagga aaaagttctg ttggtgcaag
     1261 ggacggcatt caactggccg tggccggatc acttccgcat tgtcacgcta ccgcgtgtcg
     1321 atgatatcga gctgtctttg agcaagttcg cgcgtttcct ttctggttat catcagctgt
     1381 aatcttaatt tcactgccgg agattgcatc cggcagcgtt atcccgccac aatgacctga
     1441 tgatgtcatc atacgtaagg tcactatgaa acagagccat ttttttgccc atctctcccg
     1501 cctgaaactc attaaccgct ggccgctcat gcgcaacgtg cggacggaaa atgtgtccga
     1561 acacagtttg caggtagcga tggtcgccca tgcgctggca actatcaaaa atcggaaatt
     1621 tggcggtaat gtcaacgccg aacgtatcgc tttactggcg atgtaccacg atgcctcaga
     1681 agtgctcacc ggcgatctcc ctactccggt gaaatacttc aattcgcaaa tcgctcagga
     1741 gtacaaggct attgaaaaaa tcgctcagca aaaactggtc gatatggttc cggaagagct
     1801 gcgggatatc tttgcgccgt taattgacga gcatgcatat agcgatgaag aaaaatcgct
     1861 ggtgaaacag gcagatgcac tgtgtgcata tctgaaatgt ctggaagaac tcgcggccgg
     1921 aaataatgaa ttcttgctgg caaaaacgcg actggaagcg acgcttgaag cgcgtcgcag
     1981 ccaggagatg gactacttca tggaagtatt tgttcccagc ttccatcttt cgctcgatga
     2041 gattagccag gattcaccgc tgtaagcagc cggagtctgc gtcgcatcag gcaataagcg
     2101 ccggatgcga catcaggctc ttgtcaaaac ggaaacagca tcgggatcat caccacacaa
     2161 accgccatca cgataatggt gaacggtacc cccaacttca caaagtcact gaagctgtaa
     2221 tttcccggac ctaaaaccag tgtgttaaca ggtgaagaaa ccggtgtcat aaaggcggcg
     2281 gatgctgcca tcgccacgac catcgcgaat ggataaggcg acacccccat cgttttggca
     2341 gcagccagcg caatcggagc catcaacacc gccgtcgcgg tattagagat aaatagccca
     2401 ataaccgccg acaagacaaa caaacaaccc agcatcatat gtggcccgta accaccgcca
     2461 atgtccatca gccctttcac cgccagcgcg acacctcccg ttttctgtaa tgccacagca
     2521 aacggcatca tcccaacgat caaaataatg ctcggccagt gaatggattt ataggcgctt
     2581 tcagcatcta tacagcggaa tttccccatc agcaggcagg cgatgatagc ggcaacagga
     2641 ttaggaattt catctgtcag cattaacgcc accattagca ccagacagaa aatggcatgg
     2701 ggtgcctggc tgtgcgcggg cgatgcttca ctcacctctt ccggtaagtt cagcgctacg
     2761 aagtcgcggc cctgtttggc cagcataccg atcagtttcc agttacccac aaccaggatg
     2821 atatcgccca gcagcagagg ctcatccgcc agcgaacctt ccagcgccac gccattgcgc
     2881 tttagcccca ccacattcag tccgtagcgg gtacgaaaac caatttcgcg caccgattta
     2941 ccaatcagtt ctgactcagg aattaatgaa atctctgcca tgcccacatc aagggcctgg
     3001 tcagaaaaat actcgccgcg cagtaccatc ggctccagca attgctcact acaaaattgc
     3061 cggagatcga catcagccgc agacatatca ataagcaaaa cgtcacgcgc gcgaaattca
     3121 gaaaccccat taacgttcac gataacgcga cgaaaacgcc gccagcgttc aacaccgatg
     3181 acgttagcgc cataacgctc acgtaatttg agatcatcca gccgttgacc tatcattggc
     3241 gatccggggc gaatagccag acgtcgcgca cgcccggtca gtcgatattc acggataaga
     3301 tcgcgaaagg ttcgacgcgt ccagccttcg cgctgcgggg tctgggtatc ccctttcagc
     3361 atgaaacgca tcactaacat atacaaaata cccagcacca gcacgaccag gccaataggt
     3421 gttacgctaa agaaactgaa gccgtgatag ccttcacgca gcaattcact gttgactacc
     3481 aggttcggcg gcgtcgccac cagcgtcatc atgccgctta tcagcccggc aaaactcaac
     3541 ggcatcatca gacgtgacgg cgaagtttgc atacgcatgg caacgcttaa caccacggga
     3601 ataaagatag cgacaacgcc ggttgaactc ataaacgcgc caagccccgc gacggtcagc
     3661 atcaacaaaa ccaacatttt gatttcactg ttgcccgcaa ctttgaccag ccatgttccc
     3721 attacggtgg caacaccggt acggaccaaa ccatcgccaa taataaacaa ggcggcaatc
     3781 aggacaacgt taggatcaga aaagccggaa aatacttcgg ggactgtcag cgttccgctt
     3841 aatgcaaacg cgacaataac aaacaaagcg accgcatcca tacgcactct gcccgtcgca
     3901 aacaagacaa tggcaacggc cagtaatgaa agaacccaaa tcaattcacc gttcacaact
     3961 tatccttgtt aattgaggga gatgtcttga ttctgccata aaaaagcccc gacgagacgg
     4021 ggctaaatca tgatcaggtg tttcactgaa taataacatc gccatttggc tgtttggtca
     4081 cagtaatttg ctccagactg tggaggacca aatcgacctc attcaggcgc ggggtatctg
     4141 ccggagcgtt aaccgcaatg acatggcaac ccgccgccag gccagaaagc acgccagcgg
     4201 gagcatcttc caccaccaca cactcctgcg gcgcaagccc cagcagctgc gcacctaaca
     4261 gatacgcatc aggttctggt tttccgcgct tcactcgctc agcggttaca aacacctctg
     4321 gtgcgggaag cccagctatt ttatggcgcg ctcgcgctac cggcatggag ccagaagtca
     4381 caatggccca cggaatacct gctttattca aatgactgag taaggcgatt gcccccggaa
     4441 gcgcggtaat accttcggtt tccgtggcct cgatttgctc cagacgcgta aactcggcgg
     4501 caatatcagc ctcggatttg cccgccataa aatggcgcag agaggtgatc gcctgtttac
     4561 cgtgaatgaa agccagcacc tcttccggcg ctaacccatg acgtctggcc cagttgctcc
     4621 acgcccgttc taccgcaggc agggaatcca ccagcgttcc atcaagatca aacagaaaac
     4681 ctttgcaccg cacgcgggcc tcctcaggcg ttgataattt gattaatttc gttggcgctc
     4741 aaatggtact gacgcgggca ggcatgccac acattaagca tgcgctgata tttttcccac
     4801 attggcgtct gggcgttaaa accgtgagtt ccggcgtcaa aatgggtata gcgcccttcc
     4861 acattaacca taaagcggac ataaccgagg taacgtgctt cagtggcggc gtcaaagccg
     4921 aggaaggtga cacgacgttc atcgatggat tgctggtcct gcaaattaga ccaggaaaca
     4981 tgcaacgcat gatacatctc cataatgtcg atgatagtgc ggcaggtttc ttctttcagc
     5041 tcgccaaact cgcgatccaa ttcacgcatc tgtaatccgt aaccacgctc aataattgtt
     5101 tgcaggcgac ggtaacgttc agcatttgcc ggatcgagca tagtcatcat cttgtactga
     5161 ttagacaaaa taagacgttg cgcgttggtc atttccattg ttgactcctg tatcactata
     5221 ctacggtgaa aaaaaagaag gctgagtatg ccttctttta tatgcgtaat caggggtcaa
     5281 ttacaaatca tcaaggaaag ttttatccag ttgtttgaag gcgcgcttaa gcgtgtcagc
     5341 taatgcctgg taatcaggct tgccttcaac gggtgccaac acctgtccag actcctgcaa
     5401 tttaccgcga acttcataaa accagttaag gattgcaggg ggtaatggcg ttacagaacg
     5461 cttgcccagc caccacaatc cctgcatggg taaacttaag gcgaacagcg cagtggcaac
     5521 tgccggccca agctgaccgc ccagggcaat ctgccagcag agagtaaata cggcgatcgg
     5581 cggcataaaa cggatcgcat aacgcgtcat cttgataacg cgattttcga caaagaccgg
     5641 ggcaaggcgt ttttccagtg gccacgtctt tgagtaatgc tgtccgcggc gaaacaagct
     5701 aaaaaaatta acagaacgat tatccggcgt tgacatgctt cacctcaact tcacatataa
     5761 agattcaaaa atttgtgcaa attcacaact cagcgggaca acgttcaaaa cattttgtct
     5821 tccataccca ctatcaggta tcctttagca gcctgaaggc ctaagtagta catattcatt
     5881 gagtcgtcaa attcatatac attatgccat tggctgaaaa ttacgcaaaa tggcatagac
     5941 tcaagatatt tcttccatca tgcaaaaaaa atttgcagtg catgatgtta atcataaatg
     6001 tcggtgtcat catgcgctac gctctatggc tcactgacgt ttttttagcc acgtatcaat
     6061 tataggtact tccatgtcga gtaagttagt actggttctg aactgcggta gttcttcact
     6121 gaaatttgcc atcatcgatg cagtaaatgg tgaagagtac ctttctggtt tagccgaatg
     6181 tttccacctg cccgaagcac gtatcaaatg gaaaatggac ggtaataaac aggaagcggc
     6241 tttaggtgca ggcgccgctc acagcgaagc gctcaacttt atcgttaata ctattctggc
     6301 acaaaaacca gaactgtctg cgcagctgac tgctatcggt caccgtatcg tacacggcgg
     6361 cgaaaagtat accagctccg tagtgatcga tgagtctgtt attcagggta tcaaagatgc
     6421 agcttctttt gcaccgctgc acaacccggc tcacctgatc ggtatcgaag aagctctgaa
     6481 atctttccca cagctgaaag acaaaaacgt tgctgtattt gacaccgcgt tccaccagac
     6541 tatgccggaa gagtcttacc tctacgccct gccttacaac ctgtacaaag agcacggcat
     6601 ccgtcgttac ggcgcgcacg gcaccagcca cttctatgta acccaggaag cggcaaaaat
     6661 gctgaacaaa ccggtagaag aactgaacat catcacctgc cacctgggca acggtggttc
     6721 cgtttctgct atccgcaacg gtaaatgcgt tgacacctct atgggcctga ccccgctgga
     6781 aggtctggtc atgggtaccc gttctggtga tatcgatccg gcgatcatct tccacctgca
     6841 cgacaccctg ggcatgagcg ttgacgcaat caacaaactg ctgaccaaag agtctggcct
     6901 gctgggtctg accgaagtga ccagcgactg ccgctatgtt gaagacaact acgcgacgaa
     6961 agaagacgcg aagcgcgcaa tggacgttta ctgccaccgc ctggcgaaat acatcggtgc
     7021 ctacactgcg ctgatggatg gtcgtctgga cgctgttgta ttcactggtg gtatcggtga
     7081 aaatgccgcg atggttcgtg aactgtctct gggcaaactg ggcgtgctgg gctttgaagt
     7141 tgatcatgaa cgcaacctgg ctgcacgttt cggcaaatct ggtttcatca acaaagaagg
     7201 tacccgtcct gcggtggtta tcccaaccaa cgaagaactg gttatcgcgc aagacgcgag
     7261 ccgcctgact gcctgatttc acaccgccag ctcagctggc ggtgctgttt tgtaacccgc
     7321 caaatcggcg gtaacgaaag aggataaacc gtgtcccgta ttattatgct gatccctacc
     7381 ggaaccagcg tcggtctgac cagcgtcagc cttggcgtga tccgtgcaat ggaacgcaaa
     7441 ggcgttcgtc tgagcgtttt caaacctatc gctcagccgc gtaccggtgg cgatgcgccc
     7501 gatcagacta cgactatcgt gcgtgcgaac tcttccacca cgacggccgc tgaaccgctg
     7561 aaaatgagct acgttgaagg tctgctttcc agcaatcaga aagatgtgct gatggaagag
     7621 atcgtcgcaa actaccacgc taacaccaaa gacgctgaag tcgttctggt tgaaggtctg
     7681 gtcccgacac gtaagcacca gtttgcccag tctctgaact acgaaatcgc taaaacgctg
     7741 aatgcggaaa tcgtcttcgt tatgtctcag ggcactgaca ccccggaaca gctgaaagag
     7801 cgtatcgaac tgacccgcaa cagcttcggc ggtgccaaaa acaccaacat caccggcgtt
     7861 atcgttaaca aactgaacgc accggttgat gaacagggtc gtactcgccc ggatctgtcc
     7921 gagattttcg acgactcttc caaagctaaa gtaaacaatg ttgatccggc gaagctgcaa
     7981 gaatccagcc cgctgccggt tctcggcgct gtgccgtgga gctttgacct gatcgcgact
     8041 cgtgcgatcg atatggctcg ccacctgaat gcgaccatca tcaacgaagg cgacatcaat
     8101 actcgccgcg ttaaatccgt cactttctgc gcacgcagca ttccgcacat gctggagcac
     8161 ttccgtgccg gttctctgct ggtgacttcc gcagaccgtc ctgacgtgct ggtggccgct
     8221 tgcctggcag ccatgaacgg cgtagaaatc ggtgccctgc tgctgactgg cggctacgaa
     8281 atggacgcgc gcatttctaa actgtgcgaa cgtgctttcg ctaccggcct gccggtattt
     8341 atggtgaaca ccaacacctg gcagacctct ctgagcctgc agagcttcaa cctggaagtt
     8401 ccggttgacg atcacgaacg tatcgagaaa gttcaggaat acgttgctaa ctacatcaac
     8461 gctgactgga tcgaatctct gactgccact tctgagcgca gccgtcgtct gtctccgcct
     8521 gcgttccgtt atcagctgac tgaacttgcg cgcaaagcgg gcaaacgtat cgtactgccg
     8581 gaaggtgacg aaccgcgtac cgttaaagca gccgctatct gtgctgaacg tggtatcgca
     8641 acttgcgtac tgctgggtaa tccggcagag atcaaccgtg ttgcagcgtc tcagggtgta
     8701 gaactgggtg cagggattga aatcgttgat ccagaagtgg ttcgcgaaag ctatgttggt
     8761 cgtctggtcg aactgcgtaa gaacaaaggc atgaccgaaa ccgttgcccg cgaacagctg
     8821 gaagacaacg tggtgctcgg tacgctgatg ctggaacagg atgaagttga tggtctggtt
     8881 tccggtgctg ttcacactac cgcaaacacc atccgtccgc cgctgcagct gatcaaaact
     8941 gcaccgggca gctccctggt atcttccgtg ttcttcatgc tgctgccgga acaggtttac
     9001 gtttacggtg actgtgcgat caacccggat ccgaccgctg aacagctggc agaaatcgcg
     9061 attcagtccg ctgattccgc tgcggccttc ggtatcgaac cgcgcgttgc tatgctctcc
     9121 tactccaccg gtacttctgg tgcaggtagc gacgtagaaa aagttcgcga agcaactcgt
     9181 ctggcgcagg aaaaacgtcc tgacctgatg atcgacggtc cgctgcagta cgacgctgcg
     9241 gtaatggctg acgttgcgaa atccaaagcg ccgaactctc cggttgcagg tcgcgctacc
     9301 gtgttcatct tcccggatct gaacaccggt aacaccacct acaaagcggt acagcgttct
     9361 gccgacctga tctccatcgg gccgatgctg cagggtatgc gcaagccggt taacgacctg
     9421 tcccgtggcg cactggttga cgatatcgtc tacaccatcg cgctgactgc gattcagtct
     9481 gcacagcagc agtaatctcg tcatcatccg cagctttgcg ctgcggatat ctgaaccgga
     9541 aataatcact atttccggtt ttttattctc ttaatctgca ttaatccttt ctgattatct
     9601 tgcttaactg cgctgcatca atgaattgcg ccatttcact ttgcatactt accactttgt
     9661 tttgtgcaag ggaatatttg cgctatgtcc gcaatcactg aatccaaacc aacaagaaga
     9721 tgggcaatgc ccgatacgtt ggtgattatc ttttttgttg ctattttaac cagccttgcc
     9781 acctgggtag ttccggtggg gatgtttgac agtcaggaag tgcagtatca ggttgatggt
     9841 caaacaaaaa cacgcaaagt cgtagatcca cactcatttc gcattctgac taacgaagca
     9901 ggcgaacctg agtatcaccg cgtacagctg ttcacgacgg gcgatgaacg cccgggcctg
     9961 atgaacttcc cgtttgaagg attaacctca ggatcgaaat acgggacagc tgttggcatc
    10021 atcatgttta tgctggtgat tggcggcgcg tttggcattg tgatgcgtac aggaaccatt
    10081 gataacggta tcctggcgct tattcgccat acccgcggga atgaaattct ctttattcct
    10141 gcgctgttta ttctgttttc acttggcggc gcggtatttg gtatgggaga agaggccgtc
    10201 gcctttgcca ttatcatcgc accgctaatg gtccggctgg gctatgacag tattaccacc
    10261 gtcctggtga cctatattgc cacgcaaatc ggttttgcca gttcgtggat gaacccgttt
    10321 tgtgtggtcg ttgctcaggg gattgccggc gttccggtgc tttctggctc cgggttgcgc
    10381 atcgtggtgt gggttatcgc cactctgatt ggcctgatct ttaccatggt gtacgcctca
    10441 cgagtgaaaa agaatcctct tctgtcacgc gtgcatgagt ccgaccgctt ctttcgtgaa
    10501 aagcaggctg atgttgaaca acgtccgttt acctttggtg actggctggt attgattgtc
    10561 ctgaccgccg taatggtctg ggtgatttgg ggcgtgatcg ttaatgcctg gtttattcca
    10621 gaaattgcca gccagttctt caccatgggt ctggtgattg gcatcatcgg cgttgttttc
    10681 cgccttaacg gcatgacggt taataccatg gcttcatcct ttaccgaagg ggcgcgaatg
    10741 atgatcgccc ctgccctgct ggtgggtttc gccaaaggga ttttgctgct ggtcggtaat
    10801 ggtgaagcag gtgatgccag cgtgttaaat accatcctca acagcattgc caatgccatt
    10861 agcggtctgg acaacgctgt cgcggcctgg tttatgttgc tcttccaggc ggtatttaat
    10921 ttctttgtga catccggttc tggtcaggcg gcgttaacca tgccgttact ggcaccgctt
    10981 ggcgatctgg tcggtgttaa ccgtcaggtt accgtgctgg cattccagtt tggtgatggc
    11041 ttcagccaca tcatttaccc aacctcagct tcgttaatgg cgacgctcgg tgtttgccgg
    11101 gtggacttcc gtaactggct gaaggtgggc gcgacactgc ttggactgct gtttattatg
    11161 tccagcgtcg tggtgatcgg cgctcagttg atgggctacc actaaaaatg ttaagagccg
    11221 caattgcggc tcttttttca ttctgccgtt tcagtctcta cggcttcatt tttggcattg
    11281 cgcttcatcc acaacgccag cgctttcagc gagtctggag tgaactcatc gcagcgtgcg
    11341 gtgatttctt ccggcgtcag ccagcaaact tcactgactt catcttcctg tagggcgaag
    11401 ggaccgtgag agacgcagct gaacaatgcg ccccagacac ggcaattttt atcttcgaaa
    11461 tagaactgcc cgtgctcagc aaaggggaca ccggcaatgc ccaactcttc ttccgcttcg
    11521 cgacgcgcgg attccagcag ttgctcatcg gcctggacca caccgcctgc ggtcgcatct
    11581 aacatgccgg gtaaaaagtc ttttgtctcg gtacgacgct ggaccagaat tttgcccatg
    11641 ccatcatgca cgacgatgta agttgcacga tgacgcagac actgtgcccg catttgttcc
    11701 cggctggctt gtgcaatgac ttcgttctct tcattgacaa tatccaccca ttcagtactt
    11761 gccaaacgac gctgttccat tatcaggaaa ccttcttttt ctggcgctct tacggcgcat
    11821 tttgagttgt gggtaaatta cggattaatc gcgacctgcg caatgatact ttgatcattg
    11881 agtgcgatta cactaagtac gtcattatcc agcatgccat aactcgccgg attaccgcct
    11941 ttcggaatac tcaccgagcc ggggttgaag tggaaaatct ccccccgctg ttctgccact
    12001 ggcagatggg tatgaccgta caccagcaca tcgttctggt ttaaagcagg tagattttcc
    12061 gggccaaaaa gatgaccatg cgtcaaaaac agacgttgtt tttccagtaa tacctgttgc
    12121 cacggcgcgg ttatcgggaa atgcagcagc atttgatcca cttcgctgtc gcagttgccg
    12181 cgcacagcga taaccttatg tgccacttca ttaagccgtt cagcgacttt ggctggcgcg
    12241 taaccctccg gtaaagcatt acgcgggccg tgattcaaca cgtcgccaag gatcaccagc
    12301 cactgggcac cgctttgggc aaacaactcc agaacacgtt ccgtcgccgg taacgaccca
    12361 tgaatgtccg atgcaaacat cagtttcatc actcactcct cgtcgaaaaa acgtaagcta
    12421 tgatactgga ttctgtcgtt gctatcagcc agattgcttc gccgaaagtg caataaaacg
    12481 ctggacagac gctcgctgcc actggaatgt cgcataatcc accaggcgtt ctggcacctc
    12541 atcgccatgt agtaccaggc ggttaatcat tagcgccaga tcagtatcag caatgcacca
    12601 ttcaccaaat aaattcggct gacccagtgc taacaaatgt tctgccatcg cgaacagttt
    12661 ctctgcactg gcttttccct cggccgttag tggcgctttt ttcgcccccg caaagacaac
    12721 atccgtcgga cgctcttcgc ggatgggcat cagatcgctg cgcagccagg cctgaatctg
    12781 tcgtgcacgc gcacgatttt ctaaatcaag cggataaata cgttcccagg tcggtggcgc
    12841 aaatcgatct tccagatact ccgcaatggc agaagattca ctcaactcaa aatcatcgat
    12901 ttgtaataac ggcacacggc gtgtctgacc gtaaccttgc cacgtcggct gcaaatgttc
    12961 accgctgtcg aggtcgatgg tcttgatatg aaacgacagg cctttttcct gcaacgccac
    13021 ccaggcggat aacacataag gggagaaaaa gtgggcatct gaccaaagcg tgatagcggg
    13081 tttactcatt atgtcctcgg caataaaggg ggctggctat caaactatag agcctttttg
    13141 tcactgtcac gtgacgaata ctcacgtaag tcgccgcgct ttctatactt gtggtgttca
    13201 gatgattaaa caaccggagc tgcaatgatc gatctctatt tcgccccgac acccaatggt
    13261 cacaaaatta cgctgtttct cgaagaagca gagcttgatt atcgcttgat taaggtagac
    13321 ctggggaaag gcggtcagtt tcgtccggaa tttttgcgca tttcgcccaa caacaaaatt
    13381 ccggcaattg ttgatcattc tcctgccgat ggcggcgaac cgttaagcct ctttgaggct
    13441 ggtgccattt tgttgtatct ggctgagaaa acaggactct ttttgagtca tgaaacgcgt
    13501 gagcgcgccg ccacattaca gtggttattc tggcaggtag gcggactggg gccgatgctt
    13561 gggcaaaatc atcattttaa tcacgcagcc ccccaaacca ttccttacgc tattgaacgt
    13621 tatcaggttg aaactcagcg tctttaccat gtactgaaca agcggctgga aaactcgccc
    13681 tggctgggag gcgagaacta cagcattgcg gatattgcct gctggccgtg ggttaatgcc
    13741 tggactcgcc agcgaattga cctcgcaatg tatccggcag tcaagaactg gcatgagcgg
    13801 atccgttcgc gccctgccac cgggcaggca ctgctaaaag cacaactcgg tgatgagcgt
    13861 tcggatagtt aacagaaaca ggttctcgtg tattatttca tcctaagtaa aacaacggag
    13921 aacctgcaat ggcacaacct gccgctatta ttcgtataaa gaaccttcgt ttacgtacgt
    13981 ttatcggaat taaggaagaa gaaattaaca accgtcagga tattgttatc aatgtgacga
    14041 tccactaccc cgccgataaa gcgcgcacta gcgaagatat caacgatgcg ctgaattatc
    14101 gcaccgtaac gaaaaacatt attcagcatg tagagaataa ccgtttctct ttgttggaaa
    14161 aattaactca ggatgtgctc gatatcgcac gtgaacatca ctgggtgacg tatgctgaag
    14221 tggagatcga taaactgcac gcgctgcgct acgccgattc ggtatccatg accttaagct
    14281 ggcagcgtta accgccatat cgggaggctg catgaacata gtgatcaccg gagggacggg
    14341 attaattggt cgccatttga ttccgcgttt gctggagctg ggccatcaaa tcacggtagt
    14401 gacgcgtaac ccacagaaag ccagttccgt tctcggccct cgggtgacgc tatggcaagg
    14461 gctagccgat caaagcaacc tcaacggcgt tgatgcggta atcaacctgg ccggagaacc
    14521 gattgctgat aaacgctgga ctcacgagca aaaagagcgt ctctgccaaa gccgctggaa
    14581 tatcacgcaa aaactggtcg atttgattaa tgccagcgac acgccgccgt cggtactcat
    14641 ttccggctcg gcaacgggct attatggcga cttaggtgaa gtggtggtta ccgaagagga
    14701 accgccgcat aacgaattta ctcataaact ctgcgcccgc tgggaagaga ttgcctgccg
    14761 ggcgcaaagt gacaaaacgc gagtgtgcct gctgcgaacc ggcgtggtac tggcaccgga
    14821 tggcggtatt ctcggtaaaa tgctgccgcc gtttcgtctt ggcctgggcg ggccgattgg
    14881 ctctggtcgg cagtatctgg cctggattca tattgatgat atggtcaacg gtattctctg
    14941 gctgctggat aacgagctgc gcgggccatt taatatggtt tcgccctacc cggtacgcaa
    15001 tgaacaattt gcccatgcgc tcggtcatgc gctgcatcgc ccggccattt tgcgcgtccc
    15061 cgcgaccgcc attcgactgt taatgggcga atcttcagta ctggtattag gtggacaacg
    15121 cgcgctgcct aaacggctgg aagaagcggg ttttgcgttt cgctggtacg atttagaaga
    15181 ggcgctggcg gatgtcgttc gctgatgtgg tttacagcaa acatccgcca gttaactccc
    15241 ggtgttacag gattagtggc tttgcgctat aagatcgtct ggcgatagtc gggtcaccat
    15301 cataactaac tctctgtcta aacctctatc cagcatctcc tgagcaatac gcagggcttc
    15361 ttcgtgtttg ccctgcattg cgccttcttc acgtaatctg tcagcaatgg tcatcagttt
    15421 ctccttttct tgtggtgcgc gttctgttat ctcaccaata aatgcacgga aacgctgggc
    15481 atcccctgtt tgtaatacgt aattaaacag ggcttttagc tgtctgtcat tagtgttccc
    15541 tgtaactagc agcgaaacaa tttggtcgac taatcccaac agatcgcgct gacgaatatg
    15601 tttctgaatt aactccaaca gcgccatttt gcggtgttgc ataatctcgt catccggcac
    15661 cacggtaata tccaccaacg gaaacgctga tgaatatatt ttgcgggcta tagcaggctc
    15721 ggcaaattca tcaagccagc agagtgaata aggataagga cttctgcaac catgataaaa
    15781 cagcatcggg atcaccaatg gaagctcttt atagcccgca tcaagatggt tttgcattgc
    15841 cgcaatggaa taacgcatca tgcgaaaagc cattaattct tccggcttac tttggtgctc
    15901 tatcactaca taaatataac ccactccctc ctgcgttttc acagaccaca agaggtcgga
    15961 ataatattgc cgcaggtcgt catcaataaa actgtttggt tccagtttaa gcgtcgttaa
    16021 atcacacagt ttgcgcagcg gcgcgggaag atgaatatca ataaaatccc gcgcggtgtc
    16081 tggatggcgt aaaaaagatt taaataccgc atcatgcggc gtggaagttg tcgatattgt
    16141 catggcgttc cgtcacccct caaatttaag atgacgcgac aataaccacg attaacaccc
    16201 aatgcagcag gcagttttta tctttggaca gcgaattcag aagtattttt agcggactgc
    16261 aacttttaca tttcagcgga agctgactcg caaattcccc atgaaaatac tccggcagcg
    16321 ttagccaccg gagaggaaat tatttcagcg atcccttaag gaactgttgc agacgagggc
    16381 tttgcgggtt gccaaataac tgctccggcg cgccctcttc ttctattttc ccctgatgga
    16441 ggaaaatgac gtgagtagaa acatggcgag caaagcccat ttcgtgagtc actaccacca
    16501 tggttttccc ctcttctgcc agttgctgca taatacgtaa cacttcgcct accagttcag
    16561 gatcgagcgc cgaggtaggt tcatcaaaca gtaaaacttc cggttccatc gccagcgccc
    16621 gcgcgataga aacacgctgt tgctgaccgc cggaaagatg caccggatat ttcccctgcg
    16681 cacgttcgtc tatcccgact ttcgccagat acttcaccgc ccgctcgcgc gcttcctgct
    16741 tgctcaggcc caacacctga atcggcgctt ccatgacgtt ttccagcacc gtcatatggc
    16801 tccagagatt gaagtgctgg aataccatcg tcaggcgtgt gcgcagtaag cgcagttgat
    16861 ttttatcggc gactttgagt tgaccatctt tgtcgcgcac cagattgatc gtctggccac
    16921 tgaccacgat cgatccttcg ctcggttttt cgaggaagtt aatgcagcgc agaaaggtac
    16981 ttttccccga tcccgacgat ccgatgatgc ttattacatc tccggcattc gcttgcagtg
    17041 acaccccttt cagcacttcg tgttcgccat agcgtttgtg caaatcgata acgtttaatt
    17101 tattctcgga catcgtattc tcagtgcgtt gaagaaggtt tcacatgctg caaccagcgt
    17161 ttttccgctc tgcgaaagag gctgatcagg acataagaga tgattaaata gagtaccgcg
    17221 gcaatgccga aggcggtaaa aggttgatac gtggcggcgt taatatcgcg ggctattttc
    17281 agcagatccg gcaccgtggc cgtaaacgcc agcgcagtag agtgcagcat caggatcact
    17341 tcattgctgt atgccggtaa cgcaatacgc agcgcagaag gcaaaataat gcagcgatac
    17401 attttaaagg tcgagaagcc ataggctctg gcggcttcaa tttccccatg cggaaccgaa
    17461 cggattgccc cagcaaaaat ctcggtagtg taagcgcagg tgttaagcgt cagcgccagc
    17521 acggtacagt tcaggccact gcggaagaaa gcgttaagga attcggttcc cttaacaatc
    17581 tcaagcgtgt acatgccgga atagaacacc agcaactgaa catacagcgg cgtaccgcga
    17641 aaaatatagg taaataacca gattggaaac tggatgtatt tattactgga gacacgacca
    17701 atcgccagaa acagcgccag gactccgcct atcactaccg acaaaataag cagccacaga
    17761 gtgatcgcca caccagtaaa gcgataaccg tcggtccaca gcagcggttt ccagtattca
    17821 tgtaagattt cgatcacagg tcagccctct tcacacccac ggagtagcgg cgctcaagga
    17881 acagcagcac accattggaa acagtggtga aaaccaggta aatcacgcca cagacgatgg
    17941 cgaaatagaa cggttcccag gtacttttgc ctgccagttg ggtggctttg accacatctt
    18001 ccaggccgag taacgaaacc agtgcggtag atttgaggat cacctgccag ttgttgccaa
    18061 tgcctggcag ggcgtaacgc atcatcgccg gaaacatgat ccgccgaaac acttgcccac
    18121 gagtaaaacc gaacgccgtc gccgcctcta tatgtccttt cggcactgcc ataaaagcac
    18181 cacggaacgt ttcggtaaaa taagcaccgt agataaaacc gagagtgata ataccagcga
    18241 ccatcggatc gatatcaatc tgcccgacgc ccatcgcctc cgtcaccgtg tttagcgcaa
    18301 tctgcaaacc gtagaaaatc agcagcatca acactaagtc cggcacgcca cgaatcagcg
    18361 tggtgtaccc ttcgaaaatc agccccgaaa gccgattttg cgagagctta ccgccagcgc
    18421 caattaaacc gatgattaca gcgagcacta cagagctgat agccagctcc agcgtgacga
    18481 gcgcaccctg taaaataaca cctgaaaacc catacaacat gctgcctgtc ctgtcgtgtg
    18541 tggtgaatta ccgcctcttg tctccctccg gttacccgaa gggagcgagc tttctttcac
    18601 cgatggcgat taaccgccat aaacatcaaa atcgaagtac tttttcgcta atttctcgta
    18661 agtaccgtca gcgcgcattt cggcaaaggc tttgttcagt gcctcgcgca gttcgttatc
    18721 ttctttacgc aggcccatgc cggtccctac accaaacagt ttttcatctt taacagacgg
    18781 gccaccgaat ttgtaatctt taccgacggg ttgtttgagg aaaccttcac tggcagcgac
    18841 ctcatcctgg aacgcggcat caatacgtcc ggcagtcagg tcagaataaa tgttgtcctg
    18901 cccctgatac gagacgattt caatgccttt tggtgcccag tgttcgttac cgaacgtctc
    18961 ctgggtggtg ccctgcaata cgcctacccg cttgcctttc agcgactcga ctgtcggctg
    19021 aatgtcggaa tttttcgcca ccaccagacg agaatcggca gcgtacagtt tgtcggtgaa
    19081 ggcaatttct tgctgacgtt tttccgtaat ggaaagcgat gacatgatgg cgtcaatctt
    19141 cttcgctttt aaggacggga ttaacgcatc cagcggattt tcgacaaacg tacattgcgt
    19201 attgatgcgt ttgcataatt cctttgccag atcgatatcg aagccaacca gttcgccttg
    19261 tgaatttttt gattcaaatg gcgcataggt cgggtcggta ccgatgcgga tgttttgcgg
    19321 aatcgcagca aatgccgcag ttgcgctgga gaaggccaga accagagaga gcgatagcac
    19381 cagttttttc ataatgtcct caactgacag tctttttata gggaatattt acaggtttgt
    19441 tacacttatc gtgccataaa attgaccatc aaggcaaaat atcccgcccc gttagacgta
    19501 ttatttttca ttaaaaatgc ttaagtatgc atttcaatgc accacgatag tgcaacaaac
    19561 ctggcgtgca ccatgatgat cattccatca ggtacagctt cccagcgacg tatcagtcac
    19621 cgtagacatt aaagtcgaaa tactttttcg ccatcttgtc gtaggtgccg tcctgacgca
    19681 gctcgccaag cgccttattg aaggcagccg tcagttcagc atcatcttta cgtagcccta
    19741 caccggtgcc atcaccgaag tattttttgt cttttactga tgagccagca aaggcgaaat
    19801 ctttaccagc aggttgcttg aggaatcctt cgctggcagc aacttcatct tgtaacgcag
    19861 catccagacg tcctgcagcc agatcggaat agaccaaatc ctggttggca taggccacca
    19921 catccacgcc tttgctacgc caggtctcgt tagcgtaagc ttcctgggtt gatccctgca
    19981 gcacaccaac atgtttacct ttcagtgaat ccagcgttgg ctgaatcggt gaacctttgg
    20041 cagcaatcaa acgagaatct gcggcgtaca gcttgtcgga gaaggcaatc tcctgctgac
    20101 gtttatcggt aatggaaagc gacgaaataa tggcgtcgat ttttttcgct ttcagtgagg
    20161 ggatcagcgc gtcaaagtca ctggcaaccc aggtacattt cacctgcatc cgtttgcaca
    20221 tctcgttacc gagatcgata tcaaagccaa caaaatcacc tttagcatct ttcgacgaga
    20281 acggcgcata ggtggtatcg gttccgatac gtaccgtctc cggtagcgcc gcatagctgg
    20341 aagccgctgc ggagagaccg actaacaaag acagagcgag aatcgacttc ttcatacata
    20401 accctcaagt gaaaatggct tttttatgtt gtgtattgtg ttgtgtgttt gcaggctctt
    20461 tcatgcaggt cttatgccat cttgacgaca acagtaacat tcaacgttaa atatgttaat
    20521 aagacgttgc attattgtcc tgaagttgaa gatagcaggt atagcggttg aatcgcagcg
    20581 tttcgattga atggcagaaa caaaatgtcg aggatttgat cgcggttgca aaattgccct
    20641 gaaacagggc aacagcggag ttatgcgccc tgccagcggg caaagagatc ttcaggaagg
    20701 gttatcgcaa actggtcaag aacacgatta accgtctgat ttatcacatc atcaagggat
    20761 tgcgggcgat gataaaacgc cggaacggga ggcataatca ccgcaccgat ttctgccgcc
    20821 tgagtcatta aacgcagatg gcctaagtgc aatggtgttt cacgcacgca gagcaccaac
    20881 gggcgacgct ctttcagcac cacatctgcc gcacgggtca gtaagccatc agtatagcta
    20941 tggacaatgc cggaaagggt tttgattgaa cagggtaaaa tcaccatccc tagcgtctgg
    21001 aaagaaccgg aagagatgct ggcggcaata tcgcgcgcat cgtgcgtgac atcggctaat
    21061 gcctgcactt cgcgcagaga aaaatccgtt tcgagggata aggtctggcg cgctgcctgg
    21121 ctcatcacca gatgcgtttc gatatctgtg acatcgcgca gaacctgtaa taagcgcacg
    21181 ccataaatcg cgccgctggc accgctgatg cctacaatga gtcgtttcat aaatagttgc
    21241 cctgtcagac ttgcaggcag actttgcagg atttcggcgg gagttgcaag tcagggtgcc
    21301 agaccggcac cctcagcgaa ggcatcatcc ttcgttatgc atttcgagat tttccacttc
    21361 gttctgacgt tgcactgctt tggcgtcatc attacgtaac gtatcgagga aatcgaggta
    21421 gccctgatca acatctttgg tgacgtagac gccgttgaac accgagcatt caaactgctg
    21481 gatatccgga ttttcagcgc gaacggcgtc gatcagatcg ttcagatcct ggaaaatcaa
    21541 cccgtcagca ccgatgatct ggcgaatttc atcaacttcg cgaccgtgag cgatcagttc
    21601 cgtggcgctc ggcatatcaa taccataaac gttcgggaag cgaatttccg gtgccgcaga
    21661 agcgaggtac actttcttcg ctccggcttc gcgtgccatc tcgataatct gctcagaagt
    21721 ggtgccacgg acgatggagt cgtcgaccag caggacgttt ttatcgcgga actcggcgcg
    21781 gttggcattc agtttacggc gcacggactt acgacgcagc tgctggcccg gcatgataaa
    21841 ggtgcggcca acatagcggt ttttaacgaa gccctggcgg tacggtttgc ccagaatacg
    21901 agcaatttcc agcgcgatat cacacgaggt ttctgggatc gggatcacca cgtcgatatc
    21961 cagatcttcc cattcgcggg caattttctc gcccagtttc gtgcccatat tcacacgcgc
    22021 gctgtaaacg gaaattttgt cgatgaacga gtccgggcga gcaaagtata catactcaaa
    22081 caggcacgga ttgctgaccg gattgtcagc acattgacgg gtaaacaact gcccttcttc
    22141 agtgatgtaa atcgcttcgc ccggcgcgac gtcacgcagg aaatcaaagc ccagcgtatc
    22201 gagcgctacg ctttcggaag cgaccatata ttctgtacgg ttctcgtcaa tatcacgttt
    22261 tcccagtacc agcggacgaa tcccgtttgg atcgcggaaa gcaaccatac cgtggccgat
    22321 aatcatcgcc acacaggcat atgcgccgcg gattaagcgg tttgtggcag caatggcagc
    22381 gaaaatattg tcggcttcca gcgggtagtg gcggaagttg tccagctcgc tggcgaagat
    22441 attaagcaga atttccgagt cggaagtggt gttgatgtgg cggcgttttt cttcaaacag
    22501 ttttttacgc aactcgtgag cgttggtcag attgccgttg tgggcaagcg taatgccata
    22561 cggggagtta acgtaaaacg gctgcgcttc agaggcgctg gagctgccag ccgtagggta
    22621 acgcacatga ccaatgccca tattgccctg caaacgctgc atatggcgag cttcaaatac
    22681 atcgctcacc agcccgttcg ctttacgcaa acggaagcag ttattggcat ctatggtgat
    22741 gatgccggcg gcatcctgac cgcgatgctg aagcaccgtt aaggcatcat aaatcgactg
    22801 gttaaccggc ataacaccgg cgataccgac aataccgcac atacgtcttt ttcctcgtta
    22861 agccacatct cagagcactt acgctctggg caagaaactt gacgagcttt gcagataatc
    22921 aaaaaaccat ctgatgataa aactgaactg cgggatcagc tgtgatttgc tccagtcttc
    22981 gcttttcgac accccggtaa aggagtcgag aaagaataga atggcggcaa caatcaacac
    23041 accgcgcaac gcaccgaaac agacgcccag cacccgatcg gtgcctgaca accccgtttt
    23101 ctccaccagc tggcctatca cgaagttcac gatagcacca acgatcaggg tagcgataaa
    23161 tagtaccgcg atggcaatcc catttcgaac cagttcgtct tcaaagcccg taaaccagac
    23221 tgacaggtaa gtgtagtaat gactggcaac aaagaaagca caaccccatg tcaccagcga
    23281 taacgcttca cgaacaaagc cgcggatcag gctaaccaga gaggaaaacg caatcaccgc
    23341 gattatggcg taatcaatcc agaccatatg tgtcccacga ttttacgccc tgtcatcctg
    23401 ttcggggcgc attctaacag aaaaagaaaa cgtttgcgta gggatttcct tcccgcgcat
    23461 caataaaaat ggcgctgaaa aaatattcaa cgccatcgac tttttatgcc tttgcggcat
    23521 cgtgcaatgc gtgtcggatg cggcgtaaac gccttatccg acctacggtt ctaacactgc
    23581 ccaagcctga taagacgcgc cagcgtcgca tcaggcaaga ctgtattaat tcggcgtata
    23641 gcccattacc acgccactta agccagaaag ttgcttcaac tcacccagcg aacctttcag
    23701 cttatctttc gaggcatccg gcccaaccag aatacgggta attttaccct gcactggcgt
    23761 ggatggcgac gtataaaccc gataaccggc accgcgcagc ttaccgacaa tctcattcac
    23821 tttatcggca tttttcagcg cacccagttg cacaacatag gctttacccg ttggcgctgc
    23881 tttttcttcc acgaccggct tcggttctgg cttcggcgca ggtggtgctt caaccttcgg
    23941 tttaggcggc tccaccggtt tcggcttcgg tggggcgacc ggtgcaggtt ccggttcaaa
    24001 ctcggtgtta ttggctgcaa tagtggccgg atcgagcgac ggtgctgcgg catcacctgc
    24061 ccgcacctct tccgctgcgc cttccggcgg ctgcgtcggc aacgcctggg tggcggctgg
    24121 catcatatca ggctcatcac gatcgcccgc tttcggcacc agcgggatag ccgcgaactc
    24181 atcctgataa tgttttttct gcccgtccag cagccctgga agtacaatca cccccagcgc
    24241 caccagcacg atcgtgccca ctaaccgatt ctgaaactta cttgccaccg cttctcctcg
    24301 cgtcaatcac ttccatgaca tgtgcgaccg tgtggaaaga accacacacc agcacggtgt
    24361 cttccgcttt agcgtccgcc attgcggcat cccatgcctg cgcaacgcta tcaaatgatt
    24421 tgccgttacc caaatgctca agcagttgtt ctgccgtggc accgcgcggc ccttccagtg
    24481 gcgcacaata ccagtcatca accacgcttt tcaaccaggc cagagttccg gcaatatctt
    24541 tatcatgtag cataccgata accgccagca cgcgcccgtt tttcggtagc gctttcatac
    24601 gcccggtgag atattccgcc gcatgtggat tatgcgcgac atcaaaaata acgcgtggcg
    24661 actcgctcac aatctggaaa cgtcccggca aaattgcgct ggcaatcccg tcgcgaatgg
    24721 cattttcact gacttccagc ccgctggcac gcagtgccgc cagcgctgtt gcggcattcg
    24781 gttgcgggac aagcggcaac ggcagatttt ccagcgtgcc gtgagcatcg ctaaacgccc
    24841 agtcatgatc ggtgacggaa tagttccact caacgccccg acgttgtaac agtgcacctt
    24901 tttcctgcgc cacatcagca atggtagaag gcatttccgg ctcaccgaca attgccggtt
    24961 ttgcgctgcg aaagatgcct gctttctcgc gaccaatact ttcgcgatct ggtcccagcc
    25021 agtcggtatg atccagcgca atgctggtta ctacagccac atcggcatcg acaatattgg
    25081 ttgcgtccag acgaccgccc agccctactt ccagaatcac tacgtcaagt tgtgcctgtt
    25141 tgaacagcca caacgccgac agcgtaccgt actcgaaata ggtcagggaa atatcaccgc
    25201 gtgccgattc aatctccgca aaagaggcgg tgtgggccga ttccggcaat tcctgcccct
    25261 gcacacgtac gcgctcggta taacgcacca gatgcggtga actgtagacg cccactttgt
    25321 accctgccgc catcagaatc gactccagcg tacggcaggt ggtgcctttg ccattcgtac
    25381 ccgcaacggt aaacacaaat ggcgctggtt tcagaacgcc aagacgcgcc gcgacctggc
    25441 tcacgcgctc aaggccgaga tcgatagttt tactgtgcag gttttccaga taagaaagcc
    25501 acgaagccag aggcgacgcg gcttgaggag tgcgtttgat aatcatggta tccgctgatt
    25561 cgttacggtg agaatagcaa aagggcagag ccagtggccc tgcccttatc agttatcagg
    25621 cctcaggttc ctgatccggt accgggggta ccactacgcc ttcacgcggc gcttcaggat
    25681 tcggcgctgg cagattcatc aacttcgcca gaatgctcgc cagtttcagg cgcatttccg
    25741 gacgacggac gatcatgtcg atcgcgcctt tctcgatcag gaattcactg cgctggaatc
    25801 ctggcggcag tttttcgcga acggtctgtt cgataacacg cggaccggca aagccgatta
    25861 acgctttcgg ttcagcgatg ttgagatcgc ccagcatggc gaaacttgca gaaacaccgc
    25921 ccatcgtcgg gtcggtcagc acggagatgt acggcaagcc gcgctcctgc atttttgcca
    25981 gtgccgcaga ggttttcgcc atctgcatca gcgacatcag tgcttcctgc atacgtgcgc
    26041 caccagaggc ggagaagcag atcagcgggc agtgatcttc cagcgcctgc tcaacggcac
    26101 gcacgaaacg tgcacccaca acagacccca ttgaaccgcc cataaaggcg aactcgaatg
    26161 ccgcagcgac aaccggcatt ccatacagag tgcctttcat caccaccagc gcatcttttt
    26221 cgccagtttc tttctgcgca gatgccagac ggtctttata cttcttggag tcacgaaact
    26281 tcagcacatc tttcggctca agctcgctac ccagctccac aaggcttcct tcatctaaca
    26341 ggctatgcag gcgattacgc gctgtcatac gcatgtgatg gtcacacttc ggacagacct
    26401 caagattacg ttccagctca gcgcggtata aaacctgacc gcagctatca cacttagtcc
    26461 acaccccttc aggaatgctc gccttgcggg tgggagtaat gttgctttta attcgttcaa
    26521 tccagctcat tagggacctt tctgtctgaa cctggttcga tgccagtttt atctttgggg
    26581 acgcataatg ccatttttgc ccccaacaga ccatgaatgt tgcacattaa aacataacag
    26641 cccgaaactt tggataaaaa agtggtcgaa ccgcggagtt actttttatt ttgcggcgcg
    26701 tgccgcagcg cgtttgtgac gaataaattc gaaaacccca ggcaatattg aaacaaaaat
    26761 aatgccaaca atcatcagct taaggttgct ctgaatgaag gggagcgtgc cgaagaaata
    26821 acccgcgtag gtaaaaatca gtacccacaa cagtgcgccg atcacgttat aagcggcgaa
    26881 atgacggtac gacatgtggc ccattcccgc aacaaacggc gcgaacgttc tgacgatcgg
    26941 tacaaaacgg gcgagaataa tcgttttgcc gccatgtctc tcgtaaaact gatgagtctt
    27001 atcaagataa ctgcgacgaa agattttcga atccgggtta ctgaacagtt tgtcaccaaa
    27061 taaccgtcca atggtatagt tcactgcatc accgacaata gcggcaatca acataagcac
    27121 caccatcata tggacattga gatcgttagt ttccagcgac gccaatgcac cagcaacaaa
    27181 cagcagcgaa tcaccaggca ggaatggcgt taccaccagc cccgtttcgc agaacagaat
    27241 caaaaacaga atggcataaa cccagacgcc gtactccgcg accagctctg ccagatgcac
    27301 gtcaatgtgc aggataaaat cgatgaggaa ataaatcagg tccatatttg cctatgcctt
    27361 gtactcgtca tctttcaggc tgtaactacg ttggctacag cctgaaatgt tccgagcatt
    27421 attctcgtta gtccgccaga aatagcgggc ccattggcgg ttttggaaga tcataccggt
    27481 cagggtaatc caccgcgacc agatacagcc cttccgcttt tgccgttgct gccgccagcg
    27541 ttctgtcctt tgccgccagc aactctgcta tccagctctc cggctggttg tgggcaccga
    27601 cttccatcag gctgccgaca atattcctga ccatatgatg tacaaaggca ttcgctttga
    27661 tatctaccac cacataagga ccgtgacgcg tgacattaat atgcataacg ttgcgccacg
    27721 gggttcggga ctggcactgc accgcacgga acgaggtgaa atcattctcg cccagcaagc
    27781 attgcgcagc ccgatgcatc cgttcagcgt ccagcggttc gtaaaaatgg gttacccctt
    27841 tactcagtac cgccgggcgc agccgatgat tgtagatgat gtagcgataa cggcgagccg
    27901 tggcgctaaa tcgggcatga aaatcatcag gtacagcttt aacccaacgc acggcgatgt
    27961 caccaggtaa attcgcattt acgcccaagg tccacgccgc gtctttgcgc tgcgcggtcg
    28021 tttcgaaatg cactacctgc ccggtaccgt gtacaccagc gtcagtacgc ccggcgcaga
    28081 agacggtaat gggttcgttc gccacctggg agagcgcctt ttccagcttc tcctgcacac
    28141 tgcggacttc attctgccgt tgccagccgt aatacttact gccgtcgtac tcaatgccca
    28201 gcgcaatttt ataaactggc ggttgttgct ggtcggacat tagtacagat actcctgcac
    28261 cagtttctcg gcgattttta ctgccatcag cgcgccgcca aagcgaacgt tatcggccac
    28321 cgaccagaac tggacttgct ccggcatacc gtagtcatta cgcacgcagc caacagaaag
    28381 atgcggcgta cccgaagcat cccctacctg agtcgggaat tcgttctctt cagagagcac
    28441 aatatcttcg ccctgagcaa acgcatcaca cgcttcttct gccgccagcg gacgcagtgc
    28501 ttcaaagttg accatctggg catgaccgta gaataccggt gcctggacga cgcttgccga
    28561 aatcatcagc ccttcgtcct gcaggatttt gcgtacttcg tcaacgatac gacgttcttc
    28621 acgcacgcta ccttcgctat ccggcaacaa cggcagcata ttgaacgcca gttgacgacc
    28681 gaagaaatct tcttcgtcaa tcggaatgcc gttgagcaat ttcgcactct gccccgctaa
    28741 cgcatcgacc gcttttttgc cctgggcgga ggctgaaatc aggctggtaa cactgatacg
    28801 cgacaaaccg ccctgatcga ttaacggttt cagtgccgcc agcagctggc tggtcagact
    28861 gtctggtacg gcgatgacat tccggttgcg gtaatcggtc agtacaaacg ggtttacttc
    28921 cggcaccacc agcggtacgt cgggttcgag agcaaacagt ccgctgctgt cgatcaccag
    28981 gcaacctgag ttggtcgctt cttcaaccca ggcagcggta gcttctttgc ctgcgacaaa
    29041 aaatgccagc tgcgcctgcg tccagtcgaa ttcagcggca tcctgcacgg tgattgtctt
    29101 accaccaaag cgcagttgtt cgcctgcgct ttcgttacgt gccagtgcat aaatttcccc
    29161 aaccgggaac tgacgttcag ccagcgtttc aagcagggct tcgcccacag cgccagttgc
    29221 gcccaggacg gcaatgttcc agccttcaga catggtggtt tactccagaa atagcaaagc
    29281 tccctgccaa tgttacagca gagagcatga agaagagatt aacgtgccgg atgatgaacg
    29341 gcgttaaaac ccagtttaca cagcaatgat gccgcactgg cgtcatcaca aattacatac
    29401 agagatgacc attcacggcg ctcaagatag tttttgcgca gtttatcgaa ctcacccggt
    29461 atcccggcga ctttacgcag cggtgcgtca tcgcggcgca catcatacac caaatgcacc
    29521 agccttttca gcgtcggttg atcgagcggg ccatgcagcg taatgcgacc aaactctggc
    29581 gcgggcagta atgtatccag cgcaacgtgc tgttcatgcc caataaactt gctataggct
    29641 tcaaacactt gcgtagtacc gcgtgcttta ccttccaggg tataacctgc gatatgcggc
    29701 gtgccgatat ccacttttgt cagcaactcc acgttaagtt ccggttcgcc ttcccagaca
    29761 tccagcacta cgcttaactt ctggccttca ttcaggcagg tcagcaacgc agtattatcg
    29821 acgactgcgc cacggcaggc gttaatcaga atcgctcccg gcttcaggct acggatcagt
    29881 ttttcatccg ccagatgcag cgttttgtac ggcccatctt tgaagagtgg cgtatggaaa
    29941 gtcagaatat ccgcatgctg aactaattca tccagcgagc ggaaatcacc ctcatcccca
    30001 cggtcagcgc gaggcggatc gcaaagcaag gttttaatcc ctaacgcttc cagtcgcgct
    30061 tgcaatcgac gtccaacgtt accaacgccc acgatcccca ccgtacggtc gtgcagtgaa
    30121 aatccatcgc gttcggcaag catcagcagg gaggagaaaa catattccac caccgcaatc
    30181 gcattacagc caggtgccgc ggaaaaacca attcccgcct gctttaacca tgcttcatcg
    30241 acatggtcgg tccccgctgt ggcagtacca acaaatttaa tgggttttcc tgccagcaaa
    30301 gattcattca ctttcgtgac cgaacgcacc atcagcgcat ccgcgtctgc cagttgagcg
    30361 acggggattg gacgcccggg aaccgcggtc acctcaccca aacggctaaa taagtcgcgg
    30421 gcataaggca tattttcatc aacaaggatt ttcacgtttg tgttacctgt atgagacgag
    30481 agttaaccgg acaagtgtgc cataatctcg cggccaggca tacttgcgaa gatttcaggt
    30541 ataaggatac gtaatgatac aacctatttc cggccctcct cctgggcagc caccaggtca
    30601 gggagataac ctgccgtctg gcgcgggcaa tcagccttta tccagtcagc aacgtacttc
    30661 gctggaaagc ttaatgacga aagtgacctc actgacgcaa cagcaaagag cagaactgtg
    30721 ggcgggtatc aggcacgata ttggtctgtc gggagattca ccgctgcttt cgcgtcactt
    30781 ccctgccgct gaacacaacc tggcgcaacg tctgctggcc gcgcaaaaaa gccattctgc
    30841 ccgccagctt ttagcgcaat taggggagta tttacgtctg gggaataatc gtcaggcggt
    30901 cacggattat atccgtcata actttggtca gacgccgctg aatcagctct caccggagca
    30961 attaaaaacc attctcaccc tgttgcagga agggaagatg gttattccgc aaccgcagca
    31021 gcgcgaggcg accgaccgtc ctttattacc ggcggagcac aatgcgctca aacagctggt
    31081 gaccaaactt gcggcggcaa cgggggaacc cagcaaacag atctggcaat cgatgctgga
    31141 actttccggg gtgaaagatg gcgagttaat tccagcgaaa ctgtttaacc atctggtgac
    31201 ctggctgcag gcgcgccaga cgttaagcca gcaaaatacg ccgacgctgg aatcactaca
    31261 gatggcgcta aaacaacctt tagatgccag tgaattggcg gcgttatcgg catatatcca
    31321 gcaaaaatat ggcctttctg cgcaatcatc gctttcttct gcccaggccg aggatattct
    31381 taatcagctt tatcaacggc gggttaaagg gattgatccg cgtgatatgc aaccgctgct
    31441 taatcctttt ccaccgatga tggacacgtt gcaaaatatg gcaacgcgtc ccgcgctgtg
    31501 gatactgtta gtcgcgatta tcctgatgct ggtctggctg gttcgttagc cccgacgaaa
    31561 tgacagtatc gtgacaataa ttcccagcac cgcagagatc gccccggcaa gaaataccga
    31621 agagtaacca aacgtggtcg ccagcattcc cgccagcggc ccggagacgc cgagggcgat
    31681 atcctgaaac gcggcgtaac cgcccagtgc ggtgccgcga acttgtgagg ggacgcgttt
    31741 aaccacctcc acgcccagcg caggaaagat aagcgaacat ccggctccgg ttaacgccgc
    31801 gcccgctaat gcgacccacg cccctggggc ttgccagagc agcaacaagc ccaccgtttc
    31861 tacaagcaga gagacaatcg ccactttcac gccgccaaaa cggtccggca tccagccaaa
    31921 catgacgcgc atcacgacaa atgcgccgcc aaacgcggta agagtaaagc ccgccatcgc
    31981 ccatcctttg ctggcaaagt agagcgaaac gaaagtcccg ataaccgcaa aaccaacgcc
    32041 ttgtagtgcc agccctaacc ctggtttcca gataagcccg acaacgctcc acagcgatgg
    32101 acgttctccc gccaaggccg gtactttgcg cactgtgccg ttacaggccc atgccagtaa
    32161 gggtaatacc attgtggtga tcgccagtgc ggcaaaaccg taatggctat gaatcaacag
    32221 gccaagcgga gcaccaacag cgagggcacc gtaaatcgcc attccattcc atgacatcac
    32281 tttgccagag tgttttggcc ctacgatgcc taacccccag gtcagagcgc ctgtcagtaa
    32341 ctggctttca ccaaagccaa gaatcaaacg cccgacgacc aacagggcaa atttgaacgg
    32401 tgcggagaca ggcaaaatcg ccgccagcag caacgcgccg ccagccagac cacaagctaa
    32461 catcccctga agcgccgaac gttttgcacc atattgatcg gccagtcgcc cggcatagcc
    32521 acgcgtcagc accgtagcca gaaactgaat cccgacggca atcccgacca tggtattgcc
    32581 atagcccaga tcatgatgaa caaacagcgg gataaccggc aacggcagcc ctacggtcat
    32641 gtaggtgaga aaaaccgcaa aagcgatgcg gaagagcgaa aaattggcag aagatcgtgt
    32701 ttcggtttgg cttacagcag tcatgcatta ctccagaatg cagcgcaagg cgaggagtat
    32761 ccccgtctca tctctctggt ttcagggtta cggtgcgttg gcaggattta acgcgtacgt
    32821 cttttcagaa ggaaatcgac aaagcgggaa gtttgcctgg aacaggcggc gattgtcaat
    32881 gatgtgaaaa agggaaccat caggttccct tttgcgttag tgccggaggc cgcattagcg
    32941 cgtaacgtcg gatgcgacgc tggcgcgtct tatccgacct actccgacta ctccgaccta
    33001 ctgcgaatta atctttcagc ttgcgcatta ccagcgtggc gttggtgccg ccgaagccga
    33061 agctgttaga cataacggta gtcagttcgc gatcggtcgt ttcggtcacg atgttcagac
    33121 ccgcagcctg ctcgtccagc tcttcaatgt tgatgctcgg ggcgataaag ccgtgttcca
    33181 gcatcagcag agagtagata gcttcctgta cgccagcagc gcccagagag tgaccggtca
    33241 tggctttggt tgcagaaatc gccgggctct tatcgccgaa cacttcacgg atagctgcca
    33301 gttctttcac gtcgccaacc ggagtcgaag taccgtgaga gttcaggtaa tcgattgggg
    33361 tatcaacgcc atgcatcgcc atctgcatac aacgtactgc gccttcgcca gacggagcaa
    33421 ccatgtctgc accatcagag gttgcgccgt agccaacgat ttcagcatag atgtgagcac
    33481 cacgcgccag cgcgtgttcc agctcttcaa ccactaccat accgccgccg ccagcgataa
    33541 cgaaaccgtc acggtgagcg tcgtaagtac gggaggcttt ttccggggtg tcgttgtatt
    33601 tagtagacag cgcacccatc gcgtcgaatt cgcaggccat ttcccagcac agctcttcgc
    33661 cgccgccagc aaacacgatg tcctgtttgc ccagttggat ctgctctact gcgttaccga
    33721 tacagtgtgc ggaagtcgca cacgcggagc tgatggagta gttaacgcca tgaattttaa
    33781 acggcgtggc gaggcaggca gaaacgccgg atgccatcgc tttggtgacc acatacgggc
    33841 caaccgcttt caggccgcgc gggccgcgca ttgcgtcagc gccgaatacc tggaaacgcg
    33901 gggaaccgcc gccagaacct gcaatcaggc caacgcgcgg gttattctgg taagcttccg
    33961 gagaaaggcc cgcatctgcg attgcctgct ccatagaaag gaatgcataa atggatgcgt
    34021 cgctcataaa gcgcacaact ttgcggtcaa tgaggccagt ggtatccagt tttacgttgc
    34081 cccagacgtg gctacgcatg ccggaatcct tcagctcctg agagaaagtg atccctgaac
    34141 gtccttcacg cagagatgcc aggacttcct gctggttatt accgatgctg gaaacaatgc
    34201 ccaggccagt aatcactgca cgtttcattc aatacctctg taagtcgcac atagagtaag
    34261 tttcgaatgc acaatagcgt acacttgtac gccgaacaag tccgatcagc catttaatag
    34321 agaaatttgc gcagccttac acacatcgct aagatcgagc caccgcctgt aagacgagta
    34381 acttacgtga aacactactc catacaacct gccaacctcg aatttaatgc tgagggtaca
    34441 cctgtttccc gagattttga cgatgtctat ttttccaacg ataacgggct ggaagagacg
    34501 cgttatgttt ttctgggagg caaccaatta gaggtacgct ttcctgagca tccacatcct
    34561 ctgtttgtgg tagcagagag cggcttcggc accggattaa acttcctgac gctatggcag
    34621 gcatttgatc agtttcgcga agcgcatccg caagcgcaat tacaacgctt acatttcatt
    34681 agttttgaga aatttcccct cacccgtgcg gatttagcct tagcgcatca acactggccg
    34741 gaactggctc cgtgggcaga acaacttcag gcgcagtggc caatgccctt gcccggttgc
    34801 catcgtttat tgctcgatga aggccgcgtg acgctggatt tatggtttgg cgatattaac
    34861 gaactgacca gccaactgga cgattcgcta aatcaaaaag tagatgcctg gtttctggac
    34921 ggctttgcgc cagcgaaaaa cccggatatg tggacgcaaa atctgtttaa cgccatggca
    34981 aggttggcgc gtccgggcgg cacgctagcg acatttacgt ctgccggttt tgtccgccgc
    35041 ggtttgcagg acgccggatt cacgatgcaa aaacgtaagg gctttgggcg caaacgggaa
    35101 atgctttgcg gggtgatgga acagacatta ccgctcccct gctccgcgcc gtggtttaac
    35161 cgcacgggca gcagcaaacg ggaagcggcg attatcggcg gtggtattgc cagcgcgttg
    35221 ttgtcgctgg cgctattacg gcgcggctgg caggtaacgc tttattgcgc ggatgaggcc
    35281 cccgcactgg gtgcttccgg caatcgccag ggggcgctgt atccgttatt aagcaaacac
    35341 gatgaggcgc taaaccgctt tttctctaat gcgtttactt ttgctcgtcg gttttacgac
    35401 caattacccg ttaaatttga tcatgactgg tgcggcgtca cgcagttagg ctgggatgag
    35461 aaaagccagc ataaaatcgc acagatgttg tcaatggatt tacccgcaga actggctgta
    35521 gccgttgagg caaatgcggt tgaacaaatt acgggcgttg cgacaaattg cagcggcatt
    35581 acttatccgc aaggtggttg gctgtgccca gcagaactga cccgtaatgt gctggaactg
    35641 gcgcaacagc agggtttgca gatttattat caatatcagt tacagaattt atcccgtaag
    35701 gatgactgtt ggttgttgaa ttttgcagga gatcagcaag caacacacag cgtagtggta
    35761 ctggcgaacg ggcatcaaat cagccgattc agccaaacgt cgactatccc ggtgtattcg
    35821 gttgccgggc aggtcagcca tattccgaca acgccggaat tggcagagct gaagcaggtg
    35881 ctgtgctatg acggttatct cacgccacaa aatccggcga atcaacatca ttgtattggt
    35941 gccagttatc atcgcggcag cgaagatacg gcgtacagtg aggacgatca gcagcagaat
    36001 cgccagcggt tgattgattg tttcccgcag gcacagtggg caaaagaggt tgatgtcagt
    36061 gataaagagg cgcgctgcgg tgtgcgttgt gccacccgcg atcatctgcc aatggtaggc
    36121 aatgttcccg attatgaggc aacactcgtg gaatatgcgt cgttggcgga gcagaaagat
    36181 gaggcggtaa gcgcgccggt ttttgacgat ctctttatgt ttgcggcttt aggttctcgc
    36241 ggtttgtgtt ctgccccgct gtgtgccgag attctggcgg cgcagatgag cgacgaaccg
    36301 attccgatgg atgccagtac gctggcggcg ttaaacccga atcggttatg ggtgcggaaa
    36361 ttgttgaagg gtaaagcggt taaggcgggg taatctgctc tcgcagtgtt taccggatgc
    36421 ggcgtgaacg ccttatccga cctacgtgtg acgtgtaggc ctgataagac gcgataagcg
    36481 tcgcatcagg catggtgctc cagacgccgc aacttactgc tgtgacgcct gctgaaacag
    36541 gttttcccac atatcggtca ccagcgcctg gtcacgcggc gacaattcac cggcaccaat
    36601 ggctttttcc agactctggc taacggtcgt atgcaccgcc tgagcggagt ggtcatcacc
    36661 actttccagt tctgcgatgg ctaatgtcag gtggccacgc aaatacccac tggcaaacaa
    36721 ctcatcatca ctggcatggt caaccatacc gtcgattaat gccagaatgc gtgattcaaa
    36781 ctccgcgatc atcttctttc ctcattgtaa aacgtggcgc tggctttagt tgagcgcttc
    36841 cggccacggg aactgttccg ccgtaagttc cggcgtgtga taataatttt gtagtgcttt
    36901 aatgaagcgt gccgggcgtt caggaatgcc gtttgctagg taatccatca cctgcgcatg
    36961 tacccgccgt tgaaacacca cacgatccgg ttcgaagtcg ccttccagat tgtcgcaact
    37021 gacgttaaac ggatatcccg ccgccacaca gaacaaccag tcgagtgcct gcggcttcac
    37081 ttcaacatct tcaaactggc tttgcgtttg ggcatcacgc ccatccgggc aataccagta
    37141 gccgaaatca accagctcac ggcgcgcttt tccggcaata caccagtgcg aaatctcatg
    37201 aattgcgctg gcataaaagc cgtgggcaaa gacaattcgg ttatacggta cttccgcatc
    37261 agcaggaaga tagatcggtt cgtcgtcgcc tttaatcaga cgggtattaa attcatcggc
    37321 aaagcagcta ttaaaaattt caatcaactg ctcatagtgg tgtgtactgt tcattagttc
    37381 atccccaacc agtggaggat ctcctgtccg tggctatcgt aaagtaattt ggcactcatc
    37441 accgccgaga cgataacaat catcgggcgg atcagctttt gtcctttgct caacaccagc
    37501 cgtgaaccca tgcgcgcgcc gaggaactgc cccaccagca tgacaaagcc cgtcgcccaa
    37561 atcactttgc cgccgagaat aaacagcagc agaccgccaa tgtttgacgt tgcgttgagt
    37621 aatttggcgt gagccgtggc tttggcgagg ttgaatccgc acagcgtaac gaaggccagc
    37681 gcgtaaaacg atccggcagc cgggccaaag aatccgtcat aaaaaccgac gcagccaccg
    37741 gcaattaacg cgaagggtaa accgtacatc cggcgctggc gatcttcttc acccagcttt
    37801 ggcatcagca agaaatagag gccgatacaa atcaccagaa tgggcaaaat ctgccgtaag
    37861 acatcagcct gaacgtactg taccaacagt gcgccgctca ttgagccgac aaaggtcatg
    37921 gcaatattga gtttctgatc gcttaaacta accactttgc ggcgaataaa gtagatagtg
    37981 gcggaaatag agccgccgca ggcttgcagt ttattggttg ccagcgcatt agcgggagac
    38041 atccccgctg ccatcaatgc cggaatggtg agtaacccac cgccaccggc aatcgagtcg
    38101 ataaatcccg ccagcatggc gacaaaaaag agaactccca gcaacagcgg ggaaaccata
    38161 aacaggctat taaacgtttc cattagatca cgtgctcatc cagtagcgcc tggcaggaag
    38221 gcggcaacgg aggcggtgtc ttcttctcag gctttgttgt tcccggtttt ggaggtgcaa
    38281 accagctttg cagttctgcc ccgcaaccat cgcctggtgg cggtaaaggt tgatcttcac
    38341 actccagact atcggcagga caacgtaatc gtacatgcat atgcgcgcga tgctggaacc
    38401 agggtcgcac tttgcgcaac cagtcgcgat cggtgcccgc atcaaggcaa agttgttgtt
    38461 taatcgccgg attaacaaaa atgcgtgtga cgtctttgtc ctgggcggcg agtttgatca
    38521 agctgaaaat ttctggcttc cacagcgtgg agacaacgtg tttaccgtcg cgggaaacta
    38581 agtccagtgc ttgcgggcgc aagagctgcg cggaggtcca gcgagttttc ggcagttgca
    38641 gaaagatatc gacatccagt ccggtctggt ggctggcatg accgccgttg aaacgcccac
    38701 cagcgggcat ccccatatcg ccaatcagca ccgtacccat gcccagattg ctcacctggc
    38761 tactcagacg ctggataaac atcaccagat ccgggtgacc gaaatagcga cgctgatcgg
    38821 tacgcatgac ctgataatgt tcggactgta tcggcagcgt gtcagcaccg acgatacagc
    38881 cattagaaaa actgcctatc gattgtgcgc tacccggcac aggttgggtt attttttgcc
    38941 acggcgtcgc tgccaggcta gcgctactgg caagcagagc cagcagcgca atcgcggttt
    39001 tgttcatttt ttaccagcgt ggaatatcag tcttcacatc ggcattttgc gcccgctgcc
    39061 gtaacaggtg atccattaaa acgatcgcca gcatcgcttc agcgatcggc actgcacgga
    39121 tcccgacaca gggatcgtga cggcctttgg tgatcatctc aacttcttcg ccaaagcggt
    39181 taatagtgcg ccccggcacg gtaatgctgg aggtcggttt cagcgccata tgagcaatga
    39241 tttgctgccc gctgctgata ccgccgagaa tgccgcccgc atggttgctc tggaaaccgt
    39301 ctttggtgat ttcgtcgcgg ttctggctgc cgcgcagcgc cactacgtca aaaccgtcgc
    39361 caatttccac gcctttcacc gcgttgatgc tcatcagcgc atgggcgatg tcggcatcca
    39421 ggcggtcaaa gaccggctcg ccaagtccgg cggggacacc actggcaaca acggtgactt
    39481 tcgcgccgat ggagtcgccc tcttttttca gcgcgcgcat cagttcatct aacgcgtcga
    39541 ttttgtcggg gtccgggcag aagaacggat tttgttcaac cagcgaccag tctttgattt
    39601 ccagcggaat gtcgcccatc tgggtcaagc agccgcggat ttcaatacca aatttctcgg
    39661 cgagatattt tttggcaatc gcccctgccg ccacgcgcat ggcggtttcg cgggcggaag
    39721 aacgtccacc gccgcgataa tcgcgcagac cgtatttttg ttcgtaggtg taatcagcat
    39781 ggcctgggcg gaaaacgtct ttaattgcgc tgtaatcctg agagcgctgg tcagtgtttt
    39841 cgatcaacaa gccaatgctg gtgccagtag taacgccttc aaaaacaccg gagagaattt
    39901 tgacctgatc cggctcgcgg cgctgggtgg tatagcgcga tgtcccaggg cgacgacggt
    39961 cgaggtcatg ttgcaggtcc gcttccgtca gcggaatgcc tggcggaaca ccatcgacga
    40021 tgcagccgag cgccagccca tgcgattcgc cgaaggtggt tacgcgaaag agttgtccaa
    40081 ttgtgtttcc agccatcacg gctccgttat tgttgtgttt gcgtgtttac ttaatcttta
    40141 taaatcgcga aatattcacg ggccgccagc agctgctctt tggtgagcat aaacacacca
    40201 tcgccgccgt tatcaaactc cagccaggtg aacggaacat ccggatattg ttccataaga
    40261 tgtaccatgc tgttgccgac ttcacaaatc aacacgccat catcagcaag gtaatctgcc
    40321 gcgtttccaa gaatgcgacg tgtcagttta aggccgtcgg tgccagatgc caggcccagt
    40381 tccggctcgt gacggtattc gtttggcagg tcggacatat cttccgcgtc gacatacggc
    40441 gggttagtga caatcagatc gtattgcact ttcggcaaat cacggaacag atcggaacga
    40501 atcggaatga cgttgtggat cagaccgtgt tcttcgatgt tctgttcggc aaccgccagc
    40561 gcgtcgggag agatatctac cgcgtcgact tccgcttccg ggaaggcata agcacaggca
    40621 atggcgatgc agccgctacc ggtacacata tctaaaatat gctgcggttg cttgctgatg
    40681 agtccggcaa atttattgtt gatcagttca ccaatcggcg agcgcggcac cagcacgcgt
    40741 tcatcgacgt aaaattcatg accgcagaac caggctttgt tggtcaggta agccaccgga
    40801 atgcgctcgt tgacgcggcg gatcacgcgt tcaacaatac ggtgtttttc gctggaggtc
    40861 agacgcgcgg tgcgcatatc ttccgggata tccagcggca ggtagagcga tggtaacacc
    40921 agctgtacgg cttcatccca cgggttatcg gtgccgtgac cgtaccagat atttgccgcg
    40981 ctgaagcggc ttaccgacca gcgcaacatg tcctgaatgg tttgcagctc atttactgct
    41041 tcatcaacga aaattttatc cacgtattcc tccagggcat gatcgcaata atttcggcgg
    41101 ctagtttgcc atgaagatga cgataaatca gcattcacgc gcggtgagtg aggaaaaata
    41161 cgtttaaaac gatcgattgt gctacgagtc gggtaaactg taggaaaatt agaaatagag
    41221 acagataaat gaaaaagaaa acaacactta gcgaggagga ccaggctctg tttcgccagt
    41281 tgatggcggg gactcgcaag attaagcagg acacgattgt ccaccgaccg cagcgtaaaa
    41341 aaatcagcga agtgccggtg aaacgcttga tccaggagca ggctgatgcc agccattatt
    41401 tctccgatga gtttcagccg ttattaaata ccgaaggtcc ggtgaaatat gttcgcccgg
    41461 atgtcagcca ttttgaggcg aagaaactgc gccgcggcga ttattcaccg gaattgtttt
    41521 tggatttaca cggtctgacg cagctgcagg ccaagcagga actgggcgcg ttgattgccg
    41581 cctgtcgccg cgaacatgtg ttttgtgcct gcgtgatgca tgggcacggg aagcatattt
    41641 tgaagcaaca aacaccgctg tggctggcac aacatccgca tgtgatggca tttcaccagg
    41701 caccgaaaga gtacggcggt gatgcggctt tgttggtgtt gattgaagtg gaagagtggt
    41761 tgccgccgga gttgccctga atgctggcag caaatgccgg agcagcgtaa acgccttatc
    41821 cggcctacaa atatgtgata attcaataaa ttgcaattca cttcgtaggc ctgataagac
    41881 gcatcagagc aatactacga actacagttc attcaataca tactgaaaac gacacgtcga
    41941 atcaatcggt tgcccactac tggtattcac attcaccaca atatcgtgta gtactttatc
    42001 cgttaccgga tgacgagttg gcgtaaccgt taccgttgat ttatcacaac cgtaagaggt
    42061 aaaggttaac aattctgata ccgcgcggca gtatgctcct ctgccatcat tatcagaaaa
    42121 attactgcca tcacagagtc tgccgccttc catcaagtag gtcgaaagcg tggtggtcac
    42181 tgttgacccc gtggactgaa ttcgtaacgc cttattatgg ctgacataat tttcactcca
    42241 ggagatatcg tatgaagcga ccggtatcgt tgggcatatt gtgttatgca ctgtaggggc
    42301 cgtatagctt ccaaatccgg gtaaatctgt ataggaataa aaggatgcga tccaagggca
    42361 accataatca tcagatgcaa cggtaatttt aaacgtcata tatgcacaac gaaaaatttt
    42421 cgtatctatg ccagggcaat ttgagttatt aattgtatca aagtcagtca gtaccaaagt
    42481 tggagctgaa cccgacccag accaggaaat aacccgcaac gtatcgcctt tatacgcaaa
    42541 agaatcatat ttcccatatg caacctcctt tggcgttatc accgtaacat tgaaaacact
    42601 gctcgctaca gcaccggtta ccttgtccat ggcataataa actctcacac tgtcaaaact
    42661 cgcactcata agtttatctc cagcataact cttagatgcg ccgacaacac tcagaacgag
    42721 cagcattagg ctcgcgaaaa ttatggtcca ttttttcatt tcagaaatac tcaaaggttg
    42781 ccgtcactgt ggcactaaag tctccggacg ttacatctcg cccgctcttt gcctgtaacc
    42841 aggtattaaa atttaggctg ttatttccct gattcagcgg gagcgtgacg cctgttgtat
    42901 tgttgattgg caccgatgtg cctgccgcag tttcaatacc aatcccagcg ccggatgctg
    42961 atgacgagga gtcaaaggcc agaaagccgg gtagagcact atcttctgtt cctgtcaacg
    43021 ttactttgac attaaacaat gtagaactat gacaatcttt caattgaaac accaatggca
    43081 ctcgctctga ctgcccaagg ttcattaaat cgctggcggc aatggcagga aaatgtactt
    43141 ctgccaactg gctaccatta atcactggcg tacacgattt actgattaaa ttgccggtaa
    43201 agtgcaaatt atctcctgct attgtggttc cactgcccag caacatcagg ctactaataa
    43261 aaaacaacgt acgctttttc ttcattggta atctacctgt aacgtggccc aggcctcaaa
    43321 gtccgcctct ggcagaacga caccactctt ttttactggc acagccgtta acaccggtaa
    43381 atcggtttca ttcaccacaa gtggcgtatt gatcgtaaaa ctctgcccta cctgtttgag
    43441 ctgaatgcct aaaccagtga tattgctgct tactgcagca ttatcaaaat cgcttggtgt
    43501 gccactccag ctcaaggtca tttccattaa tgggtcgcga accgccgtat cgcaggtaat
    43561 ggaatacggc acggtttgct cataattgct gccattaatg tcatcaataa gcacatcctg
    43621 aaaagttatt tcaatggttt gagcgttgct aatcgtgcag ttaggtggct gcactaaata
    43681 accgtgaaaa gagacgtttg agagtgccat cgagcaaaag ccccacagca aaataagcga
    43741 gatccgtttc attggtaatc caccacaatt gtggcactgg cgttaaattc agctgccgct
    43801 aacgttgtac cactctgttt caccggcaca gcttccagaa cgggaactcc cgaggaaaaa
    43861 ttaaatggca accatgagcc agaggttaag tcgagtattg tgtgatcact acttttctga
    43921 acccgaatac caaacccagg aatccccgtg ctgataactg tttcaccgtt aatcacgacc
    43981 gttgtcgcct gcatctgtag acgcaggtca tccattgcca gattgttaca aacaagcgag
    44041 tagtcaatgg gacgtttata atcaacccca ttgatcttat taatgaatac attaccaaac
    44101 tcaactgccg caccatttac cgtacaagga ggaggggcat cgacaagcac ccgtaaattc
    44161 atacttactg acttactcgc tgcaactgca aagccgctac caacacaaca tatcagtaac
    44221 ccagttaccg cccagacaga acaagatcct ttcatactgg ctctcccggc ctcagccttt
    44281 gcggctctgc tcttcatcga cctggcaact gttaccgttg caagtgaaaa agagcggcat
    44341 ccgagcgcca tagtcgttga cataggtcag taccggcacg ctacccatct tcacattcag
    44401 cggtgcagag gttttcggcg acatcactat tggcgagaaa ccggctgccg gattgccatt
    44461 tttctgtgtg ctggcattac tgaaaatgac gtaatacggt gtcgggttat tgacggtaaa
    44521 ttcctgaccg ttgcgggtta aagtaacctt atattgccag gggtttttca tgctgacgtt
    44581 ctccagcgcc ttcggtcgcc agaacaactt aatacgcgtt tgtagtgcta tttgcagcgt
    44641 gttggctttg tttgattttg gcgggatctc acgcacgttg aagtagaaca ggctttcacg
    44701 atcggcaggc aatttgttga tatccggcat tccctgcact tttacctgcc cgttcatcat
    44761 tgaatcgatg cgttgcaccg gtggcagaac agttaacggt gaactgatct tgttaccctt
    44821 ttcatcttcg atccagcttt gggccagata aggcagtttc ggatcgttat tgcgcaacgt
    44881 cacgctgatt gatttgtcgc tttcattaaa tatcaaacgg gtacgatctg gcgttacaga
    44941 agcctgggca gacaaactgg cagcgctaag cagtaacgct agcgtcgtag cacccaattt
    45001 ggcagagcaa agtaaatcag acatggatca ttccttaatc acgttattgg ttagctgaaa
    45061 cagaaacagg gggattggtg ggtgtcacct gttgcgtctg ctcctgaatg acaggcttta
    45121 tgtcatcagg aacaaccggc gcgacgttgc ctttatgctg gcatggcagt aacaagccgt
    45181 taaacagatc ggcaggcagc gggtccggca ggttgatatc gcaatgcgca acaccactcc
    45241 agaacacact catgtgttcg ccaggtttca ccccagccag ataaacattg ccgtcatcat
    45301 cgacaaggcc cactgtctgc tcgttatcat tttttacttc tgcgccaaac ggaggatggc
    45361 tgccgtcttg taaacgcagc accgccatcg ctttttgacc actgatgacg gcaaatttgc
    45421 ggtagccaat cgccccttca gttagcgtgg cttgtaccac cgactgggtt gcttcggcgt
    45481 tttccggcag tttgttgagg tcgatatacg cctgattgcg gtaatagtta ttgacgtcag
    45541 aaacgacggc tttaccaaac atattggtat aaacagcagc accgttacct tctaccggaa
    45601 catcggcaac gccatcggca tcaatcaaca agcgtgtccc gcccatattc tgggtacggt
    45661 gaagtgcgcc accgtgggca gtaagcgttg cgccgccctg taacgacaag cccgcagagg
    45721 tgtattgccc ttcatggtag ttcgcgctga ggtcaacctg cgccagcgaa ccatcatggc
    45781 tgtaatagcc gtcaacgctg gtgtgtttgt cactggtgcc gacgttcaac tgatagtgag
    45841 tcgcgtcatc gacacggctg aaataaccga cctgactgct gtccgtccca ctgccatagt
    45901 tgccgttata gctaacggta ctgttgtcgc cccacggcat actgagcgaa atgtacatgc
    45961 ccttatccgc ccggttgtca tactcgtagc ggtagccagt cagggaaacg ctcatattac
    46021 gaatgctgcc catattgaaa tagtgggaga gcatgatgtt gtagtttgtc tgctcctcac
    46081 gatcccagta agtatggcgg gtatagttga gataaaccga aacacccgca tcgcggaagt
    46141 tctggttata agtggcggtg tacatctctt tgtcgttgcc cgtgcgtacc attccgctgt
    46201 cactggcatc cagatactcg ctcatggtca taaagttctc ttccgagaag cgatatccag
    46261 caaaagtaac gcggctgttg agctggtcaa agtctttgga ataactcaca cggaaggagt
    46321 taccgtccag cgaacctttg ccataagcgg tgtctttatc cagtttggtg tgcgagtgag
    46381 taacatcaaa cgcgaccgcg ccgaacgtag acaaatcgcg accaacgcca agtgcggcag
    46441 attggtagtt ttcatccccc agcgcgccac catagagcga ccaaccatta gcgatccccc
    46501 aggaagcttc agcaccagag aaaaattcgc cttcaacatg atgcccccac tcttgcggac
    46561 gacccatcat gatcttatag cgcacctgac ctggacgagt gaggtatggc atcgaggcgg
    46621 tgctgatgtc atattcctgc acctggccgt tctgttcttc aatgcgaata tgcaacgtac
    46681 cggagacaga atcaccaaga tcctgaatac gaaacggtcc agccggaacc tgcgtttcgt
    46741 aaatcacacg ccccatctgg ctgacggtca cttttgctgt ggtgtgtgcc acgccggaaa
    46801 tgtctggcgc gtagccgcgc aggttgggag gcaacatttg atcgtcagtg ctgacactgc
    46861 caccaacata attaaaaccg tcgaaaatat cggaattgag gtaatcttcg cccagcgcca
    46921 gtttggcttt taatgacggt aacgcccgcc aggcataata gcgactccac tcccattttt
    46981 tttgtgtgtc atcgccgcta aattcctcgt catcatcatt actgcgggta tgttgatagt
    47041 tggtctgcca gtcagcgcgc atacgccatg gccccaggtt aaccccgacc gtcccgttgc
    47101 cgctgatctc gttactatca tcaccgccat tttcttcgtg ccgggtttgt gcgttgatgc
    47161 tgtagtccgc gacgatcccg gagatgccgt catcccaacg tgaaggcgga tcccagtcgg
    47221 gataagtata ttcgaggtaa gcctgtggca gtgaaatgac taatgcggac tggcttaaat
    47281 cagccttaat ttccatgcct tccagttgac cggatttcag gcatttagca tcgtggctcc
    47341 attgcagatt tttcgccacg tcttctttta aaccaaactg cgctaccagt tccggtgtca
    47401 gacaagcata gcttttgctc gcgtcatctt caccagcata ccagtaaata tcgtactctt
    47461 ccgccaatgg ctgtttattt agttgaacct gtaaattgta ttttccgggc tcaacatatc
    47521 cctggctgga aaaacgcttc agatcaattt ttgtgtcgcc ttttaattcc agaaaacggg
    47581 aatcaaactg aatgtcgtct tcagcccaga cactgctata actccctgac attgccagcg
    47641 caatgcacca cggaagaatc cgtaatcgaa aaagagaatg gtcaggcata cacgtttcta
    47701 tccgttttta ttcttgttcg gtcaaaatcc atttaaatcc cccatagtag tgtcgctatg
    47761 ggggtatgcc ttacaggtaa gtaatctgga aggtggtatt tgcttcgaaa ttacccagat
    47821 ctggcgcatc agctgcgccg accagccagg ctttaaagtt cagtgtctgc ttcgctttgc
    47881 ctttattggt agaagtgtct ttcactattt tctggtcaat acccatgccg cttttgtaag
    47941 aggtacccag agagtcaccg atcgccaggc taacattgtt aaatgccgca ccggtatcgg
    48001 tattgaaaat ggtgtaataa ttgccgctat ttgcagaaga aacggtacca gtaaaggtag
    48061 tagtcattgt ttcctgagta tcaaatacgc aatcttgcag acgaatctgg aaacttttcg
    48121 gcgtggtggt gccgttattt ttcagttttt cggcaccgat atcacccata tcaacttcca
    48181 gtttgtgatc gtccggaacg atagaacaca ctgagtcttc aatggtgccg taaaaacgtg
    48241 cagtaccgtt gttacccgca gcgattgttg ccgtagaggt gaacacgccc atcaccattg
    48301 ccgcagcaat agctgtttta acaaacttac tcattatttg tccttaaact ataaataatt
    48361 aaaaatataa acattgcaat acattgattc agtcaatagc caatgtttat acccagccat
    48421 tgaatacggc atgcgatcca aaaagaacag gtcgtagaaa attgtattcg ttggtgtggg
    48481 gccgcatagt
//
LOCUS       DBANUU010000019        48382 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000019 DBANUU010000000
VERSION     DBANUU010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.019"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..218)
                     /locus_tag="ACIE68_001055"
     CDS             complement(<1..218)
                     /locus_tag="ACIE68_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5907405.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPF"
     gene            complement(378..590)
                     /gene="rpmE"
                     /locus_tag="ACIE68_001056"
     CDS             complement(378..590)
                     /gene="rpmE"
                     /locus_tag="ACIE68_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFX5907406.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            793..2991
                     /gene="priA"
                     /locus_tag="ACIE68_001057"
     CDS             793..2991
                     /gene="priA"
                     /locus_tag="ACIE68_001057"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFX5907407.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQVGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3147..4172
                     /gene="cytR"
                     /locus_tag="ACIE68_001058"
     CDS             3147..4172
                     /gene="cytR"
                     /locus_tag="ACIE68_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFX5907408.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4264..5223
                     /gene="ftsN"
                     /locus_tag="ACIE68_001059"
     CDS             4264..5223
                     /gene="ftsN"
                     /locus_tag="ACIE68_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFX5907409.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5316..5846
                     /gene="hslV"
                     /locus_tag="ACIE68_001060"
     CDS             5316..5846
                     /gene="hslV"
                     /locus_tag="ACIE68_001060"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFX5907410.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5856..7187
                     /gene="hslU"
                     /locus_tag="ACIE68_001061"
     CDS             5856..7187
                     /gene="hslU"
                     /locus_tag="ACIE68_001061"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFX5907411.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7254..8180
                     /gene="menA"
                     /locus_tag="ACIE68_001062"
     CDS             7254..8180
                     /gene="menA"
                     /locus_tag="ACIE68_001062"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFX5907412.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8273..8758
                     /gene="rraA"
                     /locus_tag="ACIE68_001063"
     CDS             8273..8758
                     /gene="rraA"
                     /locus_tag="ACIE68_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFX5907413.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8843..9088)
                     /gene="zapB"
                     /locus_tag="ACIE68_001064"
     CDS             complement(8843..9088)
                     /gene="zapB"
                     /locus_tag="ACIE68_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFX5907414.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9514..10359
                     /gene="glpF"
                     /locus_tag="ACIE68_001065"
     CDS             9514..10359
                     /gene="glpF"
                     /locus_tag="ACIE68_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFX5907415.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10382..11890
                     /gene="glpK"
                     /locus_tag="ACIE68_001066"
     CDS             10382..11890
                     /gene="glpK"
                     /locus_tag="ACIE68_001066"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFX5907416.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            12025..13035
                     /gene="glpX"
                     /locus_tag="ACIE68_001067"
     CDS             12025..13035
                     /gene="glpX"
                     /locus_tag="ACIE68_001067"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFX5907417.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13132..13878
                     /gene="fpr"
                     /locus_tag="ACIE68_001068"
     CDS             13132..13878
                     /gene="fpr"
                     /locus_tag="ACIE68_001068"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFX5907418.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13883..14311)
                     /gene="uspD"
                     /locus_tag="ACIE68_001069"
     CDS             complement(13883..14311)
                     /gene="uspD"
                     /locus_tag="ACIE68_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFX5907419.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14338..14637)
                     /gene="yiiS"
                     /locus_tag="ACIE68_001070"
     CDS             complement(14338..14637)
                     /gene="yiiS"
                     /locus_tag="ACIE68_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFX5907420.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14849..15289)
                     /gene="yiiR"
                     /locus_tag="ACIE68_001071"
     CDS             complement(14849..15289)
                     /gene="yiiR"
                     /locus_tag="ACIE68_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFX5907421.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15390..15989
                     /gene="yiiQ"
                     /locus_tag="ACIE68_001072"
     CDS             15390..15989
                     /gene="yiiQ"
                     /locus_tag="ACIE68_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFX5907422.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16097..16864
                     /gene="tpiA"
                     /locus_tag="ACIE68_001073"
     CDS             16097..16864
                     /gene="tpiA"
                     /locus_tag="ACIE68_001073"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFX5907423.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16919..17674)
                     /gene="cdh"
                     /locus_tag="ACIE68_001074"
     CDS             complement(16919..17674)
                     /gene="cdh"
                     /locus_tag="ACIE68_001074"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFX5907424.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVRKQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17781..18770)
                     /gene="sbp"
                     /locus_tag="ACIE68_001075"
     CDS             complement(17781..18770)
                     /gene="sbp"
                     /locus_tag="ACIE68_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFX5907425.1"
                     /translation="MNKWGVGLTFLLAATNVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19089..20051)
                     /gene="pfkA"
                     /locus_tag="ACIE68_001076"
     CDS             complement(19089..20051)
                     /gene="pfkA"
                     /locus_tag="ACIE68_001076"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFX5907426.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20232..21134)
                     /gene="fieF"
                     /locus_tag="ACIE68_001077"
     CDS             complement(20232..21134)
                     /gene="fieF"
                     /locus_tag="ACIE68_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFX5907427.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21283..21783)
                     /gene="cpxP"
                     /locus_tag="ACIE68_001078"
     CDS             complement(21283..21783)
                     /gene="cpxP"
                     /locus_tag="ACIE68_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFX5907428.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21933..22631
                     /gene="cpxR"
                     /locus_tag="ACIE68_001079"
     CDS             21933..22631
                     /gene="cpxR"
                     /locus_tag="ACIE68_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFX5907429.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22628..24001
                     /gene="cpxA"
                     /locus_tag="ACIE68_001080"
     CDS             22628..24001
                     /gene="cpxA"
                     /locus_tag="ACIE68_001080"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFX5907430.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24107..24781)
                     /gene="yiiM"
                     /locus_tag="ACIE68_001081"
     CDS             complement(24107..24781)
                     /gene="yiiM"
                     /locus_tag="ACIE68_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFX5907431.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24930..25913)
                     /gene="kdgT"
                     /locus_tag="ACIE68_001082"
     CDS             complement(24930..25913)
                     /gene="kdgT"
                     /locus_tag="ACIE68_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFX5907432.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPIITSIWSRKVKARAAKIEILGTVK"
     gene            complement(26173..26793)
                     /gene="sodA"
                     /locus_tag="ACIE68_001083"
     CDS             complement(26173..26793)
                     /gene="sodA"
                     /locus_tag="ACIE68_001083"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFX5907433.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27078..28112
                     /gene="rhaT"
                     /locus_tag="ACIE68_001084"
     CDS             27078..28112
                     /gene="rhaT"
                     /locus_tag="ACIE68_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFX5907434.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLITHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28109..28957)
                     /gene="rhaR"
                     /locus_tag="ACIE68_001085"
     CDS             complement(28109..28957)
                     /gene="rhaR"
                     /locus_tag="ACIE68_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFX5907435.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(29031..29867)
                     /gene="rhaS"
                     /locus_tag="ACIE68_001086"
     CDS             complement(29031..29867)
                     /gene="rhaS"
                     /locus_tag="ACIE68_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFX5907436.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVAEQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30155..31624
                     /gene="rhaB"
                     /locus_tag="ACIE68_001087"
     CDS             30155..31624
                     /gene="rhaB"
                     /locus_tag="ACIE68_001087"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFX5907437.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLKERKINDLPALIVATQSLPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVARIHSTRKTKELCA"
     gene            31621..32880
                     /gene="rhaA"
                     /locus_tag="ACIE68_001088"
     CDS             31621..32880
                     /gene="rhaA"
                     /locus_tag="ACIE68_001088"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFX5907438.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            32963..33787
                     /gene="rhaD"
                     /locus_tag="ACIE68_001089"
     CDS             32963..33787
                     /gene="rhaD"
                     /locus_tag="ACIE68_001089"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFX5907439.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWSFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33797..34111
                     /gene="rhaM"
                     /locus_tag="ACIE68_001090"
     CDS             33797..34111
                     /gene="rhaM"
                     /locus_tag="ACIE68_001090"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFX5907440.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34412..34858
                     /gene="frvA"
                     /locus_tag="ACIE68_001091"
     CDS             34412..34858
                     /gene="frvA"
                     /locus_tag="ACIE68_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFX5907441.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            34869..36320
                     /gene="frvB"
                     /locus_tag="ACIE68_001092"
     CDS             34869..36320
                     /gene="frvB"
                     /locus_tag="ACIE68_001092"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFX5907442.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTLVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            36310..37380
                     /gene="frvX"
                     /locus_tag="ACIE68_001093"
     CDS             36310..37380
                     /gene="frvX"
                     /locus_tag="ACIE68_001093"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFX5907443.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAAFKNCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            37380..39128
                     /gene="frvR"
                     /locus_tag="ACIE68_001094"
     CDS             37380..39128
                     /gene="frvR"
                     /locus_tag="ACIE68_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFX5907444.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNSVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNCITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(39178..40233)
                     /gene="yiiG"
                     /locus_tag="ACIE68_001095"
     CDS             complement(39178..40233)
                     /gene="yiiG"
                     /locus_tag="ACIE68_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFX5907445.1"
                     /translation="MKRNLLSSAIIVAIMSLGLTGCDDKKAETETLPPANSQPAAPAP
                     EAKPTEAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADW
                     LKDFKQGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAV
                     ALGNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKR
                     QLAELKNIEEREGKTFHYYSLAVMISAKQINNLISQDKFDAEAAMKKVSELETLVAQA
                     KEADKGGMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFP
                     RALREYNEMVDDYNSLR"
     gene            complement(40386..41219)
                     /gene="fdhD"
                     /locus_tag="ACIE68_001096"
     CDS             complement(40386..41219)
                     /gene="fdhD"
                     /locus_tag="ACIE68_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFX5907446.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDGALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            41413..44463
                     /gene="fdnG"
                     /locus_tag="ACIE68_001097"
     CDS             41413..44463
                     /gene="fdnG"
                     /locus_tag="ACIE68_001097"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:41998..42000,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFX5907447.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            44476..45378
                     /gene="fdoH"
                     /locus_tag="ACIE68_001098"
     CDS             44476..45378
                     /gene="fdoH"
                     /locus_tag="ACIE68_001098"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFX5907448.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNVGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            45375..46010
                     /gene="fdoI"
                     /locus_tag="ACIE68_001099"
     CDS             45375..46010
                     /gene="fdoI"
                     /locus_tag="ACIE68_001099"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFX5907449.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            46007..46936
                     /gene="fdhE"
                     /locus_tag="ACIE68_001100"
     CDS             46007..46936
                     /gene="fdhE"
                     /locus_tag="ACIE68_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFX5907450.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKDPKIEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(47266..47508)
                     /gene="yiiF"
                     /locus_tag="ACIE68_001101"
     CDS             complement(47266..47508)
                     /gene="yiiF"
                     /locus_tag="ACIE68_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFX5907451.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(47726..47944)
                     /gene="yiiE"
                     /locus_tag="ACIE68_001102"
     CDS             complement(47726..47944)
                     /gene="yiiE"
                     /locus_tag="ACIE68_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFX5907452.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(48321..48382)
                     /locus_tag="ACIE68_001103"
                     /pseudo
     CDS             complement(48321..48382)
                     /locus_tag="ACIE68_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        11808 a        12566 c        12483 g        11525 t
ORIGIN      
        1 atgaacggca gcgggccggg cagggcgatg tcggtttcac cgggaaggac ctttgcaccg
       61 agcagcggat tgaccggatg gccggacgtc accccgccgg ggcacaccga acaggccacg
      121 ccggtggggg caccgatgcg caccccggct gaaccctgga caatgctacc gccatactgc
      181 gtcatgtcgc cctgacgcgc cgccggtttt ccgctcatcg taaaatcctt ttgaaataaa
      241 taagaaccag aagcctagcg ccagtcaaca acaggaaatg ataatgagtt aacaccccgg
      301 aatttttcag aatttataaa aggcaaatta caggcacaaa aaaagcgccg tgcggcgctt
      361 ttttcggaaa tccggtctta tttgctgccc gggatgttga aacgcttgtt gaagcggtca
      421 acacggccac cggtagcaac atcacgctgt ttgccagtga agaacgggtg gcacttgctg
      481 cacacgtcga ggttcaggtc atgaccaacg gtggagcgga ttttcattac gttaccgcaa
      541 gagcagctag cagtaatttc ttcgtatttc gggtgaatat cttttttcat gggaaaacct
      601 cggtttaagg ccgcgtcgct cttccagccc taacgccaga caccacgcga tgttaaaagt
      661 atagcttcaa tacgatcatt tcgtacgaag gcgcgaaatc atacagaaat taaccagcgt
      721 atgcaaactg atccgcactc ttctacggca atgtgtatac taacccaccg aatttcaagt
      781 caggatgatg ctatgcccgt tgcccacgtt gccttgcccg ttccgcttcc tcgtaccttt
      841 gactatctgc tgccagaagg catgacggtt aaagctgggt gtcgcgtgcg cgtgccgttt
      901 ggcaaacagc aggagcgcat cgggattgtg gtatcagtta gcgatgccag cgaactgccg
      961 ctcaatgagc taaaagcggt agtcgaagtg ctggatagtg agccggtgtt tactcactcc
     1021 gtctggcgat tgctgctatg ggcggcagat tactatcatc atccgattgg cgatgtgctg
     1081 tttcatgcct tgccgatttt actacgccag gggcggcctg cggcgaacgc gccgatgtgg
     1141 tactggtttg ccactgaaca aggtcaggcg gtggatctga acagcctgaa acgctcaccc
     1201 aagcaacaac aggcgctggc agcgttacgg caaggcaaaa tctggcgcga ccaggttgcc
     1261 acgctcgaat ttaatgatgc cgcgttgcag gcgctgcgca aaaaaggtct gtgtgattta
     1321 gcaagtgaaa caccagagtt tagcgactgg cgaacgaact atgccgtttc tggtgagcgg
     1381 ttgcgattga ataccgaaca ggccaccgcc gttggcgcaa ttcatagtgc ggcggatacc
     1441 ttctctgcct ggctgctggc gggcgttacc ggttccggta aaacggaggt ttatctcagt
     1501 gtactggaaa acgtgctcgc tcagggcaaa caggcgctgg tgatggtgcc ggaaatcggc
     1561 ctgacaccgc aaactatcgc ccgttttcgt gaacgtttta atgcccccgt agaagttctg
     1621 cattccggct tgaacgacag cgagcgtctt tcggcgtggt tgaaagcgaa aaatggtgag
     1681 gcggcgattg tgatcggcac ccgctccgcg ctgtttacgc cgtttaaaaa tctcggtgtg
     1741 attgtcattg atgaagagca cgacagttcc tacaagcagc aggaaggctg gcgctatcat
     1801 gcccgcgacc tggcggttta tcgtgcgcac agcgagcaaa tcccgattat tcttggctcc
     1861 gcaacacccg cactggaaac gttatgcaac gtccagcaga aaaaataccg cctgctgcgc
     1921 ctgacgcgtc gggcggggaa tgcgcgtccg gcaattcaac acgtgctgga tttaaaaggt
     1981 cagaaggtgc aggtggggct ggctccggcg ttaatcaccc gtatgcgcca gcatttacag
     2041 gccgacaacc aggttatcct ctttcttaac cgccgtggct ttgcgcctgc cttgctgtgc
     2101 cacgactgcg gctggattgc cgaatgtcca cgttgcgatc actactacac gctgcatcag
     2161 gcgcaacagc atttgcgctg tcaccactgt gacagccaac gcccggtgcc gcgccagtgc
     2221 ccttcctgcg gttccacgca cctagtaccc gtggggctgg gcaccgaaca gcttgaacag
     2281 acgcttgcgc cgatgttccc cggcgtgccc atttcacgta tcgaccgcga taccaccagc
     2341 cgcaaagggg cgctggaaca gcaactggcg gaagtgcatc gcggcggcgc gaggatcttg
     2401 attggcacgc aaatgctggc gaaaggtcac cactttccgg atgtgacgct ggtggcgtta
     2461 ctggatgtgg acggcgcgct gttttctgcc gattttcgct cggcagaacg tttcgcccag
     2521 ctttacaccc aggtcgccgg tcgtgccggg cgtgcgggta aacagggcga agtggtgctg
     2581 caaacgcacc atccggaaca tcctctgttg caaacgttgc tctataaagg ctacgacgcc
     2641 tttgccgaac aggcgctggc tgagcgacgc atgatgcagc tgccgccgtg gaccagccat
     2701 gtgattgtgc gtgcggaaga tcataacaat cagcatgcgc cgctgttcct gcaacaactg
     2761 cgtaatctga tcctttccag cccgctggca gacgacaaac tgtgggttct cggcccggtt
     2821 ccggctctgg cacctaagcg tggtggccgc tggcgctggc agatcttgtt gcagcaccct
     2881 tcccgcgtgc gcttgcaaca catcattagt ggtacgctgg cgcttattaa tacaataccg
     2941 gattcccgca aggtgaaatg ggtgttggat gtcgatccca tcgaaggcta atgcgcaaac
     3001 gttgcgaggc ggatcgaaaa attcaatatt catcacactt ttcatgaaaa ttctgtaacc
     3061 gttttcacgc gctatctgct aaaaatgttg ccgatgtgaa gtaaacatgg atgtagtacg
     3121 cctgacgtgc caggcgagga gtgagtgtga aagcgaagaa gcaggaaact gccgcgacca
     3181 tgaaagacgt tgccctcaag gcaaaagtct ctacagcgac tgtctcccga gcattaatga
     3241 atcccgataa agtctcccag gccacccgta atcgggttga aaaagcggcc cgggaagtgg
     3301 gttatttacc gcaacctatg gggcgcaacg tcaagcgtaa tgaatcccgc accattctgg
     3361 tgattgtccc ggatatctgc gatcccttct ttagcgaaat tattcgcggt atcgaagtta
     3421 cggcggcaaa tcacggatat ctggtgctga ttggcgactg tgcgcatcaa aatcagcagg
     3481 aaaaaacctt tatcgatttg atcatcacca agcaaattga tggcatgttg ctgctgggct
     3541 caaggctgcc gtttgatgcc agcattgagg aacagcgtaa tctgccgccg atggtgatgg
     3601 cgaacgaatt tgcaccggaa ctggagctgc ctaccgttca tatcgacaat ctgaccgccg
     3661 catttgatgc agtaaattat ttatatgagc aagggcataa acggattggc tgtatagccg
     3721 gtcccgaaga gatgccactg tgtcactacc gcctgcaagg ctatgttcag gcgctgcgtc
     3781 gctgcggcat tatggttgat ccgcaataca tcgcccgtgg cgacttcacc ttcgaagccg
     3841 gaagcaaagc gatgcagcag ctgcttgatc ttccacaacc gcctactgct gtcttctgcc
     3901 atagcgatgt gatggcgctc ggcgcacttt ctcaggcaaa acgccagggg ctgaaagtcc
     3961 cggaagacct ttccataatc ggttttgata acatcgacct gacgcaattt tgtgatccgc
     4021 cgctgacaac catcgcgcag ccgcgttacg aaatcggtcg ggaagctatg ctgttattgc
     4081 ttgatcaaat gcaggggcaa cacgttggca gtggctctcg tttaatggac tgcgaactta
     4141 tcatccgggg atcaacacgc gcgttacctt aaagtaaacc tttaagactg ccgtgactgg
     4201 tcaaaggccc gccgcttaag taacatggcg ggctgacgaa cgaataaata cagcgaaacg
     4261 atagtggcac aacgagatta tgtacgccgc agccaaccgg caccttcgcg gcgaaaaaag
     4321 agcacctcac ggaaaaagca acgaaatctg cctgcggttt ctcccgctat ggtcgctatt
     4381 gctgccgccg ttcttgtgac ctttatcggt ggtctgtact tcattacgca tcacaagaaa
     4441 gaagagtccg agacgctgca aagccagaaa gtgaccggaa acggactacc accaaaacca
     4501 gaagaacgct ggcgctacat caaagagctg gaaagtcgcc agccgggtgt gcgtgcgccc
     4561 acagaacctt ctgctggtgg tgaagtgaaa acgccggagc aactgacacc agaacaacgt
     4621 cagcttcttg aacaaatgca ggctgatatg cgccagcagc caacgcagct ggttgaggtt
     4681 ccgtggaatg aacagacgcc ggaacagcgt cagcaaacgc tacagcgcca acgtcaggcg
     4741 cagcaattag cggaacagca acgcctggct cagcagtcgc gtacgactga acaaagctgg
     4801 cagcagcaga cgcgtacgtc tcaagccgcg ccggtacagg cgcaaccgcg ccagtctaaa
     4861 ccggcttcta ctcagcagcc gtaccaggat ctgctgcaaa cccctgcgca cacaaccgcg
     4921 cagtcgaagc cgcagcaagc tgcgccggtt actcgtgccg ctgacgcgcc aaaaccgacg
     4981 gcggagaaaa aagacgaacg ccgctggatg gtgcagtgcg gttcgttcag aggcgcggaa
     5041 caggcagaga cggtacgtgc tcagctggcg ttcgaaggat ttgactcgaa aatcaccacc
     5101 aacaatggct ggaatcgtgt ggtcattggc ccggtgaaag gcaaagagaa cgcagacagc
     5161 accctcaatc ggttgaagat ggcgggtcat acaaactgca ttcggctcgc cgccgggggt
     5221 tgaaaccctc aaaatccccc ccatctataa ttgcattatg ccccgtactt ttgtacgggg
     5281 tttgtactct gtattcgtaa ccaaggggtc agctcgtgac aactatagta agcgtacgcc
     5341 gtaacggcca tgtggtcatc gctggtgatg gtcaggccac gttgggcaat accgtaatga
     5401 aaggcaacgt gaaaaaggtc cgccgtctgt acaacgacaa agtcatcgcg ggctttgcgg
     5461 gcggtactgc ggatgctttt acgctgttcg aactgtttga acgtaaactg gaaatgcatc
     5521 agggtcatct ggttaaagcc gccgttgagc tggcaaaaga ctggcgtacc gatcgcatgc
     5581 tgcgcaaact tgaagcactg ctggcagtcg cggatgaaac tgcatcgctt atcatcaccg
     5641 gtaacggtga cgtggtgcag ccagaaaacg atcttattgc tatcggctcc ggcggccctt
     5701 acgcccaggc tgcggcgcgc gcgctgttag aaaacactga acttagcgcc cgtgaaattg
     5761 ctgaaaaggc gttggatatt gcaggcgaca tttgcatcta taccaaccat ttccacacca
     5821 tcgaagaatt aagctacaaa gcgtaaggat ctcccatgtc tgaaatgacc ccacgcgaaa
     5881 tcgtcagcga actggataag cacatcatcg gccaggacaa cgccaagcgt tctgtggcga
     5941 ttgctctgcg taaccgctgg cgtcgcatgc agctcaacga agagctgcgc catgaagtga
     6001 ccccgaaaaa tatcctgatg atcggcccga ccggtgtcgg taaaactgaa atcgcccgtc
     6061 gtctggctaa gctggcgaat gcgccgttca tcaaagttga agcgaccaaa ttcaccgaag
     6121 tgggctacgt cggtaaggaa gtggattcta ttattcgcga tctgaccgat gccgccgtga
     6181 aaatggtacg cgtccaggct atcgagaaaa accgttatcg cgctgaagaa ctggcagaag
     6241 aacgtattct cgacgtactg atcccacctg ctaaaaacaa ctggggacag accgaacagc
     6301 agcaggaacc gtccgctgct cgtcaggcat tccgcaaaaa actgcgtgaa ggccagcttg
     6361 atgacaaaga aatcgagatc gatcttgccg cagcaccgat gggcgttgaa attatggctc
     6421 ctccaggcat ggaagagatg accagccagc tgcagtccat gttccagaac ctgggcggcc
     6481 agaagcaaaa agcgcgtaag ctgaaaatca aagacgccat gaagctgctg attgaagaag
     6541 aagcggcgaa actggtgaac ccggaagagc tgaagcaaga cgctatcgac gctgttgagc
     6601 agcacgggat cgtgtttatc gacgaaatcg acaaaatctg taagcgcggc gagtcttccg
     6661 gtccggatgt ttctcgtgaa ggcgttcagc gtgacctgct gccgctggta gaaggttgca
     6721 ccgtttccac caaacacggg atggtcaaaa ctgaccacat tctgtttatc gcttctggcg
     6781 cgttccagat tgcgaaaccg tctgacctaa tcccggaact gcaaggtcgt ctgccaatcc
     6841 gcgttgaact gcaggcactg accaccagcg acttcgagcg tattctgacc gagccgaatg
     6901 cctctatcac cgtgcagtac aaagcactga tggcgactga aggcgtaaat atcgagttta
     6961 ccgactccgg tattaaacgc atcgcggaag cggcatggca ggtgaacgaa tctaccgaaa
     7021 acatcggtgc tcgtcgttta cacactgttc tggagcgttt aatggaagag atttcctacg
     7081 acgccagcga tttaagcggt caaaatatca ctattgacgc agattatgtg agcaaacatc
     7141 tggatgcgtt ggtggcagat gaagatctga gccgttttat cctataatcg cgttcaatca
     7201 ttttcatcat tgtttgatgg ggctgaaagg ccccattttt attggcgcgt attatgactg
     7261 aacaacaaat tagccgaact caggcgtggc tggaaagttt acgacctaaa accctccccc
     7321 tcgcctttgc tgcaattatc gtcgggacgg cgctggcatg gtggcaaggt cacttcgatc
     7381 cgctggtcgc cctgctggca ctaattaccg ccgggctatt acagatcctt tctaacctcg
     7441 ccaatgatta cggcgatgcg gtaaaaggca gcgataaacc tgaccgcatt gggccgctac
     7501 gcggcatgca aaaaggggtc attacccagc aagagatgaa acgggcgctc attattaccg
     7561 tcgtgctcat ctgtctctcc gggctggcac tggttgcagt ggcatgccat acgctggccg
     7621 attttgtcgg tttcctgatt cttggcgggt tgtcgatcat tgccgctatc acctacaccg
     7681 tgggcaatcg tccttatggt tatatcggtc tgggtgatat ttccgtactg gttttctttg
     7741 gctggttgag cgtcatgggg agctggtatt tacaggctca tacattgatt ccggcactga
     7801 tccttccggc gaccgcctgt ggcctgctgg caacggcagt actgaacatt aataacctgc
     7861 gtgatatcaa tagcgaccgc gaaaatggca aaaacacgct ggtggtgcgc ttaggtgaag
     7921 tgaacgcgcg tcgttatcat gcctgcctgc tgatgggctc tctggtatgt ctggcgctgt
     7981 ttaatctctt ttcgctgcat agcctgtggg gctggctgtt cctgctggcg gcaccattac
     8041 tggtgaagca agcccgttat gtgatgcggg aaatggaccc ggtggcgatg cgaccaatgc
     8101 tggaacgtac tgtcaaggga gcgttactga ctaacctgct gtttgtttta gggatattcc
     8161 taagccagtg ggcagcataa ctgacaaata tcaattaaca attgatgatt ttgccaacag
     8221 cccacatagc gcgatatact gaaaattctc gcagcaactg aatgttaagc ctatgaaata
     8281 cgatacttcc gagctttgtg acatctatca agaagatgtt aacgtcgtgg aaccgctgtt
     8341 ctccaacttt ggcggacggg cgtcgtttgg cggacaaata atcacggtaa aatgtttcga
     8401 ggacaacggg ttgctgtacg atctgctcga acagaatggc cgtggtcgtg ttcttgtcgt
     8461 cgatggcggt ggttctgttc gtcgcgcact ggtcgatgct gaactggcgc gtctggcagt
     8521 acaaaatgaa tgggaaggtc tggttattta cggcgcggtg cgtcaggtag atgacctgga
     8581 agagttggat atcggcatcc aggcgatggc ggcaattccg gttggtgccg ctggcgaagg
     8641 cattggcgaa agcgatgtcc gcgtcaattt tggcggtgtc accttcttct ccggcgacca
     8701 tctttatgcc gacaataccg ggattattct ttcagaagat ccgctggata ttgaatgata
     8761 agaaaggcac cgcaaggtgc cttttttctg cgttacctgt tggcctacac agtaaagaaa
     8821 ttacgcgaaa gatgaagcgt aatcagacct cttccatgcg acccagcagg gcctgcagac
     8881 gttcctgcca gccgttctgc tgttctttca gatggttgtt ctcacgctcc agctcttcgc
     8941 gctgatgctg ggcattttga acttcctgcg acagtgagtt gtttttttct ttcagctctt
     9001 cgatttccat ctgcaacaga gtgatggtat caatcgcctg ctgtactttt gcttccagtt
     9061 tctcaaacac ttctaatgac attgtcatac ctctcctgaa ttgcaaggcg ttgatggata
     9121 aaaaatcctc gtcccgatta ccggtgacgc cttaataaat acgagcgcac tttagttagc
     9181 tccgattgta tgaagccgcg ccatcgctgt ccagcggcac gccttgcaga ttacggtttg
     9241 ccacactttt catccttctc ctggagacat aatccacacc aatcgaaaat gttaataaat
     9301 ttgttgcgcg aatgatctaa caaacatgca tcatgtacaa tcagatggaa taaatggcgc
     9361 gataacgctc attttatgac gaagcacaca cattttaagt tcgatatttc tcgtttttgc
     9421 tcgttaacga taagtttaca gcatgcctac aagcatcgtg gaggtccgtg actttcacgc
     9481 atacaacaaa cattaactct tcaggatccg attatgagtc aaacatcaac cttgaaaggc
     9541 cagtgcattg ctgaattcct cggtaccggg ttgttgattt ttttcggtgt gggttgcgtt
     9601 gcagcactaa aagtcgctgg tgcgtctttt ggtcagtggg aaatcagtgt catttgggga
     9661 ctgggggtgg caatggctat ctacctgacc gcaggggttt ccggcgcgca tcttaatccc
     9721 gctgttacca ttgcattgtg gctgtttgcc tgtttcgaca agcgcaaagt tattcctttt
     9781 atcgtttcac aagttgccgg cgctttctgc gctgcggctt tagtttacgg gctttactac
     9841 aatttatttt tcgacttcga gcagactcat cacattgttc gcggcagcgt tgaaagtgtt
     9901 gatctggctg gcactttctc tacttaccct aatcctcata tcaattttgt gcaggctttc
     9961 gcagttgaga tggtgattac cgctattctg atggggctga tcctggcgtt aacggacgat
    10021 ggcaacggtg taccacgcgg ccctttggct cccttgctga ttggtctact gattgcggtc
    10081 attggcgcat ctatgggccc attgacgggt tttgccatga acccagcgcg tgactttggt
    10141 ccgaaagtct ttgcctggct ggcgggctgg ggtaatgtcg cctttaccgg cggcagagac
    10201 attccttact tcctggtgcc gctttttggc cctatcgttg gcgcgattgt aggtgcattt
    10261 gcctaccgca aactgattgg tcgccatttg ccttgcgata tctgtgttgt ggaagaaaag
    10321 gaaaccacaa ctccttcaga acaaaaagct tcgctgtaat atgactacgg gacaattaaa
    10381 catgactgaa aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt
    10441 cgtaatggat cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta
    10501 cccaaaacca ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac
    10561 gctggtagaa gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat
    10621 tacgaaccag cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa
    10681 cgccattgtc tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg
    10741 tttagaagat tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac
    10801 caaagtgaag tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga
    10861 attgctgttt ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca
    10921 tgtgaccgat tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg
    10981 ggacgacaaa atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg
    11041 ttcttccgaa gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat
    11101 ctccgggatc gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg
    11161 gatggcgaag aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc
    11221 ggtgaaatca gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa
    11281 ctatgcgttg gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga
    11341 gatgaagttg attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac
    11401 caatggtgtg tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta
    11461 tgcgcgcggg gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc
    11521 gacgctggag tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc
    11581 tggtatccgt ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat
    11641 gcagttccag tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac
    11701 cgcattgggt gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga
    11761 gctgcaagag aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg
    11821 taattaccgt tacgcaggct ggaaaaaagc ggttaaacgc gcgatggcgt gggaagaaca
    11881 cgacgaataa tgtaaatgcc gaatgaagcg tttatgccgc atccggtagt cccgaaacgt
    11941 gcgggggcaa ccccgcacac atcaataatc cctcccttcc cctgtgctac acttcgcgcc
    12001 attccttact gcttagagtt tgctatgaga cgagaacttg ccatcgaatt ttcccgcgtc
    12061 accgaatcag cggcgctggc tggctacaaa tggttaggac gcggcgataa aaacaccgcg
    12121 gacggcgcgg cggtaaacgc catgcgtatt atgctcaacc aggtcaacat tgacggcacc
    12181 atcgtcattg gtgaaggtga aatcgacgaa gcaccgatgc tctacattgg tgaaaaagtc
    12241 ggtactggtc gcggcgacgc ggtagatatt gctgttgatc cgattgaagg cacgcgcatg
    12301 acggcgatgg gccaggctaa cgcgctggcg gtgctggcag taggcgataa aggctgcttc
    12361 ctcaatgcgc cggatatgta tatggagaag ctgattgtcg ggccgggagc caaaggcacc
    12421 attgatctga acctgccgct ggcggataac ctgcgcaatg tagcggcggc gctcggcaaa
    12481 ccgttgagcg aactgacggt aacgattctg gctaaaccac gccacgatgc cgttatcgct
    12541 gaaatgcagc aactcggcgt acgcgtattt gctattccgg acggcgacgt tgcggcctca
    12601 attctcacct gtatgccaga cagcgaagtt gacgtgctgt acggtattgg tggcgcgccg
    12661 gaaggcgtgg tttctgcggc ggtgatccgc gcattagatg gcgacatgaa cggtcgtctg
    12721 ctggcgcgtc atgacgtcaa aggcgacaac gaagagaatc gtcgcattgg cgagcaggag
    12781 ctggcacgct gcaaagcgat gggcatcgaa gccggtaaag tattgcgcct gggcgatatg
    12841 gcgcgcagcg ataacgtcat cttctctgcc accggtatta ccaaaggcga tctgctggaa
    12901 ggcattagcc gcaaaggcaa tatcgcgact accgaaacgc tgctgatccg cggcaagtca
    12961 cgcaccattc gccgcattca gtccatccac tatctggatc gcaaagaccc ggaaatgcag
    13021 gtgcacatcc tctgattgat ttgatcgatt gagccttcca gtccttcggg actggaattt
    13081 ttttgttcgg agaacgaaga taaggcaagt taatcaaaac aggagaaaaa catggctgat
    13141 tgggtaacag gcaaagtcac taaagtgcag aactggaccg acgccctgtt tagtctcacc
    13201 gttcacgccc ccgtgcttcc gtttaccgcc gggcaattca ccaagcttgg ccttgaaatc
    13261 gacggcgaac gcgtccagcg cgcctactcc tatgttaact cgcccgataa tcccgatctg
    13321 gagttttacc tggtcaccgt ccccgatggc aaattaagcc cacgattggc ggcactgaaa
    13381 ccaggcgatg aagtgcaggt ggttagcgaa gcagctggct tctttgttct ggatgaagta
    13441 ccggactgcg aaacgctatg gatgctggca accggtacag cgattggccc ttatttatcg
    13501 attctgcaac taggcaaaga tttagatcgc ttcaaaaatc tggtcctggt tcacgccgca
    13561 cgttatgccg ccgacttaag ctatttgcca ctgatgcaag aactggaaaa acgctacgaa
    13621 ggaaaactgc gcattcagac ggtggtcagt cgggaaacgg cagcggggtc gctcaccggg
    13681 cggataccgg cgttaattga aagtggggaa ctggaaagcg cgattggcct gccgatgaat
    13741 aaagaaacca gccatgtgat gctgtgcggc aatccacaga tggtgcgcga tacacaacag
    13801 ttgctgaaag agacccggca gatgacgaaa catttacgtc gccgaccggg ccatatgaca
    13861 gcggagcatt actggtaagc ggttacttat cgataaacgg cacgatgagc aaatccgcac
    13921 tcatcttatt gatcatcccg cgatatgccg gcatcaaacg gttgataaat gagtgatgat
    13981 gaccacagac aaggaggtcg cactgctctt tttgcataat ttccagcagt gtttccggca
    14041 tttctccgcg ttcaatacgc agttttgtct tcggccattg aatatttttc gtcagtttat
    14101 acagcttgtt atccgactta ttcttcaaca attgaagaat atcttctgtt gcagggaagt
    14161 agatacccgg atacaactcg ctcaagccat catcaatatg aattaacgtc aggtgagcgt
    14221 cattatgtct ggcgagctcc agagctttat tcaccagtaa ggcatcttct tcattcccgg
    14281 aaattgccac gccaatgtgt ttataagcca tgtttaactc cttctaaagc aactccatca
    14341 agctaacgaa cgcagtgata actcaaaaat aatcatctcg gcctggcatg agaaagtgaa
    14401 ggccgcatca agctcaactt gcccctccag atccttaatc ttgtaggcca cgctggcccc
    14461 ttcatctgca gaggatgcag cggcggcaag ctctttcagt ttggtcataa aagactctgc
    14521 ttcttcgcgg gtggcaaaaa agcgcgaaaa cttactggta cagttgtcgt tatcaatcac
    14581 cgtaccgata tcaatcgcac atcctttagt actgcattta tctacgacat ctttcatagg
    14641 gcacctctgt atattcgcct ctctgtcgat tacgacttaa gaataatgcg ccgttaaggg
    14701 aagaacaaac aagtaatgat cttatggatt accatttttt ttgatattgc ttaaggtgaa
    14761 gagttcagaa ggattttccg ggtattgtca tttgcatcgt ggaaataaca atcccggaaa
    14821 aaaatagcag taagagatat attaaaggtt aattacttga tttattgtcg gctttatact
    14881 tcacatcctg agtatcttta ccatatttat tttcgccttg tgtgccaaca aacgcaccaa
    14941 gatcgataag catcatcacc agaatcaacg tcgggacaaa acgccccacc gcccattgcc
    15001 agacacccgg taaaatcgcc cagttacccg ccagcagcat ccacgccaca atcatcagaa
    15061 atgcccatgc gccggaacgc ccgcgatcat gcaagcgctt aacagttact gccgctgttg
    15121 gccagagcaa gcacacaagg caaaacgccg cggtctgaat atcgagtaga ttcttacccg
    15181 ccagtgagaa aagcaccagc atgcctgcga accacaggcc tatccaaatc cagaaatcac
    15241 ggcgtccaat acgcccttta aatgagaata accattgctg tatggtcatg taagttcctt
    15301 gatggttgtc ttttccagga ttctaccctt ttgacaaggg cgacagatat cgttttaatc
    15361 ggagccagtc ataacaaaag gtactgtcaa tgaagccagg gtgtacgctg ttttttctct
    15421 tatgttctgc attaaccgtt acaacaacgg cgcatgcaca aacaccagat acagcaacga
    15481 ccgcgcctta tctgctggct ggagccccta ctttcgatct ctccatcagc cagtttcgag
    15541 aagactttaa cagccagaat cctagcctgc cactgaacga atttcgtgcc atcgacagca
    15601 gtcccgacaa agccaatctc actcgtgctg caagtaaaat taacgagaac ttgtatgctt
    15661 ctacagcgct ggagcgcggt accttaaaaa tcaaaagcat tcaaatgacc tggctaccca
    15721 tccaggggcc agagcaaaaa gcagcgaaag cgaaagctca ggaatacatg gcagcagtga
    15781 tccgcacact caccccatta atgaccaaaa cacaaagcca gaaaaaactg cagtcgctgc
    15841 taacggcggg gaaaaacaaa cgttattaca ccgagacaga aggtgcactg cgttatgttg
    15901 tcgcggacaa cggcgaaaag gggctgacct tcgctgttga accgattaag ctggcgctat
    15961 ctgaatcgct tgaaggtttg aataaatgac aaaaagcaaa gcctttgtgc cgatgaatct
    16021 ctatactgtt tcacagacct gctgccctgc ggggcggcca tcttccttta ttcgcttata
    16081 agcgtggaga attaaaatgc gacatccttt agtgatgggt aactggaaac tgaacggcag
    16141 ccgccacatg gttcacgagc tggtttctaa cctgcgtaaa gagctggcag gtgttgctgg
    16201 ctgtgcggtt gcaatcgcac caccggaaat gtacatcgat atggcgaagc gcgaagctga
    16261 aggcagccac atcatgctgg gtgcgcaaaa cgtggacctg aacctgtccg gcgcattcac
    16321 cggtgaaact tctgccgcta tgctgaaaga catcggcgct cagtacatca tcatcggcca
    16381 ctctgaacgt cgtacttacc acaaagagtc tgacgaactg atcgcgaaaa aattcgcggt
    16441 gctgaaagag cagggcctga ctccggttct gtgcatcggt gaaaccgaag ctgaaaacga
    16501 agcgggcaaa actgaagaag tttgcgcacg tcagatcgac gcggtactga aaactcaggg
    16561 tgctgcggca ttcgaaggtg cggttatcgc ttacgaacct gtatgggcaa tcggtactgg
    16621 caaatctgca actccggctc aggcacaggc tgttcacaaa ttcatccgtg accacatcgc
    16681 taaagttgac gctaacatcg ctgaacaagt gatcattcag tacggcggct ctgtaaacgc
    16741 gtctaacgct gcagaactgt ttgctcagcc agatatcgac ggcgcgctgg ttggcggtgc
    16801 ttctctgaaa gctgacgctt tcgcagtgat cgttaaagct gcagaagcgg ctaaacaggc
    16861 ttaagtctga caggtgccgg atttcatatc cggcacttac tttccttaac tcttcgcctt
    16921 aacgcaaaat ctcacactga tgatcctgaa tttcctcggc tgaagcacgg ttaagcgtca
    16981 gtagatttcg ttgtgtcgcc agcaatacaa atgagttatc actctgccgt accatcgcca
    17041 gcccgtagct tcccatatgt tcccgcgcct caggtacttc ttctgccagc atcataaatg
    17101 ggctgcgttg taccagttcg ctttccgtta cccgacgcgc caggtattca tgcccgcgca
    17161 aaccacctgg cagtggcaac cagcggctgc tgatgttcgc cagattgtta tccagttgtt
    17221 tgcgcacatc aggacgaata caagagatat gaatatgaaa atggttttgc gtacgcccgg
    17281 tgcgggagtt gatcgccaaa gaaaccgcgc gatcgggaac cggctggccg tattttttgc
    17341 tcataaaatc acgcgcctgc caggccaacc aaaagaagtt cggcgttgaa ggatcggtca
    17401 acaaaggact ttcagtaccg ttaatacgat acgttggcat caacagatat tgcagtgggc
    17461 cattaagatc ttttaaaacc acgtatccgg cattgggttt gacttccgca catggcgaag
    17521 gattttgatt ttgctgctga ttgggcaaac attcctcaag gacaatctta cgtaatgtat
    17581 ccgactcttc accggttaat ttccagtaac caataccggc agccacaacg gcgataacta
    17641 tcatcaccaa aaaaagaaga cccgcttttt tcatcttttt ttccctgtac ctcaaagaga
    17701 tgcaagggta acgcaaaatc gtgacaaata aaaaacccgg tcgcgagaaa ccaccgggtt
    17761 tgataattat cctggagaaa tcagcgtttg ctgatctgat cgaacgtacc gccgttagca
    17821 aaatgctctt tttgcgcttt cgtccagccg ccgaactctt catcaatggt gaataacttc
    17881 agctttggaa acgcattttc gtactttttc gctacttcag cgtcgcgcgg acggtagtag
    17941 tttttcgcgg caatttcctg accttctggc gagtagagat atttcaggta ggcttccgcc
    18001 acctctttgg tgcctttttt ctcgaccact ttatcgacca ccgacacggt tggctctgcg
    18061 aggatagact cactcggcgt gacgatttcg aatttatctt tccccagttc attcgctgcc
    18121 agcagagctt cgttttccca agcgatcagt acatcgccaa tcccgcgctc gacaaaggtg
    18181 ttagtggagc cgcgcgcgcc agaatccaga acttcgacgt ttttatacag tgcccgaaca
    18241 aaatcctgtg cttttgcctg atcgttgttg ttgtgatgca gcgcgtagcc ccaggcagcc
    18301 aggtagttcc agcgtgcgcc accagagctt ttcggattag gcgtgatcac cgaaacaccc
    18361 ggtttaatca gatcgttcca gtcatggatc tgcttcggat tgcccttacg caccaggaaa
    18421 acaatggtgg aagtgtacgg tgcggagtta tccggcagac gtttgatcca ctctttatca
    18481 atccgcccgc gttccgcaat cgcgtccacg tcataggcca gagccagcgt gacaacatca
    18541 gcttcaatac cgttgattac cgacgtcgct tgtttacctg aaccaccgtg tgactgacga
    18601 atcaccacgt tatcaccagt ttgctgtttc cagtgggcgc tgaatgcctt gttgtactgt
    18661 tcgtacaatt cgcgcgttgg atcatatgaa acgttaagaa gctgaatatc ctttgccata
    18721 acgttggttg ccgccagcaa aaatgttaac cctacgcccc acttgttcat cgcccgactc
    18781 tcttatgttg tgttgtgatg agcaaagcgt gccagaaggt taaccaaaca ttaaagaata
    18841 aaaaaagatt ggctataact tgcgggtata tgttgggggt taaaaaggcg gaagaaatcc
    18901 gcctcatatt gctgacaaag tgcgctttat cgatgccgga tgcggcgcga acgccttatc
    18961 cggcctacaa aaatttgtaa attcaataaa ttgcagaatt cacgtaggcc tgataagcga
    19021 agcgcatcag gcatttttgc ttctgtcatc ggtttcaggc taaaggaatc tgcctttttc
    19081 cgaaatcatt aatacagttt tttcgcgcag tccagccagt cgcctttgaa cggacgcttc
    19141 atgttttcga tagcgtcgat gatgtcgtgg tgaaccagct gttcgttctg gatacctacg
    19201 caacgaccgc cgtaacctgc cagcagcaga tcgatagcgt aagcgcccat acgggaagcc
    19261 agaatacggt cgtaaggcac cggagaacca ccgcgctgga tgtggcccag cacagttgcg
    19321 cgggtttcac gaccggtttc tttctcgatg aaatgcgcca gttcgtcaac atcacacata
    19381 tgttcggtaa tcgccacgat cgcgtgtttt ttacctttcg cgatacccgc tttgatttcg
    19441 tttaccagat cttcacggct gaattcaact tccggaacca caacgaattc acagccaccg
    19501 gcaatggccg cagccaacgt cagatcgcca caataacggc ccatcacttc caccacggaa
    19561 atacgctggt gagaagaaga ggtgtcacgc agacggtcga tcgcttctac aacggtgctc
    19621 agcgcagtga agaaaccgat agtgtagtca gtgcctttga tgtcgttgtc gatagtgcca
    19681 ggcagaccga tgcacgggaa gcccatttcg gtcagacgca ttgcacccat gtaggaaccg
    19741 tcaccgccga taaccaccag cgcgtcgata ccacgttttt tcaggttttc gatagccacg
    19801 gcgcggatgt tctcgtcgcg gaattccggg aaacgcgcag aaccgaggaa cgtaccacca
    19861 cggttgatca tgtcagaaac gctgtaacgg tctagctgta ccatacggtc ttcatacaga
    19921 cccagatagc cgtcataaat gcccattact tccagacctt ctgtcagcgc agaacgaaca
    19981 accccgcgaa ttgcggcgtt catgcctggc gcatcaccgc cgcttgtcaa cacaccgatt
    20041 ttcttaatca tgactacctc tgaactttgg aatgcaaaat aaaatctgtt gccggaagtc
    20101 ttcttgcaca tcgaagtgat ccaacgaatg tgcaaatagt ataacaatca cttcctgctg
    20161 aattgattca ggtcaggcca aatggcggta ttttatacac aaaatgcggg tctggctctc
    20221 ttttatactg attatgaaag catagaccgt ttaccctccc tgggtacgac ggaacagggg
    20281 tcctgatgga taattacatc cgatcccgga aaacgccgta aaatagcctg ctctacctga
    20341 tccgccacca tatgtgcctg aaccaaaggc agagagtctt ccatttccaa atgaatctga
    20401 ataaagcggg tcggccctga ctgccgcgtg cgaagatcgt gagcgccgct aacacccggc
    20461 caggaagtca cgatatcaat aatttcttgc cgttcctcat caggcaatgc gcgatccagt
    20521 aatgactgta ccgcctcata tcccatgcgt aacgcgctat ataaaatata gatgccgatt
    20581 cccaatgcaa acagagcatc ggcgcgatgc cagccgtacc aggacaaccc cagcgccagc
    20641 agaattgcgc cgttcatcat aacatcagac tggtaatgta gcatatcagc ccgcaccgcc
    20701 tggctttgcg tccggcgcac cacccaacgc tgaaacgaga caaggataat cgtacaaatt
    20761 agcgccacaa ttgtcacgat aaccccgacg cctggatctg tcatcggtgt tggagatatc
    20821 agatgttgaa tacccgtcaa aaacaggaat agtgccgaac cggagataaa catactttgc
    20881 gccagcgccg cgagggactc tgctttaccg tgaccaaacg agtgattatc gtcggcaggt
    20941 tgcagggaat atcgcaccac cagtaaattc gtcaacgacg cgccgatatc caccagcgaa
    21001 tccaccagcg cggcgagaat actcaccgac ccggtatacc accatgcaaa aattttaatc
    21061 agcaatagca acgaagccat cgccgtcgca gcaatcgccg cccgactgac cagccgtcca
    21121 taagattgat tcataaatac tcccgctatc aactgacgct agtataacgg aagcaaatca
    21181 tctgcaatgc attaagcagc aggcaaattg aggataaaaa aaacccccac atcatgtggg
    21241 ggaagacagg gatggtgtct atggcaagga aaacagggtt tactactggg aacgtgagtt
    21301 gctactactc aatagcttca acgatgaact tttttgccat tgcgtcacgt cacgcaactg
    21361 ctccattcgt tgttgatgtt tctcgtttaa aaccgcttgc tgctccggcg ttaacaggcg
    21421 atacatttgg ttgcggactt ttgccatctc aacctgacga gcaatttgct cattcgccat
    21481 tttttctgcc tgtgcgcgca cagcgttttc atcaaaattt tctgcggtga caaggcgatg
    21541 cattgtctcc agttcgctaa cattaacagg aggctgttcg tgccgggcct gttgcataag
    21601 atctcgcatc tgctgacgct gatgttcggt taaacttatg ccgtcgaaca tatggctctg
    21661 cgtactgcgc tgcgtaagtt cttcacccgg atgccagtta tcgcctgaac cgacttcagc
    21721 agcgtggctt aatgaactga ctgccagcgt tgaggccatg acggcagcgg taactatgcg
    21781 catcatttgc tcccaaaatc tttctgtcgc gattcaacga tagagagttt acgattcagg
    21841 ctgcaaacat gcgtcagggg gtgtaaaaca acgtaaagtc atggattagc gacgtctgat
    21901 gacgtaattt ctgcctcgga ggtatttaaa caatgaataa aatcctgtta gttgatgatg
    21961 accgagagct gacttcccta ttaaaggagc tgctcgagat ggaaggcttc aacgtgattg
    22021 ttgcccacga tggggaacag gcgcttgatc ttctggacga cagcattgat ttacttttgc
    22081 ttgacgtaat gatgccgaag aaaaatggta tcgacacatt aaaagcactt cgccagacac
    22141 accagacgcc tgtcattatg ttgacggcgc gcggcagtga acttgatcgc gttctcggcc
    22201 ttgagctggg cgcagatgac tatctcccga aaccgtttaa tgatcgtgag ctggtggcac
    22261 gtattcgcgc gatcctgcgc cgttcgcact ggagcgagca acagcaaaac aacgacaacg
    22321 gttcaccgac actggaagtt gatgccttag tgctgaatcc aggccgtcag gaagccagct
    22381 tcgacggaca aacgctggag ttaaccggca ctgagtttac cctgctctat ttgctggcac
    22441 agcatctggg tcaggtggtt tcccgtgaac atttaagcca ggaagtgctg ggcaaacgcc
    22501 tgacgccttt cgaccgcgct atcgatatgc acatttccaa cctgcgtcgt aaactgccgg
    22561 atcgtaaaga tggtcacccg tggtttaaaa ccttgcgtgg tcgcggctat ctgatggttt
    22621 ctgcttcatg ataggcagct taaccgcgcg catcttcgcc atcttctggc tgacgctggc
    22681 gctggtgttg atgttggttt tgatgttacc caagctcgat tcacgccaga tgaccgagct
    22741 tctggatagc gaacagcgtc aggggctgat gattgagcag catgtcgaag cggagctggc
    22801 gaacgatccg cccaacgatt taatgtggtg gcggcgtctg ttccgggcga ttgataagtg
    22861 ggcaccgcca ggacagcgtt tgttattggt gaccaccgaa ggccgcgtga tcggcgctga
    22921 acgcagcgaa atgcagatca ttcgtaactt tattggtcag gccgataacg ccgatcatcc
    22981 gcagaagaaa aagtatggcc gcgtggaact ggtcggtccg ttctccgtac gtgatggcga
    23041 agataattac caactttatc tgattcgtcc ggccagcagt tctcaatccg atttcattaa
    23101 cttactgttt gaccgcccgt tattactgct gattgtcacc atgttggtca gtacgccgct
    23161 gctgttgtgg ttggcctgga gtctggcaaa accggcgcgt aagctgaaaa acgctgccga
    23221 tgaagttgcc cagggaaact tacgccagca cccggaactg gaagcggggc cacaggaatt
    23281 ccttgccgca ggtgccagtt ttaaccagat ggtcaccgcg ctggagcgca tgatgacctc
    23341 tcagcagcgt ctgctttctg atatctctca cgagctgcgc accccgctga cgcgtctgca
    23401 actgggtacg gcgttactgc gccgtcgtag cggtgaaagc aaggaactgg agcgtattga
    23461 aaccgaagcg caacgtctgg acagcatgat caacgatctg ttggtgatgt cccgtaatca
    23521 gcaaaaaaac gcgctggtta gcgaaaccat caaagccaac cagttgtgga gtgaagtgct
    23581 ggataacgcg gcgttcgaag ccgagcaaat gggcaagtcg ttgacagtta acttcccgcc
    23641 tgggccgtgg ccgctgtacg gcaatccgaa cgccctggaa agtgcgctgg aaaacattgt
    23701 tcgtaatgct ctgcgttatt cccatacgaa gattgaagtg ggctttgcgg tagataaaga
    23761 cggtatcacc attacggtgg acgacgatgg tcctggcgtt agcccggaag atcgcgaaca
    23821 gattttccgt ccgttctatc ggaccgatga agcacgcgat cgtgaatctg gcggtacagg
    23881 attggggctg gcgattgttg aaaccgccat tcagcagcat cgtggctggg tgaaggcaga
    23941 agacagcccg ctgggcggtt tacggctggt gatttggttg ccgctgtata agcggagtta
    24001 aactccgcat ttgtaggcag gataaggcgt ttacgccgca tccggcattt gagcaggatg
    24061 cctgatgcga cgctgatagc gtcttatcag gcctacactc cgggggttat tttccccaca
    24121 accggcggga aaaatcttcg atcttgccgc tcagtcggcg cttttgcatc gtccgcgtcc
    24181 agcttttcga taacccggcg gcggagagta aacggtgata ctgatcgtca tcaaacggca
    24241 tatgccatgc gatggcggca gcctcctgca cggtcacatc gctgacacgg gaaaccaact
    24301 ccagcggcgc gtccgcagac actttccctg gcgctatcac gctatacaac caccccacct
    24361 tacctgtgtt ttgcatcaac tgcgctatat cgctgatatc aaaatggtaa ttgagtttat
    24421 agcaaggcga gcgcggctgg ctgacctgaa ttaatgcctc tccccagcgg aaaatatcgc
    24481 ccatatgaac attactttcc gtgagaccgt cggttgagag attttcgcca aacgcaggcg
    24541 caacaaacaa ctccgcctgt tccggaaatt cccgcaccca gtagagatag tgctcacgag
    24601 gataatgaca cagcgctctg tctggcccgc cgtgaacctt tttctccgcc tgctcgtcac
    24661 cttccagccc cagctccgtc agcatcaact caccatcaac ctggattttg gcaattgcgc
    24721 tgggtttgcc ttcgggataa gcctgaatct tgcctgtgta tacatcaacc ggatatcgca
    24781 tctgttccct ctccacgaga aatttcccca ttagaccata tttattttat gtcaggcgtg
    24841 ctgttttcgc ggtaagtatc caggcaggaa ctcaatagca aaccggacga acgttagttc
    24901 atccggctgt ggggattaag gaagcaggtt tatttcaccg tacctaaaat ttcgattttc
    24961 gctgctctgg ctttgacttt acgtgaccag atagaggtaa taattggcac cagaatcgaa
    25021 gtcacaatga ccgccgtcgc taccagcgaa gttgctgccg gagccatcgg tttaaacgca
    25081 ggcaccattt ctgcaatcag cacaggtgtc gctaccgcgg cccctgcgga actggaagcg
    25141 gcaattccgg cagtgccatc gccaccgcca atcaatttat cggcgataat caacggaata
    25201 ccggtcacga taattactgc cacacccaac aggatcccca gcaaaccagt ctgggcaatt
    25261 acagtcaaat caatggtatt gcccagtgcg aaggcaaaga atggaatcag cgtctgcacc
    25321 gctttgctga aaaattctcg taattcaggg tcaaggttcc ccagggcaaa gcccaccagg
    25381 aacggtaata ctgcgccgac gaaaacatgc ggttcaaacg aggcaatccc tgcagtgccc
    25441 agaataatca tcgtcatgag cggcccggac tccaacgaca tcaacacaaa tgccccagct
    25501 tcttcttttg tgccgtactg ctgcatgatg gaagcgtaaa gtccgccatt ggtcatatcc
    25561 atcgccgcca ccagcgccag cgttgaaagt ccggcaaaga atccaacttc aacaccatgt
    25621 tccggaataa tgcgcgaggc aattgccgca accacccacg cgacggcaat ttttgttacc
    25681 accagcgtac cggatttacg caataccgtt cccgtcgcgc ttaattttat tgacgccccc
    25741 atgcaaaaaa accacaccgc cagaatgggc accgtaccgg taatcatccc gttggtgaat
    25801 gatccaaaat atttccccgc ccccggcgag aaggtgtggc acagtgcgcc aaggaatagc
    25861 gggacgagca tcatcccccc cgggattttc tcaatcgagc gttttatctg catttccatc
    25921 acctgtcggc atatttttaa gggctgatgc gtcatttctc aatggagcat cagtgttgat
    25981 ggaaacgaag atagacattc atttaatcaa taaaagtgat caaaaccaca tcaattgaaa
    26041 cactgtttta tttttatcgg atcattgttt ttattttaaa attgatcaca aaaaaaccac
    26101 cgccgttggc gatggttcat tttttaagct gatatgcggc gtaaacgcct cattgcagct
    26161 ggcggcaaat gattattttt tcgccgcgaa acgcgccgca gcttcgtccc agttcaccac
    26221 gttccagaac tctttgatgt agtccgggcg gcggttctgg aatttcaggt agtaagcatg
    26281 ttcccacaca tccaggccca gaatcgggaa gccggaagcg ccagaaatag cttcacccat
    26341 cagcggagaa tcctggttag cagtagaaac caccgccagt ttatcgcctt tcagcaccag
    26401 ccatgcccag ccggaaccaa agcgggaagc tgccgctttt tcaaattctg ctttgaagtt
    26461 atcaacggag ccgaagtcac gttcgatagc cgctttcagg tcaccctgca gggtggtgcc
    26521 ttttttcaga cctttccaga acaggctgtg gttagcgtga ccgccagcgt tgttgcgcag
    26581 tacggttttc ttgtctgctg gcagctggtc cagtttggtg atcagctctt caaccggcag
    26641 gttggcaaat tctggcaggc tttccagcgc cgcgttggcg ttgtttacgt aggtctgatg
    26701 gtgtttggtg tggtggattt ccatggtctg cttatcgaag tgcggttcca gggcatcgta
    26761 agcatacggc agggatggca gggtatagct catattcatc tccagtattg tcgggcggcc
    26821 gattgttaat gccgcgtaag cagttggttc attatagtta attaaatgat attgaaaatg
    26881 attatcaatg ccgtactttt cgtaagggta tggttttgca ggaaaatgcc cgagatgtga
    26941 agcaaatcac ccacttaatg ccgtgattgc cagtaaatcg acaacggcgg caacaggcga
    27001 aaggttaatc gacagcacga tttttacact catctcgtcg gagatgtgaa gcgacgaaaa
    27061 atgatgagga taagaagatg agtaacgcga ttacgatggg gatattttgg catttgatcg
    27121 gcgcggccag tgcagcctgt ttttacgctc cgttcaaaaa agtaaaaaaa tggtcatggg
    27181 aaaccatgtg gtcagtcggt gggattgttt cgtggattat tctgccgtgg gccatcagcg
    27241 ccctgttact accgaatttc tgggcgtatt acagctcgtt tagtctctct acgctgctgc
    27301 ctgtttttct gttcggcgct atgtggggga tcggtaatat caactatggc ctgaccatgc
    27361 gttatctcgg catgtcgatg ggaattggca tcgccattgg cattacgttg attgtcggta
    27421 cgctgatgac gccaattatc aacggcaatt tcgatgtgct gattaacacc gaaggcggac
    27481 gcatgacgtt gctcggcgtt ctggtggcgc tgattggcgt agggattgta actcgagccg
    27541 ggcagttgaa agagcgcaag atgggcatta aagccgaaga gttcaatctg aaaaaagggc
    27601 tggtgctggc ggtgatgtgc ggcattttct ctgccgggat gtcctttgcg atgaacgccg
    27661 caaaaccgat gcatgaagcc gctgccgcac taggcgtcga tccactgtat gtcgctctgc
    27721 caagctatgt catcatcatg ggcggcggcg cgatcatcaa cctcggtttc tgttttattc
    27781 gtctggcaaa agtgaaggat ttgtcgctaa aagccgactt ctcgctggca aaaccgctga
    27841 tcactcacaa tgtgttactc tcagcactgg gcgggttgat gtggtatctg caattctttt
    27901 tctatgcctg gggccacgcc cgcattccgg cgcaatatga ctacatcagt tggatgctgc
    27961 atatgagctt ctatgtattg tgcggcggta tcgtcgggct ggtgctgaaa gagtggaaca
    28021 atgcaggacg ccgtccggta acggtgttga gcctcggttg tgtggtgatt attgtcgccg
    28081 ctaacatcgt cggcatcggc atggcgaatt aatctttctg cgaattgaga tgacgccact
    28141 ggctgggcgt catcccggtt tcccgggtaa acaccaccga aaaatagtta ctatcttcaa
    28201 agccacattc ggtcgaaata tcactgatta acaggcggct atgctggaga agatattgcg
    28261 catgacacac tctgacctgc cgcagatatt gattgatggt cattccagtc tgctggcgaa
    28321 attgctgacg caaaacgcgc tcactgcacg atgcctcatc acaaaattta tccagcgcaa
    28381 agggactttt caggctagcc gccagccggg taatcagctt atccagcaac gtttcgctgg
    28441 atgttggcgg caacgaatca ctggtgtaac gatggcgatt cagcaacatc accaactgcc
    28501 cgaacagcaa ctcagccatt tcgttagcaa acgacacatg ctgactactt tcatgctcaa
    28561 gctgaccgat aacctgccgc gcctgcgcca tccccacgct acctaagcgc cagtgtggtt
    28621 gccctgcgct ggcgctaaat cccggaatcg ctccctgcca gtcaagattc agcttcagcc
    28681 gctccgggca ataaataata ttctgcaaaa ccagatcgtt aacggaagcg taggagtgtt
    28741 tatcgtcagc atgaatgtaa aagagatcgc cacgggtaat gcgataaggg cgatcgttga
    28801 gtacatgcag accattaccg cgccagacaa tcaccagctc acaaaaatca tgtgtatgtt
    28861 cggcaaaaac atcttgcgga taacggtcag ccacagcgac tgcctgctgg tcgctggcaa
    28921 aaaaatcatc tttgagaagt tttaactgat gcgccaccgt ggctacctcg gccagagaac
    28981 gaagttgatt attcgcaata tggcgtacaa atacgttgag aagattcgcg ttattgcaga
    29041 aaaccatccc gtccctgacg aatatcacgc ggtgaccagt taaactctcg gcgaaaaagc
    29101 gtcgaaaagt ggttactgtc gctgaatcca cagcgatagg cgatatcagt aacgctggcc
    29161 tcgctgtggc gtagtagatg tcgggctttc atcagtcgca ggcggttcag gtaacgctga
    29221 ggcgtcagtc ccgtttgctg cttaagctgc cgatgtagcg tacgcagcga aagagaaaat
    29281 tgctccgcca cggcatccca attcacctca tcggcaaaat ggtcctccag ccaggccaga
    29341 agcaagttga gacgtgatgc gctgttttcc aggttctcct gcaaactgct tttacgcagc
    29401 aagagcagta attgcataaa caagatctcg cgactggcgg tcgagggtaa atcattttcc
    29461 ccttcctgct gttccatctg tgcaaccagc tgtcgcacct gctgcaatac gctgtggtta
    29521 acgcgccagt gggaaggata ctgcccatcc agctcttgtg gcagcaactg attcagcccg
    29581 gcgagaaact gaaatcgatc cggcgagcga tacagcacat tagtcagaca cagattatcg
    29641 gtatgttcat acagatgccg atcatggtcg cgcacaaaac agaccgtgcc accggtgatg
    29701 gtatagggct gcccattaaa cacatgaata cccgtgccat gttcgacaat cacaatttca
    29761 tgaaaatcat gatgatgttc aggaaaatcc gcctgcggga gccggggttc tatcgccacg
    29821 gacgcgttac cagacggaaa aaaatccaca ctatgtaata cggtcatact ggcctcctga
    29881 tgtcgtcaac acggcgaaat agtaatcacg aggtcaggtt cttaccttaa attttcgacg
    29941 gaaaaccacg taaaaaacgt cgatttttca agatacagcg tgaattttca ggaaatgcgg
    30001 tgagcatcac atcaccacaa ttcagcaaat tgtgaacatc atcacgttca tctttccctg
    30061 gttgccaatg gcccattttc ctgtcagtaa cgagaaggtc gcgaattcag gcgcttttta
    30121 gactggtcgt aatgaaattc agcaggatca cattatgacc tttcgcaatt gtgtcgccgt
    30181 cgatctcggc gcatccagtg ggcgcgtgat gctggcgcgt tacgagcgtg aatgccgcag
    30241 cctgacgctg cgcgaaatcc atcgttttaa caatgggctg catagtcaga acggctatgt
    30301 cacctgggat gtggatagcc tggaaagtgc cattcgcctt ggattaaaca aggtgtgcga
    30361 ggaagggatt cgtatcgata gcattgggat tgatacctgg ggcgtggact ttgtgctgct
    30421 cgaccaacag ggtcagcgtg tgggcctgcc cgttgcttat cgcgatagcc gcaccaatgg
    30481 cctgatggcg caggcacaac aacaactcgg caaacgcgat atttatcaac gtagcggcat
    30541 ccagtttctg cccttcaata cgctttatca gttacgggcg ctgacggagc aacaacctga
    30601 acttattcca cacattgctc acgctctgct aatgccggat tattttagtt atcgccttac
    30661 cggcaagatg aactgggagt acaccaacgc cacaaccacg caactggtca atatcaatag
    30721 cgacgactgg gacgagtcgc tactggcgtg gagcggtgcc aacaaagcct ggtttggtcg
    30781 cccgacgcat ccgggtaatg tcataggtca ctggatttgc ccgcagggta atgagattcc
    30841 ggtggtcgcc gttgccagcc atgataccgc cagcgcggtt atcgcctcgc cgttaaacgg
    30901 ttcacgcgcc gcttatctct cttctggtac ctggtcattg atgggcttcg aaagccagac
    30961 gccatttacc aatgacacgg cgctggcagc caacatcacc aatgaaggcg gggcggaagg
    31021 tcgctatcgg gtgctgaaaa atattatggg cttatggctg cttcagcggg tgcttaagga
    31081 gcggaaaatc aacgatctcc cggcgcttat cgtcgcgaca cagtcacttc cggcctgccg
    31141 cttcatcatc aatcccaatg acgatcgctt tattaatcct gaggcgatgt gcagcgaaat
    31201 tcaggctgcg tgtcgggaaa cggcgcaacc gatcccggaa agtgatgctg aactggcgcg
    31261 ctgcattttc gacagtctgg cgctgctgta tgccgatgtg ttgcatgagc tggcgcagct
    31321 acgcggtgaa gatttctcgc aactgcatat tgtcggcgga ggctgtcaga acacgctgct
    31381 caaccagcta tgtgccgatg cctgcggtat tcgggtgatc gccgggcctg ttgaagcctc
    31441 gacgctcggc aatatcggca tccagttaat gacgctggat gaactcaaca atgtggatga
    31501 tttccgtcag gtcgtcagca ccaccgcgaa tctgaccacc tttaccccta atcctgacag
    31561 tgaaattgcg cactacgtgg cgcggattca ctctacacga aagacaaagg agctttgcgc
    31621 atgaccactc aactggaaca ggcctgggag ctggcgaaac agcgtttcgc ggcggtgggg
    31681 attgatgtcg aggaggcgct gcgccaactt gatcgtttac ccgtttcaat gcactgctgg
    31741 cagggcgatg atgtttccgg ttttgaaaac ccggaaggtt cgctgaccgg ggggattcag
    31801 gccacaggca attatccggg caaagcgcgt aatgccagtg agctacgtgc cgatctggaa
    31861 caggctatgc ggctgattcc ggggccgaaa cggcttaatt tacatgccat ctatctggaa
    31921 tcagatacgc cagtctcgcg cgaccagatc aaaccagagc acttcaaaaa ctgggttgaa
    31981 tgggcgaaag ccaatcagct cggtctggat tttaacccct cctgcttttc gcatccgcta
    32041 agcgccgatg gctttacgct ctcgcatccc gacgacagca ttcgccagtt ctggattgat
    32101 cactgcaaag ccagccgtcg cgtttcggcc tattttggcg agcaactcgg cacaccatcg
    32161 gtgatgaaca tctggatccc ggatggtatg aaagatatca ccgttgaccg tctcgccccg
    32221 cgtcagcgtc tgctggcagc tctggatgag gtgatcagcg agaagctgga tccggcgcac
    32281 catatcgacg ccgttgagag caaattgttt ggcattggcg cagagagcta cacggttggc
    32341 tccaatgagt tttacatggg gtatgccacc agccgccaga ctgcgctatg cctggacgcc
    32401 gggcacttcc acccgactga agtgatttcc gacaagattt ccgccgccat gctgtatgtg
    32461 ccgcagttgc tgctgcacgt cagccgtccg gttcgctggg acagcgatca cgtagtgctg
    32521 ctggatgatg aaacccaggc aattgccagt gagattgtgc gtcacgatct gtttgaccgg
    32581 gtgcatatcg gccttgactt cttcgatgcc tctatcaacc gcattgccgc gtgggtcatt
    32641 ggtacacgca atatgaaaaa agccctgctg cgtgcgttgc tggaacctac cgctgagctg
    32701 cgcaagctgg aagcggcggg cgattacact gcgcgtctgg cactgctgga agagcagaaa
    32761 tcgttgccgt ggcaggcggt ctgggaaatg tattgccaac gtcacgatac gccagcaggt
    32821 agcgaatggc tggagagcgt gcgggcttat gagaaagcga ttttgagcca gcgtgggtaa
    32881 acactgccgg atgcggcgcg agcgccttat ccggcctacg ggtcgtgcat ctgacaacac
    32941 cgaatttaca ggaacacaga acatgcaaaa cattacccag tcctggtttg tccagggaat
    33001 gatcaaagcc accaccgatg cctggctgaa aggctgggac gagcgcaacg gcggcaacct
    33061 gacgctgcgc ctggatgacg ccgatatcgc gctttatcac gacaacttcc acccacaacc
    33121 gcgctatatt ccgctcagcc agcccatgcc tttactggca aatacgccgt ttattgtcac
    33181 cggctccggc aaattcttcc gtaacgtcca gcttgatcct gcagctaact taggcgtcgt
    33241 aaaagtcgac agcgacggcg cgggctacca tattctctgg gggttaacca acgaagccgt
    33301 tcccacttcc gaacttccgg ctcacttcct ttcccactgc gagcgcatta aagccaccaa
    33361 cggtaaagat cgggtgatca tgcactgcca cgccaccaac ctgatcgccc tcacctatgt
    33421 actggaaaac gacaccgcgg tctttactcg ccaactgtgg gaaggcagca ccgagtgtct
    33481 ggtagtgttc ccggatggcg ttggcatttt gccgtggatg gtgcccggca cggacgaaat
    33541 cggccaggcg accgcacaag agatgcaaaa acattcgctg gtgttgtggt ccttccacgg
    33601 cgtcttcggc agcgggccga cgctggatga aacattcggt ttaatcgaca ccgcagaaaa
    33661 atcagcacaa gtattagtga aggtttattc gatgggcggc atgaaacaga ccatcagccg
    33721 tgaagagttg atagcgctcg gcaagcgctt cggcgttaca ccactagcca gtgcgctggc
    33781 gctgtaagga gcaaacatga tccgcaaagc ctttgtcatg caggttaacc ccgacgccca
    33841 cgaagagtat cagcgtcggc ataaccccat ctggccggaa ctggaagcag tgttgaaatc
    33901 tcacggtgcg cataactacg ctatttacct cgacaaagcg cgtaatctgc tgtttgccac
    33961 ggtagagatt gaatctgaag aacggtggaa tgcggtagcc agtactgatg tttgccaacg
    34021 ttggtggaaa tatatgaccg atgttatgcc cgctaacccg gataacagcc cggtgagtag
    34081 cgagctgcaa gaagtgtttt acctgccgta attgcccaat gtgccggaga tgcctccggc
    34141 acaatatttt aaatttcgat ttgaaaaatt aaacatatta catctcccgg taagttatat
    34201 ttccctgata cattgtgagt aaatcacaaa aataatgaat aacccattaa tgattcatgt
    34261 ggtttattta aataaccatt atgtgcatta ctccgcaaat ctgacctttc actctgtctc
    34321 aaattgtcat tgtttaccga catatcgcct ctcatcactt aatcgctcca ccattacaat
    34381 tgattaaaat atattttatg gagtccgatt aatggcagct cttactgcaa gctgtattga
    34441 cctgaatatt cagggcaatg gcgcttattc cgttctgaag cagttggcga caatagcgtt
    34501 acaaaacggt tttatcaccg actcacacca gttcctgcaa accctgctgc tgcgcgaaaa
    34561 aatgcactct actggatttg gttccggtgt cgccgtgccg cacggtaaaa gcgcctgcgt
    34621 taaacaaccg ttcgtattat tcgcccgcaa agcgcaggct attgactgga aagccagcga
    34681 tggcgaagac gtcaattgct ggatctgcct cggcgtgccg caaagcggcg aagaggatca
    34741 ggtcaaaatc atcggcacac tgtgtcgcaa aattatccac caggatttta ttcatcaact
    34801 gcaacagggc gacgccgacc aggtgcttgc cttgttaaat caaaccctca gctcataagg
    34861 aagtggcgat ggagtcatcc ttacgtattg tcgcgatcac caactgcccc gccgggatcg
    34921 ctcacaccta catggtggcg gaagccctgg aacagaaagc ccgttctctc ggccatacca
    34981 taaaagtgga aactcaaggg tccagtggcg ttgaaaaccg cttatccagc gaagagattg
    35041 ccgctgccga ttacgtcatt ctcgctaccg ggcgtggcct gagcggtgat gatcgcgcgc
    35101 ggtttgccgg gaagaaagtt tatgagattg ccatctccca ggcgttgaaa aatatcgacc
    35161 agattttcag cgaattaccg acaaactcgc agctttttgc cgcagatagc ggcgtgaagc
    35221 ttggcaaaca ggaagtgcaa agcggcagcg tgatgagcca cctgatggcg ggcgtctctg
    35281 cggcgctgcc gtttgtcatc ggcggtggta tcctggtggc gcttgccaac atgctggtgc
    35341 agttcggttt gccgtatacc gatatgtcga aaggcgcgcc gtcgtttacc ttggtagttg
    35401 aatccatcgg ttaccttggc ttcaccttta tgatccccat catgggcgct tatattgcct
    35461 cgtcgattgc cgataaaccg gctttcgctc cggcgtttct ggtttgctat ctggcgaacg
    35521 acaaagcgct gctcggcacc cagtcgggtg cgggattcct cggcgcggtg gtgctggggc
    35581 tggcgattgg ctatttcgtc ttctggttcc gcaaagtgcg ccttggcaaa gcattacaac
    35641 cgctgctcgg ttcaatgctg atcccgttcg ttactctgct ggttttcggc gtgctcactt
    35701 actacgttat cgggccggtg atgtccgacc tcatgggcgg gctgctccac ttcctgaata
    35761 ccattcctcc atcaatgaag tttgcggcag cgtttctggt cggtgcgatg ctggcgttcg
    35821 atatgggcgg ccccatcaat aaaaccgcct ggttcttctg cttctcactg ctggaaaaac
    35881 acatttatga ctggtacgcc atcgtcggcg ttgtcgcgtt gatgccgccc gttgcggcgg
    35941 gtcttgcgac gttcattgcg cccaaactgt ttacccgaca ggaaaaagag gctgccagta
    36001 gtgccattgt ggtcggtgcc acagtcgcaa ccgaaccggc tataccttac gctctggcag
    36061 ctccgttgcc gatgattacc gccaatacgc tggcgggtgg cattaccggc gtgctggtga
    36121 tcgctttcgg aatcaaacgt ctggcacctg gtttgggtat ctttgacccg ctgatcggcc
    36181 tgatgtcgcc ggtgggttcg ttttatctgg tgctggcgat tggcctcgcg ctgaacattt
    36241 cattcattat cgtcctgaaa ggattgtggt tacgccgcaa agcgaaagcc gcgcaacagg
    36301 agcttgtcca tgaacattga gttactgcaa cagttgtgcg aagccagcgc cgtcagcggc
    36361 gatgaacagg aagttcgcga cattctgata aacacgctgg aaccttgcgt taatgagatc
    36421 acctttgatg gtctgggcag ctttgttgcc cgtaagggga ataaaggtcc aaaagttgcc
    36481 gttgtcgggc atatggatga agtcggcttt atggtcaccc acatcgacga gagcggtttt
    36541 ctgcgtttca ccaccattgg tggctggtgg aatcagtcga tgctcaacca ccgggtaacc
    36601 atacgcacac acaagggagt gaaaatccct ggtgtgattg gttccgtcgc gcctcatgcg
    36661 ttaacggaaa agcaaaggca acagccgctg tcatttgatg agatgttcat tgatattggc
    36721 gcgaacagtc gcgatgaggt ggaaaaacgc ggtgtggcga tgggcgattt tattagcccg
    36781 gaagccaatt ttgcctgctg gggcgaagat aaagtggtcg gcaaggcgct ggataaccgc
    36841 atcggctgcg caatgatggc tgaactattg cagacggtga ataatcccga aattacgctg
    36901 tatggcgtcg gcagcgtgga agaagaagtt gggctacgcg gggcacaaac ctcggcggaa
    36961 cacattaaac cggatgtggt gattgtgctg gataccgctg tcgcaggtga tgttccgggc
    37021 attgataaca ttaaataccc gctgaaactg ggccaggggc cggggctgat gctgtttgac
    37081 aagcgctact tccccaacca gaaactggta gcagcgttta aaaactgtgc cacgcagaat
    37141 gatttaccgc tgcaattttc caccatgaaa accggagcga cggatggcgg gcgctacaac
    37201 gtgatgggcg gcgggcgtcc ggttgtcgcg ctgtgtctgc caactcgtta tctgcacgcc
    37261 aacagcggta tgatttcaaa agccgattac gatgctctgc tcacgctgat acggggtttt
    37321 ctgacgacct taactgcgga gaaagtcaac gcgtttagcc agttccgtca ggtggattaa
    37381 tgctaaacga acgccagtta aagattgtcg atctgctgga gcaacagccg cgcacgcctg
    37441 gcgagctggc gcaacagact ggcgtttcag gcaggaccat cctgcgtgat attgactatc
    37501 tcaacttcac ccttaacggc aaagcccgca ttttcgccag tggcagtgcg ggctatcagc
    37561 tggaaatctt cgagcgccgc agcttttttc agttgctgca aaagcacgat aacgacgatc
    37621 ggctgctggc gctgttatta ctgaatactt tcactccccg tgcgcaactc gcctcggcgc
    37681 ttaatttgcc agaaacgtgg gtagcagagc gtctgccccg gttaaaacag cgttatgaac
    37741 gcacttgttg cctggccagc cgccctggtt tgggccattt cattgatgag acagaagaga
    37801 aacgcgttat cttgctggcg aacttgctgc gcaaagatcc gtttttaatt ccgctggcgg
    37861 gcataacacg agacaacctt cagcatttat ccacggcctg cgacaaccaa caccgctggc
    37921 cgctcatgca gggtgattat ctctccagcc tgattctggc gatttacgcc ctgcgtaatc
    37981 aactgaccga tgagtggccg caatatcccg gtgacgagat aaaacaaatc gttgaacata
    38041 gcggtctgtt tctcggtgat aattctgtaa gaaccctgac gggtttgata gagaaacagc
    38101 atcagcaagc gcaggtaatt tcagccgatc atgtgctggg gttgctgcaa agggtgccag
    38161 gcatcgcgtc attgaatatt attgatacgc agctggttga gaatattacc gggcatttat
    38221 tacgttgcct tgccgcaccg gtgtggattg ctgagcaccg ccaaagcagc atgaataacc
    38281 tgaaagcagc ctggccagcg gcctttgata tgagtctgca ctttattacg ctactgcgtg
    38341 aacagctcga tattcccctt ttcgacagcg atctgatcgg tttgtatttt gcctgtgcgc
    38401 tggaacggca tcaaaacgaa cgccagccga tcattttgct ctcggaccag aacgcgattg
    38461 ccactattaa tcagctcgcc attgagcgcg atgttttaca ttgtcgggtg attattgccc
    38521 gtagcttaag cgaacttgtt gccattcgcg aagagattga gccgttattg atcattaaca
    38581 acagccatta tttactggat gacgcggtaa ataattgcat caccgtaaaa aatatcatta
    38641 cggctgccgg tatcgaacaa ataaaacatt ttctggcgac ggcatttatt cgccagcaac
    38701 cggagcgttt tttctctgcc cccggaagtt ttcattattc gaatgtacgc ggtgaaagct
    38761 ggcaacatat tacccggcaa atttgtgcgc aattagtggc acaacaccat attaccgccg
    38821 atgaagcgca acggatcatc gcccgcgaag gcgaaggtga aaacctgatt gttaatcgcc
    38881 tcgccatccc acattgctgg agcgaacagg agcgacgttt tcgtggattt tttattaccc
    38941 tcgcccaacc agttgaggtg aataacgaag tcattaacca tgtcttgatc gcctgcgccg
    39001 ccgccgatgc gcgtcacgag ttgaaaatat tcagctatct ggcaagcgta ttgtgtcagc
    39061 atccggcaga ggttattgcc gggttaacag gatatgaggc atttatggag ttacttcaca
    39121 aggggtgaac tgtgtcggat gcgccgcatc cgacactgca tttatcatca aaaacgttta
    39181 acgcaggcta ttatagtcat caaccatttc gttgtactcg cgtaatgctc tcgggaaaga
    39241 gtcttcgacc atccagcttg agtttgcgtc ctgaagttgc tctttatccc agtcagagta
    39301 cgggacttta tctctgatgc ggcgaacgta ttttttagcc tcgagttgat actggcctgc
    39361 cgaattaata aacgagaaat tcatgccgcc tttatccgct tctttggcct gcgccaccag
    39421 cgtttccagt tcagacactt tcttcattgc cgcttctgca tcaaacttat cttgcgatat
    39481 caggttatta atttgtttcg ctgaaatcat gactgccaga gagtagtagt ggaatgtttt
    39541 cccttctctt tcttcaatat ttttcagttc cgcaagctga cgcttatcat taatttcctg
    39601 aatcgccgca tgataagatt cggctacagg ttcaaaggct tccagatttt ttaagaatgt
    39661 ctggtgcagc gttttccctt tcgcaaacgc atcgtcttta taattttcct gggtgtaata
    39721 tttatccatt tcgttaatgg tattacccaa cgccacggca gcatcgatat aactcaccgc
    39781 cacgccatca attggttgca gcgcaggcgt taacgccaca gcacttttta cgcctttttc
    39841 acactcagcg aggttggatt cactaatgcc gtagatgcca taaacagtac gctcttcacc
    39901 ggtaggcccc tgtttaaaat ctttcagcca gtcagcataa cgcgccagac tgcgctgtac
    39961 cgggatctgt aacttgttgt agcacttgat atagacatcc attttttcgt cgaaaaccgc
    40021 ttgttcatcg accaccggtt gcgcaggtgt ttcaggttta gcttctgctt ttgcaacggg
    40081 agcttcagta ggtttcgctt caggagctgg tgcggcaggt tgactattgg caggcgggag
    40141 cgtttctgtt tcggcttttt tatcatcaca acccgtcaga ccgagggaca tgatggcgac
    40201 tataatagcg gaagataata aattcctttt catttaattg ccttataaat atatagataa
    40261 gagagtgaac ataataaatg aaaaaagaaa acgccactac acgcattttg tttataaata
    40321 aaataataaa attcacgaaa tgaataagtc ctgcaattta atatatttcg caggacttat
    40381 tttaattaat tgctcaaacg ctgcggatgg gtataaaccg ttgcccgacc cggtttacaa
    40441 aaacctacca gtgtcagatt acagcgctca gccacttcta cagcaagcgt ggtcgcggca
    40501 gacaccgcaa acaaaatctc tacgccgcac atcgccgact tttgcaccat ttcataactg
    40561 gcacgactgg aaaccagtac cgcaccttgc tgccagcttt ccccttcttg tgaccggcgg
    40621 cctaacagtt tgtccagcgc cacatggcga cccacgtctt catgcccgcc gaccagttcg
    40681 ccagatggca acatccaggc agcggcgtga gtacaaccag tcagttgccc cactggctga
    40741 aaatcgttga gatgacgtaa tgcaccatcc agtttgttga gatcaaacgt ctgggtgaac
    40801 ggtagcggct gcaccggttt tccgatgtca ttaagttgct ccacgccgca tacgccacag
    40861 cccgtacgtc ccgccagcgc ccggcggcgc tccttcaacc ccataaagcg gcggctggaa
    40921 agctcaattt gtacttcaag accattacag gaaggaacga catccatgcc gaagatatcg
    40981 cgcggacttt cgataatccc ctcggaaagc gaaaaaccga gcgcaaagta ctcaaggtct
    41041 ttgggcgacg ccatcatcac cacatgcgaa atgccgttgt agaccagcgc aacgggaact
    41101 tcttccgcga cctcatctaa ccgtgggtgt tgtaaatcat cacgccgcca taactcaatt
    41161 tgacgaacac ctgtgatatt tgtcacattt tcgatttctt ttcgctgtgt ttttttcact
    41221 ttctcttaac cagataagaa cacacacacc aacattatgg tattctgtta caaacccttc
    41281 ctggatggag ggaaattgag ccaattctgg acctttgcgg ccccttccgc aaagaaaaat
    41341 aactcccact ccctgcacac gcagcaagcg aatgtaaatg ggacgtgaca atgtcgaaac
    41401 aaggagcaat ccatgcaggt cagcagaagg cagttcttta agatctgcgc tggcggtatg
    41461 gcaggcacca cggcagcggc actgggtttt gcacccagcg tagcactcgc ggaaacccgg
    41521 cagtataaac tgctgcgcac ccgcgaaacc cgtaatacct gcacctattg ttccgtaggc
    41581 tgtgggctgt tgatgtacag cctcggtgac ggagcaaaaa acgccaaagc atctatcttc
    41641 catatcgaag gcgatccgga tcacccggtc aaccgcggtg cactttgtcc gaaaggcgct
    41701 ggcctggtgg atttcatcca ctccgaaagc cgtctgaagt ttccggaata ccgtgcgcca
    41761 ggttctgata aatggcagca aatcagttgg gaagaggcgt ttgatcgcat cgccaaactg
    41821 atgaaagaag accgcgatgc taactacatt gcgcaaaacg ccgaaggcgt gactgttaac
    41881 cgctggctct ccaccgggat gctgtgtgct tccgcgtcga gtaacgaaac cggctattta
    41941 acgcaaaaat tctcccgcgc gctgggtatg ctcgcggtcg acaaccaggc gcgtgtctga
    42001 cacggaccaa cggtagcaag tcttgctcca acatttggtc gcggtgcgat gaccaaccac
    42061 tgggtcgaca tcaagaacgc caacctcgtc gtggtgatgg gcggtaacgc cgctgaagct
    42121 cacccggtcg ggttccgctg ggcgatggaa gccaaaattc acaacggcgc gaagctgatt
    42181 gtgatcgatc ctcgctttac gcgtacggcg gcggtagctg actactatgc ccctattcgt
    42241 tccggtactg acattgcttt cctgtcaggc gtattgctgt acctgctgaa caatgaaaaa
    42301 ttcaaccgcg aatacaccga agcctatacc aacgccagcc tgatcgtgcg tgaggattac
    42361 ggctttgaag atggcctgtt caccggctac gacgcggaaa aacgcaagta cgataaatcc
    42421 tcctggactt atgaactgga cgaaaacggc ttcgccaaac gcgataccac gctgcaacat
    42481 ccgcgctgcg tgtggaactt gctgaaacag cacgtttccc gctacacgcc agatgtggtt
    42541 gaaaacatct gtggtacgcc aaaagacgcg ttcctgaaag tctgcgaata catcgcagaa
    42601 accagtgctc acgataaaac tgcctcgttc ctgtatgccc tcggctggac gcaacactcc
    42661 gttggtgcgc aaaacattcg tacgatggcg atgatccagc tgctgctcgg caacatgggg
    42721 atggcaggcg gcggcgttaa cgccctgcgc ggtcactcca atattcaggg gctgacggac
    42781 ctggggctgc tgtcgcagag cctgccaggt tacatgacgc tgccaagcga gaagcagacc
    42841 gatctgcaaa cctaccttac cgccaacacg ccaaaaccgc tgctggaagg ccaggtaaac
    42901 tactggggca actacccgaa attcttcgtc tccatgatga aggccttctt tggtgataaa
    42961 gcgacggcgg aaaatagctg gggctttgac tggttgccga agtgggataa aggctacgac
    43021 gtcctgcaat acttcgagat gatgaaagag ggcaaggtca atggctatat ctgccagggc
    43081 tttaaccctg ttgcctcgtt cccgaacaaa aacaaagtga tcggttgtct gtcgaaactg
    43141 aagttcctcg tcaccatcga cccgctgaac actgaaacct ctaacttctg gcagaaccac
    43201 ggtgagctga acgaagttga ctcgtcgaag atccagaccg aagtgttccg tctgccatcg
    43261 acctgcttcg cagaagagaa cggttcaatc gttaactcag gtcgctggtt gcagtggcac
    43321 tggaaaggtg cggacgcccc ggggattgcg ctgactgacg gtgagatcct ctccggtatc
    43381 ttcctgcgct tgcgcaagat gtatgccgaa cagggtggcg cgaacccgga tcaggtgctg
    43441 aacatgacct ggaactacgc tattccgcat gagccatctt cagaagaagt ggcgatggag
    43501 agcaacggta aggcgctggc cgatattacc gatccggcaa ccggtgcggt tatcgtcaag
    43561 aaaggccaac aacttagctc gtttgcccaa ctgcgcgatg acggtacaac ttcctgtggt
    43621 tgctggattt tcgccggtag ctggacgccg gaaggcaacc agatggcacg ccgtgataac
    43681 gccgatccgt ctggcctcgg taacacgctg ggctgggcat gggcatggcc gcttaaccgc
    43741 cgcattctgt ataaccgcgc ctccgcagat ccgcagggta acccgtggga tccgaagcgt
    43801 cagttgctga aatgggacgg cactaagtgg accggctggg atattccgga ctacagcgca
    43861 gcgcctccgg gcagcggcgt cgggccgttt atcatgcagc aggaaggcat gggacgtctg
    43921 tttgccctcg ataagatggc ggaaggtccg ttcccggaac actacgagcc gtttgaaacg
    43981 ccgctgggaa ctaacccgct gcatccaaac gttatctcga acccggctgc gcgaatcttt
    44041 aaagacgacg ccgaagcatt gggtaaagcc gataagttcc cgtatgtcgg aaccacctat
    44101 cgtctgaccg agcacttcca ctactggacc aaacacgcgc tgttgaacgc gattttgcag
    44161 ccagagcagt ttgtggaaat cggtgagtcg ctggcgaata aacttggcat tgcccagggc
    44221 gataccgtga aagtctcctc caaccgtggc tatatcaaag ccaaagcggt ggtgaccaaa
    44281 cgtattcgca cgctgaaagc gaacggcaaa gatatcgata ccatcggtat tccgattcac
    44341 tggggctatg aaggcgtagc taaaaaaggc tttattgcca atacgttaac gccattcgtt
    44401 ggtgatgcga acacgcagac gccggagttt aagtccttcc ttgtgaatgt ggaaaaggtg
    44461 taacggagac gacatatggc ttatcaatct caagatatca ttcgtcgttc cgcgactaac
    44521 ggtctgaccc ccgcgcctca agcgcgggac ttccaggaag aagtggcgaa actcatcgac
    44581 gtcaccacct gtatcggctg taaagcctgt caggtggcgt gttcagagtg gaacgacatt
    44641 cgcgataccg tcggcaataa cgttggggtg tacgacaacc ccaatgattt aagcgccaaa
    44701 tcgtggacgg taatgcgctt ctcggaagtg gagcagaacg acaaactgga atggctgatc
    44761 cgcaaagacg gctgtatgca ctgttccgat ccaggctgcc tgaaagcgtg cccggcggaa
    44821 ggtgcaatta ttcagtatgc caacggtatc gtcgacttcc agtccgagca gtgcattggc
    44881 tgcggttatt gcattgcggg ctgtccgttc gacattccgc gcctcaaccc ggaagacaac
    44941 cgcgtctaca aatgtacgct gtgcgttgac cgcgtggtgg ttgggcaaga accagcctgc
    45001 gtgaagacct gcccaacggg cgcgattcac ttcggtacga aagagtcgat gaaaacgctg
    45061 gcgagcgagc gcgttgctga gctgaaaacc cgcggttacg acaatgcggg tctgtacgat
    45121 ccggcaggcg tcggtggtac acacgtcatg tacgtgctgc accatgctga caagccaaat
    45181 ctgtatcatg gcttgccgga gaacccggaa atcagcgaaa ccgtgaaatt ctggaaaggc
    45241 atctggaaac cgctcgcggc tgttggcttt gcggctacct ttgcggccag tatcttccac
    45301 tacgtgggtg tcggtccgaa ccgtgcggat gaggaagaga ataatctgca cgaagagaaa
    45361 gacgaggagc gcaaatgaaa cgacgtgaca ccatcgtgcg ctacaccgcg ccggaacgta
    45421 tcaaccactg gatcaccgcc ttctgcttca tcctggcggc ggtgagcggg ctgggctttt
    45481 tgttcccgtc cttcaactgg ttgatgcaaa tcatgggcac accgcagctg gcgcgaattc
    45541 tgcacccgtt tgtcggcgtg gttatgtttg cctcgttcat catcatgttt ttccgttact
    45601 ggcatcacaa cctaatcaat cgggatgata tcttttgggc gaagaatatt cgtaagatcg
    45661 tcgtcaacga ggaagtaggt gacaccgggc gttataactt cggtcagaaa tgcgttttct
    45721 gggcggcgat tattttcctg gttctgctgc tggtgagcgg cgtgattatc tggcgtcctt
    45781 attttgcgcc tgctttctca atcccggtga tccgattcgc gttaatgctg cattcatttg
    45841 ccgcagtggc gttaattgtg gttatcatgg tgcatatcta cgccgccctt tgggtgaaag
    45901 gcacgattac cgccatggtg gaaggatggg ttaccagcgc ctgggcgaag aaacatcacc
    45961 cgcgctggta ccgtgaggtc cgcaagacaa cggaaaagaa agctgaatga gtattcgcat
    46021 aatcccgcaa gatgagctgg gttcaagcga gaaacgtacg gcggatatga ttccgccgtt
    46081 attgttccct cggctcaaga atttatacaa ccgccgcgcc gagcgtctgc gcgagctggc
    46141 agaaaataat cctctgggtg attacctgcg ctttgctgcg cttatcgccc acgcccagga
    46201 agtggtgctg tacgaccatc cgctggagat ggatctgact gcgcgcatta aagaagccag
    46261 cgcacaaggc aagcccccgc tggatattca cgttctgccg cgtgataagc actggcaaaa
    46321 gctgctgatg gcgctgattg ctgagctgaa acctgaaatg agcggcccgg cgctggcagt
    46381 gattgagaat ctggagaagg catcgactca ggagctggaa gatatggcca gcgcgctgtt
    46441 tgcctctgat ttctcgtccg tcagcagcga taaagcgccg tttatctggg ctgcactgtc
    46501 gctctactgg gcgcagatgg ccaatctgat ccccggcaaa gcccgcgctg aatacggcga
    46561 gcaacgtcaa tattgcccgg tttgtggttc tatgccggtg tccagcatgg tgcaaattgg
    46621 caccactcag gggctacgtt acctgcactg caacctgtgt gaaactgaat ggcacgtagt
    46681 gcgcgtaaaa tgcagcaact gcgagcagag cggcaaactg cattactggt cgctggatga
    46741 cgaacaggcc gcgattaaag ccgaaagctg cgatgactgc ggcacttacc tgaaaattct
    46801 ctatcaggaa aaagatccaa aaatcgaagc cgtagcagat gacctcgcct ctctggtact
    46861 ggacgcacga atggagcaag aaggctatgc ccgcagttcc atcaacccgt tcctgtttcc
    46921 gggtgaagga gagtaatctc tgacgttttc cgggtggcgc aggtcgcccg gattacagct
    46981 catagggtgt gacgacacca tctctcgtat tccgcgttac cacctcaagc tatgccgcca
    47041 gtgccggttt ggcctcaatt tacccaccac cacatcaata aggttatggg catgagactg
    47101 atgacaagcg taagattgcc tgatgcgctg cgcttatcag gcctaccaat aaactgcaat
    47161 acattgaatt tatacgactt tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
    47221 caacgcgcac gttgtcagca atctgattga catcattttt tcccattacc attcctcgcg
    47281 cagcttacgc tgatattcga caccatcttc ctcacaccct tgccagatgc caggaacact
    47341 ggttcttgct gtttgcgatt gatttatcag gtattgatct accgcttccc ttaataggtc
    47401 cgcacgagga agattacgct gcacctcgag atcatcaagt tgcttaatca cctcattcga
    47461 taaatcgagt aaaattctgc ccatatctat acctgccaga gagttcataa atatctcgcc
    47521 aatatcattt ttgaatctat ggagagaaaa gtacccttgc cgaatcttta aagaaagctc
    47581 atttacgcat cattttttat ttttgcgagg cgttttccag atatgatgta acagttgcag
    47641 ttattataag ttcaagtaaa acagccttgt attaatacca gaataagggc atctgttttt
    47701 tatattcaag aatgaaaaat ttttgtcatt ccttatgctc cttacactga tcatcaacac
    47761 caactacctc ccccttttgc ctgcctccct ttgctttacg caccgggaat tgctgacgtt
    47821 ccagatattg ctctaccgct tcgcgtaaaa ttgcagatcg tgaaacctta cgccagcccc
    47881 gaagcttgtt cagccgttta attgcctctt ctgataaatg aagaaaaacc gtattcatcg
    47941 ccattaccac caccttacct atattactca ttaatgtatg tgcgaaaatt acgccggaaa
    48001 atcatttaaa aaaagcggat ttttgcatgt tgttttgttt ttgcgaggcg ttttccagaa
    48061 ataataagag tgttgcagtt gtttcattta ttactcaaaa atggtttagt tatcgcccag
    48121 gatgaattat gggtgctatg aatatattat attcagagat atttatttgc ataatctgaa
    48181 tggtgtagcc gaaaaacgtc agcatcctaa cagcaccagg atttaaggtg aaattaatct
    48241 ttcatcacag accggtcagt actgccacca accggtcttt acttgctcat cgttataatt
    48301 ttgttggtaa cagttcggga aagttgtttt tctaaattaa caacagaata actttcttgt
    48361 ttaatgagat taatattggc aa
//
LOCUS       DBANUU010000020        47228 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000020 DBANUU010000000
VERSION     DBANUU010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.020"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..685)
                     /locus_tag="ACIE68_001104"
     CDS             complement(<1..685)
                     /locus_tag="ACIE68_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFX5907453.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGK"
     gene            complement(696..1697)
                     /gene="ltaE"
                     /locus_tag="ACIE68_001105"
     CDS             complement(696..1697)
                     /gene="ltaE"
                     /locus_tag="ACIE68_001105"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFX5907454.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1734..3452)
                     /gene="poxB"
                     /locus_tag="ACIE68_001106"
     CDS             complement(1734..3452)
                     /gene="poxB"
                     /locus_tag="ACIE68_001106"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFX5907455.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3585..4553)
                     /gene="hcr"
                     /locus_tag="ACIE68_001107"
     CDS             complement(3585..4553)
                     /gene="hcr"
                     /locus_tag="ACIE68_001107"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFX5907456.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4565..6217)
                     /gene="hcp"
                     /locus_tag="ACIE68_001108"
                     /gene_synonym="priS"
     CDS             complement(4565..6217)
                     /gene="hcp"
                     /locus_tag="ACIE68_001108"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFX5907457.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6361..7260)
                     /gene="lysO"
                     /locus_tag="ACIE68_001109"
     CDS             complement(6361..7260)
                     /gene="lysO"
                     /locus_tag="ACIE68_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFX5907458.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(7755..8450)
                     /gene="aqpZ"
                     /locus_tag="ACIE68_001110"
     CDS             complement(7755..8450)
                     /gene="aqpZ"
                     /locus_tag="ACIE68_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFX5907459.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGTTDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            8876..10534
                     /gene="ybjD"
                     /locus_tag="ACIE68_001111"
     CDS             8876..10534
                     /gene="ybjD"
                     /locus_tag="ACIE68_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFX5907460.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(10531..11487)
                     /gene="ybjX"
                     /locus_tag="ACIE68_001112"
     CDS             complement(10531..11487)
                     /gene="ybjX"
                     /locus_tag="ACIE68_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFX5907461.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            11638..12753
                     /gene="macA"
                     /locus_tag="ACIE68_001113"
     CDS             11638..12753
                     /gene="macA"
                     /locus_tag="ACIE68_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFX5907462.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQELDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            12750..14696
                     /gene="macB"
                     /locus_tag="ACIE68_001114"
     CDS             12750..14696
                     /gene="macB"
                     /locus_tag="ACIE68_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFX5907463.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(14769..14993)
                     /gene="cspD"
                     /locus_tag="ACIE68_001115"
     CDS             complement(14769..14993)
                     /gene="cspD"
                     /locus_tag="ACIE68_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFX5907464.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            15143..15217
                     /gene="yljB"
                     /locus_tag="ACIE68_001116"
     CDS             15143..15217
                     /gene="yljB"
                     /locus_tag="ACIE68_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFX5907465.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            15316..15636
                     /gene="clpS"
                     /locus_tag="ACIE68_001117"
     CDS             15316..15636
                     /gene="clpS"
                     /locus_tag="ACIE68_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFX5907466.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            15667..17943
                     /gene="clpA"
                     /locus_tag="ACIE68_001118"
     CDS             15667..17943
                     /gene="clpA"
                     /locus_tag="ACIE68_001118"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFX5907467.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(18287..18374)
                     /locus_tag="ACIE68_001119"
     tRNA            complement(18287..18374)
                     /locus_tag="ACIE68_001119"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18338..18340),aa:Ser,seq:gga)
     gene            complement(18628..18846)
                     /gene="infA"
                     /locus_tag="ACIE68_001120"
     CDS             complement(18628..18846)
                     /gene="infA"
                     /locus_tag="ACIE68_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFX5907468.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(19131..19835)
                     /gene="aat"
                     /locus_tag="ACIE68_001121"
     CDS             complement(19131..19835)
                     /gene="aat"
                     /locus_tag="ACIE68_001121"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFX5907469.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(19877..21598)
                     /gene="cydC"
                     /locus_tag="ACIE68_001122"
     CDS             complement(19877..21598)
                     /gene="cydC"
                     /locus_tag="ACIE68_001122"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFX5907470.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(21599..23365)
                     /gene="cydD"
                     /locus_tag="ACIE68_001123"
     CDS             complement(21599..23365)
                     /gene="cydD"
                     /locus_tag="ACIE68_001123"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFX5907471.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(23488..24453)
                     /gene="trxB"
                     /locus_tag="ACIE68_001124"
     CDS             complement(23488..24453)
                     /gene="trxB"
                     /locus_tag="ACIE68_001124"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFX5907472.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            24997..25491
                     /gene="lrp"
                     /locus_tag="ACIE68_001125"
     CDS             24997..25491
                     /gene="lrp"
                     /locus_tag="ACIE68_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFX5907473.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            25626..29732
                     /gene="ftsK"
                     /locus_tag="ACIE68_001126"
     CDS             25626..29732
                     /gene="ftsK"
                     /locus_tag="ACIE68_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFX5907474.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQLKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            29887..30498
                     /gene="lolA"
                     /locus_tag="ACIE68_001127"
     CDS             29887..30498
                     /gene="lolA"
                     /locus_tag="ACIE68_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFX5907475.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            30509..31852
                     /gene="rarA"
                     /locus_tag="ACIE68_001128"
                     /gene_synonym="mgsA"
     CDS             30509..31852
                     /gene="rarA"
                     /locus_tag="ACIE68_001128"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFX5907476.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            31943..33235
                     /gene="serS"
                     /locus_tag="ACIE68_001129"
     CDS             31943..33235
                     /gene="serS"
                     /locus_tag="ACIE68_001129"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFX5907477.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            33474..35918
                     /gene="dmsA"
                     /locus_tag="ACIE68_001130"
     CDS             33474..35918
                     /gene="dmsA"
                     /locus_tag="ACIE68_001130"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFX5907478.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPQWASQITGIPAEKIIQLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNTGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            35929..36546
                     /gene="dmsB"
                     /locus_tag="ACIE68_001131"
     CDS             35929..36546
                     /gene="dmsB"
                     /locus_tag="ACIE68_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFX5907479.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            36548..37411
                     /gene="dmsC"
                     /locus_tag="ACIE68_001132"
     CDS             36548..37411
                     /gene="dmsC"
                     /locus_tag="ACIE68_001132"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFX5907480.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(37447..38073)
                     /gene="ycaC"
                     /locus_tag="ACIE68_001133"
     CDS             complement(37447..38073)
                     /gene="ycaC"
                     /locus_tag="ACIE68_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFX5907481.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNIVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDTPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            38387..39535
                     /gene="ycaD"
                     /locus_tag="ACIE68_001134"
     CDS             38387..39535
                     /gene="ycaD"
                     /locus_tag="ACIE68_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5907482.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            39745..41175
                     /gene="ycaM"
                     /locus_tag="ACIE68_001135"
     CDS             39745..41175
                     /gene="ycaM"
                     /locus_tag="ACIE68_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFX5907483.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGRGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(41176..42084)
                     /gene="ycaN"
                     /locus_tag="ACIE68_001136"
     CDS             complement(41176..42084)
                     /gene="ycaN"
                     /locus_tag="ACIE68_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFX5907484.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            42184..42774
                     /gene="ycaK"
                     /locus_tag="ACIE68_001137"
     CDS             42184..42774
                     /gene="ycaK"
                     /locus_tag="ACIE68_001137"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFX5907485.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(42856..43596)
                     /gene="pflA"
                     /locus_tag="ACIE68_001138"
     CDS             complement(42856..43596)
                     /gene="pflA"
                     /locus_tag="ACIE68_001138"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFX5907486.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(43788..46070)
                     /gene="pflB"
                     /locus_tag="ACIE68_001139"
     CDS             complement(43788..46070)
                     /gene="pflB"
                     /locus_tag="ACIE68_001139"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFX5907487.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQTDLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(46125..46982)
                     /gene="focA"
                     /locus_tag="ACIE68_001140"
     CDS             complement(46125..46982)
                     /gene="focA"
                     /locus_tag="ACIE68_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFX5907488.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT        11690 a        11738 c        12482 g        11318 t
ORIGIN      
        1 gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc acctctggtc
       61 cggcggcttc gaagatgcgg tgttcgctgg ccggatgatc taacaacgcc accagatagt
      121 gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt ggcggcgtta
      181 acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa cctgcgccaa
      241 cgataattcc ggcccgaagt tcggtcacag gtacattcgc ttcacgaaga atgtccgccg
      301 tagcctgacg agcacgcaga tgatccgact gctcatgtgg cggggcctgc aacgaactga
      361 gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg agagccacct
      421 ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga aaatagacgg
      481 tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg actttatggc
      541 aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc gccgccagga
      601 tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag ccactggcac
      661 cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa tgcgcgccag
      721 tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac caggcggaca
      781 atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa cgcggcagca
      841 ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac atccgcgcct
      901 gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg caaccgtgcg
      961 acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg acgcatcccg
     1021 ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt accgacgagt
     1081 aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga atcacaatat
     1141 tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat gcgcgcaccg
     1201 tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt caggtattca
     1261 cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt gcgggcgaaa
     1321 tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt gccgtcggca
     1381 gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc ggcttcaaac
     1441 agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca gtgactgagc
     1501 agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc tttaccggaa
     1561 agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta aacgtcgtcc
     1621 ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt aacggtatca
     1681 ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc tttttacctt
     1741 agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat aattgcgcgc
     1801 aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg aatggctaac
     1861 tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa ggcgcgttgc
     1921 agggcttcat caacttcaga cgctttttct acacggatac ccgtaatgcc gcacgcttcg
     1981 gcaatgcggg caaagtttgt gtcgtgtagt tcggtgccgt cagtcaaata gccaccagct
     2041 ttcatctcca tcgccacaaa gcccagcacg ctgttgttaa agacgacaat tttcactggc
     2101 agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc gccatcgccg
     2161 cacatggcga ccacctgacg ttctggctct gtcgcctgcg cacccagcgc ctgcggcatg
     2221 gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc gttcattttt
     2281 agataacgtg ccgcccacac cgttggcgta ccaacgtcac aggtgaaaat agcgtcatcg
     2341 gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt ctcgctcggt
     2401 ttagctaaat cgtccagccc tttgcgggcg tcgcggtaat cttccagcgc tttatccaga
     2461 aacttgcgat cggctttttc ttccaccaat ggaagcaatg cacgcagagt cgacttgata
     2521 tcgccgacca gtgccatatc caccttgctg tgagcgccga tgctggctgg gttgatatca
     2581 atctgaatga ttttggcatc ggtcgggtag aaggcgcggt agggaaattg cgtgccgagt
     2641 agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaagcc gattaacccg
     2701 gtcattccaa catcatacgg attatcgtat tcgacatgtt ctttaccgcg cagggcatga
     2761 acaataggcg ctttaatttt cccggcaaac tcaactaact ctttatgcgc ccccgcgcag
     2821 ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc cagtttgcgt
     2881 aactcttctt cttccggcgt cacgactggt tgtggcgcat gataccagtg cgtggttgcc
     2941 ccttctggcg caggttttaa cgccacgtcg cctggtaaca cgacaaccga aacgccacgg
     3001 ttaagcaccg ctttgcgcat ggcaatcgcc agtacttgtg ggatctgctc cgggctggaa
     3061 accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt ttcctggaaa
     3121 tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac cggaacgtga
     3181 ttgcggtggc aatcgaacag gccgttgatt aagtgcaggt tgccggggcc gcacgatccg
     3241 gcgcagaccg ccagttctcc gctaagttgt gcttcagcgc cagcggcaaa ggccgccact
     3301 tcttcgtggc gggtggacat ccactcgatg gtgcccatgc gattaagact gtcactaaga
     3361 ccgttcagag agtcgccagt gactccccag atgcgtttca cccctgccga ttcgagtgtt
     3421 ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa tgtgataacg
     3481 gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg aggcactaac
     3541 ggttaaatag cccgatgaaa ggaatatcat cgggtataag gcgattatgc gagaaccaaa
     3601 tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc ggcgtcggtc
     3661 agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt acagcagccg
     3721 caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag cgcctccagt
     3781 agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt gaatttcaga
     3841 ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa acgcgtcacg
     3901 ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc gcaggtcatc
     3961 acggtacgtg aagctaagtc aggtacacct gccagcagtt cgcgagtgag acgaccagcg
     4021 ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt acgccactca
     4081 tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac atcggcctgt
     4141 ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc gcagcctgcc
     4201 gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc catcgcgtcc
     4261 gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga gccgacaccg
     4321 tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt ggaggaaatg
     4381 gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata ttgcccggcg
     4441 cgatatgggt agtaatcgtg gcaaatcagg gaaatcgtcc acacatccgg cgtttcttgc
     4501 gtaatgtgat gaacttgcat ccgccacggg cattgattcg ttggcatcgt catcgacaaa
     4561 ctccttacgc gctcaacagt tgcttcatgt cttcttcaac agtggtgata gaacgcaggc
     4621 caaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaaccaggt gcagtcgggc
     4681 cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgacg atcgctttct
     4741 gttcaaacca ggagagcacc agcgacagcg gcagatcgtt gacaccgcag cccagtttct
     4801 ctgccagagt gacagccaga ataatcgctg agtaagcatc gttacattga cctgcatcta
     4861 ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga tatttaccac
     4921 aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg gtgaagtagt
     4981 ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc agtttttcac
     5041 ggctcaccag atcaatcagc gtatccgctg cgccaagcag cgtctggcgg ccaaagccca
     5101 cggtgatcag gtgcggaatt tcgctgtacg ggaagcccgc catttgttgc gcctgggcga
     5161 tgaccgcaga aaaatcttcg ccatccagat gacgtacacc cggccagcca acgatgctgc
     5221 gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag ttcgaggtca
     5281 tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag ccgctgccgt
     5341 agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca ggcagcattt
     5401 cgccgtgggt gtaaacatta acgcccgtgc cttcggtctg ttccagcagg ttgtagagat
     5461 ctttgagatc gtgaccggaa atcaggatgc atttacccgc cgtcgctttg acgttgacct
     5521 gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg ctcatcactt
     5581 tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata tcggcaggcc
     5641 aggtccccag ccacgccatg attttatgat actgggcata aatatcgttg tcgtattgac
     5701 cgagaacatg cgcgtgttcc atataggccg ctgcaccttt caggccatac aggcacagca
     5761 gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg gtaaattctg
     5821 ctgcctgacg ttgcagctcg ccgagatcgt cgctcaccag ttgcaggtca gccatcgggt
     5881 tatcgacgcg cgcgttggca tctacagcca ggcattgcgc tttcagcgcc tcgcgcaggg
     5941 caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg ttggtcaggg
     6001 ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg ccgtattcac
     6061 gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa tcctgaaggt
     6121 cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg tttcctgccg
     6181 gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt aaagttatat
     6241 ttaatataca tgtttaaggt taagacgctt aacgcgggga taaaagggat ttttcatgca
     6301 actttaaggg agattgattt agcgcaattt tggcggcagg gatctaccgc cagagaggta
     6361 ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa aaccgtgaac
     6421 aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca gggtgaaatc
     6481 cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa tcagcccagg
     6541 gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg cgctcccgat
     6601 taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc cgaaaccgga
     6661 ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa ttaaaccacc
     6721 aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat taaggacaat
     6781 ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa gtagcaaaat
     6841 taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac tcagaccaat
     6901 ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca tcgcaatacg
     6961 cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct ccagccacat
     7021 cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat aatgcagaat
     7081 cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca taaaaaagag
     7141 aataaggtaa accatccagc ttaatagctg attaataact tttaacgcag cttgttggcg
     7201 aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc cagaaaacat
     7261 gaaaacccag tccttgcaaa gatgaagtcg aaatgcgcga tgacacacta ctgaaagcgg
     7321 aaggacgagt aaagttgcaa ttaaaaggaa atgttatgca taaggagcag tagagtattc
     7381 gttttcattt aaagatattc ttgcgcttta attacaaact gcaccgatgt tggtggcgtc
     7441 aaaatcgccg aggcgttccc tgaaggccgg ggcagcccac atggatgtgg gctgagggcg
     7501 cgttttacag ggatgttacc tcgcgcccga cccggtagcc gtaagggata agtcgagggc
     7561 accgcgcagc ggcgattttg ttcgccagag cccgggggtg cagggggcgg cggcgattgg
     7621 ccgccccctg cgcgctcttt gcgccagtgg caatatgtgg cttagctcat gaaaggagcg
     7681 caacaagatg atgaatcaac atataacaac atcttaaaaa aaggcctgac attacgccag
     7741 gccttctgcg ttaattaatc acgcttttcc agcagggtcc ggtaaatcag accaccgata
     7801 atgccgccga caattggcac cacccagaag aaccacagtt gttctaatgc ccagccgccc
     7861 tggaagatag caaccgcggt gctgcgcgcc gggttaacag aagtgttagt caccggaata
     7921 ctaattaagt gaatcagggt taaggccaga ccaatagcga tcggcgcaaa acctgccggc
     7981 gcgaatttgt cggttgtgcc gtggatcacc aacaggaaac ctgcactcaa taccagttca
     8041 actaccagcg cggaaagcat ggaataaccg cctggtgaat gctcgccata accgttagaa
     8101 gcaaaaccgc tggctgccgc gtcaaaaccc gttttaccac tggcaattaa atacagcagc
     8161 gccgctgcaa caataccgcc gacaacctgg gcaattacgt agccaacgac ttcttttgcc
     8221 ggaaaacgtc cgccagccca taaaccaata gtgaccgccg ggttaaaatg accaccagaa
     8281 atatgaccaa cagcaaaggc catcgtcaga acggtcagac cgaacgccaa cgccacgccg
     8341 gcaaaaccaa tgcctaattc cgggaagcct gcggccagta cagcactacc acagccacca
     8401 aaaacaagcc agaaagtacc aaaacattca gctgctaatt ttctgaacat atccacctca
     8461 attaaaaatt gaccctgtga aaaatatggt cgttttatag ggtcgtcgta aaaaagtgac
     8521 gacggaaata atgcgcggct attttaaaaa cgaaggcgag tcattcacca gataaataaa
     8581 tccagtaaat ttgatttagg gcaacagcgg gttgccccat atagtcattt gtctgattga
     8641 cagtgtagtg caagcaaaag atttaatcct ttaggcgtaa taaaaaataa tttatcatgc
     8701 taattatttg attttgttgt ttttgcagac ttatcagcaa gagggagtat aacgcgatta
     8761 ttcgctcatt tttcagacat ttgccatgct taaatgtgat gtcatcacgt attagcaagg
     8821 cctttcccgt tatactgcca gcgtaaagga taagtcacat atttctggag gggatatgat
     8881 tcttgagcgc gttgaaattg tgggttttcg cggtatcaac cgtttgtcgt tgatgctcga
     8941 acaaaacaac gtcctgattg gggagaacgc gtggggtaaa tccagcttgc tggacgcctt
     9001 aaccctgctg ctatcgccag aatcagatct ctaccatttt gagcgcgacg atttctggtt
     9061 cccgccggga gatatcaacg ggcgagaaca tcatctgcat attattttga ccttccgcga
     9121 atcgctgcca ggccgacatc gggttcgccg ttatcggccg ctggaagcgt gctggacgcc
     9181 atgcaccgat ggctatcacc gtatttttta tcgtctggaa ggggagagtg cggaagacgg
     9241 cagcgtgatg acactgcgca gttttctcga taaagacgga catccgattg atgtcgagga
     9301 tattaacgat caggcacgcc atctggtgcg tttaatgccg gtgctgcgct tgcgtgatgc
     9361 ccgttttatg cgccgtattc gtaacggcac ggtgccaaat gttcccaatg tggaagtcac
     9421 cgcgcgccag ctcgatttcc tcgcccgtga gttgtcctca catccgcaaa atctctctga
     9481 tggacagatt cgtcagggac tttccgcaat ggtgcagttg cttgagcatt atttctctga
     9541 gcagggggcc ggacaggcgc gatatcgttt aatgcggcgg cgggccagca atgagcaacg
     9601 aagctggcgc tatctggaca tcatcaaccg gatgattgac cgacctggag ggcgctcgta
     9661 tcgggttatt ttgctcggcc tgtttgctac tttattgcag gcaaaaggca cattgagact
     9721 ggataaagac gcgcgtccat tgttgctgat cgaagatcca gaaacccgtt tacaccccat
     9781 tatgctttca gttgcctggc atctgttgaa tcttctgcca ttgcagcgca ttgccaccac
     9841 caactcgggt gagttgcttt cgttaacgcc ggtagagcat gtttgccgac tggtacgtga
     9901 gtcctcgcgc gttgccgcct ggcgtctggg gccgagtggc ttgagtaccg aagatagccg
     9961 acgcatatcc tttcacattc gttttaaccg tccgtcatcg ctgtttgctc gctgctggtt
    10021 gctggtggaa ggggaaacgg aaacctgggt tatcaatgaa ctggcgcgcc agtgtggaca
    10081 ccatttcgat gccgaaggga tcaaggtcat tgagtttgcc cagtccgggc taaagccact
    10141 ggttaaattt gcccgccgaa tggggattga atggcatgta ctggtcgatg gcgatgaagc
    10201 agggaagaaa tatgccgcta cggtacgcag cctgttgaat aacgatcggg aagccgaacg
    10261 agaacattta acggcgttac cggcgctgga tatggaacat tttatgtatc gccagggatt
    10321 ttccgatgtg ttccaccgcg tggcgcaaat cccggaaaat gtaccgatga atctgcgcaa
    10381 aattatctcg aaagcgatcc atcgctcttc caaacccgat cttgccattg aagtggcaat
    10441 ggaggccgga cgtcgtggtg tggactccgt accgacgctg ctgaaaaaaa tgttctcacg
    10501 cgtgctgtgg ctggcgcgcg gtcgcgcgga ttaaccgcga aacatcgtgg ccatttgtgg
    10561 ctgaatagcg tcgagcatct catagcgccg acggtattca gcccgttttt tactggcgat
    10621 ttcggcaatc tcttttcgtg ctatctgtgc tggaaggcgg taatggcgtt cagcatcaca
    10681 tacgccgcca accgattccc agaaagcgtt gtaatcggca tggatcttgc cttctttatc
    10741 acgataacgc aggctgcggt aaatatgcgt ttcattgctg acggcaataa tctgctctac
    10801 ctgcaaacgt tgggcaaaca gacaggccgc ttccatcacg aggcgtttgg gaaatagccc
    10861 gtggcaggct ttcgtcgcat tctggatttc ctgatgtgga atttcccatt ttgcgccttg
    10921 cagtccgcca ataaacatcg ttcttttccc ctgatattca cacagggtaa acgtgatctc
    10981 tgccagagga ataccttcgc tgttgcgaaa caggattgtg ctgtcacctt ctttatccat
    11041 tgagatcatc atggtcagct caagcgtgaa ctgctcgccg tttttgcctt ccagtttagc
    11101 cagttgcagc ccgggggtat tcaaatataa gctgaattct tccgccgaca tacatccgcg
    11161 gagtaacgca taatggtaac gtaacgcctc cagcaattgc ttacggctaa gattcgccgc
    11221 aaggtaaggg cgatgcagac gcacaggcag tcgcggttgg cgcgttaaca acacattgag
    11281 attaggccag tgggaaagtt cgttcatcca ctcaacgctt aaacgcggca taatcaacga
    11341 gcgcagcaaa aatttctggc gaaaactacg gcgatgccag aatttacccg gccgacactg
    11401 tccacgtgcc agactaagaa aaagtgacag gctgctgaga gattcagatg gcgtaaaggt
    11461 ccgttcagtt agctgcgaca tattcatgaa atcaatggtt atacatgacg tcgatttcac
    11521 cattgcgtat cttaaccaaa catcaatagt gtgattacta acgtaaattt tagggttttg
    11581 ttgatatttc gttgaagtta atgacccgga ttggcatatg gagtattcag aatatttatg
    11641 aaaaagcgga aaaccgtgaa gaagcgttac gttattgcgc tggtgatagt catcgccgga
    11701 ctgattacgt tatggagaat tcttaacgca cccgtgccga cttatcagac actgattgtg
    11761 cgccccggtg atttacagca aagcgtgctg gcgaccggaa agctggacgc gctgcgtaag
    11821 gttgacgtgg gcgcgcaggt cagcggtcag ttgaaaactc tgtcggtggc gattggcgat
    11881 aaagtaaaaa aagaccagct tttaggggtt attgatcctg aacaggctga aaaccagatt
    11941 aaggaggtcg aagcaacgct gatggagcta cgtgcgcagc ggcagcaggc ggaagcggag
    12001 ctgaaactgg cgcgggtgac gtattcccgt cagcaacgtc tggcacaaac gcaggcggtt
    12061 tcacagcagg agctcgacac cgccgcgacg gagatggctg tgaaacaggc gcaaattggc
    12121 accattgacg cgcaaatcaa gcgcaatcag gcctctctcg atacggctaa aaccaatctc
    12181 gattacactc gcatcgttgc cccgatggcc ggggaagtca cgcaaatcac cactctgcaa
    12241 ggccagacgg tgattgccgc acaacaagca ccgaacattc tgacgctggc agatatgagc
    12301 accatgctgg taaaagcgca ggtttctgaa gcggatgtaa tccacctgaa gccggggcaa
    12361 aaagcctggt ttacggtact tggcgatcca ctgacgcgct acgaggggca aatcaaggat
    12421 gtactaccga cgccggaaaa ggttaacgac gctattttct attacgcccg ttttgaagtc
    12481 cccaacccca atggtttgct gcggctggat atgactgcgc aagtgcatat tcagctcacc
    12541 gatgtgaaaa atgtgctgac gatccctctg tcggcgttag gcgatccggt tggcgataat
    12601 cgttataaag tcaaattgtt gcgtaatggt gaaacacgcg agcgtgaagt gacgattggc
    12661 gcacgtaacg ataccgatgt tgagattgtc aaagggcttg aagcgggcga tgaagtggtg
    12721 attggtgagg ccaaaccagg agctgcacaa tgacgccttt gctcgaatta aaggatattc
    12781 gtcgcagcta tcctgccggt gatgagcagg ttgaggtgct gaagggcatc accctcgata
    12841 tttatgccgg tgagatggta gcgattgttg gcgcttcagg ttccggtaaa tcgaccctga
    12901 tgaatattct cggctgtctg gataaggcca ccagcggcac ctatcgcgtc gccggtcagg
    12961 atgttgccac actggacgcc gatgcgctgg cgcaactgcg ccgcgagcat ttcggcttta
    13021 ttttccagcg ttaccattta ctttcgcatt taaccgccga gcagaacgtt gaagtacccg
    13081 ccgtctatgc tggtcttgag cggaaacagc gactgcttcg tgcccaggag ttgctgcaac
    13141 ggctggggct ggaagaccgt acagagtatt atccggcaca gctttcgggt ggtcagcaac
    13201 agcgcgtcag catcgcgcgg gcattgatga acggtggtca ggtaattctt gccgatgaac
    13261 caaccggcgc actggacagc cattctggcg aagaggtgat ggcgatcctg catcagctgc
    13321 gcgatcgtgg gcatacggtg attatcgtca cccacgatcc gcaggtcgct gctcaggccg
    13381 agcgggtgat cgaaattcgc gacggcgaaa ttgtgcgcaa tcctcccgcc attgaaaaag
    13441 tgaatgttgc tggcgggacg gagcctgttg tcaacacggt gtctggctgg cggcagtttg
    13501 tcagcggttt taacgaggcg ctgacgatgg catggcgggc gctggcagcg aataaaatgc
    13561 gtactttact gaccatgctg gggattatta tcggtattgc gtcggtggtt tccattgtcg
    13621 tggtgggtga cgccgccaaa caaatggtgc tggcggatat tcgttctatt ggtacgaata
    13681 ctattgatgt ctatcccggg aaagattttg gcgatgacga tccgcaatat cagcaggcgc
    13741 tgaagtacga cgacttaatc gccatccaaa aacaaccgtg ggtcgcctca gccacacctg
    13801 ccgtctcgca aaacctgcgc ctgcgttata acaatgttga tgttgctgcc agtgccaatg
    13861 gcgtgagcgg cgattatttt aatgtctatg gcatgacctt cagtgaagga aacaccttta
    13921 atcaggagca gctgaacggt cgtgcgcagg tcgtggttct cgacagtaat actcgccgcc
    13981 agcttttccc ccataaagca gatgtggttg gcgaggtgat tctggtcggc aatatgcccg
    14041 ccagagtcat tggtgtggcg gaagaaaaac agtcgatgtt tggtagcagt aaagtgctgc
    14101 gtgtctggct accttacagc acgatgtccg ggcgagttat gggccagtcg tggctgaact
    14161 ccattactgt cagggtaaaa gaaggatttg acagcgccga ggcggaacag caactcacgc
    14221 gtttactttc actgcgccac ggaaagaagg atttctttac ctggaacatg gacggcgtct
    14281 tgaaaactgt tgaaaagacc acacgtactt tacaactgtt tctgacgctg gtggcggtga
    14341 tttcgctggt ggtgggcggt attggtgtaa tgaatattat gctggtgtca gtgaccgagc
    14401 ggacgcggga aattggcatt cgcatggctg taggtgcgcg agcaagcgat gttttgcaac
    14461 agttcctgat cgaagccgta ctggtttgcc tggtcggtgg cgcgttggga ataacactgt
    14521 cactgttaat tgctttcacc ttgcagcttt tcttacccgg ctgggagatt ggtttttcac
    14581 cgttggcact gctgctggcg tttctctgct cgacggtcac cgggatttta tttggatggt
    14641 tacccgcacg aaatgcggca cgactggatc cagtagatgc tctggcacga gagtaatttt
    14701 tgagataaaa atgccagccg atcgggctgg cattttgcct ttaggatgta cacaatgaga
    14761 cagaagagct atgcgactgc cgcttctact tcgacgggca caataacact ggcgtgattg
    14821 ccttttggcc cctggtggac atcaaactga acggattgtc cagcttttag cgttctgtaa
    14881 ccatccatct gaatggtgga ataatgagcg aaaatatctt cgccgccgcc ttcagggcag
    14941 atgaaaccaa accctttggc attgttgaac cacttaacag tacccttttc catgcttcga
    15001 catccttcgc aaatcttata caagtaagat ggaataaacc ggggtcagag agggggctgt
    15061 tcaaaacctc gccaactcta gaaatacaat ttagagaatt agggcgagcc gtcaagcgtt
    15121 tgacaggggc cagggggcag gtatgaatca aaaatttgaa gcagttaacg ctattgacag
    15181 gaatgtgaca gatgtcgctg atgccaacga tagatgatag ttatctatca tgtggagtag
    15241 attggtcagg caaataagct cttgtcagcg gcagggcgtt ctgccgataa ccgtaaccga
    15301 agatgataac tgacaatggg taaaacgaac gactggctgg actttgatca actggcggaa
    15361 gaaaaagttc gcgacgcgct aaaaccgcca tctatgtata aagtgatatt agtcaatgat
    15421 gattacactc cgatggagtt tgttattgac gtgttacaaa aattcttttc ttatgatgta
    15481 gaacgtgcaa cgcaattgat gctcgctgtt cactaccagg ggaaggccat ttgcggagtc
    15541 tttaccgccg aggttgcaga aaccaaagtg gcgatggtga acaagtacgc gagggagaat
    15601 gagcatccat tgctgtgtac gctagaaaaa gcctgaatgc aggcataaaa attgggggag
    15661 gtgcctatgc tcaatcaaga actggaactc agtttaaata tggctttcgc cagagcgcgc
    15721 gagcaccgtc atgagtttat gaccgtcgag cacttgttac tggcgctgct cagtaaccca
    15781 tctgcccggg aggcgctgga agcgtgttct gtggatttgg ttgcgctccg tcaggaactg
    15841 gaagccttta ttgaacaaac cacacccgtt ctgcctgcca gtgaagagga gcgcgacaca
    15901 cagccgacgc tgagttttca gcgtgtactg caacgtgcgg tcttccatgt ccagtcctcc
    15961 ggtcgcaatg aggtaaccgg tgcaaacgtt ctggtcgcta tctttagcga acaggagtcg
    16021 caggcggcat atctgttgcg taaacatgaa gtcagccgtc tcgatgtggt gaactttatc
    16081 tctcatggca cgcgtaaaga cgagccgaca cagtcttctg atcctggcag ccagccaaac
    16141 agcgaagaac aagctggtgg ggaggaacgt atggagaatt tcacgacgaa cctgaatcag
    16201 cttgcgcgcg tgggcggaat cgacccactg attggtcgtg agaaggagct ggagcgtgct
    16261 attcaggttc tctgccgtcg ccgtaaaaac aacccgctgc tggtggggga atctggtgtc
    16321 ggtaaaaccg cgattgcgga aggtcttgcc tggcgaattg ttcagggcga tgtgccggaa
    16381 gtgatggctg actgtacgat ttactctctc gatatcggtt ctctgttagc gggcactaaa
    16441 tatcgcggcg actttgaaaa acgttttaaa gcgttgctca agcagctgga gcaggacact
    16501 aacagcatcc tgtttattga tgagatccac accattatcg gtgcgggtgc agcgtctggt
    16561 ggccaggtcg atgcggctaa cctgatcaaa ccgttgctct ccagcggtaa aattcgcgta
    16621 attggttcga caacctatca ggagttcagc aacattttcg agaaagaccg tgctctggcg
    16681 cgtcgcttcc agaaaattga tattactgaa ccgtcgatcg aagaaactgt tcaaatcatc
    16741 aatggcctga aaccgaagta tgaagcgcac cacgacgtgc gttataccgc aaaagcggtg
    16801 cgtgcagcgg tagagctggc ggtgaaatac attaacgatc gtcatctgcc ggataaagcc
    16861 attgacgtta tcgacgaagc gggcgctcgc gcacgcctga tgccggtaag caaacgcaag
    16921 aaaaccgtta atgtggcgga tattgagtcc gtggtggccc gtattgcgcg cattccagag
    16981 aagagtgttt ctcagagtga ccgcgatacc ctgaaaaacc tcggcgatcg cctgaaaatg
    17041 ctggtcttcg gtcaggataa agccattgag gcgctgactg aagccattaa gatggcgcgt
    17101 gcaggtttag gtcacgaaca taaaccggtt ggttcgttcc tgtttgccgg ccctaccggg
    17161 gtcgggaaaa cagaggtgac ggtacagctt tcgaaagcgt tgggcattga gctgctgcgc
    17221 tttgatatgt ccgagtatat ggaacgccat accgtcagcc gtctgattgg tgcgcctccg
    17281 ggatacgttg gttttgatca gggcggtttg ctgactgatg cggtcatcaa gcatccacat
    17341 gcggtgctgt tgctggacga aatcgagaaa gcgcatccgg acgtgttcaa tattctgttg
    17401 caggtgatgg acaacggtac gctgaccgat aacaacggac gcaaagcgga cttccgtaac
    17461 gtggtgctgg tgatgaccac caacgccggg gtacgtgaaa ctgagcgtaa atccattggt
    17521 cttatccacc aggataacag caccgatgcg atggaggaga tcaagaagat ctttacaccg
    17581 gaattccgta accgtctcga caacattatc tggtttgatc atctgtcaac cgacgtgatc
    17641 catcaggtgg tggataaatt catcgtcgag ttgcaggttc agctggatca gaaaggtgtt
    17701 tctctggaag tgagccagga agcgcgtaac tggctggccg agaaaggtta cgaccgggca
    17761 atgggcgcac gtccgatggc gcgtgtcatc caggacaacc tgaaaaaacc gctcgccaac
    17821 gaactgctgt ttggttcgct ggtggacggc ggtcaggtca ccgtcgcgct ggataaagag
    17881 aaaaatgagc tgacttacgg attccagagt gcacaaaagc acaaggcgga agcagcgcat
    17941 taatctgatt gttaggtagg ttggtcaagt ccgtaatctc gaaagaggtt acggactttt
    18001 tgtttatggg gtggaggagg ttcagaccct ttttttaatg atgatggtaa gttgttgata
    18061 attagtgctg cgggaaggta aggataaaaa agggtgctgc aggagaatgg gatggttttg
    18121 ctttattaac aacgggctaa acgtgtagta tttgagttca ctgccgtaca ggcagcttag
    18181 aaatcgacgg ggtgcggtaa aacctttgcg aacgcgttca ctgccgtaca gacagataaa
    18241 atgcgaaaaa aaagctcgca ctttcgtacg agctcttctt taaatatggc ggtgaggggg
    18301 ggattcgaac ccccgatacg ttgccgtata cacactttcc aggcgtgctc cttcagccac
    18361 tcggacacct caccaaattg ttttgctacc aaacctcatg ggtggcaacg gggcgctact
    18421 atagggagtt ggagtaaaac ggtcaagaag aattttaatg ataattattg tttgctcata
    18481 ctgtaaacaa gttgtgcagt atatctacat cgagacaagt tacggactta tacttccaaa
    18541 gtacttcata catatcacaa aataaaaagg ccggttaaac cgacctttta ctcgttcttt
    18601 ctcttcgccc atcaggcggt aaaacaatca gcgactacgg aagacaatgc ggcctttgct
    18661 caggtcgtac ggggtcagtt caacagtcac tttgtcgccc gtcaggatgc ggatgtagtt
    18721 tttgcgcatt ttaccggaga tgtgtgcagt aaccacgtga ccgttttcta actctacgcg
    18781 gaacatggta ttaggcaacg tttcaagaac ggtaccttgc atttcaatat tgtcttcttt
    18841 ggccatctaa tcctctgggg tatcactacc gtaatttgaa ccggcaagat aatgccgaag
    18901 ttctgtaaat aagtaaagat ttgcgcgcta aatcgcaaca aacaggttcg gcacattact
    18961 ccgaaaacac acggctaagc cgcaccaaaa gcgcaacgta taagggagcg gtgagataaa
    19021 cgatgggcgt tacctgacgc gaaaaaatcc ttatcggcag cggggtaatg agcgtaacca
    19081 actctgcgac cgcaattata acactctggg gagaaatgtg ccgaaaacat tcattcttgt
    19141 ggtgaaaaca agcatcgtgg tacccagaaa ttattcggca atcgtccgag gcgcatttga
    19201 ttgagataat taaggtaatc ccggcgggga atttcgcagg caccaagcga tgctgtgtga
    19261 tcgttaagga cctggcagtc gataagctta ccgccatgac cgataaattc ctcacagaat
    19321 accagaagcg ccgttttaga cgcattttcc atccggctga acatggactc gccacaaaat
    19381 agcgttccct gggccacgcc gtacataccg ccgacaagct catcttcacg ccagacttca
    19441 atggagtggg catggccgag ttcgtgaagg cgatggtagg cttcgaccac gccacgcgtg
    19501 atccaggttc cttcttcgcg atcgctggca cagccttcaa tgacctgacc aaaagcgtaa
    19561 ttcatcgtga cacgataggg cgagcgttta tgaaatcgct tcatactacg gctgatatgc
    19621 agtgattctg gccatagcac cgcgcgggga tcgggcgacc accagaggat ggggtcgcct
    19681 ggagaaaacc acggaaaaat accacgctgg taagccatta acaggcgcgc agggctaaga
    19741 tcgcccccaa gtgccagcag gccgttaggc tcacgtaatg cgccttccgg ggaagggaag
    19801 gctattgaat ggcgagaaag ctgaaccagg cgcatgaccg caaaactcca cgcaagtcgg
    19861 atcgttcaat aatagcttac aaaccctgct tgaactggta ataacgcccc tgtctggcaa
    19921 gcagctctgc gtgagtacct tgctcaataa tttgcccgtt gtccatcact attatttgtt
    19981 ggaaacgaga gagtccgcga agtcgatggg tgaccattaa caccgttttc tcacgcatca
    20041 tttctgcaag caattcaagg atctggcttt cggttgtggc atctaagcct tcggtaggtt
    20101 catccagcaa caccagtggc gcatcatgta acagcgcacg ggcgatagcc agacggcgca
    20161 gttcaccacc ggagagctgg cgtccgcctt cacctaacca actgttgaga cctgcatcct
    20221 cgagcagctt ctccagacca acgcgacgca agctctccgc cagagcctca tcactactgc
    20281 caggcgaggc gagtaacaga ttatcacgca gcgtggcgct aaacagatgc actcgctgag
    20341 gaacaacgct gatggtctgt cgtagagccg cttcattcag gctggctatg gggctatcgt
    20401 taagcaaaat ctctccctgt tgcgggtccc aagcgcgggt cagcagttgt aacagtgttg
    20461 atttgccgca tccggttcgc ccgagaatcg caatatgttc cccggcgttt acctgaagag
    20521 aaatcccttt aagtgcctgt tgggattgct ccggataagt gaactgaaca tcccgtaatg
    20581 tcagcgaaac gcgatcggca acacgagttt gggtatcagg gaaggtgact tccggttttt
    20641 gatccgttaa atcagtgatc cgaacggcag aggcaatgac ttgccccaga tgctgaaatg
    20701 cacccgttac tggtgccagt gcttcaaacg cggctaacgc gcagaagaca aacagggcaa
    20761 ttaacgcgcc gggttgagca ttgccgccaa cgccgccaga cgccatccac agcatcagga
    20821 tcaccgctaa cgcgccaatg agcagcatta tcgcttgcga caatgcggtc agttcagatt
    20881 gacggcgttg cgcttccagc cattgaattt ctgtattctc tagttgcgtg cgataacgat
    20941 cgctggcgcc aaaaatggtc agctcagctt gcccttgcag ccaggccgtc agttgttggc
    21001 gatactgtcc gcgaagatga gtcagatttt gcccggtgct ttttcccgca cgataaaaca
    21061 gcggtggcat caggaaaagc gtcagtaaca taatgccgcc cagcgtaaag gcgagggtga
    21121 aatcaaggaa acttaacccg attgtcacca ccataatcac cacaaaagcg cccaccagcg
    21181 gcgagataac gcgcagataa agatgatcga gcgtatcaac atccgccacc acgcgattga
    21241 gcaattcgcc ctgacgatag cgcgccagtc cggcagggga gaggggcagc aatttgctga
    21301 aggtgtaaat gcgcagatgc tgcaacacgc ggaaagtcgc gtcgtgactt accagacgtt
    21361 caaaataacg cccggcagta cgggtgattg ctgcgccacg cacgcccgca gcgggtagca
    21421 tatagttgaa gctgtacagt ccggcaaccc ccgcaaccgc tgaggccgag aggaaccagc
    21481 cggaaagtgt caacagaccg atactggcga gcagcgtgac aattgccagc acgataccaa
    21541 gacttaacat ccatttatga cgtttataca gtgccagata gggtagcaaa gcgcgcattt
    21601 aaatctcctc ctgacgatgg gccagtaatg tggcgaatgg gccaccagcc acacttaatt
    21661 ccgcgtaacg tccttgctca ataatccggc catcctgcat aacccaaatg acatcccagt
    21721 cagcaagatc ttctaactgg tgggtgacca ttaacgttgt ctggcgcaga gaggcggcat
    21781 tcagcgcctc cattacgcgc tgttcactgt gagcatcaag gctggcagcg ggttcatcca
    21841 acagtaatag cgaacaggga tttagtaacg cacgggccac cgccacgcgc tgcgcctgcc
    21901 ccacggaaag gcgggcagcc tggtcgccaa caggtgtatc aacgccttgt ggcaggagcg
    21961 gtagaaactc gctgacccag gcgttatcca gcgctgcttg taattcttgt tcgctggcat
    22021 caggtcgcgc cagtagtacg ttatcccgca atgttgctgc cggtaattgt gggttttgcc
    22081 caacccagga gagatgttta cgccatgatt ctggtgataa atcgcgtaat tctatcccgt
    22141 tgattcgtaa tgatccctga tatgagagaa aaccagaaag cgcgttcagc agtgagcttt
    22201 tacctgaacc gctgcgacca accaacactg cacgttggcc tgctggcaaa gtaaagttca
    22261 gcggtccggc cagcgtttta ccttccggcg acgtgataaa cagatcctcg gcctcaatgg
    22321 tcaccggatc ggtcgatgct aattccacct cgccacgctg cggatgggcg agcggggttt
    22381 ccataaacgt tttcagactg tcagctgcac caacagcctg ggctttagca tgataaaacg
    22441 taccgagatc gcgcaatggc tggaaaaact ctggcgcaag gatcagggcc agaaaacccg
    22501 cagccagcgt cacaccggta tcgtagtgac caaaatccag ctcgccgaga taggaaaaac
    22561 caaagtagac cgccaccaga gcaattgaca gcgaggtaaa aaattcgaga atgccggagg
    22621 ataaaaacgc cagccgtagc acttccattg tccgttggcg gaaatcttcc gaagcagaac
    22681 gaatactttc aatttcagct tcaccacgac caaaaatacg caatgtttcc atgccgcgca
    22741 ggcgatcgag gaaatgccca cttaagcgag caagagcgag aaagttacgc cggttagcat
    22801 cggcagcccc catcccaacc agcgccataa acaacggaat taacggtgct gtgcccagca
    22861 gaatgagcgc cgcagcccag ttagagggga agatcgccac cacaatcagc aacggcaccg
    22921 acactgccag cgccatttgc ggcaggtagc gtgcatagta atcatgcata tcgtcaattt
    22981 gctcgagtac caacgtcgcc cagctccccg caggtttacc ctgaatccac gctggccctg
    23041 cttgttgcag acggtcgaga acctgacggc ggatggcaaa gcggatatgc tgcccggcgt
    23101 gataacccac ccgttcgcgt aaccagacca cccatgcgcg cagtacaaag gtcagaacca
    23161 gtaacgtaaa gggaagcagc agggcttcac ggggaatatt ctccataatc atatgttgca
    23221 gaattcgcgc catgaaccag gcctgggcaa tgatcaatat gccgcttaca aagcccagca
    23281 gacgagaaat attcagccaa cgttgggaga tgacgctttg ctgttttaac cagcgggtta
    23341 actctttttg acgggattta ttcattgcac gcttagcagg tgagttatca gaattatttg
    23401 cagagcaatg ttacaacggg gaaaaaataa aggcgaccca tagtcgcatg gtgtcgcctt
    23461 ctttactttt gttactgatt tgtaaaatta ttttgcgtca gctaaaccat cgaggtagcg
    23521 ttccgcatca agtgctgcca tgcagcctgt accggccgaa gtaatggcct gacgataaat
    23581 atgatccatc acgtcacccg cggcaaagac gccaggaatg ctggtctggg tggcattacc
    23641 atgaataccc gactgcactt tgatgtagcc gttttccagt tccagctgcc cttcgaaaat
    23701 ggcagtattc gggctgtgac cgatagcaac aaacagacca gcaacgtcga gtgactcgat
    23761 gttatcgctg ttttgcgtat cgcgcagacg aacgccagtg acacccattt gatcgccggt
    23821 cacttcttcc agcgtacggt tggtgtgcag aatgatgttg ccgttctcca ctttatccat
    23881 cagacgctta ataaggattt tttccgcgcg gaaaccgtca cggcggtgaa taagatgcac
    23941 ttccgaagcg atgttagaca gatacagcgc ctcttcaacc gcggtattgc cgccgccgat
    24001 gaccgcaact ttctggttgc gatagaagaa accgtcgcag gttgcacaag cagaaacccc
    24061 acggccttta aaggcttctt cagagggcag gccgagatag cgtgcagaag ctccggtggc
    24121 aataatcagc gcgtcgcaag tgtattcgcc gttatcgcca ttcagacgga acggacggtt
    24181 ttgcagatcc accttgttga tatgatcaaa aatgatctca gtttcaaact tggtggcatg
    24241 ttcgtgcatg cgctccatta ataacggacc ggtcagatcg tttggatcgc caggccagtt
    24301 ttccacttcc gtggtggtgg tcagttggcc gcctttttcc atgccggtaa tcagcacagg
    24361 ttgcaggttg gcgcgcgccg cgtagacagc agcggtgtat cccgccgggc ctgaacccag
    24421 gataagcagt ttactgtgtt tggtcgtgcc catgagatcc ccatagttgt tggcagacaa
    24481 tgggcaggat tgtagggaat ttacagacgt aaaaaaagag tatgacgatt ttgttaacaa
    24541 tttgtgcaat cggcagcatc gataagcagg tcaaattctc ccgtcattat cacctctgct
    24601 acttaaattt cccgctttat aagccgatta aatgatgaat aaacgcccct gttaatgaat
    24661 atctggcatg ttgtactaaa aatcgatgtt ttgctttgac aatcccctgg tgttttgcga
    24721 aaacattcga ggaagaaaaa aacagtattc ttatatgcgc ataaccatgc atgtaaatac
    24781 catgtttacc gtgctagtga aatctacgta tggcgtggac agacgccgtt cgtgatgtcg
    24841 atagctgcca caaggcaacg gtcttctcac cgtagaccca ggcattgcgc gccgtgaatc
    24901 ttcatgattt cggtctatcg tgacgggtag cgactctgaa cagtgatgtt tcagggtcag
    24961 acaggagtag ggaaggaata cagagagaca ataataatgg tagatagcaa gaagcgccct
    25021 ggcaaagatc tcgaccgtat cgatcgtaac attcttaatg agttgcaaaa ggatgggcgt
    25081 atttctaacg tcgagctttc taaacgtgtg ggactttccc caacgccgtg ccttgagcgt
    25141 gtgcgtcggc tggaaagaca agggtttatt cagggctata cggcgctgct gaacccccat
    25201 tatctggatg catcacttct ggtattcgtt gagattactc tgaatcgtgg cgcaccggat
    25261 gtgtttgaac aattcaatac cgctgtacaa aaacttgaag aaattcagga gtgtcattta
    25321 gtatctggtg atttcgacta cctgttgaaa acacgcgtgc cggatatgtc agcttaccgt
    25381 aagttgctgg gggaaaccct gctgcgtctg cctggcgtca atgacacacg gacatacgtt
    25441 gttatggaag aagtcaagca gagtaatcgt ctggttatta agacgcgcta acacggaaca
    25501 ggtgcaaaat cggcgtattt tgattacact cctgttaatc catacagcaa cagtactggg
    25561 gtaacctggt actgttgtcc gttttagcat cgggcaggaa aagcctgtaa cctggagagc
    25621 ctttcttgag ccaggaatac actgaagaca aagaagtcac attgacaaag ttaagtagcg
    25681 gccgccgcct tctggaggcg ttgctgatcc ttattgtcct gtttgccgtc tggttgatgg
    25741 ctgccttact aagctttaac ccttcggacc ccagctggtc gcaaacggcc tggcatgaac
    25801 ctatccataa tttaggtggg atgcccggtg cgtggttggc agatacgctg ttctttattt
    25861 ttggcgtgat ggcttacacc attcccgtca ttattgtcgg cggttgttgg tttgcctggc
    25921 gtcatcagtc cagcgacgaa tacattgatt attttgccgt ttcgctacgc atcattggcg
    25981 ttttggcgct catccttacc tcctgtggtc tggcggcaat caacgctgac gatatctggt
    26041 attttgcctc cggtggcgtc attggcagct tactaagtac tacgctacaa ccactgctac
    26101 acagtagcgg gggaactatt gcgctgctct gcgtttgggc tgcgggcctg acgctgttca
    26161 ccggttggtc atgggtgacc attgctgaaa aactcggcgg ctggatttta aacattctca
    26221 ccttcgccag taatcgtacc cgtcgcgatg atacctgggt cgatgaagat gaatatgaag
    26281 atgacgaaga gtatgaagat gaaaaccacg gcaaacagca tgaatcacgc cgtgcccgta
    26341 ttcttcgcgg cgcgctagcg cgtcgtaaac ggttggcgga aaaattcatt aatccgatgg
    26401 ggcggcaaac agacgctgcg ttgttctccg gtaagcggat ggatgatgac gaagagatta
    26461 cctacactgc acgcggtgtg gctgccgacc cggacgacgt cctattttcg ggcaatcgtg
    26521 caacgcagcc agaatatgac gaatacgatc cattattaaa cggtgcgcca attaccgaac
    26581 ctgtcgctgt agcagctgct gctaccacgg cgacacaaag ctgggctgcg ccggttgaac
    26641 ctgtgactca gacgccgcct gttgcctctg ttgatgttcc accctcgcaa cctacagtag
    26701 cctggcagcc tgtaccgggt ccacaaacgg gagagccggt tattgctcct gcaccggaag
    26761 gttacccaca gcagtcacaa tatgcgcagc ctgcagtgca atataatgag ccgctgcaac
    26821 aaccagtaca gccgcagcag ccgtattatg cacctgcagc tgaacaacct gcgcaacaac
    26881 cttactacgc tcctgcggcg gaacaacctg ttcagcaacc gtattacgca actgcgccgg
    26941 aacagcctgc acaacagccg tattacgccc ctgcgccaga acaaccggtg gcaggtaacg
    27001 cctggcaagc cgaagagcag caatccactt ttgctccaca gtctacatac cagactgagc
    27061 aaacttatca gcaaccagcc gctcaggagc cgttgtacca acagccgcaa tccgttgaac
    27121 agcagcctgt tgtggagcct gaacccgttg tagaagagac aaaacccgcg cgtccgccgc
    27181 tttactattt tgaagaagtg gaagagaagc gagcccgtga acgtgaacaa cttgcggcct
    27241 ggtatcaacc gattccagaa ccggttaaag aaccagaacc gatcaaatct tcgttgaaag
    27301 cgccttctgt tgcagcagta cctccagtag aagccgctgc cgctgtttct ccgctggcat
    27361 ctggcgtgaa aaaagcgaca ctggcgacgg gggctgccgc aaccgttgcc gcgccagtct
    27421 tcagtctggc aaatagcggt ggaccgcgtc cgcaactcaa agaggggatt ggcccgcagt
    27481 tgccacgacc gaaacgtatc cgcgtgccaa ctcgtcgtga actggcgtct tacggtatta
    27541 agctgccctc acagcgtgcg gcggaagaga aagcccgtga agcccagcgc aatcagtatg
    27601 attctggcga tcagtataac gatgatgaaa tcgatgcgat gcagcaggat gaactggcac
    27661 gtcagttcgc ccagacacag cagcaacgct atggcgaaca gtatcaacat gatgtgcccg
    27721 taaacgcaga agatgcagat gctgcggcag aggctgaact ggctcgtcag tttgcgcaaa
    27781 ctcaacaaca acgttattcc ggcgaacaac cggctggggc gaatccgttc tcgctggatg
    27841 attttgaatt ttcgccaatg aaagcgttgc tggatgatgg tccacacgaa ccgttgttta
    27901 cgccaattgt tgaacctgta cagcagccgc aacaaccggt tgcaccgcag cagcagtatc
    27961 agcagccgca acaaccagtt ccgccacagc agcaatatca gcagccgcaa cagccggttg
    28021 cgccgcagcc gcagtatcag cagccacaac agcaggttgc gccgcagcca caatatcagc
    28081 agccgcaaca accggttgcg ccacagccgc aatatcagca gccgcaacaa ccagttgcgc
    28141 cgcagccgca gtatcaacag ccgcaacaac cggttgcacc gcagcagcag gataccctgc
    28201 ttcatccgct gttgatgcgt aatggcgaca gccgtccgtt gcataaaccg acgacgccgc
    28261 tgccttctct ggatttgctg acaccgccgc cgagcgaagt ggagccggta gatacctttg
    28321 cgcttgaaca aatggctcgc ctggtggaag cgcgtctggc tgatttccgt attaaagccg
    28381 atgtcgtcaa ttactctccg gggccggtta tcactcgctt tgaacttaac ctggctcctg
    28441 gcgtaaaagc ggcgcgcatt tctaacttgt cacgggacct tgcccgttca ctttcgacgg
    28501 tggcggtgcg tgtcgttgaa gttattcctg gcaaacccta tgtaggtctg gagttaccaa
    28561 ataaaaaacg acaaaccgtt tatctgcgcg aagttttgga taacgccaaa ttccgcgata
    28621 atccgtcgcc attaaccgtg gtgctgggta aagatatcgc cggtgagccg gtggttgccg
    28681 atctggcgaa aatgccgcac ttgttggttg cggggactac cggttccggt aaatctgtcg
    28741 gtgtgaacgc gatgatcctg agcatgcttt ataaagcaca gccagaagat gtgcgtttca
    28801 tcatgatcga cccgaaaatg ctggagcttt cggtttatga aggcattccg catctgttaa
    28861 cggaagtcgt tactgatatg aaagatgccg ccaacgcgct gcgctggtgt gttaacgaga
    28921 tggagcgtcg ttataaactg atgtctgcgc tgggtgtgcg taatctggcg ggttataacg
    28981 aaaaaattgc tgaagccgat cgcatgatgc gtccgattcc agacccgtac tggaagccgg
    29041 gtgacagtat ggatgcccag catccggtgc tgaaaaaaga accatacatt gtggtgttgg
    29101 ttgacgaatt tgccgacctg atgatgacgg taggtaaaaa agtggaagag ctgatagcac
    29161 gtctggcgca aaaagcccgt gccgcgggta tccacctcgt actggcaact cagcgtccat
    29221 cggttgatgt tattactggt ctgattaaag cgaatattcc gacccgtatc gcctttaccg
    29281 tatccagtaa gattgactca cgtaccattc ttgatcaggc tggcgcggaa tcactgctgg
    29341 ggatggggga tatgctctac tctgggccga actccacgtt gccggtacgt gtccatggtg
    29401 cttttgttcg cgatcaggaa gttcatgccg tggtgcagga ctggaaagcg cgtggtcgcc
    29461 cacagtatgt tgatggcatc acttccgaca gtgaaagcga aggtggcgcg ggcggtttcg
    29521 atggcgctga agaactggat ccgttgttcg atcaggcggt gcagtttgtc actgaaaaac
    29581 gcaaagcgtc aatttctggc gtacagcgtc agttccgcat tggttataac cgtgcagcgc
    29641 gtattatcga acagatggaa gcgcagggga ttgtcagcga acaggggcac aacggtaatc
    29701 gtgaagtgct ggccccaccg ccgtttgact aattaatgca ttgccggata aggcgcggta
    29761 gcgtcgcatc cggcactcta tcaactgaaa attcagtatt ttcttctttc ctcaagctga
    29821 ttattagcct ggaatagaga gtagagggaa ctcccgatcg ggagtgacgt aatttgagga
    29881 ataatgatga aaaaaattgc catcacctgt gcattactct caagcttagt agcaagcagc
    29941 gtttgggctg atgccgcaag cgatctgaaa agccgcctgg ataaagtcag cagcttccac
    30001 gccagcttca cacaaaaagt gactgacggt agcggcgcgg cggtgcagga aggtcagggc
    30061 gatctgtggg tgaaacgtcc aaacttattc aactggcata tgacacaacc tgatgaaagc
    30121 attctggttt ctgacggtaa aacactgtgg ttctataacc cgttcgttga gcaagctacg
    30181 gcaacctggc tgaaagatgc caccggtaat acgccgttta tgctgattgc ccgcaaccag
    30241 tccagcgact ggcagcagta caatatcaaa cagaatggcg atgactttgt cctgacgccg
    30301 aaagccagca atggcaatct gaagcagttc accattaacg tgggacgtga tggcacaatc
    30361 catcagttta gcgcggtgga gcaggacgat cagcgcagca gttatcaact gaaatcccag
    30421 caaaatgggg ctgtggatgc agcgaaattt accttcaccc cgccgcaagg cgtcacggta
    30481 gatgatcaac gtaagtagag gcacctgagt gagcaatctg tcgctcgatt tttcggataa
    30541 tacttttcaa cctctggccg cgcgtatgcg gccagaaaat ttagcacagt atatcggcca
    30601 gcaacatttg ctggctgcgg ggaagccgtt gccgcgcgct atcgaagccg ggcatttaca
    30661 ttctatgatc ctctgggggc caccgggtac cggcaaaaca accctcgctg aagtgattgc
    30721 ccgctatgcg aacgctgatg tggaacgtat ttctgccgtc acctctggcg tgaaagaaat
    30781 tcgcgaggcg atcgagcgcg cccggcaaaa tcgcaatgca ggtcgccgca ctattctttt
    30841 tgttgatgaa gttcaccgtt tcaacaaaag ccagcaggat gcatttctgc cacatattga
    30901 agacggcacc atcactttta ttggcgcaac cactgaaaac ccgtcgtttg agcttaattc
    30961 ggcactgctt tcccgtgccc gtgtctatct attgaaatcc ctgagtacag aggatattga
    31021 gcaagtacta actcaggcga tggaagacaa aacccgtggc tatggtggtc aggatattgt
    31081 tctgccagat gaaacacgac gcgccattgc tgaactggtg aatggcgacg cgcgccgggc
    31141 gttaaatacg ctggaaatga tggcggatat ggccgaagtc gatgatagcg gtaagcgggt
    31201 cctgaagcct gaattactga ccgaaatcgc aggtgaacgt agcgcccgct ttgataacaa
    31261 aggcgatcgc ttttacgatc tgatttccgc actgcataag tcggtacgtg gtagcgcacc
    31321 cgatgcggcg ctctactggt atgcgcgaat tattaccgcc ggtggcgatc cgttatatgt
    31381 cgcgcgtcgt tgcctggcaa ttgcttccga agatgtcggc aatgccgacc cacgggcgat
    31441 gcaggtggca attgcggcct gggattgctt tactcgcgtt ggcccggcgg aaggtgaacg
    31501 cgccattgct caggcgattg tttacctagc ctgcgcgcca aaaagcaacg ctgtctacac
    31561 tgcgtttaaa gccgcgctgg cggatgctcg cgaacgtccg gattatgacg tgccggttca
    31621 tttgcgtaat gcgccgacga aattaatgaa ggaaatgggc tacgggcagg aatatcgtta
    31681 cgctcatgat gaagcaaacg cttatgctgc cggtgaggtt tacttcccgc cggaaatagc
    31741 acaaacacgc tattatttcc cgacaaacag gggccttgaa ggcaagattg gcgaaaagct
    31801 cgcctggctg gctgaacagg atcaaaatag ccccataaaa cgctaccgtt aatgttatcg
    31861 ttgcggtaat gttgttactg tatccctgtg gtcgcaggct gtggccacat ctcccattta
    31921 attcgataag cacaggataa gcatgctcga tcccaatctg ctgcgtaatg agccagacgc
    31981 agtcgctgaa aaactggcac gccggggctt taagctggat gtagataagc tgggcgctct
    32041 tgaagagcgt cgtaaagtat tgcaggtcaa aacggaaaac ctgcaagcgg agcgtaactc
    32101 ccgatcgaaa tccattggcc aggcgaaagc gcgcggggaa gatatcgagc ctttacgtct
    32161 ggaagtgaac aaactgggcg aagagctgga tgcagcaaaa gccgagctgg atgctttaca
    32221 ggctgaaatt cgcgatatcg cgctgaccat ccctaacctg cctgcagatg aagtgccggt
    32281 aggtaaagac gaaaatgaca acgtcgaagt cagccgctgg ggcaccccgc gtgagtttga
    32341 ctttgaagtt cgtgaccatg tgacgctggg tgaaatgcac tctggcctcg actttgcagc
    32401 cgcagttaag ctgaccggtt cccgctttgt ggtaatgaaa gggcagattg ctcgcatgca
    32461 ccgcgcactg tcgcagttta tgctggatct gcataccgaa cagcatggct acagtgagaa
    32521 ctatgttccg tacctggtta accaggacac gctgtacggt acgggtcagc tgccgaaatt
    32581 tgctggcgat ctgttccata ctcgtccgct ggaagaagaa gcagacacca gtaactatgc
    32641 gctgatccca acggcagaag ttccgctgac taacctggtg cgcggtgaaa tcatcgatga
    32701 agatgatctg ccaattaaga tgaccgccca caccccatgc ttccgttctg aagccggttc
    32761 atatggtcgt gacacccgtg gtctgatccg tatgcaccag ttcgacaaag ttgaaatggt
    32821 gcagatcgtg cgcccggaag actcaatggc ggcgctggaa gagatgactg gtcatgcaga
    32881 aaaagtcctg cagttgctgg gcctgccgta ccgtaaaatc atcctttgca ctggcgacat
    32941 gggctttggc gcttgcaaaa cttacgacct ggaagtatgg atcccggcac agaacaccta
    33001 ccgtgagatc tcttcctgct ccaacgtttg ggatttccag gcacgtcgta tgcaggcacg
    33061 ttgtcgcagc aagtcggaca agaaaacccg tctggttcat accctgaacg gttctggtct
    33121 ggctgttggt cgtacgctgg ttgcggttat ggaaaactat cagcaggctg atggtcgtat
    33181 tgaagtacca gaagttctgc gtccgtatat gaacggactg gaatatattg gctaataccc
    33241 aatttttctg aatctaaaaa gcgcctgcgg gcgctttttt tgtctccctt tgataccgaa
    33301 caataattac tcctcactta cacgtaatac tactttcgag tgaaaatcta cctatctctt
    33361 tgattttcaa attattcgat gtatacaagc ctatatagcg aactgctata gaaataatta
    33421 cacaatacgg tttgttactg gaatcaatcg tgagcaagct tgagtgagcc attatgaaaa
    33481 cgaaaatccc tgatgcggta ttggctgctg aggtgagtcg ccgtggtttg gtaaaaacga
    33541 cagcgatcgg cggcctggca atggccagca gcgcattaac attacctttt agtcggattg
    33601 cgcacgctgt cgatagcgcc attccaacaa aatcagacga aaaggttatc tggagcgcct
    33661 gtacagttaa ctgtggtagt cgctgcccgc tacgtatgca cgtcgtggac ggtgaaatca
    33721 aatatgtcga aacggacaat accggcgatg acaattacga cggcctgcac caggttcgcg
    33781 cctgtctgcg tgggcgttcc atgcgtcgcc gtgtctacaa tccggaccgc ctgaaatatc
    33841 cgatgaaacg agtcggggcg cgcggtgaag gcaaattcga gcgcattagc tgggaagaag
    33901 cctacgacat catcgcgacc aatatgcagc gcctgatcaa agagtacggc aacgagtcta
    33961 tctatctgaa ctatggcacc ggtacgctgg gcggcaccat gacccgctcc tggccgccgg
    34021 gaaataccct ggtcgcgcgc ctgatgaact gctgcggcgg ttatcttaac cattacggtg
    34081 actactcttc tgcgcaaatt gcggaaggtt tgaactatac ctacggcggc tgggcagatg
    34141 gtaacagccc gtcggatatc gaaaacagta aattggtagt gctgtttggt aataacccag
    34201 gcgaaacgcg aatgagtggc ggtggggtga cttactatct tgaacaggca cgccagaaat
    34261 ctaatgcccg catgatcatc atcgatccgc gctataccga caccggtgcc gggcgcgaag
    34321 atgagtggat ccctattcgt ccaggaacag atgccgcact ggttaacggt ctggcgtatg
    34381 tcatgatcac cgaaaacctg gtggatcagg cattccttga taaatattgc gttggctacg
    34441 atgagaaaac cctgcccgct agcgcgccga aaaatggcca ctataaagct tatattctgg
    34501 gtgaagggcc agatggcgtg gctaaaacac cgcaatgggc ctcgcaaatc accggtatcc
    34561 cggcagagaa aatcattcaa ctggcacggg aaatcggcag taccaaaccg gcgtttatca
    34621 gccagggatg ggggccacag cgccacgcca acggcgaaat cgcgactcgc gctatctcga
    34681 tgctggcgat tctgaccggt aacgtcggca ttaacggcgg taacaccggc gcgcgtgaag
    34741 gttcctatag cctgccgttt gtccgtatgc cgaccctcga aaacccgatc cagaccagca
    34801 tttctatgtt tatgtggacc gatgccattg aacgtggccc ggaaatgacg gcgctacgtg
    34861 atggtgtgcg cgggaaagat aagctggatg tgccgatcaa aatgatctgg aactatgccg
    34921 gtaattgcct gattaaccag cattctgaaa tcaaccgtac ccatgaaatc cttcaggatg
    34981 ataagaagtg cgagctgatt gtggttatcg actgccacat gacctcatcg gcgaaatatg
    35041 ctgacatcct gctgcctgac tgcaccgctt ccgaacagat ggactttgca ctggatgcat
    35101 cctgcgggaa tatgtcttac gtgattttca acgatcaggt gattaaaccg cgctttgaat
    35161 gtaaaaccat ctatgaaatg accagcgaac tggcaaaacg tcttggcgtt gagcaacagt
    35221 ttactgaagg ccgtacacag gaagagtgga tgcggcatct gtatgcccag tcgcgggaag
    35281 cgattcctga actgccaacg tttgaagagt tccgcaagca ggggatcttt aaaaagcgcg
    35341 acccacaagg gcatcacgtt gcttataaag ccttccgtga agatccgcag gcaaacccac
    35401 tgactacgcc atcgggcaaa attgagattt attcgcaggc gctggctgac attgccgcta
    35461 cctgggaatt gccagaaggc gatgtgatcg atccactgcc gatctacacg ccgggctttg
    35521 aaagttatca ggatccgctg aacaaacagt atccgctgca gcttacgggt ttccactata
    35581 aatctcgcgt tcactcaact tacggcaacg ttgatgtgct gaaagcggct tgccgtcagg
    35641 aaatgtggat caacccgctt gatgcgcaaa aacgcggtat caacaacggc gataaagtca
    35701 ggatctttaa cgatcgtggt gaagttcata ttgaggcgaa agtgacgcca cgaatgatgc
    35761 cgggtgtggt cgcactgggt gaaggtgcct ggtatgaccc ggatgcaaaa cgtgtcgata
    35821 agggcggttg tattaacgta ctgaccactc aacgtccgtc tcctctcgct aaggggaatc
    35881 cgtcacatac aaaccttgtt caggttgaaa aggtgtaagg agtaaccgat gacaacccag
    35941 tatggatttt ttattgattc cagccgttgc accggttgca aaacctgcga gctggcctgt
    36001 aaagactaca aagatttgac gccagaagtc agcttccgcc gcatttatga atatgctggc
    36061 ggcgactggc aggaagataa cggtgtctgg caccagaacg tgtttgccta ctatctgtcg
    36121 atttcatgta accactgcga agatccggct tgtactaaag tctgcccgag cggtgcgatg
    36181 cataaacgcg aagatggttt tgttgtggtc gatgaagatg tgtgcattgg ctgccgctac
    36241 tgccatatgg cttgcccgta tggcgcaccg caatataacg aaacgaaagg ccatatgacc
    36301 aaatgcgatg gttgttatga ccgtgttgcg gagggtaaaa agccgatctg tgttgaatct
    36361 tgtccgctgc gggcgctgga tttcggccct atcgacgagc tgcgtaaaaa acatggcgat
    36421 ctggcggccg ttgcgccgtt gccgcgagct cactttacca aaccgaatat tgtgatcaaa
    36481 cccaatgcca atagccgccc gaccggggat accactggct atctggcaaa cccgaaggag
    36541 gtgtgagatg ggaagtggat ggcatgaatg gccgctgatg atcttcacgg tcttcgggca
    36601 atgtgtagca ggtggtttta tcgttctggc tttggcgctg ctcaaaggcg acctgcgagc
    36661 agaagcccag cagcgtgtta tcgcctgcat gtttggttta tgggtgctga tgggcattgg
    36721 ctttatcgcc tctatgctcc atcttggttc accaatgcgc gcttttaact cgctcaaccg
    36781 ggtaggggct tcagcactca gtaacgaaat cgccagcggt tcgatctttt ttgccgtagg
    36841 cggcatcggc tggttgctgg caatgctgaa aaagctgtca ccggcattgc gtacgctgtg
    36901 gctgatagtg acgatggttc ttggcgtcat ctttgtctgg atgatggtgc gtgtgtataa
    36961 cagcattgat accgttccga cctggtacag catctggacg ccgatgggct tcttcctgac
    37021 gatgtttatg ggcggcccgt tactgggtta cctgttgttg agtctggctg gcgtcgatgg
    37081 ctgggcgatg cgtctgctgc cagcaatttc tgtactggca ctggtagtga gtggcgtggt
    37141 gtcagtgatg cagggcgcag agctggcgac tattcatagt tctgtgcagc aggccgcagc
    37201 gctggtgccg gactatggtg cgctgatgtc ctggcggatc gtgcttttgg ccgttgccct
    37261 gtgcttgtgg attgcaccac agctaaaagg ttatcagcct gcggttccgc tactatccgt
    37321 ctcattcatt ctgcttctgg caggggaatt aatcggtcgc ggcgtattct atggtttgca
    37381 tatgaccgtg gggatggccg tcgcaagcta atcagtaaca accggggttt cggccccggt
    37441 tctcttttat ttctgcttcg ttaacgtgtc ataactggtc atcaaattac gataatcagg
    37501 aatgtggtta gagaacaacg tcgccagtcc ttcaatatca ttacgccagt cgcgatgcag
    37561 ctcgcacgcc acgccgaacc atgtcatcaa ttgcgcacca gcttgcgaca tgcgatccca
    37621 tgccgaatgc cgggtaattt cattaaaggt ccctgacgcg tcagtcacga caaagacgtc
    37681 aaacccttct tcaatggctg aaagcgccgg gaatgccacg caaacttcgg ttaccacacc
    37741 ggcaataatt aactgttttt tacctgtcgc tttgacagct tttacaaaat cttcgttatc
    37801 ccaggcgtta atatttcccg ggcgagcaat gtaaggtgta tcggggaatt gtgctttcag
    37861 ttctggaact aatgggccgt taggaccggt ttcaaaactg gtggtaagaa tagttggtaa
    37921 gttgaaatac ttagccaggt cacccagcgc cagcacgata tttttaaact tatcaggttc
    37981 gatatcccgt acaagggaaa gtaaaccagc ctggtgatca acaagcaaaa cggcagcatc
    38041 atttttatca agacgaacat acggtttggt catcctcttc tcctttcgag agtcagtggt
    38101 acgtctgaaa tcgatcagac gcgatgcatt gctctgaaag catagacggg aaatatgagt
    38161 ttgctgtgac catgaaattt ttcgactgaa cgaagcgtat gaaatttgtg ttagttcaat
    38221 aaaaacaatc agatgagact aatcatctct tcagcattag cgttatgaat gagtaatata
    38281 aatcgataat accgccgctg ggatatcgcg tattttcacc cattgacaat gtttttggcg
    38341 gtggcatgat gcgcatgaaa tttgaacttc ctcacggttt taattcatgt ccacgtatac
    38401 ccggcctgtc atgcttttgc tgtctggcct gcttttgttg actctggcga ttgcggtgtt
    38461 aaatacactc gtgccgcttt ggctcgccca ggaacacatg tccacatggc aggtaggcgt
    38521 tgtcagctca tcctatttta ccggcaacct tgtcggtaca ttgctgacag ggtatgtcat
    38581 taagcgcatt ggctttaacc gcagctatta tctggcctcc ttcatttttg ccgctggctg
    38641 tgccggcctt ggcctgatga ttggattctg gagctggttg gcttggcgtt ttgtcgcggg
    38701 cgtcggctgt gccatgattt gggtggttgt tgagagcgcg ctgatgtgca gtgggacgtc
    38761 acgtaaccgt gggcgtttgc ttgctgcgta tatgatggtt tattacgtgg gaacgttttt
    38821 aggccagtta ctggtcagca aagtttcaac cgagctgatg tccgtattgc cgtgggttac
    38881 aggtttgacg ttggcaggga tcttaccgct gttgtttacg cgtgtgctga atcagcaggc
    38941 tgaaaaccat gattcgacgt caattacgtc aatgctaaaa ctccgtcagg cgcggcttgg
    39001 cgtgaatggc tgcattatct caggaatcgt tctgggatct ctatatggcc tgatgccgct
    39061 gtacctcaat cacaaagggg tgagcaatgc cagcattggt ttctggatgg cggtactggt
    39121 cagtgcgggt atccttggac aatggccgat tggacgtctg gcggataagt ttggtcgact
    39181 gctggtgttg cgtgttcagg tctttgtcgt cattctcggc agtatcgcga tgcttagcca
    39241 ggcggcgatg gccccagcgt tattcatcct cggtgccgct ggctttacgc tatatccggt
    39301 ggcgatggca tgggcttgcg agaaagttga acatcatcaa ctggtggcga tgaaccaggc
    39361 cttactgttg agctatactg tgggaagtct gcttggcccg tcatttaccg ctatgctaat
    39421 gcagaatttc tccgataatt tattgtttat catgatcgcc agcgtatcgt ttatctattt
    39481 gctgatgctg ctgcgcaacg ccggtcatac gccgaaaccc gttgctcacg tgtaaatgaa
    39541 ttcaagcaga gtgtgaactt actgtttcac actctgcttt tttgtttctt ctatctgact
    39601 tgctttattc caaattttat tcgtttaaaa ataaaatgtg cagcaggtta taattttgca
    39661 tttcgctatt tccgcacttc ttatttgccg cgcataatcc ctcgttttac agatgcccct
    39721 ttaattttgg cgaaggattt gtctatggct gggaatgttc aggaaaaaca gctgcgatgg
    39781 tacaacattg cgctgatgtc ttttatcact gtctggggtt ttggcaacgt tgttaataac
    39841 tacgccaacc aggggctggt ggttgttttt tcatgggtgt ttatctttgc gctctatttc
    39901 acaccttatg cgctaattgt tggtcagtta ggctcgacct tcaaagatgg gaggggcggg
    39961 gtcagtacct ggattaaaca cacgatggga cccggactgg cttacctcgc cgcgtggacc
    40021 tactgggtag tgcatattcc ctatctggca caaaaacccc aggcaatact gattgcgctc
    40081 ggttgggcga tgaaaggcga cggttcgtta atcaaagaat attcagtcgt agcgttacag
    40141 gggttaacgc tggtgctgtt tatcttcttt atgtgggttg cttcacgcgg tatgaaatcg
    40201 ctgaaaatcg tcggttctgt ggcagggatt gcgatgtttg ttatgtcgct cctgtatgtg
    40261 gcgatggcgg taaccgcgcc tgcaattact gaagtgcata ttgcgaccac aaacattacc
    40321 tgggaaacgt tcattcctca tatcgacttt acctacatta ccactatttc aatgctggtt
    40381 ttcgcggttg gcggagcaga gaagatttct ccttacgtta atcaaacgcg caacccagga
    40441 aaagaatttc caaaagggat gttatgcctg gcggtgatgg ttgcggtttg tgccattctg
    40501 ggctcgctgg cgatggggat gatgtttgat tcgcgtaata tcccggatga cttaatgacc
    40561 aacggtcagt attacgcctt tcagaagctg ggcgagtatt acaacatggg taatacttta
    40621 atggtgattt acgccattgc gaataccctg ggacaagtag cggcgctggt attctcgatt
    40681 gatgccccgc ttaaagtgct attaggtgat gctgacagca aatatattcc agccagttta
    40741 tgtcgtacca acgcttctgg tacgcccgtt aatggctatt ttctgaccct ggtactggtg
    40801 gcgattctga ttatgctgcc gactctcggc attggtgata tgaacaatct ctataaatgg
    40861 ctgttgaacc ttaattcggt agtgatgccg ctgcgttatt tatgggtatt tgttgcattt
    40921 attgcagtcg ttcgcctggc gcagaaatat aaaccagaat atgtctttat tcgtaataag
    40981 cctctggcaa tgaccgtcgg gatttggtgt tttgccttta ccgcctttgc ctgtttgacg
    41041 gggatcttcc cgaaaatgga agccttcact gcagagtgga ccttccagtt ggcgctgaat
    41101 gttgcaacgc cgtttgtgct ggtaggatta ggactgattt tcccgctgct ggcgcgtaaa
    41161 gcgaatagta aataattatt gtggtcggtg acgctgactc tggcagatct gacccgtttt
    41221 tacataatcg agaaatgcgc gcaagccgca ggacatatac tggcggtttg gataatagat
    41281 ctggaagcct ggacgctctg tgctccagtc ttccagtaca cattctaaac gaccagtatc
    41341 aaggtactcc ttgatctgtt catagagcag atacccgata ccagcaccca tcaaaacggc
    41401 ctccagttca gcatcaacat catccagaat gatatttcct gcgacagcaa tttccagctc
    41461 ctttgcaaat tgccagtgga aaggtttgcc gctgggataa cgaaaaacaa cacattgatg
    41521 attaagcaga tcatgaggat ggcggggttt gccatatctt gcaaagtact ctggcgttgc
    41581 tgctacacat aatttcactg gcgggccgat cgccacgctg atcatatctt tttcgacgat
    41641 gccactaagt cgaacgccag cgtcaaagcc ctgttggacg atatcgacaa gagagtcgtc
    41701 agttgtgagc tcgactttaa tatcaggata ttcacgtgtg aaaccaacca gtaaaggcat
    41761 taaaaatata cgtgcagcta cccgggcggc attgattttg agcgttcctg tcggtgtcag
    41821 acgaaaatca ttcatctcat ctaacatgat ctgaatttcg tcgaaagctg ggcgcaatcg
    41881 ttcatagaga ttagaacccg cttccgttaa cgatacgctt cgggttgtcc gattgaataa
    41941 gcgaatttta agacgttgtt ccagtgtttt aatactatgg ctaatggcgg acgaggataa
    42001 gcctaactca tcgcccgctg cacgaaagct ttgattacgg gccacggcaa agaaagtggc
    42061 aaagtcagac atattcatcc gcattagtga aaatccttca ttaactcatc ctgaatcatc
    42121 acgtttacag gattatgccg cctgcgtaaa gtgcgctcca gaacttaacg tggaggtaaa
    42181 attatgcagt ctgaacgtat ttatttggta tgggcccatc ctcgtcatga ttcattgacc
    42241 gcacatattg ctgatgcgat ccatcagcgg gcaatggagc ggaaaataca ggtgacggaa
    42301 ctcgatttat atcggcgtaa tttcaaccca gtgatgacgc cggaagatga accagactgg
    42361 aagaatatgg ataaacgtta ttctccagag gttcatcagc tttattcaga gctgcttgaa
    42421 catgacacgt tagtggtggt ttttcctctc tggtggtaca gcttcccggc aatgctaaaa
    42481 ggatatattg acagagtatg gaataatggg ctggcttatg gagatgggca caaattacca
    42541 ttcaataaag ttcgttgggt ggcgctggtt ggaggagaca aagaatcatt tgtccagatg
    42601 ggctgggaaa aaaatataag cgattattta aaaaatatgt gcagttatct tggtattgaa
    42661 gatgccgatg tcactttctt gtgtaataca gtggtattcg atggggaaga acttcacgcg
    42721 agctattatc agtcgttatt atctcaggta cgggatatgg tagatgcact ataagatgtg
    42781 ttaaaaacgc tgtagcagaa tgaagcgcgg aataaaaaag cggcaactca ataaagttgc
    42841 cgctttacgg ggaaattaga acattacctt atgaccgtac tgctcaagaa tgcctttcac
    42901 gcgttccatg gtctctttct tcggtggttt aacaccgtcg agtttgtact cttcacccat
    42961 tgccacccat ttgtgtttgc ccagctcgtg gtaggggaga agctcgattt tctcaacgtt
    43021 gcccatatca cgggtaaatt caccgaggcg atgcgctgaa tcgtcatcgt cagaccagcc
    43081 tgggacaaca acgtagcgga tccacacctt cacatttttg ttcgccagat atttagcgaa
    43141 ctccagcgtg cggtggttgg aaactccaac cagattttgg tggatctcgt cgttcatctg
    43201 tttgagatcg agcattacca ggtcggttac ttccagcagt tcatcaatca ccggatcgta
    43261 acgacgaaca aaaccgttgg tgtccagaca ggtatgaatg ccttcttttt tgcaggcgcg
    43321 gaaccagtca cgaacaaact cagcttgcag gattgcttca ccgccggatg cggtaacgcc
    43381 gccgccggaa gcgttcataa agtggcgata ggtcaccact tccttcatca aatcttcaac
    43441 ggtaacttct ttaccgccat gcgtgtccca ggtgtcgcgg ttatgacaat acaggcagcg
    43501 catcaggcag ccctggaaaa aggtgataaa gcgaatacct gggccgtcta cggttccaca
    43561 ggattcaaag gagtgaatgc gaccaataac tgacattgcg gtgtttctcc agatgtggcc
    43621 catctgaggc cgtgttggtg cgcagctcga aggctacgtc gagtctgttt tggcagtcac
    43681 cttaaagtat agatagctga caaaaaaggc tctcgtgcta aaaaaggccc cactttcgtg
    43741 gagcctttat tgtacgcttt ttactgtacg atttcagtca aatctaatta catagattga
    43801 gtgaaggtac gagtaataac gtcctgctgc tgttctttag tcagcgagtt gaaacgtact
    43861 gcgtagccag atacacggat ggtcagctgc ggatattttt ccgggttttc catcgcgtcg
    43921 agcagcattt cacggttcat cacgttaacg ttcaggtgct gaccaccttc gatggatgct
    43981 tcgtggtgga agtaaccatc catcagacct gccaggttgg tcttacgaac ttcgtcgtct
    44041 ttacccagtg cgttcggaac gatagagaag gtgtaggaga taccatcttt agcgtaagca
    44101 aacggcagtt tagcaacgga agtcagagac gctacagcac ctttctggtc acgaccgtgc
    44161 atcgggttag cacccggtcc gaacggcgcg ccagcacgac gaccgtctgg ggtgttacca
    44221 gttttcttac catacacaac gttagaagtg atggtcagaa cagactgagt cgggatagcg
    44281 tcacggtagg tgtgcagttt ctgaattttc ttcatgaaac gttctaccag gtcaacagcc
    44341 aggtcatcta cacgcggatc attgttacca aactgcgggt attcgccttc gatttcgaag
    44401 tcgatagcca gaccgtcttc gtcacgaatc ggtttaactt tcgcatattt gattgcagac
    44461 agggagtcag cagcaacgga cagaccagcg ataccacacg ccatggtgcg gataacgtca
    44521 cggtcgtgca gcgccatcag agaggcttcg tagctgtact tgtcgtgcat gtagtggatg
    44581 atgttcagtg cagtgatgta ctgtttagcc agccagtcca tgaagtgatc catgcgctcc
    44641 atcacttcat cgtagttcag gagatcgcct ttgatcggtt cagacttcgg accaacctgc
    44701 attttcagtt tttcgtcaac gccgccgttg attgcgtaca gcatggtttt cgccaggttt
    44761 gcacgcgcac cgaagaactg catttgttta ccaacgatca tcgggcttac gcagcaagcg
    44821 atagcatagt catcgttgtt gaagtccgga cgcatcaggt catcgttctc atactgcaga
    44881 gaagaggtgt cgatggacac tttagcggcg aatttcttga agttcagcgg cagtttttca
    44941 gaccacagaa tggtcatgtt cggttccgga gacggaccca tggtgtacag ggtgttcagg
    45001 aaacggaagc tgtttttggt aaccagggta cgaccgtcga ggcccatacc accgatagat
    45061 tcggttgccc agatcgggtc gccagagaac agttcatcgt attccggagt acgcaggaag
    45121 cgaaccatac gcagtttcat gaccaggtgg tcaaccattt cctgcgcttc ttgttcggtg
    45181 atcttgccag ctttcaggtc acgttcgatg tacacatcca ggaaggtgga ggtacgaccg
    45241 aaggacattg cagcaccgtt ctgagactta acagcagcca ggtagccgaa gtaagtccac
    45301 tggatagctt cctgagcgtt ggtagccgga ccagagatgt cgtagccgta tttcgcagcc
    45361 atttctttca tctgacccag agcgcggtgc tgttcagcga tttcttcgcg cagacggata
    45421 gtctgttcca ggtttacgcc gttttccaga tcagtctgca gagaggtgaa ctgagcgtat
    45481 ttgtctttca tcaggtagtc gataccgtac agcgcaacgc gacggtagtc accgatgata
    45541 cggccacggc cataagcatc tggcagaccg gtcagaacac cggatttacg gcaacgcagg
    45601 atgtccggag tgtaaacgtc gaacacgccc tggttgtgag ttttacggta ttcagtgaag
    45661 atttttttga tcatcgggtc cagttcgcgg ttgtacgctt tgcaggaacc ttcgatcatt
    45721 ttgataccac cgaacgggat aagagcacgt ttcagcggag cttcagtctg cagaccaaca
    45781 actttttcca acgctttgtt gatgtaacca gcgtcgtgag aggtgatggt agaagcaaca
    45841 gcggtgtcaa agtcaactgg cgcgtgagtg cggttttcca gtttaacgcc ttccattact
    45901 ttgtcccaca gggtggtggt cgcttcagta gcgccagcca ggaaggactc gtcaccctcg
    45961 tacggagtgt agtttttctg aatgaagtca cggacgttta cttcattctg ccagtcacct
    46021 ttggtaaaac cttcccaggc tgtggctaac ttttcattaa gctcggacat gtaacaccta
    46081 ccttcttaag tggatttttt atttactgcg tacttcgaca accattaatg gtgatcgttt
    46141 tcacgcaggt aaatgaccca gtatgtcaac ccaaccaaca aaccaccgcc gataatatta
    46201 ccgatcgtaa ccggaatcag gttatcagtg atgaagttca tcacggtcag gtgagaaaaa
    46261 ttttccggtg cagaaccgac agcggtccag aattccggag atgcgaagtc gcggattaca
    46321 atacccatcg ggatcataaa catgtttgcg atactgtgct caaaaccgct ggcaacaaac
    46381 atcgcgaccg gcagcaccat aatgaacgct ttgtccatca ggctacggcc agaataactc
    46441 atccatactg ccagacatac catcaggttt gccaggatac caaggcagac ggcctcaata
    46501 aaagtatggt gcactttgtg gtcggcggtt tgtaggacgt ttagtcccca ttgaccattt
    46561 gcggtcatat actcgccgga aagccacatt aaaagtacaa acagcagtgc gccgaccagg
    46621 ttgccaaaat agacatttag ccagtttttc gccaactgac cccaggtgat gcgcccactc
    46681 gccttagcaa caacaatcaa cactgtggaa gtaaagagat cggctccgca gacaacacaa
    46741 agaatcagcc ccagagagaa gcaaatgccg ccaaccagtt ttgccatgcc gaagggcatt
    46801 gtgcctgtgc cagtggttgc tgtgatatag aagacgaatg cgattgagat gaaaacaccg
    46861 gcggtaatcg ccagatagaa agtcttaagc ggatgtttcg ttgctttata gacacccgcc
    46921 tcttcggcca ctttggccat tgcagcagga agtaaaagat caaaagggtt gtcagctttc
    46981 acactaactc tctctttatt aagtcggcga cgagatacta acaaagcatt atagatgaga
    47041 aattgatata gatcatatct cgcctggctt ataggcccgt aactcgcatg gtttttatgc
    47101 aaatacggag taaatatttg attatccaaa taaaaataaa ttttaaaaat taacaaatga
    47161 gttgaatttt ttccgcatcc tccgctaaaa cagttaatta aaagggagca tcaggcgaat
    47221 aaagtaac
//
LOCUS       DBANUU010000021        46754 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000021 DBANUU010000000
VERSION     DBANUU010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.021"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(271..813)
                     /gene="ycfP"
                     /locus_tag="ACIE68_001141"
     CDS             complement(271..813)
                     /gene="ycfP"
                     /locus_tag="ACIE68_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFX5907489.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(836..1861)
                     /gene="nagZ"
                     /locus_tag="ACIE68_001142"
     CDS             complement(836..1861)
                     /gene="nagZ"
                     /locus_tag="ACIE68_001142"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFX5907490.1"
                     /translation="MGPVMLDVKGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDSRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(1872..2696)
                     /gene="thiK"
                     /locus_tag="ACIE68_001143"
     CDS             complement(1872..2696)
                     /gene="thiK"
                     /locus_tag="ACIE68_001143"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFX5907491.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2677..3318)
                     /gene="lpoB"
                     /locus_tag="ACIE68_001144"
     CDS             complement(2677..3318)
                     /gene="lpoB"
                     /locus_tag="ACIE68_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFX5907492.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3332..3709)
                     /gene="ycfL"
                     /locus_tag="ACIE68_001145"
     CDS             complement(3332..3709)
                     /gene="ycfL"
                     /locus_tag="ACIE68_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFX5907493.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPITVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3712..4071)
                     /gene="hinT"
                     /locus_tag="ACIE68_001146"
     CDS             complement(3712..4071)
                     /gene="hinT"
                     /locus_tag="ACIE68_001146"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFX5907494.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4405..6594
                     /gene="fhuE"
                     /locus_tag="ACIE68_001147"
     CDS             4405..6594
                     /gene="fhuE"
                     /locus_tag="ACIE68_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFX5907495.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGIYEL
                     FGRQHNLMFGGSYSKQNNCYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(6654..8087)
                     /gene="ptsG"
                     /locus_tag="ACIE68_001148"
     CDS             complement(6654..8087)
                     /gene="ptsG"
                     /locus_tag="ACIE68_001148"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFX5907496.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8382..9179)
                     /gene="ycfH"
                     /locus_tag="ACIE68_001149"
     CDS             complement(8382..9179)
                     /gene="ycfH"
                     /locus_tag="ACIE68_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFX5907497.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVNFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDSSRLQSIR"
     gene            complement(9190..10194)
                     /gene="holB"
                     /locus_tag="ACIE68_001150"
     CDS             complement(9190..10194)
                     /gene="holB"
                     /locus_tag="ACIE68_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFX5907498.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10191..10832)
                     /gene="tmk"
                     /locus_tag="ACIE68_001151"
     CDS             complement(10191..10832)
                     /gene="tmk"
                     /locus_tag="ACIE68_001151"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFX5907499.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10822..11844)
                     /gene="yceG"
                     /locus_tag="ACIE68_001152"
     CDS             complement(10822..11844)
                     /gene="yceG"
                     /locus_tag="ACIE68_001152"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFX5907500.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11847..12656)
                     /gene="pabC"
                     /locus_tag="ACIE68_001153"
     CDS             complement(11847..12656)
                     /gene="pabC"
                     /locus_tag="ACIE68_001153"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFX5907501.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLHDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12776..14017)
                     /gene="fabF"
                     /locus_tag="ACIE68_001154"
     CDS             complement(12776..14017)
                     /gene="fabF"
                     /locus_tag="ACIE68_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFX5907502.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14105..14341)
                     /gene="acpP"
                     /locus_tag="ACIE68_001155"
     CDS             complement(14105..14341)
                     /gene="acpP"
                     /locus_tag="ACIE68_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFX5907503.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14552..15286)
                     /gene="fabG"
                     /locus_tag="ACIE68_001156"
     CDS             complement(14552..15286)
                     /gene="fabG"
                     /locus_tag="ACIE68_001156"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFX5907504.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEASYITGETLHVNGGMYMV"
     gene            complement(15299..16228)
                     /gene="fabD"
                     /locus_tag="ACIE68_001157"
     CDS             complement(15299..16228)
                     /gene="fabD"
                     /locus_tag="ACIE68_001157"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFX5907505.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16244..17197)
                     /gene="fabH"
                     /locus_tag="ACIE68_001158"
     CDS             complement(16244..17197)
                     /gene="fabH"
                     /locus_tag="ACIE68_001158"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFX5907506.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLTASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17265..18335)
                     /gene="plsX"
                     /locus_tag="ACIE68_001159"
     CDS             complement(17265..18335)
                     /gene="plsX"
                     /locus_tag="ACIE68_001159"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFX5907507.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18416..18589)
                     /gene="rpmF"
                     /locus_tag="ACIE68_001160"
     CDS             complement(18416..18589)
                     /gene="rpmF"
                     /locus_tag="ACIE68_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFX5907508.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18641..19162)
                     /gene="yceD"
                     /locus_tag="ACIE68_001161"
     CDS             complement(18641..19162)
                     /gene="yceD"
                     /locus_tag="ACIE68_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFX5907509.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPVQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19361..19945
                     /gene="yceF"
                     /locus_tag="ACIE68_001162"
     CDS             19361..19945
                     /gene="yceF"
                     /locus_tag="ACIE68_001162"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFX5907510.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTL
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20057..21016)
                     /gene="rluC"
                     /locus_tag="ACIE68_001163"
     CDS             complement(20057..21016)
                     /gene="rluC"
                     /locus_tag="ACIE68_001163"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFX5907511.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21589..24774
                     /gene="rne"
                     /locus_tag="ACIE68_001164"
     CDS             21589..24774
                     /gene="rne"
                     /locus_tag="ACIE68_001164"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFX5907512.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQKVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(24971..25924)
                     /gene="flgL"
                     /locus_tag="ACIE68_001165"
     CDS             complement(24971..25924)
                     /gene="flgL"
                     /locus_tag="ACIE68_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFX5907513.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGFRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(25936..27579)
                     /gene="flgK"
                     /locus_tag="ACIE68_001166"
     CDS             complement(25936..27579)
                     /gene="flgK"
                     /locus_tag="ACIE68_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFX5907514.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNTGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27645..28586)
                     /gene="flgJ"
                     /locus_tag="ACIE68_001167"
     CDS             complement(27645..28586)
                     /gene="flgJ"
                     /locus_tag="ACIE68_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFX5907515.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEEPTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28586..29683)
                     /gene="flgI"
                     /locus_tag="ACIE68_001168"
     CDS             complement(28586..29683)
                     /gene="flgI"
                     /locus_tag="ACIE68_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFX5907516.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29695..30393)
                     /gene="flgH"
                     /locus_tag="ACIE68_001169"
     CDS             complement(29695..30393)
                     /gene="flgH"
                     /locus_tag="ACIE68_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFX5907517.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30446..31228)
                     /gene="flgG"
                     /locus_tag="ACIE68_001170"
     CDS             complement(30446..31228)
                     /gene="flgG"
                     /locus_tag="ACIE68_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFX5907518.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31400..32155)
                     /gene="flgF"
                     /locus_tag="ACIE68_001171"
     CDS             complement(31400..32155)
                     /gene="flgF"
                     /locus_tag="ACIE68_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFX5907519.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32175..33380)
                     /gene="flgE"
                     /locus_tag="ACIE68_001172"
     CDS             complement(32175..33380)
                     /gene="flgE"
                     /locus_tag="ACIE68_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFX5907520.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(33405..34100)
                     /gene="flgD"
                     /locus_tag="ACIE68_001173"
     CDS             complement(33405..34100)
                     /gene="flgD"
                     /locus_tag="ACIE68_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFX5907521.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34112..34516)
                     /gene="flgC"
                     /locus_tag="ACIE68_001174"
     CDS             complement(34112..34516)
                     /gene="flgC"
                     /locus_tag="ACIE68_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFX5907522.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34520..34936)
                     /gene="flgB"
                     /locus_tag="ACIE68_001175"
     CDS             complement(34520..34936)
                     /gene="flgB"
                     /locus_tag="ACIE68_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFX5907523.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35091..35750
                     /gene="flgA"
                     /locus_tag="ACIE68_001176"
     CDS             35091..35750
                     /gene="flgA"
                     /locus_tag="ACIE68_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFX5907524.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSAQLAGISDEVR
                     VSIRTAPSLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            35826..36119
                     /gene="flgM"
                     /locus_tag="ACIE68_001177"
     CDS             35826..36119
                     /gene="flgM"
                     /locus_tag="ACIE68_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFX5907525.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36124..36540
                     /gene="flgN"
                     /locus_tag="ACIE68_001178"
     CDS             36124..36540
                     /gene="flgN"
                     /locus_tag="ACIE68_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFX5907526.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSADISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(36580..38115)
                     /gene="murJ"
                     /locus_tag="ACIE68_001179"
     CDS             complement(36580..38115)
                     /gene="murJ"
                     /locus_tag="ACIE68_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFX5907527.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38225..39148)
                     /gene="yceM"
                     /locus_tag="ACIE68_001180"
     CDS             complement(38225..39148)
                     /gene="yceM"
                     /locus_tag="ACIE68_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFX5907528.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39150..39797)
                     /gene="yceH"
                     /locus_tag="ACIE68_001181"
     CDS             complement(39150..39797)
                     /gene="yceH"
                     /locus_tag="ACIE68_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFX5907529.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPSVTDMSSAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39808..40392)
                     /gene="rimJ"
                     /locus_tag="ACIE68_001182"
     CDS             complement(39808..40392)
                     /gene="rimJ"
                     /locus_tag="ACIE68_001182"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFX5907530.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40628..41836
                     /gene="mdtH"
                     /locus_tag="ACIE68_001183"
     CDS             40628..41836
                     /gene="mdtH"
                     /locus_tag="ACIE68_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFX5907531.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41900..42547
                     /gene="grxB"
                     /locus_tag="ACIE68_001184"
     CDS             41900..42547
                     /gene="grxB"
                     /locus_tag="ACIE68_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFX5907532.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42681..43241
                     /gene="yceB"
                     /locus_tag="ACIE68_001185"
     CDS             42681..43241
                     /gene="yceB"
                     /locus_tag="ACIE68_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFX5907533.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43347..44393
                     /gene="pyrC"
                     /locus_tag="ACIE68_001186"
     CDS             43347..44393
                     /gene="pyrC"
                     /locus_tag="ACIE68_001186"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFX5907534.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44467..44712
                     /gene="dinI"
                     /locus_tag="ACIE68_001187"
     CDS             44467..44712
                     /gene="dinI"
                     /locus_tag="ACIE68_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFX5907535.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            45002..45256
                     /gene="bssS"
                     /locus_tag="ACIE68_001188"
     CDS             45002..45256
                     /gene="bssS"
                     /locus_tag="ACIE68_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFX5907536.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            45371..46489
                     /gene="solA"
                     /locus_tag="ACIE68_001189"
     CDS             45371..46489
                     /gene="solA"
                     /locus_tag="ACIE68_001189"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFX5907537.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIHHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            46537..46650
                     /locus_tag="ACIE68_001190"
     CDS             46537..46650
                     /locus_tag="ACIE68_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFX5907538.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT        11335 a        12573 c        11624 g        11222 t
ORIGIN      
        1 tgatgttaat caagttttgt tgctgaaaag ataggcgact ttaccgggtg agatcaaaaa
       61 atagagttta ttgtttatta tttgagcaaa ttcggaagag taatataggg tgaagcgtat
      121 atcgagggcg tttggtcgtc ctgataagat gcgcagcatc gcatcaggca ttgatgctcg
      181 actgtcggat gcggcgtgga cgccttatcc gacttacaga attcggagcg catcaggtac
      241 cggtaaggtt acctgatgcg acgagggcat ttacccgagg gttttgaacg ctttaatgcg
      301 ctgtaaatgc ggggagatat tcttgaattt gtgcgtctgc tcttcgtccc agacaatctc
      361 gtaataatga tgcaactctt cagatgtccg ctggctgtta agtgcttcat cattacgcga
      421 caaaataacc agacaacgat cgcgattctt ctcgcggaag ttggtcacac acttagtggc
      481 aatatcggca tactcttccg ggcgatcaat cttgccttcc atgttctcat aagggaacaa
      541 attagggttg aagatcacct ggcggatatc gcaaagaaaa ccaatccgtt ccgcccagta
      601 tccgcccagg ccaacgccgc aaattagcgg acgctcgtca acgttcagtt gcaacatttt
      661 gtccacttct ttaagcagat gctgcatatc atgtttcgga tgccgcgtac tgtagcttat
      721 caagcgtaca tccgggtcaa taaactgcaa ttgtaagact ttctcgtggt tacccggact
      781 gttagagtca aaaccgtgta aatagataat catcgcttcc tcacataagc cagggttagt
      841 gacctgcttt ctcttcctgc cagcgttcgt gtaactgatt cagacgggtg ctgatcgctt
      901 tccagcgagc cgagtccatc agttcctgtc gcgaaaatga acctttatga tacaaacgtg
      961 taacacgttc tgccttgatc ggtgacagat tatctaacac gctgacggcc cctttacgat
     1021 tattgcagac caggatcata tcgcaacccg catccagtga tgcctgcccg cgttcggcat
     1081 aactgcccat aatcgcggca ccttccatcg ataaatcgtc agagaaaatc acgccgtcaa
     1141 aacccagttc ctgacgcaaa acggttttca gccagtaggg agaaccgctc gccggacgcg
     1201 aatcaacatc actgtagatc acatgcgcag gcataatggc gtcgagttta ttttcgcgga
     1261 ttaaggaact gaagaccgac atatctttag cgcgaatctc cgcttgtgga cgtggatcgc
     1321 acggtgtttc tttgtgtgag tctgccgtta ctgcaccgtg tcctgggaag tgtttcccgg
     1381 tcgttttcat tccggcttca tgcataccat caataaaccg gctggcaatt gccagggctt
     1441 tttgtggatc ggcatgataa gaacgctcgc caatcgccgc gctgatatgc ccgacatcca
     1501 gcactggcgc aaagctgata tcaatatcca tagcgatcat ttcgctggcc atcagccaac
     1561 ccgcctcttg cgccagtttg ccgccctctt ccattcctga cagcgcagcg aatgattgcg
     1621 ccgctggcaa gcgggtaaaa ccttcacgaa aacgctgcac gcgtccacct tcctgatcta
     1681 ccgccaccac cagatgattg cgcgaagctg cgcggatctg gcgcaccagt tcacgtaact
     1741 gggcaggatc atgatagtta cgcgtaaaga gaatcagccc tcccaccagc ggatgcgcaa
     1801 gtatttcacg ctcttccgcg tccagttcgt aacctttgac atccaacatt actggaccca
     1861 cactgctctc cttattgttt tattaatagc tgccgccagg tgtcatcggc cagcctgata
     1921 aattgttgat cgccggtttg tcgccagcgg tactcaaacc accctgcttt gagcatcagc
     1981 agccagggaa accatcgcct gacctgacgc cataattgcg ccggataaat cttcgcgcga
     2041 gtggcatagt cattgaccaa ttgccggtgc tgttcagtat tttccaccca caccgccgcc
     2101 agttccagcg cgatatcacc atctcctgca tactcccagt cgatgagttt taaccctgac
     2161 gcgctatgca ctaaatttcc ggcgtggaca tccatatgca atggacttaa gcgtaaaggc
     2221 cgtggttccc gcgctttgcg cagacgtttt aacattcgca gccaacccac tgtccgccgc
     2281 gccggatcgc tttgctgcca gtacagttcc agtaacggca acagcgttat tcgccagcca
     2341 aaacgtggtt gttgatgtag ataatacagc aagcctgcca gttcgttggt atccggcaaa
     2401 tacgttttta ccgcgccggg cagatagtcg actaccatcc agtcacggag atataaatgc
     2461 ggcttcggtg caatgcatgc gggtagttgt gataaagccc gatactggcg caagaacgcg
     2521 gactgcggcg catcaggatc gtgcggctga cgcacaacaa aacgctggcc ctggtgttca
     2581 atgagaaaac tcccgccact aagcccacta ttaaacgtcg tgacgggatg aaactgcggg
     2641 aaaaagcgcg acagcaattc gtcgcgcgtg atgggattat tgctgcgaaa cggcaccttt
     2701 acctgaccag ataatttcgc ccgtctgcac cagcatcagc tgcatttgta gggtcggagc
     2761 gttaacgttg ccagaggcgc tggagtatag cacgtaatga gcgccgacat tgcgggcaat
     2821 gcctatggct ttactacggg tacctaaact gtcctgcggc gacaaaccta actgttgctt
     2881 agccatcgac agctgctggg cggaaaccag ggtaaattta ccgttattag ccagcgcatt
     2941 tcgcagcgtt tcggtcgctt ctgcggcatt cagcgaaccg ttagtacggt tgttaacgct
     3001 atcaaccagc aggacgctac ccgcagtcac cccgtcagcc ccaagcatct tactgaccat
     3061 cggctgcatt gcgccattcc agtcataatg gcgaatatgc ggcgcaggcg gtgcagtttg
     3121 atcttcgtgc tcaattgggc ctggctgctg cgggatcgtc ggcaccgagg gcactgtggg
     3181 gacaggctgt tgtggctcgg ctggttgttc cggcgctggt ttcacttctt ctaccggtgc
     3241 aggttcacgt tgccccacac acccggcgag aaacatcgcc agcgcggtaa tcaaggcgta
     3301 gcgactcatt tttgtcatca agattcaccc cttacaaata tagataaagt ctgactttgt
     3361 gcgcccccag aaaattggcg ctgccgtaca gcgttaccgc cgaatgtgcg ggaatggtaa
     3421 cgctgcgtgg cctttccaga ggatgcatct ccagccctct ggcgtcatac cagtaaaaac
     3481 gataatgaac ggtaatgggt tcttgccttt cgttataaag cgttgaggat gctgaaggtt
     3541 gaatatcaga cgtcgaaagg acgggctttt ctgcactgat gcccgcagcc aataaagatg
     3601 actccatcac cagcgattgc tcatcattca ccggaatttc cggatgtgaa cgacagccca
     3661 ccagcagtaa caacgccaga gacaccagcc caaagcatcc ttttctcatc gttacagacc
     3721 tttatgcgcc agcattggtc ccagcggacg gccacccaac aagtgcatat ggatgtggta
     3781 aacctcttgt ccgccatggc ggttggtatt catgatcaga cgatagccat cttcggcaat
     3841 accttcttgc tcagcaattt ttgccgctac ggtgatcatg cgtcccagcg cctgctcatg
     3901 ctcagctgac acgtcgttca cagtcggaat gaggatattc ggaatgatca gaatatgcgt
     3961 tggcgcttgc ggcgaaatat cgcgaaacgc cgttaccaga tcatcctggt agacgatatc
     4021 ggaggggatc tcacgacgaa taattttgct gaatatagtt tcttctgcca cgacattttc
     4081 ctttttcaaa tagccatgcg ttgcgccatg ctacgccgcg ccacactgcg agtatagagt
     4141 atggtcgagt taaacctgct ctttcaacct taactcacga tttcttaagc aaaaaatcgt
     4201 tttgctgccg tatatatcgc cattattccc atttctgcct gcgaatgcgc tgaaaagaca
     4261 ttctctgttt ggtttacacg acaaatatga atacgtatat ttctcatttg catttacaag
     4321 caaaattatt cgcacactaa atataaccgc tcatcaccct cctccggcat gagcctgacg
     4381 acaacataaa ccaagagatt tcaaatgctt tcaacacaat ttaacaggga taatcaatat
     4441 caagccatca ccaaaccctc actacttgcc ggttgcatag cattggcact attaccttct
     4501 gccgcttttg ctgcaccagc cactgaagaa acggtgattg ttgagggttc agccacagct
     4561 ccagatgatg gcgaaaatga ttacagcgta acgtctacct ctgcgggtac caaaatgcag
     4621 atgactcaac gtgatattcc tcagtcggtc actattgtta gccagcagcg gatggaagat
     4681 cagcagttac aaacgctggg cgaagtgatg gaaaacacgc tggggatcag caaaagtcag
     4741 gcggattccg atcgtgctct ttattattcc cgcggattcc agatcgataa ctatatggtt
     4801 gatggtatcc ccacctattt tgaatcgcgc tggaatctgg gcgacgcact ttctgatatg
     4861 gcactgtttg aacgcgtaga agtagtgcgt ggcgcgacag gactcatgac cgggacgggt
     4921 aatccatctg cggcaattaa tatggttcga aaacacgcga ccagtcgtga atttaaaggc
     4981 gatgtctcgg cggaatacgg tagctggaac aaagaacggt atgtggcgga tttacaaagc
     5041 ccactcaccg aagacggtaa aatccgcgcg cgaattgtcg gcggctacca gaataacgac
     5101 tcatggctgg accgctacaa cagtgaaaag accttcttct cgggcattgt cgatgctgat
     5161 ttaggcgatc ttacgacgct ttcagccggt tacgaatatc agcgcattga tgttaatagc
     5221 cctacctggg gcggtttacc gcgctggaat actgatggca gcagcaacag ttacgatcgt
     5281 gcacgcagta ccgcacctga ctgggcgtac aacgataaag agatcaacaa ggtctttatg
     5341 accctgaagc agcggtttgc tgatacctgg caagcgacac tgaatgccac ccactctgaa
     5401 gtcgaatttg acagcaaaat gatgtatgtc gatgcctatg taaacaaagc ggatggtatg
     5461 ctggttgggc catacagtaa ttatggacct ggctttgatt atgtcggcgg caccggttgg
     5521 aacagtggca aacgtaaagt tgatgcgctg gatttgttcg ctgacggtat ttatgaattg
     5581 tttggtcgtc agcacaatct aatgtttggt ggcagttaca gcaaacaaaa caattgttac
     5641 ttcagttcat gggccaacat cttcccggat gaaattggca gtttctacaa ctttaatggc
     5701 aatttcccac aaaccgactg gtcaccacag agcctggcac aggacgatac cacacatatg
     5761 aaatcgttat atgctgccac tcgagtcacc cttgccgatc cgctgcatct gatcctcggc
     5821 gcacgttata ccaactggcg ggttgatacg ctgacttaca gcatggagaa aaaccacacc
     5881 acgccttacg ctggtctggt gtttgacatc aatgacaact ggtcgaccta cgccagctat
     5941 acctctattt tccagccgca aaatgatcgt gacagttcag gcaaatatct ggctccaatc
     6001 accggtaaca actacgagct gggtctgaaa tcggactgga tgaatagccg tctgaccacc
     6061 acgttagcca tcttccgtat tgagcaggat aatgtcgctc agtccaccgg tacacctatc
     6121 cccggcagca acggcgaaac cgcctataaa gcggtggatg ggacagtcag taaaggggtg
     6181 gaatttgaac tcaacggcgc aattaccgac aactggcagc tgacatttgg cgcaacgcgc
     6241 tatattgcag aggataacga aggaaacgcc gttaatccta atctgccacg cactacggtt
     6301 aaaatgttca ccagctatcg gttgcctgtc atgccagagt tgacagtcgg cggtggtgtt
     6361 aactggcaaa atcgcgtgta taccgacacc gtgacaccgt atggcacctt ccgcgccgag
     6421 caaggtagct acgcgctggt ggatctcttc acccgctacc aggtgacgaa aaacttctcg
     6481 ttacagggga acgtcaataa cctgttcgac aaaacctacg ataccaacgt ggaaggttct
     6541 atcgtctacg gcgcaccgcg taatttcagc attaccggca cgtatcaatt ctgaataaca
     6601 cctgtaaaaa aggcagccat ctggctgcct tagtctcccc aacgtcttac agattagtgg
     6661 ttacggatgt actcatccat ctcggttttc aggttgtcgg atttagtacc gaaaatcgcc
     6721 tgaacaccag aaccagcaac cactacgccc gctgcgccca gtttcttcag gccagcctga
     6781 tccactttag acacatcagc aacgctgacg cgcagacggg taatacatgc gtcgaggtta
     6841 gtaatgtttt ctttaccacc aaatgcagca accagagccg gtgccatttc gctggtacct
     6901 gtcgcttttg catcttcagt cgcgtcttca cgacccggcg ttttcagatc cagtgcttta
     6961 atcagcacgc ggaagatggt gtagtaaaca atcgcataac cgataccgac gatcgggaac
     7021 agccacagtt tgctgctgtt accagacaga acgatgaagt cgatcagacc gtgcgagaac
     7081 gacgtaccgt cacgcatccc cagaagaata cagattggga atgccaggcc tgccagaatc
     7141 gcgtggatga tgtacaggat cggcgcaacg aacatgaagg agaactcgat cggctcggtg
     7201 ataccggtca ggaacgaggt cagcgccgcg gagatcataa taccgcccac tttcgcgcgg
     7261 ttttctggtt tagcagagtg ccagatagca attgcggcag ctggcagacc gtacattttg
     7321 aacaggaagc caccagacag tttacccgca gtcgggtcac ccgccatata acgcggaatg
     7381 tcgccgtgga aaacctgacc tgctgcgttg gtgtattcac caatctgcat ctggaaaggt
     7441 acgttccaga tgtggtgcag accaaacggt accaggcaac gttcgatgaa accgtaaatg
     7501 ccaaacgcaa ctaccgggtt ctggtaagca gcccactgag agaaggtctg gattgcagaa
     7561 ccaatcggcg gccaaatgaa ggacagcaca acgccagtaa agatggcagc caggccagaa
     7621 atgatcggca caaagcgttt accggcaaag aagccaagat actcaggcag cttaatacgg
     7681 tagaaacggt taaacatgta cgctgcgatc gcaccggaga taatccctcc gagtacgcca
     7741 gtatccgcca ggtgtttaga ggcgatttct tcagcaggta aatgcagtac cagtggcgca
     7801 accacggcca tggttttaac catgatgcca taggcaacaa ctgcggccag cgcggatacg
     7861 ccatcgttat tggtaaagcc gagggcgaca ccgatcgcaa aaatcagtgg catgtttgca
     7921 aagacggaac cgcctgcttc tgccataaca tgcgatacaa cggcgggcag ccagctgaaa
     7981 ttcgcggaac cgacgcccag cagaatacct gcgataggca gtacggatac cggcagcatc
     8041 agcgatttac cgaccttttg caggttagca aatgcattct taaacataat tgagagtgct
     8101 cctgagtatg ggtgcttttt ttacgttctc acgcgtggca aggggggaga gcctcgccgt
     8161 gtacagggca tctaagcgcc ctttatttat tacacagagt aaaataattc agtgccaatt
     8221 tgtttgacgg ctatcacgtt tcaacttcag atcattggag aagttgcaca ttttgacaaa
     8281 acatatgtca caatatttca aacgtggtta ttcaccgccc aattttcggc ggtgaacttt
     8341 actcgtttta gccattaatt acgagcttta aaaaaactca ttcaacggat ggattgaagg
     8401 cgggaagagt cgatgtgaaa cagacgggcg aagttatcgg tggttacctg cgccagttct
     8461 tcaacggcaa cacctttcaa cacagccatg tattctgcaa cgtcacgaac catcgcaggt
     8521 tgattctctt ttcctcgatg cggtaccggc gcaaggtaag gtgagtcagt ttccaccagt
     8581 aaccgatcca gggggacata acgcgcagca tcgcgcagtt gctccgcatt acggaaggtc
     8641 acaatgccgg aaaaggagat gtaaaatccg agatccagta atttgcccgc cgtttctctg
     8701 tcctctgtaa aacagtgtag tacgccaccg caatccgtca ctttttcttc gcgcagaatt
     8761 gccagcgtat cggcgcgggc gtcacgggta tggacgataa ccggcttgtt cagttcacga
     8821 ccaatctgga tatgatggat gaaggactct tgctgacgaa ctttagtttc cggtgtgtaa
     8881 taataatcca gcccggtttc tcccagcgct acaacacctt cttctgccgc cagacggcgt
     8941 aaatcttcta catcgtaggg atcattctgg ttgagtggat gcacgccaca agaaaatacg
     9001 acgttgtcac gttcgcctac cagatcccgc atgtgtaaat agcccggtaa tgttgtggcg
     9061 actgccagac aaaaattcac atcgcgtgcg gcggctttcg ccagaacgtc atccacgtcc
     9121 ttatgcaaag attcataatc cagaccatcg agatggcagt gtgagtcgac taaaaacatg
     9181 atgtctctct taaagatgag gaaccggtag cacaacgccc ggttgcaggt aatgctcaat
     9241 acgcagtaaa agatcggtga tgagaagctc gcggttgatg cctgtgacag acattaactg
     9301 ttcacgaatg tggcaaacat cccccagtat agcctgcagg cgcgagggag aaagatggtt
     9361 tgccagttcg acgacaaggc ccggcacatc aacattggtc acctgcgcag caccatgatg
     9421 gcgttttagc gcatccatca gcaacgttgc cagccagtgt aaacgcgccg gagcttgttc
     9481 atgattaagg gccgccagca gcgaatacca gtcgcccgat ggcacgctat atgccaatgc
     9541 ctgacacaat gtttcacgag cctgccagtt atctccctga aacaacgcca gtgccgcgcc
     9601 aggcgaaccg gcgcttaagc gcaatgcggc aagtaatgca tcctgtgaca ttgtcacttc
     9661 gcgtgaaagc caggtcacgg cgtactgttc cggcggcggc gcaaggtaat gtagccgaca
     9721 acgactacgt aatgttgcca gtaaacgttc aggctcgcgg gtagccagga aaaaccaggt
     9781 ttctgctggt ggctcttcaa gcgttttcag caatgcgtta gccgcggcgt cggtcagtaa
     9841 ggcagcatcg gttacccaga caactttcgc accacctaag cgtgcgtgct cattcagctt
     9901 ttcggtgacc tcacgtaccg catcaatgcc caaagcattt ttcccttttt cgggagccag
     9961 ggtgtagtaa tcgggatgcg tgccagcctg catcaactga catccacgac agtgaccgca
    10021 acttttgtga ccctgcggtt gttggcagag taaataacgg ctcagggcgt agattaaagc
    10081 atcatcgccc atgcccggta acgcctgaat gagcagcgca tggtgacctc ttccggcctg
    10141 atagctggct accagttttt cgaaatcagg tcgtaaccat ggataccatc tcatgcgtcc
    10201 aactccttca cccagtgggt cacggtagtg cggattgcat ccatcacggc ctccagcggc
    10261 tgggtggcat caatggtatg aatgctttta tcttgtgctg ccagttccag atagcgggcg
    10321 cgggtgcgat taaagaaatc gaaagactct tgctcaatac gatccagctc gccgcgcgca
    10381 cgcgcgcgtt ttaagccaac ttccggggta acatcgagat agagcgttaa gtcggggcga
    10441 aaatccccga gaacagcatc acgcagtgtt gccagcatat gttggtcaat accacgtcct
    10501 ccgccctgat acgcctgagt ggagagatcg tggcgatcgc caatcaccca ggtgccgtta
    10561 gccagcgcag gtttgatgac tgtttctacc agttgaacgc gcgcggcata aaacatcaga
    10621 acttcggctt tatcggtaat gacttcatcg cctaccgatt tgatatccag caccaggctt
    10681 cttaactttt cggcaagttg cgtaccgcca ggttcccgag tgaaaaccat gtcgcggata
    10741 cccagttgct cgagcgtctc aaccaccaca ttacgcgcgg tagttttgcc tgcgccttcc
    10801 agcccctcaa tgacgatata cttactgcgc atttttttcc ttaagcactt tcagataatc
    10861 ctgcacagac ttgttatgac tggcaagatt ggtattaaac gtgtgaccac ctttaccatc
    10921 ggccacaaaa tagagatacg gcgtttttgc cggatgcgca gcagccttca gcgaatccgc
    10981 ccccggcgta gctatcgcac ccggcggcaa accggtaatg gtataggtgt tatacgctgt
    11041 cggcgtttcc aggtctgcac gagaaagttt gccattataa cgctctccca tcccgtaaat
    11101 cacggttggg tcggtctgca agcgcatacc aatgcgtaaa cggttgataa ataccgaggc
    11161 aacctgatcg cgttcactgg caacggcggt ttctttttcg ataattgatg ccatcgtcac
    11221 cagctgattt ttatctttat aaggcagacc gtccgcacgc ccttcccagg cgctatcgac
    11281 cgctttcacc attttcttgt gcgctcgctt gagtaacgcg acatcggtgg tattggcggt
    11341 atacatccag gtgtctggcc agaaccaacc ttcaatccac tccgggtttt ccagttcaag
    11401 tgcctgcgct acggtggcgt acttatcgtc gctcagcgta tgcttgatat acggggcctc
    11461 acgcaattgc ttgaggtaat cgctcagacg catcccttct accagtcgca gagggaactg
    11521 tgcttcttta ccgctttcca gcaacttcag catctcgcgc acggtcatct gcggtgtaaa
    11581 gcggtaagtc ccggctttaa agtgagaaag atccggttcg atacgcagca gccattgaaa
    11641 aacccgtggg cgattgatga tcttatcggc ataaagctgt tcaccgagcg ccagacgtcc
    11701 ggtccctggc ttcagggtaa atatcgtctc ttctttgata agcaatttgc tgtcggcaag
    11761 atggcgaacc ttccagacgc ccacaccagc ggcgataccc agtaccacca gcaataacaa
    11821 gataatcaat aacacttttt tcatgactaa ttcgggcgct cacaaagtgg ggctaaatat
    11881 tcatataacg ttgctgacga aaaggagaca tcgccacagg cacgtacggg catcactggc
    11941 attaacgcat tacaaataac catctcatct gcctgcaacg cctcttccag agaggcttgc
    12001 acttcgacaa gctgataaga ggattgtgcc agcaaacgga tacagaattg tcgcataatg
    12061 ccgttaacac ctgcctgatc cagtcgcggc gtataaacta cattgccctt ccgccagaac
    12121 aaattagccg cacagcattc cgtaacccac ccttcgctgt caaggaccag cgcctcatca
    12181 gcgtttgtct gctcaagatg agagcgaatc aatacttgct caagtcgatt aagatgttta
    12241 atacctgcaa gatgaggatt gcgccccagc cgcaccgggc ttagcgccaa cgtcatcccc
    12301 tcgttacgca aacggtcgta atgtgcagga taagccgtaa cggagagaat ccgcgttgcc
    12361 ggtccactgt tcaatgtgct gtaccctcgc ccgccactac cgcgactgat cacgactttc
    12421 agtacaccat tttgctgttc tgctgccagc gttttcatct cttgttcaag ctgaggccag
    12481 aagtcacagg aaatcatcaa ccgctgacaa gcatcatgta gtcgctggat atgcgcagat
    12541 aataaactga ctttaccgtc gataactctg gcggtggtaa aacaaccatc accaaactgc
    12601 gttgcccgat cgcttactgc cagcgattcc tgcttataac cgttaattaa gaacatacca
    12661 gctccttatc agagaattgt tagtgtggca gcatgttcac tacggaacaa gtcggaataa
    12721 aagctaagaa aaaaggcccg caagcggacc ttttataagg gtggaaaatg acaacttaga
    12781 tctttttaaa gatcaaagaa ccattagtgc caccgaagcc gaaggagtta cacagcgtgt
    12841 attccattcc gctgacctga cgtgcttcat gcggtacgaa atccagatcg caaccttcat
    12901 ccgggttatc caggttgatg gttggcggaa cagcctgatc gcgcagcgcc aggatggagt
    12961 agatagattc tactgcaccc gccgcaccta acaggtgacc ggtcatagat ttcgtggagc
    13021 ttaccaacac acggcttgca gcttcaccga agatggtttt caccgcctgc gcttcagctt
    13081 tatcgccagc cggcgtagaa gtaccgtgcg cgttaacgta gccaatctga ctcgcttcaa
    13141 tgcctgcatc acgcagagca tttgccattg ccagagctgc gcctgcgcca ttttctggcg
    13201 gtgacgtcat atgataagca tcgctgctca taccaaagcc gacgagttca gcgtaaattt
    13261 tcgcaccgcg ttttttcgcg tgttcgtact cttcaagtac cagcataccg gcaccatcgc
    13321 ccagtacgaa gccatcacgc tctttatccc acgggcggct cgccgcttgc gggttatcat
    13381 tgcgggtaga taatgcacgt gccgcgccaa aaccaccaac gcccagcggc gtactggctt
    13441 tctctgcgcc acctgcaacc atcacgtcag catcgccata cgcgataata cgcgcagcat
    13501 ggccaatgtt gtgcacgccg gaagtacagg cggtcgcgat agagatgctt gggccacgca
    13561 ggccatacat gatagtcaga tgacctgcca ccatgttcac aatcgttgac ggaacgaaga
    13621 atgggctgat cttacgtgga ccaccgttca tcagagatgt gtggttttct tcgatcagtc
    13681 cgaggccgcc aatcccggag ccaattgcgg caccaatgcg ggttgcgttc tcttccgtta
    13741 tttcaaggcc agaatcctgc atggcctgaa cgccagcgac aattccatat tgaatgaagg
    13801 catccatctt gcgctgttct ttgcgcgaga taatgtcctc acagttaaaa tcctttacta
    13861 agccagcaaa tttcgttgca taggcgctag tatcgaaatg gtcgattagg ctgatgccac
    13921 tctgaccggc aagcagagct ttccaggtag actctacggt attgccgaca ggagacaaca
    13981 tgcccagtcc ggtcacaact acacgacgct tagacacgtt tgtcctccag ggagggaaaa
    14041 aatgattcta gtgggacaaa aagataaaac tcaggcggtc gaacgaccgc ctggagatgt
    14101 tcacttacgc ctggtggccg ttgatgtaat caatggcagc ctgaacggtg gtgattttct
    14161 cagcttcttc gtccggaatc tcagtatcaa actcttcttc cagagccatt accagctcaa
    14221 cggtgtcaag agaatccgcg cccaggtctt caacgaaaga agcattgttg gtaacttctt
    14281 cctgcttaac gcccagctgt tcgccgataa ttttcttaac gcgttcttcg atagtgctca
    14341 tactcttaaa tttcctatca aaactcgctt tcgcgatggt tttcgtagtg tataaaatgt
    14401 tgaaaaattt gcaactaaat cccggcaggt cttaccacga ttttacgtta ttttgaggcc
    14461 ttacccccca ataacgcaaa tcattttgca ctaattatta ctcataacca cgcagactgc
    14521 gcgcaacatg agcattttgt gcaaatcgcg gtcagaccat gtacatcccg ccgttcacat
    14581 gcaaagtttc acccgtgatg taagatgctt cgtcggatgc caggaatgca accgcgttgg
    14641 cgatttcctg tgcgccgccg aggcgacccg caggaacctg cgccaggata cccgcacgct
    14701 ggtcatcgct cagcgcacgt gtcatgtccg tttcaataaa gcccggagca acaacgttta
    14761 cagtaatacc gcgtgacgca acttcgcgcg ccagtgattt actgaagccg atcaagcccg
    14821 ctttcgccgc agcgtagttg gcctgaccgc catttcccat ggtaccaacc acagaaccga
    14881 tagtgataat acgaccatga cgctttttca tcatagcgcg cattaccgct tttgacagac
    14941 ggaaaacaga tgaaaggttg gtttcgataa tatcgttcca ctcttcatct ttcattcgca
    15001 ttaacaggtt atcacgagtg ataccggcat tattgaccag gatatccact tcaccaaatt
    15061 ccgcgcgaat tttttccaga acagattcga tagatgccgg gtcggtcaca ttcaacatca
    15121 gacctttgcc gttagcacct aaataatcac tgatcgcctg agcgccattt tcactggtcg
    15181 cagtgccaat aactttcgcg ccacgggctg cgagcgtttc agcaattgcg cggccaattc
    15241 cgcggcttgc accggttacc agtgcgattt ttccttcaaa attcatgatt ttcctctttt
    15301 aaagctcgag cgccgctgcc atcgctgaag gttcgttcag cgccgaggcg gtcagggtgt
    15361 cgacaatgcg tttcgtcagg ccagtaagca ctttgcccgg gccgacttca tagagatgtt
    15421 ctacgccttg cgctgccatg tactcaacag acttcgtcca ctgaaccggg ttatacaact
    15481 gacgtaccag tgcgtcacgg atggcatcac cattggtttc gcatttcaca tcaacgttat
    15541 tcacaacagg aactgttggt gcgttaaagg tgattttcgc taattctact gccagtttgt
    15601 cggctgctgg tttcatcagc gcacagtgag acggtacgct cactggtaac ggcagagcgc
    15661 gttttgcgcc cgcagcttta caggcagcgc cagcacgctc aaccgcttct ttatgaccgg
    15721 caataaccac ctgtcccgga gagttaaagt ttaccggaga aacgacctga ccttctgcag
    15781 cttcttcaca cgctttcgca atagacgcat catccagacc gatgattgcc gccatagcgc
    15841 ccgtgccttc cggtacggct tcttgcatga acttgccgcg catctcaacc agacgcaccg
    15901 catcagcgaa atcaatcaca ccagcgcaaa ccagcgcgga gtattccccc aggctgtgac
    15961 cggccatcat tgccggtgct ttaccgccct gctgctgcca tacgcggtac agcgcaacag
    16021 atgcagtcaa cagcgctggc tgggtttgcc aggttttatt cagttcttca gctggcccct
    16081 gctgggtcag cgcccacagg tcgtagccca gcgccgcaga agcttcagca aacgtttctt
    16141 cgacaattgg atagctcgcc gccatatcag ccagcattcc aacggtttga gaaccctgtc
    16201 cagggaacac aaatgcaaat tgcgtcatgt tttaatcctt atcctagaaa cgaaccagcg
    16261 cggagcccca ggtgaatcca ccgccaaagg cttcaagcag aaccaactgc cccggcttaa
    16321 tgcgcccgtc gcgtacagct tcatccagcg cgcacgggac agaggccgca gaggtattac
    16381 cgtggcgatc cagcgtcacc acgacattgt ccatagacat accgagtttt ttcgccgttg
    16441 cactgataat acgcaggtta gcctgatgcg gaaccagcca gtccagttga gaacggtcaa
    16501 gattatttgc cgccagcgtc tcatcaacga tgtgcgccag ttctgttacc gcaaccttga
    16561 agacttcgtt gcccgccatc gtcagatgaa ttgaattctc tggattcaca cggtcagcat
    16621 taggcagcgt cagcaactca ccatagctac cgtcggcatg cagatgggtg gagatgattc
    16681 ccggctcttc agaggcagtc agcaccgcag cgcccgcgcc atcgccaaaa ataataatag
    16741 tcccacgatc ggttggatcg caggtgcgcg ccagtacatc ggaaccgacg accagagcat
    16801 acttcaccgc cccagatttc acgtattgat cggctacgct taatgcatag gtgaaacctg
    16861 cgcaggctgc tgcaacgtca aatgccgggc aacctttaat gcccagcatg ctttgaatct
    16921 gacaagctgc gctcgggaaa gcgtgcgtag cagaagtcgt tgccacaacg atcaggccaa
    16981 tctggtcttt ctcaatgccc gccatctcaa ttgcgcgtgt cgccgcttca aagcccatgg
    17041 ttgaaacggt ttcgtttggc gcggcaatgt ggcgttcgcg gataccggta cgagtgacaa
    17101 tccactcgtc agaggtgtcc accatttttt ccaaatcggc gtttgtccgc acttgttcgg
    17161 gcagatagct gccagtacca ataatcttcg tatacatgta cgctcagtca cttttcggtt
    17221 atataccgtc acttgcaaac tgcgagttcg ctggcagcgt cctgctaccg cagagttccg
    17281 cttttgccac cgtccagcag ctcaaaacca gctgggtata cagattccag gcgagcggca
    17341 attcgctgag gaacttgtcg ctgcaccgcc tgcactgcct gttcaatcgc gaccgcaaaa
    17401 gctcgctgat tggctgcacc atgacttttt atcaccgtgc cgcgcaatcc taacagacag
    17461 gcgccgttat actggtcggg gttgaggtga ctgaatcgcc tcgtcaggct cttttgtagc
    17521 caacgcttta ataacagtag ccaccacgac cgttttttcc cttcaccctg agatttcagc
    17581 agagaaagga acatcctgac aacaccctcc atcgtcttta atgtgacatt tcctgtaaag
    17641 ccgtcacaaa ccagcacatc tgtcttgcca gttaacaact cattggcttc aagatagccg
    17701 atataattga tagaagggat tgttttaagc accgctgagg catcccgaat actgtcgaga
    17761 ccctttactt cttcttcacc aatattgagc aacgccacgc gaggattggg aatttccacc
    17821 acctcttcag ccagaactga gcccataatg gcaaattgca ccaacattgt actgtcacaa
    17881 tcgacgttgg cccctaagtc aaggaccacc gttttgccct tttgctgatg tggtaatacc
    17941 gtcaccagcg ccggacgctc aatcccctcc aggggcttga gtaataattt tgccagcccc
    18001 atcagtgccc cggtattacc ggcactgaca caggcttgcg ctcgaccttc tttcaccagc
    18061 tccagggcca cgcgcattga actcccacga ctggcgcgga tagcttgcga aggccgggca
    18121 tcactggcga taactgactg cgcaggaata atctgcagac gcgaacgttg ttcaaagtca
    18181 gctttagcaa gtaatggcgt gatggcgtcg ggattgccga ctaaaagaag agtgagttgc
    18241 gaattagaat tcagtgcctg caatgctgca ggcactgtca cggaagggcc aaaatcccct
    18301 cccatgacat ctaacgccag ggttagacgt gtcaaggtat cgtcgccgcc cggtttggtc
    18361 tttccccagt tgcctgggga aaatcctcac taagcttcat cacgcagacg cgtgattact
    18421 tagcgatgac cttgcggccg cggtagtaac cgtcggcagt gatgtggtga cgcaggtgtt
    18481 tttcaccaga agttttgtct acagacaggc tggtgactgc ggtcagcgcg tcatgggaac
    18541 gacgcatgcc acgtttggaa cgggttggtt tattctgttg tacggccatg gaccttactc
    18601 ctcaattacg gtttatcccc gatccaacgg ggagcaccaa ttacttacgc tttaagctgg
    18661 ctaatacggc aaatgggttt ggcttttgcg cttcttcagg cagttcacca aagaccatgt
    18721 ccgcttcgga cacttcacag tgttcagaat catgcaccgg aactaccggc aaggcgagga
    18781 tgatttcatc ttcaaccatt gcaagcagat cgatttcacc gaattcgtta acctcaatcg
    18841 gttcatacgc ttccggcagt gcttcagcct gttcgtctga acgcacagga ctaaaacaat
    18901 acgttgtgta gacctgatga gtaaacggct tcccgcaacg ctgacactcg agcgttaccg
    18961 tcaccttcgc atcgccgttt aacactgcga gacgttggtt atcgatagcg aacgacatgg
    19021 agcattccac atcactgtcc acactgacta cggattcggc gacgcgctca acctgaacag
    19081 gggtatagat accctggtaa tcaaggcgtt tttgagccgt acgaaccgga tcgagagtca
    19141 ggggtaattt taccttttgc atagggcgcg catattaact ttgtaacgtc atagagtcaa
    19201 agaaaaaggc agcctgtggt tgccttttgc caataattcg cacacattgc gggttactgc
    19261 tttattttcg ttcagtgctg ataacgaaca tcaacctgca aaacgaagcg catagtttaa
    19321 aatggctatc atcaatacgc tatatctgat ggaaaaaaat atgcctaaac ttattttagc
    19381 ctccacctcg ccatggcgtc gcgctcttct ggaaaaactg caaatctctt ttgaatgtgc
    19441 agcaccagag gtcgacgaaa ccccacgcag cgacgaatca ccgcgacagt tggtgcttcg
    19501 actggcacaa gaaaaagcgc aatctctggc gtcacgttat ccggatcatt taattattgg
    19561 ttcagaccag gtatgtgttc ttgatggcga aatcaccggt aaaccgttaa cggaagaaaa
    19621 tgcccgtctg caattacgca aagccagcgg caatatcgtc accctctata ctggactggc
    19681 gttgtttaat tcggcgaatg ggcatctgca aacagaagtg gagccttttg acgtccattt
    19741 ccgtcacctg agcgaggcgg agattgataa ttacgtgcgt aaagagcatc ccctgcactg
    19801 cgcgggtagc tttaagagtg aaggatttgg cattacgctg tttgagcgtt tagaggggcg
    19861 tgatcctaac acgctggttg gtttaccgct aatcgcgctg tgtcagatgt tacgtcggga
    19921 agggaaaaac ccgctgatgg gatagttttt tgcgccggat gtggttacgt cgcatccggc
    19981 aatcggtgct ggcggcctga tgcggcgtaa acgccttatc aggccaaccg cccattatca
    20041 ggtttatatg cttgtattag cgcgcgttac gcagcttttg caaacaacgc ttcaaacctt
    20101 catccatcgg agcttcgata cgcatcacct caccggtccc cggatgagta aacttcaacg
    20161 ctgcagcgtg caggaacagg cgatttaatc ccgtgcctgc ttcagtgagc tgcctgtcaa
    20221 attcacggtc accgtagcga tcgtcaaagg caatcggatg acccgcatat tgtgtatgca
    20281 cacggatctg gtgagtacgc cctgttaccg ggctacaacg caccagggtg gcaaatgcat
    20341 agcgttcttc cactttaaag cgtgtttccg acggtttgcc ttcctgactc acacgcacga
    20401 tacgttcgcc gctttgcaga atatttttca gtaacggtgc ttgaacgctc ttcacatgcg
    20461 actgccactg accgcgcacc agcgccaggt aatctttttg catccctttt tcacgtaatt
    20521 gctcatgcag agaacgcaac gccgagcgtt ttttcgctac cagcaaaaca cctgaggtgt
    20581 cccggtcaag acgatgaacc agttcaagga accgcgcttc cgggcgcaac gcccgcaaac
    20641 cttcaataac gccgaagctt aaaccactgc cgccatgtac cgccgtaccg gaaggtttat
    20701 tcagcaccag gatgtgatca tcttcatata agatgacgtc cgccagcgcc gccacctttt
    20761 gcagatgtgg cgaaaccgcc tcttcttccc gctcagcaac gcgaaccggt ggaatacgca
    20821 cctcatcacc cgcttcgagt ttatattcag gcttaatacg ttttttgttc acccgcactt
    20881 cgcctttacg caaaatacgg taaatcatac tttttggtac gcctttcaat tgggtacgca
    20941 aaaagttatc gatacgttgc cccgcttcgt cagcggtgat agcaacaatt tttacggatg
    21001 gagtctctgt tttcatggtt ggcgattcta attagccaac aggattcgcg ccactcattt
    21061 ttctatgctt atatttactt ttgcacctta ttacttcact gcgtgatcac tttattgatg
    21121 gttattaaac caatcaccag caagaagtga aaaaactgtg agtaagcggg tgataaatgg
    21181 taaaagtcat cttgctataa caaggcttgc agtggaataa tgaggccgtt tccgtgtcca
    21241 tccttgttaa aacaagaaat tttacggaat aacccatttt gcccgaccga tcatccacgc
    21301 agcaatggcg taagacgtat tgatctttca ggcagttagc gggctgcggg ttgcagtcct
    21361 taccggtaga tggaaatatt tctggagagt aatacccagt ctgtttcttt gataattgcg
    21421 ctgtttttcc gcatgaaaaa cgggcaaccg acactctgcg cctctttgag ctgacgataa
    21481 ccgtgaggtt ggcgacgcga ctagacacga ggccatcggt tcacacccgg aaaggcgtta
    21541 ctttgcccgc agcttagtcg tcaatgtaag aataatgagt aagttacgat gaaaagaatg
    21601 ttaatcaacg caactcagca ggaagagttg cgcgttgccc ttgtagatgg gcagcgtctg
    21661 tatgacctgg atatcgaaag tccagggcac gagcagaaaa aggcaaacat ctacaaaggt
    21721 aaaatcaccc gcattgaacc gagtctggaa gctgcttttg ttgattacgg cgctgaacgt
    21781 cacggtttcc tcccactaaa agaaattgcc cgcgaatatt tccctgctaa ctacagtgct
    21841 catggtcgtc ccaacattaa agatgtgttg cgtgaaggtc aggaagtcat tgttcagatc
    21901 gataaagaag agcgcggcaa caaaggcgcg gcattaacca cctttatcag tctggcgggt
    21961 agctatctgg ttctgatgcc gaacaacccg cgcgcgggtg gcatttctcg ccgtatcgaa
    22021 ggcgacgacc gtaccgaatt aaaagaagca ctggcgagcc ttgaactgcc ggaaggcatg
    22081 ggacttatcg tgcgcaccgc tggcgtcggc aaatctgctg aggcgctgca atgggattta
    22141 agcttccgtc tgaaacactg ggaagccatc aaaaaagccg ctgaaagccg cccggctccg
    22201 ttcctgattc atcaggagag caacgtaatc gttcgcgcat tccgcgatta cttacgtcag
    22261 gacatcggcg aaatccttat cgataacccg aaagtgctcg aactggcacg tcagcatatc
    22321 gctgcattag gtcgcccgga tttcagcagc aaaatcaaac tgtacaccgg cgagatcccg
    22381 ctgttcagcc actaccagat cgagtcgcag atcgaatccg ccttccagcg tgaagtccgt
    22441 ctgccgtccg gtggttccat tgttatcgac agcaccgaag cgttaaccgc catcgacatc
    22501 aactccgcac gcgcgacccg cggcggcgat atcgaagaaa ctgcgtttaa caccaacctc
    22561 gaagctgccg atgagattgc tcgtcagctg cgcctgcgtg acctcggcgg cctgattgtt
    22621 atcgacttca tcgacatgac gcctgtacgc caccagcgtg cggtagaaaa ccgtctgcgt
    22681 gaagcggtgc gtcaggaccg tgcgcgtatt caaatcagcc atatttctcg ctttggcctg
    22741 ctggaaatgt cccgtcagcg cctgagccca tcactgggtg aatccagtca tcacgtttgt
    22801 ccgcgttgtt ctggtactgg caccgtgcgt gacaacgaat cgctgtcgct ctctattctg
    22861 cgtctgatcg aagaagaagc gctgaaagag aacacccagg aagttcacgc cattgttcct
    22921 gtgccaatcg cttcttacct gctgaatgaa aaacgttctg cggtaaatgc cattgaaact
    22981 cgtcaggacg gtgtgcgctg tgtaattgtg ccaaacgatc agatggaaac cccgcactac
    23041 cacgtgctgc gcgtgcgtaa aggggaagaa accccaacct taagctacat gctgccgaag
    23101 ctgcatgaag aagcgatggc gctgccgtct gaagaagagt tcgctgaacg taagcgtccg
    23161 gaacaacctg cgctggcaac ctttgccatg ccggatgtgc cgccagcgcc aacgccagct
    23221 gaacctgccg cgcctgttgt agccccagca ccgaaagctg caccggcaac accagcaact
    23281 cctgcacaac ctgggctgtt gagccgcttc ttcggcgcac tgaaagcgct gttcagcggt
    23341 agtgaagaaa ccaaaccgac cgagcaacca gcaccgaaag cagaagcgaa accggaacgt
    23401 caacaggatc gtcgcaagcc tcgtcagaac aaccgccgtg accgtaatga gcgccgcgac
    23461 acccgtagtg aacgtactga aggcagcgat aatcgcgaag aaaaccgtcg taatcgtcgc
    23521 caggcacagc agcagactgc cgagacgcgt gagagccgtc agcaagctga ggtaacggaa
    23581 aaagcacgta ccaccgacga gcagcaagcg ccgcgtcgtg aacgtagccg ccgccgtaac
    23641 gatgataaac gtcaggcgca acaagaagcg aaggcgctga atgttgaaga gcaatctgtt
    23701 caggaaaccg aacaggaaga acgtgtacgt ccggttcagc cgcgtcgtaa acagcgtcag
    23761 ctcaatcaga aagtgcgtta cgagcaaagc gtagccgaag aagcggtagt cgcaccggtg
    23821 gttgaagaaa ctgccgctgc cgaaccaatt gttcaggaag ctccagctcc acgcacagaa
    23881 ctggtgaaag tcccgctgcc agtcgtagcg caaactgcac cagaacagca agaagagaac
    23941 aatgctgata accgtgacaa cggtggcatg ccgcgtcgtt ctcgccgctc gcctcgtcac
    24001 ctgcgcgtga gtggtcagcg tcgtcgtcgc tatcgtgacg agcgttatcc aacccagtcg
    24061 ccaatgccgt tgaccgtagc gtgcgcgtct ccggaactgg cctctggcaa agtctggatc
    24121 cgctatccaa ttgtacgtcc gcaagatgta caggttgaag agcagcgcga acaggaagaa
    24181 gtacaagtgc agccgatggt gactgaggtc cctgtcgccg ccgctgtcga acctgttgtt
    24241 agcgcgccag ttgttgaaga aatggctgaa gtcgtagaag cccccgtacc ggttgccgaa
    24301 ccgcaaccgg aagtggttga aacgacgcat cctgaagtga ttgccgccgc ggtaactgaa
    24361 cagccgcagg tgattaccga gtctgatgtt gccgtagccc agaaagtcgc agaacacgca
    24421 gaaccggtgg ttgaaccgca ggaagagacg gcagatattg aagaagttgc cgaaactgct
    24481 gaggttgtgg ttgctgaacc tgaagttgtt gctcaacctg ccgcgccagt cgtcgctgaa
    24541 gtcgcagcag aagttgaaac ggtagccgcg gttgaaccag agatcaccgt tgagcataac
    24601 cacgctaccg cgccaatgac gcgcgctccg gcaccggaat atgttccgga ggcaccgcgt
    24661 cacagtgact ggcagcgccc tacttttgcc ttcgaaggta aaggtgccgc aggtggtcat
    24721 acggcaacgc atcatgcctc tgccgctcct gcgcgtccgc aacctgttga gtaataatta
    24781 gctcaaagta atcaagccct ggtaactgcc agggcttttt tatttcatgt ttgaatcaac
    24841 atattaaaat tcatcaaatg gcatccttgc taaccaacaa tgcaaaatag gcaaaaaaaa
    24901 tgcccgcccc ggcgatccca gagcgggcag acaaaacata cccagtttca tgatatgttt
    24961 taaagcgagc ttatttgttg agctggaaga gcgacaatcc ctgcatatcg gtaaatgctt
    25021 tatacgatgc ctgcaatgcc gtttgctgca tgatgtaaga tgaaatagtt gcattccagt
    25081 ccacatcaac cagatcgctc atctgctgcg tttgccctaa agcgcgatcg ctacctaagg
    25141 aatccagcga ctccagttcg ttcagctgcg tgcctaattc cgcgcgaacg gtaaggacat
    25201 tattcagcga gtttttcagt ccacggttgg ttttatccaa tgccgcagcg gcgatttctt
    25261 tatccgcttc gctatccgcg accggcgttt tcagggctgc gatggcgcta tccagcatgg
    25321 caaaaagatt ggtttcagaa gcgctaccgt ctggttccgc taccgcgtta ctggtaatac
    25381 tgtcgaaaat tttgtcgccc gtatgtccta tcaccatcga acgcgaagca tcgacctgtt
    25441 gcttaatact ttctgttccg ccgacataat caccgttagc ctcactaaac ggcgcagtct
    25501 ctgttttata accggcaaaa atgtagcgcc cattaccgtc agtggtgttc gccagattca
    25561 gcaactggtc acgaaacccc tgaatatccg tagccagcga ggcccggtca tcgtcactca
    25621 aggtgccatt gctggcgtag acaatttttt cctgagcatt ctggatagca gtggtgacct
    25681 ggctaagtac actctcttcc agtgacactt tttgagtggc gaaagtacgt gccagcgtgt
    25741 actggctgtt ttgcgcctgt gcctgggaga gaacaacagc ttgtgatgca gcaatgggat
    25801 cgtctgaagg gttaacgact cgcttacccg tcgacatctg ctcgccgtac ttcatccatt
    25861 ctgcctgaga attggtgatg ccgcgcatgt tttgctggta catcatctgg gtactgaaac
    25921 gcattttatc tccccttagc gaatgttaat cagcgcatca aaaatcgcgt ttgccgtctg
    25981 cagaacctgc gcattcgcca ggtaatactg ctgaaaacgt tgcagatttc cgtactcctc
    26041 atcgagattg acaccggaaa tcgactgctg ctgattggaa agctgcgtca ccacattacc
    26101 ttgcgtggcg ctactggttt tcaacgtcgc ggttttatta ccgatatcac tcactaacga
    26161 ggcataagcg tcgttaaagg atttcgcacc gcccaccgtt ttactgttgc tttgcagatc
    26221 cagcagggcc tgaccgttgc ggttatcgct atcacccgca tcttcttcgc tcgccatcgc
    26281 tattttcgct tcgtcggtga ttaatacatc catgttgacg atagcgtcac ttactggttt
    26341 cagcgtgaag ctgtcgttaa cagcaggcgt tcctgtaaac gtcaactcca gaccatcaaa
    26401 tgccacttta ccgttggcat ccggcgtcac cgtaaaagtg gtattactgg caaggcgggt
    26461 gacctgccac tgattattat cgaacgagat tttgtaatca gtcgccagta ccgcggaggc
    26521 atcagttacc gtggcaccga tcgcaacgtc acctgtgttt ttcgtgtttt gcagaaccgc
    26581 gggcttaccg atagcaaaga aatcttcacc agcatcgccg ttggcatcaa atccggcttt
    26641 gtgttggctg ttgaaagcct cggcaaatgc cagcgccagt tgtccaagcg tattacgcgt
    26701 ctggtccaga tcctgagaac ggaatgtcag aatgccgccc agcgacccgg tattcagtaa
    26761 tttctccggg atctcaatat tgcctgccgt cccatcaacg taagcgacag tcgtacgaga
    26821 agggtcggcg ctggaaggaa ctgccgccag ttgccgcgcc gtacttccct gaaccagtga
    26881 gtaaccattg gccatcgtga tgttataagt accgccatcc tgaacgctga cttctacgcc
    26941 aacaatctgg tttaattcgc tcaccagttg atcgcgttga tccagcagat tgttaggtga
    27001 cgcccctgcc cccacgcctg tcaggcgcga gatttgatcg ttcaggctgg caatttgttt
    27061 agcgtaattg ttgatctgat caacgctggc acctatcgcg atattgacct gtttgtcctg
    27121 gtcgcgcaga tattgatcgg tggttttaaa ctgattcacc aacccttctg atttcccaat
    27181 cagcgcctgg cgcgctgccg ggtcttccgc gttactcacc agcgtttgca ggctggtgaa
    27241 gaaatcctgc atctgtgttg ccagcgaaga ggtactggtg gagagcatat tgtcgatttt
    27301 cgacatctgc tcatagcggg cagtcaggcc gctactttgc gtctgcgccg cacgtaactg
    27361 gttggtaata aaagcatcat actcacgctg cacaccagaa acgtagacac cattgccaac
    27421 ccagccgcca gcgcccaacg tgctattggc ctgcgccata atagtggttt ggcgggtata
    27481 tccggcaacg ttatagctgg agatattatt acttgccgta tttaacgccg cctgggccgc
    27541 gttcagtccg ctcatggcgt tattgatcaa gctggacatg gaggttcctt ataagccttc
    27601 aattacagag tattatcggc agcgacccgc cggacttgag ttattcagaa cagattatca
    27661 atgttcatgc tgtaggtttt gctcaccttg tcgcttatcg atttcatctg ctggatcatg
    27721 ttggtgagtt tacgggcata gtgaggatcg gtagcatacc ccgcgtcctg tagggcctgc
    27781 gccccctgct ccgcactcgc ggcggtcgtc acggcggcgt agcgcgggtt acgtgttaac
    27841 aacccaacgt aatccgacaa tgcttccaga tacgagctgt agacccgaaa tttcgctttt
    27901 actttcttcg cttcgccatt ttcatattca gtcgtggtga tttcagtgac tggccctttc
    27961 cagttgccag aggctttgac accaaacagg ttatagctcg gctcgccgtt ttcgcggcgg
    28021 atttgtcgtt gcccccagcc agattccagc gccgcctgag cgaggatcaa atgatgtggc
    28081 acaccgcttt gctggcttgc cagttgggcg ggcagcgaga gttgcgcgag gaatgcttta
    28141 ctgtcacccg gcagcgaatc atcgtagtta cgtggtacgg ccttttgcac cagctgagaa
    28201 agcgcctgat tttgataacg caccacagtt tcgagcggga atttcatcgg tgctgctggc
    28261 gtgggctcct ctggcaatgg ttgttctggc gtcatctgtt taaccatcat ctctgcaagc
    28321 cccagacctt tgcccgccgt catctgttgg gcaatctgct ggtcatacat actggtatac
    28381 aggcgagtgt gctcgctgct gaacaggcca tcttttggta aagcgtcgcg catgcttttc
    28441 aacatcatct gcacgaacat cccttccacc tggcgggcca ccggacggat atttgccgcc
    28501 ggatcttcgc ccgctttcgc ctttagttcg ttgagtgatt gcgcatccca ggccgcactt
    28561 gccagtagtt tgctgtcgct gatcatcaga tgatttccag ttttgcccgc agacatcccg
    28621 cactttgcat tgattgcaaa atagacatca gatccatcgg cgtagcgccc agcgcattga
    28681 gcgcgcgcac cacgttattg aggctggcgc tggaacgtac gctttgcagc gaaccgccgc
    28741 tctggcgtaa gtcgatctgc gtttgtggcg ttaccacggt ctgtccgcca ccaaacggtg
    28801 tatctggctg gctgacattg gcctgacgat tgactgtaac tgagagattc ccctgcgcta
    28861 ccgcgcagct gtcgagggtc acttcgcgat tcatcaccac cgaaccggtg cgcgagttaa
    28921 tcactacttt agcgtcctgc ggggtgacat taacctgcat attctggata tcggcaagga
    28981 aacggacctg ggaactgttg ccacttggta cgcgcacctg aatagtccgc gcatctaacg
    29041 cggtggcgct gccatatcca cgcacgcggt tgatggtgtc agcgatttgc tgcgccatgc
    29101 tgaaatcttc gtcgttaagt tgcaaattaa gggtattccc gacgccaaac tggctgggca
    29161 attcacgttc aataaccgca ccattggtga tccgtccacc gttcagttgg ttcacctgaa
    29221 cactgctacc gccagcggag gctcctgcgc cgccaaccag aatattgccc tgcgccagcg
    29281 catacacctg actgtcaacg cccttaagcg gtgtcatcaa caacgtacct ccacgcaagc
    29341 ttttggcatt acccatggag gaaaccacca catcgatggt ttgcccctga cgtccaaacg
    29401 gaggaagtga cgctgtcacc attaccgcag cgacgttttt tagctgcata ttggtgcccg
    29461 tcggaacggt aattcccagc tgtgagagca tgttattaag cgtttgtgtg gtaaacggcg
    29521 tctgggtcgt ctggtcaccg gttccatcca gccccaccac caggccatag ccaatcagtg
    29581 agttttgcct taccccctga acactggtga gatcgcgaat acgctcagcc tgagccgccg
    29641 tcgtaaccag tagaagaatt aatgcagaga gaaatttaat caccacagcc tcacttacat
    29701 tggcgacagg ttaaggaaga aacgttgcaa ccagcccata ttttgcgctt cgttaatgta
    29761 gccattgcct acgtattcaa tgcgcgcatc cgccacctga gtagacggta cggtattgct
    29821 gccgctgatg gtgcgtggat taaccacgcc agagaagcga ataaattcgg taccctgatt
    29881 aatggcaatc tgtttttcac ccaccacatg caggttgccg ttgaccagta cctggtcaac
    29941 cgtcaccgtc aacgtgccgc taaaggtatt gctggcattg gccccgcctt ttccgttgaa
    30001 cgtgttacca ccggaggctt cgacatcggc acgagcgtta ccaaacagcc cctgcaaata
    30061 gcgcggcaca gtatcaaagc caaaattagt tttaccgtca cggctggcat tcgcagagga
    30121 gcttttgctg gcgctgacgt tctcctgcaa cacgatggtc agcgtatcgc caatattgcg
    30181 tggtcgacga tcttcaaaca gcggttgata gccatagtta atcggctgag cagactggaa
    30241 aatagaaccg ttggcgacgg gagtcggacc gggaaccggt tgtgcactgg tcgctccctg
    30301 taccagcggc gtggagggta tccaggcgca gccggttagt gaaagcacca acaagctgga
    30361 aatggcataa gtatgcgcag cgtttttttg catggctatc atcttcaaaa atcaataaac
    30421 cggtgaacct gccaccggtt atgccttaga gttgcgtcag tttttgcagc atctgatcgg
    30481 tggtggacac cgctttactg ttgatttcgt aagcgcgttg cacctgaatc atattgacca
    30541 gttcttccgc cacgttgacg ttagacgttt caacataccc ttgatacagc agtcccgcac
    30601 cgttcaggcc cggcgtgctt tcgttcggtg caccggagga ttgcgtttcg gtgtagaggt
    30661 tttcgccaat gctctccagt ccggtatcat tcataaaggt ggtgagattg agctgcccaa
    30721 cctggaccgg agctgcctgg ccttgttggg ttacgctgac cacgccatca cgaccgatgg
    30781 tgatacttaa cgcattcgcc ggaatggtga tcgctggctg cacctgaaaa ccaccagccg
    30841 tcaccaactg cccgttctga tccacctgga aagagccgtc acgggtatag gctgatgagc
    30901 catctggcaa catcacttgg aaaaagccct gccctttaat cgcgacatct ttgctgttgt
    30961 tggtctgcga caggttgccc tggctgtgta agcgttcagt ggcgaccggg cgtacccccg
    31021 tgccgatttg taatccggag ggtaaggtgg tttgttcgga agactgtgcc cccggctgac
    31081 gaatggtttg ataaagcaga tcttcaaaca ccgcgcgctg acgcttaaaa ccgttagtac
    31141 tgacgtttgc caggttgttg gcaatgacgt ccatattggt ttgctgggcg tcaaggcccg
    31201 ttttggcgat ccataatgaa ctgatcataa aatgtcctgt agtggattag tgacaaagtg
    31261 catatgtctt cttgccggat gcggcgtaaa cgccttatcc ggcctacaag tgctcgcaaa
    31321 atcgataaat tgcgattgcc cccatagacc cgataaactt gcgcatcggg caacttatac
    31381 ttgtcatgta tcctttcaat taactcatcg acagcagttg gttggcacgg cctgcgttat
    31441 catcgacgct gctgatcacc ttcatctgca tttcaaaacg ccgcgcgctg gcaatcatgt
    31501 cgctcattgc cgcaacggca ttgacgttac tgccttccag aacacccgac atcacacgca
    31561 aggttggatc tgcctgcagt acaggcccac gcgtggcctg ggtttctgcg cttaaacgaa
    31621 aaatgccgtc gtcaccgcgc tgcacttcgc tgcccgtggc tttcaccagc ttcagacgcc
    31681 ccactggcgc aaccgtattt gccggatcgc ccggattcag cgccgagatt gtgccatcgg
    31741 cagctatcgt gatttccgcc ccttccggca cagcaattgg ccccgcctcg cctatcaccg
    31801 gatgcccctg aattgtcagt tgcccggtgg gatcaacctg aatgctgcca ttacgcgtat
    31861 acccttcgct gccgtcagcg gtctgcacgg ccagccaacc atcctgctgc aacgcaacgt
    31921 ccagcgggcg cgaggtgtaa tccatcttgc cgggcgtcat gtctgcaccc ggtgttgacg
    31981 ccgttaccag cgtgcgcgta ggcagtgaaa gcccttccac cggcactgcg cgcaaggcgt
    32041 ttaactgcgc acgaaaaccg ggcgtagagg cattggccag attactggcg gttaccgcct
    32101 gttgattcag cgtctggctg gctgctccca tcgcggtata aattgcgtga tccattgagc
    32161 tatcccgtca gcgattagcg taagttaacc agcgtgttga ggatctggtc ctgggtttta
    32221 atggtctggg cgttagactg atagttacgc tgggcaacga tcatattgac cagttcttta
    32281 ctgagatcga cgttggacgc ttccagcgca ccgttggtca gggtaccaaa gttgcccgtc
    32341 ccggctgtcc ccaacagcgc cacgccagaa gattgcgtcg cagaccagac gttgtcgcct
    32401 tcggatgcca gaccttcgtt gttggcaaag ttcgccagta caatctgccc cagcagttgg
    32461 gtttgttcgt tggaatagtt gccgacaacc gtaccgtcat cattgatttg ataactcacc
    32521 agatcgcccg gtttgtagcc gttctgggtg gttgccacaa tgttgttagc gccggtattt
    32581 tgctgcatgg agttgaggaa gctcaggcta aacgtggcgg gatctgcgcc gttaattgcg
    32641 ccagtggtaa tattctctgt tggatttgag gtcagaacgc cattggcatt aaacaccagc
    32701 ttcattgcag gctcggctgt acctgttgga tcactgctat cctgggtgta gacctgccag
    32761 ttattatccc cggtcttcac aaagtagacg ctcatgtcat gagcattacc ctgactgtcg
    32821 aaaacagtca ccgaaccttt tttgttatag ctatccgcat tgctggcgct gaatggcgta
    32881 acagtaggaa gcggatcact ggaattcagg ttgatctgca tcgacgccgt ggtggtagtt
    32941 ttcgctgcca tcagggtatt cgggatcgaa atgttagtcg gattcgcccc ttgctgaata
    33001 gtcggcggcg taccggttgc cgggtaaccg gtcagctgta aaccttgcat attcaccagg
    33061 ttacggtttt catccagctt aaattgtccg ttacggctgt agaacaccga accgttgctg
    33121 tctaccagac ggaaaaaacc gttctggctg atagcaacgt ccagaccacg cccggtgttg
    33181 gtggtcgtgc catcggtaaa gtcctgagtg ataccggcaa cttttacccc cagtcccact
    33241 ttcgaaccgg caaacatatc ggcaaaagag gccgtgcctg atttaaagcc gtatgtggcg
    33301 gagttggcga tattgttgcc aataacatcg aggttggtgg cggcagcgtt taatccgcta
    33361 accgcttgag aaaaggccat gactgactcc tgaaatgtga aggcttaaat tatctgccgt
    33421 acttcgtcga gggtggtggt gccgtaagtg ccgagatcca gcgtattacc gttgttgccg
    33481 cggatcacac cctgcaccag agcaaactgc agcggctggg caaccagttg tgtaccaccg
    33541 ttactggcgc taatcgctac gttgtaagaa ccgttcggcg cagtgctgcc gtcagtcaac
    33601 gtaccgtccc aggtgaaact gtggactccg gcggtcagtt caccaatatc aatggtgcgc
    33661 acaaccgcgc catttttatc ggtgatggtg gccgtaactt tgtctgccgc ctgttgcagc
    33721 tcaacaccaa acggcgtggt cgtggtcaca gccccttctt cactgccggt tcccgcaaga
    33781 acagtggtgc cggggatcat cacgccgtga ccaatcaggt tactggcctg taacgactgg
    33841 ctgttatcaa tctgtccgga aatagatccg agcgtggtat tgagtttttc aatcccgctg
    33901 accgtgctga tttgtgccaa ttgcgacgtc agctcgttgt tttccattgg attggtcggg
    33961 tcctggtttt tcagctgcgc caccagcaaa gtcagaaaac tgctttgtaa atctgcggcg
    34021 ttgctgcccg tgagcgaact actgctggtg gtactgacgc cggtatttgt cggatcggtg
    34081 gtggttaccg caatggacat agctttctcc tttattgacc gagcgtaagg gttttcagca
    34141 tcatgctttt caccgtgttg agcacttcaa cattggcctg atagctgcgt gacgccgaca
    34201 tggtgttaac catctccccg acgacatcaa cgtttggcat tttcacgtag ccctttgcat
    34261 ccgccagcgg attacccggt tcataaacca gtttgtccgg ggcctgactt tctataacat
    34321 cggcaacctt tacgccgcct gtcgcagcac ctggtgcagc gttaacctgg aataccacct
    34381 gttttgcccg atatggctgt ccatcaggac cggtcacgct atcagcattc gccagattac
    34441 tggccgccac gttcaggcgc tgggactggg cagttaacgc cgacccggcg atatcaaaaa
    34501 tattcagcag tgccatccgt taatttccgc tctgtaaaac gttcatcatg cctttgattt
    34561 gcccgctcaa cgcgctaagg ctcatctggt attgcaggct gttatcggca aactgggtgc
    34621 gttcgcgatc catatcgacg gtattaccgt caagcgaagg ctggtccgga atacggtatt
    34681 gcagttctgc ggtaggaggc gtcagcgcct gcgccggaat gtgttgcgtt gaggtcatcg
    34741 tcagtgcaac cacactggtt gcatcccgtc cacgttgcat gactttttta agttcactgg
    34801 caaaatcgat atcgcgcgcc tgataaccag gggtatcagc attggcgatg tttgctgcca
    34861 gcacttcctg acgctgtgcg cgcagattga gcgcctcttg ttgaaaacgt aaggcggcgt
    34921 cgagcttatc gagcatatct cctccgcagg tatcaaaatt ctgccatcag cttaaatgcc
    34981 tttacgcgcg cgttatcggc ggaataaacg caaaatgggt cgctatttat gccgttgatg
    35041 accattgcgg acaggtacaa ttcacgttgt agaaatggct gggggcgaaa atgctggcaa
    35101 taaaacgtag cgtggcgatc atcgcgatac tgttcagtcc gttaagtgcg gcgagtaatc
    35161 tcacatcgca attgcaaacc ttttttagcg cccagctcgc agggataagt gatgaggttc
    35221 gtgtttctat tcgtacagcg cccagtctac tgccgccgtg cgagcagcca ttgctttcga
    35281 tgagcaataa ttcccgcctg tggggcaatg tgaatgtgtt ggctcgctgc ggtaacgaca
    35341 aacgatattt acaggttaat gtacaggcca caggaaatta tgtggttgcc gcgatgccca
    35401 ttgtgcgggg aggaaagctg gaagctggca atgtcaaact gaaacgcgga cggctggata
    35461 ccctgccacc gcgtacggtg ctggatatca atcaacttgt tgatgccatt agcctgcgcg
    35521 atctatcacc cgatcaacct atccagttaa cccagtttcg ccaggcatgg cgggtaaaag
    35581 cgggacaacg cgtcaatgtg atcgccagcg gtgatgggtt tagcgccaac gcagaaggtc
    35641 aggcgctgaa caatgcagcc gtcgcacaga atgcgcgggt gcgcatggta tcgggacagg
    35701 tagtcagcgg cgttgttgat gcagatggga atattcttat aaacctgtaa gctgttaaag
    35761 attacccgtc ccttgccgat aaataagcaa cacatgataa aagcgccctc aatgaggaat
    35821 aaaccatgag tattgatcgc acttcgcctc tgaagcctgt aagcaccgtt caaccgcgcg
    35881 aaaccactga cgcgccggta acgaacagcc gggcggcaaa aacaaccgcc tccaccagca
    35941 ccagtgtgac gttaagcgac gcgcaagcaa aactgatgca acccggcagc agtgatatca
    36001 atcttgaacg tgtcgaagcg ttaaaactgg cgattcgtaa cggtgaacta aaaatggaca
    36061 ccggcaaaat tgccgatgcg ctgatcaacg aagcgcagca agacttgcag agtaactgat
    36121 cgtatgacac gtcttgcaga gatcctcgac cagatgtccg ctgtgcttaa cgatctcaaa
    36181 acggtaatgg atcaagaaca gcaacatctc tctatggggc agatcaacgg cagccagttg
    36241 caatggatta cagaacaaaa aagctcactg ctggcgacgc tggattacct cgaacagtta
    36301 cgcaggaaag aacccaatac agcaaatagc gctgatatta gtcaacgctg gcaggaaatt
    36361 actgtgaaaa cgcagcaact acgccaaatg aatcaacata acggctggtt actggaagga
    36421 cagattgagc gcaatcaaca ggcgctggaa atgttgaaac cgcatcagga accgacgcta
    36481 tatggggcga acggtcagac ctcaacaacc catcgcggca gtaaaaagat ttcgatctga
    36541 agattatctc cggcctgcac tgcaggccgg aatgcattgt tacaccgtcc ggcgggcaaa
    36601 ttctttaact ttgaagccca gtaccgccag tgcagcgaag tacgcggcaa tccccgccag
    36661 cacgaccgcc attaaacgca gcaagcgcca gggcatggta cccaatgacc actccggcat
    36721 gatatgtaac atacctaaaa gcacgccaga catcaccagt accgccacca ccagacgcaa
    36781 cagaaacgcc atccagccag gttgcggggt aaagattttc tgcttacgca actgccagta
    36841 aagcagcgaa gcattcagac acgccgccag accaatagaa agtgacagcc cggcatgttt
    36901 caacggaccg ataaacgcca ggttcatcaa ttgcgttaaa atcagcgtaa cgatggcaat
    36961 tttcactggc gttttaatgt cctggcggga ataaaagcca ggagccaaca ctttcactac
    37021 aatcaggcca atcaaaccca ccgagtaggc aattaacgcc cgctgggtca tcagcgcatc
    37081 aaacgcggta aatttaccgt actggaacag cgaaacggtc aacggacctg aaagaatgcc
    37141 caacgcaacc gcactcggca gcgccaacag gaaacaaaga cgcagccccc agtccatcaa
    37201 acggttgtat tcatcatgat tgccactggc aaaacttttc gacagcgacg gcagcaaaat
    37261 ggtgccaagc gccacgccca gcacaccgga cggaaactcc attaagcggt cggcgtaata
    37321 catccaagac accgaaccgg aagcaagaaa cgaggcaaaa atggtgttga tgattaagga
    37381 gatctggctc acagagacgc caaggatcgc cggtcccatc tgtttcacca cgcgcattgc
    37441 tccggcatcg tggaagttaa tgcgcggcag gaccagcatg ccgatcttct tcaggtgcgg
    37501 tagctgatac accagctgca ggacgccgcc gaccgtaaca gcccacgcca gcgccagcac
    37561 cggtgggtta aagtacggtg cggcaaacag cgcgaaacca atcatgctga tgttaagcag
    37621 tgttggcgca aacgccggaa tcgagaagcg gttccacgta ttcagaatcg ctcccaccag
    37681 cgacgccagg gagatcagca agatataggg aaaggtaatc tttagtagct ggctggtcag
    37741 ggcaaatttg tcagctgtgt cagcgaagcc tggcgcggtc accatgatca cccacggtgc
    37801 ggcgagcatg ccagcgaccg tcacaaccgc cagcgcaagt gtcagcaggc cagaaacata
    37861 agagacaaag acccgcgtgg cgtcttcacc ctgcttactt ttatattccg ccagaatcgg
    37921 tacaaatgcc tgggaaaatg ccccttcggc aaagatacgg cgtaacaagt taggaagttt
    37981 aaaagcgacg aaaaaggcgt cggttgccat ccctgcgcca aagattctgg cgacaattgc
    38041 gtctcgtgcg aagccaagca cacgcgaaaa catggtcatc gagctgacgg cggccagcga
    38101 ttttaataaa ttcatcggtg ttctaatcca gacccacggg caaaacgcct gcaaggcagg
    38161 cgctgaaaga ggcgaatagt ctacctggat tatggtgaat tgctaccgcc agatgttaca
    38221 gggtttattc actcatcgca tcgcgccaga tcttgtcaac gatacgttgc gccagcactg
    38281 cctgttcgcc ggcggtttgc ggaactgtct gattttgcac acattcaatg aagtgccgcg
    38341 cacagccgac aaatccacgt tgctcaagcg tactctgcca accaggaatc ggtttatgca
    38401 ctacgccctg cccgcgctcc tcacgccatt cgcgcatatc cgtaatgtcg atgagcgcac
    38461 cgtcagtcac ggcctgcacg gtttcacgct gacttccggc acggcgatgc atacaggtgg
    38521 tgatctgcaa aggaccggcc gaaaaatggt gctcggcaaa cagcatttcg ccagcgtcgt
    38581 tagtcagtag cgtaccgcca tccagagagg ctttgccgcc cgacaaccac agcgcggtat
    38641 ccaccacatg cagataatca tccagcaacg tgaaataaag atcgtgtggc ccgacgctat
    38701 tgctacgatg tttatccatt cttagcgagg ctgcggtggc gagttgcgtt tttaactcac
    38761 cgtagagtgg tgcgaaacga cggttaaaac cgaccatcaa cgtcagtttt ttccgcgccg
    38821 ccagttccac cagccgttca gcatcgcgca gattttctgc cagcggttta tcgacacaga
    38881 catgtacccc cgcattgagt aacgtactga ccacgtcaaa gtgactggcg gtgctggaat
    38941 gtacaaaaac cgcatcgcaa ctggcggcaa ggctggataa cgaatcggca taaggaatgc
    39001 gccagctttc acaaattggc agggctttcg cgcgcgtagg cgaccaggct ccttgtaacg
    39061 tccagtcaga cgctgccgcc agcaccggta accacgcttt ttgagcaatg ccgcctaatc
    39121 ccactacgcc gatacgtaat tttttcactt taatctccca ggtgggccag caacgaatca
    39181 agacgctgtt tcagttctgc cacttcgatt tccagggctt cgacgcgggc ctgtaaatca
    39241 ccgtcaaccg cgctcgacat atccgttacc gacggctgat cctctacctc accgctgaaa
    39301 agatgcatgt agcggctttc acgtttaccc ggttcgcggg ccagacgcac cacaaaagga
    39361 ccatcttcgc gattcgccag ttgttccagc gtcgactcca cttccgccat atcgctgaat
    39421 tcatacattc gcgcggcgcg gctgcgcagt tcacccggcg tctgggcacc acgcaataac
    39481 aacgtggtga ttaacgccac ttctgcggcg ctcagtttca gatcgccaaa ttctgaatta
    39541 caaaaacgtt gctcatattt ggtgacccga ttaccaaaac cgctcactgt gcgtagataa
    39601 tgacgtttga ccagattatc cagctgttcc tgcacttcag attcactcag attcatgacc
    39661 ggttcacggt tcgttttctg attacaggcc gtgactacac cattgactga gagcgggtat
    39721 tgctccggcg tggtcacctg cttttccagc aagcaaccaa tcacacgagc ttccagggca
    39781 gtaagttgat atttcatgag attctcctta gcggccgggc gtccagtctg gggtagttaa
    39841 tgccgtcagt acgtgatcgc gccattgtcc atcaatcaac agatagtctt tcgcatagcc
    39901 ttctttttca aaacccagtc gcgccagtaa atcaccgctg cgtttattgt gcggcatata
    39961 attagccata atgcgatgaa tatgttgggt gcgctgcata taacgaatgg ctgcggtcag
    40021 ggcttcaaac atgagtcctt tgccctgcca tttttgccca atcgaataac cgagatagca
    40081 ggcatgaaaa gagccgcgaa caacattgga aaaattggca acgccaataa tctctttttc
    40141 gtccgggtcg aataaaccaa agtagaaagc tgaaccttgt ttatgaaatt cgttaatcat
    40201 ccccagcctg gcctgccagc ctgatggata gcagtggctt tcgtcgcgca ctggctccca
    40261 gggcttgagg aaatggcgat tctctgcgta ataatccgcc agacgccagg catcacgatc
    40321 atgcaccaga cgcacgacca gtcggtctgt ggttaagcgc acttttggca cgttactgcg
    40381 atagccaaac attctatacc tactccttcc cgtaacgtct ttaatacgct gtaagtatta
    40441 ctatacctgc gcaaccagac cctgtgaaaa gtccaaaatg ccattttctg cattattcac
    40501 atatttgata aaaactctct atcaaagcga cgttttgata aaaaaatatt gtccccgaag
    40561 tgatgggaaa agtcttagcg gcaatgcaga atagaaagtg ctcatctttt atttcccggg
    40621 aggggaaatg tcccgcgtat cgcaggcgag gaacctgggt aaatatttcc tgctcatcga
    40681 taatatgctg gtcgtgctgg ggttctttgt tgtcttcccg ctgatctcta tccgcttcgt
    40741 tgatcaaatg ggctgggccg ccgtcatggt cggtattgct ctcggtctac gccagtttat
    40801 tcagcaaggt ctgggtattt tcggcggcgc aattgccgac cgctttggtg ccaaaccgat
    40861 gattgttacc ggtatgctga tgcgcgccgc cggattcgcc acaatgggta tcgcccacga
    40921 accgtggcta ttgtggtttt catgcctgct ctcgggactc ggcggcacgt tgtttgatcc
    40981 gccgcgttcg gcgctggtgg tgaaactaat ccgtccacaa caacgtggtc gttttttctc
    41041 gctgttgatg atgcaggaca gtgccggtgc ggtcattggc gcattgttgg ggagctggct
    41101 gttgcaatac gactttcgcc tggtctgcgc cacaggggcc gttttgtttg tgctgtgtgc
    41161 ggcgttcaat gcgtggttgt taccggcatg gaaactctcc accgtacgca cgcccgttcg
    41221 cgaaggcatg acccgcgtga tgcgtgacaa gcgttttgtc acctatgtcc tgacgctggc
    41281 gggttactac atgctggctg tacaagtgat gctgatgctg ccaattatgg tcaacgacgt
    41341 ggctggcgca ccctctgccg ttaaatggat gtatgccatt gaagcgtgtc tgtcgttaac
    41401 gttgctctac cctatcgccc gctggagtga aaagcatttt cgtctggaac accggttgat
    41461 ggctgggctg ttgataatgt cattaagcat gatgccggtg ggcatggtca gcggcctgca
    41521 acaacttttc accctgattt gtctgtttta tatcgggtcg atcattgccg agcctgcgcg
    41581 tgaaacctta agtgcttcgc tggcggacgc aagagctcgc ggcagctata tggggtttag
    41641 ccgtctgggt ctggcgattg gcggcgctat tggttatatc ggtggtggct ggctgtttga
    41701 cctgggcaaa tcggcgcacc agccagagct tccgtggatg atgctgggaa ttattggcat
    41761 cttcactttc cttgcgctgg gttggcagtt tagtcagaaa cgcgccgcgc gtcgtttgct
    41821 ggaacgcgac gcctgatttg atactccccc ctgactcctc catactgaaa gtgaactgac
    41881 agccaaatgg aggagtcacg tgaagctata catttacgat cactgccctt actgcctcaa
    41941 agcccgcatg attttcggcc tgaaaaatat ccccgtcgaa ttacatgttc tgctcaacga
    42001 cgacgcagaa acacccaccc ggatggtcgg tcaaaaacag gttcccattc tgcaaaaaga
    42061 tgacagccgc tatatgccag aaagcatgga tatcgttcac tatgtcgata aactcgacgg
    42121 caaaccgtta ctgaccggca aacgttcccc tgccattgaa gagtggctgc gcaaggtcaa
    42181 tggctacgcc aacaaactgc tgttgccgcg ttttgccaaa tcggcatttg atgagttttc
    42241 tactcccgcc gcgcgcaaat atttcgtcga caagaaagag gccagcgcgg gtaattttgc
    42301 cgacctgctg gcccactctg acggtctgat taagaatatc agcgatgatt tgcgtgcgct
    42361 ggacaaactg atcgtcaaac cgaacgccgt gaatggcgaa ctttcggaag atgatattca
    42421 gctatttccg ctactgcgta atctgacgct ggtagccgga atcaactggc caagccgcgt
    42481 tgctgattac cgcgataata tggcgaaaca gacacaaatc aatttgttat catcaatggc
    42541 gatttaatcc tgacccgccg cggcagcctg tattcgcggg ttgccgccgt tttcagtttt
    42601 ttctgctggc gagtttatcg ctggtgacac atgctgcgtt gtgtcaggat attgacgcag
    42661 atttcatgtc gaggactacc atgaacaagt ttttatttgc cgctgcattg atcgtcagtg
    42721 gcctgctcgt tggctgtaat caactcaccc aatacaccat caccgaacaa gaaattaacc
    42781 agtcgcttgc gaaacataat aatttctcaa aagatatcgg tttacccggc gtggctgacg
    42841 cccatattgt tctgacaaac ttgaccagcc aaattggtcg cgaagagccg aataaggtta
    42901 ccctaaccgg agacgccaat ctggacatga actccctgtt cggtagtcag aaagcgacca
    42961 tgaaactgaa gctgaaagcg ctgcccgtgt ttgataaaga aaaaggtgcg atcttcctga
    43021 aagagatgga agtggtcgat gcgacggtac aaccggaaaa aatgcaaacg gtgatgcaaa
    43081 cgttgcttcc ctatttgaac caggcattac gcaattactt taaccagcaa cctgcttacg
    43141 tcctgcgcga agatggcagt cagggcgaag caatggcgaa aaaactggcg aaaggcattg
    43201 aagtgaagcc aggcgaaatt gtcattccgt ttacggatta atgacgaggg cgcattcgcg
    43261 ccctttattt ttcgtgcaaa ggaaaacgtt tccgcttatc ctttgtgtcc ggcaaaaaca
    43321 tcccttcagc cggagcatag agattaatga ctgcaccatc ccaggtatta aagatccgcc
    43381 gcccagacga ctggcacctt cacctccgcg atggcgacat gttaaaaact gtcgtgccgt
    43441 ataccagcga aatttatgga cgggctatcg taatgcccaa tctggctccg cccgtgacca
    43501 cagttgaggc tgccgtggcg tatcgccaac gtattcttga tgccgtacct gccgggcaca
    43561 atttcacccc attgatgacc tgttatttaa cagattcgct ggatcctaat gagctggagc
    43621 gcggatttaa cgaaggcgtg ttcaccgctg caaaacttta tccggcaaac gcaaccacta
    43681 actccagcca cggcgtgacg tcaattgacg caatcatgcc ggtacttgag cgcatggaaa
    43741 aaatcggtat gccgctactg gtgcatggtg aagtgacaca tgcagatatc gacatttttg
    43801 atcgtgaagc gcgctttata gaaagcgtga tggaacctct gcgccagcgc ctgactgcgc
    43861 tgaaagtcgt ttttgagcac atcaccacca aagatgctgc cgactatgtc cgtgacggaa
    43921 atgaacggct ggctgccacc atcactccgc agcatctgat gtttaaccgc aaccatatgc
    43981 tggttggtgg cgtgcgtccg cacctgtatt gtttacccat cctcaaacgc aatattcacc
    44041 aacaggcatt gcgtgaactg gtcgccagcg gttttaatcg agtattcctc ggtacggatt
    44101 ctgcgccaca tgcacgtcat cgcaaagaga gcagttgcgg ctgcgcgggc tgcttcaacg
    44161 ccccaaccgc gctgggcagt tacgctaccg tctttgaaga gatgaatgct ctgcagcact
    44221 ttgaagcatt ctgttctgta aacggcccgc agttctatgg cttgccggtc aacgacacat
    44281 tcatcgaact ggtacgtgaa gagcaacagg ttgctgaaag catcgcactg actgatgaca
    44341 ccctggtgcc attcctcgcc ggggaaacgg tacgctggtc cgttaaacaa taaaaattgt
    44401 agcttcctgt tgtcattagg ttattttacc tgtataaata accagtatat tcaacagggg
    44461 gctattatgc gaattgaagt caccatagcg aaaacttctc cattgccagc tggggctatt
    44521 gacgccctgg ctggcgaact ttcccgccgt attcagtatg cgtttcctga taatgaaggc
    44581 cacgtatcgg tacgttatgc cgcagcgaat aatttatcgg ttattggcgc aacaaaagaa
    44641 gataaacagc gcattagcga aattctccag gaaacgtggg aaagcgccga tgactggttt
    44701 gtcagcgaat aatatgcagt gatttttgtt tttgccgggt cgccccggct tttttatttt
    44761 acttacaaat tttgagtaca aaatttctac acttcctcaa aataattgca caatatgcta
    44821 aaaaaatatg aatgacatgc tgttttattg ttcatataaa tcttaaaagc agaaatttat
    44881 ctgttccccc ctttaaaaat tcggtgaata cccttactta ttggtatact gaagttgctt
    44941 cagataaaaa cgcattgaac ctcgaataac gttgtctagt aacacgaatt agggggccat
    45001 gatggaaaaa aataatgaag tcattcagac tcatccgctc gtagggtggg acatcagcac
    45061 cgttgatagc tatgatgcgc tgatgttgcg tttgcactac cagaccccaa ataagtccga
    45121 gcaggaaggg actgaagttg gtcagacgct ctggttaacc actgatgttg ccagacaatt
    45181 tatttcgata ttagaagcag gaatcgccaa aattgaatcc ggtgatttcc cggtaaacga
    45241 gtatcggcgt cattaacatg acatgaccat ccacccaaaa ggcacctcac cggtgccttt
    45301 accatttctg ggttgtatat ctgcattcag ttaattactc tgcttaaacc atgagagcag
    45361 agagaaaaaa atgaaatacg atctcatcat tattggcagc ggttccgtag gcgctgccgc
    45421 cgggtattat gcaacccgcg ccggtttaaa cgtgctaatg accgacgccc atatgccacc
    45481 gcatcaacac ggcagccacc acggcgatac gcgattaatt cgccatgctt atggtgaagg
    45541 cgaaaagtat gtcccgttgg tcctccgcgc gcaaacgctg tgggatgaac tttcccgcca
    45601 caacgaagat gatcccattt ttgtacgctc tggtgtcatt aacctcggcc cggctgactc
    45661 cgcatttctc gccaacgtcg cccacagcgc cgaacagtgg caactcaacg ttgaaaagct
    45721 cgatgcgcaa gggattatgg cccgctggcc agaaatacgc gtcccggaca actacatcgg
    45781 cttatttgag actgattccg gttttttgcg cagcgaactg gcgattaaaa cctggatcca
    45841 actggcgaag gaagcgggct gtgcgcaact gttcaactgc ccggtcaccg caattcatca
    45901 tgacgatgat ggcgtaacta ttgaaacggt tgacggtgag tatcaggcga aaaaagcgat
    45961 tgtctgcgcg ggaacatggg taaaagacct gctcccggag ctgcctgtcc agcccgtacg
    46021 taaagtattt gcctggtatc aggccgatgg ccgctatagc gtgaagaata aattcccggc
    46081 gtttaccggt gaactgccca atggcgatca atattatggt tttccggcag aaaacgacgc
    46141 gttgaagatt ggcaaacata acggaggcca ggttatccat tcagcggatg aacgtgttcc
    46201 gtttgcggaa gtggtcagcg atggttcgga agccttcccg ttcttgcgca atgtattgcc
    46261 gggtatcggt tgctgcctgt acggcgctgc ctgcacctat gataattcgc ctgacgaaga
    46321 ttttattatc gataccctac ccggccacga taatacactg ctcattaccg gcctgagtgg
    46381 gcacggtttt aaatttgcgt cagttttagg ggaaatagct gccgattttg cgcaagacaa
    46441 aaaaagcgat tttgatttga cgccattcag gctttcccgc ttccaataat catcattacg
    46501 gcctccagaa tgaggccgtt tttacaggag tatttaatgc gtcggctgtt gcactatctc
    46561 atcaataata ttcgcgagca tctgatgctt tatctttttc tctggggatt gttggcaatt
    46621 atggatctga tctatgtgtt ttatttttag ttttccgcac tgacataaac tgacaaatat
    46681 ttaatctccc tttcatttag cgtgaaaaat tgaatgagat tattcctgct tctacagcac
    46741 tcaccagaca tctc
//
LOCUS       DBANUU010000022        45460 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000022 DBANUU010000000
VERSION     DBANUU010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.022"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            4..885
                     /gene="yieE"
                     /locus_tag="ACIE68_001191"
     CDS             4..885
                     /gene="yieE"
                     /locus_tag="ACIE68_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFX5907539.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            1090..2559
                     /gene="lysP"
                     /locus_tag="ACIE68_001192"
     CDS             1090..2559
                     /gene="lysP"
                     /locus_tag="ACIE68_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFX5907540.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2853..4844
                     /gene="cirA"
                     /locus_tag="ACIE68_001193"
     CDS             2853..4844
                     /gene="cirA"
                     /locus_tag="ACIE68_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFX5907541.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSFNYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(4876..5712)
                     /gene="yeiG"
                     /locus_tag="ACIE68_001194"
     CDS             complement(4876..5712)
                     /gene="yeiG"
                     /locus_tag="ACIE68_001194"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFX5907542.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5970..6638
                     /gene="folE"
                     /locus_tag="ACIE68_001195"
     CDS             5970..6638
                     /gene="folE"
                     /locus_tag="ACIE68_001195"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFX5907543.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6655..7812
                     /gene="yeiB"
                     /locus_tag="ACIE68_001196"
     CDS             6655..7812
                     /gene="yeiB"
                     /locus_tag="ACIE68_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFX5907544.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLVGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            7954..8994
                     /gene="galS"
                     /locus_tag="ACIE68_001197"
     CDS             7954..8994
                     /gene="galS"
                     /locus_tag="ACIE68_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFX5907545.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            9274..10272
                     /gene="mglB"
                     /locus_tag="ACIE68_001198"
     CDS             9274..10272
                     /gene="mglB"
                     /locus_tag="ACIE68_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFX5907546.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10333..11853
                     /gene="mglA"
                     /locus_tag="ACIE68_001199"
     CDS             10333..11853
                     /gene="mglA"
                     /locus_tag="ACIE68_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFX5907547.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11869..12879
                     /gene="mglC"
                     /locus_tag="ACIE68_001200"
     CDS             11869..12879
                     /gene="mglC"
                     /locus_tag="ACIE68_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFX5907548.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(12950..14185)
                     /gene="preA"
                     /locus_tag="ACIE68_001201"
     CDS             complement(12950..14185)
                     /gene="preA"
                     /locus_tag="ACIE68_001201"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFX5907549.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPIIGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(14179..15417)
                     /gene="preT"
                     /locus_tag="ACIE68_001202"
     CDS             complement(14179..15417)
                     /gene="preT"
                     /locus_tag="ACIE68_001202"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFX5907550.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKMLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSNVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(15736..15975)
                     /gene="yeiS"
                     /locus_tag="ACIE68_001203"
     CDS             complement(15736..15975)
                     /gene="yeiS"
                     /locus_tag="ACIE68_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFX5907551.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(15978..16697)
                     /gene="sanA"
                     /locus_tag="ACIE68_001204"
     CDS             complement(15978..16697)
                     /gene="sanA"
                     /locus_tag="ACIE68_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFX5907552.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(16847..17731)
                     /gene="cdd"
                     /locus_tag="ACIE68_001205"
     CDS             complement(16847..17731)
                     /gene="cdd"
                     /locus_tag="ACIE68_001205"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFX5907553.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(17861..18556)
                     /gene="yohK"
                     /locus_tag="ACIE68_001206"
     CDS             complement(17861..18556)
                     /gene="yohK"
                     /locus_tag="ACIE68_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFX5907554.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(18553..18951)
                     /gene="yohJ"
                     /locus_tag="ACIE68_001207"
     CDS             complement(18553..18951)
                     /gene="yohJ"
                     /locus_tag="ACIE68_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFX5907555.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            19190..20137
                     /gene="dusC"
                     /locus_tag="ACIE68_001208"
     CDS             19190..20137
                     /gene="dusC"
                     /locus_tag="ACIE68_001208"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFX5907556.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATGLFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(20510..20593)
                     /gene="yohP"
                     /locus_tag="ACIE68_001209"
     CDS             complement(20510..20593)
                     /gene="yohP"
                     /locus_tag="ACIE68_001209"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFX5907557.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            20817..22253
                     /gene="mdtQ"
                     /locus_tag="ACIE68_001210"
     CDS             20817..22253
                     /gene="mdtQ"
                     /locus_tag="ACIE68_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFX5907558.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            22306..23067
                     /gene="yohF"
                     /locus_tag="ACIE68_001211"
     CDS             22306..23067
                     /gene="yohF"
                     /locus_tag="ACIE68_001211"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFX5907559.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATYEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(23197..23775)
                     /gene="yohD"
                     /locus_tag="ACIE68_001212"
     CDS             complement(23197..23775)
                     /gene="yohD"
                     /locus_tag="ACIE68_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFX5907560.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            23945..24532
                     /gene="yohC"
                     /locus_tag="ACIE68_001213"
     CDS             23945..24532
                     /gene="yohC"
                     /locus_tag="ACIE68_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFX5907561.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            24706..25635
                     /gene="pbpG"
                     /locus_tag="ACIE68_001214"
     CDS             24706..25635
                     /gene="pbpG"
                     /locus_tag="ACIE68_001214"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFX5907562.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVETTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAGQTAQND"
     gene            complement(25673..27388)
                     /gene="dld"
                     /locus_tag="ACIE68_001215"
     CDS             complement(25673..27388)
                     /gene="dld"
                     /locus_tag="ACIE68_001215"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFX5907563.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            27584..29881
                     /gene="bglX"
                     /locus_tag="ACIE68_001216"
     CDS             27584..29881
                     /gene="bglX"
                     /locus_tag="ACIE68_001216"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFX5907564.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            30092..31009
                     /gene="osmF"
                     /locus_tag="ACIE68_001217"
     CDS             30092..31009
                     /gene="osmF"
                     /locus_tag="ACIE68_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFX5907565.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            31016..32173
                     /gene="yehY"
                     /locus_tag="ACIE68_001218"
     CDS             31016..32173
                     /gene="yehY"
                     /locus_tag="ACIE68_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFX5907566.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGFGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            32166..33092
                     /gene="yehX"
                     /locus_tag="ACIE68_001219"
     CDS             32166..33092
                     /gene="yehX"
                     /locus_tag="ACIE68_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFX5907567.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVMNTQGQPCGTLHFQDLLEEA"
     gene            33097..33828
                     /gene="yehW"
                     /locus_tag="ACIE68_001220"
     CDS             33097..33828
                     /gene="yehW"
                     /locus_tag="ACIE68_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFX5907568.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(33809..33916)
                     /gene="yohO"
                     /locus_tag="ACIE68_001221"
     CDS             complement(33809..33916)
                     /gene="yohO"
                     /locus_tag="ACIE68_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFX5907569.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(33976..34707)
                     /gene="mlrA"
                     /locus_tag="ACIE68_001222"
     CDS             complement(33976..34707)
                     /gene="mlrA"
                     /locus_tag="ACIE68_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFX5907570.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            34929..36614
                     /gene="btsS"
                     /locus_tag="ACIE68_001223"
     CDS             34929..36614
                     /gene="btsS"
                     /locus_tag="ACIE68_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFX5907571.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            36611..37330
                     /gene="btsR"
                     /locus_tag="ACIE68_001224"
                     /gene_synonym="yehT"
     CDS             36611..37330
                     /gene="btsR"
                     /locus_tag="ACIE68_001224"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFX5907572.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            37377..37847
                     /gene="yehS"
                     /locus_tag="ACIE68_001225"
     CDS             37377..37847
                     /gene="yehS"
                     /locus_tag="ACIE68_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFX5907573.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(37888..38349)
                     /gene="yehR"
                     /locus_tag="ACIE68_001226"
     CDS             complement(37888..38349)
                     /gene="yehR"
                     /locus_tag="ACIE68_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFX5907574.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(38474..40474)
                     /gene="yehQ"
                     /locus_tag="ACIE68_001227"
     CDS             complement(38474..40474)
                     /gene="yehQ"
                     /locus_tag="ACIE68_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFX5907575.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRALQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(40471..41607)
                     /gene="yehP"
                     /locus_tag="ACIE68_001228"
     CDS             complement(40471..41607)
                     /gene="yehP"
                     /locus_tag="ACIE68_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFX5907576.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(41600..43879)
                     /gene="yehM"
                     /locus_tag="ACIE68_001229"
     CDS             complement(41600..43879)
                     /gene="yehM"
                     /locus_tag="ACIE68_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFX5907577.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPERGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEVDDSPDTQEESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRLREAFMARWIGWAMQQNNGDVLVVCGGWHAPVLAKMWRECPQEIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAAHGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            complement(43890..44978)
                     /gene="yehL"
                     /locus_tag="ACIE68_001230"
     CDS             complement(43890..44978)
                     /gene="yehL"
                     /locus_tag="ACIE68_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFX5907578.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT        10840 a        11443 c        12170 g        11007 t
ORIGIN      
        1 actatgcaca tcaccctccg gcagttggaa gtttttgcag aagtattgaa aagtggatca
       61 accacccagg cgtcggtgat gctggcgttg tcgcaatcag cagtgagcgc agccttgacc
      121 gacctggaag ggcagcttgg cgttcaactg tttgatcgcg tggggaaaag actggttgtt
      181 aatgaacacg ggcggctgct ctatccgcga gcgttggcac tgcttgaaca ggcggttgaa
      241 atcgaacaac tgtttcgcga agacaacggc gcgattcgta tctatgccag tagtaccatc
      301 ggtaactaca ttctgcctgc agttatcgcc cgttatcgcc atgattatcc gcagttgccg
      361 attgaactta gcgttgggaa tagccaggat gtgatgcaag cggtgctgga tttccgcgtt
      421 gatattggct ttattgaagg accgtgccac agcactgaaa tcatttctga accgtggctg
      481 gaagacgagc tggtggtttt cgccgcgccg acttcgccgt tggcccgtgg tccggtcacc
      541 ttagagcagc tggccgctgc gccgtggatc ctgcgtgaac gcggttccgg cacgcgggag
      601 attgtcgatt atctattgct gtcacattta ccgaagtttg agatggcgat ggaattaggt
      661 aactccgagg caatcaaaca tgcggtgcgt catgggttgg gaattagttg cctgtcgcga
      721 cgtgtgattg aagatcaatt gcaggcaggc acattaagtg aagttgcggt ccctctcccg
      781 cgcctgatgc gtacgttgtg gcgtatacat catcggcaaa aacacctttc caacgcgcta
      841 cggcgctttc tggactattg cgatcccgcg aatgtaccgc gttaaggtgc tgtacaagaa
      901 catgctggtg ctgtgtcgat ttcgtgacgc agcgccctca gcatgcattc gccagaaaag
      961 agattggctg ctttacttat aatccctggg cgatcatgaa ggtgtcttat aaccatgtat
     1021 ttctgccgga aggattgcca atcgtctgct acaatcgcgc ctcattttta agatggatag
     1081 catttttgta tggtttccga aactaaaacc acagaagcgc cgggcttacg ccgtgaatta
     1141 aaggcgcgtc acctgacgat gattgccatt ggcggttcca tcggtacagg tctttttgtt
     1201 gcctctggcg caacgatttc tcaggcaggt ccgggcgggg cattgctctc gtatatgctg
     1261 attggcctga tggtttactt cctgatgacc agtctcggtg aactggctgc atatatgccg
     1321 gtttccggtt cgtttgccac ttacggtcag aactatgttg aagaaggctt tggcttcgcg
     1381 ctgggctgga actactggta caactgggcg gtgactatcg ccgttgacct ggttgcagct
     1441 cagctggtca tgagctggtg gttcccggat acaccgggct ggatctggag tgcgttgttc
     1501 ctcggcgtta tcttcctgct gaactacatc tcagttcgtg gctttggtga agcggaatac
     1561 tggttctcac tgatcaaagt cacgacagtt attgtcttta tcatcgttgg cgtgctgatg
     1621 attatcggta tcttcaaagg cgcgcagcct gcgggctgga gcaactggac aatcggcgaa
     1681 gcgccgtttg ctggtggttt tgcggcgatg atcggcgtag ctatgattgt cggcttctct
     1741 ttccagggaa ccgagctgat cggtattgct gcaggcgagt ccgaagatcc ggcgaaaaac
     1801 attccacgcg cggtacgtca ggtgttctgg cgaatcctgt tgttctatgt gttcgcgatc
     1861 ctgattatca gcctgattat tccgtacacc gatccgagcc tgctgcgtaa cgatgttaaa
     1921 gacatcagcg ttagtccgtt caccctggtg ttccagcacg cgggtctgct ctctgcggcg
     1981 gcggtgatga acgcagttat tctgacggcg gtgctgtcag ccggtaactc cggtatgtat
     2041 gcgtctactc gtatgctgta caccctggcg tgtgacggta aagcgccgcg cattttcgct
     2101 aaactgtcgc gtggtggcgt gccgcgtaat gcgctgtatg cgacgacggt gattgccggt
     2161 ctgtgcttcc tgacctccat gtttggcaac cagacggtat acctgtggct gctgaacacc
     2221 tccgggatga cgggttttat cgcctggctg gggattgcca ttagccacta tcgcttccgt
     2281 cgcggttacg tattgcaggg acacgacatt aacgatctgc cgtaccgttc aggtttcttc
     2341 ccactggggc cgatcttcgc attcattctg tgtctgatta tcactttggg ccagaactac
     2401 gaagcgttcc tgaaagatac cattgactgg ggcggcgtag cggcaacgta tattggtatc
     2461 ccgctgttcc tgattatttg gttcggctac aagctgatta aaggaactca cttcgtacgc
     2521 tacagcgaaa tgaagttccc gcagaacgat aagaaataag tttcctccct tccttgctaa
     2581 gccctctcaa ccgagagggc tttttcaatt ccatttccct gacaaatcat gcggatataa
     2641 aatttaacat ttggattgat aattgttatc gtttgcatta tcgttacgcc acaatcaaaa
     2701 aaggctgaca aatcagaggc tgttccggct ttctgggatg atcacctgca taaaaaataa
     2761 gtccaccgcg atgctgccgt acgcaagggg acgtgaagaa gatgtgagcg ataacccatt
     2821 ttattttcgt agttacctca tggagatatg gaatgtttag gttgaaccct ttcgtacggg
     2881 tcgggctgtg tttgtccgct atttcttgtg catggcctgt gttagcggtc gatgatgatg
     2941 gcgaaacgat ggttgtcact gcatcttccg tggaacaaaa tcttaaagat gcaccagcca
     3001 gtatcagcgt cattacccag gaagacctgc agcgaaaacc ggtacagaat ctgaaggatg
     3061 tcctcaaaga agtgcctggc gtacaactga cgaacgaagg ggataaccgt aagggcgtta
     3121 gtattcgtgg tctggacagc agctacaccc tgattcttgt cgacggtaaa cgcgttaact
     3181 cccgcaatgc cgtcttccgc cacaatgatt tcgatctgaa ctggatcccg gtcgattcca
     3241 tcgaacgtat tgaagtggtt cgtggcccga tgtcgtcgct gtacggctcc gatgcgctcg
     3301 gcggtgtagt gaatatcatc accaaaaaaa tcggtcagaa atggtcgggt accgttaccg
     3361 tcgataccac cattcaggaa catcgcgatc gcggtgatac ctataacggt cagttcttta
     3421 ccagcggacc attaattgac ggcgtgctgg gaatgaaagc ttacggcagc ctggcaaaac
     3481 gtgaaaagga tgacccgcaa aactcaacga ccaccgatac cggagaaacg ccgcgtattg
     3541 aaggattctc cagccgcgac ggtaatgtcg aatttgcctg gacaccgaat caaaatcacg
     3601 attttactgc cggatacggt ttcgaccgtc aggatcgtga ttccgactcg ctggacaaaa
     3661 accgcctgga acgccagaac tactccgtca gccataatgg gcgttgggat tacggcacca
     3721 gcgaactgaa atactacggt gagaaagtcg agaacaaaaa ccctggcaac agcagcccga
     3781 taacttccga aagcaatacg gtcgacggca aatacacgtt gccgctgacg gcgattaatc
     3841 agtttctcac ggttggcggt gaatggcgtc acgacaaact tagcgatgcg gtgaacctga
     3901 ccgggggaac cagctccaaa acgtctgcca gccagtacgc gctgtttgtg gaagatgaat
     3961 ggcggatctt cgagccgctg gcgctgacga ccggcgtgcg tatggacgat cacgaaacct
     4021 acggtgaaca ctggagtccg cgtgcctacc tggtttataa cgccaccgac accgtaacgg
     4081 tgaaaggggg ctgggcgacg gcatttaaag caccttctct gttgcaactt agccctgact
     4141 ggacgagcaa ttcctgccgt ggcgcatgta agattgtggg tagcccggat ctgaaaccag
     4201 aaaccagcga aagttgggag ctggggcttt actacatggg tgaagaaggc tggcttgaag
     4261 gggttgaatc cagcgttacc gttttccgta acgatgtgaa agatcgtatc agcatcagcc
     4321 gtacgtctga cgtcaacgct gcaccgggct accaaaactt tgtcggtttt gagacgggcg
     4381 ctaacggacg gcgcataccg gtatttagct actacaacgt taacaaagct cgtattcagg
     4441 gcgtggaaac cgaactgaaa attccgttca acgatgaatg gaaactgtca ttcaactaca
     4501 cctacaacga tggtcgtgat gtcagcaacg gcgaaaacaa accgctatcc gatctgccgt
     4561 tccataccgc taacggtacg ctggactgga aaccgctggc gctggaagac tggtcattct
     4621 atgtttctgg tcactatacc gggcagaaac gagccgacag cgcgacggct aaaacacctg
     4681 gcggttatac catctggaat accggcgcgg cctggcaggt gactaaagac gtcaaactgc
     4741 gcgcaggcgt gctgaacctt ggcgacaagg atctcagccg tgatgactac agctataacg
     4801 aagacggacg tcgttacttt atggcagtgg attatcgctt ctgatgagaa gatgcccggc
     4861 gaaccgggcg gactttcact tcagtaaata ctgcgcatgg aagcgcaggt gatcctctat
     4921 aaaagaggcg atgaagtagt aactgtgatc atatcccggc tgaatacgca gcgtcatcgg
     4981 ccacgctttc tggcgcgcgg cttctgccag aactgcgggt tgcaactgat cggcaagaaa
     5041 ctgatcatta tcaccttgat cgataagcgt cgggatcgca tcctgcgcgt tactggcata
     5101 catcagcgcg caactgtccc attccagcca tgcatttttg tcctcaccta aatagcggct
     5161 aaacgctttg atcccccacg ggacgctgca cggattcaca atgggcgcaa aggcggaaac
     5221 gctggtgtat ttgcccgggt ttttcagcgc cataatcagc gcaccgtgac cacccattga
     5281 atgaccgcta atggcgcagc ggtcgctgac attaaattgc gactgaacca gcgccggtaa
     5341 ttcatcgcgc agataatcat acatccggta atgcgtcgcc cacggcggtt gcgtggcatt
     5401 aagataaaag cctgcgccct ggcctaaatc gtagccatcg tcgttggcaa cctgttcgcc
     5461 gcgcgggctg gtgtctggca tcaccagtac aatccccagt tccgccgcta cccgctgggc
     5521 acccgccttg gtggtgaagt tctcgtcatt gcaggttaat ccggaaagcc agtacagcac
     5581 tggtggtggg gtgtgatcac gaggtggtgg gagaaagata ctgaacgtca tcgggcagtt
     5641 taaggtactg gagtcgtgtc gccagcgttg ctgccagcct tcaaaacagc ggtgctcttc
     5701 gagcatttcc atgcgtggct ccttgttgtg ttgtttgcaa tttcttcatc ataccgttta
     5761 ttcaatgtgc tgtgagtaac tttcacttcc gtatttgcat aacgatgttt taacatctgc
     5821 tgatgaaagg cagcggcaat tacaataatt atcgctgtga atactggatt atgtgcgccg
     5881 cctcacgcac aataatcagg ctgtaaatca gcttaataac tttgccccca cgcagggcgg
     5941 aggcgtcaca cctgcaggag aaatcataaa tgccatcact cagtaaagaa gcggccctgg
     6001 ttcatgaagc gttagttgcg cgaggactgg aaacaccgct gcgcccgccc gtgcatgaaa
     6061 tggataacga aacgcgcaaa agccttattg ctggtcatat gaccgaaatc atgcagctgc
     6121 tgaatctcga cctggctgat gacagtttga tggaaacgcc gcatcgcatc gctaaaatgt
     6181 atgtcgatga aattttctcc ggtctggatt acgccaactt cccgaaaatc accctcattg
     6241 aaaacaaaat gaaggtcgat gaaatggtca ccgtgcgcga tatcactctg accagtacct
     6301 gtgaacacca ttttgttacc atcgatggca aagcgacggt ggcttatatc ccgaaagatt
     6361 cggtgatcgg tctgtcaaaa attaaccgta tcgtgcagtt ctttgcccag cgtccgcagg
     6421 tgcaggaacg tctgacgcag caaattctta ttgcgctaca aacgctgctg ggcaccaata
     6481 acgtggctgt ctcgatcgac gcggtgcatt actgcgtgaa ggcgcgtggc atccgcgatg
     6541 caaccagtgc cacgacaacg acctctcttg gtggattgtt caaatccagt cagaatacgc
     6601 gccacgagtt tctgcgcgct gtgcgtcatc acaactgatt aaaaggcagg aaccatggag
     6661 cgcaacgtca cgctcgattt tgttcgcggc gtcgccattc tggggatcct gctattaaac
     6721 atcagcgcct ttgggctacc aaaggcggct tatctcaatc ccgcctggta cggcgctatt
     6781 acgccgcagg atgcatggac ctgggcattt cttgatctcg tcggccaggt gaaattcctc
     6841 acgctttttg cgctgctgtt tggtgcgggc ctgcaaatgt tgctgccccg tggcagacgc
     6901 tggatccagt cgcggttaac gctgttagtc ttgctgggtt ttattcacgg tttattgttc
     6961 tgggacggcg atattctgct ggcttacggg ctggtgggct taatctgctg gcggctggtg
     7021 cgcgatgcgc catcggtaaa aagcctgttt aatacaggcg tcatgcttta tctggtgggg
     7081 cttggcgttt tgctgttatt ggggctgatt tccgacagcc agaccagccg cgcctggacg
     7141 ccggatgcat cggctatttt gtatgaaaaa tactggaagc ttcacggcgg cgttgaagcg
     7201 atcagtaatc gtgccgatgg tgttggcaac agtttactgg cactgggcgc acagtatggc
     7261 tggcaactgg cagggatgat gctcattggt gcggcgctga tgcgcagcgg ctggctgaaa
     7321 gggcagttca gcttacgtca ctatcgtcgt actggttttg tgctggtggc gattggggtg
     7381 accattaacc ttcctgccat cgccctgcaa tggcagctgg actgggcgta tcgctggtgt
     7441 gccttcttac ttcaaatgcc gcgggaactg agtgcgccgt ttcaggcgat tggctatgcg
     7501 tcgctgtttt atggcttctg gccgcaattg agccgcttta agctggtgct tgcgatcgcc
     7561 tgcgtcggac ggatggcgct gaccaactat ctattgcaaa cgctgatttg taccatgctt
     7621 ttttaccacc tcggtttgtt tatgcatttt gatcgcctgg agctgctggc gtttgttatt
     7681 ccggtatggc tggcgaatat cctcttctct gttatctggc tgcgttactt ccgccagggg
     7741 ccggtggaat ggctctggcg tcagttaact ttgcgtgctg ccggaacggc aatatctaaa
     7801 acatcaagat aacgatctgg atcacattcg ttaacaaaac ggctgtaacc gtttccattg
     7861 ctgtgactcg attcacgaag tcctgtattc agtgctgaca aaatagccgc cagcaagcag
     7921 tcatttactg caatctcata acaggtagtg aatatgatca ccattcgtga tgtagcgcgt
     7981 caggctggcg tctctgtggc aacggtttcc cgggtgctca ataacagcac gctggtcagt
     8041 gccgacacgc gtgaagcagt aatgaaagcc gtgagtgagc tggattatcg gccaaatgcc
     8101 aatgcccagg cgctggcaac tcaggttagc gataccattg gcgtggtggt gatggacgtt
     8161 tctgatgctt ttttcggcgc gctggtaaaa gcggtggatc tggtcgccca gcagcatcag
     8221 aaatacgtgc taatcggcaa tagctatcat gaagcggaaa aagagcgtca cgccattgag
     8281 gtgttaattc gccagcgttg taatgcgttg attgttcact caaaagcatt gagtgacgat
     8341 gaactggcgc aatttatgga taacattccc ggtatggtgt taatcaaccg cgttgtgccg
     8401 gggtacgccc atcgttgcgt ttgcctggat aatctcagcg gtgcccgaat ggcgacgcgc
     8461 atgttgctga ataacggtca tcaacgtatt ggttatcttt cttccagcca cggcattgaa
     8521 gatgacgcca tgcgtaaagc aggctggatg agtgcgttga aagagcagga tattattccg
     8581 ccggaaagct ggattggcac tggtacgccg gacatgccgg gcggtgaggc ggcgatggtt
     8641 gaactgctgg ggcgcaatct acaacttacc gctgtatttg cttataacga caatatggcc
     8701 gctggcgcac tgacagcatt aaaagataat ggcattgcga ttccgttaca tctctcaatc
     8761 atcggtttcg atgatattcc catcgcccgt tacaccgacc cgcaattaac gaccgtgcgt
     8821 tatcccattg cttcaatggc taaattagcc accgaactgg ccttgcaggg ggcagcaggc
     8881 aatattgatc ctcgtgccag ccactgtttt atgccgacgt tagtgcgtcg tcattctgtc
     8941 gcaacgcgcc agaatgcggc ggcgatcact aactcaacaa atcaggcgat gtaaccgctt
     9001 tcaatctgtg agtgatttca cagtatctta acaatgtgat agctatgatt gcaccgtttt
     9061 aacgttgtaa cccgtatgta acagtgaata atcactttca ccgaggtaat agcgtcataa
     9121 caacaattaa agccgttttc tggagcgtta ccgggcatgg aagaacgaat tttaaaaagt
     9181 gagcttcggc gttcagtaac acttcattaa ctctactgcc ccgccgagca tttatctcaa
     9241 gcactaccct gcataagaaa aaccggagat accatgaata agaaggtgtt aaccctgtct
     9301 gctgtgatgg ccagcatgtt attcggtgcc gctgcacacg ctgctgatac tcgcattggt
     9361 gtaacaatct ataagtacga cgataacttt atgtctgtag tgcgcaaggc tattgagcaa
     9421 gatgcgaaag ccgcgccaga tgttcagctg ctgatgaatg attctcagaa tgaccagtcc
     9481 aagcagaacg atcagatcga cgtattgctg gcgaaagggg tgaaggcact ggcaatcaac
     9541 ctggttgacc cggcagctgc gggtacggtg attgagaaag cgcgtgggca aaacgtgccg
     9601 gtggttttct tcaacaaaga accgtctcgt aaggcgctgg atagctacga caaagcctac
     9661 tacgttggca ctgattccaa agagtccggc attattcagg gcgatttgat tgctaaacac
     9721 tgggcggcga atcagggttg ggatctgaac aaagacggtc agattcaatt cgtattgctg
     9781 aaaggtgaac cgggccatcc ggatgcagaa gcacgtacca cttacgtgat taaagagctg
     9841 aacgacaaag gcattaaaac tgaacagtta cagttagata ccgctatgtg ggataccgct
     9901 caggcgaaag ataagatgga cgcctggctg tctggcccga acgccaacaa aatcgaagtg
     9961 gttatcgcca acaacgatgc gatggcaatg ggcgcggtag aagcactgaa agcacacaac
    10021 aagtccagca ttccggtgtt tggcgtcgat gcgctgccag aagcgctggc gctggtgaaa
    10081 tccggtgcac tggcgggcac agtactgaac gatgctaaca accaggcgaa agcgaccttt
    10141 gatctggcga aaaacctggc cgatggcaaa ggtgcggctg atggcaccaa ctggaaaatc
    10201 gacaacaaag tggtccgcgt accttatgtt ggcgtagata aagacaacct ggctgaattc
    10261 agcaagaaat aagactgatt gggcgcagtc gtttactgcg cccttattgt atggccaaca
    10321 aggtaaaatt atatggtcag ctcaacgact ccgtcttccg gggaatactt gttggaaatg
    10381 agcggtatca acaagtcctt tcctggtgtt aaggcacttg ataacgttaa tttaaaagtc
    10441 cggccacatt ctatccatgc attaatgggg gaaaacggtg caggaaaatc gacattatta
    10501 aaatgcctgt ttggtattta tcaaaaagac tccggcacca ttttattcca gggtaaagag
    10561 atcgatttcc attccgccaa agaggcactg gaaaatggta tttcgatggt acaccaggag
    10621 ttaaacctgg tattacaacg ttcggtgatg gataacatgt ggctggggcg atatcccacc
    10681 aaaggcatgt ttgtcgatca ggacaaaatg taccgcgaaa ccaaagcgat ttttgatgaa
    10741 ctggatattg atatcgatcc gcgtgcgcgc gtcggcacat tatccgtttc gcaaatgcag
    10801 atgatcgaaa tcgccaaagc gttttcctat aacgcgaaaa ttgtgattat ggatgaaccg
    10861 acttcttcgt taaccgaaaa agaggtcaat catctgttca ctattattcg taaattaaaa
    10921 gagcgcggct gcggtattgt ttatatctcg cataaaatgg aagaaatctt ccagttatgt
    10981 gatgaagtta ctgtattgcg cgacggtcag tggatcgcca ccgaaccgct ggcaggactg
    11041 acgatggaca agatcatcgc catgatggtt gggcgttctc ttaatcagcg ttttcctgac
    11101 aaagaaaaca agccgggcga agtcatcctc gaggtgcgta acctaacgtc actgcgccag
    11161 ccgtcgattc gcgatgtctc gtttgatctg cataaagggg agatcctcgg tattgccggg
    11221 ctggtggggg cgaaacgtac cgatattgtt gagacgttat ttggtattcg cgagaaatcg
    11281 gctggcacca ttacgttgca cggcaaacag atcaataacc ataatgccaa cgaagccata
    11341 aaccacggat ttgcactggt aactgaggag cgccgctcaa cgggaattta tgcctatctg
    11401 gatattggtt ttaactcgtt aatttccaat attcgcaact acaaaaataa agttggttta
    11461 ctggataact cgcggatgaa aagcgatacc cagtgggtga ttgattcgat gcgggtaaaa
    11521 acgccgggtc atcggacgca aattggttcg ctctccggtg gtaatcagca aaaggtgatt
    11581 attggtcgct ggctactaac gcaaccagaa atattaatgc tcgatgaacc gacgcgcggt
    11641 attgatgtcg gggcgaagtt tgaaatttat cagttaattg ccgaactggc gaagaaaggc
    11701 aaggggatta ttattatctc ctctgaaatg cctgagttgt tagggataac agaccgtatt
    11761 ctggtcatga gcaatggtct cgtttccgga attgtcgata caaaaacaac aacgcaaaac
    11821 gaaattctgc gtcttgcgtc tttgcacctt taagattagg ggcttcccat gagtgcgtta
    11881 aataagaaaa gttttcttac ttacctgaaa gagggcggta tttacgtcgt tcttttagtt
    11941 ttgctggcga ttattatttt ccaggaccca acatttttaa gtctgttgaa cttaagtaat
    12001 attctcaccc agtcatcggt gcgtattatt atcgcgctcg gtgtggcagg gttaattgtc
    12061 acccagggga ccgatctttc tgctggtcgt caggtagggc tggcggcagt ggtggctgcg
    12121 acattattgc agtccatgga taacgccaac aaagtgttcc cggaaatggc gacgatgccg
    12181 attgcgctgg ttattctgat tgtctgtgcc attggtgcgg tgatcggttt gatcaacggt
    12241 ctgattatcg cttatctcaa cgtgacgccg ttcattacca cgctcggcac gatgatcatc
    12301 gtctatggca tcaactcgct ctattacgac tttgtcgggg cgtcgccaat ttctggtttt
    12361 gacagtggct tctctacctt tgctcagggc tttgtcgcgc tggggagttt ccgtctctct
    12421 tacatcacct tctacgcgtt gattgcggtg gcgttcgtct gggtgttgtg gaacaaaacc
    12481 cgcttcggta agaacatttt tgccattggc ggtaacccgg aagcggcaaa agtatctggt
    12541 gtcaacgtcg gcctgaacct gctgatgatc tacgcgttgt ctggcgtgtt ctatgccttt
    12601 ggcgggatgt tagaagccgg acgtatcggc tctgccacca acaacctcgg ctttatgtat
    12661 gagctggatg ctatcgcggc gtgcgtggta ggcggtgtat cgttcagcgg cggtgtgggg
    12721 acggtgattg gcgtggtgac cggggtaatt atttttaccg tcatcaacta tggcctgacg
    12781 tatatcggcg taaacccata ctggcagtac atcatcaaag gggcgattat tatcttcgcc
    12841 gtagcgctgg attcactgaa atacgcgcgt aagaagtgat gtgattgtgg atattgccgg
    12901 atgcgctgca tccggcaatc ggtgccaaat gccggatgcg tctctggttt tacaacgtta
    12961 ccgggtgttc tttctcgcct ttcttaaact tcacttcccc gagctcaata caacccaccg
    13021 ggcagacgtg accacaaagc agacaaccca cacatttctc ggtattacaa tgcggtgtgc
    13081 gggttttctc gctccattcc atcgcctggt gaccgccgtc gtaacaggaa atataacagc
    13141 gtccacagcc aacacattta tcaagattga tacggggata gacaatataa ctgcggtcta
    13201 aatcttccgc cgggacaata ttgttattcg ccagacctac catctcctgc agcgaatcaa
    13261 atccctgatc ggcgagataa tgcgacaacc cgcttgccat atcttccact atccgatacc
    13321 cgtactgcat gatgccggtg gtcacctgta acgttgctgc gccgagcaat aaaaactcag
    13381 ccgcatcctc ccaggtttca atgccgccga taccgatgat tgggaaatcg cgcagttctg
    13441 gatgggtgcg catttgctga atgaagcgca gggcgatcgg ttttaccgct ttaccggaat
    13501 atccggaaat actcgatttt ccgttaacga tcggcatacc gattttctga ttaagatcga
    13561 tattggtgat ggatttaacg gtgttaatcg ccgcaatgcc atctgcgccg ccgcgcttcg
    13621 ccgccagcgc cacttcgcac atatcaccga tattcggcgt catcttcgcc agcattggca
    13681 gcgtggaacc ccgtttcact gcccgacaat atttttctac cagctccggg ctttgcccga
    13741 catcgctacc catcgcatga gaagtcattt gcggacagga gaagttacac tcgatcatat
    13801 ccgcgccagc ttcttgcacc aggcgcgcca gctcctccca ttgctgctca ttttccccca
    13861 tgatcgaagc gatcaatact ttgtccgggt aatcttcctt cagctgacgc agggcggcca
    13921 gattctcttc caacggatgt tcagcaatct gctccatatt tttgaagccg ataaaaccgg
    13981 tatcttcttt caccagatga tcaaaacgcg gcgagacttc gttggcgata aaaaagccga
    14041 tcgttttaaa caccacgccg ccccaacctg tgtcgtaggc tttggcacac atctcatagc
    14101 agttgcctac aggcgaagaa gagaggcaga acgggttggg aaacttcacg ccgcaaaaag
    14161 taatcgaaag atctttcgtt aacatgagca agctccctct aaatagtgat gaatcgcctc
    14221 ggcggcttct ttcccggttt tcacggcata gaccacggtt ttgtcaccct caacaatatc
    14281 gccagcagca aagacttgcg gatcgcgggt ctgataattt tgtgttttga tggtgttacg
    14341 ctgcggctct aactccgcaa aggcatccag tctggcatgc tgaccgacgg cgagaataat
    14401 tttatctgcc gccatcgtca gttcgcccgg taaccgtaca tgcttaaacg tgactttatt
    14461 gccttccacg gctactggcg tgaatccatc gatgatcgaa acgcccagct cccgggcgct
    14521 ggcaaactct ttttcgcttg ccggaaactc atctaactct tcacgcgcta cgcaggttac
    14581 cgcctgacag ccgagaactt tcagcgtgct ggctacgtcc atcgcaacat caccgccgcc
    14641 gataattaat gcgctttgtg gaatgctgat atcgccttgc gcctgtcgtg cacgttgcag
    14701 gaagtccacg gcaatctcaa cgttgctatg ctcaaacagc gataacccgg aaccgctcga
    14761 taaccccacg gtgaccagta ccgcgcggtt ttctgctttc agctgctcaa gggtgagtgt
    14821 gttaccgact tcgttgttgc acttaatggt cacgcccatt ttttcaatac gggcgatctc
    14881 tgcatccagc actgattgcg gtaaccggaa ttgcggaata ccgttacgca accagccacc
    14941 ggggtgcgct tctttctcat aaatcgtgac gtcataaccc tggtttgtca gtgttacact
    15001 ggcctgcaat ccggcaggac ctgcgccaat aatcgcgact ttgccgagca ttttagtacc
    15061 gggctgataa atttccatcc cagtttgttg ttcaaaatca gtaacaaaac gctgtaagcg
    15121 accgatatca atgggagtat caacaccggc acgggtacaa ccgctttgac ataatttctc
    15181 cgtcgggcac actctggcac aaacggcacc gagggcatta ttttcgcgaa ttgtctcggc
    15241 agcgccttta aaattacgaa agtagattga gcgaataaat ttccccggat cggtctgtgc
    15301 cgggcaagcc tgactacagg gagcgtcatg acataataaa cagcgagagg cttctttaat
    15361 cgccagtaaa ggcgtaaaag ccggagtgag ttcatccaga taattttgtt gcggcatgtg
    15421 atcttcctta atgctatgag taattaaggc gaacgctaaa tatactcgtc atacttcaag
    15481 ttgcatgtgc tgcgtctgcg ttcgctcacc ccagtcactt acttatgtaa gctcctgggg
    15541 actcactcac ttgtcgcctt cctgcaactc gaattattta gagtataaat attgcgtgct
    15601 gatattaagg ctatgcgcgt ggggaaattt atactgtgaa aggattcaca aatagctgcg
    15661 gttcagaatg ttatatccgc agcttattga gtgaagtaat aattattctc aataattaac
    15721 agaaaagaga aaatattaac gatagaaaat aagataaaca atcatgccga tagataaaat
    15781 tccgaagccg atatatgcca ccatataagc gaaaaagagt ccgggatttt ttgctcgcca
    15841 gacatacacc ggttccggtg catttttaac ccgtttatca atcgcgcctg cgcgccagcc
    15901 tttccatgct tcgcggaaac aaaatatcgg aataaggaaa aaaacggcaa cgacaaaaaa
    15961 ctgctgtaca tccataacta ctttccttgt ttcttttgta attcaagtaa ctgttcgggt
    16021 gtgacggcgg gatacccctg cgcatcttcc ggcacctggt gcatagccgg aatagggacc
    16081 agcggcccta aaaaacgcgg ttcacgttta aaaatataaa ggtcagccag cgcaccgaaa
    16141 cgggcggcaa attcgcgaat acgtactgac agcatatctt tcggtgacgg tacggcataa
    16201 cactgagcct gaatccccat atgcagcgca ataaataatg cccgctcaca gtggaaacgt
    16261 tgggtgataa taatgaaatc attagtatcg aaaactttgc gtgtacgcac gatggaatcc
    16321 agcgtacgaa agcctgcgta atcgagaaca atatctgatg ggtcgacacc agcagcgatt
    16381 aaatctttgc gcatggtcat cggctcatta taactttgca atgcgttatc gccgctcagt
    16441 aatagataat ttaccttacc gctgttatag gcattaatcg ctccttgaat gcggtagcga
    16501 taatactgat taattacgcc agtacgataa tattttgctg ttccgagcac cacaccgacc
    16561 tggcggtagg ggagatcctg caattcgtcg tagatataag gcgcggtttt ccagctcatc
    16621 cagcgatcga ggccgagcac agtcaacagc agcaagccga tcaggactaa caggctgagg
    16681 aacacgcgct ttaacatgca ggagactcga tctaaggaaa atttccatca ggctacttta
    16741 cccgctggtt ttgcgcaaga aacggcaaac tgtttgccgg gaatttcgac accaggagag
    16801 ggctggcaag ccggacaagg cgttcacgcc gcatccggca ccaggcttaa gcgagaagca
    16861 ctcggtcgat actgtgacag ccgagagctt tcagtgttgc ggaggtggca tcccactgaa
    16921 tcaacggcgc atcggctttt tctgccagaa ccgcgcgctg gatatccggg taatcataac
    16981 ccttgagatt caatagaatt aacgctccct gcaacggtgg cagagtcggg ttgaatgcgg
    17041 cgttttcagc gtagctgccg ctgaaaatac gaccgtcttt acattccagc gcgacaccgc
    17101 ttggcgactt actgtaaggc atgtgcgaac ggtttgccgc tgcaatcgcc gcctgagaaa
    17161 gcgcatcacc agtcagcgca tagccgtgat cctgctcgtc catcagcagc gttttaatct
    17221 ccagatcttt cggcccaaag gcatccggca gatagtcacg cagcgcgtgt gcctcgcggc
    17281 ccggcagatg aatacgcaga tccagaccgc tgttcagttc attcataaac tgacggcagt
    17341 gaccacaagg cgtgtagtta acggtgatgg ctgcaagcgc tttttcacca ctcaaccagg
    17401 cgtggctgat cgcgctttgt tcggcatgaa cggtttgctg cattgtcgca ccaataaatt
    17461 ccatattggc accgaaatac caggttccgc tcacaccgcg cgcaattgcg ccaacattaa
    17521 aattcgacaa tggcgtacgc gcacaggccg ccgccagcgg aagtagtgcg aatgccagcg
    17581 cgtcttcgtc cagccccgtt gcgctcttca gcgatgagac ttgctccccg gtcaacaaag
    17641 cggggaagta cttgtctgcc agaataggtt ccagtgcaga ttgcaagtta tccgcaagtt
    17701 gggcaaaagc ggtttgaaaa cgtggatgca tggcgttgcc tcataatcat gtaatgggat
    17761 aacagtttac gaacccgtta aggctttata tgtgatctga atctcattaa ttatgcaacg
    17821 cgtgaaactt acatcaaaaa tgcgcgacgc atcgcaaatt ttagcccatt actgccagaa
    17881 taatcgggaa aaggaacggt gcgatcagcg aagtaattat cccgcataac accagcgcta
    17941 gcgaactaaa tgcaccttcc tgataatcca gctcggcgca gcgcgccgta ccgagggcgt
    18001 gcgaggcagt ccccattgcc agaccgcgcg cagctttggt acgaatacgc atcgcattaa
    18061 gcaatgtatg gccaaatacc gcgccgagga tgccgacgaa aatcacgcaa actgcgctga
    18121 ttgccggaat accgccaata ctgccgccaa ccgccattgc aataggcgtg gtgactgatt
    18181 tcggcaggat tgacgcggcg atttccggtg aagcgcccat caacaatgcc acggaagtcc
    18241 ccgtcaccat cgcaaccacg ctgccgataa aacagatggt gatgatcgat ttccagcgcg
    18301 cgcggatctg gtgtagctgc tcatataaag gataggccag cgcgacgacc gccggttgca
    18361 gcagatcgtt tagcacttcg ctacctttaa agtagctgtc gtaagagatg ccggtcagca
    18421 ttaaaaaagg aatgatcacc accattgcta ccagcaacgg gttaagcagg ggaaacttat
    18481 accgtgccgc cagtttgcgg gcggcaaaaa agacaatcaa cgtcagcggt aatgaccacc
    18541 agatattcgc catcattctt ctgatccttt ctgacctacg actttacgtt caccgtgtac
    18601 cagttgtgaa ctccagctca ccaccagaaa aaccaccagc gtactgattg cacaggaaac
    18661 cactaccggg ccaaactgtg cgcggagcaa atcaaaatat tgcatgacgc ctacgccaat
    18721 cggcacaaac aatagcgcca tatagcgaat cagtacgtag caccccggat tgacccattt
    18781 tgccggaaga atttgcaagg ccagcaggac aaacaggatc agcatcccga tgatgctgcc
    18841 cggaatggtt accggtagca gggaagcaat gaaaatgcct gcatacaggc aggcataaat
    18901 caggacgaaa gcgcgtaaat attgccagat aatgttcagt gtcttactca taatcaacac
    18961 cccagatgag atggattcat catacaatta attcgtaaaa cgtgctaccg atcacattaa
    19021 gaaatcagcg cttatttttt cgctccccgg cattacgcaa tgcgcaacga actcaggcat
    19081 acatcaagca tagagaggga ttgcagtttg cagcaatccc tctttgtaga cctttaatcc
    19141 gctgaccata ggcgctaaca tagcgcctca ttattttgcg ggtgatgata tgcgtgtgtt
    19201 actggcaccg atggagggag tgcttgactc tctggtgcgt gaattgctga ccgaagttaa
    19261 cgactacgat ctgtgcatca ccgagtttgt ccgcgtggtg gatcaactgc tgccggtaaa
    19321 agtctttcat cgcatttgcc ctgagctaca aaacgccagc cggacaccat ccggtacgct
    19381 ggtgcgcgtt cagttgttag gtcagttccc acaatggctg gcagagaacg ccgcccgtgc
    19441 ggtggaatta ggttcctggg gcgtggatct caattgcggc tgcccgtcga aaacggttaa
    19501 cggcagcggc ggcggggcga cgttactcaa agatcctgaa ctcatctacc agggtgcaaa
    19561 agcgatgcgt gaagctgtac cagcgcattt gcccgtcagc gtaaaagtgc gtctgggctg
    19621 ggacagcggt gagaagaaat ttgaaatcgc cgatgcggtt caacaggctg gcgctacgga
    19681 gctggtggtg catgggcgga caaaagagca gggttaccgc gcggagcata ttgactggca
    19741 ggcgattggc gagattcgcc agcggctgaa tattccggtg attgccaacg gtgaaatctg
    19801 ggactggcag agtgcgcaac aatgcatggc gatcagcggc tgcgacgcag tgatgattgg
    19861 tcgcggggcg ctcaatattc ccaacctgag ccgggtagta aaatataacg aaccgcgaat
    19921 gccgtggccg gaggtggttg ctttgctgca aaaatatacc cgtctggaaa agcagggcga
    19981 taccgggtta tatcacgttg cgcggattaa acagtggttg agttatttgc gtaaagaata
    20041 cgatgaagca acgggattat ttcagcatgt tcgggtgttg aataattccc ctgatattgc
    20101 aagggctatt caggcaattg atatcgagaa actctaaaaa accatagtga tgttttttga
    20161 caatgtgtta gctctttatc aataggccgc acggttgttt ggtggcgtca aaattgccga
    20221 ggcgttccct gaaggccggg gcagcccaca tggatgtggg ctgagggcgc gttttacagg
    20281 gatgttacct cgcgcccgac ccggtagccg taagggataa gccgagggca ccgcgcagcg
    20341 gcgattttgt tcgccagagc ccgggggtgc agggggcggc ggcgattggc cgccccctgc
    20401 gcgctccttg cgccagtgat tatatgaagc aatgcacatg acaggagcgc aacaggatga
    20461 gagactgaaa tattctcgtt attttcggac atgatgacat taattaattt taaaatatca
    20521 tcttaaatac gccagtcacc accagtagcc caatcaggaa aataatcaat acagcccaga
    20581 gtataatttt cattggcgtt cctttatttg tgagtgctga ataagtatag gaaaaaacga
    20641 agccctttgc cgttatccat aaacgattaa gataaatcgg aagatatcat tcacttagtg
    20701 tatatttttc tggatatgtc gttttattgc tactgatgta ttgccacgcg aaactaaaaa
    20761 atatatcgca acacacggta atctccgcgc accttttctt acctgattat tcccccatga
    20821 atcgtgattc cttttatccc gccatcgcct gttttccgct gttactgatg ctggccgggt
    20881 gtgcgcctat gcatgaaacc cgccaggcat taagccagca aacgcccgct gcacaagttg
    20941 acaccgcatt acccacggcg ctgaaaaatg gttggccaga cagccaatgg tggctggagt
    21001 atcacgataa tcaactcact tccttaatta acaatgcact acagaacgca ccggatatgc
    21061 aggtggctga acagcgcatt cagctagctg aagcccaggc gaaggcaatt gctactcagg
    21121 atggtccgca gatcgacttt tctgcggata tggagcggca aaaaatgtcg gcagaaggct
    21181 taatggggcc gtttgctctg aacgatccgg ccgcaggtac gaccggtccg tggtacacca
    21241 acggaacttt tggcttaacg gcgggctggc atctcgatat ctggggaaag aatcgggcgg
    21301 aggttactgc ccgcctgggt acggttaaag cacgggcggc ggaacgcgag caaacccgcc
    21361 aattgctggc tggcagcgta gcccgcctgt actgggagtg gcaaacccag gcggcgttaa
    21421 acacggtctt gcagcaaata gaaaaagagc agaacaccat tatcgcgacc gatcgccagc
    21481 tatatcagaa cgggattact tcttcagttg aaggtgtgga aaccgatatt aatgccagca
    21541 aaacccggca gcagctcaac gatgtcgcgg ggaaaatgaa aattattgag gcacggttaa
    21601 gcgcacttac aaataaccag acaaagtcat tgaagcttaa accggtcgcg ttgccgaaag
    21661 tggcaagcca gcttcctgat gaactggggt actccttact ggcccggcgg gcagatttgc
    21721 aggcggcgca ctggtacgtt gagtcatcgc taagcaccat tgatgcggca aaagcggcat
    21781 tttatcctga catcaacctg atggccttcc tgcaacagga tgcgttgcac ttaagcgatc
    21841 tgttccgtca ttccgcgcag caaatgggcg ttacggcagg cctgacgcta cccattttcg
    21901 atagtggtcg tcttaacgcc aatctcgata tcgcaaaagc cgaaagcaac ttgtctatcg
    21961 ccagctacaa caaagcggtg gttgaagcgg tgaatgacgt ggcgcgggca gccagtcagg
    22021 ttcagacact tgcggagaaa aaccagcatc aggcgcaaat tgagcgcgat gccttgcgtg
    22081 tggtaggtct tgcgcaggcg cgctttaacg cgggcatcat tgctggttcc cgcgtcagcg
    22141 aagccagaat ccccgcgctg cgtgagcggg ccaatggcct gttattgcaa gggcagtggc
    22201 tggatgcttc cattcaactc actggtgcgt tgggcggggg ttacaaacgc tgatgatatg
    22261 cgccttctat acttaacgtt tattcagcgt taagtggaga actcgatggc acaggttgcg
    22321 attattaccg cctccgattc ggggatcggc aaagagtgcg cgttattact ggcgcagcag
    22381 gggtttgata ttggcattac ctggcactca gatgaagaag gggcaaaaga taccgcgcgt
    22441 gaggtagtta gccacggcgt acgtgcggag attgtgcagc tggatctcgg taaactgcca
    22501 gaaggtgcac aggcgctgga gaaactcatt caacggctgg ggcgtattga tgtgctggtg
    22561 aataatgcgg gcgcaatgac caaagcgccg tttcttgata tggctttcga tgagtggcgc
    22621 aagattttta ccgttgatgt cgatggtgca tttttatgct cacaaattgc ggcccgccag
    22681 atggtgaaac aagggcaggg tggtcgcatc atcaacatta cgtcggtaca tgaacatacg
    22741 ccgctgccgg atgccagcgc ctacacagcc gctaaacatg cgctcggcgg gttaaccaaa
    22801 gcaatggcgc tggagttggt caggcataag attttggtga acgcagtcgc acctggggcg
    22861 atcgctacgc caatgaacgg catggatgat agcgacgtga agcccgacgc ggagccttcg
    22921 attcctctgc ggcgttttgg cgcaacgtat gagattgcca gcctggtggc gtggctttgt
    22981 tcggagggcg caaattacac caccgggcag tcgttgatag tggatggcgg ctttatgttg
    23041 gcgaatccgc agttcaaccc agaatagcgc agtgaggtgt gtggatattt caatgggtaa
    23101 aattttggta ggcatgataa gacgcacaag cgccgcatca ggcatggtgc accggatgtc
    23161 ggatgcggcg cgaacgcctt atccagggca atggttttac gcctgattat ccggcttttt
    23221 cttcccgcgt cgtttcagcc accagcgcac gcctaccacc agaaccacga ccagaatcaa
    23281 ccagacccag tgcttcaaat gctgatcgag attgtgcaac cacggcgcaa tcacctgacc
    23341 accagcgtaa ccgatagtgg taaaaatcaa cgcccaggca aatgcgccga gaatattaag
    23401 cggcagaaag attttcggcg gcagctggct ggcaccaatc agcgtcgggc caatcacccg
    23461 aaagccatac ataaagcgcg taccaatgac aaacagatac ggatggcgtt ggataagttt
    23521 ctgcgcccgc tcaattttat cctgatgttt cgagaaacgg cgtaacagct tgccgccaaa
    23581 ccgccgcccg cacagataaa gcacctggtc gccaatcatg ccgccaagcg ccacagaaag
    23641 taccaccagc gggaacttta atagcccctg atgcgccgca acgcctccca gcaaagtcac
    23701 ggtttcacct tccgccaggc taccgatcac cagcgcggca taaccatatt gtgagatgag
    23761 tgtattgaga tccataggcc agtctgtctc cctttggttg cctgtaagca tacaccctgg
    23821 aaatcattca cgctgaaaaa ggacgtacat cagacggtgc aaattacatg agcgaacaaa
    23881 aaataatcac aagctgactt atacttgaac tgtcaggttc atgaactcac aacaaggggg
    23941 cggtatgagc catgtctggg gactgttttc ccatcccgat cgtgaaatgc aggtgattaa
    24001 tcgcgaaaac gaaacgattt ctcaccatta cacccaccac gttttgctga tggcggcgat
    24061 tccggtgatt tgcgccttca ttggcactac acagattggc tggaattttg gcgatggcac
    24121 tatcctgaag ttatcctggt ttaccggact ggcgctggct gtcctgtttt atggcgttat
    24181 gctggctggg gttgcggtaa tggggcgggt catctggtgg atggcgcgta attatccgca
    24241 gcgtccgtca cttgcgcact gcatggtctt cgcgggctac gttgcaactc cgctgttttt
    24301 aagcggtctg gtggcgcttt atccgctggt ctggttgtgt gcgctggtcg gcacggtggc
    24361 gctgttttac accggctacc tgctttacct tggtattccg agcttcctga atatcaacaa
    24421 agaggaaggc cttagcttct ctagttcgac gctggcgatt ggcgtgctgg tactggaagt
    24481 gctgctcgcc ctaaccgtta tactatgggg ctacggttat cgtttgttct agaaagttct
    24541 ttgacgttgc attgctggcg taaatgtcag caatgcagca tttcctcacg attctccttt
    24601 gacgatcgca ctttttgctc gttatgatgg cgtcgctagc ctcagtaaat ccttactccg
    24661 gcggtgcgca acccgtgcgc gtgaaccact atctgaatgc tcatcatgcc gaaatttcga
    24721 gtttctttat ttagcctggc cctgatgctg gctgtgcctt tggcaccgca ggccgttgcg
    24781 aaaacggcag ccgctaccac cgcttcacaa ccggaaattg cctccggtag cgcgatgatt
    24841 gttgatctga ataccaacaa agtgatctat tcgaaccacc cggatctggt gcgtccgatt
    24901 gcgtctatca gcaaattaat gaccgcgatg gttgtgctgg atgcacgact gccgctggat
    24961 gaaaaactaa aagtggatat cagccagacg ccggagatga aaggggtcta ttcgcgcgta
    25021 cgactgaata gcgaaatcag ccgtaaagat atgctgttgc tggcgctgat gtcttcagaa
    25081 aaccgcgcgg cggcaagcct tgcgcaccat tatcccggtg gttacaaagc ctttattaag
    25141 gcaatgaatg cgaaagcgaa atcgctcgga atgaacaaca cgcgctttgt tgaaactacc
    25201 ggattgtcgg tgcataacgt ttcaactgcc cgtgacttaa ccaaactgct cattgccagc
    25261 aaacaatatc cgttgatcgg gcagttaagt actacccggg aagatatggc aaccttctct
    25321 aacccgacgt atacgctgcc gttccgcaat actaatcatc tggtgtatcg cgataactgg
    25381 aatattcagt taaccaaaac cggctttacc aatgcggcgg gccattgtct ggtgatgcgt
    25441 acggttatca ataataaacc ggtggcgctg gtagtgatgg acgcgtttgg caaatatacc
    25501 cattttgccg atgccagccg cctgcgtacc tggattgaaa ccggtaaagt gatgcctgtg
    25561 ccggcagcag cgttgagcta taaaaaacaa aaagccgccc aaatggcggc ggggcagacg
    25621 gcacagaacg attaagtgaa ttcggatggc gatactctgc catccgtaat ttttactcca
    25681 cttcctgcca gtttttccgt ttactggttt taccgatccc cggattcatg ctgttggtcg
    25741 gatcgttctc gcgatagaac ttctgcaacg tctccggtgc tttatacaaa tgaccgacgt
    25801 tatgctcggc agggtactgc gcgccgcgct gctgtagcag ttccagcatc tgttctttta
    25861 acgcatgcac atccacgcct tttttcacta tgtaatcctg atggaagaca tagcacataa
    25921 aatggccgta atagagcttg tgcaccagct ggctgtcgat ctccggcggt aaatgctcat
    25981 accactcggt gtcgttacgc cgcagagcga tatccaacgc cagaatgtct tcgacttcat
    26041 cggaatgcac cgcctgataa cgaattgctg cgcccgcagc ggcgaaacgg tgtaaaaacg
    26101 ctttgctgcc ttcctccggc gtacagacaa agaaatcgcc ttcggcctgt ttgaaataat
    26161 ctaccagcca cgatttggct tcaccgacgc catcgcccgc catttttaac agcagatgat
    26221 gctcgtattt atcgcgccag tttttcatgc gcggcggtaa atggctgggg aacaggtgac
    26281 cgaatttttg catcgcacgg tcagtaaaat gcggacggaa gaatttcact ttctccagca
    26341 tcgcatcggt gcgtcccttg agattaaaga agaacggcat cttgtcggtg cctaacttat
    26401 caatcatcag gaaggtgtct ttgccgtatt tttccgcaat atcgtagata tcccggtgca
    26461 tatattcccc ggcaaccggc agattttcga agttagccag aatatgacgg cggatttcgg
    26521 tcagcacttc cggctggttg gtgccaatat aaaacacctg ctgatttttt tccgcctcga
    26581 aggtatcaag acgtactgca aagacggcca gcttcccggc gcaaccagaa gattcaaata
    26641 accgatccgg atcggcgtta taacgtgcgg gcgtgtcggc ttcaatatca cgaacgcggt
    26701 ggacatagtc ataatcgtgg gcgtgacgac catcgtgacg cacatcgtca tctttgatgc
    26761 gatcatcatc cagcttgcta aggatctgct ccggcgtttc gcccagatca atccccagat
    26821 ggttcaccag cgtcagtttg ccgtcttcat ttatacgcgc gaataacgac atttcggtat
    26881 acgccgggcc acgttgcacc agcgagccgc cggagttgtt acaaatacca ccgatgaccg
    26941 atgcgcctat acacgatgat ccaatcactg agtgcggttc gcgtcccagc ggtttgaggg
    27001 ctttttccag cgaatagagc gtggtgcccg gataggccag cacctgttcg cccttgccga
    27061 gaacgtgcag cttgtcgaga cgcagggtgc tgatgatcac gatatcgcga tcatagtcgt
    27121 taccgtttgg cgtcgatcct tcggtcaggc ctgtattggc agcctgcatc agaataattt
    27181 tgtcggcggt gacgcaggct ttcagcaccc gccacaattc cagtagtgag ccagggaaaa
    27241 cgacagccag cgcgtcgccc tgaccagaac ggaagccctt gcgatagcgg gccgtttttg
    27301 cgggatcggt gagcaggtgt gaatgaccga ccagacgggc aagttcattc aaaaaggctt
    27361 tattatcagt tgttgtcatg gaagacattt tccactcctt gtggtggcga aaaaatatca
    27421 caaactagaa tagcgccttg tatgacaatt cagcgagtga atattttgaa aattcagaca
    27481 ataaactgag cattcttttt ctctctccat catgcttatg gcaaactacg gtttttgcac
    27541 cattttcgct gcctcgtcgg gcaacagagg aagaaaaatc catatgaaat ggctatgttc
    27601 agtaggaatc gcggtgagtc tggccctgca gccagcactg gcggatgatt tattcggcaa
    27661 ccatccatta acgcccgaag cgcgggatgc gttcgtcacc gaactgctta agaaaatgac
    27721 agttgatgag aaaattggtc agctgcgctt aatcagcgtc ggcccggata acccgaaaga
    27781 ggcgatccgc gagatgatca aagacggtca ggttggggcg attttcaaca ccgtaacccg
    27841 tcaggatatc cgcgccatgc aggatcaggt gatggaatta agccgcctga aaattcctct
    27901 tttctttgct tacgacgtgc tgcacggtca gcgcacggtg ttcccgatta gcctcggtct
    27961 ggcctcgtct tttaacctcg atgcagtgaa aacggtcgga cgtgtctctg cttatgaagc
    28021 ggcagatgat ggcctgaata tgacctgggc accgatggtc gatgtctcgc gcgatccgcg
    28081 ctggggacgt gcttccgaag gttttggcga agatacgtat ctcacctcaa caatgggtaa
    28141 aaccatggtg gaagcgatgc agggtaaaag cccggcagat cgctactcgg tgatgaccag
    28201 cgtcaaacac tttgccgcat acggcgcggt agaaggcggt aaagagtaca acaccgtcga
    28261 tatgagtccg cagcgcctgt ttaatgatta tatgccgccg tacaaagcgg ggctggacgc
    28321 aggcagcggc gcggtgatgg tggcgctgaa ctcgctgaac ggcacgccag ccacctccga
    28381 ttcttggctg ctgaaagatg ttctgcgcga ccagtggggc tttaaaggca tcaccgtttc
    28441 cgatcacggt gcaatcaaag agctgattaa acatggcacg gcggcagacc cggaagatgc
    28501 ggtgcgcgtg gcgctgaaat ccggaatcaa catgagtatg agcgacgagt attactcgaa
    28561 gtatctgcct gggttgatca aatccggcaa agtgacgatg gaagagctgg acgatgctgc
    28621 ccgccatgta ctgaacgtta aatatgatat gggattgttt aacgacccat acagccattt
    28681 ggggccgaaa gagtctgacc cggtggatac caatgccgaa agccgcctgc accgtaaaga
    28741 agcgcgtgaa gtggcgcgcg aaagcctggt gttgctgaaa aaccgtctcg aaacgttacc
    28801 gctgaaaaaa tcggccacca ttgcggtggt tgggccactg gcggacagta aacgtgacgt
    28861 gatgggcagc tggtccgcag ccggtgttgc cgatcaatcc gtgaccgtac tgaccgggat
    28921 caaaaatgcc gtcggtgaaa acggtaaagt gctgtatgcc aaaggggcga acgttaccag
    28981 tgacaaaggc attatcgatt tcctgaatca gtatgaagaa gcggtcaaag tcgatccgcg
    29041 ctcgccgcaa gagatgattg atgaagcggt gcagacggcg aaacaatctg atgtggtggt
    29101 ggctgtagtc ggtgaagcac aggggatggc gcacgaagcc tccagccgga ccgatatcac
    29161 tattccgcaa agccaacgtg acttgattgc ggcgctgaaa gccaccggta aaccgctggt
    29221 gctggtgctg atgaacgggc gtccgctggc gctggtgaaa gaagatcagc aggctgatgc
    29281 gattctggaa acctggtttg cggggactga aggcggtaat gcaattgccg atgtattgtt
    29341 tggcgattac aacccgtccg gcaagctgcc aatgtccttc ccgcgttctg tcgggcagat
    29401 cccggtgtac tacagccatc tgaataccgg tcgcccgtat aatgccgaca agccgaacaa
    29461 atacacttcg cgttattttg atgaagctaa cggggcgttg tatccgttcg gctatgggct
    29521 gagctacacc actttcaccg tctctgatgt gaaactttct gcgccgacca tgaagcgtga
    29581 cggcaaagtg actgccagcg tgcaggtgac gaacaccggt aagcgcgagg gggccacggt
    29641 agtgcagatg tacttgcagg atgtgacggc ttccatgagt cgccctgtga aacagctgaa
    29701 aggctttgag aaaatcaccc tgaaaccggg cgaaactcag actgtcagct tcccgatcga
    29761 tattgaggcg ctgaagttct ggaatcaaca gatgaaatat gacgccgagc ctggcaagtt
    29821 caatgtattt atcggcactg attccgcacg cgttaagaaa ggcgagtttg agttgctgta
    29881 atgctttagt aagggcgcga cgtttgcgcc ctttgtaggc cggataaggc gctcgcgccg
    29941 catccggcag gaaacgccgg accgaatatg cctcttaagt atttatctga ttctgcaact
    30001 gaatccttcc gctcaagcta accccgccat tatcaactat gcttttctct taattcgctg
    30061 aaaacaacag taaaagaatg aggaaagcat catgccactc ttaaagctct gggcaggttc
    30121 actggttatg ttggcagccg tgagcctgcc gctacaggcg gcttcccccg ttaaagtcgg
    30181 ttcaaaaatc gataccgaag gtgcgctact cggcaatatc attttgcagg tgctggaaag
    30241 ccacggcgta ccgacggtga ataaagtgca actcggaacg actcctgtgg tgcgcggggc
    30301 gattacttcc ggtgaactgg atatctatcc ggaatatacc ggcaatggcg ctttcttctt
    30361 taaagatgaa aacgatgcag cgtggaaaaa cgcgcagcaa ggttacgaga aagtcaaaaa
    30421 gctcgattcg gagcacaaca agttaatctg gctgacgccc gcgcctgcaa ataacacctg
    30481 gactatcgcc gtgcgtcagg atgtggcaga gaaaaataaa ctcacttcgc ttgccgacct
    30541 gagtcgttat ctgcaagagg gcggcacctt caaactggca gcctcggcag agtttatcga
    30601 acgcgccgat gcgttacccg cgtttgaaaa agcctatggc tttaagctcg gtcaggatca
    30661 gttgctgtca ctggctggtg gcgacacggc ggtgacgatc aaagccgctg cccagcaaac
    30721 ctctggcgtt aatgctgcaa tggcttacgg cactgacggc ccggtagcgg cgctggggct
    30781 gcaaacctta agcgatccgc aaggcgtgca acctatctac gcgcctgcac cagtggtgcg
    30841 tgagtcggtg ctgaaagagt atccgcaaat ggcacagtgg ctacagccag tcttcgccag
    30901 cctcgatgca aaaacattgc agcaactgaa tgccagcatt gcagtggaag gactggatgc
    30961 caaaaaagtg gctgccgact acctgaaaca aaaagggtgg acgaagtaat ttcccgtgac
    31021 ttatctacgt attaatcctg ttctggcgct gctgctgttg ctgacggcaa tcgcagcagc
    31081 gctgccgttt atcagctacg cgcctaatcg tttagtctcg ggtgaagggc gacatctctg
    31141 gcagctgtgg ccgcaaacga tctggatgct ggtgggcttt ggttgcgcct ggctgacagc
    31201 ctgttttatt cccgctaaaa aaggcagcat ttttgcactc attctggcgc aattcgtctt
    31261 cgtattgctg gtgtggggag ctggaaaagc ggcgacccaa ctggcgcaaa atggcagtgc
    31321 gctggcgcgt accagcctcg gcagtggttt ctggctggct gcggcgctgg cattgctggc
    31381 ctgtagcgat gccatccgcc gaatctccac gcatccgctg tggcgctggt tgttgcatat
    31441 gcagattgcc attattccgc tgtggttgct gtactccggc acgcttaacg atctctcgct
    31501 aatgaaagaa tacgccaacc gtcaggatgt gtttgacgac gcgctggcac aacatctgac
    31561 gttgctgttt ggtgcggtgc tgcctgcgtt agtgattggt gtgccgttgg gcatctggtg
    31621 ctacttttcc actgcgcggc agggggcaat tttttctctg ctcaatgtca ttcagaccgt
    31681 gccttcggtg gcgctctttg gcctgttgat tgcgccgctt gccgcgctgg tgacggcctt
    31741 tccgtggctg gggaagctcg gcatagcagg aaccggaatg acacccgcac tgattgcgct
    31801 ggtgctctat gccttgctgc cgctggtgcg cggcgtggta gtaggcttga accagatccc
    31861 gcgcgatgtg ctggagagcg ccagagcgat ggggatgagc ggggcgcagc gattcctgca
    31921 tgttcagtta ccgctggcgt taccggtatt tctgcgcagc ctgcgggtgg tgatggtgca
    31981 aactgtaggt atggcggtga ttgcggcgtt aatcggcgca ggcggttttg gtgcgctggt
    32041 tttccagggg ctgctaagca gcgccattga tttagtgttg ctgggggtga tcccggtaat
    32101 tgttctggcg gtgctgatcg atgccctgtt cgatttgctt atcgcactgc tgaaggtgaa
    32161 acgtaatgat tgaatttagc catgtcagca aactgtttgg cgcacaaaaa gccgttaacg
    32221 atcttaatct caattttcag gaagggagtt tttcggtgct gattggcaca tctggctccg
    32281 gcaaatccac caccttgaaa atgattaacc gcctggtgga acatgacagc ggagagatcc
    32341 gctttgccgg agaagaaatt cgctcgctgc cagtactgga gttgcgccgc cggatgggct
    32401 atgccattca atctattggc ctgttccccc actggagcgt ggcgcaaaac atcgctaccg
    32461 tgccgcaatt acaaaaatgg tcgcgggcac ggattgacga tcgtatcgac gaattaatga
    32521 cgctactggg gctggagtca aatttgcgtg agcgttatcc gcatcagctt tccggtggtc
    32581 agcagcaacg tgtgggagtg gcgcgcgcac tggctgccga tccgcaagtc ttactgatgg
    32641 atgaaccttt tggcgcactg gacccggtaa cgcgcggcgc gttgcaacaa gagatgacgc
    32701 gcattcaccg tttgctgggg cgcactattg tgctggtcac tcatgatatt gatgaggcgc
    32761 taaggctggc agaacatctg gtattgatgg atcacggtga agtggtgcag caggggaatc
    32821 cgctgacgat gctgactcgt ccggcgaatg attttgtccg ccagtttttt ggacgtagtg
    32881 aactgggggt gcgcctgctt tcgttacgta gtgtggcgga ttacgtgcgt cgcgaagaac
    32941 gggcagaagg tgaggcactg gcagaagaga tgacgctacg cgatgcgctc tccctgtttg
    33001 tcgcgcgggg atgcgaggtg ctgccggtga tgaacacgca gggccagcct tgcggcacgc
    33061 tgcattttca ggatctgctg gaggaggcgt aagcgtatga aaatgttgcg cgatccgctg
    33121 ttctggctca ttgctttgtt tgtggcactg attttctggc tgccttacag ccagccgctg
    33181 tttgctgcct tgttcccaca actgccacga cccgtttatc agcaagaaag ttttgcagct
    33241 ctggcactgg ctcatttctg gctggtggga atttcgagtt tgtttgcggt gatcattggc
    33301 actggtgccg gaattgctgt cactcgcccg tggggcgcgg aatttcgccc actggtggaa
    33361 actattgccg ccgttggaca gacttttccg cctgtcgcag tgctggcgat tgccgttccg
    33421 gtgatcggct ttggtctgca accagcgatt atcgccttga tcctttacgg tgtgctgccc
    33481 gtcctgcagg cgacacttgc cgggctggga gcgattgatg ccagcgtgac agaagttgcg
    33541 aaaggtatgg gaatgagtcg tggtcagcga ctgcgtaagg tcgagctacc gctggcggct
    33601 ccggtgattc tggcgggcgt gcgaacttcg gtgattatca acattggtac ggcgacgatc
    33661 gcctcaacgg taggggccag cacgctgggt acgcccatca tcatcgggct tagcggattt
    33721 aataccgcgt atgtgatcca gggggcgtta ctggtggcac tggcggcgat catcgcagac
    33781 cgcctgtttg aaaggctggt gcaggcgctt agccagcacg caaaataaag gtataacctg
    33841 cgagcatgac gccaccaatt ccgcctaacg ccataaacag gaacagggcg atgaccccaa
    33901 ttttagctat gcgcatattg cactccttat gttaacgaaa ggattgtaca gtaaagcgca
    33961 tttgttaacg aatcattaaa tgccgagtgg gaaaatatca tggccttgtt cttgccaact
    34021 ggtgagttgc tgctgttggg cggaggttcg attttcaccg caccacacca gcaatgtacg
    34081 gccttcgaat agttcagggc gtagttgatt gagcgagtgg gcgaggacat caatgcgcca
    34141 tccttgttga ctggcaatcc agccctccag ccacagacgg gtggtatcct gaatattcca
    34201 gccaaccacc agcgcatctt taccctgttt tttacgtgcc gaagccagac aaatggcgat
    34261 gtagttgatc agtacgccgt cgaggatcgc cagcagcgcc tggagagtcg gttgttggca
    34321 ctgaagccgt cggcgcagag gaataaacag atgtgtggtg agtgtctggg cggggtaatc
    34381 ctgaccgcgc tctttgatcc acgttcgcag gctatgcaga ttgccgcttt gcaggtaggt
    34441 cagtaatgtt tcttgctgat cgcgccagcc gttctgcaca tcaacatttt cattactgag
    34501 cagcatttta actttgctga cctgcacgcc gttgtcgatc cagcgtttga tctcgcggat
    34561 acggtcaata tcggcatcgt tgaacagtcg atgaccgccg tctgtccgtt gcggtttcag
    34621 caacccgtaa cgcctctgcc acgcgcgtaa cgtgacaggg ttaatatcac aaagcaacgc
    34681 cacttcacca attgtgtaaa gcgccatcgt ctcacccttg ctcgcgaggt cccggtttaa
    34741 ctttagacgc agttttgcga accaggtagt tttgcccgtt ttttgtgcat ctatagggtg
    34801 attttatttt tgccaggcga ttttgagtga tcgtactcac gaattctcat ttttctgcaa
    34861 gagttcaaag aaagttaaac gcaggcaatg tatgttacgc gttttaaagg gaagtgtggt
    34921 ttgcgggtat gtacgatttt aatctggtgt tgctgctgct tcagcagatg tgcgtttttt
    34981 tagtcattgc gtggttaatg agtaaaacgc cattattcat accgttaatg caggtcacgg
    35041 ttcgtctgcc gcataaattt ctctgctaca tcgtcttttc catcttctgc atcatgggca
    35101 cctggtttgg gttgcacatt gacgattcta ttgccaatac ccgtgcgata ggcgcggtaa
    35161 tgggcggctt actcggcggt ccggtcgtcg gtgggctggt tggtctgacc ggcggcttac
    35221 atcgatattc gatggggggc atgaccgcgc taagttgcat gatctcaacc atcgttgaag
    35281 gattgctcgg cggcctggta cacagcatcc tgatccgtcg cgggcgcact gataaagtct
    35341 ttaaccccat taccgccggt gccgtcacgt tcgtcgctga aatggtgcaa atgctgatca
    35401 tccttgcgat cgcccgacct tatgaagatg cggtgcgtct ggtgagtaat attgctgcac
    35461 caatgatggt caccaatacc gtcggcgcgg cgctgtttat gcgtatattg ctcgataaac
    35521 gcgcgatgtt tgaaaaatac acttctgctt tttctgccac tgcgctgaaa gtggcagcct
    35581 cgacggaagg cattttgcga caggggttta acgaagtgaa cagcatgaaa gtggctcagg
    35641 tgctgtatca ggagctggat attggtgcag tcgcgattac cgatcgagag aaattgctgg
    35701 cctttaccgg aattggtgac gaccaccatt tacccggcaa accgatttct tcgacttaca
    35761 ccttaaaagc gattgaaacc ggtgaagtgg tctacgctga tggcaacgaa gtaccttatc
    35821 gttgctcttt gcatccgcaa tgcaaactgg ggtcgacgct ggtaattccg ttgcgtggcg
    35881 aaaatcagcg cgtgatgggc accatcaaat tgtatgaagc caaaaaccgt ttattcagtt
    35941 caataaaccg cacgctgggc gaggggattg cgcaactgct ttcggcgcag attcttgccg
    36001 ggcaatatga gcggcaaaaa gcgatgctca cccagtcaga aatcaaactg cttcacgccc
    36061 aggtgaatcc tcattttttg tttaatgcgc ttaacaccat taaagcggtg atccgccgcg
    36121 acagcgaaca ggccagccag ctggtgcagt atctttccac ttttttccgc aaaaacttaa
    36181 agcggccttc ggagtttgtt actctcgccg acgaaattga acatgtgaac gcttatctgc
    36241 aaattgaaaa ggcgcgcttc cagtcgcggt tgcaggtcaa cattgctatt ccgcaagaat
    36301 tatcccagca gcaattgccc gcgtttaccc tgcaaccgat agtggaaaac gccattaaac
    36361 atgggacatc acaactgctg gatacagggc gagtggcaat cagcgcccga cgtgaggggc
    36421 aacatttgat gctggagatc gaagacaatg ccggtttgta tcaaccggta accaatgcca
    36481 gtgggctggg gatgaatctg gtggataagc gtttacgtga acggtttggc gatgactatg
    36541 ggataagcgt cgcctgtgag cctgatagtt acacccgaat aacgttacga ctaccatgga
    36601 gggacgaggc atgattaaag tcttaattgt cgatgatgaa ccgttagcac gggagaacct
    36661 gcgcgtattt ttgcaggagc agagcgatat tgaaatcgtt ggagagtgtt caaacgccgt
    36721 ggaagggatc ggcgcggtgc ataaactgcg cccggatgtg ctgtttctcg atatccagat
    36781 gccgcgcatc agtggtctgg aaatggtggg gatgctcgat ccggaacatc gtccgtatat
    36841 tgtttttctc accgcgtttg acgaatacgc tattaaagcc tttgaagaac atgcttttga
    36901 ttatctgctg aagccaattg atgaagcgcg actggagaaa acgctggcgc gattgcgtca
    36961 ggagcgcagc aagcaggatg tttcgctgtt accggaaaat caacaggcgc tgaaatttat
    37021 cccttgtacg gggcatagtc ggatttattt gctgcaaatg aaagatgtgg catttgtcag
    37081 cagtcggatg agcggtgtct acgttaccag ccacgaaggg aaagagggct ttaccgaatt
    37141 gacattacgt accctggaaa gtcgtacacc actactgcgc tgccatcgtc agtatctggt
    37201 taatctcgcg catttacagg agattcgtct ggaagataac ggccaggccg agttgatttt
    37261 gcgtaatggc ttaaccgtgc cggtcagccg ccgttatctg aaaagcttaa aagaggcgat
    37321 tggcctgtaa aagactgcta aaatggcttt ttgcctcatc aacacctgaa ggcctcatgc
    37381 taagtaacga tattctgcgc agcgtgcgct acattttgaa agccaataat aatgacctgg
    37441 tgcgtattct ggcgctgggt aatgtcgaag ccaccgcgga acagatcgcc gtctggctac
    37501 gtaaagaaga cgaagagggt tttcagcgtt gtccggacat tgttttgtcg tcattcctca
    37561 acggcctgat ttatgaaaaa cgcggcaagg atgagtctgc tccggcactg gagccggaac
    37621 gtcgcattaa taacaacatc gtgctgaaaa aattacgcat cgcgttttcg ctgaaaaccg
    37681 atgacattct ggctatcctc accgaacagc agttccgcgt ttcgatgccg gaaattacag
    37741 cgatgatgcg cgcaccggat cataaaaact tccgcgaatg cggcgatcaa tttttacgtt
    37801 attttctgcg tggactggca gcgcgccagc atgtgaagaa aagctaagac gggtatggcg
    37861 gccatgcgaa acatggccgc cgacagatta tttcacttct ttaaaaccag cggctttcat
    37921 caccagttcc atttgcgcca tagtgatacc ttttttggca tcttcagcag aaacgttgat
    37981 tcctgaaata ccctgcaggg ctttaaaatc cactttttcc atatcgatag tcacgttttc
    38041 ctgcgcgtag gtatctgtat aggttaattt ttcttcaaca cccgcgatgt ttttgtattt
    38101 ggcgcttaac ggctcaagtg tcttggcagc gtcttctttg gtggttgcac caatagaagc
    38161 aaattgaatt ttggtttcag aagattgctt aagcaccttg tcacctttgt agacataggt
    38221 aatggcaatt tcagtgccgt tcagattggc gctgaatttc ttcgattctt ctttgtcacc
    38281 gcagccagca agagagaaaa ccagaacaga tgcaacaacg agggaaaaca gcttattgaa
    38341 agccttcatg taaaactcca ttttatttaa tcaagaaact ggtgactctc accaggggct
    38401 atatagaata tgcctaatac cgtgacgtga gcagtccgga actggagtag aactcttagt
    38461 aaaaagcact atttcatcct tgttgctgaa gcatggggaa taattgttcg caaagtaaaa
    38521 caccgttatt cattgcttct acccgtgcct cgctttctgt attacgaaat tgtcccaaca
    38581 catgtgccag ccgataaaaa ccgaccgcgg agaggtcatt cgccagcaac tctgcctgac
    38641 taatagcgct ctgttcctga tagcgccagc cgttatggag cagttgaata agtaacgcct
    38701 ggcagcgcat cagcaactga tgagcggtag acggcacagg caaaacgctg gcagaaggta
    38761 gcggtgccac aggcgcagtt tccgcgtcca gcgcccaggc gcgggttttt gtcatcatca
    38821 cccgtggttc cagtgtcaat tgcccatcaa caaaactgac aaagccagaa accagactca
    38881 cggggtcgtc tgtttgttgc aaaagcgccg ccatgcgttc aacggcaaaa ggagaacagg
    38941 cagatgccgg aagggataac gtcaggagat tatcttcacc ttcgccgctg attacctgcg
    39001 catccagcgt ctggcggctg ctatcccaac cgagcgaaat acactcagcg accggcagaa
    39061 taaataagtt atcgacctga ttaagaggcc gtatgcaggc ggggggacgc tggcgtaaat
    39121 attcccgcaa agccacaatg cccggctggc gtaacggcgc gctcaacatt tgccaggcat
    39181 caggcgacag cggcacaacg ctgcttaagc ggttgcgggt agctaacagc agctcgccat
    39241 cggcactgcg ttttgctgct tgtgaaacaa tttgcccacc cgccagtgcg ccagcctgaa
    39301 aactaaacag ccgacgcgta gctgccggtg agttttcctg ttcacttcgc ggccaactgc
    39361 gcgaaaggtg caaaatactg ccggtgtcgg gatcggtaaa ccagatgcgt aaaccataat
    39421 gctcaatatc ctgccagcaa cgcataccta aagacaccag ccgcagatga tcaagctttg
    39481 cttctccggc aatgccagag ccaacgaccg tgcgccacgg cacaggagga acttcaccaa
    39541 cactgtcgcg ccgggccatc tcttgtgcgc aatttaatcg actgtttaat gccgcaagct
    39601 gacgtaagca ttctccggca tgatagtggc tggcgcgagt gtggaaggca tcaacgcttg
    39661 cccgtagctg tcgtagtgat tcactcaccc agcgccagtt gcaggcctct gccgcctgca
    39721 atgcgcggtt gaatgctgcc tcgtagtgaa taagcggctg gctgatgccg ccaagccata
    39781 atgcctggct taattgctga acatattgac gacacgtttg gccttcttcg ctggcaaacg
    39841 gatcgtcaga tgatgtgacg tgttcgctgc gcatctgcca gattaaatgg ttaaattctg
    39901 cttgctgcgc tttggcctcg acgaaggcct gcacagccag tacgacatgt tcgcaaagtg
    39961 tgccttcaat acaatcacaa cgggcgaaac gaatactgct acgggaataa aaacgcacat
    40021 cgctcatcgg taagcgggca gagggaattt cgcccggcgc acagaacaac tcaatggtga
    40081 tgcctttagc gaccagcgcc tgcgcgcgtt tgcgggtggc atcgggcagg gtagccagtt
    40141 cttccagcca gattgccgga tcccactctt cttctttttc cgtgggctgg acggtggcac
    40201 aaagtcgttg ataacttaac accagcatca cgcgatgacg gcacatgccg ctggccccgc
    40261 agctgcactg agcctctttc agtgcctggc tgttcgccag ctgggtacgg acaccgtcac
    40321 tgaaggtggc gattaaagcg ccgttctcat ggctgatttc cgggacgttg ccattttcca
    40381 gttccttaag actgcgctta acaaaaccgg cattgcttaa cgccgtcagt gcctgcggtg
    40441 tcagttctaa taattccgga cgtagtgaat tcatgactga agattctccg caagccatga
    40501 tgccagctcg cccggcgtca tggcggctat ttgtgcgccg acattaacca gcgcctgggc
    40561 cgtatcgcgg tcatagcaag gcgttgcggt gctatcgagc gctgccagtc ccagcacttt
    40621 gatgccgctc tggacacact ttttcacctg atgcgtcagc aatgatgatg aacccccttc
    40681 gtaaaaatcg ctcacgagga taatgacgct ttttgctggt tgttcaataa gttgccgacc
    40741 atactccacg gcactggcga tattggtccc gccgcccaac tgtactttca ttaataactc
    40801 taccggatcg gcaacgtctg ccgtgagatc aaccacgctt gtgtcaaacg ccaccagatg
    40861 ggtacgaatg ccgggtaact gccacaaaca ggccgccatc accgcagagt ggatcaccga
    40921 gtccaccatc gatccgcttt gatcaaccag taagaccagt tgccattgtt cgctttggcg
    40981 tttaatgcgg ctgttaaagc ggggggattc gatatacaac ttgccgtgtt gcgggtgcca
    41041 gtgttgcagg ttggcgcgca gagtactttt gaaatcaaag tttcgcgcca gtggaattaa
    41101 tgagcggcga cggcgatcgc ggacaccaga aaaagcctga cgaacttcct ttgccagtcg
    41161 cgccataatt tcttcaacaa cctggcgcac tatctggcgg gcggcagcca gtacttcggg
    41221 attcatcaga tgtttggtgt gcaaaacggc gcgtagcagg ctttccgaag gctgcatacg
    41281 ttccagcacg tcgagattcg tcaccacatc ttcaatgccg tagcgcagta cggcatcgct
    41341 ttccagtcgc tcaatcacct gttgcggaaa cagagtgtga atactgttga tccactcagg
    41401 ggtggtgaga tttgagccac ctaatccacc agagcgttca ccacgctgga gccgttcagg
    41461 atcgcgccca tacagccact ccagcgcgtg gtctatctgc cgggcgttgt catccagccc
    41521 acaaagcgtc gtttctgccg cttcgccaag aattaatcgc cagcgttgta gctcacgggt
    41581 ggtcagaaga tcgttcagtt cagacatgga aaactcccca gttttgcagc gatgccagtg
    41641 cctgctgttc gagttgttga tgatgtgcaa tggcctgtgg tggacaatgc aacggcattt
    41701 gcagcgccga aacgggaagt tgcgccagtt gataatgctc aagcacctga tgcgccagcg
    41761 tcccgcgttc tcgtggtggt agccaggcca tcgccgcgcg taaatcgggc agggcattga
    41821 taaaatcggc atcacttagt tgatttaaat gactgctgaa accggcgata aatgtcggct
    41881 gacaggccag ttgatggcgg gccagcgcca gcaaaccgtg cagcgcttcg ccggattgtg
    41941 ccggggataa ctgcgccagc atcgtcagcg ccgcttcggc actggcgtta ggatgttcca
    42001 gacgcatcag tgcgccatga gccgcgccgc gatccagcgc cggggcatga attgcctgac
    42061 tgcgtcgctc cagtaaggct accgccgcag aaagagaaat gccggggagt tgcacaccgc
    42121 tatgtagatc gcgcaggata tgacacaacg cttgccagct atgcaggtga gcgtgaaact
    42181 ccttttcatc tggtctgccg ttagattcac atagccacag cgtgcgatcg atagccgcgc
    42241 ataacgtcgt ttgtaatatc tgcgcgcctt gcataccgct aatttcatcc agccgccata
    42301 aggcatatag cacttccagc gcctggccca tttcggcaaa ttgattttcc tgggcgatta
    42361 ataatgtgag ttgctccagt aattgttgac tgaaggacgc cagacccgct aacgccgcct
    42421 ggctaagaca tgtggtgata ctgccgattc cacccgcgtc cagcatatcg gcttctaatt
    42481 tatggcgtgc ggcttcctgg agtgttgcac caaagcaggc ggcctcaatc aatgccgcat
    42541 gctggctaag cgggcgggtt aatttccagc gttcttcacc gttgcctgca agtgtcagtg
    42601 tgcttccctg ctggcgcaca atccctggga tctccagtat tgccagccga tgtaacacct
    42661 gactttgcgc tagtccattg gggttaaagc gatttaaggt aagctccgcc ggaagggaga
    42721 ttgcggtacg ttccagttcg caggtgacat cttttggcag aggaggttgt ggcgtagaag
    42781 gggcaagttt accgaatccg tcacccgcta atgtgtctat caacgttagc agaatcggat
    42841 cggtgtcggg atgaataacg ccgcgatagc tccacggcaa cggcgcattc agggcttctt
    42901 taatcagcga tcctgctatg gcatccagcc agtcagtgcg taacggtagt gtatgaccgc
    42961 gcaactgtgc cagtgccatc gcatgcagat gagcggcagc catatccgcg gtcgaagcag
    43021 gcaacttgtg ctggcgcaaa cgggtgagaa ccgtttttag cagttgttct cccgcctgct
    43081 gtaagcccca ctgccagcac cagttttgcc agaccggggc aggcattccg gaaaggtatc
    43141 ctgccagcac atcaaggcgc ttttcactgt agggggtgag ataacaacct gtaacggcat
    43201 ctgccagcga cggcaattct ggcgtgttaa tttcctgcgg gcattcgcgc cacatttttg
    43261 ccagtaccgg agcgtgccag ccaccgcaga caactaacac gtcgccatta ttttgctgca
    43321 tcgcccatcc aatccagcgg gccataaagg cttcgcgcag acgattgaga gcatctccgg
    43381 gggagtcgcc ccgcagttgg gcaaaatagt gcgccagtgc agagggtaat gcagtttgct
    43441 ggctttcatc ttcgaacaag tgatcccaca gggtgtcgct gttatccatg cgggtggcac
    43501 gcagcagcaa ggcctggctt tcctcttgcg tatcaggcga atcgtccact tcttcactct
    43561 gcgcccagca aggcaaatcg atgaagtaag tttgtgcctg aatacgacgt gcggcttgta
    43621 gcgcctgcca ctccggcgaa aattcagcaa atggcgtcca ggcaccacgc tcgggggctg
    43681 caccgtcctg atactggcaa taactgtaaa tagctaccgg aagctggtgg gctaaaaaca
    43741 gttcgtctac ccggtcatta aaatcagccg ggccttcaat caatacgtat cgtggccgct
    43801 ggctttcgat taacgatttc accagccggg cgcaggccgg actatgatgc cggatgccga
    43861 caattaacgg ctcgctcatg catctttcct cacggcaggc ggtggcgggc ttgataataa
    43921 gcctgccagt gagcttcttt atgtgtggcg actcgttgtt cgaagtaacg gcgcagacga
    43981 gcgcgatctt cttcgttatc tttgacgatg gttccggcaa tgcagtccac caggtcggct
    44041 ggctcacctg cgcgattcgc taaaaaccag gcgcggacgc ctacggcgtg ggccacgttc
    44101 acggcttcag cggtggacat aatcgctgtt agtgtatcca ttgacgtttt cttttcgccg
    44161 ttggcacgca gatcgcggaa ggtgcggacc agtaactcca gtaccgcgtc aggcacttta
    44221 tgcggaatac cgctatgggc cagcaaacgc gccgaagcac tggcgacaag ctccagctcc
    44281 tgggcgaaat ccataatcgg aaagacagtt tcaaaatcga agcgacgctt tagcgcagca
    44341 ctcatttcat tgacgccgcg atctcgggta ttggcagtgg caataatatt aaagccttct
    44401 cgcgcataga gctgactggc ttcaccagtg agttccggca ccgtcatcac cctgtcagaa
    44461 agcataccta acagacagtc ttgcacttcc agcggtgtac gcgttatctc ttcaaaacgg
    44521 acgattttgc cgtcgcgcat tccctggtaa agcggggcag ggaccagcgc ttccgttgat
    44581 gggccatgat tgataagcag agcgtagttc cagccgtact tgatttgatc ttcagtggta
    44641 gatgcgccgc cctggatggt taacccggca tcgccgctaa tagcggttgc cagtaattca
    44701 gacaggagag attttgcggt gccaggttcc cccaccaaca tcaaaccgcg tccggtggca
    44761 agagtgacta acatacgttc gacagcggag ggactaatca ccactttacg gctaatattt
    44821 tgcgcgctat cgccaaggat aaaagcacgg gccgcaggca aacttaactg ccaaccgggc
    44881 gggcaaggag cgttatcatt ttgttttaat tttgccagtt catcggcgta taacacagca
    44941 gcaggcggac gctgcagatg attattctgc ggtgacatat attcatctct gaaatgtatt
    45001 aatgaaataa acgatatttt acgcgcaata aatgtttggc gcattagttg tttgagcaaa
    45061 aaatatctgg aaagcagatc gcagtaaata aaaaaacctg ctggtgaggc aggttctttt
    45121 atttactatt ttcaggccta acaccacagc ctgtgcaaca tcgtgttcca acgaacgaat
    45181 tataaccacg gctggacccc gttgcaatta agataatgag atttataatc cagaccgttg
    45241 ggacgttgcg ccggatcgaa cctatttcaa gtcctggcta ccggacgggg ggtgctgcag
    45301 gtggtgccct gatccggagt gcaacatcgt ggtccaacat acgtaaacca gtatcgacag
    45361 gcagtgtaag ggtaaggtgc tggtgttgcg ctccttaata aggagagcgg atggactcgc
    45421 tgacacaaaa gttaaccgtg ctcattgctg tactggagct
//
LOCUS       DBANUU010000023        44758 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000023 DBANUU010000000
VERSION     DBANUU010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.023"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACIE68_001231"
     CDS             complement(<1..182)
                     /locus_tag="ACIE68_001231"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5907579.1"
                     /translation="MSKGALYEFNNPDQLKIPLPHKHIASTFNDIMSKDVGYAYVSLL
                     YACPLKTHSLRLNPFS"
     gene            502..1590
                     /gene="pdeL"
                     /locus_tag="ACIE68_001232"
     CDS             502..1590
                     /gene="pdeL"
                     /locus_tag="ACIE68_001232"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HFX5907580.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGSNSHRYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGGETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFVSEWVMKAGG"
     gene            complement(1632..2564)
                     /gene="yahB"
                     /locus_tag="ACIE68_001233"
     CDS             complement(1632..2564)
                     /gene="yahB"
                     /locus_tag="ACIE68_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFX5907581.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(2656..3153)
                     /gene="yahC"
                     /locus_tag="ACIE68_001234"
     CDS             complement(2656..3153)
                     /gene="yahC"
                     /locus_tag="ACIE68_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFX5907582.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGISGFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            3411..4016
                     /gene="yahD"
                     /locus_tag="ACIE68_001235"
     CDS             3411..4016
                     /gene="yahD"
                     /locus_tag="ACIE68_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFX5907583.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDLTCLNPFLMSCLNGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            4056..4919
                     /gene="yahE"
                     /locus_tag="ACIE68_001236"
     CDS             4056..4919
                     /gene="yahE"
                     /locus_tag="ACIE68_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFX5907584.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFNGLFRHGDKVQFDEQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALVLFIPGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHSFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            4909..6456
                     /gene="fdrA"
                     /locus_tag="ACIE68_001237"
     CDS             4909..6456
                     /gene="fdrA"
                     /locus_tag="ACIE68_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFX5907585.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            6456..7874
                     /gene="yahG"
                     /locus_tag="ACIE68_001238"
     CDS             6456..7874
                     /gene="yahG"
                     /locus_tag="ACIE68_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFX5907586.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEATELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            8017..8967
                     /gene="yahI"
                     /locus_tag="ACIE68_001239"
     CDS             8017..8967
                     /gene="yahI"
                     /locus_tag="ACIE68_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFX5907587.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            8977..10359
                     /gene="yahJ"
                     /locus_tag="ACIE68_001240"
     CDS             8977..10359
                     /gene="yahJ"
                     /locus_tag="ACIE68_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFX5907588.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            10575..10649
                     /locus_tag="ACIE68_001241"
                     /pseudo
     CDS             10575..10649
                     /locus_tag="ACIE68_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10736..11785
                     /gene="yahK"
                     /locus_tag="ACIE68_001242"
     CDS             10736..11785
                     /gene="yahK"
                     /locus_tag="ACIE68_001242"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFX5907589.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            12028..12843
                     /gene="yahL"
                     /locus_tag="ACIE68_001243"
     CDS             12028..12843
                     /gene="yahL"
                     /locus_tag="ACIE68_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFX5907590.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            13255..13500
                     /gene="yahM"
                     /locus_tag="ACIE68_001244"
     CDS             13255..13500
                     /gene="yahM"
                     /locus_tag="ACIE68_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFX5907591.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(13517..14188)
                     /gene="yahN"
                     /locus_tag="ACIE68_001245"
     CDS             complement(13517..14188)
                     /gene="yahN"
                     /locus_tag="ACIE68_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFX5907592.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            14335..14610
                     /gene="yahO"
                     /locus_tag="ACIE68_001246"
     CDS             14335..14610
                     /gene="yahO"
                     /locus_tag="ACIE68_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFX5907593.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(14708..16294)
                     /gene="prpR"
                     /locus_tag="ACIE68_001247"
     CDS             complement(14708..16294)
                     /gene="prpR"
                     /locus_tag="ACIE68_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFX5907594.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCKLEEDMQQGRFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            16532..17422
                     /gene="prpB"
                     /locus_tag="ACIE68_001248"
     CDS             16532..17422
                     /gene="prpB"
                     /locus_tag="ACIE68_001248"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFX5907595.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEAKLDDLFARSQVK"
     gene            17467..18636
                     /gene="prpC"
                     /locus_tag="ACIE68_001249"
     CDS             17467..18636
                     /gene="prpC"
                     /locus_tag="ACIE68_001249"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFX5907596.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            18670..20121
                     /gene="prpD"
                     /locus_tag="ACIE68_001250"
     CDS             18670..20121
                     /gene="prpD"
                     /locus_tag="ACIE68_001250"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFX5907597.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            20161..22047
                     /gene="prpE"
                     /locus_tag="ACIE68_001251"
     CDS             20161..22047
                     /gene="prpE"
                     /locus_tag="ACIE68_001251"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFX5907598.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            22379..23638
                     /gene="codB"
                     /locus_tag="ACIE68_001252"
     CDS             22379..23638
                     /gene="codB"
                     /locus_tag="ACIE68_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFX5907599.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            23628..24911
                     /gene="codA"
                     /locus_tag="ACIE68_001253"
     CDS             23628..24911
                     /gene="codA"
                     /locus_tag="ACIE68_001253"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFX5907600.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(24951..25850)
                     /gene="cynR"
                     /locus_tag="ACIE68_001254"
     CDS             complement(24951..25850)
                     /gene="cynR"
                     /locus_tag="ACIE68_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFX5907601.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            25960..26619
                     /gene="cynT"
                     /locus_tag="ACIE68_001255"
     CDS             25960..26619
                     /gene="cynT"
                     /locus_tag="ACIE68_001255"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFX5907602.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            26650..27120
                     /gene="cynS"
                     /locus_tag="ACIE68_001256"
     CDS             26650..27120
                     /gene="cynS"
                     /locus_tag="ACIE68_001256"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFX5907603.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            27126..28307
                     /gene="cynX"
                     /locus_tag="ACIE68_001257"
     CDS             27126..28307
                     /gene="cynX"
                     /locus_tag="ACIE68_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFX5907604.1"
                     /translation="MRNHQKTFTILLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(28410..29021)
                     /gene="lacA"
                     /locus_tag="ACIE68_001258"
     CDS             complement(28410..29021)
                     /gene="lacA"
                     /locus_tag="ACIE68_001258"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFX5907605.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLLKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKQYYFKDYKVESSV"
     gene            complement(29087..30340)
                     /gene="lacY"
                     /locus_tag="ACIE68_001259"
     CDS             complement(29087..30340)
                     /gene="lacY"
                     /locus_tag="ACIE68_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFX5907606.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTVNNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(30392..33466)
                     /gene="lacZ"
                     /locus_tag="ACIE68_001260"
     CDS             complement(30392..33466)
                     /gene="lacZ"
                     /locus_tag="ACIE68_001260"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFX5907607.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHIIPQ
                     LTTSETDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(33589..34671)
                     /gene="lacI"
                     /locus_tag="ACIE68_001261"
     CDS             complement(33589..34671)
                     /gene="lacI"
                     /locus_tag="ACIE68_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFX5907608.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(34748..35581)
                     /gene="mhpR"
                     /locus_tag="ACIE68_001262"
     CDS             complement(34748..35581)
                     /gene="mhpR"
                     /locus_tag="ACIE68_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFX5907609.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLAARPGDDYQLAREPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            35772..37436
                     /gene="mhpA"
                     /locus_tag="ACIE68_001263"
     CDS             35772..37436
                     /gene="mhpA"
                     /locus_tag="ACIE68_001263"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFX5907610.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGENEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTTGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            37438..38382
                     /gene="mhpB"
                     /locus_tag="ACIE68_001264"
     CDS             37438..38382
                     /gene="mhpB"
                     /locus_tag="ACIE68_001264"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFX5907611.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            38400..39266
                     /gene="mhpC"
                     /locus_tag="ACIE68_001265"
     CDS             38400..39266
                     /gene="mhpC"
                     /locus_tag="ACIE68_001265"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFX5907612.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLKWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            39276..40085
                     /gene="mhpD"
                     /locus_tag="ACIE68_001266"
     CDS             39276..40085
                     /gene="mhpD"
                     /locus_tag="ACIE68_001266"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFX5907613.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            40082..41032
                     /gene="mhpF"
                     /locus_tag="ACIE68_001267"
     CDS             40082..41032
                     /gene="mhpF"
                     /locus_tag="ACIE68_001267"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFX5907614.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPKSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            41029..42042
                     /gene="mhpE"
                     /locus_tag="ACIE68_001268"
     CDS             41029..42042
                     /gene="mhpE"
                     /locus_tag="ACIE68_001268"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFX5907615.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            42218..43429
                     /gene="mhpT"
                     /locus_tag="ACIE68_001269"
     CDS             42218..43429
                     /gene="mhpT"
                     /locus_tag="ACIE68_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFX5907616.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            43531..43710
                     /locus_tag="ACIE68_001270"
                     /pseudo
     CDS             43531..43710
                     /locus_tag="ACIE68_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 family protein"
     gene            complement(43716..>44758)
                     /locus_tag="ACIE68_001271"
     CDS             complement(43716..>44758)
                     /locus_tag="ACIE68_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HFX5907617.1"
                     /translation="NIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSD
                     IREQKRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLI
                     VSDAGFKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGY
                     KRLTKSNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWILATNLPVE
                     IRTPKQLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTC
                     WLAGVHAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDSLVAATLLTQ
                     NLFTHGYVLGKL"
BASE COUNT        10598 a        11407 c        12229 g        10524 t
ORIGIN      
        1 ttgctgaatg gattcagtct taatgagtgg gtttttaagg gacaggcata gagtaatgat
       61 acgtatgcat aaccaacatc tttactcatt atgtcattga atgttgacgc tatgtgttta
      121 tgagggagag gtattttcag ttgatctgga ttgttaaatt catataatgc gcctttgctc
      181 atgaatggat gccagtatgt agtgggaaat tataaatatt gaaatagtcc aactacttct
      241 ttattaccaa aaatgagtat ctgaatttta atattgcatt cttgcgtgat tatctcctga
      301 gtttgacttg tgattacctt tttaaggtat ttagcgtaac tgtttttgag cgagcatcag
      361 aggtaaagat aatcttcttg atagtgatgt gggatgttat acgtatggca tcgctgatgt
      421 ttatggttac cccttatgtg tgctcaggaa tcgacaggta atcactcata ctgaacagcg
      481 ataaaagata aaggtgtgtt catgaattca tgtgattttc gtgtttttct gcaagagttc
      541 ggtacaacgg ttcatttgtc attgcctggt agcgtatccg agaaagaacg actgctactc
      601 aagctgctga tgcagggaat gtctgtaaca gaaatatcac agtacagaaa tcgcagtgca
      661 aagacaattt cacatcaaaa gaaacagctc tttgagaaac tggggattca gagcgatatt
      721 actttctggc gcgatatttt ctttcagtac aatccggaga tcatatccgc cacggggagt
      781 aatagtcaca gatatattaa tgataatcac tatcaccata tcgtcacgcc tgaagccatc
      841 agtctggcgt tggaaaacca cgaattcaaa ccgtggatcc aaccggtttt ctgcgcgcag
      901 actggggtac tgacgggctg tgaggtgctt gtccgctggg aacatccaca aacgggaatt
      961 atcccaccgg atcagtttat tcctctggcg gagtcatctg gtcttatcgt cataatgacc
     1021 cgccaactga tgaaacagac tgcggatatt ctgatgccgg taaaacattt gctgccggat
     1081 aatttccata ttggtatcaa cgtctcggcg ggttgttttt tggccgcggg atttgaaaaa
     1141 gaatgcctga acctggtcaa gaaattagga aacgataaaa tcaaactggt tctcgagcta
     1201 acggaacgta accctattcc ggtaacgcca gaagccagag cgatatttga cagccttcat
     1261 cagcacaaca ttacctttgc gctggatgac tttggtacgg gttatgcgac ctatcgttac
     1321 ttgcaggcgt tcccggtcga ttttattaag atcgataagt catttgtgca aatggcgagt
     1381 gtcgacgaaa tctccggtca tattgtggac aatattgtcg aactggcgcg taagcctggt
     1441 ctgagtatcg tagcggaagg gggagaaacc caggagcagg cggatttaat gatcggtaaa
     1501 ggcgttcact ttttgcaggg ctatttgtac tccccgccag taccgggtaa taaatttgtt
     1561 tctgaatggg taatgaaagc tggtggttga tgtaaaccgc tattcacagc gcatcgggag
     1621 gttggcagcg attaattctc ccgatgcagt aaatcctgat agatccccgt taatatgcca
     1681 tttggcgcaa attgcttttt aatccactgc gtcacctgac cggtggcaga gtgctgggtt
     1741 gcgagtaaca tgcgtgaatc ctggcgcggg ttatggattt gtcgggtaac cagcaaggat
     1801 tgcgccatcg cctcacggac catgtaatcc ggcaaaaaac caattccttc acctaaaatc
     1861 tggcactgac atttggtgtt gaaatctggc accagaattg actcctgccc atgcagcaac
     1921 cagcccactt ttttattaat cgtatgtgcg gtgtcctcca ccataatatt agggtagaga
     1981 cgcagttggc tttcggcgat gggttccggc acaaaggcta acgggtgatc cggggcgata
     2041 gcaaaagccc agcgaatcgc gccaatttca gtgtaatcaa taccgccgcc gtccagtagt
     2101 gtatcgggtg cgccgatggc gatattggcc tgattattaa taatcgcatc ccagacgccg
     2161 ttatacactt cggtcgtcac ggtaatctgg caggtaggaa actgcttttt cagcacctgt
     2221 aataagcgcg cggtgtgttt gggcgtataa agcagctgat taatacaaat gcgcacacgc
     2281 gcttctatgc cctgcgaaat agtatctatg ctacgtttga tggcataaaa atcattcagc
     2341 aggtcggtag ctttgcggaa aaaataacgc ccggactccg ttaactcaat gctgcgggta
     2401 ctgcgagtga acagcaccac atccagcccc gtctccatac gcttaatggt gtagctaatg
     2461 gcggaagtgg ttaaacccaa ctcttctgcg gctttactga agctgccaaa acgcgcagcg
     2521 gtagtaaatg ccaacaggtt ttcttcggta aaaattgagt tcattaaacc attctcgcca
     2581 ttatctacta tgaatttctc cagctatgac tgtagaggta tcggttaaag atagataatc
     2641 atttttgaat aacttttaat acccgtcgcg tttagtcgtc gcttcgctta atttttgtcc
     2701 accatattct gaaattatcc ccacgcagtt acccgccaga aaacccagta acgtcaccgt
     2761 ccagttcatc ccgctggcaa aaaacagcgt catgccgaga aagccaccgg gaataaatga
     2821 cagtaaccag aaacgccctt gccagaccac taccgctgag aaaggcacgg tagtaatcac
     2881 gctcgcccag aacccagaaa tcccactcac cgaggccagc caaccggcag ccagcgcggc
     2941 gaaaaatgcc cacaccatcc cggaataatt aaccgccaga cttctgataa accccgattt
     3001 accgccgcct gcggcataaa aactgcaaaa ggcgacgaag ccaatggttc ctaataattc
     3061 ccatccctga gagagcccga catgtgaaga cactaactgc caaagccctg cacaaatacc
     3121 gacggtgaca cccgttgccg tgagtccgtt catcttgttc ccctctggtt agacatggac
     3181 gaaagctact cttacatgtg cagactatag agaaataaaa cctgattgag tgaaagggta
     3241 tcgggtcaaa gaaacaaata ttgcactacc gcacactgcg aaaagattgt tgaacatctt
     3301 tcaacaaaaa ggcgaaatac ggcgaattaa atttaagcgg gttcttttat cagattatcg
     3361 ttgctattct caattcatcg aaaacagcca caaaaaccag gggaatgtga atgacgataa
     3421 aaaatctacc tgccgactat ttattggctg cacaacaggg cgatatcgat aaagtaaaaa
     3481 cctgtcttgc gctgggtgtc gatattaata cctgcgatcg ccaggggaaa acggcaatta
     3541 cgctggcaag tttatatcag caatatgctt gcgttcaggc attaattgat gccggagcgg
     3601 atattaataa acaagacctg acatgtttaa atcctttttt aatgagctgt ctgaacggtg
     3661 atttaacgct gctacgaatt attttaccgg ctaaaccaga ccttaattgc gtcacccgtt
     3721 ttggcggtgt cggcctgacg cctgcctgtg aaaaaggcca tttaagtatt gtaaaagagc
     3781 ttttggcgca tacggaaatt aacgttaacc agaccaacca tgttggctgg acaccgctgc
     3841 tggaagcgat tgtgcttaat gatggcggga ttaaacagca ggcgattgtc cagttattgc
     3901 tggaacacgg tgccagcccg catctgaccg ataaatatgg caaaacgcca ctggaactgg
     3961 cgcgggaacg gggctttgaa gagattgcgc agttactgat tgccgcaggt gcataaaccg
     4021 ggaggcttgc tatcaacaca ccagaaagac ggtgtgtgtg ggcgctaact gcggatgcgg
     4081 attttctggc gcagcggggg caaggacagg ttgaacaggt ctttgccaga gcggtaaata
     4141 tcgcactccc ggctcgccag cagttgctga cgctgctttg tgaagagtac gacaatgcgc
     4201 caaacagttg tcggttggca ctcactcact ttaatggtct gttccggcat ggtgataagg
     4261 ttcagtttga cgagcaaggc attacggttg gtcaacatct tcatatagag atgagtcgtt
     4321 gtcggcgttg gctgtcccca accttgcaaa tgaccgctgt gaattttcac cttatcgcct
     4381 ggcaacagtg gcacgacatt attcatcagc acctggggga aaatgaaacc ctgtttaatt
     4441 atcgcggcga taatccgttt tatcaggcgt taaataaaga attacatatt aaacgacggg
     4501 cagttattca ggccgtaaac gataaacaaa atatcgcctc agcggtcgcc agtatgatgg
     4561 ggttagggat tggccttacg ccatcagccg acgattattt aacaggtctg gcgcttgttt
     4621 tatttattcc cgggcatccg gcggaaaaat acaaagagga attttatctc ggtctgcaac
     4681 gcggcagaaa taataccaca ttattaagtg ctataacgct ggaagccgca ttacaacaac
     4741 gttgccggga aaatattcat agttttattc acaacattat ttatgacatc cctgggaacg
     4801 caactcaggc aatagaaaaa attaaacata ttggctccag ttccggctgc gacatgctgt
     4861 atggcatggc cgatggttgt gcgctgagcc aaacctacgg agggaattat gtcagttaaa
     4921 atagtcatta aaccgaatac ctattttgat tctgtctcgc tgatgtctat ctccacgcgt
     4981 gcaaataaac tcgacggcgt cgagcaggca tttgtggcga tggcgaccga aatgaacaaa
     5041 ggtgtgctga aaaatttagg actgctgacg ccggagctgg aacaggcgaa aaacggcgac
     5101 ctgatgattg tcatcaatgg taaatcgggt gcggacaacg agcagttgct ggtggagatt
     5161 gaagaactgt tcaacaccaa agcgcaaagc ggctcgcacg aggcgcgtta cgccactatt
     5221 gccagtgcca aaaagcatat cccggaaagt aacctggcgg tgatttcggt caacggtctg
     5281 tttgccgctc gcgaagcgcg tcaggcgctg caaaacgatc tcaacgtgat gctgttttcc
     5341 gataacgtct cggttgaaga tgaactggcg ctcaaacaat tggcccatga aaaagggctg
     5401 ctgatgatgg ggccggactg cggcacggcg attatcaacg gcgcggcgct ctgctttggt
     5461 aacgccgtgc gtcgcggtaa catcggtatt gttggcgcat ccggcaccgg cagccaggag
     5521 ctgagcgtcc gcattcatga atttggtggc ggcgtttcac aactgattgg caccggcggg
     5581 cgcgacctga gcgagaaaat cggcggcctg atgatgctcg acgccatcgg gatgctggaa
     5641 aacgatccgc aaactgaaat cattgtgctt atctccaaac cgcccgcgcc tgcggtggcc
     5701 cgtaaagtgc tggaacgcgc gcgcgcctgc cgcaagccgg tggtggtctg cttcctcggt
     5761 cgcgttgaaa cgccggtgga tgagcagggg ctacagtttg cccgcggcag caaagaggcg
     5821 gcgctaaaag cggtgatgct ctccggcgtg aaacaggaaa atctcgacct gcatacgctt
     5881 aaccagccgt tgattgctga tgtgcgtgcg cgtctgcaac cgcagcagaa atacatacgt
     5941 ggcctgttct gcggcggcac gctgtgcgac gaaaccatgt tcgcggtgat ggaaaaacat
     6001 ggcgatgtct acagcaacat tcagcccgat cctgaattcc gcctgcaaga tatcaaccgc
     6061 agcatcaaac acaccttcct cgactttggc gatgacgact tcaccaacgg caagccgcat
     6121 ccaatgattg accccaccaa ccgcatcagt cgcttgatcg aagaagcgcg cgatccagaa
     6181 gtggcggtga tcgtgatgga ttttgtgctc ggatttggat cgcatgaaga tccggtcggc
     6241 tccaccatcg aggcgatcaa agaagcgaaa gcgatcgccg ccgccgaagg acgcgagttg
     6301 atcattctcg cctatgtgct gggcaccgat ctcgatacgc catcattaga acaacaaagc
     6361 cagatgctgc ttgatgccgg agtgattctg gcgagcagca gcaccaatac cggattgctg
     6421 gcgcgtgaat ttatctgcaa aggggaggaa gcctgatgag ccagtcactg tttagccaac
     6481 cgttgaacgt tattaacgtc ggtatcgcca tgtttagcga tgacctgaaa aagcagcatg
     6541 tagaagtgac tcaactcgac tggacgccgc cggggcaggg caatatgcag gtggtgcagg
     6601 cgctggataa cattgccgat tcgccgctgg cagacaaaat cgccgccgct aaccagcagg
     6661 cgctggagcg cattatccag tcgcatccgg tgctgattgg ttttgatcag gcgattaacg
     6721 tggtgccggg catgacgccg aaaaccattc ttcacgccgg gccgccgatc acctgggaaa
     6781 aaatgtgcgg cgcgatgaaa ggcgcggtca ccggggcgtt ggtgttcgaa gggctggcga
     6841 aagatctcga tgaggcgact gaactggcgg cctccgggga gatcaccttc tcgccgtgcc
     6901 acgaacacga ctgcgtggga tcgatggcgg gcgttacctc ggcctcgatg tttatgcaca
     6961 tcgtgaaaaa caaaacctac ggcaacatcg cttataccaa tatgagcgag cagatggcga
     7021 agattttgcg tatgggtgcc aacgatcaga gcgtgatcga ccgcctcaac tggatgcgcg
     7081 atgtgcaggg gccaatgctg cgcgacgcga tgaaaattat cggtgagatc gatctgcgct
     7141 taatgctggc gcaggcgctg catatgggcg atgagtgcca taaccgcaat aacgccggga
     7201 cgacgctgct gattcaggca ctgacgccgg ggatcattca ggcaggctat tccgtcgagc
     7261 aacagcgcga agtgttcgag tttgtcgcca gcagcgacta cttctccggc ccgacgtgga
     7321 tggcgatgtg taaagcggcg atggatgcgg cgcacggcat cgaatacagc accgtggtca
     7381 ccaccatggc gcgtaacggc gtcgagttcg gcctgcgggt cagcggcctg ccggggcagt
     7441 ggtttaccgg cccagcgcag caggtcatcg gcccgatgtt tgctggttat aagccggaag
     7501 attcggggct ggatatcggc gacagcgcca tcacagaaac ctacggtatt ggcgggtttg
     7561 ccatggcgac cgcgcctgca atcgtcgcac tggtgggcgg cacggtggaa gaggctatcg
     7621 atttctcccg tcagatgcgc gaaatcaccc ttggcgaaaa ccccaacgtc accattccgc
     7681 tgctcggttt tatgggcgtg ccgtcggcaa tcgacatcac ccgcgtgggt agcagcggca
     7741 ttctgccggt gatcaacacc gccatcgccc ataaagatgc gggcgtcggc atgattggcg
     7801 cgggcattgt gcatccacca ttcgcctgct tcgagaaagc cattctcggc tggtgcgaac
     7861 gttacggcgt ctgacttaca tgtttatgcc ggatgcggtg tgaacgcctt atccggccta
     7921 cggatggccc gagaattgta ggcctgataa gcgtagcgca tcaggcagtc tggcgttggt
     7981 cataacctaa tcaccctctg tagcggacat aacaacatga aagagcttgt ggtcgttgcc
     8041 attggtggca acagcattat caaagataac gccagccagt cgattgagca tcaggcggag
     8101 gcggtgaaag ccgtggccga tacggtgctg gaaatgctgg cttccgatta cgacattgtg
     8161 ctgacccacg gtaacgggcc gcaggtcggg ctggatttac gccgtgcgga gattgcccac
     8221 gagcgcgaag ggctgccctt aacgccgctg gcgaactgtg tggcagatac gcagggcggc
     8281 atcggctatc tgatccaaca ggcgctgaat aaccggctgg cgcgtcacgg cgagaaaaaa
     8341 gccgtcaccg tggtaactca ggtggaagtg gataaaaacg atccaggttt tgcccatccc
     8401 accaagccca ttggtgcatt cttcagtgaa agccagcgtg acaaactaca aaaggcaaac
     8461 cctgactggt gttttgttga agatgccgga cggggctatc gccgcgtggt cgcctcgccg
     8521 gaaccgaaac gtattgtcga agcacccgcc attaaggcgc tgatccaaca aggttttgtc
     8581 gtaattggcg cgggcggcgg tggaattccg gtagtgcgta ctgaagcggg ggattaccaa
     8641 agcgtggacg cggttattga caaagatctc tctaccgcgc tgctggcccg tgaaattcac
     8701 gccgacattc tggtgatcac caccggcgtc gaaaaagtct gtatccactt tggtaaaccg
     8761 cagcagcagg cgctggatcg ggtggatatt gccaccatga ctcgctatat gcaggaaggg
     8821 cattttccgc ccggcagcat gttgccaaaa atcatcgcca gcctgacgtt cctggagcag
     8881 ggcggcaaag aagtgattat caccacgccg gaatgcctgc ctgcggcgct gcgcggtgaa
     8941 acgggcaccc atattattaa aacgtaagga cgtaggatga aagaaagcaa tagccgccgt
     9001 gaatttctga gccagagcgg taagatggtc accgccgccg cgctgtttgg tacctctgtg
     9061 ccgctcgccc atgcggcggt agctggcacc ctaaactgcg aagcgaacaa caccatgaaa
     9121 atcactgacc cgcattacta tctcgataac gtgctgctgg aaaccggttt tgactacgaa
     9181 aacggtgtgg cggtgcagac ccgcacggcg cgccagaccg tggagattca ggacggcaaa
     9241 attgtcgccc tgcgcgagaa caagcagcat ccggacgcca cgctgccgca ctatgacgct
     9301 ggcggtaagt tgatgctacc caccactcgc gacatgcata ttcatctcga caaaaccttc
     9361 tacggcgggc cgtggcgctc gctcaatcgt ccggcaggca ccactatcca ggacatgatc
     9421 aaactcgagc agaaaatgct gccggagtta caaccgtata cgcaggagcg ggcggaaaaa
     9481 ctgatcgatc tattgcagtc gaaaggcacc accattgccc gcagccactg caatatcgaa
     9541 ccggtttccg gcctaaaaaa tctgcaaaat ttgcaggcgg tgctggcgcg gcgtcaggcg
     9601 ggcttcgagt gtgaaatcgt cgccttcccg cagcacggtt tgctgctgtc gaaatcggaa
     9661 cccttaatgc gcgaagcgat gcaggcgggt gcgcattacg tcggcgggct ggacccgacc
     9721 agtgttgatg gcgcgatgga aaaatccctc gacaccatgt ttcagattgc gctggactac
     9781 gacaaaggcg tcgatattca cctgcatgaa accactccgg caggcgtggc agccatcaat
     9841 tatatggttg aaacggtaga gaaaacgcca cagctgaagg gcaagctgac catcagtcac
     9901 gcctttgcgc tggcaacgct caacgaacaa caggtagatg aactggcgca ccggatggcg
     9961 gcgcagcaaa tttctatcgc ctcgacggtg ccgattggca cgctgcatat gccgctcaaa
    10021 cagttgcacg acaaaggcgt aaaagtgatg actggcactg acagcgttat cgaccactgg
    10081 tcgccttacg gcctgggcga catgctggaa aaagccaatc tgtacgcgca gctctatatt
    10141 cgtcctaacg aacagaacct ctcccgctcg ctgtttttag ccactggcga tgtattgccg
    10201 ctcaacgaaa aaggcgagcg cgtgtggccc aaagcgcagg atgacgccag ttttgtgctg
    10261 gtggacgcct cctgttccgc cgaggcggtg gcgcgtatct cgccgagaac cgcaacgttc
    10321 cataaagggc aactggtgtg ggggagtgtg gcaggttgat gcgcatttga aacgcctgca
    10381 acggtgaata gtaagagatt taagccccag ggagtggggc aacctgaata tgagtgtgtc
    10441 ggcagaaatg gatattatct ttgaggaata tgcccttatt gacgttgtgt agataactat
    10501 ttgtctacgt gaaaagatca tcagttttgc cgattttagt cttttacgga tagcaaatat
    10561 cacacttaca ggtgctcgcc ttatggggaa cgttgctgct ttgtttcatt caggctcgcg
    10621 gcatgttggg gctggatcgg gtggtttaaa tattggctat attcaatgga cgcgttttgc
    10681 cgcgatgaca tatcaggcat cgccaaatac acatagctaa tcaggagtaa acacaatgaa
    10741 gatcaaagct gttggtgcat attccgctaa acaaccactt gaaccgatgg atatcacccg
    10801 gcgtgaaccg ggaccgaatg atgtcaaaat cgaaatcgct tactgtggcg tctgccattc
    10861 cgatctccac caggtccgtt ccgagtgggc ggggacggtt tacccctgcg taccgggtca
    10921 tgaaattgtg gggcgtgtgg tagccgttgg tgatcaggta gaaaaacatg cgccgggcga
    10981 tctggtcggt gtcggctgca ttgtcgacag ttgtaaacat tgcgaagagt gtgaagacgg
    11041 gctggaaaac tactgtgatc acatgaccgg cacctataac tcgccgacgc cggacgaacc
    11101 gggccatact ctgggcggct actcacaaca gatcgtcgtt catgagcgat atgttctgcg
    11161 tattcgtcac ccgcaagagc agctggcggc ggtggctcct ttgttgtgtg cagggatcac
    11221 cacgtattcg ccgctacgtc actggcaggc cgggccgggt aaaaaagtgg gcgtggtcgg
    11281 catcggcggt ctgggacata tggggatcaa gctggcccac gcgatggggg cgcatgtggt
    11341 ggcatttacc acttctgagg caaaacgcga agcggcaaaa gccctggggg ccgatgaagt
    11401 tgttaactca cgcaatgccg atgagatggc ggctcatctg aagagtttcg atttcatttt
    11461 gaatacagta gctgcgccac ataatctcga cgattttacc accttgctga agcgtgatgg
    11521 caccatgacg ctggttggtg cgcctgcgac accgcataaa tcgccggaag ttttcaacct
    11581 gatcatgaaa cgccgtgcga tagccggttc tatgattggc ggcattccag aaactcagga
    11641 gatgctcgat ttttgcgccg aacatggcat cgtggctgat atagagatga ttcgggccga
    11701 tcaaattaat gaagcctatg agcgaatgct gcgtggtgat gtgaaatatc gttttgttat
    11761 cgataatcgc acactaacag actgaaaaaa ttaataaata cgctgtggtt taacatatta
    11821 acttcgctct ccacttaact ttttagttaa ggagagcgaa ataatatcaa agtagcagta
    11881 aaacctataa cgtaaattca aattgttaaa ttaactccct ccagtacaca atacttcaca
    11941 cgttagttat gagcgatttc tgatagtgcc tggtttaatc agagctttat tatctgcgac
    12001 gtttattttt atttaagaga gatcgcgatg atatcattaa aagctccgca caataatttg
    12061 atgccatata cgcaacaaag catacttaat acggttaaaa acaatcagtt accagaggat
    12121 atcaaaagct ccctggtttc ctgtgtcgat atattcaagg ttttgattaa acaatattat
    12181 gattatcctt atgattgtcg tgatgattta gtcgatgacg acaaactcat tcatctcatg
    12241 gctgccgtac gagactgtga gtggtcagac gataacgcac tcaccataaa tgtgcagttt
    12301 aatgatttcc ctggatttta tgactggatg gattaccctg atcacccggt taagtttgtt
    12361 tttcgcatac ttgaaaatca aaaagggaca gtttgggtat atgaccagga tgatgcgttt
    12421 cttgatatta aagcaaatgt gcaggctgga cgctttaccg ggcttaagaa attagttcag
    12481 tttatcgatt cagtgcgaac agattgcaaa tgtattcttc ttgaacacca tatgcctttg
    12541 ctgagacttt tccctaaagg gaaagagtgt atgcatgtgg aaaaatggct tcgcgaaatg
    12601 tcgtctattc cggagacaga cgcgcccatt aagcaggcgc ttgcgcatgg attattatta
    12661 cacttaaaaa atatctaccc tgtttttcct gaatcactgg tgatgttatt gctctctgtt
    12721 ctggacgtaa aaacatacag agatgacgca cgattaaatg aatggatatc gaatagagtg
    12781 caggaattag gtgacagata ttatcctgta aataagcacg ttaaaatccg ctacactctt
    12841 tagttcagaa agggttccac ctgcaagttg ttattccacc agagagtgaa tcgcaaagca
    12901 ggtaatcatt tcatctgtat atccgtatgc taaatggcat tccaccgcct tttcgccgat
    12961 attttgttgt gatcagcgac gcttcgctta aaaaagcacc agcagtggtt tcgcagctat
    13021 gtggtgtata aaaaatgatc tcatgcagat gttttgtgaa tgtgtgggtt gacattcata
    13081 tgaaaaaaat cataattcca tcatgtttgt atggggtctt ttctgtacct tacgcatcgc
    13141 actcaagcct gacagaaaat atgctgtaag gctcatatca aaaaccgcca ttagctcatc
    13201 aggaagagca gacgacaacc aggattgttg tatggtacgg ggttcgaggc ctcgatggcg
    13261 gtccaacttt tcaaaactct tctgaatcag atccccttat tatcatccct gcaaagtgga
    13321 acattgccat tgtttggcta ttcaggacgg gtcagaccaa tgaaaaaagc ccacacaggg
    13381 gagagtgggc tgaaatggga agctaaagac tcaagtaaac ttatcggaaa taaggaccac
    13441 gcattacggg ggctatcatc gccgatggtc gggattcggc aaatcaggtt gattacgtag
    13501 agagtaaatt atctgctcac cgctgcgtca ccccttcgta aatcaggcgt agcgcgaata
    13561 caccaataat tgcaccaata acccgactgg caacgcgttg catacgccca taagcacgac
    13621 gcacagcggg caaagaaaac gcctgactaa gaaaaactcg ccagataatt gatgcgagca
    13681 caatccccgc ccaggccatt aaacgtgccc atgttggtgt ttcggcattt aatgttactg
    13741 agaaaatact gataaaaaat aaaacggttt gcgggttaga gagatcggta attaatccgc
    13801 ggcgaaaaaa gacataccag ggggcgctaa tcggttgttg tagtgtgctc atttgcggtg
    13861 ttgactggcg gcgcatgctg caccacgcaa accataagag ataagcgccg ccgacgattc
    13921 tgataagcga aaaaatctcc tcacactgcg taattagcgt tgcaagacca aacaaaccca
    13981 acccggaata aaatgcatcg cccagcgcca cgcccagccc ggtcagcacc cctgcgcgtc
    14041 gaccggaagc caggctggtt tgtactacca caaagagatt ggctcccgga ttaaaaaaag
    14101 taatcacgaa cagtcctacg gtcaggtaaa cggcgtgcaa aggatccata gtgatttcat
    14161 ccataaataa gtgaagtaac ttcatcatat ttctaccaaa aataatcggg tgcgagagag
    14221 atcacaaagt gtcttatttc cggttactgg cgtttatgcc ctgactgaac taattattaa
    14281 tcaacccaat aatgtgggtg gatgatagtg tgataacaac tctggagccg taatatgaaa
    14341 ataatctcta aaatgttagt cggtgcgtta gcgttagccg ttaccaatgt ctatgccgct
    14401 gaattgatga ccaaagcgga atttgaaaaa gttgaatcgc agtatgaaaa aataggtgat
    14461 atttcaacca gcaatgaaat gtcgactgca gatgcaaaag aagatttgat caaaaaagcg
    14521 gatgaaaaag gggctgatgt gttggtactg acctccggtc aaactgacaa taagatccac
    14581 ggcacggcaa atatttataa gaagaagtaa ttctgaaccc tatgtaaaca tgcccgatgc
    14641 gtaagtttat cggtgatcat ctattgaaat ttatgccgga taaagcgttc gcgctgcatt
    14701 cggcagttca gcttttcagc cgccgccaga acgtcgtccg gctgatgcct aaataattcg
    14761 ccgctgctgt tttatcgcca ttaaatttct ccagtgcctg ttgtggtgtc agtaagcgtg
    14821 gagcgggagt tttcgccgac tcgcgcgcca gttccggcag tagcagctgc aaaaattgcg
    14881 gcgttaaatc cggcgtcggt tccacactta aaaatagcgc cagtcgctcc atcatattgc
    14941 gcagttcacg aatattacct ggccagtcgt agtgcaccag cacggtttcg cttgcctgta
    15001 atccctggcg taatgcggca gaaaacgggg cggagagcgc cgccagagac actttcaaaa
    15061 agctttccgc cagcggaaga atatccgcca cccgctcgcg cagtggtggc aattgcagac
    15121 gcaaaatact cagccgataa aacagatcac ggcgaaaacg tccttgctgc atatcttctt
    15181 ccagcttgca gtgagtggcg ctaatgaccc gtacatctac cggaacaggc tgatgcccgc
    15241 cgacgcgggt gacctctttt tcttccagca cccgtaacag gcgagtctgc aaaggtagcg
    15301 gcatttcgcc aatctcatcc agaaacagcg taccgccgtg ggcaatttcg aacagcccgg
    15361 cgcgacctcc gcgtcgcgag ccggtaaacg ccccttcctc atagccaaac agctccgctt
    15421 ccagcagcga ttcggctatc gccccgcagt tgacggcaac aaacggatgc gactttttgc
    15481 cctgtcgcgc atcgtggcgg gcaaaatatt cccgatgaat cgcctgggcc gccagctctt
    15541 tgcccgtccc cgtctccccc tcaatcaaca ccgctgcact ggagcgggca tacagcaaaa
    15601 tagtctgccg tacttgttcc atctgtggtg attgaccgag catatcgccc agcacgtaac
    15661 gagttctcag ggcgttgcgg gtggcatcgt gagtgttatg gcgtaacgac atgcgcgtca
    15721 tatccagcgc atcgctgaac gcctggcgca cggtggcggc ggaatagata aaaattccgg
    15781 tcattccggc ttcttctgcc agatcggtaa tcagccccgc gccgaccacc gcttcagtgc
    15841 cgttagcttt cagctcgtta atctgcccgc gggcgtcttc ttcggtaatg tagctacgtt
    15901 gatcgaggcg cagattaaag gttttttgaa acgccaccag cgctggaatg gtttcctgat
    15961 aggtgacaac gccgatagaa gaggtgagtt ttccggcttt tgccagtgcc tgtaacacat
    16021 cgtagccgct cggtttaatc aaaataactg gcactgacag gcggcttttc aggtacgcgc
    16081 cgttagagcc agcagcgatg atggcgtcac agcgttcgtt tgccagtttc ttgcggatgt
    16141 aggtcactgc tttttcaaag ccaagctgaa taggggtaat gttcgccagg tgatcaaact
    16201 cgaggctgat atcgcgaaac agctcgaaca ggcgcgttac agataccgtc cagataaccg
    16261 gtttgtcgtc attaagccgt ggtggatgtg ccatagcgca ccgcaaagtt aagaaaccga
    16321 atattgggtt tagtcttgtt tcataattgt tgcaatgaaa cgcggtgaaa cattgcctga
    16381 aacgttaact gaaacgcata tttgcggatt agttcatgac gttatctcta acaaattgaa
    16441 attaaacatt taattttatt aaggcaattg tggcacaccc cttgctttgt tttattaacg
    16501 aaaataacaa gttgataaca aaggatgggc tatgtctcta cactctccag gtaaagcgtt
    16561 tcgcgctgca ctgactaaag aaaatccatt gcagattgtt ggcaccatca acgctaatca
    16621 tgcgctgttg gcgcagcgtg ccggatatca ggcaatttat ctctccggcg gtggcgtggc
    16681 ggcagggtcg ctggggctgc ccgatctcgg tatttctacc cttgatgatg tgctgacaga
    16741 tattcgccgt atcaccgacg tttgttcgct gccactgctg gtggatgcgg atatcggttt
    16801 tggttcttcg gcctttaacg tggcgcgtac ggtgaaatcg atgattaaag ccggtgcggc
    16861 aggattgcat attgaagatc aggttggtgc gaaacgctgc ggtcatcgcc cgaacaaagc
    16921 gatcgtctcg aaagaggaga tggtggatcg gatccgcgcg gcggtggatg cgaaaaccga
    16981 tcctgatttt gtgatcatgg cgcgcaccga tgcgctggcg gtagaggggc tggatgcggc
    17041 gatcgagcgt gcacaggcct atgttgaagc gggtgccgag atgttgttcc cggaggcgat
    17101 taccgaactc gccatgtacc gccagtttgc cgatgcggtg caggtgccga tcctcgccaa
    17161 cattaccgaa tttggcgcaa caccgctgtt taccaccgac gaattacgca gcgcccatgt
    17221 cgcaatggcg ctgtacccac tttcagcgtt ccgcgccatg aaccgcgccg ctgagcatgt
    17281 ctacaacgtc ctgcgccagg aaggcacgca gaaaagcgtc atcgatacca tgcaaacccg
    17341 caacgagctg tacgaaagca tcaactacta ccagtacgaa gcgaagctcg acgacctgtt
    17401 tgcccgtagc caggtgaaat aaaacacaac gaaaaatatc tcgaccctac aaatgacgag
    17461 gacaacatga gcgacacaac gatcctgcaa aacagtaccc atgtcattaa accgaaaaaa
    17521 tctgtggcac tttctggcgt tccggcgggc aatacggcgc tctgcaccgt gggtaaaagc
    17581 ggcaatgacc tgcattaccg cggctacgat attcttgatc tggcggaaca ttgcgaattt
    17641 gaagaagtgg cgcatctgct gatccacggc aaactgccga cccgtgacga actcgccgct
    17701 tacaaaacga aactgaaagc cctgcgcggt ttaccggcta acgtgcgtac cgtgctggaa
    17761 gccttaccgg cggcgtcaca cccgatggat gttatgcgca ccggcgtttc cgcgctcggc
    17821 tgcacgctgc cagaaaaaga gggacatacc gtctctggcg cgcgggatat tgccgacaaa
    17881 ctactggcat cacttagctc gattctcctt tattggtatc actacagtca caacggcgaa
    17941 cgcatccagc cggaaaccga tgacgactcc atcggtggtc acttcctgca tctgctgcac
    18001 ggcgaaaagc catcgcaaag ctgggaaaag gcgatgcata tttcgctagt gctgtacgcc
    18061 gaacacgagt ttaacgcctc cacctttacc agtcgggtga ttgcgggcac tggctctgat
    18121 atgtattccg cgattattgg cgcgattggc gcactgcgcg ggccgaaaca cggcggagcg
    18181 aatgaagtgt cgctggagat ccagcaacgc tacgaaacgc cggacgaagc cgaagccgat
    18241 atccgcaagc gggtggaaaa caaagaagtg gtcattggtt ttggtcatcc ggtttatacc
    18301 atcgccgacc cgcgccacca ggtgatcaaa cgtgtggcga agcagctctc gcaggaaggc
    18361 ggctcgttga agatgtacaa catcgccgat cgcctggaaa cggtgatgtg ggagagcaaa
    18421 aagatgttcc ccaatctcga ctggttctct gctgtttcct acaacatgat gggcgttccc
    18481 accgagatgt tcacaccact gtttgtcatc gcccgtgtca ccggctgggc ggcgcacatc
    18541 attgaacaac gtcaggacaa caaaattatc cgtccttccg ccaattatgt tggaccggaa
    18601 gaccgccagt ttgtcgcgct ggataagcgc cagtaaacct ctacgaataa caataaggaa
    18661 acgtacccaa tgtcagctca aatcaacaac atccgcccgg aatttgatcg tgaaatcgtt
    18721 gatatcgtcg attacgtgat gaactacgaa atcagctcta aagtggccta cgacaccgca
    18781 cattactgcc tgctcgacac gctcggctgc ggtcttgaag ctctcgaata cccggcctgt
    18841 aaaaaactgc tggggccaat tgttcctggc accgtcgtac ccaacggcgt gcgcgtcccc
    18901 ggaactcagt ttcagctcga ccccgtccag gcggcattta acattggcgc gatgatccgc
    18961 tggctcgatt tcaacgatac ctggctggcg gcggagtggg ggcatccttc cgacaacctc
    19021 ggcggcattc tggcaacggc agactggctt tcgcgcaacg cggtcgccag cggcaaagcg
    19081 ccgttgacca tgaaacaggt gctgaccgca atgatcaaag cccatgaaat tcagggctgc
    19141 atcgcgctgg aaaactcctt taaccgcgtc ggcctcgacc acgttctgtt agtgaaagtg
    19201 gcttccaccg ccgtggtcgc cgaaatgctc ggcctgaccc gcgaggaaat tctcaacgcc
    19261 gtttcgctgg cgtgggtgga cggtcagtcg ctgcgcacct atcgccatgc gccgaacacc
    19321 ggcacgcgta aatcctgggc ggcgggcgat gccacttccc gcgcggtacg tctggcactg
    19381 atggcgaaaa cgggcgaaat gggttacccg tcagccctga ccgcgccggt atggggcttc
    19441 tacgacgtct cctttaaagg tgaatcgttc cgcttccagc gtccgtacgg ttcttacgtc
    19501 atggagaatg tgctgttcaa aatctccttc ccggcggagt tccactccca gacggcagtt
    19561 gaagcggcga tgacgctcta tgaacagatg caggcagcag gcaagacggc ggcggatatc
    19621 gaaaaagtga ccattcgcac ccacgaagcc tgtattcgca tcatcgacaa aaaggggccg
    19681 ctcaataacc cggctgaccg cgaccactgc attcagtaca tggtggcgat cccgctgtta
    19741 ttcgggcgct taacggcggc agattacgag gacaacgttg cgcaagataa acgcatcgac
    19801 gccctgcgcg agaagatcaa ttgctttgaa gatccggcat ttaccgctga ctaccacgac
    19861 ccggaaaaac gcgccatcgc caatgccatt acccttgagt tcaccgacgg cacacgattt
    19921 gaagaagtgg tggtggagta ccccattggt catgctcgcc gccgtcagga tggtattccg
    19981 aaactggtcg ataaattcaa aatcaatctc gcgcgccagt tcccgactcg ccaacagcag
    20041 cgcattctgg aggtttctct cgacagaact cgcctggaac agatgccggt caatgagtat
    20101 ctcgacctgt acgtcattta agtaaacggc ggtaaggcgt aagttcaaca ggagagcatt
    20161 atgtctttta gcgaatttta tcagcgttcg attaacgaac cggagcagtt ctgggccgag
    20221 caggcccggc gtattgactg gcagacgccc tttacgcaaa cgctcgacca cagcaatccg
    20281 ccgtttgccc gttggttttg tgaaggccga accaatttgt gccacaacgc tatcgaccgc
    20341 tggctggaga aacagccaga ggcactggca ttgattgccg tctcttcgga aacagaagaa
    20401 gagcgcacct ttacctttcg ccagctgcat gacgaagtga acgcggtggc ctcaatgttg
    20461 cgctcactgg gcgtgcagcg cggcgatcgg gtgctggtgt atatgccgat gattgccgaa
    20521 gcgcatatta ctctgctggc ctgcgcgcgc attggtgcta ttcattcggt ggtatttggt
    20581 ggttttgcct cgcacagcgt ggcggcgcga attgatgacg ctaaaccggt gctgattgtc
    20641 tcggctgatg ccggagcgcg cggtggcaaa atcattccct ataaaaaatt gctcgacgat
    20701 gcgataagtc aggcgcagca ccagccacgc catgttttgc tggtggatcg cgggctggcg
    20761 aaaatggcgc gcgtcagcgg gcgggatgtc gatttcgcgt cgttgcgcca tcaacacatc
    20821 ggcgcgcggg taccggtggc gtggctggaa tccaacgaaa cctcctgcat tctctacact
    20881 tccggcacga ccggcaaacc taaaggcgtg cagcgtgacg tcggcggata tgcggtggcg
    20941 ctggcgacct cgatggacac catttttggc ggcaaagcgg gcagcgtgtt cttttgcgca
    21001 tcggatatcg gctgggtggt ggggcattcg tatatcgttt acgcgccgct gctggcgggg
    21061 atggcgacta tcgtttacga aggattgccg acctggccgg actgcggcgt gtggtggaaa
    21121 atcgtcgaga aatatcaggt tagccggatg ttctcagcgc cgaccgccat tcgcgtgctg
    21181 aaaaaattcc ctaccgctga aattcgcaaa cacgatctct cgtcgctgga agtgctctat
    21241 ctggctggag aaccgctgga cgagccgacc gccagttggg tgagcaatac gctggatgtg
    21301 ccggtcatcg acaactactg gcagaccgaa tccggctggc cgattatggc gattgctcgc
    21361 ggtctggacg acaggccgac gcgtctggga agccccggcg tgccgatgta tggctataac
    21421 gtgcagttgc ttaatgaagt caccggcgaa ccgtgtggcg tcaacgagaa agggatgctg
    21481 gtggtggaag ggccgctgcc gccgggctgt attcagacca tctggggcga cgacgaccgc
    21541 tttgtgaaga cttactggtc gctgttttcc cgcccggtgt acgccacctt tgactggggc
    21601 atccgtgacg ctgacggtta tcactttatt ctcgggcgca ctgacgatgt aattaacgtt
    21661 gccgggcatc gtctgggcac gcgcgagatt gaagagagta tctccagcca tccgggcgtt
    21721 gccgaagtgg cggtggttgg ggtgaaagat gcgctgaaag ggcaggtggc ggtggcgttt
    21781 gtcattccga aagagagcga tagtctggaa gatcgtgatg tggcgcattc gcaagagaag
    21841 gcgattatgg cgctggtgga cagccagatt ggcaactttg gtcgcccggc gcacgtctgg
    21901 tttgtctcgc aattgccgaa aacgcgatcc ggaaaaatgc tgcgccgcac gatccaggca
    21961 atttgcgaag gacgcgatcc tggggatctg acgaccattg atgatcctgc gtcattggat
    22021 cagatccgcc aggcgatgga agagtaaggt attgtcggat gcgtcgcgtg gcgcatccga
    22081 caccatgcgc cgatgcctga tgcgacgctt accgcgtctt attatgccta caggcctgaa
    22141 ccgtaggtcg gataaggcgc tcgcgtcgca tccgacaccc cgcgccgatg cctgatgcga
    22201 cgcttaccgc gtcttattat gcctacccac tgtttttaca ccgataattt ttcccccacc
    22261 tttttgcact cattcatata aaaaatatat ttccccacga aaacgattgc tttttatctt
    22321 cagatgaata gaatgcggcg gattttttgg gtttcaaaca gcaaaaaggg ggaatttcgt
    22381 gtcgcaagat aacaacttta gccaggggcc agtcccgcag tcggcgcgga aaggggtatt
    22441 ggcattgacg ttcgtcatgc tgggattaac cttcttttcc gccagtatgt ggaccggcgg
    22501 cactctcgga accggtctta gctatcatga tttcttcctc gcagttctca tcggtaatct
    22561 tctcctcggt atttacactt catttctcgg ttacattggc gcaaaaaccg gcctgaccac
    22621 tcatcttctt gctcgcttct cgtttggtgt taaaggctca tggctgcctt cactgctact
    22681 gggcggaact caggttggct ggtttggcgt tggcgtggcg atgtttgcta ttccggtggg
    22741 caaggcaacc gggctggata ttaatttgct gattgccgtt tccggtttac tgatgaccgt
    22801 caccgtcttc tttggcattt cggcgctgac agttctttcg gtgattgcgg ttccggctat
    22861 tgcctgcctt ggcggttatt ccgtgtggct ggccgttaac ggcatgggcg gcctggacgc
    22921 cttaaaagcg gtcgttcccg cacaaccgtt agatttcaat gtcgcgctgg cgctggttgt
    22981 ggggtcattt atcagtgcgg gtacgctcac cgctgacttt gtccggtttg gtcgcaatgc
    23041 caaactggcg gtgctggtgg cgatggtggc ctttttcctc ggcaactcgt tgatgtttat
    23101 tttcggtgca gcgggcgctg cggcactggg gatggcagat atctctgatg tgatgattgc
    23161 tcagggactg ctgctgcccg cgattgtggt gctggggctg aatatctgga ccaccaacga
    23221 taacgcactc tatgcgtcgg gtttaggttt cgccaacatt accggtatgt cgagcaaaac
    23281 cctttcggta atcaacggta ttatcggtac ggtctgcgca ttatggctgt ataacaattt
    23341 tgtcggctgg ttgaccttcc tttcggcagc tattcctccg gtagggggcg tgatcatcgc
    23401 cgactatctg atgaaccgtc gccgctatga gcactttgcg accacgcgta tgatgagtgt
    23461 caattgggtg gcgattctgg cggtcgcgct ggggattgcc gcaggccact ggttaccggg
    23521 aattgttccg gtcaacgcgg tattaggtgg cgcgctgagc tatctgatcc ttaacccgat
    23581 tttgaatcgt aaaacgacag cagcaatgac gcatgtggag gttaacagtg tcgaataacg
    23641 ctttacaaac aattattaac gcccggttac caggcaaaga ggggctgtgg cagattcatc
    23701 tgcaggacgg aaaaatcagc gccattgatg cgcaatccgg cgtgatgccc ataactgaaa
    23761 acagcctgga tgccgaacaa ggtttagtta taccgccgtt tgtggagccg catattcacc
    23821 tggacaccac gcaaaccgcc ggacaaccga actggaatca gtccggcacg ctgtttgaag
    23881 gcattgaacg ctgggccgag cgcaaagcgt tattaaccca tgacgatgtg aaacaacgcg
    23941 cctggcaaac gctgaaatgg cagattgcca acggcattca gcatgtgcgt acccatgtcg
    24001 atgtttcgga tgcaacgcta actgcgctga aagcaatgct ggaagtgaag caggaagtcg
    24061 cgccgtggat tgatctgcaa attgtcgcct tccctcagga agggattttg tcgtatccca
    24121 acggtgaagc gttgctggaa gaggcgttac gcttaggggc agatgtagtg ggggcgattc
    24181 cgcattttga atttacccgt gaatacggcg tggagtcgct gcataaaacc ttcgccctgg
    24241 cgcaaaaata cgaccgtctc atcgacgttc actgtgatga gatcgatgac gagcagtcgc
    24301 gctttgtcga aaccgttgct gccctggcgc accgtgaagg catgggcgcg cgagtcaccg
    24361 ccagccacac cacggcaatg cactcctata acggggcgta tacctcacgc ctgttccgct
    24421 tgctgaaaat gtccggtatt aactttgtcg ccaacccgct ggtcaatatt catctgcaag
    24481 gacgtttcga tacgtatcca aaacgtcgcg gcatcacgcg cgttaaagag atgctggagt
    24541 ccggcattaa cgtctgcttt ggtcacgatg atgtcttcga tccgtggtat ccgctgggaa
    24601 cggcgaatat gctgcaagtg ctgcatatgg ggctgcatgt ttgccagttg atgggctacg
    24661 ggcagattaa cgatggcctg aatttaatca cccaccacag tgccagaaca ttgaatttgc
    24721 aggattacgg cattgccgcc ggaaacagcg caaacctgat tatcctgccg gctgaaaatg
    24781 gatttgatgc gctgcgccgt caggttccgg tacgttattc ggtacgtggc ggcaaggtga
    24841 ttgccagtac acaaccggca caaaccaccg tatatctgga gcagccagaa gccatcgatt
    24901 acaaacggta atttcctctg tgttgaagag ccactgacct gtgtcagtgg ctaccgtgat
    24961 tcattctcgc caacatccgc acattcctcc aacgccatgt gcaaaaatgc cttcgcggcg
    25021 gctgtctgcc agctattttt ccgccgcaac aaaaccgccg ttctctccag tagtggcggg
    25081 gcaagagaaa tcgctttaag cccgtcatgt tgtgtggcaa tcgctgctgg taacaatgtg
    25141 gaaagggaag tgcggcgaat cagctccaga accgcgctaa ttgagttcgc ctcaatgacc
    25201 acctgtggat gtagccccgc tttctcgcag tagtggtcaa tttgctctct ggtggcaaat
    25261 tccgcgctga gcaggaccag tttttcatca tgcaagcgac tcaacgccac ctgttcacag
    25321 gcagccagcg gatgatgttg cgccacgact aacgctaaac tttctgtcag taaaggaatt
    25381 gcctccagct ccggcgaatg cacaggcgcg aaggcaatcc caacgtccag ctcgtcgcga
    25441 caaagcagat cttcgatttt ctcctgcgac atttcctgta gctggagcgt gatgccgggg
    25501 tagcgcgcat agaaatccgc cattaaaggg ccgataaagt agcttgtaaa ggtgggggtg
    25561 acggcgatac gcagcgatcc tcgcgtcaga tcggcaacat catgaatcgc ccgtttaccc
    25621 gctcccagtt cctgtaacgc ccggctggcg tactgtcgcc agacttctcc tgcatcggtg
    25681 agacgaatcg ttcgcccgct acggtcaaac agcggcacgc ctaaactctc ctctaactgg
    25741 cgaatctgct gggaaagcgc aggctgagag acgtgcaacg cactggcggc acgggtgaag
    25801 ctgccatgtt cagccacggc aagaaaataa ttgatatgtc gagagagcat tcgccaacct
    25861 ataagtaaat ccaatagaac tcatcataaa tgagactttt accttatgac aatcggcgag
    25921 tagtctgcct ctcattccag agacagacag aggttaacgg tgaaagagat tattgatgga
    25981 ttccttaaat tccagcgcga ggcatttccg aagcgggaag ccttgtttaa acagctggcg
    26041 acacagcaaa gcccgcgcac actttttatc tcctgctccg acagccgtct ggtccctgag
    26101 ctggtgacgc aacgtgagcc tggcgatctg ttcgttattc gcaacgcggg caatatcgtc
    26161 ccttcctacg ggccggaacc cggtggcgtt tctgcttcgg tggagtatgc cgtcgctgcg
    26221 cttcgggtat ctgacattgt gatttgcggt cattccaact gtggcgcgat gaccgccatt
    26281 gccagctgtc agtgcatgga ccatatgcct gccgtctccc actggctgcg ttatgccgat
    26341 tcagcccgcg tcgttaatga ggcgcgcccg cattccgatt taccgtcaaa agctgcggcg
    26401 atggtacgtg aaaacgtcat tgctcagttg gctaatttgc aaactcatcc atcggtgcgc
    26461 ctggcgctcg aagaggggcg gatcgccctg cacggctggg tctacgacat tgaaagcggc
    26521 agcatcgcag cgtttgacgg cgcaacccgc cagtttgtgc cactggcagc taatccgcgc
    26581 gtttgtgcca taccgctacg ccaaccgacc gcagcgtaac cttattttta aaccatcagg
    26641 agatgcacca tgattcagtc gcaaattaac cgcaatattc gtcttgatct tgccgatgcc
    26701 attttgctca gcaaagctaa aaaagatctc tcatttgccg agattgccga cggtaccggt
    26761 ctggcagaag ccttcgtaac cgcggctttg ctggggcagc aggcgcttcc tgccgacgcc
    26821 gcccgcctgg tcggagcgaa gctggatctc gacgaagacg ccattctact gttgcagatg
    26881 attccactgc gtggctgcat tgatgaccgt attccaaccg acccaacgat gtatcgtttc
    26941 tatgaaatgt tgcaggtgta cggcacaacc ctgaaagcgt tggttcatga gaagtttggc
    27001 gatggcatta tcagcgcgat taacttcaaa ctcgacgtta agaaagtggc ggacccggaa
    27061 ggtggcgaac gtgcggtcat caccttagat ggtaaatatc tgccgaccaa accgttctga
    27121 cagccatgcg caaccatcaa aagacgttca cgatactgct ggttctggtg ctgattggtc
    27181 ttaatatgcg accactgctc acctccgtcg ggccactgct accgcaattg cgccaggcga
    27241 gcggaatgag ctttagcgtg gctgccctgt tgaccgctct gccggtggtt accatgggcg
    27301 ggctggcgct ggccggaagc tggcttcatc agcatgtcag cgaacgtcgc agcgtcgcca
    27361 tcagtttgtt gctgattgcc gtcggtgcat tgatgcgtga gctttacccc caaagtgcgc
    27421 tgctgcttag cagcgcactg ctcggtgggg tggggatcgg catcatccag gcggtgatgc
    27481 cttcggtgat taaacggcgg tttcagcagc gcacgccact ggtgatgggg ctatggtccg
    27541 cggctctgat gggcggcggt gggcttggtg ccgccctgac accctggtta gttcaacata
    27601 gcgaaacctg gtatcaaaca ctcgcctggt gggcgctgcc tgccgttgtt gcgctctttg
    27661 cctggtggtg gcaaagcacc cgcgaggtcg cctcttccca caagacaaca accactccgg
    27721 ttcgcgtggt tttcactcct cgcgcgtgga cgctgggtgt ttactttggt ctgattaacg
    27781 gtggttacgc cagcctgatt gcctggttac ccgccttcta tattgagatt ggtgccagcg
    27841 cgcagtacag cggttccttg ctggcattga tgacgcttgg gcaagccgca ggagctttgc
    27901 tgatgcctgc tatggctcgc catcaggatc ggcgcaaact gttaatgctg gcgctggtgt
    27961 tacaactggt ggggttctgc ggctttatct ggttgccgct gcaattgccg gtcttgtggg
    28021 cgatggtgtg tgggttaggt ctgggcggcg cgtttccgct ctgtttgctg ctggcgctcg
    28081 atcactctgt gcaaccggct attgctggca agctggtggc gtttatgcag ggaatcggtt
    28141 ttatcatcgc cgggcttgcc ccgtggtttt ctggcgtgct gcgtagtatc agcggcaatt
    28201 acctgatgga ctgggcattt catgcgctgt gcgtcgttgg gctgatgatt ataaccctgc
    28261 gttttgcacc agcacgtttt ccgcagctgt gggtcaaaga ggcatgatgc gacgcttgtt
    28321 cctgcgcttt gttcatgccg gatacggcta atgtagatcg ctgaacttgt aggcctgata
    28381 agcgcagcgt atcaggcaat ttttataatt taaactgacg attcaacttt ataatctttg
    28441 aaataatatt gcttatcccg gtcgtttatt tcgcgaataa cccgacaagg aacgccagcc
    28501 gccacgacgt ttggtggaat gtcttttgtg acgatactac ccgcgccaat aacagaatta
    28561 tccccgatgg tgacgcctgg attaataacc acatgacttc cgatccagac gttattgcca
    28621 atcgttatcg gaaaagagta catctcgccg ttttttctca attcatggtg tacagggtgt
    28681 cccgtaacgg aaagagtaac gttgggtgca atcagtacgt tatcaccgat tgttaccgtg
    28741 tagtcatcga caatggttaa attgaaattt gcataaaaat tgcggcctat atggatgttg
    28801 gaaccgtaag agaaatagac aggtggttct acccaggcgt tttcccctac cgtggcaaac
    28861 atttctttaa gcaggctttc ccttttttca acttctgatg gatgcgagtg attaaactca
    28921 tacattaacg ttttcccacg aagtcttttt tcaggtaagc cttcgcacat atcggtaaat
    28981 agcttgcctg cttttattct ttctgtcatc gacatgttca ttgcgatcat tccgtcctga
    29041 tatgttggtc ggataaggcg ctcgcgccgc atccgacatt gattgcttaa gcgacttcat
    29101 tcacctgacg acgcagtaga gaaagcgggc cggggccgct aagcgtgaac acggaaatta
    29161 aggtgaagcc cagcgccacc agacccagca ccagataagc gccctggaaa ccgatgcttt
    29221 catacatatt gcccgccagt acagacataa aaatcatcgc cagttgctta aagaagcaga
    29281 aacagaccag ataaatcgtc gctgaaaaac gcacttcaaa ctggctggta atatatttaa
    29341 agcagcccac cagcaggaac ggtacttcaa acatatgcag cgttttcaga ataactactt
    29401 ccagcgctga ggtggcgaac gatgagccaa taatacgtac agacataata gtgccagcca
    29461 gcagcagggc atttttccca ccgatgcgat taatgatcag tggcgcaaag aacataatcg
    29521 aggcgttaag taattcgccc attgtcgtta cgtagccaaa tacccgcgta ccctgttcac
    29581 cggtagcaaa gaacgaagta aagaaattag caaactgttg gtcaaaaaca tcgtaggtgc
    29641 aggaaacgcc aataacatac agtgacaaaa accacagttt tggctgtctg aacagttcca
    29701 gcgccagctt aaggctaaat gccgaatggt tggcacctac cgcattggca accgtggcag
    29761 aagagggcgc atccgttttg gcgaaaaaga gtaaaacggc gaggatgagt gcacagccag
    29821 agcccagcca gaaaacaaac tgattattga cggtgaacat gatgccgaca atcgaggcac
    29881 acagcgccca gccaacacag ccaaacatcc gcgcgcgacc aaattcgaaa ttactgcgac
    29941 ggctgacttt ctcaataaat gcctctactg ctggcgcacc ggcgttaaaa caaaagccta
    30001 gataaatacc accaacaatc gatcctacta aaatgttgta ttgtaacagt ggcccgaaga
    30061 taaaaataaa gaacggcgca aacatcacta acatgccggt aataatccac agcaggtatt
    30121 tgcgcagccc gagtttgtca gaaagcagac caaacagcgg ttggaataat agcgagaaca
    30181 gagaaatagc ggcaaaaata atacccgtat cacttttgct gatatggttg atgtcatgta
    30241 gccaaatcgg gaaaaacggg aagtaggctc ccatgataaa aaagtaaaag aaaaagaata
    30301 aaccgaacat ccaaaagttt gtgtttttta aatagtacat aatggatttc cttacgcgaa
    30361 atacgggcag acatggcctg cccggttatt attatttttg acaccagacc aactggtaat
    30421 ggtagcgacc ggcgctcagc tggaattccg ccgacactga cgggctccag gagtcgtcgc
    30481 caccaatccc catatggaaa ccgtcgatat tcagccatgt gccttcttcc gcgtgcagca
    30541 gatggcgatg gctggtttcc atcagttgct gttgactgta gcggctgatg ttgaactgga
    30601 agtcgccgcg ccactggtgt gggccataat tcaattcgcg cgtcccgcag cgcagaccgt
    30661 tttcgctcgg gaagacgtac ggggtataca tgtctgacaa tggcagatcc cagcggtcaa
    30721 aacaggccgc agtaaggcgg tcgggatagt tttcttgcgg ccctaatccg agccagttta
    30781 cccgctctgc gacctgcgcc agctggcagg tcaggccaat ccgtgccgga tgcggtgtat
    30841 cgctcgctac ttcaacatca acggtaatcg ccatttgacc actaccatca atccggtagg
    30901 ttttccggct gataaataag gtttttccct gatgctgcca cgcgtgagcg gtcgtaatca
    30961 gcaccgcgtc ggcaagtgta tctgccgagc actgcaacaa cgctgcttcg gcctggtaat
    31021 ggcccgccgc cttccagcgt tcgacccagg cgttagggtc aatgcgggtc gcttcactta
    31081 cgccaatgtc gttatccagc ggtgcacggg tgaactgatc gcgcagcggc gtcagcagtt
    31141 gtttttcatc gccaatccac atctgtgaaa gaaggcctga ctgacggtta aattgccaac
    31201 gcttattacc cagctcgatg caaaaatccg tttcgctggt ggttagttgc gggatgatat
    31261 gggacgcgga ggggagcgtc acactgaggt tttccgccag acgccactgc tgccaggcgc
    31321 tgatgtgccc ggcttctgac catgcggtcg cgttcggttg cactacgcgt actgtgagcc
    31381 agagttgccc ggcgctctcc ggctgcggta gttcaggcag ttcaatcaac tgtttacctt
    31441 gtggagcgac atccagaggc acttcaccgc ttgccagcgg cttaccatcc agcgccacca
    31501 tccagtgcag gagctcgtta tcgctatgac ggaacaggta ttcgctggtc acttcgatgg
    31561 tttgcccgga taaacggaac tggaaaaact gctgctggtg ttttgcttcc gtcagcgctg
    31621 gatgcggcgt gcggtcggca aagaccagac cgttcataca gaactggcga tcgttcggcg
    31681 tatcgccaaa atcaccgccg taagccgacc acgggttgcc gttttcatca tatttaatca
    31741 gcgactgatc cacccagtcc cagacgaagc cgccctgtaa acggggatac tgacgaaacg
    31801 cctgccagta tttagcgaaa ccgccaagac tgttacccat cgcgtgggcg tattcgcaaa
    31861 ggatcagcgg gcgcgtctct ccaggtagcg aaagccattt tttgatggac catttcggca
    31921 cagccgggaa gggctggtct tcatccacgc gcgcgtacat cgggcaaata atatcggtgg
    31981 ccgtggtgtc ggctccgccg ccttcatact gcaccgggcg ggaaggatcg acagatttga
    32041 tccagcgata cagcgcgtcg tgattagcgc cgtggcctga ttcattcccc agcgaccaga
    32101 tgatcacact cgggtgatta cgatcgcgct gcaccattcg cgttacgcgt tcgctcatcg
    32161 ccggtagcca gcgcggatca tcggtcagac gattcattgg caccatgccg tgggtttcaa
    32221 tattggcttc atccaccaca tacaggccgt agcggtcgca cagcgtgtac cacagcggat
    32281 ggttcggata atgcgaacag cgcacggcgt taaagttgtt ctgcttcatc agcaggatat
    32341 cctgcaccat cgtctgctca tccatgacct gaccatgcag aggatgatgc tcgtgacggt
    32401 taacgcctcg aatcagcaac ggcttgccgt tcagcagcag cagaccattt tcaatccgca
    32461 cctcgcggaa accgacatcg caggcttctg cttcaatcag cgtgccgtcg gcggtgtgca
    32521 gttcaaccac cgcacgatag agattcggga tttcggcgct ccacagtttc gggttttcga
    32581 cgttcagacg tagtgtgacg cgatcggcat aactaccacg ctcatcgata atttcaccgc
    32641 cgaaaggcgc ggtgccgctg gcgacctgcg tttcaccctg ccataaagaa actgttaccc
    32701 gtaggtagtc acgcaactcg ccgcacatct gaacttcagc ctccagtaca gcgcggctga
    32761 aatcatcatt aaagcgagtg gcaacatgga aatcgctgat ttgtgtagtc ggtttatgca
    32821 gcaacgagac gtcacggaaa atgccgctca tccgccacat atcctgatct tccagataac
    32881 tgccgtcact ccagcgcagc accatcaccg cgaggcggtt ttctccggcg cgtaaaaatg
    32941 cgctcaggtc aaattcagac ggcaaacgac tgtcctgacc gtaaccgacc cagcgaccgt
    33001 tgcaccacag atgaaacgcc gagttaacgc catcaaaaat aattcgcgtc tggccttcct
    33061 gtagccagct ttcatcaaca ttaaatgtga gcgagtaaca acccgtcgga ttctccgtgg
    33121 gaacaaacgg cggattgacc gtaatgggat aggtcacgtt ggtgtagatg ggcgcatcgt
    33181 aaccgtgcat ctgccagttt gaggggacga cgacagtatc ggcctcagga agatcgcact
    33241 ccagccagct ttccggcacc gcttctggtg ccggaaacca ggcaaagcgc cattcgccat
    33301 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ttcttcgcta ttacgccagc
    33361 tggcgaaagg gggatgtgcc gcaaggcgat taagttgtgt aacgccaggg ttttcccagt
    33421 cacgacgttg taaaacgacg gccagtgaat ccgtaatcat ggtcatagct gtttcctgtg
    33481 tgaaattgtt atccgctcac aatttcacac aacatacgag ccggaagcat aaagtgtaaa
    33541 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    33601 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    33661 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
    33721 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
    33781 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
    33841 ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt
    33901 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
    33961 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
    34021 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
    34081 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
    34141 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
    34201 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
    34261 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
    34321 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
    34381 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
    34441 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
    34501 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
    34561 tttttctcgc gttttcgcag aaacgtggct ggcctggttc accacgcgag aaacggtctg
    34621 ataagagaca ccggcatact ctgcgacatc gtatagcgtt actggtttca cattcaccac
    34681 cctgaattga ctctcttccg ggcgctatca tgccatacca cgaaaggttt tgcgccattc
    34741 gatggtgtca acgtaaatgc acgccgcttc gccgtccggc caccagaata gcctgcgatt
    34801 caaccccttc ttcgatctgt tttgctaccc gttgtagcgc cggaagatgc ttttccgctg
    34861 cctgttcaat ggtcattgcg ctcgccatat acaccagatt cagacagcca atcacccgtt
    34921 gttcactgcg cagcggtacg gcgatagagg caatcttctc ctcctgatcc cagccgcggt
    34981 agttctgtcc gtaaccctct ttgcgcgcgc gcgccagaat ggcttgcagc tttaacggtt
    35041 ctcgtgccag ttgatagtca tcaccggggc gggcggctaa catttcgatt aattccttgc
    35101 gttcttgttc cgggcaaaag gccagccagg tcaggcccga ggcggttttc aggagcggta
    35161 aacgtcgccc gaccattgcc cggtgaaagg ataagcggct gaaacggtgg gtggtttcgc
    35221 gtaccaccat tgcatcaaca tccagggtgg acacatctgt tggccatacc acttcgcgca
    35281 acagatcgcc cagcagcggg gccgccagtg cagaaatcca ctgttcgtca cgaaatcctt
    35341 cgcttaattg ccgcactttg atggtcagac gaaaactgtc atcggagggg ctacggcgga
    35401 catatccctc ttcctgcagc gtctccagca gtcgccgcac agtggtgcga tgcaggccgc
    35461 tgagttccgc cagcagcccg acgctggcac cgccatcaag tttatttaac atatttaata
    35521 acattagacc gcgggttaag ccgcgcacgg ttttgtattc cgtctgctca ttgttctgca
    35581 tattaattga catttctata gttaaaacaa cgttgtgcac ctggtgcaca ttcgggcatg
    35641 ttttgattgt agccgaaaac acccttccta tactgagcgc acaataaaaa atcatttaca
    35701 tgtttttaac aaaataagtt gcgccgtact gtgcgcgcaa cggcattttg tccgagtcgt
    35761 gaggtactga aatggcaata caacaccctg acatccagcc tgctgttaac catagcgttc
    35821 aggtggcgat cgctggtgcc ggtccggttg ggctgatgat ggcgaactat ctcggtcaga
    35881 tgggcattga cgtgctggtg gtggagaaac tcgataagtt gatcgactac ccgcgtgcga
    35941 ttggtattga tgacgaggcg ctgcgcacca tgcagtcggt tggcctggtc gagaatgttc
    36001 tgccgcacac tacgccgtgg cacgcgatgc gttttctcac cccgaaaggc cgctgttttg
    36061 ctgatattca gccaatgacc gatgaatttg gctggccgcg ccgtaacgcc tttattcagc
    36121 cgcaggtcga tgcggtgatg ctggaagggg tgtcgcgttt tcctaatgtg cgctgcttgt
    36181 tttcccgcga gctggaggcc ttcagccagc aaaatgacga agtgaccttg cacctgaaaa
    36241 cggcagaagg gcagcgggaa atagtcaaag cccagtggct ggtggcctgt gatggtgggg
    36301 caagttttgt tcgtcgcacc ctgaatgtgc cgtttgaagg taaaactgcg ccaaatcagt
    36361 ggattgtggt ggatatcgcc aacgatccgt taagtacgcc gcatatctat ttatgttgcg
    36421 atccggtgcg cccgtatgtt tctgccgcgc tgcctcatgc ggtacgtcgc tttgaattta
    36481 tggtgatgcc gggagaaaac gaagaacagc tgcgtgagcc gcaaaatatg cgcaagctgt
    36541 taagcaaagt gctgcctaat ccggacaatg ttgaattgat tcgccagcgt gtctacaccc
    36601 acaacgcgcg actggcgcaa cgtttccgta ttgatcgcgt actgctggcg ggcgatgccg
    36661 cgcacatcat gccggtatgg caggggcagg gctataacag tggtatgcgc gacgccttta
    36721 acctcgcatg gaaactggcg ttggttatcc aggggaaagc ccgcgatgcg ctgctcgata
    36781 cctatcaaca agaacgtcgc gatcacgcca aagcgatgat tgacctgtcc gtgacgacgg
    36841 gcaacgtgct ggctccgccg aaacgctggc agggtacgtt acgtgacggc gtttcctggc
    36901 tactgaatta tctgccgcca gtaaaacgct acttcctcga aatgcgcttc aagccgatgc
    36961 cgcaatatta cggcggtgcg ctggtacgtg agggcgaagc gaagcactct ccggtcggca
    37021 agatgtttat tcagccgaaa gtcacgctgg aaaacggcga cgtgacgctg ctcgataacg
    37081 cgattggcgc gaacttcgcg gtaattggct ggggatgcaa tccactgtgg gggatgagcg
    37141 acgagcaaat ccagcagtgg cgcgcgttgg gcacacgctt cattcaggtg gtgccggaag
    37201 tgcaaattca taccgcacag gataaccacg acggcgtgct acgcgtgggc gatacgcaag
    37261 gtcgtctgcg tagctggttc gcgcaacaca atgcttcgct ggtggtgatg cgcccggatc
    37321 gctttgttgc cgccaccgcc attccgcaaa ccctgggcaa tacgctgaat aaactggcgt
    37381 cggtgatgac gctgacccgc cctgatgccg acgtttctgt cgaaaaggta gcctgatatg
    37441 cacgcttatc ttcactgtct ttcccactcg ccgttggtgg ggtatgtcga cccggcgcaa
    37501 gaggtactcg atgaggtcaa tggcgtgatt gccagcgccc gcgagcgtat tgcggctttc
    37561 tcccctgaac tggtggtgct gtttgcgcca gatcactaca acggcttttt ctatgacgtg
    37621 atgccaccgt tctgtttagg cgttggagcg acggcaattg gtgatttcgg cagtgcggca
    37681 ggagagctgc ccgtgcctgt ggagctggcg gaggcctgtg cgcatgctgt catgaagagc
    37741 gggatcgatc ttgccgtttc ttactgtatg caggtggacc acgggttcgc ccagccgctg
    37801 gagttcctgc tcggtgggct ggataaggtg ccagttctgc ctgtgttcat caacggtgtc
    37861 gccacgccgc tgcccggttt ccagcgtacc cgcatgttgg gtgaagccat tggacgtttc
    37921 accagcactc tcaataaacg cgtgctgttc ctgggttccg gtgggctttc ccatcagccg
    37981 ccggtgcccg aactggcgaa agccgatgcc catatgcgcg accgtctgtt ggggagcggg
    38041 aaagatttac ccgccagtga gcgcgaattg cgtcagcaac gggtgattag cgccgctgag
    38101 aagtttgttg aggatcagag aactctgcat ccgctcaacc cgatttggga taaccagttc
    38161 atgactttgc tggagcaggg acgcatacag gaactggatg ccgtcagtaa cgaagagctt
    38221 tccgccattg ccggaaagtc gacacatgaa atcaaaacct gggtcgccgc ttttgccgct
    38281 atttctacat ttggcaactg gcgtagcgaa gggcgttatt accgcccaat cccggagtgg
    38341 attgccggat ttggctcgtt aagcgccaga acagagaact gaatatgcag gagaagatga
    38401 tgagttatca gccacaaacc gaagccgcca ccagccgttt tctgaatgta gaagaagcgg
    38461 gtaaaacgct gcgcatccat tttaatgact gcggacaagg cgacgaaacc gttgtcctgc
    38521 tgcatggttc cggcccgggt gctactggct gggcgaactt cagccgcaat atcgatccgc
    38581 tggtagaggc gggctatcgg gtaatcctgc tggattgtcc gggttggggc aaaagcgatt
    38641 cgatcgttaa tagtggttca cgatcggatc ttaatgcacg aatcctgaaa agcgtggtgg
    38701 atcaactgga tatcgccaaa atccacctgc tgggcaactc gatggggggc catagttctg
    38761 tggcgttcac ccttaaatgg ccggagcgcg tcggcaaact ggtgctgatg ggcggcggta
    38821 cgggcggcat gagtttgttt acgccgatgc caaccgaagg tattaagcga ctgaatcagc
    38881 tttatcgtca gccgactatc gaaaacctga agctgatgat ggatatcttc gtttttgata
    38941 ccagcgattt gaccgacgcc ctgtttgaag cgcgcctgaa taatatgctg tcgcgccgcg
    39001 atcacctgga aaacttcgtt aagagcctgg aagctaatcc gaaacagttc ccggattttg
    39061 gcccacgtct ggcggaaatc aaagcgcaaa ccctgattgt ctgggggcgc aacgaccgct
    39121 ttgtgccgat ggatgcgggt ctgcgtctgc tgtccggcat tgccggttct gaactgcata
    39181 tcttccgcga ctgtggtcac tgggcgcagt gggaacatgc cgacgctttc aatcaattgg
    39241 tgctgaattt cctcgcacgc ccttaaggaa tggtcatgac gaagcatact cttgagcaac
    39301 tggcggcgga tttacgccgc gccgcagagc agggcgaagc gattgcgccg ctgcgcgatc
    39361 tgattggtat cgataacgct gaagcggcct acgccattca gcacataaat gtgcaatatg
    39421 acgttgtgca ggggcgtcgc gtggtagggc gtaaagtggg cctgacgcat ccgaaagtgc
    39481 aacaacaact gggcgttgat caaccggatt ttgggacgtt atttgccgac atgtgttatg
    39541 gcgataacga aatcattcct ttttcccgtg ttctgcaacc ccgcattgaa gcggagatcg
    39601 cactggtgtt gaaccgcgat ttacccgcaa ctgatatcac cttcgacgaa ctgtataacg
    39661 ccattgaatg ggtacttccg gcgctggaag tggtggggag ccgcattcgc gactggtcga
    39721 ttcagtttgt cgataccgtg gcagataacg cttcctgcgg ggtgtatgtc atcggcggtc
    39781 cggcgcaacg tccggcgggg ttagacctga aaaactgcgc catgaagatg acgcgtaata
    39841 acgaagaggt ttccagcggg cgcggcagcg aatgcctggg acatccgctt aatgccgccg
    39901 tctggctggc acgcaaaatg gccagtctgg gtgaaccgct gcgcgccgga gatatcattc
    39961 ttaccggggc attaggcccg atggtggcgg tgaatgcggg cgatcgtttt gaagcccata
    40021 ttgaaggcat aggttcagtt gctgcgacat tttcaagcgc agccccaaaa ggaagtctgt
    40081 catgagtaag cgtaaagtcg ccattatcgg ttctggcaac attggcaccg atctgatgat
    40141 taaaattttg cgtcacggtc agcatctgga gatggcggtg atggtcggca ttgatcctaa
    40201 gtccgacggt ctggcgcgtg ccagacgtat gggcgtcgcc accacccatg aaggggtgat
    40261 cgggctgatg aacatgcctg aatttgctga tatcgacatt gtatttgatg cgaccagcgc
    40321 gggcgcgcat gtgaaaaacg atgccgcttt acgcgaagcg aaaccggata ttcgcttaat
    40381 tgacctgacg cctgctgcca tcggccctta ctgcgtgccg gtggttaacc tcgaggcgaa
    40441 cgtcgatcaa ctgaacgtca acatggtcac ctgcggcggc caggccacca ttccaatggt
    40501 ggcggcagtt tcacgcgtgg cgcgtgttca ttacgccgaa attatcgctt ctatcgccag
    40561 taaatctgcc ggacctggca cgcgtgccaa tatcgatgaa tttacggaaa ccacttcccg
    40621 agccattgaa gtggtgggcg gcgcggcaaa agggaaggcg attattgtgc ttaacccagc
    40681 agagccaccg ttgatgatgc gtgacacggt gtatgtattg agcgacgaag cttcacaaga
    40741 tgatatcgaa gcctcaatca atgaaatggc tgaggcggtg caggcttacg taccgggtta
    40801 tcgcctgaaa cagcgcgtgc agtttgaagt tatcccgcag gataaaccgg tcaatttacc
    40861 gggcgtgggg caattctccg gactgaaaac agcggtctgg ctggaagtcg aaggcgcagc
    40921 gcattatctg cctgcctatg cgggcaacct cgacattatg acttccagtg cgctggcgac
    40981 agcggaaaaa atggcccagt cactggcgcg caaggcagga gaagcggcat gaacggtaaa
    41041 aaactttata tctcggacgt cacgttgcgt gacggtatgc acgccattcg tcatcagtat
    41101 tcgctggaaa acgttcgcca gattgccaaa gcactggacg atgcccgcgt ggattcgatt
    41161 gaagtggccc atggcgacgg tttgcagggt tccagcttta actatggttt cggcgcacat
    41221 agcgaccttg aatggattga agcagcggcg gatgtggtga agcacgccaa aatcgcgacg
    41281 ttgttgctgc caggaatcgg cactattcac gatctgaaaa atgcctggca ggctggcgcg
    41341 cgggtggttc gtgtggcaac gcactgtacc gaagctgatg tttccgccca gcatattcag
    41401 tatgcccgcg agctcggaat ggacaccgtt ggttttctga tgatgagcca tatgaccacg
    41461 ccggagaatc tcgccaagca ggcaaagctg atggaaggct acggtgcgac ctgtatttat
    41521 gtggtggatt ctggcggtgc gatgaacatg agcgatatcc gtgaccgttt ccgcgccctg
    41581 aaagcagagc tgaaaccaga aacgcaaact ggcatgcacg ctcaccataa cctgagtctt
    41641 ggcgtggcga actctatcgc ggcggtggaa gagggctgcg accgaatcga cgccagcctc
    41701 gcgggaatgg gcgcgggcgc aggtaacgca ccgctggaag tgtttattgc cgccgcggat
    41761 aaactgggct ggcagcatgg gaccgatctc tatgcgttaa tggatgccgc cgacgacctg
    41821 gtgcgtccgt tgcaggatcg accggtacga gtcgatcgcg aaacgctggc gctgggatac
    41881 gctggtgttt actcgagctt cctgcgtcac tgtgaaacgg cggcggcgcg ttatggctta
    41941 agtgcggtgg atattctcgt tgagctgggc aaacgccgga tggttggcgg ccaggaggat
    42001 atgatcgtcg acgtggcgct ggatctgcgc aacaacaaat aatgatgact gccgataacg
    42061 tgcattttgt atgcccatcc cagcacgaca gccggatgcg gcgtgaacgc cttatccggc
    42121 ctacatttcg cccgtaggca gtcattaaat agttctgatt acgcggctgg caatgtatca
    42181 gtcgcgattc acctgcactc gcaatgaggt tcatcttatg tcgactcgta ccccttcatc
    42241 atcttcatcc cgcctgatgc tgaccatcgg gctttgtttt ttggtcgctc tgatggaagg
    42301 gctggatctt caggcggctg gcattgcggc gggtggcatc gcccaggctt tcgcactcga
    42361 taaaatgcaa atgggctgga tatttagcgc cggaatactc ggtttgctac ccggcgcgtt
    42421 ggttggcggg atgctggcag accgttatgg tcgtaagcgt attttgattg gctcagttgc
    42481 gctgtttggt ttgttctcac tggcaacggc gattgcctgg gatttcccct cactggtctt
    42541 tgcgcggctg atgaccggtg tcgggctggg ggcggcgttg ccgaatctta tcgctctgac
    42601 gtctgaagcc gcgggtccac gttttcgtgg gacggcagtg agcctgatgt attgcggtgt
    42661 tcccattggc gcggcgctgg cggcgacact gggtttcgcg ggggcaaact tagcatggca
    42721 aacggtgttt tgggtaggtg gtgtggtgcc gttgattctg gtgccgctat taatgcgctg
    42781 gctgccggag tcagcggttt tcgctggcga aaaacaggct gcgccaccac tgcgtgcctt
    42841 atttgcgcca gaaacggcaa ccgcgacgct gctgctgtgg ttgtgttatt tcttcactct
    42901 gctggtggtt tatatgttga tcaactggct accgctactt ttggtggagc aaggattcca
    42961 gccatcgcag gcggcagggg tgatgtttgc cctgcaaatg ggggcggcaa gcgggacgtt
    43021 aatgttgggc gcattgatgg ataagctgcg tccagtaacc atgtcgctac tgatttatag
    43081 cggcatgtta gcttcgctgc tggcgcttgg aacggtgtcg tcatttaacg gtatgttgct
    43141 ggcgggattt gtcgcggggt tgtttgcgac aggtgggcaa agcgttttgt atgccctggc
    43201 accgttgttt tacagttcgc agatccgcgc aacaggtgtg ggaacagccg tggcggtagg
    43261 gcgtctgggg gctatgagcg gtccgttact ggccgggaaa atgctggcat taggcactgg
    43321 cacggtcggc gtaatggccg cttctgcacc gggtattctt gttgctgggt tggcggtgtt
    43381 tattttgatg agtcggagat caagaatgca gccgtgtgcc gatgcctgac gcgccttatt
    43441 tagcaggggc gaaagggact tgcccccaat acacccacgg cgtttacagg tatactcgct
    43501 aaaaattatt cagcgggttt ggaaacaaag atggcaaaac ttaccttaca agagcagttg
    43561 ctcaaagcag gattagtcac cagcaaaaaa gcggcgaagg tggagagaac ggcgaaaaaa
    43621 tcgcgcgttc aggcgcgtga agctcgggcg gcggtagaag aaaataaaaa ggcacagctt
    43681 gagcgtgata aacagcttag cgctgagaga tcccctcata atttccccaa aacgtaacca
    43741 tgtgtgaata gattttgagt aagcagggtt gcagccacga gtgagtcttc ccttgttatt
    43801 gtgtagccag aatgccgcaa aacttccatg cctaagcgaa ctgttgagag tacgtttcga
    43861 tttctgactg tgttagcctg gaagtgcttg tcccaacctt gtttctgagc atgaacgccc
    43921 gcaagccaac atgttagttg aagcatcagg gcgattagca gcatgatatc aaaacgctct
    43981 gagctgctcg ttcggctatg gcgtaggcct agtccgtagg caggactttt caagtctcgg
    44041 aaggtttctt caatctgcat tcgcttcgaa tagatattaa caagttgttt gggtgttcga
    44101 atttcaacag gtaagttagt tgctagaatc catggctcct ttgccgacgc tgagtagatt
    44161 ttaggtgacg ggtggtgaca atgagtccgt gtcgagcgct gattttttcg gcctttagag
    44221 cgagatttat acaatagaat ttggcatgag attggattgc ttttagtcag cctcttatag
    44281 cctaaagtct ttgagtgact agatgacata tcatgtaagt tgctgatagg tttccagttt
    44341 tccgctccta ggtctgcata ttgtactttt cctcttactc gacttaacca gtaccaaccc
    44401 agcttctcaa cggatttata ccatggcact ttaaagccag catcactgac aatgagcggt
    44461 gtggtgttac tcggtagaat gctcgcaagg tcggctagaa attggtcatg agctttcttt
    44521 gaacattgct ctgaaagcgg gaacgctttc tcataaagag taacagaacg accgtgtagt
    44581 gcgactgaag ctcgcaatac cataagccgt ttttgctcac ggatatcaga ccagtcaaca
    44641 agtacaatgg gcatcgtatt gcccgaacag ataaagctag catgccaacg gtatacagcg
    44701 agtcgctctt tgtggaggtg acgattacct aacaatcggt cgattcgttt gatgttat
//
LOCUS       DBANUU010000024        44595 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000024 DBANUU010000000
VERSION     DBANUU010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.024"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            180..1274
                     /gene="gcvT"
                     /locus_tag="ACIE68_001272"
     CDS             180..1274
                     /gene="gcvT"
                     /locus_tag="ACIE68_001272"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFX5907618.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMGETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1298..1687
                     /gene="gcvH"
                     /locus_tag="ACIE68_001273"
     CDS             1298..1687
                     /gene="gcvH"
                     /locus_tag="ACIE68_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFX5907619.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1805..4678
                     /gene="gcvP"
                     /locus_tag="ACIE68_001274"
     CDS             1805..4678
                     /gene="gcvP"
                     /locus_tag="ACIE68_001274"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFX5907620.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTIGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            4944..5687
                     /gene="ygfF"
                     /locus_tag="ACIE68_001275"
     CDS             4944..5687
                     /gene="ygfF"
                     /locus_tag="ACIE68_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFX5907621.1"
                     /translation="MAIALVTGGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5744..7177)
                     /gene="bglA"
                     /locus_tag="ACIE68_001276"
     CDS             complement(5744..7177)
                     /gene="bglA"
                     /locus_tag="ACIE68_001276"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFX5907622.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            7222..7533
                     /gene="yqfB"
                     /locus_tag="ACIE68_001277"
     CDS             7222..7533
                     /gene="yqfB"
                     /locus_tag="ACIE68_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFX5907623.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            7697..8356
                     /gene="yqfA"
                     /locus_tag="ACIE68_001278"
     CDS             7697..8356
                     /gene="yqfA"
                     /locus_tag="ACIE68_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFX5907624.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8433..9413)
                     /gene="ygfZ"
                     /locus_tag="ACIE68_001279"
     CDS             complement(8433..9413)
                     /gene="ygfZ"
                     /locus_tag="ACIE68_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFX5907625.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9656..9922
                     /gene="sdhE"
                     /locus_tag="ACIE68_001280"
     CDS             9656..9922
                     /gene="sdhE"
                     /locus_tag="ACIE68_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFX5907626.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            9903..10310
                     /gene="ygfX"
                     /locus_tag="ACIE68_001281"
     CDS             9903..10310
                     /gene="ygfX"
                     /locus_tag="ACIE68_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFX5907627.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10350..10871)
                     /gene="fldB"
                     /locus_tag="ACIE68_001282"
     CDS             complement(10350..10871)
                     /gene="fldB"
                     /locus_tag="ACIE68_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFX5907628.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            10983..11879
                     /gene="xerD"
                     /locus_tag="ACIE68_001283"
     CDS             10983..11879
                     /gene="xerD"
                     /locus_tag="ACIE68_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFX5907629.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            11904..12614
                     /gene="dsbC"
                     /locus_tag="ACIE68_001284"
     CDS             11904..12614
                     /gene="dsbC"
                     /locus_tag="ACIE68_001284"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFX5907630.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            12620..14353
                     /gene="recJ"
                     /locus_tag="ACIE68_001285"
     CDS             12620..14353
                     /gene="recJ"
                     /locus_tag="ACIE68_001285"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFX5907631.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMILKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14444..15542
                     /gene="prfB"
                     /locus_tag="ACIE68_001286"
     CDS             join(14444..14518,14520..15542)
                     /gene="prfB"
                     /locus_tag="ACIE68_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFX5907632.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15552..17069
                     /gene="lysS"
                     /locus_tag="ACIE68_001287"
     CDS             15552..17069
                     /gene="lysS"
                     /locus_tag="ACIE68_001287"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFX5907633.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     GLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17112..17660)
                     /gene="idi"
                     /locus_tag="ACIE68_001288"
     CDS             complement(17112..17660)
                     /gene="idi"
                     /locus_tag="ACIE68_001288"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFX5907634.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            17715..17786
                     /locus_tag="ACIE68_001289"
     CDS             17715..17786
                     /locus_tag="ACIE68_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5907635.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKSI"
     gene            complement(17783..17908)
                     /gene="yqfG"
                     /locus_tag="ACIE68_001290"
     CDS             complement(17783..17908)
                     /gene="yqfG"
                     /locus_tag="ACIE68_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFX5907636.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(17910..19358)
                     /gene="uacT"
                     /locus_tag="ACIE68_001291"
     CDS             complement(17910..19358)
                     /gene="uacT"
                     /locus_tag="ACIE68_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFX5907637.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            19794..21713
                     /gene="ygfT"
                     /locus_tag="ACIE68_001292"
     CDS             19794..21713
                     /gene="ygfT"
                     /locus_tag="ACIE68_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFX5907638.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     VAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVTIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSNL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            21713..22201
                     /gene="ygfS"
                     /locus_tag="ACIE68_001293"
     CDS             21713..22201
                     /gene="ygfS"
                     /locus_tag="ACIE68_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFX5907639.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(22237..23604)
                     /gene="ghxQ"
                     /locus_tag="ACIE68_001294"
                     /gene_synonym="ygfQ"
     CDS             complement(22237..23604)
                     /gene="ghxQ"
                     /locus_tag="ACIE68_001294"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFX5907640.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(23640..24956)
                     /gene="guaD"
                     /locus_tag="ACIE68_001295"
     CDS             complement(23640..24956)
                     /gene="guaD"
                     /locus_tag="ACIE68_001295"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFX5907641.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(24974..26374)
                     /gene="xanQ"
                     /locus_tag="ACIE68_001296"
     CDS             complement(24974..26374)
                     /gene="xanQ"
                     /locus_tag="ACIE68_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFX5907642.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(26539..29409)
                     /gene="xdhD"
                     /locus_tag="ACIE68_001297"
     CDS             complement(26539..29409)
                     /gene="xdhD"
                     /locus_tag="ACIE68_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFX5907643.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKRDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(29406..30185)
                     /gene="ygfM"
                     /locus_tag="ACIE68_001298"
     CDS             complement(29406..30185)
                     /gene="ygfM"
                     /locus_tag="ACIE68_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFX5907644.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLASPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(30236..31564)
                     /gene="ssnA"
                     /locus_tag="ACIE68_001299"
     CDS             complement(30236..31564)
                     /gene="ssnA"
                     /locus_tag="ACIE68_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFX5907645.1"
                     /translation="MLILKNVTAVQLHPAKVQEAVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFAAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDTLA"
     gene            complement(31567..34665)
                     /gene="ygfK"
                     /locus_tag="ACIE68_001300"
     CDS             complement(31567..34665)
                     /gene="ygfK"
                     /locus_tag="ACIE68_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFX5907646.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVKKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(34987..35565)
                     /gene="mocA"
                     /locus_tag="ACIE68_001301"
     CDS             complement(34987..35565)
                     /gene="mocA"
                     /locus_tag="ACIE68_001301"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFX5907647.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITAQKRYTKI"
     gene            35668..36438
                     /gene="yqeC"
                     /locus_tag="ACIE68_001302"
     CDS             35668..36438
                     /gene="yqeC"
                     /locus_tag="ACIE68_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFX5907648.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            36486..38111
                     /gene="yqeB"
                     /locus_tag="ACIE68_001303"
     CDS             36486..38111
                     /gene="yqeB"
                     /locus_tag="ACIE68_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFX5907649.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFI
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(38152..39084)
                     /gene="arcC"
                     /locus_tag="ACIE68_001304"
     CDS             complement(38152..39084)
                     /gene="arcC"
                     /locus_tag="ACIE68_001304"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFX5907650.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(39132..40517)
                     /gene="hyuA"
                     /locus_tag="ACIE68_001305"
     CDS             complement(39132..40517)
                     /gene="hyuA"
                     /locus_tag="ACIE68_001305"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFX5907651.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNIREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(40570..41781)
                     /gene="ygeY"
                     /locus_tag="ACIE68_001306"
     CDS             complement(40570..41781)
                     /gene="ygeY"
                     /locus_tag="ACIE68_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFX5907652.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(41839..43035)
                     /gene="dpaL"
                     /locus_tag="ACIE68_001307"
     CDS             complement(41839..43035)
                     /gene="dpaL"
                     /locus_tag="ACIE68_001307"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFX5907653.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(43093..44280)
                     /gene="ygeW"
                     /locus_tag="ACIE68_001308"
     CDS             complement(43093..44280)
                     /gene="ygeW"
                     /locus_tag="ACIE68_001308"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFX5907654.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT        10818 a        11205 c        11793 g        10779 t
ORIGIN      
        1 ttaaccataa gctaatgtga tgatcaattt taccttatgg ttaacagtct gtttcggtgg
       61 taagttcagg caaaagagaa cgattgcgtt ggggaccggg agtggctccg atgctgggtt
      121 tcgtggtgat aatttcacta tgaaaaagtt gtcagccccg cttattcaat gaggacaaga
      181 tggcacaaca gactcctttg tacgaacaac atacgctttg cggcgctcgc atggtggatt
      241 tccacggctg gatgatgccg ctgcattacg gttcgcaaat cgacgaacat catgcggtac
      301 gtaccgatgc cggaatgttt gatgtgtcac atatgaccat cgtcgatctt cgcggcagcc
      361 gcacccggga gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg
      421 gcaaagccct ttactcgggg atgttgaatg cctctggcgg tgtgatagat gatctcatcg
      481 tctactactt tactgaagat ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag
      541 acctctcctg gattacccaa cacgctgaac ctttcggcat cgaaattacc gttcgtgatg
      601 acctttccat gattgccgta caagggccga atgcgcaggc aaaagctgcc acactgttta
      661 atgatgctca gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg caggcgggcg
      721 atctgtttat tgccaccact ggttataccg gtgaagcggg ctatgaaatt gcgctgccca
      781 atgaaaaagc ggcggatttc tggcgcgcgc tggtggaagc gggcgttaag ccatgcggcc
      841 tgggcgcgcg tgacacgctg cgtctggaag cgggcatgaa tctttatggt caggagatgg
      901 gcgaaactat ttctccttta gccgccaaca tgggctggac tatcgcctgg gaaccggcag
      961 atcgtgactt catcggtcgt gaagccctgg aagtgcagcg tgagcatggc acagaaaaac
     1021 tggttggtct ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg gtacgtttta
     1081 ccgatgcgca gggcaaccag catgaaggca ttatcaccag cgggactttc tccccgacgc
     1141 tgggttacag cattgcgctg gcacgcgtgc cggaaggtat tggcgaaacg gcgattgtgc
     1201 aaattcgcaa ccgtgaaatg ccggttaaag tgacgaaacc tgtttttgtg cgtaacggta
     1261 aagccgtcgc gtgatttact tttttggaga ttgattgatg agcaacgtac cagcagaact
     1321 gaaatacagc aaagaacacg aatggctgcg taaagaagcc gacggcactt acaccgttgg
     1381 tataaccgaa catgcccagg agctgttagg cgatatggtg tttgttgacc tgccggaagt
     1441 gggcgcaacg gttagcgcgg gcgatgactg cgcggttgcc gaatcagtaa aagcggcgtc
     1501 agacatttat gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac tgagcgattc
     1561 cccggaactg gtgaacagcg aaccgtatgc aggtggctgg atctttaaaa tcaaagccag
     1621 cgatgaaagc gaactggaat cactgctgga tgcgaccgca tacgaagcat tgttagaaga
     1681 cgagtaacgg ctttattcct cttctgcggg agaggatcag ggtgaggata tttacgcctc
     1741 accctcactc tcttcgcaag gagagaggtt cacaattcac tgcacgtttc aggaaccatc
     1801 gctcatgaca cagacgttaa gccagcttga aaacagcggc gcttttattg aacgccatat
     1861 cggaccggac gccgcgcaac agcaagaaat gctgaatgcc gttggtgcac aatcgttaaa
     1921 cgcgctgacc ggccagattg tgccgaaaga tattcagctt gcgaccccgc cgcaggttgg
     1981 cgcaccggcg accgaatacg ccgcactggc agaactcaag gctattgcca gtcgcaataa
     2041 acgcttcacg tcttacatcg gcatgggtta caccgccgtg cagctaccgc cggttatcct
     2101 gcgtaacatg ctggaaaatc cgggctggta taccgcgtac actccgtatc aacctgaagt
     2161 ctcccagggc cgccttgaag cactgctcaa cttccagcag gtaacgctgg atttgactgg
     2221 actggatatg gcctctgctt ctcttctgga cgaggccacc gctgccgccg aagcaatggc
     2281 gatggcgaaa cgcgtcagca aactgaaaaa tgccaaccgc ttcttcgtgg cttccgatgt
     2341 gcatccgcaa acgctggatg tggtccgtac tcgtgccgaa acctttggtt ttgaagtgat
     2401 tgtcgatgac gcgcaaaaag tgctcgacca tcaggacgtc ttcggcgtgc tgttacagca
     2461 ggtaggcact atcggtgaaa ttcacgacta cactgcgctt attagcgaac tgaaatcacg
     2521 caaaattgtg gtcagcgttg ccgccgatat tatggcgctg gtgctgttaa ctgcgccggg
     2581 taaacagggc gcggatattg tttttggttc ggcgcaacgc ttcggcgtgc cgatgggcta
     2641 cggtggccca cacgcggcat tctttgcggc gaaagatgaa tacaaacgct caatgccggg
     2701 ccgtattatc ggtgtatcga aagatgcagc tggcaatacc gcgctgcgca tggcgatgca
     2761 gactcgcgag caacatatcc gccgtgagaa agcgaactcc aacatttgta cttcccaggt
     2821 actgctggca aacatcgcca gcctgtatgc cgtttatcac ggcccggttg gcctgaaacg
     2881 tatcgctaac cgcattcacc gtttgaccga tatcctggcg gcaggcctgc aacaaaaagg
     2941 tctgaagctg cgccatgcgc actatttcga cactctgtgt gtggaagtgg ccgacaaggc
     3001 gggcgtactg gcgcgtgccg aagcggctga aatcaacctg cgtagcgata tcctgaacgc
     3061 ggttgggatc acccttgatg aaaccaccac gcgtgaaaac gtaatgcagc ttttcagcgt
     3121 gctgctgggc gataaccacg ggctggagat cgacacgctg gacaaagacg tggctcacga
     3181 cagccgctct atccagcctg cgatgctgcg cgacgacgaa atcctcaccc atccggtgtt
     3241 taatcgctac cacagcgaaa ccgaaatgat gcgctatatg cactcgctgg agcgtaaaga
     3301 tctggcgctg aatcaggcga tgatcccgct gggttcctgc accatgaaac tgaacgccgc
     3361 cgccgagatg atcccaatca cctggccgga atttgccgaa ctgcacccgt tttgtccgcc
     3421 ggaacaggct gaaggttatc agcagatgat tgcgcagctg gctgactggc tggtgaaact
     3481 gaccggttac gacgccgttt gtatgcagcc gaactccggc gcacagggcg aatacgcggg
     3541 cctgctggcg attcgtcatt atcatgaaag ccgcaacgaa gggcatcgcg atatctgcct
     3601 gatcccggct tctgcgcacg gaactaaccc cgcttctgca catatggcag gaatgcaggt
     3661 ggtggttgtg gcgtgtgata aaaacggcaa catcgatctg actgatctgc gcgcgaaagc
     3721 ggaacaggcg ggcgataacc tctcctgtat catggtgact tatccttcta cccacggcgt
     3781 gtatgaagaa acgatccgtg aagtgtgtga agtcgtgcat cagttcggcg gtcaggttta
     3841 ccttgatggc gcgaacatga acgcccaggt tggcatcacc tcgccgggct ttattggcgc
     3901 ggacgtttcg cacctcaacc tgcataaaac tttctgcatt ccgcacggcg gtggtggtcc
     3961 gggtatggga ccgatcggtg tgaaagcgca tctggcaccg tttgtaccgg gtcatagcgt
     4021 ggtgcaaatc gaaggcatgt taacccgtca gggcgcagtt tctgcggcac cgttcggtag
     4081 cgcctctatc ctgccaatca gctggatgta catccgcatg atgggcgcag aagggctgaa
     4141 aaaagcaagc caggtggcaa tcctcaacgc caactatatt gccagccgcc tgcaggatgc
     4201 cttcccggtg ctgtataccg gtcgcgacgg tcgcgtggcg cacgaatgta ttctcgatat
     4261 tcgcccgctg aaagaagaaa ccggcatcag cgagctggat attgccaagc gcctgatcga
     4321 ctacggtttc cacgcgccga cgatgtcgtt cccggtggcg ggtacgctga tggttgaacc
     4381 gactgaatct gaaagcaaag tggaactgga tcgctttatc gacgcgatgc tggctatccg
     4441 cgcagaaatc gaccaggtta aagccggtgt ctggccgctg gaagataacc cgctggtgaa
     4501 cgcgccgcac attcagaacg aactggtcgc cgagtgggcg catccgtaca gccgtgaagt
     4561 tgcagtattc ccggcaggtg tggcagacaa atactggccg acggtgaaac gtctggatga
     4621 tgtttacggc gaccgtaacc tgttctgctc ctgcgtaccg attagcgaat accagtaatt
     4681 cactgattcg actatcttct aaaggcgctt cggcgccttt ttagtcagat gacaaagtac
     4741 aaaagcgctc agacagtccc ctcacccctt tggggagagg gttagggtga ggggaacagg
     4801 ccggcactgg cgcgaatatt taccctcacc ccggccctct ccctgaaagg gcgaggggaa
     4861 aagcgtgcca acattgaaga ttgagccagt ttgttagcaa tctcaaagat acgtcaacga
     4921 attaattttt ctcggaaaaa caaatggcta tagcacttgt gaccggtggc agtcgcggca
     4981 tcgggcgggc aactgcatta ctgttggcga aagaagagta tacggtggcg gttaattatc
     5041 aacaaaacct ccatgcggcg caggaagtgg tgaacttaat cacgcaagcc ggtggcaagg
     5101 cattcgtgct ccaggcggat atcagcgacg aaaatcaggt catcgcaatg tttacagcaa
     5161 tcgatcagca cgatgaaccg ctagcagcgc tggtcaataa cgccgggatc ttgtttaccc
     5221 agtgcaccgt tgaaaacctt accgcagagc gaatcaaccg agtactttcc accaacgtga
     5281 cgggatattt tctctgctgc cgcgaggcgg taaaacgcat ggcgcttaaa aatggtggca
     5341 gtggcggcgc tatcgtcaat gtctcttcgg tggcctcacg gttgggttcg ccaggggaat
     5401 atgttgatta cgcggcatcg aaaggggcga ttgatacgtt aaccaccgga ctatcgctgg
     5461 aagtcgccgc gcaggggatc cgcgttaact gcgtgcggcc agggtttatt tataccgaaa
     5521 tgcacgccag cggcggcgag cctggacgcg tcgatcgcgt taagtcgaac atccccatgc
     5581 agcgtggtgg acaggcagaa gaggtcgcgc aggccattgt ctggctacta agtgataaag
     5641 cctcttacgt cacgggaagt tttatcgatt tggcgggcgg gaaataaaac agggaagttg
     5701 tctgaccgga tgcaacaagt attgcatccg gtacttcatc gacttaaagc ttctcgccgt
     5761 tgctggcaat cacctctttg taccagttaa agctcttctt acgtgaacgc gacatatcgc
     5821 cagtaccgtc gtcatgttta ttcacataga taaagccgta gcgtttgctg tactgcccgg
     5881 tagtgaatga cacgcagtcg atgcagcccc acggtgtgta gcccatcaga tccacaccat
     5941 cgtaagtcac tgcttttttc atctcttcaa tatgggcgcg caggtagtca atgcggtagt
     6001 cgtcgttgat gctgccatct tcttccactt tgtcgtaagc gccaaaaccg ttttcgacaa
     6061 taaacagcgg cctctgataa cgctcataca gttcgcaaag ggcatagcgc agacctactg
     6121 gatcaatctg ccagccccag tcagacgctt taacatacgg gttcggtacg ctgccttcaa
     6181 aaccagagat cgcatcgcct gtgccgcctt cggccttcac tgcgttggtc atgtaatagc
     6241 tgaaaccaag ataatcgcag gtgccttcac gcagtacatc gagatcgccg tcttccattt
     6301 tgatgttaaa tccgcgacgc tcccactcgt tcaacacata ggacgggtaa tagccgcgta
     6361 gctgcacatc ggtaaagacg tagcgttcgc gcatcgactc ctgagcgaac atcacatcgt
     6421 ccgggttaca gcagtaggga tagagcggca ccatcgccag catacagccg actttcatct
     6481 ccgggttaat gcgacgcgca gctttcaccg ccagggcgct ggcgacaaac tggtgatgca
     6541 gcacctgata catcgtctct tccgggttct catgctcggt ataaaccacg ccggagcagc
     6601 agtaaccgaa cagcggtgca cgccagttac gctggttgtt aatttcgttg aaggtcatcc
     6661 agtatttgac tttatgctta tagcgttcaa acaccacttc cgcgaaacgg acaaagaaat
     6721 caaccacttt acggttggtc cagctaccgt attgctgcac cagatgcagc ggcatttcaa
     6781 agtgggagag ggtgatcacc ggttcgatgt tgtatttcag cagttcatcg aacatatcat
     6841 cgtagaactt cagcccttct tcgtttggct gagcttcatc accttttgga aaaatacgcg
     6901 tccaggcgat ggaagtccga aaacatttga agcccatttc ggcaaatagc ttgatgtctt
     6961 ccttgtagtg accataaaaa tcaacggctt catggtttgg atagtatttg cctggcaaga
     7021 cttctttggt gatttcgcgc ggcacgccgt gtgcgccacc ggtcagaacg tcacaaatgc
     7081 tcggcccttt gccgcctttg ttccagccgc cttcgacctg atgagcggca actgcgccgc
     7141 cccataagaa atctttcggt aaggtgagtt ttttcactat cataggctcc agatgagtca
     7201 attcttacag gaaatagtac catgcagcca aacgacatca ctttttttca acgtttccag
     7261 gatgacattc tggctgggcg taaaaccatc accatccgcg acgagtctga atcgcacttc
     7321 aaaacgggtg atgtgcttcg tgtcgggcgt tttgaagatg acggttattt ttgcacgatt
     7381 gaagtcaccg caacctcaac cgtaacgctg gatacgctga cggaaaaaca tgcagagcag
     7441 gaaaatatga ccctgactga actgataaag gtcattgccg acatctatcc cggtcagaca
     7501 cagttttatg tgattgaatt taaatgtctt taaattgaac tcacgcctgt tagttgaaat
     7561 ttcgcattta atttcatgat ttttaggaga ttagtctcaa atcagaattt attttagcta
     7621 acaggtgttc actggaacta ttctcagtta cgctggagag gtatacatgt gcgtgtacgt
     7681 ttctccggag taagttatgg ttcagaagcc cctcattaag cagggatatt cgctggcaga
     7741 ggaaattgcc aacagcgtca gtcacggcat tgggttggtg tttggtatcg ttgggctggt
     7801 gttgctactg gttcaggcgg tggatctcaa tgccagcgcc acagcgataa ccagttacag
     7861 cctctatggc ggcagtatga tcctgctgtt cctcgcttcg acgctctatc acgccattcc
     7921 tcatcaacgg gcaaaaatgt ggctgaagaa atttgaccat tgcgctattt acctgttgat
     7981 tgccggaacc tacacgccgt ttttgctggt ggggctggat tctccgttag cgcgcgggtt
     8041 gatgattgtt atctggagcc tggcattgct gggtattctg tttaaactga ccatcgcgca
     8101 ccgattcaaa attttatctc tggtgaccta tctggcgatg ggctggctgt cgctggtggt
     8161 aatttatgaa atggcagtta agctcgcggc gggcagcgtt accttactgg cggtaggcgg
     8221 cgtggtttat tcgctcgggg tgattttcta cgtctgcaaa cgcattccat acaaccatgc
     8281 catctggcac ggcttcgtgc tcggcggtag tgtgtgccac tttctggcga tctatttgta
     8341 tattgggcag gcgtaatggg tatgcctttg taggcctgat aagacgcgcc agcgtcgcat
     8401 caggcgataa atgcctgatg cgataaagag atttactctt cgagcgaata cggcagcggc
     8461 tcgatatgca gtgtattcgc atcgtcgcgt acgcggaaga tgctatccgg ttccatatcg
     8521 ttattcatga cgacctgtac cacgacctga ccatcttcca gttttaccgc agccagcacc
     8581 gtaccggtac gacgccagtt ctcgcccatt ttcagctcta agtcttcacc agcttccggc
     8641 agtcggctgg cgctacctgc cagcaaccag agcgcacgtt tattggcacc acggaatttt
     8701 gctcgcgcca ccatctcttg tccggtatag cagccttttt taaagctgat accgcccagc
     8761 gcctggaggt tggtcgcctg tgggataaac tgcccgctgt tggcggcatc aatcaccggg
     8821 aaacccgctt caatgtttaa tgccagccac tgttggctat tgttcagttc cgcttcaccg
     8881 cgcagtttat cggtcagcat attagcagta gcttcatcgg ttacgatcag gaaacgttct
     8941 gccgggtgtt caaaccatag caaagtggtc gcgccttctt taactacctg tttttctttc
     9001 gaaggcagtt cactaaagag atttgccagc gcggcgcgcg cctgaaaacc ggcaacacca
     9061 agcagcacac gctcgtcgtc tggcgcgatg gtcactttag agaataccgc atattttttc
     9121 agttcagtca gctgcggttc acgcacgctg cgccgttcaa tccatgcaaa gccatcgccg
     9181 tcgcggaaca gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc
     9241 aggtgctgat cttctgccat ctggctgaca tctgctgtca cctgaccctg catatatttt
     9301 tcgctgtccg cgccagtaat ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc
     9361 ggtaaacggg cagaagccgt aggctgacgg ggaggaaaag gtgtaaaagc cataatagag
     9421 tcctgagttg cttaacggcg tattaatgac taatggtaaa agagctatga ggcaatgcaa
     9481 gtgctttcac tacgcgatct gtgccttctg cgaggcgact tccaaaaaaa tgaagccatt
     9541 gaattgttgt gaattaattt cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa
     9601 ggcgaaaaac acgttacaat agcggggtac tggattttcg tgagacagga agaagatgga
     9661 cattaacaac aaagcccgca ttcattgggc atgccgccgt ggtatgcgcg aactcgatat
     9721 ttcaatcatg ccgtttttcg aacatgagta cgacagctta agcgatgacg aaaaacgcat
     9781 ctttattcgt ctgctggaat gtgacgatcc ggacctgttt aactggctga tgaatcacgg
     9841 taaaccggcc gatgcagaac tggaaatgat ggtccgactc atccagacac ggaaccggga
     9901 acgtggtcct gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc
     9961 tgattcatgg gctggttgcc gctgttattt tactcatgcc ctggccactc agttacaccc
    10021 cgttatggat ggtgttactt tcgctggtgg tgtttgattg cgttcgcagc cagcggcgta
    10081 ttaatgctcg ccagggggaa attcgcttgt tgatggacgg gcgtttgcgt tggcaagggc
    10141 aggagtggag catcgtcaaa gcaccgtgga tgattaagag cggcatgatg ctgcgtttac
    10201 gttctgatgg cggtaaacgg caacatttat ggctggcagc cgacagcatg gacgaagccg
    10261 aatggcggga tttacggcgg attttgttgc aacaagagac gcaaagataa agatgacggc
    10321 aaagcatatg cgctaagccg tcagtgagat caggcgtaat gctctgccat ttcgttgagg
    10381 atttgctcgc accagctctg aatacgctcg tcgctaaggt catactggtt agtttcatcc
    10441 agcgccagac ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca
    10501 tatccttccg ttggccagta gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc
    10561 agcataccga gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc aagcccatac
    10621 agcgcaacaa ttttaccttc aaggttcagg tcgtcgagct gatcccagac ggcttcccag
    10681 tcttcttgga tttcaccaaa atcccaggtc gggataccca gaatgagcac atcgtactgc
    10741 tccattaatt tcggggagtc gtccttgagg ttatgtaagg tcaccagttc tgggccgata
    10801 atatcgcgga ttttttctgc cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga
    10861 cccatattca taacgtaagc ttctcgattc tgctgtggct tttgcggcta gtgtatcaga
    10921 atcgctttag tcacggcaca ataaggcata atggatcaaa tgagtgacca taaggggcgc
    10981 aagtgaaaca ggatctggca cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa
    11041 atctggctga aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt
    11101 tgcatcaccg cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag gcattactgg
    11161 cagaacggct ggagggcggg tataaagcca ccagctcagc gcgtttgctg agtgcagtgc
    11221 gccgattgtt ccagtatctt tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc
    11281 tcgcttcacc gaaattgccc cagcgtttgc caaaagattt aagcgaagcg caggttgaac
    11341 gtttattaca ggcaccatta attgatcagc cactggagct acgcgataaa gccatgcttg
    11401 aagtgttgta tgctaccggg ctgcgtgtct ctgaactggt cggactgaca atgagtgata
    11461 tcagcctgcg tcagggcgta gtgcgggtca ttggtaaagg caacaaagag cgcctggtac
    11521 cgttaggtga agaggcggtt tactggctgg aaacctatct ggaacatggg cgtccgtggc
    11581 tgctgaatgg tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc
    11641 gacagacctt ctggcaccgc attaaacatt atgctgtgct ggcgggtatc gacagcgaaa
    11701 agctgtcacc gcatgtgttg cgtcacgctt ttgccaccca tttattaaat catggtgcgg
    11761 atttacgcgt ggtgcagatg ctactgggcc acagcgacct ctccaccacg caaatttata
    11821 cgcatgtcgc taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat
    11881 gtctgaaaag aacgggaaga tttatgaaga aaggttttat gttgtttact ttgttagcgg
    11941 cgttttcagg ttttgctcag gctgatgacg cggcaattca acaaacgtta gccaaaatgg
    12001 gcatcaaaag cagcgatatt cagcccgcgc ccgtagctgg catgaagaca gttctgacta
    12061 acagcggcgt gttgtacatc accgatgatg gtaaacatat cattcagggg ccaatgtatg
    12121 acgttagcgg cacggctccg gtcaatgtca ccaataagat gctgttaaag cagttgaatg
    12181 cgcttgaaaa agagatgatc gtttataaag cgccgcagga aaaacacgtc atcaccgtgt
    12241 ttactgatat tacctgtggt tactgccaca aactgcatga gcaaatggca gactataacg
    12301 cgctggggat caccgtgcgt tatcttgctt tcccgcgcca ggggctggac agcgatgcag
    12361 agaaagaaat gaaagctatc tggtgtgcga aagataaaaa caaagcgttt gatgatgtga
    12421 tggcaggtaa aagcgtcgca ccagccagct gcgacgtgga tattgccgac cattacgcac
    12481 ttggcgtcca gcttggcgtt agcggtactc cggcagttgt gctgagcaat ggcacacttg
    12541 ttccgggtta ccagccgccg aaagagatga aagaattcct cgacgaacac caaaaaatga
    12601 ccagcggtaa ataattcgcg tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga
    12661 aacggcagac ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg ccagccgggg
    12721 tgtgcgcagt gcgcaagaac tggaacgcag tgttaaaggt atgttgccct ggcagcaact
    12781 gagcggcgtc gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag gaacgcggat
    12841 tattgtggtc ggcgattttg acgccgacgg cgcgaccagc acggctctaa gcgtgctggc
    12901 gatgcgctcg cttggttgca gcaatatcga ctatctggta ccaaaccgtt tcgaagacgg
    12961 ttacggctta agcccggaag tagtcgatca ggcccatgcc cgtggcgcgc agttaattgt
    13021 cacggtggat aacggtattt cctcccatgc gggcgttgaa cacgctcgct cgttgggcat
    13081 tccggttatt gttaccgatc accatttgcc gggcgaaaca ttacccgcag cggaagcgat
    13141 cattaaccct aacttgcgcg actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt
    13201 ggcgttttat ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct ggtttgatga
    13261 gcgtggcatc gcaattccta acctggcaga actgctggat ctggtagcgc tggggacagt
    13321 agcggacgtc gtgccgctgg acgctaataa tcgcattctg acctggcagg ggatgagtcg
    13381 catccgtgcc ggaaagtgcc gtccggggat taaagcgctg ctggaagtgg caaatcgcga
    13441 tgcgcaaaaa ctcgccgcca gcgatttagg ttttgcgctg gggccacgtc tcaatgctgc
    13501 cggacgactg gacgatatgt ccgtcggtgt ggcgctcttg ctgtgcgaca acatcggcga
    13561 agcgcgcgtg ctggcaaatg aactcgatgc gctaaaccag acgcgaaaag agatcgaaca
    13621 aggaatgcaa gttgaagccc tgaccctgtg cgagaaactg gagcgaagtc gcgacacgct
    13681 acccggcggg ctggcaatgt atcaccccga atggcatcag ggcgttgtcg gtattctggc
    13741 ttcgcgcatc aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag caggtgatgg
    13801 tacgctgaaa ggttcaggtc gctccattca ggggctgcat atgcgtgatg cactggagcg
    13861 attagacaca ctctaccctg gcatgatact gaagtttggc ggtcatgcga tggcggcggg
    13921 tttgtcgctg gaagaggata aattcgaact ctttcaacaa cggtttggcg aactggttac
    13981 agagtggctg gacccttcgc tattgcaagg cgaagtggtg tcagacggtc cgttaagccc
    14041 ggccgaaatg accatggaag tggcgcagtt gctgcgcgat gctggcccgt gggggcagat
    14101 gttcccggag ccgctgtttg acggtcattt ccgtctgctg caacagcggc tggtgggcga
    14161 acgtcatttg aaggtgatgg tcgaaccggt cggcggcggt ccactgctgg atggtattgc
    14221 ttttaatgtc gataccgccc tctggccgga taacggcgtg cgtgaagtgc aactggctta
    14281 taagctcgat atcaacgagt ttcgcggcaa ccgcagcctg caaattatca tcgacaatat
    14341 ctggccaatt tagcgtcatc ttctctataa aaaagagcgt ggattgggta caatcccgct
    14401 cttatcaccg cattttgact agctcaataa aagaaatcag accatgtttg aaattaatcc
    14461 ggtaaataat cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatctttg
    14521 actacgacgc caagaaagag cgtctggaag aagtaaacgc cgagctggaa cagccggatg
    14581 tctggaacga acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg
    14641 ttgtcgacac cctcgaccaa atgaaacagg ggctggaaga tgtttctggt ctgctggaac
    14701 tggctgtaga agctgacgac gaagaaacct ttaacgaagc cgttgctgaa ctcgacgccc
    14761 tggaagaaaa actggcgcag cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg
    14821 ccgactgcta cctcgatatt caggcggggt ctggcggtac ggaagcacag gactgggcga
    14881 gcatgcttga gcgtatgtat ctgcgctggg ctgaatcgcg tggtttcaaa actgaaatca
    14941 tcgaagagtc ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa atctccggcg
    15001 attacgctta cggctggctg cgtacagaaa ctggcgttca ccgcctggtg cgtaagagcc
    15061 cgtttgactc cggcggtcgt cgccacacat cgttcagctc tgcgtttgtt tacccggaag
    15121 ttgatgatga tattgatatc gaaatcaacc cggcggattt gcgcattgac gtttatcgcg
    15181 cgtccggcgc gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt attacccaca
    15241 tcccaaccgg gatcgtgacc caatgccaga acgaccgttc ccagcacaag aacaaagacc
    15301 aggccatgaa gcagatgaaa gcgaagcttt atgaactgga gatgcagaag aaaaatgccg
    15361 agaaacaggc gatggaagat aacaaatccg acatcggctg gggcagccag attcgttctt
    15421 atgtccttga tgactcccgc attaaagatc tgcgtaccgg ggtagaaacc cgcaacacgc
    15481 aggccgtgct ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa gcagggttat
    15541 gaggaaccaa catgtctgaa caacacgcac agggcgctga cgcggtagtc gatcttaaca
    15601 atgaactgaa aacgcgtcgt gagaagctgg cgaacctgcg cgagcagggg attgccttcc
    15661 cgaacgattt ccgtcgcgat catacctctg accaattgca cgcagaattc gacggtaaag
    15721 agaacgaaga actggaagcg ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc
    15781 gccgtattat gggtaaagcg tctttcgtta ccctgcagga tgtgggtggc cgcattcagc
    15841 tgtacgttgc ccgtgacggt ctgccggaag gcgtttacaa cgagcagttc aaaaaatggg
    15901 acctcggtga catcctcggc gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt
    15961 ctatccactg taccgagctg cgtctgctga ccaaagcact gcgtccgctg ccggataaat
    16021 tccacggctt gcaggatcag gaagcgcgct atcgtcagcg ttatctggat ctcatctcta
    16081 acgatgaatc ccgtaacacc tttaaagtgc gctcgcagat cctctctggt attcgccagt
    16141 tcatggtgaa ccgcggcttt atggaagttg aaacgccgat gatgcaggtg atccctggcg
    16201 gtgccgctgc gcgtccgttt atcacccacc ataacgcgct ggatctcgac atgtacctgc
    16261 gtatcgcgcc ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg
    16321 aaatcaaccg taacttccgt aacgaaggta tctccgtacg tcataaccca gagttcacca
    16381 tgatggaact ctacatggct tacgcagatt acaaagatct gatcgagctg accgaatcgc
    16441 tgttccgtac tctggcacag gatattctcg gtaagacgga agtgacctat ggcgacgtga
    16501 cgctggactt cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc
    16561 gcccggaaac cgacatggcg gatctggata acttcgactc tgcgaaagcg attgctgaat
    16621 ctatcggtat ccacgttgag aagagctggg gtctgggccg tatcgttacc gagatcttcg
    16681 aagaagtggc agaagcacat ctgattcagc cgaccttcat tactgaatat ccggcagaag
    16741 tttctccgct ggcgcgtcgt aacgacatta acccggaaat cacagaccgc tttgagttct
    16801 tcatcggtgg tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg gaagatcagg
    16861 cgcaacgctt cctggatcag gttgccgcga aagacgcagg tgacgacgaa gcgatgttct
    16921 acgatgaaga ttacgttacc gcactggaac atggcttacc gccgacagca ggtctgggga
    16981 ttggcatcga ccgtatggta atgctgttca ccaacagcca taccatccgc gacgttattc
    17041 tgttcccggc gatgcgtccg gtgaaataag cattacgtta tgctcacaac cccggcaaat
    17101 gtcggggttt tttatttaag ctgggtaaat gcagataatc gtattctggc ttcgcgattt
    17161 gtcgcctgca tcaccatcca cggactgaac gcccacggcg tggcatcaat accgtgtaat
    17221 acatcagcta aatcacacca ttgataatcc atcacttcat catcgttgat ctgtaacgca
    17281 ctggtggtgc gtgcggcaaa taccggacac acttcatttt ccacaatgcc actcggatcg
    17341 gtggcgcggt agcgaaagtc aggatagata gattcaggag gcgtaatttc cacgccaagc
    17401 tcataacggc aacggcggat caccgcgtct tcgctgcttt ctcccagttg tgggtgccca
    17461 caaaccgagt tagtccacac gccaggccac gcttttttgc tcagtgcgcg gcgggtaact
    17521 aataattgcc ccttggcatt aaacagccaa ctggagaacg cgagatgtaa gagggtgtct
    17581 gccgtgtgtg cggcatactt ttccagcgta ccagtgggaa ctccctgtgc attcaataaa
    17641 atgacgtgtt ccgtttgcat aatttctcac atgtaattct gatcactcat cattatagat
    17701 aattgaagag tgaaatgatt aaccaagtga gcgtttatcg acaaccgccc gttttgagcg
    17761 gatgccgaca ggtaaaatcc atttaaaaaa gtagcatcat atatttgaag cgactttcaa
    17821 tggccattgc aacacagtca gaaatgacag gaatagagag agtaaaaagc aacagcagac
    17881 tgaatatagc gcgcattaaa aaattcatat tattctccat gctcattttt cttcagcttg
    17941 cgcatcagaa gccacatacg tactgtcctg acttttaaat ctttatcaga gacggattcc
    18001 ttcaccaggt cagcatgatg ctggtagcca ttaaagaaga cattcagcac aacggcgcta
    18061 agggttgcga gcataatgcc gctatgcagc aacggttgca gtacggccgg taacttagaa
    18121 aagaaatcgt gagagagcgt cggagtcatg ccaacgccga gactgatcgc cacaatatag
    18181 aggttgtaac ggttggtggt gtagttacag cgcgacagaa ttcgaatccc tgtcgccagt
    18241 accatgccga acatcaccag cccagcaccg cccagcacaa attgcggaat ggaggcgacc
    18301 agcaccgcca tttttggcac catgccaaac aggattaata taattcccga agaaatacac
    18361 acccagcggc tatgaacgcg cgtcacgcta accaggccaa cgttttgtga aaatgacgtg
    18421 tgggggaagc tgttaaacgt tccgcctatc attgtcccta cgccatcgac acgcagcccg
    18481 cgaataatat cgtgtgaaga gagtttacga ccgactattt cacccagtgc caggaacatc
    18541 cccatcgact cgataaacac gatgattaac acggcagtca tggtcagaat ggaaacggga
    18601 tcgaaaatcg gcataccaaa tgacatcggc gtgacaatcg caaaccatga agcatcatgt
    18661 agcccggata aattgacttc attcatcatc cacgaaagta aaaagccaaa tacaatcccc
    18721 agtaatacgg cgacgttgga cataaatcct ttcgcatagc gagtaatgag caagataaaa
    18781 attaagacag caaaggagat acctaaataa acgggattac catattgcgg attcccttta
    18841 cctccggcgg cccagtcaat acccacctga atgatgctaa gcccaataga agtaataacc
    18901 acaccggtaa ccagtggcgg gaataaaggc atcaagcgac cgataagtgg cgctaataat
    18961 gtggtgataa aacccgcggc gatagtggca ccaaatatcc ccagcaggcc gatatccggg
    19021 ttcatcccaa tggctatcat tggtgttaca gcagcaaagg tcaccgacat aatcaccggc
    19081 aggcggatcc ccataaagcg gccgataccg atacattgca ataatgtgac gatcccgcag
    19141 caaaagagat ccgagctaat gagcatcgca atagcatctt ttgagaggcc cagtcggtca
    19201 ccaatcataa gaggaacagc gactgcacct gcgtacatga caaggacgtg ttgcagaccg
    19261 agtatgatca gctttcctgg tgataatatg cggtcaacct catcagttgg gcggtcttgc
    19321 ccagaagatg agggaagttg ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc
    19381 cagagtaaaa agcaaccgtt tacgttaatg aaactgtcta aacacatgct cgtggagtgc
    19441 cagtttttgc gaggaagggg agttagagac aggaattgcg gaggcaatca cataatcgtg
    19501 cttaaaccac gaaaatgaca agagaacctg acaatggaat tatctgtaat tttctcatat
    19561 tgatatttca ttaatcattc tggcatgtcg cactctcgca tttaatcgtt tttatctgga
    19621 tagcgctctt ttggtcggca atgttgaaat aatgaaagcg gcatagattg aggggcaata
    19681 atttgaactc agtcgcaaaa ttataattaa aacggtcatg ctacctaata tatgcccgtt
    19741 tattgcatcg cgaaatttga gaagacataa ctcattaaat gaaagggatg caaatgaata
    19801 agtttatcgc tgctgaagct gcggaatgta taggctgtca tgcttgtgaa attgcctgtg
    19861 cggtggcaca caatcaagaa aactggccgc tgagtcacag tgactttcga ccgcgtatcc
    19921 acgttgtagg gaaaggccag gctgcgaatc cggtggcctg ccatcactgc aacaatgccc
    19981 cttgcgttac ggcttgtccg gttaatgctc tgactttcca gtccgatagc gtacaactgg
    20041 acgagcaaaa atgtattggc tgtaaaagat gcgcaatcgc ttgccccttt ggcgtcgttg
    20101 agatggtcga tacgattgcg cagaaatgcg acctttgtaa ccagcgcagt tctggcacgc
    20161 aagcctgtat tgaagtctgc ccaacacagg cgttacgact gatggacgat aaagggttac
    20221 agcagataaa ggtggcccgc cagcgcaaaa cggcagcagg aaaagcgtca tcagtcgctc
    20281 agccatctcg cagtgcagcg ttgctccccg ttaactcgcg taaaggcgca gataaaattt
    20341 cagcaagtga acggaaaaac cactttggcg aaatctattg cgggctggat ccactgcaag
    20401 cgacttatga gagtgaccgc tgtgtttatt gtgccgaaaa agctaactgc aactggcatt
    20461 gcccgctgca taacgctatt ccggattaca tccgtctggt acaggaggga aagattattg
    20521 aagcggcaga actttgccac cagaccagtt ccttaccaga aatctgcggc agggtatgtc
    20581 cacaggaccg tctttgtgaa ggcgcatgta ctttgaaaga tcactctggc gcagtcacta
    20641 tcggtaatct ggaacgctac atcaccgata ccgcgctggc gatgggctgg cgtcccgatg
    20701 tcagcaaagt tgttccccgt agcgaaaaag tggcggtgat tggcgctggg ccagcagggt
    20761 tagggtgtgc tgatattctg gcgcgcgcag gagttcaggt tgatgtcttt gatcgccatc
    20821 cagaaattgg cggtatgctg acttttggca ttcctccttt caaactcgat aaaacggtat
    20881 taagccagcg gcgagagata ttcaccgcaa tgggaatcga ctttcatctc aactgtgaaa
    20941 ttggccgcga tattaccttt agcaatttaa cttctgaata tgatgcagtt ttcatcggcg
    21001 tggggactta cgggatgatg agagcagatc tgccgcatga agatgcgccc ggtgtcattc
    21061 aggctctacc gttcctgact gcccataccc gccagctcat gggattgccg gagtctgaag
    21121 agtatccgct gacggacgtg gaaggtaagc gagtcgtggt attgggcggt ggcgatacga
    21181 caatggattg tttgcggact tccatccgcc tcaatgccgc cagcgtgacc tgcgcgtatc
    21241 gtcgtgatga agtcagtatg ccgggctcgc gcaaagaggt ggttaatgcg cgcgaggaag
    21301 gtgtcgagtt tcagttcaat gttcaaccgc aatatatcgc ttgtgacgaa gatgggcgtt
    21361 taactgcggt gggcctgatt cgtaccgcca tgggtgagcc ggggccggat ggtcgccgtc
    21421 gtcctcgtcc ggtagcgggt tcagagtttg aattgcctgc cgatgttctg attatggcct
    21481 ttggtttcca ggcgcatgcc atgccgtggt tgcagggcag cggaattaaa ctcgataaat
    21541 ggggcctgat tcaaaccggt gacgtcgggt atttacctac ccagacgcat ctgaaaaaag
    21601 tctttgctgg tggtgatgca gttcatggcg cggatctggt tgtcactgca atggccgcag
    21661 gaaggcaggc ggcgcgcgat atgttaactc tgtttgatac gaaggcatcg tgatgaaatc
    21721 gttaattatc gttaatccgg ctgactgtat tggctgccgt acttgtgaag tagcctgtgt
    21781 ggtcgctcac ccttcagaac aggagttgaa tgccgatgtc tttttacccc ggctgaaggt
    21841 acagcgactg gacagcatca gtgcgccggt gatgtgccat cagtgtgaaa acgccccttg
    21901 tgttggcgct tgccccgtgg gggcgctgac gatgggcgag caggtggtgc agaccaattc
    21961 tgcccgttgt attggctgtc agagttgcgt cagtgcgtgc ccgtttggaa tgatcaccat
    22021 tcagtcgttg ccgggggata cccgacaaca aattgtgaaa tgcgatctct gtgaacagcg
    22081 ggaagagggg ccagcctgtg ttgaatcttg cccgacacag gcgctgcagt tgcttactga
    22141 aagagaactc aggcgagtcc gccagcagcg tattgttgcc agcggtgaga atcctctgtg
    22201 aaagaaatct ccgcgctgaa aggcgcggag aatctattag attgcccaac cacccgcgta
    22261 aaatgcgacc agtgcggcag taatgatcac cgtaccaata ttcagctttt gccattcgcg
    22321 tgcaaagacg cggcctacga ccagtgtcac aaagcccagc ataataccgg taacgatatt
    22381 acaagtcaga acgatgaaca cggcacacac caggccagcc atagcgtcaa taaaatcatt
    22441 gaaatccagc ttcgagacgt tacttaacat cagcaaaccc acgtacatca gagcgggtgc
    22501 agtggcgtaa ccagggatca aaaatgacag cggtgataag aacagaatca gcaggaataa
    22561 cgcccccact acggttgcgg tcaaccctgt tttacccccg gcggcggttc ctgccgctga
    22621 ttcgatataa accgctgcgg gcgctgcgcc caccagaccg gagaatattg aacttactga
    22681 gtcactggtc agggctttgc cgccgttgat gatctggttg tctttatcca acaaattcgc
    22741 ctgaccggcg acggcacgga tggtgccagt ggcgtcgaac actgcggtca tcaccaatgc
    22801 cagtacactc ggaagtacag ttggctggag tgcgcccata atatcgaggc tgaaaatcag
    22861 agacttacca tcttcgccag tcaggcttgg catcgccacc agaccgtggt atttcaccgc
    22921 tgggtcaaag attaagccga tgatcgaaat tgcaataatc accaacaaga tcccgccggg
    22981 tacgcgacac ttctccagac cgaagatgac cgccagcccc agtaagctca tcatcaccgg
    23041 gaaggaggta aacgcaccga gcgccactgg caagccttca atcgggtttt tgataaccat
    23101 tcccacaccg ttagcagcaa tcagcagcag aaacaggccg ataccgatac ctgtaccgtg
    23161 agcgatcccc atcggtaaat tacgtaagat ccaggtacgc acaccggtta cggaaatagc
    23221 ggtgaagatg acgcccatca gaaagaccgc gcccagtgcg acaggaacgc taatttgttg
    23281 cccgagtacc agactgaatg cggtaaacgc cgtcaaggaa atcgcgcaac caatcgccat
    23341 tggcaaattg gcccataatc ccatcagcaa cgagccgaag cccgcgacca gacaggtggc
    23401 aacaaacaca gctgcgggag gaaaacctgc tttgcccagc attcccggaa cgacgataac
    23461 ggaataaacc atggccagaa aggtcgttaa gccagccagt acttcctgac gaacggtact
    23521 gccacgagcg gtaattttaa aataattatc cagcgcgccc tgtggctttg gtgcgtccgg
    23581 tgtttgtagg atgtctccag acataatgat gtcctcacga ggatgttaaa gttttattat
    23641 tagttgcgtt cgtacaccag acgaccatca acgtaggtgc ggtagatcga acggtcatcg
    23701 cccaacgtca tcatcacgaa caatttgtcg actaaagaaa cagagttgtc atagcgcagc
    23761 tgctgtagcg gagtggcggt gggttccatc accacgaaat cagcctcttt gccaggtaaa
    23821 aagttgccaa tcaaatcgtc aaggcccaga gatttcgctc cgccgagcgt ggccaggtaa
    23881 aacgcttcat atgccgagag gcgatagcct tgtaattgca acactttgta ggcttcgttc
    23941 agcgtttgca gcatgttgaa agtggttccg gcaccgatat ccgttcccat gcccacttta
    24001 actttcttct gccatgcttt tttcaagttg aataagccgc tgccgaggta aaggttggag
    24061 gtcggacaga aagcaatgct ggatttggtt tcgctgagac gatcccactc tttttcttcg
    24121 agatggacgc agtgagcaaa gacacagttt ttaccggtca ggccgtactg atggtaaaca
    24181 tccagataac catcatggtc aggataaagc gatttcaccc aggcaatttc atctttgttt
    24241 tcacagagat gggtatgtac ccacgtatcc ggatattctt ctttcaggcg ttgcgccatc
    24301 gccatctgtt caggagatga ggtcggggcg aagcgtggcg taatcgcata tagcagacga
    24361 ccatttttgt gccagcgttc aatcagttct ttgctttggt gatagctgct ttcggcagtg
    24421 tcgagcagat aatccggtgc gttacggtcc atcatcacct taccggcaat catacgcata
    24481 ttgatatgac tggcggcttc aaacagcgca tcaacggatt gcggatgaac agtgccaaac
    24541 accagcgccg tggtggttcc gttacgtaaa agctgcttga tgaagaacgc cgacatttcg
    24601 cgggcgtact ctaaatcctc ataacgccgt tcagtaggga aggtgtgttt attcaaccac
    24661 tccagcaatt gctcaccata ggcccccacc atttcacttt gcggataatg gatatgtgta
    24721 tcgacaaagc ccggtactat cagtttgccg cgatagtcgc gcacgcgaat ggtgtcagga
    24781 atttgatgct ttccgttttc ccattcgcca aaccattcca cttttccctg tttaatgagt
    24841 aataaaccat cctcaataaa ccgcagcgca gaggcaatct cttccgggtt atcgaccgta
    24901 cgggtgacat caataaaact gcctcgtacc gcttttaacg tgtgttctcc tgacatcatt
    24961 gactccttta ctgttaatcc atctcttccg ctgaggtaat accaggcaga acgttttcct
    25021 gtcggtagcc accagggagg ataatattta ataaaatcgc agttaacccg ccagcacaaa
    25081 tagggttttc aactaataca taaatagagg ctggcaatat tttaaaaatt tcgggatcgt
    25141 aggagacgcc aagccctaaa cctaaagaag tggcgacaat aagtgtttca cggcgcttta
    25201 aaccgttggt gatgatgatg cgaatccctg cgatggcaat catggaaaac atcaacgtca
    25261 ttgcgcctcc cagaactgcc gagggaatgg tcgtgaagaa gccgccaatc atcggaaata
    25321 agccgaggat aaccagcatt accgcgatgg ttcgcccgac ataacgtgaa gcgacgccag
    25381 tcatctgaat aaccccatta ttttgcgcaa acgtggttaa tggtaatgaa ccgacagcgg
    25441 aggcgataac agaaaccaga ccatctgcca gcacgccgcc tttcagtcgg gactgatact
    25501 cttccccctg aatggggcgg cgggaaacca ttgccgtggc ggtgatatcg ccgactgctt
    25561 ccagcacgct aagcagataa atcgtgccaa ccaccaggaa ctgatggaag ctaaaactaa
    25621 agccgtattt gaatggatgt gggatggtga ttaacggcaa attgcgcata ctgctgaaat
    25681 ccaccatgcc caggcataac gatgcgatat agccgacaca tagcccaata gcgatccctc
    25741 ccatgcgtag caacggactg cgacagcagt taaagccgat caccacaatc aaaaccaata
    25801 aaccaacgcc gagatgttcg taattgccga aggtaccgct gcttttggca gcaaatcctc
    25861 caccaaaatc aataatgccg actttaatca ggcttaagcc gatcatcagt accacaatac
    25921 cgctgacagt aggcgtaata acccgacgta aatagggcag gataaaagaa gagccgacaa
    25981 ccagaaatgc gccaacgaag gagacgccga gaagcgacga catgattaac tcttcgtgaa
    26041 aaccgtcgct tttcatgctg ctgcccagcg caatcatgac cgtaacaaat gaaaaattga
    26101 ctgactgaat tgagagtagg ccagaaccga cgatgccgta gcggtttact tgtaaccagg
    26161 taccaatacc agaggcgatc atcgccattg aaacaagata ggcagttgtt tcagcggaaa
    26221 gctgtaaggc cgcacccacg attaacgcgg gggttaccat cggaacgaaa attgccaaca
    26281 gatgggtaat ggcaccaacg agagcctgat gaaagggagg gcgatcctcc agttcaaata
    26341 taaggtcaga acctgcatgg tttatatcag acatcccttc actcctttta aagtaaattt
    26401 gatcatctga attagtaaaa taaattccca cgaatttggg gcgcaggacg ccttgagaac
    26461 ggcttatctc attttcgcag tcactattta gtcatcgttc aaacagagaa acaggccgcc
    26521 gaagcggcca tttattactt atattttttc cagcgcggtg agtattttct ccggtgtgaa
    26581 atgccattcg cgtaaccaga tgccgcaggc atcgtgaatt gcggtagcaa tcgccggagc
    26641 tgcgccattt acaccgattt ccgagatcga tttcgccccg aacgggccga ctttatcgtc
    26701 gctcggcacc agcacagcgc ggaaatcacg cggaatgtca ccaattttcg gtgcgccgta
    26761 actgcgtaaa tcacgcgtta acgggtgacc ttcggcgtca taaatgatct cttcgctcat
    26821 actgtggccg atagctcgca gggtagcacc gtagatttgt cccaatgcta attccgggtt
    26881 gaccggtgta ccgcagtcca gcaaggcgta gaatttatcc aggcggattt cacccgtacg
    26941 cgtgttgacg gcaacttcag cgaagtttgc gccatacggg aaggcgaaat caggcgtgat
    27001 ataactgcca gttcccacca gtgaaccaaa gccggtgccg gtttcgcctt tatgagcaat
    27061 atccccgaag ctgacttcgc ctttcttgcc gcgcacgacg cccggcgttg ccagttgaac
    27121 atctgccact gcctcaccca acatttgcgc gccgtggaac agaattttct cccgcagatt
    27181 ttccgctgcc aaacgcgccg cgttacctga gaagcaagta ccggacgagg catatgcgcc
    27241 tttatcaaac aacgcatgat cggtatcacc ggagataaca tgcacgtcct gcggtgggca
    27301 atgcagcact tctgctgcca gtttcgtcac cacagtatcc agaccagtac caatatccgc
    27361 accgccagaa tgaacgataa aggtgccgtc cgattccagt ttgatcatgc agttagcctg
    27421 atcgatatcc gggatacccg atttctgcat gataatggcg acgccgcgac cgatgtgcca
    27481 gtcaccattt tgcggtttcg gtgacgacca ttggatcatc tcgcgaccct gacgcaggat
    27541 ctcttctagt gcacagctgg cggcggaagg aacggaggtc ggcgctttac cttcaccgat
    27601 tgcaccgaga attttcagct cttgcccttc gtgtacccgg ttacgttcga taatttccag
    27661 ttggtcgatc tgtaactgtt cagccagttc cgctaatgcc atggtgatag cgaagttacc
    27721 tttcggtgcg ccataaccct gataagcacc atttgggcaa atgttgctgt agtaggtggt
    27781 gacctgaaaa tcgacgttat cgcacggata taacggcagc gacagcgccg gtccgttaca
    27841 tggtacggtg agtgagtggt tgccgtaagg gccagtgttg gcgcggaaat ccatcttcac
    27901 tgctgtcagg cgaccatctc ttttcgctcc cagtttgacg gtgactttcg cgacgtgacg
    27961 agaggtgtta gcaataaact cttcttcacg ggtgtagcgg aacagtaccg gacgcccggt
    28021 cacgcaggtt gcccaggcgc acacttcttc cagcaggatg tcctgtttgg aaccaaaacc
    28081 gccgccgact cgctctttaa tgacatgaac tttatgctgt ttcatgccca cgaggcgcgc
    28141 gacctggcgg cgtaagtgcc atggtacctg ggtggaggcg tggataacca gacgatcgcc
    28201 gtccatacga gtaaagcaga tatgtgtttc agtcgggcac tgctgcgctt gcgttgagtt
    28261 ataggttcgc tcaatgatca catcggcatc ggcaaagcct ttgtccatat cgccaatatg
    28321 accatgaata ctggcggcga tatttttgcg ggggcgagaa ccgatcggga agttaatgat
    28381 catatgctcg ccgcgctggg ctgcatggct gttatcgtct tccagagtat ctggcgcacc
    28441 agcaacatac accaccggtt catcgtgcac gacaggcgca tcttccgcca ttgcttcgtc
    28501 gatcgacatt accggcttaa gcacttcata ttcaacgtcg atgagcttca gtgcttcgag
    28561 cgcaatttct tcactttctg cgaccaccgc agcaacacga tcgccgacgt gacgcatttt
    28621 cttgccgaac atacggcggt caagcggtga cggttccggt gcgctctgac cccccggggt
    28681 atagtagata tccgggcaat tcaggtgagt aataacgtga acgacgcccg gtaaggcttc
    28741 agctttgctg acatccagat gagtaatcag tgcgtgagcg tgtgggctac gtaacatttt
    28801 aatgacgcag gcgtcagccg ttacgcggtc ttcaacatag cagggtttcg cctgcaccat
    28861 tttcgcggca tcagttttag gataatgctt gccaatgact tcaaggtcgt cacggaaagt
    28921 cggagcgata tcaatggtgg cctgcggatt atttttacgt gcaaccgcca gttcaatgac
    28981 ctgatagtat tgttgccagc ctgcatcgcg gctgaacaaa ccagaaagcg cgtcgtcgat
    29041 ctcttcgcgg gtaggtgcgg cgatgcgatc gagaagatcc gtgataatca gagctgcagc
    29101 tggatcgtta taaccagact gcaccacgcc aacatcaacc attgcctgtt gaaccagact
    29161 taactcgttc catttgccca gagattctgc ggtacgaata tctgccttct ctaactgtgc
    29221 ggcaataagc aaggacgcgt taacgatatt accgttaaag attattgcgt cagaaccggc
    29281 aaacccgaag ccatcatcac tgttgcgtac agagtgcatt cccatgttaa acaacagctt
    29341 ttgcacgttt tcgcctggat taacggttag ctcctgaggc gcgccattta aagtaaagtg
    29401 gatgatcata cggcttcctc cccagcctgt tggcagtcgg catacaggtc ggctaccaga
    29461 actcccgtga tatagcgttt ataggccacg ctgccccgca aatcttcctg cgggaaaatg
    29521 gcgttggcga cagcctgttc aagtgcattg ccttccagat tttgtttttc gacatcgtgc
    29581 agacgcagtg ctttactggc gacgccatcc agcgcaattc gcataccgtc gtggtctgtc
    29641 attgcaacgg ctgccgtcac gacggttaaa cctgcctgag aacggctaat tttgcgggtc
    29701 gcacaggtgc gatacggatc tttaatgata atttcggtta gcaggcgatc gcatgggctg
    29761 gccaggtagt cctcgattga cagcgtttcg ccgttgccaa aaaccagttc agcatccagt
    29821 gccagcagga caggaagcag caccgactct tcctgccggg cggcgatttc accaccaatg
    29881 gtcgactgat tacgaacatg gcgtgagtaa acaaaaccga gggcttcacg cagtgctgca
    29941 ggaataaatc gcgcatcacg cagtggctgc aagcgagaca ttgcaccaat ccgcagtgca
    30001 ccgttatccc agtcaaccca gtccagttcc agatcctgta aggaaatggc aatcttttta
    30061 tcggtacggg ttggtgtagc gttgagtttg ctgcccccgg cgaaccagac ggcttcatcc
    30121 tggtagcggc gcttcagttc cagcgcctgt tcgacagagt cgggcctgaa aaattgttca
    30181 atcatcttct ttccttattt aaccctcttc cctgcgggaa gagggcatct gtcatttatg
    30241 ccagcgtatc catccgacgc cacatactgg cagcggcttt tctggcttgt gcataaatgg
    30301 aatcgcaatc aaagttaaac tgacggtctt catagaccat cacgccattg accatcacgc
    30361 tgtgaacgct gcctgagccc ataccgaaag cgatatgccc ggcgatattg tctgccagca
    30421 gcggcgtcgg cgagttgtaa tcgcaaatgg ttaaatcggc tttgtaaccg gcttccagaa
    30481 gaccaaattt cgcggcaaag ttgcggctca tcagttcgtt accgttagtc agggctttgg
    30541 caaaactgtc aggccacagc ggaccacccg catcgcgatg tttaaagaag gcaaatttca
    30601 tctcttcaaa catgtccgaa ccaatgccgt ccgttcccaa cgccagattg cggatgtcgc
    30661 taaggtgatg gttgtagccg acatggttgt tcatgtttga acgggcgtta tgcaccagga
    30721 acgcatcgcg ctgattgagt agggcgatgt catctttcga caagtacagc ccatgagcga
    30781 ccagcgtttt gctgtcgatg agatcgaatt gcgccagtcg tgccagcagg tctttgccgt
    30841 accagtggtg actgtaggaa acgtcgtaaa ggtcttccgc agcgtgaata tgcaaaccac
    30901 gacctgtggc tttcacggct tcacgcagca tctccagacc ggcatctggc acggtaaacg
    30961 gcgcgtgagc accgatatgt gcttccacca gatacggctc ggttgctgct ttcttcgcct
    31021 catcaatctg acgggcaaag cggatgtttt cttctacacc ttcctgcaac tctttgatgc
    31081 cgttgttacg gtcagtagtt tcaaaacagg tcattgcgcg caggccaact tttaaaaatg
    31141 cgtcgcgcaa tgtggagagc gacccgccga tatacgctgg agaggcatgg tgatcgataa
    31201 ccgatgtaca tccgctctta atcgcttcca gggaacaaat cagcccgctg taatagagcg
    31261 actcttcatc aagggcgcga tcgagccgcc accagagatt tttcagcgtt gagatgaaat
    31321 ccgggcaggg ggcgatgttt gccataattc cgcgggaaag ccccgagtaa aaatggttgt
    31381 gcgagcagac aattcccggc atcacaatcc gaccatgcat ctctttgtag ctggcatcgg
    31441 ggtagcgttg cgtcagggca tcgccgatag cgacaatcac atcattttcg atagcgatat
    31501 caacggcttc ctgcactttc gccgggtgta actgcactgc ggtgacattc ttcagaatca
    31561 acatgattac acctccacgc ggcccagcag ataatgatga tgctggtgga catggctgat
    31621 gatgcggcac atatcgttca gctccggtgg tacgttgttg aactgacctt cgctgtcgat
    31681 gtttaacacc cagctttggt tattcagacg tactcgtacc cggcaatctt ccacaaggaa
    31741 gcctgggttg ctgctgttat caaagtcttg cgacaggctg aagacggtga ttttgtcttt
    31801 gtatggttta ccgttccacg gacagaactg agcgcagttg ccgcattcgt tacagtaagc
    31861 gtcgaggtgc agcgtctgga aacggttctg gaagcctggg accgcaatgg agacgttggc
    31921 gcggttcggg cagacatcca cgcacttgct gcaaacgtag ttacattcaa ggcagcgagc
    31981 agcttcctgc gcgacaaacg catcacgatc gtcactgttc accagcgtga tagagatatc
    32041 gcctttacgt tgatagattt ccgccggatt gacgttgttc cagtatttat cgttctggtg
    32101 ggaacggata ttttcccgac taaggatggc atcggtcgcc cgacgcgcgg ttccgacagc
    32161 agcgacaatg gaggatggtc cgcgctgtac gtcgccgatc ataaagacgt cagtcagacg
    32221 agtttcgcca ttatggtcga cgtctggcca gccgtttttg tccagcggca cgcccatcgc
    32281 attcagggct tcagtatcct gctgttcacc aatggcggtg atcaggctgt ctacatgcag
    32341 tgttactgtt tcgttggttt caaccggacg acgacgacct ttctcatccg gttcgccaag
    32401 cgacataacg cgcaaggtta aggtgccatc agcatcgaaa cgttccggat tattcaggaa
    32461 acggaactcc acgccgtcgt gcaacgcttc ttcatactct tcgcgccatg cgggcatctc
    32521 ttgcagtgaa cgacggtaaa cgatcgttgc tttttttacg cctggaacgc gtaacgccgc
    32581 acgagcgcag tccattgcgg tgttacccgc cccgacaacg accacatgtt tgcccagctt
    32641 gagcgctgta cccttgttgt attcacgcag gaaggggagt gatttccaga cattttggtt
    32701 gtcgcccgcc agtttcacac cgctattttt atcagtgccg gtggcaatca gaacatagtg
    32761 gaagccctga ttttttaact gttcaacggt caaatcgggt gagcagccat actcaaattt
    32821 cacgccgtga gcggcaacaa aatcgatatc gtgctgaatt aactctgcag gaatacggaa
    32881 ctgagggatg atatttttca ccacgccgcc cgcattggct tcgcgttcaa acagcgtaac
    32941 cggatggccc gctctggcaa ggaagtaacc tgcagccaga cccgccggac ctgcaccaat
    33001 cacggcaacc ggatggcgtg aaccagaacc ggctggtttg tgccagcgtt gcttatattc
    33061 atcccaacct ttttccagcg cgactttttt cagttcgcgg atattcagtg cactgtcgta
    33121 atccaggcgg gtacagttgt attggcactg gtgatcgcaa atatgaccgg taatggcggg
    33181 cagggcgttg cgttggtaga taagttccag cgcgtcggca tagcggtgtt cgccaagcag
    33241 acggatgtat tccggaatat cttgcttgat cgcgcaggca gtaacacagg gggcaacgta
    33301 gcagtcggtc agcggcaggt cttctgccac ttcaatacgc tcttctggct tccagtgttt
    33361 ctgggtgtat tccatggtta gcgcgtctgc tgccagtctg ttcagtcgtt cgacgtcaac
    33421 atggtcaagt ccccaggcgt cggagccttc cagctcgcgc atgcaggcac ttaaacgcag
    33481 atagccacca ggtttcagca ggtcggttgc catagtaata gggcgaatac ctgtatcaaa
    33541 aatatcgcgg atagtcagct gactggcacc accggaataa gaaatgggca gtttgccgtc
    33601 aaaggcgcga gagagaactg ctgcaacatt gatggagagc gggaacagcg cacggcctga
    33661 catatacatc tcttcaccag gcagtgcgcc tttattgttg atggtgccga gagtgttagt
    33721 cagttttacg ccaaagccga gtgatttttc ttttgccagt gccatcaggc gttccagcat
    33781 ttccagtgct tgcgtcagct tgaggtcgtg atcaaatgac tcttctttta agcctatgta
    33841 accgaaaccg cagacatcga ggatctcacg aacacgcgcg taccccagta aggtcgggtt
    33901 aagtttcaca aaggtgttga gccctttttc ttccagcatg tagcggcaaa tggcttcgat
    33961 ttcatgcgga ggacagccgt gcatggtgga gagggtgacg ccatgcacca tactggtggg
    34021 gatgcgagcg ggtaaggctt gcaagctttc gcgtttttcc tgcaatccgt ggcgggctaa
    34081 aaatgcgtca tcctggagta atttattcag cgtatcgcga tattgcgcga atttcggatg
    34141 gtcagatgcg tccatcatat tgtcgatgaa ctgttgcatt ggcggttgct taataccttc
    34201 gaggttgtaa ccgacgctca tattaaagat gaacgattta ccggaatcag aaggttggaa
    34261 catcgcttcg agaaggtgca gggcaaacca ggctttgagg tattcatccc aggctttaag
    34321 cagggtaaat tcggtagacc attcggtgtt aaagcactcg tcttcggcat caatacaggg
    34381 cttttccagc tccaggcggt caagaatttg gacggttttt agttcgatga aacgtccgcc
    34441 agtcagccag gaagtgacaa tattttgcgc gagctgcgtg tgcgggccag cggcagggcc
    34501 gacgggagtg gcacaggttt caccgaagac gctaacagtt ttacctttta caggtgagta
    34561 aaactgttgc tcgggaatac caaagattga gcgttgttgt tggtattcat caaatatgcg
    34621 cgtcaaaagt tcctcaaacg gaatgggacg cataatatcc cccataaccc tctccttagc
    34681 ttatacagat atcattgagt gaccggtgtt gagaaatccg gtggtttagg gggatagagc
    34741 aacaatcgca ccactcttgc caggtttggc gggaaatata aaaaaatgtg aggcggctcg
    34801 aaacttaagg gctttttgtg aatgaactca caggtgctaa atgagaatta aaatcactcc
    34861 tgaaaattat tattgccagc ccatatatca ctttttctct tttctcaaaa tcagtgattc
    34921 cttatcatta tgataaagag tgctggaggg attaaatatt tgaacgcaat cgtttaacta
    34981 cgttttttaa attttagtat accttttttg cgctgtaata aagtcgtccg gagtatcaat
    35041 atctaaaatt atttctgcat tttctacttc gacggaataa tgagctccca taaggagtgc
    35101 ctgacgcata ttggtgatat tggggcgctt gattgcctgc atcaggcatg gttttgacac
    35161 taaaatcgga tggccgggga tgccattatg gagtggcagt attgcgccat catttcgtaa
    35221 cgaccagatt tttctgaaaa tatcgatggt gagggttggc atgtcaccgt gggtgagaaa
    35281 acaatgttct gtttgcactg cgggtactgc ggcctttact gatgttagta aaccctgcgc
    35341 ataatctggg ttgtggataa tagtgatatt gctctggttc gcataacgtt cgtgcagttc
    35401 attaccacga tagccggtga ctaaaataat tcggctacaa aactgcaacg catttttgat
    35461 acttgtatca agaattgtcc cctgttgcca gggtaacatc attttccatt gtcccattct
    35521 tgatgataat ccggcagcgg taattataca gtcgatggct gacatagtgg ttccattatt
    35581 tataggggct aataacgcct gataattgcg ttacttcctg atggattaag ttggggataa
    35641 tttatcccag agaggtcata aagactcgtg aaaagtatag ttgacccatc ggcattattc
    35701 attgatttag gtgcgcagaa acgtcccact gtgatttctg ttgttggtgc tggggggaaa
    35761 accagcttgc ttttttggct ggcagaactg ctccaggcta gcggcaggcg tgtgttaatc
    35821 acgacgacaa cacatatgtt tatgccaacg tctcactggc ccgtggtttt ctgtcgtgat
    35881 cccgccatgc ttcctcatgc gtctcttaca tcccccattt cattttgttt tcacagctgg
    35941 aaagcgaacc agggaaaagt gcagggattt acgccagaag cgattgatgc actggtgcaa
    36001 cgaccagagt gtgacgtaat tctcattgaa gcagatggct cgcgtgcaat gccgttaaaa
    36061 gcgcctgatg agcacgaacc ttgcataccc aaaagcagtt gctgcgtgat tgctgtgatg
    36121 ggaggacata ttttgggcgc gaaagtgagc acagaaaatg tccatcgctg gtcgcagttt
    36181 gctgatatta ccgggttaac acctgatgca accttgcaac tgagcgatct cgttgcgctg
    36241 gttcgccacc ctcagggggc gtttaaaaac gtaccgcaag gttgtcggcg agtctggttc
    36301 attaaccgtt tttctcaatg tgagaatgcg attgcgcaaa gcgagctcct tcaaccgctg
    36361 caacaacacg acgtagaggc aatctggctg ggcgatatac aagaacatcc tgcaatcgcg
    36421 cgcagatttg tgaattagcg gcccgggtat tccgggttac tgaaaggata tccattgagg
    36481 ttggtatgaa tattttcaca gaggctgcaa aactcgaaga gcaaaattgt ccgtttgcga
    36541 tggcgcaaat tgttgatagc cgaggctcga ctccccgcca ttctgcacaa atgttagtgc
    36601 gcgccgatgg ttctatcgtc ggtacaattg gtggcggaat ggttgagcgg aaggtgattg
    36661 aagagtcact tcaggcattg caggagcgta agccgcgatt attccatgga cgtatggctc
    36721 gtaacggtgc ggatgctgtc gggtcagatt gtggaggtgc aatgtcagta tttatcagcg
    36781 tccatggtat gcgtccacgt ctggtgttga ttggcgcggg gcatgtcaac cgggcgatag
    36841 cccagagtgc ggcgctatta ggatttgata ttgccgttgc cgatatttat cgcgaaagcc
    36901 tcaatcctga actattccca ccatcaacca cgcttctcca tgctgagtcg tttggtgcgg
    36961 cagtggaagc actggatatt cgccctgata attttgtcct gattgccacg aataatcagg
    37021 atcgtgaagc cctcgacaaa ctcattgaac agcccattgc atggttgggg ttgctggcaa
    37081 gtcgtcgcaa ggttcagctt ttcctgcgtc aattgcgtga gaaaggcgtg gctgaagaac
    37141 atattgcccg tttacatgcg cccgttggtt acaacatagg tgcggaaacg ccgcaggaga
    37201 tcgccatcag cgtgctggca gaaatattac aggtgaaaaa taacgcgccg ggtgggctga
    37261 tgatgaaacc ttctcatcct tccggacacc agctggtggt gattcgcggt gcgggagata
    37321 tcgccagtgg tgtggcgcta cgtctgtatc atgcgggttt taaagtgatc atgctggaag
    37381 tggaaaaacc gacagtgatt cgttgtaccg tggcgtttgc ccaggccgtg ttcgatggcg
    37441 aaatgacggt cgaaggcgtc actgctcgcc tggcaaccag ctctgcggaa gcgatgaaac
    37501 ttaccgaacg cggattcatc cctgtgatgg tagatcccgc ctgttcattg cttgatgaac
    37561 tgaaaccgct ttgcgtggtg gacgctattc tggcgaaaca gaatttgggg acgcgggcag
    37621 atatggcacc agtaacaatc gcgcttgggc cgggctttat tgcagggaag gattgtcatg
    37681 cggtaattga aacaaatcgc gggcactggc tcggtcaggt gatttactct ggttgtgcgc
    37741 aggagaatac cggtgttcct ggcaatatta tggggcatac cacccgacgg gtgatccgtg
    37801 ctcctgctgc aggcattatg cgatccaacg tgaaattagg cgatctggtg aaagagggcg
    37861 atgtgattgc ctggattggt gagcatgaaa ttaaagcacc gttgacgggg atggtgcgtg
    37921 gcttgttgaa cgacggcctg gcagtggttg gtggttttaa aattggtgat atcgatcctc
    37981 gtggtgaaac cgctgatttc accagcgttt ctgataaagc ccgggcgatt ggcggcggcg
    38041 tacttgaggc gttaatgatg ttgatgcatc agggcgtgaa agcgacaaaa gaagtgctgg
    38101 aagtggctta aatgaaaatg gccggatggt attacccacc cggccttaaa attactgaca
    38161 aatcaccgtt ccggttttgc cttcaatccc ttctttcgct ttgctcaata cggtaatcag
    38221 tgcttcacga cctgcgcgag agcgagcaaa cgaggcggca gcttccactt taggcagcat
    38281 agaacctttc gcaaaatgac cttcttcgat aaagcgttca gcatcactta atgacaggcg
    38341 atcgagccac tgttcattct ctttaccgaa gttaatggcc actttttcta ccgcagtcag
    38401 aatgatcagc atatcggcat caatcatttc tgctaaacgg gcgctggccc agtctttatc
    38461 gataaccgcg ctggcaccac gcagatggtt accttcacga ataacaggga taccaccacc
    38521 gccaacggtg atcaccacct ggcctgcatc taccagagct ttaaccgttt ctttttcaat
    38581 gatatcaacc ggttttggcg aggcaacaac acggcgatag ccgcgaccag catcttcttt
    38641 cagagtgtaa ccctgttttg tcagttgctc tgcttcttgc tcggtaaaga acgaaccgat
    38701 cggcttggtc gggttgagga atgccggatc gttagcgtcg acttcgacct gagtaaccag
    38761 cgttgctaca ggtttattaa tgccccgaga aagcagttct tctcttaacg cgttttgtag
    38821 atcgtaacca atataaccct ggcttaaggc aacacagaca gacatcggca gcattggcga
    38881 gtgtgcttca gtttttgcgg cagcttcaaa ggcctggtta atcatgccca cctgtggccc
    38941 gttaccgtga gtgacgatga cctcatgtcc ctgggcaatt aaatcaacaa ttgcctgaga
    39001 agtaattttt accgctttca tttgcccggc cagatcatcg cccagcgcat tcccgcccag
    39061 ggcgagaaca attttcttac tcatttttcc tcactcatca ttcttgattg tgtatagatc
    39121 cctggcagtg gttagagcac gggagggaca aacggtttgc gtcgcaggaa acgacctcgc
    39181 ccggctttgc ctgtaaaggt gccgtcacag aaaatcgttt caccacggga taaggttctg
    39241 acaatcgcgc cctgacaggt aaaaccctcc catggcgagt agtcggcgtt gtcgtggaga
    39301 tggcgatgct gaatttgttg gctctgacgt gggtcgataa tcaccacgtc gccatcggaa
    39361 ccgggcgcta ataatccttt ttgcggccac aggccaaaca gcctggcggg cattgcgctg
    39421 gttaattcaa caaagcgttc cggtgttata cgtcccgtca tcacgccact ggaaaacagt
    39481 aactgcatgc ggttttccac gccgggtaag ccatttgggc agcgactgaa atcgccttta
    39541 gaaatttgca ggcgttgagc cattgagaac gtgcagtgat cggtcgccac cacgtcaatc
    39601 gcaccatcgc tgatgccaca ccacagtttg tcctgctcgc gtatgttacg cagcggtggg
    39661 ctaagaatga acttcatgcc atcttctgta tcgtaactgc gttcgtccaa caggagatat
    39721 tgtgggcagg tttcaaccca gactggctgg tgattcgcac gggcaagacg cagataatcc
    39781 agacctaagc cgttagacag gtgcacgata tagagcgggg cgttaccggc aatttgtgcc
    39841 aggttaatca tgcgggcgat ggcttccgct tcgcattcca gagggcgact caaggcgtga
    39901 tagcgcggcg cggttaaccc tgcggcgata aactccgccc gcttgctggc gatagccgca
    39961 tcattttccg ggtgcacggt ggtcagcgcg ccggattcat gcagacggcg taatgcctgc
    40021 aaaacctcgt catcgttgag tttgtattga taggttaaat agagtttaaa actgctcagc
    40081 ccttcctcga ccatcatagg gatttcgtcg aggatggcgt gattaatgtg ctggatcaca
    40141 ccatgaaagc tgtagtcgat gaccgcctta tgggcggcat agccacgata aacctccagt
    40201 tgatggcgta accgacaccc gtttgggcca aatcccatat ggtcaataat ggttgttgta
    40261 ccgccacacg cagctgcgcg ggtaccggta aaaaaatcat cacaactgcg cgcgatgccg
    40321 acatcaatat tgaaatgcgt atggacatcc acgccgccag ggaaaacgta acagccagtg
    40381 gcatcaattt cttcatacgg gagctgcggc gaaatattgt tgcccaactg gcgaacaatc
    40441 ccgctttcaa tcagcaaatc ctgtttggct tgtccatctg cgttaacgac agtgccgttt
    40501 ttgatcaata cgcgcatagc aaactccaaa aaccctccgg cgaaccggag gggaataagt
    40561 gaagagtaat tattcggttg ccagccagct taacgggatt gcagcgtaca tcgcagcaca
    40621 ggtcaccagg tgagatttcc aggttttttc gttaggtgca tgcgcttcag gttctttacc
    40681 cgggccaaag ccgatcaccg gaatgccgtg acggcccatg atagatacgc cgttagttga
    40741 gaaggtccac ttatcaacaa ccggcgcttt gccaaacagc ccttcgtagg cattcaccag
    40801 tgctttaacg gtgaagtgat cttcttccac tttccaggtc gggaagtagc attcggttgg
    40861 gtaaaccagg ccagtccagg acggacggtc gtagttgtac atagaaacaa ccgcgttagc
    40921 tttctgtact gcaggcaggg cgcggatttc gtccagcgcg ccttcccagg tttcgcccca
    40981 ggtcagacga cggtcgattg aaactgcgca gctgtctgct acagcgcaac ggcttgggga
    41041 tgtgaagaag atttcagaaa cggtgagggt gcctttgccg aggaattcgt cataacccag
    41101 acgttgggag agttcttgta attcgccaag aatcggaccc attttgaaaa tggcgttatc
    41161 accgcgttct ggtgcagaac cgtggcagct aacaccctga acatcaatac gaatttccat
    41221 acgaccgcgc tgaccacggt atacctggca gtcggttggt tcggtactga ccacaaattc
    41281 cgggcgaatg ccggattgtt caataatgta ctgccagcac agaccgtcgc agtcttcttc
    41341 ctgcacagta ccggtaacca gcagggtata ttcatcttcc agaccgaggt ctttaatgat
    41401 tttaccggca taaaccatag atgccatgcc gccttcctgg tcggaagtac cgcgaccacc
    41461 gattagctca tcagtttcca tgccttcgta cggatcgaag tcccagttct tgatgttgcc
    41521 aatgccgacg gtatcgatat gagcgtccat tgccaccaga cgcgggccgt ggccgatata
    41581 accgagaacg ttgcccatcg ggtcgatttc aactttatcg aagccgactt tttccatctc
    41641 ttctttaata cgatgtacta cgcgtttttc gtcgcagctt tcactgggaa tagcaaccat
    41701 gtcgcgcagg aagcgagtca tgtccgcctg gtaatctttt gctttttcaa gaatcagttt
    41761 gaatggaata ttcttagcca ttatttttct ccagattttt tactccccgt tttgcaggga
    41821 gtagttccaa tagggtgatt aaggtgctac agcgtgtttg ccttcccaga caacttcgcg
    41881 gtagtgcttc acgtcggtgt cgccttcagt actgataacc agcactacgg catctttgtt
    41941 cagcgccagt ttttccatca ggctttgacg ttgcgggtga taatgaaccg ctgcgagaac
    42001 gcccaaaccg acagcgccgg attcaccgga gatgatgcgc gggtcgttgc cgtacggatt
    42061 acccagcacg cgcataccta atgcggcaac gctgtcctgg caggagatga attgggtggc
    42121 gcagttacgt aggatttccc agcccagcgg gttaggttcg ccacaggcca ggcccgccat
    42181 gatggtggcc atatcaccgc caacgttgac gatgtcgcct ttgacgccgg agcgataaat
    42241 acagtcagct ttgtcaggtt caacaataat gctgtgcaga ttttgcgggc tgtagacgtc
    42301 gaccagataa cccagcacac caccggccat tgcaccgaca ccggcttgca gcagaacgtg
    42361 cgtcggggtt acgcccattt cacgcatttg ctcgacggct tcatctgcca gggttgcgta
    42421 gccttgcatg atccaggttg ggattttggt gtaaccttcc catgccgtgt cctgtaccac
    42481 ttcccagccg tgctgctgcg cgtgttgcat ggtcaggcga acggtatcgt catagttcat
    42541 atctgtgacg atgcactcgg caccgaggtt cagaatggcg tcaacgcgtt cctgagcaga
    42601 acctttcggc atgtaaatca ccgcattctg tccgagttgc tgtgctgccc acgccacacc
    42661 gcgcccgtgg ttgccgtcgg tggtggtcgc gaaagtcatt ttttcgccga tggcattttt
    42721 caggtgctca aatgacagcg tttcgatatc aagatgatat ttttcacaca ataattgagc
    42781 gatggcgtac gcaccgccaa gcattttgaa ggcgttcaga ccgaatcgtt ttgattcgtc
    42841 tttgacgaga attttcttca caccaaaaag gtttgcgaga tcgtccagcg cacaaagcgg
    42901 tgttgggcga taaccagcta ttttctggtg gaactggcgc gccagtttgg cctggctttg
    42961 cgaaaagagc ggtgatgttt cgccgttgaa aaatttgtta tcggcgatat caatcttcaa
    43021 tgagaaaacg gacatatcct atccttttgg cctctggaaa tagagggccg accagctgat
    43081 cggcccgagg ggttatttca cgcgttcttg cgcttctttc agcagttgct cgagcagtgc
    43141 acctggtttg gcgtatttac gggacaggat catcgcggcg atgatataag gcttccagct
    43201 ggcttctttg taggtagcga tacggtattt ttcgaatacg ccttcagtca cttcaccttc
    43261 tttgcaggat acgccgctga tatcagctgg caggcagtgc atgtacaggg cttcgccatc
    43321 acgggtcagt tccatcatct cttcagtaca atgccagtct ttgtgttgcg cgttctgtgc
    43381 cagacactgt ttttccagtg cttttaagcc ttcgtggtcg ttcgcacgca gcaattcagt
    43441 acgctcttcc atcactttgt aaggtgccca tgacttcgga taaacgatgt ctgcgtcttt
    43501 gaaggcttct tccatgctgg tgacctgacg gaagctacca ccggaggctt tagcattgtt
    43561 tttcgccact tcaaccacat ccgggatcag gtcgtagcct tccggatggg ccagggtgac
    43621 atccataccg aagcgagtca tcagaccgat gatgccttgt ggtacagaga gcggtttgcc
    43681 atagcttgga gagtaggccc aggtcatggc gattttttta cctttcaggt tttccagtga
    43741 accaaagtgt tcacgtaacc acgcgaggtc agccattgac tgagtcgggt ggtcaatatc
    43801 gcattgcagg ttcactaaag ccggacgctg tggcagtaca ccctgcttgt aaccgtcatc
    43861 aagtgcagcg ccaacttcac gcatataggc gttgcctgcg cccagataca tatcgtcgcg
    43921 aataccaata gcgtcggcgc agaaggagat catattggcg gtttcacgca cggtttcgcc
    43981 gtgagcgatt tgtgatttgc cttcgtcgag atcttgttgt gcaaggccga gcaggttaag
    44041 cgcggaagca taagagaagc gggtacgggt ggagttgtcg cggaatacgg aaatacctaa
    44101 tccactatta aagactttgg ttgagatgtt ttcagcacgc agtgctttta atgctgcggc
    44161 aacgtccagt acttgtttca gttcatctgg cgtctgttcc cacgttaaca aaaaatcttt
    44221 ctcgtgaagg tgagaggtca gcgaattgat atccttaatc agctcattaa cagttttcat
    44281 cataaagtcc cggtagtgga atgacaggca aagtggttta tttcactgtg acttgccaaa
    44341 gtgattggtg ttttcacagc tatacctggg actaacaata agtgtgccag aattatccgc
    44401 gcggctgcaa aagactaatt tgacggataa tgtgtggggt tgatcacaaa ttgatgccag
    44461 cttctggttg tttgtaaaat acgaaaacgt ttggcttttt ttatcgcgtt atttatttgc
    44521 tgctaagtat cagaaataag ataaaggggc aggctatctc tcttcagttt gataaactca
    44581 ccgccgtaat tctca
//
LOCUS       DBANUU010000025        42294 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000025 DBANUU010000000
VERSION     DBANUU010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.025"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            182..808
                     /locus_tag="ACIE68_001309"
     CDS             182..808
                     /locus_tag="ACIE68_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFX5907655.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            829..2931
                     /locus_tag="ACIE68_001310"
     CDS             829..2931
                     /locus_tag="ACIE68_001310"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFX5907656.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            2944..3582
                     /locus_tag="ACIE68_001311"
     CDS             2944..3582
                     /locus_tag="ACIE68_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFX5907657.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3646..4314
                     /locus_tag="ACIE68_001312"
     CDS             3646..4314
                     /locus_tag="ACIE68_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFX5907658.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4311..5096
                     /gene="phsC"
                     /locus_tag="ACIE68_001313"
     CDS             4311..5096
                     /gene="phsC"
                     /locus_tag="ACIE68_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFX5907659.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGGLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5100..5912
                     /gene="ydhT"
                     /locus_tag="ACIE68_001314"
     CDS             5100..5912
                     /gene="ydhT"
                     /locus_tag="ACIE68_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFX5907660.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(5924..7528)
                     /locus_tag="ACIE68_001315"
     CDS             complement(5924..7528)
                     /locus_tag="ACIE68_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFX5907661.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRAPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7654..7959)
                     /locus_tag="ACIE68_001316"
     CDS             complement(7654..7959)
                     /locus_tag="ACIE68_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFX5907662.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8067..8143)
                     /locus_tag="ACIE68_001317"
     tRNA            complement(8067..8143)
                     /locus_tag="ACIE68_001317"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8107..8109),aa:Val,seq:gac)
     gene            complement(8148..8224)
                     /locus_tag="ACIE68_001318"
     tRNA            complement(8148..8224)
                     /locus_tag="ACIE68_001318"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8188..8190),aa:Val,seq:gac)
     gene            8532..9788
                     /locus_tag="ACIE68_001319"
     CDS             8532..9788
                     /locus_tag="ACIE68_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFX5907663.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9829..11202)
                     /gene="mdtK"
                     /locus_tag="ACIE68_001320"
     CDS             complement(9829..11202)
                     /gene="mdtK"
                     /locus_tag="ACIE68_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFX5907664.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIICSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            11417..12058
                     /gene="ribE"
                     /locus_tag="ACIE68_001321"
     CDS             11417..12058
                     /gene="ribE"
                     /locus_tag="ACIE68_001321"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFX5907665.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12098..13246)
                     /gene="cfa"
                     /locus_tag="ACIE68_001322"
     CDS             complement(12098..13246)
                     /gene="cfa"
                     /locus_tag="ACIE68_001322"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFX5907666.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13537..14748)
                     /gene="punC"
                     /locus_tag="ACIE68_001323"
     CDS             complement(13537..14748)
                     /gene="punC"
                     /locus_tag="ACIE68_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFX5907667.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14861..15793
                     /gene="punR"
                     /locus_tag="ACIE68_001324"
     CDS             14861..15793
                     /gene="punR"
                     /locus_tag="ACIE68_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFX5907668.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15790..16815)
                     /gene="purR"
                     /locus_tag="ACIE68_001325"
     CDS             complement(15790..16815)
                     /gene="purR"
                     /locus_tag="ACIE68_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFX5907669.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17114..17203
                     /gene="cydH"
                     /locus_tag="ACIE68_001326"
                     /gene_synonym="ynhF"
     CDS             17114..17203
                     /gene="cydH"
                     /locus_tag="ACIE68_001326"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFX5907670.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17369..18538
                     /locus_tag="ACIE68_001327"
     CDS             17369..18538
                     /locus_tag="ACIE68_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5907671.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18773..19354)
                     /gene="sodB"
                     /locus_tag="ACIE68_001328"
     CDS             complement(18773..19354)
                     /gene="sodB"
                     /locus_tag="ACIE68_001328"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFX5907672.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19482..20297)
                     /gene="mepH"
                     /locus_tag="ACIE68_001329"
     CDS             complement(19482..20297)
                     /gene="mepH"
                     /locus_tag="ACIE68_001329"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFX5907673.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20631..20978
                     /gene="grxD"
                     /locus_tag="ACIE68_001330"
     CDS             20631..20978
                     /gene="grxD"
                     /locus_tag="ACIE68_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFX5907674.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21029..25645)
                     /locus_tag="ACIE68_001331"
     CDS             complement(21029..25645)
                     /locus_tag="ACIE68_001331"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFX5907675.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEKA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIEEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DTGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25738..26385)
                     /gene="rnt"
                     /locus_tag="ACIE68_001332"
     CDS             complement(25738..26385)
                     /gene="rnt"
                     /locus_tag="ACIE68_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFX5907676.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPTAEEV"
     gene            complement(26488..26895)
                     /gene="gloA"
                     /locus_tag="ACIE68_001333"
     CDS             complement(26488..26895)
                     /gene="gloA"
                     /locus_tag="ACIE68_001333"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFX5907677.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26976..28073)
                     /gene="nemA"
                     /locus_tag="ACIE68_001334"
     CDS             complement(26976..28073)
                     /gene="nemA"
                     /locus_tag="ACIE68_001334"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFX5907678.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28110..28709)
                     /gene="nemR"
                     /locus_tag="ACIE68_001335"
     CDS             complement(28110..28709)
                     /gene="nemR"
                     /locus_tag="ACIE68_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFX5907679.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            28812..29051
                     /locus_tag="ACIE68_001336"
     CDS             28812..29051
                     /locus_tag="ACIE68_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFX5907680.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29100..29996
                     /locus_tag="ACIE68_001337"
     CDS             29100..29996
                     /locus_tag="ACIE68_001337"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFX5907681.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSTEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            30077..30598
                     /gene="sodC"
                     /locus_tag="ACIE68_001338"
     CDS             30077..30598
                     /gene="sodC"
                     /locus_tag="ACIE68_001338"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFX5907682.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30599..32611)
                     /locus_tag="ACIE68_001339"
     CDS             complement(30599..32611)
                     /locus_tag="ACIE68_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFX5907683.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAITNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32611..33468)
                     /locus_tag="ACIE68_001340"
     CDS             complement(32611..33468)
                     /locus_tag="ACIE68_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFX5907684.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVKAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33471..33707)
                     /locus_tag="ACIE68_001341"
     CDS             complement(33471..33707)
                     /locus_tag="ACIE68_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFX5907685.1"
                     /translation="MKFMLNATGLPLQDLVFSASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33908..34342
                     /gene="slyA"
                     /locus_tag="ACIE68_001342"
     CDS             33908..34342
                     /gene="slyA"
                     /locus_tag="ACIE68_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFX5907686.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34389..34856)
                     /gene="slyB"
                     /locus_tag="ACIE68_001343"
     CDS             complement(34389..34856)
                     /gene="slyB"
                     /locus_tag="ACIE68_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFX5907687.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35130..36239
                     /gene="anmK"
                     /locus_tag="ACIE68_001344"
     CDS             35130..36239
                     /gene="anmK"
                     /locus_tag="ACIE68_001344"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFX5907688.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36337..36666
                     /gene="mliC"
                     /locus_tag="ACIE68_001345"
     CDS             36337..36666
                     /gene="mliC"
                     /locus_tag="ACIE68_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFX5907689.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36725..37381
                     /gene="pdxH"
                     /locus_tag="ACIE68_001346"
     CDS             36725..37381
                     /gene="pdxH"
                     /locus_tag="ACIE68_001346"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFX5907690.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37510..38784
                     /gene="tyrS"
                     /locus_tag="ACIE68_001347"
     CDS             37510..38784
                     /gene="tyrS"
                     /locus_tag="ACIE68_001347"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFX5907691.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38843..39706
                     /gene="pdxY"
                     /locus_tag="ACIE68_001348"
     CDS             38843..39706
                     /gene="pdxY"
                     /locus_tag="ACIE68_001348"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFX5907692.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39750..40355)
                     /gene="gstA"
                     /locus_tag="ACIE68_001349"
     CDS             complement(39750..40355)
                     /gene="gstA"
                     /locus_tag="ACIE68_001349"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFX5907693.1"
                     /translation="MKLFYIPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGFEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40461..41963)
                     /gene="dtpA"
                     /locus_tag="ACIE68_001350"
     CDS             complement(40461..41963)
                     /gene="dtpA"
                     /locus_tag="ACIE68_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFX5907694.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT        10660 a        10427 c        11079 g        10128 t
ORIGIN      
        1 cacattattt tttctcctaa ccataaagga ttagtttatc ggcgttaaat cttttctcat
       61 acactatcgg gttagggaaa atatctggtg ttggggacag agtcagcagc gtattagttg
      121 aatgcttaat atcaggctgc tgaattgtca gcactgtgtt tgcgaagagg atgcgaaata
      181 aatgaacccg gttgatcgtc cactattaga tattggctta acgcgattag aattcttgcg
      241 tatatcagga aaaggccttg ctggtctaac aattgcccct gcgttactgt cacttttggg
      301 ttgcaaacag gaagatattg atagcggcac agtagggttg ataaatacac ccaaaggggt
      361 gctggtgacc cagcgagcgc gatgcacggg ttgccaccgc tgtgaaatct cttgtaccaa
      421 cttcaacgat ggctcagtag ggacattctt ctcccgtatc aaaatccatc gcaattattt
      481 ctttggcgac aacggggttg gctctggcgg cggcctgtat ggcgatctca actataccgc
      541 ggacacctgc cgtcaatgca aagagccgca atgcatgaac gtctgcccga ttggtgcgat
      601 tacctggcag cagaaagaag gctgtattac cgtcgatcat aaacgctgta ttggctgtag
      661 cgcctgtact acggcatgcc cgtggatgat ggccaccgta aataccgaaa gtaaaaaatc
      721 ctcgaaatgt gtgttatgcg gtgaatgcgc aaacgcctgc ccaacagggg cgttaaaaat
      781 tatcgagtgg aaagatatta ctgtgtgaat cttgcaaagg aaaaaatcat ggctaacggt
      841 tggacaggta atatattaag agtcaatctc acgacaggaa atattaccct cgaagattcc
      901 agtaagttta aaagttttgt cggtggcatg ggcttcggct acaaaattat gtatgacgaa
      961 gtaccgccag gcacgaaacc tttcgatgaa gcgaataaat tagtctttgc taccggccca
     1021 ttaactggat ctggtgcccc ctgtagttct cgcgtaaata tcacctcact ttctactttt
     1081 accaaaggaa atttagttgt cgatgcccat atgggtggct tttttgcagc gcaaatgaaa
     1141 ttcgctggat acgacgtcat tattatcgaa gggaaggcga aatcaccggt atggctgaag
     1201 attaaagatg acaaagttag cctggaaaaa gccgatttct tatggggaaa agggacgcgc
     1261 gcaacgacgg aagaaatttg tcgattgacc agtccggaaa cctgtgtggc ggctattggt
     1321 caggctgggg aaaaccttgt tcctctctct ggcatgttga atagccgtaa ccacagcggc
     1381 ggtgcgggaa ctggcgcaat tatgggttcg aaaaacctga aagcgattgc ggttgaaggg
     1441 acgaaagggg tcaacattgc cgatcgtcag gagatgaagc gtctcaacga ttacatgatg
     1501 actgaactta ttggtgcgaa taacaaccat gtcgtgccaa gtacgccgca atcgtgggca
     1561 gagtattcag atcccaagtc acgttggaca gcacgtaaag ggctgttttg gggcgcggct
     1621 gaaggtggtc cgattgaaac gggggaaatt ccgccaggca atcagaacac ggtcggcttt
     1681 cgtacctata aatccgtttt tgacttaggt ccggcggcag agaaatacac agtaaaaatg
     1741 agtggctgcc actcttgccc gatccgttgt atgacccaaa tgaatattcc tcgggtgaaa
     1801 gagtttggcg tgcccagcac aggtggtaac acttgtgtag ccaactttgt ccataccacc
     1861 atcttcccga acgggccgaa agattttgaa gataaagacg atggtcgtgt gattggtaac
     1921 ctggttggtc tgaatttgtt cgatgactac ggcctatggt gtaactacgg gcagctgcat
     1981 cgcgacttta cttattgtta cagcaaaggg gtgttcaagc gtgttctgcc agctgaagag
     2041 tatgcggaaa ttcactggga tcaactggaa gcgggtgacg ttaatttcat taaagatttt
     2101 tactaccgtc tggcgcatcg tgtgggtgag ctgagtcacc tggctgatgg ttcatatgcc
     2161 atcgcagaac gctggaattt gggtgaagag tactggggct acgcaaaaaa taaactctgg
     2221 tcgccgtttg gctatccggt tcaccatgcc aatgaagcgt cagcgcaggt cggttccatt
     2281 gttaactgta tgttcaaccg tgactgcatg acgcataccc atatcaattt tattggttcg
     2341 ggtttgccat tgaaactgca gcgtgaagtg gcgaaagagc tttttggttc tgaagatgcc
     2401 tacgatgaaa ccaaaaacta cacgccaatc aatgacgcaa aaattaaata tgcaaagtgg
     2461 tcgctgttgc gggtctgttt gcataacgcc gtcaccctgt gcaactgggt ctggccaatg
     2521 accgtttcgc cgctgaaaag ccgtaattac cggggcgatc tggcgcttga agccaaattc
     2581 ttcaaagcga tcaccggcga agagatgact caggaaaaat tagatttagc tgcagagcgt
     2641 atctttacgt tgcatcgtgc ctacacggta aaactgatgc aaaccaaaga tatgcgtaat
     2701 gaacacgatc ttatctgttc ctgggtattc gacaaggatc cgcagatccc ggtctttact
     2761 gaaggtactg acaaaatgga tcgtgacgat atgcatgctt cgctcacgat gttctacaaa
     2821 gaaatgggct gggacccaca gcttggttgt ccgacccgcg aaacattgca gcgtctgggg
     2881 ctggaagata ttgccgccga tctggcagca cacaatctac tgcctgtgta aggaatgggt
     2941 gagatgaacc atcgggatga attgccgtta gcgaaagtca gcgaagttga cgaggcgaag
     3001 cggcaatggt tacaggggat gcgtcacccc gttgatacgg taaccgaacc agaaccggct
     3061 gaaatcctgg cagagttcat tcgccagcat tctgcggctg ggcagctggt ggcgcgcgcc
     3121 gtgtttctct cgccacccta ttcggttgca gaagaggagc tgtcagtttt gctggaaagc
     3181 attaagcaga acggagacta cgcggatatc gcttgtatga cgggaagcca ggatgattac
     3241 tattactcaa cgcaggccat gagtgaaaac tacgcggcga tgtctttgca agttgtggag
     3301 caggacattt gtcgcgccat tgcacacgcc gttcgttttg agtgccagac gtatcctcgt
     3361 ccttacaaag tcgccatgct gatgcaagcg ccatattact tccaggaagc gcagattgaa
     3421 gccgcgatcg ccgcgatgga tgttgcacct gaatatgccg atatacggca ggtggagtca
     3481 tcgacggcgg tgttgtactt gtttagcgag cgatttatga cctatggcaa ggcttatggc
     3541 ttatgcgaat ggttcgaggt agagcagttt cagaacccct gaaatttcag ggagatgtca
     3601 tggatagggt ggacagttgc agccactgca ttaggggata atcagatgtc cttcactcga
     3661 cgcaaatttg ttctggggat gggaacagta atctttttta ctggttctgc gtcttctctg
     3721 ttagcgaaca cgaggcaaga aaaggaagtt cggtatgcca tgattcatga cgagtcaagg
     3781 tgcaatggct gtaatatttg cgcccgcgca tgccgtaaaa ctaaccacgt ccccgctcag
     3841 ggaagccgtt tatcgatagc acacattcca gtcaccgata acgacaacga aacgcagtat
     3901 cacttttttc gccagtcatg tcagcactgc gaagatgctc cgtgcatcga cgtatgcccg
     3961 acaggggcgt cgtggcgtga tgagcagggg atcgtgcggg tagaaaaatc gcagtgcatc
     4021 ggctgcagtt attgcattgg cgcttgtccg taccaggttc gctaccttaa ccctgtcact
     4081 aaagtggcag ataaatgtga tttctgcgct gagtcccgac tggcaaaagg ctttccgccc
     4141 atttgcgtca gcgcctgccc ggaacacgca ttgatctttg gtcgcgaaga tagcccagaa
     4201 attcaggcgt ggttgcagca aaataaatac tatcaatatc agctacctgg tgccgggaaa
     4261 ccgcatctgt atcgccggtt cggtcaacat ttgattaaaa aggaaaatgt atgaacccgt
     4321 cgcaacatgc tgaacagttt cagagccagt tagcgaacta tgtgccacag ttcactcccg
     4381 aattttggcc ggtgtggttg atcattgccg gagtattgct ggttgggatg tggttggtgc
     4441 tggggctgca tgccttgctt cgtgctcgtg gcgtgaagaa atcagtcacc gattatggtg
     4501 agaagattta tctttactgc aaagcggtca gattatggca ctggtcgaat gcgttactct
     4561 ttgtattgtt gctggcaagt gggctgataa atcactttgc gctggtgggc gcaactgcgg
     4621 ttaaaagtct ggttgcggtg catgaagttt gcggattttt gttactcgca tgctggctcg
     4681 gctttgtgct gatcaatgcc gttgggggta atggtcacca ctatcgcatt cgtcgtcagg
     4741 gggggctgga gcgagcggca aaacaaacgc gattttattt gtttggcatt atgcaggggg
     4801 aagaacatcc tttcccggca acaacccagt ctaaatttaa tcccttacag caggtcgcct
     4861 atgttggtgt tatgtatgga ttgctgccgt tgttactatt gacggggctg ctgtgtctct
     4921 atccgcaagc cgtgggagat gtgtttcctg gcgtaagata ctggttattg caggcgcatt
     4981 ttgctctggc atttataagc ctctttttta tcttcggtca tctttatctt tgcaccacgg
     5041 ggcgtacgcc acacgaaacc tttaaaagca tggtcgatgg ctatcaccgg cactaacgaa
     5101 tgatcattac acgagcagac ctgcgagaat ggcgtatcgg agcggtgatg tatcgctggt
     5161 tcctgcgcca tttcccacgc ggcggtagtt atgccgatat tcatcatgca ctgattgagg
     5221 aaggatatac ggactgggcg gagagtctgg ttgagtatgc gtggaaaaaa tggctggcgg
     5281 atgaaaactt cgcgcatcag gaagtttcct ctatgcaaaa actggccaca gaccccggag
     5341 agataccatt ttgcagtcag tttgcccgca gcgacgatca tgctcgcatc ggctgttgtg
     5401 aggataacgc ccgaatcgca accgctgggt atgccgcgca aattgccagt atgggttata
     5461 gcgtgcggat tggaagtgtc ggctttaaca gtcacattgg cagctccggt gaacgcgcac
     5521 gggttgctgt aaccggtaat tcttcgcgga taagcagcgc tggagatagt agtcggattg
     5581 cgaataccgg aatgcgagta cgcgtttgta cattgggtga acggtgtcat gtcgccagta
     5641 atggtgacct ggtgcaaatc gccagctttg gcgcaaacgc cagaattgcc aacagcggcg
     5701 acaatgtaca catcattgcc agcggtgaaa attcaacggt agtgagcacg ggcgtggtcg
     5761 actcaattat tctcgggcca gggggaagcg ccgcactggc atatcatgac ggcgaaagag
     5821 ttcgcttcgc cgtcgccatt gaaggtgaaa acaatattcg aaccggagtc cgctatcgtc
     5881 ttaatgaaca gcatcagttt gttgagtgct gatagcttta cctttaatca aacgaaagac
     5941 gccgacgagc acgggatgca ggctttacga ccgcccgagc catcgcctca ccaatttctg
     6001 cacatgccgt aagtccctga acgaaaggct ggtcgtgcat caaccagggc aacgcgccga
     6061 acgctacgcg tccacgaata tcttcgggtt gctgtaacgt ataatcttcg ccaacatcag
     6121 ggatttcatc ccctgttttc tgtaattgct cgcgtagccc agggaaagga atatctttca
     6181 ctttaagcgg acgctgtccg cgggcatcaa taaaaacgtc aaacgagtag ctgttgtctt
     6241 ccgttttcag gacggtgcgc gactcattaa tttccatttt gtagtcttca ccgagggcga
     6301 gaatatgaat gattcccgct tcacgtaagg caagtaggcg acgaatagac tctgaaggga
     6361 ttgccgcata attatcgatg aaaaccctgg caaggccttt actgaaccgt ttatggtcgt
     6421 gttcattcag atgaggaaca atttcctgta cggcttcatg caggcgcaga atgacataac
     6481 gccagggaac agtatgtttt tctcgtttat tgcgttcgac ttcctggaga tttttttctg
     6541 cccagtcaaa tggggcgcgt tgtttgcgct cggcaaacca ggcttgagca aaggaatcga
     6601 cattcaggct ctctaaggct atgcgttggc tccagtctgg atcagcaaac ttgatttcct
     6661 ctactatcaa tctaaatacc cgatccaaaa ggccctcttc acctttttga atctcagcat
     6721 ttaatgcctg atcggtgacg atgtgtaagg gctcgtaggg aatagggcaa tagaaatcgg
     6781 cttcgggtaa aatacccgtg cgcgacatca acgtaatatt tagcttttca cttgcgttat
     6841 cgcggttaaa aacgacgtgt tgtttatcat cttcaatgaa cgaaccatgc tgaatagcca
     6901 ctgccattgc cgcatccagt ccgctcaagg atgttcccat aatacccacg ttacacgcat
     6961 cgacctttgc ttccatcagg cctgaccacg gactgggaaa atacgttctg gttgcttctt
     7021 cttcatcagg ccagacgtga cccgtggcaa tcaccgctaa atcaaacgtc tcgctgggta
     7081 aatcctgatt tgtagcgagc atgacgccag catttgtaat ttgcagatcg gtaacctggc
     7141 atgattcata aacagccact gcaaattttt gctgtcgtgc ctggtctact aatcgtaaaa
     7201 attgatcgcg gaaatattcg cccagcagaa ttcgcggtaa aaactgacga tcgtgcaagg
     7261 tttctttttt aacgccataa cgctggagat ggctggcttc ttgcttttgt agccattcga
     7321 gatacgtaca attaatcggc ggtatttcaa tactggcaat atttgccagc atcatttttg
     7381 agttttcctc atcactgtat ggcatcccga caccggcctc gtcagcctgc tcgaagatag
     7441 aaatagaaag tggagtttgt tgctgtagaa gcgagaataa ggtgtagatc cccgtaggcc
     7501 cggcacccac aatagcaatt tttttcattg ttacttacct gtacgcattc tttcgaattt
     7561 agtgttatca gataagtttg gttgagattt ttagaatttg agaaatttca gacagggatg
     7621 aaaagggcgc taaccgccct tgattaaatt ctgtcaggcg agttttgcct gattgatttg
     7681 actcagtggc tcattgacat cgaaaacttt ggcgacaact tcctcaacgc cgagattttt
     7741 aagtcgggca gtatgttttt ccagataggc aagggcgctt ttttcatcag taaaaagata
     7801 gatcccaccg gcttcgtggt tcttttcact ttctgtccat accttccaca gaaaaccagg
     7861 ttcctgatta atcgactcag caagcggttt aagctgctca gccattgcgt cgccaaatgg
     7921 gccattgaaa gcaaaatgaa gttgtaacaa agttgccata gcgattctcc ttctgataac
     7981 gcatacgaag acagtgtaaa tcttttaacg tattggatac agagaagaaa cagagggaaa
     8041 aaggagaaga ccagattaag aaaatctggt gcgtccgagt ggactcgaac caccgacccc
     8101 caccatgtca aggtggtgct ctaaccaact gagctacgga cgcaggatgg tgcgttcaat
     8161 tggactcgaa ccaacgaccc ccaccatgtc aaggtggtgc tctaaccaac tgagctatga
     8221 acgcaacgtt gtaggtgaca acggggacga atattagcgg cagagtggga aggtggcaag
     8281 aggcaaaacg taattttctg cgctatttcg accgtttgca gagcttttaa gcaaattggc
     8341 tatattttgt tgatttgcaa gggcgatttt tattcaggat cgcatttaca tctgatacaa
     8401 cccaataatt cgtgtatcag agtttaaaca gagttgtttg ccaccatctc aaatctgtcg
     8461 tggataaatc ctccgtagcc tttacactta ggtagcaata aaggcacaca gatgcagcaa
     8521 aggaataaat catgggatct gatgcgaaaa acttgatgag cgacgggaat gtgcaaattg
     8581 ttaagaccgg cgaggtcatt ggcgcgacgc aacttactga aggcgagttg attgttgaag
     8641 ctggcggaag agccgaaaat accgtggtca cgggggctgg ctggttgaaa gtggcaaccg
     8701 gtgggatcgc caaatgcaca cagtacggta acaatggcac gctatcggtc agcgacggtg
     8761 ccattgccac agatattgtt cagtccgagg gaggcgcaat tagtctctct acgctcgcta
     8821 cggttaatgg ccgccatccc gaaggtgaat tcagcgttga tcagggttat gcctgcggtt
     8881 tgttgctgga aaatggcggt aacctgcgtg tactggaagg acatcgcgcg gaaaaaatca
     8941 ttctcgatca agagggcggc ctgttggtta atgggacaac ctcagcggtc gtggtagatg
     9001 aaggtggtga attgttggtg tatccaggtg gggaagccag caattgtgag attaatcagg
     9061 gcggcgtttt tatgctggcc gggaaagcca gtgatacgtt gcttgctggt ggcaccatga
     9121 ataatctcgg tggtgaagac tctgacacta ttgttgagaa tggatccatt tatcgtctgg
     9181 ggacggatgg ccttcagctc tacagttccg gtaagacgca aaacctgtcc gtgaatgtgg
     9241 gtggtcgggc tgaagtgcat gccggtacgc tggaaaatgc ggtaatacaa ggtggaacag
     9301 tgatcctgtt gtcacccacc agcgcggacg aaaattttgt cgtagaggaa gatcgcgcac
     9361 cggttgaact gaccgggagt gttgcattac tggacggcgc ttcaatgatt attggctatg
     9421 gcgcagatct gcaacaatca acgattactg tacagcaggg cggtgtgttg attctcgacg
     9481 gcagtacggt aaaaggtgac ggtgtcactt ttattgttgg taacatcaat ctgaatggcg
     9541 gaaaactgtg gctgatcact ggtgcggcaa cgcatgtgca actgaaagtg aaacgcctgc
     9601 gcggagaggg agcgatttgc ctgcaaacca gtgcgaaaga aatctcacct gacttcatca
     9661 atgtgaaagg ggaagttacc ggggatatac acgttgagat aacagatgcc agtcggcaaa
     9721 ctctgtgcaa cgctctgaaa ttacagccag acgaagacgg gattggcgca acgctccagc
     9781 ctgcgtaaaa acacaggcgc aacccgtgaa ggttgcgcct tgtctttatt agcgggatgc
     9841 tcgttgcaga atgataactg acggcagacg ttgcaggaac cgcatacgca gcatcatcat
     9901 aatggctgcc gacgtcaggc caataataaa gcctatccag aagcctgctg gccccatagg
     9961 ttcaacgacc agatcggtca gtgccagaat atagccgctt ggcaagccca gcacccagta
    10021 agccgtaaag gtgatataga aaatggaacg cgtatcttta taaccacgca aaatcccact
    10081 gccaatcacc tggattgagt cagaaatctg atataccgcc gccagcaaca tcaaatgcgc
    10141 agccagcgtt acaacctcgg gattgtcgtt gtacaacagg gcgatttgct cccgcagtga
    10201 aaccgtgaat atggccgtca gggttgccat acagacaccc accataagcc cggtccgcgc
    10261 agcggtttgc gcatccagcg ttgagccctg acccagacga taacctacgc ggatagttac
    10321 cgctgccgcc agcgacattg gaagcacaaa catcagtgaa ctaaagttca gggcaatctg
    10381 gtgtcctgcg acatcaacaa taccgagcgg agacactaac agagccacga cggcaaacag
    10441 tgtcacttca aagaatagtg ccagcgcaat cggcaaaccg agttgaatca gtcgtttcat
    10501 aaccgcagga tcgggtttcg cggtgccttt ttcgttacga atatcgcgca tggagcgggc
    10561 gcgtttaatg taagaaacca tggcaaggaa catgacccaa tacaccgccg cagtagccac
    10621 gccacaacca acgccaccga gctcaggcat accgaaatga ccataaataa agatatagtt
    10681 caccggaatg ttcaccagca ggccgataaa gcccattacc atacccggct tggtttttgc
    10741 cagaccttca cattggttac gggcaacctg gaagaacaga tagcccggcg cgccccacaa
    10801 caacgcacgc agataaccca cggctttgtc cgccagagcc ggatcgatgt tttccatgga
    10861 gcagataatg taacctgcat tccacagcac aagcataatg agaacggaaa caaaacctgc
    10921 cagccagaaa ccttgtcgca cctgatgcgc aatgcgctca cgtcgaccgg aaccatttaa
    10981 ttgcgcgata accggcgtta atgccagcag cagtccgtga ccaaagagga tcgccggaag
    11041 ccagatagaa gtaccgatag cgaccgccgc catgtcggtg gcactatagc cgcccgccat
    11101 cacggtatcg acaaaaccca tcgcagtttg ggcgatttgc gcgagaatca ccgggattgc
    11161 taatgctaat aacagacgcg cttcactgat atacttctgc acgtgaacac cttttatttg
    11221 tagttatatg agagactaaa aagccgcctg aatgggcagc aaaaagaaga agcaggggaa
    11281 attcagtcta ttgtagcgag gatttattaa ttctccagtg aaaaaatagt cattcttacc
    11341 ccttcactgg taagggcgtt tgccacctgc tattgtgctg aacagaatgt cttaactgat
    11401 ttcaggagtt gtaagtatgt ttacggggat tgtacagggc accgcaaaac tggtgtcgat
    11461 tgacgagaaa ccaaattttc gtacgcatgt ggtggagtta cccgaccaca tgctggacgg
    11521 cctggaaaca ggtgcttccg tggcgcataa cggttgctgc ctgaccgtga cggaaattaa
    11581 cggcaaccat gtcagttttg acctgatgaa agaaacgtta cgcattacca atcttggcga
    11641 tttaaaagtg ggggattggg taaacgttga gcgtgcggcg aaattcagtg atgaaattgg
    11701 cggacactta atgtcaggtc atattatgac caccgctgaa gtggcgaaaa tattaacctc
    11761 ggaaaataat cgccagatct ggtttaaagt ccaggatagt cagttgatga aatatatcct
    11821 gtacaaagga tttattggca tcgacggtat tagcctgacc gtcggcgaag tcacgccaac
    11881 gcgtttttgc gtccatttaa ttccggaaac tctggaacgc acgactcttg ggaagaaaaa
    11941 actcggcgca cgcgtcaaca ttgaaattga tccacaaact caggcagtgg tagatacggt
    12001 agaacgtgtg ctggcggcac gagaaaatgc catgaatcaa ccaggtacag aagcctgatc
    12061 tgaaatcgcc cccggagagg gggcgataga atagccttta gcgagcgact cgaaggccgt
    12121 tttcaacacc gcgtgagaac acgacctgcc agagctgaat atcacgggcg cggaaagcac
    12181 ctgcacaggc attcagataa taggtaaaca ttcgtttaaa gcgttcactg tagttatccg
    12241 caatttctgg ccatgcggcg aggaatcgtt cataccacgc catcaacgta gtatcgtaat
    12301 cagcaccgaa gttatgccag tcttccatca cgaagtgggg ttcgctggat tgagcaatct
    12361 ggcgtacaga gggcaggcaa ccgttcggaa aaatatattt attaatccag ggatcaacat
    12421 tcagatcggt ttttttcgaa ccgatagtat ggagcaggaa tataccttcc ggtttcaaat
    12481 tacgatccac caccgcaaaa taggtatcgt aatttttcgg tccgacgtgc tcgaacatcc
    12541 ccacagaaac aatacgatca aactggtcgt tcaggtcacg ataatcttgc agcaaaatgg
    12601 tgacatccag gccttcacag cgttcctgag ccattttttg ctgttcggca gaaatggtga
    12661 cgccgaccac gcttacgtca tagttagatg ccatgtagtg cgccagtccg ccccagccgc
    12721 agccaatatc cagtacgcgc atccctggtt ttaactgcaa tttttcacaa atcattttga
    12781 gcttcgcctg ctgggcagat tccagattat cggcatcttt ccagtaagcg caggaatatt
    12841 gcatgaaggg atcaagcatg cggctgaaca agtcattacc caaatcgtaa tgctccttgc
    12901 cgactatcca ggcacgtttt ttactctgca gattgaagag acgagcgctg gcaatacgca
    12961 gcgtgtcttt gaaatgatgg gggagttggt tctcgagacc tgcgcgtaag actttgctaa
    13021 aaaacatatc cagtcggtca cattcccacc agccatccat ataactttcg cctaacccca
    13081 aagagccttc ttgcagaacg cgtttaaaaa aatcggggtt tttcacacga atatccgccg
    13141 gggcagaacc gttaatggct ataccggcac ggctaagtaa ttcgttggcg atacggtacc
    13201 agttgtcatc cggtacactg acttcttcta tacacgatga actcatagtt tctccatcac
    13261 tggtcggtga tcagaacctt aaaacagcgt agacgctttt tgggctttgt gagaaatctc
    13321 gcagaaaaaa ccgcgagtct ataatccgac gtagaacaga gggaaaggag gaacctttgc
    13381 cgaaatgacc atccatagtg agtcgggaac gatcctgtcc ccgtaaaaac ataatattta
    13441 tcgtagcttt aacaactcga aattagtata ggccgctaat ttcaatcata caacaaaatt
    13501 ttgttagcag cctaagtata agtatatcga tatagatcag tgtgattcgc tatgagcgac
    13561 ttcggcattg ccatgattct ggcagccaac ttcttcacaa cgttgcatca tgtaacccag
    13621 agcgaccagc actactgttg ataacatcac gctggtggtg gtgagcaatg gcgtgctgat
    13681 actgatcagc caggaaacta ccagacttgc gaggaagcac agacccagtt gtagagtgtt
    13741 ctgcaacgct gcggcgcgac cagttgcgtg tgggaaggga cgcagcgcct gggcgacaac
    13801 aatcgggtag atcgcgccat tggcaatcgc catcacacag aatgggatca ggatttcgac
    13861 cagcgacaca tggctaatga agcctgcagc ccaggtcgca atgacgctga ctgcaaacag
    13921 caccagcaac cacggtaata actgcttgcc ttgccatttc tgcagcgcgg cgcgacagcc
    13981 ataaccacca atcagaaacg cgatagtttg cgggacataa cttaaaccaa taactgccgg
    14041 gctgtagccc atttcactaa ggatgaacgg tgaaccggtc agccatgcaa aaaaactggc
    14101 tgaacaggcc gcgtatatca gcacgttgcc gcgataggtt ttagaacgta gcaggtcggt
    14161 aaaggtcaga ccatcctgac tattgttacg ggccttcgtc gtgggtttga gccagaaaat
    14221 aggcagaatc agcaccacgg taatggcaaa cagggtggcg aaaatcgcct gccaggaaaa
    14281 atggaccagc agccagcttc ctaacagagg agccagggcc ggagatagac ccaccagcgg
    14341 catgatggtc gcaaaaattc ggttaacttt ctgtgaagga taataatccg tcactaacgc
    14401 ttgccagata accgccgcgg cgcagacacc cacagcctgt acaaaacgca ataccagcag
    14461 cgtagcggcg ttttctaccc acagcatccc cagactacct aacgcaaaaa ttgtcaggcc
    14521 gattaataac accggtttac gaccataacg gtcggagagt ggcccccaca gaagctgggc
    14581 tgcggcaaaa ccggcaagga acagactaag gctggcactg acagcagacg caggcgtttg
    14641 caggtcggcc tgtatggcgg cgaaagcagg cagatacata tcggttgcca gaaaaccgag
    14701 tacgctcaaa cccgccagcc agactaaaaa tcttttccca ggttgcatgt atattctcta
    14761 aatctctcaa ttaaacaccg ccgcaaagtg tagggagtga aattagtgtt gtgaaacggt
    14821 aatatttgat gactggtttc aaaaattttg caggcagact atgtggtcag aatattcact
    14881 cgaagttgtt gatgcggtag cgcgtaatgg tagttttagc gctgcggcac aggagctgca
    14941 tcgcgttcct tctgcggtca gctataccgt gcgtcagctg gaagagtggc tggcggtgcc
    15001 gctctttgaa cgacggcacc gtgatgtgga gctgaccgct gctggagcgt ggtttctcaa
    15061 agaagggcgc tctgttgtca aaaaaatgca gatcacccgc cagcaatgtc agcagatagc
    15121 gaacggctgg cgcggtcagt tagctatcgc agtggataat attgtcaggc cagaacgtac
    15181 acggcagatg atcgttgatt tttatcgcca ttttgatgat gtcgaacttc ttgtctttca
    15241 ggaagtgttc aacggtgtct gggatgcgct ttccgacggg cgcgtggaac tggcgattgg
    15301 cgcaacacgc gcgattccgg taggcggtcg ttatgccttc cgggatatgg ggatgctaag
    15361 ctggagttgc gttgttgcca gccaccaccc gctggcgttg atggatggcc cgttcagcga
    15421 tgatacgttg cgcaactggc cgtcgttggt gcgcgaagac acctcgcgaa cgctacctaa
    15481 acgtattacc tggttgctgg ataaccaaaa aagagttgtc gtgcctgact gggaatcatc
    15541 ggcaacctgt atctcggcag gattatgcat agggatggtg ccaacacatt tcgccaaacc
    15601 gtggcttaat gaggggaagt gggtggcatt agaactggaa aacccctttc cggattcggc
    15661 atgttgtctg acatggcagc aaaatgatat gtcgcctgcg ttaacctggc tgcttgagta
    15721 tctcggcgat agtgagacgc tgaataagga gtggctgcgg gagccggaag agactcccgc
    15781 aacgggtgat taacgacgat agtcgcggaa cgggccgtca gccacggagc ggcgttcaat
    15841 caagcgcgga tgcacttcaa tggactgcgg ttcttcacgt ttgttgacga tacgatccaa
    15901 cagcatgttg aacgctgttt cacccagcga atcttttggc tggtggatcg tggtcagcgc
    15961 cggcgtaaaa tagcgggcgt tgcgcacgtt atcataaccg atcagcgaaa catcctgcgg
    16021 gacgcgcaga cccatttcat cagcagcaca aagtgcgccc attgccatga tatcgccacc
    16081 acagaagacg gcagtagggc gatgcggctg cgacagtatt tgctgcatgg cgcgataacc
    16141 agattcgggt tcaaagtcac cctgaacaat ccagctttcc ggcaccttga tcatcgcttc
    16201 ttccatcgcc ttcataaaac cggcaaggcg gcctgcgccg gtgttacgtt ccagcgggcc
    16261 ggggatgacg ccgatttcgc ggtgaccgcg ttcaatcaga taacgcccgg ccatgtagcc
    16321 gccttcgaac gcgttatcaa tgaccgcatc ggtgaagtca gcttttgctt caccccagtc
    16381 catcaccacc attgggatat ggcgatactc ttccagcatc gccagcaacg gctctgggta
    16441 ctcagaacac atcaccagca gaccatcgac gcgtttttgc gccatcatcg acagataagc
    16501 ccgctgtttc tcaagattgt tccacgcatt acccagaatc agggtgtaac ctttctggaa
    16561 gcaatttttt tcaaccgctt caatgatctc ggcaaaatag gccgcttcgc tgctggtcgc
    16621 cagcaaaccg atagacttgg tgtggttaac cttcaggcta cgcgccaccg cgctagggga
    16681 gtagtgtaat tctttaatcg ctgcccacac ggcgttgcgc gtttcttcag cgacgaaacg
    16741 tgttttgttg atcacgtgtg acacagttgt agtggaaacg tttgctcgtt tcgctacatc
    16801 ttttattgtt gccattccat ttcactccag accctaactt cagctcctgc aaaatcgcaa
    16861 ggtaaacgtt tgcctttaca caccttacgc acaacaaaag tgttgcggta cgccggaaaa
    16921 acgacacgga gcgtcaggaa ggggtcaatg gccagtacgc aaatagtgta agcgtggaat
    16981 tttggccgat cttgacgaaa aggggaagtg gtaaaaacac ttatcattat aaatgacggg
    17041 agattttttc atcacagtgt gtaaaaatgc gtaatatcac tttttgtgtg gtaataaaaa
    17101 aggagaaaac ttgatgagca ccgaccttaa attttcactg gtaacaacga ttatcgtcct
    17161 cggtttgatc gtagccgtgg gtttgactgc cgcgctgcac tgatttcctc tcgagggagc
    17221 gcgttctccc tcgcatctcc ttcctgtttg ttagcaaaat ggcaaaagtg ttttctcttt
    17281 ttgttatttt catttttttg tgattgctgt catgctttcg ccactttaat gaatatcgcg
    17341 taatgacgcg aagaggtgga gataaggcat gaaaattaac tatccgttgc tggcgctggc
    17401 gattggcgcg tttggtatcg ggacaacgga gttctcgcca atgggcttgt tgcccgtcat
    17461 tgcgcgcggt gtggatgtct cgattcccgc tgccggaatg ttaatcagtg cctatgcagt
    17521 tggcgtaatg gtgggcgcgc cgctgatgac gcttctactt tctcatcgtg cccgccgcag
    17581 tgcgttgatt ttcctgatgg caattttcac gctaggcaac gttctttccg ccatcgcgcc
    17641 ggattatatg accctgatgc tttcacgcat tttgaccagc ctgaatcacg gagcattttt
    17701 tggtttgggt tcagtcgtgg ccgcaagcgt ggtgccaaaa cataaacagg ccagcgcagt
    17761 tgccactatg tttatggggt taaccctggc aaatatcggt ggcgtgccgg cggcgacctg
    17821 gttgggtgaa accatcggct ggcggatgtc atttctggca acggcggggc tgggagtgat
    17881 ttcaatggta agtctgttct tctcattacc taaaggtggt gcaggggcac gacctgaagt
    17941 gaaaaaagag ctggcggtat taatgcgtcc gcaggtgctg tctgcattgc tgacgacggt
    18001 actgggagct ggtgcaatgt ttactctcta cacctatatc tctccggtac tgcaaagtat
    18061 tacccacgca acaccggtgt tcgtcacggc aatgctggtg ctgattggtg tcggattctc
    18121 tatcggtaac tatctcggcg gcaaactggc agatcgttca gttaacggca cgttgaaagg
    18181 ctttttgttg ctgctgatgg tgattatgct ggcaatcccg ttcctggccc gcaatgagtt
    18241 cggcgcagct attagcatgg tggtgtgggg cgcagcaacc tttgcggtcg taccgccgtt
    18301 acagatgcgc gtgatgcgtg tcgccagtga agcgccgggt ctgtcttcat cagtcaatat
    18361 tggtgccttt aatcttggaa atgcgctggg agcagctgct ggtggtgcgg taatttccgc
    18421 tgggctggga tacagctttg tgccggtgat gggagcgatt gtcgcgggac tggcattatt
    18481 gctggtgttt atgtcagcca gaaaacaacc tgaaacagtt tgcgttgcta acagctaatt
    18541 aaacaaaaac gcagaaggat aaccttctgc gttacagttg ctgacaacgt gcgatttatc
    18601 ttgccggatg cggtgttaac gactcatccg cgttgacatg aaaagacgcg ttaggcgtcg
    18661 tatcatgcag cgcttgtcgg atgcggcgag tgccttatcc gacctacatc gatgatccgc
    18721 gtaggcctga taagcgtagc gcttcaggca atgctgcatt tgccatcagt tattatgcag
    18781 cgagattttt cgctacgaat tcccagttca ccagcgccca gaagtgctcc agatagccag
    18841 gacgtgcatt gcgatagtcg atgtaataag cgtgttccca gacatcaacg gtcagcagcg
    18901 gagtcgcatc ggtggtcagc ggagtacccg cgttagaggt tgaaacgata gccagtttgc
    18961 catcgctgtt tttcaccagc caggtccagc cagaaccaaa gtttttgatc gctgcatcag
    19021 taaactgcgc tttgaaatcg gcaaagctgc caaaagatgc ggcgatagct tcagcgactt
    19081 ttccagtcgg ttcgccaccg gcgttcggtg ccaggcagtt ccagtagaaa gtatggttcc
    19141 agacctgagc tgcgttgttg aatacgccac cttcagagct gcgaataatc tcttccagtg
    19201 atttaccttc aaacgcggta cctttaatca ggttgttcag gttagtgaca taagtctgat
    19261 ggtgcttgcc gtagtgatac tcgatggttt ccgcagaaat gtgcggtgcc agagcatctt
    19321 tagcatatgg tagtgcaggt aattcgaatg acattgctac tctcctttat tattaatttg
    19381 catacgtaca atagccttat tgtgcgtatg gatagggtag caaattaaaa ggtgagacaa
    19441 aagatgaact taccctgttg ccgtaacaac agggtaaagt tttagcgaag tgtttttggg
    19501 gtcattaccc gacgagcgcc aacatagtgg cgctgccagt agtcttcact gagagaagtg
    19561 atttgaattt cctgacctgt gcgcggtgac tgaataaatt tgccgttgcc gacatacacg
    19621 ccgacatgat cggctgtgcc gcgtccctga gtacggaaaa agaccaggtc gccgtttttc
    19681 agttcactac gttcgattgg cgctgcatca cgcaggtgat acatttcatt cgccgtacgc
    19741 ggaatacgaa ttttcaccaa atctttataa gcgtaataaa ccaggccgct gcaatcaaaa
    19801 ccggtacgcg gtgagctgcc accccaacga tatggcttac caatttgctg catcagttta
    19861 ttcattgcca ctttcgtagc tttttgcacc ttcgctttgt gcgcgtcggc aagagttcct
    19921 gacgcggcat ttttgacttt cacacaatgc gatttatagc ccttacgttt ggtacacttt
    19981 tcggtaaagc tggctgaggc cgttttattg acggcgtgtt tgctgcgagt tttggcgttt
    20041 ttagaagcgg taatggaaga ttttttaacg gaagaagcgg ttttgctttt ggtcgtactt
    20101 ttactggcgg cttttttcgc tgttttctgg gtctttttgg tggttgccgt gcttttcttt
    20161 ttatctgctt ttttggtgat atgagtggta gcagaactct ccctggcttg ctttgaagca
    20221 tgggccatag gcgttaaagg cagggtggta aaaagtaaag cacagagcgt gatcgaaata
    20281 cggtttatcc gcgccactga gcgtccctct gttaaggcag gtctatcatg cctgcgtctg
    20341 atttacaaaa cttgatgatt ctaatgcata gaagaagaga aagttaatag cttttattgt
    20401 taaaaaatac gtttcgttgg cgagcgcaaa aaaatgcagc agtgaaagct aaaaatggtt
    20461 ataaatacgc cagtttgccg gcatcagaga agaaacgaat atttgaaatg caacaaatag
    20521 agcaaaggga taaaatagca aaagcgtgat gaaaatgtta ttttccgatg ccgcactgac
    20581 ccgctacaat gtcagattac tgccgtaaag aagtttgagg aagcaagaca atgagcacca
    20641 ctatcgaaaa aatccaacgc cagattgctg aaaacccgat cctgctgtac atgaaaggtt
    20701 caccgaaact gccgagctgc ggtttctctg cccaggcagt ccaggcgctt gccgcatgtg
    20761 gcgaacgttt tgcctatgtt gatattctgc agaatccgga cattcgtgcg gaactgccga
    20821 aatatgctaa ctggccgacc ttcccgcaac tgtgggttga cggcgagctg gtcggcggtt
    20881 gtgatatcgt gatcgaaatg tatcagcgtg gcgaactgca gcagctgatc aaagaaactg
    20941 ccgctaaata caagtctgaa gagccggacg cggaataatc cgtcctgcaa tactgacgga
    21001 tgcgaacggg catccgtcag tcctttctct atccccaatc cagcccttgt ggcgaacttg
    21061 aaaatcccgc ctcacgcagc agcgtaaaca tcggcgtttg ccggaccggt agatcattca
    21121 cttctgttag cgtaaagcgc agccgtggtt cgcgacgcag tgcggtagtc agcgcgtgga
    21181 aaacctctgg ggcgagtaat tcctcttttt cctgccagac cagcattttt ttgccacctt
    21241 gcgccagata gagtaacaat ttgccgccag aaacgaccac cagcgcaccc gcccgacgcg
    21301 ttggaaccag cgttgccgga tgtgcaggcc agggcagaag atttccccac acattagccg
    21361 gatcgttggc agagagcgcc actggtgtat agtggcgcgt ttgcgtggct tgtgtcgcca
    21421 gatcgcgcaa tcggtcaata gtcagacgtt cagcgaattg cgcgccaccc agaccttcta
    21481 caaaacgacc tcgcataatt cgcccggtgt cttccatact tcgacaaagc gtttgcatcg
    21541 atggaaaccc gccagggata ttttcggcta tcactgcctg acgactgatg atgccgtagc
    21601 ggtcgagcat attttccgcc agcgccagca tcctttcggt atcgtttagt ggctccacct
    21661 gcaataacga ccagcgtcca gccagatttg gtgtgttgta agatacccgc ggcgagacgg
    21721 gttgcgcata gacaggacgt ccacgacgag cccggtgact gcggcgagtt gaggtgcgtg
    21781 cgttggaact gctgcgggtg agggcgcgta acggtgccca aatgtcgctg gtgatgacgc
    21841 cttgccagac cagcgcccat aacgcctctt gcagggcaga gagatcaacc gattcgccga
    21901 ttttgtcgcg tatccgttgg ctgatttgtt gtgcaaacca ggctcctccg tcagccagaa
    21961 cagcgactat cgcttgctgc agcgccgaac gattcgcctg atccgcttcg gcgggagtga
    22021 acgattctgc agcatattcc tgtagatgca gtgccaccag gccgtcatct tcacccagtt
    22081 ttttttgccc cgaccagata accgcaccgg ttgccagtaa ttcatcgagc atttctgacg
    22141 aatagtcgcg tacgcgggca ggcaggatct ggctttccca gagtgaggcg ggtaaaccga
    22201 ctccggcaag ctgttcgatc acccgcatca cgccatctac gccctcataa acgcctggcg
    22261 atgttgaggc aaagagcgcc gggctaccat cggtggcggg taatacgccc tgacgttcca
    22321 gcagcaatcg cgcataggtc gtggctgcaa cgggacgcgt cgcttctctg gcggcttgca
    22381 gcgagcgcaa acgcagacga cgaaatactt catcgctcac ccagatgtct tgttgcagat
    22441 tcatcaccag accctgttca cgcagttgct gaagctgctc ttcgacaatg gcaataccga
    22501 gactaaactc atgagccagt tgttcagccg tgaccagagc atgagcccgg agatagcgca
    22561 gaaagaggtc gcgaagcggg taactgactc tatgtaaata aatctctggc aatgactctg
    22621 gtagtcgtac gccgagggca tcacgcagcc tggcggcatc atccatacag gcaagacgct
    22681 cctgtccgct aatcatcgct gggaagattc gttttactgc aagaagattt tccagatacg
    22741 acgcaacctc ttcactgctg cctgtatgcc gttgcgccag gtcttcaacg gtcattggcc
    22801 ccagttcgcg cagcaggtcg aacaatcctt cttcaccttt cgctcttctg ccaggagcca
    22861 gtcgttgcaa ctcttcttcc acctggcgaa tgacctgcgg gtcgagtaat tcccccggat
    22921 cgacctgtcc gagtagattg cgcagtaact cgctgtccag cgacagtacg gatgcccggc
    22981 gctctgccag cggggcgtcg ctctggtaca taaattccgc gacatagccg aacaataaac
    23041 tggtggcgaa aggcgagggc gtagtagtcg ttacatcgga tatttgaatt tcgccaccgt
    23101 tcaggcgacg catcaaacgt tccagtgcgg gaagatcata aacatcttgc agacattcgc
    23161 gtagggtttc gagaatgacc ggaaaatccg gatatccctg agcgatttcc agcaactgac
    23221 tggcgcgcag ccgttgttgc caaagcgggg tgcgatggcc cggcgtgcgg cccggcatta
    23281 ataatgcccg cgcggcgcat tcgcgaaaac gggcggcgaa aagtgccgaa ctgcctaccg
    23341 cctcgcggac aatttgcagc aacttttctg gttcaaacaa aaaaatcgcg gcatcgggca
    23401 atttaccatc ggtgtcagga atacgtgcaa caatgccgtc atcactggcg accaccgacg
    23461 cgtcagcgcc ccatagggca tgtattcgcc cggcaatcgc cagcgcccag ggttcatgca
    23521 cccgtcttcc atagggagag tgcaaaataa tacgccagtc accaatttca tcacggcacc
    23581 gttcgaggac cagatggcga ctccccggaa cgatgcccgt cgcgttgcgc tgctcctcaa
    23641 tcaacccctg aatattggcg atcgtatttt cttctgccag ccacggggga atagtcccgg
    23701 aaaagaacgc gccatccgcc agcaaatgaa gaaaatcgcc gatcatctcg cctaattcag
    23761 ccggacgtcc gttaccttca ccacgccaga aggggagccg ggcagaacga cccggagcag
    23821 gagtcacaat cacctgatcg cgggtgattt gctggatccg ccatgaggta gcgccgagag
    23881 tgataatgtc gttcacccgc gactcatata ccatctcctc atccagttca cccacccgcc
    23941 gcgaaccggc cttttcttca ccttcgggta ataacacgct atacatgcca cgatccggaa
    24001 tggtgccgcc gctggtaacc gccaacaatt gagcgccagg tcgggcggtc aatatcccgg
    24061 tctccctgtt ccagaccagt ttggggcgaa aagcagaaaa atcgccagag ggatagcgcc
    24121 cggaaagcat atccagcgtg gcgtcaaaaa cacgtcttgg cagatctttc cacggtgcgg
    24181 cacggcgtac gcgggagtac cattcgtcta cctgtaatgc atccatcgcc gcggcggcaa
    24241 cggtttgctg cgcaaggacg tcgagaggat tatgcggtgg tgtcaggttt tccagcctgc
    24301 ctgcgaacat acactctaca atgactgcgg aatcgactaa atcacgccgg gtacggggga
    24361 aaaacagccc tttagataca ccgccaacct gatgtccggc gcgaccaatg cgttgtaacc
    24421 cactggcaac agaaagcggc gttgccacct gaatcaccag atccaccgcg cccatatcaa
    24481 tccccagttc aagactggag gttgcgacca cgcagcgtaa ttcacccgat ttcagcgcct
    24541 gttcggtgat tgctcgttgt tctttagaga cggagccgtg gtgtgagcgg gcaataaaaa
    24601 cgtcgctact ttgtacacgg ttagaggttg cgccggaggt cgactcgaaa tgggccgcat
    24661 caacggcgat agacggggaa cgctgtaagc gtgcggcgta aagctcattt aatcgtgccg
    24721 tcagtttttc cgccagcccc cgcgaattag taaagacaat ggtcgagcga tggcgcaaca
    24781 cttcatcaag gatgcccgtt tcaatatatg gccagatgga gccttcccgg ccggcatggc
    24841 tgtcttcgcc agtgccgctg gcgaccgatg agacatcatc catattggcg accggtacga
    24901 caatccgtat ctgcggatga cgcattgcgg gcgggttgac taccgtaacc gggcgatcgc
    24961 caccaagaaa tgctgccaca tcgctggctg agcgcacagt ggcagaaagg ccaattcgct
    25021 gtgctgaggt gtggagcagc gcatcgagcc gctccagact taacgccaga tgcgcaccac
    25081 gtttactgcc cgctaccgcg tggacttcat caataattac cgtttcgacg ccgcgtagcg
    25141 tttcgcgcgc gcgggaggtc agcatcagat agagtgattc gggtgtggtg atcagaatat
    25201 ccggcggatt acgggtgagt ttgctgcgtt cctgtgcagg cgtatcgcca gtacggatcc
    25261 ctacgcgaag attgacttcc gtttcgccgc gccgccgccg ttcatcggca atacccttca
    25321 acgggatctg caagttgcgc tgaacgtcgg tgcccagggc ttttatcggt gaaatataga
    25381 ggatgcgtga ggttttacgc ttatgcgcct cgcgggtatc ttcgccgcct tcgcggaaga
    25441 gccgatcgag ggcgtagaga aatgctgcca gcgttttccc ggagccagtc ggtgcaatca
    25501 ccagcgcatg ttcgcctcgc gccgccacat gccaggtttg cgactggaca gcggtcggct
    25561 gtttaaaggc gcgaagaaac cagtcgcggg tcgccggtga aaacacgtcc ggcaggagcg
    25621 atgaagggtc tggattatct gccattaacg ctattccccg tgaatatcat gcaggatgcg
    25681 tggaatagcc tcactataga tgcctgaatc attatatgtc atcaggcatc gactcgatta
    25741 cacctcttcg gcggtgggta gcggccagcc tcccagacgt ttccagcggt tgacgatttc
    25801 acaaaacagc acagcagtgc gttcggtgtc gtacagcgcg gagtgcgcct gggtgctgtc
    25861 gaagtccatg ccagcggtct ggcaagcctt tgacaatacg gtttgtccga gtgccagccc
    25921 ggccagtgca gcagtgtcaa aagtggcgaa agggtggaac gggttacgtt tcagcgaggc
    25981 gcgttctgcg gcggccatca taaagctgtg atcaaaattg gcgttgtgcg ccaccataat
    26041 ggcgcggtta cagccgctcg ctttaatacc tttacgtaca actttaaaaa tttcgtgcag
    26101 cgcctcgtat tcgctgaccg cgccgcgatc gggatcgttc gggtcaatgc cgttgaaggc
    26161 gagggcttct ggttgcaaat ttgcgccgac aaatggttcg acgtggaaat gtaatgtggt
    26221 gtccggcatc agccagcctt gttcatccat tttcagggtg atggcggcaa tctcaagcag
    26281 cgcatcggtt ttggcgttaa atccggctgt ttcaacatcg atcacaacag gataaaaacc
    26341 acgaaaacgg tcgcacagac cggtaagttg agcgttatcg gacatctgat tctcttaata
    26401 cggataaatt gcagcgcgca ttatgacaaa tattacttgt cgatgcagta aagatgcggg
    26461 cgcgatgagt tcacgcccgg caggagatta gttgcccaga ccgcgaccgg cgtctttttc
    26521 ttcgattaac tcaattttgt aaccgtccgg atcttccaca aacgcgataa ccgtagtacc
    26581 gccttttacc ggacccgctt cacgggtcac gttacccccg ttttgacgga ttttttcgca
    26641 cgcttcagcg gcgttatcta cgctaagcgc gatgtgacca taagcagtgc cgagttcgta
    26701 tttatccacg ccccagttgt aggtcagttc aatcaccgct tcttcggttt ccgggccgta
    26761 gccaacaaac gccagtgagt atttgtattc cgggttttcg ctggtacgca gcagtttcat
    26821 gcccagcact ttggtataaa aatcgatgga gcgttgcaaa tcgccaacgc gcagcatggt
    26881 atgaagaaga cgcatctttt tatcctcaaa atggctaaca gattcaaaat gttgttttag
    26941 tatagcggcg gctttacgcc gctcgcaatg ttggattaca acgtcgggta atcggtatag
    27001 ccttccgcgc cgccaccgta gaaactttcg gcacgctgtg ggttaagctc agctttgcgc
    27061 tgcaagcggg cgaccagatc cgggttcgca atccagtcac gaccaaatgc caccgcatca
    27121 attaaccctt tgccgatcag cgtttcagct ttttctactg tgtatgcacc tgcgccgata
    27181 atcggaccgt ggaaacgggc gcgtactttt tcgcggaacg catcagtata cggttcaccc
    27241 cccgcccaat ctggttctga catatgcaga taagcaatgc cgcgtttacc cagttgttca
    27301 atcagataca gtgcatcggc ttcttcattc gggccgttat ccgtgttctg gaaagtaccg
    27361 attggcgaaa tgcgaatgcc aatgcgatcg gcaccccatt cttcaatccc ggcatcgacc
    27421 acttccagta ccagacgtgc gcgattttcc acgctgccgc cgtactgatc ggtacgatgg
    27481 tttgaagaag gagaaaggaa ctgatgcagc aaataaccgt gagcagagtg gagctctacc
    27541 agatcaaaac cggcttcacg cgcgttagca atggcctgac ggaaatcatt gacgatacct
    27601 ggaatctctt ccagttcaag cgcacgcggc atggatgttt caacacggat cgcctgacca
    27661 ttttcatcgc gcagagaagt acgtgttccc gcgctaagtg ctgaaggcgc taccggtgcc
    27721 tgaccgccag gttgcaggct ggcgtgagaa atgcgtccgg tgtgccacag ctgcacggcc
    27781 atatgaccat tttcagcatg aacgccagcg gtgatttttt tccatgcggc aatttgctcc
    27841 ggactatgga tgccaggcgc acctgcatat ccttttgcct gggcagaaat ttgcgtggct
    27901 tcactaataa tcaaaccggc actggcacgt tggcgatagt attccgccat caacggggta
    27961 ggaatgtcac ccggttcaat actgcgcaga cgcgtcagcg gtgccataaa aatacggttt
    28021 gccgccgtga tcgcgcccac tttcagtggg gaatacagtt tttcagatga cataaaggct
    28081 cccggtcgtc tggtgataaa aaatgcctgc taaacggcag gcgtcgcaat aatgtttttt
    28141 acatgggcca gcgcgttttc cagtggctcg aaactgcgcg aaattttggc ctgcagattc
    28201 gcgccaagcc acagtgcgta aagcacttgt gcctgttgca gcggttcgcc acaaaaggtt
    28261 aaacaatggt tctcacggcc attttccagc gcctgagaga gcagggcgat cacgccgcga
    28321 gcacctttat ccatcgcgct gcgcatatct tctgacagat cgcacacttc ggcagagagt
    28381 tttactgtca ggcaaccact gatggttcca tgttggcaaa actggttcag tgtttgctgg
    28441 taataagcca gtatgcggtc gcggtagtta ccttcgccgg attgcagcaa ctcagtcagt
    28501 cgctggtgat atgcggcgta atgacgctca agcatggcaa cgccaaacgc ttctttagag
    28561 cgaaagtagt gatagaagga ccctttcggc acttcagcgg tttttagtaa ttcgcttagc
    28621 cccatcccgg tgaatccacg ttgcaggcaa agttgctcgc ccgtcgccag gagatgttcg
    28681 cgagtatcat gttcggtgtg tttgttcatg cgttggagtg tagtagacca gtcggtctag
    28741 tgcaagcaaa gttgctactt tggacattaa cagcttcaat aatggtaata atcttatctg
    28801 aggagggcga agtggcggag caattagagt tctttcccgt ccagagcccg tgccggggaa
    28861 tttgccagtc tgacgaacgc gggttttgtc gcggctgttt tcgtagtcgt gatgagcgtt
    28921 ttaactggaa taaaatgagc gatggcgaaa aacaagaggt gctgcgcctt tgtcggcagc
    28981 gattaatgcg caaattacgc gcaaataagc cagcgtcatc agacgaaccg gagcaaccat
    29041 cactcttttg aacctaatct tgcgtatact cctctcatat ctgataagag gaagcgatta
    29101 tggttcagcg tattactatt gcgccgcaag gcccggagtt ttcccgtttt gtgatgggct
    29161 actggcgatt gatggactgg aatatgtccg cccgccagct ggtcagtttt attgaagagc
    29221 atctggatct cggcgtgacc accgtggacc atgctgatat ttatggtggc tatcagtgcg
    29281 aagcggcgtt tggcgaggca ctgaaactgg cacctcacct gcgtgaacgg atggaaatcg
    29341 tcagtaaatg cggtatcgcg acgaccgcgc gtgaagaaaa cgtcattggt cattacatca
    29401 ctgaccgcga tcacatcatt aagagcaccg aacagtcgct aattaatctc gcgaccgatc
    29461 atctggatct gctgttaatc caccgaccag acccgttaat ggatgccgat gaagtggcgg
    29521 acgcgttcaa acatctgcat cagagcggca aagtgcgtca ttttggcgta tcgaacttta
    29581 cgcctgcgca atttgccctg ttgcaatcac gtctgccgtt tacccttgcc actaatcagg
    29641 tggaaatatc cccggtgcat cagccgttac tgctggatgg cacgctcgac caactacaac
    29701 aactgcgtgt tcgtccgatg gcgtggtcct gccttggtgg tggtcgtctg tttaatgatg
    29761 attatttcca gccgctgcgt gatgaactgg ctgtggtggc agaggagtta aacgcgggct
    29821 cgattgaaca ggtggtttac gcctgggtat tacgtttacc atcgcagccg ctgccaatta
    29881 tcggttcagg taaaattgag cgcgtacggg cagctgtcga agcagaaaca ctgaaaatga
    29941 cccgtcaaca atggtttcgt atccgtaaag cggcactggg gtacgacgta ccgtaagcac
    30001 ttttaggaat agccgccgtt caaaaatgtg tcactggttt acacttattc aggaatgcac
    30061 aatgaacgga ggtcctatga aacgttttag tctggctatt ctggcgctgg ttgttgcaac
    30121 cggcgcacaa gctgccagtg aaaaagtcga gatgaacctc gtcacgtcgc aaggggtagg
    30181 gcagtcaatt ggtagcgtca ccattactga aaccgataaa ggtctggagt tttcgcccga
    30241 tctgaaagca ttaccccccg gtgaacatgg cttccatatt catgccaaag gaagctgcca
    30301 gccagccacc aaagatggca aagccagcgc cgcggaatcc gcaggcgggc atcttgatcc
    30361 acaaaatacc ggtaaacatg aagggccaga aggtgctggg catttaggcg atctgcctgc
    30421 actggtcgtc aataatgacg gcaaagctac cgatgccgtc atcgcgcctc gtctgaaatc
    30481 actggatgaa atcaaagaca aagcgctgat ggtccacgtt ggcggcgata atatgtccga
    30541 tcaacctaaa ccgctgggcg gtggcggtga acgctatgcc tgtggtgtaa ttaagtaatt
    30601 aagaggccag cgtaccttgc ggtggtgctt gctcaagttg cgaaagcgag cagtacagcc
    30661 gccagacaat tgccgccagc tcacgggcgg caggttgatg atgacgggca aggctgtcgc
    30721 aaatccgctg taattcttca agtgtggccg ccagtgattt ttgctgaacg ccacgctcac
    30781 tcatcactcc ccgcaacagt gaaatacagt tatcccgtac tcgcgataac ggatcggaac
    30841 gcgattccca gtcgcgcaat tgccagacaa catgagaaca gttcagcagc actacacccc
    30901 agcgtaataa ccaacggcga gccagcgcat cctggctgtt actcaactga ctgacgtgat
    30961 gataagtgag cgattcaaat tcgctttcac tcagtgttgg atggcggctt agctgatcga
    31021 caaaatcccg gcgcagcgcg cgaatatggc ggcggctttt acgagcatcc gatcctggac
    31081 gcagaatggc gaacgctaac cacgccaacg cgacgccaac gatttttgcc agattatcgt
    31141 taagaaaatc agcaaaatca tacaccgggg gattagtgat agcgataaaa gaacccataa
    31201 aaacaatcag ttgcccccac aatgcggcaa attttggcat ctgcaattta agaagctgca
    31261 ttgtcgccag cagtggaaag agaaacagta aaaattgcca cagatcgcta atctggacca
    31321 tcagaccaaa tttgaccaca aagctgaata gcgaaagtaa caccagcgtg cgcatcagaa
    31381 gtgacaacga cttaaacggt gctgcgacgg cggagtagag tacgcagcta attgctgcca
    31441 gcgttaatgc attggcaccg gcatcccatt gcgaagcaat actccatgcg ccaatcatca
    31501 tcaacgtaca aaatgtacgc agcccgctcc acatagcttc ggcgttatcg gtatgacgag
    31561 ccaggccgga tgtccgtggg agtctggtat gatcatctac accgctttgc agacgatgta
    31621 attcctgact actttgcaga taaaggcggc aaaaataacg tagtcgctgc cagaaggcga
    31681 cgtgccgata gtcggcgacg ttggtcgggc gtagcggggc gataatacgt gcgacggtgt
    31741 aaacatctgt ttgcgaactg gcgagcgccg tcagcaactg ttcgagaatt tctcgtgtgg
    31801 cacctggcgg tgagggccag ttgagcaaca tacgtcgcag gctagagatg acactggtca
    31861 tacgtaattg ctggtggagc agcgcattaa ggcgcgcgtt ttgctggcga aaacgatagt
    31921 ggctccagaa agcctggata cgcagcaaat tcatggtcag tatctgccca atcacccctt
    31981 catgtgctgc acgaatggca tcggttgttt caggctgcca gagtaaactg gcatgttcaa
    32041 gtagtcgggc gtgcatgttt ttcaatgcgg ttaaaagggc tgtagcatcg gaactgctag
    32101 gcaggatcat catcatcatg ccgccgcaca aaatgccgac aatcacctcg caaacgcgcg
    32161 cctgagcgat atcccacagc tggctggcct cagtaatatt aaccatcgga aaggcgatga
    32221 tggcagccgt gtagcccgcc agttgaaatg catacgcgac gttattcgtg aagtgcgcac
    32281 aggcccaggt acaaaagcca agccacgccg acatgctcaa tagaaaaaac cacggctcat
    32341 tgagcgtatg cccggcaaga agcagtgccg caatggctcc gagcaaactg ccagcgatgc
    32401 gtccgaggct tttgctgata acaccgccaa cggtgggaaa gctaaccact gcagccgagg
    32461 tcatcgccca atagggttca tccagattta aataataggc aaccgtcagc gccagacaca
    32521 tggcaatggt attgcgtaac gcataacgcc attgcgccag cgtggccctg aaccagggca
    32581 aattgcgcaa ggaccatgac gatgcgttca ttattgacct acggcaatgc tacaggtggt
    32641 gcccgacacc aacgtgacat cgcctgggac tttatccagc gcaaagcgga cgggaacgcg
    32701 ttgggcgagt cgaacccagg ggacgttagg tttaacatcc ggaataaggc ttgagtcact
    32761 ttcaacgctc tgatcataaa tcgcccgacc gatactggaa acgtgaccct gtaacgtttt
    32821 gttatcacta tagagggtaa tttgtgctgg tgcaccttcg cggatatggc gcaacttggt
    32881 ttcttcaaaa taaccaatga cgtaaaacga gtggctgtcg acaagggcaa acagcggttt
    32941 cccggtgtca gcgtaatcgc cgatgcgggt agtgaggtta gtcacccaac cactcaccgg
    33001 ggcgcgaatt tccgtttgcg ccagttgcca ttgtgtctgt ttcagcgtgg cctgtgcggc
    33061 attaacactg gcctgcatcg ctttaacatt gaggttagcg gtatcgagtt cttcggcaga
    33121 aataaaattt tgtgagagat gacgacgacg attcgcttcg ttattggctt ttgcgaggtc
    33181 agattgcgct ttcgccagct gagcctgtgc gttcagctcg gcgatctgaa agggcgtttt
    33241 atcgatcgtg agcagaagat cccctgcttt aaccagctgg ttgtctttta tattcagctc
    33301 aacaatacgt cccgacacct gtggggtgat agaaacctgt tctgcgcgta ttttcccgtc
    33361 gcgtgtccac ggagattgca tgtaataatt ccacagccac catccggcaa gaaccgcaac
    33421 taccgctaca atgattgttg agaaatactt aattgtttta attgacatag ttaccacgca
    33481 atcagtattg ccagagcaag gcaaacgcaa atcgcaaaca gcgataaatc cattaacaag
    33541 ggatgccaga tgtcaccggc gtagatccag ccacgaagca ggcggtgtac gacaagccag
    33601 atgacgaatc caaacgcgaa ggctttgaaa aacggaggaa agtagacgga cgcactgaac
    33661 accagatctt gtaagggcaa tcctgttgca ttgagcataa acttcacaaa gtagattcct
    33721 ttacgaccga atatattgcg ttagattaat aaatattctt taagtgcgaa aaatttacgc
    33781 gcaatttctg aaaaatatgt ctgacggtaa ccaaatgcag caatacattt gttttggcaa
    33841 tacaattgct gcacactatt ctaaaagccg cataatatct tagcaagcta attataagga
    33901 gatgaaattg gaatcgccac taggttctga tctggcacgg ttggtgcgca tatggcgtgc
    33961 tctgatagac catcgcctga aaccgctgga gttaacacaa acccattggg ttacgttaca
    34021 caatatccat cagttacctc cagaccagtc gcaaattcaa ctggcaaaag cgattggcat
    34081 cgagcagcca tcactggtcc gtactctgga ccaactggaa gaaaaagggt taatttcgcg
    34141 tcaaacttgt gccagcgatc gtcgggctaa acgtattaaa ctgacggaaa aggcagagcc
    34201 actgatcagc gaaatggaag ctgttattaa caaaacccgc gcggaaatat tacatggcat
    34261 ctccgcagag gaactggagc aactgattac gctcatcgca aaacttgagc ataatatcat
    34321 tgagttacag gccaaagggt gaaatgaagg gggcgtgtgg tcagtaacct gaccacacgc
    34381 aaacttattt agcgcggaga aacggtcacc tgactgccat tgctggccag tacgacacgt
    34441 tggcccggag agaaacgagt gttgccttgt ttctgtacca ccatgatggt attaccatcg
    34501 tctttacgaa tttccagctc gacaccctgc gttttgttca ttgcactctg tacgccctga
    34561 ccagctacgc cacctgcaac agcgcctgct gcagtagcca gagaacgccc ggttccgcca
    34621 ccaacagtat tccccaggaa accaccaaga acagcaccgc caattgcacc gataacgttg
    34681 gaatcatcac cgccctgaat ctgtaccgga cgtacgttaa cgatggtgcc atagctgaca
    34741 ttctgtactt gtttcgcttc agaagcggta taaacatccc ctgacagggt gtcgttatta
    34801 acacaaccga caagagacag acctaccatt gaaacaacca atacgcgttt aatcattgaa
    34861 aaatctcctg ttcaccatga aacgccacgc aagcatccct catggtcaaa gtatatggca
    34921 tatgtgaatc catggttaca tgttctacca aatatgagca aatcataatg aaagtaggat
    34981 caaccaatta taaacaaaac attcatgatg attattgatt tgaagataat gttttactac
    35041 caacggtaaa aatcggtcat tccgcatggt attcacgaaa agtttatgtt tgagtggtgc
    35101 gcaaacgtca gattgaaaaa ggatgaacta tgaaatcggg ccgctttatt ggcgttatgt
    35161 caggcaccag ccttgatggt gttgatgttg tgttggcgac aatagatgaa caccgggtcg
    35221 cacagctggc aagcttgagc tggccgatcc cggtgtctct gaaacaggct gtactggata
    35281 tttgccaggg ccagcagctt acactttcgc agtttggaca gcttgatact caactcgggc
    35341 gactttttgc tgatgcggtc aatgccttgc ttaaggaaca aaacctgcag gcgagagata
    35401 tagttgcgat cggctgtcac ggtcaaaccg tctggcatga accgacgggc gtggcaccac
    35461 acactttaca gattggcgat aacaatcaaa ttgtggcacg caccggaatt acggttgtcg
    35521 gtgattttcg ccgtcgcgat attgccttgg gaggacaagg cgcaccgctg gtacctgcgt
    35581 tccatcatgc cttgctggct cactcaaccg agcgacgaat ggtgctcaat attggcggca
    35641 tcgccaatct gtcactgctc attcctgggc agccggttgg cggctacgat accggtcctg
    35701 gtaacatgct gatggatgcc tggatctggc gtcaggccgg taaaccttac gataaagatg
    35761 ccgagtgggc acgggcgggt aaagttattc tcccactgct gcaaaatatg ctcagcgacc
    35821 catatttctc gcaacctgca ccgaaaagca ccggacgcga atactttaac tacggctggc
    35881 tggagcgcca tttacgccat tttccgggtg ttgatccccg tgatgttcag gccacactgg
    35941 cagaactcac cgccgtgacc atttctgaac aagttttgtt gagcggtggc tgcgaacgat
    36001 tgatggtatg tggtggaggt agtcgtaatc cgctactcat ggcgcgtctg gcggcattac
    36061 tgccaggcac agaagtcacc accaccgatg ccgttggcat tagtggcgat gacatggaag
    36121 cattggcttt cgcctggctt gcctggcgga cgctggcggg attaccagga aatctgcctt
    36181 ccgtcactgg cgcaagccag gagacggtac tgggggctat tttccctgct aacccgtgat
    36241 atcagagtta actgaatttt tcttaccgcg tctgccgtta cacttggaac gttagggagg
    36301 gcgtattgcc ctccagacca ggaaagtctt cgggaaatgt ctatgaaaaa actgttactg
    36361 atctgtttgc cggtgcttct caccggctgt agcgcgttca atcaactggt tgagcacatg
    36421 caaaccgcta cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa actgaataat
    36481 ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa acagggcatt
    36541 tcagcgtctg gtgcacgtta cactgacgga atctatgttt tctggtcgaa aggcgatgaa
    36601 gcgactgtct ataaacgcga ccgcatcgta ttgaataact gtcagttaca aaatccacag
    36661 cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat ccgtaaagaa
    36721 aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg aatacaccaa
    36781 aggcgggtta cgccgtcgcg atcttcccgc cgatccatta accctttttg aacgttggct
    36841 ctctcaggcg tgtgaagcca aactggcaga ccctaccgcg atggtggtcg ctaccgtgga
    36901 tgaacatgct cagccttatc aacgcatcgt tttactcaaa cattacgacg aaaaaggcat
    36961 ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata atccgcgcgt
    37021 tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg gtaaagcaga
    37081 acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg atagccagat
    37141 tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc ttgaaagtaa
    37201 attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga gcttttgggg
    37261 cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc atcgcctgca
    37321 tgaccgcttt ttgtaccagc gagaaaatga tgcgtggaag attgatcgtc ttgcaccctg
    37381 aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac tttattctat
    37441 gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt tatatacatg
    37501 gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg gctggtagcc
    37561 caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat cgcgctctat
    37621 tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc attgttatgc
    37681 ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg cgcgacgggt
    37741 ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga agaaactgtt
    37801 caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt cgactgtgga
    37861 gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa tgtgctgacc
    37921 ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa agaagcggtt
    37981 aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc ctacaacctg
    38041 ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg gtgtggtgct gcaaattggt
    38101 ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg tctgcatcag
    38161 aatcaggtgt ttggcctgac cgttccgctg atcactaaag cagatggcac caaatttggt
    38221 aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta caaattctac
    38281 cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt cttcaccttt
    38341 atgagcattg aagagatcaa cgccctggaa gaagaagata aaaacagcgg taaagcaccg
    38401 cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga agaaggttta
    38461 caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc gctgagtgaa
    38521 gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga aaagggcgca
    38581 gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca ggcgcgtaaa
    38641 actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc tgaatacttc
    38701 tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg caaaaagaat
    38761 tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag aaatcactcc
    38821 ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt ctcacgttgt
    38881 ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg gcgcgaacgt
    38941 ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat ggactggctg
    39001 cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca ttgataaatt
    39061 acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg gtgaacatat
    39121 cctcggtatc gtccgtcagg tgaaagccgc gaatccgcag gcgaaatatt tttgcgatcc
    39181 ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag agtttcatgt
    39241 gcggcacggt ttgcctgcca gcgatatcat tgcgccaaat ctggttgagc tggaaatact
    39301 ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg aactcattgc
    39361 gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca gccgtgaccg
    39421 ttttgaaatg ctgctggtca ccgccgatga agcctggcat atcagccgtc cgctggtgga
    39481 ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac tgctggtgaa
    39541 actgcttcag ggggcaacgc tgcaggaggc gctggaacat gtgaccgctg cagtctacga
    39601 aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg ctgctcagga
    39661 tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata tctcatttcg
    39721 gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga cagcgcgtct
    39781 tgtacttccg gacgttcagc catacgttgc ataaatgctg caatgtgctc aaacccttcc
    39841 agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc atcagcaatt
    39901 gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac atattgcagc
    39961 ttcttctcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc agggcgaaac
    40021 agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc gatggtttta
    40081 tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct gtcggcaaga
    40141 tactgcataa tcgctacgcc ttccgtcagc aaagtaccgt catccagcag caatgcaggc
    40201 acctgcccct tagggttaac ggcaaagtaa tcgtcaccgt tttcgagacg ttttttcatt
    40261 aaatccacac tgacgagggt aaaatccttt ccgctctcac gcagggtgat atgggaagcg
    40321 agagagcagg cacccggtat gtagaacaat ttcatcggta actccttttg tctgtggttt
    40381 aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt tagcggcttg
    40441 taaaagagtt tccctgaatt ttacgctacg gctgctttcg ccgctttgtc tgcagcgtca
    40501 tcctgcgtca tgcggtgcag tttcggcgcg gtcagcagca tcagtactgc aataacggca
    40561 gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat cagcggatcg
    40621 gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag gtttgcaccg
    40681 gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg aacgagttga
    40741 gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg caggccatag
    40801 cttgcgacca gccagcttac agacacgata ccagcgtcag acgcgaattt cgcacccagc
    40861 ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt ggttggcatc
    40921 ggcagggtat cgcccatctt gttatagata gcggccagaa tcggactacc gatgatgatc
    40981 cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag aatggagtgc
    41041 tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta cagcacgaag
    41101 aagataatgg cttcgagcat caggatgaag gcaacgatca ttttacgacg cgcagcacct
    41161 ttcatcgcga aggcttcttt accgaagata accacgatac cgaaggcaac aacgcccagc
    41221 gccatacgcg caacttcctg attgtgcagc agccaggtgg cgatagcgat cagtgccaca
    41281 acaccaataa tggtcagcag caggttacgg tagttgatag gctcgaagtc tggttttgaa
    41341 ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat cagcaggcct
    41401 acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca cggcgtagca
    41461 atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa tgcaccgtcc
    41521 agacgcgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt agctttaaac
    41581 aggccgttac cgaccgcaat agccgccata cccatataaa cgataccggc gtcgtgacca
    41641 gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat aattacgcgt
    41701 ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc ataaaccagg
    41761 gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag ttgtttaacc
    41821 aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca taactcaatc
    41881 gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac gctttcagtt
    41941 ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat taacgggggt
    42001 gctttctcgc gttatgtata aaaaacatac gcgcaattgt tatagtggga ggggaaacgg
    42061 caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc tgttacatat
    42121 aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa tttttcaaaa
    42181 aacgtaacgg cagatttttt cactgcactg agaggggggt gatggcttaa cggcgatatg
    42241 ttctgctatt tagcgttttt actagtgaga tagtccagtt tctgaaaaat agcc
//
LOCUS       DBANUU010000026        41666 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000026 DBANUU010000000
VERSION     DBANUU010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.026"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            253..339
                     /gene="leuL"
                     /locus_tag="ACIE68_001351"
     CDS             253..339
                     /gene="leuL"
                     /locus_tag="ACIE68_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFX5907695.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            432..2003
                     /gene="leuA"
                     /locus_tag="ACIE68_001352"
     CDS             432..2003
                     /gene="leuA"
                     /locus_tag="ACIE68_001352"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFX5907696.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2003..3094
                     /gene="leuB"
                     /locus_tag="ACIE68_001353"
     CDS             2003..3094
                     /gene="leuB"
                     /locus_tag="ACIE68_001353"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFX5907697.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3097..4497
                     /gene="leuC"
                     /locus_tag="ACIE68_001354"
     CDS             3097..4497
                     /gene="leuC"
                     /locus_tag="ACIE68_001354"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFX5907698.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4508..5113
                     /gene="leuD"
                     /locus_tag="ACIE68_001355"
     CDS             4508..5113
                     /gene="leuD"
                     /locus_tag="ACIE68_001355"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFX5907699.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5162..6340)
                     /gene="setA"
                     /locus_tag="ACIE68_001356"
     CDS             complement(5162..6340)
                     /gene="setA"
                     /locus_tag="ACIE68_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFX5907700.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLILFHSRLALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVIALFLTAKVKDV
                     "
     gene            complement(6442..6573)
                     /gene="sgrT"
                     /locus_tag="ACIE68_001357"
     CDS             complement(6442..6573)
                     /gene="sgrT"
                     /locus_tag="ACIE68_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFX5907701.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6662..8317
                     /gene="sgrR"
                     /locus_tag="ACIE68_001358"
     CDS             6662..8317
                     /gene="sgrR"
                     /locus_tag="ACIE68_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFX5907702.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLIPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEIDYDQWHTGEIES
                     DIWLNSANFTLPLDFSVFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLLHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8351..8450
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8481..9464
                     /gene="thiB"
                     /locus_tag="ACIE68_001359"
     CDS             8481..9464
                     /gene="thiB"
                     /locus_tag="ACIE68_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFX5907703.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAINVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDTPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9440..11050
                     /gene="thiP"
                     /locus_tag="ACIE68_001360"
     CDS             9440..11050
                     /gene="thiP"
                     /locus_tag="ACIE68_001360"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFX5907704.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AIWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLATLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGLNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11034..11732
                     /gene="thiQ"
                     /locus_tag="ACIE68_001361"
     CDS             11034..11732
                     /gene="thiQ"
                     /locus_tag="ACIE68_001361"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFX5907705.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11846..12610)
                     /gene="yabI"
                     /locus_tag="ACIE68_001362"
     CDS             complement(11846..12610)
                     /gene="yabI"
                     /locus_tag="ACIE68_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFX5907706.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12696..13574)
                     /gene="araC"
                     /locus_tag="ACIE68_001363"
     CDS             complement(12696..13574)
                     /gene="araC"
                     /locus_tag="ACIE68_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFX5907707.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13913..15613
                     /gene="araB"
                     /locus_tag="ACIE68_001364"
     CDS             13913..15613
                     /gene="araB"
                     /locus_tag="ACIE68_001364"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFX5907708.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLSWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRSPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15624..17126
                     /gene="araA"
                     /locus_tag="ACIE68_001365"
     CDS             15624..17126
                     /gene="araA"
                     /locus_tag="ACIE68_001365"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFX5907709.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHTLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17411..18106
                     /gene="araD"
                     /locus_tag="ACIE68_001366"
     CDS             17411..18106
                     /gene="araD"
                     /locus_tag="ACIE68_001366"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFX5907710.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGAKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18181..20532
                     /gene="polB"
                     /locus_tag="ACIE68_001367"
     CDS             18181..20532
                     /gene="polB"
                     /locus_tag="ACIE68_001367"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFX5907711.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVFLAPQESVAF
                     IPADQVPRAQHILRGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMYNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQSKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20696..23602
                     /gene="rapA"
                     /locus_tag="ACIE68_001368"
     CDS             20696..23602
                     /gene="rapA"
                     /locus_tag="ACIE68_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFX5907712.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23614..24273
                     /gene="rluA"
                     /locus_tag="ACIE68_001369"
     CDS             23614..24273
                     /gene="rluA"
                     /locus_tag="ACIE68_001369"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFX5907713.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24389..25204)
                     /gene="djlA"
                     /locus_tag="ACIE68_001370"
     CDS             complement(24389..25204)
                     /gene="djlA"
                     /locus_tag="ACIE68_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFX5907714.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25459..27828
                     /gene="lptD"
                     /locus_tag="ACIE68_001371"
     CDS             25459..27828
                     /gene="lptD"
                     /locus_tag="ACIE68_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFX5907715.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSNPQNNKLADSVNRVMPQFKVDGKMVFERDMNLLAPGFTQT
                     LEPRAQYLYVPYRDQSDIYNYDSALLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSR
                     VYDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLR
                     GGIQYDTRLDNVATSSSSIEYRRDEDRLVQLNYRYASPEYIQATLPRDSRDKRWNAPQ
                     YKDGISQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERK
                     LNGWDNDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27881..29167
                     /gene="surA"
                     /locus_tag="ACIE68_001372"
     CDS             27881..29167
                     /gene="surA"
                     /locus_tag="ACIE68_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFX5907716.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARTIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29167..30156
                     /gene="pdxA"
                     /locus_tag="ACIE68_001373"
     CDS             29167..30156
                     /gene="pdxA"
                     /locus_tag="ACIE68_001373"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFX5907717.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNFAIKMIVNTQ"
     gene            30153..30974
                     /gene="rsmA"
                     /locus_tag="ACIE68_001374"
     CDS             30153..30974
                     /gene="rsmA"
                     /locus_tag="ACIE68_001374"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFX5907718.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30977..31354
                     /gene="apaG"
                     /locus_tag="ACIE68_001375"
     CDS             30977..31354
                     /gene="apaG"
                     /locus_tag="ACIE68_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFX5907719.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31361..32209
                     /gene="apaH"
                     /locus_tag="ACIE68_001376"
     CDS             31361..32209
                     /gene="apaH"
                     /locus_tag="ACIE68_001376"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFX5907720.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            32234..32833
                     /locus_tag="ACIE68_001377"
     CDS             32234..32833
                     /locus_tag="ACIE68_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HFX5907721.1"
                     /translation="MADYFEIRADYNQHTITVYQAYNDAIADVAVRDGRFDAPFSFNR
                     MTWIKPSFMWMMERSNWGLKKDQQRILAIRIKRTFFDTLLEQAELTTPEAHVYPHAGV
                     WETLFAQANVYVQWDPERSINGKKLEHRSLQLGISRNLISQFNEDAIVAIDDLTPLVR
                     KCHNLLINGKTTQAKSFLPPEKIYPVSAAARKALGMKNS"
     gene            32869..33336
                     /locus_tag="ACIE68_001378"
     CDS             32869..33336
                     /locus_tag="ACIE68_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5907722.1"
                     /translation="MRQINAGLNTREIILEMIDEADEPPFAEQRTWHRKRIAFLCDCI
                     SVAVDYGFFCSVSQQSIMNITRMAEDVWITESKTTQIIDIWQQIKRLYPPNIIPCTQQ
                     LPAKELLELLLSSPDDLRIIEIPDIIGDVFDDQTWIRLFKKHFADIILLRCNV"
     gene            complement(33435..33614)
                     /locus_tag="ACIE68_001379"
     CDS             complement(33435..33614)
                     /locus_tag="ACIE68_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFX5907723.1"
                     /translation="MNDKTSDFTSERDFVSRAHCVGLNSQATLQWTEVNEDAISALNE
                     LAEETGCFSDEYRKF"
     gene            complement(33635..34114)
                     /gene="folA"
                     /locus_tag="ACIE68_001380"
     CDS             complement(33635..34114)
                     /gene="folA"
                     /locus_tag="ACIE68_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFX5907724.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34306..36168)
                     /gene="kefC"
                     /locus_tag="ACIE68_001381"
     CDS             complement(34306..36168)
                     /gene="kefC"
                     /locus_tag="ACIE68_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFX5907725.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36161..36691)
                     /gene="kefF"
                     /locus_tag="ACIE68_001382"
     CDS             complement(36161..36691)
                     /gene="kefF"
                     /locus_tag="ACIE68_001382"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFX5907726.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVLQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(36802..38133)
                     /gene="yaaU"
                     /locus_tag="ACIE68_001383"
     CDS             complement(36802..38133)
                     /gene="yaaU"
                     /locus_tag="ACIE68_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5907727.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPTLKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATVF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38191..38478)
                     /gene="fixX"
                     /locus_tag="ACIE68_001384"
     CDS             complement(38191..38478)
                     /gene="fixX"
                     /locus_tag="ACIE68_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFX5907728.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVELRYG"
     gene            complement(38475..39761)
                     /gene="fixC"
                     /locus_tag="ACIE68_001385"
     CDS             complement(38475..39761)
                     /gene="fixC"
                     /locus_tag="ACIE68_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFX5907729.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(39812..40753)
                     /gene="fixB"
                     /locus_tag="ACIE68_001386"
     CDS             complement(39812..40753)
                     /gene="fixB"
                     /locus_tag="ACIE68_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFX5907730.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAVVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGEMHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(40768..41538)
                     /gene="fixA"
                     /locus_tag="ACIE68_001387"
     CDS             complement(40768..41538)
                     /gene="fixA"
                     /locus_tag="ACIE68_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFX5907731.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT        10098 a        11408 c        10894 g         9266 t
ORIGIN      
        1 aacgcgtgca ttttatcccc cttttcctcg ttgattagat gcaaaaattt attctgaaat
       61 atgtcaaccg atgaaaagcg tcggtggtta agcagaaatt aatatcgctt gttttaacca
      121 ccgcagcaca attagctaat ttgacgaatg cagaactcac tctggcggaa cgtttttgtt
      181 gcgtcagggt tgacatccgt ttttgtatcc agtaactcta aaagcatatc gtattcatct
      241 ggagctgatt taatgactca catcgttcgc tttatcggtc tactactact aaacgcatct
      301 tctttgcgcg gtagacgagt gagcggcatt cagcattaag ccagcacgca gtcaaacaaa
      361 aaacccgcgc cattgcgcgg gtttttttat gcccgaagcg aggcgctcaa aaagagacaa
      421 ggacccaaac catgagccag caagttatta ttttcgatac cacattgcgc gacggtgaac
      481 aggcgttaca ggcaagcttg agtgtgaaag aaaaactgca aattgcgctg gcccttgagc
      541 gtatgggtgt tgacgtgatg gaagtcggtt tccccgtctc ttcgccgggc gatttcgaat
      601 cggtgcagac catcgcccgc caggtcaaaa acagccgcgt atgtgcgtta gctcgctgcg
      661 tggaaaagga tatcgacgtg gcagctgaat ctctgaaagt tgccgaagcc ttccgtatcc
      721 atacctttat tgccacttca ccaatgcaca ttgccaccaa gctgcgtagc acgctggacg
      781 aagtaatcga acgcgctatc tatatggtga aacgcgcccg taattacacc gatgatgttg
      841 aattttcttg cgaagatgcc ggacgcacac ccattgccga tctggcgcgc gtggttgaag
      901 cggcgattaa cgccggtgcc accaccatca acattccgga caccgtgggc tacaccatgc
      961 cgtttgagtt cgctggaatc atcagcggcc tgtatgaacg cgtgcctaac atcgacaaag
     1021 ccattatctc cgtacatacc cacgacgatt taggcctggc agttggcaac tcactggcgg
     1081 cggtacatgc cggagcgcgc caggtggaag gtgcaatgaa tgggatcggc gagcgagccg
     1141 gtaactgttc gctggaagaa gtgatcatgg cgatcaaagt tcgtaaggat attctcaacg
     1201 ttcataccgc cattaatcac caggagatat ggcgcaccag ccagttagtt agccagattt
     1261 gtaatatgcc gatcccggca aacaaagcca ttgttggcag cggcgcattc gcacactcct
     1321 ccggtatcca ccaggatggt gtactgaaaa accgtgaaaa ctacgaaatc atgacaccag
     1381 aatctattgg tctgaaccaa atccagctga atctgacctc tcgttcgggg cgtgcggcgg
     1441 tgaaacatcg catggatgag atgggatata aagaaagtga atataattta gacaacctgt
     1501 acgacgcttt cctgaagctg gcggacaaaa aaggccaggt gtttgattac gatctggagg
     1561 cgctggcctt catcggtaag cagcaagaag agccggagca tttccgtctg gattacttca
     1621 gcgtgcagtc aggttctaac gatattgcca ctgccgccgt caaactggcc tgtggcgaag
     1681 aagtcaaagc agaagccgcc aacggtaacg gtccggtcga tgccgtctat caggcgatta
     1741 accgcatcac tgactataac gtcgaactgg tgaaatacag cctgaccgcc aaaggtcacg
     1801 gtaaagatgc gctgggtcag gtggatattg tcgccaacta caatggtcgc cgcttccacg
     1861 gcgtcggcct ggccaccgat attgtcgagt cctccgccaa agccatggtg cacgtactga
     1921 acaatatctg gcgtgccgca gaagtcgaaa aagagttgca acgcaaagct caacacaacg
     1981 aaaacaacaa ggaaaccgtg tgatgtcgaa gaattaccat attgccgtat tgccggggga
     2041 cggtattggt ccggaagtga tgacccaggc gctgaaagtg ctggatgccg tgcgcaaccg
     2101 ctttgcgatg cgtatcacca ccagccatta cgatgtaggc ggcgcagcca ttgataacca
     2161 cgggcagccg ctgccgcctg cgacggttga aggttgtgag caagccgatg ccgtgctgtt
     2221 tggctcggta ggtggtccga agtgggaaca tttgccgcca gaccagcaac cagaacgcgg
     2281 cgcactgttg cctttgcgta aacacttcaa attattcagc aatctgcgtc cggcaaaact
     2341 gtatcagggg ctggaagcat tctgtccgct gcgtgctgac attgccgcta acggcttcga
     2401 catcctgtgt gtgcgcgaac tgaccggcgg catctatttc ggtcagccaa aaggccgcga
     2461 aggtagcgga caatatgaaa aagcgtttga taccgaggtg tatcaccgtt ttgagattga
     2521 acgtatcgcc cgcatcgcgt ttgaatctgc tcgtaaacgt cgccataaag tgacgtctat
     2581 cgacaaagcc aacgtgctgc aatcctctat tttatggcgg gagatcgtta acgagatcgc
     2641 cacggaatac ccggatgtcg aactggcgca tatgtacatc gacaacgcca ccatgcagct
     2701 gattaaagat ccatcacagt ttgacgttct gctgtgctcc aacctgtttg gcgacattct
     2761 gtctgacgag tgcgcaatga tcactggctc aatggggatg ttgccttccg ccagcctgaa
     2821 cgagcaaggt tttggtctgt atgaaccggc gggcggctcg gcacccgata tcgcaggtaa
     2881 aaatatcgcc aacccgattg cacaaattct gtcgctggcg ctgctgctgc gttacagcct
     2941 ggatgccgat gatgcggctt ccgccattga acgcgccatt aaccgcgcat tagaagaagg
     3001 cattcgcacc ggggatttag cccgtggcgc tgccgccgtt agtaccgatg aaatgggcga
     3061 tatcattgcc cgctatgtgg cagaaggggt gtaatcatgg ctaagacgtt atacgaaaaa
     3121 ttattcgacg ctcacgtagt gtacgaagcc gaaaacgaaa ccccgctgtt atatatcgac
     3181 cgccacctgg tgcatgaagt gacctcaccg caggcgttcg atggtctgcg cgcccacggt
     3241 cgcccggtac gtcagccggg gaaaaccttc gccaccatgg atcacaacgt ctctactcag
     3301 accaaagaca ttaatgcctg cggtgaaatg gcgcgcatcc agatgcagga gttgatcaaa
     3361 aactgcaaag aatttggcgt cgagctgtat gacctgaatc acccgtatca ggggatcgtc
     3421 cacgtaatgg ggccggaaca gggcgtcaca ttgccgggga tgaccattgt ctgcggcgac
     3481 tcacataccg ccacccacgg cgcgtttggc gcactggcct ttggtatcgg cacttccgaa
     3541 gttgaacacg tactggcaac gcaaaccctg aaacagggtc gtgcgaagac catgaaaatt
     3601 gaagtccagg gcaaagccgc gccgggcatt accgcgaaag atatcgtgct ggcgattatc
     3661 ggtaaaactg gtagcgcagg cggcaccggg catgtggtgg agttttgcgg cgaagcaatc
     3721 cgtgatttaa gcatggaagg tcgtatgacc ctgtgcaata tggcaatcga aatgggcgcg
     3781 aaagccggtc tggttgcacc ggacgaaact acctttaact atgtcaaagg ccgtctgcat
     3841 gcgccgaaag gcaaagattt cgacgacgcc gtcgcctact ggaaaaccct gcaaaccgac
     3901 gaaggcgcaa ctttcgatac cgttgtcact ctgcaagcag aagagatttc gccgcaggtc
     3961 acctggggca ccaatccagg ccaggtgatt tccgtgaacg acaatattcc cgatccggct
     4021 tcgtttgccg atccggttga acgcgcgtcg gcagaaaaag cgctggccta tatggggctg
     4081 aaaccgggta ttccgctgac cgaagtggct atcgataaag tgtttatcgg ttcctgtacc
     4141 aactcgcgca ttgaagattt acgcgcggca gcggaaatcg ccaaagggcg aaaagtcgcg
     4201 ccaggcgtgc aggcactggt ggttcccggc tctggcccgg taaaagccca ggcggaagcg
     4261 gaaggtctgg ataaaatttt tatcgaagcc ggttttgaat ggcgcttgcc tggctgctca
     4321 atgtgtctgg cgatgaataa cgaccgtctg aatccgggcg aacgttgtgc ctccaccagt
     4381 aaccgtaact ttgaaggccg ccaggggcgc ggcgggcgca cgcatctggt cagcccggct
     4441 atggctgccg ccgcggctgt gaccggacat tttgccgaca ttcgcaacat taaataagga
     4501 gcacaccatg gcagagaaat ttatcaaaca tacaggcctg gtggttccac tggatgccgc
     4561 caatgtcgat accgatgcaa tcatcccgaa acagtttttg cagaaagtga cccgtacggg
     4621 ttttggcgcg catctgttta acgactggcg ttttctggat gaaaaaggcc aacagccaaa
     4681 cccggacttc gtgctgaact tcccgcagta tcagggcgct tccattttgc tggcacgaga
     4741 aaacttcggc tgtggctctt cgcgtgagca cgcgccctgg gcattgaccg actacggttt
     4801 taaagtggtg attgcgccga gttttgctga catcttctac ggcaatagct ttaacaacca
     4861 gctgctgccg gtgaaattaa gcgatgcaga agtggacgaa ctgtttgcgc tggtgaaagc
     4921 caatccgggg atccatttcg acgtggatct ggaagcgcaa gaggtgaaag cgggagagaa
     4981 aacctatcgc tttaccatcg atgccttccg ccgccactgc atgatgaacg gtctggacag
     5041 tattgggctt accttgcagc acgacgacgc cattgccgct tatgaagcaa aacaacctgc
     5101 gtttatgaat taatcccctt gcccggtcaa atgaccgggc tttccgctat cgtccacgtc
     5161 atcaaacgtc tttaaccttt gcggttaaaa ataatgcgat aacagaaata accgcaatta
     5221 cccagtagac agcaaagtgc ccccaacttt gtgcaattgc tccctgaata acgccagcca
     5281 gaattacccc ggtagaaata ctgttagtaa ataaggtggt agctgccccc gctcttccag
     5341 gcattaaatc ctgaaaccat agcatcccaa tacccgcaac aatgccgata aatacagcgt
     5401 taaaaagttg cagcgtcatc aacgccagac ggctatgaaa taaaatcaat ccggtgtaaa
     5461 acagtactcc tgccgccact gctatgacca tcattcgccg cttaccataa cgtttgacat
     5521 agtagccagc cagaatcatt gctggtattt ccagaccagc tgccgtcccc atcaggaaac
     5581 ccgccagttt gtctggcaat cctaactcgc tactgatcca caacggcata tcaataatgt
     5641 acatggtgtt gcaggtccac attaacgtcg aggcgacaaa taacatccgt acgttactat
     5701 cctgccagcc accttgcatt gataaagcat tttccgacgg cagttctacc cgcgccacag
     5761 acggaagcat aaatgcaatc aataccagac tgagtgtgaa tatcccggcg gcaatcgaaa
     5821 acatcaccgt aaagccgtaa ttcaacgcca gcataaaggc caacggtgga ccgataaccc
     5881 atgccagaga aagctgcgca cgcatcaccg agctaaacat caccacttct cgcgccgagt
     5941 tatccgcata ttcccgcgcc agagcaaata actgtggcat tgccgtattg gccagagatg
     6001 ccagaagcac accacaggtg ataagcgtca gataatgacg attaaatgca aacaatagcg
     6061 cattgccgat agccatcaaa cagcaaaata taatcagttt tcgccgatcg ccctgactgt
     6121 cagaacgttt tgccaaccag aggcttacgc cgatcccagc aatagcattc accgtataaa
     6181 agagtccgat ccagaaaggt tgcgcgccaa cctcacgact cagaaataag ctcaatgtag
     6241 gagcctgtag cgccccggcc acccccatca taaaagcgac cagcataaat gccgcgtaaa
     6301 caccgttcat acggcgagcc atcgtcatta tccagatcat acgttccctt tttagcgcgg
     6361 cgagaataaa aaaaaccagc aggtataatc tgctggcggg tgattttaca ccaatactca
     6421 gtcacacatg atgcaggcaa gtcaactttc agaattgcgg tcatcccact gcatcagtcc
     6481 ttcaagcatg gttaatcgtt gtgggacgct taaccaacgc aaccagcaca acttcgctgt
     6541 cgcggtaaaa tagtgctgat aaaactgacg catggggcac ctccttgctt catcgttagg
     6601 aattattgac ttaatatagg gaaaataaaa ttgctgtctt ttgcgcagga gttccccttt
     6661 tatgccatct gctcgtctgc aacaacagtt catccgcctg tggcaatgct gcgagggtaa
     6721 atcgcaggac acaacgctca acgaactggc agcgttattg agctgctcgc gtcgtcatat
     6781 gcgcaccctg ctcaacacca tgcaggatcg cggctggctg acgtgggaag cggaagtcgg
     6841 gcgtggtaaa cgctcgcgtc tgacattcct ctataccggg ctggcgcttc agcaacagcg
     6901 ggcggaagac ctgctggagc aggatcgtat cgaccaacta gtgcagttgg ttggcgacaa
     6961 agcgactgtg cggcaaatgc tggtttctca tctgggccgc agcttccgcc aggggcggca
     7021 catcctgcgc gtgctctact atcgtccgtt gcgtaatctg ctacctggca gcgcattgcg
     7081 ccgttccgaa acccatattg cccggcaaat cttcagttcg ctaacgcgca taaatgagga
     7141 aaatggggaa ctggaagcag acatcgccca ccactggcag caaatatcac cgcttcactg
     7201 gcgtttcttt ttgcgtccag gagtccattt tcatcatggt cgtgaactgg aaatggacga
     7261 cgtgatcgcc tctttaaaac gaatcaatac gctgccgctc tattcgcata ttgctgacat
     7321 tgtgtcgccg acgccctgga cgctggatat ccatctcacg caaccggacc gctggttacc
     7381 gttattgctg gggcaagttc cggcgatgat cctgccgcgc gaatgggaaa ccctcagtaa
     7441 ctttgccagc catcccatcg gcaccggtcc gtatgcggtg atccgcaaca gcaccaatca
     7501 actgaaaatt caggcattcg atgacttctt cggttaccgg gcattaatcg acgaagttaa
     7561 cgtctgggtt ctgccggaaa ttgccgacga gccagccgga ggtctgatgc taaaaggtcc
     7621 acagggcgag gaaaaagaga ttgaaagccg cctggaggaa ggttgctact atttactatt
     7681 cgacagccgc acccatcgcg gggcgaatca gcaagtcaga gactgggtaa gctatgtgct
     7741 ttctccaact aatctggtct atttcgctga ggaacagtac cagcaactgt ggttcccggc
     7801 ttatggactg atcccccgtt ggcaccatgc ccgcaccata aagagcgaaa aaccggctgg
     7861 tctggaaagc ctcaccctga ccttttatca ggatcacagt gagcatcggg tgattgccgg
     7921 gatcatgcag cagattctgg caagccacca ggtcacgctg gaaatcaaag agatcgacta
     7981 cgatcagtgg catacaggag agatcgaaag tgatatctgg ctaaacagcg ccaactttac
     8041 cctgccgctg gacttctctg ttttcgcaca tttatgcgaa gtgccactgc tacaacattg
     8101 cattcccatt gactggcaag ccgacgctgc tcgctggcgc aatggcgaga tgaatctggc
     8161 gaactggtgc cagcaactgg tcgccagcaa agcgatggtg ccattattgc accactggct
     8221 gatcattcag gggcaacgca gtatgcgcgg cctgcgcatg aacaccctcg gctggttcga
     8281 ttttaaatca gcgtggtttg cgccaccgga tccatgattg ctggttgata acaaaatcac
     8341 tacactaacg ccgttctcaa cggggtgcca cgcggatgcg tgcgctgaga aaatacccgt
     8401 cgaacctgat ccggataacg ccggcgaagg gatttgaggc tccttctcaa gtcctttgcc
     8461 actctttttt gaggtgcaaa gtgttaaaaa aatgtctgcc cctgctgttg ctgtgcacag
     8521 cgcccgtttt cgctaaaccc gttctgactg tttataccta cgattccttc gccgccgact
     8581 gggggcctgg tccggtggtt aaaaaagcct ttgaagccga ctgtaattgc gaactgaaac
     8641 tggtggcgct ggaagatggc gtttcgcttc tcaaccgtct acggatggaa ggcaaaaaca
     8701 gcaaagccga tgtagttctg gggctggata acaacctgtt agacgccgcc agtaaaaccg
     8761 gactgtttgc caaaagcggc gtggcagcgg atgccattaa cgttcccggc ggctggaata
     8821 atgacacttt cgtaccgttt gattacggct acttcgcctt cgtctacgac aagaacaaac
     8881 tgaaaaaccc gccccaaagc ctgaaagagc tggttgagag cgatcaaaac tggcgagtga
     8941 tttatcagga tccgcgcacc agtacgccgg ggctgggtct gctgttgtgg atgcaaaaag
     9001 tctatggcga tgacaccccg caggcctggc agaaactggc gaagaaaact gtcaccgtca
     9061 ccaaaggctg gagcgaagcc tacggcctgt ttttaaaagg tgaaagcgat ctggtactga
     9121 gttacaccac ctctccggct tatcacattc tcgaagagaa aaaagataac tacgccgccg
     9181 cgaactttag cgaaggtcac tatctgcagg tggaagtcgc cgcccgcacc gctgccagca
     9241 agcagccgga gctggcgcaa aaatttctcc agtttatggt ttctccggct ttccagaatg
     9301 cgatcccaac cggcaactgg atgtatccgg tggcaaatgt cacgctgcct gccgggtttg
     9361 aacaattgac caaaccagca accacgctgg agttcacgcc agccgaagtg gcggcacaac
     9421 gtcaggcatg gattagcgaa tggcaacgcg ccgtcagccg ttaattcccg gctggttaat
     9481 tccaggtgta agcgccgcca cgctggtggt agcggttgcg ctcgcggcgt ttctcgccct
     9541 gtggtggaac gcgccgcagg gtaactgggt ggcaatctgg caggacagct acctgtggca
     9601 tgtggtgcgc ttctccttct ggcaggcgtt tctctcggcg ctgctctctg tcgtacccgc
     9661 gatattcctc gcccgcgcac tctatcgcag gcgctttccg ggtcggctgg cgctgttgcg
     9721 tctgtgcgca atgaccttga tcctcccggt gctggttgct gttttcggca ttcttagcgt
     9781 ctatggtcgc cagggctggc tggcaacact ctgccaatcg ctcggtctgg agtggacctt
     9841 ttcgccctac ggcctgcaag gtattttgct ggcgcacgta ttttttaatc taccgatggc
     9901 gagccgctta ttactccagg cactggaaaa cattcccggc gaacaacgtc agcttgccgc
     9961 ccagcttggg atgcgcggct ggcatttttt ccgcttcgtc gaatggccgt ggttacggcg
    10021 acaaatcccg ccggttgctg cacttatctt tatgctctgt ttcgccagct tcgccaccgt
    10081 gctgtcgctg ggcggcggtc cgcaggcaac cactatcgag ctggcaatct atcaggcgct
    10141 gagttacgac tacgatcctg cccgcgcggc gatgctggcg ctgatccaga tggtgtgttg
    10201 cctcgggttg gtgctgctga gtcagcgatt gagtaaggcc attgcgccag gcaccacgct
    10261 gctgcaaggc tggcgcgacc cggacgatcg tctgcatagc cgcatttgcg acacggtgtt
    10321 aattgtgctg gcgctgctgc tgttgctgcc accgttgctg gcggtgatcg tcgatgggct
    10381 aaatcgccag ttgccggaag tgctggcaca accggtgctg tggcaggcgc tgtggacctc
    10441 gttgcgtatt gcgctggcgg caggtgtatt gtgcgtagtg ctgaccatga tgctgctatg
    10501 gagcagtcgc gaacttcggg cgcggcagaa aatgctggcg ggtcaggcgc tggagatgag
    10561 cggcatgttg atcctcgcca tgccggggat tgtgctggct accggcttct ttttactgct
    10621 caacaacacc attggcctgc cgcaatctgc tgacggcatt gtgattttca ccaatgcgtt
    10681 aatggcgatc ccttatgcgc tgaaagtact ggaaaacccg atgcgcgata tcaccgcccg
    10741 ctacagtatg ttgtgtcagt cgctgggcat tgaaggctgg tcgcgcttaa aagtggtcga
    10801 gctgcgcgcc ctgaaacgtc cactggcgca ggcgctggct tttgcctgcg tgctgtcgat
    10861 tggtgatttt ggcgtagtgg cgttgttcgg taacgatgat ttccgtaccc tgccgtttta
    10921 tctctaccag caaattggct cctatcgcag ccaggacggt gcggtcaccg cgttaattct
    10981 gctactactc tgttttctgc tgtttaccgt gattgaaaaa ctaccggggc gaaatgttaa
    11041 aactgactga tatcacctgg ctttatcacc atttgccgat gcgttttagc ttaacggtag
    11101 aacgcggcga gcaggtggca atcctcgggc caagcggcgc gggtaaaagt accctgctga
    11161 atttgatcgc cggttttctg acgccagcca gcggttcgct gactatcgac ggcgttgatc
    11221 acaccacgac gccgccgtca cgccgtccgg tgtcgatgct gtttcaggaa aacaacctgt
    11281 tcagtcacct gacggtcgcg cagaacatcg ggctggggct aaatccggga ttgaaactga
    11341 acgcggcaca gcaggagaaa atgcacgcta tcgcccgcca gatggggatt gataatttaa
    11401 tggcgcggtt acctggcgag ctttccggcg gtcagcgaca gcgagtggcg ttagcacgtt
    11461 gtctggtacg cgaacagccg attttattgc tcgatgaacc gttctctgcg ctcgatccgg
    11521 cactacgcca ggagatgttg acgctggtga gcacgagctg ccagcagcaa aaaatgacgt
    11581 tattgatggt gtcacacagt gtggaagatg ccgcgcggat cgccacgcgc tcggtggtgg
    11641 tcgccgacgg gcgcatcgcc tggcagggta agaccaatga gttgttgagc ggtaaggcga
    11701 gtgcttcagc actattgggg attacgggtt agtatgcact gtcggatgcg gcgtgaacgc
    11761 cttatccgac ctacgggtag tgcgttttgt aggcctgata agacgcggca agcgtcgcat
    11821 caggcaaccc gcacaagact atctcctaac ccccaaccac tttacgcaaa atatcgatat
    11881 acaccggcat caacgggtgg cgaattaaca ccaccagcgc caccacgccg atggcagaaa
    11941 ttaacggcgt cagccacaac aaacgaccac gggacaaata atgactcaaa cggtcagtcg
    12001 ctttaccact gcgccataac cgccagcaca gccagccacc aacccacaaa aacaccgctg
    12061 ttgccagcag caaccattta aactcgccac tctgcattcc ggctggaata tcgatcgccg
    12121 cacccgccag aatccccggc aggaagtaaa acggcggcca cagcaggcag ccgataatat
    12181 tcggcgtaat aaattttgcc accgggagat ccagcattcc cgccaccatt ggcaccagcg
    12241 gacgcgtcgg gccaacaaaa cgaccgacca gaatggtgaa catgctgtgt tgatgcaacg
    12301 catgttcagt tttatcgagt aatgctttgt tcttcttcag aaatgaccag cgatgcaacg
    12361 gctttttaaa acgccagccc agccagaagg agatccagtc gcccagcagg caaccaacga
    12421 tccctgccag ccaggcgtgc cagaaactta actcgccgct gccaatcagc gctcccagcc
    12481 ccgccatcag caccgtaccg ggtagaatca aaccgaccag cgccagcgac tccagaaagg
    12541 ccaccaacac caccgccatc aatgaataca cggtggattg ggtaataaag tgttccagca
    12601 atgcttgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
    12661 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
    12721 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccagt gcatttttta
    12781 aatactcgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtagcgata
    12841 ggcatccggg tggtgctcaa aagcagcttc gcctgactaa tgcgttggtc ctcgcgccag
    12901 cttaagacgc taatccctaa ctgctggcgg aacagatgtg acagacgcga cggtgacaag
    12961 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
    13021 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
    13081 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
    13141 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
    13201 gcttcatccg ggcgaaagaa acccgtattg gcaaatattg acggccagtt aagccattca
    13261 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
    13321 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
    13381 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
    13441 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
    13501 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
    13561 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
    13621 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac ccaaccggta
    13681 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
    13741 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
    13801 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
    13861 tcgcaactct ctactgtttc tccatacccg tttttttgga tggagtgaaa cgatggcgat
    13921 tgcaattggc ctcgattttg gcagtgattc tgtgcgagct ttggcggtgg actgcgccag
    13981 cggtgaagag atcgccacca gcgtagagtg gtatccccgt tggcaaaaag ggcaattttg
    14041 tgatgccccg aataaccagt tccgtcatca tccgcgtgac tacattgagt caatggaagc
    14101 ggcactgaaa accgtgcttg cagagcttag cgtcgaacag cgcgcagctg tggtcgggat
    14161 tggcgttgac agtaccggct cgacgcccgc accgattgat gccgacggta acgtgctggc
    14221 gctgcgcccg gagtttgccg aaaacccaaa cgcgatgttc gtattgtgga aagaccacac
    14281 tgcggttgaa gaagcggaag agattacccg tttgtgccac gcgccgggca atgttgacta
    14341 ctcccgctac attggtggta tttattccag cgaatggttc tgggcaaaaa tcctgcatgt
    14401 gactcgccag gacagcgccg tggcgcaatc tgccgcatcg tggattgagc tgtgcgactg
    14461 ggtgccagct ctgctttccg gtaccacccg cccgcaggat attcgtcgcg gacgttgcag
    14521 cgccgggcat aaatctctgt ggcacgaaag ctggggcggc ttgccgccag ccagtttctt
    14581 tgatgagctg gacccgatcc tcaatcgcca tttgccttcc ccgctgttca ctgacacctg
    14641 gactgccgat attccggtgg gcaccttatg cccggaatgg gcgcagcgtc tcggcctgcc
    14701 tgaaagcgtg gtgatttccg gcggcgcgtt tgactgccat atgggcgcag ttggcgcagg
    14761 cgcacagcct aacgcactgg taaaagttat cggtacttcc acctgcgaca ttctgattgc
    14821 cgacaaacag agcgttggcg agcgggcagt taaaggtatt tgcggtcagg ttgatggcag
    14881 cgtggtgcct ggatttatcg gtctggaagc aggccaatcg gcgtttggtg atatctacgc
    14941 ctggttcggt cgcgtactca gctggccgct ggaacagctt gccgcccagc atccggaact
    15001 gaaagcgcaa atcaacgcca gccagaaaca actgcttccg gcgctgaccg aagcatgggc
    15061 caaaaatccg tctctggatc acctgccggt ggtgctcgac tggtttaacg gtcgtcgctc
    15121 gccaaacgct aaccaacgcc tgaaaggggt gattaccgat cttaacctcg ctaccgacgc
    15181 tccgctgctg ttcggcggtt tgattgctgc caccgccttt ggcgcacgcg caatcatgga
    15241 gtgctttacc gatcagggga tcgccgtcaa taacgtgatg gcgctgggcg gcatcgcgcg
    15301 gaaaaaccag gtcattatgc aggcctgctg cgacgtgctg aatcgcccgc tgcaaattgt
    15361 tgcctctgac cagtgctgtg cgctcggtgc ggcgattttt gctgccgtcg ccgcgaaagt
    15421 gcacgcagac atcccatcag cccagcaaaa aatggccagt gcggtagaga aaaccctgca
    15481 accgcgcagc gaacaggcac aacgctttga acagctttat cgccgctatc agcaatgggc
    15541 gatgagcgcc gaacaacact atcttccaac ttccgccccg gcacaggctg cccaggccgt
    15601 tgcgactcta taaggacacg ataatgacga tttttgataa ttatgaagtg tggtttgtca
    15661 ttggcagcca gcatctgtat ggcccggaaa ccctgcgtca ggtcacccaa catgccgagc
    15721 acgttgttaa tgcgctgaat acggaagcga aactgccctg caaactggtg ttgaaaccgc
    15781 tgggcaccac gccggatgaa atcaccgcta tttgccgcga cgcgaattac gacgatcgtt
    15841 gcgctggtct ggtggtgtgg ctgcacacct tctccccggc caaaatgtgg atcaacggcc
    15901 tgaccatgct caacaaaccg ttgctgcaat tccacaccca gttcaacgcg gcgctgccgt
    15961 gggacagtat cgatatggac tttatgaacc tgaaccagac tgcacatggc ggtcgcgagt
    16021 tcggcttcat tggcgcgcgt atgcgtcagc aacatgccgt cgttaccggt cactggcagg
    16081 ataaacaagc ccatgagcgt atcggctcct ggatgcgtca ggcggtttct aaacaggata
    16141 cccgtcatct gaaagtctgc cgttttggcg ataacatgcg tgaagtggcg gtcaccgatg
    16201 gtgataaagt tgccgcacag atcaagttcg gtttctccgt caatacctgg gcggttggcg
    16261 atctggtgca ggtggtgaac tccatcagcg acggcgatgt taacgcgctg gtcgatgagt
    16321 acgaaagctg ctacaccatg acgcctgcaa cacaaatcca cggcgaaaaa cgacagaacg
    16381 tgctggaagc ggcgcgtatt gagctgggga tgaagcgttt cctggaacaa ggtggcttcc
    16441 atgcgttcac caccaccttt gaagatttgc acggtctgaa acagcttcct ggtctggccg
    16501 tacagcgtct gatgcagcag ggttacggct ttgcgggcga aggcgactgg aaaactgccg
    16561 ccctgcttcg catcatgaag gtgatgtcaa ccggtctgca gggcggtacc tcctttatgg
    16621 aggactacac ctatcacttc gagaaaggca atgacctggt gctcggctcc catatgctgg
    16681 aagtctgccc gtcgattgcc gtagaagaga aaccgatcct cgacgttcag catctcggta
    16741 ttggtggtaa ggacgatcct gcccgcctga tcttcaatac ccaaaccggc ccagcgattg
    16801 tcgccagtct gattgatctc ggcgatcgtt accgtctgct ggttaactgc atcgacacgg
    16861 tgaaaacacc gcacaccctg ccgaaactgc cggtggcgaa tgcgctgtgg aaagcgcaac
    16921 cggatctgcc aactgcttcc gaagcgtgga tcctcgctgg tggcgcgcac cataccgttt
    16981 tcagccatgc gctgaacctc aacgatatgc gccagttcgc cgagatgcac gacattgaaa
    17041 tcacggtaat tgataacgac acccgcctgc cagcgtttaa agacgcgctg cgctggaacg
    17101 aagtgtatta cggatttcgt cgctaagtag ccgcatcagg tgtgtaacgc ctgatgcgac
    17161 gctgacgcgt cttatcaggc ctacacgctg cgattttgta ggccggataa gcaaagcgca
    17221 tccggcattc aacgcctgat gcgacgctgg cgcgtcttat caggcctacg cgttccgatt
    17281 ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac gctggcgcgt
    17341 cttatcaggc ctacacgctg cgattttgta ggccggataa gcaaagcgca tccggcacga
    17401 aggagtcaac atgttagaag atctcaaacg ccaggtatta gaggccaacc tggcgctgcc
    17461 aaaacataac ctggtcacgc tcacatgggg caacgtcagc gccgttgatc gcgagcgcgg
    17521 cgtctttgtg atcaaacctt ccggcgtcga ttacagcatc atgaccgctg acgatatggt
    17581 cgtggttagc atcgaaaccg gtgaagtggt tgaaggtgcg aaaaagccct cctccgatac
    17641 gccaactcac cgactgctct atcaggcatt cccgtccatt ggcggcattg tgcacacaca
    17701 ctcgcgccac gccactatct gggcgcaggc gggccagtcg attccagcaa ccggcaccac
    17761 ccacgccgac tatttctacg gcaccattcc ctgtacccga aaaatgaccg acgcagaaat
    17821 caacggcgaa tatgagtggg aaaccggtaa cgtcatcgtc gaaaccttcg aaaaacaggg
    17881 tatcgatgca gcgcaaatgc ctggcgtgct ggtccattct cacggcccat ttgcatgggg
    17941 caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg cttatatggg
    18001 gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc tgctggataa
    18061 acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac tgtataaaac
    18121 cacagccagt caaattaaac caggctataa tcaagcctgg ttttttgatg gattttcagc
    18181 gtggcgcagg caggttttat cttaacccga cactggcggg ataccccgca aggaacagag
    18241 gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttttccttgc gccgcaagag
    18301 tccgtggcgt ttattcccgc cgatcaggtt ccccgcgctc agcatatttt gcggggtgaa
    18361 caaggctttc gcctgacgcc gctggcgtta aaggattttc accgccagcc ggtgtatggc
    18421 ctttactgtc gcgcccatcg ccagttgatg aattacgaaa agcgcctgcg tgaaggtggc
    18481 gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga gcggtttatc
    18541 acctcaccgg tgtgggtcga gggtgatatg tacaatggcg ctatcgttaa tgcccgtctg
    18601 aaaccgcatc ccgactatcg tccaccgctc aagtgggttt ctattgatat tgaaaccacc
    18661 cgccacggcg agctgtactg catcggcctg gaaggctgcg ggcagcgcat cgtttatatg
    18721 ctggggccgg agaatggcga cgcctccgcg ctcgattttg aactggaata cgtcgccagc
    18781 cgcccgcagc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc cgatgtgatc
    18841 atcggctgga acgtggtgca attcgatcta cgaatgctgc aaaaacatgc cgagcgttat
    18901 cgtattccgc tgcggctggg gcgcgataac agcgagctgg agtggcgcga gcacggcttt
    18961 aaaaacggcg tcttttttgc ccagtctaaa gggcggctaa ttatcgacgg tatcgaggcg
    19021 ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc tcaggagcta
    19081 ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat tgaccgccgt
    19141 ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga gctggtgacg
    19201 cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac ggtgaacggc
    19261 ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta ttttccccgt
    19321 atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca cgccagccct
    19381 ggcggctacg tgatggattc gcgacctggg ctttatgatt cggtgctggt gctggactac
    19441 aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg gctggtggaa
    19501 ggcatggcgc agcctgatcc agagcacagc accgaaggct ttctcgatgc ctggttctcg
    19561 cgagaaaaac attgcctgcc ggagattgtg actaacatct ggcacggacg cgatgaagcc
    19621 aaacgccagg gtaataagcc gctgtcgcag gcgctgaaaa tcatcatgaa tgccttttat
    19681 ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc gtcgatcacc
    19741 atgcgtggtc atcagatcat gcggcaaacc aaagcgttga ttgaagcgca gggctacgac
    19801 gtgatctacg gcgataccga ctcaacgttt gtctggctga aaggcgcaca ttcggaagaa
    19861 gaagcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg ggcggaaacg
    19921 ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaaccca tttctgccgt
    19981 tttctgatgc caaccattcg cggagccgat accggcagta aaaagcgtta tgccggactg
    20041 attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt gcgtaccgac
    20101 tggacgccgc tggcccagca gtttcagcag gagctatacc tgcgtatctt ccgtaacgag
    20161 ccatatcagg agtacgtccg cgaaaccatc gacaaactga tggcgggtga actggatgcg
    20221 cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg taatgttccg
    20281 cctcatgtac gcgccgctcg ccttgccgat gaagaaaacc aaaagcgcgg tcgccccttg
    20341 caatatcaga accgcggcac cattaagtac gtatggacca ccaacggccc ggagccgctg
    20401 gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct acaacccgtg
    20461 gcggagggaa tacttccttt tattgaggat aattttgcta cacttatgac cgggcaactt
    20521 gggctatttt gagcaaaaaa agagttcgcc agataccatt ttgatgcgtg acgaatgctt
    20581 tgccatccag taccatagcg ccctttccat tcctggacct gaataacacc actacctcat
    20641 aagcacggta gcgggtggtt attgcctgca attaaagata tagagccgaa cacatatgcc
    20701 ttttacactt ggtcaacgct ggatcagcga tacagaaagc gaattgggac ttggaaccgt
    20761 tgtcgcggtg gatgcgcgaa ctgtcacttt acttttccca tctactggtg aaaaccgtct
    20821 gtacgcacgc agtgattccc ccgtgacccg cgtgatgttc aaccctggtg ataccattac
    20881 cagccatgac ggctggcaga tgcaagtcga agaagtaaaa gaagaaaatg gcttgctgac
    20941 ctatatcggt actcgcctgg atactgaaga gtccggcgta gccctgcgtg aagttttcct
    21001 tgatagcaaa ctggtgttca gcaaaccgca ggaccgcctg tttgccgggc agattgaccg
    21061 tatggaccgc tttgcgctgc gttatcgcgc gcgtaagtat tccagcgaac agttccgtat
    21121 gccgtacagc ggcctgcgcg gtcagcgtac cagcctgatc ccgcaccagc tcaacatcgc
    21181 tcatgatgtt ggccgccgcc acgcgccgcg cgtcctgctg gctgacgaag tgggtttagg
    21241 gaaaaccatt gaagccggga tgatccttca tcagcaactg ctctctggcg ctgctgaacg
    21301 tgtgctgatt atcgtcccgg aaaccttaca gcatcagtgg ctggtagaaa tgctgcgccg
    21361 tttcaacctg cgctttgcgc tgtttgatga tgagcgttat gccgaagctc agcacgatgc
    21421 ttacaacccg ttcgacaccg aacagctggt gatttgctcg ctggattttg cccgtcgtag
    21481 caaacagcgt ctggaacatc tctgtgaagc cgagtgggac ctgctggtgg tcgatgaagc
    21541 gcatcacctg gtgtggagcg aagatgcgcc aagccgtgaa tatcaggcca ttgaacaact
    21601 ggcagagcac gtgccgggcg ttctgctgct gaccgcgacc ccggaacagc tggggatgga
    21661 aagccacttc gcccgtctgc gtctgctgga cccgaaccgt ttccacgatt ttgcgcagtt
    21721 cgttgaagag cagaaaaatt atcgtccggt tgcggacgcc gttgccatgc tgctggcagg
    21781 taacaaactg agcaatgacg aactgaacat gctcggcgag atgatcggcg agcaggatat
    21841 cgagccgctg ttgcaagcag caaacagcga cagcgaagat gcccagagcg cccgtcagga
    21901 actggtttcg atgctgatgg atcgccacgg caccagccgc gtgctgttcc gtaacacccg
    21961 taacggtgtg aaaggattcc cgaaacgcga gctgcacacc attaagctgc cgctaccgac
    22021 gcagtatcag acggctatta aagtctccgg cattatgggc gcacgtaaaa gtgcggaaga
    22081 tcgtgctcgc gatatgctct acccggagcg tatttatcag gaatttgaag gtgataacgc
    22141 cacctggtgg aacttcgatc cgcgcgttga gtggctgatg ggctacctga ccagccatcg
    22201 ctctcagaaa gtgctggtga tctgcgctaa agctgccact gcgctgcaac tggagcaagt
    22261 actgcgcgaa cgtgaaggta tccgcgctgc tgtgttccac gaaggcatgt cgattattga
    22321 gcgtgaccgc gctgccgcct ggtttgctga agaagacacc ggcgcacagg tactgctgtg
    22381 ctcggaaatc ggttctgaag gacgtaactt ccagttcgcc agccacatgg tgatgtttga
    22441 cctgccattc aacccggatc tactggagca gcgtattggt cgtctggatc gtatcggcca
    22501 ggcgcacgat attcagatcc atgtgcctta tctggagaaa accgctcagt cggtgctggt
    22561 gcgctggtat cacgaaggtc tggatgcatt tgagcacacc tgcccgaccg gacgcactat
    22621 ttacgatagc gtatacaacg atctgattaa ctatctggct tcaccggatc aaaccgaagg
    22681 ctttgacgat ctgatcaaaa actgccgcga gcaacatgaa gcgctgaaag cacagctgga
    22741 acagggtcgt gaccgcctgc tggaaatcca ctccaacggt ggcgaaaaag cccaggcact
    22801 ggcagaaagc attgaagagc aggatgacga taccaacctg atcgccttcg ccatgaacct
    22861 gttcgatatt atcggtatca atcaggacga tcgcggcgac aacatgatcg tgctgacgcc
    22921 gtccgatcat atgctggtgc cggacttccc tggcctgtcg gaagatggca tcaccatcac
    22981 ctttgatcgt gaagtggcgc tggcgcgtga agatgcacag tttattacct gggagcatcc
    23041 gctgatccgc aacggtctgg atctgatcct ttctggcgat accggtagca gcacgatttc
    23101 actgttaaaa aacaaagcgt tgccggtagg tacgctgttg gtggaactga tttatgtggt
    23161 tgaagcccag gctccgaagc agttgcagct caaccgcttc ctgccaccga cgccggtacg
    23221 tatgctgctg gataaaaacg gcaacaacct ggcggcgcag gtagagtttg aaacctttaa
    23281 ccgccagctt aacgcggtta accgtcacac cggcagcaaa ctggttaacg ccgtgcagca
    23341 ggatgttcac gctatccttc aactgggtga agcgcagatc gagaaatctg cccgtgcatt
    23401 gattgatgca gcgcgtaacg aagccgacga aaaactgtct gccgagctgt ctcgtctgga
    23461 agctctgcgt gcagtgaacc cgaacattcg tgacgacgaa ctgaccgcca ttgagagcaa
    23521 ccgtcagcag gtaatggaaa gcctggatca ggcaggttgg cgtctggatg ccctgcgttt
    23581 gatcgttgta acgcatcagt aacggagccg aaaatgggga tggaaaacta caatccaccg
    23641 caggaaccct ggttggttat cctgtatcag gatgaccata ttatggtggt caacaagccg
    23701 agcggtttgt tgtcagtgcc gggtcgtctg gaagagcaca aagacagcgt gatgacgcgc
    23761 attcagcgtg attatccgca ggcagaatcg gtgcatcgtc tggatatggc taccagcggc
    23821 gtgattgtag tggcgctgac caaagccgcg gagcgggagt taaaacgcca gttccgcgag
    23881 cgcgagccga aaaagcagta tgtggcccgc gtctgggggc atccatcccc cgcagaaggt
    23941 ctggtggatc tgccgctgat ttgcgactgg ccaaaccgcc cgaaacagaa agtctgttac
    24001 gaaacgggta aacctgcgca gacggaatat gaagtggtgg agtatgcggc ggataacacg
    24061 gcaagagtgg tgttaaaacc gattaccggg cgttcgcatc aattgcgtgt gcatatgctg
    24121 gcgctggggc atccgattct cggcgatcgt ttttatgcat caccagaagc gagagcgatg
    24181 gcaccgcgtt tgttgctaca tgcagagatg ttgacgatta cccatccggc gtatggcaat
    24241 agtatgacgt ttaaagcgcc agcggatttt taatcgtcac gctggcgcag ttcggtaggc
    24301 ggataaggcg ttcacgccgc atccggcaat tgtgcgccga ttgcctgatg cgacgcggcc
    24361 gcgtcttatc aggcctacag tttaaaactt atttaaaccc tttctgctgc tttatcagct
    24421 catatgcctg ctgaatttcc tgcgctttct gcttcgccat ctccatcatc tcaggcggca
    24481 aacctttcgc caccagctta tcgggatggt gttcactcat cagcttacgg taggcacgtt
    24541 tgatggtggt cgcatcatcc gtcggcttca cgcccagcac attacaggca tcttccagcg
    24601 tcgggccacg ctgcgcttgc tgccagttac caccgccaga ttgctgctga taaccgccgc
    24661 caaactgtgc accgccctgc atcatgcgca aaaactggtc aaactgggcg cgggagatcc
    24721 ctaattcttc agcaatgaca tacagcaccg cccgttcatt cgggtgcagt gaaccatcag
    24781 caaacgccgc ctgaatctgg atctccagaa acatacgaat taagtcaaaa cgaccaaagc
    24841 agacactgcg aaactggcgc atcttttcgc gcagcgggta attgtctgat tttcccaccc
    24901 ggaacgcatt ttgcgccgca gtacgggaag cgccatgaag attcattcgg tccatcaact
    24961 ggctggcgat atgaatatca gcctccgtga cgcgaccttt ggatttggtt aaatgcccca
    25021 tcacttcaaa agtggtggca aaaaacagcg cctgacgctc acgctggttg gcgaaccacg
    25081 ccattttacg gctacgggct ttatcaaaca tatggccaat taacaggcct aacactacgc
    25141 cccaaaagcc gccgcccatc agtaaggcca cggccacgcc aatgattttt ccccaatact
    25201 gcatatattc cccaaatcga cacacggata tcagggctat ctcccacaat ataaaggtgc
    25261 ttttaccgtt ttccggctgc ggtcaattgt gggacatcgc ctataatttg cattatcata
    25321 cctgtcattc aatgccgtgc ctaacaccac tgacgctatt cggacaggat taacactagc
    25381 gtagagatga caagtacgtt agtctctgac cgtttgtcac gcgcaacgtt accgatgatg
    25441 gaacaataaa atcaacgtat gaaaaaacgt atccccactc tcctggccac catgattgcc
    25501 accgcccttt atagtcaaca gggactggca gccgacctcg cctcacagtg catgttgggc
    25561 gtgccaagct atgaccgtcc tctggtacag ggcgatacca atgacttacc cgtgactatc
    25621 aatgctgacc acgcgaaagg ggactacccg gatgacgccg tgtttactgg cagcgtggat
    25681 atcatgcagg gtaacagccg tctgcaggcc gacgaagtgc agctccatca aaaagaggca
    25741 ccaggacaac cggagccggt acgtaccgtt gatgcgctcg gtaatgtcca ttacgacgat
    25801 aaccaggtga tcctcaaagg gccgaaaggc tgggcgaatc tgaacaccaa agataccaac
    25861 gtctgggaag gtgattacca gatggtgggt cgccagggtc gcggtaaagc ggacctgatg
    25921 aaacaacgtg gcgaaaaccg ctataccatt ctggataacg gtagctttac ctcctgtctg
    25981 ccgggttctg acacctggag cgtggtaggt agcgaaatta ttcatgaccg cgaagaacaa
    26041 gttgcggaga tctggaacgc ccgctttaag gtgggtccgg taccgatctt ttatagcccc
    26101 tatttgcagt tgccggtggg tgacaaacgt cgctctggtt tcttgatccc gaacgccaag
    26161 tacaccacca ccaactactt tgagttctac cttccatatt actggaacat cgcgccaaat
    26221 atggatgcca ccatcacgcc gcattatatg catcgtcgtg gcaacatcat gtgggagaac
    26281 gaattccgct acctctccca ggcgggcgct ggcttgatgg aactggacta tctgccttca
    26341 gataaagtct atgaagatga acacccgaac gatgatagtt cacgtcgttg gttattctac
    26401 tggaaccact ccggggtcat ggatcaggtg tggcgtttta acgtcgacta caccaaggtc
    26461 agcgatccta gctacttcaa tgatttcgat aacaagtacg gttccagtac tgacggctac
    26521 gcaacgcaaa aattcagcgt tggctatgcg gtgcaaaact tcaatgccac cgtttcaacc
    26581 aagcagttcc aggtcttcag cgaacagaac accagtagct actcggcaga gccgcagtta
    26641 gacgttaact actaccagaa tgatgttggt ccgtttgata cacgcattta cggccaggca
    26701 gtgcactttg taaacaccag agacgacatg cctgaagcaa cccgtgttca cctggagccg
    26761 accatcaatt tgccgctctc taataactgg ggcagcatca ataccgaagc gaagttgctg
    26821 gcaacccatt atcagcaaac taatcttgac tggtataaca gcaatccaca aaacaataag
    26881 ttggcagatt ctgttaaccg tgtaatgccg caatttaaag ttgacggtaa aatggtcttc
    26941 gaacgtgata tgaatctact ggcaccgggc tttacccaaa cgctggaacc gcgcgcgcag
    27001 tatttgtacg tgccgtatcg cgatcagagc gatatctaca actacgactc tgccttattg
    27061 caatccgatt attcgggctt gttccgggac cggacttacg gcggtcttga ccgtattgcc
    27121 tccgctaacc aggtgacgac cggtgtcaca tctcgcgtat atgatgatgc tgccgttgaa
    27181 cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac tggcgatgac
    27241 aacataacat gggagaatga cgacaaaacg ggatcactgg tgtgggcagg cgacacctac
    27301 tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgacac tcgcctggat
    27361 aacgtggcga ccagtagttc tagtattgag tatcgccgag atgaagaccg tctggtgcag
    27421 ctgaactacc gttacgccag cccggaatat attcaggcta cactgccaag agacagtaga
    27481 gataaacgtt ggaatgcccc tcagtacaaa gatggtattt cgcaggtagg tgccgtcgcc
    27541 agctggccaa ttgccgatcg ttggtcaatt gttggggcct actactacga caccaatgcg
    27601 aacaagcaag ccgactctat gttaggtgtg caatacagct cctgctgcta tgcaattcgc
    27661 gtcggttacg agcggaagct gaacggttgg gataacgata aacaacatgc ggtatatgac
    27721 aacgcaatcg gctttaacat cgaacttcgc ggcctgagct ccaactacgg cctgggtacg
    27781 caagagatgc tgcgttcgaa cattctgccg tatcaaaaca ctttgtgatt tgttgattta
    27841 ccacgtaatc cgcagtgcgg ttaattgaaa tggaaaaagt atgaagaact ggaaaacgct
    27901 gcttctcggt atcgccatga tcgcgaatac cagtttcgct gccccccagg tagtcgataa
    27961 agtcgcagcc gtcgtcaata acggcgtcgt gctggaaagc gacgttgatg gattaatgca
    28021 gtcggtaaaa ctgaacgctg cgcaggcaag gcagcagctt cctgatgacg cgacgctgcg
    28081 ccaccaaatc atggaacgtt tgatcatgga tcaaatcatc ctgcagatgg ggcagaaaat
    28141 gggagtgaaa atctccgatg agcagttgga tcaggcgatt gctaacatcg cgaaacagaa
    28201 caacatgacg ctggatcaga tgcgcagccg tctggcttac gatggtctga actacaacac
    28261 ctatcgtaac cagatccgca aagagatgat tatctctgaa gtgcgtaaca acgaggtgcg
    28321 tcgtcgcatc accatcctgc cgcaggaagt cgaatccctg gcgcagcagg tgggtaacca
    28381 aaacgacgcc agcactgagc tgaacctgag ccacatcctg atcccgctgc cggaaaaccc
    28441 gacttctgat caggtgaacg aagcggaaag ccaggcgcgc accattgtcg atcaggcgcg
    28501 taacggcgct gatttcggta agctggcgat tgctcattct gccgaccagc aggcgctgaa
    28561 cggcggccag atgggctggg gccgtattca ggagttgccg ggtatcttcg cccaggcatt
    28621 aagcaccgcg aagaaaggcg acattgttgg cccgattcgt tccggcgttg gcttccatat
    28681 tctgaaagtt aacgacctgc gcggcgaaag caaaaatatc tcggtgaccg aagttcatgc
    28741 tcgccatatt ctgctgaaac cgtcgccgat catgactgac gaacaggccc gtgtgaaact
    28801 ggaacagatt gctgccgata tcaagagtgg taaaacgact tttgctgccg cagcgaaaga
    28861 gttctctcag gatccaggct ctgctaacca gggcggtgat ctcggctggg ctacaccaga
    28921 tattttcgat ccggccttcc gtgacgccct gacccgcctg aacaaaggtc aaatgagtgc
    28981 accggttcac tcttcattcg gctggcattt aatcgaactg ctggataccc gtaatgtcga
    29041 taaaaccgac gctgcgcaga aggatcgtgc ataccgcatg ctgatgaacc gtaagttctc
    29101 ggaagaagca gcaagctgga tgcaggaaca acgcgccagc gcctacgtta aaatcctgag
    29161 caactaatgg ttaaaaccca acgtgttgtg atcactcccg gcgagcccgc cgggattggc
    29221 ccggacttag ttgtccagct tgcacagcgt gagtggccgg tcgaactggt tgtttgtgcc
    29281 gatgccactc tccttaccga ccgggcagcg atgctcggtt tgccgctcac cctccgccct
    29341 tattccccca actcccctgc acaaccgcaa actgcgggca cattaacgct acttcctgtc
    29401 gcgctacgcg aatctgtcac tgcggggcag ttagcgattg aaaatgggca ttacgtggtg
    29461 gagacgctgg cgcgagcgtg tgatggctgt ctgaacggtg aatttgctgc gctgatcaca
    29521 ggccccgtgc ataaaggcgt cattaacgac gcaggtattc cgtttactgg tcataccgag
    29581 tttttcgaag agcgctcgca ggcgaaaaaa gtggtgatga tgctggcgac cgaagaactt
    29641 cgcgtggcgc tggcaacgac gcatttaccg ctgcgcgata tcgcagatgc tatcacccct
    29701 gcacttttgc acgaagtgat tgctattttg catcacgatt tgcgtacaaa atttggtatt
    29761 gccgaaccgc gcattctggt ctgcgggctg aatccgcacg cgggcgaagg cggtcatatg
    29821 ggtacggaag agatagacac cattattccg gtgctcgacg agctgcgggc gcaggggatg
    29881 aaactcaacg ggccgctgcc tgccgatacc ctgtttcagc cgaaatatct cgataacgcc
    29941 gacgccgtgc tggcgatgta ccacgatcag ggtcttcccg tgctaaaata ccagggcttc
    30001 gggcgcggtg tgaacattac gctgggcctg ccctttattc gcacatcagt ggaccacggc
    30061 accgcgcttg aactggcggg acgtggcgaa gccgatgtcg gcagttttat tacggcgctt
    30121 aatttcgcca tcaaaatgat tgttaacacc caatgaataa tcgagtccac cagggccact
    30181 tagcccgtaa acgcttcggg caaaactttc tcaacgatca gttcgtgatc gacagcattg
    30241 tttctgccat taacccgcaa aagggccagg caatggtcga aatcggcccc ggtctggcgg
    30301 cattgaccga gccggtcggc gaacgtctgg accagctgac ggtcatcgaa cttgaccgcg
    30361 atctggcggc acgtctgcaa acgcatccat tcttaggccc gaaactgacg atttatcagc
    30421 aggatgcgat gacctttaac tttggcgaac tggccgagaa aatgggtcag ccgctgcgtg
    30481 ttttcggcaa cctgccttat aacatctcca cgccgttgat gttccatctg tttagctata
    30541 ctgatgccat tgccgacatg cactttatgt tgcaaaaaga ggtggtgaat cgtctggttg
    30601 caggaccgaa cagcaaagcg tatggtcgat taagcgtcat ggcgcaatac tattgcaacg
    30661 tgatcccggt gctggaagta ccgccgtcag cctttacacc accacccaaa gtggattccg
    30721 ccgtcgtgcg cctggttcct catgcaacga tgcctcaccc ggttaaagat gttcgcgtgt
    30781 tgagccgcat caccactgaa gcctttaacc agcgtcgtaa aaccattcgt aacagcctcg
    30841 gcaacctgtt tagcgtcgag gtgttaacgg gaatggggat cgacccggcg atgcgagcgg
    30901 aaaatatctc tgtcgcgcaa tattgccaga tggcgaacta tctggcggag aacgcgcctt
    30961 tgcaggagag ttaacgatga tcaattcgcc ccgagtgtgt attcaggttc aaagcgtcta
    31021 cattgaggca caatcttcac ctgataatga acgttacgtt tttgcttata ccgtaaccat
    31081 acgcaatctg gggcgagcgc cagtgcagtt gttggggcgt tactggctga tcaccaatgg
    31141 caatggccgt gaaaccgaag tccagggcga aggagtggtt ggcgtccagc cacttatcgc
    31201 gcctggcgaa gagtaccagt acaccagcgg tgcaatcatt gaaaccccgc tgggcaccat
    31261 gcagggtcac tacgaaatga tcgatgaaaa tggcgtccct ttcagcatcg acattcccgt
    31321 attccgactc gccgttccca cactcattca ttaaaagaat atggcgacat accttattgg
    31381 cgacgttcat ggttgttacg atgaactgat cgcattgctg cataaagtag aatttacccc
    31441 tgggaaagac accctctggc tgacaggcga tctggtcgcg cgcggccctg gttcgctgga
    31501 tgttctgcgc tatgtgaaat ccttaggcga cagcgtacgt ctggtgctgg gcaatcacga
    31561 tctgcatctg ctggcggtat tcgccgggat cagccgcaat aaaccgaaag atcgcctgac
    31621 accgctgctg gaagcaccgg atgccgacga gctgcttaac tggctgcgtc gccagcctct
    31681 gctgcaaatc gacgaagaga aaaagttggt gatggctcac gccgggatca cgccgcagtg
    31741 ggatctgcag accgccaaag agtgcgcgcg cgatgtagaa gcggtgctgt cgagcgactc
    31801 ctaccccttc ttccttgatg ccatgtacgg cgatatgcca aataactggt caccggaatt
    31861 gcgggggctg ggaagactgc ggtttatcac caatgcattt acccggatgc gtttttgctt
    31921 cccgaacggg caactggata tgtacagcaa agaatcgccg gaagaggccc ctgccccgtt
    31981 gaaaccgtgg tttgcgattc ctggccctgt cgctgaagag tacagcatcg cctttggtca
    32041 ctgggcatcg ctggaaggca aaggtacgcc ggaaggtatt tacgcgctgg ataccggctg
    32101 ctgctggggg ggtacattaa cctgcctgcg ctgggaagat aaacagtatt ttgtccagcc
    32161 gtcgaaccgg cataaggatt tgggcgaagg agaggcggtc gcatcttaac gcattaccat
    32221 tcagggagga ataatggctg actattttga aatacgtgct gattacaatc agcacaccat
    32281 cacggtttat caggcttaca atgatgcaat cgctgacgtc gccgtacgtg acgggcgatt
    32341 tgacgcacct ttttcattca accgcatgac ctggattaaa ccctctttta tgtggatgat
    32401 ggagcgtagc aactggggac tgaaaaaaga tcaacaacgc attctcgcaa tccgaatcaa
    32461 acgcaccttc tttgatacat tgctggaaca agcggagcta accacgccgg aagcccatgt
    32521 ctacccacat gcaggagttt gggaaacgtt atttgcgcaa gcaaatgtat atgttcagtg
    32581 ggacccagaa cgcagcataa atggcaaaaa actggagcac cgatcattac agcttggtat
    32641 ttctcgtaac ttgatcagtc aatttaacga agacgcgatt gttgccattg atgatctgac
    32701 gccgctggtg cgaaagtgtc ataacctatt gataaacggc aaaacaacac aggcgaaatc
    32761 cttcctgccg ccagaaaaaa tttaccctgt tagtgcagct gcgagaaagg ctttgggaat
    32821 gaaaaattcc tgagacaaat atgcgcctat taagcggaaa atatctaagt gcgacaaatc
    32881 aacgccgggc ttaataccag agaaattatt ctggaaatga ttgacgaagc agatgaaccc
    32941 ccatttgctg agcagcgaac ctggcatcgc aagcgaatcg cttttctatg tgattgcatc
    33001 agcgttgctg tcgactacgg cttcttctgt tctgtctctc agcagtcaat aatgaacata
    33061 accaggatgg ctgaagatgt atggataaca gaaagcaaaa cgacgcaaat cattgatatc
    33121 tggcaacaaa ttaagcggct ttatccgcca aacattattc cctgcaccca gcagttaccc
    33181 gctaaagaat tgcttgaact gctactttcc agtcctgacg atcttcgaat aattgaaatc
    33241 cctgacatca tcggtgatgt ttttgatgac caaacctgga ttcgattatt caaaaaacac
    33301 tttgctgata ttatcttact acgatgtaat gtgtaacaat ctaaaataca tcgccagacg
    33361 acagaccaaa aagaaagtat ttagcagata ctcaacattc tgacttctgt acagaaatag
    33421 cagagtttaa aaacttaaaa tttccggtac tcatcagaga aacatcctgt ttcttctgca
    33481 agttcattta atgccgagat ggcatcctcg tttacttcag tccactgtag agtggcttga
    33541 gagtttagac ctacgcaatg agcgcgactc acaaaatcgc gctcactggt aaaatcagac
    33601 gtcttatcgt tcaatttgta gtcatagcaa aaacttaccg ccgctccaga atctcaaagc
    33661 aatagctatg agagttctgc gcatcagcgt cgtggaattc gctgaatacc gattcccagt
    33721 catccggctc gtaatccggg aaatgggtgt cgccttccac ttctgcgtcg atatgcgtca
    33781 gatacagttt ttgcgctttg ggcaggaact gctcataaac gcgaccaccg ccaatcacca
    33841 tgatttctgg tacgtcacca cacgccgcga tggcttcatc caccgacttc acccacgtta
    33901 cgcgatcatc cgtacccggt tgactgctga ggataatatt tttgcgtcct ggcagcggac
    33961 gaccgatgga ttcccaggta tggcggccca taatcacggg tttatttaag gtgttgcgtt
    34021 taaaccaggc gagatcggca ggcaggttcc acggcatggc gttttccatg ccgataacgc
    34081 gatctaccgc taacgccgca atcagactga tcattgagat ttcccgataa aaaaaattgt
    34141 cgccactata cgtaaagcgt aaaccgtcgt cgactggtgc gaggatgatg ttgaggaaaa
    34201 ttttatattc tgctggcgag tccacgctct ctccctggac tcgccgcatt acaatgaaac
    34261 aaaaacaaac agttagctgt aaagtgtgat ttacgtcact ctttattagg atgagggttt
    34321 cgtttccggt tcatccgcca tgttgccggt atgtttacct tcttcggttc cctgccagcc
    34381 atgtcgttga attaatgaca gatgttcacg gtcctcggtg atgatctcac ttaacatcgc
    34441 gctggtgcgt ttatagaccg ccgcgcgggc tttggtgtcg ttctcaacca ttgccatctc
    34501 ttccaccatc tgaatattaa aacggcgaaa aacatcggcg cgctcccgtg cttcatacgg
    34561 ccccagacct aaactttcca gcgccagacg cccggttttg agcgcccctt caaaggtttc
    34621 acgctctggc ttttcaacgc ctgcctgacg caaacgaatg tagtggtcga catcgcgggc
    34681 gcgggcaata atctgcaaat gcgggaaatg ttctttcacc atctctgtca gttgcaggtt
    34741 ggtttgcgga tcgtcgatgg cgttaatcag cacttccgct ttcgccgctc ccgcagattc
    34801 cagtaaatcc atccgcgtgg catcgccgta aaacactttc atgccgaatt tacgcagcgt
    34861 ttcgatatgg tccggatcgt gatcgagtac caccattttc accccgctgg agagcagtaa
    34921 acgtccggtg atctgcccga aacgaccgaa tccggcgata atcacgcgcg gctgttcttc
    34981 atcgatctca tcggcttcac gcgcctcttc agtagaagat tgctcaaggc gattgaggat
    35041 caccagcaga atcggcgttg ccgccatcga cagcgccacc gccagggtca gcgatttcgc
    35101 ccactccggc tccagcacat tcgccatttg cgccgcacca aataccacaa aggcaaactc
    35161 actgccctgc cctaacagca ccgcaaacca gcgacgctgt ttatttggca cctgcaacgg
    35221 tcgggcaatc agccacagca tggcgatttt gatgatgagg aaaccgagca gcaaaatgac
    35281 aatgcgcaat gggttttcaa tcagcgtgcc aaagtctatc gacatgccaa caccgatgaa
    35341 aaacagcccc aacagcaaac ctttaaatgg ctcgatatcg ctctccagcg catgacggta
    35401 ttccgagctt gccagcagta cgcccgccag aaacgcgccc atcgccatcg acaagccaac
    35461 ctcttccagc agcaaaccaa agccaaacac gaggaataaa gctacggcac taaacacttc
    35521 ccgcaagcca gagcgggcaa caaaacgcag cgccggacgc gtgacatagc gccccagcaa
    35581 taccaccagc accagcgcgc ccgccacttt taacgccgag agagcaaatg cgcccatcgt
    35641 cgtcgaggca ctgctcgccg ccagtagcgg gatcatcgcc accagaggga tcgcagcgat
    35701 atcctggaac aacagcaccg caaaggcgct acggcccatc tgtgttacca tcaggttacg
    35761 ctcattcatc gcctgcatgg caatcgccgt agaggagagc gccagcgtca tgccgatcag
    35821 ttccgcgacc tgccagcgca gcccaagtaa catacagaac agccccagca ggccgccgca
    35881 aatcaccatc tgcaatgcgc caccaccgaa caccgccgca cgcagcttcc acagcctttg
    35941 tggatcgagt tcgaggccga taataaacag catcagcacc acgccaatct cggcaaagtg
    36001 cagaatagat tcggcatcgg tcaccagtcg cagcccccac gggccaataa tgcagcctgc
    36061 aatcaggtag ccaagtaccg atcccagacc aagacgtacc gcaatgggta caatcagcgc
    36121 cgccgaaccg agataaatca gcgcctgaat cagcgtatgg ctatccatga tgtgcctcct
    36181 gccattccag cagacgttgc ttatagtgac gcgcctgccc ttcgagggtt tcgtcgtcac
    36241 aaataaaggt gcagtgcatg gcaaacggcg gcagccagtt cagcccgcag tagattgccg
    36301 tcgcctgtag cggctgcgac agcacatcaa agcccggatg cgcaccaatt tcaaaatggc
    36361 tttccccgcc gccggtcgtc accgcccaca gcaaatgttt gccatgcagc gccgtaccgc
    36421 cgtgaccgta agcccagccg tgcgagaaaa ctttatcgat ccaaagtttg aggagcggtg
    36481 gaatgctgta ccactgcatc gggtgctgca agacgatcag atcggcgcga gacagcgcct
    36541 cctgctcggc ggcaatatcg atattgaagt caggatagag ttgataaaga gagcgaattt
    36601 cgacgccttc cagcgtcctt gcctgttcaa gcatccgttt attcgcatgg gaatgatgcg
    36661 gatacggatg cgcataaatt atgaggatca tagttattag cccgcgtgat tacgcctttg
    36721 ttttcccaca aagtgtagtc atatattcac gcagcttata gtcaataata ttgattcaga
    36781 aaagagagaa atctgaacaa attatcccat tctctgcccg cggatcgtca tattgctggt
    36841 ctgcgccagt gacattcctc gagtctccgg ggcaaacgct acggaaatca acaagccaaa
    36901 cagcgagata cccgccccca ttagcatcgt gttactgata ccgtaattat tgataaagat
    36961 cggtagtgcc caggtcgaaa caatggtgcc aatacggctt aaggacataa tcacgcccac
    37021 ggcagaggcg cggatatctg tcgggaagag ttcattagga tagagccact gcaaattacc
    37081 cggcccgcca gagaaaaagg catacaccgc aaaggccatc actaccagcc agatccccat
    37141 atccgggatt agccccaaaa ccgccagcgc cagcgtcatc atggcaaagc tgccaatcaa
    37201 caatggacgc cgtccagcag tgtttagcca cagcatcggc ggaatacagc cgagcataaa
    37261 gaacaggcta atcaccacat tccccagtgc cgcgtttttg ccaaccccca atcccaacaa
    37321 accaacgatt tgcggaccaa aggtgtaaat ggcgaacatt gggatcacct ggcaggtcca
    37381 gatggcggca acaaacagaa caaaaggaaa atggcggcga ttaaacagat cgcgaaaacg
    37441 ggtttgctgc ggctgctctt catcgaaagc caccggttcg ccaaacagtt ttatcatcat
    37501 ctcttcgcac tcttttactc gccctttgcg taataaccag cggggagatt caggcagttc
    37561 gaatcgacca atcaaaatca acaaacaggg gatcgctgcg ctacccagca tccagcgcca
    37621 gccgccttcc acatcataaa gccagtagcc gaccagatcg gcgcaggtcg cgccgacata
    37681 ccacatggcg gcgataaagc tgatggaaaa tgcccgctga cgggtactgg agaactcggt
    37741 gatcattgag gtggcgatgg gataatctgc accgatgaca atgccgataa gtacccgcat
    37801 caccaacagt tcgacggggg atgaaacaaa caccgtcgcc accgatatca cgccgatggc
    37861 gatgatatca atgaggaaca ttttgcgccg tccgacttta tcggagatat agccaaacag
    37921 cgatgtgcca acgaacagcc cggcaagcgt tcccgcgccc agcaagccaa tccagtcagc
    37981 gtccagtttc agcgtcgggg tcagttgctc cagcgccacg ccaatcatta ccagtacata
    38041 accatccaga aacggaccac cgcttcccca cagcaaaatg cggcggtgaa tagaggagaa
    38101 tttgagatca tcaaagtttc tggacggttg catgggttat tcctgttttt tgcaatgaga
    38161 gtcagtgcgg cgttacgtat caaaccaaca tcagccgtaa cgtaactcca caccaaaggt
    38221 gccgcgcggg tattcccact gttccagcgc cgaccccagc cccagaatgc gacaggtgcc
    38281 gcactccaga catccggcgt aatcgaagcg cacactgccg tcatcctgct tcttgtacag
    38341 acctgcgggg cacgctttca ccagcagctc cagggcctgt ttatcggcat cggccttcac
    38401 aacaatgtgc ggatgctctt catcgacatt gaatttattg acgcccagtt tgacgtccac
    38461 attgacggga gaagtcataa aacggtcact cctttcatgc catccttgat cagattgatg
    38521 aagcccactt tcttgccgtg gcggaggatt ttcttgcgca tcagttccgg cgcactgccg
    38581 tcaatggtga acaggtcacg cgccacgccc accgccagtt ctgggtagcc gctaaacatg
    38641 cgtgggttat caaggaacgc cggtagtttc tggtacatgc gcatatcgcg cagcgggcca
    38701 ctctccagat gctgacgata ttccgcgagt ttttgcttac tgaaatcgtc gcttttcatc
    38761 gctgaaagca cggtttttgc tgcggcttcc ccggcggcaa tcgccagatc cataccgcga
    38821 atggtaaaac cgaggttcat acacattccg gcggcatcac cggcaatcag tacgccgtca
    38881 ccaaccaact ccggcagcat gttgatgcct gcttccggca ctacgtgagc ggaatattcc
    38941 accagcttgc cgcccgcgat cagcggtgca acggccggat gttgtttgaa atcttccagc
    39001 atttgcggca ccgatttttt cgcgtcatgc agatgatgca aaccacaaac cagccccagc
    39061 gacagggtat tttcattggt ataaaggaag ccgccgccca tcaggccatc ggtgggtgat
    39121 cccgcaaaca ggcaagccgc gccctgatta ccctgcaact gaaaacggtc ttcgattacc
    39181 gacttcggta actcgatcag ttccttcacg ccaaccgcca catccgtcgg tttaacgcgt
    39241 tttgccatcc ccagcttttc ggcaagaatg gagttcaccc cgtcagcaag gatcaccgtt
    39301 ttcgcttcaa tcacatcgcc atcggcttct acgccgacaa ctttgccatc gcgctgtacg
    39361 aggttatcga cgcggatccc ggtaattaac tgcgcgcccg cttcttcggc ctgctccatc
    39421 aaccaggcat caaatttact gcgcaaaacg gagtaagaac gctgggatgg cgaggtttcg
    39481 tcaccgttgc agtagtccat agtcatcgct gacttttccg tcataaacgc gagtttttca
    39541 tgggtgatca ggcgttctac gggggcggat tcggcgaaac ctggaataat gtgttccaga
    39601 ctgtgggcat agagacgccc accagtaacg ttcttggcac ctgcggaatt gccacgctcg
    39661 ataactaaca cttgcgcccc ttcgcgggcg agcaccagtg cggcaaccga accggcaagc
    39721 cctgcaccga cgatgatggc gtcaaagata tcttcggaca taagagctcc ctgtcagcgg
    39781 cagagccgta atgcagccct gccagagtgg atcaacgcgc taaagctgcg gtcagtgccg
    39841 gaaggatctt cacggcatcg ccaacaatgc cgtaatccgc gtactggaag atcggcgcat
    39901 ttttatcttt attgatggcg aaaatggttt gcgatgcatt agcaccaacc atgtgctgga
    39961 tctgcccgga gatccccacc gccagataca attcaggttt cagcatcagg ttggagatac
    40021 cgacatagcg ttcgtgctcc atccattttt cgttttccgc caccggacgg gaacaggcca
    40081 actccgcacc tatcgccttg caaagctgtt ctgccagcgc aatgttctct ttgctgccaa
    40141 tgccgcgacc gacgctgacc accagacggg ctttgtcgag atcgacgctg ttgctctggc
    40201 gcgcctgggt tgccgtgcgg gtaatcgcca cagccggagc ctgccactcc acggtgtgca
    40261 tttcgccagt gcgtgacgcg tctggctgag ctgcatcgaa cgtgccactg ctgatggtca
    40321 gtaccgcata cggcgtggca atgcgttctt cgccaatcgc cagaccgccg taaaccatgt
    40381 gtttcactgt cgctttaccg tcctgtacgc tgacggtgct ggcatcgtta gacaccaccg
    40441 ctttaaggcg ataacccagt tttgccgcca gtaatttgcc gcgacgggta ttcggcaaca
    40501 gcaccagacc gtctgcgccg tgctggcgaa tagtgtccgc catgacaccg gcgtaatctt
    40561 cgatcatccg atcgtccggt ttgccgttta atttccagac atgattagcg cctagctgga
    40621 ttgcctgtgc gccgtcggca tcattgagga caaaggcatt gatttgatta gctaaagcct
    40681 gcgcaccgtt catcagttcc ggcagacgag aaggggtatc gctgaatacc cagacttgag
    40741 aaaacgtgtt catagcatcc cctgtaatta aatgactttg cgaagatttt cagcaaatgc
    40801 ggcgatctgt tcttcgccgt cgccttcaat cacgatgcgc tgacgctcgc gctgtttcgg
    40861 tgcggcaacc tgttgttctg accaggctgc ctctgcgtta aaaccgatat ccgccgccga
    40921 ccatacctgg actggctttt tcgccgcgcc gagaatggct ttcatcgaag gaatttgtgg
    40981 ggagttgata tcagtggaaa cagcaacaac cgcaggcagc ggaatgctta aggtttctgt
    41041 ttcatcttcc agttcgcgct caacggtgag ggtatctgcc gtcagggaga taattttgct
    41101 gacgccgtta actgccggaa tattgaggat ttcgcctacc agcaaaccaa cctgctgggc
    41161 ataaaggtcg gaagaaccat cgccacagag gatcaaatca aagcctgctt tctgggcggc
    41221 tgcagccagt gcgctcgccg tttgttgcgg cagtgcctgc tcgaactggt catcaatcac
    41281 cacaatcagt tcatccggac cgcgcgatag cacatcttta cgccctttgg cgttggtcag
    41341 ggctttaccg cccacactta gggccgtcac ctgcgcctct gctgcctgtt gctttagctg
    41401 gcaagccgct tcaatagcgt tgagatcgta ttggcttatt ttggcatcgg ctttactgaa
    41461 gtctaacgaa ccatcagcat tattgaccgc aatatcctgt tcatcaggca cgcacttata
    41521 gcaagtaata atcttcattg catctccaga aatcatgaag gaaacgtcgt tcatatcggt
    41581 ggtgttaatt acagatatca cgccctttaa tttggaaata aaactttaat caccaatatt
    41641 gaaaatgtca cacgcagata aatcca
//
LOCUS       DBANUU010000027        41510 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000027 DBANUU010000000
VERSION     DBANUU010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.027"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(144..356)
                     /gene="cspH"
                     /locus_tag="ACIE68_001388"
     CDS             complement(144..356)
                     /gene="cspH"
                     /locus_tag="ACIE68_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFX5907732.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            642..854
                     /gene="cspG"
                     /locus_tag="ACIE68_001389"
     CDS             642..854
                     /gene="cspG"
                     /locus_tag="ACIE68_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFX5907733.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            865..1053
                     /gene="ymcF"
                     /locus_tag="ACIE68_001390"
     CDS             865..1053
                     /gene="ymcF"
                     /locus_tag="ACIE68_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFX5907734.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            1028..1258
                     /gene="ymcE"
                     /locus_tag="ACIE68_001391"
     CDS             1028..1258
                     /gene="ymcE"
                     /locus_tag="ACIE68_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFX5907735.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            1248..1421
                     /gene="gnsA"
                     /locus_tag="ACIE68_001392"
     CDS             1248..1421
                     /gene="gnsA"
                     /locus_tag="ACIE68_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFX5907736.1"
                     /translation="MNIEELKKQAETEIVDFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1470..2543)
                     /gene="yccM"
                     /locus_tag="ACIE68_001393"
     CDS             complement(1470..2543)
                     /gene="yccM"
                     /locus_tag="ACIE68_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFX5907737.1"
                     /translation="MAENKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHVAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(2615..5359)
                     /gene="torS"
                     /locus_tag="ACIE68_001394"
     CDS             complement(2615..5359)
                     /gene="torS"
                     /locus_tag="ACIE68_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFX5907738.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITIEMLKTSGAQIVAVGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5442..6470
                     /gene="torT"
                     /locus_tag="ACIE68_001395"
     CDS             5442..6470
                     /gene="torT"
                     /locus_tag="ACIE68_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFX5907739.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6443..7135)
                     /gene="torR"
                     /locus_tag="ACIE68_001396"
     CDS             complement(6443..7135)
                     /gene="torR"
                     /locus_tag="ACIE68_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFX5907740.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            7265..8437
                     /gene="torC"
                     /locus_tag="ACIE68_001397"
     CDS             7265..8437
                     /gene="torC"
                     /locus_tag="ACIE68_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFX5907741.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDMPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            8437..10983
                     /gene="torA"
                     /locus_tag="ACIE68_001398"
     CDS             8437..10983
                     /gene="torA"
                     /locus_tag="ACIE68_001398"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFX5907742.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDISQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            10980..11579
                     /gene="torD"
                     /locus_tag="ACIE68_001399"
     CDS             10980..11579
                     /gene="torD"
                     /locus_tag="ACIE68_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFX5907743.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVVRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(11672..11977)
                     /gene="cbpM"
                     /locus_tag="ACIE68_001400"
     CDS             complement(11672..11977)
                     /gene="cbpM"
                     /locus_tag="ACIE68_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFX5907744.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11977..12897)
                     /gene="cbpA"
                     /locus_tag="ACIE68_001401"
     CDS             complement(11977..12897)
                     /gene="cbpA"
                     /locus_tag="ACIE68_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFX5907745.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            13157..14416
                     /locus_tag="ACIE68_001402"
     CDS             13157..14416
                     /locus_tag="ACIE68_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HFX5907746.1"
                     /translation="MSSNIHEISCTANNYLKQAWNNIKNEHEKNQKYSITLFENTLVC
                     FMRLYKEIRRQKAEDYIPCLECDSLEKEFEEMQNDNDLSLFLRTLRTNDTETYSGVSE
                     GITYTIQYVRDIDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEWINTPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFNDLREDVKTLFGVNLQS
                     DNKIQGQGGFLSWSFLSFKKEFIDSCLNDKKCILHLPEFIFNDNKARLVLDTDPEQKV
                     NEVKNFLTALSDSICSLFIVNSNVASISLGNESFSTDDDLEYGYLINTGDHYDVYLPP
                     ELFAQAYELNNKERNAQIDFLTRYAIY"
     gene            14708..15949
                     /gene="agp"
                     /locus_tag="ACIE68_001403"
     CDS             14708..15949
                     /gene="agp"
                     /locus_tag="ACIE68_001403"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFX5907747.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(15987..16214)
                     /gene="yccJ"
                     /locus_tag="ACIE68_001404"
     CDS             complement(15987..16214)
                     /gene="yccJ"
                     /locus_tag="ACIE68_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFX5907748.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(16235..16831)
                     /gene="wrbA"
                     /locus_tag="ACIE68_001405"
     CDS             complement(16235..16831)
                     /gene="wrbA"
                     /locus_tag="ACIE68_001405"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFX5907749.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            17204..17377
                     /gene="ymdF"
                     /locus_tag="ACIE68_001406"
     CDS             17204..17377
                     /gene="ymdF"
                     /locus_tag="ACIE68_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFX5907750.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(17460..18788)
                     /gene="rutG"
                     /locus_tag="ACIE68_001407"
     CDS             complement(17460..18788)
                     /gene="rutG"
                     /locus_tag="ACIE68_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFX5907751.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNLLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(18809..19303)
                     /gene="rutF"
                     /locus_tag="ACIE68_001408"
     CDS             complement(18809..19303)
                     /gene="rutF"
                     /locus_tag="ACIE68_001408"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFX5907752.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(19314..19904)
                     /gene="rutE"
                     /locus_tag="ACIE68_001409"
     CDS             complement(19314..19904)
                     /gene="rutE"
                     /locus_tag="ACIE68_001409"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFX5907753.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(19914..20714)
                     /gene="rutD"
                     /locus_tag="ACIE68_001410"
     CDS             complement(19914..20714)
                     /gene="rutD"
                     /locus_tag="ACIE68_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFX5907754.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARVPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(20722..21108)
                     /gene="rutC"
                     /locus_tag="ACIE68_001411"
     CDS             complement(20722..21108)
                     /gene="rutC"
                     /locus_tag="ACIE68_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFX5907755.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(21120..21812)
                     /gene="rutB"
                     /locus_tag="ACIE68_001412"
     CDS             complement(21120..21812)
                     /gene="rutB"
                     /locus_tag="ACIE68_001412"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFX5907756.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(21812..22903)
                     /gene="rutA"
                     /locus_tag="ACIE68_001413"
     CDS             complement(21812..22903)
                     /gene="rutA"
                     /locus_tag="ACIE68_001413"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFX5907757.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            23191..23829
                     /gene="rutR"
                     /locus_tag="ACIE68_001414"
     CDS             23191..23829
                     /gene="rutR"
                     /locus_tag="ACIE68_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFX5907758.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(23869..27831)
                     /gene="putA"
                     /locus_tag="ACIE68_001415"
     CDS             complement(23869..27831)
                     /gene="putA"
                     /locus_tag="ACIE68_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFX5907759.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVMCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            28253..29761
                     /gene="putP"
                     /locus_tag="ACIE68_001416"
     CDS             28253..29761
                     /gene="putP"
                     /locus_tag="ACIE68_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFX5907760.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            30304..31134
                     /gene="efeU"
                     /locus_tag="ACIE68_001417"
     CDS             30304..31134
                     /gene="efeU"
                     /locus_tag="ACIE68_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFX5907761.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            31192..32319
                     /gene="efeO"
                     /locus_tag="ACIE68_001418"
     CDS             31192..32319
                     /gene="efeO"
                     /locus_tag="ACIE68_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFX5907762.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            32325..33596
                     /gene="efeB"
                     /locus_tag="ACIE68_001419"
     CDS             32325..33596
                     /gene="efeB"
                     /locus_tag="ACIE68_001419"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFX5907763.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            34215..35003
                     /gene="phoH"
                     /locus_tag="ACIE68_001420"
     CDS             34215..35003
                     /gene="phoH"
                     /locus_tag="ACIE68_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFX5907764.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIIRFGKEDCVRSALCQRTLHAYS"
     gene            complement(35053..35466)
                     /gene="pgaD"
                     /locus_tag="ACIE68_001421"
     CDS             complement(35053..35466)
                     /gene="pgaD"
                     /locus_tag="ACIE68_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFX5907765.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(35468..36793)
                     /gene="pgaC"
                     /locus_tag="ACIE68_001422"
     CDS             complement(35468..36793)
                     /gene="pgaC"
                     /locus_tag="ACIE68_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFX5907766.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDRTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            complement(36786..38804)
                     /gene="pgaB"
                     /locus_tag="ACIE68_001423"
     CDS             complement(36786..38804)
                     /gene="pgaB"
                     /locus_tag="ACIE68_001423"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFX5907767.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSKTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADEQVKFGDEMVD
                     REYFATWQQVREVARSPLVEVASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAKVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANSSQLDSIPRVLIANNPSLKEFAQQIITVQEKSSQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDKVRAQVGMLY
                     EDLAGHAAFDGLLFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHDQPEIDVIRPEFSTAWYPKND"
     gene            complement(38813..41236)
                     /gene="pgaA"
                     /locus_tag="ACIE68_001424"
     CDS             complement(38813..41236)
                     /gene="pgaA"
                     /locus_tag="ACIE68_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFX5907768.1"
                     /translation="MYSSSRKRCPKTKWALKILTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALLWFAQKSALSNNQIADWLQIALWAGQDKQVITVYNRYRYQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERFFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSID
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            complement(41329..>41510)
                     /locus_tag="ACIE68_001425"
     CDS             complement(41329..>41510)
                     /locus_tag="ACIE68_001425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5907769.1"
                     /translation="TDLIILILIYFEKGIGIYAVFLKILIIGMDFRARKGFYMDTLLI
                     ISSSHHQQFTSLFRV"
BASE COUNT        10210 a        10393 c        10709 g        10198 t
ORIGIN      
        1 tggcatttgt aactattagg tgcagagata ttcgcttaaa tggattagtt tctttgtgga
       61 aaagtaactg atgttataat gtatgatgtt gactattgca ctattccaat aaaaccagtc
      121 agctttaaac aagcagcgtc atattaagag agataaacat ttgccgctgt tggtcctcgc
      181 aggccattta cgcggcaaaa ttccacgcgt aatcctggta taagcacttc tgcgtcgcgg
      241 ggagtgaatg cggaaatatg gacctggact tctttacgac cgtcggaggg gataatgaat
      301 cctttgccgc ttttgcgatc aaaggttttg acaattcctg tcattttacg ggacaaacaa
      361 attccttact gaaaatactg tgccgcacta tacggggtta ataaaataaa gccagcgata
      421 tttaagaccg ccggacggct aaaataaaat ttgcttaatc tcaattatca tgcgttaata
      481 gctgcgtcgg tttgaaagac agacagcata caaagtagtt tactaaagca gttctcatta
      541 tcaggcatta tccctttctt ttgagtctct ctcctgaaca ctaagtagtt tctgtattaa
      601 agccctgttt gccgaaaggc ccaaaatgaa ggaagtaaaa tatgtctaat aaaatgactg
      661 gtttagtaaa atggtttaac gcagataaag gttttggctt tatcactcct gatgatggca
      721 gcaaagacgt tttcgtccat ttcaccgcca ttcagagcaa tgaattccgc acgctgaacg
      781 aaaatcagaa agttgaattt tctattgagc aggggcaacg tggccccgcg gcagcgaacg
      841 ttgttacgct ctaaggttgc cgttattact caacatctcc atttccgctg tccatgttgt
      901 catggttcac agtaccgtac atcggcattc gatgtgacgg agcgaaaccc tttgggcgct
      961 aagtgtattt tttgtaaatc gacgatgatc acctttgata acgtcgcgct gcaaatacgc
     1021 acggaccatg cgccgctgga tttcacaaaa taatatcagg ctccctcgtg gagccttttt
     1081 tatatctgcc ttatttttct tcaacgctgt atgtatagta agcgataacc tgttgattat
     1141 tgaatctttc ggggagatgg cttataacat ttcttacctg accagggtac cgggaaccaa
     1201 caccttactg gcgtgttgct gtctttcaag accagacgag gttaacagtg aatattgaag
     1261 agttaaaaaa acaagccgaa acggaaatcg ttgactttat cgcgcaaaaa atcgccgagc
     1321 tgaacaagaa tacagggaaa gaagtctctg aaattcgctt caccgcacga gaaaaaatga
     1381 ccgggcttga aagttatgat gtcaaaatca aaataatgtg attttgtgaa catcaccccg
     1441 tgcgaggtga tgttccgctt gttgctaatt tagtgaccaa tcattggcgc ttgtggaatt
     1501 aagcgtcggt acaattcctc cggcaccggg ctttgccata ctcccgcata cattgcgtaa
     1561 ccaatcaccg caaacataat ccccagaacc agtagcgtca ttaaccagcc agacaacgca
     1621 aaagcttttt tatttgccgc aggtttttgc agtgaaaagg tcaatgttga ggctaccgga
     1681 catgactcta cgcaagtcat acagccggta cattccactg ttcgtacctg aattaattta
     1741 tctaccggga tccgtgatgg gcaatttttt gcgcatttgc cacagtcgat acaactttcg
     1801 gcattgcgac gaatcttaaa cggcgacaat agcgaaacca cgcccatcag cgcgccatat
     1861 gggcaaagat aacgacacca ggcatggcga ataaacaggc tggcaatcag caaaacggtc
     1921 acgctgatta atgtcgcggt ccccatatga cgaaagaaat cgagcatttt tacgtccatc
     1981 accacgctgt agggcgacaa cataaaatag tgaatcgcct gagcgggcat caataacgcg
     2041 atatagagaa aaaaactcaa cagcaaatac ttcacgccgc gcagaggaat atccagccag
     2101 cggggaagga cacattgccg accaaacagt ttgttaccga gatcgccgat taattcagaa
     2161 agcgtaccaa ccgggcataa ccatgagcaa aaggcctttt tgagtaatag actgatgacg
     2221 ataaaagcga ccaataacag catcgcggcg acgtggacgg acggtaactg acctgttgta
     2281 aggctatatt tcagattcat cagaccggca atcggtagcc agccttcgat acctcccggt
     2341 ctggcgacaa atgtcgtgct acttgccgtt tcgtaatagc gcacccaata ccagaacgtg
     2401 atggcaatat aaatattcat tgccaacagt aataattgcg tcgctttacg ccaggtcgtg
     2461 gcattacgcc agtcattcca cggtaatttg ccgcccgtcg tgcctggccg ccgctgccag
     2521 cgggttcttt tattctctgc catgattttg ccagtccgtt aagttgtata ccaaatgcca
     2581 ctattctagt tgttcttaac tggctgatat tgattcaaat cgcgttcagg tctttcttat
     2641 gcaaccatgc ttccagagcg gcaacactgc gtgtaatttc ttcgtgtgga aggggggcag
     2701 ataatggctg ctgctccagt tgtgcgcata gctggctggc gatacgcatt cccagactcg
     2761 agcaactgct ttttagctga tgcgcggcac gctttatttt ctcgctatcc tgactggcgc
     2821 gggcaatgtc gatttcatcg agaagcggca gggcatgttg tgtaaataat accagccatt
     2881 cgtggatctt ctccgtcccc attaactgag catcttcatt gagttgcgat acatccagcg
     2941 attgatcatt attgacttgc agttggagat agtgcgccag taactgaccg agcacttcac
     3001 gcggcaccgg tttagggata atcccgcgga ataatgaact ggtacgctgg cgcagcgttt
     3061 cgtcaatgac atgggcgcta aagccaatca aaaccagcga cggatattgc tgtgccagtt
     3121 gtcgggcaag cgtaatgccg tcgatatccg gcagatcaaa atccaccagt gcggcagcaa
     3181 acggttcgct attttgcagt gtctctaaag cctgcgcggc attgccaaca gcaacaatct
     3241 gcgcaccact ggttttcagc atctcaatgg taattcgctg ggttagcggg ttatcttcaa
     3301 ttaacagcaa acgtaaaccg tcaagacgca ccgcctgatt gactgttttt ggcacgggtg
     3361 ccgtggcaac acgtaacggc aagcgtaaac aaaaacagct tccaacctcc ggcgtgctgg
     3421 tggcgctcag ttcgccgccc atcgcctggg ccagacggct actgatagtc agtcccagcc
     3481 cggtgccgcc gcgtttgccg cttacctgca caaatggctg gaagatttct gccagtttcg
     3541 cgggatcaat accgcagccg ctgtcttcca cttcgaccag ccattgctcg ccatcagtgc
     3601 gactacgcag gataatgtac ccttcgtcag taaaacgcag ggcgttgctc aacaggttgg
     3661 ttataacctg acgaatacgt cgtggatcgc ccattaacgc gcacggcata tcatcggcaa
     3721 ttgccgttgc caggcgaatc gggcgacctt tcacccgtcc gctcattaat tgcagggtac
     3781 tttccagcag cgggcgcggt tcaaagggct catcgctgac cgaaacattc ttgccacctg
     3841 cttcgatagc ggaataatcg agaatatcgt tgaggatggt cagcaacgat tcaccagagt
     3901 cagtaattgc ccgcaaatca tcacgctggg cgttaagtgc ggggttatct gccagtagct
     3961 gtgcggtgcc gagaataccg tacagcggtg tgcggatctc atggctcatc gccgccagaa
     4021 acgccgattt tgcctggctg gctttttctg cttccgcccg tgcctgtcgg tgttctatca
     4081 ccagttcctg caattcagct gtacgcgctt tgacctgcgc cgccagctgt tcgcggtggc
     4141 gattcagtgc atgaacattg ctgcgaaacg catccatcag ccgcccgatg gtatccagct
     4201 cccgtacgcc agcggtttcc gggaaagggg agtcaatatc accgtccagc agccgttgca
     4261 gcgcctgcgt ttgttcggca agtggacgcg tgactgagcg ataaaccacg cgccagagga
     4321 tcagaatcag tgcgcaaagt gaaaccatcc ccagcaataa caggctgtat tgcccgcgtg
     4381 cactggcttt ttccagatgt gccagtccat gctgattgcg cagctcaatg gtgtcgacca
     4441 gctgactgac ttcgctacta aactgcgcga actgggcgat attattttgg gcgagagttt
     4501 gcaggtgatt gctgatttca ctgtcctgct ggaacagcgc caacagatcg ctatattggc
     4561 taacggtagt taacgttgtt gcgacctgcg cacgaacacc cggatcttca atgcgtattt
     4621 gccgacgttg cagaattttc accgcattat tgagctgctt ttccagcgtt ggcgcatttt
     4681 tctggatctg ctccagcccc agattcatca ccatttgctg cacccgcagg gcgctaaggc
     4741 gcagttcatt catctggtta acatactcaa gatcgatatc aatcagccga tcgagtgcac
     4801 tttctgcggc ctgacgctga tcttgttcga tcaaatcgta aatcccggcc tgggtcgctc
     4861 cggcggaggt tgccgcatta ttcgcctgac cttgcgccag gcgtgcgatc tcatcggcgg
     4921 cagcgactat ctgctgactg agttgctgtt gttgctggcg cagttgcaaa cgctgcccca
     4981 ccagttcccc ttgctgacgt aacgaacggg agatctcctg ctcctgttgt tcaatagcgg
     5041 tggtatcaaa accttgttcc cgtaacgctt gcagcaacgc attaatcttc aggctttgtg
     5101 cggtgagcat tcgcccctgc gcctgccaca tcttttcgtt atcggcactg gtcaggttct
     5161 gcgcggcgaa aagttcccag gcgctggctt cgctcaactg gcgcgccata ttcatggtag
     5221 gaatcaatgc ctgagtattg tctttttcca cctggctgat aaagcgcagg ttgtaccatc
     5281 ccaccagggt actggtcagg gttaacagcg ccatcagggc aaagcccatc cagagtcttc
     5341 gggtcagggt taaattcacg gtcggtgcac tttaggtgaa aaaggttgag tcgcaaagcg
     5401 gaatgcatct agcataaagc cttattattg atgaggctat catgcgcgta ctgctatttt
     5461 tacttctttc ccttttcatg ttgccggcat tttcggctga taacctgttg cgctggcatg
     5521 atgcgcagca tttcacggtg caagcctcta cgccgcttaa agccaaacgc gcatggaaac
     5581 tgtgcgcgct ttatcccagc ctgaaagatt catattggtt atcgttgaac tatggtatgc
     5641 aggaggctgc tcgccgctac ggtgtggatt taaaagtgct ggaggcaggc ggctacagcc
     5701 agttggctac ccagcaagca caaatcgacc agtgtaaaca gtggggcgca gaggccattt
     5761 tgctcggtag tagcacgacc tcatttcccg acctgcaaaa gcaggtagca agtctgccgg
     5821 tgatcgaact ggtaaatgct attgatgctc cccaggtgaa aagccgcgtt ggtgtgccct
     5881 ggtttcagat gggctatcaa ccggggcgat atctggtgca atgggcgcac ggtaaaccac
     5941 tgaatgtgct gttgatgccc ggacccgata acgccggggg cagtaaggag atggtcgagg
     6001 gttttcgcgc agccattgcc ggaagcccgg tgcgtattgt tgatattgcg cttggtgata
     6061 acgatattga aatccagcgt aacctgttgc aggagatgct ggaacgccat ccagaaatcg
     6121 acgtcgttgc cggaacggcc attgcggcag aggcggcaat gggggaaggg cgtaacctga
     6181 aaacgccgct taccgtggtg tcgttttatc tttcacatca ggtgtatcgc gggctgaagc
     6241 ggggaagagt gattatggct gccagcgatc aaatggtctg gcagggggaa ctggcagttg
     6301 agcaggccat caggcaatta caggggcaat cggtgtctga taatgtcagc ccaccgattt
     6361 tagttctgac gccgaaaaat gccgaccgag aacatatccg ccgctcgctg tcaccggggg
     6421 gatttcgtcc ggtctatttt tatcagcaca catcagcggc taagaaataa ccttcaccat
     6481 gttgcgtcac cagtaaatcc gcgctgagtt tatgacgtaa acgacgaatt aacacatcga
     6541 cggtgcgcag gtcagggttt tccacccgac gcgcagaaag catacgtagc agacgttcac
     6601 ggctgagaat ttcgcccgga ttcgtcacaa atgccaccaa catttcatac tctgcgcggg
     6661 tcagtttaat cggctcgcca tcccgctcca gcgtatggcg cgacacattc aggcaataac
     6721 cggcaaagcg atagcagttg tcctgagtgt gcggttgagc ttgtcgcgcg aggtcgattc
     6781 gccagagcag atttttcacc cgtactacca gttcgcgcag ttccagcggt ttggtgacgt
     6841 aatcgtctgc gcccatttcc agcccaacaa tacggtcaat ccgatcgctg cgtccggtaa
     6901 ccagaataat ccccaccgtt gagcgttctc gcagggcgcg ggttaacatc aggccatttt
     6961 catcgggtaa gttgatatcc agcagaatta aatctaccgg ctgattctgc ataatttccc
     7021 gcagcccggc accgctcgct gtaacggaaa cggtataccc ctcctgagtg aagtaagatt
     7081 gtaatcgcgc ctgggtaacc ggctcatctt caacaataac aatgtgatgt ggcatcagag
     7141 ggttttactc attctgttca tatctgttca tattctgccg taagccgttc atcctgacca
     7201 gtgccgctgt tcatatttgc tcattaagat cgcttcacta aaccataatt ttacaggggc
     7261 tattatgcgg aaactctgga acgcgctacg ccgacccagt gctcgttggt cggtactggc
     7321 gctggtcgca attgggattg tgattggcat tgcgctgatt gtattgccac acgttgggat
     7381 caaagtcacc agcacaaccg aattttgtgt cagttgccac agtatgcaac cggtgtatga
     7441 agaatataaa cagtcggtgc atttccagaa cgcctccggc gtgcgagctg aatgccatga
     7501 ctgtcatatc ccgccggata tgccaggcat ggtgaagcgt aaactggaag cgagcaatga
     7561 tatctaccag acctttattg ctcactccat tgatacacct gaaaaattcg aagccaaacg
     7621 cgcggaactt gccgagcgtg aatgggcgcg aatgaaagaa aacaactcgg caacctgccg
     7681 ctcctgccat aactacgatg cgatggatca tgcgaagcag catcctgaag cggcacgtca
     7741 gatgaaggtg gcagcgaaag ataatcaatc ctgcatcgac tgtcataaag gtattgccca
     7801 ccagttaccg gatatgagta gcggcttccg taagcagttc gatgagctgc gcgccagtgc
     7861 taatgacagt ggtgacacgc tgtactctat tgatattaag ccaatttatg cggcgaaagg
     7921 cgataaagaa gcctctggtt ctctgctgcc tgcttcggaa gtgaaagtcc ttaaacgtga
     7981 cggcgactgg ctgcaaattg aaattaccgg ctggacggaa agcgccggac gtcagcgtgt
     8041 actcacccaa ttcccaggta aacgcatctt tgttgcctcg attcgtggtg atgtgcagca
     8101 gcaggtaaaa acgctggaga aaaccaccgt tgccgacacc aataccgagt ggagcaagtt
     8161 gcaggccact gcgtggatga agaaaggcga catggtgaac gatatcaaac cgatctgggc
     8221 ttatgcggat tcgttgtaca acggcacctg taaccagtgc cacggcgcac cggaaatcgc
     8281 ccactttgac gctaacggtt ggatcggcac gctcaacggc atgattggct ttaccagtct
     8341 cgataaacgt gaagaacgca ccttgttgaa atatctgcaa atgaatgcgt ctgacaccgc
     8401 aggtaaggct cacggcgata agaaggaaga aaaataatga acaataacga tctctttcag
     8461 gcatcacgtc ggcgttttct ggcacaactc ggcggcttaa ccgtcgccgg gatgctgggg
     8521 ccgtcattgt taacgccgcg ccgtgcgact gcggcgcaag cggcgactga cgctgtcatc
     8581 tcgaaagagg gcattcttac cgggtcgcac tggggggcta tccgcgcgac ggtgaaggat
     8641 ggtcgctttg tggcggcaaa accgttcgaa ctggataaat atccgtcgaa aatgattgcc
     8701 ggattgccgg atcacgtaca caacgcggcg cgtattcgtt atccgatggt acgcgtggac
     8761 tggctgcgta agcgccatct cagcgatatc tcccagcgcg gtgataaccg ttttgtgcgc
     8821 gtgagctggg atgaagccct cgacatgttc tatgaagaac tggaacgcgt gcagaaaact
     8881 cacgggccga gtgccttgct gaccgccagt ggttggcaat cgacggggat gttccataac
     8941 gcttcgggga tgctggcgaa agctattgcc ttgcatggta atagcgttgg tacgggcgga
     9001 gattactcta ccggtgctgc gcaggtgatc ctgccgcgcg tggttggctc aatggaagtg
     9061 tatgaacagc aaacctcctg gccgctggta ttgcagaaca gcaaaaccat tgtgctgtgg
     9121 ggctctgatt tgctgaaaaa ccagcaagcg aactggtggt gcccggatca cgatgtttat
     9181 gaatattacg cgcagctaaa agcgaaagtc gccgccggtg aaattgaggt catcagcatc
     9241 gatccggttg tcacatccac ccatgagtat ctggggcgcg agcatgtgaa gcacattgcg
     9301 gttaacccgc aaactgatgt gccgctgcaa ctggcgctgg cgtatacgct gtacagtgaa
     9361 aacctgtacg acaaaaactt cctcgctaac tactgtgtgg gttttgagca gttcctgccg
     9421 tacctgctgg gtgagaaaga cggtcagccg aaagatgccg catgggctga aaaactgacc
     9481 ggcattgatg ccgaaaccat tcgtgggctg gcgcggcaga tggcggcgaa cagaacgcaa
     9541 attattgctg gctggtgcgt gcagcgtatg cagcacggtg aacagtgggc gtggatgatt
     9601 gtggttctgg cggcgatgct ggggcaaatt ggcctgccag gtggtggctt tggttttggc
     9661 tggcactata acggcgcagg cacgccgggg cgtaaaggcg ttattcttag tggtttctcc
     9721 ggctctacgt cgattccgcc tgttcacgac aacagtgatt acaaaggcta cagcagcacc
     9781 attccgattg cccgttttat cgatgcgatc ctcgaaccgg ggaaagtgat caactggaac
     9841 ggtaaatcgg taaaactgcc gccgctgaaa atgtgtattt ttgccggaac taacccattc
     9901 catcgccatc agcagatcaa ccgcattatt gaaggctggc gcaagctgga aacggttatc
     9961 gccatagata accagtggac ctcaacctgc cgctttgccg atatcgtgct gcctgcgacc
    10021 acgcagtttg agcgtaacga tctcgaccag tacggcaacc actccaaccg tggcattatc
    10081 gccatgaaac aggtggtgcc gccgcagttc gaggcgcgca acgactttga tattttccgc
    10141 gagctgtgcc gtcgctttaa tcgcgaagaa gcctttaccg aagggctgga cgaaatgggc
    10201 tggctgaaac gcatctggca ggaaggtgta caacaaggca aaggacgcgg cgttcatctg
    10261 ccagcgtttg atgacttctg gaataacaaa gagtacgtcg agtttgacca tccgcagatg
    10321 tttgttcgcc accaggcatt ccgcgaagat ccggatctcg aaccgctggg cacgccgagt
    10381 ggcctgattg agatctactc gaaaaccatc gccgatatga actacgacga ttgtcagggg
    10441 catccgatgt ggtttgagaa aatcgaacgc tcccacggtg ggcccggctc gcagaagtat
    10501 ccgttgcatc tgcaatctgt gcatccggat ttccgacttc actcgcagtt atgtgagtcg
    10561 gaaactctgc gtcagcaata tacggtagcg ggtaaagagc cagtgttcat taacccgcag
    10621 gatgccagcg cgcgcggtat tcgtaacggt gatgtggtac gcgtctttaa cgctcgcggt
    10681 caggtgttgg caggggcagt agtttctgac cgctatgcac ccggcgtggc acgaattcac
    10741 gaaggggcat ggtacgatcc agataaaggc ggcgagcctg gtgcactgtg caaatacggt
    10801 aaccccaacg tgctgaccat cgacatcggt acttcgcagc tggcgcaggc gaccagtgcg
    10861 cacactacgc tggtggaaat tgagaagtac aatggagcag tggagcaggt gacggcgttt
    10921 aacggccctg tggagatggt ggcgcagtgc gaatatgttc ccgcgtcgca ggtgaaatca
    10981 tgaccacgct gacagcacaa cagattgcct gtgtttacgc ctggctggcg cagttgttct
    11041 cccgtgaact ggacgatgaa caactgacgc aaatcgccag tgcgcagatg gctgaatggt
    11101 tttcgttgct gaaaagcgaa ccgccgctgg ctgcggcggt gaatgagctg gaaaactgta
    11161 ttgccacgct gacagtacgt gacgatgccc gtctggaact ggccgctgac ttttgcggcc
    11221 tgtttctgat gaccgacaaa caagcggcgc tgccgtatgc atcggcctac aaacaggacg
    11281 agcaagagat taaacgcttg ttagttgagg cagggatgga aaccagcggc aatttcaacg
    11341 aaccggcaga tcatctggcg atctatctcg aattgctcag ccatctgcat ttttcgctgg
    11401 gagaggggac cgttcctgcg cgaagaatcg acagtttgcg gcaaaaaaca ctgacggcgc
    11461 tgtggcaatg gttaccagag tttgttgtgc gttgtcgtca gtatgacagc tttggttttt
    11521 acgcggcact aagccagtta ttgctggtgt tagtggagag cgaccaccaa aacagataac
    11581 gtcgtttgtg cgcctgaaaa gacgcgttta gcgtcgcatc aggcattatg gggcagttgc
    11641 cggatgcgtc atcacatccg gcaaacacac atcacggatg agctacaaac cgggaaagtc
    11701 gctggcgcag caggcggttt tcctgcttca ggtgcgcaat atcatccatt aacgtcagcg
    11761 ccaccgcgat cccaggccag tccagagcca gttcatgacg caggcgtact gcgcgttgca
    11821 ccacgatggc ggcatggtcg tcaaataccc aggttgtttc ctggatctca cgcggttcaa
    11881 ccacccccaa accgacaatt tcattcaact cctcttcaga gatgccggta tgcaggcaaa
    11941 attcggtaat agtaaaagtc accgtaacat tagccattat gctttccccc aatctttacg
    12001 tggatcaaaa gacgactggg cgtctgccag ttgctgccac agcgcggcag tgttttcatc
    12061 cggtttcggc ggcatcacga ttttcagtac cgcatacaga tcgccggtct gttttttgct
    12121 caccagacct ttgcctttaa cgcgcaatcg ttgcccggcc tggctgcctg gcgggatagt
    12181 cagcaaaatg ctttctttca gtgttggaac ggtgacttta gcacccagcg ccgcttccca
    12241 cgggctaacc ggcactacaa tttccagatc atggccgaca atatcgaaca gcggatgtgg
    12301 cgcaatatga atcaccagcc acaaatcgcc atttggaccg ccgttttcgc ccggcgtccc
    12361 ctggcctttc agacggatgc gttgaccatt gccgacgccc gccgggatct tgacattcag
    12421 cgttttcgga atttcctgtt cgatcatgcc aaaggcgtta taaaccggca ggttatagct
    12481 gatggtacgc ttatgctcag taagcgtttc ttcgaggaat accgccactt cgatttcaat
    12541 atcgtggccg cgtgtggcgg ggcgttgacg gctctggcgg gcatgctgac cgaaaattga
    12601 cgagaagata tcgtcaaaat cttcggcgtt aaaactctga ccgtcgctat gatggaactg
    12661 acggttaaat tgcggatcgt tgcgatgttg ccacatctga tcatactcag cgcgacgttg
    12721 ttcatcactt aacacttccc aggcttcagc gacctctttg aagcgggctt cggcatccgg
    12781 ttctttgctg acatcaggat ggtatttgcg ggccagtcga cgataggcgg tcttgattgt
    12841 cttgagatcg tccgtcggtt tcacgcccat gatggcgtaa taatccttta attccatagc
    12901 gttatctcgc gtaaatcaac acaaattgaa ggaacccctg taaggtaact cctataagtg
    12961 tagggtaatc ctcaaaattt catatgccaa cacagaatat gttattgaaa tcatcgcgga
    13021 gaggaggtcg cctcaaggtg gggtgctgaa catattttaa acaggtgaaa aagggtgagc
    13081 gatttttgat agtggaacca ggcactttaa gtttaactgg ggcgtcatta tttattaaat
    13141 tttataggcg ctatatatga gtagtaatat acatgaaatt agttgcactg caaataatta
    13201 tttgaaacaa gcctggaaca atataaaaaa tgagcacgaa aaaaatcaaa aatattcaat
    13261 cacgcttttt gaaaacacac tggtgtgttt tatgcggttg tataaagaaa tcagacgtca
    13321 aaaagctgaa gactacattc catgcctgga atgcgattca ctagaaaaag aatttgagga
    13381 aatgcagaat gataatgatt tatcattatt tttgagaaca ttgcgtacta atgatacaga
    13441 aacttattca ggggtttcag aaggtattac atatactata caatatgttc gagatattga
    13501 tattgttaga gtgtccttgc cgggcagagg ttcagagtct atcacagatt ttaaaggtta
    13561 ttattggtat ggctttatgg aatatattga aaacattaat gcgtgtgatg atgttttttc
    13621 tgagtattgt ttagatgatg aaaatatgag tatccagcca gaatggataa atacgccggg
    13681 aatatctgat ttagatactg gtattgattt gtctggtata tcttttattc agagtgaaat
    13741 taataaaact tatggattaa aatacgctcc agtagatggc gatggctatt gtctgttaag
    13801 ggccatactg gttttaaaag aacatgagta ttcatgggcg ctgggcagcc ataagacgca
    13861 aaagcaagta tacgaagagt ttattaaaat tgttgataaa caaacgatcg aggctcttgt
    13921 tgatacggca ttcaatgatc tcagggaaga tgtaaagaca ttattcggcg ttaatctaca
    13981 atctgacaac aaaattcagg ggcagggtgg ttttttatca tggagttttt tgtcttttaa
    14041 aaaagaattt attgatagtt gcttgaatga caaaaaatgc atactgcatt tacccgagtt
    14101 tatatttaat gataataagg cacgccttgt tttagacacc gacccagagc agaaggttaa
    14161 tgaggtgaaa aatttcctca ctgctctttc agatagcatt tgctcattat ttattgttaa
    14221 tagtaatgtg gcatcaatct ccttagggaa tgaatccttt tcaacagatg acgaccttga
    14281 gtatgggtat ttaataaaca ctggcgatca ttatgacgtt tacctccctc ctgaactttt
    14341 tgctcaggct tacgagttaa acaataagga aaggaatgcg caaattgatt ttttaactcg
    14401 ttatgcaatt tactgacaaa ggcatgtgct aaaaaccatt gttattagcc tcacttctct
    14461 tattattggt actattgcct ctgtattagt aacgccgctg atattctgtt tagccacagg
    14521 tgtaatcatc agcggcgtac gcgaggcagg gagctaatca ggcatagttt gcgtcaaacc
    14581 ttgcctgttt ttaaagatgt atatagaaaa acaggcattc aacaagccat tttgcgaacc
    14641 tgttcccgga aaaatgtcat atttctgtca cactctttag tgattgataa caaaagaggt
    14701 gccaggaatg aacaaaacgc taatcgccgc aactgtggca gggatagttt tactcgcttc
    14761 aaatgctcag gcacaaaccg taccggaagg ctatcagcta cagcaagtgc tcatgatgag
    14821 ccgccataac ttacgtgcgc cgctggcgaa caatggcagt gtgctggagc agtctacgcc
    14881 gaataaatgg cccgaatggg acgtccccgg tgggcaactc accaccaaag gtggcgtgct
    14941 cgaagtgtat atgggccatt acatgcgtga atggctggca cagcagggga tggtgaaatc
    15001 gggggaatgc ccgccgccgg acaccgttta tgcctatgcc aatagtctgc aacgtaccgt
    15061 tgcgaccgca cagttcttta ttaccggcgc attcccgggg tgtgatattc ctgtgcatca
    15121 ccaggaaaaa atgggcacta tggacccaac ctttaacccg gtgatcaccg atgattccgc
    15181 cgcattcagt gaacaggcgg tggcggcaat ggagaaagag ctcagcaaac tccagcttac
    15241 cgacagctac caactactgg aaaaaatcgt taactataaa gattcccctg cctgtaaaga
    15301 gaaacaacag tgttcgctgg tggatggcaa aaataccttt agcgccaagt atcaacaaga
    15361 accaggtgtc tccgggccgc tgaaagtcgg caactcgctg gtagatgcgt ttactttgca
    15421 atattacgaa ggttttccga tggatcaggt ggcctgggga gaaatcaaat ctgaccagca
    15481 gtggaaggtg ttgtcgaagc ttaaaaacgg ctaccaggac agcctgttta cctcaccgga
    15541 agtggcgcgc aatgttgcga aaccgctggt cagttatatc gacaaagctc tggtcaccga
    15601 tcgcaccagc gcaccgaaaa ttacagtgtt ggttgggcac gactccaaca ttgcctctct
    15661 gttaacggcg ctggatttca aaccgtatca gttgcatgac cagaacgaac gcacgccgat
    15721 tggcggcaaa atcgttttcc agcgttggca tgacagcaaa gccaatcgcg atttgatgaa
    15781 aattgaatat gtgtatcaga gtgcggaaca gttacgtaat gccgatgcgt taaccctgca
    15841 ggcacctgct cagcgtgtga cgctggaatt aagcggttgc ccgatagatg ccaacggttt
    15901 ctgcccaatg gataagtttg atagcgtgtt gaatgaggcg gtgaaataac agaaaactcc
    15961 cccgcgagaa gtgggggagt cgctggttaa acgtttttac gttcgatggt ctgttcgccc
    16021 caaaaaagcg aatctttatc ggtcttagca aaggctttga ctaacacttc atcgctacct
    16081 tcttcccaaa tcttttccgc cattttttcg tcgtacccgg cgacttcgaa aatggcctcg
    16141 gctatttccg gcgacgtatt gcgcagagat gcccattcac cgacgtgatg agctttcgct
    16201 tcttgagttg gcatgcgtat cctcctgttg aagattagcc gttaagttta actgccagac
    16261 ctgcgacata ttccccttga taacgagcaa tagacagttc ttcctggctt ggctggcgtg
    16321 agccgtcacc gcctgcgatg gtggttgcgc cgtacggcgt accgccgcga acctgtgaaa
    16381 cgtcaaataa ttcctgcgct gcgtagccaa tggggacaat taccatgccg tgatgcgcaa
    16441 gggtcgtcca ggttgaagta atagtttgtt cctgaccgcc gccagtaccg gtggaactaa
    16501 agacgctcgc cagttttccg tatagtgcgc cggaagccca caggccgccc gtctggtcga
    16561 ggaaggtacg catttgaccg gacatgttgc caaagcgggt aggtgtacca aaaataatgg
    16621 cgtcgtaatc ggccagttct tgcggggttg caaccggtgc agtttgcgtt ttaccgcctg
    16681 ctttttcaaa taattgcggc ggcatggttt ccggtacacg cttaacgaca acttctgcgc
    16741 catcgacttt gcttgcaccc tcagcgactg cgcgtgccat cgtttcaata tgtccgtaca
    16801 tggaataata aagcaccaga actttagcca tttctaacca ctcctcgtgt tatctctatt
    16861 ccgtagcgat tcgctaccac ttatttaaag ataagacgtc cttttcagag tgcaaatttc
    16921 acaaccactt atttgattta taacaacttt cacaagcacg taattttgtc gcaaaatgac
    16981 acatttttat ctcatcgcgt ttttttaatc ataagagcgg cttatggata attatttgag
    17041 atgatatcta ttctcgctaa gaagctgttg caggatatta ccaaacgcgg gtctgcccgc
    17101 gtcagttcac taagcttagt cccacgtagc gaaaatatgg cagccgccat acgccgcgtt
    17161 aattctatgc aatatgatgt ctatacccag acggaggtca gtaatggcaa accatcgagg
    17221 cggttccggc aattttgcag aagaccgcga aagagcatca gaagcaggta aaaaaggtgg
    17281 acagcacagc gggggtaatt tcaagaatga cccgcagcgc gcatctgaag caggtaaaaa
    17341 aggtggcaag agcagtcacg gcaaaagcga caactagccg ggctaatcaa tgacgaatgc
    17401 atttttttct taggctcgtc aaaaagtcat caccgccggt aatcccggcg gttttctgtt
    17461 tatggctcct gatgaacaac ttctggcggt ggaacgtcaa ccaactttct gcttaacaac
    17521 gcattgagta aaatcgcgcc aaaggttgct gtaccaatcc ctcccaacgt aaaaccgccc
    17581 agcgtgagag caaaatcacc cgcgcccagc actaaggtta ctgcgaccat aatcaaatta
    17641 ccgttctggc ttaaatcgac acggttttgt acccatatcc ttgcgcctgc gacggcaatc
    17701 agcccgaaca caacaattga tgcaccacca atcaccgcgg ccggaatggt atgaatcagc
    17761 gcgccaaatt tcggtgaaaa gcccaacagc atggcgatga cggcagcagc gacaaacacc
    17821 agcgtcgagt agacttttgt cacggccatt acaccgatat tctcggcgta ggtggttacg
    17881 ccgctgccac cgacagaacc agaaagcatc gtcgccagcc catcaccgac gaatgcccgc
    17941 cccatatacg ggtccatatt gcgtccggtc atcccggcga ccgccttgag atgacctaag
    18001 ttttccgcca ccagaatcac cgccacgggc gcaatcagca tcattgcctg actattaaaa
    18061 gcaggagtgg aaaaatgtgg caaaccgaac caggcagcat ggctgacgag agtaaaatcg
    18121 acggcttttc ccagccctaa aaggttggtc atcacgccat acagcagaca ggcgacaatt
    18181 aaacctacga gaatcaataa ccgctggatc atgccacggg taaacaccgc caccagccca
    18241 atacacagca ccgtcattac cgccatccag ctatcaaagg ccgaagccga tacacttttc
    18301 actgcgatag gcgctaagtt caggccaatc gccatcacca ccgcacccgt caccaccggc
    18361 ggcattagtc gttcaatcca gcgtgtacca attttcatca ccaccaggcc aatgacggta
    18421 taaaccagcc cacaggcgat aatcccgccc agcgcaatgc tgatattcgg gttaatgccc
    18481 tgaccgttaa agcccgtcgc ggcaatcacc acgccgacaa aagccgcgct ggaaccgaga
    18541 taactgggga cgcgcccgcc ggtaataaag aaaaacagta acgtgccgat ccccgacatt
    18601 aaaatggaaa gattgggatc cagccccatc agaatcggca ttaacaccgt cgcgccaaac
    18661 atcgccaccg cgtgttgaac gcccattact gccgtctgag caaacggcaa tcgttcatcc
    18721 ggcgcgacca cgccgctctc tgtagaggtc gattttaact gccagtgagg aaaaccgaac
    18781 attgccatca gctgtctcct taaggaggtt aacaagcagg gcgcatcagc gcgtgataac
    18841 tgcgatcgaa ccacaccagc ccgtagggtg tggtgtgacg atgaatcgct tcgatggcgc
    18901 aaaacagaat gtcgtgggtg ccgacgctca ccacctggct gatacggcag tcaaacgaaa
    18961 ccagagcctc ttccagttgc gggcatccgg tcacccccgt ctgccagcgg gcggcggcaa
    19021 agcggtgttc catgggcgtt ttgccgccaa aaaggtttga aagcggctcc tgcccggcgc
    19081 tcagtgtatt tacacacagc gttcgatttt cattgaatac cggccagacg gacgccccac
    19141 gattcaggca caccagtaat gtgggcggcg tgtcggtcac gctgcagacg gcgctggcgg
    19201 tgaacccggc gcgcccggct ggaccgtccg tggtgatgat attgaccgcc gcgcccatac
    19261 aggacatcgc atcgcgaaag gtttgttgat caactatgtt catagtttgc tccttacaac
    19321 agcccgcagg cttcttcaaa ggacagacgt ggcaggcgcg caaaaagttt gctgctatcg
    19381 ccatagccga tattaatcag cagattgctc ttcagcgtgc tgcccgcaaa aaaggcgtcg
    19441 tcaacgtgtt gacggtcaaa acccgacatc gggccggtat ccagtcccag cgcacggcaa
    19501 gcgacgatca gataggccgc ctgcatggaa ctgttgcgaa acgctgtttc ttcggcaagt
    19561 tgtgggctgg aggtaaacca actgcgggca tcgccgtgag gaaacagttg cggtaaccgt
    19621 tcataaaatt cactgtccca ggcgacgata gcggtgacgg gcgcggtcag ggttttttgc
    19681 agattgccgc tggaaagtgc cgggcgcaga cgttcttttc cttctgccgt gcggataaac
    19741 acgatccgtg ccggagaaca gttagctgat gtcggccccc atttcatcag ggcataaatc
    19801 tcccgtaacg tctcatcgct gacgggtgtc tcccgccagc cgttgtgagt gcgggcatcg
    19861 gtgaacaagg tgctaagcgc acctgggcta acggcttcgt tcatagcaat tccttacagg
    19921 gcggcttcac ggtgatgtaa caggctggca agcccgttga gtaacagagc attaaacgtt
    19981 tcgggatcgg tcacgttgca ggcgtgtccg ccatagggca tcaccatttt ctggctatcg
    20041 ggcagggcgg catgaagttc actggaacat gctgttggca ccagcagatc atcactggcg
    20101 cagatgattt gcaccgggca gcggatgcga tccgcatggt gactaaagtc agcgcgtttg
    20161 agggcgttaa gtcgacgcag taaattattt ttgccctgaa aatgcgccag tgccagcgcg
    20221 tcttctgcct ccaggcgagg tacgcgggcc gccatccagt cggcgggata gaggaacaac
    20281 ggctgcgctt ccacccatgc ctgcgcgccg ccgctataca gtaatcgttc gcgaacctga
    20341 aaacagcggc gcgtatgggc gtttattcgt agccagccgt taacgctgat cagcacagtt
    20401 accgacgcgg gataatccag cgccagctgc attcccacca gcgcaccgag cgcatggccg
    20461 accaccgcgt aatgctcaat ccctgcggct accagcgcct gatgcagttc cgctgccatc
    20521 tgggcgatac tgtaatcttc tgccagtgtg tcgggattat tgccggtgcc gcgctggtcg
    20581 taacagacta cctgatactc ctgctccagc accgccagtt gcggtaacca gtaactgccg
    20641 ctaccgccaa gacccgaaat caacaccact acgggcgcat cagcataagg gggaggtgag
    20701 agtgaaagtt tcatcgcggc ctcacttggc gatatgcgca attgtggcga tttccaccag
    20761 cgcgtcaggt tttaccagtc cgcactgaat gcagaatcgc gccggtttat cacccggaaa
    20821 aaactcggcg tagatttcgt taatcgcggc gtaatttttc cagtcggtaa taaagatgct
    20881 gttgaaggtc acatccgcca tcgtgccacc cgccgtctcg atcaccttgc ggatagtttc
    20941 cagaacgtgg cgggtttgcg cctttgggtc atcggcaaac agcacgttgt tatgttgatc
    21001 aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg gaacgaaggg
    21061 ggccagcggt gcgctgctgc cagcgggaat aattacggat tttggcatcg ttaaactcct
    21121 taagcgatac gagcaaagga tgtgggagaa agcgcgtcgc agaacgtttc gacgtcgctg
    21181 acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa ttccggcccc
    21241 gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa gccatcgcgt
    21301 agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg gcgtattcca
    21361 cggctgcgca aaatgctgtc cagcggtgta ttgaagaaac cgctgtagcg cggcttcggc
    21421 agcacaatat cgccaggctg cggcaccagt tcatccacca gttgataatc ccaggagcct
    21481 ttcgccagca atttcccctg cagctgcggc tgcttacgca tggttttcag ggcgttcgat
    21541 ttatgaaaat tcggtgagcc gggtccgcca gcttcgacat actgttcatc ccagccattt
    21601 tgaaaccaga tgatcagcat ccctgccgct cgcgctgcgg tcacggcggt ttgaatgttg
    21661 gcaatgaccg ggcgagtggt tgagacatca aacccggcga gatctaagta gccgcctggc
    21721 gtggcataag cgttttgcat atccaccacg atcagcgcac tttgctgcgg atcgaaggta
    21781 atggcttccg gtcgagcggt taaggttgtc atcatgccac ctcctgagtc agcgcaggga
    21841 gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaaggtttcg attcccgaca
    21901 gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg acttcatcta
    21961 acatgcgcgc gacactggcg taagaaccga ctaatgtccc catattgatg tttaccgccg
    22021 aagtgggatc ggccatctga cggacgttgg tgtcagtacc ggagcgggta tctttctgac
    22081 tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag tgttcccatt
    22141 tggcgcgagc ggcatcgtcg gtttcatcgg caatcaccat aaacaacaca taagagccaa
    22201 cgtcgcgccc ggtttgctct gcggcctgtt tcatccgcac agcggtcggg gcgaaagccg
    22261 tgggtgtatt tacgcctttg ccgaaacaga agttgaaatc ggcatactga gcggagaacg
    22321 ccatgccagc gtcgctttgc ccggcgcaga tcactttcat ggggacactc ggttgcggac
    22381 tgacgcgaca atcattcatg gtgaaaaaat cgcctttaaa atcgcttttc cccgtgcccc
    22441 acaggtcgcg cagcacctga acatattcgg tgagatagtc gtaacgacgg gagaaatagt
    22501 catcgccagg ccagataccc atctgctcat actcgggctt ttgccagcca gtcacgaggt
    22561 tgacgccaaa acgcccgcca gagatggagt cgatggttgc ggccatacgg gcgacgattg
    22621 ccggaggtaa cgttaaggtg gcagcagtgg cgtaaatctg aatgcgcgag gtcacggccg
    22681 ccagccccgc catcaaggtg aacgactcaa ggttgtgatc ccagaactca gttttgccgc
    22741 caaagccacg cagtttgatc atcgacaggg cgaaatcgaa atggtagtgc tccgcttttt
    22801 gcacaatggc tttattcagt tcaaaggtcg gcatgtactg cggcgcgtgg gtcgaaatga
    22861 gccagccgtt gttgccaata ggtacgaata cgccaatttt catcatcaac ctctcttcgt
    22921 ctcgtaaagt gaaagtcaga cggggcgctg catcctgcat atccttttca gccgcgtatt
    22981 ggcttgtttg caaagcggat gccagttttt aaaaagttaa tgttgttaat atgttaacaa
    23041 cgcgttatct aaaatatctg gtaaaaagtg gactaaacgg tcaaaacagt tgcacataaa
    23101 acatgcatct gtgcgcgatg agagtgcaga aggtcgaggc cgggcggggg gtttgctatc
    23161 ctgttgccaa tctacaagag gggagagcgc atgacgcaag gcgcagtgaa aacaacgggt
    23221 aaacgttcgc gcgcagtaag cgcgaagaaa aaagcgattc ttagcgcagc actggacact
    23281 ttttcacaat tcggttttca cggcacaagg ctggagcaga tcgcagagtt ggcgggtgtt
    23341 tcaaaaacca atctgctgta ttactttccg tcaaaagagg cgctgtatat tgccgtgctg
    23401 cggcagattc tcgatatctg gctggcaccg ttaaaagcgt ttcgagaaga tttcgccccg
    23461 ctggcggcga tcaaagagta catccgtctg aagctggaag tctcccgcga ttatccgcag
    23521 gcttcgcgcc tgttctgtat ggagatgctg gcaggcgcgc cgctgttaat ggatgaactg
    23581 acgggcgatt tgaaggcatt aattgatgag aaatcggcgc tgattgccgg ttgggtcaaa
    23641 agcggcaaac tcgcgccgat tgatccgcag catttgattt ttatgatttg ggcttccact
    23701 caacattacg ccgattttgc ccctcaggtg gaggcggtga caggcgcgac gttgcgcgat
    23761 gaggtatttt tcaatcaaac ggttgaaaac gtgcagcgga ttattattga ggggattcga
    23821 ccacgttaaa gatgccggag gaggttgtaa catcctccgg ctacctgttt aaccgatcgt
    23881 cattaaactg gcgttaccgc cagcggcagc ggtattcaca ctcagcgaac gttcgatata
    23941 cagccgttcc agaaggatat tgctttcgcc acgggcaaaa ccctgcaccg aaacaattgc
    24001 gccatcccgc gcagcaacgg cttcacacaa tgcgcgaagc tgatccgaat caccgtggaa
    24061 gatcaccgca tcaaagggtt gagcggttat attttccgct ttcgccagtt gaatacgttc
    24121 gctgactgcc gatggcaatg cctttactag ctgacgatgc agcgcgtcat ccggccacag
    24181 tacctggctg cccaccgcca gcacggcggc gagctgagtc agcgcatcct gctcatcatc
    24241 ggcaatacac aacacgcgct cacgcggcag cagcgtccag gtgttgcgtt cacccgtcgg
    24301 ccccggcagc aatcgttgtg ttcctgcctg cgccagctcg ccatattgcg tacataacgc
    24361 ctgcaattct ggacgatttg ctgcccattc ccgcagtgca tttagcggct gagtcaatgc
    24421 ggctttcaac tgtgcatcga ccggatactc tgcatcctga cgcgcgagcg tcactgccag
    24481 cgcactttcc gggcgattcg ccagcagacg gtagagatag agcggaccgc ctgctttcgg
    24541 cccggtaccg gacaaccctt cgccgccgaa tggctgcaca cccaccactg cgcccaccat
    24601 attacggtta acatacaggt taccaacatg ggccgagcca gtgacctggg cgatggtttc
    24661 atcaatacgc gtatggacac caagcgtcag accatagccg gaagcgttaa tctgctcgat
    24721 cagctctggt agctggttac ggttgtaacg caccacatgc agcaccggac caaagacctc
    24781 tttttgcaat tcggcaaagt catccagttc gatcagcgtc ggggcgacaa aggtgccgct
    24841 ttgccattca cgggcatctt cgctgttttc ccgcaccgcc tggaacaccg gacggccttt
    24901 gctacgcatg gtctgaatat ggcgctcaat attggctttc gcttcgctat caatcaccgg
    24961 accgatatcg gtggtcaggc gacccggatt acccatccgg cattcggcca ttgcgccgcg
    25021 cagcattttc aacgtgtggt cggcaatctc atcttgcagg cacatcacgc gcagcgccga
    25081 acaacgctga cccgcactgt cgaacgccga ggccagtaca tccacgacga cctgttcggt
    25141 cagtgctgaa gaatcgacaa tcatcgcgtt catgccgccg gtttcagcga tgagcggaat
    25201 agggcgaccc tgagcgtcca ggcggctggc gatattgcgc tgcagtaacg tagcgacttc
    25261 ggttgaaccg gtaaacatca ccccgcgtac gcgatcatca cccgtcagtt gcgcgcccac
    25321 ggtttccccc tgacctggca gcaactgcac cacgcctggc ggtacacccg cttccagcaa
    25381 aatggcgatc ccttgcgcgg caatcagcgg cgtttgttct gccggttttg ccagcacgct
    25441 gttacctgcc gccagtgcgg cagcaatctg cccggtgaaa atagccagcg ggaagttcca
    25501 cggactgata cacaccacag gccctaatgg acggtgggtt tcgttagcga aatcatcccg
    25561 cacctgtccg gcatagtagt ggagaaaatc gaccgcttcg cgcacttcgg caatggcgtt
    25621 actgaaggtt tttccggctt cacgcaccag aataccaatc agttgctgca tctggctttc
    25681 cattagcacg gcagcgcgat gcaaaatcgc cgcgcgttca accggaggag tggcaaacca
    25741 gattggcgcg ttattaaccg cactttccag cgcctgttct acctcacgcg gcgtggcttc
    25801 acgcacaaag cccacaatat ctttcggttc cgcagggtta ataacgggcg acatctcacc
    25861 tgccgctacc ggttgttcca gcattggcaa ggcctgccat ttttgcagtg cactattgag
    25921 cagggcagaa gagagcgagg ccagccggtg ttcgttagcg agatccagcc ctgccgagtt
    25981 gtcgcgcccg tgaccgtaaa gatcgcgcgg caggggaatt ttcggatgcg gtaatccagt
    26041 ttgcccttcc tgttgcgcca gtttttctac agcagtgacc ggatcggcga ccagttcatc
    26101 cagtggcaaa gaggtgtcgg caatacggtt aacaaacgag gtgttagcac cgttttccag
    26161 caggcgacgc accagatacg ccaacagcgt ttcatgggta ccgaccggag cataaatacg
    26221 acacggacgg ttaagtttgc cgtcggcaac tttcccggtg acctgctcat acagtggctc
    26281 gcccatacca tgcaggcact ggaactcgta ctgacccgga tagtagttct gacctgccag
    26341 ttgataaatc gctgccagcg tatgggcgtt gtgcgtcgcg aactgcgggt agattagatt
    26401 cggcaccgcc agcagctttt tcgcacaggc gagatatgaa acgtcggtat acaccttgcg
    26461 ggtataaacc ggatagcctt caaggccgtc catctgcgca cgcttgattt cactatccca
    26521 gtacgcgcct ttcaccaggc gaatcatcag acggcgacgg ctgcgggtgg cgagatcaat
    26581 caggtaatca atcaccagtg ggcagcgttt ttgataagcc tgaataacaa aaccgatacc
    26641 gttccagcct gccagctcag gctcgaaaca gagtttttcc agcagatcga gggagatctc
    26701 cagacgatcg gcctcttcgg cgtcaatgtt gataccaata tcgtactgac gcgccagcag
    26761 ggtgagtgat ttcagacgtg ggtaaagctc ttccattacc cggtcatact gggcgcggct
    26821 gtaacgcgga tgcagcgccg acagcttgat tgaaatgccc ggaccttcat agatgccacg
    26881 accattggac gctttaccaa tggcgtgaat cgcctgctga taggaaacca tatacgcctg
    26941 tgcatctgcg gcagtcagtg cagcttcgcc aagcatatcg taagagtaac ggaaaccttt
    27001 ctcttccagc ttgcgggcat tggctaacgc ttctgcaatg gtttcacccg tgacgaactg
    27061 ctcgcccatc aggcgcatcg ccatatccac acctttgcgg atcagcggtt caccgctctt
    27121 accgataatg cggttcagcg agcgggagag gctggcttcg ttatgggtgg aaaccagttt
    27181 gccggtaaac agcagccccc aggtagcggc attaacaaac agggacgggc tacgaccaat
    27241 gtgtgactgc cagttaccgt tgctgatttt gtcgcgaatt aatgcatccc gggtggcttt
    27301 gtcgggaata cgcaacaacg cttctgccag acacatcagc gccacacctt cctgcgatga
    27361 cagcgaaaat tcctgcaata acccctgaac catgcctgcg cgaccactgg catttttttg
    27421 attacgcagt ttatcggcca gttgatacgc cagtttgtgc gcctgttcag caactggctg
    27481 cggcaggcgg gcttgttcca gcagcataga aaccgcttcg gtttccgggc ggcgataggc
    27541 cgctgtgatc gcggcgcggg aaaccgactg gggcaatatt tgctcggcaa agtcgaggaa
    27601 tggctggtgt ggttcctctg ccggagtcgg tgcttcatcg ctctcattgg ccgcgccaga
    27661 aagcagcgca ggtagctccg gcagagtatc gctgttttcc agttgttcaa gataagaaaa
    27721 aatcgcctgc ttaattaacc agtgtggtgt gcgatcgata cgtgtcgcgg cagacttaat
    27781 acgctcacgc gtcgcgtcgt ccagcttaac ccccatggtg gtggttccca tgccattact
    27841 cctgttgttc tgaaaggtgc aacttaacgt tatcgtgaaa tatccatgat gttgcaactt
    27901 tgtgcaacca tgttaaatgt gacctgcgta gcaagcttaa aaatgaatga aatgttaata
    27961 aaagaaatcg atatgacagg gattaaaaaa taactcagac tttttctctg cggcagttaa
    28021 catttttgaa aggtgcaacc gcaaaaaatg tgagagagtg caacctgatg aaaaatagtg
    28081 tcgctgagca ctaaaattta atgtaaatgg tgtgttaaat cgattgtgaa taaccagcgc
    28141 ttccggcagg atacggtcgc cctggtaaaa cataaactct gttaccccgt tccggtggca
    28201 gatataacgg caagtttcga cattgccgat aataattttt tggagacttt agatggctat
    28261 tagcacaccg atgttggtga cattttgtgt ctatatcttt ggcatgatat tgattgggtt
    28321 tatcgcttgg cgatcaacga aaaactttga cgactatatt ctgggcggtc gtagtcttgg
    28381 gccattcgtg acggcattat cggcgggtgc gtcggatatg agcggctggc tgttaatggg
    28441 gttgccgggc gcggtttttc tttccgggat ttccgaaagc tggatcgcca ttggcctgac
    28501 attaggcgcg tggatcaact ggaagctggt ggccgggcgg ttgcgtgtgc ataccgaata
    28561 caacaataac gccttaacat tgccggatta tttcaccggg cgctttgaag ataaaagccg
    28621 cattttgcgc attatctctg cgctggttat tttgctgttc ttcaccattt attgcgcttc
    28681 gggcattgtg gcaggcgcgc gtctgtttga aagtaccttt ggcatgagct acgaaacggc
    28741 tctgtgggcg ggcgctgcgg cgacgatcct ttacaccttt attggcggtt tcctcgcggt
    28801 gagctggact gacactgtac aggccagcct gatgattttt gccctgatcc tgacgccggt
    28861 tatcgtcatt atcagtgtcg gtggctttgg tgactcgctg gaagtgatca aacaaaagag
    28921 catcgaaaac gtggatatgc tcaaagggct gaactttgtt gccattatct cattgatggg
    28981 ttgggggctg ggttacttcg gtcagcctca tattctggcg cgttttatgg cggcggattc
    29041 tcaccacagc attgtccatg cgcgtcgtat cagtatgacc tggatgatcc tctgcctggc
    29101 aggggcggtg gctgtcggct tctttgggat tgcttacttt aatgagcacc cggcggtagc
    29161 aggtgcggta aaccagaacg ctgaacgtgt gtttatcgaa ctggcgcaaa ttctgtttaa
    29221 cccgtggatt gccgggattc tgctgtcggc aattctggcg gcggtaatgt caacgttaag
    29281 ctgccagctg ctggtgtgct ccagtgcgat taccgaagat ttatacaaag cgtttctgcg
    29341 taaacatgcc agccagaaag agctggtgtg ggtagggcgt gtgatggtgc tggtggtggc
    29401 gctggtggcg attgcgctgg cggcaaaccc ggaaaaccgc gtgctgggct tagtgagcta
    29461 cgcgtgggca ggctttggcg cggcgtttgg tccggtggtg ctgttctcgg tgatgtggtc
    29521 acgcatgacg cgcaacggtg cgctggcggg gatgatcatc ggtgcgctga cggttatcgt
    29581 ctggaagcag ttcggctggc tgggactcta cgaaattatt ccgggcttta tcttcggcag
    29641 tattgggatt gtagtgttta gtttgctggg taaagcgccg tcagcggcga tgcaaaaacg
    29701 ctttgccgag gccgatgcgc actatcattc ggctccgccg tcacggttgc aggaaagcta
    29761 agggacttag cctgcggcgg ttttgtttgg cttcagcagc gggttgcgct cccttaatgt
    29821 gcctcgccat ataaattgaa tggtgcaggg agcgcgcagg gggcggccaa tcgccgccgc
    29881 cccctgctgt cccggccttc ggggaacgct tcagcgattt tgacgccacc aacacccgag
    29941 ctgttattat gttccgggca aaaagttaga tttgataatc gcggatggac gaaattgctt
    30001 gatacacccg cttatcagtt ttacatggaa gctctgatgc attgagtctg gacagttttg
    30061 tcggctggat acggcgttta cgcggcatcc ggcaagaaca catggttctt tgcaaacaat
    30121 cccatctttc taccctggaa taatcgttta tatcccttgg cattacctct ctttgtttac
    30181 attccaacat cattttataa acattccgct tgtatttttc tttgccgtaa tgataatcgc
    30241 tatcactgcg atttactttt ctttgcatag attgactcag aaaaacgttt aagggtgggt
    30301 ggcatgtttg ttccgtttct cattatgttg cgcgaaggac ttgaagccgc gctgattgtc
    30361 agtttgattg ccagctatct taagcgtacc cagcgaggcc gatggattgg tgtgatgtgg
    30421 attggcgtgt tgcttgccgc tgcgttgtgc ctgggcctgg gtatcttcat taacgaaacc
    30481 accggcgaat ttccgcaaaa agaacaggaa ctgtttgaag gtatcgtggc ggtgatcgcc
    30541 gtggtgatcc ttacctggat ggttttctgg atgcgcaaag tgtcgcgcaa cgtcaaagtg
    30601 caactggaac aggcagtcga tagcgcattg cagcgtggaa atcatcatgg ctgggcgctg
    30661 gtgatgatgg tcttttttgc cgttgcaagg gaagggctgg agtcggtctt tttcctgctg
    30721 gcggcatttc aacaagatgt cgggatctgg ccgccgctgg gtgcaatgct cggtcttgct
    30781 actgccgtgg tgctcggctt cctgctctac tggggcggta ttcgcctcaa tcttggtgca
    30841 ttttttaaat ggaccagcct gtttattctc ttcgtcgccg cagggctggc ggctggtgcc
    30901 attcgcgcat ttcatgaagc cggattgtgg aaccactttc aggaaatcgc cttcgatatg
    30961 agcgcggtcc tctcaactca ctcgctgttt ggcacgctga tggaagggat ttttggctat
    31021 caggaagcac cgagcgtcag cgaagtcgcc gtctggttta tttatctcat cccggcgctg
    31081 gtggcatttg ccctgccacc acgcgcaggg gcgacagcgt ctcgctccgc gtagcaaata
    31141 cgacgcaaac tcttgcttag ttacaacata ctttaaaggg atagtctcgt catgaccatt
    31201 aacttccgcc gtaacgcatt gcagttgagc gtggctgcgc tgttctcttc tgcttttatg
    31261 gctaacgccg ctaatgtgcc gcaggtcaag gtgaccgtga ccgataagca gtgcgaaccg
    31321 atgaccatta cggttaacgc cgggaaaaca cagttcatta ttcagaacca cagccagaag
    31381 gcgctggagt gggagatcct caaaggcgtg atggtggtgg aagagcggga aaatatcgcc
    31441 cctggcttta gccagaaaat gacggcgaat ttacagcctg gcgaatacga tatgacctgc
    31501 ggtctgctga ctaacccgaa agggaagttg atcgtcaaag gtgaggcaac ggcggatgcg
    31561 gcgcaaagtg atgcgctgtt aagtcttggt ggtgcaatta ctgcatataa agcgtatgtc
    31621 atggcggaaa ccacgcagct ggtgaccgac acgaaagcct ttactgacgc tattaaagca
    31681 ggcgatatcg aaaaagcgaa agcactgtat gcgccgacgc gccagcacta tgagcgcatc
    31741 gaaccgattg ctgaactgtt ctccgatctg gatggcagca ttgacgcccg tgaagatgat
    31801 tacgagcaaa aagccgccga tccgaaattc accggtttcc accgtctgga aaaagcattg
    31861 tttggcgaca acaccaccaa aggcatggat cagtacgctg accagcttta taccgatgtg
    31921 gtcgatttgc aaaaacgcat cagtgaactg gctttcccac cttcaaaagt ggtcggcggt
    31981 gcagccggac tgattgagga agtggcagcc agcaaaatca gcggtgaaga agatcgctac
    32041 agccacaccg atctgtggga tttccaggct aacgttgaag gctcgcagaa aattgtcgat
    32101 ctgctgcgtc cacaactgca aaaagccaac ccggaactgt tggcaaaagt cgatgccaac
    32161 tttaaaaagg tcgataccat tctggcgaaa taccgtacta aagacggttt tgaaacctac
    32221 gacaaattga ccgatgccga ccggaatgca ctgaaaggac cgattactgc gctggcggaa
    32281 gatctggcgc aacttcgcgg tgtgctggga ttggattaag cattatgcag tatgaagata
    32341 aaaacggcgt gaatgaaccg tcacgccgac gtttactgaa aggcattggt gcgctggcgc
    32401 tggcgggaag ttgtccggtc gctcatgcac aaaaaacgca aagtgcgccg ggtacgcttt
    32461 caccggatgc acgcaatgag aaacagccgt tttatggtga gcatcaggca gggatcctga
    32521 cgccacaaca ggccgcaatg atgctggtgg cgtttgatgt gcttgccagc gataaacccg
    32581 atcttgaacg gttgtttcgc ttgttgactc agcgttttgc ttttctgact cagggcggag
    32641 cggcaccaga aacgccaaat ccgcgcctgc caccactcga ttccggcatt cttggcggct
    32701 acattgcgcc cgataatctc accatcacgt tatcggtggg tcactcattg tttgatgagc
    32761 gctttggcct tgcgccacag atgccgaaaa agttgcagaa gatgacgcgt tttcccaacg
    32821 actcgctgga tgcggcgtta tgtcatggtg atgtgttgct acaaatttgc gccaacaccc
    32881 aggacacggt gatccatgcg ctgcgcgata tcatcaaaca tacgccagat ttgctcagcg
    32941 tgcgctggaa gcgggaaggg tttatttccg atcacgcggc gcgtagtaaa ggcaaagaga
    33001 cgccgattaa tttgctgggt ttcaaagacg gcactgccaa tcccgatagc cagaatgata
    33061 agttgatgca aaaagtggtg tgggtaacgg cagatcagca ggagccagcg tggacaatcg
    33121 gtggcagcta tcaggcagta cgcttgattc agtttcgagt ggaattttgg gacagaactc
    33181 cgctgaaaga acagcagacg atttttggtc gtgataagca aaccggtgcg ccgctgggaa
    33241 tgcagcatga gcatgatgtt cccgattacg ccagcgatcc ggaagggaag gtgattgcgc
    33301 tggacagcca tatccggctg gcgaatcccc gcacggcgga gagtgagtcc agcctgatgc
    33361 tgcgtcgtgg ctacagttat tcactgggcg tcaccaactc cgggcaactg gatatggggc
    33421 tactgtttgt ctgttaccaa cacgatctgg aaaaaggctt cctgacagta caaaaaaggc
    33481 tcaatggcga agcgctggag gaatacgtta aacctatcgg cggcggttat ttttttgcgc
    33541 tgccgggagt gaaggacgcg aacgattatc tcggaagcgc gttattgcgg gtttaatgtt
    33601 tttagacgga taaggcgttc ttgcgcagat gcctgatgcg atgcttgcgc gtcttcttat
    33661 gcctacaatc ggtgcgggtt cggtaggcgg ataaggcgtt cacgccgcat acggcgatcg
    33721 tgcacagatg cctgatgcaa atcctgctga aagcacacag cttttttcat ctctgtcatc
    33781 actctgtcat ctttccagta gaaactaatg tcactaaaat ggtgttttat agttaaatat
    33841 aagtgaatat attgttgcaa tgaatgcgag atctgttgta cttattaagt agcagaggaa
    33901 gttcccggca gtgatagtca gtcactatgg agatcgcgga tggtaacgtc ctgtactgga
    33961 caatgttttg ttaagcaacg ctccaccact cgcggagatt tctcctccgg tagcaacttc
    34021 gtcactctgt atttttctca cttcctctct ttactcgtgt taccgacgcc gcaaacggtg
    34081 tgcgtagccg tttttcggca ttttatccaa aagcaaacta tggcttacaa ggaagccaac
    34141 cctctgatgt tcgtgcgcat aatcgcgctg ccaacggcgc gtgtgatgaa tacaaacaac
    34201 tcaaggtgct ctccatggga agacaaaaag cagtgatcaa agctcgtcgt gaggcaaaac
    34261 gtgtgctgag acgggattcg cgcagccata agcagcgtga agaagaatcg gtcacctcgc
    34321 ttgtgcagat gagcggcgta gaagccattg gtatggctcg cgacagtcgc gatacctcgc
    34381 ccatcctcgc gcgaaatgaa gcgcaattgc actatctgaa ggctattgag agtaagcagt
    34441 tgatattcgc cacgggcgaa gccgggtgcg gaaaaacctg gatcagtgca gcaaaagcgg
    34501 cagaggccct gatacataag gatgtcgaca ggattatcgt cacccgtcca gttctgcaag
    34561 ccgatgaaga tcttggcttc ttacctggag atatcgcaga aaagtttgct ccctattttc
    34621 gcccggtcta cgacgtgctg gtccggcgct taggggcttc ctttatgcag tactgcctgc
    34681 gaccggaaat agggaaggtg gaaatcgcgc cgttcgccta tatgcgtgga cgtacctttg
    34741 aaaatgcagt cgtcattctt gacgaggcgc agaatgtgac tgccgcgcaa atgaaaatgt
    34801 ttttaacccg cctcggggag aacgtgacgg ttatcgtcaa cggtgatatc acacaatgcg
    34861 atttacctcg cggtgtgcgc tccggattaa gtgacgcgct ggaacgtttc gaagaagatg
    34921 aaatggtcgg gatcatccga ttcggtaaag aggattgcgt acgttcggca ctttgccaac
    34981 gtacgctgca tgcatacagt taagtgtatt atcggtgcag agcccgggcg aaccgggctt
    35041 tgttttgggt gtttatgccc ggactagcgc tttttctgaa acaaccattt ttatttgccc
    35101 ctggctggtg aagtgtacgc tcatcctgtg gcttttttgt agttgctgat agagctcatc
    35161 aggtattgct aagctctctg catattcttg tggcgtatat tggtaggctg catgatgctg
    35221 ctgtttttga aaacgcagct tattgtacag cgcccagaca attaacacga cggcattcgc
    35281 cactgccagc aaaaaataga actgaagtcg gcttctggcc tcgctttgcc agtaataacc
    35341 cgtcagcaga tccatggcga ataagaatat gaacaaagca aataatgtcc acaagatggt
    35401 tgtggcaaca taatcaacca gtaaacgtac tggcgattgt cgggtcgtaa taattaaatt
    35461 gttcatatta acctctcaga atcccgcgat cgggacttac ccaacgggcg cgttgctttt
    35521 taggcatcaa catgactcgt gtaaatgata ccaatgtcgt tgccaggctc agcatccaga
    35581 aaataaccgg gaaccaaata atccagaaaa gcgatgaagt cagattatgc tcatagcgat
    35641 tctcgatcat caggctgaca ataaattgca gtaaacataa cgtacacaat aatattccgg
    35701 cagtatgtgt cgcagcgata tgtgtcaatt caatatttaa cggtacaccg gcaagttgga
    35761 ctgcgtaaat aatgaaaccg accaggcagg taaaggccca tattgtcgtc agacagtatt
    35821 caaaaaacag tggccacatt cgaaagtttt ctttgcgcca tagccttgtc atatttttga
    35881 ggaatacttc tgcaccgccc tgagcccagc gcaggcgctg tttccacagc ccttttaacg
    35941 tttcaggcat taatatccag cacagcgccc gtggctcgta aaaaatcgtc cactgattca
    36001 actgcagctt ccagctaata tcaatatctt cggtgatcat atcgtcactc cagtaaccca
    36061 cttctgccag ggcgctgcga cgaaatgcgg caataacacc ggaaacggta aatacgtttc
    36121 catagatacg ctgggttcgc ttgatcaaac caataattga ggaatactcg ccaacctgaa
    36181 ttttacccac cagggtagaa cgtgttcgaa tacgaggatt accggttacg gcacccacac
    36241 gcgggttgta caacatcggt tccacaatat atgccgccgc atcgcggtct aataacgcat
    36301 cgccatcaat gcacaccaga tattcacttt tcgccgcggc agctccggtt ttaagcgcaa
    36361 tggctttccc ctggttttgc gccagatgaa tgacccgcaa atggggaatt tgtgcagcca
    36421 tgcgatccag gatggcacgg gttctatctg ttgaaccgtc atttacggca ataacttcaa
    36481 tgttctcata acgctgtgct aaagcggcgt gtatggtttc ctctacgttt ttctcctcat
    36541 taaaacaggg aatgataatg gagatagacg gattatcttt caactgggga gctggtgcgt
    36601 tttctcccca cggccagtgg cgttcacgat agacccagaa atagacgccg ccaacaatcc
    36661 acataatgga cataaaaaac ggccagaaga aaacgaacct catcatcagt tcaccagagt
    36721 gaaagtacgc tacgcatagg gggatgcata gcaccagaca taatataaag aacgatacga
    36781 tgcgattaat catttttcgg ataccaggct gttgaaaact caggacgaat aacgtctatt
    36841 tcaggttggt catgcagaaa attgtcggga taataaccat agtttttcac tccattcagt
    36901 tgtaataggc tcatccagtg agcgagttgt tgcgaagaaa tagcctgatg ctgaccattt
    36961 ttctgccagt tttgtgcctg taattctaaa atagatttgt ctttagcctg agggatgttt
    37021 ttaatttgat tggtcaattg tattaaccat tggtcagccg atttttctgc gacaccttcc
    37081 atataaggca tagccataat agcggtccag tcatagcttt ttaggaaatc agcataattc
    37141 tgtgcaaacc aggcttcact ttcaggttgt attaccggaa gtgcaaaaat atttcgtgca
    37201 gttttaacat gtggaccgcg aatggctttt acgcgcgcac taagttctaa agtgaagtca
    37261 gttaacgcac gacttttaaa gcgggtccac tgtttaaatt gctccgggtt ttgtcgaatt
    37321 tcgctcagac tcccgctaaa gcctgcttgc tgataggccg tgatagccgg tgcactggca
    37381 tcttcataat ctgaaagcaa agcatcatcg tggaacaata ggccatcaaa agcagcatgt
    37441 ccggcaagat cttcatataa catgccaact tgtgctctga ccttgtcatc aaaaggagag
    37501 agacggcgat attgttctgg atgaatttgt gcttttttct cccctgttgg taaatatttt
    37561 actcgcgtta atgtgggatc taaatcccag cttaataccg gcatccacgc atagatgttt
    37621 acacctgagc gggtacgtaa ttgccaggca acccgactaa aaatatctgc tttcattggt
    37681 agcaaacgat ttggaaacca gacctctttg accagcccat caccatcggg atcagcaaat
    37741 gcctgcaaat acacggttga tatttgcata tctttcaccc gctgaattag cacatcaata
    37801 ttgcgatcca tttgctggcg attttcgtca taaacgtaat caagatcgat atgcattatc
    37861 cgttgtgatg atttttcctg tacggtaata atttgctggg caaactcttt taatgaggga
    37921 ttattggcga ttaatactcg cggaatggaa tccaattgtg acgaatttgc caaacctgat
    37981 tcaagggtga agaacatgtc ataaccgagt ttttttaatt cctctatcgc tatgccattc
    38041 gcttcgccat aaggccaaac aaaaacgtgt ggatttacct tagcctttgt acgcaggtat
    38101 tctgtcattt ttacagcatc cagacgaatt ctttcccggt attctgctgc ggtttcatac
    38161 cgtgcgtggt cagtaaaata tgcacgattt acatatacag gcaataagct gccggtggca
    38221 ttagcctgaa taccgtagtg agaattccat gtatgagaag cgacctcaac gagcggggaa
    38281 cgcgcaactt ctcgcacttg ttgccacgtg gcaaaatatt ctcgatcgac catctcatcg
    38341 ccaaatttta cttgttcatc cgctggcgta tcgacccaac tgccgacggg ggcccataca
    38401 gcaggccatt ggaaggcctg aagaattggg aagacgcggg tataaaaact ctggtagccg
    38461 tcatcaaaag tcagcactac tgctttttcc ggtagcggtt ttcctcctcg atgtgcttca
    38521 cgaatttgag caatactgac aggttgataa ccgttctcgc gcagccaggc aaattgttca
    38581 cgcagtgctg atgtccgcac tgacataaaa cgctggtcgg cagcttcgtc ttcaacgtta
    38641 tgccatgaaa tcgccacaaa accattatgc ggccacggtt gctcggcgag taaagactcg
    38701 cgatcctgtg gcggtataaa tgatgttttt gactggctaa tgcacgcagt gagcataatt
    38761 atactcacca gcatcaggag atatttattt ccattacgta acatatttat ccttaaaatc
    38821 tgaatgtcat atcgaattca acgtataagt tgtgttctct gtcaccgtca taaggtcgtt
    38881 tttcccagcg tagcgttgcg ccagcatcaa tcacgtcatt ccagctaatg cgttgcccgt
    38941 agccgagttg ggtgacgaca tccgtgccat aatgtttttg ccaggaggca ccaacacctg
    39001 cgctgaagat ttgctcccag ctattttcat agcttcgcca taacaaatga cttgcctcaa
    39061 atgccggaac aatatcgaac gtttttatag ggttgtagta tggggtatcg tgttctgtat
    39121 tttgttcgta atacagactg ggtaggaaat cgacaatcaa atatggtgaa gaccagatgc
    39181 gttcctgacc atcaattgag acttcatgac gctggttact gtcggaaaaa tcagtgaaag
    39241 cccaggagac accgtactta cgccgctcat tttgatacca gcgaacataa gcctgagcac
    39301 tgttgcctgt aacaccattt ttcattgccc gtaatggaac gcggtgagag aggcgttcca
    39361 gttgcgaacc aatacgccag ttatcattaa aatcatacca gccagacagg cgcgcgccgg
    39421 gtttatgctc atgattgaaa aagcgttcag cgtactccgc ctcgagccag atattacgtg
    39481 accgccactc aacacccgcc agccagtcgc gaacaatccc ttttccttcg ctaaattgtc
    39541 catcggcata accgaatcca gcaaaaccgc gccagttatc cttcagcggt ggtgaataaa
    39601 cgatggtggt taagtcgaca tcatgtttac cactatccgg gccttcggca tcaattcctg
    39661 ttgagccagc gatacgaagc tctgcaagat tatgtacatc aaccgcacgt tttaatcgta
    39721 caacgccggg atcttgcggt tcacgttcga caacatcgtg cgttaagaca gctgcctgct
    39781 gccattcttg taacgttaat gctgtccagg cttgttcaac ctccagatta atattacgtg
    39841 gctcgatcac ttctgctttt tttaattcat tttctgctgc acgaggccaa ccgcgggctt
    39901 gtaacacact cgcgtaatca atgcgcagtc cctgatttcc tggtgcgtta taagcaagct
    39961 ctctggctgt catttcagcc tgaggaagat cattactata ttttgctacg gttgagagaa
    40021 acgaatgccc ctgtaaccag gtatcattcg ggatgctcgt aggcgtgccc attaaccgaa
    40081 ggaaaggcgg cgaagtatta atggtatgtt gggtgacagt tagcgcgccc ggataatttt
    40141 cactctccag gtggctgtaa aagagatccg caagttcttc atcggataaa tccggggcaa
    40201 tggtctcctt gtgataaaag agctcggtca ttattgactg tgcttttttc ggctgatgat
    40261 ctttgagata agccgatgca acccaatatt gcccccaggg cggaataatt tgccccgtct
    40321 tttttaatcg ctgatagtga gaaataacgt ctttataacg atcgcgagtt aataacgcgc
    40381 caagatgatc aacctgaata cgctggtact gggcagtgcg gtctgggtta tcgtgccaca
    40441 gaatttctaa tgcagcgtat tgggcgaggg cgcgatcggc aatggcataa cgttcacttt
    40501 cactgcgcgt aggcataaac gacagtctga ccagttcggc atgaatatca gcgcgaatat
    40561 ctggcgttaa attggcatcg tcaatcgcgg cagcaagttg attattacgt aatgcctgca
    40621 cgtattctgt gggatattgt tgcgtagatg cattttcagg taatgactct gtcatcgccc
    40681 gtaattcatc ctgatgacgc cccgccagtt tatagatata ggcttctgcg agtaaattgg
    40741 ctttgtccgg tgctccagag ttaagctgct taagtttaac cagcgcagta tcatagtgac
    40801 ccgcatctgc cagggttaaa atttgtcccc gttgataatc cttattttgc ggctccagag
    40861 agagcgcctt ttgccacagt gtaagcgagt tttgccattg ttgcaggtta cgataagcga
    40921 cggcgacagc tgcataacca cgcgctggta attgctgata acggtagcgg ttgtaaacgg
    40981 taataacctg tttatcttgc ccggcccata atgcaatctg taaccagtca gcaatttggt
    41041 tattactgag tgctgatttt tgtgcaaacc ataacaaagc tggctgagta tttcccttgc
    41101 gagcttcaat aatcaatgca tcataggcgt tattaacggc actcttcgcc gcgggactcg
    41161 ctgctaaaaa tgcggcagta agaattttca aagcccattt ggttttcggg cacctttttc
    41221 tgctacttga atacatcctg tattactcca tgtatcgcca aaatctctct ctgtatctaa
    41281 ttacaggtaa ctgaaaagaa agatattttt gcacctcata atccgttatt aaacgcggaa
    41341 gagagacgtg aattgttgat gatgagaaga agaaatgatg agcagagtgt ccatataaaa
    41401 tccttttctc gcccgaaaat ccattccaat gatgaggatc ttcaggaata cggcataaat
    41461 cccaatgcct ttttcaaaat aaattaggat taaaataatt aaatcagtaa
//
LOCUS       DBANUU010000028        40454 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000028 DBANUU010000000
VERSION     DBANUU010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.028"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..110
                     /locus_tag="ACIE68_001426"
     CDS             <1..110
                     /locus_tag="ACIE68_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFX5907770.1"
                     /translation="FLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA"
     gene            89..193
                     /gene="speFL"
                     /locus_tag="ACIE68_001427"
     CDS             89..193
                     /gene="speFL"
                     /locus_tag="ACIE68_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFX5907771.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            504..2702
                     /gene="speF"
                     /locus_tag="ACIE68_001428"
     CDS             504..2702
                     /gene="speF"
                     /locus_tag="ACIE68_001428"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFX5907772.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKETRPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2699..4018
                     /gene="potE"
                     /locus_tag="ACIE68_001429"
     CDS             2699..4018
                     /gene="potE"
                     /locus_tag="ACIE68_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFX5907773.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4074..5714)
                     /gene="pgm"
                     /locus_tag="ACIE68_001430"
     CDS             complement(4074..5714)
                     /gene="pgm"
                     /locus_tag="ACIE68_001430"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFX5907774.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLDRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     TATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5740..6285)
                     /gene="seqA"
                     /locus_tag="ACIE68_001431"
     CDS             complement(5740..6285)
                     /gene="seqA"
                     /locus_tag="ACIE68_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFX5907775.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6470..7234
                     /gene="ybfF"
                     /locus_tag="ACIE68_001432"
     CDS             6470..7234
                     /gene="ybfF"
                     /locus_tag="ACIE68_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFX5907776.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7374..7667
                     /gene="ybfE"
                     /locus_tag="ACIE68_001433"
     CDS             7374..7667
                     /gene="ybfE"
                     /locus_tag="ACIE68_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFX5907777.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7807..8337
                     /gene="fldA"
                     /locus_tag="ACIE68_001434"
     CDS             7807..8337
                     /gene="fldA"
                     /locus_tag="ACIE68_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFX5907778.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8547..8633
                     /gene="uof"
                     /locus_tag="ACIE68_001435"
     CDS             8547..8633
                     /gene="uof"
                     /locus_tag="ACIE68_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFX5907779.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8626..9072
                     /gene="fur"
                     /locus_tag="ACIE68_001436"
     CDS             8626..9072
                     /gene="fur"
                     /locus_tag="ACIE68_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFX5907780.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9157..9483)
                     /gene="chiQ"
                     /locus_tag="ACIE68_001437"
     CDS             complement(9157..9483)
                     /gene="chiQ"
                     /locus_tag="ACIE68_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFX5907781.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9533..10939)
                     /gene="chiP"
                     /locus_tag="ACIE68_001438"
     CDS             complement(9533..10939)
                     /gene="chiP"
                     /locus_tag="ACIE68_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFX5907782.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSPHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(11519..13183)
                     /gene="glnS"
                     /locus_tag="ACIE68_001439"
     CDS             complement(11519..13183)
                     /gene="glnS"
                     /locus_tag="ACIE68_001439"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFX5907783.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13451..15397)
                     /gene="nagE"
                     /locus_tag="ACIE68_001440"
     CDS             complement(13451..15397)
                     /gene="nagE"
                     /locus_tag="ACIE68_001440"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFX5907784.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVRAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            15730..16530
                     /gene="nagB"
                     /locus_tag="ACIE68_001441"
     CDS             15730..16530
                     /gene="nagB"
                     /locus_tag="ACIE68_001441"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFX5907785.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16590..17738
                     /gene="nagA"
                     /locus_tag="ACIE68_001442"
     CDS             16590..17738
                     /gene="nagA"
                     /locus_tag="ACIE68_001442"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFX5907786.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            17747..18967
                     /gene="nagC"
                     /locus_tag="ACIE68_001443"
                     /gene_synonym="nagR"
     CDS             17747..18967
                     /gene="nagC"
                     /locus_tag="ACIE68_001443"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFX5907787.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            19015..19767
                     /gene="nagD"
                     /locus_tag="ACIE68_001444"
     CDS             19015..19767
                     /gene="nagD"
                     /locus_tag="ACIE68_001444"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFX5907788.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(19818..19916)
                     /locus_tag="ACIE68_001445"
                     /pseudo
     CDS             complement(19818..19916)
                     /locus_tag="ACIE68_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20164..21828
                     /gene="asnB"
                     /locus_tag="ACIE68_001446"
     CDS             20164..21828
                     /gene="asnB"
                     /locus_tag="ACIE68_001446"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFX5907789.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVTDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            22207..22283
                     /locus_tag="ACIE68_001447"
     tRNA            22207..22283
                     /locus_tag="ACIE68_001447"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22241..22243,aa:Met,seq:cat)
     gene            22292..22376
                     /locus_tag="ACIE68_001448"
     tRNA            22292..22376
                     /locus_tag="ACIE68_001448"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22326..22328,aa:Leu,seq:tag)
     gene            22400..22474
                     /locus_tag="ACIE68_001449"
     tRNA            22400..22474
                     /locus_tag="ACIE68_001449"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22432..22434,aa:Gln,seq:ttg)
     gene            22490..22566
                     /locus_tag="ACIE68_001450"
     tRNA            22490..22566
                     /locus_tag="ACIE68_001450"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22524..22526,aa:Met,seq:cat)
     gene            22615..22689
                     /locus_tag="ACIE68_001451"
     tRNA            22615..22689
                     /locus_tag="ACIE68_001451"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22647..22649,aa:Gln,seq:ctg)
     gene            complement(22843..24018)
                     /gene="ubiF"
                     /locus_tag="ACIE68_001452"
     CDS             complement(22843..24018)
                     /gene="ubiF"
                     /locus_tag="ACIE68_001452"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFX5907790.1"
                     /translation="MTNQPTEIAIVGVGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHDDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPILKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFMRNLGLMAAERAGVLKRQALKYALGL"
     gene            24164..25588
                     /gene="miaB"
                     /locus_tag="ACIE68_001453"
     CDS             24164..25588
                     /gene="miaB"
                     /locus_tag="ACIE68_001453"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFX5907791.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            25741..26781
                     /gene="ybeZ"
                     /locus_tag="ACIE68_001454"
     CDS             25741..26781
                     /gene="ybeZ"
                     /locus_tag="ACIE68_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFX5907792.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            26778..27245
                     /gene="ybeY"
                     /locus_tag="ACIE68_001455"
     CDS             26778..27245
                     /gene="ybeY"
                     /locus_tag="ACIE68_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFX5907793.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQKESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            27335..28213
                     /gene="corC"
                     /locus_tag="ACIE68_001456"
     CDS             27335..28213
                     /gene="corC"
                     /locus_tag="ACIE68_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFX5907794.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            28238..29776
                     /gene="lnt"
                     /locus_tag="ACIE68_001457"
     CDS             28238..29776
                     /gene="lnt"
                     /locus_tag="ACIE68_001457"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFX5907795.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPSLWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(29854..31731)
                     /locus_tag="ACIE68_001458"
     CDS             complement(29854..31731)
                     /locus_tag="ACIE68_001458"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFX5907796.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSNKPGAWV
                     MALSPDEKILLIPGMVRGDIVRINTITHQKESYPASDARGTISAMRFRPENGDVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            32186..33094
                     /gene="gltI"
                     /locus_tag="ACIE68_001459"
     CDS             32186..33094
                     /gene="gltI"
                     /locus_tag="ACIE68_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFX5907797.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            33264..34004
                     /gene="gltJ"
                     /locus_tag="ACIE68_001460"
     CDS             33264..34004
                     /gene="gltJ"
                     /locus_tag="ACIE68_001460"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFX5907798.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            34004..34678
                     /gene="gltK"
                     /locus_tag="ACIE68_001461"
     CDS             34004..34678
                     /gene="gltK"
                     /locus_tag="ACIE68_001461"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFX5907799.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            34678..35403
                     /gene="gltL"
                     /locus_tag="ACIE68_001462"
     CDS             34678..35403
                     /gene="gltL"
                     /locus_tag="ACIE68_001462"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFX5907800.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            35521..36456
                     /gene="rihA"
                     /locus_tag="ACIE68_001463"
     CDS             35521..36456
                     /gene="rihA"
                     /locus_tag="ACIE68_001463"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFX5907801.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            36540..38210
                     /gene="hscC"
                     /locus_tag="ACIE68_001464"
     CDS             36540..38210
                     /gene="hscC"
                     /locus_tag="ACIE68_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFX5907802.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDEARECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGISGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(38270..39721)
                     /gene="djlC"
                     /locus_tag="ACIE68_001465"
     CDS             complement(38270..39721)
                     /gene="djlC"
                     /locus_tag="ACIE68_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFX5907803.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(39718..40425)
                     /gene="ybeU"
                     /locus_tag="ACIE68_001466"
     CDS             complement(39718..40425)
                     /gene="ybeU"
                     /locus_tag="ACIE68_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFX5907804.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         9947 a        10329 c        10145 g        10033 t
ORIGIN      
        1 gttttttgtt gatcttcttc acagattata gccatttcat ggatagaata actctacctt
       61 caactgacac agcaagaggt aaaggtaaat ggaaaataat aaccgcttaa tgcctcatat
      121 aaggcggaca acccatatca tgaagtttgc ccatcgtaat agttttgact ttcatttctt
      181 taatgcccgc tagtcttctg actaaagggc accccaacgt acaggtctcc ctgactttaa
      241 gcattacagg ttaatacctg tattcctcgg tgctcatata ctgctaaccc ttttaaactc
      301 taaataattc gagtcgcagc acttgcaact tgaggtatga cgagtatagc cagttaccgg
      361 gctggtctgg gttattgcat ctgcaaaaag caaactactg atttatttat cagcggtgga
      421 gctttgcttt ttttccggcg tgatcgattt ctcctttgag aaattgagga cctgctatta
      481 cctgaaataa agagatgaac aaaatgtcag aattaaaaat tgccgttagt cgttcttgcc
      541 cggattgttt ttccactcat cgtgcatgcg tgaatataga cgaaagtaat tatattgacg
      601 ttgccgccat tattttatca gtcagtgatg ttgaacgtgg aaaactcgat gaaatagacg
      661 ctactggcta tgacattcct gtttttattg caacggaaaa tgaagaacgt gtacccgcag
      721 aatatctgcc acgtatttct ggtgtcttcg aacattgcga atcacgtaaa gagttttatg
      781 gacgtcagtt agaaaccgct gccagccatt atgaaacgca actgcgcccg cccttcttcc
      841 gcgcactggt cgattatgtt aatcagggca atagtgcttt tgactgccct ggtcatcagg
      901 ggggtgagtt tttccgtcgc catccggcag gcaatcagtt cgtggaatac tttggcgaaa
      961 tgctcttccg ctctgacttg tgtaatgctg acgtagcaat gggcgatctg ctgattcatg
     1021 aaggtgcgcc atgtattgca caacagcatg cggctaaagt ctttaatgcc gataaaacct
     1081 atttcgtttt aaacggtact tcatcttcta ataaagtggt cttaaacgca ttgctgacgc
     1141 cgggcgatct ggtgttgttt gaccgcaata accataaatc taaccaccac ggtgcgttgc
     1201 tacaggctgg cgcgacaccg gtttatctgg aaaccgcacg taacccgtat ggctttatcg
     1261 gtggcattga tgcgcactgt tttgaagaaa gttacctgcg tgagctgatt acagaagttg
     1321 caccgcagcg ggcaaaagag actcgtcctt tccgcctcgc cgttattcag ctaggcactt
     1381 acgacggtac gatttataac gcccgtcagg tggtggataa aattggtcat ctgtgtgact
     1441 acatcctctt tgactccgca tgggtcggct atgaacagtt tattccgatg atggctgact
     1501 gttcgccgct gttgctggat cttaatgaga acgatccggg tattctggtt acgcaatccg
     1561 tacataaaca gcaggctggt ttttctcaaa cttcacaaat ccataaaaaa gacagccaca
     1621 tcaaagggca acagcgttat gtaccgcaca aacgcatgaa caacgccttt atgatgcacg
     1681 cctccaccag cccgttctat ccgctgtttg ccgcactgga tatcaacgcc aaaatgcatg
     1741 aaggtgtcag cggtcgtaat atgtggatgg attgtgtggt aaatggcatt aatgcccgca
     1801 aactgatcct cgataactgt cagcatattc gtccgttcgt acctgaactg gtggatggta
     1861 aaccctggca gtcgtatgaa acagcgcaaa ttgcggttga tctgcgcttc ttccagtttg
     1921 taccagggga acactggcat tcttttgaag gctatgcaga gaatcaatac tttgtcgatc
     1981 cttgcaaact gttgctgaca accccaggta ttgatgcacg taacggcgaa tatgaagcgt
     2041 tcggtgtacc cgcgacgatt cttgctaact tcctgcgcga aaatggcgta gtgccggaaa
     2101 aatgcgatct taactccatc ctcttcctgc tgactccggc agaagatatg gccaagcttc
     2161 agcaacttgt tgccctgctg gtacgcttcg aaaaactgct tgagtccgac gcgccattag
     2221 cagaagtgct gccttccatc tacaaacagc atgaagagcg ctacgccggt tataccctgc
     2281 gtcagttgtg tcaagaaatg catgatttgt atgcccgtca caacgtgaaa caactgcaaa
     2341 aagagatgtt ccgtaaggaa cacttcccac gcgtaagcat gaatccgcaa gaagccaact
     2401 acgcctattt acgcggtgaa gtggagctgg ttcgtctgcc ggatgcagaa ggccgtatcg
     2461 ctgccgaagg tgcgcttcct tatcctccgg gtgtgctgtg cgttgttccg ggtgaaatct
     2521 ggggtggtgc tgttctgcgt tacttcagcg ctctggaaga agggatcaac ctgttgccag
     2581 gttttgcacc ggagcttcag ggtgtctata tcgaagaaca tgatggtcgt aagcaagttt
     2641 ggtgctatgt catcaagcct cgtgatgcgc aaagcaccct gttgaaaggg gaaaaattat
     2701 gagtcaggct aaatcgaaca aaatgggcgt cgttcagtta accatactga cgatggtcaa
     2761 catgatgggc tccggtatca tcatgctgcc gacaaagctt gccgaagttg ggacaatttc
     2821 aattatctcc tggctggtga cagccgtagg ctcaatggca ctggcatggg cattcgcaaa
     2881 atgcggtatg ttcagccgta aatcaggtgg tatgggcggt tatgccgagt atgctttcgg
     2941 taagtccggc aattttatgg cgaactacac ctacggtgtc tcgctgctga ttgctaacgt
     3001 cgcgattgct atttcggcgg ttggttacgg caccgaactg ctcggcgcga gtttgtcgcc
     3061 agtgcagatt ggtcttgcga ccatcggagt gctgtggatt tgtaccgtgg ctaactttgg
     3121 tggtgcgcgc attaccgggc aaatcagtag cattactgtg tggggggtca ttattccggt
     3181 ggtaggcctg tgcattatcg gctggttctg gtttagcccg acgctttacg ttgattcctg
     3241 gaatccgcat catgcaccgt tcttcagtgc agtaggttct tccatcgcta tgacgctgtg
     3301 ggcttttctc ggtctggagt ctgcgtgtgc gaatactgat gtagtggaaa acccggaacg
     3361 taatgtgcca atcgcggtac tcggcggtac gttaggtgcg gcggtgattt atatcgtctc
     3421 caccaacgtg attgcaggga ttgtgcctaa tatggagctg gcaaattcaa cggcaccatt
     3481 tggcctggcc ttcgcacaga tgttcacacc agaagtgggt aaagtcatta tggcgctgat
     3541 ggtgatgtcc tgctgcggtt cgctgcttgg ctggcagttc accattgccc aggtgtttaa
     3601 atcttcatct gatgaaggct acttccctaa aattttctcc cgtgtaacca aagtggatgc
     3661 accggtgcag ggaatgttga ccattgtgat tatccagagc gggttggcgc tgatgacgat
     3721 tagcccgtcg ctaaacagtc agttcaacgt gctggttaac ctggccgtgg tgaccaatat
     3781 cattccgtat attctgtcaa tggcggcatt agtcattatt cagaaggtcg ctaatgtgcc
     3841 cccctcaaaa gcgaaagttg caaactttgt tgcttttgtt ggcgcgatgt atagctttta
     3901 tgcgctgtac tcatccgggg aagaagccat gctgtacggt tccatcgtaa ccttccttgg
     3961 ttggacactg tatggtctgg tatcaccacg ctttgaactg aaaaataaac acggttaaaa
     4021 aagcaaagta aaaaagggcg atcttgcgac cgcccttttt ttattaaatg tgtttacgcg
     4081 tttttcagaa cttcgctaac aatctcaacc gcttctttct caatctgctt gcgatgttct
     4141 tcaccgagga agctttcgca gtagatctta tatgcgtctt ccgtgcctga cggacgcgcg
     4201 gcgaaccagc cgttgtcagt catcactttc agaccgccga tagaagcacc attgcccggt
     4261 gccgccgtca ggcgcgcggt gatcgggtcg cctgccaggg tgctggcgct caccatttcc
     4321 ggagacagct tagacagcgc cgctttttgt gcggaagtcg cagttgcctg caaacggttg
     4381 tagctcggcg caccaaagcg ttttgccagt tcgttgtagt gttcctgcgg gttcttaccg
     4441 gtgaccgcgg tgatttccgc cgccagcaga cacatgatga tgccgtcttt gtcggtggac
     4501 cacggcgtgc cgtcgaaacg caggaaggaa gcccctgcgc tctcttcgcc gccaaaaccg
     4561 aagctgccgt cgaacagacc atcaacaaac catttgaaac ctaccggtac ttctaccagc
     4621 ttacggtcca agtcattgac cacacggtcg atcatcgcag aagaaaccag cgttttaccg
     4681 acagcaacat ctttgcccca ctgcggacga tgctggaaca ggtagttgat cgccaccgcc
     4741 aggtagtggt tcggattcat caaacctgcc ggagtgacga taccgtgacg gtcataatcc
     4801 gggtcgttag caaacgccag atcgaactta tcacgcagtg ccagcaggcc cgccatcgca
     4861 cactcggagg agcagtccat acggatcgcg ccgtctttat caaggtgcat aaagcggaag
     4921 gtttgatcga cctgatcgtt aacgatggtc aggttgaggt tgtaatactc gccaatacgc
     4981 ttccagtatt cgataccgga accgcccagc ggatcaacgc ccagcgtcag gcccgctttc
     5041 tgaattgccg ccatatcaac gatatcggcc agaccttcca caaacggctg caccagatcc
     5101 tgctctttta catgaccgga tgccatcgct tcgtcgaggg agatacgctt cacgcctttc
     5161 aggccatcgg ccatcagtgc gttggccctg tcttccacca ctttggtgac gttggtatca
     5221 gccgggccac catttggcgg gttgtacttg ataccaccat cttccggcgg gttatgggac
     5281 ggtgtaatca cgataccgtc tgccagcggg ccaccttttt tattgtgaac caggatggca
     5341 ttggaaatgg caggtgtcgg agtgaagcca ttgttttcct gcacaatgac atcaacgccg
     5401 ttcgctgcca gcacttccag tacggaaatg aacgcaggct cggacagggc gtgagtatct
     5461 ttacccacat agcaagggcc agtgatgccg tttttcgcac gttcttcagc aattgcctga
     5521 gcgatcgcca gaatgtgcgg ctcgttaaag ctgtggcgcg ctgcactgcc acggtgaccg
     5581 gaagtaccga atttcaccgc gtgctccgca ttccctgctt ctggtttcag tacataatat
     5641 tgcgccgtca gttgggcgac gttaatcaaa tcactctgtt gtgcaggttg gcctgcacga
     5701 ttgtggattg ccattgcttt gtcctttgtc tgcaacgttt tagatagttc cgcaaacctt
     5761 ctcaatcaat tccgccggga attgcatcga ctgcatgatg tgttcgatca tgctgcattt
     5821 acggccggtg ttggtgttgg tgatcaccca atacggcgtg cctggcacat gtttcggctt
     5881 ggtctgatta ccatttttca gcagcgtttg ttcatctgcc gcaaagtaaa cgcgtgtacg
     5941 accgtgcaac gattccgttg cttcggcaaa cgcctgggcg tcaagagaat atagtgtaga
     6001 caacagcagc ataaagcgat tgaccgctcg cttttgctct gcgtattcgt ccgaaagcag
     6061 aagttcacgc attgcgcgaa ccttgtcttt aatcgttttg accggcttcg cttcgacgat
     6121 agcaggtgac gcaacgcgaa cctctttcgt caccggagca gcaggctgtg atgcggcgga
     6181 aaatttcaac atacgccgta aaatgtcgga tgcgctctcg ccgatatgct tagtgtggct
     6241 ggcaatatag ctgtagagtt catcatcaac ttcaatcgtt ttcatcttaa tccagtgcag
     6301 tgtcttagct gaatacaagt cgccaggagt atatgaataa atcccggcag cggatagcgt
     6361 caagcctggc ttgcagaaaa actcggaata aaccttattt gttgcagccc tgtggcacaa
     6421 tggtcaacat aataccctaa cccgacaacg cgaaaaaaag aactttgcca tgaaattgaa
     6481 tatccgcgcg caaactgcac aaaaccagca caataattct cccatcgttc ttgtccatgg
     6541 tctgtttggt agcctcgaca accttggcgt actggctcgc gatctggtaa acgatcacaa
     6601 tatcatccag gttgatatgc gtaaccacgg tctttcaccg agagagccgg taatgaatta
     6661 cccggcgatg gcgcaggatc ttgttgatac cctggatgca ctgcagatcg acaaagcaac
     6721 atttatcggt cactccatgg gcggtaaagc ggtaatggca cttactgcac tggcccccga
     6781 tcgcatcgat aaactggtgg cgatcgatat cgcaccggtc gactatcacg tacgccgtca
     6841 tgatgagatt ttcgcagcta tcaacgcggt cagtgaatcg gacgcacaaa ctcgccagca
     6901 agcagcagca ataatgcgcc agcatcttaa tgaagaaggg gtgattcagt ttctgttgaa
     6961 atcttttgtt gacggggagt ggcgctttaa cgtgccggta ttgtgggatc agtatccgca
     7021 tattgtgggt tgggagaaaa tcccggcatg ggatcaccct gccctgttta tccctggcgg
     7081 caattctccg tatgttagcg agcagtaccg tgatgattta ctggcacaat ttccacaggc
     7141 acgggcgcat gtgattgcag gcgcgggtca ctgggtccat gctgaaaaac cggatgcggt
     7201 attacgcgct atccgtcgct atctcaatga ttaacgcaac tgattaaaaa cccagcgtcc
     7261 gcgtcgcttt ccgcgcgtgg gcgttggcgt gactcatcgc gctgatgtat tatggcgcgc
     7321 tatccatccg ggcagaggcc tggctgattg tttcccccga agtcaccaag atcatggcca
     7381 aagaacaaac ggaccgtacg acattagatc tgttcgcgca cgagcgtcga ccgggacgac
     7441 cgaaaactaa tccgctttcg cgcgatgaac agctgcgtat taataaacgc aaccagctaa
     7501 aacgcgacaa agtacgtggc cttaagcgtg tcgaactgaa gctgaacgcg gaagctgtcg
     7561 aggcgctgaa cgagctggcg gagtcgcgca atatgagccg tagcgaactg atcgaagaga
     7621 tgttaatgca gcaactggcg gcgctgcgta gtcagggcat cgtttaaatt tccactttca
     7681 tgtagcacag tgtgcagtcc tgctcgtttg ctgattccgc aagactgcct gttctgctat
     7741 gattgccttt atccgtgggc aattttccac ccccatttca ataagtttca agaggttatt
     7801 tcactcatgg ctatcactgg catctttttc ggcagcgaca ccggtaatac cgaaaatatc
     7861 gcaaaaatga ttcaaaaaca gcttggtaaa gacgttgccg atgtccatga cattgcaaaa
     7921 agcagcaaag aagatctgga agcttatgac attctgctgc tgggcatccc aacctggtat
     7981 tatggcgaag cgcagtgtga ctgggatgac ttcttcccga ctctcgaaga gattgatttc
     8041 aatggcaaac tggttgcgct gtttggttgt ggtgaccagg aagattacgc cgaatatttc
     8101 tgcgatgcat tgggcaccat ccgcgacatc attgaaccgc gcggtgcaac gatcgttggt
     8161 cactggccaa ctgcgggcta tcatttcgaa gcatcaaaag gtctggctga tgacgaccac
     8221 tttgtcggtc tggctatcga cgaagaccgt cagccggaac tgaccgctga acgtgtagaa
     8281 aaatgggtta aacagatttc tgaagagttg catctcgacg aaattctcaa tgcctgatgt
     8341 gatgcggcgt agactcatgt ctacgccgta ttaatagata atgccaatca aaataattgc
     8401 tacaaatttg taacttttgc tgttgtacct gtacaatgtc ccggtgttca agtggccttg
     8461 ccgttgtaaa tgtaagctgt gccacgtttt tattaacaat atttgccagg gacttgtggt
     8521 tttcatttag gcgtggcaat tctataatga tacgcattat ctcaagagca aattctgtca
     8581 cttcttctaa tgaagtgaac cgcttagtaa caggacagat tccgcatgac tgataacaat
     8641 accgccctaa agaaagctgg cctgaaagta acgcttcctc gtttaaaaat cctggaagtt
     8701 cttcaggagc cggacaacca tcacgtcagt gcggaagatt tatacaaacg tctgatcgat
     8761 atgggtgaag aaattggtct ggctacggta tatcgcgtac tgaaccagtt tgacgacgct
     8821 ggtatcgtca cccgccacaa ttttgaaggc ggtaaatccg tatttgaact gacacagcaa
     8881 catcaccacg atcacctgat ctgcctcgac tgcggcaagg ttatcgaatt tagtgatgat
     8941 tccatcgaag cgcgtcagcg tgaaattgcc gcaaaacatg gcattcgcct gactaaccac
     9001 agtctctatc tttacggtca ctgtgccgaa ggcgattgcc gcgaagatga acatgcgcac
     9061 gaaggcaaat aagccagcct gaatgagaaa aagccaacct gcgggttggc ttttttatgc
     9121 aagggaaata agaaaagcga gagttacagc tctcacttat ttgttattac tgcgaatttc
     9181 ctgccaaact ttatcacaat cggctttcac cgcctgatca tttccggttt gcgttgcacg
     9241 cagacactgc tgataatcca gtacgcggac actttcctgg tcggcaaaat gctgatgttg
     9301 tttctctttc ttcagcacgt ttaacacgct ctggcaggct tcaatttttt ctggcgaacc
     9361 ttgtgcggtg ttgatacagg cgctgtatgc ctctttcaga cggctgtctt cctgtggcgc
     9421 ggttgattgc gcacaagcca ccagccccga tgccatcatg gcgatgagaa tcagtttttt
     9481 catagcaaat tcctcaaatg tgcggcaggc acaaaacctg ccgcgtcggg catcagaaga
     9541 tggtgaatgg tgcgattacc ataaatttca cgtcacgctc atcctggaag atgttgccgt
     9601 aaccaccgcc ccagcttggg atgtcggagt ggttgtcgta ttcggtgaaa tgcagtttga
     9661 acatcgtgcc tttggcgcga ccgtcctgaa tggtgtagac cgcatccagg ctgtatgcag
     9721 actcttcaat agtccggttt ttgtcgtagt acgcatccgg attgctctgc caggtcgcag
     9781 gtttagcatc ccatgcgtaa acgtaggaag cgccgatggc gaagcctgga agattccagt
     9841 ttttcaggtc atacatcgca ccgaagaaga ctgctttttc gccgttggcg ttgaagtcag
     9901 aacggttgtc ccaccagata tccaggcgac cgtttgagga agcgtaggtt ggagtcatac
     9961 gttgcaggaa gtatccctgc tgaccgtcag ccttaaccca ggtgccttcg aggcgcaaat
    10021 ccactacgtc agccgcccgg taaccaaagg tcaacgcctg cagccaggcg gtgccgtcat
    10081 aaaggtcgtt gacgctgcga tcgtcaactt tatcgcgggt accgtagaac tggtagctgg
    10141 tggttaacgg gctaccggcg atatcaaatt tgtagctggc tttggcaaaa tattgatcga
    10201 tatacccttc cgcctgacca aaagccgctt ccagtacgaa gttatttttg aagtcgtatt
    10261 tcgccccaag ggagtgcaga taatcaactt tggtggtttt atcgttctga taaaactcat
    10321 ccatttccag atgccacggc gctttgtatt cgttggtcca catgtaggag aaactcaacg
    10381 caccggcatc gccgtaatca aaattcgctc cggcttccgc accctgataa gtacccggca
    10441 taaagctcca gtgaggcgct aacagcgtct gaccggttgg ctgaatataa cctgccctcg
    10501 cccaaaccgg accgtattta aatttggccg ctgctttata caggcttata ccgcttttgt
    10561 cgccggacca gtcttcgtca taggctttat tactttttga aaacgcgatt tcgttcgggt
    10621 gagggctgtc gccgttttcc gccatttcaa tcgccgtaaa cgcggcaata tcaaggccga
    10681 acatatcagc agcataacca gactgaaaat cgaggttggc attccaggtg gagtgagaaa
    10741 ggttggtttt gtatttgtcg ccgtcggtaa catctttacg gtcgcgttca cgctgccagt
    10801 aatagatacc gccggttaag gttgaatcgt cgatgaatcc ttcggcgcga gcctccggca
    10861 ttgccataaa gcccgacatt gctgtaacac cggcgatagc cagcgccagc gtactacgtt
    10921 tgccactaaa cgtacgcatg ggttaatcct ctttgacgta taaattgctg caccaaaggt
    10981 gaacagcgaa aaaaaataaa aatttaaaaa gcagcgttga gaaaagtctc gctgactaca
    11041 ggaacagact atttttcgcg acgcgaatta tcaatctctt tctgtaacca gaatgtaaga
    11101 acatgagcaa ccgcacatat tttgatgatt tttgttacaa ggcttaagtt tgcggttgaa
    11161 aggcttgcag aagaataaaa gcgtgaattt ccagcaagat gaaagcggtt acattttatt
    11221 taattatgtt ttgttatttt aaatttattt tatggataaa ttgcgaagca ggattaattc
    11281 gcgtagaaaa gtagaaaaaa acgccacaga gcgcggcgtt ttggattgtt tgctgtttct
    11341 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag cacgcaaact caatatattg
    11401 cagagatcat gtaggcctga taagcgtagc gcatcaggca atttagcgtt tgaaactgtt
    11461 tgtctgttcc ttgccggatg cgacgtaaac gccccatccg gcatagcgaa acttaaaatt
    11521 actcgcctac tttcgcccag gtatcacgca gcccaacggt gcggttaaat accggttttt
    11581 ccgccgtaga atggcggcta tcgaggcaga agtaaccttc acgctcaaac tggaatgctt
    11641 tacccgcaac cgcatctttc agcgacggtt cagcaaagcc ctgtttgatc accagcgatt
    11701 ccgggttaat caccgacagg aaatcatccg cagcacctgg gttaggcacg ctgaacagac
    11761 ggtcatacaa acggatttca accggcagcg catgtgccgc gctcacccag tgaataacac
    11821 ctttgacttt acgaccatct gccggatctt tgcttaaggt atcggcgtca taagtacaga
    11881 agatggtggt gatattacct tcggcatctt tctcgacgcg ttctgcctta atcacataag
    11941 cattacgcag acgcacttct ttacccagca ccagacgttt gtactgcttg ttagcttctt
    12001 cgcggaaatc ggcgcgatca atccaaatct caccgctaaa cggcacctga cggctgccca
    12061 tttccggttt gttcggatgg ttcggcatgg taaccatttc gccttcgccc tgatagtttt
    12121 cgataaccag tttcaccgga tcgataaccg ccattgcgcg cggcgcattt tcgttgagat
    12181 cttcacggat gcaggattcc agcgacgcca tctcaatggt gttgtcctgc ttggtcacgc
    12241 cgatgcgttt gcagaactca cgaatagaag ccgcagtgta accacgacga cgcagaccgg
    12301 aaatggtcgg catacgcggg tcatcccagc cttcaacgtg cttgtcggtc accagcaggt
    12361 tcaacttacg cttggacatc acggtgtatt ccagattcag gcgcgagaac tcatactggc
    12421 gcgggtgaac aggaatcgtg atgttgtcca gtacccagtc gtacagacga cggttgtcct
    12481 ggaactcaag cgtacacaga gagtgcgtaa taccttccag ggcatcgctg atgcagtggg
    12541 tgaagtcgta catcgggtag atgcaccact tgttgccagt ctggtggtgt tcagcaaatt
    12601 taatacggta cagcaccgga tcgcgcatca cgataaacgg tgaagccatg tcgattttcg
    12661 cacgcaggca ggctttacct tcttcaaaac caccggcacg cattttttcg aacagcgcca
    12721 ggttctcttc aacgctgcgg tcgcggtacg ggctgttttt acccggttgc gtcagggtgc
    12781 cgcggtattc gcggatctgt tccggcgtca gttcatcaac gtacgccagg cctttattga
    12841 tcagttcgat cgcataggcg tggagctgat caaaataatc ggaggagtaa cggacgttac
    12901 cagaccagtg aaaacctaac cactctacgt cgtttttgat cgactcaaca tactcgatat
    12961 cttcttttac cgggttagtg tcgtcgaaac gcaggttgca ctggccttta tagtcctggg
    13021 cgatcccgaa gttcaggcag atagatttcg catggccaat atgcagatag ccattcggct
    13081 ccggcgggaa acgggtgtgt actgtggtgt gcttaccact ggccagatct tcatcgatga
    13141 tctgacggat aaagttagtc gggcgggctt ctgcctcact catcgtggat tcctcaaagc
    13201 gtaaacaacg tataacggcg tatgatctta taagcgggac aggctgacaa ctgttagttt
    13261 tttaaaattg ataatctaac aaggattatg gggatgattg atgaaaaaaa aagcggcaga
    13321 gaagatctcc accgcttttg ccagtcctgc gtgattgtcg gatgcaacgc taaagcgtct
    13381 tatccaacct ggactgagct tttgtaggcc tgataagacg cgtcaagcgt cgcatcaggc
    13441 atgaagcaga ttactttttg atttcataca gcggtgtttg acccgccaca acatggccct
    13501 gagctttaat gatcaagcca ctgaaatcgt cgatattgct gcaaaccacc ggactaatca
    13561 tcgagcgggc gttagcgttc aggtaatcca gatccatttc cagaatcggt tgccctgctc
    13621 ttacctgcgc gccctcttcc accagacgtt taaagccttt accttccagc gctacggtgt
    13681 cgatacccat gtggacgacg atctccgcgc ctttttcggt ttccagacag aacgcgtggt
    13741 tggtgttgaa gattttcacg attgtccctg cggctggtga tacgacgatt ttatctgtcg
    13801 gtttcaccgc cacaccgtca cccaccgctt tgctggcgaa tgcttcgtca ggaacctgat
    13861 ccagtgccac gacatcaccg gtaatcggcg ataccagctc cgcgatagat accgcgtttg
    13921 gtacagcctg cggttttgct acaggagcgg cagttgccgg agttgcttca gctgacgcag
    13981 ctgctaccgg gccacgggca acgactttct tcatcgcatc gccaatggat tctgctttcg
    14041 cgccaacaat cacctgaatg gtctgtttgt tcagttttac tacaccagaa gcacccagac
    14101 gtttacacat cgcatcgttg acgcgagctg agtcagccac ggtaaggcgc agacgggtaa
    14161 tacaggcgtc aatcgctttc aggttgtcag taccgccaac cgcagcaata tagttggttg
    14221 ccagttgagt cagaccttct tcagtattgc tgttagcctc ttcagttacg atctcgtctt
    14281 ctttatcttc acgacccggc gttttcagat tgaacatgcg gataaccaaa ctgaacacca
    14341 cgaagtagat agcgaagaag acaacaccca taaccagcag catccagacg ttctggctgg
    14401 cggccggcag gttatacatc aacgcgtagt cgatagcacc cgcagagaaa gagaagccag
    14461 catggatacc cagcagcgtt gccacaaaca ggctgatacc ggtcagcagt gcgtgcagga
    14521 ggtacagcag cggtgcaagg aacatgaaca ggaattccag cggctcagtc acaccggtca
    14581 ggaacgcagt aacagcaaca gaaagcagca tcccgccaac catcggacga cgctctttcg
    14641 gtgctgcgaa gtacatcgcc agcgccgcac ccggcagacc gaacatcatg atcgggaaga
    14701 agccggacat gaacatcccc gctgtgccgt caccagcgta gaaacggttg atgtcgccgt
    14761 ggaaaaccgt acccgccgcg ttggtgaatt caccaatctg gaaccaggcg atggtgttca
    14821 gcacctgatg cagaccggtt gggatcagca gacggttgat gaaaccaaag ataccggaac
    14881 ccagcgcgcc cgcagaaacg atccactcgc cgcctgcatg gatagcgtgc tgtaccggcg
    14941 gccagacgta accaaaaatg gccgccagca ccagacagaa gaagccggtg gcgatcggca
    15001 caaagcgttt gccgccgaag aagctcagga agtccggcag tttaatatcg gaccaacggt
    15061 tataggctgc gccaccaacc agaccggtaa tgatacccgc cagtacaccc atgttaattt
    15121 ctgggttgat ggtcaccatc gctttggtta acacaaagta acctaccgca cccgccagcg
    15181 ccgccgcacc tgcgctgtct ttcgaccagc tggatgccac accgatggcg aagattaatg
    15241 cgaggttatc aaaaatcgca ccgcccgcct gggcaataaa cgcaacgtta agtaaatctg
    15301 gctgaccgaa tcgcagcaac agtgccgcca ccggcagcac cgcgataggg agctgtaacg
    15361 ccctaccgag tcgctggaaa aaacctaaaa tattcatctt attcccccta cgagaaccct
    15421 atttggctcg tttaaagccg tatttttatt ttgctgcaaa ttgtactgcc gatgttctgt
    15481 aatcagattg ttagatcatc tgctacagag tgtgtgaaaa tttaattcgc atcgcaaatt
    15541 aaatgcgtgt cttttgtgag ttttgtcacc aaatatcgtt atcatcactc ccttttactg
    15601 gctaaaccag aaaacttatt ttatcattca aaaaatcagg tcggattgac gcctgtctgc
    15661 gcaaatccag gttacgctta aagatgccta atccgccaac ggcttacatt ttacttattg
    15721 aggtgaataa tgagactgat ccccctgact accgctgaac aggtcggcaa atgggctgct
    15781 cgccatatcg tcaatcgtat caatgcgttc aaaccgactg ccgatcgtcc gtttgtactg
    15841 ggcctgccga ctggcggcac gccgatgacc acctataaag cgttagtcga aatgcataaa
    15901 gcaggccagg tcagctttaa gcacgttgtc accttcaaca tggacgaata tgtcggtctg
    15961 ccgaaagagc atccggaaag ctactacagc tttatgcacc gtaatttctt cgatcacgtt
    16021 gatattccag cagaaaacat caaccttctc aacggcaatg ccccggatat cgacgccgag
    16081 tgccgccagt atgaagaaaa aatccgttct tacggaaaaa ttcatctgtt tatgggcggt
    16141 gtaggtaacg acggtcatat tgcatttaac gaaccggcgt cttctctggc ttctcgtact
    16201 cgtatcaaaa ccctgactca tgacactcgc gtcgcgaact ctcgtttctt tgataacgat
    16261 gtcaatcagg tgccaaaata tgccctgact gtcggtgttg gtacattgct ggatgccgaa
    16321 gaagtgatga ttctggtgct gggtagccag aaagcactgg cactgcaggc tgcagttgaa
    16381 ggttgcgtga accacatgtg gaccatcagc tgtctgcaac tgcatccaaa agcgatcatg
    16441 gtgtgcgatg aaccttccac catggagctg aaagttaaga ctttaagata tttcaatgaa
    16501 ttagaagcag aaaatatcaa aggtctgtaa ttgttatccc tgccctggct ccttgctcag
    16561 ggcaatattt tttaaaatcg ggggtcagaa tgtatgcatt aacccagggc cggatcttta
    16621 ccggccacga atttcttgat gaccacgcgg ttgttatcgc tgatggcctg attaaaagcg
    16681 tctgtccggt agcggaactg ccgccagaga tcgaacaacg ttcactgaac ggggccattc
    16741 tctcccccgg ttttatcgat gtgcagttaa acggctgcgg cggcgtacag tttaacgaca
    16801 ccgctgaagc ggtcagcgtg gaaacgctgg aaatcatgca gaaagccaat gagaaatcag
    16861 gctgtactaa ctatctgccg acgcttatca ccaccagcga tgagctgatg aaacagggcg
    16921 tgcgcgttat gcgcgagtac ctggcaaaac atcccaatca ggcgttaggt ctgcatctgg
    16981 aaggtccgtg gctgaatctg gtaaaaaaag gcacccataa tccgaatttt gtgcgtaagc
    17041 ctgatgccgc gctggtcgat ttcctgtgtg aaaacgccga cgtcattacc aaagtgaccc
    17101 tggcaccgga aatggttcct gcggaagtca tcagcaaact ggcaaatgcc gggattgtgg
    17161 tttctgccgg tcactccaac gcgacgttga aagaagcgaa agccggtttc cgcgcgggga
    17221 ttacctttgc cacccatctg tacaatgcga tgccgtatat taccggtcgt gaaccgggcc
    17281 tggcgggcgc gatcctcgac gaagctgaca tttattgcgg tattatcgct gatggcctgc
    17341 atgttgatta cgccaacatt cgtaacgcta aacgccttaa aggcgacaaa ctgtgtctgg
    17401 ttaccgacgc caccgcgcca gcaggtgcca acattgaaca gttcattttt gcgggtaaaa
    17461 caatatacta ccgtaacgga ctttgtgtgg atgagaacgg tacgttaagc ggttcatcct
    17521 taaccatgat tgaaggcgtg cgtaatctgg tcgaacattg cggtatcgca ctggatgaag
    17581 tgctgcgtat ggcgacgctc tatccggcgc gtgcgattgg cgttgagaaa cgtctcggca
    17641 cactcgccgc aggtaaagta gccaacctga ccgcattcac acctgatttt aaaatcacca
    17701 agaccatcgt taacggtaac gaggtcgtaa ctcaataaga gaaagtatga caccaggcgg
    17761 acaagctcag ataggtaatg ttgatctcgt aaaacagctt aacagcgcgg cggtttatcg
    17821 cctgattgac cagtacgggc caatctcgcg gattcagatt gccgagcaaa gccagcttgc
    17881 ccccgccagc gtaaccaaaa ttacgcgtca gcttatcgaa cgcgggctga tcaaagaagt
    17941 tgatcagcag gcctccaccg ggggccgccg cgctatctcc atcgtcaccg aaacccgcaa
    18001 tttccacgca atcggcgtac ggcttggtcg tcatgacgcc accatcactc tgtttgatct
    18061 cagcagcaaa gtgctggcag aagaacatta cccgctgccg gaacgtaccc agcagacgct
    18121 ggaacatgcc ctgctgaatg ccattgctca gtttattgat agctaccaac gcaagctgcg
    18181 cgagctgatc gccatttcgg tgatcctgcc agggcttgtt gacccggaca gcggcaaaat
    18241 tcattacatg ccgcatattc aggtagaaaa ctgggggctg gtagaagcac tggaagaacg
    18301 ttttaaagtg acctgtttcg ttggtcacga tatccgtagt ctggcgctgg cggagcacta
    18361 cttcggtgca agtcaggatt gcgaagactc cattctggtg cgtgtccatc gcggaaccgg
    18421 ggccgggatt atctctaacg ggcgcatttt tattggccgc aacggcaacg tcggtgaaat
    18481 tggccatatt caggtcgaac cgctgggtga acgctgccac tgcggcaact ttggctgcct
    18541 ggaaactatc gccgccaacg ctgccattga acaacgggtg ttgaatctgt taaagcaggg
    18601 ctaccagagc cgtgtgccac tggacgactg caccatcaaa actatctgca aagccgcgaa
    18661 caaaggcgat agcctggcct cggaagtgat tgagtatgtc ggtcgtcatc tgggcaaaac
    18721 catcgccatt gctatcaact tatttaatcc gcaaaaaatt gttatcgctg gtgaaatcac
    18781 cgaagccgat aaagtgctgc tccctgctat tgaaagctgc attaataccc aggcgctgaa
    18841 ggcgtttcgc actaatctgc cggtggtacg ttctgagctg gatcaccgct cggcaatcgg
    18901 cgcttttgcg ctggtaaaac gcgccatgct caacggtatt ttgctccagc atttgctgga
    18961 aaattaatgt gcttttatag tggcgcttat tgttgtcaat attctgggta gtccatgacc
    19021 attaaaaatg taatttgcga tatcgacggc gtgctgatgc acgataacgt cgccgtaccg
    19081 ggtgcagcgg aatttttgca cgggattatg gataaaggcc tgccgctggt gttgctgacc
    19141 aactatcctt cgcagaccgg gcaggatctg gcgaaccgct ttgccactgc cggtgtcgat
    19201 gtacctgaca gcgtgtttta tacctctgcg atggcgaccg ccgatttcct gcgtcgccag
    19261 gaaggcaaga aagcgtatgt ggtgggcgaa ggcgcactga ttcatgaact gtacaaagcc
    19321 ggtttcacta ttaccgatgt gaaccctgat tttgtgattg ttggcgaaac gcgttcctac
    19381 aactgggaca tgatgcataa agcggcctat ttcgtcgcta acggtgcacg ttttatcgcc
    19441 accaacccgg acacccacgg gcgcggtttt tatcccgcct gtggcgcgtt gtgtgcaggg
    19501 atcgaaaaaa tctccgggcg taaaccgttc tatgttggta agccgagtcc gtggatcatc
    19561 cgcgcggcat taaacaaaat gcaggcgcat tcggaagaaa cggtgattgt cggcgataac
    19621 ctgcgtaccg atattctggc cggcttccag gcaggtctgg agacgattct ggtgctttct
    19681 ggtgtttcgt cgctcgacga tatcgacagt atgcctttcc gccccagctg gatttacccg
    19741 tcagtcgctg aaatcgacgt tatctgaata gcaaccacgt ctcaggacgt ggttggctct
    19801 gatgacaaac gccaaactgc ctgatgcgct acgcttatca ggcctacgca gctcctgcaa
    19861 tatattgaat tttcatgctt ttgtaggccg gataaggcgt tcacgccgca tccggcatga
    19921 acaaatcgca ctttgtcagc aatttggccc ccgtaaaata taaacattca ataaaacccg
    19981 ctaaaaatta tcgattcatc aataaatata tcctcacacc agaccttttt tcaccaatcg
    20041 acaatcacca ttacgttttt tattttttca ccgccaaatc gcttgcgcac agtgctgctt
    20101 tgcggcattt ttttaaacaa gcaaacacaa caagcaacaa atacaaggtt aacggagaag
    20161 gttatgtgtt caatttttgg cgtattcgat atcaaaacag acgcagttga gctgcgtaag
    20221 aaagccctcg agctgtcacg cctgatgcgt catcgtggcc cggactggtc cggtatttat
    20281 gccagcgata acgccattct cgcccacgaa cgtctgtcaa ttgttgacgt taacgcgggg
    20341 gcgcaacctc tctacaacca acaaaaaacc cacgtactgg cggtaaacgg tgaaatctac
    20401 aaccaccagg cattgcgcgc cgaatatggc gatcgttacc agttccagac cgggtctgac
    20461 tgtgaagtga tcctcgcgct gtatcaggaa aaagggccgg aatttctcga cgacttgcag
    20521 ggcatgtttg cctttgcact gtacgacagc gaaaaagatg cctacctgat tggtcgcgac
    20581 catctgggga tcatcccact gtatatgggg tatgacgaac acggtcagct gtatgtggcc
    20641 tcagaaatga aagcgctggt gccagtttgc cgcacgatta aagagttccc ggcggggagc
    20701 tatttgtgga gccaggacgg cgaaatccgt tcttactatc atcgcgactg gttcgactac
    20761 gatgcggtga aagataacgt gaccgacaaa aacgagctgc gtcaggcact ggaagattca
    20821 gttaaaagcc atctgatgtc tgatgtgcct tacggtgtgc tgctttctgg tggtctggat
    20881 tcctcaatta tttccgctat caccaagaaa tacgcagccc gtcgcgtgga agatcaggaa
    20941 cgctctgaag cctggtggcc gcagttacac tcctttgctg taggtctgcc gggttcaccg
    21001 gatctgaaag cagcccagga agtggcaaac catctgggca cggtgcatca cgaaattcac
    21061 ttcactgtac aggaaggtct ggatgccatc cgcgacgtga tttaccacat cgaaacttat
    21121 gatgtgacca ctattcgcgc ttcaacaccg atgtatttaa tgtcgcgtaa gatcaaggcg
    21181 atgggcatta aaatggtgct gtccggtgaa ggttctgatg aagtgtttgg cggttatctt
    21241 tacttccaca aagcgccgaa cgccaaagaa ctgcatgaag agacggtgcg taaactgctg
    21301 gccctgcata tgtatgactg cgcccgtgcc aacaaagcga tgtccgcctg gggcgtggaa
    21361 gcgcgcgttc cgttcctcga taagaaattc cttgatgtgg cgatgcgtat taacccacag
    21421 gataaaatgt gcggtaacgg caaaatggaa aaacacatcc tgcgtgaatg ttttgaagcg
    21481 tatctgcctg caagcgtggc ctggcggcag aaagagcagt tctccgatgg agtcggttac
    21541 agttggatcg acaccctgaa agaagtggcg gcgcagcagg ttaccgatca gcaactggaa
    21601 actgcccgct tccgcttccc atacaacacg ccaacctcta aagaagcgta tctgtaccgg
    21661 gagatctttg aagaactgtt cccgcttccg agcgccgctg agtgcgtgcc tggcggtcct
    21721 tccgtcgcgt gttcttccgc taaagcgatc gaatgggatg aagcgttcaa gaaaatggac
    21781 gatccatctg gtcgtgcggt tggtgttcac cagtcggcat ataagtaaga aatgcgaaac
    21841 agccccggtg aaatccgggg ctgtttcagt tattgaggtg cttgttgatt ctgatatgca
    21901 gtaaggaaat gttccgggtt tgccacatca ctgtttgaca actcatcact gtaggccagt
    21961 atttcataga ttaatttccc accactattg ttgaattccg aatttcatcc tgtatgacaa
    22021 aagaatggct gtagggtggg aaaaaacaca aagtaaacaa taattgacga atatagcgcc
    22081 acgctgttcg caacctaacc aaacagtcac tttcgagcaa ttttccttga aaaagaggtt
    22141 gacgctgcaa ggctctatac gcataatgcg ccccgcaacg ccgataaggt atcgcgaaaa
    22201 aaagatggct acgtagctca gttggttaga gcacatcact cataatgatg gggtcacagg
    22261 ttcgaatccc gtcgtagcca ccatcttttt tgcgggagtg gcgaaattgg tagacgcacc
    22321 agatttaggt tctggcgccg caaggtgtgc gagttcaagt ctcgcctccc gcaccattca
    22381 ccagaaagcg ttgtacggat ggggtatcgc caagcggtaa ggcaccggtt tttgataccg
    22441 gcattccctg gttcgaatcc aggtacccca gccatcgaag aaacaatctg gctacgtagc
    22501 tcagttggtt agagcacatc actcataatg atggggtcac aggttcgaat cccgtcgtag
    22561 ccaccaaatt ctgaatgtgt cgaatacgtt cggcaaattc aaaaaccaat ttgttggggt
    22621 atcgccaagc ggtaaggcac cggattctga ttccggcatt ccgaggttcg aatcctcgta
    22681 ccccagccac attaaaaaag ctcgcttcgg cgagcttttt gcttttctgc gttcattcaa
    22741 tgtcgaatgc gatgttgaca cgtcttatcc ttcaatgtcg gatgcgacgc tgccgcgtct
    22801 tatccgacct acggttggca cgcatccggc aatgttgtaa ggctacaacc ctaacgcata
    22861 tttcagcgcc tgacgtttca acacgccagc acgctccgcc gccattaacc cgagattacg
    22921 cataaaacgc agtggtggca gattattgct gaatccggca taaaacagat ccataccgct
    22981 ttgcataatg aagttatccg ccatgcgccg catctggtaa cgcttgagga ttggataact
    23041 ggcccacgct tcgccgtagc tgcgggcgtt gaccagaaca tcaatcaggg catcgacatc
    23101 acgataacca agattcaccc cctgccccgc cagcggatgg atggtatgcg cggcatcgcc
    23161 caccagcgca agccctggct gcacatactg caacgcatgg cgacgcgtca gcggaaacgc
    23221 accagcggca agcggtgtaa cgtaacccag acgcgacggg aaatgcttcg cgatttccgc
    23281 ctgtaactgc gccatgttca tattctgcaa ctggcgaata cgcgccggag agtcatacca
    23341 caccagcgat gcccagttat caaacaacgg cagaaacgca cgcggtccgt ccggagtaaa
    23401 ttgctgccag gtgctgtcgc cgggatcgtt ctcacactgt acgctaatca acatacacga
    23461 ctgcgcatac tgccaggcat gaacgccaat acccgccatc tgccgcacct gcgaatttgc
    23521 gccgtcggca ccaatcacca gcttcgcgcg gatcgtttct ccacccttca gttccagctc
    23581 ctgaagatca tcatggcgat gcagcgcaat cagtgagcct ggcacacgta acgttacttt
    23641 cggatgcgct tccagcgcct gccacagcgc ctgttgcagg acggtgtttt ccaccatata
    23701 gccaagcaac ggtagcttaa gttcagcggc gtcaaacacc acatgcgccg tttcccactc
    23761 ccacgtttcc agtctgcggt aaggatggca acgcatagcc tgtactgcat cccagacccc
    23821 taacccttta agcaatgata ccgaagccgc gctgatcgcc gagatccgca catccggttg
    23881 gctatcagcg acaaacggtg caggttctgc gtgctcgatc accgttaccg taaatccgtg
    23941 ctgtgccagc cccagcgcca gtgcgccgcc gaccattcct acgccgacaa tggcaatttc
    24001 cgttggttga tttgtcatgg tcaatcatcc tgttagcaat atgctttaag tttacaggat
    24061 ttttaccccc aagggctgat aacgctcata ctggtcacaa cggcagcaaa gcattacact
    24121 atgcgccctg cattcctggc tactatttcg caagagcaag tcgatgacca aaaaactcca
    24181 tattaaaacc tggggctgtc agatgaacga gtacgattca tcgaagatgg ccgatctgct
    24241 ggatgccacc cacggctatc aactgaccga cgtggcggaa gaagcggatg tgctgctgct
    24301 gaacacctgc tcaatccgcg agaaggctca ggaaaaagtc ttccatcagt tggggcgctg
    24361 gaaactgtta aaagagaaga atccagacct gattatcggc gtcggtggct gcgtggcatc
    24421 gcaagaaggc gagcacattc gccagcgcgc ccactatgtc gatattattt ttgggccgca
    24481 aacgctgcat cgtctgccgg agatgatcaa ctccgtgcgc ggcgaccgca gcccggttgt
    24541 agatatcagc ttcccggaaa tcgagaagtt tgaccgtctg ccggaaccgc gcgccgaagg
    24601 gccgaccgcg tttgtctcca tcatggaagg ctgcaataaa tattgcacct actgcgtggt
    24661 gccttacacc cgtggtgaag aggtaagccg tccgtccgac gatattctgt ttgaaattgc
    24721 ccagcttgcg gctcagggcg tgcgtgaagt caacctgctc ggtcagaacg tgaacgcctg
    24781 gcgtggtgaa aactacgacg gcaccactgg atcgtttgcc gatctgctgc gtctggttgc
    24841 ggcaatcgac gggatcgatc gtattcgctt taccaccagc catccgatcg aattcaccga
    24901 cgatatcatc gaagtgtatc gcgacacgcc ggagctggtg agcttcctgc atctgccggt
    24961 acagagcggt tccgatcgca ttctgaacct gatgggccgt acccatacgg cgctggagta
    25021 caaagcgatc atccgtaaac tgcgtgcggc gcgtccggat attcagatca gttctgactt
    25081 catcgttggc ttccctggag aaaccaccga agacttcgag aaaacgatga agctgattgc
    25141 cgacgtcaat ttcgacatga gctacagctt tatcttctct gcacgtccag gaacgcctgc
    25201 cgctgatatg gttgatgatg ttccggaaga agagaagaag cagcgtctgt atattctgca
    25261 agaacgtatc aatcagcaag cgatggcatg gagccgtcgg atgctcggca ccacccagcg
    25321 tattctggta gaaggtacat cgcgtaagag catcatggag ctttccgggc gtaccgaaaa
    25381 taaccgtgtg gtcaacttcg agggcacacc ggatatgatc ggtaaattcg tcgatgtaga
    25441 aattaccgac gtctacccga actctctgcg cggtaaagtg gtgcgtactg aagatgaaat
    25501 gggtctgcgc gtggcagaaa cacccgaatc ggtgattgcc cgtactcgca aagaaaatga
    25561 ccttggcgtg ggttattatc agccgtaatt ctcaggcccg ccgttccggt gggccttgtt
    25621 ttttctctct ttatccccat ctttatcgca atgcttgcgc ctttttcctg tggcgtgaat
    25681 aatttcctta taggccggtg aatgttcagc ttccggcggc atacagataa gaggaacggt
    25741 ttgaacatag acactcgcga aatcaccctg gagccagcag acaatgcgcg tctgttgagc
    25801 ctgtgcggcc cgtttgatga caacatcaag cagctcgaac gtcgtctcgg catcgagatc
    25861 aatcgccgcg ataaccactt taaactgacc ggccgtccga tttgcgtcac cgctgcggca
    25921 gacattctgc gtagcctgta tgtcgatact gccccgatgc gcggtcagat tcaggatatc
    25981 gaaccggaac agatccacct tgcgattaaa gaagcgcggg tactggagca aagcgcggag
    26041 agcgtgccgg agtacggcaa agcggtcaat atcaaaacta aacgcggcgt aattaagccg
    26101 cgcacgccaa accaggcgca gtacatcgcc aatattctcg accatgacat taccttcggc
    26161 gttggcccgg cgggtacggg taaaacctac ctggcagtgg ctgcggcagt tgatgccctg
    26221 gagcgtcagg aaattcgccg tattctgctg actcgtccgg cagtagaagc cggtgagaaa
    26281 ctgggcttcc tgcctggcga tttaagccag aaagtagacc cgtatctgcg cccactgtac
    26341 gacgcgctgt ttgaaatgct gggctttgag aaagtcgaga aactgattga gcgcaacgtt
    26401 attgaagtcg cgcctctggc ctatatgcgt ggtcgtacgc tgaatgacgc ctttatcatt
    26461 ctcgatgaga gccagaacac caccatcgaa cagatgaaga tgttcctgac ccgtatcggt
    26521 tttaactcaa aagcggttat caccggcgac gtcacacaga tcgacctgcc gcgtaatact
    26581 aaatcaggtt tgcgtcatgc catcgaagtg ctggccgatg tcgaagagat cagctttaac
    26641 ttcttccaca gcgaagacgt ggttcgtcac ccggtggtgg cgcgtatcgt taacgcttat
    26701 gaagcctggg aagaagcaga acaaaaacga aaagcggcgc tggcggcaga acgcaagcgc
    26761 gaagaacagg aacaaaaatg agtcaggtga tcctcgattt acaactggca tgtgaagata
    26821 attccgggtt accggaagag agccagtttc agacatggct gaatgcggtg atcccgcagt
    26881 ttcagaaaga atcggaagtg actattcgcg tggtcgatac cgccgaaagc cacagtctga
    26941 atctgaccta tcgcggtaag gataagccga ccaacgtgct ctccttcccg tttgaagtgc
    27001 cgcctggcat ggagatgtcg ctactgggcg atctggttat ctgccgtcag gtggttgaga
    27061 aggaagctca ggagcaaggc aaaccactgg aggcgcactg ggcgcatatg gtggtgcacg
    27121 gcagtctgca tttgttaggt tacgatcaca tcgaagatga cgaagcagaa gaaatggaag
    27181 ccctcgaaac agagattatg cttgctctgg gctatgagga tccgtacatt gccgagaaag
    27241 aataatttgc caggcatttg actggcagac gtccccgccg ttggtgatta acgacggcgt
    27301 tttgattaac taacatgaga acccatacga cgccatgagc gacgacaatt cacacagtag
    27361 tgacacgata agcaacaaga agggattttt ctccctgtta ctcagccaac ttttccacgg
    27421 tgaaccgaaa aaccgtgacg aactgctggc gctgatccgt gattccgggc agaacgacct
    27481 tatcgacgaa gatacgcgcg atatgctcga aggggtgatg gacatcgcag accaacgcgt
    27541 tcgcgacatc atgatccccc gctcccagat gattaccctg aaacgcaacc agacgctgga
    27601 cgaatgcctt gatgtcatca tcgagtccgc ccactcacgt ttcccggtga ttagcgaaga
    27661 caaagatcac attgaaggga ttctgatggc gaaagacttg ctgccgttta tgcgcagcga
    27721 tgctgaagcc ttcagcatgg acaaagtgtt acgtcaggcg gttgtcgttc ctgaaagtaa
    27781 gcgcgtagac cggatgctga aagagtttcg ctctcagcgt taccacatgg cgatcgtcat
    27841 tgacgaattc ggtggggttt ccggtctggt gaccattgaa gacatcctgg aactaattgt
    27901 tggtgagatt gaagacgagt atgacgaaga agatgatatc gacttccgtc agctgagtcg
    27961 tcatacctgg accgtgcgcg cactggcttc cattgaagac ttcaacgaag cgttcggcac
    28021 ccactttagc gatgaagagg ttgacactat cggtggtctg gtgatgcagg catttgggca
    28081 tcttccggcg cgtggcgaaa ctatcgacat cgacggttac cagttcaaag tggcgatggc
    28141 cgacagtcgg cgtattattc aggttcatgt caaaatcccg gatgactcac cccagccaaa
    28201 gctggatgaa taaaaccgaa actggataga taactacatg gcttttgcct cattaattga
    28261 acgccagcgc attcgcctgc tgctggcgtt attattcggt gcctgcggaa cgctggcctt
    28321 ctctccttac gacgtctggc ctgcggcgat tatttcgctg atggggcttc aggcgttgac
    28381 ctttaaccgt cgcccactcc agtctgccgc tattggcttt tgctggggat ttggcctctt
    28441 tggcagcggt attaactggg tctatgtcag catcgcgacc tttggcggga tgcctggccc
    28501 ggttaacatc ttcctggtgg tgctgctggc ggcgtatttg tcgctgtata ccggactgtt
    28561 tgctggcgtg ctgtcgcgtc tgtggccgaa aaccacctgg ctgcgcgtgg cgattgccgc
    28621 cccttctctc tggcaagtga ccgagtttct gcgcggttgg gtactgacag gcttcccgtg
    28681 gttacagttc ggctatagcc agattgatgg cccgttaaaa gggctggcac cgataatggg
    28741 cgtggaagcc attaacttcc tgctgatgat ggttagtggc ctgctggcac tggcgttggt
    28801 caaacgcaac tggcgtccgc tggtggtggc cgtcgtgctg tttgccctac ccttcccgct
    28861 gcgttacatc caatggttta ccccacaacc ggagaaaacc attcaggttt cgatggttca
    28921 gggcgatatt ccgcaatcac tgaaatggga tggagatcag cttcttaata cgctgaagat
    28981 ttactacaac gcaacggctc cgctgatggg caaatcatcg ttgattatct ggccggagtc
    29041 ggcgataacc gatctggaaa ttaatcagca accattcctc aaagcactgg acggtgagtt
    29101 gcgcgataaa ggtagctcgc tcgtaaccgg gattgtcgac gcgcgtctca ataagcagaa
    29161 ccgctacgat acctacaaca ccatcatcac actgggtaaa ggtgcaccgt acagctacga
    29221 atcagccgat cgctataaca aaaaccatct ggtgccgttt ggcgagtttg tcccgctgga
    29281 gtcgattctg cgtccgttag caccgttctt tgatctgccg atgtcgtcgt tcagccgtgg
    29341 gccatatatc cagccgccgc tgtcggtaaa tggtattgag cttactgcgg ccatttgcta
    29401 cgagatcatt cttggcgagc aagtgcgcga taacttccgc ccggataccg actatctgct
    29461 gactatctcc aacgatgcgt ggtttggtaa atctattggt ccatggcaac acttccagat
    29521 ggcgcgaatg cgtgcgctgg agctggcgcg cccactgttg cgcagcacca acaacggcat
    29581 tacggcggtg attggcccgc agggtgagat tcaggcgatg atcccgcagt tcacccgcga
    29641 ggtgttgacc actaacgtga cgccgaccac cggactcaca ccgtatgcac gaaccggcaa
    29701 ctggccgctg tgggtgctga cggcactgtt tggttttgcc gctgtgctga tgagtctgcg
    29761 agcacgtaaa cgttgatgtg cctgatgtat cctgcatgag caaaacgtac tttctcattg
    29821 agctttgggg catggttatc atgccccttt ttcttacggt tcagggatac aagccacatt
    29881 gccatattgg ggatgacgaa taattgcctg cggatcaagc gagcgcgtcc tgtccagttg
    29941 gatcaacccg ttcttcgcca taacaaatat ttttctacca tccgctgaaa aacgaaggtg
    30001 attaacgtag ctggcaggat aaacgccaat aatctgcccg gcattaatat tgagcaggat
    30061 gacataacct tgcaggccat atgacaccaa cgctaaatac tgaccgtccg ggctaatacc
    30121 ctcaacggaa taaacgctcc tgctacacca ttgcgtcata atgctggctt gttgatcccc
    30181 tacgcttata cgtgaaatcc cctggctgtc ggcaaaaatt acatccccgt tttcaggtcg
    30241 aaaacgcatc gccgatatcg ttcctcgcgc atcactagcc ggatagcttt ctttctgatg
    30301 cgtgatggta ttgatgcgta caatgtcacc tctgaccata ccgggtatca acaatatttt
    30361 ttcatccggg gaaagagcca taacccaggc accaggttta ttactcgtcc ccgtcccgtc
    30421 gtatttctcc agcgggatat ccgccacatg ctgataagtc accagatcaa tcgctgacac
    30481 actattactg gtgcgattca tcacccaggc ttttgtgcca tcacgcgaga ggataaggct
    30541 gtcaggatgt gcaccggtgg taatcgactg aataatctcc cctgtcgcca cgttaatgac
    30601 cgataacgca ttcgcttcaa atgaagtcgc ataaatcaac ttttcatccg ggcttaatgc
    30661 cagcgatctg acgccctgat ctttacagcc accacaatgt ttgactgact tttctttcgc
    30721 tatgggcgct gtaaaggtat gcaggatttt cttttgtccg aggtcgaaaa caacaatgct
    30781 gttcgtgttt tcaattgcgg catacaacat attaccatcc cgactgatcg ccatatcatg
    30841 gatatcggca atctcagcga gcaaatcacc actggcctgt tcacgactta ccggagcgga
    30901 taatgtttcc ctgagggcgt tgcgttcctc cgctaactgc tgcttttttt gccgtcgctc
    30961 ttgttcaatc tcctgtggat aaaacgcttc acgcaggctt tgcctgactt gtaaaactga
    31021 ctcatccatc tgctgctgcg caagccagat agccccggtc agaagaagac tgcccgcaac
    31081 aataatcccg ccttccgtaa cgagtcggtt ttgcccagat aaacgcgcgc tcagccaacc
    31141 caacgcgcca cccgcaatca ggccgccaat gtgacaagca ttatctattc ctgtttgccg
    31201 ggcaccatac aacagtgtca gcgccaccat acccaacaga gaaaatactc gcctctggtt
    31261 tttatggtat gtgcccgcac ctgaagcaaa ctgtgttgct atcgctgcac cagctattcc
    31321 cagaatcgcc ccggatgccc caacgctgat gactatctgc tcactttcgc gtaacgcata
    31381 acaggcgctg atgagcccac caaagacgcc ggagaataac caggtaatca ggagtcgcca
    31441 tttccccaga atctgttccg ccacgcctcc cacgaccagg aatgcaaaca tattcatcag
    31501 cagatgagaa acactactgt gcagaaagat actgctcaat aagcgccagg gttcacccga
    31561 caaagtaaga ggcgcaacgt ttgcgccgag atggataagt gcggattgat cgacctcttc
    31621 ataactcatc cattgtgcga gataacaaaa tatcccaata cagagtgcaa aaaggataag
    31681 ggttattgcg ggtaattgaa catttaacgg cttaaccgaa gatgcagaca taaggtgttt
    31741 cttccttgaa aagacaggta aaaagcattt ttcccgtgtg cagttatcat cccggcaaac
    31801 gtcccatgcc gaaaagataa ttctgtccga aatatgactt tttcagccta aaattacaat
    31861 cgtaattaat tcgcccctaa gtattttttg gcatgtaagt tgctttattt actgtgtaag
    31921 cgatgaaatc agccgcgacg caaccaggtt gcaccaccac aaatcagcgg tagcaccaaa
    31981 aaggtgcggt ttaagaattt tgcttcggaa tggtgcggcg catctcgcaa aaacaaacaa
    32041 taaaacagca atatctttac atttagccaa actaaatgtt acctaacagg cacaacactg
    32101 cacaataaag ttgcagacga taacaacaca aacactcaca acgggtatcc atgcgttctt
    32161 aacgcagaag ataaaggagt tggatatgca attacgtaaa cctgccacag caatcctcgc
    32221 cctggcgctt tccgcaggac tggcacaggc agatgacgcc gccccggcag caggcagtac
    32281 gctggacaaa atcgccaaaa acggtgtgat tgtcgtcggt caccgtgaat cttcagtacc
    32341 tttctcttat tacgacaatc agcaaaaagt ggtgggttac tcgcaggatt actccaacgc
    32401 cattgttgaa gcagtgaaaa agaaactcaa caaaccggac ttgcaggtga aactgattcc
    32461 gattacctca caaaaccgta ttccactgct gcaaaacggc actttcgatt ttgaatgtgg
    32521 ttctaccact aacaacgtcg aacgccaaaa acaggcggct ttctctgaca ctattttcgt
    32581 ggtcggtacg cgcctgctga ccaaaaaggg tggcgatatc aaagattttg ccgacctgaa
    32641 agacaaagcc gtagtcgtca cttccggcac tacctctgaa gttttgctca acaaactgaa
    32701 tgaagagcaa aaaatgaata tgcgcatcat cagcgccaaa gatcacggtg actctttccg
    32761 caccctggaa agcggtcgtg ccgttgcctt tatgatggat gacgctctgc tggccggtga
    32821 acgtgcgaaa gcgaagaaac cagacaactg ggatatcgtc ggcaagccgc agtctcagga
    32881 agcctacggt tgcatgttgc gtaaagacga tccgcagttc aaaaagctga tggatgacac
    32941 tatcgctcag gtgcagacct ccggtgaagc ggaaaaatgg tttgataaat ggttcaaaaa
    33001 tccaattccg ccgaaaaacc tgaacatgaa tttcgaactg tcagacgaaa tgaaagcact
    33061 gttcaaagaa ccgaatgaca aggcactgaa ctaattacaa gaaccagggg cggaaattcc
    33121 agccctctcg attgttacgt agcacggaca gactatacgc ctgatggtcg ttccccatcg
    33181 ggcctgaaaa ccgcaatacg ctgggtaaca atcttcgagg gtagcagtta acgctgctac
    33241 cctctttttt ctggagtaga tttatgtcta tagactggaa ctggggtatt tttttacaac
    33301 aagccccgtt cggcaacacc acctatcttg gttggatctg gagtggtttt caggtgacga
    33361 tcgctttgtc gatctgcgcc tggattatcg ctttcctcgt cggttcattt ttcggcattt
    33421 tacgtaccgt tccaaaccgt tttctctccg gtctgggtac gttgtatgtc gaactgttcc
    33481 gcaacgtgcc gttgattgtg caattcttta cctggtatct ggtgatcccg gagctgctgc
    33541 cggagaaaat cggcatgtgg tttaaggccg agctggatcc caatattcag tttttccttt
    33601 catccatgct ctgcctgggg ctgtttaccg ccgcccgtgt ttgcgaacag gtacgcgccg
    33661 ctattcagtc gctgccgcgt gggcaaaaga atgccgcgct ggcgatgggc ctgacgctac
    33721 cgcaggctta tcgatacgta ctgctgccta atgcttatcg cgttatcgtc ccgccgatga
    33781 cctcagagat gatgaacctg gtgaaaaact ccgccatcgc ctcgactatt ggtctagtgg
    33841 atatggcggc gcaggcgggt aaattgcttg attactcagc ccacgcctgg gagtcgttta
    33901 ccgccatcac gctggcgtat gtattgatta acgcttttat catgctggtg atgacgctgg
    33961 ttgaacgtaa agttcgcctg cctggcaaca tggggggtaa ataatgtacg agtttgactg
    34021 gagttccatt gtcccttccc tgccatatct gctcgacggg ctggtgatca ccctgaaaat
    34081 caccgtcacg gcagtcgtga ttggtatttt gtggggcacc atgctggcgg tgatgcgctt
    34141 gtccagcttt gcgcctgtag cgtggtttgc caaagcctac gttaacgtat tccgctcgat
    34201 tcctttagtt atggttttgc tgtggtttta cctgattgtg ccgggttttc tgcaaaacgt
    34261 gctgggatta tcaccaaaaa atgatatccg tttaatttcg gcgatggtgg cgttttcgat
    34321 gtttgaagcg gcctactatt cagagattat ccgtgctggt attcaaagta tttctcgtgg
    34381 tcagtcgagc gccgcgctgg cgttgggaat gactcactgg cagtcgatga aactgattat
    34441 tctgccgcag gcgttccgcg cgatggtgcc gctgctgctc actcagggca tcgtactgtt
    34501 ccaggatacc tcgctggtgt atgtgttatc tctggccgat ttcttccgta ccgcctcaac
    34561 cattggtgag cgtgatggta cgcaggttga gatgatcctg tttgccggat ttgtttattt
    34621 cgttattagt cttagcgcgt cgttgttggt cagctacttg aaaagaagga cagcataatg
    34681 attaccctga aaaatgtttc aaaatggtat ggtcactttc aggtgctgac cgactgctca
    34741 accgaagtga aaaaaggcga agtggtggtg gtttgcggcc cgtctggttc cggcaaatca
    34801 acgctgatta aaaccgtcaa cggcctcgaa ccagtgcagc aaggtgaaat caccgtcgat
    34861 ggtatcgtgg ttaatgacaa gaaaaccgat ctggcaaagc tgcgttcccg cgtcgggatg
    34921 gtattccagc atttcgagct gttccctcat ctgtcgatta tcgaaaacct gaccctggcg
    34981 caggtgaaag tgcttaaacg cgataaagcg ccggctcgtg aaaaagcatt gaaattgctg
    35041 gagcgcgtgg gactttccgc acatgccaat aaatttccgg cgcagctttc cggcggtcag
    35101 cagcagcgtg tggcgatcgc tcgcgcattg tgtatggatc ctattgcgat gctgttcgac
    35161 gaaccgacgt cggcgctgga tccggagatg atcaacgaag tgctggacgt gatggtggaa
    35221 ctggcgaacg aaggaatgac catgatggtg gtgacccacg aaatgggctt tgcccgcaaa
    35281 gtggcgaatc gggtgatctt tatggatgag ggtaaaattg tcgaagactc gccgaaagac
    35341 gctttcttcg atgatccgaa atcggaccgc gcaaaagact tcctcgcgaa aatcctgcat
    35401 taatcactct ggcgcgcata cccaagtgcg cgccatctca cgctttatcg tttcgcccct
    35461 cgtatctgtt acatcctggc gttacccttg tcgcaaagaa gcacacaaca aggagcaaca
    35521 atggcactgc caattctgtt agattgcgac ccaggtcatg acgacgctat cgcaatagtt
    35581 ctcgccctcg cctcaccaga gcttgatgtc aaagcaatta cgtcttccgc cggaaaccag
    35641 acaccagaaa aaaccttacg caatgttctt cgtatgctga ccttgctcaa tcgcaccgat
    35701 attccggtag caggcggcgc ggtaaaaccg ttaatgcgtg agttgattat cgcggacaat
    35761 gtgcacggcg aaagcggtct cgacggcccg gcattaccgg aaccggcgtt cgcaccgcaa
    35821 aactgtacgg cggtagagct aatggcgaaa acgctgcgtg aaagtgcgga acctgtcacg
    35881 attgtgtcta ccggaccgca aactaacgtt gccttgctgc tcaatagcca tccggaactg
    35941 catagcaaaa ttgcccgtat cgtgattatg ggcggcgcaa tggggcttgg taactggacg
    36001 cctgcggctg aatttaatat ttacgttgac ccggaagcgg cagaaattgt cttccagtca
    36061 gggatcccgg tggtgatggc tggtctggat gttactcaca aagcacaaat ccacgttgaa
    36121 gacactgagc gtttccgcgc gattggcaac cctgtttcaa ccattgttgc cgaactgctg
    36181 gatttcttcc tcgaatatca taaagacgaa aaatggggct ttgtcggcgc accgctgcat
    36241 gacccatgca ccatcgcctg gctgttgaaa ccggagttgt ttaccactgt tgagcgctgg
    36301 gttggcgtgg aaacacaggg gaaatatact cagggtatga cggttgttga ttattattat
    36361 ctgactggca ataaaccgaa tgccaccgta atggtcgatg ttgatcgtca gggctttgtt
    36421 gatttactgg ctgatcgtct gaaattttac gcttaacaca cgtcattgat taagatcacc
    36481 acgcgttacc tgggtggtct taatctgttt atctcttttt ctttcaagat ataaatgcaa
    36541 tggataatgc agaactcgcc attggtatcg atctcggtac taccaatagt ttaattgccg
    36601 tctggaaaga cggtgccgcg caattaattc caaataagtt cggtgaatat ttaacaccat
    36661 ccataattag catggatgaa aataatcata ttttagtcgg aaaaccggct gtatcacggc
    36721 gtacttcgca tccggataaa acggcagcgt tatttaaacg tgcaatgggc agtaatacca
    36781 actggcggtt aggcagcgac acatttaacg cgccagaact gtcctctttg gtattacgct
    36841 cattaaaaga agatgccgaa gaatttctgc aacgtccgat taaagatgtg gtgatctccg
    36901 ttccggctta tttcagcgat gaacaacgca agcatacccg tttagcagcg gagttagccg
    36961 ggttaaatgc ggtacgctta attaatgaac ccacagcagc tgcgatggcg tatggcctgc
    37021 atacccaaca aaatacccgt tcgctggtgt ttgatctcgg tggcggcacg tttgacgtta
    37081 cggtgcttga gtacgccacg ccggtgattg aagttcacgc ctccgctggc gacaacttcc
    37141 tcggtggcga agattttacc catatgttgg tcgatgacgt tttaaagcgc gcggatgtca
    37201 ccaggaccac gcttaacgag agtgaactgg cagccttgta tgcctgtgtg gaagcggcaa
    37261 aatgtagcaa tcaatcgcca ttacacattc gctggcagta ccaggatgaa gcacgggaat
    37321 gcgaatttta tgagaatgaa ctggaagatt tgtggttgcc gctgctcaat cgcttgcgag
    37381 tgccgattga acaggcgttg cgcgatgcgc gtctgaagcc aagtcaaatc gacagtctgg
    37441 tactggttgg cggcgcgtca caaatgccgc tggtgcagcg aatcgccgtg cgtctgtttg
    37501 gcaaattacc gtatcaaagt tacgacccga gcaccattgt cgcgctgggc gcagcaatcc
    37561 aggccgcctg ccgcttacgc agtgaagata ttgaagaggt gatcctcact gatatttgcc
    37621 cttactcgtt gggcgttgaa gttaaccgcc agggcatttc cggcattttc tcgccaatta
    37681 ttgaacgaaa caccacagta ccggtgtcgc gtgtagaaac ttactcgacc atgcacccgg
    37741 aacaggattc tattacggtt aacgtctatc agggagaaaa ccacaaagtt aaaaataaca
    37801 ttctggttga atccttcgat gtgccgttga agaaaaccgg ggcttatcag tcgattgata
    37861 ttcgctttag ttatgatatc aacggcttgc ttgaagttga cgtgcttctg gaagatggca
    37921 gcgttaagtc cagagtcatt aatcatagcc cggtaacatt gagcgcgcag cagattgaag
    37981 agagtcggac gcggttatcc gcattgaaaa tttatccgcg cgatatgctc atcaaccgca
    38041 cctttaaggc caaactggaa gagttgtggg cgcgggcgct gggtgacgag cgagaagaga
    38101 ttggccgggt gatcaccgat tttgatgcgg cgttgcagtc aaacgatatg gcccgcgtcg
    38161 atgaggtcag gcggcgggcg agcgtttatt tagccattga gacctcataa tcacggctgc
    38221 cggatgcggc gtaaatgctg catccggcat cggcgcaaaa aacacatcgt caactaaaca
    38281 acatcgcgat tttgccgaga gcataaccta taaccagcac cgttaaactg attttgataa
    38341 tgctggtaat gctctgtggt ttttcaccct catcattttt ttcttcatgg ttttcaggtg
    38401 ttggctcatc gacatcttca tcctccggct cgagttttac tttcgtcaac agcatccggg
    38461 tgataatggc ctctaatgga tgagcatctc cgccttccag ccttgtatta agacgggcca
    38521 gcatctcttg tttcggaaac gcttcaagta ctacctgttc cgctacccga tacaaatcca
    38581 gtaacggtga atcttcttct gtctcgctac cgactctcga tttccaccac gcccatgctt
    38641 tgcgtttgaa gaataacgac tcaataaacg attgacctaa cggcgataat tttccgctat
    38701 gtaaaatttc cgcccataac agccgcgtcc gggcgctctc accaggaaca tatttcagcg
    38761 gttgaccttg cgcatctacc atgctccgcg cttcgataac catcactaat aacgcgaaat
    38821 aatccgggca atgacgcttg ctccactgca acaacaaatt atcagcttgt gtagatgggt
    38881 aactttcccg gtacgcgcag agatcggcaa gcaggctttc cccctcgccg cagtacaacc
    38941 tttgagcaca caagtaatat cgggcatctt cgctttctgg ttcttccatt tgccactgtt
    39001 tggctacagg aatgagttcc gccatacctg tttgcaccga gctgtaccag cgcaacagtt
    39061 tgcgatgtaa ctttgcatca tcaggaatga accacggacc ttccatcgcc aaaaacgcag
    39121 tgacgtagtc aggatggtaa cggcagcaac gttccagcgc gaagtaatac tcaacagtct
    39181 ggttctgcaa cgcaacggga tattccagaa ggctaaggaa atcgaaacaa tcaccagcct
    39241 gaatggcctc aagaaaagcc tccctttctt cctcatccat tccttcgctg tcattaaacg
    39301 aatgccagtt caaacgctct gccagcaaag aagcacaatt taatgaaaga tatcgcgctt
    39361 ctatagcgat tgcacacagc ggccagcgta attgatcgac atcctccatg ttccaggtat
    39421 ttaattgctg aataaatttc tgccaggcgg agggctgaaa acgatcactt tccgaatcca
    39481 gtaatgtcct gaatgcacgc agtatttcat gttcagcggc ggcatctttt tcttcatcat
    39541 ctgcttcctc gacaggattt accgccagtc gcagcgcctc ttcataggcc tggcgtaatg
    39601 ctttaaaccc ctgcggatcg gtttcgggat gacataacgg taagcgagca agataagcct
    39661 ggcggataat gtctatttgc gtcgtgcttt caatttcgag gatttgccaa catgttttca
    39721 taggtcgctc acatccttaa gcgttaccgg gcattcaggc tggacagtta atggctgcca
    39781 gggaagcgtg gcatagaaac gatcgcttcc cgctgccagc tcttcgaaca tccgactccc
    39841 ggcgctatcg tatttgtaca gggttagcgc ctcgcgtagc gaagtgttgt cttcgcactg
    39901 agaagattgc cagtagagac gtcctaacga ataggcggaa aaatagtgca tccagctgtg
    39961 atagtaatgg tgagctcgta catagacgcg cgattgcagc cataaactct cttcttccgt
    40021 cagccagttg ttgagcacac cgacccgtga cagaaacccc attcgaacat aatcccaggc
    40081 tttgattccc ccttctccac agcacatagc ggtttgggca acgaattggg tataaatcaa
    40141 ccctctctcc gagccaccag caattaatgc tttccactct tcaggggaag agcgatgcca
    40201 tgtcagatat aagccagcta aatcattggc atgaccatca tcagtcatct gataaatagt
    40261 ctgaattaaa tcatcacgat tatttattcc ccaggatgat gagagatcga attcttttat
    40321 cccggtatac atttttggcg agtcatgcgc gggtttgcaa ccctgattaa gtatttccat
    40381 gggcgcagaa agcgcaaaaa gaagatattg ttcttcctta ttcatttgtt aataatccct
    40441 taagtgcatt aact
//
LOCUS       DBANUU010000029        38825 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000029 DBANUU010000000
VERSION     DBANUU010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.029"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            2..4525
                     /gene="sslE"
                     /locus_tag="ACIE68_001467"
     CDS             2..4525
                     /gene="sslE"
                     /locus_tag="ACIE68_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFX5907805.1"
                     /translation="MLSATLLAGCDGGGSGSSSDTPPVDSGTGSLPEVKPDPTPNPEP
                     TPEPTPDPEPTPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSLRVTGDITCNDESSDGF
                     TFTPGDKVTCVAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDAQELAGSDNKKSNAL
                     SLVTSMNSCPANTEQVCLEFSSVIESKRFDSLYKQIDLAPEEFKKLVNEEVENNAATD
                     KAPSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPSEIILSEGRLVDSQGYGVA
                     GVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKSTIALTELGDEVRGA
                     NIDQLIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLSNGATLDEGEQVVNL
                     PNEFIEQFKTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQIQGVINKLWGVDTN
                     YKSVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNYWLAFGEKRAWD
                     KNELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGEGKLMVIGNPHYNSILRCPNG
                     YSWNGGVNKDGQCTLNSDPDDMKNFMENVLRYLSDDKWTPDAKASMTVGTNLDTVYFK
                     RHGQVTGNSAAFDFHPDFAGISVEHLSSYGDLDPQEMPLLILNGFEYVTQVGNDPYAI
                     PLRADTSKPKLTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESASGFVRLLDAAGLSM
                     ALNKSVVNNDPQGYPNRVRQQRATGIWVYERYPAVDGALPYTIDSKTGEVKWKYQVEN
                     KPDDKPKLEVASWLEDVDGKQETRYAFIDEADHKTEDSLKAAKEKIFAAFPGLKECTN
                     PAYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQAADLGTNIQRLYQH
                     ELYFRTNGRKGERLSSVDLERLYQNMSVWLWNDTSYRYEEGKNDELGFKTFTEFLNCY
                     ANDAYAGGTKCSADLKKSLVDNNMIYGDGSSKAGMMNPSYPLNYMEKPLTRLMLGRSW
                     WDLNIKVDVEKYPGAVSEEGQNVTETISLYSNPTKWFAGNMQSTGLWAPAQKEVTIKS
                     NANVPVTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTVKFKVPYGGLIYIKG
                     NSSTNESASFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESDAFVYTTPKKNLNASNY
                     TGGLEQFANDLDTFASSMNDFYGRDETSGKHRMFTYKNLTGHKHRFTNDVQISIGDAH
                     SGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGATEVANNVLALYMQ
                     DRYLGKMNRVADDITVAPEYLEESNGQAWARGGAGDRLLMYAQLKEWAEKNFDIKKWY
                     PEGELPKFFSDREGMKGWNLFQLMHRKARGDDVGDKTFGGKNYCAESNGNAADTLMLC
                     ASWVAQTDLSEFFKKWNPGANAYQLPGASEMSFEGGVSQSAYNTLASLKLPKPEQGPE
                     TINKVTEHKMSVE"
     gene            4673..5482
                     /gene="pppA"
                     /locus_tag="ACIE68_001468"
     CDS             4673..5482
                     /gene="pppA"
                     /locus_tag="ACIE68_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFX5907806.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFIAFYSLRWIAGVVLRKEALGMGDVLLFAALGSW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATIYLQALF"
     gene            5548..5958
                     /gene="gspS2"
                     /locus_tag="ACIE68_001469"
     CDS             5548..5958
                     /gene="gspS2"
                     /locus_tag="ACIE68_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFX5907807.1"
                     /translation="MSIKQMPRRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            5976..6935
                     /gene="gspC"
                     /locus_tag="ACIE68_001470"
     CDS             5976..6935
                     /gene="gspC"
                     /locus_tag="ACIE68_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFX5907808.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSFNTDKEHLRKIVRGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGETLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEGHSTVAVTNKKAVSDEAKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            6965..9025
                     /gene="gspD"
                     /locus_tag="ACIE68_001471"
     CDS             6965..9025
                     /gene="gspD"
                     /locus_tag="ACIE68_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFX5907809.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREIVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            9025..10518
                     /gene="gspE"
                     /locus_tag="ACIE68_001472"
     CDS             9025..10518
                     /gene="gspE"
                     /locus_tag="ACIE68_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFX5907810.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            10518..11741
                     /gene="gspF"
                     /locus_tag="ACIE68_001473"
     CDS             10518..11741
                     /gene="gspF"
                     /locus_tag="ACIE68_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFX5907811.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDALQASGVYWLAGLLGLLVLGQRLLKNPAMRLRWDQTVLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            11758..12213
                     /gene="gspG"
                     /locus_tag="ACIE68_001474"
     CDS             11758..12213
                     /gene="gspG"
                     /locus_tag="ACIE68_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFX5907812.1"
                     /translation="MYSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            12217..12780
                     /gene="gspH"
                     /locus_tag="ACIE68_001475"
     CDS             12217..12780
                     /gene="gspH"
                     /locus_tag="ACIE68_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFX5907813.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASSGVVQTFATDSEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDAPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            12777..13148
                     /gene="gspI"
                     /locus_tag="ACIE68_001476"
     CDS             12777..13148
                     /gene="gspI"
                     /locus_tag="ACIE68_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFX5907814.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRQQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSLH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            13145..13744
                     /gene="gspJ"
                     /locus_tag="ACIE68_001477"
     CDS             13145..13744
                     /gene="gspJ"
                     /locus_tag="ACIE68_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001691118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFX5907815.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            13747..14724
                     /gene="gspK"
                     /locus_tag="ACIE68_001478"
     CDS             13747..14724
                     /gene="gspK"
                     /locus_tag="ACIE68_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFX5907816.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            14721..15899
                     /gene="gspL"
                     /locus_tag="ACIE68_001479"
     CDS             14721..15899
                     /gene="gspL"
                     /locus_tag="ACIE68_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFX5907817.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPVVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADFVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            15901..16437
                     /gene="yghD"
                     /locus_tag="ACIE68_001480"
     CDS             15901..16437
                     /gene="yghD"
                     /locus_tag="ACIE68_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFX5907818.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETILQQLVAMNTRLKSAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIRRIAERGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKPGMVNVQRLEFGRG"
     gene            complement(16586..16660)
                     /locus_tag="ACIE68_001481"
     tRNA            complement(16586..16660)
                     /locus_tag="ACIE68_001481"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16625..16627),aa:Phe,seq:gaa)
     gene            complement(16766..17473)
                     /gene="yqgA"
                     /locus_tag="ACIE68_001482"
     CDS             complement(16766..17473)
                     /gene="yqgA"
                     /locus_tag="ACIE68_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFX5907819.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            17626..17709
                     /gene="yqgH"
                     /locus_tag="ACIE68_001483"
     CDS             17626..17709
                     /gene="yqgH"
                     /locus_tag="ACIE68_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFX5907820.1"
                     /translation="MHATSLHSLAYRRFARNVSLSMLLLMR"
     gene            17871..20006
                     /gene="speC"
                     /locus_tag="ACIE68_001484"
     CDS             17871..20006
                     /gene="speC"
                     /locus_tag="ACIE68_001484"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFX5907821.1"
                     /translation="MKSMNIAASSDLVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTMRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(20056..21312)
                     /gene="nupG"
                     /locus_tag="ACIE68_001485"
     CDS             complement(20056..21312)
                     /gene="nupG"
                     /locus_tag="ACIE68_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFX5907822.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(21514..22593)
                     /gene="mltC"
                     /locus_tag="ACIE68_001486"
     CDS             complement(21514..22593)
                     /gene="mltC"
                     /locus_tag="ACIE68_001486"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFX5907823.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(22658..22933)
                     /gene="yggX"
                     /locus_tag="ACIE68_001487"
     CDS             complement(22658..22933)
                     /gene="yggX"
                     /locus_tag="ACIE68_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFX5907824.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(22961..24013)
                     /gene="mutY"
                     /locus_tag="ACIE68_001488"
     CDS             complement(22961..24013)
                     /gene="mutY"
                     /locus_tag="ACIE68_001488"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFX5907825.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            24174..24893
                     /gene="trmB"
                     /locus_tag="ACIE68_001489"
     CDS             24174..24893
                     /gene="trmB"
                     /locus_tag="ACIE68_001489"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFX5907826.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            24893..25219
                     /gene="yggL"
                     /locus_tag="ACIE68_001490"
     CDS             24893..25219
                     /gene="yggL"
                     /locus_tag="ACIE68_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFX5907827.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            25403..26122
                     /gene="yggN"
                     /locus_tag="ACIE68_001491"
     CDS             25403..26122
                     /gene="yggN"
                     /locus_tag="ACIE68_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFX5907828.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            26298..27344
                     /gene="ansB"
                     /locus_tag="ACIE68_001492"
     CDS             26298..27344
                     /gene="ansB"
                     /locus_tag="ACIE68_001492"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFX5907829.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            27461..28468
                     /gene="yggM"
                     /locus_tag="ACIE68_001493"
     CDS             27461..28468
                     /gene="yggM"
                     /locus_tag="ACIE68_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFX5907830.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(28623..29759)
                     /gene="hemW"
                     /locus_tag="ACIE68_001494"
     CDS             complement(28623..29759)
                     /gene="hemW"
                     /locus_tag="ACIE68_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFX5907831.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(29752..30345)
                     /gene="rdgB"
                     /locus_tag="ACIE68_001495"
     CDS             complement(29752..30345)
                     /gene="rdgB"
                     /locus_tag="ACIE68_001495"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFX5907832.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(30353..30643)
                     /gene="yggU"
                     /locus_tag="ACIE68_001496"
     CDS             complement(30353..30643)
                     /gene="yggU"
                     /locus_tag="ACIE68_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFX5907833.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSLVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(30640..31206)
                     /gene="yggT"
                     /locus_tag="ACIE68_001497"
     CDS             complement(30640..31206)
                     /gene="yggT"
                     /locus_tag="ACIE68_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFX5907834.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(31224..31928)
                     /gene="yggS"
                     /locus_tag="ACIE68_001498"
     CDS             complement(31224..31928)
                     /gene="yggS"
                     /locus_tag="ACIE68_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFX5907835.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            31946..32926
                     /gene="yggR"
                     /locus_tag="ACIE68_001499"
     CDS             31946..32926
                     /gene="yggR"
                     /locus_tag="ACIE68_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFX5907836.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGCVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            complement(33089..33505)
                     /gene="ruvX"
                     /locus_tag="ACIE68_001500"
     CDS             complement(33089..33505)
                     /gene="ruvX"
                     /locus_tag="ACIE68_001500"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFX5907837.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(33505..34068)
                     /gene="yqgE"
                     /locus_tag="ACIE68_001501"
     CDS             complement(33505..34068)
                     /gene="yqgE"
                     /locus_tag="ACIE68_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFX5907838.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(34177..35127)
                     /gene="gshB"
                     /locus_tag="ACIE68_001502"
     CDS             complement(34177..35127)
                     /gene="gshB"
                     /locus_tag="ACIE68_001502"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFX5907839.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(35140..35871)
                     /gene="rsmE"
                     /locus_tag="ACIE68_001503"
     CDS             complement(35140..35871)
                     /gene="rsmE"
                     /locus_tag="ACIE68_001503"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFX5907840.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(35951..36658)
                     /gene="endA"
                     /locus_tag="ACIE68_001504"
     CDS             complement(35951..36658)
                     /gene="endA"
                     /locus_tag="ACIE68_001504"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFX5907841.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(36753..37250)
                     /gene="yggI"
                     /locus_tag="ACIE68_001505"
     CDS             complement(36753..37250)
                     /gene="yggI"
                     /locus_tag="ACIE68_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFX5907842.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(37327..38721)
                     /gene="galP"
                     /locus_tag="ACIE68_001506"
     CDS             complement(37327..38721)
                     /gene="galP"
                     /locus_tag="ACIE68_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFX5907843.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         9454 a         9857 c        10630 g         8884 t
ORIGIN      
        1 tattttgagt gcaaccctgt tagccggttg tgatggcggt ggctccggat cttcctccga
       61 tacgccgcct gtagattctg gaacagggtc tttgccggaa gtgaaacctg atccaacacc
      121 aaacccggag ccgacgcctg agccaacgcc ggacccagaa cctacgccgg aaccgacacc
      181 tgatcctgag ccaacaccag aaccggagcc agaacctgtt cctacgaaaa cgggttatct
      241 gaccctgggc ggaagcctgc gggtaactgg tgatatcacc tgtaatgatg aatccagcga
      301 tggctttacc tttacaccag gcgacaaagt cacctgtgtg gcagggaaca acacgacaat
      361 tgctaccttc gacacccagt cagaagctgc gcgtagcctg cgtgcggttg aaaaagtgtc
      421 gtttagtctt gaggacgcgc aagaactggc gggttccgac aacaagaaaa gcaatgcgct
      481 ctcgctggtc acctccatga acagttgccc ggcgaataca gaacaggtgt gcctggagtt
      541 ctcctcggtg atcgagagta aacgtttcga ctcgctgtat aagcaaatcg atctggcacc
      601 ggaagagttc aaaaagctgg tcaatgaaga ggtggaaaac aatgccgcga ctgataaagc
      661 gccatccact catacttcac cggtagtccc cgtcacgact ccgggaacaa aaccggatct
      721 gaacgcctcc ttcgtgtcgg ctaacgcgga acagttttat cagtatcaac cttcggaaat
      781 cattctctca gaaggtcgcc tggtggatag ccagggatat ggtgttgctg gcgtcaacta
      841 ctacaccaat tcaggccgtg gcgtgacagg ggaaaatggt gaattttctt ttagctgggg
      901 cgaaaccatc tcctttggta tcgatacctt tgaactgggt tcagtgcgcg gcaataagtc
      961 gaccattgcg ctgactgaac tgggtgatga agttcgcggg gcgaatatcg atcagcttat
     1021 tcatcgctat tcgacgaccg ggcaaaataa tacccgtgtt gttccggatg atgtacgcaa
     1081 ggtctttgcc gaatatccca acgtgatcaa cgagattatc aatctctcgt tatccaacgg
     1141 tgcgacgctg gatgaaggtg agcaagttgt taatctgcca aacgaattta tcgagcagtt
     1201 taagacgggt caggccaaag agatcgatac cgcgatttgt gcgaaaaccg acggttgtaa
     1261 cgaggctcgc tggttctcgc tgacaacgcg caatgttaat gacggccaga ttcagggcgt
     1321 tatcaacaag ctgtggggcg tggatacgaa ctacaaatct gtcagtaagt tccatgtatt
     1381 ccatgactct accaacttct atggcagcac gggtaatgcg cgcggtcagg cggtggtgaa
     1441 tatctccaac gcggccttcc cgattctgat ggcgcgtaat gataaaaact actggctggc
     1501 gtttggcgaa aaacgcgcct gggataaaaa tgagctggcg tacattacgg aagcgccttc
     1561 tcttgttgag ccggaaaacg ttacgcgcga taccgccact ttcaacctgc cgtttatttc
     1621 gctggggcaa gtcggtgagg gcaaactgat ggttatcggt aacccacact acaacagcat
     1681 tttgcgttgc ccgaacggtt acagctggaa cgggggcgtt aataaagatg ggcagtgtac
     1741 gctcaacagc gacccggatg acatgaagaa cttcatggag aacgtgctgc gctatctgtc
     1801 cgacgataaa tggacgccgg acgcgaaagc cagcatgacc gtaggcacca acctggatac
     1861 tgtctatttt aaacgtcatg gtcaggttac aggaaacagc gctgcgttcg acttccatcc
     1921 ggattttgcg ggcatctctg ttgagcattt aagtagctat ggcgatctcg atccgcagga
     1981 aatgccgctg ctgatcctta acggctttga atatgtgact caggtgggta acgatcctta
     2041 tgcaatcccg ctgcgtgcag ataccagcaa accgaagctg actcagcagg atgtgaccga
     2101 tctgatcgcc tatctgaaca aaggtggatc ggtgctgatc atggaaaacg tgatgagcaa
     2161 tcttaaggaa gagagcgcgt ctggttttgt gcgtctgttg gatgccgcag gtctgtcgat
     2221 ggcactgaac aagtcggtag taaataacga tccgcaaggg tatccgaacc gcgttcgtca
     2281 gcagcgcgca acgggcattt gggtctatga acgttatcct gccgtagatg gtgcgctgcc
     2341 gtacaccatc gatagtaaga caggggaagt taagtggaaa tatcaggtag aaaacaaacc
     2401 tgatgacaaa ccgaagctgg aagttgccag ctggctggaa gatgtagatg gcaaacagga
     2461 aacgcgttat gcctttattg atgaggccga tcataaaaca gaggattctc tgaaggctgc
     2521 gaaggagaaa attttcgccg cgttcccggg gctgaaagag tgtactaatc cggcatatca
     2581 ctatgaggtc aactgcctgg aatatcgtcc tggcacgggg gttccggtta ctggtggcat
     2641 gtatgttcca cagtatacgc aactaagcct taacgccgac actgcaaaag cgatggtgca
     2701 ggctgcggat ttaggcacca acattcagcg tctgtatcag catgagctct acttccggac
     2761 caatggtcgc aaaggtgagc gtctgagcag cgtcgatctg gaacgtctgt accagaacat
     2821 gtcggtctgg ctgtggaacg atacgagcta tcgttatgaa gaaggcaaaa atgacgagct
     2881 gggctttaaa acgttcaccg agttcctgaa ctgctacgcc aatgatgcct atgcaggcgg
     2941 caccaagtgt tctgcagatc tgaaaaaatc gctggtcgat aacaacatga tctacggtga
     3001 cggtagcagc aaagcgggca tgatgaaccc gagctacccg ctcaactata tggaaaaacc
     3061 gctgacacgc ctgatgctgg gccgttcctg gtgggatctg aacatcaaag ttgatgtcga
     3121 gaagtatccg ggagcagtat cggaagaggg acagaacgtt actgaaacca tcagcctgta
     3181 ctcgaatccg accaaatggt ttgcaggtaa catgcagtca actggcctgt gggcaccggc
     3241 tcagaaagag gtcaccatta agtccaatgc gaacgttcct gtgaccgtca ccgtggcgct
     3301 ggctgacgac ctgaccggac gtgagaagca tgaagttgcg ctgaaccgtc cgccaagagt
     3361 gactaaaacg tactctctgg acgctagcgg tacggtgaag ttcaaggtgc cttacggtgg
     3421 cctgatttat atcaagggca atagctctac caatgaatct gccagcttca cctttactgg
     3481 cgtggtaaaa gcaccgttct ataaagacgg cgcatggaaa aacgatctga actcaccggc
     3541 tccgctgggt gagctggaat cagacgcttt cgtctatacc acaccgaaga agaacctgaa
     3601 tgccagcaat tacactggcg gactggagca attcgctaac gatctggaca cctttgccag
     3661 ctcgatgaat gacttctacg gtcgtgatga aacgagcggt aagcaccgga tgtttaccta
     3721 taaaaacttg acgggacata aacatcgttt cacaaacgat gtgcagatct ccatcggtga
     3781 tgcgcactcg ggttatccgg taatgaacag cagcttctcg acgaacagca ccacgctgcc
     3841 gacgacgccg ctgaacgact ggctgatctg gcacgaagta gggcacaacg ccgcagaaac
     3901 gccgctgact gttccaggcg cgaccgaagt ggcgaacaac gtgctggcgc tgtacatgca
     3961 ggatcgttat ctcggcaaga tgaaccgtgt cgctgacgat attaccgtcg caccggaata
     4021 tctggaggag agcaacggtc aggcatgggc gcgcggcggt gcgggtgacc gtctgctgat
     4081 gtacgcgcag ctgaaggaat gggcagagaa aaactttgat atcaagaaat ggtatccaga
     4141 aggtgaattg ccgaagttct tcagcgatcg tgaagggatg aaaggctgga acctgttcca
     4201 gttgatgcac cgtaaagcgc gcggcgatga cgttggtgac aagacctttg gtggcaagaa
     4261 ttactgtgct gaatccaacg gtaacgctgc ggacacgctg atgctgtgtg cctcctgggt
     4321 cgcccagacg gatctttcgg agttctttaa gaaatggaat ccgggtgcga atgcttacca
     4381 gctgccgggg gcgagcgaga tgagcttcga gggcggtgtg agccagtcgg cgtacaacac
     4441 gctggcgtca ctcaagctgc cgaaaccgga acagggaccg gaaaccatta acaaggttac
     4501 cgagcataag atgtctgtcg agtaatcctg tatgccggat aaggcgttca cgccgcatcc
     4561 ggcatgaaat aaggcgcacc gggttgcgcc agcttatcgg gcctggagga aagccgtatg
     4621 gcatacactt gcaggcccgc atccggcaag ttacaacaaa caacctttaa ccatgctttt
     4681 tgatgttttt cagcaatacc ccgcggcgat gcccatactg gcaaccgtcg gaggattgat
     4741 catcggcagt tttttgaatg tggtgatttg gcgttacccc atcatgctgc gccaacaaat
     4801 ggcggagttt catggtgaaa tgccgagtgc gcagtcaaaa ataagccttg cgctaccgcg
     4861 ttcccactgc ccacattgtc agcagaccat ccggatacgt gacaatattc cgctgctctc
     4921 ctggctgatg ctcaaagggc gctgccgtga ttgtcaggcg aaaatcagca agcgttatcc
     4981 gctggtggag ttattgacgg cactcgcttt tttgctggcg agtctggtct ggccggaaag
     5041 tggatgggcg ctggcggtga tgatattatc cgcctggctg attgccgcga gcgtcattga
     5101 cctcgatcac caatggctgc ccgatgtttt tactcagggc gtattgtgga cgggactgat
     5161 tgcggcatgg gcgcagcaga gcccgctcac gctacaagat gcagttaccg gcgtcctggt
     5221 ggggtttatc gctttttact ccctgcgctg gatagccgga gtagttctgc gtaaagaagc
     5281 attaggcatg ggcgatgtat tattgttcgc tgcgttaggt agttgggtgg ggccgttgtc
     5341 gctacccaat gttgctttaa tcgcatcatg ctgcggcctg atatatgccg ttattacaaa
     5401 aagaggatca accacactgc cttttggacc gtgtttaagt ctgggcggta tagcaacaat
     5461 ttatctacag gcattgtttt aatgataaca acgtcattat caaagtgaca ttttaactct
     5521 tattaataac cttagagatt atttaccatg tcgataaaac aaatgccaag gagggtatta
     5581 atatcgctat tgttgagcgt tacaggatta ttaagtggct gtgccagcca taatgaaaat
     5641 gccagtttac tggcgaaaaa acaggcgcaa aatatcagcc aaaacctgcc gattaaatct
     5701 gcgggatata ccttagtgct ggcacaaagt agcggcacga cggtaaaaat gaccattatc
     5761 agcgaagcgg ggactcagac cacgcagacg cctgacgcct ttttaaccag ctatcaacga
     5821 caaatgtgcg ctgacccaac ggtgaaatta atgattaccg agggaattaa ttacagcata
     5881 acgattaatg atacacgtac aggtaaccag tatcagcgga aactggatcg taccacctgt
     5941 ggaatagtca aagcataacg tcgggtagat ataaattggc gcgggttgtt tttcgtgacg
     6001 cacgaattta tctcattcaa tggctgacaa aaattcgtca cactcttaac cagagacaat
     6061 cttttaatac agacaaagag catctgcgaa aaattgtacg cgggatgttc tggctgatgc
     6121 tgcttattat ttctgcaaaa gtggcgcatt cactctggcg ctatttctcc ttttctgcgg
     6181 aatatacggc tgtttcccca tcggcgaata aaccgccccg tgcggatgca aaaacgttcg
     6241 ataaaaatga cgtgcaatta atcagccagc aaaactggtt tggcaaatat cagcccgtcg
     6301 ccacgccggt aaaacaaccc gaacctgtgc ctgtggcaga aacgcgtctt aatgtggtgc
     6361 tgcgcgggat tgcctttggt gccagacccg gcgcggttat tgaagaaggc ggtaaacagc
     6421 aggtctattt gcagggtgaa acgcttggct cgcacaatgc ggtgattgag gaaatcaatc
     6481 gcgaccatgt gatgctgcgc tatcagggaa aaatagagcg cctgagcctg gcagaagagg
     6541 ggcattccac cgtagccgtg accaacaaaa aagctgtcag tgacgaagca aagcaagctg
     6601 ttgctgaacc tgctgtcagt gcgccagtgg agatcccgac tgccgtgcgt caggcactgg
     6661 cgaaagatcc gcagaaaatt tttaactata tccagcttac gcctgtgcgt aaggagggaa
     6721 ttgtcggtta tgcagtgaag ccgggggcag atcgttctct gttcgatgcc agcggtttca
     6781 aggaaggcga tatcgccatt gcgctaaatc agcaggattt cactgatcca cgagcaatga
     6841 ttgctctgat gcggcagtta ccttcaatgg attccattca acttacggtt ttacgcaagg
     6901 gtgcgcgcca cgacatttcc atcgcgctgc gctaaccgca tttaatccag gagaatcatt
     6961 catcgtgttt tggcgtgata ttacgttgtc ggtctggcgt aagaagacaa ctggcctcaa
     7021 aacaaaaaag cgtttactgc cgctggtgct ggcagcggca ttatgcagtt caccggtctg
     7081 ggcggaagaa gccactttca ccgctaattt taaagatacc gacctgaaat cgttcatcga
     7141 aaccgtcggc gctaacctta ataaaaccat cattatgggg ccgggcgtac aggggaaagt
     7201 gagtattcgc accatgaccc cgctcaatga acgccagtat taccagctat tccttaacct
     7261 gctggaagca caggggtatg ccgtcgtacc gatggaaaac gacgtgctga aggtggtgaa
     7321 atccagcgct gcgaaagtcg agccgctgcc gctggtcggt gaaggcagcg acaactacgc
     7381 gggcgatgaa atggtcacca aagtagtgcc ggtacgtaat gtgtcggtac gcgagttggc
     7441 accgattctg cgccagatga tcgacagcgc aggctcaggc aacgttgtta attacgatcc
     7501 ctccaacgtg attatgctca ccggacgcgc ctccgttgtg gagcgcctga cagaagtgat
     7561 ccagcgcgtg gatcacgcgg gtaatcgcac cgaagaggtg atcccgctgg ataacgcctc
     7621 ggcttcggaa atcgcccgcg tgctggaaag cctgacgaaa aacagcggcg agaaccagcc
     7681 agcgacgctg aaatctcaaa ttgtcgccga cgaacgcacc aacagtgtga ttgtcagtgg
     7741 tgacccggcc acgcgggaca aaatgcgccg cctgatccgt cggctggact cagaaatgga
     7801 acgcagcggc aacagccagg ttttctatct caaatacagc aaagccgaag atctggtcga
     7861 tgtgctgaag caggtcagcg gcacgctcac ggcggctaaa gaagaggcgg aaggcacggt
     7921 cggtagcggg cgtgagattg tatccatcgc cgccagcaaa cacagtaatg ccctgattgt
     7981 aaccgcaccg caggacatca tgcagtcgct gcaaagcgtg attgaacaac tggatattcg
     8041 ccgtgctcag gtgcacgtcg aggcgttgat cgtggaagtt gccgaaggta gcaatatcaa
     8101 cttcggcgtg cagtgggcgt cgaaagatgc cggattaatg cagtttgcta atggtacgca
     8161 gatccctatc ggtacgctgg gggcagccat ttctcaggcg aaaccgcaga aaggctcgac
     8221 ggtgatcagt gaaaacggcg ctaccaccat taatccggat accaacggcg atctctccac
     8281 gctcgcccag cttctttccg gctttagcgg tacggcggtt ggtgtggtga aaggcgactg
     8341 gatggcgctg gtgcaggcgg ttaaaaacga ctccagctcg aacgtgctct ccacgccgag
     8401 catcaccacg ctggacaacc aggaagcctt cttcatggtg ggccaggacg ttccggtatt
     8461 aaccggctct accgttggct ccaataacag caatcctttc aatacagtag aaaggaaaaa
     8521 agtcggcatc atgctgaaag tcacgccgca gattaacgaa ggtaacgcgg tacagatggt
     8581 gattgagcag gaagtctcga aggtggaagg acagaccagc ctcgacgtgg tgtttggtga
     8641 gcgcaaactg aaaaccaccg tgctggctaa cgatggtgag ctgattgtgc ttggtggtct
     8701 gatggacgat caggcgggag aaagcgtggc gaaagtgccg ctgctgggcg atatcccatt
     8761 gattggtaac ctgtttaaat cgacggcgga taaaaaagaa aaacgtaacc tgatggtgtt
     8821 tatccgcccg accattctgc gtgacggtat ggcggcagac ggcgtgtcgc agcgcaaata
     8881 taactatatg cgcgccgaac agatctatcg tgatgagcaa ggtttaagcc tgatgccgca
     8941 caccgcacag ccggtactgc cagcgcaaaa ccaggcctta ccgccggaag ttcgcgcgtt
     9001 cctcaatgcc gggagaacgc gttaatggtg cctgtagcac aggaaaccac cgccaacacc
     9061 gtgcgtctgc cctacagttt cagccgtcgg tttagcctgg tggcatggtg cgaagcgtcg
     9121 ctggagatcc tccacgttca tccgctatcg ctctctgttt tgcaggagct gcagcggggg
     9181 ctgaacgcgc cctttacgct gcggcaaatc gacgaggccg aatttgaaca gcggctgaat
     9241 gcggtctggc agcgggactc ttccgaggct cgccagctga tggaagatct cggttctgct
     9301 gaggactttt ttactctcgc ggaagaactg ccagaaacgg aagatctgct ggaaagcgac
     9361 gacgatgcgc cgatcatcaa actgatcaac gccatgctgg cagaggcaat caaagaaggc
     9421 gcttcggata tccacattga gacgtttgaa aagagtctgg tgatccgttt tcgtgttgac
     9481 ggcacattac atgaaatgct gcgtccgggg cgcaaactgg cctcgctgct ggtgtcgcgt
     9541 atcaaggtga tggcgcggct ggacattgcc gaaaagcgcg tgccgcagga tggacgtatt
     9601 gcgctgttgc tgggcggccg ggcgattgac gtgcgtgtct ccaccatgcc ttctgcctgg
     9661 ggcgagcgcg tggtgctgcg actgctggac aaaaaccagg cccgcctgac gctggagcgt
     9721 ctggggctta gccagcaact gaccgcgcag ttgcgccagc tgttacacaa accgcacggc
     9781 atctttctgg tgacggggcc gacgggttcc ggcaaaagca ccacgctgta cgccggattg
     9841 caggagctga acaaccattc gcgcaacatt ctcacggttg aagatcccat cgaatacatg
     9901 attgaaggga tcggtcagac gcaggttaac acccgcgtcg gcatgacctt cgcccgtggc
     9961 ctgcgcgcga ttttgcgtca ggacccggat gtggtgatgg tcggtgaaat ccgcgatacc
    10021 gaaaccgcag aaatcgccgt ccaggcttca cttaccggac acctggtcct ttccacgctg
    10081 cataccaaca cagcggtggg ggcgatcaca cgtttgcagg atatgggcgt ggagcctttc
    10141 ctgctctctt ccagtctgac gggcgtgatg gcgcagcgac tggttcgcac gctgtgtccc
    10201 gactgccgcc agcccgcacc agccactgac gaagaaaaac gcctgctggg aattaccgac
    10261 gcccgtaccg tcactctgta ccatccacag ggctgtcccg cctgtaatca caaaggtttt
    10321 cgcggacgga ctgccatcca tgagctgatc gtggtggatg ccacattgcg tgatttgatc
    10381 caccgtcagg ccggggagct ggagctggaa cgttatgtcc gacaacactc tgcgggtatc
    10441 cgcagcaacg gcattgagaa agtgctcgcc ggagaaacct ctctcgatga agttctgcgg
    10501 gtaaccatgg aggcgtaatg gcactgtttt actatcaggc gctggagcgt aatggtcgca
    10561 aaaccaaagg tatgattgag gcggattccg cgcgtcatgc ccgccagttg ttgcgcggta
    10621 aagagcttat ccccgtgcat attgaagccc ggatgaatac ttcgtcaggg gggatgttgc
    10681 agcgtcggcg gcacgcacat cgtcgcgtgg cggcggcaga tctggcgctg ttcacgcgcc
    10741 aactggcaac gttagtacag gcagcaatgc cgctggaaac ctgcttacag gcggtaagtg
    10801 agcaaagtga aaaactgcat gtaaaaagcc tcggaatggc gctgcgcagc cggattcagg
    10861 aaggttacac cctgtcggac agcctgcgcg aacatccccg cgtctttgat tccctgtttt
    10921 gttcgatggt tgctgccgga gaaaaatccg ggcatctcga cgtggtgctc aatcgcctgg
    10981 cagattacac cgaacagcga cagcgcctga aatcacgtct gttgcaggcc atgctctatc
    11041 cgctggttct gctggtggtg gcaacgggcg tagtcactat tttgctgacg gcagtggtgc
    11101 cgaaaatcat cgaacagttt gatcatctcg gacacgcgct gcccgcctcc acccgcacgc
    11161 ttattgccat gagcgacgcg ttacaggcca gcggcgttta ctggctggcg ggtttgctgg
    11221 ggcttctggt gctggggcaa cggctactta aaaatcctgc tatgcgcctg cgctgggatc
    11281 aaaccgtgct gcgcctgcct gtgacggggc gtgttgcgcg cgggctgaat acggcgcgtt
    11341 tttcccgcac cttaagcatc ctcaccgcca gcagtgttcc gctactggaa ggtattcaga
    11401 ccgctgccgc cgtgtcggca aatcgctatg tcgaacaaca actactgctg gcggcagatc
    11461 gcgtccgcga aggaagcagt ctgcgcgccg cgctggcgga gttgcgcctg ttcccgccga
    11521 tgatgctgta catgatcgcc tccggcgaac agagcggcga actggaaacc atgcttgagc
    11581 aggccgcagt caaccaggaa cgggaatttg atacccaggt ggggctggcg ttagggctgt
    11641 ttgaaccggc gctggtggtg atgatggcgg gcgtggtgct gtttatcgtc atcgccatcc
    11701 ttgagccgat gctgcaactg aacaatatgg ttggaatgta atttacggag ttatcacatg
    11761 tattcgttat cccgcacaca aaaaccacgg gcaggtttta ccctgctgga agtgatggtg
    11821 gtgattgtta ttcttggcgt cctggcaagt ctggtggtgc ctaacctgtt gggcaataaa
    11881 gagaaagcgg atcggcaaaa agccatcagc gatatcgtgg cgctggagaa tgcgctggat
    11941 atgtaccgac tggataacgg gcgttatccg accacagaac aggggcttga ggcgctgatc
    12001 cagcagccag ccaatatggc ggactcccgc aactaccgta ctggtggata catcaaacga
    12061 ctgccaaagg atccgtgggg caatgattat cagtatctca gcccgggtga aaaaggactg
    12121 tttgatgttt ataccctggg ggcggatggt caggaaaacg gggaaggcgc tggcgcagat
    12181 atcggtaact ggaatttgca ggagtttcag taagcagtgc ctgaacgcgg attcacactt
    12241 ctggaaatca tgttggtgat tttccttatc ggccttgcca gttcgggcgt ggtacagacg
    12301 tttgcgaccg attctgaacc gcccgcgaaa aaagcggcac aggattttct gactcgcttt
    12361 gcgcagttta aggacagggc agtgattgaa gggcagacac tcggtgtgct aatcgacgcg
    12421 cctggctatc agtttatgca gcgtcgtcag gggcagtggt tgcccgtttc cgcgacccgg
    12481 ttatcggcac aggttacggt gccaaaacag gtgcagatgc tgttacaacc cggaagtgat
    12541 atctggcaga aggagtatgc gctggagctg caacgtcgtc gcctgacgct gcacgatatt
    12601 gaactggagc tgcaaaaaga ggcgaaaaag aagacgccgc agatccgttt ttcgcctttt
    12661 gaacccgcca cgccgtttac gctgcgcttc tactcggcgg cgcaaaacgc atgttgggcg
    12721 gtaaaactgg cgcacgatgg cgcgttatcc ctcaatcaat gtgatgagag gatgccatga
    12781 agcgcggatt taccttgctg gaagtgatgc tcgcgctggc gatttttgcg ctggctgcca
    12841 cggcggtgtt acagattgcc agcggtgcgc tgagtaatca gcaaattctt gaggaaaaaa
    12901 cggtagcggg ctgggtggct gaaaaccaga ccgcgctgct ctacctgatg acccgcgagc
    12961 agcgggcggt caggcagcag ggcgagagcg atatggcagg aagccgctgg tactggcgaa
    13021 ccacaccgct gagtacaggc aacgcgctgc tccaggcggt ggatattgaa gtcagccttc
    13081 acgaagactt ttcgtcggtg attcagtcac gacgcgcctg gtttagcgcc gtgggaggcc
    13141 aacagtgaga aggactcgcg ctggtttcac gttactggaa atgttggtgg caattgccat
    13201 ttttgcctcg ctggcactga tggcgcagca ggtgactaac ggcgtcacgc gcgtgaatag
    13261 cgccgtcgcc ggacacgatc aaaaactaaa cctcatgcag caaacgatga gttttctgac
    13321 ccacgatctg acccaaatga tgccgcgtcc ggtaagaggc gatcagggcc agcgtgaacc
    13381 agcgttactg gctggcgctg gcgtgctggc gtctgagagt gaaggaatgc gttttgtgcg
    13441 cggcggtgtg gttaacccgt tgatgcgtct gccgcgcagt aatctgctca ccgtcggtta
    13501 ccgcattcat gacggttatc tcgaacggtt agcctggccg ctgaccgatg ccgcaggcag
    13561 cgtgaagccg acaatgcaaa agttgattcc ggccgattcg ctccgtttgc agttctacga
    13621 cggcacgcgc tggcaggaga gctggtcgtc agtacaggcg atccctgtgg cggtacgcat
    13681 gaccctgcat tcgccgcaat ggggcgagat tgaacgcatc tggttgttac gcgggccgca
    13741 ataatcatga tcacctcacc accaaaacgc ggaatggcac tggttgtggt gctggtattg
    13801 ctggcagtta tgatgctggt aaccatcacg ctttccgggc ggatgcagca gcaactggga
    13861 agaacgcgca gccagcagga gtaccagcag gcgctgtggt acagcgccag tgcagaaagc
    13921 ctggcgttga gcgcgctcag tctgagcctg aaaaatgaaa agcgcgtgca tctggcacaa
    13981 ccgtgggctt ccggccctcg ttttttccca ctgccgcagg ggcaaatcgc cgtcactctg
    14041 cgtgacgcac aggcctgctt taacctgaat gccctcgctc agcccacaac ggcgtcgcgt
    14101 ccgctcgcgg tacaacaact gattgccctg atcacgcgcc tggatgtgcc tgcttatcgg
    14161 gccgaactga tagccgaaag cctgtgggag tttattgatg aagaccgcag cgtgcagacg
    14221 cgtctgggcc gtgaagacag cgaatatctc gcccgctcgg tgcctttcta cgccgccaat
    14281 caaccgctgg ccgatatcag cgagatgcgc gtggtgcagg gaatggacgc cgggctttat
    14341 caaaaactga aaccgctggt ctgtgcgctg ccgatgaccc gccagcaaat caacatcaat
    14401 actttagacg tcacgcaaag tgtgattctt gaggcgctgt ttgacccgtg gttaagccct
    14461 gttcaggcgc gggcgttatt acaacaacgt ccggcgaagg gctgggaaga tgtcgatcag
    14521 tttcttgcac agccgctact tgctgacgtc gatgagcgta ctaaaaaaca gctaaaaacc
    14581 gtcctgagcg tggacagcaa ttacttctgg ctgcgttcag atatcaccgt gaatgagatt
    14641 gaactgacga tgaactcgtt aattgtccgc atgggcccac aacacttttc ggttctctgg
    14701 catcagacag gagaaagtga gtgagttcca tccttgagat ttttttcccg ctttgcgccg
    14761 ctgatcccat ccattggcag cgccgtacac ccgatgtgga gcacggtatc tggtctgacg
    14821 ttgctaacga acagctccag caatggctgc aaactgatgc gattcgactc tacattcccg
    14881 gcgaatggat cagcgtctgg caggttgaac tgcctgttgt cgcccgcaag cagataccga
    14941 ctattctgcc cgccttactg gaagaagagc tgaaccagga tatcgatgaa ctgcatttcg
    15001 ccccgttgaa aatcgaccag caactggcaa ccgtagcagt gattcaccaa cagcatatgc
    15061 gcaacattgc gcagtggttg caggaaaacg gcatcacccg cgctaccgtc gcgccggact
    15121 ggatgtccat tccttgtggg tttatggctg gcgatgcgca acgggttatc tgccgcatcg
    15181 atgaatgccg gggatggagc gccgggctgg cgctggctcc ggtcatgttc cgcgcccagc
    15241 tcaatgagca ggatttaccg ctttcgctaa ccgtagtcgg cattgcaccg gaaaagctat
    15301 cagcatgggc tggcgcagac gctgaacgcc tgaccgttac agctctgccc gccgttacca
    15361 cttatggcga accggaaggg aacctgctga cagggccgtg gcagcctcgc gtcagctacc
    15421 gaaaacagtg ggcgcgctgg cgggtgatga ttctgccgat attgctgatt ctggttgcgc
    15481 tggcagtgga gcggggcgtg acgttatgga gcgtcagcga acaggtggcg caaagccgca
    15541 cccaggcgga ggaacagttc ttaacgttgt tcccggagca gaagcggatt gtgaatttac
    15601 gctctcaggt gacgatggcg ctgaaaaaat atcgcccaca ggccgacgat acccggctgc
    15661 tcgccgagtt gtcagcgata gccagcaccc tgaaatcagc gtcactttcc gacatcgaaa
    15721 tgcgtggttt cacctttgat caaaaacgcc agacgcttca cctccagttg cgggccgcga
    15781 actttgccag cttcgacaaa ctgcgtagcg cactggcggc agattttgtt gtgcagcagg
    15841 acgcgttaca gaaagagggt gatgcggttt ccggcggcgt aacgttgcgg aggaaataac
    15901 atgttacgcg ataaatttat tcactatttt cagcaatggc gtgaacgcca gttaagccgt
    15961 ggcgaacact ggctggcaca acacctggcg gggcgttctc cgcgtgaaaa gggcatgtta
    16021 ctggcagcgg tggtgttcct gtttagcgtc ggatattacg tcctcatctg gcagcctttg
    16081 agcgaacgga ttgagcaaca ggagacaata ttgcagcagc tggtggcgat gaacacccga
    16141 ctgaagagcg ccgcgccgga tattattgcg gcgcgaaaat ccgccacaac aacgcctgcg
    16201 caggtatcgc gggttatcag tgacagtgct tccgcgcact cggtagtcat caggcggata
    16261 gccgagcgtg gggagaatat tcaggtctgg atagaacctg tggtgtttaa tgacctgctg
    16321 aaatggttaa acgcactgga tgaaaaatat gcgctgcggg tgacacaaat tgatgtcagt
    16381 gctgctgaga agcctgggat ggtgaatgtg cagcggctgg agtttggacg ggggtaaagt
    16441 atcaggagat taatatcaat aaaattactc tcgttaacgt ggcgagagta attcccccag
    16501 actttataag ctaatttttt tcgtgaaggt tattgtcaga tccaaaagca aaaacccgcc
    16561 ttgtgggcgg gttcttaaga attagtggtg ccggactcgg aatcgaacca aggacacggg
    16621 gattttcaat cccctgctct accgactgag ctatccgggc aacggggcgc attaaacctg
    16681 attcgcctcg tctcgtcaat caaatttctt caatttactg cagactgcac aatctatcat
    16741 cactttgccg ctattgcacg cattgtcagg caaaccaggc ggtccaggcg gcggaaagcg
    16801 gcattgccag aaggagtgct ggaagcatgt tgaccaccgg gaacatttta atgccacaga
    16861 tgcgtaatcc ggttgccagc agcaataaac cgcctacggc gctgaagtct gccatcatcg
    16921 acggtgtggt cagcggtaat atcagcgcgg cagcccacgc cagcgttaac tggatgatca
    16981 gtaatgggat actaattacc gacaccgcaa tacccagcga gcaggcgaag atcatcgccg
    17041 taaagaaatc aagaaatgac ttggcgatta aaatactcgg atcgccggtc atcccttcgt
    17101 tcatcgcccc gaagatcccg gtgccgctgg cgcaaaacag gacaataatc gcgacataat
    17161 tctgaataaa agattcatgc gctggcttct tacgtgagtg acgaaacaga ttttgtgctt
    17221 tggtgactgc tgtattgaca cctttctcca gcaaacaaat ttcgccaatt agagcgccaa
    17281 gtagcgtagc taaaaccatc gctggaaggt tggcacattt caccaccagt aaaataccaa
    17341 tccccagcga tgccagacca aaaatagatg tcatggagac gcggatacgt tccggtaagc
    17401 gttggctgag cagtgcgccg agaacgccac ccagtaatac tgcactagcg ttgataaaag
    17461 ggccgatgac cacaagagtt cctgttaact gtattgtttg attttcttat tatgaccgtt
    17521 tggtgccgtc gatggcttgt ttgttcgcaa agtcctggct tgcacgcttt agcgaaaggt
    17581 gccatgattg cgcgaatttt ctcctctctg tacggagttt gcccgatgca cgccacctcc
    17641 ttacattctc tcgcttatcg ccgtttcgcg cgaaacgttt ccctttctat gttactgctc
    17701 atgcggtgaa gttaacgcac gctcactgca ggacaacagt aaaatcagag cgtttctgct
    17761 tttactgatg tctggcggtc ggagctggtg accagtttga cccatatctc atggggcagg
    17821 gttttccacc ttgtccggta ttcttacttc cccgaaacgg gtttgcgctt atgaaatcaa
    17881 tgaatattgc cgccagtagt gatctggtat cccgactttc ttctcatcgt cgcgtggtgg
    17941 cgttgggaga tactgatttt acggacgtcg cggcagtcgt cattaccgct gcggatagtc
    18001 gcagtggcat tcttgcgttg cttaagcgca ccggttttca tctaccggtg tttttgtatt
    18061 ccgaacatgc tgttgaatta cctgcgggcg ttacggcggt aatcaacggc aacgagcagc
    18121 aatggctgga gctggaatcc gcagcctgtc agtatgaaga gaatttgctg ccaccgtttt
    18181 atgacacgct gacgcagtac gttgagatgg gcaacagcac ctttgcttgc cctggacatc
    18241 aacatggtgc gttttttaaa aagcatcctg ccggacgcca tttttacgat ttctttggtg
    18301 agaacgtctt tcgcgccgat atgtgtaacg ctgacgtaaa attgggcgat ctgcttattc
    18361 atgaaggatc ggcgaaagat gcgcagaaat tcgcagccaa agtctttcat gccgataaaa
    18421 cctattttgt gctgaacggc acatcggcag cgaataaagt ggtgacgaat gcgctgttaa
    18481 cgcgtggcga tctggtgctc ttcgaccgta acaaccataa gtcgaatcat cacggcgcgc
    18541 tgattcaggc gggggcgacg ccggtctatc tggaagcttc acgcaacccg tttggtttca
    18601 ttggcggtat tgatgcgcac tgttttaatg aagagtatct gcgccagcaa attcgcgacg
    18661 ttgcgccaga aaaagctgag ttgccacgcc cgtttcgcct ggcgattatt cagctgggaa
    18721 cctatgacgg cactgtctat aacgcccgtc aggtgatcga taccgttggg catctgtgtg
    18781 attacattct gtttgattcc gcgtgggtcg gttatgaaca atttatcccg atgatggcgg
    18841 atagctcgcc gctgctgtta gaacttaacg aaaacgatcc ggggatcttt gtgactcagt
    18901 cggtgcacaa acagcaggcg ggattctcac agacgtcgca gatccataaa aaagataacc
    18961 atatccgcgg acaggcgcgt ttttgcccgc ataagcggtt gaataatgcc tttatgctcc
    19021 atgcttctac cagcccgttc tacccgctgt ttgccgcgct ggacgttaac gctaaaattc
    19081 atgaaggaga gagtgggcgt cggctgtggg ctgagtgcgt tgagttgggt attgagtcgc
    19141 gcaaggctat tcttgcgcgc tgtaagctgt tccgcccgtt tatcccgccc gttgttgatg
    19201 gcaaattgtg gcaggattat ccgacatctg tgttagccag cgaccgccgt tttttcagtt
    19261 ttgagccggg ggcgaagtgg cacggctttg aaggatatgc cgcggatcag tattttgttg
    19321 atccgtgcaa gctgttactc actacaccag gtatcaatgc cgaaactggc gaatatagcg
    19381 actttggcgt tccggcgacg attctggcgc actatctgcg tgagaacggc attgtgccgg
    19441 agaagtgcga tctcaactcc attctgttct tattaactcc ggcggaaagc cacgagaagc
    19501 tggcacaact ggtggcgatg ctggcgcaat ttgaacagca tattgaggat gactcgccgc
    19561 tggctgaggt gttgccgagc gtttataaca aatatccggt gcgctatcgc gactacacca
    19621 tgcgccagtt gtgtcaggag atgcacgatc tgtatgtcag tttcgacgtc aaagacctac
    19681 aaaaagcgat gttccgccag cagagtttcc cgtcagtggt gatgaacccc caggatgcgc
    19741 atagcgctta tattcgcggt gaagtggagt tggtgcggat tcgtgatgcc gaagggcgaa
    19801 ttgcggcaga aggggcgttg ccttatccac ctggcgtgct ttgcgtggta cccggggaag
    19861 tctggggtgg ggcggttcaa cgttatttcc ttgcactgga agaaggggtg aatttgttgc
    19921 cgggattttc gccggagctg caaggtgttt atagcgaaac cgatgcggat ggcatgaaac
    19981 ggttgtacgg ttatgtgttg aagtaagaat aaaaaaaacg ggtcaccttc tggcgacccg
    20041 tttttctttg cgtaattagt ggctaaccgt ctgtgtgcct gtcgggacac gaacgtgttt
    20101 atatttgaac atcgccatga acgcgaaggc cagaaccacg gagtaaccag cgaaaatcaa
    20161 ccatacggtc tgccagtcgg taatgccgtt ttgggtgtac atctcaacaa ctttaccgct
    20221 cacgatgccg ccgaggatac agccgaagcc gttagtcatc atcaggaaca tcccttgtgc
    20281 actggcgcga attgccgggc taacttcttt ttcgacaaac accgaaccag agatgttgaa
    20341 gaagtcgaat gcgcaaccgt aaacgatcat cgacagtacc agcagtacag taccgaacgg
    20401 agtcgggtcg ccgtaagcaa acagcgcaaa acgcaggatc cacgccacaa tactgatcat
    20461 cattacgttc ttaataccgt agcggcttaa gaagaacggg atggtcagaa tgaacagggt
    20521 ttcagagatc tgcgaaatcg acatgatgat tgacgcatgc tgcacaataa agctgctggc
    20581 aaacatcgga tctttgtcga agctgtgcag gaaggtatta ccgaacatgt tggtaatctg
    20641 cagttccgcg cccagcagca ttgagaagat aaagaagatt gccatacgct tgtttttaaa
    20701 cagcgcgaat gcatcgaggc ccagcagggt tgtccagctc tgattcgctt gctgtttagc
    20761 aaccggaata tgcggcagag tcagggtaaa cagaaccaga atggcggaaa gtgctgcgcc
    20821 aatatacagc tgcatgtggc ttaattcgaa gccagacagg ctcaccaccc acattgccat
    20881 gataaagccg atggtgcccc agatacggat tggcgggaag tcagtaacga tatccatccc
    20941 ggcattttgc aggcgatagt aagagatggt gttgattaac ccaagcgttg gcatataagc
    21001 aaacgagtta atcaatatca caaggaacat cgcctccggt gtcgtgacct gtgccgccat
    21061 gaacagcgtg atagcgccaa tggtgtggca aatggcatat acccatttcg cacttaacca
    21121 tttgtcggcc acaatcccca gcagcgcagg cataaatacc gcggcgatac ccagtgagct
    21181 ataaactgcg ccaatagaag caccgtcaaa cttcagggta acaaacatat aggagccgag
    21241 ggtcgtcagc caacttcccc acagacagaa ctgcagaaaa gagaggattt tcagctgcag
    21301 cttaagattc atgttaattt cctcacatcg tgatgcggat gagtgtttga aaaagcacat
    21361 ttgaccgtgc gagatgatgg gattctatca acttcgcgga ctggtttttt gttacctgtg
    21421 gcaaataatt gcaaacattc ccaatttgca aaacgaaatt gtgatgtgga taacattttt
    21481 gcccctgagc atcgtcaggg gcggttaatg gaattatcgg cggcggtagg atttttgcgc
    21541 ggtattcact ttataaagat aacggcgaga ttccgcagag ggatggcggg tcgtcagcgt
    21601 ctgataaaca tcgcccggcg tcatggtgtt aataatattg gcagcctgaa tcttatcatt
    21661 cgaaaagact cgcagcacgc tgcctgcgcc gccgttatag gcggtgatga cggcataacg
    21721 ccgcgatgtt gggttatcaa ttccgccgag ataaacattg ttcagcatcg ccagatacgc
    21781 ggtgccggta tcaatattgc tggcaggatc aaacaagaaa ctgcggctcg gcgtgccgga
    21841 tttcccctgc gagcggaaca catctttccc ggcagtatgt tgtaccacct gcattaatcc
    21901 cagcgcatcg gaacggctga ccgcatatgg gttaaaggaa gattcggtct gcataatcgc
    21961 cagaatcagc gactcatcaa cgccatattt ccgtgacgcc tggcggacca tgccgagata
    22021 tttgtgcgca cgtttatcaa ggtggttcgg caccatgtta atggtaacgc tgtagatgat
    22081 acgcagcccg ttgctgcggc tcttcagacg gtttttcagc agataatccg cgaagttgct
    22141 cgcgcgacct tcccagcgaa tcggctgccc ggtgttgtcc accacctgac cgtaaaggaa
    22201 aggttctttc gaaatcgtaa tatcatcaac gtcggaatag agatcgaccg aactcggatc
    22261 gtcacccatc agcaacgttt tgataattgc ccggcgcaaa tgcgcggcag gttctgtccc
    22321 ggcgatagtt tcgatagtaa tcgtaccgtc atcgaagttg atgtggctgc gggtctgata
    22381 ttgatcggtg tatttcacgt agtccttagg accggcgatc accacctctt tgaatcccca
    22441 gatgttctca atattgtggg caaattgccc catcagaata tcaaaaccgt tggtatcttt
    22501 gacccaggct tcgttatagg tatcgccttt tttggtcgtc gaacaggaga tgagcaacgg
    22561 cgcaatcaaa gccagcgcga gatatttttt catcattccg ggtgcgtgtt gtgtttgttt
    22621 ttatgcaagt tgccggaggc gtgctccggc actgttttta ttttttatct tccggcgtat
    22681 agccctcgat atgcacctct ttaccctcga acaggaagtt gaccatctcc tgctccagca
    22741 gcttgcggtg ctcggcattc atcatgttga gtttcttttc attaatcagc atggtttgtt
    22801 tgtgctgcca ctgcgcccag gcttctttgg agatctcgtt atagatgcgt tttcccagct
    22861 cgccggggta cagctgaaaa tcctgacctt ctgcttcacg ttgcaggaaa gtacaaaaaa
    22921 tcgttctgct cataaatcat cctctttatc gactcacgcg ctaaaccggc gcgccagtgc
    22981 gtaactgctg taacaaacgc tccacgggag ccgccaggcc aactgacggc ggttgcgcta
    23041 agttatacca gagcgcattg ccttcatcca tgcagccggt gaatgacgac acgggaagcc
    23101 acataggcac aatatctaag tggaaatggc tgaaggtatg ccgaaacgcg gtcagctgcg
    23161 tcaggttatc ggcagcaatc tgccgttgcg ccagccactg ccgcaaactt tcttcgtcgg
    23221 caaactgcgg gaaacagtat aaaccgcccc acaatccgct cggcggacgc tgcgccagca
    23281 atacttcatc ttcgtgctgt aacaggagaa agtagccggt gcgctccggc agcgtctgtt
    23341 tcggtttttt gcccggataa agcgaccagc tattgttggc ggcggcaata catccgtttt
    23401 gtagcggaca gagcgaacat ttcggtttcg agcgcgtaca aatcatcgca cccaaatcca
    23461 tcatcgcctg attaaaccgt tccacgccaa ccgcgggcgt cacctgctcg ctcaaactcc
    23521 ataatttatt ctcgacctct tttttccctg gccagccgct tacagcatag cagcgcgcca
    23581 gcacccgttt gacgttaccg tcgagaatcg gaaagtgctt acccagagaa agcgagagaa
    23641 tcgcacctgc ggtggaacgc ccgacgcccg gtaacgccgc gacttcttca aaggtttccg
    23701 ggaatttacc gccgtgtaag gtcgccactt gttgtgccgc tttatgcagg ttgcgcgcgc
    23761 gggcgtaata gccaagcccg gtccacaagt ggagaacttc gtcgagcggc gcattggcga
    23821 gatcggtcac cgtcgggaag cgcgccataa agcgttcaaa ataggggata acggtcgcaa
    23881 cctgagtttg ttgcaacatc acttctgaga gccatacttt gtagggcgtc ttgtcaattt
    23941 gccagggcag cgttttccgc ccgtatttat cgtaccagtc cagaacctgg gctgaaaatt
    24001 gcgacgcttg catggtcacc gaattcactg ttgttggggg caagattgca gcacagcgac
    24061 ggcagggtgt aaaccggaac tttccgcagc cacggcctta tcatgcttgc atccggcaac
    24121 taactttgga taatgcccgt tttcagaaca ctttcacaag cgactaaatc tttatgaaaa
    24181 acgacgtcat ttcaccggaa tttgatgaaa acggccgccc actgcgccgt atccgtagtt
    24241 ttgtgcgccg ccaggggcga ctgaccaaag gccaggaaca tgcgctggaa aactactggc
    24301 cggtgatggg cgttgagttc agcgaagata tgctggattt ccccgcgctt tttggccgtg
    24361 aagcgccggt gacgcttgag attggttttg gcatgggggc atcgctggtg gcaatggcta
    24421 aagatcgccc cgagcaggac ttcctcggca ttgaagtgca ttcaccgggc gttggtgcgt
    24481 gcctggcttc tgcgcatgaa gaaggtttaa gcaacctgcg agtgatgtgt cacgatgcgg
    24541 ttgaagtgct gcataaaatg attcctgaca attcattgcg catggtgcag ctctttttcc
    24601 ctgacccgtg gcacaaagcg cgccataata aacgccgtat cgtacaggtg ccgtttgccg
    24661 aactggtaaa aagcaaactg cagctggggg gggtattcca tatggcgacc gactgggaac
    24721 cttatgcgga acatatgctt gaagtgatgt cttctattga cggttataaa aacctgtcag
    24781 agagcaatga ttacgtaccg cgtccggcat cacgtccggt gacgaaattt gaacaacgtg
    24841 gtcatcgtct tggtcacgga gtatgggact taatgttcga gagggtgaaa taatggcaaa
    24901 gaaccgtagc cgtcgtctgc gtaaaaaaat gcacatcgac gaattccagg aattaggatt
    24961 ttcggtggca tggcgattcc cggaaggtac atcggaagaa cagattgata aaaccgttga
    25021 tgattttatt aacgaggtta tcgaaccgaa caaactggcc tttgacggca gcggttatct
    25081 ggcctgggaa ggtctgatct gcatgcagga aatcggcaaa tgcaccgaag aacatcaggc
    25141 gattgtgcgt aagtggctgg aagagcgcaa actggatgag gtacgcacca gcgaactttt
    25201 cgacgtttgg tgggactaag aaagcatacg ggcgatgaca aatgcaaaac tgcctgatgc
    25261 gctacgctta tcaggcctgg aaagatgcac gatcgagtag gcgggataag gtgtttacgc
    25321 cgcatccggc atggaaaacg cgtactttgt tatcaatctg gggccagcaa atgctggcct
    25381 gattttttct tgagggaaga ctatgatgcg caaaatgctg ctggcggcag cactttcagt
    25441 gacggcaatg accgctcacg ccgactacca gtgcagcgtc acgccgcgtg acgatgtgat
    25501 tgtcagcccg caaaccgtgc aggtgaaggg cgaaaacggc aatctggtga tcacgccaga
    25561 cggcaacgtg atgtataacg gtaagcaata ttccctgaat gccgcccagc gcgagcaggc
    25621 gaaggattat caggctgaac tacgcagcac gctgccgtgg attgatgaag gcgcgaaaag
    25681 ccgcgtcgaa aaagcccgta ttgcgctgga taaaattatc gttcaggaga tgggcgaaag
    25741 cagcaaaatg cgcagccgtc tgaccaaact tgatgcgcag ctgaaagagc agatgaaccg
    25801 cattatcgaa acgcgcagcg atggcctgac gtttcactat aaagccattg atcaggttcg
    25861 cgccgaaggc cagcaattag tgaatcaggc aatgggtgga attttacagg acagcattaa
    25921 tgaaatgggc gcgaaagcgg tgctgaaaag cggcggtaac ccattacaga atgtgctggg
    25981 aagcctgggg gggctgcaat cctcaatcca aaccgagtgg aaaaagcagg aaaaagattt
    26041 ccagcagttt ggcaaagatg tttgtagccg cgttgtgact ctggaagata gccgcaaagc
    26101 cctggtcggg aatttaaaat aatcctctat tttaagacgg cataatactt ttttatgccg
    26161 tttaattctt cgttttgtta cctgcctcta actttgtaga tctccaaaat atattcacgt
    26221 tgtaaattgt ttaacgtcaa atttcccata cagagctaag ggataatgcg tagcgttcac
    26281 gtaactggag gaatgaaatg gagtttttca aaaagacggc acttgccgca ctggttatgg
    26341 gttttagtgg tgcagcattg gcattaccca atatcaccat tttagcaacc ggcgggacca
    26401 ttgccggtgg tggtgactcc gcaaccaaat ctaactacac agcgggtaaa gttggcgtag
    26461 aaaatctggt taatgcggtg ccgcaactga aggacattgc gaacgttaaa ggcgagcagg
    26521 tagtgaatat cggctcccag gacatgaacg ataatgtctg gctgacactg gcgaaaaaaa
    26581 ttaacaccga ctgcgataag accgacggct tcgtcattac ccacggtacc gacacgatgg
    26641 aagaaactgc ttacttcctc gacctgacgg tgaaatgcga caaaccggtg gtgatggtcg
    26701 gcgcaatgcg tccgtccacg tctatgagcg cagacggtcc attcaacctg tataacgcgg
    26761 tagtgaccgc agctgataaa gcctccgcca accgtggcgt gctggtagtg atgaatgaca
    26821 ccgtgcttga tggccgtgac gtcaccaaaa ccaacaccac cgacgtagcg accttcaagt
    26881 ctgttaacta cggtcctctg ggttacattc acaacggtaa gattgactac cagcgtaccc
    26941 cggcacgtaa gcataccagc gacacgccat tcgatgtctc taagctgaat gaactgccga
    27001 aagtcggcat tgtttataac tacgctaacg catccgatct tccggctaaa gcactggtag
    27061 atgcgggcta tgatggcatc gttagcgctg gtgtgggtaa cggcaacctg tataaatctg
    27121 tgttcgacac gctggcgacc gccgcgaaaa acggtactgc agtcgtgcgt tcttcccgcg
    27181 taccgacggg cgctaccact caggatgccg aagtggatga tgcgaaatac ggcttcgtcg
    27241 cctctggcac gctgaacccg caaaaagcgc gcgttctgct gcaactggct ctgacgcaaa
    27301 ccaaagatcc gcagcagatc cagcagatct tcaatcagta ctaatcacct cgccccggta
    27361 tcgtgccggg gctttttcac ttcagactta cgtccattgc caattttcat taccctaatg
    27421 ataatcaccg gaataaatta ttccgcgtga ggtttttcgg gtgaaaaagc aatggattgt
    27481 tggcacggca ctgcttatgt tgatgactgg taatgcctgg gcagatggcg aaccgccaac
    27541 tgaaaatatc ttaaaagatc aattcaaaaa gcagtatcac ggcattctca agcttgatgc
    27601 tatcacctta aaaaatcttg atgctaaggg taatcaggcc acctggtcag cggaaggcga
    27661 tgtctcttcc agtgacgatc tctatacctg ggtcggtcag ttggcagatt acgaactgct
    27721 cgaacagacc tggacgaaag ataaaccggt aaaattctcg gcgatgttaa ccagtaaagg
    27781 aacgcccgcg tctggctggt cggtgaactt ttactctttt caggcggcag ccagcgatcg
    27841 tgggcgggtg gtcgacgata tcaaaacgaa taataaatat ctgatcgtga atagcgaaga
    27901 tttcaattat cgctttagtc agcttgaatc tgcgttgaat acccagaaga actcgattcc
    27961 tgcgctggaa aaggaagtga aagcgcttga taagcaaatg gtggcggcgc agaaagcagc
    28021 ggatgcatat tggggaaaag atgcaaacgg taagcaaatg acccgcgaag aagcctttaa
    28081 aaaaattcac caacagcgtg atgagtttaa taaacagaac gatagcgagg cgttcgcggt
    28141 taaatatgac aaagaggttt atcaaccggc gattgcggca tgtcataaac agagtgaaga
    28201 gtgttatgaa gtgccgattc agcagaaacg agatttcgat atcaacgaac aacggcgaca
    28261 gacttttctg caatcacaaa aactcagccg taaattgcag gatgactggg taacgctcga
    28321 aaaagggcaa tatccgttaa cgatgaaagt ttcagaaata aacagtaaaa aagtcgcgat
    28381 tttgatgaaa attgacgata tcaaccaagc taatgaacgc tggaaaaaag ataccgaaca
    28441 actgcggcgc aatggggtga ttaagtaagt ttgatatagt gcacgatttg tagcaggatg
    28501 cggcgttgac cccgcatccg gcaatcaacg tctgatgcga cgcttgcgcg tcttatcaga
    28561 cctacaaccg ctgccacatg tcggctggat aaggcgttca cgccgcatcc ggcaatacaa
    28621 gtttactcag ccagaaaaag ctccagcagc gaatttaaaa acagcttccc atgttccgtt
    28681 atctgccagt aatccgcaca ttcggtgaga taaccctggg caatcgcctc gtctaactgt
    28741 gggcgaatca catcttcgca aagcccggta tacgcactaa actctgcgcg tggcgcggcc
    28801 tccagcaagc gaaagcggtt cataaagaac tcaaacggct tatctgcggc ttcgacatca
    28861 cgctggcttt ccagatacct tccttgcata aaaccacgcg gatgacgcgt tttagtggta
    28921 cgcagaatgc gcccatccgg gaaggtcact ttgccgtgcg cgccgcagcc aataccgatg
    28981 tagtcgccaa agcgccagta gttgagattg tgctggcact gataactggg tttggcgtaa
    29041 gcggaagttt catattgctg ataacccgct gcggttaata actgatgccc ctgttcgaat
    29101 atatcccaca gcgcgtcgtc gtccggcagt accggcgggc gtgaaccaaa cagcgtatta
    29161 ggttcgatgg tcagttgata ccaggaaaga tgcggcggat tcagttcaat ggcctggcgt
    29221 agatcgccaa gcgcctcttc cagtgattga tccggtagtc catgcatcaa atcgaggtta
    29281 aagctacgca gccccagccc gctcgccagc tttgccgcgc gtttcgcttc ttgcgggcca
    29341 tgaatacgcc caagtcgttt cagcttttct tcgctaaaac tctgcacacc aatagagatg
    29401 cggttcacac cagcacgctg ataatcgaca aagcgatcgg cttctaccgt accagggttc
    29461 gcttccatag taatttctgc atccgctgcc agcggcaaac gcgcacgcac gccgtccagc
    29521 agcgtttgca tcgccgggcc ggaaagcagg ctcggcgtac caccgccaat aaagattgtc
    29581 tttacttcac ggccctgagc gtaagccaca tcattgtcca gatcgcaaag cagatgctga
    29641 acgtaatcgt cgtgcggcac ttctcctttc aacgcgtgag agttgaaatc gcagtacggg
    29701 catttctgca cgcaccacgg gatgtgaatg tagagactca gcggcggtaa tttaaccatt
    29761 acgtaaagcg tccaacagca gtttcaatgc ctgaccacgg tgggaaatgg cgctcttttc
    29821 ttcgcgggtc agttcggcag cggttttccc ttcggaaggt acgaagaaga ttggatcata
    29881 accaaagcca ccagtgcccg ctggttcacg agtaatcacg cccggccagc taccgtggca
    29941 caccagcgga gtcggatctt ccgcgtgacg cagatatacc agcacgcagt ggaaacgcgc
    30001 ctgacgttgg tcgtccggta cgtctttcag tgtttccagt agtttttgca gattctttag
    30061 atcggtcgcg tcttcaccgg aataacgcgc ggagtaaatg cccggcgcgc cgccaagcac
    30121 atctaccgcc agaccagagt cgtcggcaat tgccggtaaa ccggtcactt ttgccgcatg
    30181 gcgcgctttc agaatcgcgt tttcgataaa ggtcaggccg gtttcttcag cggaatctac
    30241 gccgaggtct gtttgggcca cgatatcaag accgaagtcg ctaagcagcg acgccagctc
    30301 acgcacttta ccggcattgc cggttgcgag gacaactttt tgcataggat acctaattaa
    30361 ttaacgccgc gatttctggc gggatttgtt gcggattaat gattttaatt tgtttgtggc
    30421 ggccaagttc gcctttttca atcaccacca ggcttttggc aacccggaat tgcttaccga
    30481 gaaacttcac cagatgactg ttggcctggc cgtcaaccgg cggcgcggta atggcgactt
    30541 taacttcgtc gccatgtaaa ccgacaatag aatcacggct ggctttcggc tgaatataga
    30601 gccgtaaaac cagaccgtca tcattaactg ttacggcact cataacgcca tccacagccc
    30661 cggcagcagc atgtttccgg ttgcctgtaa tacttctgcg acacccatat tgatgacata
    30721 cagcagcaga acgaggatca tcggcgagaa atcaatccca cccattgccg gtagcaggcg
    30781 gcgaatcggg cgcagcagcg gatcggccag ctgaatcagc acgtattcaa tcggactacg
    30841 accctggctt acccagctca taatcgccat caccagcagg acccagaaaa tcagcaagcc
    30901 gatggttttc agcagaatca gtaaaccggc aatccagatg attggcagga aggtcaccac
    30961 tttaaacagc acaatggctt tgataaaact gagaatatag gcaaccagca gcgaggcgct
    31021 gtcaatggga cccattgccg gaataacgcg gcgcagtggc ccgataattg gctgcgttac
    31081 cttcactaca aactgtgaga aggggttgta aaaatcacaa tgagcccact gcatccagat
    31141 gcgtaataac agcaccatgg tatacagctc aatgaccgtt gaaagcagga aagtcaacgt
    31201 attcatggcg ttcctttaat tccttatttt ttagagtaat cacgcgcacc aaaaattgca
    31261 gtgccgatac gaaccatcgt gctacctgcc gcaatggcgg cttccatatc gtccgacatt
    31321 cccagagaga gcgtgtcgat atgcgggtag cgcgttttca gtccggcaaa tgctacagcc
    31381 atttggcgtg caacttcaaa ctgccttaca tattctgact caggcgcagg gattgccatc
    31441 agcccgcgca gacgtaaacg cggtagttca gcgaccgccg ctgccagctc gtccagttct
    31501 gccagttgaa tcccggactt actgttttca tcactaatat taatttgaat cagtacgtta
    31561 agagggggaa gttctgccgg gcgctgatcg ttgaggcggg tagcaatgcg caaacggtcg
    31621 atggtatgac accagtcgaa atgctctgcc accaggcggc ttttattaga ctgcaacggg
    31681 ccaataaaat gccattctaa tcctgttacg cccagttcct gaaagtggcg aattttatct
    31741 accccttcct gaacgtagtt ttcaccaaat tgacgctgcc cggcatcaat ggcttctgcg
    31801 atggcgctcg caggttttgt tttactgact gcaagcagcg taatttcttc tggagaacgg
    31861 ccgcaacgcg ttgcagccgc tgagattttg tcccggacct gtgccaggtt atgcgcaata
    31921 tcgttcattt tccgaggatc ttagtatgaa tatggaagaa attgtggccc ttagtgtaaa
    31981 gcataacgtc tcggatctac acctgtgcag cgcctggccc gcacgatggc gcattcgtgg
    32041 cagaatggaa gctgcgccgt ttgatgcgcc ggacgtcgaa gagctactgc gggagtggct
    32101 ggatgacgat cagcgggcaa tattgctgga aaatggccag ctggattttg ccgtgtcgct
    32161 ggcggaaaac cagcggttgc gtggcagtgc gttcgcgcaa cggcaaggta tttctctggc
    32221 attacggttg ttaccttcgc actgtccaca gctcgaacag cttggtgcgc caccggtatt
    32281 gccggaatta ctcaagagcg agaatggcct gattctggtg acgggggcga cggggagcgg
    32341 caaatctacc acgctggcgg cgatggttgg ctatcttaat caacatgccg atgcgcatat
    32401 tctgacgctg gaagatcctg tggaatatct ctatgccagc cagcgatgtt tgatccagca
    32461 gcgggaaatt ggtttgcact gtatgacttt cgcatcgggg ttgcgggctg cattgcggga
    32521 agatcctgat gtgattttgc tcggagagct acgtgacagt gagacaatcc gtctggcgct
    32581 aacggcggca gaaaccgggc atctggtgct ggcaacctta catacgcgtg gtgccgcgca
    32641 ggcagttgag cgactggtgg attcatttcc ggcgcaggaa aaagatcccg tacgtaatca
    32701 actggcaggt agtttacggg ccgtgttgtc acaaaagctg gaagtggata aacaggaagg
    32761 atgcgtggcg ctgtttgaat tattgattaa cactcccgcg gtggggaatt tgattcgcga
    32821 agggaaaacc caccagttac cgcatgttat tcaaaccggg cagcaggtgg ggatgataac
    32881 gtttcagcag agttttcagc agcgagtggg ggaagggcgt ttgtgacgtt gccatgcccg
    32941 atgtgcaagc ttatcgggcc tacaaaggca ataaacgtct gatgcgacgc ttgcgcgtct
    33001 tatcagacct acaaccgctg ccacatgtag gccggataag gcgtaaggcg cgatgatatt
    33061 atacactcca gcaggcgttt aaatcgcttt aatatccctg ctcgaaatag ctttcgagaa
    33121 taataaccgc agaggcagag tcgactttgc ctttatttag cgcccgatag ccgccctgtt
    33181 caaacagacc ggaacgggct tccacggtgc taagacgttc gtcatggagc tttacttcaa
    33241 caccgaaacg accatgaata cggttggcaa atttacgcgc tctggcagtc aatggttgct
    33301 cggtgccgtc catattcagc ggcaaaccga cgatgatttc gtccggctgc cactctttca
    33361 gtaaacgctc gataatgttc cagtccggcg taccgtcctg tgctttaatt gcaggcaaag
    33421 ggcgagcggt gccggtaatg cgttggccga ccgctacacc aatgcttttg gtaccgaagt
    33481 cgaaggcgag taaggttcca ctcatcaggc gtgccctgcc acaccaggca tggtgagaat
    33541 atccacacca atcagttttg ccgcctcgcg ccagcggtcg gcaatcggtg ttttgaacag
    33601 aatattcaga tccgccgggg ccgttagcca cgcgttatcg agaatttctt gttccagttg
    33661 acctttttcc caggaggcat aacccagagc caccaatacg tcagacggtt gtttatcggt
    33721 gccgagcgtt tccagcacat cgcgggaagt ggtcatcact gtgttgtctg aaatgcgaat
    33781 gctggaagca aaattggagg gcggagtatg caaaataaac ccgcgatctt cagccagcgg
    33841 accgccgagc ataaccggtt tatccagacg gattgattca tcacgcggct ccggcgtgat
    33901 cttcagcttt tccagaatcc cttcaatttt gagattttcc agcggcttgt tgacgatgat
    33961 ccccattgca ccattggtat tatgttcgca aatgtagacc acggaacgac ggaaaatcgg
    34021 atcctggaga gcaggcatgg caataagaaa gtgatgctgt aaattcattg tcagaggttc
    34081 tgtttcctgg ttcaaaaaag caacagcgcc cagtatgcgt ggaaagaagg gcgctgtcac
    34141 tcagagtctc aacgagatcc ttctcgctaa ggtgggttac tgctgctgta aacgtgcttc
    34201 gatggcatcc attaacattc cggtgatcga caccggaaac tctgcttcaa tctcacgaat
    34261 acaggttggg ctggtgacgt taatttcagt cagacggtcg ccgatgatat ccagaccaac
    34321 aaaaatcagc cctttttctt tcagcgtcgg cccgatctga cgggcgattt tccagtcact
    34381 ttccgtcagc ggacgaggtt caccgcgtcc accggcagcc agattgccac gggtttcgcc
    34441 cccctgcgga atacgcgcca ggcagtacgg taccggctcg ccatccacca ccagcacgcg
    34501 tttgtcgcca tctttaatgg ctggcaggta attttgcgcc atgcagtagc gagtgccatg
    34561 ctcagtcagg gtttcggcaa tcacgccgag gtttggatcg ccttctttca cgcggaaaat
    34621 cgacgcgccg cccataccgt ccagcggctt aagaatgatg tcgctgtgtt tctcccagaa
    34681 cgcttttagc tgcgctttat tgcgcgtaac cagcgtttct ggcgttaagt cagagaacca
    34741 ggcggtaaac agtttctcgt tacagtcgcg caggctctgc ggcttgttaa cgatcagcgt
    34801 ccctttctct tcggcacgtt ccagaatata ggtcgcgtag ataaactcgg tatcaaacgg
    34861 cgggtcttta cgcatcagga tcacatcgag atcggccagc ggcagatcct gttcaccgac
    34921 gaatgaaaac cactcttcgt agttctgctt cacgttcagc gtgcgggtat gggcgcgggc
    34981 ttcgccattg atcagataca gatcgcccat ctccatatag tgaagttcgt aaccacgacg
    35041 ctgtgcttcc agcaacatag cgaaactgga atctttcttg atgttgatgt ttgcgatggg
    35101 gtccatcacg atgccgagct tgatcattat tcttctccgt tagcccaaat cgccaaatcg
    35161 tacttgtagc gcggtaatgg cggtgagcgc agttgtctct gtacgcaaaa cgcgaggtcc
    35221 caacaggata tcagtaaatt gatagcgggc agtcatggca atttcatctg ccgataaacc
    35281 gccttccggg ccaatcagca ggcggacgcg ttcaaccggt aacggcaacg tattgatgct
    35341 gttactggcg cgcgggtgaa gattcagttt cagtccttca tcctgctctg cacaccaggc
    35401 ttccagatcc atcgctgggc ggatttccgg cacccggtta cgaccacact gctcacaggc
    35461 agcaattgca atcttctgcc actgctggag cttcttgttc agacgttcac tatctagttt
    35521 aacgccgcag cgctcagaaa aaagtggcgt aatgaggctt acaccgagtt cgatcgattt
    35581 ctggatcgta aattccattt tttcaccacg cgacatcacc tgaccgaggt gaatatgcag
    35641 cggagattcg cgatcgtcga tcttgccttc cagcaccttc acttccacgc tttttttgct
    35701 ggcgctggta atttcggcgt caaagacctg gttgctaccg tcaaacaatt gcaacgcctg
    35761 ccccggcccc atgcgcagta cgcgcccgat atggttggcg gcatcttcgc aaagcgcgat
    35821 gtgagaatgg ctggtcagtg gttcaggatg ataaatgcgg gggatacgca tagttaaaaa
    35881 tccgcgtcgt ctccccacgc ggttgtacgc gtggggtagg ggttaacaaa aagagtcccg
    35941 ctagtgtagg ttagctcttt cgcgcctggc aagcgcgttg cacatacggg ttatgattgc
    36001 cttgcacctt cgcgatgcgt tcatcgcgct cgcactccca gtcggtaacc ggatacatct
    36061 tgttccatgc gttgaacagc tgcgtttgct ggcgagagag tgtcaggttg tattggtcgc
    36121 gcatatagaa gtaggtgcgc gcaatggcac cgcgtgcacg cgctggtggt tcggcagctt
    36181 tttctttgaa atcgaccttc atggcgcatt gaccgtactg gccttcgccg ccattccact
    36241 ggctgtacat aaagttgccg cgatcgccat tcacctcacc gactgacggc tgcaggttat
    36301 gcatatcgct ttccatcttg cgatagaccg gatctttagc gcagttttta cgtccaccgt
    36361 cctgccagca ctggcgctgg tgaccgaact gccaggcggg aacgacgtgt tcccactcta
    36421 cgcggctggc tcggttttca tttttgcgca cctgatagcc gcacgattgc agatcaacaa
    36481 cgcctttttt gccctgccag ttaattttac atccgcaata aaacgtaccg ggcgcgtcag
    36541 cgtggacttt taccgccgcg gctttcgcct gagaaaaact attgatacct tcggccaacg
    36601 ccgggccgga aaatgctgcg ctcagtacca ccgcagcaat agacaaataa cggtacatca
    36661 cttactccgt gttaaaacga gccagcaacg tagcgaaagt tgttttggta tgcaatcagc
    36721 cagatcagga aagttcctga tagttcagat ggttatttcg caaccagctg ttcaccgcag
    36781 tgaacacagc gatagacggc ctcgccacga actacgcgat tatggcggcg tacggtaagc
    36841 tgatgctcct ggcacttgca gcggtagggg aaggtgttgc gacgcacgga ttgcagttcg
    36901 aactgatgcg tacgacgggc gggaacgccc agcacgcttt ccatcatcca cttccactct
    36961 ttgccatgtg gcgctacgcg gccgaaatgt ttccatacca gcaaatgtgc cagttcgtgc
    37021 ggtaccactt cttcaataaa agcttcactg ttttccaaca gcaaaacggg attgaggcgg
    37081 atttcatagc tttccagcca ggctgttccg gcggaggttc cgcgctgggt gtaagagagt
    37141 tttggctccg ggtagttacg ccctagcttc aggttggcct gggcgagttt ttcccgcagg
    37201 cggcgcataa cggcctgctg gatggcgata gggagacggg atgttttcat agcggcagag
    37261 gatagagcga agaaaagatg actgcaagag gtggcttcct ccgcgatggg aggaagcctg
    37321 aggagattaa tcgtgagcgc cgatttcgcg cagtttacga cctttcatca gattacgttc
    37381 aatatgttcc agcgaaacgt gtttggtttc cggtaccagc cacaatgtca gcaggataaa
    37441 cagtacgttc agagccgcat acacccagaa ggtgttggcg ttacccagcg tgttgagcat
    37501 ggtcaggaac gttgcgccaa cgatcatgtt ggcaatccag ttggtggcgg tggagcaggt
    37561 gatgccaaaa tcgcggcctt tcagcggctg aatttcggag cacagtaccc aaatcagcgg
    37621 accggcactc atggcaaaac cgacaataaa catcagcagc atggcgatgg cgaaatactg
    37681 cgctgacgga gagtgaatac cgatatgcat cattgtaccg agtacgccca tgccagcagc
    37741 catcaccagg aagcccagcg ttagcgttgg tttacgtccc cagcggtcaa caaggccgat
    37801 tgcgataaag gtggcaagta cgttggtcag gccgacaatc acggtccccc acatttgctc
    37861 ggtagtgttg gtataacccg ccagttcgaa gattttcggc gcgtaataca tgatgacgtt
    37921 catcccggtg aattgctgca ttacctgcaa cagtacgcca aggaacaccg cgcggcggaa
    37981 gttgctgttc tctttaaaca gcgcccagcc actctgttta acctgcaaac tttcacggat
    38041 ttcatccagt tcgcgtttcg cttccgcgct ggtgtcacgc aggcgtagca gcacgcgttc
    38101 ggcatcaaca aaacggcgct tggcggcaaa ccaacgtggg ctgtctggca ggaagaagac
    38161 accaatcagc agcaaaattg ccgggatgat aatcacaccc agcatccagc gccatgcacc
    38221 ggtgtagctg aaggcggtat cagaaagata agcaccgagg atcccgatag tgatcatcaa
    38281 ctgatacatc gagatcatac tgccgcgaat tttttccggc gcaatttctg acaggtacag
    38341 tggtgcggta taagaggcca cacccaccgc cagccccagc agaacgcggg aaagaatcag
    38401 tacttcaacg tttggcgcag ccgcagagaa cagcgaaccg gcaacaaaca aaattgcgcc
    38461 gatcatcagg ctctttttgc gcccgagttt aaaggagagc cagccgctgc ccaccgcacc
    38521 gactgccgca ccgaacatca tggagcttac gacccattct tgcgtgtgcg aagtaatctg
    38581 gaattcatct gcaataaacg gcagtgcgcc agcaattaca ccgatatcca ggccaaagag
    38641 taatcccgcc agagcggcaa ggaagcagac gaaaaaagtc attgccttgt ttgaccgccc
    38701 ctgtttttta gcgtcaggca tgatgccctc caatatggtt attttttatt gtgaattaag
    38761 ataggtgagt acgacgtaaa aagatgtgaa gcaaatcaca tcagtgtaat cgcttacact
    38821 aacat
//
LOCUS       DBANUU010000030        38791 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000030 DBANUU010000000
VERSION     DBANUU010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.030"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..352)
                     /locus_tag="ACIE68_001507"
     CDS             complement(<1..352)
                     /locus_tag="ACIE68_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5907844.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEII
                     SLLLETTPIKANHLR"
     gene            875..2803
                     /gene="thrS"
                     /locus_tag="ACIE68_001508"
     CDS             875..2803
                     /gene="thrS"
                     /locus_tag="ACIE68_001508"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFX5907845.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2807..3349
                     /gene="infC"
                     /locus_tag="ACIE68_001509"
     CDS             2807..3349
                     /gene="infC"
                     /locus_tag="ACIE68_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFX5907846.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3446..3643
                     /gene="rpmI"
                     /locus_tag="ACIE68_001510"
     CDS             3446..3643
                     /gene="rpmI"
                     /locus_tag="ACIE68_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFX5907847.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3696..4052
                     /gene="rplT"
                     /locus_tag="ACIE68_001511"
     CDS             3696..4052
                     /gene="rplT"
                     /locus_tag="ACIE68_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFX5907848.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4169..4293
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4175..4219
                     /gene="pheM"
                     /locus_tag="ACIE68_001512"
     CDS             4175..4219
                     /gene="pheM"
                     /locus_tag="ACIE68_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFX5907849.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4502..5485
                     /gene="pheS"
                     /locus_tag="ACIE68_001513"
     CDS             4502..5485
                     /gene="pheS"
                     /locus_tag="ACIE68_001513"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFX5907850.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5500..7887
                     /gene="pheT"
                     /locus_tag="ACIE68_001514"
     CDS             5500..7887
                     /gene="pheT"
                     /locus_tag="ACIE68_001514"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFX5907851.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITSEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7892..8191
                     /gene="ihfA"
                     /locus_tag="ACIE68_001515"
     CDS             7892..8191
                     /gene="ihfA"
                     /locus_tag="ACIE68_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFX5907852.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8292..9272
                     /gene="btuC"
                     /locus_tag="ACIE68_001516"
     CDS             8292..9272
                     /gene="btuC"
                     /locus_tag="ACIE68_001516"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFX5907853.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            9335..9886
                     /gene="btuE"
                     /locus_tag="ACIE68_001517"
     CDS             9335..9886
                     /gene="btuE"
                     /locus_tag="ACIE68_001517"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFX5907854.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9886..10635
                     /gene="btuD"
                     /locus_tag="ACIE68_001518"
     CDS             9886..10635
                     /gene="btuD"
                     /locus_tag="ACIE68_001518"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFX5907855.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10713..11177
                     /gene="nlpC"
                     /locus_tag="ACIE68_001519"
     CDS             10713..11177
                     /gene="nlpC"
                     /locus_tag="ACIE68_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFX5907856.1"
                     /translation="MRFCLILITALLLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11424..12137
                     /gene="ydiV"
                     /locus_tag="ACIE68_001520"
     CDS             11424..12137
                     /gene="ydiV"
                     /locus_tag="ACIE68_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFX5907857.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12200..13636
                     /gene="selO"
                     /locus_tag="ACIE68_001521"
     CDS             12200..13636
                     /gene="selO"
                     /locus_tag="ACIE68_001521"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFX5907858.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGERMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(13640..13831)
                     /gene="hemP"
                     /locus_tag="ACIE68_001522"
     CDS             complement(13640..13831)
                     /gene="hemP"
                     /locus_tag="ACIE68_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFX5907859.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13963..15009)
                     /gene="aroH"
                     /locus_tag="ACIE68_001523"
     CDS             complement(13963..15009)
                     /gene="aroH"
                     /locus_tag="ACIE68_001523"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFX5907860.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYYEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(15166..15999)
                     /gene="ppsR"
                     /locus_tag="ACIE68_001524"
     CDS             complement(15166..15999)
                     /gene="ppsR"
                     /locus_tag="ACIE68_001524"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFX5907861.1"
                     /translation="MENAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEVLYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16332..18710
                     /gene="ppsA"
                     /locus_tag="ACIE68_001525"
     CDS             16332..18710
                     /gene="ppsA"
                     /locus_tag="ACIE68_001525"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFX5907862.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18767..20413)
                     /gene="fadK"
                     /locus_tag="ACIE68_001526"
     CDS             complement(18767..20413)
                     /gene="fadK"
                     /locus_tag="ACIE68_001526"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFX5907863.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDARLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGKNISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(20470..20763)
                     /gene="ydiT"
                     /locus_tag="ACIE68_001527"
     CDS             complement(20470..20763)
                     /gene="ydiT"
                     /locus_tag="ACIE68_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFX5907864.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20760..22049)
                     /gene="ydiS"
                     /locus_tag="ACIE68_001528"
     CDS             complement(20760..22049)
                     /gene="ydiS"
                     /locus_tag="ACIE68_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFX5907865.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNNDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMELAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22105..23043)
                     /gene="ydiR"
                     /locus_tag="ACIE68_001529"
     CDS             complement(22105..23043)
                     /gene="ydiR"
                     /locus_tag="ACIE68_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFX5907866.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23063..23827)
                     /gene="ydiQ"
                     /locus_tag="ACIE68_001530"
     CDS             complement(23063..23827)
                     /gene="ydiQ"
                     /locus_tag="ACIE68_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFX5907867.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLTTDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            24143..25054
                     /gene="ydiP"
                     /locus_tag="ACIE68_001531"
     CDS             24143..25054
                     /gene="ydiP"
                     /locus_tag="ACIE68_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFX5907868.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            complement(25097..26248)
                     /gene="ydiO"
                     /locus_tag="ACIE68_001532"
     CDS             complement(25097..26248)
                     /gene="ydiO"
                     /locus_tag="ACIE68_001532"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFX5907869.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(26262..27857)
                     /gene="ydiF"
                     /locus_tag="ACIE68_001533"
     CDS             complement(26262..27857)
                     /gene="ydiF"
                     /locus_tag="ACIE68_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFX5907870.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(28002..28760)
                     /gene="aroD"
                     /locus_tag="ACIE68_001534"
     CDS             complement(28002..28760)
                     /gene="aroD"
                     /locus_tag="ACIE68_001534"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFX5907871.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(28791..29657)
                     /gene="ydiB"
                     /locus_tag="ACIE68_001535"
     CDS             complement(28791..29657)
                     /gene="ydiB"
                     /locus_tag="ACIE68_001535"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFX5907872.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(29669..30934)
                     /gene="ydiN"
                     /locus_tag="ACIE68_001536"
     CDS             complement(29669..30934)
                     /gene="ydiN"
                     /locus_tag="ACIE68_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5907873.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(31161..32375)
                     /gene="ydiM"
                     /locus_tag="ACIE68_001537"
     CDS             complement(31161..32375)
                     /gene="ydiM"
                     /locus_tag="ACIE68_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5907874.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYLCTFPPHPGHRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(32475..32831)
                     /gene="ydiL"
                     /locus_tag="ACIE68_001538"
     CDS             complement(32475..32831)
                     /gene="ydiL"
                     /locus_tag="ACIE68_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFX5907875.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(32967..33074)
                     /gene="rprA"
                     /locus_tag="ACIE68_001539"
     ncRNA           complement(32967..33074)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACIE68_001539"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(33260..34372)
                     /gene="ydiK"
                     /locus_tag="ACIE68_001540"
     CDS             complement(33260..34372)
                     /gene="ydiK"
                     /locus_tag="ACIE68_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFX5907876.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            34761..37817
                     /gene="ydiJ"
                     /locus_tag="ACIE68_001541"
     CDS             34761..37817
                     /gene="ydiJ"
                     /locus_tag="ACIE68_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFX5907877.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            37814..38224
                     /gene="menI"
                     /locus_tag="ACIE68_001542"
     CDS             37814..38224
                     /gene="menI"
                     /locus_tag="ACIE68_001542"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFX5907878.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            38324..38512
                     /gene="ydiH"
                     /locus_tag="ACIE68_001543"
     CDS             38324..38512
                     /gene="ydiH"
                     /locus_tag="ACIE68_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFX5907879.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
BASE COUNT         9813 a         9391 c         9942 g         9645 t
ORIGIN      
        1 ttcgcaaatg gttagcttta ataggcgtgg tttctagtaa taaggaaatg atttcttttt
       61 tatagtgtaa tgtaatgagt atattttctg catggatggg tgaagtggca aagttaatgg
      121 taaaaagggc agtagaataa caattttgtg atatttttcg acagtttgcg gtaaattcct
      181 gagtaacaca gtatgacagc atcgaactta agtcagaggt atcaagtagg gtgatgagtt
      241 ggcgcgcaaa ttgattaata ggctctgatg aaggcgttgg tatgtcgcta tcgatgtgca
      301 ttctatgagg agacttataa tgagttctga taataatgtc gttttgactc ataatctatt
      361 cgccttatat atgtctggag gttttgagta tgatgattaa ttattatcgc cagaacgaaa
      421 tgtgcacgaa aaattgctat aagaattgtg tcacttttat cattgtgata tgaccagttc
      481 gtcattaatc ttatgatcgt ttaaatacta ccgccaaatc atgcaacgaa ctttgctgga
      541 ttcagtttat gctgctgtaa atccgctcgc agtaaacctt tcagacgctc cgtgatgtta
      601 tcagttgttc tttaagcgtt ttgctggtgt actcactaca aacgaattgc gaatcgaatc
      661 aatgtgaaac ggaaaggtac aatctccctt tttttagtgt taacatcgct caaccgggtg
      721 gaaggtgaga ggttaatgtc tttcatttgt gtacctgatt gcgaaccaat ttagcatttg
      781 ttggctagat ggtttcgcaa tgaactgtta ataaacaaat ttttctttgt atgtgatctt
      841 tcgtgtgggt caccactgca aataaggata taaaatgcct gttataactc ttcctgatgg
      901 cagccaacgc cattacgacc acgctgtaag ccccatggat gttgcgctgg acattggtcc
      961 aggtctggcg aaagcctgta tcgcagggcg cgttaatggc gaactggttg atgcttgcga
     1021 tctgattgaa aacgacgcac aactgtcgat cattaccgcc aaagacgaag aaggtctgga
     1081 gatcattcgt cactcctgtg cgcacctgtt agggcacgcg attaaacaac tttggccgca
     1141 taccaaaatg gcaatcggcc cggttattga caacggtttt tattacgacg ttgatcttga
     1201 ccgcacgtta acccaggaag atgtcgaagc actcgagaag cggatgcatg agcttgctga
     1261 gaaaaactac gacgtcatta agaagaaagt cagctggcac gaagcgcgtg aaactttcgc
     1321 caaccgtggg gagagctaca aagtctccat tcttgacgaa aacatcgccc atgatgacaa
     1381 gccaggtctg tacttccatg aagaatatgt cgatatgtgc cgcggtccgc acgtaccgaa
     1441 catgcgtttc tgccatcatt tcaaactaat gaaaacggca ggggcttact ggcgtggcga
     1501 cagcaacaac aaaatgttgc aacgtattta cggtacggcg tgggcagaca aaaaagcact
     1561 taacgcttac ctgcagcgcc tggaagaagc cgcgaaacgc gaccaccgta aaatcggtaa
     1621 acaactcgac ctgtatcata tgcaggaaga agcgccgggt atggtattct ggcacaacga
     1681 cggctggacc attttccgtg aactggaagt gtttgttcgt tctaaactga aagagtacca
     1741 gtatcaggaa gttaaaggtc cgttcatgat ggaccgtgtc ctgtgggaaa aaaccggtca
     1801 ctgggacaac tacaaagatg caatgttcac cacgtcttct gagaaccgtg aatactgcat
     1861 taagccgatg aactgcccgg gtcacgtaca aattttcaac caggggctga agtcttatcg
     1921 cgatctgccg ctgcgtatgg ccgagtttgg tagctgccac cgtaatgagc cgtcaggttc
     1981 gctgcatggc ctgatgcgcg tgcgtggatt tacccaggat gacgcgcata tcttctgtac
     2041 tgaagaacaa attcgcgatg aagttaacgg atgtatccgt ttagtctatg atatgtacag
     2101 cacttttggc ttcgagaaga tcgtcgtcaa actctccact cgtcctgaaa aacgtattgg
     2161 cagcgacgaa atgtgggatc gtgctgaggc ggacctggcg gttgcgctgg aagaaaacaa
     2221 catcccgttt gaatatcaac tgggtgaagg cgctttctac ggtccgaaaa ttgaatttac
     2281 cctgtatgac tgcctcgatc gtgcatggca gtgcggtaca gtacagctgg acttctcctt
     2341 gccgtctcgt ctgagcgcct cctatgtggg tgaagacaac gagcgtaagg taccggtaat
     2401 gattcaccgc gcaattcttg ggtcgatgga acgtttcatc ggtatcctga ccgaagaatt
     2461 cgctggtttc ttcccgacct ggcttgcgcc ggttcaggtt gttatcatga atattaccga
     2521 ttcacagtct gattacgtta acgaattgac gcaaaaacta tcaaatgcgg gcattcgtgt
     2581 taaagcagac ttgagaaatg agaagattgg ctttaaaatc cgcgagcaca ctttgcgtcg
     2641 cgtcccatat atgctggtct gtggtgataa agaggtggaa tcaggcaaag ttgccgttcg
     2701 cacccgccgt ggtaaagacc tgggaagcat ggacgtaaat gaagtgatcg agaagctgca
     2761 acaagagatt cgcagccgca gtcttaaaca attggaggaa taaggtatta aaggcggaaa
     2821 acgagttcaa acggcgcgcc ctaaccgtat caatggcgaa attcgcgccc aggaagttcg
     2881 cttaacaggt ctggaaggcg agcagcttgg tattgtgagt ctgagagaag ctctggagaa
     2941 agcagaagaa gccggagtag acttagtcga gatcagccct aacgccgagc cgccggtttg
     3001 tcgtataatg gattacggca aattcctcta tgaaaagagc aagtcttcta aggaacagaa
     3061 gaaaaagcaa aaagttatcc aggttaagga aattaaattc cgtcctggta cagatgaagg
     3121 cgactatcag gtaaaactcc gcagcctgat tcgctttctc gaagagggtg ataaagccaa
     3181 aatcacgctg cgtttccgcg gtcgtgagat ggcgcaccag caaatcggta tggaagtgct
     3241 taatcgcgtg aaagacgatt tgcaagaact ggcggtggtc gaatccttcc caacgaagat
     3301 cgaaggccgc cagatgatca tggtgctcgc tcctaagaag aaacagtaag gccttcaagt
     3361 agcactgtct gtggagcctt cgggcttcac aggcattgtt cgcctttgtt tcgtttatta
     3421 acaatgcgaa gtggaagtta ttaaaatgcc aaaaattaag accgtacgcg gtgctgctaa
     3481 gcgcttcaaa aaaaccggta aaggtggttt taagcacaag cacgctaacc tgcgtcacat
     3541 tctgaccaaa aaagcgacca aacgtaaacg tcacctgcgt ccgaaagcca tggtttccaa
     3601 aggcgatctg ggcctggtaa tcgcgtgcct gccgtacgca taagccgtta acgtttttaa
     3661 ctttttaatt agaatataga tacaggagag cacatatggc tcgcgtaaaa cgtggtgtta
     3721 ttgcacgcgc acgtcacaag aaaattttga aacaagctaa aggctactac ggtgcgcgtt
     3781 ctcgcgtata ccgcgttgcc ttccaggctg ttatcaaagc tggtcagtat gcttaccgtg
     3841 accgtcgtca acgtaagcgt cagttccgtc aactgtggat tgcgcgtatc aacgcagcag
     3901 cacgtcagaa cggtatttct tacagcaaat tcatcaatgg cctgaaaaaa gcctctgttg
     3961 aaatcgaccg taagatcctg gctgatatcg cagtattcga caaagtagcg ttcaccgctc
     4021 tggttgaaaa agcgaaagca gctctggcat aagccagttg aaagagggag ctagtctccc
     4081 tcttttcgtt tcaacgccat caaaacattg acttttatcg ccgtagcctt ttcaataaag
     4141 gtcttttgaa gagtaccaaa aggtaacgca agcaatgaat gctgctattt tccgcttctt
     4201 tttttacttt agcacctgaa tccaggaggc tagcgcgtga gaagagaaac ggaaaacagc
     4261 gcctgaaagc ctcccagtgg aggctttttt tgtatgcgcg ttttgaaaaa taaaaagcca
     4321 gtctatcagg cgtaattgtc gcagccagtc tggacacggc cagcgcggag aaaccggagc
     4381 gtaattaagt acgtgaggat ttcgagcact gcccgggacc aaaatggcaa gtaaaatagc
     4441 ctgatgggat aggctctaag tccaacgaac cagtgtcacc actgacacaa tgaggaaaac
     4501 catgtcacat ctcgcagaac tggttgccag tgcgaaggcg gccattagcc aggcgtcaga
     4561 tgttgccgcg ttagataatg tgcgcgtcga atatttgggt aaaaaagggc acttaaccct
     4621 tcagatgacg accctgcgtg agctgccgcc agaagagcgt ccggcagctg gtgcggttat
     4681 caacgaagcg aaagagcagg ttcagcaggc gctgaatgcg cgtaaagcgg aactggaaag
     4741 cgctgcactg aatgcgcgtc tggcggcgga aacgattgat gtctctctgc caggtcgtcg
     4801 cattgaaaac ggcggtctgc atccggttac ccgtaccatc gaccgtatcg aaagtttctt
     4861 cggtgagctt ggctttaccg tggcaaccgg gccggaaatc gaagacgatt atcataactt
     4921 cgatgctctg aacattcctg gtcaccaccc ggcgcgcgct gaccacgaca ctttctggtt
     4981 tgacgctacc cgcctgctgc gtacccagac ctctggcgta cagatccgca ccatgaaagc
     5041 ccagcagcca ccgattcgta tcatcgcgcc tggccgtgtt tatcgtaacg actacgacca
     5101 gactcacacg ccgatgttcc atcagatgga aggtctgatt gttgatacca acatcagctt
     5161 taccaacctg aaaggcacgc tgcacgactt cctgcgtaac ttctttgagg aagatttgca
     5221 gattcgcttc cgtccttcct acttcccgtt taccgaacct tctgcagaag tggatgtcat
     5281 gggtaaaaac ggtaaatggc tggaagtgct gggctgcggg atggtgcatc cgaacgtgct
     5341 gcgtaacgtt ggcatcgacc cggaagttta ctctggtttc gccttcggga tggggatgga
     5401 gcgtctgact atgttgcgtt acggcgtcac cgacctgcgt tcattcttcg aaaacgatct
     5461 gcgtttcctc aaacagttta aataaggcag gaatagatta tgaaattcag tgaactgtgg
     5521 ttacgcgaat gggtgaaccc ggcgattgat agcgatgcgc tggcaaatca aatcactatg
     5581 gcgggcctgg aagttgacgg tgtagaaccg gttgccggta gcttccacgg cgtggtagtt
     5641 ggtgaagtgg ttgagtgtgc gcagcatccg aacgctgaca aactgcgtgt gacaaaagtg
     5701 aatgttggcg gcgatcgcct gctggacatc gtctgcggtg cgccaaactg ccgtcagggc
     5761 ctgcgtgtgg cggtagcgac cattggtgct gttctgccgg gtgatttcaa aattaaagcg
     5821 gcgaaactgc gtggtgaacc gtctgaaggg atgctgtgct ccttttctga gctgggaatt
     5881 tctgacgatc atagcggcat tatcgagctg cctgcggatg cgccgattgg caccgacatc
     5941 cgtgaatacc tgaaacttga tgacaacacc atcgaaatca gcgtgacgcc aaaccgtgcc
     6001 gactgcttag gcatcattgg tgttgcgcgt gacgttgccg tgctgaacca gctgccgctg
     6061 gttgaaccgg aaatcgttcc ggttggtgcg accatcgacg acacgctgcc gattacagtc
     6121 gaagcgccgg aagcctgccc gcgttatctt ggccgtgtgg taaaaggcat taacgttaaa
     6181 gcgccaactc cgctgtggat gaaagaaaaa ctgcgtcgtt gcgggatccg ttctatcgat
     6241 gcagttgttg acgtcaccaa ctatgtgctg ctcgaactgg gccagccgat gcacgctttc
     6301 gataaagatc gcattgaagg cggcattgtg gtgcggatgg caaaagaggg cgaaacgctg
     6361 gtgctgcttg atggcactga agcgaagctg aatgctgaca ctctggtcat cgccgaccac
     6421 aacaaggcgc tggcgatggg cggcatcttt ggtggcgaac actctggcgt gaatgacgaa
     6481 acgcaaaacg tcctgctgga atgcgctttc ttcagcccgc tgtctatcac cggtcgtgct
     6541 cgtcgtcatg gcctgcatac tgatgcgtct caccgttatg agcgtggcgt tgatccggca
     6601 ctgcagcaca aagcgatgga acgtgcgacc cgtctgctga tcgacatctg cggtggtgag
     6661 gctggtccgg taattgatat caccagcgaa gcaacgctgc cgaagcgtgc aaccatcact
     6721 ttacgtcgta gcaaactgga tcgcctgatc ggccatcata ttgcggatga gcaggtaact
     6781 gacattctgc gtcgtctcgg ctgcgaagtg accgaaggca aagacgagtg gcaggcagtt
     6841 gcgccgagct ggcgtttcga tatggagatt gaagaagatc tggtcgaaga agtcgcgcgt
     6901 gtttacggct acaacaacat cccggatgag ccggtacagg caagcctgat tatgggtact
     6961 caccgtgaag ctgacctgtc gctcaagcgc gtgaaaacgc tgctcaacga caaaggctat
     7021 caggaagtga tcacctacag cttcgttgat ccgaaagtgc agcagatgat ccatccaggc
     7081 gttgaagcct tactgctgcc aagcccgatc tctgttgaaa tgtcagcaat gcgtctttct
     7141 ctgtggaccg gcctgctggc aaccgtggtg tacaaccaga accgtcagca gaaccgtgtg
     7201 cgcattttcg aaagcggtct gcgtttcgta ccagatactc aagcaccgtt gggcattcgt
     7261 caggatctga tgttagccgg tgtgatttgc ggtaaccgtt acgaagagca ctggaacctg
     7321 gcaaaagaga ccgttgattt ctatgatttg aaaggcgatc ttgaatccgt tctcgacctg
     7381 accggtaaac tgaatgaggt tgagttccgt gcagaagcga atccggcact gcatccgggg
     7441 caatccgcag cgatttatct gaaaggtgaa cgtattggtt ttgttggggt tgttcatcct
     7501 gaactggaac gtaaactgga tcttaacggt cgcactctgg tgttcgaact ggagtggaac
     7561 aagctcgcag accgcgtggt gcctcaggcg cgcgagattt ctcgcttccc ggcgaaccgt
     7621 cgtgacatcg cggtggtggt cgcagaaaac gttcccgcag cggatatttt atccgaatgt
     7681 aagaaagttg gcgtaaatca ggtagttggc gtaaacttat ttgacgtgta ccgcggtaag
     7741 ggtgttgcgg aggggtataa gagcctcgcc ataagcctga tcctgcaaga taccagccgt
     7801 acactcgaag aagaggagat tgccgctacc gtcgccaaat gtgtagaggc attaaaagag
     7861 cgattccagg catcattgag ggattgaacc tatggcgctt acaaaagctg aaatgtcaga
     7921 atatctgttt gataagcttg ggcttagcaa gcgggatgcc aaagaactgg ttgaactgtt
     7981 tttcgaagag atccgtcgcg ctctggaaaa cggcgaacag gtgaaactct ctggttttgg
     8041 taacttcgat ctgcgtgata agaatcaacg cccgggacgt aacccgaaaa cgggcgagga
     8101 tattcccatt acagcacggc gcgtggtgac cttcagaccc gggcagaagt taaaaagccg
     8161 ggtcgaaaac gcttcgccca aagacgagta atctgatcta actaaaaagg ccgctctgcg
     8221 gccttttttc ttttcactgt cgaagagtca ccgtaaaatc aacgccatga cacttcagca
     8281 gaacggatac catgctgaca cttgcccgcc aacaacagcg acaaaatatt cgctggttat
     8341 tatgcctgtc agttttgatg ctgctggcgc ttctcttaag cctttgcgcc ggtgaacaat
     8401 ggatctcgcc aggtgactgg tttactcctc gtggcgaact gttcgtctgg caaattcgcc
     8461 tgccacgtac gctggctgta ttgctggttg gtgcggcgct ggctatatcc ggcgctgtaa
     8521 tgcaggcgtt gtttgaaaat cctctggcag aacctggact acttggcgtc tctaacggcg
     8581 caggcgtggg gcttatcgcc gcggtattgc ttgggcaagg gcaactcccc aactgggcgc
     8641 tagggctgtg tgcgattgct ggcgcgctta tcatcacttt aatactctta cgtttcgccc
     8701 gtcgtcatct ttcgaccagt cggttattgc tggctggcgt tgcattaggg attatctgta
     8761 gcgcactaat gacgtgggct atctactttt ccacctcagt tgatttgcgt cagctgatgt
     8821 actggatgat gggcggtttt ggcggcgtag actggcggca aagctggctg atgctggcat
     8881 tgatccccgt gttgttgtgg atctgttgtc agtccaggcc gatgaatatg ttagcacttg
     8941 gcgagatctc ggcgcggcaa ctgggtttac ccctgtggtt ctggcgcaat gtgctggtgg
     9001 cagcgaccgg ctggatggtt ggcgtcagtg tggcgctggc gggtgctatc ggctttattg
     9061 gtctggtgat cccacatatt ctccggctgt gtggtttaac cgatcatcgc gtattacttc
     9121 ccggctgcgc gctggcaggg gcgagcgcat tgctgctggc cgatattgta gcgcgcctgg
     9181 cattagctgc cgcagagctg cctattggcg tggtcaccgc aacgttgggt gcgccggtgt
     9241 ttatctggtt attgttaaaa gcaagacgtt agccgcaaaa agacggtcta tgattaaaag
     9301 ctaagatttt tactgaccac acccaggaga aacgatgcaa gattccattc tgacgaccgt
     9361 agtgaaagat atcgacggtg aagtgaccac gctggagaag tacgccggta atgtgctgtt
     9421 gattgtcaat gtcgcctcaa agtgtggctt aacgccgcaa tatgagcagt tggagaatat
     9481 tcagaaagcc tgggccgatc gaggttttgt ggtgctggga ttcccgtgca accagtttct
     9541 ggaacaagaa ccgggcagcg atgaagagat taaaacttac tgtaccacca catggggggt
     9601 gacgttcccg atgttcagta agattgaagt taatggcgaa ggacgccatc cgctgtatca
     9661 aaaattgatt gccgcagcgc cgaccgcagt cgcgccggaa gagagcggat tctatgcccg
     9721 tatggtcagc aaaggccgtg caccgctgta cccggatgat attttatgga attttgaaaa
     9781 attcctggtt ggcagggacg gaaaagtcat ccagcgtttt tccccggata tgacgccgga
     9841 agatcccatt gtgatggaaa gcattaaact ggcgttggca aaataatgtc tattgtgatg
     9901 cagttacaag atgttgcgga atctacccgc ctggggccgc tttctggcga ggttcgggct
     9961 ggggagatcc tgcacctggt ggggccgaat ggcgcgggta agagtacctt actggcgcga
    10021 atggccggaa tgaccagcgg taagggaagc attcagttcg cggggcaacc actggaagca
    10081 tggtccgcaa caaaactcgc gctgcatcgc gcctatcttt cacaacagca gacgccgccg
    10141 tttgcaatgc cggtctggca ctacctgaca ctgcatcagc acgataaaac gcgtaccgaa
    10201 ctactgaatg atgtcgcagg ggcgctggct cttgatgaca aactcggacg tagcaccaat
    10261 caactttccg gcggtgaatg gcaacgcgta cgtcttgctg cggtggtgtt gcaaatcaca
    10321 ccacaagcca atcccgcagg ccaattgctg cttcttgatg agccgatgaa cagtcttgat
    10381 gttgcgcaac aaagtgcgtt agacaaaatt ctgagcgcgc tgtgtcagca aggactggcg
    10441 attgtgatga gcagtcacga tctcaaccac acattgcgtc atgcgcatcg ggcgtggttg
    10501 ctaaaaggtg gaaaaatgct ggccagtgga cgcagggaag aggtgctcac gccgccaaat
    10561 ctggcgcagg cctatgggat gaattttcgc cgtctggata tcgaaggtca cagaatgctg
    10621 atttcgacca tctgatttca ttagtatctt gaaaatcggg taattacgac gctaaattaa
    10681 tgcaagaata aaaaacagag gaaccgtacg gaatgcgttt ctgccttatt ttgatcacag
    10741 cactgcttct ggccgggtgt agccaccata aagcaccgcc gccaaatgcc agactttctg
    10801 attcgattac cgttattgcc ggtttgaacg accagctaca aagctggcat ggcacgccgt
    10861 atcgttatgg tggcatgacg cggcgcggtg tggactgttc gggatttgtg gttgtgacga
    10921 tgcgcgatcg tttcgatttg cagctgcccc gtgaaaccaa acaacaagcc tctattggca
    10981 cgcaaattga taaagacgag ttgctgccag gtgacctggt ctttttcaaa acggggtccg
    11041 gacaaaatgg tttgcatgta ggtatttacg ataccaacaa ccaatttatc cacgcctcta
    11101 ccagcaaggg agtgatgcgt tcctcacttg ataatgtcta ttggcagaaa aatttctggc
    11161 aggcgaggcg aatctagtat gtcgaaaaat ggacggcgat gatacatcgc cgtccattta
    11221 ctcctgaata cagtagtatt ttgcagacaa aaattccgta aatggattat tcgtttataa
    11281 tcaggataat ctaaaaaaag gacacctgaa aaagtggtta acatggaaca aatgaaagtt
    11341 actccttctg taattattat gtcttatggc ctcatccctt ttagccggat actgaaaaac
    11401 atccttcgag agggacggtt accatgaaga tttttttgga gaatctttat cattctgatt
    11461 gctatttcct gccgataaga gacaaccagc aagtccttgt cggcgttgag ttaattaccc
    11521 atttctccag tgaagatggc acggtgcgta tcccgacgag tcgggttata gcgcaactta
    11581 ccgaggagca acattggcaa ttattttcag agcagttgga attactaaaa tcatgccaac
    11641 atttttttat tcaacataaa ctctttgcct ggcttaattt aacgccacaa gtggctacat
    11701 tattattaga gagggataat tacgcaggcg aactattaaa atatccgttt attgaactgt
    11761 tgattaatga gaattacccg catctcaatg aaggaaaaga taatcgcggt cttttatcct
    11821 tatcgcaagt gtatcccctt gtgttaggta atttgggagc aggcaatagc acaatgaaag
    11881 cggtttttga tggtcttttc acccgcgtca tgctggataa aagttttatt cagcaacaaa
    11941 tcacgcaccg ctccttcgaa ccgtttattc gtgcgatcca ggcgcaaatt tccccttgtt
    12001 gcaattgcat cattgcggga ggcattgata cggcagaaat attggcacag attaccccct
    12061 tcgatttcca tgctctccag ggatgcctgt ggcctgccgt tcccataaat cagataacga
    12121 cgttggttca gcgataaccc ttctgtttgc tggtgtttaa gacgagagta accgtctaca
    12181 ctatcaaaca ggaggatcta tgaccctgtc ttttgttacc cgctggcgcg atgaattgcc
    12241 agaaacctat acagcacttt cccctacgcc tttaaataat gcccggctga tttggcataa
    12301 taccgaactg gctaacacgc tgagtattcc atcgtcgctg tttaaaaatg gcgcaggtgt
    12361 ctggggcggc gaagccctac tgcctggtat gtcaccactg gcgcaggttt acagtggtca
    12421 tcagttcggc gtctgggcgg gccaactggg tgatgggcgc ggcattttac tcggcgaaca
    12481 actgcttgct gatggcacta caatggactg gcatctgaaa ggtgctggcc tgacgcctta
    12541 ttcgcgaatg ggtgatggac gggcggtttt acgttcgacg atacgagaaa gtctcgccag
    12601 tgaggcgatg cattatctgg gcattccgac gacccgcgcg ttaagtatcg tcaccagcga
    12661 ttcgccagtg tatcgggaaa cggcggagcc aggcgcgatg ctgatgcgtg tggcaccaag
    12721 tcatctgcgc tttggtcatt tcgaacattt ttactaccgc cgcgagtcgg aaaaggttcg
    12781 tcagttagct gactttgcta ttcgtcatta ctggtcacat cttgaagatg atgaggacaa
    12841 ataccgtctc tggtttagcg atgttgtcgc acgtaccgcg tcgttaattg cccaatggca
    12901 gacggtcggc tttgctcatg gggtgatgaa taccgacaac atgtcgctgc tggggctgac
    12961 gcttgattac gggccgtttg gttttcttga tgattacgaa cccggtttta tttgtaatca
    13021 ctcggatcat caagggcgtt acagctttga taatcaacct gccgtcgcgt tgtggaattt
    13081 acagcgtctg gcgcagacat tgtcgccatt tgttgccgta gatgccctga atgaggccct
    13141 ggacagctat cagcaggttt tgttgacgca ttatggagaa cggatgcggc agaaactggg
    13201 cttcatgacg gagcaaaaag aggataacgc gctactgaat gaattattca gtctgatggc
    13261 gcgagagcgc agcgattata cccgcacatt ccgcatgctg agtctgaccg agcagcacag
    13321 cgcggcgtca ccgctacgtg atgagtttat tgatcgtgcg gcatttgatg actggtttgc
    13381 ccgttatcgg gggcgtttgc aacaagacga ggttagcgat agtgagcgtc agcagctgat
    13441 gcaaagcgtt aacccagctc tggtgttgcg caactggttg gcgcaacggg cgattgaagc
    13501 ggcagaaaag ggtgatatga cggaattgca ccgcctgcat gaggcgttgc gaaatccttt
    13561 cagcgacaga gctgatgact atgtcagtcg tccacctgac tggggtaaac ggctggaagt
    13621 cagctgctcg agttaaaggc tacttggtca acagcagctt gccagcctga gttttacgga
    13681 gcagatattc ttgcccgtca tgatcgataa tcagtttgcc atccggacct aacagtgtct
    13741 ggctggaaat tcgccgaata ggctgcgggg aagcggtctt gtgattggca tccgtttcag
    13801 gcgtgagttt tctgctatcc gtataacgca taaaaattaa taataattga tataacaatg
    13861 attcttatta tcaatattat ggctgaccag agcaagcagt aatgtgctga gaagacaaca
    13921 agttgcgcag aaatgcccat cccatcagga atgggcacgc attcagaagc gggtatctac
    13981 cgcagaggcg agtttttcga ccaggcgttc ggtatcttcc cagcccagac acgggtcggt
    14041 aatggattga ccgtaagtga gcggctgacc gccgacgatt ttttgcgttc cttcgcgcag
    14101 gaaactttcc gccataattc cagcaatcgc cgtagagcca ttgcggattt gctgacaaat
    14161 atcctcacaa acttctaact ggcgacggtg ctgcttctgg cagttaccgt ggctgaaatc
    14221 caccaccaga tgttcaggta aatcaaactc gtgcagcgta tcgcaggctg cggcgatatc
    14281 atcggcatga taattcggtt ttttgccgcc acgcataata atgtggccat acgggttgcc
    14341 gctggtctga tagatggtca tctgaccatt tttgtctggc gagagaaaca tatggctggc
    14401 gcgggctgcg cggatagcat ccacggcaat ccgcgtgttg ccatcggtac catttttaaa
    14461 acctaccgga caggagagtg ccgaagccat ttcacggtgg atctgacttt cggtagtacg
    14521 tgcgccaatc gctccccaac tgattaaatc agcaataaac tgaccggtca ccatatcgag
    14581 gaactcagtc gcggtaggga cacccagctc atttacctgt aaaagtaatt tgcgcgccag
    14641 ctccagaccg tgatttaccc gatagctgcc gtttaaatct ggatcggaga ttagcccttt
    14701 ccagccgaca acagttcggg gtttttcata ataggtgcgc attacgattt caagccgtga
    14761 ctggtactgg ttgcgcagcg actgcagacg ggtggcgtat tccattgcag cggtgagatc
    14821 gtggatcgag caggggccaa taatgaccaa cagtcgctta tcttcaccat tcagtatttt
    14881 ttcaattctg cggcgggagt cggtgacatg ggtggcgacg ccaggcgtta cgggataccg
    14941 tagcgcgagt tcggcgggcg ttaccaggct ctcaatacgc gcagtacgga gttcgtcagt
    15001 tctgttcatt acaagtctca gaaatttgtt gcttctacgg caggactacc gggaagtgat
    15061 gcgcatcacg ataaaccaat cgcagctgat ttcaagtgcg aggtgtgtca cacctcgccg
    15121 ggtggttagc atgataacaa aaaaacaagt taatgcacta gttctctagt acattcgacg
    15181 gctaaggccc atgatatcga ggatcttggt ggcaatctct tctaccgaat aattggtact
    15241 gttaatccac gggatctgat ttttacggta caacacttcc acttccgcga cttccatcct
    15301 gcactgacga agcgaggcat agcgactgtt ctcccgacgt tcctcgcgaa tcgccgccag
    15361 tcgttccggg tcgatagtca ggccgaacaa tttatgctga agcggtttga gcgacgcggg
    15421 tagcaccaga ttatccatat cgtcggcaat aaaggggtag tttgccgcgc ggataccaaa
    15481 ctgcattgcc agatacagac tggtgggggt tttaccacag cgagaaacac cgagcaggat
    15541 cacctgagcc tggtccagat tgcgcaacga aatgccgtca tcgtgggcga gggtgtaatc
    15601 aatcgccgca atgcgcgcat catatttatt gagattatta gggttaaggc catgggtacg
    15661 atgagcaatc ggcgttggat ccagtttcat ctcttgttgt agcggggcaa ccagcgcctg
    15721 aacgatatcc tggcaaaaac cttcactttg caagatgatg gcgcgaatct ccggcaacac
    15781 gatggagtag aagaccagcg ggcgcacacc tgtctggtga taaattgcgt caatctgatc
    15841 cttcactgcc cgtgcacggc tctcattttc gacaaacggc agcgtaatgc tgctgatagt
    15901 gacgggaaat tgtgacatta ctgcgtgtcc taatacctcc gcagttattg ccgtaccatc
    15961 agaaatataa aaaacgtggc gatcaacagc attttccatt ttgttcttcc cgtgatgcag
    16021 acataattct atgaaagcat aaattaaaaa cgcacagaag cgtaggacgt aatgtctggt
    16081 ttataaaatg aaccttcaat tttatttttt atgaaaacag catttcattt ttatggtttc
    16141 gtttataccg atggtttatg tggaaattgt cgaatagagc aggtttgcgc aacgctggga
    16201 tcagtcttaa aaagtaaaaa aatatatttg cttgaacgat tcaccgtttt tttcatccgg
    16261 ttaaatatgc aaagataaat gcgcagaaat gtgtttctca aaccgttcat ttatcacaaa
    16321 aggattgttc gatgtccaac aatggctcgt caccgctggt gctttggtat aaccaactcg
    16381 gcatgaatga tgtagacagg gttgggggca aaaatgcctc cctgggtgaa atgattacta
    16441 atctttccgg aatgggcgtt tccgttccga atggtttcgc cacaaccgcc gacgcgttta
    16501 accagtttct ggaccaaagc ggcgtaaacc agcgcattta tgaactgctg gataaaacgg
    16561 atattgacga tgttacccag cttgcgaaag cgggcgcgca aatccgccag tggattatcg
    16621 acactccctt ccagcctgag ctggaaaatg ccatccgcga agcttatgca cagctttccg
    16681 ccgatgacga aaacgcctct tttgcggtgc gctcctccgc tactgcagaa gatatgccgg
    16741 acgcttcctt tgccggtcag caggaaacct tcctcaacgt tcagggtttt gacgccgttc
    16801 tcgtggcagt gaaacatgta tttgcttctc tgtttaacga tcgcgccatc tcttatcgtg
    16861 tgcaccaggg ttacgatcac cgtggcgtgg cgctctccgc cggtgttcaa cggatggtgc
    16921 gctctgacct cgcatcttct ggcgtgatgt tctccattga taccgaatcc ggctttgacc
    16981 aggtggtgtt tatcacttcc gcatggggcc ttggtgaaat ggtcgtgcag ggcgcggtta
    17041 acccggatga gttttacgtc cataaaccga cacttgcagc gaatcgcccg gctatcgtgc
    17101 gtcgcaccat ggggtcgaaa aaaatccgca tggtttacgc gccgacccag gagcacggca
    17161 agcaggttaa aatcgaagac gtaccgcagg aacagcgtga catcttctcg ctgaccaacg
    17221 aagaagtgca ggaactggca aaacaggccg tacaaattga gaaacactac ggtcgcccga
    17281 tggatattga gtgggcgaaa gatggccaca ccggcaaact gttcattgtg caggcgcgtc
    17341 cggaaaccgt gcgctcacgc ggtcaggtca tggagcgtta tacgctgcat tcacagggta
    17401 agattatcgc cgaaggccgt gctatcggtc atcgcatcgg cgcgggtccg gtgaaagtca
    17461 tccatgacat cagcgaaatg aaccgcatcg aacctggcga cgtgctggtc actgacatga
    17521 ccgacccgga ctgggaaccg atcatgaaga aagcatctgc catcgtcacc aaccgtggcg
    17581 gtcgtacctg tcacgcggcg atcatcgctc gtgaactggg cattccggcg gtagtgggct
    17641 gtggtgatgc aacagaacgg atgaaagacg gtgagaacgt cactgtttct tgtgccgaag
    17701 gtgataccgg ttacgtctat gcggagttgc tggaatttag cgtgaaaagc tccagcgtag
    17761 aaacgatgcc ggatctgccg ttgaaagtga tgatgaacgt cggtaacccg gaccgtgctt
    17821 tcgacttcgc ctgcctgccg aacgaaggcg tgggccttgc gcgtctggaa tttatcatca
    17881 accgtatgat tggcgtccac ccacgcgcac tgcttgagtt tgacgatcag gaaccgcagt
    17941 tgcaaaacga aatccgcgag atgatgaaag gttttgattc tccgcgtgaa ttttacgttg
    18001 gtcgtctgac tgaagggatc gcgacgctgg gtgccgcgtt ttatccgaag cgcgtcattg
    18061 tccgtctctc tgattttaaa tcgaacgaat atgccaacct ggtcggtggt gagcgttacg
    18121 agccagatga agagaacccg atgctcggct tccgtggcgc gggccgctat gtttccgaca
    18181 gcttccgcga ctgcttcgcg ctggagtgtg aagcagtgaa acgagtgcgc aacgacatgg
    18241 ggctgaccaa cgttgagatc atgatcccgt tcgtgcgtac cgtagatcag gcgaaagcgg
    18301 tggttgaaga actggcgcgt caggggctga aacgtggcga gaacgggctg aaaatcatca
    18361 tgatgtgtga aattccgtcc aacgccttgc tggcagagca gttcctcgaa tatttcgacg
    18421 gcttctcaat tggctcaaac gatatgacac agctggcgct cggtctggat cgtgactccg
    18481 gcgtggtgtc tgaattgttc gatgagcgca acgatgcggt gaaagcattg ctgtcgatgg
    18541 ctatccgtgc cgcgaagaaa cagggcaaat atgtcgggat ttgcggtcag ggtccgtccg
    18601 accatgaaga ctttgccgca tggttgatgg aagaggggat cgatagcctg tctctgaacc
    18661 cggacaccgt ggtgcaaacc tggttaagcc tggctgaact gaagaaataa aataaatccc
    18721 cggcggcgtt cagtcgccgg ggttatgtga tccccgaaga tgatacttat tcaatctctt
    18781 cacagacatc ctgcgttaat cgccgcataa tatcttttct taacaaaaac ttttgtattt
    18841 tacctgaggc agtgcgcggt agtttttcga ttaccacgat atgttcagga tatttatatt
    18901 ttgcgacccg tttacggcta aaaaaagcca ccacctcttc cagcgataat gaatgatgcg
    18961 gcgctttcag cacgacataa gcgcatgatc gttcacctaa acgttcatcg ggcattgcaa
    19021 ccacacaggc atcgtgaatt ttaggatgct gcaataaaat atcttccact tcacggctgc
    19081 taatgttttt gccgccgcgg acaataatat cttttttgcg tccggtaatt tttatatagc
    19141 cagcctcatc catgcggcag agatcgccgc tgtaatacca gccttcttcg tccagcgcac
    19201 gggcggttaa ttcaggttca tcaaaatacc ccataaacac attggggcca cgcgaggctt
    19261 cttcaccttc gcaacctggc ggtaaggtct tgcgtgcgtc atcgaccact ttaatctcta
    19321 cacctgcggc agcgtaacca tcggtgtgca taaagcgcga caaaggatca tcgagattca
    19381 ccaccgcatg cggcgaactt tctgtggaac cataaacact taataattta atgccgcgct
    19441 gctggcattc acgtgccact tttttgggga ttgtggtacc gccgcaaaga aagaaacgca
    19501 gcgctgaaag gtccgcgggt tgtttctcta gtacattcaa aagatcatag acaaacggcg
    19561 ttgcgcctag catacaggtg caacgctgct gctcaagcag cgcgagacgc gcatcaggag
    19621 tgaaaatatc taacaacacg ctgcgagcgc caattaagaa tggtgccgtt acgccatgca
    19681 gaaagcccgt tgcgtgacca agtggcgcag gcatcataaa gacatcctgc caggtcagat
    19741 tcagtcgcgc gcaataagcc cgctcactgg cgagaatatt gttatgcgtt agcatcacgc
    19801 cctttggcag accctcggtt ccggaggtaa acagcaccgc agctaattca tcgccgtggg
    19861 tcgttatcgc cgtggtcagt gaggtattgt cggcgataat ctgacttaat gagagggaag
    19921 aggtggcggg agccagtttg tccacaccga caatttgttg tagttgtgga agctgatttt
    19981 gcagcggcag gattaaatct accggacgcg tttgtttaaa caacgtcggt gcaaagaaca
    20041 tttttgcctg acacttattg agcacccaca ccagttctgc ttcccgccag gaaggcaaca
    20101 gcggcacgga aaccgcaccg gttttcaggc aggcaagata gataacggta aattcacacc
    20161 agcctggcag ttgaaatgcg atgcgatcgc ctgattcaat acccttcgct aacatccagt
    20221 ttgccagaca gctcgcggcg tgatcgagcg cgctataggt gtacgatgct ccatgattat
    20281 cgaccacggc aattttgtct ggcatcgcac gagcggtctg ctgccagtaa tcggccagcg
    20341 aagcatcgcc ccatagccct tgctgacgat acgccgcacg acgttgttcg ttgaacgtta
    20401 atgtcacttt catgttggac tctcgaaaca gaacatcagg cccgagatgc gggcctgtgg
    20461 gatgcatgat tagccgtagc gaaaatcaat accgaaggtg cctgcgggat attgccactg
    20521 ttcgagaata gtgttaccgc acagcacccg acaggtgccg cactccagac aaccggcaga
    20581 atcaaaatga atgttgccgg cgtcatcctg cttataaagt ccggcagggc aggctttcat
    20641 taatttacgg aattcattga tatcgggatt tgccgccaga atgatatgcg ggtggccctc
    20701 atcaacatgg aatttattga cgcctaattt gatgtcgacg ttaaccgtag cgttctggct
    20761 catagcgcgg ttgctccctt aatgccatct ttcagcaagt tgatcagccc aattttcttc
    20821 gcatgtccca tgatcatttt gcgtaccggc tggtttggtt tgccgtcaat ggtgaacatc
    20881 tcgttcatga tgtcggcgac cattcgtggg tattggctaa acaggcgcgg gttttccatc
    20941 agcgccggga tcttgcggaa atgctgcata tcgcgcatga cgcagctttg ttccagctca
    21001 cgtttgtatt gcgccagact gctggcggag aaatcctcgc gttctttggc ggcgatcacc
    21061 gttgtggcgg cagcctgagc cgatgcaatg gctaactcca tgccacgaac tgtaaaaccc
    21121 aaattcaggc agaagcctgc ggcgtcacca acgatcatca cgccatcgtt aaccagctgc
    21181 ggcaccattg ccagaccgcc ttctggcacc atatgcgcgg aatattcaag cagtttgccg
    21241 ccgctaatta gtgggcgaat ggcggggtgt tgtttaaaat cttccagcat ttgcggcacg
    21301 cttttttgcg catgggcgat atcacccaat ccacaaacca gccccaacga tacggaatcg
    21361 ttgttggtat agaggaatcc gccacccatc aggccgtcag aaggggaacc ggcaaacagc
    21421 caggcggcac cttcctcgcc cgtaatatta aagcgatcgt tgatctgttc tggcgtgagg
    21481 ccaataacct ctttaacacc aacagcgtaa tgatgcggat cggaagcggg aaccattccc
    21541 agcgagcggc caagcatcga gttaacgcca tcagccagaa tcaccacatt cgcttcgaga
    21601 atatcatccc cagcctgcac gccagttacc ttgtttcctt cacgaaccag cgcatcgacg
    21661 cgaacgcctg ggataaactg cgcgcccgcc tgctcggctt gttccatcaa ccacgggtcc
    21721 agacgattac gcaatacggt ataagatgcg tgttgcggaa catctggctg ctcgcggtga
    21781 aaatcgaggg taacggcgct ttcttcggtt aagaaggaga ttttctcgcg tgtgaccttg
    21841 cgttctaccg gcgctgatgc tgcaaagcct ggaatgattg cttcaagtgt gtgggcataa
    21901 agacgcccgc cggtcatgtt tttacatccg gcactgtcgc cgcgttctat caccaggaca
    21961 tccagcccgg ctcgtgccat gaccagtgcg gcaacgctac cagcaacgcc cgcaccgacc
    22021 acaatggcat caaatttgtc atccgacatg cgttatgctc ccttcccgct gcgtttacag
    22081 cgggagatgt ggttgaaagg ggaattagcg gctcaactgg ctaatcaggg cagggacgac
    22141 tttgtagata tcgcccacca gaccgtagtc ggcatagttg aagattggcg catttttatc
    22201 tttattgatg gcgacaatca cttttgcgcc gttgccgcca accatatgtt ggatctgccc
    22261 ggagatcccc agcgtcaggt agagatcaga tttcagcaac acgccggaga caccgatata
    22321 acgttcacgc tccatccagt tctcgccttc ggcaattgga cgtgaacagc cgacttcagc
    22381 attcagcacc gccgccagtt cgtggaccat tttcaggtca tcctgcgccg caagaccacg
    22441 accgacgcca accacacgtt ttgctttgct caggtccacg ctgcttgcgg ctttcgcacg
    22501 gcgttcgcga cagagaattt catgacgcgg agcaacataa ggtaccgttt ctgtcgggca
    22561 ctgatgagag gtatcactgg tgcacggttc ctgaacaccg ggtgcaaggg taatgatcgc
    22621 cagcgggctg ttgatctttt cctgagcgaa cgctaacccg ccatacatcc ggtgttcggc
    22681 gcaaatgtga ccatcgacaa tatccaccgc cgtggcatcg ttcaccagcg ccgcattcag
    22741 ttgcacactt aaccgtgctg ccagagcttt accacgtttc gtcgcggcca acagcaacat
    22801 agcggggtgt ttatctttca gcagggcagc aatgctttcg gcgtaatttt cagtgcgttg
    22861 cagcgcgtcg ttttgctcaa gaacataaat acattttgga ccataaggca taactgcctg
    22921 cgcctggtcg gtattttgta caatggcata cacctgttgg ccccattgct gagcgccgcc
    22981 aaacagttca gcataacgtt caggattatc gctaaagacc cagacgctgt ttaattgact
    23041 catcgtttct tctccgtagg cttcagttca gggctttctt cagatgttcc gccagctcgg
    23101 caatggcctc cggcgaatcg ttatcgataa tgatgtgctt acgttctgtt tgcggcggta
    23161 cgcgaatgcc gaccagttca gcaagtggcg cgctctggct ccagtcaata tcacttgcct
    23221 gccactgatt taccggtttt ttacccgcgc cgagaatggc tttcatcgaa ggaatgcgtg
    23281 gcacgttaat atcggaggtg acgcagagca cggctggaac agagagttca ataacttcaa
    23341 catcatcttc aagcgtgcgt tcaatcacca gtgtattgcc ctgacgctga atagcactca
    23401 ctgcattaat caccggaagt tgcagaattt ctccgaccag caagccaacc tgctgggcat
    23461 aaaggtcgcc ggaaccttca ccaaagatca gtaaatcgaa gccgatcttt tcaattgctg
    23521 ccgccagcgc ttttgcggta tcgagaggca gtgcatgttc aagttgcgca tcctgcacca
    23581 aatacaggct gtgcggcccg cgggatagca cgtctttgcg cactttcgag ttctgcaaca
    23641 atgagccgcc aacggtcagc gcggctatct catcgtcatc tgttgtgagc tggcttgcag
    23701 cttcaatggc attgagatcg aactggctga ttttggcgtc ggcattgtcg aaattcaggg
    23761 tgtattctgg agtgacaaca atgtcctgtt cttcaggcac cagcttaaag caggttatta
    23821 ttttcatgat ttctcctgtg agtcgcatac ggaaccatct ccgatggaag gtaaaaatgg
    23881 cctgaaaata gtgtgcgata gcggtgcggg gttttcgacg gagcaagctg ttttccttgc
    23941 caacaatctt gcaaaaaaaa tgcgatcgct aaaacaaaag tgcaatttgc ggcaatatag
    24001 ttattccatg accgtacaat cttgcattca gtgtaaataa tcttgtttag ctctcgctaa
    24061 acgcggttgt ataaccgcaa tacacgccgt attttatccg gcatatattg tttttgcttg
    24121 ctcatggaat agggaggttg tcatgtatca acgctgtttt gataacgcca gtgaaacgct
    24181 gtttgtcgcc ggtaaaacgc cacggctttc acgttttgca tttagcgatg atccaaaatg
    24241 ggagtccgga catcacgttc atgacaatga aaccgagttg atttacgtca agaaaggggt
    24301 tgcaaggttt accatcgatt cttcgttata tgtcgcacat gcggatgaca ttgtggtgat
    24361 agaacgcggt aggctgcatg cggtggcctc tgacgttaac gatccggcaa cgacgtgtac
    24421 ctgtgcgctg tacggctttc agtttcaggg ggctgaggaa aatcagctac tgcaaccaca
    24481 ttcttgtccg gtaattgccg cggggcaggg aaaagaagtc ataaaaacct tatttaatga
    24541 gctaagtgtg attttgccgc aaagtaaaaa tagccaaaca tcttcgttat gggatgcatt
    24601 tgcctatacg ttagccattc tttactacga aaattttaaa aatgcttatc gttcggagca
    24661 ggggtatatt aaaaaagatg ttctgataaa agatattctt ttctatctga ataataatta
    24721 tcgcgaaaaa atcactttag aacagttatc gaaaaaattt cgtgccagcg tcagttatat
    24781 ttgccatgaa tttaccaaag agtatcgtat ttcccctatt aactatgtta ttcaacggcg
    24841 tatgacggaa gcaaaatggt cactcactaa tactgaatta tcacaggcag agatctcctg
    24901 gcgtgtgggt tatgaaaatg tcgatcactt tgccaaactg tttttgcgcc atgtcggttg
    24961 ttcgcccagc gattaccgta gacaatttaa aaactgtttt gcggaacaag aaatcctatc
    25021 tgaatttcct caaccggtaa gtcttgttgg ataaatctgg cggcgcagtt tgttaagaag
    25081 ctgcgccgcc tgcagattat ttgttctgat agtctttcag gatctgccga cctgctacgt
    25141 aaatcataat ttcgtctgta ccgccgccga tacgttcaca acggacatca cgccagaagc
    25201 gggagacgcg cgcttcatcg gtatagccca gaccgcccat gatttgaatc gcatcatcaa
    25261 tgacttccat tgcggtacgt gcgcaataca gttttgccag cgccgcgctg gtgcgcagtg
    25321 actgatgctg atcggcttgc catgccactt tcagcaccat gttgcgcatg ttgtcgatct
    25381 taatcgccat cagcgccagt ttttcctgga tcatctggtt atgaccaatg ggcttaccaa
    25441 aagcgatacg ttggttggca tagcgggcgg catcttcaaa ggcgcattcg gcaaagccgg
    25501 tgctgcgcgc ggcgttgatc aggcgctcca tctcaaagtt gtacatcaca ttgaggaaac
    25561 ccattccttc ttcgcccacc atgtcgctct cttcaacctc cacgttgtcg agatagactt
    25621 cgcaggtgct gagcatatgc cagccgattt tatgcagcgg gttaatctta atgccgggct
    25681 tactggagtc gacccaccac agggtgaagg cttttttggg atctttcggt tgcggatcgc
    25741 gcgccaacac cagcatatac gggtactctt tcgcaccggt aataaaggtt ttttgtccgt
    25801 tgatgtagac cttgccgttt ttacgcgtgt aagtggtagt ggcactgttg ttatctgagc
    25861 cagcacctgg ttccgtcaac gccagggcat aggcgggatc acccgtttcc agggtgcttt
    25921 ctgccgtttt acgtagctgc tctgcagaac cgaaacggcg catactgtga atacattgac
    25981 cgttggtgat caaaaatgcc ggagcaccgc attttgacac ttccatcagc gccagcattt
    26041 gggtgacgta atccgcaggg ataccaccaa attcttccgg cacgccaagc atggaaatac
    26101 cgttatccgc cagcgcccgc ataaactcac gcggatatgt cccgttttga tcgcaggtgc
    26161 ggaaatactc ttccggaaag ttagtcgtaa tcagttcgcg aatactggcc agcagcagtt
    26221 cttgttcttc agttaaagaa aaatccatca ttatactcct attaatgagc cgcttcaggc
    26281 aggacaaaac ccatcgccgc atcgataaat aatctttcgt ccatcagttt gagttctggc
    26341 gaaatcactg gggtgaagtc cattttgtcg agaatatctt tttgtaaatc gacgccaggg
    26401 gcgatttcga ttaaatgcag gccgtcttct ttcagcgtga atacagcacg ttcggtgatg
    26461 taacgaacat ccagtccccg ttcgagcgcg atttttccgc taaaagtaat ttccggtagt
    26521 tcccgaataa atttatttac ccgtccttcc tggacgatat gcaatttgcc atcggcaatt
    26581 tctgttttta aactgcccgc agttaacgtg ccgcagaaaa taattttctt cgaggtggca
    26641 ctgatatcaa taaatccacc ggtacccatg attttgccat tgaatttatg cacgccgacg
    26701 ttaccgtgct ggtcgacttc agcaaaactc aaataacaaa catccagacc accaccgtga
    26761 taaaaatcaa actgggacgt catatccaga atggcgcggg tattcacgtt cgcgccaaag
    26821 gcgataccct gtgaagtaat tccgccaatc ggacctgttt ctaccgtcag aataaagtca
    26881 tcagcacaac cttcttcccg ggcgaccagg ccaataccgt cagcaatacc gacgccgaca
    26941 ttccccaccg cgcctttacg catttcgaat aatgcacgcc gcgcaactaa tttacgttgg
    27001 tttaggggca ggctaagttt ggtgctgtca tcaagggtga agtcaccaga aataaaacgg
    27061 ttaaccggcg caccgccata cagttggctt tgatccggat cgaccaccac aatatccacc
    27121 agataacccg gaatacggac agatttagga tgcagcgtgg ctttcttaac cattttctgc
    27181 acctgcatca tcacaatacc gccgttattg tgcaccgcct gggcaataac caatgcgtcg
    27241 agatacatca cctcatcttc aaaagtggcg tagccttcac tgtcgcaggt ggtagcgcga
    27301 atgaaggcga tatctggcgc aatcgctttg taatagagat attctttgtt atcaaactcg
    27361 accagtttaa tcaggtcttc tttcgtgact tcattcagtt tgccgccttg ctggcgtgga
    27421 tcgacaaatg tcccgatgcc aatatcacta ataataccag gctggtgggc tgcggcggcg
    27481 cgtaaggttt gtgtaagtac accttgtgga tagttataag caataatttt attttgttct
    27541 gcgagttcag aaatacgcgg cgattgtccc cagtgaccac ataatgccca tttcaccaga
    27601 ccttcttgcg ccagaggact aataccacgg tcggcgcgat cgccaagccc tgtcggacta
    27661 ataatcgata aattacgtgg cgtttgagtc tgtttatatt tatcagcaag agcagtaatt
    27721 aacgtggtgg cttccagaat accgccgcca gcgcctaaca cacaaagtgt tgcttcgtcg
    27781 ggaatataat tcaccgcttc ctgtgccgac aggaccggca ctcgtccatt aatacgaggt
    27841 ggttttacag gtttcattgt taattcctga ttcaggtgtg attttcccta cgccagatgg
    27901 ataattagcg cacagagact cacgaaatat ttttattaaa tacggctgaa tatatttttt
    27961 tagttcggcg ggggaggata ttcccgccga aatattattg cttatgcctg atgtaaaata
    28021 gttaatactg tgcgcaaatc atttaccgag atttgccctg gcgcagaggc tttttttacc
    28081 gcaccaaaag ttgccgccga gccaaatact tcaccagcca gacgagaaat tacgccagtt
    28141 tttgccattg acatcgtgat gattggacga tcggcatact gctcctgcat ctccagggtc
    28201 gcggcaagca acgtcagcac atcgctggta ctttgcggca tcagcgcaat cttaggaata
    28261 tcggcgtcga aggactgcat tttgcgcaga cgggcaatga tttcttcggc ttccggcgtt
    28321 ttatggaagt catggttgga catgacaact ttcacatcat gcgcgtgggc gtaggcgacg
    28381 gtttctttaa cctgatcatc gccggtaaat aactccagat cgatcatatc aaccaggccg
    28441 ctgtcgatgg ctgcacgatt gagagcaata taagcctcgg tggaaatcgc ctgctcgccg
    28501 ccttctttgg cactgcggaa ggtaaacagc agcggttttt ctggcatggt ttcacggaga
    28561 atttttgccg ccgccatgac agactccaca ttggagaggt cggcaaagtg gtccacacgc
    28621 cattccagaa tatcaaagtc cgcttcacga taggcgagag cttcggattt cacgcgggcg
    28681 atatctttcg ccatcagcga gacgatgatt ttaggtgcgc ccgtaccaat gacgagatct
    28741 tttacagtta cggttttcat tttttaccct ttctgcacgc ggtcagcctg tcaggcaccg
    28801 aaccccatga cctgtttaac atattccaga gggaaatctt tgccagtcca taacgtgaac
    28861 tgttcagccc cttgccacaa caacatgccg tatccatcaa tcgttttgca accagcttgt
    28921 tgcgcctgct gcaataactt cgtcatatgc gggttataca cgcattcagt gaccagcagt
    28981 cccggatgta acagactgat atcattaact aatgattcat tctcaagggg tttcataccc
    29041 acttttgtgc cattggttaa aatgtcggcg gaagccaggg cttcagcaaa ggcttgttga
    29101 tcggcgagat cggtaaccgt gacgacacaa tcggtgtttt cgttaacccg ctgcgcgaag
    29161 gcgagggctt tatcgaagaa ctcatcccga cggttaaaga gtttaatttc ttttaaacct
    29221 tcaattgccc cctgcgcgcc aatcgccgtt gaggcacctc cggcccctaa cagcaccatt
    29281 gttttgcctt tgatatcaaa accgctctct ttaatggcgc gaatatggcc cgtgccgtcg
    29341 gtgttatagc cgcgcaggta gccatcatca ttaacgatgg tgttgatggc acccaccagt
    29401 ttggctgctg gcgttaattc atcaacatat tcacacgcca gttgtttgtt cggcattgat
    29461 acaccagttc cgcgcatttt tagagctttt aatccttcaa ttgctccggg aaagctatcg
    29521 ttatccactt cgaaggccat ataggtaaat ggcaatcccg ctttttctaa ggctttattc
    29581 tgcatttcag gcgataaact gtggcggata ggataagcca tcaacccaat caattcgtat
    29641 tttgcggtaa catccataat taactccttt aacctctatg cttaattgtg tttagccggg
    29701 tgctaaattt acgctcgcca aaccgcacat cattctcagg aataatgaaa acgcggtaat
    29761 agcggataaa gacaataatt gcggtaatca gggccagcag cgcgaaagta aaatcgagaa
    29821 caatgatata ttgcaggccg atgttcgaca gataaccggt aatcagcgga ataacaaaat
    29881 tagccaatcc acccatcatc atataaatac tggtgacttt ggctttgctt ttggggaaaa
    29941 actctgacat gaccgaaacg ccgagctgta aaatgccacc agctgctgag aaaccgataa
    30001 caaaggctcc ggcattacac accagtggag aagggtacag gtagataatg gctgctgtta
    30061 ttgttgccag tgcagagtta aatacattag cccagatggg ccggaccatt tttttcagta
    30121 gtgcggcaaa aataaagaca cagaccaacg agcccatact gtaataagag atggttttta
    30181 atgcctcagc ttctgacata ccagcaaaag ccatcgcata tttgggcatc cacaccacaa
    30241 tcacataaaa ggtcgagaat gcggctacac cgaacagtac cgatgaaaca ccttccagcc
    30301 agactaacgg tttgctgttc atttgcggta attcattagc tacgctggcg tccaccaact
    30361 ggctggggaa tttgcttttc aacagcatca gcgtgatcag tacaaatagg atgcctggaa
    30421 taatcagccc atagccgtac cagatgttat tgagcaacat atagctcacc agcattgggt
    30481 agaacatttg tccgaatgac accatcgctt taaccagtat gaccgccgaa ccagaggctt
    30541 tcggaaagca ttccatgagc gcggggtagc cacccgtatc cagcgctgag ttagcgatac
    30601 ctacgcacac tgccagaccg taggcgagag ttaaattcgg gcaagcggga ataccaaaga
    30661 agaatagcag atacattatt actgccatta atatcaccgc ccgacgacca aacttatcgg
    30721 agatcacacc gaagaataaa atactgatca atcgccccaa accaataccg gaaattaagt
    30781 aggcaatgcc cgcgttgtca gtggaaaact tttccgccag agatgacatg ttttgggcaa
    30841 gcgtaataac actaatgccg tgcaggaagt agctgaagta aatacaaaga acagccagga
    30901 taaatggcgt gctgaaagcc ttattttgag acattttcac agctccttgc tgaaagataa
    30961 tttcgttacc gtacgtattg ttcttcgacg gtttacataa ttactatact gaagatgatt
    31021 tttcctgggt acatggcata aagtttatgt ttccaggaat attgcatgtc atagtttgtt
    31081 tttgatcgcg attttcctta gttagcgcag aaagagttat ttatccgtaa gatcttgcat
    31141 gacctgaaaa gaatattgca ttacccaccc ggagcgacat tttcctttag cgagtgatgc
    31201 cgcgtttttt tgcggctgcg taagccgata aacagtgcca gtaaaaaacc gatggcagcg
    31261 atggcggtat cgaaccacat aatatcggca atacttcttt gggacagatg agccgtaatc
    31321 aacggaatag taaaggtcgc aatactgccc gcactgtaat agatccctgt agctttacct
    31381 ttagcgtaag ggaaacgttc agccattaac gtcaggccaa tttgcacaac acctccagca
    31441 gaggtaaaac caatgacaaa agcaaatatt atcaccacat aaaatgtggg atgcaggcag
    31501 acggtaaaca gagcaataaa tgagataaag gtgtacagca tcagtaatgt tgttgggcga
    31561 acggtattac gaatgagtgg agcggtaata aatacacaaa gcagcgaacc cacggtgtag
    31621 atactgagta gtttaatcga catagtgtat gacatgcctg caacaaattg tccgtactgt
    31681 gccagccact ggctaaccag ataaaacgtt gccattgaga tatagccata taaggtataa
    31741 ctggctaaat cgataattga acagcgatgt tccgtagagc tggtggtttt ctttatgaca
    31801 ggtaagcgat gacccggatg gggtgggaac gtacaaaggt ataaaaacag agcgttaata
    31861 aacataatgc ctgcagcaat cataaaggac caaccgaacc acagttcagc ccacactaac
    31921 aggctaatga ttagcggtaa taaaaattgt ccgctggaaa caaatgcttt aattaaaata
    31981 ttggctgtcc caggtgagcg tggaaaagct tccattaaac tgggataagt gcctgcatcg
    32041 agaaaactgt ttgccattcc tgccagaaag ccaaaaacat aagcgataat gatgttattg
    32101 gtgtgcagga tgccaaaaaa gaaggccata tagcagcaca tcccgagcat gataaaaggg
    32161 cggcgaccaa agcgatcgga taataatcct gcaaaaagca agacacttaa tcgaccaatg
    32221 cccagcgatg agataactat cgagacaccc gcggcattag tctgccaaag tgtctccagc
    32281 gaggccatat tcaggctcat caaaatgacg cccataccat gcaccaggta attaaaatac
    32341 aacccaagtg cggtagggta atagggattt ttcataggtt ctacttagca aaggtgcttt
    32401 aaaaagtgta gcgatgttgt gtcagtatcc gttcattatt gttgtaacaa cgacaattcc
    32461 tttttgtgga gaaattaaca aagccgctcc agatcgtgtg catataattc agcggctacc
    32521 gattgataga tcttccagtc aataaaattg ccatcagggt agacttgttg aaacgcggtt
    32581 aagtcgggga aaaaacgcat ggtgttgttg ccgatgcggt tatttatttt ctctatgatg
    32641 gcatgaagat gtttttttcg ttgctgacgc atagccaaca gttgctcgac tacacgatca
    32701 ggaatggcgc atttgccatt ttcccactga cgccagcttt cactgtcacc ggtctgggca
    32761 atccaggtcg cgcattcatc aatagtcatg gcaaaaatat ggcgaagtgc ctggagttca
    32821 taagcgttca tcgtgtaatg gctgcatgaa ataaacaata aaatataaat aacattatca
    32881 gcctgctgac ggcttgaaga gagtcacagt atcttgtgca acattattgg aaagagtgag
    32941 gggcgaggta gcgaagcgga aaaatgttaa aaaaaagccc atcgtgggag atgggcaaag
    33001 actacacaca gcaattcgtt gtttcactca ggggatttcc atgcttataa atcaatatgt
    33061 tgatttataa ccgtaagcta atagtaggca tacggacttt ttgcgtcgat cagattcgtc
    33121 tcattagttt gaaaactgta aagaatttgc gacaggtact accagctgaa tggcggtaag
    33181 caggaaaata tggggaagag caagcaaata tcaattagcg ggggttaaca atcaggcggc
    33241 agagccgcct gatgagaaat tacttattcg gtttctcgat ttcgccaagt tcttcgagaa
    33301 tttcttccgg ttggtccgtc ggcggcggga cttcttccac ccacgcggca aacagacgcc
    33361 aggaaacggc taacagaacc ggaccaataa acagaccgat catcccgaaa gcaatcaaac
    33421 caccaataac accagagagg atcaggatca gcggtaaatc ggcacccatg cgaattaaca
    33481 ttgggcggat gacgttatcc agcgtgccaa ccacaccgct ccacactaac aataccgttc
    33541 cccaggtggt atcgccagtc cagtagagcc agataatcgc cggaatcagt accggcaacg
    33601 ggccaagctg gacaaggcag gagaggatca ttaacaccgt tagcaacgtt gcataaggta
    33661 cgccggatac ggcgaggccg ataccgccaa gcactgcctg tactaacgcc gtcaccacca
    33721 cacccagcgc caccgcgcgg atagcctgtg ccgccagcag tacggcggca tctccgcgaa
    33781 cgcctgccag acgggttgca aaatggcgaa tgccttgtgc cacctgttca ccgcgccagt
    33841 acagcagggc actgaagaga agcatcagcg cacaatgcac cataaagcgc ccgatatgcg
    33901 ccgcctgccc aacgaaccag gtggtggtgg tgccaatata agggcggact ttagccatga
    33961 tcgccgtccc ccccatatcc agcaagttgt gccagcctgc atacagcttc gcgccaatca
    34021 ccggaatggt attaagccac gccagatcgg gtaacgtcat gtcgccgctg gaaatggctt
    34081 taattagcgg gccgctgccg tcgacgatac tgttaaccag caaagcaata gggatgataa
    34141 acaccatcac taataacagc gtcatcacca gaacggcgag agagcggcgg ccaaacatga
    34201 tcttttgcaa acgtaacaat accggccagg tggcgataac caccgtaccg gcccatgcaa
    34261 agccgagaat aaagggttga acaatccaca gacatgccac aatcatgatg gctaaaaaca
    34321 gcaccgaaag cagaatttgt gcgacatccc tgggctgacg aacatttacc ataactactt
    34381 ttcacctttt ttgtgcgtca aatcgtcgac gcgacgtgaa cacgcgaaac tcacctgcat
    34441 aatgatgagc aatttcagcg gtttttaaca ggccgattct gcccttaatt ctgatgggcg
    34501 tgttagaaaa aaatgtggta caacaactca ggcaacacgc aaacgattta ctcgtcgtat
    34561 ttcaagttgc atgtgctgcg gctacgttcg ttcaccccag tcacttacta ttgtaagctc
    34621 ctggagttca ctcgcttgcc gccttcctgc aactcgaaat ccatagagta aataaacatc
    34681 acctttcagt acgctattgc agccgcctga aagaatagtg tatcgaccta catcacagac
    34741 cgcaggaaag ggtcaatata atgattccac agatttccca ggcacccggc gtcgttcaac
    34801 tggtgcttaa ttttttgcaa gagctggagc aacaaggttt taccggcgat acggcgacaa
    34861 gttatgccga tcgtctgaca atgtcgaccg acaacagtat ttaccaactt ctccccgatg
    34921 cggtggtatt tccgcgttca accgcagatg tggcgctgat cgcccgtctt gccgcgcagg
    34981 aacgctattc atcgctgatc tttacccccc gcggcggcgg caccggcacc aacggtcagg
    35041 cgctcaacca ggggattatt gttgatatgt cccgccatat gaaccgcatc atcgaaatta
    35101 accctgaaga gggctgggtg cgcgttgagg ccggggtgat aaaagatcaa ctcaatcagt
    35161 acctgaaacc gttcggctac ttttttgcgc cggaactttc gaccagcaac cgggcaacgc
    35221 tcggtgggat gatcaatacc gatgcttctg gtcagggatc gctggtctat ggcaaaacgt
    35281 cggatcacgt acttggcgta cgtgcggtgt tgttgggggg cgatattctc gatacgcaac
    35341 ctttacccgt cgaactggcg gaaacgctgg gtaaatccaa taccacaatc gggcgaattt
    35401 ataacacggt ttatcaacgt tgccgtcagc aacgccagtt aatcatcgac aacttcccca
    35461 aacttaaccg ctttcttacc ggttacgatc tgcgtcatgt ctttaacgat gagatgaccg
    35521 agttcgacct gacgcgcatt ctgacgggtt cagaagggac gctggccttt attaccgaag
    35581 cgcggctgga tattacgccc ttgcctaaag tgcgccgtct ggtgaacgtc aaatatgact
    35641 cttttgactc cgcgctgcgt aacgcgccgt ttatggttga ggcgcgggcg ctttcggtag
    35701 agacggtgga ctcaaaagtg ctgaatctgg cgcgggaaga tattgtctgg cattccgtca
    35761 gcgagttgat taccgatgtg cctgacaaag agatgctcgg gctgaacatt gtggagtttg
    35821 ctggtgatga tgaggcgctg attgacgagc gagttaatgc tctctgtgtg cggcttgatg
    35881 agctgattgc cagccaacag gcaggtgtga ttggctggca ggtgtgccgc gagctggcgg
    35941 gtgttgaacg tatctatgcg atgcgtaaaa aagccgttgg tctgcttggc aatgccaaag
    36001 gtgccgctaa gccaattccg tttgctgagg atacctgcgt accgccggaa catctggccg
    36061 attatattgc tgaatttcgc gcgctgctcg acagccacgg cttaagctac ggtatgttcg
    36121 gtcacgtcga cgcaggtgtc ttgcacgtcc gtccagcgct ggatatgtgc gatcctcaac
    36181 aagagatttt gatgaagcaa atctctgatg acgtagtggc gctgactgcg aaatacggtg
    36241 gtttgttgtg gggcgagcac ggcaaaggtt ttcgcgctga atacagcccg gcgtttttcg
    36301 gtgaggaact ttttgcagaa ctgcgcaaag tgaaagcggc atttgacccg cataaccgac
    36361 tcaacccagg gaagatttgc ccgccagaag gtctcgatgc gccgatgatg aaagtggacg
    36421 cggtgaagcg cggtacattc gatcggcaga tccccattgc ggtacgccag cagtggcgcg
    36481 gtgcgatgga gtgtaacggc aacggtttat gcttcaactt tgatgcccgt agtccgatgt
    36541 gtccgtcgat gaagatcacc cagaaccgga ttcattcacc gaaagggcgc gcaacgctgg
    36601 tgcgtgaatg gctgcgtttg ttggcggatc gcggcgttga tccactcaaa ctggaacaag
    36661 aactgcctga atccggcgtc agtttgcgga cgttaattgc ccgcacgcgc aatagctggc
    36721 atgcgaataa aggcgaatat gacttctcac acgaagtcaa agaggcgatg tcgggctgtc
    36781 tggcctgtaa agcgtgttcg acccagtgcc ccatcaaaat tgatgtgccg gagtttcgct
    36841 ctcgttttct gcagctctat cacacccgtt atttacgccc gctgcgcgac cacctcgtcg
    36901 ctacggtcga gagctacgcg ccgctgatgg cacgcgcgcc gaaaaccttt aacttcttca
    36961 ttaaccagcc gctggtgcgc aaactctcgg aaaaacatat cggcatggtt gatttgccgc
    37021 tgctgtcggt cccctcgcta caacaacaaa tggtggggca tcgctcggca aacatgacgc
    37081 tggaacagct tgaagctctc aatgcagagc agaaagcgcg cacagtgttg gtggtgcagg
    37141 acccctttac cagctattac gatgcgcaag tggtggcgga ttttgtccgt ctggtcgaaa
    37201 aattaggttt ccagcctgtg ttactgccat tttcgccaaa tggcaaagcc cagcatatta
    37261 aaggttttct taatcgtttt gcgaagacgg cgaaaaagac ggcggatttc ctcaaccgta
    37321 tggcgaagct gggtatgcca atggtgggcg tcgatccggc gctggtactt tgttatcgcg
    37381 atgaatataa actggcgctg ggcgaggaac gtggcgagtt taacgtctta ctggcgaatg
    37441 aatggctggc aagcgcactt gagtcacagc cagtggctac agtcagcggt gaatcatggt
    37501 atttctttgg tcactgtacc gaagttaccg ccttgccggg tgcgccagca caatgggccg
    37561 cgatatttgc ccgttttggc gcgaaactgg aaaatgtcag cgtgggttgc tgcggcatgg
    37621 cagggactta cggacatgaa gcgaaaaacc ataaaaattc gctcgggatc tatgagttat
    37681 cctggcatca ggctatgcag cgactgccgc gtaaccgctg tctggcgacc ggatattcct
    37741 gccgtagcca ggtaaaacgg gttgaaggca cgggggtacg ccatcctgtg caggctttac
    37801 tggagattat taaatgatat ggaaacggaa aatcaccctg gaagcactga atgctatggg
    37861 tgaaggaaac atggtggggt tcctggatat tcgctttgaa catattggtg atgacaccct
    37921 tgaagcgaca atgccagtag actcgcggac aaagcagcct ttcgggttgc tgcatggagg
    37981 agcatccgtg gtactggccg aaagtatcgg ttccgttgcc ggttatttat gtaccgaagg
    38041 tgagcaaaaa gtggttggtc tggaaatcaa tgctaaccac gtccgctcgg cacgagaagg
    38101 gcgggtgcgc ggggtatgca aaccgttgca tctcggttcg cgtcaccagg tctggcagat
    38161 tgaaatcttc gatgagaaag ggcgtttgtg ctgttcgtca cgattgacga ccgccatttt
    38221 gtgacgttgt caccagaaaa gtgtgacgca tattgccttt ggtcattatc tgcacagtaa
    38281 acggtggtat actgtgcagg tcttgtctaa aagcgaggat gctatgtcta ctcaacttga
    38341 tcccacccag ctggccattg aatttttacg ccgtgaccag agcaatcttt cacctgccca
    38401 atatctcaaa cgtctgaaac aactagaact tgaatttgct gacctgctta ctctctcttc
    38461 tgctgaatta aaagaagaaa tctactttgc ctggcgtctg ggcgtgcatt gatcctgact
    38521 taatgccgca agttcgccaa atcgacctgg attaacctcc gtccaggttc gatgaccttc
    38581 ttaatactgc ttatctaccg cctgatattg ttcaaaaata atactaatcg cagtttgtgt
    38641 taaaacggcg ggttagcttt atgagattat tcttatcgcc ccttcaagag ctaagccact
    38701 gagagtgccg gagataagcg ccggatgggg tagaaaccct taagcctgtg ttgcacagac
    38761 ttaagggttt tcttatttct gaacgctgca c
//
LOCUS       DBANUU010000031        38459 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000031 DBANUU010000000
VERSION     DBANUU010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.031"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            153..2396
                     /gene="pdeF"
                     /locus_tag="ACIE68_001544"
     CDS             153..2396
                     /gene="pdeF"
                     /locus_tag="ACIE68_001544"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFX5907880.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGVLLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2435..3976)
                     /gene="ppx"
                     /locus_tag="ACIE68_001545"
     CDS             complement(2435..3976)
                     /gene="ppx"
                     /locus_tag="ACIE68_001545"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFX5907881.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3981..6047)
                     /gene="ppk1"
                     /locus_tag="ACIE68_001546"
     CDS             complement(3981..6047)
                     /gene="ppk1"
                     /locus_tag="ACIE68_001546"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFX5907882.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6218..6856)
                     /gene="purN"
                     /locus_tag="ACIE68_001547"
     CDS             complement(6218..6856)
                     /gene="purN"
                     /locus_tag="ACIE68_001547"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFX5907883.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6856..7893)
                     /gene="purM"
                     /locus_tag="ACIE68_001548"
     CDS             complement(6856..7893)
                     /gene="purM"
                     /locus_tag="ACIE68_001548"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFX5907884.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8218..8844
                     /gene="upp"
                     /locus_tag="ACIE68_001549"
     CDS             8218..8844
                     /gene="upp"
                     /locus_tag="ACIE68_001549"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFX5907885.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8930..10219
                     /gene="uraA"
                     /locus_tag="ACIE68_001550"
     CDS             8930..10219
                     /gene="uraA"
                     /locus_tag="ACIE68_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFX5907886.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10314..11015
                     /gene="hda"
                     /locus_tag="ACIE68_001551"
     CDS             10314..11015
                     /gene="hda"
                     /locus_tag="ACIE68_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFX5907887.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11153..11512)
                     /gene="arsC"
                     /locus_tag="ACIE68_001552"
     CDS             complement(11153..11512)
                     /gene="arsC"
                     /locus_tag="ACIE68_001552"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFX5907888.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVESEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11533..12996)
                     /gene="bepA"
                     /locus_tag="ACIE68_001553"
     CDS             complement(11533..12996)
                     /gene="bepA"
                     /locus_tag="ACIE68_001553"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFX5907889.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13209..14270
                     /gene="yfgO"
                     /locus_tag="ACIE68_001554"
     CDS             13209..14270
                     /gene="yfgO"
                     /locus_tag="ACIE68_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFX5907890.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14308..15156)
                     /gene="focB"
                     /locus_tag="ACIE68_001555"
     CDS             complement(14308..15156)
                     /gene="focB"
                     /locus_tag="ACIE68_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFX5907891.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHYIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(15178..17190)
                     /gene="hyfR"
                     /locus_tag="ACIE68_001556"
     CDS             complement(15178..17190)
                     /gene="hyfR"
                     /locus_tag="ACIE68_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFX5907892.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNVYYRYHDDGSDVTATAEYQGEMIDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17220..17633)
                     /gene="hyfJ"
                     /locus_tag="ACIE68_001557"
     CDS             complement(17220..17633)
                     /gene="hyfJ"
                     /locus_tag="ACIE68_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFX5907893.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQAPAIYLMARKIA"
     gene            complement(17626..18383)
                     /gene="hyfI"
                     /locus_tag="ACIE68_001558"
                     /pseudo
     CDS             complement(17626..18383)
                     /gene="hyfI"
                     /locus_tag="ACIE68_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(18380..18925)
                     /gene="hyfH"
                     /locus_tag="ACIE68_001559"
     CDS             complement(18380..18925)
                     /gene="hyfH"
                     /locus_tag="ACIE68_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFX5907894.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAQQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(18935..20602)
                     /gene="hyfG"
                     /locus_tag="ACIE68_001560"
     CDS             complement(18935..20602)
                     /gene="hyfG"
                     /locus_tag="ACIE68_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFX5907895.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(20592..22178)
                     /gene="hyfF"
                     /locus_tag="ACIE68_001561"
     CDS             complement(20592..22178)
                     /gene="hyfF"
                     /locus_tag="ACIE68_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFX5907896.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            complement(22183..22833)
                     /gene="hyfE"
                     /locus_tag="ACIE68_001562"
     CDS             complement(22183..22833)
                     /gene="hyfE"
                     /locus_tag="ACIE68_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFX5907897.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22845..24074)
                     /locus_tag="ACIE68_001563"
                     /pseudo
     CDS             complement(22845..24074)
                     /locus_tag="ACIE68_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(24091..25035)
                     /gene="hyfC"
                     /locus_tag="ACIE68_001564"
     CDS             complement(24091..25035)
                     /gene="hyfC"
                     /locus_tag="ACIE68_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFX5907898.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLLTPLFTGISRQIRARMHSRRGP
                     GIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGGG
                     DLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIAG
                     STHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTEY
                     SGAGLALAKWGLGLKQVVMAALFVALFLLFGRAQELSLACLLTSLVVTLLKVLLIFVL
                     ASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25046..27063)
                     /gene="hyfB"
                     /locus_tag="ACIE68_001565"
                     /pseudo
     CDS             complement(25046..27063)
                     /gene="hyfB"
                     /locus_tag="ACIE68_001565"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(27063..27680)
                     /gene="hyfA"
                     /locus_tag="ACIE68_001566"
     CDS             complement(27063..27680)
                     /gene="hyfA"
                     /locus_tag="ACIE68_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFX5907899.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVLPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(27933..28403)
                     /gene="bcp"
                     /locus_tag="ACIE68_001567"
     CDS             complement(27933..28403)
                     /gene="bcp"
                     /locus_tag="ACIE68_001567"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFX5907900.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28403..28975)
                     /gene="gcvR"
                     /locus_tag="ACIE68_001568"
     CDS             complement(28403..28975)
                     /gene="gcvR"
                     /locus_tag="ACIE68_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFX5907901.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29121..29999
                     /gene="dapA"
                     /locus_tag="ACIE68_001569"
     CDS             29121..29999
                     /gene="dapA"
                     /locus_tag="ACIE68_001569"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFX5907902.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30016..31050
                     /gene="bamC"
                     /locus_tag="ACIE68_001570"
     CDS             30016..31050
                     /gene="bamC"
                     /locus_tag="ACIE68_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFX5907903.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31263..31976
                     /gene="purC"
                     /locus_tag="ACIE68_001571"
     CDS             31263..31976
                     /gene="purC"
                     /locus_tag="ACIE68_001571"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFX5907904.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32130..32357
                     /gene="vapB"
                     /locus_tag="ACIE68_001572"
     CDS             32130..32357
                     /gene="vapB"
                     /locus_tag="ACIE68_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFX5907905.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            32357..32755
                     /locus_tag="ACIE68_001573"
     CDS             32357..32755
                     /locus_tag="ACIE68_001573"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFX5907906.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            32902..33765
                     /gene="ypfJ"
                     /locus_tag="ACIE68_001574"
     CDS             32902..33765
                     /gene="ypfJ"
                     /locus_tag="ACIE68_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFX5907907.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33780..35795
                     /gene="tmcA"
                     /locus_tag="ACIE68_001575"
     CDS             33780..35795
                     /gene="tmcA"
                     /locus_tag="ACIE68_001575"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFX5907908.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35869..36567
                     /gene="ypfH"
                     /locus_tag="ACIE68_001576"
     CDS             35869..36567
                     /gene="ypfH"
                     /locus_tag="ACIE68_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFX5907909.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36677..36877)
                     /gene="ypfN"
                     /locus_tag="ACIE68_001577"
     CDS             complement(36677..36877)
                     /gene="ypfN"
                     /locus_tag="ACIE68_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFX5907910.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36905..38032)
                     /gene="dapE"
                     /locus_tag="ACIE68_001578"
     CDS             complement(36905..38032)
                     /gene="dapE"
                     /locus_tag="ACIE68_001578"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFX5907911.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38036..38392)
                     /gene="yffB"
                     /locus_tag="ACIE68_001579"
     CDS             complement(38036..38392)
                     /gene="yffB"
                     /locus_tag="ACIE68_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFX5907912.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9350 a         9904 c        10117 g         9088 t
ORIGIN      
        1 ggttattttg tttgatatat atcaattgga actttcatat gacacttaga atcaatgctc
       61 tctatagtga tgaataatca tcattcgaag tcaggtggga tgcctgtctg aatacacctc
      121 cttcaggatg tgggggattc tgctgagcat ctatgaaact gaatgcaact tatataaaaa
      181 tacgtgataa atggtggggg cttccgctgt tcctgccttc tttaatcttg cccattttcg
      241 cccacattaa tactttcgcg catatttctt ccggtgaggt ttttctcttt tatctgcctc
      301 tggcactgat gatcagcatg atgatgtttt tcagctgggc ggcattgcca gggatcgcct
      361 tagggatttt tgtccgcaaa tatgcagagc tgggttttta cgaaacgcta tcattaacgg
      421 ctaattttat tatcattatc attctctgtt ggggcggtta cagggtcttt actccccggc
      481 gtaacaacgt ttcacatggt gatacccgtt taatttccca gcgtatattc tggcagattg
      541 tgtttcctgc aacgctgttt ctgatacttt tccagtttgc tgcatttgta ggattactgg
      601 cgagcagaga aaatctggtc ggtgtcatgc cttttaacct cgggacttta atcaattatc
      661 aggccttact ggtgggtaat ctgatcggtg tcccgctgtg ctacttcatc attcgggtag
      721 tgcgaaatcc attttattta cgtagctatt attcgcaatt aaaacagcag gttgatgcca
      781 aagtcaccaa aaaagagttc gcgctctggc tactggcatt aggtgtttta ttgttgctgt
      841 tatgcatgcc gttaaatgaa aaaagcacaa tttttagcac caattatacc ttgtcattat
      901 tgctgcccct gatgatgtgg ggagcgatgc gctatggtta taagctgatt tcgctgctct
      961 gggcggtcgt gttgatgatc agcatccaca gctatcaaaa ttacattccc atttatcctg
     1021 gctataccac gcagctgacc ataacctcct ccagttatct ggtattctct tttattgtca
     1081 attatatggc tgtactggca actcgtcagc gagcggtagt cagacgcatt cagcggcttg
     1141 cgtatgtgga cccggtggtt catctgccaa atgttcgcgc cctgaatcgc gcgttgcgtg
     1201 atgccccctg gtctgcgctt tgttatttac gcatccctgg catggaaatg ctggttaaga
     1261 actatggcat catgctgcgg attcaataca agcaaaaact ttctcactgg ctgtcaccct
     1321 tgctggaacc gggtgaagat gtttatcagc tttcgggtaa cgatctcgcg ctgcggctga
     1381 atacagaatc gcaccaggag cgcattaccg cactggatag ccatctcaag caatttcgtt
     1441 tcttttggga tggcatgccg atgcaaccgc agattggcgt cagctactgc tatgtgcgct
     1501 cgccagtgaa tcatatctac ctgctgctgg gagagctaaa tacggtcgcc gaactttcca
     1561 tcgtgaccaa cgccccggaa aatatgcagc gtcgcggggc aatgtatttg caacgcgaat
     1621 tgaaagataa agtcgcgatg atgaatcgac tacagcaggc gctggaacac aaccattttt
     1681 tcctgatggc ccagccgatt accggtatgc gtggtgatgt ttaccatgaa attcttctgc
     1741 gcatgaaagg tgagaatgat gaactgatcg gccccgatag cttcttgccg gtcgcgcacg
     1801 aatttggttt atcgtcgagt atcgacatgt gggtcattga gcatacgctg caatttatgg
     1861 ctgaaaacag agcgaagatg cccgctcacc gttttgctat taatctgtct ccaacctcgg
     1921 tatgtcaggc tcgttttcct gttgaagtca gtcagttgct ggctaaatat cagattgaag
     1981 cgtggcaact tatttttgaa gtcaccgaaa gtaatgctct gaccaatgtt aagcaggcgc
     2041 aaatcacctt gcagcatctt caggaattag gctgccagat tgcgattgat gatttcggca
     2101 ccggctacgc cagctatgca cggcttaaaa atgtgaatgc cgatctgctt aaaattgacg
     2161 gcagttttat ccgcaatatt gtgtcaaata gtctggatta tcagatagtg gcatcgattt
     2221 gccacctggc gcgaatgaag aaaatgcggg tagtggcaga gtacgttgaa aacgaagaga
     2281 tccgcgaggc ggtgctctct ttggggatcg attatatgca gggttatctt attggtaagc
     2341 cgcagccgtt aattgatacg ttgaatgaaa tcgaacccat tcgcgaaagt gcctgaataa
     2401 tgcgggccga catttctcgt cggcccgcaa agcattaagc ggcgatttca ggtgtacttt
     2461 cttcttcaat tttcaaccgc cagccagcca cgccttccca gtattcttgc tccttttcca
     2521 gatcaagcag taccagcgca ttctgactaa accagtcatg cgggaaacgc agtgtccagt
     2581 gactgtcatc agtaatcagt gtcaatgttg gcggtgtggt ggttgcctga cgttgattgt
     2641 ttagtaatac gccaaggcgc aatagctgta tcagtggcag gaactgtttc ttcttaaaca
     2701 aggtaaagcg cggcagatcg tcgagcttaa tcgctttacg gtgatagcgc accagtgtcg
     2761 ccatcatcag ctgctgttcc tgattaaaac ccggcaagtc actgttttgc agaatataag
     2821 cggagtggcg atgcaaaccg ctgtggttga tattcaaccc gacctcatgc agcatggcgg
     2881 cccatcgcag tagcgcctcc agttgcggat gcgccagctt cggttgctgt tcccgccact
     2941 gttcgtacat ttgcatagtg gtatccagca cccgtcgggc ctgctcgctg tcgatgtgat
     3001 actggttggc gaggctgctg gcggtgcgac tacgcacatc ctgatgacgg aaacgtcctt
     3061 ccatttcata cagtacgcct tcgcgtaaag ccccgtcaga aaggcgcagt tcacggatgg
     3121 ctaaagcatc aaacacaccg cataaaatcg ccagtccagg aacgaagact gttttccgct
     3181 cttcggaaag acccggtaaa ctcagcgatg cgaaattacg gtgacgtaaa acttctttta
     3241 ccagtttttc cagacgttcc ggggtaatta tcccgtcttt ctcgcccatt tccattaaca
     3301 cttcatgggc ggcttttatg gtaccggaag cgcccattgc cacgttccag ccctgaatac
     3361 ggaattgcca ggttaaagtt tccagttttt gtgctgccgc catacgagcg cgctgaaaat
     3421 tctctttatt gatgaccccg ccagggaagt aaagctgggc aaagctgaca caacccatcc
     3481 ggcggctttc aacgagaata ggttcgaaat tttcaccaat caccagttcc gtagatccgc
     3541 cgccaatatc aataaccagt ttgcgacctt tctccggttg ggtatgttcc acgcccataa
     3601 aaatcagacg ggcttcttca ttaccggaaa taatttcaat cgggtaggga atgacctttt
     3661 ccgcgcgttt cagaaagtca gtggcgttca gcgcctgacg cagcgtatgg gtgccgacta
     3721 tacagacgct ggcaggagaa aacccttgta gccgttcggc aaacagcgac agacagttca
     3781 aaccgcgcgt cattgcctct tcactcaaca tattatctgg ccccaggccg tccgccagat
     3841 gcacccgctg tttcaggcgg ccaataatct gcatggcacc atctaccaca cgggctatga
     3901 ccatgtgaaa actgtttgaa ccaagatcga ccgcagcaaa ctcctgcgga cgaggggatt
     3961 tatcgtgtat tggcataggg ttattcaggt tgttcgagtg atttgatgta gtcgtaaatc
     4021 gccaactgcg cccgtacttt gcggcgattg ccgcggggaa cgtagcgatt actgagttct
     4081 ttatcgatat aacgtgcttt gaccgtatcg ctgaacaata tgtcgatgat gtccagcacc
     4141 cgctgcttca ggcgcggatc gagcagcggt gtcgccactt caatacgata atcaatattg
     4201 cgcgtcatcc agtcggcgga agaaaggtag acctttttat cgccgccatt ttcaaaaata
     4261 taaacccggt catgttcaag gtaacggtca acaatactga tggcacgaat gttgtcgcta
     4321 atgccttcca gattggggat cagcgaacac attccgcgaa ccagcagatt aaccggtacg
     4381 ccggagctgg aggccgcata cagacgatca accaggcctt tatcgacaag gttatttagc
     4441 ttcagggtga taccactggg cagcccttgc tgcgcgttgg cgatttcgcg gtctaccatt
     4501 tcatataaca gacggcgaga gttttgcggt gacaccatta aataatcaaa tgtcaccggg
     4561 cggtatgggt tttcaataaa gttaaatacc cgccgtactt cgttggtgat acgcgcatct
     4621 gcggtcagca acgaatagtc agtataaaga cgcgcggttt tttcgttaaa gttcccggtc
     4681 ccgatgtgtg cgtaacggac cacttcaccg ttttctttac gtgaaatcag gaacagtttg
     4741 gcgtgaattt tcagccccgg cgcagagaag ataacgtgca cgcctgcttc ggtcaggcgc
     4801 ttcgcccagt gaatgttggc ttcttcgtcg aaacgcgcct gtaactcaac caccacggtg
     4861 actttcttac cgttatgtgc ggcgtggatc atcgagtcga tgatgcgtga atctttcgcc
     4921 acgcggtaaa tgttaatttt aatcgccagt acgctcgggt cgaacgaagc ctgacgcagc
     4981 agttccagca catgctcaaa ggtgtgataa ggataataga gcaacacatc gcgttcgcga
     5041 atggcatcaa aaccattgcg gaactgggct ttatcaaacc aaatgtggcg taaacgcggc
     5101 agtggtttgt tcaccagatt ggctttgccg acattgggga aattaataaa gtctttaaaa
     5161 ttatgataac gaccgccggg gacgatggag tcgtagcggg aaatagtcag tttttcgcgt
     5221 aacacttcaa ccagcgcatt gggcatatcg cgctgataaa caaaacgcac cggctcagca
     5281 gttaaacgct gcttgagact ggaagacatc aactccatca ggctggcttc catctcatgc
     5341 actaaatcgt attcggcatc gcgggtcatc ttcattgaat aggcattcag cgcgtcataa
     5401 tcaaagaagc ctttgaaaat atcatcaagg cagtaacgca gaatgttatc cagaagaatc
     5461 atcggcttgc gtcgacgcgg tgcttctggc ggtaaattca caaagcgcgg cactttatct
     5521 gatgggatct ccagcagcgc gtaacggatg gtatcgccac ggataatttc caccgccaga
     5581 taggtgtaat catctttcag gaactgcact aagtcagtgt caggattgat taaaatcggc
     5641 gtaatgtgct gacgcagata ctgcttaaaa taatgacgaa gccagttttg ttgattgacg
     5701 gagagctggc gttcattaat caggaagatc tggttacgcg ccatctccag cagcagctcg
     5761 ttgtagaggc cgtcgaattc ctgatcggct ttcagcaccc gggactgaat tttgcccagt
     5821 aaatggcggg aatgagagtt ggagccttgt tcttcgctaa taatgatgcg tcgcttcagt
     5881 tcagcgaagc ggactttata gaactcatca aggttattgg aatagatccc caggaaacgc
     5941 atcctttcaa tcagcgggtt agatttgtcc gccgcttcct gaagcacgcg ttcattgaac
     6001 gataaccaac tgagctcttt ttcgatgtat agcttttcct gacccattac cacttttact
     6061 ccgttttatt cacgggacac tgcctggata ttatggcgag caatattggc agatgtccaa
     6121 ctatgccaga aaagggggaa aaaaactgct ggagcttgcg atttacaggt gaattgaaaa
     6181 acgccagcgg cagagctggc gctttaacta cggggcatta ctcgtcggca gcgtagccct
     6241 gcggcggcag acgttgacca tccagccacg cggcgttttc gtgcattttc agacgaccat
     6301 cggcaaacca gctaatcacc agtggataaa tagcgtgttc ctgggtttgc acgcgggcgg
     6361 tgatgtcatc ttccgtatca ccagcaaata ccgggacttt cgcctgtaaa ataaccgggc
     6421 caccgtccag ttcatcggtg acgaaatgca ccgatgtacc gtgctcttca tcaccatttt
     6481 ccagcgcctg acgatgggtg tgtaatccgg gatatttcgg cagcagagaa gggtgaatgt
     6541 tcagcaaacg cccggcatag tgggagacaa acgccgggct gagaatgcgc ataaaaccag
     6601 ccagcacgac cacatcgggt gcgtacatgt cgatttcatg aatcaactcc cggtcatagg
     6661 cttcacgact gtcaaacgcg ctggcgatga gcgtatgcgt tgcaataccc gcctggcggg
     6721 cgcgttcaag gccgaacgcg tcggccttat tgctgaaaac tgcccgtacg gtgcctttaa
     6781 ttttgttagt tttacaggcg tcaataattg cctgtaaatt acttccgttg ccggaaataa
     6841 gcaccacaat attcattatt cgataaccac gcgttgttcg gaatcagagg ctttgatgat
     6901 accgattttc cacgcgtttt caccgttggc gtttagcagg gcgagggctt tgtccacttc
     6961 cggagcaggc agggcgataa tcatcccgac gccgcagttg aaggtgcgat acatttcatg
     7021 gcgctcaacg ttacctgccg tttgcagcca gttgaacact tccggccact gccaggaaga
     7081 ttcatcaatc actgcctgag tattatctgg caatacgcgt ggaatgtttt cccagaagcc
     7141 gccgccggtc aggtgcgcaa tggcatgcac atcgaccttt tcaatcaact ccagcactga
     7201 cttcacgtaa atgcgggtcg gtgccagcag atgatcggct aatggcttac catcaagttc
     7261 ggtggtttgc ggatcacaac cgctgacttc aagaattttg cgcaccagcg aatagccgtt
     7321 cgagtgcgga ccgctggaac cgagtgcaat cagcacatcg ccgtcgctga ctttagagcc
     7381 gtcgatgatt tctgattttt ctaccacgcc aacgcagaaa cccgcgacat cgtaatcctc
     7441 accgtgatac atccccggca tttctgccgt ttcgccaccc accagtgaac agcccgattg
     7501 cagacatcct tccgcgatac cgctgatcac cgctgaagcg gtatcaacat ccagttttcc
     7561 ggttgcgtaa tagtcgagga aaaacagcgg ctctgcacct tgcaccacca ggtcattaac
     7621 gcacatggcg accagatcaa taccaatggt gtcgtgacgt tttaagtcca ttgccagacg
     7681 cagcttggta cctacgccgt cagtgccgga aaccagcacg ggttcacgat atttttgcgg
     7741 caatgcacac agcgcaccga agccgcccag accgcccatc acttccggac gacgcgtttt
     7801 cttcactacg cctttgattc ttccaaccag agcattaccc gcgtcaatat caacaccggc
     7861 atctttgtag ctaagagagg ttttatcggt cactgcttgg gtccccacgc gttacttgcg
     7921 gtagaaaaat aaaattcggc gcaattctaa cagggaaagc aaacgtttgc gagactgctt
     7981 tacacaacct ttttgcacgt cttttgccca ggcacgcggc gaaagaagac ttgtgccagg
     8041 gtaaaggtta gttttcggat ggaataatct tctttcataa ccatttgaat ataaaataac
     8101 tttatctcaa atcgttatca ttttgactaa agtcaacgaa aagaatattg ccgccttgaa
     8161 gaaaggaggt ataatccgtc gatttttttt gtggctgccc ctcaaaggag aaagagtatg
     8221 aagatcgtgg aagtcaaaca cccactcgtc aaacacaagc tgggactgat gcgtgagcaa
     8281 gatatcagca ccaagcgctt tcgcgaactc gcttccgaag tgggtagcct gctgacttac
     8341 gaagcgaccg ccgacctcga aacggaaaaa gtaactatcg aaggctggaa cggcccggta
     8401 gaaatcgacc agatcaaagg taagaaaatt accgttgtgc caattctgcg tgcgggtctt
     8461 ggtatgatgg acggtgtgct ggaaaacgtt ccgagcgcgc gcatcagcgt tgtcggtatg
     8521 taccgtaatg aagaaacgct ggagccggta ccgtacttcc agaaactggt ttctaacatc
     8581 gatgagcgta tggcgctgat cgttgaccca atgctggcaa ccggtggttc cgttatcgcg
     8641 accatcgacc tgctgaaaaa agcgggctgc agcagcatca aagttctggt gctggtcgct
     8701 gcgccagaag gtatcgctgc gctggaaaaa gcgcacccgg acgtcgaact gtataccgca
     8761 tcgattgatc agggactgaa cgagcacgga tacattattc cgggcctcgg cgatgccggt
     8821 gacaaaatct ttggtacgaa ataaagaata aaaataatta aagccgactt taagagtcgg
     8881 cttttttttg agtaaagcgc ctataacaca taatacagag gataatacta tgacgcgccg
     8941 tgctatcggg gtgagtgaaa gaccgccact tttacagaca atcccgctta gtttgcaaca
     9001 tttgttcgcc atgtttggtg caaccgtcct ggtgcccgtc ttatttcata ttaacccggc
     9061 gactgtactg ttatttaacg gtattggaac gctgctgtat ctcttcatct gtaaagggaa
     9121 aattccggct tatcttggtt ccagctttgc ctttatttca ccggtattgt tactgttgcc
     9181 gttagggtat gaagtcgcgc tgggcggctt tattatgtgc ggcgtgctgt tctgcctggt
     9241 ttcttttatc gtgaagaaag cggggaccgg ctggctggac gtgctgtttc cacctgcggc
     9301 aatgggcgca atcgttgccg tcatcggtct ggagctggcg ggcgtagctg ccggtatggc
     9361 gggtttactc ccggctgaag ggcaaacgcc agactccaaa accatcatca tctctattac
     9421 caccctggcg gtcacggttt taggttccgt gctgtttcgt ggtttcctgg caattatccc
     9481 gattttaatt ggcgtgctgg tggggtacgc gctctctttc gcaatgggaa ttgtcgatac
     9541 cacgccgatt attaatgctc actggtttgc gctgccaacc ctctatacgc cgcgcttcga
     9601 gtggtttgcc attctgacta ttctgccagc ggcgttagtg gttattgccg aacacgtagg
     9661 gcacctggta gtaacggcta atatcgtcaa aaaagatctg ctgcgcgatc caggtctgca
     9721 ccgttcgatg tttgctaatg gcttgtcgac cgtgatttcc ggcttctttg gctctacgcc
     9781 aaataccact tacggagaaa acattggcgt gatggcgatc acccgtgttt acagtacctg
     9841 ggttatcggc ggggcggcga ttttcgctat cctgctttcc tgcgtcggta aactggctgc
     9901 cgctatccag atgatcccat tgccggtgat gggcggcgtt tcgctgctgc tttatggtgt
     9961 catcggtgct tccggtattc gtgttttgat cgaatcgaaa gtggattaca acaaagcaca
    10021 gaacctgatc ctgacttccg tgattttgat catcggcgtc agtggcgcga aggtaaacat
    10081 cggcgcggcg gagttaaaag gtatggcgct ggcgaccatc gtcggtatcg gcttaagttt
    10141 gattttcaaa ctgatcagtg tgctgcgccc tgaagaagtg gtgctggacg cagaagacgc
    10201 ggatataaca gacaaataat gtaccgaccg ggcagtgttg ctgcccggtt caaacatcat
    10261 gggattctgt ggtaaacttc tcgcgatttt gtgaaatcct ggttgaggta tctctgaaca
    10321 caccggcaca gctctctttg ccactttatc ttcctgacga cgaaaccttt gcaagtttct
    10381 ggccggggga taactcctct ttactggctg cgctgcaaaa cgtgctgcgt caggaacata
    10441 gcggttacat ctatctctgg gcacgcgaag gcgcggggcg cagccatctg ctgcacgcgg
    10501 cttgcgcgga attgtcgcag cgtggcgatg cggtgggcta tgtcccgctg gataaacgca
    10561 cctggtttgt tccggaagtg ctcgacggta tggagcattt gtcgctggtc tgtatcgaca
    10621 acattgagtg tattgcaggc gatgagttgt gggagatggc gattttcgat ctctacaatc
    10681 gaattctgga atcgggcaaa acacgattgt tgatcaccgg cgatcgtcca ccgcggcagt
    10741 tgaatctggg attaccggat ctcgcgtcgc gactcgactg ggggcagatc tacaaattgc
    10801 agccactttc tgatgaagat aagttgcagg cgctacagtt acgcgcgcgt ttgcgtggtt
    10861 ttgaactgcc ggaagatgtg gggcgtttct tgctgaagcg gctcgacaga gaaatgcgca
    10921 cgctatttat gacgttggat cagttggatc gtgcgtcgat taccgcgcaa cgtaagctga
    10981 ccattccgtt tgtgaaagaa attctgaagt tgtagataag gtgtttattg tcggatgcgg
    11041 cgcgaacgcc ttatccgaac tacgcgaagt taatccatag tattcgtagg ccggataaga
    11101 cgtggcaagc gtcgcatccg gcaatgtgct tcaactagcg atggagcaca tcttaaccca
    11161 cgatttccag tacctgctcc ggtggacgac caatccgcgc tttgccattc gcgaccacaa
    11221 tcgggcgctc cattagcttc ggattatcca ccattgcctg aatcagcgcc tcttcgctaa
    11281 gcgaactgtc agccaggttc agttctttat aaagatcctc tttctggcgc atcaattcac
    11341 gggcgctatt catccccagc attttcagca aatcgcgcag cgttgccgca tcagcgggtg
    11401 tctcaaggta gagcaccact tctgactcca cgccgttttc tttcagcagg ttcagcgttt
    11461 cccggctctt tgaacagcgt gggttatggt agatttttac ctgtttggtc attgttcttc
    11521 ctttaatgcg aattacatct tggtataagg cttaaagcgt tcctgcagct ggcgcaactg
    11581 gtcgatgcgc gcatcgtaac gcgcttgttg caggctgcct aatttcacct gcgaactggc
    11641 gctactcaac agcgaaatgg cctgatcgag tcgtccggcg agcgcataac cttctgctcg
    11701 cgcagccagc tcctgatcac ggttatttag cgcggcttcc gcctgtgcca gcaaatccca
    11761 gccgttgctg tcatctttat tattaaaggt gtagcgattc agaatattgg ccgcttcttg
    11821 tggttgaccg ccttgcagat aagcgttcgc caggttgagc tgcaacaccg gatttgtgcg
    11881 taaatcgcgg gcatttttca ggcgattgat cgcttcattg gctttgtttt gcccaagatc
    11941 gatatcagta gccagatcga gataccatgc gttgccaggt tctgccgcca gtaacggttg
    12001 cagcgtttta cgcgcctcgt cgtatttatt ggcttccatc gcctgtaaag cacgaccata
    12061 ttgcgccgct cgttgctgac gaacgtttcc tttcgcccat tcatccagca aatcactggt
    12121 gagctggtta cgtccggaat tatacatccc cagtgtgcgc gctttcgcca gatagaaatc
    12181 ttccgacgac tgcaccacca tcggtcgcat ctgattagca cggttgcggg catctgccag
    12241 acgactttcc ggcaacgggt gagtcaataa aatttccggc gggcgcgagg agtaacgcgc
    12301 ctgatcgagt aatttttcga ggaaggttgg catcgcctgc ggatcgaatc ccgagcgttg
    12361 cagcacctga ataccaatgc ggtccgcttc ctgttcattt tgctgggtga aactgatcat
    12421 cccctgacgc gttcccgcca gtgtaccggt cagcgccgcc atccccgcct gcggactggc
    12481 catcgccagt aaaatagaac ctaacgcgcc gacccaggtc agcggcgcac tgcgttgttg
    12541 atcttccatc gctcgcgcca ggtgacgttg ggtgacgtgg gagatttcgt gcgccataac
    12601 tgaagccagt tgactttcgt tatcggaata acggaacagg gcagagtgca gcaccacgtt
    12661 gccgccaaag aaagcaaagg cgttaatttc gtcgttattg atcagaaaaa aatgaaacgg
    12721 tgtcttaacc gaattggcat gcgaaaccag acgcatcccc agcgaattaa tatattgcgt
    12781 taacagcggg tcattaatta acggcgcgct gccgcgtagc tggcggacat aatagtcgcc
    12841 catctgcatt tcctgaccaa tggaaagcgt gcttcctgcg gaggttccca tatccggcaa
    12901 ggtgtctgcg ctgtcggcaa acgccggggc tacctgacca atggtcatag cagcaatgag
    12961 ggttgcaacc aggttttttt tcaactgcct gaacataacc tctatcctgt atttctgaag
    13021 atagtcattt gaccgatact gttaaatatc gttcccaccg cgccgccttt gagtgtaacg
    13081 gcgaagatta gcccactcaa atgacttttc agtttcctga atccgtcata aaaagcgaag
    13141 tcttttttgt gacatttcga tacaattgcg gatcgcaatc ccgctcttga ggttcaggga
    13201 aggtttttat gctcgaaatg ttgatgcaat ggtatcgccg ccgttttagc gacccggaag
    13261 cgattgcctt gctggttatt ttagttgccg gatttggcat tatctttttc tttagtggcc
    13321 tgcttgctcc gttgctggtg gctattgtgc tggcctattt gctggaatgg ccaaccgtgc
    13381 gcctgcaatc tattggctgc tcccgccgct gggcgacgtc gattgtattg gtggtttttg
    13441 tcggtatatt gctactgatg gcgttcgtgg tactgcctat cgcctggcaa cagggcatct
    13501 acttaatccg cgatatgccg gggatgctca ataagctttc tgactttgcc gccacgttgc
    13561 cgcgccgcta tccggcgtta atggacgcgg gcattattga tgcaatggcc gaaaatatgc
    13621 gcagtcggat gctgaccatg ggcgattcgg tggtgaaaat ttccctcgcc tcgctggtcg
    13681 gtttgctgac cattgccgtc tatctggtgc tggtgccatt gatggtcttc ttcctgctga
    13741 aagacaaaga gcagatgctg aacgccgttc gtcgggtgct gccgcgcaac cgtggactgg
    13801 caggacaggt gtggaaggag atgaatcaac aaatcaccaa ctatatccgc ggcaaagtgc
    13861 tggagatgat cgtggtgggg atcgccacct ggctggggtt cttgctcttt gggctgaact
    13921 attcgctgct gctggcggtg ctggtcggct tctcggttct tattccgtac attggcgcat
    13981 ttgtggtgac cattccggtg gttggcgtgg cgctattcca gtttggcgca ggcacggaat
    14041 tctggagctg cttcgcggtg tatctgatta ttcaggcgct ggacggcaac ctgctagtac
    14101 cggtgttgtt ctccgaagcg gttaacctgc atccgctggt gattatttta tcggtggtga
    14161 tcttcggtgg tttgtgggga ttctggggcg ttttcttcgc cattccattg gctacgctga
    14221 tcaaagccgt gatccacgcc tggcccgatg ggcaaatcgc gcaagaataa tcttcagccc
    14281 gacgaactgt ttcgccgggc tgatttttca ggattcctga cgtaaataaa tagcccgata
    14341 acaaatactc accagcactg caccgccgat aatattaccc agcatcaccg ggagcagatt
    14401 ggcggtaata aaatggctga ccgtcagtgc cggaaaattg tctgcgctac tgtgcgccag
    14461 ttgccagaag gaggtagggg cgaaatggcg aatggcaatg gcgaacggaa tcacaaacaa
    14521 attggcgata tagtgctcaa agccactggc gacaaacagg gtgatgggca aaatcatggc
    14581 gacgattttg tcgcataacg aacgcccgca ataactcatc cacagcgcca ggcaaaccat
    14641 cagattgcac ataatgccga ggctgacaga ttcagtaaat gtatgatgca ttttgccctc
    14701 ggcgcagtgt aaaaccgcga ctccccacat cgcgttttca ctcatcacca gcccggaaaa
    14761 ccagattaac aaactgaaac aggcaatacc tgccagatta ccgcaggcca ccagaagtgc
    14821 gttaatcagc caagttcgcc aactaataac gccccgactt tttgccatta ccgtcattac
    14881 cgacgaggtg aacaggttgg tgccgcaaac cgccagcaaa ataaagccaa gtgtaaagca
    14941 aaggccgccc accagatggg ttaatgcctg tgaagacggg gcatcggcga taactgaaag
    15001 gtaaaaagta aatccgatcg ccataaacac gccagcggac atcgccatta aaaagaccga
    15061 cactttactg cgggcaattt tatgggcagc aatccgttca gcgattgccg cttttctggc
    15121 gctcaactgc aagtcgaaag agagtttgtt tctcatgcat actcctgcaa aagcagatta
    15181 caacacctcg cgaaccgaga ttcccagacg ctgcattcgt gacagcagcg tggtgcgctt
    15241 catccctaat cgtgtcgccg cgccacgggg cccggcaaca atgccattgg tttctcgcaa
    15301 tacctgaaca atgcgctgac gttcttcttc gtcattctct ggcgtcgtcg ggtgcagcaa
    15361 ctgcgccatt gaactgcgaa gcgctgaatc ttcatttagc gtcggcagta aacggctttg
    15421 tcggacatta agatgtaaat tcagactgtt accgcgagtc agcagtaccg ctcgctcaat
    15481 cacgttttcc agctcgcgca cgttgcccgg ccagtcccac gacatcaact ggcgtagcgc
    15541 ctcggtcggg atggcgtcaa ttgaacgatt catatggcgc gccatttttt gcgtgaaatg
    15601 ctttgctaaa agagggatat cttccggacg gtcgcgcagc ggcggcaatt ccagtgggaa
    15661 gacattcagg cgataaaaga gatcgctgcg aaactggcga tcttcaacca tttgccacaa
    15721 atcacggttg gtggcggcaa tgactctgac atttaccggg atcgttctac tcccgccgag
    15781 acgctcaatc tcccgttcct gcaatacgcg cagcagttta ggctgaagtt ctaacggcag
    15841 atcgccaatt tcatcgagaa acaacgtgcc gccatcggca atttcaaaac gaccacgatg
    15901 ggtattaatc gcaccagtaa acgccccttt gtcatgaccg aataactcac tttccagaag
    15961 actggcgggg atggcagcgc agttgatttt gaccagcggc ttgtcgcgtc gcgggctaag
    16021 ttgatggatc gctctggcga tcacctcttt gccggttccg gtttcaccgc aaatcaacac
    16081 tgtactgtcg ctcttcgcca caatatctac ctgctggagt aggtcttcca ttgcctggct
    16141 ttgatagata atgtcaccga tgcccagatt cgaaaggagc tgctcgctaa gctggtggtt
    16201 ttcttgctgc aaactttcct gcaaatcggt catgctacgc caggcatcgg cattgccaac
    16261 ggcaatagcg atgcgatcgg ctatgtgttg tagtagctgg cagttttcct cactaaagag
    16321 agtggaagag gtatgcgcca gcaacaatgc acccggtgta tggttgccaa aggtaagcgg
    16381 tataaggagc gcagattcgc agccgttaga tgcaagtaag agtaataacg ggtcgcgttg
    16441 ccagagaaac agatcgtctg cacggtgcgt caaggtcggt tgtcgggttt gtagcgtttg
    16501 tgtcagcaat acactgtcgc caggcatatt gcgtggcaga cacgcacaat gtgaggcaga
    16561 aagatcgctg caccacaggc tgaacttctc gttctttcga tgatcgccca gtaccatact
    16621 gactgaagcc agaccgaaaa aatgatggat ctcacgagcg acgtcagcga tcagatcgtc
    16681 gagatcaaga tgtgacagca ctgcattagt gatatccacc agtacccgat gatgatcgcg
    16741 atctttctcc agcaaacgca gcgccacctg ctgttcttca tcattgagcc atgattgcag
    16801 aaccggggtg ataatgcgtg ccagccagtt aaacagtgtg caatcctggg gagtaaaacg
    16861 gcagccgtgg gtgcgaataa agcgtagctc cgccattcca ctactgccga gaaggacggc
    16921 gtgacgcgaa taatcgatca tctcgccctg atactctgct gttgccgtca catcgctgcc
    16981 atcatcatgg tagcggtaat agacgttgct tttgagaaca tgcagttcaa taagttgtac
    17041 cgggctgaac ggcggcggca gcgggatgaa ggcgcgtaat aaagacgcct taccgtgttt
    17101 ctgcgctaac cgctccgcga gcattccgtt taccgcttca attgttattc cttgcggcgg
    17161 ggcaaacatc gcctcgtctg acatagccat caacagtcct tattaatgag aaatccttct
    17221 cacgcaattt ttctggccat cagatagatg gccggtgcct gcacaatttc atccaacaga
    17281 tgaatgaatt gaatgctacg cgcctgccac acagacgttg cgtcatctgc cagtggcgcg
    17341 aatgcccggg tcaacagggc ggtgtggctg gcgtcaataa caatctcacc aaaagtcatc
    17401 acccccagca tcttacgtcg ggcttgctcc tcttcgacct gggcaagcca atcttcgtat
    17461 tccgtcagtg ggcagcggaa ggcgacattc agacaatcaa tcacgccaac gtgatggccg
    17521 atagccagcg agtaatacat tacctgagag ctgtgttccg gcatctcgtc gctactggcg
    17581 acaaactttt ttcgcagcgt ccagaaaaca atttccccgc actcttcagt catgtaaccc
    17641 ccggagtacg cgaaagagtt gctgaacgat actattgaga cgtggatcgt cggcatcgcg
    17701 caaccaggtg ttaacccgat ttcctgtagg atcgtcgctt aaatggcgca ggagccgatc
    17761 gcaaatttct cgcccctgac gatagcccgc cagccgccgc gcttctcgct caatggcgat
    17821 acgctgtgat ggcgggatct gcggccacaa cggttgcata gtcaccccgg tgggatcgcg
    17881 ataatccacc gcgtgaatct tctgttgcag caaaccgagc gccacggcga aaccgtgaat
    17941 ggtggcggcc ggtgttggcg ggcagccggg gatccaaaca tcaatgggga caatggtgtc
    18001 gctgccgccc cagacgctgt agagatcgtg gaaaatccgc cgccggcacc gcacgcgccg
    18061 taggaaacac agattttatg atcgggggca gactcatacg cccgaagtgc aggcatacgc
    18121 atcgcccggg tgactgcgcc agtaaataac aaaatatcgg cgtgacgcgg tgatgaaaca
    18181 accttaatgc caaaacgttc tgcgtcgaat actggtgtaa tggcggcaaa aatttcgatt
    18241 tcacaggcgt tgcagccacc gcaatcgacg cgataaacgt aggctgagcg acggatatcc
    18301 tgtagcaaat gccgcttcat cttttgcgtt tgctcgtcca gcgtgatggg ctggctgaca
    18361 tgttgtgtaa gcactggact catagctgct ccttagccac cagcggtaca tctgtatcgt
    18421 cgttgagcag cgtcgcgcgt tgtttgcact ccgggcagac gctcgcttgc gcccataaca
    18481 tttcgcggtt ttgtggcgca ttttgttgct gtgccaacaa ttcagcagcc agtgcgacgg
    18541 ttttttgctg ggcaaacggg cgttcgcaac ggctgcaacg ttgtagatgg aacgtcgcgc
    18601 gggtatagag atcggcttta ttggtgacgg tcagttcaaa gttattggta agctggatgg
    18661 ctctggtcgg gcacacttct tcacaacgtc cgcagtaaat acaacgcccc agatagagct
    18721 gccaggtgcg cgaattttgc tggtcgtcgg tctggatagt cagcgcattt gccggacaag
    18781 cacaggcgca ggcaccgcag gcaatacatt ggctgggcat caggtccggt ttcccgcgaa
    18841 agcctgggct gacctccagt ggcgcgaagg gatatttcac cgtcgcggtt ccggcgcgca
    18901 taatagtttt cagtaacttc agcatcttct gtccttactt cagcggcgaa cggttacgat
    18961 caatgccgta gcgttcgatc tctttatacg gcacggtttt tgactggcgc ttgcgcacat
    19021 ctaccagcgt cacacggtcg gtacaggagt agcagggatc aaggctaccg ataatcagcg
    19081 gtgcgtcaga aacggtattg ccgcgcagca tgtaacgcaa caccggccag ttggcgtagg
    19141 tggcggcacg gcagcgccag cggaacaatt tttggttatc accgagcatg ctccagtgca
    19201 cgtcttcacc gcgtggcgct tcaacaaagc ccagcgcgaa tgcgtgaggt ttatagctaa
    19261 agccttcggt cagcagtggg gtatccggca tgttgtcgag ggcaaattcc agcattgcca
    19321 gcgaatcaaa cgtctctttg acacggacca tcacgcggga gaaaacatcg ccgccggtaa
    19381 aggtaaacag tgtttttgga atattgccgt agtcggcgta ggggtgatca aagcgcaaat
    19441 cacgggcgat ttgtcggtcg agaatgccaa tgccctgagt gcgttgttcc atattcggcg
    19501 tagcaagcag catctctacc agctccgaca cgtcggcgcg catctcgcgc accagtttca
    19561 gcgtttgcag acgttgctct ttgagaatat cgcggcgaac accaccaatc agattcagac
    19621 cgtaggtttt acgcgacccg atcagcaatt ccgccatcgt catcgacttt tcccgcacgc
    19681 ggaaaaattg cataaagccg gtatcaaaac caacgaaatg gcaggagagg ccaaggttaa
    19741 gcaaatgact gtgtagccgt tcgacttcca gcagaatcga gcgaatagta tgtgctcgtt
    19801 gcggcacctc aatccccagt gcattttcaa ccgaattggt ataggccaca ctgtgggcaa
    19861 aaccgcaaat cccacacacg cggtccgata agaaggtcac ttcgttgtag cccatccgcg
    19921 tttctgccag tttctccatg ccgcgatgga cataaaacag gcggtaatca gcatcgacaa
    19981 tttgctcgcc atccacaaac aagcggaagt gacccggttc atcggaggtg atatgcagcg
    20041 ggccgacagg gatcacccgc gcatcgctgt tgccctcatt gataaacgca tacgtttcgg
    20101 aatctgtcgt cggttcagga cgcagtcgat aatccatcgc atctttgcgc agcggatgca
    20161 tatcttccgg ccagtcatcg ggcaacacca ggcgacgctg atccggcagg ccaaccggaa
    20221 tcagcccgta catatcgcga atttctcgct cgccccagac cgcggcaggg acgcgcggtg
    20281 tgactgacgg aaactcccga ctgtcggcat cgaccagtgc cttcaccacg atccagcatt
    20341 tttcggcccc ttccattgaa agggcataat aaaccgcgta atgaccgtta agtgtccgct
    20401 cgtcgttgcc aaacaggact ggaagccagc catcatgttg ataataaaga taatgtacaa
    20461 cgtcaggcag cagattgatt ttcaccgtaa tggtgacctg ttcaggcgtt tgtcgctctt
    20521 catccagcac cgcgccgggg aactgcgttt tcagggcggc gagaatcgct tcgccacgat
    20581 ttgacgatga attaacgttc actgtgtttc tccgaaacag atgcggtgcc tgaaggcaaa
    20641 aaatcatgcc aggtgctacg ttgtgcaggc aggtcgtgcg tccctgagag gactatagtg
    20701 gaagcgcccg ccaggatcct gatgacaggt tgtggaatat gcgttcccat cgccagcatc
    20761 atgaccagca gaatcaccat tggcgaggtg gtcaaccagc cgagatcacc ccggttaacg
    20821 gcctgcggcg gtttcgccat taacacccgc gcagccatcc gtaccaggcc cgccagcacc
    20881 agcgttaaca gcaataacag caggacgata atcagcaggt gattacgtgc cagtccggcg
    20941 gtaacggtca taaattcgct aagaaaaatg ttgaagggcg gcatccctgc cagcgccagc
    21001 gccagcgcac cgccgccaaa cagcacggcg gtaaatggca tgattttgag catcccacag
    21061 acgacgttga gatcgcgcgt gccgtacttg agcagtacat tgccggaacc gcagaacagc
    21121 agcgtttttg ccagactgtg gtttaaggtg tgcagcagcg cggcaaaaat tcccagtggc
    21181 ccgccaatgc ctagcgccac cgcgaccagt cccatgttct ccacgctgga gtacgccagc
    21241 agacgcttaa tgtcccgctg taccagaatg aaaaatgccg ccacggcaac cgacaacatg
    21301 ccgaagatga gcaacaaccg gttggggaaa tcgctgccga tggcctggca gataatgatg
    21361 taatagcgaa ttagcaccaa cagcgcgcag ttcagcaata cggcggagag cagggcgctg
    21421 accggactcg gtgcttcact gtgagcatcc ggcagccagg cgtgcatggg aaatagcccg
    21481 gttttggtgc caaagccaat tagcacaaac acaaaggcca acagcattaa tgttgggtca
    21541 agcaaggacg attgcttaag aacctcgctc cagaatatcg ccatttctgc ctgcggcata
    21601 acgctggcga cgttggcgta taccagcacg gtaccgaaca gaccaaaagc gacaccaaca
    21661 gtacaaataa tgatgtactt ccatgctgct tccagcgatg aacgctgacc gtaaatgcct
    21721 accagaaacg ccgagcttaa ggtggtggct tcgatcgccg cccacatcac aatcaggtta
    21781 ttgctggtaa caaccagcag catggtaaac aaaaacagat ggaagaaacc gtagtaatcg
    21841 cacagcgtaa cgggggaaag ctcgccgtgt gccacttcat gacgcatgta gccaatcgag
    21901 taaacaccgg tgagaaagcc aatcacacca agaatggcga ggaacaaacc gcccagacca
    21961 tcaatatgaa gccacagtcc cgcagcgaat atttctcctg catcagcggc agtttggacc
    22021 acccagagtg ccagaatcag cagcagtgtg atccctaagc tatgtaatac agtcaccgta
    22081 caagtcgcag agagcccccg tttccggcag gcaaaacaga gcagcgaaaa aagcagcggc
    22141 gtgagcagga gtaaagcgaa catcacagaa taactcatct cattatccct tcagcgcggt
    22201 caagttgttc acgtccagcg tgccgtgggt acgccatatt tttcttgcca gtaacaccat
    22261 cacaatgacg gcgaagatgg cgtcggtagc gatacctatt tccaccagtt ccggtgctcg
    22321 ccaggcaaga agcgccagca ccagatggga gccgttttcc atcaggcagt aaccaaaaat
    22381 ttgccgcagg atattgcgct ggctgacaat gcacagcagg ccaagcagaa aatgacctaa
    22441 cgctaccgcc agcgccggtt tcagcccggt agccatcggt agcttcacgg gctgaacgac
    22501 aaatgcgcaa agcaggacaa ttaacgccgc gagcagtgcc atcattgccg gaccgaataa
    22561 cgctttttcc gggatgttct ggggaatatt tcgtgcagcg taagtcatga ttaacggtac
    22621 cagcagcact ttggtgataa aggcactggc ggaccagatc agcagttgct ctgcggcgaa
    22681 caggcacgag agagtggcga aaatagacac cagcaccagc gactggcagg cgtaaaatcc
    22741 gcatgacagg cgatagcttt tgacgctaat cacaaacagc gatgtcagca tcatcagtcc
    22801 cgccagatta tttacgatca tagaaccggt cattcatcat ctccttactg caaccaggtc
    22861 gcggcgatta cgctggaaat cagcgacatc acaatcagta caatcaacac caggcgcatt
    22921 gaccctggca gcggtgcggc atcggcgacg gcctcgctcg gtttgccagg cacaacgcga
    22981 ccaaaccagc gaataaacca ggcgaaactg gcgaccgatt caatcatcag cagaatcatg
    23041 gcgggcagca ggatccagta ctccactgac aacgcaaaac aggcggcaaa cagcgggaat
    23101 ttactgaaga agccgttgaa cggcggcaca ccggtaatcg ccagcgctgc cacgcagaaa
    23161 cccacgcctg gcaacggcag ggtgtgcaat acgccacgca gacgcggcaa caagcgcgtg
    23221 ccgcagctgt aactcagcgc acctgctaca aggaaaaaca ggcttttagc gaacgcgtgg
    23281 ttgacgatgt aggcgatgct gccctccagt gccagccgcc cactttcacc atcgatgcgg
    23341 cgtggagata ggcgctgatc ggtgttggcg cttccattgc gtccggtagc cacgcttgca
    23401 tcggtagctg ggccgatttc ccccacgcgg caaacaggat gccgccataa accagataac
    23461 gtgcgtcgcc atgtaactcg ctcatcgcgc taagcacaaa cgttccggtc tgcaaaaaca
    23521 gcgtggcggc ggcaagatac aaccccaacg aaccgatatg ggtgataagc aacgctttta
    23581 gtgctgaacg ctgcgcttta tcgctctggt aataactgat caacgcccag gagcagccgc
    23641 cctaatttca aaaaacaaca actgaccgag cagcgtcgag gagagtacca gtcccgccat
    23701 cgcgccgata aacaccagca aaaatgcgta ataacgattc gtgccgttat gcgggtgttc
    23761 gcgattttta tccgtcagat aacccgtgga gtagatcgtg accagcaaac cgagaaacac
    23821 caccacaaac agaatcagcg tactcacgcg atcaatgacc aggccaaaca atgccacatc
    23881 ccccacgttg accaacgtca acgtgacggc aactttatcg gcctgataaa acgcggagat
    23941 cagcgacaac atgcacagcg tggtcagtgc ggcgaataaa acccccattc ggcggcccga
    24001 cgttgtggcg aaaacgaaac gaccagtgcg ccgataaaag gcagcattaa cgtcgtcaga
    24061 gcaagatttt ccatattccg tcagtgctcc ttacagaccg gttaaccaga agacccatgc
    24121 aagcgcagca aggctgaagc caagccaggt cacatggtga atgagtaaaa aacgcccgcg
    24181 tgccagcgtg ttttctgcga ttgaggccag tacaaaaatc agcaaaacct tgagcagcgt
    24241 aacgacaagt gaagtcagca ggcaggcgag agaaagttct tgcgcgcgcc caaagagcag
    24301 aaacagggcc acaaacagtg ctgccatcac gacctgtttc agccccagcc cccatttcgc
    24361 tagcgccagc ccggcaccgg aatattcggt cagcgggcct tcctgtaatt cctgttctgc
    24421 ttcagcaaca tcaaagggaa tttttcccat ctcaatgaag caggcaaaac cacaggccag
    24481 taacgccagt acggtggtta gcggcgagtt ccagcccgtt gccagcgtat tgctgatcat
    24541 ctcgatatgc gtggaacctg ctatcagcgc caataccagc agtgagagaa tgagcatggg
    24601 ttcgaccaga atgccgagcg tcaactcgcg actggcaccg actcccgcaa acgggcttcc
    24661 ggtatccagc ccggaaagag caaagaaaaa acgaaacagg gcaagaagat agataagggt
    24721 gatcagatcg ccgccgcccg caaaagggga aacggtaata aaaagtggta gggccatcgc
    24781 cagcaccagc atgctgctga ttaataccca cggcatcagg cggaacatca gacctgaaga
    24841 tataggcgca acttcctggc gtttaaacag tttgtggata tcgcgataat cctgccagat
    24901 ccccggcccg cggcgggagt gcatacgcgc gcgtatctgc cgggaaatac ccgtaaaaag
    24961 cggggttagc agcagaatca cggcctgtgc taacgcaaaa atgaccagat atccgtcgca
    25021 aagagtttgt ctcatggtga tttccttaga cggcaatagc gattagcagc acaaccagtg
    25081 cggcgaccac atacaggcaa tagagacgaa agtcgccgct ttgcagatgc tgtatttctt
    25141 tggccagccg ttgggtggcg ctcacgatgg ggcggatcac ccgctcatcc cagaagggtt
    25201 ctgtgctctg agccctggcg gtgacgtgcg caagaccttt atttagcctc agcgtagggt
    25261 cgaactgttg tcgtagacga aatagcgcag aaaagaccac acgcagcggc tgcatcacgc
    25321 cattgcctga cggggccatc gcattttccc agccgtagcc gcatgcccag gcatctcctg
    25381 tgcgacgaaa cgctgcgcgg cgcgaacgac accacagcca gtaaaggcca ggcagcaaag
    25441 gcatcgccag tagtaacagc aacagtaatg agggggtgac ccgtgtatga aacgtgccgg
    25501 gtacaagtgc tattccgctg gcgacagtga cgggaggggt attggtaaac gcatgggcaa
    25561 tatgcattat cttcggtgcc agccaactgg cactaagcgc aattaatacg cagagtgccg
    25621 cgagcagtag catggcgacg atcatcgtat ttggcacttc ctgtgcctct tcagcgtgtg
    25681 tactgcgcgg cgcaccacag aaagtaatac cgtacatttt gacgaagcac attactgcca
    25741 gcccaccagt gactgccagc attaccatag caataggacc cgcaagttgt agcgctacgg
    25801 cttccacacg acttagtgag aacagcgact gccaggtgta ccattcgctg ataaaaccat
    25861 tcagcggagg aatggctgat atcgcgaggc aaccaatcag gcatgctgcg gctgtccacg
    25921 gcatccgttt cgccagtgcc cccatttttt ccatgtcgtg ggtatgcaaa cgcgaaataa
    25981 tcgcacccgc gccgagaaat agcagccctt tgaacagcgc atggttgagc agatgaaaca
    26041 gtgcgccgag cagtccaacc acggtgagca gcgggtcgtg cagtgacaga ccgtccatcg
    26101 ccacaccgac cgccagcaaa ataatgccga cgttttcgac ggtactccag gccagcagcc
    26161 gtttgatatc ctgttccgcc agtgcataca gcacgcccag gagcgcggag attgcgccga
    26221 tcgccatcac cagagtgccc cactacagag gcaaacccgt ttgcgccagc agatccatcg
    26281 ctactttcag gatgccgaaa ataccgattt tgaccattac gccagacatc aacgccgaag
    26341 cgtgcgatgg tgcggcaggg tgagcgcgcg gcaaccagct gtgcaacggc atcatcccgg
    26401 ctttcgcgcc aaaaccgaaa aaggccagca gaaacaccgc cgacgccagc cccggagaaa
    26461 gtgaaagcgt gcgaaactgg caaaatcgag gctgccgctt tcgcgcccca tcagcaagaa
    26521 ggcgatcata atcagcaccg atccggcgtg ggcgataaaa aagtagagca tgccagcgtt
    26581 gatcgacgtt ttatcctgcc tggcaatgac cagaaaccag gaagacagcg acatcatttc
    26641 aaacagcacg atgaaccaaa aagcgttgtc catcaccagc agggcaacca tcgatgcgat
    26701 gaaaagattc ataaagaagc ccatcgccgc cgcgcctttg ccctcgtatt cgcgcatata
    26761 agtcaatgaa tagagcgaac aaaccaccac cagcaaggag ataaccagca ccataaatgc
    26821 cgagaggcta tccattcgca gggttaaatc ggcaaagtca aaaggggtgg caaaatgggc
    26881 gactaaagtt acaccagcat gtaattgcgt aattccgctg atgatgccaa tcacgccgcc
    26941 caccagcgat gtgatgccgg aaagcttaat agccagtctg tccagaccga gtaaaaacag
    27001 cgaagccaga ctggcaaaca gatagaggat cagcgaccag gttaataatt gcagggcatc
    27061 cattagtgtt gctcctgagt gagggaaagg ggatcctccc cgccattggc aaaccagctt
    27121 gcggcaaggc gctgtttttc tttcatctga cgttgcaggc tatcgtcggt gatcagccgt
    27181 aacgcctgat tcgggcaagc gcgaacgcag gccggacctt ctggcaagaa atcacacagg
    27241 tcgcatttca ccgcgacggt ctggacaccc ggttcccagc gcagcaacgc atgttggggc
    27301 agcacgtttt cttcgccgtc tttgagtgag ccttcagcct gaaaaacata ttgcgcatgg
    27361 gcattcaccg gacggcttcc tgaagcgctg attgcgccaa atgggcagac cacggcgcaa
    27421 agcttgcagc caatacagag gctttcgttg agttggatcg catcatccct ctgagagatg
    27481 gcattgaccg ggcagacctg caggcaaggg gcttcctcac agtgatgaca cacgacaggg
    27541 gcagtgattg ttgacgtctt cgccagggcc aggcgcgggt gttgctgtaa accttgcgtt
    27601 ttatgcacgt ccgaacaggc agcgagacag gtattacatc ctgtacacca cagtggttcg
    27661 gccaccacaa agcggttcat gtcggctcct tgaaaatgat atgaaacatt gcatttcata
    27721 ttgcataaag gatgccattc tgattaactc aatatattca ataagttatg tattgactgg
    27781 attctgaacg gtacaaaaat gatggtcatt aacgttcagc aaaaaatgga ttcgacaccc
    27841 gtgtcgcagg ggaaagcgag aatgtgatct tgatcacgag aagtgaggta tgctggccgc
    27901 aagcgcagcc agcacggaat ggagcaaagt aatcaggcgt gttctttcag ccagttcagc
    27961 acaacgtcgt ggtgattgct ggttttgaaa tcgtcaaaga catgttcgat tttgccatca
    28021 gcgtcaatca ggaagctgat gcgatgaatg ccatcgtagg ttttgcccat gaaggacttt
    28081 tcaccccaga cgccgaattg ttcgcacacc tggtggtcct catcagacag gagcgtaaag
    28141 ttaagcagct ctttttccgc aaaacgggag agtttttcgg gtttatcggt gctgataccc
    28201 agcacatcaa cgcccgcttt tttcaactca tccatgttat cgcgtaagcc gcaggcctgt
    28261 acggtacagc cgggggtcat ggctttcggg tagaaataaa ccagaacacg ctgtccctgg
    28321 aagtcggtca aattaacttg ttctccgtct tgatccggca agctaaattt cggtgcgata
    28381 tcaccggctt tcagtggatt cattacttaa ctccatcctg ttcatcatgt tgggaataat
    28441 tgacgacgtt aatactgcct tgtgcattga gttctgtaca tagggctttg aacgcttgct
    28501 caatatttgc tgcgtccgca gatgcggggc tgtgggcggt tatctgaata tgcaactgcg
    28561 cagcccgttc attttcagca ggttgcgtgc gcgacaccag ctccgcaatg ttcatatgat
    28621 gcgcgtcgaa aagtgctgtg aagcgttcaa ttaaatgcgg ggagtctgcc acatcgacct
    28681 gaacccagac agatgctggc attggcggac gcggacgcgc cgtcgtgcgc ttcatcacga
    28741 ttaaaagatc cagttcggca cctttcaacg gtaacgttga ttcaatcaga gtaatggcat
    28801 tccatgaacc ggaaagcagc ataataaacg tgaactcttc tcccagcatc gccaggcgac
    28861 tgtcttcaat attacagccg caactactga catgacgggt gatggtgttc acaattccag
    28921 ggcgatcggc acccaacgca gtgatcacca gataatgttg cgatgacagt gtcaaactgg
    28981 ttattccttt aaggggtgag ttgttcttaa ggaaagcata aaaaaaacat gcatacaaca
    29041 atcagaacgg ttctgtctgc ttgcttttaa tgccatacca aacgtaccat tgagacactt
    29101 gtttgcacag aggatggccc atgttcacgg gaagtattgt cgcgattgtt actccgatgg
    29161 atgaaaaagg taatgtctgt cgggctagct tgaaaaaact gattgattat catgtcgcca
    29221 gcggtacttc ggcgatcgtt tctgttggca ccactggcga gtccgctacc ttaaatcatg
    29281 acgaacatgc tgatgtggtg atgatgacgc tggagctggc tgatgggcgc attccggtaa
    29341 ttgccgggac cggcgctaac gctactgcgg aagccattag cctgacgcag cgcttcaatg
    29401 acagtggtat cgtcggctgc ctgacggtaa ccccttacta caatcgtccg tcgcaagaag
    29461 gtttgtatca gcatttcaaa gccatcgctg agcatactga cctgccgcaa attctgtata
    29521 atgtgccgtc ccgtactggc tgcgatctgc tcccggaaac ggtgggccgt ctggcgaaag
    29581 taaaaaatat tatcggaatc aaagaggcaa cagggaactt aacgcgtgta aaccagatca
    29641 aagagctggt ttcagatgat tttgttctgc tgagcggcga tgatgcgagc gcgctggact
    29701 tcatgcaatt gggcggtcat ggggttattt ccgttacggc taacgtcgca gcgcgtgata
    29761 tggcccagat gtgcaaactg gcagcagaag ggcattttgc cgaggcacgc gttattaatc
    29821 agcgtctgat gccattacac aacaaactat ttgtcgaacc caatccaatc ccggtgaaat
    29881 gggcatgtaa ggaactgggt cttgtggcga ccgatacgct gcgcctgcca atgacaccaa
    29941 tcaccgacag tggtcgtgag acggtcagag cggcgcttaa gcatgccggt ttgctgtaaa
    30001 gtttagggag atttgatggc ttactctgtt caaaagtcgc gcctggcaaa ggttgcgggt
    30061 gtttcgcttg ttttattact cgctgcctgt agttctgact cacgctataa gcgtcaggtc
    30121 agtggtgatg aagcctacct ggaagcggca ccgcttgcgg agcttcatgc cccggctgga
    30181 atgattttgc cggtgacctc cggtgattat gcaatcccgg tgaccaacgg tagtggtgct
    30241 gtcggtaagg cgctggacat tcgtccacca gcccagccgc tggcactggt ttctggcgcg
    30301 cgtacccagt tcacgggcga taccgcttca ttgctggtgg aaaatggtcg tggcaatact
    30361 ctgtggccgc aggtggttag cgtgctgcag gcgaaaaact acaccatcac ccaacgtgat
    30421 gatgctggtc agacactgac caccgattgg gtacaatgga accgtctgga cgaagacgag
    30481 cagtatcgtg gtcgttatca aatctctgtt aagccgcagg gttatcagca ggcggttacg
    30541 gttaaactgc tgaacctgga acaggcgggc aaaccggttg cagacgcggc ttccatgcag
    30601 cgttacagca cggagatgat gaacgttatt tccgccggtc tggataaatc tgccactgac
    30661 gccgcgaacg ctgcgcaaaa tcgtgcctcc accactatgg acgtacaaag tgcagctgat
    30721 gacaccggtt taccgatgct ggtcgtacgc gggccgttca atgtggtttg gcaacgtctg
    30781 ccagcggcgc tggaaaaagt gggcatgaaa gtgaccgaca gcacccgttc gcagggtaac
    30841 atggccgtaa cttataagcc gctgtctgac agcgactggc aggaactggg cgcgagcgat
    30901 ccaggcctgg catccggtga ctataaactg caggttggcg atttagataa ccgcagcagc
    30961 ctacagttca tcgatccgaa aggtcatact ctgactcaga gtcagaacga cgcgctggta
    31021 gctgtcttcc aggctgcgtt tagcaagtaa aaatacaggg ctggaatcat ccggcccttt
    31081 tttctgatat gatacgcaaa cgtgtgcgtc ggcaggaaaa cgcgatttta gcggtaattc
    31141 gcacgaaatt tgtttgtcgg acgtagttcg gataaggcgt tcacgccgca tccgacaaaa
    31201 catccggcac accagacagc aaaagatttt aaaacgttaa ttcacaccca ggagtgataa
    31261 agatgcaaaa gcaagctgag ttgtatcgtg gtaaagcgaa aaccgtatac agcacggaaa
    31321 acccggacct gttggtgctc gaattccgca atgatacgtc agcaggggat ggcgcgcgca
    31381 ttgagcagtt tgatcgcaaa ggtatggtga acaacaagtt caattacttc attatgagca
    31441 aactggctga agcgggtatc ccgactcaaa tggagcgtct gctctccgat accgaatgtc
    31501 tggtgaaaaa gctggatatg gtgccggttg agtgtgtcgt gcgtaaccgt gctgctggct
    31561 ctctggtgaa acgtcttgga atcgaagaag gtattgagct gaacccgccg ctgttcgatc
    31621 tgttcctgaa aaacgacgcc atgcacgatc cgatggtcaa cgaatcttac tgcgaaacct
    31681 ttggctgggt gagcaaagag aacctggcgc gtatgaaaga gctgacctac aaagcgaacg
    31741 acgtgctgaa aaaactgttc gatgatgctg gtctgattct ggtcgacttc aagctggagt
    31801 ttggcctgta caaaggtgaa gtggtactgg gcgatgagtt ctccccggac ggcagccgcc
    31861 tgtgggacaa agaaacactg gagaaaatgg acaaagaccg tttccgccag agcctcggtg
    31921 gcctgatcga agcctatgaa gccgtcgccc gccgcctggg tgtacagctg gactgatttt
    31981 tctggttgct tgtttcttta gccggagggt actccggcta aatgtctttg atacccccgc
    32041 tacaatactt ctcattatct ccctttgaca ggaaagtcat accaggccaa tatgtatata
    32101 cataacgtat atcccaaaaa gggggggcta tggaaactac tgtattttta agtaatcgca
    32161 gtcaggccgt cagattaccg aaagcggtcg ctctgccaga agacgttaag aaagtagaaa
    32221 ttattgcgat tggtcgtaca agaatcatca ctcccgccgg tgagtcctgg gacagttggt
    32281 ttgatggcga aaatgtgagt gcggatttta tggatatccg tgatcaaccc gcaatgcagg
    32341 aaagggaatc attttgatgc tgaaattcat gctggatacc aatatctgta ttttcactat
    32401 taagaataaa ccagtacatg tcagagagcg gtttaacctg aattctggcc ggatgtgtat
    32461 aagttctgta acattgatgg agcttatcta tggggcagaa aaaagtcaga tgccagaacg
    32521 caatttggcg gttattgaag gtttcgtatc tcgtttggta gtgcttgatt atgatagtgc
    32581 tgctgcgacg cataccgggc aaatcagggc cgagcttgcc cggcagggaa ggcctgttgg
    32641 accttttgac cagatgatag cgggacatgc tcgcagtcgc ggtttgattg tggttaccaa
    32701 caatacccga gagtttgagc gagtggcggg aatcagacta gaagactgga gctaacattc
    32761 tcttttactt ctaacattcg taaattatat aacgtggcat gaagccttaa ttcctccgcc
    32821 atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca tttctataat
    32881 gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga caatgttgaa
    32941 gacaggcgca acagctccgg tggtccgtct atgggcggtc ctggttttcg cctgccaagc
    33001 ggtaaaggcg ggctgatttt actgttagtc gtgctggttg caggctacta tggtgttgat
    33061 ttaaccgggt taatgactgg acagccggtt tcccaacaac aatcaacgcg gtccattagc
    33121 cctaatgaag acgaagctgc aaaattcacc tcggtgattc tggcaaccac ggaagacacc
    33181 tggggacaac agttcgagaa gatgggtaag acctatcagc aaccgaagct ggtcatgtac
    33241 cgtggaatga cgcgcaccgg ctgcggggct ggtcagtcca ttatggggcc gttctattgc
    33301 ccggcggatg gcacagttta tatcgatctc tccttctatg atgacatgaa agacaaactt
    33361 ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg tcatcatgtg
    33421 cagaaactgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc gacgcaggcg
    33481 gaagtgaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc cggtgtctgg
    33541 gggcataaca tgcagcagca aggcgttctg gaaaccggcg atctggaaga ggcgctgaac
    33601 gcggcgcagg ccatcggcga tgaccgttta caacagcaaa gtcaggggcg agtagtacca
    33661 gatagtttca cacatggcac ttctcagcaa cgctacagct ggtttaaacg tggtttcgac
    33721 agcggcgatc cggcacaatg caatactttt ggtaaaagca tttaacttcc ggggcaggga
    33781 tggctgaact gactgcgctt cacacattaa cagcgcaaat gaaacgtgaa gggatccgcc
    33841 gcttgctggt gttgagcggg gaagagggtt ggtgttttga tcatgcgctt aagttgcgtg
    33901 atgccttacc tggcgactgg ctgtggattt cgccgcagcc agatgctgaa aaccactgtt
    33961 caccctcggc gctacaaact ttacttgggc gcgagttccg gcatgcggta ttcgacgccc
    34021 gccacggctt tgatgccgct gcctttgcag cacttagcgg aacgttgaaa gcgggaagct
    34081 ggctggtttt gttactccct gtatgggaag agtgggaaaa ccaacctgat gccgactcgc
    34141 tgcgctggag tgattgccct gaccctattg cgacgccgca ttttgtccag catctcaaac
    34201 gcgtacttac ggcggataac gacgctatcc tctggcggca aaaccagccg ttctcgttgg
    34261 cgcattttac tccccgtact gactggcacc ccgcgaccgg cgcaccacaa ccagaacaac
    34321 agcaactctt acagcagtta ctgaccatgc cgccgggcgt ggcagcggta acggctgcgc
    34381 gtgggcgcgg taaatcggcg ttggcagggc aactcatttc tcgtattgcg ggcagtgcga
    34441 ttgtcaccgc gcccgcaaaa gcggcaacgg atgtattggc acaatttgcg ggcgagaagt
    34501 ttcgctttat tgcaccggat gccttgttag ccagcgatga gcaagccgac tggctggtgg
    34561 tcgacgaagc cgcagccata cctgcgccgt tgttgcatca actggtatcg cgttttcctc
    34621 gaacgttgtt aaccactacg gtgcagggct acgaaggcac cggacgtggt tttttgctga
    34681 aattttgcgc tcgctttccg catttacacc gttttgaact gcaacagccg atccgctggg
    34741 cacagggatg cccgctggaa aaaatggtta gtgaggcact ggtttttgac gatgaaaact
    34801 tcacccatac accacaaggc aatatcgtca tttccgcatt tgaacaaacg ttatggcgaa
    34861 gcgagccaga aacgccgtta aaggtttatc agttattgtc tggtgcgcac taccggactt
    34921 cgccgctgga tttacgccgg atgatggatg caccagggca atatttttta caggcggctg
    34981 gcgaaaacga gattgccgga gcgctgtggc tggtggatga ggggggatta tctcaacaac
    35041 tcagtcaggc ggtatgggca ggttttcgtc gcccgcgggg taatctggtg gcccagtcgc
    35101 tggcggcgca cggtagcaat ccactggcag cgacattgcg tggacggcgg gtcagccgga
    35161 tagcagttca tccggcgcgt cagcgggaag gcacagggca gcaacttatt gctggtgctt
    35221 tgcaatatac gcgtgacctc gactatcttt cggtgagttt tggttacacc ggggagttat
    35281 ggcgtttctg gcaccgctgc ggttttgtgc tggtgcggat gggtaatcat cgtgaagcca
    35341 gcagcggttg ctatacggcg atggcgctgt taccgatgag taatgcgggt aaacaactgg
    35401 ctgaacgtga gcattaccgt ttacgtcgcg atgcgcaagc tctcgcaaag tggaatggcg
    35461 aaacgcttcc tgttgatcca ctaaacgatg ccgtcctttc tgacgacgac tggcttgaac
    35521 tggccggttt tgctttcgct catcgtccgc tattaacatc gttaggttgc ttattgcgtc
    35581 tgctacaaac cagtgaactg gcattaccgg cgctgcgtgg gcgtttacag aaaaacgtca
    35641 gcgacgcgca gttatgtacc acacttaaac tttcaggccg caagatgtta ctggtccgtc
    35701 agcgggaaga ggccgcgcag gcgctgttcg cacttaatga tgttcgcact gagcgtctgc
    35761 gcgatcgcat aacgcaatgg caattttttc actgactcct tcaattttct cccatggtca
    35821 ttgtgagtca gcggcgtaaa attaatgaca tcagttaagg agttcgccat gaaacatgac
    35881 cattttgttg ttcaaagccc ggataaacct gcgcaacagt tgttactgct tttccacggt
    35941 gtcggggata acccggtggc aatgggggaa ataggtagct ggtttgcacc gctgtttcct
    36001 gatgcgctgg tggtgagtgt cggtggcgcg gagccgagcg gtaatccggc ggggcgtcag
    36061 tggttttcgg tgcagggtat tacggaagat aatcgccagg cgcgtgtgga tgcgatcatg
    36121 ccgacgttta ttgaaacggt gcgctactgg cagaaacaga gcggagtagg ggctaacgcc
    36181 acggcgctca tcggtttctc tcaaggcgcg attatggcgc tggagagcat taaagccgaa
    36241 ccgggccttg cttcacgcgt cattgctttt aacggacggt atgcgagcct gccggagact
    36301 gcgtctacag ccacgacaat tcacctgatt catggcggtg aagatccggt tatcgatctg
    36361 gcgcatgcgg ttgcggcgca ggaagcgtta atcagtgccg gtggtgacgt gacgctggat
    36421 atcgtggagg atttaggcca tgcgatcgac aatcgcagta tgcaatttgc cctggatcac
    36481 ttgcgttata ccattccgaa acattatttc gacgaagcgc taagtggcgg taagcctggc
    36541 gatgatgatg tgattgaaat gatgtaaaag acagattacc tggcctgata agacgcgtga
    36601 gcgtcgcatc aggcatcgtt caccattgcc ggatgcggcg ttaacgcctt atccggccta
    36661 cggttgagca ctcagcttac ttcttcggtt gatccttttt cggccagtcg tcgtcatcgt
    36721 cccacttatc gttgaaatca cgatgcgggg gaagctccgg cttgttggcg agaaatttct
    36781 tatggtcgac gcgcttgaga tctttaatca cgttcagcag gacgcctacc aaaaagacaa
    36841 tcaccagaat ccaccaatat ttagccagcc agtccatgct tatttcctct tgcagaacca
    36901 ctcatcaggc gacgagctgt tccatgatac gttgatacat acgggcaagt agctgcaggt
    36961 cggcagcgtt cacacattca ttaattttat gaatagtggc attgaccggc ccgagttcca
    37021 ccacctgcgc ccccatgcga gcaataaagc gcccgtcgga cgttccgcct gtggtcagta
    37081 gctgcggttt aatttcatta tagtgctcaa ccgcgttaac gaccgcatcc accagtttac
    37141 cgcgcgcggt caaaaatggc tgcccggaaa gccaccagtc caccgtataa cgcagttgat
    37201 gtttttcaag cagggcaagc acctgtgctt tgatcatctc atcagtcagt tcggtgctga
    37261 agcggaagtt aaactgcaca aacagttcac ccggaataac attgttactg cccgttcccg
    37321 cctgaatatt agcaatctgc atactggtcg ccgggaaaaa ttcattgccc tgatcccact
    37381 caatagccac taattcatta aggaaaggtg ctgcgcgatg tactggattg tcagccagat
    37441 gcgggtaggc cacatgcccc tgaacgccat gaatggtaag gttgcaggtt aacgatccgc
    37501 gacgaccatt tttcaccaca tcaccaacca cttcgatact cgacggttcg ccgaccaggc
    37561 agtaatcgag acgctcatta cgtgccatca acgcttcgac gacttttacc gtaccgttgt
    37621 gggcactggc ttcttcatca gaggtgatca gaaatgccag tcgccccgta tggttgggat
    37681 gttgtgcgac aaagcgttct gccgctacca ccatcgccgc cagcgagcct ttcatatctg
    37741 ccgcaccgcg tccgaataac atgccatcac gaatggtggg ttcaaacggc ggattgatcc
    37801 aacgatcggc gtcgccaggc ggcaccacgt cggtatgccc ggcaaaggcc aacgtttcac
    37861 cttgcccacg ccatgcccaa aaattctgcg tatcggcaaa atccatgcgt tcaacggtaa
    37921 aaccgatcgc ctgcaaacgt tcaatcaaca aagcctggca tcctgcgtca tcaggactca
    37981 gggaagggcg gcgaataagc tgttgtgtca gctcaataac cgggcacgac atagactaca
    38041 cctcatggaa aaattgctga taactggaat cactgaaacc cagcagcata ggcttaccag
    38101 gcgcgcagag caatggacgt ttgataattg caggcatttc agtcattaat gccgccgcag
    38161 aggccgcatc ggtgatttta ttgcgggtgg tttcgtccag tttacgccag gttgtaccac
    38221 gggtgttcag taacgcttcc cagcctaatt cgttgataaa accgttcaat aattcgctgt
    38281 ccagcccatc gacgcggtaa tcatgaaaac gatagtcgat gttattggct tccagccaac
    38341 ggcgagcctt tttaatggtg tcacaatttt tgatgccgta aagtgtaacc ataatgaatc
    38401 ctttttcgat ctatgagaat aaatactcat ttaagggtga aaacattttg cctgcaaat
//
LOCUS       DBANUU010000032        37716 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000032 DBANUU010000000
VERSION     DBANUU010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.032"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(32..1891)
                     /gene="gss"
                     /locus_tag="ACIE68_001580"
     CDS             complement(32..1891)
                     /gene="gss"
                     /locus_tag="ACIE68_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFX5907913.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2096..2962
                     /gene="yghU"
                     /locus_tag="ACIE68_001581"
     CDS             2096..2962
                     /gene="yghU"
                     /locus_tag="ACIE68_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFX5907914.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(2991..3072)
                     /locus_tag="ACIE68_001582"
                     /pseudo
     CDS             complement(2991..3072)
                     /locus_tag="ACIE68_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3085..3333)
                     /gene="hybG"
                     /locus_tag="ACIE68_001583"
     CDS             complement(3085..3333)
                     /gene="hybG"
                     /locus_tag="ACIE68_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFX5907915.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3346..3687)
                     /gene="hypA"
                     /locus_tag="ACIE68_001584"
     CDS             complement(3346..3687)
                     /gene="hypA"
                     /locus_tag="ACIE68_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFX5907916.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCCQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3680..4168)
                     /gene="hybE"
                     /locus_tag="ACIE68_001585"
     CDS             complement(3680..4168)
                     /gene="hybE"
                     /locus_tag="ACIE68_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFX5907917.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4161..4655)
                     /gene="hybD"
                     /locus_tag="ACIE68_001586"
     CDS             complement(4161..4655)
                     /gene="hybD"
                     /locus_tag="ACIE68_001586"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFX5907918.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4655..6358)
                     /gene="hybC"
                     /locus_tag="ACIE68_001587"
     CDS             complement(4655..6358)
                     /gene="hybC"
                     /locus_tag="ACIE68_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFX5907919.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6355..7533)
                     /gene="hybB"
                     /locus_tag="ACIE68_001588"
     CDS             complement(6355..7533)
                     /gene="hybB"
                     /locus_tag="ACIE68_001588"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFX5907920.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7523..8509)
                     /gene="hybA"
                     /locus_tag="ACIE68_001589"
     CDS             complement(7523..8509)
                     /gene="hybA"
                     /locus_tag="ACIE68_001589"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFX5907921.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8512..9630)
                     /gene="hybO"
                     /locus_tag="ACIE68_001590"
     CDS             complement(8512..9630)
                     /gene="hybO"
                     /locus_tag="ACIE68_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFX5907922.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9819..10106)
                     /gene="yghW"
                     /locus_tag="ACIE68_001591"
     CDS             complement(9819..10106)
                     /gene="yghW"
                     /locus_tag="ACIE68_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFX5907923.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10225..11112)
                     /gene="yghX"
                     /locus_tag="ACIE68_001592"
     CDS             complement(10225..11112)
                     /gene="yghX"
                     /locus_tag="ACIE68_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFX5907924.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11269..12309
                     /gene="gpr"
                     /locus_tag="ACIE68_001593"
     CDS             11269..12309
                     /gene="gpr"
                     /locus_tag="ACIE68_001593"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFX5907925.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12349..12843)
                     /gene="yqhA"
                     /locus_tag="ACIE68_001594"
     CDS             complement(12349..12843)
                     /gene="yqhA"
                     /locus_tag="ACIE68_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFX5907926.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13034..13918
                     /gene="yghA"
                     /locus_tag="ACIE68_001595"
     CDS             13034..13918
                     /gene="yghA"
                     /locus_tag="ACIE68_001595"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFX5907927.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14190..14615)
                     /gene="exbD"
                     /locus_tag="ACIE68_001596"
     CDS             complement(14190..14615)
                     /gene="exbD"
                     /locus_tag="ACIE68_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFX5907928.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14622..15356)
                     /gene="exbB"
                     /locus_tag="ACIE68_001597"
     CDS             complement(14622..15356)
                     /gene="exbB"
                     /locus_tag="ACIE68_001597"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFX5907929.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15608..16795
                     /gene="metC"
                     /locus_tag="ACIE68_001598"
     CDS             15608..16795
                     /gene="metC"
                     /locus_tag="ACIE68_001598"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFX5907930.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16935..17594
                     /gene="yghB"
                     /locus_tag="ACIE68_001599"
     CDS             16935..17594
                     /gene="yghB"
                     /locus_tag="ACIE68_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFX5907931.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17634..18533)
                     /gene="yqhC"
                     /locus_tag="ACIE68_001600"
     CDS             complement(17634..18533)
                     /gene="yqhC"
                     /locus_tag="ACIE68_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFX5907932.1"
                     /translation="MKREEICRLLADKVNKLKIKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18727..19890
                     /gene="yqhD"
                     /locus_tag="ACIE68_001601"
     CDS             18727..19890
                     /gene="yqhD"
                     /locus_tag="ACIE68_001601"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFX5907933.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWGATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19995..20822
                     /gene="dkgA"
                     /locus_tag="ACIE68_001602"
     CDS             19995..20822
                     /gene="dkgA"
                     /locus_tag="ACIE68_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFX5907934.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21022..21948
                     /gene="yqhG"
                     /locus_tag="ACIE68_001603"
     CDS             21022..21948
                     /gene="yqhG"
                     /locus_tag="ACIE68_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFX5907935.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21999..22256
                     /gene="yqhH"
                     /locus_tag="ACIE68_001604"
     CDS             21999..22256
                     /gene="yqhH"
                     /locus_tag="ACIE68_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFX5907936.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22299..24518)
                     /gene="ygiQ"
                     /locus_tag="ACIE68_001605"
     CDS             complement(22299..24518)
                     /gene="ygiQ"
                     /locus_tag="ACIE68_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFX5907937.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24629..26041)
                     /gene="ftsP"
                     /locus_tag="ACIE68_001606"
     CDS             complement(24629..26041)
                     /gene="ftsP"
                     /locus_tag="ACIE68_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFX5907938.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26116..26853)
                     /gene="plsC"
                     /locus_tag="ACIE68_001607"
                     /gene_synonym="parF"
     CDS             complement(26116..26853)
                     /gene="plsC"
                     /locus_tag="ACIE68_001607"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFX5907939.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27087..29345)
                     /gene="parC"
                     /locus_tag="ACIE68_001608"
     CDS             complement(27087..29345)
                     /gene="parC"
                     /locus_tag="ACIE68_001608"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFX5907940.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29483..31090)
                     /gene="ygiS"
                     /locus_tag="ACIE68_001609"
     CDS             complement(29483..31090)
                     /gene="ygiS"
                     /locus_tag="ACIE68_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFX5907941.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQTPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31223..31618)
                     /gene="mqsA"
                     /locus_tag="ACIE68_001610"
     CDS             complement(31223..31618)
                     /gene="mqsA"
                     /locus_tag="ACIE68_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MqsA"
                     /protein_id="HFX5907942.1"
                     /translation="MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESI
                     MNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKKLSLTQKEASEIFGGGVNAFSRY
                     EKGNAQPHPSTIKLLRVLDKHPELLNEIR"
     gene            complement(31620..31916)
                     /gene="mqsR"
                     /locus_tag="ACIE68_001611"
     CDS             complement(31620..31916)
                     /gene="mqsR"
                     /locus_tag="ACIE68_001611"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin MqsR"
                     /protein_id="HFX5907943.1"
                     /translation="MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCN
                     VIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQVYLKITVIHDVLIVSFKEK"
     gene            complement(32121..32603)
                     /gene="ygiV"
                     /locus_tag="ACIE68_001612"
     CDS             complement(32121..32603)
                     /gene="ygiV"
                     /locus_tag="ACIE68_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFX5907944.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(32656..33048)
                     /gene="ygiW"
                     /locus_tag="ACIE68_001613"
     CDS             complement(32656..33048)
                     /gene="ygiW"
                     /locus_tag="ACIE68_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFX5907945.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            33200..33859
                     /gene="qseB"
                     /locus_tag="ACIE68_001614"
     CDS             33200..33859
                     /gene="qseB"
                     /locus_tag="ACIE68_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFX5907946.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPDKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            33856..35205
                     /gene="qseC"
                     /locus_tag="ACIE68_001615"
     CDS             33856..35205
                     /gene="qseC"
                     /locus_tag="ACIE68_001615"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFX5907947.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNRLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            35315..35896
                     /gene="mdaB"
                     /locus_tag="ACIE68_001616"
     CDS             35315..35896
                     /gene="mdaB"
                     /locus_tag="ACIE68_001616"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFX5907948.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            35927..36241
                     /gene="ygiN"
                     /locus_tag="ACIE68_001617"
     CDS             35927..36241
                     /gene="ygiN"
                     /locus_tag="ACIE68_001617"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFX5907949.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(36285..37616)
                     /locus_tag="ACIE68_001618"
     CDS             complement(36285..37616)
                     /locus_tag="ACIE68_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5907950.1"
                     /translation="MKLTINEKIGFGAGDMAIAIVMMSLAMIITYFYTDVFGLKPVDL
                     GILLFSVRILDAVIDPVVGTMTDRTNTRWGKYRPWLLFMSIPFGISIWLMFTTPDTDY
                     SVKLLWAWATYVLLTLTYTLIAIPYVSLISVITDDPQERLSANGYRFVMTKIAMFAVT
                     IIVPLSAMYLGKNNVKLGYQIAMGAMGILATCLCLYCFFSVRERIYHPKPSLGMAAQF
                     RLLIKNDQWLILGAVIAIIMFGGIIRNSVAAYYAKYYLNGGDALISPFLTSGVIASVL
                     AMIACTWLTRLYDKIKIFRYTQLLAFVVGGAMYFVQPNSIVLAFTFYIVVTFLTDIQL
                     PIYWASIAESVDYGEMKTGQRVSGLAFGGILFFQKFGMGLAGGFIGIALAYLDYQPGV
                     EQTPQALWGICLLMTIFPAILNLITGLIMRFYLINNEFYEQIKARLQTAEE"
BASE COUNT         9380 a         9971 c         9433 g         8932 t
ORIGIN      
        1 cataatcagt acggggttgc tacaacatgt attacttttt caccacaatt aacggttcaa
       61 tatcactctc ttttttgatg accagtgatt catcaccgcg caaacacgtc ccaccgtagt
      121 tgccgccaac ggtgaaggta catacctgaa tgtatttacc gtccactttc ggcaaacacc
      181 acagttgctg atagatgttt ttctgctcgg caaatttacc gctggttttg tccagcacct
      241 cttcatgatg gctgacgagg tcgatattgc tgccacagcg accggcgatc ggtttcactg
      301 cgtaacctgt tttcaccagt tcatcattaa cagtgaaatc ggtatccagc aggtaacgat
      361 ggtgcgggaa cagcgaccag aggatcggca gaatcgcttt gttgccgggg atcaccgtcc
      421 acagcggctc aaagaccagc acttccgggc gcagcaatac gtcgataaga cgcacttcgt
      481 tttgcggatg accggtacgg attggcaccg cagcaaactc acggtcgcta acttcacgaa
      541 tctgatcaaa cgcggtttcc cacgcccagg ttttccacac gcagttaacc agccgtcctt
      601 ccccatcaat cagttgcccg gcagcatccc agcccagttc atccagcccg cgcaagatac
      661 gcgtttcaaa gcccgcctgg tgcagcgcct gctccataaa ctgcgcgtga tagttttcct
      721 cgatatcttt gtcctgcatg atatggacaa acggacgtgc acgactgtgt ttccaggcac
      781 cagccagttc gttaatcagc ccttccgccg gattgaagcc gttgccttta tagccctgct
      841 ccgcccaacg ttcgaggatc aagcccgctt cggtatgaca tgaggcagag tcggcgttgt
      901 attcgtaaac cttcaggcca cgctcatcca tgcagaaatc catacgacca gtgatcatat
      961 ggtgacggcg acgctgccag gagagacgca aacgtggcca gaggattttc gggatgtcga
     1021 acagcgccag caggttgtca tctttcagca ccttgtcggt tgcgtgaaga tacatcaggt
     1081 gcagctcgtt ggtggcttta attagctcct gctcggcact ctcggtaatg gtgtagtaat
     1141 gataaggatc ctgattgatc acctgaccgt tggcctgcac ataggcgttt tgcagcggat
     1201 ctttttcatc cagccattta ccgtcaaact ggcctttgtt ttccaggcgc gctccgctga
     1261 ttttcagcag ctcgcctgca atttccggct gcggtaagct gtattcagta tcttccgtct
     1321 ggatcatcca gcccagaatg gtggtgtcat caaaagtgtc tttcagggta tagcagccgt
     1381 tttcgaccac catctccagc tcacgcgtcc actgttgccc ttgcggcaac ggggaatgaa
     1441 tcacgttctg ttccgcaata cggactttgt tgccatgcaa ttgggtaatg atggcgacat
     1501 ggccagtgtc tttaaattca ccgcctttat cccagataag aagcgcaccc gcgaccggcg
     1561 cacgcggcga gccgttagga aatgcctgca atggcaggat gttgtcatta accacttctc
     1621 gcaggaagcg cagcgagaaa atctcccacg ccatacccac gtcagtaaag accacaccgt
     1681 aattcagaaa gagaaaacgg cgagcaaatt caacgcattg ccacttgtgg cccatatatt
     1741 cgtcgtcgat atagctacgg aatacggcgt catcttcgta ttcctgcgga tcgagagaac
     1801 tgtaatctga agagtagatt gctaccccac ctggggcgta gcccaataat gtcccgaacg
     1861 gggcatcctg gctggtcgtt cctttgctca tcactttacc tttaacaata caacctaagc
     1921 agttggcgta attgttgtgc tctgattacc tgctcagcga cattaaccgg acagaggtca
     1981 gactaatcat acacctcatc gcagcggctg gcgcaaaggg ttaaaaaatt cacattctgt
     2041 acagcaagtg acctgctaca ctgcttcaac actaccactc agaaggcaac tcactatgac
     2101 agacaatact tatcagcccg cgaaagtctg gacgtgggat aaatccgctg gcggcgcgtt
     2161 cgccaatatc aatcgcccgg tttctggtcc gacgcatgaa aaaacgctgc ccgttggcaa
     2221 acacccattg caactttatt cgctgggaac gccgaacggt cagaaagtaa cgattatgct
     2281 tgaggagctg ctggcgctgg gcgttactgg tgcagagtac gacgcctggc tgattcgtat
     2341 tggcgatggc gatcaattct ccagcggctt tgtcgaagtg aacccaaact cgaagatccc
     2401 ggcgctgcgc gatcatacgc ataatccgcc gatccgcgtg tttgaatctg gttcgatcct
     2461 gctttatctg gcggagaaat ttggctactt cctgccgcag gatttggcaa agcgtactga
     2521 aacgatgaac tggctgttct ggttacaggg cgcggcaccg ttcctcggcg gtggttttgg
     2581 tcacttttac cattacgcac cggtaaagat tgagtacgcc atcaaccgct ttaccatgga
     2641 agccaaacgt ctgctcgacg tgctggataa gcaactggcg cagcataagt ttgttgcggg
     2701 cgatgagtac accattgcgg atatggcgat ttggccgtgg tttggcaacg tggtgttagg
     2761 tggtgtgtat gatgccgctg agtttcttga tgcgggcagt tataagcatg tacaacgctg
     2821 ggcgaaagaa gtaggcgaac gtccggcggt gaaacgtggg cgtattgtta accgcaccaa
     2881 cggaccgctg aatgagcagt tgcatgagcg ccatgacgcc agtgatttcg agacgaatac
     2941 ggaagataag cgtcaggggt aagggttggt gttcgtcgca gcaagccatc caggccggat
     3001 aaggcgttca cgccgcatcc ggcaatcgtg cacaatgcct gatgcgacgc tgccgcgtct
     3061 tatcaggcct acgaaagcta atcatcacgc gctggtaatg tcgtaatcca tttggcgcag
     3121 tgcgtctaat gtggctttgg cttcatcttc gtcgatgatg ctcatggcaa atccgacgtg
     3181 taccagcacc cactggccca gtagatcggc agggttacct tcacaaatca gggcgatatt
     3241 cacgtcgcgc ttgataccac atacttcaac ctgcgcaagc tggtgaatat cttcaccgac
     3301 agccagcacc tggcctggaa cgccaataca cattattaac tccggttatt caacttcaat
     3361 acttttgacg atcagcgaat cgccggtatc gacacgcaac cgctcgccgt gacagagcgg
     3421 acactgcgca tcgtgctgat gaatctccac cacctggcag caatcccagc accaggcctg
     3481 ggcgggttta tagacgatat gtaaatcgca cccttgcgcc accgttccgt ggcagacaat
     3541 ttcaaaacta aaacggacgg cgctctcctc aacgcaggag agcgcgccaa tttccagcca
     3601 cacggcggtg acgcgcttaa catcgtgctg ctccgcctgc cgttggataa tttcaacggc
     3661 gctctggcaa agagacaact catgcatttt cgccactcct gcgaccaaac agcagggcgc
     3721 gacgccctgc gtgtggtaca tccggattcg tgactggcag cgaaaggatc attcgtgcgc
     3781 agtcatccgt caggcgttgg ccctcttcaa tcgacatgct gtgcgaaagc ggcgacatca
     3841 gcgaacagga gagatattgc gaaacaccgt ccagttcgcc aacggtaaag gtcatagtgc
     3901 catacggcag ttgcagacca attttttcac tcactttgcg cagcggccag agttgatccg
     3961 ggccggggaa gataactgca ctcagcatcc acggggtgat cacacacccc gtccactgac
     4021 cttcgaacag cgtaaaatca gaaacataca ccggcattga aggatgcaga aaagagagat
     4081 cgtgcatcga acgccgggca atttcttcaa acgctgcctg tacttgcgcc ttcggggagg
     4141 tctggaaacc tgctatctct tcagtcatga atcgcctccc gtgggatggc ttccacgcca
     4201 gattcacgca gcgcagccag aacctgctca agcgcaggtt caatcattgc ttcaaccgtc
     4261 ggcgttaagc cgatgtgtgg ctccagcgat tccgggatca cgccgaccag ggtgagcttt
     4321 ttcggaaact caccggtgaa gcgcagggcc gacaagacgt cggccaggcc aagctgatgc
     4381 ggagagattt tgttggtaaa caacgccgga acttcttcat cgcgcaggat catcatcgtt
     4441 cccggcgcgc tcttcttcga cacaatggca tccgcaataa tcagatgatc gcgatttgcc
     4501 atgtcgccaa gcagctccat tcccgccgtg ccgccatcga ggatctcaac ataatccggc
     4561 agaatgtatc gttgctctaa cgcttcgaca atccgcacac cgatggcttc atcggtcagc
     4621 aaaatattgc cgacccctaa gactaaaata cgcattacag aaccttcact gaaaccactt
     4681 cgttgccgtc agcatccact acgtgtaccg cacaggccat gcacgggtcg aaggagtgaa
     4741 tggtacgcac cacttccagc ggtttattcg gatcggcaac cggtgtaccc accagcgact
     4801 gctcgtaagg accgacgtca tcattgaagt tacgcggacc agagttccag gttgatggaa
     4861 caaccgcctg gtagttactg atgataccgt ctttgatcac catccagtga gagagcatac
     4921 cgcgcggcgc ttcgaggaag ccaacacctt tgaactcacc cgttgccgga atgttcggct
     4981 tcacaaaggt ggtatgatcg cctttaccga tattggtgat cagtgcactg tattgatttt
     5041 gcaggatatc ctgcaattcg cagcagtgaa cggtacgacc aataatacgg cccagcgtag
     5101 agtgcagttg cgccacttca agcgtgttac cagtcagttt ctgataaatc gcaacgattt
     5161 cattcagttt gttctgggta gactcgcgtc ctgccgccag tttcaccagc atgttagcca
     5221 gcggacccac ttctaccgtt ttgccgtaga aagtcggtga tttcacccag gaatatttcc
     5281 cgtcgtcaga ccaaccatca taagccggaa tggtggtacc ttcccacggt gcctgcggcg
     5341 cttcgtcttt ataccaggag tgcttcgcgc tttcctgaat gcctttgatc aggtattcat
     5401 cggaatgaga agtgatcgga cgatacgagg acagatccgc attctcaatg tagccgcccg
     5461 ggaacaggaa gctgccgttt ttactgtcgg tcgggaattc cggcacgctc aggtagttca
     5521 ccgcaccttt accgcgcgtc agccattccg ggtagaacgc ggcaataact gcggtatcaa
     5581 ccttataaac ctgttcaaca aagtcgctca gcttgtcgat gaaagacttg atgtacatca
     5641 ggcgctcaag gttcagcacg cccaaaccgt cgaggttgat cgggttcgcg acaccaccta
     5701 ccgccaggtt ctgaatgtgc ggcgttttac cgcccagcag cgccacgacg cggttagcgt
     5761 cacgctggca ctccagcgcc tgcaggtagt gcgctaccgc aatcaggttc acttccggcg
     5821 gcagtttcat cgccgggtga ccccagtagc cattggcgaa aatacccaac tgaccgctgg
     5881 caaccagatc tttgatcttg ttctgaactt tggtgaactc ttccggactg ttcaggtgcc
     5941 aggtcgaaac gcctttcagc atttcggagg ctttggttgg gtcagcttgc agtgcagaag
     6001 tgatgtccac ccagtccagc gccgaaagct gatagaaatg aacaatatgg tcatgcgtgg
     6061 tgtgcgcagc cagaatgatg ttacggatgt attgcgcgtt aaccggaacg tcgatattca
     6121 gcgcactttc tgccgcacga acggaagaca gcgcgtgagt ggtagtacat acgccacaga
     6181 tacgttgcac aatcatccat gcatcgcgcg gatcgcggtt tttcacgatc tcttccatgc
     6241 cgcgccacat ggtaccggaa gcccatgctt tcgaaacgac gccattttcg atttcgcaat
     6301 cgatgcgtaa atgcccctca atacgggtta ccggatcaat agtaattctc tggctcatgc
     6361 tttgctcgcc tcatgacgat tatgatcgtt ttgttttaaa ggaggaagta tcggcagtag
     6421 acgaatgagt acgatgtaag cgcaaatctc aatagccaca aaaccaatag aaatcaacag
     6481 ttcttcccag gtcgggaagt aggcgtaacc gccgcccggg ttgaatgcca ccagcgaata
     6541 ggtcagacgc caggttgcac aacctaacag tgcgctcagt gctgacagga acagcatgcg
     6601 ggaatcatta cgcagcttcg ccacacgcag aacgaccagc gggaagagca tcagcaggac
     6661 ttcaatccag aacatcacgg agtagaagtc accggcaaac gctaacgaca gcttgtcgcg
     6721 atagataagc tcgccaaagc gcagcacgat gaaaatcgcc agcaacacac tgatggtgtt
     6781 ggtcagctta acaaacagac tcttttcatc cggaccgttg ccacgcagac ccgcctgcac
     6841 cagcgaacct tcaaagatga caatcgagaa gcccatgatg aacgccgtca gcagcgagaa
     6901 cagcggcaac atttcatagc tctgccacaa cggatgcacc ttgtagcccg ccgagatcat
     6961 cagcgacccc attgaagatt ggtgcatggt cggcagcagc gcaccgagcg cgatgatgaa
     7021 gaacatcacc ttgtttagtc gctgtagcga caccttccac cccagacgtt caaacagtgc
     7081 cggagcaaac tccagtgcca tcacgccgat atagatggtc atacagaccg ccgtctcgaa
     7141 cagtaccgag ttcacgttga agtggcccgg aatgtagaag tacggcaggt tccagtagcg
     7201 acccacgtcg atagtgatcg acaagccacc cagtgagtaa ccaaacagac tcgccaacag
     7261 cgccggacgc accagcggat ggtattgccc acggttaaag acgtataccg cccacgccag
     7321 cgcccagccg ccacaggcaa agccggtgcc aatcagcagg tcaaacgcga tccacacgcc
     7381 ccacgggaag ccgccgttca ggtcagagac agagcccaga ccgaacacca gacgcttcac
     7441 aatcaggagc atacagatga cgattaacgg tccaaaaatc atgaccggtt tactgatgat
     7501 tttgccgccc agcggttgtg gatcatgact catgatcgtc tcctccgtcg tgatggtcgt
     7561 ttttggtgtt gcgacgaacc agcacggtta agcccgccag cacagccagt ggtagcatca
     7621 tgcctttata cagggtgtgt tgaatatttt cggaacgcgc accggtagaa agatcgtcca
     7681 gtttcggcag gtcgagattt tcataaggca cacccgtcag taccagaacc tgagtaccgc
     7741 cgccctcttt ctcgccgtac agatgcggat aatatttcgg caccgtatgc aggtaagtgt
     7801 cgccagattt cagcgtctga cgcggatagt ggtattcgct gccaggcttc agcgccagac
     7861 gttttttcgc ctccgccatc agctcttcac gcgtaccgaa aatcaccgcg cccgccgggc
     7921 acacttctac gcagccaggc agaccgcctt tatcgagacg ttccacacct ttctggttgc
     7981 acagctcgca cttatgcagc gcaccaaacg ggttgttgta gtcgtacttc ggcacgttgt
     8041 acggacaggc gaccatgcag taacggcagc cggtgcacac gtctttgtcg taatggacaa
     8101 tgccggtttt cggatctttt ttcagtgcag agaccgggca cacagagaca cagttcggat
     8161 cgacgcagtg catacactgt ttcttaatgt acgcgtagcc gttctcctcc tggtctttgt
     8221 tgacccctgt gccgctggtc cacacctgaa tgatgttatt ggtatacggc gacagtttgt
     8281 cgttgttcga ccaggtctgt tccccttgcg ggttacgttc agggaagttg atatcctgac
     8341 acttggtgac gcaagcctgg cagcctacgc acaaggtcga gtcgtacaac atccccagcg
     8401 atcccggaat tggcgggcgg ttttcagcag ccgcatgact gacagacggc agcgcgcccg
     8461 tcagcaatgc cccgcaggag gctgctttaa taaaattacg tctgttcacg gttattctcc
     8521 ccgtgagtca gcgttatctt tcttttgctg acgacccagt tcacgcaccg ccatcacgct
     8581 gacaccggca acgagcccaa ccacaccgcc gagcaaacca atagcgcctg cagagacgtt
     8641 gccgccctct ttagcgttaa catccggttt ctgtgaacgc ggagtctgat tttcgacgtt
     8701 ggcaagctga tggatgcctt tatggaagcc gataccttct tcgttacagc catagcatgg
     8761 gtgaccaatc gctaccggcc acacgccgcc aacatcgcag aattgcagcg ttgagcagtt
     8821 gccgtaagtt tctggccctt tacagccgag gtggtacagg caccagcctt cacggtggcc
     8881 ttcatcaccg aactctttgg caaaacgacc agcatcgaag tgcgggcgac gttcgcagtg
     8941 ttcgtgaatc agacggccat aggcgaaggt cggacgattt ttgtcatcca gtttcggcgg
     9001 tttgccgtaa gtgatgatgt gcgcaacggt cgcgaggaag ttgtgcgggt tcggcgggca
     9061 gcccggaata ttgataacgg ttttgcctgg cagaacttct tgcaggctga ctgcgccagt
     9121 tgggttaact ccagctgcgg caacaccgcc ccacgcagag caggaaccga tagcgataat
     9181 cgctgctgcg ccttctgccg ctttgcggat gtgatccaca atcggctcac cagcaaccat
     9241 gcaataaata ccgttatctt ttaatgggat ggaaccatcc acaaccaaca catactgccc
     9301 tttgtacttc tcaagtgcgt tatgtttgtt ctcttcgacc tgatgaccga aggcggcgga
     9361 aagcacttcg tgatactcca gagagatagt ctccagcacg aggttttcta ccgttggatg
     9421 cgttgcacga agcagagatt ccgtacaacc ggtgcactcc tgcgcgccaa tccagataac
     9481 tggcggacgc tgcgggttag taaccgattc ggccatctct gcagcggctt tgctacttaa
     9541 ccccatggtg gcggctaatg ctgcacaaag cttcatgaaa tcacgacggt taatgccgtg
     9601 agaatggatg agggtgttat ctccagtcat ttatagttat tccgttgcga agacctggca
     9661 tatattttgc ctcaatcgca aaatcaataa tgcgatcgat gcgccattta ccacacattt
     9721 attatggtta tcgatatcat gatactgcgg cggggcgcta aaacgaaggg aagaagcatt
     9781 agtgtaatta attaaagcaa gataatacgt atgtttgatc aattttcgag gcgataaccg
     9841 gccataaaaa agcgcaacgt agaacaggaa ttattctcac gaaagaaatc cattaccatc
     9901 tctttatcca gtccatagcg gcgcgtcaga atacccgctt cccaggcgct aagctggcga
     9961 tctccggtct tttcgtacat ccggtgtgag tagcctaata caaaaccgcg tttataatcg
    10021 gcacagaatt ttgtcacatt aaccgcactg tcggcatgcg ttgcttttaa tcccgccatt
    10081 aagcctttac caaaatggtt attcatcgtt cacctcattc gctatatatc gccatatata
    10141 gcgatatttt aggcaaagaa cgacttttgc gcacgctatt ttttcatgct tgtttaagac
    10201 gtaatccagt aagagataaa cctatcagga gagatattta tcgaaccatt tcagtgtcct
    10261 ttgccaggca agatcggcgg cagatttgtc ataacggggc gtggaatcat tatggaatcc
    10321 gtgattaacc cccggataga tatacgcctc ataaacctta ttattggctt tcaacgccgc
    10381 ctcgtaagca ggccagccct cgttgattcg ggtgtccagt tccgcgaagt ggagtagtaa
    10441 aggcgcttca atcttcgcca catcggcagt gggtgcctga cgaccataaa acggcaccgc
    10501 gcaggccagt tccggatacg cgacagccgc cgcgttcgat acgccaccgc catagcaaaa
    10561 tccggtaata cccactttgc ctgccgcttg cggatagcgt tgcataaact caattgcggc
    10621 aaagaaatca ttcatcagtt tggttggatc aacctgctgt tgcagctcac gacctttatc
    10681 atcatttccc ggataacctc caacggaact taagccgtca ggtgccaggg cgatataccc
    10741 cgccttcgcc actcgccgtg ccacatcttc gatatacgga ttcagtccac gattctcatg
    10801 caccactacc acggctggcg ttttgccgct catttttgcg ggtttcacca gataaccccg
    10861 tacctcgccg tgaccatttg gcgaaggata cgtgatgtac tcagcaacaa tctccgggtc
    10921 ggtaaactct acctgagtcg ccagcgcata atttggcttg agcaaatcaa acaacgctaa
    10981 tgccgtcatc ccgcccaccg catacttcgc cgcaaggttg aggaactcac gtttcgagat
    11041 tttcccgtga gcgtaatagt cgtagtaatc caacaactct tgtgggaaat ctttggcggt
    11101 taaacgcggc atcgttgcac tcctcaactg gtgttttttt aagcaaagca taagcacgtg
    11161 tttttgccca gtttttcgtc actctgtgag ccagactacg ggatacgcgc tggcgaatcg
    11221 ctaaactaga aacattgttt cgaaattgaa cggtggaaag gagaggtcat ggtctggtta
    11281 gcgaatcccg aacgttacgg gcagatgcaa taccgctatt gcggaaaaag cggtttacgc
    11341 ctgcccgcgt tatcgctcgg tttatggcac aatttcggtc acgttaacgc gctggaatca
    11401 cagcgtgcga tcctgcgtaa agcgtttgat ttgggcatta cgcactttga tttagccaac
    11461 aattacgggc cgcctccagg aagcgcagaa gagaactttg gtcgcctgct gcgggaggat
    11521 tttgccgctt atcgcgatga actgattatc tctaccaagg ctggctacga tatgtggccc
    11581 ggcccttacg gctctggcgg ttcacgtaaa tacctgctcg ccagcctcga ccaaagcctg
    11641 aagcgtatgg ggcttgagta tgtcgatatc ttttactctc atcgcgtcga tgaaaatacg
    11701 ccgatggaag aaaccgcctc tgcgctggct catgcggtac aaagcggtaa ggcgctgtat
    11761 gtcgggatct cctcttactc gccagagcgg acgcaaaaaa tggtcgagtt gctgcacgag
    11821 tggaaaattc cgcttttaat tcatcaacct tcgtacaatt tactgaaccg ctgggtggat
    11881 aaaagcggcc tgctggatac cctgcaaaat aacggcgtgg gctgtattgc ctttactcct
    11941 ctggctcagg gattgctgac cggaaaatat ctcaacggca ttccgcaaga ttcacggatg
    12001 tatcgtgaag ggaataaagt tcgtggtctg acaccgaaaa tgcttaccga agccaacctc
    12061 aacagcctgc gcttattgaa tgaaatggca cagcagcgtg gacaatcaat ggcgcaaatg
    12121 gcgttaagct ggttgttgaa agatgatcgc gtgacgtcgg tattgattgg tgccagccgc
    12181 gcggagcaac tagaggagaa cgtgcaggcg ctgaataatc tgacatttag caccgaggag
    12241 ctggcgcaga ttgatcagca tatcgccgat ggcgagctga atctgtggca ggcgtcgtcc
    12301 gataaatgac ctgttaataa cgggcgcgcg aaccgcgccc gaatcagatc agtgattatg
    12361 acgagtcagt cggtcaagat agcccatcac aaatgcagaa agcacaaacg tcagatggat
    12421 aatgacgtac cacatcagtt tgttatccgg gacatttttc gcatccataa agacgcgcag
    12481 taggtgaatg gaagaaattg ccacaatcga cgctgctact ttgtttttca gcgacgttgc
    12541 gtccattttc cccagccagt tcagcttctc tttgttctcg gagatatcca gctgtgagac
    12601 gaaattctca taaccggaaa acatcaccat caccagcaaa ccgccaacca gcgtcatatc
    12661 caccagcgac agcaacacga ggatcaaatc tgattccgcc atcgagaaga tattcggcag
    12721 tacgtgaata atctcctgga agaacttcag cgccagggca actaacgcca gcgaaaggcc
    12781 aaagtacacg ggggcaagca gccagcgaga agcatacatt gcattttcaa gaaaacgttc
    12841 catacattcc tgtcgtgtag ttaaaacggg caacagtata tcgcaattga gtaaacagca
    12901 cgcaacagca aatcaacata tacgttaaac ctttgtcgca taattctgag gtttagggat
    12961 cttcctggtt cccttatcaa cgcatcgaga gcgtctaccc ttaaaggacg ttgaacctga
    13021 agggagaaaa acgatgtctc atctaaaaga cccgaccacg cagtattaca ctggtgaata
    13081 tcccaaacag aaacaaccga cgccaggcat ccaggcgaag atgacaccgg taccggattg
    13141 cggcgagaaa acctatgttg gtagcggtcg cctgaaagat cgtaaagcac tggtgacagg
    13201 gggcgattcc ggaataggtc gcgctgccgc catcgcttac gcgcgtgaag gggctgacgt
    13261 ggcgatcagt tatcttcccg tggaagaaga agacgctcag gatgtgaaaa agatcattga
    13321 agaatgcggt cgcaaagccg ttctgctgcc aggcgattta agcgatgaga aattcgcccg
    13381 ttcgctggtt cacgaagcgc acaaggcgtt aggcgggctg gatattatgg cgctggtcgc
    13441 cgggaaacag gttgccattc ctgatattgc agacctcacc agcgaacagt ttcaaaagac
    13501 ctttgccatt aacgttttcg cgctgttctg gctaacccag gaagcgatcc ccctgctacc
    13561 gaaaggtgca agtatcatca ccacttcgtc aatccaggca taccagccaa gtccgcattt
    13621 actggactat gcggctacga aggcggcgat tctgaactac agccgtggcc tggcaaaaca
    13681 ggtcgcggag aaaggtattc gggtgaatat tgtcgcgcca ggccctatct ggacggcact
    13741 gcaaatttct ggcggacaaa cgcaggataa gatcccgcag tttggtcagc aaacgccgat
    13801 gaaacgtgcg gggcaaccgg cggaactggc ccctgtatat gtttatctgg caagtcagga
    13861 gtcgagctac gtcaccgcag aagtgcacgg tgtgtgcggc ggcgagcatt taggttaaaa
    13921 aaatgcccgg ttgtgaaaag caaccgggca tcattgtgaa ttcactctta cctgatgtgg
    13981 cgaaaacgcc ttatccgcct acaaacgcat gaagattcaa catgttgcaa tagattttgt
    14041 aggcctgata agcgtagcgc atcaggcatt gagcacctaa tgccggatgc ggcgtgaacg
    14101 ccttatccgg cctacaaacg cacgaagatt caacatgttg caatagattt tgtaggcctg
    14161 atgagcgtag cgcatcaggc aattctactt tacttcgctt tggcggtttc ttcgccgacc
    14221 agacctatct tcaggtaacc cgcctgatgc agcgtatcca ttaccttcat caacgtctcg
    14281 taatcgacgg ttttatccgc tcggaagaag atggtggtgt ctttcttgcc ttcggttaac
    14341 gcattcaacg ccgtaatcat tgtttcatcg gtgaccggat cgttaccgat aaacatcgag
    14401 ttgtctgcct tcaccgacag ataaaccggt ttttccgggc gcggctgcgg cgtgctggta
    14461 gaagcaggca agttcacctt cacatctacc gtcgctaacg gtgccgccac cataaagata
    14521 atcagcagaa ccaacatcac gtcgataaac ggcgtcacgt tgatatcatg catttcgccg
    14581 ttatcgtcga ggttttcgtt aagatgcatt gccatcggat attatcctgc gcgtaatttt
    14641 tgtgcgacac gaaccggatg cgcagcggcg ctggcttcca gatccaggtc acggctttgc
    14701 agcaacaata cctgcgctgc aacatcaccc agcatcgctt taaagccgcc aatctggcgt
    14761 gcaaatacgt tatagataac gaccgcagga atcgctgcca cgaggccgat tgccgttgct
    14821 aacagagctt ctgcgatacc cggcgcaacg actgccaggt tagtggtctg cgtttgcgcg
    14881 ataccaataa agctgttcat gatgccccag accgtaccaa acagaccaac gaacggagaa
    14941 atcgcgccga tggttgccag atagccgtta ccgcggccca tttgacgacc cactgcggcg
    15001 acccgacgct ccagacggaa gctggtacgt tctttaatac cttcgttatc gtcgctgcct
    15061 tctgacagtt ccagctcatt ctgcgcttca ttgagcaaat gcaggcttaa gcttttgcta
    15121 ccaaaatcag cggcgatatc gttggcctgg tttaaggaac gcgcttcagc cagcagttgc
    15181 tgctcgcgct taaggcgacg cttctgattg aagaactcta cgctcttact gaagaagatt
    15241 gcccaggtga ctacggaggc caaaataagc ccaatcatca cgcacttaac gacaatatcg
    15301 gcgtgctgat acatacccca gacggaaagg tccgtctgca ttaaattatt acccactgtg
    15361 tatctccagg acgcaagtca caaaatctgc gcataataat atcaaaacga cgtcgaattg
    15421 atagtcgttc tcattactat ttgcatactg ccgtaccttt gctttctttt ccttgcgttt
    15481 acgcagtaaa aaagtcacca gcacgccatt tgcgaaaatt ttctgcttta tgccaattct
    15541 tcaggatgcg cccgcgaata ttcatgctag tttagacatc cagacgtata aaaataggaa
    15601 tcccgacatg gcggacaaaa agcttgatac tcaactggtg aatgcaggac gcagcaaaaa
    15661 atacactctt ggcgcggtaa atagcgtgat tcagcgcgct tcttcgctgg tatttgagag
    15721 tatggaagcc aaaaagcacg cgacgcgcaa tcgcgccaat ggcgagttgt tctatggacg
    15781 gcgcggaacg ttaacccatt tttccttaca acaagcgatg tgtgaactgg aaggtggcgc
    15841 aggctgcgcg ctatttccct gcggggcggc agcggttgct aattcgattc ttgcttttgt
    15901 cgaacagggc gatcatgtgt tgatgaccaa caccgcctat gaaccgagtc aggatttctg
    15961 tagcaaaatc ctcagcaaac tgggcgtaac gacatcgtgg tttgatccgc tgattggtgc
    16021 cgatatcgtt aagcatctgc agccaaacac taaaatcgtg tttctggaat cgccaggctc
    16081 catcaccatg gaagtccacg acgttccggc gattgttgcc gccgtacgca gtgtggtgcc
    16141 ggatgccatc attatgatcg acaacacctg ggcagccggt gtgctgttta aggcgctgga
    16201 ttttggcatc gatgtttcta ttcaagccgc caccaaatat ctggttgggc attcagatgc
    16261 gatgattggc actgccgtgt gcaatgcccg ttgctgggag cagctacggg aaaacgccta
    16321 tctgatgggc cagatggtcg atgccgatac cgcctatata accagccgtg gcctgcgcac
    16381 attaggtgtg cgtttgcgtc aacatcatga aagcagtctg aaagtggctg aatggctggc
    16441 agaacatccg caagtagcgc gagttaacca ccctgctctg cctggcagta aaggacacga
    16501 attctggaaa cgagacttta caggtagcag cgggctattt tcctttgtgc ttaagaaaaa
    16561 actcagtaat gaagagctgg cgaactatct ggataacttc agtttattca gcatggccta
    16621 ctcgtggggc gggtatgaat cgttgatcct ggcgaatcaa ccagaacata tcgccgccat
    16681 tcgcccacaa ggcgagatcg attttagcgg gaccttgatt cgcctgcata ttggtctgga
    16741 agatgtcgac gatctgattg ccgatctgga cgccggtttt gcgcgaattg tataacattg
    16801 ccacttttgg acaattttgc agacatttaa ttgtgaaaag tcttaaattg ttgcgtccgg
    16861 gatcaaggcg tcccggacga atcaggagta caataggcag ataaaggctt aaacgctgtt
    16921 ccacaggaaa gtccatggct gttattcaag atatcatcgc tgcgctctgg caacacgact
    16981 ttgccgcgct ggcggatcct catattgtta gcgttgttta ctttgtcatg tttgccacgc
    17041 tgtttttaga aaacggcctg ctgcccgcct catttttgcc aggcgacagc ttgttgatac
    17101 tggcaggcgc attgattgcc caaggggtta tggattttct gcctacgatt gcgattctga
    17161 ccgccgcagc aagtctgggc tgctggctaa gttatattca ggggcgctgg ttagggaata
    17221 ccaaaacggt gaaaggctgg ctggcacagc ttcctgctaa atatcaccag cgcgccacct
    17281 gcatgtttga ccgccacggt ctgctggcgc tgctggctgg acgttttctt gcatttgtcc
    17341 gtacgctgct gccaaccatg gcgggaattt ccggtctgcc aaaccgccgc ttccagtttt
    17401 tcaactggtt aagtggattg ctgtgggtca gcgtggtaac cagttttggc tatgccttaa
    17461 gtatgattcc gttcgttaaa cgccatgaag atcaggtaat gacgttcctg atgatcctgc
    17521 caattgcctt gttaaccgct ggcttgttag gcacgctgtt tgtggtgatt aaaaaaaaat
    17581 actgtaacgc ctgacgattt tccccgttcc cggctgctgt accgggaacg tatttaattc
    17641 ccctgcatcg cccgcattct tgccgcatct tcccccggcg tcacaccgaa gtaacgttta
    17701 aactcacggc taaattgcga tgcgctttca tagccgacgc gcatcgctgc tgcgctggcc
    17761 ttcatgccgt catggatgat catcatccgc gccttatgca gacggtaatt cttcaaatac
    17821 tgcaacggcg aggtgctggt gacagactta aaattatggt ggaacgccga tacgctcatg
    17881 ttggcttctg ccgccagttg ctcgacgctc aggttttcgg tgtatttatt ctcaatccgt
    17941 ttcagcacgc ggctaatcag actgaagtga gtctggcgac tgaccagcgc cagtaacgcg
    18001 ccgccgcaag gtccggtcag cacgtagtac agaatttcgc ggatgatctg tttgccgaga
    18061 atacgcgcat ccagtggtcg ctccatcacg tcgagtaacc gctccgccgc gcataaaatc
    18121 tcttctgata acgtggcgga gttaatcccg ctggctgcca tcgacggctg gaaatgctca
    18181 tcttcgccaa tgtccatcaa cagttcctgt aactgcaaaa tatcgacatt gagacgcaac
    18241 cctgccagcg gcacctctga cgtggcatag gtttcgcact caaacggcaa cggcaccgtc
    18301 agcagcaggt attcattggc atcataacga aacacgcgtt cattgatata accgatttta
    18361 tgcccggaaa agagaattat gatgccaggc tcgtacatca ccggtgtacg tgcgaaaggc
    18421 gtctcgccat acaacaaacg cacatcgggc aacagttctg acaaactatt ttctttaatt
    18481 ttcagtttat taactttatc cgccagcaag cggcaaatct cttcacgttt catatcgcgt
    18541 aatttcttag gaataatgcg gcaatttgat tgtgcgcaat tttgtagcat ttctccagca
    18601 ctctggagga ataggcaaga cattggcaga aatgagcatt gagagccagg gcgctggcga
    18661 tcacaatgaa aaacatcagg cagatcgttc tctgccctca tattggccca gcaaagggag
    18721 caagtaatga acaactttaa tctgcacacc ccaacccgca ttctgtttgg taaaggcgca
    18781 atcgctggtt tacgcgaaca aattcctcac gatgctcgcg tattgattac ctacggtggc
    18841 ggcagcgtga aaaaaaccgg cgttctcgat caagttctgg atgccctgaa aggcatggac
    18901 gtgctggaat ttggcggtat tgagccaaac ccggcttatg aaacgctgat gaacgccgtg
    18961 aaactggttc gcgaacagaa agtgactttc ctgctggcgg ttggcggcgg ttctgtactg
    19021 gacggcacca aatttatcgc cgcagcggct aactatccgg aaaatatcga tccgtggcac
    19081 attctgcaaa cgggcggtaa agagattaaa agcgccatcc cgatgggctg tgtgctgacg
    19141 ctgccagcaa ccggttcaga atccaacgca ggcgcggtga tctcccgtaa aaccacaggc
    19201 gacaagcagg cgttccattc tgcccatgtt cagccggtat ttgccgtgct cgatccggtt
    19261 tatacctaca ccctgccgcc gcgtcaggtg gctaacggcg tagtggacgc ctttgtacac
    19321 accgtggaac agtatgttac caaaccggtt gatgccaaaa ttcaggaccg tttcgcagaa
    19381 ggcattttgc tgacgctgat cgaagatggt ccgaaagccc tgaaagagcc agaaaactac
    19441 gatgtgcgcg ccaacgtcat gtggggggcg acgcaggcgc tgaacggttt gattggcgct
    19501 ggcgtaccgc aggactgggc aacgcatatg ctgggccacg aactgactgc gatgcacggt
    19561 ctggatcacg cgcaaacact ggctatcgtc ctgcctgcac tgtggaatga aaaacgcgag
    19621 accaagcgcg ctaagctgct gcaatatgct gaacgcgtct ggaacatcac tgaaggttcc
    19681 gatgatgagc gtattgacgc cgcgattgcc gcaacccgca atttctttga gcaattaggc
    19741 gtgccgaccc acctctccga ctacggtctg gacggcagct ccatcccggc tttgctgaaa
    19801 aaactggaag agcacggcat gacccaactg ggcgaaaatc atgacattac gttggatgtc
    19861 agccgccgta tatacgaagc cgcccgctaa gctttttacg cctcaaactt tcgttttcgg
    19921 gcatttcgtc cagacttaag ttcacaacac ctcaccggag cctgctccgg tgagttcata
    19981 taaaggagga acgtatggct aatccaaccg ttattaagct acaggatggc aatgtcatgc
    20041 cccagctggg actgggcgtc tggcaagcaa gtaatgagga agtaatcacc gccattcaaa
    20101 aagcgttaga agtgggttat cgctcgattg ataccgccgc ggcctacaag aacgaagaag
    20161 gtgtcggcaa agccctgaaa aatgcctcag tcaacagaga agaactgttc atcaccacta
    20221 agctgtggaa cgacgaccac aagcgccccc gcgaagccct gctcgacagc ctgaaaaaac
    20281 tccagcttga ttatatcgac ctctacttaa tgcactggcc cgttcccgct atcgaccatt
    20341 atgtcgaagc atggaaaggc atgatcgaat tgcaaaaaga gggattaatc aaaagcatcg
    20401 gcgtgtgcaa cttccagatc catcacctgc aacgcctgat tgatgaaact ggcgtgacgc
    20461 ctgtgataaa ccagatcgaa cttcatccgc tgatgcaaca acgccagcta cacgcctgga
    20521 acgcgacaca caaaatccag accgaatcct ggagcccatt agcgcaagga gggaaaggcg
    20581 ttttcgatca gaaagtcatt cgcgatctgg cagataaata cggcaaaacc ccggcgcaga
    20641 ttgttatccg ctggcatctg gatagcggcc tggtggtgat cccgaaatcg gtcacacctt
    20701 cacgtattgc cgaaaacttt gatgtctggg atttccgtct cgacaaagac gaactcggcg
    20761 aaattgcaaa actcgatcag ggcaagcgtc tcggtcccga tcctgaccag ttcggcggct
    20821 aacatgcaaa ttctcccggt ggcggtaatg ttccgctacc ggacttttca gaaatcattt
    20881 attcccctcg cgtcccgccc gttgttactc ttccttgttc aggaatgcca aatataagga
    20941 catcatcatg cagagccgga agctcttaaa agaacaactc atctatatcc gggataaacg
    21001 caacggagag gtgaaaaaca gatgaaaata atacttctgt ttttagcagc cctggcaagt
    21061 tttaccgtac acgcacagcc cccctcactg accgtagaac aaacagtccg gcatatttat
    21121 cagaactata aatcagatgc cactgcccct tattttggtg aaaccggaga gcgggcgata
    21181 acttctgcgc gtattcaaca ggcgcttacc ctgaacgaca atcttacgct gccgggcaat
    21241 attggctggc tggattatga tccggtttgt gattgtcagg attttggcga tctggtgcta
    21301 gaaagcgttg cgataaccca aactgacgcc gatcatgccg atgccgttgt gcgctttcgt
    21361 atctttaaag atgataaaga aaagaccacg cagacactga aaatggtggc ggaaaatggt
    21421 cgttgggtca ttgacgatat tgtcagcaat catggcagcg tcttacaagc agttaatagc
    21481 gagaatgaaa aaacgctggc cgctttagct tcgttgcaaa aagaacagcc ggaagccttt
    21541 gttgccgaac tctttgaaca tattgctgat tatagctggc cgtggacgtg ggtggtttcc
    21601 gactcttacc gccaggcggt taatgccttc tataaaacca ccttcaagac ggccaataat
    21661 cccgatgaag atatgcaaat agaacggcaa tttatttacg acaatccgat ctgttttggc
    21721 gaagagtcgc tattttcacg cgttgatgaa attcgagtcc tggagaaaac cgccgattcc
    21781 gcccgcattc atgttcgttt tacgctgacc aatggcaaca acgaagagca agaactggtt
    21841 ttacagcggc gcgaaggcaa gtgggaaatc gctgatttta tccgcccgaa cagcggcagc
    21901 ctacttaagc agattgaggc aaaaaccgcc gccagattaa agcaatgagc tgaattaaat
    21961 aacaattagc cggaacaata aataaaaggg aacactatat gaaaacgatt ttcaccgtgg
    22021 gagctgttgt tctggcaacc tgcttgctca gtggctgcgt caatgagcaa aaggtcaatc
    22081 agctggcgag caatgtgcaa acattaaatg ccaaaatcgc ccggcttgag caggatatga
    22141 aagcactacg cccacaaatc tatgctgcca aatccgaagc taacagagcc aatacgcgtc
    22201 ttgatgctca ggactatttt gattgcctgc gctgcttgcg tatgtacgca gaatgataaa
    22261 aaaatccccg gcagcatgtc agttgccggg gatttttttt aacgtccaac cgccgcttta
    22321 gggcgtttct tcgcaccagc attcaccgga cgagattgcg tagacgacgc tttttttgcc
    22381 gtagcaggcg tctgacgctg ggtcgccatc ggcgtatgtt tcgtcaacgc cggacgggta
    22441 ttgcggttct ggcgacgagc ttcacgcatc tcttcaatgg ttggcgcagg cactaagcaa
    22501 tcgcgacggc tgccaatcag atgctttttg cccatcgctt ccagcgcctg gcggattaac
    22561 ggccagtttg ccggatcgtg gtaacgcaac aacgctttat gcaaacgacg ctgcttgtcg
    22621 cccttcggta cgaagacgtc ttcactctta taaccaatct tcgccagcgg gtttttcccg
    22681 gtgtaataca tggtggttga gttcgccagc ggcgatgggt agaagttctg tacctggtcg
    22741 agacggaaac gatgcttttt cagccacagc gccagattca ccatatcttc atcacgcgta
    22801 ccggggtgcg cggagatgaa ataggggatc agatactgct ctttacctgc ctgttttgag
    22861 taagtatcga acagctcttt aaagcggtca tagctgccca tgcccggctt catcatcttc
    22921 gataacggcc cttcttcggt atgttccggg gcaatcttca gataaccgcc gacgtgatgg
    22981 gtcgccaact ctttgatata gcgcggatct tccacggcga tgtcataacg tacgcctgag
    23041 gcgatgagga tttttttaat gcctttcaga tcacgcgcac ggcgatagag gttgatcgtc
    23101 ggttcgtggt tagtgtccat gtgcggacaa atatccggat aaacgcacga caaacggcga
    23161 caagtttgtt cagcgcgtgg cgatttgcag cgcaacatat acatgttggc agttggccca
    23221 ccgagatcgg aaatcacgcc cgtaaaacct ggaacggtgt cgcggatcgc ttcgatctca
    23281 ttaatgatcg aatcttccga acggctctga ataatgcgcc cttcgtgctc ggtaatagaa
    23341 cagaaagaac agccgccaaa gcagccgcgc ataatgttga ccgaaaaacg gatcatttcg
    23401 taagccggaa tacgggcatt gccataggcc ggatgtggca cgcgcttgta cggcagcgca
    23461 aaaacgctgt ccatctcttc ggtagaaagc gggatagcag gcgggttgat ccacacatag
    23521 cggtcaccgt gtttttgcat caatgcgcgg gcgcagcctg ggttggtttc gtggtgcaga
    23581 atgcgcgaag catgggcgta cagcacttta tcgcccttca ctttctcgaa agaaggcagc
    23641 aacacgtagg ttttttccca cggtttcggg cgcggtggct gcacggttac ggctttggct
    23701 tcctgctttt tcggtgccac cggtttgtta tccgcgcacg gcaaatcttc accatacgga
    23761 tgcgggattg ggtcgatttt tccaggggta tcaagacggg tggaatccac gccgctccag
    23821 ccaggcagcg cctctttcac gataatcgcg gtattacgca catcgcggat ttcactaatc
    23881 ggctcgccca tcgccagacg gtgcgccacc tccaccagcg gacgctcacc gttaccaaac
    23941 atcagcatgt cggctttcga atccaccagc acggaacggc gcacggtatc ggaccagtaa
    24001 tcataatgtg cggtacggcg cagactagcc tcaataccgc cgaggatcac cggtacatct
    24061 ttccacgcct ctttacaacg ctgggtataa accagtgtgg cgcgatccgg gcgcttaccc
    24121 gcgacgttat ccggcgtgta ggcatcgtca tgacgtaaac ggcgatcggc ggtataacgg
    24181 ttgatcatcg aatccatgtt gccagcagta acaccgaaaa acagattcgg tttacccaga
    24241 cgcatgaaat cgtctttgct gctccagtct ggctgggcga tgatcccgac gcgaaagccc
    24301 tgtgcttcca gcatacgacc gcaaatcgcc atcccgaagc ttgggtgatc gacatacgcg
    24361 tcgccagtaa ccaaaatgat gtcgcagcta tcccagccaa gttgatccat ctcttcacga
    24421 gacatcggca aaaacggtgc cggtccaaaa caggcggccc agtactgcgg ccaggagaac
    24481 aggtcgcgat ccggttggat cagggagata gagctcattt tgcttccaga aatgataaaa
    24541 aaataatcaa aggccgggga ttataagccg gaacgaaaga gaaatcgaaa ggtattccat
    24601 actcgccctc ctcgggcgag tatgaagatt acggtaccgg attgaccaaa agttgcccta
    24661 tcgagccacg gtccgccatt tccagcgtct gactgttgaa gtagaacggg aagtgcgccc
    24721 aggaaggctg accgaaataa acaagcagct ccacttgtcc gtcaacccaa acggtatctt
    24781 tccagcctct gtcttccggg aacggcatcg cgccattcac gttacggatc tggaacatta
    24841 cgccttcaat atggaacgct tgcggctcgt ccgcgcgtac cgtccagcgt tcccacgttc
    24901 cttgctgcgc ggtgacatca atacggttga cgtcccacag ttgtccatta atacccgggt
    24961 catcacccag actgatatcg cgactgcgaa tcggcgaacc ggccatgatt tcagttggca
    25021 gcaagcgcat cggaagactg tctgtgacca gcggcagaag gccggttggg cgcagcgtta
    25081 gcaccagggt agaaaccaga atgctggatg gctcaaagaa gccacgaata cgatcaacaa
    25141 tgctcgccgc ttcgccacag gtgatcgaca cttcatcgcc gttgctcata tccaccagaa
    25201 tctcgcggcg ctcgcccggt gccagcgaaa gttgcttcac cgatacagga gcaggcagga
    25261 atccctgatc gccagaaatc acatgtaacg ggcgaccatc gctcatctgt agttgataac
    25321 gacgagagtt cgacgcgttc agcaggcgca agcgcaccca gccacgcgag acttcaacgt
    25381 acgggctttg tacaccgtta accagcagcg tatcaccaac aaagccgccg cttcccggtt
    25441 cgttgtattc tggcgtacca aagttatcca gccgtttatc ctgaataatg accggaaaat
    25501 catccacacc ataatggttg gggataggca gcgacttgct gacttcatct tccaccagcc
    25561 acattccggc aaggccgtta tagacctgct gagccgtgcg gttgggagta ttggcgtgat
    25621 accacagagt agctgcgttc tggcgaatag gcagtacggg tgcccagtca gcgtttggcg
    25681 acatcatccg tgccggaccg cccatcagcg ggcctggtac ctgtagcccg gctaccgtca
    25741 ttgagacatt ttctgtcagg cggttgctgt aaataagctt aacatcgtcg cccttccaga
    25801 cgcggatagt cggccccagg taacgaccat tgattcccca gaccgacgcg cgtgtccctg
    25861 gcgtaaatga ccagtgcgca cgttgtacag tcataaacag cggttgccca cggcgagatt
    25921 caagcagcgg cggaacgggt agcggttgct gttgcccggc tgcgctggcc ttcaggggaa
    25981 cagcgcctgc acaaagtgca atccccgatg cctgaatgaa ctgacgccga ctgagtgaca
    26041 tatttgctcc atgtaaaact ggctaaagta ataccgtgca ggaaagtgtt ccccgcaaaa
    26101 atacagattg ccgacttaaa cttttccggc ggcttcgcgt tctgcgactt ctttatcgag
    26161 ctcggcgatt ttttgttcca ttatcgaacg acaatgggca gccagctcac gaacctgatc
    26221 tttgccatac tgactgacgt caattggcgg cagcatttcg acaatcacca gaccgttgtg
    26281 cagtcgatta agattaatct tattcgaagt tgtagagacg cacacgggaa taatcgggac
    26341 gcccgccgca attgccgcgt gaaatgctcc agtcttgaac ggtagcaggc cgcgaccacg
    26401 gctgcgggtt ccttccggga acatccagat ggaaatacgg cgttttttga aatgattcac
    26461 gacttccgca atggtgccgt gagctttagt gcgattgttt ctgtcgatca ataagttgcc
    26521 ggttaaccag tacaactgcc cgaagaaggg gatccacagc aagctctttt tacctaccgt
    26581 caccgtcggc ggttgcacaa tgttcgatgc tgtcaccatg tcatagttgt tctggtggtt
    26641 agcgatatag atagcattgc cgtagctttc agcatctgca ggtttgcggc actcaacttt
    26701 caggccgaac agcggcgcaa gacggccaaa catatgccca aaggtggcca catgtttcgg
    26761 gttacgcggg ctgaaaaggc agtaaatgga gccgaataca cagactaaga tgctgtaaat
    26821 cacggtaata ataagacgaa agatatatag cataacaccc tctgaaggtc tgagagactg
    26881 gcattgtacg tcacctgaaa tcaggttagg gcattgttaa aagccgctct ctggaaaagc
    26941 gtattgttaa tcgcaacgca tgaataaaca acggtttcaa gggaattttt atcgtttccg
    27001 ggggttgaat acgctgccgg atgacgactt aacgtttcat ccggcgttcc ttgcaagcgg
    27061 gaggaaacag cgccctcccc ggcatattac tcttcgctat caccgctgct ggcacggcga
    27121 ggagagtcga tctcaacacg atcgatacgc tgcaaaccgc gcatcaacgt accgcggcgt
    27181 ccacgttcgc cagtgacttt ctgtagctct tccggacgca gtttaatttt gcgtttccca
    27241 acatgaatgg tcagcgtgct ttgcggcggc agaacgtaca attgcgccag accatcttct
    27301 ccacgcgcgg cttctgccga tggaatgttg ataatcttgt tgcctttgcc cttcgacagc
    27361 tgcggcagat cgcttaccgg gaacatcaac atacggcctg cctgagtgat tgccagcagc
    27421 atatcggaag catcttcaat caccaccggc ggcataacat gggcattttc cggtaaggtg
    27481 atcaaagcct tacctgcacg gttacgcgcc accagatcgt taaaggtgca gacgaaaccg
    27541 taacccgcat cggaagccat cagcagtttc tgatcgtcgc tttccatcag catatggtca
    27601 acggtcgccc caggcggcaa cgttaatttg ccggtgagcg gctcgccctg accacgcgcc
    27661 gacggcagcg taatcgggtc aatggcatag ctacgaccgg tggaatcaac aaacactacc
    27721 ggttggttgc tcttaccttt caccgccgct ttgaagctat cacccgcttt ataattcagg
    27781 cccggcgcgt cgatatcatg gcctttagcg ctgcgtaccc agcccatctg cgacagcaca
    27841 atggtgacag gttcagacgg cagcatgtcg tgctcgctca tcgctttcgc ttcttcgcgt
    27901 tcctgcaacg gcgaacgacg atcgtcaccg taggcttgcg cgtctgcctg cagttctttc
    27961 ttcagcaggt tattcatttt acgctcggaa gccaaaatgc cctgcaactg gtcgcgctct
    28021 ttttccagtt cactctgctc accgcgaatc ttcatctctt ccagtttggc aagatgacgc
    28081 agtttcagtt cgaggatcgc ttccgcctgg gtttccgtaa ggccaaaccg cgacatcagc
    28141 gccggtttcg gttcatcttc attacgaatg atctcaatca cttcgtcgat attgagaaac
    28201 gccaccagca aaccttcgag gatatgcagg cgcttgagga ctttctccag acgatagttc
    28261 agtcggcggc gcacggtatc gcggcggaac accagccatt cggagaggat ttccagcagg
    28321 tttttcaccg ccggacgacc atccagaccg atcatattaa ggttaatacg atagctcttt
    28381 tccagatcgg tggtagcgaa gaggtggttc atcacctgat ccatatccac gcggttggaa
    28441 cgcggcacaa tcaccaggcg ggtcgggttc tcgtggtcag attcatcgcg cagatcgtca
    28501 accatcggca gctttttgtt gcgcatttgc gcagcaattt gctccagtac gcgcgcacct
    28561 gaaacctgat gcggcaatgc gctgataacc accgcgccat cttctttctt ccacaccgcg
    28621 cgcatacgca ctgaaccacg tccgttctcg tagattttac ggatctcggc gcgcgaagtg
    28681 ataatttccg cttcagtcgg ataatccggc ccctgcacga tatccagcag ctgatcgagc
    28741 gtggttttcg gctggtcgat taatgcgatt gccgcctgag ccacttcacg caggttatgc
    28801 ggtggaatat cggtcgccat gccgacggca ataccggtgg tgccgttaag caaaatgttt
    28861 ggcagacggg caggtagcat tttcggctcc tgcaaagtgc cgtcgaagtt tggtacccag
    28921 tcagccgtcc cctgccccag ctcgctcaat agcagctcgg aatatttcga caaccgggat
    28981 tcggtgtaac gcattgccgc gaacgatttc ggatcgtccg gcgcgcccca gttcccctga
    29041 ccatcaacca gcggataacg gtaagagaac ggctgcgcca tcaggaccat cgcttcataa
    29101 caggcgctat cgccgtgcgg atggtattta cccagtacgt caccgacggt acgggccgat
    29161 tttttaaatt tggcgctggc attcaggccc agttcagaca tcgcatacac aatgcggcgc
    29221 tgaacaggtt tcagaccatc accaataaac ggcaacgcac ggtccatgat cacgtacatg
    29281 gagtagttta agtaggcgtt ttccgtaaat tcatgtagcg caaggcgctc tgccatatcg
    29341 ctcattaatt ctgattcctc aacttattcg ccagccttca aaggggcaat attgccggag
    29401 atactacatt atctgccgcg tcgagtcaca ggagatcagc gtgaatagcg ccgccggacg
    29461 tgctccgacg gcgataaagg catcaatgtg ccttgatata caactctttg ctgtagacat
    29521 aatcctgggg attatgcagc ggaaaaccgc caacgtaggg tttaagcagt ttgattaacg
    29581 gctgatagta gataggaatc agcggtgtct gttggttgat gatcacttct gcctgctgat
    29641 ataacgcatt acgctttgtc gcatcagtga tctgcgtggc ctggtttagt aaggcgtcat
    29701 actgcgcatt tttccagtga ccgacgtttt cttcactatc gcttttgagc gtgttcagga
    29761 agctggaagc atcattgtac gtcgcatccc acgactgccg agacagcatg aaatcaccgg
    29821 ctcgtcgggc atcaagatag gttttccact ccattgtgcg cagcgtcacc tgtgcaccca
    29881 gccatttttt ccattcggaa gacaacgcta tcgcggtctt ttcatgcaga tcgtacttgt
    29941 tgtagaacag ctcaaagcgt agcggatgag aggcgtcgta tcccgcctgt ttcagcaagg
    30001 cttttgccat cgcgacgcgc tcactcattg gcttttgcag ttcatcgaac gtcgtcgcgc
    30061 taaagccttt tacctctggc ggcgtcagcg tggttgcggg cgttctcaac cccagtacct
    30121 tttgcgcaat aagctgtcga tcaaccgtaa gatatagcgc ccgacgcact cgcacatcgt
    30181 taaatggcgg tttctcaagg ttgaagttgt aatattcgct gttcagacgc ggaataattc
    30241 gtagctcgcc aggcagtgat ttttcaatgg cgggaatttg ctgcgccgga acccaggtga
    30301 gatcgacctc tcccgcgcga tagcggttat agccggtgac cgaattatct agcgccagat
    30361 actcaacctg ttgcaatact gtatgttgcg catcgcggta ctttggattt ttgcgtgcag
    30421 taatcttttc gttaactacc cactgatcaa gcacaaaggc accgttgtaa accatgttct
    30481 ctggcttact ccagctatcg ccatgtttag cgatgacatg atgaggaacc gggaacagcg
    30541 tcggccaggc gagcatcgtc gtgaaccaag gaaccggctg ctcaagcgta acttcaagag
    30601 tacgatcatc cgtcgctttg acacccagcg atgtaacatc tgctttaccc gcaacaatag
    30661 ctgcggcatt gttaatgtgc gcctgtgcca gatagccagc aaaagggctt gccgttttcg
    30721 ggtcaaccgc gcgctgccag ccgaggacaa aatcctctgc cgtcagaggc tgaccgtcgg
    30781 accactgcaa accgctacgc agatggaaaa tatagcgctt gccgccgtcc agtatctccc
    30841 agcgttcagc ctgagcgggc tgcacctggc cttcaccgtc catccatacc agaccttcaa
    30901 acagatccag cacaatctgc gcggcagtat tctcctccac cttttgcggg tcgagcgtac
    30961 ctgggtcgct atgattgttg taacgaaaga cttgttgcgg ggcgagcggt gtgttggcgg
    31021 gaacgtcagc agcgtagaga ggagccgcac ttaccaggct gaccagccat aataaatttc
    31081 gcgtatacat ggtattcctt accggagaaa ttataagccc aacaacaata cgcctgtggc
    31141 attgttcgct caaacttatc gcgagtgatt tggctcacac tccggtaaag aaaaaagcga
    31201 gttgcccccg cttttccatt aattaacgga tttcattcaa tagttctgga tgcttatcca
    31261 gaacacgtaa aagtttgatt gtggaaggat gaggttgggc attgcctttt tcgtaacgcg
    31321 aaaacgcatt tacacctccc ccaaaaattt cgcttgcctc tttttgggta agagagagct
    31381 tttttcgaac cttcactata aattcaggtg ccactgtttc ggcattcacc gaagcccgaa
    31441 atgcctttac ttgcgccatg aaagcatctg actcttcttt attcatgatg ctctcttcgc
    31501 aatggacaca atataaaccg tggatacctt tcaatactgt ttttcgtcca cggaaggtgt
    31561 atggaatatc tttaatgcca gaaaccattt ctccctggtg gcaaaccgga catttcatat
    31621 tacttctcct taaacgagac gatcagtacg tcatgaatta ccgtaatttt aagataaacc
    31681 tggcctgtaa caagcctggg tctgtaaaca tcctgccaga tagtatgatc agagtaggtg
    31741 gtcatgcttt tataaaagtc gctctctgat aatccaatga taacattaca cataccatca
    31801 aaatccaaac ctaactcatc tgcatttaac agtgcactac gtgttgtacg aacttgcccg
    31861 gcattgacaa gttttttaac ctgactcaaa cgtgtatgtg gtgtgcgttt ttccataacc
    31921 cccgcctccc tgttacttta gttataacct aaaaggttaa ttacagcaat gaaaaaccac
    31981 ctaaaaggtt agttagatgt acggatatag tgaccacaca aaacgtattc tttaaggaaa
    32041 gtgattgacc atataagaaa gtggcgcatt agtagcgcca gtttgaagca ggaatttata
    32101 agggaagctg gagtcaggca tcacgctaac ggcacataaa catccgtttc cagtcgctgc
    32161 tctgtcaccc cttcggcaag attggtgtag tgaaacagaa tcggtgcttt acgcattttc
    32221 tcgccgcttg caggcagcca gtggcgaata atgccccata tcgtgtgtga aatatcgtcc
    32281 agctcgccaa cgtggcgggc cacggcataa cgtccaccgg taagttcacc attgctcaca
    32341 ccataacgat tatcgggaat cggttcgcta acgctgccgc agatatcaaa gcgaaacgct
    32401 tccggtgcgg tggtggcagg gtcgtcccag gcgacgccaa aagtctggct ttggttaaca
    32461 ggagaaagcc ccgtctcttt acgccacatg ataaatttcg ccacactgta gttgagcaac
    32521 tcagggctac agcggtgcgg caacatcgcc acagggattg atgggaaatc gataatgttt
    32581 acatccagtg tcaggtttgt cattttgcct ccctgcgaaa ttattcccgg gaacgcgtcc
    32641 cgggagcagt aacaattacg gatttacttt gcggatctgt ttgacgtcaa tttcaacaga
    32701 attccagtct ttatcgactt caccctgaat ctcaaccgta tctttcggcg tcaccgtcac
    32761 gccgttccag cgtttgtgat cgatatcaac attgatagta ccgctggcat ctttgaacac
    32821 gtagagatcg tcagagatgc gttcaacgat attgccgcgc agggttaccc aggtgtcgtc
    32881 acgcagggat tttgcgcttt ctacagtcgt tacgctgccg ttcggcccct ggaatcctcc
    32941 ggcctgactt tgcgttgccg atgggccaga aaaaccgccc tgctctgctg ccatcaccgg
    33001 tgcgctgcac agggccatta ctgcgattac tgctgcgaat tttttcatgt ttattactcc
    33061 ctttaatgtc tgtttccgag catttaacaa gatagtcctt aacaacttct taagggaaaa
    33121 aaataaaatt tagtgctgta cagagcgcgt tacaacacgg tttactggca gcaaatacgg
    33181 ttatcgcagg gatgaaaaaa tgcgaatttt actgatagaa gatgacatgc tgattggcga
    33241 cggcatcaaa acgggcctta gtaaaatggg ttttagcgtc gactggttta cacaaggtcg
    33301 tcagggaaaa gaggcgcttt atagcgcacc ttatgatgcg gtgatcctgg atttaacctt
    33361 accaggcatg gatggtcgcg atattttgcg cgaatggcga gaaaaaggtc agcgtgagcc
    33421 ggtactgatc ctgaccgcgc gcgatgcgct ggcggaacgt gtagaagggc tgcgtctggg
    33481 agctgacgat tatctgtgta aaccttttgc gttgatagaa gtcgccgcca ggctggaagc
    33541 tctgatgcgc cgaaccaacg gccaggccag caacgagctg cgccacggta acgtcatgct
    33601 cgaccccgac aaacgtatcg ccacgctggc tggcgaaccc ttaacactga aaccaaaaga
    33661 atttgccctg ctggaattac tgatgcgtaa cgctggtcgg gtactgccgc gcaaactgat
    33721 tgaagagaaa ctgtatacct gggacgaaga ggtcaccagt aatgccgttg aagtgcatgt
    33781 gcatcatctg cgacgcaaac tcggtagtga ttttattcgt accgtgcatg gtattggcta
    33841 cacattaggt gagaaatgaa atttacccaa cgtcttagtc tgcgcgtcag gctgacgcta
    33901 atctttttaa ttctggcctc ggtgacctgg ctgctttcca gctttgtcgc ctggaaacaa
    33961 acaacggata acgtcgatga attgttcgac acccaactga tgctgtttgc caagcggtta
    34021 agtacgctcg atctcaacga aatcaacgcg gcggatcgca tggcacagac gccaaataga
    34081 ttaaaacacg gtcatgttga tgacgatgcg ctgacctttg ccatctttac ccacgacggc
    34141 agaatggtcc ttaatgatgg cgataacgga gaagatattc cctatagcta tcaacgggaa
    34201 ggttttgctg acgggcaact ggtcggtgaa gacgatcctt ggcgttttgt ctggatgacc
    34261 tcacctgatg gcaaatatcg catcgttgtt ggccaggaat gggaataccg tgaagacatg
    34321 gcgctggcga ttgttgccgg gcaattgatc ccgtggctgg tcgcactgcc gattatgtta
    34381 atcatcatga tggtactact gggtcgtgaa ctcgcgccgc tgaacaaact ggcgctggca
    34441 ctacgtatgc gtgaccctga ctcggaaaaa ccactaaacg cgactggcgt acccagcgaa
    34501 gtgcggccac tggttgagtc gctaaatcaa ctgttcgccc gcacacatgc gatgatggtt
    34561 cgtgaacgac gctttacctc cgacgcagct cacgaacttc gtagcccgtt aacggcgctg
    34621 aaagtgcaaa ccgaagttgc gcagctctct gacgatgatc cgcaggcgcg gaaaaaagca
    34681 ctgcttcaat tacattccgg gatcgatcgc gctactcgtc tggttgatca actgctcacg
    34741 ctatcgcggc tggactcact ggataacctt caggacgtcg cggagatccc gcttgaagat
    34801 ctcctgcaat cgtcggtgat ggatatttac cacacggcgc agcaggcgaa aattgacgtg
    34861 cgactgacac tcaatgtcca gggcatcaaa cgcaccggac aaccgctatt gctaagtttg
    34921 ttggtgcgaa atttgctgga taacgccgtg cgttacagtc cacagggcag cgtggtggac
    34981 gtcacgctga atgccgataa tttcatcgtg agggataacg gccccggtgt gacaccagag
    35041 gcactggcgc gaattggcga acgcttctat cgcccacccg gacaaaccgc caccggcagt
    35101 ggacttgggc tatcgattgt ccagcgaatc gccaagctgc atggcatgaa tgttgagttt
    35161 gggaatgcgg aacaaggtgg atttgaggcg aaggtaagct ggtaagggta agacttttgc
    35221 taaattcgca aaagactttg cacattttgc taatttcacc gtaccgctct gtgacgtact
    35281 atagtcgaca aacgtctcac cttgaggtta aaaaatgagc aacatcctga ttatcaacgg
    35341 cgcgaaaaaa ttcgcccact ccaatggtca actgaacgac accctgaccg aagtcgcgga
    35401 tggcacactg cgcgacctcg ggcatgatgt ccgcatcgtt cgcgccgaca gcgactacga
    35461 tgtcaaagcg gaagtacaaa actttctctg ggctgatgtg gtgatctggc agatgccagg
    35521 ctggtggatg ggcgcgccgt ggacagtgaa aaaatacatt gatgatgtat tcaccgaagg
    35581 tcacgggact ctgtatgcca gcgatggtcg tacccgcaaa gatccgtcga aaaaatacgg
    35641 ctccggcggc ctggtacagg gcaaaaaata tatgctttct ctgacctgga acgcaccaat
    35701 ggaagccttc accgaaaaag atcagttctt ccatggcgtt ggcgttgacg gcgtgtatct
    35761 gccgttccat aaagcaaacc aattcctcgg tatggaaccg ctgccgacat ttatcgctaa
    35821 tgacgtgata aaaatgcctg atgttccccg ctatactgaa gaatatcgca agcatcttgt
    35881 ggaaattttt ggttaactag agctcaggct ttagaaggag ttaaccatgc ttaccgtaat
    35941 cgcagaaatc cgtactcgtc ctggtcaaca tcaccgtcag gcggtattgg atcagtttgc
    36001 taaaatcgtt ccaaccgtac tgaaagaaga aggttgccac ggctatgcgc caatggtgga
    36061 ttgcgctgct ggcgtgagtt tccagtctat ggcaccggat tctatcgtga tgatagagca
    36121 gtgggaaagc atcgcgcatc ttgaagcgca tctgcaaacc ccgcacatga aggcgtatag
    36181 cgaagccgta aaaggtgacg tgctggagat gaatattcgt attctgcagc cagggattta
    36241 acattcctga aaagaggccg gtgctgtttg ccggccattt atattcactc ttccgccgtc
    36301 tgtaaccgtg ctttaatctg ttcgtaaaat tcattattaa tcagataaaa tcgcatgatg
    36361 agtccggtaa tcaggttaag tatcgcggga aagatcgtca ttaacaggca gatcccccac
    36421 agcgcctgtg gtgtttgttc tacaccaggt tgataatcca aataggctaa agcgattccg
    36481 ataaagcccc ctgccagccc cataccgaac ttttggaaaa agagaatccc gccaaatgcc
    36541 aggccagaaa cacgttgccc cgttttcatt tcaccgtaat ccacggactc agcaatggat
    36601 gcccaataaa tgggaagctg aatatccgtc aggaaggtca ccacaatata aaatgtaaaa
    36661 gctaacacta tagaatttgg ttgcacgaaa tacatcgcac cacccaccac gaacgccagt
    36721 aattgggtat aacgaaatat ttttattttg tcgtagagtc gagtaagcca ggtacaagcg
    36781 atcatagcca gaacagaggc gatcacgcct gaagttaaaa acggtgaaat taaagcatcc
    36841 ccaccattca ggtaatattt tgcataatat gcagcgacag agttacgaat aatcccgcca
    36901 aacataatga tcgcaattac agcgcccaaa atgagccatt gatcattttt gatcagtaac
    36961 cgaaactgcg cggccatacc gagactgggt tttggatggt agatgcgctc tctcacgcta
    37021 aaaaaacaat acagacataa acacgttgcc agaattccca ttgcccccat tgctatttga
    37081 taacccagtt ttacgttatt cttcccaaga tacattgcag ataaaggaac aataattgtt
    37141 acggcgaaca tggcaatttt tgtcataaca aaacgataac cattggcgct caatcgctct
    37201 tgtggatcat ccgtgataac gctgattaat gaaacataag gaatagcgat taacgtatag
    37261 gtcaaggtaa gtaacacata ggtagcccag gcccataaaa gcttaactga ataatcggta
    37321 tcaggtgtgg tgaacatcaa ccaaatgctt atcccaaacg gtattgacat aaaaagcaac
    37381 cacggccgat attttcccca acgtgtattc gttctgtctg tcattgttcc cacaactgga
    37441 tcaatcacag catcaaggat acgtacagaa aataacagaa tacctaagtc gacgggtttc
    37501 agaccaaata catccgtgta aaaataagtg atgatcatgg ctaaagacat cattactatc
    37561 gcaatagcca tatcaccggc accaaaacct atcttttcat ttattgtcag tttcatacac
    37621 agcactcatt gatcacgtga aattatatta aaaacaaatg tatcggaact cattcagcac
    37681 ataactttac gagtgagaat aaatgcattg gtagat
//
LOCUS       DBANUU010000033        37647 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000033 DBANUU010000000
VERSION     DBANUU010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.033"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            219..1592
                     /gene="aroP"
                     /locus_tag="ACIE68_001619"
     CDS             219..1592
                     /gene="aroP"
                     /locus_tag="ACIE68_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFX5907951.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1635..2489)
                     /gene="ampE"
                     /locus_tag="ACIE68_001620"
     CDS             complement(1635..2489)
                     /gene="ampE"
                     /locus_tag="ACIE68_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFX5907952.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2486..3037)
                     /gene="ampD"
                     /locus_tag="ACIE68_001621"
     CDS             complement(2486..3037)
                     /gene="ampD"
                     /locus_tag="ACIE68_001621"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFX5907953.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            3125..4018
                     /gene="nadC"
                     /locus_tag="ACIE68_001622"
     CDS             3125..4018
                     /gene="nadC"
                     /locus_tag="ACIE68_001622"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFX5907954.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4221..4661
                     /gene="ppdD"
                     /locus_tag="ACIE68_001623"
     CDS             4221..4661
                     /gene="ppdD"
                     /locus_tag="ACIE68_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFX5907955.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTHYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4671..6056
                     /gene="gspE"
                     /locus_tag="ACIE68_001624"
     CDS             4671..6056
                     /gene="gspE"
                     /locus_tag="ACIE68_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFX5907956.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLARTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMCTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6046..7248
                     /gene="hofC"
                     /locus_tag="ACIE68_001625"
     CDS             6046..7248
                     /gene="hofC"
                     /locus_tag="ACIE68_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFX5907957.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAILHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRENTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7283..8326)
                     /gene="guaC"
                     /locus_tag="ACIE68_001626"
     CDS             complement(7283..8326)
                     /gene="guaC"
                     /locus_tag="ACIE68_001626"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFX5907958.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGYEESGGLIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8482..8526)
                     /locus_tag="ACIE68_001627"
     CDS             complement(8482..8526)
                     /locus_tag="ACIE68_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5907959.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8551..9171
                     /gene="coaE"
                     /locus_tag="ACIE68_001628"
     CDS             8551..9171
                     /gene="coaE"
                     /locus_tag="ACIE68_001628"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFX5907960.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9171..9914
                     /gene="zapD"
                     /locus_tag="ACIE68_001629"
     CDS             9171..9914
                     /gene="zapD"
                     /locus_tag="ACIE68_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFX5907961.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9924..10121
                     /gene="yacG"
                     /locus_tag="ACIE68_001630"
     CDS             9924..10121
                     /gene="yacG"
                     /locus_tag="ACIE68_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFX5907962.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10337..10726)
                     /gene="mutT"
                     /locus_tag="ACIE68_001631"
     CDS             complement(10337..10726)
                     /gene="mutT"
                     /locus_tag="ACIE68_001631"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFX5907963.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVERWEGKPWGKEGQPG
                     EWMALVDLNADDFPPANEPVIAKLKRL"
     gene            complement(10786..13491)
                     /gene="secA"
                     /locus_tag="ACIE68_001632"
     CDS             complement(10786..13491)
                     /gene="secA"
                     /locus_tag="ACIE68_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFX5907964.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13553..14065)
                     /gene="secM"
                     /locus_tag="ACIE68_001633"
     CDS             complement(13553..14065)
                     /gene="secM"
                     /locus_tag="ACIE68_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFX5907965.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(14296..15213)
                     /gene="lpxC"
                     /locus_tag="ACIE68_001634"
     CDS             complement(14296..15213)
                     /gene="lpxC"
                     /locus_tag="ACIE68_001634"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFX5907966.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15314..16465)
                     /gene="ftsZ"
                     /locus_tag="ACIE68_001635"
     CDS             complement(15314..16465)
                     /gene="ftsZ"
                     /locus_tag="ACIE68_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFX5907967.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16526..17788)
                     /gene="ftsA"
                     /locus_tag="ACIE68_001636"
     CDS             complement(16526..17788)
                     /gene="ftsA"
                     /locus_tag="ACIE68_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFX5907968.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17785..18615)
                     /gene="ftsQ"
                     /locus_tag="ACIE68_001637"
     CDS             complement(17785..18615)
                     /gene="ftsQ"
                     /locus_tag="ACIE68_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFX5907969.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18617..19537)
                     /gene="ddlB"
                     /locus_tag="ACIE68_001638"
     CDS             complement(18617..19537)
                     /gene="ddlB"
                     /locus_tag="ACIE68_001638"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFX5907970.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19530..21005)
                     /gene="murC"
                     /locus_tag="ACIE68_001639"
     CDS             complement(19530..21005)
                     /gene="murC"
                     /locus_tag="ACIE68_001639"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFX5907971.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21059..22126)
                     /gene="murG"
                     /locus_tag="ACIE68_001640"
     CDS             complement(21059..22126)
                     /gene="murG"
                     /locus_tag="ACIE68_001640"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFX5907972.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22123..23367)
                     /gene="ftsW"
                     /locus_tag="ACIE68_001641"
     CDS             complement(22123..23367)
                     /gene="ftsW"
                     /locus_tag="ACIE68_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFX5907973.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23367..24683)
                     /gene="murD"
                     /locus_tag="ACIE68_001642"
     CDS             complement(23367..24683)
                     /gene="murD"
                     /locus_tag="ACIE68_001642"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFX5907974.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKICVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24686..25768)
                     /gene="mraY"
                     /locus_tag="ACIE68_001643"
     CDS             complement(24686..25768)
                     /gene="mraY"
                     /locus_tag="ACIE68_001643"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFX5907975.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGIIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25762..27120)
                     /gene="murF"
                     /locus_tag="ACIE68_001644"
     CDS             complement(25762..27120)
                     /gene="murF"
                     /locus_tag="ACIE68_001644"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFX5907976.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILNQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIKLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDCVLSVGK
                     QSHAISTASGVGEHFTDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27117..28604)
                     /gene="murE"
                     /locus_tag="ACIE68_001645"
     CDS             complement(27117..28604)
                     /gene="murE"
                     /locus_tag="ACIE68_001645"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFX5907977.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLAELLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28591..30357)
                     /gene="ftsI"
                     /locus_tag="ACIE68_001646"
     CDS             complement(28591..30357)
                     /gene="ftsI"
                     /locus_tag="ACIE68_001646"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFX5907978.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMAEYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30373..30738)
                     /gene="ftsL"
                     /locus_tag="ACIE68_001647"
     CDS             complement(30373..30738)
                     /gene="ftsL"
                     /locus_tag="ACIE68_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFX5907979.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30735..31676)
                     /gene="rsmH"
                     /locus_tag="ACIE68_001648"
     CDS             complement(30735..31676)
                     /gene="rsmH"
                     /locus_tag="ACIE68_001648"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFX5907980.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGDYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31678..32136)
                     /gene="mraZ"
                     /locus_tag="ACIE68_001649"
     CDS             complement(31678..32136)
                     /gene="mraZ"
                     /locus_tag="ACIE68_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFX5907981.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32738..33742)
                     /gene="cra"
                     /locus_tag="ACIE68_001650"
     CDS             complement(32738..33742)
                     /gene="cra"
                     /locus_tag="ACIE68_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFX5907982.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33824..33910)
                     /locus_tag="ACIE68_001651"
     CDS             complement(33824..33910)
                     /locus_tag="ACIE68_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFX5907983.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33922..34413)
                     /gene="ilvN"
                     /locus_tag="ACIE68_001652"
     CDS             complement(33922..34413)
                     /gene="ilvN"
                     /locus_tag="ACIE68_001652"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFX5907984.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGEK
                     IMR"
     gene            complement(34416..36140)
                     /gene="ilvI"
                     /locus_tag="ACIE68_001653"
     CDS             complement(34416..36140)
                     /gene="ilvI"
                     /locus_tag="ACIE68_001653"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFX5907985.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESVHQPLDEIRDWWQQIEQWRARQCLKYDSHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36258..36302
                     /locus_tag="ACIE68_001654"
     CDS             36258..36302
                     /locus_tag="ACIE68_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5907986.1"
                     /translation="MKRNDKILHYRGLN"
     gene            complement(36457..37401)
                     /gene="leuO"
                     /locus_tag="ACIE68_001655"
     CDS             complement(36457..37401)
                     /gene="leuO"
                     /locus_tag="ACIE68_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFX5907987.1"
                     /translation="MPEVQTDQPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQTAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEDFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9083 a        10468 c         9181 g         8915 t
ORIGIN      
        1 ctgagcttta caaacggttt ctttttaagc aactcatctt caaccatgca taaagcgggt
       61 gcattcgctg ccgcatacca ttattcttga tctgacggaa gtctttttgt aacaattcaa
      121 acttctttga tgtaaacaaa ttaatacaac aaacggaatt gcaaacttac acacgcatca
      181 ctgcgtagat caaaaaaaca accaccgcac gaggtttcat gatggaaggt caacagcacg
      241 gcgagcagct aaagcgcggc cttaaaaacc gccatattca gcttatcgcg ctgggtggcg
      301 cgatagggac cgggttattc ctgggtagcg cctccgtaat acagtccgca gggccaggga
      361 ttatcctggg ttacgccatt gctggtttta tcgcctttct gatcatgcgt cagctgggtg
      421 aaatggtggt cgaagaacct gtcgcaggct cctttagcca ctttgcttat aaatactggg
      481 gcagttttgc cggtttcgcc tctggctgga actactgggt actgtacgtt ttagttgcca
      541 tggctgagct gactgccgtg ggtaaataca ttcagttctg gtatccggaa atccccacct
      601 gggtttctgc cgccgtattc tttgtggtga ttaacgccat caacctgacc aacgttaaag
      661 tgtttggcga gatggagttc tggtttgcca ttatcaaagt tatcgcggtg gtagcgatga
      721 tcatcttcgg cggctggctg ctattcagtg gcaacggcgg cccgcaggcg accgttagca
      781 acctgtggga tcagggtggt ttcctgccgc acggcttcac cgggctggtg atgatgatgg
      841 cgattatcat gttctcgttc ggtggtctgg aactggtggg gatcaccgca gcagaagctg
      901 ataacccgga gcaaagtata ccgaaagcaa ctaaccaggt tatctaccgc atcctgattt
      961 tctatattgg ttcgttagcc gttctgctct cactgatgcc gtggacccgc gttaccgccg
     1021 ataccagtcc gtttgtgctg atcttccacg agttaggcga tacctttgtg gcgaatgcgc
     1081 tgaacatcgt ggtactgact gcggcgctct ccgtgtacaa cagctgcgta tattgcaaca
     1141 gccgtatgct gtttggtctg gcacaacagg gtaatgcgcc aaaagcgctg gcgtctgtcg
     1201 ataaacgtgg tgtaccagta aataccattc tggtgtctgc actggtaacg gcgttgtgcg
     1261 tactgattaa ctaccttgcc ccagagtccg ctttcggact gttaatggcg ctggtggtat
     1321 ctgcactggt aatcaactgg gcgatgatta gcctggcgca tatgaaattc cgtcgcgcca
     1381 agcaggaaca aggcgtggta actcgcttcc ctgctctgct ttatccgctg ggtaactgga
     1441 tctgcctgct gtttatggcg gcggtactgg tgattatgct gatgacccca ggaatggcga
     1501 tttcggtata cctgatcccg gtatggctga tcgtgttagg tatcggctat ctgtttaaag
     1561 agaaaaccgc caaagccgta aaagcgcatt aatctctcta cgccctcacc cgtacagggt
     1621 gagggcaata atctttacac caacgccccg taaatcgtca gtagtgcaat caccaccacg
     1681 accacgaacg aggttttctt cgccattgaa accgctgcct tcggcgtctc caccttatcg
     1741 acatgcggtt cacgcgccag agagaactgc gccagacgcg ttaacacctg atactgcgaa
     1801 gtatggaaat cacccagcga agcaaaccag gccggtaacg ctttctcacc atgaccgatc
     1861 aaggcatata ccacacccgc aagacgaacc ggcacccaat ccagtacatg aagcacggca
     1921 tcaatgccgg actgtaaacg atgatgcggc gtctgatatc gcgccagcca gtattgccat
     1981 gcacgcaaaa acgcataccc catcagcgta acgggtcccc aggttccccc cacaatcagc
     2041 cagaacagcg gtgcaagata aaaacgaaag ttaatccaca gcaatgcatt ttgcagctca
     2101 cgcaaatact cacgttcgtc gcagcctgcc gggacgccgt gaatcatggt gagttcgcca
     2161 gccatcgtgg cacgggcatg gctatcatta cgtgaagcag ctgtcagata agcatgataa
     2221 tgaagacgaa ctttacctgc gccaatacac agcaaaccaa tcagcagcca caccagtagc
     2281 gtgggaacgt tgaacaatac tccctgcaat gcgcgtaaca gtaaaaaagt cacgcccatc
     2341 gcaataatgg tcatgcctaa cgtgcgcccg agagaaaaat gtttcacccg ccgaaagaac
     2401 gcttcaagac gatgatcaag ctgccagtgc tcgcccaact taaacaggcg ctcgaaaatt
     2461 aacaccagta aggttgtaaa tagcgtcatg ttgtctcctt gctgaccagc acacgaaacc
     2521 gtgcccaatc aaatgcagga ccgggatcgg ttttccgatc cggcgcaata tcacaatggc
     2581 ccgtcatgtt tttagcgata tccggatagc aatcaatcag tgcccgcgta accgccgcaa
     2641 gctgttgata ctgcgcatcg gtatacgcca gcgtatcggt gccttcaagc tcaatcccaa
     2701 tagaaaaatc attgcagcgt tcgcgcccct gatactgaga gactcccgca tgccatgcac
     2761 gtttatcgaa aggaacatac tggactattt caccatcacg gcgaatcaaa cagtgagcgg
     2821 agacgcgcaa atgggcgatc tcagcaaaga aaggatgtgc ctgcggatca atagttccag
     2881 tgaataatgc gtcgatccac ggaccgccaa actcgcctgg cggcaggcta atattgtgca
     2941 ccaccagcag ggtgggtgtt tcgtcatccg ggcggcaatc gtaatgtggt gagggaacgc
     3001 ggcgcgcgcc aaccagccac ccctgttcta acaacatgca ggatctcctt atatgtggtg
     3061 ctaatacccg gttcagagta gcatgtttct accttatgat tcgttagcta tctggagttt
     3121 taacatgccg cctcgccgct ataaccctga cacccgacgt gacgagctgc tggaacgcat
     3181 taatctcgat atccccggcg cggtggccca ggcgctgcgg gaagatttag gcggaacagt
     3241 cgatgccaac aatgatatta cggcaaaact tttaccggaa aattctcgct ctcatgccac
     3301 ggtgatcacc cgcgagaatg gcgtcttttg cggcaaacgc tgggttgaag aggtgtttat
     3361 tcaactggca ggcgacgatg tcaccataat ctggcatgtg gatgacggcg atgtcatcaa
     3421 tgccaatcaa tccttgttcg aacttgaagg cccatcccgc gtgctgttaa cgggcgaacg
     3481 cactgcgctt aattttgtgc aaaccctttc aggagttgcc agtaaggtac gccactatgt
     3541 cgaattgctg gaaggcacca acacgcagtt gttggatacg cgcaaaacct tacccggcct
     3601 gcgttcagct ctgaaatacg cggtactttg cggcggcgga gcgaatcacc gtctggggct
     3661 ttctgatgcc ttcctgatca aagaaaacca tattattgcc tccggctcag tgcgccaggc
     3721 ggtcgaaaaa gcgtcctggc tgcacccgga tgcgccagta gaagtcgaag tagagaatct
     3781 ggaagaactt gatgaagccc tgaaagcagg agccgatatc atcatgctgg ataacttcga
     3841 aacagaacag atgcgcgaag ccgtcaaacg caccaacggc aaggcgctac tggaagtgtc
     3901 tggcaacgtc actgacaaaa cactgcgtga atttgccgaa acgggcgtgg actttatctc
     3961 cgtcggtgcg ctaactaaac acgtacaagc actcgacctt tcaatgcgtt ttcgctaata
     4021 gttgacagat ttatccgctc catcgcgggc ggataatctc cccttcccca actttcttcg
     4081 taacgcctcg caaatttctt gtctttcgct gcaacgaaat agaaaacctc ggaatctgct
     4141 ttcccgtttc gttttttgcc cctcgcctgt gctgatggcg tttgccaaag tagcgccaac
     4201 caaatcaagg agcgaaacag atggacaagc aacgcggttt tacacttatc gaactgatgg
     4261 tggttattgg catcattgcc attttaagcg ccattggtat tcccgcttat caaaactacc
     4321 tgcgcaaagc cgcactcacc gacatgctac aaacctttgt gccttaccgt accgccgtag
     4381 agttgtgcgc gctggaacat ggtggattag atacctgcga cggtggcagc aatggcattc
     4441 cctcgcctac caccacccac tatgtttcag ccatgagtgt ggcaaaaggc gtggtgtcgc
     4501 tgaccgggca agaaagtctc aatggactaa gcgtcgtcat gacaccgggt tgggataacg
     4561 caaacggcgt caccggctgg acgcgcaact gcaatattca aagtgacagc gcattgcagc
     4621 aagcctgcga agatgtcttc cgctttgatg acgccaacta aggagcggca atgaatattc
     4681 cacagctcac ggccctgtgt ctgcgttatc agggagtctt gctggatgcc agcgaagaag
     4741 tggttcatgt tgcggtggtc gatgccccct cacatgagtt gctggacgca ttgcatttcg
     4801 ctaccaccaa acgtattgag atcacctgct ggacgcgcca acaaatggaa ggtcacgcca
     4861 gtcgcacaca acagacattg cccgtagctg ttcaggagaa gcatcagccc aaagcagagt
     4921 tgctagctcg aacgttacaa tctgcgctgg aacaacgcgc gtctgatatt catatcgaac
     4981 cagcggacaa tgcctaccgc atccgcttgc gtatcgacgg cgtattgcat cctttaccgg
     5041 atgtttcacc ggatgccgga gtcgcattaa ccgccagatt aaaagtgctg ggaaacctgg
     5101 atattgcgga acatcgcctg ccgcaggacg ggcaattcac tgtcgaactg gcaggaaacg
     5161 ccgtctcatt tcgtattgcg accttaccat gtcggggtgg tgaaaaggtg gtattaaggt
     5221 tgttacagca ggtgggtcag gcactggatg tcaacacgct tggaatgcag ccgttacaac
     5281 tggcggactt tgctcatgcc ttgcaacaac cacagggact ggtgctggta actggcccta
     5341 ccggcagcgg caaaacggtc acgctttata gtgccctgca aacgctgaat accgctgaca
     5401 ttaatatttg tagcgtcgaa gatccggttg agatccccat agccggacta aaccagacgc
     5461 aaatccatcc gcgtgccggg ctcacctttc agggcgtttt gcgtgcgtta ttgcgccagg
     5521 atcctgacgt catcatgatc ggagagatcc gcgatggcga aacagcagag atcgctatta
     5581 aagcggcgca aactggtcac ctggtgttgt ctaccctaca cactaattcc acctgcgaaa
     5641 cgctggtacg tttacagcaa atgggagtcg cccgctggat gctctcatca gcgcttacgc
     5701 tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca ttgtcgccag cagcaagggg
     5761 agcccatcca tattccagtc aatgtatggc cgtcgccgct gccccactgg caggcacccg
     5821 gttgtgtaca ttgctaccac ggtttttatg gtcgtacggc cttatttgaa gttctgccca
     5881 taacgccggt cattcgtcag cttatttccg ctaataccga cgttgaatcg ctggaaacgc
     5941 acgcacgaca ggcgggtatg tgtacgcttt ttgaaaacgg ctgcctggcc gtggagcaag
     6001 gcttaaccac ctttgaagag ttaatccgcg tactggggat gccgcatggc gagtaagcaa
     6061 ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc aagatgggat gctatgggca
     6121 gagagccgtg ctttgctgct catggcacta cagcaacaga tggttacccc acttagcctg
     6181 aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata aaagcgcgga agtcattcat
     6241 caactggcga cgctactcaa agccgggtta acgctttctg aagggctggc actgctggcg
     6301 gaacagcatc ccagtaagca atggcaagcg ttgctgcaat cgctggcgca cgatctcgaa
     6361 cagggcattg ctttttccaa tgccttatta ccctggtcag aggtatttcc gccactctat
     6421 caggcgatga tccgcacggg tgaactgacc ggtaagctgg atgaatgctg ctttgaactg
     6481 gcgcgtcagc aaaaagccca gcgtcagttg accgacaaag tgaaatcagc gttacgttat
     6541 cccatcatca ttttagcgat ggcaatcatg gtggttgtgg caattctgca ttttgttctg
     6601 ccggagtttg ccgctatcta taagaccttc aacaccccac taccggcact aacgcagggg
     6661 atcatgacgc tggcagactt tagtggcgaa tggggctggc tgctggtgtt gttcggcttt
     6721 ctgctggcga tagccaataa gttgctgatg cgccgcccta cctggcttat cgcgcggcag
     6781 aaattgttgt tacgaatccc gattatgggt tcactgatga ggggacaaaa actcacgcag
     6841 atctttacga ttctggcgct gacacaaagt gcaggcatta cttttttaca gggcgtagag
     6901 agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac ttctgacaca aatccagcac
     6961 gatatcagta acggtcatcc catctggctg gcgctaaaaa atgccggtga gtttagcccg
     7021 ctctgtttgc aattagtgag aacaggagag gcatccggct ctctggatct catgttagac
     7081 aacctcgccc atcatcatcg ggaaaacaca atggcgctgg cggataacct cgcagcctta
     7141 ctggaaccgg cgttgctgat cataacggga ggaattattg gtacgctggt ggtggcaatg
     7201 tatctgccaa ttttccattt aggcgatgcg atgagtggga tgggataacg ctggcgtgtt
     7261 gctccacgcc agcgttggga gattacaggt tgttgaagat gcggttttct tgttcctgca
     7321 cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg tgaagcccca acgtatgtac
     7381 aagctgaacg caggccgccc aaaatatctc gcgcggtatt ttcaaccggg cctcgcagcg
     7441 gcagcttaac ggttttacct tctgctgcgc gatattccgc aacgccgcca acgtgacgtt
     7501 tcatcgcaga ctcggagctc atgccgtaga acagcataaa tttctcgccg ttctcctcaa
     7561 cgatgagacc gccgctctct tcgtagcccg ccagcatgcc gccaagcatg acgaaatcgg
     7621 caccgccgcc aaaggctttc gccacatcgc ccggcgtggt gcagccacca tcgctgacga
     7681 tcattccgcc cagaccgtgc gcagcatcgg cacattcgat taccgcagaa agttgcggat
     7741 aaccgacgcc tgttttgacg cgagttgtac aaacagaacc tgggccaatg ccaactttaa
     7801 cgatatcggc acctgagagg ataagctcct cacacatttc accagtcact acgttaccag
     7861 cacaaatggt tttggtcggc cacgcttcac gcgctttcgc aacgaactgc acgaagtgtt
     7921 cggaataacc attcgccacg tcaatacaaa cgaagtttaa tgccgggttc aggtcgagaa
     7981 tctgtttagt tttttcgaaa tccgcatcag acgtaccggt agaaaccatc acatgtttca
     8041 gcacatcagc ggaagaattg ttgataaacg cttgccactc ttcgacagaa tagtgtttat
     8101 gcacagcagt caaaatatca aaagaagcca gcgcagaggc catagaaaat gtgcctacgg
     8161 tgtccatatt tgcggcgata atcggcacgc cggaccagct ctgacctgaa tgtttgaagg
     8221 tgaattgacg ttccagttca acatcggaac ggcttttaag agtggagcgt ttagggcgga
     8281 tgagaacgtc tttaaaacct aacttcagat cttcttcaat acgcatgtgc ggattcctgg
     8341 ggttaatggc tataagggta agagcgcaac tccagtgacg ctatcatacg cactaatcaa
     8401 tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca atcccataaa tttacctggt
     8461 aaatagtgag cgaacgtttc atcaatttcg ctttcgtgca ctcattttta acgttttgat
     8521 aatcatgatg aattcccggg aatataaatt atgaggtata tagttgcctt aacgggaggc
     8581 attggcagtg gcaagagtac cgttgccaat gcgtttgctg atctcggaat taacgtcatt
     8641 gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg cacctgcgct acatgccatt
     8701 gctgatcact ttggcgctaa catgattgct gctgatggaa cattgcagcg ccgggccttg
     8761 cgcgagcgga tcttcgccaa cccggaagag aaaaactggc ttaacgccct gctgcatccg
     8821 ctgattcagc aagagacgca acaccagatc cagcaagcta cttcccccta tgtactgtgg
     8881 gttgtgccat tgctggtaga aaactcactg tataaaaaag cgaatcgagt gctggtggtg
     8941 gatgtcagcc cagaaacgca acttaagcgc accatgcagc gcgatgatgt aactcgcgag
     9001 catgtcgaac aaatccttgc tgctcaggca acgcgcgaag cccgccttgc cgtggcagat
     9061 gacgtcattg ataataacgg cgcaccggat gctatcgcat cggatgttgc ccgcctgcac
     9121 gcacactatt tgcagcttgc gtcgcagttt gtctcacagg aaaaaccgta atgcagaccc
     9181 aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac atggctgcgc attgagtttt
     9241 tgattcagca actcaccgtt aatttaccca tcgttgacca cgctggcgcg ctgcatttct
     9301 tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg cgaagtccgc actgagctgt
     9361 tgaaagaact tgaccggcag caacgtaaac tccagacctg gattggcgtg cctggcgtgg
     9421 accagagccg tattgaagca ttaattcagc agttaaaagc ggcggggagc gtattaattt
     9481 ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt gattgctctg gtgcgtcagc
     9541 gactgagcat cccaggcggc tgttgcagct ttgatttacc tacattgcac atttggctgc
     9601 atctacccca ggcgcagcgc gacagccagg tagaaacctg gattgccagc ctgaacccgc
     9661 tcacccaggc acttaccatg gtgctggatt taattcgcca gtcggccccc ttccgtaaac
     9721 aaaccagcct gaatggtttt tatcaggata acggtggcga tgccgacttg ctgcgcctga
     9781 atctgtcgct cgattcacag ctttatccgc aaatttccgg acataagagc cgttttgcca
     9841 ttcgttttat gccgctggac actgaaaacg gacaggtacc ggaacgtctg gatttcgaac
     9901 tggcctgttg ctaaggagta aaaatgtcag aaactattac ggtgaattgc ccaacctgcg
     9961 ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc attttgctcc aaacgttgtc
    10021 agctgatcga cctcggagaa tgggctgctg aagaaaaacg aatccccagc agtggcgatc
    10081 tttccgaaag cgatgactgg agcgaagagc caaagcagtg acatttgtct gatgccgcac
    10141 gtaggcctga taagacgcgg acagcgtcgc atcaggcatc ttgtgcaaat gtcggatgcg
    10201 gcgtgaacgc cttatccgac ctacaaaaac aaatttatca atgagttaga aatccctgta
    10261 ggcctgataa gacgcgccag cgtcgcatca ggcatcttgt gcgaatgtcg gatgcggcga
    10321 aaacgcctta tctgacctac agacgtttaa gcttcgcaat taccggttca ttggctggcg
    10381 gaaaatcatc ggcattaaga tcgaccagcg ccatccactc accgggttgc ccttctttac
    10441 cccacggctt cccttcccag cgttcgacca gccagaacca cagtgttata tgcctgtccg
    10501 ggaattcata ttccagtttt tcaaatagcg aaaaatgttg tggggtaatc ccgacttctt
    10561 cctgaagttc acgcaccacc gcctgttccg gcgtttcacc catttcaatt ttaccgccgg
    10621 gaaactccag tttattcgcc atgtgcgcat ctgctgcgcg acgcgttata aagatttcat
    10681 tgttctcgtt gcgaataata cctaccgcaa tttgcagctt tttcattgtc ttaaacctat
    10741 aaaaaaggcg cagaatcctg cgccttttac ttcaactgtt agcttttatt gcaggcgacc
    10801 atggcactgc ttgtattttt taccagaacc gcacgggcaa ggatcgttac gtcctacttt
    10861 gcgttcaccg gtttgcgccg ccagtgcagc tgcggctgca gagtcgtcat cctgatggct
    10921 aagctgctgc atttgcgcta aacgctcggc ttccatacga cgctgttgtt ccagctcctc
    10981 aacttcttca ggcatacgta cctgaacttt gctcagcgta ctgataactt catatttcaa
    11041 cgactccagc atcgctgcaa acatggagaa cgattcacgt ttgtattcct gcttcggatc
    11101 tttctgtgca tagcctcgca ggtggatacc ctgacgcaga tagtccatcg ctgccaggtg
    11161 ctctttccac agggagtcaa gcgtttgcag catgacgcct ttctcgaagt gacgcatcat
    11221 ctctgcacca accacttctt ctttacgctg atacacttcg atggactgcg ccagaatgcg
    11281 ctcacgcagc gtctcttcat gcagttcggg ttctttatcc agccactcag caattggtaa
    11341 atcgaggtcg aaatcgttct tcagacgttc ctgcagcccc ggaatatccc acatttcttc
    11401 cagcgactgc ggcggaatgt aggcatcaat ggtggctttg aacacatctt cacgaatgct
    11461 gttgatggtt tcgctcacat cgctaacatc cagcagttcg ttacgctggg agtaaatggc
    11521 gcgacgctga tcgttagcca cgtcatcata ttccagcagt tgcttacgaa tgtcgaagtt
    11581 acgactttca actttacgct gggcgttggc aatcgctttg gtcacccacg ggtgttcaat
    11641 ggcttcgcct ggcttcatac ccagtttacg catcatgccg gatactcggt cggaagcaaa
    11701 aatacgcatc agcgcatctt ccatcgacag gtagaaacgg gaagaaccag catccccctg
    11761 acgaccagaa cgaccgcgca actggttatc gatacgacgg gattcgtgac gctcggtacc
    11821 gatgatatgc aggccacctg ctgccagtac cgcatcgtga cgtacctgcc agtcggcttt
    11881 aattttttca atttgctctg cggtcggatt ttccagcgcg gcaacttctg cctgccagct
    11941 accaccgagc acaatatctg taccacgacc cgccatgttg gtcgcgatag tcaccgcagc
    12001 cggataacct gcctgagcaa caatcgccgc ttcgttggcg tggaatttgg cgttcaggac
    12061 attgtgctta ataccggctt tggtcagttc gtttgacacc agctccgatt tttcgatgga
    12121 gatagtaccc accagcaccg gctggccttt cgcagtacgt tctttgatat cttcaatgat
    12181 cgcctgaatt ttttccgctt cagtcatgta gaccaggtcc ggcaaatctt tacgaatcat
    12241 tggacggttg gtcggaacaa cgacggtatc cagcttgtag atggagctaa attcgaaagc
    12301 ttcggtatca gcagtaccgg tcatccccgc cagtttttca tacagacgga agtagttctg
    12361 gaaggtgatc gaagccagcg tttggttttc gttctggatc tgcacacctt ctttcgcttc
    12421 cacagcctgg tgcagaccat cggaccagcg acggccctgc atggtacgac cggtgtgttc
    12481 gtcaacgatg ataacttcac catctttaac gatgtagtcg acgtcgcggg taaacagcgc
    12541 atgagcgcgc agcgccgccg ttacgtggtg catcagcatg atgttggccg gagagtacag
    12601 agactcccct tcatccatga tgccctcttt caccagcagt tcttcaatca gcaccagacc
    12661 acgttcggtc aggttcacct ggcgagattt ttcgtccacc gagaagtggc cttcgccctg
    12721 gaaggtttcg gagtcttctt tttcctgacg gatcaggtgc ggaataattt tattcacgcg
    12781 tttatacatt tccgagctgt cttctgccgg gccggaaatg atcagcggtg tacgcgcttc
    12841 atcgatcagg atggagtcca cttcgtccac cagcgcatag tgcagtttac gctgtacacg
    12901 ttcttcaggg ctgaacgcca tattgtcgcg caggtagtca aagccgtatt cgttgttcgt
    12961 accgtaagtg atgtcagctg cgtaagcttc gcgctttgcc ggtgctggca tgcccggcag
    13021 gttgataccg acagtcaggc caaggaattc aaacagcgga cggttgtttt cggcgtcacg
    13081 ttgcgccagg tagtcgttga cggtaactac gtgcacgcct ttaccggtta gtgcgttcag
    13141 gtaagcaggc agcgttgcgg tcagggtttt accttcaccg gtacgcattt cggcgatgca
    13201 gcgttcgtta agaaccatac cgccgagtaa ctgaacgtcg aagtgacgca taccaaagac
    13261 gcgcttactt gcctcacgta ccacggcgaa agcttccggg atcagatttt ccagcacttc
    13321 gcctttttcc agacgtgcac gaaactctgc ggttttccct ttcagttctt cgtcggagag
    13381 tttttccatc tccggttcca tggcattgat gatgttgacc actttgcgca tccggcgcag
    13441 ggtgcgatcg ttacgactac cgaaaacttt agttaacaat ttgattagca taataaaatc
    13501 tcaaacgccc cgcgttgcgg agttaataaa atgaagtaaa ggtttattgt tgttaggtga
    13561 ggcgttgagg gccagcacgg atgccttgcg cctggcttat ccagacgggc gtgctgaatt
    13621 ttgcttgtgg ggtaaaatgc gcataatcaa tgcgataacc cttttcagac ggcgtgcctt
    13681 cctgggtcag cagcgcgctg agcgtatcca gtaatgcaag atgttgcgcc tgaagaggca
    13741 aagattcttc agcaacgggc agtgtttgcg gtgccattgc gaaagaaaga tgacggatta
    13801 ccgtgcgaat ggcatgttga tgccagtaat caacggaata gttcgaattc gggcggcgtg
    13861 tgttcgcttc cagcaaggcc aattgaccaa agttaacttt ggctgaaggc tcgtggttgc
    13921 gggttgtcgc ttttgcgggc gcatttggtt cggcggcgtt gctgagcgca ggcaaaccta
    13981 aactcgccgc aaccatccct aataagagat gcggccagaa gtagcgttta ccaaactgtc
    14041 gccagcgcgt cagtattcca ctcacgttat tgccatcccg atcgtattca aacgtattta
    14101 gcgcacaaat cttatcatta aagcctgcag tccacagcac gaatgaagat aagttgtgcg
    14161 gaaaagtgct taagaaaaca gcgttcgcac cgttttttat cgttcttata aaattcaaaa
    14221 aaaaacgact ggttcacctg gccggagaga gtgccagatt tgccagtcga attttatacg
    14281 acagtataaa tgtcgttatg ccagtacggc tgaaggcgct ttgaaggcca acggcagttc
    14341 tgcgtcgtcc tggaaggtca catattccca ggcttcctgt tttgcaagga cagcctgcag
    14401 cagtttgtta ttcagtgcat gaccagattt ataagcggta aatgcaccaa taatattgtg
    14461 accacacatg aacaagtcac cgatcgcatc gagcattttg tgacgcacaa attcgtcttc
    14521 aaaacgcagg ccgtcttcgt tcagtacgcg ataatcgtca acaacgatgg cacaatcgaa
    14581 gctgccgccc aggcacaaac cacgggactg cagatattcg atatcacgca tgaaaccgaa
    14641 cgtacgcgca cggctgatct ggcgcataaa cgcatcagcg gagaagttca tcgcatagcg
    14701 ctggttgctg gaatcaatag ccggatggtt aaaatcgatg gtgaaatcca gcgaaaaacc
    14761 attgtacggc ttaaattcag cccacttatc gccatcttcg acacgaacag tctctttgat
    14821 gcgaacaaat ttcttggcgc agttcaactc gtcgataccg gcgtcaagca gcaggtatac
    14881 aaacggagcg gcactgccgt ccatgatcgg gatttccggc gcgttaactt cgataacaat
    14941 gttatcgatg cccaggcccg cgagagcagc attgaggtgc tctacggttg aaatccgtac
    15001 atcatgctcg ttgaccagac acgtacagag catggtatca cgcacagatt tggcatcggc
    15061 cgggaaatct accggtggat tcaagtcggt gcgacgatag atgaccccgg tgttggccgg
    15121 cgcagggcgt aacgtcaggg tgactttctt gccggtatgt aaaccgacac ccgtcgcctg
    15181 aacgatacgt ttaagtgtcc tttgtttgat catcgtatta tctcgccaaa ttacctatcc
    15241 aaccgaagtg tactatacat tcggcgggcc agtttagcac aaagagcctc gaaacccaaa
    15301 ttccagtcaa ttcttaatca gcttgcttac gcaggaatgc tgggatatcc agataatccg
    15361 gctctttcgc agtttgcggc gcattgtcat tcacgacttt agcaaccggc ttctgctcct
    15421 gggtcagcgg agccatcccg tgctgctggt agcgatccat cactggctgc tgaacctgct
    15481 tattggtcac cagagtgatt tcaggacgtt tgtccatgcc gatacctgtc gcaacaacgg
    15541 ttacgcgcag ctcgtcattc atatccgggt caagagaagt accgataacc acagtcgcgt
    15601 tgtcggaagc aaatgcacgg atggtgttac ctaccgtttc gaactcatcc agacgcaggt
    15661 cgaagcccgc cgtgatgtta accagcacgc cgcgtgcgcc agacaggtcg atatcttcca
    15721 gcagcggaga agagatagcc atttcagcag cttcttccgc acggtcttca ccgctcgcca
    15781 cgccagaacc catcattgcg tagcccattt cagacattac ggtgcgtacg tctgcaaagt
    15841 ccacgttcat caaacccgga cgagtaatca gttcggcgat accttgcaca gcgcctttga
    15901 gtacgtcgtt cgctgcgcca aacgcatcca gcagggagat accgcggccc agaactttca
    15961 gcagtttgtc gttcgggata gtgatcagag agtccacatg cttggacagt tcagtgatcc
    16021 cctgctccgc gaatgccata cgcttcttgc cttcaaagtt gaaaggctta gtgacgacag
    16081 caacggtcag gatacctaaa tcttttgcca cttcagcgac gactggtgct gcaccagtac
    16141 cggtaccacc acccataccc gctgcgataa agaccatgtc tgcaccttcc agcgccgcac
    16201 gcagtgcatc gcgatcctca tcagccgcat tacggccaac ttctggatta gcgccagcgc
    16261 ccagtccttt ggtgataccg ctaccgattt gaatcgtctg tccaaccgct gttttacgca
    16321 gcgcttgtgc atcggtattt accgcgaaga attcaacacc ttcaatgcgc tcgcgcacca
    16381 tgtgttcaac agcattaccg ccgccgccgc cgacgccgat gactttaatc accgcgtcat
    16441 tggtaagttc cattggttca aacatagttt ctctccgatt tgtgcctgtc gcctgaggcc
    16501 gtaatcatcg ttggcctcat aaaaattaaa actcttttcg cagccaacta ttgagtcgct
    16561 tgatccacga gccaactgat gctgtaacac gtttttctac ttcagcttca ccgttaagat
    16621 gtgactcttt cccatagtga agcaatccca ccgccgtcga ataatacggc tcctgagcat
    16681 aatccgttaa accggtaatg ttcagcggcg cgccgatacg cacttgcgta tgaaacacgc
    16741 gctgagcaca ggctgcaaga ccttcgatct gcgctgcgcc accagttaaa acaatgcctg
    16801 ccgccaggtg atgtttaacc ccttgttggc gaagcttttc ctgcaactgc aatatctctt
    16861 cgttgaccag gttgagcagc tcggtatagc gcggctcgat cacctctgcc agtgtctgac
    16921 gttgcagact ccgcggtgga cgaccgccta cgctcggcac ttccacgctc tcatcttttc
    16981 caacgatgga acccagcgca caaccgtggc gaactttaat cgcttcggcg tcgcttggcg
    17041 gcgtgccaaa ggcgtaagcg atgtcactgg tcacgacatt gccagcataa ggaattacct
    17101 tagtgtggcg caatgcccca ccggtataaa cggcgatatc cattgtacca ccaccgatat
    17161 cgacgacgca gacacccagt tcacgttcat cttccgtcaa taccgaataa cttgatgcca
    17221 gtccggcaaa tatcagttgg tcaactttca gcccacaacg ttcaaccgct ttgacgatgt
    17281 ttttcgccat atcgttgtga catgtgatca ggtgcacttt tgcctgcatc cgcacgcccg
    17341 aaagtcctac cggattcttg atcccttcct gatagtcaat cgcatactct tgcgggatca
    17401 catgcagcac acggtgttca tcgcgcacac gcaccgattt cgcggtatgg acgacgtttt
    17461 ccacatcttc ttgcgtcact tcttcttcag aaataggcac cataccaatt tcattctggc
    17521 agctgatgtg cttaccagaa agcgccagat ataccgaaga gatctgacaa tctgccatca
    17581 attctgcctg gtcaatggcg cgttgtacgc acttgaccac ggattcgagg tcgttcaccc
    17641 cgcctttatc cataccacgc gacgggcagc tgcccacgcc aatgatattg accataccgt
    17701 cgggcagaac ttcccctact aaagcggcaa ccttcgcggt accaatctcc agtcctacta
    17761 ccagttttct gtccgtcgcc ttgatcattg ttgttctgcc tgtgcctgat tttgttgctg
    17821 agtagattcc tctggcggca agggcgccca gcctactgcc gctccagagt cataacgcaa
    17881 atcaacgtag ctaatccgtt tgccatcggt ttgcgcctgc tgctgtaaaa ccggataaag
    17941 ttctacaaag cgagccaaac gtttcatcgt atcgccccgg ccaagattga gcttaatatc
    18001 gttattcagc gtcaactgcc aggaacgccg cgcggtcatc gccgcttcct tcagagtaaa
    18061 tctgtccttt gccagcatct gccccatttc gcgatagccc tgcaacactt cattggcgct
    18121 gccttccggg ccatacagca ttggaagcac ctgcttgctg gtgcgatctg gcggcacgct
    18181 gaaagtgttt ccttccgcgt ctaccatatg ttgatcattc caccgcgcaa tcggcacata
    18241 ttcaaccaga tgaatcttca attcatcagg ccactgcttt ctgacgctca cctgcttaat
    18301 ccacggcagg cgttgttcta tttgcgtctg gatgatgttg acatcctggg tcataaaggt
    18361 acctggctca cccaatgcca ggatcgactg ccggatatcg tcattacgcg tgtaatggcg
    18421 ttcaccggtc aacaccagct ttgagagcgg caggcgttgc gcatcttcca tccagcccaa
    18481 cacgacccag ccgctcacca acactgtcgt taaaacggtc agcaggaaaa ggatccccgc
    18541 cagacgcgtt ccattattgc ggcgagaaga aacctcttct tcgctgtttc gcgtgttcag
    18601 agcagcctgc gacatattag tccgccagtt ccagaattcg tactaccaac tgcgagaagc
    18661 tcatacctgc ctgacgtgcc gccatcggca ccaggctgtg gctggtcata cccggtgagg
    18721 tattggcttc cagcagataa aactgtccat cgctgtccag cataacgtca atacgtcccc
    18781 atcctttgca acctaacgtc gtccatgctt tcagcactaa tgcctgcaaa ttggcctctt
    18841 gtgacgcttc cagacctgcg gggcagaaat actgtgtctc atcagagaga tacttcgcct
    18901 cataatcata gaaggttccg gacggttgaa tacgtattga cggtaaaatt tcttcaccga
    18961 gtatcgcaac cgtgaactcc ggcccactta gccatttttc aatcaatact tcttcatcgt
    19021 gctgaaatgc caatcttaat gcatcttgta gagcattttc tgctactact tttgacattc
    19081 ccacactgga accttcgcgg ctcggcttaa cgataaccgg caaacctaag gcagaaattt
    19141 ctgctaactg cttatcgctc aggccttttt caaactctgc gcgggttaat gctacccacg
    19201 gcgctaccgg taaaccagcg ccctgccaaa gcaatttgct gcgtagttta tccattgaaa
    19261 gcgcagatgc catcactccg ctgccggtat aaggcaagcc catcagctcg agcataccct
    19321 gcagcgtgcc atcttcaccg ccgcgaccat gtagcgcgat aaacactttc tgaaagccca
    19381 tcgacttcag ttgcgtcacg tcgacttctt tcgggtcgac aggatacgcg tcaataccgc
    19441 cttcacgcag cccggctaac accgctgcac cagaattcag agaaacttcc cgctcagcgg
    19501 aggtcccgcc caacaggacc gcgattttat cagtcatgtt gttcttcctc cggagtttgc
    19561 ggcttcagtt tgatttcagc taaagaacgg gcaatttttc cgatattacc agccccctga
    19621 acgagaatca ggtcgttacc ggttaatacc ggtgccagca tctcggctac ctgcgccggg
    19681 tcaggcacca gaatgggatc aattttccca cgtccacgaa ttgtgcgaca cagcgaacgg
    19741 ctgtccgctc ccggaattgg cgcttcgcca gccggataca cttccagcat caacagggta
    19801 tcaacctgcg tcagcacatt ggcgaaatca tcatacaggt cgcgtgtacg ggtaaaacgg
    19861 tgcggctgaa acagcattac caggttttta tccggccagc ctgcacgcgc cgctttaata
    19921 gtggcgtcca cttccgtcgg gtggtggccg tagtcatcga ccagcatcgc cgtaccgctt
    19981 ttaccattca ctggctccag cgggaattca ccgaggaaat caaaacggcg accagtcccc
    20041 tggaagctct ccagcgcacg caaaatagcc tcgtcgtcaa tgccctcttc cgtagcaacc
    20101 gcaaccgcag ctgcggcgtt cagcgcgtta tgacgacctg gcgcatttag ggtgacgcgc
    20161 atcggctctt tgtcctggcg cagcagcgta aaatgcccct gcgggccaat ctgctgataa
    20221 tcttctacac gcacgtcggc atcttcgctg aagccgtaag tcgtggtctg acgccccaca
    20281 cgcggtaaca attcgcggat caccggatca tctacacaca tcaccgcacg accgtaaaac
    20341 ggcaggttgt gcagaaaatt aataaaggtc tgttttaaat tctcaaagtc gccctggtag
    20401 gtatccatgt ggtcggcttc gatattggtg acaatcgcca ccatcggttg cagatgcagg
    20461 aacgatgcat cactctcatc tgcttcggca atcaggtacc gaccatgccc caaacgcgca
    20521 tgaacccccg ccgcttttac cagcccgccg ttaacgaagg ttgggtcgag ccccgcttct
    20581 gcgtagatgc tggagaccat cgcggtagtt gtcgttttgc cgtgcgttcc tgcaatggcg
    20641 atgccatgac gaaaacgcat taactcagcc agcatttcgg cacgacggat caccggaata
    20701 cgcgcttcat gagctgcgac aatttccggg ttatcggcag aaatggcgct ggaaacaacg
    20761 accacgctgg catcacgtac gttttccggg cgatggttga aataaatcgt cgctcccaga
    20821 ttcattaact gctgcgtgac cggatttggc gctaaatcgg aaccactgat ctgataacct
    20881 tcattggcca gaacttcggc aataccgccc ataccggcac caccgatgcc gacaaaatgt
    20941 atgtgccgaa cgcgacgcat ttcgggcacg atggaacgca gttttgccaa ttgttgtgta
    21001 ttcattgttt acgccattaa cttcttaaat tcatacgatg caaaaggcat cgctacaatt
    21061 acgcccgggc agcccggctc acttcatttg ccactcgctc ggtggcatcc ggaatggatg
    21121 cagcgcgggc gcgttctgcc atggttaata aggtttctcg cgaccacccg gccagggtgt
    21181 tggcgacagc atccacgcta agctgtggct gctcgataat tttggctgcg cccgcttttt
    21241 ccagcggtag cgcattccag tattgctggc ggtctttatg ttgaaacggc acaaacaacg
    21301 ccggtagtcc tgccgcagcg atttcactca ccgttaacgc accggagcgg caaacgacga
    21361 catccgccca cgcatacgcc gccgccatat catcaataaa ttccgtcact ttatgctgcg
    21421 gttgccccgc ttcggcatac gcctgttcaa cggattgttg cgaacctttg ccgctctgat
    21481 gccagatagt gactgaatca cccagtttcg cagcaacctg cggcattgtc tggttaagaa
    21541 tgcgtgcgcc ctgggaacca cccactacca acacacgaac cggaccttca cgtccagcca
    21601 aacgttgctg cggcaacggc agcgccagca catcggtacg caccgggtta cccaccactt
    21661 ccgcattagg gaaagcgcct ggaaacgcct gcatcacttt ggtggcaatc ttcgccagcc
    21721 atttattggt taagcccgca ataccgtttt gttcatgaag tacaaccgga atgcctaacg
    21781 accacgcggc cagaccacct gggcctgata cgtagccgcc cattccgagc accacgtcag
    21841 gtttgtacgc tttcataatc gcccgcgcct gacgccaggc gttgaagatt cgcagtggcg
    21901 cagctatcag tgcttttata ccttttccac gcagaccaga gatacgaatg aaatcaattt
    21961 cgatgccatg ttttggcact aagtccgctt ccatacggtc ggcagtcccc agccagcgaa
    22021 cttgccaacc ctgagccatt agatggtgcg caaccgccag tcccgggaat acatgtccac
    22081 cggttccgcc tgccatcacc attaatcgct ttccttgacc actcatcgtg aacctcgtac
    22141 aaacgcctgc gctttctcca gacgcgtttc ataatcaata cgcaacagca tcatgatggc
    22201 tgtcgacata atcagtaagc tcgaaccacc gtaactgatc aacggcaatg tcagaccttt
    22261 ggtcggtaac atccccgccg ccgcgcctac gttaaccagc gcctggaagc taaaccagat
    22321 gccaatggaa caggcgagaa aaccggaaaa acgatggtca atttctaatg ctttacggcc
    22381 aatcgacatc gcacgaaaag cgacgaagaa taccattaaa agtgctagta ccacaccgac
    22441 ataacctaat tcttcgccga taatggcgaa aataaagtca gtgtgcgctt ccggcagata
    22501 ctccagtttt tgtaccgagt tacctaagcc ttgcccccaa agttcgccgc gaccaaacgc
    22561 catcagcgat tgcgttaact gataaccgct gccaaaggga tcttcccacg ggttccagaa
    22621 tgcggtaaca cggcggatac ggtacggttc ggcgaggatc agcaacacaa ccgctgaaat
    22681 gcccataccg ataatggcaa tgaactgcca caatttcgct cccgccagga ataacatcgc
    22741 cagcgtagtc acaaacaaca ccaccaccgt accaaggtct ggctgtgcca gcagtaacac
    22801 tgccaacacc agaatcacgc ccatcggttt caggaagccg cgcaggttat tacgtacttc
    22861 gtcaccttta cgcaccagat agttggcgat atagcaaaac agcgacagtt ttgtcagctc
    22921 cgcaggctgg atacgcagca aaccgagatc gatccaacgc gatgcccctt taacagagct
    22981 acccactacc agcacgatca tcagcaggat gattgatccg agcagcatcg tggcactgta
    23041 gcgttgccag aactccatcg gcagacgcag cgtaatgatc gccagaatga atgccaggat
    23101 cagatagacg ccatcacgct tcgcgaagaa gaacggatcg ttggttaagc gttgccctat
    23161 gggcattgac gccgaggtca ccatgataaa gccaatcgcc gcgaggccga aggtcagcca
    23221 cagtaaggtg cgatcgtaca tgatcaggct gtcggtatct ttttcccgcg agcccatcac
    23281 ccagcccttt agcgccgtgg agatccagac cagaatactg aatcctggca ggcgcggcat
    23341 tttcaggcga gggagagata aacgcatcaa cctaactcct tcgccagacg ggcaaactca
    23401 ttgcctcgtt gttcaaagtt cttgaactga tcaaggctgg cacaggccgg ggagagcaga
    23461 accatatcgc ccggctgaac acgcggagcc agcaagcgca tcgcctgttc catagtttcg
    23521 gtttgttctg ccacttccgg gcgtagcgcc gccagctgcg cgccgtcacg accgaaacaa
    23581 tacagacgta cgttatcgcc attcaggtaa cgcgccagtg ggctaaaatc tgccgattta
    23641 ccatcgccgc ccagcaacaa atgcagcgtg ccgtctacgt gcaggccatt cagtgccgct
    23701 tccgtactgc cgacgttggt cgcttttgaa tcgttaatcc agcgcacacc gttatgctcc
    23761 agcacaactt caaagcgatg cggcagacca gtgaatgtgg ttaacgcttt caggctgctg
    23821 gcgcgcggca aaccagcggc atctgccagt gccagagctg ccagtgcatt ggtgtagtta
    23881 tgctgcccgg aaagtttcat ttctttcacg ttcagcactt tctcgccttt cacccgcagc
    23941 caggtttcac cctgctgatg attcaggtga tagtcaccca tgttgacgcc aaagctgacg
    24001 cagcgttcat ccgcaccgcg aatcggcatt gttaaggcat catcagcatt aaccacgcaa
    24061 atttttgcgt tttcgtaaat gcgcagtttt gctgcacgat actgttgtaa accaaacgga
    24121 tagcgatcca tatgatcttc agtcacgttc agaatggtcg ctgctaccgc ctgtaaactg
    24181 gaggtggttt ccagctggaa gctcgacagc tccagcacgt acagttcgca ctcatcatcc
    24241 agcagcatca acgcaggcag gccaatattg ccacccacac caacgttaac ccccgccgct
    24301 ttcgccattt cacccactag cgtggtgacc gtgcttttgc cgttagaacc ggtgatcgcc
    24361 acaatcggtg cttgtgcttc acgacagaac agctcgatat cgccaacgat ttcgattccg
    24421 gcatcagcgg cagcgcttaa ggatggatgc gccagtgcaa taccgggact ggcgacaatc
    24481 agatcggccg ccatcagcca ttcatcattc agaccgcccg tgtggcgttc tacagcttcg
    24541 ggtaatttat ccaggccagg cggtgtcata cgcgtatcca taacgcgcgg cgtcacaccg
    24601 cgagcgagga aaaagtccac gcaggaaagc ccggtgaggc ccaggccgat aatgacaaca
    24661 tttttaccct ggtaatcagc catgattaac gtaccttcag cgttgccaga ccaatcagga
    24721 ccagcatcag cgaaataatc cagaaacgca caatgacgcg cggttccggc cagcctttca
    24781 gttcatagtg gtgatgaatc ggtgccatgc ggaaaatacg ttgtccgcgc agtttaaagg
    24841 agccgacctg caggatgacg gaaagcgttt ctaccacgaa cacgcccccc ataatcacca
    24901 gcaggaattc ctgacgcagc agtacggcga taatacctaa cgcaccacct aatgccagcg
    24961 aaccgacatc gcccataaag acctgcgccg gataggtgtt aaaccacagg aagcccagcc
    25021 ctgccccgac tatcgcggta cagacaatga ccagttcccc ggcgtgtcgt agatacggta
    25081 tgtgtaaata gctggcaaag ttcatgttgc cggtcgccca cgccaccagc gcaaaaccac
    25141 cggcgacaaa tacggtcggc ataattgcca gaccgtcgag accatcagtc aggtttaccg
    25201 cgttgcctgt acccacaatg acgaagtaag ccagcagaat gtagaacagc cccagttgcg
    25261 gcatcacatc tttaaagaac ggaaccacca gctgcgttgc gggcgtgtct ttgccggcaa
    25321 ggtacagcgc gaaggcaaca cccagcgcaa tgaccgacat ccagaaatac ttccaacgag
    25381 cgatcaaccc tttggtgtct ttacgcacca ctttgcgata gtcatcaaca aagccaatga
    25441 tgccgtaacc taccagcacc accagtacgc accagacgta cggattggat ggataagccc
    25501 acagcagcac ggagatcaca atcgccgtca ggatcattat cccgcccatg gtcggcgtac
    25561 cgcgcttact aaagtgcgat tccgggccat cgttacgaac gacctgacca aaggagagtt
    25621 tttgcaaatg ggcaatcata cgcgggccca tccacaatga gatgaacagc gcggtcagca
    25681 ggctgacgat ggcgcgaaac gtcagatagg aaaagacgtt aaagccggaa taatatttga
    25741 ccaaatgttc ggccagccaa actaacatgt cccattctcc tgtaaagcgc gtactacctc
    25801 ttccatggcg gcactacgtg aacccttaac taaaatcgta attacctgtt gctcagcaat
    25861 cagtgattta agacgcgcga taagcgcagt tttatcggta aaatgttcgc caacaccgct
    25921 ggcagtgctg atagcatggc tttgtttacc cacacttaac acgcagtcaa taccagcagc
    25981 ttttgccgct tcgcccacct gtacatggca ggcttcgctt tcagcgccca gttccgccat
    26041 atcgcccacc accagcacgc ggtagcccgg catttcagcc agaacctgaa cagcggcggt
    26101 cattgaaccg acattggcgt tgtaggagtc gtcgagcagc aactggtttt ctgccagttt
    26161 tatggggaac agacggcctg gaacagcttt cagatttgcc agccccgctt tgatagcatc
    26221 aagcgttgcg cccacggaca tggagagcgc agcggctgcc agcgcattcg caatattgtg
    26281 acgccccggc aacggcagca gaacatcgac gctacctgtt ggggtttgca gggtaaattc
    26341 cgtaccgtgc gaagtcacat ggatattggt ggcggtgaaa tcgctgttgg cggcattggg
    26401 tgagaaacgc cacactttgc gtgagccaat tacgctctgc cagttcagcc agtcgttgtt
    26461 gtcagcgttc atgatggcga taccgttttc cggcaggccg ctaaatattt cacctttcgc
    26521 tttcgcgaca cccgcaagcg agccaaaacc ttccagatgt gccgctgcca ggttgttgac
    26581 cagcgccgct tccgggcgag tcagactcac agtccaggca atttcaccct gatggttcgc
    26641 gccaagttca ataactgcgt aatcgtattc cggcgttaag cgcaacagcg tcatcggtac
    26701 accgatgtcg ttattgagat tgcctgccgt ataaagtgtg ttgccgcact ggtttaaaat
    26761 tgccgccgtc atctctttaa cggatgtttt gccggaggaa cctgtcagag caaccacgcg
    26821 cgccggaact tgctggcgaa cccatgcagc cagttcacca aacgccagac gcgtatcctt
    26881 gacgattaac tgcggcagat cgatatccag cggacggcta accagtagtg cgcctgcgcc
    26941 gccagctttc gcctggtcgg caaaatcatg agcatcgaaa cgttcgcctt tcagggcaac
    27001 aaacaggcag cccggcgtca gttttcgcgt gtcagtggtt acagcatcaa gggtaatatc
    27061 tgcaccttgc agttcaccgt tgagaatatc ggtaagttgg ctaagggtta cgctaatcat
    27121 gcaatcaccc ccagcagacg cgccaccgtg acgcgatcgg agtagtccag acgctgattg
    27181 ccaacaatct ggtaatcctc atgacctttg cccgcgacca gtaccacatc attctcttta
    27241 gcctgcataa cggcgcaagt caccgcttca gcacggcctt ccatcacttt ggcatgtccg
    27301 gcatctaaca ttcccgccag aatatcgttg atgatggcac gcggttcttc ggtacgcggg
    27361 ttatcgtccg ttaccaccgc cacgtcagca aactcttcag caattgcgcc catcagtgga
    27421 cgtttgcctt tatcacgatc gccaccacag ccaaagacgc accacagctt gcccgcacag
    27481 tgcaggcgcg ccgcctgtaa ggctttttcc agtgcatctg gcgtatgcgc gtaatccacc
    27541 accaccgtcg gtttgcctgg tgcagtgaac acttccatac gtccgcaaac cggttgcaga
    27601 cgcgcggcgg ttttcagcag ctcagccagt ggatagccca gcgccagcag cgtcgccagc
    27661 gccagcagca ggttgctgac gttaaaagcg cccatcagat ggctttcaat ttccccatcg
    27721 ccccagcttg agctaaagcg aatagtcgca ccgctgtcgt gatagttcac ttcagtcgct
    27781 ttcaaccagc gtccgtgaca gttcggattg atatgatctt ccattgatac cgcaaccgcg
    27841 tccggcagtt ttgccagcca gcggcggccc acttcatcgt cggcgttaat aatcgcctga
    27901 ccgcaatgat gctcagaata aagcagccat ttcgcggctt cgtagtgttc catatcacca
    27961 tgataatcaa ggtgatcgcg gcttaagttg gtaaagaccg atgccgcaaa tttcaatgcc
    28021 gccacacggt gctgtaccag tccgtgggag gaaacttcca ttgcgcaaaa agtcgcgccc
    28081 tgatccacca gccccgccag ctcatgctga acatcgactg ccgaaccggt ggtattttct
    28141 gtcgggatca ctttccccag caggccgtta ccaacggtgc ccattaccgc gctggtttcg
    28201 ccaagcagtt ggctccactg tgccagcagc tgggtcgtcg tggttttgcc gttggtgccc
    28261 gttacgccta caagacgtaa attgtcagag ggttcatggt aaaagcggcc cgccagtgca
    28321 gataaacgct cgttgagctg gctgagatag atgaccggta cgccgtgcat ttcacggatt
    28381 tcaccgtcgg tcgcctcatc tttcgcctct gcaataatgg cagccacacc ttgcgctatc
    28441 gcctgcggga tatatcgacg cccgtccgcc tgatgaccta ctacagctac aaagagatcg
    28501 cccgccgcag ccacacggct gtcgagtgtc atctctcgca gtgctcgcga aggtgcgtct
    28561 ggcacccacg gagcaagaag gtcgcgcaaa ttacgatctg ccacctgtcc cctcgccttg
    28621 attaatcaca aattcatttt tatcgcccgt tgtcagcgca tccggctcga tgttcatggt
    28681 acgcaatacg ccgcccatga tggcaccaaa gaccggcgcg gaaacggcgc cgccgtagta
    28741 tttacccgcc tgcggatcgt tgataacaac aaccagcgcg aagcgcggct gactcgcagg
    28801 cgcaacgcct gcggtataag caatatattt attgatgtag cgaccgtccg gcccgacctt
    28861 tttcgcggta ccggttttaa tggcgatacg atagccttta atcgccgcct tcacgccgcc
    28921 gccgcccggt agcgccacgc tttccatcat atgcaccacg gtgcgaacaa tggattccgg
    28981 gaagacacgt tcaccgggaa ccggggggtc aactttggta atcgacagtg ggcgataaat
    29041 gccgtagctg ccgatggttg cgtagactcg cgctaactgt aacggtgtta ccattagccc
    29101 gtagccgaaa gagaaggtgg ccctctctat gtcagaccac cgttgttttt gaggatataa
    29161 gccactgcgt tctccgacca accccaaatt ggtcgctttt cccagtccaa aacgtgagta
    29221 agtatctact aacgctgagg acggcatcgc taacgccagc ttggaaacac cgacgttact
    29281 cgacttctgt aataccccgg tcagggttaa ttcgctgtag cgtgccacgt ctttgatttc
    29341 gtggccgtta attcgataag gaacggtatt cagtaccgag ttttcccgca ctacgccacg
    29401 ttgcaacgcg gtcatcacca ccatcggttt aaccgttgaa cccggttcaa acacgtcagt
    29461 gatggtacgg ttacgcatcg cctctttcgg cgtaccactc agattgtttg ggttgtatga
    29521 cgggctgtta gccatcgcca gcacttcacc ggtgttgaca tccaccagca cggcgctacc
    29581 agattcagcc ttgttaaagg ccaccgcgtt gttcagttcg cgataaacca gcgcctgcag
    29641 gcgttcatca atactcagcg ccaggttgtg cgccgcctga ctgtcggtgg aagagatatc
    29701 ttcaattacg cgaccatagc ggtctttacg cacaatgcgc tcacccggct gcccggtaag
    29761 ccatttatcg aaactcttct cgacgccctc aatcccctga ctatcgacgt tagtaaagcc
    29821 gatgaggtga gcggtcactt cgccggatgg ataataacgg cgagattctt cacgcagatg
    29881 aatccccggc agtttcagtt ttttgatgta ctctgccatg tcagggttta cctgacgcgc
    29941 cagataaata aagcgccctt tcgggttggc gttaatgcgg gctgaaagct gatccagcgg
    30001 aatgttgagt gcgttagcca gcgccttcca gcgatcaccg acgctgatac cgccagcgtc
    30061 atgcacttct ttcgggtcag cccaaatcgc tttaaccggc acgctcaccg ctaacgggcg
    30121 accagaacgg tcagtaatca tgccgcggga ggtggaaact tgctgaacgc gaagagaacg
    30181 catgtcgccc tcttttacca gcatatccgg ggagataact tgtaaccacg ctacgcgtcc
    30241 gagcagaaaa gccagcgcca ggagaataca gccgcataac aacgcaaaac gccaactgat
    30301 aaagttggca tgttcttcct gacgttttgg tttctgcgtt ttcgccgctg ctttcatgcg
    30361 tcgcgtttat ccttattttt gcactacgat attttcttgt gacggatcaa catgctgcat
    30421 ttgcagcttt tccgtggcga tcctttccac ccggctatgg tcgccgagcg cattctcttc
    30481 aaggatcagg ttgcgccatt caatgtctaa agcatctcgc tccagcacca gttgttcgcg
    30541 ctgagcggtc agtaaacggg tatggtgcgc cgtggttacc acggtcaccg ccgtcaaaat
    30601 aatgcaaatg aacaggcaga gtggcagctt cccaaatcgc aaaagatcgt caccgataac
    30661 accaggcaat gcatggcgct cgtggcttcc catcgatcct ttaactttgc ttagagcttc
    30721 tgtcactctg ctgatcatgc gttcgtcctc tctgcaatac gcagaactga actacgggca
    30781 cgagggttct cagccacctc ttcttcgccc ggcattaact tgcctagtgc tcgcagctga
    30841 cggccaccca gttttttgag ctgctcttca gtcatcggta accctgccgg aacttgcgga
    30901 ccgcggctgt tttcacgcat aaaacgtttc acaatacggt cttccagcga gtggaagctg
    30961 atgatcgaaa gccgcccacc cggggccagc acgttgagcg agctttttag cgcctgctct
    31021 atctcctcca gttcactgtt tacccaaatg cgcaccgctt ggaaggtccg ggtcgcggga
    31081 tgtttaaact tatctttcac cggcgttgca gcagccacga cttccgccag ttctttggtg
    31141 cgggtcatcg gctgttcgcg gttacgctcg acaatggcgc gggcaatgcg tttggcaaaa
    31201 cgctcttcac cataggtttt caatacccag gcgatatcgg cttcttctgc ggtttgtagc
    31261 cattcagcgg ctgactgccc acgggttggg tccatacgca tgtccagcgg accatcgcgc
    31321 ataaaggaaa aaccgcgttc agcatcatca agttgcggtg aagagacgcc aagatcgagg
    31381 agaatgccgt cgatcttgcc gataagatcg cgctcggcaa cgtaatcgcc cagcgcggag
    31441 aaaggtccgt ggatgatgga gaagcgcgga tcatcaatag tcttcgcaac ggcgatagcc
    31501 tgcgggtcgc gatcgatcgc caacaaacgc ccctcttcgc caagctgcga gaggatcaga
    31561 cgtgagtgac caccgcgacc aaaagtccca tcaatgtaga tgccatcagg acggatattg
    31621 aggccattaa cggcttcatc cagcagcacc gtagtatgtt tatagttttc catcatttta
    31681 tagagacaag tcctgcagtc gctccgataa gtctccagta gccaactgct ctgcgtcgat
    31741 atcttccttg acctgttgat gccaggttgt ttcatcccac agctcaaact tgttgaactg
    31801 tccaaccagc atcacttctt ttgtcagccc ggcatgttgc cgcagtactg gcgcgattaa
    31861 caatcgacct gcgccatcca tctgacattc gctggcatga cctaacagta ggcgctgcac
    31921 acggcgctca accgggttca tgctcgacag acgcgataat ttttgctcga taatttccca
    31981 ttcaggcagg gggtaaagca gcaggcacgg gtgatgaatg tcaatggtgc aaaccatttg
    32041 accggcagcg ttctcaagca gctgttcccg ataacgggta ggcactgata agcgcccttt
    32101 gctgtcgaga ttgactaacg ttgctccccg gaacatgcca gcctcacccc ttattcccac
    32161 tttgccccac aatttcccac tgaaaggagt ttacggagcg gaggaaaagc ttgtcaagcc
    32221 agtcacaagg cataccgagg taaaaaaccc gatgtttacg gctaatatca gcgttgttta
    32281 ttttacgtcc aacgtgcgaa gcaaattaac gttatgaata gctgtaagaa aaaatccctc
    32341 atcgtcatca ccgttttaaa atgactcact attttccaca gcaaatataa agtgtcagtt
    32401 tgcgacgcga gcggcatttt aggacatatc ttccccggtt aacagtccct gttgcgtctg
    32461 tgtggcgctt gatgccagcg ggtttgcgca aaagcaaaga aaagtgtttg ttaaatcggc
    32521 aattcatctc tgcatgtaac aaaataatgc aaaaacgcgc tatcgatatc acttaattat
    32581 tgtgaatttc agctggtgag gtaattaacg ggcaggttta aaagcattta tcaataatag
    32641 ccaggaatag tcttatttac tttaaggata ttgatgtcca ttcccgtact caacgcgcca
    32701 gagagaaatt cacctggcgc gtatttttgt tcgcggctta gctacggctg agcacgccgc
    32761 ggcgatagag attacgttta atgcgcgtta aaccaggttt tggcttacgc ggttcgtcca
    32821 ggcttgccag gacaatctcc agcacacgct ctgcgacatc gcggtgacgt tgagccactg
    32881 ccagcaccgg gcactgtaag aagtcgagca gttcgttatc gccaaaggtg gcaattgcca
    32941 ggtcagaagg cagtttgccg tcacgacgta gcgtgacatc catcacccct tgcaacaacg
    33001 caaacgacgt tgtgaacagt gcctgcggca tcggatgcgt ttccagccat ttttcgaata
    33061 actgggcagc tgcctcccgc tcatagctgt tggcataaag gaaatgcact tcgcgcggat
    33121 catctttcca ggcagtacgg aagccttgtt cacgcaggaa gctgacagaa agctccggca
    33181 gcgcgccaag ataaagcacc gtctcggcgg gaaacttacg taactcttcc gccagcattt
    33241 cggcatcatc ctgatcagca ccaaccacgc tggtgaagtg ttcacgatcg agggcgcggt
    33301 ccagcgcgac aatcgggaac gggtcgttag cccagcgttg ataaaaagga tgctcaggtg
    33361 gcaacgacgt cgaaacaata atggcatcaa cctgacgctg taaaaggtgc tcaatgcagc
    33421 gcatttcgtt gtcgggctga tcttccgagc aggcaatcag cagttgataa cccctttgcc
    33481 gcgcctggcg ttcaagatag ttagcgatgc gggtatagct ggtgttctcc agatcgggga
    33541 tcacaagacc aatagaacgt gtgcgtccag cacgaagccc agctgccacg gcgttcgggt
    33601 ggtaattgtg ctcacgcacc acagccatga ctttttcaac ggttttgtcg ctcacacggt
    33661 attgcttcgc tttgccgtta ataacatagc ttgcagtggt ccgcgacact cccgccagcc
    33721 gagcgatttc atccagtttc acaattgccc cttgcgtaaa atgtaaaaac catagccatt
    33781 gtactggtat gggttaaatt cattaacatc taagcgcaga aaattaactg cggcaactgc
    33841 ttttattcca ggttcccact gatttcgcaa aaaaagccca acgtgacatg ttgggctgca
    33901 aattgcgcat tgagatcatt ctcaacgcat tattttttcg ccgcgcgaaa gtccgaccac
    33961 accagagcga gcaacttcca caattttcgc cacatcgcga atcgatgcta aaaatgcatc
    34021 aagtttatcg ctggtacctg ctaattgaac ggtataaagc gagggtgtga catcgataat
    34081 ttgcccacgg aatatttccg tattacgttt cacttcgtca cgcccgtagc cgctggcctg
    34141 aattttcacc agcatgattt cccgctcaac atgcgcaccc tgccccaact cgctcacgcg
    34201 caagacatcg accagcttgt gtaattgctt ttcgatctgc tcaagcactt tttcatcgcc
    34261 tacggtctgg atggtcatac gcgataatgt cggatcgtca gttggtgcaa cggtcaggct
    34321 ttcaatgttg tagccacgct gggaaaaaag gccaatcacg cgggataacg cgcctgattc
    34381 attttcgagt aagactgata atatccggcg cataatcagg ttctctccgt tttgcttaac
    34441 cacatttcat ccattccgcc cccgcgaatc tgcatcgggt agacgtgctc actgccatcg
    34501 acggtgacat caacaaacac caggcgatta ttgcgcacct gttccagcgc ctcgctaagt
    34561 ttgctttcca gttcttgcgg atgagaaatc tggatcccga cgtgaccata ggcttccgcc
    34621 agacgaacga aatcgggtag tgactgcata taagattgtg aatgacggcc ggagtagatc
    34681 atgtcctgcc actgtttcac catccccaga tagcggttat tgagattcac caccagtacc
    34741 ggcaactcgt attgcaacgc tgtagacagt tcctggatgt tcatctgaat actgccgtcg
    34801 ccagtgacgc aaaccacggt ttcttctggc aacgccattt tgacgcccag tgccgcaggt
    34861 aaaccaaaac ccatcgtgcc gaggccgcca gagttgatcc agcgacgcgg tttgtcgaat
    34921 ggataataaa gcgcagcaaa catctggtgc tgcccgacat cggacgtcac gtaagcgtct
    34981 cccttcgtca accgccaaag agtctcgatc accgcctgcg gtttaatctt ttcactgtga
    35041 gagtcatatt tcaggcactg acgagcgcgc cactgttcaa tctgttgcca ccagtcgcgg
    35101 atctcatcca gcggttgatg gacggattct tgcgacagga gttcaagcat ttgttcaagg
    35161 acctggcgag catcccccac aatcgggata tcagcagtca cggttttaga aatggaagta
    35221 ggatcaatgt cgatatgcag aaccgtggca tttgggcagt acttcgccag attgttcgtc
    35281 gttcggtcat caaaacgcac cccgacggca aaaatcacat ccgcgttatg catcgtcata
    35341 ttggcttcgt aggtgccgtg cattcccagc atgcccagtg cctgacgatg cgttgccgga
    35401 aacgccccca gccccattaa tgaggaaaca acgggcagat tcaacgcctc caccgtttct
    35461 tttagctgct gatgacagcc cgccgtgatt gccccaccgc ctacatagac aaccggtttt
    35521 tttgccgcca ccagcgtttg cagagcgcgc ttaatttgcc ctttatgccc ggaggtcgtg
    35581 ggattgtaag atcgcatact gaccgactcc ggccagacgt agggtaattt gttcgccgga
    35641 ttaagaatat ctttgggtaa atcaacgacc acgggcccag ggcgaccact tgccgccagc
    35701 cagaaagcct ttttcagcac ctgcggaatg tcttccgttt gcttaaccag aaaactgtgt
    35761 ttaaccaccg ggcgagaaat ccccaccatg tcgcactcct gaaaggcatc gtagcctatc
    35821 aacgaggtcg ctacctgccc ggaaaggaca actaagggaa tggaatccat ataagcggtg
    35881 gcaatgccag taatcgcatt ggtcgcccct ggacccgacg ttaccagcac gacgccgact
    35941 tccccggtcg cgcgcgccag accatcggcc atatgcaccg ccgcctgctc atgacgaact
    36001 aaaacatgat cgataccacc cacggtatgc aatgcatcgt aaatatcaag gaccgcgcct
    36061 ccgggataac cgaatacttg tttaacgccc tgatcgataa gcgatcggac gaccatctcg
    36121 gctccagaca acatctccat ggcctgcctc actgtttgac ggaaaaatgt gtaaaacaca
    36181 ttaaccgccc gatcttgctt aagcaattgg cagacagaga cataaacacg aggtaataag
    36241 aataaacagg aggaaaggtg aaaagaaacg ataaaatcct ccattacaga ggattaaatt
    36301 gataataaat cggctgaatc ctacaactta cagcaatttc tctatttttt taattaatcc
    36361 taaaatagag agaacacaca ttcagaataa aattctacat tcaataaaaa atgcagaata
    36421 aaccagacat tgatgtctga cctattctgc attcagttag cgtttgcaaa ttgagactaa
    36481 ttgctcttcc atccactgat ggcctttatc gcgcccggca gcttcatgcc aggagagata
    36541 acaggttctg ctgttttgtt ttaacggcag cggtaatacc tgtaactcta aggattcagc
    36601 gaaatcttca gccaaccaac gcggcgcaat agcgaccaga tgcgtttgcg acaccacgct
    36661 cagtacactc atcattgcca tgccctgata cgcgatactg gcttgtttat ctaccgtgtc
    36721 ataccacggt tgactaaatg acgcgaaacg atcgagcgaa actgccgcat gttgttcgtt
    36781 ataaacatca tgtttcagta acgggccttt aattgttgga tgatttttgc tggctaccag
    36841 caccatttca tctttaaata atggcacact ggtaaattca ggacgatgga agtcttcata
    36901 actaatcaca aactccgttt cctgataacg cagttgatgt tcagtgttct gatttaatga
    36961 agacttgaac ataacatgta tatttggcgc agtttgctca atgtgattat aaatctgcga
    37021 ggtcagaatg ctgtctaacg gactgcaaac acaaagatga aatacacgtt cactgctcgc
    37081 gggttcaaaa cctgaacctg gcaattcatt ttgtactaat tgcaatgcct gacgaactga
    37141 accaaaaagt tgaaatgcgc gagcagtcgg ttgaatgcca cggccataac gaacaaaaag
    37201 ctcgtcatta aacatcacct tcaggcgtgc aaccgcgtta ctgaccgcag gttgcgacat
    37261 ccccagaaca tgagcggcac gggtgatgtt ttgctcctgc atcacggcat cgaaaacggt
    37321 taataagttg agatcgacca tgcgtagttg tggcttgctt aactccgccg tctctggttg
    37381 atctgtttgt acctctggca tacttaactc cactgtcaca cttaactccc tttcccttat
    37441 tggaatgcag agaaataatc ctcgagaata tgatttatca tgcatataaa ataagaaaaa
    37501 ggaaaatgag aaaaattagg aaaacataaa gatacgggtt cttataaaaa cagaacccat
    37561 tgccgagtaa agcgtcattt accgcagcat aataatccat aatgtttttg ttgcgaaaac
    37621 aatctaatca taactaccgc gaatact
//
LOCUS       DBANUU010000034        37384 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000034 DBANUU010000000
VERSION     DBANUU010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.034"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            132..3883
                     /gene="yfaL"
                     /locus_tag="ACIE68_001656"
                     /pseudo
     CDS             132..3883
                     /gene="yfaL"
                     /locus_tag="ACIE68_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
     gene            complement(4011..4733)
                     /gene="ubiG"
                     /locus_tag="ACIE68_001657"
     CDS             complement(4011..4733)
                     /gene="ubiG"
                     /locus_tag="ACIE68_001657"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFX5907988.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4880..7507
                     /gene="gyrA"
                     /locus_tag="ACIE68_001658"
     CDS             4880..7507
                     /gene="gyrA"
                     /locus_tag="ACIE68_001658"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFX5907989.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7656..9344
                     /gene="yfaA"
                     /locus_tag="ACIE68_001659"
     CDS             7656..9344
                     /gene="yfaA"
                     /locus_tag="ACIE68_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFX5907990.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERAAEKTPVRQRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGIAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9341..9964
                     /gene="yfaT"
                     /locus_tag="ACIE68_001660"
     CDS             9341..9964
                     /gene="yfaT"
                     /locus_tag="ACIE68_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFX5907991.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANEALKVHDSKWLKSNGIASQYLPPEMTLKPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWILNDSNPNFIGIYRLNFLAR"
     gene            9988..14501
                     /locus_tag="ACIE68_001661"
                     /pseudo
     CDS             9988..14501
                     /locus_tag="ACIE68_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            14502..16151
                     /gene="yfaQ"
                     /locus_tag="ACIE68_001662"
     CDS             14502..16151
                     /gene="yfaQ"
                     /locus_tag="ACIE68_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HFX5907992.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMAAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKKPQWRRILQAETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16156..16932
                     /gene="yfaP"
                     /locus_tag="ACIE68_001663"
     CDS             16156..16932
                     /gene="yfaP"
                     /locus_tag="ACIE68_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFX5907993.1"
                     /translation="MRKIFLPLLLVALSPVVHSEGVQEVEIDAPLSGWHPVEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(17006..18190)
                     /gene="atoB"
                     /locus_tag="ACIE68_001664"
     CDS             complement(17006..18190)
                     /gene="atoB"
                     /locus_tag="ACIE68_001664"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFX5907994.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(18221..19543)
                     /gene="atoE"
                     /locus_tag="ACIE68_001665"
     CDS             complement(18221..19543)
                     /gene="atoE"
                     /locus_tag="ACIE68_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFX5907995.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(19540..20190)
                     /gene="atoA"
                     /locus_tag="ACIE68_001666"
     CDS             complement(19540..20190)
                     /gene="atoA"
                     /locus_tag="ACIE68_001666"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFX5907996.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(20190..20852)
                     /gene="atoD"
                     /locus_tag="ACIE68_001667"
     CDS             complement(20190..20852)
                     /gene="atoD"
                     /locus_tag="ACIE68_001667"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFX5907997.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(21048..22433)
                     /gene="atoC"
                     /locus_tag="ACIE68_001668"
     CDS             complement(21048..22433)
                     /gene="atoC"
                     /locus_tag="ACIE68_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFX5907998.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(22430..24256)
                     /gene="atoS"
                     /locus_tag="ACIE68_001669"
     CDS             complement(22430..24256)
                     /gene="atoS"
                     /locus_tag="ACIE68_001669"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFX5907999.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            24423..27272
                     /gene="rcsC"
                     /locus_tag="ACIE68_001670"
     CDS             24423..27272
                     /gene="rcsC"
                     /locus_tag="ACIE68_001670"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFX5908000.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(27472..28122)
                     /gene="rcsB"
                     /locus_tag="ACIE68_001671"
     CDS             complement(27472..28122)
                     /gene="rcsB"
                     /locus_tag="ACIE68_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFX5908001.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(28139..30811)
                     /gene="rcsD"
                     /locus_tag="ACIE68_001672"
     CDS             complement(28139..30811)
                     /gene="rcsD"
                     /locus_tag="ACIE68_001672"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFX5908002.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEQDATATGNNDNEKEGTDSVSIHFNS
                     TKIEIASALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            31550..32653
                     /gene="ompC"
                     /locus_tag="ACIE68_001673"
     CDS             31550..32653
                     /gene="ompC"
                     /locus_tag="ACIE68_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFX5908003.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            32765..33820
                     /gene="apbE"
                     /locus_tag="ACIE68_001674"
     CDS             32765..33820
                     /gene="apbE"
                     /locus_tag="ACIE68_001674"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFX5908004.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            33894..34958
                     /gene="ada"
                     /locus_tag="ACIE68_001675"
     CDS             33894..34958
                     /gene="ada"
                     /locus_tag="ACIE68_001675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFX5908005.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPEKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            34958..35608
                     /gene="alkB"
                     /locus_tag="ACIE68_001676"
     CDS             34958..35608
                     /gene="alkB"
                     /locus_tag="ACIE68_001676"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFX5908006.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            35684..37327
                     /gene="yojI"
                     /locus_tag="ACIE68_001677"
     CDS             35684..37327
                     /gene="yojI"
                     /locus_tag="ACIE68_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFX5908007.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         9139 a         9212 c        10030 g         9003 t
ORIGIN      
        1 actgatatta ttcatcaaat aataacaaaa tagccattgc accgggttga acaatttaca
       61 aaagaaagat attccatatc gtataatgcg attaaatacg ccgtcttata gaaaatgatg
      121 gttagattaa tatgcggatt atctttctac gcaaggagta tttatcttta ctcccgtcaa
      181 tgattgcatc tcttttctct gctaacggtg tcgcggcggt cactgattca tgccagggat
      241 atgatgtcaa agcgagttgt caggccagca ggcaaagcct ttcaggcatt acgcaggact
      301 ggagtatcgc tgatgggcaa tggctggttt tttcggatat gaccaataac gccagcggtg
      361 gggccgtatt tttgcaacaa ggagcggaat tttcactatt accagaaaat gaaactggaa
      421 tgactctgtt tgccaataac accgttacag gagaatataa taacggcgga gccatatttg
      481 ctaaagaaaa ctcaacgctg aatcttactg atgttatttt ttccggtaac gtcgcaggcg
      541 gctatggtgg cgcaatctat tcttctggta ctaacgatac tggtgccgtc gatttacgtg
      601 tcactaacgc catgtttcgc aataacatcg ctaatgatgg caaaggtggc gcaatttata
      661 ccattaataa tgacgtttat ttaagtgatg ttatttttga taacaaccag gcatatacat
      721 caacaagtta cagtgatggc gatggcgggg caatcgatgt taccgataat aatagcgaca
      781 gcaagcatcc ttcaggttat acgatagtaa ataacactgc ctttacaaat aacactgccg
      841 aaggttatgg cggggcgata tataccaata gcgtgacggc tccctatctt attgatattt
      901 ctgttgatga cagctacagc cagaacggag gcgtgttagt cgatgagaac aatagcgcag
      961 caggctatgg agatggtcct tcctctgcgg cgggtggctt tatgtatctc ggcttaagtg
     1021 aagttacctt tgatattgcc gacggaaaaa cgctggttat tggcaataca gagaatgacg
     1081 gagctgttga ctctattgct ggtaccgggt taatcaccaa aacaggttcc ggcgatctgg
     1141 tacttaatgc agataacaat gactttactg gtgagatgca gattgaaaac ggtgaagtta
     1201 ccctgggccg cagcaactcc ctgatgaatg tcggcgatac gcattgccag gacgatccgc
     1261 aagactgcta cggtctgacg atagggagta ttgatcagta tcagaatcag gctgagctaa
     1321 acgttggctc gacccaacaa acttttgtgc acgcattgac gggctttcag aatggcactt
     1381 taaatatcga tgctggtggc aacgttactg ttaatcaggg cagttttgct ggcatcatcg
     1441 aaggtgctgg tcagctcacc attgcgcaaa acggcagcta cgtgctggca ggggcgcagt
     1501 cgatggcgct aaccggcgat atagtcgttg atgatggtgc ggtgctttcg ctggaaggcg
     1561 acgcggcaga tcttaccgct ctccaggacg atccgcagtc gatcgtgtta aacggcggtg
     1621 tgctcgatct ctctgatttc tccacctggc agagcggcac atcatacaac gatggccttg
     1681 aagtcagtgg cagcagcgga acggttatcg gcagtcagga tgtggtagat cttgcaggtg
     1741 gcgacaattt gcatatcggc ggcgacggga aagatggcgt ctacgtggtg gtcgatgcga
     1801 gcgacgggca ggtaagtctg gcaaacaata atagttattt gggcacaaca caaatcgcct
     1861 ccggtacgct gatggtgagc gacaactcgc agcttggaga tacccactat aaccgccagg
     1921 ttatctttac cgataagcaa caagaaagcg tgatggagat tacctccgac gttgacacgc
     1981 gttcagatgc ggcaggccac ggacgtgata ttgaaatgcg cgccgacggt gaagtggcag
     2041 tgatgcgggg gtagacacgc agtggggcgc actgatggct gacagcagcg ggcagcatca
     2101 ggatgagggt agcacattga ctaaaacggg ggctggtaca ctggagctga ccgccagcgg
     2161 tacaacgcag tcggcggtac gtgtcgaaga aggcaccctg aaaggtgatg ttgctgatat
     2221 ccttcattat gcttcgtcac tgtgggttgg tgatggggca acgttcgtta ctggcgcgga
     2281 tcaggatatt cagtcaattg atgctatttc cagcggcact atcgacatca gcgatggtac
     2341 ggttttgcgc ctgaccgggc aggatacttc cgtcgccctt aatgcctcac tatttaacgg
     2401 cgatgggacg ctggtgaatg ccaccgatgg tgtgacgttg acaggtgagc ttaataccaa
     2461 ccttgaaact gacagcctga cttatctttc caacgtgacg gttaatggca atctgaccaa
     2521 tacgtccggt gcggttagcc tgcaaaatgg cgtcgctggc gatacgctga cggtaaacgg
     2581 tgattatacc ggcggcggta cgctactgct cgatagcgaa ttaaacggcg atgactcggt
     2641 aagcgatcaa ttggtgatga acggtaatac tgctggcaac acaactgtgg tggttaactc
     2701 cattacaggg attggtgagc cgacatcgac aggcattaaa gtggttgatt tcgcagctga
     2761 tcccacgcag tttcaaaaca atgcgcagtt cagtctggca ggcagcggct acgtcaatat
     2821 gggagcgtat gactacacgc tggtggaaga taacaacgac tggtatctgc gatcgcaaga
     2881 agtaacgccg ccatcgccac ctgatccaga cccgactccc gatcctgatc ccacgccgga
     2941 tcctgatcca acacccgacc cggaacctac gcctgcttac cagccggtgt tgaatgccaa
     3001 agttggcggt tatctcaata acctgcgggc ggcaaatcag gcgtttatga tggagcgacg
     3061 cgatcacgca ggtggcgatg gtcagacgct gaatttacgt gttatcggcg gagattatca
     3121 ttacacagca gcggggcaac tggctcaaca tgaagacact tctacggtgc aacttagcgg
     3181 cgatctgttt agcgggcgct ggggcacgga tggcgagtgg atgcttggga ttgttggtgg
     3241 ctacagcgat aaccagggcg acagccgctc gaatatgacc ggaactcgcg ccgataacca
     3301 gaaccacggt tatgccgttg ggctgacatc aagctggttt cagcacggta atcagaagca
     3361 aggggcctgg ctggatagct ggctgcaata cgcgtggttt agcaatgatg tttccgaaca
     3421 agaagatggc acagatcatt accactcgtc ggggattatc gcctcgctgg aggcggggta
     3481 tcagtggtta ccggggcgtg gtgtggtgat tgaaccgcag gcgcaggtga tttatcaggg
     3541 cgtgcagcag gatgatttta ccgccgctaa ccgtgcgcgc gtgtcacaat cgcagggtga
     3601 tgatattcag acgcggctgg gtttacacag cgaatggcgt accgctgttc atgtcatacc
     3661 aacattagat ctgaattatt atcacgatcc ccattcgacg gaaattgaag aggatggcag
     3721 cactatcagt gacgatgcgg tgaagcaacg gggtgaaata aaagtgggag tcacgggcaa
     3781 tatcagtcag cgagtgtccc tgcgcggcag cgtggcgtgg cagaaaggga gtgatgattt
     3841 tgcccagacg gcagggtttt tgtcgatgac ggtgaaatgg taatcactgt ctagtcggca
     3901 cggttgccgg atgcggcgta aacgccttat ccggcctacg gggagcattt gtaggcctga
     3961 taagacgcgg caagcgtcgc atcaggcatt ggcgcggcca aacatcaacc tcacttattc
     4021 tgcgtgtgca gcatatagtt cacatccacg ccggggccga gtttaaaagt attagtgatc
     4081 gggttgtaat gcagcccagt gatatgccgc tcttttaaac tggtctgatc cacccagccc
     4141 agcaattctg ccggtttaat aaacttcttc acatcatgcg tgcctttggg caccatgcgc
     4201 aaaatatatt ccgcaccaac caccgccatc agccatgact tgccgttgcg gttaagtgtc
     4261 gagaaaaaga catcgccgcc tggtttcacc agttgcgcac aggctctgac cactgactgc
     4321 ggatcgggga cgtgctccag catctccatg caggtcacca catcatactg cccggcatgt
     4381 tttgccgcgt gctcttccac ggtttcctgc acataatcca cctgaatgcc gctttccagc
     4441 gcgtgcagtt ttgccacctg caatggctca aagcccatat ccagaccggt caccgtcgcg
     4501 ccttcgcgcg ccatactctc ggccagaatg ccgccgccac aaccgacatc gagcaccttt
     4561 ttgccaaata aaccgccagc acgctcggca atatagccca gacgcagcgg gttaatgcgg
     4621 tgcaacggtt tgaactcacc ttcaagatcc caccagcggg aggcgacggc ttcaaattta
     4681 gcgatctctt cgtggtctac gttatggttt tccggcgatt tttcggcatt cattggcact
     4741 tctactccgt aattggcaag acaaacgagt atatcaggca ttggatgtga ataaagcgta
     4801 taggtttacc tcaaactgcg cggctgtgtt ataatttgcg acctttgaat ccgggataca
     4861 gtagagggat agcggttaga tgagcgacct tgcgagagaa attacaccgg tcaacattga
     4921 ggaagagctg aagagctcct atctggatta tgcgatgtcg gtcattgttg gccgtgcgct
     4981 gccagatgtc cgagatggcc tgaagccggt acaccgtcgc gtactttacg ccatgaacgt
     5041 actaggcaat gactggaaca aagcctataa aaaatctgcc cgtgtcgttg gtgacgtaat
     5101 cggtaaatac catccccatg gtgacttggc ggtttatgac acgatcgtcc gtatggcgca
     5161 gccattctcg ctgcgttaca tgctggtaga cggtcagggt aacttcggtt ccatcgacgg
     5221 cgactctgcg gcggcaatgc gttatacgga aatccgtctg gcgaaaattg cccatgaact
     5281 gatggccgat ctcgaaaaag agacggtcga tttcgttgat aactatgacg gcacggaaaa
     5341 aattcccgac gtcatgccaa ccaaaattcc taacctgctg gtgaacggtt cttccggtat
     5401 cgccgtaggt atggcaacca acatcccgcc gcacaacctg acggaagtca tcaacggttg
     5461 tctggcgtat atcgatgatg aagacatcag cattgaaggg ctgatggaac acatcccggg
     5521 gccggacttc ccgacggcgg caatcattaa cggtcgtcgc ggtattgaag aagcttaccg
     5581 taccggtcgc ggcaaggtgt atatccgcgc tcgcgcagaa gtggaagttg acgccaaaac
     5641 cggtcgtgaa accattatcg tccacgaaat tccgtatcag gtaaacaaag cgcgcctgat
     5701 cgagaagatt gcggaactgg taaaagaaaa acgcgtggaa ggcatcagcg cgctgcgtga
     5761 cgagtctgac aaagacggta tgcgcatcgt gattgaagtg aaacgcgatg cggtcggtga
     5821 agttgtgctc aacaacctct actcccagac ccagttgcag gtttctttcg gtatcaacat
     5881 ggtggcattg caccatggtc agccgaagat catgaacctg aaagacatca tcgcggcgtt
     5941 tgttcgtcac cgccgtgaag tggtgacccg tcgtactatt ttcgaactgc gtaaagctcg
     6001 cgatcgtgct catatccttg aagcattagc cgtggcgctg gcgaacatcg acccgatcat
     6061 cgaactgatc cgtcatgcgc cgacgcctgc agaagcgaaa actgcgctgg ttgctaatcc
     6121 gtggcagctg ggcaacgttg ccgcgatgct cgaacgtgct ggcgacgatg ctgcgcgtcc
     6181 ggaatggctg gagccagagt tcggcgtgcg tgatggtctg tactacctga ccgaacagca
     6241 agctcaggcg attctggatc tgcgtttgca gaaactgacc ggccttgagc acgaaaaact
     6301 gctcgacgaa tacaaagagc tgctggatca gatcgcggaa ctgttgcgta ttcttggtag
     6361 cgccgatcgt ttgatggaag tgatccgtga agagctggag ctggttcgtg aacagttcgg
     6421 tgacaaacgt cgtactgaaa tcaccgccaa cagcgcagac atcaacctgg aagatctgat
     6481 cactcaggaa gatgtggtag tgacgctctc tcaccagggc tacgttaagt atcagccgct
     6541 ttctgaatac gaagcgcagc gtcgtggcgg gaaaggtaaa tctgccgcac gtattaaaga
     6601 agaagacttt atcgaccgac tgctggtggc gaacactcac gaccatattc tgtgcttctc
     6661 cagccgtggt cgcgtctatt cgatgaaagt ttatcagttg ccggaagcca ctcgtggcgc
     6721 gcgcggtcgt ccgatcgtca acctgctgcc gctggagcag gacgaacgta tcactgcgat
     6781 cctgccagtg accgagtttg aagaaggcgt gaaagtcttc atggcgaccg ctaacggtac
     6841 cgtgaagaaa actgtcctca ccgagttcaa ccgtctgcgt accgccggta aagtggcgat
     6901 caaactggtt gacggcgatg agctgatcgg cgttgacctg accagcggcg aagacgaagt
     6961 aatgctgttc tccgctgaag gtaaagtggt gcgctttaaa gagtcttctg tccgtgcgat
     7021 gggctgcaac accaccggtg ttcgcggtat tcgcttaggt gaaggcgata aagtcgtctc
     7081 tctgatcgtg cctcgtggcg atggcgcaat cctcaccgca acgcaaaacg gttacggtaa
     7141 acgtaccgca gtggcggaat acccaaccaa gtcgcgtgcg acgaaagggg ttatctccat
     7201 caaggttacc gaacgtaacg gtttagttgt tggcgcggta caggtagatg actgcgacca
     7261 gatcatgatg atcaccgatg ccggtacgct ggtacgtact cgcgtttcgg aaatcagcat
     7321 cgtgggccgt aacacccagg gcgtgatcct catccgtact gcggaagatg aaaacgtagt
     7381 gggtctgcaa cgtgttgctg aaccggttga cgaggaagat ctggatacca tcgacggcag
     7441 tgccgcggaa ggggacgatg aaatcgctcc ggaagtggac gttgacgacg agccagaaga
     7501 agaataattt tacttcttca tgccaaaagg gagctatctc ccttgtttga attgaaaagt
     7561 ccaggctgca aagtctgggc ttttgtcgta ttagggcacg gtaaagtttg gctgtgcccg
     7621 taaaaaatgg ctggctatac acaaggaatg tggcaatgag tggtgaaaaa aaggcgaaag
     7681 gctggcggtt ctatggtctt gtaggttttg gcgcaatagc actgctttcc gctggcgtct
     7741 gggcgttgca atatgctggc agtgggccag aaaaaacgtt gtcgccgctg gtggtgcaca
     7801 acaatctgca aatcgatctc aatgagccgg acctctttct cgacagcgac tctctgagcc
     7861 agcttcccaa agatctcctc accattccgt ttctccacga tgttctgagc gaagatttcg
     7921 ttttctatta tcagaatcat gccgatcgtc tgggcattga aggcagcatt cgtcgtattg
     7981 tctatgaaca cgatctcacg ctgaaagata agctcttttc gtcactctta gatcagcccg
     8041 cgcaggcagc gctgtggcac gataaacaag gccatctttc acattatatg gtgctgatcc
     8101 agcgcagtgg tttaagcaaa ctgctggagc cattgttgtt tgccgctacc agcgacagcc
     8161 agttaagcaa aacggaaatc agtagcatca agataaatag tgaaactatt cctgtttatc
     8221 agttgcgcta taacggcaat aacgccctga tgttcgcgac atatcaggac aagatgctgg
     8281 tgttttccag cacggatatg ctgtttaaag atgatcagca ggataccgaa gccacggcga
     8341 tcgcaagtga tttgttgagc ggcaaaaaac gctggcaagc cagctttggc ctggaagagc
     8401 gtgctgccga aaaaacgcca gtacgccagc gcatcgtggc cagcgccagg ttgctgggtt
     8461 ttggctacca gcgattaatg ccttcttttg ctggcgtacg cttcgaaatg ggtaacgacg
     8521 gctggcacag ttttgtggcg ttaaatgatg aatccgccag cgtagatgcc agtttcgatt
     8581 ttacgccggt ctggaacagt atgcctgccg gggccagctt ctgtgtggcg gtgccgtatt
     8641 cacacggtat tgccgaagag atgctttcgc acatcagcca ggaaaacgac aagctgaatg
     8701 gggcgttaga cggtgccgcg gggctgtgct ggtatgaaga ctcaaaattg caaaccccgc
     8761 tgtttgtcgg tcagtttgat ggcactgccg aacaggcgca attgccaggg aaactgttta
     8821 cgcaaaatat tggtgcgcac gaaagcaaag cgccagaagg tgttttgccg gtaagccaga
     8881 ctcagcaggg cgaagcgcaa atctggcgtc gcgaagtgag ttcccgatac ggccagtatc
     8941 cgaaagcgca ggcggcgcaa cccgatcaat taatgtcgga ttattttttc cgtgtgtcgc
     9001 tggcgatgca aaacaaaacg ctgcttttct ccctcgatga cacgctggtt aataacgcgc
     9061 tgcaaacact gaataaaacc cgcccggcaa tggtggatgt aatacccact gatggcatcg
     9121 ttccgctcta tatcaatcca caaggaatag cgaaactgct gcgtaacgaa acgttgacca
     9181 gtctgccgaa gaatctcgaa ccggtttttt ataacgccgc acaaacttta ttaatgccga
     9241 agctggacgc tttatctcaa caaccgcgtt atgtcatgaa gctggcccag atggaacccg
     9301 gtgccgcctg gcagtggcta cccataacct ggcagccgct atgaggcacg ggctgctggc
     9361 gctgatttgc tggctgtgtt gtgttgttgc ccatagcgaa atgctgaatg tcgaacaatc
     9421 cgggctgttt cgcgcctggt ttgtacgcat tgcacaagaa cagctccgcc aggggccaag
     9481 tccacgctgg tatcagcagg attgtgcggg cctggtgcga tttgcagcga acgaggcgct
     9541 gaaagttcac gacagcaaat ggctaaaaag taacggcata gcaagtcagt atttgccgcc
     9601 tgaaatgacg ttaaaacctg aacagcgtca actggcgcaa aactggaatc aggggaacgg
     9661 gaaaaccggt ccctatgtga ccgcgattaa tttgattcag tacaacagcc agtttattgg
     9721 ccaggacata aaccaggcgc tgcctggcga tatgattttt ttcgatcagg gcgatgccca
     9781 gcacttaatg gtctggatgg ggcgttacgt catctaccac accggaagcg ccacgaaaac
     9841 tgacaacgga atgcgcgcag tcagtctgca acaacttatg acatggaagg acacccgatg
     9901 gatactcaac gattccaatc ccaatttcat tggcatttat cgtttaaatt ttctggcgcg
     9961 atagcagcgt gcttgtccct gtctcttgtg ggaacagggc ttgctaatgc tgatgattcg
    10021 cttccttcca gtaactatgc gccgcccgca gggggaacat tctttttgct tgctgacagc
    10081 agttttagca gcagtgaaga ggcgaaagtg cgactggaag cgccggggcg tgattatcgg
    10141 cgctatcaga tggaagagta cggcggcgtg gacgttcgcc tgtatcgtat tcctgacccg
    10201 atggcatttt tgcgccagca gaaaaacctg catcgcattg tggtgcaacc gcaatatttg
    10261 ggcgacgggc tgaacaatac gctaacctgg ctgtgggata actggtacgg caaatctcgc
    10321 cgcgtgatgc agcgtacttt ctcttctcag tcaaggcaga atgtgactca ggcgttaccc
    10381 gaattacagc tcggcaatgc cattattaaa ccttcccgtt atgtacagaa caaccagttt
    10441 tccccgctga aaaaatatcc cctggtggaa cagttccgtt atccactatg gcaggctaaa
    10501 ccgttcgagc cgcagcaagg ggtaaaactg gaaggcgcat ccagcaattt catctcgccg
    10561 cagccgggta acatttatat tcctctcggc caacaagagc cgggactgta cctcgtcgag
    10621 gcgatggttg gtgggtatcg ggcgacgacg gtggtgtttg tttccgatac cgtggcgctt
    10681 agcaaagtgt caggcaaaga gcttctggtg tggaccgcgg gtaaaaaaca gggtgaagcg
    10741 aagcccggct cagagatctt gtggactgac ggtcttggcg tgatgacccg cggtgtgacc
    10801 gatgacagcg gtaccttgca gttacaacat atatcgccag aacgttcata cattctgggt
    10861 aaggatgctg aaggcggcgt ttttgtctcc gagaacttct tctacgaaag cgaaatctac
    10921 aacatccgct tgtatatttt taccgatcgc ccgctatatc gcgcaggcga tcgtgtcgat
    10981 gttaaagtga tcggccgcga gttccacgat ccgttgcatt catcccccat cgtcagcgcc
    11041 ccggcgaagc tttcggtgct ggacgccaac ggcagtctgt tgcaaaccgt caatgtcacg
    11101 ctggatgcgc gcaatggcgg gcagggaagt ttccgcctgc cagaaaatgc cgtagccgga
    11161 ggttatgagt tacgtcttgc ttaccgcaat caggtctata gcagcagttt tcgcgtggca
    11221 aactacatca agccacattt cgagattggt ttagctctcg acaaaaaaga gttcaaaact
    11281 ggcgaagcgg tcagcggcaa actgcaactg ctctacccgg atggcgagcc ggtaaaaaat
    11341 gcccgcgtgc agttaagttt gcgcgctcag caattatcaa tggtcggtaa cgatttgcgt
    11401 tatgccggac gtttccccgt gtcgctggaa ggcagcgaaa cggtgtccga cgccagtggt
    11461 catgtgacgt taaatctccc cgccgccgat aaaccgagcc gctatttgtt aaccgtctcc
    11521 gccagtgacg gcgcggcgta tcgcgtcacc accaccaaag agatcctcat tgaacgcggc
    11581 ctggcgcatt actctttaag taccgccgca caatacagta atagcggcga gtcggttgtg
    11641 ttccgttatg ccgcgctgga atcttcaaaa caggttcctg ttacgtatga atggttgcgt
    11701 ctcgaagacc gcacgagcca tagcggagag ctaccgtcag gcggcaaatc ctttaccgtc
    11761 aatttcgcta aacctggcaa ctacaatctg acattacgcg ataaagacgg cttaattctc
    11821 gctgggttaa gtcatgccgt cagcggtaag ggcagcacgg cgcatactgg tacggtagat
    11881 atcgtggcgg ataaaacgct gtaccagcca ggcgaaaccg cgaagatgct gattaccttt
    11941 ccggagccaa ttgatgaagc attattgacg ctggaacgcg atcgcgtgga acagcagtcg
    12001 ctgctttcgc atccggcaaa ctggctaacg ctacaacgtt taaacgatac ccagtatgaa
    12061 gcccgggttc gagtgagcaa ttcctttgcg cctaacatca ctttttcggt gctgtatacc
    12121 cgtaatggtc agtacagttt tcagaacgcc gggatcaaag ttgccgttcc tcagcttgat
    12181 atccgggtga aaacggacaa aacccattac cagcctggtg aactggtcaa tgtcgaatta
    12241 acctcgtcgc tgaaaggtaa acctgtttct gcgcagctaa cggtaggcgt ggtcgatgaa
    12301 atgatctacg cgctgcaacc agaaatcgcg ccgaatatcg gcaaattttt ctatccgctg
    12361 gggcgtaaca atgtgcgtac cagctccagt ctgtcgttta tcagctacga ccaggcgctc
    12421 tccagcgagc cggttgcgcc tggcgcgact aaccgcagcg agcggcgagt aaaaatgctt
    12481 gaacgtccac ggcgtgaaga ggtggatacc gcggcatgga tgccgtcact cacaaccgat
    12541 aaacaaggca aagcgtattt cacgttcctg atgcctgatt cgttaacccg ctggcgtatc
    12601 accgcgcgtg ggatgaacgg cgacgggctg gtcgggcagg ggcgtgctta tctgcgttcg
    12661 gaaaaaaatc tctacatgaa gtggagtatg ccaacggtgt atcgcgtggg cgacaaaccg
    12721 gcggcaggac tgtttatctt cagtcagcag gataacgaac cggtggcgct ggtgactaaa
    12781 tttgcaggcg ctgagatgcg ccagacgctg acgctgcaca aaggggcgaa ttatatttcg
    12841 ctgacgcaga atattcagca atctggcttg ttaagtgcag aactgcaaca aaatgggcaa
    12901 gtgcaggaca gcattagcac aaaactgtct tttgtggata acagctggcc cgttgaacag
    12961 cagaaaaatg tcatgctcgg tggtggcgat aacgcgctga tgttgcccga gcaggcgagc
    13021 aatatccggc tacaaagtag tgaaacgccg caggagattt tccgcaacaa tcttgatgcg
    13081 ttagtcgatg aaccgtgggg tggcgtaatc aacaccggta gccgtctgat cccgctcagt
    13141 ctcgcctggc gttcgcttgc cgatcatcaa agtgccgccg ctaacgacat tcgtcagatg
    13201 attcaggata accgtctgcg gctgatgcaa ctggcggggc ccggagcgcg ctttacctgg
    13261 tggggtgaag atggcaatgg tgacgccttc cttacggcat gggcatggta cgccgactgg
    13321 caggccagcc aggcgatcgg cgtaacgcaa caaccggaat actggcagca tatgctcgac
    13381 agttatgccg agcaggcaga taacgtgccg ttattgcatc gggcgctggt gctggcgtgg
    13441 gcacaggaga tgaatctgcc gtgcaaaacg ttgttgaaag ggttggatga agctatcgcc
    13501 cggcgcggaa ctaaaactga agatttctct gaggaagacc ccagcgatat caatgacagc
    13561 ctgatcttcg atacaccgga atctccactg gcagatgcgg tggcaaacgt cttaaccatg
    13621 acgttgctga aaaaagcgca gttgaagtcc acggtgatgc cacaggttca gcaatatgcg
    13681 agggataaag cggcaaacag caatcagccg ctggcgcaca cggttgtgct gcttaatagc
    13741 ggtggcgacg ctacccagac ggccgctatt ttaagtggtt tgaccgctga gcaatccact
    13801 attgaacgcg cgctggccat gaactggctg gcgaaatata tggcgacaat gcctccggtt
    13861 gtgttgcctg cgcctgcggg cgcatgggcc aaacataagt taactggagg gggcgaatac
    13921 tggcgttggg ttggtcaggg tgtgccggac attctctctt ttggtgacga attatcgccg
    13981 caaaatgtgc aggtccgctg gcgtgaaccg gcaaaaacgg ctcaacaaag taacattccg
    14041 gtaaccgttg aacgcagttg tatcggctta tccctggtga agaagagatg agctttactc
    14101 tgcaaccggt gaccagcaat gagattgaca gcgatgcgct gtatctcgat gaaatcacgc
    14161 ttaccagcga gcaggatgca gttctgcgct acggtcaggt ggaagtaccg ctcccaccgg
    14221 gagccgacgt tgagcgcaca acatggggca tttcggtcaa taaacccaac gccgcgaaac
    14281 aacaggggca attgctggaa aaagcacgta atgaaatggg cgaactggcc tatatggtgc
    14341 cggtgaaaga actgacggga acggtcactt tccgccattt gctgcgcttc tcgcaaaaag
    14401 ggcaattcgt tctgcctcct gctcgttatg tgcgttccta tgcacctgca cagcaaagtg
    14461 ttgcggcagg gagtgaatgg accgggatgc aggtgaaata agtgaactgg cgcagaatcg
    14521 tctggctatt ggcgttggtt actctgccaa cgcttgcgga ggagacgccg ttacaactgg
    14581 cgctgcgggg cgcgcagcac gatcaactgt atcagttgtc gtcatcaggc gtgaccaaag
    14641 tctcggcatt acctgacacg ctcaccacgc cgttagggag tttgtggaaa ctgtatgttt
    14701 acgcctggct ggaggatacc catcaaccgg agcagcccta ccagtgtcgc ggaaactccc
    14761 cggaagaggt ttattgttgt caggctgggg agagcataac gcgcgatacc gcgctggtac
    14821 gttcctgtgg gctctatttt gcgccgcagc ggttgcatat tggcgcagat gtgtgggggc
    14881 aatactggca acagcgtcag gcacctgcgt ggttagcgtc tcttacaacg ctgaagccag
    14941 aaacctcggt aacggttaaa tcgctgctcg attcattagc gacgttacct gcgcaaaaca
    15001 aagctcagga agtcttactg gatgtggtgc tggacgaggc gaaaatcggt gtcgcatcca
    15061 tgctcggtag tcgggtacgg gtgaaaacct ggagctggtt tgccgatgat aaacaggaaa
    15121 tccgccaggg tggttttgct ggctggttaa ccgatggtac accgctgtgg gttactggca
    15181 gtggcaccag caaaacggta ctaacccgtt atgcgacggt gcttaatcgg gttttgccag
    15241 tgccgacgca ggttgccagt gggcaatgtg tagaggtgga gctgtttgcc cgctatccgc
    15301 tgaagaaaat cacagcggaa aagagtacga cggcagtaaa acccggcgta cttaatgggc
    15361 gctatcgtgt gacgtttact aatggtaacc acattacgtt tgtcagccac ggtgagacga
    15421 ccttactgag cgaaaagggc aaactgaaat tgcagtcaca tctcgatcgt gaagagtacg
    15481 ttgccagagt tctggatcgc gaggcgaaaa gtacgccccc tgaggcagcg aaagccatga
    15541 ccgtggcgat tcgcactttt ttacagcaaa acgccaatcg ggaaggcgat tgcctgacca
    15601 tccctgacag cagcgccacg cagcgtgttt cggcttcgcc agctacgacg ggggcgcgaa
    15661 cgatggccgc ctggacgcag gatctcatct atgcgggcga tccggttcat tatcatggca
    15721 gtcgcgctac cgaagggacg ctttcctggc ggcaagccac ggcccaggcg gggcagggag
    15781 agcgttacga ccagatactc gcgtttgctt atcccgacaa cagccttagc cgctggggag
    15841 cgccacgctc aacctgccag ttattaccca aagcgaaagc ctggctggcg aaaaaaaagc
    15901 cgcagtggcg gcgtatatta caagctgaga cggggtacaa cgaaccagac gtgtttgcgg
    15961 tttgccgttt agtctcaggt ttcccctata ccgatcggca gcagaaacgg ctgtttattc
    16021 gtaacttctt tacgcttcag gatcggctgg atttaaccca tgagtatctg cacctggcct
    16081 ttgatggtta tcccaccggg cttgatgaga actatatcga aacgctgacc cgacaattat
    16141 taatggacta atgctatgcg aaagattttt cttccgttgt tactggtggc actttcgcct
    16201 gtcgttcaca gtgaaggtgt gcaggaagtc gagattgacg caccgctctc tggctggcat
    16261 ccggtagagg gcgaagatgc cagtttttca caatcgatca attacccggc gtcgtccgtc
    16321 aacatggcag atgatcaaaa tatctcagcg cagatccgcg gcaaaataaa gaattatgct
    16381 gcggcgggta aagttcagca gggccggctg gtggtcaacg gtgccagtat gccgcagaga
    16441 attgaatccg atggttcatt tgcacgccct tatattttca ctgaaggcag caacagcgtg
    16501 caggtcatca gcccggatgg gcaaagccga caaaaaatgc agttttactc aacgccgggt
    16561 acaggaacga ttcgtgcacg tttacggctg gttctctcgt gggatacgga caataccgat
    16621 ctcgatctcc atgttgtcac gcccgatggc gaacacgcct ggtacggtaa cacggtgctg
    16681 aaaaacagtg gtgcactgga tatggatgtc acgacggggt acggacccga gattttcgcc
    16741 atgccagcgc cagttcacgg acgctatcag gtgtatatca actactatgg cggacgcagc
    16801 gaaacggaat taaccaccgc ccaactgacg ctgatcaccg atgaaggatc ggtcaatgag
    16861 aaacaggaaa catttattgt accgatgcgt aatgctggcg agctgacgct ggtgaaaagt
    16921 tttgactggt gattaatggg gttaccttcg ctgtcgatgt tcaagaaaac gcccgataac
    16981 tttcgctatc gggcgttttt gttcgttaat tcaaccgttc aatcaccatc gcaattccct
    17041 ggccgccacc aatgcacagt gttgccagcc ccagcgtttt atcgcgtgcc tgcatggcat
    17101 gtaatagtgt gaccagaata cgagcaccac tggcaccgat aggatgcccg agcgcgatgg
    17161 ccccgccgtt gacattcact ttctcaggat caaagcccaa ggttttccca acggcaagga
    17221 actgtgcagc aaatgcttca ttagcttcga tgagatcaat gtctgccagt tgcagccccg
    17281 ccagttgtaa cgctttttgc gtggcaggta ctggccccat acccatcaat gcgggaggca
    17341 cgccaccgct ggcataactt ttaatgcgag ccaggggggt aaggcctgct gccagcgccg
    17401 cagattcttc cataatcacc agagcggcag caccgtcgtt aataccagac gcgttcccag
    17461 cggtgactgt tcctgcttta tcgaaggccg ggcgcaatgc acctaacgct tcagccgttg
    17521 aattcgcttt cgggaattcg tcttgactga agacgaaggt tttcttccga gtgacaacat
    17581 ttaccgggac gatttcggct gtaaaagcac cggactcaat tgcggctgcc gctttacgct
    17641 gtgaatgtag cgccagttca tcctgcattt cacgggtaat tccgtactct ttagccacgt
    17701 tttcggcggt aatccccata tgataaccat gggtggcgca catcaggcca tcgcgcagga
    17761 ttacgtcata aacctgtccg tctccaagac gataaccaga gcgtgctttt gcatcgagta
    17821 agtagggcgc taaactcata ttttccatac cccccgccac aatgctctgc gcctgacctg
    17881 cctgaattgc ctgggcggca agcgccacac ttttaagacc cgaaccacac actttattga
    17941 ccgtgaatcc gcacaccgtt tctgccagcc cgctttttaa cagtgcctga cgtgccggat
    18001 tttgccccaa cccggcttgc aacacgttac ccataatcac ttcatcaatg tgtagtgaat
    18061 cgatttttgc acgttcaatg gcggctttaa ttactgtcgc ccccaggtcg atggcgctgg
    18121 tggaagcgag tgaaccgtta aaactaccga tagcagtacg taccgcactg acgatgacac
    18181 aatttttcat tttatattcc ttctgtctgt agggttgccg tcagaacagc gttaaaccaa
    18241 tgacgaaaat gacgccggag aagagcaggg cagtgatgca atagcccatg atgtcgcgga
    18301 caccgagtcc ggcgattgcc agtgctggca gcgcccagaa tggttgtgcc atgttcatcc
    18361 attgctcgcc gtaggcgatc gccattaccg atttaccgag atcagcgccc agcgcctggg
    18421 ctgcgggtat cacgaaaggt ccctgaataa cccagtgacc gccgccagac ggaacggcga
    18481 agttaatcag tgcagaacta aaaaaggtca ttaccgggaa ggtgtctttg ttcgcaacat
    18541 tgatgaagaa ttcggtaatg agtccaccca gaccagaatg ctccatcatc agttggatcc
    18601 cagcgtagaa ggggaattgc accagaatac cggcagtact gcgtgctgcc gcgctgatag
    18661 cacgcatata agccattggc gttttatgta gcaacagacc cgcaatcata aacatcaggt
    18721 tgacggtatt gatggtgatg ttgaagccat gttcgctgaa gtacatcgcc aggtaagcga
    18781 taccgagtgc gccgatgatc aacgtcagaa tgcggctttc ttccagtcgc tcggatggtg
    18841 gggcatcttt cggtagctgc ttttgaaaat cagcctcttc catgagtagt ttcggatcga
    18901 tactcaccac gtcagacggt tttggcatca tcatgcgggt gataaatggc atcaccacaa
    18961 tcaacgtcac agtgatgaaa atgttaaaac cactgaacag agtatcgccc accgggatca
    19021 gcccggcgat atgctcaacc gggttgcccg gtgttgcagc caacagaggc attgatccag
    19081 agaagccgcc accccaggtg agaaaaccaa tgtaggcgca ggcaatgagc aacggataat
    19141 cagaaccggg gactcgccgg gcgacttcac gggcaaacat tgcgccgaca accaaaccaa
    19201 atccccagtt gatgacacaa gcgactgaac cgaagaaagt gaccagcatg acgccctgta
    19261 cgggcgtctt tgcggcggag gcggcagtac gcagcaaact tttcaccgga gcagagctgg
    19321 caagggcatg accggtaacg atgataagcg ccatctgcat accaaacgcc agcaagttcc
    19381 agaaaccgtc accccacatt ttcaccatgc tgatcggcgt ttgtggtgtt aaccaaagcg
    19441 cgatcacgaa tgttagcaat gtcagcaaca tggcaaagat cagtggatcg ggaagccacc
    19501 ggctgacaaa acgcgtcata aaacgcgata tgcgaccaat cataaatcac cccgttgcgt
    19561 attcagatcg gcggcgactt caaaccgagc ttctgttttg gcacgcacgg tggctaaatc
    19621 acacccgtcg gcaatttcgg tgagccacat tttgccgtca ataaaacgaa agacagccag
    19681 ttcagtaacc agcatatgca ccgcatgttg cgcagtgagt ggcatggtgc agcggcgcaa
    19741 aatttttgct gaaccatctt tggcgcaatg ttccatggcg atgatcactt tgcgcgaccc
    19801 ggtcaccaga tccatcgcgc cacccatacc gggcaccatt ttcccaggca ctacccagtt
    19861 cgcgaggttt gcttcttcgt ctacttgcaa accgccgagc acgcaggcat caatatgacc
    19921 gccacggatt agcgcaaatg acatggcgct atcaaacatg gctgcaccgg gtaaaacacc
    19981 gcacggttgc ccgccagcgt tcaccagatc tggatgcgct gtcgtgaccg ggcctaaacc
    20041 gaggaagccg ttttccgatt gcagagtgat atgaataccc tccggtaaat aattggcgac
    20101 cattgtgggt aaaccgatcc ctaagttaac gatgtcacca tcacgaagct cttgcgccac
    20161 acggcgcgca atacgttgtt tcgcatccat tatttgctct cctgtgaaac gatgatgtgg
    20221 tcgataacgg caccaggggt gacaatatgg tcaggttgca gctcgccggt ttcgaccagt
    20281 tcatctggct ctaccagcgt gatatcagcc gcaagggcta tcagggggtt aaagttgcgg
    20341 gcgctaagtt gataggtcag gttgccaagt gtgtcgcaac gatgagcgcg aattagcgcc
    20401 aggtcggcgc gcagtgggcg ttcgagcagc caagttttac cgtcgagtgt cagtgtctgt
    20461 ttgccttcct ctacgacggt gccgacaccc gttggggtga gaaaaccacc aagtccagct
    20521 ccaccacagc gaatttgctc gattagcgta ccttgcggca ccagaacgac gtccatctca
    20581 ccagatatca tgcgccgacc tgtttccggg ttggtgccga tatgtgaagc aatcactttg
    20641 cggactcgac cattgacgat gagcggaccg atgccggtat caacaaacgc ggtatcattg
    20701 gctatcaatg tcaggtcgcg aacaccagat tccagtaatg cttcaaccag gcgggatgga
    20761 gtgccaatcc ccataaatcc gcccaccatg atggtcatgc cgtcacgaaa gaagccggtg
    20821 gcgtcttgta atgtcatcaa ttttgttttc attttttatc cctcttgcat acaccgttgt
    20881 gggtacagtc aggcaatagc aaggggagtg ccagaatgag attattggat ttagttattt
    20941 aactaattga atatgaaggg ataaattgga tggggattta taaggagaat gagcggctat
    21001 gcagaaaatt gcgcactgtg caaatttctg catagcaagt tttggtgtta tacatccgcc
    21061 ggatcgatac cgtattcctg gagtttatac atcaatgcac ggcgactgat gcccagcatt
    21121 aaagcagtgc gggttcggtt tccttcttgt tgttccagca cttccataat gatgcgtttt
    21181 tcgacgcgtt taatttcctc ttttaaatta cgctcaccga caggggctgt ttttacctcg
    21241 ccagcattac agactggctg acgaatctgt ggcggaagat cctcagaaaa aatgatcggg
    21301 cctgaattca tcacgacggc gcgttcaata acgttggaaa gctctcgaat atttcccggc
    21361 catgaccagg cggtaagcag tgacattgcc atcggatcga tgtcgataat atcgcgctga
    21421 ttctcactac tgaatttttg caaaaagtga ttagctaaca gggaaatatc ttcccggcga
    21481 tcgcgcagag gcggcagtat taaatgaata acgttaaggc gataaaagag atcttcacgg
    21541 aaggtacctt cttttaccat tgcctgcaag tcgcggttgg tggcagcaat gatgcggata
    21601 tcaactttta tggtctgatg gccgccaatc cgttcaaatt cccgttcctg tagaatgcgt
    21661 agtaatttgg cttgtagtac cagcggcatt tcgccaattt cgtcgaggag cagagtacct
    21721 tcgttggctc gttcaaataa tccctgacgc aaggtttgtg caccagtaaa tgcacctttt
    21781 tcatgaccaa acagttcact ttcgagcaac gattccggca gcgccgcgca gttgacttta
    21841 atgaacggcc cctttgcccg ccgcgaattg tagtgaatcg ctctggcaat caactctttc
    21901 ccggtgccgc tttcaccgct aatcaagacg ctggcctgag aaagggcaat tttggcggtg
    21961 tctttgcaga tgtccatcat cgccgggctg ttggtgagaa tgtgccccca ttgccagctg
    22021 gtgctcagtg cctggtgcag atgacggatc tcttttttca ttgactggag ttgtaaagcg
    22081 cgctgaacga ttaaattcaa ctcatcgaga tcaaacggtt taataacata gtcgaaggct
    22141 ccgcagcgta gcgcttcgac ggcggtttcc acttccgcat aggccgtcat cagaataacg
    22201 ggtgtccggg tctcatggct gcgcatctcc tttagtgcct tgatgccgtc catctctggc
    22261 atgcggatat ccatcaacac cacatcaggg tgaatatcgg caaacaggtg taatgctgtg
    22321 cgtccgttgt tcgcacaatg tgtttcgaat ccttgtagtg caaaagcggt gctcagcata
    22381 cggcgaacat tatcttcatc atccacaata aggatgcgat taatagcagt catacagtct
    22441 gatttccctg cgggttgatc ggtaaaataa gcgtgaaggt tgcgccgtag cccggcaaac
    22501 tggcgacgcg aatatcaccc tgatgggcat taatgatgcg ttgacttaac gccagaccaa
    22561 gcccggttcc tgaggctttg gtggtgaaaa agggatcgaa gatctttttt tgcagcgaga
    22621 gatcaatgcc acagccgttg tcctctatcg aaatggcctg ttgtgagtcg ctgtattgcc
    22681 aggtttgaat gcgaattttc cctcgtgcgc tgatagcctg gacggcattg atcaggatat
    22741 tcagtagtac ctgtttgagc agttcacgat cggcgttaat cgggcttaat tcattatcca
    22801 gttcgcttat gaagtcgacc cgcgcttgta cgccggcggt ttgtaccagt accagagttt
    22861 cttcaaccaa tgcattgagg ctgacttgtt gccattgact gtggcgtgga cgtgaaaatt
    22921 cgagcaattg ctgaataact ttgttaattg aatcgatttc tttgagtact acggacagat
    22981 attcctgatg tattgggtca ctggtttgtt ggcgcaagat ctgtacataa ccacgaatag
    23041 ccgttaacgg attacgtact tcatgcgcga cgccagccat cagctcaccc agtgtggcga
    23101 ggcgttctgc ttgcgccatg cggcgctggg tttctttgcg ggcagttaaa tcagagaaaa
    23161 tcaccaaagc acctatcatt tcaccgtgcg tgttatgaat acgactggta gtgacactga
    23221 gttcaatggt gcggtcacga cctggaaaac tgatctccag cgccacatgt tcggtgccat
    23281 gttccagcgt atccagtact ggactgtaga actgagtatt gtcgaacaac atggagtaag
    23341 gctgccctac cagttcatgg cgttgatagc cagtgataac ttctgctgct gggttcatgg
    23401 tggttacatc accctggcgg tcaatggcaa tgacgccatc ggcagcgttt tcaataatca
    23461 gatcgttaag tgtccgcgtt tcacgcagtg cctgggcgag gttattaaca ctctgactga
    23521 tttgccccat ttcaccgggc aattgtggta atcgagtggg aatattttgt gccagagtcg
    23581 agaggccatc ggtgatgata tcaatattgg cgctcaggcg acgggagaaa aggacaatca
    23641 atagcaggct tatcagcaaa ccggcggtga gcacaatgat aatcctcaca tccattttcc
    23701 aggcctggcg gcgaatatct tcggttaatt cattggccca gatatagccg aggatttcac
    23761 cattacgctc aatggggagc attgaattca aaatatcgcc gcgcacctgc ctgcctgaat
    23821 aaaccaaagg ggtatttgta cgcatgactt cgcgaccagg gtgatctgcg gcaatggtaa
    23881 cgccgacatt attctgatat agcgctgaag gcgcgtaggt gattatcgca tccagcattt
    23941 tgttgtaata accagcaccg atgccaggga aggcgtgagt gatattttcg gtaatggggg
    24001 caagttctgc atttaatgcg cggatacgct cctcacgtgg taagtcgatg tagagatcat
    24061 agcgatcgcc tagtgcctga ttaagcaggt tgaccacggc agaaagtttt ttctcttttt
    24121 cagataagac tgctgaacgt ccttccgttt ctacgatata accaatagta agcgttggga
    24181 caatgaccat caggattgcc atcaggatca tttgattgcg taagcggcgt ggataaatcc
    24241 acttcatata atgcatgctg aaatccacta gtcttgtccg gtatatgacg attatcagag
    24301 gttaaggtga tgatttctcg gcggtgtatc atattccaga gaagagagaa cattgcggta
    24361 acacgctttt accgctacct taaccacact ccatcggtca cctgaggcgg agcttcgccc
    24421 ctttgaaata ccttgcttct tttcgtacaa ccctgaaagc ctcgcgctac atgttcagag
    24481 cattggcgtt agtgctctgg ctgttgattg ctttttcatc cgttttttac atcgttaatg
    24541 cgttacatca gcgagaatcg gaaattcgtc aggaatttaa tctgagttcc gatcaggctc
    24601 agcgctttat tcaacgcacc tctgatgtga tgaaagagct gaagtacatc gccgaaaatc
    24661 gcttatcggc agaaaacggt gtgctttccc cgcgtggacg agaaacgcag gcggatgtgc
    24721 ctgcgtttga accgctgttt gccgactccg attgttccgc aatgagtaac acctggcgag
    24781 gttctctgga gtcattggcg tggtttatgc gctactggcg cgataatttt tctgcggctt
    24841 acgatctaaa ccgggtattt ttaatcggca gcgataacct ctgcatggcc aatttcggtc
    24901 tgcgtgatat gccagtggaa cgcgataccg cgttgaaagc tttgcatgaa cgcatcaata
    24961 aatatcgaaa tgcaccacaa gatgatagcg gcagtaacct ctactggatc agcgaaggtc
    25021 cgcgccctgg cgtcgggtat ttttacgcgt tgacgccagt ttatctggcg aaccggttgc
    25081 aggcgctttt gggtgtcgag cagaccatcc ggatggagaa ctttttctta ccgggtacgt
    25141 tgccgatggg ggttaccatt cttgatgaaa atggtcatac cctgatttcg cttaccggac
    25201 cagaaagtaa aattaagggc gatcctcgct ggatgcagga acgctcctgg tttggctata
    25261 cggaagggtt ccgggagctg gtgctgaaga aaaatctgcc accctcatcg ctaagcatcg
    25321 tgtattcggt gccggttgat aaggtgctgg aacgcattcg catgttgatc cttaacgcaa
    25381 ttttgctgaa tgtgcttgcc ggagctgcat tgtttactct cgcacggatg tacgagcgac
    25441 gtattttcat tccggcggaa agcgacgccc tgcgactgga agaacatgag cagttcaatc
    25501 gcaagattgt cgcctccgcg ccagtgggta tctgcatttt gcgtaccgct gatggcgtca
    25561 atattttaag taacgaactg gcgcatacct atctcaatat gcttacgcat gaggaccgcc
    25621 aacgactgac gcaaattatc tgtgggcagc aggtcaattt tgttgatgtc ctgaccagca
    25681 acaataccaa tctgcaaatc agcttcgtcc attcgcgcta tcgtaatgaa aacgtggcca
    25741 tttgtgtgct ggtggatgtt tcttcgcgcg tgaagatgga agagtcgttg caggagatgg
    25801 cacaagcagc ggaacaggcg agccagtcaa aatcgatgtt ccttgccacc gtcagtcatg
    25861 agctgcgaac gccgctgtat ggcattatcg gtaacctgga tctgttgcaa accaaagagt
    25921 taccgaaagg cgtcgatcgg ctggtgacgg caatgaacaa ctcttccagc ctgttgttga
    25981 aaattatcag cgatattctc gatttctcga agattgaatc ggaacagttg aagatcgaac
    26041 cgcgtgagtt ttcaccgcgt gaagtgatga accacatcac cgccaactat ttaccgctgg
    26101 tggtacgcaa gcagctaggc ttgtactgct ttattgaacc ggatgtgcca gtggccttaa
    26161 atggcgaccc gatgcgttta cagcaggtca tctccaacct gttgagtaac gccataaaat
    26221 tcaccgatac cggctgtata gttttgcatg tccgcgctga tggcgattat ctctctatcc
    26281 gtgttcgcga taccggcgtg gggattccgg cgaaagaagt ggtgcgcttg tttgatccct
    26341 tcttccaggt cggaacgggc gtacagcgaa acttccaggg gaccggtctg ggtctggcga
    26401 tttgtgaaaa actgatcagc atgatggacg gcgatatctc ggtagattca gaaccgggaa
    26461 tgggcagcca gtttaccgtg cgtattccgt tgtacggcgc tcagtacccg cagaaaaaag
    26521 gcgtggaagg gttgagtggt aaacgctgct ggctggcggt ccgcaatgcg tcgctctgtc
    26581 agttcctgga aaccagtttg cagcgcagcg gcatcgtcgt tacaacatac gaagggcagg
    26641 aaccgactcc cgaagatgtg ttaattactg acgaggtagt gagtaaaaaa tggcagggta
    26701 gagcggtagt gaccttctgt cgtcgccata ttggtattcc gctggagaaa gcgccagggg
    26761 agtgggtaca cagtgtggct gccccgcatg agctaccggc attgttggcg cgtatttatt
    26821 tgatcgagat ggagagcgac gatcctgcta acgctctgcc gtcgacggac aaagcggtca
    26881 gcgataatga cgatatgatg attctggtcg tggatgatca tccgattaac cgacgtctgc
    26941 tggcagatca gttgggatcg ttgggctatc aatgtaaaac cgcgaatgat ggtgtcgatg
    27001 cgcttaatgt acttagcaag aatcatattg atatcgtgct tagcgacgtc aacatgccaa
    27061 atatggatgg ttaccgcttg acgcaacgca ttcgtcagtt gggactgacg ttgccggtaa
    27121 tcggagtaac tgctaatgcg ttggctgaag agaagcagcg gtgtctggag tccggtatgg
    27181 acagctgcct gtcgaagccg gtaacgctgg atgtgataaa acagacgctg acggtatatg
    27241 ccgagagggt caggaaatcg cgggaatcgt aggacggata aggtgtttat gccgcatctg
    27301 gcattcagtg caaatgccag atgcgacgct gacgcgtctt atctggccta ctttaatgta
    27361 atctgttgaa ataatgggaa tcgtaggccg gataaggcgt ttacgctgca tccggcaatc
    27421 ggtgcaaatg ccagataaga cactaacgcg tcttatctgg cctacagatg attagtcttt
    27481 atctgccgga cttaaggtca cagaagagag ataattcagc agggcgatat cgttctcgac
    27541 acccagcttc atcatcgcag atttcttctg gctactgatg gttttaatac tgcggttcag
    27601 ctttttagcg atctcggtca ccaggaagcc ttccgcaaac aggcgcagaa cttcactctc
    27661 ttttggcgag agacgcttgt caccgtaacc accagcactg attttttcca acaggcgaga
    27721 aacgctttcc ggggtaaatt tcttcccttt ctgcagcgcg gcgagagctt tcggcagatc
    27781 ggtcggtgca ccttgtttca gcacgatccc ttcgatatcc agatccaata ccgcactaag
    27841 aatcgccggg ttgttgttca tagtcagaac aatgatcgac aggcttggga aatggcgctt
    27901 gatgtacttg attaaggtaa tgccatcgcc gtacttatcg ccaggcatgg agagatcggt
    27961 aatcaacaca tgcgcatcca gtttcggcag gttgttgatc agtgctgtag agtcttcaaa
    28021 ttcgccgaca acattcaccc actcaatttg ctcaagtgat ttgcgaatac cgaacaagac
    28081 tatcggatgg tcatcggcaa taattacgtt catattgttc atgtaatagg ctaccttgct
    28141 acagcaagct cttgacataa ctgtcaatgt cgctgatgta tttttctatt cctggaacat
    28201 ccttctcacg aatcagatgt tccagcgttt cacataactg cttgccgggt accagattta
    28261 gcatggcaaa tacgccttta agacgatgag ccgtttgggc taacgcagca aagtcactgg
    28321 ttgctgcttc agtatacagc ctcttaacat catccggtac tgtgtctaca aagagcgcat
    28381 aatagccgct ggcatggagt tgcgcatttt catctccgcc cagaggcgat tctgtcacct
    28441 cttcctgcgc cagttgcact tcaattaatt gtaagaccgc ttcctgcata gcgttgctca
    28501 tattgaagtt gacgcacaat tgaccaggcc caatttcccg tacgccagac tcatcatcgc
    28561 ttaaaagcaa gccagaggca gtaagattag acggattatc cgttaaaaag atatcataat
    28621 cttgactaat taatctttca tcgggtgtga tacaggttgc accccaattt tctaactggc
    28681 gagtgacaat attccgaatt tctgccgaag taacatccac cattacgcag acatcatcca
    28741 gtaaacgctc ttcttcctct tcaacttccg ggtcagcggc gagcattttg atatgcacag
    28801 agtagcgtgt accaagccca tcccgcgttt tgatgtttaa atgaccgccc agtttacgtg
    28861 ccagttgatc gctcagccag aatgccagcg ggtccgcctt tccataacga tcgttttggg
    28921 tctggttgat aaacgggaag tgcaaattat ccatttcatg aatacttacg ccttctcccg
    28981 tgtccagaat gcgaaacgtc aggcggtctt cggaggactc atcctgatca acctcaaggg
    29041 tgatttttcc caattgcgtt gaggtcacgg catattgcat cagtagcagc aaaatacgtc
    29101 gtaaggcatc gcgatcgcca cggcgcatat cgtgtgcttt cagatgattg ttaatcagca
    29161 gttgcagacc tttacgcttg atggcaggca acactgaagg cacaacttca tcaattaaat
    29221 cctgcacgga gaacagcacc gtctcacttt tccagctatc gtccgcaagc atgttcgcta
    29281 actgtatttc atcgaccagc cgcaccagca catctgcctg attcgccagt tgtttgcttt
    29341 ccggggcgtt gagtttagcc gcgctctccg ccagggactg tgcaggttct ttcagcgcat
    29401 cgccaatgtt tttcataaag gtcatccggc cctgctggtt tttctcatac agacgctgcg
    29461 cctgcttgag tttcttgttt accagcactt cgcgatcctg atcgcgaatg atgaaaattt
    29521 gtgtgcgcgg cgcgacctgg ctgcggaaca tgcggatctc atacagctcg ttattgatcg
    29581 tcgcctgaat aatcccctga tgctgttccg ccatggtggt gatgttttgc agattcaaat
    29641 gcggcagcaa atgatcggca attttgttac ttatgacagt gcggttcgat tcctgatcgt
    29701 gaaccagcag gccgagcggc agcagtgaga ctatctcttc attgattgcc cgtaaaatgc
    29761 gcaattcgtt attgaccgcc gtgttgggta gactttctgt actacgactg gagaaatggc
    29821 ggaatgtggt atagccaaat aacgccagcg ccagcaaacc gatgttcagc agcagtggca
    29881 gcagaatgtt ttgcaacgta tccagcaata aggtgccata aggaacctgc cagaccaggc
    29941 gcatatcggt agagttgagt gccgaggcga tttcaatctt cgtactgtta aagtggatac
    30001 tgacgctatc cgtcccttct ttctcattat cattgtttcc cgttgccgtc gcgtcttgct
    30061 caaggcggaa actgtccagc ggcatacccg gtgggatcaa atcattaatc ggcagatcaa
    30121 aagccacgac cgttgccaga tgtcccggct ggttgaacgt ggtacgcaag gtaaagtaat
    30181 gaccgttctg ccaggccagg cggcgcatgt tagaaaaact ttcgcgttca tcgagggcgt
    30241 tggcctgttg caacatttct gcacgacgtg aatcaacaat gtcactgacg gtcgattctt
    30301 taaatccgga ggtgagatct ttgaggggca gggttgagat cagcaccaga ctgttatcct
    30361 gaccattcag gtaatacatt gaccacggta cattttctgc gccccacaat gtgtccagat
    30421 aggtggacat ccgttgagtc atctcaagcg ttgagttgtc gtgagagcca aagattaacg
    30481 cttccgtttt gcggcgcggt ttttccagat agtagacatc ctgtttcagg cgcgtctctt
    30541 gtaaaccttc gccggaggag ggggaggtcg tcgcggcaat attgtcgtag atctgccagg
    30601 tcacgtaacg ccaggtatcg acgcgctttt ggatagcgtg ggtaatgtcg acaatctggt
    30661 aacttttatc tttcagccag gcgttaacgg cgctttgtac cattacaccc atcgtcacca
    30721 gtaacacaat gatcaacagt aaaaagaagc gggtaatgct ccccggtagg agtgaaaagc
    30781 gggtcgtggc cgttgtctct ttctgacgca ttcgtgttta tgacctgtta aaacttcgcg
    30841 aagtgatagg gcagtataaa gggtacagtg tgaaattcca gactcttacc tgattgatga
    30901 ttctctattc tcccgtgata tttatcatcc tgcattgggg gaaatagatg tacaagcgcc
    30961 attttgctgt acaaataacc tacaaaaagc ccaacaaaaa taattgcaaa taaacaaaga
    31021 ttgctggaaa ttatgctgat gttaattatt tgtgaaatag ttaacaagcg ttatagtttt
    31081 tctgtggtag cacagaataa tgaaaagtgt gtaaagaagg gtaaaaaaaa ccggatgcga
    31141 ggcatccggt tgaaataggg gtaaacagac attcagaaat gaatgacggt aataaataaa
    31201 gttaatgatg atagcgggag ttattctagt tgcgagtgaa ggttttgttt tgacattcag
    31261 tgctgtcaaa tacttaagaa taagttattg attttaacct tgaattatta ttgcttgatg
    31321 ttaggtgctt atttcgccat tccgcaataa tcttaaaaag ttcccttaca tttacatttt
    31381 gaaacatcta tagcgataaa tgaaacatct taaaagtttt agtatcatat tcgtgttgga
    31441 ttattctgca tttttgggga gaatggactt gccgactgat taatgagggt taatcagtat
    31501 gcagtggcat aaaaaagcaa ataaaggcat ataacagagg gttaataaca tgaaagttaa
    31561 agtactgtcc ctcctggtcc cagctctgct ggtagcaggc gcagcaaacg ctgctgaagt
    31621 ttacaacaaa gacggcaaca aattagatct gtacggtaaa gtagacggcc tgcactattt
    31681 ctctgacaac aaagatgtag atggcgacca gacctacatg cgtcttggct tcaaaggtga
    31741 aactcaggtt actgaccagc tgaccggtta cggccagtgg gaatatcaga ttcagggcaa
    31801 cagcgctgaa aacgaaaaca actcctggac ccgtgtggca ttcgcaggtc tgaaattcca
    31861 ggatgtaggt tctttcgact acggtcgtaa ctacggcgtt gtttacgacg taacttcctg
    31921 gaccgacgta ctgccagaat tcggtggcga cacctacggt tctgacaact tcatgcagca
    31981 gcgtggtaac ggcttcgcga cctaccgtaa cactgacttc ttcggtctgg ttgacggcct
    32041 gaactttgct gttcagtacc agggtaaaaa cggtagcgta agcggcgaag gcatgaccaa
    32101 caacggtcgt ggtgctctgc gtcagaacgg cgacggcgtt ggcggatcta tcacttatga
    32161 ttacgaaggc ttcggtatcg gtggtgcgat ctccagctcc aaacgtactg atgatcagaa
    32221 cagcccgctg tacatcggta acggcgaccg tgctgaaacc tacaccggtg gtctgaaata
    32281 cgacgctaac aacatctacc tggctgctca gtacacccag acctataacg caactcgcgt
    32341 aggttccctg ggttgggcga acaaagcaca gaactttgaa gctgttgctc agtaccagtt
    32401 cgacttcggt ctgcgtccgt ctgtagcata cctgcagtct aaaggtaaaa acctgggtac
    32461 catcgctggt cgtaactacg acgacgaaga tatcctgaaa tatgttgatg ttggtgctac
    32521 ctactacttc aacaaaaaca tgtccaccta cgttgactac aaaatcaacc tgctggacga
    32581 caaccagttc actcgtgacg ctggcatcaa cactgataac atcgtagctc tgggtctggt
    32641 ttaccagttc taatctcgat tgatatcgaa caaagggcct gcgggccctt ttttcattgt
    32701 tttcagcgta caaacacagt tttttggtgt actcttgcga ccgttcgcat gaggataatc
    32761 acgtatggaa ataagcttta cccgcgtggc actgctggct gccgcgctct tttttgttgg
    32821 ttgcgatcaa aaaccacaac ccgccaaaac ccacgctact gaagttaccg ttcttgaagg
    32881 caaaaccatg ggtaccttct ggcgtgccag catcccgggc attgatgcca aacgcagtgc
    32941 cgaacttaaa gaaaagattc agacccagct ggacgccgac gatcagctgc tttcgaccta
    33001 taaaaaagat tccgcgctga tgcgctttaa cgattcgcaa agtttatcgc cgtggccggt
    33061 aagtgaagcg atggccgata tcgtcaccac ctcgctgcgc attggcgcga agaccgatgg
    33121 cgcgatggat ataaccgtcg ggccgctggt gaatctgtgg ggctttggcc cggaacaaca
    33181 gccggttcaa attccgagcc aggaacagat cgatgcgatg aaagccaaaa ccggcttaca
    33241 gcacctgacg gtcattaatc agtcgcatca gcaatatctg caaaaagacc tgccggattt
    33301 aaatgtcgat ctctctaccg tcggcgaagg ttatgcggcg gatcacttgg cacgcttgat
    33361 ggagcaggaa gggatttccc gctatctggt gtcggtgggc ggcgcgctga acagccgtgg
    33421 tatgaacggt gaaggccagc cgtggcgggt agcgattcaa aaaccaaccg ataaagaaaa
    33481 cgcggttcag gctgtggtgg atatcaacgg tcatgggatt agcacctctg gcagctaccg
    33541 taactattac gaactggacg gcaaacgtct ttcccatgtt atcgatccgc aaaccgggcg
    33601 tcccatcgaa cacaatctgg tatccgtgac ggtgattgct ccgacggcgc tggaagccga
    33661 tgcctgggat actggcttga tggtactcgg gccggagaaa gccaaagagg ttgttcgccg
    33721 ggaagggctg gcggtttata tgatcaccaa agaaggcgat agctttaaaa cctggatgtc
    33781 accacagttt aaaagcttcc ttgtcagcga aaaaaattaa agcgcaagat tgttggtttt
    33841 tgcgtgatgg tgaccgggca gactaaaggc tatccttaac cagggagctg attatgaaaa
    33901 aagccacatg cttaactgac gatcaacgct ggcaatctgt cttagcccgc gacccgaatg
    33961 ccgacggcga attcgttttc gccgtgcgta ccacaggcat cttttgccgt ccgtcttgcc
    34021 gcgccagaca tgctttgcgg gaaaacgtct ccttctacgc aaatgccagc gaggcactcg
    34081 ccgctggctt tcgcccctgc aaacgttgtc agccagaaaa agccaatgcc cagcaacatc
    34141 ggttggataa aatcacccac gcgtgtcgac tgctggaaca ggaaacgcct gtaacgttgg
    34201 aagccttagc cgaccaggtg gcgatgagtc catttcatct acatcggttg tttaaagcga
    34261 ctaccggaat gacgcctaaa gcctggcaac aggcctggcg cgctcgccgt ttgcgcgaat
    34321 cgctggcgaa aggggagagc gtgacgacgt ctattcttaa cgccggattc cccgacagca
    34381 gcagttacta tcgcaaagct gacgaaacgc tgggcatgac ggctaaacaa ttccgtcacg
    34441 gtggcgaaaa tctggcggtg cgttacgcgc tggctgattg tgagctgggt cgttgcctgg
    34501 tggcagaaag cgagcggggg atttgcgcga tattgctggg cgatgatgac gcgacactaa
    34561 tcagcgagtt gcagcagatg tttcccgctg ccgacaacgc gcctgccgat ctgatgtttc
    34621 agcaacatgt gcgtgaagtg atcgccagcc tcaatcaacg cgatacgccg ctgacgttac
    34681 cgctggacat tcgcggcact gcttttcagc aacaagtctg gcaggcactg cgcacgatac
    34741 cttgcggtga aaccgtcagt tatcagcaac tggctaacgc catcggcaaa ccgaaagcgg
    34801 tacgggccgt tgccagcgcc tgtgccgcca acaagctggc tatcataata ccctgtcatc
    34861 gggtggtccg tggtgatggc acactttccg gttaccgctg gggcgtgtcg cgtaaagcgc
    34921 aactgctgcg ccgcgaagct gaaaatgagg agaggtaatg ttggatctgt ttgccgatgc
    34981 tgaaccgtgg caagagccac tggcggctgg agcggtaatt ttgcaccgtt ttgcttttaa
    35041 cgctgcggag caactaatcc gcgatattaa tgacgttgcc agccagtcgc cgtttcgcca
    35101 gatggtcacc cccgggggat ataccatgtc ggtggcgatg accaactgtg ggcatctggg
    35161 ctggacgagc catcggcaag gttatctcta ttcgcccatt gatccgcaaa caaataaacc
    35221 gtggcccgcc atgccacaga gttttcataa tttatgtcaa cgtgcggcta cggcggcggg
    35281 ctatccagat ttccagccag atgcttgtct tatcaaccgc tacgctcctg gcgcgaaact
    35341 gtcgctgcat caggataaag acgaaccgga tctgcgcgcg ccaattgttt ctgtttctct
    35401 gggcttaccc gcgatttttc aatttggtgg cctgaaacgt aatgatccgc tcaaacgttt
    35461 gttgttggaa catggcgatg tggtggtatg gggcggtgaa tcgcggctgt tttatcacgg
    35521 tattcaaccg ttgaaagcgg ggtttcatcc actcaccacc gactgccgct acaacctgac
    35581 attccgtcag gcaggtaaaa aagaataaaa ataagaatta ttattgctgt gcgcgcgaag
    35641 atgtttaaac tgcgggctgt aattcattgt ccgggttttc tgcatggaac ttcttgtact
    35701 tgtctggcgg cagtatcgct ggccatttat cagtgtgatg gcgctaagcc tcgccagtgc
    35761 ggcattaggc attggcttaa ttgcttttat caatcagcgc cttatcgaaa cggcggatac
    35821 cagtctgctg gtgttgccgg agtttcttgg attattactg ctgttgatgg cagtcactct
    35881 cggatcgcaa ctggcgctca ccactttggg gcatcacttc gtttaccgac tgcgtagtga
    35941 atttatcaag cggattctgg atacccacgt cgagcgcatt gaacaactcg gtagcgcctc
    36001 gttgctggcg ggattaacca gcgatgtgcg caatatcacc attgcttttg tgcgtcttcc
    36061 ggaactggtg caggggatca ttctcactat cggttccgcg gcgtatctgt ggatgctgtc
    36121 gggcaaaatg ttgctggtaa cggctatctg gatggcgatc accatctggg gtggttttgt
    36181 actggtggcg cgggtgtaca aacatatggc gaccctgcgt gaaaccgaag acaagctgta
    36241 cacggatttt caaaccgtac tcgaagggcg caaagagctg actctgaacc gggaacgcgc
    36301 cgagtatgtg tttaacaacc tctacatccc cgatgcgcaa gagtatcgcc atcatattat
    36361 tcgcgcagac accttccatc ttagcgccgt gaactggtca aacatcatga tgctgggcgc
    36421 tatcggcctg gtgttctgga tggcgaacag tctcggttgg gctgatacca acgttgctgc
    36481 gacctattcg ttgacgctgt tattcctgcg tacgccgctg ctttcggcgg ttggcgcatt
    36541 gccgacgctg ctgacggcgc aggtggcgtt taacaagctg aacaaattcg cgctcgcgcc
    36601 tttcaaagct gagtttccgc gcccgcaggc ttttcccaac tggcaaacgc tggagctgcg
    36661 taacgtgacg tttagctatc aggataacgc gttttccgtt gggccgatta acctcaccat
    36721 taaacgtggc gagctgctgt ttctgattgg tggcaacggt agtggaaaat cgacgctggc
    36781 gatgttgctg acgggcttgt accagccaca aagcggcgaa atcttgctgg atggcaaacc
    36841 tgtcagtggc gaacaaccgg aagattatcg caaactgttt tcggcagtgt ttaccgatgt
    36901 ctggctgttt gatcaactgc tggggccgga gggtaaaccc gctaacccgc aactggttga
    36961 gaagtggctg gcgcagctga aaatggctca taagcttgag ttaagcaacg ggcgtattgt
    37021 taacctgaag ttatcaaaag ggcagaaaaa acgcgtggcg ctgttgctgg cgctggcaga
    37081 agaacgcgat attatcctgc tggatgaatg ggcggcggat caggatccac acttccgtcg
    37141 tgagttttat caggtgttgc tgccgctgat gcaggagatg ggtaaaacta ttttcgctat
    37201 cagtcatgat gatcattact ttatccacgc cgaccgcctg ctggaaatgc gcaatgggca
    37261 acttagcgag ctgacgggcg aagagcgcga tgccgcttcg cgtgatgccg ttgcccggac
    37321 ggcataatat tacgacgctg acctggcgga ctgctgccgt caggtcaata tgctaaaaat
    37381 ccct
//
LOCUS       DBANUU010000035        37339 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000035 DBANUU010000000
VERSION     DBANUU010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.035"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            286..1602
                     /gene="fucP"
                     /locus_tag="ACIE68_001678"
     CDS             286..1602
                     /gene="fucP"
                     /locus_tag="ACIE68_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFX5908008.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            1635..3410
                     /gene="fucI"
                     /locus_tag="ACIE68_001679"
     CDS             1635..3410
                     /gene="fucI"
                     /locus_tag="ACIE68_001679"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFX5908009.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            3519..4937
                     /gene="fucK"
                     /locus_tag="ACIE68_001680"
     CDS             3519..4937
                     /gene="fucK"
                     /locus_tag="ACIE68_001680"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFX5908010.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            4939..5361
                     /gene="fucU"
                     /locus_tag="ACIE68_001681"
     CDS             4939..5361
                     /gene="fucU"
                     /locus_tag="ACIE68_001681"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFX5908011.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            5419..6150
                     /gene="fucR"
                     /locus_tag="ACIE68_001682"
     CDS             5419..6150
                     /gene="fucR"
                     /locus_tag="ACIE68_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFX5908012.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(6194..7294)
                     /gene="rlmM"
                     /locus_tag="ACIE68_001683"
     CDS             complement(6194..7294)
                     /gene="rlmM"
                     /locus_tag="ACIE68_001683"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFX5908013.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7287..7682)
                     /gene="ygdD"
                     /locus_tag="ACIE68_001684"
     CDS             complement(7287..7682)
                     /gene="ygdD"
                     /locus_tag="ACIE68_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFX5908014.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(7701..8618)
                     /gene="gcvA"
                     /locus_tag="ACIE68_001685"
     CDS             complement(7701..8618)
                     /gene="gcvA"
                     /locus_tag="ACIE68_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFX5908015.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(8969..9196)
                     /gene="ygdI"
                     /locus_tag="ACIE68_001686"
     CDS             complement(8969..9196)
                     /gene="ygdI"
                     /locus_tag="ACIE68_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFX5908016.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            9388..10593
                     /gene="csdA"
                     /locus_tag="ACIE68_001687"
     CDS             9388..10593
                     /gene="csdA"
                     /locus_tag="ACIE68_001687"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFX5908017.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            10593..11036
                     /gene="csdE"
                     /locus_tag="ACIE68_001688"
     CDS             10593..11036
                     /gene="csdE"
                     /locus_tag="ACIE68_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFX5908018.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(11087..11893)
                     /gene="tcdA"
                     /locus_tag="ACIE68_001689"
     CDS             complement(11087..11893)
                     /gene="tcdA"
                     /locus_tag="ACIE68_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFX5908019.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAKVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(12132..13229)
                     /gene="mltA"
                     /locus_tag="ACIE68_001690"
     CDS             complement(12132..13229)
                     /gene="mltA"
                     /locus_tag="ACIE68_001690"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFX5908020.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            13438..13514
                     /locus_tag="ACIE68_001691"
     tRNA            13438..13514
                     /locus_tag="ACIE68_001691"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13472..13474,aa:Met,seq:cat)
     gene            complement(13589..14842)
                     /gene="amiC"
                     /locus_tag="ACIE68_001692"
     CDS             complement(13589..14842)
                     /gene="amiC"
                     /locus_tag="ACIE68_001692"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFX5908021.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            15074..16405
                     /gene="argA"
                     /locus_tag="ACIE68_001693"
     CDS             15074..16405
                     /gene="argA"
                     /locus_tag="ACIE68_001693"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFX5908022.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERITAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(16467..18293)
                     /gene="recD"
                     /locus_tag="ACIE68_001694"
     CDS             complement(16467..18293)
                     /gene="recD"
                     /locus_tag="ACIE68_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFX5908023.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(18293..21835)
                     /gene="recB"
                     /locus_tag="ACIE68_001695"
     CDS             complement(18293..21835)
                     /gene="recB"
                     /locus_tag="ACIE68_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFX5908024.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIEQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDVVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(21828..24716)
                     /gene="ptrA"
                     /locus_tag="ACIE68_001696"
     CDS             complement(21828..24716)
                     /gene="ptrA"
                     /locus_tag="ACIE68_001696"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFX5908025.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(24892..28260)
                     /gene="recC"
                     /locus_tag="ACIE68_001697"
     CDS             complement(24892..28260)
                     /gene="recC"
                     /locus_tag="ACIE68_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFX5908026.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKSHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(28273..28596)
                     /gene="ppdC"
                     /locus_tag="ACIE68_001698"
     CDS             complement(28273..28596)
                     /gene="ppdC"
                     /locus_tag="ACIE68_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFX5908027.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(28581..28988)
                     /gene="ygdB"
                     /locus_tag="ACIE68_001699"
     CDS             complement(28581..28988)
                     /gene="ygdB"
                     /locus_tag="ACIE68_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFX5908028.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(28985..29548)
                     /gene="ppdB"
                     /locus_tag="ACIE68_001700"
     CDS             complement(28985..29548)
                     /gene="ppdB"
                     /locus_tag="ACIE68_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFX5908029.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(29539..30009)
                     /gene="ppdA"
                     /locus_tag="ACIE68_001701"
     CDS             complement(29539..30009)
                     /gene="ppdA"
                     /locus_tag="ACIE68_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFX5908030.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(30194..30988)
                     /gene="thyA"
                     /locus_tag="ACIE68_001702"
     CDS             complement(30194..30988)
                     /gene="thyA"
                     /locus_tag="ACIE68_001702"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFX5908031.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(30995..31870)
                     /gene="lgt"
                     /locus_tag="ACIE68_001703"
     CDS             complement(30995..31870)
                     /gene="lgt"
                     /locus_tag="ACIE68_001703"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFX5908032.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(32021..34267)
                     /gene="ptsP"
                     /locus_tag="ACIE68_001704"
     CDS             complement(32021..34267)
                     /gene="ptsP"
                     /locus_tag="ACIE68_001704"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFX5908033.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYADAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(34280..34810)
                     /gene="rppH"
                     /locus_tag="ACIE68_001705"
     CDS             complement(34280..34810)
                     /gene="rppH"
                     /locus_tag="ACIE68_001705"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFX5908034.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(35163..35309)
                     /gene="ygdT"
                     /locus_tag="ACIE68_001706"
     CDS             complement(35163..35309)
                     /gene="ygdT"
                     /locus_tag="ACIE68_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFX5908035.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            35495..36184
                     /gene="mutH"
                     /locus_tag="ACIE68_001707"
     CDS             35495..36184
                     /gene="mutH"
                     /locus_tag="ACIE68_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFX5908036.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            36253..36966
                     /gene="ygdQ"
                     /locus_tag="ACIE68_001708"
     CDS             36253..36966
                     /gene="ygdQ"
                     /locus_tag="ACIE68_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFX5908037.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     CAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            37104..37322
                     /gene="ygdR"
                     /locus_tag="ACIE68_001709"
     CDS             37104..37322
                     /gene="ygdR"
                     /locus_tag="ACIE68_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFX5908038.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT         9021 a        10073 c         9311 g         8934 t
ORIGIN      
        1 gcataaacaa ttaatacggt catctggttt gtgtttttta tgatttattt tctgaaacgg
       61 gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc cagctaataa
      121 gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca tgagttcatt
      181 tcagacaggc aaatattcac tgatatgaag cccgaactcg ctggttttgc acttttgaaa
      241 acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg aaacacatca
      301 atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag aagttacatt
      361 attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa taaccttaac
      421 gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca ggctggcctg
      481 atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg gatattgatg
      541 aaaaaactca gttataaagc agggattatt accggattat ttttatatgc cttgggcgct
      601 gcattattct ggcccgctgc agaaataatg aactacacct tatttttagt tggcctattt
      661 attattgcag ccggattagg ttgtctggaa actgccgcaa acccttttgt tacggtatta
      721 gggccagaaa gcagtggtca cttccgctta aatcttgcgc aaacatttaa ctcgtttggc
      781 gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc acatcaatcg
      841 caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca cagcctggta
      901 ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt cgccctgctg
      961 atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc caaacaagga
     1021 tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg ggcggtatta
     1081 gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat tcgctacgct
     1141 gtagaagaaa ttccaggtat gactgccggc tttgccgcta actatttaac cggaaccatg
     1201 gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt cgcaccacac
     1261 aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc agccttcgct
     1321 ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc gattcagtac
     1381 ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata cggttcgtcc
     1441 ttcatcgtta tgaccatcat tggcggcggt attgtcactc cggtcatggg ttttgtcagt
     1501 gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt cgcggtcatc
     1561 tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt tccgaataaa
     1621 gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc ccggttattg
     1681 acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat atggcgaaag
     1741 ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc gtcgagtgtg
     1801 tcatttccga tacctgtatc gcgggtatgg ctgaagccgc tgcttgcgaa gaaaaattca
     1861 gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat ggcagtgaaa
     1921 ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc actgaacgtc
     1981 ccggcgctgt ttacctggca gcggctctgg cagctcacag ccagaaaggc atcccggcat
     2041 tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct gccgatgttg
     2101 aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg aaaggtaaaa
     2161 gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttccatcgtt gatcacaact
     2221 tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa ctgcgtcgcc
     2281 gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc tgggctgata
     2341 aaaacttccg ctatggcgaa gatgaaaata acaaacagta tcaacgcaat gccgagcaaa
     2401 gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac atgatgcaag
     2461 gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac aacgccatcg
     2521 ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt gacaccgccg
     2581 aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt gtcgtggcga
     2641 ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc accggcaccg
     2701 ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag cgtgtaacgg
     2761 ggcataaact cgatggactg gcagaacacg gcatcatcca tttgatcaac tcaggttctg
     2821 ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg atgaagccgc
     2881 actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccgaa tggtgcccgg
     2941 cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc gaaggcggcg
     3001 tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta ctgcaaatcg
     3061 cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac aaacgcacca
     3121 actcaacctg gccaaccacc tggtttgcac cgcgcctcac cggtaaaggg ccgtttacgg
     3181 atgtgtactc ggtaatggcg aactggggcg ctaaccatgg ggttctgacc atcggccacg
     3241 ttggcgctga ctttatcact ctcgcctcca tgctgcgtat cccggtatgt atgcacaacg
     3301 ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg gatattgaag
     3361 gccaggatta ccgcgcttgc cagaactacg gcccgttgta caagcgttaa tacattactc
     3421 cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag tggttagccg
     3481 gataagcaat gttatccggc tatattgcag gagcgattat gaaacaagaa gttatcctgg
     3541 tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag ggaaaaattg
     3601 ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac acctggcacc
     3661 agtggtcttt agacgccatt ttgcaacgct ttgctgattg ctgtcggcaa atcaatagtg
     3721 aactgactga atgccacatc cgcggtatcg ccgtcaccac ctttggtgtg gatggcgctc
     3781 tggtagataa gcaaggcaat ctgctctatc cgatcattag ctggaaatgt ccgcgaacag
     3841 cagcggttat ggacaatatt gaacagttga tctccgcaca gcggttgcag gctatttctg
     3901 gcgtcggagc ctttagtttc aatacgttat ataagttggt gtggttgaaa gaaaatcatc
     3961 cacaactgct ggaacgcgcg cacgcctggc tctttatttc gtcgctgatt aaccaccgtt
     4021 taaccggcga attcactact gatatcacga tggccggaac cagccagatg ctggatatcc
     4081 agcaacgcga tttcagtccg caaattttac aagccaccgg tattccacgc cgactcttcc
     4141 ctcgtctggt ggaagcgggt gaacagattg gtacgctaca gaacagcgcc gcagcaatgc
     4201 tcggcttacc cgttggcata ccggtgattt ccgcaggtca cgatacccag ttcgcccttt
     4261 ttggcgctgg tgccgaccaa aatgaacccg tgctctcttc cggtacatgg gaaattttaa
     4321 tggtccgtag cgcccaggtt gatacttcgc tgttaagtca gtacgccggt tccacctgcg
     4381 aactggatag ccaggcaggg ttgtataacc caggtatgca atggctggca tccggcgtgc
     4441 tggaatgggt gagaaaactg ttctggacgg ctgaaacgcc ctggcaaatg ttgattgaag
     4501 aagctcgtct gatcgcgcct ggcgcggatg gcgtaaaaat gcagtgtgat ttattgtcgt
     4561 gtcagaacgc tggctggcaa ggagtgacgc ttaataccac gcgggggcat ttctatcgcg
     4621 cggcgctgga agggttaact gcgcaattac agcgcaatct acagatgctg gaaaaaatcg
     4681 ggcactttaa ggcctctgaa ttattgttag tcggtggagg aagtcgcaac acattgtgga
     4741 atcagattaa agccaatatg cttgatattc cggtaaaagt tctcgacgac gccgaaacga
     4801 ccgtcgcagg agctgcgctg ttcggttggt atggcgtagg ggaattcaac agcccggaag
     4861 aagcccgtgc acagattcat tatcagtacc gttatttcta cccgcaaact gaacctgaat
     4921 ttatagagga agtgtgaaat gctgaaaaca atttcgccgt taatttctcc cgaactattg
     4981 aaagtgctgg cagagatggg acatggagat gaaattattt tttccgatgc tcactttccc
     5041 gcccattcga tgggaccgca ggtgatccgc gctgatggcc tgttggtgag cgacttgctc
     5101 caggcgatta tcccgttatt tgagctggac agttatgccc cgccgctggt gatgatggca
     5161 gcggtagaag gtgacactct cgatcctgaa gtagaacgac gttaccgtaa tgcgctttca
     5221 ctacaagccc cgtgtcctga catcatccgc atcaatcgtt ttgcgtttta tgaacgggcg
     5281 caaaaagcct ttgcgatcgt tatcacaggc gaacgagcga agtacgggaa tattctttta
     5341 aaaaaagggg taacaccgta atctcatacc ggtacgcccg catgacgcgg gcggttatcg
     5401 aatgatgggg tgaaaaatat gaaagcggca cgccagcaag cgatagtcga cctgctgctg
     5461 aaccatacca gcctgaccac ggaagctctc tctgaacagc taaaggtcag taaagaaacc
     5521 attcgtcgcg atctcaatga attacagacg cagggtaaaa ttctgcgcaa tcatggacgc
     5581 gctaaatata tccaccgtca aaatcaagac agtggcgatc cctttcacat ccggctgaaa
     5641 agccattatg cgcataaagc agatatcgcg cgcgaggcgc tcgcgtggat tgaagaaggg
     5701 atggtgatag ccttagacgc cagttcaact tgctggtatc tggcacgcca gttgcctgac
     5761 atcaacattc aggtcttcac caatagccat ccgatttgcc atgaactcgg taaacgcgaa
     5821 cgcattcaac tgatcagttc cggcggcaca cttgagcgca aatatggctg ttacgtcaat
     5881 ccctcgctga tttcccaact taaatcgctg gaaatcgatc tgtttatttt ttcttgtgaa
     5941 gggatcgata gcagcggcgc actgtgggac tccaatgcca tcaacgctga ttacaaatcg
     6001 atgctattaa aacgtgccgc gcaatcgttg ttattgattg ataaaagtaa atttaatcgt
     6061 tcaggggaag cccgcatcgg gcatctggat gaggtaacgc acattatttc tgatgagcgc
     6121 caggttgcaa cttctttggt aacagcctga cggaaagggt agcaggccgg agacgacgcc
     6181 ccggccttgc ctgttatcgc tcgtcgcgac gaccacccac cgcagcccag atgcggcgga
     6241 cgtgcaccgt cacttcttcg cgatcgtgat acaactgccg cgcctgaatc tgagcattta
     6301 tgccatgttc atcaagctgt gcctgaatat acgccagatt gtgtgatact tcttcgtagc
     6361 gttttttcat cggcagtttg agattgaaaa tggtttcacg gcaccagcca ttaaccagcc
     6421 actgcgccat caatgccgca actttagccg gtttttcaac catatcgcat accatccagg
     6481 agatattgct gcgtgtcgga cggaatttga aaccgtcttc ccgcagccac gtcacctgcc
     6541 cggtatccat cagactttgc gccatcgggc cgttgtcgac ggaataaacc cacatgttgc
     6601 gcttcaccag ttggtaggtc cagccgcctg ggcaagcgcc caaatccacc gcccacatcc
     6661 cgttcgccag gcgttcatcc cactcatccg caggaataaa cacatgaaat gcctcttcca
     6721 gtttgagtgt ggaacgactc ggcgcatctg ccggaaattt caggcgcgga atgcccatat
     6781 agaacggcga attattgttg ctgtatgagt aaccggtata gcagcaacct ggtgcaatga
     6841 agaatacatg cacaaccgga cgcttcggcg tttcatagtt cgccagcacc ccggcatcgc
     6901 gcaaggcagc gcgtagcggc acggtgaatt tacggcagaa tttcagcaac tctttgcttt
     6961 cgttggtatc ggcaacttca acacgcagtt caccgccctt ctctactacg ccctgtaaca
     7021 tgccgacaat gggggtaata cgatcttctg gcggcaaatg ctgcaggagt tcccccacca
     7081 caaaccactg gcgggcaaaa attaatgaac tgaacggcag ctcacggatt aacttatcgc
     7141 catcatcagg ttgataacat tcataaatga cataacccgc attctctttc acgcgggcaa
     7201 aaccgaaaat ttcccgctgg ccggctttat cggtaatttc tgcggcgcac tctttttcaa
     7261 agcccggacg gcacagcaat acaaccttat tcatgactta cgcccttgcg ctttaaacgg
     7321 atagcaccaa ctaacattaa cgcccagccc gcaaggaaac tcacgccgcc aaccggtgtg
     7381 acaaacgccc acaaacgcag atgggacagt gccaggcaat aaaggctgcc gctgaacagc
     7441 accgtgccta acgcgaggaa aacgctactc cagtaaaacc agatactgat gcgacgctgc
     7501 attgccaccg ccagacctaa gatcgccagc gtatgaaacg cctggtattc gaggccggtc
     7561 tggatccagc ccatctcaac ggcgcccatg gttttactta acacatgcgc gccaaaagcg
     7621 cccagagcca caaaaatgaa gccgctaatg gcggcgaaaa tcagcataaa acggctggtc
     7681 atggtcgtac cctacgtaaa ttattgttca taacgaaagc ggaatttttc ttgttcagca
     7741 gcggctttcg ccaggatcca ttggcgaaag gcggctattt tacccagttc tgcctggctg
     7801 tcatgacaaa ccagataaaa agcgttttta ctgaccagaa catcattaaa cgggcaaaca
     7861 agacgtccgg cttcaatttc agattgcgcc atcacgttat ttgccagcgc cactccctgc
     7921 ccgtgaatag ccgcttgcag caccatggcg ctatggctaa aaattggccc ttgctgaacg
     7981 ttgatatgat ttaaccccaa ttgtcgggta tatgtctgcc agtcacggcg cgaagcatca
     8041 tgtaataacg tatgtttagc cagatcttcc ggggtcttca agggtttttc gccagtcagc
     8101 agtagcggcg aacacaccgg caataaatat tcggcgtaaa gtttttccac ccgtagcccc
     8161 ggccagttgc cccgaccata aaatatcgcc acatcaacat catccgccag cttatcttcc
     8221 tgacgatcaa ccgcctggat tcgaacgtca attcccggat aagctgaatt aaagctggaa
     8281 agtcgcggaa ccaaccaatg aatggcgaaa ctggggagta aactgaccgt caacgcccct
     8341 ttggcgctac gggcctggag tttacgcgtc gcttcggtta attgcgaaaa tatctctttg
     8401 atatcgagga aatagctttg accttcctcg gtcagcagga gtgaacgatt acggcggcgg
     8461 aacagtttta gccccagaaa atcctcaaga gacttgattt gatgacttac tgcggcctgg
     8521 gtcacaaaaa gctcttctgc tgcgcgagtg aaacttaaat ggcgtgctgc ggcatcaaaa
     8581 actcgtaagg catttagcgg tggtaatcgt ttagacatgg cttttaaact ttgatgttaa
     8641 atgaatttaa caattagatc acactgtgta acctattagt ttttttaatc tgagccatta
     8701 taaattgtcc gttgagcttc taccagcaaa tacctatagt ggcggcactt cctgagccgg
     8761 aacgaaaagt tttatcggaa tgcgtgttct ggtgaacttt tggcttacgg ttgtgatgtt
     8821 gtgttgttgt gtttgcaatt ggtctgcgat tcagaccatg gtagcaaagc tacctttttt
     8881 cacttcctgt acatttaccc tgtctgtcca tagtgattaa tgtagcaccg cctaattgcg
     8941 gtgctttttt ttaccttgcg atcgcgaatt actgatccag ttcgaccatc tctttcacgt
     9001 cggtacggtt gatctgctgt ttgttgccat tagcgtcttt atacgaaatc ataccggtat
     9061 cgttatcagt ctgtggtttg ccgtcagaga cgatggtacg tccgtcattg gtgtgcatca
     9121 catagttcga accggaacag gcgctcaggg caaaagtcag catacaggca gaaataattg
     9181 cggcagtctt tttcatcatc ttctccttaa agcgattaat aaatcttaat agtattgatt
     9241 aacaggctaa aattaacgcc taacactatt cagcatatgt tactgacgcg gcttcgccag
     9301 gatatccaga taattctgat ggttagcact ctccttgtat caaagtgaat tttgcgtcac
     9361 gatcggtgca tcaagccgag gagtaccatg aacgttttta atcccgcgca gtttcgcgcc
     9421 cagtttcccg cactacagga tgcgggcgtc tatctcgaca gcgccgcgac cgcgcttaaa
     9481 cctgaagccg tggttgaagc cacccgacag ttttacagtc tgagcgccgg aaacgtccat
     9541 cgcagccagt ttgccgaagc ccaacgcctg accgcgcgtt atgaagctgc gcgggaaaaa
     9601 gtggcgcaat tactgaatgc accggatgat aaaactatcg tctggacgcg cggcaccact
     9661 gaatccatca acatggtggc acaatgctat gcgcgtccgc gtctgcaacc gggcgatgag
     9721 attattgtca gcgtggcaga acaccacgct aacctcgtcc cctggctgat ggtcgcccaa
     9781 caaactggag ccaaagtggt gaaattgccg cttaatgcgc agcgactgcc ggatgtcgat
     9841 ttgttgccag aactgattac tccccgtagt cggattctgg cgttgggtca gatgtcgaac
     9901 gttactggcg gttgcccgga tctggcgcga gcgattacct ttgctcattc agccgggatg
     9961 gtggtgatgg ttgatggtgc tcagggggcg gtgcatttcc ccgcagatgt tcagcaacta
    10021 gatatcgatt tctatgcttt ctctggtcac aaactgtatg gcccgacggg tatcggcgtg
    10081 ctgtatggca aatcagaact gctggaagcg atgtcgccct ggctgggtgg cggcaaaatg
    10141 attcacgaag tgagttttga cggcttcacg actcaatctg cgccgtggaa actggaagct
    10201 ggaacgccaa atgtcgctgg tgtcatagga ttaagcgcgg cgctggaatg gctggcagat
    10261 tacgatatca accaggccga aagctggagc cgtagcttag caacgctggc ggaagatgcg
    10321 ctggcgaaac gtcccggctt tcgttcattc cgctgccagg attccagcct gctggccttt
    10381 gattttgctg gcgttcatca tagcgatatg gtgacgctgc tggcggagta cggtattgcc
    10441 ctgcgggccg ggcagcattg cgctcagccg ctactggcag aattaggcgt aaccggcaca
    10501 ctgcgcgcct cttttgcgcc atataataca aagagtgatg tggatgcgct ggtgaatgcc
    10561 gttgaccgcg cgctggaatt attggtggat taatgacaaa cccgcaattc gccggacatc
    10621 cgttcggcac aaccgtaacc gcagaaacgt tacgcaatac cttcgcaccg ttgtcgcaat
    10681 gggaagataa atatcgccag ttgatcatgc tggggaaaca gcttccggct ttgccagacg
    10741 agttaaaagc gcaggctaaa gagattgccg gatgcgaaaa ccgcgtctgg ctgggatata
    10801 ccgtggctga aaacggtaaa atgcacttct ttggcgacag cgaaggacgt atagtgcgcg
    10861 gcctgctggc ggtgctgttg actgccgttg aggggaaaac cgccgccgaa ttgcaggcac
    10921 agtcgccgct ggcattgttt gatgagctgg gattgcgtgg gcagcttagc gcctcacgca
    10981 gccaggggtt gaatgcgtta agcgaggcga ttatcgctgc gacgaagcag gtttaagcac
    11041 agaaccgggt cggataagac gttcgcgtct catccgacct gattgtttaa ccctgacgcg
    11101 ccgctttcgc catcatcttc ttcagcgcat gagaaaccgc aacaaaccca aaggtggcgg
    11161 tcaccatcgt tgccgcgcca aatccagatg cacaatccat ccgcttcggc ccttctgccg
    11221 tggctttcat cgcacacacc gtaccgtctg actgcgggta caccagcgct tcagtagaaa
    11281 acacgcaatc cacgccgagc ttacctttac tgtttttcac tacgccaaaa tcgcttttca
    11341 ggcgctcgcg caacttcgcc gccagcgggt cctgaatcgt tttcgccaga tcggtaacct
    11401 gaatctgcgt cgggtcaatc tgcccacccg cgccaccggt cgtgaccagc gggattttat
    11461 tgcgccgaca ataagcaatc agcgccgctt tgggtcgtac actatcaatg gcatcaatca
    11521 cgtacgaata accggcactc atatactgcg ctacgttatc cggcgtcacg aaatcatcca
    11581 ccaccgttac acggcactcc gggttaatct ggcgaatacg ttccgccatg actttcgctt
    11641 ttgccagccc aacgttatcg cgcagggcat gaatttgccg attggtattg gtgacgcaca
    11701 catcatccat atcgataagc gtgattgcgc caatccccgt gcgcgccagc gcttccgccg
    11761 cccaggaacc gacaccaccg ataccgacca cacaaatatg cgcgtcagca aacagttgca
    11821 acgctttttc accatacaga cgcgctgtgc caccaaaacg ctgacgccat gcatcactaa
    11881 ttaccacaga catagcacct cagaattaaa aagggtgagg ttttccctca ccctaaacgc
    11941 ataaatgcct gatgcgctac gcttatcagg cctacataac tcctgcaata ttttggattt
    12001 gcatgatttg tgggccggat aaggcgttca cgccgcatcc ggcatgaaca acgcgcactt
    12061 agtcaataat caggatgtga ggttacccct caccctgtca tatccgtaaa aacggcatac
    12121 agaatatcac atcagccgct aaagacgtta cctgcgcccg gggcggtttt cagcacccag
    12181 acacgtccat agtggttgta ccaacctgcg cggtgtccgg cttccggccc gatcccttga
    12241 tagatatcga agtgttggcc tttgattgcg cccccgacat ccaacgccac catcagacgc
    12301 aattcgtact gaccgttaaa tttgccgtta ttatccagca gcggcacttc tgctagcaag
    12361 gtagtacctg gcggaataat tgaacgatcg gaggcaacag aagcacggcc aaccagcggc
    12421 accgcacttg cccctttcac cggtgcaaaa gattgcggtt taaagaagac gaaagacggg
    12481 ttctgttcca gcagctcgcg aacctcggct tcactgtgtg tttcgcccca atgacgaatc
    12541 gcctgcatcg acatatcttc ttttttcact tcgccacggt cgatcagcac cttaccaatg
    12601 ctgcgatagg catgaccgtt tttccctgca tagctgaaaa agttaagcgg actgccatca
    12661 ccaaagtcga tatacccgct accctgcaca tccataatga agttatccat cagggagttg
    12721 ctgtaagcga gaatatattt atcactcagc gcccctgcgt agatctccgc acgagacggc
    12781 agacgaccac gttttggcgg catacggtaa atcggatact ggaactcgcc ctggcgggta
    12841 tggcgcgcct gaattaccgg cgtgtaatag cctgtaaact gcacgttacc atagttgtcg
    12901 gcaccttcca tctgccaggc atcaatgccg aactgacgca tattgcgggt atcaccgcct
    12961 gcgcgcagcc actcttgcac cgcgttataa acattactct ggttgccata cagacgcggt
    13021 gacgaattac ggatatggtt aatttgctcg gcaaaatcac cggcgttaat cggcgcgcca
    13081 acggcatctg gctggttcac cagagagaaa ggctgggtaa atttcccgtc tttatattgc
    13141 tgtccgcgat cggttggttt ggaagagcag gcggcaagca ttgccacaac cgtgcccata
    13201 agaaggtact ttacccaacg tcctttcatt gttctcttct tcaggttaaa aaataaggcg
    13261 caacgaagat aacaaaccgc cgcgtttaat gaaatgagca gcctctccct gacgcaaatt
    13321 ttgcacaaaa aataggcttt agtgatttgt ttttgttcaa aatcatgcca aatccgtgat
    13381 cggggtaaaa aaaaggttgc atgaaaacgc gagcggagta tagtgcgcat ccacggacgc
    13441 ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg gttcaaatcc
    13501 ggcccccgca accaattatt gaacacccta acgggtgttt ttttgtttct gctctcccat
    13561 aaaaaaagcg ccattcagcg cctttttatc atccccttct cgccagcgtc gccccatcgg
    13621 caaaatacgc tttaattccc gcaagaatag actccgcaac ttcctgctgg aaagtcgccg
    13681 ttttcagttt acgctcttcc tcaacgttac tgataaacgc cgtttcgacc agaatggagg
    13741 gaatatcagg cgctttcagc acggcaaacc cggcctgttc aacctgattt ttatgcagct
    13801 tgttgatttt accgagctta ttcagcaccg ctttaccaaa cttaaggctg tcggcaatgg
    13861 tcagcgactg caccatatcg aacatggtgt ggtcgacata gcggtcaccg cttttgctca
    13921 cgccaccaat caagtccgag gcgttctggg tctgtgccag atattttgcc gcagtactgg
    13981 ttgcgccttt ggttgagagc gcaaacactg aggaaccgct cggctggcga ctggtaaagg
    14041 cgtcggcatg gatagagaca aacaggtcgg cacgctgttt ctgggctttt gctacgcgca
    14101 cttgcaacgg aatgaagatg tcttcattgc gcgtcatgta caccttcata ttgccctctt
    14161 tctcgatcag agagcgcaga cggcgagcta tttgcaatac cacgtctttt tcgcgcgttt
    14221 tgtatttccc caccgcaccg gagtcttcgc cgccgtggcc agggtcaagc ataataacaa
    14281 taggacgatc gcgccctgct ttacccggtt gtggaccact ttgtgctggc ggcacctgct
    14341 tttcgaggtc gcctttgttg taatcctcca gcagcgccag cagcgggtcc tgcatatcct
    14401 gtgcattggc cggatagagg tccatcacca gacgctcttt aaacccggcg accggcgcaa
    14461 gggcaaacag ctgcggtttt acgttttgct ttaattcaaa aaccatccgt acggtctgcg
    14521 ggtcaaattg cccgacgcgc gccgacttga tgaacgggtc gtctgcacga atttgtgccg
    14581 ccatcccctt gagtaccgag ttcaggttta catcttcgat atccaccacc acgcgttccg
    14641 ggttactcaa cgcgaactgc ttatatttca gctgacgatt tgattctacc gtcacgcggg
    14701 tgtagctgga cgcaggccag acgcgcaccg ccacgacctg gctaaccgca gccaggctga
    14761 cctgacttac gctcaatagc cacatagcgc ccgcgccttg cagtaaacga cgacggctga
    14821 ttgcagtgtt ggatcctgac atgcctctcc cgagcaaaag aaatctaagc tgtgtaacaa
    14881 gtaaacgact aatttgaccg gaaactttag cgaaagacgc ataatctgtc atctaataaa
    14941 cggtaaacat tctttttata ttcacggcat tactgataaa aaagtcgctc tcgcataaaa
    15001 tttacacttg caccctgcga aaaaacagaa taaaaataca ataatttcga ataataatgc
    15061 aaaggggtgt gccgtggtaa aggaacgtaa aaccgagttg gtcgagggat tccgccattc
    15121 ggttccctat atcaataccc accggggaaa aacgtttgtc atcatgctcg gcggtgaagc
    15181 cattgagcat gagaatttct ccagtatcgt taatgatatc gggttgttgc acagcctcgg
    15241 catccgtctg gtggtggtct atggcgcacg tccgcagatc gacgcaaatc tggctgcgca
    15301 tcaccacgaa ccgctgtatc acaagaatat acgtgtgacc gacgccaaaa cactggaact
    15361 ggtgaagcag gctgcgggaa cattgcaact ggatattact gctcgcctgt cgatgagtct
    15421 caataacacg ccgctacagg gcgcgcatat caacgtcgtt agtggcaatt ttattattgc
    15481 ccagccgctg ggcgtcgatg acggcgtgga ttactgccat agcgggcgta tccggcggat
    15541 tgatgaagac gcgatccatc gtcaactgga cagcggtgca atagtgctaa tggggccggt
    15601 cgctgtttca gtcactggcg agagctttaa tctgacctcg gaagagattg ccactcaact
    15661 agccatcaaa ctgaaagctg aaaagatgat tggtttttgc tcttcccagg gcgttactaa
    15721 tgacgacggt gatattgtct cagaactttt ccctaacgaa gcgcaagcgc gggtagaagc
    15781 ccaggaagag aaaggcgatt acaactccgg tacggtgcgc tttttgcgtg gcgcagtgaa
    15841 agcctgccgc agcggcgtgc gtcgctgtca tttaatcagt tatcaggaag atggcgcgct
    15901 gttgcaagag ttgttctcac gcgacggtat cggtacgcag attgtgatgg aaagcgccga
    15961 gcagattcgt cgcgcaacaa tcaacgatat tggcggtatt ctggagttga ttcgcccgct
    16021 ggagcagcaa ggtattctgg tgcgccgttc tcgcgagcag ctggagatgg aaatcgacaa
    16081 attcaccatt attcagcgcg ataacacgac tattgcctgc gccgcgctct atccgttccc
    16141 ggaagagaag attggggaaa tggcctgtgt ggcagttcac ccggattacc gcagttcatc
    16201 acggggcgag gttctgctgg aacgcattac cgctcaggcg aagcagagcg gcttaagcaa
    16261 attgtttgtg ctgaccacgc gcagtattca ctggttccag gaacgtggat ttaccccggt
    16321 ggatattgat ttactgcccg agagcaaaaa gcagttgtac aactaccagc gtaaatccaa
    16381 agtgttgatg gcggatttag ggtaagcgat gaaaattgtc ggatgcgaca tgcgtaacac
    16441 tcgtgcgtcg catccggcaa ttacgtttat tcccgcgaac taaacaacgc cgccagacca
    16501 ctgcgccgct cagtacgagt ggcgattgcc gcacttaata tacgctcatc ggcatacagc
    16561 gacagacggc gacgcgcgcg ggtcaccgcg gtgtatacca gctctcgcgt tactaccggc
    16621 gtgcgttggc tcggtaaaat caacgccgca tggtcgaact ccgatccctg cgatttatgt
    16681 accgtcatcg cccacgtagt ttcgtgctct ggcaagcgac tcggttgcac agacttaata
    16741 ttgccgtccg gcatcgcaaa ccagacgcgc gtcccctgcc cgcgatccag cgcaataccg
    16801 atatcgccat taaacaaccc aagcgcgctg tcattacggg caatcattac cggtcgacct
    16861 tcgtaccaac gagagtgcgg atgacgatga attttgcgct tctgttgcat aaactgctca
    16921 attcgctcat tcagtccagc cacgccaaac ggcccttccc gcagggcgca caaaagttgg
    16981 tactcattga acgcctgaat gattaaatcc ggctcggcac gcgcttgcag cagatccaga
    17041 taacgtccat aacccgcaag agcttcctca agcatcgcaa tataatcttc tccgctctgt
    17101 aaaagccgtt tttcgatatc agtaaaatcc tgctgaaaaa cggttttcac tgccgtttta
    17161 tcaccacggt tgatcgccgc agctaactga ccaatgccag aatcactgcc gaaacgatag
    17221 cttttttgca gcaggcagag actgtcgcgc aaagatgccg cttctgtgcc agttcctgcc
    17281 ggaacgtgag ttcccgtcag gcggcttagc tgcccggcac gctcggcggt aaagccggca
    17341 ttggcataag cgcagatatc gcccagcaca gccccagcct caaccgaggc cagttgatca
    17401 cgatcgccga gaaagatcac tcgcgcatga tcgggcaagg cgtcgatcag tctcgacatc
    17461 ataggcagat cgatcattga cgcttcatct accaccagca catcaagatg cagcgggtta
    17521 ccggcatgat gacgtaaacg ctggctaccc ggctgcgcac ccagcaatcg gtgcaaagta
    17581 ctggcatctt ccggaatgcg tttcttttgt tcatcggtca gcggtaactg tcgcaaagcc
    17641 ttgccgagag attcggttaa gcgcgcggca gctttacccg ttggtgcagc cagacggata
    17701 cggcagcgtt cgccgtcggc catttgaatt aacgctgcca gtaacttcgc cacggtggtc
    17761 gttttaccgg tgcctgggcc gccggaaatc accgagatcc gccgcgtcag cgccactgcc
    17821 gctgcaactt tttgccagtt aatttcatcg cttactggaa aaagtttgtc cagggtttgc
    17881 gccagtagag cttcatcaac ctcaatggca tgattcacct cattgaagaa gcgcgctacc
    17941 gtccgctcgt tacaccacat gcgattcaaa taaagacgat cgccacagag gatcatcggc
    18001 gtgggttcat ctcccctgct gaccgcttga gaagccagca agcattcttc ccaattttgt
    18061 agctcaccga tttcactgac acaggtcgcc aacagcggat gcgacgcctc gttattttcc
    18121 agtcgtgaaa gcggcaaaca gacgtgtccc tctccggcat catgacttaa cagtgccgcc
    18181 gcgagggtga cggcaggatg ttcatctccc gccacggtca gggcaaattg cacatccagc
    18241 gggcgtagct gtttgtgctc cacagcttcc agtaattgct tttgcaattt cattacgcct
    18301 cctccagggt cataccggca aacatctcat ccatcagggc aatcaacccg gcgttgggtc
    18361 gggttgtgta aatcccttgt tgcggatgtt ctttatcaac gccacgcagg aacagataga
    18421 taacgccgcc aaagtgacga tcatagtcgt aatcagcaat gcgatggcgc agataacgat
    18481 gcagcgccag ggtataaagc tgatattgca gatcatagcg gtgtgcctgc atcgctgctg
    18541 ccatagcctg ttgggtgtaa gccgaactgt cttcacccag ccagttggat ttatagtcga
    18601 gcaggtaata acgcccttcg tggcggaata ccaggtcgat aaagcctttt aacatgccgc
    18661 gtacctgcat gaactccagc ggcgggcagc cagcggatag cgggtcaaac tggcgaatta
    18721 gtgcatcaag ctggctggcg atgagcggtt cgctaatcgg caggtaaaac tccatctcca
    18781 cctgtttatc gcggtcggaa agctgactca ggctaacacc cgtttcattg agaggtgcct
    18841 ggaggacagc cgtgatccac tcggtcaaca ccggttccca ctgcgattca aagccgccga
    18901 gttccagttt ttcctgtacc cagttcgggt caaccggctg ggtaaaatcg aggtcttcaa
    18961 acaaactgtg caagaatgtc cccggtgacg caccgcgcgg gaactgatgc ggtgttaacg
    19021 tcggttcttc aacgacgctg accacgcctg cggcatcaac atccagccgt ggcatcagat
    19081 cctgagcgat accgtgacca cgctgctgca agccggagta gctggtgaca cgccagttat
    19141 cgccgggcaa tcgttgtagt gtcctcgcat tcagttctgc agttaaagca tcattaacct
    19201 gccagggctg gttatcacca gtttgtgccg tttgccaggc aatatcatca tcgcataacg
    19261 cttcaataca ggtgcgaagc cctgccgcat cttgcggttc ccctttttgc agcaaacgcc
    19321 cgagcgcact ttggtggacg tcggtgtcac cttttttatc gccacgacgg cgcaccagcg
    19381 gtgcaacgcc gagactgcaa tgccaaaccg aacgcgtcag cgccacgtaa agcaaacgca
    19441 gatcttccgc cagacgttcg acctccgcga ggtcgacgct ttctggcgca gcgttaagat
    19501 ccaaaactgc ctcaaacgag tggcgatcgt gataaaacgc ctgatcctgg acgcggaaat
    19561 tggtgataaa cggcagccag accaatggat attccagccc tttcgatttg tggatcgtga
    19621 caatctgcac cagatgttta tcactttcga gacgcatttg ttggctggag gcattactgt
    19681 ctggctcgag gatatgttgc gataaccagc gtaccagcgc atgttcactt tccagctgcg
    19741 ttccggcttc ttgtagcagt tcgctgatat gcaagatatc ggtaagacgc cgctcaccgc
    19801 ctgccgttgc cagcaagttt tcagcaatgt tacgcgccga catcagcgcc cgcagcatcg
    19861 gcataacgcc acgtttgcgc cagatttgcc gataaccatc gaactcttcg actaccacat
    19921 cccacgcatg ttcgtcattg ttcagcgttt cgatatccag cgcgttcagc cccatcattg
    19981 acgttgccag cgcactacgc agggtgttct cacgttcggg cgtcatcacc gcctgcaaca
    20041 accaaagcat ttcctgcgct tccagagttt caaaaacact gtcgcggttc gaaaggtaaa
    20101 cggaagggat ttccagcaac gttaaggcgt cgcgcacctg ggcggcctcc tggcggctgc
    20161 gcaccagcac actgatgtcc gaagcacgca ccggacgcgc gtcgtcgccg ttcatcagca
    20221 acgcttcgcc ccgctgtccg gcttgtagcc agtcgcggat ttgcgcagca catacctgcg
    20281 ccatggtact ttgatagtcg ccaacgccgc agctttcgcc ttccatcagc cacattttca
    20341 tcgcaggctg cgtttcacct ttaaacacaa aacgtaacgc ctgatttttc ccggctgatt
    20401 tcactggaat aaacggtatt tcgcgaaaca tgaacgcgtc atcagtctgg ctgaaaagct
    20461 tgttcacact gttcaccatt cctggtgcgg aacgccagtt ggtgtctaaa gtgtagtggg
    20521 cgtgaacttc gctacgcgcc ttcatataag tgaagatatc cgcaccccgg aatgcatata
    20581 tggcctgttt cgggtcgcca atcagcaaca atgcggtttc cggctgatgg tgccagatac
    20641 ggcgaaaaat tcggtactgc tgggggtcgg tatcctgaaa ttcatcaatc attgccaccg
    20701 ggaatcgcgt acggatcgcc gctgccaaca cctcgccgct ttcgctacgc agcgcggaat
    20761 cgagccgact taacatgtca tcaaaaccca attcgccacg gcggcgtttt tcacgcgcta
    20821 ctgtttcgcg gatctcagcc aatgcgcggg ttatcaccag atcacggatc gacaatggtt
    20881 ctgcaagcag ttgttcgatc gcctcaaaca gtggatgtcg cggggtttcc cccccggctt
    20941 tcgtgcgatc ttctaagaaa cgctgagaga atttttccag cgactccggt aactgataac
    21001 tgttggtctc ttcttctgcc caggcgctga tcttctcgat ccatttagcc tgattgctac
    21061 ggttaaactt gcgtcgatca ataccagaag attcgatcag cgcatccagt tcacccactg
    21121 cgtcgcgcca ctgctgtttt accgtatcaa tacgcgccac aatttgcgcg tggcgggaag
    21181 ccagcgtttc atcatcgggc ggcggtgctt tgataaccgg tgcttcgcct tgcagataac
    21241 gattaatatc gcgcagcaac gcctgcggcc ctttccaggt ttcaaagacc accagggcaa
    21301 tttcacgcgg cagcgggtag cagtggcgac gccagaaatc ggcgcaggcc tggtagcgta
    21361 gcagagactc atcttcaatc agctgctgct caaacagcat accggattca aaggcattca
    21421 ggttgagcat gcgctggcaa aaaccgtgaa tagtaaagac tgccgcttca tccatctgcc
    21481 gttcggctaa caacaaccac tgcgcggctt gcgctttatc gtcgatctct tccagcaggc
    21541 gttcgtacag tggattgtcg gtggtttcac gcagacaggc gatgcgcaac tcgtggatat
    21601 tgctacggat acgaccgcgc aattctgccg tggcagcctc ggtaaaggtg accaccagca
    21661 gttcttcaac ggtcagcggg cggggaaagg cggcggaacc gcctagtcca agtaacaggc
    21721 gcaaatagag cgccgcaatc gtaaaggttt tgcctgtgcc ggcagaggct tcaatcaggc
    21781 gctcgccctg taagggcaag cgcaaaggat ctagtgtctc ggcgacatca ctcattcttt
    21841 tcactcatca ggggcattgt ttgctgcaac gcgctgacgt tctcccacac tttccagcct
    21901 tcaggatgca catattcggc tttcccgttc tggctgccgg aaatctgcga caggatagcc
    21961 atgccttgcg gctcgaccac cgcctgatgg aagaaatcag caagtttttg cggcgtcagc
    22021 agttttatct gggccacgat tttatcacgc gaatcgaagc gcatattgcc gcgatcgaaa
    22081 tctttactta acttcgatgc ttcttcgccg agcgtttgcg gtgcctgcag catctgggta
    22141 attaccgcct gctggatttg cgcaaactca tctggcttca tcgctcgcaa ttttgcctct
    22201 gcggttggga aaaacgcctt gtaacgctcc cacaagaatg aaggctgttt atcattgctt
    22261 tgcaaaagga agcccatgcc ccactgacgc cccacgctca ttggaaacgc aaacacggca
    22321 tagcccaatt gttcttcggt acgcaactga ttgtagaacc acggctgtac gatctgcccc
    22381 aacagagagc tataggctga gctggtgtat tcatcgtagc cagtcggtac aaataccgct
    22441 gccagtgcgg agtcggtgct gttaccggct ttttcaaaga tgacggattg ttttttatcg
    22501 accactacat ctttgtttcg acaccactct gaaccatcag cgcccaactg tttttgcaca
    22561 tcgcgtgcca gcgttgttgc ctgggcctcg gtcatgttgc cgataaccat aaactctggt
    22621 cgagcccctg attttaaggc gtcgcgatag gccagcacct ctttcaacgt aatggagggc
    22681 aaaattttac gccgttcatc tcgcgagaag tacggcactt gcgagagcat ctgcgcgggc
    22741 ataatcgcct gctcaaacgc tttacccttt tctgcggaat ccatcatctg gttataccag
    22801 gacttcgcct gctcaagctg atcttccgta gcggtatagc taaagtaccc ctcgagcaat
    22861 gcctggaaca gctgcggcag acgctgggtg taaccattag cattaaccat aaggccgttg
    22921 ttagcgttgg tggaaaaact tatgccacca accgacgcct ggttgcttaa ctgatcaagc
    22981 gccagccctg cgagataatc attgagcgca aacatcacct gattgcgggc gctgtccatg
    23041 gctttcggat tacgcaaaat caggctgaca tcagctttgg gttcgctgga aaaataacgg
    23101 cttggcgcat acaccacgcg cagattcgac tcatcaacaa tcagctctgg atggtcatat
    23161 ttcttctctg acttaatcag cgagaaatcg tcaggaatat aggggttaag ctccggtaag
    23221 gagagcgcaa tattggcagc tttttgctgc cagtcagcga aagtttgttc gctgatttta
    23281 tcgacctgat acggcgcatc gacaaaataa gccgttttat tgtgcggctc tttcgggctg
    23341 atataccaga tacgcgcatt ctgcggcgtc atcatcgcca gacgttcttt tactgcttta
    23401 gcatcgtacc gatcggcaat attgactgca tccagcgtat gctcaaccgg aacgcgaatc
    23461 atggtatctg ccagccattc gacgtaatcc atatcacggg tgatcgacgg ataacggaag
    23521 tcgatatcca gcacattcgc cagttcatcg aagtattgtt tatcaatgcc tttttcacgt
    23581 aacagattga gatagctaaa aattgccgcc acaacctgat cgcgattagc caggccttta
    23641 tcggttaaag acgcagagat cgctaatacg ccgctgttgc cgttgacgat aggatcggag
    23701 ttggcgctaa tgccctcaac taatccctgc ttttgcagcc agtcggaaag tgtacctggg
    23761 ctgcgattac caatcagata ggtaatcaat tcatccgttt tactacggaa cttcgctgag
    23821 ttgttatcga tgcgaaactc aacgcgcaga actttacgcg gcagtgccgg gacgtaatga
    23881 atgataatgc ccttttgcgc gtcggtgact accggcacgg tgatttccgg ttttttgctc
    23941 tctttgttcg gcacgcgacc aaaggtgtcc gccgccattt ttgccaactc cggcagcggt
    24001 ttattgctgt aaataaccgc cttcatcaga ttggcggaat agtacttctc gtggaaatct
    24061 ttcagcgcct gctgcaccgg attaccaggt ttatcgctta aagtttcgag gttaccacca
    24121 gaaaactttg aaccggggtg tgccgggtta atggtttctg cgctgacctg tgccatgcgc
    24181 atcccgtcac gcgtacgcgc catggttaat tcagcgttca ccgcattacg ctcacgttcg
    24241 gcatatttct tgtcgagcaa aggttcagca atagcatcgg ccaggcggtc taccgcacca
    24301 ggcaaggcgt cgttctcaac ttccagatag aaagccgtgc gatacggcgc agtgctggca
    24361 ttgtgactac cgccgtgcat tttgagatat tcggccagac tgtcagcctg cgggtacttt
    24421 ttcgacccca tcagactcat atgttcaagg taatgtgcca gcccctggta cgcctcggga
    24481 tcttccagcg acccaacggg caccaccagc gccgagagcg atttaactgc ctgcggatca
    24541 gaaaccagca agaccaccat accgttatcc agacgtatag cctgatactg gcggttatct
    24601 ttatcacttt tacggatggt ttcctgaatc ggctgccatc ccgtttctgc ctgacttaag
    24661 ggtgcccaaa gggcaacaaa caacaataat gctttgaacc aggtgctgcg gggcattcac
    24721 ggacctcata atcaacttta ttttctggcc agattcaaca ggttaacgca aatcatctgc
    24781 cagaatttaa tcttgtgctg cacgagtcag cctatgtcta tatatccatc agtccgtgac
    24841 tggtgcgcat cataatgtaa gcgaatgaat tgcgcaattt ttatacagca ctcatgactg
    24901 attaaagcga aacagcggta acaggaaacg ttgcgactgt tcaacgatgg cctccattgt
    24961 ctctggtgtt aattgccgcc agagccgttg ataccagata tcatcacctt cgccacgcac
    25021 catcatgttg ccttcgtaag cctgaaggaa tttcgtacgg gctttttgca acgtggaatc
    25081 gtcatccagc atggcatcgt tttgcgcgtc ataacaggtt tttagccacg cgccgccact
    25141 ttcaggtaac accagcaatg gcgcggacat tccttcacga tacccctcaa tcagttgtga
    25201 gaggtaatgc aaagcctgtt cggctgcaag cggcggaaaa cgccactcgc cgtctttgcg
    25261 tagaaaaagg cgactttcac cattaccacc gctggcacag tagacaaggt gttccagcca
    25321 aagttgcatt ccctgggcca cacttaataa agagggacgc cagcgcaaca ggccatccgg
    25381 ctgcacctgc ggcaaccagc cagttatctg cacaccgttg caggcgagat caatttccat
    25441 actctgcccc ggctggcgac aggcaatgac tctgtcggca agctgctgca tctcctggca
    25501 ctgtgtttcc cagaaaattt caccaaaagc gccatacggt aaatcccctg ccgcccggaa
    25561 gcggcggaac aagcgttcgg catcatcctg ctcaaccagt gcattcaata actgctgatt
    25621 gatttgataa cggctaagcc cttccagaat aaatggctcg gtgtcgggga tttcgctgtc
    25681 ttcggtacgg aagttcacct gcaaacgcat ctggaaaaat gcccgcaccg gatgtgccca
    25741 gaatcgttgt agcgtttcca gcggcacggt ttccggtaag gtaaacggca gcggctgaac
    25801 aaattcagaa tgtgctttac cagcctggct ggccgcaggt agccattcac gagcataact
    25861 ttgtcgttcg cctggctggt agttttgcgg atcaaacggc attcgggtat gaaggcaagt
    25921 aagatgcgat tttacccttg cctcgctttc atcacagttg agcgcctcat cgcccggtag
    25981 ataatgactt tgtccgatgt agtcgatcag ttcctgcacc agtaccgacg ggaaacgttc
    26041 actgttatcc tgaatggaac gcccgatata gctgatatag agtttttgct gcgcggaaat
    26101 taacgcttcc aggaacagat agcggtcgtc atcgcgacgg ctacggtcgc cacgcttcgg
    26161 tttctggctc atcaggtcaa agcccaatgg cgcaagctgg cgtggataaa cgccgtcgtt
    26221 cattcccagc aggcaaacca ctttgaatgg gatggaacgc attggcatca gagtacaaat
    26281 gttaaccggt ccggcgagaa aacgctgact gatacgttcc tgatccaggc gctgtgccag
    26341 ttcatcacgc aatagtgaca gcggcaccgc gtcgccatac tgcgcaccta aaccttcggc
    26401 gataatcgcc tgccattgtt gttcgatcag cgtcatcgcc gcttcggttt ccgcatccgg
    26461 caggaagaag gcgttgagca tatcgcgaca aaccggcaac cactcttcca gcggacgctc
    26521 ctgcgccagc ccgcgacgcc agatgtttag ctgcattagc agtgaagcca gatgccccac
    26581 cagttctgca attaagccgc tcgattcatc ataaggtaga accgattgcc actcgccctg
    26641 cgcgctctcc atcgcgtagc ccagcaacat acgcgtcagg ccaaaccgcc aggtgtgttg
    26701 accggtggcg gggagttcca gctcgcgaac gttatcgtca tctatccccc agcgaatgcc
    26761 ggattcgttg acccactggc gtaaataacg cagcccttct tcggtgatgt caaaccgcgc
    26821 cgccagcacc ggcacatcca gcagcgccag cacatcctct gacacaaagc gactgtcagg
    26881 cagtgataac aggctgataa acgcttccag taccggatgt gactgccgcg cccgacggtc
    26941 ggaaatggcg taaggcaggt aacgatccgc aggtgcgcta ccaaacacag cctgaataaa
    27001 cggactgtag ctgtcgatgt cagccaccat cacgatgatg tcgcgcggag taagtgtcgg
    27061 gtcttcctcc agcatcgcca gcaagcgatc gtgtaaaact tcaacttcac gctgcgggct
    27121 atggcaaacg tggaaggtga tactgctatc cagtggatca agcgggcgtt tgttatcgct
    27181 acgggaaaac tcttcgatgt tcacacccgc aacggcgcgg ttttccagtt ccagaatgtc
    27241 agactgaatg ttatgcagca ggttgtctgg cgtcacatcg acaaaagcgt ccagctcctg
    27301 gctgctctcc aggtcagaaa ggagataaat gtagtcgcgc ccaagcttcc cccatgaggc
    27361 cagcagcggg ttgccgacat cctgttcacc atcgctgtta aagagctgtc cggcattttc
    27421 gctatcgcga aatagcggca attcgcgatc ttcaaaactg tgtcggcgct ggcgagtcag
    27481 tagtttcgcc agataagcag gatctttaat gtcgccccag taataacggc aggggttggt
    27541 aaacaggaga tggatttcaa tatgtttacc cagcgcctgt agcgcctgga gataaacagg
    27601 cggtaacgcg gaaataccgc atataaagac gcgcgaaggt agccccggcg ggcaggtcgt
    27661 cgcggactcc agcgtttcga taaagcgctg atagagattg gcgcggtgcc agcgcggttg
    27721 cccgagttga tgggtatatt ccaccaacgc cttccacaac ggcgcttgcc aggcctgtgc
    27781 ttctcccagc ccttcaacca aatgtcctgt ttcccactgt gccagccagt ccggacgata
    27841 gaccagatac tggtcaaaca ggtccgccgc ttttgaagaa agctggaaca gttttcgctt
    27901 gtcgctatcg tcagtcagat aatgccgcaa cagggtaaag tcttcgcgct ccaacaattg
    27961 cggcagcaga gtcatcagtt tccagctcat gctctgtttg ttaaaggcgc tctctttggg
    28021 gatttccggt aacacccgga cgaacatatc ccagataaag ctcgctggca gcggaaaatc
    28081 aatgtttgcc gcaataccaa acttttgcga cagggtcatt tgcagccact gcgccatacc
    28141 ggtactttgc accagaatca tctctggttc gaaaggatcg tccagccgtt cgcgttcgac
    28201 aataaactcc atcaacgctt ccagcacgtc cagacgattg gaatggtaga cccttaacat
    28261 agcggctcct gactactgac gattcgggca atgcaggcgg gtcatctcgc cttctctgcc
    28321 cccgggtgaa actagcgtaa cgctgatgct gacacatccc gcctgcgatg tctgcattcg
    28381 gttgacctgc cagttggcag gtggcgaaat cgcgcgcagt tgcgtttgct gccaaccatg
    28441 ccgccagagc tgttggtact ggtttctgct ggcgagactg ttcattaatg ttcgctggat
    28501 acccgataac gcagtgacaa tcatcaccat cagcaccatc gcaaccatta cctccggcag
    28561 gctaaagcct tgttgattct tcagggaagc tgacataacg ccctctcttt cagcggacaa
    28621 aaatcgctcc agccgcgtgg cgaaaaaaca atgtttccat caatgacttc gcctgttcgc
    28681 cacaacgaaa cgccttcata accggcaatc aataaggctt cattatctgc cagtaaacgc
    28741 aaacaaacct gggcatcggt tccagcgtac tgcgagcact gaactgctgg ctgcgtctgc
    28801 caggagtgca tttttcccca cgccagcgcc gactgaacga tggcctggcg gcgcaatgac
    28861 tgactttcca tgctcacgcg agaggcaaaa ctgcgatcct gctgactcat tccttgcaat
    28921 agcaagctac ccaaaaccag cagcatcagg accagagcca gtgacgaaac acctttttcg
    28981 cggttcacag gttgaatcct gtcacgctat agctggcatc caccacggtt tgcggttcag
    29041 acttactggc agcacgcata ttaaccgtca acaccggcga gaagccgctg acatcctgac
    29101 gtacgacctg aaaagtgtcg ataatgatgg catccggatt agtgacttta tcccagccct
    29161 taccttcaca ggatgtcgca ccgcgtagcg tttccagcac atgctccttc agacgaaatc
    29221 caatctggtc agactctttt accggttcgc gatcccagat accgttactg ttcgcatccc
    29281 actgcacaat gatacagtca ccctgtccga caatttccag cccttcgccg gtacagctgc
    29341 catgacaata acccgccctc tggagatgct tcgcgacggt aaatacccgc agccagattt
    29401 catcttccag cgccagctta cgggtgctcg ttaaactttc acgctgtaac gcaggcagaa
    29461 agcgtgcagc ccccagcaac aatacgctac tgatcgccat agcaatcaac acttccagca
    29521 gagaaaaacc ttgctctttt acaggcatcc ttctgtttct ccttgctgac aaagccggag
    29581 tcttccccac ggcgaaacca ccagccacca ctcgcccgtt gagtttttga agcgaatatg
    29641 cccggcccat gcggtattgc gcaggccaaa gaaagcaagc gaaggtgtca ggtcgctcat
    29701 ttcgacttcg ggccagcgtg gcacaaagac caatggtgaa ctgccatgac aggtattggc
    29761 cccagcagcg gaactcacaa ggcaccataa cgtcccctcc ctgataacgc tgatactgtg
    29821 gtcgcggtta tgccagttgg catcttcacg taaatagagc aaatagtccc gcgcctggct
    29881 ggcggtttgc catagccgtt gcgactgctg ccagtattgc cagccataga gtccacttgc
    29941 gcttagcatg accaaaatca gcatcgcgac cagcgtttca atcagcgtat aaccacgttg
    30001 tgttttcatg ccggcagtat ggagcgagga gaaaaaaaga cgagggccag tttctatttc
    30061 ttcggcgcat cttccggact atttacgccg ttgcaggacg ttgcaaaatt tcgggaaggc
    30121 gtctcgaaga atttaacgga gggtaaaaaa aaccgacgca cactggcgtc ggctctggca
    30181 ggatgtttcg taattagata gccaccggcg ctttaatgcc cggatgtgga tcgtagcctt
    30241 caatctcaaa gtcttcgaaa cggtagtcga agatggattc gggtttacgt ttgataatca
    30301 acttcggcag cggacgcggt tcgcggctta attgcagatg agtttgatcc atatgattgc
    30361 tgtacaggtg cgtgtcgccc cccgtccaga caaaatcgcc cacttccaga tcacactgct
    30421 gcgccatcat atgcaccagt aacgcgtagc tggcaatgtt aaacggcagg ccgaggaaaa
    30481 cgtcacagga gcgctgataa agctggcaag agagtttgcc gtctgccaca tagaactgga
    30541 agaacgcatg gcacggtgcc agcgccattt tatccagttc gcctacgttc cacgctgaaa
    30601 caataatgcg gcgcgaatcc gggtcgtttt tcagctggtt cagtaccgta gtgatctggt
    30661 caatatgacg accatccggc gttggccagg cgcgccactg tttaccatac actggcccga
    30721 ggtcgccgtt ttcatcggcc cattcgtccc agatggtgac attgttttcg tgtagataag
    30781 caatgttagt gtcgccctgc agaaaccaca gcagttcatg gatgatggaa cgcaggtggc
    30841 aacgtttagt tgtcaccagc gggaatccat cctgcaggtt aaaacgcatc tgatgaccaa
    30901 aaatggaaag cgttccggtt ccggtacggt cgtttttctg tgtgccttcg tcgagcactt
    30961 tttgcatcag ttctaaatac tgtttcatgg ttcctcagga aacgtgttgc tgtgggctgc
    31021 gacgatatgc ccagaccatc atgatcacac ccgcgacaat catcgggatg gaaagaattt
    31081 gtcccatgct gatgtactgc acccaggcac cagtaaactg cgcgtcgggc tggcggaaaa
    31141 actcaacaat gatgcgaaac gcgccgtaac caatcaggaa caaacctgag acagctccca
    31201 ttgggcgcgg tttacgaata tacaggttga ggataataaa cagcactaca ccttccagca
    31261 acagctcgta aagctgtgat gggtggcgcg gcagcacacc gtaagtatcg aaaatggatt
    31321 gccactgcgg gttggtttgc agcagcaaaa tatcttctgt acgggagcca gggaacagca
    31381 tggcaaacgg gaagttcggg tcaacgcggc cccacaattc accgttaata aagttgccca
    31441 gacgcccggc accaagacca aacggaatga gtggtgcgat aaaatcagag acctggaaga
    31501 aggaacgttt agtacggcgg gcgaagataa tcatcaccac gataacgcca atcaggccgc
    31561 cgtggaaaga catgccgccg tcccagacac ggaacagata cagcggatcg gccataaact
    31621 gcgggaaatt gtagaacaga acataaccaa tacgtccacc gaggaagacg ccgaggaagc
    31681 ccgcatagag taagttttca acttcatttt tggtccagcc actgcccgga cgattcgccc
    31741 gtcgtgttgc cagccacatt gcaaaaatga aacccaccag atacatcagg ccgtaccagt
    31801 gaagcgccac gggtcctatt gagaaaatga ccggatcaaa ctccggaaaa tgcagatagc
    31861 tactggtcat ctgtcaccac aagttcttgt tatttcgctg aaagagaaca gcgattgaaa
    31921 tgcgcgccgc aggtttcagg cgctccaaag gtgcgaataa tagcacaagg ggacctggct
    31981 ggttgccgga taccgttaaa agatatgtat atgatccgcg ctataaccct ccgcgaatca
    32041 gcccgcccat gccgcgacgc tccataaagg ctgcaacctg atggcgaact tcggtcgcca
    32101 gttgcgcttc cagactacgc tgcgcaagat tttctgcgtc ggcataatca atgcgccgca
    32161 gcaggtattt tacccgcgct acagaacgtc cgttcataga cagatggcga tacccaagcc
    32221 caatgaggat tgccacgcac atgggatcgc ccgccatttc accgcacaaa cggagatcga
    32281 ttccatgtat ttccgcttcc cgggcgatca tcgccagagc tcgtaacatt gcaggatgaa
    32341 gactgtcata aatgttcgcc acccgggtat tgttgcgatc aacggccaga atgtattgag
    32401 tcagatcgtt ggtgccaaca gagatgaaat cgacccgctt tgccagatgc ggcagcataa
    32461 ataccattga cggcacttcc agcatgatgc cgatacgtgg tttgggaatt tcgtaaccga
    32521 tcatctcctc gacttcacgt ccggcacgtt caatcaggcg gcgtgcttca tcaacttcat
    32581 cgaggcttgt gaccatcggc aacagaatat tcaggttgcc cgtagcggca ttagcacgca
    32641 gcatcgcccg cacctggatc aagaagatct ccggctgatc gagcgtaatg cgaatcccac
    32701 gccaacccag gcatggattc tcttcgctga tcggcatgta aggcagctgc ttatctgctc
    32761 cgacatccag cgtacgcaag gtgacgggtt tatcattgaa catttgcagc atcccctgat
    32821 actgcgccac ctgttcttct tccgacggga aaccactttg cagcatgaat gggatttcag
    32881 tgcgataaag accgatgcca tcaatacggc tgcccagttt ttcttcatgt tccgggctta
    32941 aaccagcatt gagcatgact tttatacgct caccgctttt taactgcgcg ggtaaattga
    33001 cgtcatcttc cgccagacgg ctaagctcaa tctcttcact aattagccgc tgatattctt
    33061 gcagcagtac cggctccgga tcgaccagca attcaccgcg atagccatca acgatcagcg
    33121 tcctacgatg cagcaccgaa ggctgaatat ccgcgcccat cacggtaggg atccccagcg
    33181 cacgtaccat gatcgcagca tgggagttgg ctgcgccatc tcgcacgaca acaccgacta
    33241 agcgatcctg gggcagctca gcaagagttg tcgctgacag ttcatctgcc accagaatga
    33301 aacgttccgg ccaggcgttc ggcccttgat tagcgtcatc aagatgaaac agcaatcgct
    33361 gacccagcgc acgtaaatcg ccagcccgct ctttgagata gttatcgctt agcgcggcaa
    33421 actgttcggc aaatttttca atgaccgttt ttaccgccca ctctgccacc gagcctttat
    33481 caacctcggc aaacaattcg cgacgcagcc gggtgtccga aagtaggtgc gagtaaagat
    33541 cgaaaatagc cgccgtttct ttttgtgctc cggcggcaaa gcgtttgctg tagcggcgaa
    33601 actcgtttgc cgcttcttcc agcgccccgg tcagtcgttc gcgttccaga gccggatcca
    33661 gcgttgatgc ctgatacacc tgttccatta aaggtaacgt ggcatcctgc cagccttcgg
    33721 caatcgccac accaggtgct gccggtaatg cgcggattcg cgtctggcga tattgcccaa
    33781 acagggcagt caactgcgac tgagaaagaa tagctgccat ctgggtggca agcgtcacca
    33841 ggaaggattc ttcactttcg tcatactggc gcaactctcg ttgctgtacc accagtacac
    33901 caagcaactg gcgacgttga ataattggta cgcctaaaaa cgcgcggaaa cgttcttctt
    33961 ttacggaggg gatgtatttg aagctggggt gcttttgcgc atctgcaagg tttatcggtt
    34021 ccgccagcct gccaaccagg ccgacgatcc cttcatcaaa cgcgagcgtt acagtgcgac
    34081 cgcgtggttt tttcagcccc cgggtcgcca tcaggtagta acaacgtcga tcatgatcgg
    34141 ccaggtagac cgaacagacc tcggtatcca tcgcaagaca gatgtcggta accagaatat
    34201 ttaacgcctc attcaggcgt ggtgcgctgg ctaccttttc gactatttcg cgcaggcgag
    34261 tgagcataat tggcgtgact taacctcttt tacgtcgata agcagatgcg ttttgtggtt
    34321 ttggcgtatt ttcctgcagt gacatcacca cactcgcgaa ctctttcatt accctacggt
    34381 agacatcacg tttaaatgac accacctgtc tgaccggata ccagtaactt acccatcgcc
    34441 agccgtcaaa ctctggtgta ctgctggttt gcatattgat ttctgcatcg ccgctcacca
    34501 gctgcaagag aaaccatttt tgtttttggc cgatacaaac cggcttcgtg tcccaacgca
    34561 ccaaacgttt cggtaatttg tagcgcaacc agttacgcgt tgaagcaagg attcgaacgt
    34621 ctttgcggct taatcctact tcttcaaaca attcacggta catcgcctgc tctgcagatt
    34681 ctccggggtt gattccgcct tgcggaaatt gccaggagtg ctgaccaaat cgccgggccc
    34741 acattacctg cccctggcga ttacaaatca cgatacctac gtttgggcgg tagccatcgt
    34801 catcaatcac cggactacct caaaataaag ctttatatac gaatgattgt ttcatactcc
    34861 aggaagacgg taaaccactc tctgcagggc attacacact aataacaatt gaataactca
    34921 cagttatgtg cagagttata aacagaggaa ggggtggata gccgttttta ttcacctttt
    34981 ctgtggatag agttgtgaag aactacggaa ttactacggg aaaacccgga gcattctgaa
    35041 taagcccgat atagacattt taaaatattc attattagtc aatgcattac atgtaatttg
    35101 agtaatgaaa aagagtaatt cgtgacccag gtcacacctc tcatttacgg gttggcgaaa
    35161 gatcaaccaa tgccgtattt atccacagaa tgtgccacta agttaagcac tgaaccacta
    35221 aaaactggag tttcgtcgca cgtcaaggct gtaaatggaa acagtagtgg aggtttttca
    35281 cagttatccc agctttctgt ggataacatg gtgtaagatc ctgtttattt tcagtgacca
    35341 gatttggaaa acctattgca gtgttgcgca actcgattac cggcagccta aaaagcaata
    35401 taaatcagag aattgaacaa cacatgtgga aaaagttaca ctgcgaatat tcggcacata
    35461 attgctgttt gttttttaac caaggtatca tgacatgtcc caacctcgcc cactgctctc
    35521 tcctcccgaa actgaagaac agttgttagc gcaagcacag caactttctg gttatacatt
    35581 gggagaactg gcggcacttg ccgggctggt tacgccagag aatttaaaac gcgataaggg
    35641 ctggattggc gtgttactgg agatctggct aggtgccagc gcagggagta aacctgagca
    35701 agattttgct gctctgggcg tggaacttaa aactatccct gtggatagtc ttggtcgtcc
    35761 gctggaaaca acattcgttt gtgttgcccc gttaacgggt aatagcgggg tgacctggga
    35821 aaccagccat gtgcgccaca agctcaaacg cgtactgtgg ataccggttg aaggcgagcg
    35881 cagcatcccg ctggcgcagc gtcgcgtagg atcaccgttg ctgtggagcc cgaatgaaga
    35941 ggaagaccga cagctacgcg aagactggga agaattaatg gatatgattg ttctcggtca
    36001 ggttgagcgg atcaccgctc gtcacgggga atatttacag ataagaccga aagcagcgaa
    36061 tgcgaaagcg cttaccgaag ccattggtgc ccggggcgaa cggattctga cgctgccacg
    36121 cggcttttat ttgaagaaga atttcaccag tgcactactg gcccgtcatt ttctgatcca
    36181 gtagccatcg ctttgacctg ccgctttccg ggcatataat taccgcttca tttttttggc
    36241 agggcttttt agatgttatt tgcatggata accgatccta acgcctggct tgcgctcggt
    36301 acgctgacgc tgctggagat cgttcttggg atcgacaata ttattttcct ttctctggtg
    36361 gtggcaaagc ttcccacagc acaatgtgct catgcgcgcc gtctggggtt ggcgggagcc
    36421 atggttatgc gtctggcgct gctggcatca atcgcctggg ttacgcgcct gacaaatccg
    36481 ctttttacaa tattcagtca ggaaatttct gcccgtgatt tgattctgct tctgggtggc
    36541 ttgttcctta tctggaaagc cagcaaggaa atccacgaat ccattgaagg tgaagaagaa
    36601 gggctgaaaa cacgcgtttc atcattcctc ggcgctatcg tgcagattat gctgctggat
    36661 attatcttta gcctcgactc ggtgattacg gctgtgggtc tgtcagatca cctgtttatt
    36721 atgatggcag ccgtggtgat tgccgtaggc gtgatgatgt tcgccgcgcg ctcgattggt
    36781 gattttgtcg aacgccatcc ttcggtaaaa atgctggcgc tctctttcct gattctggtc
    36841 ggctttaccc tgattctgga aagtttcgac atccacgtac cgaaaggtta catctacttc
    36901 gcgatgttct tctctattgc ggttgaaagc ctcaacctga ttcgcaacaa aaagaacccg
    36961 ctctgatact ccgtacgctc tcctgcggga gggcgtactc actcctgtta ccctcacagt
    37021 ttaagatttt cctgctttca agatatataa cgtcggttta taaacagact attatcatag
    37081 gtgggccaaa tgaggataac cggatgaaaa aatgggcagt aataatttct gcagtcggac
    37141 tggcgtttgc tgtttccggg tgttccagtg attacgtcat ggcgaccaaa gatggccgta
    37201 tgattttgac cgatggaaaa cctgaaattg atgatgatac cgggctggtg agttatcacg
    37261 atcagcaagg taacgcgatg caaattaacc gtgatgatgt ttcgcaaatt attgaacgtt
    37321 aacaaataag gtcagcatc
//
LOCUS       DBANUU010000036        37165 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000036 DBANUU010000000
VERSION     DBANUU010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.036"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(134..274)
                     /gene="yjjY"
                     /locus_tag="ACIE68_001710"
     CDS             complement(134..274)
                     /gene="yjjY"
                     /locus_tag="ACIE68_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFX5908039.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            370..1086
                     /gene="arcA"
                     /locus_tag="ACIE68_001711"
     CDS             370..1086
                     /gene="arcA"
                     /locus_tag="ACIE68_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFX5908040.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1145..2497)
                     /gene="creD"
                     /locus_tag="ACIE68_001712"
     CDS             complement(1145..2497)
                     /gene="creD"
                     /locus_tag="ACIE68_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFX5908041.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDV
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(2555..3979)
                     /gene="creC"
                     /locus_tag="ACIE68_001713"
     CDS             complement(2555..3979)
                     /gene="creC"
                     /locus_tag="ACIE68_001713"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFX5908042.1"
                     /translation="MHIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3979..4668)
                     /gene="creB"
                     /locus_tag="ACIE68_001714"
     CDS             complement(3979..4668)
                     /gene="creB"
                     /locus_tag="ACIE68_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFX5908043.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4681..5154)
                     /gene="creA"
                     /locus_tag="ACIE68_001715"
     CDS             complement(4681..5154)
                     /gene="creA"
                     /locus_tag="ACIE68_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFX5908044.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5365..6234
                     /gene="robA"
                     /locus_tag="ACIE68_001716"
     CDS             5365..6234
                     /gene="robA"
                     /locus_tag="ACIE68_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFX5908045.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6231..6878)
                     /gene="gpmB"
                     /locus_tag="ACIE68_001717"
     CDS             complement(6231..6878)
                     /gene="gpmB"
                     /locus_tag="ACIE68_001717"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFX5908046.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6930..7451
                     /gene="yjjX"
                     /locus_tag="ACIE68_001718"
     CDS             6930..7451
                     /gene="yjjX"
                     /locus_tag="ACIE68_001718"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFX5908047.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(7536..7862)
                     /gene="trpR"
                     /locus_tag="ACIE68_001719"
     CDS             complement(7536..7862)
                     /gene="trpR"
                     /locus_tag="ACIE68_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFX5908048.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(7952..9889)
                     /gene="sltY"
                     /locus_tag="ACIE68_001720"
     CDS             complement(7952..9889)
                     /gene="sltY"
                     /locus_tag="ACIE68_001720"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFX5908049.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPRLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPSYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10100..11767
                     /gene="ettA"
                     /locus_tag="ACIE68_001721"
     CDS             10100..11767
                     /gene="ettA"
                     /locus_tag="ACIE68_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFX5908050.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11874..12041)
                     /gene="nadS"
                     /locus_tag="ACIE68_001722"
     CDS             complement(11874..12041)
                     /gene="nadS"
                     /locus_tag="ACIE68_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFX5908051.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(12075..13307)
                     /gene="nadR"
                     /locus_tag="ACIE68_001723"
     CDS             complement(12075..13307)
                     /gene="nadR"
                     /locus_tag="ACIE68_001723"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFX5908052.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(13328..14710)
                     /gene="radA"
                     /locus_tag="ACIE68_001724"
     CDS             complement(13328..14710)
                     /gene="radA"
                     /locus_tag="ACIE68_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFX5908053.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14759..15727)
                     /gene="serB"
                     /locus_tag="ACIE68_001725"
     CDS             complement(14759..15727)
                     /gene="serB"
                     /locus_tag="ACIE68_001725"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFX5908054.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLCS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            15833..16477
                     /gene="ytjB"
                     /locus_tag="ACIE68_001726"
     CDS             15833..16477
                     /gene="ytjB"
                     /locus_tag="ACIE68_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFX5908055.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16505..17521
                     /gene="lplA"
                     /locus_tag="ACIE68_001727"
     CDS             16505..17521
                     /gene="lplA"
                     /locus_tag="ACIE68_001727"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFX5908056.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAISETFFAHYGERTEA
                     EIISPDKTPDLPNFTETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRTQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWIA
                     GAVR"
     gene            complement(17522..18853)
                     /gene="yjjJ"
                     /locus_tag="ACIE68_001728"
     CDS             complement(17522..18853)
                     /gene="yjjJ"
                     /locus_tag="ACIE68_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family toxin
                     YjjJ"
                     /protein_id="HFX5908057.1"
                     /translation="MSELTDLLLQGPRSAPELRQRLAISQATFSRLVAREDRVIRFGK
                     ARATRYALLRPYRGIERIPVWRVDDAGKAHKFADIQLCWPQGSCLVTGGKGDEQWFDG
                     LPWYLTDLRPQGFLGRAWGRNLAGQLNLTEDIRLWQEEDVLYALTVFNGEYTGGWLVG
                     ERNYQRWITAPRPAAIPLDQKLTRYEQLAHDALAGEIVGSSAGGEQPKFTCYVQTPSG
                     NKHVLVKFTVPQQTAVSQRWGDLLIAESIAAQILCDGGIHAIESTVLVTSNRQVFLEA
                     QRFDCKGNDGRLPIVSLEAVQSEFISSPGSWPEAMRRLCEQQFVTHQSVVQTEVIWAF
                     GRLIANSDMHAGNLSFYLSEPPFALTPVYDMLPMAYAPNSAGMLRDAAIEVKFDLNVS
                     KSAWLTAIPLAQQFWQMVAGDPRISEAFRHIAQEMPEKIRQIEGKVARMGG"
     gene            complement(19020..19739)
                     /gene="deoD"
                     /locus_tag="ACIE68_001729"
     CDS             complement(19020..19739)
                     /gene="deoD"
                     /locus_tag="ACIE68_001729"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFX5908058.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(19796..21019)
                     /gene="deoB"
                     /locus_tag="ACIE68_001730"
     CDS             complement(19796..21019)
                     /gene="deoB"
                     /locus_tag="ACIE68_001730"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFX5908059.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(21071..22393)
                     /gene="deoA"
                     /locus_tag="ACIE68_001731"
     CDS             complement(21071..22393)
                     /gene="deoA"
                     /locus_tag="ACIE68_001731"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFX5908060.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLTSGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSDMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(22560..23339)
                     /gene="deoC"
                     /locus_tag="ACIE68_001732"
     CDS             complement(22560..23339)
                     /gene="deoC"
                     /locus_tag="ACIE68_001732"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFX5908061.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            23598..25148
                     /gene="yjjI"
                     /locus_tag="ACIE68_001733"
     CDS             23598..25148
                     /gene="yjjI"
                     /locus_tag="ACIE68_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFX5908062.1"
                     /translation="MSTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAALKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            25120..25983
                     /gene="yjjW"
                     /locus_tag="ACIE68_001734"
     CDS             25120..25983
                     /gene="yjjW"
                     /locus_tag="ACIE68_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFX5908063.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELARFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(26398..27180)
                     /gene="yjjV"
                     /locus_tag="ACIE68_001735"
     CDS             complement(26398..27180)
                     /gene="yjjV"
                     /locus_tag="ACIE68_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFX5908064.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYAVFSVRG"
     gene            complement(27177..28250)
                     /gene="yjjU"
                     /locus_tag="ACIE68_001736"
     CDS             complement(27177..28250)
                     /gene="yjjU"
                     /locus_tag="ACIE68_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFX5908065.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            complement(28372..28533)
                     /gene="ytjA"
                     /locus_tag="ACIE68_001737"
     CDS             complement(28372..28533)
                     /gene="ytjA"
                     /locus_tag="ACIE68_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFX5908066.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(28660..29265)
                     /gene="osmY"
                     /locus_tag="ACIE68_001738"
     CDS             complement(28660..29265)
                     /gene="osmY"
                     /locus_tag="ACIE68_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFX5908067.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(29658..31247)
                     /gene="prfC"
                     /locus_tag="ACIE68_001739"
     CDS             complement(29658..31247)
                     /gene="prfC"
                     /locus_tag="ACIE68_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFX5908068.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(31338..32015)
                     /gene="yjjG"
                     /locus_tag="ACIE68_001740"
     CDS             complement(31338..32015)
                     /gene="yjjG"
                     /locus_tag="ACIE68_001740"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFX5908069.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(32030..32476)
                     /gene="rimI"
                     /locus_tag="ACIE68_001741"
     CDS             complement(32030..32476)
                     /gene="rimI"
                     /locus_tag="ACIE68_001741"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFX5908070.1"
                     /translation="MNTISSLETTDLPAAFHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(32445..32858)
                     /gene="holD"
                     /locus_tag="ACIE68_001742"
     CDS             complement(32445..32858)
                     /gene="holD"
                     /locus_tag="ACIE68_001742"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFX5908071.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            32961..33992
                     /gene="rsmC"
                     /locus_tag="ACIE68_001743"
     CDS             32961..33992
                     /gene="rsmC"
                     /locus_tag="ACIE68_001743"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFX5908072.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGSGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            34293..34379
                     /locus_tag="ACIE68_001744"
     tRNA            34293..34379
                     /locus_tag="ACIE68_001744"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34327..34329,aa:Leu,seq:cag)
     gene            complement(34418..34654)
                     /gene="yjjZ"
                     /locus_tag="ACIE68_001745"
     CDS             complement(34418..34654)
                     /gene="yjjZ"
                     /locus_tag="ACIE68_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFX5908073.1"
                     /translation="MLQRMLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIVNLNQG"
     gene            34795..35583
                     /gene="fhuF"
                     /locus_tag="ACIE68_001746"
     CDS             34795..35583
                     /gene="fhuF"
                     /locus_tag="ACIE68_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFX5908074.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLRAMTLAQWSSPNTLSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVTPEHFHVEFHETGRAACFWVDVCEDKNATLHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(35621..36298)
                     /gene="bglJ"
                     /locus_tag="ACIE68_001747"
     CDS             complement(35621..36298)
                     /gene="bglJ"
                     /locus_tag="ACIE68_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFX5908075.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(36256..36981)
                     /gene="yjjQ"
                     /locus_tag="ACIE68_001748"
     CDS             complement(36256..36981)
                     /gene="yjjQ"
                     /locus_tag="ACIE68_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFX5908076.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
BASE COUNT         8799 a        10202 c         9380 g         8784 t
ORIGIN      
        1 gattattaaa ataatgtgac gaaagctagc atttagatac gatgatttca tcaaactgtt
       61 aacgtgctac aattgaactt gatatatgtc aacgaagcgt agttttattg ggtgtccggc
      121 ccctcttagc ctgttatgtt gctgttaaaa tggttaggat gacagccgtt tttgacactg
      181 tcgggtcctg agggaaagta cccacgacca agctaatgat gttgttgacg ttgatggaaa
      241 gtgcatcaag aacgcaatta cgtactttag tcatgttacg ccgatcatgt taatttgcag
      301 catgcatcag gcaggtcagg gacttttgta cttcctgttt cgatttagtt ggcaatttag
      361 gtagcaaaca tgcagacccc gcacattctt atcgttgaag acgagttggt aacacgcaac
      421 acgttgaaaa gtattttcga agcggaaggc tatgatgttt tcgaagcgac agatggcgcg
      481 gaaatgcatc agatcctctc tgaatatgac atcaacctgg tgatcatgga tatcaatctg
      541 ccgggtaaga acggtcttct gttagcgcgt gaactgcgcg agcaggcgaa tgttgcgttg
      601 atgttcctga ctggccgtga caacgaagtc gataaaattc tcggcctcga aatcggtgca
      661 gatgactaca tcaccaaacc gttcaacccg cgtgaactga cgattcgtgc acgcaacctg
      721 ctgtcccgta ctatgaatct gggtactgtc agcgaagaac gtcgtagcgt tgaaagctac
      781 aagttcaatg gttgggaact ggacatcaac agccgttcgt tgatcggccc tgatggcgag
      841 cagtacaagc tgccgcgcag cgagttccgc gccatgcttc acttctgtga aaacccaggc
      901 aaaattcagt ctcgtgctga actgctgaag aaaatgaccg gccgtgagct gaaaccgcac
      961 gaccgtactg tagacgtgac gatccgccgt attcgtaaac atttcgaatc tacgccggat
     1021 acgccggaaa tcatcgccac cattcacggt gaaggttatc gcttctgcgg tgatctggaa
     1081 gattaatcgg ctttaccacc gtcaaaaaaa cggcgctttt tagcgccgtt tttatttttc
     1141 aaccttattt ccagatacgt aattcatcgt ccgttgtaac ttctttactg gctttcattt
     1201 tcggcagtga aaacgcatac cagtcgatat tacgagtcac aaacatcatg ccggccagcg
     1261 ccaccaccag cacactggtt cccaacaaca gcgcgctatc ggcagagttg agcagtcccc
     1321 acatcacacc atccagcaac aacagcgcga gggtaaacaa catgctgttg cgccagcctt
     1381 tcaataccgc ctgcaaataa ataccgttca ttaacgcccc aatcagactg gcgattatcc
     1441 atgccacggt aaaaccggta tgttcagaaa gcgccagcaa gagcaaataa aacatcacca
     1501 atgaaagccc caccagcaaa tattgcattg ggtgtaagcg ttgcgcggtg agcgtttcaa
     1561 aaacaaagaa cgccataaaa gtcagcgcaa tcagcagaat ggcatactta gtcgcccggt
     1621 cagttaattg gtattgatct gctggcgtcg tcactgcgac gctaaacgcg gggaagtttt
     1681 cccagccggt atcattgcct gaagcaaaac gctcaccgag attattagca aaccagctgc
     1741 tttgccattg cgcctgaaaa cctgactcgc taacttcccg tttggctggt agaaaatcac
     1801 ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat ttcgctatta cgcccggcag
     1861 gcaccacaga aagatcgccg gtaccgctta aattcagtgc catattcagc tgcaggttct
     1921 gcttgcgcca gtccccttcg ggtaaaggga tatgcacgcc ctgcccgcct tgctctaacc
     1981 cggtaccggg ttcaatggtc agcgctgttc cgttaacttc aggcgctttc accacaccaa
     2041 taccacgcgc atccccgacg ctaatcacaa taaagggctt gcctaagatg atatttggcg
     2101 cgttgagctc gctaagacgc gaaacatcga aatcggcttt tagcgttaaa tcactgtgcc
     2161 agacctgacc ggtataaatc cctatcttgc gttcttccac gttctgattg ccatcaacca
     2221 tcaatgactc aggtaaccaa aaatggataa aacttcgttt ccgctccacg gttttatcct
     2281 cttcctgcac cgtataaagc tcggtcacag gaatagcgat aagcggccca acgagttttt
     2341 gcggcccgct ggtactttgg cgaatcacat cttccacatc gctacggtaa tcggcacgtt
     2401 cgacaatcac ttgccgaatc agcattatcg gaatcaacaa cagcaatact gcaccaaaca
     2461 aggtggtcat tttccagaac aggggggatt tcaacatagt cttctccttt gcaatggcag
     2521 caggatacga aggctatgtg ggaagaattt gaagctatgt gaagtgacgg tgaagtcgta
     2581 gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt gacttcgccg ttaaacaaac
     2641 gggcgacctc actgacgaac gccaacccca gaccgctgct tttttgccca tttgcacgcg
     2701 gcaaagagta aaagcgttca aaaatgcgtg acagcgcgta gtcaggaata ccactaccgg
     2761 tatccagcac cttaagcgtg acgtgttcct gatccacttc ggcgcttagc gttatacgac
     2821 cgctctcggg ggtaaaatcg atggcgttat ccagtaaatt ccccagcgcc tgatccagta
     2881 acgccggttc agcagcaaca ttaacctcgg tgggcgtaac atgtagagtg atgttttttt
     2941 ctgccaactg cacggtgcgc gcttcgctga cgcggcggaa taatgccgcc acatcaacag
     3001 cagtcaaaac gacttcctga cgattctcca gtcttgcctg gcgtagtaac gtttccacca
     3061 gtgcctgcat tcgcgcattt tgcgtcagaa tgttgtcggt aaaacgagcc accacttccg
     3121 gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat cgccgccagt gggcttttta
     3181 gctcatgggt taacgcataa acatactgct caatatagtt tttcccttcc agcttcacgc
     3241 gcatactttc cagcgcctgc gcgagtttac gcagttcgct actaccgaga tcgggaagag
     3301 gaacgggctt attgtcagtg acggaatcag catagcgagt gagccgggca atggagcggt
     3361 tgatccacca aaccatgcct gcgccaatca ccagtgcgat ccccaacaaa atggcgctgg
     3421 cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc catcgccgcg ttcggtttgc
     3481 ctacgctcaa aacgccaata agccgcgagc cgtccataat cggcgcggca acatacatca
     3541 ccgaactttc gggatcggca ggattttgca acgtgctacg cgcaccatac tgaccacgca
     3601 acgttagcca gacgtcattc cagcgcgaat aatcctgtcc aacggcttta tttgccgaat
     3661 cgaacaatac tttgccctgc gcatcggtca tatagacatg atattcattg cgcactttgt
     3721 taatgccacc aatattggcg cgaaacgggc gatgttgtag ctgattaaac gcctgcgcca
     3781 gttgcccatg cgttgggtcc ccagagagca aatcgggacg cgccagctcc gccagtaacg
     3841 ttgccgtgtc gatcaacgtc ccctccgttg ctcttcgcac gcccggttta acttctttga
     3901 caaaaatagc cagtacgaac caggccgcca ccgccaccag taaaaaatag cccagcagca
     3961 accgcatgcc gatatgcatt acaagcccct caggctatat cccatgccgc gatgagtatt
     4021 aatcggtgaa agatcggggt tgatggcgcg cagcttggca cgcagcgttt taatgtgggt
     4081 atcgacggtg cgatcgtagg tgtcctgcgc atcttcccac acgctatcca ttagttgctg
     4141 gcgggaccag acgcggcctg gcgacttgag taacgtcttc agcaataaaa actcataccg
     4201 cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc gcgggttcat tcaattcaaa
     4261 atgaccaata cggatgacgg gagacggcgt cgagaacttc ttcacccgac gcagtaaggt
     4321 gcgcaccctg gcgcacactt cgcggggtga aaacggttta gccacatagt cgtcagcgcc
     4381 aatttccagc ccaagcaggc gatcgacctc ttcacttcgg gccgtcagga acagtacagg
     4441 taacgccgga tggagcgcca gtaactggcg gcacaattca aagccactga tatcaggcaa
     4501 accaacgtcg agaatcatga cgtcgggaac ctgctggcga gctttatcca gcaccggcaa
     4561 acctcgctca aagacctcga cggcaaaacc ttcctgctgc aacatgtaga ccagcgtgtc
     4621 ggctatccct tgctcatctt ccactaacca gaccgtttcc cgttgcataa atcgcctctg
     4681 ttattgccgc cacggcatga caggaaccgc gctaatcgcg tttttcggcg aaccttctac
     4741 aactttgtcg gagtaagcca gataagcgag cgcgttgcgt ttggcgtcat aaaagcgcac
     4801 gacctgtaac gacttaaaga ccagggacgt gcgttttttg aatactacct cgccctgagc
     4861 tttgccgttt ttaatacgat ccgacagttc aatcggcccg acttgctgac aagaaatggc
     4921 cgcatcggag gtatcttctg ccaggcccaa tcccccttta ataccaccgg ttttcgcccg
     4981 gctcacataa caggtgacat ttttcacatc gggatcgtca aaggcttcca caacaatttt
     5041 gtgatccggg ccgatcattt taaatacggt gtcgaccgaa ccaatctctt ctgcatgagc
     5101 caatggcccc agcattatca ggcttaaaga caggatcaaa tgcttgtatt tcatattgtt
     5161 accattttta tctattacac gagcaattag ttcgtcacgg taaataacat attgattaag
     5221 aacaaaatct caattctttt atttccgtca agccctaaaa catactctac taagaaaaaa
     5281 acactgaatg ctaaaacagc aaaaaatgct attatccaat tacctgatgt caggtgctcg
     5341 ttgttgaaag gatgaggata ttttatggat caggccggca ttattcgcga ccttttaatc
     5401 tggctggaag gtcatctgga tcagcccctg tcgctcgaca atgtagcggc gaaagcaggt
     5461 tattccaagt ggcacttaca gagaatgttt aaagatgtca ctggccatgc tattggcgcg
     5521 tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac tgcgcgtccg
     5581 attctggaca tcgcgctgca ataccgcttc gactctcaac agacatttac ccgcgcattc
     5641 aagaagcagt ttgcccagac tcctgcactt taccgccgtt ctcctgaatg gagcgccttt
     5701 ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc cagagcacaa atttgtcacc
     5761 ctggaagata cgccgctgat tggtgttacc cagagctact cctgttcgct ggagcaaatc
     5821 tctgatttcc gccatgaaat gcgttatcag ttctggcacg attttctcgg caacgcgccg
     5881 accattccgc cggtgctcta cggcctgaat gaaacgcgtc cgagtcagga taaagacgac
     5941 gaacaagagg tattctatac caccgcgtta gcccaggatc aggcagatgg ctatgtactg
     6001 acagggcatc cggtgatgct gcagggcggc gaatatgtga tgtttaccta tgaaggtctg
     6061 ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa cgtgcatgcc aatgctcaat
     6121 ctgacgcgcc gtaaaggtca ggatattgag cgatactacc cggcagaaga cgccaaagcg
     6181 ggagatcgcc caattaatct gcgctgtgaa ctgctgattc ctatccgccg ttaacgctgc
     6241 agctcatcta atgcaggggc gtccagatgc gagatgtccc ctgcggtttc aacgacccag
     6301 ccagacgcca gccacaggct ttcctgataa tccacgcgcg aaatagaaca gttacgtaga
     6361 cgtaagcggc gctctgccca tgctggtaat ccgagaatcg tactcaccag acatcccagt
     6421 gcaataccat ggctcaccag cagcggtcgg cttccctgcg gtaagtcccg gcaagattcc
     6481 agtgccgcat tgacgcgatc gctgagctcc tgcattgact cgccttcagg aatacgcccg
     6541 tcaacggtgc cattgaccag ctgccgacgc cagttctctt cttcttcggt cagagaatcg
     6601 atatgtcttt tttccagcac acccatgttt aattcacgca ggcgagaatc aaagatgatg
     6661 tcacagccgc aggcctgggc gatgatttcc gccgtacgcc gggtgcgtcc taaatcgcta
     6721 ctgataatat gcgtaatgcc aagctctttg gcacgggttg ccacctgcat cgcctgttgc
     6781 tcacctttgg cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc ggcgttccac
     6841 tgcgtttcac cgtggcggac taggtatacc tgtaacatgc tttttttccg tatactgctg
     6901 taacgttaat tttctgagta atgctgatta tgcaccaagt tgtctgtgcg accaccaatc
     6961 ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat cttcggcgaa ggatcctgcc
     7021 atattgcatc cgttgccgtc gagagcggtg taccggaaca accctttggc agtgaggaaa
     7081 cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg tttacttcca gaggctgatt
     7141 tttgggtggc gattgaagct ggcattgatg gcgacagcac tttcagttgg gtggtgattg
     7201 aaaacgccag ccagcgcggc gaggcgcgtt cggcaacttt gccgttacct gcggtgattc
     7261 tggaaaaagt gcgtgaaggc gaggcgcttg gtcccgtgat gtcgcgttat accggtattg
     7321 atgagattgg tcgtaaggaa ggggcgatcg gcgtgtttac cgccgggaaa ctcactcgcg
     7381 ccagcgtcta tcaccaggcg gtgatcctgg cgctgagtcc gtttcacaat gccgtgtatc
     7441 aggcattgta ggacggataa ggcgttcacg ccgcatccgg cattcggtgc acgatgcctg
     7501 atgcgacgct gccgcgtctt atcaggccta caaaatcaat cgcttttcag caacacctct
     7561 tccagccact ggcgcagctc cacgggcgcg gctttcaggc tgttagatcc acgcgtaatc
     7621 gtcgcgatgc ccgcgccgag ttcatttttt aactcacgct gactcatttc gccacgcaac
     7681 agctcttcga caatacgcac gcgagtcccc aacgcttcgc gctcatctgg cgtcagcatc
     7741 aggtttaaca acggtaaatg gagatcgttt tggtaggcat tcttaagcag gtcgacaaaa
     7801 cgtaaccact cctggtgacg ctgttctgcc atcgctgctg aatagggtga ttgttgggcc
     7861 ataatatgtc gccattgtta gcgggggaag caaaatgcct cccccggttg tactcgctaa
     7921 agagtacgat agcatatcat aaacgtgcgg atcagtaacg acgtccccat tccgtggcgc
     7981 tcatcaacgt cggtttatcc cccatgaaat agcggtagta agcgtcataa gccagcacgt
     8041 tcttcacata accgcgcgtc tcggagaacg gaatactctc gacaaatgcc acagcgtcga
     8101 tacgcccggc gctgttgcca agccaggttc gcacccgtcc aggtccggcg ttataagctg
     8161 ctgaggagaa aatacggtta ttgccaaact gctgataaac atattgcagg taactggtgc
     8221 caatgttgat gttcgtttcc ggatccagca attgcccagg gctgctataa ctaggaatag
     8281 agaacatctt caccgtatgg gtcgctgtac caggcataat ctgcatcagg ccgctggccc
     8341 ctaccggtga tttcactttc ggattccagg cgctctcctg acgggcaatc gccatcgcat
     8401 agctttgcgg gatctctttc ccactggtgt aacgtttgaa aagatcgttg taagccagcg
     8461 ggaatcgctc ttccagatga tcccaaagct tcccggcgat cgttgcctga acgctaagat
     8521 cccaccattg gttattgaaa gcataccgag ccagttgagc ctgctctgtt tttgacttgc
     8581 tcttcaccag attggcccac tcgctacgcg cggtgttatc gagattccag tacatcaact
     8641 cgcgcacgcg cgccatctcc ggcccctgag tcagggcgct gtcaacattc tgcggcgctt
     8701 tatcaatctt cagctcatac tcttctccga tgcgttgtgc tgcaaccatc gggtagaaac
     8761 cacgctgttg catgagttga tgcaaaatct cttttgcttc agcttcacgt ccgcgttcca
     8821 gcaataaatc cgcctgccag taacgccatt catctttctc ttttgcttcc atcggcagac
     8881 gcgccagcca ggtattcagg ccgcgacgat cgccggtgcc aagtgccatt cgcacacggc
     8941 gttcaatcaa cgacgtggat tgcgagcgca taatggcgtc atcgcgccat ttcgcctgct
     9001 cgtcggtgac atcgttgccc atcaaacgcc aggcgacgat atcgcgcagc tcctgaatct
     9061 gatcttcatt aagctgctgc gcctgggcaa gcgacgggat catcagccgt gcattttcag
     9121 catcctgccg cgccacactg gcaaacgcca ccgctgccat ttgacgggta aaatcggtcg
     9181 ctccggttgt acgcgcgaag gtcagtaccg tattagggtt gttcgccagt gaaatgattg
     9241 ccgaggcgat agtctggtaa tcggcaggca tctgccctgc cagcactgtt accaggccag
     9301 tgttacccgc tttcatcgcc agacggatac gctctaaata cgccagcgga tcttgtttac
     9361 ctgacgcacg ccagacgcta aataacttgt cacaggcgtt aggctggctc ttgccggtta
     9421 gccacagctc tttcgcccct tgccaggctt cttcactctg cccggtgttc catttcgcat
     9481 agtagtaatt acattgcgct tcggtagttc cgggcttttc cgggctaaag gctaacaagc
     9541 cacgccagtc ttcacgccgc gccagttcat tgacgaaacg agattgcagc gtgcgagcgg
     9601 gaggaagcgt ggggttagcg cgaacaaagt tagtgaccgt caccgccggt tgattcatca
     9661 gatcatcggt gatctggcgg tattccaggt agggataaag cggataatcc ttcagtctag
     9721 gcatcatttg ttcgaccaca tccatctgtc gattatccca ggcctgcttg atttgcgcgt
     9781 aacggctacg ctgctcatcc agtgagtcgg ctcgcgccac gctgctgacc gtcagcagac
     9841 agacaccggc agccaacagc cgccaggtaa cttgtttggc tttttccaca agcgcatcct
     9901 ctaagtgtaa atacatcaat gcagcgtcat gccgcgtaat agatttatgc taaccagtca
     9961 ttgccgttta cgccacgtta cggacacttt tttactttta ctgcgaaggt aatcgaccaa
    10021 ctgggattag gcggggaaaa aggctacact tcgcctttga aaactcatca tcacgataaa
    10081 catagaggcg aagtccaacg tggctcaatt cgtttatacc atgcatcgtg tcggcaaagt
    10141 tgttccgccg aaacgtcata ttttgaaaaa catctctctg agtttcttcc ctggggcaaa
    10201 aattggtgtc ctgggtctga acggcgcggg taagtctacc ctgctgcgca ttatggcggg
    10261 cattgataaa gacatcgaag gtgaagcacg tccgcagcca gacatcaaga ttggttatct
    10321 gccgcaggaa ccgcagctga acccggaaca caccgtgcgt gagtccattg aagaagcggt
    10381 ttctgaagtg gttaacgccc tgaaacgcct ggatgaagtg tatgcgctgt acgccgatcc
    10441 ggatgccgat tttgacaagc tggccgctga acaaggccgt ctggaagaga tcattcaggc
    10501 tcacgacggt cataacctga acgtacagct ggagcgtgcg gcggatgcgc tgcgtctgcc
    10561 agattgggac gcgaaaatcg ctaacctctc cggtggtgag cgtcgtcgcg tggcgttgtg
    10621 ccgcctgctg ctggaaaaac cagacatgct gctgctcgac gaaccgacca accacctgga
    10681 tgccgaatcc gtggcgtggc tggaacgctt cctgcacgac ttcgaaggca ccgtggtggc
    10741 gattactcac gaccgttact tcctcgataa cgttgcaggc tggatcctcg aactggaccg
    10801 cggtgaaggt attccgtggg aaggtaacta ctcctcctgg ctggagcaga aagatcagcg
    10861 tctggcgcag gaagcttcac aagaagcggc gcgtcgtaag tcgatcgaga aagagctgga
    10921 gtgggtacgt cagggtacta aaggccgtca gtcgaaaggt aaagcacgtc tggcacgttt
    10981 tgaagagctg aacagcaccg aatatcagaa acgtaacgaa accaacgaac tgtttattcc
    11041 acctggaccg cgtctgggcg ataaagtgct ggaagtcagc aacctgcgta aatcctacgg
    11101 cgatcgcctg ctgattgatg acctgagctt ctcgatcccg aaaggggcga tcgtcgggat
    11161 catcggtccg aacggcgcgg gtaaatcgac cctgttccgt atgatctccg gtcaggaaca
    11221 gccggacagc ggcaccatca ctttgggtga aacggtgaaa ctggcatcgg ttgatcagtt
    11281 ccgtgactca atggataaca gcaaaaccgt ttgggaagaa gtttccggcg ggctggatat
    11341 tatgaagatc ggcaacaccg agatgccaag ccgcgcctac gttggccgct ttaactttaa
    11401 aggggttgat cagggtaaac gtgttggcga actttccggt ggtgagcgcg gtcgtctgca
    11461 tctggcgaag ctgctgcagg ttggcggcaa catgctgctg ctcgacgaac caaccaacga
    11521 cctggatatc gaaaccctgc gcgcgctgga aaacgccctg ctggagttcc cgggctgtgc
    11581 gatggttatc tcgcacgacc gttggttcct cgaccgtatc gccacgcaca tcctggacta
    11641 ccaggatgaa ggtaaagttg agttcttcga aggtaacttt actgagtacg aagagtacaa
    11701 gaaacgcacg ctgggcgcag acgcactgga gccgaagcgt atcaagtaca agcgtattgc
    11761 gaagtaatgc gtaaaatgcc ggatgcggcg tgaacgcctt atccggccta caaaccaaga
    11821 aatttcaata cgttgtgaat ctccaggcct gataagacgc ggtaagcgtc gcatcaggca
    11881 ttgagcaccg aatgccggat gcggcgtgaa caccttttcc ggtcgacaac agccttagca
    11941 tcctcaattt cgtggcgggt cacccgtgca ggttttttca aaccttgttt aatctcgaca
    12001 gcctcttcca gagaggtttt caactcatca aaaaaactca ttttatacct cgcctttgag
    12061 cagtttcgtc acggttatcc ttgctccccc atcatctccc gcaccagctc cacgcagcgc
    12121 aggaaacggc tgtcgtaatc gtcctcttca acccgcacga attcgatatt gttctcttcc
    12181 agcatctcca ccagcaagtt ctggaactct ttgcgatcca ccgaactgcc gaggctgcgt
    12241 aaaccatccg ccacccacgg cgtgttgttc tccagcagga tcaccagatc gaaacggtat
    12301 tcatcaatca gcgcctgcac gaacggatgc tcacgccctt cgtacttttt gcagaacgcc
    12361 tgggtagtga caaaatcggt gtcaataaac gccactttat tggcgtattt cactgcaaaa
    12421 tcaatgtatt gtgcgtggcc caacgcgatt ttatcgtagt cggaatactg caatgcgatc
    12481 tcatcaccgc cgaggtgtga aaagacataa tcgcggccat attcccacgc actggtggtg
    12541 ttgaagatat tggcaagttt gtttaccagg gtggatttac cgctcgactc gccgccaagg
    12601 atcgccacgg tacgcacaaa aaacggcttc acttcggtag gaatatattc ccagtagcgg
    12661 aacgggtttt cgcggatctg cgcaccgctg atactcataa aggtacgttt cggatcgacc
    12721 agcaccgtct cgatccccag atgttccata tactgtggcg catcggcttc ttccgaggtg
    12781 tagatcagat ccggctggat ccctttttca gccataaact ttttgatacc gttgctccac
    12841 acatcccagc cgtgcggata cggctccatg ccctcttcgt tgaaagcatg aatgcgaata
    12901 tttttctgat atttaaaagt ttgcaataac cagcgcagac gatccggcac ggtcggctgc
    12961 tgcgacatgg cactgtcttc gaacaacgcg cggtcacggg tatcgtcaaa acccataatg
    13021 atatgcagct cgtcaacctg gctacaggcg cgctggataa gatagatatg tccggtatgc
    13081 agcgggtaga acttaccgaa taccacaccg atcgttttct tctgtcgggg aaattcaagc
    13141 cccaaaaaac ggtgcaacgc ctccagcttt tgcgcgctag ggcttttgat tttggcattc
    13201 agtaactggc ttaaataccc tttggtcata ccgctggcat cagctacctg ctgtagcgtg
    13261 cagccctgtt gcttgatggc agttttcagg taatcaaatg acgacatatc tccctccgta
    13321 tctctcatta taagtcgtcg aacacgctaa gcgcgtcgga gagtttttta acgccaaaaa
    13381 tctgcatccc ttccggcgct tttttcggta cgttagccgc cggaacaatc gcccggcgaa
    13441 aaccgtgttt cgccgcttca gagattcgtt cctgcccgct gggcaccggg cggatctccc
    13501 ctgccagccc gacttcacca aacaccacca gatcctgtgg cagcggtctg tcacgcaggc
    13561 tggaaaccat cgccagcagt aacgctaaat cggcactggt ttctgtcacc ttcacgccgc
    13621 cgaccacgtt cacaaacaca tcctgatcgg ccatttgcag accaccgtga cggtgcaaga
    13681 cagccagcag gatcgccaga cggttttgct ccagacctac cgccacgcgg cgcggattcg
    13741 ccatcatcga gtgatcgacc agcgcctgaa tctccaccag caacggacgc gttccttccc
    13801 acaccaccat cacggagctg ccggaggtca cttcatctcc gcgacttaag aaaattgccg
    13861 aagggttgct gacttcacgc agcccctgtt cggtcatcgc gaagacgccc agctcattca
    13921 ccgcgccgaa gcggttttta tggctgcgca aggtgcgaaa acgggagtcg gcatcaccat
    13981 ccagaagcac cgaacagtcg atacagtgtt ccagcacttt agggccagcc agcgagccat
    14041 ctttggttac gtgccccacc atgacaatcg ccacaccgcg cgttttggcg aagcgcgtca
    14101 gataagccgc tgtttcacgc acctgcgcca cactgccagg cgatgattgt atatccgcca
    14161 tatgcatcac ctgaatggag tcaattacca tcagcttcgg ttgctcttct tcggcaatca
    14221 ggcagatctg ttcgatgctg gtttccgaca acatattgag attgtcagtc ggcaggccaa
    14281 ggcgatgagc gcgcattgcc acctgttgca gcgactcttc gccggtgaca tacagcgttt
    14341 tcatctgctg ggccagtttg cacagcgttt gcagtagcag cgtggatttc cccgcaccag
    14401 ggttaccgcc aatcagaatg gcacttcctg gcaccacgcc gccgcctagt acgcggtcga
    14461 actctttaaa tccggtggaa aaacgcggca gctcttcaag gctgatatcg gagagtttct
    14521 ggacttttgc caccccggcg ctaccggcat agccgctgag acgctcgtta cgcgccaccg
    14581 ttggcgacgc agcaagacgc acctcggtga tggtgttcca ggcatgacag gcactgcact
    14641 gcccctgcca gcgcggataa tcggccccgc attcattaca aacaaaggcg cgttttggag
    14701 cttttgccac ggtgtacctc gttaatgctg tgccgcccgc aggatggcgg gcgagcaatt
    14761 acttctgatt caggctgcct gagaggatgc agaatacccc catcaggtca gcgtgacgga
    14821 tggtgacttc cgccttttca ttcacttttg gcttggcatg gtaggcaatc cccagccctg
    14881 ctgctttgat catcggcagg tcattagctc catcgccaat cgccacggtc tgcgccagcg
    14941 ggatttcata ctcctgcgcg aggcgagtca aagttttcgc tttgtactgc gcgtctacga
    15001 tgtcgccgat cacattgccg gtaaatttac cgtccatgat ctccagttca ttggctacca
    15061 cggcggtcag gcgcagcttg tcgcgcagat attcagcaaa gaaagtaaag ccgccggagg
    15121 caatcgccac tttccagccc agcgtttcca gcttgagcac cagttgcgtt aagcctggca
    15181 tcagcggcag attttcacgc acctgttgca gaatattggc gtcagcgcct ttcagcgtcg
    15241 ccacacggct gcacaggctg gcggtaaaat cgagttcgcc gcgcatcgcc cgttcggtta
    15301 cttccgccac catctcgccc gttccggcca gtttggcaat ttcatcaata cattcaatct
    15361 ggatggcggt ggaatccatg tccatcacca gcaaacccgg cgtgcgcagg tgtgggattt
    15421 tccccagcgg cgcgacatcc agctgcgctt cgtgggccag gcgcgtagcc cgggcggtaa
    15481 gcgaacctgc cagacgaatc acctgataat cttccacgca ccaggcggca acaatcacca
    15541 tcgccgcgcc cagtttgctc tggtattggg tcagacgttg tttatccagc ccacgaccat
    15601 acagcagcca gccgctacga cctgcgtggt aatccagtgg catcacttca tcaccactta
    15661 atgaaagagg cagaccaggc cataaagaga catcttcagg caggtcgcac caggtaatgt
    15721 taggcattaa ggctcctgta aaatcgttcg aagcagggaa aataacgcat gaggctacct
    15781 tgtatccatt gcttctggca acattaagtc tcaaattttc aaagggtgga agatggctcg
    15841 cacaaaactg aaattccggc tgcatcgggc agtgattgtc ctgttctgtc ttgccttgtt
    15901 agtggcgctg atgcaggggg catcatggtt tagtcaaaac caccagcgac agcgtaatcc
    15961 gcagctggaa gaactggccc gcaccctggc gcggcaggtg acgctgaacg ttgcgccgct
    16021 gatgcgtacc gactcgccgg atgaaaaacg tattcaggcg atcctcgatc agttaacgga
    16081 tgaaagccgc atcctcgacg cgggcgtgta tgacgaacaa ggcgatctta tcgcccgttc
    16141 tggcgaaagc gtcgaagtgc gtgaccggct ggcgctcgac ggtaaaaaag cgggcggcta
    16201 ttttaaccag cagattgtcg aacccattgc agggaaaaat ggtccgcttg gctatctgcg
    16261 cctgacgctc gacacccata cgctcgccac cgaagctcaa caggtggata acaccactaa
    16321 cattttacgc ctgatgttgt tgctctcgct ggcaatcggc gtagtgctga cccgcacgct
    16381 gctacagggt aaacgcaccc gctggcagca atcccccttc ctgttaaccg ccagcaaacc
    16441 ggtgccggaa gaggaagaaa gcgagaaaaa agagtgaccc attactacaa gaaaggaaat
    16501 cgttatgtcc acattacgcc tgctcatctc tgactcttac gatccgtggt ttaacctggc
    16561 ggtggaggag tgtatttttc gccagatgcc cgccacgcag cgcgttttgt ttctctggcg
    16621 caatgctgac accgtggtga ttggtcgcgc gcagaacccg tggaaagagt gtaatacccg
    16681 gcggatggaa gaagataacg tccgcctggc gcggcgcagt agcggtggcg gcgcggtgtt
    16741 ccacgatctc ggcaatacct gttttacctt catggctggc aagccggaat acgataaaac
    16801 gatctccacc tcgattgtgc tcaatgcact gaacgcgctc ggtgtcagcg ccgaagcgtc
    16861 cggacgtaac gatttggtgg tgaaaaccgc cgaaggcgac cgtaaagtct caggctcggc
    16921 ctatcgcgaa accaaagatc gtggcttcca ccacggcacc ttgctactca atgccgacct
    16981 tagccgcctg gcaaactatc tcaacccgga taaaaagaaa ctggcggcga aaggcattac
    17041 gtcggtacgt tcccgcgtga ccaatctgac cgaattgctg ccgggaatta cccatgagca
    17101 ggtttgcgag gccatatccg aaaccttttt cgcacattat ggcgagcgca cagaagcgga
    17161 aatcatctca ccggacaaaa cgccagactt gccgaacttt accgaaacct ttgcccgcca
    17221 gagtagctgg gaatggaact tcggtcaggc tccggcgttt tcgcatctgc tggatgaacg
    17281 ctttacctgg ggcggcgtag aactgcattt cgacgtcgaa aaaggccata tcacccgcac
    17341 ccaggtgttt accgatagcc tcaaccccgc acctctggaa gctctcgccg gacgactgca
    17401 aggctgccta taccgcgctg acatgctgca acaagagtgt gaagcgctgt tggttgactt
    17461 ccctgaacag gaaaaagagc tacgggagtt gtcggcgtgg atagcgggag cggtacggta
    17521 gttacccgcc catgcgggca actttccctt cgatttgccg gattttttcc ggcatttcct
    17581 gcgcaatgtg gcgaaacgcc tcgctgatac gcggatctcc agcgaccatt tgccagaact
    17641 gctgcgccag cgggatcgcc gttaaccatg cgcttttact gacgttaaga tcaaacttca
    17701 cctcaatggc agcatcacgc agcattccgg cgctgtttgg tgcataggcc atcggcagca
    17761 tgtcgtagac gggcgtcagc gcaaatggtg gttcagataa ataaaatgat aaattgcctg
    17821 cgtgcatatc gctgttggcg ataagtcgcc caaacgccca gatcacttct gtttgcacca
    17881 cgctctggtg agtgacaaat tgttgctcac acaaacggcg cattgcctcc ggccacgatc
    17941 ccggagaaga gataaactca ctctgcaccg cctccagcga cacaatgggc aagcgaccat
    18001 cgttaccttt acagtcaaag cgttgcgctt caaggaatac ctgcctgtta cttgttacaa
    18061 gcaccgttga ctcgatggcg tggatcccac cgtcgcacag gatttgcgcg gcaatagatt
    18121 cagcaattag caggtcaccc caacgttggc tgaccgcggt ttgctgtggc acggtgaatt
    18181 tcaccaacac atgtttattg cctgacggcg tttgtacata gcaggtaaat tttggctgct
    18241 cgccgcccgc agaagaaccc acaatttctc ctgccagtgc atcatgcgcc agttgttcgt
    18301 aacgggtgag tttttgatcc agaggaattg ccgcagggcg tggtgcagta atccatcgct
    18361 gataattcct ctccccgacc agccagcccc cggtatattc accgttaaat acggtcaggg
    18421 cgtagagcac atcttcttcc tgccagagac gtatatcttc agtcagattc agttgtccgg
    18481 ctaaatttct gccccacgcg cgccccaaaa acccctgtgg tcggagatcg gtcaaatacc
    18541 agggcaaacc atcaaaccac tgttcgtcgc cctttccgcc tgtgaccaga caacttccct
    18601 gcggccagca caactggatg tcggcgaatt tatgcgcctt tccggcatcg tccacccgcc
    18661 agacgggaat acgttcaatt ccgcgataag gacgcagcag tgcatatcgc gttgcccgtg
    18721 ctttgccaaa gcgaatcacc cgatcttctc tggcaacaag acgtgagaac gtcgcctgac
    18781 tgattgccag acgctggcgc aattccgggg cagaacgcgg cccttgcagt aaaagatcgg
    18841 tcagttcgct cattaatgaa tagattcgtg aatagatact tgaatagatt tattaacaca
    18901 tttcactgaa aagtatagcg aaaatattca agctggagag aaaaatgaat agatgctaaa
    18961 aagccggagc ggtctcccgc tccggcttca cacgacaatc gcctttcagc gaaacacaat
    19021 tactctttat cgcctagcag aacggattcc agtgcgattt tgatcatgtc gttgaaggta
    19081 gtctgacgct cagcggcagt ggtctgctcg tgcgtgcgga tatggtcaga tacggtgcag
    19141 atggtcaggg ctttcgcgcc gaattctgca gcgacgccgt agataccagc tgcttccatt
    19201 tccacgccga ggatgccgta tttttccatc acgtcgaaca tttcgccgtc cggagagtag
    19261 aacaggtcag cggagaacag gttaccaacg cgagcatcaa tgcccagtgc tttagctgca
    19321 tctaccgcgt tacgcaccat gtcgaagtca gcgatagctg caaagtcatg gtctttaaaa
    19381 cggatgcggt taactttgga atcggtgcag gcacccatac caatcactac gtcacgcagt
    19441 tttacgtgcg gcagaactgc accacaggaa ccaacgcgaa tgattttctt cacgccgaaa
    19501 tcggtgatca gttctttagt gtagatggag caggacggga tacccatacc gtggcccatt
    19561 acagaaattt tgcggccttt gtaagtaccg gtgaaaccca gcataccgcg aacgttgttc
    19621 acttcacggg catcttcaag gaaagtttca gcaatatact tcgcacgcag cgggtcgcct
    19681 ggcatcaaaa ctacgtcagc gaaatcgccc atttctgcat taatgtgtgg agtagccatc
    19741 attttatcct ttttgtgaca tattaaaggc ggagtcactg ctccgcccaa atccatcaga
    19801 acatggcttt gccatattcc atatcagaag taccaaaata ttttgccaga gtctggccga
    19861 tatccgcgaa agtttcacgg tgccccagcg aacccggttt tactttcggg ccgtaaacca
    19921 gtaccggaat gtgttcacgg gtatggtcag taccggtcca ggtcggatca caaccgtggt
    19981 cagcggtgag gatcaggatg tcgtcatcgc gcagcaaaga catcagctct ggcagacggc
    20041 ggtcgaacag ttccagaccc gccgcataac ctgcgacgtc gcgacggtgc ccccaggaag
    20101 agtcgaagtc aacaaagttg gtgaagacga tggtgttatc acccgcttct ttcatctctt
    20161 taatggtggc gtcaaacagc gcgtccaggc cggtcgcttt cacttttttg gtgatgccgc
    20221 agttagcgta gatgtctgca attttaccga cagaaaccac ctggccgtgt ttttcatcaa
    20281 ccagtttctg cagcacggtc ggtgccggcg gttcaacagc caggtcgtga cggttgccag
    20341 tacgctggaa gttaccggct ttatcgccga taaacgggcg agcgataaca cgaccgatgt
    20401 tgtagccgcc gttggtcagc tcttcacggg cgatttcaca cagttcgtag agtttatcca
    20461 gaccgaaagt ttcttcatgg caggcaatct ggaacacgga gtcagcggag gtatagaaaa
    20521 tcggcttgcc ggttttcatg tgctcttcgc ccagctgatc cagaatgacc gtaccggagg
    20581 agtggcagtt accgaggtaa cccggcagat tagcgcgttc gaccagttta tccagtagct
    20641 cttgagggaa gctgttttcg tgatcggaga agtatcccca ctcaaacaga accgggacac
    20701 cggcgatttc ccagtgacca gacggggtgt ctttaccgga tgacatttcg tgcgcccatg
    20761 cgtacgcgcc gataacttca gcgttgccgt ccattcccgc cggaatgaaa ccggtagaac
    20821 cttcgtgtgc tttcgccaac cccagacggg tcagatttgg cagattgagc gggcctttac
    20881 gaccattatc agcttcgcct ttggcacaag cttctgcgat atgacccagg gtgtcagccc
    20941 cgacgtcacc aaagcgttct gcatcttctg tagcgccgat gccgaatgag tccagcacca
    21001 taataaatgc acgtttcata tgttctccgt actcagtgct tcaaatttaa atgatcagat
    21061 cagtataccg ttattcgctg atacggcgat agacagttgg tgtgctttcc ggtgctttat
    21121 cggcaagttt aattgccgct ttcaccgctt tcgccgcttc ctgccagctg ttttcgtctt
    21181 tcgcgtggat aaccgccagc ggacgctgac cgtctacctg gtcacccaaa cgcgccatat
    21241 cagtaaagcc gacgctgtaa tcgatggtgt cagatgcctg acggcgaccg ccgcccattg
    21301 caaccactgc cattcccagc gcgcgggtat ccatatcact gacaaaacct tcggtatcag
    21361 catagactgc tttcgtcagc atcgctgtcg gcagatactt cgcgtagttc tcaacgaagt
    21421 cggtcgggcc tttttgtgcc gctaccatac gaccaaagac ttctgccgct ttaccgttgt
    21481 ccagtaccgc ctgcaatttc gcgcgcgctt cggcgtcatc tttcgccagt ttgccggagg
    21541 taagcatctc cacacacaac gccatcgtga catcaaacag acgcgggtta cggtactcac
    21601 ctgtcaggaa ctgcaccgct tcacgtactt caaccgcgtt accggcactg gaggccagca
    21661 cctgattcat gtcggtgagt agcgcggtgg tgcgcacgcc agcgccgtta gccacgccaa
    21721 caatcgcttc ggcaagggct tcagagagtt cgtaggtcgg cataaatgca ccgctaccga
    21781 ctttcacgtc catcaccagt gcatccagac cttccgccag tttcttcgcc aggatcgagg
    21841 ctgtgatcag cgggatggaa tccacggttg cggtaatatc gcgggtcgca tagaaacgtt
    21901 tatccgccgg agccagtgaa ctggtctggc caataatcgc cacgccgacg tctttaataa
    21961 tttcgcggaa acggttgtca tctgggaaga tgtcgaagcc agggatggat tccagtttgt
    22021 cgagcgtacc accagtatgg ccgaggccgc gaccggagat catcggaata tagccgccgc
    22081 aggcagcgac cattggcccc aacatcagcg aagtcacatc gccgacgccg ccggtcgagt
    22141 gtttatcaac aatcgggcca ttcagatgca ggcttttcca gtcgagaacg gttcctgaat
    22201 ctcgcatcgc catcgtcagc gagacacgct ccggcattgt catatcgtgg aagaaaatgg
    22261 tcatcgcgag ggcggcaatc tgcccttcgg agatagtgtt gtcgcgaatg ccgttgataa
    22321 agaagcgaat ttcttcatcg ctcagcgcat gaccatcacg ttttttacga ataatttctt
    22381 gtgcgagaaa caaggtaacc tcccgtggaa taagtttgaa ggcgttgttg ccagatggcg
    22441 gatgttgtta ttgcctgatg cgatgctgac gcatcataca aggcctacac ggcactcgac
    22501 ccgtaggccg gataaggcgt tccgccgcat ccggcatcca ccctctggca aaaggcagct
    22561 tagtagctgc tggcgctctt accgtcgccg tggcccagag ctttcagcag gcttgccagc
    22621 aggctggaag caccaaagcg gtaatgacgc gcatcagccc agtcagcacc gaacagttca
    22681 tctgcgatgg caagatattt ctgcgcatct tccgcagtac gcacgccgcc cgccggtttg
    22741 aaaccaacgg ttttctctac gcccatatca cggatcactt ccatcatgat gcgcgcgctt
    22801 tccggcgtcg cgttcactgc tactttaccg gtagaggttt tgatgaaatc agcacccgct
    22861 ttgatggata tttcagacgc tttacggatc agcgcttcgt ctttcagttc gccggtttca
    22921 ataatcactt tcagcagtac gttcgctgct gcgcaagcct ctttacaggc tttcaccaga
    22981 tcaaaaccaa cctgctcgtt acccgccatc agtgcacggt acgggaacac cacatcaact
    23041 tcatcggcac cgtaggcgat tgccgcgcga gtttcggcca gcgcgatttc gatatcgtcg
    23101 ttaccgtgtg ggaagttggt taccgtagcg atacggattt ccggggtgcc ctgctctttc
    23161 agcgttttgc gagcaatcgg gataaagcga ggatagatac agatagcggc ggtattaccg
    23221 accggagttt tggcctgatg acacagggcg atcactttct cgtcggtatc gtcgtcattc
    23281 agggtggtca ggtccatcaa tttcagtgca cgcaggctgc ttgctttcag atcagtcatt
    23341 tcattctcct ggcttgtcgc caataaaatt caccttgcga gtttgttagt attctaacat
    23401 cgactccgca acacacttcg atatacatca caattaagga aatctactta caaaattgca
    23461 ttactgtaat gcgatctggt tcaaataatt cactttcaaa tgaatgcgtc agtggtggca
    23521 aacgcatcag gatcaaagtg aacatcacga aacttcttac aatggcgcat caccttcggc
    23581 ataaaaagga acagaagatg tccacttctc atgaaaatgc actgcaacaa cgttgccagc
    23641 aaattgtcac cagcccagtg cttagcccgg agcagaagcg ccattttctg gcactggaag
    23701 cagaaaacaa tctgccttac ccacagctgc ctgccgaagc ccgccgcgcg ctggatgaag
    23761 gtgtaatctg cgatatgttt gaaggtcatg cgccgtacaa accgcgctat gtcttacccg
    23821 attacgcccg ttttctggcg aacggttccg aatggctgga gctggaaggc gcgaaagatc
    23881 ttgatgacgc actctctctg ctgaccattc tttaccacca cgtaccgtcg gtcacatcga
    23941 tgccggtcta cctggggcaa ctggatgcgt tgttgcaacc gtatgttaga attctaacac
    24001 aagacgagat cgatgttcga ataaaacgtt tctggcgtta cctcgacaga accctgccag
    24061 acgcctttat gcacgccaat atcggcccgt ctgattcgcc cattacccgc gcgatcttac
    24121 gtgcagacgc agcgttgaag caggtttcac cgaacctgac ctttatctac gatcctgaaa
    24181 tcacccctga tgacctgctg ctggaagtgg cgaagaacat ctgtgaatgt agcaaaccgc
    24241 acatcgccaa cggtccggtg catgataaaa ttttcacaaa agggggctac gggattgtga
    24301 gctgttacaa ctcactgccg ctggcgggtg gtggcagcac gctggtacgc cttaacctga
    24361 aagccattgc cgagcgcagc gaatcgctgg atgacttctt tacgcgcact ctaccgcact
    24421 actgccagca gcagatcgcc atcatcgatg cgcggtgtga attcctctat caacaatcac
    24481 acttctttga gaatagcttc ctggtgaaag aagggctgat taaccctgaa cgttttgtgc
    24541 caatgtttgg catgtatggg ctggcggaag cggttaactt gctgtgtgag aaagaaggga
    24601 ttgccgcgcg ctacggtaaa gaagccgccg caaatgaagt gggttatcgc atcagcgcgc
    24661 aactggcgga gtttgtcgcc aatacccccg tgaaatatgg ctggcaaaaa cgcgccatgt
    24721 tacacgcaca gtcggggatc agttccgata ttggcaccac gccgggcgcg cgtttgccgt
    24781 atggcgatga gccagatccg atcacccatc tgcaaactgt cgccccgcat catgcttatt
    24841 attattccgg catcagcgac attctgacgc tcgacgaaac catcaaacgt aacccgcagg
    24901 cactggtaca gctttgcctc ggtgccttta aagccggaat gcgtgaattt accgccaatg
    24961 tcagcggtaa cgatctggtt cgcgttaccg gttatatggt acgtttgtcg gacttagaaa
    25021 aatatcgcgc cgaaggttca cgcaccaaca ccacctggct gggcgaagaa gccgcacgca
    25081 acactcgtat tctggaacgc cagccgcgcg tgataagcca tgaacagcag atgcgcttta
    25141 gtcagtaaga ttatcccctt ctcctgcgtt gacgggccag gcagtcgtct ggctctgttt
    25201 ttgcagggct gcaatctgcg ctgcaaaaac tgtcacaatc cgtggacgat gggacgctgc
    25261 aatgattgcg gagagtgcgt gccacagtgt ccgcatcagg cgttgcagat tgttgacggc
    25321 aaagtgctgt ggaacgctgt ggtctgcgag cagtgtgata cctgcctgaa gatgtgtccg
    25381 caacatgcca cgccaatggc gcaatccatg agcgtggacg aagtgcttag ccatgtccgc
    25441 aaagcggtgc tgtttattga agggataacg gtaagcggcg gcgaagccac gacccaactg
    25501 ccgtttgtag tggcgctgtt tactgctatc aaaaacgatc cgcaactgcg ccacctcacc
    25561 tgcctggtgg acagtaacgg tatgttgagc gaaaccggct gggaaaaatt actcccggtg
    25621 tgcgacggcg caatgctcga tctcaaagcg tgggggagcg aatgtcatca gcaattaacc
    25681 ggacgcgata atcagcagat taagcgcagc atctgtttgc tggcagagcg cggtaagctg
    25741 gcggaactgc gtttgctggt aattccagac caggtggatt atttgcatca catcgatgag
    25801 ctggcgaggt ttatcaagag acttggcgat gttccggttc gcctgaatgc gtttcatgcc
    25861 cacggcgtgt atggcgaggc gcaaagctgg gcgagcgcca cgccggaaga cgttgagccg
    25921 ttggctgatg cgttaaaggt gcgcggggtg agccggttga tatttccggc gctctatttg
    25981 tgacatgccg gatacggcct acggttcggc acagacttgt aggcatgata agacgcgaca
    26041 agcgtcgcat caggcattat gcgccgactg ccggatgcgg cgtgaacgcc ttatccggcc
    26101 tacggttcgg cacagacttg taggcatgat aagacgcgtc agcgtcgcat caggcattgt
    26161 gcgccaactg ccggatgcgg cctgaacgcc ttatccggcc tacggttcgg cacagacttg
    26221 taggcatgat aagacgcgtc aagcgtcgca tcaggcattg tgcgctaact gccggatgcg
    26281 gcgtgaacgc cttatccggc ctacggttcg gcacaaacct gtaggcatga taagacgcga
    26341 caagcgtcgc atcaggcatt atgcgccgac tgccggatgc tgcctacgaa gggtaaacta
    26401 cccacgaacg ctaaacaccg catacgtatt attaagcaac acttcggcaa tctcatcttc
    26461 tggttccggg cgcaactcgc aaagcacgtc aaacacccgt gcagcctgtt ccgggcggtt
    26521 aggctgcccc tgaaagccgt tgagcggcat atccggcgcg tcggtttcca gcaacaacga
    26581 tgccagcggt aattttgcga tgacatcgcg ggttttactg gcgcgtggat aggtgatagt
    26641 accgcctacg ccgattttgt agcccaactg tacaaaccgc tcggcctgtt gcaggctgcc
    26701 ggaaaaaccg tgaaccacgc cagtgcgcga taaatcgtgg cgtttaagat gcatcgccaa
    26761 tttgtcgtgt gtgcgccgtg aatgcaggat caccggcaga tcgtagcgtt tcgccagttt
    26821 cagttgttcg tcgagtaacc actgctgcct ctcaaattgc ggatcgtcgc cgaagagatc
    26881 cagaccgatc tcccccaccg ccaccacctt cgccggacgc ctttccagcg cctgctgtag
    26941 ctgatcaaga gacacatcgc tatgtttttc caacatacca ggatgcaagc ccaatgcggc
    27001 atacagcggt tgatattttt ccgctaacgc ctgcacacgg gcaaaatttt ccgcctctgt
    27061 cgccggaaca atgatcttgc ctacgcccgc ttgtgccgcg cgttgcaggc tggcctcttc
    27121 atcgccacta aacggcggga aatcaaaatg gcagtgggtg tcgataaaac ggcaaatcaa
    27181 gccagatcct cattgttaaa tgtcgggtcg ttcgcctgcg gagcgtcaat cacttcggca
    27241 accagcgtat cgttggcgac tggcgcaggc ggaatgacaa tcgattccgg cgtcaccact
    27301 ggcaccagat ggcgggtaag cggcgctttt tcagttaata gcttgccaac cgtggcgagg
    27361 aaataacgac cgcataaacg cccaagttta tagtcttcac gcagcgccgg aatccgactg
    27421 ccaagcgcga tactatgtaa tggcttcggt ggataaattt cgaatatccg cagcttgccc
    27481 ggtggtttct caataaattg ctgaatgtca cgatagctgg tttcatgatg ctgcaccaga
    27541 ttgaccagcg gctgcaggct actgtcaccc agccagcgtt ccatgcgttt gaaccactgc
    27601 ggcgtgtagt acatttgtga cggcacagtg cgaatgacga ccaacgtttt agcaccctgc
    27661 cttgccgcct ctttaaccgg aatcgcatca ctgatcccgc catccaggta gttaatgcct
    27721 tccagcgaca ctccgctacg ataaaagcca ggtatcgcac tggaggcgcg aatcacatcc
    27781 agccagtttt gtttggttgg taaaaagtaa ttcggcgcgt agtcatcctg acgacaggcg
    27841 cacatataaa acgatttgcc gctgtcaaac aaccgcgcgg cggtatccat ttgcaacggc
    27901 atctggcttg cagtggcctc caccagccag tcgagatcga taagatttcc tccacggaca
    27961 aagcgcaatg gatcgaaaaa ttcgcgtttt gtggtatagc gcatgatgac tttgcgcgcg
    28021 taaccgggct gattgcagat aaacgccgag aggttctgcg ccccggcaga tgtgccgaga
    28081 taaagatcga aaggattaaa ctgcgcgcgc ataaactcat ccagcacgcc agccgtgaaa
    28141 attccacgct gtccgccgcc ttcgcacacc agagctattc gtccaggctg gaacggcctt
    28201 agcgacaacg gcgcaatatt accaagcgtt acaggtattc gctgccccac ctctgccttc
    28261 ctgtttttgt tgtgaaagaa tgacaaagta acgcaattta ttattcgcta aaaccacaaa
    28321 agccagtccg ctggactggc tttggataaa attttcttaa tggtctgaaa tctagggtcg
    28381 ttttcggccc atgaacaaac tcaccaggaa cagaataatc ccgacgacaa agacaatttt
    28441 agctgcgcct gcagcggtac cggccagacc accaaaccca agtgcggcgg cgattaacgc
    28501 gataaccaga aatatgatgc cccaacgaaa cataagcgtc tcctttacca tagttaatgt
    28561 caccgcttga tatgagcgtg aaaatcactc aatgcgatat ttttagtgtg gtgcacatcg
    28621 cgcctcccga caaagttcgg gaggacgaat tacgacgaat tacttagttt tcagatcatt
    28681 tttaacgctt ttcacaccat ctaccgcttt ggcgatactt tcagcacggt cactttgtgc
    28741 ctgagaatct acggtaccgg agagctgaac cacaccatcg gtggtttcaa ctttcacttt
    28801 acgtgaagga acgatatcgt ccgccagcag tttggctttg atttcactgg tggtggcggt
    28861 gtcacccgcg tagcccttca cagagccttc tttagcgtcg cgaacgtgca gtttgtcgct
    28921 gacagaggtc acgccttcaa cgcctttggc cactttcact gcctcttcgg cctgagcctg
    28981 gctttcaacg aaaccgctca gggtcacgac tttttgatcg gttttgacag agatatcggt
    29041 gctcttgatg ttgtcatgat ccactagggc cgccttcact ttcgcggtga tggcgctgtc
    29101 atccatgaaa ttaccgactt tattcataga gctatcgact ttttgccctg cgctttcatt
    29161 ggtagtctgc gcgttgtttt ccgcgtaggc agagccggtc gcgacggcag aggtcaacat
    29221 tacagccagc agagttttcg aaatcttcag tcttgtcata gtcatcgatt tattcctgta
    29281 tgtttgctca taatttgagc ctggcaacac gaggttgcat tgctgaatag ggagagactt
    29341 caccctctac agaagtcaat ggccgccatc acaaaagcga tgggtgatga ataacgacca
    29401 ttacagcctc tgaatcagtt attaatatcg gtagaatgac aatcgacggc tttagatact
    29461 gatatctacg cattgaacgg tatttaacgc cgtcagaaat gtcattactt tgttaaatat
    29521 agatcacaat tttgaaaccg ctcgggatat cagctaaaac ataagcaaaa gtgaatgttt
    29581 taagaacatt ccgtaagcgg ctaataagga agggaaattg acagggcgca gcggctaccg
    29641 cgccctggag gcaagaatta atgctcgcgg gtctggtgga actgaacgtc cggataacgt
    29701 tcctgcgcca ggcgcaggtt taccatgctg gtagcgatgt aagcgaggtt atcgccgcca
    29761 tcaagcgcca gttggctttc gttcttacgc ttgaactctt cgaatttctt cgcgtccgca
    29821 cattctaccc agcgggcagt ggcaacgttg actgactcat acactgcttc aacgttgtat
    29881 tcgctcttca ggcgcgatac caccacatca aactgcagca caccaactgc accaacgatc
    29941 aggtcgttgt tggagatcgg acggaacacc tgcaccgcgc cctcttcgga aagctgtacc
    30001 agccctttga gcagctgttt ttgcttcagc ggatctttca ggcggatacg acggaacagt
    30061 tccggcgcga agttcggaat accagtgaac ttcatcatct caccctgggt aaaggtgtcg
    30121 ccgatctgaa tagtgccgtg gttatgcagg ccgaggatat cgcccggata cgcttcttca
    30181 acgtgcgaac ggtcgcccgc cataaaggtc agcgcgtcgg agatcaccac atctttcgca
    30241 gtgcgcacct ggcgcagctt catgcctttt tcatatttac cggataccac gcgcataaac
    30301 gccacgcggt cacggtgttt cgggtccatg ttggcctgaa ttttaaatac gaagccggta
    30361 aatttgtctt cgctcgcttc tacagtacgg gtatcagtct gacgcggcat cggcgcaggt
    30421 gcccactcca ccaggccatc cagcatatga tcaacgccga agttacccag cgcagtaccg
    30481 aagaataccg gagtgatttc gcccgcaagg aacaattctt tgtcgaactc gttagacgcg
    30541 cctttcacca gctccagttc atcacgcaac tgctgcgcca gatcttcacc aaccgcagcg
    30601 tcgagatccg ggttattcag ccctttaaca atgcggactt cctgaatggt gtggccttta
    30661 ccgctctgat agagataggt ttcatcttta taaaggtggt aaacgccttt aaacagcttg
    30721 ccgcagccaa tcggccaggt gatcggtgca cagccgattt tcagctcgtt ctcaacttca
    30781 tcgagcagct ccatcgggtc gcggatatca cggtcaagtt tgttcataaa ggtgaggatc
    30841 ggcgtgtcgc gcagacgggt aacttccatc agcttgcggg tacgatcttc aacacctttt
    30901 gcagcgtcga taaccatcag gcagcagtcc accgccgtca gggtacgata ggtatcttcc
    30961 gagaagtctt cgtgccccgg ggtgtcgagc aggttaacca ggcagtcgtg atacggaaac
    31021 tgcatcacag acgtggtaat ggagatccca cgctgctttt ccatctccat ccagtccgac
    31081 ttagcgtgct ggttagagcc acggcctttt actgtaccgg cggtctgaat ggcctgtccg
    31141 aacagcagca ccttctcggt gatggtagtt ttaccggcgt ccgggtgaga aataatggca
    31201 aaagtgcggc gcttcgccac ctcttgcaaa taaggagaca acgtcataat tctttcttct
    31261 tgagtaagcg gcatcgcgcc gcgcttgttg aaaacgaaaa attgcggcta ttttactcat
    31321 caacgggggg gaggcaatca gtgtttacac aggagctgct ccagttcgtg caacgaagaa
    31381 acggtccagg tgggcgcgat gccttctggt tgctcgcgat tgtgcgcatt cagccagcag
    31441 gtcgcaagcc cggcgttgat gccaccgaga atatcggact cggcagtgtc gccaaccatc
    31501 agcacgcgtg aacggtcagg attgcccgcc tgttccagcg cataatcgaa aattttctta
    31561 ttcggtttag caacgccaac ttcttcggaa atcaccagca aatcgaagta atcacgcagg
    31621 cccgtgcgtt ccagacgcac ctgttgcaag gcgctaaaac cgttggtgat gatgccgatt
    31681 ttggcgttgc cacgaatggc gttaagcaga gaaaccgctc ccggcagcgg cgtacagatt
    31741 tccgccatcg cattaataaa ggcttcgttg agtttacccg gctcgacgtt cagccgttcg
    31801 gcccagctct caaaccgccc gtgctgaagc tgtaatgaag tgatcgcgcc gttttgataa
    31861 tccacccaca gtggcttgtt aacggcctga tagtcctgaa aatcttcagc ggtaaaggtg
    31921 acgctgtaat caagaaacat ccgctgcagg ccggtgaatg agtcaaaggt aaacagcgtt
    31981 tcatcggcat caaagaaaat ccagtcccac ttcattattc caccttgtct tacatactga
    32041 ttggcaacgc catgatgatg gcgtcttcgc gaccgtccgt ggtggggtag taattgcggc
    32101 gaatcgtcgc ctcgttaaag cctaaacttt cgtacagtgc aatggcggca gcgtttgagg
    32161 cacggacttc cagccatagc gtcgccacgc cgcgtttttc cagttcgtcg atcagatgtt
    32221 ccagcagcgc ccttcccagt ccctgacgct gatagtcggg atcgaccgca atattgaaca
    32281 atgtagcttc atccagcacc acttgcgtaa tcgcaaacgc cgccattttg ccgttttgcg
    32341 ttaactgaaa gttgagataa cgctcgccct ggttgctggc aaaggttttt tcactccacg
    32401 gaaaggcgtg ggcgcgttgt tcaatgtgga aagccgctgg taaatcagtc gtttcgaggg
    32461 aagaaatcgt gttcatatgt gcaaatttgt tgccataacg cggcgcgtgc cgttgggttt
    32521 gcccgtaatt cggtgagcgc cggtgatgcc acctgagcgc cttccagtga tagcggttcg
    32581 tcagtaccca accgccagct gttgcagcga ctgccttgcg gcagcatcgc gattttttct
    32641 ggcgtcagtt gcagcacttg atcggggctg acggttaatg cgcgcagaac atcgctcact
    32701 aaaggatcag tcagggcggg aagatcgttt gccaccatca ccagacggac gtgtgccggg
    32761 atcgcaatgg caatctcccc ctgcaacgcg ccagggcgac gcagcgacca ctgggtaatg
    32821 cccagttgct gtaactgcca gtctcgtcgg gatgtcatag cgaatcgctc ctgttgtcag
    32881 gggcgcaaat atagcaaatt cgtcgatacc gcgccaacat atggctataa tcgccgccag
    32941 tatcaattga ggagcattcc atgtctgcat ttaccccggc aagtgaagtc ttgctgcgtc
    33001 acagtgatga tttcgaacaa agccgtattc tttttgccgg agacttacag gatgacctgc
    33061 ccgcgcgttt agataccgcg gccagccgtg ctcataccca gcaattccac cactggcagg
    33121 tgttaagccg ccagatgggt gataacgccc gttttagtct ggtcgccacg gcggatgacg
    33181 tcgcagattg cgatactttg atttactact ggccgaagaa caaaccggaa gcccagttcc
    33241 agttgatgaa tttactttct ctgctgccgg tggggacgga tatttttgtc gttggcgaga
    33301 accgcagcgg cgtgcgcagc gccgaacaga tgctggcaga ttatgcgccg ttgaataaag
    33361 tcgacagcgc ccgtcgctgt ggcctctatt ttggtcgtct ggaaaaacag ccggtatttg
    33421 atgccgataa attctggggc gaatacagcg tcgatggcct gacggtcaaa acgctgcctg
    33481 gcgtgtttag ccgcgacggt ctggatgtcg gtagccagtt gctgctctcg acgttaactc
    33541 cgcacacgaa aggtaaagtg ctggatgtcg gcagcggcgc gggcgtgctt tcggttgcct
    33601 ttgcgcgcca ttcaccgaaa attcgtctca ctctgtgcga tgtctctgcg ccagcggtag
    33661 aagccagccg cgcaacactt gcggccaacg gtgttgaagg tgaagtcttt gccagtaacg
    33721 tcttttccga ggtgaaaggt cgttttgata tgatcatctc caacccgccg ttccacgatg
    33781 ggatgcaaac cagcctcgat gcggcgcaaa cgctgattcg cggcgcggtg cgtcacctta
    33841 acagcggcgg cgagctgcga attgtagcga acgccttcct gccttatccg gacgtgctgg
    33901 atgagacatt tggcttccat gaagtgatcg cgcaaaccgg gcgcttcaag gtgtatcgcg
    33961 ccattatgac ccgccaggcg aagaaaggtt aagtatcccg ccgcaagcct tgtcggatgc
    34021 ggcgtgctcg ccttatacaa cctacaaaac ctgcacgtta gcccttcgta ggccagataa
    34081 gacgcgcccg cgtcgcatct ggcatctgca cccaaatacc gtgtcggatg cggcgtgaac
    34141 gccttatccg acctacaaaa cctgcacgtt agtttcctga tggacatttt tccagcaatt
    34201 acacctccgg cgataattaa ctattgacga aaagctgaaa accactagaa tgcgcctccg
    34261 tggtagcaat tctttttaag aattgatggt atgcgaaggt ggcggaattg gtagacgcgc
    34321 tagcttcagg tgttagtgtc cttacggacg tgggggttca agtccccccc ctcgcaccaa
    34381 ttatctttac ttcctttctt gtttcttccc tgttttgtca tccctgattc aaattcacta
    34441 tcatcagcat gatgccagca ataagcgcca ggcacgatgg tagcaaaatg taatgccgca
    34501 actgtttgtg atacagcatc acaggcgtca atactaatcc catcagaatg acaatccgcc
    34561 actgatccgg cgataaatcc gcatacatca gccctgcaat cagcaatccc ggcagcaaca
    34621 ctccccagcc actgcccagc atacgttgca acatggtttc atctctttca ttgataatga
    34681 taaccaatat catatgataa tttttatcat ttgcaagcca gagaaatccc ttgctatcgg
    34741 gtaaacctat cgctaggatt agcaatcatt atcatttaga ttactatccc gattatggcc
    34801 tatcgttccg caccgctcta tgaagatatc atctggcgaa cgcatctcca gccacaggat
    34861 gctgggcttg cacaagccgt acgtgcgacg atcgccgaac atcgtgaaca tttgctggag
    34921 tttatccgtc tggatgaacc cgccccgctt cgcgccatga cgctggcgca atggtcatca
    34981 ccgaatacgc taagttccct actggcggtc tattccgatc atatctaccg caaccaacca
    35041 acgatgatcc gcgagaacaa gccgctgatc tcactgtggg cacaatggta tatcggcctg
    35101 atggtgccac cattaatgct ggcgctgctg acgcaggaaa aagcattaga tgtgacgccg
    35161 gaacatttcc acgttgagtt tcacgaaacc ggacgcgccg cctgtttctg ggtcgatgtg
    35221 tgtgaagata aaaacgcaac actacattcg ccgcagcagc gaatggaaac gttaatcagc
    35281 caggcgcttg ttccggttgt gcaggcacta gaagcaacgg gagagattaa cggtaaactt
    35341 atctggagca ataccggtta tttgatcaac tggtatctga ctgagatgaa acagctgctc
    35401 ggcgaggcga cggttgaatc gctgcgccat gccctctttt ttgagaaaac gctcacgaac
    35461 ggtgaagata atccactctg gcgtaccgtg gtgctgcgcg acggcctgct ggtgcgccgc
    35521 acttgctgcc agcgttatcg cttaccggat gtgcagcaat gtggcgattg tacgctgaaa
    35581 tgaaagcact gccggggaag taaacccggc atcatgcaaa ttaataggga tgcaacacat
    35641 tacttgcttc gcaatgccgc atacataaca gaatatctgc ggcctccaac aaccctgcat
    35701 cagaactgac gcccagtttc gacatcacat taaatttatg tgcacggatc gttttaatat
    35761 tgcgtttaag ctgctcggca atttgtgtca ttgagtagcc acgcgacata aagcgcaata
    35821 tttctctctc cgtcgggctt agcgtccggc tttggttaat gtaccactga ttgttcagtc
    35881 ggtcggtcgc ctgacgtaca ccatttaatg acaaaaagag ttcctgatga aaaatctcca
    35941 gcgttgacgc tttacttaat acaccgtcca gcggtgatgg cgacaatgaa ccgatcagcc
    36001 gagcttcaat atcatcatcc gcaataacta aacgtcgggt acgcggaaac ttaatcgcca
    36061 gttcagtcag gcaagataat ccctctctgc gttcacttct catggcagaa aaagaaaaaa
    36121 tgaccgccgc aaacgagatc cgcgacatgg catcctgaaa tgactcctga ctggtatagg
    36181 tatggagctt ataagggtta cctgcaaact ttctgaataa actctcaata ccgatactgc
    36241 tcatgacgca tttttctatg agtgcgacat ttctcttctt aattcggctg tgttccattc
    36301 tgcggcatct ccataaacag aaggcaagtt aagttctctt gcaccctgcg cgctatttat
    36361 ccatgcatac atttcagcgt tgctgtgaat tgccagtcga cgcatcgcac tgtttttttg
    36421 ggcgctgata gttttattac ttttcttaag caatgaagct atctggttga tgccccatcc
    36481 tttacctaat agccgcagta ccttgcgctc tgaaagagtg agaatgacgg agcgatagct
    36541 aaaatcatga gtttcagtca ttgccggaga ggtcagcatg gcgctgatac gctctgcgtg
    36601 cgtattgccg gaacgtacgg tcttgaccag attctcaatg ggttcaacat cagacaataa
    36661 cgtcgccgta gggcacatga gcagttctac tgcctgggaa taccaggagc gtgacaccat
    36721 gaaaacccag tgaatttccc gatattgtga gattaaagaa taataatgtt cacaaacaga
    36781 acgtggatct tcactttcac cggctaaatc agcaatgact aatccggaac gacgtaattg
    36841 taataaggta aggtcctccg cagaggcgct ggatattatt tcatattcag ggaagtgagt
    36901 cctcatgacc tcttttaacc ctgcttgcat aacaggtatt ttactgataa caattccatt
    36961 tttgcagcat cctggcaaca tatttcctcc acatccactg tctaaaaatc atgatggttt
    37021 attcaggata ttcattcctt aaatgtttcc agcaaattaa tagattaagc gaataaatta
    37081 tccttagcat tcactttcat atcagcatac tcctgctgca caacaactcc acagcatgtg
    37141 tcagaaggtt agcatcaaat gataa
//
LOCUS       DBANUU010000037        35106 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000037 DBANUU010000000
VERSION     DBANUU010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.037"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(148..1029)
                     /gene="prmA"
                     /locus_tag="ACIE68_001749"
     CDS             complement(148..1029)
                     /gene="prmA"
                     /locus_tag="ACIE68_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFX5908077.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(1041..2492)
                     /gene="panF"
                     /locus_tag="ACIE68_001750"
     CDS             complement(1041..2492)
                     /gene="panF"
                     /locus_tag="ACIE68_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFX5908078.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLVAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2482..2724)
                     /locus_tag="ACIE68_001751"
     CDS             complement(2482..2724)
                     /locus_tag="ACIE68_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFX5908079.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2833..4182)
                     /gene="accC"
                     /locus_tag="ACIE68_001752"
     CDS             complement(2833..4182)
                     /gene="accC"
                     /locus_tag="ACIE68_001752"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFX5908080.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4193..4663)
                     /gene="accB"
                     /locus_tag="ACIE68_001753"
     CDS             complement(4193..4663)
                     /gene="accB"
                     /locus_tag="ACIE68_001753"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFX5908081.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5641..6615)
                     /locus_tag="ACIE68_001754"
     CDS             complement(5641..6615)
                     /locus_tag="ACIE68_001754"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFX5908082.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            6767..8707
                     /gene="csrD"
                     /locus_tag="ACIE68_001755"
     CDS             6767..8707
                     /gene="csrD"
                     /locus_tag="ACIE68_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFX5908083.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            9012..10055
                     /gene="mreB"
                     /locus_tag="ACIE68_001756"
     CDS             9012..10055
                     /gene="mreB"
                     /locus_tag="ACIE68_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFX5908084.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10121..11224
                     /gene="mreC"
                     /locus_tag="ACIE68_001757"
     CDS             10121..11224
                     /gene="mreC"
                     /locus_tag="ACIE68_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFX5908085.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            11224..11712
                     /gene="mreD"
                     /locus_tag="ACIE68_001758"
     CDS             11224..11712
                     /gene="mreD"
                     /locus_tag="ACIE68_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFX5908086.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11721..12314
                     /gene="yhdE"
                     /locus_tag="ACIE68_001759"
     CDS             11721..12314
                     /gene="yhdE"
                     /locus_tag="ACIE68_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFX5908087.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12304..13773
                     /gene="rng"
                     /locus_tag="ACIE68_001760"
     CDS             12304..13773
                     /gene="rng"
                     /locus_tag="ACIE68_001760"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFX5908088.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13841..17641
                     /gene="yhdP"
                     /locus_tag="ACIE68_001761"
     CDS             13841..17641
                     /gene="yhdP"
                     /locus_tag="ACIE68_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFX5908089.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGVRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESIGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17797..19242
                     /gene="tldD"
                     /locus_tag="ACIE68_001762"
     CDS             17797..19242
                     /gene="tldD"
                     /locus_tag="ACIE68_001762"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFX5908090.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19370..20299)
                     /gene="aaeR"
                     /locus_tag="ACIE68_001763"
     CDS             complement(19370..20299)
                     /gene="aaeR"
                     /locus_tag="ACIE68_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFX5908091.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            20482..20685
                     /gene="aaeX"
                     /locus_tag="ACIE68_001764"
     CDS             20482..20685
                     /gene="aaeX"
                     /locus_tag="ACIE68_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFX5908092.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            20693..21625
                     /gene="aaeA"
                     /locus_tag="ACIE68_001765"
     CDS             20693..21625
                     /gene="aaeA"
                     /locus_tag="ACIE68_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFX5908093.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            21631..23598
                     /gene="aaeB"
                     /locus_tag="ACIE68_001766"
     CDS             21631..23598
                     /gene="aaeB"
                     /locus_tag="ACIE68_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFX5908094.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            23690..23962
                     /locus_tag="ACIE68_001767"
     CDS             23690..23962
                     /locus_tag="ACIE68_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFX5908095.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(24018..24281)
                     /gene="yhcN"
                     /locus_tag="ACIE68_001768"
     CDS             complement(24018..24281)
                     /gene="yhcN"
                     /locus_tag="ACIE68_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFX5908096.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(24646..25116)
                     /gene="argR"
                     /locus_tag="ACIE68_001769"
     CDS             complement(24646..25116)
                     /gene="argR"
                     /locus_tag="ACIE68_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFX5908097.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            25551..26489
                     /gene="mdh"
                     /locus_tag="ACIE68_001770"
     CDS             25551..26489
                     /gene="mdh"
                     /locus_tag="ACIE68_001770"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFX5908098.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(26551..27618)
                     /gene="degS"
                     /locus_tag="ACIE68_001771"
     CDS             complement(26551..27618)
                     /gene="degS"
                     /locus_tag="ACIE68_001771"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFX5908099.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(27708..29075)
                     /gene="degQ"
                     /locus_tag="ACIE68_001772"
     CDS             complement(27708..29075)
                     /gene="degQ"
                     /locus_tag="ACIE68_001772"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFX5908100.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(29229..29627)
                     /gene="zapG"
                     /locus_tag="ACIE68_001773"
     CDS             complement(29229..29627)
                     /gene="zapG"
                     /locus_tag="ACIE68_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFX5908101.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            29821..30948
                     /gene="zapE"
                     /locus_tag="ACIE68_001774"
     CDS             29821..30948
                     /gene="zapE"
                     /locus_tag="ACIE68_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFX5908102.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMMRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            31167..31595
                     /gene="rplM"
                     /locus_tag="ACIE68_001775"
     CDS             31167..31595
                     /gene="rplM"
                     /locus_tag="ACIE68_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFX5908103.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            31611..32003
                     /gene="rpsI"
                     /locus_tag="ACIE68_001776"
     CDS             31611..32003
                     /gene="rpsI"
                     /locus_tag="ACIE68_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFX5908104.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            32398..33036
                     /gene="sspA"
                     /locus_tag="ACIE68_001777"
     CDS             32398..33036
                     /gene="sspA"
                     /locus_tag="ACIE68_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFX5908105.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            33042..33539
                     /gene="sspB"
                     /locus_tag="ACIE68_001778"
     CDS             33042..33539
                     /gene="sspB"
                     /locus_tag="ACIE68_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFX5908106.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(33582..34949)
                     /gene="dcuC"
                     /locus_tag="ACIE68_001779"
     CDS             complement(33582..34949)
                     /gene="dcuC"
                     /locus_tag="ACIE68_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFX5908107.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         8482 a         8796 c         9324 g         8504 t
ORIGIN      
        1 cactcaaaga aatttgagta agttctcaat ttttctttat gaatgaaaat tttggcacgc
       61 aaatttgcgt aaataatcgg caaatttacg tgccttttgt gagcttgctc gcacttcgcc
      121 ccgcgtcacc ctacggcgat gcgaaggtta attcttacga ccggtaatgc ggcaccactc
      181 ttctttttcc acgaccgggt ccagtgcgaa gctgtcgaca taagcttcac aaacgctctc
      241 tgcctggctt gccagaatac cggaaaggcc cagcaaaccg cctgaaaccg gcaggacgct
      301 gattaacggt gccagttcac gtaatgggcc tgcaaggatg ttagcgacca ccacgtcggc
      361 tttcatttct tctggctgat ctttcggtaa gtagagttcc agacggtcag aaacgccatt
      421 acgttcggcg ttgtcgcggc tggcctgaat cgcctgcgga tcgatatcaa taccaatggc
      481 ttttgctgca cccagtttca gcgccgcgat cgccagaatg ccggaaccac agccaaagtc
      541 gatgactgtt ttaccggtta aatcgaggct gtcgagccat tgcaggcaca gagaggtggt
      601 tggatgggta cccgtaccaa acgccagccc tggatctaac atcacgttga cggcgttttc
      661 gtccggcaca tcacgccagc tagggcagat ccacagtcgt tcaccaaagc gcatcgggtg
      721 gaaattatcc atccattcgc gctcccagtc tttatcttct agttgttcga ttttatgcgc
      781 gaagcctgcg ccgagcagcg gatggttttc cagaatcgcc accacgtcgt tcatatcggt
      841 ttcagcgtcg aacagaccaa tcacatcggt gtcgccccac aggcgcgttt cgcccggcag
      901 cggctcaaat actggcgtat cgtgggtatc ctgaaaagtg atagaaacgg cacccgcttc
      961 catcagcgca tcgctaagat cttccgcgtt cgcgccggtg gtgttcagtt tcagttggat
     1021 ccaaggcatg gcaaaactct ttatttatca gtagtcaaaa cggtagcttg cgggacggat
     1081 gtaccgaaac ggtttccgac caggaaagcc accaaactta gtagtaacga gggcacgata
     1141 gggtggaagc ccaggtactg aatattcagc gtcgcgagta cggcatacag cacgccgcca
     1201 acgatcatcg cacttagcgc gcctttggcg ttggcgcgtt cccagtaaag acccagcacc
     1261 agcggccaca ggaaaacggc ttccagccca ccgaaggcca gcaaattcag ccagatgatc
     1321 atttctggcg gcttccaggc ggcaagcagc agcaacgcgc cgagaactaa cgtaattacc
     1381 gccgacatcc gcttcagacg cgtctcgttt tgcatttgat ccggacggat attcagatag
     1441 agatctttaa tgatcgtagc ggaactttgt agcaattggg cgttaattgt cgacatgatc
     1501 gcagccatag gtgcagccag aaagatcccg gcagcaaacg gtggcagcac ttttaccatt
     1561 aacgtcggga tcaccagatc cggtacggtg agatcgggga tcactgcacg acctaacgct
     1621 ccggccaggt gcataccgaa catcaggatt gcgactacaa tcgtaccgat gatgatcccc
     1681 cggtgtactg ctttgctgtc tttataagag atacagcgca ccgcagtatg cggcaggcca
     1741 atcacgccaa agcacaccag tacccagaac gacgtcataa aggcaggcga cagaatatcg
     1801 tcagcgcctt gtggcgtaac cagttgcgga tcgatggttt gcaaggtctg tactgcgttg
     1861 cttaagccgc cagcggcatg taccacgcca ataagcagca caacggtgcc aatcagcatc
     1921 acaagccctt gcatggtgtc gttcagcacg ctggcgcgaa agccgccaaa ggcggtatac
     1981 aacgcaatgc tgataccgaa aatcagcagc ccggtttcat aaggaatacc cgccgcggtt
     2041 tccagcaggc gcgcaccgcc gataaactgc acggtcattg ccccaacgaa cgcaaccagc
     2101 aaactcaaac tcgccagcca caccagaaga cgactctggt agcgggcaaa cagcatatcg
     2161 ttcagcgtca ctgcattgta gcggcgcgca agaatcgcga acttcttgcc gagaataccg
     2221 agcgaaagcc agactgcagg aagctgaatc atcgccagca atacccaacc caggccgtat
     2281 ttataagcag ctccaggccc gccgataaac gaactggcac tgatataggt cgcggtgagc
     2341 gtcatcgcca gcacaatacc gcccatagag cggctgccga ggaaatactc attaaggaag
     2401 gtgccggtgc tccgtttacg catcgcataa accgagatac cgaacaccac caccagatag
     2461 gcgaccagcg gtagaattac ttcaagctgc atcgtcatcc tccagtggga tatcgcgata
     2521 gataaatttc accatcgccc agcacagtcc aataaacagt agcggcgtca ggatgcaggc
     2581 catctcaaac cagcgcggaa agccggtaaa accgggggca acgccaggta agtaagcggc
     2641 tactaaccaa actgccagat acaaaagggt cagccccagc gcccagcgcg cctctttatg
     2701 ggcctgaaca aaacgagtgt ccattttttg tccctgatgg gtgaagaaag cgaggattgt
     2761 accttatggg ggttgtcgat ccccagtaat aaaaaaggcc ggaaaatccg gccttttgac
     2821 gcttttgcag tcttattttt cctgaagacc gagttttttc tccagatagt ggatgttagt
     2881 gccaccatgc tggaagttct cgtcattcat gatgcggatc tgcagatcaa cattggtttt
     2941 gataccgtcg atgatcagct cctgcagcgc attcttcatg cgggcaatcg ccacgtcacg
     3001 gttttcaccg tagcaaatca gcttaccgat cattgagtca tagtacggcg gtacggtgta
     3061 gcccgcgtag atatgagact cccaacgtac gccaaaaccg ccaggtgcgt ggaaacgggt
     3121 gattttgcct gggcttggca ggaaggtgtt cggatcttcg gcgttgatac gacattccac
     3181 cgcatggccg cgaacgtgaa cttcttcttg cttgatcgac agcggttgac cggcagcgat
     3241 acgcagctgt tctttgatca ggtcaacgcc ggtgatcatt tcggtaaccg ggtgttctac
     3301 ctgaatacgg gtgttcattt cgatgaaata gaactcgccg ttttcgaaca ggaactcgaa
     3361 agtacctgca ccgcgatagc cgatatcaac acacgcttta gcgcaacgtt cgccgatgta
     3421 gcgacgcagt tccggggtaa tgcccggtgc tggcgcttct tcgaccactt tctggtggcg
     3481 gcgctgcatg gagcagtcac gttccgccag atagatagcg ttgccctgac cgtcagccag
     3541 tacctgaatc tcgacgtggc gaggattttc caggtatttc tccatgtaaa ccatatcgtt
     3601 gctgaaagca gctttcgctt ccgcacgggt catggagatg gattgtgcca gttcagcgtc
     3661 gccgcgcact acgcgcatac cgcgaccgcc gccgccgccg gaggctttga taatcaccgg
     3721 ataaccaatg cgtttagcaa tggcgcggtt tttatccata tcgtcgccca gcgggccgtc
     3781 agaaccaggt acgcaaggaa cgcccgcttt tttcatcgcc gcgattgcgg atactttgtc
     3841 gcccatcagg cgaatggttt ctgctttcgg gccgatgaag ataaagccgg agcgttcaac
     3901 ctgctcggca aagttggcgt tctcggagag gaagccgtaa cccggatgga ttgctactgc
     3961 gccggtgatt tcagcggcgc tgatgattgc cgggatgttc agataacttt ttactgacgg
     4021 agcagggcca atacagaccg tttcatctgc cagtaatacg tgttttagat cgcgatccgc
     4081 gctggagtgc acagcgacag tcttgatgcc cagttcttta caggcacgaa gaatacgcaa
     4141 tgcaatctcg ccgcggttgg caataacaat tttatccagc atgttcgcct cgttactcga
     4201 tgacgaccag cggctcgtca aattctaccg gttgtccact ttcgaccaga attgctttca
     4261 cggtaccgga tttgtccgct tcgatctggt tcatcatttt catggcttca acgatgcaca
     4321 gggtatcgcc cacgttgact ttctgaccca cttcgatgaa cgcttttgcg tccgggcttg
     4381 gggtgcggta gaaagtacca accatcgggg aacgtacgat gtgaccactg atttccgctg
     4441 ctgctggcgc ttccatggaa ggaacggtcg ccggagcggc tgcgttagat tgagctggct
     4501 gctgcatcat tggtgcagcg taagcttgtt gcatcacagg gaaacttgcg gcaggagctg
     4561 cacggctaat gcgtactgac tcttcgcctt cagaaatttc cagttcggag atgcctgatt
     4621 cttcaaccag ctcgatcagt tttttaatct tacgaatatc catgagtggg ttccgtactc
     4681 tttgtttagt gtgattgtga caggcgtttc accgccgtct gtaaagcgta tgccccgaca
     4741 gctgtatgca tagcgataaa ttccagcagg ccgggacgcc ggtctatttt gccccagagt
     4801 gtcaatgtta gacttgacgg acattgtgca gcgatctgct gctatcctcc ggcaaaaaac
     4861 aaaatatacc ttcgttgcgc caatgtcacc ttttccgcag cgcaaaaact gcgtcaggga
     4921 ggacgaggtc gcacattata acgatttcgt agcaattggc agctaaatac tggtcttatc
     4981 agggaagata atcaacagct aacatgtaaa taaccttcaa caccgtgtaa tttgcaacaa
     5041 gccgcacaat tcacgaaatt agcgccatca tcgacggaac ttcttataac gtaaggtgaa
     5101 aaactgtaaa gccagccctg tgaagatgag gggctgcaat gagataatct tcacagacca
     5161 caaataatgt agggatgcca ggatcgcgac aagatagacg acattacgca gcggttgctg
     5221 aggattaccc aattttcact gcatcgtctg gatagacgtg aatgctaaag cacgcaaaat
     5281 caaccgactg acaatgctca gcattaaata ggtttgagtg gttaatttgt agtcaaataa
     5341 aatcagatta ttcaccccat ggtaccagaa ataagtaagg ggctaagcgt agcgtcgccc
     5401 aggcaaagca ccataaacct aacaggcgac ggaggcgtat caataatagc tgtttagtgg
     5461 agtatcaatg cgcatcaatt tgtggtcagt cgaaatcatg actttacagt ttggatgatg
     5521 ttgtataact ttctgaccaa ggtgttcgac gaaagctgtc cggctaaccg cttttcccgt
     5581 taaccgattg aataatgatt tcaaaatgag ttccctctct tattattccc tgctaaatgg
     5641 ttagttaacc ttcaccagcg tgcgaccctg gatctggtta ttaatgatgg cctcggcaaa
     5701 gttcggtgcc tctgacagag atatctcttt tgccgcctgg gtatagaatg attccggtaa
     5761 atcggcgacc agtcgctgcc aggcttgtgc gcggcgttct ggtggcgtca ttactgaatc
     5821 caccccttgc aaacggacat tacgcagaat aaatggcatg accgtggttg gcagagtaaa
     5881 accacccgcc agaccacagg cagccacgca gccgccgtaa ttcatttgcg ccagcacttt
     5941 tgccagcact ttgtcgccaa cagtgtcaat tgccccagcc cagacttgtt tttccagagg
     6001 acgggattcg gcaaactcat cacgagggag aatacgactg gcacctaaac ttttcagata
     6061 ttcatgggta ctttcgcgac cggaaacggc aacgacctga taacccaact tatgcagcag
     6121 cgccacggcg gtactgccga cgccaccact ggcacccgtc acgacaatct ccccgtcctg
     6181 cgggcgaaca ccggcatctt ccagcgccat cacacacagc atggcggtaa aaccggcagt
     6241 accgataatc attgctttac gcgcgtccag cccttgcggc atggcaacca gccagtcacc
     6301 tttcactcgc gcctgctccg ccagcccacc ccagtggttt tcaccaacgc cccagccagt
     6361 gagtaacacc tcctgaccgg catgaaaacg cggatcttcg ctggtgcgta cagttccggc
     6421 aaaatcgatc ccaggaatca tcggaaaatt acggatgatt tttcccttac cggtaatcgc
     6481 cagcgcatct ttatagttca ggctcgacca gtgaacatca accgtgacat cgccctccgg
     6541 caggcgactt tcgtcaagtg tctgtaccga tgcgagagtt ttgccgtcct gctgttctaa
     6601 aagtaacgcc tgcataagtg gtcctcatgt gcatgatgga ttggaaaata atttctgaag
     6661 actatactcg ctaatggaaa agcaaagccg atgaagcgca ataaattgcg agataaatct
     6721 gatttgctag tatgcccgct tcctcactat cggagttaac acaaggatga gattaacgac
     6781 gaaattttcg gcctttgtta cgctgctcac cgggttaaca atttttgtga ctttgctggg
     6841 ctgttcgcta agtttctaca acgcaattca gtataagttt agtcatcgcg ttcaggcggt
     6901 ggcgacggcg attgataccc accttgtgtc gaatgacttc agcacattaa ggccacaaat
     6961 taccgaatta atgatgtcgg cagatatcgt tcgtgtagac ctgctccatg gtgataaaca
     7021 ggtttatacc ctggccagaa atggtagtta tcgtccggtt ggctccagcg atctgtttcg
     7081 cgaactgagt gttccgttga taaagcatcc ggggatgtcg ctgcgtctgg tttatcagga
     7141 tccgatgggc aactatttcc attctttaat gactaccgcg ccgctcacgg gggcgattgg
     7201 ctttatcatt gttatgctct tcctggcggt acgctggtta caacggcaac ttgccgggca
     7261 agaattgctg gaaacccggg ctactcgtat cttaaacggt gagcgtggct ctaatgtgtt
     7321 gggaaccatc tatgaatggc cgcccagaac cagcagtgcg ctagatacgc tgcttcgtga
     7381 aattcagaac gcacgcgaac aacacagccg tcttgatacg ctgatccgct cttatgccgc
     7441 ccaggacgtg aaaaccggcc tcaataaccg actctttttc gataatcagt tagcaacgtt
     7501 actggaagat caggagaaag taggtaccca cgggatcgtg atgatgattc gtctgccgga
     7561 tttcaatatg ttgagcgata cttgggggca cagccaggtt gaagaacagt tcttcactct
     7621 gacgaatctg ctgtcgacat ttatgatgcg ctaccctggc gcactgctgg cgcgttacca
     7681 ccgcagtgat tttgctgcgc tgttaccgca ccggacgtta aaagaggcag agagcatcgc
     7741 cggtcagtta atcaaagccg tcgatacctt gccgaacaat aaaatgctcg atcgcgacga
     7801 tatgatccac attggtatct gcgcctggcg tagtggtcag gataccgagc aggtaatgga
     7861 acatgcagag tctgccacgc gtaatgcggg attgcagggc ggcaatagct gggctattta
     7921 cgatgactcg ttgcctgaaa aaggacgcgg taatgttcgc tggcgtacgc ttatcgagca
     7981 aatgctcagt cgcggcggcc cgcgccttta tcaaaaaccg gcggttactc gcgaaggtca
     8041 ggttcatcat cgcgaactca tgtgccgcat cttcgatggt aatgaagagg ttagctcggc
     8101 ggagtatatg ccgatggtct tgcagtttgg cttatcggaa gagtatgacc gtctgcaaat
     8161 cagccgtctt attccactat tgcgttactg gccagaggaa aatctggcga ttcaggttac
     8221 cgttgagtcg ctgattcgcc cgcgttttca gcgttggctg cgcgatacgt taatgcaatg
     8281 tgaaaaatca caacgaaaac gcataattat tgaacttgca gaggccgatg taggtcaaca
     8341 tatcagtcgt ttacaacctg ttattcgttt agtaaatgct ttaggggtac gggtagccgt
     8401 caaccaggct ggtttgacgc tggtaagcac cagttggatc aaagaactta atgttgagtt
     8461 actcaagctc catccggggc tggtcagaaa cattgagaag cgaacggaga accaactgct
     8521 ggttcaaagc ctggtggaag cctgctccgg gaccagcacc caggtttacg ccaccggtgt
     8581 gcgttcgcga agcgagtggc agaccctgat tcagcgcggt gttacaggcg ggcaagggga
     8641 ttttttcgcg tcctcacagc cacttgatac taacgtgaaa aaatattcac aaagatactc
     8701 ggtttaacct gccgtttaat ccgttttcac gtagaataac gcgcgctgcg tctcatggga
     8761 gtgtgcttgt ctgctcgcca gattgttgca gcacatatgc agatgaatga ccttacgcgg
     8821 ttgcaaacag gcgaggaatg ctgctgatgc attaagcctt tctggactca ggcagagatt
     8881 tgtaacaaag gaaacgaact gcactaattt tcaccgtagc agatgatttt tgcgccttgt
     8941 cgctgctgcg tgtggttggt aaagtaagcg gattttcttt tccgccccag ctttcaggat
     9001 tatcccttag tatgttgaaa aaatttcgtg gcatgttttc caatgacttg tccattgacc
     9061 tgggtactgc gaataccctc atttatgtaa aaggacaagg catcgtattg aatgagcctt
     9121 ccgtggtggc cattcgtcag gatcgtgccg gttcaccgaa aagcgtagct gcagtaggtc
     9181 atgacgcgaa gcagatgctg ggccgtacgc cgggcaatat tgctgccatt cgcccaatga
     9241 aagacggcgt tatcgccgac ttcttcgtga ctgaaaaaat gctccagcac ttcatcaaac
     9301 aagtgcacag caacagcttt atgcgtccaa gcccgcgcgt tctggtttgt gtaccggttg
     9361 gcgcgaccca ggttgaacgc cgcgcaattc gtgaatccgc gcagggcgct ggtgcccgtg
     9421 aagtcttcct gattgaagaa ccgatggctg ccgcaattgg tgctggcctg ccggtttctg
     9481 aagcgaccgg ttctatggtg gttgatatcg gtggtggtac cactgaagtt gctgttatct
     9541 ccttgaacgg cgtggtttac tcctcttccg tgcgcattgg tggtgatcgt ttcgacgaag
     9601 ctatcatcaa ctatgtgcgt cgtaattacg gttctctgat cggtgaagcc accgcagaac
     9661 gtatcaagca cgaaatcggt tcggcttacc cgggcgatga agtccgtgaa atcgaagttc
     9721 gtggccgtaa cctggcagaa ggtgttccac gcggttttac cctgaactcc aacgaaatcc
     9781 tcgaagcact gcaggaaccg ctgaccggta ttgtgagcgc ggtaatggtt gcactggaac
     9841 agtgcccgcc ggaactggct tccgacatct ccgagcgcgg catggtgctc accggtggtg
     9901 gcgcactgct gcgtaacctt gaccgtttgt taatggaaga aaccggcatt ccagtcgttg
     9961 ttgctgaaga cccgctgacc tgtgtggcgc gcggtggcgg caaagcgctg gaaatgatcg
    10021 acatgcacgg cggcgacctg ttcagcgaag agtaatcgga tgcaggcagg ggaaatgtct
    10081 gtttaccctg cctggtctga tacgagaata cgcataactt atgaagccaa tttttagccg
    10141 tggcccgtcg ctacagattc gccttattct ggcggtgctg gtggcgctcg gcattattat
    10201 tgccgacagc cgcctgggga cgttcagtca aatccgtact tatatggata ccgccgtcag
    10261 tcctttctac tttgtttcca atgctcctcg tgaattgctg gatggcgtat cgcagacgct
    10321 ggcctcgcgt gaccaactag aacttgaaaa ccgggcgtta cgtcaggaac tgttgctgaa
    10381 aaacagtgaa ctgctgatgc ttggacaata caaacaggag aacgcgcgtc tgcgcgagct
    10441 gctgggttcc ccgctgcgtc aggatgagca gaaaatggtg actcaggtta tctccacggt
    10501 taacgatcct tatagcgatc aagttgttat cgataaaggt agcgttaatg gcgtttatga
    10561 aggccagccg gtcatcagcg acaaaggtgt tgttggtcag gtggtggccg tcgctaaact
    10621 gaccagtcgc gtgctgctga tttgtgatgc gacccacgcg ctgccaatcc aggtgctgcg
    10681 caacgatatc cgcgtaattg cagccggtaa cggttgtacg gatgatttgc agcttgagca
    10741 tctgccagcg aatacggata ttcgtgttgg tgatgtgctg gtgacctccg gtctgggcgg
    10801 tcgtttcccg gaaggctatc cggtcgcggt tgtctcttcc gtaaaactcg atacccagcg
    10861 cgcttatact gtgattcagg cgcgtccgac tgcaggactg caacgtttgc gttatctgct
    10921 gctgctgtgg ggcgcggatc gtaacggcgc taacccgatg acgccggaag aggtgcatcg
    10981 tgttgctaat gaacgtctga tgcagatgat gccgcaggta ttgccttcgc cagacgcgat
    11041 ggggccaaag ttacctgaac cggcaacggg gatcgctcag ccgactccgc agcaaccgac
    11101 gacaggaaat gcagctactg cgcctgctgc gcccacacag cctgctgcta atcgctctcc
    11161 acaaagggct acgccgccgc aaagtggtgc tcaaccgcct gcgcgtgcgc cgggagggca
    11221 atagtggcga gctatcgtag ccagggacgt tgggtaatct ggctctcttt cctcattgcg
    11281 ctgttgctgc aaatcatgcc ctggccggat aacctgattg ttttccggcc aaactgggtg
    11341 ttactcatct tgttgtattg gatcctggcc ttgcctcatc gcgtaaatgt gggcacaggt
    11401 tttgtgatgg gtgccatact ggatctgatc agcggctcga cgcttggcgt acgcgtattg
    11461 gcgatgagca tcattgctta cctggtggcg ctgaaatacc agcttttccg caacctcgca
    11521 ttatggcagc aggcgctggt cgtcatgttg ctttcgctgg tggtggatat tattgttttc
    11581 tgggcagagt ttttagtgat taacgtctct ttcagaccgg aagtgttctg gagtagtgta
    11641 gtcaatgggg tgctctggcc gtggattttc ttgctgatgc gtaaagtccg tcagcagttt
    11701 gcagtgcaat aaaggtttct atgacttctc tgtatttagc ttccggttct ccgcgtcgtc
    11761 aggagttact tgcgcaactt ggcgtgacct ttgaacgtat tgttacgggc attgaggagc
    11821 agcgtcagcc gcaggagagc gcgcagcagt atgttgtgcg tctggcgcgc gagaaagcac
    11881 aggcgggtgt cgcgcaaacg gcgcaggatc tcccggtgct gggtgcggat actatcgtta
    11941 tcctgaatgg agaagtgctg gagaaaccgc gcgacgcaga gcatgcggcg cagatgttgc
    12001 gcaaattatc gggtcagacc catcaggtga tgacggcagt ggcgttggcc gacagccagc
    12061 acattctcga ttgcctggtg gtcaccgatg tgactttcag aacgttaaca gacgaagaca
    12121 tcgcgggcta tgtcgccagc ggtgaaccgt tagataaagc aggtgcatac ggtattcagg
    12181 ggctgggtgg ctgttttgtc aggaagataa atggcagcta tcacgccgta gtcggcttac
    12241 cgctggttga aacgtatgaa ttattaagta attttaacgc actgcgtgag aaaagggata
    12301 aacatgacgg ctgaattgtt agtaaacgta acgccttcgg aaacgcgagt ggcgtatatt
    12361 gatggcggta ttctgcagga aattcatatt gaacgtgagg cgcgacgcgg aatagtaggc
    12421 aatatctaca agggtcgtgt aagtcgtgta cttccgggta tgcaggcggc ttttgtagat
    12481 attgggctgg ataaagccgc gtttcttcat gcatccgata tcatgccgca caccgaatgt
    12541 gtggcgggtg aagaacaaaa gcaattcacg gtgcgcgaca tctcggaact ggttcgtcag
    12601 gggcaagatc tgatggtgca ggtggtgaaa gatccgcttg gcactaaagg tgcgcgcctg
    12661 accaccgata tcacgctgcc ttctcgctat ctggtgttta tgccaggggc ttctcacgtt
    12721 ggggtttccc aacgtattga aagcgaatca gaacgtgaac gcctgaaaaa agtggtcgca
    12781 gagtattgcg acgagcaggg cgggtttatc atccgtaccg cagcggaagg ggttggcgag
    12841 gctgaactgg cctccgatgc cgcttatctg aaacgcgtct ggaccaaagt tatggagcgt
    12901 aaaaaacgcc cgcagacccg ttatcagctg tacggcgaac tggcgctggc gcagcgtgtt
    12961 ctgcgtgatt tcgccgacgc cgaactggac cgcattcgcg ttgactcacg cctgacttac
    13021 gaagcattgc tggagtttac ctcggagtac attcccgaga tgaccagcaa gctggagcat
    13081 tacaccggac gccagccgat tttcgatctc tttgatgtcg aaaacgaaat ccagcgagcg
    13141 ctggaacgca aagtagaact gaaatccggt ggttatctga ttatcgacca gaccgaagcg
    13201 atgaccaccg tggacatcaa taccggagcg tttgtcggtc atcgcaatct tgacgacacc
    13261 attttcaata ccaatattga agcgacgcag gctatcgctc gccagttacg gttgcgcaat
    13321 ctgggcggga ttatcattat tgatttcatc gatatgaata atgaagatca ccgccgccga
    13381 gtgctgcact cgctggagca ggcgttgagc aaagaccggg tgaaaaccag cgttaatggt
    13441 ttttcggcgc tggggctggt ggagatgacg cgtaaacgca cccgcgaaag cattgagcac
    13501 gtactgtgta acgaatgccc aacctgccac ggtcgcggaa cggtgaaaac cgtggaaacg
    13561 gtatgctatg aaatcatgcg cgagattgtt cgtgtccacc atgcttacga ctccgaccgt
    13621 ttcctggtct atgcttctcc ggcagtagct gaagccttga aaggcgaaga gtcacactcg
    13681 ctggcggaag tggaaatttt cgttggcaaa caggttaaag tacaaattga accgctctat
    13741 aaccaggagc agtttgacgt cgtaatgatg taaacagatg ctgggccgcc atccggcaaa
    13801 gggtttttga gtcacatttt tagcagacaa ggagtgacgg gtgaggcgat tgccggggat
    13861 tttactgctt actggagccg cgctcgttgt gatcgctgcc ctgctggtta gcggcctgcg
    13921 tattgcttta ccgcatcttg acgcctggcg tccggaaatc ctcaacaaaa tagaatccgc
    13981 gactggcatg ccggtagaag ccagtcagct ctcagccagc tggcagaatt ttggcccgac
    14041 gcttgaagca cacgacatcc gtgcagaact aaaagatggc ggcgaatttt cggttaaacg
    14101 cgttactctg gcgctggatg tctggcagag cttgttacat atgcgctggc agtttcgcga
    14161 cctcactttc tggcaactgc gctttcgcac caacactcct atcaccagcg gtggtggtaa
    14221 tgatagcctg gaagccagtc acatcagcga tctgtttctt cgtcaatttg accatttcga
    14281 tctccgcgac agtgaagtca gttttctgac gccatccggt cagcgcgccg agctggcgat
    14341 cccacaactc acctggctga acgatccacg tcgacaccgt gcggaaggcc tggtaagcct
    14401 ctccagcctt accggacagc acggcgtgat gcaggtgcgt atggatttgc gcgatgatga
    14461 ggggttgtta agcaatggtc gcgtctggct acaggcggat gacatcgacc tgaagccgtg
    14521 gctcggtaaa tggatgcagg acaatattgc tctggaaacg gcacagttct cccttgaagg
    14581 ctggatgacg atcgacaaag gcgatgtaac cggcggtgac gtctggctga aacagggcgg
    14641 tgccagctgg ttgggcgaga agcaaacgca tacgctgtcg gtggataatc tgaccgcgca
    14701 tattacgcgt gaaaatccgg gctggcagtt ctctattccc gatacacgga tcacgatgga
    14761 cggcaaaccc tggccgagcg gagcattgac gctggcctgg ataccggaac aggacgttgg
    14821 cggcaaagac aataaacgca gtgacgaact ccggattcgc gccagtaatc tggagctggc
    14881 aggcctggag ggcgtacgcc cgctggccgc gaaactttca cctgcactgg gtgatgtttg
    14941 gcgctccaca caaccgagcg gcaagattaa cactctggcg ctggatatcc cgcttcaggc
    15001 ggcagacaag acccgttttc aggcatcgtg gagcgatctg gcctggaagc aatggaaatt
    15061 attaccgggt gcggaacact tctccgggac gctttccggc agcgttgaaa atggtttgct
    15121 taccgcgtcg atgaagcagg caaagatgcc ttacgaaacg gtattccgtg cgccactgga
    15181 aatcgccgac ggccaggcaa ctataagctg gctgaacaat gacaaaggtt tccagctgga
    15241 tgggcgtaat attgacgtta aagccaaagc cgtccatgcg cgcggcggtt ttcgttacct
    15301 gcaacctgct aacgatgaac cctggctggg tattctggct ggcatcagta ccgatgatgg
    15361 ttcacaagcc tggcgctatt tcccggaaaa cttgatgggt aaagacctgg ttgattattt
    15421 aagtggtgcg attcagggcg gtgaagcgga taacgcgacg ctggtttatg gtggcaatcc
    15481 gcaactcttc ccctataaac acaacgaagg tcagtttgaa gtgctggtgc cgctgcgcaa
    15541 cgcgaagttt gccttccagc cggactggcc tgcattaact aaccttgata ttgaactgga
    15601 ctttattaac gacggtttat ggatgaaaac cgatggcgtt aatctgggcg gcgtgcgcgc
    15661 gagtaatctc accgcagtga tccctgacta ctcaaaagaa aaactgctga ttgacgctga
    15721 cattaaaggt ccgggtaaag ccgtaggccc ttactttgat gagacaccgc tgaaagattc
    15781 cctgggtgcg accctgcaag aactccagct cgacggcgat gtgaatgctc gcttacatct
    15841 tgatatcccg ctgaacggcg agctggtaac cgcgaaaggt gaagtgacgc tgcgtaataa
    15901 cagtctgttt atcaaaccac tcgacagcac cctgaaaaat ttgagcggta aattcagctt
    15961 tatcaatggc gatctgcaaa gtgaaccact gacagcaagc tggtttaatc agccgttgaa
    16021 cgtggatttt tccaccaaag aaggggcaaa agcctaccag gttgcggtga atctcaacgg
    16081 taactggcaa ccggcgaaaa ccggcgtttt gcctgaagcg gtgaacgaag cattaagtgg
    16141 cagcgtggcg tgggatggta aagtgggcat tgatctgcct tatcatgctg gcgcgaccta
    16201 taacgtagag ctgaacggcg atctgaagaa tgtgagtagt cacttacctt caccgttagc
    16261 caaacctgcg ggtgaaccac tagcggtaaa cgttaaggtt gatggcaatc tcaacagctt
    16321 tgaattaacc ggacaggctg gtgcggataa ccatttcaat agccgctggt tgcttggtca
    16381 aaagctgacg ctcgatcgtg ctatttgggc ggcagacagt aaaacgctcc cgccgttgcc
    16441 ggaacaaagt ggcgttgaac tcaatatgcc gccgatgaat ggtgccgagt ggctggccct
    16501 gttccagaaa ggcgctgcgg agagtatcgg tggtgcagcg agtttcccac aacacataac
    16561 gttacgtacg cctatgttgt cactgggaaa tcagcaatgg aataacctga gtattgtttc
    16621 gcaaccgacg gcaaatggca ccctggttga ggcgcaaggg cgtgaaatca acgccacgct
    16681 agcgatgcgt aataacgcgc cgtggctggc gaatatcaaa tatctttatt acaacccgag
    16741 cgtggcgaaa actcgtggtg attcaacgcc gtcatcacct ttcccgacaa cggagcgcat
    16801 taacttccgt ggctggccgg acgcccaaat acgatgcaca gagtgctggt tctgggggca
    16861 aaaattcggt cgcattgaca gtgatatcac catttctggc gatacgttaa cgctgaccaa
    16921 tggactgatt gatactggtt tctcgcggct tactgccgat ggtgaatggg ttaataatcc
    16981 ggggaatgaa cgtacctcgc tgaaaggaaa actgcgcggg cagaaaattg atgccgccgc
    17041 agaatttttt ggtgtcacga cgcccatacg ccagtcgtca tttaatgtgg attacgattt
    17101 acactggcgc aaagcaccct ggcagccaga tgaagcgacg ttgaatggca tcattcatac
    17161 tcaactgggt aaaggcgaaa ttaccgaaat caataccgga catgccgggc aattgctgcg
    17221 cttattgagc gtagatgccc tgatgcgtaa gctgcgtttt gatttcagag acacttttgg
    17281 cgaagggttc tattttgact ccattcgcag caccgcgtgg attaaagacg gcgttatgca
    17341 caccgacgac acgctggtgg atggcctgga ggcggatatc gccatgaaag ggtcggtaaa
    17401 tctggtacgt cgcgacctga atatggaagc ggttgtcgca ccagagattt ctgcgacggt
    17461 gggcgtggct gcggcttttg cggttaaccc cattgttggc gcggcagtgt ttgccgccag
    17521 taaagtgctg gggccgctgt ggagcaaagt ctccattttg cgctatcaca tttcgggtcc
    17581 gctggacgat ccgcaaatca acgaagtgtt gcgccaaccg cgtaaagaaa aagcgcaatg
    17641 atttgacgag ggcgcgtaat tgccccaatc tcatatgata atcgttgcca aaggccaacg
    17701 agccagaaca taaccgtagg tcggataggg cgttcacgcc gcatccggca gccgtaaaaa
    17761 atcctctact gcagtaacta acgagtagca aaaacgatga gtcttaacct ggtaagtgaa
    17821 caattgctag cggcgaacgg cctgaaacat caggacttgt tcgcgatcct cggtcaactg
    17881 gccgaacgtc gccttgatta tggcgatctc tattttcagt cgagctatca cgaatcctgg
    17941 gttttagaag accgcattat taaagatggt tcttacaaca tcgatcaggg cgttggtgtg
    18001 cgtgcaatca gtggtgaaaa aaccggattt gcttacgctg accaaatcag cctgctggcg
    18061 ctggaacaga gtgcgcaagc ggcgcgcacc atcgtccgtg atagtggtga tggcaaagta
    18121 cagacgctgg gcgcggtaga gcatagcccg ttgtatacct cggtagatcc gctgcaaagc
    18181 atgagccgtg aagagaagct ggatatcctg cgtcgcgtcg ataaggttgc ccgcgaagcg
    18241 gacaagcgcg tacaagaagt gactgccagc ctcagcggcg tttatgaatt aattctggtt
    18301 gcggccaccg acggcacgct ggcggcggat gttcgtccgc tggtgcgtct ttccgtgagc
    18361 gttctggtcg aagaagatgg caaacgcgaa cgcggtgcca gtggcggcgg cggtcgtttt
    18421 ggttatgagt tcttccttgc cgatcttgac ggcgaagtcc gtgcggatgc atgggcaaaa
    18481 gaagcagtgc gtatggcgct ggtcaatctt tctgccgttg ctgcaccagc gggcaccatg
    18541 ccggtagtac ttggcgcagg ttggccgggc gtgctgttgc atgaagcggt aggtcacggt
    18601 ctggaaggcg acttcaaccg ccgcggcact tcagtattta gtgggcaggt cggggagctg
    18661 gtggcttcag aactgtgtac cgtggttgat gacggcacga tggtcgatcg ccgtggttcg
    18721 gtggcgattg atgacgaagg tacgccaggc cagtacaacg tgctgattga gaacggcatt
    18781 ctgaaaggct acatgcagga taaactcaac gcgcgtttga tggggatgac gccgactggc
    18841 aacggtcgcc gtgaatccta cgcccatctg cccatgccgc gtatgaccaa cacctatatg
    18901 ctgccgggta aatcgacccc gcaggaaatt attgaatccg ttgagtacgg tatctatgca
    18961 ccgaactttg gtggcggtca ggtggatatt acatccggca aattcgtttt ctccacttca
    19021 gaagcgtatc tgattgaaaa cggtaaagta acgaagccgg tgaaaggcgc gacgttgatt
    19081 ggttccggta tcgaaaccat gcagcagatt tcgatggttg gcaacgacct gaaactggat
    19141 aacggcgtgg gtgtctgcgg taaagaaggg caaagtttgc cggttggcgt gggccagcca
    19201 acgctgaaag ttgataacct gactgttggc ggtactgcgt aataatttca ttattttcag
    19261 aaggataatt aatctttcta cgtgcacgaa cggtcccctc gctcctctgg ggttagggtg
    19321 aggggaaccc gttggcacag gtttgtagaa cgtaacagta caatatgaat tacttctctt
    19381 tcccgcgccc gtgcatctcc tgaaacaatt taccgacctc aacaaaataa tccgtcagcg
    19441 agttgatcac gacctgtacc ttcagcggca gcttatcttt ttcggtatat aacgcataaa
    19501 ccgggcgtgg atctgactgg taacgtggta gcaggatctc cagctccccg cgattgatct
    19561 cgttgatcac ccacatcagc ggcacgtagg cgatcccagc gcctgcagtc agccagcgca
    19621 ccagcgtcat cggatcatta gtcacaaatc tcccctgcgg gatcaggcga gtcgagatcc
    19681 cttccggtgc gatcagttca aattcattgt cgggccgcac gctgtattca agccatgaat
    19741 ggctacttaa atcggcgggt ttttccggta tgccgtattg tgtgagatag ctttttgcgg
    19801 cgcacaccac cattggcatc gcgcccagac ggcgggaaaa caggctggaa tcctgcaacg
    19861 cgccgacgcg gatcaccaca tccagaccgt cggcaatcag gtcgggggca ggaattccgg
    19921 taaccagatt gacgctcaaa cctgggtatt ctttcagcat ttttgctgtc agcccggcga
    19981 gaacattttg tgccatagtt gaagaacagc caatgcgcag cgtcccgatg ggggtgttat
    20041 tgaaggcata cagttgctca tgaacatcct gcacttcatg aagcatacga cggcagccct
    20101 ggtagtaaat tctaccggct tcggtcaggc caatgctgcg tgtgctacgg tttaacagct
    20161 ttacctgcaa ctcatcttcc agttttgaca ccgtctgact gatggacgaa acgctcatct
    20221 gtagctgtct ggcggcggcg gtaaaagagc caaattcaac tactttggca aacaccgaca
    20281 tgcgttttag tcgttccatt attcactctg acttaaaagt gatttagatc acataatata
    20341 gataacagca taacagttac gctaatatat taaatatcaa tctacagcaa tgttgctctc
    20401 gcccggcttt ccatgccctt ctctgcggcg acagatgctg aaaataataa cgcctgctct
    20461 ctctttacaa ccaaggtcaa catgagtctg tttcccgtta tcgtggtgtt tgggctgtcc
    20521 ttcccaccga tattttttga attgctttta tcactggcga ttttctggct ggtgcgccgg
    20581 gtacttgtgc caacaggtat ctacgacttt gtctggcatc cggcgttgtt caacaccgcg
    20641 ctctattgct gcttgtttta tttgatatcg cgactgttcg tttgaggttg aagtgaaaac
    20701 actaataaga aaattctccc gtacggccat cacggtcgta ttagtcattc tggccttcat
    20761 cgcaattttt aatgcctggg tctattacac cgaatccccc tggacgcgtg acgcgcgctt
    20821 tagcgctgac gtcgttgcga tcgcgccgga cgtttctgga ctcattaccc aggtgaatgt
    20881 tcatgataac cagctggtga aaaaaggaca gatactgttc accatcgacc agccgcgcta
    20941 tcaaaaggcg cttgaggaag cgcaagccga tgttgcttat tatcaggtac tggcacagga
    21001 gaaacgccag gaggccggac gtcgtaaccg tctcggtgtg caggcgatgt ctcgcgaaga
    21061 gatcgaccag gctaacaacg tactacaaac ggttctgcat cagttagcga aagcgcaggc
    21121 gacccgcgat ctggcaaaac tggatcttga acgcacggtg atccgcgcgc cagcagatgg
    21181 ctgggtgacc aacctcaacg tctataccgg tgagtttatt actcgaggat caacggcggt
    21241 tgcgctggtg aaacagaact ccttctatgt actggcctat atggaagaaa ctaagctgga
    21301 aggggtgcgt ccggggtatc gtgcagagat cacgccgctt ggcagtaaca aagtgctgaa
    21361 agggactgtt gatagtgttg ccgcaggggt caccaacgcc agcagcacgc gtgacgacaa
    21421 agggatggcg actatagact ctaaccttga atgggtgcgt cttgcgcaac gtgttccggt
    21481 tcgtattcgt ctcgacaacc agcaagagaa catctggcct gcgggcacca ctgctacagt
    21541 ggtggtcact ggcaaacaag atcgcgacga aagccaggat tcgttcttcc gtaaaatggc
    21601 ccatcgcctg cgtgagtttg gttaatcacg atgggtattt tctccattgc taaccaacat
    21661 attcgctttg cggtaaaact ggcgaccgcc attgtactgg cgctgtttgt tggctttcac
    21721 ttccagctgg aaacgccacg ctgggcggta ctgacagcgg cgattgttgc cgccggtccg
    21781 gcctttgctg cgggaggtga accgtattct ggcgctattc gttatcgtgg ctttttgcgc
    21841 atcatcggca catttattgg ctgtattgcc ggactggtga tcatcattgc gatgatccgc
    21901 gcaccattat tgatgattct ggtgtgctgt atctgggccg gtttttgtac ctggatatcc
    21961 tcgctggtac gaatagaaaa ctcgtacgcg tgggggctgg ccggttatac cgcgctgatc
    22021 attgtgatca ccattcagcc ggaaccattg cttacgccgc agtttgccgt cgaacgttgt
    22081 agcgagatcg ttatcggtat tgtgtgtgcg attatggcgg atttgctctt ttctccgcga
    22141 tcgatcaaac aagaagtgga tcgagagctg gaaagtttgc tggtcgcgca atatcaatta
    22201 atgcaactct gtatcaagca tggcgatggt gaagttgtcg ataaagcctg gggcgacctg
    22261 gtgcgacgca ccacggcgct acaaggcatg cgcagcaacc tgaatatgga atcttcccgc
    22321 tgggcgcggg ccaatcgacg tttaaaagcg atcaatacgc tatcgctgac gctgattacc
    22381 caatcctgcg aaacttatct tattcagaat acgcgcccgg aattgatcac tgatactttc
    22441 cgcgaatttt ttgacacgcc ggtagaaacc gcgcaggacg tccacaagca gctcaaacgc
    22501 ctgcggagag ttatcgcctg gaccggggaa cgggaaacgc ctgtcaccat ttatagctgg
    22561 gtcgcggcgg caacgcgtta tcagcttctc aagcgcggcg ttatcagtaa cacaaaaatc
    22621 aacgccaccg aagaagagat cctgcaaggc gaaccggaag tcaaagtaga gtcagccgaa
    22681 cgtcatcatg cgatggttaa cttctggcga accacacttt cctgcattct gggcacgctt
    22741 ttctggctgt ggacgggctg gacttccggc agtggtgcaa tggtgatgat tgcggtagtg
    22801 acgtcactgg caatgcgttt gccgaatcca cgcatggtgg cgatcgactt tatctacggg
    22861 acgctggccg cgctgccgtt agggctgctc tactttttgg tgattatccc taatacccaa
    22921 cagagcatgt tgctgctgtg tattagcctg gcagtgctgg gattcttcct cggaatagaa
    22981 gtacagaaac ggcgactggg ctcgatgggg gcgctggcca gcaccataaa tattatcgtg
    23041 ctggataacc cgatgacttt ccatttcagt cagtttctcg acagcgcatt agggcaaatc
    23101 gtcggctgtg tgctcgcgtt caccgttatt ttgctggtgc gggataaatc gcgcgacagg
    23161 actggacgtg tactgcttaa tcagtttgtt tctgccgctg tttccgcgat gactaccaat
    23221 gtggcacgtc gtaaagagaa ccacctcccg gcactttatc agcagctgtt tttgctgatg
    23281 aataagttcc caggggattt gccgaaattt cgcctggcgc tgacgatgat tatcgcgcac
    23341 cagcgcctgc gtgatgcgcc gatcccggtt aacgaggatt tatcggcgtt tcaccgacaa
    23401 atgcgccgca cagcagacca tgtgatatct gcccgtagcg atgataaacg tcgtcggtac
    23461 tttggtcagt tgctggaaga actggaaatc taccaggaaa agctacgcat ctggcaagcg
    23521 ccaccgcagg tgacggaacc ggttcatcgg ctggcgggga tgctccataa gtatcaacat
    23581 gcgttgaccg atagttaagt caaaaccgac gccaaaagcg tcggtttttt catggctata
    23641 cttagcgatg aacggcagaa ctcgccgcga aacgtgacgg tggcaacaga tgaatattta
    23701 tacctttgat tttgatgaga ttgagagtca ggaggatttt tatcgcgact tcagccaagc
    23761 ctttggtctg gcgaaagata aggtacgcga tctcgactca ctatgggatg tgttaatgaa
    23821 cgatgtcctg ccgctaccac ttgagattga atttgttcat ctgggagaga aaacgcgtcg
    23881 ccgttttggc gcgttaatat tgctgtttga tgaggcagag gaagagctgg aagggcattt
    23941 gcgttttaat gttcgtcatt agcgcaaaaa aaagcccccg aaccgggggc aatatcgtcg
    24001 gacaagacga tgagggttta tttgtacagt tcagccgtag cgtgccaggt gtcaccgcta
    24061 cgagcttcag taatctggta ggccgttgcg cctttctctt ccgctttttt gttcagcatt
    24121 tcacgtatat ccattggcga agacgccaca ccacttacgg atacggtccc gattgcttca
    24181 cggttttgtg cttgtgcagc atcaatggag tcggcagcga atgcaccgaa agagagaaca
    24241 gaaagtacgc ttaatgcagc aacagtggtt ttgattttca tgatttttac ctcgacataa
    24301 tctttttgct gggtctttgt ttcgtgaccc ttatcacaaa atcaagtata cactaattat
    24361 tgaactaatt aataccacgc taactgtttc tgttgatact aagtgttaaa aatgtgaatg
    24421 ttaataacaa aaaagaatta aaaatacggt tatttatcgt taattctagt gttttcaatt
    24481 agataatgaa ttttgcataa tgtgcttttt ttaccttatg tattcattgt gtgaatgaca
    24541 tgtcgcagta aaacgcacta ttcgttaaat gtatcgcagg agaaagcagg gggttgagag
    24601 ggataagcaa cattttcccc gccgccagaa gcgacggggc agagattaaa gctcctggtc
    24661 gaacagctct aaaatcgctt cgtacaggtc tttgacggtg aaaccgttag caggggtggt
    24721 aaagatggtg tcatcgccag cgatggtgcc cagaatacct tctgctttgc ccagtgagtc
    24781 cagcaggcga gcaattaact gcgccgcgcc agggctggta tgaatcacga caactgcatc
    24841 gttgtagtcg atatccagca ccagattttt caatggactg gaggtggttg gtacacccag
    24901 ttcagctggc aggcagtaaa ccatttccat tttggcattg cgtgtacgta cagcaccaaa
    24961 cttggtcaac atccgcgaga ctttagactg attaatattg tcaaagcctt gctcctgcaa
    25021 cgcggcgacg atttcgccct gggagctaaa tttctcttct ttaagtaatg ctttaaatgc
    25081 tttaactagt tcttcttgct tagccgagct tcgcataagt cacccgatat ggtggttgat
    25141 acaacattat tgtgcataca gatgaatttt tatgcaaaca gtcagccctg aagaaggctg
    25201 aaataatgtt atgaaagagc gggattttat caaatttcgt tattgagaaa catgcctgcg
    25261 tcacggcatg caaattctgc ttaaaagtaa attaattgtt atcaaattga tgttgttttg
    25321 gctgaacggt agggtatatt gtcaccacct gttggaatgt tgcgctaatg cataagcgac
    25381 tgttaattac gtaagttagg ttcctgatta cggcaattaa atgcataaac gctaaacttg
    25441 cgtgactaca cattcttgag atgtggtcat tgtaaacggc aattttgtgg attaaggtcg
    25501 cggcagcgga gcaacatatc ttagtttatc aatataataa ggagtttagg atgaaagtcg
    25561 cagtcctcgg cgctgctggc ggtattggcc aggcgcttgc actactgtta aaaacccaac
    25621 tgccttcagg ttcagaactc tctctgtatg atatcgctcc agtgactccc ggtgtggccg
    25681 tcgatctgag ccatatccct actgctgtga aaatcaaagg tttttctggt gaagatgcga
    25741 ctccggcgct ggaaggcgca gatgtcgttc ttatctctgc aggtgtagcg cgtaaaccgg
    25801 gtatggatcg ttccgacctg tttaacgtta acgccggcat cgtgaaaaac ctggtacagc
    25861 aagttgcgaa aacctgcccg aaagcgtgca ttggtattat cactaacccg gttaacacca
    25921 cagttgcgat tgctgctgaa gtgctgaaaa aagccggtgt ttatgacaaa aacaaactgt
    25981 tcggcgttac cacgctggat atcattcgtt ccaacacttt tgttgcggaa ctgaaaggca
    26041 aacagccagg cgaagttgaa gtgccggtta ttggcggtca ctctggtgtt accattctgc
    26101 cgctgctgtc acaggttcct ggcgttagtt ttaccgagca ggaagtggct gatctgacca
    26161 aacgtatcca gaacgcgggt actgaggtgg ttgaagcgaa agccggtggc gggtctgcaa
    26221 ccctgtctat gggccaggca gctgcacgtt ttggtctgtc tctggttcgt gcactgcagg
    26281 gcgaacaagg cgttgtcgaa tgtgcctacg ttgaaggcga cggtcagtac gcccgtttct
    26341 tctctcaacc gctgctgctg ggtaaaaacg gcgtggaaga gcgtaaatct atcggtaccc
    26401 tgagcgcatt tgaacagaac gcgctggaag gtatgctgga tacgctcaag aaagatatcg
    26461 ccctgggcga agagttcgtt aataagtaat tgattagcga ataataaaaa accggagcac
    26521 agactccggt ttttgttttg agcactcgac ttaattggtt gccggatatt cctgaatggt
    26581 gacctgcagc gttaactgct tatcatcacg catcactaca accgggatca ccgaaccagg
    26641 gcgaatttcc gccacctgat ccatcgtctc cagagcagag atggccggtt tgttatccac
    26701 cgaaataatc agatcgttga cctgaatacc cgcattcgcc gccgggccgt caggtgacac
    26761 ttcattaacc acgatccctt gcagttgatc tataccaccg ccctgcgcgt gcagtggtgc
    26821 gatctcgcgt ccgccgatac caatgtagcc gcggatcacg cgaccatcgc ggatcagctt
    26881 atccataatt ttggttgcta actggaaagg aatcgcaaag ccgatacctt ccggcgtttc
    26941 gccatcgtta ctcttatcaa acgacagcgt gttaatgccc atcagttcgc ccagcgagtt
    27001 caccagcgcg ccgccagagt taccgtggtt aatggaagca tcggtttgta ggaagttttg
    27061 ccgcccggtc gggttcagac cgattcgacc cgtggcacta ataatcccct gggtaatggt
    27121 ctgcccgagg ttgtacgggt taccgatcgc cagtactacg tcgccaatgt gcggtacgcg
    27181 acgtgcattg atcggaatgg taggtaaacc gccagtggca ttaattttaa gtaccgccag
    27241 atcggttaga gagtcagatc ccaccagcaa tgcttcaaat acacgaccat cctgtaaggc
    27301 gacgatgatc tgatcggcgt cgttgatgac gtgtttattg gtgatgatat aaccgcgttg
    27361 atccatgatt acaccggatc ccagggtgcg gatctcaagc tggttgtgag agttggtgtt
    27421 caaaccacgg ttgtaaacgt taaccaccgc tggcgcggcg cggcgaaccg ccagattata
    27481 gctggcaggc gtctcatcgg tactgtcaaa ttgcggagtg gaaagcgggt taaggctgcg
    27541 cagcgaaggc atggcaacca gcagaatagc gccgacaatt aatccaatcg caacggaacg
    27601 taagagcttc acaaacatga tggaggcgtc attaaaaaag ggaacggcag cagcatacca
    27661 cgagttaacc ggacatcaca cgtaagcctg atgcccggtt tacgacatta acgcatcagc
    27721 agatagatgc tttcattgcc gcgtacaatt tgcagggcga tgatggccgg ttttgccgcc
    27781 agcactttac gcatttcagc aatcgagttc acccgatcgc ggttgacgcc aatgatcaca
    27841 tcgtcttttt gcaagccagc ctgagcagct gggcttcctt tgacaacttc atcgatctta
    27901 atacctttgc cgccatcttt tagctgacca tcgctcaacg ttgcaccttc cagcgctggc
    27961 gtgatcattt cagcgctggc cgacgaagag gtgctggtat cgagcgtcac ttctacttcc
    28021 agtggcttgc cgttacgcag caggccaagc ttcactttcg tgcccggctc ggtggtcgcg
    28081 atacgagagc gcaactcagc aaagctattc agcggtttgc cgttgaggct ggtaataata
    28141 tcgcccgctt tgacgcccgc tttcgctgag ccagaacctg gcaacacttc gctgacaaac
    28201 gcgccacgct gcacgtcaag gttgaaggct ttggcgatat cggcactcat ctcggtgcct
    28261 ttgatgccta acaaaccgcg tttgatttca ccaaagtcga taagctgctg cgccagtgtt
    28321 cgcgccatat tactggggat ggcaaatcca atcccgacgc tcccgccgcc aggcgcaagg
    28381 attgcagtgt tgatgccaat taactcaccg ttaaggttta acagtgcacc gccggagtta
    28441 ccgcggttaa tggaagcatc tgtctggata aagttttcca gaccttcaag attcaacccg
    28501 ctgcgtccta atgcggaaac aatgccagag gtggcggttt gcccaaggcc aaatgggtta
    28561 ccgaccgcta cggcaaaatc accgacgcgc aatttatcgg agtcggcaat agcgatttgc
    28621 gttaatttgc tcgggttttg aatttgtaac agggcgatat cgctctggtc atcgctacca
    28681 atcagttttg catcaaactc gcgcccatca ttgagctgaa tactgatttt ctgtgcctga
    28741 ttaatcacat ggttgttggt cagcacatag cctttactgg cgttgatgat gacaccggaa
    28801 cctaaacctt cgaagggttg tgcaggttga tccggtaaat catcaccaaa aaactttttg
    28861 aattcttccg ggattttctg tccctgactg gccgttcctt ccacccgtac gctcaccact
    28921 gccggaagca ctttttccag cattggagcc agactgggga gaggggcctg atcggcaacc
    28981 tggcctggaa tcgacgcgac ggcctgaaat gacgccgaga gagttaaccc gacacttaac
    29041 gctaatgcac tcaacagctg ggtttgtttt ttcattattc ctgctctcgt acctgaatga
    29101 taagaaaaga gattcaaaac agtttgatgt tattgaattt tcagacggct aacaatgaga
    29161 caccagatta aatgatagtc ggggagggag agaagaggag ggcgcaatgg ctgcgcccga
    29221 aaaataaatt agtcgcgctt cgcgccagta cgcagcaggc cggatgcgcc ttcagaatag
    29281 tcgcgaggca tctgcaccgg tgcctgatcg ttgctggctt cagactctgc cagacgatta
    29341 cggaacgggt ttgcttcagc agacagttcc ggcagcaggc tgctggagct tttcgccatg
    29401 tgctgataca gctggcgata gtcgtgcgcc atggtatcca gtaattccgc gctgcgggca
    29461 aagtggctaa ccagctcttc gcgatactcg tccagttcag ctttattctt ttccagttcg
    29521 tactgcaacg cctgttgctg gcgtagttta cgattaccaa aacgcatggc cacagcacca
    29581 ataatgatgc cgacgactaa cccaattagc gcatattccc aggtcatgaa catctcccgt
    29641 tgtcttttgt ttccgtaggg tgttggcttc aggctccatg cctgcggctg attatgccac
    29701 tataaccgtt aattccacag aagtggaatc ccgactgcat atcgcgtagt gtagaacggc
    29761 ctttttttcg tcaaccgtga acaacggcgc accgattatt caaggaataa caataagatc
    29821 atgcaaagcg ttaccccaac atcgcaatac ctgaaggcgc tcaatgaagg cagccatcaa
    29881 cccgacgacg ttcaaaaaga ggccgtcagc cgcctggaaa ttatttatca ggaactcatc
    29941 aatagcacgc caccagcccc caggacgagt gggctaatgg cgcgggtcgg taagctgtgg
    30001 ggtaaacgcg aagacacaaa gcatacgcca gtgcgtggct tatatatgtg gggcggtgta
    30061 ggacgcggga aaacctggct gatggacctt ttctatcaaa gcctgccggg agagcggaaa
    30121 cagcgcctgc actttcaccg ttttatgatg cgggtgcatg aagagctaac tgccttacag
    30181 gggcagaccg atccgctgga aattattgcc gatcgcttta aagccgaaac tgacgtgctc
    30241 tgttttgacg aattttttgt ttctgatatt accgacgcca tgctacttgg cggtctgatg
    30301 aaagccctgt tcgcccgcgg cattaccctg gtagcgacgt caaatattcc gccggatgaa
    30361 ctttatcgaa atggcctgca acgtgcgcgt tttctgcctg caatcgatgc cattaaacag
    30421 cattgtgatg taatgaacgt ggacgctggt gttgattatc gactgcgtac actcactcag
    30481 gcgcatctgt ggctttcgcc actcaacgat gaaacccggg cgcagatgga taaactatgg
    30541 ttggcgctgg cgggggcgaa acgagaaaat tcaccgacgt tagaaatcaa ccatcggcca
    30601 ttggcgacaa tgggcgtcga gaaccagacg ctggcggtct cttttactac gctgtgcgtc
    30661 gacgcccgca gtcagcatga ctatattgcg ctctcacgtc tctttcatac ggtcatgttg
    30721 tttgatgtac cagttatgac gcggttgatg gagagcgaag cgcggcgctt tattgcgctg
    30781 gtggatgagt tttacgagcg ccatgtcaaa ttagtggtga gtgcagaagt gccgctgtat
    30841 gaaatttatc agggcgatcg gctgaagttt gagttccagc gttgcctgtc acgtctgcaa
    30901 gagatgcaaa gcgaagagta tctgaagcgc gagcatttgg cgggttaaaa cctgtcacaa
    30961 atcacaaaaa ggggtcgatc tttgaccccg acttctctat aatcctgcga ccccacgtta
    31021 caagaaagtt tttttcccaa aactttttgt gtgctggcat aggctattcg aaggggtagg
    31081 tttgccggac tttgtcgtgt gaacctcaac aattgaagac gtttgggtgt tcaccaacgt
    31141 gtaactattt attgggtaag cttttaatga aaacttttac agctaaacca gaaaccgtaa
    31201 aacgcgactg gtatgttgtt gacgcgaccg gtaaaactct gggccgtctg gctactgaac
    31261 tggctcgtcg cctgcgcggt aagcacaaag cggaatacac tccgcacgta gataccggtg
    31321 attacatcat cgttctgaac gctgacaaag ttgctgtaac cggcaacaag cgtactgaca
    31381 aagtgtacta tcaccacacc ggccacatcg gtggtatcaa acaagcgacc tttgaagaga
    31441 tgattgctcg ccgtcctgag cgtgtgattg aaatcgcggt taaaggcatg ttgccaaaag
    31501 gcccgctggg tcgtgctatg ttccgtaaac tgaaagttta cgcgggtaac gagcacaacc
    31561 acgcggcaca gcaaccgcaa gttcttgaca tctaatcggg attataggca atggctgaaa
    31621 atcaatacta cggcactggt cgccgcaaaa gttccgcagc tcgcgttttc atcaaaccgg
    31681 gcaacggtaa aatcgtaatc aaccaacgtt ctctggaaca gtacttcggt cgtgaaactg
    31741 ctcgcatggt agttcgtcag ccgctggaac tggtcgacat ggttgagaaa ctggacctgt
    31801 acatcaccgt taaaggtggt ggtatctctg gtcaggctgg tgcgatccgt cacggtatca
    31861 cccgcgctct gatggaatac gacgagtccc tgcgttctga actgcgtaaa gctggcttcg
    31921 ttactcgtga cgctcgtcag gttgaacgta agaaagtcgg tctgcgtaaa gcacgtcgtc
    31981 gtccgcagtt ctccaaacgt taattggctt ctgctccggc agaaaacaat ttgcgaaaaa
    32041 acccgcttcg gcgggttttt ttatggttaa attctgaatc agcgtaaaaa ttggaaagtt
    32101 gctttttgct accgtctgac agacaggcaa aacaaaaacc acatcgccaa taagggacta
    32161 agtcaactat ttcagactaa agcgcatctc tttttcccca tttccggcat cgactcacca
    32221 caaaggtcgc aaaatctggt aaactatcat ccaattttct gcccaaatgt cgggtattgc
    32281 tcattttttg tttgattttc gaacaaagag agtcgttcct tactgggtaa cacaacttct
    32341 gactggccac ctggtggctg gtagcagtaa aaattctgac tatacctgga ggttttcatg
    32401 gctgtcgctg ccaacaaacg ttcggtaatg acgctgtttt ccggtcctac tgacatctat
    32461 agccatcagg tccgcattgt gctggctgag aaaggtgtaa gtttcgagat cgaacacgtg
    32521 gaaaaggaca atccgcctca ggatctgatt gacctcaacc cgaatcagag cgttccgacc
    32581 ctggtggatc gtgagctgac cctgtgggaa tctcgcatca ttatggaata tctggatgag
    32641 cgtttcccgc atccgccact gatgcctgtt tacccggtag ctcgcggtga aagccgtctg
    32701 tacatgcatc gtatcgaaaa agactggtac acgctgatga acaccatcat caacggttca
    32761 gcttctgaag cagatgccgc acgtaagcaa ctgcgcgaag aactgctggc gattgcgccg
    32821 gtcttcggtc agaagccgta cttcctgagc gatgagttca gcctggtcga ttgctatctt
    32881 gctccgctgc tgtggcgtct gccgcaactg ggcatcgagt tcagcggccc gggtgcgaaa
    32941 gagctgaaag gctatatgac ccgcgtcttt gagcgtgact ctttccttgc ttctttaact
    33001 gaagcagaac gtgaaatgcg tctgggccgg agttaatctg tatggatttg tcacagctaa
    33061 caccacgtcg tccctatctg ctgcgtgcat tctatgagtg gttgctggat aaccagctca
    33121 cgccgcacct ggtggtggat gtgacgctcc ctggcgtgca ggttcctatg gaatatgcgc
    33181 gtgacgggca aatcgtactc aacattgcgc cgcgtgctgt cggcaatctg gaactggcga
    33241 atgatgaggt gcgctttaac gcgcgctttg gcggcattcc gcgtcaggtt tctgtgccgc
    33301 tggctgccgt gctggctatc tacgcccgtg aaaatggcgc aggcacaatg tttgagcctg
    33361 aagctgccta cgatgaagat accagcatca tgaatgatga agaggcatcg gcagacaacg
    33421 aaaccgttat gtcggttatt gatggcgaca agccagatca cgatgatgac actcatcctg
    33481 acgatgaacc tccgcagcca ccacgcggtg gtcgaccggc attacgcgtt gtgaagtaat
    33541 acaaaaacag gccctggtgg cctgttttgt cttttaatgg cttaataaaa tagcgtgatt
    33601 gtggcaatca tattcaccac gaaaccgact gccatgggga tcgctgttcg cttcaccacc
    33661 gcaaaaggcg aaacgcctgc aattcctgac gtaacgacca ccaccgcagt aatcggcgaa
    33721 accgcgcgcg ctagcgtcgt ggcaaaatgc atcggcataa tcattacaac cgctggtaca
    33781 tgtagtccgg ctgcgatatt cggaataaga ctggcaaatg acataaacgg cgcattgcca
    33841 gagcccatca caatggcaca aatggcaatg actagcgcca taataatcat cacgccaata
    33901 ccgcccagac cagaatgctc cgcacccctg ataaccgcat caacagtgcc aatcgtcgtt
    33961 aagcctttcg caaatatttc acccgcgacc accagcgtta ccacgttggc aaactgcgta
    34021 cccatgccgt caaaaaacgc ctgcacatcg tccattgttt cgcgcaagtt atgcttgcgg
    34081 aagaactcaa caaacatcgt caccgtcaaa ctcagtaaca tcaccaccac cagatgcagt
    34141 tctgactgca tcagcccgac gtgggcgagg aacagcgagc cgagcatcag gattaacggc
    34201 atcacaggta aaatggcgta atagagcggc gggacattat cgagagcttt ttgctctgct
    34261 tgttcgtgat tgatattttt atcttttttg tcaaaagcgc gttgcacgaa aaagtgggag
    34321 atcgccaccg agataatgac gcaagaggcg accggaagct ggtagtggaa gaagtaagtg
    34381 gcaattttca ttcccgcgac ctgggctgca aaaatggagt tcgtttccag aattccccac
    34441 tcaatggaca ttgtggttgc gataaccgcc accgcagaga gacgacttac tcccagactc
    34501 accagtgtcg gaaataaggt gaccatcagc aacataccca gaccggaggc gctggtaata
    34561 aactgcgcca tgatttgacc gatgacgtaa gttgccgaca gaataatata cggcgagcga
    34621 atgagtttta acgggcggct taacaagctc accatcgcgc gactggcccc tatgcgctcc
    34681 atgtagcggg cataaccgcc caccgccata atcgatagcc ccaatccggc aatgcggttg
    34741 gacagcatgc gcaggatttc attataaata tcgacgatca agtagccgct gcttttggcg
    34801 ggatcgagta caccaccgaa ccctaaccag acaccgcaca tcatcaggaa gatacctgcg
    34861 gcagccagca ccacctgagg tttgtagttt ttcaggatca aatagcccat cgtaattaat
    34921 acgatgacag atataattat gccgaacatt gttgaactcc gtgtcaaaag aaaacggtca
    34981 atcccataaa cggcagattg aaaacaatga tgttatattt tttgcaagtc tatttatggt
    35041 acggatgtcg tgtttttaat tgtaggtgag gtgatttttc attaaaaaat atgcgcttat
    35101 gattat
//
LOCUS       DBANUU010000038        34890 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000038 DBANUU010000000
VERSION     DBANUU010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.038"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(75..806)
                     /gene="rlmB"
                     /locus_tag="ACIE68_001780"
     CDS             complement(75..806)
                     /gene="rlmB"
                     /locus_tag="ACIE68_001780"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFX5908108.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(986..3427)
                     /gene="rnr"
                     /locus_tag="ACIE68_001781"
     CDS             complement(986..3427)
                     /gene="rnr"
                     /locus_tag="ACIE68_001781"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFX5908109.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3466..3891)
                     /gene="nsrR"
                     /locus_tag="ACIE68_001782"
     CDS             complement(3466..3891)
                     /gene="nsrR"
                     /locus_tag="ACIE68_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFX5908110.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKSASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4096..5394)
                     /gene="purA"
                     /locus_tag="ACIE68_001783"
     CDS             complement(4096..5394)
                     /gene="purA"
                     /locus_tag="ACIE68_001783"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFX5908111.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5498..5695)
                     /gene="yjeT"
                     /locus_tag="ACIE68_001784"
     CDS             complement(5498..5695)
                     /gene="yjeT"
                     /locus_tag="ACIE68_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFX5908112.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5777..6781)
                     /gene="hflC"
                     /locus_tag="ACIE68_001785"
     CDS             complement(5777..6781)
                     /gene="hflC"
                     /locus_tag="ACIE68_001785"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFX5908113.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6784..8043)
                     /gene="hflK"
                     /locus_tag="ACIE68_001786"
     CDS             complement(6784..8043)
                     /gene="hflK"
                     /locus_tag="ACIE68_001786"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFX5908114.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8129..9409)
                     /gene="hflX"
                     /locus_tag="ACIE68_001787"
     CDS             complement(8129..9409)
                     /gene="hflX"
                     /locus_tag="ACIE68_001787"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFX5908115.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9486..9794)
                     /gene="hfq"
                     /locus_tag="ACIE68_001788"
     CDS             complement(9486..9794)
                     /gene="hfq"
                     /locus_tag="ACIE68_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFX5908116.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9880..10830)
                     /gene="miaA"
                     /locus_tag="ACIE68_001789"
     CDS             complement(9880..10830)
                     /gene="miaA"
                     /locus_tag="ACIE68_001789"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFX5908117.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10823..12670)
                     /gene="mutL"
                     /locus_tag="ACIE68_001790"
     CDS             complement(10823..12670)
                     /gene="mutL"
                     /locus_tag="ACIE68_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFX5908118.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12680..14017)
                     /gene="amiB"
                     /locus_tag="ACIE68_001791"
     CDS             complement(12680..14017)
                     /gene="amiB"
                     /locus_tag="ACIE68_001791"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFX5908119.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14036..14497)
                     /gene="tsaE"
                     /locus_tag="ACIE68_001792"
     CDS             complement(14036..14497)
                     /gene="tsaE"
                     /locus_tag="ACIE68_001792"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFX5908120.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14469..16016)
                     /gene="nnr"
                     /locus_tag="ACIE68_001793"
     CDS             complement(14469..16016)
                     /gene="nnr"
                     /locus_tag="ACIE68_001793"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFX5908121.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16015..17154
                     /gene="queG"
                     /locus_tag="ACIE68_001794"
     CDS             16015..17154
                     /gene="queG"
                     /locus_tag="ACIE68_001794"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFX5908122.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17137..17190)
                     /locus_tag="ACIE68_001795"
     CDS             complement(17137..17190)
                     /locus_tag="ACIE68_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908123.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17424..17499)
                     /locus_tag="ACIE68_001796"
     tRNA            complement(17424..17499)
                     /locus_tag="ACIE68_001796"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17464..17466),aa:Gly,seq:gcc)
     gene            complement(17710..18255)
                     /gene="orn"
                     /locus_tag="ACIE68_001797"
     CDS             complement(17710..18255)
                     /gene="orn"
                     /locus_tag="ACIE68_001797"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFX5908124.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18350..19402
                     /gene="rsgA"
                     /locus_tag="ACIE68_001798"
     CDS             18350..19402
                     /gene="rsgA"
                     /locus_tag="ACIE68_001798"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFX5908125.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19499..20467
                     /gene="asd"
                     /locus_tag="ACIE68_001799"
     CDS             19499..20467
                     /gene="asd"
                     /locus_tag="ACIE68_001799"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFX5908126.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20489..23812
                     /gene="mscM"
                     /locus_tag="ACIE68_001800"
     CDS             20489..23812
                     /gene="mscM"
                     /locus_tag="ACIE68_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFX5908127.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(23963..25465)
                     /gene="yjeM"
                     /locus_tag="ACIE68_001801"
     CDS             complement(23963..25465)
                     /gene="yjeM"
                     /locus_tag="ACIE68_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFX5908128.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGMTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSISMWMQCGLVTIFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(25684..26661)
                     /gene="epmA"
                     /locus_tag="ACIE68_001802"
     CDS             complement(25684..26661)
                     /gene="epmA"
                     /locus_tag="ACIE68_001802"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFX5908129.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIDALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            26986..28794
                     /gene="frdA"
                     /locus_tag="ACIE68_001803"
     CDS             26986..28794
                     /gene="frdA"
                     /locus_tag="ACIE68_001803"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFX5908130.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            28787..29521
                     /gene="frdB"
                     /locus_tag="ACIE68_001804"
     CDS             28787..29521
                     /gene="frdB"
                     /locus_tag="ACIE68_001804"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFX5908131.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            29532..29927
                     /gene="frdC"
                     /locus_tag="ACIE68_001805"
     CDS             29532..29927
                     /gene="frdC"
                     /locus_tag="ACIE68_001805"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFX5908132.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            29938..30297
                     /gene="frdD"
                     /locus_tag="ACIE68_001806"
     CDS             29938..30297
                     /gene="frdD"
                     /locus_tag="ACIE68_001806"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFX5908133.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            30360..31493
                     /gene="ampC"
                     /locus_tag="ACIE68_001807"
     CDS             30360..31493
                     /gene="ampC"
                     /locus_tag="ACIE68_001807"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFX5908134.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            31582..32115
                     /gene="blc"
                     /locus_tag="ACIE68_001808"
     CDS             31582..32115
                     /gene="blc"
                     /locus_tag="ACIE68_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFX5908135.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(32112..32429)
                     /gene="sugE"
                     /locus_tag="ACIE68_001809"
     CDS             complement(32112..32429)
                     /gene="sugE"
                     /locus_tag="ACIE68_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFX5908136.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(32604..32750)
                     /gene="ecnB"
                     /locus_tag="ACIE68_001810"
     CDS             complement(32604..32750)
                     /gene="ecnB"
                     /locus_tag="ACIE68_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFX5908137.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(32861..32986)
                     /gene="ecnA"
                     /locus_tag="ACIE68_001811"
     CDS             complement(32861..32986)
                     /gene="ecnA"
                     /locus_tag="ACIE68_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFX5908138.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(33038..33604)
                     /gene="efp"
                     /locus_tag="ACIE68_001812"
     CDS             complement(33038..33604)
                     /gene="efp"
                     /locus_tag="ACIE68_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFX5908139.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            33646..34674
                     /gene="epmB"
                     /locus_tag="ACIE68_001813"
     CDS             33646..34674
                     /gene="epmB"
                     /locus_tag="ACIE68_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFX5908140.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         8288 a         9556 c         8892 g         8154 t
ORIGIN      
        1 tacgtaatta tcttaccagc tatagacaaa aaaaaaccat ccaaatctgg atggcttttc
       61 ataattcaga cgaactagct gcgctgacga accgcttcaa ataagcaaat tccggttgca
      121 accgaaacgt tcagggaaga aacacttcct gccattggga tgctgatcaa ctcatcgcaa
      181 tgttcacggg tcaggcgacg cataccttca ccttccgcgc ccatcaccag cgccaggcgt
      241 ccggtcattt tgctctgata gagtgtatga tccgcctcgc ctgccgtacc gacgatccag
      301 atattctctt cctgcaacat acgcatggtg cgcgcaaggt tagtcacccg aatcagtgga
      361 acgctttctg ccgcgccgca ggctactttt ttcgccgtag cgttgagctg tgcggagcga
      421 tctttcggca caatcaccgc gtgaacgcca gcagcgtccg cgctacgcag gcacgcgccg
      481 aggttgtgcg gatcggttac accgtcgagg atcagcagga acggttgatc gagcgaagcg
      541 atcagatccg gcagatcgtt ttcctgatac tgacgtcctg gcttcacgcg ggcgataatg
      601 ccctgatgca cggcaccgtc gcttttctcg tcgagatatt ggcggtttgc caactggata
      661 accacgccct gagactcaag ggcgtgaatc agcggtaaca gacgtttatc ttcacggcct
      721 tttaaaataa agacttcctg aaaacgttca ggggcgcgct ccagcagggc ctgcactgcg
      781 tggatgccgt aaatcatttc gctcattaat gtactcgttg tttacgtttt gcctgatgca
      841 ctacgtttat ccggcctaca tgatctctgc aatatattga atttgcgtga ttatgtaggc
      901 cggataaggc gttcacgccg catccggcat gaacaaagcg cattttgtca gcaatctaac
      961 cctcttcttt taaagagggt attgatcact ctgccacttt tttcttcgcc gcacgcttcg
     1021 ctttggtcgc tgcggctatt ttctgcgttt tcgccgatgg ctttttcgct tttctcgcgt
     1081 ctttcttcgc cgctttcggc ttcgtttttt tctcaccgcg gaaggcgctg tctggctcaa
     1141 agtttacctt tttaccgacc tgacgacgct tgccgccttt tttgcctgca tcgccttttt
     1201 tcgctttctc gcgcgccgtt ttaccgacgt tgcgcggtgc gcgttcactg gagatcagac
     1261 taaagtcgat tttgcgctcg tccatattaa ccgcttcgac tcgaacttcc acgcgatcgc
     1321 ccaggcgata agtctggccg ctggattccc ccatcaggcg ttgccctacc tggtcaaagc
     1381 gatagtagtc attgtccagc gaagagacat ggaccagacc atcaatgaac aagtcgtcca
     1441 gacggacgaa gaagccaaag ccagtgacgc tggaaattac gcctttaaag acgttaccta
     1501 cctggtcgag catgaagtca cacttcagcc agtcagcgac atcgcgcgtt gcttcgtcgg
     1561 cacgacgttc cgccatcgaa cagtgctgac ccagttgcaa catctcttcc atcgaataat
     1621 ggtagccgcc ggtttcagtg gtgttgccct gatgcccctg ctctttcgcc agcagatatt
     1681 taatggcgcg gtgcagcgtc aggtcaggat aacgacgaat cggcgaagta aagtgcgcat
     1741 aggactgcaa tgccagaccg aagtgaccac ggttttctgg atcgtaaatc gcctgtttca
     1801 tcgagcgtag cagcatggtt tgcagcattt ctgcatcagg acggtcggca accgactcca
     1861 gcagttccgc gtagtcacgc ggttccggct tgttaccacc cggcagctcc agccccagct
     1921 ccgccagcac tgaacggaaa gaggtaatcg cttcggtgct cggcttgtcg tgaatacgga
     1981 acagtgccgg ttctttcgct ttctcaacga aacgcgccgc cgagatattc gccagaatca
     2041 tgcactcttc aattaatttg tgcgcgtcgt tacgctgggt ctgttcgata cgttcaatac
     2101 ggcgttcagc gttgaaaatg aacttcgctt cttcgctctc aaatgagatc ccaccgcgtt
     2161 cttcacgggc tttatccagc actttataga ggttatgcaa ctcttcgaga tgcttaacca
     2221 gcggggcgta ctgctcgcgc agatcctgat cgccctgcag aatatgccag actttggtgt
     2281 aggtcagacg cgcgtgagag ctcatcaccg cttcgtagaa tttgtagccc gtcaggcggc
     2341 ctttcgacga aaccgtcatc tcgcacacca tacacaggcg gtctacctgc gggttgagcg
     2401 aacacaggcc gttagagagc acttccggca gcatcgggat aacctgcgaa gggaagtaca
     2461 ccgacgtgcc acggttacgc gcttctctgt ccagcggcgt tggcggacgc acatagtagc
     2521 tgacgtcggc aatcgcgacc cataaacgcc agccgccgcc gcgttttttc tcgcagtaaa
     2581 ctgcatcgtc aaagtcacgg gcgtcttcgc catcaatggt gaccagcggt aaatcgcgca
     2641 gatcaacacg gcccgctttt gcttcttccg gcacttcttc tttcagcccg gcaacctgtt
     2701 gctcaacagc ctgcggccag atgtacggaa tttcatgggt acgcagagcg atatcaaccg
     2761 ccatgccggt gcccatattg tcgcccagca cttcgacgat tttacccacc gctttggtgc
     2821 ggcgagtcgg acgctgagtc agttcgacta cgaccacaaa gcccatccgc gcgcccatga
     2881 tctgatcggg cgggattaag atatcgaagc tcagacggct atcgtcagga accacaaagc
     2941 cgacgcccgc ttcagtaaag tagcgaccaa caatctggct ggtttttggc accagtacgc
     3001 ggacaatacg cgcttcacga cgacctttac ggtcagcgcc cagcggctgc gccagcacct
     3061 gatcgccatg aatgcaggtt ttcatctgct cgctggagag atacaaatca tctttacgcc
     3121 cttcaacccg cagaaagccg tagccatcac ggtggccaat aacggtacct ttcaccaggt
     3181 cgaggcgttc cggcagcgca tagcactgac gacgagtgaa gaccagttga ccatcgcgct
     3241 ccatcgcgcg caggcggcga cgcaggcctt caagctgctc ttcgccttca atgtgcagtt
     3301 ctaccgccag ctcatcacgg ctggccggtt tttcacgttt ggttaaatgt tcgaggataa
     3361 attcccgact agggatggga ttcgcgtatt tttcagcttc gcgttcctgg aaaggatctt
     3421 gtgacatctc ggttcctccg ttgtcatctc tgatgaagat tttcgtcact ccaccagcaa
     3481 taatttataa agcggttgat tctcttcaac caaatcggca agcgtgtagt tatccagttc
     3541 cgtaagaaaa ctttgcacgg ccttagaaag cgcctgtttc aaccgacagg caggtgtaat
     3601 gtggcaaaac tcactgctgc aattcaccag cgataagggc tccagctcgc gcaccacatc
     3661 accaatacgt atcgcactcg ccgatttacc caggcgaatg ccgccatttt ttccacgaac
     3721 agcagtcacg tagccggcac gactaagttg attgattatt ttgaccatat gattacggga
     3781 gacgccgtag acgtcagtca cttcagaaat actggtcatc cgcccttctg gcaatgacgc
     3841 catgtagatc agcgcacgta atccgtaatc agtgaaactc gttaactgca catcaacctc
     3901 aaaagggaaa tcgggaaaaa atacatttat attgatgata aaccagccac aagctgtgtc
     3961 gctaatttat ttcagttcgg ggaaggaaaa aagcgaggat ttaacactgt gtcggatagc
     4021 gtaatggctt aaccgacaca gcaatgtatc acactgttag cccggcaggc aaaatatctg
     4081 ccaggcgtac cagaattacg cgtcgaacgg atcgcgcaga atcatggttt cagtacgatc
     4141 cggaccggta gagatgatat cgatcggcac accagtcagc tcttcaatac gcttgatgta
     4201 gttcagcgcc gcctgcggca gaccgctacg atctttcacg ccgaaggtgg attcagacca
     4261 gcccggcatg gtttcgtaaa tcggctctac acctttccag tcgtcagctg ccagcggagt
     4321 ggtagtcact tcgcgaccat ccggcatacg gtaagccacg cagagtttaa cctctttcag
     4381 gccatccaga acgtccagtt tagtcaggca gaagccagac agggagttca gctgtaccgc
     4441 acgacgaacg gcaacggtgt ccagccagcc ggtacgacga cgacgccccg tagttgcgcc
     4501 gaattcgtta ccctgcttgc agaggaactc gccagtttca tcaaacagtt cggtcgggaa
     4561 cggacctgca cctacacgag ttgagtaagc tttgaggata cccagaacat aatcaacata
     4621 acgcgggccc aggccggaac cggtcgccac gccaccagca gtggtgttgg aagaagttac
     4681 gtacggataa gtaccgtggt cgatatccag cagcgtaccc tgcgcacctt cgaacatgac
     4741 gaaatcgcca cgctgacgcg cctggtcgag caggtcagaa acgtcaacca ccatagaagt
     4801 caggatgtcg gcaacagcca tcgtatcatc cagaactttc tggtaatcaa ccgcttcagc
     4861 tttgtagtag ttaaccaact ggaagttgtg atattccatc acttctttca gtttttcagc
     4921 gaaggtttct ttgtcgaaaa ggtcgccaac acgcagaccg cgacgtgcta ctttatcttc
     4981 ataagcaggc ccgataccac gaccggtggt gccgatcgct ttcgcgccac gcgctttctc
     5041 acgcgcgtta tccagcgcaa cgtgataatc aaggatcagc ggacatgctt cagacagcag
     5101 cagacgctca cgaacgggga tgccacggtc ttccagttct ttcatctctt tcatcagcgc
     5161 ggccggagac agcacaacac cgttaccgat gatgctggtt acattctcgc ggagaatacc
     5221 tgatggaata agatggagaa cggttttttc accgttgatt acgagagtat ggcctgcgtt
     5281 gtgaccgccc tggtagcgta caacatattt agcccgttca gtcagaagat cgacgatctt
     5341 acctttacct tcgtcacccc attgggtgcc cagtacgacg acgttgttac ccatttttca
     5401 aaatcaccgt ttgcttaaaa atggattcta ccatcgcttt ttcagagtta cagcactttt
     5461 tgcactcaaa aatgacctcg atcagtctgt tttttgttca gccaatcgtt ttcctcaaca
     5521 tgtagtagac cacaacgccc gcaaccacaa gtccaccgcc aaaacgacgt aaaatattat
     5581 cgggcaaatt ggtcatcgca gagatcatct tcttccatgc cttcgggtaa agcatcggcc
     5641 ctaaaccttc cagtaccaaa accagggcaa gcgccagcca gattgtcgaa ttcattcatt
     5701 atccttatag aaaaagaaaa ccaccgacat ccctgaggat gcggtggctt tattgacctg
     5761 taccgcagtc gttatattaa cgcgttgcgg aagtcggcgt cttcatgtag cggaagaaat
     5821 cgctatccgg gctcatgacc atcacgtcct gattgccaga gaagctgttc tcataagcac
     5881 gcaggctacg gatgaatgcg tagaagtccg gatctttact gaatgcatca gcaaacagtt
     5941 tggctgcttc ggcatcgcct tcaccacgca tgatgcggcc ctgacgctca gcttctgcca
     6001 gcgttctggt cacttcatag tcggcagtcg cgcgcagttt ttccgcttct tcctgacctt
     6061 gtgaacggtg acgacgcgct accgcttcac gctcggcgcg catacggttg tagatcgctt
     6121 cagacacttc ggtcggcagg ttgatctgct tgatacgcac atcgacaact tcaataccca
     6181 gcgccgccat actgttcggg ttgatgaccg gaactttgcc cttcgtctca gccgttacgc
     6241 gctctgccgc ttcggcaatg gcgttatctg ctgccggggt agtaacttca tcttctgtac
     6301 ccgcagaacc ggagttcagc gcgtcacgta cttcgagggt cagacgacca cgggaatcgg
     6361 tgacgatgtc tttcacgtcc aggcgaccaa tttcagaacg cagacggtca gagaacttac
     6421 gtttcaacag cacttccgct tgcgaaatgt cgccaccacc cgttgccagg tagtaacggc
     6481 tgaaatcgct gatgcgccat ttgatgtaag agtcgacgat caggtctttc ttctctttgg
     6541 tcacaaagcg gtcggcctgg ttgtccatgg tctgaatacg tgcgtcgagc attttcaccg
     6601 tttcaatgaa cggtatcttg aaatgcagac ccggctcata aaccagaggt ttgttgtcat
     6661 cgtcacgcag taccttacca aaacgcagcg taataccgcg ctcaccttct ttgacgacaa
     6721 agacagacat gtaaagcact accagcacga tgatgataat cgcgataact gacttacgca
     6781 tcgttattcc ccctgacgct ggtagtcgtt acgctgcgcg ttggcgcggc gttggtccat
     6841 aatatcgccc tgactggtgg acgacgtgtt gcttgctcca ctggttgtgg aggaagaggc
     6901 tggcggcaaa cgcagcagat tgctggcacc gttatcgctc ttcgccgcag gggcgttacc
     6961 acctttcagc atctggtcta acggcagaac catcaggttg ccacctttat cgttaaccag
     7021 cactttgcgg gtgttaccca acactttttc catcgtctcg atatacagac gctcgcgagt
     7081 aatttccggc gcggctttat attccggcag aagtttagca aagcgcgcca cttcaccctg
     7141 agcttccagg atggtctggg ccttgtacgc acgcgcctct tcgaggatac gttgcgcctg
     7201 accgttcgca cgcggctgaa cttcgttggt atacgcttct gcttcacgaa tgtattgctg
     7261 ttcgttttca cgcgcggcaa tcgcatcgtc aaacgccgct tttacttctt ccggcggacg
     7321 agcagcctgg aagttgacgt ccagcagagt gatgcccatg tcatacggac gaatcgtctc
     7381 ttccagttcg cgctgggtat cgctacgaat cacggtacga ccttccgtca gaatgcggtc
     7441 catggtgtat ttaccgataa ccccgcgcag ggcgctgtcg gtagcctgac gcaggctgtc
     7501 atccgggctg gtcacgctat acagatattt ttccggattg gtaacgcggt actgcacgtt
     7561 catctcaacg cgcactacgt tctcgtccga cgtcagcatc acaccggaag cggccagttc
     7621 acgcacggct tccacgttta ccggtttgac ttcgtcgata aacgtcggtt tccagttcag
     7681 acccggctca accagatggc tgaatttacc aaagcgtgtt accacgccgc gttcggcttc
     7741 tttaatggta tagaaaccac tggccgccca gataatgaca atcgctgccg ctgcgatggt
     7801 aacgacacga ccgccaagct gcgggcgcgg gccttgcgat gaactgccac cgccagatcc
     7861 ggtgccttta ccgccgccca gaccaccgag ctttttgctc agtttgcgga agatatcatc
     7921 taaatcaggt ggcccttgat cgcgaccgcc tttgtttcca tttccctcag agttgccgcc
     7981 aggtttgctg cttccccacg ggtcgcggtc ttgtccgtta ttaccgggct gattccacgc
     8041 catgtttgtg ctccatattt gttatgcggt gatccctgtt gtctttcgac gccagaggat
     8101 atgactccac gcttcagacg ctacgccgtt agatcaggta atcgatcaac gccggttctt
     8161 gtttacagag gcgacgccag tcaacgatcg gcatacgaac ttgcagactt acgctgccgt
     8221 cctcctccat ccactctttt tctattgcct gaagctgata aaaacgactt ctcagacgcc
     8281 cttcctgcgg tggcagacgc aatgtatgct gcgccacctc gccggaaagc cgctccgtca
     8341 aagcctgaaa aagctgtggt atccccgctc cggtctgtgc ggaaagccag acacggatcg
     8401 gtttgttctc ttcgtcccga tcaatacgcg gttcgaaatc ttccagcata tcgatcttgt
     8461 tcatcaccag cagggttggg atctcgtgag cgtcgatctc ttcaagaacc gtattcaccg
     8521 cttcgatgtt ttcttgtaca cgcacatccg ccgcatcaat gacgtgcagc agtaatgtgg
     8581 cttgccgcgt ctcttgtaac gtggctttaa atgccgccac cagatcgtgc ggcaggtggc
     8641 gaataaaccc tacggtatct gcaagtacgg tttcaccgac atctgcaacg tcaatacgcc
     8701 gcaacgtcgg gtcgagggtg gcaaacaact ggtctgccgc gtagacccgc gcttcggtga
     8761 tgcgattgaa aagggtagat ttaccggcgt tggtatatcc caccagcgaa acagtaggaa
     8821 cgtcggcttt gatacgcgat tgccgcccct gctcacgctg cttttcaact ctttccaggc
     8881 gtgactgtat ctgcacgatg cgattacgca acaaacgacg gtcggtttcg agctgggttt
     8941 cacccggacc acgcaaacct atcccgcctt tctgtctttc aaggtgggtc cagccacgca
     9001 ccaggcgcgt agccagatgg cgcagctgcg ccagctcaac ctgcaactta ccctcatggg
     9061 tacgcgcacg ttgggcgaaa atatctaaaa taaggccggt gcggtcgata acacgacact
     9121 cgcacaaacg ctccaggtta cgctcttgcg ccgggctcag ggcatggtca aaaagaacga
     9181 ccgaagcacc cgtcgctttg acagcttccg caatttcaac tgctttacct tcacctacaa
     9241 aatactttgg gtgcggcgct ttacggctac cggtaatcac ctgcaatgct tcgacaccgg
     9301 cggaagagac cagagattca aactcctgga ggtcttccat atctttgtct tgcgtaaaat
     9361 agatgtgtac cagtaccgcc tgctcaccag catcataacg gtcaaacaag cgtataaccc
     9421 tctaaataga tcagcgggga acgcaggatc gctggctccc cgtgtaaaaa aaacagcccg
     9481 aaaccttatt cggtttcttc gctgtcctgt tgcgcggaag tattctgcgc gctgctacca
     9541 tgatggtagt tactgctggt accgccaccg gcgttgttac tgtgatgaga aaccgggcga
     9601 gacgggacaa cagtagaaat cgcgtgcttg taaaccatct ggctgaccgt gtttttcaac
     9661 aggatcacga actgatcaaa agactcgatt tgcccttgca gcttaatacc attcaccaaa
     9721 taaatagaaa ctggaacacg ttcccgacgc agtgcgttca ggaacggatc ttgtaaagat
     9781 tgccccttag ccattctctc ttttccttat atgcttattt gtactttgaa cctttcgatt
     9841 ctgaaaaaat tgcgcacgat acgtctcaat tgtacacatt cagcctgcga tagcaccaac
     9901 aacctgtaat acttcgtcac gcgcctgttc tggtttttca ctgtcaagcc agtgaacccc
     9961 ttcccaacca cgcagccagg ttatctgccg cttcgccaac tgtctcgtgg cgcaaacacc
    10021 tcgataaacc atttcatcgt atgagatttc gccttcaagg taagaccaca tctggcgata
    10081 acccacgcaa cgaatggaag gcaagtccgt atgcaaatct cctcgggcaa aaagcgcccg
    10141 gacttctgct tcaaaacctg aagccaacat ctgatgaaaa cgctgctcaa tgcgttgatg
    10201 gagcagttca cggctcgccg gggcgatggc gaactgatgc acctgatacg gtagagcgtc
    10261 tcctgacgtt tgcgtcagtt ccgttaaagt tttacccgaa atgaaaaaaa cttccagtgc
    10321 ccgggaaagc ctttgtggat catttggatg aatccttgct gccgcaaccg gatctacctc
    10381 ctgaagttga cgatgcaatg actcccaacc ttgctctgcc gcctgttgct caattctggc
    10441 ccgcacttcc gggtctgccg acggtagcgg cgacaaccct tccagcaatg ccttgaaata
    10501 caacatcgta ccgcccacta acagtgggat ccgccccgcc gcggtgatat cggccatttc
    10561 cgccagcgca tcgcggcgaa aatcagcagc cgagtaagcc tgcgacggat cgcgaatatc
    10621 cagcaatcgg tgcggcgcgg cgagtaactc ttcagcgttc ggcttcgccg tcccgatatc
    10681 catccctttg taaataaggg cagaatcaac gcttatcaac tctactggta aaattttacg
    10741 cagctcaatg gctaacgccg ttttaccgga ggccgtcggc cccatcaaaa aaatcgcctt
    10801 aggcaggctc gccttactga tatcactcat ctttcagggc ttttatcgcc ggatgtaaat
    10861 caacagattg taacagacca cccggcggcg ttttcacaag ttgcggacat aaccgttcca
    10921 cgtccgccag cagggttatg gcctgtgcca ttgaccactg cgcatgttcg ctcatcagat
    10981 ttcgtgcaat ccactgcgca atattgccag gttcgaatac ggactgcttc gccaggtagc
    11041 ctatcagttc aggaatcaag atttgtaaat tttgttggcg taagggtaaa ggcacggccc
    11101 tgatggtcac atgctgtgca tctgactgga aatcaatacc caattccgcc agggcagact
    11161 gcgctttttc taatgccgat ttttcttcgg cagaaacttt tagccgcaac ggaatcagca
    11221 gcggctgggc gcaaacgggc gcttcacccg gcgtcaattg tgcctgacgt agccaacgtt
    11281 ctgccactgg caaggataaa agtgaaatgt tgccatcgcg ctccagcaac gcacagtcgg
    11341 aatggacgat agtcagtacc cgaccaaaac tctgactgtt cgccgcaagt gcaggttcct
    11401 gcggttccgg cgcttttaat ttttgcatcg gcgcgggcgt ttgcaaaagc tggcgataca
    11461 cttcaccttg ctgtttctgg tagcctggct gcgcattcgg ccagggggca gccggacgac
    11521 tgcctgatgc tggcgcagga gtgtagcgcg gagctaccgg ctcacgagct gccggttctg
    11581 caaagtgatt gcgccccgcc gccacgcggt tttccgggat ggcacgcggt gcgggttgtg
    11641 gttcatcgtc cagcggtagc ggcgtttcca gttgctgctg tagcacgctc agcacgccct
    11701 gatagataaa gtcatgcacc agacgcgact gatggaaacg cacttcgtgt ttggcggggt
    11761 gtacgttgac gtccacctga tgcgggtcga tctccagata caacacaaac gccggttgtt
    11821 gatcggcccc cagtttgtct tcgcaggcct ggcggatcgc atgattgatc aggcgatcgc
    11881 gcatcatgcg accattcacg tagcaatact gaatttccgc cagtgcgggc gtagtgtgat
    11941 ttggatcggc cacccagccg cgcagggtga gatcgccgtg ttgccattca atcgccagcg
    12001 cttgttcgag aaaagcggtg ccgcaaatcg cgcctaagcg ccgttctttt tgcccgcctt
    12061 ccggcactgc gcggtactga cgcacaattt taccgttatg cgacaggttg atcgtgacgt
    12121 cgaaacgcgc cagcgcaatg cggcggatga tctcatcaat gtggttaaat tcggttttct
    12181 cggtgcgcag gaatttgcgc cgcgccgggg tgttgtagaa cagatccagc acctccagcg
    12241 tcgtccccac aggatgcgcc gccggtttta ccgtcacgtt catatcgcgc ccttcggcat
    12301 aggcctgcca ggcttcctgc tgttctgcgg tgcgtgaagt gagcgtcagg cgggaaaccg
    12361 aactgatact cgccagcgcc tcaccgcgaa agcccaggct gataatggct tcgagatcgt
    12421 ccagagaggc gattttactg gtggcatgac gcgccagcgc cagcgccagc tcgtcttttt
    12481 tgataccgca gccgttatca cgaatgcgga taagtttcgc cccaccgcgt tcgatatcaa
    12541 tatcgatacg tgtcgcacct gcatcgaggc tgttttccac cagttctttg actaccgacg
    12601 caggtcgctc gaccacctca cctgcggcaa tctggttcgc cagttgtggc ggtaagacct
    12661 gaattggcat caatcgtcct tagtttggca gcgtgcgatc tggcgtcgtc accgtactgg
    12721 ccgtttgtgc cgttgccccc tgcggcgcag attgcatcgg atgcgcaagg aaataattgc
    12781 gcaggccttt gtaaatggct tctgccagct gttgttggta atcgtcgctc gccagcaaac
    12841 gttcttcgct gttgttgctg ataaaaccgg tttcgaccag tactgatggg atatccggcg
    12901 aacgcagaac gccgagactc gcgtgttctg gtcggcgttt atgtatttcg ccaatgcgtt
    12961 gcaactgact gatcatactg gtcgctacat cataccctac ccgttgggaa tgaccgaact
    13021 gtaaatccag caccgcctgg cttaaatagg ggtcagactg actgttcgcc agcacatcac
    13081 ccgccccacc cagcagctcc gactgtttct cgtgctgctc cagccagctg gccatttcgc
    13141 tgttggcgcg acggttagag agcacccata cggaagcgcc agtcgcactg cggttcggtg
    13201 cggcatcagc gtgaatcgac acgaggaaat tggcgttttg cttacgtgcc acatcactgc
    13261 gccccatcac cgagataaag taatccccgt cacgggttaa aacgccctta aacatcggat
    13321 cgtcattgag caaagtacgc aatttacgcg cgatggcgat ggtgacattt ttctcccgcg
    13381 taccaccggg gccgatagcg ccagggtcct gaccgccgtg tccggcatca atagcgatga
    13441 taattttatc gccagtgtta gccgtcgcgc gcgctgccgg acgcgttacc gtattactgc
    13501 tgataacacc cgtagtgcgg ttactttccg ttttaaacgg attgcgcgcc ggttcgctga
    13561 cgcgcggtgc gacaaccgca ggcgtttcaa cgcgtttcgc aaccacgggc ggcggaggag
    13621 gaggtggcgg cgcatcggcg ttaatcgtaa agacgacagt gtaattgctg ccattctgcc
    13681 gcttcaccgc ttcggtttta ccgttttcgg taagatcgac caccagccgt agcgtttgtg
    13741 catctttagg cgttccagag cgaatcgcct tcaccagatt attgccgctg aacaacaacg
    13801 gcagtccctg aatcacgccc gtttgtttga tatcgagcgc cacggtgcgt ttgctttgat
    13861 ggctaaacgc ataatcagga tcgccaataa aactcaacgt tatccgcgcc tgttgattac
    13921 cgttagaaac ctgaatatca gagagcgtcg cggcacccac cggcgtgcac agcagcagca
    13981 gcgtcgctac caaccaattt ctgatgcgat acatcatccc gccacctttc aaaggttaac
    14041 cggctaaacg cgccagcaac aattcacccg cagaggaaac cgcactcacg cgcgcctcac
    14101 ggccttgtgc ctgataatca atgtgtattt cgacatccgg gtcaggaaga acacctgtac
    14161 cttgttgtgg ccactccacc aggcagatgg catcgttggc aaaataatcg cggatcccca
    14221 taaactccag ctcctcggga tcggcaaggc ggtacaaatc aaagtgataa accattaaat
    14281 tgtcgagcgt atagggttcg accagcgtat aagtggggct tttgacatta ccctgatgac
    14341 ccagagcttg taaaaagccc cggctaaagg tggttttacc tgcgcctaaa tcgccataca
    14401 gatagattac ggttgcgcca tcgcaggctt tcgctacccg ctcgcccagg tctaatgttg
    14461 cctgctcatc agggagcgga attactcgat tcatcatggt ttttatcagt cacttccggg
    14521 ttaacaatac gctgtagcgt ggaaaagaga tcggttgcca gcatcccgcg cgttccaaaa
    14581 cgcgccgcca gtacgtcagc tgcagcaccg tgcgcgacac agcccgcaca ggcggcatca
    14641 tacggcgaca gtttttgccc aagcaatgcg ccaataatac cagagagcac atcgcccatg
    14701 ccgccgctcg ccatgcctgc atttccggca tcaataatgc ctaaagcgtc aggatgggcg
    14761 gcgaccacgg ttccggcacc tttcagcacc gctacgccgc cataacgttg taccagacgt
    14821 ttggcgcaat gtaagcggtc actttcaatt tcagcgacgg aacagcctaa caaccgtgcg
    14881 gcctcgccag gatgcggcgt gatcacgcga ttgtgacgct tatcgggatt gattgccagc
    14941 aggttcaatg catcggcatc ccacaacatc ggtttgcgaa aattctcaac tttttgcagt
    15001 gcttttttcc cccactcttg ctggcccaga ccgggaccaa tcaccaccac atcggcccat
    15061 tccaggcttt cggtaagaga gtccatcgtc agttcatgca ccatcaattc cggtcgtgca
    15121 gtcagcagcg gcgcaatgtt ttcactgcgg gtcagtactc ggactaaacc agcaccagca
    15181 cgtagcgccg cttcccccgt catacgaata gcccccgccg tgccgtgatc gccaccgata
    15241 attaccagcc gcccgtgatc gcctttatgc gaagtcgggc gacgcggttt tagccagtga
    15301 gaaagttgtt ctgccgaaaa ccgctgaatt ttcgtctcct gacctgccag ccaactatcc
    15361 agccccagtg agtcaaaatg cagttgtccg gtaacatccc gcgcttttcc agtgagcaag
    15421 cctggtttca gcgcaataaa agtgatggtg tgatcggcgt tgatcactgc gcctggcgta
    15481 gcgccggttt cagccagcag gccggaaggg atatcaaccg ccgcaatcgg cgcaggatgg
    15541 gaattagcgt ggtcgattaa ctggctaatg gattcgcggg gcgcttgctg caagccggta
    15601 ccgagcagcg catcaacaat cagatctacc gattcgggcc agacaatatt cgaagcatgg
    15661 atctcgcctc ccgcgtttaa ccatgcttcg cgtgccagcg cggcctcttc cggcaacggt
    15721 ttgtcgctct cctgggccag cagcgtgacc tcaatgccga ccgctgtggc cagccgcgcg
    15781 accacgtagc catcgccgcc gttattacca tgaccgcaca gcaccagcca atggcgggcg
    15841 tcaggatacg ccgaacgaca cacctggaat gcggcttcgc cagcgcgaag catcagctca
    15901 tagagtgtaa gccccagcgc atctgccgcc tcacgttctc cgcggcggat atcgtcggcg
    15961 tgccagacgg tgtgtggtat acttacgggg tttttcttca ttgtatggtc cgtcatgtca
    16021 gagcccctcg atctcaatca gttagcgcaa aaaattaaac agtgggggct ggaactgggc
    16081 tttcagcagg taggtattac cgataccgat ctcagcgagt ccgagcccaa actgcaagca
    16141 tggctggaca aacaatacca cggcgaaatg gactggatgg cacgtcacgg tatgctgcgc
    16201 gctcgccccc atgagttatt gcccggtacg ctgcgcgtga tcagcgtgcg gatgaattac
    16261 cttcctgcta acgccgcatt tgccagcacg ctgaaaaacc ccaaactcgg ctatgttagc
    16321 cgttatgcgc tgggccgtga ctatcacaaa cttctgcgca accgactcaa aaagctgggc
    16381 gagatgattc agcaacattg tgtttcgctg aattttagac cgtttgtcga ttctgcgcct
    16441 attctcgagc gcccgttagc tgaaaaagct gggctcggct ggacaggtaa gcactcactt
    16501 atcctcaatc gcgaggccgg ttctttcttc tttttaggcg aattgctggt cgatattccg
    16561 ctgcccgtgg atcaaccagt cgaggaagga tgcggtaaat gcgtggcctg tatgacgatt
    16621 tgcccgaccg gtgccatcgt cgagccatat accgtcgatg ctcgccgctg tatctcttat
    16681 ctcaccatcg aacttgaagg ggcgatcccg gaagagttgc gaccgttaat gggaaaccgt
    16741 atttacggtt gcgatgactg ccagcttatc tgcccgtgga atcgctattc acaactcacc
    16801 acagaagagg atttcagccc gcgtaagccg ctacacgcac cggaactcat tgagttattc
    16861 gcctggagcg aagagaagtt tttaaaagtc acggaaggat cggcgattcg tcgtattggt
    16921 cacctgcgtt ggctgcgtaa tatcgccgta gcattaggca atgccccttg ggatgaaacg
    16981 attttgacag cgctggaaag tcgtaaaggt gagcacccac ttctcgatga gcacatagcg
    17041 tgggcgattg cgcagcaaat agagagacga aatgcgtgca tagtcgaagt gcaattgccg
    17101 aaaaaacagc gtctggttcg ggtgattgaa aaagggttac cgcgtgacgc ctgattcatc
    17161 cacagcttgt gaataaaaat aaaaacgcat tgcaattcaa gacgaataaa atcgtcaagt
    17221 gatcgactcg acaaaacgga atcttaattt ttaacttatc tatcaatagg ttaaaaaaat
    17281 actattcacc gtgcgaagtt tctttgaaat taagattaca gcctgaggct gtggataact
    17341 ctgtttacaa gagtttgtca gagacaacga aaaccagcaa taacgcatcg catcgctgtg
    17401 gatattttat tgagagaaga atttggagcg ggaaacgaga ctcgaactcg cgaccccgac
    17461 cttggcaagg tcgtgctcta ccaactgagc tattcccgcg tactacttaa ttttgctttc
    17521 gtaattttcg catttctgcg tcgttacggg aggcgcatta tacgagaaat ctttccctct
    17581 gcaacccccc aaaaaagttt tttttgcgtt tttatctcaa gtgctgaaat tatcagcaag
    17641 acgttcaatt tagcgtcaaa atcacgccgc cgcaagtgga ttccggcgct tacgtgacca
    17701 ggaaaaattt tacagcttga taaaatgctc gcggtagtaa gccagctccg ccaccgattc
    17761 acggatatca tccatcgcct gatgcgtccc ctgcttggta aaaccatcca gaatttccgg
    17821 cttccagcgg cgcgccagct ctttcagggt gctgacatcg agataacggt agtggaagta
    17881 ggcttccagc tccggcatgt atttaaacag gaaacgacgg tcctgaccga tgctgttacc
    17941 gcaaatcggc gattttcccg caggcaccca ctgttttaaa aattcgagcg ttgccagttc
    18001 agcttcccga tcgcccatcg tgctcgcttt cacgcgctct accagcccgc tggcggtatg
    18061 ggtgcgcacg ttccagtcat ccatcagcgc cagctgttca tcagactggt gtactgcaat
    18121 ggtcggccct tctgccagaa tattcaggtt ggcatcggtc accagcgtgg caatctcaat
    18181 aatgcgatcg cgctcgggat ccagaccggt catctcaaga tcgatccaaa tcaggttgtt
    18241 ttcattggca ctcatgctat tttccaccca ttcacagtga ctatattgat ccacaattgc
    18301 gtgtatcata gatgttttgc ccatcagggg cgaccaggag tctgtacgat tgagtaaaaa
    18361 taaactctcc aaaggccagc agcgccgcgt gaacgccaat caccagcgtc gtcttaaaac
    18421 gtctaaggag aagcccgact acgacgacaa tctgtttggc gagcctgatg aaggtatcgt
    18481 catcagccgc tttggtatgc acgctgatgt ggaatccgcc gatggcgacg ttcaccgctg
    18541 caatattcgc cgtaccatcc gttcgctggt aaccggcgac cgcgtagtct ggcgtccggg
    18601 taaaccggcg gcggaaggcg taaatgtcaa agggatcgtg gaagcggtgc atgagcgtac
    18661 ctcggtgttg acgcgtccgg atttctacga cggcgtaaaa cctattgccg ccaacatcga
    18721 ccagattgtc attgtctccg ccattttgcc ggagctgtcg ctcaatatta tcgaccgtta
    18781 cctggtggcc tgcgaaacct tgcagattga gccgattatt gtgctcaaca agatagacct
    18841 gctggacgac gaaggcatgg cgttcgtcaa cgagcagatg gatatctacc gcaatatcgg
    18901 ttatcgcgta ttgatggttt ccagccatac tcaggatggg ctaaaaccgc tggaagaggc
    18961 gttgaccggg cgcatcagca tttttgccgg gcagtctggc gtcggcaaat ccagcctgct
    19021 gaatgcgttg ctggggctgc aaaaagagat cctgaccaac gatgtctctg acaactcggg
    19081 gctcggtcag cacaccacga ccgccgctcg gctgtatcac ttcccgcacg gcggtgatgt
    19141 gattgattcc cctggtgtgc gtgagttcgg cctctggcac ctggagccgg aacaaatcac
    19201 tcagggcttt gtcgaattcc atgactactt aggtctgtgt aaatatcgcg attgcaaaca
    19261 cgataccgat ccgggctgcg ctatccggga agcggttgag gaagggaaaa tcgcggaaac
    19321 ccgtttcgaa aactatcacc gtattctgga aagcatggcg caggtaaaaa cgcgtaaaaa
    19381 cttttctgat acggatgact gacaattaag ctaaccgtcg ttagaatcgt cccctttttt
    19441 tcaggatccg gcatgtaggc cggatcagga acgacaaaac aatggcctgg aggctacctt
    19501 gttaaattca tttaaacttt cgctacagta cattctgccg aaactatggc ttactcgcct
    19561 ggcgggttgg ggcgcaagca agcgggcagg atggctgaca aaactggtta tcgatctgtt
    19621 cgttaaatac tacaaggtcg acatgaaaga ggcgcaaaag ccggacaccg ccagctaccg
    19681 cacctttaac gaattctttg tccgtccgct gcgtgacgaa gtacgcccaa tcgataccga
    19741 tccgaatgtc ctggtcatgc ctgccgatgg cgttatcagc cagttgggta aaatcgaaga
    19801 agataaaatc ctgcaagcca aaggccacaa ctacagcctc gaagccttgc tggcaggcaa
    19861 ctatctgatg gcggacctgt tccgcaacgg tacgtttgtg accacttacc tctccccgcg
    19921 tgactaccac cgcgtacaca tgccgtgcaa cggtattctg cgtgagatga tctacgtgcc
    19981 gggcgatctc ttctccgtta accatctcac cgctcagaac gtgccgaatc tgtttgcccg
    20041 taacgaacgc gtgatttgcc ttttcgatac cgaatttggc ccaatggcgc agattctggt
    20101 cggagcgacg attgttggca gcattgagac ggtctgggcg ggcaccatta cgccgccgcg
    20161 cgaaggtatc atcaagcgct ggacctggcc tgccggggaa aacgacggtt ctgtggcact
    20221 gctgaaaggc caggaaatgg gtcgctttaa actcggttcc accgttatca acctgtttgc
    20281 accgggtaaa gtgaatctgg ttgagcaact ggaaagcctg tctgtcacga aaattggcca
    20341 gccgctggca gtatctaccg aaacctttgt tacgccagac gctgaacctg ccccgcttcc
    20401 tgctgaagag atcgaagcag aacacgacgc cagcccattg gttgacgaca aaaaagacca
    20461 ggtctaatcc atcaaaggaa acgctgacgt gcgcctgatt atcacttttc tgatggcctg
    20521 gtgcctcagt tggggggcgt acgccgcgac ggcccccgat agcaaacaaa tcactcagga
    20581 actggagcag gcaaaagcgg cgaaacccgc acagccggaa gtcgtagagg cgctccagtc
    20641 tgccttaaat gcgcttgagg aacgaaaagg ttcccttgag cgcatcaaac aatatcagca
    20701 agttatcgat aattatccga aactctccgc tactctgcgc gcacaattaa acaacatgcg
    20761 tgacgagccg cgcagcgtgt cgccgggaat gtctaccgac gcgctgaatc aggaaattct
    20821 ccaggtcagc agccagttgc tggataaaag ccgtcaggcc cagcaagagc aggagcgcgc
    20881 ccgcgagatt gccgattcgc tgaatcaact gccgcaacag caaaccgacg cccgccgtca
    20941 gttaaatgag atcgagcgcc gcctggggac gcttactggc aatactccgc tcaatcaggc
    21001 acaaaatttc gcgttgcagt ctgactctgc acgtcttaag gcgctcgttg atgaactgga
    21061 gctggcgcag ctgtctgcca ataaccgcca ggaattagcg cgcttacgct cagagctggc
    21121 ggaaaaagag agccagcaac tggatgcgta tttgcaggcc ttgcgtaatc aattaaacag
    21181 ccaacgtcag cttgaggcgg agcgggcgct ggaaagtacc gaattactgg cagaaaacag
    21241 tgccgatttg ccgaaagata tcgtcgcgca attcaaaatt aaccgcgaac tatcggcggc
    21301 tttgaatcaa caggcgcagc ggatggatct cgttgcctcg caacagcgtc aggctgccag
    21361 ccagacgtta caggtccggc aggcgttgaa tacgctgcgt gaacagtcgc aatggctggg
    21421 atcgtccaat ctgctcggcg aagcgctgcg ggcgcaggtg gcacggctgc cagaaatgcc
    21481 aaaaccacaa cagcttgata ccgaaatggc gcagttgcgt gtgcaacggt tacgttatga
    21541 ggatctgctt aataaacagc cgctgctacg gcaaatccat caggccgacg gtcagccgct
    21601 gaccgccgag caaaaccgta ttctggaagc acaactgcgc actcagcgtg agttgctgaa
    21661 ctcattgttg cagggtggcg acacgctact attggaactg accaagctga aagtctccaa
    21721 cgggcaactg gaggatgcgc tgaaagaggt gaacgaagca acgcaccgct atctgttctg
    21781 gacctctgac gtgcgcccga tgaccatcgc ctggccgctg gaaatcgccc aggatctgcg
    21841 tcgtctcatt tcgctggaca ccttcagtca gttgggcaaa gccagtgtga tgatgctgac
    21901 cagcaaagag acaattttgc cgctgtttgg cgcgttgatt ctggtcggtt gcagtattta
    21961 ctcgcgccgc tatttcaccc gttttcttga acgttcggcg gcgaaagttg gcaaagtgac
    22021 tcaggatcac ttctggctga cgttgcgcac tcttttctgg tcgattctcg tcgcgtcacc
    22081 gttaccggtg ctgtggatga cgctgggtta cggcttgcgc gaggcgtggc cttatccgct
    22141 ggcggtcgcg attggcgatg gtgtaacggc caccgtgccg ctgctgtggg tagtgatgat
    22201 ttgcgccacc tttgcccgcc cgaacggctt gtttatcgct cattttggct ggccgcgcga
    22261 acgtgtttcc cgtgggatgc gctactacct gatgagcatc gggcttattg tgccgctgat
    22321 tatggcgctg atgatgttcg ataacctcga cgaccgtgaa ttctccggtt cgctgggacg
    22381 gctttgcttt atcctcattt gcggtgcgct ggcggtggtc accctcagcc tgaaaaaggc
    22441 cgggatccca ctgtatctca acaaagaggg cagcggcgac aacattacca accatatgct
    22501 gtggaacatg atgattggcg cgccactggt tgccattctg gcgtcggcgg tgggttatct
    22561 ggcaacggca caggcgctgt tagcgaggct tgaaacctcg gttgccatct ggttcctgct
    22621 actggtggtt tatcacgtta tccgccgctg gatgctgatc caacgccgca ggctggcgtt
    22681 tgaccgggcg aagcatcgcc gggcagagat gttagcgcaa cgtgcgcgtg gcgaagagga
    22741 agcacatcat cacagtagcc cggaaggggc aattgaagtc gatgaaagcg aagtcgatct
    22801 cgatgccatc agtgcgcaat ccttgcggct ggtgcgctca attttgatgt tgatcgccct
    22861 gctttcggtc attgtgctgt ggtcagaaat ccattccgct ttcggcttcc tcgaaaatat
    22921 ttcgctgtgg gatgtcacct ccacggtaca gggcgtagaa agtctggagc caattaccct
    22981 cggtgcggtg ctgattgcca ttctggtgtt tatcatcacc acgcagctgg tgcgcaacct
    23041 gcccgcgctg ctggaactgg cgattttgca gcacctggat ttaacgccgg gtacgggtta
    23101 cgccatcacc accatcacca aatatctgct gatgctgatt ggcgggctgg tcggcttctc
    23161 aatgattggt attgagtggt cgaaattgca gtggctggtt gccgcgctcg gtgttggtct
    23221 cggttttggt ttacaggaaa ttttcgccaa ctttatctct ggcctgatca tcctgttcga
    23281 aaaaccgatt cgcattggcg atacggtaac gatccgcgat cttactggta gcgtgacgaa
    23341 aattaatacc cgcgcaacca ccatcagcga ctgggaccgt aaagagataa tcgtgccgaa
    23401 caaggcgttt attaccgagc agtttatcaa ctggtcgctc tctgactcgg tcacgcgcgt
    23461 ggtgttgacg attccggccc ctgccgatgc caacagtgaa gaagtgacgg aaatcctgct
    23521 caccgcagcg cgtcgctgct cgctggtgat cgacaacccg gcaccggaag tcttcctggt
    23581 ggatctgcaa caagggattc agattttcga gctgcgtatt tacgccgctg agatgggtca
    23641 ccgtatgccg ctacgccatg agatccacca gctgattctg gcaggcttcc atgcccacgg
    23701 tatcgatatg ccattcccgc ccttccagat gcgtctggaa agcctgaatg gtaaacaaac
    23761 ggggagaaca ttaacttctg cgggcaaagg tcgtcaggcg ggaagtttgt aatgcatgaa
    23821 gctgccaaat gatgtcaacg tggctcattt agcagcttaa catcatcttc agttatcacc
    23881 ggccctggag agtatcatcc atacgcatta cttcctgaca actcatcgcc ggagagacga
    23941 tgagttgcag gatttcatat gatcagtcga gagctaactc aggtttattc gccatgcgac
    24001 tgcaatagtt ctggtaaatc gccatcgcta acagcgagaa gaagacaggg ccgccaatca
    24061 tccacaatgt gctgtcccag tctccagctt caaccacagg ttgaatgatg gtgaagacgt
    24121 tcgcaaatgt caccaccagt acgacaacca ccgtcgcgat cattgccgac atacgcgttt
    24181 tgaaaatcac aaatggtctg tcgagatcct gacgtgcttt aaagaatggg aaagccagcg
    24241 cgaggaacag gtaaggaagc gtcatagaca cgttcgccat cagcgtcagc ttgttaaaga
    24301 acgccgatgc ggtaccgcca ccaaacgaaa ccagcagaat gaagatagta accaacccgc
    24361 actgcatcca catggagata gacggcatcc ccatcgcatt caggcgcgtc atcggttccg
    24421 gccacaatgc tttcggcgtc ccctggatga tggctttcaa cggtgaatag caaagcgtaa
    24481 agaacgcacc ggtataggcg aggaacatcg aaagtccagt aatacgcgca aaccatacgc
    24541 ccagcgacaa tgacgcttca ggtgacaaat gcagtgcatt acccagcgtc atcccaaggc
    24601 tcttcatcag cacataggta atattgccga ggttaacgga accattactt aatacctgct
    24661 gccagtttgt gctgacgccc cataaaaata ttgccagcga ataaccgatt gaaataacaa
    24721 tagcggcaaa aacaatacct ttggcaaagt tcttttctgg attttccgtt ttatcgacca
    24781 gaccaccaac cgcttcaatt ccgccatagg caaaaatggc aaataccaca aacgatagca
    24841 ttgccagacc ggactgataa cccgggttcg gtgatgcaag gaaattaata tcctgcgcga
    24901 aatgcccacc atttaataac aaaatagtaa tgcttactaa cagcaatact aaattcagac
    24961 acattactgc aataccgccc accgcagtaa tgcgggcaat tttattaatc cccttagaag
    25021 caacgacggt gaccagaatc atccatgcca ctgccagcag accaaccacc tgcgtaggct
    25081 ccagtccggc aatacgccag tgctgggtca tgtcgctacc atagaggaat gttgagaacg
    25141 gtacccaaac tttcgcggag gtactcacca tccagatgat ataagaggaa aaccacataa
    25201 acgtaccaat gaaggcaaaa cgtggtccga cactattatt catccaggaa tagataccac
    25261 cttcttcttt gcgataagca gctcccattt cagccatcat taaggcgaat ggaataaaga
    25321 ataacaatgc agaaaatata taaaagggaa tcgcactata acccattaag taataagccg
    25381 atgggctatt ggcaaaacca aatacggaag taaagatcat caatatgagt cctatcagac
    25441 tcattttttt tatcgtgtga ggcatgaaac catccttcat ttcagcgcgt gtataacacg
    25501 tgcccactga actgcactgc gcaacattga gcaggcagcg taaaaaccaa tacagaattg
    25561 atacctggat gatatcaaat atccagtctt aaattgtggc ctttagcgga aaaatgaaaa
    25621 catatgctac agaatggcgc ttattacgcc attcttcgct gttaattcag taatttttca
    25681 gaattatgcc cggtcaacgc taaaggcgat gacttcagcc agtgtctccg cgcccagcgc
    25741 caacatcacc agacgatcaa cacctaatgc cacgccggaa cagtcaggca taccgacttt
    25801 caaggcgtca atcagattct ggtcaatggg gtgctgcggc aaaccgcgcg ccgcgcgctt
    25861 acggttatct tgttcaaagc gttgttgctg ctcacgggca tccgtcaatt catggaaacc
    25921 attcgccagc tcaatacctt tataataaac ctcaaagcgt tcagcgaccc gatgatcttc
    25981 ggtactgatt tgcgccagtg atgcctggct ggctggaaag tggtacacaa aggtcggttt
    26041 ttctttacca atatttggct ctacgccaaa ggtaaacagc aattgtagca acgtgtcgcg
    26101 gtcttcttcg gtatcagcaa cattgctcaa atccagtttc gctgccactt cccgcagttg
    26161 cgttttgtcg gcagagagtg ggtcaatttc cagataacgc aagaaagctt gttgataaga
    26221 aaggctttct gctgccgggc agtccagcac ctgttgtaag agatcgtcca cctcgttcat
    26281 caaccggtac atatcatagt gcggtcgata ccactccagc atagtgaact cagggttgtg
    26341 ataacgcccc atctcttcat tacggaagct gcggcacagc tggaataccg gcccacaacc
    26401 ggcaaccagc aggcgtttca tatggtattc cgggctggtc attaaccaga gattcatccc
    26461 ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg accaaatgaa tatcggttac
    26521 cgtcgcctgg ctcatacaag gcgtctccac ctccagcact ccacgatcgg caaagaaacg
    26581 acggatctcc gccataatcg ccgcgcgttt taataagtta ggaatggatg cgctcggctg
    26641 ccaggatgcc gtttcgctca tagttaaatc tccagttttt gacaagggca cgaagtctac
    26701 tcgcaacgcg acggcgagac aaattttacg caggaatcaa acagcggtgg gcagtgacta
    26761 aaaaaagcac gatctgatgg tttagtaatt aaattaatca tcttcagtga taatttagcc
    26821 ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc agactatact
    26881 gttgtaccta taaaggagca gtggaatagc gttcgcagac cgtaactttc aggtacttac
    26941 cctgaagtac gtggctgtgg gataaaaaca atctggagga atgtcgtgca aacctttcaa
    27001 gccgatcttg ccattgtagg cgccggtggc gcgggattac gtgctgcaat tgctgccgcg
    27061 caggcaaatc cgaatgcaaa aatcgcacta atctcaaaag tatacccgat gcgtagccat
    27121 accgttgctg cagaaggggg ctccgccgct gtcgcgcagg atcatgacag cttcgaatat
    27181 cactttcacg atacagtagc gggtggcgac tggttgtgtg agcaggatgt cgtggattat
    27241 ttcgtccacc actgcccaac cgaaatgacc caactggaac tgtggggatg cccatggagc
    27301 cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat cgagcgcacc
    27361 tggttcgccg ccgataagac cggcttccat atgctgcaca cgctgttcca gacctctctg
    27421 caattcccgc agatccagcg ttttgacgaa catttcgtgc tggatattct ggttgatgat
    27481 ggtcatgttc gcggcctggt agcaatgaac atgatggaag gcacgctggt gcagatccgt
    27541 gctaacgcgg tcgttatggc tactggcggt gcgggtcgcg tttatcgtta caacaccaac
    27601 ggcggcatcg ttaccggtga cggtatgggt atggcgctaa gccacggcgt tccgctgcgt
    27661 gacatggaat tcgttcagta tcacccaacc ggtctgccag gttccggtat cctgatgacc
    27721 gaaggctgcc gcggtgaagg cggtattctg gtcaacaaaa atggctaccg ttatctgcaa
    27781 gattacggca tgggcccgga aactccgctg ggcgagccga aaaacaaata tatggaactg
    27841 ggtccacgcg acaaagtttc tcaggccttc tggcacgaat ggcgtaaagg caacaccatc
    27901 tccacgccgc gtggcgatgt ggtttatctc gacctgcgtc acctcggcga gaaaaaactg
    27961 catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt cgatccggtt
    28021 aaagaaccga ttccggtacg tccgaccgca cactacacca tgggcggtat cgaaaccgat
    28081 cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc ctctgttggt
    28141 ctgcacggtg caaaccgtct gggctccaac tccctggcgg aactggtggt cttcggccgt
    28201 ctggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa cgaagcggca
    28261 attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctggttaa ccaggatggc
    28321 ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg caatggaaga aggttgcggt
    28381 atctaccgta cgccggaact gatgcagaaa accatcgaca agctggcaga gctgcaggaa
    28441 cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt tcaacaccga cctgctctac
    28501 accattgaac tgggccacgg tctgaacgtt gctgaatgta tggcgcactc cgcaatggca
    28561 cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccga gcgtgacgac
    28621 gtcaacttcc tcaaacacac cctcgccttc cgcgatgctg atggcacgac tcgcctggag
    28681 tacagcgacg tgaagattac tacgctgccg ccagctaaac gcgtttacgg tggcgaagcg
    28741 gatgcagccg ataaggcgga agcagccaat aagaaggaga aggcgaatgg ctgagatgaa
    28801 aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac cgcatagcgc
    28861 attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg gctacatcaa
    28921 agacaacctg gcaccggacc tgagttaccg ctggtcctgc cgtatggcga tttgtggttc
    28981 ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta
    29041 caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacgcg atctggtggt
    29101 cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca tcggcaactc
    29161 ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcaa agtatcacca
    29221 gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa
    29281 cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg aagatagccg
    29341 cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggcg tatggagctg
    29401 tactttcgtg ggctattgct ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat
    29461 tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga aaccacgcta
    29521 aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg tccacctggt
    29581 ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg gttccggctg
    29641 tgtggttcag cattgaactg attttcgggc tgtttgccct gaaaaatggc ccggaagcct
    29701 gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt gatcattaac ctgatcactc
    29761 tggcggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa gcggccaata
    29821 tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc tgggcggtaa
    29881 ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga gcctgagatg
    29941 attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt cggggccggt
    30001 ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat tctgctgcca
    30061 ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt cgcgcagagc
    30121 ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg tggtttacac
    30181 cgtatgcacc acgcgatgca cgatctgaaa attcacgtgc ctgcgggcaa atgggttttc
    30241 tacggtctgg ctgctatcct gacagttgtc acgctgattg gtatcgttac aatctaacgt
    30301 atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa ccagacctta
    30361 tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttgctccaca tttgctgccc
    30421 ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag caacaaaaga
    30481 tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt acctggggct
    30541 atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag ttaggttcgg
    30601 tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt
    30661 taagcgatcc cgcaacaaaa tactggcctg aacttaccgc taaacagtgg aatgggatca
    30721 cactattaca tcttgcgacc tacaccgctg gcggcctgcc attgcaggtg ccggatgaag
    30781 tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca tgggctccag
    30841 gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg gctgtgaagc
    30901 cgtctggttt gagttttgag caggcgatgc aaactcgtgt cttccagcca ctcaaactca
    30961 accatacgtg gattaatgta cctcccccag aagaaaagaa ttacgcctgg ggatatcgcg
    31021 aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc tgaagcttat ggtgtgaagt
    31081 cgaccattga agatatggcc cgctgggtac gaagcaatat gaatccccgt gatatcaacg
    31141 acaaaacact tcagcaaggg atacaactgg cacaatctcg ctactggcaa accggcgata
    31201 tgtatcaggg cctgggctgg gaaatgctgg actggccggt aaatcctgac agcatcatta
    31261 acggcagtgg caataaaatt gcactggcag cacaccctgt aaaagcgatt acgcccccaa
    31321 ctcctgcagt acgcgcatca tgggtacata aaacaggggc aaccggcgga tttggtagct
    31381 atgtcgcgtt tattccagaa aaagagctgg gtatagtgat gctggctaac aaaaactatc
    31441 ccaatccagc gagagtcgcc gccgcctggc agattctcaa cgctctacag taaaatttca
    31501 tcgggtccga attttcggac cttttctgcg cttttccttg ctgtcatcta cacttagaaa
    31561 aaaaccagta aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc gacagctgca
    31621 tttctggtcg ttgcctgcag ttctcctacg ccgccgcgtg gcgtaacagt agtgaataat
    31681 ttcgacgcaa aacgttatct cggtacctgg tatgagattg cccgttttga tcaccgtttt
    31741 gaacgtggac tggaaaaagt caccgcaaca tacagtctgc gtgatgacgg cggcctgaat
    31801 gtcatcaata aaggctataa ccctgacaga gaaatgtggc agcagagtga agggaaagcg
    31861 tactttaccg gcgcaccaac acgcgctgcg ctgaaagtgt cattctttgg tcctttctat
    31921 ggcggttata acgtcattgc acttgatcgg gaataccgcc atgcgctggt ttgcggcccg
    31981 gaccgcgact acctgtggat actctcccgc acgccaacca tttctgacga agtgaaacag
    32041 gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca gtaagtttat ttgggtacag
    32101 cagcccggta gttagtgagt gctgagtttc agaccaataa tccccaatac gattaacgcc
    32161 agactcgcca ggcgcatcgg gttagcggac tcaccgagca gcacaatgcc ggtgatggcc
    32221 gcgccgactg cgccaatacc cgtccacacg gcataagccg tccctaccgg taacgatttc
    32281 atcgcccagg caagtagcgc catactgact atcatcgccg tcacggtaat aacactcggc
    32341 gtcaaacggc taaagccgtg ggtatatttc aggccaacgg cccataccac ttccagcaga
    32401 ccagcaataa ctaagataat ccaggacata tcaggctccg gagaattggg gccgtccccg
    32461 gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga tattttgcta
    32521 ggtggcaggg gaatttcaac tattaccttt gtgactatcg caaaaaaaca aacggcacga
    32581 cataactgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcacc agaaatcgcg
    32641 ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc agttaatact
    32701 gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat aacgtcttcc
    32761 ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa catcgccaaa
    32821 aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca gcgcgggaga
    32881 tggagttgcc gagatgtttg atgtcttcgc cgaaaccgcg agcggtgtta cagcccgtga
    32941 gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct gacctgaata
    33001 agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga gagacgtatt
    33061 caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc ggaactttaa
    33121 ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta tcacctttca
    33181 ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta acggagatcg
    33241 gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat ttagcgttgt
    33301 cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc atgaagtgcc
    33361 agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca gcgccttcag
    33421 cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga cgacgcagtt
    33481 taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct tcaaccgcgt
    33541 aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta tagtacgttg
    33601 ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc gcatattgta
    33661 accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt tgtgaccgat
    33721 cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt ggccggacgc
    33781 agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg catggagaaa
    33841 ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga gtttgtcgtc
    33901 gcgtccggat tctccaccga cccgctggaa gaacagcaca gcgtagtgcc tggtttgttg
    33961 cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt aaattgccgc
    34021 tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg taactggcag
    34081 actgctcttg agtatgttgc tgcgcatccg gaactggacg agatgatttt ctccggcggc
    34141 gatccgctga tggcgaaaga tcacgagctg gactggttgc tcacacaact ggaagccatc
    34201 ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc ggcacgtatc
    34261 accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct ggtgaatcac
    34321 atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa attgcgccgt
    34381 gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga taacgcacaa
    34441 acgctggcaa acctgagtaa tgcgttgttc gatgccggcg taatgccgta ttacctgcat
    34501 gtgctcgata aagtacaggg cgcggcgcat tttatggtga gtgatgacga agcacggcag
    34561 attatgcgtg agttgctgac actggtgtcg ggatatctgg tgccgaaact ggcgcgagaa
    34621 attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca gtaactgatt
    34681 aaacagggaa gtcaaacatt aaaattgcct gatgcgctac gcttatcagg cctacatcgt
    34741 gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt tacgccgcat
    34801 ccggcaagag caacaaacgc aagtacagca acctgtcatt aaactgggaa atttaatctt
    34861 tgcccgttgc ataaccttta attattcata
//
LOCUS       DBANUU010000039        34464 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000039 DBANUU010000000
VERSION     DBANUU010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.039"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..668
                     /locus_tag="ACIE68_001814"
     CDS             <1..668
                     /locus_tag="ACIE68_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFX5908141.1"
                     /translation="FAPFLAQSNMTVYPMAVSINSQGEGNVRVISKSNEVQYIKATVF
                     RIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVRFVAMEPEQKEKNYRVKF
                     EAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKLEVNAAQKLVNAGNQRLK
                     ILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKIVVKYNNWITKDNGEFEL
                     AVH"
     gene            698..1198
                     /locus_tag="ACIE68_001815"
     CDS             698..1198
                     /locus_tag="ACIE68_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFX5908142.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1290..3974
                     /locus_tag="ACIE68_001816"
     CDS             1290..3974
                     /locus_tag="ACIE68_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFX5908143.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            3971..5059
                     /locus_tag="ACIE68_001817"
     CDS             3971..5059
                     /locus_tag="ACIE68_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFX5908144.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5147..6445)
                     /gene="exuT"
                     /locus_tag="ACIE68_001818"
     CDS             complement(5147..6445)
                     /gene="exuT"
                     /locus_tag="ACIE68_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFX5908145.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            6928..8340
                     /gene="uxaC"
                     /locus_tag="ACIE68_001819"
     CDS             6928..8340
                     /gene="uxaC"
                     /locus_tag="ACIE68_001819"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFX5908146.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8355..9842
                     /gene="uxaA"
                     /locus_tag="ACIE68_001820"
     CDS             8355..9842
                     /gene="uxaA"
                     /locus_tag="ACIE68_001820"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFX5908147.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9925..10476
                     /gene="ygjV"
                     /locus_tag="ACIE68_001821"
     CDS             9925..10476
                     /gene="ygjV"
                     /locus_tag="ACIE68_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFX5908148.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEVSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10481..11725)
                     /gene="sstT"
                     /locus_tag="ACIE68_001822"
     CDS             complement(10481..11725)
                     /gene="sstT"
                     /locus_tag="ACIE68_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFX5908149.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12124..13089)
                     /gene="alx"
                     /locus_tag="ACIE68_001823"
     CDS             complement(12124..13089)
                     /gene="alx"
                     /locus_tag="ACIE68_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFX5908150.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(13372..14358)
                     /gene="ygjR"
                     /locus_tag="ACIE68_001824"
     CDS             complement(13372..14358)
                     /gene="ygjR"
                     /locus_tag="ACIE68_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFX5908151.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14437..15120)
                     /gene="ygjQ"
                     /locus_tag="ACIE68_001825"
     CDS             complement(14437..15120)
                     /gene="ygjQ"
                     /locus_tag="ACIE68_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFX5908152.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(15197..15700)
                     /gene="ygjP"
                     /locus_tag="ACIE68_001826"
     CDS             complement(15197..15700)
                     /gene="ygjP"
                     /locus_tag="ACIE68_001826"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFX5908153.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15785..16921
                     /gene="rlmG"
                     /locus_tag="ACIE68_001827"
     CDS             15785..16921
                     /gene="rlmG"
                     /locus_tag="ACIE68_001827"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFX5908154.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            17112..17195
                     /locus_tag="ACIE68_001828"
                     /pseudo
     CDS             17112..17195
                     /locus_tag="ACIE68_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            17192..17608
                     /gene="higA"
                     /locus_tag="ACIE68_001829"
     CDS             17192..17608
                     /gene="higA"
                     /locus_tag="ACIE68_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFX5908155.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17653..19671)
                     /gene="fadH"
                     /locus_tag="ACIE68_001830"
     CDS             complement(17653..19671)
                     /gene="fadH"
                     /locus_tag="ACIE68_001830"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFX5908156.1"
                     /translation="MSYPSLFASLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSAFQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPSVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(19997..22348)
                     /gene="ygjK"
                     /locus_tag="ACIE68_001831"
     CDS             complement(19997..22348)
                     /gene="ygjK"
                     /locus_tag="ACIE68_001831"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFX5908157.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDHLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22365..23435)
                     /gene="ygjJ"
                     /locus_tag="ACIE68_001832"
     CDS             complement(22365..23435)
                     /gene="ygjJ"
                     /locus_tag="ACIE68_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFX5908158.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(23569..25002)
                     /gene="ygjI"
                     /locus_tag="ACIE68_001833"
     CDS             complement(23569..25002)
                     /gene="ygjI"
                     /locus_tag="ACIE68_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFX5908159.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(25065..25514)
                     /gene="ebgC"
                     /locus_tag="ACIE68_001834"
     CDS             complement(25065..25514)
                     /gene="ebgC"
                     /locus_tag="ACIE68_001834"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFX5908160.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(25511..28603)
                     /gene="ebgA"
                     /locus_tag="ACIE68_001835"
     CDS             complement(25511..28603)
                     /gene="ebgA"
                     /locus_tag="ACIE68_001835"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFX5908161.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLSASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGSD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(28787..29770)
                     /gene="ebgR"
                     /locus_tag="ACIE68_001836"
     CDS             complement(28787..29770)
                     /gene="ebgR"
                     /locus_tag="ACIE68_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFX5908162.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQLLAQEDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            29989..30321
                     /gene="ygjH"
                     /locus_tag="ACIE68_001837"
     CDS             29989..30321
                     /gene="ygjH"
                     /locus_tag="ACIE68_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFX5908163.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(30363..31742)
                     /gene="ygjG"
                     /locus_tag="ACIE68_001838"
     CDS             complement(30363..31742)
                     /gene="ygjG"
                     /locus_tag="ACIE68_001838"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFX5908164.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            32160..33680
                     /gene="aer"
                     /locus_tag="ACIE68_001839"
     CDS             32160..33680
                     /gene="aer"
                     /locus_tag="ACIE68_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFX5908165.1"
                     /translation="MSSHPYVTQQNTPLAGDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVMLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(33834..34457)
                     /gene="nfeR"
                     /locus_tag="ACIE68_001840"
                     /gene_synonym="yqjI"
     CDS             complement(33834..34457)
                     /gene="nfeR"
                     /locus_tag="ACIE68_001840"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFX5908166.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
BASE COUNT         8532 a         8765 c         9017 g         8150 t
ORIGIN      
        1 tgttcgctcc ttttttggca cagtcaaata tgactgttta tcctatggct gtttccatta
       61 atagtcaggg tgaaggcaac gttcgcgtaa tatctaaaag taatgaagtt cagtacatca
      121 aggcgacagt attccgtatc gataatccat cgacgcctca ggaaaatgaa gttgagatta
      181 aatccggtga tgcaaatcat ctggttgtta tgccacctaa attcgcttta ccagccggta
      241 gtagcaaaac cgtacgtttt gttgcgatgg aaccagagca aaaagagaaa aattatcgcg
      301 ttaaatttga agcggttccc agtattgatg acgttgccac agataaaaaa gatctctcta
      361 tgcagttaac agttaactta atttggggga ttgttgttag tgttccacct cagcaaccta
      421 ttgctaagtt agaagtaaat gctgcccaaa aattagttaa tgcagggaat caacgcttaa
      481 agattttaac gatcgcttat tgtaaaaata atagcaaaga aaattgtaag atacagaccg
      541 taaataaaaa catcttccct ggtcaggaaa gaaatcttga aagcatatct ggctacgaca
      601 aaatcgttgt caaatataat aactggatca ccaaagataa tggcgagttt gaactggcgg
      661 tccattaatt atcattgtaa taaacgaagg aatagtaatg aaaaaggtat ttgcaaaatc
      721 tcttctggtc gcagcgatgt tttctgttgc aggctccgca ttggcagtac aaaaggatat
      781 tactgtaacg gccaacgttg atgccgctct ggatatgaca cagaccgata acaccgcgtt
      841 gccgaaagca gtagaaatgc aatatctgcc ggggcagggt ctgcaatctt accagttgat
      901 gaccaaaatc tggtctaacg acgttaccaa agatgtaaaa atgcagttag tgtccccagc
      961 gcaactggtt caaagcctgg atgctagcaa gattgttccg ctgacagtaa cctggggcgg
     1021 tgaagaaatt aaagctgatg cggcaaccac ttttaccgca accaaaatct ttgcttccga
     1081 tgcgttaact aacggttctc tggctaaaaa cctgatgttt gctcaaacca ccaaaggtgt
     1141 tctggagaca ggcatctacc gtggtgtagt aagcatttat ctgtctcagg atatctaatc
     1201 cagataacag aattaacacg taacaaaggg aggggtagcc ctcccttatt ttaactgttt
     1261 tagtatttgt tatggatttc aggattgcca tggataaaaa attactggct cttttgatcc
     1321 tggcgagtct cagcccggca gaggcgacat taaccaaaat tcccgcaggg tttgaggtta
     1381 ttgctcaggg acagcaggag tatatcgagg tttatttttc agggaaaagt ctcggtaaat
     1441 attatgcaat ggttaatctt gataccgtaa catttcttga tccagcaagt ctatataaca
     1501 agctggagct ggatgtagac gatcagaaaa tcgcgcatat agtgaaagaa aaattatcgc
     1561 agccgctagc tcgccacggt gaattggctt gcggttatgt acgtactgac tcagggtgtg
     1621 gttttctgaa taccgatacg ctggaaataa tctataatga tgaagaaagt tcggcaacgt
     1681 tgtttattaa tccgcaatgg aattcagctt tcgatgcgaa gtcattatat ttaaatccag
     1741 acaaaaatac tgttaatgct tttatacatc agcaagacat caatgttctg gcacaggatg
     1801 attaccaatc gttgtctatt cagggaaacg gtgcgctggg aataacagaa aatagctata
     1861 ttggtgcaca ctggaatttc aacggttatg atgcagatga tgtcagtgac agtaatgctg
     1921 acgtcagcga tctctattat cgttatgatt ttttacgtcg ttattatgtg caggcgggtc
     1981 gcatggacaa ccgcacacta tttaatgcac aaggcgggaa ctttaccttt aactttctgc
     2041 cactcggtgc aattgatggg atgcgtatcg ggtcgacact gagctattta aaccaggcgc
     2101 aaagccagca aggaacccca gtaatggttc tgctttcgcg caattctcgt gttgacgctt
     2161 atcgtaatga gcaacttttg ggatcgtttt atctcaatag tggttcgcaa tttattgata
     2221 ccagttcctt tccgcctggc agctacagcg tggcattaaa agtctacgaa aataaccaac
     2281 tcacccgcac cgagcttgtg ccgtttacca aaaccggcgg tctgactgac ggaaatgcgc
     2341 aatggttctt acaggcaggt aaaactacat cacaggtttc tgatgatgaa agttcagctt
     2401 atcagctagg agtacgcctg ccattacatc cgcaatatga gctctacgca gggctggcga
     2461 atgccgatga tgtgagtgct ttcgagttag gtaataactg gacggcagat ttaggcgggg
     2521 tggggaatct tgcaatcagc gccagcgtgt tccgtaacga tgacggcggc aaaggtgata
     2581 tgcaacaggc caactggagt aatccgggat ggccgacgtt gggcttttat cggaccaact
     2641 ctgacggcga tgcttgtaca accgacagca gagagagcta taacgcctta agctgttatg
     2701 aaagtatttc cgcgacggtt tcacagaatt ttgtcggctg gaatatgatg ctgggttata
     2761 cccgcacaca aaataacact gatgatagtt tgcgttggga taaacagcag agctttgaaa
     2821 ataactatct tcgccagaca acagcgcaaa gtatctctga aactgtacaa cttagcgctt
     2881 cccgcgcttt tgtgatgcgt gactggattt tgagtacgtc agtcggtgtt ttccatcgta
     2941 atgacaacgg tggcgataac gacgacaacg gcttgtactt atcgttttcg ttatctgaca
     3001 cgccaacgat ggatagcaat aacaacagcc attcaacaaa tgtgtctacg gattatcgtt
     3061 atagcgaaca ggatggcgat caaacgtcat ggcagttatc gcataccttt tataacgatt
     3121 cattcagcca taaagaactt ggcgtaaccg tcggaggcct gaacaccgat accataaaca
     3181 gcgcggttaa cgggcgttgg gatgggcaat acggaaatgt ctacgctacc gtatctgata
     3241 gttatgaccg taagaatcat gatcatcttt cggcctttac gggcacttat agctctacgc
     3301 tggctgtcag tcgctatggc gttaatttgg gagccagtgg tacagacgat ttgctgggtg
     3361 cggtactggt ggatgtgaaa ggcttctctg aacaggatga agagagtcag gatctgcaac
     3421 tcgaagcgcg ggtggcaggc agccgaactt tgcagcttgg tcaaagtgac agtgtgttgt
     3481 tcccttatcc tggatttcag tctggttttg ttgaggttaa cgacagtagc cagggcaatc
     3541 agcaggggac aacaaacatc attaacggtg cagggaatcg tgaattaatg ttgttgcctg
     3601 gcaagctgcg ctatcgcgaa gtgtctgcca gctttaacta caactatatc ggtcgtttat
     3661 tactgccggc agcggtgaaa aaattcccga ttgtggggct gaacagcgcc atgttactgg
     3721 tagctgaaga tggcggattt acacttgaaa ttaacggcag cgaaaaagag ctctatctgc
     3781 tttccgggca gcaattcctt aagtgtccac taagtgttgt aaagaaacgc gccagcattc
     3841 gttacagcgg agatgttact tgtagtgtgg tgacttattc acaattaccg gagtcgattc
     3901 aggttcaggc acagttgaaa cagcctaaat tacgtggaaa cgttcagacg gcgcaaaggg
     3961 aggttgcacc atgagaaatc gattgattgc ggcgatattg ggcttgtttg gcacgctcac
     4021 tggcgttcag gcagctcctg acgtgaccag tgaaattacg tatgatttgg catctggtag
     4081 agcggattat tacttctgga aggatgaagc gtcggcagga aataatggat atatgtggta
     4141 tgaatgttcg tatcctgacc tccaacaaac ctgtacagct aatggaaata tatcgacagt
     4201 acaaatctat ttaactgaac aacgcagtgg gatgcgttgg ccggtaaaac tcaaaggatt
     4261 taaaacagcc attgtaagta gcgatgaagc gccgccagga tgcaaggggg gcaaagggct
     4321 tcagacgaat cttaaggatt ctaatagatc ttcatgtaca gaagatggtc aacattatta
     4381 tatatacgat acaaagtttc ttacgctcta ccttgagcag acagagatga agaatttgcc
     4441 gattggtggc gtctggaagg ggaaagttaa attacattcg aacagcccgg cccaggacta
     4501 tttcgcaaat attaccctga atacgctcga ccccaaccat attgacgtgt tcttcccgga
     4561 gttcgcccac gccacgccaa gggtgcagtt agacttgcat ccaacaggaa gcgttaacgg
     4621 cagcaactac gcgcaagatc tgaccatgtt ggacatgtgc ctgtacgatg gttttaacgg
     4681 taatgccatc agttatgaaa tcatgctcaa agatgaaggg cgaccagctg cagggcgcag
     4741 agacggttac ttctctatct atcgtcaggg agggaccacc accgacgagg gagaacgcat
     4801 tgattaccgg gtcaaaatgt acaacccgga aaccggtggg caaattgatg tgcgcaataa
     4861 tgaaaatatg gtctggaaca gcattaacct gaaacgtgtg cgtccggtgg tactgcccgg
     4921 tattcgctat gccgtgatgt gtgtgccgac acccttgact ctggcagtcg ataaattcag
     4981 cgtgatggat aaacaggccg ggtactacat gggcaaattg tcagtaatct ttacgccttc
     5041 cttgccaacc atcaattaac cctgcaaagc ggacaaacgc ttttttctat ataaacgacg
     5101 aagccgtctg ataaagcggc ttcgtcatta caacttaagt taaacgttaa tgttgcggtg
     5161 cgggatcgtt atgtgtttcc tgcgccacct caatcgccgg tttgttctgc aacacggtcc
     5221 agataaccag cgcacctaac aggtcaaata ctgccagaac tgcgaacagc gggctgaagc
     5281 cgatggtgtc agccagtgca ccgacaacca gcgcaaacag cgtacttgcc agccatgcgg
     5341 acatcccggt taaaccgttc gccgtcgcca cttcgttacg accgaacacg tcagaagaga
     5401 gcgtaatcag cgcgccagac agtgcctggt gggcaaaacc accgatacac agcagcataa
     5461 ttgcgacata cgggttggtg aacagaccga tcatacccgg gccaatcatc agcacagcac
     5521 ccagcgttac gaccatctta cgggaaacga tcaggttcac accaaaccaa cgctggaaca
     5581 gcggcggcag gtaaccaccg aggatacaac cgaggtcagc aaacagcatc ggcatccagg
     5641 cgaacatcgc gatctctttc aggttaaagc cgtaaacttt aaacatgaac agcgggatcc
     5701 acgcgttaaa agtaccccag gccggttctg ccaggaaacg cggcagcgcg ataccccaga
     5761 actgacggtt acgcaggatc tgaccaacgg acattttctt cgccgtgctc acctggtgct
     5821 gggcttcctg accattaata atatagtcgc gttcttcatc ggtcagatgc ttctggtcgc
     5881 gcggatgttt atagaaaatc agccatgcca tcgcccagat aaagctcaat gcaccggaga
     5941 tgataaatgc catctgccag ctgtgcatta cgattgccca taccaccagc ggcggcgcaa
     6001 tcatcgcacc aatcgaagaa cctacgttaa agtaacctac cgcgatggaa cgctctttcg
     6061 ccggaaacca ttcggagctg gctttcagac ccgccgggat catcgctgct tccgcggcac
     6121 cgactgcacc acgagcaatc gccaggccac cccagctacc agccagcgcg gttgcaccac
     6181 agaatacggc ccacagcaca gcaaacattg cataaccgat tttcgtaccc agcacatcca
     6241 gtacatagcc cgctaccggt tgcatgaccg tataagcagc agaataggct gcgatgatat
     6301 aggaatactg ttgggtggag atgtttaact cttccatcag agttggcgca gctgccgcca
     6361 cagtgttacg cgtcaggtaa ccaagcacgg tgccaagcgt caccagtgcg atcatatacc
     6421 aacgtaaccc tttaatttta cgcatgtaaa acctcatcgt tttgttatat ccgcaggaag
     6481 cgcggccatc atcccgccag aggctgaaga tgttagttac gaccgggtaa ccgaaaaaaa
     6541 accggcacgc cccgaacgtt gccatgaaag tttcgtgcta attcggcttc cgtaccggtt
     6601 aacccgattc atctaacaaa tgaatgaatc ggcaatttgc attccgtcga cttatgattt
     6661 gcgacggcag aaagataact tgtcatacaa ctttaaaagg tgagagccat cacaaatgtg
     6721 cgaatatttg tagggacatt acctgacgac agcgaggcca gtactggcgc ggcctgcagc
     6781 gagatttacc actttgagag taattttttt aactacgttt attgatctaa ctcacgaaaa
     6841 tatcttcgga ctctggaaat tggtgtgata actttgtcag catcgcacca taagcaagct
     6901 agctcactcg ttgagaggaa gacgaaaatg actccgttta tgactgaaga tttcctgtta
     6961 gataccgaat ttgcccgccg tctgtatcac gactacgcaa aagaccagcc gattttcgat
     7021 taccattgcc atttgccgcc gcagcagatt gcggaagact atcgttttaa aaacctgtat
     7081 gacatctggc tgaaaggcga tcactacaaa tggcgcgcta tgcgtaccaa cggtgtggct
     7141 gagcgtctgt gtactggtga tgcgtctgac cgtgaaaaat ttgacgcctg ggcggcgact
     7201 gttccgcaca ctatcggcaa cccgttatac cactggacgc acctcgaact gcgtcgtccg
     7261 ttcggtatca ctggcaaatt gctttctccg tcaactgccg atgaaatctg gaacgaatgt
     7321 aacgaactgc tggcgcagga taacttctcc gcgcgcggca tcatgcagca gatgaacgtg
     7381 aaaatggtcg gcaccaccga tgacccgatc gattctctgg agcatcacgc agagatcgcc
     7441 aaagacggct ctttcaccat caaagtgctg ccgagctggc gtccggacaa agcctttaac
     7501 attgaacagg cgacctttaa cgactacatg gcgaagctgg gcgaagtttc cgataccgac
     7561 attcgccgct ttgctgacct gcaaactgcc ttgaccaaac gtctggatca cttcgccgct
     7621 cacggctgta aagtgtctga ccatgcgctg gacgtggtga tgtttgctga agcgaacgaa
     7681 gcggaactgg acagcattct ggcgcgccgt ctggctggcg aacccctgag cgagcacgaa
     7741 gtggcacagt tcaaaactgc ggtactggtg ttccttggtg ccgaatatgc acgtcgcggc
     7801 tgggtacagc agtaccacat tggcgcgctg cgtaataaca acctgcgtca gttcaaactg
     7861 ctggggccgg atgtaggctt tgactccatc aacgaccgtc cgatggcgga agagttgtct
     7921 aagctgctga gcaagcagaa cgaagaaaac ctgctgccga aaaccattct ctactgcctg
     7981 aacccgcgcg ataacgaagt gctgggcacc atgatcggta acttccaggg cgaaggtatg
     8041 ccgggcaaaa tgcagttcgg ttccggctgg tggtttaacg atcagaaaga cggtatggaa
     8101 cgtcagatga cccaactggc gcagctcggt ttgctgagcc gctttgtcgg tatgctgact
     8161 gacagccgta gcttcctgtc atacacccgt cacgaatact tccgccgcat tctgtgccag
     8221 atgatcggtc gctgggtgga agcgggcgaa gcaccggcgg acatcaacct gctgggcgag
     8281 atggtgaaaa atatttgctt taacaatgcg cgtgactact tcgccattga actgaactaa
     8341 ggtctggggt tgatatgcaa tacatcaaga tccatgcgct ggataacgtc gcggtcgctt
     8401 tagcagattt ggctgaaggc acagaagtca gtgtcgataa ccagactgtt acgctgcgcc
     8461 aggatgttgc tcgtggacat aaatttgcgt taacggatat cgcaaaaggg gccaatgtca
     8521 tcaaatatgg cctgccgatt ggttatgcat tggcggatat tgcggcgggg gaacacgtcc
     8581 acgcccacaa tacgcgcacg aatctgagcg atctggatca gtatcgctat caacctgatt
     8641 ttcaggatct gcctgcgcaa gcggcagatc gtgaagtgca gatctatcgt cgcgctaacg
     8701 gcgatgtcgg ggtgcgtaac gagctgtgga tcctgccaac cgtgggctgt gtcaacggca
     8761 tcgcgcggca aatccagaac cgtttcctga aagagaccaa caacgccgaa ggtaccgacg
     8821 gcgtgttcct cttcagccac acctacggct gctcacaact gggcgacgat cacatcaaca
     8881 cccgcaccat gctgcaaaac atggtgcgcc acccgaacgc gggcgcagtg ctggtgattg
     8941 gtctgggctg tgaaaacaac caggttgccg cattccgtga aacgttgggc gatatcgatc
     9001 ctgaacgcgt tcatttcatg atctgccaac agcaggatga tgagatcgaa gccggaatcg
     9061 agcatttgca tcagctgtat aacgtgatgc gcaacgataa acgcgagcca ggcaaactca
     9121 gcgaactgaa gtttggtctg gagtgcggtg gttctgacgg tctttctggt attactgcta
     9181 acccgatgct ggggcgtttc tctgactacg tgattgctaa cggcggtact accgtactga
     9241 ccgaagtgcc ggagatgttt ggcgcagagc agttgctgat ggaccattgc cgcgacgaag
     9301 caacgtttga aaaactggtc accatggtca acgacttcaa acagtacttt attgcccatg
     9361 accagccgat ctatgaaaac ccatcgccgg ggaacaaagc gggcggtatc accacgctgg
     9421 aagataaatc ccttggctgt acccagaaag cgggttccag cgtcgtggtt gacgtgctgc
     9481 gttacggcga gcgtctgaaa acgccggggc tgaacttgtt aagtgcgccg ggtaacgatg
     9541 ccgtagcgac cagcgccctg gcgggtgcgg gctgccatat ggtgctgttc agtactggtc
     9601 gtggcacgcc atacggcggc tttgtaccga cggtgaaaat cgctaccaac agtgaactgg
     9661 cggcgaagaa aaaacactgg atcgactttg acgcgggtca gctgatccac ggtaaagcga
     9721 tgccgcagtt gctggaagaa tttatcgaca ccatcgttga gtttgccaac ggtaaacaaa
     9781 cctgcaacga gcgtaacgac ttccgcgaac tggcgatctt caaaagcggc gtaacgctat
     9841 aaagtcgaaa tgagttgtta ataaaaaacg gcgtttcata atgtgaagcg ccgtttgctt
     9901 ttccatacct gctaaggatt tatcatgacc gcgtattggc tggcccaggg cgtgggtgtc
     9961 atcgcctttc tgattggtat cacaacattt ttcaatcgtg acgaacgtcg cttcaaaaag
    10021 cagctttcgg tctatagcgc cgttattggc gtacattttt ttcttctggg cacctatccc
    10081 gctggtgcca gtgccatcct taatgccatt cgtacattga ttaccttacg cacgcgcagc
    10141 ttatgggtaa tggcgatttt tattgtgctg actggcggaa ttggcctcgc gaagttccat
    10201 catcctgtcg aactattgcc ggttatcggt acgattgtca gtacctgggc gctgttccgc
    10261 tgtaaagggc tcaccatgcg ctgcgtgatg tggttttcaa cgtgttgctg ggtgattcac
    10321 aacttctggg cggggtcgat aggcggcaca atgattgagg ttagttttct gcttatgaat
    10381 ggcctgaata tcattcgttt ctggcggatg cagaaaaggg gaattgatcc gtttaaagta
    10441 gagaaaaccc ctcccgccgt agacgaaagg ggttaaacaa ttaattacgc agggcgctat
    10501 ttgccagacg atcgtcttcc gcctggcaag ctgccgcagt gaacagcacg tcagttgaag
    10561 agttcagcgc ggtttcgcaa gagtcctgca atacgccgat gataaagccg acggcaacca
    10621 cctgcatggc gatatcgttc gaaataccga acatattaca ggccagcggg atcagcagca
    10681 gagaaccccc cgccacgccg gatgcgccac aggcacacag agaagccacc acgctcaaca
    10741 gcagcgccgt gggcagatcg accgggatac ccagcgtatt aaccgcagcc agcgtcaaca
    10801 cggtaatggt gattgctgcg cccgccatat tgatggtggc acccagcgga atagaaacgg
    10861 aataggtatc gcgatccaga ttcagctttt cacacagtgc catattcacc ggaatgttcg
    10921 ccgcagagct gcgggtgaag aaggcataca cgccgctttc gcgcaggcac agcagcacca
    10981 gcgggaacgg gttacgacga attttccacc acaccagcaa tgggttaacc accagcgcca
    11041 ccagtaacat acagccaacc agcacgacca gcagttgcgc gtagccccac agtgtggaga
    11101 aaccggtggt tgccagggta gaagaaacca gcccaaaaat accaatcggt gcgaagcgaa
    11161 tgaccagttt caccataaag gtaacggcat tcgacatatc gttaaccagg tttttggtgg
    11221 tctcgttacc gtgacgcagt gcgaagccga ggccgatcgc ccacaccaga atcccgatgt
    11281 agttaccttt cagcagcgcg tcgatggggt tggaaaccat gctcattacc agcccgcgca
    11341 tcacttcgac aatgcctgac ggcggcgaaa tatcacccgc gctactggat aaatgcaggg
    11401 tagaagggaa ggcaaagctg aagactactg cggccagagc agcagagaag gtgcccagta
    11461 gatagaggaa caaaataggg cggatattgg ttttctgccc gtgctggtgg ttagcaatag
    11521 atgccattac cagcatcaac accaggatgg gggcaacggc tttcagtgcg ccgacgaaca
    11581 aagtacctaa cagaccaaca gcttccgccg cgggttttga gatccatgcc agaagaatcc
    11641 ccagaacaag gccgaccagg atttgtttta ccaggctgcc atgagccaga cgccggaata
    11701 gtcccggtga acgttgcgta gtcatttttc gatcctttca ttgtgttgtc gcacatccct
    11761 ggtgcgttct gtcgacgcat ctttcaggat gtaacaaagt gtttgcaagg tcgagtataa
    11821 ggaaagttac agcttcggga agcgaaaaat gctggatttt aaagagaggt catattttgc
    11881 aacttaatgg ttacatttat ttgacataaa aataaagcca acgtgcggct tccagactga
    11941 tgacaacatc aaaaaaggac tcggtcagtc ccctcgcccc gtttggggag agggttaggg
    12001 tgaggggaac aggccagcat cggtgcgaaa aattaccctc accccgaccc tctccctgga
    12061 agggagaggg agaaaacggt gctcatgtta atgacagggt acacggctta ggcagattaa
    12121 aaattatcca ccccgctgct tatcatgccg ataattcacc caggcgttga taataaacgt
    12181 catcaccaga atgccaaaca ccacgcccag cgagacggcg attggaatat ggtagaagtc
    12241 gacaatcagc atcttgatac cgataaacac caggatcacc gccaggccat atttgagcat
    12301 cgagaaacgc tctgctacgc ccgccagcag gaaatacatc gcacgcaggc cgaggatcgc
    12361 aaacaggttt gaggtcagca caatgaacgg gtcagtggtc acggcgaaga tagccggaat
    12421 gctatccacg gcgaaaatca cgtcgctcaa ttccaccaga atcagtacca gcatcagcgg
    12481 tgtggcgtac agcaatccat tcttacgcac aaagaaatgc tcgttgtcga tggtgtcggt
    12541 catgcgcaaa tgaccgcgta gccagcgcac cagcggcttg tcgccaatgc ctgattcatc
    12601 ttcatgggcg agcgccatct tcacgccggt aaacagcagg aaggcaccga agatatacag
    12661 gatccagtcg aactgcgaaa ttagccagct gccagtgaag atcatgatgg tacgcagaac
    12721 aatcgccccg agcacgccat acaccagcac gcggcgttgt aatgccgccg gaacagagaa
    12781 atagctgaac aacatcagcc agacaaagac gttatcgacc gccagcgatt tctcaatcag
    12841 ataacctgtg agaaaggcca gtgcctgtgg atcggcaacg gcgcgacctt cggtttgcac
    12901 cagataccac cagaaagcgg cgttaaacag taacgacagc gtcacccaga ccagcgacca
    12961 ggccgcagcc tgtttcatgg tcatggcatg tgccccacga cgcccctgca acaacaggtc
    13021 gatagccagc ataatggcga caacaacagc gaatccgccc catagcaacg gcgtgccgac
    13081 agtattcata gaagttcctt acacataaaa aaacggccaa cgtcggaaga cgtcagccgc
    13141 tgctttttat gcatagacct cgccttccgg caaggtctca cttacaacaa aaaaggaata
    13201 cgtctgcgta ttcctttcgg gttgcccggt gaccggatgt ggtttttcac acatcgtaat
    13261 gacgatcgac cggcaatgaa gttactcccc tttgcaggta acaaagtatg acagaccatt
    13321 cagccagtca atggtctgtt acataccttt tacactgttt tactttggca attatagttt
    13381 tacgctatct gccggaaaaa tcaccccagt ctggcggcgg atctcggtca gcagtttggc
    13441 ggtgatgcga ctgaccgcca gccccggatg atccaccaga tgctcatcca ccagggtagc
    13501 gaacagctct gcttcgtaga gcatggtatt aatatgctgc ggctgggtga gatcctgcat
    13561 ttggctgcca cgcggcacga agcacacttt ctggcattca gacagttttt caatcaccag
    13621 cgatcctgct tcgccctgaa tctcgctcgc caggacagaa tcactgactt ttgagtgctg
    13681 caaggtgacg ctgaaatcac cgtaatccat caccaccacg ccgtgggcat ctacaccgct
    13741 tgccagcaaa ctggcggttg cctgcacgct tttcggctca ccaaataacg ccaccgccga
    13801 cgccaggcag taaaagccga tatccataat tgaaccgtta gagaatgacg gattaaaggt
    13861 gttgggattt tcaccgtcca ggtaacgttg atagcgcgag gaatactggc aatagttgaa
    13921 aaagacttta cgcaatttgc cgactttcgg cagcgcctgg cgcagcaaat gaaagttcgg
    13981 caggcaggcg gttttaaatg cctcaaacag caccacctga ttttcccgcg cacaggcaat
    14041 ggcggcatcc acttccgcca gattcgacgc cagtggtttc tcgcaaatca catgaatttt
    14101 atggctaagg aaaagttgtg tctgggaaaa gtgcagggca ttcgggctgg caatatacac
    14161 cgcgtcaatg gcatcgcttt ccgccatcgc ttccagcgag gtaaacagat gctcgacaga
    14221 aaaatcattg gcgaagtgct gagcctgctc aaggctgcgg gaatatacgg cggttaactt
    14281 gtatttaccg ctctcatggg cggcctcgac gaactggcga gtgatccagt tcgtaccaat
    14341 cacagcgaaa cgtatcataa cgcgtggata ctccataaag ggattctgtc gtcaatgtag
    14401 cacgcctttt tcatcttcga cgtgcgcaga tacgcattat tcttttgccg gacagccatg
    14461 ctcactaaac ggcccaatca tgaccgatgg cccgagatat ttcggctggc gatggaggat
    14521 tttggcgtca atcacggcgc tgacacgagc gagtttctcc cgcagttgag tataaaaacc
    14581 gtgcttcata ttgaggtcag gcgcgttaaa accgatagcg tcgatgccgt actgttttgc
    14641 cagccagatg gcgcgttgat tatgaaattc ttgcgagata atggtgatat ggttttcacc
    14701 aaaaaccttt ttggcacgca ctaccgaatc cagcgttgag aatccggcat agtcgcagaa
    14761 gatcactttc gccggtacgc ctttagcgat caaagcctgc tgcatacctg aggcttcatc
    14821 gtaattttta cgtccgttat cgccacttac cagtagccat ttcacttttc cggcgtggta
    14881 caacgctgct gcggtatcaa tacgtcgggt aaagtagcga ttacccggcc tggcccccag
    14941 caataagccc acgttacgcg ccggaactgc gttgacatcg ctccaggtca gctgcttgct
    15001 ggcgttgacc ataacgcgat cagcaataaa aatggtcgct cctgctataa gcaacaacaa
    15061 acagccaatc ttaagagtcc ggcgagaaaa acaaatacgg agaagaaggc ggggctgcat
    15121 ggtcgttatc cttaacgaaa ggggggaggt cgattttagg cgctaaagaa agaaaagata
    15181 acgcctgaca tcattttcag attttgtcct gcccaagcga caactgcgtt agccacagcc
    15241 gggtgtcgaa ctcaagctgg tggtactgcg gctccatatg gcagcacaac tggtaaaacg
    15301 ctttgttatg ctctttctcc ttcaggtgcg ccagttcgtg caccacgatc atacgtaaaa
    15361 acggttccgg cgcgttgcga aacacagtag cgacgcggat ctctgctttt gctttcagct
    15421 tgccgccctg cacacgagaa acagcggtat gtaacccgag cgcgtttttt agcacatgga
    15481 tcttattgtc atacatcact ttattgatcg gcggggcatt gcgcagaaac tgatttttta
    15541 gatcctgagt atattgccag agggctttat cggtggcgta gtcgtgtgtt cccggatagc
    15601 gttttgccag cacatcgccc agacgctgtt cgttaatcag cgttcgcacc tgggaaagta
    15661 gctgctcggg ataaccctga agataagtaa gattgctcat aaccgcccac gaaaaagaga
    15721 aaaagggtat actcacgcac ccttttcagg ggcaacgtcg aaattttatc attcaggagg
    15781 gccgatgagc cacttagaca acggtttccg ttcactgaca ctacaacgtt ttccggcgac
    15841 ggatgacgtt aacccgctac aggcgtggga agcggcggat gaatatttgc tgcaacagtt
    15901 ggacgacaca gaaatccgcg gcccggtgtt gatcctgaat gatgcctttg gtgcgttaag
    15961 ctgcgcgctg gcggaacata agccgtacag cattggcgac tcatacatca gtgaactggc
    16021 gacgcgcgag aatttacgcc tcaacgggat tgatgagtcg agcgtgaagt ttctcgacag
    16081 caccgccgac tacccgcaac agccgggtgt ggtgctgatc aaagtgccga aaacactggc
    16141 attgctggaa cagcaactgc gtgcgctgcg caaagtggtc acgtcggata cacgtattat
    16201 tgccggtgcc aaagcccgtg acattcacac ttccacgctg gagctgttcg agaaagtgct
    16261 cggcccgacc accactacgc tggcatggaa aaaagcgcgc ctgattaatt gcactttcaa
    16321 tgaaccgccg ctggccgatg cgccgcagac cgttagctgg aagctggaag gtactgactg
    16381 gactatccac aaccatgcga atgtcttctc ccgcaccggg ctggatattg gcgcacgctt
    16441 ctttatgcaa catctgccag agaatctcga aggtgagatt gtcgatctcg gctgtggtaa
    16501 cggcgttatt ggcctgacgc tgcttgataa aaacccgcag gcgaaagtgg tgtttgtcga
    16561 tgaatcgccg atggcggtcg cttccagccg aatgaatgtt gaaaccaaca tgccagaggc
    16621 gttggatcgc tgcgagttta tgatcaacaa cgcgctctcc ggagtggagc ctttccgctt
    16681 taatgctgtg ctctgcaacc cgccgtttca tcagcaacat gcgctgaccg ataacgtcgc
    16741 ctgggagatg ttccaccacg cccgccgctg cctgaaaatc aacggcgaac tgtatatcgt
    16801 tgccaaccgt cacctggatt acttccataa actgaagaag attttcggca actgcaccac
    16861 catcgccacg aataataaat ttgtggtgct gaaaacagtg aagctggggc gtcgtcggta
    16921 agattgggag ttacctgccg gatgcggcgt taacgcctta tccggcctac gttcagcacg
    16981 ttactcccag gccggataag acgcggcaag cgtcgcatcc ggcaatgtct gcccagttct
    17041 ggaatcgcgc ttccataaaa caaaaaatcc ctgtcaccat cctctacatt ctcttgttta
    17101 gcgttgttca acgtttatgc tacatacgtg aagttatgac gcataaagaa tacgatttct
    17161 ttaccgctgt tcatcgtact aaggggaaaa aatgattgcg attgccgaca tcttgcaagc
    17221 aggagaaaag ctaactgcgg tggcaccttt tctggcgggt attcagaacg aggaacaata
    17281 cacccaggcg ctggaactgg tagatcatct gctgctcaac gatcctgaaa accccttgct
    17341 ggatctggtg tgtgccaaaa taaccgcgtg ggaagaatca gcgcccgaat ttgcggaatt
    17401 taatgccatg gctcaagcca tgcctggcgg tatagccgtg attcgtaccc ttatggatca
    17461 atatggttta accctttccg atctgccgga aattggcagt aaatctatgg tgtcacgcgt
    17521 tttgagcggg aagaggaaat taacgctgga acacgctaaa aaattggcaa cgcgattcgg
    17581 catttctccc gccttgttta ttgattaacg cgacgtgccg gatgcgttgc atccggcgct
    17641 tcaaataacg atttaaatct ccagcgccag ccgtgttccc tgggcaattg cccgtcgtgc
    17701 gtccagctcc atagccacat cgcagccgcc aattaaatgc acagttttcc cgctatcaat
    17761 cagcggttgc gccagcgcgc ggtttggctc ttgccctgcg cagatcacca cattgtccac
    17821 tgctaatacc tgcgtttcgc cgttgatcac cacatgcagc ccgtcatcgt caatcttctg
    17881 ataacttacg cttgggatca ttttcacacc ccgcgagagc agggtggtgc gatggatcca
    17941 gccggtggtt ttgcctaacc cctgtcccgg tttgctggct ttgcgctgga gcatcacaat
    18001 ttgccgtggg ctacgcggga tctgcattcc ctgcgggctt aagccaccag cctgttgtag
    18061 gctgctgtcg atcccccact cattacagaa cctggcgata ttctggctgg tggattcgcc
    18121 cggctgactt aaatacatcg ccgtatcaaa accaatcccg ccacaaccga tgatggcaac
    18181 cttgttgcct accggcgctt tgtcgcgcag tacatcgaga taactcaata ccttcggatg
    18241 atcgatcccg tcgataggcg gaacgcgcgg cacgatccca ctggcgagga tcgtttcatc
    18301 gaacgcctgt aactgatccg ccgtcacggt gtgattgagt ttcagcgtca cgcccgtcac
    18361 ttcgatcatc cggtggtaat agcgcagcgt ttcgtaaaac tcctctttgc cggggatctg
    18421 tttggcgata ttaaactgcc cgccgatctc gctgtgggca tcaaacaatg ttacctgatg
    18481 cccacgcgcc gccgcgttaa tggcaaacgc cagcccagca ggtcccgcac cgaccaccgc
    18541 cagatttttt ttctgcacgg caggaaggat tggcattttg gtttcgtggc aggcgcgagg
    18601 attcaccagg cacgaggtga ctttgccaac gaagatctga tcgaggcagg cctgattgca
    18661 gccaatgcag gtgttgatct catcggctcg ccccgattgc gcttttgaca gcagttccgc
    18721 atcagcaaga aacggtcgtg ccatcgacac catatcggca tcgccgcgcg agagaatgtc
    18781 gtcggcaacc tgcggatcgt taatccggtt ggtggtcacc agcggcagcg agacgtggcc
    18841 tttcagtttg cgcgtgaccc aagtaaatgc gccgcgcggc acgggcgtgg caatggtcgg
    18901 aatacgtgct tcatgccagc caatgccggt gttgataatg gtcgcgcccg ccgcttcaat
    18961 ggcctgcgcc agttctaccg tttcggcaaa agtgccgccg ccttcgacca ggtcgagcat
    19021 cgacaaccgg tagataataa tgaagtcgtt gccgacgcgt tcgcgcaccg cacgcactac
    19081 ttctacggca aatcgcatcc ggttgcggta atcgccgccc cactggtcac tacgctgatt
    19141 ggtgcgcagc gtcagaaatt cgttgatcaa atacccttcg gaacccatca cctctacacc
    19201 gtcgtatcct gcctcccgcg ccagttgcgc gcagcgggcg aaatcgtcga tcagttgcag
    19261 gatctcttca tgggtgagtt cgtgaggaac gaaacggttg atgggggcct gaaatgcgga
    19321 gggagcgacc agatgcggtt ggtagctgta gcgcccggta tgcaaaattt gtaaggcaat
    19381 tttgccgcct tcctgatgta cggcttcggt aatggtgcga tggtgtggga tctggctggc
    19441 gtcattgagc attgcgccac cttccatgcc gacgcctgtt aaatctggtg caatgccgcc
    19501 gctaacaatc agcgccacgc cgtgacgggc gcgttcggca taaaacgcag ccagccgttc
    19561 agcaccgtcc gggtattcct ccagcccggt gtgcattgaa cccatcaaca cgcggttttt
    19621 taacgtggta aaacctaaat ccagcgaggc gaacagcgac gggtagctca taaaattgtc
    19681 cagtatgtaa aataattgtt atgtggtcgg atgagttcta atttagccgt cgccggagaa
    19741 aagggaaaag gggagtgcgg ggtttgtgat gggattcaaa aagaatacgc ccggtggtaa
    19801 cgccggatgc gacgcttgcc gtgttttatc cggcctaggg gattgcacat gtagggcgga
    19861 taaggcgttc acgccgcatc cgccagtggc gcggtgcagt tgccggatgc aacgcttgcc
    19921 gcgttttatc cggccaggag gattgcacat gtagggcgga taaggcgttt acgccgcatc
    19981 cgccagcagt tgcaaattat tgcttacgga aaaaatcgtt atacaacata tacaaatgcg
    20041 cggcactcca ggagaaattt ggtgcgcctt gctgtgcgcc tgtcagcggg ttgtaatttt
    20101 cctgaattgg tccatcggcg gttaaccctt tggcgtgccg gaagaacgta tccgccagct
    20161 tcagggcatc atcacgataa ccgtaacgct ccatcccttt cagaccaaac cagaactgat
    20221 ccacccatac gcgcccacgc cagtagatat cagcgccaaa agccggattg gttaacgccg
    20281 ccgttcccag cgggacaaag gtgttgaact ctttaggatc gagcatcacc ttcaccaccg
    20341 cgtcggcgtt ggcctgcgtt gccgcaccgt taaacagcgg cgaccagcct tccggccctt
    20401 taccgcgctc aacaatcggt ttgcccgcgc agccgttcgc cagcggttta tcttcaatac
    20461 gcacgtcata gtagaactgg gtagtcgggt cgaacataca ggtgttgatg tagtccgcga
    20521 gctgctgtgc caactggcga tagcgtttgg cctcttctgg cttaccgagg atggttgcca
    20581 tctccgccag ataatggtta tcgctgtaca tatagctggc ctgatccacc gactcctgca
    20641 atagcgagta gcccagcaac gttccgtcct gactgcggtt ttcggcgaat ttcaccgtcc
    20701 agtcgctacg tttgccgcca ttagcgacat atttatccaa ctgttctttg tcgataaacc
    20761 caaagacggc ggcatcatca cgccccgatt cccacgatgc agcaacctgt gccggaattt
    20821 ccagactgtc gtactggcct ttctccacca cgcgggcgta gttgttcagc ccagactgcg
    20881 tctcttcttt atcgcctttt ttcaccgtaa acagcatctc gccgctctca gtgttgtggg
    20941 ctttgtcgcg ggtcgcgcca tattccggca cgccgttgcc gttgtgatcg cggttacgta
    21001 accaccagtc gtgataggcc accagtttcg ggtacatctc tgccacccag gttttatctt
    21061 gggtgacgtt atacacttcc atcaccgacc aggcggcaag gctgggtttg gtattacgtt
    21121 cgttccagtt gccgccatcg ccaccacgct cggggctaag gttccacgct atcaggtcgg
    21181 ggacaaagcc cacatcctgc ggacgcacgc ggtcgcctgg ctggatctgc caggagaaga
    21241 ccgcgcggat attctctttg gcgatgtccg gattgaaatg cgccatcgca aacgcctgct
    21301 tccaggtatc ccacggccag gtctgattgc cggagaacca gcgcccggtc accgacggtg
    21361 tgacggtgtt aaatttcacc gccccgccag gcgaacgcca gttaccgttg agcgtttcga
    21421 tcgctttcac cgcgacgcgc gtctgttccg gcgtcgcatc cggattggtt aaccctttct
    21481 tcagatactc ttcccagcgt tgctgcgagg cggtgagata aaacgccgga cgcgccagaa
    21541 tatcgcggat ctgcatttgc tctttgctaa cttcctgaga ggtcagcaga tgggaatagg
    21601 tggtatagag cgtcgtcgaa ccgttgatat gtgccttact ggtaaagcga ttgccgttga
    21661 tttcagtctg caccggcagg gatttatgca cctgatattc tgattcaccg gaggtcagca
    21721 gatcccaggt ggcgcgcact ttgccaaagg tgactttcag gccatcacgg gtggcgctga
    21781 ttttgcgctg gtagtcaggg tattcgccag caatggtttt atcggaaagc ggtttccctt
    21841 ctttcgcttc cagtttttcc agcagttcgc catcccacac cagatccagc ggtttattgc
    21901 tgatgatttt agtttccagt agtgacgtgc gcggcgtggc gaagcgcaga gtcatttcga
    21961 cctgcacatc ttttgctgtc agtttttgca ccagcgcgcc gggaatactg tatgcctcca
    22021 gcgtgaagtc gactttcttg ccgtcctgcc agacggtcag gtggtcgaaa ttgctggcca
    22081 taaagttgat gtactcttcc gtcagcagcg caacgcctgg aaaaccgccc atggtgttag
    22141 ggccgtctgg caacagatga ccatgccagg ctccgagatc gaaaaacgga ttaaaacgct
    22201 gatgatcgtc gtaatcgtaa tccttcatgt actgcggcgc accagtacgg ttaatcacgt
    22261 ttttataatt gtcggcgtta gcggcatggg cggaaaaact tatcagcaga gcgcaggtta
    22321 ctggcgttaa aatagttttg attttcatga aattacagtc cttattacca gtagaaatat
    22381 tgcatcagga agatttggtc gctgggatcg tgagaggcat ttttcatgta gaagcggcta
    22441 tcaaaagccg ttgcgaaacg tccgccgccg tattcgtacc agataccaaa ctgcatgaaa
    22501 gaggtggttt catcgtcagc gtggtcaggg cgatgtttgg cgtaggtgta agcggtggag
    22561 agataaacgc cctttaatgc ttcatacatg gcgctcacca tcatcccact ttcacttccc
    22621 ggtacgtcca gcccgtcggc acgtccggta tggtgccagg cgcgagcggc aaagttaggg
    22681 gtaagcaggc cggtgagata gagttgtcct gtgccgtcgg taatttccag accattcatc
    22741 caggtgatgc cttcggcaag gtcatactgg atgtaaccgt tcatcattgc cggataggtg
    22801 tatttgtcgt cgtagcgatc gtacttgcca aaatgtagcc aggctttgct ttcgtcatga
    22861 tgtccggcag gtgtggcggt gacgctataa cgaaagttgc cgcttaagtt ttgcaccttc
    22921 agcgcagtca taatgtcgcg ggtattggga atgacgtagc ccagatcggg cgtgaaatcg
    22981 ccccaccatt gcaggtcatc cagcgaggaa tcctgacgca gactgagcat gatttcggtg
    23041 ccatcattgg tacgataacc gccatagaaa cggttaattc cgccctcgaa accaccccag
    23101 ctatggtcag gcacccaggc attgccgtcg tggtcggcct gcacggtcca gccttcgccg
    23161 taaatcaggc cgaaccagga gccgtacttc atctccaggc caccttcaat ataggtgccg
    23221 tcactgtatt tatgtttatt ctcaccctct aaatccatat atccaccaac ccctaattcg
    23281 ccgtaaaaac gaaatgaagg agaagatgtc gaggtaatgg cgggtgtttt tgtgtcgtca
    23341 tgttctgccg gacgtgcttc ttctcctgcc gcagaaaagg cgtagcaaaa cggcagagca
    23401 agtaatgctc tgcatggtgc agtgattaat ttcattgtgt taatccatga gctaaagaaa
    23461 aagtacagcc ttttatcgtt gtaattataa aaatataaag gcagaaacta ttttccctct
    23521 cctcatccag gaagagggaa aatgcctgct cattttttat tattctgctt aatcaacatt
    23581 gctggctggc gtcgcttcaa tatggcgctc ttcagccgtt aatcccttta tatatttact
    23641 gtatttccac cacgcaaagc caaggaagat aacaataccg ccgacgttat aaaaaatgat
    23701 ggtcagaata ttcgcgccag tcgggaatgt cgaagcgaca aaccctacgg caaatatcgc
    23761 aatcagcatt gaaacaacga taataccggt gcggcgggag cccatacgga aatcgcgtgg
    23821 caggtgatct aatttggcgc gtaaattcag gtaagccagc atgatgaata acggcggaag
    23881 catggacgct gcggcagtca tattaataat agtattcatc agatcctgca cggtattgga
    23941 accgagcatc gggataatca tcagcgggat gacgatcagg aactggatcc acgctgcgcg
    24001 cgccggaacg ccgttttcgt tcagttcgac ggttttctta ccaaagatgc cttccgggat
    24061 ttcggagaag aaaattttca ccggcgttgc ggtccacatc agcagggaac cgaacatcgc
    24121 ggtaaaggac accagaccga caaagcgatt catcaacgct tccggtagac caaaatacgc
    24181 cgccatgccg tggaatacct gcaccgatcc gccggtaaat ttcaactctt tgctgctgac
    24241 gaagacgtta atcagcaccg aggagacgga atacagtacg ccgataaaaa tcccggcgag
    24301 gatgatcact ttaacgaacg atttcgaacc gcctttgacg tcgttaacgt acacagcgac
    24361 ggactccgcg ccacctgcgg ccataaagat ccaggtggta acgccgagga acgcccagtt
    24421 gaagttcggg atcatcgcgt caacggtgat ggggtcagca ggctgtacgc cgccaaccag
    24481 cgccgtacct gccagtaaaa tgtaggagag cgttaacagc agcatcagcg ttgaagtgac
    24541 ggaggtaatt ggccccaaca ttttcgcccc gttggtggaa acccaggtgg agaaggcgaa
    24601 cagcaccata ctgataatgg tggtggcaac cggcgtcata atatattcgt agccgaggaa
    24661 ggcatacgaa gcataagcaa taacgcgcgg caacagtgag gtgaaaaaga acaggtttac
    24721 gaaccagtag gtataggcag taataaatgc ccaacgtccg cccagcgaac tttttaccca
    24781 cgcgtagaca ccggcttctg agtttttatt taacgaaaca aattctgcga tgatcagaca
    24841 gaagggaata aaataaaaaa tcgtcgcgag gaaaaacatc ggtgccgagg ccagtccaag
    24901 ctcaatatta ttattgataa cgttgttaaa gctgtaaacg gcggcaaaag tcagcgagag
    24961 caagccgaat ttgccgattg tattacgttt ggtatcagac ataacacatc tccgaaaatg
    25021 tgtagggtag cggtggcggc aaactccttt tgccgccgta gttgttattt gttatggaaa
    25081 taaccatctt cgatggtgac tttgagaacc acttttttga ccgcgttatt gcagataaac
    25141 cgatacgctt catggatatc gcaaataacg atttgccctt cgtggacctc aacggtttct
    25201 ccgcagcctt ttaaatattc acggtcagtt tcatcacgat aatattccac tacctgtaat
    25261 gtctctttcg gcgcatattc aattttttgc tgcccttgca ggtagtaatg cacttcaaaa
    25321 tagcgtcgat gcccggtaaa tagcgcatcg gtcgcggagt ctgtctccac gcggtaagtc
    25381 aatgagtcac cgatggagtg ggcgacgcca ggctgaatgt tgtcgatatt ttcaatcgct
    25441 tcaacgcagc gttgccactt cttgccagag gcgtaaatct ggcggaactg ttctaagtta
    25501 tcgatgatcc tcattgctta ttctcgctgt gcaaattcgt ggaaaagaac cctgcgccga
    25561 actcatacga cgccaggctt tgcgcggtag cttctccgcc agaaaccggc agcaacgtaa
    25621 agccgtagct gaagtcacgg aaccagacgc gccaggagtc cagcacctcg ctgccccagg
    25681 agttggagcc gaggccaagc agctggtgat cgaggttcag ggtgatgtca tcactgcgct
    25741 gcagctcgtt acagtgctgg gcagcgtgga tgttttcctg ggtatagtgc caggcgctga
    25801 agttaattgg gcgctgcgga accaccagca aaccgttgcc gtggcggtta gtcagtgccg
    25861 tccagcggac atgctgacgg ttgccgttgt tctgcgggaa gggatagttc tcgaacatgg
    25921 catcgacggt gctgcgccag atatcgatga tgttagcctg ctggctgtcg gcgtagtttt
    25981 cgcccggtcc acgaccgtaa tacgccacct gatcgtattc gccgttaatc cccatggtga
    26041 aaccgatgca cggaatgatg tgcggatagt cgccgtaacg ctcgccggaa agcgccacgt
    26101 taacctggcc atcggcagcg atgcgccaga tgtaggtgca gcgcatcccg aagtcaaaca
    26161 ccggcggggc aataactgtg cggctgatga tcaacacttc accatcgctc tgttctacgg
    26221 caaagtcgcg cagatgttcc tgcatgatct gcaaatgatt cggttgccac agcccttcgt
    26281 actcctgctt gtggttgtcg atcattggct tgaagaagtt gatctttggc tcgcgggtca
    26341 gcagcgactc gccattcacc tgccaggatg tcggtttgcc actcattttt gagaaggtga
    26401 tcgcgaagtt gtagccgcga acggtgcagc tcaaacgatc gtcttccagc gtcagcggac
    26461 gcgcattatt tggtgcgaaa ggcactggtt gcgcggtgtt ttccttcagc gggaactgat
    26521 aagtggcgat agaatgcccg gcttcgctgt agcgggtgcg ggaatctttg gtcaccgtaa
    26581 tgttgaggaa cgcttcgcgg gcgtccagct gcggcagcgt gatctgcaag ggggcttcgc
    26641 tgttcggcgc aacgtcgcgc agtttaatct gctgcgtcgc gagcgtttca ccttcggcgc
    26701 gcacctctgc gtgcagggtg tagtcatcaa gcgtggtaaa ccacagttta ttttcgactt
    26761 tcagctcgcc gcgagtcaga tccagcgcgt ggatttttac cggcgcgata acctgtttgt
    26821 actctttcag gcctggtccc ggcgtctgat cggaatagat caaaccatca agacagaagt
    26881 tatagttgtt gggatagtcg ccgtagtcgc tgccgaattt ataccagaca ttgccgttgt
    26941 catcctgcgc ctggatcccg tggtcgcacc actcccaaac ataatgtccc tgaatacaat
    27001 cgtgcttata gaagacgttc tggtactccg tcagcccgcc cggtccgttc cccatcgcat
    27061 gagcatattc acagatgatg cgcggcttcg gatgcgggta ttcaccaaac tcattcatca
    27121 gcggcacgcg ggtgtacatg gtggaaataa tatcgaccac ttcagcatcg cgatcttctt
    27181 cgtaatgcac cagtcgcgtg tcatccagcg ccttcgctgc gtggtacatc gcgcggatgt
    27241 tacagccata gccggattca ttgcccagcg accagatgat gatcgacgga tggtttttct
    27301 gcgcgtgaat atggcgaaca atgcgctcga cgtagacttt ttcccactgc ggatcgtcgg
    27361 taatacggct gatatcgccg acattagcaa agccgtgcga ttcgacgtcg gtttccgcca
    27421 tcacaaacaa gccgtagata tcacacagtt cgtaaaaacg cggatcgttc gggtagtgag
    27481 cggtacgcac ggagttgatg ttgtgctgct tcatcaactg gagatctttc tcgacgcgat
    27541 ccattccaac ggcgcggcct ttgcgatgat cgttgtcgtg acggttgacg ccgtgcagca
    27601 tcacataacg gttattgatc cagaacagac cgtcgcgcac tttgatatca cggaagccaa
    27661 cgcgttgtgg taccacttcc agaacgttgc cgttggcgtc tttcagcgtc atgaccagat
    27721 ggtaaagata aggggattct gctgaccatt gctgcggctg ttcgacagta aaagcaaagc
    27781 tggcgctggt cagtttttca attgccaaat gatcaatggc gctgctgtgc accacgcgtt
    27841 cgccatcaaa cagggtatat tccagcgtcg tgacgacagg ggaggcggag agattttcca
    27901 gcaccacttc gcaggaaagc gtggcatcgc aataggcttc gtcaaagtcg gtacgaacgg
    27961 tgaaatcgtt aatatgtgtt agttgttttc cgatcagata aacatcgcgg aagatccccg
    28021 ctgaccacca catatcctgg tcttccacgt aggtagagtc cgcccattgc atcacgcgca
    28081 cacacaacag gttgtcgccg gttttaacca tcgcgctgat gtcaaactct gcggtcaggc
    28141 gactgccctt gctgaaaccc acatactgac cgttaacgta gacttcaaaa taggtttcga
    28201 cgccgtcaaa tttaatcagc gtctgtttac cctgccagcc gtcgctgagg gtgaaaatac
    28261 gttgataggc accggttggg ttatcgctgg ggacaaacgg cacatcgatg gggaacggaa
    28321 aaccttcgtc ggtatattgc agtttgccgt gaccttccat ttgccacatg gcggggacgg
    28381 taatatgccc ccagtcagcc attaactcag aggtgaaggc ttcaggcact tgcagcggat
    28441 ggtcaaaaaa gtggaaattc cactggccgc ttaagggcag aaacaggctg ctggtttcgc
    28501 gggcaaaggt acgcgcttgc gcaacagaat catatgaaaa aaagtacgca cgtggcgcaa
    28561 gtcggttttc gtgggtgagc tgaatgtttt cccagcgatt catagggcct cccggtagag
    28621 agaaatcgga accattcatg gtgatggtag tttgtgtaat tgctggggag tttagtaaaa
    28681 tttttactaa aaagtagcgg gtaaatgcat ttactttaac tggatcacga aaataaacgg
    28741 cagcactgtc cggcaaaaac gattgccgga cagcgtgcaa ggggatttaa cgggtcgtgc
    28801 cgcgcagttt taatttgctg ggaacgaaga ccaacagcgg cagcgcgcga ccatcgcggg
    28861 ctttttcata caccaggtta acgccctgac ttcccatcat ctcggaatgg atgcgcacgg
    28921 tggagagcgg cggaaaggta aatcgcgcgg tggggatatc gttaacgctg ataagcgaaa
    28981 tatcctgtgg gatgttcagg ccacgttcat gaattgcccg cagtacgccg atagcaatgg
    29041 aatcagaagc aacaaacagt gccttcggat agtcttcctg cgccagcagc tgttttgcca
    29101 gttcatagcc cgacgaactg gaaaagcctc cgcgccagat atcctcttcg cgtaccactt
    29161 gtttcagtcg gccatattcc gcaaaggcga cctcacgaat atccgctttg cctggctcat
    29221 cttcaccgcc aataaaacca atacgattaa cgccctgatt gatatagaag tcgatgattt
    29281 ctttactgat gcgtgccaga tcgatatcca ccgcatcgta accgctgccg ggttcgtgaa
    29341 agtcgataaa acagatattg tcggtcaacg cacaggcggc ggcgcgcagg gcgggcgtgg
    29401 gtttgccgac aattaaaata ccggtgacgt ttttaatgtc tggtaatccg ctgtgttcat
    29461 aacagttggt gagctcaatg cccagctttt cgcactgagt ttcaatgccg tggcggatcg
    29521 ccagatagta aggatcgttg atctccagct cctgttggta gctgtagata gccagaatat
    29581 ggtgttggtt gactgcgcct gtctggagtt tacgggcact actggtcttg tactccagct
    29641 tttcggcgat ctcgagaatg cgatgtttcg tctcttcttt cacattcaat gtcggatcgt
    29701 catttaagac cctggatact gtcgccaggg atacgccagc ttcgattgcg atgtctttta
    29761 gtgttgccat gtttaccttt tttactttcc ctgatgtgcg cccccattgt agaggaagtg
    29821 cttatcgggt cacctgtttt cagtaaataa agcgcgtttc tttgacgctg taacggtaac
    29881 acagcggctc acgaatgtga tcacgcctgc attctccatt tttagcctac aggattgaga
    29941 aaaacttttg ctatagttta gtaaaatttt acttgaaggg aggaagcaat ggaaaccgtg
    30001 gcttacgctg attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc
    30061 catgaaaacg ccgacaagct gtacatcgta caggttgatg tgggggaaaa aacactgcaa
    30121 accgtgacca gcctggtgcc gtactacagc gaagaagagc tgatggggaa aacggtggtg
    30181 gtcttatgca atctgcaaaa agcgaaaatg cgtggtgaaa cgtcggaatg catgctgttg
    30241 tgtgcggaaa ccgatgacgg cagtgaaagc gtgttgttaa cgccggagcg gatgatgcct
    30301 gcgggcgtac gcgtcgtata aaaagatcgg atggcgacgt cgtatcgcca tccgatttga
    30361 tattacgctt cttcgacact tactcgcatg gccgccagcg ccttacgcgc cgctttgatc
    30421 accagttcac actgttcaat ggtcagtgtc agtggcggtt caatgcggat cgttttggcg
    30481 ttattgagcg ttccggccac cagtacgcgc tggcggaaca tctcgctggc aaagttatag
    30541 ccgatttcgt tatcaacaaa ctcaattgcc atcaacatcc ctttaccacg cgcttcctgt
    30601 accagatcgg gatattcccg cgccagttga cggaaaccgt ccagcaacat atcgcctttt
    30661 tgctcagcct gagccggtaa gttctgctcc agcaacacat tgatggtcgc cagcgccgcc
    30721 gcacaggcca gcgggttgcc gccaaaggtg gtggtatgca ggaatgggtt gtcgaacaga
    30781 actgaaaaca cctcttcagt ggcgatggtc gcgccaatcg gcatcacgcc gccgccgagc
    30841 gctttggcaa ggcagaggat atccggctgt acgttctcat gctcgcaggc gaacatcttg
    30901 cccgtgcgcc ccatgcccgt ttgtacttca tcgaggatca tcagtgcgcc gaactcatcg
    30961 catagcttac gtacggcggt gagatagccc ggcggcggca gaattacgcc accttcaccc
    31021 tgaatcggtt cgaggatcac cgcagccaca tcatcaccgg tttttttgca ctcgttaaga
    31081 gccgtgcgca tggcttcgat attgccaaac ggcacatgac ggaagcccgg cagtaacggc
    31141 ataaacggtt tgcggaaggt cgatttcgcc gtggccgaca gcgcgccaag tgatttaccg
    31201 tggaacgcgc cgctggtggc aataaaagta aacttgccgc gcggtgactg gtaagctttc
    31261 gccagcttca gcgctgcttc aacggactcg gtgccgctat tacagaagaa gctgtatttc
    31321 agtttaccgg gcgttagcgc agcaagggtt ttcgccaaca tcgcccgtaa cggatcgagc
    31381 agctcctggc tgtgcagcgg ttgtttcgca agttgattct gtacggcgga aaccacaact
    31441 ggattacggt gccccacgtt gaaaattcca aaacctccca ggcagtcgat aaactcctgt
    31501 ccctgggtgt cgacaagcgt atttaaacct cccgcttgcc actctacggc tccgtaatcc
    31561 ccgccggcgg taacagattt gcgatactct aaaaaccccg gattgacatg ctctttgaag
    31621 tattcaatca cctctcggtt aagtgctttc atctcctcat gatccagcgt tcgcttctca
    31681 atgagattca gggcgtgggc gctgcacgct aaagccgatg cgctcgaagg taacctgttc
    31741 aaaatatgct ccggaggctc gcgtatcaca tgataccgat ttaagtattg cagggattgc
    31801 gccactccgg ctgtgatcgc gaaaatcggg ataaacacaa atgtggttat catttgcgca
    31861 atatttaatg ctatttgtta acatctgttg ttatttcgca cggtttttgt cgggtatgga
    31921 tttgatgatt gctgcgcaaa tgcacgtttt cagggcaata attgcctgat ttttgggcaa
    31981 gataattaac gttttcgtat agtaacagag tgaaagtatt ttaaatcaaa gaattagatt
    32041 tgtgctttaa tcgcaaattg cgatctaaat caaattaatc ggttaaagat aaccgcagcg
    32101 gggccgacat aaactctgac aagaagttaa caaccatata acctgcacag gacgcgaaca
    32161 tgtcttctca tccgtatgtc acccagcaaa ataccccgct ggcgggcgat accactctga
    32221 tgtccactac cgatctgcaa agctatatca ctcatgctaa tgacactttt gtgcaggtga
    32281 gcggctttac cttgcaagag ttacaagggc agccgcacaa catggtgcgt cacccggata
    32341 tgccaaaagc ggcgtttgcg gatatgtggt tcaccctgaa aaaaggggag ccctggagcg
    32401 gcatcgtgaa aaatcgccgc aaaaatggtg accattattg ggtgcgggcc aatgcggtac
    32461 cgatggtgcg cgagggaaaa atcagtggct atatgtcgat tcgtacccgg gcgacggatg
    32521 aagagatcgc ggcggtggag ccgctgtaca aagcgctgaa cgccggacgt accggtaagc
    32581 gtattcataa aggcctggtg gtgcgtaaag gctggctggg taaactgcct tcattaccgc
    32641 ttcgctggcg ggcgcgtgga gtgatgaccc tgatgtttat cttgctggcg gccatgcttt
    32701 ggtttgttgc tgccccggtg gtgacgtata tcctctgtgc gttagtgatg ttgttggcaa
    32761 gcgcctgttt tgaatggcag attgtccgcc cgatagaaaa tgtcgcccgt caggcactga
    32821 aggtggcgac cggagagcgt aatagtgttg agcatctgaa tcgcagcgat gagctggggc
    32881 tgacattacg cgcggttggg cagcttggcc tgatgtgccg ttggctaatt aacgatgtct
    32941 caagccaggt gtccagtgtc agaaatggca gtgagacgct ggcgaaaggc accgatgaac
    33001 tgaacgaaca tacccagcag acagttgata acgttcagca aacggtggcg accatgaacc
    33061 aaatggcggc gtcggtgaaa cagaactctg ccacggcgtc ggctgccgat aaactttcta
    33121 tcaccgccag taatgcggca gtgcagggcg gggaggcgat gactacggtg atcaagacaa
    33181 tggacgatat tgccgacagt acccagcgca ttggcaccat tacttcgctg attaacgata
    33241 ttgcgtttca gaccaatatt ctggccctga atgcggcggt ggaagcggcg cgtgccggcg
    33301 aacagggcaa aggttttgca gtggtggcgg gggaagtgcg tcatttagcc agccgcagcg
    33361 ctaatgctgc caacgatatt cgcaagctga ttgatgccag tgctgataag gtgcaatccg
    33421 gttcgcagca ggtacatgcc gccggacgga cgatggaaga tattgtggca caggtgaaaa
    33481 acgtcaccca gttgatcgcc cagattagcc attcaacgct ggaacaggcc gatgggcttt
    33541 ccagcctgac ccgtgcagtg gatgagctta acctgatcac ccagaaaaat gccgagctgg
    33601 tggaagagag tgcgcaggtg tcggcgatgg tgaaacaccg cgccagccga ctggaagacg
    33661 cggtgacggt gctgcattaa tcggtacgcc ggatccggct gttggagcac aatgcctgat
    33721 gcgatgctgg cgcatcttat caggcctacg gggtgtgtag cgggtgtagg ccggataagg
    33781 cgcgtcaagc gtcgcatccg gcaattgcac cgcgccactg gcggatgcgg cgattaatcc
    33841 agttgcgtaa tatcaaaagc ggcgcggtcg atcaccgcaa tgatcttttt tatttgtgca
    33901 tcagtgatat cgctctggtt gacgcgtaaa tccagcaccg ctttaaaatt atccagcgcc
    33961 cgcttcattt gcgggttctg gcgcagcgcc gcgccaacgc aacgcgcttt gatgcgttct
    34021 tcaatcatct ccacctgttc gcggttttct tccagccact gcgcgccttg ttcggtcagt
    34081 gcaatctgct tcttaccacc ttcctcttcg cggatggtaa tcagcgactg ctcctgcaga
    34141 aaatccagcg tcgggtagat gacgcccggg cttggggtgt aattcccctg ggttagattc
    34201 tcaatcgctt taatcaattc gtaaccgtgg ctgtcatcgc gcgagagaat atccagaatc
    34261 accagacgca attcaccgtg accaaagaaa cgttgccgac gaccgccgcc gcgaccgtgg
    34321 cgaccaccgc agcattgacc gtgttcatgc tggtgaccgt gtccgcagtg ctcgtgttct
    34381 gacttctcac ctttgcagca gccctcatgg cgtggctggc cttcatgttt acaacaccct
    34441 tcgtgatgat ggctcatatc agcc
//
LOCUS       DBANUU010000040        34417 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000040 DBANUU010000000
VERSION     DBANUU010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.040"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            249..386
                     /locus_tag="ACIE68_001841"
                     /pseudo
     CDS             249..386
                     /locus_tag="ACIE68_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(494..1729)
                     /gene="aslB"
                     /locus_tag="ACIE68_001842"
     CDS             complement(494..1729)
                     /gene="aslB"
                     /locus_tag="ACIE68_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFX5908167.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDKFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVSK"
     gene            complement(1876..1952)
                     /locus_tag="ACIE68_001843"
     tRNA            complement(1876..1952)
                     /locus_tag="ACIE68_001843"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1916..1918),aa:Pro,seq:tgg)
     gene            complement(1995..2081)
                     /locus_tag="ACIE68_001844"
     tRNA            complement(1995..2081)
                     /locus_tag="ACIE68_001844"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2045..2047),aa:Leu,seq:cag)
     gene            complement(2102..2177)
                     /locus_tag="ACIE68_001845"
     tRNA            complement(2102..2177)
                     /locus_tag="ACIE68_001845"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2142..2144),aa:His,seq:gtg)
     gene            complement(2236..2312)
                     /locus_tag="ACIE68_001846"
     tRNA            complement(2236..2312)
                     /locus_tag="ACIE68_001846"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2276..2278),aa:Arg,seq:ccg)
     gene            complement(2415..3800)
                     /gene="yifK"
                     /locus_tag="ACIE68_001847"
     CDS             complement(2415..3800)
                     /gene="yifK"
                     /locus_tag="ACIE68_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFX5908168.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3991..4731)
                     /gene="wecG"
                     /locus_tag="ACIE68_001848"
     CDS             complement(3991..4731)
                     /gene="wecG"
                     /locus_tag="ACIE68_001848"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFX5908169.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4734..6086)
                     /gene="wzyE"
                     /locus_tag="ACIE68_001849"
     CDS             complement(4734..6086)
                     /gene="wzyE"
                     /locus_tag="ACIE68_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFX5908170.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(6083..7162)
                     /gene="rffT"
                     /locus_tag="ACIE68_001850"
                     /gene_synonym="wecF"
     CDS             complement(6083..7162)
                     /gene="rffT"
                     /locus_tag="ACIE68_001850"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFX5908171.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAIIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGYVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVLEAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(7159..8409)
                     /gene="wzxE"
                     /locus_tag="ACIE68_001851"
     CDS             complement(7159..8409)
                     /gene="wzxE"
                     /locus_tag="ACIE68_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFX5908172.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8411..9541)
                     /gene="rffA"
                     /locus_tag="ACIE68_001852"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8411..9541)
                     /gene="rffA"
                     /locus_tag="ACIE68_001852"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFX5908173.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9546..10220)
                     /gene="rffC"
                     /locus_tag="ACIE68_001853"
                     /gene_synonym="wecD"
     CDS             complement(9546..10220)
                     /gene="rffC"
                     /locus_tag="ACIE68_001853"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFX5908174.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10198..11079)
                     /gene="rfbA"
                     /locus_tag="ACIE68_001854"
     CDS             complement(10198..11079)
                     /gene="rfbA"
                     /locus_tag="ACIE68_001854"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFX5908175.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11098..12165)
                     /gene="rffG"
                     /locus_tag="ACIE68_001855"
     CDS             complement(11098..12165)
                     /gene="rffG"
                     /locus_tag="ACIE68_001855"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFX5908176.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12165..13427)
                     /gene="wecC"
                     /locus_tag="ACIE68_001856"
     CDS             complement(12165..13427)
                     /gene="wecC"
                     /locus_tag="ACIE68_001856"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFX5908177.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13424..14554)
                     /gene="wecB"
                     /locus_tag="ACIE68_001857"
     CDS             complement(13424..14554)
                     /gene="wecB"
                     /locus_tag="ACIE68_001857"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFX5908178.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRLELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14610..15656)
                     /gene="wzzE"
                     /locus_tag="ACIE68_001858"
     CDS             complement(14610..15656)
                     /gene="wzzE"
                     /locus_tag="ACIE68_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFX5908179.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15668..16771)
                     /gene="wecA"
                     /locus_tag="ACIE68_001859"
     CDS             complement(15668..16771)
                     /gene="wecA"
                     /locus_tag="ACIE68_001859"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFX5908180.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(17011..18270)
                     /gene="rho"
                     /locus_tag="ACIE68_001860"
     CDS             complement(17011..18270)
                     /gene="rho"
                     /locus_tag="ACIE68_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFX5908181.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18355..18456)
                     /gene="rhoL"
                     /locus_tag="ACIE68_001861"
     CDS             complement(18355..18456)
                     /gene="rhoL"
                     /locus_tag="ACIE68_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFX5908182.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18597..18926)
                     /gene="trxA"
                     /locus_tag="ACIE68_001862"
     CDS             complement(18597..18926)
                     /gene="trxA"
                     /locus_tag="ACIE68_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFX5908183.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            19057..20322
                     /gene="rhlB"
                     /locus_tag="ACIE68_001863"
     CDS             19057..20322
                     /gene="rhlB"
                     /locus_tag="ACIE68_001863"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFX5908184.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20456..21940
                     /gene="gppA"
                     /locus_tag="ACIE68_001864"
     CDS             20456..21940
                     /gene="gppA"
                     /locus_tag="ACIE68_001864"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFX5908185.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWYLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21987..24008)
                     /gene="rep"
                     /locus_tag="ACIE68_001865"
     CDS             complement(21987..24008)
                     /gene="rep"
                     /locus_tag="ACIE68_001865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFX5908186.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24225..24674
                     /locus_tag="ACIE68_001866"
     CDS             24225..24674
                     /locus_tag="ACIE68_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFX5908187.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVIPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            24873..25154
                     /gene="ppiC"
                     /locus_tag="ACIE68_001867"
     CDS             24873..25154
                     /gene="ppiC"
                     /locus_tag="ACIE68_001867"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFX5908188.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25241..26716)
                     /gene="ilvC"
                     /locus_tag="ACIE68_001868"
     CDS             complement(25241..26716)
                     /gene="ilvC"
                     /locus_tag="ACIE68_001868"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFX5908189.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            26866..27759
                     /gene="ilvY"
                     /locus_tag="ACIE68_001869"
     CDS             26866..27759
                     /gene="ilvY"
                     /locus_tag="ACIE68_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFX5908190.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(27811..29355)
                     /gene="ilvA"
                     /locus_tag="ACIE68_001870"
     CDS             complement(27811..29355)
                     /gene="ilvA"
                     /locus_tag="ACIE68_001870"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFX5908191.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29358..31208)
                     /gene="ilvD"
                     /locus_tag="ACIE68_001871"
     CDS             complement(29358..31208)
                     /gene="ilvD"
                     /locus_tag="ACIE68_001871"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFX5908192.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31273..32202)
                     /gene="ilvE"
                     /locus_tag="ACIE68_001872"
     CDS             complement(31273..32202)
                     /gene="ilvE"
                     /locus_tag="ACIE68_001872"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFX5908193.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32222..32485)
                     /gene="ilvM"
                     /locus_tag="ACIE68_001873"
     CDS             complement(32222..32485)
                     /gene="ilvM"
                     /locus_tag="ACIE68_001873"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFX5908194.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32482..34128)
                     /gene="ilvG"
                     /locus_tag="ACIE68_001874"
     CDS             complement(32482..34128)
                     /gene="ilvG"
                     /locus_tag="ACIE68_001874"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFX5908195.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQLHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34131..34181)
                     /gene="ilvX"
                     /locus_tag="ACIE68_001875"
     CDS             complement(34131..34181)
                     /gene="ilvX"
                     /locus_tag="ACIE68_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFX5908196.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34268..34366)
                     /gene="ilvL"
                     /locus_tag="ACIE68_001876"
     CDS             complement(34268..34366)
                     /gene="ilvL"
                     /locus_tag="ACIE68_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFX5908197.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         8344 a         9488 c         8682 g         7903 t
ORIGIN      
        1 attaattgcc gttaaaacta aaaaaagcat caataatcaa cgcgatataa taaacctgcc
       61 ttacatatca actgcgccag aggtaggatt gaaaacgctc tcctgatttt ccaattcatt
      121 ttctggataa ataaataatt tatttttgtc actattattt atgtaatcat cctgtcaggg
      181 agagggatct caattatcaa tgcttaatta cgtcatcatt ttgatgacat gaaaccggag
      241 cattttgcat ggaattttcg ttttcaccca aacttcttgt tgttgctgtc gccgccgctc
      301 ttcctctcat ggccagcgca gcagataccc cgtcaactgc caccgcacgc aaaggctttg
      361 ccggatacga tcacccaaac cagtatccac agaacgctgc aatttattga atttgcacga
      421 tcatgtaggc cggataaggc gtttacgccg catccggcaa tcaaccgcag gcggccacaa
      481 aatggccgct tttttactta ctcaccacca gcaaatgtgc ctgcataatg tcactggccg
      541 ggcgaccgtg cgccagcaaa tcagccattg ctttaagata tggcggtaga tggcggaaat
      601 aacgctgata cccggcacac aaataattca gccccggttt gccgctggca tcgagcatga
      661 agcggtgttt cgggcagcct ccccagcacg cttttaacac gttacaactg cgacactgcg
      721 ccggtaattg cttaaattta tcttcaccaa acacctgctg ttgcggggaa tcgatcattt
      781 ctgcaattgt ttgctgatgc atattcccca gccgatattg cggataaaca tagtgatcgc
      841 aggcgtaaac gtcgccgttg tgctcaacaa tcaccgagcg cccacaggtt ggctgatgat
      901 ggcaaaccgc acccggcgca ccgacaaaat tggcaaacgc ccattcgata ttcatcacga
      961 aaatcttgcc gacgtcgcgt ttgatccagt ggtcgaatat cgccaccaga aattcaccaa
     1021 atttatcggg gcgcaccgac cattccgtca gttccccctg aatatcacca ggcgcatgca
     1081 atttcagtcc atcgcgggca gctgtttcat cagccagacg ctcgaccacc ggaataaact
     1141 ggatgaattc gactcccgca tcgcacaaaa aatcataaac ctgcaacggt tgctgcgcgc
     1201 tggtgcggtt gacgcagacc agcacgttat agtcgacatg atgtttttgc agcagcgtca
     1261 gggcacgcat caccagctta tgcgttggtc tgccaccttt ggtcacgcga tattgattgt
     1321 ggatctcagc cggaccgtcc agcgataacc caacaagaaa atgattttct gccagaaatg
     1381 cacaccattt atcatcgagc agcacgccgt tagtctggaa actgttactt atcttcctgc
     1441 cagcaccgta tttcgcctgt aactttacgg cacggcggta aaaatccagc ccgagtaacg
     1501 ttggttcgcc gccctgccag gtaaaagcca cttcgttttg cggttcgctg gcagcgatat
     1561 agtgacggac atacgcttcc agcgtgtcat cgtccatatg cgtgactggc ttttcgcggt
     1621 aaagggattg tttttcgaga taaaaacagt agtcacagtt cagattacaa tcggaaccac
     1681 tcggtttcgc catcacatga aaagcacgcg ttggaacctg ttgcagcatg gtcactcctt
     1741 gcctgaagtc atccttcaat acgttaaggg cgaaatcata ccgtggggca atgagttatt
     1801 acgggcgttt attgcgaagg gagggtgacg aaatgcacag aaaacaaaaa accccgccga
     1861 agcggggttc aaaattggtc ggcgagagag gattcgaacc tccgacccac tggtcccaaa
     1921 ccagttgcgc taccaagctg cgctactcgc cgaaatactg ctttttgaat ttttagttca
     1981 attctttaaa gtcgtggtgc gagggggggg acttgaaccc ccacgtccgt aaggacacta
     2041 acacctgaag ctagcgcgtc taccaattcc gccaccttcg cattgtcaca acttctaata
     2101 atggggtggc taatgggatt cgaacccacg acaactggaa tcacaatcca gggctctacc
     2161 aactgagcta tagccaccac tacaaatctt gttacgcggt attactacca ccgcagctca
     2221 agcgccggga ctaaatggcg cgcccgacag gattcgaacc tgagacctct gcctccggag
     2281 ggcagcgctc tatccagctg agctacgggc gcttagcgcc gttgcggggg tggataatac
     2341 ggacttccca cccctctgtc tagtcccttt ttaaataaat tgcgcgtttg gttacggttt
     2401 gtgcgttttg ctgcttattc ctccagttta tgcgctttcc cgtggcgatt aaggccaaaa
     2461 actttataaa tcgccgtcac cgccagcata aagatgatgc caacaaacag cgacatacgc
     2521 gtatcttcat taaagtacat gccgatcaaa acgcaaatca ggaatgccat tgttacgtag
     2581 ttggcccaag ggaacaggat tgagcggaac ggatggctgg caatcgccgc tttatgtgca
     2641 cgccgaaaac gcagctggct tatcaatatc acaaaccatg gcaccatccc cggaagcacg
     2701 ctggcactgt agacgtagac aaacacacgc tgcggattgg gaatgatgta gttcaggcat
     2761 gagccaatta gcagaatagc aatagatact gccacacccg caactggtac gccgtgacgg
     2821 gaaactttcg ccatcgccgc cggtaactga cggtttttcg ccagtgcgta gagcatacgt
     2881 ccgcaactgt acatgccgct gttacagcca gagagcgcag ccgtcagcac cacaaagttg
     2941 ataatgcccg ccgctgcggt aataccgatt ttggcaaaag tcagtacgaa cgggctgccg
     3001 ttgctgccaa tttcattcca cgggaagatg gtgacgataa cgaaaatcgc gcctacgtag
     3061 aaaatcagga tccgccacag cactttgcct acggcactgc gcagcgtcac ctgcggattc
     3121 ttcgcttcac cggcagtaat gccaatcagc tccacgccct ggtaggacgc caccacaata
     3181 cacagcgcgg tcaggaaccc tttccagcca cccgcaaaga aaccgccatg ctctgtgaga
     3241 ttgctaaaac caatcgactg cccgccattg ccaaagccaa agaaaatcac gcccaggcca
     3301 atgacaatca tcacgataat cgtggtgact ttgatcatcg cgaaccagaa ctcgatttcg
     3361 ccgtacaacc gcaccgccgc cagattcgcc aacgccacca gcgccactgc gatcaatgcg
     3421 ggtatccact gcgccatctc cgggaaccag aactggacat aaacgccaat ggcggtgatt
     3481 tctgagatcc ccaccgccat ccacataaac caataagacc aggcggtgag ataaccaaag
     3541 aacgggctca tataacgatg cgcataaacg gcgaacgaac cggtaaccgg ttcgaggaac
     3601 aacatttcgc ccattgaacg catgatgaaa aagacgaata gcccggcgat gatataggcc
     3661 aacaatacgg atggcccggc ccatttcaag gtactggcgg cccccataaa caggccgacg
     3721 ccaatggtgc cccccagggc gatgagttcg atatgtcgag cttccagccc acgctgtagc
     3781 tctggtttgt tatctgccat aaatcctcgt gttgtgtttg catgctttcc ggtgttaccg
     3841 gttatcgtta tgggtacatc gagtgttgcg aatgttttcg taattcagga gaaatggcaa
     3901 ataaagcatt aaaaatttga atgctttgtg taataaaaaa gcagacaggc gacgaagtga
     3961 ccactccgtc gctttacaaa gagaggaaaa tcagaggttg ccggtgtaat gccagcgtaa
     4021 ataacgcagc aaacgaagct gacgcttaat gcggctcggc tgcgaaagca ggcggtagag
     4081 ccactccagt cccagcgttt gccagatttt cggtgcgcgt tttacgtgac cggtgaaaac
     4141 atcgtaagtc ccgccaacgc ccatatacag cgcatctgga tgcaccagac ggcagtcgcg
     4201 catgaagatc tcctgctttg gcgatcccat cgcaacggtg acgatttgcg ctccgctggc
     4261 atgaatgcgt tcaaacagcg cctgacgctg ctcgggttta aaataaccat cctgactgcc
     4321 aacgatattc acattccatt ggttgcgcag tttagcttca gtttgcgcca gcacttcagg
     4381 tttaccgccc acaagaaata ccggcgtccc ttctttgcct gcgcgcgcca tcagctcttc
     4441 ccagagatcg gcaccggcaa cgcgggaaac ctgcgcctgc gggtactttt tacgtactga
     4501 acgtacaacg ctgatgccat ccgcatattt aaattcggca gcgttaatta actccctgac
     4561 ctcggcgtta tcttcaatgg tcagcatttt ttcagcatta atggcaacca gcgttccctg
     4621 cttaagctgc ccgtcagcaa acagataatc gagggcgtgc tgcatatcac gccaaccaat
     4681 caactgtaag ccacgcagcg tataggtcgg tgccgtggtg ttgttattca ttgttatcct
     4741 tcaacctgcg tccggagcga tgattttgta cgtttatgaa tgagtccggc gctttcaaaa
     4801 agccagtaca acagttttgc gatcatcaga catgcgccga agaccacgat aaaaaagact
     4861 acgcgtgaga caaacgaatc cagcccttca cgtgccagca cgatcatatt gaagatcgcc
     4921 ccaaagcaga aactgtgcaa tatcgcagcc ttatagcgat tagtctcgcg gttgcccagc
     4981 tcatacagcc agtcgaacca tttgatgatc agtccaacca caatcgcccc gagcgggatg
     5041 aacaacgcgc cgcccatcac caccagtgag cctataagcg taggcgagat cgccagtccg
     5101 gagtggttat tcagcacttc ccaggtaaag tagttggctg agttcagcac catactcggg
     5161 cgccccggcc acagccagga agggataaag acatagaaat cgcggacaat tggagccagg
     5221 ccctggaagt cgatgttgtc gtagttctgc aacagcaacg ccagattctc ccacggcgag
     5281 aaggtgtcgc gagtgagata gagaaacgta tagaacgctt catcgccgct cacattcatt
     5341 ccatagcgtt ttaacgccag ccagaacatg ccaacaatcc ccagcacgcc cgccgccgcc
     5401 agcatccaca acgaaatcca gccgcgaata atgccaataa acaggaagat agcgaatgcg
     5461 atgatgatat tggcgcgagt gccgccgaca atcatataag tcagcaagcc aaaagcgacc
     5521 gtgctgacga ggaaaaacag ccacgctttg ctgtcctggc gcagaaagta gaccaccagc
     5581 atcgccggga tgaaaaagta aaagaagcgt tttaacgcca cgccggagac ttcactggag
     5641 aagatctgac tgtaggagtt aagccggaac agcaaaaagc cgttgtgcat gaagaagatg
     5701 ccgacgctta ccagcgcgat gcccatcagg atcacccacg tgagattggt ctccacgcgg
     5761 ttcatggtaa acagcggacg gcgcggtaca tcagcaacgc gtttgcgtag gcgggttttg
     5821 taggtgacat agtaaaccgc gtagaagcag cccgcagaaa gcaacgcctg caacaagatt
     5881 tctggcggcg cgacaccaac atcaaagcga aataccagca cgctggtcag cgggaagccg
     5941 aagaaaaagg tgagcaaaaa cagcaatgaa aagaagacat tgaagttaaa gcgcacacgg
     6001 cgaaactcaa accaggtcag cgtggcaata aacagcgtgc agagcagcca gaccacaaac
     6061 aggccactga attgcagcag actcatgcga cctccccggc ggcaatcgcc aacgcccgct
     6121 gccagccttg tagatagtta gggctaaaga aggcaatggt gtttttatcc accgacgcca
     6181 actggcgctg cgcttcaagc acaatatcct cgttgagatc gtcggtagta aacagcaccg
     6241 gcaaatgctg ttccgtcata tcctgccaga acggattttc ccggttaagc acacaaggaa
     6301 tgcccgcctg aatcagtaag cacagcgtac caatgccctg ctggcgggca aaaataaagt
     6361 aaccgagatc gcactgacga agtagcgcca gataggcgtc aaattccagt ttttcgctca
     6421 gaacttgcag attttcttcg ctgaataact ccagccccgc ctgacgaact tcctcaatgt
     6481 acgcttcgtt attaggcgga tatcccatcg gcaccaccac ttttaccgta tcgccaaatt
     6541 gctgatgaac ggcacgtaag gcggcgacat gctcattgct gcggtcgccg gagttcccca
     6601 ccagaatggt cattttccct tcacgttgcc gatcgttcgc catcgtattg agagaagggt
     6661 ccatccgcgt cgggaagtac agcagttcgc cccgcacctt tggatgcgtt ttggcaaaaa
     6721 agctcaaatc accgcgggtg gcaaatacat agccgactcg cttttgcgcc aggcgacgta
     6781 gcgggtaaaa aagcttatat ctcaagccac tggaaagctc atacaggtct gccccccaga
     6841 tatgccagaa aaactggctg ggcttaatgc caccactcag cagagccagc cacagtgtgg
     6901 gattgaactg accgtggaaa aaaaatcgct gctgacggtt agcttttgct ttcgcgatga
     6961 tcgcttccgc cagcgatttt ttcccaggga aaaattgcac agaaagcgcc ggacagctat
     7021 cacttaagcc gtcgtccttg ccaacaacca taaactcgcg cgcgtgctcg ctcgtcgcgg
     7081 ccagcgcgtc attgaaaaac cgcaaaacgg ttcggttatg gtgagggata tccgatccca
     7141 gtacgtgaat cagtacagtc atgcccgcct acgccagagt aaaaacacgc cacaacaaag
     7201 agaaaaatag acgatataag ttgccatata tgcctgcgcc gcgcccagtg caccatgcgc
     7261 agggattagc cagtgggcaa ataccatcaa taaagtgaac tggctgactt ccgccagaat
     7321 ataaaaccgc agtgacgctt tggcgatcac cagataacca aagacataag cgcccacttt
     7381 taacacatca cccactaact gccaggcaaa gagatcgcgc atagcggtaa atttattcga
     7441 caacagcagc cagatagcaa aatcacgcag caaccagacg gtaaaactcg ccgccgccac
     7501 tgccggtaag acgaatttca gcgatttaac cacttcccgg gtgatatcgc gcttttccgt
     7561 tagccgcgac aacgtgggca gcaaatatac gctgaacgat gccgtaataa attgcaggta
     7621 ggcatcggaa atactgctca ccccttgcca gatccccacc tcatcccagc tatactgcgc
     7681 tgccagcagt ttgcgcatca tgatgtaagc aacaggcaaa gtcaccgacg taatcaacgc
     7741 catgagcgta aatttgctca actgccctgc cagaccgtta tcccagctgg gtttcagata
     7801 gcttaacggg atgacaccac gtttgatcaa catgatggcg gcaggaatga ccaccagcgc
     7861 gggaatcagc gccagaccca gcaacgcccc ttcataaccg cccaaacggt aactgacgta
     7921 gtacgcgaga acgccaatca agctgccgac aatcagcgat aacgcattac ctgcggcatc
     7981 gcgaaagcct ttcatcagcg ccagtaacag gttgccccag gcgatcccca tttgcaccag
     8041 cgccactaaa cgcaccagcc cctgatagtc ggtattacca aacaatccct ggctgattgg
     8101 cgcagctgcc agcacaaaaa ccagcgccat cagcgtagag aagccaagta ccatcgctga
     8161 tgaagtgccg accacgcggc gcagctgttg gggattatca tggtactggg caacgtattt
     8221 ggttacaccg ttaaagatgc cagccccggc aagcacgccg agcacggtaa tcaactggcg
     8281 gaaatttgcc gccagcccaa gccccgccgg accaaatgac accgccagca acttaccgac
     8341 cagtaacccg gcaccaatct tgaccagtgt actggccgcc gtccacaagg acgcttttgc
     8401 caacgacata tcaggaaaag tagttcaaca aagtcgcaat taccgtacgc tgattgacgg
     8461 gcgacaggtt gtagaacagc ggcaggcgca gcaggcgctc gctctctttg gtggtgtagc
     8521 gatcttcacc gtggaactca ccaaagtgtt cccccgcagg gcaaccgtgc agcggaatgt
     8581 aatgaaacac cgccatgatt tccgcttctt tcagaaagtt aatcaacgcg ctccggtcat
     8641 caatatcccg cagtttaatg tagaacatat gcgcgttctg cacgcagcca tcgggaatcg
     8701 acggcagctc gatacgcccg gctttcgcca gaggcgctaa cgcatcgtag tagttttgcc
     8761 acagcgccag acgttgctgg ttgatacgat ccgctgcttc cagttgcgcc cacaggtatg
     8821 cagcttgcag atcggacatc aaatagctgg agccaatatc gcgccaggta tatttatcga
     8881 cctgaccacg gaagaactgg ctgcggttag tgcccttttc acggatgatc tcggctcgtt
     8941 cgattaacgc tttatcgtta atcagcgtcg cgccgccttc accgcccgcc gtgtagtttt
     9001 tggtttcatg gaagctaaag cagccaatat gaccaatggt tcccagtgca cgccctttgt
     9061 aagtggacat cacgccctga gcggcatctt ctaccacaaa caaattatgc tttttcgcca
     9121 acgccataat ggtgtccatt tcgcaggcca cacccgcgta atggaccggc acgataacgc
     9181 gcgttttgtc ggtgatcgcc gcttcaatca gcgtttcgtc gatgttcatg gtgtccgggc
     9241 gaacatccac aaaaacgatt tttgcgccac gcagcacaaa ggcattggcg gtggagacaa
     9301 aggtgtagct cggcatgatc acttcatcgc caggctggat atcgagcagc agcgccgcca
     9361 tctccagcga agcggtgcag gacggcgtca gtaacacttt ggcgctgcca aaacgttgct
     9421 ccagccactg ctggcagcga cgggtaaaac cgccatcgcc acacagtttg ccgctaccca
     9481 ttgccgactg catatagtcg agttcggttc ccaccaccgg cggtgcgtta aatggaatca
     9541 tgtgatcacc tgtataacca gtacgcggtg ctttctacat tcgcaccact ttgtatgtat
     9601 cgtttaagcg cggcggtgtt gcccatttgg gtcgccaccc gcaaagttgt tttaccgcga
     9661 gcatacgccc agtttagcgc cgtttgcatc agctcagcac ctgcaccgcg tccagccagc
     9721 aggccaattc gcgcatctgt cgcattgagt tcccgtaaag agacatagcc gcgaatatcg
     9781 ccggacgccg cacgtaaaat cagacattga tgatcaaagg tgccgcgcac ggcattttca
     9841 atccactgtg cataaaagcg actgctggcg tcaggcgcat accacggcgc acgaaaacgg
     9901 ctttgcgcaa atgcggcgct ggctaactga cgtaatgcgg gaatatcggt ctcttgtgcc
     9961 actacagcac cgctatcact ggcattgttc acgggtagcg ccaaatcaac ttcaccttct
    10021 accagggaga atcccagctg ttgcagggca tccagttcac ccgtatttga tgccgcaatt
    10081 ttggcctgca cccgtgacca cggcgctaac gcgtctggcg tcaggagcgg tgcttcagac
    10141 gtaatgcgca cgatggcgct gttaacacca aagaaggcgt tttcccaggt tagtggctca
    10201 atactggcgc ggacgggcac gaagtaactc cagcagatat tggccgtagc cagttttcgc
    10261 taatgaactg gcagcacgct tcacaccctc gtcatcgagc cagccgttac gccaggcgat
    10321 ctcttccagg caggcaatct taaagccctg gcgtttttct actgtctgca caaaggtact
    10381 ggcttcaatc aggctgtcgt gagtgccagt gtccagccag gcaaatccgc gcccgagcag
    10441 ttcaacggtc aggttgcccg cctcgaggta catctggttg atggaggtaa tctccagttc
    10501 accacgctcc gacggcttca cctgctttgc gtactccacg actttactgt cgtagaaata
    10561 aagcccggtc accgcccagt ttgacttcgg ctgttttggc ttttcttcca gcgagatagc
    10621 gcggaaattg tcgtcaaact ccaccacgcc aaagcgttcc gggtccatca cctgatagcc
    10681 aaaaaccgtc gccccttccg tgcgcgccgc aacatgacgc agcttcggac tgaagccctg
    10741 accgaagaaa atgttatcgc ccagcaccag acaagaaggt tcaccgttga ggaaggtttc
    10801 accgatgata aaggcctgcg ccagaccgtc cgggctgggc tgttcggcat attccagctg
    10861 gataccgaac tcactaccat cgcccagcag gcgctggaaa taacctttat cttccggcgt
    10921 agtgatgatg agaatttcgc ggataccggc cagcatcagc accgacagcg gatagtaaat
    10981 cattggctta tcgtaaatcg gcaacagttg cttcgatacg ccgcgcgtaa tcggatgcaa
    11041 tcgggtgccg gaaccgcccg ccaggataat acctttcatg tttgcctccg ctgaaaatta
    11101 gcctttcaga cctaaacgct cgccctgata gctgccgtcc tgcacctgct tccaccagct
    11161 ttcattagcc agataccact gcaccgtttt acgcattcca ctttcaaagg tttcctgtgg
    11221 cagccaacca agttcacggg caattttcga agcatcaatg gcataacgca gatcatgccc
    11281 cggacggtca gcgacaaagg tgatcaagtc acgatagtgc gccacgccgt gcggcttgtt
    11341 cggagccagt tcttccagca gctcgcaaat ggtttccaca acatcgagat tcttacgctc
    11401 gttgtgacca ccaatattat aggtttcacc gactttcccg gtggtcgcca cgcaatacag
    11461 cgcgcgggcg tgatcttcca catacagcca gtcacggatt tgctgcccgt tgccatatac
    11521 cggcagcggt ttacccgcca gcgcgttgag gatcatcagc gggatcagtt tttccggaaa
    11581 gtggtaagga ccgtagttat tcgagcagtt ggtgatcagc gtcggcagac cgtaggtccg
    11641 cagccaggcg cgcaccagat ggtcgctgct ggcttttgac gcggaataag ggctgctcgg
    11701 cgcatacggc gtggtttcgg tgaagaaatc atccgtcgag tgcaggtcac catatacttc
    11761 gtcggtggag atatgatgaa aacggaacgc tgatttttta tcttccgtca gcgtattcca
    11821 gtaagcccgc gccgcttcaa gcaatgtata agtcccgaca atgttggttt caataaacgc
    11881 tgccgggccg tcaatagaac ggtcaacatg gctttcggct gccagatgca tgacacagtc
    11941 tggctgatgc tcagtgaata cgcgtgccag ttctgcccga tcgcagatat caactttctc
    12001 aaaggcaaag cgctcgcttt gcgcgaccgg tgccagcgac atcaggtttc cggcgtaggt
    12061 cagcttatcg accactacca ccgcgtcgct cgtttcgttg atgatataac gcaccagcgc
    12121 cgagccaata aacccggcac cacctgttat cagaattttt ctcatcagcg ccagactcct
    12181 ttggcatcga cgacatactg ctgatggaca ttgtcgccat tgataacttt gaactgacta
    12241 tgatcgacca gcatcaccag cacgtctgcc gttgccagcg cctcgtcaag ctgcgccaga
    12301 gtacaaagcc cggtcagttt tttcggcaac tggtggatgt taggctcaac aaccagagtt
    12361 tcgccgctat gccactgggc gatcagttca gcgatttcca tcgccgggct ttcgcgcagg
    12421 tcatcaatat tcggtttaaa cgccagacca aagcaggcga ttttcagttc actggcgcgt
    12481 ttatcggtag cagccaggca atcagccacc gccgctttca cctgatcgat aacccagaac
    12541 ggtttgtgat cgttcacttc gcgcgcggta cggataagcc gcgcctgctg ggggttctgt
    12601 gccacgataa accacggatc aacagcaatg cagtgaccgc ccacgccagg gccaggctga
    12661 agaatattaa cgcgagggtg acgattcgcc aggcgaatca gttcccagac gttaatcccc
    12721 tgatcggcac aaatcagcga caattcatta gcaaaagcga tattcacatc gcggaagctg
    12781 ttttcggtga gcttacacat ttccgccgtc cgcgagttag tgacgacaca ctcaccttcg
    12841 aggaaaattt tgtacagttc gctggcgcgg gccgaacaaa ccggcgtcat accaccaatc
    12901 acgcgatcgt ttttaatcag ctcgaccata acctggcccg gtaacacgcg ttccgggcag
    12961 taagcaatgt tgacgtccgc ctgctcgccc acctgctgtg ggaaagtgag atccggacgc
    13021 atctctgcta accattctgc catcttctcg gttgacccca ccggcgaggt ggattcaagg
    13081 atcaccagcg cgcctttttt cagcactggc gcaatggagc gagcagccga ttcaacgtag
    13141 gtcatatctg gctcatgatc gcctttaaac ggcgtaggta cagcaatcag ccaggcatcc
    13201 gcttcaactg gcgtcgtgct cgctcgtaaa aaaccgcctt ctacggcagt ttttactacg
    13261 ctcgccaaat caggttcgac gatatggatt tcgccacgat tgatggtatc aaccgcgtgt
    13321 tggttgatat cgacaccaat cacctgtttt tgccgtgagg caaacgcagc tgctgtaggc
    13381 agcccgatgt aacccagtcc gataacagaa atggtcgcaa aactcatagt gatatccgat
    13441 tattttttaa cgcttccaga atgcgagagc atgcctgacc atcaccatac gggttatggg
    13501 cgcggctcat agcttgatat tcgttttcgt cttttaaaag acgcgtcact tcctcgacaa
    13561 ttcgctgctt atccgtgcct accagacgca ccgtacccgc cgtcaccgct tccggacgct
    13621 cagtggtatc gcgcatcacc agcaccggtt tccccagcga aggcgcttct tcctgaatgc
    13681 cgcctgagtc ggtcaaaatc agccaggcgt ggttcatcag ccagacaaac ggtaaatact
    13741 cctggggatc gatcagaatg acatttttca catgccccag aatgcgattg accggctctc
    13801 tgacgttcgg gttgagatgc accggataga caatctggat gtcctggtgc gtggtggcga
    13861 tgtctgccag cgcgtggcag atttcttcaa agccacgacc gaaactttca cgcctgtgac
    13921 cggtcaccag aatcatcttt ttatcggggt cgataaacgg gtaatttgcc gccagttcta
    13981 aacgcagcgt gtcgctgctc atcacctgat cacgcaccca taacagtgca tcaatgactg
    14041 tattaccagt aatgaagatt cggctatccg caacgttttc acgcagcaag ttttgccggg
    14101 aagtttcggt tggagagaag tgatacatcg ccagatgccc ggtcaatgta cggttagcct
    14161 cttccggcca cggcgaatag agatcgcccg tgcgtaaacc agcctcaacg tgaccaacag
    14221 gaatacgctg ataaaacgcc gccaggctgg ttgccagcgt cgttgttgtg tcgccgtgaa
    14281 ccagcacgac gtctggtttg aactcggcaa gaataggttt tagcccttcc agaatccgac
    14341 aggttatctc tgtcaggccc tgtcctggct gcattatgtt gagatcgtag tcaggtacaa
    14401 tggaaaagag tttcagcacc tgatcgagca tctcccgatg ctgcgcagtg acgcaaactt
    14461 tagcctcaaa aaaaggatct tttgccaacg catgcaccaa cggagccatc ttgatggctt
    14521 ctgggcgcgt accaaataca gtcagtactt tcacatcgat tctcttcgaa taagcggcga
    14581 gcgcctttgc gctcgcagca gcagtgttgc tatttcgagc aacggcgggt taatgcgaca
    14641 ccagccccga tcagcccccc gacaatgccc cacataatca tcaggaaggc acgacgtggg
    14701 ctatcgcgtt ttaccggttc ttccggcgta cgcaaatagc gataggtctg aaaacgcgga
    14761 tccagggttg gaccaacatt cagggtgttt aacatggccc gattctgatc atagtcgaga
    14821 tcaaaggccg gaccgacggc ctgtaaattt tccagtcgag cctggagcat tggacgccca
    14881 agcaggaaca tttctgaatc aggtaattcc tcggcaggca catctgtcgc actgcgcgaa
    14941 atattatgct gctcagcaat tttcagcgcc tgctcaatgc tgttcatccg gcggtcgtag
    15001 atggctttcg ccacctcttc ctgacgcttc acctgagctt tcatctggat ggtgcgtgcc
    15061 gcccatgcgc ctttcagctc atcattcaga tggctggctg cacgctggct ggcaaaagca
    15121 acatactgac gtaacaggtt attagcgtca ggcgcggttt cggcaataag cttcacgctg
    15181 tcattgaccg cgcgggtaaa gtctccgggg ataaactgga tgttgttaat catttcatcc
    15241 agcaacgccg catcggcttt gctgttgccc accatccgct gtttgtaata gtcggtttgc
    15301 agccagaact ctctgcgggt atcccacgag gccagttgca taacaaactc tttataggct
    15361 tcgtccatga ccgatggttg atcggcagaa gccatgtttg aacggacatc caggttacgc
    15421 aaaaattgct gctgcgagta atatcccccc agcatattca ccgttggacg atcggtaatc
    15481 gccgtcgagc tccactcctg acgagcaaaa aaagtatacg ccagcgcgat taacgcaaac
    15541 gccagcccca tgccaataat ccatagcttc ccagcccaca aggtacgaaa caacccacga
    15601 atatccagtt cattttcagc gtcttcggcc ggtttcccag gcattggttg tgtcatcaca
    15661 tcctcattta tttggttaaa ttggggctgc caccacgatt tctacgcagt ctgcgtttta
    15721 cgcgcttaat aaagcgagca actttccagg cacgcttaat gcaatatcca tagaggaaga
    15781 atgctagcaa aaagagcacc agcatgaccc actccgggac aaaatgagaa tattctgcca
    15841 gcacgccaat ggaagcgagc agtgctgcgg caagggtaat cagcacaaac gcctgacggg
    15901 aagtaaaccc ggcacgcatg atcaaatggt gaatatgctg acggtcagga gagaatgggc
    15961 tcatgccttt acgcaggcga cggtacataa tcgccaccat atccattagc ggaatggcga
    16021 ttatccacaa agcggtaacc gggctgatgg gatgggtttt gccctgggtc gtttcgagca
    16081 ggatccagat aacggtaaaa ccaatcagcg tactgcccgc atcacccata aagactttgt
    16141 agcggcgacc caggatacca aggttaagca tgatgtatgg caggatggcg gcgatcatcg
    16201 caaagcacca gattgcgagg ctggtttgcc cgtcgaacca caaaatcata ccgattgctg
    16261 caaacgagac gcaggacaac ccgcccagca agccatcaat gccatcaacc atgttgaacg
    16321 cattaatggc cgcccagacg gcaaatagcg tcaggaagta accaaacggt ccgagcacca
    16381 tctcccagga gccaaagata taacccaggc tactgagata aagtttgccg aacaccatca
    16441 taacaatgcc aacagcggcc tgtatggtgg cacggatttt tacgctgata tcaaaacggt
    16501 catccagcgc gccaatgaaa acaagcacac cggcacaagc gagatagaga gatgcatgcg
    16561 gaatatagta atcgacaatt ccgaacgtga agcaaatccc tgcgtaaacc gaaatccccc
    16621 caacgagagg tatcaatccc tggtgacgtt tgcggaagtt tggtttatcc actaaaccga
    16681 ctttttttgc caccttacgg gcaaaaaaca gaaacagtgt cgtgaataaa aaaatactga
    16741 tgagatcagt actcactgtc agtaaattca caatgcatgc tctcagagaa aattattagc
    16801 agaagtataa ccacgaagac ctttattcag aagggaaagt ccgttccgaa cccaccagtc
    16861 ctgtgcaaaa attaaccatt tgatatgcaa attgcataac atgccagcaa attccaggaa
    16921 ttttcctaac atcgccagcg cggcattaag attacagata taaaagcaaa acgccacgta
    16981 aacacgtggc gtttttggca taagacaaat ttatgagcgt ttcatcattt cgaagaaatc
    17041 gtcattggtc ttggtcatgg ccagtttatt aatgaggaat tccattgcat cgatttcgcc
    17101 catcgggtga atgattttgc gcaggatcca cattttctgc agttcttcct gagtcgtgag
    17161 cagctcttct ttacgggtac cggaacggtt gtagtcgata gccgggaaga cgcgtttttc
    17221 agcgatctta cgagagaggt gcagttccat gttgcctgta cctttaaact cttcgtaaat
    17281 aacttcgtcc attttagaac cggtatcgat aagcgccgtc gcgataatgg tcaggctgcc
    17341 gccctcttcc acgttacgcg ccgcaccgaa gaagcgtttc ggacgatgaa gggcattggc
    17401 gtccacacca ccggtcaaca ctttacctga cgccggaaca acagtgttgt aagcgcgcgc
    17461 cagacgagtg atggagtcga gcagaatgat aacgtctttc ttgtgctcaa ccaggcgttt
    17521 ggccttctcg atcaccattt ccgcaacctg aacgtggcga gatgcgggtt cgtcaaaggt
    17581 agaagcaaca acttcacctt ttaccagacg ctgcatctcg gttacttctt ccggacgttc
    17641 gtcgatcagc agaaccatca gcacacaatc cgggtggttg taagcaatgc tctgagcaat
    17701 gttctgcagc agcatagttt taccggcttt cggcggtgcc acaatcagac cacgctgacc
    17761 acgaccgata ggtgatgcca gatccagtac gcgagcggtt aaatcttccg tagaaccgtt
    17821 accacgttcc atacgcagac gagagtttgc gtgcagcggg gttaagttct caaagaggat
    17881 tttgttgcgg gcgttttcag gtttgtcgaa gttaacttcg ttaactttca gcagcgcaaa
    17941 atagcgttca ccttctttcg gcgggcgaat cttaccagag atggtatcac cagtgcggag
    18001 gttgaaacgg cggatttggc taggggaaac gtagatgtca tcaggaccgg cgaggtagga
    18061 gctgtctgcg gaacggagga aaccaaatcc atcctgcaat atctccagta cgccatcacc
    18121 aaagatatct tcgccactct ttgcgtgctg cttcaggatg gcaaaaataa tgtcctgctt
    18181 acgcatacga gccaggtttt ccagccccat attttcgccg agagtgatca gctcagaaac
    18241 cggcgtattc tttaattcgg taagattcat agtggtgtga gttcttaaac ttggggtaaa
    18301 tctcgaactt aatgttgtga atggtatggc aggatcatcc atgcctgttt acggtcatcg
    18361 actcatgtct tttcgctgcc tggtcacagg agagtacgca gaactgaaac gacaagacgg
    18421 attgagtgac gagccagaaa tctgttcact tcgcatttaa gataaaacgg gaagcgttgg
    18481 aaattacaag attcaaactt aataaggtat gtttaatacg aagtcaacac taagttagca
    18541 tgactcacgc cgggcgtcca gtttttagcg acggggcacc cgaacatgaa attcccttac
    18601 gccaggttag cgtcgaggaa ctctttcaac tgacctttag acagtgcacc cactttggtt
    18661 gccgccactt caccgttttt gaacagcagc agagtcggga taccacggat gccatatttc
    18721 ggcgcagtgc cagggttttg atcgatgttc agttttgcaa cggtcagttt gccctgatat
    18781 tcgtcagcga tttcatccag aatcggggcg atcattttgc acggaccgca ccactctgcc
    18841 cagaaatcga cgaggatcgc cccgtccgct ttgagtacat ccgtgtcaaa actgtcgtca
    18901 gtcaggtgaa taattttatc gctcatatat aactccacag gaataagcct ggcgtgttgg
    18961 tttcgttgtt ggtgtaacat taaccaacta aaggttgact ttatttcacc ggatacgctt
    19021 tcgtaaagca atagtaagct gatattctac cacactatga gcaaaacaca tttaacagaa
    19081 cagaagtttt ccgacttcgc cctgcatccg aaggttgtag aagcccttga aaaaaaaggg
    19141 tttcataact gtacgcccat tcaggcactg gcccttccgc tgacgctggc gggtcgtgac
    19201 gtagccgggc aggcgcaaac cggtaccggg aaaacgatgg cgtttcttac gtcaacgttt
    19261 cattatcttc tctctcatcc tgcgattgcc gatcgcaagg tgaatcaacc acgtgcctta
    19321 attatggcac cgacgcgtga acttgccgtg cagatccatg ccgacgcaga accgctggcg
    19381 gaagctaccg gcctgaagct gggtctggct tacggtggtg atggctacga caaacagctg
    19441 aaagtgctgg aaagcggcgt tgacattctg attggcacta cggggcgttt aattgactac
    19501 gccaagcaga accacattaa cctcggtgcc attcaggtgg tggtactgga cgaagccgat
    19561 cgcatgtacg atctgggctt tattaaagat atccgctggc tgttccgccg tatgccgcct
    19621 gcaaaccagc gcctcaacat gctgttctcc gccacgcttt cgtaccgggt acgtgaactg
    19681 gcgttcgagc agatgaacaa tgccgaatat attgaagtgg aaccggaaca gaaaacgggc
    19741 caccgtatta aagaagagct tttctaccct tctaacgaag aaaaaatgcg tttgctgcaa
    19801 acgctgatcg aagaagagtg gccagacaga gcgattattt tcgccaatac caaacaccgt
    19861 tgtgaagaga tctggggcca cctggcggca gatggtcatc gtgtcggttt gttgacaggc
    19921 gatgtcgcac agaaaaaacg tctgcgtatt cttgatgaat ttacccgtgg cgatctggat
    19981 attctggttg ccaccgacgt tgccgcgcgt ggtttgcata ttccggcagt gacgcacgtc
    20041 tttaactacg atttacccga tgactgtgaa gattacgttc accgtattgg tcgtacaggt
    20101 cgcgcaggcg caagcggtca ctctatcagc ctggcgtgtg aagagtatgc attgaatttg
    20161 cctgctattg agacctatat tggtcactca attccggtaa gcaaatacaa tccggacgca
    20221 ttgatgaccg atctgccaaa accgctgcgc ctcacgcgcc cacgtacagg caatggtccg
    20281 cgtcgtactg gcgctccgcg taatcgtcgt cgttcaggtt aaataaaatg tcatactcaa
    20341 aatcattcaa actgtatcaa gatggcaaga gaatgaatcc cgatgagcat actgtagtaa
    20401 gtgattcagg tgaaagaatg cagccaacac agagacagat tgaaggatga agagtatggg
    20461 ttccacctcg tcgctgtatg cagccattga tctcggttcg aatagttttc atatgctggt
    20521 tgtgcgcgag gtggctggaa gcatccagac gctgacgcga ataaaacgca aagtgcgtct
    20581 ggctgctggc ctgaatagcg aaaatgccct gtctaatgaa gcaatggagc gcggttggca
    20641 atgtctgcgc ctgtttgctg aacgtctgca agatatccct ccctcgcaaa ttcgcgttgt
    20701 cgctacggcg acattacgtc ttgccgtcaa tgcgggtgat tttattgcca aagcgcagga
    20761 aatcctcggt tgtccggtac aggtaatcag cggtgaagag gaagcacgtc tgatttatca
    20821 gggcgttgct cacacaactg gcggtgccga tcagcgcctg gtggtggata taggcggtgc
    20881 cagtactgaa ctggtaaccg gcacgggtgc acaaaccacc tcgttgttca gcctgtcgat
    20941 gggctgcgtc acctggctgg aacgctattt tgccgatcgt aatctggggc aggaaaattt
    21001 tgatgctgca gaaaaagcgg cacgcgaagt gttacgtccg gttgccgatg aattacggta
    21061 tcacggctgg aaagtgtgcg ttggtgcttc cggcaccgtg caggcgttac aggaaatcat
    21121 gatggcacag gggatggatg aacgcattac cctggaaaag ttgcagcaat tgaaacagcg
    21181 agccattcat tgcggtcggc tggaggaact ggagattgac gggctgacgc tggaacgtgc
    21241 gttagtgttc ccgagtggtc tggcgatcct gatcgccatt tttaccgaac tgaatattca
    21301 gtgtatgacc ctggcgggcg gtgcgctgcg tgaaggcctg gtctacggca tgttgcatct
    21361 ggccgtcgag caggatattc gcagccgtac gctgcgtaat attcagcgcc gctttatgat
    21421 cgatattgat caggcgcagc gcgtagccaa agttgcagct aacttcttcg atcaggtgga
    21481 aaacgaatgg tatcttgaag caataagccg cgatttgctc atcagcgctt gtcagcttca
    21541 tgaaatcggc ctgagcgttg acttcaaaca agcgccgcaa cacgctgctt atctggtgcg
    21601 taacctggat cttcccggtt ttacccctgc acagaaaaaa ctgctggcga cgctactgct
    21661 caaccagact aacccggtcg atctctcatc gctgcatcag caaaatgccg taccaccgcg
    21721 cgtcgcagaa caactctgcc gtttactccg cctggccatc atttttgcca gccgtcgtcg
    21781 tgacgattta gtgccagaga tgacattaca ggctaaccat gaactgttga ccttgacgct
    21841 tccgcaaggt tggctaaccc aacatccgct gggtaaagag attattgctc aggaaagcca
    21901 gtggcagagc tatgtccact ggccgctgga agtgcattaa tgagtaagtg ccggatgcga
    21961 tgctgacgca tcttttccgg ccttgattat ttccctcgtt ttgccgccat catcgctttc
    22021 agattcgcca gatggctttg ccctttctgc atccgttctt cggcgctgac cactttgcgc
    22081 tcctgttccc aaatcagatc atcctgcggc agctccagca aaaagcggct cggctccggg
    22141 cgcaccagct cgccgtactg acggcgttct ttacacagcg taaaggtcaa ttccttctgg
    22201 gcgcgggtaa tgccgacata ggccagccgc cgctcctcat cgatattatc ttcatcgatg
    22261 ctgctctggt gcggcaaaaa cccttcttcc ataccgacca tgtagacata aggaaactcc
    22321 agcccttttg acgcgtgcag agtcatcagt tgcacctgat ccagctcttc ttcactctca
    22381 ccacgctcca tcatgtcgcg caaagtaaag cgcgtcacca cctgggtgag cgtcatcggc
    22441 tcatccagtt cactgccttc cagcatctcc gtcatccagc taaacagttg gttgacgttc
    22501 ttcatgcgca tttcggcggc tttcgggctg ggcgatgttt cgtacagcca ggattcataa
    22561 tccatgccat ggatcagatc acgcaccgca gcaatcggct cccgctccgc caggcgctgg
    22621 atttctgcca accagtgagt gaagcgggtc aatgcttcat aaccacgtcc gctaagcgtc
    22681 tgactcaggc ccatatcaaa gctggcggta aacatgcttt tattgcgcgt catcgcccac
    22741 tcacccagct ttttcagcgt cgccgggcca atctctcgct tcggcgtgtt aacgatacgc
    22801 agaaatgcgc tgtcatcgtc cgggttagtc agcacgcgca gataagccag caagtccttg
    22861 atttcaggac gagagaaaaa cgacgtacca ccagaaattt tgtacgggat gcggttttgc
    22921 atcaggaatt tttcaaacac ccgcgactga tggttaccgc gataaagaat ggcgtaatct
    22981 ttgtactgcg ttttattgac gaagtgatgg gcgatcagct cgccagtaac gcgctcggct
    23041 tcatgttcct cgttattcgc gcttaaaact tttagctctg tgccgtaacc cagttcggag
    23101 aacagacgct tttcaaagac gtgcggatta ttggcgatca ggatgttcgc cgctttcaaa
    23161 atacgtccgg aagagcggta gttctgctca agcttaatca ccttcagcgc cggaaaatcc
    23221 tgactcagca gcaccaggtt ttgtggacgt gcgccgcgcc aggagtagat cgactggtcg
    23281 tcatcgccca ccacggtaaa gcgcgcgcgg ctgcccacca gcagtttcac cagctcatac
    23341 tggctggtgt tggtgtcctg atactcatcc accagcagat agcgaatttt gttctgccag
    23401 cgctcgcgga cttcttcatt gcgttgcagc agcaacgtcg gcaataaaat cagatcatcg
    23461 aagtcgagaa cgttacaggc tttcaggtgt gcatcataca gcccataaca atgggcaaaa
    23521 atacggtccc gctcgccaat cgcactggct gccgcctggg acggtgtttt gagatcgttc
    23581 ttccagttag agatggtcga aatcaactgt tgcaggagaa ctttgtcatc ttcaatcagc
    23641 ccttcggtca actctttgag caacgccaac tgatcggtat cgtcaaacag cgaaaagttc
    23701 gccttcatcc ccagcgccgc atactcacgc ttgatgatat ccagccccag cgtgtggaag
    23761 gtggagatca tcagcccacg cgcctctttg cgacccagcg tctgcccgac acgctctttc
    23821 atctcgcgcg ccgccttatt ggtaaaggtc accgccgcga tatgtcgcgc ctgataaccg
    23881 caaccgcgga tcagatgagc gattttattg gtgataacac gagttttacc ggaacccgcg
    23941 cccgccagca ccaggcaggg gccggtaacg aattcgacag cttgttgttg gccggggttt
    24001 agacgcatag gtgtattgct caatcttcga acggggggag gattgtagca gaatcgcatt
    24061 tgaatgtgac cgcgtgtaag ccgctaaccg gttggctgcg gatgtttaaa tcagagagag
    24121 aagaccgaaa ccggagttca tcgtgaatcc attagactta gaaaatatcg ggtcgtaacg
    24181 ttgtgttatt actttttcag agtttactaa ggagaggtgt actcgtggcg attaatttca
    24241 gtcctaaggt tggtgaaata ctggaatgca actttggtaa ctatcctgtt agccagaatg
    24301 gacagttcag caccacgtat tacgatggcc gcattcctcc tgaaatgata aaaaacaggt
    24361 tagttgtcgt gctcaacgga aaaattaatg gcaacgcatg tatcgtcatt ccattatcaa
    24421 ccactcgcga tcatgacaag ttaaatcgtg gaatgcatgt tgaaatagcc agtaatgtta
    24481 tcaatggcct gcaattcttt gatcagcaga ttcgttgggc caaagccgac ctggtacaac
    24541 aagtcagtcg aaaccgatta aatagagcaa ggacttacag agggtatctt aatcaatgtt
    24601 tgccacatga gttggttgca gacatacaga gggcggtcat caagtcaatc aatgccatat
    24661 cgctcataaa ttgacaataa aatcaccatc cccgataatg actttgtagc ccatagaggc
    24721 cctaagtttt ctccccttga gggacctgcg aagcccggat tttccgggct tttgccatat
    24781 atgtaccagc ccctccctga ctgacaatta cgttagcatc tgcggatcat gctaaaatcg
    24841 ccgcctgaca attattcact caaggcacga tcatggcaaa aacagcagca gcactgcata
    24901 tccttgtaaa agaagagaaa ctggctctgg atcttctcga gcagattaag aacggtgccg
    24961 atttcggcaa gctggcgaag aaacactcca tttgcccatc aggcaaacgc ggcggtgatt
    25021 taggtgaatt ccgccagggt cagatggttc cggcgttcga taaagtggtt ttctcttgtc
    25081 cggtactgga gccgaccggc ccgctgcaca cccagttcgg atatcacatc attaaggtgc
    25141 tgtaccgcaa ctaatagcaa ggccttctcc aggagaaggc cttgagtgtt ttctccctct
    25201 ccctgtggga gagggtcggg gtgagggcat cagcgcgcac ttaacccgca acagcaatac
    25261 gtttcatatc tgtcatatag ccgcgcagtt tcttacctac ctgctcaatc gcatggctgc
    25321 gaatcgcttc gttcacatca cgcagttgcg cgttatctac cgcgccttcc ggaatagctt
    25381 tacccaagtc gcccggttgc agctctgcca taaacggttt cagcaacggc acacaagcgt
    25441 aagagaacag atagttaccg tactcagcgg tatcagagat aaccacgttc atttcgtaca
    25501 gacgcttacg ggcgatggtg ttggcaatca gcggcagctc gtgcagtgat tcataatatg
    25561 cagactcttc aatgatgccg gaatcgacca tggtttcgaa cgccagttca acgcccgctt
    25621 tcaccatcgc gatcatcagt acgcctttat cgaagtactc ctgctcgccg attttgcctt
    25681 catactgcgg cgcggtttcg aaggcggttt tgccggtctc ttcacgccag gtcagcagtt
    25741 tcttatcatc gttggcccag tccgccatca taccggagga gaattcgcca gagatgatgt
    25801 cgtccatatg tttctggaac aggggtgcca tgatctcttt cagctgttca gacagcgcgt
    25861 aagcacgcag tttcgccggg ttagagagac ggtccatcat cagggtgatg ccgccctgct
    25921 tcagcgcttc ggtgatggtt tcccaaccga actgaattag tttttctgcg tatgccggat
    25981 cggtgccctc ttccaccagc ttgtcgaagc acagcagcga gccagcctgc aacataccgc
    26041 acaggatggt ttgctcaccc atcaggtcag atttcacttc cgcaacgaag gacgattcca
    26101 gcacgcccgc acggtgacca ccggttgctg ccgcccatgc tttagcgatc gccatacctt
    26161 cgcctttcgg atcgttttcc gggtgaacgg caatcagcgt cggtacgccg aatccacgtt
    26221 tgtactcttc acgcacttcg gtgccagggc atttcggcgc aaccatgact acggtgatat
    26281 ctttacggat ctgctcgccc acttcgacga tgttgaaacc gtgcgagtaa cccagcgccg
    26341 cgccgtcttt catcagtggc tgtacggtgc gcactacatc agagtgctgc ttgtccggcg
    26401 tcaggttaac caccagatcc gcctgcggga tcagttcttc gtaagtacca actttaaaac
    26461 cgttttcggt cgctttacgc caggacgcgc gcttctcggc aatcgcttct ttacgcagag
    26521 cgtaggagat atcgagacca gaatcacgca tgttcaggcc ctggttcagc ccctgtgcgc
    26581 cacagccgac gataaccact tttttaccct gaaggtagct cgcgccgtcg gcgaattcgt
    26641 cgcgccccat aaagcgacat ttgcccagct gtgccagctg ctggcgcagg ttcagtgtat
    26701 tgaagtagtt agccatggtg attcctcgtg atgttgtgct tcttattgtt cggttcgcgt
    26761 ttgcgagatt gaattcacta tatgacagga aatttattgc ggaaattgat atattcacaa
    26821 cgtcacattg caatttctgc aacgtcaaca ttgagggctg cgccattgga tttacgcgat
    26881 ctgaaaacct tcctgcatct ggcggaaagc cgccattttg gccgcagcgc gcgggcgatg
    26941 cacgttagcc catccacgct ctcacggcag attcagcgcc tggaagaaga tcttggtcag
    27001 ccgctatttg tgcgcgataa ccgcacggta acgctgaccg aagcgggcga agagttacgc
    27061 gttttcgccc agcaaacatt gttgcagtat cagcaactgc gccacaccat cgatcagcaa
    27121 gggccgtcgc tctctggcga gttacatatt ttctgctcgg tgaccgccgc ttacagccat
    27181 ctaccgccga ttctcgaccg cttccgcgcg gaacacccgt cggtggagat caaactcacc
    27241 actggtgatg cggcagatgc aatggaaaaa gtggtcacgg gagaagcgga tctggcgatt
    27301 gccgggaaac cggagacgct acccggcgct gtggcgtttt cgatgctgga gaatctggca
    27361 gtagtgctga ttgcccccgc gctgccctgc ccggtgcgta atcaggtatc tgtcgaaaag
    27421 cccgactggt caacggtgcc gtttattatg gccgatcagg ggccggtacg ccgccgcatt
    27481 gaactgtggt ttcgacgcaa taaaatcagc aacccgatga tttacgccac ggttggcggg
    27541 catgaagcga tggtatcgat ggtggcgctc ggctgtggtg tggcgctgtt gccggaagtg
    27601 gtgctggaaa acagcccgga gcctgtacgc aaccgggtaa tgattttaga tcgcagcgat
    27661 gagaaaacgc cgtttgaact gggcgtgtgt gcacaaaaaa agcggctgca tgagccgctg
    27721 attgaggcgt tctggaggat tttgccgaac cacaaatgac gttgtcgcgc gggtaggcct
    27781 gataagcgaa gcgctatcag gcatttttcc ctaacccgcc aaaaagaacc tgaacgccgg
    27841 attattggtt tcgtcgtggc aatcgtagcc cagctcattt agccgggttt cgaaatccgg
    27901 ttcatggtcg ccgagttcga acgccgccag tacgcgcccg tagtcggtgc catggctgcg
    27961 atagtggaac aaagaaatgt tccagtgcgt acccagcgtg ttgaggaagc gcagcagcgc
    28021 gcccggtgat tccgggaatt cgaagctgta gaggcgttcc tgcaacggat gtgacggacg
    28081 cccgccgacc atatagcgca catgcaactt cgccatttcg tcgtcggaga gatccaccac
    28141 gctgtagccg ccgtcgttga gcatctgcaa aatttctttg cgctcttcga ggccgcggct
    28201 caggcgcacg ccgacaaaga tgcaggcgtt tttggcatcg gcaaaacggt agttgaactc
    28261 ggtgaccgat cgcccgccaa gcagttggca gaatttgagg aagctgcctt tttcttccgg
    28321 aatggtcacc gccaacaacg cttcacgctg ttcgcccagt tcgcagcgtt ccgagacgta
    28381 gcgcaggccg tggaagttca cgttggcacc ggaaagaata tgcgccagcc gttcgccgcg
    28441 aatgttgtgc tgggcgatat attttttcat tcccgccagc gccagcgcgc cagagggttc
    28501 cgccaccgcg cgcacatctt cgaataaatc cttcatcgcc gcacagatcg catcactatc
    28561 gacggtgatg atgtcgtcga gatactcctg gcataaacgg aaggtttcgt caccgatgcg
    28621 ttttaccgct acgccttcag caaatagccc tacgcgcggc agatcaaccg gatgacccgc
    28681 atccagcgct gctttcaggc aggcggagtc ttccgcttct acggcgatca ctttgatttg
    28741 tggcatcagt tgtttgatca gcaccgcaac gcccgccgcc agaccgccgc cgccgactgg
    28801 cacaaatacg cggtcgagat gggcgtcctg ctggagcagt tccagcgcca gcgtgccttg
    28861 cccggcaatc accatcggat ggtcgaacgg cggcacccag gtgaacccct gctgctgtga
    28921 cagttcgatc gctttagctt tcgcttcgtc aaagttcgcg ccatgaagca gcacttcgcc
    28981 gccgaatcca cgtaccgcat cgactttgat gtcggcggtg gcggttggca taacgatcag
    29041 ggccttcacg cctaaccgcg cagaagaaaa cgcgacgccc tgcgcgtggt tacccgcaga
    29101 agcagtgatc acgccgtgcg ctttttgttc ttccgtcagg cccgccatca tggcgtatgc
    29161 gccgcgcagc ttaaagctgt gcactggctg gcgatcttcg cgcttcacca gaatgacgtt
    29221 atcaagacgc gacgacagtt tttccatttt ttgtagcggc gtaacctgcg ccgcctcgta
    29281 aaccggcgcg cgcagcactg ctcttaaata ttcggcacct tccggagcac cggacagggg
    29341 ttgcgagtca gccattatta accccccagt ttcgatttat cgcgcaccgc acctttgtcg
    29401 gcgctggttg ccaggctggc gtaggcgcgc agggcaaagg aaacctgacg ttcacggttt
    29461 ttcggcgtcc aggctttgtc accccgagct tcctgcgctt cacgacgcgc cgccagttcg
    29521 gcatcgctta cctgtaactg aatgccacgg ttcgggatgt cgatagcgat caggtcacca
    29581 tcttcaatca gaccaatgct gccgccgctt gccgcttccg gtgagacgtg gccgatggaa
    29641 agaccagagg tgccgccaga gaaacgaccg tcggtgatca gcgcacaggc tttaccgaga
    29701 cccattgatt tcaggaagct ggttgggtag agcatttcct gcatgccagg gccgcctttc
    29761 gggccttcat agcggatcac caccacatcg cccgcgacaa ctttgccacc gaggatcgct
    29821 tctaccgcgt cgtcctggct ttcgtacact ttcgccgggc cggtgaattt gaggatgctg
    29881 tcatcgacgc ccgcggtttt aacgatgcag ccgttttctg cgaaattacc gtagagcacc
    29941 gccaggccgc cgtctttgct gtaggcgtgt tccagcgagc ggatacagcc attggcgcga
    30001 tcgtcatcga gagaatccca acggcaatcc tgcgagaatg cctgtgtggt acgaatgcct
    30061 gcaggacctg cgcggaacat attttttacc gcgtcatcct gggtcagcat aacgtcgtat
    30121 tgttccagcg tttgcggcaa cgtcaggcca agtacgtttt tcacatcacg gttcagtaac
    30181 cccgcgcgat ccagttcgcc gagaataccg ataacaccac cagcacggtg aacatcttcc
    30241 atatggtatt tctgggtgct cggcgcaact ttacacagct gtggaacctt gcgggaaagc
    30301 ttatcgatat cactcatggt gaagtcgatt tccgcttcct gcgccgccgc cagcaggtga
    30361 agtacggtgt tagtcgatcc acccatcgcg atatccagcg tcatggcgtt ttcaaacgcc
    30421 gccttactgg cgatattacg cggcagtgca ctttcgtcgt tttgctcgta ataacgtttg
    30481 gtcaattcaa caatgcgttt accagcatta aggaacagct gcttacggtc ggcgtgggtt
    30541 gccagcagcg agccgttgcc cggctgcgac aggcccagcg cttcggtcag gcagttcatt
    30601 gagttagcgg taaacatccc ggagcaggaa ccgcaggttg ggcaggcgga acgttcaacc
    30661 tgatcgctct gggagtcaga aactttcggg tctgcgccct ggatcatcgc atcaaccaga
    30721 tcgagtttga ttatctgatc ggacagtttg gttttcccgg cctccatcgg gccgccggaa
    30781 acaaagatca ccggaatatt caggcgcagg gaagccatca gcatccccgg ggtgattttg
    30841 tcgcagttag agatgcagac catggcgtcg gcgcagtggg cgttgaccat atactcaacg
    30901 gaatcagcga tcagttcgcg agatggcagt gaataaagca tccccccgtg gcccatggca
    30961 atcccatcat ccaccgcaat ggtgttgaac tctttggcaa cgccgccagc cgcttcaatt
    31021 tgttcggcga ccagtttacc gagatcgcgc agatggacgt gacccggtac aaattgggtg
    31081 aacgagttca caaccgcgat aatcggctta ccgaaatcgg cgtcggtcat tccggtggcg
    31141 cgccacagcg cacgagcacc cgccatatta cgaccatgag tggtggtggc ggaacggtac
    31201 ttaggcatac tttatttact cccagtgtct gtctcgtaaa tgggacggtg cgtgccgtcc
    31261 cattttttgt atttattgat taacttgatc taaccagccc catttatctt cggtttcgcc
    31321 agtgaagagg ccgaagaagg cttgctgaat gcgtttggta accgggccac aacggccttc
    31381 gccaacctga ataccgtcta cgctgcgcac tggcgtgatt tctgccgccg taccggacat
    31441 aaacacttca tccgccaggt acagggattc gcgcgacagc acctgctcac gtacttcaat
    31501 tcccagctct ttcgccagtt tgatgatggc atcacgggta atacccggca gcgcggagga
    31561 ggtgaacggt ggggtgaaca gcacaccatc tttcacttca aacaggtttt cgcctgcgcc
    31621 ttcagagata taaccgttca catccagcgc gataccttcc tgataaccgt ggcggcgcgc
    31681 ttcgctaccc accagcaggg aagagaggta gttaccaccg gcttttgccg ccgtcgggat
    31741 ggtgtttggt gctgcgcggt tccaggagga aaccatcgca tcgatcccct gctccagcgc
    31801 ttctgcgccc agatacgctc cccacgggaa agcagcgata atcacgtcgg ttgagtatcc
    31861 cgctggcggg tttactccca tgccaacatc accgacgaag atcagcggac ggatataggc
    31921 gctggtgaga ttatttttgc ggatcacgtc acgacaagct tccatcagct catcaatgct
    31981 ctgcgaaacc gggaagcgat agattttggc ggagtcatgc agacgctgca tatgctcacg
    32041 atggcggaat acaaccggtc ctttgtgcga gtcgtagcaa cggatgcctt caaaaaccga
    32101 agtgccatag tgcagcgcgt gcgacatcac atgcaccttc gcgtcttccc agcgaaccat
    32161 ctccccattg aaccaaatgt aatcagcttt cttcgtggtc atttttatat tccttttgcg
    32221 ctcaggcgcg gatttgttgt gatgtggttg tgctctggca gatggcaacg tgtgcgacgt
    32281 ccaccagttt atttaactga ctaaacagta agtcgaccga ccgtgggctg gcaacggtca
    32341 attcgatatt tatattttgt gcatcgctgg cggcggccat attcattgag cagacgtgga
    32401 aaccacgatg acgcaccacg cgtaaaacac gttctaaggt ttccggattg aagcgagccg
    32461 atacattgac ctgatgttgc atcatgataa tttctccaac atttctgaat tactggcacc
    32521 aggcggcacc agcggccaga cgttctcaag ttcgtcgatt gagacatgaa gcaggtatgg
    32581 cccatcactg ttcagcatgg tgtcgagtgc cgcttcaacc tggtctttac gggtgatgtg
    32641 ttggccaggg atgccgaagg cgctggctaa catgagaaaa tcggggttat cggtaagggt
    32701 ggtttcgcta tatcgttcct ggaaaaacag ttgctgccat tgtcgaacca tccctaaccg
    32761 ttggttatcg agtaagacga ttttcaacgg taactgctta cgttttacgg tgcccagctc
    32821 ttgtacattc atcatgaaag agccgtcacc ggagatacag acgacggtat cgttcggtcg
    32881 cgcgacttgc gcgccaaccg ccgccggtaa accgaaaccc atggtgccta agccgctgga
    32941 ggtgatgaaa ttttccgggc gagtatgggc gatgtgctgc gcagcccaca tctggtgctg
    33001 ccccacatct gtggtcacga cgcaatccgc aggtttacga tccgacagtt gttttaacaa
    33061 caatggcgcg tagatagcgt caccgggatg gtcgtaacgc caggcatgtt catcacgcag
    33121 ctgcgcgcag tgtagctgcc agtcattgat atttaacggc tgctgtaatg ctggtaacag
    33181 agcatttaaa tcaccttgta atgccacatg tgcctgacgc agcttgttca tttctgccgg
    33241 gtcgatatcc atatggataa cactggcgtg tggtgcgaag gtgttcagtt tgccggtcac
    33301 ccggtcatca aaacgtgcac ccacggcgat cagcaagtcg cactcctgca ccgcgaagtt
    33361 cgccgctttg gtgccatgca ttcccagcat gcccagatag tacggataat ctgcttcaac
    33421 tgcgcccagc cctttcagcg tgcaggtggc aggcattttt gtggtagcga gaaattctcg
    33481 taaagcagga actgcctgcg ccatacccac gccaccacca acgtacagca tcggtttttg
    33541 cgcttttgcc agcatctggc gcgcttgctc gacttcggca tgtgggaaag tcacttcgtt
    33601 ttcaacggtg gtgaaccacg gttccaggtc accgctggct agctggatat cttttgggat
    33661 atcgaccaga accggaccag gacgacctga gctggcaacg tcgaatgctt cagccatgat
    33721 gcgcggcaat tcttccagcg actgcaccag aaagctgtgc ttggtacagg ctaacgacaa
    33781 tcccaggaca tccacttcct gaaatgcgtc agtgccgata aacggtgcgg acacttgacc
    33841 ggtgatggca acaacaggga tagaatctaa cagtgcgtcc gcaagcccgg ttatcaggtt
    33901 ggttgcgccc ggaccagacg tggcgataca tacgccagtt ttgccggtag cacgggcata
    33961 accgatagcc gccattgccg caccctgctc atgtcggcac agcaagtgct ccacgccgcc
    34021 gtcatacaat gcatcgtaaa ccggcataat tgcgccaccc ggataaccga aaacggtgtt
    34081 cacaccctgt gcccgcaacg catgtaccac ccactgtgcg ccattcatag ttagttcccc
    34141 gtcctgaatc ttgagaaaca gaattttgtg ctgttattca ttgtctgctc ctcggttatg
    34201 tttttaaggt caaaaaaaac ccccggacct ttcggtgcgg gggtcttagt tcgttaaggc
    34261 ttgatctcta agcctttcct cgtccaagtg cagccccgca cggtgggata ataatcacca
    34321 ccacgctaat cacgaccagg ctaatcactc gtagaagggc tgtcattttg tcttttcttg
    34381 catcttgttc gaaggaatgc ctaaagagtt accatag
//
LOCUS       DBANUU010000041        33653 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000041 DBANUU010000000
VERSION     DBANUU010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.041"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            12..980
                     /locus_tag="ACIE68_001877"
     CDS             12..980
                     /locus_tag="ACIE68_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFX5908198.1"
                     /translation="MKDQITHPPDNTDHSVAKQKFRITNWSTYNKALINRGSLTFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFALMNVP
                     LRCPDYTSVSKRAKSVNVSFKTSTRGEIAHLVIDSTGLKVFGEGEWKVRKHGKERRRI
                     WRKLHLAVDSNTHEVVCADLSLNNVTDSEAFPGLIRQTHRKIRAAAADGAYDTRLCHD
                     ELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRLSGSNARWKWTTEYNRRSIAETA
                     MYRMKQLLGDSLTLRDYDGQVAEAMAMVRALNRMTKAGMPESVRIA"
     gene            1335..2612
                     /gene="waaA"
                     /locus_tag="ACIE68_001878"
     CDS             1335..2612
                     /gene="waaA"
                     /locus_tag="ACIE68_001878"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFX5908199.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            2620..3099
                     /gene="coaD"
                     /locus_tag="ACIE68_001879"
     CDS             2620..3099
                     /gene="coaD"
                     /locus_tag="ACIE68_001879"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFX5908200.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(3138..3947)
                     /gene="mutM"
                     /locus_tag="ACIE68_001880"
     CDS             complement(3138..3947)
                     /gene="mutM"
                     /locus_tag="ACIE68_001880"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFX5908201.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(4045..4212)
                     /gene="rpmG"
                     /locus_tag="ACIE68_001881"
     CDS             complement(4045..4212)
                     /gene="rpmG"
                     /locus_tag="ACIE68_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFX5908202.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(4233..4469)
                     /gene="rpmB"
                     /locus_tag="ACIE68_001882"
     CDS             complement(4233..4469)
                     /gene="rpmB"
                     /locus_tag="ACIE68_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFX5908203.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(4686..5354)
                     /gene="radC"
                     /locus_tag="ACIE68_001883"
     CDS             complement(4686..5354)
                     /gene="radC"
                     /locus_tag="ACIE68_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFX5908204.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            5526..6746
                     /gene="coaBC"
                     /locus_tag="ACIE68_001884"
     CDS             5526..6746
                     /gene="coaBC"
                     /locus_tag="ACIE68_001884"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFX5908205.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPARVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDEFTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            6724..7182
                     /gene="dut"
                     /locus_tag="ACIE68_001885"
     CDS             6724..7182
                     /gene="dut"
                     /locus_tag="ACIE68_001885"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFX5908206.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            7289..7885
                     /gene="slmA"
                     /locus_tag="ACIE68_001886"
     CDS             7289..7885
                     /gene="slmA"
                     /locus_tag="ACIE68_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFX5908207.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(7931..8890)
                     /locus_tag="ACIE68_001887"
     CDS             complement(7931..8890)
                     /locus_tag="ACIE68_001887"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HFX5908208.1"
                     /translation="MDATRTTLLALDLFGSPGWSADKEIQRLHALSNHAGRHYRRIIL
                     SKRHGGQRLVLAPDYLLKTVQRNILKNVLSQFPLSPFATAYRPGCPIVSNAQPHCQQP
                     QILKLDIENFFDSISWLQVWRVFRQAQLPRNVVTMLTWICCYNDALPQGAPTSPAISN
                     LVMRRFDERIGEWCQARGITYTRYCDDMTFSGHFNARQVKNKVCGLLAELGLSLNKRK
                     GCLIAACKRQQVTGIVVNHKPQLAREARRALRQEVHLCQKYGVISHLSHRGELDPSGD
                     LHAQATAYLYALQGRINWLLQINPEDEAFQQARESVKRMLVAW"
     gene            complement(9188..9829)
                     /gene="pyrE"
                     /locus_tag="ACIE68_001888"
     CDS             complement(9188..9829)
                     /gene="pyrE"
                     /locus_tag="ACIE68_001888"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFX5908209.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(9895..10611)
                     /gene="rph"
                     /locus_tag="ACIE68_001889"
     CDS             complement(9895..10611)
                     /gene="rph"
                     /locus_tag="ACIE68_001889"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFX5908210.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            10738..11601
                     /gene="yicC"
                     /locus_tag="ACIE68_001890"
     CDS             10738..11601
                     /gene="yicC"
                     /locus_tag="ACIE68_001890"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFX5908211.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            11822..12424
                     /gene="dinD"
                     /locus_tag="ACIE68_001891"
     CDS             11822..12424
                     /gene="dinD"
                     /locus_tag="ACIE68_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFX5908212.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQANTTHFD
                     VGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            12715..13332
                     /gene="yicG"
                     /locus_tag="ACIE68_001892"
     CDS             12715..13332
                     /gene="yicG"
                     /locus_tag="ACIE68_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFX5908213.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(13329..15011)
                     /gene="ligB"
                     /locus_tag="ACIE68_001893"
     CDS             complement(13329..15011)
                     /gene="ligB"
                     /locus_tag="ACIE68_001893"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFX5908214.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAELPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            15269..15892
                     /gene="gmk"
                     /locus_tag="ACIE68_001894"
     CDS             15269..15892
                     /gene="gmk"
                     /locus_tag="ACIE68_001894"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFX5908215.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            15947..16222
                     /gene="rpoZ"
                     /locus_tag="ACIE68_001895"
     CDS             15947..16222
                     /gene="rpoZ"
                     /locus_tag="ACIE68_001895"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFX5908216.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            16241..18349
                     /gene="spoT"
                     /locus_tag="ACIE68_001896"
     CDS             16241..18349
                     /gene="spoT"
                     /locus_tag="ACIE68_001896"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFX5908217.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            18356..19045
                     /gene="trmH"
                     /locus_tag="ACIE68_001897"
     CDS             18356..19045
                     /gene="trmH"
                     /locus_tag="ACIE68_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFX5908218.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            19051..21132
                     /gene="recG"
                     /locus_tag="ACIE68_001898"
     CDS             19051..21132
                     /gene="recG"
                     /locus_tag="ACIE68_001898"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFX5908219.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELQLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(21117..21983)
                     /locus_tag="ACIE68_001899"
     CDS             complement(21117..21983)
                     /locus_tag="ACIE68_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908220.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(21986..23191)
                     /gene="gltS"
                     /locus_tag="ACIE68_001900"
     CDS             complement(21986..23191)
                     /gene="gltS"
                     /locus_tag="ACIE68_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFX5908221.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            23471..24862
                     /gene="xanP"
                     /locus_tag="ACIE68_001901"
     CDS             23471..24862
                     /gene="xanP"
                     /locus_tag="ACIE68_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFX5908222.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVLSRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            24983..26692
                     /gene="yicH"
                     /locus_tag="ACIE68_001902"
     CDS             24983..26692
                     /gene="yicH"
                     /locus_tag="ACIE68_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFX5908223.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPKL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(26745..29063)
                     /gene="yicI"
                     /locus_tag="ACIE68_001903"
     CDS             complement(26745..29063)
                     /gene="yicI"
                     /locus_tag="ACIE68_001903"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFX5908224.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPATDGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(29073..30455)
                     /gene="yicJ"
                     /locus_tag="ACIE68_001904"
     CDS             complement(29073..30455)
                     /gene="yicJ"
                     /locus_tag="ACIE68_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFX5908225.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            30748..30842
                     /locus_tag="ACIE68_001905"
     tRNA            30748..30842
                     /locus_tag="ACIE68_001905"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30782..30784,aa:Sec,seq:tca)
     gene            31141..32331
                     /locus_tag="ACIE68_001906"
     CDS             31141..32331
                     /locus_tag="ACIE68_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFX5908226.1"
                     /translation="MALTDIKVKTAKPKEKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGVVKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLACALATPKKVHLPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIALFKQLEPITKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNAIRGTYNHAQYLDGRREMMQWYADYIDSLSKQ
                     ESQG"
     gene            32764..>33653
                     /locus_tag="ACIE68_001907"
     CDS             32764..>33653
                     /locus_tag="ACIE68_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFX5908227.1"
                     /translation="MNDRLRFEVNDNQGRFVFPDTWFGPLLGEFEEVLDAYDTDEISE
                     TSYINKLRHLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRLIPESFSG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKKHADEFSPYWYELGLLHFLNGEQVKAATAFRRGFAANTYIAEML
                     CGNLHPFPLAVWHNYSAGPDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESI"
BASE COUNT         8398 a         8974 c         8373 g         7908 t
ORIGIN      
        1 cttttgctga gttgaaggat cagatcacgc atcctcccga caacacagac cattccgtgg
       61 caaagcaaaa gttcagaatc accaactggt ccacctacaa caaagctctc atcaaccgtg
      121 gctccctcac tttctggctg gatgatgagg ccattcaggc ctggtatgag tcggcaacgc
      181 cttcatcacg aggaaggccc cagcgctatt ctgatctcgc catcaccacc gttctggtga
      241 ttaaacgcgt attccggctg accctgcggg ctgcgcaggg ttttattgat tccatttttg
      301 ccctgatgaa cgttccgttg cgctgcccgg attacaccag tgtcagtaag cgggcaaagt
      361 cggttaatgt cagtttcaaa acgtccaccc ggggtgaaat cgcacacctg gtgattgatt
      421 ccaccgggct gaaggtcttt ggtgaaggcg aatggaaagt cagaaagcac ggcaaagagc
      481 gccgtcgtat ctggcgaaag ttgcatcttg ctgttgacag caacacacat gaagttgtct
      541 gtgcagacct gtcgctgaat aacgtcacgg actcagaagc cttcccgggc cttatccggc
      601 agactcacag aaaaatcagg gcagccgcgg cagacggggc ttacgatacc cggctctgtc
      661 acgatgaact gcgccgcaaa aaaatcagcg cgcttattcc tccccgaaaa ggagcaggtt
      721 actggcccgg tgagtacgca gaccgcaacc gtgccgttgc taatcagcgg ctgagcggaa
      781 gcaatgcacg gtggaaatgg acaacggaat ataaccgtcg ctcgatagcg gaaacggcaa
      841 tgtacagaat gaagcagttg ttgggagatt cactgacgct gcgtgactac gatggtcagg
      901 tagcggaagc tatggccatg gtgcgtgcgt tgaacaggat gacaaaggct gggatgccag
      961 aaagcgtgcg tattgcctga aaatccagcc agctacaggg tcgttcgcac gaaatcttat
     1021 ttattcaaca aagccgtgta tcacttcttt cgcggcaatc cgacccgaat gatttttaag
     1081 cacattgttg attttcaata aagaaatcaa tcaaataaaa tcattagcaa ccatattctt
     1141 tcactgttgc tctgcgattc cttcctgcac gtacagcaga aatcaatgaa tcgcataaaa
     1201 atacaaaaaa agtatagatg ttcagcaaga attggcgggt aaattgctat ttaagtcaaa
     1261 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
     1321 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
     1381 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
     1441 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
     1501 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgc catcgttatc
     1561 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
     1621 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
     1681 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
     1741 acctgattgc ggcgctacac aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
     1801 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgtcgc ttgctgcgtc
     1861 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
     1921 caaaaaataa tcaggtgacc gttaccggta gcctgaaatt cgatatttct gtaacgccgc
     1981 agttggctgc taaagccgtg acgctgcgcc gccagtgggc accacaccgc ccggtatgga
     2041 ttgccaccag cactcacgaa ggcgaagaga gtgtggtgat cgccgcacat caggcattgt
     2101 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
     2161 atgcgattaa tcttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
     2221 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     2281 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     2341 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     2401 aagacatttg cgcgcggctg gagcaggcaa gcgggctgat taccgttacc gatgccacta
     2461 cgcttgcaaa agaggtgtcc tctttactca ccgacgccga ttaccgtagt ttctatggcc
     2521 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     2581 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     2641 cctggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcaa
     2701 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     2761 ctggaagagc gtgtggcgct ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     2821 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     2881 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     2941 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     3001 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     3061 gagaatgtcc atcaggcgct gatggcgaag ttagcgtagc gtttatgccg gatggtatgc
     3121 catccggcgc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     3181 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcgccttt tcgcccgtaa
     3241 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     3301 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     3361 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     3421 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     3481 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     3541 tcgctgagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     3601 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     3661 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     3721 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag caaatattta
     3781 gcccgccgct gcacgctaag cactggttgg tcgcttaaac ggtagatctc ttctgaaacc
     3841 ggccagcgca agcgtccgtt gcgcaccact gcatgaagaa tggttgcacc aacgagatgc
     3901 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     3961 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     4021 ttttttgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     4081 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     4141 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     4201 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     4261 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     4321 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     4381 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     4441 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     4501 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     4561 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     4621 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     4681 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     4741 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     4801 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     4861 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     4921 agcgtgccgg aaaaaagtcg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     4981 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     5041 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg ccgcgccagt
     5101 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     5161 tgttcatatt cagaggttaa caagccataa agagagccga aattctccaa catctctttt
     5221 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     5281 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     5341 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     5401 aaatcgacgg ccagttttca atcctggaaa gcgcctcgca aagtgaatct caggtgaagg
     5461 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     5521 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     5581 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     5641 tgaccgaagc ggcaaaagcc tttatcaccc cacttagttt gcaggcggtt tctggttatc
     5701 ccgtttccga cagtctgctg gacccggcag ccgaagccgc tatgggccat attgagctgg
     5761 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     5821 ctgccggaat ggcaaatgat ctggtatcga cgatttgtct ggctacacct gcgcgtgtag
     5881 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     5941 aggtgcttgc ctcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     6001 gtgatatcgg ccctgggcga atgctcgatc cgttaaccat tgtggatatg gcggtagcgc
     6061 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     6121 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     6181 ctatcgctgc cgccgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     6241 cactaccgac accaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     6301 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     6361 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     6421 aattcacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     6481 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     6541 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     6601 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     6661 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     6721 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttgggaa ggaatttccg
     6781 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     6841 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     6901 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggcca taagcacggt
     6961 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     7021 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     7081 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     7141 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     7201 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     7261 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     7321 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     7381 tatcacgacg gcaaaactgg ctgcctctgt cggcgtttcc gaagcggcac tatatcgcca
     7441 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     7501 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     7561 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tccttaccgg
     7621 gcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     7681 tattgaagcg cagctgcgtc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     7741 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     7801 acgttttgtc cgcagcgagt ttaaataccg cccgacggat gattttgacg cccgctggcc
     7861 gctaattgcc gcgcagttgc agtaattctc ccctaaacgg tcaggcagaa acgtctgcct
     7921 gacgcttttc ttaccatgca accagcattc gctttacact ctctctcgcc tgttgaaagg
     7981 cctcatcctc agggttgatt tgcaataacc agtttattct tccctgcaaa gcataaagat
     8041 acgccgttgc ctgtgcgtgg agatcgccag aaggatcaag ttcaccacga tgactaagat
     8101 gcgaaataac gccatatttt tggcacaaat gcacctcctg acgcagcgcc cggcgcgctt
     8161 cacgggcaag ctgtggcttg tgattaacaa caatcccggt tacttgctgg cgcttacagg
     8221 cagctatcag gcagcctttg cgtttattga ggctcaggcc cagctccgct aacaatccgc
     8281 acactttatt tttaacctgg cgggcattga agtgacctga aaaggtcatg tcatcgcagt
     8341 agcgggtgta ggtaattccc cgagcctgac accattcccc tatgcgttca tcaaaacggc
     8401 gcatcacaag attggaaatg gctggcgaag ttggtgcccc ctgcggtaac gcgtcgttat
     8461 aacaacaaat ccaggtcagc atggttacca cattacgtgg caactgggcc tggcgaaaca
     8521 cacgccagac ctgtaaccag ctaatgctat cgaaaaagtt ttcgatatcg agtttcagga
     8581 tctgcggctg ttggcagtgt ggctgcgcgt tgctgacgat tgggcaacct ggtcggtagg
     8641 ctgtagcaaa aggggaaagc ggaaattgtg aaaggacgtt cttaagaatg ttgcgctgta
     8701 cggttttgag caagtaatca ggggctaaca ccagccgctg accaccgtgg cgtttagaaa
     8761 gaataatgcg tcggtaatgg cgtccggcat gattactgag cgcatgcagt cgctgtattt
     8821 ctttatcggc gctccagccc ggcgagccga acaaatcgag cgccagaagg gttgtccggg
     8881 tagcatccat tccactctcc acatcacggc ggccaaaaga ggcgccaaca gcggcaatac
     8941 cattaaacca tcccgaaggc gcgtaactgt actgagcgcg tcagcgcgac gtacgcgaag
     9001 cgtactcagg tacaatgaag cgactttggg tatatggaca tactactggc gttaagtcag
     9061 cgactcgccg accctcccgg cacaaaaggc cggaaacgct attgccactg ctggcgaaag
     9121 cagattaaca cgatatcttg aagcgcatca cgctgcgcac agcaaaaacc ctgcacagcg
     9181 catattttta aacgccgaac tcttcgcgat aggccttaac cgccgccaga tgttccgcca
     9241 tttccggctt ctcttccagg taagcaatca ggtctttcag ggtgatgata gagatcactt
     9301 tgcagttgta atcacgctca acttcctgaa tcgccgaaat ctcgccgcgc ccccgttcct
     9361 gacgatcgag cgaaatcaac acgccagcaa gcgtcgcgcc attggcctga ataatttcca
     9421 tcgactcacg aatcgccgtt ccggcggtga tcacatcatc taccagcatt acgcgtcctt
     9481 gtaacgcgct accaaccaga ttgccgcctt caccgtgatc ttttgcttct ttgcggttaa
     9541 agcagtacgg caggtccagg tcatgatgct ccgccagcgc cacggcggtt gtggtggcaa
     9601 tcgggatccc tttgtaagca gggccaaaca gcagatcgaa ctcaatgccg gaatccacca
     9661 acgcttcagc gtaaaaacgg cctaacagtg ccagatcgcg cccggtatta aacagcccgg
     9721 cgttgaagaa ataggggctt ttgcgcccgg acttcaacgt aaactcgcca aactttaaca
     9781 cctgcttgct aagcgcaaat tcaataaact ggcgctgata tggtttcatg ccttcgctcc
     9841 tcatcttact tttctacaga caaaaaaaag gcgactcatc agtcgcctta aaaatcagtt
     9901 tgccagcgcc gccttctgcg tcgctacaat ggattcgatt ccccctcggg ccagcgccaa
     9961 caaggtgagt aactcttcat gggtgaacgg ctcgccttct gccgtcccct gcacttcaat
    10021 aatgcgcccg tcttcggtca tcactacgtt catgtcggtc tctgcggctg agtcttcaac
    10081 gtattccaga tcgcaaattg cttcaccgtt tacaataccg acagaaaccg cggctaccat
    10141 ccctttcatt ggattggttt tcagcttgcc gttttccacc agcttctgta gcgcatctgc
    10201 cagcgccaca caggcacccg taatcgaagc ggtacgcgtg ccaccatcag cctgaagcac
    10261 gtcgcagtcc agcgtaatgg tgaactcacc cagcgctttc aaatctactg ccgcgcgaag
    10321 tgcacgggcg atcagacgct ggatttccat tgtgcgtcca ccctgcttac ctttcgctgc
    10381 ttcacgagcg ttacgggtgt gggtagaacg cggcagcata ccgtactctg cggtgatcca
    10441 gccctggccc tgacctttca ggaagcgcgg cacgccttct tcaatagagg cggtacacaa
    10501 cactttggta tcgccaaatt cgaccagcac cgagccttct gcatgttttg tatagttacg
    10561 agtcagggta acgggacgca cctgattatt gctacggcct gctggacgca tattgaaatc
    10621 tccggcttga aacaaatgtg gctgcgcatt atacggactt ccggcggtta ttcctatcct
    10681 gacaaggcat cgatggctat aatcctccca cctctccttt tataaacagg aacgtctatg
    10741 atccgcagta tgaccgccta cgcccggcgt gaaatcaagg gtgaatgggg gagcgcaacc
    10801 tgggaaatgc gctcggtaaa ccagcgttat ctggaaactt actttcgtct gccggagcag
    10861 ttccgtagcc ttgaacctgt cgttcgcgag cgtattcgtt ctcgcctgac gcgcggtaaa
    10921 gtggaatgta ccctgcgcta tgagccagat gttagcgcac aaggtgagct gatcctcaac
    10981 gaaaaactgg ctaaacagct ggtaactgcc gcgaactggg taaaaatgca gagtgacgaa
    11041 ggggaaatca acccggttga tattctacgc tggccgggcg tgatggcagc ccaggagcag
    11101 gatctcgacg cgattgccgc tgaaattctc gcggcgttgg atggtaccct ggacgacttt
    11161 atcgtcgcgc gcgaaaccga aggtcaggcg ctgaaagcat tgatcgagca gcgtctggaa
    11221 ggcgtcaccg ccgaagtggt caaagtccgc gcccatatgc cggaaatcct gcaatggcag
    11281 cgtgagcgtc tggtcgcgaa gctggaagat gctcaggttc agttggaaaa caaccgtctg
    11341 gagcaggagt tggttctgct ggcacaacga attgacgttg ccgaagaact ggaccgcctg
    11401 gaagcgcatg tcaaagagac ctacaacatt ctgaagaaaa aagaagccgt tggtcgtcgt
    11461 ctggacttta tgatgcagga attcaaccgc gagtcgaaca ctcttgcgtc gaaatctatc
    11521 aatgccgaag tgacaaactc cgccatcgag ctgaaagtgt tgattgagca gatgcgcgag
    11581 cagattcaga atattgagta actcgtaaca ctaattctta cttttcagcc ttcacaaatg
    11641 ccgcccactc aaacagagcg gcatttttct tccccgcaac ttcaattctg ttttgcgtgc
    11701 ctgctccaga ttttgcgatg tttttttgcc cagcacactg agaacgtgag atactcacaa
    11761 ctgtatataa atacagttac agatttactt tctttgcaat cgatatcaca tggagtgggc
    11821 aatgaacgaa catcatcaac cttttgaaga gataaaactg attaatgcaa acggagcaga
    11881 acaatggtca gcaagacaac ttgggaaact actgggttat tcagagtatc gtcactttat
    11941 acctgtatta acgcgcgcca aagaggcctg tgagaacagt ggtcacacaa ttgatgacca
    12001 tttcgaggag atcctcgata tggtcaaaat tggctcaaat gccaaaagag cattaaaaga
    12061 catcgtactc tcccgctatg cctgttacct ggtagtacaa aacggcgacc ccgcgaaacc
    12121 ggtcattgcg gcagggcaga cttattttgc tatccagacc cgacggcagg agcttgctga
    12181 cgatgaagca tttaagcaac ttcgtgaaga cgaaaagcgt ctgtttctga gaaatgagtt
    12241 gaaagaacat aacaaacaat tggttgaggc agcacagcaa gctaatacca cacactttga
    12301 cgtgggtagc aaggtgaggc aaaccattca ggaacttggc ggaaccatgc ctgaggagtt
    12361 gccgaccccg caggtcagca tcaagcagtt ggaaaacagt gtaaaaatta cagagaagaa
    12421 atagcagaaa cgaaaaactc tttttgttga ttgagacacc cgatgcgtaa gccaaggtcc
    12481 aggtgcagtg aacacaatgg ctaaacattg cacctttctt ttcccccctc agttttaacc
    12541 tattttttct tatgcatttt ctcagacaag aagtcagaag aatgcatcta cgctacagaa
    12601 aatagcgatt tcacataact acaagttatc aatttcctct caccttaaaa aatctcaatc
    12661 gtgacaatac gcacaaatcg ctaccctgcc agacagattt ttagggagag aaccatgctg
    12721 ttacacattt tgtatttggt tggcattact gccgaagcca tgacgggggc tctggcggcc
    12781 gggcgacgcc gcatggatac atttggcgta attattattg cgaccgccac cgcaattggc
    12841 ggagggtcag tgcgcgatat tctgctgggc cactatccgc tcggctgggt caaacacccg
    12901 gaatatgtga ttatcgtcgc taccgccgca gtactcacca ctatcgttgc tcctgtaatg
    12961 ccttacctgc gcaaagtgtt tctggtgctc gatgcgcttg gactggtggt cttttctatc
    13021 atcggcgcac aggttgcact ggatatgggg cacggcccaa ttattgccgt tgtcgcggcg
    13081 gtaaccaccg gcgtgtttgg cggcgttttg cgcgatatgt tctgtaaacg tatcccactg
    13141 gtattccaga aagagttgta tgccggtgtc tcatttgcct ccgccgtgct gtacatcgcg
    13201 ctgcaacact atgtttctaa ccatgatgtg gtaattatct ccaccctggt attcggcttt
    13261 tttgcccgct tactggcgct acgccttaag ctgggattac cggtctttta ctacagccac
    13321 gaaggacact aaggttcaaa acctgtgatc tgctgggcag ccagccaact gcccagcttc
    13381 ttgatttgcg cattttcctt ccattcaata acctgtctgg cgcgccccga tccagtcccc
    13441 ggcagttgct gccagaactg ctccgtgcta aataaaagtt gcgaccagga ccgttcatca
    13501 ctggcattaa gcgccgcccg ggttagcggt attcccattg ccatcaccca gcgagtaaaa
    13561 ggctgcttac gagccagatt aaactgatgc catagctgcg cacttttact tttcgcgatc
    13621 cccggcgtgt tctgtaattg ctctggcgtt aataaaagcc aggaaaagat atgctcaaag
    13681 cgatgagtct gatgcagcgc gcgccagccg gcctcaccaa tgccatccag ccccagaacc
    13741 tgttttgacc ccagccagac taagcgtgaa atgaactgtt cctgacaaac atcagaagca
    13801 aagtagcagg tcaacgagtt aaagcggttt tctggtggtg tcggttttgt acgttctgca
    13861 ccgcgccaga ccacatcatc aatgcgagga ataccctgac cggcaaggct gacgaggatc
    13921 tgatcaccag gcgcaatatc ccactcctgc cagcgcctga cggaaccaat attcacccgc
    13981 tggacttttt tatcatccag catgacaggt gcgagtgacg caaccaccga tattttaccg
    14041 ctcttaccca ccgcaaactg aatcgccttc acttcggcaa cctgagctac aggttgatat
    14101 ttccaggcca ccagccattc tgcctggcct ggtagccaat ggcgggattc gggttctttc
    14161 gcccctcgca caactacgcc atcggtgacg aagggtaatt ccgctttcca ccactcattg
    14221 cgtacgcgcg caacttcatc agcatttttc accgcacggg tatacgtctg cgttagagta
    14281 aaacctgcgg tagccagctc ttttaaacga tcagacatta actgcggtcc atccggccat
    14341 gcccagacaa aaacgcccag agaattcagc gtgtcgctat cgtcctggcg catcatcaag
    14401 ccagcaactt ttgcgcgggc atttattccc cccatttgtt gttggatatg cccctcgcgc
    14461 tggagaaata tttccccctg aagcgtactg ttggctaaag gcccgctaac ggtttgcggc
    14521 acagcggaaa ttaagctaac tttctgcgtc cagtcctcgc ctttcaggcc attaccgcga
    14581 ctgattgctt tgttcagttt cccgtcccga taaaccaggg ttaccgcaac gccatcaact
    14641 tttggctgca cccaaagatc gctacgttct cgcatccaca gacttaatgc gtttttatcc
    14701 accattttgc gtacacccgt atgggcaacc ggatgcataa ccgcaccatt taaaggcggc
    14761 atcatcacgt cgcggggctc gctcccaaag cagcgttgcc actgcgtaag acgggcgctt
    14821 aactgatcgt aaacaccgtc ttccacctca ctttttcctt ccttccagta gtcatcgtcc
    14881 cactgtttta tttgctgttg caggcgggaa atttcttcct gtgctctggc tggcgaccag
    14941 gccggacaga ccgcccacac agatgattgc cagcacaaga tacttattaa tatcgccatc
    15001 catactttca tcttcacctc cgctgtagtc aggctggcag atatacaacg tggtgaaggc
    15061 aaagccgagt ggcaaaaacg gagtctgcga ggacgcttcc tgagaatcgt ctttattgca
    15121 gtaaatcaca ggcaaatgcg gaaacagcta cgcaaaacgc aacaactttg cgcaaaaagt
    15181 gtgagcaagg gctacgtcac atggccgcgc cgtgtataat aagctcgtat gtaggcttta
    15241 tttcgctaat cacatacgaa agatactcat ggctcaaggc acgctttata ttgtttctgc
    15301 ccccagtggc gcgggtaaat ccagcctgat tcaggcttta ttaaaaaccc aaccgttgta
    15361 tgacacccag gtttctgttt cacacaccac acgccaaccg cgtcctggtg aagtccacgg
    15421 tgaacattat ttctttgtta atcatgatga atttaaagaa atgattagca gagatgcgtt
    15481 cctcgaacac gcagaagttt ttggtaatta ctatggcact tcgcgtgagg ccattgagca
    15541 agtactggcg accggtgtcg atgtttttct cgatatcgac tggcagggtg cgcagcaaat
    15601 tcgccagaag atgccgcacg cgcggagtat ctttatttta ccgccgtcca aaattgaact
    15661 ggaccgccgt ctacgcggtc gcggtcagga cagcgaagag gtcattgcaa agcgtatggc
    15721 gcaagctgtt gcagaaatga gccattacgc cgaatatgat tatctgattg tgaatgatga
    15781 cttcgatacc gcgttgaccg atttgaagac cattattcgc gccgaacgtc tgcgcatgag
    15841 ccgccaaaag cagcgtcatg acgctttaat cagcaaattg ttggcagact gaacctgatt
    15901 tcagtatcat gcccagtcat ttcttcacct gtggagcttt ttaagtatgg cacgcgtaac
    15961 tgttcaggac gctgtagaga aaattggtaa ccgttttgac ctggtactgg tcgccgcgcg
    16021 tcgcgctcgt cagatgcagg taggcggaaa ggatccgctg gtaccggaag aaaacgataa
    16081 aaccactgta atcgcgctgc gcgaaatcga agaaggtctg atcaacaacc agatcctcga
    16141 cgttcgcgaa cgccaggaac agcaagagca ggaagccgct gaattacaag ccgttaccgc
    16201 tattgctgaa ggtcgtcgtt aatcacaaag cgggtcgccc ttgtatctgt ttgaaagcct
    16261 gaatcaactg attcaaacct acctgccgga agaccaaatc aagcgtctgc ggcaggcgta
    16321 tctcgttgca cgtgatgctc acgaggggca aacacgttca agcggtgaac cctatatcac
    16381 gcacccggta gcggttgcct gcattctggc cgagatgaaa ctcgactatg aaacgctgat
    16441 ggcggcgctg ctgcatgacg tgattgaaga tactcccgcc acctaccagg atatggaaca
    16501 gctttttggt aaaagcgtcg ccgagctggt agagggggtg tcgaaacttg ataaactcaa
    16561 gttccgcgat aagaaagagg cgcaggccga aaactttcgc aagatgatta tggcgatggt
    16621 gcaggatatc cgcgtcatcc tcatcaaact tgccgaccgt acccacaaca tgcgcacgct
    16681 gggctcactt cgcccggaca aacgtcgccg catcgcccgt gaaactctcg aaatttatag
    16741 cccgctggcg caccgtttag gtatccacca cattaaaacc gaactcgaag agctgggttt
    16801 tgaggcgctg tatcccaatc gttaccgcgt aattaaagaa gtggtgaaag ccgcgcgcgg
    16861 caaccgtaaa gagatgatcc aaaaaatcct ctctgaaatc gaagggcgtt tgcaggaagc
    16921 gggaataccg tgccgcgtca gtggtcgcga aaagcatctt tattcgattt actgcaaaat
    16981 ggtgctcaaa gagcagcgtt ttcactcaat catggacatc tacgctttcc gcgtgatcgt
    17041 caatgattct gacacctgtt atcgcgtgct cgggcaaatg catagcctgt acaagccgcg
    17101 tccgggtcgc gtgaaagact acatcgccat tccaaaagcg aacggctatc agtctttgca
    17161 cacctcgatg atcggcccgc acggtgtgcc ggttgaggtc cagatccgta ccgaagatat
    17221 ggatcagatg gcggagatgg gtgttgccgc gcactgggct tataaagagc acggcgaaac
    17281 cagcaccacc gcgcaaatcc gcgcccagcg ctggatgcaa agcctgctgg agctgcaaca
    17341 gagcgccggt agttcgtttg aatttatcga gagcgttaaa tccgatctct tcccggatga
    17401 gatttacgtt ttcacaccgg aagggcgaat tgtcgagctg cctgccggtg caacgcccgt
    17461 cgacttcgct tatgcagtgc ataccgatat cggtcatgcc tgcgtgggcg cacgcgttga
    17521 ccgccagcct tacccgctgt cgcagccgct taccagcggt caaaccgttg aaatcattac
    17581 cgcaccgggc gctcgcccga atgccgcgtg gctgaacttt gtcgttagct caaaagcgcg
    17641 cgccaaaatt cgtcagttgc tgaaaaacct caagcgtgat gattctgtaa gcctgggccg
    17701 tcgtctgctc aaccatgctt tgggtggtag ccgtaagctg aatgaaatcc cgcaggaaaa
    17761 tattcagcgc gagctggatc gcatgaagct ggcaacgctt gacgatctgc tggcagaaat
    17821 cggacttggt aacgcaatga gcgtggtggt cgcgaaaaat ctgcaacatg gggacgcctc
    17881 cattccaccg gcaacccaaa gccacggaca tctgcccatt aaaggtgccg atggcgtgct
    17941 gatcaccttt gcgaaatgct gccgccctat tcctggcgac ccgattatcg cccacgtcag
    18001 ccccggtaaa ggtctggtga tccaccatga atcctgccgt aatatccgtg gctaccagaa
    18061 agagccagag aagtttatgg ctgtggaatg ggataaagag acggcgcagg agttcatcac
    18121 cgaaatcaag gtggagatgt tcaatcatca gggcgcgctg gcaaacctga cggcagcaat
    18181 taacaccacg acttcgaata ttcaaagttt gaatacggaa gagaaagatg gtcgcgtcta
    18241 cagcgccttt attcgtctga ccgcccgtga ccgtgtgcat ctggcgaata tcatgcgcaa
    18301 aatccgcgtg atgccagacg tgattaaagt cacccgaaac cgaaattaat gttttatgaa
    18361 cccaacacgt tatgcacgca tctgcgaaat gctcgccagg cggcagcctg atctgaccgt
    18421 ctgcatggag caggtccaca aacctcataa cgtttctgcg attattcgta ccgcagatgc
    18481 cgttggcgta catgaagttc acgccgtctg gcctggtagc cgcatgcgca ccatggcttc
    18541 ggcagcggcg ggtagtaaca gctgggtaca ggtgaaaaca caccgcacca ttggcgatgc
    18601 cgtcgctcat ctcaaaggcc agggcatgca gattctggca acccatcttt ctgataacgc
    18661 tgtcgatttc cgcgaaattg attacactcg cccgacctgc attttgatgg gacaggagaa
    18721 aacgggcatc acgcaggaag cattggccct ggcggatcag gacatcatca ttccgatgat
    18781 cggcatggtg cagtcgctga atgtttccgt tgcctcagcc ctcattcttt acgaagccca
    18841 gcgtcagcgg caaaatgcag gcatgtacct gcgtgaaaac agcatgttgc cggaagcaga
    18901 gcaacaacgc ctgttgtttg aaggcggcta tccggtgctg gcgaaagtcg caaaacgcaa
    18961 aggcctgcct tatccccacg tcaatcagca aggcgagatc gaagctgatg ccgactggtg
    19021 ggctactatg caggctgcag ggtaagtgcc atgaaaggtc gcctgttaga tgctgtccca
    19081 ctcagttccc taacgggcgt tggcgcagca cttagtaaca aactggcgaa aatcaacctg
    19141 cataccgtgc aggatctact cttacacctt cccctgcgct acgaagatcg cacccatctc
    19201 taccccatcg gagaactact gccgggcgtt tatgccacgg tggaaggcga agtgctgaac
    19261 tgcaatatct ccttcggcgg tcggcggatg atgacctgcc agatcagcga cggttccggc
    19321 atcctcacca tgcgcttttt caatttcagc gcggcaatga aaaatagcct ggcgacgggc
    19381 cgccgtgtac tggcttatgg cgaagcaaag cgcggtaaat atggtgcgga gatgatccac
    19441 ccggaatacc gcgtgcaggg cgatctcagc acgccagaat tacaggaaac gctcacgccg
    19501 gtttatccaa caacggaagg cgtaaagcag gccacgctgc gtaaattaac cgaccaggcg
    19561 ctggatctgc tcgacacctg cgccattgaa gaactcctgc cgccggaact gtcacaagga
    19621 atgatgacgc taccggaagc gttgcgcact ttgcaccgcc cgccgccgac gctacagctt
    19681 agcgatctgg aaaccgggca gcatccggcg caacgtcgtc tgattctgga agaactgctg
    19741 gcgcacaacc tcagcatgtt agccttacgt gccggagcac agcgttttca tgcccagccg
    19801 ctgagcgcca atgacgcgct gaaaaataaa ctcctcgccg ccttaccgtt caagccaacg
    19861 ggcgcacagg cacgcgtagt ggcggagatc gagcatgata tggcgctgga tgttccaatg
    19921 atgcgcctgg tgcagggcga tgtaggttcc ggtaaaacac tggtcgccgc cctcgccgcg
    19981 ctgcgtgcga ttgcccacgg caaacaggtg gccctgatgg caccaaccga attactcgcc
    20041 gagcagcacg ccaataactt ccgcaactgg tttgcaccgc tcggtatcga agtgggctgg
    20101 ctcgccggta agcagaaagg taaagcgcga ctggcacagc aggaagccat cgccagcggt
    20161 caggtgcaga tgattgtcgg tacacacgcc atcttccagg aacaggtgca gtttaacggt
    20221 ctggcgctgg tgattatcga cgaacagcat cgttttggcg tgcatcagcg tctggcattg
    20281 tgggagaaag gccaacagca gggcttccat ccgcatcagt tgatcatgac cgccacgccg
    20341 atcccccgca cgctggcaat gacggcgtat gccgatctcg atacctcggt gatagatgag
    20401 ctgccgccag gccgcacgcc agtgactacg gtcgctattc ctgatacccg ccgtaccgac
    20461 atcattgacc gcgtgcgcca cgcctgcatg actgaaggtc gtcaggcgta ctgggtttgt
    20521 acgttgattg aagagtcaga gttactggaa gcgcaggcgg cggaagctac ctgggaagag
    20581 ttgaaactgg cgctaccaga attgaatgtt ggcctggtac acgggcggat gaaaccagcc
    20641 gagaaacagg cggtgatggc gtcgtttaaa caaggtgagc tacaactgct ggttgccact
    20701 accgttattg aagtcggcgt tgatgtgcct aacgccagtc tgatgattat cgaaaaccct
    20761 gagcgtctgg gtctagcgca gttacaccag ctgcgcgggc gcgtaggtcg tggcgcggtg
    20821 gcttctcact gcgtgctgct ctacaaaacg ccgctctcca aaacggcgca aattcgcctg
    20881 caagtgctgc gcgacagtaa cgacggtttt gtgattgcgc aaaaagatct ggagattcgc
    20941 ggccctggcg aattgttagg cacgcgtcag acgggtaatg ctgaatttaa agtggcggat
    21001 ttactgcgcg atcaggcgat gatcccggaa gttcagcgcc tggcgcgaca tattcacgaa
    21061 cgttacccac aacaagcaaa agccctgata gaacgctgga tgccggagac ggaacgttac
    21121 tccaacgcat gaccagactc atcacaagac aagtcacgca gtaaatcttc aatctggata
    21181 gtgaggatat ccattgcctc atacgcatcc tgtgcaattc gcgccgccat tgcgccttcc
    21241 ggttcaccac gcagaatttc agccgctgcc tgtacgccag ccacaatcat ttcaggtgtt
    21301 aacatttggc gaagaaaagc gcgattatca gaggaccagc catctttatg gcctaatgga
    21361 tgcccccagc ctatccattc ttgcgaccat aacggattgg cttctatggg ataaattaga
    21421 tgcgcggcat actgaatagc acacaccgct tttcctggcg tatccagcgc ccaccagcgc
    21481 gaccagagag aacgaattaa cggcgcggcc ccacccagag tattgaacac cgccagccag
    21541 caaagaacgt tattgaaacc acgctcgtta tccatacgct gcaacgcggt gtccagtaaa
    21601 aattgccgta cctgctggcg ttgggaagca ttcatcatct cctgccagag atagggccga
    21661 cacaatgcct tcattaagga ttcttcgaag tgatcaccat aaccgccagc taaaacggtt
    21721 tcatgccatt gcgccaggca gagcggaaac agatagcgaa acaactctat ttgcatcggt
    21781 tcgttataaa cgagattgag tacatagtaa gcactcaaat cccattcgct gatctgctgc
    21841 cactctttag ccgccagcgc cttgagttcg gcatctgcgt aatcaaattc cgtttcccat
    21901 acacagagca ctggcggctc atcaccaaaa atatggcgac agtgttcacg tagcgtttcc
    21961 ggttccttcc gacgcttttt catttttaac cggcaaaaat cggcaacatt aaatacagct
    22021 taatcaccag cgcattgacg atatcgataa agaacgcacc gaccatcggc accaccaaaa
    22081 acgccatgtg cgacgggcca aagcgttcag tgatcgcctg catgttggcg attgccgttg
    22141 gcgttgcacc gaggccaaaa ccacagtgac ccgcagccag cactgccgca tcgtagtttt
    22201 tgcccatcat gcgccaggta acgaagatgg catacaacgc catgaagatg gtctgtacca
    22261 ccagaatcgc cagcatcggc agcgccagcg aagccagctc ccacagtttc agccccatca
    22321 gcgccatcgc caggaacaac gacaggctta cgttacccag cacggatacc gcacgctcaa
    22381 agacgcggta aaagcccatc attgacagac cgttgctcag aatcacgcca acaaacagga
    22441 cacagacgaa ggtcggcagt tcaaaagcag tgccagccaa aagttgcgca acaattttcc
    22501 ccaccgtcag gcagatagca atcagcgcga tagtttcaat cagcaccagc gaggtgatca
    22561 tgcggcccac atccggcttt tcaaacgccg tcgggacttc ctggtcatcc ggaataccgt
    22621 tcggcgtggt ggagtgtttc accagatagc gcgccaccgg accgccaatc aagccgccca
    22681 gcaccagacc gaacgttgca caggccatcg ccacttccgt cgcattggtg aagccataac
    22741 gttcaatgaa caatttactc cacgcagcgc ccgtaccgtg accgccggaa agcgtaatag
    22801 aaccggccaa cagccccatc agcggatcaa gccctaacag gctagccata ccaatgccaa
    22861 tggcattttg catcaccaac agaccaacaa ccacaatcaa gaagatgcca accacacgcc
    22921 caccggcacg caaactggca atgttggcgt tcaggccaat ggtggcgaag aaagccagca
    22981 ttaacggatc gcgcagggac atatcaaagt tgacttccca gcccatgctt tttttcagta
    23041 ctagtagcgc cagcgccacc aacaaaccac ccgcaacagg ttccggtatg gtgtatttct
    23101 tcaaaaagga gacggaatgg accaacttac gcccgagcag caacgtcagc gttgcggcaa
    23161 caagcgttgc taaagtatcg agatgaaaca tagttactcc tttgtatccg catcactctt
    23221 tcatactcgt catacttcat cgcgcagtgg caacttcatg aactatgacg ggtatcgagt
    23281 tatgctttcc tgggtcaaat tctcagctgt taatgaacaa atggcatgga ttttaagcag
    23341 aaaaatacca aaagttatat aaaaaagtgc atttatctca tttaaatatt tagatgagga
    23401 tgtggcaaac gtttgctttt ctctttcggt cagctaaaat gcccgctttg ctaccacggg
    23461 attgttttcg atgtctgttt ccaccctcga gtcagaaaat gcgcaaccgg ttgcgcagac
    23521 tcaaaacagc gaactgattt accgtcttga agatcgtccg ccgcttcctc aaaccctgtt
    23581 tgccgcctgt cagcatctgc tggcgatgtt cgttgcggtg atcacgccag cgctattaat
    23641 ctgccaggcg ctgggtttac cggcacaaga cacgcaacac attattagta tgtcgctgtt
    23701 tgcctccggt gtggcatcga ttattcaaat taaggcctgg ggtccggttg gctccgggct
    23761 gttgtctatt cagggcacca gcttcaactt tgttgccccg ctgattatgg gcggtaccgc
    23821 gctgaaaacc ggtggtgctg atgttcctac catgatggcg gctttgttcg gcacgttgat
    23881 gctggcaagt tgcaccgaga tggtgctctc ccgcgttctg catctggcgc gccgcattat
    23941 tacgccgctg gtttctggcg ttgtggtgat gattatcggc ctgtcgctaa ttcaggttgg
    24001 gttaacgtcc attggcggcg gttacgcagc catgagcgat aacaccttcg gcgcaccgaa
    24061 aaatctgctg ctggcaggcg tggtcttagc cttaattatc ctgcttaacc gtcaacgtaa
    24121 cccttactta cgcgtggcct cactggtgat tgcgatggcg gccggatatg cgctggcgtg
    24181 gtttatgggc atgttgccag aaagcaacga accgatgacg caagaactga ttatggtgcc
    24241 aacgccgctc tattacggtc ttggcattga atggagtctg ctgctgccgc tgatgctggt
    24301 ctttatgatc acttcgctgg aaaccattgg cgatatcacg gcgacctctg acgtttccga
    24361 acagccagtg tccggtccgc tgtacatgaa acgcctgaaa ggcggcgtgc tggcaaacgg
    24421 cctgaactcg tttgtttcgg cggtgtttaa caccttcccg aactcctgct tcgggcaaaa
    24481 caacggagtg atccagttga ctggtgttgc cagccgctat gtcggttttg tcgtcgcgct
    24541 gatgttgatc gtgctgggtc tgttcccggc agtgagcggt tttgtacaac acattccaga
    24601 accggttctg ggcggcgcaa cgcttgtaat gtttggcacc atcgccgcct ccggtgtgcg
    24661 tatcgtttct cgtgagccgc tgaaccgtcg ggcgattctg attatcgcgc tgtcgctggc
    24721 ggttggtctg ggcgtgtctc agcagccgct gattttgcag tttgcccctg aatggctgaa
    24781 aaacctgctc tcctccggga tcgccgcggg cggtattact gccatcgtgc tgaatctgat
    24841 tttcccacca gaaaaacagt aattcactgg cggcggtaag ataattaccg ccgcagtctc
    24901 acctctttca ccattcccgc cttgaggatt gcgcgtaaat cgtgcataac tcccttatgt
    24961 gcatttcacg ggatggaaga ccatgaaatt tattgggaag ctgcttctct acattctcat
    25021 cgctctgtta gtggtgatcg ctggcctcta ttttcttctg caaacccgct ggggagcaga
    25081 acatatcagc gcatgggttt ccgagaatag cgactatcat ctggccttcg gggcgatgga
    25141 tcaccgtttt tccgcgccat ctcatatcgt gctggagaac gtcacgtttg gtcgtgatgg
    25201 tcagcccgcg accctggtgg caaaaagtgt cgacattgcg ctaagcagtc ggcaactgac
    25261 cgaaccacgc catgtcgata ccatcctgct ggaaaacggg acgctgaatc tcaccgacca
    25321 gaccgcgccg ctaccgttca aagccgatcg tctgcaactg cgtgatatgg cgtttaatag
    25381 cccgaatagc gaatggaaac tgagcgcgca gcgggtaaat ggcggcgtgg ttccgtggtc
    25441 accagaagcc ggtaaagtgc tgggtacgaa ggcgcagatt cagtttagtg ccggatcgct
    25501 ttcgctcaat gatgttcctg ccaccaatgt actgattgaa ggcagtattg ataatgatcg
    25561 cgttacgctg actaacctgg gtgccgacat cgcccgcggg acattaaccg gaaacgcgca
    25621 gcgtaacgcc gacggcagct ggcaagtgga aaacctgcgc atggcggata tacgtctaca
    25681 aagcgaaaaa tcgctaaccg acttctttgc gccattacgc tctgtcccgt cgttgcagat
    25741 tggtcgcctg gaagtgatcg atgctcgttt gcaaggtccg gactgggcgg tgaccgacct
    25801 cgatctcagc ttgcgcaaca tgaccttcag taaagatgac tggcagacac aagaaggcaa
    25861 actgtcgatg aacgctagcg agttcattta tggttcgctg catttatttg acccgattat
    25921 aaacgcggaa ttttccccgc agggcgtagc gctgcgccag ttcaccagcc gctgggaagg
    25981 gggtatggtc agaacgtcag ggaactggct gcgtgacggg aaaacgttga tccttgatga
    26041 tgcggcaatt gccgggctgg aatatacctt gccgaaaaac tggcaacagt tgtggatgga
    26101 aacgacaccc ggttggttaa acagcctgca actgaagaga tttagcgcca gccgcaatct
    26161 gatcattgat atcgaccctg acttcccgtg gcagctcacc acgctcgatg gttacggtgc
    26221 caacctgacg ctggttaccg atcataaatg gggcgtctgg agtggctcgg cgaatctgaa
    26281 tgccgccgcc gcgacattca atcgtgttga tgttcgtcgc ccgtcgctgg cgctgaccgc
    26341 caacagcagc acggtgaata tcagcgaact gagtgcattt actgaaaaag gcattctgga
    26401 agccaccgcc agtgtttcac aaacgccaca acgtcagaca catatcagcc tgaatggacg
    26461 cggtgtgccg gtgaatattt tgcaacagtg gggatggcct aaattaccgt tgactggcga
    26521 cggcaatatt cagcttaccg ccagtggcga tattcaggcc aatgtcccgt tgaaacctac
    26581 ggttagcggg caattgcatg ccgtgaacgc cgcaaagcag caagtgactc aaaccatgaa
    26641 tgctggcatc gtttccagcg gtgaagttac atcgacggag ccggtgcggt aaacatcaat
    26701 ctcgccgggt acgactcaga cgtacccggc attccatcaa tagattacaa cgtaattgtc
    26761 agcgcattcc cttgaggctt caccaccagc ccctgctcac tttcagcctg cgaaccgtct
    26821 tgcagaccat ttactttcac aacattgcgc aggcacagcg tccagttctt cgcctcgccc
    26881 gcaccagtca cagtaatcgt gttgccagta cgtgctgctt ttaaagtaaa gatcaccgat
    26941 ccgtcagtag cgggcacttc acagacggct tcatgcccgt cttgcagatt aaagaggtgg
    27001 aatgccgtgc cttcgtgcca cacgtaatcg ggacgttgat cgttgttgcc cagcgccagt
    27061 agagtgttat cacgcacata aacgggcaga ctcaggaagc cgtgctgctg tttatgccag
    27121 cgactaccgt cgagttcatc gttgtgccac aggtgtgtcc agcgaccttc cggcaggtag
    27181 aactgcacat cgcccgcttc agtgaacacc ggcgcaacca tcacgttgtc gcctaacatg
    27241 tattgacggt caaggtaatc acaagccgga tcgtccggga actccatcat catggcccgc
    27301 atcatcggcg taccccgcgc gttcgcacgc gcagcttcac gatacagata cggcatcatg
    27361 cggcatttca gttgcgtgaa gaagcgcacc acatcacagg actcatcatc gtaggcccac
    27421 ggcacacgat aagatttgct accgtgtaaa cggctatggc tggagagcaa accaaacgcg
    27481 caccagcgtt tgtaaacgtg cgccggagcg gtattttcaa agccgccgat atcgtggctc
    27541 cagaagccaa aacctgaaag gccaatagac aaaccaccgc gcaggctttc cgccattgat
    27601 tcgtagttag cgtaacaatc gccaccccag tgtaccggga atttctgcgc accgacggag
    27661 gccgagcggg caaacaagac agcttcttcc tcaccaacgg tgtccttgag cacgttccac
    27721 accagttcgt tgtagatgta cgcataatgg ttatgcattt tctgcggatc ggaaccgtca
    27781 aaccactgaa catcagttgg gatacgttcg ccaaagtcgg tcttaaagca atcaacgccc
    27841 atcgcgacca gacctttcag tttgtcggcg taccatttgc aggcatccgg attggtaaag
    27901 tcataaatcg ccagacctgg ctgccattta tcccactgcc atagcgaacc gtcggggcgt
    27961 ttgagtaaat agcctttctc ttgtaactct ttaaagacgg gggatttttg accgatatag
    28021 gggttaatcc agacgcagat tttcagccct ttcgctttca ggcggcggat catcccttcc
    28081 gggtcaggga aagtcagcgg gtcccactca aaatcgcacc actggaaggc tttcatccag
    28141 aaacagtcaa agtggaaaac atgcagcggc agattgcgtt ccgccatacc atcgataaag
    28201 ctgtttaccg tcgcttcgtc gtagttggtg gtaaatgaag tggttagcca caggccgaag
    28261 gaccacgcgg gcggcagcgc cggacgacca gtaaagcggg tataacgatc aagtaccgct
    28321 ttcggcgtcg ggccgtcgat aacaaagtat tcgagatatt cactctcaac gctgaactgc
    28381 actttggaga ctttctccga tcccacttca aaagagacac actggggatg attgaccagt
    28441 accccataac cacggttagt catgtagaac gggatatttt tatacgcctg ttcagtactt
    28501 gtgccgccgt cccggttcca ggtctctacc gtctggccat tgcgcaccag ggcagtaaag
    28561 cgctctccca gaccgtaaac tgtttcgcca acgccaagat caagccgctc aaacatataa
    28621 ttgcgttgat tattcgtgtc ctgcacgtag ccattatttt tcacctgact accggtaata
    28681 cgttcgccgt tgcgcagaaa atccagtgac cagaactcac ctttgctgac acgcgcgctt
    28741 aagttgccac ttttaaactc agcataacgt tctgtgtttt cgattgtgac cttcacgtcc
    28801 tgcaaaatat tgagcggata atgaggaccg ttattcagcg ccccctgaaa atgctcaatc
    28861 cgcacaccga caataccttc ctgtggggag aaaaagcgca acgtaaataa aggcgtatca
    28921 agctgccagg tacgttcacg cacatcacgg ggggcagcat agaccaccat ttcattatcc
    28981 tgctgttcaa cctcgaacac ctgaagcggg tgaatcaaat tgaggccagg ttgaatcaac
    29041 cagtttccat cgctaatttt cattgccgtt ccttagttct gcacttcttg agaggtgaat
    29101 tgttgctggc aacggcgttt accctgagcc agctgttcca taacggtttt cagattgtgc
    29161 gtcgtgagtg agtagtagcg tttagcgata atcgcgctca gcaaataaca gatcgccgga
    29221 acaatcgtga atagcgcaat aatgatgcta atcgtggcgc tgttctgcgc tttttctgcc
    29281 gcatcatatc cgccataagc cagcatccag ccgataagag cgccgccgaa ggccaacccc
    29341 agtttgagca caaacagcgt gccagcaaaa ctgatcccgg tcaggcgttt accattgcac
    29401 cactcgccgt agtcgacggt atcggacatc attacccact ggataggtgt caccagttga
    29461 tgcaacacac caatcacgaa gatgaagaca aacatagtga tgctggcctg catgggaaca
    29521 aagaacatcg cgaggctaat cactgccagc agggcgttcg tccaccagaa gatagtgact
    29581 ttacatttcc agtcggtcag aggttttgcc agtgcggaac caatcaggtt acccacgcaa
    29641 taagtggtga gaaaagcgac aaacacttcc ggcgtgccca aaatccatgt gacgtaatac
    29701 atcatcgccc caccgcgtac gcacaccgcc aggatattga aaatggttag taaaccgaca
    29761 atccgccact ggtcgttttg ccagatatca cgtaaatctt cccgcataga cgttgttgta
    29821 ggtggtgctt caacgcgttc tttagtggtg aagaaacaaa atgccagcat catgaatgcc
    29881 accacggaaa ggaccgcgat accgccctgg aaaccgagtg gtttattatc accgccaatt
    29941 aaattaacca gtggcatcat cagaacagta gaaagcatgc ctcccgcggt cgccagcaca
    30001 aaacgccagg attgcagcga gatacgctga gtcgggtcat tggtgattac accacccaat
    30061 gcgcagtaag ggatattgac gacggtataa agtaaggtaa gtagggtgta agtaattgct
    30121 gcatagatca ttttgccgtt catactgaga tctggcgtgc tataggccag tacacagacg
    30181 atcccgaatg gcagtgcgcc aaacagtacc cacggacgaa atttacccca gcgagagcgc
    30241 gttcgatcgg ccaacaaccc catgcaagga tcggaaatcg catccagtgc acgagcgacc
    30301 aaaaacatgg ttccgacaaa accggcagga atgccaaaaa tatcggtata aaagaacatc
    30361 atatataaca ttacgttatc gaaaataatg tggctggcgg cgtctcccat gccataacca
    30421 attttctctt taacggacaa cacttcactc ttcatctgct tttccttgat gctatagggg
    30481 tgctgagacc gataacgcat tttgtaaacc atccctgtga gagcgaatat tccgatatct
    30541 ggttattaat ttatgattct tgttttatgt gatcgtggta gcgttaattc cgctcatata
    30601 tcattgtaaa atatgggttt tatatgaact ataatgcttt cgtgataata cgctgcgtgt
    30661 attaggcgga aaaaactgat ctgggggatg tagaaactca aggaagtagc tataatgcgc
    30721 cccgcctcca tgtagcaatc gaggcgcgga agatcgtcgt ctccggtgag gcggctggac
    30781 ttcaaatcca gttggggccg ccagcggtcc cgggcaggtt cgactcctgt gatcttccgc
    30841 caattaacat cttctgaact ctcccgatgt caaccccccc ttctaatccc ttgtatatca
    30901 ggattccttt gtatccagaa gtcaaccgac ttcaccccgt atcaagttgc ttttgggggt
    30961 actttagggg gtattatttc ctattgaaat atcagcaccc ccataattgg taaaatatga
    31021 tatataacaa cggctttcaa caacatcttt gttatacaag gggtgcattg ggggtatcag
    31081 gttaattact ggcagaagtc cccctcagga acatgaattc attaaaagcc ggaggatgtc
    31141 atggcattaa ccgatattaa agtgaaaacc gcaaaaccaa aagaaaagcc ctataaactt
    31201 gctgatggcg gcggtatgta tctactgatt aacactaatg gttctaaata ctggcgtatg
    31261 aagtaccgct ttgctggtaa agaaaagatg ctttctattg gcgtataccc cgatgtaacg
    31321 ctagccgacg ctcgtgaaaa acgtagcgaa gccagaaaaa tcctcgctgc tggaggcgat
    31381 ccaggggtgg taaaaaaaga agaaaaaatc gcgcaacaaa tgagtttgaa aaatacgttc
    31441 gaggccattg ctcgcgaatg gcatcagtca aaagccgatc ggtggtcatt acgctatcgt
    31501 gatgaaatca ttgatacctt tgaaaaagac atttttccct acattggcaa acgccctatc
    31561 gccgaaatca aaccaatgga gttactggaa gcactacgta aaatggaaaa acgtggagct
    31621 ctggagaaaa tgcgcaaagt ccgccagcgc tgtggtgaag tgttccgcta tgcaattgtt
    31681 actggccggg cagactacaa ccctgctccc gatcttgcct gtgctttagc tacaccgaaa
    31741 aaagtacatt taccctttct tactgctaat gaacttcccc acttcctcaa cgatctggcg
    31801 ggttataccg gaagtatcat cactaagaca gctactcaga tcattatgtt aactggtgta
    31861 cgaacacaag aattacgttt cgcacgctgg gaggatatcg attttgagac aaaattatgg
    31921 gagattccgg cagaagtaat gaagatgaag cgacctcata ttgtgccgct ctctgagcag
    31981 gtcattgccc tatttaagca acttgaaccc atcacaaaac atcatcctct ggtctttatc
    32041 ggtaggaacg atcctcgcaa gccaattagt aaggaaagca ttaaccaagt cattgaatta
    32101 ctgggatata agggaagact cacagggcac ggtttcagac acaccatgag taccattctg
    32161 cacgaacagg gctttaattc tgcctggatt gaaatgcagt tagctcatgt ggataaaaac
    32221 gccatcaggg gtacctataa tcatgcccag tatctcgatg gtcgccgtga aatgatgcag
    32281 tggtacgcag attatattga ttcgctttct aagcaagaga gtcagggtta agagcacttg
    32341 tcctgatatt ttctctcaat ccacaaattc tcctccggcc tccaggtccg gaggaatttt
    32401 gtttttatgc tttattttta gatatttttt gttttttgac aaaatattta atattgcatc
    32461 tgtaaacaat ctcaaaacat cacaccgtta tggaccgcta acaattttga tacctggagt
    32521 ttcaatcatc atcctgtcgt tatttcagcg ccatcacaaa aaaaatcgat taatatgctg
    32581 tttcagctga caactcacaa tacaccggaa tgcagataaa gataaatttt cattctcatg
    32641 taaaatcact gtcgatgata ttgtaaacaa cattcatccc ggacaaaaac aggacttgat
    32701 acacaacatc acgactttta tgcttttcag attatgtccc catccgtttt acaggctcag
    32761 aaaatgaacg acagattacg ctttgaagtg aacgataacc aggggcgttt cgtatttccg
    32821 gatacctggt ttggcccatt actgggggag tttgaagaag tgcttgatgc atacgacact
    32881 gatgaaatca gcgagacgag ttacatcaac aaactcagac accttgctca acgggagcct
    32941 gactttattg atatacacgc tcatctggca tatgcatttc tggagcagaa tgccccgcgt
    33001 aaggctctga acgccgcgct taaaggactg gcggccggta atcgtctcat cccggaaagt
    33061 ttcagtggtg aaatcatctg gatgcatccc gaaaaccggc cttatctgcg cgcgctttat
    33121 gcagcaattc tcgcaaatgt gcacttacag cgtcatcagg atgcggttat gcttactgat
    33181 aaaatactgg catacaatcc tgaagataat cagggtgccc gctggttgtt gggttcagaa
    33241 ctgttacgaa caggtgatca tgagcgagcc ttcagtgtac tgaaaaaaca tgctgatgaa
    33301 ttttcacctt actggtatga acttggtctt ctgcattttc ttaatgggga acaggtaaaa
    33361 gctgcaactg catttcgtcg cggatttgcg gcaaatactt acatcgcgga aatgctgtgc
    33421 ggaaatctgc atccatttcc tcttgctgta tggcataact attctgccgg gccggatact
    33481 gccgaagatt attatgcaac atactcacca ttatggggac aatatccgga agcattgctg
    33541 tttgtaaact ggctctataa ccattcctct gtgctgcatg aacgggcaga aatcattaaa
    33601 tgcgccgaaa tgctgatgca ggaagatgat ttcgaaatat gcgaaagtat ttt
//
LOCUS       DBANUU010000042        33359 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000042 DBANUU010000000
VERSION     DBANUU010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.042"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..146
                     /locus_tag="ACIE68_001908"
     CDS             <1..146
                     /locus_tag="ACIE68_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFX5908228.1"
                     /translation="GYITRRYQCFPLAPLPEDRKYDSLPDLVLHPEQETIKCKEKHYS
                     QPL"
     gene            113..1315
                     /gene="traW"
                     /locus_tag="ACIE68_001909"
     CDS             113..1315
                     /gene="traW"
                     /locus_tag="ACIE68_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HFX5908229.1"
                     /translation="MQRKTLLAALIATLSGTACQAHAYSVTVVASRPVEEQVIPRMEA
                     IKDVLGNILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            1344..1928
                     /gene="traX"
                     /locus_tag="ACIE68_001910"
     CDS             1344..1928
                     /gene="traX"
                     /locus_tag="ACIE68_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HFX5908230.1"
                     /translation="MNKLPENSDQTKNVKKSLSITRIATRGIINIFVPLMETRLITNS
                     VQHMLEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRFCC
                     WSLLAIALFLSGMLFAASSLPLTTLVRAISTLVLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
     gene            2025..4193
                     /locus_tag="ACIE68_001911"
     CDS             2025..4193
                     /locus_tag="ACIE68_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFX5908231.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSAPTLIGSMFGAFNSIIAVLAVVWFMFIGIRHVVRSGHQGQVFST
                     GRDIVGTLSVVAGFLMIVPTGNGWSIAQLIMLWGASIMGVGSANVMVQLAADNIANGY
                     SMTVQPVQASTRTAARGIFEMELCKYAVNAGLNDFNQTVKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGSASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGTLPDIETRIQRAADEYERAVQKSLPIDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMGAYK
                     TQLQNSTYTPTIGTITTQDESNAANSTDPQSVVMKSIAPTILKWTNQAATEWSGTGTT
                     SNQVNPLIKMKNIGDYTLGTTEILWTGYTTVRVLATMGDNSIFGKAVNLFSGLPKGFV
                     ALLDAAAPPIYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWA
                     ATHLGTSEDRGSRAAYGYIYLIDSMIRPPLMVFGFFFASVAIIAVGTILNSLFGAALV
                     NVQVKSLTGIFSLAGFLLIYARMCTTSVAAIFALQAYLPDHVINFLGGRDGVNTLGNL
                     TSSVKDIFAGSNRNIRHSPGVREDRLKNVTKDDNKDGIKG"
     gene            4267..4917
                     /gene="excA"
                     /locus_tag="ACIE68_001912"
     CDS             4267..4917
                     /gene="excA"
                     /locus_tag="ACIE68_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFX5908232.1"
                     /translation="MQMVKTINRYPAWWTLYYALRFIYFISIPVLSIFILFGVLSITS
                     SRYVTQEDYIYTCVCLFLLIAPAILMYSRASSRKDKIKKIVAEIKNTGFYSPDKEYEG
                     LSFTQGVYFGVDTKKGTMLYARAYPGNIMDIIGFDIDNFTRTVTDAKTLEIYTKYINI
                     PMVSIPSGCIHPKMMADTMHAMAERGYDYPVDFPRLIQEKRKEWEQIAGMPVAEVF"
     gene            complement(4989..5198)
                     /locus_tag="ACIE68_001913"
     CDS             complement(4989..5198)
                     /locus_tag="ACIE68_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFX5908233.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDVTLAAIYALGEGRCQAENITRE
                     LHRLSQSDDMEIKIAAIKALGRIYR"
     gene            5590..5766
                     /locus_tag="ACIE68_001914"
     CDS             5590..5766
                     /locus_tag="ACIE68_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908234.1"
                     /translation="MNLVDAFVKKVISEPYEEYGKWWIDVEYISWGVPGKTRLMFESK
                     EQALEVKEGYKFLT"
     gene            complement(5831..5926)
                     /gene="dqlB"
                     /locus_tag="ACIE68_001915"
     CDS             complement(5831..5926)
                     /gene="dqlB"
                     /locus_tag="ACIE68_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFX5908235.1"
                     /translation="MINLLIIVLRAVVAVANALIAVLELIRELVD"
     gene            6425..6676
                     /locus_tag="ACIE68_001916"
     CDS             6425..6676
                     /locus_tag="ACIE68_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908236.1"
                     /translation="MSEKWCKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAF
                     TLMTRRGICEMQNVAPDGKGIRLELIGFRENETIPDTLH"
     gene            complement(6748..6900)
                     /locus_tag="ACIE68_001917"
     CDS             complement(6748..6900)
                     /locus_tag="ACIE68_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFX5908237.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATL
                     AYEVKR"
     gene            7192..8400
                     /gene="trbA"
                     /locus_tag="ACIE68_001918"
     CDS             7192..8400
                     /gene="trbA"
                     /locus_tag="ACIE68_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HFX5908238.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLADGDPEKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPIYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            8419..9489
                     /gene="trbB"
                     /locus_tag="ACIE68_001919"
     CDS             8419..9489
                     /gene="trbB"
                     /locus_tag="ACIE68_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HFX5908239.1"
                     /translation="MTIEYFATRIAKHISVQAIWPDGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVPEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            9482..11773
                     /gene="trbC"
                     /locus_tag="ACIE68_001920"
     CDS             9482..11773
                     /gene="trbC"
                     /locus_tag="ACIE68_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFX5908240.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     RTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFS
                     IFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNH
                     PTGRPVNGFHHKKTNRPDWDGMY"
     gene            complement(11810..14509)
                     /gene="nikB"
                     /locus_tag="ACIE68_001921"
     CDS             complement(11810..14509)
                     /gene="nikB"
                     /locus_tag="ACIE68_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HFX5908241.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTD
                     TDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNP
                     DFIAADVRPEKIAETESLQQYACCHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFRQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
                     NALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG"
     gene            complement(14520..14852)
                     /gene="nikA"
                     /locus_tag="ACIE68_001922"
     CDS             complement(14520..14852)
                     /gene="nikA"
                     /locus_tag="ACIE68_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxosome accessory protein NikA"
                     /protein_id="HFX5908242.1"
                     /translation="MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            15086..15421
                     /locus_tag="ACIE68_001923"
     CDS             15086..15421
                     /locus_tag="ACIE68_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFX5908243.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(15507..16355)
                     /locus_tag="ACIE68_001924"
     CDS             complement(15507..16355)
                     /locus_tag="ACIE68_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFX5908244.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARKILLDASGDIFLYGFEDCVTDSVRRMDKQEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            16704..16901
                     /locus_tag="ACIE68_001925"
     CDS             16704..16901
                     /locus_tag="ACIE68_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908245.1"
                     /translation="MQGAPHTARLKKNDGTTMACGGHWHTTPAQVKTEKTARHLKAKK
                     VKFLHFFTLIYRADLAIVMIK"
     gene            16935..17186
                     /locus_tag="ACIE68_001926"
     CDS             16935..17186
                     /locus_tag="ACIE68_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFX5908246.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(17217..17462)
                     /locus_tag="ACIE68_001927"
     CDS             complement(17217..17462)
                     /locus_tag="ACIE68_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908247.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKQGALEV
                     AREIACRLRDIGWPPERIQEVTGLSGEELKKLFPDEQ"
     gene            complement(17462..18400)
                     /locus_tag="ACIE68_001928"
     CDS             complement(17462..18400)
                     /locus_tag="ACIE68_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFX5908248.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGRMEGRMEGAL
                     EKALAIACQLQKMGMTPEQIKQATGLSDDELKKITH"
     gene            complement(18465..18731)
                     /locus_tag="ACIE68_001929"
     CDS             complement(18465..18731)
                     /locus_tag="ACIE68_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908249.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(18823..19257)
                     /locus_tag="ACIE68_001930"
     CDS             complement(18823..19257)
                     /locus_tag="ACIE68_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFX5908250.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            complement(19993..20493)
                     /locus_tag="ACIE68_001931"
     CDS             complement(19993..20493)
                     /locus_tag="ACIE68_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFX5908251.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVEDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(20956..21552)
                     /locus_tag="ACIE68_001932"
     CDS             complement(20956..21552)
                     /locus_tag="ACIE68_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908252.1"
                     /translation="MMKSDEQYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLTWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSSVVREAQDWLCDRKLVDITDILR"
     gene            complement(21549..22268)
                     /locus_tag="ACIE68_001933"
     CDS             complement(21549..22268)
                     /locus_tag="ACIE68_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFX5908253.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPS
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            complement(22265..22699)
                     /gene="psiB"
                     /locus_tag="ACIE68_001934"
     CDS             complement(22265..22699)
                     /gene="psiB"
                     /locus_tag="ACIE68_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFX5908254.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNCGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(22754..24712)
                     /locus_tag="ACIE68_001935"
     CDS             complement(22754..24712)
                     /locus_tag="ACIE68_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFX5908255.1"
                     /translation="MPVTKCEPETTRKASRKSAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAGSIQGVGLLQNLVVHTLPGERYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTAAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNTKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEYLREAEGWGWCAGRMEAVGECREDARAYRNLPQPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQMQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDATERTDNLADAA"
     gene            complement(24771..25004)
                     /locus_tag="ACIE68_001936"
     CDS             complement(24771..25004)
                     /locus_tag="ACIE68_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFX5908256.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDHGEHFRLV
                     VRNNGALVWRTWNFEDGAGYWMNHAIRDFGILK"
     gene            complement(25062..25589)
                     /locus_tag="ACIE68_001937"
     CDS             complement(25062..25589)
                     /locus_tag="ACIE68_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFX5908257.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            25958..26026
                     /locus_tag="ACIE68_001938"
                     /pseudo
     CDS             25958..26026
                     /locus_tag="ACIE68_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
     gene            complement(26360..26551)
                     /locus_tag="ACIE68_001939"
     CDS             complement(26360..26551)
                     /locus_tag="ACIE68_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908258.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSINR"
     gene            complement(26548..26970)
                     /locus_tag="ACIE68_001940"
     CDS             complement(26548..26970)
                     /locus_tag="ACIE68_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFX5908259.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(27017..27442)
                     /locus_tag="ACIE68_001941"
     CDS             complement(27017..27442)
                     /locus_tag="ACIE68_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFX5908260.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(27860..28636)
                     /locus_tag="ACIE68_001942"
     CDS             complement(27860..28636)
                     /locus_tag="ACIE68_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908261.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLAMLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     FFPYAGEWLTEDEIRAVLDAVRDAVRSVSCRVAEDAQRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(28682..29116)
                     /locus_tag="ACIE68_001943"
     CDS             complement(28682..29116)
                     /locus_tag="ACIE68_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFX5908262.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGVAMEIINNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQAVINQARTLLKNNRHEND"
     gene            complement(29130..29351)
                     /locus_tag="ACIE68_001944"
     CDS             complement(29130..29351)
                     /locus_tag="ACIE68_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908263.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(29352..30035)
                     /locus_tag="ACIE68_001945"
     CDS             complement(29352..30035)
                     /locus_tag="ACIE68_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFX5908264.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMFRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIENF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(30111..30422)
                     /locus_tag="ACIE68_001946"
     CDS             complement(30111..30422)
                     /locus_tag="ACIE68_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908265.1"
                     /translation="MKYDGDGRATARFFSGKGCRHAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLPSERRRLRYSTVGLTRYRT
                     R"
     gene            complement(30419..31345)
                     /locus_tag="ACIE68_001947"
     CDS             complement(30419..31345)
                     /locus_tag="ACIE68_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFX5908266.1"
                     /translation="MPNWCSNRMYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLF
                     LAGSAGLLQITENIRSEQCPGVTAAGRGAVSPENIAFTRWLTHLQNGVLLDEQNCLML
                     HELWLQSGTGQRRWEELPDEVRETITVHFTAKRGDWCGFWSNEDVSVWWNRLCDNVLP
                     EKTMPFDLLTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQG
                     DNFIQVDFDTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLW
                     GELEWSSPTDDDELPEVTGPAWIVDKVAHYGG"
     gene            31739..31987
                     /locus_tag="ACIE68_001948"
     CDS             31739..31987
                     /locus_tag="ACIE68_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFX5908267.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            31984..32421
                     /gene="umuD"
                     /locus_tag="ACIE68_001949"
     CDS             31984..32421
                     /gene="umuD"
                     /locus_tag="ACIE68_001949"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFX5908268.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            32421..>33359
                     /gene="umuC"
                     /locus_tag="ACIE68_001950"
     CDS             32421..>33359
                     /gene="umuC"
                     /locus_tag="ACIE68_001950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HFX5908269.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEVFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRTLKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKL"
BASE COUNT         8092 a         9169 c         8478 g         7620 t
ORIGIN      
        1 aaggttatat caccaggcga tatcagtgtt tcccactggc cccactacct gaagacagaa
       61 aatacgacag ccttcctgac cttgtgctcc atcccgagca ggagacaata aaatgcaaag
      121 aaaaacatta ctcgcagccc ttatagccac cctgagtgga acggcatgcc aggctcatgc
      181 ttattcagtc accgtcgtcg ccagccggcc tgttgaagag caggtcatcc ccagaatgga
      241 agccatcaaa gatgtccttg gcaacatact gagtacccag acagccacgg gtaccgctat
      301 caatcagaac tctgaaaagc tggcaagtgt cattgcccag aacggacagg cgacacgtca
      361 gcagatgatt ttcagtaatg aaactcagcg cctcgaggag gcacggaaaa gcttcaccgt
      421 gccagactcc atctgcagtg aatccgcatc agggattgca acagaaagca aaagtgcctc
      481 agcatccgca gcctcaaaac tcagtaaagg tggcggtgtg agcaaccgta gcatccgcga
      541 ccgtctggcc agcgcagcaa actctcccgt gcgggaagct tatgatggcg cggccataca
      601 cgccagttac tgtacagaag cagaatatgc acgttttgga ggaaccgccg tttgtccttc
      661 tgtgggcgaa ataccaggtg gagacagcca ggtacgctcg atttaccacg gcgcaggtac
      721 agcggacaca cctgcggcac tgacatggga tcagaagcaa atagatgcag cgactgctta
      781 catgaaaaac acttcccgcc cctctgccgg tcgtgctctg ggtaaaggag aagtgaatac
      841 ccagagtggg cgaacctacg tcggactcca gaacgaatac aacggcatca ttgattctgc
      901 gtcgaatccc caactgacgc tgattgctga cagcacgcct aatgaaagca cacgtaaagc
      961 actggctgaa acactgcagt cagactcagc ggccgcttac ttcgaccagg tggcttcacc
     1021 agaagcaaaa gcacggggat atatgagtac ccgtgagttt gaagcttttg aggctggccg
     1081 gcgctacgcc aacacagcat acctggtgga tttacaggaa atgcaggggg ataacctgct
     1141 ccgtgaactg gtaagaatca ctgcgcaaat gaactggcag ctgaacgatc tgaaagaaca
     1201 gatccgccag ggtaatgtta tctctggcca gcaactggct ctgactgccc gccagtatta
     1261 tgaaaaacaa ctcggtagcc ttgagaaaac aatcaatcag gcaaacgcac gttgaaagct
     1321 gacgtcagaa caggactgaa aaaatgaata agttaccgga aaattcagac cagacgaaaa
     1381 acgtcaaaaa atcactatcc ataacccgca tagccacccg gggaattatt aacatttttg
     1441 ttcctctgat ggagactcgc ctgataacga attctgtaca gcatatgctg gaacaacaaa
     1501 aacgcaggct ggacagattt cgtcaggtaa ataataaaaa ggcgcaactc cacctcacgt
     1561 gggaagaagc tctgcaggcc agccatatgt cagtagacga tctggatcgt cgtttccgcc
     1621 ggcgcagaac agtatggcga ttctgttgct ggagtctgct ggccatcgct ttgtttctca
     1681 gtggtatgct ctttgccgcc tcttcattac ctcttacgac tctggtacgc gccatcagca
     1741 ctcttgtgtt gatactgtca ggcgtcgctc tttgtgcttc cagagcactg attgttacat
     1801 accgcctctg gcagttgcat gaacgaaaag tcagcgaacc cgagcagggg actttccgtg
     1861 attttctgaa cgaccgcaat ggctggcgca atgccacatt aattgccgta accagtaaac
     1921 aatactgatc acgtccagat gccagtacca ctggcatgtc gccatccccc ttaattaaat
     1981 cctgtgtggc ctttgcccat acgcaattct attccggagt tactgtgaaa attttacttc
     2041 gcgcactctg tgcaggcctt gctatatcct cactgccggc aatggcttca gtgacctatc
     2101 aggatattgt gtctgccgca accaatcctg acgatctgtc ccgtcaggcg ctggtcacga
     2161 tattcggtga tgtagtcacc aatccactgt caaccagcgc ccccactctg atcggaagca
     2221 tgttcggggc gttcaacagt atcatcgccg tacttgccgt tgtctggttt atgttcattg
     2281 gtatccgtca tgttgtgcgt tcggggcatc agggacaggt attcagcacc ggacgcgata
     2341 tcgtcggcac actgagtgtc gttgcaggtt ttctgatgat cgttccaacc ggaaatggct
     2401 ggagtatcgc tcagctcatt atgttatggg gcgcatcgat aatgggggtt gggtccgcca
     2461 atgtgatggt gcaacttgcc gctgataata tcgcaaatgg ttattcaatg actgtacagc
     2521 cagttcaggc atccacacga acagcagcgc gtggcatttt tgaaatggaa ctgtgtaagt
     2581 atgcggttaa cgcaggacta aatgatttta atcagacggt caaatcctcg acttcactga
     2641 tgacagaatc agctaaaacc gccagtggta attacactgt cacggtatcc aatggcagcg
     2701 gtatctgtgg ttcagccagc ctgtcagtag aaggaaacgg aactacagat caatcgacga
     2761 ttggaaaatt ctttaatccc ttcagtaaaa acgaatacag cggtgttatc tcagcacaac
     2821 gtgcagccat ggataatatg attagcgaca tggacaacgc tgccagtgag tttgtgacaa
     2881 catttctgga aaaaagaaat tctggcaacg gaacattgcc ggatatcgag acacggattc
     2941 agagagcagc cgacgagtat gaacgcgctg tacaaaaatc gctacctatt gataacggag
     3001 aacaatccag gaaagaagct ctcaagtctt atctcacaac ctatggatgg gtgacgcttg
     3061 gcgcgtggta tcagaccttt gccactgcta accagcgtct ggcagagctg gctgacagag
     3121 ctccagctgt aacttcaatg tcatcacttg gggaagttgg cgatactgat ttatttagtg
     3181 ctgttatggg ggcatacaag actcaattac agaactccac atatactcca acgataggaa
     3241 cgataacaac tcaagatgaa agtaatgcag ctaatagcac agacccacaa agtgtggtca
     3301 tgaaaagtat agcgccaacc atattaaaat ggacaaacca agctgctact gaatggtcag
     3361 gaacaggaac aaccagcaac caagttaacc cattaattaa gatgaaaaac atcggcgatt
     3421 atacattggg tacaacagaa attctctgga caggatatac aactgtcaga gttctcgcga
     3481 caatgggtga taactctatt ttcggaaagg ctgttaattt gttctctgga ttacctaaag
     3541 gatttgttgc tctgctggat gctgcagctc ccccaatata tttcttgttg ttcctgttgt
     3601 tttgtgcggg tttttcctta tcaatttacc taccatttat accattcatt ttctggatga
     3661 caggcattgg gaactggata gtaagcgtat taataggatg taccgccggg cctctgtggg
     3721 cagcaacaca tctaggaaca tcagaggaca gaggaagtcg agcagcttac ggatatatat
     3781 acttaattga cagcatgata agaccacctc tcatggtatt cggtttcttt tttgcctctg
     3841 tcgccatcat tgccgtcggc acaatattaa actcactgtt tggcgcagca ctagttaacg
     3901 tacaggtaaa atcattaaca gggatattca gccttgccgg gttcctgcta atttacgccc
     3961 gtatgtgtac aacgtctgta gctgcaatat ttgctttaca agcatatctt cctgaccatg
     4021 ttatcaattt cttgggtggt agagatggtg taaatactct tggcaacctc acctcatcag
     4081 taaaagatat ttttgcaggt tctaatagaa atattagaca cagccctgga gttagagagg
     4141 acagactaaa aaatgtcaca aaggatgata ataaagatgg aataaaagga taattttaat
     4201 aaaacaacct tgccataaac actcaatcgc attaaaatat cgatgagtgt ttatggaggg
     4261 tttattatgc aaatggtaaa aacaatcaat agatatcctg cttggtggac attatattac
     4321 gcccttagat ttatatattt cataagcata ccagtgttat ccatttttat tcttttcgga
     4381 gtgctctcca ttacatcttc aagatatgtg acgcaagaag attatattta tacttgcgtc
     4441 tgtctgtttt tactaattgc ccctgcaata ttgatgtatt cccgagcaag ctcaagaaaa
     4501 gacaaaataa aaaaaattgt cgcggagata aaaaacaccg gtttttattc acctgacaaa
     4561 gaatatgagg gactgtcttt tactcagggc gtctattttg gcgtggatac caaaaaaggc
     4621 actatgctgt atgctcgtgc ttaccctgga aatataatgg atattatcgg ctttgacatt
     4681 gacaacttca cacgcactgt gaccgacgcc aagacactag agatttatac aaaatacata
     4741 aatataccaa tggtctcaat tccgtcagga tgcatccatc ccaaaatgat ggcggataca
     4801 atgcatgcaa tggcagaaag aggatatgat taccctgtcg actttcctcg cctaattcag
     4861 gagaagcgta aggagtggga gcagattgca ggcatgcctg tagccgaagt attttaagat
     4921 aagctccctc cataacagcc ggttcaaatg agccggcttt tgttcattac aacagataac
     4981 ttatgccgtt accgataaat tcgaccaagt gctttaatcg cagcaatttt tatttccata
     5041 tcatcacttt gactcaatct atgtaattca cgagtaatgt tctccgcctg acaccggcct
     5101 tcacctaatg catagatcgc agcaagtgta acgtcttttg atttactctt catcatttca
     5161 aacaagaaag tattagctat cgcaggaggc atgctcataa tcaatcctct tcatgaaata
     5221 aacaatggca ctcacttaca aacagaagtc cactatcgca agcgactcaa cttagcaata
     5281 tctctcaatt tgaatgaaaa aaccagatgg catgtgacag ggaaccgtcc gcatgatatc
     5341 cgtgaccatt taaccccata agctaacctt gtcacaaacg ggcgcagaac agcctgccag
     5401 acagacaaca aaacaaaaat ccaagataaa acatgaatac cggtatctca atgcgaagaa
     5461 ataatctccg tagaaacccc catgagaaaa atatcatatg ggagaaaatc gcaattatca
     5521 cagccagcgc attcattatt ttcgctgtgc tctggcatta attattttca acacaaaaag
     5581 gaaaaaacaa tgaatctggt agacgccttt gtcaaaaaag taatatccga gccatatgaa
     5641 gaatatggaa aatggtggat agatgttgaa tacatttcat ggggtgttcc cgggaaaaca
     5701 agactgatgt ttgagtcaaa ggagcaggca ctggaagtta aagaagggta caaattttta
     5761 acgtgagatc tctttttgtt aaagggcagg aagcggtaac tccctgccct tagttacgtt
     5821 tccaggctta tcagtcgaca agttcccgga tcagttccag aacagcaatc agcgcgtttg
     5881 caacggcaac tactgccctg agaacgatga ttaacagatt aatcatccgc ttctccatca
     5941 ctggaggaaa tcaccgttgc ttactcttac attgcctgtc acaacggcac tttcagtgtt
     6001 agttgaaaag agacttcctc ctgccagagc actgaacaca gcaccacctg tttgttcagc
     6061 caggacttaa aatcttgcat ccatagtccc gatggatatc acggcagaag tgtcgccaga
     6121 cagagctaac ctgcggatta taaggatggt tgctatcaaa tgaacagctc gctattataa
     6181 gaacgttagt tgaaaagaga tttcctccgg tcagggccac ccactctgta acggaacagt
     6241 aaaaaaaccg acctcaccag tcggtttttt tacatctgtt gtttctcaaa atccgcccga
     6301 catgcccgtc acaccgagga aactcatgct ctgaatcaga accaatcatc tcaactttgc
     6361 cgtctcctga aaaaactggc acattcagtt cgataactac ccataaatac agagagaaaa
     6421 tccgatgtca gaaaagtggt gtaaagaatt cgactcttgc tttcaggtgg ggagcataga
     6481 agaaattgcc gatgctctcg agaaacgcag cggaagcgag gaaaacgcgt tagccatgct
     6541 ggcaatgaca gccttcacac tcatgacccg acgtggtatc tgcgaaatgc agaacgtggc
     6601 ccccgatggc aaaggcattc ggctggagct aatcggcttc agggaaaatg aaacaatacc
     6661 ggatacgctt cattgaagaa gaaagggcgc aatgaggtca gccttcgcaa caaagcggcg
     6721 gcagaaaacc tgccgccgtg ttgcccgtta acgtttaact tcgtaggcta acgttgccac
     6781 aagcactgtg tttccctgct ggagccggag tccgcaaagc gaatccctca ccatccagac
     6841 aaaacacaga atcgtcacac agatgacgat taacatcatt aaaaacgttc gctgtggcat
     6901 catagcccct ccattgcctt acggcggtta tcgggctact ataatgttgt ctaggcatta
     6961 cagtagagcc cttggttgat tgataaaaaa atctccaggg gctttctgct gtataccgtc
     7021 cacaaaacgc tttcggcata cagcctcaag cgcccgcagc tattctaacg actttcgtat
     7081 ctgcaacaat ccttttatcg cacggctaat tcctctggtt agctcttatc gcctgcaaat
     7141 aaatttttaa cctttccgtt tgatttaaat tccagaaatg gagactttgt catgtcttac
     7201 aatcgacagc ctgtcgcaga agatcctatg cagatatggg gcgctgttgg tgtccttctt
     7261 atcctgctgc tattcgttat atggttattc ttgccggaag tggtttacgc cagctgcctg
     7321 attcttcata ccttatgggg actggttgac tggggaccat ttcataacta tgcagcacca
     7381 cgatacaacc tgcttgccat gaccggtaac aacgccgcca acataagcta tagccagtgg
     7441 gttaacgtca tggagcagac cataggtatt ctgtggatgt acctcttacc tgtgacactc
     7501 tggtgtttgt gggagtggta tcaacatccg ggacaaagtc gtttcacccg cagaccagta
     7561 gatattaccc gactgccaca catctttgct tcgttgtccc ctgctatcgc ccctgtactg
     7621 gcagatggtg atccggaaaa actctttcat ggtggcaaac gacctgaacg gcgggttgct
     7681 ctcaccccgg aagcatttgt cgagcagcat actctcatca ccaatatgca gctcgatgtt
     7741 gcagctgcaa gacgatgttt catggcccag ttgggtaaac ctttaacgtc ctggaaagac
     7801 atggcaccac atgaaaaagc gctctttgcc atatttggtc ttcagtattt cctggatgat
     7861 cgtaaggcag cattaaagct catggataca ctgaacctct catgcagaat taaaagcaaa
     7921 cgggacagtg ggaaattctg tacccctatt tattcactgg ccaaatcagc tttccagaga
     7981 gtgattaaaa gcaacggagc acagcaatgg ctgaaacaac atcgctacgt ccgcagtggg
     8041 ctggtctggc tgtatgcaca tgacctgcgg cttacgccac ccaactggat ttggctgaaa
     8101 ggcgtggaca ggacgctgtt ttatgcatta caccgcgcca ataccacgaa aggatttatt
     8161 gaaggcgctg gcgtggtggc cgttgcacgt gcagaggccg aagccatgcg ttttggtctt
     8221 ccctgccctg aaccctgtgt tgatgaagcg gtggaaggct tacggcggga tatgctcagt
     8281 ctcggtctta tctgggatga accacaacca gatcgcgacc ggaaacgccg catcttgacc
     8341 aactggtccc tcaccgacga catcctgccc cgcacacctg ctacagacaa cgaattctga
     8401 ttatccggag aactgaaaat gacaatcgag tactttgcaa cacgcattgc aaaacatatt
     8461 tccgtgcagg caatctggcc ggatggcagg acagaaatta ttgccgtcct tccacgggat
     8521 gccctgtcag ggctggtaac acgaaataat caactgattc atatagcctt tgacgccatg
     8581 ggcacccgta acagagaaac cgtcctgatt gataaacagg agcataacgc tgaccagata
     8641 cttacagcta ttgattcctg cctgcgcctt gaatatctga tggaacgccg cttcagcaga
     8701 acacctttgt tccgtggcat aatcgccagc gttgtgctgt tcgtaatggc aacgattggc
     8761 ttctctctgt tccgctatgt tgatcgtgta ttctgggatg atacaacgcc tgaagcagta
     8821 cagacagcag gtgagccacg actgctaccc cctcacctga atcacactgt gccattaaat
     8881 gaaggcattc aactcccggt tcccccgaaa aaagacgtcc aggtcccgga gaaaacaatc
     8941 acaagcaaaa atccggaagc cgcagctgcg cgacataatc tggctactgt tctgaagcgg
     9001 aacgccgatc ggggcatgtt taccatcaac ctgtcatccg gtcatgaacg aaccctgttc
     9061 gcatttctcg atcctgcctg tcctaactgc agacttctgg agccggcctt aaaacgcctg
     9121 gccagcgact tcaatgtcgt tatctacccg gtttcagtta ttggtggaga ggaatccacc
     9181 gaccgtgtgg caccactgct gtgtgaaaaa gatgctcaga aacgtgctgc cggctggcac
     9241 cgactgtatt ccgcagataa cggaatgatg acgccatcag aagaaacaac gcctgcagat
     9301 gaaacatgcc tgaaagcagc cagagctgcg attgatgtta acaatgtcgc cttccgtaag
     9361 ttcggatttg ccggtacccc atgggtgctg tccgacaccg gctggcatct gcctacagga
     9421 attctgcagg aaactggcac gcttaattta ttcctgaaaa ccaccgacag cgagtctggc
     9481 catgagtgaa catcgcgtta accccgagct tttacaccgt acagcctggg gaaatcctgt
     9541 ctggaatgca ctccagagtc tcaatatcta tggcttttgc ctggtggcaa gtcttgtggc
     9601 cagttttatc tggccacttg cattaccggc atgtctgttg ttcacactga taacaatgct
     9661 ggtatttagt ctgcagcgat ggcgctgccc tcttcgtatg cccatgacgc tggaatgtgc
     9721 tgacccgtca caggacagaa tgattaaaag aagtcttttc agcttctggc caaccctgtt
     9781 ccagtatgag gtcattctgg aatcaccggc cagcggcata ttctatgttg gttatcaacg
     9841 cgtcagggat attggcaggg agttatggct gagtatggac gatctgaccc gccatataat
     9901 gtttttcgcc accaccggtg gcggtaaaac ggaaaccatc tttgcctggg cgattaaccc
     9961 actttgctgg gcaagaggat tcacccttgt cgatggcaag gcacagaatg atacggcaag
    10021 aacgatctgg tatctggcca gacgctttgg acgtgaagac gatgtggaag tcattaactt
    10081 catgaacgga ggaaaatccc gcagtgaaat cattctgagt ggtgaaaaaa cgcgtcctca
    10141 gtcaaatacc tggaatccct tctgttacag cactgaggca ttcacagcgg agacaatgca
    10201 gtcaatgctg ccccagaacg tccagggggg cgagtggcag tcccgggcca ttgccatgaa
    10261 taaggcactg gtatttggta ctaaattctg gtgtgtgcgc gaaggcaaaa caatgtcgct
    10321 gcagatgctc cgtgaacata tgactctcga agggatggcc aaactttact gtcgtggcct
    10381 cgatgatcag tggccggaag aagctatcgc accgttacgt aactaccttc aggacgttcc
    10441 cggtttcgac ctgtcgctgg tcaggacacc atcagcctgg acggaagaac ccagaaaaca
    10501 acatgcttat ctctccgggc agttttctga aactttcagc acattcacgg aagcattcgg
    10561 ggatattttt gctgaagatt ccggtgatat cgatatccgt gacagcattc acagcgacag
    10621 gattctgatg gtaatgatcc ccgctctgga tacatcagcg catacaacgt ctgctcttgg
    10681 acgaatgttt atcacccaga aaagtatgat tctcgcccgt gatttgggct accgcctcga
    10741 aggaactgat tccgatgcac tggaagtcaa aaaatataag gggcgtttcc cttatctgtg
    10801 tttccttgat gaagtggggg catactatac ggaccgtatt gctgtggaag ccactcaggt
    10861 gcgttcactg gattttgctc tgattttgat ggcacaggac caggaacgta tagaaggaca
    10921 gacaacagcc acgaatactg caacactgat gcagaacacg gggacaaaat ttgccggcag
    10981 gattgtcagc gagggtagta cggccagaac gctgaaaagc gccgccggtg aagaagccag
    11041 ggcgagaatg aacaacctgc agcgtcagga cggcatattt ggtgaatcat ggattgacag
    11101 cccacaaatc agcatcctga tggaaagcaa gataaatgtt caggagttga ttgaacttca
    11161 tcctggcgag ttcttttcta ttttccgggg agaaaccgta ccatcagcat cattcttcat
    11221 ccctgacgat gagaaaagct gcagcagcga tcccgttgtt ataaacaggt atatcagcgt
    11281 ggatgcgcca cgcctggacc gtctccgccg tcttgttccc cgtactactc aacgtcgaat
    11341 accgtcaccg gaaaatgtca gtgccatcat tggcgtcctg actgcaaaac cctccagaaa
    11401 aagacgaaaa attcgcacag agccgcacac gatcgtggat acattccagc aacgcattgc
    11461 aggacgtcag gcggctatgg caatgctgga agagtacgac acagatatca acgcacgtga
    11521 aagtgccttg tgggaaacag cggtgaatac gcttaaaacg accacacggg aagagcgacg
    11581 aattcgctat atcacgctga acagaccaga actacctgaa acaaaagagg aaaatcagat
    11641 atcagtgaga gcagaaagag caggtataaa cctgttaacg cttccacagg acaataacca
    11701 cccgacaggg cgtcctgtaa acgggtttca tcataaaaaa accaaccgtc cggactggga
    11761 cggaatgtac tgaacagtgg ccgcgccgga ttctggcgcg gccttttttt taacctggag
    11821 aaggcggttc ccagcgttta tcctgctccg gcggaggcag tgatgcgtca tcgttgctgt
    11881 tcgccgcgat ataatcgcgg tagtagcgtt cacttccttc acggtgatga tccacgtccg
    11941 gtcgcgtaat cctgtagtct tcgtgaccgg tacggcctgt cacattatgc caggcaacca
    12001 tttcagcaaa gacctggtta aattgtttgt cgtttccttc tggttcaaaa cccatgcgtg
    12061 gatcacgccc gaataatacc ggtgcaatca aatccagttt atctgccagc gcattgcggt
    12121 catggtgatt acggaaaaca accctgtcac cataatccgt acagacaaac tctcccgtcc
    12181 gacggtcacg gaaacgaaca ctcccgttct tctgcagacg ggcctcgaga tcaccggtat
    12241 taccgtatac cggcgttccg cccggttcac aaagcacgac acagcggtct gtcgtcgttg
    12301 tgcgtgacct gtctttacgg cgatcgcgat aatcccagcc tctcagctgc gatacagcag
    12361 ccctgtctcc ctgagcagcc tgaatttcga cccactgacg gtaagagggt ggataccatt
    12421 tcccatcagc aataagtttc tgtcgctcat ccctgatgtc ttctttcagc ctgatcaatg
    12481 cctgcattcg ctgaacttct gcaatgtgat aatgcagctt acgtaatgcc ggatcatcgt
    12541 actgcgcacg aatgtgtgac ttccgcagac ggcatgcctg atgaatttcc cgacaacgtt
    12601 ccccataacg cagatctggt ttacgccatt gttcacgcca tgccagatag cgggcacgca
    12661 ggtcagcacg ggcctcagct ctggcttcac gtcgctgccg gcgcagcatt gggtcacgtt
    12721 tactgctgac cccgtacctg tcactgacag ccagctccgg gttatagcga ctttcaggtt
    12781 gcaccctgtc aaacaaatct gctggggcac tcacaaaagg tccggcctgt ggctctgcac
    12841 gccccagtgt cagatcagga tgaatgctgc tggccttcac cggcgtttgc tcatgattaa
    12901 aggcatcaac gacaacaagc ccgtgatgtt gtttctgtag catcagtccc tgttgtgcaa
    12961 acagctcatg acaacgctgc caggtaaact caccctgtgt gatcatttct tcaatctgag
    13021 ggctacaatg gctgatgctg tatccccaca gactttcttt gtcagaaaac agttcgcgtt
    13081 tgatagcccc ctgcggcccc gtgcctgtac gcatacggtg ccattcttta tcggtcgcag
    13141 gacagctctc catacgacga ccacggaagc gttctgttgg cgtaacctgg cggaaaatat
    13201 ctgttggtac aggctgccag ccaccatcga gttgattcac attgtcgagc gtcagcagcg
    13261 accatacgct gtcagcacga acgggagtct ggttatgatc ataaccgtca cagataacca
    13321 ggtgaccatg ctgaacacgc atccataatc ccgcttcagc cagtgtgcgg tgaatagcct
    13381 ggcagttttc cagccgtcct tcccgtgcca tttccggcag acgttctcca agatgacaac
    13441 aggcatactg ctgcagactt tctgtttcag cgattttttc cgggcgaaca tcagcggcaa
    13501 tgaagtccgg attatagcgt cctggtgctt ccacctggct gaaaatgtct tttggtactg
    13561 gcgtgtagtc acccattttt ttcatgagtt tttctgcaca ccaggaggga ccaaacgagt
    13621 caagctgtac accttccctg ttgcgatccc agccatccat caccagaaat tttccgtcca
    13681 tctgagacag gtacagacca tcttccgcaa gacgacgatg cagggataac caatcattta
    13741 caggttcact gcgtaaacca gcgaccgctg tctgcgcgac atactcgcga aaagtttgtt
    13801 ttttcccgcg tgtccacgcg ttctggcgat cacgttcaac ggcggttttg cgaacgatac
    13861 gatttcccgg tgcatggacc cagcaaccgt tatccggagc aaaaccatgc ttcagttcaa
    13921 gctcacggca ggcacgactg agtttttcct gactccagga taaccggttc agataacccg
    13981 tttcagggtg tacccggtta acagccacat ggacgtgcag attatctgta tcagtatgca
    14041 cggcagagac atactgatgg tcggcaaggc cgagtgattt cagcgtatga cgtacactgt
    14101 cataaatctg ttccggacgc gggctttcat gtgactgcca ggaaaggatg tagtgaaaaa
    14161 caggatcagt gtcatctttt gcatagtgtg cctgccgggc aatgtactcc atatctgcag
    14221 cggcagtttc aagagaagta cagttatgaa agcaggtgac accataaaag ttgacccatt
    14281 cgcagccgtc cttcatgaca tcaaccagcg ccacgaatga ctcatttcga agacgtgtcg
    14341 cataatcaac aaggcgactg aaacggcttc ggtgcggttg ttcagcctgc gaagaagaag
    14401 ataaatccag ttcctcatcg gtcatgtcat cccggacaga gacgtaggat accaggtctt
    14461 cgaacgaaga cttgccgtcc cttctctttt tcggaatgac tgcattcacc ggagaatcct
    14521 caaccttcca ttaactgttt acgcaatgta tttgtaagtt cggttatggc aacaagcact
    14581 tctgcgtatt ccttgtcacc ggttcttttt ccctgaacaa acagatgctt ctgcatcctt
    14641 cccagtctca ttaactcctt cagaaaagca tcatctgtac gggagttaat tcgcttattc
    14701 agtgctgcat ttcggatgta agcagataca gtaagtccgg aatcttcagc ttttttcctg
    14761 atggtttcat cctcaacagg agagaatctg agtgtcctga caactgtctt ttgccgtacc
    14821 tcacttttct ttcttaccgc actatcactc acttcaggct ccttacgggg tgtcggggcg
    14881 aagccctgac cagatggcaa ttgtaatagc gtcgcgtgtg acggtattac aattgcacat
    14941 cctgtcccgt ttttcaggcc attatagcct gttatttttg tgcatcatag tcttatgcag
    15001 acggcagagg cagggcgcag ccctgccagg agtcagtttt tgatgataaa atcagacttc
    15061 aacatgaaac ggagagaaag tgccaatggc ttctaaaaaa ttttactcag atgatgacat
    15121 ccagctggca aaagctgctt tatcggaatt gccagatctc accgctcaac ggaaaacatt
    15181 gcgtgatttt cttgatgcca ttcgtgatga cattattgtc ctggtaagga caaagggtta
    15241 tacactggca gatgttcgtg acacgctcaa aaatgcaggg tatgaagtag gagagaaggc
    15301 acttcgtgac attattcggg aagcagaatc gaaaaaacca tcccgccgtt cttcctctaa
    15361 aacagcacct aaaaagacag attcaggcag gaaagatggc atcgatatga acaacaattg
    15421 atgtgacaat gtcacatcgt gttctgagat tatgatgatt acagaaaaaa aagccccggc
    15481 ataaatacca gggcttttct gtttaatcag actcgtggcg gcaatgcacc tggataatgc
    15541 cgtgcaataa tatcattcag tttttcaaca aggtcagaac gcttaaagac aatgtgtacg
    15601 gtccctttct ggtagccacg caccgtaaaa tattcccctt ctaacagctt accgacattg
    15661 tcgccgttcc cgagaaattc accatactgc gccccgatgc tgttcctgaa gtcaggaacg
    15721 ttgcgcccct ccagcagata aaacggacgt accaggtcat ccagtgcctc ccgtccgctg
    15781 cagtccagcg tcacacgccc gttactccag cggtacagca ggttttcaat aatgattttc
    15841 ttacccagac ggcagggatt attggttctg taatcccatg acaatttccg gtagacatca
    15901 atcagcccct gttcaaacgt ctgcatcttg ctggcattaa gatggcggaa tgttgccagt
    15961 acattgtcca gggtgatttc aggacaggtg tcgctcatca gctggctgtt ccactcatca
    16021 cgctggcatg aactcatgaa ggtgtacatg cccgtatctg tcatcaggcg gtcccagatt
    16081 ttccggtcgg caagacgggt gatattcctt ttcgcttctt cctgcttatc catgcgacga
    16141 acggaatcag tcacgcagtc ttcaaacccg taaagaaaaa tatcacctga cgcatcgagc
    16201 agaatcttgc gcgctgtcgc caggcattcc atcgcctgca tgaacagtgc aataccttcg
    16261 ttacgctgcg cgatgatgcg gtcgatagcc acagacggaa taacatctgt cgtgccggca
    16321 gtattcaggt cagcagtggg taaagtctga ttcatcacat tgtctcctca tcgtcacgac
    16381 tgccgggtca gccaggtcat ttcccggagg agcgaagctc cttctgccgt ccgggtcttc
    16441 ggggatttcc ccgcagtgga cctttccgac gggggacgct caccacgtca cggggcagtt
    16501 gtccgggggc agacgcaaaa gtttttgacg cctgcggcaa aaagttttgt ggctggtaca
    16561 tctctcccct ggacgactga aacgggatgt ggtcatgtat gtacccccgg aaagtccgtc
    16621 tgcggagaac aggccgcagt gcggcggcag acaggcagag acagctgcgc catcaggcgc
    16681 agtggcctta cacaccgtaa ggtgtgcagg gagcgccgca tacggcgcga ctgaagaaaa
    16741 acgacggcac aacaatggca tgtggtggtc actggcacac cactcccgca caggtgaaaa
    16801 cagagaaaac cgcacgacat ttaaaagcaa aaaaagtgaa atttcttcat ttttttacac
    16861 ttatttatcg cgctgatttg gcaattgtga tgataaaata aagtgaagtt ttttcacttt
    16921 ttcaaaggtg ttttatgacc ggctgggaac tgcgaatctg gcgaaaaagc atgctctggt
    16981 cacgcgaaaa agcggccaga gaatttggcg tgacgcagag aacatggcac gcctgggaaa
    17041 atgcggaaca ggtggacgtc accgtctgga ggaccaccca ggcactttca gtccgtgacc
    17101 tgctgcctca catgcagggg atgcgtaagg cagatatcat caggcgactg gaaaatgaac
    17161 tgggggaaac ggcagggaat gtgtgagcgt gattcacagc ctggctgaat gccaggctac
    17221 tgctcatcag gaaacagctt tttcagttct tcaccggaaa gtcccgtgac ctcctgaatc
    17281 cgttccggcg gccagcctat atcccgaagc cggcaggcta tttcccgcgc cacttcaaga
    17341 gccccttgtt taaaaccctc gtcgaaacct ttctgtataa gacgttctgc aatcgtcatg
    17401 ctgtttgctc tgctcctgct gacaaaaaag cccggcattt tgccgggctt tgtggttctc
    17461 atcagtgagt gattttcttc agttcgtcat cggaaagtcc ggtagcctgc ttaatctgct
    17521 ccggcgtcat ccccattttc tgaagctggc acgcaatagc cagggctttt tcaagcgctc
    17581 cttccatgcg tccttccatg cgtccttcca tgcgtccttc catcctgcct tcctggcgac
    17641 ctttttgctc aagacgttct gcgatagtca tcagtttatc ctcatgctgc ggcagacgct
    17701 cagccagcgc ccgcataaat tccggtgata cagagtcccc tgcgttaacg atgtaatgaa
    17761 tcagaacacg cacctgctcg tctgaaattt ccaccatgac ctgcgggagt ttgtccagca
    17821 gctccatcat gtcacgatgg cggatatgct tcatcagcag cgtcagtgcg gccatccggc
    17881 gatggttcat gatttcattg tcatcaacga cagtgatatc aaccagcgga aacggctttg
    17941 tgtatatttt agccgcaagc gccggattct caaaacagtc cagccagttc atgctgtacg
    18001 ggtacgggct gcgctcaccg tgatagaaca gcaccggaat gaccagtggc aacgttttgt
    18061 gctgctccag atgtctctgc atcgccgcca cactgtaacg taacatgcgg aaaggcatgt
    18121 acagattttc ggtgctctgg tgctcgatga gcgtataaac atagccatct tcacccccgg
    18181 tggttttcac gctccagagg atatcgctgg cgtactgatg caggtccggc tcaacaaagg
    18241 tggcgggttc cagcttcagc gtggacaggt cgcacaactg ccggtactcc gcaggaagat
    18301 gcagttccag aaaatctctg gcgacgtcgg gattcgccag gaacgaccgg aacgcggcat
    18361 catgcggcgt gggcgttgtg gtgtttttct tcttactcac ggttcctcct ggtgagatgt
    18421 ctgaacgtat cctgccgcct gcacaggcag acggcaaggg ttaatcagtg gcagacccgc
    18481 tcaatttcaa gcgcgcggag caggtggccc cattcgctga ctttcatagc ggggatacgc
    18541 acccgcttct gctcactgca gcgtctggcg atatggccgc tcatcgccag gacgtccggg
    18601 ttaatctcat cctgtgccag ctcctgacgt ctgaagcggg tatggcggtg caggggttta
    18661 ttctcccgca ggcactcttc acgcagacgc tttacatact cggcggttgc gaagataatt
    18721 gatggtctca caaatatttc tccttatgtt gttgatgact gccgctcttc ctggcgggga
    18781 agtaacggcg gagagaggca gggtttatcg ccctgccttt tttcatttcg gacagcgacg
    18841 cagtgccagc gcgagcgctt tcatcttcca gccttccggc gtgcggtaca tcaccttcca
    18901 gtagccgtcg atgaacacat aatggaagcg cacctcttca gtgctttcag tggcaatagc
    18961 ctccctgaaa gaacggtact cacgcgcctc actgtccatc cggaaaccac tgtcacgtcc
    19021 gtaataagcc gtcacaccgt ccaccgggtt ctgaagcgta tgcccggcgg gtttgtcgca
    19081 acggggaccg aggcaacgga tatcaccgtg ttcaaccagg gcttcggcct gctgaccggt
    19141 ggcatagtgg gtatgcagga tgcgaccgtt ccaggtcggc tccccgtgca ggtggcagta
    19201 aacggtacgg aacaccccgt cgcggcaaaa aacggaaatc gtgctgctga ctgtcatgaa
    19261 aacgtctcct tctgctgaat caggtcgccg ggccgaccgg ttgctccccg gaggggcgaa
    19321 agccccttct gccgttgccg tccacttctt cggggtttct ccccgcagtg gacctttccg
    19381 atggggcagc gacagccacg gcacggggca gttgtccagg ggcagacgca aaagtttttg
    19441 acgcttgcgg caaaaagttt tgtggctggt acatctcccc ctggacggct gacacgtgtt
    19501 gtggccgctt cagcccccgg aaagtccaca gcgggaaaca ggccgcaggg gtggcggcgg
    19561 aagggcagac aactgcgcca tcaggcgcag tttccttaca caccgtcagg tgtgcaggga
    19621 gcgcaggtca cggcgcgaca gaggcggcgc gaccggaacg gtcgctgcaa gccaggtttt
    19681 tcagacatcg gaccgtagcg gcagcccgaa accgcgccag cgggttcggc ggagactggc
    19741 gggtgcgcca gcagctgcct gtcggagtcg ggcctgacga ccggatgccg ccaggcagcc
    19801 gggagccgaa ggtgtccccg cctccggaaa tcagaacaac agaaacgaca taacagaaaa
    19861 atgcctcaca ccatgacggg caggaagggc agcgccgcag gcgcggccct cctgcatttc
    19921 aggcaggcgt aagcctgcca gggttttccg ggggcctggc aggccatacc cccgccggag
    19981 cgggggagta aatcaccgac ggaacacatg accgtcaata aaggtgaagg agtccaggaa
    20041 taaatcccgc gcatacgcct catagtcaaa atagagcgcc accgtctccg gcacgtcagc
    20101 cagcaggccg gtatcactgg cgaactcaac cgcaaaagcc tcctcactgt cagcctcgcc
    20161 ccaccaggca tcgcggaagg tgtcaaaatc cgtctcaccg gtatcctcca cccagagacg
    20221 ataagcctct tcgcagcctt catcccgtgc ctggcggaag ccttcaaccc aggcccagtt
    20281 gatatggcat tcagaggcca tattccccgg gaatccctca taatcctgaa acatcagttc
    20341 aggatcggct tcatcctggt gaagagcacg gcaggcggcg aaaaagtcgc gctcatcatc
    20401 aaacgtggtc aggtcaaacc agcgtccggc gatgcttcca cagttgtatt tgtgccaggt
    20461 tccaacgtat acagcaggtg caacaacaga cattcccgaa tcctccatag tcacatgttc
    20521 tgcggcaccg ggcggtgccg tgcctccata aaagccgggc gataaggcgg ggatgcacag
    20581 ggcgcaaggg aagccgcagc ctgacgcaca cggctggcgg gagttggagg gtgagaccgc
    20641 gaaatctgag cgggtgaacg accggaaaat cacggcagac gggaaggagg gaccgggtga
    20701 ccgcagcggc gaagcgtacc cttgcatccc tgccagcccg gctaagatgg aggcacggca
    20761 cagcccgcac acgacgctga ggccacagag ctgaaaaccc acaaggccat gccccgcatg
    20821 gccgggtaaa agcggagccg ccggcgactt ccttattccg gcgagtgcag aggacacgcc
    20881 gcatgacctc cagaccaccc ctgatatgaa acgatcatga tcggcgtgtc cctgcgtaaa
    20941 atatacatgt ctggttcacc ggagaatatc tgtgatatcc acaagttttc tgtcgcacag
    21001 ccagtcctgc gcttcacgca ccacgctact gtcgggtttc accgctttca gcgatgatgc
    21061 cagtcgcttg atcaacataa ccagttcccg tgtctgtatg tccggcattg tctccggcgg
    21121 agcagaggga ctctcatttc cgacgcccat cccatgcgct tccagtgtgg cacggacaaa
    21181 ccaggcggca tcgaccagtt gctggcgaat ccaggtcaga tcggcatttc tgtcacgggc
    21241 tgccattcgc tcacccgaca gcgccttgct gatatggctg cgcaccagat tatccaggag
    21301 ttcctgcgct tcttccggtg aaaagccacc tggtatctct gtgcgcaaca gtcctttcct
    21361 gtacagtagc tgcagcagat caatctgcga ccgcactgca actatcagta cataacggac
    21421 aatattcgca ctggcggtgg tttctgtatc attcagcagt tcctccggat cattaccacc
    21481 ggtattgcag agcaacggca acagaggtcg catccacacg ggaacctgat attgttcgtc
    21541 tgatttcatc atattgcgtt ctccagcttg ttcgggatca tccactgctc aagtgcacgt
    21601 acctgtaccg gtgcaccacg accggcctca ccagcttcaa aaatcagccg ccagagtggt
    21661 tcctcctgcc agaaaaaccg gtcaaatgat gacagtgatg gaaaacgccc gcaccacccc
    21721 cagaaaatcg tttccagatc atgctcctca acaacaccag accagcggga caaccagctt
    21781 ccgaccgtgg agggagtatg aaacgcaagg tcaatctgcg cctgagcaaa acatgtctgg
    21841 tattccagtt cccgtcgttc tgcctcacgt tcccgctggc ggctgtattt ctgctctgtc
    21901 agcgtggttt tctgacgacg ccgctcacct tcacgaaaag gaacggacgg gaacagacga
    21961 tgccccgcat cggatggcag gggaagaaca cagacttcac caaaactttc aatcagttta
    22021 tccacggcag ccagccacag gagacggttg ccatgaaatt cctccttcgt cagcgcagga
    22081 gcaaaaaagc ggagatcggt cagcgaaatc cggcgactgc cattcaggca gcgaaagaaa
    22141 gcgctggcat aaggccatgc tgaaagtgtg cgtccctgac gacgacgttg ttcggtgaca
    22201 gccacagcct gcatggcggc ctgttgctct gcgctgaggg ggatcagtgc ccgtgaacgt
    22261 gcgctcatgc ctgacctcct tctgccatca ggatatggat gatatcgtta acactgtacc
    22321 cctgcgcatc aagtgtcgcg gcaagcgcca gcgtgtgact gatagcgaca ggcgtgaaga
    22381 tatgttgcac ctgaacgacg gcgaaaggct gtccaccaca attcaccaga accagcatcc
    22441 agagctgaga gacgtctccc ggcgaacata atgccaggtg gaaacgttcg tgggctggcg
    22501 tgaaacgaac ctggacgggg aaaaagcccc cgaactcact gccatactcg ccattcatca
    22561 tccagttagc cggtgcgtcc acttcacgca tcacctcgtg agtgagatta cggcgcatat
    22621 cgcttcccgc agcacggata tcttcatatt cctgtgcgtt catggactgc agggcattca
    22681 gggtcagttc agttttcatc acggtatctc ccttgctgcc gccccgtctc cggcggggcg
    22741 gtgtggttgt tcatcaggcg gcatcagcca ggttatcggt gcgttcagtg gcatctgtct
    22801 gtggatgtgg tgcacacatc cagccaggca cccagcggtt gtctttcata tggtgctctg
    22861 catgttcagc cgcatcacct ttcttcatct tctccgcgtc ccgtgcggca ccggacagtc
    22921 ctgcctcatt cagggctgcg ataatctgcg gctttttcag gtgtccgaag aagtttgctt
    22981 ttgtcggctg ccaccagtcg cgcatgtgaa agccgatggc gctttccagc gaatccagcg
    23041 gactgcgtga cgtgtgacca cactcacggg tctggacacc gttaaggctg catgcggtgc
    23101 agaaactgag cagggataac agcacctcct gactgagtga caggaacgtc gtcatgtccc
    23161 gtgaccatcc ctccggtaac agggccgcaa gacgggcttt ttctgccatc atcgccatga
    23221 acgcggcacc ttccttccct gacggcgcat cgctggtcag cgaataatgt ttacattcca
    23281 ggctgatttg tgctggttta ctgtacgctc cgctgccaaa cacattcagg cagagcgtcc
    23341 atgccagcag tgccagagat ttgtccggtt gctgcatgag tgccgcctgg actgccagcg
    23401 tgcgttcgga agacattttc gtcagcagtg gcagactgat ttcctccact gacgctttct
    23461 cctgcatctg ttccgtgcgg tcagcgtcgt cagccacgtc atcctcactg cgcaactgca
    23521 caccacgctg gacacacacg ttgccataac gccaggacac caccacaccg cttccggcac
    23581 gcatctccgg cgtccacgct ctgaccttcg ccatgcagcg catcagcttc atttctgctt
    23641 ccagcaggtc agattcctca cactggtttt ccagtgcgtc gtaacgtgcc atcagttcgt
    23701 tcaggcgttc ttcttccgcc tccgtcagca ctgcttccgg ctgcggcagg ttgcggtatg
    23761 cccgggcatc ttcacggcac tcaccaacgg cctccatgcg tccggcacac catccccagc
    23821 cctcagcttc ccgaaggtac tctgcaacag cctggagttt ttccagcagg gcggcatcga
    23881 gcgcaacaca gtccacatag ccaccctcat cgtcgctgaa caaatcagtg cgcagttcat
    23941 ctggcgagaa ggcatcagcc cccacgaagc ggaatttagt gttccccgcc accgccactt
    24001 cactttcggt caccagacga cggatggtct gtacttccgg ttttcctccc catcccgact
    24061 gacaggcggc ttcaaacacc tgcacctgac gcgcggtgtc gttctccagc gccagcgcct
    24121 gacagtgctc tgtggtgatg cggtcttctg ccagcgcatc aaggatgaca ggcgcaaggt
    24181 ctgccagttt cagcattcgc tgaacgtggc ggggtgaata gcccagcaaa tcaccgattt
    24241 gtgccgctgt tttgccttcc tgcgccattg cgcggaatcc ggcaatctgt tcggcagggt
    24301 gcatatcccg gcgatgaccg ttctcggtca tcgatgcagc cgtcgccagt tcctgcggaa
    24361 taattttcac gcggacaggc cagtcagccg gaatgattcc acgctcagcc agcatattga
    24421 gtgctgccag tctgcgacca cctgcggcga caccgtagcg ctcaccaggc agggtatgca
    24481 cgaccagatt ctgcagcagt ccgacgccct gaatggaccc tgccagttcg ctgacggact
    24541 ccgcagaata cggcaccgtg cgcacattca gcggtgactt aatcagtgag gccagcggca
    24601 cgctcacttc ctccgtctgc gccagcaggg cagacaggac tgtttcctgc gtttttgcag
    24661 atttacggct tgctttgcgg gtggtttctg gttcacactt cgttacaggc ataatcatcc
    24721 ccttataatt gcttatgtcc tgccggacgt tcgcgcgtcc ggcacctctc ttacttaaga
    24781 atcccgaaat ccctgatggc atgattcatc cagtacccgg caccgtcctc aaaattccag
    24841 gtacgccaga ccagggcacc gttattgcgg acaaccaggc gaaaatgttc cccgtggtca
    24901 tcctcgataa agacgttccg gtactgtgcg gcaacggctt ccgcctgttc gcgggtaaag
    24961 gagccgtcag gcagcccctg aagtattctg aaatattcgc tcatcctcat atctccggtg
    25021 atgccccgcg cagggcgggg cggttatcac agtcaggcca gtcagaacgg gatatcgtct
    25081 gaaaacccgt aatcctcccc ctccggcggt tgcggctgag gctcgggctg cgcggcctta
    25141 cgtccacggc ctttcgtttt cgcgccacct tttttcgtcg cgtcagcact ctgtggctgc
    25201 ccattctgct gaggcttcgg ttgcgcctga gcgttctgct gtggtgcact ccccagcatc
    25261 tgcatggtgc ccgtggtctt aacaagaatt tcagtgacgt aacgggtgat accgttatct
    25321 tcccagctac gggtacggag ttgcccctcg atgtagacct gcgcgccctt gcgcagatat
    25381 tcaccagcca cttccgcgag cttgccgaac agcaccacgc ggtgccattc cgtctgctcc
    25441 cgcatctccc ccgtctgttt gtcgcgccag ctttctgacg tagccacctg caggtttgcc
    25501 actgcgcccc cgttggggat gtaacggact tccggatcat tgcccagacg cccgacgagg
    25561 atgaccttgt tgataccacg tgcactcata agaacctcct gactgaaaga tgtgagataa
    25621 accggatacc ggcgacggga cgttcatgtg ccccttcaga ggagcgaaag ctcctcctgc
    25681 cgtccacttc acaggggttt ctccccgcag tggacctttc cgatgggggc ccgtttaccg
    25741 cctcacggga cagctgtccg ggggcggacg caaaattttt tgccgggttc atggctaaaa
    25801 attttgtggc cggtgcatct caccctggac agatggaacg ggagggggtt acgtctgccc
    25861 cccggaaagt ccacagcggg aaacaggccg cagaggtggc ggcaggaggg aagactgctg
    25921 cgccatcagg cgcagtttcc tcacacaccg tcaggtgtgc agggagcgca ggtcacggcg
    25981 cgacaggggc ggcgcgaccg gaacggtcgc tgcaagcccg ggttttcacg catcggaccg
    26041 tagcgacagc ccgaaaccgc gccagcgggt tcggcggaga ctggcgggtg cgcaagcagc
    26101 tgcctgtcgg agtcgggcct gacgaccgga tgccgccagg cagccgggag ccgaagtggg
    26161 cgcaataaaa gacagccaga aacggtgaca gtgtcacgga atatcacacc tgttccctgc
    26221 atacagccat acactacctt ctgaccggaa agccgggccg gcagcgccgc aggcgctgca
    26281 ccccgcattt ccggcaggcg tcagcctgcc agggggtttc gcgcgggctg acaggcggcc
    26341 tccaccgcct gcccctgtat cacctgttta tgctgccctg aaggatgtag tgaccgccca
    26401 ggaatacagc ctgctgattc gccacaaaat cgcagatttc atcaacaatc tgcgcagtgg
    26461 tgagcggttt ctgccccatt tcacgacgcc gcgcattaat cacggctttg taattgcgca
    26521 gaatttcgcg ggtttctgtg ctgatattca tgacatgtgc tccccgtcca gcgcctgtaa
    26581 cacaacgtcc attgcttcgc gttcttccct gacgaatgcg tcaccgtcgc ccccgttctc
    26641 gctcccgacg gcatacaggc gcttcatttc actgcctgcc agcgccatca ctttttccag
    26701 catcaccgcc ggaactgtca cctgacggct ggcgcgcttt tcttccatca gctcacgaac
    26761 aacgtcgtga acaatgctga catccgcacc atgctcaaag gcatcgtcca gtcgctgcat
    26821 gacggtttcc agctcatcgt ctgtcagact ccagtcctgg gctatgtgac gcacatcccc
    26881 acgggtcagc accacggcaa aaacgcattc actgtccggc acgtctgtgt tcagcacttc
    26941 gcggataatt tctttaacag tacagtacat gttctcgtct cctttacagg ggcggcattg
    27001 ccgccccgtc cgttaatcag tccagtgccg cgtaaatcgc gttgcattcg gggtgaaatt
    27061 cgatgtgacg ccacaactgc gcatcgcgca tccggtaaag ctgggtcagt cctgcatccc
    27121 cgctgtcgtg gtacagccac aactggcggt taatcacaag ggaagtcaga atgatgcctg
    27181 cggcatcagc actcacggta cggtcaaacc agtttgcacc gttcaccatg tggaaacggt
    27241 cgccatccgg catcatgtaa ccgccaccgt ccggcaggcg gatgaagtgc cagaagcctc
    27301 cctgatattc cggcatcatt ttttgcgcca gcgcgtacac atacgcctcg gcatacagaa
    27361 aatcgttgcc aaacagatac ggtagaaagg agagacggtc gcactcttca acgttcagag
    27421 tgacaggttt cgcatattgc atgatgtttt cctccgttgt tcacatattc cgcgacaccg
    27481 ggcggtgccg tgcctccata aaagccgggc gataaggcgg ggatgcacag ggcgcaaggg
    27541 aagccgcagc ctgacgcaca cggctggcgg gagttggagg gtgagaccgc gaaatctgag
    27601 cgggtgaacg accggaaaat cacggcagac gggaaggagg gaccgggtga ccgcagcggc
    27661 gaagcgtacc cttgcatccc tgccagcccg gctaagatgg aggcacggca cagcccgcac
    27721 acgacgctga ggccacataa ctgaaaaacc acaaggccat gccctgcatg gccgggtaaa
    27781 gcggagccgg cggcgacttc ctttgtgctc tgtccgggga ccggcaggaa aaaggcgggg
    27841 atttaccccg ccaacgtcat catttccgga tttttgccag ggcatccgcc atgctgcgga
    27901 tttcggcccg ggcttcccgg accacctccc gcagaacacc acggtcaaac cagcggcggg
    27961 gcggttcatc cggtatacgc agcacatcgc aggccagccc actggacagg atatgctcaa
    28021 tacaatcgct gaccaggtac atttccaccg ccgaaaaacg tgctccccgg ttcaggatgg
    28081 catcgagcac cacgggcagg ttttcgtcgt cttcatcgag ccagcagggg tgatcgcttt
    28141 ccttcacaac caggcgaaag cggcgcgtct ggcgggtcag caacgtctga ccggttgtgg
    28201 tcagcgccgc cctgatacgc tgcgcatctt ctgccacccg gcaactgaca ctgcgtaccg
    28261 catcgcgcac tgcatcaaga actgccctga tttcgtcttc ggtcagccat tctccggcat
    28321 acgggaaaaa cacctgtgtt ccgtctgccg gaagtaccgg cagcgactgc cactgactgc
    28381 cagacagtac cagttccgcc ccggttccgg tcagtttctg aatggcggag gccaggagcg
    28441 gcggcagtac gccgccgtga cggttgatga gtaacatcgc cagccggggc gcatccagca
    28501 gaacataaat gctgatccag cccggatact tcgggttccg ctggtcaaca tccgtctctt
    28561 ccggccagcc ctgcgccagc agcaggttgc gggcgtgacg acaaatcagt gcgttcagtg
    28621 ttgtgttttc gttcatgatt aatcgttctc cctgaatcgc cgcccggagg cggcgataac
    28681 atcagtcgtt ttcgtgtcta ttgtttttca gcagggtgcg ggcctgatta atcaccgcct
    28741 gacggcgggt gacgcattcc gggacggcga ggccatgatc ccgggcggcc cattcacgtt
    28801 tccacaatgc ctgttcagcg gtctgaatca gggacgccag cagttcagca gggacggtca
    28861 cctggcggtt ttcgctcacg ttattgatga tctccatcgc cacgccggaa gaaataccgg
    28921 attcagtccg ctggtcttcc gggatgtcct ccaggcgcgc caggacggtt ctgatttcat
    28981 gatcggacag ggaaatatcc attccgtcag cgagggcctg aatctcttcc ggggaccaga
    29041 taaccagcat cagcggcatg tctcccggat actttactgc cagctcccgg catagcgttt
    29101 cgcatgttcc gtacattctc gtctccttct caatctttaa agcacgcttc cagttccaga
    29161 atttcggtat atgcctccat caggaggcgg tttgcgcgaa acagcgtctg acgcaccaga
    29221 cgttccggca gggtgtcggc attaaagctg taccgctgct ccatctcatt catggttgta
    29281 cgcaggtcgc acatggcatt ggccacctcc gccacttcgg cgcgcagttt ttcgtgtcct
    29341 gcatagttca tttacgcagc ctccggcata aaccagtcat cattcgcggc cccctgctgc
    29401 atggcccgtt gcacggcggc aaggcgttgc tgcccggcac ggtgatactg ctcaagcagc
    29461 tcgataccga tataccggcg tccggactgg agggcggcga cgcaggttga gccgctgcct
    29521 gcaaacgggt ccagcacaat tgcgttcggg tgtgtgaagt tctcaatcag cggttgcagg
    29581 ctggtaacgg gcttttccgt cgggtgatgg cgattgcccg aatatttcca gcccagcacg
    29641 tccggcagcg ggttttgtgg cagacgtgga cggccttttg ccaggatgta ggcgcattcg
    29701 tggcgatagc ccacatatgc ggccttcgat gtgtagtttt tggtgaagac caggtgacca
    29761 acaacgctga atcccgcatt tttccaggcg gccataaagc gatcgacgcg gttccagccg
    29821 tagaagctca ccattaacgc gtcttttttc agcacgcgga acatttcatt acaggccggt
    29881 tgcagccact catcggtttt atcgccggcg atggtgcgcc cggaacggtc acggaaaccg
    29941 acgagatatg gcgggtcggt gaggatgaaa tcaatggcgt tatcaggaat acgggccata
    30001 acatcgatgc agttaccgag gacaaaacgg gacatacgaa actccttctc ttacagaggg
    30061 caccgggagg gcacccctgt ggtgcccgtt ccccgctgcc tgtgggctgt tcaccgcgtc
    30121 cggtaacggg tcaggccgac cgtggaatac cgcagccgtc gtctttctga cggcaaggtt
    30181 atgccgcgaa agcggcttga agggcgttca tcagaaaagg gcgacgggct ggcgtacact
    30241 gtcaccgacg ccagccggga gcggtaaaag cgcccgtccc gtgcccgccg gacgcgggac
    30301 actgaccaca ggcagcgttt atgccgcgcc gcgtcagccg gcgcagtaaa aagaggagca
    30361 tggcgacatc ctttaccgga aaagaaacgg gcggttgccc gcccgtctcc gtcatacttc
    30421 atccaccgta atgcgccacc ttgtcgacta tccacgcagg tccggtgact tccggcagct
    30481 catcatcatc tgtcggggaa gaccattcca gctcccccca cagcacatca acaagctctc
    30541 cgcgctcata caactgccag ccacagaaat cacagccctg ttcggcatac cagtgctcca
    30601 gcgtgcagct gaaacggcgg cttaattctg caataacgtc gctttccggc tgacaccacg
    30661 gcgtgtcgaa atcaacctga atgaagttgt ctccctgact actgatattc aggtcatacc
    30721 cacaaggcca cttcacgcca taccgttctg tataccagtg atatgcagaa ggaacaccgt
    30781 tcagaacacc accgttaaag ccattcactt caacatccag gcgggtgggc agaaccgtca
    30841 gcaggtcaaa cggcatggtt ttttccggca gtacattgtc acacagacgg ttccaccaca
    30901 ccgatacatc ctcgttactc cagaagccac accagtcacc tcttttggcg gtgaaatgca
    30961 cggtgatggt ttctctgacc tcatccggta actcttccca gcgacgctgt ccagtaccgc
    31021 tctgcagcca gagttcatgc agcatcaggc agttttgctc atccagcagt acaccgttct
    31081 gcaggtgtgt cagccagcgg gtgaacgcga tattttccgg tgatacagca ccacgtccgg
    31141 cagccgtcac tccggggcac tgctcagagc ggatattttc agtgatctgc agaagtccgg
    31201 cacttccggc cagaaacagc tgaatacctt catttgtggc gcggcgataa agcggtgtga
    31261 ctgcaccgct ggccagtcgt ttaatctcag cgatctgtgc tggttcacca gaaaaataca
    31321 tacgattcga gcaccagttt ggcatgggaa ttctcctctc acatgcggcc tccatcaggc
    31381 cgtgccgcac ctgtaaccgg gcgaccgcat acgaatgcag gtgcggcccc gaagccgcag
    31441 cgcgtggagg aggaacgacg aaacgtgacc gggtgaggag gccggcgaca gcgacacact
    31501 tgcatcggat gcagcccggt taacgtgccg gcacggcctg ggtaaccagg tattttattc
    31561 acagaaccgt gcgcaaaatg ctgtggataa gctggacaca gcagtccaca gcaggcatac
    31621 agccacactc ccagagaatt gagcgttttt tagtcacaat aatgacattt gactgtgtgt
    31681 ggattcagtt attagaatac tgtatatgca tacagcaata gaaaagggag atgagaacat
    31741 gatccgcatt gaaattcttt tcgaccgcca gagcacaaaa aacctcaaat caggcacact
    31801 tcaggcgcta cagaatgaaa tcgaacaacg tctgaaacca cactacccgg aaatctggct
    31861 gcgtatagac cagggcagcg ccccgtctgt atctgtcaca ggagcccgca acgacaagga
    31921 taaagaacgt atcctgtctc tgctggaaga aatctggcag gacgacagct ggctgcctgc
    31981 agcatgagta ccgtttatca ccgtccggct gacccttcag gcgatgacag ttacgtgcga
    32041 ccgttgtttg ccgatcgttg ccaggccggt tttccttcgc ctgccactga ttatgctgag
    32101 caggaactgg atctgaacag ctattgcatc agcagacctg cagccacctt ctttctgcgc
    32161 gccagcggtg aatcgatgaa ccaggctggc gtgcagaatg gtgatctgct ggtagtggac
    32221 agggccgaga aaccacaaca cggggacatc gttatcgctg agatcgacgg tgagttcacc
    32281 gtcaaacgac tgctgttgcg cccacgcccg gcactggagc cggtttcaga cagcccggaa
    32341 ttccgcacac tgtatccgga aaacatctgt atttttggtg ttgtcactca cgtgatacac
    32401 aggacgcggg agttacgctg atgtttgcac tggctgatat caacagtttc tacgcctcat
    32461 gtgaaaaagt tttccgcccg gaccttcgca acgaaccggt catcgtactc agcaataacg
    32521 atggctgtgt gatcgcgcgc agcccggagg caaaagccct tggcatcaga atggggcagc
    32581 cctggtttca ggtgagacag atgcgcctgg agaagaaaat acatgtattt tccagcaatt
    32641 atgcgctgta ccacagcatg agccaacggg tcatggctgt tctggagtcg ctttctcccg
    32701 cagttgagcc ctactcaatt gatgaaatgt tcattgattt gcgggggata aatcattgca
    32761 tctctccgga ggtttttggt catcagctca gggaacaggt aaagagctgg acaggactca
    32821 ccatgggggt gggcattgcg cctacaaaaa cgctggctaa aagtgcacag tgggcaacaa
    32881 agcaatggcc acagttttcc ggagtggtcg cgctgacggc agaaaaccgt aatcggacct
    32941 tgaagctact ggggctgcag ccagttggtg aggtctgggg agtaggacgc agactgacgg
    33001 aaaagctgaa tgcgctgggt attaacacag cactgcagct ggcgcaggct aacacggcat
    33061 tcatccggaa aaacttcagc gtcattcttg agcgtacggt acgcgaactc aacggcgagt
    33121 cctgcatatc cctggaagaa gcaccaccgg caaaacagca gattgtctgt agtcgcagtt
    33181 ttggtgaacg aatcacagac aaagatgcca tgcaccaggc tgttgttcag tatgcagagc
    33241 gggccgcaga gaaactacgt ggggagcgtc agtattgccg gcaggtgacg acatttgtac
    33301 ggacatcccc ctttgcagta aaagaaccct gttacagcaa tgccgctgtg gaaaagctt
//
LOCUS       DBANUU010000043        32993 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000043 DBANUU010000000
VERSION     DBANUU010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.043"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(226..1818)
                     /gene="lsrK"
                     /locus_tag="ACIE68_001951"
     CDS             complement(226..1818)
                     /gene="lsrK"
                     /locus_tag="ACIE68_001951"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFX5908270.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1897..2850)
                     /gene="lsrR"
                     /locus_tag="ACIE68_001952"
     CDS             complement(1897..2850)
                     /gene="lsrR"
                     /locus_tag="ACIE68_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFX5908271.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3099..4634
                     /gene="lsrA"
                     /locus_tag="ACIE68_001953"
     CDS             3099..4634
                     /gene="lsrA"
                     /locus_tag="ACIE68_001953"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFX5908272.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKISAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVEEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMTDRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4628..5656
                     /gene="lsrC"
                     /locus_tag="ACIE68_001954"
     CDS             4628..5656
                     /gene="lsrC"
                     /locus_tag="ACIE68_001954"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFX5908273.1"
                     /translation="MLKFIQNNREITALLAVVLLFALPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5656..6648
                     /gene="lsrD"
                     /locus_tag="ACIE68_001955"
     CDS             5656..6648
                     /gene="lsrD"
                     /locus_tag="ACIE68_001955"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFX5908274.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYIKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNSLP"
     gene            6660..7682
                     /gene="lsrB"
                     /locus_tag="ACIE68_001956"
     CDS             6660..7682
                     /gene="lsrB"
                     /locus_tag="ACIE68_001956"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFX5908275.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7709..8584
                     /gene="lsrF"
                     /locus_tag="ACIE68_001957"
     CDS             7709..8584
                     /gene="lsrF"
                     /locus_tag="ACIE68_001957"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFX5908276.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8608..8898
                     /gene="lsrG"
                     /locus_tag="ACIE68_001958"
     CDS             8608..8898
                     /gene="lsrG"
                     /locus_tag="ACIE68_001958"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFX5908277.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8955..9713
                     /gene="tam"
                     /locus_tag="ACIE68_001959"
     CDS             8955..9713
                     /gene="tam"
                     /locus_tag="ACIE68_001959"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFX5908278.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLQQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9717..10631)
                     /locus_tag="ACIE68_001960"
     CDS             complement(9717..10631)
                     /locus_tag="ACIE68_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFX5908279.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10838..12289)
                     /gene="uxaB"
                     /locus_tag="ACIE68_001961"
     CDS             complement(10838..12289)
                     /gene="uxaB"
                     /locus_tag="ACIE68_001961"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFX5908280.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12516..13934)
                     /locus_tag="ACIE68_001962"
     CDS             complement(12516..13934)
                     /locus_tag="ACIE68_001962"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFX5908281.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRMLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14073..14432)
                     /locus_tag="ACIE68_001963"
     CDS             complement(14073..14432)
                     /locus_tag="ACIE68_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFX5908282.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14432..15358)
                     /gene="glsB"
                     /locus_tag="ACIE68_001964"
     CDS             complement(14432..15358)
                     /gene="glsB"
                     /locus_tag="ACIE68_001964"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFX5908283.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15422..16810)
                     /gene="sad"
                     /locus_tag="ACIE68_001965"
     CDS             complement(15422..16810)
                     /gene="sad"
                     /locus_tag="ACIE68_001965"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFX5908284.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16911..17792
                     /locus_tag="ACIE68_001966"
     CDS             16911..17792
                     /locus_tag="ACIE68_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFX5908285.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17870..18984
                     /gene="yneK"
                     /locus_tag="ACIE68_001967"
                     /pseudo
     CDS             17870..18984
                     /gene="yneK"
                     /locus_tag="ACIE68_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            19134..20324
                     /gene="ydeA"
                     /locus_tag="ACIE68_001968"
     CDS             19134..20324
                     /gene="ydeA"
                     /locus_tag="ACIE68_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFX5908286.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGSGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(20349..21014)
                     /gene="marC"
                     /locus_tag="ACIE68_001969"
     CDS             complement(20349..21014)
                     /gene="marC"
                     /locus_tag="ACIE68_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFX5908287.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            21226..21660
                     /gene="marR"
                     /locus_tag="ACIE68_001970"
     CDS             21226..21660
                     /gene="marR"
                     /locus_tag="ACIE68_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFX5908288.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            21680..22063
                     /gene="marA"
                     /locus_tag="ACIE68_001971"
     CDS             21680..22063
                     /gene="marA"
                     /locus_tag="ACIE68_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFX5908289.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            22095..22313
                     /gene="marB"
                     /locus_tag="ACIE68_001972"
     CDS             22095..22313
                     /gene="marB"
                     /locus_tag="ACIE68_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFX5908290.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(22370..23809)
                     /locus_tag="ACIE68_001973"
     CDS             complement(22370..23809)
                     /locus_tag="ACIE68_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFX5908291.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPCIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(23834..25507)
                     /locus_tag="ACIE68_001974"
     CDS             complement(23834..25507)
                     /locus_tag="ACIE68_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFX5908292.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     FTAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(25563..25874)
                     /locus_tag="ACIE68_001975"
     CDS             complement(25563..25874)
                     /locus_tag="ACIE68_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFX5908293.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(25902..27224)
                     /locus_tag="ACIE68_001976"
     CDS             complement(25902..27224)
                     /locus_tag="ACIE68_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFX5908294.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            complement(27339..27650)
                     /locus_tag="ACIE68_001977"
     CDS             complement(27339..27650)
                     /locus_tag="ACIE68_001977"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFX5908295.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            27849..28547
                     /locus_tag="ACIE68_001978"
     CDS             27849..28547
                     /locus_tag="ACIE68_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFX5908296.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRIIPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(28592..29491)
                     /gene="eamA"
                     /locus_tag="ACIE68_001979"
     CDS             complement(28592..29491)
                     /gene="eamA"
                     /locus_tag="ACIE68_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFX5908297.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            29686..30873
                     /gene="ydeE"
                     /locus_tag="ACIE68_001980"
     CDS             29686..30873
                     /gene="ydeE"
                     /locus_tag="ACIE68_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFX5908298.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVKLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            31000..31095
                     /gene="mgtS"
                     /locus_tag="ACIE68_001981"
     CDS             31000..31095
                     /gene="mgtS"
                     /locus_tag="ACIE68_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFX5908299.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            31095..31199
                     /gene="mgtT"
                     /locus_tag="ACIE68_001982"
     CDS             31095..31199
                     /gene="mgtT"
                     /locus_tag="ACIE68_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFX5908300.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(31314..32204)
                     /gene="dgcZ"
                     /locus_tag="ACIE68_001983"
     CDS             complement(31314..32204)
                     /gene="dgcZ"
                     /locus_tag="ACIE68_001983"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFX5908301.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(32459..32851)
                     /gene="ydeI"
                     /locus_tag="ACIE68_001984"
     CDS             complement(32459..32851)
                     /gene="ydeI"
                     /locus_tag="ACIE68_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFX5908302.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         8656 a         8167 c         7634 g         8536 t
ORIGIN      
        1 accccttttg catccattag tgtctttagg aatattcgct ataaaataag ggttgtgctt
       61 aatgctttaa gaaaaatagc aattttccct tgaatatagt gctggtgatg gaacgatgaa
      121 tctgcaagct ggcttttaac aagccagctc taaaagaagg gaaataagaa taactatact
      181 caaaaactaa cagccacggt catcatgatg tggctgtcaa tgaaactata acccaggcgc
      241 tttccataac gacgtcgtca gtccatgatc aaccagcccc agctgatcct gataaactgc
      301 ctgccactta tcgcgtgaat cctgataaag ttcatgcttt tccgggtctg gtgtgtgcgt
      361 ccgttcccag cgaaccaggc gttctccggt ttctgccatt gatgaaaaaa ttccggcacc
      421 gacgccagct gcaatggcac atcctaatgc agtggcttct ttgaccaccg gaatattgac
      481 gggtaatccc gagacatcag cgagaatttg actccataat ttcccttttg aacctccgcc
      541 tgcaaagact aacgatgaag gatgaatatt cgagaaatca gcaatttgct gcaagttaca
      601 cgctgataca atcgccgcat tttcttccag cgcacggaac aatgtcgctt tgttacattt
      661 atccgggtca atggacaagt taataaagga aggcgcagcg tgataccagg ttttaaagcg
      721 cattctgtcg gagaagatcg gcattacgcc ccacgaccca ggcggcaccc gactggccat
      781 ctcttccagc agcgtatagg tgtcgatgcc taaacgttcg gcaatcagtt tttcttcggc
      841 acagaaagca tcgcggaacc agcgcatggt gagtccggta aaaaagctta tagattcagc
      901 ttgtaccatg ccaggaataa catgaggatt aacgcgcacg ttcatttctg ggtctgtcac
      961 cggcgcggct aaatttacaa cttgctgcca gaatgtgccg ccaagaaccg cggtttgtgc
     1021 cggacgcaca acgcctaacc caaggcaacc aagctgcacg tcgcctcctc caacgaccac
     1081 cggagtgccc gccttcagac cgcagagttc cgccgcttgt gaacttacca cgcccagcaa
     1141 tgtgccggtt tctttgacag gagaaagaat atcggcacgt aggccagcca tatccagcaa
     1201 tgcaggtttc cagtcacggg tggttagatc aagaagtccc gtggtgccag cgttagaggg
     1261 atccaccgcc agttcgccgc tgagcatata ggccagccag tcgctgatca tggtgatggt
     1321 tgatgcctga cggtaaatat cggaacgatg gtgcgccagc caaagtaatc tggggatggc
     1381 acttaaagcc agtgtttgtc cggtcgcgcg ataaacttcg ttttcaaagg tattgttgtg
     1441 cagttcttta agttcgctaa cttcgcgtgc cgctctggca tccacattgg cgcaggccca
     1501 gatcggggct ccttcattat tatataaaac aatgccttca cgcatcgaac atgccgaaac
     1561 ggcagcgata tactccgggg ctatgccggc gttgtgcagc gcctggcgca tacactcaca
     1621 cgccagttgc cagtttttgt tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc
     1681 caggtgccgc cactccgcct gtcccactgc tatttgattg ccttccaggt cgaatatcac
     1741 agcccgaata cttccggtac ctgcatccag cgccatcagg taatactttg attctgaagg
     1801 ggtaaagagt cgagccatta gattatcctc ggcttatgcg ttctgtctgt tcctctatac
     1861 gttctccatc attcccggta ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc
     1921 tgtgtcctga tcggtaacca gtgcgttgat ataaccgcct ttcattgcag cggcaattgc
     1981 ttcggcttta ttttctcccc ctgccacgcc aacccggacg ggtatggtct tcagcgagct
     2041 taaaggtaag ccaatcagtt cgttatgtat tttaatatcc gtgacaacgt caccttttgc
     2101 atcaaaaaag tagcctaaaa tgtcgccaac cgcccctttt cgaccaatca ttaactgttc
     2161 gccctggctg atataaccgg agcgaatgat cgtcgcgtcg tcctgttgac tgacggcccc
     2221 aatgccgaca atcgccacat cggctgcttg cgcggctaac agaacatctt tgacgcaatt
     2281 ttcatttttt agcgtacggg caatgtcagc ggaggatgcc cgcaacggag ccggaataat
     2341 attcacactg cacgccgcgt taagctgccc gatacccgtc atataagaac cgacgccacc
     2401 ggagagcgtg accaggcgaa tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt
     2461 attcatggtt gcctcgccaa aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat
     2521 caacatatgc gccgcgccta tccccagtcg cccgccgaca tcagcatccg caagcccagg
     2581 gatcacccgg acatgttgca gcgaaaactg acgacgtaat tgagtttcat attccagaca
     2641 gccttcaaag cgagaattaa tctgtacgcg aataatgccg gactgatgcc ctttctccag
     2701 caagcgcgac actttcaaac gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag
     2761 cccgtcgtga tagtaaaacc acgcgatccg cgcgacctgt tcttcttcac acattccctg
     2821 ttctgaaatt gccgaatcgt tgattgtcat aattcattct tcactttgaa catatttaaa
     2881 tctttaatgc aattgttcag ttcttgcgca tttatatctg tgatggcagc cacagtttga
     2941 ctctacgagc atgaacaaac gcaaccgtga aaatcaaaat agcataaatt gtgatctatt
     3001 cgtcggaaat atgtgcaatg tccacctaag gttatgaaca aattaaaagc agaaatacat
     3061 ttgttcaaaa ctcacctgca aaactgaacg ggggaaatat gcaaacgagt gatacccgcg
     3121 cgttaccgct actttgcgcc cgctcggttt ataaacagta ttcaggggtc aatgtcctga
     3181 aaggcatcga ttttacgttg catcaggggg aggtccacgc cctgctcggc ggcaatggtg
     3241 ccggtaaatc gacgttaatg aagattagtg ccggtattac ccctgctgat agcggtacgc
     3301 tggagattgg gggcaacaac tacgccagat taacgccagt tcatgctcat cagctgggca
     3361 tttatctcgt tccccaggaa ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc
     3421 tgtttgggat ggcaaaaaaa cagctctcca tgcaaaaaat gaagaacttg ctggcggcgc
     3481 tgggctgcca gtttgatctg catagtctgg caggatcgct ggatgtcgcc gatcgccaaa
     3541 tggtggaaat cctccgcggg ctgatgcgcg actcgcggat tctgatcctc gatgaaccta
     3601 ccgcctcgct tacccctgcg gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg
     3661 ctactggcgt gggtattgtt tttatctcgc ataagctgcc ggaaattcgc cagattgccg
     3721 atcgaattag cgtgatgcgc gacggaacca tcgccttaag cggcaaaacc agcgaactgt
     3781 ctaccgacga cattattcag gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca
     3841 gccaaaaatt atggctggag ttacctggta accgcccaca acatgctgcc ggaacgccgg
     3901 tgctgacact ggaaaatctg accggcgaag gtttcaggaa tgtcagcctg acgctcaatg
     3961 ccggagaaat tctgggcctg gctgggctgg tgggagccgg acgcacagaa ctggccgaga
     4021 cgctctatgg tctgcgtact ttgcgtggcg gacgcattat gctgaatggt aaagagatca
     4081 ataaattatc cactggagaa cgtttactgc gcggtctggt ttatctgccg gaagatcgcc
     4141 agtcatccgg actgaatctc gatgcttcgc tggcctggaa cgtctgcgcc cttactcata
     4201 accttcgtgg attctgggcg aaaaccgcga aagataatgc caccctggaa cgttatcgtc
     4261 gggcgctgaa tattaaattt aaccaaccgg aacaagctgc acggacatta tccggtggca
     4321 accagcaaaa aatcctcatt gccaaatgcc tggaagcttc gccgcaagta ttgattgtcg
     4381 aagagccgac gcgcggcgtg gatgtctcgg cacgtaatga tatctaccag ctgttgcgca
     4441 gcatcgccgc acagaatgtg gctgtgctgc ttatctcctc tgatctggaa gagatcgaac
     4501 tgatgacaga tcgtgtgtat gtgatgcatc agggcgaaat tacccactct gcactgaccg
     4561 ggcgcgatat taatgtcgag actattatgc gcgttgcctt cggcgatagt cagcgtcagg
     4621 aggcatcatg ctgaagttta ttcagaacaa ccgtgaaatc acggcactgc tggcggtggt
     4681 gctgctgttt gcattacccg gttttctcga ccgccagtat ttaagtgtgc aaacgctgac
     4741 catggtttat agcagcgcgc aaatcctgat cctgctggca atgggcgcga cgctggtaat
     4801 gcttacgcgc aatattgatg tttcagtggg ttcgattacc ggaatgtgcg cagtgctgtt
     4861 ggggatgtta ctgaacgcgg ggtattcact acctgttgct tgtgtcgcga ctttactgct
     4921 tggtttgctc gcgggatttt tcaacggtgt cctggtcgcg tggctaaaaa tccctgccat
     4981 tgttgccacc cttggcacgt tagggttgta cagaggcatc atgttgctgt ggactggcgg
     5041 taaatggatt gaagggttac ccgccgaact gaaacagctc tccgcaccgc tgctgtttgg
     5101 cgtttccgcg attggttggt tgacgataat tctggtggca tttatggcct ggctgctggc
     5161 aaagacggcg tttggacgca gtttttatgc cacgggcgat aatttacagg gcgctcgtca
     5221 actgggtgtt cgtacggaag ccattcgcat tgtggcattt tcgttgaacg gctgcatggc
     5281 ggcactggcg ggaattgtgt ttgcttcgca gattggtttt atccccaacc agaccggtac
     5341 cgggctggag atgaaagcaa ttgcagcctg cgtgctgggc ggcattagtt tgctcggcgg
     5401 ttccggtgcg atcatcggtg cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt
     5461 actggtgctg ttgcgcattc cggcatggtg gaacgatttt atcgcgggcc tcgtgctgct
     5521 ggcggtgctg gtgtttgatg gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca
     5581 aaaatatgcc cgctttatga cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa
     5641 acgggaggcc gcataatgcg tattcgctac ggttgggaac tggctcttgc cgcactgctc
     5701 gttattgaga ttgtcgcatt tggtgcaatt aacccgcgaa tgttagatct caatatgttg
     5761 ctgttcagca ccagtgactt tatctgcatt ggcattgtcg ccctaccgct gacgatggtg
     5821 attgtcagtg gcgggatcga tatttcgttt ggttcgacca tcggcctctg cgccattgca
     5881 ttgggcgtac tgtttcaaag tggtgtgccg atgccgctgg cgattctcct gaccttactg
     5941 ctcggcgcat tgtgcgggct gatcaacgcc ggattaatta tctatatcaa agttaacccg
     6001 ctggtgatta cgcttggcac gctgtatctg tttgccggaa gcgccctact gctttccggg
     6061 atggccggag cgacggggta cgaaggtatt ggtggattcc cgatggcgtt tacagatttc
     6121 gctaacctgg atgtgctggg actccccgtt ccgctgatta tcttcctgat atgtctcctc
     6181 gttttctggc tctggctgca taaaacccat gccggacgta atgtgttttt gattgggcaa
     6241 agcccgcgcg tggcgcttta tagcgcgatt ccagttaacc gcaccttatg tgcgctctat
     6301 gccatgacgg ggctggcgtc tgcggtcgcc gctgtgctgc tggtgtcgta ttttggttca
     6361 gcacgttccg atctcggtgc gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc
     6421 ggtgccaata tttatggtgg ttccggttcc attatcggca ccgccattgc ggttttatta
     6481 gtgggatatt tgcaacaagg tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc
     6541 ctttccggtg cgctacttat cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag
     6601 caaattaaag agtggctggc gcgtcgggcc aataactcat tgccataaag gatatcttca
     6661 tgacacttca tcgctttaag aaaatcgcct tacttagcgc tcttggcatt gccgcaatct
     6721 ctatgaatgt gcaggccgca gagcgtattg catttattcc caaactggtt ggcgtgggat
     6781 tttttaccag cggtggcaac ggcgcacaac aagcgggtaa agagctgggc gttgatgtga
     6841 cctacgacgg gccgacagaa cccagtgttt ctggtcaggt acagttgatt aataacttcg
     6901 tcaatcaagg ttataacgcc attatcgttt ctgcggtttc gcctgatggc ttgtgtccgg
     6961 cactgaaacg cgccatgcaa cgtggtgtga gagtgctgac ctgggactct gatactaaac
     7021 cggagtgccg ctcttactac attaatcagg gaacgcccgc ccagttggga ggtatgttgg
     7081 tggatatggc ggcgcgtcag gtgaataaag acaaagccaa agtcgcgttt ttctactcaa
     7141 gccccaccgt tacggaccaa aaccagtggg tgaaagaagc gaaagcgaaa atcgccaaag
     7201 agcatccagg ctgggaaatt gtcactacgc agtttggcta taacgatgcc actaaatcgt
     7261 tacaaacagc agaaggaata ttaaaagcgt atagcgatct cgacgccatt atcgcccccg
     7321 atgccaacgc cctgcccgct gccgcacaag ccgcagaaaa cctgaaaaat gacaaagtag
     7381 cgattgtcgg attcagtacg ccaaatgtga tgcgcccgta tgtagagcgc ggcacggtga
     7441 aagaatttgg cctgtgggat gtggttcagc aaggcaaaat ttcagtgtat gtcgcggatg
     7501 cattattgaa aaaaggatca atgaaaacgg gcgacaagct ggatatccag ggagtaggtc
     7561 aggtggaagt ctcgccaaac agcgttcagg gctatgacta cgaagcggat ggtaatggca
     7621 tcgtactgtt accggagcgc gtgatattca acaaagagaa tatcggcaaa tacgatttct
     7681 gatgtgcatt acttaaccgg agtaagttat ggcagattta gacgatatta aagatggtaa
     7741 agattttcgt accgatcaac cgcaacaaaa tatccctttt accctgaaag gttgcggtgc
     7801 gctggattgg ggaatgcagt cacgcttatc gcggatattt aatccgaaaa cgggtaaaac
     7861 cgtgatgctg gcttttgacc atggttattt tcagggaccg actaccggac ttgaacgcat
     7921 tgatataaat atcgccccgc tgtttgaaca tgccgatgta ttaatgtgta cgcgcggcat
     7981 tttgcgcagc gtagttcccc cggcgaccaa taagccggtg gtactgcggg cgtcaggtgc
     8041 gaactctatt ctggcggaat taagtaatga agccgtggcg ttatcgatgg atgatgccgt
     8101 gcgcctgaac agttgcgcgg tggcggcgca ggtttatatc ggcagcgaat atgaacatca
     8161 gtcgatcaaa aatattattc agctggtcga tgccggaatg aaagtgggaa tgccgaccat
     8221 ggccgtgact ggcgtgggca aagatatggt gcgcgatcag cgttatttct cgctcgcgac
     8281 tcgaatcgcc gctgaaatgg gggcgcaaat tatcaaaacc tattatgtcg aaaaaggttt
     8341 tgaacggatt gttgccggat gtccggtacc cattgttatt gctggcggta aaaaattacc
     8401 ggagcgcgag gcgctggaaa tgtgctggca ggctatcgat cagggcgctt ctggtgtgga
     8461 tatggggcgt aatattttcc agtctgacca tccggtggcg atgatgaaag ccgtacaggc
     8521 ggtggttcac cataacgaaa cggctgatcg ggcatatgaa ctctatctga gtgaaaaaca
     8581 gtaactgcgg atttaaggag aagaattatg cacgtcacac tggttgaaat taacgttcat
     8641 gaagacaagg ttgacgagtt tatcgaagtt tttcgccaga accacctggg ctcggtacag
     8701 gaagaaggca atttgcgctt cgatgtctta caggacccgg aagtgaattc gcgcttttat
     8761 atctacgaag cctataaaga tgaagacgca gtggcattcc ataaaaccac gccccactac
     8821 aaaacctgtg tcgcgaaact ggaatcttta atgaccgggc cgcgtaaaaa acgtctgttc
     8881 aatgggttga tgccgtgagg cgaatttatc aattttatct acaattgggg taacgcgctg
     8941 acgggagtaa aaaaatgtct gactggaacc cctctttata tctgcacttt gccgctgaac
     9001 gatcgcgtcc ggcggtggag ctgcttgcca gagtgccgct ggagaatgtc gaatatgttg
     9061 ccgatcttgg ctgtggccca ggtaacagca ccgcccttct acaacaacgt tggcctgctg
     9121 ccaggataac gggcatcgac tcgtctccgg cgatgattgc tgaagcgcgc agtgctttgc
     9181 cagactgcca gttcgtggaa gcagacatcc gcaactggca accggtacag gcactcgatc
     9241 tgatttttgc taatgcctca ctgcaatggc tgcccgacca ctacgaattg tttcctcatc
     9301 tggtttcttt acttaatccg cagggcgtgc tggcagtaca gatgccagat aactggctgg
     9361 agccgaccca tgtgctcatg cgcgaagttg cctgggaaca aaactacccg gatcgtgggc
     9421 gtgagccgtt ggctggcgtt catgcttact acgatatttt gagcgaagcc ggatgtgagg
     9481 tcgatatctg gcgaaccacc tactatcacc agatgccgtc acaccaggcg attatcgatt
     9541 gggtgaccgc cactggatta cgtccgtggt tacaggatct gaccgagagc gaacagcagc
     9601 tttttcttaa gcgctaccat cagatgctgg aagagcagta tccactgcaa gagaacggac
     9661 agatactgct ggcatttccg cgtcttttta ttgttgcccg ccgtacggag taaatatcac
     9721 gtcagctggt aatgacgatc gggaagaatt tttgctggaa tttcggcttc atcgttcatc
     9781 tgtaacaggt caatttcaat agcgttgcag atggcatcca gcggtaaatc attgttttca
     9841 gtaccgaacg gatcttccag ttcttccgcc agacaatcca gcgaaataaa agtgtaggaa
     9901 atcagcacag agataaaagg cgtcatgtaa tgcaggtcca cgaccagcgc gaacggcagc
     9961 atgatacaaa acagataaac ggtacgatgc aaaatcaggg tgtaggcaaa gggaattggc
    10021 gtataggcaa tgcgctcaca gcctgccaga accgctgaaa tatcattaag ccgatcgttg
    10081 aggctaataa acaggatatc tgaaagctgt ccattgcggc gctgaaccgc caaccattct
    10141 cccattatta acaagatacg gttagctgga gagttcgaag ccagtacacg ctgaagatct
    10201 tcagtcttga gataatgagc cagcacttcc gcctgtggct gtttgcgtaa tgtcatgcgt
    10261 aaacagtggg cgaaggcgat ttgcagccgg gcaaactccc ttacacttgc cgaatccggc
    10321 aatgtcgttt ttacctcgcg cagtaacgac cgtgaggcaa tcatcaactg cccccaaagt
    10381 tttcgcgctt caacgtaacg ggcgtacccg gcattattac gaaaaccaag aaaaatggcg
    10441 atggcgacac cgagaatgct gaacggtgcg agggtgaatt tgatgcccag atgcgtgtac
    10501 cagggcagca tgaatataac agcgatagaa aagagaaaat tgagtagtaa gcgcgaggat
    10561 atcttggata atactgagcc gtgccagaca aaaatacggc gcagccagtg ttgttgtgga
    10621 cgaacaatca tggttatctt caggcgtgga aaagtcgccc tattaaacgt gattacgatc
    10681 acattctcaa gacgctactt acaaattacc tacaagctta caacattaac aggcgctgca
    10741 tgtggcacct gttaatgata aaggcacata ggatgttgta actaatatag tgaaataaga
    10801 aacccggtcg aaaccgggtt cagaagtaac ggtgttatta gcacaacgga cgtacagctt
    10861 cgcgcatccc tttttcaaga atcgcatcca ggtcattagc aacctgctcg accagaccag
    10921 gcacttgcgt caggtcctgc tcccagtggt ctttttctgc caaaacaatc gctaccagtt
    10981 cctgggtgcc gataacacgg tcacgatgct ggctccagag ttgctgataa cgttccagcc
    11041 agtgtgcatc gtcctgtacc ggatatgttt ctccgttacg ctcaccgcga tagaaggcaa
    11101 tcaatgccgc taatgcgaaa gtaaggcgcg ccggaagtgt gccttttgcc ttctgccctg
    11161 ccagcagctg cggcaggatg cgggtgcgga acttggtcat accgttgagc gcgatagaca
    11221 gcagctgatg cttaatgtac gggttacgga aacgcccggt gactgcactg gcgaaagatt
    11281 ccaattcatc acgaggcaaa tccagtaccg gaataatttc ttcgtagata gctttttcaa
    11341 cgaatgcgca aatttcagca tcgttcatcg cttcacctac ggtatccagc cctgcctgga
    11401 acgccaccgg caccagcgcg gtgtgagcac cgttgaggat tgccactttg cgctctttat
    11461 acggtttaat atcgtcaaca atcagcacgt tgagtggata tttgtccagg cgcagctcag
    11521 tcgctaagga tttcggcccc tgaatcacaa acaggtaaaa gtgttcagcg gtgtcgagga
    11581 aaccatcgtg ataacccagc tcttcttcca gtttagccac ttcatcgcgc ggataaccgg
    11641 taacgatacg gtccaccagc gtagaacaga agctgttagc ctgatccagc cattgaataa
    11701 atgcttctgg cagtgcccac tcttgcgcat agcgcagcac cagttcacgc aacgcgtcgc
    11761 cattgtagtc gatcaactca cacgggatga tgatccaacc tttatccagc gcaccgttga
    11821 aatggctaaa acgttcgaac agcagacggg tcagttttgc cggataactt accgctggcg
    11881 cgtcatcgaa tttatcgccc gcatggtagc tgatacccgc ttcggtggta ttagagaaaa
    11941 caaagcgcat ttccgggttg tgagccagtt tcaggaattc atcgtattca ctgtagacgc
    12001 tgatttcacg attaaccgag cgaatcagac gcgcgtcgct gaccgcttcc cccttctcat
    12061 tcaggccacg gataatggtg gtatacagac catcctgcgt gctcagtgac ggtgggaatg
    12121 aagtttcaat cggacgaaca acgaccacac cagaattcag atcggtgtgc tcattcagga
    12181 gatcgatttg ccagtcaaca aaggcgcgca ggaagttacc ttcaccaaac tgaatgatac
    12241 gttctggata ctgtgcaccg ggaaaatcgc gacgatttag tgttttcaca atgggttccc
    12301 ttctgattag tcatacaacc tgtttgaatt ggtacgacag gttagcaaac tttaatacgc
    12361 cgaacccctg ttttgatcaa ctcctgatga ttaatgagca gttttatgag aaaagtgtgg
    12421 cgcggatcat ggtttaatcg aggaaatacg ccttttcctg gatcataaag tggtagaaca
    12481 cattgcattc aaatcgcgcg gaatgaataa agatgtcaga caacttcctc accgtaacgc
    12541 atagtgctgg tacggttgcg cccatctttc ttcgagcgat acagacacgt atcggcttcg
    12601 accatcaatt tattaaaatc atcggtcagc attcggtacg atgcgcgacc actaccgacg
    12661 ccaatactta ccgtgagata aagcgttttt tgttgccagg tgaatggttg cagttcaacg
    12721 cctttacgga ttttttccgc cattagcaga ccatcgacag gattcaccga cggcactgca
    12781 acagcaaatt cttcgccgcc catgcgcgcc accagcccct tatcaccgac aatcttctga
    12841 atatgccggg caaacacgct taacacttta tcgccacatt catgtccata gttatcgtta
    12901 atgcttttga aatagtcgat atcaagcagc atgaccgtca gatgttgtgt ctgtttcagc
    12961 gatggacttt tcagcgcctc ataaagaccg gaccgtgagt aaacctgagt cagaaaatca
    13021 aagtcggctc gcagcgcaac ttgcttcatt agcgaattga tcgctgccac gctaaaagaa
    13081 accataattg ggcatatcgc catcgtggca ataccgagac gtgcggagaa catttccgga
    13141 atggagaacg gcgaaccgac cgagatatca atcaccgaat ttgccaccag tacgatttcc
    13201 accgcaccgg tgacaaaggt caacagacat gttacctgcg gcgtatagcg cactgcacac
    13261 cagattaatg caggcagcgg gaacgccaga ctccccgctc cgccaatgac taccgaggca
    13321 ataaccgaca caataagcgc gatggcgggc atcatctgtt ctgctttaaa gcgcggcagc
    13381 actccaggaa tagccaacgt cagcatacaa ggcacgatca acacgcccgt tgagaattgc
    13441 tcactgaacc agtccgcaag caaaggccag aaatccagac tatcaatact gaccgaacca
    13501 atcgccccga caatagcgca taataatgcg gcaatcagac agtaattaaa tagccgtaag
    13561 gcacttactg gctcatactt atttttcccc agacgcttat cccgcacgac cagtaaggcg
    13621 acggtaacaa taaacatcat attggagaaa ttgataacca gtgaaaccag tccccattcg
    13681 gtggttatgg catcataaac cagcatcgcc acataactga tcgcatagta atgcagtcga
    13741 ttaagccaga catagcgggc aaacaccccc gccattacgc cattcagcgg ccagaaaaga
    13801 gaaagttcgt gaaccagccg taactctgca ccaataaaat agaacagcgt ggttagcgca
    13861 aaaatagcga tgctattacg tagcagatga ccttcctgga acaaacggaa agttgaaatg
    13921 gggtgtacat gcattaatta tctgtccata caattgcggg tggcaattat ctcaacgtaa
    13981 taagtaaatt ctcataaagg atagcatgtt cgctaacagc ggcggcaaat aaaggtaccc
    14041 tgggaatgct attttatcgt tttccgcaat tctcatggct gattcatctg tacaaccagc
    14101 cagtgataga ttaccgccac gatataacgt tgttgttctt cgcttaacga aacaccctgc
    14161 ggaatgttat gccacttagc cagacaaccg cgacagcagg tcgcggtggc gtgctgagcg
    14221 ataaacaccg gatgcccgcg catgggggtt tgcttaccgt cattagccgg taatgccgga
    14281 gcaagacgtt tagcgacaaa atccgccgca tgttgttcga tgactggcgc gcctttctcc
    14341 aggcaatact gacgctcttt catgcccaga cgaaagcgag agcgaaattt tgaacgggat
    14401 aaacgcgcga agagtggatc gagagactgc attaataaac cgaacgccct aactgtttcg
    14461 tcaattgttc aaggacggca atacccgcaa gcgagttacc tgcatcatcc agttccggac
    14521 tccagacagc gatggccatt tcatgcggaa caatcgccac aataccgcca ccaacgccag
    14581 atttcgccgg tagccccacc cgccaggcaa actcccccgc gttctggtac ataccactgg
    14641 tcgccatcag cgcgttaatt tgccgcgcct gcattggcgt caccacaggt tcatcaatat
    14701 gaatagcttt cccctgatta gccagaaaga caaacgtcct ggccagctct acacagctca
    14761 ttttcagagc gcagtaatga aagtagtttt gcagaacggt tgtcacatca tgatggaaat
    14821 tgccaaacga cttcatcagc caggcgatag ccgcatttcg cgcggaatgt tcaaattcgg
    14881 aacgcgctac caccgtatcg tatgaaatat cagacacacc gcttaagccg cgcacgactt
    14941 ccagcatacg ttgccgtggt gcgcttaatc gcccttgcaa catatcgcaa accaccagcg
    15001 caccagcatt aataaacgga ttacgcggaa taccctgctc catttccagt tgcactaaag
    15061 aattgaacgg tgatccagac ggatctttgc cgacgcgttg ccagatttcc tcttcggagt
    15121 aatgacgcat ggcgacaacg agactcagca ctttggaaat agactgaatg gaaaaacgtt
    15181 cttgcgcgtc tccggcctga aaaagctgtc cgtcaacggt acagatagca atgcccaatc
    15241 gggaaccgtc tactgtagcc agcgccggaa tataatccgc gactttaccc tgaccaatga
    15301 gcggccgcac ttgccgcaag atgttttcta aaattgcatt atccatggcg actgccactt
    15361 tctactcctg gaccgcaggt ctgaaaagac ctgcgagtat atcagagctg aatatgtcgc
    15421 gtcagatccg gtctttccac accgtctgga tattacagaa ttcgtgtaag ccgaaatggg
    15481 aaagctcacg accaaagcca ctctttttca cgccaccaaa ggccactcgc gcgtcgctgg
    15541 cacaataacc attgataaac accccaccgc attccagacg tgccgccatc tgtctggcct
    15601 gtgtttcgtc ggtggtaaaa atggtcgctg aaaggccgaa ctcactatca ttagccagtt
    15661 ccagcgcatg ttctgcatct ttcgcaacgg tgattgccgc aacagggcca aacatttctt
    15721 cccgaaacgc ggtcatttct ggggtaacat tcgccagaac cgttggcgga tagtagttac
    15781 ctgccccagc catcttttcc ccgcccagta acaaacgcgc accctgcgcc agggttttct
    15841 ccacctgatg atgcagctca tcacgtaaat caaaacgagc cattggtccg agagcgttct
    15901 cttcatcacg gggatcgccc attttcaagg ctgccgcagc tgccacaaaa cgttcggtaa
    15961 atgcagaagc aattccctct tcgataataa agcgttttgc cgctgcacaa acctgtcctg
    16021 tattctgata acgtccggct accgccgctt taaccgccag ttccagatcg gcatcgttaa
    16081 gcacaataaa tggatccgaa ccgcccagtt ccagtacgca ttttttcagt gccgctccag
    16141 cctgtgcgcc aatagccgct cccgcacgaa cacttccggt caccgtgaca gcagcaatgc
    16201 gcgaatcttt aatcatttga ctgacaccgt cgttgtcggc attcagccag ccatatacgc
    16261 cttgcgggat tcccgcatct ttaaacacct gggcaatgag ctgagcacag cccatcacat
    16321 tcggcgcatg tttaagtaag tagccgttac ctgcaagaat gatgggaacc gcgccacgca
    16381 tcacctgcca taacggaaag ttccacggca taatcgccag aatcgtcccc aacggtcgat
    16441 actcaataac tgcctgctga ttttccacca gcgtaggttc cgccttcagc attgccggac
    16501 catgttctgc ataccagtca cacaaattcg ccgatttcgc cacttcagcg cgcgcctgat
    16561 tgattggttt gcccatttca cgggtgatca tttgcgccat ttcttcgcta cgggcgcgca
    16621 gagccttacc gataccacgc agtttttcag cacgataatc tatatttgtc tcgcgccagt
    16681 cgcgaaagcc tgctgccgcc agctgaagtg cgttttcgat atcgttagcg ccagcccacg
    16741 gcagcacaga aagttgttca cccgtggcag gatttatcga aattgcatga gttgccggag
    16801 taatggtcat cggggtatct cctttatgag tcatggtatg aagatacgca gatttactct
    16861 tgctttaaaa tgaataatat taagccactt attcacgaat cgagaatatt atggatctga
    16921 cccaactgga gatgttcaac gcggttgccg aagctggcag cataacccag gcagcagcaa
    16981 tagtgcatcg cgtgccgtcg aatctcacta cccgtctacg ccagctggaa acagaactgg
    17041 gggtagatct gtttattcgc gagaatcagc gtttacgtct ctctcctgcc gggcataact
    17101 ttttacgcta tagccaacaa attctcgcgt tagtggatga agcgcggagc gttgtcgctg
    17161 gcgatgagcc gcaaggttta ttttctcttg gttcgctgga aagcaccgca gcagtgcgca
    17221 ttccggccac gctggcggaa ttcaaccatc gttatcccaa aattcagttt tcactttcca
    17281 ccggcccttc cggcactatg ctggaaggag tgctggaggg aaaactgaat gcggcattta
    17341 ttgatgggcc cattaaccat actgccattg acgggatacc ggtataccgc gaggagctga
    17401 tgatcgtcac gccacaaggt catgcgccag taatccgcgc cagtcaggtt aacggcagca
    17461 acatttatgc cttccgcgcc aattgttcgt atcgtcgcca cttcgagagc tggtttcatg
    17521 ctgacggtgc cgctccggga actattcacg agatggagtc gtatcacggg atgttggcct
    17581 gtgtggtcgc aggagcaggc attgcgctta ttccacgctc tatgctggaa agtatgccgg
    17641 ggcatcacca ggttgaagcg tggccgttag ctgagcaatg gcgttggtta acaacctggc
    17701 tggtctggcg tcgtggtgcg aaaacgcgtc cgcttgaggc atttattcaa ctactggatg
    17761 tgcctgactc ggcaaaacag ggttaccaat gagctatttt tgatagttct tgcagctcaa
    17821 atgccctata tagtgatgtt ccgatgactt atgactatat ggggcaaata tggttacgcc
    17881 agtaagcatc agtaattaca tatctcttcc tgatgatttt cccgtacgta atattgcacc
    17941 tcaggtaaaa gaagttttaa aggattttat tgatgcactc agtaccataa tatgtgatga
    18001 agagtggcgc acgtctttaa acaccaactc agccacaaaa aagatattta acaaccttga
    18061 taacttatca tatattcaga ggacttcttt tcggggtaac gacacactat acaatgaaaa
    18121 ggttcaattt aaactcactt attccgtcaa gaatggaaga cacaaagaaa atattgaatt
    18181 tcaggtagta ataaatttaa gccctattta tttagataat tttcgccatg atggagaaat
    18241 taatattttt gcgctcccca cccaaagcct gtcactatgg ggcgcgtatt tcagaccggc
    18301 gtcgagcgtg tgctatttct gtttctgaat gattttattg aacaatttcc aatgatcaac
    18361 cttggcgttc ccatcaaaag agcgcataca ccacatattg aacccctgcc tccggatcgc
    18421 cataccgctg cagattactt acgccagttt gatttgcttg tcctgaattt tatctctcgc
    18481 ggtaattttg tcatactccc ccgattatgg agtaactctg aggttcacag atggttcgtc
    18541 aataaagatc ctaacttgat caccgccatt ctcgacataa cagacggcga attaaaagag
    18601 gatttattgc aaagcctgat ggattcatta ggttctaaca aacatgtaca acccgaagtc
    18661 tgcatctgct ttttatccct tttagcagaa caagaatctc ctcattttca ggactttttt
    18721 ttatttttcg ccaatatgtt attgcactat caccaattta tgaatcccaa tgaaagtgat
    18781 ctgaatgacg tgttgatgcc agcatcatta agtgatgata aaattatcaa acatatggcg
    18841 cgcaggaccc tcaaactgtt tgtaaaaaat gaaacgccgc caaaagttac tcacgaagac
    18901 ctggtgaaaa acaggcctcg ctcccctgtc agaccaccta tacccgcaac cgccaaaacg
    18961 ccagacctcc ctgaacgtca ttaaaccgtg atgttaccga ctctctgacg cggaacagaa
    19021 tcagcgtcag agaaacggaa aacgcgatcc agatcacaaa tgcattgtat tcacatcatt
    19081 aaccgtttta agatcatttc atcacttttt cgcaactcac ccgataatct gttatgacaa
    19141 caaacactgt ttcccgcaaa gtggcgtggc tacgggtcgt tacgctggca gtcgccgcct
    19201 tcatcttcaa caccaccgaa tttgtccctg ttggcctgct ctctgacatt gcgcaaagtt
    19261 ttcacatgca aaccgctcag gtcggcatca tgttgaccat ttacgcatgg gtagtagcgc
    19321 taatgtcatt gccttttatg ttaatgacca gtcaggttga acggcgcaaa ttactgatct
    19381 gcctgtttgt ggtgtttatt gccagccacg tactgtcgtt tttgtcgtgg agctttaccg
    19441 ttctggtgat cagtcgcatt ggtgtggctt ttgcacatgc gattttctgg tcgattacgg
    19501 catctctggc gatccgtatg gctccggccg ggaagcgagc acaggcattg agtttaattg
    19561 ccaccggtac agcactggcg atggtcttag gtttacctct cgggcgcatt gtgggccagt
    19621 atttcggttg gcgaatgacc ttcttcgcga ttggtattgg ggcgcttatc acccttttgt
    19681 gcctgattaa gttacttccc ttactgccca gtgagcattc cggttcactg aaaagcctcc
    19741 cgctattgtt ccgccgcccg gcattgatga gcatttattt gttaactgtg gtggttgtca
    19801 ccgcccatta cacggcatac agctatatcg agccttttgt acaaaacatt gcgggattca
    19861 gcgccaactt tgccacggca ttactgttat tactcggtgg ttcgggcatt attggcagcg
    19921 tgattttcgg taaactgggt aatcagtatg cgtctgcgtt ggtgagtacg gcgattgcgc
    19981 tgttgctggt gtgcctggca ttgctgttac ctgcggcgaa cagtgaaata cacctcgggg
    20041 tgctgagtat tttctggggg atcgcgatga tgatcatcgg gcttggtatg caggttaaag
    20101 tgctggcgct ggcaccagat gctaccgacg tcgcgatggc gctattctcc ggcatattta
    20161 atattggaat cggggcgggt gcgttggtag gtaatcaggt gagtttgcac tggtcaatgt
    20221 cgatgattgg ttatgtgggc gcggtgcctg cttttgccgc gttaatttgg tcaatcatta
    20281 tatttcgccg ctggccagtg acactcgaag aacagacgca atagttgaaa ggcccattcg
    20341 ggcctttttt aatggtacgt tttaatgatt tccaggatgc cgttaataat aaactgcaca
    20401 cccatacata ccagcaggaa tcccatcaga cgggagatcg cttcaatgcc acccttgccc
    20461 accagccgca taattgcgcc ggagctgcgt aggcttcccc acaaaataac cgccaccagg
    20521 aaaaagatca gcggcggcgc aaccatcagt acccaatcag cgaaggttga actctgacgc
    20581 actgtggacg ccgagctaat aatcatcgct atggttcccg gaccagcggt acttggcatt
    20641 gctagcggca caaaggcaat attggcactg ggttcatctt ccagctcttc cgacttgctt
    20701 ttcgcctccg gtgaatcaat cgctttctgt tgcggaaaga gcatccgaaa accgataaac
    20761 gcgacgatta agccgcctgc aattcgcaga ccgggaatcg aaatgccaaa tgtatccatc
    20821 accagttgcc cggcgtaata cgccaccatc atgatggcaa atacgtacac cgaggccatc
    20881 aacgactgac gattacgttc ggcactgttc atgttgcctg ccaggccaag aaataacgcg
    20941 acagttgtta atgggttagc taacggcagt aacaccacca gccccaggcc aattgcttta
    21001 aacaaatcta acattggtgg ttgttatcct gtgtatctgg gttatcagcg aaaagtataa
    21061 ggggtaaaca aggataaagt gtcactcttt agctaacctt gcatcgcatt gaccaaaact
    21121 tgaaccgatt tagcaaaacg tggcatcggt caattcattt atttgactta tacttgcctg
    21181 ggcaatatta tcccctgcaa ctaattactt gccagggcaa ctaatgtgaa aagtaccagc
    21241 gatctgttca atgaaattat tccattgggt cgcttaatcc atatggttaa tcagaagaaa
    21301 gatcgtctgc ttaacgagta tctgtctccg ctggatatta ccgcggcaca gtttaaggtg
    21361 ctctgctcta tccgctgcgc ggcgtgtatt actccggttg aactgaaaaa agtgttgtct
    21421 gtcgaccttg gagcactgac ccgtatgctg gatcgcctgg tctgtaaagg ctgggttgaa
    21481 aggttgccga acccgaatga taagcgcggc gtactggtaa aacttaccac cagcggcgcg
    21541 gcaatatgcg aacaatgcca tcaattagtt ggccaggacc tgcatcaaga attaacaaaa
    21601 aacctgacgg cggacgaagt ggcaacactt gagcatttgc ttaagaaagt cctgccgtaa
    21661 acaaaaaaga ggtatgacga tgtccagacg caatactgac gctattacca ttcatagcat
    21721 tttggactgg attgaggaca acctggaatc gccactgtca ctggagaaag tgtcagagcg
    21781 ttcaggttac tctaaatggc acctgcaacg gatgtttaaa aaagaaaccg gtcattcatt
    21841 aggccaatac atccgcagcc gtaagatgac ggaaatcgcg caaaagctga aggaaagtaa
    21901 cgagccgata ctctatctgg cagaacgata tggcttcgag tcgcaacaaa ctctgacccg
    21961 aaccttcaaa aattactttg atgttccgcc gcataaatac cggatgacca atatgcaggg
    22021 cgaatcgcgc tttttacatc cattaaatca ttacaacagc tagttgataa cgtgacaacg
    22081 tcactgaggc aatcatgaaa ccactttcat ccgcaatagc agctgcgctt attctctttt
    22141 ccgcgcaggg cgttgcggaa caaaccacgc agccagttgt tacttcttgt gccaatgtcg
    22201 tggttgttcc cccatcgcag gaacaaccac cgtttgattt aaatcacatg ggtactggca
    22261 gtgataagtc ggatgcgctc ggcgtgccct attataatca acacgctatg tagttattgt
    22321 caggctctga tcacagagcc tgacatctcc gtaatatctt cctcaagtct caaatatttt
    22381 cgccattgtt ggcgatgaga tcccgatacc agtaaaacga cgctttacgt atccgttcca
    22441 gcgttccctt cccttcgttg tctttatcga cataaatcat tccgtagcgc tttttcattt
    22501 cccctgttcc ggcagaaacc aggtcaatgc agccccacgg ggtgtagcca attaagtcaa
    22561 caccatcttc aactacggct tttttcattt cccgaatatg ggaagcaaag taatcaatgc
    22621 gatagtgatc gttcaccgtg ccgtcagctt gtctctggtc aaccgcacca aatccatttt
    22681 cgacaataaa cagcggcaac tggaaatgat cccagaacca gttgagtgaa taacgtagcc
    22741 ctgccggatc aatttgccat ccccattcgg atgtatcgat acaagggttg ctgaccaaat
    22801 ctcgtggttc aacataatcc agttgcgggt tatcgggcga aaattgtgtc gtaaaagaca
    22861 tgtagtagct aaagccgata aagtcgacac aaccactggc aagaatcgcg ttatcttctg
    22921 gtgtgatatc gaggttgaat cctttcctgg caaagtaatt cagcatatgt tgcggataat
    22981 atccacgagc atgaacatca gtaaaccagt aacgacgatg cattgctttc gtggccatca
    23041 tcatatcgtt gggtgcacac gtcagaggat agatgggaca catggcgatc atacagccaa
    23101 tgttaaattc aggattgatc gattttccag tctgtaccgc cagggcactg gcaactaact
    23161 cgtaatgtac cgcctggtac attatttgtt cgcgctcatt gatatcttcc tctggcgaat
    23221 acaagatacc ggaattagta aatggacaca ggctttcgct gaaattcacc tgattattga
    23281 tttcgttaaa cgtcatccag tactttactt tttctttata gcgcgtgaag accgttgatg
    23341 cgaagcggat aaaaaagtcg atcagtttac ggtttcgcca gccaccatat tttgtcacca
    23401 gatgataagg catctcaaaa tgtgaaagcg tcaccacagg ttccattccc tgcttaaggc
    23461 attcatcgaa cagatcatca taaaattgta aaccctcttc attcggctcc tgttcgtcac
    23521 cttgcggaaa gattcgtgtc caggcaatgg aagttcgaaa gcatttgaat cccatctcgg
    23581 caaataactg aatatctgtt ttatagcgat gataaaaatc aattgcttca tgattgggat
    23641 aattaagccc gtcgataacg ccttctgtca cttcacgcgg caccccgtga gcgccagcag
    23701 tcatcacatc agcgatgctg atgccttttc ctccttcatt ccagccacct tccaactgat
    23761 gcgcggctac cgcaccgccc cataaaaaac cttttttaaa tcctgacata cacaactcct
    23821 taactaaaat agattaccac cagacttccg cctggatgcc ggccataaac tcgtctttgc
    23881 tgttctcatt aaacttaaaa ttcgataatt cgttatccag aatattgata tacgttccat
    23941 agaagcgaat ttctggtcgt gaacctaaaa tgctttcacc cactttcaat gcatgccaga
    24001 gtgtcgtttt ataagccgat tcatttagtg ttacaccctg ctgagttttg ttctgttgct
    24061 taaaccagcc taattcaagc cccgtctgat tccatgtatt ccagatccag gccggtctta
    24121 ttacagtgcg aatactgtca aaatcactat gagcaccact ttcataacta taaacatctt
    24181 cgccgtgaga atagacaagc gcgttagcca taataatatt gtcagtaaga tacatctcgc
    24241 cctgagagat taaacgccag gcgatcccat tggtatgatc accatagtaa tagcgaccat
    24301 gcgccatcgt attactggca tctgagaaac tggcaaaact gctggcatag gaattattgg
    24361 caacctgtaa cgtaaattca ttaaacgtat cgcgttgcag gttttgccgt aaaacagaag
    24421 tcagcatcca gctatctttc atttcaaaat aactgttgtc attttcgtta tcttgttgtt
    24481 gatccgtttt attaggtgcg gaatatttag ccattaatga taatgtcgca tcatcccata
    24541 acgggatatt gcgataacga acatccacag aattggtatt catctggctg gtacgcgaaa
    24601 aatcacgaga gtaaacatcg acatcatcac ggcttaagga catatcaaac agccctacac
    24661 caagtgccca gttttcaatc cccacacccg cggcgacatc cgtggttaag gttttccagt
    24721 ccagcatctg gatctcatat tgcgggagtt tatgtttgcc gacccagaag tctgcctctg
    24781 gtgcgaaggg taaaaaacct cgcgttgtca ggtaaatatc gctgaactgc atgatatttt
    24841 cattggcact gtcaccaaac caggcatcgt tatactgctc acccacgttg ccatcatagg
    24901 taacgaccgc atttgccgtt ttaccgtcct gattataaac acgctgattt aaggtcaggt
    24961 caaaccaacc gctcatctca ttaccaaaac gtcccagaga acccgctgca taagtttgtg
    25021 gtgagccgtg attaccagta ccccagcctg agcggaagta cccctgatag ctgaacccaa
    25081 tatcatcctt tatatattta ctgatatctt tcagggtcac gctttcgata gtggttttgt
    25141 cgcctttact ttcaataacg gcaacttgct gcgatccatt tatagtatgc gctgcggtaa
    25201 acgagttctg ttcaccgttt tcatttttga tgttatcagc gacgggagat gtcgctgata
    25261 tttcagcaag cgaggctgat ttatatttgt tttccgtgat gcttttttgt aaggtcgaaa
    25321 gtcgggattt atatactccc agttcattct gtgttgcttt cagctcttgt ttattttgcg
    25381 acaattcatt ttcaagcaat tcaaggcgct gctctatggt taattttgtt gccatcgcgt
    25441 tcaatggaaa caatgctgta ataatagaaa aagacaaaag gctgacgtta agcgtcttta
    25501 tattcatgac ttatccttaa gagtataata atttcctcca gctatcgcac tggaggaaaa
    25561 aatcaggcat ggagttcttt tcttaatgaa ataatttccc tggctaaatc ctggcataat
    25621 attgcattca tcaaatgatc ctgtgcatga atcagaatta aattaacttt tattttgcca
    25681 cagccttcat cctgactaat taatgcggtc tgaatcttat gtgcctgaag acaggcagat
    25741 tcagaactgg ccattagctg agttgcgccc tgccagtctt tttgcccggc acaacgtatg
    25801 gcttccatcg cgtcagaacg tgcctgcccc gcgttgacca gtaactccat aacaagttct
    25861 tcatctgcaa acatcacaat ctccttcctg ggtaaatgga attatccgtt agttgtcatg
    25921 ttattgactt tgttggcggc aataacaaaa ggtaaataga tcattgtcgc cacacctaaa
    25981 cataaaaaac caaccagtaa agccagccag ttccccccgg ttccgaggaa agcaattaaa
    26041 ggtccgggcg tagtccaggg cacggcataa gcaaccggtg gaataatttc catcgaaaca
    26101 aagaagtaac caatggcaca gttaacaatg ggaaccataa taaacggcac catcatgatg
    26161 gggttaagta caataggaag gccaaacatt atcggttcat tgatattgaa aataccgggg
    26221 ataaatgaca tttttgccag atcacggtat tccgcacgct tagaagcgat aaaaatagcc
    26281 aacaataacc ccaacgtcat acctgaaccg ccgcagttgg cgaatgcatc attaatagaa
    26341 gtccaggtaa tcggatatgg cgcgccccag gtagtgccat gttgagcggc ccaggagaga
    26401 ttctccagat tagcctcaga aaaaacggtt tctcgaattg ccgatgtggt attaggtccg
    26461 tggatcccca gcacccacag gaaattaccc acagccccca ggataattac cgcaaagata
    26521 ttcgttccca tatgtttgag cggcgtctgg attaatgtgt aaataagatc gtttagtcct
    26581 gccggggata tcagtgtcag gcaataatta agtgcggaaa agaacaccat gacaaaaaat
    26641 attggcaata aaactttaaa tgaacgcgct accgcaggcg gtacagctgc cggcatcgtt
    26701 atggtaattt ttgggtttcg agctaagcga caaaatattt cactggtgat caatgcaacg
    26761 ataacagcaa caaacaaccc ctgcgcgccg agatatttgg tcgtcaggaa tgcttgccca
    26821 tctatcatct gatatggcgt ataaacaata aaaaatgctc ctattgccgt taatccgcat
    26881 aaaagatcat cttgctcata gctaatcgcc atattcctgg cgaccaggaa agcaatcata
    26941 attgacatga tatttacaga accattcatt accggagtaa aaatagcttg tgctttttca
    27001 aggttgggga aaatgctatt cagatgcagt aatccggcaa taaaaccgtc tggcgataat
    27061 atggcaaagt taattaatat aattaatgaa cttgccatga taattggaaa cgtaaaaacg
    27121 aatccatctc tcaccgctgc aacatgtttt tgtgagttta acttgatagc aactggaaca
    27181 agaaaacgtt ccattccacg ttcgaatgag gccattaatc ccattttttt atacctgttg
    27241 ttttggatat ttatacatcc caataaagca tgtatgccat atccatttta tgatgtgtga
    27301 aaagttagtt caggtaatag tttaataaaa ttagattctt actcttgcag tagtttcatc
    27361 gcaaattcaa gaactgcctg tccgttcatg gtgccgtagt ctttcatctc gataacggct
    27421 acaggcattt tcccctgtgc aacggcttca atctctggtt tctgaaagcg aacttgcggg
    27481 ccaagtaaaa caacgtcaac ttcgttattt ttaattttcc ctttagcctc tgcaatcgcc
    27541 aatgcggaaa tattaacttc aagtgaaata gcgttagcat gatcaatcat acgtttaacc
    27601 agcatactgg ttgacatgcc cgcagcgcaa actaacaata tttttttcat attttccctt
    27661 actggtatat aacagattac attgtttaaa tatcatatcc cgcagtagct atacgtcctg
    27721 gtacattgat cacagtaata cagtgataaa gttgttcaac atcagggtga ttattaaacg
    27781 actcggccat acgataaacc aatcaggact caactccttg ttttcaatgg ctcttaaaag
    27841 gtgcaaaagt gatttttcaa aagattgccc gcttacttaa atccgaaatc aatggcaatt
    27901 catggcatgt tggtgatttg ttgccgtcag aagcggaact cgctgttcgt tataatgttt
    27961 cgcgtaatac cctgcgtaag gcattatcct tgctggaagg cgaaggaatt attcacagaa
    28021 agcatggttc aggaacatac attcaaaaaa agaattttgt tgcacacatt gatcacatga
    28081 acagtttcag tgaaattgcg cataaaagcg gcaaagaggc agggagtcag attatgaaat
    28141 ttgaagtgca ggatgcttca cccactattg cgactgagct gaatttagtg actggtgagc
    28201 aggtttatta cataaagaga ctgcgattca ttgaggataa tgcagcacaa ttagaagaaa
    28261 cgtggatgtc agtagcacgt tttcctgatt taaccgtgtc acatatgcaa aaatccaaat
    28321 tttcatatat tgagaatgaa tgcgggatca aaatcattgg cacctttgaa actttctccc
    28381 ctacttttcc taccccagaa atcgccagta ttttacggat catcccacgg gatcccatac
    28441 ttaaaattca gacccaggct gtggacagta actctattcc gctggattat tcgttacttt
    28501 acagcaatat tttcgagttc caggtaaagt actttttccc gcgataaaag cagacgcaca
    28561 gagtgctgac atcacattct gtgcgtatgg tttagcctct aacctttacc gctttacgcc
    28621 accgcaagcc aaatacattg atatacagcc cggtcataat gagcaccgca cctaaaaatt
    28681 gcagacccgt taagcgttca tccaacaata gtgccgcact tgccagtccg actacgggca
    28741 ccagtaacga taacggtgca acccgccagg tttcatagcg tcccagtaac gtcccccaga
    28801 tcccataacc aacaattgtc gccacaaacg ccagatacat cagcgacaag atggtggtca
    28861 tatcaatagt aaccagactg tgaatcatgg atgcggaacc atcgagaatc agcgaggcaa
    28921 caaagaaggg aatgattggg attaaagcgc tccagattac cagcgacatc accgccggac
    28981 gcgttgagtg cgacatgatc tttttattga agatgttgcc acacgcccaa ctaaatgctg
    29041 cagccagggt caacataaag ccgagcatcg ccacatgctg accgttcaga ctatcttcga
    29101 ttaacaccaa tacgccaaaa atcgctaagg cgatccccgc caattgtttg ccatgcaatc
    29161 gctccccgaa agtaaacgcg ccaagcacga tagtaaaaaa cgcctgtgcc tgtaacacca
    29221 gcgaagccag tccagcaggc ataccgaagt taatggcaca aaaaagaaaa gcaaactgcg
    29281 caaaactgat ggttaatcca taccccagca gcaaattcag tggtactttc ggtcgtgcga
    29341 caaaaaagat agccggaaaa gcgaccagca taaagcgcaa accggccagc atcagcggtg
    29401 gcatgttatg aagccccact ttgatgacca caaaatttag cccccatacg accactacca
    29461 atagcgccaa caccccatct tttcgcgaca ttctaccgcc tctgaatttc atcttttgta
    29521 agcaatcaac ttagctgaat ttacttttct ttaacagttg attcgttagt cgccggttac
    29581 gacggcatta atgcgcaaat aagtcgctat acttcggatt tttgccatgc tatttcttta
    29641 catctctaaa acaaaacata acgaaacgca ctgccggaca gacaaatgaa cttatcccta
    29701 cgacgctcta ccagcgccct tcttgcctcg tcgttgttat taaccatcgg acgcggcgct
    29761 acgctgccat ttatgaccat ttacttgagt cgccagtaca gcctgagtgt cgatctaatc
    29821 ggttatgcga tgacaattgc gctcactatt ggcgtcgttt ttagcctcgg ttttggtatc
    29881 ctggcggata agttcgacaa gaaacgctat atgttactgg caattaccgc cttcgccagc
    29941 ggttttattg ccattccttt agtgaataac gtaaagctgg tggtgctctt ttttgccctc
    30001 attaactgcg cctattctgt ttttgctacc gtgctgaaag cctggtttgc cgacaatctt
    30061 tcgtccacca gcaaaacgaa aatcttctca atcaactaca ccatgctaaa cattggctgg
    30121 accatcggtc cgccgctcgg cacgctgttg gtgatgcaga gcatcaatct gcccttctgg
    30181 ctggcagcta tctgttccgc gtttcccatg cttttcattc aaatttgggt aaagcgcagc
    30241 gagaaaatca tcgccacgga aacaggcagt gtctggtcgc cgaaagtttt attacaagat
    30301 aaagcactgt tgtggtttac ctgctctggt tttctggctt cttttgtaag cggcgcattt
    30361 gcttcatgca tttcacaata tgtgatggtg attgctgatg gggattttgc cgaaaaggtg
    30421 gtcgcggttg ttcttccggt gaatgctgcc atggtggtta cgttgcaata ttccgtgggc
    30481 cgccgactta acccggctaa catccgcgcg ctgatgacag caggcaccct atgtttcgtc
    30541 atcggtctgg tcggttttat tttttccggc aacagcctgc tattgtgggg tatgtcagct
    30601 gcggtattta ctgtcggtga aatcatttat gcgccgggcg agtatatgtt gattgaccat
    30661 attgcgccgc caggaatgaa agccagctat ttttccgccc agtctttagg ctggcttggt
    30721 gccgcgatta acccattagt gagtggcgta gtgctaacca gcctgccgcc ttcctcgctg
    30781 tttgtcatct tagcgttggt gatcattgct gcgtgggtgc tgatgttaaa agggattcgt
    30841 gcaagaccgt gggggcagcc cgcgctttgt tgatttaagt cgaacacaat aaaaatttaa
    30901 ttcagccttc gtttaggtta cctctgctaa tatctttctc attgagatga aaattaaggt
    30961 aagcgaggaa acacaccaca ccataaacgg aggcaaataa tgctgggtaa tatgaatgtt
    31021 tttatggccg tactgggaat aattttattt tctggttttc tggccgcgta tttcagccac
    31081 aaatgggatg actaatgaac ggagataatc cctcacctaa ccggcccctt gttacagttg
    31141 tgtacaaggg gcctgatttt tatgacggcg aaaaaaaacc gccagtaaac cggcggtgaa
    31201 tgcttgcatg gatagatttg tgttttgctt ttacgctaac aggcattttc ctgcactgat
    31261 aacggatcgt tgacacagta gcatcagttt tctcaatgaa tgttaaacgg agcttaaact
    31321 cggttaatca cattttgttc atcaataaac atgcagcgat ttcttccggt ttgcttaccc
    31381 tcatacattg cccggtctgc tcttccaatg accacatcca gaggctcttc aggaaatgcg
    31441 cgactgacac ctgctgtcac ggtaatgttg atatgccctt cagaatgcgt gatggcatgg
    31501 ttatcgacta actggcaaat tctgacacct gcacgacatg cttcttcatc attagccgct
    31561 ttgacaataa tgataaattc ttcgcccccg tagcgataaa ccgtttcgta atcacgcgtc
    31621 caactggcta agtaagttgc cagggtgcgt aatactacat cgccgattaa atgcccgtag
    31681 gtatcattaa ccaatttaaa tcggtcaata tccaacaaca ttaaataaag attcagaggc
    31741 tcagtgttgc gtaactgatg atcaaaggat tcatcaagaa cccgacgccc cggcaatccc
    31801 gtcaaaacat ccatattgct acggatcgtc agcaaataaa ttttgtaatc ggttaatgcc
    31861 gcagtaaaag aaagcaaccc ctcctgaaag gcgtcgaaat gcgcgtcctg ccagtgattt
    31921 tcaacaatag ccagcattaa ttcccgacca cagttatgca tatgttgatg ggcagaatcc
    31981 attagccgaa cgtaaggtaa ttcatcgtta tcgagtggcc ccagatgatc aatccaccga
    32041 ccaaactggc acagtccata agaatggtta tccgttattt ctggcttact ggcatctctc
    32101 gcgaccacgc tgtgaaacat actcaccagc cactggtagt gggcatcgat agccttattg
    32161 agatttaaca agatggcatc aatttccgtt gtcttcttga tcattgccac tcctttttca
    32221 cagttccttg tgcgcgctat tctaacgaga gaaaagcaaa attacgtcaa tattttcata
    32281 gaaatccgaa gttatgagtc atctctgaga taacattgtg atttaaaaca aaatcagcgg
    32341 ataaaaaagt gtttaattct gtaaatcacc tctgcattat cgtaaataaa aggatgacaa
    32401 atagcataac ccaataccct aatggcccag tagttcaggc catcaggcta atttattttt
    32461 atttctgcaa atgagtgacc cgaacgacgg ccggcgcgct tttcttatcc agactgccgc
    32521 taatgttgat catctggtcc ggctgaactt ctcgtccatc aaagacggcc gcaggaataa
    32581 cgacattaat ttcaccgctc ttatcgcgaa aaacgtaacg gtcctctcct ttgtgagaaa
    32641 tcaaattacc gcgtagtgaa accgaagcgc catcgtgcat gttttttgcg aaatcaacgg
    32701 tcattttttt tgcatcatcg gttccgcgat agccatcttc tattgcatga ggcggcggtg
    32761 gcgctgcatc ctgttttaaa ccgccctggt catctgccaa cgcataaggc atgacaagaa
    32821 aacttgctaa tacaatggcc tgaaatttca tactaactcc ttaattgcgt ttggtttgac
    32881 ttattaagtc tggttgctat ttttataatt gccaaataag aatactgcca attgttataa
    32941 ggcatttaaa atcagcccac tagctgtcaa atattcagag aatttaactc act
//
LOCUS       DBANUU010000044        32316 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000044 DBANUU010000000
VERSION     DBANUU010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.044"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(67..1827)
                     /gene="ycaO"
                     /locus_tag="ACIE68_001985"
     CDS             complement(67..1827)
                     /gene="ycaO"
                     /locus_tag="ACIE68_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFX5908303.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1957..2649
                     /locus_tag="ACIE68_001986"
     CDS             1957..2649
                     /locus_tag="ACIE68_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFX5908304.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2848..3936
                     /gene="serC"
                     /locus_tag="ACIE68_001987"
     CDS             2848..3936
                     /gene="serC"
                     /locus_tag="ACIE68_001987"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFX5908305.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4007..5290
                     /gene="aroA"
                     /locus_tag="ACIE68_001988"
     CDS             4007..5290
                     /gene="aroA"
                     /locus_tag="ACIE68_001988"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFX5908306.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5459..6223
                     /gene="ycaL"
                     /locus_tag="ACIE68_001989"
     CDS             5459..6223
                     /gene="ycaL"
                     /locus_tag="ACIE68_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFX5908307.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6396..7079
                     /gene="cmk"
                     /locus_tag="ACIE68_001990"
     CDS             6396..7079
                     /gene="cmk"
                     /locus_tag="ACIE68_001990"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFX5908308.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7190..8863
                     /gene="rpsA"
                     /locus_tag="ACIE68_001991"
     CDS             7190..8863
                     /gene="rpsA"
                     /locus_tag="ACIE68_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFX5908309.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9023..9307
                     /gene="ihfB"
                     /locus_tag="ACIE68_001992"
     CDS             9023..9307
                     /gene="ihfB"
                     /locus_tag="ACIE68_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFX5908310.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9514..11778
                     /locus_tag="ACIE68_001993"
                     /pseudo
     CDS             9514..11778
                     /locus_tag="ACIE68_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            11815..13563
                     /gene="msbA"
                     /locus_tag="ACIE68_001994"
     CDS             11815..13563
                     /gene="msbA"
                     /locus_tag="ACIE68_001994"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFX5908311.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13560..14546
                     /gene="lpxK"
                     /locus_tag="ACIE68_001995"
     CDS             13560..14546
                     /gene="lpxK"
                     /locus_tag="ACIE68_001995"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFX5908312.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            14583..15815
                     /gene="ycaQ"
                     /locus_tag="ACIE68_001996"
     CDS             14583..15815
                     /gene="ycaQ"
                     /locus_tag="ACIE68_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFX5908313.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            15867..16049
                     /gene="ycaR"
                     /locus_tag="ACIE68_001997"
     CDS             15867..16049
                     /gene="ycaR"
                     /locus_tag="ACIE68_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFX5908314.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16046..16792
                     /gene="kdsB"
                     /locus_tag="ACIE68_001998"
     CDS             16046..16792
                     /gene="kdsB"
                     /locus_tag="ACIE68_001998"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFX5908315.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIRNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            16946..17839
                     /locus_tag="ACIE68_001999"
     CDS             16946..17839
                     /locus_tag="ACIE68_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFX5908316.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17816..18595)
                     /gene="elyC"
                     /locus_tag="ACIE68_002000"
     CDS             complement(17816..18595)
                     /gene="elyC"
                     /locus_tag="ACIE68_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFX5908317.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            18731..19516
                     /gene="cmoM"
                     /locus_tag="ACIE68_002001"
     CDS             18731..19516
                     /gene="cmoM"
                     /locus_tag="ACIE68_002001"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFX5908318.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDHVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19513..20835
                     /gene="mukF"
                     /locus_tag="ACIE68_002002"
     CDS             19513..20835
                     /gene="mukF"
                     /locus_tag="ACIE68_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFX5908319.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20816..21520
                     /gene="mukE"
                     /locus_tag="ACIE68_002003"
     CDS             20816..21520
                     /gene="mukE"
                     /locus_tag="ACIE68_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFX5908320.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21520..25980
                     /gene="mukB"
                     /locus_tag="ACIE68_002004"
     CDS             21520..25980
                     /gene="mukB"
                     /locus_tag="ACIE68_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFX5908321.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTSDSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26241..28088
                     /gene="ldtD"
                     /locus_tag="ACIE68_002005"
     CDS             26241..28088
                     /gene="ldtD"
                     /locus_tag="ACIE68_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFX5908322.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28269..28817
                     /locus_tag="ACIE68_002006"
     CDS             28269..28817
                     /locus_tag="ACIE68_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFX5908323.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28844..29491
                     /gene="gloC"
                     /locus_tag="ACIE68_002007"
     CDS             28844..29491
                     /gene="gloC"
                     /locus_tag="ACIE68_002007"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFX5908324.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29713..30903)
                     /gene="aspC"
                     /locus_tag="ACIE68_002008"
     CDS             complement(29713..30903)
                     /gene="aspC"
                     /locus_tag="ACIE68_002008"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFX5908325.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSASLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(31069..32175)
                     /gene="ompF"
                     /locus_tag="ACIE68_002009"
     CDS             complement(31069..32175)
                     /gene="ompF"
                     /locus_tag="ACIE68_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFX5908326.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVSSSNSTPLC"
BASE COUNT         7847 a         7860 c         8706 g         7903 t
ORIGIN      
        1 taaaaaaacg ccccgtaata taactcagac taatcattaa gcctacattg cgtaggctat
       61 ttgattttat tttgcccaga atgctgcttt ggcgcgctgc agcttttcgt aggccttcaa
      121 caacgactga tgtgcagcaa acgcgtgcag atcgctatct accggttgca ggccgtaaaa
      181 cgccgcttcg ccgctcattg ccgcactggc ggcttctacg gcatctgcgc cgtacatgcg
      241 aacaaacgca ttcagatatt gcagcggctg gcgatcttct tcctgtgcca gtaataacaa
      301 cgtttgcagg cagcgataat agttggcgcg ttccggacta aatactgatg agttaaactc
      361 catcgtccat tcggtccaga ccagagcctg ttccagatcg ccaccagcca gcgccagcat
      421 ggcttttaat tcaccgatac gcagggtgta ccaaccgtta tccgacccgg tcgccagacc
      481 caacagctca cgcacgcggg taaagtcatc aaaaccttct tcatccagtt gctcgatgag
      541 gttcaggtaa tcttcttttt cccactcgct gcctggtagc gaaagaatcg tttcacgtaa
      601 atggctgccc atactgttat tcgcgagcca cagatcttca gccggataaa tatcggacat
      661 gccaggcacg ataatgcggc aagcgtagac acccagatgc tcgtaatcgg caatataaac
      721 ttctttatct tcctttttga agatagccat cagcgtggcg aactcttctt ccgtggtgcc
      781 ggagaaattc cagtccacaa acggataatc ggcatcctgc ttgaacaggt cccaggagat
      841 taaaccgctg gaatcgataa agtgcgtttc gaggttggta tgttcagcga cttcttcatc
      901 atcgaaggtt ggcggagtaa acacatccaa atctttcagg ccacgacctt gcagcagctc
      961 ggtcacggta cgttccagtg ctacgccaaa atcaggatgc gcaccgaaag aggcaaagca
     1021 ggtaccgtta gcaggattga acagtaccac gcaaatcacc ggatactggc cgccaagcga
     1081 accgtcataa gcgaagattg ggaaaccctc cgcttccagc gtttcgatgg cttcaactac
     1141 tgctgggtaa cgcgccagca cgtctgccgg gatctctggc aggctgatgc tttcagcaat
     1201 aatgcggttt ttcacgtagc gttcgaaaac ttcagacaat ccctgaacgc gtgcttcgtt
     1261 gcgggtatta cctgcggaca taccgttgga gacgtacagg ttaccaatga tattcatcgg
     1321 aatataaacg gtctgattgt cggactgacg cgtaaacggc aggccgcaaa taccacgatc
     1381 ttcgttaccg gattgtaggt caatcagcat gctgccggtc agttcattct ccggatcata
     1441 aaacgcgcgc agacggtcat cgagtagtcc ttctggcaca tcgtcatttt cggtcagtgg
     1501 gaaccatttt tcgttgggat aatgcacgaa cggaccgttg gcgatggttt cgcccagcca
     1561 gaaatcagca aaaaagtagt tggttgagag acgctcgaaa tattcaccaa gtgcagaagc
     1621 cagcgccgct tttttggttg cgcctttacc gttggtaaaa cacagtgcgc actctttgtc
     1681 gcgaatatgt acagaccaga cgttaggcac aggattcagc caggaggcct cttcaatctg
     1741 aaagccgagg tctgaaagtt tttgctggaa gcgagcgatg gaatcttcca gagcggcatc
     1801 tttgccgggg ataaatgttt gcgtcatgaa aatcacttta gtcgtacgga aagcgcgcaa
     1861 taatacgggt tttatctcaa aggcgctatc accgccgcca tgccgatgaa ctgttgacta
     1921 tgcttttagc ggataaacca cgataagagc ataaaaatga aggcgttcga tctccaccgt
     1981 atggcatttg ataaagtgcc ttttgatttc cttggcgaag ttgcactacg tagtctttat
     2041 acctttgtac tggttttttt gttcctcaaa atgaccggaa gacgcggtgt gcgacagatg
     2101 tcgctgtttg aagtgttaat cattctcacg ctgggttcag cggcgggaga tgtggcgttt
     2161 tatgatgatg tgccgatggt gccggtactt atcgtcttta tcactctggc gttgttatac
     2221 cgcctggtaa tgtggttgat ggcgcacagt gaaaaactgg aagatttact ggaaggtaag
     2281 ccggttgtta ttattgaaga tggcgagctg gcctggtcga aactcaataa ctccaacatg
     2341 acggaatttg agttctttat ggagctacga ttgcgtggcg tggagcagct ggggcaggta
     2401 cgtctggcga ttctcgaaac caacgggcaa atcagtgtct atttctttga agatgacaag
     2461 gtgaaaccgg gtttacttat tttacccagt gattgtacgc agcgttacaa agtggtgccg
     2521 gagtcggcgg actatgcctg tattcgttgt agtgaaatca ttcatatgaa agcgggggaa
     2581 aaacaattat gtccgcgctg tgcaaatcca gaatggacga aggcaagtcg ggcaaaacgg
     2641 gtgacctgac agtaaaaaca tcggcttttt gctaataatc cgagagattc ttttgtgtga
     2701 tgcaagccac atttttgccc tcaacggttt tactcattgc gatgtgtgtc actgaatgat
     2761 aaaaccgata gccacaggaa taatgtatta cctgtggtcg caatcgattg accgcgggtt
     2821 aatagcaacg caacgtggtg aggggaaatg gctcaaatct tcaattttag ttctggtccg
     2881 gcaatgctac cggcagaggt gcttaaacag gctcaacagg aactgcgcga ctggaacggt
     2941 cttggtacgt cggtgatgga agtgagtcac cgtggcaaag agttcattca ggttgcagag
     3001 gaagccgaga aggattttcg cgatcttctt aatgtcccct ccaactacaa ggtattattc
     3061 tgccatggcg gtggtcgcgg tcagtttgct gcggtaccgc tgaatattct cggtgataaa
     3121 accaccgcag attatgttga tgccggttac tgggcggcaa gtgccattaa agaagcgaaa
     3181 aaatactgca cgcctaatgt ctttgacgcc aaagtgactg ttgatggtct gcgcgcggtt
     3241 aagccaatgc gtgaatggca actctctgat aatgctgctt atatgcatta ctgcccgaat
     3301 gaaaccatcg acggtatcgc catcgacgaa acgccagact tcggcaaaga tgtggtggtc
     3361 gccgccgact tctcttcaac cattctttcc cgtccgattg acgtcagccg ttatggcgtg
     3421 atttacgctg gcgcgcagaa aaatatcggc ccggctggcc tgacaatcgt catcgttcgt
     3481 gaagatttgc tgggcaaagc gaatatcgcg tgtccgtcga ttctggatta ttccatcctc
     3541 aacgataacg actccatgtt taacacgccg ccgacatttg cctggtatct atctggtctg
     3601 gtctttaaat ggctgaaagc gaacggcggt gtagctgaaa tggataaaat caatcagcaa
     3661 aaagcagaac tgctatatgg ggtgattgat aacagcgatt tctaccgcaa tgacgtggcg
     3721 aaagctaacc gttcgcggat gaacgtgccg ttccagttgg cggacagtgc gcttgacaaa
     3781 ttgttccttg aagagtcttt tgctgctggc cttcatgcac tgaaaggtca ccgtgtggtc
     3841 ggcggaatgc gcgcttctat ttataacgcc atgccgctgg aaggcgttaa agcgctgaca
     3901 gacttcatgg ttgagttcga acgccgtcac ggttaatgcc gaaattttgc ttaatcccca
     3961 cagccagcct gtggggtttt tatttctgtt gtagagagtt gagttcatgg aatccctgac
     4021 gttacaaccc atcgctcgtg tcgatggcac tattaatctg cccggttcca agagcgtttc
     4081 taaccgcgct ttattgctgg cggcattagc acacggcaaa acagtattaa ccaatctgct
     4141 ggatagcgat gacgtgcgcc atatgctgaa tgcattaaca gcgttagggg taagctatac
     4201 gctttcagcc gatcgtacgc gttgcgaaat tatcggtaac ggcggtccat tacacgcaga
     4261 aggtgccctg gagttgttcc tcggtaacgc cggaacggca atgcgtccgc tggcggcagc
     4321 tctttgtctg ggtagcaatg atattgtgct gaccggtgag ccgcgtatga aagaacgccc
     4381 gattggtcat ctggtggatg cgctgcgcct gggcggggcg aagatcactt acctggaaca
     4441 agaaaattat ccgccgttgc gtttacaggg cggctttact ggcggcaacg ttgacgttga
     4501 tggctccgtt tccagccaat tcctcaccgc actgttaatg actgcgcctc ttgcgccgga
     4561 agatacggtg attcgtatta aaggcgatct ggtttctaaa ccttatatcg acatcacact
     4621 caatctgatg aagacgtttg gtgttgaaat tgaaaatcag cactatcaac aatttgtcgt
     4681 aaaaggcggg cagtcttatc agtctccggg tacttatttg gtcgaaggcg atgcatcttc
     4741 ggcttcttac tttctggcag cagcagcaat caaaggcggc actgtaaaag tgaccggtat
     4801 tggacgtaac agtatgcagg gtgatattcg ctttgctgat gtgctggaaa aaatgggcgc
     4861 gaccatttgc tggggcgatg attatatttc ctgcacgcgt ggtgaactga acgctattga
     4921 tatggatatg aaccatattc ccgatgcggc gatgaccatt gccacggcgg cgttatttgc
     4981 aaaaggcacc accacgctgc gcaatatcta taactggcgt gttaaagaaa ccgatcgcct
     5041 gtttgcgatg gcaacagaac tgcgtaaagt cggtgcggaa gtagaagagg ggcacgatta
     5101 cattcgtatc actccaccgg aaaaactgaa ctttgccgag atcgcgacat acaatgatca
     5161 ccggatggcg atgtgtttct cgctggtggc gttgtcagat acaccagtga cgattcttga
     5221 tcccaaatgt acggccaaaa catttccgga ttatttcgag cagttggcgc ggattagcca
     5281 ggcagcctga atgaacaacg ggcaataaat agccaaatct ttctttatca aaacgtcggc
     5341 acattgtcgg cgtttttttt cggaccttgt gagtcatttt gattaatggt agcgtcgctt
     5401 gtcaatgtaa gttgttgata cataatattt atatatgatt aatcaacgga tgattcacat
     5461 gaagaatact aaattactgc tggcgattgc gacctctgca gcattactga cagggtgtca
     5521 aaatacccac ggtattgata ccaatatggc tatcagctcc ggtttaaatg cctataaagc
     5581 agcaacatta agcgatgccg atgcaaaagc gattgccaat cagggctgtg ccgaaatgga
     5641 cagcggcaat caagtcgcaa gtaaatccag caagtacggt aaacgtctgg caaaaatcgc
     5701 caaagcattg ggtaacaata ttaatggcac gccggtcaac tataaggttt atatgaccag
     5761 cgacgtcaac gcatgggcga tggcgaacgg ctgtgtccgt gtctacagtg gcctgatgga
     5821 catgatgaat gacaacgaaa ttgaaggtgt tctgggccat gaactgggcc acgtcgcgtt
     5881 gggtcactcg ctggctgaaa tgaaagcttc ttatgcgatc gttgccgcac gcgatgccat
     5941 ttcagctacc agcggagtgg cttcccagct ttcccgctca caattgggtg atatcgcaga
     6001 aggcgctatt aatgctaaat actcccgtga taaagagtcc gaagcagatg atttctcctt
     6061 tgatctgttg aagaaacgtg gcatcagcac ccaggggctg gttggcagct ttgaaaaact
     6121 ggctagtctg gatggcggtc gcacccagtc catgtttgac tctcacccac catcaacaga
     6181 gcgtgcgcaa cacatccgtg atcgtatcgc ctctggtaag taaatcattg tcatctttcg
     6241 ggctggtctt ctgccagccc gctataattg cgcaataaat ccccatctga atacagacaa
     6301 aactggtttt tgcacacaac gttaacgatt tgtggcgtcg gcgcgtataa tgcgcgcggt
     6361 tatgttaacg gtacgcctgt tttaaggaga taaagatgac ggcaattgcc ccggttatta
     6421 ccattgatgg cccaagcggt gcagggaaag gcaccttgtg taaggctatg gcggaagcat
     6481 tgcaatggca tctgctggat tcgggtgcaa tttatcgcgt actggcattg gcggcattac
     6541 atcaccatgt tgatgttgcg tcagaagatg cgctggtacc gctggcatcc catctggatg
     6601 tacgttttgt gtcaaccaat ggcaatctgg aagtgatcct cgaaggggaa gatgtcagcg
     6661 gcgaaattcg tactcaggaa gtggcgaatg cagcttcaca agtcgcggca ttcccacgcg
     6721 ttcgtgaagc attattgcgt cgccaacgcg cgtttcgcga attaccaggc ctgattgccg
     6781 atggccgcga catgggaacg gtggtattcc ctgatgcgcc agtgaaaatt ttccttgacg
     6841 cctcctcgga agaacgtgcg catcgccgca tgctacagtt gcaggagaag ggctttagtg
     6901 ttaactttga gcgccttttg gccgagatca aagaacgcga cgaccgcgat cgtaaccgag
     6961 cggtagcgcc actggttccg gcagccgatg ctttagtgtt ggattccacc accttaagca
     7021 ttgagcaagt gattgaaaaa gcgctacaat acgcgcgcca gaaattggct ctcgcataag
     7081 cgaccgaatt tgcagtaccc ccgttgcaat ggaatgacag cgggtatgtt aaacaacccc
     7141 atccggcatg gagccaggtg gacgttaaat ataaacctga agattaaaca tgactgaatc
     7201 ttttgctcaa ctctttgaag agtccttaaa agaaatcgaa acccgcccgg gttctatcgt
     7261 tcgtggcgtt gttgttgcta tcgacaaaga cgtagtactg gttgacgctg gtctgaaatc
     7321 tgagtccgcc atcccggctg agcagttcaa aaacgcccag ggcgagctgg aaatccaggt
     7381 aggtgacgaa gttgacgttg ctctggacgc agtagaagac ggcttcggtg aaactctgct
     7441 gtcccgtgag aaagctaaac gtcacgaagc ctggatcacg ctggaaaaag cttacgaaga
     7501 tgctgaaact gttaccggtg ttatcaacgg caaagttaag ggcggcttca ctgttgagct
     7561 gaacggtatt cgtgcgttcc tgccaggttc tctggtagac gttcgtccgg tgcgtgacac
     7621 tctgcacttg gaaggcaaag agcttgaatt taaagtaatc aagctggatc agaagcgcaa
     7681 caacgttgtt gtttctcgtc gtgccgttat cgaatccgaa aacagcgcag agcgcgatca
     7741 actgctggaa aacctgcagg aaggcatgga agttaaaggt atcgttaaga acctcactga
     7801 ctacggtgca ttcgttgatc tgggcggcgt tgacggcctg ctgcacatca ctgacatggc
     7861 ctggaaacgc gttaagcatc cgagcgaaat cgtcaacgtg ggcgacgaaa tcactgttaa
     7921 agtgctgaag ttcgaccgcg aacgtacccg tgtatccctg ggcctgaaac agctgggcga
     7981 agatccgtgg gtagctatcg ctaaacgtta tccggaaggt accaaactga ctggtcgcgt
     8041 gaccaacctg accgactacg gctgcttcgt tgaaatcgaa gaaggcgttg aaggcctggt
     8101 acacgtttcc gaaatggatt ggaccaacaa aaacatccac ccgtccaaag ttgttaacgt
     8161 tggcgatgta gtggaagtta tggttctgga tatcgacgaa gaacgtcgtc gtatctccct
     8221 gggtctgaaa cagtgcaaag ctaacccgtg gcagcagttc gcggaaaccc acaacaaggg
     8281 cgaccgtgtt gaaggtaaaa tcaagtctat cactgacttc ggtatcttca tcggcctgga
     8341 cggcggcatc gacggcctgg ttcacctgtc tgacatctcc tggaacgttg caggcgaaga
     8401 agcagttcgt gaatacaaaa aaggcgacga aatcgctgca gttgttctgc aggttgacgc
     8461 agaacgtgaa cgtatctccc tgggcgttaa acagctcgca gaagatccgt tcaacaactg
     8521 ggttgctctg aacaagaaag gcgctatcgt aaccggtaaa gtaactgcag ttgacgctaa
     8581 aggcgcaacc gtagaactgg ctgacggcgt tgaaggttac ctgcgtgctt ctgaagcatc
     8641 ccgtgaccgc gttgaagacg ctaccctggt tctgagcgtt ggcgacgaag ttgaagctaa
     8701 attcaccggc gttgatcgta aaaaccgcgc aatcagcctg tctgttcgtg cgaaagacga
     8761 agctgacgag aaagatgcaa tcgcaactgt taacaaacag gaagatgcaa acttctccaa
     8821 caacgcaatg gctgaagctt tcaaagcagc taaaggcgag taattctctg actcttcggg
     8881 atttttattc cgaagtttgt tgagtttact tgacagattg caggtttcgt cctgtaatca
     8941 agcactaagg gcggctacgg ccgcccttaa tcaatgcagc aacagcagcc gcttaatttg
     9001 cctttaagga accggaggaa tcatgaccaa gtcagaattg atagaaagac ttgccaccca
     9061 gcaatcgcac attcccgcca agacggttga agatgcagta aaagagatgc tggagcatat
     9121 ggcctcgact cttgcgcagg gcgagcgtat tgaaatccgc ggtttcggca gtttctcttt
     9181 gcactaccgc gcaccacgta ccggacgtaa tccgaagact ggcgataaag tagaactgga
     9241 aggaaaatac gttcctcact ttaaaccagg taaagaactg cgcgatcgcg ccaatattta
     9301 cggttaagtt tttactcaaa cttgaacgcg agaaaagcac ctgtcgggtg cttttttcat
     9361 ttctctaatc aggaactgga agctgcctcg cagagttttg agcagttttc accctctcgt
     9421 taaattcttc tgaacgtgcc tcggggaacg caaaattccc accagacaac cgctcaacaa
     9481 agttgcacac tttccataaa cagggagggg tgcatgaaaa taacgacagt cggcgtatgc
     9541 ataatttgcg gaatttttcc gttgctgatt ttgccccaat tgcctgggac attaaccctt
     9601 gcgtttctga ctctcttcgc ctgcgtactg gcatttatcc ctgttaaaac cgtccgttat
     9661 atcgcgctga cgttgttgtt tttcgtttgg ggcatattag cagcaaagca aattttgtgg
     9721 gcaggagaaa ccttaactgg cgcgacgcag gatgcaattg ttgagatcac tgctactgac
     9781 ggtatgacca ctcattacgg tcaaattact catctacaag gtcgacgtat attccctgcg
     9841 ccaagccttg tgctgtatgg cgaatatctt ccgcaagcgg tttgtgccgg acaactgtgg
     9901 tcaatgaaac tcaaagttcg tgccgttcat ggtcaactta atgatggcgg ctttgatagc
     9961 cagcgttatg ccattgccca gcatcagccg ctcaccggcc gctttctgca ggcaagtgtt
    10021 attgaaccga attgtagcct gcgtgcacag tatctggcgt cactacaaac aacgctgcaa
    10081 ccctatccgt ggaatgcggt tattcttggt ttaggtatgg gggaacggtt atccgtccct
    10141 aaagaaatca aaaatatcat gcgtgatact ggaacggcgc atttaatggc gatatcggga
    10201 ttgcacatcg cttttgcggc gttgctggct gccggactca ttcgcagtgg acaaattttt
    10261 ctgcctgggc gctggatcca ctggcaaatg ccattaattg gcggaatctg ctgtgctgct
    10321 ttttatgcct ggctgacggg aatgcaacct cctgcattgc gtaccgttgt ggcgcttgct
    10381 acgtggggaa tgcttaagtt aagtgggcga cagtggagtg gctgggatgt atggatatgt
    10441 tgtctggcgg caattttgct gatggatcct gttgccattc tctcgcaaag tttatggctc
    10501 tctgccgctg cggtcgcggc attgatattt tggtatcagt ggtttccctg tcctgagtgg
    10561 caactgccgc cggtattgcg tgcagttgtt tccctcatcc atctgcaact gggaatcaca
    10621 cttctgctta tgcccgtgca aatcgtcata tttcatggca ttagtctgac ctcgtttatt
    10681 gcaaatctat tagcaattcc cctggtgaca tttatcacgg ttccgttgat cctcgccgcg
    10741 atggttgtgc atttaagcgg gccgttaatc ctggagcaag ggttatggtt tcttgccgac
    10801 cggtctttgg ctttactttt ctgggggtta aagagtttgc cggaagggtg gatcaacatt
    10861 gctgaatgtt ggcaatggct atcattttcc ccatggttct tactggtggt atggcgatta
    10921 aacgcctggc gaacgttgcc agcaatgtgt gtggctggag gcttgctgat gtgctggccg
    10981 ctgtggcaaa aacctcgacc tgacgagtgg cagctgtaca tgcttgatgt cgggcaaggg
    11041 ctggcaatgg tgatagccag aaacggcaaa gcgattctct atgacacagg actggcctgg
    11101 cccgaagggg atagtgggca acaactgatt atcccctggc tccactggca taatcttgaa
    11161 ccggaaggcg ttattctgag tcatgaacat ctggatcacc ggggagggct ggactcaata
    11221 ttgcacacat ggccgatgtt atggatcaga agtccgttaa actgggaaca tcatcagccc
    11281 tgtgtgcgtg gcgaagcgtg gcaatggcaa ggattgcgtt tcagcgtgca ctggccttta
    11341 caagctagca acgataaagg aaataaccat tcctgtgtgg ttaaggttga tgacgggacg
    11401 aatagcattc ttctaaccgg tgatattgaa gtccccgctg aacaaaagat gctaagccgt
    11461 tactggcagc aagtgcagac aacattgctt caggtacctc accatggcag taatacctca
    11521 tcatcgttgc cattaattca gcgagtgaat ggaaaagtgg cactcgcatc ggcatcgcgc
    11581 tataacgcat ggcgattgcc ctctaataaa gttaagcatc gctatcaaca gcaaggatat
    11641 taatggcttg atactccaca tcagggtcaa gtgacggtca atttttcagc gcaaggctgg
    11701 cggattagca gcctcagaga gcaaatttta cctcgttggt atcatcagtg gtttggcgtg
    11761 ccagtggata acgggtagaa tatgcggcta tttcaacaaa tgctggtttt ttgaatgcat
    11821 aacgacaaag atctctctac gtggcagacg ttccgccgac tgtggccaac cattgcgcct
    11881 tttaaagcgg gtctgatcgt ggcgggcgta gcgttaatcc tcaacgcagc cagcgatacc
    11941 ttcatgttat cgctccttaa gccacttctt gatgatggct ttggtaaaac agatcgctcc
    12001 gtgctggtgt ggatgccgct ggtggtgatc gggctgatga ttttacgtgg tatcaccagc
    12061 tatgtctcca gctactgtat ctcctgggta tcaggaaagg tggtaatgac catgcgtcgc
    12121 cgcctgtttg gtcacatgat gggaatgccg gtttcatttt ttgacaaaca gtcaacgggg
    12181 acgctgttgt cacgtatcac ctacgattcc gaacaggttg cttcttcttc ttccggcgca
    12241 ctgataacgg ttgtgcgtga aggtgcgtca atcatcggcc tgttcatcat gatgttctat
    12301 tacagttggc aactgtcgat cattttgatt gtgctggcac cgattgtttc gattgcgatt
    12361 cgcgtagtat cgaagcgttt tcgcaacatc agtaaaaaca tgcagaacac catggggcag
    12421 gtgaccacca gcgccgaaca aatgctgaag ggccacaaag aagtattgat tttcggtggt
    12481 caggaagtgg aaacgaaacg ctttgataaa gtcagcaacc gaatgcgtct tcaggggatg
    12541 aaaatggttt cagcctcttc catctctgat ccgatcattc agctgatcgc ctctttggcg
    12601 ctggcgtttg ttctgtatgc ggcgagcttc ccaagtgtca tggatagcct gactgccggt
    12661 acgattaccg ttgttttctc ttcaatgatt gcactgatgc gtccgctgaa atcgctgacc
    12721 aacgttaacg cccagttcca gcgcggtatg gcggcttgtc agacgctgtt taccattctg
    12781 gacagtgagc aggagaaaga tgaaggtaag cgcgtgatcg agcgtgcgac tggcgacgtg
    12841 gaattccgca atgtcacctt tacttatccg ggacgtgacg tacctgcatt gcgtaacatc
    12901 aacctgaaaa ttccggcagg gaagacggtt gctctggttg gacgctctgg ttcgggtaaa
    12961 tcaaccatcg ccagcctgat cacgcgtttt tacgatattg atgaaggcga aatcctgatg
    13021 gatggtcacg atctgcgcga gtataccctg gcgtcgttac gtaaccaggt tgctctggtg
    13081 tcgcagaatg tccatctgtt taacgatacg gttgctaaca acattgctta cgcacggact
    13141 gaacagtaca gccgtgagca aattgaagaa gcggcgcgta tggcctacgc catggacttc
    13201 atcaataaga tggataacgg tctcgataca gtgattggtg aaaacggcgt gctgctctct
    13261 ggcggtcagc gtcagcgtat tgctatcgct cgagccttgt tgcgtgatag cccgattctg
    13321 attctggacg aagctacctc ggctctggat accgaatccg aacgtgcgat tcaggcggca
    13381 ctggatgagt tgcagaaaaa ccgtacctct ctggtgattg cccaccgctt gtctaccatt
    13441 gaaaaggcag acgaaatcgt ggtcgtcgag gatggtgtca ttgtggaacg cggtacgcat
    13501 aacgatttgc ttgagcaccg cggcgtttac gcgcaacttc acaaaatgca gtttggccaa
    13561 tgatcgaaaa aatctggtct ggtgaatccc ctttgtggcg gctattgctg ccactctcct
    13621 ggttgtatgg cctggtgagt ggcgcgatcc gtctttgcta taaactaaaa ctgaagcgcg
    13681 cctggcgtgc ccccgtaccg gttgtcgtgg ttggtaatct caccgcaggc ggcaacggaa
    13741 aaaccccggt cgttgtctgg ctggtggaac agttgcaaca gcgcggtatt cgcgtggggg
    13801 tcgtatcgcg gggatatggt ggtaaggctg aatcttatcc gctgttattg tcggcagata
    13861 ccacaacagc acaggcgggt gatgaacctg tgttgattta tcaacgcact gatgcgcctg
    13921 ttgcggtttc tcccgttcgt tctgatgcgg taaaagccat tctggcgcaa caccctgatg
    13981 tgcagatcat cgtaaccgac gacggtttac agcattaccg tctggcgcgt gatgtggaaa
    14041 ttgtcgttat tgatggtgtg cgtcgctttg gcaatggctg gtggttgccg gcggggccaa
    14101 tgcgtgagcg agcggggcgc ttaaagtcgg ttgatgcggt aatcgtcaac ggcggtgtcc
    14161 ctcgcagcgg tgaaatcccc atgcatctgc tgccgggtca ggcggtgaat ttacgtaccg
    14221 gtacgcgttg tgacgttgct cagcttgaac atgtagtggc gatggcgggg attgggcatc
    14281 cgccgcgctt ttttgccacg ctgaagatgt gtggcgtaca accggaaaaa tgtgtaccgc
    14341 tggccgatca tcagtctttg aaccatgcgg atgtcagtgc gttggtaagc gccgggcaaa
    14401 cgctggtaat gactgaaaaa gatgcggtga aatgccgggc ctttgcagaa gaaaattggt
    14461 ggtatttgcc tgtagacgca cagctttcag gtgatgaacc agcgaaactg cttacgcaac
    14521 taacctcgct ggcttctggc aactagttac gccgcggcag cgttcgattg atggagtcat
    14581 gaatgtcgct gccgcacctc tcccttgctg atgcgcgtaa tcttcacctt gccgcacaag
    14641 gcctgttaaa caaaccccgc cgtcgagcgt cgttggagga tattccggca acgatctccc
    14701 gcatgtcctt gctgcaaatc gataccatca atattgttgc ccgtagtcca tatctggtgc
    14761 ttttcagtcg tctgggaaat tatcctgccc agtggctgga tgagtctctg gcgcgtggcg
    14821 aattaatgga atactgggcg catgaagcct gctttatgcc gcgtagcgac tttcgtctta
    14881 ttcgccaccg catgctggca cctgaaaaaa tgggctggaa atacaaagac gcctggatgc
    14941 aggaacatga ggcggaaatt gcacagttaa ttcagcatat tcatgataag gggccggtac
    15001 gttcagccga ttttgagcat cctcgtaaag gtgcaagcgg ctggtgggaa tggaagccgc
    15061 ataaacggca tctggaaggt ttatttactg ccggaaaggt gatggtgatt gaacggcgca
    15121 acttccagcg cgtttatgat ttaacccacc gtgtcatgcc tgactgggat gatgagcgcg
    15181 atctcgtttc gcaaacagaa gcagaaatca tcatgctgga taacagtgcg cgtagcctgg
    15241 gaatattccg cgaacagtgg ctggcagatt actatcggct gaaacgtccg gcactggcgg
    15301 cgtggcgcga agcgagggct gaacagcagc aaatcattgc tgtgcatgtt gaaaaattgg
    15361 gcaatctttg gctgcatgct gatttgctgc cgctactcga gcgtgcgctg gccggaaagc
    15421 tcactgcaac gcacagcgcg gtactttcgc cttttgatcc tgttgtctgg gatcgcaaac
    15481 gcgcagagca gctttttgat tttagctacc ggctggagtg ctatacccca gcgccgaaac
    15541 gccagtatgg ctattttgtt ctgccattat tacatcgtgg gcaattagtt gggcgaatgg
    15601 atgccaaaat gcatcgccag acaggcatcc ttgaagttat ctctctgtgg ttacaggaag
    15661 gcattaaacc aacgacaatg ctgcaaaaag ggttacgtca ggcgattact gatttcgcga
    15721 gctggcagca ggcaacgcgg gtgacattag gacgctgccc gcaaggtctc tttactgatt
    15781 gccgcactgg ctgggaaata gaccccgtcg cataaatgat tatgataaga tttatgcatt
    15841 cataacccga tccatctgga ggaactatgg atcatcgttt gcttgaaatc attgcctgcc
    15901 cggtttgcaa cggaaaactt tggtataacc aggaaaaaca agagcttatt tgcaaactgg
    15961 ataacctggc tttcccgctc cgcgatggca ttccagtgtt gctggaaacg gaagcccgcg
    16021 tgctgactgc tgatgagagt aaatcatgag ttttgtggtc attattcccg cgcgctacgc
    16081 gtccacgcgt ctgcccggta aaccattggt tgatattaac ggcaaaccca tgattgttca
    16141 tgttcttgaa cgcgcgcgtg aatcaggtgc cgagcgcatc atcgtggcaa ccgatcatga
    16201 ggatgttgcc cgcgccgttg aagccgctgg cggtgaagta tgtatgacgc gcgccgatca
    16261 tcagtcagga acagaacgtc tggcggaagt tgtcgaaaaa tgcgcattca gcgacgacac
    16321 ggtgatcgtt aatgtgcagg gtgatgaacc gatgatccct gcgacaatca ttcgtcaggt
    16381 tgctgataac ctcgctcagc gtcaggtggg tatggcgact ctggcggtgc caatccgcaa
    16441 tgcggaagaa gcgtttaacc cgaatgcggt gaaagtggtt ctcgacgctg aagggtatgc
    16501 actgtacttc tcacgcgcca ccattccttg ggatcgtgat cgttttgcag aaggccttga
    16561 aaccgttggc gataacttcc tgcgtcatct tggtatttat ggctaccgtg caggctttat
    16621 ccgtcgttac gtcaactggc aggcaagtcc gttagaacac atcgaaatgt tagagcagct
    16681 tcgtgttctg tggtacggcg aaaaaatcca tgttgctgtt gcgcatgaag ttcctggcac
    16741 aggtgtggat acccctgaag atctcgagcg cgttcgcgct gaaatgcgct aatttcactt
    16801 cacgacactt cagccaattt tgggaggagt gtcgtaccgt tacgattttc ctcaattttt
    16861 cttttcaaca attgatctca ttcaggtgac atcttttata ttggcgctca ttatgaaagc
    16921 agtagctttt atgagggtaa tctgaatgga acagctgcgt gccgaattaa gccatttact
    16981 gggcgaaaaa ctcagtcgta ttgagtgcgt caatgaaaaa gcggatacgg cgttgtgggc
    17041 tttgtatgac agccagggaa acccaatgcc gttaatggca agaagcttta gtacgcccgg
    17101 aaaagcccga caactggcat ggaaaaccac catgctggca agaagtggga ctgtccgtat
    17161 gccgactatt tatggtgtga tgacgcatga agaacacccc ggccccgatg tcctgctact
    17221 ggagcggatg cgtggtgttt cggtggaggc accagcccga acaccagaac gctgggaaca
    17281 actcaaagac caaatcgttg aagccttact ggcctggcac cgtcaggaca gtcgcggttg
    17341 cgtcggcgcg gtcgacaata ctcaggaaaa tttctggccc tcatggtacc ggcaacatgt
    17401 tgaagtgcta tggaccacgc tcaatcagtt caataacacc ggtctgacga tgcaggataa
    17461 gcggatcctg tttcgcactc gcgaatgtct cccggcatta tttgaaggct ttaacgacaa
    17521 ttgtgtgttg attcacggta acttctgttt acgcagcatg ctgaaagatt cgcgcagcga
    17581 tcagttactg gcgatggtcg ggccgggact aatgctttgg gcaccgcgag aatacgaact
    17641 gttccgacta atggataatt ctctggcgga agatttgctc tggagttacc tgcaacgcgc
    17701 gccagtggcg gagtcgttca tctggcggcg ttggttgtat gtgttatggg atgaagttgc
    17761 gcaactggtt aataccggac gatttagtcg gcgcaacttc gatctggcat caaaatcact
    17821 cttgccgtgg ctcgcctgac ggacctttca gccactgcca gatgcggcca agcgtttcat
    17881 agccaacacg atcgctatgc atcagccaga ccggagaagg gattgcccgt tcccacggat
    17941 taagcggcga gtcgatggcc aactgattcg ccggggctgg tagcggattt aacccttcct
    18001 gctgaaaaaa gatcatcgcc cgcggcagat gtgatgcgga ggtcaccagc aggaaagggg
    18061 cgtcaccaat cgcctgtttc actgccgcag cttcttcttc ggtatctttt ggcaaatcca
    18121 gggtgataat ttgctcgcgc ggcacgccca gcgattgcgc aactctggca cctacttccg
    18181 ctgtactcac cgtattggtt tttgctacgc ctcccgtgaa gatcagtttt gatcccggat
    18241 tttcgcgcca taagcgaata ccttcattca ggcgtggcag actgttattg attaaattag
    18301 agctcggtgc ccactgtggg ttccaggtat aaccacctcc gagcaccacg atatagtcca
    18361 ctttttgcga attattccat gtcggatagg tgctttcgat gggacgcagc aggcggtcgg
    18421 ctaccggttg caaactcaat aacaagagtg ccagccaccc aatgctaata aaaatcttcc
    18481 cggttttttg aaaacggcta aaccacagca gcgccaggcc agcgccaata attaacaaca
    18541 tcagcggaag tggcagcaac atattgccaa tcactttttt cagtgtaaaa agcatccttt
    18601 ctggttcctt ttttaaccat atagcaaggg atcgccagcg atattacacc agacgggttc
    18661 attctcgccg tggctgtgac aaaatagcgt ctttatcaga aaacgtctta gccaaccggt
    18721 ggagaaagca atgcaggatc gcaatttcga tgatattgcg gaaaagtttt cccgtaacat
    18781 ttacggcacc accaaagggc agcttcgaca ggctattctg tggcaggatc tcgatcacgt
    18841 gctggcggaa atgggcccgc aaaaactgcg tgtgctggat gctggcggtg gagaagggca
    18901 gaccgcaatc aaaatggccg agcgtgggca tcaggtcatt ttatgcgatc tttctgcgca
    18961 gatgatcgac cgcgcaaaac aggcggcaga agcaaaaggt gtgagcgaca acatgcaatt
    19021 tatacattgc gccgctcagg atgttgcttc gcatttggaa acgcccgttg atctgatatt
    19081 gttccatgcg gtgctcgagt gggtggccga tccccgcagt gtattgcaga ccctgtggtc
    19141 agtgttgcgt ccaggcggag tgttgtcgtt aatgttctac aatgcgcatg gtttgttgat
    19201 gcataacatg gtcgccggga attttgatta cgtgcaggcg ggaatgccga aaaagaaaaa
    19261 acggacgctt tcgccagatt atccacgcga cccggcgcag gtttatctgt ggctggaaga
    19321 agctggttgg caaattatgg gtaagacagg cgttcgcgtg tttcatgatt atctgcgcga
    19381 gaagcaccag cagcgcgact gctatgaagc attacttgaa ttagaaacgc gttattgccg
    19441 tcaggaaccg tatattaccc tggggcgtta tattcatgtc accgcgcgca aaccgcagag
    19501 caaggataaa gtatgagtga attttcccag acagtccccg aactggttgc ctgggccaga
    19561 aaaaatgact tctccatctc gctgccggta gaccgactct cttttctgct ggcggttgcc
    19621 acgctgaacg gcgagcgtct ggatggtgag atgagtgaag gcgagctggt ggatgcattc
    19681 cgccatgtga gtgatgcgtt tgagcaaacc agcgaaacca tcggcgtgcg cgccaacaac
    19741 gcgatcaacg acatggtgcg tcaacgtctg ctgaaccgct ttaccagcga gcaggcggaa
    19801 gggaacgcaa tttaccgtct gacgccgctc ggcatcggca tcactgacta ttacattcgt
    19861 cagcgcgagt tttctacgct gcgcctttct atgcagttgt cgattgtggc gggtgagctc
    19921 aaacgcgcag cagatgccgc cgaagagggc ggtgatgaat ttcactggca ccgtaatgtt
    19981 tatgctccac tgaaatattc ggtagcagaa attttcgaca gtatcgacct gacgcaacgt
    20041 ctgatggatg aacagcagca gcaggtgaag gatgatatcg cccagttgct gaacaaagac
    20101 tggcgggcgg cgatttccag ctgtgaattg ttgctttcgg aaacttccgg aacgctgcgt
    20161 gaattgcagg atacgctgga agcggcaggc gacaaattgc aggctaatct gttgcgcatt
    20221 caggatgcga cgatgaccca tgacgatctg catttcgtcg atcgtctggt gttcgatctg
    20281 cagagcaaac tcgatcgtat tatcagttgg ggccagcaat ccatcgactt gtggattggc
    20341 tacgaccgcc acgtacacaa atttattcgt accgcgatcg atatggataa aaaccgcgtc
    20401 tttgctcagc ggttacgtca gtcggtacaa acctattttg atgagccgtg ggcgctaact
    20461 tatgccaatg ccgatcgtct gctggatatg cgtgacgaag agatggcact gcgcgatgaa
    20521 gaagtgactg gggaacttcc tgaggatctg gaatacgaag agtttaacga gatccgcgaa
    20581 cagctggcgg cgatcatcga agaacaactt gccgtgtaca aaaccagaca agtgccgctg
    20641 gatcttggtc tggtggtacg cgaatatctg tcacagtatc cgcgtgcacg tcactttgac
    20701 gttgcgcgta ttgttattga tcaggcggta cgtcttggcg tagcgcaagc agatttcacc
    20761 ggactgccag cgaaatggca gccgattaat gattacggag ccaaggtaca ggcgcatgtc
    20821 atcgacaaat attgaacaag tgatgccggt taagctggcg caggcgctgg cgaatccgtt
    20881 atttccggcg ctggacagcg ccttacgttc aggccgccat attggcctcg acgaactgga
    20941 taatcatgca ttcctgatgg attttcagga atatctggaa gagttttacg cgcgttataa
    21001 cgttgagctt attcgcgcac cagaagggtt cttctattta cgcccacgtt ccaccacgct
    21061 gatccctcgt tccgtcttgt cggaactgga tatgatggtc gggaaaatcc tctgttatct
    21121 ctatctcagc ccggaacggc tggcgaatga ggggattttc acccagcagg aactgtacga
    21181 cgaactgctc accctggccg atgaagcaaa actgctgaaa ctggtgaaca accgttcaac
    21241 cggttcagac gttgaccgtc agaagttgca ggagaaagta cgttcttcgc tcaaccgtct
    21301 gcgtcgttta ggcatggtgt ggtttatggg ccacgacagc agcaagtttc gcattaccga
    21361 atcggtgttc cgcttcggag ccgatgtgcg tgctggcgac gatccccgtg aagcacagcg
    21421 tcgcctgatt cgtgatggcg aagcaatgcc gattgaaaat catctgcaac tcaacgatga
    21481 aaccgaagag aatcagccag atagcggaga ggaagaataa tgattgaacg cggtaaattt
    21541 cgctcactga cgctgattaa ctggaacggc ttttttgccc gaacttttga ccttgacgag
    21601 ctggtcacga cgctttctgg cggtaacggg gcgggtaaat ccaccaccat ggcggcgttc
    21661 gttacggcgc tgatccccga cctgaccctg ctgcatttcc gtaacactac ggaagccggg
    21721 gccaccagcg gttcgcgcga taaaggtctg cacggtaagc tgaaagcggg tgtctgttat
    21781 tcgatgctcg acaccattaa ctcgcgccac cagcgcgtgg tggtcggtgt gcgtctgcaa
    21841 caggttgccg gacgcgatcg taaagtggat atcaaaccat ttgccattca gggactgccg
    21901 atgtcggtgc agccgacaca gctggtgacc gaaaccctga acgaacgcca ggcgcgcgtg
    21961 ctgccgctta acgagctgaa agacaagctc gaggcgatgg aaggcgtgca gtttaaacag
    22021 ttcaactcca ttaccgatta ccactcgctg atgttcgatc ttggcatcat cgcgcgtcgt
    22081 ctgcgctccg catctgaccg tagcaaattc tatcgtctga tcgaagcttc gctgtatggc
    22141 gggatctcca gtgccattac ccgttctctg cgcgactacc tgttgccgga aaacagcggc
    22201 gtgcgtaaag cgttccagga catggaagcg gcgctgcgtg aaaaccgcat gacgctggaa
    22261 gcgattcgtg tcacccagtc ggaccgtgac ctgtttaagc atctgatcag cgaagctacc
    22321 aactacgtgg cggcggacta catgcgtcac gccaacgagc gccgtgtcca tctcgacaaa
    22381 gccctggagt ttcgtcgcga gttacatact tcgcgtcagc aactggcggc tgagcagtac
    22441 aaacacgtcg atatggcgcg tgagctggca gagcacaacg gtgccgaagg cgatctggaa
    22501 gcggattatc aggcggccag cgatcacctg aacctggtgc aaaccgcact gcgtcagcag
    22561 gagaagatcg aacgctacga agcggatctc gatgagctgc agatccgtct ggaagagcaa
    22621 aacgaagtgg tggcagaagc catcgaacgc caggaagaga acgaggctcg cgcggaagct
    22681 gccgaactgg aagtggacga gctgaaaagc cagcttgctg actaccagca ggcgctggac
    22741 gtccagcaaa cgcgcgcgat ccagtataac caggcgattg ctgcgcttaa tcgcgccaaa
    22801 gaactgtgcc atctgccgga cttaacctcc gacagcgccg ccgaatggct ggaaaccttc
    22861 caggcgaaag agctggaagc gactgaaaaa atgctctctc ttgagcagaa aatgagcatg
    22921 gcgcaaaccg cgcacagcca gtttgagcag gcttatcagc tggtggtggc aatcaacggc
    22981 ccgctggcgc gtaacgaggc gtgggatgtc gctcgcgaac tattgcgcga aggggtcgat
    23041 cagcgtcacc tggcagagca ggttcagccg ttgcggatgc gcttaagcga actggaacag
    23101 cgtctgcgcg aacagcaaga agctgagcgt ctgctggcag atttctgcaa acgtcagggc
    23161 aagaattttg acattgacga gctggaagct ctgcatcagg agctggaagc gcgcattgcc
    23221 tctctttccg atagcgtgtc taacgcccgt gaagagcgca tggcactgcg ccaggaacag
    23281 gaacagctgc agtctcgcat tcagagtttg atgcagcgtg cgccggtttg gctggcagcg
    23341 caaaacagtc tcaaccagtt gagcgaacag tgcggcgaag agtttacctc cagccaggac
    23401 gtcaccgaat atctgcaaca gttgctggag cgtgagcgag aggcgattgt tgaacgcgat
    23461 gaagtgggcg cgcgcaaaaa cgccgtcgat gaagagatcg aacgtttaag ccagcctggc
    23521 ggctctgaag atcagcgtct gaacgcgctg gcggagcgtt ttggtggtgt gctgctgtca
    23581 gaaatttatg acgacgttag cctggaagat gcgccgtact tctcagcgct gtatggcccg
    23641 tcacgccacg ccatcgtggt gccagatctg tcacaggtaa ctgaacacct ggaaggcttg
    23701 accgattgcc cggaagatct ctatctgatc gaaggagatc cgcagtcatt cgatgacagc
    23761 gtgttcagcg ttgatgagct ggaaaaagcg gtagtggtga aaatcgccga tcgtcagtgg
    23821 cgttattcac gtttcccgga agtgccgctg tttggtcgtg ctgcgcgtga aagccgtatt
    23881 gaaagcctcc atgccgagcg tgaagtgctt tccgaacgct tcgccacgct ctcctttgat
    23941 gtacagaaaa ctcagcgtct gcatcaggcg ttcagccgct ttatcggcag tcatctggcg
    24001 gttgcgtttg agtctgaccc ggaagcagaa atccgtcaac tgaacagccg tcgcgtcgaa
    24061 ctggagcggg cgttgagtaa tcatgaaaat gataaccagc agcagcgtat tcagtttgag
    24121 caggcgaaag aaggcgttac ggcgctgaac cgcattctgc cgcgtctcaa cctgttggct
    24181 gatgacagcc tggcggatcg cgtcgatgaa atccgcgaac gtctggatga agcccaggaa
    24241 gccgcgcgtt ttgttcagca gtttggcaat caactggcga aactggaacc gatcgtttcg
    24301 gtattgcaga gcgacccgga acagttcgaa cagctaaaag aagattacgc gtactctcag
    24361 cagatgcagc gcgatgcccg tcagcaggcg tttgccctga cggaagtggt gcagcgtcgt
    24421 gcgcacttta gctattctga ctcggcagaa atgctgagcg gtaatagcga tctcaacgaa
    24481 aagctgcgtg aacgtctgga acaggcggaa gcggagcgta ctcgcgctcg tgaagcgttg
    24541 cgtggtcacg cagcgcagtt gagtcagtac aaccaggtgc tggcttcgct gaaaagttct
    24601 tacgacacca aaaaagagct actcaacgat ctgcaacgtg aattgcagga tatcggcgtg
    24661 cgtgctgata gcggggcaga agagcgggcg cgtattcgcc gtgacgagct gcatgcgcaa
    24721 ctgagcaata accgttcacg ccgcaatcaa ctggaaaaag cgcttacctt ctgcgaagcg
    24781 gagatggaca acctgacccg caaactgcgc aagctggagc gggattactt tgagatgcgc
    24841 gagcaggtag tgaccgccaa agcgggctgg tgtgcggtga tgcgcatggt gaaagataac
    24901 ggcgttgagc gccgcttaca ccgtcgtgag ctggcttatc tctccgctga tgatttgcgc
    24961 tctatgtcgg ataaggcatt gggtgcgctg cgtctggcgg tggcggataa cgaacatctg
    25021 cgcgacgtgc tgcgcatgtc ggaagatccg aaacgtccgg agcgtaaaat tcagttcttc
    25081 gtggcggttt atcagcatct gcgtgaacgt attcgtcagg atattattcg taccgatgat
    25141 ccggtggaag ctatcgaaca gatggagatc gaacttagcc gtctgaccga agaattaacc
    25201 tcccgtgaac agaaactggc gatcagttcc cgcagcgtgg cgaacatcat tcgcaaaacc
    25261 attcagcgcg agcagaaccg tatccgtatg ctcaaccagg ggttgcagaa cgtatcgttt
    25321 ggtcaggtga acagcgtgcg cctcaacgtg aacgtgcgtg aaacgcacgc catgctgctg
    25381 gatgtgctct ctgaacagca cgagcagcat caggatctgt ttaacagcaa ccgtctgacc
    25441 ttctcggaag cgttggcgaa attgtatcag cgtcttaatc cgcagattga tatgggacag
    25501 cgcacgccgc agaccattgg cgaagaactg ctggattacc gcaactatct ggaaatggaa
    25561 gttgaggtta accgtggttc cgatggttgg ttgcgcgcag agtctggtgc attgtcgacc
    25621 ggtgaggcga ttggtaccgg tatgtcgatt ctggtgatgg tggtacaaag ctgggaagat
    25681 gaatctcgcc gcctgcgcgg taaagatatc tctccttgcc gcctgctgtt cctcgatgaa
    25741 gcagcgcgac tggatgctcg ttctatcgcc acgctgtttg aattgtgtga gcgtttgcaa
    25801 atgcagctca tcatcgcagc gccggaaaat atcagcccgg agaaaggcac cacctataaa
    25861 ctggtgcgta aagtcttcca gaataccgaa cacgttcacg tcgtcggcct gcgaggattt
    25921 gcgccgcaac tccctgaaac gcttccagga agtgacgaag cgccttctca ggcgagttaa
    25981 aattaaggcg gcagcaatgc cgccttttct ttttccgaaa actccgtttc cgcactaaaa
    26041 aagtggcaca ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg
    26101 tatcggcgtt tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt
    26161 cgtgagtatt ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg
    26221 ataaaaacag ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa
    26281 tcagtttgtg cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg
    26341 aagtaatccc tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg
    26401 cgcaagccac ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag
    26461 cccgcacgca aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc
    26521 ttcaactgtt gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta
    26581 aagccttcca gcaacagctg gcagaggtgg cgattgccgg tttccagccg cagtttaata
    26641 aatgggtaga gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct
    26701 ctgatgcgat gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct
    26761 ggctatatag cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat
    26821 ggcagctggc gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc
    26881 atccgcaata tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt
    26941 ggccccaact gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg
    27001 cgttgcgcga aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac
    27061 ctggcgatga cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag
    27121 cagaaactaa gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc
    27181 gtgccgccta cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat
    27241 tgggggcaga tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc
    27301 agcgtgctgg cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt
    27361 ctaccgggat catggttaac attccggcct attcgctggt ctactatcag aacggcaatc
    27421 aggtgctgga ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca
    27481 gtgcccttaa caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca
    27541 aagatattct gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg
    27601 tgatgcgcgg ctggaacagc agagaagcga ttgatccatg gcaggttgac tggtctacaa
    27661 tcacggcctc gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg
    27721 ggcgctataa attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc
    27781 acaatctgtt caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag
    27841 cttccgatct ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg
    27901 atgcgctgaa gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc
    27961 tctactacct gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt
    28021 acaattatga tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa
    28081 tcaggtaaat gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc
    28141 gattctctgc agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc
    28201 ggttaaggtg cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact
    28261 tgattatcat ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg
    28321 cactcggtgc cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc
    28381 gcattttgac actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg
    28441 gcagaggcta tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg
    28501 cgaacaaaat aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt
    28561 tgttaggcac gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca
    28621 atgaactacg cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg
    28681 cgatggattt ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta
    28741 tgcgcgcagg tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc
    28801 cagcacggca ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat
    28861 tccggtcacc gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc
    28921 cgcactggtc gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg
    28981 cctgacactg atgcagatcc tgctgacgca tggtcatctg gaccacgttg gtgcagcggc
    29041 ggaactggcg caacattacg gcgtgccggt ttttggtccg gaaaaagaag atgagttctg
    29101 gctgcaaggc ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac
    29161 gccagatcgt tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt
    29221 gttacattgc cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct
    29281 gctgatttct ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg
    29341 cgatcataat caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt
    29401 gacatttatt ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc
    29461 cttcctgcaa gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg
    29521 ggcctggatt tctgacaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc
    29581 cggcctacaa aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt
    29641 agcgcatcag gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat
    29701 tgtttttaat gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg
    29761 tgtcatcccg gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg
    29821 cagacgcagc acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat
    29881 gataaagctg aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg
    29941 catacgctga atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc
    30001 atcgttgctc aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt
    30061 agcgcgaatc gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc
    30121 aaccagagta caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact
    30181 ggcaacaatc agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc
    30241 cagaccacgg gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac
    30301 tgagagttgt gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg
    30361 gcagcagcca tggaacagca ctacgtcgcc agcctgagct tcgttcaggc tgttaatcag
    30421 tgcatcgaag tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag
    30481 acttgcagag ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg
    30541 cttaacgctg gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc
    30601 cggagtctgt gccgtgcgag cacgtttgtc attgatcagg gcgctacctt taccaaacag
    30661 cagttcctga gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt
    30721 ggtttcattt tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt
    30781 gcccgtctca tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc
    30841 acgaaacaga tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa
    30901 catgacgagg ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt
    30961 tacaaaattc caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc
    31021 tctggaaggt ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg
    31081 tgtgctatta gaactggaaa cgatacccac agcaacggtg tcgtctgaac ctacgcccag
    31141 tttgttgtca gaatcgatct ggttgatgat gtagtcaaca taggtggaca tgtttttgtt
    31201 gaagtagtag gttgcgccca cttcaaagta gttcaccaga tcaacatcac cgataccttc
    31261 tacgtctttc gctttagatt tggtgtaagc gatggacgga cgcagaccga aatcgaactg
    31321 gtattgcgca actaacagaa cgtcttgcgt tttgttggcg aagccgctgg tgtttgtaaa
    31381 tttattagtg atcggcgtag cgttacgggt ttcaccgtag ttcgctgcca agtagatgtt
    31441 gttcgcgtcg tacttcagac cagtagccca ctgttcagct tttttaccgt tgccaagagg
    31501 ttgagcttct tgcaggttgg tacggtcagc tgcaccataa gcaccaacga taccaaagcc
    31561 ttcgtattcg tagctgatag aaccgccaac accgtcgccg ttagaacggc gtgcagtgtc
    31621 acgctcgttt ttacccaggt actgaacagc gaagttcagg ccatcaacca gaccaaagaa
    31681 gttggagtta cgataggtag caacgccgcc aacacgacca acgaagaagt catcgctgta
    31741 tgcagtatca ccaccaaatt ctggcagcat atcggtgtaa cccagtgcat cataaaccac
    31801 accgtagtta cggccgtaat cgaaagaacc aacgtcagcg tatttaagac ccgcgaatgc
    31861 cagacgcgtt ttgttaccag tttgagcgtc agcgccttca gagttgttac cctggaagtt
    31921 atattcccac tgaccataac cggtcagatc ggaattgatt tgagtttccc ctttaaaacc
    31981 aagacgggca taggtcatgt cgccattgcc accgtaactg ttttcaccgt tacccttgga
    32041 aaaataatgc agaccaacag ctttaccgta cagatctact ttgttgccat ctttgttata
    32101 gatttctgca gcgtttgcag tacctgctac taacagagca gggacgatca ctgccagaat
    32161 attgcgcttc atcattattt attaccctca tggttttttt tatgacacct gccactgccg
    32221 tcaataagtt ctgtcaataa aaatttacgg aactattgat gagagtttgg tgtctttatg
    32281 tgtctgcagg catctttcca ttcaaactaa cgtttc
//
LOCUS       DBANUU010000045        31968 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000045 DBANUU010000000
VERSION     DBANUU010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.045"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            31..474
                     /gene="cmtB"
                     /locus_tag="ACIE68_002010"
     CDS             31..474
                     /gene="cmtB"
                     /locus_tag="ACIE68_002010"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFX5908327.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            502..1890
                     /gene="cmtA"
                     /locus_tag="ACIE68_002011"
     CDS             502..1890
                     /gene="cmtA"
                     /locus_tag="ACIE68_002011"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFX5908328.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1905..3182
                     /gene="yggP"
                     /locus_tag="ACIE68_002012"
                     /pseudo
     CDS             1905..3182
                     /gene="yggP"
                     /locus_tag="ACIE68_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            3179..4144
                     /gene="glpX"
                     /locus_tag="ACIE68_002013"
     CDS             3179..4144
                     /gene="glpX"
                     /locus_tag="ACIE68_002013"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFX5908329.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4166..4675
                     /gene="fumE"
                     /locus_tag="ACIE68_002014"
     CDS             4166..4675
                     /gene="fumE"
                     /locus_tag="ACIE68_002014"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFX5908330.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4672..5385
                     /locus_tag="ACIE68_002015"
     CDS             4672..5385
                     /locus_tag="ACIE68_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFX5908331.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5357..6034)
                     /locus_tag="ACIE68_002016"
     CDS             complement(5357..6034)
                     /locus_tag="ACIE68_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFX5908332.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(6028..6705)
                     /locus_tag="ACIE68_002017"
     CDS             complement(6028..6705)
                     /locus_tag="ACIE68_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFX5908333.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6693..7400)
                     /locus_tag="ACIE68_002018"
     CDS             complement(6693..7400)
                     /locus_tag="ACIE68_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFX5908334.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7401..7979)
                     /locus_tag="ACIE68_002019"
     CDS             complement(7401..7979)
                     /locus_tag="ACIE68_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFX5908335.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8002..8433)
                     /locus_tag="ACIE68_002020"
     CDS             complement(8002..8433)
                     /locus_tag="ACIE68_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFX5908336.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8805..9824
                     /gene="epd"
                     /locus_tag="ACIE68_002021"
     CDS             8805..9824
                     /gene="epd"
                     /locus_tag="ACIE68_002021"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFX5908337.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9874..11037
                     /gene="pgk"
                     /locus_tag="ACIE68_002022"
     CDS             9874..11037
                     /gene="pgk"
                     /locus_tag="ACIE68_002022"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFX5908338.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11252..12331
                     /gene="fbaA"
                     /locus_tag="ACIE68_002023"
     CDS             11252..12331
                     /gene="fbaA"
                     /locus_tag="ACIE68_002023"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFX5908339.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(12395..12493)
                     /locus_tag="ACIE68_002024"
                     /pseudo
     CDS             complement(12395..12493)
                     /locus_tag="ACIE68_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12689..13549
                     /gene="mscS"
                     /locus_tag="ACIE68_002025"
     CDS             12689..13549
                     /gene="mscS"
                     /locus_tag="ACIE68_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFX5908340.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13688..14323
                     /gene="argO"
                     /locus_tag="ACIE68_002026"
     CDS             13688..14323
                     /gene="argO"
                     /locus_tag="ACIE68_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFX5908341.1"
                     /translation="MFSYYFQGLALRAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14416..15156
                     /gene="yggE"
                     /locus_tag="ACIE68_002027"
                     /pseudo
     CDS             14416..15156
                     /gene="yggE"
                     /locus_tag="ACIE68_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
     gene            15323..16219
                     /gene="ygfI"
                     /locus_tag="ACIE68_002028"
     CDS             15323..16219
                     /gene="ygfI"
                     /locus_tag="ACIE68_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFX5908342.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEGRQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHKPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(16216..17694)
                     /gene="scpC"
                     /locus_tag="ACIE68_002029"
     CDS             complement(16216..17694)
                     /gene="scpC"
                     /locus_tag="ACIE68_002029"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFX5908343.1"
                     /translation="METQWTRMTANEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYQDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17718..18503)
                     /gene="scpB"
                     /locus_tag="ACIE68_002030"
     CDS             complement(17718..18503)
                     /gene="scpB"
                     /locus_tag="ACIE68_002030"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFX5908344.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18514..19509)
                     /gene="meaB"
                     /locus_tag="ACIE68_002031"
     CDS             complement(18514..19509)
                     /gene="meaB"
                     /locus_tag="ACIE68_002031"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFX5908345.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DTIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIINFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19502..21646)
                     /gene="scpA"
                     /locus_tag="ACIE68_002032"
     CDS             complement(19502..21646)
                     /gene="scpA"
                     /locus_tag="ACIE68_002032"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFX5908346.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEEVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALKAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(21850..22743)
                     /gene="argP"
                     /locus_tag="ACIE68_002033"
     CDS             complement(21850..22743)
                     /gene="argP"
                     /locus_tag="ACIE68_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFX5908347.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            22885..23115
                     /locus_tag="ACIE68_002034"
     CDS             22885..23115
                     /locus_tag="ACIE68_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908348.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RLLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            23171..23830
                     /gene="rpiA"
                     /locus_tag="ACIE68_002035"
     CDS             23171..23830
                     /gene="rpiA"
                     /locus_tag="ACIE68_002035"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFX5908349.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24086..25318
                     /gene="serA"
                     /locus_tag="ACIE68_002036"
     CDS             24086..25318
                     /gene="serA"
                     /locus_tag="ACIE68_002036"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFX5908350.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25547..25606
                     /gene="ibsC"
                     /locus_tag="ACIE68_002037"
     CDS             25547..25606
                     /gene="ibsC"
                     /locus_tag="ACIE68_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFX5908351.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            25939..25998
                     /locus_tag="ACIE68_002038"
     CDS             25939..25998
                     /locus_tag="ACIE68_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFX5908352.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26099..26701)
                     /gene="fau"
                     /locus_tag="ACIE68_002039"
     CDS             complement(26099..26701)
                     /gene="fau"
                     /locus_tag="ACIE68_002039"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFX5908353.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26722..26905)
                     /gene="ssrS"
                     /locus_tag="ACIE68_002040"
     ncRNA           complement(26722..26905)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACIE68_002040"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(26947..27276)
                     /gene="zapA"
                     /locus_tag="ACIE68_002041"
     CDS             complement(26947..27276)
                     /gene="zapA"
                     /locus_tag="ACIE68_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFX5908354.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27444..28022
                     /gene="ygfB"
                     /locus_tag="ACIE68_002042"
     CDS             27444..28022
                     /gene="ygfB"
                     /locus_tag="ACIE68_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFX5908355.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            28048..29373
                     /gene="pepP"
                     /locus_tag="ACIE68_002043"
     CDS             28048..29373
                     /gene="pepP"
                     /locus_tag="ACIE68_002043"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFX5908356.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29370..30548
                     /gene="ubiH"
                     /locus_tag="ACIE68_002044"
                     /gene_synonym="visB"
     CDS             29370..30548
                     /gene="ubiH"
                     /locus_tag="ACIE68_002044"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFX5908357.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAVL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30571..31773
                     /gene="ubiI"
                     /locus_tag="ACIE68_002045"
                     /gene_synonym="visC"
     CDS             30571..31773
                     /gene="ubiI"
                     /locus_tag="ACIE68_002045"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFX5908358.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDTTLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELVAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAAMMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31847..31930)
                     /locus_tag="ACIE68_002046"
     CDS             complement(31847..31930)
                     /locus_tag="ACIE68_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908359.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
BASE COUNT         7646 a         7912 c         8618 g         7792 t
ORIGIN      
        1 ggtaccctaa aaataaaata aggataattt atgcggctta gtgattattt tccagaatca
       61 tcaatctcag ttatacattc agcaaaagat tggcaggaag ctatcgattt ctcgatggta
      121 tcattgctgg ataaaaacta tatcagcgag aattacattc aggcaattaa agattccacc
      181 attaacaatg gcccttatta tattctcgca ccaggcgtgg caatgcctca tgcgcgaccg
      241 gaatgtgggg cgcttaaaac cgggatgtct ttgacattac ttgaacaagg tgtttatttt
      301 ccggggaatg acgaaccaat taaattactc atcggactct ctgctgccga tgccgattcg
      361 cacattggcg ctattcaggc gttaagtgaa ttactgtgcg aagaagaaat actcgaacaa
      421 ctcttaacag catcatcaga aaaacaatta gcggacatta tcagccgcgg ataattactc
      481 tctcctctac aggataatat tatggaaaac aagtctgctc gtgcaaaggt ccaggctttt
      541 gggggctttt tgactgcaat ggtcatcccc aatattggtg cttttattgc ctggggcttc
      601 ataactgcgt tatttattcc caccggttgg ctgccgaatg aacatttcgc caaaattgtc
      661 ggcccgatga ttacctattt attgcccgtg atgattggtt ctactggtgg tcatctggtc
      721 ggcggtaaac gcggggcggt catgggcgga ataggtacta ttggtgtgat cgttggcgca
      781 gagatcccga tgttccttgg ctcaatgatc atggggccgc tcggtgggtt ggtcataaaa
      841 tatgtcgata aggcactgga aaaacgcata cctgccggtt ttgagatggt tatcaataac
      901 ttctcattag gtatcgcggg gatgctcctt tgtctgctgg gttttgaagt gatcggcccg
      961 gcggtgttaa ttgccaatac tttcgtcaaa gagtgtatcg aggcgctggt acatgcgggt
     1021 tatctgcctc tgctgtcagt catcaatgaa ccggcgaaag tgcttttcct taataatgcg
     1081 atcgatcagg gcgtctatta tccgctggga atgcaacagg cttcggttaa cggtaaatcc
     1141 atcttcttta tggtggcctc taacccaggt ccgggcctgg ggctgctgct ggcgtttacc
     1201 ttgtttggta aagggatgag taaacgttct gcgcccgggg cgatgattat tcacttcctc
     1261 ggtgggatcc acgaactgta tttcccatat gtgctgatga agccgctgac tattattgcc
     1321 atgattgcgg gcggtatgtc tggtacctgg atgtttaact tactggacgg tggtctggtg
     1381 gctggcccaa gtccggggtc tatctttgct tacctggcac tgacgccgaa aggctcgttc
     1441 ctggcgacaa ttgccggtgt tacggtaggt accctggtgt cctttgctat tacttcgctg
     1501 atactgaaga tggaaaaaac ggtggaaacg gagagcgaag atgagtttgc tcagtcagcc
     1561 aatgcggtta aggcgatgaa acaagagggt gcattctcgt taagcagggt taagcgtatc
     1621 gcctttgttt gcgatgcggg gatgggctcc agtgcgatgg gcgcgaccac cttccgtaaa
     1681 cgcctggaaa aagcggggct ggcaatagaa gtaaaacatt acgcgataga aaacgtgcct
     1741 gcggatgccg atatcgtcgt tactcatgcc agtctggaag ggcgcgtgaa acgtgttacg
     1801 gataaaccgc tgatattgat taataactat attggcgatc caaaactcga cactttattt
     1861 aatcaattaa ccgccgaaca taaacactga ttacagaggt aaaaatgaaa accaaagttg
     1921 ctgctattta tggcaagcgg gatgtccgtc tgcgcgaatt tgaactgcca gaaattaccg
     1981 ataatgaact gttagtgagt gtaatttctg acagcgtctg tttatcgacc tggaaagcgg
     2041 cgttactcgg tagtgaacat aaacgcgtac ccgacgattt agaaaatcat ccggtcatta
     2101 ccgggcatga atgtgccggg gttattgtcg aagtgggtaa aaatctcact ggcaaatata
     2161 aaaaaggcca gcgttttgta ttgcaaccgg cgatggggtt accaagcgga tattcagcag
     2221 gctacagcta cgaatatttt ggcggtaacg ccacctatat gattattccc taaatcgcca
     2281 ttaatttggg ctgcgtatta ccgtatcacg gctcttattt tgctgcggcg tcgctggcag
     2341 agcctatgtg ctgcattatt ggtgcttatc atgccaatta tcacaccacg caatatgttt
     2401 atgagcatcg catgggcgtc aaacctggcg gcaatattgc actgctggcg tgtgcgggtc
     2461 cgatgggcat tggcgctatc gattacgcca ttaacggtgg catacaaccg tcacgggtgg
     2521 tggtggtcga tatcgacgac aaacgcctgg cgcaggtgca gaagctgctg ccggtggatc
     2581 tggcggccag taaaggcatt gagctggtgt atgtgaatac caaagggatg agcgatccgg
     2641 tccagacgct gcgggcgctg acaggagatg tcgggttcga tgacattttt gtttatgcgg
     2701 cggtgcctgc tgtcgttgag atggctgacg aattactggc ggaagatggc tgtctgaact
     2761 tctttgccgg gccgacggat aaaaacttca aagtgccgtt taatttctac aacgtccatt
     2821 acaacagcac gcacgtagtc ggtacatccg gcggttcaac ggacgacatg aaagaggcga
     2881 ttgcgttaag cgccacgggg cagttgcagc cgtcctttat ggtcacgcat atcggtgggc
     2941 tggatgcggt gccagatacc gtgctcaatc tgccggatat ccctggcggt aaaaaactca
     3001 tttataacgg cgtgaccatg ccgctcactg ccattgccga ttttgccgaa aaaggcaaaa
     3061 ccgatccgct gtttaaagag ttggcgcggc tggttgagga aacgcacggc atctggaatg
     3121 aacaggccga gaaatatctg ctggcacaat ttggcgttga tatcggggag gccgcgcaat
     3181 gatgtccctg gcgtggccat tatttcgcgt tacggaacag gcagcgttgg ctgcctggcc
     3241 gcaaaccgga tgtggcgaca aaaataaaat tgatggcctg gcggtcaccg cgatgcgcca
     3301 ggcattaaac gacgtcgctt ttcgtggacg agtggttatc ggcgaaggag aaattgacca
     3361 tgcgccgatg ctgtggattg gcgaagaggt gggcaaaggc gatgggccag aagtcgatat
     3421 cgcggttgac ccgattgaag gcacgcggat ggtagcgatg gggcagagca atgcgctggc
     3481 ggtcatggct ttcgcccctc gagatagcct gttacatgcg cccgatatgt atatgaaaaa
     3541 gctggttgtt aatcgactgg ctgccggggc gattgatctg tctttgcctc tggcggacaa
     3601 cttgcgtaac gtggcgcggg cgttaggtaa accgctggac aagctgcgca tggttacgct
     3661 ggataaaccg cgcctgagtg ccgcgattga agaagcaaca cagctgggcg tgaaggtttt
     3721 cgctctgccg gatggcgatg tcgccgccag cgtgctgacc tgctggcagg ataatcctta
     3781 cgacgtgatg tacaccatcg gcggtgcgcc agaaggcgtg atttctgcct gtgccgttaa
     3841 agcattaggt ggggatatgc aggccgagct gattgacttt tgtcaggcta aaggggatta
     3901 cacggaaaat cggcagattg cagagcagga gcgcaagcgt tgtaaggcaa tgggcgttga
     3961 cgttaaccgt gtgtactcgc tcgatgaact ggtgaggggg aacgatatcc tctttagcgc
     4021 tacgggcgtg acgggcggcg agttggtgaa cggtatccaa cagacggcga atggggtgcg
     4081 gacgcagaca ttactgatcg gcggcgcgga ccaaacgtgt aatataatag actccctgca
     4141 ttgatggtga tctatcggaa gaccaatggc gacgctgaca gaagatgatg tgcttgagca
     4201 actggatgca caggacaatt tattttcatt tatgaaaact gcgcattcta ttttgctcca
     4261 ggggatacgc cagtttctgc cgtcgctgtt tgtcgataac gatgaagaga tcgtcgaata
     4321 tgcagtgaag ccgttactcg cccaaagcgg cccgcttgac gatattgatg ttgcgctgcg
     4381 tttgatttat gcgctgggga aaatggataa atggctgtat gccgatatca cgcacttttc
     4441 ccagtactgg cattacctga acgaacagga tgaaacgccc ggatttgccg atgacatgac
     4501 ctgggatttt atcagcaacg tcaatagcat aacctgcaat gcgacgctct atgacgcgtt
     4561 aaaagcgatg aagttcgccg atttcgccgt ctggtcagag gcgcgtttta gcggaatggt
     4621 caaaacggcg ctgacgctgg cagtaacgac caccttaaag gaattaacgc cgtgaaaatt
     4681 gaattaacgg tgaatgggct gaacgtgcaa gcgcagtacc atgatgaaga gatagaacgc
     4741 gtgcataaac cgttgctgcg tatgctggcg gccttacaga cggtaagtcc gcagcgacgt
     4801 acggtggttt ttctttgcgc tccgccgggg acgggtaaat ctaccttaac taccttctgg
     4861 gaatatctcg cgcaacaaga ccctgaatta cccgctatcc agacgctccc gatggatggt
     4921 tttcaccatt acaatagctg gctggatgcg catcaattgc gccccttcaa aggcgcacca
     4981 gagacatttg acgttgcgaa actggcggaa aatctgtgcc aggtagtgga aggggattgt
     5041 acgtggccgc agtatgatcg acaaaagcat gatcctgttg aagatgcgtt gcacgttacc
     5101 gcaccactcg tcatcgtcga aggaaactgg ttgttgctgg acgacgagaa gtggtgccag
     5161 cttgcgcaat tttgtgattt ctcgatcttt atcaacgcgc cagctactgc gttgcgggaa
     5221 cgactggtag ggcgcaagct ggcgggaggg gtatcgctgg cagacgcaga agctttttat
     5281 gaccgtaccg acgggccgaa tgttcgccgg gtgctggaag agagtctgcc tgcaaatttg
     5341 accttaatga tgaccgctac gggtgaatat cgtctggtgg attaacgttc ctgattagac
     5401 catcttccag tcgcaccaca cgagagaaat gacgccgggt aaatgcggcg tcatgactga
     5461 ttgctacgac gctcgtgcct cgctggccgc acttctccag ccagctttca aacacgctca
     5521 accaattttc gtcaaaatcc cgactcggct cgtccagcag caatagtggc ggagagagtg
     5581 cttccaggca ggcgacggcg accatccgtc gctgcgcact atgcagatcc aacgggtgcg
     5641 agttggcagt gtctgctaac tgacaacatt gcagggcagc atgggtacgt tgagtgattt
     5701 catccgccgg gcatttttgc agtttcaaac caaatgcgat ctcgtcagcg acggtgctgt
     5761 gaaaaagttg gttttccgct tcctgaaaca gtacgccaac ctttgccgcg cgctggcggt
     5821 ttttcaggtt tttcatcgcc tgttgttgca gcataaccgt accggctgtg ggcgtaagaa
     5881 gacctgccat tactcgtaac agcgtcgatt ttcccgcgcc attgtcgccg gtcagcgcca
     5941 accattcacc ttgtttgagt tgcagagaaa tgtcgcaaag acaatccgtt gccgcgccag
     6001 gccagcgata agatatctta tttaacgtta acatagtggc tggagcgctc cgttttgcaa
     6061 ttgccacgct tgctggcagt agttcaggaa aggtgtgcga tgacgttcaa aaagaataat
     6121 cagcgaacca cgttcaaacg cccagtgttg taagcgctgc aacagcattt cgctggcctg
     6181 cggagtcagg cggctaaagg cttcatcaag aattaagagc tttggctgca tcgcgatggc
     6241 gcaggcgatg accacgcgct gtgtctctcc accggagagt gttgccgggt gacgatggcg
     6301 caacggctgg cactcggtca gagtcagggc ggcatcaatc cgcgccataa tctctgcttc
     6361 agcaagacac agattttctg gcccaaaggc aacttcttct tccacgctaa aggtacagcc
     6421 ggagagctgc aaatagggcg attgctggac gagctggatg gtggcggatt gttcattaag
     6481 cgggagatgc ccaatgggcg taccgaggag cgtccccgtg cctgttatct ctcctggcag
     6541 aaagtccgga taccagcccg ccatcagctg tgccagcgta cttttgccac tgccgttgtc
     6601 accaaagatg gcgaccatcc ccggtttgac atagtgaaag tcataacaga acggaggatg
     6661 cgttgagtgt gtcgggcaat aacggaactg ttctaacgta accatatcca cgctccgaat
     6721 tctgtcagca tcagcaagat catggtgtaa cgcgctacgc gttgtagggt gctatcggct
     6781 ggtgcccaca acgttgttcg tcggttgtgg atccgaaatg cccgcatatc cagcgccgca
     6841 ccacggacag ccagatcgtt aagcgcattg tgtgttagcg gaataattaa cgcgggcatc
     6901 gcacgtaaac gctgatacca gccctcatcc agcggcacgc cgcgggcacg ctgcgcttca
     6961 tgaataatcg ctaattgccg ttttaactgc tcaacgacca ataacggccc ggcaaacaga
     7021 taagcgacgc ctggcggcag gcgagaagca aacagagcgc gaataaaaca ttgcacgggg
     7081 acgtactgca tccacaactg tgaggttgaa acaatcgcca gaatccgcaa ccagagcgta
     7141 atagcatgcg accaccgttc tggcgaacgc ggcgtgccgc ttagccattc tgttagccag
     7201 ccaccgtgta ccagccacag cccggctccc agcgagaaca tcagccagac gacatatttt
     7261 gcccgccgcc gcgtggcctt tagcgcaata aggcaaaaaa aggtcgccgc gctatagatc
     7321 ggcaaaatgg tctgcgcggg caggataagc gtggtgcagg ccgccaatgc ccatagtgtt
     7381 aatgaggtaa atggatgcat ttagcgcacg gcagccattg ccggaaaatg gcgcgtggtg
     7441 cgaatcggca gctggcgcag taaaagccag acaatcaccg ctgtaagaat tttatcgacc
     7501 aggttcgcgc ctatcaccgt aatcgccacc gactccacca ggttttgccc catagagtgc
     7561 atccaggcaa cgaacaaatc ggctccgcta ccggtgacac cgccgaacag cgcagtacgc
     7621 agcggtacag cgacgaccgt taccgcaagc gtaataatca cgccgctgac aacgactttt
     7681 ggcagggtgc gaaaccaacc cgctcgcgcc agccaaccgg cgaccaggcc gatgaccatc
     7741 gcgacggggg caaatgcggc ggcgatcggg tcggtcagca atccccagag taaattggtc
     7801 agtaagccgg ttagcatccc gatcaccgga ccaagcagta ccgcgctaat aagggtgccg
     7861 atggagtcga gaaatatagg taatttgacc atgctggcca gttgcccgcc gatcatattg
     7921 atggcgatag atataacgat caacaccagt gcctgactgg aaaagtggct acgcgccata
     7981 agaaaacctc tctgaaagta gttatttaac gggggaaata tgcaactcgt cgtaaccaga
     8041 aagcgcgcaa ggttggcggc taaacgccag ttcctcaagg ctaccgatca ctagttcaag
     8101 cgtggtccgc acggtacagc cgggcatcat atgaccgcca agcatcgtgc cgtgtggatc
     8161 ggaaacgcag agatgaagat gctcaccact ctgttccagc gtgccgttta gtgagatcac
     8221 ttcgaatgta ccgttcagga gcgtggtgcc ttcttgcccg gcatagcgca aggcgacgtc
     8281 agtcaggcta ccggtacagc ctgcaatcca ggcggcatga agttgctgct gctgtgcaaa
     8341 agcgtgtagc tgggaaaaaa cttcctgccc ggggagcagg cgcagtgcgt aaaagcgtgc
     8401 ggaggaagaa tatgggtagg gcaatgtcat cattggagta ctccgaaact cggacgtatt
     8461 gcggataata tcgacgctgc atcagtcata ttctgatata agtcagggtt tgtgacggca
     8521 caacgaaaag attttgctac gcttattcgt gattctgcaa agtggaataa attccacagg
     8581 ggcggggctg aaaacgtgac gtaagtcaga tatttgtttt ctttgactgg acaatcattc
     8641 cttttattcc acgtttcgct tatcctagct gaagcgtttc agtcgattaa atgttcgaca
     8701 attaaccaat cagtcgcagt ttgcgacagg taaggtttcc ccggacgatt tgctggatta
     8761 ctctgtctgg caaatttgct cagggaaaac cttgcaggag atctatgacc gtacgcgtag
     8821 cgataaatgg cttcggtcgc atcgggcgta atgtggttcg tgctttgtat gaatcaggac
     8881 gccgtgcgga aattaccgtg gtggcaatca acgaactggc ggatgctgcg ggcatggcgc
     8941 atttgttgaa atatgacacc agtcatggcc gttttgcatg ggaagtacga caggaacgcg
     9001 atcaactttt tgttggtgat gacgccatcc gcgtattgca tgaacgttca ctgcaatcgc
     9061 tcccctggcg tgaacttggc gttgatgtag tcctcgactg caccggcgta tatggctccc
     9121 gcgagcatgg cgaagcgcat attgccgccg gggccaaaaa agtgctcttt tcacatcctg
     9181 gcagtaacga tctcgacgcg accgttgttt acggcgtcaa tcaggatcaa cttcgtgcgg
     9241 aacaccgcat cgtttctaac gcttcctgta ccacgaattg cataattccc gtcatcaaat
     9301 tgttagatga tgcgtacggt attgagtccg gcactgtgac cacaattcac tccgccatgc
     9361 acgatcaaca ggttattgat gcataccatc ctgacctgcg tcgcacccgg gcagccagcc
     9421 agtcgatcat tccggtcgat actaaactgg ccgccggtat cacacgattt tttccgcaat
     9481 ttaacgatcg ctttgaagcg attgcggtac gtgtgccaac cataaatgtg acggcaatcg
     9541 atttaagcgt gacggtgaag aaacctgtaa aagccaatga agtcaacctg ttgctgcaaa
     9601 aagcagcaca aggtgcattt catggtatag ttgactatac ggaattgccg ttggtctctg
     9661 tagattttaa ccacgatccg cacagtgcca ttgtcgatgg cacccaaacc cgggtcagtg
     9721 gcgcacacct gatcaaaacg ttggtctggt gcgataacga atggggcttt gctaaccgaa
     9781 tgctcgacac gacgttagct atggctactg ttgctttcag gtaagacgca agcagcgtct
     9841 gcaaaacttt tagaatcaac gagaggattc accatgtctg taattaagat gaccgatctg
     9901 gatcttgctg ggaaacgtgt atttatccgt gcggatctga acgtaccagt aaaagacggg
     9961 aaagtaacca gcgacgcgcg tatccgtgct tctctgccga ccatcgaact ggccctgaaa
    10021 caaggcgcaa aagtgatggt aacttcccac ctgggtcgtc ctaccgaagg cgagtacaac
    10081 gaagaattct ctctgctgcc ggttgttaac tacctgaaag acaaactgtc taacccggtt
    10141 cgcctggtta aagattacct cgacggcgtt gacgttgctg aaggtgaact ggttgttctg
    10201 gaaaacgttc gcttcaacaa aggcgagaag aaagacgacg aaaccctgtc caaaaaatac
    10261 gctgcactgt gtgacgtgtt cgtaatggac gcattcggta ctgctcaccg cgcgcaggct
    10321 tctactcacg gtatcggtaa attcgctgac gttgcgtgcg caggcccgct gctggcagct
    10381 gaactggacg cgctgggtaa agcactgaaa gaacctgctc gcccgatggt ggctatcgtt
    10441 ggtggttcta aagtatctac caaactgacc gttctggact ccttgtctaa aatcgctgac
    10501 cagctgattg ttggtggtgg tatcgctaac acctttatcg cggcacaagg ccacgatgtg
    10561 ggtaaatccc tgtacgaagc tgacctggtt gacgaagcta aacgtctgct gaccacctgc
    10621 aacatcccgg ttccgtctga tgttcgcgta gcaaccgagt tctctgaaac tgcaccggct
    10681 accctgaaat ctgttaacga tgtgaaagct gacgagcaga tcctggatat cggtgatgct
    10741 tccgctcagg aactggctga aatcctgaag aatgcgaaaa ccattctgtg gaacggtccg
    10801 gttggcgtgt tcgaattccc gaacttccgc aaaggtactg aaatcgtggc taacgctatc
    10861 gcagacagcg aagcgttctc catcgctggc ggcggcgaca ctctggcagc aattgacctg
    10921 ttcggcattg ctgacaaaat ctcctacatc tccactggcg gcggcgcatt cctcgaattc
    10981 gtggaaggta aagtactgcc tgcagtagcg atgctcgaag agcgcgctaa gaagtaaaaa
    11041 atcacagggc agggaaacct gcccttgttt cagcgcgctt ttagagcacg cattttttca
    11101 tactctaaat aattcgagtt gcaggaaggc gacaagagag tgaatcccca ggagcttaca
    11161 taagtaagtg actggggtga gcgaacgcag acgcagcaca tgcaacttga agtatgacga
    11221 gtataaggcc cgacgataca ggacaagaga catgtctaag atttttgatt tcgtaaaacc
    11281 tggcgtaatc actggtgatg acgtacagaa agttttccag gtagcaaaag aaaacaactt
    11341 cgcactgcca gcagtaaact gcgtcggtac tgactccatc aacgccgtac tggaaaccgc
    11401 tgctaaagtt aaagcgccgg ttatcgttca gttctccaac ggtggtgctt cttttatcgc
    11461 tggtaaaggc gtgaaatctg acgttccgca gggcgctgct atcctgggcg cgatctctgg
    11521 tgcgcatcac gttcaccaga tggctgaaca ttatggtgtt ccggttatcc tgcacactga
    11581 ccactgcgcg aagaaactgc tgccgtggat cgacggtctg ctggacgcgg gtgaaaaaca
    11641 cttcgctgct accggtaagc cgctgttctc ttctcacatg atcgacctgt ctgaagaatc
    11701 tctgcaagag aacatcgaaa tctgctctaa atacctggag cgtatgtcca aaatcggcat
    11761 gactctggaa atcgaactgg gttgcaccgg tggtgaagaa gacggcgtgg acaacagcca
    11821 catggacgct tctgcactgt acacccagcc ggaagacgtt gattacgcat acaccgagct
    11881 gagcaaaatc agcccgcgct tcaccatcgc agcgtccttc ggtaacgtac acggtgttta
    11941 caagccgggt aacgtggttc tgactccgac catcctgcgt gattctcagg aatatgtttc
    12001 taagaaacac aacctgccgc acaacagcct gaacttcgta ttccacggtg gctccggttc
    12061 tactgctcag gaaatcaaag attccgtaag ctacggcgta gtgaaaatga acatcgatac
    12121 cgatacccaa tgggcaacct gggaaggcgt tctgaactac tacaaagcga acgaagctta
    12181 tctgcagggg cagctgggta acccgaaagg cgaagatcag ccgaacaaga aatactacga
    12241 tccgcgcgta tggctgcgtg ccggtcagac ttcgatgatt gctcgtctgg aaaaagcatt
    12301 ccaggaactg aacgcgatcg acgttctgta agatattcct ttctgcttat ctcaaggccc
    12361 gctctgcggg tctttttttc gccaaaagca aaattgcctg atgcgctacg cttatcaggc
    12421 ctacacgtat cctgcaatat attgaatttg caggtttttg taggccggat aaggcgttca
    12481 cgccgcatcc ggcatgacaa agcgcacatt gatatctgtc tgagccccgc tctgcgggga
    12541 ttttttcgcc aaaagcaagg tgattcagat gagaaatctg tgatctattt ggcaaaatta
    12601 tgctttattg tttacccttg tcagactgcc cgtcataagg cggcggagtg tatttctcca
    12661 ttttgagtca gttgaaaagg aatattgaat ggaagatttg aatgttgtcg atagcataaa
    12721 cggcgcggga agctggctgg tagctaacca ggcgctgctg ctaagttatg cagtaaacat
    12781 cgtggcggca ctcgcgatca tcatcgttgg tttgattatc gcgcggatga tttccaacgc
    12841 ggtgaatcgc ctgatgatct cccgtaaaat cgatgccact gttgctgatt ttctttctgc
    12901 attagtccgt tacggtatta tcgcctttac gctaatcgct gccctgggac gcgtgggtgt
    12961 acaaacagcg tcagtcattg ctgtactcgg tgccgcaggc ttagctgttg gtctggcttt
    13021 gcaggggtca ctttctaacc tggccgctgg cgtgttactt gtcatgttcc gcccgttccg
    13081 tgccggagaa tatgttgacc tcggcggcgt agccggtact gtgctgagtg tgcagatttt
    13141 ctccaccacc atgcgtactg cagacggtaa aattatcgtt attccgaacg gtaaaattat
    13201 tgccggaaat attattaact tctcccgcga gccagttcgc cgtaacgaat ttattattgg
    13261 cgtggcgtat gattccgata tcgatcaggt taagcagatc ctgaccaata ttatccagtc
    13321 tgaagatcgc attttgaaag atcgcgaaat gactgtgcgc ctgaacgaac ttggtgcatc
    13381 gtcgattaat ttcgtggtcc gcgtctggag caacagcggc gatctgcaaa acgtgtactg
    13441 ggatgtgctg gagcgtatta aacgtgaatt tgatgccgcc ggtatcagct tcccgtaccc
    13501 gcaaatggat gtgaacttta agcgggtgaa agaagacaaa gctgcgtaat caacgctgag
    13561 gccagataat actctctggc ctctctctta ttagttattc tgattgccaa ttaatattat
    13621 caatttccgc taataacaat cccgcgatat agtctctgca tcagatactt aattcggaat
    13681 atccaacgtg ttttcttatt actttcaagg tcttgctctt agggcagcta tgattctgcc
    13741 gctcggccca caaaatgctt ttgtgatgaa tcagggcatt cgtcgtcagt accacattat
    13801 gattgcctta ctttgcgcta tcagcgattt ggtcctgatt tgcgcgggga tttttggtgg
    13861 tagcgcgtta ttgatgcagt cgccgtggtt gctggcgctg gtcacctggg gcggcgtagt
    13921 cttcttgctg tggtatggtt ttggcgcttt taaaacagcg atgagtagta atattgagtt
    13981 agctagtgcc gaagtcctga agcaaggcag atggaaaatt atcgccacca tgttggcagt
    14041 gacttggctg aatccgcatg tttacctaga tacttttgtt gtactgggca gccttggcgg
    14101 gcagcttgac gtcgaaccaa aacgttggtt tgcgctcggg acaattagcg cttctttcct
    14161 gtggttcttt ggtctggcta ttctcgcagc ctggctggca ccacgcctgc gcacggcaaa
    14221 atcacagcgc attatcaatc tggtagtggg atgtgtcatg tggtttatag ccttgcagct
    14281 ggcgagagac ggtattgctc atgcacaagc cttgttcagt taggcgctat ctgatggaaa
    14341 aataaaacag aggcgctaag cttgcctcca gaggtcctga attttgggca agtcaattaa
    14401 cacggagaga ctaacgtgaa gttcaaagtt atcgccctgg cggcattaat gggtattagc
    14461 gggatggcag cgcaggctaa cgaattgccg gatggaccgc atattgtcac ctccggtacg
    14521 gcaagtgtgg atgcggtgcc agacattgcc actcttgcga ttgaagttaa cgtggccgcg
    14581 aaggatgccg ctacagccaa gaaacaggca gatgagcgcg tcgcacaata catttccttc
    14641 cttgaactca atcagatcgc gaaaaaagat atcagctcag cgaatttacg cacccagcca
    14701 gattatgatt atcaggatgg taaaagtatc ctgaaaggct accgcgctgt gagaacggtg
    14761 gaagtcacgc tccgtcagtt agacaaactg aattccttgc tggatggcgc gctgaaggcg
    14821 ggtcttaacg aaattcgttc agtgtcgctg ggcgtggcgc agccggatgc ctataaagac
    14881 aaagcgcgta aggcagcgat tgataacgcg attcatcagg cgcaggaact ggcgaacggc
    14941 tttcatcgta aactggggcc ggtatatagc gtgcgctacc atgtttccaa ctatcagccc
    15001 agcccaatgg tgcggatgat gaaagccgat gccgcgccgg tgtctgccta ggaaacttac
    15061 gagcaggccg ctattcagtt tgatgatcag gtcgatgtgg tcttccagtt agaacctgtg
    15121 gatcaacaac ccgctaaaac acctgcagca caataatact tgtagcccga cgtattcgtc
    15181 gggctaatgc ctttacccga tattgcctcc tttttctgta attttctgta aaaccatgac
    15241 gtaatcacct atctttgctc gcggtttcat caaggatagg ctgactgaaa cggttaagcg
    15301 gacaaggatg cggggagggg ggatggacat ttttatttcg aaaaagatgc gcaatttcat
    15361 tttattagcg caaaccaata atattgcccg ggcggcagaa aaaatccata tgacggcttc
    15421 gccatttggt aaaagcattg ccgcgctgga agaacaaatt ggctatacgc tatttacccg
    15481 caaagataat aatatcagcc tcaataaagc cggtcaggaa ctgtatcaaa aactgttccc
    15541 ggtttatcaa cgactttctg cgattgataa tgaaatccat aactcagggc gtcgttcacg
    15601 agaaattgtg attggcatag ataatactta tccgacaatt attttcgatc agttgattag
    15661 tctcggagat aagtatgaag gcgtgaccgc ccagccagtt gagttcagcg aaaacggcgt
    15721 tatcgataat ctttttgacc gtcagttaga ttttattatc tccccacagc atgtatctgc
    15781 acgagttcag gaactggaaa acctgactat cagtgaatta ccaccgctgc gattgggctt
    15841 tctcgtttct cgccgttatg aagggcgtca agagcaagag ctgcttcagg agctgccctg
    15901 gctacaaatg cgctttcaaa accgggctaa cttcgaagca atgattgatg cgaatatgcg
    15961 accttgtggg atcaatccca ccattattta tcgtccctac agttttatgg ccaaaatcag
    16021 cgccgtggag cgcggacatt ttctgacggt gatcccgcat tttgcctggc gactggtgaa
    16081 tccggcaacg ctgaaatatt ttgatgctcc gcacaagccg atgtatatgc aggaatatct
    16141 ttattcaatc agaaatcatc gttataccgc cacgatgctt cagcatattg ctgaagatcg
    16201 tgacgggacg agtcattaac ccagcatcga gccggttgca attaaattac ggtgtaagtc
    16261 gaagacgtgg ctaagatcgt ggtgaatatg tccgccaggc gcattttcca gatagcgatg
    16321 cagataatcc tgatacatag gatgtgcaca attatcaatg atagtgcggg cgcgttgaag
    16381 cggggaaaga ccgcgcagat cggcgatccc ttgttcggtg atgatcactt tgacgctgtg
    16441 ttcgctgtga tcaacatggc tgcacattgg cacgacggtt gagatcttgc cttctttagc
    16501 aatcgacggg gccataaaga tcgacagata cgcgttgcgt tcaaaatcac cgctgccacc
    16561 gatgccgttc atcagatcga ccccggctac gtgcgttgag ttggcatgcc cgtaaatatc
    16621 aaactccagg ccgacgttca gagcgatgac gcccagacga cggatgattt ccgggttatt
    16681 ggaaatctcc tgcggacgca acacaatgcg gctggcaaag taatccatat tgtcgtaaat
    16741 cttgcgcagg gaatcggccg agattgtcag gctggaggcg ctggccccgc tgattttgcc
    16801 ggtttccagt aaatgcacca ccgattcctg tagcacttcc gaatacatca taaacggagg
    16861 aatttccggg ttttccccca gacgcgccat taccgcatta ttgatattgc ccacgccact
    16921 ttgcagcggc agaaattccg gcggaatacg cccatgcgcc atttcctgca acaagaacgt
    16981 gaccacgtta tcggcaatct gctggcacat gggattttgc ttatccagca tattaccggc
    17041 gtcgggcaag ttggtttcca cgacggcgac aatctttttc ggatcgattt gcacatagcg
    17101 ggtaccgacg cgatccattg catggaagat cgacacgcta ttgcgccgcg gtggcgcgcc
    17161 aggaatcaca atatccgcca gttctgcaac gcgcggatcg tgatagtgat tgagttcaat
    17221 gatcactttc ttcgcccgca gcagccaggt cggcgcatta ccgatcccgc tggttaacca
    17281 gactcgacca tccggtgcca gtgccgatgc ttcaatgacg gcaacatcaa tgtcgccgaa
    17341 gaaaccgtaa ttgaccattt gcgccacttc gctcaaatgc aggtcaacga aactcaccgc
    17401 gccctgattg atctttttac gtaaaccgga cgatgtttga tatggcgcac gccaggaaac
    17461 agcatcggcg tcagaaagta catcgtcagc ggcggcgctg attgacgcac ccgtcagaag
    17521 gcgaatttga tacggctttt tggcctcatg ctgttcgtta gctctgcggg caatcgcggt
    17581 gggtagggct ttcggcgaac ccgccggggt aaagccgcta aatgccacca tgtcgttatg
    17641 ctggataatt tctgccgctt cattggcggt catccttgtc cactgagttt ccatttttac
    17701 tccttcgttc gcagggatta atgaccaacg aaattaggtt tacgtttttc gaggaaagcg
    17761 ttcatccctt cctggtaatc ttcgctgtca tacaccgcgc ggcgcatccc ctgaatacgt
    17821 tcaaattcat cggagttcat ggtgtgtgct tcgcccagta cacgcagctc ttctttgata
    17881 acggcaatgg ctaacggcgc tttctcagag atgtggtgcg ccatttgtaa ggtgaaatct
    17941 tccagttctt ccacttccac aacatggttg aggatgccga cagccagcgc gcgctgggcg
    18001 gtgattggcg aagcggtaaa aatcagctct ttgacaatgt ggaagcccgc gtcgcgggtc
    18061 aggttgtgaa tgccgaccag gttatacggg acgccgaggt ttacaggcgt cattgagaag
    18121 gttgaggtac tggcggcgat gatcagatcg gaactcatga tcatttcaaa tgcgccaccc
    18181 caaacactac cttccaccat cgaaatgatc ggtttcggga atttttggat catgcgggtg
    18241 atttgacgca atggatcatc ataggagagc ggatcgcgac cgccagacgg cagttcgtga
    18301 atatcgtggc ctgcggagaa gactttggat ccactcggcg cgcgcaaaat gatacagcga
    18361 atttccggcc ggttgagatc gcttaatgcc tgcataagat catcaataaa gactttactt
    18421 aaggcattaa gtttgcggcc atagttaaac tcaatgaccg ccactttgtt gatagtgaca
    18481 acgttaacat actgataaga cataaaaatt cctttaatca aaatattgcg tctggataaa
    18541 ttcactgagc tgccgcaggc cggtgcgcgg tgagagcgta ttgtttttga ccgctaaaag
    18601 cgtctggcgg taatagcgat cgaaatcttc attcgcgaac aggtgattca gtacttcttc
    18661 ttcggtctgc ttacgcagcc attccaccga ttgttgttgc cgcacttgtt gtaaacgacc
    18721 actggcagtt agcgcggttt tgaagttgat gatggcgtgc cagatctcat cgattccacg
    18781 tttttccagt gcgctacaag tcagaacccg tggctgccat tcgtcgtatt tacgtcgcag
    18841 aatatgcagg gcactctcgt acatatgccg ggcaatggcg acattggtat ggttatcgcc
    18901 atcgtctttg ttgataacga gcagatcagc cacttccatc agcccttttt taatgccctg
    18961 cagatcatcg ccgccaccgg caatttgcaa cgagataaaa cagtccacca tgcgggcgac
    19021 ttccgtttcc gactgcccga cgccaaccgt ttcgacaatc actacgtcat aacccgccgc
    19081 ttcgcataac agcattaatt cccgcgctcg ctgactggca ccgcccagat gaccggagga
    19141 tggtaccggg cgaataaacg ccgcttcggc acgcgccagg tcattcatgc gggttttatc
    19201 cccgagaatg ctaccgccag tgaccgggct gctgggatcg accgcaataa ccgcgacctt
    19261 taatccctct cgaatcaaca acatgccaaa ggcctcaaga aaggtacttt tccccgcgcc
    19321 gggggtgccg gtaacgccca gtcgcagggt gttaccgcag tacggcataa tggtatcaag
    19381 cagctgcgta cttagtgcct gatgacgcgg gtgacggctt tccaccagcg tcatggcctg
    19441 ggcgagtgtg gcacgctcac cctgacgtaa gcggcgaata ctttctgcca gcgtggcttc
    19501 attaatcatg atgctggctt atcagattca gtacgtcgcg cacactgtcg agcataggtg
    19561 tacctggacc ataaatcgcc gccacgccgc gctcttgcag gaaggcgtaa tcctgcggcg
    19621 gaatgacgcc acccgcgacc acgcagatat cttcgcgtcc ccattttttc agcgcttcga
    19681 ccagttccgg gatcagcgtt ttatgaccgg cagccagtga ggatgcgccc actacgtgaa
    19741 cgtcgttttc tacggccagg cgggcgatct cttcaggtgt agagaacatc gggcttaaat
    19801 ctacgtcgaa accgagatcg gaataggcgc tggcgatcac tttcgcgccg cgatcgtgtc
    19861 catcctggcc catcttagcg atcagaatgc gcgggcgacg accattgtcg gcaaggaact
    19921 gctccgtttg cgcaacaatg gcatcgaact cggaggccga tttctccgac tgatgatagc
    19981 tttgcgcaat tacgccggta acacactggc ttggcaccag ataacggtcg aaagcggctt
    20041 tcagcgcatc ggaaatttca cccagggtgg cgcgaacgcg agcggcatta acagcggcag
    20101 ccagcaggtt ttcgttatgc tgtgcggcgt gagtcagggc gttcaacgcg gcggttacgg
    20161 cggcatcatc acgggtggcg cgaatgcgtt ccagcgaagc aatttgctcg ttacgcacca
    20221 tcacgttgtc gatctcaagt acatcggttt cgtcttcgtg atccagtttg tacttgttga
    20281 caccaacgat gacacgcttg ccctggtcga tcagcgactg ttcgcgcgct gaggcctctt
    20341 cgatcattcg ttttggcaga cctgcttcga tcgctttcgc catgccaccg gcttcgtcga
    20401 tctgttggat aatagctctg gcttgtttga cgatttgatc ggtcagcgac tcaatgtaat
    20461 aggatccggc cagtggatcg acggtgcggc agagttctga ttcttcctgg atgatgatct
    20521 gggtgttgcg ggcaatgcgt gctgagaaat cggtaggcaa accaagcgct tcgtcaaagg
    20581 cgttggtatg cagtgactga gtaccgccca gcgtcgcagc cagcgcttca atggtggtgc
    20641 ggataacgtt gttatacgga tcctgttcag tcaggctcca gcctgaggtc tggcagtggg
    20701 tacgcagcgc cagtgatttc gggtcctgtg cgccaaatcc actgacctct tcgctccata
    20761 aataacgtgc cgcacgcaac atggcgacgt tcataaacag atccatgccg atgccgaaga
    20821 agaacgacag gcgaggagcg aagtcatcaa ttttcagtcc ggcagagatt gctgctttga
    20881 tgtactcaat cccatcagcg agcgtaaatg ctacctgctg cacgcagttg gcacccgctt
    20941 cacccatgtg gtaaccgctg atactgatgg tattaaatcg cggcatgttg ccggaacacc
    21001 aggcgatgat gtcggcgata atgcgcattg acggttttgg cgggtaaata taggtgttgc
    21061 ggcagaggta ctctttgaga atatcgtttt gaatggtgcc ggtcagttta tcaggtgtaa
    21121 caccttgctc ttctgcggcg acgatataaa acgccagtac tggtagcact gcgccattca
    21181 tggtcatcga aaccgacatt ttatccagcg ggatctggtc gaacaggact ttcatatctt
    21241 ccacggtgtc gatagcgacg cccgctttgc cgacgtcacc cgccacgcgt gggttatcgg
    21301 agtcgtagcc acggtgggtg gcaaggtcaa acgcaacgga aagacctttt tgcccggcgg
    21361 ccaggttacg gcgataaaaa gcgttggact cttttgctgt tgaaaaacca gcatactgac
    21421 ggatggtcca cggttgggcg gtatacatag tggcacgcgg gccacgaacg tagggcggca
    21481 aaccaggaag ggtacctgtc acctccagat tatcgagatc ggcttcggta tacagcggct
    21541 tgatggcgat cccttccgcg gtttgctgaa ccagcgagtc gacagttttc tcccgacggc
    21601 tcaattcctt gttggcaagc tgttgccact cctgctcgtt agacattatt tgctccgtaa
    21661 aacgcaatca aaatgagggt atttgcgctt acggtgagct ttttacgtgg gtttgtcgcc
    21721 agcaaaaaaa atgcattcag acgtcgtttt ttgccgcctg agatttgtct attttgtgat
    21781 gtgattcaag gaatacaaaa agaagtgagg acaacgcctg atatgtgcta tcaggcatta
    21841 tttgatggat taatcctgac gaaggacttt gtgaccataa tcgagtaacg catcagtgac
    21901 tttacgcatc atgcggcttt caggagcaaa gcggtgccag tagagcatcc gtcgttgaaa
    21961 tagcccaggc gttaagtcaa tcagttcacc gctggccagc tctttctcga tttgcaggtg
    22021 cgggatcata cagcaggtgg tgccctggcg agcaagttgt acgaacgctt ctgaagaatt
    22081 aacgatatgg cagggcacgc tgcctggagg cagatcgaag ttttgctgca aaaaggcctg
    22141 gtgcatatcg tcaagatggt caaacgcgac cactggcgct ttcagtaatg ccgaacgcgt
    22201 tacgccgtta gggaaatatt tttcggcaaa gggttttgag ctgacgaaca gatagtcgag
    22261 cgcaccaagt ttatcgacaa gacaactcgg cagcgcctga tgttgaatac tcaccgcgcc
    22321 gaccacttcg ccgcggcgca gacgttcctg agtgcgggtt tcatcttcta cctgcaagtt
    22381 gaggcggata ggcgaatcag ccaacacagg agccagtgca ggaagcaacc acgtcgccag
    22441 actgtcggcg ttgaccgcca gtgaaagcag cagcggagtc gaaccggttt gttcatcgcc
    22501 cagccactct tcttccagca actccacctg gcgcagcagt gccagcagtt tttgcccttg
    22561 ttccgtcggg cgcggcggta cggtacgcac caacagcggc tgcccgaaca tattttccag
    22621 ttgcttaatg cgctgtgaga cggctgattg tgtaatgcac agcttttgtg ccgcgcgctc
    22681 aaatcctcgt tcacgtatca ccgcatccag tgcctgtaat gttctgtagt ccgggcgttt
    22741 cattgctgtt gtctgctcct ggtaaatgct ttttcctgca ctatgacaca attttgtgtc
    22801 aggttgcata taccgaagcc gtcggcgggt aacatttgta ttcgtggtta aacgcgcagc
    22861 ttaaaagatc agggatttgc gctaatgcat ttcgctcagc gagttcgcgc cctggttgtg
    22921 ttgaatggcg tggcgttatt gccccaattt gcctgtaaac aggggcttgc gaacggtgaa
    22981 ctggtgcgcc tgtttgcacc gtggagcggc atacccagat tgttatatgc cttatttgcg
    23041 gggcgaaagg ggatgcctgc cattgcgcga tattttatgg atgagttaac cacgcggctt
    23101 gccaacgggg tctgaatcgc tttttttgta tataatgcgt gtgaaatttc ataccacagg
    23161 cgaaacgatc atgacgcagg atgaattgaa aaaagcagta ggatgggcgg cacttcagta
    23221 tgttcagccc ggcaccattg ttggtgtagg tacaggttcc accgccgcac actttattga
    23281 cgcgctcggt acaatgaaag gccagattga aggggccgtt tccagttcag atgcttccac
    23341 tgaaaaactg aaaagcctcg gcattcacgt ttttgatctc aacgaagtcg acagccttgg
    23401 catctacgtt gatggcgcag atgaaatcaa cggccacatg caaatgatca aaggcggcgg
    23461 cgcggcgctg acccgtgaaa aaatcattgc ttcggttgca gaaaaattta tctgtattgc
    23521 agacgcttcc aagcaggttg atattctggg taaattcccg ctgccagtag aagttatccc
    23581 gatggcacgt agtgcagtgg cgcgccagct ggtgaaactg ggcggtcgtc cggaataccg
    23641 tcagggcgtg gtgaccgata atggcaacgt gatcctcgac gtccacggca tggaaattct
    23701 tgacccgata gcgatggaaa acgccataaa tgcgattcct ggcgtggtga ctgttggctt
    23761 gtttgctaac cgtggcgcgg acgttgcgct gattggcaca cctgacggtg tcaaaaccat
    23821 tgtgaaatga tctgacgggg gaacctcccc cgttaaaaaa attctcttca ttaaatttgg
    23881 tgacatgtgt cacgctttta ccaggcaatt gtcgattgct ctaaataaat cctctaaacc
    23941 agcatattca tccaagaatt acctttgcgt gatatttcct caacatcgcg acgcaaacgt
    24001 tcatattgcc gcaatattat tttttgatat gttgaaaggc ggatgcaaat ccgcacacaa
    24061 catttcaaaa gacaggattg ggtaaatggc aaaggtatcg ctggagaaag acaagattaa
    24121 gtttctgctg gtagaaggcg tgcaccaaaa ggcgctggaa agccttcgtg cagctggtta
    24181 caccaacatc gaatttcaca aaggcgcgct ggatgatgaa caattaaaag aatccatccg
    24241 cgatgcccac ttcatcggcc tgcgatcccg tacccatctg actgaagacg tgatcaacgc
    24301 cgcagaaaaa ctggtcgcta ttggctgttt ctgtatcgga acaaaccagg ttgatctgga
    24361 tgcggcggca aagcgcggga tcccggtatt taacgcaccg ttctcaaata cgcgctctgt
    24421 tgcggagctg gtgattggcg aactgctgct gctattgcgc ggcgtgccgg aagccaatgc
    24481 taaagcgcac cgtggcgtgt ggaacaaact ggcggcgggt tcttttgaag cgcgcggcaa
    24541 aaagctgggt atcatcggct acggtcatat tggtacgcaa ttgggcattc tggctgaatc
    24601 gctgggaatg tatgtttact tttatgatat tgaaaacaaa ctgccgctgg gcaacgccac
    24661 tcaggtacag catctttctg acctgctgaa tatgagcgat gtggtgagtc tgcatgtacc
    24721 agagaatccg tccaccaaaa atatgatggg cgcgaaagag atttcgctaa tgaagcccgg
    24781 ctcgctgctg attaatgcct cgcgcggtac tgtggtggat attccggcgc tgtgtgatgc
    24841 gctggcgagc aaacatctgg cgggggcggc aatcgacgta ttcccgacgg aaccggcgac
    24901 caatagcgat ccatttacct ctccactgtg tgaattcgac aacgtccttc tgacgccaca
    24961 cattggcggt tcgactcagg aagcgcagga gaatatcggc ctggaagttg cgggtaaatt
    25021 gatcaagtat tctgacaatg gctcaacgct ctctgcggtg aacttcccgg aagtctcgct
    25081 gccactgcat ggtggtcgtc gtctgatgca catccacgaa aaccgtccgg gcgtgctaac
    25141 tgcgctgaac aaaatcttcg ccgagcaggg cgtcaacatc gccgcgcaat atctacaaac
    25201 ttccgcccag atgggttatg tagttattga tattgaagct gacgaagacg ttgccgaaaa
    25261 agcgctgcag gcaatgaaag ctattccggg taccattcgc gcccgtctgc tgtactaatt
    25321 ccccttctct gaaaattaac gggcaggtca ctgacttgcc cgttttttta tcccttctcc
    25381 acaccgttgc cgcgttgcgc attctacttt gcgagtctgc tccagaaaat aaatcaaagt
    25441 cagatcaatg cgttgtgttt atttaatggg gatttacgat ggcgggacag catggggctg
    25501 taacggtaaa gccctcaccg aagcgagggc ttaaaggaga agggttatga tgcgacttgt
    25561 catcatactg attgtactgt tactgataag tttcggagct tattaacagt cagtctcagg
    25621 ggaggagtca tcctccctta cccttactca ctaaattagg tcaaagaatc aacgatgtca
    25681 atcagggcga tgcggggtgc atcgccctga accgaacttt ttgatagcca cttcatcgcc
    25741 cacgtttact gccgtaatag atcctttttc ccaaaaatta tctctgtttt gcatggggtt
    25801 tttcgagcac gctcgaagaa aagatttaaa ggtagatcaa tgcgttgtgg ttatttaaag
    25861 agagcttacg atagctgggc agcatggggc tgtaacggta aagccctcac cgaagcgagg
    25921 gcttgaagga gaagggttat gatgcgactt gccatcatac tgattgtact gttactcata
    25981 agtttcccag cttattaaca gtcagtcaca ggggaggagc aatcctccct tacccttact
    26041 cactaaatta ggttaaagaa tcaacgatgt caatcagggc gatgcaggtg tatcgccctt
    26101 accactccca gactttcgac ggtgtaacca ccgcaggaag agggatatcc cactcttcaa
    26161 cggggagttt ttccaccaac tgacaatcat gcgcataacc caccggttgc gttttatagt
    26221 gctgccagtt ttgtaaggtc cgatcataaa aaccaccgcc cattcccagg cgctgaccgt
    26281 actcatcaaa ggcgaccagc ggtgtgatca gcacgtctaa tcgggaaagg ggtagcacgt
    26341 cacgcacatc cagttttggc tcatggattt tcaacctgtt catcaccagt tcgctttgcg
    26401 gatggtaatt caggaacagc aaattaccgg cactaaaggg atgcaaaact ggaagataaa
    26461 cgcgcttacc ggcgcgccag agttgttcta tgagtggctg ggtgtcgagt tcgccatcaa
    26521 aagagaggaa tacagcgacc gtatgtgcca tcaccaccgg gggataagtc atcatccggg
    26581 tagcggcttg ttgacccatt tcttgctgtt gttccggcgt taacgcacga cgacgttgcc
    26641 gaatcatttt gcggatttct tgtcgggata atgtcagtgg gagttctggt agttgcgtca
    26701 tggctggtgc cagataagaa gggaatctcc gagatgccgc cgcaggctgt aacccttgaa
    26761 cccttggttc aaggtgaatg tgtcgtcgca gttttaaggc ttctcggacg gaccgagcat
    26821 gctcaccaac cgcggagcgc cacattcttg tggtatgaaa tatcggctca ggggactggc
    26881 ccgcttgcga acatctcaga gaaattttgt cttcacggtt actctaccac agtaaaccga
    26941 aaagtgtcat tcaaagtttt ggttagtttt ttcggtgatg cgaccttgtt caagtaacgc
    27001 ttgttctatg gtctgctgca gcatccgaat acgctgttcc atacttgccg cgtagtcacg
    27061 agtctttgct ttttcttgcg ctaactcata gctgatattc aatgcggcaa tgaagaccaa
    27121 ctgttcagta tttgtgactc tagtgcgttc tttcagatct tgcaaccgtt ggttcagatc
    27181 gtccgctgcc tgattcaacg catccctttg gtcaggcggg cagttcacac gcagtgaacg
    27241 gccaaaaatt tggatatcga cgggttgtgc agacatgcca ccttcctgct gattgactgc
    27301 gctgccttcg tctttagacc catggtctgc gaaggggcga cactatagct accctgatga
    27361 gaagagacaa gcccttttct ggtccaccag ggcccaaagt ggtagcatat catgaatatt
    27421 cctccctttg acgacgaatg cttatgtcta tacagaacga aatgcctggt tacaacgaaa
    27481 tgaaccagta tctgaaccaa caagggacgg gtctgacccc tgctgagatg catggtttaa
    27541 tcagtgggat gatatgtggc ggtaacgatg acagctcatg gctaccgcta cttcacgacc
    27601 tgacgaacga aggcatggct ttcggtcatg agctggcaca ggcactgcgt aaaatgcact
    27661 ctgccaccag cgatgccctg caggatgacg gcttcctttt tcagctttat ctgcctgatg
    27721 gcgatgatgt cagcgttttc gatcgggctg atgcattggc aggttgggtc aatcacttcc
    27781 tgcttggtct tggcgttacg caaccgaagc tggataaagt gaccggcgaa accggtgaag
    27841 ctatcgacga tctgcgtaac attgcgcaac tgggttacga cgaagacgaa gatcaggaag
    27901 agcttgaaat gtcgcttgaa gagatcatcg aatacgttcg tgttgccgcg ctgttatgcc
    27961 acgacacctt tactcatccg caaccgaccg cgccagaagt acaaaaaccg actctacact
    28021 aaaaacaaaa aacgtaagga gagtgttatg agtgagatat cccggcaaga gtttcagcgt
    28081 cgccgtcagg ccctggtgga gcaaatgcaa cccggcagcg ccgcgctgat ttttgctgca
    28141 ccagaagtaa cacgtagcgc cgacagcgaa tacccctatc gtcagaacag tgacttctgg
    28201 tacttcaccg gctttaacga accggaagcg gtgctggtgc tgattaaaag cgatgacact
    28261 cataaccaca gcgttctgtt taaccgcgtt cgcgacctga cggcggagat ctggtttggc
    28321 cgtcgcttag gccaggatgc cgcgccagag aaactgggcg ttgaccgcgc actggcattc
    28381 agcgaaatca atcagcaact ttatcaacta cttaacggcc tggatgtggt ttaccatgcc
    28441 cagggcgaat atgcatatgc tgatgaaatc gtgaacagtg cgctggaaaa actgcgtaaa
    28501 ggttcgcggc aaaatctcac cgcaccggca acgatgatcg actggcgtcc tgttgttcat
    28561 gaaatgcgcc tgttcaaatc gccagaagag attgccgtac tccgccgcgc gggagaaatc
    28621 accgccatgg cacatacacg ggcgatggaa aaatgccgtc cgggaatgtt cgagtaccat
    28681 ctggaaggcg aaattcacca cgaatttaac cgccacggtg cgcgctatcc gtcctataac
    28741 accattgtcg gcagcggtga aaacggctgc attctgcact acaccgaaaa cgagagtgaa
    28801 ctgcgcgacg gtgacctggt gttgattgac gcgggttgtg aatacaaagg ttacgcgggc
    28861 gatattaccc gcaccttccc ggtcaacggc aaattcaccc aggcccagcg tgaaatctac
    28921 gacattgtgc tggagtctct cgaaaccagc ctgcgcctgt atcgtccggg aacttccatt
    28981 ctggaagtca ctggtgaagt ggtgcgcatc atggttagcg gcctggtaaa actcggcatc
    29041 ctgaaaggtg atgttgatga actgatcgct cagaacgccc atcgtccttt ctttatgcat
    29101 ggccttagcc actggttagg actggatgtc catgacgttg gcgtttatgg tcaggatcgc
    29161 tcgcgcattc tggaaccggg gatggtactg accgtagagc ctggtctgta tatcgcgccg
    29221 gatgcggatg tgccagaaca atatcgcggt atcggcattc gtattgaaga cgacattgtg
    29281 attaccgaaa ccggcaacga aaacctcacc gccagcgtgg tgaaaaagcc ggaagaaatc
    29341 gaagcgttga tggcagctgc gagaaagcaa tgagcgtaat catcgtcggt ggcggcatgg
    29401 cgggcgcgac gctggcgctg gctatttccc ggttaagtca cggggcgctg ccggtacatt
    29461 tgattgaagc gaccgcgcca gagtcacatg ctcatccggg ctttgatgga cgagcgatag
    29521 cgctggcggc gggtacctgt cagcaactgg cgcgcatcgg cgtctggcaa tctctggcgg
    29581 attacgcaac cgccatcact accgtgcatg tcagcgatcg tggtcacgct ggatttgtca
    29641 ccctcgccgc agaagattac caactggcgg tgctgggaca ggttgtcgaa ttgcacaatg
    29701 tcgggcaacg gctgtttgca ttgctgcgta aagcacctgg cgtaacgctg cattgccctg
    29761 atcgcgtggc taacgttgcc cgtacgcaga gtcacgttga agtgacgctg gagagtggcg
    29821 agacgctgac gggccgcgtg ctggtagcag ctgatggcac ccattcagcg ttagccaccg
    29881 cgtgcggcgt tgactggcag caggagcctt acgaacaact ggccgtgatt gccaacgttg
    29941 cgacttccgt tgcgcacgaa ggccgcgctt ttgaacgctt cacgcaacat ggcccgctgg
    30001 cgatgttgcc gatgtctgac ggacgctgtt cgctggtctg gtgtcatcca ctggaacggc
    30061 gcgaagaggt gctgtcgtgg agcgacgaga agttttgccg tgaactccag tcggcctttg
    30121 gctggcgact tggaaaaatt acccacgccg gtaaacgcag tgcttatccg ctggcgttaa
    30181 cccgcgccgc cagagctatt acccatcgta ccgtgctggt gggcaatgcg gcgcaaactc
    30241 tgcacccgat tgccgggcaa gggtttaacc tcggtatgcg agatgtcatg agccttgcgg
    30301 aaaccctgac tcaggcgcat gagcgcggag aagacatcgg tgattacggc atattgtgcc
    30361 gctatcagca gcgtcgacag agcgatcgcg aagcaaccat cggcgtcacg gacagcctgg
    30421 tacatctttt tgccaaccgt tgggcaccgc tggttgtcgg gcgcaacatc ggactgatga
    30481 cgatggaatt attcaccccg gcacgcgatg tgctggcgca gcgcaccctc ggttgggtgg
    30541 cgcgttgaag gcggttttaa ggaataaaca atgcaaagtg ttgatgtagc cattgttggt
    30601 ggcggcatgg tggggctggc ggttgcctgc ggcttacagg ggagcggctt acgcgttgcc
    30661 gtactggagc agcgcgtacc ggaacctctg gcggcggatg caccaccaca actgcgcgtt
    30721 tcggctatca atgccgccag cgaaaaatta ctcacccgtc ttggcgtctg gcaggacatt
    30781 ctctctcgta gggccagttg ttatcacggt atggaagtgt gggacaaaga cagttttggt
    30841 cacatttcgt ttgacgatca aagcatgggc tatagccatc ttgggcatat cgttgaaaac
    30901 tcagtgattc actacgcgct gtggaacaaa gcgcagcagt cgtcagatac cactctgttg
    30961 gcccccgcag aattacagca ggtcgcctgg ggagaaaacg aaaccttcct gacgctgaaa
    31021 gatggcagta tgttaacggc gcgtctggtg attggcgcgg acggcgctaa ttcctggttg
    31081 cgcaacaaag ccgatattcc gctgactttc tgggattatc agcatcacgc gctggtagcg
    31141 accattcgca cggaagaacc gcatgatgcg gtggcgcggc aggttttcca tggcgaaggc
    31201 attctggcct ttttaccgct tagcgatccg catctttgct cgattgtctg gtcactgtcg
    31261 ccagaggaag cgcagcgtat gcagcaggca ggggaagacg aatttaatcg cgcgttaaat
    31321 atcgcttttg ataatcgcct gggcttatgc aaggttgaga gcgcgcgtca ggtgttccca
    31381 ctgacggggc gttatgcgcg ccagtttgcc gcgcaccgtc tggcgctggt gggcgacgcc
    31441 gcgcatacca ttcacccgct ggcggggcag ggggtaaatc tcggctttat ggatgctgca
    31501 gagctggttg ctgaactgaa acggttgcat cgtcagggta aagacatcgg gcagtacatt
    31561 tatctgcgtc gctatgagcg tagccgcaag cacagtgcgg cgatgatgct ggctggtatg
    31621 cagggattcc gcgatctgtt ttccggtacc aatccggcga aaaaactgct gcgtgatatt
    31681 ggtctgaaac tggccgacac gcttcctggc gttaaaccac aacttatccg ccaggcaatg
    31741 ggattaaacg atttgcctga atggctgcgt taaaaatttc tcttctgctg tttatttgat
    31801 acccatcaca ctttcatctc ccggtttttt cgccgggaga ttttcctcat ttgaaataaa
    31861 ctaatttcac ctccgttttc gcattatatt ttctaatgtc acgaattttt gatttagtgt
    31921 tttttgacat tttttcagct cttaatattg tcttattcaa attaactt
//
LOCUS       DBANUU010000046        31930 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000046 DBANUU010000000
VERSION     DBANUU010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.046"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(256..1197)
                     /gene="fabY"
                     /locus_tag="ACIE68_002047"
                     /gene_synonym="yiiD"
     CDS             complement(256..1197)
                     /gene="fabY"
                     /locus_tag="ACIE68_002047"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFX5908360.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1242..1679)
                     /gene="dtd"
                     /locus_tag="ACIE68_002048"
     CDS             complement(1242..1679)
                     /gene="dtd"
                     /locus_tag="ACIE68_002048"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFX5908361.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1676..2548)
                     /gene="yihY"
                     /locus_tag="ACIE68_002049"
     CDS             complement(1676..2548)
                     /gene="yihY"
                     /locus_tag="ACIE68_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFX5908362.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2542..3141)
                     /gene="yihX"
                     /locus_tag="ACIE68_002050"
     CDS             complement(2542..3141)
                     /gene="yihX"
                     /locus_tag="ACIE68_002050"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFX5908363.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3240..4025)
                     /gene="csqR"
                     /locus_tag="ACIE68_002051"
     CDS             complement(3240..4025)
                     /gene="csqR"
                     /locus_tag="ACIE68_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFX5908364.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4059..4955)
                     /gene="yihV"
                     /locus_tag="ACIE68_002052"
     CDS             complement(4059..4955)
                     /gene="yihV"
                     /locus_tag="ACIE68_002052"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFX5908365.1"
                     /translation="MIRVACVGITVMDRIYYVKGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5123..6019
                     /gene="yihU"
                     /locus_tag="ACIE68_002053"
     CDS             5123..6019
                     /gene="yihU"
                     /locus_tag="ACIE68_002053"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFX5908366.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     SGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6043..6921
                     /gene="yihT"
                     /locus_tag="ACIE68_002054"
     CDS             6043..6921
                     /gene="yihT"
                     /locus_tag="ACIE68_002054"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFX5908367.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6938..8179
                     /gene="yihS"
                     /locus_tag="ACIE68_002055"
     CDS             6938..8179
                     /gene="yihS"
                     /locus_tag="ACIE68_002055"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFX5908368.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8317..9219
                     /gene="yihR"
                     /locus_tag="ACIE68_002056"
     CDS             8317..9219
                     /gene="yihR"
                     /locus_tag="ACIE68_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFX5908369.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9418..11454
                     /gene="yihQ"
                     /locus_tag="ACIE68_002057"
     CDS             9418..11454
                     /gene="yihQ"
                     /locus_tag="ACIE68_002057"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFX5908370.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAAALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            11500..12885
                     /gene="yihP"
                     /locus_tag="ACIE68_002058"
     CDS             11500..12885
                     /gene="yihP"
                     /locus_tag="ACIE68_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5908371.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            12928..14331
                     /gene="yihO"
                     /locus_tag="ACIE68_002059"
     CDS             12928..14331
                     /gene="yihO"
                     /locus_tag="ACIE68_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5908372.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFGNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            14399..15091
                     /gene="ompL"
                     /locus_tag="ACIE68_002060"
     CDS             14399..15091
                     /gene="ompL"
                     /locus_tag="ACIE68_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFX5908373.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(15182..16447)
                     /gene="yihN"
                     /locus_tag="ACIE68_002061"
     CDS             complement(15182..16447)
                     /gene="yihN"
                     /locus_tag="ACIE68_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5908374.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(16549..17529)
                     /gene="yihM"
                     /locus_tag="ACIE68_002062"
     CDS             complement(16549..17529)
                     /gene="yihM"
                     /locus_tag="ACIE68_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFX5908375.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(17537..18247)
                     /gene="yihL"
                     /locus_tag="ACIE68_002063"
     CDS             complement(17537..18247)
                     /gene="yihL"
                     /locus_tag="ACIE68_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFX5908376.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPKTLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(18464..20287)
                     /gene="typA"
                     /locus_tag="ACIE68_002064"
     CDS             complement(18464..20287)
                     /gene="typA"
                     /locus_tag="ACIE68_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFX5908377.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            20660..22069
                     /gene="glnA"
                     /locus_tag="ACIE68_002065"
     CDS             20660..22069
                     /gene="glnA"
                     /locus_tag="ACIE68_002065"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFX5908378.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            22388..23437
                     /gene="glnL"
                     /locus_tag="ACIE68_002066"
     CDS             22388..23437
                     /gene="glnL"
                     /locus_tag="ACIE68_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFX5908379.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            23449..24858
                     /gene="glnG"
                     /locus_tag="ACIE68_002067"
     CDS             23449..24858
                     /gene="glnG"
                     /locus_tag="ACIE68_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFX5908380.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            24970..25080
                     /gene="yshB"
                     /locus_tag="ACIE68_002068"
     CDS             24970..25080
                     /gene="yshB"
                     /locus_tag="ACIE68_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFX5908381.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(25270..26643)
                     /gene="hemN"
                     /locus_tag="ACIE68_002069"
     CDS             complement(25270..26643)
                     /gene="hemN"
                     /locus_tag="ACIE68_002069"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFX5908382.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(26832..27341)
                     /gene="yihI"
                     /locus_tag="ACIE68_002070"
     CDS             complement(26832..27341)
                     /gene="yihI"
                     /locus_tag="ACIE68_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFX5908383.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVIEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(27460..27626)
                     /locus_tag="ACIE68_002071"
                     /pseudo
     CDS             complement(27460..27626)
                     /locus_tag="ACIE68_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27923..28555
                     /gene="yihA"
                     /locus_tag="ACIE68_002072"
     CDS             27923..28555
                     /gene="yihA"
                     /locus_tag="ACIE68_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFX5908384.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(28937..31723)
                     /gene="polA"
                     /locus_tag="ACIE68_002073"
     CDS             complement(28937..31723)
                     /gene="polA"
                     /locus_tag="ACIE68_002073"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFX5908385.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPNDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT         7760 a         8254 c         7902 g         8014 t
ORIGIN      
        1 accagagggg gttttgacta tcaaaaatcg ctcaaacctg aactaagatg atgagttaag
       61 cattatctgc gataattgcc actcagctct acaaataata tttaaatgct cctgaacagt
      121 gatgtagcaa gacacgtatg gagccaacga agaataaaaa tttatagagt tcttattcaa
      181 tataaccgga atagtcaggg gctgatgtaa atgaccggaa tgagtaagag aggcagcacc
      241 gcccctctcc atcagctact cttcttcgtt cccgccctct tcatacgggc caaatggctt
      301 cgcgggcaga acgatatacg tgccttcaaa cactgcaccc ggcgtctcgt cgccaaagat
      361 ttcaacttgc atctgcaccc gtgcttttcg tccgcgcgcc agacggtcga gatcgccgct
      421 taaggcacca aggtcggcta ccgcatgagg tttaccgcta atcggcttgc tgtagcggat
      481 atgcgcatcc gccagaataa tcgttccgcc gagatggcgt tcgcgcagca tcagccagat
      541 aagcccccaa ccggtgagcg ttgccagtga gaataaactc ccggcaaaca gcgtatggtg
      601 cggattctga ttaccggttt ccggcatggt ggtgataaat ttttgcccgg tatattgttg
      661 aatacgcacg cccatttttt cactaagcgg gatatgttcg taccacgcct gttgcagctg
      721 cgcgcaccag tcgccgcgat gcaaaatatc atccagagtg gcaacgggtt taatcatcaa
      781 aaaatggcga atcggcgtgg tagttggggt ggtgatctcc ccctgattaa tgaatcccag
      841 cttggcgaaa aactccaccg cgtcttcacg ggcgctacag gtcacgcgct ttacgccttc
      901 ctgacgcgcc accgactcca gggtcatcgc catcagcgtg cctaaccctt tgtcctgcac
      961 gtccgggtga acggccataa agcgaatgga cgcttcattg tcggcattaa tatacaatcg
     1021 gcctaccgcc accagattac cctgctcgtc gacgaccatc tggtgatgcg ccatcgcatc
     1081 ccacgcgtcg cgttccgaac cttttggttg atgcaggggc ttacgcaaca tttcccagcg
     1141 aaactgatag taacgctcta attcttcttc tgtttgtgga acccgaaggt gatacatagc
     1201 gatactctct cttgtttccc gtgacaaccc tggaagctgg ctcatacctg caaccagaat
     1261 gtcacggggc catcattgac cagcgatacc tgcatatccg cagcgaagcg tcctgtttgc
     1321 gtgttcatct cttgctgacg gcagcgttcg acgaaatagt catataacgc ctctgcgcga
     1381 tccggtgatg cacctttgga gaaacttggg cgcatccccc gttcggtatc tgcggcgagg
     1441 gtaaactggg aaaccaccag cacactgccg cccgcctgtt gcacgttgag gttcatcttg
     1501 ccttcggcat cgctaaagat gcggtagccg agcacacgct cgcacagacg gtttgctttc
     1561 tgttcgtcgt catccttttc gacacccaat aacaccaaaa gtcccgcgcc aatttcgccc
     1621 gtcacttctc cctccacggt gacgctggca cgggttacgc gttgaattaa tgcaatcatg
     1681 gttcgtcgtc ttcttcttgt tcagctgctt gttttagttt gcggtattcc ccgagagtga
     1741 cagtaatttc cgcgccaagc aagacgatac accatgtcca gtagacccaa acaaagagaa
     1801 tggggatcac cgccaacacg ccgtaaatga gctgatatga cggaaacatg gtgatataaa
     1861 gcgcgaaacc tttctttcct gcttcgaaca ggagcgcggc gacaaacgcg ccgacaatcg
     1921 cgtcgcggtt aggtacgcgg atggtaggaa caatgctgta cagcaaccag aaggagatcc
     1981 acgacaacag cagcggaaaa atacgcagca cgttatcgat gacagtattg agatcgctcg
     2041 cccagcgcag ggagagcaaa taggaactga tcgccagact ggcccctgcc agcagcggcc
     2101 ccagcgttaa aatcatccag tacacggcga acgagtaaat ttttggtcgc gctcgtttac
     2161 tgcgccagat ggtattcaac gcgctatcga tggagtacat cagcaataac gcggtgacga
     2221 tcagcccgca cgccccaacg gcggtcatct tgttggaatt ggcaacaaat tgttcgatat
     2281 accgctgaat aacatcgcca gtagcaggca gaaagttggc aaaaataaag tgacgtaact
     2341 gaatgctgac gtcggaaaac atgggaaaag cggcgaaaag cgcaaaaaca acggcaacca
     2401 gcggcactaa tgagagcaac gacacatagg caaggttacc tgccagggtt gtcatgttgt
     2461 cctcatcaat gcgttgccag agtagtttta gccaggccca tagtggacgg gtacggtgcc
     2521 tggctttgtc ctgaatggtt tttagcataa caccttcgcg aaatagtccg ggatggtggt
     2581 tttatctttc accagaatac tggtaatgcc cagctgattg gctccttcta tattatcggc
     2641 gttatcgtcg aaaaagaccg tatcgctggg tgaaaaacct tccgcctgca aaacatgctg
     2701 gtaaattcgt gcttcaggtt tgcgcatccc cagatcttgc gacagataga tatggtcagc
     2761 agcatcacga atttccgggt attcttccgg ccagaaggtg gtatgcaggc ggttggtatt
     2821 ggaaagcacc accacgcgat gcccctgctc acgcagttta tgcatgatgg cgatcacttc
     2881 cgggcgcagc gcaacaaaca ccgcctgcca gccgtgagag aactgctcgt agcttagcgg
     2941 tagagccatc tcatgacaca gcgcctctgc gaacgcttcg tcgctaattt ccccacgctc
     3001 atgctgatga aacgcctccc ccatatgaaa actcttctta agcgatgcca gcggaatacg
     3061 cgttaaatcg ctccaggctc ccagcacacg gttaaagtcg atatcgacaa tcacattacc
     3121 taaatcaaag atatagagca tttttgcctc ctttcgcgcc acaagaaatt aactgtagcg
     3181 ggaaaggatg ggtttggcta tgtggcaagg tatgaagcca ggttaacggt cacaaaaatc
     3241 tacgcgtctt cctggggaag aatgatttca atttggctgt cttgtaagcg tgattttagc
     3301 gcagcgggcg gcagctcgtc ggtaaagagc gcagtgacct gtgccacgtt accaatttca
     3361 accgccgccg aagcatgata cttagtgtga tcggcgacca gcagaatatt tctcgcgtgc
     3421 gccatcatcg ttttcaccac gttagcttcg tttacatcaa actccatcaa cgcgccatcg
     3481 ctctcaatcg ccccaacgct tgttaccaga taatcagcac gaaaatcagc cacaaaggac
     3541 gccgctgaag ggccaatgat cccgctatta tgagagcgca acgtaccgcc gggcaccatc
     3601 acttcaaagc gcgggttgtg gtaaagaata tgcgccacac gcaggctgtt ggtgattatc
     3661 cgcaaatgat tatggttaag taacgcccgg gcaacatgct caacagtcgt accaatggtg
     3721 ataaatattg ttgaaccatc aggaatatag tctgccaccg cttcggcaat cgcttttttt
     3781 tcctcggttt gcgaaacctc acgctgctcg aacgccgtat taacgacgct ggaagcccga
     3841 cccgcgccac cgtgatggcg cgtaatcagg ccttgctcgc ttaatttacg aatatcccgg
     3901 cggaccgtct gcgtggagac atccagcaga tttgccagct catcaatatt catataccca
     3961 cgctcggcga tcagcatgag gagttggtcg tgccgcgggt taccggttag ttcggtaagg
     4021 ctcatgaaaa ttcctcgaaa aaccatcacc ctggcatttt atacaaaaag tgacaaaaaa
     4081 gatcgggttt gatcacagtc agggatcccg gctcgtccac cgggacgtgt gcattttaac
     4141 gccgctacac cgctggcgaa gcggactgac tccgctaaat ccccacttgt tgccagcgcc
     4201 accgccaaag cgccgtgaaa aacatcaccc gcacctgtgg tatctaccac atcaactttg
     4261 aaggccggtt gatgctgacg cccaccattt tccagccagt cgcagcctgc gctaccctgg
     4321 gtcacataga catgaccatt tgtgagcgtt tgtgcctgtt ttagcgcact ggccatctct
     4381 ttcacgcccg ttaagcgcgc cagacccggt tctgaaaagg ccgcgtgatc gcttaatgcc
     4441 accagctcac tgatatcctg cggcgtaatg tccccgtcca gaacggtcat cacacccgcc
     4501 tgacgggcca gggtgaaggc ttttttagcg ccgtcgtgcc agcgtacatc tgccagcaca
     4561 acatcccact gagagaaatc aatttcctcc aaccactctg cgtcaggcag cagatccggg
     4621 ctggggtagt taatgattat ccgctcgcct ttggtatcca ccatgatggc ggattgcgaa
     4681 gatttcgcct ggttataccg tttggtgtaa cgggtgttaa cgccccagga ttccagctct
     4741 gccagcaggc tgttgcccgt gtcgtcatca cctacgcgac caataaaatc gacctgcgcc
     4801 cccagccttg ccgccgcaac cgctgccgtc gccgctggcc cgccaccaac ttccgtataa
     4861 tttctcgcca cgtatttacc gctctccgtc ggtaaccctt tcacgtaata gatgcgatcc
     4921 atcacggtta tacctacaca agcaacacga atcatggtca ttccttaaac attttgggat
     4981 gcgttcattt taatttcacc aattgtttaa aaagtgacgg ctgtcaattt tttgacgata
     5041 aattacaaat acgatcaaaa acagacaagc aaagcatgtc ataaatgaca aaaagtgaca
     5101 tcactgtatt caggagaggg ttatggcagc aatcgcgttt atcggtttag gacaaatggg
     5161 ttcgccaatg gcgagcaatt tattgcagca agggcatcaa cttcgcgtct ttgatgtgaa
     5221 cgccgaagct gtgcggcatc tggtagacaa aggtgcgact cccgccgcca acccggcgca
     5281 ggccgctaaa gatgccgaat ttatcattac catgttgccg aatggcgatc tggtgcgcaa
     5341 cgtgttgttc ggtgaaaacg gcgtttgcga aggcttatct accgatgcgc tggtcattga
     5401 tatgtccacc atccatccgc tgcaaaccga taaattgatt gccgatatgc aagccaaagg
     5461 cttcagcatg atggatgttc cggtaggccg tacttctgca aatgccatta ccggtactct
     5521 gttactgctg gctggcggca ccgctgaaca agttgaacgt gccacaccga tcctgatggc
     5581 gatgggcagt gagttgatca actctggcgg tccgggcatg gggatccgcg ttaagctcat
     5641 caacaactac atgagcatcg cgctcaatgc gctttcggca gaagccgccg ttttgtgcga
     5701 agccctgaat cttcccttcg atgttgccgt caaagtgatg agcggtaccg ccgccggtaa
     5761 aggtcacttc accacttcct ggccaaacaa agtcctcagc ggagatcttt ctcccgcctt
     5821 catgatcgat cttgcccata aggatcttgg catcgccctt gatgtcgcca accagctgca
     5881 tgtgccaatg ccgctggggg ccgcctcacg ggaggtttat agccaggcgc gcgcagcggg
     5941 tcgcggtcgc caggactggt ccgccattct ggaacaggtc cgtgtcagtg ccgggatgac
     6001 tgccaaagta aaaatgtaac gactggataa aggaataaat gaatgaataa gtacaccatc
     6061 aacgacatta cgcgcgcatc gggcggtttt gccatgctgg cggtcgatca gcgcgaagcc
     6121 atgcgcatga tgtttgccgc ggctggggca cccgctccgg tagccgatag cgttttaact
     6181 gatttcaaag ttaacgctgc aaaggccctc tcgccttatg cctcggcgat tctggtagat
     6241 caacaattct gctaccgcca ggtggttgag caaaacgcga ttgccaaaag ttgcgccatg
     6301 attgtcgccg ccgatgagtt cattcctggc aacggtattc cggtcgatag cgtggttatt
     6361 gaccgcaaaa tcaatccgct acagatcaaa caagacggtg gcaaagcctt aaaactgctg
     6421 gtgctgtggc gtagtgatga agatgcgcag caacgtctgg atatggtgaa agagttcaac
     6481 gaactgtgcc actcacacgg tctggtaagc atcattgagc cagtcgtccg cccaccgcgt
     6541 cgtggcgata aattcgatcg cgaacaagcg atcatcgatg ccgccaaaga gctgggcgac
     6601 agtggcgctg acctctacaa agttgaaatg ccgctttatg gcaaaggtcc gcaacaagag
     6661 cttctctgtg cttcacaacg tctgaatgac catatcaata tgccatgggt gatcctctct
     6721 tccggtgtcg acgaaaaact gttcccgcgt gccgtacgcg tggcaatgac ggcgggcgca
     6781 tcgggattcc tggcaggccg tgcagtctgg gcatcggtcg tcggtttacc agacaacgag
     6841 ctgatgctgc gtgacgtttg cgcaccgaaa ttacaacaac ttggcgatat cgtcgacgaa
     6901 atgatggcta aacgccgcta agatgaggat aaaaggaatg aaatggttta acaccctaag
     6961 ccacaaccgt tggctggaac aggaaaccga ccgcatcttt gattttggta aaaattccgt
     7021 agtgccgact ggttttggct ggttaggcaa taaagggcaa atcaaagaag agatgggcac
     7081 ccatctgtgg atcaccgctc gtatgttgca cgtttattcc gttgctgcgg cgatgggtcg
     7141 acctggcgct tactcgttgg ttgatcacgg tatcaaagcc atgaacggcg cactgcgtga
     7201 taaaaaatat ggcggctggt atgcctgcgt gaatgacgag ggcgtggtgg atgcctccaa
     7261 acagggctat caacacttct ttgctctgct gggtgccgcc agcgccgtca caacgggtca
     7321 cccggaagcg cgcaagctgc tcgattacac cattgaaatt atcgagaaat atttctggag
     7381 cgaagaagag cagatgtgcc tggaatcctg ggacgaagcc ttcagcaaaa ccgaagagta
     7441 ccgcggcggc aatgccaata tgcacgcggt ggaagctttc ttgattgttt atgacgtcac
     7501 tcacgacaaa aaatggctgg atcgcgcgat tcgcgtggct tccgtgatta tccacgacgt
     7561 cgccagaaat aatcattatc gcgttaacga acacttcgat acccagtgga atccgctgcc
     7621 ggattacaac aaagataacc cggcgcatcg cttccgcgcg tttggaggta caccaggcca
     7681 ctggatcgaa tggggccgtt taatgctgca catccatgct gccctggaag cccgttgcga
     7741 acaaccacca gcatggctgc tagaagatgc caaaggtctg tttaacgcca ccgtgcgcga
     7801 tgcctgggca cccgatggcg cggacgggat tgtttatacc gttgactggg aaggaaaacc
     7861 ggtggtccgc gaacgtgtac gttggcctat cgtcgaagca atgggtacgg cctacgcgct
     7921 ctacaccgtc actggcgatc gccagtatga aacctggtat caaacatggt gggagtactg
     7981 cattaagtac ctgatggact atgaaaatgg ttcctggtgg caggagctgg atgcggacaa
     8041 taaggtcacc accaaagtct gggacggcaa acaggatatt tatcacctgc tgcattgcct
     8101 ggtgatcccg cgtatcccgt tagcgcccgg catggctcca gcggttgcag cgggtctgct
     8161 ggatattaat gcgaaataac tgatctgaaa atagggtgcg ggttttcccc tcaccctaac
     8221 cctctcccca aaggggcgag gggaccgtcc ggtgcagttt ttgggtttga catcagcctc
     8281 acgggaggct gtatgtcgtt aattaaggtg ccttgtatgc aaatcaccaa tatgcattgc
     8341 agtggacaga ccgtttcact tgccgcgggg gattatcacg cgaccattgt gaccgtcggt
     8401 gctggtctgg ctgaattaac ctttcagggt tgtcatttag tcatcccgca caaacccgaa
     8461 gagatgccgc tggcgcatct gggtaaagtg cttattccgt ggccgaaccg gatcgccaac
     8521 ggctgttacc gctatcaggg gcaggaatat cagttaccca ttaatgaaca tagttcaaaa
     8581 gccgctattc acgggctgct tgcctggcga gactggcaaa ttagtgaatt aaccgccacc
     8641 agcgttaccc tgacagcttt tctgccgccc agttacggct atccgtttat gctggcttca
     8701 caagttgttt attcgctcaa cgcacacacg ggtctttccg ttgagatcgc aagccagaat
     8761 atcggcactg tcgcagcgcc ttatggcgtg ggtattcata cttatttaac ctgcaactta
     8821 acatctgttg atgaatatct gttccagctt ccggcaaacc aggtttatgc cgttgatgaa
     8881 cacgtaaatc cgacaacgct tcaccacgtt gatgagctgg atttaaattt cacgcaggcg
     8941 aaaacaatag ccgcgacaaa aatcgaccac acctttaaaa ccgctaatga tctgtgggaa
     9001 ataaccatta cccatccaca acaggcgctt tcggttagcc tttgttgcga tcaaccctgg
     9061 gtacaaattt atagcggtga aaaattacag cgtcagggac tggcagttga acctatgagt
     9121 tgcccgccta acgcctttaa ttcaggtatc gacttattat tactggaacc aggaaaaccg
     9181 caccgcctgt tttttaacat ttacggacag agaaaataac ttgtattgat tagcagtcaa
     9241 aatcattgaa gaacatttca ctcgctaaag gcaacattat gtatcttcaa tttattaagt
     9301 taaaaacagc tcagcttctg taaagttttt atatatcagt tttatgagaa tcattttaca
     9361 taagcctgta ataacaggaa ggcaacaatc tcgttctcaa ctctatggag attagttatg
     9421 gatacgccac gtccacagtt attagatttt caatttcatc agaataacga cagttttacc
     9481 ctacattttc aacaacgtct tattttaacc catagcaaag ataatccttg tttatggatt
     9541 ggctcaggta tagcggatat cgatatgttc cgcggtaatt tcagcattaa agataaacta
     9601 caggagaaaa ttgcgcttac cgacgccatc gtcagccagt caccggatgg ttggttaatt
     9661 catttcagcc gtggttctga cattagcgcc acgctgaata tctctgccga cgatcagggc
     9721 cgtttattgc tggaactaca aaacgacaac cttaaccaca accgtatctg gctgcgcctt
     9781 gccgctcaac cagaggacca tatctacggc tgcggcgaac agttttccta cttcgatctg
     9841 cgtggcaaac cgttcccgct atggaccagt gaacaaggcg ttggtcgcaa caaacaaacc
     9901 tatgtcacct ggcaggccga ctgcaaagaa aatgcgggcg gcgactatta ctggactttc
     9961 ttcccacagc ctacgtttgt cagcacgcag aagtattact gccatgttga taacagttgc
    10021 tatatgaact tcgactttag tgccccggaa taccatgaac tggcgctgtg ggaagacaaa
    10081 gcaacgctgc gttttgaatg tgctgacaca tacatttccc tgctggaaaa attaaccgcc
    10141 ctgctgggac gccagccaga actgcccgac tggatttatg acggagtaac gctcggcatt
    10201 cagggcggga cggaagtgtg ccagaagaaa ctggacacca tgcgtaacgc gggcgtgaag
    10261 gtcaacggca tctgggcgca ggactggtcc ggtattcgta tgacctcttt tggcaaacgc
    10321 gtgatgtgga actggaagtg gaacagcgaa aactacccgc aactggattc acgcattaag
    10381 cagtggaatc aggagggcgt gcagttcctg gcctatatca acccgtatgt tgccagcgat
    10441 aaagatctct gcgaagaagc ggcacaacac ggctatctgg caaaagatgc ctctggcggt
    10501 gactatctgg tggagtttgg cgagttttac ggcggcgttg tcgatctcac taatccagaa
    10561 gcctacgcct ggttcaagga agtgatcaaa aagaacatga ttgaactcgg ctgcggcggc
    10621 tggatggctg acttcggcga gtatctgccc accgacactt acctgcataa cggcatcagc
    10681 gccgaaatta tgcataacgc ctggcccgcg ctgtgggcga aatgtaacta cgaagccctt
    10741 gaagaaacgg gcaagctcgg cgagatcctt ttctttatgc gcgccggttc taccggtagc
    10801 cagaaatact ccaccatgat gtgggcgggc gaccagaacg tcgactggag tctcgacgat
    10861 ggcctggcgt cggttgtccc ggcggcgctg tcgctggcaa tgaccggaca tggcctgcac
    10921 cacagcgaca ttggcggtta caccaccctg tttgagatga agcgcagcaa agagctgctg
    10981 ctgcgctggt gcgatttcag cgccttcacg ccgatgatgc gcacccacga aggtaaccgt
    11041 cctggcgaca actggcagtt tgacggcgac gcagaaacta tcgcccattt tgcccgtatg
    11101 acctccgtct tcaccaccct gaaaccttac ctgaaagagg ctgccgcgct gaatgcgaag
    11161 tccggcctgc cggttatgcg cccgctgttc ctgcattacg aagacgatgc gcacacttac
    11221 accctgaaat atcagtacct gttaggtcgc gacattctgg tcgctccggt gcatgaagaa
    11281 ggccgtagcg actggacgct ctatctgccg gaggataact gggtccacgc ctggacgggt
    11341 gaagcgttcc ggggcgggga agttaccgtt aatgcgccca tcggcaagcc gccggtcttt
    11401 tatcgcgccg atagcgaatg ggcggcactg ttcgcgtcgt taaaaagcat ctaatcacat
    11461 ccgcccgtgg ggaaactcat gggcaaaggg agaacatcga tgagtcacat cacaacggaa
    11521 gatccagcaa ctttacgcct gccctttaaa gagaaactct cttacggtat tggcgacctg
    11581 gcctctaaca tcctgctgga tatcggtaca ctttatcttt tgaagtttta taccgacgtt
    11641 ctggggctgc caggcaccta tggcggcatt atctttttga tttcaaaatt ctttactgcg
    11701 tttaccgata tgggtaccgg cattatgttg gattcccgac gcaagatcgg tccaaaaggt
    11761 aagttccgtc cttttattct gtatgcgtca ttcccggtca ccttactggc gatcgccaac
    11821 tttgtcggca caccgtttga tgtcaccggt aaaacggtga tggccactat tctgtttatg
    11881 ctttacggac tgtttttcag catgatgaac tgctcctacg gcgcaatggt tcccgctatc
    11941 accaaaaacc ccaacgaacg cgcctcactg gcggcatggc gtcagggcgg cgctacgctg
    12001 ggcctgctgc tgtgcacggt gggattcgtg ccagtgatga atcttatcga gggtaatcag
    12061 caacttggct atatcttcgc cgccacgctg ttttcactgt tcggcctgct gtttatgtgg
    12121 atctgctact caggcgtgaa agagcgttat gtcgaaaccc aaccaaccaa tccggcgcaa
    12181 aagcctggcc tgttgcaatc tttccgcgca attgccggta accgcccact gttcattctg
    12241 tgtattgcca acctctgcac cttaggggcg tttaacgtca agctcgccat tcaggtctat
    12301 tacacccagt acgtacttaa cgatcccatc ctgttgtcgt atatgggatt tttcagcatg
    12361 ggctgtattt tcatcggcgt gttcctgatg cctggcgcag tcagacgttt tggtaagaag
    12421 aaggtctata tcggcggcct gctgatttgg gtgctgggcg atctgctcaa ctatttcttc
    12481 ggcggcggct cggtcagctt tgtggcgttc tcctgcctgg cgttcttcgg ctcagcgttt
    12541 gttaacagcc tgaactgggc gctggtttcc gacaccgtcg agtacggcga gtggcgcacc
    12601 ggcgtgcgtt cggaaggcac ggtctacacc ggcttcacct tctttcgcaa agtgtctcag
    12661 gcgctggctg gtttcttccc cggctggatg ctgacgcaaa ttggctatgt gccgaacgtc
    12721 gcacaggctg accacaccat tgaagggtta cgccagttga tcttcatcta cccaagcgca
    12781 ctggcggtag tcaccattgt ggcgatgggt tgcttctaca gcctgaacga gaagatgtat
    12841 gtccgcattg tggaagagat agaagcacgt aaacgcacgg cgtaataata ataaacgacg
    12901 ccctgcgggg cgttataagg agtgattatg tctgaccata atccactgac attaaaactg
    12961 aatctgcggg aaaaaatcgc ctatggtatg ggcgacgtcg gttcgaattt aatgctctgc
    13021 atcggtactc tgtatctcct caaattttat accgatgaat tagggatgcc tgcttactat
    13081 ggcgggatta tctttctggt ggcgaagttt tttaccgcgt ttaccgatat gctcaccgga
    13141 tttttactcg actcgcggaa aaatattggg cctaaaggca aatttcgtcc ctttatttta
    13201 tatgcagcag ttccggcggc gttaattgca acgctccagt ttatcgccac caccttttgt
    13261 ttgccggtta aaacgacgat tgccaccgcg ctgtttatga tgtttggact ttcatacagt
    13321 ctgatgaact gctcgtatgg tgcgatgatc ccggcaatta ccaaaaaccc gaatgagcgc
    13381 gcgcagctcg cggcttaccg ccagggtggt gcaaccatag ggctattgat ttgtaccgtg
    13441 gcgtttattc cgttgcagtc gcttttttct gactcaaccg tcggttatgc ctgtgcggca
    13501 cttatgttct ccattggcgg ctttattttt atgatgctgt gctacagagg cgtcaaagag
    13561 cattatgtgg acacagcgcc aaccggacat aaagccagta ttctcaaatc tttttgcgcg
    13621 atatttggta atccaccatt gctggtttta tgcattgcta acctgtgtac tctggcggca
    13681 tttaatatca aactggcgat tcaggtctat tacacccagt atgtgctgaa tgatattaat
    13741 ttattgtcgt ggatgggatt cttcagtatg ggatgcatcc tcgtcggcgt attactggtg
    13801 ccagtaactg taaaatgttt tggtaaaaaa caggtttatt tagctggcat ggtgctgtgg
    13861 gcggtgggtg atatactgaa ttatttctgg ggaagtaact ctttcacttt cgtcatgttc
    13921 tcttgtgtcg ccttttttgg cacggccttt gtaaacagcc tgaactgggc actggtacca
    13981 gataccgtag attacggtga atggaaaacc ggtattcgtg ccgaaggttc tgtttatacc
    14041 ggttatacct tctttcgtaa aatttctgcc gcacttgctg gcttcttgcc aggcattatg
    14101 ctgacgcaaa ttggttatgt tcccaacatc gcgcaaagcg atgcgacatt acagggtttg
    14161 cgtcagctca tttttatctg gccttgtgca ctggcaatta ttgctgcatt gacgatggga
    14221 ttcttttaca cactcaatga aaaacggttt gcattgatta ttgaggaaat caaccaacgc
    14281 aaaaataaag agatggcgac agaagaaaaa acggcttccg taacgttata aatgtcaata
    14341 gccgctattt ccatcctggt gggtggcggc ctccctacgt ttaaaaaatg gacttattat
    14401 gaaaaagatt aatgcaataa ttttattatc ttctctgacc tctgcctcgg tatttgctgg
    14461 cgcatacgtc gaaaacaggg aggcatataa tcttgcctcc gatcagggcg aggttatgct
    14521 acgtgtgggt tataacttcg atatgggcgc gggtattatg ttaaccaata cctacacctt
    14581 tcagcgagaa gatgaactaa aacatggcta taatgaaatt gaaggttggt atcctttatt
    14641 caaaccaacc gataaattaa ccatccagcc cggtggctta attaatgata agagtatcgg
    14701 ttcgggtggt gcagtgtatc tggacgtcaa ctataaattt gtaccatggt ttaatctgac
    14761 agtacgaaat cgctataacc ataataacta tagttcaaca gatttgagcg gggaactgga
    14821 taataatgac acctatgaaa ttggcaccta ctggaatttt aaaatcaccg ataaattttc
    14881 ctatacattt gagccacatt atttcatacg cgtaaatgac tttaatagca gcaatggaaa
    14941 agatcatcac tgggaaatca ctaatacctt ccgttatcgc attaatgaac actggctgcc
    15001 ttatttcgaa ttacgttggt tagatcgtaa tgtcgaaccg taccaccgcg agcaaaacca
    15061 gattcgtatc gggacgaagt atttcttctg atgtgaaact gccggatgcg gcgtaaacgc
    15121 cttatccggc ctacggttga tacggttttc gttggccgga ttaagcacat ccggccatta
    15181 atcaagcttc agccgccagc atctctttct tacgttggct aattcgctta accagcatta
    15241 cggaaaccac cgcaccacag aatgcgaagc aggccataat gccaaacacg attttgtagc
    15301 caataatccc cggatttaaa tccagaatgt agccatacag actgaagcag aacatcgccg
    15361 gagcgtaacc aataaagcta cccaacgcca tcgccgcgcc tgttttattt tcagcaattt
    15421 ttgcttcgcc gataggtgca aaaaatacgg ctcgctgtgt aaagactatc gcgccaaagc
    15481 ccagcgtaca tgccatccct aaatagaccg gcatactttc gtgcggcagc ataatcaaca
    15541 gtacgagcgc agcggtactg atgataaagg tgtagcatag atatttactc ggcgatttca
    15601 ggatcttatc tgaaatcatg ccaccaatcg gtccaccaat cattttcaga cagtattggt
    15661 taatgatgcc gtaagccccc accagcgcaa cgggcaatag atagatgttt ttcaggaatg
    15721 gaatgaagaa tgtcaggcca cagtaaaccg cgtaaacaaa gaagacgtta aaagcgataa
    15781 gccagatggt tttgtctctc agcaccgagg tcatactggt gttttgcgat gctccgtctt
    15841 cttttttcac ctcaacggac ggtgcctctt ctttgtcatt caggacaaag aaaataataa
    15901 tgcctacggc aatcacaata agggaataga accagatgcc tgctttaaaa cctaataagc
    15961 cactgccaaa ccaggtaaat actgccaacg cagaaaatgc caccacggta tcgacaatgc
    16021 cacgccctgt ttcgaagaag ccaaacaacc gaccttgttg ttcgctattg cccaatcgac
    16081 ttaccgactt gagcaagacc ggccagttca tcatgtcgca agtaacgcca aatagcgccc
    16141 agacaaagag gatgccccaa taccccggca ttgtcgtcaa ataaacaccc agtaatcctg
    16201 tcgcaatgag tgagaatgac atggtgtatc tgcgcggtag tttatcggca aaataaatag
    16261 acagaaaaaa gcccactgtg gtgacaaatg agtttaccga catagcatta ccaatttgac
    16321 cattggtcaa atggaaatat tcctgcatcg ggatataaaa cgcatctttc agcgacggta
    16381 atttataaat tgtaccgcca cacagtgtta atagactaaa taacgcccat ttctttttcg
    16441 tgagcataac atcccctaag cgatttttaa aatcactgtt ttaaaggcaa taagataccg
    16501 accattcata tagaatggtt ttattaactg cgtatgagtt aaaacttatt agtggttcag
    16561 aagatggttt gcctcttgtt tgatttgttg tagtacgtta cgcacatgat ttatctgatt
    16621 aattgcatct tctttttctt tacgtaaccg cgcatccagt aaatgatttt ctggtagggg
    16681 tgtttcactc atctggcgat aagggatcca cggattatca tcttcgtctt caaagcggaa
    16741 cgccggcgca tagtaatcaa cctcttcttc caggccatat gccgtcacct gcggctcatc
    16801 atcaccaagg cagataaggt gcgttaataa cgctttgaac ggcatatcac cctgacctga
    16861 aatacaggct ttatgaccca ggccaccctg ttctttaacg atcaaggcat ctttaatatg
    16921 gacctgggta atatgcggtg ccatcgtttt taaggcgtcg atgggatgct catttgcatt
    16981 gatcatgttc gcaaaatcaa acagtaagga aagagactcc atctcacttt ctttgaccag
    17041 cgacaccaac tcatgacttt ttaaatcttc atgctgctcg atcgtaaaag tcaggccgct
    17101 gtcctgatac gtttcccgta cataggcaat gtcgttagcg ataatcgata atacgtcgcg
    17161 caaattacct tcataacgtg gataaaaacg tacggacgat gccccagttt tcaacgcgat
    17221 ggcgacggct tcgtcaatag atgccttatc tgaggcgctg gtttcaatat gaatatcaag
    17281 gttcagacgg cgggctttat ccccaaaggc agagagttct ttatcgtcca tattaccaag
    17341 agaaaaacgc tcgccatcca gaacatggat tttgacgcca cgtagattat tttcacttgc
    17401 gatatcaagt aaatcagcag gcaacactct ttccagccgc atatttaaat gaaaggcata
    17461 agcatgtaaa taaagaggaa gagtgtcgac acgttgtaga atctttcttg cgttatttat
    17521 tgtcaccata aaacacctac agattaatga tgaggtctac ggcctcaaat cgccaatatt
    17581 caatatctat ctggacaata ttgccttgct ggtcggcgcg atgcttttca attaagatcg
    17641 ctggcatacc cgcagaaccg ccaagatact tgctgatatc gcccggcatt ctgacgggtt
    17701 tgaaaatcaa tttttttact accgtctctt tttggtagca cttttcccag actgcagaaa
    17761 atgagtggtt ttcaagttgt tcaataaaac ccggagcaac ttcaggatta atatatgttt
    17821 cgtgatagaa aactttatgt ccttccagcg ccccccagcc ggtgatgcga tagagttcag
    17881 ttgatggtgt cactgcaatc agtggcgcga gcgtcttggg aatatcgctg gtacggtttt
    17941 tctcggtaaa cccccaggaa ggctctcgtc cttgctcaat tgcggcccgc tgaaagctcg
    18001 ccgacaactc cggactgtaa ttaaatcgcg gctgggtcac gaaccacccc ttacgatcct
    18061 tacggaagat tttggattct gcctcgaggt tcaacaacgc ctggcgcagc gtcatacgtt
    18121 taatgcccag cagttctccc agctctcttt ctgaagggag ttttccaccc ggcgtggtga
    18181 tgccatcttt caaccaccga tccagtttct cttttgcatt ttctacggtg gattgattct
    18241 ccgccattgt ttaatccttt tggtttaaac cagatgaagc atcttacgct tacgccaaaa
    18301 aagaaagcaa ttaacggcgt atgactttgc agataattgt gacaaagctc tctattgacg
    18361 taatcccaga cttataaggg agatactcgc attggtgtgc gctcacgaca ggtaaaaaaa
    18421 aacctgccag cgatggcagg ttttttataa ctaaaaaacg aaattaatcg tctttcggtg
    18481 cgcggttggc gcgacgacga tcgttttcag tcaggtgacg tttacgaata cggatagagg
    18541 tcggagtcac ttctaccagt tcgtcatcat cgatgaactc cagagcttgt tccagagtca
    18601 tgcggatagg cggaaccaga acaacggctt cgtcagtacc ggaagcacgc atgttggtca
    18661 gtttcttacc agtcaggcag tttaccgtca ggtcgttaga gcggctgtga ataccgataa
    18721 tctgaccttc gtaaacttct gcaccgtgac cgaggaacag cttaccgcga tcctgcagac
    18781 cgaacagcgc gaacgcgacc gctttaccct gaccgttaga gatcagtacg ccgttctgac
    18841 gctgacccac ttcacccgga cgtacgtcgt cgtagtggct gaaggtggag tacagcagac
    18901 cagtaccgga agtcatggtc atgaactcag aacggaagcc aatcagacca cggcttggga
    18961 tcacgtagtc gagacgtacg cggcctttac cgtctggatt catgtttttc aggtcgcctt
    19021 tacgttcgcc cagcgcctgc attacagaac cctgatgctg ttcttcaacg tccagagtca
    19081 cgttttcata cggctcttgt ttacgaccgt cgatttcacg gaagataact ttcggacggg
    19141 ataccgccag ttcgaaacct tcacgacgca tgttttcgat cagaacagac aggtgcagtt
    19201 cgccacgacc agaaacgcgg aacgcatcgg cgtcttcggt ttcttctacg cgcagcgcaa
    19261 cgttgtgtac cagttctttg ttcagacgat ccaggatctg acgagacgtt acgaacttac
    19321 cttctttacc gcagaacggc gaggtgttaa cgcagaagaa catagaaacg gtcggctcat
    19381 caacggagag tgccggcagc gcttcaacgt tttgcgtgtc gcaaacggtg tcagaaatgt
    19441 tcagttcgcc aaggcccgtg atcgcaacga tatcgccagc ttccgccaga tcggtttcga
    19501 tacgttccag accgaggtgg cccagcactt taccgacttt cgcgttgcgg gttttgcctt
    19561 cgctatcgat gatagtgacc tgctggttcg gcttcacttt accgcgcttg atgcggccaa
    19621 tgccgataac gccaacatag ctgttgtaat cgagctgaga aatctgcatc tggaacggac
    19681 cgtcaaggtc aacgtccggc gcaggaacgt gatcaacaat cgcctgatac agcggggtca
    19741 tgtcttccgc catatcttcg tggtccagac ccgcgatacc gttcagcgca gaagcgtaaa
    19801 cgatcgggaa gtccagctgc tcgtcggtcg cgtcgaggtt aacgaacaga tcgaatacct
    19861 gatccacaac ccaatcagga cgcgcgccag ggcggtcaac tttgttgata acaacaatcg
    19921 gcttcaggcc gtaagcaaac gcttttttgg ttacgaagcg cgtttgcggc atcgggccgt
    19981 caaatgcgtc aaccaccagc agcactgagt ctaccatgga cattacacgt tcaacttcac
    20041 caccgaagtc ggcgtgcccc ggggtatcaa cgatgttgat acggtaatca ttccatttga
    20101 tagcggtgtt tttcgcgagg atggtaatcc cacgctcttt ctccaaatcg ttggagtcca
    20161 tcacgcgctc ttgggtttcg gcacgagagt cgaacgtacc ggattgttgg agcagcttgt
    20221 ctaccagggt ggttttacca tggtctacgt gcgcgatgat ggcgatatta cgcaattttt
    20281 cgatcacaac tttgcctcag gcattagaaa tagcgcgtta ttgtacacgg attaatcgca
    20341 ctacaaaaca ggatcacaaa catcctccgc aaacaagtat tgcagagtcc ctttgtgatc
    20401 gctttcacgg agcataaaaa gggttatcca aaggtcattg caccaacatg gtgcttaatg
    20461 tttccattga agcactatat tggtgcaaca ttcacatcgt ggtgcagccc ttttgcacga
    20521 tggtgcgcat gataacgcct tttaggggca atttaaaagt tggcacagat ttcgctttat
    20581 cttttttacg gcgacacggc caaaataatt gcagatttcg ttaccacgac gaccatgacc
    20641 aatccaggag agttaaagta tgtccgctga acacgtactg acgatgctga acgagcacga
    20701 agtgaagttt gttgatttgc gcttcaccga tactaaaggt aaagaacagc acgtcactat
    20761 ccctgctcat caggtgaatg ctgaattctt cgaagaaggc aaaatgtttg acggctcctc
    20821 gattggcggc tggaaaggca ttaacgagtc cgacatggtg ctgatgccag acgcatccac
    20881 cgcagtgatt gacccgttct tcgccgactc caccctgatt atccgttgcg acatccttga
    20941 acctggcacc ctgcaaggct atgaccgtga cccgcgctcc attgcgaagc gcgccgaaga
    21001 ttacctgcgt tccactggca ttgccgacac cgtactgttc gggccagaac ctgaattctt
    21061 cctgttcgat gacatccgtt tcggatcatc tatctccggt tctcacgttg ctatcgacga
    21121 tatcgaaggc gcatggaact cctccaccca atatgaaggt ggtaacaaag gtcaccgtcc
    21181 ggcagtgaaa ggcggttact tcccggttcc gccggtagac tctgctcagg atattcgttc
    21241 tgaaatgtgt ctggtgatgg aacagatggg cctggtggtt gaagcccatc accacgaagt
    21301 agcgactgct ggtcagaacg aagtggctac ccgcttcaat accatgacca aaaaagctga
    21361 cgaaattcag atctacaaat atgttgtgca caacgtagcg caccgcttcg gtaaaaccgc
    21421 gacctttatg ccaaaaccga tgttcggtga taacggctcc ggtatgcact gccacatgtc
    21481 tctgtctaaa aacggcgtta acctgttcgc aggcgacaaa tacgcaggtc tgtctgagca
    21541 ggcgctgtac tacattggcg gcgtaatcaa acacgctaaa gcgattaacg ccctggcaaa
    21601 cccgaccacc aactcttata agcgtctggt cccgggctat gaagcaccgg taatgctggc
    21661 ttactctgcg cgtaaccgtt ctgcgtctat ccgtattccg gtggtttctt ctccgaaagc
    21721 acgtcgtatc gaagtacgtt tcccggatcc ggcagctaac ccgtacctgt gctttgctgc
    21781 cctgctgatg gccggtcttg atggtatcaa gaacaagatc catccgggcg aagccatgga
    21841 caaaaacctg tatgacctgc cgccagaaga agcgaaagag atcccacagg ttgcaggctc
    21901 tctggaagaa gcactgaacg aactggatct ggaccgcgag ttcctgaaag ccggtggcgt
    21961 gttcactgac gaagcaattg atgcgtacat cgctctgcgt cgcgaagaag atgaccgcgt
    22021 gcgtatgact ccgcatccgg tagagtttga gctgtactac agcgtctaat agttgaagtt
    22081 gtactacccg gcgcaacaac gccggaggtt caaaccaggc ccatctggcg taattgttgc
    22141 agccagtttg aacacggaca gcgcgcagaa cccggagcgt acactggtac gtgaggagtt
    22201 cgagcactgc ccaggttcaa aatggcaaat aaaatagcct gatgggactg gtttttagtt
    22261 gccgtggaaa tttcagccca tctctgcatg ggcttttttc tccgtcaatt ctctgatgct
    22321 tcgcgctttt tatccgtaaa aagctataat gcactaaaat ggtgcaacct gttcaggaga
    22381 ctgctttatg gcaacaggca cgcagcccga tgctgggcag atcctcaact cgctgattaa
    22441 cagtattttg ttaatcgatg acaacctggc gatccattac gccaaccctg ccgcgcaaca
    22501 actgctcgcc caaagctccc gcaaattgtt tggtacaccg ttaccggaac tgttgagcta
    22561 cttctcatta aatatcgagc tgatgcaaga aagtctggag gcggggcaag gttttaccga
    22621 taacgaagtg acgctggtca tcgacgggcg ctcgcatatc ctttctgtga cggcccagcg
    22681 tatgccggac ggcatgatcc tgctggagat ggctccgatg gataaccagc gccgcttaag
    22741 tcaggaacag ctacagcacg cccagcaggt tgctgcccgt gatttagtgc gcggcctggc
    22801 acatgagatt aaaaatccgc ttggcggttt acgtggcgcg gcgcagctac tcagcaaagc
    22861 gttacctgac ccatcactac tcgaatatac caaagtgatt atcgaacagg cggaccggct
    22921 gcgaaatctg gtcgaccgtc tgttggggcc gcagctgccc ggtacgcgcg ttaccgaaag
    22981 tattcacaaa gtagctgaac gcgtggtaac gctggtgtcg atggaactgc cggacaacgt
    23041 gcggttgatt cgtgattacg atcccagcct gccggaactg gcgcacgacc cggatcaaat
    23101 tgaacaggtc ttgctgaata ttgtgcgcaa tgcgctacag gcgctggggc cggagggtgg
    23161 tgaaatcatt ctgcgtaccc gcaccgcgtt tcaactgacc ttacacggcg agcgctaccg
    23221 gctggcggcg cggattgatg tggaagataa cgggccgggc attccgcctc atttgcagga
    23281 tacgctgttt tacccgatgg tcagcggccg cgaaggtggc accgggcttg gcttatccat
    23341 cgcccgtaat ttgattgatc agcattcagg caaaattgaa tttaccagtt ggccaggtca
    23401 taccgagttc tcggtttacc tgcctatcag gaaataaagg tgacgtttat gcaacgaggg
    23461 atagtctggg tagtcgatga cgatagttcc atccgttggg tgcttgaacg tgcgctcgct
    23521 ggagcgggtt taacctgtac gacatttgag aacggcgcgg aagtactgga ggcgctggcg
    23581 agcaaaacgc cggatgtgct gctttcagat atccgtatgc cgggaatgga cgggctggcg
    23641 ctgctcaagc agattaaaca gcgccatcca atgcttccgg tcatcattat gaccgcacat
    23701 tccgatctgg atgctgccgt cagcgcctat caacaagggg cgtttgatta tctgcccaaa
    23761 ccgtttgata tcgacgaagc cgtcgcgctg gttgagcgcg ccatcagtca ttaccaggaa
    23821 cagcagcagc cgcgtaatat tcagcttaac ggcccaacga ccgatatcat cggcgaagcg
    23881 ccagccatgc aggacgtgtt ccgtattatc ggtcggcttt cgcgttcttc tattagcgtg
    23941 ctgattaacg gcgaatccgg caccggtaaa gaactggtcg ctcatgccct gcatcgccac
    24001 agtccgcgcg ccaaagcgcc gtttatcgcg ctgaatatgg cagctatccc aaaagatttg
    24061 atcgaatcag aactgtttgg ccacgagaaa ggcgcgttta ctggcgcgaa taccattcgt
    24121 caggggcgtt ttgaacaggc cgatggcggt acattattcc tcgacgaaat tggtgatatg
    24181 ccgctggatg tgcagacgcg tttgctgcgc gtgctggcag acggtcagtt ttaccgcgtt
    24241 ggcggctatg cgccggtgaa agtggatgtg cggattatcg ctgccactca ccagaatctc
    24301 gaacagcgag tgcaggaagg taagttccgt gaggatctgt tccaccgcct gaacgttatc
    24361 cgcgttcatc tgccgccgct gcgcgaacgt cgggaagata ttccccgtct ggcgcgccat
    24421 tttttacagg ttgccgcgcg cgaactgggc gtagaagcga agttactgca tccggaaacc
    24481 gaagctgctc tgacgcgtct ggcgtggcca ggcaacgtgc gccagctgga aaacacctgc
    24541 cgctggctaa cggtgatggc cgccgggcag gaagtgttga ttcaggattt gcccggcgaa
    24601 ctgtttgaat caacggttgc ggagagtact tcgcaaatgc aaccggacag ctgggcgacg
    24661 cttcttgcgc agtgggcaga cagagcgctg cgttccggtc atcaaaatct gctttccgaa
    24721 gcgcagccag agctggagcg gacgttactg acgaccgcgt tgcgacatac gcaggggcat
    24781 aaacaggaag cggcgcggct actcggctgg ggccgcaaca ccctgacgcg taagttaaaa
    24841 gagctgggga tggagtgatt cacggcgtgt gtgtaaagat tgattattga gcgcaaattg
    24901 ctggtatttt acgctttact gttccgataa gttcagtatg atcttgcccg gaaaacgggg
    24961 agagtcatta tgctggaatc aataattaat ctggtatcga gtggcgcagt tgacagccac
    25021 acaccgcaaa ctgccgttgc tgccgtgctg tgtgccgcaa tgattgggct gtttagttga
    25081 gtttcagatt gctgatgaag tgctctttgt tcatgccgga tgcggcgtga acgccttatc
    25141 cggcctacaa aagcaatatt ttcaacaggt tttgtaggcc tgataagcgt agcgcatcag
    25201 gcaatcttgt tctcagcaca ttgatggcgc tttcgttttc tactttgtaa acgaagcgcc
    25261 attcactatt taaatcaccc gtgagaactg ctgcatccgc gctttctggc gcagataggt
    25321 atcaaagcac atgcaaatgt tgcggatcag caagcgacct ttcgccgtca cctgtattcc
    25381 cttctcatcc acatccacca gcccatcttt tgctaacggg gcgagcagct tgagatcttc
    25441 cgcaaagtaa tcagcgaagt gcaaatccca ctgtttctca ataggggcgt aatccagacg
    25501 gaagttgcag atgagcgact taatcacatc gcggcgaata cagtcatcac gcgttagcgc
    25561 aataccacgc cacagcgcat tgccttgttc atccacttgc tgatagtact gcttcaactc
    25621 tttctggttc tgcgcgtagc agtcgccaat catgctgatg gcggaaacgc ccatccccag
    25681 cagatcggta tcgccctgag tggtgtagcc ctggaagtta cgatgcagca cgccttcacg
    25741 ctgggccacc gccagctcgt catccggacg ggcaaagtga tccataccga taaactgata
    25801 gcccgattgc gtcaggaagg cgatggtttc ctgcaggata tcgagttttt gctgcggact
    25861 cggcaggtca gcatctttga ttttgcgctg agcagcaaaa atggtcggca gatgcgcgta
    25921 gttaaagaca ctcagacgat cggggttcag ctccgccaca cgtttcaggg taaaggcgaa
    25981 actctccggc gtctgtttcg gcaggccgta aatcaggtcg atgttggtgg aggtaaaacc
    26041 aatctcacgc gcatggttaa gcagtgcaaa gatgaactct tcatcctgct cgcggttaac
    26101 cagacgttgc acttctttgt tgaagtcctg cacgcccatg ctcaggcgat taaagccttc
    26161 ggcgcgtaaa tgatcgagta catccagttc gatttcccgc ggatcgactt cgatcgaaat
    26221 ctccgcatcg gcattgaact ggaagttttc gcgcagcagc ttcatcaggc ggctgatttg
    26281 cgctttattc agatacgtcg gcgttccgcc gccccagtgc aattggctga cgtgacgccc
    26341 ggcgaacagc ggtgcacgat ggacgatttc ttgctccagc gcgtccagat actgatcggc
    26401 cttgtgctgc tggcgagtaa caatcttatt gcaaccgcag aagtaacaaa gcttatggca
    26461 gaacgggata tgtacgtaga gagataatgg acgctcagga tagcgcgcca cggcttgtaa
    26521 aaacgcctgt tcgccgaagt cttctgaaaa ctccagcgcg gtcgggtacg aggtgtatcg
    26581 tggcccggaa tagttatatt tctggatcag ggccagatcc cagtcgattt gctgtacaga
    26641 catgctcact ccttccgatg gcgtctctgg cggctacggc gtaccggcgg atgcccgttt
    26701 cgggtcatca gccggttgcg cagccacttt tgtcgccgcg acaaccgctg tagtttaacg
    26761 aataaccaca ccagataaca tataaccgga agggttatca gcagaacggg aaggcccacg
    26821 gcggcaatcc gttagttgcc ccgcagcaga cgcatcatgt cttcttgctt ctcgtcttct
    26881 tcctcttctt cgtcatcgtc ataagagagg ccgagtttct gcatcaactc atcaatacga
    26941 tccagtttag catccaccca ggattgctct tcggcgctca gggtttcgcc tgcttccaga
    27001 cgttccagca gcgcatccag acgctcatcc gtttccagta attccaactc cgcctgcggt
    27061 gaaagcatag gtttctcact cttcggtttg tgctgtttgg tgactttttc aatcacgccc
    27121 aatggaatag gggttttact gccaatacgt ggatcttttg gtgcgttctg gcctttgctg
    27181 cctgacgtgg tgtttccgcc cgctgcgcgg ctgcccggcg catgaccacg acgttttttc
    27241 tgacgcttgc gatcacgagc ttcctgatcc agctcctcgc gtgtttttcg acgcgctttt
    27301 gcatgacctt tgctgcgtga gtttgaagat gatggtttca taatggttcg tcttttagcc
    27361 gttttattca gtatagattt gcggcggaat ctaacagaaa gcaagcaaag aaaaaaggcg
    27421 acagattact ctgtcgcctt ttttcctgac tcataaccct taacgggtct tacaatcctt
    27481 gcaggccgcc aacacaccct gtttttcctt tagccggaac aatccgtgtt gattccattt
    27541 ccgtttaatt acgtctccgg acgtttgtct tcctgacaat cctctgtctt cgcctcctgg
    27601 cgctcctgac cctcatcctg agtcattgtt cctgttagcg tcctcgctct atgggtgcta
    27661 ctttacgcct ttgctttaaa caaacaatca accgccatca gtatttgcac attttcagag
    27721 ttttatataa aagcaattca ctgatttata tagcttattt gacatgcatc gcagaatttt
    27781 ctctgctatt tcgcataaag actatcggca atgtctcaca aagcgtatgg cttttactta
    27841 caacgctcac gccgaaaaat ccgcctttta ttatggttca ggtataatcg ccacaaagct
    27901 ttgactgacg gagacgaccg ctttgactaa tttgaattat caacagacgc attttgtgat
    27961 gagtgcgcct gatattcgcc acctaccttc cgataccgga attgaagtgg cttttgcagg
    28021 ccgttccaac gcaggtaaat ccagcgcgct gaacacgctg actaaccaga aaagcctggc
    28081 tcgtacctca aaaaccccag ggcgcaccca gcttatcaac ctgtttgaag tggctgacgg
    28141 caagcgtctg gttgacttgc ctgggtacgg ttatgcggaa gtcccggaag agatgaagcg
    28201 caaatggcag cgtgcgctcg gcgaatacct cgaaaaacgt cagagcctgc aaggtctggt
    28261 ggtgctaatg gatattcgcc atccgctgaa agatttggat cagcagatga ttgagtgggc
    28321 ggtagacagc aatatcgccg ttctggtgct gctgaccaaa gcggacaaac tggcaagcgg
    28381 cgcacgtaaa gcgcaattga atatggtgcg tgaagctgta ctggcgttta acggtgatgt
    28441 gcaggttgaa acgttttctt cgttgaagaa acaaggcgtg gacaagctgc ggcagaaact
    28501 ggatacctgg tttagcgaga tgcagcctgt agaagaaacg caggacggcg aataattttc
    28561 ttgccttaat gcttgtgccg gatgtggcgt atccggcccg taaattcaat agattacaat
    28621 tctaccgttg gcctgataaa catagcgcat caggcattac ggatcttttc tttcgcccaa
    28681 taaaaaacgc cccagtcatt actgactggg gcggctaaaa tattcagcca aatccgatta
    28741 cgtgaagtaa aaggtctgaa agatagaaca tcttacctct gtaccctacg cgactaactt
    28801 tactcttttt tgttcagttc agaaagcact ttttgtaatt ttttttcagt tgttgcatag
    28861 gaatttctaa tagccatcac aaaacgtgac agcttatgtt gcttacttac gaaaaaaggg
    28921 catgttcagg cgaatcttag tgcgcctgat cccagttttc gccactcccc acttccacca
    28981 gcaacggcac atccagacgg gtacagtttt ccatcagttg atgaatctgc ttcgcgacgg
    29041 catcaacatc atctttatga acttcaaata ccagttcatc gtgtacctgc atgatcatac
    29101 gtacacgcgg ttgctcagcc tgtaaccacg catcaacggc aatcatcgcc cgtttgataa
    29161 tgtcggcggc ggttccctgc attggcgcgt taatggctgc acgttcagcc gctgcacgac
    29221 gagcaccatt gctggatttg atatccggca gatacagacg gcgtccgtcc agcgtttcaa
    29281 cgtagccctg ctctttcgcc tgagcacggg tgcgttccat atactccagc acgccagggt
    29341 agcgttcgaa gtaaaggtcc atgtacttct gcgcttcttt acgtggaatg ttcaattgcc
    29401 gcgccagacc gaaagcactc atgccataaa tcagaccaaa gttgatcgct ttcgcgctac
    29461 ggcgttgctc gctggtgacg gtttccagtg gcaaaccaaa cacttctgcc gccgttgccc
    29521 ggtggatatc ttttccttcc gcgaatgcgg tcagcaagcc tttgtcacgc gagagatgcg
    29581 ccataatgcg cagttcaatc tgcgagtagt ccgctgagac aatcacataa tcctctggcg
    29641 caataaacgc ctggcggata cgacgacctt cttcgttacg caccggaatg ttttgcaggt
    29701 taggatcggt tgacgataaa cgtcccgttg cggttactgc ctggtgataa gaggtatgca
    29761 cacgcccggt tttcgggttg atcatcagcg gcagcttgtc ggtgtaagtc gacttcagct
    29821 tcgccagacc acgatactcc agaatcactt ttggcaacgg atagtccagc gccagttctt
    29881 ccagtacctc ttccgacgtt gacggcgcgc cacccggcgt tttcttcagc ggtttaatac
    29941 cctgtttttc aaacagaatg gtttgcaact gcttggtgga agaaaggtta aattcttcac
    30001 ctgcaatttc atgcgctttc ttttccagct cagccagacg caaggtgagc tcttcagaat
    30061 gattgtgcag cactttcgga tcgatcttca caccgttacg ttcaatgcgt gaaagcactg
    30121 gcaccagcgg catttcgata ttctcgaaga cgttcaacgg ccctttgtgt ttttgcagat
    30181 ccggccacat tttcagatgc aactgcaagg tgacatcagc atcttcggcg gcgtaacgtc
    30241 cggcctcttc gagggcaatc tggttaaagg tcagttgatt tttgccttta cccgcaatct
    30301 cttcaaaagt gatggttttg tgcttcaacc aacgttccgc gaggctgtcc atatcgtgac
    30361 gcccggcaac gctattgaga atgtaggact ccagcatggt atcaaacgca atcccacgca
    30421 gctcaatgcc gtaattcgcc agaataccgc gatcgtattt caggttttgc ccgaccttca
    30481 gcgccttttc atcttccagc agcggtttta gcaactcgag tgcacgctcg cgagagattt
    30541 gatcgggcgc gtcaagataa tcatgagcaa ccggaatata tgccgctacg cctggctcga
    30601 tagcaaaaga aagcccgacc aggttagcag agatgttatc aaggctgtcg gtttcggtat
    30661 caaatgcaaa taccggcgct ttttccagct tcgcaatcca cgctttcagt gtttcttcat
    30721 caaggatggt gacgtagttg tcataagaaa tcaccgttgc cgtcacttct ggcgcttcgt
    30781 ctgcaacact ggtttcctgc ggcttcgcgg ctggttttgc ccctttggcc tgtaaccatt
    30841 tgcccgcttc gacatcagca gtccagcgtt tgaactcata ctttttgaac agccccaaca
    30901 actcttccgc tgccggttgc tgcacttcca gttgttcaca ggtcagctcc agttcaacgt
    30961 cggttttaat cgtcgccagc tggtatgaga gataagcaac ttctttgttt tgctcgagct
    31021 tcgctgccat tgttttcgcg ccacggaagc tcaacccagc aattttttct ggctcggcat
    31081 acagcgtatc cagcccgcca agaccttgca gcaatgcctg cgcggttttt tcaccgacgc
    31141 ccggtacgcc aggaatgtta tcggaggagt cacccatcag cgccaggaaa tcgatgatca
    31201 gttctggcgg cacgccgtac ttattcacca cctcttccgg tccgaggatg gtattcgtca
    31261 tggtattgat aagcgtaata tttggcgtca ccagctgcgc catatcttta tcgccagtgc
    31321 tgatcagcac cggacgcccg gctttttcgg cttcgcgcgc cagagtaccg ataacgtcgt
    31381 ccgcttctac gccagaaacc gccagcagcg gcagtcccat cgctttaacc atcgcgtgca
    31441 ggggttcgat ttgtgctcgc agatcgttcg gcattggcgg gcgatgtgat ttgtaatgtt
    31501 caaacagttc atcacgaaag gtttttccct tggcgtcaaa gaccaccgct gcatgcgtcg
    31561 gtttatattg catgatcaga ctgcgcagca tgttgaggac accatacatc gcaccggtcg
    31621 gctcgcctgc gctgttagtc agcgggggaa acgcgtgata tgcgcgataa agataagatg
    31681 aaccatctac aaggataagt ggattttggg ggatctgaac cataatgtcc gtgcctgttt
    31741 atcagattat gggtaaagga tgccacagac aggatgaaaa catgagtttt tcaccgcttt
    31801 tcgtgaaaat cttttgtaga tcttctggat cgctcgcaaa tcgtcatgtg gataactttg
    31861 tgcataaatt atttacatcg cttttaattt attatcatgc tttttacagc atgatgtttg
    31921 tctttgtttt
//
LOCUS       DBANUU010000047        31793 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000047 DBANUU010000000
VERSION     DBANUU010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.047"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(189..1562)
                     /gene="argH"
                     /locus_tag="ACIE68_002074"
     CDS             complement(189..1562)
                     /gene="argH"
                     /locus_tag="ACIE68_002074"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFX5908386.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1623..2399)
                     /gene="argB"
                     /locus_tag="ACIE68_002075"
     CDS             complement(1623..2399)
                     /gene="argB"
                     /locus_tag="ACIE68_002075"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFX5908387.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(2407..3411)
                     /gene="argC"
                     /locus_tag="ACIE68_002076"
     CDS             complement(2407..3411)
                     /gene="argC"
                     /locus_tag="ACIE68_002076"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFX5908388.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3565..4716
                     /gene="argE"
                     /locus_tag="ACIE68_002077"
     CDS             3565..4716
                     /gene="argE"
                     /locus_tag="ACIE68_002077"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFX5908389.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            5068..7719
                     /gene="ppc"
                     /locus_tag="ACIE68_002078"
     CDS             5068..7719
                     /gene="ppc"
                     /locus_tag="ACIE68_002078"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFX5908390.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7902..9635
                     /gene="eptC"
                     /locus_tag="ACIE68_002079"
     CDS             7902..9635
                     /gene="eptC"
                     /locus_tag="ACIE68_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFX5908391.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSKQTDKQFYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9784..10635
                     /gene="yijO"
                     /locus_tag="ACIE68_002080"
     CDS             9784..10635
                     /gene="yijO"
                     /locus_tag="ACIE68_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFX5908392.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10622..10963)
                     /gene="frwD"
                     /locus_tag="ACIE68_002081"
     CDS             complement(10622..10963)
                     /gene="frwD"
                     /locus_tag="ACIE68_002081"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFX5908393.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10965..11843)
                     /gene="pflC"
                     /locus_tag="ACIE68_002082"
     CDS             complement(10965..11843)
                     /gene="pflC"
                     /locus_tag="ACIE68_002082"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFX5908394.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11809..14106)
                     /gene="pflD"
                     /locus_tag="ACIE68_002083"
     CDS             complement(11809..14106)
                     /gene="pflD"
                     /locus_tag="ACIE68_002083"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFX5908395.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14157..14477)
                     /gene="frwB"
                     /locus_tag="ACIE68_002084"
     CDS             complement(14157..14477)
                     /gene="frwB"
                     /locus_tag="ACIE68_002084"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFX5908396.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14492..15571)
                     /gene="frwC"
                     /locus_tag="ACIE68_002085"
     CDS             complement(14492..15571)
                     /gene="frwC"
                     /locus_tag="ACIE68_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFX5908397.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLISRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15880..18381
                     /gene="ptsP"
                     /locus_tag="ACIE68_002086"
     CDS             15880..18381
                     /gene="ptsP"
                     /locus_tag="ACIE68_002086"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFX5908398.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18393..19055
                     /gene="fsa"
                     /locus_tag="ACIE68_002087"
     CDS             18393..19055
                     /gene="fsa"
                     /locus_tag="ACIE68_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFX5908399.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            19066..20169
                     /gene="gldA"
                     /locus_tag="ACIE68_002088"
     CDS             19066..20169
                     /gene="gldA"
                     /locus_tag="ACIE68_002088"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFX5908400.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            20444..21061
                     /gene="yijF"
                     /locus_tag="ACIE68_002089"
     CDS             20444..21061
                     /gene="yijF"
                     /locus_tag="ACIE68_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFX5908401.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21088..21993)
                     /gene="yijE"
                     /locus_tag="ACIE68_002090"
     CDS             complement(21088..21993)
                     /gene="yijE"
                     /locus_tag="ACIE68_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFX5908402.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRAMRPTPFKYTLAIALLQTCGMIGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALVYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGVVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22087..24267)
                     /gene="katG"
                     /locus_tag="ACIE68_002091"
     CDS             complement(22087..24267)
                     /gene="katG"
                     /locus_tag="ACIE68_002091"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFX5908403.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24596..25486)
                     /gene="metF"
                     /locus_tag="ACIE68_002092"
     CDS             complement(24596..25486)
                     /gene="metF"
                     /locus_tag="ACIE68_002092"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFX5908404.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            25562..25696
                     /locus_tag="ACIE68_002093"
     CDS             25562..25696
                     /locus_tag="ACIE68_002093"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908405.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(25835..28267)
                     /gene="metL"
                     /locus_tag="ACIE68_002094"
     CDS             complement(25835..28267)
                     /gene="metL"
                     /locus_tag="ACIE68_002094"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFX5908406.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28270..29430)
                     /gene="metB"
                     /locus_tag="ACIE68_002095"
     CDS             complement(28270..29430)
                     /gene="metB"
                     /locus_tag="ACIE68_002095"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFX5908407.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29707..30024
                     /gene="metJ"
                     /locus_tag="ACIE68_002096"
     CDS             29707..30024
                     /gene="metJ"
                     /locus_tag="ACIE68_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFX5908408.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30208..30816
                     /gene="yiiX"
                     /locus_tag="ACIE68_002097"
     CDS             30208..30816
                     /gene="yiiX"
                     /locus_tag="ACIE68_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFX5908409.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            30961..31636
                     /locus_tag="ACIE68_002098"
                     /pseudo
     CDS             30961..31636
                     /locus_tag="ACIE68_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         7688 a         8847 c         8049 g         7209 t
ORIGIN      
        1 aggtaaagaa gacacaatga taaaaaggaa tgtgacaaat aatggttctg gttgggcgtt
       61 aaatttaagc tcaccagaat gctgacaaat ttctgcctga tgtggcgtaa acgccttatc
      121 cgcctacgaa gtgtcgcact ttgtaggcgt ggtaagctcc ccattttcag gggagcaata
      181 taaacaaatt actctaaccg cgcctgcgca aaagcaattg cctgcgccac ctgctgcggt
      241 gagacgccgc cttttgccgc acgcttgtcg aggcacgatt gcagcgacag aatcggatag
      301 acatcttcgt caatcaccgg gctgaatttc tgcaactcgt cgagcggcag atcttccagc
      361 ggtttgccct gacgaatggc ttccaccacc gcttcaccaa caatatggtg cgcctcgcgg
      421 aacggtacgc ctttcgccac cagataatcc gccagttcgg tggcgttggc gtaaccctgc
      481 tgagccgctt cctggcaacg tggacgtttc acctgaatgc cgtccagcac cagcgccgcc
      541 atatgcaggc agtccagcca ggtatcgagc gcgtcgaaca gaccttcttt gtcttcctgc
      601 atatctttgt tgtaagccag cggcaaacct ttcagcgtca tcatcatgcc ggttaacgcc
      661 ccctgcaccc gaccgcattt accgcgaatc agctccagcg catccgggtt tttcttctgc
      721 ggcattaatg atgaaccgga agtcacgcgg tcagaaagct ccacaaaccc cgcttcgccg
      781 gtgttaaaga aaatcagatc ttcagcaaaa cgcgacagat gcaccatgcc gatagcggca
      841 gcagaaagca gttccaacac atggtcacgg tcagaaacgc tgtcgagact gttacgggtc
      901 gccgaagcaa agcccagcca gcctgctaac tgttcacggt cgatttcata ggccgttccc
      961 gccagcgcgc cacagcctag cgggctgaca tccagacgct taagcgcatc ctgcaaacgg
     1021 ctttcatcac gcgccagcat ctcaacatag gccaggcacc agtgcgcgaa cgtcaccggc
     1081 tgggcgcgtt gcaggtgagt gtaacctggc attaccgcgt cctgattgtt ttgtgcggtt
     1141 tccaccagcg ccgattgcag ctgccggtta gccgtcagta actcgctaac ggtatctttg
     1201 caccacagtt tcaggtcagt cgctacctga tcattacggc tacgcccggt atgcagcttt
     1261 ttgcctaact ggcccacttt gtcgatcagt ttgccttcca cccagctatg gatatcttcg
     1321 gcgtcgcttt caaggatttg ttgtggcctg gcgcgaacat cttccagcaa cacgttcagc
     1381 gcctcttcca gttgcgcctg ctcttctgcg gttaacacgc ctaccgtgac cagggctttg
     1441 gaccaggcca cagagccaac aatatcctgc tccgccagac ggtaatcaaa gcgcagtgag
     1501 tcgttgaatt gtttgaaccg ttgatctgct gcctgggtaa aacgcccgcc ccaaagtgcc
     1561 ataactctgt ttccttattt tgaaattcaa tgccggaaag ctgcgcctcc ggccaacaaa
     1621 acttaagcta aaatccgcgt acccatcggc ataccgttaa acagtgccgg aagctgctcc
     1681 gcatgacgcc aggaggcgat atctaccgga cggcccagcg tgcgggccgc atccagcgcc
     1741 gcgttcactt tcactatcat gccgtcagta ataatgccct gctcaatcag ttgttctgct
     1801 ttcgcggcgg tcatttcggc aatgcgttgc cctttgccgt cgagaatgcc gctgacgtcg
     1861 gagagcaaaa tcagatccgc gcccagcgtt gccgccagcg ccgttgccgc ctggtcggca
     1921 ttgacgttca tcagttgccc ttcgtctgtt acgccaatgg agctgaccac cggcagataa
     1981 ccgttctcca gcaaggagtt gataagctta ggcgaacctg gctgcgccag tccaacatga
     2041 cctaactctt catcaagctg ggtcactttg acgctgtcgc cgtcaccgag aaacaaacct
     2101 acggccgcaa tctgatgttt cttcgcccat gccaacaggg ttttatttgc cgttcccgcc
     2161 agtgctccgg tgataatgtc tatctgatca gcaggcgtca cccgcaggcc gtttttcttt
     2221 ttcaccggca gattcagccc tttcatcagc tcatccacca cgcaaccgcc gccgtgcaca
     2281 atcaccagcg gacgctgatg tgactcacga taattcacca gtgcgctaaa cagacgttcc
     2341 agcgcctctt cactatccag cagtacgccg cccagtttga taattaatgg attcatcatt
     2401 gcacccttaa ataagagact gcgtttccgc atagccgaaa cgaatattgg cgcactgtac
     2461 cgcttgtgcc gccgcgcctt tcagtaagtt gtcttcggtc gccacaataa tcagatgctc
     2521 gccctgaacg gcaaacccga tatcgcaaaa tggcagccca acgacatttt tcagcgccgg
     2581 aacgcctttg tcatacagcc gcaccagcgg tttatgggca tacgcctgct gtaacacttg
     2641 cgcgacttgc gcctgggtca cacccgattt caggcggcag gtaatggttt cgagaatgcc
     2701 gcgcgggaaa ttgcccagat gtggggtgaa gataacgtca gcaccgaggt gtgtggcgat
     2761 ctctggttga tggcgatgag taaagacgcc atacggttgc aggctaactt cacaaaagct
     2821 gtttgaaatg gccgctttac gccctgcacc gctcacgccg ctggtggcgt tgatcaccgg
     2881 ccactgattg aggtcaagaa gatcggcatc aatcaacggt ttcagcgcca gctgtgccgc
     2941 cgtcggataa cagcccggca ccgcaatcaa attcgcttct tttaatttat taccgcacca
     3001 ctccgccaga ccgtaggctg cctgttccaa cagttccggg tattgatggg taaagccgta
     3061 atatttttca tagaaggtgg cgtcgttaac acgaaacgcg ccggaaaggt cgaacaccac
     3121 gcagcccgct tcaagaaact gcggcgctaa atcgtggcta acttcatggg cggtggcgag
     3181 aaacactacg tccacccctg ggctaaactc gctgatatcc gacatcggct gcaacggcag
     3241 atcaacgatg ccttttagct gcggatgcaa atcggagatt aactttcccg catcattgct
     3301 ttgcgctgaa acagtcaaag cggttatgtt catatgcgga tggcgattta cataggtcac
     3361 tagctctgcg ccagcgtagc cgctggcacc cacaatcagc gtattcaaca tcggggctat
     3421 tcaccttctt atgtctggtt gccaggttaa acgtaaaaca ttcaccttac ggctggtggg
     3481 ttttattacg ctcaacgtta gtgtattttt attcataaat actgcatgaa tattgatact
     3541 atcatgacca gaggtgtgtc aacaatgaaa aacaaattac cgccatttat cgagatttac
     3601 cgcgctctga ttgccacacc ttcaataagc gccacggaag aggcactcga tcaaagcaat
     3661 gcagatttaa tcactctgct ggcggactgg tttaaagatt tgggcttcaa tgtggaagtg
     3721 cagcctgttc caggaactcg caacaaattt aatatgctgg ccagttgcgg acagggggct
     3781 ggcggcttgt tgctggcggg gcataccgat acggtgccat ttgatgacgg tcgctggacg
     3841 cgcgatccgt ttacactgac ggagcatgac ggcaagcttt acggcttagg caccgccgac
     3901 atgaaaggct tttttgcgtt tatccttgat gcgctacgcg atgtcgacgt cacgaaactg
     3961 aaaaaaccgc tctacattct ggcgactgct gatgaagaaa ccagtatggc cggagcgcgt
     4021 tattttgccg aaactaccgc cctgcgcccg gattgcgcca tcattggcga accgacgtca
     4081 ctacaaccgg tacgcgcaca taaaggtcat atctctaacg ccatccgtat tcagggccag
     4141 tcggggcact ccagcgatcc agcacgcgga gttaacgcta tcgaactaat gcacgacgcc
     4201 atcgggcata ttttgcaatt gcgcgataac ctgaaagaac gttatcacta cgaagcgttt
     4261 accgtgccat accctacgct caacctcggg catattcacg gtggcgacgc ttctaaccgt
     4321 atttgcgctt gctgtgagtt gcatatggat attcgtccgc tgcctggcat gacactcaat
     4381 gaacttaatg gtttgctcaa cgatgcattg gctccggtga gcgaacgctg gccgggtcgt
     4441 ctgacggtcg acgagctgca tccgccgatc cctggctatg aatgcccacc gaatcatcaa
     4501 ctggttgaag tagttgagaa attgctcgga gcaaagaccg aagtggtgaa ctactgtacc
     4561 gaagcgccgt ttattcaaac gttatgcccg acgctggtgt tggggcctgg ctcaattaat
     4621 caggctcatc aacctgatga atatctggaa acacggttta tcaagcccac ccgcgaactg
     4681 ataacccagg taattcacca tttttgctgg cattaaaacg taggccggat aaggcgctcg
     4741 cgccgcatcc ggcactgttg tcaaactcca gtgccgcaat aatgtcggat gcgatacttg
     4801 cgcatcttat ccgacctaca cctttggtgt tacttggggc gattttttaa catttccata
     4861 agttacgctt atttaaagcg tcgtgaattt aatgacgtaa attcctgcta tttattcgtt
     4921 tgctgaagcg atttcgcagc atttgacgtc accgctttta cgtggcttta taaaagacga
     4981 cgaaaagcaa agcccgagca tattcgcgcc aatgcgacgt gaaggataca gggctatcaa
     5041 acgataagat ggggtgtctg gggtaatatg aacgaacaat attccgcatt gcgtagtaat
     5101 gtcagtatgc tcggcaaagt gctgggagaa accatcaagg atgcgttggg agaacacatt
     5161 cttgaacgcg tagaaactat ccgtaagttg tccaaatctt cacgcgctgg caatgatgct
     5221 aaccgccagg agttactcac caccttacaa aatttgtcga acgacgagct gctgcccgtt
     5281 gcgcgtgcgt ttagtcagtt cctgaacctg gccaacaccg ccgagcaata ccacagcatt
     5341 tcgccgaaag gcgaagctgc cagcaacccg gaagtgatcg cccgcaccct gcgtaaactg
     5401 aaaaaccagc cggaactgag cgaagacacc atcaaaaaag cagtggaatc gctgtcgctg
     5461 gaactggtcc tcacggctca ccctaccgaa attacccgtc gtacactgat ccacaaaatg
     5521 gtggaagtga acgcctgctt aaaacagctc gataacaaag atatcgctga ctacgaacac
     5581 aaccagctga tgcgtcgcct gcgccagtta atcgcccagt catggcatac cgatgaaatc
     5641 cgtaagctgc gtccaagccc ggtagatgaa gccaaatggg gctttgccgt agtggaaaac
     5701 agcctgtggc aaggcgtacc aaattacctg cgcgaactga acgaacaact ggaagagaac
     5761 ctcggctaca aactgcccgt cgaatttgtt ccggtccgtt ttacttcgtg gatgggcggc
     5821 gaccgcgacg gcaacccgaa cgtcactgcc gatatcaccc gccacgtcct gctactcagc
     5881 cgctggaaag ccaccgattt gttcctgaaa gatattcagg tgctggtttc tgaactgtcg
     5941 atggttgaag cgacccctga actgctggcg ctggttggcg aagaaggtgc cgcagaaccg
     6001 tatcgctatc tgatgaaaaa cctgcgttct cgcctgatgg cgacacaggc atggctggaa
     6061 gcgcgcctga aaggcgaaga actgccaaaa ccagaagggc tgctgacaca aaacgaagaa
     6121 ctgtgggaac cgctctacgc ttgctaccag tcacttcagg cgtgtggcat gggtattatc
     6181 gccaacggcg atctgctcga caccctgcgc cgcgtgaaat gtttcggcgt accgctggtc
     6241 cgtattgata tccgtcagga gagcacccgc cataccgaag cgctgggcga gctgacccgc
     6301 tacctcggta tcggcgacta cgaaagctgg tcagaggccg acaaacaggc gttcctgatc
     6361 cgcgaactga actccaaacg tccgcttctg ccgcgcaact ggcaaccaag cgccgaaacg
     6421 cgcgaagtgc tcgatacctg ccaggtgatt gccgaagcgc cgcaaggctc cattgccgcc
     6481 tacgtgatct cgatggcgaa aacgccgtcc gacgtactgg ctgtccacct gctgctgaaa
     6541 gaagcgggta tcgggtttgc gatgccggtt gctccgctgt ttgaaaccct cgatgatctg
     6601 aacaacgcca acgatgtcat gacccagctg ctcaatattg actggtatcg tggcctgatt
     6661 cagggcaaac agatggtgat gattggctat tccgactcag caaaagatgc gggagtgatg
     6721 gcagcttcct gggcgcaata tcaggcacag gatgcattaa tcaaaacctg cgaaaaagcg
     6781 ggtattgagc tgacgttgtt ccacggtcgc ggcggttcca ttggtcgcgg cggcgcacct
     6841 gctcatgcgg cgctgctgtc acaaccgcca ggaagcctga aaggtggcct gcgcgtgact
     6901 gaacagggcg agatgatccg ctttaaatat ggtctgccag aaatcaccgt cagcagcctg
     6961 tcgctgtaca ccggggcgat tctggaagcc aacctgctgc caccacctga gccgaaagag
     7021 agctggcgtc gcattatgga tgaactgtca gtcatctcct gcgatctcta ccgtggctac
     7081 gtacgtgaaa acaaagattt tgtgccttac ttccgctccg ctacgccgga acaagaattg
     7141 ggcaaactgc cgttgggttc acgtccggcg aaacgtcgcc caaccggcgg cgtcgagtcg
     7201 ctgcgcgcca taccgtggat ttttgcctgg acgcaaaacc gcctgatgct ccccgcctgg
     7261 ctgggtgcag gtacggcgct gcaaaaagtg gtcgaagatg gcaaacagag cgagctggaa
     7321 gccatgtgcc gcgattggcc attcttctcg acgcgtctcg gcatgctgga gatggtcttc
     7381 gccaaagcag acctgtggct ggcggaatac tatgaccaac gcctggtaga caaagcactg
     7441 tggccgttag gtaaagagtt acgcaacctg caagaagaag acatcaaagt ggtgctggcg
     7501 attgccaacg attcccatct aatggccgat ctgccgtgga ttgcagagtc tattcagcta
     7561 cggaatattt acaccgaccc gctgaacgta ttgcaggccg agttactgca ccgctcccgc
     7621 caggcagaaa aagaaggcca ggaaccggat cctcgcgtcg aacaagcgtt aatggtcact
     7681 attgccggga ttgcggcagg tatgcgtaat accggctaat cttcctcttc tgcaaaccct
     7741 cgtgcttttg cgcgagggtt ttctgaaata cttctgttct aacaccctcg ttttcaatat
     7801 atttctgtct gcattttatt caaattctga atataccttc agatatcctt aaggaattgt
     7861 cgttacattc ggcgatattt ttttcaagac aggttcttac tatgcattcc acagaagtcc
     7921 aggctaaacc cctttttagc tggaaagccc tgggttgggc actgctctac ttttggtttt
     7981 tctctactct gctacaggcc attatttaca tcagtggtta tagtggcacc aacggcattc
     8041 gcgactcgct gttattcagc tcgctgtggt tgatcccggt attcctcttt ccgaagcgga
     8101 ttaaaattat tgccgcagta atcggcgtgg tgctatgggc ggcctctctg gcggcgctat
     8161 gctactacgt catctacggg caggagttct cgcagagcgt tctgtttgtg atgttcgaaa
     8221 ccaacaccaa cgaagccagc gagtatttaa gccagtattt cagcctgaaa attgtgctta
     8281 tcgcgctggc ctatacggcg gtggcagttc tgctgtggac acgcctgcgc ccggtctata
     8341 ttccaaagcc gtggcgttat gttgtctctt ttgccctgct ttatggcttg attctgcatc
     8401 cgatcgccat gaatacgttt atcaaaaaca agccgtttga gaaaacgttg gataacctgg
     8461 cctcgcgtat ggagcctgcc gcaccgtggc aattcctgac cggctattat cagtatcgtc
     8521 agcaactaaa ctcgctaaca aagttactga atgaaaataa tgccttgccg ccactggcta
     8581 atttcaaaga tgaatcgggt aacgaaccgc gcactttagt gctggtgatt ggcgagtcga
     8641 cccagcgcgg acgcatgagt ctgtacggtt atccgcgtga aaccacgccg gagctggatg
     8701 cgctgcataa aaccgatccg aatctgaccg tgtttaataa cgtagttacg tctcgtccgt
     8761 acaccattga aatcctgcaa caggcgctga cctttgccaa tgaaaagaac ccggatctgt
     8821 atctgacgca gccgtcgctg atgaacatga tgaaacaggc aggttataaa accttctgga
     8881 tcaccaacca gcagacgatg accgcccgca ataccatgct gacggtattt tcgaagcaga
     8941 ccgacaaaca gttctatatg aaccagcaac gtacgcagag tgcgcgtgaa tacgacacca
     9001 acgtgctgaa accattccag gaagtgctga aggacccggc gccgaagaaa ctgatcatcg
     9061 ttcatctgct gggtacgcat atcaaataca aataccgcta cccggaaaat cagggcaagt
     9121 ttgatggcaa taccgatcat gttccgcctg gattaaacgc ggaagagctg gagtcatata
     9181 acgattatga caacgctaac ctgtataacg atcatgtggt tgccagcctg attaaagact
     9241 ttaaagcagc aaacccgaac ggtttcctgg tttatttctc tgaccacggt gaagaggttt
     9301 acgacacgcc gccgcacaaa actcaggggc gtaacgaaga caacccgacg cgccacatgt
     9361 acaccattcc gttcctgctg tggacgtcgg aaaaatggca agcgactcat ccgcgtgatt
     9421 tctcgcagga tgttgatcgt aaatacagcc tggcggaact gatccacacc tggtcagatt
     9481 tagcaggctt atcttacgac ggttacgatc caacccgttc agtggtgaat ccgcagttca
     9541 aagaaactac ccgctggatt ggtaacccgt acaagaaaaa cgcgctgatc gattacgaca
     9601 cgctgccgta tggcgatcag gtgggtaatc agtaaatgac ctttgtatcg cgtctggcgc
     9661 tatttgccgg atgcgatact gccgcaatgt ttacatcgaa ctcccctcag gccacacttt
     9721 tcaggtggcc ttttttaata atgccagtta ctgttttccc gtctacatac ctaagggaat
     9781 aacatgtatc acgacgtcag ctatctgctc tcccgcctga tcaacggccc gctgtccctg
     9841 cgccagattt actttgccag cagtaacggc ccggtgcccg atctcgcgta tcaggtcgat
     9901 tttccacggc tggaaattgt gctggaaggt gagtttgttg ataccggcgc tggagcaacg
     9961 ttagttcccg gcgatgtgct gtacgttcct gctggtggct ggaattttcc acaatggcaa
    10021 gcccccgcta ctacctttag cgtgctgttt ggcaaacagc aactcggctt cagcgtcgtg
    10081 caatgggatg gcaaacaata tcaaaatctg gcgaagcaac acgtcgcccg gcgcggccca
    10141 cgcataggtt cttttctgct acaaacgctc aatgaaatgc aaatgcagcc gcaggagcag
    10201 caaacggcaa ggcttatcgt cgccagcctg cttagccact gccgcgattt gctcggcagc
    10261 caaatccaga ccgcctcacg cagccaggca ctatttgaag ctattcgcga ttatatcgac
    10321 gaacgctatg cctccgcgct tacccgcgaa tctgtcgcac aggcattcta tatttcacca
    10381 aactatctct cgcacctgtt tcaaaaaacg ggggccattg gttttaacga atacctgaat
    10441 cacacgcgac tggagcacgc taagacgtta ttgaaaggct atgacctgaa ggtaaaagaa
    10501 gtggcgcacg cctgcggctt tgtcgacagc aactacttct gccgtctgtt tcgtaaaaac
    10561 accgaacgct cgccgtcgga atatcgtcgg cagtaccaca gccaactgac agaaaaaccg
    10621 actactccag aataagatgg gtttgctgcg gcgcagaaag cactttgcgc accgcgctca
    10681 ttacccgctg cggctcacgc aggaatgcgt agatgctgca ttgcacatag cggcaatgtt
    10741 caaatcgctc ggcaccggca agctcgatat ccgtaatcaa cagagcaaca tccgcccgac
    10801 ggatatcctc gtccgctaaa cgattctccg ttcccagcgc gccctgagtt tcaatgctga
    10861 cgccccactt ctctaactgg cacaactttt ccagccgttc cgccgccata taagtatgcg
    10921 ccacgccgct tacgcaggcg gttactgcca ccaggtatgc cattttaacc tcccacggta
    10981 acctgaaatc cggcccgttc tgccatttcg cgcatcgttg tcacatcggc tgacgacggc
    11041 gcaggcacct ctttcatcga ccatgttttc cccagcaggc ggtatttcgg ttcgccgtac
    11101 tgatgaaacg gtaacagatg gatctgcctg attttcagcg ggatcagtac atccagcgcc
    11161 tgctgcatat tctcccggct gagcgtgaaa ccagggatca acggtaaacg cgggatcacg
    11221 ttgacaccct cactcaccag caaacgcaga ttctccagca cgcgtggcag gttcatcttc
    11281 accacatccc gcgcctgagt cgcgtccata atttttaaat cgaacagcac ttcatcgcac
    11341 aatttcgcca gtggtaacag cttgctggct ggcgcgtctc cggcagtttc aatggcgcat
    11401 gacacgcccc acaaccgcag ccgctgtaaa aaacgggtag caaactccgc ctgcattaac
    11461 acttcgccgc cagaaagcgt gacgccgccg ccggacgtgc gaaagaaaat gtcatctttc
    11521 atcacttccc gttccagcgc gtcaaggctg atatcgcgac caatccgttc aaacgccccg
    11581 gaggggcatt catccgcatc acgcaaacat ttcgcacagt gcagacattt cgcctctctg
    11641 cgtaccgtct ggattttgcc ggagatcgac tccggattag cacaccatgg gcaaagatgc
    11701 ggacagcctt taaaaaagac caccgtacga atgccctcgc cgtcattcag tgaataacgc
    11761 tgaatgttga aaatgcgcgc cacatcattg cggcgcgttt ccacaacgtt acagctgatg
    11821 cgctgtccgg cggatgatgt catcctggat ctccttcgac agttcgacaa agaaggcgct
    11881 gtatccggca acgcgcacca ccagcccggc ataatcttgt gggcgctgtt gcgcttcccg
    11941 caacgtgtcg gcgttcacca cgttaaactg aatatgctgc aacttaagct gggtaaacgc
    12001 ccgtaagaag tcggccagtt tgcgtaatcc cgcttcacct tccagggtcg ccggagtgaa
    12061 tttcacgttc agcaacgtac cgttagacag cagcgtatta tcgagcttac tgactgactt
    12121 cagtaccgct gttggccctt gtgcgtcctg gcccagcata ggtgacaagc cgccgtctgc
    12181 cagttgttcc ccggcaaaac gaccgtctgg cgtcgcgcca accaccgatc ccaacgggac
    12241 gtgagcagaa acggtatacg atcccggcgt gaagtaacca ccgcgcgggt tctggtattt
    12301 ttccacttct ttgcagtagt ggcgtaacag ttcggcgctg atgttatcca cctcgtcgat
    12361 atcgttaccg tatttctcga agcggttaat taagcgagca cggacttttt cgccttctgg
    12421 cgttgcaaag ttggctttta ataccgacag caattcgtca aaacttaaac gctgttgctc
    12481 aaaaaccatc cctttcaacg catggagaga atcgctcagg ttggcgatac cgatcccctg
    12541 tacgccggag aagttataac gcgcgccgcc atcggtaata tcgcgacctt tttccagaca
    12601 atcgctgata aaagacgaga gcagcggtac aggtgcccag tcgcgatggc cgatatcaca
    12661 aatattgctg ccctcaacca tcagggtgat gtagtggctg atcttggcgc ggatctgctc
    12721 cagtaaacct tcataagtca gcgcagcatt gccttcattt tcatgcaggc agatttccat
    12781 cactttcagc agattaaaca tcgcgatgtc atgcaagccg taggttctgc cgggaataga
    12841 taattccaca cagcccacta cggaatagtc gcgcgcatct tccagcgaaa cgccacggtt
    12901 gaggaacgct ggtaccacca cttcatcgtt aaagatttgc ggaataccgg tgccgaggcg
    12961 aatggtttcg gcggttttca tcaggaacgg cgtgtcgatc aaggcgttag tgcgcacgcc
    13021 gaggttcggt tgcggtaatt gcacgctttg ataagcgtca aggcaaagga acgaaagcac
    13081 gttcaccgcg ctacgtccgt tctcggttaa cccgccgagc agtgcggtat agccggtcgg
    13141 gaaacctgcg aaataacgcg cgctgctggt ggagcgcaac agcacgatgt cgttgcattt
    13201 cacccataaa gattcgagta gttctttcag gaacgccgga tcttcgccct gggttaaaga
    13261 tgcctgatag aacggcaaca tatactggtc gaagcgcccc aacgatagcg aactggcgtt
    13321 ggattcgtat tgcagaatga tgttcatgta ccagaataac tggcacgcct gccagaacgt
    13381 ctgcggctta tgttcggcgt tatggcggga gatctccgca atagtcagca gttcttcgcg
    13441 acgctgggca tctgtgcagt ttgccgccat tgtttccgcc agttcagcgt aacgcagaat
    13501 gtgtttctgc gaggcttcca gcagtaacag cgcggcctga taaaagtgat tctccggctg
    13561 ttgctgacaa tgttgctgca tctgcgccac cagctccccc agcccgtgat tcagcaggcg
    13621 tgggtaatca ataataatgt gcccctggcc tttatctgtc tggttgatgc tgaaaatctg
    13681 cgtgctggtc gcggctttta cttcatctgt catctgcccg ttgatgaaat ctttcatcga
    13741 acgtttttcc cagtacggga acaactcttc acggtagata cgtttatctt cttcactgat
    13801 agcaaagcgg tcctgcggac gcgtcgggaa ttgatccagc tctttcagca gccagtaagg
    13861 gtccatttcc ggcgacataa tcccagcgcg cggttttacg gtgcggttac cggcaatcag
    13921 ttcttcatca cgaatcgaaa tttcaacatg ttcaaggata tacgctgttg ctttcgcccg
    13981 gcgcaatatc accggttcgc cttcggtttg ccgatggctg gcggtataaa gcagcgcccg
    14041 ctccagcgag atttcacggg tattggcaaa cagtgcagtt ttgaggcgag agatacgatt
    14101 cgtcatgagg aactcctgtg ggaaagcgaa cccgcccttg cgagcgggag aagaacttaa
    14161 gcggtttgcg ccaggtgagc ttcaattttg ctcataatgg cgtcagcacg ttttactgca
    14221 tcgctgatgt tgacgcgcac gatggtcttg ccagcaaaac gctcttcaaa cttgatgccg
    14281 atatctttgg tcagaatcac catatcagcg ctcgccacat cttccgcagt cagttcattt
    14341 tccagaccaa ttgacccctg ggtttctact ttcacttccc agcctttcgc tttcgcggca
    14401 ctttccagcg cctctgctgc catataggta tgggcaacac ctgaaggaca tgcggttact
    14461 gcaataattt tcgtcatggc tcgttcctca attaattaat ttcaaaatcc aaatccaggt
    14521 cgtcttcttt ttcatcagtc gaagacccat ttttacgcgc cagacttttc agcacgttca
    14581 cacaaacagc cgtcaccact gctccgacag ccactgctgc gatataaccc agcttgcctt
    14641 caaccaccgg cagcacaatc agaccacccc agcctgcata gcactgcgca ccgaccagcg
    14701 ccgcagttac tgcaccacaa actgaaccga ccatgatgga aggaatgaca cgcagcggat
    14761 cggcagcggc gaaaggaatc gccccttcag taacgccaac acagcccatc accagtgccg
    14821 ctttgccggt ttcgcgctct tctgcggaga aatttttacg actaatcagc gtcgccagcc
    14881 ccatccccag cggcgggata caaataccca cggcagcgat ggccaccacg gtataaacac
    14941 cctgagcaac gcaaatcagc atgaaggcat aggccacttt gttaaccgga ccgcccatat
    15001 cgaacgccag catcagaccc atgatcaccg ccagcataac aatgctgccc tgctgcatcc
    15061 cctgaagcca ctgagtcagg ctgttggtca acgcccctac aggctcgccc aagccccaca
    15121 tcatgatgcc tgcggtaatc agtgtgccga cgataggaat aatgaagata ggcatcaccg
    15181 agcgcagaac tttatgcacc ggaattttct tcaggtaatg caccacgatg ccgccgataa
    15241 tcccggcgat cagtgcacca aagaagcccg caccaaagct gttaccaacc caggcaccga
    15301 tagcgcacgg agccagcgca gaacgctctg caatggaata accgatgtac gctgcgagga
    15361 aaggcaccat cagcgtcaag cccgcaacgc cgatatcaaa cagttttttc agatttggat
    15421 cggctacggc atccggcact gcgcctttgc catacaacat gacggaaacc gccagcaaaa
    15481 taccgcccga taccacgaag ggaatcatgt gtgaaacgcc cgtcattaaa tgctgacggg
    15541 tatttttcag gatctgcacc aactcattca taagctgctc ccgggcttgc gaaagcccat
    15601 gtccatattt ttgatggagg aaacaatagg caatcgcagg cggagcaaac agtggataaa
    15661 aaggccattt actggacttt tgtaatatca gtacaaaaat gcgatccgcc tcataacttg
    15721 cgataaaccg gaagcatgcc gcaaaacagc gtcttgtgag acgcgtcgca aattgacggt
    15781 tacattacat ttgtccagct tttggcagat ttgtcacatg gcaggccgtt gccgtgcgct
    15841 cttatcctgt aaacagaaaa ccgtaccggg agagaggcaa tggccctgat tgtggaattt
    15901 atttgtgagc tacctaacgg cgtacatgca cgtccggcaa gccacgttga aacgctgtgt
    15961 aatacttttt catcacaaat tgagtggcat aacctgcgca ctgaccgcaa gggcaacgcc
    16021 aaaagcgccc ttgcgctgat tggcaccgat acgctggcgg gcgataactg ccagttactg
    16081 atttccgggg ccgacgaaca ggaagcgcac cagcgtttaa gccaatggct gcgcgatgaa
    16141 ttcccccact gcgatgcgcc gctggcggaa gttaaatctg acgaactgga accactgccg
    16201 gtttcactga ccaatctgaa tccgcaaatt atccgcgccc gcaccgtgtg cagcggtagt
    16261 gcaggcggca ttctgacgcc gatctcttct ttagatctca atgcgctggg taatcttccc
    16321 gcagccaaag gcgttgacgc cgagcaatcc gcactggaaa acggcctgac gctggtactg
    16381 aaaaacattg agtttcgtct gctggatagc gacggtgcca ccagcgcgat tctggaagcg
    16441 caccgatccc tggctggcga tacttccctg cgcgaacatt tactggcagg cgtcagcgcc
    16501 ggattaagct gcgccgaagc gattgtcacc agtgcgaatc acttttgcga agagttcgcc
    16561 cgttccagca gcagctacct gcaagaacgt gccctggacg tacgcgacgt ctgcttccag
    16621 ttactccagc aaatctacgg tgagcaacgc ttcccggcac cgggcaaact gacgcagccc
    16681 gccatttgta tggctgatga actgactccc agccagttcc tcgaactgga taaaaatcac
    16741 ctcaaaggtc tgttactgaa aagtggcggc accacctcac ataccgtaat tcttgcccgt
    16801 tcgttcaaca ttccgacact ggttggtgta gatattgatg cccttactcc gtggcagcat
    16861 caaacgattt atatcgacgg caacgctggg gcgattgtgg ttgagccagg agaagccgta
    16921 gctcgttatt atcagcaaga agcccgcgta caggacgccc tgcgtgagca acagcgtgtc
    16981 tggctgaccc aacaagcccg taccgctgac ggtatccgca ttgaaattgc cgctaacatc
    17041 gctcactccg tggaagcgca ggccgcattc ggcaatggtg cggaaggcgt tggtttgttc
    17101 cgcactgaaa tgctctatat ggatcgcacc agtgcaccgg gcgaaagcga gctgtacaac
    17161 attttttgtc aggcgctgga gtctgccaac ggacgcagca ttattgtgcg cactatggac
    17221 attggcggcg acaaacccgt tgattatctg aacattccgg cagaagctaa cccgttcctc
    17281 ggttatcgcg ctgtgcgtat ttatgaagaa tacgcctcgc tgttcaccac acaactacgg
    17341 tcgatcctgc gcgcctccgc tcacggcagc ctgaaaatca tgatcccgat gatctcctca
    17401 atggaagaga tcttatgggt gaaagaaaaa ctggcggaag ccaaacagca actgcgtaac
    17461 gaacacattc cgtttgatga gaagatccag cttggcatta tgctggaagt accgtcggtg
    17521 atgttcatca tcgatcaatg ctgcgaagag attgatttct ttagtattgg tagtaatgac
    17581 ctgacgcaat atctgctggc agtggatcgc gataacgcta aggttactcg tcactacaac
    17641 agcctgaatc cggcattctt gcgggcgctc gattacgccg tgcaggcggt gcatcgccag
    17701 ggcaaatgga ttggtctgtg cggtgagctg ggagcgaaag gttccgtgct gccgttgctg
    17761 gtcggcttag ggctggatga actcagcatg agcgcaccat caattccggc ggcgaaagcg
    17821 cggatggcgc aacttgatag ccgtgagtgc cgcaagttgc tcaaccaggc aatggcctgc
    17881 cgtacttcgc tggaagtgga acacctgctg gcgcaattcc gcatgaccca acaggacgca
    17941 ccgctggtca ccgccgagtg catcacgctg gaaagtgact ggcgcagcaa agaagaagtg
    18001 ctcaaaggca tgaccgataa cctgctgctg gcgggccgct gccgctatcc gcgtaaactg
    18061 gaagccgact tatgggcgcg cgaggccgtt ttctctaccg gtctgggctt tagttttgcc
    18121 attccacaca gcaaatcaga acacattgag caatccacca tcagcgtggc gcgtctgcaa
    18181 gcgccggtgc gctggggcga tgatgaagcg caattcatca ttatgttaac cctgaacaaa
    18241 cacgctgcgg gcgatcagca catgcgcatt ttctcgcgcc tcgctcgccg catcatgcac
    18301 gaagaattcc gtaacgcgct ggttaacgcc gcctctgccg acgctatcgc cagcctgctg
    18361 caacatgaac tggaactgta aaaggaaaca tcatggaact gtatctggac accgctaacg
    18421 tcgcagaagt cgaacgtctg gcacgcatat tccccattgc cggggtgaca actaacccga
    18481 gcattatcgc tgccagcaag gagtccatat gggaagttct gccgcgtctg caaaaagcga
    18541 ttggtgatga gggcattctg tttgctcaga ctatgagtcg tgacgcgcag ggtatggtgg
    18601 aagaagcgaa gcgcctgcgc gacgccattc cgggcattgt ggtgaaaatc ccggtgactt
    18661 ccgaaggtct ggccgccatt aagatgctga aaaaagaggg tattactacg cttggcaccg
    18721 cagtgtacag tgctgcacaa gggttactgg ccgcgctggc aggggcaaaa tacgttgctc
    18781 cgtatgttaa ccgcgtagat gcccagggcg gggacggcat tcgtacggtt caggagctgc
    18841 aagcgctgtt agaaatgcac gcaccagaaa gcatggtgct ggcggccagc tttaaaacgc
    18901 cgcgtcaggc gctggactgt ttactggcag gatgtgaatc catcaccctg cccttagatg
    18961 tagcgcaaca aatgctcaac acccctgcgg tagagtcagc tatagagaag ttcgaacacg
    19021 actggaatgc cgcatttggc actactcatc tctaaaggag caattatgga ccgcattatt
    19081 caatcaccgg gtaaatacat ccagggcgct gatgtgatta atcgtctggg cgaatacctg
    19141 aagccgctgg cagaacgctg gttagtggtg ggtgacaaat ttgttttagg ttttgctcaa
    19201 tccactgtcg agaaaagctt taaagatgct ggactggtag tagaaattgc gccgtttggc
    19261 ggtgaatgtt cgcaaaatga gatcgaccgt ctgcgtggca tcgcggagac tgcgcagtgt
    19321 ggcgcaattc tcggtatcgg tggcggaaaa accctcgata ctgccaaagc actggcacat
    19381 ttcatgggtg ttccggtagc gatcgcaccg actatcgcct ctaccgatgc accgtgcagc
    19441 gcattgtctg ttatctacac cgatgagggt gagtttgacc gctatctgct gttgccaaat
    19501 aacccgaata tggtcattgt cgacaccaaa atcgtcgcgg gcgcacctgc acgtctgtta
    19561 gcagcgggta tcggcgatgc gctggcaacc tggtttgaag cgcgtgcttg ctctcgtagc
    19621 ggcgcgacca ccatggcggg cggcaagtgc acccaggcgg cgctggcact ggctgaactg
    19681 tgctacaaca ccctgctgga agaaggcgaa aaagcgatgc ttgccgccga acagcatgta
    19741 gtaaccccgg cgctggagcg cgtgattgaa gcgaacacct atttgagcgg cgttggtttt
    19801 gaaagcggcg ggctggcggc ggctcacgcg gtgcataacg gcctgaccgc tatcccggac
    19861 gcgcatcact attatcacgg tgaaaaagtg gcatttggta cgctgacgca gctggttctg
    19921 gaaaacgcac cggttgagga aatcgaaacc gtagctgcac ttagccatgc ggtaggtttg
    19981 ccaataactc tcgctcaact ggatattaaa gaagatgtcc cggcgaaaat gcgaattgtg
    20041 gcagaagcgg catgtgcaga aggtgaaacc attcacaaca tgcctggcgg cgcgacgcca
    20101 gatcaggttt acgccgctct gctggtagcc gaccagtacg gtcagcgttt cctgcaagag
    20161 tgggaataac ctactccaaa ctcccggctt gtccgggagt ttgaacgcaa aattgcctga
    20221 tgcgctacgt ttatcaagcc tacgaaatct ctgcaatata ttgaatatgc gtgcttttgt
    20281 aggccggata aggcgttcac tccgcatccg gcactctgtg ccagtaatct gaaactccca
    20341 cgttgttcag gagtttttta tccgcttctg gacattttct ccacagaaca atcgttagcc
    20401 cggaaggtcg aatctgtgac tcttattttc acggacgaac cagatgaaag cttcactggc
    20461 gctgctcagc ctgctaacgg catttaccag ccactcctta aaatctcccg ccgttccacc
    20521 aaccgtggta caaatccagg ccaataccaa ccttgccatc gcagacggtg ccagacagca
    20581 aatcggcagc acgctatttt acgatccggc gtatatgcag cttacctatc ctggcggtga
    20641 tgtcccgcaa gaacgcgggg tatgttccga tgtggtgatc cgcgccctgc gcagccagaa
    20701 ggtcgatttg cagaaactgg ttcacgaaga tatggcgaag aattttgccg agtacccgca
    20761 aaaatggaag ttaaagcgcc cggacagcaa catcgatcac cgtcgggtgc ctaatctgga
    20821 aacctggttt agccgccaca acaaaacacg ccccatcagt aaaaatgccg gtgactatca
    20881 agcgggcgat attgtctcct ggcgattaga taacgggctg gcgcatattg gggtggtgtc
    20941 agatggcttc gcccgcgacg gtacgccgct ggtgatacat aacattggcg caggcgcgca
    21001 ggaagaagat gtactgttta gctggcggat ggtggggcat tatcggtatt ttgttaaata
    21061 agcgatcgcc ccacatgaag aaaaaattca gatgcttttt acactgacag cttctttttt
    21121 cttacggctc accagcgcca gtgccagcac aatcagcaca ataccgctac cttcaaccac
    21181 gccaggattc tcgccgagca gccaccagga aaagagtacg ccgcaaacgg gaacggccag
    21241 tgtgcttaag ctggcaatac tggcaggcaa gtttttcaat acaaacaacc ataagctcca
    21301 cgccagcgcc gtcgccagaa tcgcactgta gaccagcgcc cagaacacag tgggctgcca
    21361 gtcaatttca cgttgtggca ccagtaaagc gaccacactc atcaccagcg ccgcatacag
    21421 catctgccag gatgttaacg acaataaatc cacgcgcgga tgacgggcat acagacgttt
    21481 agcaacaatc gcgctcgccc cccaactaac accggagaga attgccagca tggcactttt
    21541 catcgaagag aaatcgagtt gccacggctg caacaccaaa aataagccga aagcggcaat
    21601 cagtatcgcg aaatattgcc cacgtcgcag gcgttcaccg agaaacaacg cggcgaaaat
    21661 caccacccag aacggcatgg tatagctcag gatcgccacc ttccccgcac ctccgctgac
    21721 caacgcccat tgtgccaggc caatcattcc gcaggtttgt aacagggcaa tagctaaggt
    21781 gtatttaaac ggcgtcgggc gcattgcgcg accacgtaat aaaaggacga tgaataaaac
    21841 gagagcgccg aaaatgcagc gtaaggcggt gaagtcgaag gcaccgatgt aactggtgac
    21901 ttgcttcatg aaaatccagc tataactcca gataagtgtg agcacaacca ggccactgat
    21961 tgccagtggg ttgctctttc ctgcggcaga catgagagat ccagtgtgta gttttcctgt
    22021 ggacactata cgccggtaaa gaaacttcag cgactgcctg aaagacagtc gctgaagaag
    22081 gtcagattac agcaggtcga aacggtcgag gttcatcact ttcacccatg ccgccacgaa
    22141 gtctttaaca aacttctcgt gggcatcgct gctggcgtaa acttccgcta ccgcacgcag
    22201 gacagagtta gaaccaaata ccagatccgc acggctggct gtgtatttca cttcgccggt
    22261 ttcacggtca cgaccttcga acagctcttt cgattcgtca gtcgctttcc actcgtaacg
    22321 catatccagc aagttcacga agaagtcatt gctcaatacg ccaacgcggt cagtgaagac
    22381 gccgtttttg ctgccatcga agttggcacc cagtacacgc atcccgccca ccagcgcagt
    22441 catttccggc gcggtcagcg tcagttgctg tgctttgtcg atcagcagtg actcggtggt
    22501 ggaaacgtcc agacgagcgc gatagttacg gaaaccgtca gcaattggct ccagcagctc
    22561 aaacatctca atgtcagtct gatcctgacg cgcatcaacg cgccccggcg caaacggtac
    22621 atgaatgctc aaacctgcgg cgcttgcggc tttctcaaca ccaaccacac cagccagcac
    22681 gatgatatcc gccagcgagg ctttaccaga ctctttctgg attttctcca gaacaggcag
    22741 agcacgaacg gctgcggcgt tcacatccca gtcgcgctgc ggcattaatg ccagacgcgc
    22801 accgttagca ccaccgcgtt tgtcgccacc acggaaggta gaagcagatg cccaggccac
    22861 cgataccagc tcactaacag acagaccaga atccgcaatc gcgaatttca gatcgataat
    22921 gtcctgctcg gtcgggttgt agatcggctg cggcagcgga tcttgccaga tcagatcttc
    22981 tttcggtact tccggtccga tgtaacgaga tttcggcccc atatccctgt gcgtcagttt
    23041 gaaccaggca cgggcaaagg cttcgttgaa cgcctgcgga tcgttgagga aacgacgaga
    23101 gatcttctcg aactcaggat caaaacgcag cgtcaggtcg gtcaccagca tcgtcggttt
    23161 acgtttcttc gacggatcga acggatccgg gataatttcc ggtgcgtcta ccgcttcgaa
    23221 ctggattgcg ccagccggac tgcgggtctg tacccactca tatttgaaca ggttctcgaa
    23281 gaaatagttg ctccactggg tcggcgtctg ggtccagaca acttccagac cagaggtaat
    23341 ggcatctgcg ccaacgccgc tgccgtaagt gctcgcccag cctaaacctt gttcttcaat
    23401 cggtgcagct tctggatcag gacctacatt tgatgtcgga ccggcaccgt gggttttacc
    23461 cagcgtatga ccacccgcaa tcagcgccac ggtttcttcg tcgttcatgc ccatgttgcc
    23521 gaaggtcgcg cggatagctg ctgccgcaga aagcggttcg ccgctgtggt ccgggccttc
    23581 cgggttaacg taaatcaggc ccatctcggt tgcacccagc ggtgctttcg ccagcgcttc
    23641 cggatgacgg tgagtcagcc aggctttttc atcaccccag ttaacatcca gatccggttc
    23701 ccagacgtct tcacgaccgg caccaaaacc gaaggtacgg aagccggagt tttccagcgc
    23761 cacgttacct gcgaggataa acaggtcggc ccaggagatt ttctgaccat atttctgttt
    23821 gattggccac aacaggcgac gcgctttatc gaggcttacg ttatccggcc aggagttcag
    23881 cggtgcaaaa cgttgctgac cacgacccgc gccgccgcgt ccatcgattg aacggtaagt
    23941 acccgcgccg tgccaggcca tacgaataaa cagaccggcg taactgcccc agtcggcagg
    24001 ccaccacggc tgagattctg tcagcagggc tttcagatct tttttcaggc cgtagtaatc
    24061 taatttgctg aattctttgc ggtagtcaaa gtcctcaccc agtgggttag aacgattaga
    24121 atgttggttt aacaggtcaa cacggagttg atttggccac cagtcgcgag tggttgtgcc
    24181 cgcccccgcg ctctggtcgt gaccgccctg atggaacggg catttgccag tggctgtggt
    24241 gttatgaata tcgtctgacg tgctcatcaa tgtgctcccc tctacagtgt taccgttacg
    24301 atacacagcg ttagagagaa gttataattg aattaatcca cggatgcgat agttgagatc
    24361 ttacatattg ttggttaaag agatgtagat caaattgatc gtaattaagc ttttttgatt
    24421 atgtccgaat tcggacaaat gctttataaa aaagggtttt aggttcatat ccttcaagta
    24481 atgagggaga aaacaaggct gataacctta ttttcccgcc ctcatttcga ggcagcattt
    24541 tgtgctctgt ttaaaatttg tgatcactgt gtgattttca caaaagccac actatttata
    24601 aaccaggtcg aacacccagc gtatggcaaa tcgcgtaact catttcagcg cggttaagcg
    24661 tatagaagtg gaaatctttc actccttcac ggcttaaaat cttcaccata tccatggcaa
    24721 tattcgctcc caccagtttg cgggtttcag catcgtcatc cagcccgtca aacatttgtg
    24781 ccatccacgc cggaatacgc acgttggtca tatcggcaaa tttcttcgcc tgtttaaagt
    24841 tagataccgg caaaatcccc ggaataattt ccacatcaat gcccgccgat acgcagcggt
    24901 cacgaaaacg caggtagctt tcgacatcaa agaagaactg agtaatcgcg cggttggctc
    24961 cggcatcgac tttgcgtttc aggttgagca aatccgcctg agcgcttttt gcttccgggt
    25021 gaacttccgg atacgccgcc acggagatat cgaaatctgc cacttctttt aacagcgtca
    25081 ccaggtcaga agcatacatt tccggcttac cacttcccgg cggcagatcg ccacgcagcg
    25141 ccacgatatg acgaataccg ttattccagt agtcgcgtgc aatggtgcgc agctcgtcgg
    25201 gcgtcgcatc aatgcaagta agatgcggtg ccgcttccag accagtgcga tctttaatgc
    25261 ctttaataat gctgtgcgta cggtcgcgct cgccggagtt cgcgccatag gtcaccgata
    25321 caaacttcgg tttcaggctg ctaaggcgat cgatggagtt ccacagggtc tgctccattt
    25381 cactggtacg cggcgggaaa aactcgaacg aaacgttaat ctgcccctgg acttctgcca
    25441 ggctctgatt cagggcatcc cgctggctgg cgtgaaaaaa gctcatacct tacctcatca
    25501 atcgcttgta gttatatgtt gtgttgtgca catctatcca tttagacgtc cagatgtaaa
    25561 gatgaaggaa aagccgaagg gcgtcaaccg aaaaatgacc aatgatggtg aggaaaaatc
    25621 agggaagatg aaaaaacttc aggatggtaa gaaaaagaaa atccggtatg caaagcaaac
    25681 cggactgcaa atataaacta accatgtact tattgcttct tgccggattc gtcgtaaacg
    25741 cttcatccgg cctaatccta tgataattca atatatttca ttagaaacca tagacccgaa
    25801 aacatgagta ccgggcatta ttaaatttct gaaattacaa caactgtgcc agccggttga
    25861 tatccgactg aatcgccccg gcggtgacgt cgcgcccagc gccaggccca cggatcacca
    25921 gcggattatc gcggtaccag cggctttcga tggcaaagac gttatcgcat ggcagcagtg
    25981 atgccagcgg atggtcttca cgcaccgctt ccacgcccac acgcgctttg ccattggcat
    26041 cgaaacgcgc cacgtagcgc agcaccagcc ccatttcgcg ggccgcttcc agccgttgca
    26101 ccatctgctc gttaagttca tcgccatttt caaagaaatg gtcgatgctg ccgccttcgc
    26161 aatgagcagg caccagcgat tccacacgta cctggtccgg ttcaatgttg taacccgctt
    26221 cacgcgccag aattaccagc ttgcgcatta cgtctttgcc ggagaggtcg tcacgcggat
    26281 caggctcggt taagccctgc tgccacgcct gatccaccag ctcggtaaac ggcacgctac
    26341 cgtcgaattg caggaacagc caggagagcg tgccggagaa gatcccgctg atcgacaaaa
    26401 tagtatcgcc gctgtcgatc agatcgcgca cggtgtggtt gatcggcaag cccgcaccga
    26461 cggtggcatt gtacagccag tgacgcccgg ttttttcgaa ggcgtcgtgg atctggcgat
    26521 atttattgct gtcgctggct cccgccagtt tgttggcgct gataacgtgg aaaccgtggc
    26581 tggcgaaatc aagatactga tcagcaagct gctggctggc ggtaacgtcc agcaccacta
    26641 aatcatcata cggatgggcg cgcatccaca ggaacaacga ctcttcatcc tgctcaaccg
    26701 cttcatcgtt gaagaaggct aacgcgcggc tggcgtccag cccgtcatag ctcaacaggc
    26761 tgcggcggct gtccaccaca cctgccagca caaactcaaa gccggtacgt gccgaaagcg
    26821 tgctctgctc acgggcgaac agttccagcc aacgggaacc gatattgccc ttaccgaaca
    26881 ataccaggcc gatgcgtttt tctgcgcgga agacggactg atgcagcccc tgaatcaggc
    26941 tttcggtcgg gccggtgcgc agtactgcca ccaggctgat gccgtcatcg gactgccagg
    27001 taaattcgac cggctggcct ttcagttgct gccagaagcg gtggcaatgc agcgggttac
    27061 gggtgacgcc tgcaccgacc atcgccacca gcgccagccc ctgacgcagg cgcagttcgc
    27121 caggtaatcc cgcttcgtcg aggattttca gcgcactgtc ggccacttct gaggtgtagc
    27181 aaaattgcag caactggcga tcgttatgta cgccaaccgc cagcgggcgt acctgcgcgc
    27241 gtttcaggat ttggtcgatc tctttatgcg ccagtttgaa atcctgactg gcgggcacct
    27301 gaaactcaat caaacagaca tcatcgtggc tggtgacaat acgcgcacca gtaccggagg
    27361 ccagcacgcg ttcaatgcgc gtggaacctt gatccggcgt gtagctacag cgcagttgca
    27421 ggtcgatttc gctgccagaa accggctgta aagtacgggc gtgaagaacg ggagccgcca
    27481 ggcgcgccag ttcgctggcc tcatccagac gcagcaacgg cagcaggcag gcatctttca
    27541 ctttacgcgg gtcggcactg tataccccgg cgacgtcgct ccagatggtt acgcgagaaa
    27601 cacccgccag cgcaccgatt tgtgtcgcgg aatagtcgga accgttacgc cccagcagca
    27661 ccgtttcacc ggcgttgttg cggctgataa atccggtcac caccagacgt ttgcccggat
    27721 gttgcaccag cagctgttgc agcaacgggt aagaaagccc ttcatcaacc tgcggttgtg
    27781 cggcgcgttc agcgcgtaaa aactcgcggg catcaagcca ggccgctggc agcccttgtt
    27841 gattaagtac cgcagacatc aggcgtgccg accacacttc accgtgcccc accacttccg
    27901 catacactgc gtcgttaata ccgctgtcga gcagcgccgc caggcgctca aggtcgctga
    27961 caaaagcgct aatgaggcta tcggcttctt cagcgggtag cagaccgcta atcagatcgc
    28021 actgatagcg acgcagcgtt tgttgaacct gatgcgcaga gagacgatcg gtctggctta
    28081 gtttcaacca gttaatcaac tggttagtgg tgctaccggc ggcggaaacc accatcatat
    28141 cgtcaggctg agagtactcc gccataatgc ccgcgacacg caaataacac ttcacatcag
    28201 ccagactact gccaccaaat ttatgcagct gacgaccttt cgcccctgcc tgcgcaatca
    28261 cactcatttt taccccttgt ttgcagcccg gaagccattt tccaggtcgg caattaaatc
    28321 ttcgccatct tcaataccgg tggagatacg cagcagcgtc tcggagatcc cggcggcagc
    28381 acgcgcttct ggtgccatgc ctgcatgtgt catggttgcg gcgtgagaga ttaaactttc
    28441 cactccccct aatgattccg ccagcgtaaa caacgacagc ccgctcagga aacgacgcag
    28501 cgtctgctca tcgccatcca gttcaaaact caacattgcg ccaaagcctt tttgctgacg
    28561 cgcggcaatt tcatgcccct gattttccgg cagcgacggg tgatacagtt ttttcaccaa
    28621 cggctgggtt tgcagatatt tcacaatcgc ctgcgcgtta cgctgggcca actccatgcg
    28681 cggcaccagc gttcgcaacc cgcgtagtag cagatagctg tcaaacgcgc cgcccgtcac
    28741 gccaatattg tttgcccacc aggcaagttc agtgacaacg tccgggtctt tagcaatcac
    28801 cacgccggcc accacgtctg agtgaccgtt cagatatttc gtgcatgaat gcaacaccag
    28861 atcggcacct aatgccagcg gattttgtaa tgccgggctt aagaaggtgt tatccaccac
    28921 gctcaccgcc ccgacttccc ttgccagatg gcagattttc gcaatatcca cgacgcgtaa
    28981 caatggatta cttgggcttt ctaccagtac cagtttgggt ttttctgcca gcgctgcccg
    29041 taatgcctgt tcatcgcctt gatcaacaaa caacacgcga tagcaaccac gtttcgccag
    29101 gctgtcgaac aggcgatagc taccgccgta gcagtcgtgc ggcgctacca gcagatcgcc
    29161 aggtttcaaa aagacggtcg ttaccaggtg aatcgcggac atgccggtat tagtaagtac
    29221 tgcaccagca ccgccttcca gttctgccag tgctcgctga accacatcgc gcgttgggtt
    29281 gccacgacgc gagtaatcat gcgcgcgcgg ttcattaaat ccggtaaagt tataggtact
    29341 ggaaagatgg atcggtggga caacgcaacc atactgttcg tcgtcattta acccgctacg
    29401 cactgcgatg gtggcctgtt tacgcgtcat gtgatgaagt tccctgggct tggtcggtga
    29461 aatgtcaggc accagagtaa acattgtgtt aatggacgtc aatacatcta gacatctaaa
    29521 cttctttgcg tatagattga gcaaatccca aatagccgtt aaaattatat gcattatcac
    29581 gccgacaggt gcattacacg atgtcacggt aacgcctgta cggtaaacta tgcgggttta
    29641 cggtcagtac ccacataaat tgtgtggtct ggtctcaatt tattgacgaa gaggattaag
    29701 tatctcatgg ctgaatggag cggcgaatat atcagcccat acgctgagca cggcaagaag
    29761 agtgaacaag tcaaaaagat tacggtttcc attcctctta aggtgttaaa aatcctcacc
    29821 gatgaacgca cgcgtcgtca ggtgaacaac ctgcgtcacg ctaccaacag cgagctgctg
    29881 tgcgaagcgt ttctgcatgc ctttaccggg caacctctgc cggatgatgc cgatctgcgt
    29941 aaagagcgca gcgacgaaat cccggaagcg gcaaaagaga tcatgcgtga gatggggatt
    30001 aacccggaga cgtgggaata ctaaagcaaa aaagagcggc gcggagtgga atcgcctgat
    30061 gcgctacgct tatcaggcct acgtcatatt gcaatttatt gaatttgcac gaacttgtag
    30121 gccggataag gcgttcacgc cgcatccggc ataaacaacg agcacgttgt ctgcgaccca
    30181 ccgcttttta tacatggacg tttaactatg aaaaacaggc tgctgatcct cagcctgctg
    30241 gtttctgtac ctgcctttgc ctggcagccg caaaccggcg acatcatctt tcagatctct
    30301 cgctcatcgc aaagtaaagc gatccaactg gcgacccatt ccgattacag ccacaccggt
    30361 atgctggtga tacgcaacaa aaagccctac gtttttgaag cggtcggccc ggtaaaatac
    30421 accccgctca agcagtggat cgcccatggt gaaaagggta agtacgttgt tcgccgcgtt
    30481 gaaggcggac tgagtgttga acaacagcaa aaactggcgc aaacggcaaa acgttatctc
    30541 ggtaaaccgt atgatttcag cttctcatgg agcgacgatc gccagtactg ttcggaagtg
    30601 gtgtggaagg tttaccagaa cgcgctggga atgcgcgtgg gcgagcagca gaagttgaaa
    30661 gagttcgatc tcagcaaccc gctggtccag gcgaagctca aagaacgcta cggcaaaaat
    30721 attccgcttg aggaaacggt agtttcacca caagccgttt tcgacgcgcc acaactcacc
    30781 acggttgcca aagagtggcc gctgttttcg tggtaatgga tgatgaaaaa aataattccc
    30841 gccgtagtct gtttgttgct ttgtagttgc acgacctttc cgcaagctgt cgctccggta
    30901 aacggtgatg cctccaatta gttgaattaa ggatataata ctcgctttta aggttgtttc
    30961 atggtcacag ttaaccttca ttgcccccgt tgccagtccg ttcaggttta ccgtcacggg
    31021 cagaacccaa aaagacatga tcgattccgc ggccgtgact gtcaccgggt atttcagctc
    31081 agttactgtt acgagccccg taaaccggga gttaaagacc aaatcgctga aatgactttc
    31141 aacggtgccg gggttcgcga tactgcccgt acgctgaaaa tcggcattaa tacagtcatc
    31201 cgaactttaa aaaactctcg ccacgaagaa taacgtcatc cccggtcgct catgctgatg
    31261 tggcactcat ctgcaaactt agtcagcaat ggagctttgt cggcagtaaa gcgcgacagc
    31321 actggctctg gtacgtgtac aacacgaaaa ctggtggtgt tctggcctac acttttggcc
    31381 cccgggctga cgaaacctgt cgtgaattgc gggcgctgct taccctttta ccatcggcat
    31441 gttgaccact gacgaatggg gtagttacgc tagagagtta ccgaaggaga agcatctgac
    31501 tggtaagatt tttactcagc acattgagcg taacaatctg accctgagaa cccgaatcaa
    31561 acgtctggcc cgcagaacca tctgtttctc gcgttctgta gaacttcatg aaaaagtcat
    31621 cggtgctttt atcgaaaata tatgttctac taattggagt catcaccctt ctgataagct
    31681 ttccttggat gattaccaga ctgaatacac agccaaagat ttttacctca ttctgctctt
    31741 ttttctcaaa tgacatatat tatattttac atcatattca taggcatatg aca
//
LOCUS       DBANUU010000048        31544 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000048 DBANUU010000000
VERSION     DBANUU010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.048"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..860)
                     /gene="rarD"
                     /locus_tag="ACIE68_002099"
     CDS             complement(<1..860)
                     /gene="rarD"
                     /locus_tag="ACIE68_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFX5908410.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDA"
     gene            complement(912..1379)
                     /gene="yigI"
                     /locus_tag="ACIE68_002100"
     CDS             complement(912..1379)
                     /gene="yigI"
                     /locus_tag="ACIE68_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFX5908411.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1544..2413
                     /gene="pldA"
                     /locus_tag="ACIE68_002101"
     CDS             1544..2413
                     /gene="pldA"
                     /locus_tag="ACIE68_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFX5908412.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNSFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2546..4375
                     /gene="recQ"
                     /locus_tag="ACIE68_002102"
     CDS             2546..4375
                     /gene="recQ"
                     /locus_tag="ACIE68_002102"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFX5908413.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4439..5059
                     /gene="rhtC"
                     /locus_tag="ACIE68_002103"
     CDS             4439..5059
                     /gene="rhtC"
                     /locus_tag="ACIE68_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFX5908414.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5121..5741)
                     /gene="rhtB"
                     /locus_tag="ACIE68_002104"
     CDS             complement(5121..5741)
                     /gene="rhtB"
                     /locus_tag="ACIE68_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFX5908415.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5852..6874
                     /gene="pldB"
                     /locus_tag="ACIE68_002105"
     CDS             5852..6874
                     /gene="pldB"
                     /locus_tag="ACIE68_002105"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFX5908416.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6882..7682
                     /gene="yigL"
                     /locus_tag="ACIE68_002106"
     CDS             6882..7682
                     /gene="yigL"
                     /locus_tag="ACIE68_002106"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFX5908417.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTAHGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGSNADDAVPHYLRKLYLS"
     gene            7758..8657
                     /gene="bioP"
                     /locus_tag="ACIE68_002107"
     CDS             7758..8657
                     /gene="bioP"
                     /locus_tag="ACIE68_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFX5908418.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8545..9498)
                     /gene="metR"
                     /locus_tag="ACIE68_002108"
     CDS             complement(8545..9498)
                     /gene="metR"
                     /locus_tag="ACIE68_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFX5908419.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9616..11877
                     /gene="metE"
                     /locus_tag="ACIE68_002109"
     CDS             9616..11877
                     /gene="metE"
                     /locus_tag="ACIE68_002109"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFX5908420.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWTETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(11916..12731)
                     /locus_tag="ACIE68_002110"
     CDS             complement(11916..12731)
                     /locus_tag="ACIE68_002110"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFX5908421.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            12993..13754
                     /gene="udp"
                     /locus_tag="ACIE68_002111"
     CDS             12993..13754
                     /gene="udp"
                     /locus_tag="ACIE68_002111"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFX5908422.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            13895..15322
                     /gene="rmuC"
                     /locus_tag="ACIE68_002112"
     CDS             13895..15322
                     /gene="rmuC"
                     /locus_tag="ACIE68_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFX5908423.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQYAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15417..16172
                     /gene="ubiE"
                     /locus_tag="ACIE68_002113"
     CDS             15417..16172
                     /gene="ubiE"
                     /locus_tag="ACIE68_002113"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFX5908424.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16186..16791
                     /gene="ubiJ"
                     /locus_tag="ACIE68_002114"
     CDS             16186..16791
                     /gene="ubiJ"
                     /locus_tag="ACIE68_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFX5908425.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            16788..18428
                     /gene="ubiB"
                     /locus_tag="ACIE68_002115"
     CDS             16788..18428
                     /gene="ubiB"
                     /locus_tag="ACIE68_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFX5908426.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELAYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18507..18776
                     /gene="tatA"
                     /locus_tag="ACIE68_002116"
     CDS             18507..18776
                     /gene="tatA"
                     /locus_tag="ACIE68_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFX5908427.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            18780..19295
                     /gene="tatB"
                     /locus_tag="ACIE68_002117"
     CDS             18780..19295
                     /gene="tatB"
                     /locus_tag="ACIE68_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFX5908428.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19298..20074
                     /gene="tatC"
                     /locus_tag="ACIE68_002118"
     CDS             19298..20074
                     /gene="tatC"
                     /locus_tag="ACIE68_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFX5908429.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20116..20898
                     /gene="tatD"
                     /locus_tag="ACIE68_002119"
     CDS             20116..20898
                     /gene="tatD"
                     /locus_tag="ACIE68_002119"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFX5908430.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(20895..21383)
                     /gene="rfaH"
                     /locus_tag="ACIE68_002120"
     CDS             complement(20895..21383)
                     /gene="rfaH"
                     /locus_tag="ACIE68_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFX5908431.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21550..23043
                     /gene="ubiD"
                     /locus_tag="ACIE68_002121"
     CDS             21550..23043
                     /gene="ubiD"
                     /locus_tag="ACIE68_002121"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFX5908432.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23089..23790
                     /gene="fre"
                     /locus_tag="ACIE68_002122"
     CDS             23089..23790
                     /gene="fre"
                     /locus_tag="ACIE68_002122"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFX5908433.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(23974..25137)
                     /gene="fadA"
                     /locus_tag="ACIE68_002123"
     CDS             complement(23974..25137)
                     /gene="fadA"
                     /locus_tag="ACIE68_002123"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFX5908434.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25147..27336)
                     /gene="fadB"
                     /locus_tag="ACIE68_002124"
     CDS             complement(25147..27336)
                     /gene="fadB"
                     /locus_tag="ACIE68_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFX5908435.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLQFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27526..28857
                     /gene="pepQ"
                     /locus_tag="ACIE68_002125"
     CDS             27526..28857
                     /gene="pepQ"
                     /locus_tag="ACIE68_002125"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFX5908436.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            28857..29471
                     /locus_tag="ACIE68_002126"
     CDS             28857..29471
                     /locus_tag="ACIE68_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFX5908437.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29510..30961
                     /gene="trkH"
                     /locus_tag="ACIE68_002127"
     CDS             29510..30961
                     /gene="trkH"
                     /locus_tag="ACIE68_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFX5908438.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            30973..31518
                     /gene="hemG"
                     /locus_tag="ACIE68_002128"
     CDS             30973..31518
                     /gene="hemG"
                     /locus_tag="ACIE68_002128"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFX5908439.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7295 a         8060 c         8661 g         7528 t
ORIGIN      
        1 gcatccatca caaaaattgc cagcgccacc caaataaagg cgaaagtcac catcttatcg
       61 gcacccggtt tttcaccata aaacgtcaca gccagcagga acatcagcgt cgggccaatg
      121 tactggaaaa agcctaacgt tgagagacgc aggcgcgtgg cagcggcagt aaaacacaac
      181 agcggtacgg tagtgacaat accggcggcg atcagcagta aattcagcga catcgggttt
      241 tgccccatat ggctggttga gctgtcggca atagcaaaca ggtaaatcgc cgccacgggc
      301 agcagccaca tggtttcaat taacatgccg gtttgtgctt ctaccgcaat cttcttgcgc
      361 accaggccgt agaaggcaaa actaaatgcc agccccagcg cgataatagg tagcgaacca
      421 aaagtccaca gctggactaa cacgccacat atcgccagaa tcaccgccag ccattgcatc
      481 cggcggaatc gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata
      541 aagtaaccaa ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc
      601 cagttgccac caatcagcac ggcagagact gccagcataa aaattttctg tggcgtctga
      661 atcagcgttt ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa
      721 aacgaccaga tcacgcgatg cgtcaggatt tcatcggcgg gcacgtagta aatcaacttg
      781 aagtacgctg gcgctatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc
      841 cgcgtttgtt ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg
      901 cttttatgtc ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct
      961 tcattgtgta attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg
     1021 gtagcagtaa aacgctcgcc cctgcctggg cgcagataat caacgcgaag atcaatggtc
     1081 cccatccgcg atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag
     1141 gtacttccca cgcacaccag accggcggcg acatccagcg ccgacgcaat gactccgccg
     1201 tgcaaaatgc tttgcgccca gttacccacc atcatcggct gatttttaaa ggccagctgt
     1261 gcgaactctt tttcgtaacg ctccagctcc atccctaacg cgcggttaaa tggcatgtga
     1321 taaacaaaca tctcacccac taatttcagg gcttgttcag cggtcagtac ggcagacata
     1381 tcatccttat acttcattgg ttaatgaaat gttgatttta tgcttctttg ttgttggttt
     1441 ctactttagg aagggataac taacggcaat ggagttaagt atgtaaaata gcccgcagaa
     1501 aaacattcac cttatcaata attcgttacg gagaacacga ccgatgcgga ctctgcaggg
     1561 ctggttgttg ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa
     1621 agaggtgcat gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca
     1681 tgacaattcg ttcacgctct atccttatga caccaactac ctcatctaca cccaaaccag
     1741 cgatctgaac aaagaagcga ttgccagtta cgactgggcg gaaaatgcgc gtaaggatga
     1801 agtaaagttt cagttgagcc tggcatttcc actgtggcgt gggattttag gcccgaactc
     1861 ggtgttgggt gcgtcttata cgcaaaaatc ctggtggcaa ctgtccaata gcgaagagtc
     1921 ttcaccgttt cgtgaaacca actacgaacc gcaattgttc ctcggttttg ccaccgatta
     1981 ccgttttgca ggttggacgc tgcgcgatgt ggagatgggg tataaccacg actctaacgg
     2041 gcgttcagac ccgacctccc gcagctggaa ccgcctttat actcgcctga tggcagaaaa
     2101 cggtaactgg ctggtagaag tgaagccgtg gtatgtggtg ggtaatactg acgataaccc
     2161 ggatatcacc aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc
     2221 ggtgctcagt gcgaaaggac agtacaactg gaacaccggc tacggcggcg cggagttagg
     2281 cttaagttac ccgatcacca aacatgtgcg cctttatact caggtttaca gcggctatgg
     2341 cgaatcgctc atcgactata acttcaacca gacccgtgtc ggtgtggggg ttatgctaaa
     2401 cgatttgttt tgatgaacgg ttgagtggtt ggcaaatctg gaatccagca tccaggatta
     2461 ccctctcaga gactaaaagc attgcagttt ctcgcgcagg cgctgaaaat agcgcctgtt
     2521 tttatttcag tcaatcgggg tgaatgtggc gcaggcggaa gtgttgaatc tggagtccgg
     2581 agctaaacag gttttacaag aaacctttgg ctaccaacag tttcgccccg gccaggaaga
     2641 aattatcgac actgtgcttt ccggtcgcga ttgcctggtc gtcatgccca ccggtggcgg
     2701 aaaatccctt tgctatcaaa ttcctgcctt attgctaaac ggccttaccg tggttgtttc
     2761 accgctgatt tcgttgatga aagatcaggt ggatcaactg caagccaacg gcgtggcggc
     2821 ggcgtgcctt aactcgacgc aaacccgcga gcagcaactt gaagtgatga caggctgtcg
     2881 caccgggcaa atccgcctgc tttatatcgc gccggaacgc ctgatgctgg ataactttct
     2941 tgagcatctg gcgcactgga atccggtgtt attagccgtt gatgaagcgc actgtatctc
     3001 ccaatggggc cacgatttcc gcccggaata tgccgcgctc ggtcagttgc gccagcggtt
     3061 cccgacgctg ccgtttatgg cgctgaccgc cacagccgac gacaccacgc gccaggatat
     3121 cgtgcgcctg ctggggctga acgatccgct gattcaaatc agcagttttg accgtccgaa
     3181 tattcgctac atgctgatgg agaaattcaa accgctcgat cagttgatgc gctacgtgca
     3241 ggaacagcgc ggtaagtccg gcattatcta ctgcaacagc cgggcgaaag tagaagacac
     3301 cgctgcgcgc ctgcaaagca agggtattag cgcggcggcc tatcatgccg ggctggaaaa
     3361 taatgtccgc gccgacgtgc aggagaaatt ccagcgcgat gacctgcaaa ttgtggtggc
     3421 gacggtggcg ttcggcatgg gcatcaataa accaaacgtt cgcttcgtgg tccactttga
     3481 tattccgcgc aatatcgaat cctattatca ggaaaccggt cgcgccgggc gtgatggcct
     3541 gcctgcggaa gcgatgctgt tttacgatcc ggctgatatg gcgtggctgc gccgttgtct
     3601 ggaagagaag ccgcaggggc agttgcagga tatcgagcgc cacaaactca atgcgatggg
     3661 cgcgtttgcc gaagcgcaaa cttgccgtcg tctggtattg ctgaactatt ttggcgaagg
     3721 gcgtcaggag ccgtgcggga actgcgatat ctgcctcgat ccgccgaaac agtacgacgg
     3781 ttcaaccgat gctcagattg ccctttccac cattggtcgt gtgaatcagc ggtttgggat
     3841 gggttatgtg gtggaagtga ttcgtggtgc taataaccag cgtatccgcg actatggtca
     3901 tgacaaactg aaagtctatg gcatgggccg tgataaaagc catgaacatt gggtgagcgt
     3961 gatccgccag ctgattcacc tcggcctggt gacgcaaaat attgcccagc attctgccct
     4021 acaactgaca gaggccgcgc gcccggtgct gcgcggcgaa tcctctttgc aacttgccgt
     4081 gccgcgtatc gtggcgctca aaccgaaagc gatgcagaaa tcgttcggcg gcaactatga
     4141 tcgcaaactg ttcgccaaat tacgcaaact gcgtaaatcg attgccgatg aaagcaatgt
     4201 cccgccgtac gtggtgttta acgacgcaac cttgattgag atggctgaac agatgccgat
     4261 caccgccagc gaaatgctca gcgttaacgg cgttgggatg cgcaagctgg aacgctttgg
     4321 taaaccgttt atggcgctta tccgcgcgca tgttgacggc gacgacgaag agtagtcagc
     4381 ggcataaaaa agtgccagga tgaagactcc gtaaacgttt cccccgcgag tcaaatgtat
     4441 gttgatgtta tttctcaccg tcgccatggt gcatattgtg gcgcttatga gccccggtcc
     4501 cgatttcttt tttgtctctc agaccgctgt cagtcgttcc cgtaaagaag cgatgatggg
     4561 cgtgctgggc attacctgcg gtgtaatggt ttgggctggg attgcgttgc ttggcctgca
     4621 tttgattatc gaaaaaatgg cctggctgca tacgctgatt atggttggcg gtggcctgta
     4681 tctctgctgg atgggttacc agatgctacg cggtgcactg aaaaaagagg ctgtatctgc
     4741 gcctgcacca cagattgagc tggcgaaaag cgggcgcagt ttcctgaaag gtttactgac
     4801 caatctcgct aatccgaaag cgattatcta ctttggctcg gtgttctcat tgtttgtcgg
     4861 tgataacgtt ggcactaccg cgcgctgggg catttttgcg ctgatcattg tcgaaacact
     4921 ggcgtggttt accgtcgttg ccagcctgtt tgccctgccg caaatgcgcc gtggttatca
     4981 acgtctggcg aagtggattg atggttttgc cggggcgtta tttgccggat ttggcattca
     5041 tttgattatt tcgcggtgat gccagacgcg tcttcagagt aagtcggata aggcgtttac
     5101 gccgcatccg acattatttt tcacgcatgc ctcgccgatg ctaacagcgc tcccaccagc
     5161 ataaacaacg agccgaaaat cttattcagc gccttcatct gctttggtcc tttaatccat
     5221 agagcaatcc gttgagcaag ggtggcgtaa ccgatcatca caataatatc gactacaata
     5281 gtggtgacgc cgagcacgat atactgcatc agttgcggct gctgtggcat gatgaattgc
     5341 ggaaatagcg ccgccagaaa cacaatactt ttgggattgg tgagattcac aaaaactgcg
     5401 cgctggaaca aatgtcgacg cgattgagta gaggccagcg atttaaggtc aattgcacca
     5461 gcggcgcgcc actgctggat tcccagccaa atcaagtaag ccgcgcctgc ccacttcaac
     5521 acttcaaacg caatcactga gcgggaaaat agcgtcccca accccacgcc aaccagcaca
     5581 atatgaatcg ccagtccggt ctgaagccca gcaatagacg ccaccgcgcc gcgataaccg
     5641 tggttgagcg aggtggtcat agtgttgatt gcaccagagc ctggcgacag gcttaaaatg
     5701 atcgatgtca gcaggtaggc aaaccaccat tctaaggtca tgatgaactc ccggtgtgtc
     5761 tatttttgtg ccacaatacg ctactgtcgc agcgttgtgt caggcacgct aaaaaaaacg
     5821 attttacgtg gtttaagagg cagattaccc gatgtttcag cagcaaaaag actgggaaac
     5881 aagagaaaac gcgtttgctg cttttaccat gggaccgctg actgatttct ggcgtcagcg
     5941 tgatgaagca gagtttactg gtgtggatga cattccggtg cgctttgtcc gttttcgcgc
     6001 acagcaccat gaccgggtgg tagtcatctg cccggggcgt attgagagct acgtaaaata
     6061 tgcggaactg gcctacgacc tgttccattt ggggtttgat gtcttaatca tcgaccatcg
     6121 cgggcaggga cgttccggtc gcctgttagc cgatccgcat ctcgggcatg ttaatcgctt
     6181 taatgattat gttgatgatc tggcggcatt ctggcagcag gaggttcagc ccggtccgtg
     6241 gcgtaaacgc tatatactgg cacattcgat gggcggtgcg atctccacat tatttctgca
     6301 acgccatcca ggtgtatgtg acgccattgc gctaactgcg ccaatgtttg ggatcgtgat
     6361 tcgtatgccg tcatttatgg cacggcagat cctcaactgg gccgaagcgc atccacgttt
     6421 ccgtgatggc tatgcaatag gcaccgggcg ctggcgcgcg ttgccgtttg ctatcaacgt
     6481 actgacccac agcagacagc gatatcgacg taacttacgc ttctatgctg atgacccaac
     6541 gattcgcgtc ggtgggccga cctaccattg ggtacgcgaa agtattctgg ctggcgaaca
     6601 ggtgttagcc ggtgcgggtg atgacgccac gccaacgctt ctcttgcagg ctgaagagga
     6661 acgcgtggtg gataaccgca tgcatgaccg tttttgtgaa ctccgcaccg ccgcgggcca
     6721 tcctgtcgaa ggaggacgac cgttggtaat taaaggtgct taccatgaga tcctttttga
     6781 aaaggacgca atgcgctcag tcgcgctcca cgccattgtt gattttttca acaggcataa
     6841 ctcacccagc ggaaaccgct ctacagaggt ttaaatttct tatgtaccag gttgttgcgt
     6901 ctgatttaga tggcacgtta ctttctcccg accatacgtt atccccttac gccaaagaaa
     6961 ccctgaagct gctcaccgcg cacggcatca actttgtgtt tgcgaccggt cgtcaccacg
     7021 ttgatgtggg gcaaattcgc gataatctgg agattaagtc ttacatgatt acctccaacg
     7081 gcgcgcgcgt tcacgatctg gatggcaatc tgatttttgc tcataacctg gatcgcgaca
     7141 ttgccagcga tctgtttggc gtagtcaacg acaatccgga catcattact aacgtttatc
     7201 gcgacgacga atggtttatg aatcgccatc gcccggaaga gatgcgcttt tttaaagaag
     7261 cggtgttcaa atatgcgctg tatgagcctg gattactgga gccggaaggc gtcagcaaag
     7321 tgtttttcac ctgcgattcc catgaacaac tgctgccgct ggagcaggcg attaacgctc
     7381 gttggggcga tcgcgtcaac gttagtttct ctaccttaac ctgtctggaa gtgatggcgg
     7441 gcggcgtttc aaaaggccat gcgctggaag cggtggcgaa gaaactgggc tacagcctga
     7501 aggattgtat tgcgtttggt gacgggatga acgatgccga aatgctgtcg atggcgggga
     7561 aaggctgcat tatgggcagt gcgcaccagc gtctgaaaga ccttcatccc gagctggaag
     7621 tgattggtag taatgccgac gacgcggtgc cgcattatct gcgtaaactc tatttatcgt
     7681 aatcgttctt tatttggtca gttgtcaacc tgatacttcg ctacaatgga tactcgttaa
     7741 tcaaagagtt ttccattgtg gcgctactta tcatcaccac gattctgtgg gccttctcct
     7801 ttagctttta tggcgagtac cttgcggggc acgtcgatag ctattttgcg gtgctggtgc
     7861 gcgttggcct ggcggcactc gtttttctgc cgtttctgcg tacccgtggc aatagcctga
     7921 aaacagtcgg cctgtatatg ctggtgggcg cgatgcagct cggcgtgatg tatatgctga
     7981 gtttccgcgc ttatctctac ctgacggttt ccgaactgct gctgttcacc gtactgacgc
     8041 cgctctacat cacgctgatt tatgacatca tgagtaagcg ccgtctgcgc tggggatatg
     8101 cttttagtgc cttgttggcg gtgattggtg ccgggattat tcgctatgat caggtcaccg
     8161 accatttctg gactggcttg ttgctggtgc aactctccaa tatcactttt gccatcggca
     8221 tggtgggtta caaacgcctg atggaaactc gcccgatgcc gcagcataac gcctttgcgt
     8281 ggttctatct tggcgcgttc ctggtggcgg taattgcatg gttcttgctg ggaaatgcgc
     8341 agaaaatgcc gcaaaccacg ttgcaatggg gcattctggt gtttcttggc gtggtggctt
     8401 ccgggattgg ctactttatg tggaactacg gcgcgacgca ggtggacgcc ggaacgctgg
     8461 gcattatgaa taatatgcac gttccggcag ggctgctggt aaacctggct atctggcacc
     8521 aacagccgca ctggccaacg ttcattacag gcgcgctggt gatcctggcc tcactgtggg
     8581 tgcatcgtaa gtgggtcgct ccgcgctctt cacaaacggc agatgatcgc aggcgtgatt
     8641 gcgcgctgag cgaataaatg cttctgtaat cggctgacgc tgctcgccat cgcgcacggc
     8701 ggcgtacagt cggctccaca agccttcgcc cagggttttg gtcaccacca gaccctggcg
     8761 ctcaaaactc tctactaccc aatgcggtag cgcggcaata cccatccgcg cggcaaccat
     8821 ctgaatcaac aataaggtgt tatcgacgct tttcagtgac gggctgacgc ctgccggctg
     8881 aagaaaatgc cgccagacat ccagtcgact acgctgcacc ggataaatca acagcgtctc
     8941 gctggcgaga tcttccggtg taattcgcgt tttcgccgcc agtggatggt caggtgctaa
     9001 caccagacgc acttcatagt cgaacatcgg cgaataatgc aggccactgc gcggcaggat
     9061 atcggaggtc atcaccagat ccagctcgcc ctgttgcaac gcgggctgcg ggtcaaacgt
     9121 cacgcccgat ttaaaatcca tctctacctg cggccagttc ttatggaaat tttctaacgc
     9181 gggcgtcagc cactgaatac agctatggca ctcaatggca atgcgcagac gcgtctgctg
     9241 cggttcattg caggcttgca gtgcctggct aatttgcggc agcacctggt ttgccagttg
     9301 caacaggatt tctccctgcg gtgtaaagcg tagcggctgg ctcttacgca caaatagccg
     9361 gaagccaagg cgttgttcca gatcgctaaa ctggtgagac agggcggatt gcgtctgatg
     9421 caacgtcgcc gcagcggctg cgagagagcc gcagttccgc aacgcttgta gcgttttcag
     9481 gtgttttact tcgatcatga aagtccttca cttcggcatg aataatttgc gcttgaggaa
     9541 tatacagtaa ccgccaatta tggatgtgta aacatctgga cggctaaata ctaaaaattc
     9601 cataaggggc atataatgac aattcttaat cacaccctcg gtttccctcg cgttggcctg
     9661 cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg ggaactccac gcgtgaagaa
     9721 ctgctgacag tagggcgtga actgcgtgct cgtcactggg atcaacaaaa gcaagcgggt
     9781 atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgttctgac caccagtctg
     9841 ctgctgggta atgttccgcc acgtcatcag aacaaagacg gttcggtaga tatcgacacc
     9901 ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc agcggaaatg
     9961 accaaatggt ttaacaccaa ctatcactac atggtgccgg aattcgttaa aggccaacag
    10021 ttcaaactga cctggacgca gctgctggag gaagtggacg aggcgctggc gctgggccac
    10081 aacgttaagc ctgtgctgct ggggccggtt acttacctgt ggctggggaa agtgaaaggt
    10141 gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca gcaagtgctg
    10201 gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc gctggtactg
    10261 gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct ccagggacag
    10321 gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc caaatctcga cacgattact
    10381 gcgctgcctg ttcagggtct gcatgttgac cttgtacatg gtaaagatga cgttgttgaa
    10441 ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg gactgatcaa tggtcgtaac
    10501 gtctggcgcg ccgatcttac tgagaaatat gcgcaaatta aggacattgt cggcaaacgt
    10561 gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct gagcgtggaa
    10621 acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ctctacaaaa atgccatgaa
    10681 ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc agagtggagc
    10741 gccccaattc aggcgcgtcg tcactctact cgtgtacata atccggcggt agaaaagcgt
    10801 ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt gcgtgctgaa
    10861 gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg ttccttcccg
    10921 caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct cgacgccaac
    10981 aactaccgca cgggcattgc ggaacatatc aagcaggcca ttgttgagca ggaacgtttg
    11041 ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga atactttggc
    11101 gagcacctcg acggatttgt ctttacgcaa aacggttggg tacagagcta cggttcccgc
    11161 tgcgtgaagc caccgattgt cattggtgac attagccgcc cggcaccgat taccgtggag
    11221 tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga aagggatgct gacggggccg
    11281 gtgaccatac tctgctggtc gttcccgcgt gaagatgtca gtcgtgaaac catcgccaaa
    11341 cagattgcgc tggcgctgcg tgatgaggtg gccgatctgg aagcggcagg gattggcatc
    11401 atccagattg atgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag cgactgggac
    11461 gcctatctcc agtggggcgt agaggccttc cgtatcaacg ccgccgtggc gaaagatgac
    11521 acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga ttcgattgcg
    11581 gcgctggacg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga gttgctggag
    11641 tcgtttgaag agtttgatta tccaaatgaa atcggtcctg gcgtctatga cattcactcg
    11701 ccaaacgtac cgagcgtgga atggattgaa gccttgctga agaaagcggc aaaacgcatt
    11761 ccggcagagc gcctgtgggt caacccggac tgtggcctga aaacgcgcgg ctggacagaa
    11821 acccgcgcgg cactggcgaa catggtgcag gcggcgcaga atttgcgtcg ggggtaaatc
    11881 caaaccgggt ggtaatacca cccggtcttt tctcattaca gcgacttctt cccaccatac
    11941 tgcttaaacc actccagcat acgctgccag ccatctttcg cagattcggc atgatagctc
    12001 gggcgataat cggcgttgaa tgcatgcccg gcatccgggt agacgataat ctctgctttc
    12061 gcattagcag cccgcagcgc ctggcgcatg gtttcaacgc tctcctgcgg aatgctgtta
    12121 tcctgaccac catataagcc gagaaccggc gcgttaagat cggttgcgat atcaacaggt
    12181 tgtttcggtg aattcagcga cttgtcgccc gtcagtttgc cgtaccacgc cactgcagct
    12241 tttagctgtg gattatgcgc ggcatacagc caggtgatac gtccacccca gcagaatccg
    12301 gtgatcatta aacgatgaac atcgccgccg ttgcgggaag cccaactggc gacatgatcg
    12361 agatcggcca gcacctgcga gtcaggcact tttgctacca gaccgctaag caacgtgggg
    12421 atatcggcaa aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc gatagccaga
    12481 tacccctcca gcgccagacg gcgacaaatg tcgcggatat gttcatgcac gccaaaaatt
    12541 tcctgcacta caatgaccac tggcagtggg ccatcgcttt gctttggtct ggcatggtaa
    12601 gcaggcatgt tatccccttg tgaagggata gaggtgaagc ccgccacaat cgcgtcgtcc
    12661 ggggtctgaa cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc agattgttgt
    12721 gttgttgcca tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt aactatagtc
    12781 agcatgcaac aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc tgcagaatga
    12841 agagtgattt atgtgatttg aatcactttt ggtgggtaaa tttatgcaac gcatttgcgt
    12901 catggtgatg agtatcacga aaaaatgtta aacccttcgg taaagtgtct ttttgcttct
    12961 tctgactaaa ccgattcaca gaggagttgt atatgtccaa gtctgatgtt tttcatctcg
    13021 gcctcactaa aaacgattta caaggggcta cgcttgccat cgtccctggc gacccggatc
    13081 gtgtggaaaa gatcgccgcg ctgatggata agccggttaa gctggcatct caccgcgaat
    13141 tcactacctg gcgtgcagag ctggatggta aacctgttat cgtctgctct accggtatcg
    13201 gcggcccgtc tacctctatt gctgttgaag agctggcaca gctgggcatt cgcaccttcc
    13261 tgcgtatcgg tacaacgggc gctattcagc cgcatattaa tgtgggtgat gtcctggtta
    13321 ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt cgcaccgctg gaattcccgg
    13381 ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc tgcgaaatcc attggcgcga
    13441 caactcacgt tggcgtgaca gcttcttctg ataccttcta cccaggtcag gaacgttacg
    13501 atacttactc tggtcgcgta gttcgtcact ttaaaggttc tatggaagag tggcaggcga
    13561 tgggcgtaat gaactatgaa atggaatctg caaccctgct gaccatgtgt gcaagtcagg
    13621 gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg cacccagcaa gagatcccga
    13681 atgctgagac gatgaaacaa actgaaagcc aagcggtgaa aatcgtggtg gaagcggcgc
    13741 gtcgtctgct gtaattctct tctcctgtct gaaggccgac gcgttcggcc ttttgtattt
    13801 ttgcgtagcg cctcgcagga aatgcctttc caactggacg tttgtacagc acaattctat
    13861 tttgtgcggg taagttgttg cgtcaggagg cgttgtggat ttctcaatca tggtttacgc
    13921 agttattgcg ttggtgggtg tggcaattgg ctggctgttt gccagttatc aatatgcgca
    13981 gcaaaaagcc gagcaattag ctgaacgtga agagatggtc gcggagttaa gcgcggcaaa
    14041 acaacaaatt acccaaagcg agcactggcg tgcagagtgc gagttactca ataacgaagt
    14101 gcgcagcctg caaagtatta acacctctct ggaggccgat ctgcgtgaag taaccacgcg
    14161 gatggaagcc gcacagcaac atgctgacga taaaattcgc cagatgatta acagcgagca
    14221 gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt tttgagcaca gcaatcgccg
    14281 ggttgatgag caaaaccgtc agagtctgaa cagcctgttg tcgccgctac gtgaacaact
    14341 ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa gaagcacaag aacgccatac
    14401 cctgacccac gaaattcgca atctccagca actcaacgcg caaatggccc aggaagcgat
    14461 caacctgacg cgcgcgctga aaggcgacaa taaaacccag ggcaactggg gcgaggtagt
    14521 attgacgcgg gtgctggagg cttccggtct gcgtgaaggg tatgaatatg aaacccaggt
    14581 cagcatcgaa aatgacgccc gctcgcggat gcagccggat gtcatcgtgc ggctgccgca
    14641 gggaaaagat gtggtgatcg acgccaaaat gacgctggtc gcctatgaac gctattttaa
    14701 cgccgaagac gactacaccc gcgaaagcgc gctacaggaa catatcgcgt cggtgcgtaa
    14761 ccatatccgt ttgctgggac gcaaagatta tcaacagctg ccggggctgc gaactctgga
    14821 ttacgtgctg atgtttattc ccgttgaacc cgctttttta ctggcgcttg accgccagcc
    14881 ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg gttagcccga ctacgctgct
    14941 ggtggcgttg cgtactattg ccaacctgtg gcgttatgag catcaaagcc gcaacgccca
    15001 gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg cgtttgttca tcgatgacat
    15061 gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac taccgccagg caatgaaaaa
    15121 actctcttca ggtcgcggaa atgtgctggc gcaggcagaa gcgtttcgcg gtttaggagt
    15181 agaaattaaa cgcgagatta atccggattt ggctgaacag gcggtgagcc aggatgaaga
    15241 gtatcgactt cggtcggttc cggagcagcc gaatgatgaa gcttatcaac gcgatgatga
    15301 atataatcag cagtcgcgct agcccattag gggtagttaa gccgggtaga aatctagggc
    15361 atcgacgccc aatctgttac acttctggaa caattttttg atgagcaggc attgagatgg
    15421 tggataagtc acaagaaacg acgcactttg gttttcagac cgtcgcgaag gaacaaaaag
    15481 cggatatggt cgcccacgtt ttccattccg tggcatcaaa atacgatgtc atgaatgatt
    15541 tgatgtcatt tggtattcat cgtttgtgga agcgattcac gattgattgc agcggcgtac
    15601 gccgtgggca gaccgtgctg gatctggctg gtggcaccgg cgacctgaca gcgaaattct
    15661 cccgcctggt cggagaaact ggcaaagtgg tccttgctga tatcaatgaa tccatgctca
    15721 aaatgggccg cgagaagctg cgtaatatcg gtgtgattgg caacgttgag tatgttcagg
    15781 cgaacgctga ggcgctgccg ttcccggata acacctttga ttgcatcacc atttcgtttg
    15841 gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc aatgtatcgc gtgctgaaac
    15901 ccggcggccg cctgctggtg cttgagttct cgaagccaat tatcgagccg ctgagcaaag
    15961 cctatgatgc atactccttc catgtgctgc cgcgtattgg ctcactggtc gcgaacgacg
    16021 ccgacagcta ccgttatctg gcagaatcca tccgtatgca tcccgatcag gataccctga
    16081 aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta ctacaatctg acggcagggg
    16141 ttgtggcgct gcatcgtggt tataagttct gacaggagac cggaaatgcc ttttaaacct
    16201 ttagtgacgg caggaattga aagtctgctc aacaccttcc tgtatcgctc acccgcgctg
    16261 aaaacggccc gctcgcgtct gctgggtaaa gtattgcgcg tggaggtaaa aggcttttcg
    16321 acgtcattga ttctggtgtt cagcgaacgc caggttgatg tactgggcga atgggcaggc
    16381 gatgctgact gcaccgttat cgcctacgcc agtgtgttgc cgaaacttcg cgatcgccag
    16441 cagcttaccg cactgattcg cagtggtgag ctggaagtgc agggcgatat tcaggtggtg
    16501 caaaacttcg ttgcgctggc agatctggca gagttcgacc ctgcggaact gctggcccct
    16561 tataccggtg atatcgccgc tgaaggaatc agcaaagcca tgcgcggagg cgcaaagttc
    16621 ctgcatcacg gcattaagcg ccagcaacgt tatgtggcgg aagccattac tgaagagtgg
    16681 cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg aagagacggc tgccgtcgag
    16741 cgtgctgttg atgccctgac caaacggctg gaaaaactgg aggctaaatg acgccaggtg
    16801 aagtacggcg cctatatttc atcattcgca cttttttaag ctacggactt gatgaactga
    16861 tccccaaaat gcgtatcacc ctgccgctac ggctatggcg atactcatta ttctggatgc
    16921 caaatcggca taaagacaaa cttttaggtg agcgactacg actggccctg caagaactgg
    16981 ggccggtttg gatcaagttc gggcaaatgt tatcaacccg ccgcgatctt tttccaccgc
    17041 atattgccga tcagctggcg ttattgcagg acaaagttgc tccgtttgat ggcaagctgg
    17101 cgaagcagca gattgaagct gcaatgggcg gcttgccggt agaagcgtgg tttgacgatt
    17161 ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt tcataccgcg cgattgaaat
    17221 cgaatggtaa agaggtggtg attaaagtca tccgcccgga tattttgccg gttattaaag
    17281 cggatctgaa acttatctac cgtctggctc gctgggtgcc gcgtttgctg ccggatggtc
    17341 gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa gacattgatt gatgaactga
    17401 atttgctgcg ggaatctgcc aacgccattc agcttcggcg caattttgaa gacagcccga
    17461 tgctctacat cccggaagtt taccctgact attgtagtga agggatgatg gtgatggagc
    17521 gcatttacgg cattccggtg tctgacgttg cggcgctgga gaaaaacggc actaacatga
    17581 aattgctggc ggaacgcggc gtgcaggtgt tcttcactca ggtctttcgc gacagctttt
    17641 tccatgccga tatgcaccct ggcaacatct tcgtaagcta tgaacacccg gaaaacccga
    17701 aatatatcgg cattgattgc gggattgttg gctcgctaaa caaagaagat aaacgctatc
    17761 tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg caaagtggca gagctacacg
    17821 tcgattcagg ctgggtgcca ccagatacca acgttgaaga gttcgaattt gccattcgta
    17881 cggtctgtga acctatcttt gagaaaccgc tggccgaaat ttcgtttgga catgtactgt
    17941 taaatctgtt taatacggcg cgtcgcttca atatggaagt gcagccgcaa ctggtgttac
    18001 tccagaaaac cctgctctac gtcgaagggg taggacgcca gctttatccg caactcgatt
    18061 tatggaaaac ggcgaagcct ttcctggagt cgtggattaa agatcaggtc ggtattcctg
    18121 cgctggtgag agcatttaaa gaaaaagcgc cgttctgggt cgaaaaaatg ccagaactgc
    18181 ctgaactggc ttacgacagt ttgcgccagg gcaagtattt acagcacagt gttgataaga
    18241 ttgcccgcga gcttcagtca aatcatgtac gtcagggaca atcgcgttat tttctcggaa
    18301 ttggcgctac gttagtatta agtggcacat tcttgttggt cagccgacct gaatgggggc
    18361 tgatgcccgg ctggttaatg gcaggtggtc tgatcgcctg gtttgtcggt tggcgcaaaa
    18421 cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg tataatgcgg ccttgtttaa
    18481 tcatcatcta ccacagagga acatgtatgg gtggtatcag tatttggcag ttattgatta
    18541 ttgccgtcat cgttgtactg ctttttggca ccaaaaagct cggctccatc ggttccgatc
    18601 ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga tgatgaacca aagcaggata
    18661 aaaccagtca ggatgctgat tttactgcga aaactatcgc cgataagcag gcggatacga
    18721 atcaggaaca ggctaaaaca gaagacgcga agcgccacga taaagagcag gtgtaatccg
    18781 tgtttgatat cggttttagc gaactgctat tggtgttcat catcggcctc gtcgttctgg
    18841 ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg ctggattcgc gcgttgcgtt
    18901 cactggcgac aacggtgcag aacgaactga cccaggagtt aaaactccag gagtttcagg
    18961 acagtctgaa aaaggttgaa aaggcgagcc tcactaacct gacacccgaa ctgaaagcgt
    19021 cgatggatga actacgccag gccgcggagt cgatgaagcg ttcctacgtt gcaaacgatc
    19081 ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc ggtggtgaaa gataatgaag
    19141 ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca ggccagttcg ccggaacaga
    19201 agccagaaac cacgccagag ccggtggtaa aacctgctgc ggacgctgaa ccgaaaaccg
    19261 ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg tctgtagaag atactcaacc
    19321 gcttatcacg catctgattg agctgcgtaa gcgtctgctg aactgcatta tcgcggtgat
    19381 cgtgatattc ctgtgtctgg tctatttcgc caatgacatc tatcacctgg tatccgcgcc
    19441 attgatcaag cagttgccgc aaggttcaac gatgatcgcc accgacgtgg cctcgccgtt
    19501 ctttacgccg atcaagctga cctttatggt gtcgctgatt ctgtcagcgc cggtgattct
    19561 ctatcaggtg tgggcattta tcgccccagc gctgtataag catgaacgtc gcctggtggt
    19621 gccgctgctg gtttccagct ctctgctgtt ttatatcggc atggcattcg cctactttgt
    19681 ggtctttccg ctggcatttg gcttccttgc caataccgcg ccggaagggg tgcaggtatc
    19741 caccgacatc gccagctatt taagcttcgt tatggcgctg tttatggcgt ttggtgtctc
    19801 ctttgaagtg ccggtagcaa ttgtgctgct gtgctggatg gggattacct cgccagaaga
    19861 cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc gttgtcggga tgttgctgac
    19921 gccgccggat gtcttctcgc aaacgctgtt ggcgatcccg atgtactgtc tgtttgaaat
    19981 cggtgtcttc ttctctcgct tttacgttgg taaagggcga aatcgggaag aggaaaacga
    20041 cgctgaagca gaaagcgaaa aaactgaaga ataaattcaa ccgcccgtca gggcggttgt
    20101 catatggagt acaggatgtt tgatatcggc gttaatttga ccagttcgca atttgcgaaa
    20161 gaccgtgatg atgttgtagc gcgcgctttt gacgcgggag ttaatgggct actcatcacc
    20221 ggcactaacc tgcgtgaaag ccagcaggcg caaaagctgg cgcgtcagta ttcgtcctgt
    20281 tggtcaacgg cgggcgtaca tcctcacgac agcagccagt ggcaagctgc gactgaagaa
    20341 gcgattattg agctggccgc gcagccagaa gtggtggcga ttggtgaatg tggtctcgac
    20401 tttaaccgca acttttcgac gccggaagag caggaacgcg cttttgttgc ccagctacgc
    20461 attgccgcag atttaaacat gccggtattt atgcactgtc gcgatgccca cgagcggttt
    20521 atgacattgc tggagccgtg gctggataaa ctgcctggtg cggttcttca ttgctttacc
    20581 ggcacacgcg aagagatgca ggcgtgcgtg gcgcatggaa tttatatcgg cattaccggt
    20641 tgggtttgcg atgaacgacg cggactggag ctgcgggaac ttttgccgtt gattccggcg
    20701 gaaaaattac tgatcgaaac tgatgcgccg tatctgctcc ctcgcgatct cacgccaaag
    20761 ccatcatccc ggcgcaacga gccagcccat ctgccccata ttttgcaacg tattgcgcac
    20821 tggcgtggag aagatgccgc atggctggct gccaccacgg atgctaatgt caaaacactg
    20881 tttgggattg cgttttagag tttgcggaac tcggtgttct tcacactgtg cttaatctct
    20941 ttattaataa gattaagcaa tagcatggag cgagcctcac catcgggttc ggtgaaaatg
    21001 gcctgaaagc cttcgaacgc gccttcggta ataatcacct tatcacccgg ataaggggtt
    21061 gccggatcga caatgtcttt cggtttatat accgatagct gatgaatcac cgccgatggg
    21121 actatcgctg gcgacgcgcc aaagcgcacg aagtggctga caccgcgggt cgcgttgata
    21181 gtcgtggtat gaatcacttc tggatcaaat tcgacaaaca ggtagttggg aaacaatggc
    21241 tcactgactg cagtacgttt tccacgcacg attttttcca gggtgatcat cggtgccagg
    21301 caattcacag cctgtctttc gaggtgttcc tgggcacgtt gaagttgccc gcgcttgcag
    21361 tacagtaaat accaggattg cataatgact cttatccgtt taatcggggc gcaaggatag
    21421 caaaagcttt acgctaagtt aattatattc cccggtttgc gttataccgt cagagttcac
    21481 gctaatttaa caaatttaca gcatcgcaaa gatgaacgcc gtataatggg cgtagattaa
    21541 gaggctacaa tggacgccat gaaatataac gatttacgcg acttcctgac gctgcttgaa
    21601 cagcagggtg agctaaaacg tatcacgctc ccggtggatc cgcatctgga aatcactgaa
    21661 attgctgacc gcactttgcg tgccggtggg cctgcgctgt tgttcgaaaa ccctaaaggc
    21721 tactcaatgc cggtgctgtg caacctgttc ggtacgccaa agcgcgtggc gatgggcatg
    21781 gggcaggaag atgtttcggc gctgcgtgaa gttggtaaat tattggcgtt tctgaaagag
    21841 ccggagccgc caaaaggttt ccgcgacctg tttgataaac tgccgcagtt taagcaagta
    21901 ttgaacatgc cgacaaagcg actgcgtggt gcgccctgcc aacaaaaaat cgtctctggc
    21961 gatgacgtcg atctcaatcg cattcccatt atgacctgct ggccggaaga tgccgcgccg
    22021 ctgattacct gggggctgac cgtaacgcgc ggcccgcata aagagcggca gaatctgggc
    22081 atttatcgtc agcagctgat tggtaaaaac aaactgatta tgcgctggct gtcgcatcgc
    22141 ggcggcgcgc tggattatca ggagtggtgt gcggcgcatc cgggcgaacg tttcccggtt
    22201 tctgtggcgc tgggtgccga tcccgccacg attctcggtg cagtcacccc cgttccggat
    22261 acgctttcag agtatgcgtt tgccggattg ctacgtggca ccaagaccga agtggtgaag
    22321 tgtatctcca atgaccttga agtgcccgcc agtgcggaga ttgtgctgga agggtatata
    22381 gaacaaggcg aaactgctcc ggaagggccg tatggcgacc acaccggtta ctataacgaa
    22441 gtcgatagtt tcccggtatt taccgtgacg catattaccc agcgtgaaga tgcgatttac
    22501 cactccacct atactggtcg tccgccggat gaacccgcgg tgctgggtgt cgcactgaac
    22561 gaagtgtttg tgccgattct gcaaaaacag ttcccggaaa ttgtcgattt ttacctgccg
    22621 ccggaaggct gttcttatcg tctggcggta gtgacgataa aaaaacagta cgccggacac
    22681 gcgaagcgcg tcatgatggg cgtctggtcg ttcttacgcc agtttatgta cactaagttt
    22741 gtgatcgttt gcgatgatga cgtcaacgca cgcgactgga acgatgtgat ttgggcgatt
    22801 accacccgta tggatccggc gcgggatact gttctggtag aaaatacgcc tattgattat
    22861 ctggattttg cctcgcctgt ttccgggctg ggttcaaaaa tggggctgga tgccacgaat
    22921 aaatggccgg gggaaaccca gcgtgaatgg ggacgtccca tcaaaaaaga tccagatgtt
    22981 gtcgcgcata ttgacgccat ctgggatgaa ctggctattt ttaacaacgg taaaagcgcc
    23041 tgatgtgcgt ttgttttgcc ctatttatcg atccgacaga gaaagcgcat gacaacctta
    23101 agctgtaaag tgacctcggt agaagctatc acggataccg tatatcgtgt ccgcatcgtg
    23161 ccagacgcgg ccttttcttt tcgtgctggt cagtatttga tggtagtgat ggatgagcgc
    23221 gacaaacgtc cgttctcaat ggcctcgacg ccggatgaaa aagggtttat cgagctgcat
    23281 attggcgctt ctgaaatcaa cctttacgcg aaagcagtca tggaccgcat cctcaaagat
    23341 catcaaatcg tggtcgatat tccccacgga gaagcgtggc tgcgcgatga tgaagagcgt
    23401 ccgatgattt tgattgcggg cggcaccggg ttctcttatg cccgctcgat tttgctgaca
    23461 gcgttggcgc gtaacccaaa ccgtgatatc accatttact ggggcgggcg tgaagagcag
    23521 catctgtatg atctctgcga gcttgaagcg ctttcgttaa agcaccctgg tctgcaagtg
    23581 gtgccggtgg ttgaacaacc ggaagcgggc tggcgtgggc gtactggcac cgtgttaacg
    23641 gcggtattgc aggatcacgg tacgctggca gagcatgata tctatattgc cggacgtttt
    23701 gagatggcga aaatagcccg tgacctgttt tgtagtgagc gtaatgcgcg ggaagatcgc
    23761 ctgtttggcg atgcgtttgc atttatctga gatataaaaa aacccgcccc tgacaggcgg
    23821 gaagaacggc aactaaaact ttattcagtg gcatttagat ctatgacgta tctggcaaac
    23881 catgcccgat gcgacgctgt cgcgtcttat cgtgcctaca aatagtccga accgtaggcc
    23941 ggataaggcg tttacgccgc atccggcaag tggttaaacc cgctcaaaca ccgtcgcaat
    24001 accctgaccc agaccgatac acatcgtcgc cagaccaaac tgaacgtctt tgcgttccat
    24061 cagattcagc agcgtggtgc tgatacgcgc accggaacag cccagcggat gacccagcgc
    24121 gatcgcacca ccattgaggt tgatcttctc gtcaatctgc tccattagcc cgagatcttt
    24181 aatgcacggc aggatctgcg cggcaaaggc ttcgttcatc tcaaacacgc cgatatcgct
    24241 ggcagaaagc cccgcttttt tcagcgccag cttcgaggcc ggaaccgggc cgtaacccat
    24301 aatcgatggg tcacaaccaa cgaccgccat cgaacgcaca cgagcgcgcg gcttaagacc
    24361 taattcatgg gcgcggcttt cactcatcac cagcatggca gctgcgccat cggaaagtgc
    24421 agaagatgtg cccgccgtta ccgtaccgtt tactggatca aacgccggac gcagcgtggc
    24481 gagggcttcc acggtggttt ccgggcgaat cacttcgtcg taattaaact gcttcaggac
    24541 gccgtcggca tcgtgaccac cagttgggat gatttcattt ttaaatgcgg ccgactgcgt
    24601 ggcggcccag gcgcgggcgt gtgaccgcgc ggcaaaggca tcctgcattt cacggctgat
    24661 accgtgcata cgcgccagca tttctgctgt taagcccatc atgcccgccg ctttggcgac
    24721 attgcggctc aggccggggt gaaaatcgac gccgtgactc atcggcacat ggcccatatg
    24781 ctccacgccg ccaaccagac atgcctgcgc atcgccggtc atgatcattc gtgctgcgtc
    24841 atgcagtgcc tgcatggatg aaccacacaa gcgattgacg gtaaccgccg ggacagagtg
    24901 cggcacttct gccagcagcg ccgcgttacg ggcgatgtta aaaccctgct ccagcgtctg
    24961 ctgcacacaa ccccagtaaa tatcgtcgag ggctgccgct tccagcgccg ggttacgcgc
    25021 cagcaggcta cgcattaaat gagcggagag atcttccgca cgcacgttac gaaaagcacc
    25081 gcccttcgaa cggcccatcg gggtgcgaat tgcatcgaca atgacaacct gttccattgt
    25141 gactccttaa gccgttttca ggtcgccaac cggacgggct ggctcaaccg ggggatagta
    25201 cggttcgtta tgacgtgctt tattacgcag accttccggc acttcgtaca gcgggccgag
    25261 gtgctgatat tgctgtgcca tatcgaggta ttttgcgcta ccgagggtgt ccagccagcg
    25321 gaacgcgccg ccgtggaacg gagggaagcc caggccgtag accagcgcca tatccgcttc
    25381 cgccggagtg gcgataatgc cttcctccag acagcgcacc acttcgttga ccatcgggat
    25441 catcatgcgg gcgataatct cttcttcgct gaaatcgcgc ttcggctggc tcacttctgc
    25501 cagcaggtct tcaacggcgg cgtcttcttc tttcttcggc ttacctttgc tgtcttcttt
    25561 ataacgccag aaaccgaggc cgttcttctg accaaagcgg ttggcatcaa acagcgcgtc
    25621 gatggcatcg cggtaatctt tctgcatccg ctgcgggaag cctgctgcca tgacagcctg
    25681 agcgtgatgc gcggtatcaa tgcccacgac atccagcaga tatgccggac ccataggcca
    25741 gccaaactgt ttttccatca ctttgtcgat cttgcggaaa tccgcgccgt cgcgcagcag
    25801 ctggctgaaa ccggcgaaat acgggaacag cacgcggtta acaaagaagc cggggcagtc
    25861 gttaaccaca atcggcgtct tgcccatctt gctcgcccag gcgacaactt tcgcgatggt
    25921 ttcgtcggag cttttctcgc cgcgaataat ttctaccaac ggcatccggt gtaccgggtt
    25981 aaaaaagtgc attccgcaga agttttccgg gcgttccagc gcgttggcca gttcgctgat
    26041 aggaatggtt gaagtgttag acgccagcac ggtatccggg cgtacttttt gttcggtttc
    26101 tgccagtacg gcttttttca ctttcgggtt ttcgacaacc gcttctacca caacatccac
    26161 gcggtcaaac ccggcgtagt cgagcgttgg gtggattgtg gagatcacgc cagccagttt
    26221 cagaccatcg atcttgccgc gctcaagctg cttgttcagc agtttcgcgg cttcggtcat
    26281 gccgagggtt aacgacttgt cgttgatatc tttcatgaca accggcacgc ctttccacgc
    26341 agactggtaa gcgatgccgc cgcccataat gcctgcaccc agcaccgcgg cctgtttcgg
    26401 ggtttcaacg tctttggtga gtttcttcgc tttgcctttt acatattgat cgttaaggaa
    26461 aatgccgacc agtgcgcggg cttcgttggt atgcgccagc gggacaaaac ttttgttttc
    26521 caggtttaag gcttcttcac gaccaaaacg ggccgcagct tcaatggttt ttactgcggt
    26581 gatgggggcc ggataatgtt tccccgctgt ttgtgcgacc atccctttag cgatggtgaa
    26641 gctcatggtg gcttcaatct tgctcagttt tagtggttcc agcttcggct gacgttttgc
    26701 tttccagtcg aggtcgccgt taatggcctg gcgtaaaacc gcctttgcgc cttcaaccag
    26761 tttttctgct ttgactacgc catccaccag accgattttc agcgcctgat ccgcgccgac
    26821 atctttaccg gcggcaatga tttccagcgc actgtcagcg cccagcatac gtggcatacg
    26881 tacagaaccg ccaaagcccg gcatgatacc cagtttggtt tccggcagac cgatgcgcag
    26941 atccggcgtc gccagacgat aatcggtcgc cagcacgcat tcgcagccac cgcccagcgc
    27001 atagccgttg acggcagcaa tggtcggcac cggcagatct tccaggcgat taaacacgct
    27061 attggcaaat tgcagccact gacttaactg ttcttcagga acgaggaaca gggacaaaaa
    27121 ttcggtgata tcagcaccga cgataaaggc tgctttgttc gaacgcagca gcagcccttt
    27181 tagatctgat tgctgttcca gcacgccgat ggcctcgccg aggctggcga cggtcgcagt
    27241 gtcgagttta ttaactgaac ctggggcatc aaataccagt tcggcaatgc catcttccag
    27301 ccagtcaagg tacagggtgt cgcctttgta aagcatgtca gtctcctgaa tccgcaaggt
    27361 gatctggtcg taccagatga gtcgaagtgt gtattttgtg ttaaaaatat gcaaacaaaa
    27421 gattaaagaa atgccgatct gatcacgctc ggcagaaatc acgctctgga tgaacgatgt
    27481 gctaagatgc ggagacttaa ggtcaaaaaa acagaagggt aaaaaatgga atcactggcc
    27541 tcgctctata aaaatcatat agctacctta caagaacgga ctcgcgatgc gctggcgcgc
    27601 ttcaagctgg atgcgttact tattcactcc ggcgagctgt tcaatgtttt tctcgacgat
    27661 catccctatc cgtttaaagt gaacccgcaa ttcaaagcgt gggtgccggt aactcaggtg
    27721 ccaaactgct ggttgctggt ggatggcgtg aacaagccga aactgtggtt ttatctgccg
    27781 gttgattact ggcacaacgt cgaaccgctg ccgacctcct tctggactga agatgtggaa
    27841 gtgatcgcgc tgccgaaagc cgatggcatt ggtagcctgc tgcctgctgc gcgcggcaat
    27901 atcggttata tcggtccggt gccggagcgt gcgctgcaac tgggtattga ggccagcaac
    27961 atcaacccga aaggggttat cgactacctg cattactatc gctccttcaa aaccgagtac
    28021 gaactggcct gtatgcgtga agcgcagaaa atggcggtca acggtcatcg tgcggcagaa
    28081 gaagcgttcc gttctggcat gagcgagttc gatatcaaca ttgcctatct gaccgcgacc
    28141 ggtcatcgtg ataccgacgt accttacagc aacattgtgg cgcttaacga acacgctgcg
    28201 gtgctgcatt acaccaaact ggatcatcag gcgccggaag agatgcgcag cttcctgctg
    28261 gatgccgggg ccgaatataa cggctatgcg gctgacctga ctcgtacctg gtcggcaaaa
    28321 agcgacaacg actacgcaca gctggtgaaa gacgtaaatg atgaacaact ggcgctgatc
    28381 gccaccatga aagcaggcgt tagctatgtg gattaccaca tccagttcca tcagcgcatc
    28441 gccaaactgc tgcgtaaaca tcaaatcatc accgatatga gtgaagaagc gatggtcgaa
    28501 aacgatctca ccggaccgtt tatgccgcac ggtatcggtc atccgctggg cctgcaggtg
    28561 catgacgtag ccggttttat gcaggatgat agcggtacac acctcgcggc accggcaaaa
    28621 tatccgtacc tgcgctgcac ccgtattctc cagccgggca tggtgttaac catcgaaccg
    28681 ggtatctact tcattgaatc gctactggca ccgtggcgtg aagggcagtt cagcaagcac
    28741 ttcaactggc agaaaattga agcactgaaa ccgttcggcg gcattcgtat cgaagacaac
    28801 gtggtgatcc acgaaaacaa cgtggaaaac atgacccggg atctgaaact ggcgtgatgg
    28861 aaagctggtt aattcctgcg gcaccggtca cggtcgttga agagatcaaa aagagccgct
    28921 tcataacact gttggcgcat accgatggcg ttgaggcggc gaaagcgttt gttgaatcgg
    28981 tgcgggcaga acaccccgat gcccgccacc attgcgtggc gtgggtggcg ggtgcgccgg
    29041 atgattctca acagctgggt ttctctgacg acggggagcc ggcaggaacg gcaggtaaac
    29101 cgatgctcgc ccagctaatg ggcagcggcg tcggggaaat taccgctgtg gtagtgcgct
    29161 actacggtgg catattgctt ggcaccggtg ggttagtgaa agcgtatggc ggcggcgtga
    29221 atcaggcgct gcgccagcta acgacccaac gcaagacgcc attaaccgaa tatactttgc
    29281 aatgtgaata tagtcagtta accggcattg aagcgttgct ggggcagtgt gacggcaaaa
    29341 ttatcaacag tgattatcag gcattcgttc tgctgcgggt ggcgcttccg gcggcgaaag
    29401 tggctgaatt ttccgcaaag ctggcggatt ttagccgtgg ttcattgcaa ttgttagcga
    29461 ttgaagaata atccccactt cgttttgcag aactaaggaa gcggcagaga tgcattttcg
    29521 cgccattacc cgaatcgttg gactactggt catcttattt tcagggacca tgattatccc
    29581 tgggctggta gcactcatct accgggatgg agcgggccgc gcttttaccc agaccttttt
    29641 tgtcgccctc gccattggtt ctatgctgtg gtggccgaac cgtaaagaga agggcgaact
    29701 gaaatcccgt gaggggtttc tgatagtggt gctgttctgg accgtgctgg gtagtgtcgg
    29761 tgcgctccct tttatcttct cggaaagccc gaacctcacg attaccgatg cgttttttga
    29821 atctttctct ggcctgacca ccaccggggc cactacgctg gtggggctgg attcgctccc
    29881 tcacgccatc ctcttttatc gccagatgct gcaatggttt ggcgggatgg ggatcatcgt
    29941 gttagcggtt gcgatactgc ctatcctcgg cgtgggtggg atgcagctct atcgcgcaga
    30001 aatgcccggc ccgctgaaag ataacaaaat gcgcccgcga attgcggaaa cggcgaaaac
    30061 cctgtggttg atttatgtct tgctgaccgt cgcctgtgcg ctggcgttgt ggtttgccgg
    30121 aatggatgcc tttgatgcca tcggccatag ctttgcgact atcgctattg gcggcttctc
    30181 gacacatgat gccagtatcg gttatttcga cagcccgact attaacacta tcattgctat
    30241 cttcctgctg atctccggct gtaactacgg tctgcacttt tcactgttaa gtgggcgtag
    30301 tctgaaggtt tattggcgcg atccggaatt tcgcatgttt atcggcgtac agtttacgct
    30361 ggtggttatt tgtaccctcg tactgtggtt tcacaatgtc tacagttcgg cgctgatgac
    30421 aattaaccag gcgtttttcc aggtggtgtc gatggcgaca accgccgggt ttacgactga
    30481 cagcattgcc cgctggccgc tctttttgcc ggtactgctt ttatgttcag cttttatcgg
    30541 cggttgtgcc gggtcaacgg gcggtggcct gaaagtgatc cgcatcctgc tgctgtttaa
    30601 gcaggggaac cgtgagctga aacgactggt gcatccgaac gccgtctata gcattaagct
    30661 ggggaatcgc gcactgccgg aacgtatcct cgaagccgtt tggggatttt tctccgccta
    30721 tgcattggtg tttattgtca gtatgctggc gattatcgcc acgggcgtgg atgacttttc
    30781 tgcttttgcc tcggttgttg cgacattgaa taacctgggg ccggggcttg gcgtggttgc
    30841 tgataacttt accagtatga acccggtggc taaatggatc ctgattgcca acatgctgtt
    30901 tggtcgtctc gaggtcttta cattgctggt gctctttacc ccgactttct ggcgtgaatg
    30961 atggagtaat acgtgaaaac attaattctt ttctcaacaa gggacggaca aacgcgcgag
    31021 attgcctcct acttggcttc ggaactgaaa gaactgggga tccaggcgga tgtcgccaat
    31081 gtgcaccgca ttgaagaacc acagtgggaa aactacgacc gtgtggtcat tggtgcttct
    31141 attcgctatg gtcactacca ctctgcgttc caggaatttg tcaaaaagca tgcgacgcgg
    31201 ctgaattcga tgccgagcgc cttttactcc gtaaacctgg tggcgcgtaa accggagaag
    31261 cgcactccac agaccaacag ctacgcgcgg aagtttctga tgaactcgca atggcgtccc
    31321 gatcgctgcg cggtcattgc cggggcgctg cgttacccac gttatcgctg gtacgaccgt
    31381 tttatgatca agctaattat gaagatgtca ggcggtgaaa cggatacgcg caaagaagtt
    31441 gtctataccg attgggagca ggtggcgaat ttcgcccgag aaatcgccca tttaaccgac
    31501 aaaccgacgc tgaaataagc ataaagaata aaaaatgcgc ggtc
//
LOCUS       DBANUU010000049        31015 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000049 DBANUU010000000
VERSION     DBANUU010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.049"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACIE68_002129"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACIE68_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFX5908440.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACIE68_002130"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACIE68_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFX5908441.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACIE68_002131"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACIE68_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFX5908442.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACIE68_002132"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACIE68_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFX5908443.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACIE68_002133"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACIE68_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFX5908444.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACIE68_002134"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACIE68_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFX5908445.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACIE68_002135"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACIE68_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFX5908446.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACIE68_002136"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACIE68_002136"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFX5908447.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACIE68_002137"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACIE68_002137"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFX5908448.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACIE68_002138"
     CDS             12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACIE68_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HFX5908449.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACIE68_002139"
     CDS             complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACIE68_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFX5908450.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACIE68_002140"
     CDS             complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACIE68_002140"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFX5908451.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETCAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACIE68_002141"
     CDS             complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACIE68_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFX5908452.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACIE68_002142"
     CDS             complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACIE68_002142"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFX5908453.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACIE68_002143"
     CDS             complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACIE68_002143"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFX5908454.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACIE68_002144"
     CDS             complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACIE68_002144"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFX5908455.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19283..19383)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACIE68_002145"
     CDS             complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACIE68_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFX5908456.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20006..20779
                     /gene="nudC"
                     /locus_tag="ACIE68_002146"
     CDS             20006..20779
                     /gene="nudC"
                     /locus_tag="ACIE68_002146"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFX5908457.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20819..21883
                     /gene="hemE"
                     /locus_tag="ACIE68_002147"
     CDS             20819..21883
                     /gene="hemE"
                     /locus_tag="ACIE68_002147"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFX5908458.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLVRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21893..22564
                     /gene="nfi"
                     /locus_tag="ACIE68_002148"
     CDS             21893..22564
                     /gene="nfi"
                     /locus_tag="ACIE68_002148"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFX5908459.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22607..23197
                     /gene="yjaG"
                     /locus_tag="ACIE68_002149"
     CDS             22607..23197
                     /gene="yjaG"
                     /locus_tag="ACIE68_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFX5908460.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23384..23656
                     /gene="hupA"
                     /locus_tag="ACIE68_002150"
     CDS             23384..23656
                     /gene="hupA"
                     /locus_tag="ACIE68_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFX5908461.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23669..24364
                     /gene="yjaH"
                     /locus_tag="ACIE68_002151"
     CDS             23669..24364
                     /gene="yjaH"
                     /locus_tag="ACIE68_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFX5908462.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="ACIE68_002152"
     CDS             complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="ACIE68_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFX5908463.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25029..26405
                     /gene="zraS"
                     /locus_tag="ACIE68_002153"
     CDS             25029..26405
                     /gene="zraS"
                     /locus_tag="ACIE68_002153"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFX5908464.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26402..27727
                     /gene="zraR"
                     /locus_tag="ACIE68_002154"
     CDS             26402..27727
                     /gene="zraR"
                     /locus_tag="ACIE68_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFX5908465.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27724..29013)
                     /gene="purD"
                     /locus_tag="ACIE68_002155"
     CDS             complement(27724..29013)
                     /gene="purD"
                     /locus_tag="ACIE68_002155"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFX5908466.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVEDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29025..30614)
                     /gene="purH"
                     /locus_tag="ACIE68_002156"
     CDS             complement(29025..30614)
                     /gene="purH"
                     /locus_tag="ACIE68_002156"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFX5908467.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKLDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPSEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7314 a         8043 c         8476 g         7182 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacac cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggcaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttagtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gtatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca
     5101 tcttttgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag
     5341 cgtatcgaaa cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacttgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcact acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctc tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaagtga ccgacggtgt tgtaactgac gaaattcact acctgtctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgcga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgacagacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtatatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcgggttaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggggcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gtactgggtc tgtactacat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcta acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg ttaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaagggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaata ctgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 tcagaccatt actcgtcaga cagacgaatt gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gcacgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtggggg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatggggg gagtttaggg aaagagcatt tgtcagaata tttaaggaat ttctgaatac
    12781 tcataatcaa tgtagagatg actaatatcc tgaaactgac tgaactaatt gagtcaaact
    12841 cggcaaggat tcgatactat tcctgtgtaa ctttcttaag gaacgagaat gaaacaggaa
    12901 gtggaaaagt ggcgaccttt tggacatccg gatggtgata ttcgtgattt atcatttctt
    12961 gatgctcatc aggctgtcta cgttcagcat catgagggca aagagccttt agagtatcgc
    13021 ttttgggtta cctactctct tcactgcttc acaaaagatt atgaacatca gacgaacgaa
    13081 gaaaaacaat cgttaatgta ccacgcgcct aaagaatctc gtcccttctg ccagcaccgt
    13141 tataacttag cgcgcacaca cttaaaaaga actattttgg cgctgccaga aagcaacgtt
    13201 attcatgccg ggtatggtag ctatgccgtg attgaggtgg acttagacgg aggagataag
    13261 gcattttact ttgttgcgtt cagggctttc agggaaaaga aaaaactccg tttgcatgta
    13321 actagcgctt atcccatttc tgaaaaacag aaaggtaaat cagtgaaatt tttcaccatt
    13381 gcctacaact tattgagaaa taagcagctt cctcagccct caaaataaca aaacccacct
    13441 taaggtgggt ttcgccagag aattatctct ggtattcaga acgccattac cggactttgc
    13501 cttgaccttg cgataatcgc aggttgcggg atgtctgaat ttcttcagtc tgctgcatcc
    13561 tggaagatga gaacatgtgt tcttattttc gtctctatca tagttgagta tttactctct
    13621 tacaatcaga tctctttcat tgctcaacag gcgatggctt cagactttgc attacggaat
    13681 ttttaagaaa ggcagggcga aacgaggaag aagcttttcg ccctggacaa cattcctgct
    13741 gacggcacta ccataaaaaa acagaacgct ttcaacaacc ttgcgttttt catcttccgt
    13801 agcggctcgg aataaattta cgttcttaca ttccgtctca tagtctttgc gaggcccgtc
    13861 ccagatagct gtcccagtct ttccataccg gctgcaaacc ctgagcggtt aacgcggcag
    13921 caaccgcttc cggtctgcga tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat
    13981 cggcatagcc acctggctgc gttttcgaga aggcgctgac gttattgatc gccagcggaa
    14041 taacgcgatc gcgaaaccac ggtgattccc gcgtggagag tgacagttca atctccggtg
    14101 caagcagtcg gaaggcgcag atggtttgca ctaactggcg ttcatccata atcgacgcag
    14161 gctcaatgcc gccagtacac gggcgcaggc gcggaaagga gacagagtaa cggctttgcc
    14221 agtaatgctg ttgcagccat agcaaatgtt cggcaaccat atagcagtca acgcgccagt
    14281 tgtcggaaag gccaattagc gcgccgaggc ctatcttatc aatccccgca cgccccagcc
    14341 gatccggcgt ttccagccgc cagaagaagt cctgtttttt gcctttcaga tgatggcggg
    14401 cataagtcgc ctcgtgatat gtctcctgat aaaccatcac gccatccaga ccaagctgct
    14461 ttaactcggc gtattccgtc tccgccagcg gttgcacttc catttgtagt gaagagaact
    14521 gttcacgcag ggcggggaga tgacgacgaa agtaatccat ccccactttc gcctgatgtt
    14581 caccagtgac taatagcaga tgttcaaagc ccatctcccg tatagcggcg ctttccctgg
    14641 caatatccgc ttcatccagc gttttgcgct tgatgcgatt actcatggaa aatccgcagt
    14701 acgtgcagtc gttagcgcaa agattggaaa gataaagcgg gacgtagaaa ctaactgtgt
    14761 tgccaaatcg ctgacgggtc agacgctgcg cccgttgggc cagttgttcc agatagccac
    14821 tggcggctgg cgataacagc gccatcatat cgtcgcgggt gaattgcgag gcatttagcg
    14881 cccgctctac gtcaacagcc gttttgccgt tgatacgcag gcggatgtca tcccagtcca
    14941 gttgtcgcca gcgatcgctg aaggttttca tgccgatgcc tccagaaatc cagtcagcgg
    15001 gctggtggca tgagcaaaat gactgcggct gcccggtccg gactgacgtg ccagcaggcc
    15061 tgcttctacc gccagacgaa atgccttcgc catgttgacg ggatcgtccg ctacggcaat
    15121 cgccgtattc actaacaccg cgtcggcccc catttccagg gcctgcgcgg catggctggg
    15181 aacgccgatg ccagcatcga caaccaccgg aactgtggcc tgctggataa taatctccag
    15241 catggcacag gtttccagtc cctgattcga gccaatcggc gcgccgagcg gcatcaccgc
    15301 tgcacagccg acttcttcca gacgtttaca caataccgga tcggccccgc agtaaggcag
    15361 cacgacaaat ccctgttgta ccagcatttc ggcggctttc agagtttcga tgggatcggg
    15421 caacagccag cgggcgtcag ggtgaatctc caattttagc cagttagtgc ctaacgcttc
    15481 acgtgccaga tgggcggcga aaatggcttc ttccgctgtt ttcgccccgg atgtatttgg
    15541 cagcagggtc acacccgccg cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa
    15601 gtcgacacgt ttcatcgcca gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac
    15661 catcagttgt gaagaagcga atttccctgt gccggtaaac agatgtgaat caaacgtttt
    15721 gtccgcaata cgtaacattt caaccccctg caataacctg aaaaagcagg atctggtcgc
    15781 catcctgcac gatatgttgc gcccactgct cacgcgggac gatttgctga ttaatcgcca
    15841 gagccgcgcc cgcttgtcgt tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg
    15901 cggcgcactg catcggttga tcgttaaaca ggatctgcat tgcttcctcc gcataccggg
    15961 caaccactgg cgcggcgcaa cgccaggctg cgccactggc tcgatttacc gtcgaacagt
    16021 cggagttctc ccgcaggtgt ctctataccg cttaataact taatggcttc cagtgcctgc
    16081 aaagtgccca taaccccgac caccgggcca accacgcccg ccgtgcggca gttgcgttct
    16141 ggctcctggt tatctggcca caggcagcgg taacacccct gctcccaggg cggcgtcagt
    16201 accatcaact gaccgccaaa tccgaccgcg ctggcggtga taagcggcgt gttgagtgcc
    16261 acgcaggcgg cattaatctc ctggcgagtc gccatattgt cggtacagtc gagcaccaca
    16321 tcggcccgtg caaccgcatc ttttaacgcc tcacccgtta accgttgttg taatgctgtc
    16381 agttgaatgt cgggattcaa ctgtgtcagt cgctgttggc tgacctgcga tttcgggcga
    16441 tcgatatctt cagtggtaaa gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg
    16501 tcatctgcca gtaccagcgt cccgacgcca gcgcccgcca ggtacagcgc agcaggtgta
    16561 cccagcccgc ccagaccgat aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc
    16621 agagcgatat cgtcgagcag gatttggcgg ctataacgca taaagtcacg gtcattcatc
    16681 gccaactcct gcaatttcca gcaactgtgc cgttgccaaa cgccagtctg cagcttgagt
    16741 aatggcgctg acgacggcga tactgccgac acccgttgct atcaccgcag gcgcgcgtgc
    16801 cagactgata ccgccaatcg ccacggtggg ataatccgcc agtcgctcaa catgccgtgc
    16861 cagctgttcc agcccctgcg gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg
    16921 tcccagcgcg atataagagg ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg
    16981 tgtcgaaacg cccagccgca ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa
    17041 atcttcctgc cccaaatgga cgccatacgc ctgatgcttg atcgccagcc gccagtaatc
    17101 gttgataaac aatcgcgcgt tatagcggcg gcccagcgca attgccgcca cgacatcggc
    17161 ttccacctct tcatcgcgcc gatctttgat gcgtagctgg agagtacgta cgcctgcatc
    17221 caacagacgt tcgatccact gtacgctgtc caccaccggg tacagtccta aacgaaaagg
    17281 tacaggagga aaatcaggct gatacatcac gcttcctcct tacgcaggta gatttctccg
    17341 cctctggcac ggaagttctc cgacatatcc gccattccca tttcaatagt ttgcgtggcg
    17401 gcgtaatcac gcacttcctg gctgattttc atcgagcaaa atttcggtcc acacatggag
    17461 caaaaatggg cgactttgcc ggactcttgg ggcagggttt catcgtgata agcgcgggcg
    17521 gtaaacgggt cgagggccag attaaactgg tcttcccagc gaaattcgaa gcgggctttc
    17581 gacatggcgt tatcgcgaat ttgcgcgccc ggatgccctt tcgccaggtc agcggcgtgg
    17641 gcggcaatct tataggtgat aagcccctgc ttaacatctt ctttattagg cagacccaga
    17701 tgctcttttg gcgttacgta acagagcatc gcgcagccaa accagccaat catcgccgca
    17761 ccaatccccg acgtgaagtg gtcatagccc ggcgcaatat cggtagttag cggccccaga
    17821 gtgtaaaacg gcgcttcgtg gcagtgctct aactcctcgg tcatattgcg gcggatcatc
    17881 tgcatcggca cgtggcctgg gccttcaatc atcacctgca cgtcatattc ccaggcaatt
    17941 ttggtcagtt cgcccagcgt atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga
    18001 atagaaccgg ggcgcagacc gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt
    18061 tcacaaattt cgcggaagtg ttgatagagg aaattttcct gatgatggga gaggcaccat
    18121 ttcgccataa tcgaaccgcc gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc
    18181 acatagcgca gcagtacgcc cgcatggata gtgaagtaat ccacaccttg ctcggcctgt
    18241 tccagcagcg tgtcgcggaa cgcttcccag gtaagatctt cggcgatccc gttaaccttc
    18301 tccagcgcct ggtagatcgg cactgtaccg atcggcaccg ggctgttacg caaaatccac
    18361 tcgcgggttt cgtgaatata gcgaccggtg gagagatcca tcaccgtatc cgctccccag
    18421 cgcgtggacc ataccagctt ttccacttct tcttcgatgg aagaggtgac cgccgagttg
    18481 ccgatattgg cgttaacttt taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc
    18541 ggatgattaa tgttggccgg gataatcgca cgtccggcag caacttcatc acggacaaat
    18601 tccgcagtga tattttccgg cagatgtgcg ccaaagctca ttcccggatg ctggtggcgt
    18661 aaaacctcgc tacggatgcg ctcgcggccc atattctcgc ggatggcgat gaattccatt
    18721 tccggcgtga tgatgccctg gcgggcgtag tgcagttggg tgacacggcg tcctgctttg
    18781 gcgcgttttg gtgttagtac gccgctaaaa cgcagttcgt cgaggccatc atctgccagc
    18841 cgcgctttag tgtaatcgga actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg
    18901 atccacggct ggcgtagttt tgccagccct tgctgcacgt taatggcaat ctgcggatca
    18961 ccatacgggc cggaggtgtc gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt
    19021 tctttgctac cgccaattag cgtcgggcta agctggatct cacgcatcgg cacgcgcacg
    19081 ccggggtgtg tgccagtgat ataaatgcgt tttgagttgg gaaaggcggt gccttccagg
    19141 gtgtcgataa aatgttgggc ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac
    19201 atagctcatt ccaaaaagtt aaggacgtgg cttgtcagac gacggatgaa gcaagagacg
    19261 atcgccgcag gggcgatgcg atagcagatt aactcttgtt cccttcgcag gtattagcct
    19321 gatcaggttc cgcggatccc gaataaacgg tctcagccag ttaaggcact ccgacaagaa
    19381 attagccccg caaatggggc attgaatgta aattacgcgt taacagcgca gaactcaagc
    19441 aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc agcttatctt ccagcacaaa
    19501 gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt tgaaactcaa ggtaattatc
    19561 atgatcgatg gcggtttcca gactggaatc gtagtaatcc ataatctgtt gagtattggc
    19621 ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc ccgttgcctt ccagcttatg
    19681 aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag taatcgacca ggctctgaca
    19741 aaaatcatca agggcttttt cgtttagcct catgtacgat tctttgccag gcttaatgcc
    19801 aaccagattg tagtaagcca cgagcagatg cttacgtaca tgtagccagc gatcaaccag
    19861 tttgttactt cctctgacgc gctccgtcag gttatcgagc tggttaagca tgattgactc
    19921 cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa aaaccatgtt aaacatgcca
    19981 gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt gaaaaattag atcacggctg
    20041 gtgggtcgtc agccatgaac aaaaattatg gttgccgaag ggagaattgc catatggcga
    20101 agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa atcggcgaat ggcaggggga
    20161 acctgtttgg ttagtacaac agcagcggcg tcacgatatg gggtcggtac gtcaggtcat
    20221 tgatctcgat gttgggctgt ttcaactggc cggacgaggc gtacaactgg cggagtttta
    20281 ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg tatccgagca aaaccgaatg
    20341 ggcgatgctg tgcagccatt gccgtgagcg ttactacccg caaatcgccc cctgcattat
    20401 tgttgccatc cgtcgcgatg attcgatcct cctcgcccag catacccgcc atcgtaacgg
    20461 tgtccataca gtacttgccg gattcgtcga agtgggcgaa accctcgagc aggcagtcgc
    20521 gcgggaagtg atggaagaga gcggaattaa agttaaaaac ttgcgttacg tgacttctca
    20581 gccgtggccg tttcctcagt ctttaatgac cgcgtttatg gcggaatatg acagcggcga
    20641 catcgtgatc gacccgaaag aattgctcga ggcgaactgg tatcgctatg acgatttgcc
    20701 gttactcccg ccgcccggca ccgtagcgcg ccgtctgata gaagatacgg tggcgatgtg
    20761 tcgggcagag tatgagtgat gatacactga ccgcctgacg cactaaggaa cagccaaaat
    20821 gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg cgccagcccg ttgatgtcac
    20881 tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg gaatataaag ccacgcgcgc
    20941 ccaggcgggc gattttatgt cgctgtgcaa aaacgccgag ctggcgtgcg aagtgacttt
    21001 gcaaccgctg cgtcgctacc cgctggatgc ggcgatcctc ttttccgata tcctcaccgt
    21061 gccggacgcg atggggttag ggctctattt tgaagccgga gaaggtccgc gttttacctc
    21121 gccagtcacc tgcaaagccg acgtcgataa actgccaatt ccggacccgg aagatgaact
    21181 gggttacgtg atgaacgcgg tgcgtaccat tcgtcgcgaa ctgaaaggcg aagtgccgct
    21241 gattggtttt tccggcagcc cgtggacgct ggcgacctac atggtggaag gcggcagcag
    21301 caaagccttc accgtgatca aaaaaatgat gtatgccgat ccgcaggcgc tgcacgctct
    21361 gctcgataaa ctggcgaaaa gcgtcacttt gtatctgaat gcgcagatta aagccggtgc
    21421 tcaggcagtg atgattttcg acacctgggg cggcgtgctt accgggcgcg attatcaaca
    21481 gttctcgctc tattacatgc ataaaattgt tgatggttta gtgcgtgaaa acgacggtcg
    21541 ccgcgtaccg gtcacgctgt ttaccaaagg cggcggacag tggctggaag ccatggcaga
    21601 aaccggttgc gatgcgctgg gcctcgactg gacaacggac atcgccgatg cgcgccgccg
    21661 tgtgggcaat aaagtcgcgt tgcagggtaa tatggatccg tcgatgctgt acgcgccgcc
    21721 tgcccgcatt gaagaagaag tagcgactat acttgcaggt ttcggtcacg gcgaaggtca
    21781 tgtctttaac cttggtcacg gcattcatca ggatgtgccg ccagaacatg ctggcgtgtt
    21841 cgtggaggca gtgcatcgac tgtctgaaca gtatcaccgc taaggagtga ttatggatct
    21901 cgcgtcatta cgcgctcaac aaatcgaact ggcttcttct gtgatccgcg aggatcgact
    21961 cgataaagat ccaccggatc tgatcgccgg agccgatgtc gggtttgagc agggcggaga
    22021 agtgacgcga gcggcgatgg tgctgctgaa atatccctcg cttgagctgg tggagtataa
    22081 agttgcccgc atcgccacca ccatgcctta cattccaggt tttctttcct tccgcgaata
    22141 tcctgcgctg ctggcagcgt gggagatgct gtcgcaaaag ccggatttag tgtttgtcga
    22201 tggtcatggg atctcgcatc ctcgccgtct tggcgtcgcc agccattttg gcttattggt
    22261 ggatgtgccg accattggcg tggcgaaaaa acgtctctgc ggtaaattcg aaccgctctc
    22321 cagcgaaccg ggcgcgctgg ccccgctgat ggataaaggc gagcagctgg cctgggtctg
    22381 gcgcagcaaa gcgcgctgta acccgttgtt tatcgctacc ggccatcggg tcagcgtgga
    22441 cagcgcgctg gcgtgggtac aacgctgcat gaaaggctat cgtctgccgg agccaacgcg
    22501 ctgggcggac gcggtggcct cggaacgtcc ggcgttcgtg cgctatacag caaatcagcc
    22561 ctaattcagg taaactgcgg ataatttccg tatttgagaa ctcaacatgt tacaaaaccc
    22621 aattcatctg cgtctggagc gcctggaaag ctggcagcat gtcactttca tggcttgctt
    22681 atgcgaacgc atgtacccca attacgccat gttctgccag caaaccggtt ttggtgatgg
    22741 gcaaatttac cgtcgtattc tcgatctcat ctgggaaacg ctgaccgtta aagacgcaaa
    22801 agtaaatttc gacagccaac tggagaaatt tgaagaagcg attccttcag ccgacgattt
    22861 cgatctgtac ggcgtttatc cggcaatcga tgcctgcgtg gcgttaagtg aactggtcca
    22921 ttcgcgtttg agtggtgaaa cgctcgaaca cgcggtggaa gtgagtaaga cctccatcac
    22981 gaccgttgcg atgctggaaa tgactcaggc tggtcgcgaa atgagcgatg aagagcttaa
    23041 agaaaaccca gctgtagagc aagaatggga cattcagtgg gaaatattcc gacttttagc
    23101 tgagtgcgaa gaacgcgata tcgagctgat aaaaggcctt agggcagacc tgcgtgaggc
    23161 gggtgagagc aatattggta taatttttca gcaataagac cagaaaacgt gatttaacgc
    23221 ctgatttgtc gtacctggag tcttcccttt cgccccccgt ctggtctaca tttggggggc
    23281 gaaaaaaagt ggctatcggt gcgtgtatgc aggagagtgc tattctggca tttccgtcgc
    23341 actcgatgct tagcaagcga taaacacatt gtaaggataa cttatgaaca agactcaact
    23401 gattgatgta attgcagaga aagcagaact gtccaaaacc caggctaaag ctgctctgga
    23461 gtccactctg gctgcaatta ctgagtctct gaaagaaggc gatgctgtac aactggttgg
    23521 tttcggtacc ttcaaagtga accaccgcgc tgagcgtact ggccgcaacc cgcagaccgg
    23581 taaagaaatc aaaattgccg cagctaacgt accggcattt gtttctggca aggcactgaa
    23641 agacgcagtt aagtaagatt gcgtggcagt gaacagtttt aacgaagggg tggtttcacc
    23701 ccttttgtct ttctggcgtc gatcattgat gctggcaggt gcgctgtttc tcactgcctg
    23761 tagccacaac tcttcacttc ctccctttac cgccagtgga tttgctgaag accagggcgc
    23821 ggtacgcatc tggcgaaaag acagcggcga taatgtgcat ctgcttgccg tgtttagccc
    23881 gtggcgcagt ggcgatacca cgacgcgaga gtatcgctgg cagggcgata acctcacgct
    23941 catcaatatc aatgtttaca gcaaaccgcc ggtgaacatt cgcgcgcgtt ttgacgatcg
    24001 cggtgatctg agctttatgc aacgtgaatc cgatggggaa aagcagcagc tttctaacga
    24061 ccaaatcgat ttataccgtt atcgtgctga tcagatccgc cagattagcg atgccttacg
    24121 tcaggggaga gtcgtgctgc gccaggggcg ctggcatgcg atggaacaga ccgtgaccac
    24181 ctgcgaaggg caaaccatta aacctgattt agattcgcag gcgatagcgc atatcgagcg
    24241 ccgccagagc cgctcttctg ttgatgtcag cgtggcatgg ctggaagcgc ccgaaggttc
    24301 gcaattactg ttagtggcaa actctgattt ctgtcgctgg caacccaacg agaaaacgtt
    24361 ctgatttacc agtggcccat acccatatga ccgccaccac cgcagccgcc gtagcccatg
    24421 cccattccgg ctccgcgcgg aatacccgct tcagccatcg cgatatctcg tttcacccgt
    24481 aactcatcta acgactgacg caaattctcc atctctttgg cgaccgcgtt aattttgctg
    24541 ctatccggtg ggttcgcggc taacagggca ttgtattcat aacgcttcgt caccagttgc
    24601 tgttgcagtg cgctgctttg agcgtaaaag tcattatgga ttttctgcca cgctgtctgt
    24661 tgttcgctgg tcaaaggcgc ggcattttgc tgccacatac cgtgtccgcc gtgagcaaat
    24721 gcagatgtcg atcccatcgc cattgctgaa agcgccatca ttaccagggc aattttcgtg
    24781 ttccgtttca tggttaatcc tccagtggtt gtcttacttc gggtattgca tcttccgtgc
    24841 caacgatgaa acgctgatat gacgggtaat ctggcatgat aaacgagtaa aaatgactcg
    24901 cctgctgcgg gtagcgagtc atttttactc attgaaactt aagcctttgt gttacagcgc
    24961 agggtaagcg ctgataaaag atggcatgat ttctgctgtc agaaagggat gagcaggcaa
    25021 agaagaagat gcgttttatg caacgttcta aagactcctt agctaaatgg ttaagcgcga
    25081 tcctccccgt ggtcattgtt gggctggtag ggttgtttgc ggtgacggtg attcgcgatt
    25141 atgggcgcga gactgccgcc gccagacaaa cgctgctgga aaaaggcagt gtacttattc
    25201 gcgctcttga atccggctcg cgcgtcggca tggggatgcg catgcatcat gcgcagcagc
    25261 aggccttact ggaagaaatg gccgggcagc ctggagtacg ttggtttgcg gtcacggatg
    25321 aacaaggaac aatcgtgatg catagcaact ccggcatggt gggaaaacag ctttattccc
    25381 cgcaggaaat gcagcagtta catcctggag atgaagaagc gtggcggcgg atcgatagcg
    25441 cagacggcga gcctgttctg gaaatttatc gccagtttca accgatgttt gctgctggaa
    25501 tgcaccggat gcgccatatg cagcagtatg ccgcgacacc acaagcaatt ttcatcgctt
    25561 tcgacgccag taatattgtg agtgccgaag atcgtgagca gagaaacacc ctgattatcc
    25621 tcttcgccct ggcgacggtc ttgctggcaa gcgtattgtc attcttctgg tatcgccgct
    25681 atctgcgctc gcgccagctt ctacaagatg aaatgaagcg caaagagaag ctggtggcgc
    25741 tggggcatct tgcggcaggc gttgcccacg aaatccgtaa cccactttcc tcgattaaag
    25801 gactggcgaa atactttgcc gaacgcgcgc cagcaggggg agaagcgcat caattggcgc
    25861 aggtgatggc gaaagaagcc gaccgtttaa accgcgtggt aagcgagttg ctggaactgg
    25921 ttaagccaac gcatctggct ttgcaggcgg tggatctcaa cacgctgatt aaccactcat
    25981 tacagctggt aagccaggat gcaaacagcc gggagatcca gttacgcttt accgccaacg
    26041 acacattacc ggaaattcag gccgatccgg acaggctgac tcaggtcctg ttgaatctct
    26101 atctcaatgc tattcaggcg attggtcagc atggcgtgat tagcgtgacg gtcagcgaaa
    26161 gcggcgcggg cgtgaaaatc agcgttaccg acagcggtaa gggaattgcg gcagatcagc
    26221 ttgaagccat cttcactccg tacttcacca ccaaagccga aggcaccgga ttggggctgg
    26281 cggtcgtgca taatattgtt gaacaacacg gtggtacaat tcaggtcgca agccaggagg
    26341 gaaaaggctc aacgttcacc ctctggcttc cggtcaatat tacgcgtaag gacccacaag
    26401 gatgacgcac gataatatcg atattctggt ggtggatgat gacattagcc actgcactat
    26461 tttgcaggct ttactgcgcg gctggggcta taacgtcgcg ctggcgaaca gcgggcgaca
    26521 ggcgctggag caggtgcggg aacaggtttt tgatcttgtg ctttgcgatg tgcgaatggc
    26581 ggagatggac ggcatcgcca cgctgaaaga gatcaaagcg ttaaacccgg caattccggt
    26641 gctgattatg actgcgtact ccagcgtcga gacggcggta gaggcactga aaactggggc
    26701 gctggattat ctcatcaagc cgctggattt cgataaccta caggcgacgc tggaaaaagc
    26761 gctcgcgcat acgcacagta ttgatgctga aacacctgcg gtgactgcca gccagttcgg
    26821 tatggtcggt aaaagcccgg cgatgcaaca cctgctcagt gaaatcgccc tcgtcgcgcc
    26881 atcggaagcc acggtactga tccacggcga ttccggcacc ggtaaagagc tggtcgccag
    26941 ggcgattcac gccagtagcg cacgtagcga aaaaccgctg gtaacgctca actgtgcggc
    27001 actcaacgaa tccttactgg aatctgaatt gttcggtcac gaaaaagggg cgtttactgg
    27061 agccgataaa cgccgggaag ggcgctttgt tgaggcggac ggcggcacgc tgtttctcga
    27121 tgaaattggc gatatctcgc cgatgatgca ggtgcgtctg ctgcgtgcga ttcaggagcg
    27181 cgaagttcag cgtgtcggta gcaaccagac tatctcggtt gatgtccggc tgattgcggc
    27241 gacccatcgc gatcttgccg cagaggtgaa tgccgggcgt tttcgccagg atctctacta
    27301 tcgcctgaat gtggtggcga ttgaagtacc atcgctgcgg caacggcggg aagatatccc
    27361 tctgctggct ggccattttc tgcagcgctt tgccgagcgt aatcgcaagg cggtaaaagg
    27421 ttttacgccc caggcgatgg atctgttgat tcattacgac tggccgggaa atattcgtga
    27481 gctggaaaac gcggtggaac gggcagtggt gctgctgacc ggggaatata tttccgaacg
    27541 cgagctgccg ctggcgattg ccagtacgcc gatcccgctg ggacaaagtc aggatattca
    27601 gccgttggtg gaagtggaaa aagaggtgat tctggcggcg ctggagaaaa cgggcggcaa
    27661 caaaaccgaa gccgcccgtc agttagggat cacgcgcaaa acgctattgg caaaactgtc
    27721 gcgttagttc tgctcgcgtt cgatagcgcg ccagccgata tctttccggc agaagcagtc
    27781 gtcccagtga atatcggtca ttaaggcata agcgcgtttc tgcgcttctg ccacggtatg
    27841 acccagcgcg gtgacgcaca gtacgcgccc gccgctggtc actacctgct cgtcatccgc
    27901 cagttttgtg cctgcatgga acactttgcc gtcttccact tcttccagcg gcaggccgtg
    27961 gatcacatca ccggtgcggt aatctcccgg atatccaccc gcagccatca ccacgccgag
    28021 agaagcgcgt tcatcccact cggatgtttt ctcgtccagc ttcccttcac aggctgccag
    28081 gcagagctca accagatcgg acttcatgcg cagcataatc ggctgggttt ccggatcgcc
    28141 aaagcggcag ttaaattcga taaccttcgg attgccctgt ttgtcgatca tcaggcccgc
    28201 gtagagaaaa ccggtgtagg tgttgccttc cgccgccatg cctttcacgg ttggccagat
    28261 gatacgttcc atggtgcgct gatgaacttc atcggttact accggcgcgg gggagtaagc
    28321 gcccatcccg ccggtgtttg gtccggtatc tttatcgcct acgcgtttgt gatcctggct
    28381 ggtagccatc ggcagcacat gctctccgtc caccatcacg ataaagctcg cttcttcgcc
    28441 atcgaggaac tcttcgataa cgatgcgatg acccgcgtcg ccaaaagcgt tgcccgccag
    28501 catatcgtga acagccgctt ccgcttcttc cagcgtcatc gccacgataa cgcctttccc
    28561 ggcagccaga ccgtccgctt taatgacgat tggcgcgcct ttctcacgca gatacgccag
    28621 cgcaggttct acctcggtga agttctggta ttccgccgta gggatcttat ggcgggccag
    28681 gaaatctttg gtaaacgctt ttgagccttc cagttgggcc gcacctgcgg ttgggccgaa
    28741 gattttcagc ccggcggcgc ggaaggtatc gaccacgcct ttcaccagcg gcgcttccgg
    28801 gccgacgatg gtcagatcaa tcttttcgtt ttgtgcgaaa tccaacagcg ccgggatatc
    28861 ggttacgccg atagcgacgt tctgtagcgc gggttccagc gcagtgcctg cattacccgg
    28921 agcaacaaaa acagtttcaa ccagcggcga ctgcgccgct ttccaggcca gcgcgtgctc
    28981 gcgcccgccg ttaccaatca ctaatacttt catctattgc tccattaatg gcggaagtgg
    29041 cgcatgtcgg tgaagagcat cgcaataccg tgctcgtcgg cggcggcaat cacttcgtca
    29101 tcacggatag aaccgccagg ctggattacg caggtcacgc ccgcagcggc ggcggcatca
    29161 ataccgtcgc ggaacgggaa gaacgcgtca gaagccatcg aggaaccttt cacttccagg
    29221 ccttcatcgg ccgctttaat accggcgatt tttgcggagt acacgcggct catctggccc
    29281 gcgccaatgc cgatagtcat attgtttttg gcatagacga tagcgttgga tttcacaaac
    29341 ttcgccacct tccagcagaa cagcgcatca cgcagttcct gttcgctcgg ctgacgtttg
    29401 gtcaccacgc gcagttcttc cgcaccgacc atccccaggt cacgatcctg aaccagcaga
    29461 ccgccgttca cgcgtttgaa atcgaggccc ggaacacgct cgccccactg accgcaggtc
    29521 agaacgcgta cgttctgttt ggcggcggtg attttcaggg cttcttcgct ggcggacggc
    29581 gcaataatca cttcgacaaa ctgacgagaa atgatggcct gtgcggtttc cgcatccagc
    29641 tcgcggttaa aggcaatgat gccgccgaat gcggaggttg ggtcggtttt gtacgcgcga
    29701 tcgtaagcat caagaatgga attgccgata gccacgccgc aagggttggc gtgcttcaca
    29761 atcacacatg ccggctcggc gaactctttc acgcactcca gcgccgcatc ggtatcggcg
    29821 atgttgttat aagagagggc tttaccctga acctgggttg cggtagcaac ggaggcttct
    29881 ttcacattct cttctatata gaaggcagcc tgctggtggc tgttctcgcc gtaacgcata
    29941 tccagcttct taatgaagtt caggttcagc gtgcgtggga agcgaccggc ggcttctttg
    30001 ctttcaccgt ggtaagccgg aaccatgctg ccgaagtagt tggcaatcat gctgtcgtag
    30061 gcggcagtgt gttcgaaggc tttgatggcg aggtcgaaac gggttgcaag cgtcagcgat
    30121 ccttcgttgt catccatctc tttaataatg gcgtcgtagt cgctgctctt caccacgatt
    30181 gcgacatctt tatggttctt ggcggcggag cgcaccatcg ttgggccgcc gatatcgatg
    30241 ttctcaaccg catcttccag cgagcaacct tcacgggcca cggtctgggc gaacggatac
    30301 aggttaacaa ccaccatatc gataggctgg atctgatgtt cttccataat ggcatcgtcc
    30361 tggccgcgac ggcccagaat gccaccatgt actttcggat gcagggtctt cacgcgtcca
    30421 tccatcatct ccgggaaacc ggtgtaatcg gaaacttcgg ttaccggcag acctttttct
    30481 gctaacagac gggcagtgcc ccctgtagac agcagctcca caccgcgtgc ggaaagtgcc
    30541 tgggcgaatt cgacgatacc ggctttgtca gaaacactga gcagagcgcg gcggactgga
    30601 cgacgttgtt gcatggtaaa tcccctggat ttgactatta cagagagcgt tagctgaatt
    30661 tttcgcgaaa aactcagcta acgcccctaa cggggcatcc ttatttttcg cccgcattgt
    30721 aacgaaaacg tttgcgcaac gctcgcgaat ttttctcttt caatggtgat cacaattttg
    30781 acagtggtta ccgtgggaaa aatacagaaa ttacattgat gattgtggat aactctgtgc
    30841 gtaaaaaggt ataaagcggg gttttgctgg ggaatgcagc agtcagttat ttttctgcaa
    30901 tttttctatt gcggcctgcg gagaactccc tataatgcgc ctccatcgac acggcggatg
    30961 tgaatcactt cacacaaaca gccggttcgg ttgaagagaa aaatcctgaa attca
//
LOCUS       DBANUU010000050        30613 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000050 DBANUU010000000
VERSION     DBANUU010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.050"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..256)
                     /locus_tag="ACIE68_002157"
     CDS             complement(<1..256)
                     /locus_tag="ACIE68_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFX5908468.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKRE"
     gene            complement(381..2828)
                     /gene="glgP"
                     /locus_tag="ACIE68_002158"
     CDS             complement(381..2828)
                     /gene="glgP"
                     /locus_tag="ACIE68_002158"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFX5908469.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2847..4280)
                     /gene="glgA"
                     /locus_tag="ACIE68_002159"
     CDS             complement(2847..4280)
                     /gene="glgA"
                     /locus_tag="ACIE68_002159"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFX5908470.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(4280..5575)
                     /gene="glgC"
                     /locus_tag="ACIE68_002160"
     CDS             complement(4280..5575)
                     /gene="glgC"
                     /locus_tag="ACIE68_002160"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFX5908471.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5593..7566)
                     /gene="glgX"
                     /locus_tag="ACIE68_002161"
     CDS             complement(5593..7566)
                     /gene="glgX"
                     /locus_tag="ACIE68_002161"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFX5908472.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7563..9749)
                     /gene="glgB"
                     /locus_tag="ACIE68_002162"
     CDS             complement(7563..9749)
                     /gene="glgB"
                     /locus_tag="ACIE68_002162"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFX5908473.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(10022..11125)
                     /gene="asd"
                     /locus_tag="ACIE68_002163"
     CDS             complement(10022..11125)
                     /gene="asd"
                     /locus_tag="ACIE68_002163"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFX5908474.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            11318..11911
                     /gene="yhgN"
                     /locus_tag="ACIE68_002164"
     CDS             11318..11911
                     /gene="yhgN"
                     /locus_tag="ACIE68_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFX5908475.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11968..13308)
                     /gene="gntU"
                     /locus_tag="ACIE68_002165"
     CDS             complement(11968..13308)
                     /gene="gntU"
                     /locus_tag="ACIE68_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFX5908476.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     FAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(13312..13839)
                     /gene="gntK"
                     /locus_tag="ACIE68_002166"
     CDS             complement(13312..13839)
                     /gene="gntK"
                     /locus_tag="ACIE68_002166"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFX5908477.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13978..14973)
                     /gene="gntR"
                     /locus_tag="ACIE68_002167"
     CDS             complement(13978..14973)
                     /gene="gntR"
                     /locus_tag="ACIE68_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFX5908478.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(15197..15892)
                     /gene="yhhW"
                     /locus_tag="ACIE68_002168"
     CDS             complement(15197..15892)
                     /gene="yhhW"
                     /locus_tag="ACIE68_002168"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFX5908479.1"
                     /translation="MIYFRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPNSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(16015..17052)
                     /gene="yhhX"
                     /locus_tag="ACIE68_002169"
     CDS             complement(16015..17052)
                     /gene="yhhX"
                     /locus_tag="ACIE68_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFX5908480.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHLTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKAIIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            17385..17873
                     /gene="yhhY"
                     /locus_tag="ACIE68_002170"
     CDS             17385..17873
                     /gene="yhhY"
                     /locus_tag="ACIE68_002170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFX5908481.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     MK"
     gene            complement(17934..19676)
                     /gene="ggt"
                     /locus_tag="ACIE68_002171"
     CDS             complement(17934..19676)
                     /gene="ggt"
                     /locus_tag="ACIE68_002171"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFX5908482.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19796..20236
                     /gene="yhhA"
                     /locus_tag="ACIE68_002172"
     CDS             19796..20236
                     /gene="yhhA"
                     /locus_tag="ACIE68_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFX5908483.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(20223..20966)
                     /gene="ugpQ"
                     /locus_tag="ACIE68_002173"
     CDS             complement(20223..20966)
                     /gene="ugpQ"
                     /locus_tag="ACIE68_002173"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFX5908484.1"
                     /translation="MSNWPYPRIVAHRGCGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20963..22033)
                     /gene="ugpC"
                     /locus_tag="ACIE68_002174"
     CDS             complement(20963..22033)
                     /gene="ugpC"
                     /locus_tag="ACIE68_002174"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFX5908485.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            complement(22035..22880)
                     /gene="ugpE"
                     /locus_tag="ACIE68_002175"
     CDS             complement(22035..22880)
                     /gene="ugpE"
                     /locus_tag="ACIE68_002175"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFX5908486.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATASF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22877..23764)
                     /gene="ugpA"
                     /locus_tag="ACIE68_002176"
     CDS             complement(22877..23764)
                     /gene="ugpA"
                     /locus_tag="ACIE68_002176"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFX5908487.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23862..25178)
                     /gene="ugpB"
                     /locus_tag="ACIE68_002177"
     CDS             complement(23862..25178)
                     /gene="ugpB"
                     /locus_tag="ACIE68_002177"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFX5908488.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKNKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25574..26287)
                     /gene="livF"
                     /locus_tag="ACIE68_002178"
     CDS             complement(25574..26287)
                     /gene="livF"
                     /locus_tag="ACIE68_002178"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFX5908489.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(26289..27056)
                     /gene="livG"
                     /locus_tag="ACIE68_002179"
     CDS             complement(26289..27056)
                     /gene="livG"
                     /locus_tag="ACIE68_002179"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFX5908490.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(27053..28330)
                     /gene="livM"
                     /locus_tag="ACIE68_002180"
     CDS             complement(27053..28330)
                     /gene="livM"
                     /locus_tag="ACIE68_002180"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFX5908491.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(28327..29253)
                     /gene="livH"
                     /locus_tag="ACIE68_002181"
     CDS             complement(28327..29253)
                     /gene="livH"
                     /locus_tag="ACIE68_002181"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFX5908492.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTVFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(29301..30410)
                     /gene="livK"
                     /locus_tag="ACIE68_002182"
     CDS             complement(29301..30410)
                     /gene="livK"
                     /locus_tag="ACIE68_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFX5908493.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
BASE COUNT         7379 a         8545 c         7733 g         6956 t
ORIGIN      
        1 tttctctttt ttctggtgca ctatcgtccg cccacactcg ccccgagaaa aatagcatta
       61 atgcgcccag cgccagcata aaaatgttga gcagaaggta aattgttggc gtgataaaga
      121 tattacaaac ttcaataata ccggcaagtc cgacacccag catggcaata accgccgcca
      181 aaaactgcgc cagaatgggg atgccgaaaa aagtcattac cagcgaggtc aaaatccatt
      241 ttctgttttg cattattctt tccattcttt ctgaatggcg aaattatact ccccgagtcc
      301 ccttgcccct tctggacact tttccgaaat gatggcgaaa aaaacgggac cctttggccc
      361 cgttctattt attagtgaaa ttacaatctc accggatcaa tatgccagat atgatcggcg
      421 tactctttga tagtacggtc agaagagaag tagcccatat tggcaatgtt cagcatcgct
      481 tttgcggtcc actcttcctg aagctcgtag agttcgtcga ctttatcctg acaatcgaca
      541 tagctgcgat aatccgccag aacctgatag tgatcgccga agttgatcag cgaatcaacc
      601 agatcgcgat agcgacccgg atcttccgga ctgaatacac cgctgccgat ttgcgtcagc
      661 acctgatgca gctcctcatc tttctcgtag tattcacgcg gtttgtagcc ctgacgacgc
      721 agttcttcca cttcttccgc agtgttacca aagataaaga tattgtcagc accgacatga
      781 tccagcatct cgacattcgc accgtccaac gtaccgatag taagcgcacc gttaagcgca
      841 aacttcatgt tactggtgcc ggaagcttcc gtccccgcca gcgaaatctg ttcagacaga
      901 tcggccgccg gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat gaacacgact
      961 ttcagcttgt cgccaatctg cggatcgttg ttgatcactt tcgctacgtc attgatcaaa
     1021 tgaataatgt gcttcgccat gtaataggcc gaagccgcct taccgccaaa aatattcacg
     1081 cgcggtaccc acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt aatcacatgc
     1141 aacacattca tcaattgacg tttgtattcg tgaatacgtt tgatttgtac atcgaacaac
     1201 gcctttggat tcaccaccac attcagctgc tgggcgatat actctgccag acgctttttg
     1261 ttctccagct tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca gtgttgttgc
     1321 agctcattaa gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc gtccagcacg
     1381 gctgaaagcg atgggttcgc taccgctagc cagcgacgcg gcgtcacacc gttggtgacg
     1441 ttggtgaaac gacccgggaa gattttcgca aagtcggcaa acaacgattg caccatcaga
     1501 ttagagtgca gttccgatac accgttaact ttgtggctca caacaaccgc cagccaggcc
     1561 atacgcacac gacgaccgtt ggattcatca atgatcgacg cccgtcccag cagatcggta
     1621 tcgttcggat actgttcctg caaggttttc aggaaatagt cgttgatttc aaagatgatc
     1681 tgcaggtgac gcggcagaat tttacccagc atatcaaccg gccaggtttc cagcgcctcg
     1741 ctcatcagcg tgtggttagt gtaggagaag acctgacaac acacctcaaa cgcgtcgtcc
     1801 cagctaaatt ggtgctcatc gatcagcaga cgcatcatct caggaatcga cagtaccgga
     1861 tgggtatcat tgagatgaat cgcgatttta tccgccaggt tatcgtaggt tttatgcaac
     1921 tgataatggc ggcttaaaat gtcctgaatg gtcgaggaaa ccaggaagta ttcctgacgc
     1981 aggcgcagct cacgcccgga gtaggtggag tcatccggat acagtacgcg agatacgttc
     2041 tcggagtggt ttttatcttc cactgccgcg aagtagtcac cctggttgaa tttaccgagg
     2101 ttaatttcgc tactggcttg cgcactccac aaacgcaacg tgttggtcgc gtcggtgtcg
     2161 taaccaggga ttatctgatc gtaagcgact cccagaatct cttcggtttc aatccaacgc
     2221 gtttttttac cttcctgctg aatgcgacca ccaaaacgga ctttatagcg cgtgttgtgg
     2281 cgtttgaatt cccacgggtt accgtattcc agccagtagt ctggcgactc tttctggcta
     2341 ccgttaacga tgttctgctt gaacataccg tagtcatagc ggatgccgta accgcgcccc
     2401 ggcaacccta acgtcgccag agaatcaagg aagcaagccg ccagacgtcc caggccaccg
     2461 ttaccgaggc ctgggtcatt ttcttcatca atcagctctt cgagatttaa ccccatcgct
     2521 tccagtgcgc cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt ggagagcgta
     2581 cggccaatca aaaactccat cgacaggtag taaacctgac gagtttcttg cgacaactgg
     2641 gcacggtttg aacgtaacca gcgctccacg agacgatcgc gcacagcaaa taacgttgcg
     2701 ttcagccatt catgtttatt ggcgacgacc gggtcctttc caatcgtaaa catcagcttg
     2761 taagcgatag agtgcttaag agcttctacg ctaagcgtgg gcgatgaata tgtaaacgga
     2821 gcattcatat aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc acggtacgac
     2881 ttcgccgcga cctgccagct aaaatccatt gccatagcct gacgttgcac aaaccgccac
     2941 agtgaaggac gggaccacag tacaaaagca cgtcgaatag cccgtaacag cgaccaggca
     3001 ttactatctt caaagacaaa cccactggcg acgccatctg caaggttctc aagagaacag
     3061 tcagaaaccg tatcagcaag cccaccggtg cgccgcacta acggcagcgt accgtacttc
     3121 aatccataaa gttgcgttaa gccgcacggt tcaaaacggc tgggcaccag aatgacatcc
     3181 gcgccgccca taatgcgatg cgaaaatgct tcgtgatagc caatctgaac gcccacctgg
     3241 ccggggtatt ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc gcccgcgccg
     3301 agtagcgcca gctgcccgcc ctgctccaga aggcccggta aggcttccag caccaggtcg
     3361 agacctttct ggctggtcag acggctcacc actgcaaaaa gcggcacttt atcatcaacc
     3421 ttaagcccca ttgcgatttg taactggcgc ttattttccg ctttatcttc caacgtatcg
     3481 cgggtgtagc gcgaggccaa cagtaagtcc gtctctggac tccagatttt ctcgtccacg
     3541 ccgttcagta cgccggaaag acgcccttca cggtgacgct gttgcaacag accttccata
     3601 ccgtaggcaa actgcggttc ggtgatctcg cgagcgtagg ttggactgac cgccgtaatg
     3661 tgatcggcat agtacagacc ggccttcagg aaagagattt gtccgttgaa ttccagccca
     3721 tgaatattaa agaatgacca tggcaattgg atgtcattca tatgatgtgc ataaaacatg
     3781 ccttgatagg ccaggttgtg cacagtaaac accgacttcg ccggacgccc gcgcgccgcc
     3841 agatacgcag gcgcaaggcc tgcatgccag tcgtgcgcat gcaccacatc aggacgccag
     3901 aatgggtcaa gcccgctggc catttctgcc ccaacccacc ccagcagcgc aaaacgcaat
     3961 acgttgtcgg tataggcaaa taagttggta tcgtgatacg ggcttcccgg acgatcatag
     4021 agatgcggcg cgtcaatcag gtatatgcca accccgttgt aatgaccgaa caacagcgtg
     4081 atatgtccgg cgaaggtatc acgacgggat actacctgcg catcggtaac gccacggcga
     4141 atatcgggaa atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat ttgtgctgcg
     4201 ggtaatgccc caataacatc agccagaccg ccggttttaa gcagcgggaa catctctgaa
     4261 catacatgta aaacctgcat tatcgctcct gtttatgccc taacttccgt agcatttcgc
     4321 gcgttaccag cacgatgcct tcttctgaac gatagaaacg acgtgcatct tcctctgcgt
     4381 tttcaccaat caccatgcct tccggaataa cacaagcacg atcgatgacg cagcggcgca
     4441 gacggcacga gcgacctacc catacttccg gtaacaatac ggcggaatca atgttgcaga
     4501 atgaattcac gcgaacgcgc gagaacagaa cggactgcac caccaccgaa ccggagatca
     4561 cacagccgcc ggataccagt gagttaaggg tcatcccgtg gctaccggag cgatcctgca
     4621 cgaatttcgc tggcggtaat gattcattgt aggtgcgaat tggccaattg cgatcgtaca
     4681 tatccagttc cggcaccaca gaggccagat cgaggttcgc tttccagtaa gcttccagcg
     4741 tacccacatc gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa gagagcggga
     4801 acgggtgcgc ataggccaga ccggcttcgg tgatcttggg aatcaaatct ttgccaaagt
     4861 cgtggctgga gttctcatca cgatcgtctt cttccagcag ttcatacaga tagtcggcgt
     4921 caaagacgta gatacccata ctcgccagag atttgctcgg atcgttcggc attgacggtg
     4981 ggttagcagg tttttcaacg aattcgataa ttttatcgtt ctcatcaacc gccataacgc
     5041 caaatgcgga ggcttcttca atcggtactg gcatacaagc aacggtgcaa cgtgcgcctt
     5101 tttcgacgtg atcgataagc atacgcgagt agtcttgctt gtagatatgg tcgcccgcca
     5161 ggatcaccac gtattccgct ttataacggc ggataatgtc gaggttttgg gtgaccgcat
     5221 ctgcggtgcc gcgataccag ttttcccctt tcattctctg ctgtgctggc agcagatcga
     5281 caaactcgtt catttcttca ttgaagaatg accagccgcg ctgaatgtgc tgcaccagag
     5341 tgtgggactg gtactgggtg atcacgccca tacgacggat cccggagttg atgcagttag
     5401 acagcgcaaa gtcgataatg cggaacttac cgccgaagtg tacggccggt tttgctcgct
     5461 tattggttaa atccttcagg cgggtaccac gtcctcccgc cagtatcagg gcaacagatt
     5521 tcaatggcag ctggcgcgcc aacattaagt gatcgttctt ctctaaacta accatgacta
     5581 actccttttt tatcatctct ggaacacaca caatccgtgt gcaggtccct gccagacagc
     5641 cgtaatcact gggttatcct ctccagcgaa tgggggaatg gcgtgccact ccccagcagg
     5701 taaaacaatc tctgttacct caagcgtggc gttaattgcg atcaaaaagc gatccgagag
     5761 cagaatttgc agctgtttcg gcccgttttg ccactcatcc gtgcttaaag gttgagcata
     5821 tcgatttagc caacggacat tgccgtcgcc ttcttcccac cagcgattct ccaccaaagc
     5881 gggaatgcgc ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac cactgcttgc
     5941 ctgcgaccag tccaaccagg ttaattggtt atcctgacag taggcattgt tattgccatg
     6001 ctggctgtga ccatgttcgt caccggccag taacatcggc gtaccctggg agagcaacaa
     6061 cgttgttaac agggcgtgaa tgctgtcgcg ccgccgttca accaggtcaa gagaaccgcc
     6121 taacccttct ttaccatgat tgttactgta attgttgttg gtcccgtcgc gattttcttc
     6181 tccgtttgct tcattgtgtt tatggttgaa gcaaacgcag tcgcgaagcg taaaaccgtc
     6241 atgcgcggtg acgagattaa tcgcggcact cggcagacga ccattacgtt taaaaacatc
     6301 gctggaggca gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat gtagccagaa
     6361 acgacgggca gcatcgcgga aatgatcgtt ccactcggca aacagcggcg ggaaatttcc
     6421 cacctgataa ccaccaggag cgatatccca cggttcagca attaacttca cctgcgagag
     6481 caccgggcag ttctggatag cggtaaacaa cggcgcatcc tgacggaact ctggcgtacg
     6541 gcccatgact gccgccagat caaagcggaa accatcgacg tggcaggttt ctacccaata
     6601 acgcaggcag gcgctggcat aatccaccac cgccggatga ctcaaattga gcgtgttgcc
     6661 gcaaccggtc cagttgtgat aatcgccgtc ttctcttatc caataatagc tacggttatc
     6721 gatcccacgc agcgagaata acgggccgtc gaggtccagt tccgcactat ggttgagcac
     6781 gatatcaaga atgacttcga tacccgcttt atgcagtgct ttgattgcat cgcgaaactc
     6841 atccagcgcc gtttctggcg agcaggcata cgccggatgc agcgcaaaca tcgccaccgg
     6901 gttgtaaccc cagtagttac ttagccccat gcgttgcaga cgtggttcac tggcaaactg
     6961 cgccactggc agcagttcca gcgcggtaat gcccaattgt ttcaaatagt tgatcatcac
     7021 cggatgcccg agggctttat aagtgccacg gatctcgacc gggatctccg ggtgcaagta
     7081 cgttaatcct ttgacatggg cttcataaat gatggtgctg ccccacggcg tgcgcggcgg
     7141 ggcatcatct tcccagtcat agtgatcaac caccactacg catttcggcg caatggcggc
     7201 gttgtcgcga tagtcaggtt cattatgacc ggcgtgcagc agcgggttat ctttaaactc
     7261 cccctcaatt tgccgcgcgc aaggatcaat caacaacttc gccgggttaa agcgatgccc
     7321 ctcggcgggt tgccaggggc catgaacgcg ataaccataa cgcaaacccg ggcgcgcatc
     7381 cggcagataa ccgtgccaaa tgtcgccact gtgccctggc aagtcatagc gatgttcctg
     7441 gccattggcg tcaaagacac acagttctac ccgctcggca tgagcggaga aaagtgtgaa
     7501 gttgacgccc tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa tggcgagttg
     7561 tgtcattctg cctcccgaac cagccagata gtggccagcg gtggtagcgt caggcttagt
     7621 gaatgctgac gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc attgcctgca
     7681 ttactgccgt gatagtgcat ggaatcggta ttgaggattt cacgccattt gcccggctgg
     7741 tttatgccga agcgataatc atgacgcggc accggcgtaa agttactggc aacgatgatt
     7801 tcgttaccct ctttatcgcg acgcacaaag atcagcaccg agcgttcttt gtcatccacc
     7861 accagccatt caaagccgta cgggtcaaaa tccagttcat gcattgcttt atggtggcgg
     7921 taggtgaggt tcagatcgcg caccagacgc tggacaccgt ggtgccagtt atcgccgcct
     7981 tccaacagat gccagtcgag gctggcgtca tggttccact cgcggccctg ggcaaattcg
     8041 ttacccatga acagtagttt cttgcccggg aatgcccaca tccagccata gtaggcgcgc
     8101 aggttcgcga atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga ttttttaccg
     8161 tggaccactt catcatgcga caacggcagg acgaagtttt cagtgtagtt gtagagaatc
     8221 ccgaaggtca gtttatcgtg atgatactga cgataaaccg ggtcgagctt catgtagtcc
     8281 agggtgtcat gcatccagcc gaggttccac ttgtaccaga agcccagacc gcccatatcc
     8341 tgcggacgag aaacgccagg gaaatcggta gactcctcag ccattgtcac cgcaccggaa
     8401 acctgctcac caagaatacg gttggtatta cgcaagaatt caatcgcttc aagattctcg
     8461 cgcccgccaa attcgttcgg gatccactcc ccctctttac ggctgtagtc gcgataaatc
     8521 attgacgcca ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat ccagtaaagc
     8581 gcgttaccga cgaggaagtt actgacttca cggcgaccat agttgtagat cagcgtgttc
     8641 cagtcctgat gatagccttc acgcggatcg ctgtgttcat acaagttcgt gccatcaaat
     8701 tcggcaagcg caaagtcatc agtcgggaag tggcctggca cccagtcgag aatcacgttc
     8761 agaccagctg cgtgtgcggc atcaatgaaa taacggaagt cgtcgcgagt accaaaacgg
     8821 cgggttggtg catacaggcc ggttggctga taaccccaac tgccatcgaa gggatgctcg
     8881 ttaatgggca gtagttcgag gtgggtaaag cccatccatt tagcataagg caccagttga
     8941 tcggccagct cgcggtagct caaccagaaa ttgttgtcgg tgtggcgacg ccaggaaccc
     9001 aggtgaactt cataaataga gattggcgca tcaaactgat tcgctttttt gcgctcttca
     9061 gtctgtacaa ccttttccgg cagcccgcaa ataagagacg cggtttccgg gcgcatttgc
     9121 gcttcgaagg cataagggtc ggacttcaga cgcaagttgc cattggcatc aatcatctcg
     9181 tatttataga gctgaccgtt atgcgcccca gggataaaca gttcccagat gccgctctct
     9241 ttacgcaggc gcatcgggtg acggcgaccg tcccagtagt tgaattgccc aaccaccgag
     9301 acccgacggg cgtttggagc ccagacagag aaacgcgtac ctgtgacgcc atccatagta
     9361 tctgcatgcg cgcctaaggt ttcatacggg cgcaggtgag taccttcaga taatagccag
     9421 gcatccattt cctggattag cggaccaaaa cggtaaggat catcaatcag gttttgctga
     9481 ccatgccaga caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg aatgacgccg
     9541 ctaaagaatc cccgtgagtc gagacactcc agttttgcga gtttgcgccc ggttttcggt
     9601 tcaatcaccc acacatcggt agcgtcgggt aaaagggcac ggacttccag tcccgcggtg
     9661 gttttatgca ttcccagtac ggaaaaagga tccgcaaaat ggcctgcaat tagcgcgtta
     9721 atcacgtctc tatcgatacg atcggacatg cttgtcttcc tgttttattg tgtcacccca
     9781 tccaattgga tttttgactt ctggttgtga catttttttg acctgaacgg cgcagcactc
     9841 tgtgcatcct ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc atgtattcag
     9901 ccacccttaa agaatagcca atgctctatt taactcccgg taaatcatga aacatctgcg
     9961 cttactcctg tattacgcac taacaggggc ggcatcgcgc cccagattta atgaataaag
    10021 attacgccag ttgacgaagc atccgacgca gcggctccgc ggccccccac agcagctggt
    10081 cgcccacggt aaaggctgac aggaactctg gtcccatatt cagcttacgc aggcggccta
    10141 ccggcgtggt cagcgtgccg gtaacggcag ctggggttag ctcacgcata gtgatttccc
    10201 gatcgttcgg aacgactttc gcccacggat tgtgcgcagc cagcagttct tccacggtcg
    10261 gaatagacac atcttttttc aatttaatag tgaatgcctg gctgtggcag cgcaatgccc
    10321 cgacacgcac acataaacca tctaccggaa ttacggaaga tgtgttgagg atcttgttgg
    10381 tttccgcctg ccctttccac tcttcgcggc tctgaccgtt atcgagctgt ttgtcgatcc
    10441 acggaatcag gctacccgcc agcggcacgc caaagttatc caccggcagc tcaccgctac
    10501 gggttaaggt tgtgactttg cgttcgatat cgagaatagc agaggacggg gtcgcgagtt
    10561 catctgccac atggccatac agatggccca tctgggttaa taactcacgc atatgtcgcg
    10621 caccaccgcc ggaagcggcc tggtaggttg caacggacac ccaatcaaca agatcattgg
    10681 cgaataaacc acccaacgac atcaacatca ggcttacggt acagttaccg ccaacaaaag
    10741 tcctgatgcc attatttaat ccgtcggtaa tgacgtcctg attgacgggg tcaagaatga
    10801 tgatggcgtc atctttcatg cgcagagacg atgctgcgtc aatccagtaa ccttgccatc
    10861 cgctttcacg aagctttgga tagatttcgt tggtataatc gccgccctga caggtcacaa
    10921 tgatatcgag ggcctttagc gcctccagat caaaggcatc ctgaagtgtg ccagtggttc
    10981 cgccaaaaga cggcgcagcc tggccaagct gagaagtaga aaagaagaca gggcgaatgg
    11041 cgtcgaagtc gcgctcttca accatgcgtt gcatgagaac ggagccgacc ataccgcgcc
    11101 agccgataaa accaacattt ttcataagcg tttttttcct gcaaagatgt gtgctgtata
    11161 aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca aagtcgcaag
    11221 tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct ttgttagcac
    11281 gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca tttctgcagc
    11341 agttttattg atcctgatta tggatccgct cggaaaccta cctattttca tgtccgtact
    11401 gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc ttattgctct
    11461 cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc tcagcctgcg
    11521 agcagaaacc gtctccattt ctggcggcat cattctgttt ctaattgcca ttaagatgat
    11581 tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc catttatcgt
    11641 gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga tgttgttgtc
    11701 tcatcagtac ccgaatcaga tggggcatct ggtgattgct ctgctgctgg cctggggcgg
    11761 cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg agaaaggggt
    11821 gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaaccc agatgttcct
    11881 cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa agaacccggt
    11941 agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa tcatcccaac
    12001 gatggcaccg acagtgccga ggatggtttc catcatggtc caggttttca gcgtttcggc
    12061 ttcggtcgcg ccggtaaatt taccgaacaa ccagaaaccg gcgtcgttaa cgtggctgac
    12121 aacaatcgaa ccaccagcga tacaaatcga cagcgccgcc atttgcgcac cggagtagtt
    12181 cagttgttca ataaccggca tcaccagtcc taccgccgtt aaacaggcta cggtggcaga
    12241 accctgaatg atgcgcactg cagctgccag cacgaagcag gtgatagcaa tcggcaggcc
    12301 catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca gcacctgttt
    12361 gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca gcgcgtgacc
    12421 gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc cataaatcgc
    12481 taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga accattcgta
    12541 agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca ccagcaccag
    12601 cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttgcctt cgccgagatg
    12661 cggttcgctg atgtcgtcag gaatatgcaa ctccacgtag cggctgatga aattacccca
    12721 cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca ggatcatcca
    12781 gccaaaatcg gcgttcatct gcgatgccag cagcattggc gctggccctg gcaccaggaa
    12841 cgccgccgct gccgccacgc ctgcgaataa tgggattacc agcttcacca ggttcgtacc
    12901 ggtgtggcgc gccattgaga aagcaacgct aatcagcaga actatcgcca cttcaaagaa
    12961 cagcggtagc gcacagacca accccgcaag gccgatggca aaatgcgcgc ggctgtgacc
    13021 gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat gtaagatctt
    13081 gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc ctcccatccc
    13141 tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc cagcccccat
    13201 ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta aaaacagcag
    13261 cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta cttatttgcc
    13321 ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat cgatatccac
    13381 caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact gcgtcaccaa
    13441 catttgggtt ttaaagaaat ggcctttgcg cgctttcaag cgactttcaa tcacatcaaa
    13501 atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca agtcgcgata
    13561 gtgctttttc agtgcagaac agacgatcag cgacacttta ttggtgcgct gcatagcaaa
    13621 cgcggcgtcg ttcagcgcct gcagccacgg tttgcgatcg tcatcattca gcggttcgcc
    13681 agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa gaaacgcggc
    13741 atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg atacgcccat
    13801 caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc cacaatacaa
    13861 gaattaaaaa tgttacgggt aactgttatc ggtaacattg tccagccgga caatatcaga
    13921 agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt tgtgggctta
    13981 aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg tcacagattc
    14041 gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct cacgcggcgt
    14101 cagcacgctc gccagtcgtg gctccatcac ctgaccaatg tcatgaccgt ggaaaccggc
    14161 aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg ccgcgccgac
    14221 cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc gccgcgcctg
    14281 gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca cgctatacgg
    14341 caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga tagtacgttc
    14401 gtcgagacgt gcgccgagat aggcaatgtg gcgatgcccg cgcgcaataa tggcggtagt
    14461 catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc acggcgactg
    14521 gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta aggtgcgcgg
    14581 cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggacagca tggattcgag
    14641 acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatcgtct gataaccgtg
    14701 cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt tggtgagaga
    14761 aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg gcgcacgatt
    14821 gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag cgacggaaac
    14881 ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta cacggtcagc
    14941 cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa agagatagat
    15001 tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc ttgtttacaa
    15061 ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg caaaaccccg
    15121 gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag gcaggcttca
    15181 aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg ctgtcgctat
    15241 ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg gtcgaggctt
    15301 tcacgccgtt aatggtgaca ttgcctttca ccacctggat ccagacgcgg cgttcagcgg
    15361 caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt tccatatcct
    15421 gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt ttgccctgca
    15481 cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca ggcatgatcc
    15541 agatctgata cagatgcaga cgctcggtgc tgtttgggtt gtactctgag tgacgaatac
    15601 ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta ttgcccatgc
    15661 tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata tctttatgcg
    15721 ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg cgcagcgcgg
    15781 agaagcccat aaagttcgga tcgtaatagt tggcaaaaga gaaagtatgc caggagtcca
    15841 gccagccatg atttgcatga ccacgttcat ttgctttgcg gaagtagatc atttgtattc
    15901 gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga tgatagaggg
    15961 tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc aaacttactt
    16021 agcgagagtt actgtggagg gagaggcttg ctcaaatccg cgttcaagga tttccagatt
    16081 ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg tttgatacaa
    16141 cgcatcataa acgcgcccgt aatcgcccat ctccggcttc atctcttctc tgaccgtcac
    16201 gccctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga atcccggttc
    16261 gcccggcata ataatagcct tcaggctggt ttcctgctgg tcgataccgt atttaataaa
    16321 cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca gatggctggt
    16381 tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt cgtcaggatt
    16441 ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac cgaacagaga
    16501 aataatctgg tccatcgtat gcacaccaag gccataaaac gcgccatcct gcggcagccc
    16561 aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt caacaatctc
    16621 tcccaacttg ccactttcaa tcgctttttt cgctgtcagg aagcaggagt caaagcgacg
    16681 attctgatac ggcgtgacgg tcagcccttt gcttttcgcc aacgcaaaca gctccttcgc
    16741 ctgggcaagc gtcggggtga acggtttttc gaccagcaca tttttcccgg cttccagcgc
    16801 gcgtttcgcg tactcgaaat ggctgtccgc gtgggtgcag acaacaacca gcttaacatc
    16861 gggatcgttt agtacttcgt cgagatcgct ggtcaaatgg atatgggaat aaatgggagc
    16921 ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc tatccttgcg
    16981 gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa taaaggcgca
    17041 gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc gcaaagcgga
    17101 cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata aattgagaac
    17161 gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag caatgtgagc
    17221 aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgagacaa taataatcat
    17281 tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg catttgaaag
    17341 tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt gagatagtaa
    17401 tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc cagccggagg
    17461 tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag gagcgactcg
    17521 ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc gtgggccatc
    17581 tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt ggtatctgtg
    17641 tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag atgattgaaa
    17701 tgtgcgacaa ctggttgcgg gtagatcgta tcgagctaac ggtgttcgtc gataacgcgc
    17761 cagcaattaa ggtctataaa aaattcggct ttgagattga agggactggc aagaagtacg
    17821 cattacgtaa tggtgaatat gtcgatgcgt attatatggc gcggatgaaa tagagaattt
    17881 gcaatctaag tttgtcattt aagccctcac ctaccgggag agggcttaag atattagtaa
    17941 cccgccgtta aatcatccac cgagcgcggg tcggatgcgc cgtacaactc accgtccggc
    18001 ccaaccataa tgctttgtgt actgcccatc gcctctttca gcgccacttt ctgacctttt
    18061 gcttccagca gcttgagcgt atccgggcta aacccttttt cgacacgcag ctcgtccggc
    18121 aaccactgat ggtggaaacg cggcgcattg gtcgcttcgg cgacgttcat gccataatcg
    18181 atgctattca ccaccatttg cagcactgta gtgatgatcc ggctaccgcc tgggctaccg
    18241 gtaaccagcc aggttttacc gtctttcacc acaatggtcg gcgacatcga cgacagcggg
    18301 cgtttgttcg gcccgacggc gttggcatca ccgcccacca gcccgtaaac gttcggtacg
    18361 cccggtttgg cggagaaatc atccatctgg ttattaagca gaataccgct ctcgcccgcg
    18421 acaatacccg taccgaaggt ggtgttcagc gtataggtca ccgccaccgc gttaccatct
    18481 ttatccacca ctgagtaatg ggtagtttga ttactctcat aaggcgcaag cttgccgggg
    18541 cgaatttcgc tggatggctt cgctttattg atatcaattt gatcggcaat agatttggca
    18601 taggctttat tggtcagcgc ctgccacggt actttgacaa aatccgggtc gccaagatat
    18661 tccgagcggt cggcgtaggc gtatttctcc gcttctgcca tgatttgcat cgcatcggcg
    18721 ctgccaaagc cgtatttctt catatcgaag ttttccagaa tattgaggat ttgtacgata
    18781 tggatcccgc cggaggatgg cggtggcatg gagtaaacct gatacccgcg ataatcgccg
    18841 cttatcggag tgcgttcgac cgctttatag gccgctaaat cttctttagt gatcaagcca
    18901 ccgtttttct gcatctcctg ggcgatctgt tccgcaatcg tgcctttata gaattcgtcc
    18961 gggccgtttt cagcaatcat ctccaggctc tttgccaggt tcgcctgcac cagcgtgtcg
    19021 ccctttttca gcggctcgcc ctctttccag aagatagctt tactgttttc gtgattcggc
    19081 aacacttcgc taccgtaggt tttgagatcg tcagccagtg cgtcgttaac gataaaacca
    19141 tcgcgtgcca gtttaaacgc gggctgcacg actttgttca gcggcatggt gccgtattta
    19201 tccagcgcca gcgagaaacc tgctaccgta cccggtgtgc cggaagccag atgcgaagtg
    19261 agtgattttt tgctgtccgg gttgccctga tcatcgagga acatatcgcg ggtcgctttg
    19321 gcgggtgcca tttcgcggaa atcgatagcc gtggtattgc catttttcga gcggattaac
    19381 ataaaaccac caccgcccag attccctgcc tgcggatgcg ttaccgccag cgcgtagccc
    19441 accgccacgg cggcatcaac ggcattcccg ccctccttga ggatatccac ccccacctga
    19501 gtggcagtgg cgtccacaga cgctaccatt ccctgtttcg cgcgtaccgg gtggaagaca
    19561 tcttcctcca caccatacga gacgggcggc gcaggaggcg cggcggcggc gctaaaacaa
    19621 cttcctgaga gcagagcagc aatggccacc cggcgtaaaa acgtcggttt tatcatcgtt
    19681 attctccaga gattaagggg caaccccagc taagcctggt atataactct gaattaatca
    19741 tcgttttgtc gggaagcgag taaacttaaa ggatatcttc agaggaggat acgcgatgaa
    19801 acgacttctg attcttacgg cactcctgcc gtttgtcggc tttgcacagc ccattaatac
    19861 tctgaacaac cctaaccagc cggggtatca gatacccagc cagcagcgga tgcaaaccca
    19921 gatgcagact cagcaaatcc agcaaaaagg gatgctgaat cagcaactga aaacgcaaac
    19981 tcagttgcaa cagcagcatt tagaaaacca gataaacaat aattctcagc gggtgttgca
    20041 gtcacagccg ggggagcgaa atcccgcccg gcagcaaatg ctgcccaaca ccaacggcgg
    20101 gatgttaaac agcaaccgta atccggatag ttcgttgaat cagcagcata tgctgccgga
    20161 gaggagaaac ggcgacatgc tgaatcagcc cagcacgccg cagcctgaca ttccgttgaa
    20221 aactattggg ccgtaaagtt cggaccaatc acgtcaatcg catcggtaca gatgcaatcc
    20281 acgccccagc gcagcaactc tgccgcgcgc tggggtttat tgacggtata aaccagaatc
    20341 cgcagtccgg cgtctttcaa ctgcatcact cgcactttat cgagtaactt atgattgaga
    20401 tgaatagaga cgcagcccag ccgcgcggtc agttcgcgcc agtcgtcgcg ccactcatca
    20461 agcaataaac cacgcggcag ttccggtgcc gccagctgtg cagcttctaa agcatcaatc
    20521 tcaaacgatg acagcagcgg cggcgtcata ccggcccaca gttggcgtgc cgccagcgcc
    20581 accattttgc ccgttaatgg tccagtgcca gtggtgggtt tgatttcgat attcgccatc
    20641 atcccgtgtt cgcgacagcg ttccgccacc tgcgaaagca acggcagcgg ctcaccttta
    20701 aacgctttgc tgtaccaact gcccgcatcc acgcgcagta aatcctgcca gttcagttca
    20761 cccgcgacgc cccagccgtt gctggtgcgt tcgagattgt cgtcatggag caggaagatc
    20821 tcgccatctt tcgataactt cgcgtcaaat tcgatcatct tatgaccgta ttttgccccg
    20881 acgtcgattg ccgccagggt gttttccggg gccagcttac cgcagccacg atgagcgacg
    20941 atgcggggat aaggccagtt actcatactc gttgtcctgt ttcaccatca aaaagatgca
    21001 gctgattttc cgccagatgc agccacagcg tgctgcctgc cgtcgggcgc tcctgatgcg
    21061 ccagtcgcac caccagcttc tgctcgcccc agcgtccgtg cgccaggtta tctgcgccga
    21121 ggatctccag cgtgtccatc accagcggta cgccgccttc tgcctgcgag cttagcgcaa
    21181 tatgttccgg gcgaatgccg agagtcattt tacgcccggc atactgacgg tagccaccgt
    21241 ttagcggcag ctcaataccg ccgtccagtt cgaaatgcgt gccttcgtta ttcacgcggc
    21301 ctgtcagcag gttcatcgcc gggctgccga taaaactcgc tacaaacagg ctggcgggct
    21361 tttcgtagac ttcaactggt gtgccaatct gttcggcaac gccgccgttc atcaccatta
    21421 ctcgctgggc gagcgtcatc gcttcaacct gatcgtgagt aacgtagagt gaagtcgttt
    21481 tcaggcgacg gtgcaactgt tgcagttcaa gacgcatctg cacgcgcagc ttggcatcga
    21541 ggttagagag cggctcatca aacaggaaca ccgccggatc gcgcacaatc gcgcggccca
    21601 tcgccacacg ctggcgctga ccgccggaaa gctcgcgcgg gcgacgtttg agcagaccgt
    21661 ccagctccag aatgcgcgcc gcttctttaa cgcgctcggc aatttgctgc ttgcccatgc
    21721 cgcgaatttt cagcccccac gccatgtttt cttcgacact catatgcgga taaagcgcgt
    21781 agttctggaa caccatcgca atcccgcgat cttttggctc catttcagtc acgcgctggt
    21841 cgttgatcca gatatcgcct tctgtcaccc gctccagccc ggcaaccatg cgcagcagcg
    21901 tcgatttccc gcagccagac ggcccgacca tcacgataaa ttcgccatcc gccacatcaa
    21961 gggtcagcgg tttaatgacc tgggttttac catcccagct tttggttact gcctgtaatt
    22021 tcagtcctgc catcttattt ctcactatcg accaggccgc gcacgaaggc acgctgcatc
    22081 actaaaacaa tcaccaccgg agggataagc gttaacaaca tcgccgccat cactgagttc
    22141 cattcagtgg tgccttcgcc tgtagcgatc atccctttga tccctgccac ggtggtgccg
    22201 agatccacat cggtaataat caacaacggc cacaaatact ggttccagcc gtaaataaag
    22261 gtgatcacaa acagcgccgc cagattggtt ttggagagcg gaaaaacgat gtcgcaaaag
    22321 aaacgcattg gtgacgcgcc gtcgatccgc gccgcttcca ccagttcatc cggcagcgtc
    22381 ataaagaact ggcgaaacag aaacgaggcg gtcgccgagg ccatcagcgg cagcgttaaa
    22441 ccggcgtagc tgtcgagcat cttcaggttg gcgatgactt ccaccgtcgg gaagatacgt
    22501 acttcaaccg gcagcatcag ggtgataaaa atcatccaga agaagaggtt acgtagcgga
    22561 aaacgaaacc agacaatggc aaatgccgag agcatcgaga cggtaatttt gccgagcgta
    22621 atgctgaacg ccatcacaaa gctgttaagc aacatccgcc agaacggcgc gctattcgtg
    22681 cctaccccgt tcacccagat gttgtggatg ttttccagca gatgtgtgcc ggggatgagc
    22741 gtcatcggcg cggcatagac ggcctgttta tccagcgtcg ccgcgacaaa cgccacgtac
    22801 agcgggaaga ggatcaccgc gatcccgagg atcagcatgg tatggctgaa tatcgtcagc
    22861 cacggacggt tctcaatcat tggtaacgca ccttgctttc aacatagcgg aactgcacca
    22921 ccgtcagcac gatgacgagg aacatcaaca ccaccgactg tgcggcagac gaagccagat
    22981 ccagtccggt aaaaccttcg cggtagatct tataaatcag cgtcgtggtg gcctgaaccg
    23041 gcccgccgga cgtggcggcg tcgatcaccg ggaaggtgtc gaagaaggca tacaccaggt
    23101 tcactaccag caggaagaaa ctcaccgggg cgataagcgg cagcgcaatc ttaaagaagc
    23161 ggcgaatcgg ccctgcaccg tcgatggctg cggcttcgat caacgaacgg ggaatggatt
    23221 gcagcgcggc atagaagaac aggaagttgt agctgatttg cttccatact gaggcaaaca
    23281 ccaccagaaa cattgcctga ccgctgtttt gcgcgtggtt ccagtcgtag ccgaactccg
    23341 cgagaaaatg ggtgatcagc ccgcgaccgg ggttaaacag gaagatccac aatacggcgg
    23401 caacggcggg agccacggcg taaggcagca acattaaggt ttgatagaaa cggctgccgc
    23461 gcacgatgta ctccaccagc gccgcgaaga acagcgacac cagcaaaccg ctgacggtga
    23521 caaaggtgct gaattttatc gtcgtccaga aggagtcgag atagtagctg tcatgaaaca
    23581 gcgcgacgaa gttatccagg ccgacaaact ggctggagaa accaaacgga tcgacgcttt
    23641 gtagcgagta ccacaacgct tcgcccgcag gccagataaa aaagataacg gtgatgatga
    23701 gctgcggcgc gaccagcaga taaggcagcc agcgcgagcg gaacaccgga cgggatgatg
    23761 acattacggt taattcctga actgtgccgg atgcgcttcg cttatccggc ctacacgcga
    23821 tgccaggtca gataagcgaa acgcatccga cattacactg attaagactt cgtcgatttc
    23881 tcaaagcggc gcagcaactg atttccacgc tcaacggcgg tatccagtgc ctgctgtggt
    23941 gtcttcttac cggtccacac gctctccagc tcttcatcca caatcacgcg gatctgcggc
    24001 atgttgccca gacgcagccc tttggtgaac ggcaacggcg gcttgttcag catctgacgc
    24061 gtcgcggtat ccgcccctgg gtttttctca taaaagccct gctcacgggt caggtcatac
    24121 gctgctttgg tgattggcag ataaccggtt ttctgatgcc actcggcagc gttttctggc
    24181 ttcgcgagga agtcgaggaa cttcgccacg ccggtatacg tttctttatt tttgccctgc
    24241 atcacccaca ggctggcccc gccgataatg gcgttttgcg gtgcatcttt cgcatcggcg
    24301 tcgtaaggca tcatgcctac gccatagtta aatttggcgt actcacgaat attagcgaga
    24361 gaaccggaag aggcggtggt catcgcgcaa tcaccgttat agaacttctc ggtggattca
    24421 tccttacgac cgacgtagct gaagtcgccc ttcttgttca tctcctcgag catggcgatg
    24481 tgtttcacct gctccggctt gttgaactcc agcaccgcgt ccgtgccgtc aaagccgttg
    24541 tttttgctgg caaacggcag accgttccag gcgctaaagt tttccagttg gatccagccc
    24601 tgccagccgc tggcgtagcc gcacttcata ccggaggctt tcagtttcgc ggcatagtcc
    24661 gccagatcct gccaggtttt cggcggttgt tccgggtcta atcctgcttt cttgaaggcg
    24721 tctttgttgt aatagagaac gggggtcgag ctgttgaatg gctgggagag taagtggccc
    24781 gttttgctgt cggagtagta accggaaacc gtcggcacaa actgcgactc atcgaattga
    24841 atccccgcct ctttaaacac gtcatatacc ggtttaatgg ctttcgatgc catcatggtg
    24901 gcggtgccaa cttcataaac ctgcaaaata gccggagcgt tgccggtacg aaatgcggca
    24961 atccccgcgc ttaaattctg ttcgtagttg cctttatagg tcggtacaat tttgtaatcc
    25021 ggattttcgg cgttaaaacg ttgggccaga gaatccacct ctttacccag ttccccttcc
    25081 atagaatgcc agaacggaat ggtcgtcact gcctgtgcat tccccattaa cgccagtccg
    25141 agcgccagtg ctgaagctgt ataatgtaac ggtttcatcg tttatctctc ttgttgtacc
    25201 gaatgcgcga attcacgcgt tttatgctcg cggggtaaca tgacatgctc gaattacaga
    25261 aaaataactt ttttgttaca tttgtaagat agtaaggtgt cagaaagatg acaaggcggt
    25321 gacggcgtgg gtgagggaaa atgggagatg gggcacggat aagcggaaaa tagaaggtct
    25381 gaatcaaact ctacagatta ctcatcgttt catgccggat gcggcgtaaa cgccttatcc
    25441 ggcctacaag atcgtgcaaa ttcaatatat tgcaactcac ccagtaggcc tgataagcgc
    25501 agcgcatcag gcaattttac atttgtcacc tgtctcaaag gagtcttttg actccttatc
    25561 aatcaacgtg ttattacccg cctaaatacg cacttctcac tgcttcgttc gccagcagcg
    25621 cgtcgccggt atcagagagc accacatggc cgttttccag cacgtagccg cgatccgcca
    25681 gctttagcgc ctggttggcg ttctgctcga cgaggaagat agtcatcccc tgctcgcgca
    25741 gctgctcgat ggtgtcgaaa atttgctgga tgataatcgg cgcaagaccg agcgatggct
    25801 catcaagtag cagcaaacgc gggttgctca tcagcgcgcg accaatcgcc agcatttgtt
    25861 gctcaccgcc ggacatggtg cctgcccgct gaacacggcg ctcatgcaga cgtggaaaca
    25921 gttcatacac ccactttatg cgctcctgga actggtcgcg ttcggcaaaa aagccgccca
    25981 tcgccaggtt ctcttccacc gtcatccgcg agaagacgcg acgcccttcc gggacaatcg
    26041 ctaccgcttc gcgcatgatt ttcgccgtct gccagtcggt aatgtcttta tcatcaaaca
    26101 caattcgccc gctggtagcg cgcggatcgc cgcataacgt gccgagcaat gtggttttcc
    26161 ccgccccgtt cgcgccaatg agggtgacaa tctcgccctg attgatatgc aggctcacct
    26221 catgcagcgc ctggattttg ccgtagtggg cgctgacttt gtcaaaggac aacatgactt
    26281 tttccatctt atgcctcacc taaataggca cggatcacgt ccgggttatt acggatttgc
    26341 tccggcgtac cgtttgccag tggcgtcccc tgattgacca cataaattcg gtctgaaatc
    26401 cccatcacca gcttcatatc gtgttcaatc aacaagatag tggtgttgtg atgattgcgc
    26461 agttcggcaa tcagctcatc cagctctttg gtttcttttg ggttaagacc tgccgccggt
    26521 tcgtcgagca ttaaaatctc cggctgcgtc accatgcagc gggcaatctc cagacggcgt
    26581 tggtcaccat aggccaggtt actcgcctga cggttggcgt gttccagcaa accaatgcgc
    26641 tcaagccagg tggcggcgcg gtcgagcgct tcgctctggg cgcgacggaa agaaggcgtt
    26701 ttcaacaggc cagagaacag cccggttttc agttgttgat gctgcgccac cagcaggttt
    26761 tcaatcaccg tcatttcacg gaacagacgc acatgctgga aggtacgcac cacgcccatg
    26821 cgggcaattt gctgccccgg taaaccttcc agatgctgat cacgcagtaa aatagtgccg
    26881 ccggtgggtt tgtagaatcc ggtcaggcag ttaaaaaccg tggtttttcc ggcaccgtta
    26941 gggccgatta acgagacgat ctcctgcggg tacagttcaa gattgacgtt gttcaccgcc
    27001 agcaagccgc cgaagcgcat catcagaccg ttaacagata ataatggctg actcatgcct
    27061 gctctccttt cgctgcgccg tttttcagct tcagttgcgg gcgcgtcatg ggcagcaagc
    27121 cctgcggacg ccagatcatc atcagcacca tcaaaccacc gagcattaac atgctgtatt
    27181 cgttgaaatc acgcatcaat tcgcgcgata ccaccagcaa aattgccgcc agaatcaccg
    27241 caaactgcga tcccataccg ccgagcacca ctatcgccag tacaaacgcc gattcagcaa
    27301 aggtgaagga ttccgggctg acaaagccct ggcgcgcagc aaacagcgtt ccggcaaaac
    27361 cggcaaacgc ggcacttatg gtaaaggcag tcagcttgat acgacgcggg cttaagccca
    27421 gcgaacggca ggcgatctcg tcttcacgca acgcttccca cgcacgcccc agcggcatcc
    27481 gcagcaggcg gttaatgaca aacaggctta gcaccaccag cagcaacgcc accaggtaga
    27541 ggaagatgac acgatcggag ggatcgtatt tcaggccaaa gaaattactg aacgtgtccc
    27601 agccgccttc acgagcggta cggctgaact cgagtccgaa gagtgtcggt ttcgggatct
    27661 gactgattcc gttcgggccg ccggtaattt cggtgttatt gagcagcaat atgcgcacaa
    27721 tttcgccgaa accgagggta acgatcgcca gatagtcgcc gcgcaagcga agcaccggga
    27781 agccgagcag gaagccggcc gccgctgcca ttaacccggc aatcggcaga caggtccaga
    27841 agcccaggcc gtaatagtga ttgagcagcg caaaagtgta agcgccgatg gcgtaaaagc
    27901 cgccgtaccc cagcaccagc agaccagaaa gaccaacaac cacgttcagc ccgaggccga
    27961 ggataatgta gatcatggtt agggtggcga tatccaccgt cccacgcgac accataaacg
    28021 gccacgccac cgcaagcacc agcagcgcca cgaggaatag tttctgcttc accgtggagc
    28081 catcaatcgc gggcagaata aacttcggtc cggaaacgct ttttagccct ttctggaaag
    28141 ccggtcgcaa aagctggaag aaaaagacca ccgccgtgcc gataaacacc cactgccaac
    28201 ggatgtctga agccgtgtcg accaccagtt tggtgccatc cagctccagt tgcacgccca
    28261 taaagacgcc cgccaggaca aaaaacatcg cggcagagag cagcgccatt gcaatatgca
    28321 tcggtttcat actttctcta cctccgggcg acccagaata ccggtcggca tcaccagcag
    28381 caccagaatc agcagggcga atgagaccac atctttatat tccgtactca gataggcaga
    28441 agagagcgcc tccgcaatcc ccagaatcag cccgccaatc atcgctcccg gaatgctgcc
    28501 aatcccaccg agcaccgcag cggtaaaggc tttcatcccg gccataaagc cgatgtaggg
    28561 gttaattacg ccgtagaact gaccgagcag cacacctgcc accgccgcca tcgccgcgcc
    28621 aatcacaaag gtcagcgcaa tcacccggtc ggtgttaatg ccaagcaggc tcgccatttt
    28681 caggtcttcc gcgcaggcac gacacgcgcg ccccatacgg gaatagcgaa tgaaaaccgt
    28741 cagcgccagc atggcgagga aggtgacaat ccagatcacc gcctgcatgg tggtaataga
    28801 ggcagagaag ttttcgctat gccccaccac ccactgaccg ttaaacagac tgggcagcgc
    28861 cacgtcacgc gaaccttcag tcaggctaac gtagttttgc aggaagatgg acatgccgat
    28921 tgccgagatg agcgcaatca ggcgcttaga gttacgcacc gggcgatagg ctacccgttc
    28981 gatactccag ccgtaggcgc tggcgatgac gattgcgccg acgaatcccg ccgccaccag
    29041 cagccagccg gtatcaatgc ccatcatcat cagcgcggcg atgatcataa atgagacata
    29101 gctgccgatc atataaacct cgccgtgggc gaagttgatc atgccgataa tgccgtaaac
    29161 catggtgtag ccgatggcaa tcagcgcgta ggtactgccc agcgtgacgc cgttaaacat
    29221 ctgctgcaag aaatataaaa actgctcaga cataaggtaa cctttctaaa cccgcccgtt
    29281 tttcacgggc ggtgggatga tcacttggct gccgtggatg aaccgtcggc gtgccactgg
    29341 aagacaccaa aatcaaatcc cttaagatcg cctttttcat cccagttcag cggcccaatc
    29401 acggtgtttg caccgttagc ttttaaatct ttcaccagcg ccagcggctc atcgctgcca
    29461 gtacgctcaa gggcagtcgc cagagattgc accgccgcgt aggtgatcca gacataaggc
    29521 ccggacggat ctttcttgtc tgctttcagc gcatcaacga tgccctggtt tgccggatcc
    29581 tggtcatagc gttttggcat agtgaccaac atgccttcgg cagcatcgcc agcaatattc
    29641 gacaatgatg cgttacccac accttccggc cccataaact gagttttcag gccaacggaa
    29701 cgggcctggc gcagcatctg ccccatttcc gggtagtaac cgccgtagta aacgaagtcg
    29761 atgttttctt ttttcaggcg ggcgatcagc gcggagaaat ctttctcccc ggcggtaata
    29821 ccgtcgaaga agacgacgtt ggcgttagcc gctttcagcc cgtcctgcac cgaacgcgcc
    29881 agcccttcgc catactgttg tttgtcgtga atgatggcga tgcgctgggg cttcaccgtc
    29941 tcaagaatgt attttgccgc cgtaggcccc tgggaagagt ccagcccggc agtacgcata
    30001 atgtgttgat aaccgcgttg ggtcagctcc gggttggtcg ctcccggcga gatcatcaga
    30061 ataccttcgt cttcgtagat atctgacgca ggttgggtag aagaagaaca cagatgacca
    30121 ataacgtatt taatgccgtc gttaacgatt ttgttggcga ccgcaacggc ttgtttcggg
    30181 tcgcaggcgt cgtcatattc cacgcccacc agcttatcgc ctttaattcc ccctttggca
    30241 ttaatgtctt taatcgcctg acgcgcgccg ttaaattcca tatcgcccca ctgggcaatc
    30301 gggccggaca tcgcgccgac aacggcaact ttaatatcgt cagccatagc ggtgtgtgaa
    30361 attgccagtg caatcatccc tgcgatgata gttttcgcat tccgtttcat agtcaaaaat
    30421 ccccattcgt gatgttgtgt tgctttgttt ttatgtgtta acaaatcaga ctgttctttt
    30481 tttatactgc actgtttttg cctgtctgat taagggttta gcgcagtatt ttgtgataat
    30541 agcgattaaa atccctattt ttcagtcgat taagaacaga taatattctg aatttattga
    30601 tagataaaca gaa
//
LOCUS       DBANUU010000051        30478 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000051 DBANUU010000000
VERSION     DBANUU010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.051"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(77..1327)
                     /gene="icd"
                     /locus_tag="ACIE68_002183"
     CDS             complement(77..1327)
                     /gene="icd"
                     /locus_tag="ACIE68_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFX5908494.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1499..2152
                     /gene="rluE"
                     /locus_tag="ACIE68_002184"
     CDS             1499..2152
                     /gene="rluE"
                     /locus_tag="ACIE68_002184"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFX5908495.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2162..2623
                     /gene="nudJ"
                     /locus_tag="ACIE68_002185"
     CDS             2162..2623
                     /gene="nudJ"
                     /locus_tag="ACIE68_002185"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFX5908496.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2677..3783
                     /gene="mnmA"
                     /locus_tag="ACIE68_002186"
                     /gene_synonym="trmU"
     CDS             2677..3783
                     /gene="mnmA"
                     /locus_tag="ACIE68_002186"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFX5908497.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3819..4460
                     /gene="hflD"
                     /locus_tag="ACIE68_002187"
     CDS             3819..4460
                     /gene="hflD"
                     /locus_tag="ACIE68_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFX5908498.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4464..5834
                     /gene="purB"
                     /locus_tag="ACIE68_002188"
     CDS             4464..5834
                     /gene="purB"
                     /locus_tag="ACIE68_002188"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFX5908499.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6003..6674
                     /gene="phoP"
                     /locus_tag="ACIE68_002189"
     CDS             6003..6674
                     /gene="phoP"
                     /locus_tag="ACIE68_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFX5908500.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHI
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6674..8134
                     /gene="phoQ"
                     /locus_tag="ACIE68_002190"
     CDS             6674..8134
                     /gene="phoQ"
                     /locus_tag="ACIE68_002190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFX5908501.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8210..9331
                     /gene="roxA"
                     /locus_tag="ACIE68_002191"
                     /gene_synonym="ycfD"
     CDS             8210..9331
                     /gene="roxA"
                     /locus_tag="ACIE68_002191"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFX5908502.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9380..10606)
                     /gene="pepT"
                     /locus_tag="ACIE68_002192"
     CDS             complement(9380..10606)
                     /gene="pepT"
                     /locus_tag="ACIE68_002192"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFX5908503.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVESYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            10856..11992
                     /gene="potA"
                     /locus_tag="ACIE68_002193"
     CDS             10856..11992
                     /gene="potA"
                     /locus_tag="ACIE68_002193"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFX5908504.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            11976..12833
                     /gene="potB"
                     /locus_tag="ACIE68_002194"
     CDS             11976..12833
                     /gene="potB"
                     /locus_tag="ACIE68_002194"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFX5908505.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12830..13624
                     /gene="potC"
                     /locus_tag="ACIE68_002195"
     CDS             12830..13624
                     /gene="potC"
                     /locus_tag="ACIE68_002195"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFX5908506.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13621..14667
                     /gene="potD"
                     /locus_tag="ACIE68_002196"
     CDS             13621..14667
                     /gene="potD"
                     /locus_tag="ACIE68_002196"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFX5908507.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            14725..15186
                     /locus_tag="ACIE68_002197"
     CDS             14725..15186
                     /locus_tag="ACIE68_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFX5908508.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            15183..15971
                     /locus_tag="ACIE68_002198"
     CDS             15183..15971
                     /locus_tag="ACIE68_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFX5908509.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(16110..16931)
                     /gene="cobB"
                     /locus_tag="ACIE68_002199"
     CDS             complement(16110..16931)
                     /gene="cobB"
                     /locus_tag="ACIE68_002199"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFX5908510.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(16947..17858)
                     /gene="nagK"
                     /locus_tag="ACIE68_002200"
     CDS             complement(16947..17858)
                     /gene="nagK"
                     /locus_tag="ACIE68_002200"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFX5908511.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(17887..19131)
                     /gene="lolE"
                     /locus_tag="ACIE68_002201"
     CDS             complement(17887..19131)
                     /gene="lolE"
                     /locus_tag="ACIE68_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFX5908512.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(19131..19832)
                     /gene="lolD"
                     /locus_tag="ACIE68_002202"
     CDS             complement(19131..19832)
                     /gene="lolD"
                     /locus_tag="ACIE68_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFX5908513.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(19825..21024)
                     /gene="lolC"
                     /locus_tag="ACIE68_002203"
     CDS             complement(19825..21024)
                     /gene="lolC"
                     /locus_tag="ACIE68_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFX5908514.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            21286..22359
                     /locus_tag="ACIE68_002204"
     CDS             21286..22359
                     /locus_tag="ACIE68_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFX5908515.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLLYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            22487..25933
                     /gene="mfd"
                     /locus_tag="ACIE68_002205"
     CDS             22487..25933
                     /gene="mfd"
                     /locus_tag="ACIE68_002205"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFX5908516.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARILLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            26077..27039
                     /gene="ldtC"
                     /locus_tag="ACIE68_002206"
     CDS             26077..27039
                     /gene="ldtC"
                     /locus_tag="ACIE68_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFX5908517.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(27122..27379)
                     /gene="bhsA"
                     /locus_tag="ACIE68_002207"
                     /gene_synonym="ycfR"
     CDS             complement(27122..27379)
                     /gene="bhsA"
                     /locus_tag="ACIE68_002207"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFX5908518.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            27620..28252
                     /gene="comR"
                     /locus_tag="ACIE68_002208"
     CDS             27620..28252
                     /gene="comR"
                     /locus_tag="ACIE68_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFX5908519.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(28314..28853)
                     /locus_tag="ACIE68_002209"
     CDS             complement(28314..28853)
                     /locus_tag="ACIE68_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFX5908520.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(29080..30384)
                     /gene="ndh"
                     /locus_tag="ACIE68_002210"
     CDS             complement(29080..30384)
                     /gene="ndh"
                     /locus_tag="ACIE68_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFX5908521.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT         7454 a         7918 c         7657 g         7449 t
ORIGIN      
        1 tgctattaat aatatccaca taaataaaac aacgggcgtg ttatacgccc gtttcaatat
       61 ttaacacatg gagagattac atattcttga tgatcgcgtc accaaactct gaacatttca
      121 gcaatttagc gccttccatc agacgttcga agtcataggt tacggtcttg gcattgattg
      181 cgccttccat acctttaaca atcaggtcag ccgcttcagt ccaacccata tggcgtaaca
      241 tcatctcagc agagagaata atagagcctg ggtttacttt gtcctgaccg gcatatttcg
      301 gcgcagtacc gtgggtggct tcaaacaggg cgcattcgtc accgatgttt gcacctgggg
      361 cgataccgat accgccaacc tgcgctgcca gggcgtcaga aatgtagtca ccgttcaggt
      421 tcatacaggc gataacatca tattcagccg gacgcagcag gatctgttgc aggaatgcat
      481 cagcaatcac gtctttaatg acgatctctt tgccggtgtt cgggttttta actttcagcc
      541 acgggccgcc gtcgatcagt tcaccgccaa actcttcacg cgccagctgg tagccccagt
      601 ctttaaacgc tccttcggtg aacttcatga tgttgccttt gtgcaccaga gtcacagagt
      661 cacgatcgtt agcaattgcg tattcgatcg ctgcacgaac cagacgtttg gtgccttctt
      721 ccgaacacgg cttaataccg ataccacaat gttccgggaa gcgaattttc ttcaccccca
      781 tctcttcacg caggaattta atcactttct cggcgtcggc agagtctgct ttccattcga
      841 tacccgcata aatgtcttcc gagttttcac ggaagataac catatcggtc agttcagggt
      901 gtttaaccgg gcttggagtg ccctgatagt aacgtaccgg acgcaggcag atgtagagat
      961 ccagttcctg gcgcagggca acgttcagag agcgaatacc gccaccaaca ggagtggtca
     1021 gcggaccttt aatggcaacg cgatattcac gaatcagatc aagggtttca gcaggcagcc
     1081 agacatcctg accataaacc tgtgtggatt tttcaccggt gtaaatttcc atccaggaga
     1141 ttttacgctc gcctttatag gctttctcga ctgcagcgtc gaccactttc agcatggctg
     1201 gggttacatc tacaccgatt ccatcacctt caatgtaagg gataatcgga ttttcaggaa
     1261 cgttgagttt gccgttttgc agggtgatct tcttgccttg tgccggaaca actactttac
     1321 tttccattca cctctccttc gagcgctact ggtttgctcg tctgccacca cgttgcatat
     1381 gcgtttgcgt cctgcgatac ggatgcttta gagcaatttt ttgttaatga tttgtaattg
     1441 gcttgtcaat actaccctat tgtttgccgt catgaaagat gcgcgttatt aggctataat
     1501 gcggcaattc ataatctctg aaaataccat gcaaaaaact tcttttagaa atcaccaggt
     1561 taagcgattc agctcgcaac gttctaccag gcgtaaacct gaaaaccagc ccacgcgtgt
     1621 gatcctgttc aataaaccct acgatgttct tccgcagttc accgatgaag ccggacgcaa
     1681 aacattaaaa gaattcatcc cggttcaggg cgtttatgca gcaggtcgcc ttgaccgcga
     1741 tagcgaaggg ttgctggtgc tgaccaataa cggcgcgttg caggcgcgtt taacccagcc
     1801 gggtaaacgc accggaaaaa tctattatgt gcaggtggaa ggtattccca cacaagacgc
     1861 acttgaagcc ttgcgcaatg gcgtaacctt aaatgatggc cctaccctgc ccgcaggcgc
     1921 ggagctggtt gacgaacccg cgtggttatg gccgcggaat ccaccaattc gtgaacgcaa
     1981 aagtattccc accagctggc tgaagatcac cttatatgaa ggacgtaatc gccaggtgcg
     2041 ccgcatgacc gcccatgttg gtttccccac gctgcgactg attcgctatg cgatgggaga
     2101 ttactctttg gataatcttg ccaatggcga atggcgagaa gtgacagatt aaggatgcaa
     2161 aatgtttaaa ccgcacgtta ccgttgcttg cgtggtgcac gcagaaggca aatttttagt
     2221 cgttgaagag acgattaatg gtaaagcgtt atggaaccaa cctgccgggc atctggaagc
     2281 cgatgaaacc ttagtggaag ccgccgcccg tgaactgtgg gaagaaaccg gcatcagcgc
     2341 gcagccgcaa cactttattc gtatgcatca gtggattgcg ccagataaaa cgccgttttt
     2401 gcgtttcctc tttgccattg agcttgagca aatatgcccg acccagcctc atgacagcga
     2461 tatcgactgc tgccgttggg tcagcgccga agaaatttta caggcgtcaa atcttcgttc
     2521 gccgctggtg gcggaaagta ttcgttgtta tcaaagcggg caacgttatc cgctggagat
     2581 gattggcgat tttaactggc cttttacaaa gggtgtcatc taaaaggctg cgtgatagaa
     2641 tacgccgcct tgaagttcaa tgtcgtgagt gatccaatgt ctgaaaccgc aaaaaaagta
     2701 atcgtcggca tgtccggcgg tgtcgattcc tccgtttctg cctggctgtt gcaacaacag
     2761 ggatatcagg tcgaaggcct gtttatgaag aactgggaag aagacgacgg tgaggaatat
     2821 tgcacagcgg cagcggatct ggctgatgcc caggctgtct gcgacaagct cggcattgaa
     2881 ctgcacaccg ttaactttgc tgccgagtac tgggacaacg tcttcgaact gttccttgcc
     2941 gaatataaag ccggtcgcac gccgaatccg gatattctgt gcaacaaaga gatcaaattt
     3001 aaagccttcc tcgaatttgc cgccgaagat ttaggtgccg attatatcgc taccggtcat
     3061 tacgtacgtc gggccgatgt cgatggcaag agccgcctgc tgcgtggtct ggacagcaat
     3121 aaagaccaga gctacttcct ttatacgctc agccatgagc agattgcgca aagcctgttc
     3181 ccggtcggcg aactggaaaa accgcaggtg cgtaagattg ctgaagatct tggtctggtc
     3241 accgcgaaga aaaaagactc taccggcatt tgcttcattg gcgaacgtaa attccgcgag
     3301 ttcctgggcc gttatctccc ggcgcaaccg ggcaaaatca ttaccgtcga tggcgatgaa
     3361 attggtgagc accaggggct gatgtatcac actctcggtc agcgtaaagg tctgggtatc
     3421 ggtggcacca aagaaggtac cgaagaaccg tggtatgtgg tggacaaaga cgtcgaaaac
     3481 aacattctgg ttgtcgctca gggccatgaa cacccgcggc tgatgtctgt cgggttgatt
     3541 gcccagcagt tgcactgggt cgatcgcgaa ccattcaccg gcactatgcg ttgcacggta
     3601 aaaacccgct atcgccagac cgatatccct tgcaccgtca aggcgctgga cgatgatcgc
     3661 attgaagtga ttttcgatga accggttgcc gccgtgacgc cgggccagtc tgccgtcttc
     3721 tataacggtg aagtgtgcct cggtggcggt attattgagc agcgtctgcc gctgccggtc
     3781 tgattattat ctttacttaa aacaaggaag cagtgaacgt ggcaaagaat tactatgaca
     3841 tcaccctcgc cctggccggt atttgtcagt cggcacgcct ggtgcaacaa ctcgctcacc
     3901 aggggcattg tgatgccgat gcgctacacg tctcactcaa cagtattatt gatatgaacc
     3961 ccagctcgac gctggcggtt tttggcggta gcgaagccaa cctgcgcgtc gggctggaaa
     4021 ccctgctcgg cgtgctcaat gccagcagtc gccagggctt aaacgccgaa ttaacccgct
     4081 acacactcag cttgatggtg cttgagcgca aactctcctc agcgaaaggc gcgctcgaca
     4141 ctctgggcaa ccggatcaac ggcctgcaac gccagctcga acacttcgat ttacagtccg
     4201 aaacgctgat gagcgcgatg gctgctatct atgttgatgt gattagcccg cttggcccgc
     4261 gcattcaggt caccggttcc cctgctgtac tgcaaagccc acaagtgcag gcgaaagttc
     4321 gcgcaaccct gctggcaggc attcgcgccg ccgtgctctg gcaccaggtc ggcggcggac
     4381 gtctgcaact gatgttttct cgtaatcgcc tgaccactca ggcaaaacaa attcttgctc
     4441 atttaacccc ggagttgtga tctatggaat tatcctcact gaccgccgtt tcccctgtcg
     4501 atggacgcta cggcgataaa gtcagcgcgc tgcgcgggat tttcagcgaa tatggtttgc
     4561 tgaaattccg tgtacaagtt gaagtacgtt ggctgcaaaa actggccgcg cacgcagcga
     4621 tcaaggaagt tcctgctttt gctgccgacg caatcggtta ccttgatgca atcgtcgcca
     4681 gtttcagcga agaagatgcg gcgcgcatca aaactatcga gcgtaccact aaccacgacg
     4741 ttaaagcggt tgagtatttc ctgaaagaaa aagtggcgga gatcccggaa ctgcacgcgg
     4801 tttctgaatt catccacttt gcctgtactt cggaagatat caataacctc tcccacgcat
     4861 taatgctgaa aaccgcgcgt gatgaagtga tcctgccata ctggcgtcaa ctgattgatg
     4921 gcattaaaga tctcgccgtt cagtatcgcg atatcccgct gctgtctcgt acccacggtc
     4981 agccagccac gccgtcaacc atcggtaaag agatggcaaa cgtcgcctac cgtatggagc
     5041 gccagtaccg ccagcttaac caggtggaga tcctcggcaa aatcaacggc gcggtcggta
     5101 actataacgc ccacatcgcc gcttacccgg aagttgactg gcatcagttc agcgaagagt
     5161 tcgtcacctc gctgggtatt caatggaacc cgtacactac ccagattgaa ccgcacgact
     5221 acattgccga actgtttgat tgcgttgcgc gcttcaacac cattctgatc gactttgacc
     5281 gtgacgtctg gggttatatc gcccttaacc acttcaaaca gaaaaccatt gctggtgaga
     5341 ttggttcttc caccatgccg cataaagtta acccgatcga cttcgaaaac tccgaaggga
     5401 atctgggcct ttccaacgcg gtattgcagc atctggcaag caaactgccg gtttcccgct
     5461 ggcagcgtga cctgaccgac tccaccgtgc tgcgtaacct cggcgtgggt atcggttatg
     5521 cgctgattgc gtatcaatcc accctgaaag gcgtgagcaa actggaagtg aaccgtgacc
     5581 atctgctgga tgaactggat cacaactggg aagtgctggc tgagccaatc cagacagtta
     5641 tgcgtcgcta tggcatcgaa aaaccgtacg agaagctgaa agagctgact cgcggtaagc
     5701 gcgttgacgc cgaaggcatg aagcagttta tcgacggtct ggcgttgcca gaagaagaga
     5761 aagcccgcct gaaagcgatg acgccggcta actatattgg tcgagctatc acgatggttg
     5821 atgagctgaa ataaacctcg tatcagtgcc ggatggcgat gctgtccggc ctgcttatta
     5881 agactatccg ctttttattt tttcacttta cctcccctcc ccgctggttt atttaatgtt
     5941 tacccccata accacataat cgcgttacac tattttaata attaagacag ggagaaataa
     6001 aaatgcgcgt actggttgtt gaagacaatg cgttgttacg tcaccacctt aaagttcaga
     6061 ttcaggatgc tggtcatcag gtcgatgacg cagaagatgc caaagaagcc gattattatc
     6121 tcaatgaaca tataccggat attgcgattg tcgatctcgg attgccagac gaggacggtc
     6181 tgtcactgat tcgccgctgg cgtagcaacg atgtttcact gccgattctg gtattaaccg
     6241 cccgtgaaag ctggcaggac aaagtcgaag tattaagtgc cggtgctgat gattatgtga
     6301 ctaaaccgtt tcatattgaa gaggtgatgg cgcgaatgca ggcattaatg cggcgtaata
     6361 gcggtctggc ttcacaggtc atttcgctcc ccccgtttca ggttgatctc tctcgccgtg
     6421 aattatctat taatgacgaa gtgatcaaac tgaccgcgtt cgaatacacc attatggaaa
     6481 cgttgatacg caataatggc aaagtggtga gcaaagattc gttaatgcta caactctatc
     6541 cggatgcgga gctgcgggaa agccatacca ttgatgtact gatgggacgt ctgcgcaaaa
     6601 aaattcaggc acaatatccg caagaagtga ttaccaccgt tcgcggccag ggctatctgt
     6661 tcgaattgcg ctgatgaaaa aattactgcg tctttttttc ccgctctcgc tgcgggtacg
     6721 ttttctgttg gcaacggcag cggtagtact ggtgctttcg cttgcctacg gaatggtcgc
     6781 gctgatcggt tatagcgtca gtttcgataa aactacgttt cggctgttac gtggcgaaag
     6841 caatctgttc tatacccttg cgaagtggga aaacaataag ttgcatgtcg agttaccaga
     6901 aaatatcgac aagcaaagcc ccaccatgac gctaatttat gatgagaacg ggcagctttt
     6961 atgggcgcaa cgtgacgtgc cctggctgat gaagatgatc cagcctgact ggctgaaatc
     7021 gaatggtttt catgaaattg aagcggatgt taacgatacc agcctcttgc tgagtggaga
     7081 tcattcgata cagcaacagt tgcaggaagt gcgggaagat gacgacgacg cggagatgac
     7141 ccactcggtg gcggtaaacg tctacccggc aacatcgcga atgccaaagt taaccattgt
     7201 ggtggtggat accattccgg tggagctaaa aagttcctat atggtctgga gctggtttat
     7261 ctatgtgctc tcagccaatc tgctgttagt gatcccgctg ctgtgggtcg ccgcctggtg
     7321 gagtttacgc cccatcgaag ccctggcaaa agaagtccgc gaactggaag aacataaccg
     7381 cgaattgctc aatccagcca caacgcgaga actgaccagt ctggtacgaa acctgaaccg
     7441 attgttaaaa agtgaacgcg aacgttacga caaataccgt acgacgctca ccgacctgac
     7501 ccatagtctg aaaacgccac tggcggtgct gcaaagtacg ctgcgttctc tgcgtagtga
     7561 aaagatgagc gtcagtgatg ctgaaccggt aatgctggag caaatcagcc gcatttcaca
     7621 gcaaattggc tactacctgc atcgtgccag tatgcgcggc gggacattgc ttagccgcga
     7681 gctgcatccg gtcgccccac tgctggacaa tctcacctcg gcgctgaaca aagtgtatca
     7741 acgcaaaggg gtcaatatct ctctcgatat ttcgccagag atcagctttg tcggtgagca
     7801 gaacgatttt gtcgaggtga tgggcaatgt gctggataat gcctgtaaat attgcctcga
     7861 gtttgtcgaa atttctgcaa ggcaaaccga cgagcatctc tatattgtgg tcgaggatga
     7921 tggacccggt attccattaa gcaagcgaga ggtcattttc gaccgtggtc aacgggttga
     7981 tactttacgc cctgggcaag gtgtagggct ggcggtagcc cgcgaaatca ccgagcaata
     8041 tgagggtaaa atcgtcgccg gagagagcat gctgggcggt gcgcggatgg aggtgatttt
     8101 tggtcgccag cattctgcgc cgaaagatga ataaatatgt ccatacttca cgcattacgt
     8161 taagcatccg ttataatcgg ttgcagatac cagcctgtgg atgcttaaca tggaatacca
     8221 actcactctt aactggcccg attttcttga acgtcactgg cagaaacgcc cggtggtgtt
     8281 aaaacgcggc tttaataatt ttattgaccc gatctctcca gacgagttgg cgggtctggc
     8341 gatggaaagc gaagtcgaca gtcgactggt cagtcaccag gatggcaaat ggcaggtcag
     8401 ccacggtccg ttcgaaagct acgatcatct cggtgaaact aactggtcat tgttagtgca
     8461 agcagtaaat cactggcatg agccgaccgc cgcgctgatg cgaccgttcc gtgaactacc
     8521 ggactggcgt attgatgatc tgatgatctc tttttctgta cctggcggcg gcgtcggccc
     8581 gcatctcgat cagtacgacg tgtttatcat tcagggtacc ggacgtcgtc gctggcgagt
     8641 gggcgaaaag ctgcaaatga aacagcactg cccacacccg gatctgttac aggtcgatcc
     8701 gttcgaagcc atcatcgatg aagagctgga gcctggcgat attctttata ttccgccagg
     8761 attcccgcat gaaggctacg cgctggaaaa tgcgatgaac tattccgtgg gttttcgcgc
     8821 gccaaatacg cgggaattaa ttagcggatt tgccgattat gtgctgcaac gtgaactggg
     8881 cggcaactac tacagcgatc cggatgttcc acctcgcgct catcctgcgg acgttctgcc
     8941 gcaagaaatg gataaactgc gtgagatgat gctcgaattg atcaaccagc cggaacactt
     9001 taagcaatgg tttggcgagt ttatatccca gtcacgtcat gaactggata tcgcgccgcc
     9061 ggaaccgcct tatcagccag atgaaatcta cgatgcgctg aaacaaggtg atgtgctggt
     9121 gcgcctgggt ggtctgcgcg tattgcgcat tggcgacgac gtgtatgcca atggtgagaa
     9181 gatcgattcc ccgcaccgtc cggcactgga tgcactcgcc agcaacattg cgctgaccgc
     9241 ggagaatttt ggcgatgcgc tggaagatcc gtcattcctc gcgatgctcg cggcgctggt
     9301 caatagcgga tactggttct tcgaagggta agtttggatt taagccggat gcggcatcac
     9361 atgccgcatc ccttttcgct tacttccgtt gcgccgttaa ctcggcaata cggacgatca
     9421 cctgcaccgc tttttccata ccttccagag tcacaaactc atgcttacca tgatagttgt
     9481 aaccgccagt gaacaggttc gggcacggta atcccataaa cgacaactgc gcgccgtcgg
     9541 taccgccgcg gatcggtttc agttccggtt caatatcgca gtcacgcatc gcctgctggg
     9601 cgatatcgag aatatgcgga tgctcaacca ctttctcgcg catattgtag taactgtctt
     9661 caatcaccag ttcaatgtag caatcaggat gtaacccttt gcccactttt ttggcgatct
     9721 ccatcatttt acgtttacgc gcttcaaact gtttacggtc gaaatcacgg atgatgtagt
     9781 gcatatcggc tcggtcaacg gtgcctttca tgctcgccag gtgatagaaa ccttcatagc
     9841 cttctgtcat ttccgggctt tcatccgccg gaacttccgc atgaatacgt gccgccagcg
     9901 acagcgcatt taccatcact cctttcgccg tgcccggatg aacattgtta ccgacaattt
     9961 tgatattgac cgatgcggcg ttgaagtttt caaactccag ttcgcctacg ccaccaccgt
    10021 caacagtgta agcccagcgg gcatcgaagg catcaacatc aaaatgtttc gcccctttgc
    10081 ccacttcttc atccggggta aaggcgacgc gaatatcacc atgcggaatg tttttctgtt
    10141 gcaataccgc cagcgcggtc atgatttctg caataccggc tttgtcatcg gcacctaaca
    10201 aggttttacc atcggtggta atcaacgtct gacccagtag ctgatgcagc accgggaaca
    10261 taaccggtga taaaacttca tcgccgatac ccagcgcaat atcgccaccg cgatagcttt
    10321 caacaatttg cggattaaca tttttgccgc tgcaatccgg tgaggtatcc acatgagaaa
    10381 taaagccaat cgccgggata tcgccaggga cgttagccgg taacgtcgcc atcaaagtgc
    10441 ccttctcact taaggtcaca ttgataagcc ccatttcttc gagctgctct ttcagcagat
    10501 gcaataactt ccattggcct tccgtgctgg gaacctgtct cacccctgct tttgattggg
    10561 tatccagaga cacgtagttc aaaaatcgct caagtagttt atccatgtag tcaccctcac
    10621 tttttgtgac aacattatta agaagcaaga aaagacaaat attgcgtcag gtcactttta
    10681 cccctgcaag cgggaatatt tatcagcatt accttaatga atataaagct aagccagtaa
    10741 ttcacaacaa agattggcga ttcaagcggt ttgccgtaga attaccgccc tcattaagag
    10801 tcaccaaggt ggttaaccac aaaccccgca tcggtaagcc atccgttgcg tttacatggg
    10861 acagagtaaa aaattgaata aacaaccgag ttcgctttca ccgctggtgc aattggcggg
    10921 aattcgcaaa tgctttgatg gtaaagaggt cattccccag ctggatctga ctatcaacaa
    10981 tggcgagttc ctcacgctgc ttggcccttc tggctgcggt aaaacaaccg ttcttcgcct
    11041 gattgcaggt ctggaaactg ttgattccgg acgcatcatg ctggataacg aggacatcac
    11101 ccacgttccg gcggaaaacc gctatgtgaa cactgttttc caaagctacg cacttttccc
    11161 ccacatgacc gtgttcgaaa atgtggcctt tgggttgcgc atgcaaaaaa cccccgctgc
    11221 tgaaattacg ccccgcgtga tggaagccct gcggatggtg caactggaaa ccttcgctca
    11281 acgtaaaccg catcagctct ctggtggtca acagcaacgc gtcgccattg ctcgcgcggt
    11341 ggttaacaag cctcgtctgt tgttgctgga tgagtcgctc tcagcgctgg attacaaact
    11401 gcgtaagcaa atgcagaacg agctgaaagc gttacagcgt aagcttggta ttaccttcgt
    11461 ctttgtcact cacgatcagg aagaagcgct caccatgtca gacaggattg tggtgatgcg
    11521 cgagggtcgt attgagcaag atggtacacc gcgcgaaatt tacgaagagc cgaaaaacct
    11581 gtttgttgcc ggcttcattg gcgaaatcaa tatgtttaac gccactgtca tcgaacgtct
    11641 agacgagcag cgcgtacgcg ccaacgttga aggccgcgaa tgtaatatct acgttaactt
    11701 cgccgttgaa ccggggcaaa aactgcatgt tctgctgcgc ccggaagact tacgtgttga
    11761 agagattaac gacgacaacc acgctgaagg gctgattggt tacgttcgtg agcgtaacta
    11821 caaaggcatg acgctggagt cggttgttga actggaaaat ggcaagatgg tgatggtcag
    11881 cgaattcttc aatgaagacg accctgactt tgaccactct ctcgaccaaa aaatggccat
    11941 taattgggta gaaagctggg aggtcgtact ggctgatgaa gaacacaagt aagttccaga
    12001 atgtagtgat tgtcactatt gtcggttggc ttgtgttgtt tgtctttctg cccaacctga
    12061 tgatcattgg caccagcttt ttgacccgcg acgacgccag tttcgtcaaa atggtcttta
    12121 cgctggataa ctacacgcgt ctgctcgatc cgctctattt tgaagtgcta ttgcactcgc
    12181 tgaatatggc gctgatcgcc accctcgcct gcctggtgct gggctacccg tttgcctggt
    12241 ttctggcgaa gctaccacac aaggtgcgtc cgctgctgct gtttctgctg attgttccct
    12301 tctggaccaa ctcactgatt cgcatctacg ggctgaaaat tttcctcagc accaaaggct
    12361 atctcaacga gtttttgctc tggctgggcg ttatcgacac accaattcgt atcatgttca
    12421 cgcccagtgc ggtgattatc ggtctggttt acattctgct accgtttatg gtgatgccgc
    12481 tgtactccag tatcgaaaaa ctggataagc cgttgcttga ggcggcgcgc gatctcggtg
    12541 ccagcaagtt acagactttt atccgtatca ttattccact gacgatgccg ggaattattg
    12601 ccggatgtct gctggtgatg ctgccagcga tgggcctgtt ctatgtatcc gacctgatgg
    12661 gcggtgcgaa aaacctgctg atcggtaacg tcatcaaggt ccagttcctc aatattcgtg
    12721 actggccgtt tggtgcagcc accagcatta cgctgactat cgtaatgggc ctgatgttgc
    12781 tggtttactg gcgcgcttct cgtctgctga ataaaaaggt ggaactcgaa tgatcggtcg
    12841 actgcttcgc ggcggtttta tgaccgctat ctacgcgtac ctgtatatcc caatcattat
    12901 tttgattgtg aactccttta acagttcgcg ctttggcatc aactggcagg gttttaccac
    12961 caaatggtat agcctgctga tgaacaacga cagcctgtta caggcagcgc agcattcact
    13021 aacaatggcg gtgttttcgg cgacatttgc tacgcttatc ggttcactga cggcagttgc
    13081 gctgtaccgt tatcgctttc gtggtaagcc gttcgttagc ggaatgctgt ttgtggtgat
    13141 gatgtcacca gatatcgtga tggcgatttc tctgctggtg ctgtttatgc tgctgggtat
    13201 tcagcttggc ttctggtcgc tgctgttctc gcatatcacc ttctgcctgc cgtttgtggt
    13261 ggtgacggtg tattcgcgcc tgaaaggttt tgacgtgcgg atgctggaag cggcgaaaga
    13321 tctcggtgcc agtgaattta ccattctgcg aaaaatcatt ctgccactgg caatgcctgc
    13381 ggtggcggcg ggctgggtgt taagctttac cctgtcgatg gacgatgtgg tagtttcttc
    13441 gttcgtcacc ggaccaagtt atgaaattct gccgttaaaa atttattcga tggtcaaagt
    13501 cggcgtatcg ccggaagtta acgcgctggc aaccatatta ctggtgctgt cgctggtgat
    13561 ggttattgcc agccagctta ttgctcgtga taaaacgaaa ggtaacacag gggacgttaa
    13621 atgaaaaaat ggtcacgcca cctgctcgcg gcgggtgctc tggcactggg catgagcgcc
    13681 gctcacgccg atgacaacaa cacgctgtat ttctacaact ggaccgagta cgtgccgcca
    13741 ggactgcttg aacagttcac caaagaaacc ggtattaagg ttatctattc gacttacgag
    13801 tcgaacgaaa ccatgtatgc caagctgaaa acgtacaaag acggtgccta tgacctggtg
    13861 gttccttcaa cctattacgt cgataaaatg cgtaaagaag ggatgatcca gaagatcgac
    13921 aagtcgaagt taacgaactt cagcaatctc gatccagaca tgctcaataa gccgtttgac
    13981 ccgaataacg attactccat tccgtatatc tggggtgcga cggcgattgg tgttaacggt
    14041 gatgcggtgg atccgaaatc tgtcaccagc tgggccgatc tgtggaagcc tgagtacaaa
    14101 ggcagcctgc tgttgaccga cgatgcccgt gaagtgttcc agatggcgct gcgtaagctg
    14161 ggctactccg gtaacaccac cgatccgaaa gagattgaag ctgcatataa cgagctgaaa
    14221 aaactgatgc caaacgtggc agcctttaac tccgataacc cggctaaccc gtacatggaa
    14281 ggcgaagtta acctcggcat gatctggaac ggttctgctt tcgttgcacg ccaggcgggt
    14341 acgccaattg acgtggtatg gccgaaagaa ggcggcattt tctggatgga cagcctggcg
    14401 atcccggcaa atgccaaaaa caaagaaggc gcgctgaaat tgatcaactt cctgctgcgc
    14461 ccggatgtgg caaaacaggt tgccgaaact atcggttatc caacgccaaa ccttgcggcg
    14521 cgtaagctgt taagcccgga agtggcgaac gataaaacgc tctacccgga tgctgaaacc
    14581 attaaaaatg gtgaatggca gaatgacgtt ggcgcagcca gcagcattta tgaagagtat
    14641 tatcagaagc tgaaagcagg acgttaattc agcaagcggg cggtggcatt accgcccgtt
    14701 ttttatttag caaggatgtg gctaatgagt caggacagta aagtgttatc ccgcgctttt
    14761 ctcggtatcg gtctgatatt gctcgttatt tctgcaatca tcttttatta ccattttact
    14821 tttaccaaaa gcgcggttca tacagaagga atcattgttg atgccgtttg gtacaacaat
    14881 cactcaaacg acgtcgatga taatggttcc tggtatccag tggttgcctt tcgccctacg
    14941 ccagattaca cgctaatttt caattcgaat atcggtagtg atttttatga agatagcgaa
    15001 ggtgacagag tcaatgtcta ttatccgcca gggcatccag aacaagccga aataaacaat
    15061 ccctgggtta atttttttaa atggggcttt gttggcatag caggcattat tttcggtagt
    15121 gtcggtctga taatcacgct cccctccacc aaaaaatctc gcaggaaacg gaagtcccgc
    15181 ccatgaaaaa attcattata ctgctcagtt tactgatact cctgcctctg gtagcaacca
    15241 gtaaaccatt gattccaata atgaagacac tttttacgga tgttaccggt actgtaccgg
    15301 atgctgaaga gatcgcacgt aaagccgagc tttttcgcca acaaacggga attgcaccgt
    15361 ttattgtgat tttacccgac attaataatg aagccagtct cagacaaaat ggtaaagcga
    15421 tgctggcgca tgcgtcatct tcattgagta atgtaaaagg aagtgttctg ttactattta
    15481 ctacccgcga accacggtta attatgatca ccaacggcca ggttgaaagt ggtctggacg
    15541 ataaacatct cggccttctg atagaaaatc acacgctggc ttatttaaat gcagatctct
    15601 ggtatcaggg aatcaataat gcattggctg ttctacaagc acagatatta aaacaaccga
    15661 cgccgccact gacgtattat ccgcatccag gacaacagca tgaaaatgct ccccccggca
    15721 gcaccaacac attaggtttt atcgcctggg ctgcgacgtt tatcttattt tcctggatct
    15781 tttgctacac gactcgtttt atttatgcgt taaaatttgc agtggcgatg actattgcca
    15841 atatgggtta tcaggcttta tgtttgtata tcgataatag ctttgcgatt accagaatat
    15901 cgccactctg ggcagggcta attggtgtct gtacgtttat tgccgcattg ttattgacga
    15961 gtaaacgata aaagaggccg gaaaagtgcg cgtgtattat gccggatggg gcgcgaacgc
    16021 cttatccggc ccacaaaacc cgcaaattca attaattgcg tccccttgta ggcctgataa
    16081 gcgtagcgca tcaggcaatg cttcctgctt tataatccct tcagcaactt ttcaacaaac
    16141 tcaggcacca cctggcttgc cgggccgtaa tatttctcgg caaattcatt accgacctga
    16201 cttggttcga gattcagttc cacggtgtgc gcgccatgca gtttcgcttc gtgaacaaac
    16261 ccagccgccg gataaacatg cccggaagta ccaatggcaa tgaaaatatc agccatcgac
    16321 aacgccatat aaatttcatc catgccgagt ggcatttcgc caaaccaaac gacgtgcggg
    16381 cgcaagggtg ccgggaactg gcaacaatgg catttatctt ctggcgtaac gtctccggtc
    16441 cagtcgagaa cctgaccact ttgcgaacaa cgaactttca gcaattcccc atgcatatga
    16501 atgacattgg tattacctgc gcgttcatgc aggttgtcta tattctgcgt taccagcaaa
    16561 aagcgatcgc cgagggcgtc ttgcagttta gccagcgcaa gatgcgcggc gttaggctga
    16621 atttctggct gctgcagctg tcgacgacgg gcgttataaa acgcttgcac cagttcagga
    16681 tcgcgatcga aaccttccgg cgttgccaca tcttcaaccc gatgttcttc ccacaggcca
    16741 tctgcggcgc gaaaggtacg aatacctgat tccgcagaaa tccctgcccc tgtcagtacg
    16801 agtactcttg gtttttccat tgcttccggc accactttat ctctgaaaaa aatacgctga
    16861 cgcaaacgct cgcgcaggcg gcgtttattt ttacgaaaac gacttaaccg atgaccccga
    16921 cgcgacagca tagcaacctc tgttgtttaa tcggttagat gtaggaaggc cgcaccacgc
    16981 attcctcccg catcaccgtg gcgcgcgcgt tcaatgcgcg gaacacgagc tacaggtaag
    17041 agatgacgag gcagcctgtc cgccagttgc gttgtgattg ccgggaaatt cgataagcca
    17101 ccaccaatga cgaccaggtc agggtcaaca atggtcagga tatttcccag acaaaccgct
    17161 aataaatcca gataacgctc aacgtgcgcc cttgcctgct catcgccttg atcataaagc
    17221 gcaataattt cgggagcctg caacggttga tgataatagt gttgatacag ccacgcaaaa
    17281 ccgcgaccag acagataatt ttcaatgcag ccatgctgac cacagccgca gcggcgtaac
    17341 gggaaatcca accccatcat ggttaacgca tcaaccggca gacgcatatg gccaaactcg
    17401 ccggtaatgt agcttttacc ggtaatcggt ttgccgttga aaatcagccc gccgccaacg
    17461 ccggtgccga gaatcaaccc catcaccagt ggatattgag taaattcatc atcccaggct
    17521 tctgaaaggg caaaacagtt ggcatcgtta tcaaggcgta catcgcgatc aagacgtgcg
    17581 ctcaggtcgg cacgcagcgg tttaccgctg gcagcgggga cattggcggc atacagcgta
    17641 ccatcttctg tttccggcat tcccggaata ccgatgccga cagaaccttt acagccaaaa
    17701 cgttggtcag cttcagctac cagctcacac actgcatcta aaaatgcgtc atagctgtca
    17761 cgcggtgtcg gcacccgctt ttcccactgc aactgccgac cgctatcaaa cacgccaagc
    17821 gcaatttttg ttccaccaat atcaaacccg taatacattg ccgctccttg ttttaatgta
    17881 ctgcctttac tggccgctaa ggactcgcgc agggtcaata ttactggcgc gccgcgccgg
    17941 ataccaactt gccaaaagac tcagcaacaa tgctgtgacc agtacgtaga agacgtccag
    18001 ccagtgcaat tccgatggca ggaaatcaat aaaatagata tcgctggaga ggaactgatg
    18061 accgatcaac ttttcaatcc actcaataat cggggtaagt tgcagtgaaa ccaccacacc
    18121 gataatcacc ccacacaggc tgccgaacag ccctgccagc aatccatacc agacaaagat
    18181 ggcgcgaatt aaaccatctt tcgcccccag cgttcttaat actgcgatat cgccactctt
    18241 gtctttcacc gccatcacta aggtggagac gatgttgaaa caggccacgc caatcaccag
    18301 taccatcgcc agatacataa tggcgcggat catctggata tcgcgataca tatagccgta
    18361 agtaccaatc caacttttaa tataaacata gctgttggtc acttcacccg catcgcgtac
    18421 cagcttattg gcgttgaaaa catccgtcat tttaagggca atacctgaca cgctggaacc
    18481 catatcaaga tattgttggg catccgccag cgggatcatg gcaaaactgt gatcgagttg
    18541 accactcaac tgcaaaatac cggcaatgtg caaacgcaca cgttttggct gcatcagttt
    18601 atgctcggga ttcgagttgg ggatcatgat cgacacccaa tcgccctgct tcactttcag
    18661 cgcatccgcc acgcctttgc cgatgataat ttgctgttcg cccgctttaa aattgcgcca
    18721 ggcatccccc tgaacaaacg agggtaatgc gctcagacgc tgttcctgtt gcgggttaac
    18781 gcccttcacc tgaattgcgc gcagattggc accactttct accagtccgg tgaaattgat
    18841 atacggcgca gcagcggcaa tacccggcac tttttgcacg ttatccagtg cttcctgcca
    18901 gttagtccat ggctgattta ccgcctcaat ttcaccatgc ggcaccaccg ccagaatacg
    18961 gttattcagt tcgcgttcaa agccgttcat cgcgcttaag ccgacgatca ataccgccac
    19021 gccgagggca atgccaatgg tagaaataac ggagatcagc gacaccatgc cgccgcgccg
    19081 ccgtccgcga ctaaaacgca ggccaatcaa taacgataaa ggcatcgcca ttactccgcc
    19141 cccatcaggc tcagttccgc cgtcagacgt ccatcacgca tttccaattg gcggctcata
    19201 cgtttcgcca gttgcaggtc gtgagtaacc accaggaagg cggtgccctg caagcgattc
    19261 aattccccaa gcaactggaa gatgctgtcg gcgttacgcg catcgaggtt accggtaggc
    19321 tcatccgcaa gtaccagacg cggattattg accagcgcac gggcaatcgc cacacgctgg
    19381 cgttcgccgc cagaaagttc agatgggcgg tgattcgcac gatgctccag ccccaccgct
    19441 tttaacatct ccagcgcacg gctgttgatt tcagcgggct ttttcttgcc aatcagcagt
    19501 ggcatagcca cgttttccag ggcagtaaaa tccggcagca ggtggtgaaa ctgataaata
    19561 aagcccagct tctggttgcg cagttctgct ttcgccgccg aagacagttt gctcatcggt
    19621 tgaccgttaa agatcacatc gccggaggtt ggtgtatcca gcccgcccag caggtgcagc
    19681 aaggtacttt taccggaacc agagctaccg acgatcgcca tcatttcacc ttcgcccacg
    19741 ctgaaactga cattgtgcag tacatcggtt tgcacactgc cttcctgata gcgtttgcac
    19801 aggttgtcgc attgcaacag gatcttattc ataacgtaaa gcctcagcgg gttgtgtggc
    19861 ggcagcgcgc catgaagggt aaagcgtaga cagcagcgcg atagccatcg ccaccagcgc
    19921 aataacaatg acctgtaaag gttcgatagc caccggcagc gccgcgccat caagcaggac
    19981 gccgattatc ggcatcagat tatttaactg gctggcaagc agtgcgccaa gcgccgcacc
    20041 gaggatcgca ccgataatcc cggcgctggc cccttgcacc ataaagacca tcatgatttg
    20101 tcgcggagtt aagccttgcg tttgcaggat cgcgacttcg ccctgcttct ccattaccat
    20161 cagccctagt gaggtaataa tgttaaacgc cgcaacggcg acaatcaggc tcagcagcaa
    20221 gcccatcata tttttttcca tgcgtacggc ctggaacagc tcgcctttac ggtcacgcca
    20281 gtcctgccat ttgctgcctt caggcagttt ttgctgactt aaagagtcaa ctttcagcgg
    20341 ctcatccagc cacaaacgcc agccggtaat attgcctgcc ggataacgca tcagacgcga
    20401 ggcatcctca atattcacca gcatttcata gccatcgact tcactgttgg cggcgaaagt
    20461 gccaatcaca ttgaacaggc gctggcttgg aatacgcccc atcggcgtga actggctggc
    20521 agatggtacc atcacgcgga tttgatcacc gcgattaacg cctagctgtg aggcaagttg
    20581 ttcgccgagg atgacattat atttccccgg ctcgaggtca gtttgtttca cattgaccag
    20641 atacggtgta agtggatctt tttgcgccgg gtcgataccg agcatcaccc cgaccgccac
    20701 gctgcgcgcg ctttgcagta ccacatcacc ggtagtaata ggtgcgacgc gattaacgcc
    20761 gtccagtttg actgccgttt ccgggagttg ctgcgggtta agagagccat gctcagaaga
    20821 gagaattgcc tgtggcatca ggccaaggat gttgttttgc agctcgcgct caaagccgtt
    20881 catcactgac aatactgtga ccagcgccat caccccgagg gtaatgccga tggtagaaag
    20941 ccaggagacg aaacgaccga agcgatccgc tgcacgccca cgcatgtaac gcaggccaat
    21001 aaatagagcg acaggttggt acatgaaatc cgtctggttg ctgtagcaaa gacacgagta
    21061 tataagcgaa agcaagatgg gtaaacgtct gaactgtaag tgatcgtcag ggctaatctg
    21121 aaaagccccc gtaaatcaat cggctaaatt gccatgtttt atcatcaatc ttgccgcctg
    21181 atcttaatct gaaaaatcat ccaatacaga cagataccca ttacacctcg ggtcataacc
    21241 cgcaggatca accgcatgta aattaaaaaa gagcgtgttg tcctgatgaa acaaaaagag
    21301 ctatggatta accagatcaa agggttatgt atttgtctgg tggtgattta tcactcggtc
    21361 attacctttt atccgcatct gaccactttt cagcatccgt tatcggaagt cctgagcaaa
    21421 tgctggatct atttcaatct ttaccttgcc ccctttcgta tgccggtttt tttctttata
    21481 tccggctatt tgattcgccg ctatatcgac agcgtaccgt ggggaaattg tctcgataaa
    21541 cggatctgga acatcttctg ggtgctggcg ctttggggcg tggtgcagtg gctggcgtta
    21601 agtgcgctga accagtggct ggcacctgag cgcgatttaa gcaatgcctc caatgccgct
    21661 tatgccgatt ccaccggtga gttcctgcac gggatgatca ccgccagcac cagcttgtgg
    21721 tatctgtatg cgttaattgt ctatttcgtg gtatgtaaaa tttttagccg tctggcgctg
    21781 ccactattcg ccttgtttgt actgctgagt gtggcggtta atttcgttcc cacgccgtgg
    21841 tggggaatga acagtgtgat ccgcaatttg ctttattaca gccttggcgc atggtttggc
    21901 gcaacaataa tgacctgtgt taaagaggtg ccgttgcgcc gccatctgct gatggcttct
    21961 ttgcttaccg ttctggcggt cggtgcctgg ttgtttacta tctcgctgct gttgtcgctg
    22021 gtatcgattg tggtgatcat gaagctgttt tatcaatacg agcaacgatt cggtatgcgc
    22081 tccaccagcc tgctgaatgt gattggctcc aacaccattg ctatctacac cacccatcgc
    22141 attctggttg aaatattcag cttaactctg cttgcgcaaa tgaacgcagc acgctggtcg
    22201 ccgcaagtcg aactgacact cctgctggtt tatccctttg tcagtttgtt catctgtact
    22261 gttgcgggct tgctggtaag aaaactttca cagcgtgcat tcagcgatct gttgttctcc
    22321 ccgccttcac tgcccgctgc cgtcagttac tctcgttaaa ccatcgcccc agccggggcg
    22381 attcaatttg ctaaaccatg tcgattacgg ataatgaaca gcattgctta tcagagatat
    22441 gcccccatat gttgaggcat atcctaacga gaatctgaca accgttatgc ctgaacaata
    22501 tcgttatacg ctgcccgtca aagcgggtga gcagcgtctg ctgggcgagt taaccggcgc
    22561 agcctgtgca acgctggtag cggaaattgc cgaacgtcac gccggtccgg tggtactcat
    22621 tgcaccagat atgcaaaatg ctctgcgttt gcatgatgaa atcagccagt tcaccgatca
    22681 gatggtgatg aatctggcgg actgggaaac tcttccctac gacagttttt cgcctcatca
    22741 ggacattatc tcctcgcgcc tttccaccct ttaccagcta ccgacgatgc agcgtggcgt
    22801 actgattgtt ccggtgaata cgcttatgca gcgcgtttgc ccacacagtt ttctccacgg
    22861 tcatgcgctg gtgatgaaaa aaggtcagcg cctgtcacga gatgcattac gaacccaact
    22921 ggacagcgcc ggttatcgcc atgttgacca ggtgatggag cacggcgaat acgccacgcg
    22981 cggcgcgttg ctggatctct tcccgatggg gagtgagctg ccttatcgtc ttgatttctt
    23041 tgatgatgaa atcgacagcc tgcgggtgtt tgacgtcgac agccagcgca cgctggagga
    23101 agtagaagcg atcaatctgc tgcccgcgca cgaatttccg accgataaag cggcaattga
    23161 actgttccgc agccagtggc gcgatacctt cgaagtgaag cgcgatccgg aacatattta
    23221 ccagcaagtg agtaaaggca cattacctgc cgggatcgag tactggcagc cattgttctt
    23281 cagcgaacca ctgccgccgc tgttcagtta tttccctgcc aataccttgc tggtgaatac
    23341 tggcgatctg gaaaccagtg ccgaacgttt ccaggctgac acgctggcgc gttttgagaa
    23401 tcgcggcgtc gatccgatgc gcccgctgtt gccaccacaa tcgctctggc tgcgggtgga
    23461 cgagctcttc tcagagctga aaaactggcc ccgggtgcag ctaaaaactg aacatttacc
    23521 gacaaaagcc gcgaatgcca atttaggttt ccagaaactg ccagacctgg ccgttcaggc
    23581 acaacaaaaa gcgccgctgg atgcgctgcg taagttcctc gagactttcg acggtccggt
    23641 ggtgttctcg gtagaaagtg aaggtcgccg tgaagcgctg ggtgaactgc tcgcacgaat
    23701 taaaattgct ccgcaacgca ttatgcgtct tgatgaagcc agcgaccgtg ggcgttatct
    23761 gatgattggc gctgccgaac atggttttgt cgataaggtg cgtaatctgg cgctgatctg
    23821 cgaaagcgat ctgctcggtg aacgtgttgc ccgccgtcgt caggattctc gccgcaccat
    23881 caaccccgat acactgatcc gtaaccttgc ggagctgcat attggtcagc cggtggtcca
    23941 tctggagcac ggtgtcgggc gctatgccgg aatgaccacg ctcgaagcgg gcggcattac
    24001 tggcgagtat ttgatgctca cctatgccaa cgacgccaaa ctgtatgttc cggtgtcgtc
    24061 actgcatctg attagccgtt acgcaggtgg cgcggaagaa aacgccccgc tgcataaact
    24121 tggcggcgat gcgtggtcac gcgcgcggca gaaagcggcg gaaaaagtgc gtgatgtggc
    24181 ggcggaattg ctggatatct acgcgcaacg cgccgccaaa gagggcttcg cgtttaaaca
    24241 cgatcgtgag cagtatcagt tgttctgcga cagcttcccg tttgaaacca cgccggatca
    24301 ggcgcaggcc attaatgcgg tacttagcga catgtgtcag ccgctggcaa tggatcgtct
    24361 ggtgtgcggc gatgttggct ttggtaaaac agaagtggcg atgcgcgccg ctttcctggc
    24421 agtagataac cacaagcagg tggcggtgtt ggtgcctacc acccttctcg cgcagcagca
    24481 ttacgacaac ttccgcgacc gtttcgccaa ctggccggta cgtatcgaaa tgatctcccg
    24541 tttccgcagc gccaaagagc agacgcaaat ccttgcggaa gtggcggaag ggaaaatcga
    24601 tattctgatc ggtacgcaca aactgctgca aagtgacgtc aagtttaaag atttaggcct
    24661 gctgattgtc gatgaagaac accgcttcgg ggtgcgtcat aaagagcgta ttaaagcgat
    24721 gcgcgcgaac gtggatattc tgacgcttac tgcaacgccg atcccacgta cgctgaatat
    24781 ggcaatgagc ggaatgcgtg acctgtcgat tatcgccacg ccgcccgccc gtcgtctggc
    24841 agttaaaacc tttgtccgtg agtatgacag cctggtggtc cgggaggcga tcctgcgtga
    24901 aattttgcgc ggaggacagg tttattatct ctacaatgat gtggaaaaca ttcagaaagc
    24961 cgccgaacgg ctggcagaac tggtgccaga agcgcggatc gccatcggtc acgggcagat
    25021 gcgcgagcgc gaactggaac gggtgatgaa tgatttccat catcaacgtt tcaacgtgct
    25081 ggtttgtacc accattatcg aaaccgggat cgacatcccg acagccaaca ccattatcat
    25141 tgaacgtgcg gatcacttcg gtctggcgca gctgcaccag ttacgcggtc gcgtcggacg
    25201 ttcgcatcat caggcatatg catggttgct gacgccgcat ccaaaagcga tgactaccga
    25261 tgcacaaaaa cgtcttgaag cgattgcctc gctggaagat ctcggtgcag gctttgcgct
    25321 ggcaacgcac gatctggaga ttcgcggcgc gggtgaactg cttggcgaag aacaaagcgg
    25381 ctcaatggaa accatcggtt tctcgctgta tatggagttg ctggaaaacg ccgtcgatgc
    25441 actgaaagcc ggacgcgagc cgtcgctgga agatctcacc agccagcaaa cagaagtcga
    25501 gctgcggatg ccgtcgctat tgccagatga tttcattcct gacgtgaata cgcgtttgtc
    25561 gttctataaa cgtattgcca gcgccaaaac ggaaaacgaa ctggaagaga tcaaagtcga
    25621 gcttatcgat cgcttcggcc tgctgccgga tccggcgcgt atcctgctgg atgttgcccg
    25681 tctgcgccag caagcgcaga aactggggat caggaagctg gaaggtaatg agaaaggcgg
    25741 ggtgatcgaa tttgccgaga agaatcacgt taatccggcc tggttgattg gtttgctgca
    25801 aaaacagccg cagcattacc gccttgatgg tccgacgcgc ctgaaattta ttcaggattt
    25861 gagtgagcgg aaaacgcgta tcgaatgggt acgccagttt atgcgtgaac tggaagagaa
    25921 cgcgatcgct taatggtaaa tcgggggcgt ttctgcgccc ccatctgcaa catttacaaa
    25981 ttctttgcac ttccctgcac tatccgacac tgtcaccatc cataattcag gcttatctgt
    26041 ttattacaat aaccttatat ttattatgga tttttggtga tgatcaaaac gcgtttttct
    26101 cgctggctaa cgttttttac gttcgccgct gccgtggcgc tggcgctacc ggcaaaagcc
    26161 aacacctggc cgctgccgcc agcgggcagt cgtctggttg gcgaaaacaa atttcatgtg
    26221 gtggaaaatg acggtggttc tctggaagcc atcgccaaaa aatataacgt cggctttctc
    26281 gctctgttac aggctaaccc cggcgttgat ccttacgtac cgcgcgcggg cagcgtgtta
    26341 acgatcccgt tgcaaaccct acttccagac gcgccgcgcg aaggcattgt gatcaacatt
    26401 gcggagctgc gtctctatta ctacccgccg ggtaaaaatt cggtaaccgt gtatccaata
    26461 ggtattggtc agttaggtgg tgacacgctg acaccgacaa tggtgaccac cgtttcagac
    26521 aaacgtgcaa acccaacctg gacgccaacg gcaaacatcc gcgcccgtta taaagcacag
    26581 ggaattgagt tgcctgcggt agtgccggct ggaccggata acccaatggg ccatcatgcg
    26641 attcgtctgg cggcctatgg cggcgtttat ttgcttcacg gtacgaacgc cgatttcggc
    26701 attggcatgc gggtaagttc tggctgtatt cgtctgcggg atgacgatat caaaacactc
    26761 tttagccagg tcaccccagg caccaaagtg aatatcatca acactccgat aaaagtctct
    26821 gccgaaccaa acggtgcgcg tctggttgaa gtacatcagc cgctgtcaga gaagattgat
    26881 gacgatccgc agctgctgcc aattacgctg aatagcgcaa tgcaatcatt taaagatgca
    26941 gcacaaactg acgcagaagt gatgcaacat gtgatggatg tccgttccgg gatgccggtg
    27001 gatgtccgcc gtcatcaagt gagcccacaa actctgtaaa tcctggataa aaaaaatccc
    27061 cgcgacacgg catgtcgcgg ggaaaaaatc agcagtagca ggcattaatg agggttaatg
    27121 cttatttata aattactgct gttccatgga gggtattcgg accggttaca gaagtaatac
    27181 ggaaagattt tgcgcccatc tcatccgctt tttgcgccag ctgctcttcc agcgatccca
    27241 gatttgtccc cgcgttagca ctgattgtac cgactttttg ttggccttct ggcgttgact
    27301 gaacttcgac agccgcaaag ctggcaaatg acatggagct taaaatcgcc gcagcgatga
    27361 gggtttttac gtttttcata atagtggcct tatgcagatg aatgacgtcg caagcaatta
    27421 cttaacgatc gataactaaa tgatagatgt gatctggatc acatacaaga tattttttat
    27481 aacaatcatt aattaattta aaaatcatta aatttcagtt aaatagaaag caattatttt
    27541 tatgggatct ggactggtga acggaggtga ttatttttat aataaccttt cattcacagt
    27601 caccaggtac aacggcatca tggcaactga ctcaacacaa tgtgtaaaaa aaagccgtgg
    27661 ccgcccaaaa gtgttcgaca gggatgccgc gcttgataag gccatgaaat tgttctggca
    27721 acacggttat gaagcgactt ctcttgcgga cctcgtcgaa gcgaccggag ccaaagcgcc
    27781 cacgctatac gcggaattta ccaacaaaga ggggttattt cgtgccgttc tcgaccgcta
    27841 tatcgatcgt tttgccgcta agcatgaagc acagctgttt tgtgaagaga aaagcgtgga
    27901 gtctgcgctg gctgactatt ttgctgccat cgccaactgc tttaccagca aagacacccc
    27961 ggcaggctgc ttcatgatca acaactgcac caccctctcc ccagattcag gagatatcgc
    28021 caatacgttg aaatcacgcc atgcgatgca agagcgcact ttgcagcagt ttttatgtca
    28081 acgacaagcg cgcggggaaa tcccgaccca ctgtgacgtg acacatctgg cagaattcct
    28141 taattgtatt attcagggga tgtcgatcag cgcacgcgaa ggtgcatcgc tggaaaaact
    28201 aatgcagatt gccggaacga ctttgcgttt atggcccgaa ctggtgaaat aagtcgcaaa
    28261 aaaagcccct cagcctgagc gaatgagggg ccaaattgca gagtacagcc ttgttatact
    28321 ttgttattca aaatcagttg cccattacta tctagcggga tctgcgtacc cggatcgcgg
    28381 tccatgcgga ttttgccctg ctgatcgcca atcttatagg tcacatcata accgagcatt
    28441 ttttctgact tgtcatacac cgttttacaa cgctgttgcg tagtcgtgta agtatcgctt
    28501 tcctggagag agccctggat ctggttaccg gcatatccac cacccagcgc ccccacaaca
    28561 gtggcgacat ctttaccacg accaccacca aactgatgcc ctatcacgcc gccagcaaca
    28621 gcgccgagca ccgacccggt aatgcgattt tcatcctgca ccggtcgacg atgggtcact
    28681 gtgacgttgc gacactcctg acgcggtgtt ttaaccgttt ccttgattgg ggttgcagaa
    28741 acaacctgag cgtattgcgg gccccgttca aacacgttca gactggccac tgccgctacg
    28801 cccagcgcag ctgcgacacc aatcccgata cccgccaaca ttgatttatt cacgggaaca
    28861 cctccttctt tgcagttatg ccaatcatgc aagcgaagga gaaaagcagc tatcagacag
    28921 aagatgaaaa gcgcgggaat ggctggaaat acttaaggat tcggaggagt ctgaaggaat
    28981 gcctgatgcg acgcttaacg cgtcttatca ggcctaccgc tcgtaccatt tgtccagggc
    29041 ggatgaggcg tttatgccac atccgccagt gtacgtcgat taatgcaact tcaaacgcgg
    29101 acggataacg cggttaatac tccccaccag catcattaat ccggttttaa agtaaccatg
    29161 cagcgcaatc tgatgcattc ggtatagcga gatatataca aagcgcgcaa ttcgtccttc
    29221 aatcatcatt gagccgcgcg tcaggttacc catcaggcta ccgacggtgg agaagttcga
    29281 cagcgatacc agcgaaccat gatctttata ctgataattt ttcagcggct taccgttcat
    29341 ctgcgccagt atgttgttca ttgcgcaagt cgccatctgg tgtgcagcct gagcacgcgg
    29401 cggaacaaag cccccttccg gacgcgggca tgacgcgcag tcgccaatag cgtaaatgtc
    29461 tggatcgcgg gtggtttgca gcgtcggttc caccaccagc tggttgatac ggttagtttc
    29521 aagaccaccg atatctttca ggaagtctgg cgctttgatc ccggctgccc acaccatcag
    29581 atcagcctca atatattcgc catctttagt gtgcaggccg ccttcatcag cactggtgac
    29641 catggtttgc gtcagcacgc gaacgccaag tttcgttagc tcgttgtggg ccgcagcaga
    29701 gatacgtggc ggtaatgcag gcaaaatacg ttctcccgct tctaccagcg ttacgttcag
    29761 ggcttcgttg gtcaggcctt tgtaaccgta gctgtgcagt tgcttgaccg cgttgtgcaa
    29821 ttcagcggag agttctacac ccgtcgcgcc gccgccgaca atcgcaatgt tcactttgcc
    29881 atttgcgccc aggttggcgg agtatttcag gaagagattc agcatctcct ggtgaaagcg
    29941 acgcgcctgg tgcgggttat cgaggaaaat gcagttctct ttgacacctg gcgtattgaa
    30001 atcgttagag gtgctaccca gcgccattac cagggtgtca taggcgattt tacgttccgg
    30061 aaccagcagt tcacctttct catcgcgcag ttctgcaata gtgattgttt tcgcttcacg
    30121 atcaatatca atgacggaac ccagctggaa ctggaaacca tgattgcgcg catgggccag
    30181 atagctcaac gcatcgacgc cttcatcaag cgagccagtc gccacttcgt gcagcagcgg
    30241 tttccacagg tggctgtggt tacgatcgac cagcgtaatt ttggcttttt tcttacgtcc
    30301 cagcttatgc cccagctgtg ttgccatttc cagcccacca gcaccgccgc cgacaatcac
    30361 aatctttttc aatggcgtag tcaacgtgac ccccttaaat ttattaacca attgttaatt
    30421 aatggttatt aacatagctc ttaattaaca acaggttaca gcaatgaaaa tgttcttc
//
LOCUS       DBANUU010000052        30291 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000052 DBANUU010000000
VERSION     DBANUU010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.052"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..292)
                     /locus_tag="ACIE68_002211"
     CDS             complement(<1..292)
                     /locus_tag="ACIE68_002211"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFX5908522.1"
                     /translation="MLEKSLATLFALLILATLINHFLLWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLF"
     gene            complement(292..897)
                     /locus_tag="ACIE68_002212"
     CDS             complement(292..897)
                     /locus_tag="ACIE68_002212"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFX5908523.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(1196..2056)
                     /gene="pdxI"
                     /locus_tag="ACIE68_002213"
     CDS             complement(1196..2056)
                     /gene="pdxI"
                     /locus_tag="ACIE68_002213"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFX5908524.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRIMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(2287..2877)
                     /gene="paaY"
                     /locus_tag="ACIE68_002214"
     CDS             complement(2287..2877)
                     /gene="paaY"
                     /locus_tag="ACIE68_002214"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFX5908525.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            complement(2859..3809)
                     /gene="paaX"
                     /locus_tag="ACIE68_002215"
     CDS             complement(2859..3809)
                     /gene="paaX"
                     /locus_tag="ACIE68_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFX5908526.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVVDNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(3910..5223)
                     /gene="paaK"
                     /locus_tag="ACIE68_002216"
     CDS             complement(3910..5223)
                     /gene="paaK"
                     /locus_tag="ACIE68_002216"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HFX5908527.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(5250..6455)
                     /gene="paaJ"
                     /locus_tag="ACIE68_002217"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(5250..6455)
                     /gene="paaJ"
                     /locus_tag="ACIE68_002217"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFX5908528.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(6455..6877)
                     /gene="paaI"
                     /locus_tag="ACIE68_002218"
     CDS             complement(6455..6877)
                     /gene="paaI"
                     /locus_tag="ACIE68_002218"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFX5908529.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(6867..8294)
                     /gene="paaH"
                     /locus_tag="ACIE68_002219"
     CDS             complement(6867..8294)
                     /gene="paaH"
                     /locus_tag="ACIE68_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFX5908530.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAANLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(8296..9084)
                     /gene="paaG"
                     /locus_tag="ACIE68_002220"
     CDS             complement(8296..9084)
                     /gene="paaG"
                     /locus_tag="ACIE68_002220"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFX5908531.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(9084..9851)
                     /gene="paaF"
                     /locus_tag="ACIE68_002221"
     CDS             complement(9084..9851)
                     /gene="paaF"
                     /locus_tag="ACIE68_002221"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFX5908532.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATD
                     TSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(9848..10918)
                     /gene="paaE"
                     /locus_tag="ACIE68_002222"
     CDS             complement(9848..10918)
                     /gene="paaE"
                     /locus_tag="ACIE68_002222"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFX5908533.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYARDHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIAATLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(10926..11423)
                     /gene="paaD"
                     /locus_tag="ACIE68_002223"
     CDS             complement(10926..11423)
                     /gene="paaD"
                     /locus_tag="ACIE68_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFX5908534.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMSTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVCCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(11438..12184)
                     /gene="paaC"
                     /locus_tag="ACIE68_002224"
     CDS             complement(11438..12184)
                     /gene="paaC"
                     /locus_tag="ACIE68_002224"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFX5908535.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(12193..12480)
                     /gene="paaB"
                     /locus_tag="ACIE68_002225"
     CDS             complement(12193..12480)
                     /gene="paaB"
                     /locus_tag="ACIE68_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFX5908536.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(12492..13421)
                     /gene="paaA"
                     /locus_tag="ACIE68_002226"
     CDS             complement(12492..13421)
                     /gene="paaA"
                     /locus_tag="ACIE68_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFX5908537.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            13706..15751
                     /gene="paaZ"
                     /locus_tag="ACIE68_002227"
     CDS             13706..15751
                     /gene="paaZ"
                     /locus_tag="ACIE68_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFX5908538.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RLFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDEAPAVH
                     ATEAFGPVATLMPAQNQRHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            15999..18272
                     /gene="tynA"
                     /locus_tag="ACIE68_002228"
     CDS             15999..18272
                     /gene="tynA"
                     /locus_tag="ACIE68_002228"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFX5908539.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQRDDYAQLFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDSRDRVAPAVK
                     PMQIIEAEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPSERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(18330..19829)
                     /gene="feaB"
                     /locus_tag="ACIE68_002229"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(18330..19829)
                     /gene="feaB"
                     /locus_tag="ACIE68_002229"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HFX5908540.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            20065..20970
                     /gene="feaR"
                     /locus_tag="ACIE68_002230"
     CDS             20065..20970
                     /gene="feaR"
                     /locus_tag="ACIE68_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFX5908541.1"
                     /translation="MNPAMDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(21142..21471)
                     /locus_tag="ACIE68_002231"
     CDS             complement(21142..21471)
                     /locus_tag="ACIE68_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFX5908542.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(21476..21661)
                     /locus_tag="ACIE68_002232"
     CDS             complement(21476..21661)
                     /locus_tag="ACIE68_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFX5908543.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(21658..24297)
                     /locus_tag="ACIE68_002233"
     CDS             complement(21658..24297)
                     /locus_tag="ACIE68_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFX5908544.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQSPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            24505..25494
                     /gene="ldhA"
                     /locus_tag="ACIE68_002234"
     CDS             24505..25494
                     /gene="ldhA"
                     /locus_tag="ACIE68_002234"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFX5908545.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            25605..26027
                     /gene="hslJ"
                     /locus_tag="ACIE68_002235"
     CDS             25605..26027
                     /gene="hslJ"
                     /locus_tag="ACIE68_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFX5908546.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(26024..26290)
                     /locus_tag="ACIE68_002236"
     CDS             complement(26024..26290)
                     /locus_tag="ACIE68_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFX5908547.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            26564..30088
                     /gene="nifJ"
                     /locus_tag="ACIE68_002237"
     CDS             26564..30088
                     /gene="nifJ"
                     /locus_tag="ACIE68_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFX5908548.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLIRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGSWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSVALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         7495 a         8091 c         7413 g         7292 t
ORIGIN      
        1 taaacaaata gttaagagga ataacccaat ataaccaccg cggaaaatgt acagatataa
       61 gcgtacaata ttctttcaat gccagaaaac ttatcagcgc aaaaaacgtc aacgtcatcc
      121 agcgtggacc tgaaatcacc agtgaaaaac aaatgacgat gccccaccag gtacgaatac
      181 gtaatgtaac ttcatcctct tttctctccg gtaaccgcca tagcagaaaa tggtttatca
      241 gcgttgctaa aattaacaac gcgaaaagtg ttgccagaga tttttccagc attatctttc
      301 agcgctcata agaagcacac tccgacaacg attaatcgca gtccagagaa gcagaattga
      361 agcaatgctc cataaaagat tattccactg catccactgt ggataaatgg caacggccag
      421 accccacaga ccaaatatta acgcgcgatc gcttttgcca aatggaccgg cataactgcg
      481 aacaccgtta atcgtctgcg cgagtaaacc gcaaaactcg gtcaatatgg tgcaaaataa
      541 catgagtatc acgagtgatg cgttactttc cggtaaaaat aaaaatggta aatagagcgc
      601 aatatcggaa ataacatcac cagtctcatt caaaattgcc ccaagccgtg tttgctgatt
      661 gcactcacgc gccaacatgc catccagcgc attgagcgcc atacggataa aaagcacgat
      721 gggcaatagc ataaagagga tgggttgtgc caccaacatc aacagcaatc cggtaagaag
      781 agaaagcgcc agtgcagcaa gagtgatgtg attcgctgta acgtggtgct tgtaaagcca
      841 aaacatcgtc ggccttaaca gcgactgaaa gagcggtttt atctggtaga gcgtcattat
      901 caatccttga taattcggtt tgcgctatgg tgcatcaatt gtgatggcgg agatatcaca
      961 acgcggagca tcaacgtaaa ttcggaatat atcaagatgt ggtggatggt tttcctacac
     1021 atctgtttga ttaccgactg gatgtcagtg agataccatc gatcactgta cgaatcaatg
     1081 aatccattgc cttttccctg attatccagc cgcaaatcct ggccttaaac atatgcactc
     1141 catgccatta aaaaagaaac aaaagggaaa tgcatctttc ccttttgtat attcgttatt
     1201 ctcgcgaaat accatccaac gtagacaaca cttcctcaga aagatgcaat ttttcagcag
     1261 ccatattctc ccgtaaatgc gcaacagaag acgtccctgg gatcagcaaa atattcggtg
     1321 aacgttgtaa cagccacgcc agcgccacct gcatgggtgt tgcacccagg ctcgcagcaa
     1381 catccgacag cgtagaggat tgcagcggtg taaagccccc gagcgggaag aacggcacgt
     1441 aggcaatgcc gtcacgagcc aaagcatcaa tcattgcgtc atcagcacga tgagcaatgt
     1501 tgtattcgtt ctgcacacag acgatttcgg caatcttgcg cgcctctgca acctgcgtcg
     1561 gtgtgacgtt gcttaggcca atatgtttta ccaggccttg ttgttgcatc tctgccagca
     1621 cggtcaggct ggcttcaatc gatccttccg ctgggccatg accatccccc atcataatgc
     1681 gcaagttaac cacatccagt acgtccagcc cgagattacg tagattatcg tgcaccgctt
     1741 tttgcagctc tgccggagaa aatgcgggca accaggatgc gtcctctcca cgccgcgcac
     1801 caattttagt gacaattgtc aggtcgtcag agtaaggata aagcgcttcg cggataatct
     1861 gattggtgac gtgcggacca taaaagtcgc tggtatcaat gtgattgacg cccaatgcca
     1921 gcgcctcgcg cagcacggtt atagcgacgt ggcgatctct tgggggacca aaaaccccag
     1981 gacctgccag ttgcatcgcg ccataaccaa gacggttaac ggattttgta ccgagagtaa
     2041 atgtattgct gctcatcgca ccctgccctg taataaaacg gattaagaaa aatcagtata
     2101 gaaacgaaaa gagataaata atgcgagggc gtaaacaaga ttactggatg agttgctcta
     2161 ttgtcctgaa aaacacaaaa caaaacgcgt aaagcgccgc atccggcaat aaaaaaaccg
     2221 caacctgtat cagcgttgcg gtttaggtat cacatgctaa aaattagaat gttcagtatc
     2281 tggacgttac tgtttcggtc gcagattctc atcaaatacc agacgtttcc tgttcggttc
     2341 aacttcccgc aatggctcaa cttgatgcaa cgtctgctta caacgagtca ccagcacctg
     2401 atactcatgc gtaccctgct ttttccatgc cagctcctgc tcactgagtt cacgaatcgc
     2461 tttcgccggg ctgccgacaa tcaggtaatt ggcaggcatt tctgctttgg ctttcacaaa
     2521 tgcggatgcg ccaacaatgc tgttctcgcc aatcaccgcg ccgtccatca ctaccgcgtt
     2581 cattcccact aatgcattgc ggcggataat gcaaccatga aggatagcgc tatgaccaat
     2641 atgtccatct tcttccacga cagtatcctg ctcgggaaaa ccgtgcataa cgcaattatc
     2701 ctgaatgttc gcgccatctt tcaccacgat acgaccaaaa tcgccacgca ggctggcatt
     2761 tggcccaacg taaacgccct tgcctagaat aacatcgccg atcaataccg ctgtcggatg
     2821 gacaaaactc tcttctggca caaccggagt cagaccatct atctggtaaa ttggcatatc
     2881 gcctcctgtt caatattcaa accgccaaaa cgttgaaaat acaggcttcc cggcgcaggc
     2941 agttcaccga ccgaggtttc acctttttca ctaacgaacg ctaaagcagc aggcgctacg
     3001 cgctgataaa tgttgataca cagctgacgc gccgtatgcc ctgcccagtg cgccggaagc
     3061 aactcctctg gcaacaatgg gtctttaagg acgacacggc gataaaaatg gatcagtaaa
     3121 agctgaatat gaaatgcacg ctccggggtt aactcgtctg ctgcctcttt taacagcggc
     3181 accagcgggc ggaatgactg aataaaggtt tcgtacatgg cattttgttc agttaaatgc
     3241 cagcactctt ccactctggc acgcagtgct gcacgagaaa gcgccagtgg tatttgcgct
     3301 tcaaaacaaa tcacgttatc caccaccccc gcttcatgca aaagtgtctg tacatccgcc
     3361 agtttttgcg acggcgatgc catcaggctg ggtgccagtg cgccaaaacc ttgccagatc
     3421 aactgctttt tgacatcagc cagcgttgat ttatctaaac cttccgagag caacaggagc
     3481 catttaccat cccatgcagg ttgctctgcg cgataaattt tactttctgc ccgtcgcgtc
     3541 aggcgcaagc ctttatcact gaggctatag aaactgcgtc gcccgatgcg ggaaacatcc
     3601 agccagcctt ctttattaag acgaaacaaa gcggtgcgca cgaaacgctc accaaatccc
     3661 agcccttcca gcaaagcagc cagactaccc agccagattt caccaccacg atgggaaagc
     3721 gaatcaccat acagagagga gatcaaagat gtgccactga ccggaacagc gtttacagca
     3781 tgttggataa aagtaacaag tttactcatt cgattcagtc tgttgttatt gttgtgattt
     3841 atgaatcata gcacaggtcg tatcgacttc tggccctggc gctttacctc accagggcca
     3901 gacgacacgt caggcaccaa caatattgcg cagatcaaac acccgacacg ccttgccttc
     3961 tgaacgcggg atactgccac agttaacgat catcacatcg gtagagatcc ccaccatcga
     4021 cttaatccga tggcgcagtt ggtggcacac ctggcaacgc tgctcatgcg tcaatgtcag
     4081 gctgctttct ttcaactcca ctttcacaga aagtgaatca agatgccccc ggcggttcac
     4141 ctccagttgg taatggggcg ataaatgttc gaatttgaca atctcttctt ccagttgtga
     4201 cgggaagaca tttacaccgc gaatgatcaa catgtcatcg ctgcgtccgc tgatgcgatc
     4261 catgcggcgc atagtccgcg ccgttcctgg taacagtcgg gttaaatcac gcgtgcggta
     4321 acgaatgacc ggcaatgctt ctttggtcag cgtggtgaat aacagttcgc catgttcacc
     4381 atcggcaagc ggtgtgccgt catgaggatt aacaatttca ggatagaaat gatcttccca
     4441 aatggttggg ccgtcggtag tttccagaca ctccatcgcc acccccggcc ccatcacttc
     4501 tgacaggcca taaatatcca gtgcggtgat ccccagacgc cgttcaatct ctttacgcat
     4561 ggcctgtgtc cacggctcag caccaaacac tccaacccgc agcgagcaac cgctggcatc
     4621 accgcccaac tgccgctcca gctcttcaat caggttaagg caataagatg gcgtaaccat
     4681 gatcatatct ggttgaaaat cgcggatcag ttgcgcttgt ttttccgtct gcccaccaga
     4741 catcgggatc accgtagcgc ccagacgttc ggcaccatag tgcgcgccca gcccaccagt
     4801 aaacaggccg taaccatagg caacatgaat tttgtctttg ggcgtgccac ctgccgcacg
     4861 caaggaacgc gctacaatat tggcccagtt atcaatatcg ttttgcgtat agccgacaac
     4921 tgtcggttta cctgtggttc ccgaagaagc atgaatgcgc accacttgtt ccatcggcac
     4981 ggcaaaggtg tcgaagggat agttgtcgcg cagatcctgt ttggtggtac agggaaattt
     5041 acgcagatct gaaagttccc tgaaatcatc aggatgtacg cctgctgcgt caaatttgcg
     5101 ccgatacatc gggacatttt catacgcgtg tttgagcgtc catttcagac gttgtgtttg
     5161 caacgcctgt aactcatcaa cagacgcggt ttcaatcggg tcaagctttg tattggttat
     5221 cattgtaggg tactcgcagg ttgatatgct caaacacgct ccagaatcat ggcgatgccc
     5281 tgaccgacac cgatgcacat ggtgcacaat gcgtaacgac cgttacgccg atgcagctca
     5341 tggctggcag ccagtgccag gcgggcacca ctcattccca acggatggcc taaggcgata
     5401 gcgcctccgt tggggttaac atgtggggca tcatcaggca gccccaattc gcgtagtaca
     5461 cccaacgcct gggccgcgaa cgcttcgttc agttcaatca cgtccatatc gtgaatactc
     5521 agccccgcgc gttccagcac ccggcgagtt gcaggcaccg gaccaagccc catcaggcgc
     5581 ggttccaccc cggcggttgc catggctacg attcgcgccc gcggtgtcag tccttgcgct
     5641 gctgccatct gttcactggc aataatcaac gcagcggctc cgtcattcac cccggaggca
     5701 ttgcctgcgg taatcacccc attggcacga aatggtgctt ttaacccacg taactgttcc
     5761 agcgtcgttt ccgggcgcag atgctcatca tgttgtattt ctgttacaac gcctttcttg
     5821 tttttcaaca caaccggaac aatctcctca gccagaatgc ctgaggattg cgcttttgcc
     5881 gtacgttgct gactgcgtag cgcaaaacta tcttgatctt ctcgcgatat ttttaacagt
     5941 tcagctacat tctctgccgt ttccggcatg ctgtcagttc caaattgttg agccatgagc
     6001 gggttcacaa atcgccagcc aatagtggta tcaaacatct cagcctgacg agaaaatgca
     6061 ctggttgcct tgcccataac aaacggtgcc cgagacattg actccacgcc accagcgatc
     6121 agcaaatcgc catcgcccgc tttaatcgcc cgtgcggcga accccagtgc gtccagcccg
     6181 gaaccacaca agcggttaat ggttgtgccg gaaacactct gcggcagccc cgccagtaac
     6241 gtcgccatcc gggctacgtt acggttgtct tctcccgcct gattagcaca gccgaggatc
     6301 acatcatcga tacactccgc atcgagacgc gggtttcgca ccagcagctc ccgcaaaggg
     6361 atagcagcca gatcgtcagc ccgaacacct gataatgccc caccatagcg gccaattggc
     6421 gtacgaattc cgtcacagat aaaggcttca cgcatcaggc ttctcctgta atggtgccgc
     6481 cgatgcggtg agatttaccg cgaaacagcg caaccgtttt ttgttgttgg ttaacaattt
     6541 cgatgtcgta aacaccggtt tgcttgccct gatgacgtac ctgcgcagta gcagttaagg
     6601 tgtctccggc aaagcctgga cgtaaaaaat caatcgtgca ggcagaagcg acggctgcca
     6661 gcccctggct attgcaggcg taggcaaagg cagtatcagc cagtgaaaat agctgcccgc
     6721 cgtggcaact ttgatgaccg ttaagcattt gtgcagtgac ggtcatggtc actacagcaa
     6781 agccttcatc cattgagata atgtcgatgc caagcgcttt ggcgcaggca tcgttctcat
     6841 acattgcatg ggcattttgc caggccttat gactcataac cgctctccag aagcgcccgt
     6901 tggcgcagca atgaacatgg gcgatagcgt tcttcgccat agtgatgctg tagattttca
     6961 aggagcctta atattcgctg ccagccaagt tgcgctcccc aggcaagtgg gccatatgga
     7021 taattcaccc caagacgcat ggcggtatcg atatcctgtt cagaggccac gcctttttga
     7081 agcgcatcaa gggcttcatt gatgatcatt gctaccgttc gccaaatcag cattcctggg
     7141 taatctgcaa tttgcagcac tgttttgccc tgctgttgca ggtaataaat ggccttgcgg
     7201 gtcgctgagt ccgggttcac cgctgccgca gcaatggtca ccaccttgcc cgccattttg
     7261 tcgaccacta ccaccgggcg tgccagtcgt attgccagcg cctgtgccgt ctcgccttgt
     7321 gtctcaatca ataaaacatc gtcaatttcc gtgacaccgt cacttttctt ttctactttc
     7381 attgggctaa aactgtcgct taccgcttcc aggccaacaa ctgcctcacg ttccgcgcgc
     7441 cagtcgtaca cgcccagccc acttttcttg cccaaccgtc caccaatcac cagttcctgt
     7501 tgcaccagcg aaggtaaaaa acgacgctcc tgccagaaag cgttaaacac cgaacaggtg
     7561 acagcaaaat tgacgtcctg accaatcaga tcggttaatt ccagcggccc catcgggaaa
     7621 ccagcgccat cgcgaagtgc agcgtcaatc acttctggtg cagcaacctg ctcttccagt
     7681 gcccgccagg cctcggaata ataaggacgc gcaacacggt taacgataaa tccgggggtc
     7741 gaatgacagc gcactggctg cttaccccaa ctcaatgtca gttcacacaa ctgctcgaca
     7801 acttccgctg ccgttgccag cccactgacc acctccacca acttcatcac cggtgccggg
     7861 ttaaaaaaat gcagccccgc aacacgttca ggatttttta cctccgcagc aatcgcggtt
     7921 atagagattg acgaagtgtt agtggtcaat agcgtttgtg gcgggcaaac ttccgccagt
     7981 tgtgcaaaga gcgctttttt aacttccaga cgttcagacg ccgcttcaat aaccagattc
     8041 gctgctgcca gcgcgtgaat atcggtcacc gggatcaggc gtttcaatgt gcgttcacag
     8101 gtttcagcgg tcagttttcc ccgcgtcacg cgtgaattta gccgcgcgtg tatcccgtcg
     8161 attgcgcggg tcagcgcttc agcagaaatg tcatacagta aaacctgatg tccatgactg
     8221 gcggcaactt cagcaatgcc tgcccccatg gtgccgctcc caatcactgc cacagtttgc
     8281 acatttatca tcatgctatt tccccgtgaa ctgcggtgag cgtttagcca ggaacgcact
     8341 gacaccttca cgataatctg cgctccgtcc ggcaagtcgc tgatagtcac gttccagatc
     8401 cagttgcgta tcgagcgtat tggtttcagc gctatttatc gcttgcttga taagtccaag
     8461 accaaatgtc ggttgtgtcg ccagatgccg tgccagctgt tgcgcggtat ctgccagcgt
     8521 ttcatcatca acaacctgcc agatcatccc ccattcgtgc gcctgttcag cactcagttg
     8581 attccccagc agtgccagcc ccatggcgcg cgctcgtccg gcaacgcgtg gcagtaacca
     8641 ggttccaccg caatcgggta ttaagcctaa cttactaaac gccatgacga attttgctga
     8701 acgggcagca ataacgatgt cgcaccccag tgccagtgtt gcgcctgccc ctgccgccac
     8761 gccattgact gcacagatca ccggttttgg cagttttgcc aggcgacgta ccagtgggtt
     8821 atagaaacgt tcaactgaca tccctaaatc cggtgcgggg cctgtgggat caacgttacg
     8881 atcgttaaga tcctgaccag cacaaaatcc gcgcccggca cctgtaagta acaggcaacg
     8941 gatagtgtcg tcgcgctcga cctgtttcag gcactctgcc agttgtgcgt gcatctcatc
     9001 attaaaactg ttcaggcgtt ccgggcggtt gagcgttagt gtcatcacgc ccttttctac
     9061 atgactgagg atgaattcca tcattagcgt cctttaaagt cgggcgtgcg tttttgtaag
     9121 aaagcggaga tgccttcatg acgatcttct gttgccgcca gcaaggtgaa taactgtcgc
     9181 tcctgggcaa gtccggcttg caaagccact tcctgcgact ggcgcagcgc ttgctttgcc
     9241 gcttgtaagg ccagcggcga gtgacgtgcc attttcgatg ccagctgtaa ggcgtattcg
     9301 agggttaaat cgctggggaa gacgtcgcta accagcccgg cctgctgtgc ttgctgggcg
     9361 gtgatacttt ctccgctcag caccattttg ctggctaacg atttaccgac actacggatt
     9421 aaacgttgcg ttccgcctgc gccaggcatg atgccgagag tgatttccgg caacccaaaa
     9481 cgcgcgttct ctccggcaac caccacatcg cacaacaatg ccagttcgca acccgcccca
     9541 agcgcgtaac cattgacggc tgcgataagt ggtttattaa aggcctgcaa tcgcgcccat
     9601 agctgcggac gtgtatcgtt taaggtggcc gcgagatctt tttctgccat ttcgttgaga
     9661 tcggccccag cggcaaaaaa gcgtgcatta ccggtaatca cacagaccga aatgctggta
     9721 tcggtagccg cagcttccag ttcatttacc agttgcatca gcagggcatt atttagcgca
     9781 ttacgtgcgg cgggacggtt aagggtcagc aacaatactc gttgctgacg gctgacgatc
     9841 agttcgctca tgccatcccc ttcgcgtcaa agtcaaccac cacatcactg gtcagcggca
     9901 gtgcctggca actcaacaca tagcctgcag ccagttcatc cggttccaga ctgtaattgg
     9961 tttccatcgc cactttgcca cgcagcactt tgcatttgca ggtcgcacag acgccgcctt
    10021 tgcaggcata gggcagatca gccccctggc gcaatgccgc atcgagaatg ctttcatcgt
    10081 cggcattaag cacgatttcc cgatcccgcc catcctgacg tacagtcact ttttgtccgt
    10141 cactttgcac gttaacgcta cgtttgacgc gcgtgccagg cgtattaaac cgctccagat
    10201 gaatggtttt atctggcatt cccagtgctt ttaaggcggc ttccgtctca tccatcatcg
    10261 ccgccggacc acaaataaat gcctcatcat aaagacgaaa attaatgagc gaggccccaa
    10321 gtgactgtaa tttttcaccg tcaatacgcc cgtgaagcag atcgctgtcg agggtttcct
    10381 gactgaaaat gcacaacaac tgtaaacgct gaggatattt gtctttcagg tctgccagtg
    10441 cctggcgaaa catcatgctc tggctggtac ggttaccgta gatcagggtg aactgacttt
    10501 caggctcggt ttgtaaagtg gcggcgataa tcgccagcat tggcgtaata cctgatcctg
    10561 ctgcaatagc cagatagcgc ccctggcgtt cggcctgcgg ctgatagccg aaatggccct
    10621 gcggcaccat gacttccagc gtcatcccct gacggatgtg atcgcgggca tagcgggaga
    10681 aacgtccgcc ttcaatggct tttaccgcca cactaatttc gccaggcagg tagctgcggc
    10741 aaatggagta acaacggcgt aattcttcac catcaaggct ggcttttaag gtcaagtgtt
    10801 gaccggggcg aaagcgatac gcctcctgca aaggctgggg caccgcaaag gtaatggtca
    10861 ccgcatcacg ggtttccggc tccacttttg ccaccgttaa ggaatgaaac gttgtcatgg
    10921 catcctcaaa tacatttgaa ataatcgaaa ggttcgcggc aactatcgca gcgatacaat
    10981 gctttgcagg ccgtggaacc aaattcactg ataagtgtgg tatggacgct ggcacagcgc
    11041 gggcaacata cttctggcgg caaatgggca tggcaactgt gtccggcggg cgggctaatg
    11101 ccatactgtc gcagacgctc acgggcatcg ggggtcatcc agtcggtggt ccatgccggg
    11161 tcaagttgca gcacaacctg aacgggggta aacccgtggg ttgacattgc ctcacgtatc
    11221 gcgccaatca aatgttccgt tgccggacaa ccggaatatg tcggcgtaaa gccgatcacc
    11281 catccttctc ccatctgtgt cacattacgc accatgccta aatcagtaat ggtcagcacc
    11341 gggatctccg ggtccgggat ctggctgagt agcgcccata tctcatgaac ctgtggcggt
    11401 gcaatggtgg ccagacgttg catacccatc tcctctgtta ccattgctga ccgggcaaga
    11461 cacgctggag atactgcatt tctgccagca tgggtccaag gtgttcggta tgcagtcctt
    11521 ttttaccgcc agtgcgatac gcctgctctt gcggtacgtt caatgtggct tcgttgatcc
    11581 cagcaaaaac ttcggcttcc cacgctgcgc gtaaagtgcg tggatcaacc gcaataccct
    11641 cttcactcag tgcaatatca atctcgtcgg catcgaacag ttcggcggta aaacgccaca
    11701 acttgttgat ggcctgctgc atcttttgcc ctgatacgtc agtaccattg cccagtcgct
    11761 ccagccagcc acgactaaaa cgcaggtgat aacgcgcttc tttaattgct ttggcagaaa
    11821 tcgccgccag ttgcggatca cggctctcca tcagacgggt aaagagcgcc acatgccatg
    11881 catcgatgaa atactggcgt gcaatggtgt cggcaaaatt gccgtttggc tgttcaacca
    11941 acaataagtt gctgaactgg cgctcgtctc gggtgaaggc cagggtatct tcatcgcctt
    12001 ctcccgctaa ttcagcggca tacgataaga agttgcgtgc ctggcctaat aaatcgagtc
    12061 caatatttgc cagtgcgaga tcgatttcca gttccggtgc gtgaccgcac cattccccca
    12121 accgctggga gagcaccaga cagttatcgc ccaggcgcaa ggtgtaagcc gttaactgat
    12181 tcatttccga cctcacatgt gctcaatgcc atcagggatg gtgtaaaacg ttggatggcg
    12241 atagaccttg ctttcagccg gatcaaaaaa ttcaccgcgt tcttccggtt gcgaggcaac
    12301 aatttcactc gccttcacca cccaaattga acatccttcg ctacgacggg tgtaagcatc
    12361 acgggcattt tccagtgcca tccgctcatc ggcagcatgt aaactgccaa catggcggtg
    12421 tgataagccc tgtttgccac gcacgaaaac ttcgtataac ggccagtaaa cattactcat
    12481 tttgaatctc cttatgcgac cttacgggca agttgttttt gtgcatgggc cagcgctgct
    12541 tcccgtaccc aggtaccttc ttcccaggct ttacgtttgg cgtcgagcct ctcctgatta
    12601 caaattccgc gaccgttaat tacttcgtta aactcctgcc agtcgatctc accaaagcgg
    12661 tagtgaccgc tttcagtgtc aaaatgcaga tccgggtcag gaacggtcat accgagcatt
    12721 tcaacctgtg gaacggtgtt gtccacgaaa cgctggcgga gttcgtcgtt ggtgaaacgt
    12781 ttgattttcc aggcgagact tctggcgctg tttggcgagt tatcatcgtt tggcccgaac
    12841 atcattaagg ctggccacca gaaacggtta atggcgtctt gcaacatctg cttttgagct
    12901 tcgctgccct gcgccagtgc catgcaggct tcaaacccct gacgctggtg aaaactctct
    12961 tctttgcaga ttttcaccat tgctctggca tacgggccat aagaagtgcg gcataacgcc
    13021 acctgattga cgatcgctgc gccatccacc agccagccga taacaccgat atcagcccaa
    13081 ctcaatgtcg gataattaaa gatggaggaa tatttcatcc gtccgtcgag cattttttgg
    13141 tagatgtctt ctctggcgca gcccagtgtt tcggcggcgc tatagagata cagaccatga
    13201 ccggcttcat cctgcacttt ggccaacaga atggctttac gccgcaaggt tggtgcgcga
    13261 gtgatccagt taccttcagg caacatacca acaatctcgg agtgcgcatg ctgcccaatc
    13321 tggcggatca gtgtctttcg gtaagcatcg ggcatccagt cctgcggctc gatagccgtt
    13381 tcctgggcta tccgttgctc aaagcgttct tcttgggtca cgtcatcacc tttacgattc
    13441 cagttttcgt gaattaatta aaaacacgaa tcacgttgtt ttatgaaagt tacacaatgg
    13501 ttaactaaaa tcacaaattt tgtatgtttg aactgtgacg gatttcgcac cctattttta
    13561 taactcatta tatatcagtg cattaatgtt tttagtggtt gatatacaga tcgcatagtt
    13621 aacatttcgt taaaatacgc cttgcttttt atgattcgtg atttaactat tagcaacaga
    13681 aatgtgaaac atctggagag tagcgatgca gcagttagcc agtttcttat ccggtacctg
    13741 gcagtctggc cggggccgta gccgtttgat tcatcacgct attagcggcg aggcgttatg
    13801 ggaagtgacc agtgaaggtc ttgatatggc ggctgcccgc ctgtttgcca ttgaaaaagg
    13861 tgccccagcc cttcgcgcta tgacctttat cgaacgtgcg gcgatgctta aagcggtcgc
    13921 taaacatctg ctgagtgaaa aagagcgctt ctatgctctt tctgcgcaaa caggcgcaac
    13981 gcgggcagac agttgggttg atattgaagg tggcattggg acgttattta cttacgccag
    14041 cctcggtagc cgggagctgc ctgacgatac gctgtggccg gaagatgaat tgatcccctt
    14101 atcgaaagaa ggtggatttg ccgcgcgcca tgtactgacc tcaaagtcag gcgtggcagt
    14161 gcatattaac gcctttaact tcccctgctg gggaatgctg gaaaagctgg caccaacgtg
    14221 gctgggcgga atgccagcca tcatcaaacc agctaccgcg acggcccaac tgactcaggc
    14281 gatggtgaaa tcaattgtcg atagtggtct tgttcccgaa ggcgcaatta gtctgatctg
    14341 cggtagtgct ggcgacttgt tggatcatct ggacagccag gatgtggtga ctttcacggg
    14401 gtcagcggcg accggacaga tgctgcgagt tcagccaaat atcgtcgcca aatctatccc
    14461 cttcactatg gaagctgatt ccctgaactg ctgcgtactg ggcgaagatg tcaccccgga
    14521 tcaaccggag tttgcgctgt ttattcgtga agttgtgcgt gagatgacca caaaagccgg
    14581 gcaaaaatgt acggcaatcc ggcggattat tgtgccgcag gcattggtta atgctgtcag
    14641 tgatgctctg gttgcgcgat tacagaaagt cgtggtcggt gatcctgctc aggaaggcgt
    14701 gaaaatgggc gcactggtaa atgctgagca gcgtgccgat gtgcaggaaa aagtgaacat
    14761 attgctggct gcaggatgcg agattcgcct cggtggtcag gcggatttat ctgctgcggg
    14821 tgccttcttc ccgccaacct tattgtactg tccgcagccg gatgaagcac cggcggtaca
    14881 tgcaacagaa gcctttggcc ctgtcgcaac gctgatgcca gcacaaaacc agcgacatgc
    14941 tctgcaactg gcttgtgcag gcggcggtag ccttgcggga acgctggtga cggctgatcc
    15001 gcaaattgcg cgtcagttta ttgcagacgc ggcacgtacg catgggcgaa ttcagatcct
    15061 caatgaagag tcggcaaaag aatccaccgg gcatggctcc ccactgccac aactggtaca
    15121 tggtgggcct ggtcgcgcag gaggcggtga agaattaggt ggtttacgag cggtgaaaca
    15181 ttacatgcag cgaaccgctg ttcagggtag tccgacgatg cttgccgcta tcagtaaaca
    15241 gtgggtgcgc ggtgcgaaag tcgaagaaga tcgtattcat ccgttccgca aatattttga
    15301 ggagctgcaa ccaggcgaca gcctgttgac tccccgccgc acaatgacag aggccgatat
    15361 tgttaacttt gcttgcctca gcggcgatca tttctatgca catatggata aaattgctgc
    15421 tgccgaatct atttttggtg agcgtgtggt acatggatat tttgtgcttt ctgcggctgc
    15481 gggtctgttt gtcgatgccg gtgtcggtcc ggtcatagct aactacgggc tggaaaactt
    15541 gcgttttatc gaacccgtaa aaccaggcga taccatccag gtgcgtctca cctgtaagcg
    15601 caagacgctg aaaaaacagc gtagcgcaga agaaaaacca acaggtgtgg tggaatgggc
    15661 ggtagaggta ttcaatcagc atcaaacccc ggtggcgctg tattcaattc tgacgctggt
    15721 ggccaggcag cacggtgatt ttgtcgatta atcggtgaat gaagggcaac ggcgaatagt
    15781 tgccctttta tttcactaaa ttttgtgacg ttgtcacatt atgcatgatg tgtacatcta
    15841 ttttcagggc atccactgta tgaaaagctg gcacacctgc caaacaacct ggcaggtgca
    15901 ggcaatcccc tttgcatcag tactgataat gtgaacctga ctaaaccgcc cacagagcgc
    15961 ggttgctaac aagaacacaa catctgacga ggttaataat gggaagcccc tctctgtatt
    16021 ctgcccgtaa aacaaccctg gcgttggcag tcgccttaag tttcgcctgg caagcgccgg
    16081 tatttgccca cggtggtgaa gcgcatatgg tgccaatgga taaaacgctt aaagaatttg
    16141 gtgccgatgt gcagcgggac gactacgccc agctctttac cctgattaaa gatggcgcgt
    16201 acgtgaaagt gaagcctggt gcgcaaacag caattgttaa tggtcagcct ctggcactgc
    16261 aagtaccggt agtgatgaaa gacaataaag cctgggtttc tgacaccttt attaacgatg
    16321 ttttccagtc cgggctggat caaacctttc aggtagaaaa gcgccctcac ccacttaatg
    16381 cgctaactgc ggacgaaatt aaacaggccg ttgaaattgt taaagcttcc gcggacttca
    16441 aacccaatac ccgttttact gagatctccc tgctaccgcc agataaagaa gctgtctggg
    16501 cgtttgcgct ggaaaacaaa ccggttgacc agccgcgcaa agccgacgtc attatgctcg
    16561 acggcaaaca tatcatcgaa gcggtggtgg atctgcaaaa caacaaactg ctctcctggc
    16621 aacccattaa agacgcccac ggtatggtgt tgctggatga tttcgccagt gtgcagaaca
    16681 ttattaacaa cagtgaagaa tttgccgctg ccgtgaagaa acgcggtatt actgatgcga
    16741 aaaaagtgat taccacgccg ctgaccgtag gttatttcga tggtaaagat ggcctgaaac
    16801 aagatgcccg gttgctcaaa gtcatcagct atcttgatgt cggtgatggc aactactggg
    16861 cacatcccat cgaaaacctg gtggcggtcg ttgatttaga acagaaaaaa atcgttaaga
    16921 ttgaagaagg tccggtagtt ccggtgccaa tgaccgcacg cccatttgat agccgtgacc
    16981 gcgttgctcc ggcagttaag cctatgcaaa tcattgaggc tgaaggtaaa aattacacca
    17041 ttactggcga tatgattcac tggcggaact gggattttca cctcagcatg aactctcgcg
    17101 tcgggccgat gatctccacc gtgacttata acgacaatgg caccaaacgc aaagtcatgt
    17161 acgaaggttc tctcggcggc atgattgtgc cttacggtga tcctgatatt ggctggtact
    17221 ttaaagcgta tctggactct ggtgactacg gtatgggcac gctaacctca ccaattgctc
    17281 gtggtaaaga tgccccgtct aacgcagtgc tccttaatga aaccatcgcc gactacactg
    17341 gcgtgccgat ggagatccct cgcgctatcg cggtatttga acgttatgcc gggccggagt
    17401 ataagcatca ggaaatgggc cagcccaacg tcagtaccga acgccgggag ttagtggtgc
    17461 gctggatcag tacagtgggt aactatgact acatttttga ctggatcttc catgaaaacg
    17521 gcactattgg cattgatgcc ggtgctacgg gcatcgaagc ggtgaaaggt gttaaagcga
    17581 aaaccatgca cgatgagacg gcgaaagatg acacgcgcta cggcacgctt atcgatcaca
    17641 atatcgtggg tactacacac caacatattt ataatttccg cctcgatctg gatgtagatg
    17701 gcgagaataa cagcctggtg gcgatggacc cagtggtaaa accgaatact gccggtggcc
    17761 cacgcaccag taccatgcaa gttaatcagt acaacatcgg caatgaacag gatgccgcgc
    17821 agaagtttga tccgggcacg attcgtctgt tgagtaaccc gaacaaagag aaccgcatgg
    17881 gcaatccagt ttcctatcaa attattcctt atgcaggtgg tactcacccg gtagcaaaag
    17941 gtgcccagtt cgcgccggac gagtggatct atcatcgttt aagctttatg gacaagcagc
    18001 tttgggtaac gcgttatcat cctagcgagc gtttcccgga aggcaaatat ccgaaccgtt
    18061 ctactcatga caccggtctt ggacaataca gtaaagataa cgagtcgctg gacaacaccg
    18121 acgccgttgt ctggatgacc accggcacca cacatgtggc ccgcgccgaa gagtggccga
    18181 ttatgccgac cgaatgggta catactctgc ttaaaccatg gaacttcttt gacgaaacgc
    18241 caacgctagg ggcgctgaag aaagataagt gattgtttca gacaaaaaaa cggcaccagg
    18301 tgccgttttt tacttatgag cgaaccagat taataccgta cacacaccga cttagtttca
    18361 caccaaccgt ccagccagtc ggggccaaaa tcacggcccg ttcctgactg cttcatccca
    18421 ccaaacggta agttagcgtc aattaaggta tggctgttta cccacaccgt ccctgcctgt
    18481 aagcgatcgc tatattccag agcctgggag agattttgcg tccagacact ggcagttaag
    18541 ccatattccg tgtcgtttgc cagttgtaac gcctcttctc catccgctac tcgcaccagg
    18601 tttaccaccg gaccaaacac ctcttcacga gttaagcgca atttagcatc gggatttacc
    18661 accagcgttg gcgcaacata atacccctct ccggctggtc cattcgaccc gcgaatcagc
    18721 tctgcttgct gtgcctgcgc atcgtcgagg aatgaacaca ctttgtcgca gtgcgcacga
    18781 gaaaccaaag ggttaatctg tgcaacaggt gacatccccg gtcccacttg caacgatttt
    18841 accgcctgct caaatccact aaccagcgtg tcaaacaacg gcgcttcaat ataaattcgc
    18901 gaactggcgg cgcatacttg cccttgattc aggaagcttc cggtcatcaa gccttcaata
    18961 acccattgcg gatcagcatc ttttaataca attgccgggt ttttaccgcc cagttccagc
    19021 gttacacgcg ttaagtgatc agcagcagtt ctggcaatac cttttcccgt cgcggttgaa
    19081 ccggtaaaac tgattttcgc aacatgagga tgtgacgtca gggccgcgcc gcatacagca
    19141 cctgacccgg tgacgacatt aaaaacgcca tcagggatac cagcctcgct ggccagttcc
    19201 gccacgcgca acatcgtcag tggcgtggtt tccgaaggct taatcacgat tgaacagcct
    19261 gctgccagtg ctggcatcac cttccacata ccaatcatca acggaaagtt ccatggcaca
    19321 attcccgcca ctacgccaac cggctcttta cgcgtccagg cctgataacg cgccccctgg
    19381 ggtaagggaa tcgacaagtc cagcgtttta cccgcgattt tggtcgttaa cccggcggta
    19441 taacgcatcc agttcagcgt acagcccact tcaaaagcac gggaaatggc aattgacttg
    19501 ccttgctcca gggtttccag ttgcgccagc tcctcactgt gctgttccac cagatcggca
    19561 aaacgtagca gaatacgttc acgctctgcg ggtaatcgcc cggcccagcg acgcgagaca
    19621 aaggcccgcc aggcagacat gactgcgtta tctacatccg cttcgttggc atcagcagta
    19681 gacgcaattt cttgcccggt ggccggatca aagatcgcca accgtttttc actttgtgcg
    19741 gggccaggac gaccatcaat ataaagaccg tgttgacgat cgagaaactg ttggacctgg
    19801 cttaatactg ctacatgcgg ctctgtcatc acttttcctt attatttacc cagtgtgatt
    19861 tcagtgtacg cagcgacaag aaaagtcact tttttcgctg tgacattcgc tttgtcagct
    19921 gtgacaagct ccgcaaatcg tgacaataac cgcttatgtc tctatgcctg acaagactta
    19981 tttcctatag tcaggctcat tagcttcgtt tattgcaaca caaatgcaac aataaaaata
    20041 catttcacag agcgaaaacg tgccatgaac cccgcaatgg ataatgagtt tcagcaatgg
    20101 ctttcccaaa tcaatcaggt atgcggaaat tttaccggac gcctgctgac tgagcgttac
    20161 acgggtgtac tggacacgca ttttgccaaa ggactaaagc tgagtaccgt gacaaccagc
    20221 ggggtgaatt tatcccgcac ctggcaggag gtaaaaggca gcgacgatgc ctggttttac
    20281 accgtttttc agcttagtgg tcaggcaata atggagcagg atgagcgtca ggtgcagatt
    20341 ggcgctggcg atattacgtt actcgatgcc tcacgcccct gttcgcttta ctggcaggag
    20401 tcttctaaac agatttcatt acttttgcca cgcactctgc tggaacaata ttttccccat
    20461 caaaaacctg tctgcgcaga aagactggac gctgacttac ccatggtgca actcagtcat
    20521 cgcctgttac aggagagcat gaataatccg gcactttctg aaacagaaag tgaagctgcg
    20581 ctacaggcga tggtgtgtct gctgcgcccg gtacttcatc agcgggaatc tgttcaacct
    20641 cgtcgtgaac gtcagtttca aaaagtggtt acgttgatag acgataatat tcgcgaagag
    20701 atattacgcc cggagtggat agccggagag acaggtatgt cagtacgtag tttgtaccga
    20761 atgtttgccg ataaaggttt ggtggtcgcg caatatattc gtaaccgtcg tctcgatttt
    20821 tgtgcagatg cgattcgcca tgccgcagat gatgaaaaac tggcaggcat cggctttcat
    20881 tggggatttt ctgaccagag tcatttttca acggtattta agcaacgctt tgggatgacg
    20941 ccaggcgagt atcgacgtaa attccgctaa ctaattggcg ttgtgtgtac tgcaacgcca
    21001 attagttaaa acagatattt gactatgcat ttttcagggc gaaaggcaat ttgacaggag
    21061 tttttgaata ttcaccttgt ttcaggaaaa tagatcaatc gctttctcct gtagcgttat
    21121 cccgatattg cggttttgct attaaaattt tcgcacccat ttaccgttta tcccttgtac
    21181 atattccccc ggtttggccc gagctacgag cttttgcccg gcgagtttcg cgatatcatc
    21241 tacagacaca ttattttgct ttgccagttg ttggtaactc gctttacgtt cggcattaat
    21301 gtcggttact aatttctctg tttcagcatc cgttttcagc gcaaccagat aaccataaaa
    21361 tgtttcacct acccgcccct gagttctggc ttcatccaga gtcaatgcca ttacattgct
    21421 gcttaccagc ccaacaagaa acgcacaaag aagtaatgtt ttcttcatca tcgcctcaga
    21481 aaagatcgct acgagtttca agcagctctt cgacatcttt gtctgccttg atgatgatct
    21541 catgctcaat tttaacgttc atattgatag tgatcggttc cttgggcgca gcgacttcaa
    21601 tacgcggcgt acaggcagcc agcataaaag atgacgtcaa cgcagccagt aaaattttca
    21661 ttgtttttcc tcacactctt ttccttgcgg acagtcattt cccggcagac gtgcgttctg
    21721 ctccaaccat gcctggagat tatcgccaaa gcgtaaactg cgccacagcg taaaaatatt
    21781 ctcttcatga tggtaattaa ggtttaccgt accgctttta ccatcaacgc gactggtacc
    21841 tgtaatgttg gcctgcatgg ttaataaacc gagattatct aaattaatat ttgtcgatga
    21901 acggctaatt tccatatagc gcaaccagtt aattgctgaa cccgcagtca tattgtcttt
    21961 caccaccgca tctgcggtgt ctttatccag ccgcaaggtc attggcccgc tatttgccag
    22021 ccaaccatct ttcactatcc atttttcgtt gtttagccat aacggcaatg caccactaaa
    22081 tgccccggac atggcgaatt gtttcggatt gacggcgcta accagttcgc tggatgagag
    22141 attattcagc cgcaacagcg ccgggtcatg ttgcggcata cgtaattgtt tcatcagtac
    22201 gttaccgcct aacacatcga cgctaacatc agtcaacagc aagggttctt cttcagtcca
    22261 cggataacgc ccttgcaaat cagccgtaat atttttcgct gtcaccagat taatcacttc
    22321 ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc caggctccat cggcaaaacg
    22381 gaaaggcagg acaaaatcga caccgttaac ctgattatct ggcatccagg cactaccgcc
    22441 tttcaacacg ccgtgtcctc ccgcgcggaa tccttgttca ggcgcagcag aaaatgcaac
    22501 ctgagcatac agttcaccat cgcgtaagtt catcttccag tcgggtggca ccagcggctg
    22561 gaatacagtc agtgattgtt ttggccacca ggcgttaccg cgcagacgaa taccgtccca
    22621 gcgaccattt acccgaaccg ggccaatctc accagcatgt aaatcgcctt taaagaggaa
    22681 atacgtcgga tcgcgcccat cgacgctaaa ttttaaggtt gatggcggta acacactgcc
    22741 gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg ccgctgaagg agggatgctg
    22801 tgcgtcacgt acccagacga cgggcttgtc gagaattaat cgcggctttt ctaccgtcat
    22861 cgtaccgtat tgtaactggt cgaaaccggt ggagaggtcg gtcagcgtaa tcgtgctgtc
    22921 atgccattca ccttttcctg cgacatccca ggtggcattc atcggtgtaa aactgccttt
    22981 tccccagtag cgccactgcc agcggcccgc gtcagggaga aaatcattcg ccagcccatc
    23041 catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc tgcaagatgg cctgcaaacg
    23101 tccgtcaaca ccacgttggg tgacttttac acccgctaaa ggccagcgga tttcatcgat
    23161 gtccagcgaa tcgatgactc ttcccttcga acgtaataac gcgcctggct caaaggccaa
    23221 cgttgggtca gtcagacttc cgcttagctg cgcaggtaaa cgggcatata aaatgaggtc
    23281 cgcctgtttc gcttcaccgg tcagttgcag aggcagctga ctgttatcca tgcttaattt
    23341 tcctgggcca aaattaagca ccgcgttgcc tttacccgct tgcccctggg tcagcacact
    23401 cagtcgtccg ctgaccagag cgttctcgag ccctgcctgc cagttgtcga ctttgacacc
    23461 cagtcggcca ctcaaaggaa aacctgcata cggccagctc cagcgaccat cgcttacggt
    23521 caattgttga cgagtaattt gccacggcaa atcgagcaac ggatcgccgt tatcccgtgc
    23581 cagcacgatc aattgcccgc tattttcctg ccagtccagc tcggcatcca ccagtgaagg
    23641 ctcctgcggc aagtttaacg tcgcagtagc atgaccactt acaggaagtc catccggcac
    23701 gagcggcata gtaaattccc ccaccagttt aaccggcgac tgattttcaa acgcgacgac
    23761 atccagttcg ctgactgtaa gttgttgccc tttcagctgg ccttgaaatt taactttttc
    23821 gccctgataa cgcagttgct ggatatcaga ggttaatgcg agagagagtt ttccctgcca
    23881 ttcctgccag ggagaaaaaa tcagtttatc gatattgatc caggtgttag gcagcatgga
    23941 ctgccactgc gcgagggttt ttggagcagc tggcgattgc tccgtctgcg gcaatttcgc
    24001 cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc tgggatgtga
    24061 aaggctggcg ttggtgatat gcgcaagctg acaatctccc accagataac ggagatcggg
    24121 aatgattaaa cctttacgcg taatgcgtgg gctttcatct aatgcaatac gtgtcccgag
    24181 cggtagccag atgcccgcca gcgtgggaac ccacagcccg agcgtcatca gcagcgtcaa
    24241 cggcacaaga ataatcagta ataacagcgc gagaacggct ttatatttac ccagcatggg
    24301 tagttaatat cctgatttag cgaaaaatta agcattcaat acgggtattg tggcatgttt
    24361 aaccgttcag ttgaaggttg cgcctacact aagcatagtt gttgatgaat ttttcaatat
    24421 cgccatagct ttcaattaaa tttgaaattt tgtaaaatat ttttagtagc ttaaatgtga
    24481 ttcaacatca ctggagaaag tcttatgaaa ctcgccgttt atagcacaaa acagtacgac
    24541 aagaagtacc tgcaacaggt gaacgagtcc tttggctttg agctggaatt ttttgacttt
    24601 ctgctgacgg aaaaaaccgc taaaactgcc aatggctgcg aagcggtatg tattttcgta
    24661 aacgatgacg gcagccgccc ggtgctggaa gagttgaaaa agcacggcgt taaatatatc
    24721 gccctgcgct gtgccggttt caataacgtc gaccttgacg cggcaaaaga actggggctg
    24781 aaagtagtcc gtgttccagc ctatgatcca gaagccgttg ctgaacacgc catcggtatg
    24841 atgatgacgc tgaaccgccg tattcaccgc gcgtatcagc gtacccgtga cgctaacttc
    24901 tctctggaag gtctgaccgg ctttactatg tatggcaaaa cggcaggcgt tatcggtacc
    24961 ggtaaaatcg gtgtggcgat gctgcgcatt ctgaaaggtt ttggtatgcg tctgctggcg
    25021 ttcgatccgt atccaagtgc agcggcgctg gaactcggtg tggagtatgt tgatctgcca
    25081 accctgttct ctgaatcaga cgttatctct ctgcactgcc cgctgacacc ggaaaactac
    25141 catcttttga acgaagccgc cttcgatcag atgaaaaatg gcgtgatgat cgtcaatacc
    25201 agtcgcggtg cattgattga ttctcaggcg gcaattgaag cgttgaaaaa tcagaaaatt
    25261 ggttcgttgg gtatggacgt gtatgagaac gaacgcgatc tgttctttga agataaatcc
    25321 aacgacgtga tccaggatga cgtattccgt cgcctgtctg cctgccacaa cgtgctgttt
    25381 accgggcacc aggcattcct gacagcagaa gctctgacca gtatttctca gactacgctg
    25441 caaaacttaa gcaatctgga aaaaggcgaa acctgcccga acgaactggt ttaatcttgc
    25501 cgctcccctg cattccaggg gagcttattc agataatccc caaagacctt tcatcttcta
    25561 ttcttaaaat agccctgagt cagaaactgt aattgagaac cacaatgaag aaagtagccg
    25621 cgtttgttgc gctaagcctg ctgatggcgg gatgtgtaag taatgacaaa attgctgtta
    25681 cgccagaaca gctacagcat catcgctttg tgctggaaag cgtaaacggt aagcccgtga
    25741 ccagcgataa aaatccgcca gaaatcagct ttggtgaaaa aatgatgatt tccggcagca
    25801 tgtgtaaccg ctttagcggt gaaggcaaac tgtctaatgg tgaactgaca gccaaagggc
    25861 tggcaatgac ccgtatgatg tgcgctaacc cgcagcttaa tgaactcgat aacaccatta
    25921 gcgaaatgct gaaagaaggt gcacaagtgg atctgaccgc gaaccagtta acgctggcga
    25981 ccgcgaaaca gacattaact tataagctgg cggatttaat gaattaatag ctgccacagc
    26041 tcccggcggc aagtgactgt tcgctacagc gtttgccgtt gggtaatgca cacatcccaa
    26101 tcgccgtacc atccagttga cgggcaacag aaagcgaacc gccgatcatt gcacaatttg
    26161 cttctccact actggacatc gacgctttta aacctggcgc tacgtgcgcc gcagtcgcct
    26221 gctgaacagg ttcactacta cacgccgaca acaataaagc ggcacaccct acccaaaacg
    26281 ctgctcgcat ctcttcttcc tctgatcttc aagccaaacg acaccgccat aaataatagg
    26341 cagcacagag ggcggcgtcg agagctgtcc tgcgcgttgc cccgcatttt tactttttta
    26401 tggctatttt tttgccctct gtttgatcaa aacattcatt acgctgatgt gggggacaca
    26461 aaagcgaaaa tgcagaagaa agccatttgc taaaattgaa ggattactac tgggcgcgca
    26521 gcaatttcgt gcgcccctca tttgcgcaat gtaagggtgt catatgatta ctattgacgg
    26581 taatggcgcg gttgcttcgg tggcgtttcg caccagtgaa gttatcgcca tctaccctat
    26641 tacccccagt tccacgatgg cagaacaggc tgatgcctgg gccggaaacg gcttgaagaa
    26701 cgtttgggga gacacaccac gcgtggttga aatgcagtcg gaagcgggtg ctatcgctac
    26761 cgtgcatggc gctttgcaga cgggtgccct ttcaacatcg tttacgtcat cgcagggttt
    26821 gctgctgatg atcccgacgc tgtacaaact ggcaggcgaa ctcacaccgt ttgtcctgca
    26881 tgtagcggca cgtaccgttg ccacacatgc actctctatt tttggcgatc attccgacgt
    26941 catggcggtg cgccagacgg gttgcgcgat gttgtgtgcg gcaaacgtcc aggaagcgca
    27001 agactttgct ctcatttcgc acatcgcgac gctgaaaagc cgcgtgccat ttattcattt
    27061 ctttgatggt ttccgcacgt cccacgaaat caacaaaatt gtcccgctgg ccgatgacac
    27121 gattcttgat ctcatgccgc aggccgaaat tgatgctcat cgcgcccggg cactcaaccc
    27181 ggaacatccg gtgatccgcg gtacgtccgc caatcctgac acttatttcc agtctcgcga
    27241 agccaccaac ccatggtacg acgcggtcta tgaccatgtt gaacaggcga tgaatgattt
    27301 ctctgccgcg acaggtcgtc agtatcagcc gtttgaatat tacgggcatc cgcaagcgga
    27361 acgggtgatt atcctgatgg gctctgccat tggcacctgt gaagaagtgg ttgatgaatt
    27421 gctaatccgt ggcgaaaaag tcggcgtgct gaaagttcgc ctgtaccgcc ccttctccgc
    27481 caaacacttg ctgcaagctc tgccgggatc cgtacgcagc gtggcggtac tggacagaac
    27541 caaagaaccc ggtgcccagg cagaaccgct ctatctggat gtgatgaccg cactggcaga
    27601 agcctttaat aatggtgagc gcgaaaccct gccccgtgtc attggtgggc gctatggtct
    27661 ttcatccaaa gaatttggcc cggactgtgt actggcggta tttgccgagc tcaacgcggc
    27721 taaaccgaaa gcgcgcttta cggttggtat ttacgatgat gtgactaatc tgtcactgcc
    27781 gttgccggaa aacaccctgc caaactcggc gaaactggaa gccttgtttt atggccttgg
    27841 tagtgatggc agcgtttccg cgaccaaaaa caatatcaag attatcggta attccacgcc
    27901 gtggtacgca cagggctatt ttgtttacga ctccaaaaaa gcgggcggcc tgacggtttc
    27961 tcaccttcgc gtgagcgaac agccgattcg ttccgcttat ctcatttccc aggctgattt
    28021 tgttggctgc caccagttgc agtttatcga taaatatcag atggctgagc gtttaaaacc
    28081 tggcggcatt ttcctgctca acacaccgta cagcgcagat gaagtgtggt cgcgcttgcc
    28141 gcaagaagtt caggccgtgt taaaccagaa aaaagcgcgc ttctatgtga ttaacgcggc
    28201 gaaaatcgcc cgcgaatgtg gcctggcggc ccgtattaat accgtcatgc agatggcttt
    28261 tttccatctg acgcaaattc tgcctggcga tagcgccctc gcagaattgc agggtgcgat
    28321 tgccaaaagt tacagtagca aaggccagga tctggtggaa cgcaattggc aggctctggc
    28381 gctggcgcgt gaatccgtag aagaagttcc gttgcaaccg gtaaatccgc acagcgccaa
    28441 tcgaccgcca gtggtttccg atgccgctcc tgatttcgtg aaaaccgtaa ccgctgcgat
    28501 gctcgccggg cttggtgacg ccctccccgt ttcggcgctg ccgccagacg gctcctggcc
    28561 gatgggcact acgcgctggg aaaaacgtaa tattgccgaa gagatcccca tctggaaaga
    28621 ggaactctgt acccaatgta accactgcgt tgccgcttgc ccacactcag ctattcgcgc
    28681 aaaagtggtg ccgcctgaag cgatggaaaa cgcccctgcc agcctgcatt cgctggatgt
    28741 gaaatcgcgt gatatgcgcg ggcagaaata tgtcttgcag gtggctccgg aagattgcac
    28801 cggttgtaac ctgtgcgtcg aagtttgccc ggcgaaagac cgtcagaatc cagagattaa
    28861 agccatcaat atgatgtcgc gcctggaaca tgtcgaagaa gagaaaatca attacgattt
    28921 cttcctcaac ctgccagaaa tcgaccgtag caaactggaa cgtattgata ttcgtacatc
    28981 gcagctgatt acaccgctgt ttgaatattc aggtgcttgc tccggttgtg gcgagacgcc
    29041 gtatattaaa ttactgactc agctctatgg cgaccggatg ttgatcgcta acgccaccgg
    29101 ctgttcttca atttatggcg gtaacctgcc ctctacaccg tataccaccg atgccaacgg
    29161 tcgtgggccg gcatgggcga actctctatt tgaagataat gccgaatttg gccttggttt
    29221 ccgcctgacg gtcgatcaac accgtgtccg cgtgctgcgt ctgctggatc aatttgccga
    29281 taaaatcccg gcggaattac tgacggcgtt gaaatcagac gctacgccag aggttcgtcg
    29341 agaacaggtc gcagctttac gccagcaact caacgatgtt gccgaagcac atgaactgct
    29401 acgtgatgca gatgcactgg tggaaaaatc aatctggctg attggtggtg atggctgggc
    29461 ttacgatatc ggctttggcg gtctggatca tgtattgagt ttgacggaaa acgtcaacat
    29521 tctggtgctg gatacgcaat gctattccaa caccggtggt caggcatcga aagcgacacc
    29581 gctgggtgca gtaactaaat ttggcgagca cggcaaacgt aaagcgcgta aagatcttgg
    29641 cgtcagtatg atgatgtacg gtcatgttta tgtggcgcag atttctctcg gcgcgcagct
    29701 gaaccagacg gtgaaagcga ttcaggaagc ggaagcgtat ccggggccat cgctgatcat
    29761 tgcttatagc ccgtgtgaag agcatggtta cgatctggca ctcagccacg accagatgcg
    29821 ccaactcaca gctaccggct tctggccgct atatcgcttc gatccgcgtc gtgccgatga
    29881 aggcaaactg ccgctggcgc tggattcacg cccgccgtca gtagcactgg aagaaacgtt
    29941 acttcacgag caacgtttcc gtcggctgaa ttcgcagcag ccagaagtgg cagaacagtt
    30001 atggaaagat gctgcagctg atttgcaaaa acgctatgac ttcctggcac aaatggccgg
    30061 aaaagcggaa aaaagcaaca ccgattaaat gctctggata aggattatcc aattctaaaa
    30121 aaaaagcccg gacgactgtt cgggcttgtc tttttatatg ttggaaaatc agtggcaatg
    30181 caatggccca acagaaaata aaatctgtga caataaagac atataacccg cgcagaataa
    30241 cgtatagaaa attaaaagta taatttttat tttgtatgta ttatcttcga t
//
LOCUS       DBANUU010000053        29663 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000053 DBANUU010000000
VERSION     DBANUU010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.053"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            87..1709
                     /gene="yhiJ"
                     /locus_tag="ACIE68_002238"
     CDS             87..1709
                     /gene="yhiJ"
                     /locus_tag="ACIE68_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFX5908549.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSDMRAHILRR
                     DNNYQFIVTINSKNYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPVWDWK
                     ISQFNVSDIKFETMINPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLRIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYAGIKDYPVCFYDPKYKIMANHHGITFDEQRKRYIIGPIT
                     VSIDEMTNALDPVELAAIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            1899..2012
                     /gene="yhiY"
                     /locus_tag="ACIE68_002239"
     CDS             1899..2012
                     /gene="yhiY"
                     /locus_tag="ACIE68_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFX5908550.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            2075..3142
                     /gene="yhiI"
                     /locus_tag="ACIE68_002240"
     CDS             2075..3142
                     /gene="yhiI"
                     /locus_tag="ACIE68_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFX5908551.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAHRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            3139..5874
                     /gene="rbbA"
                     /locus_tag="ACIE68_002241"
     CDS             3139..5874
                     /gene="rbbA"
                     /locus_tag="ACIE68_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFX5908552.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5874..6998
                     /gene="yhhJ"
                     /locus_tag="ACIE68_002242"
     CDS             5874..6998
                     /gene="yhhJ"
                     /locus_tag="ACIE68_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFX5908553.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            7102..7332
                     /locus_tag="ACIE68_002243"
     CDS             7102..7332
                     /locus_tag="ACIE68_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFX5908554.1"
                     /translation="MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMS
                     VACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            7329..7688
                     /locus_tag="ACIE68_002244"
     CDS             7329..7688
                     /locus_tag="ACIE68_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFX5908555.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(7808..8209)
                     /gene="nikR"
                     /locus_tag="ACIE68_002245"
     CDS             complement(7808..8209)
                     /gene="nikR"
                     /locus_tag="ACIE68_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFX5908556.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(8215..9021)
                     /gene="nikE"
                     /locus_tag="ACIE68_002246"
     CDS             complement(8215..9021)
                     /gene="nikE"
                     /locus_tag="ACIE68_002246"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFX5908557.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(9018..9782)
                     /gene="nikD"
                     /locus_tag="ACIE68_002247"
     CDS             complement(9018..9782)
                     /gene="nikD"
                     /locus_tag="ACIE68_002247"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFX5908558.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(9782..10615)
                     /gene="nikC"
                     /locus_tag="ACIE68_002248"
     CDS             complement(9782..10615)
                     /gene="nikC"
                     /locus_tag="ACIE68_002248"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFX5908559.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(10612..11556)
                     /gene="nikB"
                     /locus_tag="ACIE68_002249"
     CDS             complement(10612..11556)
                     /gene="nikB"
                     /locus_tag="ACIE68_002249"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFX5908560.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(11556..13130)
                     /gene="nikA"
                     /locus_tag="ACIE68_002250"
     CDS             complement(11556..13130)
                     /gene="nikA"
                     /locus_tag="ACIE68_002250"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFX5908561.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(13241..13828)
                     /gene="acpT"
                     /locus_tag="ACIE68_002251"
     CDS             complement(13241..13828)
                     /gene="acpT"
                     /locus_tag="ACIE68_002251"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFX5908562.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(13883..14932)
                     /gene="yhhT"
                     /locus_tag="ACIE68_002252"
     CDS             complement(13883..14932)
                     /gene="yhhT"
                     /locus_tag="ACIE68_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFX5908563.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            15064..16281
                     /gene="yhhS"
                     /locus_tag="ACIE68_002253"
     CDS             15064..16281
                     /gene="yhhS"
                     /locus_tag="ACIE68_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5908564.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     SVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(16285..16842)
                     /gene="dcrB"
                     /locus_tag="ACIE68_002254"
     CDS             complement(16285..16842)
                     /gene="dcrB"
                     /locus_tag="ACIE68_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFX5908565.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(16915..17580)
                     /gene="yhhQ"
                     /locus_tag="ACIE68_002255"
     CDS             complement(16915..17580)
                     /gene="yhhQ"
                     /locus_tag="ACIE68_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFX5908566.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            17801..18046
                     /gene="tusA"
                     /locus_tag="ACIE68_002256"
     CDS             17801..18046
                     /gene="tusA"
                     /locus_tag="ACIE68_002256"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFX5908567.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(18148..20346)
                     /gene="zntA"
                     /locus_tag="ACIE68_002257"
     CDS             complement(18148..20346)
                     /gene="zntA"
                     /locus_tag="ACIE68_002257"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFX5908568.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGNVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(20420..21046)
                     /gene="yhhN"
                     /locus_tag="ACIE68_002258"
     CDS             complement(20420..21046)
                     /gene="yhhN"
                     /locus_tag="ACIE68_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFX5908569.1"
                     /translation="MLWSFIAVCLSTWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            21187..21546
                     /gene="yhhM"
                     /locus_tag="ACIE68_002259"
     CDS             21187..21546
                     /gene="yhhM"
                     /locus_tag="ACIE68_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFX5908570.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(21549..21818)
                     /gene="yhhL"
                     /locus_tag="ACIE68_002260"
     CDS             complement(21549..21818)
                     /gene="yhhL"
                     /locus_tag="ACIE68_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFX5908571.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(21808..22404)
                     /gene="rsmD"
                     /locus_tag="ACIE68_002261"
     CDS             complement(21808..22404)
                     /gene="rsmD"
                     /locus_tag="ACIE68_002261"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFX5908572.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            22554..24047
                     /gene="ftsY"
                     /locus_tag="ACIE68_002262"
     CDS             22554..24047
                     /gene="ftsY"
                     /locus_tag="ACIE68_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFX5908573.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            24050..24718
                     /gene="ftsE"
                     /locus_tag="ACIE68_002263"
     CDS             24050..24718
                     /gene="ftsE"
                     /locus_tag="ACIE68_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFX5908574.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            24711..25769
                     /gene="ftsX"
                     /locus_tag="ACIE68_002264"
     CDS             24711..25769
                     /gene="ftsX"
                     /locus_tag="ACIE68_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFX5908575.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            26014..26868
                     /gene="rpoH"
                     /locus_tag="ACIE68_002265"
     CDS             26014..26868
                     /gene="rpoH"
                     /locus_tag="ACIE68_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFX5908576.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            27140..28243
                     /gene="livJ"
                     /locus_tag="ACIE68_002266"
     CDS             27140..28243
                     /gene="livJ"
                     /locus_tag="ACIE68_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFX5908577.1"
                     /translation="MNMKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            28393..28659
                     /gene="ataR"
                     /locus_tag="ACIE68_002267"
     CDS             28393..28659
                     /gene="ataR"
                     /locus_tag="ACIE68_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin AtaR"
                     /protein_id="HFX5908578.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            28666..29193
                     /gene="ataT"
                     /locus_tag="ACIE68_002268"
     CDS             28666..29193
                     /gene="ataT"
                     /locus_tag="ACIE68_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     acetyltransferase AtaT"
                     /protein_id="HFX5908579.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(29190..29573)
                     /gene="panM"
                     /locus_tag="ACIE68_002269"
     CDS             complement(29190..29573)
                     /gene="panM"
                     /locus_tag="ACIE68_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFX5908580.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLCVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         7142 a         7579 c         8154 g         6788 t
ORIGIN      
        1 attatttttc catcgaacat gaggtgtatg tcataagatt aaatgttaac tatgcattcc
       61 tcgcggtgaa ttatatgagg taaaagatga aaattggaac tgtggcaggc actaacgaca
      121 gtacaacgac aatcgcaaca aatgatatgg tgcaagagca tgttactaac ttcactaaag
      181 aactgtttgg gtatattgca aatggtatag gtgatgatat atcaagtatt gccaggacaa
      241 tgctcggtga agttgtggaa aaaattgatg attggcaaat agaacgtttc caacaatcta
      301 ttcgagatga caaaatatca tttaccatac aaacgaatca ttcagaaaaa tatagtatgc
      361 tatcggacat gcgcgctcat attctacgta gggataataa ttatcagttt attgtgacaa
      421 ttaatagtaa aaattacggc tgctatcttg ataataccga tattaattgg tgcagcattg
      481 tttatttact taataatatg acagtaaatg atgatgctaa tgatgttgca gttactgagt
      541 cgtacaaacc cgtctgggac tggaaaatat cgcaattcaa tgtatctgat attaaattcg
      601 aaacaatgat aaacccccag ttcgctgaca gaacctattt ctccaattgt tcacctgttg
      661 atcccacaag taccagacca acctattttg gcgataccga tggctcagtc ggtgcagttt
      721 tgtacgcgct ttttgcaacg ggacatttga gaatcatggc tgaaggagaa aatttcctta
      781 gccagttgct taatatagaa gatgaagttc tcaacgtatt gctaagggag aactttaatg
      841 agcaacttga cactaatgtt aatacaataa tcagtattct gaacagaaga gatatagttt
      901 tagaaagtct tcagccttat ttagttatta ataaagacgc tgtgacgcca tgtacgtttc
      961 ttggcgacca aaccggcgat cgatttagta atatctgtgg cgatcaattt atcatcgatt
     1021 tgttaaaacg cataatgagt atcaatgaaa atgttcatgt attagctgga aatcatgaaa
     1081 cgaattgcaa tggtaactat atgcagaact tcacgcgtat gaaaccactt gatgaggata
     1141 cctacgctgg gataaaagat tatcctgtat gtttctatga ccccaaatac aaaataatgg
     1201 caaatcatca tgggataact tttgatgagc agcgaaaacg ttacattata ggaccgataa
     1261 cagtctctat tgatgaaatg accaatgctc tcgatccggt cgaattagcg gcaattatca
     1321 ataaaaagca tcatgctata attaatggca aaaagtttaa aactagccgg gctatctctt
     1381 gtcgatcctt taatcgttat ttttctgttt cgacagatta cagacctaaa cttgaagcac
     1441 tattagcatg ttcacaaatg ttaggtataa atcaagttgt tgcacataac ggaaatggtg
     1501 gaagagaacg cataggcgaa acggggacag ttttaggtct taacgcccgt gacagtaaac
     1561 atgctggaag aatgttcagc atgcataatt gccaaattaa ccctggtgca gggcctgaga
     1621 taaccactcc ctggaagtct taccagcatg aaaaaaacag aaatggactt atgcctttga
     1681 ttagaaggcg cacaatgtta cagctctgaa ccatagacaa catgaatatt aatgttacta
     1741 tctggttgag actgttgata aaacgtaaaa aggaatgctc tgttcctgca actgaacagg
     1801 cgtgaaacgc tatccaacag gatggatacc gttttgtcag ccagttttat agatcacact
     1861 tatgagtatg cgtgtttcat ttacggccag gaatgctgat gatgacaaca ttactacccg
     1921 ttttcactaa gccttccccg ttggcgctca atgctctgcg cgctggtcgt atttgccgtt
     1981 tccttcttat cccggatgga agaatccgct gaacgtcgtt cggcggcagt tgtaacacgc
     2041 gtcgtttgct tacagggatt atggcgggtc gattatggat aagagtaagc gccatctggc
     2101 gtggtgggtt gtcgggttac tggcggtggc ggctatcgtg gcgtggtggc tgttgcgccc
     2161 ggcaggtgtg ccggaaggct ttgctgtcag caatgggcgc attgaagcga cggaagtgga
     2221 tatcgccagc aaaattgccg ggcgtattga caccattctg gtgaaagaag gccagtttgt
     2281 tcgagaaggt gaagtgctgg cgaagatgga tactcgcgtg ttgcaggaac agcgactgga
     2341 agccatcgcg caaatcaaag aagcacaaag cgccgttgct gccgcgcagg ctttgctgga
     2401 gcaacgccaa agtgaaactc gcgccgctca gtcgctggtt aatcaacggc aagctgaact
     2461 ggactccgta gccaaacgtc atacccgttc ccgctcgctc gcccatcgtg gggctatttc
     2521 tgcgcaacag ttggatgacg accgcgccgc cgctgagagc gcccgagctg cgctggaatc
     2581 ggcaaaagct caggtttcgg cttctaaagc cgctatagaa gcggcacgca ccaatatcat
     2641 tcaggcgcaa actcgcgtcg aagccgcaca agccactgaa cggcgcattg ccgcagatat
     2701 cgatgacagc gaactgaaag ccccgcgtga cggacgcgtg cagtatcggg ttgccgagcc
     2761 aggcgaagtg ctggcggcag gcggtcgggt gctgaatatg gtcgatctca gcgacgtcta
     2821 tatgactttc ttcctgccaa ccgaacaggc gggcacgctg aaactgggcg gtgaagcccg
     2881 gctgatcctc gatgccgcgc cagatctgcg tattcctgca accatcagtt ttgtcgccag
     2941 tgtcgcccag ttcacgccaa aaaccgtcga aaccagcgat gaacggctga aactgatgtt
     3001 ccgcgtcaaa gcgcgtatcc caccggaatt actccagcag catctggaat atgtcaaaac
     3061 cggtttaccg ggcgtagcgt gggtgcgggt gaatgaagaa cttccgtggc ctgacgacct
     3121 cgtggtgagg ttgccgcaat gacgcatctg gaactggttc ccgtcccgcc tgtcgcgcaa
     3181 ctggcgggcg tgagccagca ttatggaaaa accgttgcgc tgaacaatat cacactcgat
     3241 attccggccc gttgcatggt cgggctgatt ggcccggacg gcgtcgggaa gtcgagcttg
     3301 ttgtcgttga tttccggtgc ccgcgtcatt gaacagggca atgtgatggt gctgggcggc
     3361 gatatgcgcg acccgaagca tcgccgcgac gtctgcccgc gcatcgcctg gatgccgcag
     3421 gggctgggca aaaatctcta ccacacctta tcggtgtatg aaaatgtcga ttttttcgct
     3481 cgcttgttcg gtcacgataa agcggagcgg gaagtccgaa ttaatgagct gctgaccagc
     3541 accgggttag caccgtttcg cgatcgtccg gcaggtaaac tctccggcgg gatgaagcaa
     3601 aaactggggc tgtgctgcgc gttaatccac gacccggaac tgttgatcct tgatgagcca
     3661 acaacggggg ttgacccgct ctcccgcgcc cagttctggg atctgatcga cagtattcgc
     3721 cagcggcaga gcaatatgag cgtgctggtc gccaccgcct atatggaaga ggccgaacgc
     3781 ttcgactggc tggtagcgat gaatgccgga gaagtgctgg caactggcag cgccgaagag
     3841 ctgcggcagc aaacgcaaag cgccacgctg gaagaggcgt ttataaatct gttaccgcaa
     3901 gcgcaacgcc aggcgcatca ggcggtagtg atcccaccgt atcaacctga aaatgcagag
     3961 attgccatcg aagcgcgcga tctgaccatg cgttttggtt ccttcgttgc cgttgatcac
     4021 gtcaatttcc gcattccacg cggggagatt tttggttttc ttggatcgaa cggctgcggt
     4081 aaatccacca ccatgaaaat gctcaccgga ctgctgcccg ccagcgaagg tgaggcgtgg
     4141 ctgtttgggc aaccggttga tccaaaagat atcgataccc gccgtcgggt gggctatatg
     4201 tcgcaggcgt tttcgctcta taacgaactc accgtgcggc aaaaccttga gttacatgcc
     4261 cgtttgtttc acatcccgga agcggaaatt cccgcgcgag tggctgaaat gagcgagcgt
     4321 tttaagctca acgacgttga agatgttctg ccggagtcat tgccgctcgg cattcgccag
     4381 cggctttcgc tggcggtggc ggtgattcat cgcccggaga tgttaatcct cgatgaacct
     4441 acttctggcg tcgatccggt ggcgagggat atgttctggc agttgatggt cgatctctcg
     4501 cgccaggaca aagtaaccat ttttatctcc acccacttta tgaacgaagc ggaacgttgc
     4561 gaccgcatct cactgatgca cgccggaaaa gtgctcgcca gcggtacacc gcaggaactg
     4621 gttgagaaac gcggagccgc cagtctggaa gaggcattta tcgcctattt gcaggaagcg
     4681 gcaggacaga gcaacgaagc cgaagcgccg cccgtgatac acgacaccac ccacgcgccg
     4741 cgtcagggat ttagcctgcg ccgtctgttt agctacagcc gccgcgaagc actggaactg
     4801 cgacgcgatc cggtacgttc gacgctggcg ctgatgggaa cagtgatcct gatgctgata
     4861 atgggttacg gcatcagtat ggatgtggaa aacctgcgct ttgcggtgct cgaccgcgac
     4921 cagaccgtca gtagccaggc gtggacgctc aatctctccg gttcccgtta ctttatcgaa
     4981 cagccgccgc tcaccagtta tgacgagctt gatcgtcgga tgcgtgcggg cgatataacg
     5041 gtggcaattg agatccctcc caatttcggg cgcgatatcg cgcgtggtac gcctgtggaa
     5101 ctcggcgtct ggatcgacgg agcgatgccg agccgtgccg aaacggtaaa aggttacgtg
     5161 caggcaatgc accagagctg gctacaggat gtggcgagcc gacaatccac acccgccagc
     5221 caaagcgggc tgatgaatat tgagacgcgc tatcgctata acccggacgt aaaaagcctg
     5281 ccagcgattg ttccggcggt gatcccgctt ctgctgatga tgatcccgtc aatgctaagc
     5341 gcccttagcg tggtgcggga aaaagagctt gggtcgatta tcaaccttta cgtgaccccc
     5401 accacgcgta gtgaattttt gcttggtaaa cagttgccat acatcgcgct ggggatgctg
     5461 aactttttcc tgctctgcgg cctgtcggtg tttgtgtttg gcgtaccgca taaaggcagt
     5521 ttcctgacgc tcaccctggc ggcgctgctg tatatcatca ttgccaccgg aatggggctg
     5581 ctgatctcca cctttatgaa aagccagatt gccgccattt tcggaacggc gattatcacg
     5641 ttgatcccgg cgacacagtt ttccgggatg atcgatccgg tagcttcgct ggaagggcct
     5701 ggacgttgga tcggcgaggt ttacccgacc agtcattttc tgactatcgc ccgcggaacg
     5761 ttctcgaaag cgctggatct gactgatttg tggcaacttt ttatcccgtt gctgatagcc
     5821 atcccgctgg tgatgggctt aagtatcctg ctgctgaaaa aacaggaggg atgatgcgcc
     5881 atttacgcaa tatttttaat ctgggtatca aagagttgcg cagtctgctc ggtgataaag
     5941 cgatgctgac gctgattgtc ttctcgttta cggtgtcggt gtattcgtca gcgaccgtta
     6001 cgccaggatc gttgaacctc gcgccgatcg ccattgccga tatggatcaa tcgcagttat
     6061 cgaaccggat cgttaacagc ttctatcgcc cgtggttttt gccaccggag atgatcaccg
     6121 ccgatgagat ggatgccgga ctggacgccg ggcgttatac ctttgcgata aatattccgc
     6181 ctaattttca gcgtgatgtc ctcgccggac gccagccgga tattcaggtg aacgtcgatg
     6241 ccacgcgcat gagccaggca tttaccggca atgggtatat ccagaatatt atcaacggtg
     6301 aagtgaacag ctttgtcgcg cgctaccgtg ataacagcga accgttggta tcgctggaaa
     6361 cccggatgcg ctttaacccg aaccttgatc ccgcgtggtt tggcggggtg atggcaatca
     6421 tcaacaacat taccatgctg gcgattgtat tgaccggatc ggcgctgatc cgcgagcgtg
     6481 aacacggcac ggtggaacac ttgttggtga tgccgataac gccgtttgag atcatgatgg
     6541 caaaggtctg gtcgatgggg ctggtggtgc tggtggtgtc gggattatca ctggtgctga
     6601 tggtgaaagg cgtgctgggt gtaccgattg aaggctcgat cccgctgttt atgctgggcg
     6661 tggcgctcag tctgtttgcc accacgtcaa tcggcatttt tatggggacg atagcgcgtt
     6721 caatgccgca actggggctg ctggtgattc tggtgctgct gccgctgcaa atgctttccg
     6781 gtggatccac gccgcgcgaa agtatgccgc agatggtgca ggacattatg ctgaccatgc
     6841 cgacgacaca ctttgttagc ctcgcgcagg ccattctcta ccggggtgcc ggattcgaaa
     6901 tcgtctggcc gcagttcctg acgctgatgg caattggcgg cgcgtttttc accatcgcgc
     6961 tgctgcgatt caggaagacg attgggacaa tggcgtaagg aagggaaact tgacttcata
     7021 ctgtgatgtc ataaagtgaa gtcataaggg aatgtcatgt aaacacaggt acaggcttta
     7081 cgaaagaaac aaaaaaacac actggatcag ctatttaaat cacctttacc tcaaggaatc
     7141 aaatggactg atatagaatc tctggttaaa gcgttaggcg gagaaattaa ggaaggtagg
     7201 gggtcgcgct gtaagttcat actaaatatg agcgttgcgt gtttccatcg gcctcatccg
     7261 tcgccagata ccgataaagg cgctgtagaa agcgtgcgtg actggttact aagtataggg
     7321 gtaaaaccat gatcaaactg aagacgccaa actctatgga gattgccggg caacctgctg
     7381 ttatcactta cgtgccagaa ctgaatgcat ttcgtggcaa attcctgggc ttgtcaggtt
     7441 attgtgattt tgtctctgac agcattcagg ggctgcaaaa agaaggggaa ctttccctgc
     7501 gagagtatct tgaggattgt aaagctgcgg gtattgagcc gtatgcgcgt acagaaaaga
     7561 ttaagacctt tacgttacgc tatccagaat cgctgagtga acgccttaat aatgcggctg
     7621 cgcagcaaca agtctctgtt aatacctata ttattgaaac actgaatgag cgtttgaatc
     7681 atttataaat tttagcggcg tacattacgc agaatatctg cctttacagg atctatctat
     7741 gcaactgaac ttaattttat aaattaagtt cagttaatat cttttctttc ctcgtttcag
     7801 aaataactca atcttccttc ggcaagcact gcaaatgccc gtgccgcacg ccgcgctggg
     7861 cgataacgtc atcggcaaaa tgctgcacgt cgcccatatc acctttcagc acggcgattt
     7921 ccagacagtc gtcgtgattg atatgcacat gcagcgtggc gacggagaga tcgtggtgat
     7981 ggtgctgggt ggagacaatg cggctggcta agtcgcgttt ttcgtgttca tacacatagg
     8041 agagcaccgc gaaaccttgc gtgccgtgct gctgagtggc ctcctgggcc agggcgctac
     8101 gcagaatatc gcggatagct tcggaacggt tgttataacc acgacgctgg ctcaggctgt
     8161 ccagcgtctc cagtaaatcg tcatcaagcg tgatggtgac tcgttgcatt tgcgttaaac
     8221 cttttctgtg gtgcgacggc gcacggggaa tgcgggtaat accgcgtttt gtagcacacg
     8281 tccggcgtca gaggaaaagg ttaatttatc tcccaccacc tgggtttcga cgatttgtcc
     8341 gttgtccatc accatcaccc gctgacagaa gcgttccacc aggcgtaagt cgtgggtgat
     8401 gaacaggcag gcggtgccaa actgttgttg tagctttttc agcaggcgaa tgacgcccgc
     8461 ctgtaacacg agatcaaggt tagaaacggc ttcatccaga atcagtagtt tcggttcgac
     8521 cgccagcgcg cgagccaggc agacgcgctg gagctggccg ccgcttaact gcggtgggcg
     8581 tttgtcgaga acgctgtcat cgagatcgac cgccttcagc atttcgctgg cgcgcgccag
     8641 ttgttcggat tttttcagtg atagcaggtg gcgcatcggt tcacgcagga tctcgcgcac
     8701 ggttttgcgc ggattcacgg cgctgatgga gtcctgaaat accatctgaa tatcgcggcg
     8761 gaacgcttta cgctgggcgc gattgagttt cgccagcggt tcgccgcgcc agctaatatt
     8821 cccttgtgag ggagactcta agcctaccag cagccgcgcg agggtgcttt ttccgcagcc
     8881 gctgcgtccc agcagggcga cggtttcgcc gcttttcagg gtcagggaaa cgttattcag
     8941 caccgcctga tgttgatgtt ttccgctaaa tccaccgtgg gcatagtgat gggaaaggcc
     9001 gcagacgtta agtaaggtca tgatgccagc tccataccgt agagggcgag atgagcggaa
     9061 accaggctgc gcgtcaccgt atgtttgggg gcgttaaaca gcgtttctac atcgccctgt
     9121 tcgacaattt taccttgtga catcaccgcc acgtcatccg ccagacgcgc cacaacgccc
     9181 atatcatggg tgaccagcag cattcccggc gcttgttttt gcataatgct ttccagcaga
     9241 tcgaggatgc gcgcctgtgc taccacgtcg aggtcggtgg tcggttcatc ggcgatgata
     9301 aacggtgatt cacacagcac cgccatcgca atcatcatgc gctgcaacat gccgccgctc
     9361 atctcgaacg ggtacagctt cagcacgcgc gcggcgtttt ccagccccac cgcttctatg
     9421 gcagcggtaa gcgtagcgtc atcggcgggt ttccctaacg ccaggcaggt ttcccgcgcg
     9481 tgggtgtgca tggtgtgcag tggattaaag gcgctgcgcg ggttctgcat gatggtggca
     9541 attttgatgc cgcgcagggc gcaaggcgaa accggtttgc catcggctaa aatttccccc
     9601 gccgtctggc gaacgccagc gggcaaaatg cccagcgtcg cggcgcaggt taatgatttc
     9661 ccgctgccgc taccgccgac taacgccagc acgcgcccgc gttgcagggt taacgataca
     9721 ccgtgtacca gcggctgcgc ggcctgtagc gcgatattac gtagttcaat ctgttgcggc
     9781 attagtgtgc gtgctccgtc accagatgag gatccagatg atcgcgcagt gcgtcaccca
     9841 ccaggttaaa ggccatcacg ctgataaaca gcgccagccc cggccagaac atttgcagcg
     9901 gctgggtcca gatatactgg cgcgcgtcgt taatcatcac gccccattcg gcggtcggcg
     9961 cggtcacacc gaggccaagg aaagacatcc ccgcgacgtg cagcatcata tggccgatat
    10021 ccagcgttgc cagcaccagc agcgaaggga tcaccgcgcc tgccagatga tcgacaaaca
    10081 cccgcacatg gcccgcaccg gaaagccgtg acgccagcac aaactcgcgt tggcgtagtg
    10141 aaatcaccag gctgcgcacc atgcgtgcat accacgccca gtgcgacagg gcgatggcga
    10201 taattacgtt ggtcagcccg gtgccgagca cgccaaccat aaagaacgac agaatcgagg
    10261 tcgggaaggt cataaacata tcggcgacgc gcatggtggc ctgatcaacg cgtccgccaa
    10321 tcagtccggc gctgccgcca atcaccagcc ctaatgtcag caccagcagc aggcaggcca
    10381 ttaccgaacc gagcgacacg cgggtcgccg ccatcagccg cgagaaaata tcacgcccta
    10441 agtgatcggt gcccagccag tgctgcgcat ccggcgaaag caggcgcgac ggcagatcaa
    10501 tagcctgagg gtcatacggc agccaccact ggctggtaag cgcaatcagc gccagcaggg
    10561 cgataatgat cagcgccagg cgtaccgacc agcgggaaga gaggaaaaag ttcacgcgtg
    10621 cgctccttca tgacgacgaa tgcgcgggtc cagcgcggcg ttgagcaaat cgacaatcaa
    10681 attacagacc acaaaaacca ccaccatcat cagcgtaaag cactggatca ccggatagtc
    10741 acggttaaaa atcgccgaca ccgcatagcg cccgacgccc ggccaggcaa agatgttttc
    10801 gataatcatc gtcccgccga tcagttcgcc gatgtgcatc cccacggcgg tgatcatcgg
    10861 cagcgaggca ttgcgcagga tgtggcgacg ttcggtctgt ttgtcgttca ggccgcgcag
    10921 acgcgcccag gtgacgtgac gctgaccggc gacgtccagc atactggcgc gcagtaaacg
    10981 cgcgttaatc gccagcgaca taaaggcaat ggaaaccgca ggcaaaatga tgtgctgcca
    11041 gccgccgtaa cccatcgcgg gtagccattg cagatacacc gaaaacgcca tcaccagtaa
    11101 aaacgccagc cagaagttag gcatcgacac gccaagaaac gcgatgaaac gtacggcgaa
    11161 atccggcaga cggtcgcggt ggcgcgccgc ccagataccg agcggtacgg aagtgagcag
    11221 aattaatacc agcgccgcac ctgcaagttc cagcgtggcg ggcaggaagt tcagcatatc
    11281 gtccagtacc gggcgttggc tggcgaatga gataccaaag tcaagatgca gcgccttcca
    11341 caaccaggtg ccgtactgga cgtacagcgg ctgatccagt cccagcatgg tgcgggtaga
    11401 ggccagcatc tccggcgtcg gcggcaggtt agacagacgc aaataatcga gcgccgggtc
    11461 gccggtaccg aggcgcagca tcagaaaaat gatcaccgag gcggcgagca ccatcgggat
    11521 cagcagcaga aagcggcgta atacgtaacg caacattaag gtttcaccgg tttaatctgt
    11581 tcgaacggaa tttcggtggc gatcggcgcg taggggatgt tacccagctc cggttttgat
    11641 accaccatca tcgagatgta gctgataggc agataaaccg cctcgtcatg cagacgggtc
    11701 agaatgtcgc gatacagcgc ctgacgttgc gtttcgtcat gggtcgccag cacttcgccg
    11761 atctctttat caatcagcgg cttgtcggct aatccttgct gtgcctggaa gtcagcgtgc
    11821 gacggtacgc gcattgaact caggaaggcg tgtggatcgt atggcgcgcc ccaggtgcgg
    11881 tggaaaatca tgccaaaacg accgtcgcgc tgacgagcat agatactgct ctcttcttcg
    11941 ccaatcagcg agacatctgc gccaatctgg cgcatatcag cctgaatgat ttccgccatc
    12001 gatttgctta acgcatcggt gccgatgaac gacagttcaa tgcgcagcgg ctgaccattt
    12061 ttctcgcgga tgtctttgcc cgcaggcagc gtccaaccgg ctttttccag caactctttc
    12121 gctttctgcg gatcgtactg gcgcggtttc aggccgagat tagcgtaagg cacagaaggg
    12181 gcaaacaggg tgtcggcgac ctgctgggtg ccatataacg cgttatcaat cagcgatttt
    12241 ttgtttaccg cgtaattaag agcttcacgt actgccagct cgttggtggg ggctttggcg
    12301 gtattgagcg ccagcatcac ggtttcgatc ggctgtgaca gttgggtgtg gtaagccgga
    12361 ttctggctaa agcgggcgaa ggtatcgagc ggtaataacc cttcgtttcc gtacagcagg
    12421 tcgatatcgc cagtttcaaa cgccaccgcg cgggtagtcg ggtccgggat gacgttaaag
    12481 gtgatctttt taatcgctgg cttttcgccc cagtagtttt cgttacggac gaagacatcg
    12541 tactgattca gtttcgattc ctgcaaaatc cacggtccgg tgccaatcgg cgctttaatt
    12601 ccgttcatgg tttcatggtt tttaaactgc gagggagcga taaaacggaa aggacggggc
    12661 agggccagtt cttgcaggaa aggatagtag gcgcttttca gggtaatttg cagctctgtt
    12721 ttactgagtg ctttaacatc aacaatctgg tttgccagct ccagccaggc gtgacgttga
    12781 cggttatcga gcactgcgcg gaagttttct gccgccgcct cggcatcgaa cggttcaccg
    12841 ttggagaatt tcacgtcatc acgcagggtg aaggtccagg ttttaccatc ttctgaatga
    12901 gtccagcttt ttgccagcca cgggatcacc gaaccgtctg cctgatattt caccaatggt
    12961 tcataaacca tgctctgggc gaacatctgg ttaggcgtgt aaaggtgcgg gtttagtggc
    13021 ccgacattca ccggccaggc ggtggtgatt tcatctggtg cagcggcatg gacgataaaa
    13081 gacgcacaag ccagcagcgc aaatagagtg cggcggagtg tggagagcat ggtaacccca
    13141 atggattaaa atagatggcg gaaataagta tgacgatttt aagtattcgt catactgatt
    13201 acctgttctg gatcaataga tgggcagtcg ggtgggcgga tcagttaact gaatcgatcc
    13261 attgcacact gtcggcggtg agcgtaaagg gggtgggggt gcagatcgcc aggcttaaat
    13321 tttcaagctg acaatggctg actgacagcg aggagtgata ggtgctgtct acgctgacga
    13381 tttgccaggc gctgccgcca cgctgtttaa cgatggcttc tttgcgcgtc cagatgcgcc
    13441 agaacatttc cagttgctga tcaggatgca cggcgtccat ctcagcgtgt tccccgaggc
    13501 tgaatacggc gttcgccagc cagcgccagt tggcgcgcgg gcgaatcact tcgatatcgc
    13561 agccgacttc gccttcatca ctcaacagca gagcgatatc gtcaccgcta tggcttaagt
    13621 tgaaccatag cggcgtttcc ggcgcgaatg caggtttgcc ttgttcgcca tagatgatct
    13681 ccggtagcgg ggaaagcgtg tgcgaaagca atgcacgccc cgccagccag cgttcgcgtc
    13741 gtggaccttg cggtgcttgc tcgcgtaaac ccggtggcag tggagctgcg cttaaggtcg
    13801 aaactttccc cagaactatc cgatacatat cagggccaac gtttaatgga aaatgaaagt
    13861 gcgtatcgta tcacttgtcg cctcatcccg gtaaccgact tttcggtctg cccggcccca
    13921 gtaaaatcgc cagtttgctg ccacctttcg tcgtttccat ccagatttta cacacgctgg
    13981 ttaatggcac cgaaagtagc atccctaccg ggccgagcag ccatccccag attaacaatg
    14041 aaagaaatac caccatggtg gacatcccca ggcgatggcc catcatccgt ggctctaaaa
    14101 tattgccgat gaccatatgg acaactaaaa acaatgcgcc gaccagaata cattcgtaaa
    14161 taccattaaa cagcagcacc tgaatcattg gcggtacggc ggaaattacc gcgccgatat
    14221 tgggcacgta gttaagcaaa aacgccagta ctgcccacat cagcgcaaac tgtaccccca
    14281 tcagctccag ccccagccag acgatgacac ctgtccataa actgagtagc gtcttcaatg
    14341 caagatagtg cgaaacgcct ttaagtgcgc ggtgtaatcc cgcgatgtga atctgtgggt
    14401 tattcagcgc aaaacgcatt ttgtaaggga cgtggcgcac ttcaaacagc ataaaaacta
    14461 cggtcatcac cagcaaaagc acgctcgcca ttgccccgga aagcccggtc attagcgctg
    14521 tggtgaaggt aaccactttt tccgagtcca tccgctgcag cattcgctcc ggcgacatat
    14581 gcaaattaag aaaaggcaac atctcctgca atttaaaaag tttgcgcgtc aactccttat
    14641 taaacttcgg cagcatagag ataaattcgt taaacgatgc cgccagtacg ccgaccagcg
    14701 cagttagtgc gatcagcatc accaccacca caatcgtaat ggcaatgggg cgttgtactc
    14761 cccgacgaat aaaccaggtg acgagcgggt tgaggacgat ggcaaaaaac agcgccagta
    14821 acagctgcac aatgatatct gccgctgcgt gaatgcccgc gaggatcact accagcgagg
    14881 ccagcttgag cagaatgtgc atgcccgttt tatcgggttg aggggtttcc attggggctt
    14941 ccttgtgact ttttgtatta agtgtagcgg gagtcacgcc agcaatattc tgattcttac
    15001 tgctgatttt cacgtcagca cgtggtaaaa atgaaacact gttgtaaaaa tgtggtgatc
    15061 ctcatgcccg aacccgtagc cgaacccgcg ctaaacggat tgcgcctgaa tttgcgcatt
    15121 gtctctatag tcatgtttaa cttcgccagc tacctcacca tcgggttgcc gctcgctgta
    15181 ttaccgggct atgtccatga tgtgatgggc tttagcgctt tctgggcagg attggttatc
    15241 agcctgcaat atttcgccac cttgctgagc cgtcctcatg ccggacgtta cgccgatttg
    15301 ctgggcccca aaaagattgt cgtcttcggt ttatgcggct gctttttgag cggtctggga
    15361 tatctgacgg caggattaac cgccagtctg cccgtcatca gcctgttatt actttgcctg
    15421 ggacgcgtga tccttgggat tgggcaaagt tttgccggaa cgggatcgac cctgtggggc
    15481 gttggcgtgg ttggctcgct gcatatcggg cgggtgattt cgtggaacgg cattgtcact
    15541 tacggggcga tggcgatggg tgcgccgtta ggcgtcgtgt tttatcactg gggcggcttg
    15601 caggcgttag cgttaatcat tatgggcgtg gcgctggtgg ccattttgtt ggcgatcccg
    15661 cgtccgacgg taaaagccag taaaggcaaa ccgctgccgt ttcgcgcggt gcttgggagc
    15721 gtctggctgt acggtatggc gctggcactg gcttccgccg gatttggcgt catcgccacc
    15781 tttatcacgc tgttttatga cgctaaaggt tgggacggtg cggctttcgc gctgacgctg
    15841 tttagctgtg cgtttgtcgg tacgcgtttg ttattcccta acggcattaa ccgtatcggt
    15901 ggcttaaacg tagcgatgat ttgctttagc gttgagataa tcggcctgct actggttggc
    15961 gtggcgacta tgccgtggat ggcgaaaatc ggcgtcttac tggcgggggc cgggttttcg
    16021 ctggtgttcc cggcattggg tgtagtggcg gtaaaagcgg ttccgcagca aaatcagggg
    16081 gcggcgctgg caacttacac cgtatttatg gatttatcgc ttggcgtgac tggaccactg
    16141 gctgggctgg tgatgagctg ggcgggcgta ccggtgattt atctggcggc ggcgggactg
    16201 gtcgcaatcg cgttattact gacgtggcga ttaaaaaaac ggcctccgga acacgtccct
    16261 gaggccgcct catcatctta aaacttactg aataaccagc gtattaatga tgttttctgc
    16321 ggtggtctgc gctttttgct gatcgtcagc gggcagcgta atttgcatgg tcagcagttg
    16381 attacccacg ttacccagaa taacggaaga gtacgccgtc tggcctttcg cggagataat
    16441 actgtctaac tgctgcattt tgtgaccttt cagctcaatg gctttattgg ttaccacttg
    16501 cagctgcgga tcgcggctac gttgctgatc ttccagacgc ttcgccagca ccgccagatc
    16561 ttctttcgga tcatcgccca tgatgacgat gactgctttc tgcccggtgg cgtcggacca
    16621 gacatgcatg ttattggcct gcgttcccag cttaccgctc tggtcggtca tatccgctgg
    16681 cagcgagaaa cttaacttgc catcaagcag gttgacggga ttcccggtag cgttactttc
    16741 cgcgaccgaa ccctgcgccg tagcgttagt gtctttatca tcacaggccg caagccccat
    16801 aaccagcagg ccaattccga catatttaac cagattgcgc attgacttct tcctttcgat
    16861 aaacggccat aacggctcat tcatccatct tatcacaact ctgataacga acctttaact
    16921 cgcctgcaaa gcgttgattt cggatttatc tgccagtctt ttcaacagca tattgagtaa
    16981 tacgccatac attggcagga agaaaacgat actgattaac actttgaaac agtaatcgac
    17041 cagcgcgatt tccatccagt gttcagccat aaaggcatcc gggctacgcc agaaggcaat
    17101 gaagaaaaag gccagcgtgt cactgacgtt accgaacagt gtggacgctg tcggtgccag
    17161 ccaccagcgg cgactttgac gcaggcggtt aaaaacgtgc acgtcgagga tttgccccag
    17221 cgcgtaggcc atgaaactgg cggtggcgat acgggcgaca aacaggttga agtgggcgag
    17281 tgcgccgaat ccctgccatg aacccatata gaacagcgac gagatgacat aggagattaa
    17341 taacgcaggg atcattaccg cgaagataat gcgtcgggcc agcggtgcgc caaaaatacg
    17401 cacggtcagg tcggtcgcga gaaagataaa cggaaaacta aacgcgcccc aggtggtatg
    17461 aaaaccaaaa atggagaccg gaagctggac cagatagtta ctggaggtga tcaccagcag
    17521 atgaaataac gataaccaga acaacgcctt atagcgttga gtttgcgaga aaacgttcat
    17581 attgtacctt tttgattaac cattggggtg agggaaccca atacgtacga cacgtctgtt
    17641 atcttaacat aataacgtgc ttaccctttt ttcgagccgc cgcatgatac tgctttacgt
    17701 tgacaatgca atggttaatt ttcacgcaat cgttaacctg gtttgcttac ggactcacag
    17761 ggcgtaaact agcgccgttt ttttaagtga tgagaagaaa atgaccgatc tcttttccag
    17821 ccctgaccac acactcgacg cgcttggcct gcgctgcccg gaaccggtga tgatggtgcg
    17881 caaaaccgtg cgcaatatgc agcctggcga aactttactg attatcgccg acgatccggc
    17941 gacgacccgc gatattcctg ggttttgtac ctttatggaa cacgaactgg ttgcgaaaga
    18001 gacggatgga ctgccttatc gttatttgat tcgtaaaggc ggttgatggg gaaagattgg
    18061 cttcgatgcc gccttttccc ctcaccctaa ccctctcccc agaggagcga ggggaccgat
    18121 cgcgctcaat gttgcgatcg gtttgcctta tctcctgcgc aacaatctta acgcattcgc
    18181 tgttaccagc acggtcgcac cggtatctgc cagcaccgcc agccatagtc cggtcatccc
    18241 tagcagcgtg gtgacgagga agatcccttt cagccccagc gcaatagtga tgttctggcg
    18301 gatattggcg tgagtggcgc gggccagttc aatcatttgc accaggccgc gcaggtggtt
    18361 atgggttaat gctgcgtcgg cggtttccag cgccacgtct gtgccactgc ccattgcaat
    18421 cccaatggcg gcagctttca tcgctggcgc gtcgttaata ccatcaccga ccatcgccag
    18481 gggcgcatgt tgattcagct tggtcaccgc tttgacttta tcttccggca ataggcccgc
    18541 tttaaactcc agccccagct ccccggcaat tgccgccgct gcgcgtggat tatcgccggt
    18601 gaggatcact cctttaaccc ccagcgcgtt cagttcactg atggcagttg ctgcgtcggc
    18661 gcgcagggta tcctgcaatg caatgatacc cagcacgtca tcattacgta ctaccagcac
    18721 aaccgtttgc ccggcgcttt ccagttcatt aatcaaacca gcaaatgcat cagcgggatg
    18781 tttcccggca gcgcaaatca atacgcgctc accgttaacc tgcgcttcaa tgccagaccc
    18841 gaccagcgcc cgctgtgatt cggcggtggg aatggcgagt tcagcaacct gtgcttcgcg
    18901 tacgatggcc tgcgccagtg gatgcgtcgc gccttgctcg accgccgccg ccagtgtcag
    18961 cagttcagat tcactaatac ccgttgccgg atgaatcgcg gtaacgcgcg gtttaccgac
    19021 ggtcagcgta ccggttttat caaacgccac ctgagtaaca cgacccagct gttccagcgc
    19081 cgctccgcct ttaatcaacg ccccacgacg cgctgccgcc gccagcccgg aggtaatcgc
    19141 cgcaggcgtg gagataacta acgcacacgg gcagccaatc agcagcagcg tcagcccttt
    19201 ataaatccac tcctgccagc tggcggcaaa cagcagcggt ggcaccagcg tcaccagcag
    19261 ggcgacggcc ataatcgctg gcgtataaat acggctgaaa cggtcgataa accgctcaat
    19321 gggggcgcgg cgctcttcgg cttcttcgat cagtttcaga atccggtcaa tggcgctggc
    19381 acccggttct gacagcactt ccagcgttac cagacggtct acgctggtgg ctcctgcagg
    19441 aactttatcg cccgtcgcgc gctccaccgg aatggattct ccggtcaggg cgctttcatc
    19501 aaaactggca aacggtgaga gcagtttacc gtcggcaggc aaacgcccac ctgcggcgac
    19561 ttcaatcaca ttgccaggcc gcaggctgtt aatcgccacc tcttcccgct caccgttacg
    19621 caggcgcgtg gcggtttctg gtttcagcgc cattaacgcg ctaacgccct gacgcgcgcg
    19681 gctggcggcc cagccttcca gtcgttcacc aatcaaaaac agcagcaaca ccatcgcagc
    19741 ttcagccgtt gcgccaataa acagtgcacc aatagcggct acgctcatta aggtttcaat
    19801 ggcgaagtag ctgccggatt tgatcagccg taatgcctga cgagcaatcg ggtacagccc
    19861 aaccagcgtg gtcgcgataa acgccagttg cccaaacgga tgattaaatt gctccagacc
    19921 ccagctgatt gccatcatca cgattagcgt aatcagcggc agattctctt tcaggcgcga
    19981 tgcttgtggc tcgtcggcgg cctgctcatc gcgcagggaa tagcccgctt tttgcaccgc
    20041 agattcaacc tgggcacgga tgtcattgtc ggcatcgacc accagttttt cggtggcgaa
    20101 cagtacctgc acctgattca cacctgcaag ctggcgcacg gcattttcta ccttgcgcgc
    20161 acaggcggcg cagtccatgc cgctgacttt ccagctatag cgggtgccgg agacgttttc
    20221 agagagcgtt ggcgagctgg aacatgcgcc gtcgcagcaa cagtcgttgg cgttctgtac
    20281 cgtggttagc ggtttgaacg cagcaaattg aggggctttc ttgccgtgat tgtcaggagt
    20341 cgacatggca tcctccggtt aagttttttc tcattaaccg aaggatacac tctggagtcg
    20401 actccagagt caagttttat cagagataca gcgagcggac gatgaggaaa tgaccggcaa
    20461 agtagcaggc cgcagcaatc gcgttatctg cccggaagcg gcggcgatag tggctaccca
    20521 gccagacaaa gttactgata aacagcaatg aagcgccaac aaacgcagag agtgccggag
    20581 cggtcggacg gaagaaccac agttcacctg ccagccacac catcaccagc gtcatgccga
    20641 taaacgtgca gataggccaa cgatactctt ccagccgcgt ccagataatc gccagtaaca
    20701 gcgcgcccag aaccagtaac accagtggca gaggccagaa gaaagagagc gtcatctgac
    20761 tggcgaaata gatggtgtac agcaggtgcg agaggaaaaa cgcgccgatg gcgtacatca
    20821 gacgttgacg tggcaacagg gttagcgcat cgcccagcag tgaggcgcac agccctgcca
    20881 gcaccagata gctaatggcg tcgaacatcg gcgcttgcca ggccagtaac agcaggagaa
    20941 gaagggttaa cggtttaaac acccagcgtt gccaggttgg cccacgatac gatgcatcca
    21001 cagatagcca tgtggaaaga cagacagcga taaacgacca aagcatctta gttccttgaa
    21061 ttcgttattt tttcgtaagc ggaatgccta cggtcttgct tccagtttag tggcgagggt
    21121 ggacggatga caacaccata actggcaggg tatgcttatt tccgcatttt ccgatgaggg
    21181 ataaagatga gcaaaccacc tcttttcttt attgttatca ttggcttaat tgtcgtcgcc
    21241 gcatcgtttc gttttatgca acagcgacgg gaaaaagctg ataatgatat ggctccgctc
    21301 cagcaaaagc tggtggtggt gagcaacaag cgggaaaaac cgattaacga tcgccgttcg
    21361 cgccagcagg aagtgactcc ggcaggcacc agtatgcgct atgaggcaag cttcaaaccg
    21421 caaagcggag gaatggagca gacgtttcgc ctcgacgccc agcagtacca cgccctgaca
    21481 gtgggcgata aaggtacgct gagctataaa ggaacgcgct ttgtcagctt tgtaggcgaa
    21541 caataaggtt attttttaac tttaaatttc ttctgccaga ccagcagttc aaacacgccg
    21601 aaaaggaaaa tccgcacctt ttcggcggtg gtcatctgcg ggccatcttt cggtaaagtg
    21661 gatttcaata gcgccagttg cataccatgc atcagcacgg taaaaatcag cgccacgtta
    21721 acgaaaatat tcagcgggcg cgggaaggga tgcaccagat tgaggattaa aaatccccaa
    21781 acgcagagca ttaacaaacg accaatatta atcagcatca ctttctcctt gtgcttcgcg
    21841 ttgatacagc cgataagcca cctgacccgc cactttttcc cgatgtaatg accagtttgc
    21901 cggaacagtg ggcagaccgt tttcgacttc gctttcgaca taaatcaggg cttcgtcagc
    21961 cagccagccg ttatcttcca gtaaatttat cgtctcttct aacaagccac ggcggaacgg
    22021 tggatcgaca aacacgatat tatgcggtgt gcctttttgc gccaggaatg acattgcgtt
    22081 gctgttcacc acgcgtgcat tgcctgcttt tagtgtcgcc agattcttaa ttaactgctg
    22141 agaaactgcg cgatccatct caatcaacgt ggcccccgca gcgtagcgcg ataacgcttc
    22201 cagccccagc gcgccgctcc cggcgaagca atccagacat tgggcgtcaa caatgaccgg
    22261 agccagccag ttaaacaacg tttcgcgtac gcggtcggtg gtggggcgca gacctgggct
    22321 atcaggaacc gggagtttac ggcctcgcca ctgcccgcca ataatgcgga tttggccgct
    22381 gccggaatga ttcggttttt tcatgataaa ttgctcaatc cgccagataa cacatacttg
    22441 caggcggtga tgtgaattaa gttaagtgat agactatttc atcatttttt tagctgctat
    22501 gtacatagcg ttaacgctgt gccatgaagc aacagcgagg agtgtagtcg caaatggcga
    22561 aagaaaaaaa acgtggcttt ttttcctggc tgggctttgg tcaaaaagag cagaccccgg
    22621 aaaaagagac agaagttcag aatgaacaac cggttgtaga agaaatcgtt caggcgcaag
    22681 agcctgtgaa ggcttctgaa caagccgtcg aagagcagcc gcaggcgcat actgaagccg
    22741 aggcggaaac ttttgctgcc gacgttgtgg aagtcactga acaggttgct gaaagtgaaa
    22801 aagcgcagcc tgaagcggaa gtcgttgcac agccggaacc ggtcgtagaa gaaacgccgg
    22861 agccagtggc tatcgagcgt gaagagctgc cgttgccgga agacgtcaac gccgaagagg
    22921 tttcgccaga agagtggcag gctgaagcgg aaaccgtaga gattgtcgaa gcggcggaag
    22981 aagaagcggc taaagaagaa atcaccgacg aagagctgga agcacaggcg ctggctgccg
    23041 aagcggcaga agaggcggtg atggtggttc ctccggtaga agagcagccg gtggaagaaa
    23101 tcgctcagga gcaggaaaaa ccgaccaaag aaggtttctt cgcgcgcctg aaacgcagcc
    23161 tgttaaaaac caaagaaaat ctcggttccg gatttatcag cctgttccgc ggtaaaaaaa
    23221 tcgacgatga tctgtttgag gagctggaag aacagctgtt gatcgccgat gtgggcgtgg
    23281 aaaccacacg taaaattatc accaatctga cggaaggcgc atcccgcaag cagcttcgtg
    23341 atgccgaggc gctctatggc ctgctgaaag aagagatggg cgagattctg gcgaaagtcg
    23401 atgagccgct gaatgttgaa ggtaaaacgc cgttcgtgat cctgatggtg ggcgtcaacg
    23461 gtgtgggtaa aaccacgacg attggtaagc tggcgcgtca gtttgagcag cagggtaaat
    23521 cggtgatgct ggcggcgggc gatactttcc gtgcagcagc ggttgaacag cttcaggtct
    23581 ggggtcagcg caacaatatt ccggtgattg cccagcatac tggtgcggat tccgcctctg
    23641 ttatcttcga cgccattcag gcagctaaag cgcgtaatat cgacgtcctg attgccgata
    23701 ccgccggacg tctgcagaac aaatcgcatc tgatggaaga gttgaagaaa atcgtccgcg
    23761 tgatgaagaa actcgacgtt gaagcgccgc atgaagttat gctgactatt gatgccagca
    23821 ccgggcagaa cgcggtaagc caggccaaac tgttccacga agctgttggc ttaaccggca
    23881 tcacgctaac gaaactggac ggcacggcga aaggcggggt aattttctcg gtggctgacc
    23941 agtttggtat ccctatccgc tacattggtg tcggcgaacg tattgaggat ttgcgtccgt
    24001 ttaaggcgga cgactttata gaggcacttt ttgcccgaga ggattaacaa tgattcgctt
    24061 tgaacatgtc agcaaggctt atctcggtgg gagacaggcg ctgcagggcg ttacgttcca
    24121 tatgcagccg ggtgagatgg cgtttctgac cggtcattcc ggcgcaggga aaagtaccct
    24181 cctgaagctg atctgtggga ttgagcggcc cagcgccggg aaaatctggt ttagcggcca
    24241 tgacatcacg cgtctgaaaa accgtgaagt tccgtttctg cgccgccaga ttggcatgat
    24301 tttccaggat caccatctac tgatggaccg tactgtctac gataacgtgg cgatcccgct
    24361 gattatcgct ggtgccagcg gtgacgatat tcgtcgccgg gtgtcggcgg cactggataa
    24421 agtcgggcta ctggacaaag cgaagaactt ccctattcag ctttccggcg gtgaacaaca
    24481 gcgtgttggc attgcccgcg cggtggtgaa taagcccgcg gtactgctgg cggacgaacc
    24541 gaccggtaac ctggacgacg cgttgtcgga agggattcta cgtctgtttg aagagtttaa
    24601 ccgcgttggg gtaaccgtat tgatggcaac gcacgacatc aacctgattt cacggcgttc
    24661 ctatcgcatg ctcaccctga gcgatggtca cttgcatgga ggcgtgggcc atgaataagc
    24721 gcgatgcaat caatcatatt cggcagtttg gtgggcgtct ggatcgcttc cgtaaatcgg
    24781 tcggcggctc aggcgacggc ggtcgtaacg caccgaaacg cgcgaaatcc tcgccaaaac
    24841 cggtaaatcg caaaaccaac gttttcaacg aacaggtgcg ctatgccttc cacggcgcat
    24901 tgcaggatct gaaaagcaaa ccgttcgcca cgtttttaac ggtgatggtt atcgccatct
    24961 ccctgacgct gcccagcgtc tgttatatgg tgtacaaaaa cgttaatcag gcggcgacgc
    25021 agtattatcc gtcaccgcaa atcactgttt acttgcaaaa aacgctggac gatgacgccg
    25081 ctgcgggcgt ggtggcacag ttgcaggccg agcaaggcgt ggagaaagtg aactatcttt
    25141 ctcgtgaaga cgcactgggc gagttccgta actggtctgg ttttggtggt gcgctggata
    25201 tgctggaaga aaacccgctt ccggcagtgg cggtggtgat cccgaaactc gattttcagg
    25261 ggacggaatc actcaatacg ctgcgtgatc gtatcacgca gattaacggc attgacgaag
    25321 tgcggatgga tgacagctgg tttgcccgtc tggcggcgtt gaccgggctg gtcgggcgcg
    25381 tttcggcgat gatcggcgtg ttgatggtgg cggcagtgtt cctcgtcatc ggcaacagcg
    25441 tacgtctgag catctttgct cgccgtgact ccatcaacgt gcagaaactg attggtgcga
    25501 cggatggttt catccttcgc ccgttcctgt atggtggcgc actgctggga ttttctggcg
    25561 cattgttgtc attaattttg tcagaaattc tggtgctgcg attgtcatcg gcggttgcgg
    25621 aagtggcaca ggttttcgga acgaagtttg atatcaatgg tttatcgttc gatgaatgcc
    25681 tgctattgct gctggtatgc tcgatgattg gctgggtggc agcgtggctt gccacggtac
    25741 aacatttacg ccactttacg cctgaataat aaaagcgtgt tatactcttt ccctgcaatg
    25801 ggttccgtag cagggaaaga gatcccgttg tctcttcccg gtatttcatc tctatgtcac
    25861 attttgtgcg taatttattc acaagcttgc attgaacttg tggataaaat cacggtctga
    25921 taaaacagtg aatgataacc tcgttgctct taagctctgg cacagttgtt gctaccactg
    25981 aagcgccaga agatatcgat tgagaggatt tgaatgactg acaaaatgca aagtttagct
    26041 ttagccccag ttggcaacct ggattcctac atccgggcag ctaacgcgtg gccgatgttg
    26101 tcggctgacg aggagcgggc gctggctgaa aagctgcatt accatggcga tctggaagca
    26161 gctaaaacgc tgatcctgtc tcacctgcgg tttgttgttc atattgctcg taattatgcg
    26221 ggctatggcc tgccacaggc ggatttgatt caggaaggta acatcggcct gatgaaagca
    26281 gtgcgccgtt tcaacccgga agtgggtgtg cgcctggtct ccttcgccgt tcactggatc
    26341 aaagcagaga tccacgaata cgttctgcgt aactggcgta tcgtcaaagt tgcgaccacc
    26401 aaagcgcagc gcaaactgtt cttcaacctg cgtaaaacca agcagcgtct gggctggttt
    26461 aaccaggatg aagtcgaaat ggtggcccgt gaactgggcg taaccagcaa agacgttcgt
    26521 gagatggaat cacgtatggc ggcacaggac atgacctttg acctgtcttc cgacgacgat
    26581 tccgacagcc agccgatggc accggtgctc tatctgcagg ataaatcatc taactttgcc
    26641 gacggcatcg aagatgataa ctgggaagag caggcggcaa accgtctgac cgacgcgatg
    26701 cagggtctgg acgaacgcag ccaggacatc atccgtgcgc gctggctgga cgaagacaac
    26761 aagtccacgt tgcaggaact ggctgaccgt tacggtgttt ccgccgagcg tgtgcgccag
    26821 ctggaaaaga acgcgatgaa aaaacttcgc gctgccattg aagcgtaatt tctactacta
    26881 agcagagaac cctggatgag aatccggggt ttttgttttt tgggcctctg caataatcag
    26941 tttccccctc cggcaaaacg ccaatcccca cgcagattgt taataaactg tcaaaatagc
    27001 tattccaata tcataaaaat cgggtatgtt ttagcagagt atgctgctaa agcacgggta
    27061 gtcatgcata aaacgaaata aagtgctgaa caacaacatc acaacacacg taataaccag
    27121 aagaatgggg aacctcagga tgaacatgaa gggtaaagcg ttactggcag gatgtatcgc
    27181 gctggcattc agcaatatgg ctctggcaga agatattaaa gtcgcggtcg tgggcgcaat
    27241 gtccggtccg gtggcccagt acggtgacca ggagtttact ggcgcagagc aggcggttgc
    27301 ggatatcaac gctaaaggcg gcattaaagg caacaaactg caaatcgtga aatatgacga
    27361 tgcctgtgac ccaaaacagg cggttgcggt ggcgaacaaa gtcgttaacg acggcattaa
    27421 atatgtgatt ggtcacctct gttcttcatc aacgcagcca gcgtcagaca tctatgaaga
    27481 tgaaggcatt ttaatgatca ccccagcggc aaccgcacca gagctgaccg ctcgtggcta
    27541 tcagctgatc ctgcgcacca ccggcctgga ctccgaccag gggccgacgg cggcgaaata
    27601 tattcttgag aaggtgaaac cgcagcgtat tgctatcgtc cacgacaaac agcagtacgg
    27661 cgaaggtctg gcgcgtgcgg tccaggacgg cctgaagaaa ggcaatgcta acgtggtgtt
    27721 ctttgatggc atcaccgccg gggaaaaaga tttctctacg ctggtggcgc gtctgaaaaa
    27781 agagaatatc gacttcgttt actacggcgg ttatcacccg gaaatggggc agatcctgcg
    27841 tcaggcacgc gcggcagggc tgaaaactca gtttatgggg ccggaaggtg tggctaacgt
    27901 ttcgctgtct aacattgcgg gcgaatcagc ggaagggctg ctggtgacca agccgaagaa
    27961 ctacgatcag gttccggcga acaaacccat tgttgacgcg atcaaagcga aaaaacagga
    28021 cccaagcggc gcgttcgtct ggaccaccta cgccgcgctg caatctttgc aggcgggcct
    28081 gaatcagtct gacgatccgg ctgaaatcgc caaatacctg aaagcgaact cggttgatac
    28141 cgtgatgggg ccgctgacgt gggatgagaa aggcgatctg aaaggctttg agttcggcgt
    28201 atttgactgg cacgccaacg gcacggcaac cgatgcgaag taacagatag ataaatgaaa
    28261 atgcccgatg cgctgaactg ccatcgggtt tttttgtgtt tgtcgaaatg gttgtagaaa
    28321 gattaaacaa tccaggatat gcttaatgta cggtgacata ccgtacatat tatacggagc
    28381 aggagagttc acatgtctgc tgttaaaaag cagcgtatcg atctgcgttt aactgacgat
    28441 gacaaaagta tgattgagga agcggcagcg atatctaatc agtccgtcag ccaatttatg
    28501 ttgaacagcg cttcgcagcg ggctgcggaa gtgattgaac agcatcggcg ggtgatcctc
    28561 aatgaggaat cctggacgcg ggtgatggat gcgctgagta atccaccgtc acctggtgaa
    28621 aagctaaaac gtgcggcaaa acgtcttcag ggaatgtaat aatttatgga tgatctgacg
    28681 atagagattc tgaccgatga tgcagattat gatctacagc gattcgactg cggcgaggaa
    28741 gcgttaaatc tctttctgac gacacatctc gttcgtcaac atcgcaacaa aattctgcga
    28801 gcgtatatcc tttgtcgcaa cactccagaa cgtcaggtgc tgggatatta cacattatgc
    28861 ggcagttgtt ttgaacgagc cgcattgccc tcgaaatcga aacagaaaaa aattccctac
    28921 aaaaatattc ccagcgttac tcttgggcgt ctggcaattg atcgttcatt acaggggcag
    28981 ggatggggag caacactggt tgctcatgcc atgaaagtcg tctggtcagc ctctttagcg
    29041 gtaggtattc acggtctttt tgtcgaggcg ctgaataaaa aagcccatac gttttatcaa
    29101 tcgctgggct ttatcccgtt ggtcggagaa aacgaaaatg cgttattttt cccaaccaaa
    29161 tccatcgaac tgcttttcac acagagcgat taacgcttct cccagccgcc ctgttgcgcc
    29221 gtaaatccca gtgcctgcat aaacgccgtc atcacaccgc gatcttccac gcctgcatcc
    29281 gccatccacc agcatgaaac gccaggattg ttacgcaaaa cctcttccag cagatattgc
    29341 cccacaccgc gacggcgggt gacttcccgc acgcacaggg aatccagtgc tccctcggtg
    29401 ccgcttaagg tcacccgcac ggccgctagc aggcgctcgt taaaacgcgc ggcgtagata
    29461 cggtggttat cgtcaacctg taaagaggaa ggggaatact ccggccagat cttttgcagg
    29521 tcaatccggt cctggtcgct aaagttttct aatcgaatga tggtcagctt catggggaac
    29581 ccgtgtaaat cacaaaagtg gaaccagtgt agcgaaataa tttaatcgga ggctttctct
    29641 tttttatttc ttttggcagg tga
//
LOCUS       DBANUU010000054        28348 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000054 DBANUU010000000
VERSION     DBANUU010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.054"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            109..315
                     /locus_tag="ACIE68_002270"
                     /pseudo
     CDS             109..315
                     /locus_tag="ACIE68_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            311..663
                     /gene="prfH"
                     /locus_tag="ACIE68_002271"
                     /pseudo
     CDS             311..663
                     /gene="prfH"
                     /locus_tag="ACIE68_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(720..2177)
                     /gene="pepD"
                     /locus_tag="ACIE68_002272"
     CDS             complement(720..2177)
                     /gene="pepD"
                     /locus_tag="ACIE68_002272"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFX5908581.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQDILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIKSVGHYWTLLTELLKEIPAK"
     gene            2438..2896
                     /gene="gpt"
                     /locus_tag="ACIE68_002273"
     CDS             2438..2896
                     /gene="gpt"
                     /locus_tag="ACIE68_002273"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFX5908582.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDNYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2988..4232
                     /gene="frsA"
                     /locus_tag="ACIE68_002274"
     CDS             2988..4232
                     /gene="frsA"
                     /locus_tag="ACIE68_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFX5908583.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4290..4691
                     /gene="crl"
                     /locus_tag="ACIE68_002275"
     CDS             4290..4691
                     /gene="crl"
                     /locus_tag="ACIE68_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFX5908584.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(4730..5785)
                     /gene="phoE"
                     /locus_tag="ACIE68_002276"
     CDS             complement(4730..5785)
                     /gene="phoE"
                     /locus_tag="ACIE68_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFX5908585.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6073..7176
                     /gene="proB"
                     /locus_tag="ACIE68_002277"
     CDS             6073..7176
                     /gene="proB"
                     /locus_tag="ACIE68_002277"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFX5908586.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7188..8441
                     /gene="proA"
                     /locus_tag="ACIE68_002278"
     CDS             7188..8441
                     /gene="proA"
                     /locus_tag="ACIE68_002278"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFX5908587.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPVGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MEESGVALHADAAALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            8556..8631
                     /locus_tag="ACIE68_002279"
     tRNA            8556..8631
                     /locus_tag="ACIE68_002279"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8589..8591,aa:Thr,seq:cgt)
     gene            8797..10011
                     /locus_tag="ACIE68_002280"
     CDS             8797..10011
                     /locus_tag="ACIE68_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFX5908588.1"
                     /translation="MKLNARQIDTAKPKEKAYKLADGGGLYLLVKPNGGKYWRLKYRV
                     AGKEKLLALGVYPEVTLADARAKREDAKRGIAGGIDPMEAKREEKIARETQLNNTFKD
                     IALEWHSSKLKKWSAGYASDILEAFNKDVFPYIGKKPIAEIKPLELLNVLRRIEGRGA
                     TEKAKKVRQRCGEVFRYAIVTGRAEYNPAPDLTSAMQGHESNHYPFLTAKELPDFFKA
                     LSSYSGSALVVMAARLLIITGLRTGELRGALWDEIDFNKAIWEIPASRMKMRRPHIVP
                     LSEQALSLIGKIREITGNYPLMFPGRNDPRKTMSEASINQVFKRIGYAGRVTGHGFRH
                     TMSTILHEQGYNTAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLEHG
                     GNVVHMVFEKHA"
     gene            10440..10643
                     /locus_tag="ACIE68_002281"
     CDS             10440..10643
                     /locus_tag="ACIE68_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFX5908589.1"
                     /translation="MANYNSLIRLSEVQRRTGYSKAWIYRLISQGRFPKQVKIGSRAI
                     AFVESEIDEWIEKCILESRDEVA"
     gene            10643..11074
                     /locus_tag="ACIE68_002282"
     CDS             10643..11074
                     /locus_tag="ACIE68_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908590.1"
                     /translation="MEKKNRPLQAANSDIRVSDVTPLTKSPQAPKRTPKKHRARVYML
                     RTGIEGWTENDILRYCRLSSGRNYATELERQLGITLERIDEKNPDGIGTHLRYRFSCR
                     GDVLKVITHINHLANINDHNGLSQQEIADILKLYPDAFNAA"
     gene            11087..11920
                     /locus_tag="ACIE68_002283"
     CDS             11087..11920
                     /locus_tag="ACIE68_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFX5908591.1"
                     /translation="MNIEKSRLISEAAPHLNASLGTINGNEFAAIVPVIPGHIGGRET
                     NIVSAKALHKALGVGKDFSTWITDRISEYDFTIGHDYSVHKTISPNLGKSPNGAAYSK
                     IKHSGRPGKDYLLSVGMAKELAMIERNDQGRAIRRYFIQCEEELQRSVPEIAARYRRQ
                     LKARISAANNFKPMCDALNMARAEMGKTTQQHHYTNESNMISRIVLGGLTAKQWARIN
                     GYSGEPRDHMNAEQLEHLSYLESTNITLIDMGMEYEQRKGELTRLSQRWLAKRLEALN
                     V"
     gene            11913..12092
                     /locus_tag="ACIE68_002284"
     CDS             11913..12092
                     /locus_tag="ACIE68_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFX5908592.1"
                     /translation="MSKPTGIPQPQKRYKDAHGALVTVESVSHNRVTFYRDGYQSPCV
                     QPLARFMKEFAEVNQ"
     gene            complement(12239..12328)
                     /locus_tag="ACIE68_002285"
                     /pseudo
     CDS             complement(12239..12328)
                     /locus_tag="ACIE68_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729926.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
     gene            12377..12826
                     /locus_tag="ACIE68_002286"
     CDS             12377..12826
                     /locus_tag="ACIE68_002286"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFX5908593.1"
                     /translation="MYCASGASNSEFSESIKKSLPLCGNTVYGYKAPHKTGAGIGVLK
                     ILKATYDAPSVFFYVVGLTHPFFGRWCIIQRLCQSMVAQAGASSEAPVSFRAGYANPV
                     WATTSEIGVSGGSVTCYLKEAATCWLLPLPKNRNLSGLSPQFAAIAR"
     gene            12751..12945
                     /locus_tag="ACIE68_002287"
     CDS             12751..12945
                     /locus_tag="ACIE68_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFX5908594.1"
                     /translation="MLATTPTQKPQFIWIIAAVRRDCPIITAKIHHIAAESERDARRS
                     LVRDHVCFFAGRIRMEVAHD"
     gene            12938..13156
                     /locus_tag="ACIE68_002288"
     CDS             12938..13156
                     /locus_tag="ACIE68_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908595.1"
                     /translation="MIKTYDVHMDPLERTSQIITLTEVINDILVSNSPSRDEKLKALL
                     AILDLAVRDVHFLLEGGQMPCKTGATNE"
     gene            13149..13343
                     /locus_tag="ACIE68_002289"
     CDS             13149..13343
                     /locus_tag="ACIE68_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908596.1"
                     /translation="MNNSINTPRLTSALQLIEQAAAVLLAVSLSAEEMDAADVVDAIK
                     ACSSLVNDARAELVILGGEK"
     gene            13340..13603
                     /locus_tag="ACIE68_002290"
     CDS             13340..13603
                     /locus_tag="ACIE68_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinic acetylcholine receptor subunit beta"
                     /protein_id="HFX5908597.1"
                     /translation="MNINLIYRHPCELEIESLLGREEPYPDTFTPADCATERLTRART
                     GLVHVMNEIIPSVGGEQATVINSWLQKVTSLIDISLIDVESAK"
     gene            13600..13821
                     /locus_tag="ACIE68_002291"
     CDS             13600..13821
                     /locus_tag="ACIE68_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908598.1"
                     /translation="MTNIQLIEAQCRIEQVQTVLGFWLEGASPSNRDKLMIGAVMSLL
                     NGVPEAIQEADELLGKYELQNHSGEAKHE"
     gene            13814..14416
                     /locus_tag="ACIE68_002292"
     CDS             13814..14416
                     /locus_tag="ACIE68_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908599.1"
                     /translation="MNNFLTFHAEATPDGVNIMHRSNDGMTERVETVSYIDAVNRLDA
                     GDYDDKPDEGMSIHLAIADGGNQGYFDYTSQHNVIMWRWLIATVFMLEMREENGTVSI
                     IDDTGNPSEVAVYSNGIVAMPLYPVAERLAMANNIEGAMIERFGIESGTERSIIFYRA
                     MMDVEQGALTPFGRETLAELHNSFIAELNENGMPSEPVTH"
     gene            14427..14768
                     /locus_tag="ACIE68_002293"
     CDS             14427..14768
                     /locus_tag="ACIE68_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908600.1"
                     /translation="MITKNFRLNALANKYASALYNHITSTSGGDYFMVDADGEAVRVE
                     IVNGVKGVRSLIDSYTLAAMKVFYPQWETVGIELIERCVTKDGLTDVGREIWQSMVND
                     MGATVAGGSHA"
     gene            14761..15132
                     /locus_tag="ACIE68_002294"
     CDS             14761..15132
                     /locus_tag="ACIE68_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908601.1"
                     /translation="MRENAEMALSSAIGEQVAKIAGAVWIHNLHSTGEEKMAIQTPEG
                     RTITTSLKPSDVCDLICAFMYPAMRTVHGDKWKLATTAEFDMWLNNDGMLTDYGITKW
                     QMLVSHIANAIDHVGYGDAKH"
     gene            15119..17875
                     /locus_tag="ACIE68_002295"
     CDS             15119..17875
                     /locus_tag="ACIE68_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFX5908602.1"
                     /translation="MRNIDLIRQVISASENNWPHVLGCLNINVPDSPRRHAPCPACGG
                     KDRFRFDDNGRGSFICNQCGAGDGLDLIKRVNNCDTTEAALLAADVLGIDYRTTETPE
                     ATSQKREQLETERQRREQERLKRAEKDEQQRRDTFSRQFDDMRRKAVNGKSDYLVAKG
                     VGDFTFPVLPDGSLLLALVDKSGAVTAAQTITSHGEKRLLTGSAKRGAYHAINAPETT
                     QSILIAEGLATALSAHLMRPEALTVAAIDAGNLLYVAQVLRDKFPSAQIIIAADNDHS
                     EGRQNTGRIAAEKAALSVSGWVALPPTDHKADWNDYHQKHGIKCATEAFNKSMYQPQG
                     NGVKQEPQTIEGSDFKVMDTDPLKPRIESREDGIYWVSPRADSQSGEIINNESWLCSP
                     LSVIGTGRDDKDQYLILRWLSFGSETPTTAAIPLADIGEREGWRTLKAGGVNVTTKSS
                     LRAILADWLQRSGSRELWRVAHATGWQCGAYIMPDGEIIGTPENPVLFSGRSSAAAGY
                     TVSGSAKSWRDNVARLAFGNYSMMTGIGAALAAPLIGLVGADGFGIHFYEQSSAGKTT
                     TANVASSLYGNPDLLRLTWYGTALGLANEAAAHNDGLMPLDEVGQGADPVSVSQSAYA
                     LFNGVGKLQGAKDGGNRDLKRWRTVAISTGEMDLETFIATSGRKTKAGQLVRLLNIPL
                     SKAVRFHDYQNGKQHADALKDAYQHHHGASGREWIKWLADHQQQAIKTVRDCESRWRS
                     LIPSDYGEQVHRVAARFAILEAALLLGEVVTGWDAQTCRDAIQHSYNAWLREFGTGNK
                     EHQQIIEQTEAFLNAYGLSRFAPFPYSPADLPIKDLAGYRQRGEHDESPMIFYTFPAT
                     FEKEIACGFNAKQFAEVLKKAGMLTPPNSGRGYQRKSPRIQGRQINVYVLNYQPGDYN
                     SSEE"
     gene            18460..18621
                     /locus_tag="ACIE68_002296"
     CDS             18460..18621
                     /locus_tag="ACIE68_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908603.1"
                     /translation="MAISVKPVLISEKQMEAIKKIQEEQRKKSGIGVAPTLHEIARGL
                     IDKALAGCM"
     gene            18638..19099
                     /locus_tag="ACIE68_002297"
     CDS             18638..19099
                     /locus_tag="ACIE68_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFX5908604.1"
                     /translation="MELKFIDNPVRLQAFLNEPGNTENIVEPGHTYYIKPDAVYLGIY
                     EGLVLAGVHEVRNFWHSVVECHAVYDPGFRGEYALQGHRLFCKWLLENSPFLNSITMV
                     PDTTKYGRAIIRLLGATRVGHLDDAYIRCGKPVGVTLYQLTRQQYKEFSEC"
     gene            19093..19770
                     /locus_tag="ACIE68_002298"
     CDS             19093..19770
                     /locus_tag="ACIE68_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFX5908605.1"
                     /translation="MLITHIAHKHLNRAVYEKGGDGGASKAQAKAQQQAIDLQREQWN
                     TVMNNLKPYAEVGLPALQQLQGLMTLEGQNKAANDFFGSGLYKTQADQARYQNLVSAE
                     ATGGLGSTATSNQLSSIAPMLYNNWLSGQMQNYGNLLNVGMNAASGQATAGQNYANNT
                     GQLLQGLGAIRAGQAQQPSSLARGIGGAASGALAGAQLGSVVPGIGNVAGAIGGGLIG
                     LVGGLGF"
     gene            19803..21089
                     /locus_tag="ACIE68_002299"
     CDS             19803..21089
                     /locus_tag="ACIE68_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFX5908606.1"
                     /translation="MAPQNDNAPRSSVPQPVQYQQQPNVGLMALQGLRGVAEINQQAQ
                     ISQQLKEYQARLGSAVANNDRVGVQKLMAQYPQFMADSQKQMGFIDAEQNRQTADAAM
                     NLRLASQTGDPLTMQKAIQQANPVLQRFGLPAEEVYQSWQNDPQEFNRITDLIHLHAD
                     PNSYFSVQEKQQGQSIAQQNADTSAYNAQVNAEQGNRRLDMEQFKVISDNQLRTREMV
                     LKARLGRAQNDIERDKLNAELSKVQQAKVEKMNDKINTVAGNIDSLNSALEVGHELAG
                     IVGKHPSAVTRSQNYNPLTLGFGFLPDVAEDTRSLRAKADEYNLKVILPSLKGTFGSN
                     PTEGERAALMQSQNGIKSATSNEAFLRELNKAQDVIIRMQKRQIAGLGIPVTKSKDEE
                     TDTKMLLQDPSLVKDYVTAHGYLPESYYQAKLKGWQ"
     gene            21086..23806
                     /locus_tag="ACIE68_002300"
     CDS             21086..23806
                     /locus_tag="ACIE68_002300"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFX5908607.1"
                     /translation="MMAQLTEKQKAYSRHLDEKYGFPSGTMETLVGNESSGNGSAESP
                     KGARGFAQLMPSVMSDAGYRGTDPRSLPFEKQMDIAANHLNKGMKRFGDIGMALAGYN
                     SGYARINAVRNGKSTLPDETANYVSKFADAGIIPDDSEILQFAQTSQHRNLQQPDYSE
                     LDKEISQVGDISAWEAKRQQFKNIEAGLPTASSWEQKRAQFLADENAGEPQQQVAKSN
                     LNPEMEYLSPGLKAAPEQMARGIANIPFDVMDAGVGVVNAAQSAGAWAAQKLGVGDGT
                     YTPISPVFRPVDQPTDPYAQAGEQLAPYLITGLGAGKAATAASSVANAPRAERAATKV
                     ASMLAENLPGTLVNAQKNGQISPEDLAKETGIGLAGSLGARALIKSGQTASDVMRDVL
                     RSNTNQSGHVLGAANTLETANDVSKAARSAVGREHIASQASNVRDDVAKAAKITGINI
                     NTLTPGMRSGSRGIAQAEGVLASTPGNVQDAHLTAFNEISSKLNKQLSDFGAESGSAS
                     EKSAAIKERIIASLSDMKTAENMAWAELRSTMPRERMKMSNGMAVVQAEKAANVPLSP
                     EMKQFQAANNQGGGITFDGMKAWRAKFADAEQANTRAGKANAARIAGEMRRAITDDMR
                     IMAEKGNFLTEWQKANDLSKSYIIAKQNAESVFGRDLASDAMVRKGADTLKASANTGT
                     GAFHRLISALPEAERQPAIASILQHAVAQGEKGGIGEESGIQYFAKLMTQQNVNAISR
                     HAPDLGRMMKEFGVLAKASTRPQRYVERTGRTTEALKTLDSGLPKIVGVIMNGVQQSG
                     AIAGGFGGGVIGSISGLVAGGALRNTVAKIGATRSGRYAIEKAVQEATKAVRAGASPA
                     AIAAAEKRFSANKIAMKAIRDAVGAEEFVKLTRAGIVATLSGMKDVSEWE"
     gene            23957..24655
                     /locus_tag="ACIE68_002301"
     CDS             23957..24655
                     /locus_tag="ACIE68_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908608.1"
                     /translation="MIKNLLLILSLSLLAGCVQKPLVTVNALSSGQSVGKKFLIIPAS
                     QELWNTKQIEYTQIATVVAASLSSQGYTQVKDEKLADQVILLNFLRSGAISNTRNVTI
                     PIYGQTGVSSATTYGNSNTTFNSYGGGYGTANTTGTATTVYTPTYGITGAYNTTVTDT
                     FYGAGVILQSFDAKAYLNNRQINMLWKVGSSVVSQQSDGIADYKGLARIAASYAGKNT
                     EGDITFNVDPKAPN"
     gene            24721..25005
                     /locus_tag="ACIE68_002302"
     CDS             24721..25005
                     /locus_tag="ACIE68_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908609.1"
                     /translation="MRQTLSDIAYTFGAGFQIRSQLMGGVTVRAYPLYVKQEDFSDIT
                     ATAKHQFRDQYAREGYQLQKILQLRPTSTHTMAGDTMKLFSTRDRVVVMC"
     gene            complement(25516..25953)
                     /locus_tag="ACIE68_002303"
     CDS             complement(25516..25953)
                     /locus_tag="ACIE68_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908610.1"
                     /translation="MSSRKLFAAKSLFALAMAIAGAHFLHDLDHNAVISAAGALSTIS
                     GVLFGFVLASVTMLSSFDSSKGIVGALKSNGVLKGIIEGLFDTGTTLITACLLAMISM
                     FAPVVSNLPLDYYALLLAGAYLIISMITFFFNWKSLSKIISYI"
     gene            complement(25966..26922)
                     /locus_tag="ACIE68_002304"
     CDS             complement(25966..26922)
                     /locus_tag="ACIE68_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908611.1"
                     /translation="MTVAPKDQSLKSVRVGFYTSSENIRTSNKGLTAEAFKNLYDRCS
                     KDFKMTHYADFGERELKIHFLDISPEDGSYFGFMSRRRKSATLAYITDKEWVEEKIPL
                     AGAKTLSERTYFIYYPQTDILVLSLNHLGPRHSDLAFLLFNSSDRGVPVSFEAIWKEE
                     SIKELLETGSNLRSCEISVAIPRNFNAAQYQLDGMFANQMIEMIRGTSSSHLTISLRG
                     HSPLKRKAKGWLEDDVKKSLKEMLEKFPGGDGRLTFEKANVVAQGDKKKKSLVDEVLT
                     VKKMVPIQADGYPTDTDVKNAMLQAKNDNLRYLTQYYLVSQT"
     gene            27539..27820
                     /locus_tag="ACIE68_002305"
     CDS             27539..27820
                     /locus_tag="ACIE68_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFX5908612.1"
                     /translation="MIRDRKAEELESKGLYRRAAARWMEVMLLCTEDDDREWIKRRRE
                     TCLENVKRPPVKVEEFGDLHKAVTETQHRMGIAQPNGNAFRLNGGKRQR"
     gene            28117..28302
                     /locus_tag="ACIE68_002306"
     CDS             28117..28302
                     /locus_tag="ACIE68_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908613.1"
                     /translation="MTPREIALLTIAKLEHGGHQLTQADQREIERSVNADIARRDRFR
                     EMMRAPAYQWKKPAPRR"
BASE COUNT         7735 a         6512 c         7249 g         6852 t
ORIGIN      
        1 gacaagcgag tgaatcgcca ggagcttact aaagtaagtg actggggtga acgaacgcag
       61 tcgcagtaca tgcaacttga agtatgacga gtataaatgg aatggtacat gggcaaatat
      121 attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg gatcgagagt
      181 ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg cgtagtgggg
      241 atgccagatt tacatcccgg acgcggctat ccgattggcg cagcgttctt ctctgttggt
      301 cgtttttatc cggcaagacg tcgcggtaac ggtgctggaa acagaaacgg gccgctactc
      361 tgacacgctg cgttcggcgc tggtttctct ggatggcgat aacgcatggg cattaagcga
      421 aagctggtgc ggcactattc agtggatttg tccgagtccg tatcggtctc atcatgggcg
      481 caaaaactgg tttctgggca tgctaacaaa cggctggcgc gattgctgat tgcctggaag
      541 ctggagcagc agcaacagga aaatagcgcg gcgctgaaat cgcagcggcg aatgttccat
      601 caccagattg aacgtggcaa cccgcgacgg acatttacag ggatggcttt tatcgaagga
      661 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
      721 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
      781 ttgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
      841 atgtccattt ccggatacgg ttttttaaac agaccacatt ccaggcccgc gtggataatc
      901 tggatgttcg gcgtcttgtt gaacaggcgc tgataggttt cacgtaccag atgcatcacc
      961 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1021 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcagt
     1081 gaacggatca ggcagtgaat ttctacgtta ttgtcagtca tggtcaccac accgacgttc
     1141 agggaggttt cgaccacacc ttttgccacg tcggaattgc ggatcacacc gttcggggtg
     1201 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagagcagc tttatcgttc
     1261 gctacagagt ccagcaacaa ggccagattc ttctctttct ctgccagctc gtttttcagg
     1321 atgtcctgat aggtattcac cagagatttc agggcgtcga ctttatcagc tgcgacagca
     1381 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccaccgtt gaaatcgata
     1441 aggcgcaggt ccagctcttc cgcatgaccc gccaggaagc gcaccagcag tttgttggca
     1501 ttacccaggc caacgtggat ttcaccgccg gagtgaccgc cttttagacc ttttaaggtt
     1561 aacttgaagg tttcaaagcc agctggaacc gcttcacgat ccaaatgcag gttggaggtg
     1621 aagtcgatac cccccgcgca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     1681 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     1741 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     1801 accgccagcg cagacgccat accaatgccg ttatctgcac ccagcgtggt gccgcgcgct
     1861 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     1921 tcgttatttt tctgcggcac catatcgagg tgggcctgca agacgaccgg tttacgattt
     1981 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2041 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2101 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2161 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2221 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2281 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2341 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     2401 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     2461 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     2521 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     2581 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     2641 gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     2701 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     2761 ctttgtcacc atctttgcaa aaccggctgg tcgtccgctg gttgataatt atgttgttga
     2821 tatcccgcaa gatacctgga tcgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     2881 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     2941 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3001 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     3061 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     3121 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgtg
     3181 aaatcctcga cgtccaggca cgtattgtga tgagcgatgc tgaacgtacc gatgatgatt
     3241 tatacgatac cgtgattggc taccgtggtg gcaactggat ttatgagtgg gccacccagg
     3301 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgacact
     3361 ggctgcatgc ggctacgttg tacaacattg ccgcctatcc tcatctgaaa ggagatgacc
     3421 tggccgagca agcgcaggct ttatcaaacc gcgcctatga agaggccgcc cagcgtctgc
     3481 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     3541 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     3601 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcga
     3661 tgctgactat tgatatgccg tcggtgggct tttcttcaaa atggaagctc acccaggact
     3721 ccagcctgtt gcatcagcac gtcttaaagg cgctgcctaa cgtaccgtgg gtggatcaca
     3781 ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatacc
     3841 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
     3901 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt
     3961 tggggatgca tgatgcttcc gatgacgcgt tgcgcgtgga gctgaatcgc tattcattaa
     4021 aagtgcaagg attgctggga cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     4081 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
     4141 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
     4201 tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc taaattttgc caatttggta
     4261 aaacagttgc atcacaacag gagatagcaa tgacgttacc gagtggacac ccgaagagca
     4321 gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata
     4381 atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca ccggaagtgc
     4441 gtgaattctg gggctggtgg atggagctgg aagcgcagga atcccgtttt acctacagct
     4501 accagtttgg tctgttcgat aaagcaggcg actggaagag cgttccggta aaagacactg
     4561 aagtggttga acgactggag cacaccctgc gtgagtttca cgagaagctg cgtgaactgc
     4621 tgacgacgct gaatctgaag ctggaaccgg cggatgattt tcgtgacgag ccggtgaagt
     4681 taacggcgtg agtgaaatgt gccggatgca tcacatccgg caataatcat taaaactgat
     4741 aggtcatgcc aaccgcgaca atatcatcat tattaatatt caatttgtta tcgctatcca
     4801 gttggttgat tttataatca acaaacgctg acatattttt gttgaaataa tatgtagccc
     4861 cgacatcgat ataattgacc agatcttcat caccgatacc ttcaatatct ttccctttcg
     4921 ataagacata acccagcgat ggacgcagac caaagtcaaa ctggtattga gcgaccgctt
     4981 caaagttctg tgtcttattg gcaaagccgc cagttattgg cgtcattttg cgtgtttcag
     5041 aataaaaagt tgccagataa atattattgg catcgtattt cagacctgtt gcccatgctt
     5101 ctgcacgctt gccagtgcca cggctttgca ggttctgctc gttggtgcga tctgagttgg
     5161 tataggcacc actaatggcg aaatcgctgc cgccaaagtc atatgtcaat gacgtgccga
     5221 agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt tttcccttga tattgcaggg
     5281 ttaagttcag gccatcgata acgccgaaga agtcggtgtt ccgatacgtc gccagaccgc
     5341 tggcgcgttt ggtcataaag ttgtcggtct gcgccgagga gtcgccacca aattccggga
     5401 acatatcggt ccaggcttcc acgtcataca acgcgcccag gttacggcca tagtcgaaag
     5461 aacccaaatc tttatacttc aatccggcaa aagcgagacg cgttttttgc tgtgcagtat
     5521 cactctccgc tttatttccg gcaaactccg cttcccaacg gccatagcca gtcagttgat
     5581 cgttaatttg tgtttcgcct ttaaaaccaa aacggatata actctggtcg ccatctttac
     5641 tgtcgttatc actcatataa tgcatggctt taactttgcc atagacatcc agtttattac
     5701 cgtctttgtt atatatttct gcggcctgta cggatgcaga tgccacaatg cccatcacca
     5761 ctaatgccag agtgctcttt ttcattttca ttcctgattt taattaacgc gcgaatattc
     5821 agcgggagag ccccgttgaa aacaggaaag tttttaacct gagattgtta aagatatatt
     5881 acagattaat aatattctta aaatgtggta atttattaaa tctgtaataa aagcgtaaac
     5941 aactgccgct aggcttgctg atcccgcgca acaaaacgcc atgctttgct tgcagatggt
     6001 tggcaaccga cgacagtcct gctaaaacgt tcgtttgata tcatttttcc taaaattgaa
     6061 tggcagagaa tcatgagtga cagccagacg ctggtggtaa aactcggcac cagtgtgcta
     6121 acaggcggat cgcgccgtct gaaccgtgcc catatcgttg aacttgttcg ccagtgcgcg
     6181 cagttacatg ccgccgggca tcggattgtt attgtgacgt cgggcgcgat cgccgccgga
     6241 cgtgagcacc tggggtaccc ggaactgcca gcgactatcg cctcgaaaca actgctggcg
     6301 gcggtagggc agagtcgact gattcaactg tgggaacagc tgttttcgat ttatggcatt
     6361 cacgtcgggc agatgctgct gacccgtgct gatatggaag accgtgaacg cttcctgaac
     6421 gcccgcgaca ccctgcgtgc gttgctcgat aacaatatcg ttccggtaat caatgagaac
     6481 gatgctgtcg ctacggcaga gattaaggtc ggcgataacg ataacctttc tgcgctggcg
     6541 gcgattctgg cgggtgccga taaactgttg ctgctgaccg atcaaaaagg tttgtatacc
     6601 gctgatccgc gcagcaatcc gcaggcagaa ctgattaaag atgtttacgg cattgatgac
     6661 gcactgcgcg cgattgccgg tgacagcgtt tcaggcctcg gaactggcgg catgagtacc
     6721 aaattgcagg ccgctgacgt ggcttgccgt gcgggtatcg acaccattat tgccgcgggc
     6781 agcaagccgg gcgttattgg tgatgtgatg gaaggcattt ccgtcggtac gctgttccat
     6841 gcccaggcga ctccgcttga aaaccgtaaa cgctggattt tcggtgcgcc gccggcgggt
     6901 gaaatcacgg tagatgaagg ggcaactgcc gccattcttg aacgcggcag ctccctgttg
     6961 ccgaaaggca ttaaaagcgt gactggcaat ttctcgcgtg gtgaagtcat ccgcatttgc
     7021 aacctcgaag gtcgcgatat cgcccacggc gtcagtcgtt acaacagcga tgcattacgc
     7081 cgtattgccg ggcaccactc gcaagaaatt gatgcaatac tgggatatga atacggcccg
     7141 gttgccgttc accgtgatga catgattacc cgttaaggag caggctgatg ctggaacaaa
     7201 tgggcattgc cgcgaagcaa gcctcgtata aattagcgca actctccagc cgcgaaaaaa
     7261 atcgcgtgct ggaaaaaatc gccgatgaac tggaagcaca aagcgaaatc atcctcaacg
     7321 ctaacgccca ggatgttgct gacgcgcgtg ccaatggcct tggcgaagcg atgcttgacc
     7381 gtctggcact gacgcccgca cggctgaaag gcattgccga tgatgtgcgc caggtgtgta
     7441 acctcgccga tccggtgggg caggtaatcg atggcagcgt actggacagc ggcctgcgtc
     7501 ttgagcgtcg tcgcgtaccg ctgggggtta ttggcgtgat ttatgaagcg cgcccgaacg
     7561 tgacggttga tgtcgcttcg ctgtgcctga aaaccggtaa cgcagtgatt ctgcgtggcg
     7621 gtaaagaaac ctgtcgcact aacgcggcaa cggtggcggt gattcaggac gccctgaaat
     7681 cctgtggctt accggtgggt gccgtgcagg cgattgataa tcctgaccgt gcgctggtca
     7741 gtgaaatgct gcgtatggat aaatacatcg acatgctgat cccgcgtggt ggcgctggtt
     7801 tgcataaact gtgccgcgaa cagtcgacga tcccggtgat cacaggtggt ataggcgtat
     7861 gccatattta tgttgatgaa agtgtagaga ttgctgaagc attaaaagtg atcgtcaacg
     7921 cgaaaactca gcgtccgagc acatgtaata cggtagaaac gttgctggtg aataaaaaca
     7981 tcgccgatag cttcctgccc gcattaagca aacaaatgga ggaaagcggc gtggcattac
     8041 acgcagatgc agctgcgctg gcgcagttgc agacaggccc cgcgaaggtg gtggctgtta
     8101 aagcggaaga gtatgacgat gagtttctgt cattagattt gaacgtcaaa atcgtcagtg
     8161 atcttgacga tgccatcgcc catattcgtg aacacggcac acaacactcc gatgcgatcc
     8221 tgacccgcga tatgcgcaac gcccagcgtt ttgttaacga agtggattcg tccgctgttt
     8281 acgttaacgc ctctacgcgt tttaccgacg gcggccagtt tggactaggt gcggaagtgg
     8341 cggtaagcac acaaaaactc cacgcgcgtg gcccaatggg gctggaagca ctgaccactt
     8401 acaagtggat cggtattggt gattacacca ttcgtgcgta aataaaaccg ggtgatgcaa
     8461 aagtagccat ttgattcaca aggccattga cgcatcgccc ggttagtttt aaccttgtcc
     8521 accgtgattc acgttcgtga acatgtcctt tcagggccga tatagctcag ttggtagagc
     8581 agcgcattcg taatgcgaag gtcgtaggtt cgactcctat tatcggcacc attcaaacat
     8641 ctccccaagt ctactaaagt ctttcaaaac cccttataat ccgcgtatta aagccccgtt
     8701 cgtcttttga cgtctactaa agtccctcaa aatctacggt cagatggggg tacttatggg
     8761 ggtatttgct gttcggttta gtggaggtac ccccaagtga aactcaatgc ccgtcaaata
     8821 gacacagcca agccaaaaga gaaggcttac aagctggccg atggtggcgg tttgtatctc
     8881 ttggtaaaac ctaatggagg taaatactgg cgacttaaat atcgtgtagc tggtaaagag
     8941 aagctattgg cactaggtgt gtatcctgag gttactctag ccgatgctcg ggcaaaacgt
     9001 gaagatgcga aaagaggtat cgctggtggt atcgatccga tggaagcgaa acgagaggaa
     9061 aagattgccc gggaaacgca gttaaacaac accttcaaag atattgccct tgagtggcac
     9121 agcagcaaat taaaaaaatg gtctgctggt tatgcttcag acatcctcga agccttcaac
     9181 aaagatgtgt tcccttacat tggcaaaaaa ccaatcgccg aaatcaaacc acttgaactt
     9241 ctgaatgtgc tgcggcgcat cgaggggcgc ggtgctacag aaaaagccaa aaaagtgagg
     9301 cagcggtgcg gggaagtttt ccgctatgca atagtcactg gtcgcgcaga gtataaccct
     9361 gcaccagacc ttactagcgc gatgcaaggc catgaatcta atcattaccc tttccttaca
     9421 gccaaagaat tacctgattt tttcaaggca ttgtccagtt actcaggaag cgcattggtt
     9481 gttatggcgg ctcgtctact gattatcacc ggcttgcgaa ctggcgaact acgtggcgca
     9541 ttatgggatg aaattgattt caacaaggct atatgggaga tacccgcttc acgtatgaaa
     9601 atgcggcggc ctcatattgt gccattgtct gagcaggctc tttcgcttat tgggaagatt
     9661 agagaaataa caggcaatta ccctcttatg tttcccgggc gcaatgatcc aaggaaaaca
     9721 atgagtgagg ccagcataaa ccaagtgttt aagcgcattg gctacgctgg acgtgtaact
     9781 ggtcatgggt tccgacacac tatgagcacg attttgcatg agcagggcta taacaccgcg
     9841 tggatagaaa cgcagctcgc tcacgttgat aagaactcaa ttcgtggcac atacaaccat
     9901 gcgcaatatc tggatggaag gcgggagatg cttcaatggt atgccgacta tatggattct
     9961 ctcgagcatg gcggaaatgt ggtgcatatg gtgtttgaaa aacacgcatg aaacaactgg
    10021 acaagtatac agaggtttac taaagtatag gggagtagat tgataaaaac tcacagagga
    10081 ttgctattta acagttagcc ctggagaagg gcaatcgctc ttttacaaac gaaggtgtat
    10141 tgagccttct acgtcgctga caaagcggaa tgttctttaa caggctttgg cgctgacaaa
    10201 gcgcaattgt tctttaaaac tactggatgt cacgcctaat agcgtgatcc tgccgaaatg
    10261 cagggtagct ctacccttgt ggagcaaaca aaccaaaaca atctgactat cttttataaa
    10321 agaccgcaat atgtttttca tattgtggtc tttggcgtat tcgacatttg acgcaaataa
    10381 aactctcgcc attcgttgca ggagggggtt attgcgtcat tagtacagga tattaataaa
    10441 tggctaatta caattcatta attcgtctct ctgaagttca aaggcgaact ggttatagca
    10501 aagcatggat ttatcgtctt attagtcagg ggaggttccc taaacaggta aaaattggaa
    10561 gtcgggcaat tgcttttgtt gagtctgaaa tagatgaatg gattgagaag tgtatattag
    10621 aatctagaga cgaggtggcc tgatggaaaa gaaaaaccgc ccattacagg cggctaattc
    10681 agatattcgt gtatctgatg ttacgcccct tacaaaatcc cctcaagcac caaagcgcac
    10741 accgaaaaag catcgtgcca gagtctatat gctgcgtact ggtatagagg gatggacaga
    10801 aaatgacatt cttcgctact gccgtctgtc ttctggtcgt aactatgcaa cagagttaga
    10861 acgccagctt ggaatcactc tggagcgtat cgacgaaaag aatcctgatg gtatcggaac
    10921 acaccttcgc taccgtttct cctgccgtgg tgatgttctg aaagtgatca ctcatattaa
    10981 ccatcttgcg aacataaatg atcacaacgg actttctcag caggaaattg ccgacattct
    11041 gaaactctac ccggacgcgt ttaacgccgc ctaacggaga ctgaaaatga acatcgaaaa
    11101 aagcagatta atttctgagg ccgcccctca tctgaacgcc tctctgggca caattaacgg
    11161 taatgaattt gccgcaattg tcccggttat tcctggtcat atcggtgggc gtgaaaccaa
    11221 tattgttagc gcaaaagcgt tacacaaagc gttgggcgtg ggaaaagact tctctacatg
    11281 gatcactgat cgcatctctg aatatgactt caccattggg cacgattact cagtccataa
    11341 aacaatttcc ccaaatttgg ggaaaagccc gaatggcgcg gcttacagca agataaagca
    11401 ctctggcaga cccggcaaag actatctgtt aagtgtcgga atggcgaaag aactggcaat
    11461 gatcgaacgc aatgatcagg gtcgcgctat ccgccgttat ttcatccagt gcgaggaaga
    11521 attacagcgt agcgtgcctg aaatcgccgc ccgctatcgt cgccagctaa aagcccgtat
    11581 tagtgccgca aacaacttta agccaatgtg cgatgcgctg aatatggccc gtgccgagat
    11641 ggggaaaacg acgcagcaac accactacac aaacgagagc aatatgattt ctcgtatcgt
    11701 tcttggtggg ctaactgcta agcagtgggc gcggataaat ggctattctg gcgaacctcg
    11761 cgaccatatg aacgcagaac agcttgagca cctctcatat ctcgaaagca ccaatatcac
    11821 gttaattgat atgggcatgg aatatgagca gcgcaaagga gaactcaccc gcctgtcgca
    11881 acgctggctc gccaagcgtc tggaggcgct caatgtctaa gccaacagga ataccacaac
    11941 cacaaaaacg ctacaaagat gcccacggag cactcgttac tgtcgaaagc gtgtctcaca
    12001 accgagtgac gttttatcgc gacgggtatc aatcgccatg cgtacaaccg ctggcgcgtt
    12061 tcatgaagga gttcgcggag gttaaccaat gatacccgtc cagaagaaga cattttcact
    12121 ggctggtatg tcgccaaaat ccagcaatat gacggcaaag tcaggcatta atacagcaga
    12181 tacaagcaaa gtttatcatt tgctggtggt cggaacggat gccttaacca taccagaaat
    12241 ctcgggcgat gatattagca ttgagaaggt cagcggctgc gccagagaat ttctggtcgt
    12301 agatgatgtc ttccgctcgt gtagtgactc cacaaaaaca ttcgtccacg cgcgtgacgt
    12361 taacgaaatg agcgcgatgt actgtgcatc tggtgcttcc aatagcgagt tttcggagtc
    12421 cataaaaaag agcttgccgt tatgcggcaa cacggtttat ggttataagg cacctcataa
    12481 aacgggtgcc gggattggcg tcctgaaaat tctgaaggcg acatatgacg cgccgagcgt
    12541 ctttttttat gtcgtaggtc tgactcaccc attttttggg cgttggtgta taatccagcg
    12601 cctttgtcaa tcaatggtgg ctcaggcggg ggcttcttcg gaagcgccgg tatccttcag
    12661 agccggttac gccaaccctg tctgggctac caccagcgaa attggcgttt ccggtggtag
    12721 cgtaacctgc tatctgaagg aggctgccac atgctggcta ctacccctac ccaaaaaccg
    12781 caatttatct ggattatcgc cgcagttcgc cgcgattgcc cgataattac cgccaaaatt
    12841 catcatattg ctgccgagtc tgaacgcgat gctcgccgtt ctctggtgcg cgatcacgtc
    12901 tgcttttttg ctggtcgtat ccgcatggag gtggcacatg attaaaacct acgatgtgca
    12961 tatggatccc ctcgaacgca caagccagat catcacactg acagaagtga ttaatgacat
    13021 tctggtgagc aactctccct cacgagacga aaaactgaag gcgttactcg cgatattgga
    13081 tctcgccgtt cgtgacgttc atttcctgct ggaaggtgga caaatgccat gcaaaacggg
    13141 ggcaaccaat gaataactca attaataccc ctcgccttac gtccgcactt caattaatcg
    13201 agcaagcagc ggctgtcctg cttgctgtca gtctttcggc tgaagagatg gacgctgctg
    13261 atgtcgtgga tgcgattaaa gcgtgctcat ctttggttaa cgatgcccgt gcagagctgg
    13321 taattcttgg gggtgaaaaa tgaatatcaa cttaatttat cgtcatccgt gtgagctgga
    13381 aattgaatca ttgctggggc gcgaagagcc atatccagac acattcactc ccgcagattg
    13441 cgcgactgaa cggcttacca gagcgcgcac aggtctggtt catgtgatga atgagattat
    13501 tccctcggtg ggaggggaac aggcgacagt aatcaatagc tggctacaaa aagtcacctc
    13561 cctgatagat atcagtttaa tcgatgtgga gagtgcgaaa tgaccaacat ccagctcatt
    13621 gaagcgcaat gtcgcatcga acaggttcag actgttttag ggttctggct tgaaggggcc
    13681 agcccaagca acagagacaa gttaatgatt ggcgcggtta tgtcactgct caatggcgta
    13741 ccagaagcta ttcaggaagc agacgaattg ttgggcaaat atgagttaca gaatcattca
    13801 ggcgaggcga aacatgaata atttcttaac tttccatgca gaagcaacgc ctgacggcgt
    13861 aaacatcatg caccgcagca acgatggcat gacagaacgc gttgagaccg tctcatatat
    13921 tgatgccgta aatcgtctgg atgccgggga ttatgacgat aaaccagatg aaggtatgtc
    13981 aatacatctc gctatagccg atggcggcaa ccagggatat ttcgattaca catcacagca
    14041 taacgtcatt atgtggagat ggctgatcgc aacagtattc atgctggaaa tgagagagga
    14101 aaacggcact gtcagcatca ttgatgatac tggcaatcct tccgaagtgg ctgtttattc
    14161 caacggcatc gttgccatgc cgctgtatcc agtagcagag cgcctcgcta tggcaaacaa
    14221 cattgaggga gcgatgatag aacgttttgg catcgagtct ggcacagaac gctccatcat
    14281 tttctacaga gccatgatgg atgtggaaca aggagcactg actccatttg gtcgtgaaac
    14341 gcttgctgaa cttcacaaca gctttatagc tgaactgaat gaaaacggca tgccatcgga
    14401 accagtgacg cactgaggat ggaaccatga ttactaaaaa tttccgactg aatgcgctgg
    14461 caaataagta tgcgtcggca ctgtataacc acattacctc caccagcgga ggggactatt
    14521 tcatggttga tgcggacggc gaggccgtac gtgtggaaat cgtcaatgga gtaaaaggcg
    14581 ttcgcagcct gatcgatagc tacacactgg cagccatgaa agtgttctac ccgcagtggg
    14641 aaaccgtggg gatcgagctt attgagcgtt gcgtcactaa ggacggattg actgacgtcg
    14701 ggcgtgagat atggcaaagc atggttaatg acatgggcgc aactgtagcg ggaggttctc
    14761 atgcgtgaaa atgcagaaat ggcgcttagc tccgcaatcg gagagcaggt ggcaaagatt
    14821 gctggtgctg tgtggattca taaccttcat tctactggtg aagagaaaat ggcaattcag
    14881 acgccagagg gacgcaccat tactacatct ctgaagccgt cagatgtttg tgatctgatt
    14941 tgcgcattca tgtacccagc aatgaggact gtccacggcg ataagtggaa acttgcgaca
    15001 accgctgaat ttgatatgtg gctaaacaat gatggcatgc tgactgatta cggaataacc
    15061 aagtggcaga tgctggttag ccatatcgca aacgctatag accacgtggg gtacggtgat
    15121 gcgaaacatt gatcttatcc gtcaggttat cagtgcgtct gaaaacaact ggcctcatgt
    15181 gctgggctgc ctgaacataa atgtccctga ctctccgcgc cgtcatgctc cctgccctgc
    15241 atgtgggggc aaagatcgat tccggttcga tgacaacggg cgcggtagct tcatctgtaa
    15301 tcagtgcggc gctggtgatg ggctggattt aattaaacgc gtaaataact gcgacacaac
    15361 agaggcggcg cttcttgccg ctgatgttct gggtattgat taccggacaa cggaaacacc
    15421 agaagccacc agccagaaac gggaacaact ggaaaccgag cgccagcgac gcgaacagga
    15481 gcgcctgaaa agggcagaga aggacgaaca acaaagacgg gatacgtttt cccgtcagtt
    15541 tgatgacatg cgcagaaagg ctgtaaacgg caaatctgat tatctggttg cgaaaggggt
    15601 aggtgatttt acattccccg tgttgcccga tggatctctg ttgctggcgc tggtggataa
    15661 atccggcgca gtaacagcag cacagactat tacctcacat ggtgaaaaaa gactcctgac
    15721 aggttcagca aagcgagggg catatcacgc cataaacgca ccagaaacaa cacagagcat
    15781 cttaatcgca gaagggctgg caacggcatt atctgcccat ttaatgcgtc cagaggcgct
    15841 gacagtggcg gcgattgatg ccgggaacct gttatatgtc gcccaggtac tgcgggataa
    15901 atttccttca gcacaaatca tcattgctgc ggataacgac cacagcgaag gaagacaaaa
    15961 cacggggaga atagcagcag aaaaagcagc tttatctgtt tctggatggg tggcattacc
    16021 cccgacagat cacaaagcag actggaatga ttaccaccaa aaacacggca ttaaatgcgc
    16081 cacagaagcg ttcaataagt caatgtacca accacagggt aatggcgtga aacaggagcc
    16141 acagaccatt gagggaagcg attttaaggt tatggatacc gatccactga aaccaagaat
    16201 agaaagccgc gaggatggta tttattgggt atctccgaga gctgacagcc agagtggaga
    16261 aatcatcaat aacgaaagct ggttatgttc tccgttaagc gttatcggta ctggcagaga
    16321 cgataaagac cagtatctca ttcttcgctg gctgtcgttt ggttctgaaa cgccgacaac
    16381 cgctgccatt ccactggctg atattggtga acgtgaagga tggcgcacac ttaaagctgg
    16441 tggggtaaat gtcacgacca aaagcagcct cagagcaata ctggcagact ggctacaacg
    16501 cagtggctct cgtgaattgt ggcgtgttgc tcatgccact ggctggcaat gcggagcgta
    16561 catcatgcct gatggggaaa tcatcggaac acctgaaaat cctgtgctgt tcagcgggag
    16621 aagctcagcc gcagccggat ataccgtctc agggagcgcg aaaagctggc gtgataacgt
    16681 ggcgcgtctg gcctttggta actattcaat gatgaccggg atcggcgcag cactggcagc
    16741 gccattgatt gggctggtgg gcgctgacgg gttcggcatt catttctatg agcagtcgag
    16801 cgcaggtaag accaccacgg caaacgtggc gagcagctta tacggcaacc ctgatttact
    16861 gcggctgacg tggtacggaa ccgcgctggg gctggcaaat gaggccgccg cccacaatga
    16921 cggactaatg ccgcttgatg aagttggtca gggcgctgat cctgtcagcg tttcacagtc
    16981 tgcctatgcg ttatttaacg gtgtagggaa actgcaaggc gctaaagatg gtggaaaccg
    17041 ggatctaaaa cgctggcgta cagtggcaat cagcaccgga gaaatggatt tagaaacatt
    17101 tatcgctact tccgggcgaa aaaccaaagc cgggcaactg gtgcgactgc tgaatattcc
    17161 gttgagtaaa gcagtacgct ttcacgatta ccagaacgga aaacagcacg cagacgcact
    17221 gaaagacgct taccagcatc accacggcgc gtccgggcgt gagtggatta aatggctggc
    17281 agaccaccag caacaggcta ttaaaaccgt tcgtgattgc gaaagcagat ggagaagcct
    17341 gatcccatcc gattatggtg agcaggttca ccgtgtagcc gcgcgatttg ccattctgga
    17401 ggccgcattg ttgttaggcg aggttgtaac aggctgggat gctcagacat gccgtgatgc
    17461 gatacagcac agctataacg catggttgcg cgagttcggc acaggtaaca aagagcatca
    17521 gcagattatt gaacaaacgg aggcatttct taatgcctat gggcttagcc gttttgcacc
    17581 atttccatac agcccggcag atttgcccat caaagattta gccggatacc gtcagcgtgg
    17641 agaacatgac gaaagcccaa tgattttcta cacattcccg gccacatttg aaaaggagat
    17701 cgcatgtggt ttcaacgcaa aacagtttgc tgaagtgttg aaaaaagccg ggatgctaac
    17761 cccaccaaac agcggtcgtg gataccaaag aaaatcacca cgtattcagg gacgacaaat
    17821 aaacgtttat gtactcaatt accagccagg agattataac tcatctgaag aatgagcttc
    17881 ttcatgtgcg tagaaaaggt gttggttcag ttggttcagt tggttcaatt tgtaaagagt
    17941 attgttttat aaggatttta ttctttttat tgaaccaaca ctgaaccaac aacacggtat
    18001 tttgaaccaa cacaatgtga ctgaattgat gcgcacaaca cctggctggc atcagagagt
    18061 gttttacgag actttcgtaa aatcctgtgt acaagggcgt gacgtttttg aaagtgagca
    18121 ctacgaaatt ttcgtagttc aaaaaacagc ctctgagaag ttgttatgcc tgttcagcaa
    18181 accatccgca tagctgatcg cgaatcaaat taccaatgga gccagaagca agggcagaac
    18241 tgaaaagcgc ctgccgtgaa gagtaatgcg acaccataaa tatagttttt agagtccata
    18301 aaaaacctgt caaaacctga cacacagaac gatgccacca gctactgagc ggtggcattt
    18361 ttttgtgcta tttgtttcat attttgcaat tatgatgatg aatattgcga tttgtgaaac
    18421 tataataact gttgttttat acagttctaa gggggcgtta tggctatttc agtaaagccg
    18481 gtattgataa gtgagaagca aatggaagcg ataaagaaaa ttcaggaaga gcagcgtaaa
    18541 aaatcaggaa ttggagtagc gccaactctg catgaaattg cacgagggtt aatagataag
    18601 gcgctggcag gctgtatgtg aaaatggatg gcatcgcatg gaactaaaat ttattgataa
    18661 ccctgtgcga ttacaggcct ttcttaacga gccaggcaat accgaaaata ttgtagagcc
    18721 ggggcacacg tactacatca agcctgatgc ggtatatctc ggcatctacg aaggattagt
    18781 gctggctggc gttcatgaag tgcgtaactt ctggcatagc gttgttgaat gccatgcggt
    18841 gtacgacccc ggattccgtg gtgaatatgc actgcaagga catcgattat tctgcaaatg
    18901 gcttctcgaa aactcaccat tccttaacag catcactatg gttcctgaca ccacgaaata
    18961 cggacgggca attatccgtt tgcttggcgc tacccgtgtt ggtcaccttg atgatgctta
    19021 tatcagatgt ggtaaacccg tcggcgtaac gctttatcag ttaactcggc aacaatataa
    19081 ggaattcagc gaatgttaat tacccatata gctcataaac atctcaatcg tgccgtatac
    19141 gaaaaaggcg gtgatggtgg tgcctctaaa gctcaggcga aggcccagca gcaggcaatt
    19201 gatttgcagc gcgagcagtg gaatacggtg atgaataatc tcaagccgta tgcagaagtc
    19261 ggattgcctg ctttacaaca actgcaagga ctgatgactc tggaagggca gaataaagcc
    19321 gcgaatgatt tctttggttc tggtttatat aaaacacagg cagatcaggc ccgttatcag
    19381 aaccttgtct cagcagaagc taccgggggg cttggctcaa cagctaccag taaccagtta
    19441 tcttccattg cgcccatgct ttataacaat tggcttagtg ggcaaatgca aaattatgga
    19501 aacctgctaa atgtcgggat gaatgcggca tcagggcagg caacagcagg gcaaaactac
    19561 gccaataata ccgggcaact cttgcagggg cttggcgcta ttcgtgcagg tcaggcgcag
    19621 cagccatcca gtcttgcgcg tggtattggt ggggcggcat caggcgcttt agcgggtgcg
    19681 caacttggtt ccgttgttcc gggtataggt aatgtcgccg gggctattgg tggtgggctt
    19741 attggtcttg ttggggggct tggattctaa tggcgacatg gcaacttggc gggctgccta
    19801 gcatggcacc gcaaaatgat aatgctcctc gttcttccgt tcctcagcct gtgcagtatc
    19861 agcagcagcc aaacgtaggt ttaatggcgc tacaggggct aagaggcgtt gctgagatta
    19921 atcagcaggc gcaaatttcc cagcagctaa aagaatatca ggcacggttg ggtagtgcgg
    19981 tagctaataa tgatcgtgtt ggcgtgcaga agttaatggc ccagtatccc cagtttatgg
    20041 ctgattctca aaaacagatg ggattcattg atgctgagca aaatcggcag accgctgacg
    20101 ctgccatgaa tctgcgtctt gcgtcacaaa ctggagatcc actgaccatg cagaaggcca
    20161 ttcagcaggc aaatccagta ttacagcgat ttggtcttcc tgctgaagag gtttatcagt
    20221 cttggcagaa tgacccacag gaattcaaca gaatcactga cctcatccac ctccatgctg
    20281 atccaaatag ttactttagt gttcaagaga agcagcaagg gcagagtatt gcacagcaga
    20341 acgcagatac atctgcatat aatgctcagg ttaatgcgga gcagggtaat cggcgtctcg
    20401 atatggagca gttcaaagtc atatccgata accaactacg cacgagagaa atggttctga
    20461 aagcacgact tggacgtgct caaaatgaca tcgagagaga caagctcaat gctgaactga
    20521 gcaaggtaca gcaggcgaaa gtggagaaga tgaacgataa aattaatact gttgcaggta
    20581 atatcgactc cttgaactct gcgcttgaag tgggtcatga actggcggga attgtaggga
    20641 aacatccttc ggccgttacc cgttcgcaga actataaccc cctgacgctg gggtttggat
    20701 tcctccctga tgtagcagaa gacacacgaa gcctacgcgc caaagctgat gaatacaacc
    20761 taaaggttat tctgcctagc ctaaaaggta cttttggctc taacccaacc gaaggggaaa
    20821 gggctgccct tatgcaatct caaaatggca ttaagtcagc aaccagcaat gaggcatttt
    20881 tgcgagagtt gaataaagcg caggacgtaa ttattcgaat gcagaaacga cagattgccg
    20941 ggctcggtat tcctgtaacc aaatctaagg acgaagaaac tgataccaaa atgctgttgc
    21001 aggatccttc actggttaag gattatgtga ccgcacatgg ctatctgccg gaaagttact
    21061 atcaggcaaa acttaaggga tggcaatgat ggcacaactg acagaaaaac agaaagccta
    21121 ttcccgtcat ctcgatgaaa aatatggttt cccatcaggg acgatggaaa cattagttgg
    21181 caatgaatct agtggtaacg gaagtgccga gtcaccgaaa ggagctagag gatttgctca
    21241 actgatgccc tcagtaatga gtgatgctgg ttatcgtgga acagatccgc gctcactgcc
    21301 atttgaaaag caaatggata tcgcagcaaa ccatctcaat aaaggaatga agcgatttgg
    21361 tgatataggt atggcattgg ctgggtataa ttctggctat gctcgtatta atgccgtaag
    21421 aaacggtaaa tctacactgc cagatgaaac ggctaattac gtaagtaaat ttgcagatgc
    21481 cggaataatc cctgatgaca gtgaaatctt gcagtttgca caaaccagtc aacatcgcaa
    21541 tcttcagcag cctgactact cagagcttga taaagaaatc tctcaggtgg gagatatttc
    21601 tgcatgggaa gcaaaacgtc agcaatttaa gaacatagaa gcaggactac caacagccag
    21661 ttcatgggag cagaaaagag ctcagtttct cgccgatgaa aatgccgggg aaccgcagca
    21721 gcaagtagcc aagtctaacc ttaatcctga gatggaatat ctttcgccag gactgaaagc
    21781 tgcgccagag cagatggcca gagggattgc taatataccg tttgatgtaa tggacgcagg
    21841 cgtgggggta gtcaatgcgg cgcaatcagc cggagcatgg gcagctcaga agcttggtgt
    21901 gggtgatggc acatacacgc ccatatcacc tgtctttcgc ccagtagacc aacctacaga
    21961 tccatacgcg caggctggtg agcagctagc accttacctt attacaggat taggtgcagg
    22021 aaaggcagct accgcagctt cttcagttgc caacgctcca cgtgctgaac gtgcggccac
    22081 aaaggtggct agtatgttgg cagaaaacct gccaggtaca ttggtgaacg cccaaaagaa
    22141 cgggcagatc agcccagaag acctagcaaa agagacaggt attggcctag caggatcgct
    22201 aggagcacgc gctttaataa agagcggaca gacagccagt gacgtaatgc gagatgtatt
    22261 gcggagtaat acaaatcaat caggccatgt gttaggggca gcaaatactc tggaaaccgc
    22321 caatgatgta tcgaaagcag cgcgttctgc cgtaggccga gagcatatcg catcgcaggc
    22381 atcaaacgtc agagatgatg tcgctaaggc ggcgaaaatc actggtatta atatcaacac
    22441 cctgacgcct ggaatgcgct caggtagtcg tggaattgcc caagcagaag gtgttctggc
    22501 atcaactcct gggaatgttc aggatgctca ccttacggcc ttcaatgaaa tatcctcaaa
    22561 gttgaataag cagttgtctg atttcggtgc tgagtctgga agcgcatcag aaaaaagcgc
    22621 agcaattaaa gagcgcatca tcgcgagcct cagtgatatg aaaacagcag agaatatggc
    22681 atgggcagaa ctgcgaagca cgatgccacg cgaaaggatg aaaatgagta atgggatggc
    22741 tgtcgtccaa gcagaaaaag cagccaatgt tccactcagc ccggagatga aacagtttca
    22801 ggctgccaac aaccaaggtg gcggcataac cttcgacggg atgaaggcat ggcgtgctaa
    22861 gtttgctgat gctgaacaag caaacacccg ggcaggtaag gccaatgccg cccggattgc
    22921 cggagaaatg cgcagagcta tcactgatga catgcgcatc atggctgaaa aaggaaactt
    22981 cctcacagaa tggcagaaag ctaacgatct ctctaaaagt tacattatcg ccaaacaaaa
    23041 tgccgaatct gtttttggtc gagatctcgc atcagacgct atggtaagaa aaggtgccga
    23101 tacgcttaag gcatccgcaa acactggaac tggagctttt caccgtctta tctccgcgct
    23161 gccggaagca gaacgccaac cagcaatagc atctattctt cagcatgctg tggcgcaggg
    23221 agagaaggga ggaatcggtg aagagtctgg tatccagtat tttgccaagc tcatgaccca
    23281 gcagaatgta aacgccatca gtcgtcatgc gccagatctc ggtagaatga tgaaagagtt
    23341 cggtgtactc gctaaagcca gcaccagacc gcagcgatac gtagagcgca cgggaagaac
    23401 aactgaggct ctcaaaacgc tcgattcagg ccttccaaag atcgtcggcg tgattatgaa
    23461 cggagttcaa cagtcaggtg cgatcgctgg aggctttggc ggtggtgtaa ttggttcgat
    23521 tagtggtttg gttgctggtg gcgcgctgag aaatactgtg gctaagattg gggcgacaag
    23581 aagtggtagg tatgcgatag agaaagccgt tcaggaagct acaaaagcag tgcgcgcagg
    23641 ggcatcacca gcagcgatcg ccgcagcaga gaaacgattt tcggccaata aaattgccat
    23701 gaaagcaatt cgtgatgccg ttggggctga agaattcgtg aaactgacaa gggccgggat
    23761 tgtagctacc ctcagtggca tgaaagatgt tagcgaatgg gaatagacgc tctaactaac
    23821 aaaaccataa tagccaagga cggcatactg atattgagcc aactatcaaa attccatatc
    23881 acattacggg cctgtggctt acattcgctt acagtgatga taatttataa ccacgcaatt
    23941 cttaggtgag attatcatga ttaaaaatct tcttttaata ttatccctgt cattattggc
    24001 tggatgcgta caaaaaccgt tagtaacggt caatgcttta tcttccgggc aatctgtcgg
    24061 taagaagttc ttgatcatcc ccgcaagcca agaactttgg aacaccaaac agattgaata
    24121 cactcaaata gctacggtag tagccgccag tttatcaagc caaggatata cacaagttaa
    24181 agatgaaaaa ttggctgacc aggtaattct cttgaacttt ttgagaagcg gggccatttc
    24241 aaatactcgt aatgtaacca tcccaatata cgggcaaact ggtgtgagct cagcaacaac
    24301 gtacggaaac tcgaacacca cgttcaatag ttatggtggt ggctacggta cagctaatac
    24361 cactggaacg gcaacaacag tttatacgcc tacttatgga attactggcg cgtataacac
    24421 aactgtgacg gacacgttct acggcgcagg ggtgatactc cagtcgtttg atgccaaggc
    24481 ttatctcaat aataggcaga ttaatatgtt atggaaagtt ggtagctcag tcgtctcgca
    24541 acagtctgat ggaattgctg attacaaggg gttggcgaga atagccgcta gctatgccgg
    24601 gaaaaataca gaaggtgata taacttttaa tgttgaccct aaagcaccaa attaactaat
    24661 aagcccggta cgccgggcat ttatgcccct attattcatg aatcgctttt tctagaaggt
    24721 atgcgacaaa cgttaagtga cattgcctac acattcgggg cgggatttca aatccgctcc
    24781 cagttgatgg ggggcgttac agtacgcgca tatccgcttt atgtgaaaca ggaagatttt
    24841 tctgatatca ctgccacagc aaaacaccaa tttcgtgacc agtacgcgcg cgaggggtat
    24901 cagttgcaaa aaattttgca actacgtccg accagtacgc acacgatggc gggggatacc
    24961 atgaaactgt tctctacacg agacagagtg gtggtgatgt gttgattatc ggcgtatatg
    25021 cgcacaggac gcgctatgac aatgttttat gcgggtacac caatcacatg tatctggtgt
    25081 gataaatcgc gttacatgac ctctcatgtt gtgctggtgg ttatcacgac gttctgaatc
    25141 tggcttttgt ctgatatttt gcgacaagta cgcgcgcgta acatcgaaat tatcggatgg
    25201 cgtatgttgc tggcaagata aagtcggcgt aatacaaagg cgttacttga tagcagcaag
    25261 attacaacaa tggttgttga atttgagcga tgtaaggaat tgatttaggg aggcgctgat
    25321 ggctaaaaag ttccactacc agcggatgaa tgcgccagcg atggccgttt ggtagtaaaa
    25381 gaggagccat tcctcaatca gttgaataca ttatgccaac taaatgggtg ggggcactgt
    25441 gtgggtattc agcggtgacg gtatttttta agccaaggac tatccgtagc agggggactc
    25501 attttgaaat atttatcata tgtaacttat tattttagat aaagatttcc agttaaagaa
    25561 aaaagttatc attgatataa tcaaatatgc ccctgcgagt agcagggcat aataatccag
    25621 aggcagattg ctgacaacag gggcaaacat cgagatcatt gctaataggc aggctgtgat
    25681 cagcgtagtt cctgtatcaa aaagcccctc aataatgcct tttagtactc cgttactttt
    25741 tagagcgcct acaattccct ttgaactatc aaagctactt agcatggtga ctgatgccaa
    25801 aacaaaacca aagagaactc cagaaatagt tgaaagagca ccagcagccg aaatgacagc
    25861 attgtgatct aggtcgtgta aaaaatgggc accagcaata gccatagcta gagcaaacaa
    25921 tgacttggca gcaaacaatt tccgagaact cataatgtat tcctgttacg tttgactaac
    25981 caaatagtac tgggttagat acctaaggtt gtcattcttt gcttgtagca tagcattttt
    26041 aacgtcagta tcggtcggat acccatctgc ttggataggc accatcttct ttaccgtaag
    26101 aacttcgtct acaagacttt tcttcttttt atcgccctga gcaacaacat ttgctttctc
    26161 aaaagttaaa cgaccatcac caccagggaa cttttctagc atctccttaa ggcttttttt
    26221 aacgtcatcc tcaagccatc cctttgcttt tctttttaat ggagaatgtc cgcgtaacga
    26281 aatagtcagg tggcttgaac tggtgcctct gatcatctca atcatctgat tcgcgaacat
    26341 tccatcgagt tgatactgtg cggcattgaa gtttcttggg atggccacag aaatctcgca
    26401 gcttcttaga ttggatccag tttcgagtag ttctttgatg ctttcttctt tccaaatggc
    26461 ttcaaaagaa acagggactc cgcgatcact ggagttgaaa agaaggaagg ctaagtcact
    26521 atggcgtgga cctaaatgat ttaaagacaa tactagaatg tctgtctgag gataataaat
    26581 aaagtatgta cgttctgaaa gagtctttgc cccggctaga gggattttct cttccaccca
    26641 ctctttatct gtaatatatg ccagtgttgc gctttttctt cttcgagaca tgaatccgaa
    26701 gtaggagcca tcctcgggtg agatgtctag aaaatgaatt ttaagttctc tttcaccaaa
    26761 gtctgcatag tgggtcatct tgaagtcttt agaacaccga tcatacagat ttttaaaggc
    26821 ctctgcggtt aaccccttgt tactagtgcg aatattctca gagcttgtgt aaaagcccac
    26881 ccttacagat ttcaaacttt gatcttttgg tgccacagtc atttgagctc cattgtactc
    26941 tgcgatagtt tttgcacacc ttgaaatcaa aatctccatg tcagtcacga gaaaataact
    27001 atggatgaaa cccatcaaag gcattgaata caggtggctt ttgagaatca tcctaacctg
    27061 gtgacggtgg ttcgcatatc ctgatggatg atcagtgatt aatcaatgcg ccaagcagat
    27121 gtgtctatgt gtttgttaat cattgcatga cagtagatcg acatgggaga tcacgttata
    27181 tagttgaatt gttaggatat ccatgcaccg atcaactgac taggctgatg tgctttttag
    27241 caggtgccat atagtagtta ggcggttctg tgaaatgatt actgttccag cataactgcg
    27301 gcataaagcg gtgctatata agctatttgc tggcggattt agtcttcacc gttaatatat
    27361 gtagtgacta tttgacagag tgttagttca cggacacttt ttgttggttc aaaatgagtt
    27421 gatgttggtt caatatttta gaaaaaacac aataaaaaca acgctcttta caaattgaac
    27481 caactgaacc aactgaacca acaccttttt gcttatctat ataatctgag aggcagaaat
    27541 gataagggac aggaaagctg aagagctgga gtcaaaaggg ctataccgga gagctgccgc
    27601 acgatggatg gaagtcatgc tgttatgcac cgaggacgat gatcgggaat ggataaagcg
    27661 ccgccgtgaa acgtgtctgg agaacgtgaa gcgcccgccc gtgaaggttg aggaatttgg
    27721 cgacctgcat aaagctgtta ccgaaacgca acaccgcatg gggatagcgc aaccgaacgg
    27781 taacgccttc cggttaaatg gcggcaagag gcaaaggtag accaccagag ggaaatcatc
    27841 ctctggctgg cggtttctgg tatttcggga caagtacgcg cacgcgtagc acccaatcgg
    27901 tgaggaaata gcggcgacct gaatggtcaa aatcacaacg cagctatccc cgtaagcatc
    27961 gcttaacgga atagcagtaa cctgaatcca gtggggaggg ggtagtcaaa tctctacagg
    28021 cctgactatc cgggactgcc cgccccatcg ttttttatac ccgcgaaaaa tgaaatttaa
    28081 tccgggcgtg tttcatcctt ccagagggta tgacgtatga caccacgcga aatagcctta
    28141 ttgaccatcg ccaaacttga gcacggaggc caccagctta cacaggcaga tcaacgggag
    28201 atagaacgat cagttaatgc cgatatagcc cggcgcgaca ggttccgcga aatgatgcga
    28261 gcacctgcct accagtggaa gaagccagcg ccgcgcaggt agatatggga ttattccaca
    28321 tgtaacttat gcgtattaag caaatttc
//
LOCUS       DBANUU010000055        28252 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000055 DBANUU010000000
VERSION     DBANUU010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.055"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            204..1151
                     /gene="cyoA"
                     /locus_tag="ACIE68_002307"
     CDS             204..1151
                     /gene="cyoA"
                     /locus_tag="ACIE68_002307"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFX5908614.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            1173..3164
                     /gene="cyoB"
                     /locus_tag="ACIE68_002308"
     CDS             1173..3164
                     /gene="cyoB"
                     /locus_tag="ACIE68_002308"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFX5908615.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            3154..3768
                     /locus_tag="ACIE68_002309"
     CDS             3154..3768
                     /locus_tag="ACIE68_002309"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFX5908616.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3768..4097
                     /locus_tag="ACIE68_002310"
     CDS             3768..4097
                     /locus_tag="ACIE68_002310"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFX5908617.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            4109..4999
                     /gene="cyoE"
                     /locus_tag="ACIE68_002311"
     CDS             4109..4999
                     /gene="cyoE"
                     /locus_tag="ACIE68_002311"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFX5908618.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            5148..6512
                     /locus_tag="ACIE68_002312"
     CDS             5148..6512
                     /locus_tag="ACIE68_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5908619.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(6640..7131)
                     /gene="yajQ"
                     /locus_tag="ACIE68_002313"
     CDS             complement(6640..7131)
                     /gene="yajQ"
                     /locus_tag="ACIE68_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFX5908620.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            7299..8210
                     /gene="panE"
                     /locus_tag="ACIE68_002314"
     CDS             7299..8210
                     /gene="panE"
                     /locus_tag="ACIE68_002314"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFX5908621.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVATPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8173..8763
                     /gene="yajL"
                     /locus_tag="ACIE68_002315"
     CDS             8173..8763
                     /gene="yajL"
                     /locus_tag="ACIE68_002315"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFX5908622.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(8817..10265)
                     /gene="thiI"
                     /locus_tag="ACIE68_002316"
     CDS             complement(8817..10265)
                     /gene="thiI"
                     /locus_tag="ACIE68_002316"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFX5908623.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            10471..10713
                     /gene="xseB"
                     /locus_tag="ACIE68_002317"
     CDS             10471..10713
                     /gene="xseB"
                     /locus_tag="ACIE68_002317"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFX5908624.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            10713..11612
                     /gene="ispA"
                     /locus_tag="ACIE68_002318"
     CDS             10713..11612
                     /gene="ispA"
                     /locus_tag="ACIE68_002318"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFX5908625.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            11637..13499
                     /gene="dxs"
                     /locus_tag="ACIE68_002319"
     CDS             11637..13499
                     /gene="dxs"
                     /locus_tag="ACIE68_002319"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFX5908626.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            13679..14653
                     /gene="yajO"
                     /locus_tag="ACIE68_002320"
     CDS             13679..14653
                     /gene="yajO"
                     /locus_tag="ACIE68_002320"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFX5908627.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSFSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGRNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(14707..15225)
                     /gene="pgpA"
                     /locus_tag="ACIE68_002321"
     CDS             complement(14707..15225)
                     /gene="pgpA"
                     /locus_tag="ACIE68_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFX5908628.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(15203..16180)
                     /gene="thiL"
                     /locus_tag="ACIE68_002322"
     CDS             complement(15203..16180)
                     /gene="thiL"
                     /locus_tag="ACIE68_002322"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFX5908629.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(16258..16677)
                     /gene="nusB"
                     /locus_tag="ACIE68_002323"
     CDS             complement(16258..16677)
                     /gene="nusB"
                     /locus_tag="ACIE68_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFX5908630.1"
                     /translation="MKPAARRRAREFAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(16697..17167)
                     /gene="ribE"
                     /locus_tag="ACIE68_002324"
     CDS             complement(16697..17167)
                     /gene="ribE"
                     /locus_tag="ACIE68_002324"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFX5908631.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(17256..18359)
                     /gene="ribD"
                     /locus_tag="ACIE68_002325"
     CDS             complement(17256..18359)
                     /gene="ribD"
                     /locus_tag="ACIE68_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFX5908632.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(18363..18812)
                     /gene="nrdR"
                     /locus_tag="ACIE68_002326"
     CDS             complement(18363..18812)
                     /gene="nrdR"
                     /locus_tag="ACIE68_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFX5908633.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            18963..19502
                     /locus_tag="ACIE68_002327"
     CDS             18963..19502
                     /locus_tag="ACIE68_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFX5908634.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            19801..20685
                     /gene="tsx"
                     /locus_tag="ACIE68_002328"
     CDS             19801..20685
                     /gene="tsx"
                     /locus_tag="ACIE68_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFX5908635.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINDIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(20862..21209)
                     /gene="yajD"
                     /locus_tag="ACIE68_002329"
     CDS             complement(20862..21209)
                     /gene="yajD"
                     /locus_tag="ACIE68_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFX5908636.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(21338..22309)
                     /gene="secF"
                     /locus_tag="ACIE68_002330"
     CDS             complement(21338..22309)
                     /gene="secF"
                     /locus_tag="ACIE68_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFX5908637.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(22320..24167)
                     /gene="secD"
                     /locus_tag="ACIE68_002331"
     CDS             complement(22320..24167)
                     /gene="secD"
                     /locus_tag="ACIE68_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFX5908638.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(24195..24527)
                     /gene="yajC"
                     /locus_tag="ACIE68_002332"
     CDS             complement(24195..24527)
                     /gene="yajC"
                     /locus_tag="ACIE68_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFX5908639.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(24550..25677)
                     /gene="tgt"
                     /locus_tag="ACIE68_002333"
     CDS             complement(24550..25677)
                     /gene="tgt"
                     /locus_tag="ACIE68_002333"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFX5908640.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(25733..26803)
                     /gene="queA"
                     /locus_tag="ACIE68_002334"
     CDS             complement(25733..26803)
                     /gene="queA"
                     /locus_tag="ACIE68_002334"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFX5908641.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            26896..27477
                     /gene="acpH"
                     /locus_tag="ACIE68_002335"
     CDS             26896..27477
                     /gene="acpH"
                     /locus_tag="ACIE68_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFX5908642.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(27482..>28252)
                     /locus_tag="ACIE68_002336"
     CDS             complement(27482..>28252)
                     /locus_tag="ACIE68_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFX5908643.1"
                     /translation="NNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAMN
                     YRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDTA
                     RFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQDT
                     ALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEVV
                     LPASPLLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
BASE COUNT         6691 a         7481 c         7239 g         6841 t
ORIGIN      
        1 agactccttc tgcactcccc ttttgttata acgccctttt gcaacagctt cttaaaatca
       61 acctgatatg ttttgcaaca tatgtgacct gacagccaaa tccaagtaac aggaatttaa
      121 tcatgtttac agtaatgtaa ccttcccgta aaatgcccac acactttaaa cgccaccaga
      181 tcccgtggaa ttgaggtcgt taaatgagac tcaggaaata caataaaagt ttgggatggt
      241 tgtcattatt tgcaggcact gtattgctca gtggctgtaa ttctgcgctg ttagatccca
      301 aaggacagat tggtctggag caacgttcac tgatactgac ggcatttggc ctgatgttga
      361 ttgtcgttat tcccgcaatc ttgatggctg ttggtttcgc ctggaagtac cgtgcgagca
      421 ataaagatgc taagtacagc ccgaactggt cacactccaa taaagtggaa gctgtggtct
      481 ggacggtacc tatcttaatc atcatcttcc ttgcggtact gacctggaaa accactcacg
      541 ctcttgagcc tagcaagccg ctggcacacg acgagaagcc cattaccatc gaagtggttt
      601 ccatggactg gaaatggttc ttcatctacc cggaacaggg cattgctacc gtgaatgaaa
      661 tcgctttccc ggcgaacact ccggtgtact tcaaagtgac ctccaactcc gtgatgaact
      721 ccttcttcat tccgcgtctg ggtagccaga tttatgccat ggccggtatg cagactcgcc
      781 tgcatctgat cgccaacgaa cccggtactt atgacggtat ctccgccagc tacagcggcc
      841 cgggcttctc aggcatgaag ttcaaagcta ttgcaacacc ggatcgcgcc gcattcgacc
      901 aatgggtcgc aaaagcgaaa cagtcgccga actccatgtc tgacatggca gcgttcgaaa
      961 aactggccgc gcctagcgaa tacaaccagg tggaatattt ctccaacgtg aaaccagact
     1021 tgtttgccga tgtgattaac aagtttatgg ctcacggtaa gagcatggac atgacccagc
     1081 cagaaggtga gcacagcgca cacgaaggta tggaaggcat ggacatgagc cacgcggaat
     1141 ccgcccatta aaggggttga ggaagaataa agatgttcgg aaaattatca cttgatgcag
     1201 tcccgttcca tgaacctatc gtcatggtta cgatcgctgg cattattttg ggaggtctgg
     1261 cgctcgttgg cctgatcact tacttcggta agtggaccta cctgtggaaa gagtggctga
     1321 cctccgtcga ccataaacgc ctcggtatca tgtatatcat cgtggcgatt gtgatgttgc
     1381 tgcgtggttt tgctgacgcc attatgatgc gtagccagca ggctcttgcc tcggcgggcg
     1441 aagcgggctt cctgccacct caccactacg atcagatctt taccgcgcac ggcgtgatta
     1501 tgatcttctt cgtggcgatg cctttcgtta tcggtctgat gaacctggtg gttccgctgc
     1561 agattggcgc gcgtgacgtt gcgttcccgt tcctcaacaa cttaagcttc tggtttaccg
     1621 ttgttggtgt gattctggtt aacgtttctc tcggcgtggg cgaatttgcg cagaccggct
     1681 ggctggccta tccaccgcta tcgggaatag agtacagtcc gggagtcggt gtcgattact
     1741 ggatatggag tctccagcta tccggtatag gtacgacgct taccggtatc aacttcttcg
     1801 ttaccattct gaagatgcgc gcaccgggca tgaccatgtt caagatgcca gtatttacct
     1861 gggcatcact gtgcgcgaac gtactgatta ttgcttcctt cccaattctg acggttaccg
     1921 tcgcgttgtt gaccctggat cgctatctgg gcacccattt ctttaccaac gatatgggtg
     1981 gcaacatgat gatgtatatc aacctgattt gggcctgggg ccacccggaa gtttacatcc
     2041 tgatcctgcc tgttttcggt gtgttctccg aaattgcggc aaccttctcg cgtaaacgtc
     2101 tgtttggtta tacctcgctg gtatgggcaa ccgtctgtat caccgtgctg tcgttcatcg
     2161 tctggctgca ccacttcttt acgatgggtg cgggcgcgaa cgtaaacgcc ttctttggta
     2221 tcaccaccat gattatcgcc atcccgaccg gggtgaagat cttcaactgg ctgttcacca
     2281 tgtatcaggg ccgcatcgtg ttccattctg cgatgctgtg gactatcggt tttatcgtca
     2341 ccttctcggt gggcggtatg actggcgtgc tgctggcagt accgggcgcg gacttcgttc
     2401 tgcataacag tctgttcctg attgcgcact tccataacgt gatcatcggc ggcgtggtct
     2461 tcggctgctt cgcagggatg acctactggt ggcctaaagc gttcggtttc aaactgaacg
     2521 aaacctgggg taaacgcgcg ttctggttct ggatcatcgg cttcttcgtt gcctttatgc
     2581 cactgtatgc gctgggcttc atgggtatga cccgccgttt gagccagcag attgacccgc
     2641 agttccacac catgctgatg attgcagcca gcggtgcagt actgattgcg ctgggtattc
     2701 tctgcctcgt tattcagatg tacgtttcta ttcgtgaccg cgaccagaac cgtgacctga
     2761 ctggcgaccc gtggggtggc cgtacgctgg agtgggcaac ctcttccccg cctccgttct
     2821 ataactttgc cgttgtgccg cacgttcacg agcgtgatgc attctgggaa atgaaagaga
     2881 aaggcgaagc gtacaaaaag cctgaccact atgaagaaat tcatatgccg aaaaacagcg
     2941 gtgcaggtat cgtcattgca gctttctcca ccatcttcgg tttcgccatg atctggcata
     3001 tctggtggct ggcgattgtt ggcttcgcag gcatgatcat cacctggatc gtgaaaagct
     3061 tcgacgagga cgtggattac tacgtgccgg tggcagaaat cgaaaaactg gaaaaccagc
     3121 atttcgatga gattactaag gcagggctga aaaatggcaa ctgatacttt gacgcacgcg
     3181 actgcccacg cgcacgaaca cgggcaccac gatgcaggcg gaaccaaaat cttcggattt
     3241 tggatctacc tgatgagcga ctgcattctg ttctctatct tgtttgctac ctatgccgtt
     3301 ctggtgaacg gcaccgcagg cggcccgaca ggtaaggaca ttttcgaact gccgttcgtt
     3361 ctggttgaaa ctttcttgct gttgttcagc tccatcacct acggcatggc ggctatcgcc
     3421 atgtacaaaa acaacaaaag ccaggttatc tcctggctgg cgttgacctg gttgtttggc
     3481 gccggattta tcgggatgga aatctacgaa ttccatcacc tgattgttaa cggcatgggt
     3541 ccggatcgca gcggcttcct gtcagcgttc tttgcgctgg tcggcacgca cggtctgcac
     3601 gtcacttccg gtcttatctg gatggcggtg ctgatggtac aaatcgctcg tcgcggcctg
     3661 accagcacta accgtacccg catcatgtgc ctgagcctgt tctggcactt cctggatgtg
     3721 gtttggatct gtgtgttcac tgttgtttat ctgatggggg cgatgtaatg agtcattcta
     3781 ccgatcacag cggcgcgtcc catggcagcg taaaaaccta catgacaggc tttatcctgt
     3841 cgatcattct gacggtgatt ccgttctgga tggtgatgac aggggctgcc tctccggccg
     3901 taattctggg aacaatcctg gcaatggcag tggtacaggt tctggtgcat ctggtgtgct
     3961 tcctgcacat gaataccaaa tcagatgaag gctggaacat gacggcgttt gtcttcaccg
     4021 tgctaatcat cgctatcctg gttgtaggct ccatctggat tatgtggaac ctcaactaca
     4081 acatgatgat gcactaagag cggcggttat gatgtttaag caatacctgc aagtaacgaa
     4141 accaggcatc atctttggca acctgatctc ggtgattggg ggattcctgc tggcctcaaa
     4201 gggcagcatt gattatcccc tgtttatcta cacgctggtt ggggtgtcac tggttgtggc
     4261 gtcgggttgt gtgtttaaca actacatcga cagggatatc gacagaaaga tggaaaggac
     4321 gaagaatcgg gtactggtta aaggcctgat ctctcctgct gtctcgctgg tgtacgccac
     4381 cttgctaggc tttgctggct ttatgctgct gtggtttggc gcgaatccgc tggcctgctg
     4441 gctgggggtg atgggctttg tcgtttatgt cggcgtttat agcctgtaca tgaaacgcca
     4501 ctcagtctac ggcacattga ttggttcgct ctccggcgct gcgccgccgg tgattggcta
     4561 ctgtgcagta accggcgagt tcgatagcgg cgcagcgatc ctgctggcta tcttcagcct
     4621 gtggcagatg cctcactcct atgccatcgc cattttccgc tttaaggatt atcaggcggc
     4681 gaacattccg gtattgccag tggtaaaagg catttcggtg gcgaagaatc acatcacgct
     4741 gtatatcatc gcctttgccg ttgccacgct gatgctctct cttggcggtt acgctggata
     4801 taaatatctg gtggtcgccg cggcggttag cgtctggtgg ttaggtatgg ctctgcgcgg
     4861 ttataaagtt gctgatgaca gaatctgggc gcgcaagctg ttcggcttct ctatcatcgc
     4921 catcactgcc ctctcggtga tgatgtccgt tgattttatg gtaccggact cgcatacgct
     4981 gctggctgct gtgtggtaac aaaacctctc agttaaaaag gtgctacggc accttttttc
     5041 ttagcattag aaacatatcc ctctcgaaat atttactaaa aaatccgcat gtttacccca
     5101 ttcgtttgcc gctttacact agtcgcgaat ttaaaacaga ggtggtaatg aacgattata
     5161 aaatgacgcc aggtgagcgg cgcgcgacct ggggtttagg aaccgttttc tcgttgcgca
     5221 tgctgggcat gtttatggtt ctgccggttc tgaccacgta tggcatggct ctgcaaggtg
     5281 ccagcgaagc attaatcggt attgccattg gtatttatgg tctgactcag gccgtttttc
     5341 agattccgtt tggcctgctt tccgaccgca ttggtcgcaa accattaatt gtcggtgggc
     5401 tggcggtgtt tgccgccggt agcgttatcg ctgcgctttc tgactccatc tggggaatta
     5461 ttctgggccg ggcgctacaa ggctccggtg cgattgccgc tgccgttatg gcgctgcttt
     5521 ccgatctcac gcgcgaacaa aaccgtacca aagcaatggc gtttatcggc gtgagctttg
     5581 gcattacctt tgccattgcg atggtgcttg gcccgatcat cactcacaaa cttgggctgc
     5641 acgcgctgtt ctggatgatc gctattctgg caacaaccgg cattgcgttg accatttggg
     5701 ttgtgcccaa cagtagcact cacgtactta atcgtgagtc cggaatggtg aaaggcagtt
     5761 tcagtaaagt gctggcggaa ccgcggctgc taaaactcaa ctttggcatt atgtgtctgc
     5821 acatgcttct gatgtctacg tttgttgccc tgcccggaca gctagctgat gcggggttcc
     5881 cggcggctga acactggaag gtctatctgg cgacgatgtt aatcgccttt ggctcggtcg
     5941 tgcctttcat tatctacgct gaagttaagc gcaaaatgaa gcaagtcttt gtcttctgcg
     6001 tcgggttgat cgtggttgcg gaaattgtgt tgtggaacgc acaaacgcag ttctggcaac
     6061 tggtggtcgg cgtgcagctt ttctttgtgg cgtttaattt gatggaagcc cttctgccct
     6121 cacttatcag taaagagtcg ccagcaggtt acaaaggtac ggcgatgggt gtctactcca
     6181 ccagccagtt tcttggcgtg gcgattggcg gttcgctggg cggctggatt gacggcatgt
     6241 ttgacggtca gggggtattt ctcgctggcg caatgctggc cgcagtgtgg ctggcagtcg
     6301 ccagtaccat gaaagaaccg ccgtatgtca gcagtttgcg cattgaaatc ccggcggaca
     6361 ttgccgcaaa cgaagcgtta aaagtgcgtt tgctggaaac tgaaggcgtc aaagaagtgt
     6421 tgattgcaga agaagagcat tcagcttatg tgaaaatcga cagcaaagtg acaaatcgct
     6481 ttgaggttga acaggcaatt cgtcaggcat aaaaaacgcg gcgaattgcc tgctgcgcta
     6541 cgcttaccag gcctacaaag ttcttgagtt acaaaccgtt gtaggccgaa tcaggcgttc
     6601 atgccgcatc tgacatgaac aaaacgcaca ttgtcgcgat taatcgcgga agtttttgaa
     6661 ctggaacggc tggccgagat cgccaccacg taccatcgcc atgacagcct gcaaatcatc
     6721 acgagatttg ccagtcacac ggatctcatc gccctgaatt tgcgcctgca ctttcagttt
     6781 gctgtcttta atcatcttga cgattttctt ctgggtcgcg ctctcaatgc cctgtttcag
     6841 tttcgcttcc acaaaccagg ttttaccgct atgaacgata ttttccggta catccagtga
     6901 actgccttca atgccgcgct tcagcagctt ggcacgcaga atatccagca actgattgac
     6961 ctggaagtcg gactcgctca acactttgat ggttttgctg gcgtcgttca gctcaaatga
     7021 ggcttcaacg ttacggaagt caaaacggga ctccacttcg cgactcgcgt tatcgaccgc
     7081 gttacgtgct tcctgaagat caacttcaga gacaatatcg aaagatggca tcttttcctc
     7141 tcccttcatt tttgatgcga agcataatac ctgcaaagtt aaataaccaa cagtcctgca
     7201 ccaggacgta tcccttaatt gtgcatagtc gcaacagcat gcacaattaa gggatagcct
     7261 tattgaacac ccggagtggt tgcgggtgag gaggaacaat gaaaattacc gtattgggat
     7321 gcggtgcctt agggcaatta tggcttacag cactttgtaa acagggtcac gaagttcagg
     7381 gctggctgcg cgtaccgcaa ccttattgta gtgtgaatct ggttgagaca gatggttcga
     7441 tatttaacga atcgctgacc gccaacgatc ccgattttct cgccaccagc gatctgctcc
     7501 tggtgacgct gaaagcatgg caggtttccg atgccgtcaa aagcctcgca tccacactgc
     7561 ctgtagctac gccaatactg ttaattcaca acggcatggg caccatcgaa gaattgcaaa
     7621 acattcagca gccattactg atgggcacca ccacccatgc cgcccgccgc gacggcaatg
     7681 tcattattca tgtggcaaac ggaatcacgc atatcggtcc ggcacggcaa caggatggcg
     7741 attacagtta tctggcggat attttgcaaa ccgtgttgcc tgacgttgcc tggcataaca
     7801 atattcgcgc cgagctgtgg cgcaagctgg ccgtcaactg tgtgattaat ccactgaccg
     7861 ccatctggaa ttgcccgaat ggtgaattac gtcatcatcc gcaagaaatt atgcagatat
     7921 gcgaagaagt cgcggcagtg atcgagcgcg aagggcatca tacttcagca gaagatttgc
     7981 gtgattacgt gatgcaggtg attgatgcca cagcggaaaa tatctcgtcg atgttgcagg
     8041 atatccgcgc gctgcgccac accgaaatcg actatatcaa tggttttctc ttacgccgcg
     8101 cccgcgcgca tgggattgcc gtaccggaaa acacccgcct gtttgaaatg gtaaaaagaa
     8161 aggagagtga atatgagcgc atcggcactg gtttgcctcg cccctggtag tgaagagact
     8221 gaagccgtca ccactatcga tctgctggtt cgcggcggta tcaaagtcac cactgccagc
     8281 gtcgccagcg atggcaacct ggccattacc tgctcgcgcg gcgtgaagct gctggcggat
     8341 gcgccgctgg tcgaggtggc tgatggcgaa tatgacgtga tcgtactgcc tggtggcatt
     8401 aaaggcgcag agtgttttcg cgatagtacc ctgctggttg aaaccgttaa acagttccac
     8461 cgttccgggc gtatcgtcgc ggctatttgc gccgcgccag ccaccgtgct ggtgccgcac
     8521 gatatcttcc cgattggtaa tatgaccggc ttcccgacgc tgaaagacaa aattcccgct
     8581 gaacaatggc aggacaagcg cgtcgtctgg gatgcacggg taaaattgct gaccagccag
     8641 gggccgggga cagctatcga ctttggtctg aaaattatcg acctgctggt tgggcgcgag
     8701 aaagcccatg aagtggcatc acaactggtg atggcggcag ggatttataa ttattacgag
     8761 tagtgtcgga tgcggtaagc gtcgcatctg accagatgcg acgttacaac cacaaattac
     8821 gggcgatata ccttcacatt gttaaagccc tgctcgcgca gatagagcgc ctgcagacgg
     8881 ctcatcaccc cgcgctcaca ccacagtagc caggttctgt tctggtcgag atcgccaaac
     8941 ttggtgctca gtttatagaa cggcagagaa actacatcaa tcccttcgac tttcagtggc
     9001 ttatcttcct gttcatcgat agaacggata tcgaggatca cgtcgttcgg gccgaagcca
     9061 ttgacggttt ccacttccac cacttcctgc tcggtctgct gggcgatttc gcggatatca
     9121 acgttattcg cttcttcaac cactttatcg agaatgctga agtcgaattt ctcttcttcc
     9181 gcttcaatct tcgacttaac cgctttcacc gtcgggcttt tggagataac accgcaatat
     9241 tccggcatcg tgcgagcaaa gtcttcggtg ccaatctggc gagccaggtt gatgatgtgc
     9301 tctttgtcgt aagaaatcag cggacgcagg atcagcgtat cggagacgtt atcaatcagg
     9361 cgcaggttgg tcagcgtctg actggacacc tggccgagcg cttcgccggt gaccagcgcc
     9421 tgtacgccgt aacgttcagc cactttagac gcggcacgca ccatcatacg tttgaggata
     9481 acgcccatct gaccgtcgtc gattttctcg agaatttccc cgacgaccgg ttcgaaatta
     9541 atagcgacaa aacgcacgcg gtgggagctg ccaaaacggt tccacagata atgcgccacc
     9601 tgacgcacgc caatttcatg cgccgcgccg ccaaggttaa agaaacagta atgcacgcgg
     9661 cagccgcgac gcatcaacat ataactggaa acaccggagt cgaaaccacc ggaaatgagc
     9721 gacagcacat cttcctgggt gccgatcggg aaaccgccaa taccttcgta gcggccttta
     9781 atcagcagga gacgatcgtc ttccacttcc agatggacag tcacttccgg attggtcagc
     9841 ttcacgcgcg cggactcaat atgctgattt aaaccgccgc cgacgtaacg ttccacatca
     9901 atcgagctaa aatcatgttt gccacggcgc ttcacgcgta cgcagaaggt tttgccttcc
     9961 agctgatcgc gatactgaac caacgctttc tcaaaaatat cgtgcatgtc ggtaaacggc
    10021 acgtcttcga cttcgagaat atggtggata cccggaatac gggtcagggc gtcgcgaata
    10081 gccagacgct ggttttcatc ttttgcgcga acttcgatgt tatcccagtg gcggacgaca
    10141 gcgagcgtct catcatagtg ctttaaaacg ttacgaatgt tcccggtaag gatttttata
    10201 aagcgcaagc gcacagattg gcttttgatg gtgatttccg ggaacaattt aatgataaac
    10261 ttcatggcgg caatggttcg ttggcaagcc ttaagcgact tgtataggga aaaatacagc
    10321 agcccacacc tgcggctgca tccaggcgcg gaagtatacc actaacatcg ctttgctgcg
    10381 cacatcacct taccattgcg cgttatttgc tatttgccct gagtccgtta ccatgacggg
    10441 gcgaaaaata ttgagagtca gacattcatt atgccgaaga aaaatgaggc tcccgccagc
    10501 tttgaaaagg cgctgagcga gctggaacag attgtaaccc gtctggaaag tggcgacctg
    10561 ccgctggaag aggcgctgaa cgagttcgaa cgcggcgtgc agctggcacg tcaggggcag
    10621 gccaaattac aacaagccga acagcgcgta caaattctgc tgtctgacaa tgaagacgcc
    10681 tctctaaccc cttttacacc ggacaatgag taatggactt tccgcagcaa ctcgaagcct
    10741 gcgttaagca ggccaaccag gcgctgagcc gttttatcgc cccactgccc tttcagaaca
    10801 ctcccgtggt cgaaaccatg cagtatggcg cattattagg tggtaagcgt ctgcgaccct
    10861 tcctggttta tgccaccggt catatgtttg gcgttagcac aaacacgctg gacgcacccg
    10921 ctgccgccgt agagtgtatc cacgcttact cattaattca tgatgattta ccggcgatgg
    10981 atgatgacga tctgcgtcgc ggtttgccga cctgccatgt gaagtttggc gaagcaaacg
    11041 cgattctcgc tggcgacgct ttacaaacgc tggcgttctc gattctaagc gatgccaata
    11101 tgccggaagt gtcggatcgc gacagaattt cgatgatttc tgaactggcg agcgccagcg
    11161 gtattgccgg aatgtgcggt ggtcaggcat tagatttaga cgcggaaggc aaacacgtac
    11221 ctctggacgc gcttgagcgt attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg
    11281 ttcgccttgg tgcattaagc gccggagata aagggcgtcg tgctctgcca gtactcgaca
    11341 agtacgcaga gagcatcggc cttgccttcc aggttcagga tgacatcctg gatgtggtag
    11401 gagatactgc aacgttggga aaacgccagg gtgccgacca gcaacttggt aaaagtacct
    11461 accctgcact tctgggtctt gagcaagccc ggaagaaagc ccgggatctg atcgacgatg
    11521 cccgccagtc gctgaaacaa ctggctgaac aatcactcga tacctcggca ctggaagcgc
    11581 tagcggacta catcatccag cgtaataaat aaacaataag tattaatagg cccctgatga
    11641 gttttgatat tgccaaatac ccgaccctgg cactggtcga ctccacccag gagttacgac
    11701 tgttgccgaa agagagttta ccgaaactct gcgacgaact gcgccgctat ttactcgaca
    11761 gcgtgagccg ttccagcggg cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg
    11821 cgctgcacta tgtctacaac accccgtttg accaattgat ttgggatgtg gggcatcagg
    11881 cttatccgca taaaattttg accggacgcc gcgacaaaat cggcaccatc cgtcagaaag
    11941 gcggcctgca cccgttcccg tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc
    12001 attcatcaac ctccatcagt gccggaattg gtattgcggt tgctgccgag aaagaaggca
    12061 aaaatcgccg caccgtctgt gtcattggcg atggcgcgat taccgctggc atggcgtttg
    12121 aagcgatgaa tcacgcgggc gatatccgtc ctgatatgct ggtggtcctc aacgacaatg
    12181 aaatgtcgat ttccgaaaat gtcggcgcgc tcaataacca tctggcacag ctgctttccg
    12241 gtaagcttta ctcttcgctg cgcgaaggcg ggaaaaaagt tttctctggc gttccgccaa
    12301 ttaaagagct gctcaaacgt accgaagaac atattaaagg catggtagtg cctggcacgt
    12361 tgtttgaaga gctgggcttt aactacatcg gcccggttga cggtcacgat gtgctggggc
    12421 ttatcaccac gctgaagaac atgcgcgacc tgaaaggccc gcagttcctg catatcatga
    12481 ccaaaaaagg tcgtggttat gaaccggcag aaaaagaccc catcactttc cacgccgtgc
    12541 ctaaatttga tccctccagc ggttgtttgc cgaaaagtag cggcggtttg ccgagctatt
    12601 caaaaatctt tggcgactgg ttgtgcgaaa cggcagcgaa agacaacaag ctgatggcga
    12661 ttactccggc gatgcgtgaa ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc
    12721 gttacttcga cgtggcaatc gccgagcaac acgcggtgac ctttgccgcc ggtctggcga
    12781 ttggtgggta caaacccatt gtcgcgattt actccacttt cctgcaacgc gcctatgatc
    12841 aggtgctgca tgacgtggcg attcaaaagc tcccggtcct gttcgccatc gaccgcgcgg
    12901 gcattgttgg tgctgacggt caaacccatc agggcgcttt tgacctctct tacctgcgct
    12961 gtataccgga aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct
    13021 ataccggcta tcactataac gacggcccgt ccgcggtgcg ctacccgcgc ggtaacgcgg
    13081 ttggcgtgga actgacgccg ctggaaaaac tgccaattgg caaaggcatt gtgaagcgtc
    13141 gtggtgagaa actggcgatc cttaactttg gtacgctgat gccagaagcg gcgaaagtcg
    13201 ccgaatcgct gaacgccacg ctggtcgata tgcgttttgt gaaaccgctt gatgaagcgt
    13261 taattctgga aatggccgcc agccatgaag cgctggtcac cgtagaagaa aacgccatta
    13321 tgggcggcgc aggtagcgga gtgaacgaag tgctgatggc ccatcgtaaa ccagtacccg
    13381 tgctgaacat tggtctgccg gacttcttta ttccgcaagg aactcaggaa gaaatgcgcg
    13441 ccgaactcgg cctcgatgcc gccggtatgg aagccaaaat caaggcctgg ctggcataat
    13501 ccctactcca ctcctgctat gcttaacaaa ttattcatag actctaaata attcgagttg
    13561 caggaaggcg gcaaacgagt gaagccccag gagcttacat aagtaagtga ctggggtgaa
    13621 cgaatgcagc cgcagcacat gcaacttgaa gtatgacgag tatggcagga gtggcagcat
    13681 gcaatacaac cccttaggaa aaaccgacct tcgcgtttcc cgactttgcc tcggctgtat
    13741 gacctttggc gagccagatc gtggtaatca cgcatggaca ctgccggaag agagtagccg
    13801 tcccatcatt aaacgcgcgc tggaaggcgg cataaatttc tttgataccg ccaatagctt
    13861 ttccgatggc agcagcgaag agatcgttgg tcgcgcactg cgggatttcg cccgtcgtga
    13921 agacgtggtc gttgcgacga aagtgttcca tcgcgttggt gatttaccgg aaggattatc
    13981 ccgtgcacaa attttgcgct ctatcgacga cagcctgcgc cgtctcggca tggattatgt
    14041 cgatatcctg caaattcatc gctgggatta caacacgccg atcgaagaga cgctggaagc
    14101 cctgaacgac gtggtaaaag ccgggaaagc gcgttatatc ggcgcgtcat ccatgcacgc
    14161 ttcgcagttt gctcaggccc tgaagctaca aaaacagcac ggctgggcgc agtttatcag
    14221 tatgcaggat cactacaatc tgatttatcg cgaagaagag cgcgagatgc tgcctctgtg
    14281 ttatcaggag ggcgtggcgg tgattccgtg gagcccgctg gcgcgggggc gactgacgcg
    14341 tccgtgggga gaaactaccg cacgactggt gtctgatgag gtggggagaa atctctataa
    14401 agaaagcgat gaaaatgacg cgcagatcgc agagcggtta acgggcgtca gtgaagaact
    14461 cggtgcaaca cgagcacaag ttgcgctggc ctggttgttg agtaaaccgg gcattgccgc
    14521 accgattatc ggtacatcgc gggaagaaca gcttgatgag ctgctgaacg cggtggatat
    14581 cactttgaag ccagaacaga ttgccgaact ggaaacgccg tataaaccgc atgcggtcgt
    14641 aggatttaaa taagagtcca ggcctgatga gacgtgacaa gcgtcacatc aggcatcggt
    14701 gcacaactac gacagaatac ccagcggcca gtgatgaccg ataaagtaca ggatgcctgc
    14761 ggaaatcacc ccggcgacaa tatcgtcgat catgatcccc atgccgccat gtacattgcg
    14821 atcaaaccag cggatcggcc acggcttcca catatccaga atacggaaaa tcacaaaccc
    14881 ggcggcaacc cactgccagt cattggtcgg cagcgccatg agcgtgatcc acataccaat
    14941 aaattcgtcc cagacaatgc tgccgtgatc gtgcacgccc atatctttcg ccgtttgatg
    15001 acaaagatag acgccgatac agatccccaa catcaccacc agcgagtaga gctgccaggg
    15061 taaaaaggtc atcagatacc agaacggaat cgctgccagc gatcccatcg tgccaggaac
    15121 gattgggctt aatccacttc cgaatccgac agcaagcaga tgccacggat tactcatctt
    15181 caggcgactt ttcgcgacat ctttatggcg tggcaaaatg gtcatatcct ttccagtcaa
    15241 acgtgacagg ttcgccgtca cgaataaaac aaagcccttc gatatcggcg gtcatttgcc
    15301 cgatacaggt aaacggtacg cccaggtgtc cgagagccac atccagcgcg ccacggttca
    15361 gttccggcac agtgaaacac aactcgtaat cttcaccgcc agagagcgcc cagcgcagcg
    15421 cctgttccgg ttcaacatgg cgagaaagcg catcagaaaa cggcagcaat gccaggtcaa
    15481 tacgtgcgcc gcagtcgctg gctttcacga tatgcccgag atcggaaatc agaccgtcag
    15541 agagatcgat agctgaattt gccagatcgc gcagtgcctg tccctgtaaa atacgcggcg
    15601 atggacggag atgacgtttg atcaagtagt ccgcatcttt agcatcggca acctgcaaac
    15661 ggttttgcaa aatcgccagc ccggcggcgc tatcgcccgg tgtaccggtc acatagatcc
    15721 agtcacccgg tttcgctcca gagcgcgtta aggctcgtcc catcggaaca aagccgtgga
    15781 tacccaacgt cattgataat ggcccacgcg tggtatcgcc gccaatgagt tgcatatcgt
    15841 aataattgag aagatcaaac aaactgtcgc tgaaggactc aagccacgct tcgtctacgt
    15901 ccggtaaggt taatgccagc gtcagccagg ccggatcggc ccccatcgct gccagatcgc
    15961 ttaggttcac cgccagtgct ttatacgcca gatcagcagg atcgatatca tggaggaagt
    16021 ggttgcccgc caccagcgta tcagtgctga tcgccagggt ctgtttctcg gggatattga
    16081 gaagtgcgca atcgtcgccg atgcccagtt cgacatcaag acgagaactt cttacacggt
    16141 caaaataacg ggcaatcagg gagaactcgc cacatgccat acgttatgcc tcagcaggta
    16201 aaaaagaaaa ggccggcgat catggaacgg tcttccgtga atctaccggc ctggatatta
    16261 ctttttgtta gggcgaatca caggtgctgc tttatcgagt acgccgttga cgaacttatg
    16321 gctgtcttct gcgccgaacg atttcgccag ttcgatcgct tcgttaatgg ccactttgta
    16381 tggcacatcg ctacgtttag acagctcgta cagcgcaatg cgcagtactg ctttttctac
    16441 ctgtcccagt tcttccagca ggcgggacag gtatggcttc atcagtccgt cgaggtatgc
    16501 ggtgttagtc gccaccccgg ccagcagctc acggaagtac aggacgtcaa catcttttac
    16561 atcctgttca gccaggaact ggtattcaac atcagcgatg tcgttctggg acaactgcca
    16621 ggagtagagc gcctggacgg caaactcacg agcgcggcga cgagcagcag gtttcacgga
    16681 tttcccctta ctaatttcag gccttgatgg ctttcaatac attaatcatt tcaagcgcgg
    16741 tcagtgcagc ttctgcacct ttgttgccag ctttggtgcc agcacgttcg atcgcttgtt
    16801 caatgctttc agtggtcaga accccaaaag caaccggaat ttcgctgtcc tgggcaacat
    16861 gcgccaggcc gttgcttgca ccaccagcga catattcaaa atgggcagtg ccaccacgaa
    16921 taaccgtacc cagcgcaatc accgcgtcgt atttaccggt tttagccagt gcacccgccg
    16981 ccagcggcag ctcataggca ccaggcaccc aaacaacggt aatgttttca tcttttacct
    17041 gaccgatacg tttcagtgcg tcaattgcac cttccagcag gctgtcattg ataaagttgt
    17101 tgaaacgcgc gatggtgatg gcgacgcgag cgtccggggt agcaacgtta gcttcaataa
    17161 tgttcatttt cttccttcgg gttcgagtat ggccccgcag gggggcggat tttagcataa
    17221 tatttcgtgc gctgcttccc tttcgagccg ggagatcatg cacccactaa atgcaggcaa
    17281 acatccgggc ctacatgacg tatctctttg aatttaaatt ggggggcgtc ggctaatttc
    17341 tcaagccctg gcagcgtgca taatccgcgg gcgtcgctgc ctaatagttt aggtgcgata
    17401 tagacaatca gctcatcgac taaacccgcc tgcagcaatg cgccagcgag cgttggcccc
    17461 gcttccaccc agatgctgtt aatttgctgt ttacccagtt gcatcatcag tacaaccaga
    17521 tccagatgac ctttatgctc tggaatcagc aaggtacgca ccgtttccgg ccactcacta
    17581 gaatcttcct gcgtacgcgc gaaccaggtt tcgccgggct gctgcacaat gcgatgttcc
    17641 ggcgtcacgc gattttggct atcaatcaca atacgtactg gctggcggag attttgttgc
    17701 gggtagagcg cctgagtttg ttcatccagt tcagaccaac gcactgttaa ggctggatca
    17761 tccgccagca ccgtggcgct gctggttaaa atggcatgac tttgtgcgcg ctgtcgttgt
    17821 acgtcgcgcc gcgcctgagg cgaagtgatc cactggcttt cgccgctcgc catcgccgtg
    17881 cggccatcaa gcgatgcgcc aagttttaac tgaatataag gaaagccggt gcgcatccgc
    17941 ttgagaaagc ctttattcaa ttgctcggct tcactcatca tcagcccgtg gctgacgtca
    18001 atgccagcct gttgcagacg gtaaagtcca cgcccagcga cctgcgggtt aggatcttgc
    18061 atcgaggcaa ccacgcgcgc tacgccagcg gcgatgagtg cgtcacagca cggtggcgta
    18121 cgaccatgat ggctacaggg ttcgagtgtg acataggcgg tcgcaccttt ggctttttca
    18181 cccgccatac gcaacgcgtg tacttcggca tgtggttcac ccgcacgttg gtggtaacct
    18241 tcaccgacaa tttcgccatc tttgacaatg acgcacccga cattcgggtt gggatgcgtg
    18301 gtaaaacgtc ctcgttgcgc cagctttagc gcccgcgcca tgtaatactc gtcctgcacg
    18361 gcttagtcct ccaggcgcgc gatctcttcg ccaaattctt tgatatcttc gaaactgcgg
    18421 tagacagagg caaaacggat ataggcgact ttatcgagct ttttcaattg ctccatcacc
    18481 agattgccaa tcatcttgct cggcacttcg cgctcaccgg tggcgcgcag ctgcgattta
    18541 atatgattga ttgccatttc gacgtcatcg gaactcaccg gacgtttttc cagcgcccgc
    18601 agcattccgc tacgcaattt ctcttcatta aacggttcac gcacgtcgtt gctttttaca
    18661 acacgcggca taaccagctc cgccacttca aaggtggtga aacgttcatt acacaccaga
    18721 cactgccgac ggcggcgtac ggatgaaccc tcgcccacga gacgagagtc aattacctta
    18781 gtgtccacgg cgaaacagaa tgggcaatgc atacggcgtc ctgaccaggt ggttaacaga
    18841 aatctatttt accctgaact gacgatacaa caaaggcgca acgcttttga gataaaggat
    18901 cgatgctttc gctgctttta ccctctacca ttaggctaat tgcgacgaga aagggacgac
    18961 ctatgaatac taacgttttt cgactgctcc tgctgggaag cctgttcagc cttagcgcct
    19021 gtgtgcagca gagcgaagtg cgacagatga aacacagcgt cagcacgctg aaccaagaga
    19081 tgacgcaact caataaagaa acggtcaaaa tcactcagca aaacaggctg aatgcaaaat
    19141 ccagcagtgg ggtttacctt ctgccaggag cgaaaacacc ggcaagactg gaaagccaga
    19201 tcggtacttt acgtatgtcg ctggtgaata ttacgcctga tgcagatggc accaccctga
    19261 cgctacgtat tcagggcgag tcgaatgatc ccttgcccgc attcagcgga actgttgaat
    19321 atgggcaaat tcagggaaca atagacaact ttcaggaaat caatgtgcag aatcaattga
    19381 ttaatgcccc tgccagcgtc ctcgccccca gcgatgttga tattccgtta cagttaaagg
    19441 gtatttctgt agaacagtta gacttcgtac gtatccacga tattcaacct gttatgcagt
    19501 aaacgtattt cggggcgatt ttgtgcgtct cgcaacatct ttccccgtca ttttgttact
    19561 ccgcttacat caccctgatt gatagtaaaa gtttgcaaca agggtgaaag tcagtacaat
    19621 ccctgcccga atgtgtgtaa acgtgaacgc aatcgattac gtaaatgata gaactgtgaa
    19681 acgaaacata tttttgtgag caatgatttt tataataggc tcctctgtat acgaaatatt
    19741 tagaaacgca atttgcacct ttttcactcc cgcaagggat tttcaaacag tggcatacat
    19801 atgaaaaaaa cattactggc agccggtgcg gtactggcgc tctcttcgtc ttttactgtc
    19861 aacgcagctg aaaacgacaa accgcagtat ctttccgact ggtggcacca gagcgttaac
    19921 gttgtcggaa gctatcacac ccgtttcgga ccgcagatcc gcaacgatac ctaccttgag
    19981 tacgaagcat tcgctaaaaa agactggttc gacttctatg gttatgcgga tgcgccggta
    20041 ttcttcggcg gtaactccga tgctaaaggt atctggaacc acggttctcc gctgtttatg
    20101 gaaatcgaac cacgtttctc catcgacaag ctgaccaata ctgaccttag cttcggtccg
    20161 ttcaaagagt ggtacttcgc gaacaactac atttacgaca tgggtcgtaa taaagatggt
    20221 cgccagagca cctggtacat gggtctgggt accgacatcg atactggtct gccgatgagc
    20281 ctgtccatga acgtctatgc gaaataccag tggcagaact atggcgcagc gaacgaaaac
    20341 gagtgggacg gttaccgttt caaaattaaa tactttgtgc cgattaccga tctgtggggc
    20401 ggtcagctga gctacatcgg cttcaccaac ttcgactggg gttccgattt aggggatgac
    20461 agcggtaacg caatcaacga tattaagacc cgtactaata actctatcgc ttccagccat
    20521 attctggctc tgaactacga tcactggcac tactctgtcg tagctcgtta ctggcacgac
    20581 ggtggtcagt ggaacgacga tgcagaactg aacttcggca acggcaactt caacgttcgc
    20641 tctaccggct ggggtggtta cctggttgta ggttacaact tctgattatg aaaatgccgg
    20701 gatttattcc cggcatttca gattgttaac aacgtgcgct ttgttcatgc cggatgcggc
    20761 gtaaacgctt tgtccgaccc acaaaagcac gtaaattcaa tatattgctg aaataacgta
    20821 gtcctgataa gcgtagcgca tcaggcaatt ttacgtttta atcacttctt cttgttcatc
    20881 atcgctttca ggtcagcgaa tgggttgtac ttcgcttcac cgacatcttt ctgcgcatct
    20941 tcccctgcga taacggtcgt accatactga tccgcttcgg tatatttcga atgctcatga
    21001 tcgtggcaat agagacaaag caattcccag ttactgccat cttccgggtt attggtatgg
    21061 tcgtgatcaa tgtggtgaac agtaagttca cgcaggttgg aataaacaaa ctcgcgggaa
    21121 cagcgaccgc agacccacgg atatattttt aatgcttttt cgcgatagcc gctttctaac
    21181 cgcgcatagt tttttgggat gatagccatg aagagttacc tggatgaaat gtagggttac
    21241 gcgcagtttc ccaagatgcg gggattttcg caatctccat acatcaaaaa aaatcccgat
    21301 cttctgaccg ggattttcaa catcaacggg aacttgatta cggcagaatt gacggctgat
    21361 ccgccccttc tttttccact ttctgctgca acatgtgttc gcgcttcata cccagtttca
    21421 gagccaacgc agatgccaca tagatggaag atgcagtacc gatggaaaca ccgataagca
    21481 tggtcagcga gaagccttcc agtaccggac caccgaagag gtacagcatc aggataacca
    21541 tcaaggtagt accggatgtg atcaaggtac ggtgcagcgt ctgggtcaag gacacgttaa
    21601 agatttcgta aggcgtaccg cgacggatct tgcggaagtt ttcacgaata cggtccgata
    21661 ccacgatact gtcgttaagc gagtaaccga taaccgacat caacgatgcc acaatggtca
    21721 ggtcaatctc gatatggaat aacgacaaaa tacccagcgt aatgataacg tcgtgcgcca
    21781 gcgcaataac aacccctgcc gccagtcgcc actcaaagcg aaaacctacg tacacaagga
    21841 tagacagcag cgccgccatc aacgccatcg caccggtttg cgcaaggtct gcccccacgc
    21901 tcggaccgac gaactcaata cgcttcactg ctgcattctg attggtggat tcgttaatca
    21961 ccttcagaac ctggctgccc agcacctgac cgccggtttc gccttcagca ggcggcatac
    22021 ggaccatgat gtcgtggctg ctaccaaagt tttgcagcat tggctcttca aaaccggctt
    22081 tttgcaatgc atcacgcatt acgtcaattt cagccggttt ttcgagcgta atttcgataa
    22141 ccgtaccacc ggtgaaatcc agcccccagt taaagccgcg cacgcccata ataacgatag
    22201 cggcgattaa cagcagacca gagatgccaa aagcccagta gtcccagcgc ataaagtcat
    22261 agactttacg gccgtggttt agttgttcaa cagtatattc ctgtgccaca tcgcactcct
    22321 cagattgaca gcttcttgac gcgcttgccg ccatatagca ggtttacgat ggcacgcgta
    22381 ccgacaatcg cggtaaacat cgacgtcgcc acaccgatac cggtagtaat cgcgaaccct
    22441 ttaattgccc cggtacccac tgcgtacagg atgatgactt taatcagcgt ggtgatgttc
    22501 gcatcgaaga tagaactgaa tgcgccacga taaccttcat caattgcctg ttgaacagta
    22561 cgtccgttgc tcaactcttc tttaatacgt tcgttgatca gtacgttcgc atcgaccgcc
    22621 accgcaaggg ttaagacgat acccgcgata cctggcatac tcagcgttgc gcctggcaac
    22681 agcgacataa tgccgacgat taagatcaag ttggcaatca gagcactggt cgcaatcaga
    22741 ccaaacttct tatagaagat gatcatgaac agaatagaaa ccagcagacc ggcaaggcaa
    22801 gcctccagcc cctgttcaat gttctgcata cccagggttg ggccaatggt acgttcttca
    22861 acaatctgaa tcggcgcgat caacgcaccg gcacgcagca gcagtgacag ctgacgggct
    22921 tcgttcgggt tgttgatgcc ggtgatacgg aagctgttac ccagacgaga ctggatgttg
    22981 gcgatgttaa tcacctcttc ctgtttcacc agaaccgcac gaccatttgc atctttctta
    23041 ccgctgtctt tgtactccac aaacagggtt gccatcggtt tgccgatgtt gtccttagtg
    23101 aagttagaca tgatgttacc accagcgcta tcgagcgaga tgttaacctg cggctggttg
    23161 tattcgtcct ggctggaagt ggagtcggtg atatggtcac cggtcagaat tacgcgtttg
    23221 tacagcacaa ctggctgacc ttcgcgggtc tgtttcactt cagagtcgcc cggtacgcga
    23281 ccggatgccg cagcggcctg gtcaacgttg gtgtttacca gacggaattc cagcgttgcc
    23341 gtcgcaccca gaatctcttt cgcacgcgca gtgtcctgaa tacctggcag ttcaacaacg
    23401 atacggtcag caccctgacg ctgaaccacc ggctccgcca cgccaagttg gtttacacgg
    23461 ttacgcagga tattgatgtt ctgctgcacc gcatattcac gcgcttcact cagacgagca
    23521 tcgctcatta ccgcacgcag ctggttgctg ccctggctac taatcaccag gtccggatgg
    23581 cgcttgctca gatacgcaat ggcttcatca cgagctttag catcgcggaa agtaatgctc
    23641 aggccgtagt tgttttcttt acgaacggtg gtatacggga tgcccttttc acgcagatcg
    23701 ctgcgcaggc tatcgatatt ttgttcctgg agtttgccaa gcgcggtgtc catatccact
    23761 tccatcagga agtgaacgcc gccacgcagg tcaaggccga gcttcatcgg ctcagcgtga
    23821 atagctgcca gccagcgcgg cgttgccggg gcaaggttaa gcgccacgac gtatttgtca
    23881 cccataacgc ccattaatgc ttcacgagcg cgcagctggg tgtcagtgga gtcgaagcgc
    23941 gcaagaatag cgccctcttc cagtgccaca gacttagcag ttattttttc ttcttgtaac
    24001 gttttctgga cctggatcag cgtttgctca ctggcggcga ctccgcgcgc accagtgatc
    24061 tgaacagccg gatcctcacc aaacaggttg ggaagcgcat acagcagacc gatgacaatc
    24121 accacgatca gcataacgta cttccacaaa ggataacggt ttaacacggc aattccctta
    24181 gggaaaaatt ttaattacag cgccttcatg gtgcctttcg gcaggacggc agctacgaag
    24241 tcacgtttaa taactacttc agtggtgtca ttcagcgcga tagcaatgta gccgttttcc
    24301 gctactttgg ttacgcgacc aaccaggcca ccgttcgtca gaacttcatc acctttggca
    24361 atggagtcca tcagcttttt gtgttctttg gtgcgcttct gctgtggacg caggatcatg
    24421 aaatagaaaa tcagaccgaa taccaccagc atcaaaatca aagacatcgg gctaccttgc
    24481 gccggtgcac ccgttgccgc taccgcatca gaaataaaaa agctcattaa atttccctca
    24541 ttattaatat taatcaacgt tcaaaggtgg tacttctcgc ccctgacgct ggtaaaaatc
    24601 agttacgaag ctctctaatt taccctcttc aatagccttg cgtaaacccg ccatcaaacg
    24661 ctggtagtaa cgaaggttat gaatggtgtt gagtcgcgcg cctaatattt cgttgcaacg
    24721 gtcaagatga tgcaagtaag cgcgtgaata attgcgacag gtgtagcaat cacactcagg
    24781 atcgagtggg ccagtatcgc tcttatactt cgcattgcgg attttcacca cgccatcggt
    24841 cacgaacaaa tgaccatttc gggcgttgcg ggttggcatt acgcagtcaa acatatcgat
    24901 accacgacgt acgccttcaa ccaggtcttc tggtttacca acgcccatca ggtaacgcgg
    24961 tttgtctgcc ggaatttgcg ggcatacatg ctccagaatg cggtgcatat ctgctttcgg
    25021 ctcacccaca gccagaccgc cgacagcgta gccatcaaaa ccgatatcta ccagaccttt
    25081 aacagaaata tcacgtaaat cttcgtaaac gctgccctga atgataccaa acagcgcatt
    25141 tttgtttccg agactgtcaa aacgctcacg gctacgcttc gcccaacgca gagacatctc
    25201 catggagcgt tttgcgtaat cccagtcagc aggatacggc gtacactcat caaagatcat
    25261 gacgatatcc gaaccaagat cgtactgaat ctccattgat ttttcaggat cgaggaaaat
    25321 cggatcgccg ttgatcgggt tacggaagtg cacgccctgt tcggtgattt tacgaatatc
    25381 gccaaggctg aagacctgga agccgccgga gtcggtgagg atcggcccct tccactgcat
    25441 aaaatcgtgc agatcgccgt gcagtttcat gatttcctgg cccgggcgca gccacaggtg
    25501 gaaggtgttg ccgaggataa tttgcgcgcc agtggcttca acttcttccg gcgtcatccc
    25561 ttttacggtg ccgtaggtgc caacaggcat aaaacaaggc gtttccacta cgccacgatc
    25621 aaagaccagg cggccacggc gtgcgcgacc gtcggtggtg tccagttcaa atttcatttt
    25681 ttctccacta cgtcagaaaa acagtccaac gttttaaacc agcgccgcgg aattactccc
    25741 cgacgcgctc attaattgcc tgcggattgt acgtgataaa catcgcatca ccgtaactaa
    25801 aaaagcgata tttctcttct accgctgctt tataggcgtt catggtgtgt tgataaccgg
    25861 caaaggccga aaccagcata atcagcgtcg actctggcaa gtggaagttc gtcaccagcg
    25921 catcgaccac tttgtactgg aagcccggat agataaagat ttgggtatcg tcgaagaacg
    25981 gttcaatgag atcgtttttc gctgcctgag ccgcgctttc cagcgaacgt actgaagtgg
    26041 tgccaaccgc aatcacccgg ttaccgcgcg ctttcgccgc cagtaccgcg tctaccacat
    26101 cctgcggtac ttcagcgtat tccgagtgca tgatgtgatc ttcaatggtg tcgacgcgca
    26161 ccggctggaa ggtgcccgca ccaacgtgca acgtcacaaa cgccatctcc acgcctttgg
    26221 cgcgcaattt ttccagcaaa ggctcgtcaa aatgcagacc agcggtcggg gctgcaaccg
    26281 cgcccggttt ttcgctataa acggtttgat aaagttcgcg gtcagcgtct tcgtccggac
    26341 ggtcgatata cggcggcagc ggcatatggc cgatactgtt gaggatatcc agtaccgaac
    26401 gatcatcatt gaattcgact tcaaacagtg cgccgtggcg cgcggtcatt gttgcgttaa
    26461 tactttcgtc atcgcccagc agcagttctg cgccaggttt tggcgctttc gaggcgcgaa
    26521 tatgcgcaag aatgcgtttg tcgtcgagca tccgttcaac cagcacttca atcttgccgc
    26581 cgctggcttt acgcccaaac aggcgcgccg ggatcacgcg ggtattatta aaaaccagaa
    26641 gatcgccggg gttgagctta tcaagtaaat cggtgaaagt accgtgcgtc agcgcgcccg
    26701 tcggcccgtc cagcgacagt aaacgacagc tactgcgttc aggcatggga tagtgggcaa
    26761 tcagggattc gggcaattca aaggagaaat cggtaacgcg catgacactg actcagacta
    26821 aaataagagg cgggtagtct agtgccgggg cgctctccct gcaaccttta cccccttttg
    26881 gatagaatat aatcgatgaa ttttttagct cacctgcatt tagcccatct cgcggaaagc
    26941 tcgctttccg gcaatttact ggctgatttc gtacgcggaa atcctgaaga aagttttccg
    27001 cccgacgtcg tggctggcat tcatatgcat cgacgtatcg acgtattaac cgacaatctg
    27061 ccggaagtcc gcgaagcacg ggaatggttt cgtagtgaaa ctcgccgcgt tgcgcctatt
    27121 acgctggatg tcatgtggga tcactttctt tcccgccact ggtcgcagct gtcgccggac
    27181 tttccgctac aggaatttgt ctgttatgcc cgcgagcaag tgatgacgat tttgccggac
    27241 tcaccgccac gttttatcaa tctgaacaat tacttgtggt cagagcagtg gctggtgcgc
    27301 tatcgcgata tggatttcat ccagaacgtg ttaaacggca tggcaagccg ccgcccacgt
    27361 ctggatgccc tgcgtgactc ctggtacgat ttagacgctc attatgacgc cctcgaaacc
    27421 cgcttctggc agttttatcc gcggatgatg gcgcaggcgt cacacaaggc gttataaaac
    27481 gttagttcat ccataccgta gccgaaatgg caggcaaagc cagaatcccg tcagtcagtt
    27541 gcccatgccc ttctttgcat tgccattgca cggcattgag caacggtgac gcgggtagca
    27601 cgacttcaca agcttcgcca cggttgattg ccaccagtac acgctgctga ttcagcacgc
    27661 ggacaaatac caccacgtta tcttccgcat acagcacctg acagccgcca tgacgtagcg
    27721 cctgactttt cttacgcagc gcaatcattc gctggtacag cgcgaataac gccgtatcct
    27781 gcttttccac ctgccaaggg aacggtttac ggcaaaacgg atcgtttttg ccatccagtc
    27841 ctacttcatc accgtaataa atgcacggta caccaggcca ggtgaacagc cagaccaccg
    27901 ccagcggcag gcgcgcaata tcccgaccga gcagcgtttt aaatcgcgca gtatcgtggc
    27961 tgtcgagctg attaaacata cgtaattgtt gttgatgaga aagccctgcg cggtaattat
    28021 ccatccaggc catacaggtt tgggcatcaa tttgctgcgg atcgtaagag atatcggtat
    28081 tggcaagaaa tccccacaac gggaatgtga agccacgata gttcatggcg gcatcttcca
    28141 catcggcctg taaccattgc cgtgcatcgc caaaatgttc gccgacaata tacgcttccg
    28201 gctgggtttc tttcgccgct tcggtgatcc cagcaacgtg ctgcatatta tt
//
LOCUS       DBANUU010000056        28185 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000056 DBANUU010000000
VERSION     DBANUU010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.056"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..134)
                     /locus_tag="ACIE68_002337"
     CDS             complement(<1..134)
                     /locus_tag="ACIE68_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFX5908644.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     R"
     gene            complement(205..2319)
                     /gene="fusA"
                     /locus_tag="ACIE68_002338"
     CDS             complement(205..2319)
                     /gene="fusA"
                     /locus_tag="ACIE68_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFX5908645.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="ACIE68_002339"
     CDS             complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="ACIE68_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFX5908646.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="ACIE68_002340"
     CDS             complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="ACIE68_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFX5908647.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="ACIE68_002341"
     CDS             complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="ACIE68_002341"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFX5908648.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3778..4137)
                     /gene="tusC"
                     /locus_tag="ACIE68_002342"
     CDS             complement(3778..4137)
                     /gene="tusC"
                     /locus_tag="ACIE68_002342"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFX5908649.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4137..4523)
                     /gene="tusD"
                     /locus_tag="ACIE68_002343"
     CDS             complement(4137..4523)
                     /gene="tusD"
                     /locus_tag="ACIE68_002343"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFX5908650.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4523..5245)
                     /gene="yheO"
                     /locus_tag="ACIE68_002344"
     CDS             complement(4523..5245)
                     /gene="yheO"
                     /locus_tag="ACIE68_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFX5908651.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5412..6224)
                     /gene="fkpA"
                     /locus_tag="ACIE68_002345"
     CDS             complement(5412..6224)
                     /gene="fkpA"
                     /locus_tag="ACIE68_002345"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFX5908652.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6445..6663
                     /gene="slyX"
                     /locus_tag="ACIE68_002346"
     CDS             6445..6663
                     /gene="slyX"
                     /locus_tag="ACIE68_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFX5908653.1"
                     /translation="MQDLSLETRLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6712..7302)
                     /gene="slyD"
                     /locus_tag="ACIE68_002347"
     CDS             complement(6712..7302)
                     /gene="slyD"
                     /locus_tag="ACIE68_002347"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFX5908654.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7397..7597)
                     /gene="yheV"
                     /locus_tag="ACIE68_002348"
     CDS             complement(7397..7597)
                     /gene="yheV"
                     /locus_tag="ACIE68_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFX5908655.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7607..9412)
                     /gene="kefB"
                     /locus_tag="ACIE68_002349"
     CDS             complement(7607..9412)
                     /gene="kefB"
                     /locus_tag="ACIE68_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFX5908656.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMELQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9412..9963)
                     /gene="kefG"
                     /locus_tag="ACIE68_002350"
     CDS             complement(9412..9963)
                     /gene="kefG"
                     /locus_tag="ACIE68_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFX5908657.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10094..12007
                     /gene="yheS"
                     /locus_tag="ACIE68_002351"
     CDS             10094..12007
                     /gene="yheS"
                     /locus_tag="ACIE68_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFX5908658.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQADEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12007..13029
                     /gene="yheT"
                     /locus_tag="ACIE68_002352"
     CDS             12007..13029
                     /gene="yheT"
                     /locus_tag="ACIE68_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFX5908659.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13023..13241
                     /gene="yheU"
                     /locus_tag="ACIE68_002353"
     CDS             13023..13241
                     /gene="yheU"
                     /locus_tag="ACIE68_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFX5908660.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13295..14164
                     /gene="prkB"
                     /locus_tag="ACIE68_002354"
     CDS             13295..14164
                     /gene="prkB"
                     /locus_tag="ACIE68_002354"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFX5908661.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14219..14623)
                     /gene="yhfA"
                     /locus_tag="ACIE68_002355"
     CDS             complement(14219..14623)
                     /gene="yhfA"
                     /locus_tag="ACIE68_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFX5908662.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14925..15557
                     /gene="crp"
                     /locus_tag="ACIE68_002356"
     CDS             14925..15557
                     /gene="crp"
                     /locus_tag="ACIE68_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFX5908663.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15608..17698
                     /gene="yhfK"
                     /locus_tag="ACIE68_002357"
     CDS             15608..17698
                     /gene="yhfK"
                     /locus_tag="ACIE68_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFX5908664.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFAACSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17765..18985)
                     /gene="argD"
                     /locus_tag="ACIE68_002358"
                     /gene_synonym="dapC"
     CDS             complement(17765..18985)
                     /gene="argD"
                     /locus_tag="ACIE68_002358"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFX5908665.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19071..19634)
                     /gene="pabA"
                     /locus_tag="ACIE68_002359"
     CDS             complement(19071..19634)
                     /gene="pabA"
                     /locus_tag="ACIE68_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFX5908666.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19666..20268)
                     /gene="fic"
                     /locus_tag="ACIE68_002360"
     CDS             complement(19666..20268)
                     /gene="fic"
                     /locus_tag="ACIE68_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFX5908667.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20258..20425)
                     /gene="yhfG"
                     /locus_tag="ACIE68_002361"
     CDS             complement(20258..20425)
                     /gene="yhfG"
                     /locus_tag="ACIE68_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFX5908668.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            complement(20530..21102)
                     /gene="ppiA"
                     /locus_tag="ACIE68_002362"
                     /gene_synonym="rotA"
     CDS             complement(20530..21102)
                     /gene="ppiA"
                     /locus_tag="ACIE68_002362"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFX5908669.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21373..22554
                     /gene="tsgA"
                     /locus_tag="ACIE68_002363"
     CDS             21373..22554
                     /gene="tsgA"
                     /locus_tag="ACIE68_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFX5908670.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWGPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22816..25359
                     /gene="nirB"
                     /locus_tag="ACIE68_002364"
     CDS             22816..25359
                     /gene="nirB"
                     /locus_tag="ACIE68_002364"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFX5908671.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25356..25682
                     /gene="nirD"
                     /locus_tag="ACIE68_002365"
     CDS             25356..25682
                     /gene="nirD"
                     /locus_tag="ACIE68_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFX5908672.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25810..26616
                     /gene="nirC"
                     /locus_tag="ACIE68_002366"
     CDS             25810..26616
                     /gene="nirC"
                     /locus_tag="ACIE68_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFX5908673.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26635..28008
                     /gene="cysG"
                     /locus_tag="ACIE68_002367"
     CDS             26635..28008
                     /gene="cysG"
                     /locus_tag="ACIE68_002367"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFX5908674.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6813 a         7561 c         7125 g         6686 t
ORIGIN      
        1 cgagcagcac cgccgtaggt tttagccagt acggtggtga ttgcagcggt cagagtagtt
       61 ttaccgtggt caacgtggcc gatagtacca acgttaacgt gcggttttgt acgttcaaat
      121 ttttctttag acacggctat attccttact atagtgctct ccccttcagg agagagcacg
      181 ggactttggt attaaccctt aggcttattt accacgggct tcaattacgg cctgagcaac
      241 gttactcggc gcttcatcat acttcaggaa ttccatagtg tatgatgcac gacctttggt
      301 cagagaacgc agctgagttg cgtatccgaa catttcagac agcggtactt cagcgtggat
      361 cttaacgcca gtaacttcag attcctgacc tttgagcata ccacgacgac ggctcaagtc
      421 accgataacg tcaccggtgt tctcttccgg agtttctact tcaaccttca tgatcggctc
      481 aagcagaact ggtttcgctt tcttaaagcc ttctttaaag gcgatagaag cagccagttt
      541 aaacgccagt tcagaggagt caacgtcatg gtaagaaccg aagtgcagac gaatacccat
      601 gtctactacc gggtagcctg ccagcggacc tgctttcagc tgttcctgga tacctttatc
      661 aacggccggg atgtattcgc cagggattac accaccttta atgtcgttga tgaactcgta
      721 gcctttcggg tttgaacccg gctccagcgg gtacatgtcg ataacaacat gaccatactg
      781 accacgacca ccagactgtt tcgcgtgttt accttcaaca tcggtaactt tctggcggat
      841 agtttcacgg taagcaacct gcggtttacc tacgttcgct tcaacgttga attcacgctt
      901 catacggtca acgatgatgt cgaggtgcag ttcgcccata cccgcgatga tggtctggtt
      961 agattcttcg tcagtccata cacggaaaga cgggtcttct ttagccagac ggcccagagc
     1021 cagacccatt ttttcctggt cagctttggt tttcggttca actgcgatgg agattaccgg
     1081 ctcagggaat tccatacgtt ccagaatgat cggcgcatcc gggtcacaca gggtgtcacc
     1141 agtggttacg tctttcagac cgatagcagc agcgatgtcg cccgcgcgaa cttctttgat
     1201 ctcttcacgt ttgttagcgt gcatctgaac gatacgaccg aaacgctcac gtgcagcttt
     1261 cacggagttc agtacggtat caccagagtt aaccacaccg gagtaaacac ggaagaaggt
     1321 caggttacca acaaacgggt cggtagcgat tttgaacgcc agtgcagaga acggctcgtc
     1381 atcacttgcg tgacgttcag ccggagtgtc tttaccgtcg tccaggatac cgttgatcgc
     1441 aggtacgtca accggggatg gcaggtaatc aattaccgca tccagcatcg cctgaacacc
     1501 tttgttcttg aacgcagaac cacaggttac caggatgatt tcgttgttca gaacgcgctg
     1561 acgcagagca cctttgattt ctgcttcagt cagttcttca ccacccaggt atttttccat
     1621 cagctcttca gaagcttcag ctgcggattc gatcaggttc tggtgccatt cgttagccag
     1681 ttcaaccatg tctgccggga tatcttcgta ttcgaaggtt acgccctggt cagcgtcgtt
     1741 ccagttgata gctttcattt tcaccaggtc aacaacaccg gtgaaatgtt cttcagcacc
     1801 aatcgccagc tgcagcggaa ccgggttcgc gcccagacgg gttttgatct ggttaacaac
     1861 tttcaggaag ttcgcaccca tgcggtccat tttgttaacg aacgcaatgc gcggaacttt
     1921 atatttgttt gcctgacgcc atacggtttc agactgcggc tgaacaccac caactgcgca
     1981 gtaaaccatt accgcaccat cgagaacacg catggaacgt tctacttcga ttgtgaagtc
     2041 aacgtgcccc ggggtgtcga tgatgttgat gcgatgcggc tcatactgct tagccatacc
     2101 agaccagaat gcagtagtcg cagcggaagt gatggtaata ccacgttcct gctcctgctc
     2161 catccagtcc atggttgcag cgccgtcatg aacttcaccg attttatggt ttacaccggt
     2221 gtagaacaga atacgttcgg tagtagtggt tttaccggcg tcgatgtgcg cactgatacc
     2281 gatgttacgg tagcgtgcga tgggtgttgt acgagccatt tgtttcctcg tttatctttt
     2341 aggcgttcaa tttaagtagc ccaaagcggg ctgcttactg gaagcgcccg cctggtgact
     2401 aaaactccga agggattacc aacggtagtg tgcgaacgcc ttgttggctt cggccatacg
     2461 gtgaacgtct tcacgtttct taactgcagt acctttgttt tctgcagcat cagaaagttc
     2521 gttcgccagg cgcagagcca tggatttatc accgcgttta cgagcagctt caacgatcca
     2581 acgcattgcc agagcattac gacgaaccgg acggacttca actggtacct gataagtaga
     2641 accaccaacg cggcgagact taacttctac agtcgggcgc acgttttcga gagctacttc
     2701 gaatgcttcc agttcagatt taccagagcg ctgagccagg gtctccagcg cgctgtatac
     2761 gatagattca gcagtagatt ttttaccatc taccatcagg atatttacaa atttagccag
     2821 cagttctgat ccgaacttcg gatccggcag aattttacgc tgaccaatga cgcgacgacg
     2881 tggcatggaa atactccgtt gttaattcag gattgtccaa aactctacga gtttagtttg
     2941 acatttaagt taaaacgttt ggccttactt aacggagaac cattaagcct taggacgctt
     3001 cacgccatac ttggaacgag cctgcttacg gtctttaacg ccggagcagt caagcgcacc
     3061 acgtacggtg tggtaacgaa cacccgggag gtctttaaca cgaccgccac ggatcaggat
     3121 cacggagtgc tcctgcaggt tgtgaccttc accaccgatg taggaagtca cttcgaaacc
     3181 gttagtcaga cgaacacggc atactttacg cagcgcggag ttcggttttt taggagtggt
     3241 agtatataca cgagtacata cgccacgttt ttgcgggcat gcttccagcg caggcacgtt
     3301 gcttttcgca actttgcgag cacgtggttt gcgtaccagc tggttaactg ttgccattaa
     3361 atagctcctg gttttagctt ttgcttcgta aacacgtaat aaaacgtcct cacacaatat
     3421 gaggacgccg aattttaggg cgatgccgaa aaggtgtcaa gaaatataca acgatcccgc
     3481 catcaccagg ccatctggct ggagtgctta accgtaagtc tgacgaaatc agtatagtca
     3541 atgggaatga tgtcgttcga aatttgacca gtcaaaccgc gggcaataag gtcttcgttc
     3601 agggcataga ccttaatggg ggcattacgc agactttcaa ggtagcggtt accgtcaact
     3661 gcggcagtta cgccatcttg caataatagc agttcgtctc cttcactgag cagacgcagc
     3721 agcgcagcaa aatccgtcag ccagggtgaa cgatgtaacg tgtgcagcat aaacgcctca
     3781 aaacctcaaa ataacatcgt agttggcgag ttcgcggcgt aaggcatctg cttcgagcgg
     3841 cgtggcttcg acaacaaagg gtgtctgcgg atctaacccg cgttcgcgca gtgaagccgc
     3901 acaaacccag cactgttcaa tgtcgtacag acccaacaat ttaaaagtgg caatgtaatc
     3961 acgcgccagc actgcatcgg gcttttgtcc tgacagcagc tgaaaaacgc catcagcaat
     4021 aaagaagacg gccagatcgt cagttaatgc ggaagttgcc agtaaagcat ctaaaccttc
     4081 ccgaccagca gctgtaccat gaggtgcagt agaaaaaaca aacgcaattc gtttcatcag
     4141 aactgtacca ccctgtcaca ggtcagcgag gcttccgcca gcgcgccaag tccgcttaag
     4201 gtaaatcccg gctgaaggtt tgacgaagcc agccccagcc ttcctgcttc agtttcatcg
     4261 acgataccgc ggcgtaacgc tgccgctacg cagatattca gcgccacacc atgttgcgca
     4321 ttcaactgtt gccagccgcg cacaaggtca aattcgtcac ttgccggtga ggtcaattgg
     4381 ttagcgttat agaccccttc ccgatagaaa aagacgctgc ttaactcatg gccttctact
     4441 atcagcgcct gcgcaaactg aaaagcacta ctcgcctgtt gcgtaccgta tgctggcccg
     4501 gtcaccacga tggcaaaacg cattacttat cttgcccctg gaaatcaccg cttttgaact
     4561 ggcggatgta gagatagaca gtgtgtttgg agatgttcag gcggtcagca acctggttga
     4621 tcgcgtcttt aatatcgaag atccctttct cgtagagatt cagcacgatc tgacgatttt
     4681 tggcgttatt agaaacattg cgatcggcat ttacttcttc gatggtgaac tccagcgttt
     4741 gggtaaccag atcttcaaca gaagaggcaa agttgacgct tgaaccgaca tccggggttt
     4801 ctggcggcac aaaagtgctc ataatctgcg agaagggaac atcaagattc atattgatgc
     4861 acagcagacc aattacacgc tgttcgcggt tacgaatcgc gatagtcagg gacttcatta
     4921 atacgccgct tttggcgcga gtaaagtagc atttagaaac gctgctatcc gctcccgtca
     4981 tatcgtgcag catacgtagc gccaggtcag taattggcga accaatcttc cggcctgtat
     5041 gttcaccgtt agcaatgcga atggctgaac attttagatc ctgcaaagag tgcaaaacga
     5101 tttcacagtg ggagccaata agcatcgcta acccgtccac caccgcttcg taggatttca
     5161 gaatatcaaa atcggtctgg tcgaaaggac gttgatccag taaatccaac tcactggttt
     5221 cgttggttaa aagcgacctg gacatgaaaa aaaacactcc ttttcaggag cctgtcgtta
     5281 acttttcagg gcaggctcat taatgatgcg ggtaactaaa ttaatacagc ggaggttccg
     5341 ctttccagca ctaattatat ccggcctgta ataaaaaaac cgccgcctgg tcaggcggcg
     5401 gttcttaatg cttatttttt agcagaatct gcggctttcg catcagcttc cggctttgca
     5461 tcagccttcg gcgctggttt cacgtccaac agctctacgt caaacaccag ggtagaattc
     5521 ggtgggatcc ccggaacacc cgctttgccg taagccagtt ctggtggaat aaccagtttg
     5581 atcttaccgc ctttcttgat gttcttcaga ccttctgtcc aacccgggat aacaccgtcc
     5641 agacggaaag agagcggttc accacgagtg taagagttgt cgaactcttt accgtcgatc
     5701 agagtacctt tgtagttcac gacaacagta tcgctgtctt tcggtgcttc gcctttaccg
     5761 gcttctacta cctgataaac cagaccagtt gaagaggttt tcacaccttt ctctttggca
     5821 aatttctcgc ggtactcttt accttttgct tcgttatcag ccgcgtcttt ttccatcttc
     5881 gcctgagcag aagacttcac gcgagcttcg aatgcttgca gagtctgttc gatctcttgg
     5941 tcggagagtt tgctcttatc agcaaatgca tcctgaacac cagcgatcag ctgatcttta
     6001 tccagtttga tgcccagttt ttcttgttct tttagagagt tttccatgta acgacccagc
     6061 gaggcaccca gtgcataagc tgatttctga tcgtcatttt tgaacgctgc tttgctgtca
     6121 gcagttgtag caggttttgc agcttcagca gcaaaagtga ttggtgcatg cagggcaacg
     6181 gccattgtgg tcgccagcag cgttacttta aacagtgatt tcatccatat ctccaggatc
     6241 ggggcatctc accccagggt taactattat cagaagggta ctataaagcg ttgtcgaaca
     6301 aatctacata cagacacgcc ctattatcat ctattttcag actctttttg tttaaattag
     6361 tttcgatgac cgcgaaatga gtgctgtctc gggcagcaaa gttaagtaga atccgcggcg
     6421 accattcgac aaaagaggtg aatcatgcag gatttatcat tggaaacacg cctggcagag
     6481 ctggagagcc gactggcttt tcaggaaatc accattgaag aactgaacgt cacggtgacc
     6541 gctcatgaaa tggagatggc gaaactgcgc gatcatctgc gtctgctgac cgagaagtta
     6601 aaagccagcc agccgtcgaa catcgcgtcg caggctgaag aaacgccacc gccacattat
     6661 tgaggcgtaa aaaaagcggg gattccccgc ttttttgtca ctttttcggt attagtggca
     6721 accgcaaccg ccgttgcctt taccgccaca gcagccttcg ccaccgtgtt catgaccgtg
     6781 atcgtggcca tgaccgccgc agcaaccgtc gtggtcgtga tcgtggtggt gatcgtgcgc
     6841 gccgtgaacg tgaccatgag ccagttcttc ttcagtcgct tcgcgaatcg ccacaacttc
     6901 aacgttgaat ttcaggttct gaccggccag catgtggtta ccatcaacca cgacgtgatc
     6961 gtcttcaacc gcagtgattt caaccggtac cggaccctgg tcggtttcag ccaggaaacg
     7021 catacctacc tgcagttcat caacgcccat aaatacgtct ttaggaacac gttgcaccag
     7081 gttttcgtcg tactgaccgt aagcgtcgtt cgcgccaaca gcgacatcaa atttgtcgcc
     7141 aacttcatga ccttccagcg ccgtttccag gccagagatc agggaaccgt gaccatgcag
     7201 gtagtccagc ggcgcactca ccggagactc atcaaccaac acaccgtctt ctgtacgtac
     7261 ctgataggcc aggctgacca ccaggtcttt tgctactttc atgatatctc ctgagcatgg
     7321 gaagaatagt ggcgcagatt gtagcggaat tctgcagccg tgtactcact agcttaaaaa
     7381 aactcggcgc atatcgctag tccggatgaa aaatcccgat cacttgctca tctttgcgaa
     7441 cgtgatcgcg ggcttctttg tctgcttctc gcatctgatg tccgcactta acacattcaa
     7501 caatatcaat attattttcg cgccacatcg ccattgaatc ctgcgcctga caggccgggc
     7561 attttgcgcc cgcaataaaa cgttttcgga ttgccatctt taccctctac tcaaattcat
     7621 cccagccgtc cagctggcgt cgttcttgtt gcatttcacg ctggaaaatc tcttccagtt
     7681 cgcgtcgggc ttccctggcg cgagaaattt gtacggtatc ggcatgcatt gggatgagct
     7741 ctcgcagcat ccgcatatcc aggcggcgaa aatgcagttg cgctcgctgt gcctgatgcg
     7801 gatgcatgcc aagcgtgacc agcgtcttgc gccccagctc taacgcactg gagaatgttt
     7861 cacgggaaaa ctgcgtcacc cctgcctgta ataactcatg cgcttccaca cgtccgcgcg
     7921 ctcgcgcaag aatatgcaaa tgcggaaagt gctgctggca tatttccacc agcttcatgg
     7981 tgtcttccgg ctcgttacag gtaatgacga tagactcagc ggcctctgca cccgcagaac
     8041 gtaaaagatc gacctgcgtg gcgtcgccgt aataaacttt gtagccgtat ttacgcatca
     8101 ggttaacggc gctgatatcc cgctccagca cggtaatgcg cattttattt gccatcagca
     8161 aacgaccaat cacctgacca aaacgcccga agcccacgac aatgacctgg ggtttatcat
     8221 cgttgaccca cggtttttcg tcttcttctt ccggtccgtt aaactggcgg gatagccatt
     8281 tatccaccag cttcatcagc aacggcgtgg tcatcatgga aagcgtcacc gtcaccagca
     8341 acaacgccat ctggtcgccc tggaataagc gttgtgaaga agcggtagaa aagaggacaa
     8401 aggcaaactc accaccctga ctcaacacgc cagcaaactg catccgctct gagctacgca
     8461 cgccatacaa tcgcgccagc agatacagca cgagaatttt caccgccacc agcacaacca
     8521 cgcttatcac tacccacaac agatgggtat aaagcacccc gaggttgagt gacatgccga
     8581 cagagataaa gaacaaaccg agcagcaagc ctttgaaggg atcgatagcc gtttccagtt
     8641 catggcgata ttcactttcc gccagcagca cgcccgcaat aaatgtaccg agcgccatcg
     8701 acagccccag cgcatccata aacaatgcgg aacccaacac cagcagcagc gtcgcggcgg
     8761 tgaacacttc ccgcacgcca gaagctgcaa taaagcggaa taccggacgc agtaaatagc
     8821 gcccaccaat cagcatgccg acaaacgcca gcaccttcat gccgatcttc atccagtcga
     8881 aatgttcgtc tgccgacccc gccaacaacg gcactaacgc cagtgctggg attactgcca
     8941 gatcctgaaa cagcagaacc gaaaacccga gctggccgga ttcgctgcga ttcatccctt
     9001 tctcacgcat caattgcaat tccattgcag ttgaagacat cgcaaggcca atgccaccga
     9061 ccaccgccgc ctgccaggcg aaatccgtca gcatcaataa tcccgccagc aacgccgcgc
     9121 ttaacagcac ctgtgccgcg cctacgccaa aaatcgaacg ccgcagttgc caaagtttgg
     9181 agggattcaa ctcaaggccg atgataaaca tcaggaatac cacgccgagt tccgaaaagt
     9241 ggaggatctc atcgacgtcg ctaataaacc ccagccccca cgggccaatt gcaatccctg
     9301 ccagcaaata tcccaacaca gcgccaatac ccagccgcga tgccagcggc accgcagcca
     9361 ccgccgcgaa gagaaacagc actcctgcga gtaaaaaatc ggaaccttcc atcagcggcc
     9421 tcctggagac agcggatttg ccagccagtc accgtaggct ctggcgtggc tcgccagctc
     9481 ctgtgcgctt tgccgtctcg cccagtaaat aatgatggga cttaaccaat gcatccggca
     9541 catgcccgcc gccagttcaa aggggcgcag cacatcgctc atcgggtagc gattcagcgc
     9601 gtcataacgg taagcacttt ctggctcgcc ggtggtaatc acgctacgcc agtactttcc
     9661 cgccagttgg tttcctcccg gcccgctggc aaaaccacga cttaataccc ggtccagcca
     9721 ctctttcagt agcgccgggc agctataggt ataaagagga tgctgaaaga caatcacctc
     9781 gtgctcgcgc agtaatgcct gctcacgggg gatatcaata aaaaaatcgg gatagtgcgc
     9841 gtaaaggtcg tgcacggtaa cattgctgag ctgcgtggcc ggtttaagca gtacccggtt
     9901 tgccaccgag tcctgagatt ccggatgggc atacagcagc aaaactttcg ctggctgaga
     9961 catcatcccc ctcccggtat ggtttttgtg tatagtcgct gttttgggct accattgcgc
    10021 ccggtgcggc agctcgccca tacattacat tatcataatg ataagttaac atagtctgaa
    10081 catacggcac cttatgattg ttttctcctc gttacaaatt cgtcgcggcg tgcgcgtcct
    10141 gctggataat gccaccgcca ccatcaaccc agggcagaaa gtcggcctgg tgggtaaaaa
    10201 cggctgtggt aaatctaccc tgctggcatt gctgaaaaat gaaatcagcg ccgacggcgg
    10261 cagctacacc tttccgggaa gctggcaact ggcgtgggtg aatcaggaaa cgccggcgtt
    10321 accgcaagcg gcgctggaat atgtcattga cggcgaccgt gaatatcgtc aactagaagc
    10381 gcagctacac gacgccaacg aacgtaacga cgggcacgcc attgcgacca ttcatggcaa
    10441 gctggatgct attgacgcat ggagtattcg ctcccgtgct gccagcctgc tgcacggcct
    10501 cggtttcagc aatgaacaac tggagcgccc ggtaagtgat ttttccgggg gctggcgtat
    10561 gcgtcttaac cttgcccagg cgctgatttg ccgttcagac ttgctgctgc tcgacgaacc
    10621 gactaaccac ctcgatctcg atgccgttat ctggctggaa aaatggttga agagctatca
    10681 gggcacgctg atcctgatct ctcacgaccg cgacttcctc gatccgatcg ttgataaaat
    10741 tattcatatc gaacaacaaa gcatgttcga gtacaccggt aactacagtt cgtttgaagt
    10801 acagcgcgcc acccgtctgg cgcagcaaca agcgatgtat gaaagccagc aggaacgcgt
    10861 agcgcatctg caaagttata tcgaccgttt ccgtgccaaa gccaccaaag cgaagcaggc
    10921 ccagagccgc attaagatgc tcgagcgtat ggagctgatt gcccccgcgc acgtcgacaa
    10981 cccgttccgc tttagcttcc gcgcgccgga aagcctgcca aatccgttac tgaagatgga
    11041 aaaagtcagc gcgggctatg gcgatcgcat tattctcgac tcgattaaac tgaacctggt
    11101 gcccggctcg cgcattggtc tgttaggccg caacggcgcg ggtaaatcga cattaatcaa
    11161 actgttagcc ggtgaacttg cgccagtcag cggtgaaatt ggtctggcga aagggatcaa
    11221 gctcggctac ttcgcccagc atcaacttga atacctgcgc gccgacgaat cacctattca
    11281 acatctggca cgtttagcgc cgcaggagct ggaacaaaaa ctgcgtgact acctcggcgg
    11341 ctttggtttc cagggcgata aagtaaccga agaaacgcgc cgcttctcag gtggggaaaa
    11401 agcccgcctg gtgctggcat taattgtctg gcagcgtccg aatctgctgc tgctcgacga
    11461 accgactaac caccttgacc tcgacatgcg tcaggcactc accgaagcat taatcgagtt
    11521 cgaaggcgcg ctggttgtcg tttcgcacga ccgtcatttg ctgcgttcca ccactgacga
    11581 tctctacctg gttcacgatc gtaaagtcga accgttcgac ggcgatctgg aagattatca
    11641 acagtggttg agcgacgtac aaaagcagga aaaccaggcc gacgaagcgc caaaagagaa
    11701 cgcgaacagc gcccaggcac gtaaagatca gaagcgtcgg gaagcggagt tgcgtgcgca
    11761 aacccagcca ctgcgtaaag agattgcccg tctggaaaaa gagatggaga agctgaacgc
    11821 gcaactggcg caggcagaag agaaactcgg cgacagcgaa ctgtatgacc agagccgtaa
    11881 agcggagttg accgcctgcc tgcaacagca agccagcgcc aaatccggcc tggaagagtg
    11941 cgaaatggca tggctggaag cccaggagca gcttgagcag atgttgctgg aaggccaaag
    12001 caactgatgg cgcagataac gacgaccgat gccaatgaat tcagcagcag tgctgaattc
    12061 acccctatgc gcggctttag caattgtcat ctgcaaacca tgctgccgcg tctgtttcgt
    12121 cgcaaggtga aattcacccc gtactggcag cggctggagt tgcccgacgg cgattttgtc
    12181 gatctcgcgt ggagtgaagc ccctgcacag gctcggcata aaccgcgttt agtggtattt
    12241 cacgggctgg aaggcagtct caatagccct tacgcccacg gtctggttga ggcggcgcaa
    12301 aagcgcggct ggctgggcgt ggtgatgcat tttcgcggat gcagcggtga accaaaccgt
    12361 atgcaccgca tttaccattc gggcgaaacc gaagacgcca gttggttttt acgctggctg
    12421 caacgcgaat ttggtcatgc gccaacggct gccgtcggct attcgctcgg cggtaatatg
    12481 ctggcctgtt tgctggcaaa ggaaggcaat gatctcccgg ttgatgcggc ggtgattgtc
    12541 tctgcgccgt ttatgctgga agcctgtagc tatcatatgg aaaagggctt ttcccgcgtt
    12601 tatcagcgtt acttgctgaa cctgttaaaa gccaatgccg cgcgcaagct ggcagcctac
    12661 cccggaacgc tgccgattaa tctcgcgcag ttaaaatcgg tacgtcgcat ccgtgaattt
    12721 gacgatctaa tcaccgccag aattcacggc tacgccgacg ctatcgacta ttatcgtcag
    12781 tgtagcgcca tgccgatgct gaaccggatc gccaaaccga cgctgattat tcacgccaaa
    12841 gacgatccgt ttatggatca tcaggtgatc ccgaaaccgg aaagtctccc ctcgcaggtg
    12901 gagtatcaac tgactgaaca tggcggtcat gttggcttta ttggcggtac attacttcat
    12961 ccgcaaatgt ggctggagtc acgcattcct gactggttaa caacgtatct ggaggcgaaa
    13021 tcatgttgat tccgtggcaa gacctctccc ccgaaacgct ggaaaattta attgaaagct
    13081 ttgtgttacg tgaaggcacc gattatggtg aacatgagcg tacacttgaa cagaaagtcg
    13141 ccgacgtcaa acgccagcta cagtgcggag aagcggtgct ggtatggtcg gagctgcacg
    13201 aaacggtcaa tattatgccg cgcagtcagt ttcgcgaata acctgcaacc gtcggcggac
    13261 gagataataa tgatagctaa gtcatggagt tactatgtct gccaaacatc cggtcattgc
    13321 ggtaacagga tccagcggcg cggggaccac caccaccagc ctcgcgtttc gtaaaatatt
    13381 cgcgcagtta aatctgcatg cagctgaggt ggaaggcgac agttttcacc gttacacccg
    13441 cccggaaatg gacatggcga tccgcaaagc gcgcgacgcc gggcggcata tcagctactt
    13501 cggccccgaa gcgaacgact tcggcctgct ggaacaaacc ttcattgaat acggtcagag
    13561 cggcaaaggg aaatctcgca aatatctgca tacctacgac gaagccgtac cctggaatca
    13621 ggtaccggga acattcaccc cctggcaacc tttaccagaa cccactgatg tactgtttta
    13681 tgaaggttta cacggcggcg tagtcacgcc acagcataac gttgcgcagc atgtggactt
    13741 actggtcggc gtagtgccta tcgttaacct tgagtggatt caaaaactga tccgcgacac
    13801 cagcgagcgc gggcactcac gagaagcagt gatggactcg gtagtgcgtt caatggaaga
    13861 ctatatcaac tacatcacac cgcagttttc ccgcacccat cttaacttcc agcgcgttcc
    13921 caccgtcgac acttcaaacc cgttcgcggc aaaaggtatc ccgtcgctgg atgaaagctt
    13981 tgtggtgatc cattttcgta atctggaagg gatcgatttc ccctggctgc tggcgatgtt
    14041 gcaaggctca ttcatttccc acatcaatac gttagtggtg cccggcggca aaatgggtct
    14101 ggcaatggaa ttaattatgc tgccgctggt gcaacgattg atggaaggaa agaaaatcga
    14161 gtaactctgc tattacgccg gataaaatac tatccggctt cacaacggga tagttaaatc
    14221 acgcggcaac cacttcatac gagtgagtaa tattcaccgc tttttccagc atcaacgcca
    14281 ctgaacaata tttctcagca gagagatcaa ccgcacgcgc aaccgctgcg tctttcaggt
    14341 cgcgaccggt gacgataaaa tgcagattaa tgtgcgtaaa caggcgtggt gcctcttcgc
    14401 ggcgttcaga ggtcaatttt acttcacaat cgaccacatc ctgacgccct ttttgcagga
    14461 tcgaaaccac atcgatggca ctgcaaccac ccgccgccat caacaccatt tccatcggac
    14521 ttggtgcttt atcgcctgag ttgccgtcca ttaaaatctg atgaccagag gcggattcgc
    14581 ccagaaaagt taacccttcg acccacttca ctcgcgcttg catttttgct actccactgc
    14641 gtcaattttc ctgacagagt acgcgtacta accaaatcgc gcaacggaag gcgacctggg
    14701 tcatgctgaa gcgagacacc aggagacaca aagcgaaagc tatgctaaaa cagtcaggat
    14761 gctacagtaa tacattgatg tactgcatgt atgcaaagga cgtcacatta ccgtgcagta
    14821 cagttgatag ccccttccca ggtagcggga agcatatttc ggcaatccag agacagcggc
    14881 gttatctggc tctggagaaa gcttataaca gaggataacc gcgcatggtg cttggcaaac
    14941 cgcaaacaga cccgactctc gaatggttct tgtctcattg ccacattcat aagtacccat
    15001 ccaagagcac gcttattcac cagggtgaaa aagcggaaac gctgtactac atcgttaaag
    15061 gctctgtggc agtgctgatc aaagacgaag agggtaaaga aatgatcctc tcctatctga
    15121 atcagggtga ttttattggc gaactgggcc tgtttgaaga gggccaggaa cgtagcgcat
    15181 gggtacgtgc gaaaaccgcc tgtgaagtgg ctgaaatttc gtacaaaaaa tttcgccaat
    15241 tgattcaggt aaacccggac attctgatgc gtctgtctgc acagatggcg cgtcgtctgc
    15301 aagtcacttc agagaaagtg ggcaacctgg cgttcctcga cgtgacgggc cgcattgcac
    15361 agactctgct gaacctggca aaacaaccag atgctatgac tcacccggac ggtatgcaaa
    15421 tcaaaattac ccgtcaggaa atcggtcaga ttgtcggctg ttctcgtgaa accgtgggac
    15481 gcattctgaa gatgctggaa gatcagaacc tgatctccgc acacggtaaa accatcgtcg
    15541 tttacggcac tcgttaatcc cgtcggagtg gcgcgttact gagtagcgcg ccattttgtt
    15601 tcccccgatg tggcgcagac tgatttatca ccccgatatt aactatgcac tgcggcaaac
    15661 gctggtgctc tgtttgcccg tggccgttgg gttaatgctt ggcgaattac gattcggtct
    15721 gctcttctcc ctcgttcctg cctgttgcaa tattgcgggc cttgatacgc ctcataaacg
    15781 ttttttcaaa cgcttaatca ttggtgcgtc gctgtttgca gcctgtagct tgctgacaca
    15841 gctgctactg gcaaaagatg ttcccctgcc ctttttgctg accggattaa cgctggtact
    15901 tggcgtcact gctgagttgg ggccattgca cgcaaaattg cttcccgcat cgctgctggc
    15961 agccattttt accctcagtc tggcgggata catgccggtc tgggaaccgt tgctcatcta
    16021 tgcgttgggc actctctggt acggattgtt taactggttt tggttctgga tctggcgcga
    16081 acaaccgctg cgcgagtcac taagtctgct gtaccgtgaa ctagcagatt attgtgaagc
    16141 caaatacagc ctgcttaccc agcacaccga ccctgaaaaa gcgctgccgc cgctgctggt
    16201 gcgccagcaa aaagcggtcg atctaattac ccagtgctat cagcaaatgc atatgctttc
    16261 cgcgcaaaat aatactgact acaagcggat gctgcgtatt ttccaggagg cgctggattt
    16321 acaggaacat atttcggtca gtttgcatca gccggaagag gtgcaaaagc tggtcgagcg
    16381 tagccatgcg gaagaagtta tccgctggaa tgcgcaaacc gtcgccgctc gcctgcgcgt
    16441 gctggctgat gacattcttt accatcgcct gccaacgcgt tttacgatgg aaaagcaaat
    16501 tggcgcactg gaaaaaatcg cccgccagca tccggataat ccggttgggc aattctgcta
    16561 ctggcatttc agccgcatcg cccgcgtgct gcgcacccaa aaaccgctct atgcccgtga
    16621 cttactggcc gataaacagc ggcgaatgcc gttacttccg gcgctgaaaa gttatctgtc
    16681 actaaagtct ccggcgctac gcaatgccgg acgactcagt gtgatgttaa gcgttgccag
    16741 cctgatgggc accgcgctgc atctgccgaa gtcgtactgg atcctgatga cggtattgct
    16801 ggtgacacaa aatggctatg gcgcaacccg tctgaggatt gtgaatcgct ccgtgggaac
    16861 cgtggtcggg ttaatcattg cgggcgtggc gctgcacttt aaaattcccg aaggttacac
    16921 cctgacgttg atgctgatta ccaccctcgc cagctacctg atattgcgca aaaactacgg
    16981 ctgggcgacg gtcggtttta ctattaccgc agtgtatacc ctgcaactat tgtggttgaa
    17041 cggcgagcaa tacatccttc cgcgtcttat cgataccatt attggttgtt taattgcttt
    17101 cggcggtact gtctggctgt ggccgcagtg gcagagcggg ttattgcgta aaaacgccca
    17161 tgacgcttta gaagcctatc aggaagcgat tcgcttgatt cttagcgagg atccgcaacc
    17221 tacgccactg gcctggcagc gaatgcgggt aaatcaggca cataacactc tgtataactc
    17281 attgaatcag gcgatgcagg aaccggcgtt taacagccat tatctggcag atatgaaact
    17341 gtgggtaacg cacagccagt ttattgttga gcatattaat gccatgacca cgctggcgcg
    17401 ggaacaccgg gcattgccac ctgaactggc acaagagtat ttacagtctt gtgaaatcgc
    17461 cattcagcgt tgtcagcagc gactggagta tgacgaaccg ggtagttctg gcgatgccaa
    17521 tatcatggat gcgccggaga tgcagccgca cgaaggcgcg gcaggtacgc tggagcagca
    17581 tttacagcgg gttattggtc atctgaacac catgcacacc atttcgtcga tggcatggcg
    17641 tcagcgaccg catcacggga tttggctgag tcgcaagttg cgggattcga aggcgtaatg
    17701 caggctaaac catcggataa ggcattcacg ccgcatccga catcttttgc ctgatgcttc
    17761 gtatttacgc cccaaccacc ttcgccaccg cgtgggcgaa acgttgcatc ccttcatcga
    17821 tatccgcatc ttccaccacc agcgacggtg caaaacgcat cacatccggt ccggcattca
    17881 gcaccattac gccagcctct gcgcccgcat acaggaaatc acgcgcccga cctttgtact
    17941 gtggtttcag ctctgcgcca atcaacagcc ccataccgcg aatatcgcta aacacatcgt
    18001 actgctgatc gatcttctgc agatggtcaa caaaacgctg gcgtttcgcc tgaatgcctt
    18061 ccagcacttc aggggtattg atgatatcaa acgccgcccc cgctactgca caggccagag
    18121 gattaccgcc gtaggtggaa ccgtgagaac caggatgaaa cgcagaagca atttccgccg
    18181 tggtcagcat ggcgctaatc gggaagccgc cgcctaacgc tttcgcagag gtcagaatat
    18241 ccggcgtaac gccgtagtgc atgtaagcaa acaaatcgcc ggtccgcccc atcccgcact
    18301 gcacttcatc aaacaccaat aatgcctgat gttgatcgca cagctcgcgc aagccctgca
    18361 aaaactctgg cgtcgctgcc gtcacaccgc cctcgccctg gatcggctca accaccaccg
    18421 cacaggtgtg atcatccatc accgctttca ctgcgtggag atcgttaaaa ggaacgtgga
    18481 tgatgtctgc cggttttggc ccaaagccgt cggaatattt tggctgcccg cccaccgaga
    18541 cggtaaacag cgagcgacca tgaaaagcgt tatggaaggc aataattttg gttttgaacg
    18601 gactatgacg cacacaggcg taatggcgtg ccagtttaaa ggcggtttcg ttagcttccg
    18661 tgccggagtt cataaacacc acgcgttcgg caaacgtcgc ctcaatcagt ttgcgcccaa
    18721 gacgcagcgc cggttcattg gtgaaaacgt tactgatatg ccacagagtt tcgccctggg
    18781 tttttaacgc gttcaccagc gcaggatggc aatggcccaa cgccgtaact gcaatgccac
    18841 ccgcgaaatc gacatactcc ttgccttgct gatcccagat tcggctgccc tgacctttta
    18901 ccggaatgaa ctctgccgga gcataaatcg gcaggatcac ttcatcgaaa gtcgcgcgtg
    18961 taattgctgt ttgttcaatt gccatctcat gatcaccctg ttacgcataa ataaatgtga
    19021 aattataatc acaaaatatg cataaaaaat cattgaatgg caatcagaaa tcagcgatgc
    19081 aggaaattag ccagcagttg atgtccttgt tcgctaagaa tactttctgg atggaactgc
    19141 acaccttcca gatcccactg gcgatggcga atccccataa tctcgcgggt ttcgctccag
    19201 gccgtcacgt caaagcatgc tggtaatgag ccaggttcca ccaccagcga atggtagcgt
    19261 gtcacggtaa gtggatttgc cagcccccga aatacgccct caccgttatg tgtaatcggc
    19321 gaggttttgc cgtgcatgac ctttgcggcg cgcacaactt tgccgccaaa tgcctgcgcc
    19381 attgcctgat gaccgaggca gacgccaaga atcggcaagc gcccggcata gtggcgaata
    19441 acgtcaaggg agatcccggc ttcatctggc gtacaggggc cgggtgatat gacaattttt
    19501 tgtggtttaa gggcgtcgat atccgccagc gtcaacgcat cgttgcgctt aaccagcaca
    19561 tccgccccca gttcacaaaa gtactggtag aggttccagg taaaagaatc gtagttatct
    19621 ataagcagga tcatggcggc tccggtacga aagaaccgcg ctattttact cagattcccc
    19681 ggcttcgctt accactttgc taaatatcat ctgcagcgcg gtgagatccc ccattgcccc
    19741 actctgattt gcctgattcc aggcctcttt ttcgataccc tgccagctca gttgataccc
    19801 ggcatgaatc gccagttgct cgaagaagat ccgctgtgcc agaccacttc ccacccggaa
    19861 gggatgcagc acgttgattt cacaatagta atgcgccagc cgctcgacga acttcgcttt
    19921 ctccaggcca accagataac cttcttcctc cagatcctgc atcagggcat tgccctcttt
    19981 ttcgatataa gcaaagtggc agaacggcgt atcgccctga taaatatcaa cttcacgcag
    20041 ttgccctgcc cagtcgaaaa tatcctgata cagctggcga tggatagtgc gcaaatgcgg
    20101 taaaccgcgc accagcggac caagctcaat ggtcgcagca cgcagcgccg tcatttcgta
    20161 agcggcctgt tccagccgtt gttgctggcg gatgttcagc cggttacgca tgatatcaag
    20221 gccaggataa agatacggat cgcgcccttc gccgaattta tcgctcatag tgactcctca
    20281 gctcttcaag gcgcgctaaa gcctctgcgg cagtaagagt gactaaaggc atctcgacgc
    20341 cttcaagacg gcgactagcc tgaaaattac gattacgctg aagctcccag agacgggact
    20401 tttgcttatc ggtgagtttc ttcacttgat gcctccctga acgtgtccat ttgccacaag
    20461 tataagcggc aaatccgggt tacgccggga gagcagaagc gcgggcaaga ttgcccgcgc
    20521 gagaaatcat tacggcagga ctttagcgga aaggataact accggttttg acggcacatt
    20581 ctggtacgga ccaacgtcat gagtcggcac ctgggaaatc ttatcggcca cgtccatgcc
    20641 tttcaccact ttaccaaata ccgcgtaacc gaaatcacgc tgaccatggt caaggaaggc
    20701 gttatcggca acgttgataa agaactggct ggtggcgctg tctttgtcag cggtacgcgc
    20761 catcgcgatg gtaccacgcg tgttgcgcag gccgttatcg gcttcatttt tgattggcgg
    20821 gtttggtttt ttctgctgca tctgctcggt gaaaccgccg ccctgaatca taaagccagg
    20881 aatgacgcgg tgaaaggtag tgttgttata aaaaccgctg ttcacataat cgacaaagtt
    20941 ttgcacagac actggcgctt tttgtttatc cagctccagt tcgatgttac cagctgaggt
    21001 tgtcaacaat acgtgcgggt cccctttcgc tgccattgct gcgggagaaa gagcagaaag
    21061 agcgaaaaca gcagccatcg ccgccagggt cgatttgaac atgagatttc cttaacaaac
    21121 agagcagaaa aaaagcaagt gaagagattc taaatagcct tcaagaacaa ggccatccct
    21181 ttacctaatt ttacgtatct gaaatatatg taacctgacg atcacttttt attccgtgat
    21241 caaaatcacc tcttaaaatg caatttagca accgattgca ataaaacatt taaacagatc
    21301 acaaaattac ctaaaatcgc ccgctcgcgg catatcccgc tatgccattt tatttttgaa
    21361 cgcaggccaa tcatgactaa cagcaatcgc atcaagctca catggattag ctttctctcc
    21421 tacgcactga ctggtgcgtt ggttattgtc accgggatgg tgatgggaaa tatcgccgat
    21481 tatttcaatc tgcctgtttc cagtatgagt aacaccttca ccttcctcaa cgccggcatt
    21541 ttaatctcta tcttcctcaa cgcctggctg atggaaatcg tcccgttgaa aacgcagtta
    21601 cgttttggct ttctcctgat ggtgctggcg gttgccggtt tgatgttcag ccacagcctg
    21661 gcgctgttct cggcggcgat gttcattctc ggggtggtca gcggcatcac catgtcgatt
    21721 ggtacattcc tggtaacaca aatgtatgaa gggcgtcagc gcggttcccg cctgttattt
    21781 accgactcct tcttcagtat ggctgggatg attttcccaa tgatcgccgc gtttctactg
    21841 gcgcgcagca ttgagtggta ctgggtttat gcctgcatcg ggctggtgta tgtcgctatt
    21901 tttattctga ccttcggctg tgagttcccg gcgctgggca aacatgcgcc aaaaacggat
    21961 gctccggtag agaaagaaaa gtgggggatc ggcgtactgt ttctctctgt tgcggcgctg
    22021 tgctacatcc tcggtcagtt aggttttatc tcctgggggc ctgagtatgc caaaggcctg
    22081 ggcatgagcc tgaacgacgc gggcacgctg gtgagtaact tctggatgtc atacatggtc
    22141 ggcatgtggg cgttcagctt tattcttcgc ttctttgatt tgcaacgcat tctgaccgta
    22201 ctggctggtc tggctgcgat tctgatgtac gtctttaaca ccggaacacc agcacatatg
    22261 gcgtggtcaa ttctcgctct gggcttcttc tccagcgcga tctataccac catcatcacc
    22321 ctgggttcac agcagaccaa agtaccgtcg ccaaaactgg ttaactttgt cctgacctgc
    22381 ggaaccatcg gtactatgtt gacctttgtg gttaccggcc cgattgttga acatagcggt
    22441 ccgcaggcgg cactgcttac agcaaacggt ctgtacgctg tcgtctttgt gatgtgcttc
    22501 ctgttaggtt tcgtcagccg tcaccgtcag cataacaccc tgacctctca ttaattgcta
    22561 atgccggacg gcactatcgt cgtccggcct tttcctctct tcccccgcta cgtgcatcta
    22621 tttctataaa cccgttcatt ttgtctattt tttgcacaaa catgaaatat cagacaattc
    22681 cgtgacttaa gaaaatttat acaaatcagc aatatacccc ttaaggagta tataaaggtg
    22741 aatttgattt acatcaataa gcggggttgc tgaatcgtta aggtaggcgg taatagaaaa
    22801 gaaatcgagg caaaaatgag caaagtcaga ctcgcaatta tcggtaacgg tatggtcggc
    22861 catcgcttta tcgaagatct tcttgataaa tctgatgcgg ccaactttga tattaccgtt
    22921 ttctgtgaag aaccgcgcat cgcttatgac cgcgtacacc tctcgtctta cttctctcac
    22981 cacaccgccg aagagctgtc gctggtgcgc gaaggcttct acgagaaaca cggcatcaaa
    23041 gttctggtcg gcgaacgcgc tatcaccatc aaccgtcagg agaaggtgat tcactccagc
    23101 gccggacgta ccgtttttta tgacaagctg atcatggcaa ccggttccta cccgtggatc
    23161 ccaccaatca aaggttctga tactcaggac tgctttgtct atcgcaccat tgaagacctc
    23221 aacgccattg aatcctgcgc ccgtcgcagc aaacgcggtg ccgttgttgg tggcggcctg
    23281 ttaggtctgg aagccgctgg cgcgctgaaa aacttaggta ttgaaaccca cgttatcgaa
    23341 tttgccccta tgctgatggc ggaacagctt gatcagatgg gtggcgagca gctgcgtcgc
    23401 aaaatcgaaa gtatgggcgt gcgcgttcac accagcaaaa acacccttga gattgtgcag
    23461 gaaggtgtcg aagcgcgtaa aaccatgcgt tttgccgacg gcagcgaact ggaagtcgac
    23521 tttatcgtct tctctaccgg tatccgtccg cgcgataagc tggcaaccca gtgtggtctg
    23581 gacgttgctc cgcgtggggg tattgtcatt aatgattcct gccagacttc cgatccggat
    23641 atctacgcca tcggtgaatg cgcaagctgg aacaaccgtg tatttggtct ggtagcacca
    23701 ggctacaaaa tggcgcaggt cgccgttgac catattctcg gtagcgaaaa cgcctttgaa
    23761 ggtgctgacc ttagcgccaa gctgaaactg ctgggcgtag acgtaggcgg tattggtgat
    23821 gcgcacggtc gcacgcctgg cgcacgtagc tacgtttacc tcgacgaaag caaagagatc
    23881 tacaaacgcc tgattgtcag cgaagacaac aaaaccctgc tcggtgcggt actggtgggc
    23941 gataccagcg actacggcaa cctgctgcaa ctggtgctga atgctatcga gctgccggaa
    24001 aacccggatt ctctgattct gccggcgcac tcgggtagcg gtaagccgtc tatcggtgtt
    24061 gataaactgc cggacagcgc gcaaatctgc tcctgcttcg acgtcaccaa aggtgatctg
    24121 attgctgcca tcaacaaagg ctgccacaca gttgcggcgc tgaaagctga aaccaaagcg
    24181 ggtactggct gcggtggctg tatcccgctg gtcactcagg tactgaacgc ggaactggcg
    24241 aaacagggca tcgaagttaa caacaacctg tgcgaacact ttgcttattc gcgtcaggaa
    24301 ctgttccatt tgatccgcgt tgaaggcatt aaaaccttcg aagaactgct ggcgaaacac
    24361 ggcaaaggct acggttgtga agtttgtaaa ccaaccgtcg gttcgctgct ggcctcctgc
    24421 tggaacgaat acattctgaa gccggaacat actccgctgc aggattctaa cgacaacttc
    24481 ctcgctaaca tccagaaaga cggcacctac tcggtgatcc cgcgttctcc gggcggtgaa
    24541 atcaccccgg aagggctgat ggcagtgggt cgtatcgcgc gtgaatttaa tctctacacc
    24601 aaaatcaccg gctcccagcg cctggcgatg tttggcgcac agaaagacga tctgccggaa
    24661 atctggcgtc agctgattga agctggcttc gaaaccggtc atgcctatgc gaaagcactg
    24721 cgtatggcga aaacctgcgt gggtagcacc tggtgccgct acggtgttgg cgatagcgtc
    24781 ggcctcggcg tggaactgga aaaccgctac aaaggcatcc gtacgccgca caaaatgaag
    24841 ttcggtgtct ccggctgtac ccgtgaatgt tcagaagctc agggtaaaga cgtgggtatt
    24901 atcgccactg aaaaaggctg gaacctgtat gtttgcggta acggcggcat gaaaccgcgt
    24961 catgcggatc tgctggcagc ggatatcgat cgcgaaacgc tgatcaaata tctcgaccgc
    25021 ttcatgatgt tctacatccg taccgccgac aaactgacgc gtaccgcgcc gtggttagaa
    25081 aacctcgaag gcggcatcga ttacctgaaa gcggtgatca ttgacgacaa actggggctg
    25141 aacgctcatc tggaagaaga gatggcgcgc ctgcgtgaag cggtagtgtg tgagtggact
    25201 gaaacggtca atacaccgtc cgcgcagact cgcttcaaac acttcatcaa cagcgacaag
    25261 cgtgacccga acgtgcagat ggtgccagag cgcgaacagc accgtccggc aacgccgtat
    25321 gaacgtatcc cggtaactct ggtggaggac aacgcatgag ccagtggaaa gacatctgca
    25381 aaatcgatga catcctgcct gaaaccggcg tctgcgcgct gttaggcgac gagcaggtcg
    25441 cgattttccg cccgtatcac agcgaccagg tgtttgcgat cagcaacatc gatccgttct
    25501 tcgagtccag cgtgctgtca cgcgggctga ttgcggaaca ccagggcgag ctgtgggtcg
    25561 ccagcccgct gaaaaaacag cgttttcgct taagtgatgg cttgtgcatg gaagacgaac
    25621 agttttccgt caaacattac gaagcacgag tgaaagacgg cgtggtgcag ctgcgcggtt
    25681 aatgttttaa cgggaggcgc aatgcctccc ctttttgcat ggtcctgtaa tgaccttcgg
    25741 tatattgcag gacatttttt aaactttttg ttttattttt tttgttttta ttttttaaag
    25801 gataatcaaa tgtttacaga cactattaat aagtgtgcgg ctaacgctgc gcgcattgca
    25861 cgcctgtcgg caaataaccc gctcggcttt tgggtcagct ccgccatggc gggcgcgtat
    25921 gtgggtcttg ggatcatctt gattttcacg ctcggtaatt tgctcgaccc gtccgtacgt
    25981 ccgctggtga tgggcgcgac ctttggtatc gccttaacgc tggtgattat cgccggttct
    26041 gaactgttca ccggacacac catgttcctc acctttgggg taaaagcggg cagcatcagc
    26101 cacgggcaaa tgtgggcaat cctgccgcaa acctggctgg gtaacctggt cggttccgtc
    26161 ttcgttgcca tgctctatag ctggggcggc ggtagcctgc tgccggtaga taccagcatc
    26221 gttcactccg tcgcgctggc taaaaccact gcaccggcaa tggtactgtt cttcaaaggt
    26281 gcattgtgta actggctggt ttgcctggca atctggatgg cgctgcgcac tgaaggggcg
    26341 gcgaaattta tcgctatctg gtggtgtctg ctggcattta tcgcgtccgg ctacgagcac
    26401 tctatcgcta acatgacgct gttcgcgctc tcctggttcg gcaaccacag cgaagcctac
    26461 acgctggcgg gtattggtca taacctgctg tgggtgacgc tgggtaatac tttatcaggt
    26521 gccgtattca tgggattggg ttattggtat gctacgccga aagcgaatcg tccggttgcg
    26581 gacaaattta atcaaactga aacggctgcc ggttaattac taaggggttt ttacgtggat
    26641 catttgccta tattttgcca attacgcgat cgcgactgtc tgattgtcgg cggtggtgat
    26701 gtcgcggaac gcaaagcaag gttgctgtta gacgcaggcg ctcgcttaac ggtgaatgca
    26761 ttagcgttta ttccacagtt caccgcatgg gcagatgcag gcatgttaac cctcgtcgaa
    26821 gggccatttg atgaaagcct tctcgacacc tgctggctgg ctattgcagc gacggatgat
    26881 gacgcgctta accagcgcgt cagcgaagcc gctgaagctc gtcgcatctt ctgtaacgtg
    26941 gtcgatgcgc cgaaagccgc cagctttatt atgccgtcga ttattgaccg ctcaccgctc
    27001 atggtagcgg tctcctctgg cggcacctct ccggttctgg cacgcctgtt gcgcgaaaaa
    27061 cttgaatcac tgctgccgtt acatctgggc caggtagcga aatacgccgg gcaattacgc
    27121 gggcgagtga aacaacagtt cgccacgatg ggtgagcgtc gccgtttctg ggagaaattg
    27181 ttcgttaacg accgcctggc gcagtcgctg gcaaacaacg atcagaaagc cattactgaa
    27241 acgaccgaac agttaatcaa cgaaccgctc gaccatcgcg gtgaagtggt gctggttggt
    27301 gcaggtccgg gcgatgccgg gctgctgaca ctgaaaggac tgcaacaaat tcagcaggca
    27361 gatgtggtgg tctacgaccg tctggtttct gacgatatta tgaatctggt acgccgcgat
    27421 gcggaccgtg ttttcgtcgg caaacgcgcg ggataccact gcgtacccca ggaagagatt
    27481 aaccagatcc tgctgcggga agcgcaaaaa ggcaaacgcg tggtgcggct gaaaggtggc
    27541 gatccgttta tttttggccg tggtggcgaa gagctggaaa cactgtgcaa cgcgggtatt
    27601 ccgttctcgg tggttccggg tattaccgca gcttctggtt gctctgccta ttcgggtatt
    27661 ccactcacgc atcgcgatta tgcccagagc gtacgcttaa ttaccggaca cttaaaaacc
    27721 ggtggcgagc tggactggga aaacctggcg gcagaaaaac agacgctggt gttctatatg
    27781 gggttgaatc aggccgcgac tattcagcaa aagctgattg aacacggtat gcctggcgaa
    27841 atgccggtgg caattgtcga aaacggaacg gcagtcacgc agcgcgtgat tgacggtacg
    27901 ctcacgcaac tcggtgaact tgctcagcaa atgaacagtc catcgctaat tattattggt
    27961 cgggttgttg gcctgcgcga taaactgaac tggttctcca accattaatt taacccgggc
    28021 cagagaattc tggccttctt aatgactctt tttatatatt cagcaaataa aacatagccc
    28081 cttaataaat attatctgct aatgggttat attcaccgtt acagcgactt acaaaattaa
    28141 gtcattcgct cactgacgcc aattaaatat tcctgtatcg ttctt
//
LOCUS       DBANUU010000057        26637 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000057 DBANUU010000000
VERSION     DBANUU010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.057"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            236..1885
                     /locus_tag="ACIE68_002368"
     CDS             236..1885
                     /locus_tag="ACIE68_002368"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFX5908675.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(1936..2538)
                     /locus_tag="ACIE68_002369"
     CDS             complement(1936..2538)
                     /locus_tag="ACIE68_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFX5908676.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            3058..3957
                     /locus_tag="ACIE68_002370"
     CDS             3058..3957
                     /locus_tag="ACIE68_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFX5908677.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            4006..5019
                     /gene="yhjD"
                     /locus_tag="ACIE68_002371"
     CDS             4006..5019
                     /gene="yhjD"
                     /locus_tag="ACIE68_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFX5908678.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            5430..6752
                     /locus_tag="ACIE68_002372"
     CDS             5430..6752
                     /locus_tag="ACIE68_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5908679.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(6986..9046)
                     /locus_tag="ACIE68_002373"
     CDS             complement(6986..9046)
                     /locus_tag="ACIE68_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFX5908680.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(9116..9883)
                     /gene="pdeH"
                     /locus_tag="ACIE68_002374"
     CDS             complement(9116..9883)
                     /gene="pdeH"
                     /locus_tag="ACIE68_002374"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFX5908681.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVAHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            10115..11044
                     /gene="kdgK"
                     /locus_tag="ACIE68_002375"
     CDS             10115..11044
                     /gene="kdgK"
                     /locus_tag="ACIE68_002375"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFX5908682.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(11140..12636)
                     /locus_tag="ACIE68_002376"
     CDS             complement(11140..12636)
                     /locus_tag="ACIE68_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFX5908683.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(12857..14143)
                     /gene="dctA"
                     /locus_tag="ACIE68_002377"
     CDS             complement(12857..14143)
                     /gene="dctA"
                     /locus_tag="ACIE68_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFX5908684.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(14326..16314)
                     /gene="hmsP"
                     /locus_tag="ACIE68_002378"
     CDS             complement(14326..16314)
                     /gene="hmsP"
                     /locus_tag="ACIE68_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFX5908685.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(16396..19869)
                     /gene="bcsC"
                     /locus_tag="ACIE68_002379"
     CDS             complement(16396..19869)
                     /gene="bcsC"
                     /locus_tag="ACIE68_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFX5908686.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(19851..20957)
                     /gene="bcsZ"
                     /locus_tag="ACIE68_002380"
     CDS             complement(19851..20957)
                     /gene="bcsZ"
                     /locus_tag="ACIE68_002380"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFX5908687.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(20964..23303)
                     /gene="bcsB"
                     /locus_tag="ACIE68_002381"
     CDS             complement(20964..23303)
                     /gene="bcsB"
                     /locus_tag="ACIE68_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFX5908688.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(23314..25932)
                     /gene="bcsA"
                     /locus_tag="ACIE68_002382"
     CDS             complement(23314..25932)
                     /gene="bcsA"
                     /locus_tag="ACIE68_002382"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFX5908689.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(25929..>26637)
                     /gene="bcsQ"
                     /locus_tag="ACIE68_002383"
     CDS             complement(25929..>26637)
                     /gene="bcsQ"
                     /locus_tag="ACIE68_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFX5908690.1"
                     /translation="TTITAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWA
                     RAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICSGLQQLKASGR
                     YQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINDFRI
                     GSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLAN
                     WCLLNYSGLKTPVGSAS"
BASE COUNT         6204 a         7406 c         6823 g         6204 t
ORIGIN      
        1 ccagatcagg aatggcatat catgattgcg gcgagagcag gattgaatgt tctgaaaatg
       61 aaaaacaaca cgaaatccgt atttttataa attccatgga tattagtgcg ttatattttt
      121 tgacaggtta ataaacgtga tccaccgcac gctttgtcgc ccaccaggcg gagcgaatga
      181 ctacccttaa agaaaagccc gataattagc gacgaatttc ggaggttgga tccttatgct
      241 caatcagaaa attcaaaacc ctaatccaga cgaactgatg atcgaagtcg atctctgcta
      301 tgagctggac ccgtatgaat taaaactgga tgagatgatc gaggcagaac cggaacccga
      361 gatgattgaa gggctgcccg cctctgatgc gctgacgcct gccgatcgct atctcgaact
      421 gttcgagcat gttcagtcgg cgaaaatttt ccccgacagt aaaacctttc ccgactgcgc
      481 acccaaaatg gacccgctgg atattttaat ccgctaccgt aaagtgcgcc gtcatcgtga
      541 ttttgacttg cgcaagtttg ttgaaaatca cttctggctg ccggaggtct actccagcga
      601 gtatgtatcg gacccgcaaa attccctgaa agagcatatc gaccagctgt ggccggtgct
      661 aacccgcgaa ccacaggatc acattccgtg gtcttctctg ctggcgctac cgcagtcata
      721 tattgtcccg ggcggccgtt ttagcgaaac ctactattgg gattcctatt tcaccatgct
      781 ggggctggcg gaaagtggtc gggaagattt gctgaaatgc atggccgata acttcgcctg
      841 gatgatcgaa aactatggtc acatccccaa cggcaaccgc acctattatt tgagccgatc
      901 gcaaccaccg gtttttgcgc tgatggtgga gttgtttgaa gaagatggtg tacgcggtgc
      961 gcgccgctat ctcgaccacc ttaaaatgga atatgccttc tggatggacg gtgcagaatc
     1021 gttgatccct aatcaggcct atcgccatgt tgtgcggatg ccggacggat cgctgctcaa
     1081 ccgttattgg gacgatcgcg acacgccgcg tgacgaatcc tggcttgagg acgttgaaac
     1141 cgcgaaacat tctggtcgcc cgcccaacga ggtgtaccgc gatttacgcg cgggagcggc
     1201 ctcaggttgg gattactctt cccgttggct gcgtgatact ggtcgtctgg cgagcattcg
     1261 taccacccag ttcatcccca tcgatctgaa tgccttcctg tttaaactgg agagcgccat
     1321 cgccaacatc tcggcgctga aaggcgagaa agagacagaa gcgctgttcc gccagaaggc
     1381 cagtgcccgt cgcgatgcgg taaaccgtta cctctgggat gatgaaaacg gcatctaccg
     1441 cgattacgac tggcgacgcg aacaactggc gctgttttcc gctgccgcca ttgtgccgct
     1501 ctatgtcggc atggcgaacc atgaacaggc cgatcgtctg gcaaacgccg tacgcagccg
     1561 gttactgaca cctggcggga ttctggcaag cgagtacgaa accggtgaac agtgggataa
     1621 acccaatggc tgggcaccgt tacaatggat ggcaattcag ggatttaaaa tgtatggcga
     1681 tgaccttctg ggtgatgaaa tcgcgcgcag ctggctgaaa acggtgaatc agttctatct
     1741 ggaacagcac aaaatgatcg agaaatacca tattgccgat ggtgttcccc gcgaaggcgg
     1801 cggtggcgag tatccgttgc aggatgggtt tggctggact aacggtgtgg tacgccgttt
     1861 aattggtttg tacggcgaac cataatattt ttacagccag ccgctaactt cctgctggct
     1921 gtaaaattat cctcttcagg aggagatatt taacatcatt gccgcctggg tgcgattttt
     1981 cacttccaga cggcgataca gggattccag atgcgctttt accgttccgg tactgatatt
     2041 cagcgctctg ccgatctcct tatttgattc gcccgccgct aacatggtta aaatctcccg
     2101 ctggcgggcg cttaacgatt tgagatcttt aatgtccttt tccggcgtcg tccgccagtc
     2161 tccaggcaga aacatcatcc ccatcgccgc actatttacc gccaacgcaa atgtctcgac
     2221 ggttgaatca cgaggcacaa tggccagcac attaaaatga ataacttcct gtaaccaccg
     2281 tttattgcaa tccgtcgccg tgattaacac cttaacctca ggaaactgca ccacggtttt
     2341 ttgcagcaac cagtagcaaa actcaccatc ctgatcgcca tcgagcataa ctaaggcttc
     2401 agggtaactt tccagctttt gccataactc gtctgcctga ctggccccct gaatactcac
     2461 tcctggaata cgctgctgta aactgatttt cattccatga ataaatattg actgcctgtc
     2521 aaacatgact atttgcataa ctgaatctcc acctgaatac gttaaaaaga cttaagtagt
     2581 ggaagggtat tacccgcgag aaaaaataag aattcgccat ttggcggtgg ccattctaca
     2641 gagatgacgc gtagaaaata gttaccgata taaatagtta cagctaaacg cctgaaatta
     2701 catgtcgagg gcactattta aaacaatttt gaggatttcc ttatattggt ggttagtacg
     2761 catgcaatta aaaatgaaac tccgcgacca caagcaaaaa taacaaacgg caatgagaca
     2821 aaaataagca caaatagcca acacgtcctc tattcacttt aaagggaatc gctgaaaaat
     2881 acgctctgtt tagggggatt cacctttctc agaaagctat tccgcccttt tcctgctgag
     2941 aaatcgccac attcgtcatg acaacattgt gaaacccggc attagatgtt agaaaaaaca
     3001 ataaacaatg cgatatgcgc gttactgttt cgctgtgaac aacaatcggt caaagaaatg
     3061 gataaaattc acgcaatgca gttgttcatc aaagtcgcgg agctggaaag tttttcccgc
     3121 gcggcggatt tctttgcttt gccaaaagga agtgtttcgc gccagataca ggcactggaa
     3181 catcaacttg gcacccagct tctccagcgc accacgcgac gggtcaaact cacgccagaa
     3241 ggcatgacct attatcaacg agcaaaagat gtgttgagta atctcagcga actggacggt
     3301 ctgtttcaac aggatgccac cagtatcagc ggtaaattac gcatcgacat cccgccagga
     3361 atcgcgaaaa gcctgttact gccgcgcctg tcggaatttc tctatctgca tccgggaatt
     3421 gagctggaac tgagtagcca tgaccgtccg gtagatattc ttcatgatgg ttttgattgc
     3481 gtgatacgca ctggcgcgtt accggaagat ggcgttatcg cccgtcccct cggcaaactg
     3541 accatggtca actgtgccag tccgcactat ctgacgcgct ttggttatcc gcaaagcccc
     3601 gatgatctga cttcacacgc aatagtgcgt tatacaccgc acctgggtgt acatccgtta
     3661 ggttttgagg ttgccagcgt taatggcgtc cagtggttta agtctggcgg catgttgacg
     3721 gtaaacagta gcgaaaacta tctcgccgcc ggtcttgccg gtctggggat tattcagatc
     3781 ccgcgcattg ccgtgcgcga agccctgcgt gccgggcggc ttattgaagt gttgccaggc
     3841 taccgtgccg agccgctctc cctttcgctg gtttatccgc agcgtcggga gctttcccgg
     3901 cgtgtaaacc tgtttatgca gtggctggct ggcgtaatga aagagcacct ggactgaccg
     3961 actatacttt ttaagaatga ccacgacaga aggacaaaag agcggatgac gcaggaaaac
     4021 gagatcaaac gtcccaccca ggatctggag cacgagccga ttaagcagtt ggataatagc
     4081 gagaaaggcg gcaaagttag ccaggcgctg gaaaccgtca ccaccactgc cgaaaaagtc
     4141 caacgccaac cggttattgc gcacctgatt cgcgcgacag aacgctttaa cgatcggctg
     4201 ggtaaccagt ttggtgcggc tatcacctat ttctcatttt tatcgatgat cccgattttg
     4261 atggtgtcgt ttgccgcagg gggctttgtg ctggcctccc atccgatgtt gctacaggat
     4321 atcttcgaca aaattctgca aaacatcagc gatccgacgc tagccgccac gttgaaaaac
     4381 accatcaaca ccgccgttca gcagcgtacg actgtagggc ttgtcggcct ggcggtggcg
     4441 ctttattctg gcatcaactg gatgggtaac ctgcgtgaag cgattcgtgc ccagtcgcgc
     4501 gatgtctggg aacgctcgcc gcaagatcag gagaagttct gggtaaaata tctgcgtgat
     4561 tttatttcac tgattggttt gttaattgcg ctgattgtga cgctgtcgat cacctcggtt
     4621 gccggttcgg cgcagcaaat gattattagc gccctgcacc tgaacagcat tgagtggctg
     4681 aaaccgacgt ggcggttgat tggcctggcg atttccatct tcgccaacta tctgcttttc
     4741 ttctggatct tctggcggtt gccgcgccac cgtccgcgca aaaaagccct gattcgcgga
     4801 acatttctgg ctgctattgg ttttgaagtg attaaaatcg tgatgaccta caccttgcca
     4861 tcgctgatga aatccccctc tggcgcagca tttggttccg tgctggggct gatggcattt
     4921 ttctacttct tcgcccgttt gacgctgttt tgcgcggcgt ggattgccac cgccgaatat
     4981 aaagacgacc cgcgaatgcc agggaaaacg cagccttaaa ataggccgga tgcggcgtaa
     5041 acgccttatc cggcatacga ttttattcgg cctggctccc cgtaggccgg ataagatgcg
     5101 ccagcatcgc atccggctat aatgcgcaca taacctcttg aaactcttcc ccagagcctc
     5161 ttcagccatc tatttgggag caaacaattt cattccaact cataacccca gcatataaat
     5221 ccagttggta acttttattt aacctgaaac cagttttatc cactatttat aaaattatgt
     5281 gaagcatttc atagaagaaa aatcactggc ctaaacatta tccccttttt gcctggtttt
     5341 tgaccatttc cgcgatttgt tacacattga aatatcactt ttgctgtgcg taatatggct
     5401 attcgttagc caaaaaataa gaaaagatta tgcaagcaac agccacaaca ctcgaccacg
     5461 agcaagaata cacgccaatc aactcgcgta ataaagtcct tgtcgcctct ctcattggca
     5521 cggccattga gttcttcgac ttttacattt acgccactgc ggccgttatt gtgtttccgc
     5581 atatcttctt cccgcagggc gatccaacgg cagcaacgct acagtcgctc gccaccttcg
     5641 ccatcgcctt cgtcgcgcgc cccattggct ctgccgtttt tggtcatttt ggcgatcgcg
     5701 ttgggcgtaa agcgacgctg gtcgcctcgt tgctaacgat ggggatttcg accgtggtga
     5761 ttggtctgct gccgggctat gccacgattg gtattttcgc cccgctgctg ctggcgctgg
     5821 ctcgatttgg tcagggtctg ggcttaggcg gtgaatgggg cggcgcggcg ctgctggcga
     5881 ctgaaaacgc cccaccgcgc aaacgtgcac tgtatggctc cttcccacag ctgggcgcac
     5941 cgattggctt cttctttgcc aacggcactt tcttgctgct ttcctggcta ctgaccgacg
     6001 agcagtttat gagctggggc tggcgtgtgc catttatctt ctcggcggtg ctggtcatta
     6061 tcggcctgta tgttcgcgtg tcgctgcatg agtcgccggt gtttgagaaa gtcgctaaag
     6121 cgaaaaaaca ggtgaagatc ccgctgggta cgctgctgac caaacatgtt cgcgtaaccg
     6181 tactgggtac gttcattatg ctggcaacct atacgctgtt ttacatcatg acggtctact
     6241 ctatgacctt cagtaccgcc gccgcgccag ttgggcttgg cctgccgcgt aacgaagtgt
     6301 tgtggatgct gatgatggca gttattggtt ttggcgtgat ggtgccagtc gccggattac
     6361 tggctgatgc ctttggtcgc cgtaaaagca tggtaatcat caccacgctg atcatcctgt
     6421 tcgcgctgtt cgcctttaac ccgctgctcg gttctggcaa cccgattctg gtttttgcct
     6481 tcctgctgct ggggttaagt ctgatgggac tgaccttcgg gccgatgggc gcgctgttac
     6541 cagagctgtt cccgacagaa gtgcgttaca ccggagcatc gttctcttac aacgtagcgt
     6601 cgattctcgg ggcttccgtt gcaccatata tcgcagcctg gttgcaggct aactacgggt
     6661 taggtgcggt ggggttatat ctggcggcga tggctggctt gacgttaatc gccctgctgc
     6721 tgacccatga gacgcgacat cagtcgttgt aagtatcgtg tgggcagcca ccctcacccc
     6781 agccctctcc ctgaaaggga gagggagaaa agcggagcga gatgttatat cgcgaacata
     6841 acagccctcc cccagaaagt gaacggaaaa gcggtactcg ttgtttcaac acgcacaaaa
     6901 cagtcccctc gcccctttgg ggagagggtt agggtgaggg gaacacgctg ctcccgaact
     6961 caccagcaaa cacaatccaa cctcatcact tcttcatctg cgacaatatc gtccgacact
     7021 gattagcctc cccttcggaa ggtgagatca gtgccagtaa cgccgccgcc ggtgttacca
     7081 gcgttgccag tgccgcagca acagcaccac gggcaatcaa cggcccggct ttcaccccag
     7141 cctgcggatt tttaaacgtc ccacgcacat acagcggcga acgcagtgtg ataatccgaa
     7201 tccctttact ctccggatca atagtcaaat ccagctgttc cgaagcaaaa cttgccgtgc
     7261 cggtaacatt aatcaacgcg ttctcagtat cgaaagcaaa aatctgcggg cgcgccacgc
     7321 cgttggcaat attcagattc gccgccgcgc agttcacccg cacctcatcg tcaccaaata
     7381 tcgcaccgac aatgtagttg ccgacattca gcccaacaat ctccatcagg ttgcggctca
     7441 ccagcccgtc attcatcaac agtttcaggt tgccgttact gttgcctaaa agtgctgcca
     7501 ccgagttacc gctaccgcgt agttccgcgt caccgttcat ttcccccagc gtcttctgca
     7561 tcagttccac atcgggcatc agttctttca gtttcagtcg acgagcctga atatctgccc
     7621 gcccctgcat cggcttttta tcgccttcca gatgaatatt cgccgcaatg ctgccgcccg
     7681 ccatgccaaa tttcagcggt tgcaggcgca ggtcagcatt tttgaggatg atatgagtag
     7741 aaagatcgct aatcggcagg ctactgccat gctcaatgcg ccgccctttg aagcgaacat
     7801 cggcatccat aacgtcccat ttatcggttt cgaagcggtc ataaggcagc actttgcccg
     7861 caggctgaac gcttttttcg cccttcttct gttcagaccg tttcgacttt tctgcccctt
     7921 tcccggagtc aacgccaatt aacggtccca ggtccgccag ccgcaattgc cgcgactcga
     7981 catcaccttc cagttttggt cgtggctttc cggtggtgta gaccagagaa ccgtggatat
     8041 cgctatcgcc aattcgccca ttaaaaccgc gataatcaaa gaccgacgat ttttcagtgt
     8101 cgattttcgc taccagccga ccatccgttt caaacggcgg ggtatcgggc agcagaacgc
     8161 ccgtcagttc atagagatca cccagtgaat cgccagaaaa tttaagccgt aaatcgacac
     8221 cgcccatctt cattgggtca ttcacgacgc catcaaaagc aacacgggtg ttaccagagc
     8281 ggaaatcagc ctgtaccgga aacggcgtcc cttcgccacg cagcgccagc ataccgccta
     8341 tttttcccgt acccgtgagc ggttcaccgt tataacgtcc ctgcgccttc aggccaaaaa
     8401 cgtaatcgcc caccttttct ttatccgctt tacctttcga tccagtaact tcgctgaacg
     8461 gcagcggctt gcctaacgga tcaacaaaaa tctccagatc cgctttgctt actttgtcat
     8521 caatggcgat ccgcccttga tcgaaaagaa tattatccag ccgaaacgac catgccgacg
     8581 gctttgcatt cgcgtctttg ttatcatcgt tggcgagatt aaacgtccag ttattgttct
     8641 tttcggagag gcgaatcagg cgcgcgtcgg gcttttcgag cttgatccac ggcagccaga
     8701 ccgttttggt cagcagcgcc agcggggcca gcgttgcctc tacgcgtggc aaatgcacca
     8761 tcgtgacttc gggaatatcc ggtggattgc caagaatgat gtcttccgcg tgtacatggg
     8821 gccacggcac ccagctgcgc cagcctgttt cttgcttttg ccgctcccac accacgccca
     8881 gatcgccacg gatagcgaac ggacgattca actccgcaga gactttctgg ttgatggtcg
     8941 gtttgagtcg attccagtca aaagttgcaa tcaaaatgat cgccacgaca atcaacaaca
     9001 agaaagcccc tgaaatcgca gcggttattt tgcctgcctt gctcatcgtt cgcttcctca
     9061 tattcttcct gtgccagtcc taaagatagt ccagccaggc ggaaaatgag gcagcttata
     9121 gcgccagaac cgccgtattc agcgtttcta tcggtgccgg gcgtgaaaga aaccagcctt
     9181 gtgcggcgaa tgcgggcgag ttctgaacat cacgccactc ttccggcgtt tctacgccct
     9241 caacaatcac cccgcgacaa tagcgattca ttagatgtaa aagctgagaa aagagtgtgc
     9301 gtccttccgg cgactgacgc agcatcacaa acagttctcg cgcgattttg atgtagtcat
     9361 aacgcacttc acttagtgca gagaaatttg ccatcccggt accaaaatca tccagccaca
     9421 gcgggccaaa ttcacacatc gaggcaaagg ttgaatcttt cggcagacgg atatgctcca
     9481 ccagttcgaa acgcagccag ggaagacgct caatctggcg caggattttt ggttgctgac
     9541 gcagggcgat gagcgtaggg ccatcaatat tgaccgatgc cagcaggccg tgctctataa
     9601 agaagtcggc tttttgcgcc agcaaatcaa tctgctcttt cacaacctcc atccgatggc
     9661 tgacggtgat ttcagtaaaa tagcgatccg gcggcaggcg ttgcgaaggg ttcaagggat
     9721 gcgcgaccac cgttaatagc tccacggcca ttaaccgccc gcatgtttga tagatcggct
     9781 gccaggtgta agcacgctca cactgcaacc aaaaacgccg ttcctgcaag ctctcgatgc
     9841 ttgcttcagg gttgcttatt cgctggataa cctgccttat catcaaagat gtcctgtgtt
     9901 taaaagtgat gctgcccgga ctcgacaaag attatcggcg cgtaagggca gaactttacc
     9961 gcctggccgg ggtaaatttc ctcactccac aacggaatct ttcaggaata cgtcctggct
    10021 caaaaaaata atggaacact gttttaatat ggttgaccag caaaccacca cagcgcaaac
    10081 taacgctaat tttttacaga tcaggttcac gactatgtcc aaaaagattg ccgtgattgg
    10141 cgaatgcatg attgagcttt ccgagaaagg cgcggacgtt aagcgcggtt tcggcggcga
    10201 taccctgaac acttccgtct atatcgcccg tcaggtcgat cctgcggcat taaccgtcca
    10261 ttacgtaacg gcgctgggaa cggacagttt tagccagcaa atgctggacg cctggcacgg
    10321 cgagaacgtt gatacttccc tgacccaacg gatggaaaac cgtctgccgg gcctttacta
    10381 cattgaaacc gacagcaccg gtgaacgtac gttctactac tggcgtaacg aagccgctgc
    10441 caaattctgg ctggagagcg agcagtctgc ggcgatttgc gaagagctgg cgaatttcga
    10501 ttatctctac ctgagcggga ttagcctggc gatcttaagc ccgaccagcc gcgaaaagct
    10561 gctttccctg ctgcgcgaat gccgcgccaa cggcggaaaa gtgattttcg acaataacta
    10621 tcgtccgcgc ctgtgggcca gcaaagaaga gacacagcag gtgtaccaac aaatgctgga
    10681 atgcacggat atcgccttcc tgacgctgga cgacgaagac gcgctgtggg gtcaacagcc
    10741 ggtggaagac gtcattgcgc gcacccataa cgcgggcgtg aaagaagtgg tggtgaaacg
    10801 cggggcggat tcttgccttg tgtccattgc tggcgaaggg ttagtggatg ttccggcggt
    10861 gaaactgccg aaagaaaaag tgatcgatac caccgcagct ggcgactctt tcagtgccgg
    10921 ttatctggcg gtacgtttaa cgggcggcag tgcggaaaac gcggcgaaac gcgggcacct
    10981 gaccgcaagt accgttattc agtatcgcgg cgcgattatc ccgcgtgagg cgatgccagc
    11041 gtaaaagcag catatgcaaa aggctggata aagtgttacc cctctatcca gctcatccat
    11101 aaatacctga tgattgtgct aactcatcac catcaggcat tactgtgcag gtggaatatc
    11161 cgtcacttca ggatgtacgt catccgtggc gacagaggtg gtcgcagcgg cggtagatgg
    11221 ggccatgatt tgatcccagg ccgcctgcaa cgctttcatg ttaaattccg gctcgccttt
    11281 cggctgcagc agtattaacg ccatatcatt cgacaactgc tgacgcagat cctgatttaa
    11341 catctccacc gtcaggctgt taaggaaatc ctgccgcagt ttctgatact gctccggcgc
    11401 gatatcgacg acctgatttt gcaacgaacg catccgctga cccatcagaa tatcggtatc
    11461 agcccgcgca taggcggcaa acagtttctg cagctccagt ttcttttgcg ccactaacgc
    11521 attgaactct tcttccggca gacctttatc gcgaaccttc gccagttcac gcgccaccag
    11581 attaaggttg ctgttcagct tgtcgtttgg tgattcgatg ttgatggcac actgcgcacg
    11641 cagatacagc acacggcagt caaatccaag accgatgtct ttgctgttac tggcgcttaa
    11701 cgcttgctga acatgccaga acagcgcctc gcgggccagg tccgcacgcc agtagcgcag
    11761 cagtgcggct gattcacgaa tcggctgcca cggcgtatcc cacatgatag ataaccggtc
    11821 ctgacgcacc gcgtcagtca taatactcac cgcttccgca cgcagcggag aaagcgtcgg
    11881 caccggagct ggcgtttcac gtttgccttt cagttcgcca aacgttttgt tgatttggtc
    11941 gacaaccgag cgcgcatcca cgtttcccac caccagtagc gtcattgcat ccggggtgta
    12001 ccatttctga tagaaatctt taattttttc cgcttcaacg ggttgtttca gcggatcggc
    12061 aggatcgtga cctaacaagg ttgatccttt cagacgataa cgccaccagc cctctttagt
    12121 atcggcaggc caggttgcca ccatgtcctg actttgcagc gcgtggttga tggtttctgg
    12181 cgtgatagtc agtttgccag tggcatttgc caggtaagag agcgcctctt tcagcaaatc
    12241 gttacggtta ttgggcaaac tcagattaaa cagcgtagtg tcatacgaga caattaccgg
    12301 cggcatcggg cgtttagggt cgatcccctg ctgccacaat gaacgcgcct gtgctgcgtc
    12361 aaggccaccg ctttgcgtta gcgcaatacg agggatggcg tgactgtaac cgctctgttg
    12421 tgtactttcg gcgagcgaac cggtattaac cagcaggcga atttcaacac gatcgctggg
    12481 acgctggggg gtggtcagca cttgccactg taaaccgttg gaaagcgtcc cctgttgcca
    12541 tgctggatca ggctggagcg catctgcctg cacatagcca gcagtggcca tcatcagcaa
    12601 accgcccgct aaaagtcgaa tttttgtgcc ctgcatgtga acccctgatc aactatcctg
    12661 gtaataaaaa aactgcccgt tgtcggtcag tctaaatgac gttaaaaaca cttcgtgtta
    12721 gaccgcaaga acatgaaaat gtcacggaag aagtgaaata aacctgaact cacccaggtg
    12781 gtaagttcag gcacaggggt caattatgcg caaacacccg cactcgggga agggagtgcg
    12841 ggcataagtg atgagattaa gaggataatt cgtgcgtttt gccatccggc gcacgattat
    12901 tcagcacatc gtccagtttt ttgtggtcca gttctttcac ccacttagca acgacaatgg
    12961 tcgctacgcc gttaccgacc aggttagtca gcgcacgagc ttctgacata aagcggtcga
    13021 taccgaggat cagcgccaga cccgctaccg gcaaatggcc caccgcagag agcgtcgccg
    13081 ccagcacgat aaagccacta cccgttaccc ctgccgcccc tttagaagaa agcagcaaca
    13141 cgattaacag cgtgatttgg tggacgatat ccatctgact gttagtggcc tgggcgataa
    13201 acaccgccgc cattgtcagg tatatcgatg tgccatcaag gttaaacgag tagcctgtcg
    13261 ggatgaccag ccccaccacc gatttgcggc agccgagttt ctccatcttg tcgagcatac
    13321 gcggcagcgc cgactcggaa gatgaagtcc ccagtacaat cagcagttct tcacggatgt
    13381 agcggataaa tttgaagata ctgaaaccag tcgctttagc gattgaaccc aataccagca
    13441 ccacaaacag gatacaggta atgtagaaac agataatcag ctgccccagt tgcaccagtg
    13501 tgccgacgcc gtatttaccg atggtaaacg ccattgcccc gaacgcacca ataggcgcca
    13561 gacgcatgat catattgatg atgccgaaga tgacctgcga gaaactttcg atgacgttaa
    13621 aaatcagttg gcctttgctg cccagacggt ggagcgcaaa accaaacagt acggcaaaca
    13681 gcagcacctg cagaatgtta ccgctggcaa atgcgccaat gacgctcgcc gggatgacat
    13741 ccataatgaa ggcgacaatg ccctggtctt tcgcctgatc ggcgtaaacc gctaccgctt
    13801 tcgcatcaag cgttgccgga tcgacgttca ttccggcacc aggctgcacg acgttaacga
    13861 tgataagacc aataatcagc gcgatggtac tgacaatttc aaagtaaagc agtgcgactg
    13921 cgccggtacg accgaccgcc ttcatgcttt ccatgcccgc aatgcccgtt acgacggtac
    13981 aaaagatgac aggagcgatg atcatcttaa tgagcttaac gaagccgtcg ccaagcggtt
    14041 tcatttgctc gcctatttca ggatagaaat ggccaaggag aataccaatg gctatcgctg
    14101 tcaggacctg aaagtaaagg cttttaaaca gagaggtttt catagggtgt cctttagtaa
    14161 aaccacaggt cttgtaaggt tatggggtac ctgcggcctt aaaataacac ccagacaaca
    14221 tcacagaaat gtacctggat cataattgaa acaaaaaggt taaaaagttt gagctggctc
    14281 gcacaaacca gcacttttta aagttttgta atcagtttgg ggtagctact tttcttccag
    14341 gtaactctct tcgaagattt caatagggag tgggcgagca aaaaggaagc cctgggcaat
    14401 accaacgccc gcttttgcca gccagtcgcg ttgtgcttca gtctccacgc cttcggcaat
    14461 catttgtaag ttcaggctct gcgccagcat gataattgca gcaatcatgc tgctatcttc
    14521 cggcaagcct tcaacaaaca ttttgtcgat ttttagtacg tcgattggca acgatttcat
    14581 atgctgcagc tgacgcagcc ctgcgtagcc catgccgaaa tcatccagcg ccacccgaac
    14641 tccggcattg cgcagcggac ggaggattgc caccgcagca tgagggtcgt caatacgtcg
    14701 gctttctgtc acttccagaa tcagtgttcc cggctgaatg cgatagcggg ttaacagttc
    14761 cagcatatcc gccaccatat tcgggtgcat cagttgcagc gcagagaggt ttaccgacaa
    14821 gggcagcata atgccgcgct cttgccaggc tgcaagcaat cgacaggact cttccagcac
    14881 ccagtgaccg acggtaacca tcagcccaca gcactcaatg cgatcgatta agccatccgg
    14941 caggtcccaa ctgccatccg gttgctggat acgcagtaac acttccgcac tgaccagttt
    15001 tccgctggtc atctcgacct gtggctgtaa ccaaatagca aactgatgat tttccagtgc
    15061 attaaggata tcgctctctt ccgtcaaccg cttctgggcg gcttccatct gctgcggatc
    15121 aaagaactga atctgattct tgcctttatg gcgagcggta aatgccgcag aaatagcgcg
    15181 actgtaaagc tgttcggcgg tgagatcgcc gtagaacatc gccacgccaa tgctacagtg
    15241 cggacggagt tgaatacgtt caatcggcag gcgctcgctc atgatagtga gcacttgctg
    15301 acctaaggtg attgcgtgcc acggttcctg tacaccgttg gcaatgacag caaagtcata
    15361 accgctaatc tgcgcgagga tcatacgtgg cgacagtacc gatttgagtt tttccaccag
    15421 cgtcagcagc agaatttctc gttgcgcctc tttcagcacg cccgcagtat cacgcagggt
    15481 ttcacaggtg atgatcatca gcgcggtggt ttgtttacgc gcgacaactt gctccagcat
    15541 ctccatcagc aaggctttgt tcggcaaatc cgacaccggg aagcgcatcg cattttcgtt
    15601 ctgttcttca taatggcgct gcagcaattg ctggttgagg ttgtaactgc gcaccaacat
    15661 accgatttca tcgtcctgat gcagacgcgg taatgccagt tggtgaccaa caagctcctg
    15721 ggctgggatg gcgttaagtt cgcgagcaat attgcgtaac ggatgcaaaa tcaggcggtt
    15781 aatgcaccag ctgatggcga cggtcaggat aagcgacaaa agtaagtaaa tggtcactaa
    15841 cgttgagagg gtgctcatca cgaacttata catacggaag gaatccgcct gtagtaccag
    15901 ataggcaatt ggctgcgggt ttgccggacg ttcgagcgag taaacgccca gcgagatttg
    15961 aaccggtagc tcaaacaggc gagtaaccat taccggcacc gggcgctctg gaataaaact
    16021 tttacgcagc gcctggaact ggttaggcag cactacatcg gcacggctga ccacgcctgc
    16081 cggtttaatg ctggcaagaa tagcttccgc ttcgggaata tcgcctttca aaatagctga
    16141 agataagggt tcgcggacag agcgagcaat gctttccagt tgcgtagccg tgttatagcg
    16201 attctgctgg accagatgga acagcaaaac ggtgcaaaaa ataaaaacga acaccaggac
    16261 aacggctgcc accattgcca tctgcttgat tgttaacgag cgacttacgc gcaaattgtc
    16321 tccccccgga aatcccagat tgccgcccga gtatacccga tcgcagcggc attaagagag
    16381 gcgctatctg aaaacttacc agtcggcgta aggtatcagc ggctgcggcg gtaaatccat
    16441 gtcaccctgc catccggcgg cggaataacg tacgtagagc aggaaatggc tgggtgcgta
    16501 atctttcgcc tgctggatat caattgccgt gccaacaaac cagttggaag taacacgtcg
    16561 ttcaagtaat gcccgcgccg tgtagccgaa gccctgactg ctgccgccat cgttggattg
    16621 ccgcgcagct tcttcctgcc agtcggtcgg gatcagattc atcagcggat aacgcggcat
    16681 ggttttggtg cgtgaatgcg accacgagcc agacgccccc agctcccacg accagttttc
    16741 cgtgcgctcc cgccacatca ccggtatggc aaacgacagg tattcctgcg gactgtagta
    16801 accgccctga ccgagtgagt agccactcag atctttgtcg taatgccaga tcatgttgtt
    16861 caggccgatt gtgacgcggc gattgttctg gttaatgacc ttatagtaat agcccgtcat
    16921 ccagcgcacg cgccagttat cttcgacatt tttaccggtt aactggtcgc cactaagcga
    16981 tgcccagacg ccgtttgctt cacctttatc gtagctcaga cttagcccca caccgtcggc
    17041 acgtacgcca ccccattttt tcccggtatt gctcggggag tctttttgcc caccaaaggc
    17101 cagcaaagaa ctggagatgg gccgacggtg ggcgttaacg gtgtaaccca gcggcccgat
    17161 atcatcgctg taactgatgc cgccgaccac atccaccacg ttgaagccca tcggcgtggt
    17221 accgatatcc cagctccaga cgtcatttcg ccagccgacc gccacgctgg caccggaatc
    17281 cgactggctg cggttgccgc tacagtcctg taatgtacag gtgccccagt tatcatccca
    17341 tttaccatcg gcattagtgg agaaactgcc gacgttcata ttgacgaaat cactgcgaaa
    17401 gaacatccgc ccgtcagaat acggcgcatc cacctgcaac atggtagtgt gcgctttcag
    17461 atcggagtaa ccaccggtgc cgctcgaacc ccagtaatca tgctcgaggg tgacgttaag
    17521 atcctgctgg cgatagaggt ccgccgcatc gctgcgcacg ccgcgtttca gccagtcatc
    17581 tttctcgtcg ttacgggtca gtcgggtaaa ggtgtcgtta tcctgcggac gcgtcgtagt
    17641 cacaccggat gcgaccatgg cgtctttgta ggtttccagc gcctgctttg gatcgcccgc
    17701 ctgcgcttca aacttcgcac cgtcgcgcag caccatcgcg ctttccatcg acggtggctg
    17761 agattttgcc tgcgggatca acttattaaa cgtctgctgc gccgctgcgg tatctccaag
    17821 ctgcgcctgc gccagcgcca cgcgccgctg tgtgttcagc gaggcgttat cggtagcggg
    17881 cagtttcgcc agctggctac gtgccgccgc tgtgtcaccg gcagcaatat ccacttccgt
    17941 cagccccaga atggcatcgg cgttagttgg ctcccgcgtc aggacattct gatatgcagc
    18001 gcgggcggcg gtgtaatcac gtcgctgctg cgcccagtca gccagcgtga ggtcaatacg
    18061 cgtggaaggt ggttgctgac gcagcatcgc ttccgcttca gcttctttgc cgctttctcg
    18121 caggcgatta gcggtttcca gcacctgatc gctttgcagt cgattaacca gctcctgaat
    18181 attgctgttc cactgcgcgc gcggcaggct gttgatatgc gccagcgccg ctctgtcctg
    18241 gtcatgacca gagaggtaca gcccgtaagc gtaaacctgc tccgggtcgt tcggcttctg
    18301 ctgcgccaga ttgcgcatta acgtatcggc ctggctgcgt tgtccggcct gccagagatc
    18361 ctgcgaaagt cggtaagtaa tccatacgct gccggggtcc agcgccagtc gttgccgctg
    18421 aagtgctgcc gcctgcgccc atttgccctg gttttccagt gcctctgcct gctgtgccag
    18481 acggtcgttt tgcaggctgc gttcgatatc atcaatgcta cgccgctgac tggcagagag
    18541 cgaggcgata aacgcttcag ctttttctgg cgattgctgg cggtaaatat ttgccagccc
    18601 gcgcacggcg ttagtgttgc cgctgtccat acgcaaggtc tgctgataat aacgttcggc
    18661 ggcgggataa tcttttcgcg ccatcgccac atcgcccagc cccagcactg cataactgtc
    18721 ggtgttatcg acattacgcg cctgctggaa caggcgttct gcccggtcag gattattggc
    18781 tttcagcgca gcatcgccct gctggatcgc cagccagtag cggtttactt tcagcagact
    18841 gttccatttg tcgttgttgc tgctgtgcgg gtccagtgcg agggcttttt ccagattcgc
    18901 cactgcattg gcgcgatcgc ctttctgaga atacgcctgg cccagcgccc ccagagcttc
    18961 actgtctttc gggttcgccc gcaccgcctg ttgtagttcg ggaatggctt tacccgccat
    19021 accagagtcc accgccgcta aaccttgcgc acgagcgcgg aaagcaggat cggccagctg
    19081 tttttgctgt tctgccagtt gcgattgcgc agccgccacg ctatcgccat cactaaagat
    19141 cgagagatat tttttcagcg ccgacacact ggcatcactg acgggcatgt ctttaatctg
    19201 cccgtaccag attttagagg ccccttcgcg cccggcgttc gattttgcca tctgttccag
    19261 gacggcaaaa ccttcgtcac ggcgatcgct actaaacagc aatagcgcca gattgttttg
    19321 caggcccgta ttgcccggtg catccgcatt gatgcgtttt aactgattaa tcgcttcgcc
    19381 acggcgagcc ggaattttcg ccaccgtact ccagtactcg acagcaatgt caccttccgg
    19441 cggcgcaccg ttgaacagtt tgttgtaact cgccacagct tcttctgcat gaccggtcgt
    19501 cgcctgcaat cgtgcctgtt gcagtgcctg acgaccatcc ggcgtggaaa gtagcatcgt
    19561 agtccgcgac gatttatacg catttgaact cggcgctaac tgcgacagcc gatcgagctg
    19621 tttttgcgcg ccatcaatat cgccctgacg taacaaagaa cggaaacggg cggcaacgac
    19681 gtccgggtta ttcggatcaa taagttccag ccgatataac gactgttgca ccagatcttc
    19741 acgatgggtc gcttcgccta accgaacttg ctccagcaac tgttgctgag cggttggtgc
    19801 tgcctcgacc atcggcatga cggccagacc gagggaaagc gtgaatatgt ttagtgtgaa
    19861 tttgcgcatt cctggcccca gtcaggtaat aactcacctt ttgtcgagaa gcggaaacgg
    19921 tgttgatccc agccttgtcc aaacagggtc agcacatagt tgtaataggc atcgctgccg
    19981 ggaaagttat cggccacgcg ctggcgctga acggcctgcg catcgcggtt ttgtaaaaag
    20041 ggcagcatgg cggcagaaaa accgaccggt cctttaccct gcgctttccc cgtagccaca
    20101 tccacttttt ccggcggata accgtttttc tcagtgaatg tcgccatcgg tttaaaccgg
    20161 ttgagcatcc gcgctttttg cggatcgctg tcaggcatca tgcctaccca catgtaaacg
    20221 cggatagcgt cgtagctgct gatcaatgtt ttttcggctt ttagctgcca gcctttgtct
    20281 ttctcatagc gcacccagtc tggcgaaaaa cctttcgggg cggtttccag caataaacgt
    20341 tgattggttt cgcgcaacgt ggtccacggc gcgccaaagc gggtgaaata ctgcgccagc
    20401 gtcggcggca ggtagctggg gttaaaacgc cagctgttat cctcagcaaa acccactttg
    20461 cccggtaaca acatggaacc cagcccaggc accgtcacca cttcctcccg cgcgatacgt
    20521 tttagcaacg cgctgccgat gtcggtataa cgctgctctt tccacaaacg ccccgcctcc
    20581 agcaacgacc aggccatcca gacatcacca tcggaggccg aattgctgtc cagcacttcc
    20641 cacttactgt tctctttctt gccccacagc caggcgggca aacgttcttt taaagagccc
    20701 tgagcgagat tgttctgcgt ccagtcgaga atattatcga aagctacacg gtcgttagcc
    20761 gccagggcaa agaacatacc gtaactttgc ccttcggagg tggtgatttt gcgcgcgtcg
    20821 ctggggtcga tgacgcgccc ttcctgactg atgtaatcct ttttaaactg ctcccaggca
    20881 ggccaggtac aggctgcctg aacactaaag gcagccaaca gcagcatcgt cacgattcca
    20941 ctacgcaaca cattcatctt caattactcg ttatccgggt taagacgacg acgactaata
    21001 attcgcagca gacgccacag tacccatgcc agcaatatca cactgatagc cgccagcacc
    21061 gccagcagaa tcggatggtt tgccagcgca taccacaagc gctcgaacca cggcagatga
    21121 cctacgtaat aaacgtcgcc aacacgtagg ctgttgatac cggactcgcg gatcaccgcg
    21181 accgaaccga acatggtggc gcgtttgccg ctatcgttca ccgcatcgtt aagcatttca
    21241 taaccgcgtg ggctatctgc caacagcgca atcacgctgc gctggtcgtt atacggcgac
    21301 tggaagccaa tcaccgccgc catcgcaccg gaagaggtca gcgttgaccg ggtttctgcc
    21361 gcgcgatcgc tctcgtccgg cacaatgccg gggaacgggg tctggcgcat cggtgttttc
    21421 acccagcttt cggtcgcctg caccaatagg tcgatctgct tatcgtcttt cagtttgtcc
    21481 gggataccac cgatgatcat gatgtcggca tctttgccct gaatggtgct gccatcatcg
    21541 gtcaccgtca gattaatcgc cgggaagccc gtctgtgcgc cgataaaacc aacagtattc
    21601 agcaacgttt ccatctgtgc ttcgttaggc gctttcggca tcacggtgat ggtttgcgac
    21661 agatccgcca tccggctgaa tgggaagccc gcgttagcaa aggcgcgtag atccggcatc
    21721 gggatgaagt ggtaatactt cgagaagtcg atggtggaat cgtcaccaat caccacatga
    21781 ttctgcaccg gctggaaggt aatacagtta tctaccgaac cgcctggcat cgggttcata
    21841 tactcaaagt cgaagcgcag ttggttggtc gcgcccagtt tcagcgccgg aatagagaca
    21901 tctgttttgc catccagcaa accttgtaat accggaatcc gcagcagcag gcggttcgcc
    21961 tcctgtttgc tgctcaggtt gaaggattgc aggaactggt tattcaggct gatatccatc
    22021 cgcgaactgt ctttcaccgg cggcatggtg tagcggtaat taatatccat atcaatgccg
    22081 gtactgcgca tcaggtagag atccggcggc aggtttagcg aaacgttaat cgctgctggc
    22141 tcaagaccgc tggattgtaa ctgttcttca taggttttca gttcgccaaa tgtgaccgga
    22201 cgatcggtac gtacccagtt cggcgcatcg tacggcttac gcggtagcag cggtttcact
    22261 tcattcacta ccacgctttc accacggaac agaatgttac cctgagcgat acctttcgct
    22321 gcctgcaaca ggtctttgtc gtcacgacca aacaccacca gcagtttgac gtaaggattc
    22381 tgcggatggt taatcatctc aatcacaggg gcttttaccg ccggatgatc gcgcaggaag
    22441 tccggccgtt tgtcgttggt tgcaaagaca atggcattac gatccggcag ttggttatag
    22501 agcaccggga agttctgccc acgccagcca gaacgcgaac caaaccacga ggcgacaatg
    22561 gcagaggctt gttgcagccc aacatccggc gcacccgcaa agaccattgg caaggtgtta
    22621 gtacggttat cgcgcgggtc aaagaatggc accgggaagt gtgacaggtc attcttcaca
    22681 ttcagggtct gataggtcag atccagtcca ctgctccgcc caacatccag ccaaagcgtg
    22741 gtgctggccg ggttttcgca cacgtcctga taatggccga caaactccag ccgcacacgg
    22801 ttgaagtcgg taataaacag tgggttaatg ggcatttgcg ccagcgtttt tttacccaac
    22861 tgttctttgg tcactggcag cacgcccatc agctcatcat tgagataaac ctttaactgc
    22921 gactggacag gcagtaacga tggcgatggc gtgtattcga ggttgagcat cgccttcgtc
    22981 accacttcat cgctgcgcat accaaactca atgctgccgt tcgggttaat gccacgtagc
    23041 accatgctgc ccggcggcgg tgcaatttgc gcaaaggtca gcttcacatc acgcgaagga
    23101 ccgttctgcg ccacgactgg cgcatcagcg ccctgcacgc ccggcatcac ttgccccacc
    23161 tgcggatttt gttccgtctg ggcggctaca gctggctcag cattgatcag tggttgcgtt
    23221 gctggcgtcg cctgcgtcat gaaagagggg aacgcactca tccccatagc cactgcacaa
    23281 atccagaata gttttctttt catcgcgtta tcatcattgt tgagccaaag cctgatccga
    23341 tggttgtgcc gtttcgctcc gctccgggcg gcgcggaata aacgatacaa cccaggaaac
    23401 cagagaagtc agcacacgga atatgccctt caccgaagaa ggcgcaaact ccgccagatg
    23461 gcggtagcca cggaagccga gcttcagaat atccagcaga ctttccagcg gcttatcttc
    23521 cgggtagctg tcctgccaga gcgcccatgt atccgcacgg gcaaacgtac actgcacaaa
    23581 atcgatatgt tgctgggtgg tgagcggcat taatttcagc ccaacttcat tacccatcac
    23641 gcgcgccacc tgggtcggga agacgtattc ctgctgaccg cgtttaagca acagattcac
    23701 tttctgccct tccagaatct gcgcctgacc gttgatcttg atccccaaac caccgtcgga
    23761 gaaatcctga acggtacacg agaagaggtg accatcttcg cgggcaattg ccgcgggcat
    23821 cgtcatctcc acgcggtgcg atcggcgtac ctgtttgctt tctaccgata ctgcaactgc
    23881 gccgccaaga acaatcaggt tgtagaatac ccacaccata ctgacgacca cggtgagcat
    23941 ctcggttggc gggccataga agtagcgcca gatgcctacc gcaacgccca ccaggttgag
    24001 caggacaagg aagatgtagg gccgcgagat cacccagtcg acgtactctt cttccaccag
    24061 tccacctttg gcggtgacgt taaatttgcc tttgtgcggg ttaatcagcg ccaccagcgt
    24121 cggtggtgcg atataccacg ccagcaccgt ttcgtagatt tcactccaga aagagtggcg
    24181 atatttgccc tggatcttgg agttggtcag gctggcatgg atcatatgcg gcagcacgaa
    24241 cagggcgatc atcaacgctg gcgcatagat gatgtaggca tgaagcagca ggaacgccag
    24301 cggcgcagtc aggaagatca gtcgtggaat gcccgacaag aagtggaaca tggcgttgac
    24361 gtagcacagc cgctgggcga atttcagccc tttaccggta agcgggttat cgagacggaa
    24421 gatttgcacc atcccgcgtg cccagcgaat acgctgaccg atatgcgccg acagactttc
    24481 ggtcgccagc cccgccgcct gcggaatacg catatatgca gaggtgtagc cacgacggtg
    24541 caggcgcaga gacgtgtgcg catcttcggt taccgtttcg acagcaatac cgccaatttc
    24601 atccaatggt ttgcgacgaa ttactgcaca ggaaccgcag aagaaagtgg cgtcccacat
    24661 atcgttgcca tcctgcacca gaccatagaa cagcgtgcct tcgttcggcg ttttacgaaa
    24721 acgccccagg ttgcgttcaa acgggtccgg cgagaagaag tggtgcggcg tctgcatcat
    24781 cgccagctgt ttctctttca ggaaccagcc catggtcatt tgcaggaacg atcgcgttgg
    24841 tacgtggtcg cagtcgaaaa tcgacacgaa ctcgcctttg gcatatttca gcgcattgtt
    24901 gatgttgccc gctttcgcat gttcatgagt ggtacgggcg atatatttca cccccacgtt
    24961 ttgcgcaaac tggcgaaact cttccctacc gccgtcatcg aggatccaga tgttcagctt
    25021 gtctttcggc cagtcgatac ccagcgaggc gtaaatagta tttttcacca cgttgagatc
    25081 ttcgttgtaa gtcgggacaa agatatccac cgacggccac agcgacatat ctttcggcaa
    25141 tggcaccggc tgacgattca gcggccatac tacctggaag tagccgagca ccagcacaat
    25201 ccacgcgtac gtttcagcga agagcagaat aagcccgcac accaggctga ccggatcgtc
    25261 ccagttcagc gtagaggtgt aacgccacca gatataacgg caagaaacgg tcagcgacag
    25321 cacaatcaac attagcgccg agaagcgccc cggcatccgc cgtacgatca gcgctacacc
    25381 ccacagcagc atcaggaaga taaactgcgc cagcgggtta aacggttgag taacgcagat
    25441 taacgccaga atcagcgaga aggtgacgat gataccgagg atcaaccgcc gcgccccggc
    25501 actcaaatga ccgagctctt ttttctcatc aagatgctgt gttttatggc taacgcgctc
    25561 aggcagctcg ttcatccatt gatggtaacg tccacgaata ttctgcagac ctgaaaatgc
    25621 ccgcctgcgc ggtttcggcg tttctttgcg cgatgtaccg atcagtaacc agcatgtttg
    25681 aatgagataa cggaccgggt ccagcggacg cggacgcgag gcgttgatat gcggatacag
    25741 gtttttatgt tctgcgcgaa tacgctgcca gcgcgggtgc tccagcggaa taaaaatcca
    25801 ggccaggatc atccagaaac agccgagcgt cgcgctgaaa gccgacgcac cgtgacgacg
    25861 ataatcgcga taacgcccga taagccgcgc gttgaccggc gggataagca accaccgggt
    25921 caggatactc atgatgcact cccgactggc gttttcagcc cggagtagtt caacaggcac
    25981 cagttcgcca gcgtcagtat ctcttcagcc gccagcgcat cactgcgata ttcacctact
    26041 ggttgcttag ccgccaggca ttcagccatc gcttcatcac gatgaatgag catcggcagt
    26101 aatcggcgct ggctttgcaa ccaaagctgg taaatatcgt cctgaacctg actgccaata
    26161 cggaagtcat taatcaaaat atgtgcgcca tccggcagcg cttgctgatg caggcggata
    26221 tggcagttgg catccacatt gacgatagcc agcgagtgat cgcacaaact cagcagctgg
    26281 tgggttatct gcgaggcatc acgcggtaag tcgattaaaa tccactggta acgcccgctg
    26341 gcttttagtt gctgtaagcc ggagcaaata tcgctcagcc gggtttgcca gtgctgtgga
    26401 ttttcttgtt cttcaatgga taactgacca aaaggcagca aatcgagctg cgaggtgtag
    26461 cgcaacccag cgtcacgcca gtcctggcca tccagcatcg ctctggccca gccctgacgg
    26521 tgggtaaaat caacattaaa tgacaggcgc aacaagttgt ccgggcaggc atcgaccacc
    26581 agaacatttt ctcccaacat ttgtaatgac caggctaatg cggcggtgat ggttgtt
//
LOCUS       DBANUU010000058        26416 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000058 DBANUU010000000
VERSION     DBANUU010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.058"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            15..1484
                     /gene="gspA"
                     /locus_tag="ACIE68_002384"
     CDS             15..1484
                     /gene="gspA"
                     /locus_tag="ACIE68_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFX5908691.1"
                     /translation="MSTRREVILSWLCEKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSIGRLTEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIALTCGWLLLSSFTATLPVPAWLIPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTHKWFESVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1486..1905
                     /gene="gspB"
                     /locus_tag="ACIE68_002385"
     CDS             1486..1905
                     /gene="gspB"
                     /locus_tag="ACIE68_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HFX5908692.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWVLWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPGVA
                     VENAPSSSEDEENTVEESDEKAGLRERVKNALNELER"
     gene            2143..2454
                     /gene="rpsJ"
                     /locus_tag="ACIE68_002386"
     CDS             2143..2454
                     /gene="rpsJ"
                     /locus_tag="ACIE68_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFX5908693.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2487..3116
                     /gene="rplC"
                     /locus_tag="ACIE68_002387"
     CDS             2487..3116
                     /gene="rplC"
                     /locus_tag="ACIE68_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFX5908694.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3127..3732
                     /gene="rplD"
                     /locus_tag="ACIE68_002388"
     CDS             3127..3732
                     /gene="rplD"
                     /locus_tag="ACIE68_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFX5908695.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3729..4031
                     /gene="rplW"
                     /locus_tag="ACIE68_002389"
     CDS             3729..4031
                     /gene="rplW"
                     /locus_tag="ACIE68_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFX5908696.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4049..4870
                     /gene="rplB"
                     /locus_tag="ACIE68_002390"
     CDS             4049..4870
                     /gene="rplB"
                     /locus_tag="ACIE68_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFX5908697.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4887..5165
                     /gene="rpsS"
                     /locus_tag="ACIE68_002391"
     CDS             4887..5165
                     /gene="rpsS"
                     /locus_tag="ACIE68_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFX5908698.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5180..5512
                     /gene="rplV"
                     /locus_tag="ACIE68_002392"
     CDS             5180..5512
                     /gene="rplV"
                     /locus_tag="ACIE68_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFX5908699.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5530..6231
                     /gene="rpsC"
                     /locus_tag="ACIE68_002393"
     CDS             5530..6231
                     /gene="rpsC"
                     /locus_tag="ACIE68_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFX5908700.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6244..6654
                     /gene="rplP"
                     /locus_tag="ACIE68_002394"
     CDS             6244..6654
                     /gene="rplP"
                     /locus_tag="ACIE68_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFX5908701.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6654..6845
                     /gene="rpmC"
                     /locus_tag="ACIE68_002395"
     CDS             6654..6845
                     /gene="rpmC"
                     /locus_tag="ACIE68_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFX5908702.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6845..7099
                     /gene="rpsQ"
                     /locus_tag="ACIE68_002396"
     CDS             6845..7099
                     /gene="rpsQ"
                     /locus_tag="ACIE68_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFX5908703.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7264..7635
                     /gene="rplN"
                     /locus_tag="ACIE68_002397"
     CDS             7264..7635
                     /gene="rplN"
                     /locus_tag="ACIE68_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFX5908704.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7646..7960
                     /gene="rplX"
                     /locus_tag="ACIE68_002398"
     CDS             7646..7960
                     /gene="rplX"
                     /locus_tag="ACIE68_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFX5908705.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7975..8514
                     /gene="rplE"
                     /locus_tag="ACIE68_002399"
     CDS             7975..8514
                     /gene="rplE"
                     /locus_tag="ACIE68_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFX5908706.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8529..8834
                     /gene="rpsN"
                     /locus_tag="ACIE68_002400"
     CDS             8529..8834
                     /gene="rpsN"
                     /locus_tag="ACIE68_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFX5908707.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8868..9260
                     /gene="rpsH"
                     /locus_tag="ACIE68_002401"
     CDS             8868..9260
                     /gene="rpsH"
                     /locus_tag="ACIE68_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFX5908708.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9273..9806
                     /gene="rplF"
                     /locus_tag="ACIE68_002402"
     CDS             9273..9806
                     /gene="rplF"
                     /locus_tag="ACIE68_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFX5908709.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9816..10169
                     /gene="rplR"
                     /locus_tag="ACIE68_002403"
     CDS             9816..10169
                     /gene="rplR"
                     /locus_tag="ACIE68_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFX5908710.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10184..10687
                     /gene="rpsE"
                     /locus_tag="ACIE68_002404"
     CDS             10184..10687
                     /gene="rpsE"
                     /locus_tag="ACIE68_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFX5908711.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10691..10870
                     /gene="rpmD"
                     /locus_tag="ACIE68_002405"
     CDS             10691..10870
                     /gene="rpmD"
                     /locus_tag="ACIE68_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFX5908712.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10874..11308
                     /gene="rplO"
                     /locus_tag="ACIE68_002406"
     CDS             10874..11308
                     /gene="rplO"
                     /locus_tag="ACIE68_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFX5908713.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11316..12647
                     /gene="secY"
                     /locus_tag="ACIE68_002407"
     CDS             11316..12647
                     /gene="secY"
                     /locus_tag="ACIE68_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFX5908714.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12679..12795
                     /gene="rpmJ"
                     /locus_tag="ACIE68_002408"
     CDS             12679..12795
                     /gene="rpmJ"
                     /locus_tag="ACIE68_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFX5908715.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12942..13298
                     /gene="rpsM"
                     /locus_tag="ACIE68_002409"
     CDS             12942..13298
                     /gene="rpsM"
                     /locus_tag="ACIE68_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFX5908716.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13315..13704
                     /gene="rpsK"
                     /locus_tag="ACIE68_002410"
     CDS             13315..13704
                     /gene="rpsK"
                     /locus_tag="ACIE68_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFX5908717.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13738..14358
                     /gene="rpsD"
                     /locus_tag="ACIE68_002411"
     CDS             13738..14358
                     /gene="rpsD"
                     /locus_tag="ACIE68_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFX5908718.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14384..15373
                     /gene="rpoA"
                     /locus_tag="ACIE68_002412"
     CDS             14384..15373
                     /gene="rpoA"
                     /locus_tag="ACIE68_002412"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFX5908719.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15414..15797
                     /gene="rplQ"
                     /locus_tag="ACIE68_002413"
     CDS             15414..15797
                     /gene="rplQ"
                     /locus_tag="ACIE68_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFX5908720.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15904..16272
                     /gene="yhdN"
                     /locus_tag="ACIE68_002414"
     CDS             15904..16272
                     /gene="yhdN"
                     /locus_tag="ACIE68_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFX5908721.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            16283..16708
                     /gene="zntR"
                     /locus_tag="ACIE68_002415"
     CDS             16283..16708
                     /gene="zntR"
                     /locus_tag="ACIE68_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFX5908722.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16764..16982
                     /gene="arfA"
                     /locus_tag="ACIE68_002416"
     CDS             16764..16982
                     /gene="arfA"
                     /locus_tag="ACIE68_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFX5908723.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16979..17389)
                     /gene="mscL"
                     /locus_tag="ACIE68_002417"
     CDS             complement(16979..17389)
                     /gene="mscL"
                     /locus_tag="ACIE68_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFX5908724.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17519..18895)
                     /gene="trkA"
                     /locus_tag="ACIE68_002418"
     CDS             complement(17519..18895)
                     /gene="trkA"
                     /locus_tag="ACIE68_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFX5908725.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18917..20206)
                     /gene="rsmB"
                     /locus_tag="ACIE68_002419"
     CDS             complement(18917..20206)
                     /gene="rsmB"
                     /locus_tag="ACIE68_002419"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFX5908726.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGTEEGDGFFYAKLIKK"
     gene            complement(20252..21199)
                     /gene="fmt"
                     /locus_tag="ACIE68_002420"
     CDS             complement(20252..21199)
                     /gene="fmt"
                     /locus_tag="ACIE68_002420"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFX5908727.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21214..21723)
                     /gene="def"
                     /locus_tag="ACIE68_002421"
     CDS             complement(21214..21723)
                     /gene="def"
                     /locus_tag="ACIE68_002421"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFX5908728.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21853..22977
                     /gene="dprA"
                     /locus_tag="ACIE68_002422"
     CDS             21853..22977
                     /gene="dprA"
                     /locus_tag="ACIE68_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFX5908729.1"
                     /translation="MVDTDIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VTEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22949..23422
                     /gene="smg"
                     /locus_tag="ACIE68_002423"
     CDS             22949..23422
                     /gene="smg"
                     /locus_tag="ACIE68_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFX5908730.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23451..23993
                     /gene="yrdD"
                     /locus_tag="ACIE68_002424"
     CDS             23451..23993
                     /gene="yrdD"
                     /locus_tag="ACIE68_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFX5908731.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCLACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23998..24570
                     /gene="tsaC"
                     /locus_tag="ACIE68_002425"
     CDS             23998..24570
                     /gene="tsaC"
                     /locus_tag="ACIE68_002425"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFX5908732.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24575..25393
                     /gene="aroE"
                     /locus_tag="ACIE68_002426"
     CDS             24575..25393
                     /gene="aroE"
                     /locus_tag="ACIE68_002426"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFX5908733.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALSMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKCNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25390..25647
                     /gene="yrdB"
                     /locus_tag="ACIE68_002427"
     CDS             25390..25647
                     /gene="yrdB"
                     /locus_tag="ACIE68_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFX5908734.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25623..26177)
                     /gene="yrdA"
                     /locus_tag="ACIE68_002428"
     CDS             complement(25623..26177)
                     /gene="yrdA"
                     /locus_tag="ACIE68_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFX5908735.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6726 a         6247 c         6921 g         6522 t
ORIGIN      
        1 aaacaggaag ttctatgtct acgagaagag aagttattct ttcctggttg tgtgagaaac
       61 gtcaaacctg gcgtctatgc tatttgttgg gtgaggctgg aagtggaaaa acctggctgg
      121 cgcagcaact gcaaaaagat aaacatcgcc gtgtgattac tttaagcctc gttgtttcct
      181 ggcaaggtaa ggccgcatgg atcgttaccg acgataacgc ggctgaacag ggctgccgtg
      241 acagcgcctg gacgcgagat gagatggcgg ggcaattact gcatgcgctt catcgaactg
      301 atagtcgctg ccctcttatt attattgaaa atgctcacct gaatcaccgc cgcatacttg
      361 atgatctcca acgcgcaata tctcttattc ctgacggtca atttttgctg atcggcagac
      421 ccgatcgaaa agtcgaacgt gattttaaaa aacagggcat tgaacttgtc tcgataggac
      481 gtctgacgga gcacgaactt aaggcaagca tccttgaagg tcagaatatt gaccaacccg
      541 atctcctttt aaccgccaga gttctgaaac ggatagcttt attatgtcgg ggcgatcgca
      601 gaaagctggc gcttgccggc gaaacaattc gcttattaca gcaagccgag cagaccagcg
      661 ttttcacagc aaaacaatgg cgaatgattt accgcatatt gggtgataac cgcccccgga
      721 aaatgcagct tgccgtggtg atgagtggaa ccattatcgc tcttacctgt ggttggctac
      781 tgctgtcctc ttttacagct acgttacccg ttcctgcgtg gctgataccc gtaacgccag
      841 tagttaaaca agatatgact aaggatattg ctcatgtggt gatgcgcgat agtgaggcgc
      901 taagcgtgtt atacggcgta tgggggtacg aggtcccagc tgattcggcc tggtgcgatc
      961 aggcagtaag agcggggctg gcatgtaaaa gtgggaatgc ttctttacaa acattggtag
     1021 atcagaattt gccctggatc gcatcgctta aagtcgggga caaaaaattg cctgttgtgg
     1081 tcgttcgcgt cggtgaggct agcgttgatg tcctcgtagg tcagcaaacg tggacgctga
     1141 cacataaatg gtttgagtcg gtatggacgg gtgattatct tctgttgtgg aaaatgtcgc
     1201 cggaagggga gagtaccatt acgcgtgata gcagcgagga agagattctc tggctggaaa
     1261 cgatgttaaa ccgcgcattg catatttcga cagaaccttc ggcggagtgg cgtcccttgt
     1321 tggtagaaaa aataaagcaa tttcaaaaaa gccaccactt gaaaactgac ggtgttgttg
     1381 gcttttcaac gttagtacat ctctggcagg tggcagggga gagtgcctac ttatatcggg
     1441 atgaagcaaa tatttccccg gaaacaacag tgaaggggaa atgacatgtt tgaattctat
     1501 atcgccgcgc gtgagcagaa agaaacaggt caccctggta tcttctcccg acaaaaacac
     1561 agcaccataa tatatgtaat ttgtttatta ctgatttgcc tgtggtttgc aggaatggtg
     1621 ctggttggtg ggtatgccag gcagctatgg gtgctttgga tagtaaaagc cgaagtcact
     1681 gtagaggctg aaacgcccgc ttttaaacaa tcaacacaac actatttctt caaaaaacag
     1741 ccactccccg tcgtcgaatc cgtcgaggag gaggatgacc caggcgtcgc ggttgagaat
     1801 gccccctctt cttctgaaga cgaagaaaat actgtggaag aaagcgacga gaaagccggt
     1861 ttaagagagc gtgtcaaaaa tgcactgaac gaacttgaga gataacccga aggctgttta
     1921 ctttactagc aatacgcttg cgttcggtgg ttaagtatgt ataatgcgcg ggcttgtcgt
     1981 agttgacagc aggttcaatc tgaacctcag ttagccgaat ttggctacct aacaatgctc
     2041 ccaatcgggg agctacgtaa gaacggttac actctcccat caatcgtaat gggtctgagg
     2101 agtaatcatt ttcgtttata aaataattgg agctctggtc tcatgcagaa ccaaagaatc
     2161 cgtatccgcc tgaaagcgtt tgatcatcgt ctgatcgatc aagcaaccgc ggaaatcgtc
     2221 gagactgcca agcgcactgg tgcgcaggtc cgtggtccga tcccgctgcc gacacgcaaa
     2281 gagcgcttca ctgttctgat ctccccgcac gtcaacaaag acgcgcgcga tcagtacgaa
     2341 atccgtactc acttgcgtct ggttgacatc gttgagccaa ccgagaaaac cgttgatgct
     2401 ctgatgcgtc tggatctggc tgccggtgta gacgtgcaga tcagcctggg ttaatcaggt
     2461 cattgagcga ttgagaggtt gaaacaatga ttggtttagt cggtaaaaaa gtgggtatga
     2521 cccgtatctt cacagaagac ggcgtttcta tcccagtaac cgtaatcgaa gttgaagcaa
     2581 accgcgttac tcaggttaaa gacctggcta acgatggcta ccgtgctatt caggtgacca
     2641 ccggtgctaa aaaagctaac cgtgtgacca agcctgaagc tggccacttc gctaaagctg
     2701 gcgtagaagc tggccgtggt ctgtgggaat tccgcctggc tgaaggcgaa gagttcactg
     2761 taggtcagag cattagcgtt gaactgtttg ctgacgttaa aaaagttgac gtaactggca
     2821 cctctaaagg taaaggtttc gcaggtaccg ttaagcgctg gaacttccgt acccaggacg
     2881 ctactcacgg taactccttg tctcaccgcg ttccgggttc tatcggtcag aaccagactc
     2941 cgggcaaagt gttcaaaggc aagaaaatgg caggtcagat gggtaacgaa cgtgtaaccg
     3001 ttcagagcct tgacgtagta cgcgttgacg ctgagcgcaa cctgctgctg gttaaaggtg
     3061 ctgtcccggg tgcaaccggt agcgacctga tcgttaaacc agctgtgaag gcgtaaggag
     3121 atagcaatgg aattagtatt gaaagacgcg cagagcgcgc tgactgtttc cgaaactacc
     3181 ttcggtcgtg atttcaacga agcgctggtt caccaggttg ttgttgctta tgcagctggt
     3241 gctcgtcagg gtactcgtgc tcagaagact cgtgctgaag taactggttc cggtaaaaaa
     3301 ccgtggcgcc agaaaggcac cggccgtgcg cgttctggtt ctatcaagag cccgatctgg
     3361 cgttctggtg gcgtgacctt tgctgctcgt ccgcaggacc acagtcaaaa agttaacaag
     3421 aagatgtacc gcggcgcgct gaaaagcatc ctgtccgaac tggtacgtca ggatcgtctg
     3481 atcgttgtcg agaagttctc tgtagaagcg ccgaaaacta agctgctggc acagaaactg
     3541 aaagacatgg ctctggaaga tgtgctgatc atcaccggtg agctggacga aaacctgttc
     3601 ctggctgcgc gcaacctgca caaggttgac gtacgcgatg caactggtat cgacccggtt
     3661 agcctgatcg ccttcgacaa agtcgtaatg actgctgatg ctgttaagca agttgaggag
     3721 atgctggcat gattcgtgaa gaacgtctgc tgaaggtgct gcgtgcaccg cacgtttctg
     3781 aaaaagcgtc tactgcgatg gaaaaatcca acaccatcgt actcaaagtt gctaaagacg
     3841 cgaccaaagc agaaatcaaa gctgctgtgc agaaactgtt tgaagtcgaa gtcgaagtcg
     3901 ttaacaccct ggtagttaaa gggaaagtta aacgtcacgg acagcgtatc ggtcgtcgta
     3961 gcgactggaa aaaagcttac gtcaccctga aagaaggcca gaatctggac ttcgttggcg
     4021 gcgctgagta agtcggagga gtaatacaat ggcagttgtt aaatgtaaac cgacatctcc
     4081 gggtcgtcgc cacgtagtta aagtggttaa ccctgagctg cacaagggca aaccttttgc
     4141 tccgttgctg gaaaaaaaca gcaaatccgg tggtcgtaac aacaatggcc gtatcaccac
     4201 tcgtcatatc ggtggtggcc acaagcaggc ttaccgtatt gttgacttca aacgcaacaa
     4261 agacggtatc ccggcagttg ttgaacgtct tgagtacgat ccgaaccgtt ccgcgaacat
     4321 cgcgctggtt ctgtacaaag acggtgaacg ccgttacatc ctggccccta aaggcctgaa
     4381 agctggcgac cagattcagt ctggcgttga tgctgcaatc aaaccaggta acaccctgcc
     4441 gatgcgcaac atcccggttg gttctactgt tcataacgta gaaatgaaac caggtaaagg
     4501 cggtcagctg gcacgttccg ctggtactta cgttcagatc gttgctcgtg atggtgctta
     4561 tgtcaccctg cgtctgcgtt ctggtgaaat gcgtaaagta gaagcagact gccgtgcaac
     4621 tctgggcgaa gttggcaatg ctgagcatat gctgcgcgtt ctgggtaaag caggtgctgc
     4681 acgctggcgt ggtgttcgtc cgaccgttcg cggtaccgcg atgaacccgg tagaccaccc
     4741 acatggtggt ggtgaaggtc gtaactttgg taagcacccg gtaactccgt ggggcgttca
     4801 gaccaaaggt aagaagaccc gcagcaacaa gcgtactgat aaattcatcg tacgtcgccg
     4861 tagcaaataa ttttagagga taagccatgc cacgttctct caagaaaggt ccttttattg
     4921 acctgcactt gctgaagaag gtagagaaag cggtggaaag cggagacaag aagcccctgc
     4981 gcacttggtc ccgtcgttca acgatctttc ctaacatgat cggtttgacc atcgctgtcc
     5041 ataatggtcg tcagcacgtt ccggtatttg taaccgacga aatggtcggt cacaaactgg
     5101 gtgaattcgc accgactcgt acttatcgcg gccacgctgc tgataaaaaa gcgaagaaga
     5161 aataaggtag gaggaagaga tggaaactat cgctaaacat cgccatgctc gttcttctgc
     5221 tcagaaggtt cgccttgttg ctgacctgat tcgcggtaag aaagtgtcgc aggctctgga
     5281 tattttgacc tacaccaaca agaaagcggc tgtactggtc aagaaagttc tggaatctgc
     5341 cattgctaac gctgaacaca acgatggcgc tgacattgac gatctgaaag ttacgaaaat
     5401 tttcgtagac gaaggcccga gcatgaagcg cattatgccg cgtgcaaaag gtcgtgcaga
     5461 tcgcatcctg aagcgcacca gccacatcac tgtggttgtg tccgatcgct gagactctgg
     5521 agactagcaa tgggtcagaa agtacatcct aatggtattc gcctgggtat tgtaaaacca
     5581 tggaactcta cctggtttgc gaacaccaaa gaattcgctg acaacctgga cagcgatttt
     5641 aaagtacgtc agtacctgac taaggaactg gctaaagcgt ccgtatctcg tatcgttatc
     5701 gagcgtccgg ctaagagcat ccgtgtaacc attcacactg ctcgcccggg tatcgttatc
     5761 ggtaaaaaag gtgaagacgt agaaaaactg cgtaaggtcg tagcggacat cgctggcgtt
     5821 cctgcacaga tcaacatcgc cgaagttcgt aagcctgaac tggacgcaaa actggttgct
     5881 gacagcatca cttctcagct ggaacgtcgc gttatgttcc gtcgtgctat gaagcgtgct
     5941 gtacagaacg caatgcgtct gggcgctaaa ggtattaaag ttgaagttag cggccgtctg
     6001 ggcggcgcgg aaatcgcacg taccgaatgg taccgcgaag gtcgcgtacc gctgcacact
     6061 ctgcgtgctg acatcgacta caacacctct gaagcgcaca ccacttacgg tgtaatcggc
     6121 gttaaagtgt ggatcttcaa aggcgagatc ctgggtggta tggctgctgt tgaacaaccg
     6181 gaaaaaccgg ctgctcagcc taaaaagcag cagcgtaaag gccgtaaata aggagcgtcg
     6241 ctgatgttac aaccaaagcg tacaaaattc cgtaaaatgc acaaaggccg taaccgcggt
     6301 ctggcgcagg gtacggatgt tagcttcggc agcttcggtc tgaaagctgt tggccgtggt
     6361 cgtctgactg cccgtcagat cgaagcagca cgtcgtgcta tgacccgtgc agttaagcgt
     6421 caaggtaaga tctggatccg tgtgttcccg gacaaaccga tcactgaaaa gccgctggca
     6481 gtgcgtatgg gtaaaggtaa aggtaacgtg gagtattggg ttgccttgat tcagccgggt
     6541 aaagtcctgt atgaaatgga cggtgttccg gaagagctgg cccgtgaagc attcaagctg
     6601 gcagcagcga aactgccgat taaaaccacc tttgtaacta agacggtgat gtaatgaaag
     6661 caaaagagct gcgtgagaag agcgttgaag agctgaacac cgagctgctg aacctgctgc
     6721 gtgagcagtt caacctgcgt atgcaggctg caagtggcca gctgcaacag tctcacctgt
     6781 tgaagcaagt gcgtcgcgat gtcgcacgcg ttaagacttt actgaacgag aaggcgggtg
     6841 cgtaatgacc gataaaatcc gtactctgca aggtcgcgtt gttagcgaca aaatggagaa
     6901 atccattgtt gttgctatcg aacgttttgt gaaacacccg atctacggta aattcatcaa
     6961 gcgtacgacc aaactgcacg tacatgacga gaacaacgaa tgcggtatcg gtgacgtggt
     7021 tgaaatccgc gaatgccgtc cgctgtccaa gactaaatcc tggacgctgg ttcgcgttgt
     7081 agagaaagcg gttctgtaat acagtacact ctctcgatac gaataaacgg ctcagaaatg
     7141 agccgtttat tttttctacc catatccttg aagcggtgtt ataatgccgc gccctcgata
     7201 tggggatttt taacgacctg attttcgggt ctcagtagta gttgacatta gcggagcact
     7261 aaaatgatcc aagaacagac tatgctgaac gtcgccgaca actccggtgc acgtcgcgta
     7321 atgtgtatca aggttctggg tggctcgcac cgtcgctacg caggcgtagg cgacatcatc
     7381 aagatcacca tcaaagaagc aattccgcgt ggtaaggtca aaaaaggtga tgtgctgaag
     7441 gcggtagtgg tgcgcaccaa gaagggtgtt cgtcgcccgg acggttctgt cattcgcttc
     7501 gatggtaatg cttgtgttct tctgaacaac aacagcgagc agcctatcgg tacgcgtatt
     7561 tttgggccgg taactcgtga gcttcgtagt gagaagttca tgaaaattat ctctctggca
     7621 ccagaagtac tctaaggagc gaatcatggc agcgaaaatc cgtcgtgatg acgaagttat
     7681 cgtgttaacc ggtaaagata aaggtaaacg cggtaaagtt aagaatgtcc tgtcttccgg
     7741 caaggtcatt gttgaaggta tcaacctggt taagaaacat cagaagccgg ttccggccct
     7801 gaaccaaccg ggtggcatcg ttgaaaaaga agccgctatt caggtttcca acgtagcaat
     7861 cttcaatgcg gcaaccggca aggctgaccg tgtaggcttt agattcgaag acggcaaaaa
     7921 agtccgtttc ttcaagtcta acagcgaaac tatcaagtaa tttggagtag tacgatggcg
     7981 aaactgcatg attactacaa agacgaagta gttaaaaaac tcatgactga gtttaactac
     8041 aattctgtca tgcaagtccc tcgggtcgag aagatcaccc tgaacatggg tgttggtgaa
     8101 gcgatcgctg acaaaaaact gctggataac gcagcagcag acctggcagc aatctccggt
     8161 caaaaaccgc tgatcaccaa agcacgcaaa tctgttgcag gcttcaaaat ccgtcagggc
     8221 tatccgatcg gctgtaaagt aactctgcgt ggcgaacgca tgtgggagtt ctttgagcgc
     8281 ctgatcacta ttgctgtacc tcgtatccgt gacttccgtg gcctgtccgc taagtctttc
     8341 gacggtcgtg gtaactacag catgggtgtc cgtgagcaga tcatcttccc agaaatcgac
     8401 tacgataaag tcgaccgcgt tcgtggtttg gatattacca ttaccactac tgcgaaatct
     8461 gacgaagaag gccgcgctct gctggctgcc tttgacttcc cgttccgcaa gtaaggtagg
     8521 gttactaaat ggctaagcaa tcaatgaaag cacgcgaagt aaaacgcgta gctttagctg
     8581 ataaatactt cgcgaaacgc gctgaactga aagcgatcat ctctgatgtg aacgcttccg
     8641 acgaagatcg ttggaacgct gttctcaagc tgcagactct gccgcgtgat tccagcccgt
     8701 ctcgtcagcg taaccgctgc cgtcaaacag gtcgtccgca tggtttcctg cggaagttcg
     8761 ggttgagccg tattaaggtc cgtgaagccg ctatgcgcgg tgaaatcccg ggtctgaaaa
     8821 aggctagctg gtaattgtca ccaattgaat cacgggagta aagacagatg agcatgcaag
     8881 atccgatcgc ggatatgctg acccgtatcc gtaacggtca ggccgcgaac aaagctgcgg
     8941 tcaccatgcc ttcctccaag ctgaaagtgg caatcgccaa cgtgctgaag gaagaaggtt
     9001 ttattgaaga ttttaaagtt gaaggcgaca ccaagcctga actggaactt actctgaagt
     9061 atttccaggg caaagctgtt gtagaaagca ttcagcgtgt cagccgccca ggtctgcgca
     9121 tctataaacg taaagatgag ctgccgaaag ttatggcggg tctgggtatc gcagttgttt
     9181 ctacctctaa aggtgttatg actgatcgtg cagcgcgcca ggctggtctt ggtggcgaaa
     9241 ttatctgcta cgtagcctaa tcggaggaaa aaatgtctcg tgttgctaaa gcaccggtcg
     9301 ttgttcctgc cggcgttgac gtaaaaatca acggtcaggt tattacgatc aaaggtaaaa
     9361 acggcgagct gactcgtact ctcaacgatg ctgttgaagt taaacatgca gataataccc
     9421 tgaccttcgg tccgcgtgat ggttacgcag acggttgggc acaggctggt accgcgcgtg
     9481 ccctgctgaa ctcaatggtt atcggtgtta ccgaaggctt cactaagaag ctgcagctgg
     9541 ttggtgtagg ttaccgtgca gcggttaaag gcaatgtgat taacctgtct ctgggtttct
     9601 ctcatcctgt tgaccatcag ctgcctgcgg gtatcactgc tgaatgtccg actcagactg
     9661 aaatcgtgct gaaaggcgct gataagcagg tgatcggcca ggttgcagcg gatctgcgcg
     9721 cctaccgtcg tcctgagcct tataaaggca agggtgttcg ttacgccgac gaagtcgtgc
     9781 gtaccaaaga ggctaagaag aagtaaggta acactatgga taagaaatct gctcgtatcc
     9841 gtcgtgcgac ccgcgcacgc cgcaagctcc aggagctggg cgcaactcgc ctggtggtac
     9901 atcgtacccc gcgtcacatt tacgcacagg taattgcacc gaacggttct gaagttctgg
     9961 tagctgcttc tactgtagaa aaagctatcg ctgaacaact gaagtacacc ggtaacaaag
    10021 acgcggctgc agctgtgggt aaagctgtcg ctgaacgcgc tctggaaaaa ggcatcaaag
    10081 atgtatcctt tgaccgttcc gggttccaat atcatggtcg tgtccaggca ctggcagatg
    10141 ctgcccgtga agctggcctt cagttctaag gtagaggtgt aagatggctc acatcgaaaa
    10201 acaagctggc gaactgcagg aaaagctgat cgcggtaaac cgcgtatcta aaaccgttaa
    10261 aggtggtcgt attttctcct tcacagctct gactgtagtt ggcgatggta acggtcgcgt
    10321 tggttttggt tacggtaaag cgcgtgaagt tccagcagcg atccagaaag cgatggaaaa
    10381 agcccgtcgc aatatgatta acgtcgcgct gaataacggc actctgcaac accctgttaa
    10441 aggtgttcac acgggttctc gcgtattcat gcagccggct tccgaaggta ccggtatcat
    10501 cgccggtggt gcaatgcgcg ccgttctgga agtcgctggg gttcataacg ttctggctaa
    10561 agcatatggt tccaccaacc cgatcaacgt ggttcgtgca actattgatg gcctggaaaa
    10621 tatgaattct ccagaaatgg tcgctgccaa gcgtggtaaa tccgttgaag aaattctggg
    10681 gaaataaacc atggcaaaga ctattaaaat tactcaaacc cgcagtgcaa tcggtcgtct
    10741 gccgaaacac aaggcaacgc tgcttggcct gggtctgcgt cgtattggtc acaccgtaga
    10801 gcgcgaggat actcctgcta ttcgcggtat gatcaacgcg gtttccttca tggttaaagt
    10861 tgaggagtaa gagatgcgtt taaatactct gtctccggcc gaaggctcca aaaaggcggg
    10921 taaacgcctg ggtcgtggta tcggttctgg cctcggtaaa accggtggtc gtggtcacaa
    10981 aggtcagaaa tctcgttctg gcggtggcgt acgtcgcggt ttcgagggtg gtcagatgcc
    11041 tctgtaccgt cgtctgccga aattcggctt cacttctcgt aaagcagcga ttacagccga
    11101 agttcgtctg tctgacctgg ctaaagtaga aggcggtgtt gtagacctga acacgctgaa
    11161 agcggctaac attatcggta tccagatcga gttcgcgaaa gtgatcctgg ctggcgaagt
    11221 aacgactccg gtaactgttc gtggcctgcg tgttactaaa ggcgctcgtg ctgctatcga
    11281 agctgctggc ggtaaaatcg aggaataagt agcagatggc taaacaaccg ggattagatt
    11341 ttcaaagtgc caaaggtggc ttaggcgagc tgaaacgcag actgctgttt gttatcggtg
    11401 cgctgattgt gttccgtatt ggctctttta ttccgatccc tggtattgat gccgctgtac
    11461 ttgccaaact gcttgagcaa cagcgaggca ccatcattga gatgtttaac atgttctctg
    11521 gtggtgctct cagccgtgct tctatctttg ctctggggat catgccgtat atttcggcgt
    11581 cgatcattat ccagctgctg acggtggttc acccaacgtt ggcagaaatt aagaaagaag
    11641 gggagtctgg tcgtcgtaag atcagccagt acacccgtta cggtactctg gtgctggcaa
    11701 tattccagtc gatcggtatt gctaccggtc tgccgaatat gcctggtatg caaggcctgg
    11761 tgattaaccc aggctttgca ttctacttca ccgctgttgt aagtctggtc acaggaacga
    11821 tgttcctgat gtggttgggc gaacagatta ctgaacgagg tatcggcaac ggtatttcaa
    11881 tcattatctt cgccggtatt gtcgcgggac tcccgccagc cattgcccat actatcgagc
    11941 aagcgcgtca aggcgacctg cacttcctcg tgttgctgtt ggttgcagta ttagtatttg
    12001 cagtgacgtt ctttgttgta tttgttgagc gtggtcaacg ccgcattgtg gtaaactacg
    12061 cgaaacgtca gcaaggtcgt cgtgtctatg ctgcacagag cacacattta ccgctgaaag
    12121 tgaatatggc gggggtaatc ccggcaatct tcgcttccag tattattctg ttcccggcga
    12181 ccatcgcgtc atggttcggg ggcggtactg gttggaactg gctgacaaca atttcgctgt
    12241 atttgcagcc tgggcaaccg ctttatgtgt tactctatgc gtctgcaatc atcttcttct
    12301 gtttcttcta cacggcgttg gttttcaacc cgcgtgaaac agcagataac ctgaagaagt
    12361 ccggtgcatt tgtaccagga attcgtccgg gagagcaaac ggcgaagtat atcgataaag
    12421 taatgacccg cctgaccctg gttggtgcgc tgtatattac ctttatctgc ctgatcccgg
    12481 agttcatgcg tgatgcaatg aaagtaccgt tctacttcgg tgggacctca ctgcttatcg
    12541 ttgttgtcgt gattatggac tttatggctc aagtgcaaac tctgatgatg tccagtcagt
    12601 atgagtctgc attgaagaag gcgaacctga aaggctacgg ccgataattg gtcgcccgag
    12661 aagttacgga gagtaaaaat gaaagttcgt gcttccgtca agaaattatg ccgtaactgc
    12721 aaaatcgtta agcgtgatgg tgtcatccgt gtgatttgca gtgccgagcc gaagcataaa
    12781 cagcgccaag gctgattttt tcgcatattt ttcttgcaaa gttgggttga gctggctaga
    12841 ttagccagcc aatcttttgt atgtctgtgc gtttccattt gagtatcctg aaaacgggct
    12901 tttcagcatg gaacgtacat attaaatagt aggagtgcat agtggcccgt atagcaggca
    12961 ttaacattcc tgatcataag catgccgtaa tcgcattaac ttcgatttat ggcgtcggca
    13021 agacccgttc taaagccatc ctggctgcag cgggtatcgc tgaagatgtt aagatcagtg
    13081 agctgtctga aggacaaatc gacacgctgc gtgacgaagt tgccaaattt gtcgttgaag
    13141 gtgatctgcg ccgtgaaatc agcatgagca tcaagcgcct gatggatctt ggttgctatc
    13201 gcggtttgcg tcatcgtcgt ggtctcccgg ttcgcggtca gcgtaccaag accaacgcac
    13261 gtacccgtaa gggtccgcgc aaaccgatca agaaataatc ggggtgattg aataatggca
    13321 aaggcaccaa ttcgtgcacg taaacgtgta agaaaacaag tctctgacgg cgtggctcat
    13381 atccatgctt ctttcaacaa caccatcgtg actatcactg atcgtcaggg taacgcgttg
    13441 ggttgggcaa cagccggtgg ttccggtttc cgtggttctc gcaaatccac tccgtttgca
    13501 gctcaggttg cagcagagcg ttgcgctgac gccgtgaaag aatacggcat caagaatctg
    13561 gaagttatgg ttaaaggtcc gggtccaggc cgcgaatcta ctattcgtgc tctgaacgcc
    13621 gcaggtttcc gcatcactaa cattactgat gtgactccga tccctcataa cggttgtcgt
    13681 ccgccgaaaa aacgtcgcgt ataacgcctc gttttccagg tttgttggag aaagaaaatg
    13741 gcaagatatt tgggtcctaa gctcaagctg agccgtcgtg agggcaccga cttattcctt
    13801 aagtctggcg ttcgcgcgat cgataccaag tgtaaaattg aacaagctcc tggccagcac
    13861 ggtgcgcgta aaccgcgtct gtctgactat ggtgtgcagt tgcgtgaaaa gcaaaaagtt
    13921 cgccgtatct atggtgtgct ggagcgtcag ttccgtaact actacaaaga agcagcacgt
    13981 ctgaaaggca acaccggtga aaacctgttg gctctgctgg aaggtcgtct ggacaacgtt
    14041 gtataccgta tgggcttcgg tgccactcgt gcagaagcac gtcagctggt tagccataaa
    14101 gcaattatgg taaacggtcg tgttgttaac atcgcttctt atcaggttag tccgaatgac
    14161 gttgtaagca ttcgtgagaa agcgaagaag cagtctcgcg tgaaagccgc tctggagctg
    14221 gctgagcagc gtgaaaagcc aacctggctg gaagttgatg ctggcaagat ggaaggtacg
    14281 tttaagcgta agccggagcg ttctgatctg tctgcggaca ttaacgaaca cctgatcgtc
    14341 gagctttact ccaagtaaag cttagtacca aagagaggac acaatgcagg gttctgtgac
    14401 agagtttcta aaaccgcgcc tggttgatat cgagcaagtg agttcgacgc acgccaaggt
    14461 gacccttgag cctttagagc gtggctttgg ccatactctg ggtaacgcac tgcgccgtat
    14521 tctgctctca tcgatgccgg gttgcgcggt gaccgaggtt gagattgatg gtgtactaca
    14581 tgagtacagc accaaagaag gcgttcagga agatatcctg gaaatcctgc tcaacctgaa
    14641 agggctggcg gtgagagttc agggcaaaga tgaagttatt cttaccttga ataaatctgg
    14701 cattggccct gtgactgcag ccgatatcac ccacgacggt gatgtcgaaa tcgtcaagcc
    14761 gcagcacgtg atctgccacc tgaccgatga gaacgcgtct attagcatgc gtatcaaagt
    14821 tcagcgcggt cgtggttatg tgccggcttc tacccgaatt cattcggaag aagatgagcg
    14881 cccaatcggc cgtctgctgg tcgacgcatg ctacagccct gtggagcgta ttgcctacaa
    14941 tgttgaagca gcgcgtgtag aacagcgtac cgacctggac aagctggtca tcgaaatgga
    15001 aaccaacggc acaatcgatc ctgaagaggc gattcgtcgt gcggcaacca ttctggctga
    15061 acaactggaa gctttcgttg acttacgtga tgtacgtcag cctgaagtga aagaagagaa
    15121 accagagttc gatccgatcc tgctgcgccc tgttgacgat ctggaattga ctgtccgctc
    15181 tgctaactgc cttaaagcag aagctatcca ctatatcggt gatctggtac agcgtaccga
    15241 ggttgagctc cttaaaacgc ctaaccttgg taaaaaatct cttactgaga ttaaagacgt
    15301 gctggcttcc cgtggactgt ctctgggcat gcgcctggaa aactggccac cggcaagcat
    15361 cgctgacgag taaccggatc acaggttaag gttttactga gaaggataag gtcatgcgcc
    15421 atcgtaagag tggtcgtcaa ctgaaccgca acagcagcca tcgccaggct atgttccgca
    15481 atatggcagg ttcactggtt cgtcatgaaa tcatcaagac gactctgcct aaagcgaaag
    15541 agctgcgccg cgtagttgag ccgctgatta ctcttgccaa gactgatagc gttgctaatc
    15601 gtcgtctggc attcgcccgt actcgtgata acgagatcgt ggcaaaactg tttaacgaac
    15661 tgggcccgcg tttcgcgagc cgtgccggtg gttacactcg tattctgaag tgtggcttcc
    15721 gtgcaggcga caacgcgccg atggcttaca tcgagctggt tgatcgttca gagaaagcag
    15781 aagctgctgc agagtaatct gaagcaacgt aaaaaaaccc gccccggcgg gtttttttat
    15841 acccgtagca tccccactta tctacaatag ctgtactctt tttgttcatc ccctggagta
    15901 tttatgtggt tacttgacca gtgggcagag cgtcatatag cagaagcgca agcgaaaggt
    15961 gagtttgata acctggcagg tagcggcgaa ccattgatac tggatgatga ttctcacgtg
    16021 ccaccggaat tacgtgcggg gtatcgcttg ctgaagaatg ccggttgctt accgccagaa
    16081 cttgagcaac ggagagaagc aattcagctt ctggatatcc tgagaggtat ccgtcacgat
    16141 gatccgcaat atcaagaggt tagccgtcga ttgtcattac tggaattgaa gctgcgacaa
    16201 gctggattga gtaccgattt tttacgcggc gattatgctg acaagttgtt ggacaaaatc
    16261 aacgataact agtggagtat gtatgtatcg cattggtgag ctggcaaaaa tggcggaagt
    16321 aacacctgac acgattcgtt attacgaaaa acagcagatg atggagcatg aagtgcgtac
    16381 tgaaggcggg tttcgcctgt ataccgaaag cgatctccag cgattgaaat ttatccgcca
    16441 tgccagacaa ctaggtttca gtctggagtc gatccgcgag ttgctgtcga tccgcatcga
    16501 tcctgaacac catacctgtc aggagtcaaa aggcattgtg caggaaagat tgcaggaagt
    16561 cgaagcacgg atagccgagt tgcagagtat gcagcgttcc ttgcaacgcc ttaacgatgc
    16621 ctgttgtggg accgcccata gcagtgttta ttgttcgatt cttgaagctc ttgaacaagg
    16681 ggcgagtggc gttaagagtg gctgttgatt ttttgcactg gcaggattac actcgcgccg
    16741 taaaataacc aattggagtt tttatgagcc gatatcagca tactaaaggg cagataaagg
    16801 ataatgcgat agaagcatta ctacatgatc ccttattccg acagcgcgta gagaaaaata
    16861 agaaggggaa aggcagttac atgcgaaaag gaaaacatgg caatcggggg aactgggagg
    16921 ccagtggcaa gaaagtaaat cactttttta ccactggcct tctgctttca ggcgtttgtt
    16981 aagagcggtt attctgctct ttcagcaaat cacgaatttc tgtcagtaat acttcttctt
    17041 tagttggtgc tggtgcggct gctggttctt cttttttccg attcagtttg ttgattagct
    17101 taatcgccat aaagatggca aaggccacaa tcagaaaatc aaagacgttt tgaatgaaga
    17161 caccgtaatg catcacaaca gcagggatat ccccctgcgc atcgcgtagc gtgacagcaa
    17221 actgtttaaa gtcgatcccg ccaattaata agcccagagg tggcatgatg atatcggcaa
    17281 ccagtgaaga tacaatcttc ccgaatgccg caccgataat gacacccacc gccaaatcca
    17341 ccacgttccc gcgcatcgca aattcgcgaa attctttaat aatgctcatg ttattctccc
    17401 tatgaagccg acaaccataa gtctaacaaa tgttaagcca ttttcctgcc aggaatgaat
    17461 ttaattaatc cttgataatc aaagggaaat aataaggcgt cattagacgc cttattaatt
    17521 acaagaagaa agggcttggc tggaagagtc tttcgacgtc ggtaataaac tttttatctg
    17581 tgaggaacat aattacgtga tcgccttgct caatgcgcag attgtcattg gcaatcatca
    17641 cgtcgtttcc acgtaccacc gctccaataa tcgttcctgg cggtagcttg atttcgtcaa
    17701 tgactctgcc gacaacgcgt gaggtgcttt catcaccgtg agcaacggct tcaatagctt
    17761 ctgctacgcc gcggcgcaat gaggaaacac caacaatatc tgcttttcgc acatggctaa
    17821 gcaacgcaga aatagttgct tgttgtggtg aaatcgcaat atcgatcacg ctcccctgaa
    17881 ccagatccac ataagcgcga cgctggatca ataccatcac ctttttcgca cccatacgtt
    17941 tggcaagcat ggcggacatg atattggcct cgtcatcgtt ggtgacggca ataaacagat
    18001 caacttgatc gatatgttct tcggccagca gttcttgatc cgacgcatca ccaaaaaaga
    18061 cgatcgtatt ctgcaacttt tccgccagtt cggcagcgcg ctgctgatta cgttcgatga
    18121 gtttaacgct gtaatctttt tccagacgac gcgccagccc tgcaccgata ttaccgccac
    18181 caaccagcat gatccgctta tacggttttt ccagtcgctg taattcactc atcaccgcac
    18241 ggatatgctg tgaagcggca ataaagaaca cttcatcacc agcttccaca atggtcgaac
    18301 cttgtggacg aatggggcga tcgtggcgga aaattgctgc cacacgagta tcgatatgtg
    18361 gcatatgttc gcgcatggtc gaaagtgcgt taccaatcag cgggccgcca taataggctt
    18421 taaccacagc gaggctgact ttaccctcag cgaagttcac cacctgcaat gcgccgggat
    18481 actcaatcag tcggtaaata ttatcgataa ccaactgctc tggtgcgatc agatgatcaa
    18541 tcggtacagc atctgaatga aatagcttat cggcatcgcg cacgtagtct ggtgagcgga
    18601 tgcgagcgat gcgattaggg gtgttgaaaa gtgagtaggc tacctggcag gcaaccatat
    18661 tggtttcatc tgaactggtt acagcaacca gcatatcggc gtcgtcggca cctgcctccc
    18721 gcaatacgcg tggatgagag ccatgcccct gcacgactcg caggtcaaat ttatcctgca
    18781 aggtccgcag acgctcaccg ttggtatcga caacagtgat atcgttgttc tcgccaacca
    18841 ggttttccgc cagtgtgccg ccaacctggc cggcacccag aatgataatt ttcatcagtc
    18901 gcgacccgtt atctcatcac tttttgatta gcttagcgta aaagaagccg tcgccctctt
    18961 cggtaccagg cagattttgt ttacccggtt gctctggtgt tcctgtttcg caaagttcgg
    19021 catcagcggt acgttgcaaa aaggctttaa tctgcaggct attctcttcc ggtaacaccg
    19081 aacaggtggc atagaccagg gttccaccgg attttaaatg cggccaaatg gcgtcgagaa
    19141 tttcagactg caattgcgcg agttccggga tatcgcgatc gcgacgtaac catttaatat
    19201 ccggatggcg acgaatcaca ccggttgctg aacaaggcgc atctaataaa atgcgatcaa
    19261 actgttgctc gccacaccat tgggaagggt agcggccatc accttgtttc acggtcgctt
    19321 tcataccaag gcgttttaaa ttgtcgtaaa cgcgagagag gcgctgttcg tcaatatcaa
    19381 ccgcaacaac ctgcgcttct ggtgccacct caaggatatg cgttgtttta ccgccggggg
    19441 cggcacaaag atccagaatg tgttcaccgt tttgtggcgc aagccaggtc atgcaacctt
    19501 gtgctgatgc atcctgaacg gtaacccatc cgtcttcaaa accaggtagc gcatgaacag
    19561 gtgcaggtgt ttccagccgt acagcatcag ggtaatccgc atgcgggaaa cctttcattc
    19621 ctgcttcatc caacaatgca agccagctgt cgcgggaatg atgcgtacga ttaacgcgca
    19681 gccacattgg cggacgctgg ttattggctt cgacgatgga ttgccactgc tctggatacg
    19741 ctttttgcag acgcttcagc aaccaggaag gatgcagata acgtgcatca ctggcattaa
    19801 actcggctaa taactcttct tgctgacgct ggaactggcg taatacaccg tttatcaacc
    19861 ctttaagttg cggacgctta attgcgatag cgccttcaac cgtttcagcc agcgcagcat
    19921 gaggtggaat gcgggtataa agcagttgat acaaaccaac cataatcagg taatgcacgg
    19981 tccgctgttt gccggtcatc ggacgggcca ttaacttatt aatcagccag tctaactgcg
    20041 aaagcgtacg cagtacgcca aagcacaact cttgaagaag tgctttgtct ttatcggaaa
    20101 ctttttgctg gagcggtggc agaatgttgc ttaatgattg cccttgctcg acgacttgtt
    20161 caacggcctg ggccgccatg ctacgtaaat tacgttgttt tttcataagt ataaaaatgc
    20221 ccggcaagac cgggcttaga agagtggact atcagaccag acggttgccc ggaacaaacc
    20281 attcccgacg agagttcagg aggtcttgcg cgctcatcgc tttcttaccc gcaggttgta
    20341 acgagagcag gttcaggatg ccatcaccag tcgcaacctg aatgccttgt ttattggctt
    20401 caaggatcgt tcctggtgca gcgttggttg ccgtatcaat gaccgacgct ttccagactt
    20461 taaccggttg tccttcaatt tccagccagc tcattggcca tgggttgaaa gcgcgaatgc
    20521 agcgttcaag ctgcgctgcc gaaagcgacc agtcaatacg cgcttcttct ttactcaact
    20581 tctcggcgta agtgacaaga gtttcgtcct gaacttctgg tttcgccgtg ccgtctgcca
    20641 gttgtttcaa cgtggtgata agcccttgtg ggccaagctc tgccagcttg tcgtacagcg
    20701 taccactggt atcttctgca gtaatcgggc aggagagctt atagagcata tcaccggtgt
    20761 ctaaaccgac atccatttgc ataatggtca caccagtttc tgcatcaccc gcccatagtg
    20821 agcgttggat tggtgcagca ccgcgccagc gtggcagcag tgaaccatga acgttgatac
    20881 agccaagacg cggcatctcc agcactgctt taggcagaat taagccatag gcgacgacga
    20941 ccataacatc agcctgcaga tcggcgacca gttgctggtt ttcttgtgga cgcagggaaa
    21001 caggttgaaa aacgggcaga cctttttcct cagccagaac tttaaccggg ctgggcatca
    21061 gttttttacc gcgtcctgcc ggtcggtctg gctgggtgaa cacgccaacg acgttatgac
    21121 cagaagacaa cagcgcgtcg agatgacgcg ctgcaaagtc aggtgtaccc gcaaaaataa
    21181 tacgtagtga ttctgacacg ttagttctta tccttaagcc cgggctttca gacgatccag
    21241 tttttcaact ttctgacgaa tacgttgttg tttcaacggt gacagataat ccataaacag
    21301 tttgccgacc aggtgatcca tctcatgctg aatacagatg gctaacagac cgtctgcttc
    21361 cagttcaaat ggtttaccgt cgcggtccag ggcgcgaatt ttaactttct ctgcgcgtgg
    21421 cactaaagca cgttgttcag ggatcgacag gcaaccttct tcaatgcctg tttcgccgct
    21481 tttttctaaa agctccggat tgattaacac cagccgttcg tcacggtttt ccgaaacatc
    21541 aataacaatg atacgttgat ggatatcaac ctgggttgcc gccaggccaa taccttcttc
    21601 tgcgtacatc gtctcgaaca tatcatcgac gatacgctga atttctgcat tcacttcttc
    21661 taccggttta gcaactttgc gaagccgctc gtccggaata tgtaacactt gcaaaactga
    21721 cataaatctc cagagatgtg ttcaggagtt agaaagatta tttcttctat tctagacaaa
    21781 tccccctctg attgacagca tcactgacca atcgcaaaga ttgctaaggc tgcttatggc
    21841 agggagataa ggatggtcga tacagatatt tggctgcgtt taatgagcat cagcagcttg
    21901 tacggcgatg atatggtccg tatagctcac tggctggcaa aacagtcgca tattgatgcg
    21961 gttgtattgc agcaaacagg gcttacattg cggcaggcac aacgctttct ttcatttccg
    22021 cgaaagagta tcgaaagctc actttgttgg ttggagcaac ccaaccatca tttaatccct
    22081 gcggacagcg aattttatcc tcctcaactt ctggtgacga cagattaccc cggcgcactg
    22141 tttgttgaag gagaactgca cgcgctgcat tcatttcagc ttgccgtagt ggggagtcgg
    22201 gcgcattcat ggtatggcga gcgatgggga cggttatttt gcgaaactct ggcgacgcgt
    22261 ggagtgacaa ttacgagtgg actggcgcgt ggaatcgatg gtgtggcgca taaagctgcc
    22321 ttacaggtaa atggcgtcag cattgctgta ttggggaatg gacttaatac cattcatccc
    22381 cgccgccatg cccgactggc tgccagtctg cttgaacatg gcggagctct cgtctcggaa
    22441 tttcccctcg atgttccacc ccttgcttac aatttcccac gaagaaatcg cattatcagt
    22501 ggtctaagta aaggtgtact ggtggtggaa gcggctttgc gcagtggttc gctggtgaca
    22561 gcacgttgtg cgcttgagca ggggcgtgaa gtttttgcct tgccaggtcc aatagggaat
    22621 ccgggaagcg aagggcctca ctggttaata aaacaaggtg cgattcttgt gacggaaccg
    22681 gaagaaattc tggaaaactt gcaatttgga ttgcactggt tgccagacgc ccctgaaaat
    22741 tcattttatt caccagatca gcaagacgtg gcattgccat ttcctgagct cctggctaac
    22801 gtaggagatg aggtaacacc tgttgacgtc gtcgctgaac gtgccggcca acctgtgaca
    22861 gaggtagtta ctcaactact cgaactggag ttagcaggat ggatcgcagc tgtacccggc
    22921 ggctatgtcc gattgaggag ggcatgccat gttcgacgta ctaatgtatt tgtttgaaac
    22981 ctatattcac acagaagctg agttgcgtgt ggatcaagac aaacttgaac aggatcttac
    23041 cgacgcagga tttgatcgag aagatatcta caatgccctg ctttggctgg aaaagcttgc
    23101 ggattatcag gaaggactgg cagaaccgat gcaactggcc tctgatccac tctccatgcg
    23161 tatttatacc cctgaggagt gtgaaagact ggatgccagc tgtcgtggtt ttctgctttt
    23221 ccttgagcaa attcaggtgc ttaaccttga aacccgtgaa atggtgatag agcgagtgct
    23281 cgcgctggat accgcagagt tcgacctgga agacctgaaa tgggtaatcc tgatggtgtt
    23341 gttcaatatt ccgggctgcg aaaatgcgta ccagcaaatg gaagaattac tctttgaagt
    23401 gaatgaaggt atgctgcatt aatcatttct ttaattcagc ataagttgtt atggcgaaat
    23461 cagcactgtt cacggtgcgt aataatgagt cctgcccaaa gtgcggggct gaactggtta
    23521 ttcgatccgg gaaacacggt ccgtttcttg gatgctcaca gtatccggcg tgtgactacg
    23581 tccgtcctct gaaatcttca gcggatggac atattgtcaa agttctggag gggcaggttt
    23641 gccttgcatg tggcgcaaat ctggtattac gccagggacg ctttggtatg tttattggtt
    23701 gcagtaacta ccctgaatgc gaacataccg aacttatcga taaaccggac gaaacagcaa
    23761 ttacatgccc ccaatgccgg acgggccatc tggtccagcg ccgctcccgt tatggcaaaa
    23821 catttcactc ctgtgatcgc tacccggagt gtcaatttgc cattaacttc aaacccatag
    23881 ctggagaatg ccctgagtgt cattatccac tactcatcga aaagaaaacc gcgcagggtg
    23941 taaaacactt ttgtgccagt aaacaatgtg gaaagccggt ttcggcggaa taataacgtg
    24001 aataataacc tgcaaggaga cgctatcgca gctgcgatag atgtcctcaa tgaagaacgt
    24061 gtcatcgcct atccaacgga agccgttttc ggtgttgggt gcgatcctga tagcgaaaca
    24121 gcagtgatga gactgttgga gttaaaacag cgtccggttg ataaggggct gattttaatc
    24181 gcggcaaatt acgagcagct taaaccctat attgatgaca ccatgctgac tgatgcgcag
    24241 cgtgaaacca ttttttcccg ctggccaggt cctgtcacct ttgtctttcc cgcgcctgcg
    24301 acaacaccgc gctggttgac gggccgcttt gattcgcttg ctgtacgagt caccgaccat
    24361 ccgttggtgg ttgctttgtg ccaggcttat ggtaaaccgc tggtttctac cagtgccaac
    24421 ttgagtggat tgccaccttg tcgaacagta gacgaagttc gcgcacaatt tggcgcggcg
    24481 ttcccggttg tgcctggtga aacggggggg cgtttaaatc cttcagaaat ccgcgatgcc
    24541 ctgacgggtg aactgtttcg acaggggtaa cataatggaa acctatgctg tttttggtaa
    24601 tccgatagcc cacagcaaat cgccattcat tcatcagcaa tttgctcaac aactgaatat
    24661 tgaacatccc tatgggcgcg tgttggcacc catcaatgat ttcatcaaca cactgaacgc
    24721 tttctttagt gctggtggta aaggtgcgaa tgtgacggta ccttttaaag aagaggcttt
    24781 tgccagagcg gatgagctta ctgaacgggc agcgttggct ggtgctgtta ataccctcaa
    24841 gcggttagaa gatggacgct tgcagggtga caataccgat ggtataggtt tgttaagcga
    24901 tctggaacgt ctgtctttta tccgccctgg tttacgtatt ctgcttatcg gcgctggtgg
    24961 agcatctcgc ggcgtactac tgccacttct ttccctggac tgtgcggtga caatcactaa
    25021 tcggacggta tcccgcgcgg aagagttggc taaattgttt gcgcacactg gtagtattca
    25081 ggcgttgagt atggacgaac tggaaggaca tgagtttgat ctcattatta atgcaacatc
    25141 cagtggcatc agtggtgata ttccggcgat cccgtcatcg ctcattcatc caggcattta
    25201 ttgctatgac atgttctatc agaaaggaaa aactcctttt ctggcatggt gtgagcagcg
    25261 aggctcaaag tgtaatgctg atggtttagg aatgctggtg gcacaggcgg ctcatgcctt
    25321 tcttctctgg cacggtgttc tgcctgacgt agaaccagtt ataaagcaat tgcaggagga
    25381 attgtccgcg tgaatcaggc catccagttt ccggacagag aagagtggga cgagaataaa
    25441 aaatgtgtat gttttcccgc tctcgtgaat ggcatgcaac tgacatgtgc gatctctggc
    25501 gagagtctgg cgtatcgttt tactggagat acgccagaac agtggttagc gagttttcgt
    25561 cagcatcgct gggatctgga agaagaagcg gaaaacttaa ttcaggaaca aagtgaagat
    25621 gatcaaggct gggtctggtt accctgatcc agatattcgt ccttccattt cacgtaatta
    25681 ttcgcggaat agcgtaaccc agccttctct tcatcactta acgggcggat ctgtttgacg
    25741 gggctaccga gatacagata tccgctctcc agccgtttat tttgtgggac cagactaccc
    25801 gcaccaatca tcacatcatc ttctactatt gcgccatcaa gtaaaattga gcccatccca
    25861 accaaaactc gattgccaat ggtgcagccg tggagcatca ccttgtgacc aacagtgaca
    25921 tcttcgccaa tggttaatgg gttgccatct gggttgtacg aggatttatg agtgacatgc
    25981 aacatactgc catcctggat attggtgcgt gctccgatct gtacataatg tacatctcca
    26041 cgaatcacaa cgagcggcca gatccccaca tcatcagcca gacgaacgtc accaatcacg
    26101 acactgctat cgtcgatcat tacgcgctga ccgatttgtg gaaaaagatc gcggtatggg
    26161 cgtaaaacat cagacatact tacctcagca ataaatgatt tactaatgac tttgggggca
    26221 ttattggcct tgtgcaagtc ttttagtatg caaaaaagca ccgttttgtg tgcgattgca
    26281 gcaaaaaggg tgaaaaaaca acaaacagaa aaaaagatca aaaaaatact tgtgcaaaaa
    26341 attgggatcc ctataatgcg cctccgttga gacgacaacg tgaaacactt cacaagatgg
    26401 tcgtaacaac gaagag
//
LOCUS       DBANUU010000059        26008 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000059 DBANUU010000000
VERSION     DBANUU010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.059"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            441..1580
                     /gene="wza"
                     /locus_tag="ACIE68_002429"
     CDS             441..1580
                     /gene="wza"
                     /locus_tag="ACIE68_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFX5908736.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1586..2029
                     /gene="wzb"
                     /locus_tag="ACIE68_002430"
     CDS             1586..2029
                     /gene="wzb"
                     /locus_tag="ACIE68_002430"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFX5908737.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            2032..4194
                     /gene="wzc"
                     /locus_tag="ACIE68_002431"
     CDS             2032..4194
                     /gene="wzc"
                     /locus_tag="ACIE68_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFX5908738.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4372..5211
                     /gene="wcaA"
                     /locus_tag="ACIE68_002432"
     CDS             4372..5211
                     /gene="wcaA"
                     /locus_tag="ACIE68_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFX5908739.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5214..5702
                     /gene="wcaB"
                     /locus_tag="ACIE68_002433"
     CDS             5214..5702
                     /gene="wcaB"
                     /locus_tag="ACIE68_002433"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFX5908740.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5699..6916
                     /gene="wcaC"
                     /locus_tag="ACIE68_002434"
     CDS             5699..6916
                     /gene="wcaC"
                     /locus_tag="ACIE68_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFX5908741.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPMVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQECP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGRPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            6891..8108
                     /gene="wcaD"
                     /locus_tag="ACIE68_002435"
     CDS             6891..8108
                     /gene="wcaD"
                     /locus_tag="ACIE68_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFX5908742.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8119..8865
                     /gene="wcaE"
                     /locus_tag="ACIE68_002436"
     CDS             8119..8865
                     /gene="wcaE"
                     /locus_tag="ACIE68_002436"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFX5908743.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNSIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8881..9429
                     /gene="wcaF"
                     /locus_tag="ACIE68_002437"
     CDS             8881..9429
                     /gene="wcaF"
                     /locus_tag="ACIE68_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFX5908744.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            9455..10576
                     /gene="gmd"
                     /locus_tag="ACIE68_002438"
     CDS             9455..10576
                     /gene="gmd"
                     /locus_tag="ACIE68_002438"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFX5908745.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10579..11544
                     /gene="fcl"
                     /locus_tag="ACIE68_002439"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10579..11544
                     /gene="fcl"
                     /locus_tag="ACIE68_002439"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFX5908746.1"
                     /translation="MSKQRVFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVDYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENRDRFRG"
     gene            11547..12026
                     /gene="gmm"
                     /locus_tag="ACIE68_002440"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11547..12026
                     /gene="gmm"
                     /locus_tag="ACIE68_002440"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFX5908747.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIAENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            12023..13246
                     /gene="wcaI"
                     /locus_tag="ACIE68_002441"
     CDS             12023..13246
                     /gene="wcaI"
                     /locus_tag="ACIE68_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFX5908748.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13249..14685
                     /gene="cpsB"
                     /locus_tag="ACIE68_002442"
     CDS             13249..14685
                     /gene="cpsB"
                     /locus_tag="ACIE68_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFX5908749.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPDSDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14878..16248
                     /gene="cpsG"
                     /locus_tag="ACIE68_002443"
                     /gene_synonym="manB"
     CDS             14878..16248
                     /gene="cpsG"
                     /locus_tag="ACIE68_002443"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFX5908750.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKEKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16303..17697
                     /gene="wcaJ"
                     /locus_tag="ACIE68_002444"
     CDS             16303..17697
                     /gene="wcaJ"
                     /locus_tag="ACIE68_002444"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFX5908751.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17699..19177
                     /gene="wzxC"
                     /locus_tag="ACIE68_002445"
     CDS             17699..19177
                     /gene="wzxC"
                     /locus_tag="ACIE68_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFX5908752.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19357..20637
                     /gene="wcaK"
                     /locus_tag="ACIE68_002446"
     CDS             19357..20637
                     /gene="wcaK"
                     /locus_tag="ACIE68_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFX5908753.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINSLNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLELMKRSNITTAKVEDGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEAK"
     gene            20634..21854
                     /gene="wcaL"
                     /locus_tag="ACIE68_002447"
     CDS             20634..21854
                     /gene="wcaL"
                     /locus_tag="ACIE68_002447"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFX5908754.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGMAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21865..23259
                     /gene="wcaM"
                     /locus_tag="ACIE68_002448"
     CDS             21865..23259
                     /gene="wcaM"
                     /locus_tag="ACIE68_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFX5908755.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVSAGLVCDNINTGIFIPAGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLIIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHLIERINCT
                     NGKINWGIGIGLAGSTYDNNYPENQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVEIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23417..24412
                     /locus_tag="ACIE68_002449"
     CDS             23417..24412
                     /locus_tag="ACIE68_002449"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFX5908756.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            24655..25548
                     /gene="galF"
                     /locus_tag="ACIE68_002450"
     CDS             24655..25548
                     /gene="galF"
                     /locus_tag="ACIE68_002450"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFX5908757.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVQY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            25872..>26008
                     /locus_tag="ACIE68_002451"
     CDS             25872..>26008
                     /locus_tag="ACIE68_002451"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HFX5908758.1"
                     /translation="MIGIILAAGVGSRLRPMTNSKPKCLVTVAGKPILDYQLNSYRLA
                     G"
BASE COUNT         6452 a         6429 c         7010 g         6117 t
ORIGIN      
        1 taaaaaccat attgaatgac acttaatata attcttaaaa atagccaatt gccgaattgt
       61 taccttgcct gctattccgt tagctgtaac acttcctcct gcattattgg agagccaata
      121 ttcaattacc ataatgtcac cttatattcg gaagttaatt tccgcaataa ccctttctca
      181 tcaacgattg tatgcagaga gggtttgaaa aattaagcta aatcagtgta ttggtagcta
      241 aaaagccagg ggcggtagcg tgtctggatg cctgaaagac cagtctgaat tattctgtca
      301 atagcctgcg gatgaaatca atttttttag gactgatgcc agttaaattt ttattcaatt
      361 aactgcaagg taaatgtgca tggcgacatt gctattaata gtgcacagga taattactct
      421 gccaaagtga taaataaaca atgatgaaat ccaaaatgaa attgatgcca ttattggtgt
      481 cagtaacctt gataagcggt tgcacagtac ttccgggcag caatatgtcg acgatgggca
      541 aagacgtcat caaacagcag gacgctgatt tcgatctcga caaaatggtg aatgtttatc
      601 cgttgacccc gcgcctgatt gatcaattac gtccacgacc caacgtagcg cgccccaata
      661 tgacgctgga aagtgagatc gcgaattacc agtatcgcgt cgggccgggc gacgttctga
      721 atgtcaccgt ctgggatcac ccggaactca ccacgccagc cggtcagtac cgcagctcca
      781 gtgacaccgg caactgggta cagcctgacg gcactatgtt ttacccgtat atcggcaagg
      841 ttcacgtagt cgggaaaacg ctcgctgaaa tccgcagtga tattaccggg cgcttagcga
      901 cgtacatcgc tgacccgcag gtggacgtta atatcgccgc cttccgctca caaaaggcct
      961 atatctccgg acaggtgaat aaatccggtc aacaggcgat caccaacgta ccactgacca
     1021 ttctcgacgc catcaacgcc gcaggtggcc tgaccgacac cgctgactgg cgcaacgtgg
     1081 tgctaacaca caatggtcgt gaagaacgca tttctttgca ggcgctgatg caaaacggcg
     1141 acctcaatca gaaccgactg ctttatcccg gcgatattct ctacgtgccg cgtaatgatg
     1201 atctgaaagt gtttgtgatg ggtgaagtga agaaacagag caccctgaaa atggacttta
     1261 gcggcatgac cctgactgaa gccctgggca atgccgaagg tatcgacatg accacctcca
     1321 acgccagcgg catctttgtc attcgtccgc tgaaaggcga gggcgggcgt aatggcaaga
     1381 ttgccaatat ctaccagctg gatatgtccg atgcgacgtc gctggtgatg gcgacagaat
     1441 tccgcctgca accttatgac gtggtgtatg tcaccaccgc cccggtttcc cgctggaacc
     1501 gtctgatcaa tcagttgctg ccaactatta gcggtgtccg ttacatgacg gatacagcca
     1561 gcgacattca taactggtaa tcgtcatgtt taacaacatc ttagttgtct gtgtcggcaa
     1621 tatttgccgt tccccgacgg cggaacgctt actgcaacgt tatcacccgg agctgaaagt
     1681 ggagtccgct ggactcggcg cgctggtcgg taagggcgct gatcctaccg ctatcagcgt
     1741 cgccgcagaa catcaactgt ctctggaagg tcactgtgca cggcaaatca gccgccgcct
     1801 gtgtcgcaac tatgacctga ttttgaccat ggaaaagcgc catatcgaac gcttatgcga
     1861 gatggcacct gagatgcgcg gcaaagtgat gctgtttggt cactgggata acgaatgtga
     1921 aatccccgat ccgtatcgca aaagccggga aacgtttgca gcggtgtaca cattacttga
     1981 acggtctgcc cgccagtggg cgcaggcatt gaacgcagag caggtataag aatgacagaa
     2041 aaagtaaaac aacatgccgc tccggtaacg ggcagtgatg aaatcgatat tggtcgcctg
     2101 gtcggcaccg tcattgaagc gcgctggtgg gtgattggca tcaccgctgt atttgccctc
     2161 tgtgctgtgg tttacacttt cttcgccacg ccgatttata gtgccgacgc actggtacaa
     2221 atcgaacaaa acagcggcaa ttcgttagtg caggacattg gctcggcatt agccaacaaa
     2281 ccgcctgcat cggacgccga gatccagttg attcgttcgc gtctggtgct tggtaaaacg
     2341 gtggacgatc tagacctcga tattgcggtg agcaaaaaca cgttcccgat tttcggtgcg
     2401 ggttgggatc gcctgatggg acgtcagaac gagacggtga aagtgaccac ctttaaccgc
     2461 ccgaaagaga tggcggacca ggtgtttacg cttaatgtgc tggatgacaa aaactacact
     2521 ctgagcagtg atggcggctt tagcgcccgt gggcaagcgg gccagatgct gaaaaaagaa
     2581 ggcgtcacgc tgatggttga agccattcac gcccgcccgg gcagtgagtt taccgtcacc
     2641 aaatactcca cgttggggat gatcaaccaa ctgcaaaaca gcctgacggt aacggagaac
     2701 ggcaaagacg caggcgtact gagcctgact tataccggtg aagatcgcga acagatccgc
     2761 gacattctta acagcatcgc ccgtaactat caggaacaaa atattgagcg caaatcggcg
     2821 gaagcgtcga aaagcctcgc tttcctcgcg caacagttac cggaagtacg tagccgcctc
     2881 gatgttgccg aaaacaaact gaatgccttc cgtcaggata aagattctgt tgatctgccg
     2941 ctggaagcga aagcggtgct cgattcgatg gtgaacatcg acgcgcagtt gaacgaactg
     3001 acctttaaag aggcggaaat ctccaagctg tacaccaaag ttcaccccgc gtaccgcacg
     3061 ctgctggaga aacgtcaggc gctggaagac gaaaaagcca aacttaatgg tcgcgtaacg
     3121 gcgatgccga aaacccagca ggaaattgtc cgtctgaccc gcgatgtcga gtctggtcag
     3181 caggtctata tgcaactgct gaataaagag caggagctga aaatcaccga agccagcacc
     3241 gtcggcgatg tgcgcattgt tgacccggca atcacccagc ctggcgtgct aaaaccgaag
     3301 aaagggctga ttatcctcgg cgcgattatc cttggcctga tgctctctat cgtgggtgtg
     3361 ctgctgcgct cgttgtttaa tcgcggcatc gaaagcccgc aagtgctgga agaacacgga
     3421 atcagcgtct atgccagcat cccgctgtcg gagtggcaga aagcgcgcga tagcgtcaaa
     3481 accatcaaag gggttaaacg ctataaacag agccagctac tggcagtggg gaatccaacc
     3541 gatctggcga ttgaagccat ccgtagtctg cgtaccagtc tgcacttcgc gatgatgcag
     3601 gcgcagaaca atgtgttgat gatgaccggg gttagcccgt caattggtaa aacctttgtc
     3661 tgcgccaacc tggcggcggt gatcagccag accaataaac gggtgttgtt gatcgactgt
     3721 gatatgcgca aaggctacac ccacgagctg ttgggcacca ataacgtcaa tggtctgtcg
     3781 gaaattctga ttggccaggg cgatattact accgcagcta aaccgacctc tattgccaaa
     3841 tttgacctga tcccgcgcgg tcaggtaccg ccaaatcctt ctgaactgtt gatgagcgaa
     3901 cgctttgccg aactggtgaa ctgggcgagt aaaaactatg acctggtgtt gattgatacg
     3961 ccgccgattc tggcagtgac cgatgcggca attgttggtc gtcatgtcgg aaccacgtta
     4021 atggtggcgc gttatgcggt caacacattg aaagaagtgg aaaccagtct gagccgcttt
     4081 gagcaaaacg gtattccggt gaaaggggtg attctgaact ccatcttccg ccgcgccagc
     4141 gcgtatcagg attatggcta ttacgaatac gaatataagt cggatgcgaa ataacgacat
     4201 ccgctgtcgg atgcgatgct gacgcatctt atccgaccta ccttcacgcc gcatccggca
     4261 agcaaaccag ctccgtaggc cggataaggc gctcacgccg catccggcaa cagaacccgt
     4321 aaacaagctt ataaaccggc agaacatctc gacactccgg gagaacaacc tatgaaagac
     4381 aatccgctga tttcaatcta tatgccgacc tggaaccgcc aacaactggc gattcgggcg
     4441 ataaaatcgg tcctgcgcca ggactacagc aactgggaga tgatcatcgt ggatgattgc
     4501 tccacctcct gggaacaact gcaacagtac gtcactgccc tcaacgatcc gcgtattacc
     4561 tacattcaca acgacattaa cagcggggcg tgcgcggtac gtaaccaggc gattatgctg
     4621 gcgcaagggg aatacattac cgggatcgat gacgatgatg aatggacgcc caaccgcctg
     4681 agcgtcttcc tcgcccataa acagcaactg gtcactcacg cctttttgta cgctaacgac
     4741 tacgtttgcc agggagaagt ttattcccaa ccggcaagct taccactgta tcccaaatcg
     4801 ccgtactcac gccgcctgtt ctataaacgc aatatcattg gcaatcaggt ctttacctgg
     4861 gcatggcgtt tcaaagagtg tttgttcgat accgaactga aagcagcaca ggattacgac
     4921 atcttcctgc ggatggtggt ggagtacggc gaaccgtgga aagtggaaga ggcgacgcag
     4981 atcctgcata tcaatcacgg cgaaatgcag atcacctcgt caccgaaaaa attctccgga
     5041 tacttccact tttaccgcaa gcacaaagac aaattcgacc gcgccagtaa aaaatatcag
     5101 ctgtttaccc tctaccagat ccgcaataaa cgtatgacct ggcgcacctt gttgacgctg
     5161 ctgtcggtgc gtaacggcaa acgtctggct gatggtattc gggggcgcta aagatgctgg
     5221 aagatctgcg cgccaacagc tggagtttac gcccgtgctg catggtactt gcctatcgcg
     5281 tcgctcattt ttgttcggtg tggcgcaaaa agaacgtcct caacaatctg tgggcggccc
     5341 cgctgctggt gctgtatcgc attatcaccg aatgcttttt cggttatgaa atccaggctg
     5401 ccgcgaccat tggccgccgc tttactatcc atcacggtta cgccgtcgtg atcaataaaa
     5461 acgtggtagc gggggatgat tttaccattc gccacggcgt cactatcggc aatcgtggtg
     5521 ccgataacat ggcatgtcca cacattggca acggcgtcga actcggtgcc aacgtcatta
     5581 ttcttggtga tatcacgctt ggtaacaacg tcaccgtggg cgcgggcagc gtggtgctcg
     5641 actctgtccc ggacaacgcg ctggtggtgg gagaaaaagc gcgagtgaag gtaattaaat
     5701 gaatattttg caatttaatg tgcgactggc ggaaggcggg gcagcaggtg tggcgttaga
     5761 tctccaccag cgtgcgctgc aacaggggct ggcgtcacat tttgtctacg gttacggcaa
     5821 aggcggcaaa gagagcgtca gccatcagaa ctatccgcag gtcatcaaac atacgccgcg
     5881 gatgaccgcg atggcgaata ttgccctgtt tcgtctgttt aatcgcgatc tgtttggcaa
     5941 tttcaatgag ttatatcgca ccattactcg cacaccgggt ccgatggtcc tgcattttca
     6001 tgtgctgcac agctactggc taaatcttaa gagcgtggtg cgcttttgcg aaaaagtgaa
     6061 aaaccataaa ccggacgtca ctctggtctg gacgctgcac gaccactgga gtgtaaccgg
     6121 acgctgcgcc tttaccgacg gttgcgaagg ctggaaaacg ggctgccagg aatgcccgac
     6181 cttaaataat tatccgccgg tgaagattga tcgcgcacac caactggtgg cgggcaaacg
     6241 ccagttattc cgcgagatgc tggcgctggg ctgtcagttt atttccccca gccagcatgt
     6301 ggctgatgct ttcaatagtc tgtacgggcc agggcgttgc cggattatca ataatggcat
     6361 tgatatggca accgaagcga ttctggcgga cttgcctccg gtgcgcgaaa cccagggcag
     6421 gccgaaaatc gcggtggtgg cgcatgacct gcgttacgac ggcaaaacta accagcaact
     6481 ggtgcgcgag atgatggcgc tgggcgacaa aattgaactg catacctttg gtaagttctc
     6541 gccgttcacc gctggcaacg tggttaatca cggctttgaa actgacaagc gcaagttgat
     6601 gagcgcgctc aatcagatgg atgcgctggt gttcagttct cgcgtcgata actacccgct
     6661 gattttgtgt gaggcgctat cgattggcgt gccggtgatt gccacccata gcgatgcggc
     6721 gcgggaagtg ttgcaaaaat ccggcggtaa aaccgtcagc gaagaagagg tgctgcaact
     6781 ggtgcagtta agcaaaccgg aaatcgcgca ggcgatattt ggtaccacgc tggctgagtt
     6841 cagccaacgc agccgcgcca cctacagtgg acaacagatg ctggaggagt atgtcaactt
     6901 ctatcagaat ctgtagctac ctgctgctgc cgctgatcta cctgctggtt aacgtcaaaa
     6961 tcgcccagct tggcgaaagt ttccccatca ccatcgtcac ttttttacct gtcttgttac
     7021 tgctgttttt agaacgcatc agcgttaaaa aattgatgat tgccttaggg attggtgcgg
     7081 gactcacggc gtttaactat ctgtttggtc agtcgctgga tgccagtaaa tacgtcactt
     7141 caaccatgct gtttgtctat attgtgatca ttattggcat ggtgtggagt attcgtttta
     7201 aaacgatttc gccacacaac catcgcaaga tattacgttt cttttatctg gtggtcgggc
     7261 tggtggtggc gctggcggca gtggagatgg cgcaaattat cctcaccggt ggcagcagta
     7321 ttatggagtc gatttcgaaa tatctgattt acagcaacag ctatgtgctg aatttcatta
     7381 aattcggcgg taagcgtacg acagcacttt atttcgaacc ggcatttttc gctctggcat
     7441 taatctcaat ttggctcagc atcaaacagt ttggtatcaa aacgcctaaa accgatgcta
     7501 tgattctcgc agggataata ttatccggat cgttttcagg ggttatgacc tttatcctgt
     7561 tttatttgct ggagtgggca tttcaatatc tgaataaaga ggcgattaag aaaaagttac
     7621 cgttagcatt gatttctctg gctgtattcc tggttggtgt ggtaatcgcg tttccttata
     7681 tttccacccg tctgggcgat ttaggtacgg aaggatcgtc atcgtattat cgtattgtcg
     7741 gtccgctggt gatggtcggt tattctttga cccatattga cggtgtagtc agatttggct
     7801 cactttatga atatgtcgca tcattcggaa tatttaacgg tgcggatgtc ggaaaaacca
     7861 tagacaatgg tttgtatctg ctgattattt atttttcctg gttcgcggtg tttttatcgc
     7921 tgtggtacat ggggaaagtg ataaaaatga tgatcaacgc ttttggtgat aaccgcaatt
     7981 ttcgcgtgca attatatctt tttactccgg tatcgctgtt ttttaccggt tcgatattta
     8041 gcccggaata tgcattttta atcgtctgtc cgtttatttt gcgaaaagcg ttaaatatta
     8101 cgaggtaaga ataagaacat gttgcttagc ataatcactg tcgcgtttcg taacctcgaa
     8161 gggatagtca aaacacatgc ctcgctggcg catctggcgc aggcggacga tatcagcttc
     8221 gaatggattg ttgtcgatgg cggttccaac gacggtaccc gtgagtatct ggaaaatctc
     8281 aatagtatct ataacctacg ctttgtcagc gagccagata acggtatcta cgacgccatg
     8341 aacaagggca ttgcgatggc acaaggcaag ttcgcgttgt ttctcaattc gggcgatatt
     8401 tttcatcagg atgccgcaaa ttttgtccgt aagttaaaaa tgcaaaaaga taacatgatg
     8461 atcaccggcg atgcgctgct ggattttggc gacgggcata aaattaaacg tagcgccaaa
     8521 ccgggctggt atatttatca cagcctgccc gccagccatc aggcgatatt tttcccggta
     8581 tccggtttga aaaaatggcg ttatgacctg gaatataaag tttcttctga ttacgcgctg
     8641 gcagctaaaa tgtataaagc aggttatgca tttaaaaaac tcaatggcct ggtgtctgaa
     8701 ttttccatgg gtggggtatc aaccaccaat aatatggaat tgtgtgctga cgccaaaaaa
     8761 gtccaacggc agatattaca tgtgcctggc ttctgggcag aattatcctg gcatttacgc
     8821 caacgtacta cctcaaagac gaaagcctta tataacaaaa gctgaatata agggaaaacg
     8881 atgcaggatt taagcggttt ctcggtgccg aaagggttcc ggggcggcaa cgctattaaa
     8941 gtgcaattat ggtgggcagt acaggcaaca atatttgcct ggtcgccaca agtattgtat
     9001 cgctggcggg cttttttatt acgtttattc ggagcaaaaa taggaaaaaa cgtagttatt
     9061 cgtccgtcag taaaaattac ctatccgtgg aaattaacct taggtgatta cgcgtgggtc
     9121 ggcgatgacg tcaatttata taccctcggt gaaataacca ttggcgcaca ttcggtgata
     9181 tcgcaaaaaa gttatttatg caccggtagc cacgaccatg caagtcaaca tttcaccatt
     9241 aacgccacgc ctattgtgat tggcgagaaa tgctggctgg caaccgatgt ctttgttgcc
     9301 ccaggcgtca caatcggcga cggcaccgtc gtgggtgcac gaagcagtgt ttttaaatcg
     9361 cttccggcaa atgtggtttg ccgggggaat cccgcagtgg tgatacgcga acgcgttgaa
     9421 actgaataaa ttcaaaaata cagaggaata agacatgtca aaagtcgctc tcatcaccgg
     9481 tgtaaccgga caagacggtt cttacctggc agagtttctg ctggaaaaag gttacgaggt
     9541 gcatggtatt aagcgtcgcg catcgtcatt caacaccgag cgcgtggatc acatttatca
     9601 ggatccgcac acctgcaacc cgaaattcca tctgcattat ggcgacctga gtgatacctc
     9661 taacctgacg cgcattttgc gtgaagtgca gccggatgaa gtgtacaacc tgggcgcaat
     9721 gagccacgtt gcggtctctt ttgagtcacc agaatatacc gctgacgtcg acgcgatggg
     9781 tacgctgcgc ctgctggagg cgatccgctt cctcggtctg gaaaagaaaa ctcgtttcta
     9841 tcaggcttcc acctctgaac tgtacggtct ggtgcaggaa attccgcaga aagaaaccac
     9901 gccgttctac ccgcgatctc cgtatgcggt cgccaaactg tacgcctact ggatcaccgt
     9961 taactaccgc gaatcctacg gcatgtacgc ctgtaacggt attctcttca accatgaatc
    10021 cccgcgccgc ggcgaaacct tcgttacccg caaaattacc cgcgcaatcg ccaacatcgc
    10081 ccaggggctg gagtcgtgcc tgtacctcgg caatatggac tccctgcgtg actggggcca
    10141 tgccaaagac tacgtaaaaa tgcagtggat gatgctgcaa caggaacaac cggaagattt
    10201 tgttatcgcg accggcgttc agtactccgt acgtcagttc gtggaaatgg cggcagcaca
    10261 gttgggcatc aaactgcgct ttgaaggcac gggtgttgaa gagaagggca ttgtggtttc
    10321 cgtcaccggg catgacgcgc cgggcgttaa accgggtgat gtgattatcg ctgttgaccc
    10381 gcgttacttc cgtccggctg aagttgaaac gctgctcggc gacccgacca aagcgcacga
    10441 aaaactgggc tggaaaccgg aaatcaccct cagagagatg gtgtctgaaa tggtggctaa
    10501 tgacctcgaa gcggcgaaaa aacactctct gctgaaatct cacggctacg acgtggcgat
    10561 cgcgctggag tcataagcat gagtaaacaa cgagttttta ttgctggtca tcgcgggatg
    10621 gtcggttccg ccatcaagcg acagctcgaa cagcgcggtg atgtggagct ggtattacgc
    10681 acccgcgatg aactgaacct gctggacagc cgcgcggtgc atgatttctt tgccagcgaa
    10741 agcattgacc aggtctatct ggcggcggcg aaagtgggcg gcattgttgc caacaacacc
    10801 tatccggcgg atttcatcta ccagaacatg atgattgaga gcaacatcat tcacgccgcg
    10861 catcagaacg acgtgaacaa actgctgttt ctcggatcgt cctgtatcta cccgaaactg
    10921 gcaaaacagc cgatggcaga aagcgagtta ttgcagggca cgctggagcc gaccaacgag
    10981 ccttatgcca ttgccaaaat tgccgggatc aaactgtgcg aatcttacaa ccgccagtac
    11041 ggacgcgatt accgctcagt catgccgacc aacctgtatg gcccgcatga caacttccac
    11101 ccgagtaatt cgcatgtgat cccagcattg ttgcgtcgct tccacgaggc gacggcacag
    11161 aatgcgccgg acgttgtggt atggggcagc ggtacaccga tgcgtgaatt cctgcacgtc
    11221 gatgatatgg cggcggccag cattcacgtg atggagctgg cgcacgaagt ctggctggag
    11281 aacacccagc cgatgctgtc gcacattaac gtcggtaccg gtgttgactg cactatccgc
    11341 gagctggcgc aaaccatcgc caaagtggtg gattacaaag gtcgggtggt ttttgatgcc
    11401 agcaagccgg atggtacgcc gcgcaaactg ctggatgtga cgcgcctgca tcagcttggc
    11461 tggtatcacg aaatctcact ggaagcgggg cttgccagca cttaccagtg gttccttgag
    11521 aatcgagacc gctttcgggg gtaatgatgt ttttacgtca ggaagacttt gccacggtag
    11581 tgcgctccac accgcttgtc tctctcgact ttattgccga gaacagtcgc ggcgagtttc
    11641 tgcttggcaa aagaaccaac cgcccggcgc agggttactg gtttgtgccg ggagggcgcg
    11701 tgcagaaaga cgaaacgctg gaagccgcat ttgagcggct gacgatggcg gaactggggc
    11761 tgcgtctgcc gataacagca ggccagtttt acggtgtctg gcagcacttt tatgacgata
    11821 acttctctgg cacggatttc accactcact atgtggtgct cggttttcgc ttcagagtcg
    11881 ctgaagaaga tctgatactg ccggatgagc agcatgacga ttaccgctgg ctgacgccgg
    11941 acgcgctgct cgccagtaac gatgttcacg ctaacagccg cgcctatttt ctcgctgaga
    12001 agcgtgccgg agtacccgga ttatgaaaat actggtctac ggcattaact actcgccgga
    12061 gttaaccggt atcggcaaat acaccggcga gatggtggaa tggctggcgg cgcaaggtca
    12121 tgaggtgcgg gttattaccg caccgcctta ctacccgcag tggcaggtgg gcgagaacta
    12181 ttccgcctgg cgctacaaac gagaagaggg ggccgccacg gtgtggcgct gcccgctgta
    12241 cgtgccaaaa cagccgagca ccctgaaacg cttgttgcat ctcggcagtt ttgccgtcag
    12301 cagtttcttt ccactgatgg cgcaacgtcg ctggaagccg gatcgcatta tcggcgtagt
    12361 gccaacgctg ttttgcacgc cgggaatgcg cctgctggcg aagctctctg gtgcgcgtac
    12421 cgtgctgcat attcaggatt acgaagtgga cgccatgctg gggctgggcc ttgccggaaa
    12481 aggcaaaggc ggcaaagtgg cacagctggc aacggcgttc gaacgtagcg gactgcataa
    12541 cgtcgataac gtctccacga tttcgcgttc aatgatgaat aaagccatcg aaaaaggcgt
    12601 ggcggcggaa aacgtcatct tcttccccaa ctggtcggaa attgcccgtt ttcagcatgt
    12661 tgcagatgcc gatgttgacg cccttcgtaa ccagcttgac ctgccggata acaaaaaaat
    12721 cattctttac tccggcaata ttggtgaaaa gcaggggctg gaaaacgtta ttgaagctgc
    12781 cgatcgtctg cgcgatgaac cgctgatttt tgccattgtc gggcagggcg gcggcaaagc
    12841 gcggctggaa aaaatggcgc agcagcgcgg actgcgcaat atgcaatttt tcccgctgca
    12901 atcgtatgac gctttacccg cactgctgaa gatgggcgat tgccatctgg tggtgcaaaa
    12961 acgcggcgcg gcagatgccg tattgccgtc gaaactgacc aatattctgg cggtaggcgg
    13021 taacgcggtg attaccgcag aggcccacac agaactgggg cagctttgcg aaacctttcc
    13081 gggcattgcg gtttgcgttg aaccggaatc ggttgaggcg ctggtggcgg ggattcgtca
    13141 ggcgctcctg ctgcccaaac acaacacggt ggcacgtgaa tatgccgaac gcacgctcga
    13201 taaagagaac gtgttacgtc aatttataaa tgatattcgg ggataattat ggcgcagtcg
    13261 aaactctatc cagttgtgat ggcaggtggc tccggtagcc gcttatggcc gctttcccgc
    13321 gtactttatc ccaagcagtt tttatgcctg aaaggcgatc tcaccatgct gcaaaccacc
    13381 atctgccgcc tgaacggcgt ggagtgcgaa agcccggtgg tgatttgcaa tgagcagcac
    13441 cgctttattg tcgcggaaca gctgcgccaa ctgaacaaac tcaccgagaa cattattctg
    13501 gaacctgcag ggcgtaacac tgcacctgcc attgcgttgg cggcgctggc ggcaaaacgt
    13561 catagtccgg acagcgatcc gttaatgttg gtcctggcgg cagatcatgt gattgccgac
    13621 gaagacgcgt tccgcgccgc cgtgcgtaat gccatgccat atgccgaagc gggcaagctg
    13681 gtcaccttcg gcattgtgcc ggatctacca gaaaccggtt atggctatat tcgtcgcggt
    13741 gaagtgtctg cgggcgagca ggatacggtg gcctttgaag tggcgcagtt tgtcgaaaaa
    13801 ccgaatctgg aaaccgccca ggcctatgtg gcaagcggtg aatattactg gaacagcggc
    13861 atgttcctgt tccgcgccgg acgctatctc gaagaactga aaaaatatcg cccggatatt
    13921 ctcgacgcct gcgaaaaagc gatgagcgcc gtcgatccgg atctcgattt tattcgtgtg
    13981 gatgaagagg cgtttcttgc ctgcccggaa gagtcggtgg attacgcggt catggaacgt
    14041 acggcagatg ccgttgtggt gccgatggat gcgggctgga gcgatgtcgg ttcctggtcc
    14101 tctttatggg agatcagcgc ccacaccgcg gaaggcaacg tttgccacgg cgatgtgatt
    14161 aatcacaaaa ctgaaaacag ctatgtgtat gccgaatctg gcctggtcac caccgtcggg
    14221 gtgaaagatc tggtagtggt gcagaccaaa gacgcggtgc tgattgcaga ccgtaacgcg
    14281 gtgcaggatg tgaaaaaagt ggtcgagcag atcaaagccg atggtcgcca tgagcatcgg
    14341 gtgcatcgcg aagtgtatcg cccgtggggc aaatatgact ctatcgacgc gggcgaccgc
    14401 taccaggtga aacgcatcac cgtgaaaccg ggcgagggct tgtcggtaca gatgcaccat
    14461 caccgcgcgg aacactgggt agtggtcgcg ggaacggcaa aagtcaccat tgacggtgat
    14521 atcaaactgc ttggtgaaaa cgagtccatt tatattccgc tgggggcgac gcattgcctg
    14581 gaaaacccgg ggaaaattcc gctcgattta attgaagtgc gctccggctc ttatctcgaa
    14641 gaggatgatg tggtgcgctt cgcggatcgc tacggacggg tgtaaacgtc gcatcaggcg
    14701 ctgtttgtcg gatgcggcgt gaacgcctta tccgacctac ggttcggatt tgtaggcctg
    14761 ataagacgcg gcagcgtcgc atcaggcaat gaatgcgaaa ccgagatgta aataacgaca
    14821 aaaacaaaat tggccgtttc ggtcagggcc aactattgcc tgaaaaaggg taacgacatg
    14881 aaaaaattaa cctgctttaa agcctatgat attcgcggta aattaggcga agaactgaat
    14941 gaagatattg cctggcgcat tgggcgtgcc tatggcgaat ttctcaaacc gaaaaccatt
    15001 gtgttaggcg gtgatgtccg tctcaccagc gaaaccttaa aactggcgct ggcgaaaggt
    15061 ttacaggatg cgggcgtcga tgtgctggat attggtatgt ccggcaccga agagatctat
    15121 ttcgccacgt tccatctcgg cgtggatggc ggcattgaag ttaccgccag ccataatccg
    15181 atggattaca acggaatgaa gctggtgcgc gaaggggctc gcccgatcag cggtgatacc
    15241 ggactgcgcg acgtccagcg tctggctgaa gccaacgact ttcctcccgt cgatgaaacc
    15301 aaacgcggtc gctatcagca aatcaacctg cgtgacgctt acgttgatca cctgttcggt
    15361 tatatcaatg tcaaaaacct cacgccgctc aagctggtga ttaactccgg gaatggcgca
    15421 gcgggtccgg tggtggacgc cattgaagcc cgctttaaag ccctcggcgc gcccgtggaa
    15481 ttaatcaaag tacacaacac accggacggc aatttcccca acggtattcc taacccgctg
    15541 ctgcctgaat gccgcgacga cacccgcaat gcggtcatca aacacggcgc ggatatgggc
    15601 attgcctttg atggcgattt tgaccgctgt tttctgtttg acgaaaaagg gcagtttatc
    15661 gagggctact acattgtcgg cctgctggca gaagcgttcc tcgaaaaaaa tcccggcgcg
    15721 aagatcatcc acgatccgcg tctctcctgg aataccgttg atgtggtgac tgccgcaggc
    15781 ggcaccccgg taatgtcgaa aaccggacac gcctttatta aagaacgtat gcgcaaggaa
    15841 gacgccatct acggtggtga aatgagcgcc caccattact tccgtgattt cgcttactgc
    15901 gacagcggca tgatcccgtg gctgctggtc gccgaattgg tgtgcctgaa agagaaaacg
    15961 ctgggcgaac tggtgcgcga ccggatggcg gcgtttccgg caagcggtga gatcaacagc
    16021 aaactggcgc aacccgttga ggcgattaac cgcgtggaac agcattttag ccgtgaggcg
    16081 ctggcggtgg atcgcaccga tggcatcagc atgacctttg ccgactggcg ctttaacctg
    16141 cgcacctcca ataccgaacc ggtggtgcgc ctgaatgtgg aatcgcgcgg tgatgtgccg
    16201 ctgatggaag cgcgaacgcg aactctgctg acgttgctga acgagtaatg tcggatcttc
    16261 ccttacccca ctgcgggtaa ggggctaata acaggaacaa cgatgacaaa tctaaaaaag
    16321 cgcgagcgag cgaaaaccaa tgcatcgtta atctctatgg tgcaacgctt ttcagatatc
    16381 accatcatgt ttgccggact atggctggtt tgcgaagtca gcggactgtc attcctctac
    16441 atgcacctgt tggtggcgct gattacgctg gtggtgttcc agatgctggg cggcatcacc
    16501 gatttttatc gctcatggcg cggtgttcgg gcagcgacag aatttgccct gttgctacaa
    16561 aactggacct taagcgtgat tttcagcgcc ggactggtgg cgttcaacaa tgatttcgac
    16621 acgcaactga aaatctggct ggcgtggtat gggctgacca gcatcggact ggtggtttgc
    16681 cgttcgtgta ttcgcattgg ggcgggctgg ctgcgtaatc atggctataa caagcgcatg
    16741 gtcgcggtgg cgggggattt agccgccgga caaatgctga tggagagctt ccgtaatcag
    16801 ccgtggttag ggtttgaagt ggtgggcgtt taccacgacc cgaaaccggg cggcgtttct
    16861 aacgactggg cgggcaatct gcaacagctg gtcgaggacg cgaaagcggg caagattcat
    16921 aacgtctata tcgcgatgca aatgtgcgac ggcgcacgag tgaaaaaact ggtccatcaa
    16981 ctggcggaca ccacctgttc ggtgctgctg atccccgacg tctttacctt caacattctc
    17041 cattcacgtc tcgaagagat gaacggcgtt ccggtggtgc cgctgtacga tacgccgctt
    17101 tccggggtta accgcctgct caaacgtgcg gaagacattg tgctggcgac gcttatcctg
    17161 ctgctgatct ccccggtgct gtgctgcatt gcgctggcgg tgaaactcag ttcacctggg
    17221 ccggttattt tccgccagac tcgctacggc atggatggca agccgatcaa agtgtggaag
    17281 ttccgttcca tgaaagtgat ggagaacgac aaagtggtga ctcaggcgac gcagaacgat
    17341 ccgcgcgtca ctaaagtggg gaactttctg cgccgcacct cgctggatga actgccgcag
    17401 tttatcaatg tgctgaccgg cgggatgtcg attgtcggtc cgcgtccgca cgcggtagcg
    17461 cataacgaac agtatcgcca gctcattgaa ggctacatgc tgcgccacaa agtgaaaccg
    17521 ggcattaccg gctgggcgca gattaatggc tggcgcggcg aaaccgacac gctggagaaa
    17581 atggaaaaac gcgtcgagtt cgaccttgag tacatccgcg aatggagcgt ctggttcgat
    17641 atcaaaatcg ttttcctgac ggtgttcaaa ggtttcgtta acaaagcggc atattgatat
    17701 gagcttacgt gaaaaaacca tcagcggcgc gaagtggtcg gcgattgcca cggtgatcat
    17761 catcggcctc gggctggtgc agatgaccgt gctggcgcgg attatcgaca accaccagtt
    17821 cggcctgctg actgtgtcgc tggtgattat cgcgctggca gatacgcttt ctgacttcgg
    17881 tatcgctaac tcgattattc agcgaaaaga aatcagccac cttgaactca ccacgttgta
    17941 ctggctgaac gtcgggctgg ggatcgtggt gtgcgtggcg gtgtttttgt tgagtgatct
    18001 catcggcgac gtgctgaata acccggacct ggcaccgttg attaaaacat tgtcgctggc
    18061 gtttgtggtg atcccccacg ggcaacagtt ccgcgcgttg atgcagaagg agctggagtt
    18121 caacaaaatc ggcatgatcg aaaccagcgc ggtgctggcg ggcttcactt ttacggtggt
    18181 tagcgcccat ttctggccgc tggcgatgac cgcgatcctc ggttatctgg tcaatagtgc
    18241 ggtgagaacg ctgctgtttg gctactttgg tcgaaaaatt tatcgccccg gtctgcattt
    18301 ctcgctggcg tctgtggcac caaacttacg ttttggtgcc tggctgacgg cggacagcat
    18361 catcaactat ctcaatacca acctttcaac gctggtgctg gcgcgtattc tcggcgcggg
    18421 cgtggcaggg ggatacaacc tggcgtacaa cgtggccgtt gtgccgccga tgaagctgaa
    18481 cccaatcatc acccgcgtgt tgtttccggc gtttgccaaa attcaggacg ataccgaaaa
    18541 gctgcgcgtc aacttctaca agctgctgtc ggtggtgggg attatcaact ttccggcgct
    18601 gctcgggctg atggtggtgt caaataactt tgtaccgctg gtatttggcg aaaagtggaa
    18661 cagcattatt ccggtgctgc aattgctgtg tgtggtgggg ctactgcgct ccgtgggtaa
    18721 cccgattggt tcgctgctga tggcgaaagc gcgggtcgat atcagcttta aattcaacgt
    18781 attcaaaaca tttctgttta ttccggcgat tgttattggt gggcagatgg cgggcgcaat
    18841 cggcgtcacg ctcggtttcc tgctggtgca gattatcaac accattctga gttacttcgt
    18901 gatgattaaa ccggttcttg gttccagtta tcgccagtac atcctgagtt tgtggctgcc
    18961 gttttatctc tcgttgccga cgctggtggt cagttatgcg ctgggcattg tgctgaaagg
    19021 gcaactggcg ctggggatgc tgctggcggt gcaaatagcc gcgggcgtgc tggcgtttgt
    19081 ggtgatgatt gtgctgtcgc gccatccgct ggtggtggaa gtgaagcgtc agttttgtcg
    19141 cagcgaaaaa atgaaaatgc ttttacgggc ggggtgagtt gttgttttcg gttccggcaa
    19201 cgattgccgg atgcggcgta aaacgcctta tccatcctac ggttctgtgc tcgggtcttg
    19261 taggcctgat aagacgcgtc agcgtcgcat caggcaaaaa gcggcaacga acaaaacaaa
    19321 cgcagcctca cctggcatca aatttatgag gaaaaaatga aattacttat tctgggcaac
    19381 cacacttgcg gcaaccgcgg cgacagcgcc atcctgcgcg gcttacttga tgccatcaac
    19441 agcctcaatc cacacgccga agtggatgtg atgagccgct atccggtcag ttcttcctgg
    19501 ctgctcaacc gcccggtaat gggcgatccg ctgttcctgc aaatgaaaca acacaacagc
    19561 gcggcgggcg ttgtcgggcg cgttaaaaaa gtcctccgtc gccgctacca gcatcaggta
    19621 ttgctctcac gcgtcaccga cactggcaag ctgcgcaata tcgccatcgc ccagggattc
    19681 accgacttcg tgcgcctgct gtcaggttac gacgccatta tccaggtcgg cggatcgttt
    19741 tttgtcgatc tctatggcgt gccgcagttt gaacatgcac tttgcacttt catggcgaaa
    19801 aagccgctgt ttatgattgg tcacagcgtc ggtcccttcc aggatgagca atttaaccaa
    19861 ctggcgaatt acgtttttgg tcactgtgac gcgctgatcc tacgcgaatc ggtcagtctc
    19921 gaactgatga aacgcagcaa tatcaccact gcaaaagtgg aagatggcgt cgataccgcg
    19981 tggctggtcg atcaccacac agaagatttc accgccagct atgccgtcca acactggctg
    20041 gacgttgccg cacaacagaa aacggtggca attaccctgc gcgaactggc accgttcgac
    20101 aaacgtctcg gcaccactca acaagcgtat gaaaaagcct ttgccggggt ggtcaatcgc
    20161 attctcgacg aagggtatca ggtgattgcg ctatccacct gtacgggcat cgacagctat
    20221 aacaaagacg accgcatggt ggcgctcaac ctacgccagc acatcagcga tcctgcccgt
    20281 tatcacgtag tgatggatga actcaacgat ctggaaatgg gcaaaattct gggggcctgt
    20341 gaactcaccg tcggtacgcg cctgcactcc gccattatct caatgaactt tgctactccg
    20401 gcgattgcca tcaactatga acataaatcc gccgggatta tgcagcagtt gggactaccg
    20461 gagatggcga ttgatatccg tcatttactg gacggcagcc tgcaagcgat ggttgcggat
    20521 accttaggcc agcttccggc gctgaacgca cgacttagcg aagccgtcag tcgtgagcgt
    20581 cagacaggaa tgcagatggt gcagtccgta ctggaacgca ttggggaggc gaaatgaagg
    20641 ttggcttctt tttactgaaa tttccgctgt cgtcggaaac cttcgtcctc aaccaaatta
    20701 ccgcgtttat tgatatgggc tttgaggtgg agattgtcgc gctgcaaaaa ggcgacacac
    20761 aaaacaccca cgcggcatgg acgaaataca accttgctgc cagaacccgc tggttacagg
    20821 acgaacctac gggcaaagtg gcgaaactgc gccaccgagc cagccagacc ttacgcggca
    20881 ttcatcgtaa aaatacctgg caggcgctca acctcaaacg ctatggcgcc gagtcgcgga
    20941 acctgatttt gtctgccatt tgcggccagg tcgcaacacc attttatgcc gatgtcttta
    21001 tcgctcattt tggcccggcg ggggtaaccg cagcaaaact acgcgaactg ggtgtcattc
    21061 gcggcaaaat cgccaccatc ttccacggca ttgatatttc cagtcgggaa gtgctcaacc
    21121 attacactcc cgaatatcag caactgtttc gccgtggcga cctgatgtta ccgataagcg
    21181 atttgtgggc cggaaggctg caaaaaatgg gctgccccag agaaaaaatc gccgtatcgc
    21241 gtatgggcgt ggacatgacg cgctttagcc cgcgtccggt gaaagcgccc gcaacgccgc
    21301 tggaaatcat ttccgtcgca cgcttaaccg aaaaaaaagg cctgcatgtg gcgatcgaag
    21361 cctgccgtca gttgaaagag cagggcatgg catttcgcta tcgcatcctc ggcattggcc
    21421 cgtgggaacg acgcctgcgt accctcatcg aacaatatca actggaagat gtggtggaga
    21481 tgccgggctt taaaccgagc catgaagtga aagcgatgct cgacgacgcg gatgtcttcc
    21541 tgttgccatc ggttacaggt gcggatggtg atatggaagg tattccggtg gcgctaatgg
    21601 aagcaatggc ggtcggcatt ccggttgttt caactctgca tagcggaata ccagaactgg
    21661 ttgaggccga taaatccggc tggctggtgc ctgagaacga tgctcgcgca ctggcgcaac
    21721 gactggcggc gtttagccaa ctggacaccg acgaactggc tccggttgtc aaacgtgcgc
    21781 gcgaaaaagt cgaacacgat tttaaccagc aggtgattaa tcgagaactc gccagcttgt
    21841 tacaggcttt atagaggaga acgtatgcca tttaaaaaac tctcccgacg caccttcctg
    21901 acggcaagct cggcgcttgc cttcctccat acccctttcg cccgtgcact tcccgcccga
    21961 caaagcgtta acattaacga ctacaaccca cacgactgga tcgcctcatt taaacaagcc
    22021 ttcagcgaag ggcaaacagt cgtcgtgtct gccggattgg tttgtgacaa tatcaacacc
    22081 ggcatcttca ttcctgcagg taaaacgtta cacatccttg gaagcctgcg cggcaacggc
    22141 agagggcgat ttgtcttaca ggacggcagc caggtgacag gggaggaggg cggcagtatg
    22201 cataacatca ccctggatgt gcgtggttct gactgcacca tcaaagggct ggcgatgagc
    22261 ggctttggcc cagtaacgca gatttacatc ggcggcaaaa acaaacgggt catgcgcaac
    22321 ctgatcatcg ataacctgac cgttagccac gctaactacg ccatcttacg ccagggattt
    22381 cataaccaga ttatcggtgc caacatcacc aattgtaagt tcagcgactt acaaggcgac
    22441 gccattgaat ggaacgtggc aattaacgac cgcgatattt tgatctccga ccatctcatc
    22501 gagcgcatca actgtaccaa cggcaaaatc aactggggca tcggcatagg ccttgcggga
    22561 agtacctacg ataataatta tccggaaaac caggcagtga aaaactttgt cgtggcgaat
    22621 atcacgggat cggattgtcg acagttgatc catgttgaaa atggtaaaca ttttgttatt
    22681 cgtaatatca aagcccgtaa tatcacaccg gatttcagta agaaagcagg tattgataac
    22741 gccacggtcg ctatttacgg ttgtgataat ttcgtgattg ataatattga aatgattaat
    22801 agtgccggga tgttaatcgg ctatggggta attaaaggca aatatctctc gataccgcag
    22861 aatttccggg tgaataatat tcaactggat aacacccacc ttgcttataa attgcgcggc
    22921 atccaaatct ccgccgggaa tgctgtctcc tttgtggcgc tgactaacat tgagatgaag
    22981 cgtgcgtcat tggagttaca caacaagccg caacatcttt ttatgcgtaa tatcaaggtg
    23041 atgcaggaat cctcagttgg accagcattg agcatgaact ttgacatgcg caaagacgtt
    23101 cgaggcgtgt ttatggcgaa aaaagaaaca ctgctgtctc ttgcaaatgt tcatgcggtg
    23161 aatgaaaaag ggcaaagctc cgtcgagatc gacaggatta atcaccatat tgttaatgtg
    23221 gaaaagatta actttagatt gccggaacgg agggagtaga tttgcgacca ttcataaact
    23281 tactggatat aatgttctaa aagattataa tcctcacact aactgttaaa gctggggctg
    23341 ggcagcattg actactcagg gaaggtactc atctttatct tgtgtttctt acgtaacaac
    23401 cagactaaag tgatttatga acgataacgt tttgctcata ggagcttccg gattcgtagg
    23461 aacccgacta cttgaaacgg caattgctga ctttaatatc aagaacctgg acaaacagca
    23521 gagccacttt tatccagaaa tcacacagat tggcgatgtt cgtgatcaac aggcactcga
    23581 ccaggcgtta gccggttttg acactgttgt gctactggca gcggaacacc gcgatgacgt
    23641 cagccctact tctctctatt atgatgtcaa cgttcagggt acccgcaatg tgctggcggc
    23701 catggaaaaa aatggcgtta aaaatatcat ctttaccagt tccgttgctg tttatggttt
    23761 gaacaaacac aaccctgacg aaaaccatcc acacgatcct ttcaaccact acggcaaaag
    23821 taagtggcag gcagaggaag tgctgcgtga atggtataac aaagcaccaa cagaacgttc
    23881 attaaccatc atccgtccta ccgttatctt cggtgaacgg aaccgcggta acgtctataa
    23941 cttgctgaaa cagatcgctg gcggcaagtt tatgatggtg ggcgcaggga ctaactataa
    24001 gtccatggct tatgttggaa acattgttga gtttatcaag tacaaactga agaatgttgc
    24061 cgcaggttat gaggtttata actacgttga taagccagac ctgaacatga accagttggt
    24121 tgctgaagtt gaacaaagcc tgaacaaaaa gatcccttct atgcacttgc cttacccact
    24181 aggaatgctg ggtggatatt gctttgatat cctgagcaaa attacgggca aaaaatacgc
    24241 tgtcagctca gtgcgcgtga aaaaattctg cgcaacaaca cagtttgacg caacgaaagt
    24301 gcattcttca ggttttgtgg caccgtatac gctgtcgcaa ggtctggatc gaactctgca
    24361 gtatgaattc gttcatgcca aaaaagacga cataacgttt gtttctgagt aatgcagacg
    24421 agtcagagaa aaaaggacca ggttggacta cgattattcg gaaagaggta ctgattaaat
    24481 tatcagtatg cggaattccg agtaatccga gtaacccaga tgctgatggt gtgtagtgtt
    24541 attctaaggt ttaagcaaag ttatgcggaa ctaagctttg gtatatagcc ataacgtttg
    24601 gctaacctat ggcaataatt tacgcactat cagccactta gaggtaggaa aagaatgacg
    24661 aatttaaaag cagttatacc ggtagcaggt cttgggatgc atatgttgcc tgccactaag
    24721 gcgattccca aagagatgct accgatcgtc gacaagccaa tgattcagta catcgttgac
    24781 gagattgtgg ctgcagggat caaagaaatc ctcctggtaa ctcacgcgtc caagaacgcg
    24841 gtcgaaaacc acttcgacac ctcttatgaa ttagaatctc tccttgaaca gcgcgtgaag
    24901 cgtcaactac tggcggaagt acagtccatt tgcccgccgg gcgtgaccat tatgaacgta
    24961 cgtcagggcg aacctttagg tttgggccac tccattttat gtgcacgacc agctattggt
    25021 gacaacccat ttgtcgtggt actgccggac gttgttatcg acgacgccag cgctgacccg
    25081 ctgcgctaca accttgctgc catgattgcg cgcttcaacg aaacgggccg cagccaggtg
    25141 ctggcaaaac gtatgccggg cgatctctct gaatactccg tcattcagac caaagaaccg
    25201 ctggatcgtg aaggtaaagt cagccgcatt gttgaattta tcgaaaaacc ggatcaaccg
    25261 cagacgctgg actcagacat tatggccgtt ggtcgctatg tgctttctgc cgatatttgg
    25321 ccggaactgg aacgcactca gccaggtgca tgggggcgta ttcagctgac tgatgccatt
    25381 gctgaactgg cgaaaaaaca gtcagttgat gcaatgttaa tgacggggga cagctacgat
    25441 tgcggtaaaa aaatgggcta catgcaggca tttgtgcagt acggactacg caacctgaaa
    25501 gaaggggcaa agttccgtaa agggattgag aagctgttaa gcgaataaaa tggttaagaa
    25561 ggaaattata acggcaatga agatttgttc gaaagttgtg tattgaaaat attcttgccg
    25621 ctgatttttt atagctaatc atattagcaa gaggtttata agaagatgtt agtcaagttt
    25681 ttccgggatt taccttgttt tcagagtcga tagggaagac aattagcgtt tagatttttc
    25741 tggattggtt agagagtgta atgctcgtta cattgtagat atgtatacag tgctctggta
    25801 gctgttaagc caagggcggt agctatttga aatcatacta aaacaatatg attattgcaa
    25861 aaggtgataa aatgatcggt attattctgg ctgcaggtgt tgggtctcgt ttgcggccca
    25921 tgactaatag taaacctaag tgtcttgtga ctgttgctgg caagcctatc ttggattatc
    25981 agttaaactc ctatcgcctg gctggtat
//
LOCUS       DBANUU010000060        26007 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000060 DBANUU010000000
VERSION     DBANUU010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.060"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(66..146)
                     /gene="shoB"
                     /locus_tag="ACIE68_002452"
     CDS             complement(66..146)
                     /gene="shoB"
                     /locus_tag="ACIE68_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFX5908759.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(567..947)
                     /gene="acpS"
                     /locus_tag="ACIE68_002453"
     CDS             complement(567..947)
                     /gene="acpS"
                     /locus_tag="ACIE68_002453"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFX5908760.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGDRLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(947..1678)
                     /gene="pdxJ"
                     /locus_tag="ACIE68_002454"
     CDS             complement(947..1678)
                     /gene="pdxJ"
                     /locus_tag="ACIE68_002454"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFX5908761.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1690..2418)
                     /gene="recO"
                     /locus_tag="ACIE68_002455"
     CDS             complement(1690..2418)
                     /gene="recO"
                     /locus_tag="ACIE68_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFX5908762.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2430..3335)
                     /gene="era"
                     /locus_tag="ACIE68_002456"
     CDS             complement(2430..3335)
                     /gene="era"
                     /locus_tag="ACIE68_002456"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFX5908763.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3332..4012)
                     /gene="rnc"
                     /locus_tag="ACIE68_002457"
     CDS             complement(3332..4012)
                     /gene="rnc"
                     /locus_tag="ACIE68_002457"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFX5908764.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4284..5258)
                     /gene="lepB"
                     /locus_tag="ACIE68_002458"
     CDS             complement(4284..5258)
                     /gene="lepB"
                     /locus_tag="ACIE68_002458"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFX5908765.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGLRLSRIGGIH"
     gene            complement(5274..7073)
                     /gene="lepA"
                     /locus_tag="ACIE68_002459"
     CDS             complement(5274..7073)
                     /gene="lepA"
                     /locus_tag="ACIE68_002459"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFX5908766.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7271..7750)
                     /gene="rseC"
                     /locus_tag="ACIE68_002460"
     CDS             complement(7271..7750)
                     /gene="rseC"
                     /locus_tag="ACIE68_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFX5908767.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7747..8703)
                     /gene="rseB"
                     /locus_tag="ACIE68_002461"
     CDS             complement(7747..8703)
                     /gene="rseB"
                     /locus_tag="ACIE68_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFX5908768.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8703..9353)
                     /gene="rseA"
                     /locus_tag="ACIE68_002462"
     CDS             complement(8703..9353)
                     /gene="rseA"
                     /locus_tag="ACIE68_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFX5908769.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEMLHFDISSRVMAAIEEEPVRQPAALIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9386..9961)
                     /gene="rpoE"
                     /locus_tag="ACIE68_002463"
     CDS             complement(9386..9961)
                     /gene="rpoE"
                     /locus_tag="ACIE68_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFX5908770.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9958..10113)
                     /gene="rseD"
                     /locus_tag="ACIE68_002464"
     CDS             complement(9958..10113)
                     /gene="rseD"
                     /locus_tag="ACIE68_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFX5908771.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10369..11991
                     /gene="nadB"
                     /locus_tag="ACIE68_002465"
     CDS             10369..11991
                     /gene="nadB"
                     /locus_tag="ACIE68_002465"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFX5908772.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(11976..12713)
                     /gene="trmN"
                     /locus_tag="ACIE68_002466"
     CDS             complement(11976..12713)
                     /gene="trmN"
                     /locus_tag="ACIE68_002466"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFX5908773.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWVTQQTARFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12845..14179
                     /gene="srmB"
                     /locus_tag="ACIE68_002467"
     CDS             12845..14179
                     /gene="srmB"
                     /locus_tag="ACIE68_002467"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFX5908774.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(14388..15269)
                     /gene="yfiE"
                     /locus_tag="ACIE68_002468"
     CDS             complement(14388..15269)
                     /gene="yfiE"
                     /locus_tag="ACIE68_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFX5908775.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            15372..15959
                     /gene="eamB"
                     /locus_tag="ACIE68_002469"
     CDS             15372..15959
                     /gene="eamB"
                     /locus_tag="ACIE68_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFX5908776.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQQLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(16015..16398)
                     /gene="grcA"
                     /locus_tag="ACIE68_002470"
     CDS             complement(16015..16398)
                     /gene="grcA"
                     /locus_tag="ACIE68_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFX5908777.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            16703..17392
                     /gene="ung"
                     /locus_tag="ACIE68_002471"
     CDS             16703..17392
                     /gene="ung"
                     /locus_tag="ACIE68_002471"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFX5908778.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(17440..18477)
                     /gene="yfiF"
                     /locus_tag="ACIE68_002472"
     CDS             complement(17440..18477)
                     /gene="yfiF"
                     /locus_tag="ACIE68_002472"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFX5908779.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            18684..19103
                     /gene="trxC"
                     /locus_tag="ACIE68_002473"
     CDS             18684..19103
                     /gene="trxC"
                     /locus_tag="ACIE68_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFX5908780.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            19172..19870
                     /gene="tapT"
                     /locus_tag="ACIE68_002474"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             19172..19870
                     /gene="tapT"
                     /locus_tag="ACIE68_002474"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFX5908781.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            19902..22562
                     /gene="pat"
                     /locus_tag="ACIE68_002475"
     CDS             19902..22562
                     /gene="pat"
                     /locus_tag="ACIE68_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFX5908782.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            22676..24031
                     /gene="pssA"
                     /locus_tag="ACIE68_002476"
     CDS             22676..24031
                     /gene="pssA"
                     /locus_tag="ACIE68_002476"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFX5908783.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            24077..24400
                     /gene="yfiM"
                     /locus_tag="ACIE68_002477"
     CDS             24077..24400
                     /gene="yfiM"
                     /locus_tag="ACIE68_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFX5908784.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(24397..25695)
                     /gene="kgtP"
                     /locus_tag="ACIE68_002478"
     CDS             complement(24397..25695)
                     /gene="kgtP"
                     /locus_tag="ACIE68_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFX5908785.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(25812..>26007)
                     /locus_tag="ACIE68_002479"
     CDS             complement(25812..>26007)
                     /locus_tag="ACIE68_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908786.1"
                     /translation="KAIRQDGLFALAQKKMPDATLRVLYSHICQIQQRMRFPQLAYFH
                     TCPPYQKFILKLLNNHFPAN"
BASE COUNT         6282 a         6869 c         6487 g         6369 t
ORIGIN      
        1 atttatagtt attaaaggcg cgtccgattg gttcaccgga cgcaccttaa gtacgtttcc
       61 ttgtgttata agaacagaag gatcagctgt aaaacagcaa tgatgatttt gatgacccgt
      121 ttaatcaggt atcggcaatc agtcattcgt ttttccttaa acaaggaatg cagccgttca
      181 gattagccct tacatctccc caaaactgaa cgtgcgagtt attgagggtg catgctgcac
      241 tccacaccag agctttgacg acaccactcg tttcaatggg ggaattctgt ggcatggtgt
      301 aaagcacagc aaaatcttca ataacgaagc caattttaat gtacttacga attggcggtc
      361 acgttaatct ttccatcaat attgctttct tcgtaaaggc tcgagttttt atgctaaaga
      421 ttgcaagttg cttgtaaaag ataagtacac tgatccataa tcgctgttgt tgagggtgca
      481 tgctgcacaa aattaaagtt aaaaagtaaa acccccgttc cttaccagtt cgggggtttt
      541 actttttaaa gagaacggta ttatttttaa ctttcaataa ttaccgtggc acaagcatag
      601 tgccgctcat ctgccagcgt tacatgcata tttgcaacgc ccagcttttc cgccagtttt
      661 aatgcctcgc cccatagccg tagccgtggt ttgccgagct catcattgaa tacttcaaat
      721 tgattaaacg ccagaccatt gcggatcccg gtgccaaacg cttttgctgc ggcttctttc
      781 acagcaaaac gcttcgccag aaaacgcacc ggctggtggt gcgttttcca gatagcccat
      841 tcgttatcgc ttaatacgcg gcgtgccagg cgatcaccgg atcgggcgat caccgcttcg
      901 atgcgagcga tctccacaat atccgtgcct aaacctaata ttgccattag ccacgcgctt
      961 ccagcatcag acgcttcatt tctgccaccg catctttcag tccggtcatc actgcacgac
     1021 caataatggc atgaccgata ttcagttcat gcatctcagg gatggcggca atggctttca
     1081 cgttgtgata ggtcagaccg tgtccggcgt taactttcag accgaggctt gcggcaaagg
     1141 tcgcggcttt ggcgatacgc gccagctctt gcgcctgttc ggcgtcagtt ttggcatcag
     1201 catagcaacc ggtgtggatc tcgataaacg gtgcgccaac ctctgccgca gctttgatct
     1261 gctcttcatc ggcgtcaata aacagagaaa cctgaatccc ggcatctgcc agacgtttgc
     1321 aggcatcgcg cattttgtca cgctgccctg cgacatccag gccgccttcg gttgttactt
     1381 cctgacgctt ttccggtacc aggcagcaaa aatgtggctt cgtctcaacg gcgatcgcca
     1441 gcatctcttc ggtcaccgcc atctccagat tcatgcgggt atccagcgtc tgacgcagga
     1501 tgcgcacgtc gcggtcagta atgtgacggc gatcttcacg taaatgcacg gtaatgccgt
     1561 ccgctcccgc ctgctcggca ataaacgcgg cctgcaccgg atccgggtaa gcggtaccgc
     1621 gcgcgttgcg cagcgtagcg atatggtcaa tgttgacgcc taacagtaat tcagccatga
     1681 caatcctcat cattcataat gtgttttcac cgttcgctta ggcataaatt gccggaacag
     1741 ttccctgctc tttaaaggtt taccgccaag atacggctta agcgccatgc gggtaaagcg
     1801 tttcgcagcg cgcagtgtgt ctgcgtcagg aaattcccgt gcgtttaacg cttttaactg
     1861 ccttccggtg aacgttttat tgtcgataac gacgcttgcg ataaaccctt tttcttcgcg
     1921 ataacgatac gtcatggtgt catctaccgg ctcgccgcta cccgcacaat gggtaaaatt
     1981 gacgccataa cccagatgcc cgagcagtgc cagttcaaag cggcgcagcg cgggttctgg
     2041 cgtaccagtg acccctgcaa gagactgaat gcagtgcaag taatcgaaaa aaagttcaga
     2101 gaagcgcgtc tcgtattcca gtacgcggga gagaagttcg ttgatgtaca gaccgctgta
     2161 aagcgtgata ccgcttaatg gcagcgccag cgagacggct tcagcactgc gcagcgtttt
     2221 gacttcgcca cgcccgccaa aacgtagcaa gagaggggtg aaaggctgta atgcaccttt
     2281 cagggtagag cgtttagagc gtgcgccttt ggcaaccaga cgcacgcgcc ccgattcctc
     2341 cgtgaagacg tccagcatca ggctggtttc gctccacggg cgactatgca ggacaaatgc
     2401 gcgctgccag ccttccatcg gagttactct taaagatcgt caacgtaacc gagactgcgc
     2461 agtgcgcgtt cgtcgtcggc ccaaccggat ttcactttta cccacagttc aaggtgaaca
     2521 ggcgcttcga acatttcctg catgtcttta cgcgcttcaa tcccgatggt tttgatcttg
     2581 gcccctttgt tgccaatgac catcttcttc tgcccttcac gctcaacgag aatcaaaccg
     2641 ttgatgtcat aaccaccgcg ttcgttagag acgaaacgtt cgatctccac ggtcacggag
     2701 tacggcagtt cagcgccgag gaaacgcatc agtttttcgc ggatgatttc agacgccata
     2761 aaacgctgtg agcgatcggt gatgtaatct tccgggaagt gatgagtcgc ttcaggtaga
     2821 tgcttacgca cgattgccgc aatagtgtca acattcagcc cggtttcggc agagattggc
     2881 acgatatcga ggaagttcat ctggcttgcc aggaactgca ggtgcggcag cagatcggct
     2941 ttctcctgca cgttgtccac tttgttcacc gcgaggatta ccggcgcttt gccttcgcgc
     3001 agtttgttga gcaccatttc gtcgtccggc gtccagcggg tgccttcaac gacaaaaatc
     3061 accagctcaa catcgccaat agagctgctc gccgctttgt tcatcaggcg gttaatggcg
     3121 cgtttttctt ccatatgcag gcccggtgta tcgacgtaga tcgcctgata cgcgccttca
     3181 gtatggatcc ccacaatgcg gtgacgagtt gtctgcgcct tgcgggaagt gatggagatt
     3241 ttctgcccca gcagtttgtt caacaatgtg gatttgccaa cgttcggacg tccgacgatg
     3301 gcaataaatc cgcagtaact tttatcgatg ctcattccag ctccagtttt ttcaacgcct
     3361 gttcggcggc agcctgctca gccttacgac ggcttgaacc tgtgccaacc accggttcac
     3421 tcaggccgct gacctggcag tggatagtaa attcctgatc gtgcgcttcg ccacgtacct
     3481 ggactaccag ataagtcggc agcggcagat ggcgaccctg caaatattct tgcaagcgcg
     3541 ttttcggatc tttttgttta tcgcctgggc taatttcgtc caaacgagtt tgataccagt
     3601 tgaggattaa tttctcgacg gtttgaatat cactgtcgag gaatacgcca ccaattaatg
     3661 cttcgacggt gtcggcgaga attgactcac gacgaaatcc accgctttta agttcacctg
     3721 gccctaaacg taagcactcg cctaactcaa attcgcgcgc cagttccgcc agcgtattgc
     3781 cacggaccag cgtggcgcgc atccggctca tatcgccttc atccacacga gggaaacggt
     3841 gataaagcgc attggcgata acgtagctca gaatagagtc gcctaaaaat tctaaacgct
     3901 cgttatgttt actgctggca ctacgatgag ttaatgcctg ctgcaacagt tcctgatgat
     3961 taaaagtgta gcccagcttc cgttgaagcc gattaattac gatggggttc atgcgatacc
     4021 aataaataaa tgcgtcaaca attcagcaca cgaaacagac ctgatataca tggttctgct
     4081 aactgcttcg ctgcagtttc agtttaccgg tatatgggga ccaacgctgt ttcgtgtgcc
     4141 gtggcaacct ggaggtgcca accttaaact tcgggggaat attctataca caacgacggg
     4201 ggatgtcgtt agccacggga gatttatctc ataaataatt cacgttgtcg ccataacggc
     4261 gacaacgtga acgaagatgg ctattaatgg atgccgccaa tgcgacttaa gcgcagacca
     4321 gtcggccatt cgccttcttg cttatcgaag ctcatccaga tagccgttgc ccgaccgacc
     4381 agattcgctt ccggcacaaa gccccagtaa cggctgtccg cgctgttgtc gcggttgtcg
     4441 cccatcatga agtattgtcc cggaggaaca atccaggttg ccagttgttg ccctggctgc
     4501 tggtaataca tccccacctg atcctgcgca atcggcactg tcagaatgcg gtgcgtcaca
     4561 tcacccagtg tctctttacg ctcggaaaga cgaattccat tttctttggt ttcgtttttc
     4621 ggcacttcaa agaatccgct ggtcgcttcc ccaccattac ggcgtgagaa ggtctgaacg
     4681 aaatcgctcg gttccacgtt tgagtaggtg accggcagcg cgttttcaca cgcctggccg
     4741 gaactgcatc ccggttgaat cgtcagctct tttgagaccg gatcgtaagt gactttatcg
     4801 cccggtaaac ccaccgcgcg cttgatgtaa tcaagctttg gatcttccgg atatttaaag
     4861 accacgatat cgccgcgttt cggatgaccg gtttcgatca gcgttttctg gtagatagga
     4921 tctttaatgc cataagcaaa cttctctacc agaataaaat caccaattaa cagagtcggc
     4981 atcatcgaac ctgacgggat ctggaacggt tcataaataa acgaacgcac aatcaatacg
     5041 atagccagta ccggaaaaac agaagcaccg gtttccagcc agccaggctt cggcgcaacc
     5101 tttttcaacg ttgctttatc cagtgagtca ccggcagccg cctgcgccgc tgcctgacgt
     5161 tcccgccgtt taggtgcgaa aaagaattta tccacgcacc ataaaatgcc cgtcaccagt
     5221 gtggcaatca ccagaatcag ggcaaacata ttcgccatgc caactcctaa gggttatttg
     5281 ttgtctttgc cgacgtgcag aatggcgagg aacgcttcct gcggcagctc gacgttaccg
     5341 atctgtttca tgcgtttctt accttctttc tgcttctgca gcagcttttt cttacggctg
     5401 atatcgccgc cataacattt agccagtacg tttttacgca gctgtttcac ggtggatcgc
     5461 gcaataatgt gcgtaccaat cgctgcctga atggcgatat caaactgctg gcgtgggatc
     5521 agatctttca tcttctccac caactcgcga ccgcggtttt gcgaattatc acggtgggtg
     5581 atcagcgcca acgcatcaac acgttcaccg ttgattaata cgtctacacg taccatgtcg
     5641 gacgcctgga agcgtttgaa gttgtaatcc agagacgcat aaccacgcga ggtagatttc
     5701 aggcgatcga agaagtcgag caccacttcc gccatcggga tctcgtacgt cagcgccacc
     5761 tgattaccgt ggtaaaccat attggtctgc acgccgcgtt tttctacgca cagcgtaata
     5821 acgttgccga gatatgcctg cggcagcagc atgtgacatt cggcaatcgg ctcgcgcagt
     5881 tcgtagatgt tatttaccgc tggcagcttg gatggactgt cgacgtaaat aacttctctt
     5941 gacgtggttt caacttcata cactacggtc ggcgcagtgg tgatcagatc cagatcgtat
     6001 tcacgttcca gacgttcctg gatgatctcc atgtgcagca ggccgaggaa gccgcagcgg
     6061 aaaccaaagc ccagcgcgct ggagctttcc ggctcataga acagtgaagc atcgttcagg
     6121 ctgagtttac ccagcgcgtc acggaaggct tcatagtcgt cggaacttac cgggaacaga
     6181 ccggcgtata cctgcggttt gactttctta aagccaggca gcgccttttc tgccggatta
     6241 cgcgccagcg ttaaggtatc gccgactgga gcgccgtgga tatctttaat cgcacatacc
     6301 agccagccta cttcgccaca tttcagttca gtgcggtcaa cctgcttcgg cgtgaagatg
     6361 cccagacggt cggcgttata ggtctgcccg gtactcatga ctttcacttt gtcgccctta
     6421 cgcagggtgc cgtttttaat acggataagt gaaacaacgc ccagatagtt gtcgaaccag
     6481 gagtcgataa ttagtgcctg caacgggcct tccggatcgc cttccggcgg cggaatgtcg
     6541 cgcaccagac gttcgagaac gtcctgcacg ccaacgccgg ttttcgctga acagcgcacc
     6601 gcgtcggtgg cgtcgatgcc gacgatatct tcaatttctt ccgccacgcg ttcaggatcg
     6661 gctgccggca ggtcaatctt gttcagtacc ggcaccactt cgagatccat ttccatggcg
     6721 gtgtagcagt ttgccagggt ttgcgcttct acgccctgcc cggcgtcgac caccagcaat
     6781 gcaccttcac aggcagccag cgaacgggaa acttcatagg agaagtctac gtggcccggg
     6841 gtgtcgataa agttaagctg ataggtttcg ccgtcagacg ctttgtagtc cagcgtcacg
     6901 ctttgcgctt tgatggtaat gccacgctca cgctcaagat ccatggaatc gagaacctgc
     6961 gcctccattt cacggtcaga caggccaccg cagatctgga taatacggtc agacagcgtc
     7021 gatttaccgt ggtcaatgtg agctatgatc gaaaagttac gtatattctt cataaagtat
     7081 gattattgtg ccttaatgcc cggttaacca ggcttttaga agtcgctgtt ctgagcttaa
     7141 cgtctgtatt aatagaaacg ccgcattcta cactacaaca ttgaggcgag gaaatgttca
     7201 taccgtatgg attgtggtat ctggaaacgt cctcgcattt gttatgcaaa atgcaataaa
     7261 gccagtgaaa tcactggctc gcgtcttccg aagatgtttc aaatcgcaca aggccaggcg
     7321 gcaatgccac gcttaagatg atcggttgcc attctgcccg ggcagcaaac ttacgcgagt
     7381 agccgcgcgc aatcaggaac ccgccaatac caccgaggat cgcaccacat aatgctgcga
     7441 cgtcagaagc aaagagtagc tgaaatagcg aagcgatgag gaataatccc accagcggcg
     7501 acatataaac cagtaatgcg gagctaagca ggctgccttc ggcgatcccc aattccactt
     7561 tttgccccgg cactaacggc tcatcacagg gtacgacaat ggtatgcgtg gtttgcgggc
     7621 caagtttatt taacacgcgg ctaccgcaac cggcgcgtga agcgcaactg ctgcatgaag
     7681 ctttaacatc acaactgacc agcgcctgcc cgttttgcca ggagacgacg gtagcccact
     7741 ctttgatcat tgcgctgccc cgaacttaat attctcggca atgcgtttcg ccgtttgcgg
     7801 cggcagttca ccgacaatgg tgatttcggc gttatcacgt acgcttgtac tgacggttct
     7861 gcgtccggtg cgcaacatct gatcggtgct cgatggcgta gcgcggttaa cgtttaccga
     7921 gaagctgaat aatccgtcgg aatagagacg tgattcgata ggcatgttgt ccatcgtcgg
     7981 tagcggacgt cgactactgg aaacttcgct aaaaccctgt ggcaaccagg ttggcgtcca
     8041 gctgaattta gctttttcac ctacaggaac agaaagcaac ggcggcaaat ttgccttcgc
     8101 cagcgtctgc atactgctgc tgatatcctg attgacgtta aaagcaatca cgcgaaattg
     8161 ttccagcgtt tcaccatcgc gatcaaggag atcaacccgc atcggtaatt tcgattcggt
     8221 gtccatccac acgatgtagc tgtagcgtgt accatctcgg gcaaccacgc gaatgacttc
     8281 gcaaagacga tcagcaatac gcgtgcgccc gacggagata aagtcgtagt aaggagaaag
     8341 gcgtttgaaa tcggtataga taagcgatgg cagagaatca acaatgtaat cgccattaag
     8401 cgtgaacggt tcaagtcccg gttcaaaata gctgatttca ttgccgcgct gtaccacttc
     8461 ccggcgcggg ccatccattt gcaacaattg tgcaagaggg cggttatcga ggcgtgcatg
     8521 tcgataacgc agagactcaa caccctgttt attgatgctg atgaatgaca gctcgtaatt
     8581 cagtgactga ctggccaggt tcatctgctg taataacgcc ccggacgcgg gagtggccga
     8641 ggcgttagca gagaataaca ggctacctgt cactaatgac atggcaaacc aaagttgctt
     8701 cattactgcg attgcgttcc taaagtttga attcctggca cctgtacagc ggcttgctgg
     8761 gtttgcgcct gctcaaactg aagctgttca gagtggagtc ggcgttgcag ttcgtaatcc
     8821 tgcaacattg cattaatgcg acgacgctgc tcctgtacct gctgctgttg accattgttt
     8881 gcggtcgctt cagaaggtac tcccaggctt accgggctgg ctttacccat catcggcagt
     8941 gtattaaata ccggcgtttc gggctgctgg gacgtttcag attgtccatt atagtgctgg
     9001 acgccaacga taactgcaag cgatacgcat gctgctacgc ccatttgggt aagctgtgcc
     9061 gcccacggac gtactttctg ccagaatggc attttctgcc attgatgcgg cgcaggctgg
     9121 gcttccggga tcaatgccgc cggttgacgt actggctctt cttcaatggc ggccatcacg
     9181 cgtgaagaga tatcgaaatg gagcatctcg ggagtatcac cccgcattga gtcacggatt
     9241 aagtgatagc tttcccaggt tttctgcatt tctgggttat gagccagttc gttaagcagc
     9301 tcactatcca gcgtttcgcc atccattaaa gcggaaagtt gttctttctg catgcctaat
     9361 acccttatcc agtatcccgc tatcgtcaac gcctgataag cggttgaact ttgttatcaa
     9421 tagcttccct cgctcggaag atacgtgaac gcaccgtacc taccggacaa tccatgatag
     9481 cggctatctc ttcatagctc aggccatcca gctcccgcaa ggttattgcc atgcgcaaat
     9541 cttccgggag ggactcaata gttcggaaaa ctatctgtct cagttcttct gacaacatta
     9601 agttctcagg gttcgaaatt tctttcaacg cgccgccact ttcgaagttt tcagcttcaa
     9661 tggcatccac atcactggaa ggtggacgac gcccctgagc aaccaggtaa tttttcgctg
     9721 tatttacagc aatccgatac agccatgtat aaaaagcgct atctccccgg aacgaatcca
     9781 gcgcacgata ggctttaata aaagcttctt gtaccacatc gggaacatca cccgacggca
     9841 catagcggga aaccagactc gccactttat gctgatagcg cactaccagt aggttaaagg
     9901 ctttctgatc tcccttctgg acccgttcaa ccaggacctg gtccgttaac tgctcgctca
     9961 tccgaggtaa agtctcccca aaccaaattt ccacgcgcta tcgaaacgcc actccattag
    10021 ctgcaatttg agcaagcaaa gggttagagt gtctcgtttt tgtaaagttc cgtaacgcat
    10081 ctgtttttgt ttgtcatgct gtagacggat cattatctat cattataagt ctacagaatc
    10141 tgaacatcgc attatctgtg tagaaatgcc catttaactg cctgaagagt aacccaacgg
    10201 cctttttatt tcaccaccta atcctccacc agccagtaac ttctcttttt ctcgccgccc
    10261 tgcgtcagcg tgtttagcca ctgtaacaaa tattaaaata gcaggtgttt attcgcacaa
    10321 catgatgcta tgctgaccaa acggtgttta gtaaattaaa caaagaaaat gaatactctc
    10381 ccagaacatt catgtgacgt gttgattatc ggtagcggcg cagccggact ttcactggcg
    10441 ctacgcctgg ctgaccagca tcaggtcatc gttctaagta aaggcccggt aacggaaggt
    10501 tcaacatttt atgcccaggg cggtattgcc gccgtgtttg atgaaactga cagcattgac
    10561 tcgcatgtgg aagacacatt gattgccggg gctggtattt gcgatcgcca tgcagttgaa
    10621 tttgtcgcca gcaatgcacg atcctgtgtg caatggttaa ttgaccaggg ggttttgttt
    10681 gatacccacg ttcaaccgaa tggcgaagaa agctaccatc tgacccgtga aggtggacat
    10741 agtcaccgtc gtattcttca tgccgccgac gccaccggta gagaagtaca atccacgctg
    10801 gtgagcaagg cgcagaacca tccgaatatt cgtgtgctgg agcgcagcaa cgcggttgat
    10861 ctgattgtct ctgacaaaat tggcctgccg ggcacgcgac gggttgttgg cgcgtgggta
    10921 tggaaccgaa ataaagaaac ggtggaaacc tgccacgcaa aagcagtggt actggcaacc
    10981 ggcggtgcgt cgaaggttta tcagtacacc accaatccgg atatttcttc tggcgatggc
    11041 attgctatgg cgtggcgcgc aggctgtcgg gttgccaatc tcgaatttaa tcagttccac
    11101 cctaccgcac tgtatcaccc acaggcacgc aatttcctgt taacggaagc actgcgcggc
    11161 gaaggcgctt atctcaagcg cccggatggc acgcgtttta tgcccgattt tgatgagcgc
    11221 ggcgaactgg ccccgcgcga tattgtcgcc cgcgccattg accatgaaat gaaacgcctc
    11281 ggcgcagatt gtatgttcct cgacatcagc cataagcccg ccgattttat tcgccagcat
    11341 ttcccgatga tttatgaaaa attgctcggg ctggggattg atctcacaca agaaccggta
    11401 ccgattgtgc ctgctgcaca ttatacctgc ggtggtgtaa tggttgatga tcatgggcgt
    11461 acggacgtcg agggcttgta tgccattggc gaggtgagtt ataccggctt acacggcgct
    11521 aaccgcatgg cctcgaattc attgctggag tgtctggtct atggctggtc ggcggcagaa
    11581 gatatcacca gacgtatgcc tgatgcccac ggcgtcagta cgttaccgcc gtgggatgaa
    11641 agccgcgttg agaaccctga cgaacgggta gtaattcagc ataactggca cgagctacgg
    11701 ctgtttatgt gggattacgt tggcattgtg cgcacaacga agcgcctgga acgcgccctg
    11761 cggcggataa ccatgctcca acaagaaata gacgaatatt acgcccattt ccgcgtctca
    11821 aataatttgc tggagctgcg taatctggta caggttgccg agttgattgt tcgctgtgcg
    11881 atgatgcgca aggagagccg tggactgcat tttacgctgg attacccgga actgctcacc
    11941 cattccggtc catcgatcct ttctcccggc aatcattaca taaacagata aaaagcctgg
    12001 gtcagcgctg tatacgcttc ggaatagttc tggtctggcc cacgaatgac taagcgatca
    12061 ctaaagcatt ctcccgcctg cggggagaat gccagcagca cccgatgcgg cagtcgcgct
    12121 tcgttttccg ccacatccgt ccgcaaacgt aaatgccagc ccatgcttaa tgccagttca
    12181 gtaaaaccat taccaatctg ctctggcagc accacgcaga aaaatccctc ttcggtaatg
    12241 cactccgccg cacaggtcag cagcgatggg tgatcaagcg tagtggtata gcgagcctgt
    12301 tcccgttgag gtgtcgcaca ctctactccc tgctgatagt aaggtgggtt actgatgatt
    12361 aaatcgaagc gcgcggtctg ctgtgttacc cactgctgaa tatccgccgt atggacgtta
    12421 atccgctctg cccacgggga ctggttgata ttttcctgcg cctgcgcggc agcttcactt
    12481 tccagctcaa cggcatcaat tatcacgctg tcactggttc gctgcgccag catcagtgcc
    12541 agcaacccgc tacccgcgcc gatatcaagg caacgtttca ccccagccac cggtgcccat
    12601 gcgcccagca aaatgccatc cgttcccact ttcatcgcac agcgatcgtg agcaacaaaa
    12661 aactgtttaa aagtaaatcc attacgacga agcacggatg tagactgtga catgaaaata
    12721 aaaccttgca ggaaaaacgg cgatagcacc gggtgagaac aatacccgag aagcgatatc
    12781 catacaaaca gatgaagatt gcagccgtaa cgtctataat cagcgcccca cacagaggta
    12841 gaacatgact gtaacgactt tttccgaact tgaactcgac gaaagcctgc tggaagccct
    12901 ccaggataaa ggtttcactc gcccgaccgc cattcaggct gccgccattc cgcctgcgct
    12961 cgatggccgt gatgtactcg gttctgcgcc gacaggcacc ggtaaaacgg cggcgtatct
    13021 gctgccagcg ttgcagcacc tgctcgattt cccacgtaag aaatccggtc caccgcgtat
    13081 tttgatcctc accccaactc gcgagctggc gatgcaggtg gccgatcatg cccgcgaact
    13141 ggcgaaacat acgcatctgg atatcgccac catcaccggc ggcgtagcct atatgaacca
    13201 cgcggaagtg ttcagcgaaa atcaggacat cgtggtcgcc acgaccggac gtctgctgca
    13261 atacataaaa gaagagaact tcgattgccg cgcggttgaa acgctgatcc tcgacgaagc
    13321 agaccgtatg ctggatatgg gcttcgctca ggatatcgaa catattgctg gcgaaacgcg
    13381 ctggcgtaaa cagaccctgc tcttttcggc aacgctggaa ggcgatgcga ttcaggactt
    13441 tgccgagcgt ctgctggaag atccggtgga ggtttctgcc aatccgtcca cccgtgagcg
    13501 caaaaaaatt catcagtggt attaccgcgc cgatgacctg gaacataaaa ccgcattgct
    13561 ggtgcatctg ttaaaacagc cggaagcgac ccgctcaatt gtgtttgtgc gtaagcgtga
    13621 acgtgtgcat gagctggcaa actggctgcg cgaagcgggc atcaacaact gctatctcga
    13681 aggtgagatg gtacaaggca agcgtaacga agcgatcaag cgtttgaccg aaggtcgcgt
    13741 aaacgtactg gttgcaaccg atgttgccgc gcgcggtatc gacattcctg acgtcagcca
    13801 cgtctttaac ttcgatatgc cgcgcagtgg cgatacttat ttgcaccgta tcggacgtac
    13861 cgcgcgcgcc ggtcgtaaag gcaccgcaat ttcgctggtg gaagcccatg accatttgct
    13921 gctgggcaag gttggccgtt acattgaaga gccaattaaa gctcgcgtta ttgatgagtt
    13981 acgcccgaaa acgcgtgcgc caagcgaaaa gcagaccggc aagccatcga agaaagtact
    14041 ggctaaacgt gccgagaaga aaaaagctaa agagaaagag aagccgcggg tgaaaaaacg
    14101 ccatcgcgac accaaaaata ttggtaagcg ccgtaaacca agcggaacgg gcgtgccacc
    14161 gcaaacgaca gaagagtaat ctcaatgccg ggtttaagcc tggtattaaa agtgctggca
    14221 aacgcaaaac tgcctgatgc gctacgctta tcaggcctac gtggctcatg caatatattg
    14281 aatttgcacg atcttgtagg ccggataagg cgttcacgcc gcatccggca tgaataaagc
    14341 gcactttgtc gacaagttaa atgccgggtt taatcccgac attattttta tccagccaca
    14401 aaactctctt caacacactg aataaaagtg tgcatcgccg ggctaaccgc ttttccagca
    14461 tggtgtgcgc acatggccgt aatggtctgc gactgttcgc caaaaggcaa ttcaattaat
    14521 tctccgctct caagctcttt ttccaccgca aagcggggca agtaactgac accgatattc
    14581 gccgcgacac aacgtttgat actttcaatg cttaacagct caatggtgtt ttccaccgtg
    14641 atccgccgct gacgcagcgt gctctcaaat atctgccgga agacacattg cggttcgttg
    14701 ataataaagc tacaggcgtt atgtcttccc ggctcagtaa aatcgacatc tgcaatttgc
    14761 ggtgaagcca ccagcaccag cgattgttca cccaactctc gtcggttcag ggcatcatca
    14821 ttccctacgc gataaaagac gccgacatcg gcctcatcat tcagtagcgc atcacggatc
    14881 acgtaacagt tcagcgactg caacgataaa cgcacttttg gtgcgcgttg ccgaaaacgc
    14941 tgcaacacct gcggcattcg gtaagagagc aacgtttcgc ccgaaacaac gcgtagttcc
    15001 ccgtccggat ctgactcctt tttagcagcc tcacgaagcg tatccatcac tcgggtaagt
    15061 tcataaatgt gcggcagcaa cttttttcct tcgcgggtaa ggcacatccg tcggccaatt
    15121 ttctcaaata actggactga aaactcctgc tcaagctgct gaatatgaaa agtcaccgtc
    15181 gattgtgtac agcacaattt ttgcgaagct cgcaaaaagg aaccctcttc caccacggtt
    15241 ttaagcgtaa taaaacggcg cagatccata accccaaacc tatcgaaaat atcgaatcta
    15301 gaatataaaa acattcattt ttttaaatgt tccgtgtcgg gtactgtcta ccaaaacaga
    15361 ggagataaca agtgacaccg acccttttaa gtgctttttg gacttacacc ctgattaccg
    15421 ctatgacgcc aggaccgaac aatattctcg cccttagctc tgctacgtcg catggatttc
    15481 gtcaaagtac ccgcgtgctg gcagggatga gtctgggatt tttgattgtg atgttactgt
    15541 gtgcgggcat ttcattttca ctggcagtga ttgacccggc agcggtacac cttttgagtt
    15601 gggcgggggc ggcatatatt gtctggctgg cgtggaaaat cgccaccagc ccaacaaagg
    15661 aagacggact tcaggcaaaa ccaatcagct tttgggccag ctttgctttg cagtttgtga
    15721 acgtcaaaat cattttgtac ggtgttacgg cactgtcgac gtttgttctg ccgcaaacac
    15781 aggcgctaag ctgggtagtt ggcgtcagcg ttttgctggc gatgattggg acgtttggca
    15841 atgtatgctg ggcgctggcg gggcatctgt ttcagcaatt gtttcgccag tatggtcgcc
    15901 agttaaatat cgtgcttgcc ctgttgctgg tctattgcgc ggtacgcatt ttctattaac
    15961 gaaaaaaagc ggaagaggtc gccctcttcc gcttagtaac ttgctactta agccttacag
    16021 gctttcagta aaggtacgag cgataacgtc gcgctgctgt tccggagtca gagagttaaa
    16081 gcgaactgca taaccggata cacggatggt caactgcgga tatttttccg gatgcttaac
    16141 tgcatcttcc agagtttcac gacgcagaac gttaacgttc aggtgttgac caccttcaac
    16201 gcgaacttct ggtttcactt ctactggaac ttcacggtat tcaatgtcac ccagtttgct
    16261 tactgcaacc acttcatctt ctgcataacc tgcttttgca acgatgcaac gtgcttcgcc
    16321 tttttcgctg tccagcagcc agaaagagtt cagcagatcg tcgttagcgg ctttagtaat
    16381 ctggatacct gtaatcatgt gatgcctccc cggcaaaatt atttgatttg ttcagcctgt
    16441 cgcggccaat tggtaaaacc attgttgctt gagtgtatat atactcctca accacccttg
    16501 attctttgat ttaaatcaat aaaaaccaca catcgattgt ggtcgtaaat ggattttatt
    16561 gttttacatc aacttatgcg ggtgtgaaat tttaccaatt tacatttttt tgcactcgtt
    16621 taagtctaaa aaatgagcat gattttgttc tgtagaaaga agcagttaag ctaggcggat
    16681 tgaagattcg caggagagcg agatggctaa cgaattaacc tggcatgacg tgctggctga
    16741 agagaagcaa caaccttatt ttcttaatac ccttcagacc gtcgccagcg agcggcagtc
    16801 cggcgtcact atctatccac cacaaaaaga tgtctttaac gcgttccgct ttaccgagtt
    16861 gggtgacgtt aaagtggtga ttctcggcca ggatccttat cacggaccgg gacaggcgca
    16921 tggtctggca ttttccgttc gtcccggcat tgccattcct ccgtcattat tgaatatgta
    16981 taaagagctg gaaaatacta ttccgggctt cacccgccct aatcatggct atctcgaaag
    17041 ctgggcgcgt cagggcgttc tgctgctcaa tactgtgttg acggttcgcg caggtcaggc
    17101 gcattcccac gccagcctcg gttgggagac gtttactgat aaagtcatca gcctgattaa
    17161 ccagcaccgc gaaggcgtgg tgtttttgtt gtggggatca catgcgcaaa agaaaggggc
    17221 gattatagat aagcaacgcc atcatgtact gaaagcaccg catccgtcgc cgctttcggc
    17281 gcatcgtgga ttctttggct gcaaccattt tgtgctggca aatcagtggc tggaacaacg
    17341 tggcgagacg ccgattgact ggatgccagt attaccggca gagagtgagt aaatttgcgg
    17401 taaaatgccg gatggcagag ttgccacccg gctgatttat caggctttat tctgacgcca
    17461 ccattcacca agcaaaacgc cggttgcaac agagatattc agcccggcaa cgttgcccgt
    17521 accgtcaatc ttcacgcgca gatcgttcgg atcgcgtgcg gcatccggta acccttcata
    17581 ttcctgaccc agcaccagta ccattttcgc tggcagactg gttttgaaca gcggtttccc
    17641 ctgctcgctg gaagtggtca ctacggtgta acctgcctga cggaaatcat ccaacacgtt
    17701 aacaatgttg tcgccagtaa tcggctgaac gtgctctgcg ccgccttctg cggtacggat
    17761 agccgccccc gactccagca gtgccgcatc ctgcaccaca acgcctttca cgccgaagtg
    17821 cgcgcagctg cgcatcatgc cacccaggtt atgcgggtta gattcgtttt ccagcgccag
    17881 tacacaatcc tgcgcgcctg cctggcttac ccactgctgc acggttgtgc cattacgctt
    17941 tttgatcagg aagcaaacgc cgccgtgatg ttccgtgcct gacgcttttg tcaattccgc
    18001 ttcatccacc acatggtacg ctttgcggtt tgctgccatc cagcgcaagg cttctttaaa
    18061 acgcggcgtt acactctgga taaaccaggc gcgaacaatc gcttccggac ggctctggaa
    18121 aagtgcctga catgcatttt cgccgtagac gcgggtttct tccgcacgct gacgacgcaa
    18181 cacttccgga tcaataaaac ttttaccact gatgccaccg tgatcggcct tttccggcgt
    18241 ctcatcaccc ggcgcgcggg aaaccgtgcg ccacggcgaa tcttcccact tgcggtcacg
    18301 gggctgactc tgtttgtcat cgcgggcggg gcgacggcca ccgtcagcac gagattttcc
    18361 tggacgcccg ccccctttcc cggtacgcgg gttgtgggta cgtttatcag aatcatcatc
    18421 actgcggaca tacatcactt tgaccttgcc gcttttacct ttcatttcat cgttcatgct
    18481 tttctccacc agcgctgcgc gaagcgcgca gattacccga agtccgcgcg gttcgccatg
    18541 atttcgtacc aaagcctgcg actatcatac ctattgaata aaacagattg ttgtctggaa
    18601 caatgtcccc gataatatgt aacatattag aaacataccg gcgtcgttgc cgataagtct
    18661 ccttactcat cccgaggtta gttatgaata ccgtttgtac ccattgtcag gccatcaatc
    18721 gcattcccga cgatcggatc gaagatgcgg caaaatgcgg acgctgcggt cacgacttgt
    18781 ttgacggaga ggtgattaat gcgaccggtg aaacactcga caaattgctg aaggatgatc
    18841 tacctgtggt gatcgacttc tgggcaccgt ggtgcggccc ctgccgtaat ttcgcaccaa
    18901 tttttgaaga tgtcgcgcaa gagcgtagcg gtaaagtgcg ctttgtgaaa gtgaataccg
    18961 aagctgaacg tgaattgagc agtcgctttg gaattcgtag tataccgacg atcatgattt
    19021 tcaaaaacgg tcaggttgtc gacatgctta atggcgcagt accgaaagcg ccgttcgata
    19081 gctggctgaa cgaatctctt taatcttacc ggggcgcatc ttgtgccccg ttttctcctc
    19141 tgcgacaatg gcgttttttc gacgctctct tatgaccgaa aacgctgttc tccagttacg
    19201 cgccgagcgt attgcgcgcg caacacgtcc ttttcttgcc cgcggtaatc gcgttcgtcg
    19261 ctgccaacgc tgtcttttgc cagagaaatt atgtctctgt tcgacaatta ccccagcaca
    19321 agcaaaaagt cgcttctgtt tgctgatgtt cgacaccgag ccaatgaagc ccagtaatac
    19381 cgggcgtctg attgctgata ttttgcctga taccgttgcg tttcaatggt cgcgtaccga
    19441 accctcgcaa gatttactgg atctggtaca aaacccggac tatcagccaa tggtggtctt
    19501 tcccgcttcg tatgctgatg agcaacggga agtgatcttc acaccacctg ccggtaagcc
    19561 accgctgttt atcatgctcg atggtacctg gccggaagct cgcaagatgt ttcgtaaaag
    19621 tccgtatctg gataatcttc ccgtcatttc cgtcgatctt tcccggcttt ctgcctatcg
    19681 cctgcgtgaa gcccaggctg aaggccaata ttgtactgcc gaggtagcca tcgcactgtt
    19741 agatatggcc ggcgataccg gggcggcagc agggttaggc gagcatttta cccgctttaa
    19801 aacacgctat ctggcaggaa aaacgcaaca tctgggtagc atcacagcag aacagttaga
    19861 aagcgtttaa aatcattcgg tcacttctgc gggagaccgg tatgagtcag cgaggactgg
    19921 aagcactact gcgaccaaaa tcgatagcgg taattggcgc gtcgatgaaa cccaatcgcg
    19981 caggttacct gatgatgcgt aacctgctgg cgggaggctt taacggaccg gtactcccgg
    20041 tgacgccagc ctggaaagcg gtgttgggtg tgttggcctg gccggatatt gccagcttgc
    20101 cctttacacc cgaccttgcg gttttatgta ccaatgccag ccgtaatctt gctcttctgg
    20161 aagagctcgg cgagaaaggc tgtaaaacct gcattattct ttccgccccg gcatcgcaac
    20221 acgaagatct ccgcgcctgc gccctgcgcc ataacatgcg cctgcttgga ccaaacagtc
    20281 tgggtttact ggctccctgg caaggtctga atgccagctt ttcgcctgtg ccgattaaac
    20341 gcggcaagct ggcgtttatt tcacaatcgg cagccgtctc caacaccatc ctcgactggg
    20401 cgcaacagcg tgagatgggc ttttcctact ttattgcgct cggcgacagc ctggatatcg
    20461 acgttgatga attgctcgac tatctggcgc gcgacagtaa aaccagcgcc atcctgctct
    20521 atctcgaaca gttaagcgac gcgcgacgct ttgtttcggc agcccgtagt gcctcgcgta
    20581 ataaaccgat tctggtgatt aaaagcggac gtagcccggc ggcacagcga ctgctcaaca
    20641 cgacggcagg aatggacccg gcatgggatg cggctattca gcgtgccggt ttgttgcggg
    20701 tacaggatac ccacgaactg ttttcggcgg tggaaaccct tagccatatg cgcccgctgc
    20761 gtggcgaccg gttgatgatt atcagcaacg gtgctgcgcc tgccgcgctg gcgctggatg
    20821 ccttatggtc acgcaatggc aagctggcaa cgctaagcga agaaacctgc cagaaactgc
    20881 gcgatgcact gccaggacat gtggcaatct ctaacccgct cgatctgcgc gatgacgcca
    20941 gcagtgagca ctatattaaa acgctggaca ttctgctcca cagccaggat tttgatgcgc
    21001 tgatggttat tcattcgccc agcgccgctg ctcccgcaac agaaagcgcg caagtattaa
    21061 ttgaagcggt aaagcatcat ccccgcagca agtatgtttc tctgctgacg aactggtgtg
    21121 gcgaacattc ctcgcaagag gcacgacgat tattcagcga agccgggctg ccgacctacc
    21181 gtaccccgga aggaaccatc actgctttta tgcatatggt ggagtaccgg cgtaatcaga
    21241 agcaactacg cgaaacgccg gcgttgccca gcaatctgac ctccaatacc gcagaagcgc
    21301 atcttctgtt gcaacaggcg attgccgaag gagcaacgtc actcgatacc catgaagttc
    21361 agcctatcct gcaagcatat ggcatgaaca cgctccccac ctggattgcc agcgatagca
    21421 ccgaagcggt gcatattgcc gaacagattg gttatccggt ggcgctgaaa ttgcgttcgc
    21481 cggatattcc acataaatcg gaagttcagg gcgtcatgct ctacctgcgt acagccaatg
    21541 aagtccagca agcggcaaac gccattttcg atcgcgtaaa aatggcctgg ccgcaggcac
    21601 gggtccacgg cctgttggtg caaagtatgg ctaaccgtgc tggtgctcag gagttgcggg
    21661 ttgtggttga gcacgatccg gttttcgggc cgttgatcat gctgggcgaa ggcggtgtgg
    21721 agtggcgtcc tgaagatcaa gccgtcgtcg cgttaccgcc gctgaacatg aacctggccc
    21781 gctatctggt tattcagggg atcaaaagta aaaagattcg tgcacgcagt gcgctacgcc
    21841 cattggatgt tgcaggcttg agccagcttc tggtgcaggt ttccaacttg attgtcgatt
    21901 gcccggaaat tcagcgtctg gatattcatc ctttgctggc ttctggcagt gaatttaccg
    21961 cgctggatgt cacgctggat atctcgccgt ttgaaggcga taacgagagt cggctggcag
    22021 tgcgccctta tccgcatcag ctggaagaat gggtagaatt gaaaaacggt gaacgctgct
    22081 tgttccgccc gattttgcca gaagatgagc cacaacttca gcaattcatt tcgcgagtca
    22141 ccaaagaaga tctttattac cgctacttta gcgagatcaa cgaatttacc catgaagatt
    22201 tagccaacat gacacagatc gactacgatc gggaaatggc gtttgtagcg gtacgacgta
    22261 ttgatcaaac ggaagagatc ctcggcgtca cgcgtgcgat ttccgatcct gataacatcg
    22321 atgccgaatt tgctgtgctg gttcgctcgg atctcaaagg gttaggctta ggtcgacgct
    22381 taatggaaaa gttgattacc tatacgcgag atcacggact acaacgtctg aatggtatta
    22441 cgatgccaaa caatcgtggc atggtggcgc tagcccgcaa gctcgggttt aacgttgata
    22501 tccagctcga agaggggatc gttgggctta cgctaaatct tgcccaacgc gaggaatcat
    22561 gagtaaggta ctggaaatgt tgaccactta atcgggactg gtgttattat tgcccgctta
    22621 tgtcgtctgc attgcacaga ggacccttca atgaacagag aagaaatgca ctgtgatgtt
    22681 gtcaaaattt aagcgtaata aacatcaaca acaccttgcc caactaccca agatttctca
    22741 atcagttgat gatgtcgatt tcttttacgc tcccgccgac ttccgggaga cgctgctgga
    22801 aaaaatagcc agcgcgaagc agcgcatttg cattgtcgcc ctgtatctcg aacaggatga
    22861 cggtggcaaa ggcattctga acgcgttgta tgaagctaaa aggcagcgtc cggaactgga
    22921 tgtgcgggtg ctggtcgact ggcatcgtgc acaacgtgga cgcattggcg ctgcggcatc
    22981 taacactaac gctgactggt actgccgcat ggcgcaggaa aatccgggcg tagatgttcc
    23041 ggtttatggc gttccaatca atacccgtga agcccttggt gttctgcact ttaaaggctt
    23101 tatcatcgac gatagcgtac tttatagcgg tgccagcctg aacgatgttt acctgcatca
    23161 gcacgataaa tatcgctacg accgttatca tcttatccgt aaccgtaaga tgtcagacat
    23221 tatgtttgaa tgggttacac agaatattat gaatggccgc ggcgttaatc gtctggatga
    23281 tgttaatcgg ccaaaaagcc cggaaatcaa gaacgatatt cgtctgttcc gccaggagct
    23341 gcgtgatgcc gcttatcatt tccagggcga tgccgacaac gatcagcttt ccgtaacgcc
    23401 gctagtgggg ctggggaaat cgagtctgtt gaacaagacc attttccatc ttatgccttg
    23461 tgccgagcag aaactaacca tctgtacgcc atacttcaac ctgccagcaa tccttgtgcg
    23521 caatattatc cagttgctgc gcgaagggaa aaaggtcgaa attattgttg gtgataaaac
    23581 cgcgaacgac ttctacattc cggaagatga acctttcaag ataattggcg cattgcctta
    23641 tctctatgag atcaatctgc gtcgtttcct gagccgtttg cagtattacg tcaatactga
    23701 ccagctagtg gttcggttat ggaaagatga cgacaacacc tatcacctaa aagggatgtg
    23761 ggttgatgat aagtggatgt tgatcaccgg taataacctg aacccgcgcg cctggcgtct
    23821 ggatctggaa aacgccattt tgatccacga tccacaactg gagcttgcgc cgcagcgaga
    23881 gaaagaactg gagctgatcc gcgagcatac caccatcgtt aagcactatc gcgatctgca
    23941 aagtattgcc gattatccgg tgaaggttcg taaactcatc cgccgtttgc gccgtatccg
    24001 catcgaccga ttaattagcc gcatcctgta atcacaaccc cgtcctgtac ggggtttgtt
    24061 ttttggaggc cacgttttgc gtattctttt tgtctgttcg ctattgttac tttctggatg
    24121 cagccatatg gctaacgata gctggagcgg gcaggataaa gctcaacact ttatcgcctc
    24181 ggcgatgctt tccgccgccg gaaatgaata ttcacagcat caggggatga gccgggatcg
    24241 cagtgccatg tttggattga tgttctctgt cagtttgggg gcgtcaaaag agctttggga
    24301 tagccgcccc gaagggagcg gctggagctg gaaggatttg gcctgggatg tcgccggtgc
    24361 aagcaccggc tataccgtct ggcaactgac ccgtcactaa agacgcatcc ccttcccttt
    24421 gcgatgtagc atcaaagaaa ccagaaacgc caccacggcc atcaaggtca cataccagaa
    24481 gaaggctgtt tccattccta ttgatttcag cgacaacgct acgtactccg ccgaaccacc
    24541 aaatatagca ttagcgaccg catatgacag gccaacgcct aatgcgcgaa cctgtgccgg
    24601 gaacatctca gccttcagta ttccactgat tgatgtataa aaactcacta tcagcagggc
    24661 acacatcacc agaccaaaag cggcataagg cgaggaaacg ttttgcaatg ctgagagaat
    24721 aggaacggta aaaatggctg ccagcgaacc gaaacataac attgaggtac ggcgaccaat
    24781 cttatccgac agcgcgccaa tgagtggttg aataagcatg aatacaaaca atgcggcagt
    24841 cataatgcca ctcgccacgt tggcatgcat tcccgcagta tttaccagat acttctgcat
    24901 ataagtggtg aaggtataga aacaaaggga gcccgcagcg gtaaaaccga gaaccatgat
    24961 gaatgcacgg cgattgcgcc ataatccttt cagagatcca gcttctttta aagcgcgcgt
    25021 ttcttgttgc gaagtttcat ctaattgacg acgtaaccac aacgccacaa cagctaacac
    25081 agctcctaac gcgaaaggaa tacgccatcc ccactctctg agtgcagcgt cttccatggt
    25141 gtgttgtaaa accacgacaa ccagtagggc tagcagttgt ccgccgatca acgtcacata
    25201 ctgaaatgat gcgtaaaaac ctttgcgccc ttcaacggca acttcactca tataggtggc
    25261 gctggtgcca tattctccgc caacggataa tccctgaaat aaacgagcga gaagcaataa
    25321 tgccggagcc cacgtaccta tagtttcata acctgggagg caggcgataa ccagcgatcc
    25381 gaaacacatc atacacaccg ataacagcat cgattttttg cgaccatgtt tatcggctat
    25441 gcggccaaat agccaaccgc ctattgggcg catcaggaat cccgcagcaa aaacacctgc
    25501 tgtttgtagt agttgagtcg tcgtgttccc ggaagggaag aaaatgtggg cgaagtagag
    25561 tgaacagaac gagtagacat agaaatcgaa ccactcgacc agattacctg aagaggcccc
    25621 cacaatcgcc caaatgcggc gacgagtatc actacttgtc agtttgctgt ctgccgttac
    25681 agtactttca gccatgccat tatgtctcct gccgtaatcc gatgcttttg tcggtcgctt
    25741 ttatttattt cttttggtaa agaaaatgtt atacatttgt tgcgtatcat tatgcaacct
    25801 taaccatgaa tttagttagc aggaaaatgg ttattgagga gcttaaggat aaatttctgg
    25861 taaggaggac acgtatggaa gtaggcaagt tggggaaacc gcatccgttg ctgaatctgg
    25921 catatgtggg agtataagac gcgcagcgtc gcatcaggca tttttttctg cgccaatgca
    25981 aaaaggccat cctgacggat ggccttt
//
LOCUS       DBANUU010000061        25518 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000061 DBANUU010000000
VERSION     DBANUU010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.061"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            221..640
                     /locus_tag="ACIE68_002480"
     CDS             221..640
                     /locus_tag="ACIE68_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFX5908787.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            640..1908
                     /gene="umuC"
                     /locus_tag="ACIE68_002481"
     CDS             640..1908
                     /gene="umuC"
                     /locus_tag="ACIE68_002481"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFX5908788.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALSVDEVWGIGRRNSKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1954..2484)
                     /gene="dsbB"
                     /locus_tag="ACIE68_002482"
     CDS             complement(1954..2484)
                     /gene="dsbB"
                     /locus_tag="ACIE68_002482"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFX5908789.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2630..4171)
                     /gene="nhaB"
                     /locus_tag="ACIE68_002483"
     CDS             complement(2630..4171)
                     /gene="nhaB"
                     /locus_tag="ACIE68_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFX5908790.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4393..5112
                     /gene="fadR"
                     /locus_tag="ACIE68_002484"
     CDS             4393..5112
                     /gene="fadR"
                     /locus_tag="ACIE68_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFX5908791.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5164..6696)
                     /locus_tag="ACIE68_002485"
     CDS             complement(5164..6696)
                     /locus_tag="ACIE68_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFX5908792.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7026..8324
                     /gene="dadA"
                     /locus_tag="ACIE68_002486"
     CDS             7026..8324
                     /gene="dadA"
                     /locus_tag="ACIE68_002486"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFX5908793.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8334..9404
                     /gene="dadX"
                     /locus_tag="ACIE68_002487"
     CDS             8334..9404
                     /gene="dadX"
                     /locus_tag="ACIE68_002487"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFX5908794.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9790..11526)
                     /locus_tag="ACIE68_002488"
     CDS             complement(9790..11526)
                     /locus_tag="ACIE68_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFX5908795.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11621..12535)
                     /gene="ldcA"
                     /locus_tag="ACIE68_002489"
     CDS             complement(11621..12535)
                     /gene="ldcA"
                     /locus_tag="ACIE68_002489"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFX5908796.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIHRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12635..13246
                     /gene="emtA"
                     /locus_tag="ACIE68_002490"
     CDS             12635..13246
                     /gene="emtA"
                     /locus_tag="ACIE68_002490"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFX5908797.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13248..13982)
                     /locus_tag="ACIE68_002491"
     CDS             complement(13248..13982)
                     /locus_tag="ACIE68_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFX5908798.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14183..14437
                     /locus_tag="ACIE68_002492"
     CDS             14183..14437
                     /locus_tag="ACIE68_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFX5908799.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            14615..15055
                     /gene="ycgY"
                     /locus_tag="ACIE68_002493"
     CDS             14615..15055
                     /gene="ycgY"
                     /locus_tag="ACIE68_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFX5908800.1"
                     /translation="MIQKINILDISQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15134..16831)
                     /gene="treA"
                     /locus_tag="ACIE68_002494"
     CDS             complement(15134..16831)
                     /gene="treA"
                     /locus_tag="ACIE68_002494"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFX5908801.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSYKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17151..18569)
                     /gene="dhaM"
                     /locus_tag="ACIE68_002495"
     CDS             complement(17151..18569)
                     /gene="dhaM"
                     /locus_tag="ACIE68_002495"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFX5908802.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLLSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELEIGWICQQGEKL
                     YAIQPEEKLTLDVKTQRFNRQG"
     gene            complement(18580..19212)
                     /gene="dhaL"
                     /locus_tag="ACIE68_002496"
     CDS             complement(18580..19212)
                     /gene="dhaL"
                     /locus_tag="ACIE68_002496"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFX5908803.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESGYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19223..20293)
                     /gene="dhaK"
                     /locus_tag="ACIE68_002497"
     CDS             complement(19223..20293)
                     /gene="dhaK"
                     /locus_tag="ACIE68_002497"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFX5908804.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQEPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQQLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20521..22440
                     /gene="dhaR"
                     /locus_tag="ACIE68_002498"
     CDS             20521..22440
                     /gene="dhaR"
                     /locus_tag="ACIE68_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFX5908805.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22540..25425)
                     /locus_tag="ACIE68_002499"
     CDS             complement(22540..25425)
                     /locus_tag="ACIE68_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFX5908806.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTSGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVASGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDPVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHSGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTNNSNLDTLALSHSTVDFAS
                     HASTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPKPTPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVR
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTEYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYHFSF"
BASE COUNT         6285 a         6347 c         6584 g         6302 t
ORIGIN      
        1 atgtcattat ggcgaatgct tctattctat tttttagccg ggtgatattt ttcatttctg
       61 ctggatgcgt cgtcgccaga aggccacgtg agcacaggat aagagaacga aaaatcagca
      121 gtctatgcag cgacaaatat tgatagcctg aatcagtatt gatctgctgg caagaacaga
      181 ctactgtata taaaaacagt ataacttcag gcagattatt atgttgttta tcaagcctgc
      241 ggatctccgc gaaattgtga cttttccgct atttagcgat cttgttcagt gtggctttcc
      301 ttcaccggcc gcagattacg ttgaacagcg catcgatctg aatcaactgt tgatccagca
      361 tcccagcgcg acttatttcg tcaaagcaag tggtgattct atgattgatg gtgggattag
      421 tgacggtgat ttactgattg tcgatagcgc tattaccgcc agccatggtg atattgtcat
      481 cgctgctgtt gacggcgagt ttacggtgaa aaaattgcaa ctacgcccga cggtacagct
      541 tattcccatg aacagcgcgt actcgcccat taccatcagt agtgaagata cgctggatgt
      601 ctttggtgtg gtgatccacg tcgtaaaggc gatgcgctga tgtttgccct ctgtgatgta
      661 aacgcgtttt atgccagctg tgagacggtg tttcgccctg atttatgggg taaaccggtg
      721 gttgtgctat cgaataatga cggttgcgtt atcgcccgaa acgctgaggc aaaggcgctt
      781 ggcgttaaaa tgggcgatcc ctggttcaaa caaaaagatc tgtttcgtcg ctgtggcgtg
      841 gtttgcttta gcagcaatta tgagctttac gcagacatga gcaatcgggt gatgtcgacg
      901 ctggaagaat tatcgccccg cgtcgagatt tacagtattg atgaggcatt ctgcgatctg
      961 acaggtgtgc gtaattgtcg cgatctgact gattttggca gagaaattcg cgcaacggtg
     1021 ctacaacgta cccatcttac tgttggtgtg gggatcgccc agaccaaaac gctggctaag
     1081 cttgccaatc atgcggcaaa aaaatggcag cggcagacgg gtggggtggt ggatttatca
     1141 aatctggaac gccagcgtaa attaatgtct gctctctccg tggatgaagt ctgggggatt
     1201 ggacggcgga acagcaaaaa actggacgcg atggggatca aaaccgttct cgatttggcg
     1261 gatacagata tccggtttat ccgtaaacat tttaatgtcg tgctcgaaag aacggtgcgt
     1321 gaactgcgcg gcgaaccctg tttgcaactg gaagagtttg caccgacgaa gcaggaaatt
     1381 atctgttccc gctcgtttgg tgaacgcatc acggattata cgtcgatgcg gcaggccatt
     1441 tgtagttacg ctgcccgggc ggcggaaaaa cttcgcagcg agcatcaata ttgtcggttt
     1501 atctccacat ttattaagac gtcaccattt gcgctcaatg aaccttatta cggcaatagc
     1561 gcgtcggtaa aactgctgac gcccactcag gacagcaggg atatcattaa cgccgctacg
     1621 cgatctctgg atgccatctg gcaagcaggc catcgctacc aaaaagcggg cgtgatgctg
     1681 ggggatttct tcagtcaggg agtcgcgcag ctcaatttat tcgatgacaa cgcaccgcgc
     1741 cccgggagtg agcaattgat ggcggtaatg gatacactga atgctaaaga gggcagagga
     1801 acactctatt ttgccgggca ggggatccag caacaatggc agatgaagcg agccatgctt
     1861 tcaccacgtt atacaacgcg aagttctgat ttactgcggg tcaaataaac atagcggcag
     1921 gaaaaaagcg ctcccgcagg agcgccgaat ggattagcga ccgaacaggt cacgtttttt
     1981 cgctttaaac ggctgggaaa tcaccaccag caccgcgaca atcaggtaag cgataaaaat
     2041 accgagcagc cactgcggca tttccagacc taaaaaatcc cactgacgct cggcacaatc
     2101 gccagaggcg acaaacactt gcggcaccca tttatccagc ggcagccatt ccgggaaacg
     2161 aaccataaaa tcacaggtgg caaacggcga aggatagagc tgaagcatgg tgtgctcgta
     2221 tgttaactgc acaccgcgga acgcactata caaccagata accatcgcta cataacgcag
     2281 tggcgttttc ggggcgatcg cgccaatcag cgcagcaccc agaacgccga ataatgcgca
     2341 gcgttcataa atacagagca cgcaaggttt cagtaacatc acatgctgga accacagcgc
     2401 cgtcagttcc agtgccagag cagtaaacgc catcaacagc cacgcgcccc ggccttgtga
     2461 acattggttc aaaaatcgca acataatcat ttccctgcaa tatgcataga gtgcgcagtt
     2521 taaaccaatt cattcgctgc gccaccagga ggcagcaaaa ataggacgca attggacaat
     2581 tatcgggcga aaaggcaaac cgggcattta tatgcccggt aagttgttat caaagcgttg
     2641 ctatccagcc catttgcata aaccattcgg ttacaggggc aagcgtaaac tcgacgcaga
     2701 gcagtccgac gagtgtcagt acgagggtgt aaggcagggc catccacacc atgcggccat
     2761 aagagaggcg aatcaatggc gcgagtgcag aggtcagcag gaacaggaac gcagcctgac
     2821 cgttcggcgt agcgacggag ggcagattgg taccggtatt aatggcgacc gccagcagct
     2881 cgtattgctt caacgtgata gcgccacttt ccattgccgc tttcgcttcg ttgatataaa
     2941 tcgtccccac gaagacgtta tccgaaatgg atgacagcag accgttgaaa atatagaaca
     3001 gcgacagctg agcatgttcc gatgcctgca acacaaactg aataattggc gaaaacagtt
     3061 gttggtcgat aatcaccgcg actaccgaga aaaacaccgt caacagtgcg gtgaatggca
     3121 gagattcggt gaaggcttta ccgatagcat gctcatcggt gacaccggtc aatgatgttg
     3181 ccagaataat gactgacaaa ccaatcaagc caacttccgc cagatgcaac gccagcgcag
     3241 tcaccagcca gacgccaata atcgcctgga caatcagacg gattttatcc tgacgggtac
     3301 gctggtggcg gctttgatcg tcaaactgtt gcaacacttc gcggactttc tccggcagcg
     3361 tttcaccgta gccaaaccaa cgcagcttct ctaccagcag gcaggttaac aggccacaaa
     3421 tcagaaccgg aacggtcacc ggcgacatgc gcaggaagaa atcgccaaaa tgccagccag
     3481 ccgctttagc gatgatcagg ttctgtggtt cgcccaccat ggtcattacg ccgcctaatg
     3541 cggtgccgac accggcatgc atcatcaggc tacgcagaaa gccacggaac tgttccagaa
     3601 ccactttgta atgcttgtcg atatgactat cgtcttgcag gtcggtgtct tcggtacggg
     3661 aagaggctac gcgatgataa ataccataaa aaccgactgc aacgctgatc accaccgcca
     3721 cgacggttaa ggcatcgagg aacgcggaga ggaacgcagc cgccacgcaa aaagagagcg
     3781 acagcagcat tttggagcga atgcttaaca gcaaacgggt aaatatgaac agcaacagct
     3841 gtttcataaa atagataccc gccaccataa acatcagtaa cagcaagact tcaagatttg
     3901 ccgccacctc ttcacggacg tgttccgcgc tggtcatgcc gatgaatacc gcttcgatag
     3961 ccaacagacc accggggagc agcgggtagc atttcagggc catcgccaga gtgaaaataa
     4021 attccgctac cagcaaccag cccgcgacga aagggctgat gaggaaaatt aacggattta
     4081 cgattaagaa aatgatgagg gcgagtttgt accagtcggg ggactggccc aaaaaattgc
     4141 gccatagcgc gcggccccag gagatctcca tgatggtttc ccttacctta caaataatca
     4201 atgatgtttt tatgtttaaa cgcaaagctt aacggtcagg caggagtgag gcaagtcttg
     4261 atggtcaagg ggaaagagat gcggaaaatg aagccttgat cccttttttc ttctttttgt
     4321 ctgctatcag cgtagttagc cctctggtat gatgagtcca actttgtttt gctgtgttat
     4381 ggaaatctca ctatggtcat taaggcgcaa agcccggcgg gtttcgcgga agagtacatt
     4441 attgaaagta tctggaataa ccgcttccct cccgggacta ttttgcccgc agaacgtgaa
     4501 ctttcagaat taattggcgt aacgcgtact acgttacgtg aagtgttaca gcgtctggca
     4561 cgagatggct ggttgaccat tcaacatggc aagccgacga aggtgaataa tttctgggaa
     4621 acttccggtt taaatatcct tgaaacactg gcgcgactgg atcacgaaag tgtgccgcag
     4681 cttattgata atttgctgtc ggtgcgtacc aatatttcca ctatttttat tcgcaccgcg
     4741 tttcgtcagc atcccgataa agcgcaggaa gtgctggcta ccgctaatga agtggccgat
     4801 cacgccgatg cctttgccga gctggattac aacatattcc gcggcctggc gtttgcttcc
     4861 ggcaacccga tttacggtct gattcttaac gggatgaaag ggctgtatac gcgtattggt
     4921 cgtcactatt tcgccaatcc ggaagcgcgc agtctggcgc tgggcttcta ccacaaactg
     4981 tcggcgttgt gcagtgaagg cgcgcacgat caggtgtacg aaacagtgcg tcgctatggg
     5041 catgagagtg gcgagatttg gcaccggatg cagaaaaatc tgccgggtga tttagccatt
     5101 caggggcgat aatcccttcc gtttaaagag caaacccctc aaacgagggg ttttttgttg
     5161 tttttacaga tttcccattc ttggcgggca acgttccagc aactcgatgc tgccgtcttc
     5221 gttttgctgt tcgagcatca catcaaatcc ccacaggcga tgcacatgct tcagcacttc
     5281 tttgcgcccc cgatccagcg gtgcgcgatt atgtggaatg taacgcagcg tcagcgaacg
     5341 gtcgccgcgc aaatccacgt tccagatctg aatattcggc tccagattac ttaagttata
     5401 ttgcgacgat aaccggttac ggatctcccg ataaccttct tcattatgaa tagcggaaat
     5461 ctccagataa ttatgccgat catcatccag cacggtgaag aagcggaaat cacgcatcac
     5521 tttcggtgac agaaactggc tgataaaact ctcatctttg aaatcacgca tcgcgaaatg
     5581 cagcgtttcc agccagtcgg aaccggcgat atccgggaac cagtatttgt cttcttccgt
     5641 tggcgactga caaatccgtt taatatcctg gaacatggcg aacccgaggg catatgggtt
     5701 gatgccgctg taccacgggc tgttataggg gggctggaag accacattgg tgtggctgtg
     5761 caaaaactcc agcataaaac gttccgttac tttcccttca tcatacagat ggttaaggat
     5821 ggtatagtgc cagaaggtcg cccagccttc gttcatcacc tgagtctgtt tttgcggata
     5881 aaaatactgg ctcaccttac gcacaatacg caggatttca cgctgccatg attccagcag
     5941 tggcgcattt ttctccataa aatagagcag gttttcttgt ggttcggacg gatagcggcg
     6001 cgcttcagca accgttttct cttcctcgcg cttcggcagg gtacgccaga gcatattgac
     6061 ctgactttgt agatactctt cgcgactttt ctgccgggct ttctcttctt gcagcgagat
     6121 tttttgcggg cgtttgtacc ggtccacgcc gtagttcatc agcgcgtggc acgagtccag
     6181 aagccgttct acttcatcaa cgccataacg ttcttcgcac tcggtaatat atttacgggc
     6241 aaaaatcaga taatcgacaa tcgaactggc gtcggtccaa ctgcggaata agtaattgtt
     6301 tttaaagaaa gagttatgcc catagcaggc gtgtgccatc accagcgcct gcatggtaat
     6361 ggtgttctct tccatcaggt aagcgataca cgggttagag ttaatgacga tttcataggc
     6421 cagtccttgc tgaccgtgct tatacagccg ttcagtctcg ataaactttt taccgaatga
     6481 ccagtgcgga tagttaattg gcatgccgac gctggagtag gcatccatca tctgttctga
     6541 ggttatcact tcaatctggt gcgggtaggt atccagccgg tagagtttcg ccacccggtc
     6601 tatctctgcc agataaacat ccagcaggtc gaacgtccag tcgggtccat cgctcaaacg
     6661 tgtggtgtcc ttattcatag aatcgatcgt cgccatacgc gcacctcatt gttgtcggcg
     6721 ctctctgtgt ggagcacctc atttcaagca tagaacacct gttaaaaacc gcgtcgccgg
     6781 agaatttttt tctttgcgat ttcttattat cagagtgcca ctaatccgct tctgaacgga
     6841 attttatgct ggataaaaag ggcgttcagc aggagatact aaagacgcca tattgccgca
     6901 gagtcaggga gatgtgagcc agctcaccat aaaaaagccg tatgttgaat aatattttca
     6961 actgagttat caagatgtaa ttagattatt attcttttac tgtatctacc gttatcggag
     7021 tggctatgcg agttgtcata ctgggaagtg gtgtggtagg cgttgccagc gcctggtact
     7081 taaatcaggc aggacatgag gtcaccgtca ttgatcggga gccgggggcc gccctggaaa
     7141 ccagcgccgc gaatgccggg caaatctccc ccggatatgc tgcaccgtgg gcggcaccag
     7201 gtgtgccttt aaaagcgatt aaatggatgt tccagcgcca tgcgccgctg gcggttcgtc
     7261 tcgacggtac gcagttccag ttgaaatgga tgtggcaaat gttacgtaac tgcgacacca
     7321 gccactacat ggaaaacaaa gggcggatgg tgcgtctggc ggaatacagc cgtgattgcc
     7381 tgaaagcatt gcgcgccgaa accaatattc agtatgaagg gcgtcagggt gggacgctgc
     7441 aactgttccg taccgaacaa cagtatgaaa atgcgacccg cgatatcgcc gtgctggaag
     7501 atgccggcgt accgtatcag ctgctggaat ccagccgcct ggcggaagtg gagcccgcgc
     7561 tggcagaagt agcgcacaaa ctgacgggtg gtctgcaact gcccaatgat gaaaccggag
     7621 actgccagct gtttacccag aatctggcgc ggatggcaga gcaggcgggg gttaaattcc
     7681 gctttaatac gcccgttgac caactgcttt gcgacggcga gcaaatctac ggcgtgaagt
     7741 gtggcgatga agtgattaag gccgatgcgt atgtgatggc gtttggttct tactcgacgg
     7801 cgatgctcaa aggcattgtt gatattccgg tgtatccgct gaaaggctac tcgctgacta
     7861 ttccaattgc gcaggaagat ggtgcgccag tatccaccat tcttgatgaa acctacaaaa
     7921 tcgccattac ccgtttcgat aaccgcattc gtgttggcgg aatggcggaa attgttggtt
     7981 ttaacaccga gctgttgcaa ccgcgtcgtg aaacgctgga aatggtggtt cgcgatctct
     8041 atccacgcgg cggtcatgtc gagcaggcga ctttctggac gggtttgcgc ccgatgacgc
     8101 cagacggtac gccggttgtc gggcgtacac gctttaaaaa tctgtggctg aataccggtc
     8161 acggcacgct cggctggacg atggcttgcg gttccggtca gttgttaagc gatctgctct
     8221 ctggtcgcac gccagcgatc ccatatgagg atctaagcgt agcgcgctac agccgtggat
     8281 ttacgccatc acgtccgggc catttacatg gcgcacacag ctaaggaaac gagatgaccc
     8341 gtccgataca ggccagcctc gatctgcagg cattaaaaca gaatctgtcc attgtccgcc
     8401 aggccgcgcc gcacgcgcgc gtctggtcgg tggtaaaagc gaacgcttac gggcatggta
     8461 ttgagcgtat ctggagcgcg ctcggggcca ccgatggctt tgcattactt aacctggaag
     8521 aggcaataac gttacgtgag cgcggctgga aggggccgat cctgatgctg gaaggatttt
     8581 tccatgctca ggatctggag atttatgacc agcaccgcct gaccacctgc gtacacagca
     8641 actggcagct caaagcactg caaaatgcgc ggctaaaagc accgttggat atttatctta
     8701 aagtgaacag tgggatgaat cggttgggct tccagcccga tcgcgtgctt accgtctggc
     8761 agcagttgcg ggcaatggcg aatgttggcg agatgaccct gatgtcgcat tttgccgaag
     8821 cggaacatcc tgatggaatt tccagcgcga tggcgcgtat tgagcaggcg gcggaagggc
     8881 tggagtgtcg gcgttcgttg tccaattcgg cggcgactct gtggcacccg gaagcgcatt
     8941 ttgactgggt tcggcctggc attattttgt atggcgcttc gccgtccggt cagtggcgtg
     9001 atatcgccaa taccggatta cgtccggtga tgacgctaag cagtgagatt attggtgtcc
     9061 agacgctaaa agcgggtgag cgtgtgggct acggcggtcg ctatactgcg cgcgatgaac
     9121 agcgaatcgg cattgtcgcc gcagggtacg ccgacggtta tccgcgccac gcgcctaccg
     9181 gtacccctgt tttagtggac ggcgtgcgca ccatgacggt ggggaccgtc tcgatggata
     9241 tgctagcggt cgatttaacg ccttgcccgc aggcggggat tggtacgccg gttgagctgt
     9301 ggggcaagga gatcaaaatt gatgatgtcg ccgccgctgc cggaacggtg ggctatgagt
     9361 tgatgtgcgc gctggcgtta cgcgtcccgg ttgtgacagt gtaacttgtt gtaagccgga
     9421 tcggagacag cgtcttctgg ttgcgaaaaa atcatccatc cggctggtca gcaactgtag
     9481 ttgttaatgt gacagagcca ttgcccatga tagtgtccat taaaagaatg gacactattt
     9541 ccccggaacc tgaactcacc gcacaggcgt tctacataaa acgcttacgc ttcattgttg
     9601 actcgaactc gacttcagat aaatcacgct ttacccttga tggagcctgt acatagattt
     9661 gtgtaattgc ctgattttga tatgttcaat tcaacatcaa atgaaggtta aattatggac
     9721 gacaaacaat tgcaggctca ggctgcgttc agcaaagcat cgcaaccggc gatagatgct
     9781 tcattaaatt taagattcag cttcctcttc agccacccgt acgccaatct tcaacacttc
     9841 attatcttct ttctcggcca ccgtccagat catcccggca aactctacct ggtcaccgac
     9901 aaccggtgcc gcgcctaaca actgctggac aatttcaccc agcgtttgct gcttatcacg
     9961 atattctcgc ccgtcttcca ggccatatat cagcgcaaca tcagcatatt tggcgctggc
    10021 ttcgagaatg aagtcaccaa agaagcgttg atccagcgcg accggcggcg actggctgaa
    10081 cagtttaccg agcgccggaa gatcgcgttc ccgaccaatt acacacaaca catcgccttc
    10141 gcgcagtcgg gtgctgccgg taggatgaag caactggtta tcacgaaaca gtgccgcaat
    10201 acgcgtctct tttggcatat gcaaatcgcg cagtgccgcg cccacgcacc atttatcggc
    10261 actcaattga taaacaaact gctcccacgg attttccgga tgaatatcca ggccaacgcg
    10321 tgacaccgga cgtcccactg gcggaaccac cactttggct tttttagccg cccacgagag
    10381 tgatgttccc tgcaagagca gtgaaaccag aaccacaaag aaggcgacat taaagaacag
    10441 acgtgcattc tccagccccg ccatcatcgg gaacacggcc aggatgattg gcaccgcgcc
    10501 gcgtaatcct acccagctga taaacacgcg ctcacgcaga ttgaaaccgc ggaaggggag
    10561 caatccagca aataccgaaa ggggacgggc gaagaatatc atccatgccg acaaaatgag
    10621 cgccggaatg gcaataggca gcagatcgct tgggttaacc aatagcccca gcaccaggaa
    10681 catggcgatt tgcgccagcc aggcgaggcc gtcgaaattt tgcaggatgc cgtagcggtt
    10741 gcgaatcggc cgattaccca gcagaaaacc gcacagataa acagccagaa taccgctgcc
    10801 ttccagcgcc gtggttaaag caaaaattaa aataccgccg cttaatgcca gcaatggata
    10861 taatccggca ggcagggcga tgcgattaat catttgcagc agtaaataac caccgccaag
    10921 tccgatgaca atcccgaggc caaattgctg cagaatatcg accacgaaca tccaactgac
    10981 actgctttca tgttgctgga tcatcgcaat cagggtaatc gtcagaaaga ccgccattgg
    11041 atcattactg ccggattcaa tttccagcgt cgagccaaca cgttcgttaa gccccttacc
    11101 acccagcaga gaaaagaccg ctgcagcatc ggtagagccg acgatagcgc cgattaataa
    11161 gccttcaatc aaatcaagat taaacagcca cgccgccatc atgccggtta aaccagaggt
    11221 gataagcacg cccagcgtcg ccagcgacag tgccggtcct aacgccacac gaaaggaact
    11281 ggcctgagtg cgcatcccgc cgtcgagcaa aataatcgcc agtgccaggt tacttaccat
    11341 gtaggcgaag gggtaattat caaacgggat accgccgacg ccatcgactc ctgccagcat
    11401 gccgatcgcc agaaaaataa ccagaatagg aatgccaaga cgggaagaaa atgaactaag
    11461 taatatactg ctggtgacaa ggatggatcc taagatgaaa aggctaatta ttgttgtggc
    11521 atccaacggt cggttactcc tgattacgct gtctcttata taaaccctac catattagcg
    11581 gcagagacag cgttttactt agccctgaag cgtgattttt ttacatttta agaacaggat
    11641 gaccgctaat cgtcaactga gtgccttccc gggtgttatt cagaatggca tgtgcgccca
    11701 acggcagcgt aaccgtgcgt tgttcatgac caaaatcgag gccggtaatg agtggaatcg
    11761 acaggcggga acgcaaaaac gcgtacactg actccaggtt gtaacctgcg tcataatcat
    11821 tgggcgtgct gccgctaaag ctaccgagaa taatcgcctt ctgacgtggc aaaattcccg
    11881 catgatagag ctgcaacagc atacgttcga cgcggaaagg gtgctcgtta atatcttcca
    11941 gcaccagaat accgttctca atttttggca tccacggtgt accaatcagt gaaatcagca
    12001 tcgcaagatt gcctccccac aacgtgcctt cggtctgaca tgtcgggcct tccccttgcc
    12061 attcaatggt gaaggtttta ttgcgtaacg ccagccagaa atggtgctcg gtaaaggcgt
    12121 tcagttcatc cgcaccaaag tttgctacca gcatggggcc gctaaaggtg atgacattgc
    12181 cttgcgccag aagaccgcac tgaatggcgg taaaatcgct atgtccgcaa atgagtaacg
    12241 ggtcatgttg ttggcgagcc accagtgcct gccagtcaat atctgccagt aaacgactgg
    12301 caccgtaacc gccgcgtaca gccagcacga tggtgttggg ggtagtcagt ctggcaaggg
    12361 aattgagatc ttccaggcgc tccgtttccg tacccgcaaa acgctcacaa cgacgggcaa
    12421 taacctcgac gttattgacc tgatgcccct cgtcggtaag gcgatggata ccacgcagcg
    12481 cggcgtgctg tttaatgcag taacccgatg gggcaattaa gtgaaacaga gacatggcaa
    12541 ttccttgctg acaacagaaa cgaaatgtat atcatgccgc ttaggtgtgc cgttgtcacc
    12601 tcaacggcga ttccaggcta taaggataga agaagtgaaa ttgagatggt ttgccttttt
    12661 gattgtgtta ttagcgggtt gttcatcaaa gcatgactat acgaacccgc cgtggaacgc
    12721 gaaagttccg gtgcaacgtg cgatgcagtg gatgccaata agccagaaag ccggtgcagc
    12781 ctggggcgtc gatccacaat tgatcacggc gattatcgct atcgaatcgg gtggtaatcc
    12841 gaacgcggtg agtaaatcga atgccattgg tttgatgcag ttaaaagctt caacctccgg
    12901 acgtgatgtt tatcgccgta tgggctggag tggtgagccg acgaccagcg agctgaaaaa
    12961 tccagagcgt aatatttcaa tgggggcggc ttacctgaat attctggaaa ccggcccgct
    13021 ggcaggcatt gaagatccga aggtactgca atatgcgctg gtggtgtcat acgctaacgg
    13081 ggcaggtgcg ctgctacgga ctttctcgtc agatcggaaa aaggcgatca gcaaaatcaa
    13141 cgatttggat gctgacgagt tcctcgatca cgtagcgcga aatcatcctg cgccgcaggc
    13201 tccgcgctat atctacaaac ttgagcaggc actggacgcg atgtaaatca gtcgcgcact
    13261 ttgtccgctt tttctcgggc ttctcgctcg agagagaaaa taatccgctg taattgccgc
    13321 tccaccgtcg ggctgacgtt aagaaaacgg aagctcagac ggggagtggt gatggtttca
    13381 ttcttgccat caatcacttt gcgctcgctg atggagatta actgggcgtc aaagtgaaaa
    13441 acaccccatt gccccatgtt gacttcaatc tgagcgaagc gcatgccttc ttgtaattcg
    13501 gcaggctttg ctgtttccag taatgcgccc atgccgccta acgacaaatc atacaggcgg
    13561 aaacgtaacg tactgttatc cgccagtttg gtctggcaaa aataaggcgg atggagtggg
    13621 gcggagatgc ggaaatatcg gcgtcgttgt acaaaccata aggtgggagg cggtacggtg
    13681 ataaatgccg gaagctgcaa gtattcactc tgctgtagtt gttcaacagt aaactcgact
    13741 ttcgcaccct gagtttcggc ggtaatggta atgtgctgtg cctttagcac ggcgatgttg
    13801 tcttcggctt gactgccgaa atccagcacc agtttatccg gggttattgc cagtattttg
    13861 ctgatcagct gcccgccatt ccaactgaga cgcaaaggaa ttgcggcttt gtgcaaatcg
    13921 cgtaacacgc ccaggacggc taacggattt tgtttcagga actgctcatg gtaatgactc
    13981 acgcggaaaa actcctgact gacaaactgt ctttggttta tcggtcacag ttaacaaaac
    14041 ttaatacaaa tgtgtgaata ttttttacat gctgttctta aatcagccga catacgccca
    14101 gcatcgatcc cgcccttgcc tatacttaga gcgttgatgt aagcatttct tgcgtccgat
    14161 tcatcgaaac gagggcctga acatgggaat tattgcctgg attatttttg gcctgatagc
    14221 cggcattatc gccaagctaa tcatgccggg gcgtgatggt ggtggatttt tcctgacctg
    14281 tattctcggg atagtcggtg cggtggtcgg cggctggctg gcgaccatgt ttggcattgg
    14341 cggctccatc agtggtttta atctgcacag cttcctggtg gcggtggtgg gagctattct
    14401 cgttctgggc atattccgcc tcctgcgaag agaataagat tttcataagg cggatagcga
    14461 tacagatgcc gctatccgct ttcacatcag aacgtatact cgacacctgc tttacgggtg
    14521 gaacaaatca atatagcact tagatgcccg ataaagtcgg ttcccggtaa caacaacaga
    14581 atcctttttt aattattgtt tcgttgtttt tggtgtgatt cagaaaataa atattttaga
    14641 cattagccag tcaggcgtta aaaatggggc ggatagtgag gcggttttgg ctgcgcttat
    14701 tgaacattta gaattgatca atccttctgg cagattaagc caaaataccc gttcggcaat
    14761 gcttcagtta cgcgaagagg aatggtctga attctttttt tggttgctaa attctctgga
    14821 gtgtctggat tatgtgatta ttaatttaac tccagaaagc aaaaaaacgt tgatgagtga
    14881 acaccgtaac aatattcagg tagcaatcga tgctttatat aggcaacgtc ggtgtaaatc
    14941 accgggtgat gaaagtgaaa cacttacccg tagaaatgac gctatcttcg gcaaccacgt
    15001 atggcaaaca tttgcgcaat acttcccgcc aggacttgaa aaaccgtctg tgtagaaaac
    15061 accatagcta tggtgttatc tgccgacaac gccatccgga gaagccgaat gacgttgtcg
    15121 gagtaagcgc tggttaaggt gtgggttgtg cctctttggt tgagggttgc gtcgttgctg
    15181 acttaacggt cggacgcgtc gccggaacat tgtcacacgg ttgctctttc gggcagatca
    15241 aatccagcat tttcagcgtc acgccattgg tccagccaaa gccatcctgt aatggatatt
    15301 cgccaccgcc gccccccgtt ccggtggtgc tgacatcata tttttccacc agctttttct
    15361 cccggtcata ggtgtgctga acattggtca ggaagtgcca gctaatgtcc atcgccacct
    15421 ctttttgccc gtagttttgt aatccttctg tcgcgaccca ctgtaacggt gcccagccat
    15481 ttggcgcatc ccattgttgc ccacttttca ccgacgtggt gttcaggccg ccgggttgca
    15541 gcagatgtgt tttcgtcgcc gtcgccattt tgttggcgcg atctttcgct gccgcattga
    15601 cgtacagcgg gaacagggcg gccgcggtta actgattgcg cactttataa cttttcaggt
    15661 cgtaatcggc ataccagcct tgttgatcgt tccacatgta tttttcgatc cctttttgac
    15721 gggcatttgc cagcgtttcg tactggtttg ccatcgcgtt atctccggca gctttgctgg
    15781 cgcgggcgag gattttttcc attttaaaca tcaggctgtt cagatcgacc ggtacgatgc
    15841 tggtggtgcg taaggtattt agttgatgcg ggttgtccat ccagcgcgag ctgaaatccc
    15901 agccagacgc agcggcagag cgcaggtcgc ggtaaatttc agtggcaggt cgattcggat
    15961 tgcttttggc ggtggcaata tcttccaccc atgactctgg tcgtggcgta tcgcgatcgt
    16021 cccagtagcg gttgagaagg gtaccatcct gaagttttac aacgcgtttt tcctgttgtc
    16081 cggcttgcag gttttcaaca ccgtccatcc agtaagcata ttctttttgc atttgcggca
    16141 ggtattgctt caacgcggca tcgccttcat gctgcgccag taactctacc atcagggcaa
    16201 agaagggcgg ttgcgagcgg cttaaatagt aactgcggtt gccgttggga atatgaccgt
    16261 aagtgtctat ttcatgagca aaattggcca ccatatccgc gactttatcc cagtgaccgc
    16321 tttcggcaag tcctaacatg gtgaagtaac tgtcccagta atatacctcg cgaaaacgtc
    16381 cgcccggcac gacataaggt tccggcagcg gtaacagaga atcccatttt tcggtgtttt
    16441 cggtagaacg cgttaatacc ggccaaagtc cgtcaatatg ttcgcgcagt gactgcccct
    16501 ctggcggaac atatttctcg ccttctttcg gcagggtgaa attgacgtta acgaaatggc
    16561 gcagatcaaa tccgctctgg ttttgctgca tccgataatc agcaaggatc atcagcggat
    16621 cgctgttcgg cacggcatcg gcaaaggttt tttggtccgg aaaaagtttg gcgttttgca
    16681 catcattaaa cagcggccct aataaaatat caggcggctg tggtgttacc ggtgtttctt
    16741 ctgcctgcac cgatagcgca gcgaaacaca aaaagataca ggctggaatt aacgccattt
    16801 tttgcgggcg agaaggtgcg ggggatttca tcaatcattc tccttaggcg aaaccgaata
    16861 aaacggtatt caagtatcag aaaaccttag ttcaggatcg cactagctgc atgatcgaaa
    16921 tctcattctg cgaactgtca ggcattttag aaaagggaat taaagggata aataacggcg
    16981 aaatagagtg atcaaataac gtctgtttat tagccagcca gcgacatttg gttcgcgatt
    17041 agcgaagaaa aatcatcata aattggtgaa aaatataaca ggtagttaaa aaacattagt
    17101 gctgagtaaa ttgccggatg acatcagaac gatgccatcc gaacggtggc ttaaccctga
    17161 cggttgaaac gttgcgtttt aacgtccagc gttagctttt cttctggttg tatcgcatac
    17221 agtttctcac cctgctggca aatccagcca atctccagtt cacgggcgat tagggcgctg
    17281 tgggataccg gacttccggc gctaaggcag atacctttta caaccgccgg atccagttgc
    17341 agtactgtgg aaggataaat gttctccgcc agtagaatag ttggcgagtt aaactgcggg
    17401 agttcttctt tcgtttgggt caggtggacc agggtgcgat gcagaagatc gtccacatca
    17461 atatagcgag cttgtagata ttcatcatcc agttgctggt attgctggct aagttcttta
    17521 agaacttgct gccaggcata ttctgccgtg caatgttcgt gctgaaggag ttcgcttgcc
    17581 gccgccagca gttccggatc atctaacagt gtatggtgac cagaaaagat tgcggcaata
    17641 tcgtcaagcc cgctggcttc tgctttcgct gttaacgtca tcagatctaa taacgtgaag
    17701 tcaatggcct ggcgtaatcg atcttgttcc tcttccacgg tcagggttga tttggcctgt
    17761 accgtacata aaaccggttg ataataaaat gctttacccg aaacaggcgg aacgggacgc
    17821 agagtaggtg gagcaacttc ctccgtttca ccaaagttat cttcagccag ctgacggaaa
    17881 gcgatcagtg cctcttcagc ttctggccct ttcgcaatca ggcgcagcgt atcgttatag
    17941 cgaacttgta gtaacgcaat ctggttaata ctctccggtg tgacgcattt gccgtttttc
    18001 tccagcaaca tatcggcatt aaatgtcgat aaggtataaa ccagccggga ggctggacgt
    18061 acatgcaggc cgttacggtt ctttatgacc accgccagag aacgggcttc ttcatcgtac
    18121 gcaggacatg tgtcagagat ttcagtatcg gaggacagta aacccagttg ttcacgtttg
    18181 gcttccagcg catgcatggc gtcaaagata actttgtcga tatccgcccc cgaggccgcg
    18241 ctgaccgttg ctgccagtgt accttcgacc aacggcgcag cacacaaacg tacttttgcg
    18301 gcgatctcgg gagccagcaa ttccagcgca gtttcagcac tcaataatgc gctacccata
    18361 tccatcatga ccagcacatg gtcggcatcg gcaacagatt cgatggcctc catcactttg
    18421 acggcatcgg taccaatggg attttgtgga tcgtcaattc ccgcggcaat ggcgatttta
    18481 caactatcac tcattaacat ctgacgggct aattcaccga caccttctcc cagtcggctg
    18541 ctatgtgaaa ctatgaccag gtttaccatc accaattcct tactcttttg cggctaacgc
    18601 caacatttgc atcataaaca tcaccgaggt tgcgccggga tcctggtgac caatactgcg
    18661 ttcaccgaga taactggcgc ggcctttgcg ggcttgcatt gtaatcgtac tttgtgcagc
    18721 ggattcggcg atgctactgg ctgcttcgag cgccgccgga acagagagat tttgctcgct
    18781 ggactgacgt aacgattcca ccaccggcac ccacacatca cacatggttt tatcgccagg
    18841 ttcggctttc ccgcgactga ttacgccgtc cgcgccatcg cggaacatct ggtaaagctc
    18901 ttccagcgtc aggctttgcc gtgcctgggt cgcctgtgct gcgcgtataa agaaagtacc
    18961 gaacagcgga ccactggcac caccgacgct ggaaagcagc gtcataccgg tattcttgag
    19021 aatgaaaccg atatctttat ctgcgatagc agggagtttt tccaccactt tgctaaagcc
    19081 tcggttcata tttagcccgt ggtcagcgtc gccaatttcg cgatccagtc cggtaagata
    19141 cccgctctcg gtgttgaaaa tatcgccaca acgagtgagc cagttaacaa tttgagttct
    19201 gctcagtgac attgctttct ccttatttac cccagttaag ggccggggtg tggaccgggg
    19261 cgtcccagag agccagcgtt tcgtcatcaa ctttcagtaa ggtgattgag aaaccggtca
    19321 tatccagtga ggtgcaatac gcgccaatta aattacgctc gatagtcaat cccgcttgct
    19381 ggcaacgtgt ggtcaggcgg ttatagacgc cgtacagctc agaaagcgga gttgcgccaa
    19441 gattgttaac cagcgcaatc acccgatcgc cagactggag ctgttgtttg gtttgtggtt
    19501 cttcctgcca actgccttgt tgataatccc agaaacgcaa agtgcgatgg tatgagccat
    19561 tttccagcag ggtgtcgaac atttcatcga cggtttgatc aagggaagag aaggggcggc
    19621 ggtcaatacc cggctcacca tgaatgccga cgccaaactc catctcattg tccgccaggg
    19681 taaaagaagg tttgcccgcg gcaggaacgg tacaggcacc gagagcgata cctattgagt
    19741 ggccttgatt attcaactta cgccccagtt ccgcacaggc gtccagtgag tcgccacgct
    19801 ccgccgctgc gcctacgagt ttttcaatta atacggtgtt ggcaacgccg cgtcgcccgg
    19861 cggtataaag actgtctttt actgcaacgt cgtcatcaat gaccacagtg gtcactttta
    19921 cgccgctatc gtgcagtaac tcggttgctg tttcgaagtt aagaatatcg ccggtgtaat
    19981 ttttgataat caacagtaca ccttcgccgc catcaacttg catggcgcat tcaaagattt
    20041 tatcgggcgt cggtgaggtg aaaatttcgc ccggacaggc ccccgaaagc atcccctgac
    20101 cgatataacc acagtgcatc ggctcgtgtc cgctgccgcc acccgacagc agggcgactt
    20161 ttcctgcaac gggggcatca gctcgggtga catacaccgg ttcctgatgc agtgtcagcg
    20221 atggatgcgc tttcgccagt cctgccagtt gttcgtccag tacgtcttgc acatcattga
    20281 tcaatttttt cattattttg ctccagcaat tacggtaggg catggatgat gttcaacgac
    20341 acggcgacca ctgactgccg atgaatccat tgtgcatcag gcaagggaaa agaaaattag
    20401 cccaaattat gtttcatagt gaaacatatg ctttaacgaa tgttccatat tgaaactttt
    20461 acgtgtatta atacttaaaa ttgcgatccg gaacaccttt tgtcataagg gatgcgggat
    20521 atgagtggcg cttttaacaa cgatggtcgg ggcatatctc ccttaattgc aacctcctgg
    20581 gagcgatgca ataagctgat gaaacgggag acatggaacg taccacatca ggcccagggc
    20641 gtgacatttg cttctattta tcggcgtaag aaagcgatgc tgacgctcgg gcaggctgcg
    20701 ctggaagatg cctgggaata tatggcaccg cgagagtgtg cgctgtttat cctcgatgaa
    20761 accgcctgca ttctcagccg taatggcgat ccgcaaacct tgcagcagct aagtgcactg
    20821 ggattcaatg acggcacgta ttgcgccgag ggaattattg gtacttgtgc gctatcgtta
    20881 gcggctatct ctggtcaggc cgtgaaaacg atggccgatc aacatttcaa acaggcactc
    20941 tggaactggg ccttttgtgc aacgccgttg tttgacagca agggccgatt gacgggaaca
    21001 atagcgctgg cgtgtccggt tgagcaaact accgcagctg atttgccgtt gacgttggca
    21061 atcgcccgcg aggtcggtaa tttactgctg acggacagtt tgctcgctga aactaaccgt
    21121 catttaaatc aacttaatgc cctgttagaa agtatggatg atggcgtgat tagctgggac
    21181 gagcagggta atttgcaatt tattaatgcc caggcggcgc gggtcttgcg ccttgacgcg
    21241 acggcaagtc agggacgggc aatcactgaa ctcttaacgt tacccgccgt attgcaacaa
    21301 gcaataaaac aggcacatcc gctcaaacac gtagaagcaa cctttgaaag tcagcaccag
    21361 tttattgatg cggtgataac ccttaaaccg ataatagaaa cgcagggaac cagttttatt
    21421 ttgttgctcc atcctgtgga acagatgcgg cagttgatga ccagtcaatt aggaaaagtc
    21481 agccatacct tcgctcatat gccacaggac gatccgcaaa cccgccgctt gattcatttt
    21541 ggtcgccagg cggcgcgcag tagctttcct gtcctgcttt gtggagaaga gggcgtgggc
    21601 aaggcactgc taagtcaggc aattcataat gaaagtgagc gtgctgcagg tccttatatc
    21661 gccgtcaatt gtgagttata tggtgatgcg gcactggcgg aagaatttat tggtggcgat
    21721 cgcacggaca atgaaaatgg ccgtctgagt cggctggaac tggcgcacgg cggcacgctg
    21781 tttcttgaaa agattgaata tctggcggtg gagttacagt ctgctttgct tcaggttatc
    21841 aagcagggtg ttatcacgcg actggatgcg cggcgtttaa taccaattga tgtcaaagtg
    21901 attgccacaa cgaccgcgga cctcgcaatg ctggtggaac aaaatcgttt tagtcgccag
    21961 ctgtattacg cgctgcatgc atttgaaatt accatcccgc ctctgcgtat gcggcgtggc
    22021 agcattccgg ctctggtgaa taacaaatta cgcagtcttg aaaaacgctt ctctacgcgg
    22081 ctgaaaattg atgacgatgc cctcgctcgc ctggtttcct gtgcatggcc tggcaacgat
    22141 tttgaacttt acagcgtcat cgagaatctt gctctgagta gtgataatgg gcgtattcgc
    22201 gtcagtgatt tgccggaaca tctgtttacc gagcaggcaa cagatgatgt cagcgccaca
    22261 cgcctttcca ccagtctgtc atttgcggaa gttgaaaaag aggcaattat taacgcagcc
    22321 caggtcacag gcggtcgcat tcaggaaatg tcggctttac ttggaatcgg ccgcactacg
    22381 ctgtggcgga aaatgaagca acatggcatt gatgcagggc agtttaagcg ccgggtatga
    22441 aagacagaga cgatttctga tacatcagag tgatctatat ttcattccgg cgcacgctaa
    22501 catttttcag catcgtttaa gggcttgtct accccgtact taaaagctga agtgatatcc
    22561 tccgttgact tgcttctgat caaagaggtt accctgcgtg taatccgctt cgaggtagaa
    22621 tgtgtgctgt ttgttatact gtgcactgac tcccacgccg ttattccagc cattaccttt
    22681 gaaactgtac ttctcccggg agttgttcaa cagatattct gtatcccctg aaaactcgcg
    22741 gatagcgcca gtcttcacat acatattcag ctggctgttg cctgcggtga tgtcatatcc
    22801 cagtatcatg ctggcacgcc ccagcagcga ttcgtagtga ttcagatgta tattgagacc
    22861 attactcgcc ttcatagcca tctcattctg gtggctgtat gtgagctggg tttgcggctc
    22921 tatatagaac ccataaccag taggggtcag gttgaacctc tgcccggctt ccagggagat
    22981 gctcaatcca ttcgcagtgc cgttggcgtt aacgccgttg ttctgactgt cccgtacgtg
    23041 gaaactattt ttctggcgcg atgcttttac aacgagatcg ctgtaaaaac cgttgtgtgc
    23101 catgtaactg gcgtacattc ccatgtagtc tgaacgtgcg gtaccgtcgc caccgctata
    23161 gtccggcgat gcatgtgttg agccaatata cagaccgaca tacaacggca ttacatcaga
    23221 gagacgttta tccccaccaa actggatacc gctgtaaccc atgtcaaagc cgctcagttt
    23281 gccactggcg aaggagtcca ggcttccccc ataactgcgc agccagatat taccgtcttt
    23341 actctgattt cgcaggtcac ccattcgttg catcagcgta cggttttcaa catagtttaa
    23401 taagtaaccg acattgagat aattgccgcc agcatctgcg gtcgtggtgg gtttaggtgt
    23461 gggagtgggg gtaggcttag gatcgggttc aggcgtagga tcggggttga ctatgggagg
    23521 ctgagcggga gccggtgttg gttcaggatt aggagtcggt tccggaactg tcccggaagc
    23581 gtaaagctcc cagttagtgc catttttacg cacatcatac agatatcccc ccaactcaac
    23641 ctgagaagac gcgctgaacg aggccgcgcc gtcagtggtt tttaccactg tcagaacttc
    23701 atttcccgtt gtggcttcgc tgccctggtt acggatagcc aatacatgat taccagcact
    23761 actcccgctg atattcaata aatccccttt attattaacg ccattaccct caccaacaac
    23821 atcagcacgc ataataaagg tactgttacc gctcaggttc tctacgttta atgtggtaaa
    23881 tgtgccggca gttgatgcgt ggctggcaaa atcgacagtt gaatggctca gcgccagcgt
    23941 gtcgagatta gaattatttg ttacgttcca gacgctgtta ttcattgcaa cgtccagttt
    24001 cccgccattg acattatcgc tgagggagga acctgtccaa accgacccag agtgcatatc
    24061 cagattgatt agcccacctt tggaaagaac gctaccgttg ataagcatac gacccgaggc
    24121 gttgatgcgg ctggcggcat aaccatcgtc atgttgcgtt gcgatggcca tctggtcggg
    24181 tgtactcata tcaatgaccg gatcgctcgt gaggtcgatc tggctgttgg tcatggcata
    24241 aatccccctg gctcctgccg cgccggtgat agccaaactg tctccggtta ttctaccgcc
    24301 gctgagcgcc cacaaaccca gcgccagact gccattacga tcaaccgtaa tatcggtatt
    24361 ttgcatgttg ataacagccg ttgccgtctg ggccgaggca ccataagaac cgccggaaaa
    24421 gatgctgttt cgttgtgctg ccgtgccaga aaaattgatt gtcgcgtctg aaccactggc
    24481 tacgaggcca ccgccctgcg cggaagaaat atggctatct gcaccgattg tggttgtacc
    24541 accacgaact tcgacgccat tggccgcggc tccagttaca tcaacagtga gtgcattcgc
    24601 gctaacctgg ccaaagctcc agaggccgtg tgcattatcg ccactggttt taatggaact
    24661 gtttgttccg agatcgacaa cagagttttt ctgtacgttt atccccatgg cgctgtagcc
    24721 ctgaacatcg attgtcaggt ctgtcgccgt aaaacgcgca gcaccattgg cgttattgcc
    24781 gttgagacca ccgatataaa cacctgtact tccatcggtc ttgattttac ttccgcttcc
    24841 aagatcgaca ctggtgccat agtcattgat ggttaggcct ataccgttcg agttttcaat
    24901 ggtgaattga gtggctgtta gtgttgagct atgcccaata atgatgccgt catcgttact
    24961 cttaatggtg ctgcctgtgc ctaagtcagc atgggtatag tcaccaatta aattaatgcc
    25021 gatggcagag gtttgaccaa caacatctac tgtgagtcgg ttagctgtca gggagctgtt
    25081 tttgccctga ataatgattc cttttgcata tccagaggcg tcgttgacat tgacggtgac
    25141 atcattaccc aggttgatat tgccaggcgt gtttcctgct ggggtcatta caccatagag
    25201 gtttcctgat ggatcggcgg tatctcctgt aattctgtca ccatcattaa tagtaatatt
    25261 ggtgccatat tgacttcctg tatcagcagc tatcagcagg ggggaaaaga taattgcatt
    25321 caaaccaata gcgccaaatt ttatcagttg aattgagggc gaacggattt ttaattcagc
    25381 gagacgatcg gctttggtat tcccattgtg ttgtttgatg cccataccac tcctatatag
    25441 tacccaggct atgggtctat ggattttgtc attttgttta agtcaatgaa taatatcctc
    25501 ttatcatcat gatatttg
//
LOCUS       DBANUU010000062        25481 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000062 DBANUU010000000
VERSION     DBANUU010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.062"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            175..327
                     /gene="hokA"
                     /locus_tag="ACIE68_002500"
     CDS             175..327
                     /gene="hokA"
                     /locus_tag="ACIE68_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFX5908807.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(316..375)
                     /locus_tag="ACIE68_002501"
     CDS             complement(316..375)
                     /locus_tag="ACIE68_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908808.1"
                     /translation="MRNAVRKAGITSRRRLLLF"
     gene            complement(515..727)
                     /gene="cspA"
                     /locus_tag="ACIE68_002502"
     CDS             complement(515..727)
                     /gene="cspA"
                     /locus_tag="ACIE68_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFX5908809.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1008..1298)
                     /gene="yiaG"
                     /locus_tag="ACIE68_002503"
     CDS             complement(1008..1298)
                     /gene="yiaG"
                     /locus_tag="ACIE68_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFX5908810.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1733..2443
                     /gene="yiaF"
                     /locus_tag="ACIE68_002504"
     CDS             1733..2443
                     /gene="yiaF"
                     /locus_tag="ACIE68_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFX5908811.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2493..3467)
                     /gene="ghrB"
                     /locus_tag="ACIE68_002505"
     CDS             complement(2493..3467)
                     /gene="ghrB"
                     /locus_tag="ACIE68_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFX5908812.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3571..4230)
                     /gene="yiaD"
                     /locus_tag="ACIE68_002506"
     CDS             complement(3571..4230)
                     /gene="yiaD"
                     /locus_tag="ACIE68_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFX5908813.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4384..6717
                     /gene="bisC"
                     /locus_tag="ACIE68_002507"
     CDS             4384..6717
                     /gene="bisC"
                     /locus_tag="ACIE68_002507"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFX5908814.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6686..7126)
                     /gene="yiaC"
                     /locus_tag="ACIE68_002508"
     CDS             complement(6686..7126)
                     /gene="yiaC"
                     /locus_tag="ACIE68_002508"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFX5908815.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7123..7686)
                     /gene="tag"
                     /locus_tag="ACIE68_002509"
     CDS             complement(7123..7686)
                     /gene="tag"
                     /locus_tag="ACIE68_002509"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFX5908816.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7844..8542
                     /gene="yhjY"
                     /locus_tag="ACIE68_002510"
     CDS             7844..8542
                     /gene="yhjY"
                     /locus_tag="ACIE68_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFX5908817.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8771..9979
                     /gene="yhjX"
                     /locus_tag="ACIE68_002511"
     CDS             8771..9979
                     /gene="yhjX"
                     /locus_tag="ACIE68_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5908818.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10303..11994
                     /gene="eptB"
                     /locus_tag="ACIE68_002512"
     CDS             10303..11994
                     /gene="eptB"
                     /locus_tag="ACIE68_002512"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFX5908819.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRNWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12086..12162
                     /locus_tag="ACIE68_002513"
     tRNA            12086..12162
                     /locus_tag="ACIE68_002513"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12120..12122,aa:Pro,seq:cgg)
     gene            12197..12330
                     /locus_tag="ACIE68_002514"
     ncRNA           12197..12330
                     /ncRNA_class="other"
                     /locus_tag="ACIE68_002514"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            13076..14683
                     /gene="dppA"
                     /locus_tag="ACIE68_002515"
     CDS             13076..14683
                     /gene="dppA"
                     /locus_tag="ACIE68_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFX5908820.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14991..16010
                     /gene="dppB"
                     /locus_tag="ACIE68_002516"
     CDS             14991..16010
                     /gene="dppB"
                     /locus_tag="ACIE68_002516"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFX5908821.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16020..16922
                     /gene="dppC"
                     /locus_tag="ACIE68_002517"
     CDS             16020..16922
                     /gene="dppC"
                     /locus_tag="ACIE68_002517"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFX5908822.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16933..17916
                     /gene="dppD"
                     /locus_tag="ACIE68_002518"
     CDS             16933..17916
                     /gene="dppD"
                     /locus_tag="ACIE68_002518"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFX5908823.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17913..18917
                     /gene="dppF"
                     /locus_tag="ACIE68_002519"
     CDS             17913..18917
                     /gene="dppF"
                     /locus_tag="ACIE68_002519"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFX5908824.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSFSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18947..20218)
                     /gene="yhjV"
                     /locus_tag="ACIE68_002520"
     CDS             complement(18947..20218)
                     /gene="yhjV"
                     /locus_tag="ACIE68_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFX5908825.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20694..20801
                     /locus_tag="ACIE68_002521"
     CDS             20694..20801
                     /locus_tag="ACIE68_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFX5908826.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21177..21284
                     /locus_tag="ACIE68_002522"
     CDS             21177..21284
                     /locus_tag="ACIE68_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFX5908827.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21660..21767
                     /locus_tag="ACIE68_002523"
     CDS             21660..21767
                     /locus_tag="ACIE68_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFX5908828.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            22143..22250
                     /locus_tag="ACIE68_002524"
     CDS             22143..22250
                     /locus_tag="ACIE68_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFX5908829.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(22337..24016)
                     /gene="bcsG"
                     /locus_tag="ACIE68_002525"
     CDS             complement(22337..24016)
                     /gene="bcsG"
                     /locus_tag="ACIE68_002525"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFX5908830.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(24013..24204)
                     /gene="bcsF"
                     /locus_tag="ACIE68_002526"
     CDS             complement(24013..24204)
                     /gene="bcsF"
                     /locus_tag="ACIE68_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFX5908831.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(24201..>25481)
                     /gene="bcsE"
                     /locus_tag="ACIE68_002527"
     CDS             complement(24201..>25481)
                     /gene="bcsE"
                     /locus_tag="ACIE68_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFX5908832.1"
                     /translation="DLQCSIDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLN
                     HCSLLVINPGNNNDKQFSLLLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQ
                     LSVQQQNGIWTLVQSEEAEIQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNE
                     ARTAQAATVVFSLQQNAQIEPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLA
                     CGANMVIPWNAPLSRCLTMIESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFC
                     NAVNNMMNNPLLPAHGKGVLVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFL
                     SFCRINDLDTALNHIFPLPTGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLT
                     QSSKPVINAEHDGRHWRRIPEPMRLLDDAVERSS"
BASE COUNT         6185 a         6316 c         6752 g         6228 t
ORIGIN      
        1 aaacttatta agctagaata gccacgggtg cttgagactg tttgtctcag gtattcaccg
       61 aaaggcagac agagaaaagc cccacctgac tataaatcaa agtgaggcta ccctatgcct
      121 gaacaccata aggttagcct cttactcgtt ggaaatcaac acagggggct gggaatgccg
      181 cagaaatata gattactttc tttaatagtg atttgtttca cgcttttatt tttcacctgg
      241 atgataagag attcactgtg tgaattgcat attaaacagg ggagttatga gctggcggca
      301 tttttagcct gcaatttaaa agagtaagag tcttcggcgg gaagttattc ccgcctttct
      361 tacggcgttg cgcattctca ttgcacccaa atttattttt cacaaaaata atactagatt
      421 ttattacgcg atcgattatt tatttcctga aaaacaaata aaaaaatccc cgccaaatgg
      481 cagggatctt agattctgtg cttttaagca gagattacag gctggttacg ttaccagctg
      541 ccgggccttt agcgccgctt tcgatggtga aggacacttt ctgaccttcg tccagagatt
      601 tgtaaccatc gttctggata gcagagaagt gtacgaacac gtctttagag ccatcgtcag
      661 gagtgatgaa gccgaagcct ttgtcagcgt tgaaccattt tacgatacca gtcattttac
      721 cggacatagt gtattacctt taataattaa gtgtgccttt cggcgatatg gcgtgcttta
      781 cagattttga agcgttaaag gaatgtgcac tacgaggggt atcaacgata actcttgaag
      841 ggacttgcct tactacactg ctttaatggt ctgtacgtca aaccgttgat gtgcattaag
      901 ccacgcattg gcgggtgatg caacaattat ttttcatatt tatgattaat cggttggaaa
      961 acggtcctgt catcaggacc gtaaacagca ataaagtgga taaaagtcta ttccatcaac
     1021 tgcttactta atgccgggtt ggcttgaatc aaacgcatca attttagttc ggcacttgaa
     1081 ggcttcacgc gtctggattc ccattccttt accatggcga ctgatacgcc caaaacccgg
     1141 gcgaaatcat cgatttttaa tcctgtccct tttcgtaact gctcaatttc ggtacaggac
     1201 gttgttctgt gcgtcagggt aattttctgc gtttcatctt taaaaacaat ctgttccagg
     1261 ctgctcaaca gctcatgcat tggatcttta tattccattg aaaactcctc aaatcacact
     1321 gcgggatcgt gaattgcatc gaagctcatt aagaatagtc gggaaaagca gacacagcgg
     1381 ggtgaagtca gggcatgctc tggctatgat tcgattcagc cagtcattta gcgtgactta
     1441 tagccaccta acgatctgat aaatgccgat atatctctta ccactcctgg tttgtaaaga
     1501 tttcgaaaat ctaaccttct gcgaaggcgt aaatctctgg ctacaaaaaa agcaacggca
     1561 ctatcatttt tttaacaatg aaacaacaaa ttaaccttgt ttcattttct ccatgatttt
     1621 accaggaaga ttacgtagaa agggtatcct gcaagcctgt cctggactat ccttgtcacg
     1681 tcagacacgc gtgtgtcgtt gtgcgctttt tttgggtgaa aggagtaaga aaatggcgac
     1741 aggaaagtcc tgctctcgct ggtttgcgcc tcttgcggcg ttattaatgg tagttagcct
     1801 gagtgggtgt tttgataaag aaggcgatca gcgtaaagcg tttatcgact tcctgcagaa
     1861 tacagtgatg cgtagcggtg aacgtctacc aaccctgact gccgatcaga aaaaacagtt
     1921 tggtcctttt gtctctgatt acgcgattct gtatggttat tctcagcagg tgaatcaggc
     1981 gatggattcc ggtctgcgcc cggtcgtaga cagcgttaac gcaattcgcg taccgcagga
     2041 ttacgttacg caaagcggtc cgctgcgtga aatgaacggt tcgctgggtg tgctggcgca
     2101 acaactgcaa aatgcgaagt tgcaggccga tgctgcgcac tctgcgctaa aacagaccga
     2161 tgatttgaaa ccggtctttg atcaggcatt caccaaagtg gtaacgacgc cagctgatgc
     2221 attgcaaccg ttgattccgg ctgcacaaac tttcacgcaa caactggtga tggttggaga
     2281 ctacattgct cagcaaggta ctcaggtgag ctttgtcgca aatggcatcc agttcccgac
     2341 ttcacagcag gcaagtgaat ataacaaact gattgcgcca ttaccggcac aacatcaggc
     2401 gtttaatcag gcctggacta cagcagttac tgcaacccaa taaagagtaa aagcccgagc
     2461 gattatcggg ctttacttca cgcagtcgcg gcttagtccg cgacgtgcgg attcacacag
     2521 ttcttctcaa cctttccttg taacgcatca atcaaattat ccacggcaca ggcggccatg
     2581 ccataacgcg tctcatgggt ggcagatcca atatgcggta ctgcgacgac gttggccatt
     2641 gagagcaacg gcgaatctac ggacagtggc tcttgttcga agacatccag cccggcggcg
     2701 tgaatttcgc ctttctgcaa tgcagcaatc agtgcatttt catcaaccac cgggccacgt
     2761 ccggcattaa tgaaaatggc ggaggatttc attttggcga attgttctgc gccaaacaga
     2821 tgatgcgtct catcagttaa cggcaggatc aggcaaacga aatctgactc ttgtaacaga
     2881 gtatccaaat cgcagtagcg ggcgttgaag cgttcttctg cttctttatg gtggcggcgc
     2941 gcgttataga ggatgggcat gttgaagcca aagtgcgcac gttgtgccag cgccataccg
     3001 atccgtccca tcccgacaat gcccagtgtt ttatggtgaa cgtcagtgcc gtaccagtcc
     3061 gggcctatgc tcgcggtcca ttcgcctgct tttacccgtt ctgctacctc cacaacccga
     3121 cgagcggtag acaacaccag cgccatcagc gtatcggcga cggtttctgt taatacggtt
     3181 ggcgtgtgca tcagcagaat ttttcgggcg gtaagcgcat cgacatcaaa attgtcatag
     3241 ccgacggaga tcgttgatgt ggcacgcagt ttcggcattt tttccagcaa tgcggcatca
     3301 acattctcgt ttgaacccag taaaccttca gcttcggcaa aaattgctgc attttgttcg
     3361 acggtttgtg ggctgaggtt tgccacctgg tgaacggtga aatgctcttg caggcgttgc
     3421 agtaaatcat caggtaaggc tttgtagagg ataacggacg gcttcatgct tctctccatt
     3481 cactgataag gcgatcacct tagcctgaaa tatgatgaag gaaaactgaa gaatgccagg
     3541 tgatttctgc atcacctggc atgaaaggga ttacagcggg cttaaggtaa tttctacacg
     3601 gcggttttgc gccttacctt ctgcggtgct gttgctggcg attgggttag ccgggccaag
     3661 gccctgagta cggatgcggc tggcgtccac gccctgggtg atcaacgcgc tggcaacgga
     3721 atccgcacgt tgctgggaga gacgcatgtt caggtcgtga ccacccgtgc tgtcggtata
     3781 accaatcacg ttaaccgccg ttttcggata ctctttcagc accattgcca cgccggtcag
     3841 ggtgttagcg cccgcaggtt tcagggtcgc gctgctgctg tcgaaggtca cattgttcgg
     3901 catattgagg ataatgttat ccccgctgcg ggttacgcta acaccagtgc cgcgcatttt
     3961 gtcacgcagc ttcgcttcct gcacatccat gtaataaccc acgccgccgc ccagagctgc
     4021 gcctgctgct gcgccaatca gcgcgccttt accgcgatct ttcttcgaag aagagagcgc
     4081 accaataccc gcgcccacga gagagcccag acctgcgccg atagcagatt tacctgcttc
     4141 gcgttcgccg gtgtaagggt tagttgtgca gccagatacc gccagagcgc cactcactac
     4201 ggcggcaata agataaacac gtttcttcat tgttaatcct taataacctt ttttattctt
     4261 tgccacgggt tccgtggcgg gagattatgc cgcgtgaaca tgaagattat tcctgggaat
     4321 actcggaaat ttgtaagtaa tatttaactg ctcaatacat ctaacctttc aggagtcttc
     4381 ggtttggcca actcatcctc acgatattcc gttctgactg ccgcccactg ggggcccatg
     4441 ctggttgaaa ccgacggcga aaccgtgttt agctcgcgtg gcgcgttagc cacaggaatg
     4501 gaaaactcct tgcagagcgc ggttcgcgac caggttcaca gcaatacgcg ggtacgattt
     4561 ccaatggtgc gcaaaggctt tctcgcgtca ccggaaaacc cgcaaggcat tcgtgggcag
     4621 gatgaatttg ttcgcgtgag ttgggatgag gcgctggatc ttattcacca acaacataaa
     4681 cgcattcgtg aggcttatgg tccggcatcg atttttgctg gttcctacgg ctggcgttca
     4741 aacggcgtgc tgcataaggc ctcgacatta ttacaacgct atatggcgct ggcaggcggt
     4801 tataccgggc atctggggga ttattcgact ggtgcggcac aggcgatcat gccgtatgtc
     4861 gtaggtggca gcgaagttta tcaacagcaa accagttggc cgctggtgct ggaacatagc
     4921 gatgtcgtgg tgctgtggag tgctaatcca ctcaatacgc tgaaaattgc gtggaatgca
     4981 tccgatgagc aggggctttc ttacttttct gcactgcgtg acagtgggaa aaagctgatc
     5041 tgcattgatc caatgcgatc ggaaaccgtc gatttctttg gcgataaaat ggagtgggtg
     5101 gcaccgcaca tgggcaccga tgttgcgctg atgctgggaa tcgcccatac gctggtggaa
     5161 aatggttggc acgacgaagc gtttctggcg cgttgcacca caggttatgc cgtcttcgcc
     5221 tcttatttgc tgggcgagag tgacggaata gcaaaaaccg ccgaatgggc agcagagatt
     5281 tgtggtgttg gcgcagcgaa aatccgcgag ctggcggcta ttttccacca aaataccacc
     5341 atgctgatgg caggctgggg aatgcagcgc caacagtttg gtgagcaaaa acactggatg
     5401 atcgtcacgc tggcagcaat gttggggcaa atcggcacac ccggcggcgg ttttggtctt
     5461 tcttaccatt ttgccaatgg tggtaacccc acgcggcgtt ctgcggtgct ctcttccatg
     5521 cagggcagct tgccgggtgg ctgcgatgcg gtggataaaa tccctgttgc ccgcattgtt
     5581 gaagcactgg aaaaccctgg tggcgcatat caacacaacg gtatgaaccg acatttcccg
     5641 gatattcgtt ttatctggtg ggcgggcggt gccaacttta ctcatcatca ggataccaat
     5701 cgtctgatcc gtgcctggca aaaaccggag ctggtggtga tctctgaatg cttctggacg
     5761 gcggcggcga aacacgcgga tatcgttctg cctgcgacta cctcttttga gcgtaatgat
     5821 ctcaccatga ccggtgatta cagtaatcag catctggtgc cgatgaagca agtggtgccg
     5881 ccacgctatg aagcgcgtaa tgattttgat gtttttgccg agttgagtga acgttgggag
     5941 aagggcggct atgcgcggtt tacggaagga aaaagtcagc tgcaatggct ggaaacgttt
     6001 tataacgttg cccggcagcg cggggcaagc cagcaggttg aattgccgcc atttgctgag
     6061 ttctggcaag ccaaccagtt aattgagatg ccggaaaacc cggacagcga gcggtttatt
     6121 cgcttcgctg atttttgccg cgatccgctg gcgcatccgt taaaaaccgc cagcggcaag
     6181 attgaaatct tctcacagcg tattgccgat tacggttacc cggattgccc tgggcatcca
     6241 atgtggctgg agccggacga atggcagggc aatgccgaac cagaacagtt gcaggtactt
     6301 tctgcccatc cggcgcaccg cctgcacagc cagctgaatt acagttctct gcgcgaattg
     6361 tacgcggtag caaaccgtga gcctgtcacc attcatcctg acgatgctca ggcgcgcggc
     6421 ataaccgaag gggatatggt gcgggtgtgg aactcccgtg ggcagatcct tgccggagcg
     6481 gtcattagcg agggaattaa acctggcgtg atttgcattc acgaaggggc atggccggat
     6541 ctggatttaa ccgctgacgg tatttgtaaa aacggcgcag tgaacgtgct gaccaaagat
     6601 ctccccagct cgcggctggg gaatggctgt gcgggtaata cggcgctggc atggctggaa
     6661 aaatacaacg gtccggaact gacacttaca gcgtttgaac caccggccag ctcataatcc
     6721 aggtgggtag ttgggtttca tcctgccatg cgcaatcgac aatgtgaaaa ccctgcgcac
     6781 ggtaaaaatc tatcgccggt tgattttttt gataaacctc cagcatcaga tggggatagc
     6841 gctgctgcac atactgcatc agcgccttac caataccgcg tctgacggcc ttcggtgcga
     6901 caaacattgc tgccagaaat cggccttcca taatgctgac aaaaccgaga agcttaccgt
     6961 cctcttccca gacccagttt tgcgcgttgg caagataggc atcccgcacc agcggaatgc
     7021 agtcacgcca gtaattcgct tttataaagg gatgccccca ggttgtactt tccagccaca
     7081 gttcgaggat cgcggggagt tctgaacgtt gcgcttcccg aatcatggtt tatttcccgg
     7141 atagcaacag cagccaacca catgatcatt caccagccca catgcctgca taaaggagta
     7201 acagattgtg gtgccgacaa acttaaaacc acgttttttc agtgccttag atagggcgtc
     7261 ggaggcggac gtagatgtgg gaatttcgct caacgttgtg gcttgtgtca cctgtggctg
     7321 atgatttaca aacgaccaga caaagtcggc aaacggttcg ccgttctgtt ccatttgcag
     7381 gtacgcccgc gcattaccaa taattgcctg aattttccct cgatggcgga taatcccggc
     7441 gtcctgtacc agtctttcga catcctcttc ctgcattgct gcgaccttca ccggatcgaa
     7501 ctgatgaaag caggcgcgat agttttcgcg ttttttgagg acggtgatcc acgataaccc
     7561 agcctgctgc ccttcaaggc agatcatttc gaacagtttt ttactgtcag tttcaggcac
     7621 gccccattca ttatcatggt aggcaatata aagcgggtcc tgactcaccc agccgcaacg
     7681 ttccatactt tccctcgcta tgtacccgat cgtaaaaatt tcagccgctt ttcttgacgc
     7741 atcggtgaaa aagtcaatat ttaatacagg gctactatgc ccgatatcct tcatgcaatg
     7801 acaataatat tgccgaattc ggctcttccc tggagtcgtg ttaatgatca taaaaaaaag
     7861 cggtggtcgc tggcagctaa gcctgctggc gagcgtggta atcagtgcct tttttctcaa
     7921 cacagcttac gcctggcaac aagaatatat cgttgatacg caacccggac attccacaga
     7981 gcgttacact tgggatagtg atcatcaacc tgattacaac gatattttgt cgcaacgtat
     8041 tcaaagtagc caaagggcgc tgggactgga agtcaatctg gcggaagaaa ctcctgtgga
     8101 tgtgaccagc agtatgagta tgggctggaa ttttccttta tatgaacagg ttacaaccgg
     8161 cccggtcgcg gcattacatt acgatggcac aaccacctcg atgtataacg agtttggcga
     8221 cagtactacc acgctgaccg atccgttatg gcatgccagc gtgagtagct taggctggcg
     8281 tgttgactcc cggcttggcg atctccgacc ctgggcgcaa atcagctata accagcaatt
     8341 tggcgagaat atctggaagg cgcaatcagg cctgagccgg atgacggcga caaaccagaa
     8401 cggcaactgg ctggatgtca ccgtaggcgc tgatatgttg ctcaatcaaa atattgccgc
     8461 ctatgccgcg ctaactcagg cagaaaatac cactaataat agcgactatc tgtatacgat
     8521 gggggttagc gccagatttt aacgtaacag tcacaattga aaccattgaa taacaacagt
     8581 tgtgtcgata gtgggtgcta acttaccaaa taataaattt ggtgaataat tgtcgcgtca
     8641 ttcattcctg aactaaggca tttcattccg ttctgatggc atttcatgcc gtttttcccc
     8701 aggcataaaa tgcacttcgt tatggttgtc ggcagagatt tttccttttt attactgcag
     8761 gaatactgcc atgacacctt caaattatca gcgtacccgc tggctgacac tcatcggtac
     8821 tatcattacc cagtttgcac tggggtcggt ttatacctgg agcctgttta atggcgcgct
     8881 ttctgccaag ctggatgcgc cggtaagcca ggtcgctttc tctttcggct tgttaagtct
     8941 ggggctggca atttcgtctt ctgttgcggg caaattgcag gaacgttttg gcgttaaacg
     9001 cgtcaccatg gcttccggca ttttgctggg attaggcttc ttcctgaccg cgcattccaa
     9061 caacctgatg atgctgtggt taagcgccgg tgtgctggtc ggtctggcgg atggcgcggg
     9121 ttacctgctg acgctctcta actgcgtgaa gtggttcccg gagcgtaaag gtctgatatc
     9181 cgcgttcgct atcggttctt atggtctggg tagcctgggt ttcaaattta tcgacacgca
     9241 gctgctggaa acggtgggtc tggaaaaaac ctttgtgatt tggggagcga ttgcgctggt
     9301 gatgattgtt ttcggcgcaa cgttaatgaa agacgcacca aaacaggaag tgaaaaccag
     9361 caatggtgtg gtggagaaag attacacgct ggcagagtcg atgcgtaaac cgcagtactg
     9421 gatgttagcg gtaatgttcc tgactgcctg catgagcggc ctgtacgtga ttggggtagc
     9481 gaaagatatc gcccaaagtc tggcacacct tgatgtggtt tccgcagcca atgcagtaac
     9541 ggttatttcc atcgctaacc tttcaggtcg tctggtgctg ggcattctgt ctgacaaaat
     9601 tgcccgtatc cgtgttatca ccattggtca ggtgatttcg ctggtgggta tggcggccct
     9661 gctgtttgca ccattaaatg cagtgacgtt ctttgcagcg attgcctgcg tggcatttaa
     9721 ctttggcggc actattaccg tctttccatc actggtcagt gagttcttcg gcctcaataa
     9781 cctggcgaaa aactacggtg tgatttacct cggtttcggt atcggtagca tttttggttc
     9841 gattatcgcc tcactgtttg gcggtttcta tgtgaccttc tacgtgattt tcgccctgct
     9901 gattctgtca ttggcgcttt ctacgacgat tcgtcagcca gagcagaaaa tgttgcgtga
     9961 ggcgcatggc tccctttaat gctcagccgc gagctactgg gcttcagctt ccgtgtgcat
    10021 caaacgcatc aacgaaatgt ataaacaatg actcgtaaaa tttgggccag ttaactggcc
    10081 ctttttactg ctttttgtaa cgctaaagtc tcttttcaaa cttgcatttt tgtaaatttg
    10141 tgcttcatgc acactctttc cccacacttt ttcgctttgc tgtggtctac ttattcgcgc
    10201 gtgtagattt tacttatctg actacctccg cacttttccc ctgccgggcc tgaaaagccg
    10261 ctaagcaggg tgttatcacc tgtttgtcca gggtttgttt gcatgagata catcaaatcg
    10321 attacacagc agaagctgag ctttttgctt gcaatctata ttggcctttt tatgaatggc
    10381 gcggtttttt atcgccgctt cggcagctat gcgcacgatt ttaccgtctg gaaaggcgtt
    10441 tctgctgttg ttgaactggc cgccaccgta ctggtgacct tctttttact acgtcttcta
    10501 tcgctgtttg gccgccgcaa ctggcgtatt ctggcgtcgc tggtggtgct cttttccgca
    10561 ggtgccagct attacatgac cttccttaat gtggtcattg gttatggcat catcgcttcc
    10621 gtcatgacca ccgatatcga cctgtcaaaa gaagttgttg gtctgaactt tattctctgg
    10681 ttaatcgccg ttagtgcatt gcctcttatc cttatctgga ataaccgctg tcgctacacc
    10741 ttgctccgac aactgcgaac cccagggcag cgtattcgca gcctggcggt cgtcgtactg
    10801 gcgggtatta tggtttgggc accgattcgt ttgctggata tccagcagaa gaaagtggag
    10861 agggcgaccg gcgttgattt gccgagttat ggcggtgtcg tagcgaactc ttatctgcca
    10921 tcaaactggc tttctgcgtt ggggctgtat gcctgggcgc gggtcgatga atcttccgat
    10981 aataattcat tgcttaatcc ggcgaagaaa ttcacttatc aggcaccgca aaacgttgat
    11041 gacacttatg tcgtgtttat catcggtgaa accacgcgtt gggaccatat gggcattttc
    11101 ggctatgagc gtaataccac gccgaaactg gcccaggaga aaaatctggc ggcgttccgt
    11161 ggttactcct gtgataccgc aaccaaactc tcactgcgtt gcatgtttgt acgtcagggg
    11221 ggcgcggaag ataatccgca gcgcacatta aaagaacaga acattttcgc ggttctgaag
    11281 cagttaggat tcagttctga cctctacgct atgcagagcg aaatgtggtt ctacagcaac
    11341 acgatggcgg acaacattgc ttatcgtgag cagattggtg cggagccacg taatcgtggc
    11401 aagccggtag atgatatgtt gctggtagac gaaatgcagc aatcgctagg gcgcaacccg
    11461 gatggtaagc atctgatcat tctgcatacc aaaggttcgc attttaacta cacccagcgt
    11521 tatccgcgta gcttcgcgca gtggaagccg gaatgtattg gtgttgatag cggctgtacc
    11581 aaagcgcaga tgatcaactc ctatgacaac tcggtgacct atgtggatca ctttatctcc
    11641 agcgtgattg atcaggttcg cgataagaaa gcgattgtgt tctacgcagc tgaccacggt
    11701 gagtcaatta atgaacgcga gcacctgcac ggcacgccgc gtgaactggc accgccggag
    11761 cagttccgcg taccgatgat ggtctggatg tcagataaat atctggaaaa tccggccaat
    11821 gctcaggcat ttgcgcagct gaaaaaagaa gccgacatga aagtgccacg ccgtcatgta
    11881 gagctgtacg acaccatcat gggttgtctt ggctatactt caccggatgg tggaattaac
    11941 gaaaacaaca actggtgtca catcccgcag acaaaagtgg cagcggctaa ctaagcggcc
    12001 tgctgacttt ctcgccgatc aaaaggcatt ttgctattaa gggattgacg agggcgtatc
    12061 tgcgcagtaa gatgcgcccc gcattcggtg attggcgcag cctggtagcg cacttcgttc
    12121 gggacgaagg ggtcggaggt tcgaatcctc tatcaccgac caaattcgaa aagcctgctc
    12181 agcgagcagg cttttttgca tttgaagccc atgaggatga gaacctccgg gggcggggag
    12241 gttcgactcg agcgaagcga gagaacgttg cgccagcaac ggcccgcagg gcgagccacg
    12301 aagtggcgag taatcctcta tcaccgacca aattcgaaaa gcctgctcag cgatcaggct
    12361 ttttttgcat ttgtagccca tgaggatgag aacctccggg ggcagggagg ttcgactcga
    12421 cgtaggcctg ataagacgcg ccagcgtcgc atcaggcaac ggcaccgcac ttattgtcgg
    12481 atgcggcgcg accgccttat tcgaccaaca caccacacat tttcctgcca actttgcgcc
    12541 ccatatctca ctatccatta acggatttgt gacagaaacc attgtctttt tttatatttg
    12601 cttaaatctt tttttgcgtt acttatggct ggcgctggca ttttccgctg tgcgttttag
    12661 cgttcatggt attaatcgct cagatatcca ccttttcctc agattttttt cattaaatgc
    12721 ataaaactta atcaccaaat gttgctaaat attaagctga ttgttctggt catgttggag
    12781 tagcacaaat ttctctgctg caaatagctg tttgaagttt ttttaatctt tgtttgtgga
    12841 ttctcaccgt tggtgtaaat cccggttggc tgtattgacg ttttcacatt ctgttgacag
    12901 attgtaggtc acgaggggca ttttatggag gatccgcact gttacactga tgttaattag
    12961 tacggcatcc ccacctcata acgttgaccc gaccgggcaa aaaacaaaaa aggtcaggca
    13021 gcgacaaccc actgcaaagg gttaaaacaa caaacatcac aattggagca gaataatgcg
    13081 tatttccttg aaaaagtcag ggatgctgaa gcttggtctc agcctggtgg ctatgaccgt
    13141 cgcagcaagt gttcaggcta aaactctggt ttattgctca gaaggatctc cggaagggtt
    13201 taacccgcag ctgtttacct ccggcaccac ctatgacgcc tcttccgtac cgctttataa
    13261 ccgcctggtt gaatttaaaa tcggcaccac tgaagtgatc ccgggcctcg ctgaaaagtg
    13321 ggaagtcagc gaagacggta aaacctatac cttccatctg cgtaaaggtg tgaagtggca
    13381 tgacaataaa gaattcaaac cgacgcgcga actgaacgcc gatgacgtgg tgttctcgtt
    13441 cgatcgtcag aaaaacgcgc aaaacccgta ccataaagtt tctggcggta gctacgaata
    13501 cttcgaaggc atgggcttgc cggagctgat cagcgaagtg aaaaaggtgg acgacaacac
    13561 agttcagttc gtgctgactc gcccggaagc gccgttcctc gctgatctgg caatggactt
    13621 cgcctctatt ctgtcaaaag aatatgctga tgcgatgatg aaagccggta caccggaaaa
    13681 actggacctc aacccaatcg gaaccggtcc attccagtta cagcagtacc aaaaagattc
    13741 ccgtatccgc tacaaagcgt ttgatggcta ctggggcacc aaaccgcaga tcgatacgct
    13801 ggtcttctct attacccctg atgcttccgt gcgttacgcg aaattgcaga agaatgaatg
    13861 ccaggtgatg ccgtacccga acccggcaga tatcgctcgc atgaagcagg ataaatccat
    13921 caacctgatg gaaatgccgg ggctgaacgt cggctacctc tcgtataacg tgcagaaaaa
    13981 accgctggat gacgtgaaag ttcgccaggc actgacctac gcggtgaaca aagacgcgat
    14041 catcaaagcg gtttatcagg gcgcgggtgt atcagcgaaa aacctgatcc cgccaaccat
    14101 gtggggctat aacgacgacg ttcaggatta cacctacgat cctgaaaaag cgaaagcctt
    14161 gctgaaagaa gcgggtctgg aaaaaggttt ctccatcgac ctgtgggcga tgccggtaca
    14221 acgtccgtat aacccgaacg ctcgccgcat ggcggagatg attcaggcag actgggcgaa
    14281 agtgggcgtg caggccaaaa tcgtcaccta cgaatggggt gagtacctca agcgtgcgaa
    14341 agatggcgaa catcagacgg taatgatggg ctggactggc gataacgggg acccggataa
    14401 cttcttcgcc accctgttca gctgcgccgc ctctgaacaa ggttctaact actcaaaatg
    14461 gtgctacaaa ccgtttgaag atctgattca accggcgcgt gctaccgacg accataacaa
    14521 acgcgttgaa ctgtacaagc aagcgcaggt ggtgatgcac gatcaggctc cggcactgat
    14581 catcgctcac tccaccgtgt ttgaaccggt acgcaaagaa gtcaaaggct atgtggttga
    14641 tccattaggc aaacatcact tcgaaaacgt ctctatcgaa taattaaaag ccatacaaga
    14701 ctgatggcaa aggcaaaact gcctgatgtg ctccgcttat caggcctacg aaaattctgc
    14761 aatgtattga atttgcacga ttttgtaggc cggataaggc gttaacgccg catccggcat
    14821 aaacaaagcg cactttgtca acaatctgta tacccggtgg cgctgtgcct ctgcgtgcag
    14881 cgccatccgg cagcaatact tcattctctg tccgacaagg gcgggaattg atttgtgagc
    14941 aatacagaca cgcagttcca ggctgcgggt cactatagag aatccgggtt atgttgcagt
    15001 ttattctccg acgtttggga ctcgtcatcc ccacgtttat cggtattacc cttctcacat
    15061 ttgcctttgt ccacatgatc ccgggcgatc cggtgatgat catggcaggc gaacgtggga
    15121 tctcccctga gcgtcacgcg cagctgctgg ctgaactcgg cttagataaa ccgatgtggc
    15181 agcagtatct ccattacatt tggggcgtaa tgcacggcga tctaggcatt tcaatgaaaa
    15241 gccgaattcc agtatgggaa gagttcgtgc cgcgctttca ggccacgctg gaacttggcg
    15301 tctgcgcgat gatttttgct acggcagtcg gtattccggt tggtgtgctg gctgcggtta
    15361 aacgcggttc cattttcgat cacacagcgg ttggcctggc gctgaccggc tactcgatgc
    15421 cgatcttctg gtggggcatg atgctgatca tgctggtttc ggtgcactgg aacctgacgc
    15481 ccgtctccgg tcgcgtgagc gatatggtgt tcctcgatga ctccaatccg ttaaccggtt
    15541 ttatgctaat cgacaccgcc atctggggtg aagacggcaa ctttatcgat gccgtcgccc
    15601 atatgatctt gcctgccatt gtgctgggta ctattccgct ggcggtcatt gtgcgtatga
    15661 cacgctcctc gatgctggaa gtgctgggcg aggattacat ccgcaccgcg cgcgccaaag
    15721 ggctaacccg catgcgggtg attatcgtcc atgcgctgcg taacgcgatg ctgccggtgg
    15781 tgaccgttat cggcctgcag gtgggaacat tgctggcggg ggcgattctg accgaaacca
    15841 tcttctcgtg gcccggtctg ggacgctggt tgattgacgc actgcaacgc cgcgactatc
    15901 cggtagtgca gggcggcgta ttgctggtgg cgacgatgat tatcctcgtc aacttgctgg
    15961 tcgatctgct gtacggcgtg gtgaacccgc gtattcgtca taagaagtaa ggggacatca
    16021 tgtcacaggt tactgaaaat aaagtgatta gcgcaccggt gccgatgacc ccgttacagg
    16081 agttctggca ctattttaaa cgcaacaaag gcgcggtcgt cgggctggtt tacgtcgtca
    16141 tcgtgctgtt catcgcgatc tttgccaact ggattgcacc ctataacccg gcggaacagt
    16201 tccgcgatgc actgctcgcc ccgccagcct ggcaggaagg cggcagcatg gcgcacttgc
    16261 tgggcaccga tgacgtaggc cgtgatgtgc tgtcgcgcct gatgtacggt gcgcgcctgt
    16321 cgctgctggt tggctgtctg gtagttgtgt tatcgctgat tatgggcgtt attctcggcc
    16381 tgatcgccgg ttactttggc ggcctggtcg ataacatcat tatgcgcgtg gtcgatatca
    16441 tgctggcgct gccaagtctg ctgctggcgc tggtgctggt ggcaattttc ggcccgtcga
    16501 ttggtaacgc cgcgctggca ctgaccttcg ttgccttgcc gcactatgtg cgcttaaccc
    16561 gcgccgccgt gctggtggaa gttaaccgcg attacgtcac cgcgtctcgc gtggcgggtg
    16621 ccggggcgat gcgtcagatg tttattaaca tcttcccgaa ctgccttgcg ccgctgattg
    16681 ttcaggcgtc gctcggtttc tctaacgcca ttctcgatat ggctgctctt ggtttcctcg
    16741 gcatgggggc acagccgcca acgcctgagt ggggcaccat gctctccgac gtgttgcagt
    16801 tcgcgcaaag cgcctggtgg gtcgtgacct tcccgggtct ggcgatcctg ctgacggtgc
    16861 tggcatttaa cctgatgggt gacggtctgc gtgacgcgct cgatcccaaa ctgaagcagt
    16921 aagaggttcg agatggcgtt attaaatgta gataaattat cggtgcattt cggcgacgaa
    16981 agcgcgccgt tccgcgccgt agaccgcatc agctacagcg taaaacaggg cgaagtggtc
    17041 gggattgtgg gtgagtccgg ctccggtaag tcggtcagtt cactggcgat tatggggctg
    17101 attgattatc cgggccgcgt aatggcagaa aaactggagt ttaacggcca ggatttgcag
    17161 cgtatctcag aaaaagagcg ccgcaacctg gtgggtgccg aagtggcgat gatcttccag
    17221 gacccgatga ccagccttaa cccgtgctac accgtgggtt tccagatcat ggaagcgatt
    17281 aaggtgcatc agggcggtaa taagagcact cgccgtcagc gggcgattga cctgctgaat
    17341 caggtcggta ttcccgatcc ggcatcgcgt ctggatgttt acccgcatca gctttccggc
    17401 ggcatgagcc agcgcgtgat gatcgccatg gcgattgcct gtcggccaaa actgctgatt
    17461 gccgatgaac cgaccaccgc gctggacgtg accattcagg cgcaaatcat cgaactactg
    17521 ctggagctac agcagaaaga gaacatggcg ctggtgttaa ttacccatga cctggcgctg
    17581 gtggcggaag cggcacataa aatcatcgtg atgtatgcag gccaggtggt ggaaaccggt
    17641 gatgcgcacg ccatcttcca tgcgccgcgt cacccgtata ctcaggcatt gctgcgtgcg
    17701 ctgccagaat ttgctcagga caaagaacgt ctggcgtcgt tgccaggtgt cgttcccggt
    17761 aagtacgacc gcccgaacgg ctgcctgctt aacccgcgct gcccctatgc cactgacaga
    17821 tgtcgcgctg aagaaccggc gctgaatatg ctcgctgatg ggcgtcagtc caaatgccat
    17881 tacccacttg atgatgccgg gaggccgaca ctatgagtac gcaagaggcc acctcgcaac
    17941 aaccgctgtt gcaggctatc gacctgaaaa aacattatcc ggtgaagaaa ggcatgttcg
    18001 cgccggaacg tctggtgaag gcgctggacg gcgtttcgtt taaccttgaa cgtggcaaaa
    18061 cgctggcagt agtgggcgaa tctggctgcg gtaaatcgac cctcggtcgg ttgctgacga
    18121 tgatcgaaac gcccaccggt ggtgagctgt attaccaggg gcaggatctg ctcaagcacg
    18181 atccgcaggc gcagaagctg cgtcggcaga aaatccagat tgtcttccag aacccttacg
    18241 gttcgctgaa tccgcgtaaa aaagttgggc aaattctcga agagccgctg cttattaaca
    18301 ccagcttcag caaagaacag cgtcgggaaa aagccctgtc gatgatggcg aaagtcggcc
    18361 tgaaaaccga gcactatgac cgctatccgc atatgttctc cggcggacaa cgtcagcgta
    18421 tcgctattgc ccgtggtctg atgctcgacc cggacgtggt gattgccgat gaaccggttt
    18481 ctgcgctgga cgtctcagtg cgtgcgcagg tgctgaatct gatgatggat ttgcagcagg
    18541 agttggggct ttcttatgtc tttatctccc acgacctgtc ggttgtggag cacattgctg
    18601 atgaagtgat ggtgatgtac ctgggccgct gcgtggagaa aggaacgaag gaccaaatct
    18661 tcaataaccc gcgccatccg tacacccagg cgctgctctc cgcgacgcca cgcctgaacc
    18721 cggacgatcg tcgtgaacgc atcaaactca ccggtgaact gccaagcccg ctgaatcctc
    18781 cgccgggttg cgccttcaac gcccgctgtc gtcggcgctt cggcccctgc acccagttgc
    18841 agccgcagct aaaagactac ggcggtcaac tggtagcttg ttttgctgtt gatcaggatg
    18901 aaaatccgca gcgttaactc acaaaaccgg ggccatgcgc tccggtttac tcaatcagct
    18961 tcaacagtgg cgacaagcat agcaaaatgc cgtaaagcag aattagccag gctttaaatc
    19021 cgcgaagttt ttccagttgt gcgactttat agatcaggaa aaacggaatg aggcacgaca
    19081 caatgccata taacgggctt cccaactgaa agaacaccag caccgataca cgaaacgaaa
    19141 cccaaatcgt caatgtaatg acgataaaag cgcagatcgc cagagtcagc atgcgtgagt
    19201 taattttctt ggtatcaata attcggctta acagattgag aataatgcct ttaatagcct
    19261 cgtggaaacc gagataaatg ccaaagaatg cggtcagtac ggcaaagata ttcagcaccg
    19321 tagaggtgat atgaatgata tgtccaggga tcacctgcgc ggccagcgcc agtgccgaga
    19381 tattttgttc aaaggcagaa acggcttctt cgtggctaat tgagaaggta aacgaaaagg
    19441 caaaaaacag gatcaccgcg atgagcgtga tataactaat tcggtgggta cgcagcgcga
    19501 gccgggttgc cagtacttta tctgcttccc gtttacgata ggcaatattc attgggttaa
    19561 gtacctgaat aaataccgca gaaaagaagc aaaatggaat tgtaagcagg acatcgcgga
    19621 aaaagacgga ggcttgcggg aaggcggtta tattggcgaa attccagtgc gggatcatcg
    19681 caaaaccgaa cacgacaata atccctactt tgaccaccac cattggcccg gaaatcttaa
    19741 acagtaatcg ttcaccacca gacgcaatcg ccaccagtac ggcgaaaata gcgactttat
    19801 aaagtagaga ttgtgaaaga tcggcatcgg ttaaaccgaa ggttttcagg tacgaggcgc
    19861 tgtcgaaaac cacggagaga gagtagataa aaatcccgtg gataatcatc aaaaagtaga
    19921 taaccccgag gaaaattccc cagttcttcc ccaggtaatg actgataata tcggtgtagt
    19981 cattacagga atcgctttca gaaagggttt ttaaataaat gtcctgcact acccaggtgg
    20041 caggataagc aatgatcgct gcggtaataa atacccaaat tcccttcaag ccaatttgta
    20101 ctggcatcag cacggttccg gcaccaatcg ccatgcctat gcataataaa acccagccga
    20161 agtcgtagcg tgtaaacggc aatttctgat tgtgtttcga tagtgtgttg tgctgcataa
    20221 agtcctgtcc agaaagaagt cggcctcgca aaaatgaata aacccagttt ctcttgcgca
    20281 acaggtggag aagtcgaata cgcagtatgt aaaaaatgag gcaacagatt cactggggag
    20341 gtcgcgagga ggcgcaattc tacaaaattg cgcatttcac taccagtctg gagtcagccc
    20401 ggatatgaac tgcaactatt gcaaataaag aaattaagct ggaaggcatc gcgcaatgag
    20461 gaagattgcc gggtgctgac agtaaaaagc aatcgtaaaa tcagcggcaa gccggaaggg
    20521 ttccggtgag gcgcaatgtt gcgggggctt tatccctggt ggcattggtt gctggagaga
    20581 gaaaaccccc gcacgttgca ggtatatacc tgacaacata acgggggcta atcttgaacc
    20641 tagaccactc aagaatagcc gcgaaacgtt gtcattacaa cacaggcggc tatatgacgc
    20701 tcgcagagct gggcatggcc ttctggcatg atttagcggc tccggtcatt gctggcattc
    20761 ttgccagtat gatcgtgaac tggctgaaca agcggaagta gcgtgtcatg tgggcgtcag
    20821 gctgccgtaa tggcaatttg cgcccggacc aggccgcagg agtgaaactc tgcggccttt
    20881 ttcgtaagaa aaatgcaacg gttgcaagcg gataatattc tgcggaatgc gcagcacaga
    20941 gtgaagatga gacggattcc ggaacagaaa aacagtcgta gaatcagcgt caagccggaa
    21001 ggattccggt gaggcgcaat gttgcggggg cttaatccct ggtggcattg gttgctggag
    21061 agagaaaacc cccgcacgtt gcaggtatgc acctgacaac accacggggg ccattcttgc
    21121 atccaaacaa tacaagaata gccgcgaaac gttgtcatta caatacaggc ggctatatga
    21181 cgctcgcaga actgggcatg gccttctggc atgatttagc ggctccggtc attgctggta
    21241 ttcttgccag tatgatcgtg aactggctga acaagcggaa gtaacgtgcc atgcgggcgt
    21301 caggctgccg taatggcaat ttgcgcccgg atcaggccgc aggggtgaaa ctctgcggcc
    21361 tttttcgtaa gaaaaatgca acggttgcaa gcggataata ttctgcggaa tgcgcagcac
    21421 agagtgagta agtgatggat tccggaacag aaaaacagtc gtagaatcag cgtcaagccg
    21481 gaagggttcc ggtgaggcgc aatgttgcgg gggctttatc cctggtggca ttggttgctg
    21541 gagagagaaa acccccgcac gttgcaggta tgcacctgac aacatcacgg gggccattct
    21601 tgaacctgaa caactcaaga atagccgcga aacgttgtca ttacaacaca ggcggctata
    21661 tgacgctcgc agaactgggc atggccttct ggcatgattt agcggctccg gtcattgctg
    21721 gcattcttgc cagtatgatc gtgaactggc tgaacaagcg gaagtaacgt gtcatgcggg
    21781 cgtcaggctg ccgtaatggc aatttgcgcc cggaccaggc cgcaggggtg aaactctgcg
    21841 gcctttttcg taagaaaaat gcaacggttg caagcggata atattctgcg gaatgcgcag
    21901 cacagagtga gtaagtgatg gatttcggaa cagaaaaaca gtcgtagaat cagcggcaag
    21961 ccggaaaggt tccggtaagg cgcaatgttg cgggggcttt atccctggtg gcattggttg
    22021 ctggagagag aaaacccccg cacgttgcag gtatgcacca gacaacataa cgggggctaa
    22081 tcttgaaccc aaacaactca agaatagccg cgaaacgttg tcattacaac acaggcggct
    22141 atatgacgct cgcagagctg ggcatggcct tctggcatga tttagcggct ccggtcattg
    22201 ctggcattct tgccagtatg atcgtgaact ggctgaataa gcggaagtaa cgtgtcatgc
    22261 gggcgtcagg ctgccgtaat ggcaatttgc gcccggatca ggccgcagag gttaaactct
    22321 gcggcctttt tcgtgcttac tgcgggtaag gcacccagtc gccgccgttc aggcgaacgt
    22381 acggtttatc ctggtattga ataactactg catttgagtt ctcggagacc ggtgctgttt
    22441 gtggcaaccc actggtgagt tttttccagt caacattgtc ttcggtgaaa atcttgccat
    22501 caagaacgcg aaccaccaga tcggagatag ccaggaagct gctcggttgg tcaatgacaa
    22561 tcggtgcccc ctgatgtggt gccttcatgc cgaagaattt caccccaacg gggacgtctg
    22621 tgatagacgg gctagggata tcacgtaggc cagatacctg cattctgtcg cccttcagcg
    22681 cgccgccgtg ttccggcacc acgaccacca tcactttacg acccgatttc tccagttcag
    22741 taaagaaggc gtccagttca tcaaagaatt tctgcgcccg cgctttgtaa tccgctgttt
    22801 tgctgacccc cggataatgg ttgccgtcat gcagtggaag cgtgttgtag aacgtggcac
    22861 tacggctgtt tttatctttt tcggtaacgt ccagccagcg gttaagcacg gcggtatcgt
    22921 cataaaccgg cgaaccatca aagcccagca aaataaccgg cagatttgtt tgatccatca
    22981 attcagtctg catgccgcca ttttcgcgaa cttctttcaa aaaaccgccg aactggccgt
    23041 tatgccccat catcaggtgc tgggtaaagc ccagtttcga aaggttatca aacagatagc
    23101 agtcgttatt tgccggttga tacagattag tgtgcgaagt ctgcccgcag ctggcgcgca
    23161 gtaaacggat cgccgccggg ccactgtagg aggtggcgga gttaaagttc ttgaactcaa
    23221 tatcgaaatg cgaccacagt ggatgcgaca tcaacccggc ggcttctata tccgaccagg
    23281 aaagcgaaca gatgttaatc accagtagtt caaatggctg agcatcagcg ggcagcgaag
    23341 acgggaaggt cgatttacgt tttgcctccg cgttatagaa attattcagc caggcgttaa
    23401 ggttcgccgt tgttggcggt gcagtttgtg cgggcatatc acccactacc ggtgcgccac
    23461 ccgtcgccgc aacggttgcc gctgcgttac cacccgtcgt tgttacagtg gtcgtcggtt
    23521 gtccggctgg ccacaaggag aaacttggtc ccgccagggt aagtacgttc agccatagca
    23581 gtatggcaac cacaaaaacg gtaatgcgaa tccattgtga caggaataac caggccacta
    23641 ataaaacaaa aatggcccca atcatctgcc agttaataaa gcgtgtgaca aggtcgatta
    23701 aataatcggt actgaacccc gccacctgcg aaccctggct cattatgctt tccgggccag
    23761 gcaaccaggt gtcatgccag aacaaagcaa agccgatcgg cagggcaatc cagtggcgca
    23821 agcgatgcag gctgtagcgc ggaatgggca tcagcagaaa cgcggcaaac accaaattga
    23881 ggagcggatg gaagttaaga tatcccgccc acaacaggcc gaacttaacc agaaaataga
    23941 agttccagcc ggaaaggccg cgccagtatt gccagaggga agaaggcatg gcggtatttt
    24001 gcgtaaattg agtcattttt tggttgccct ggctttttcc gtgcggcgta acgtcccggc
    24061 cggtttaaca taacgaggtt tagcaaagaa caaacgtaag gtgtcgcgaa tgcgtcgcaa
    24121 agagtgccgc gccagatagc ccagcgggaa aaatatcagt gcgcaaacga caataatttc
    24181 aatgatatcg ctgatggtca tcatgatgag cgctccacag catcatctaa cagtcgcatg
    24241 ggttctggta ttcgtcgcca gtggcgacca tcgtgctcgg cattgatgac cggtttagaa
    24301 ctttgcgtta aaggcagcgg catgccccat tgttctgggg caagcaagcg catctgcacc
    24361 agctcggcac tgatttgatc atcttcaaac cagaccatac ggtttgagaa aatgtcgcca
    24421 gtaggcaatg ggaaaatatg attcaacgcg gtatccagat cgttaatccg acagaatgag
    24481 agaaacagca ccagccgatt accgccaatg gtcatgatat cgccggtacg gttagggcga
    24541 cacagcgtca gggcttgttc aacgcggata cccggtaccg gacgtagggc aaccagaacg
    24601 cctttaccgt gggcaggtaa tagagggtta ttcatcatgt tgttgacggc attacagaac
    24661 acatcccact tctggaaacc acgcagtttg agcggctggg tcattgacag caaggtagtg
    24721 atatcttccg gcacatagcg actaaacttc tgcccttgca cgctttcgat catcgtcaga
    24781 caacgggaga gtggcgcatt ccacggaata accatatttg caccgcaggc caataacaaa
    24841 cgttcatcgg tggcgcgcag gctagcggta ttttcccgca cgaggatttt catcgcacta
    24901 ccgcgctggc gacgcagggt atgaatgctg cgggccagtg gctcgatttg cgcattttgc
    24961 tgtaaagaaa agaccaccgt cgccgcctga gcggtacggg cttcattgaa caggacttcg
    25021 ttattgttga acagttgcca gtgttccgat agcggcggcg caccttccag tacagcaaca
    25081 ttactcagaa tgcgtttttc gtcgctgcgt ggttggatct ccgcctcttc gctttgaact
    25141 aatgtccaga taccattttg ttgctgaacg ctaagctgct gacgggcgct gacccctttt
    25201 tcgttgcacc agaaggcaat atccagcaaa tgttggtcac cctgaaaacg caaactggca
    25261 agaccaaaaa gtgaacggta ttcctcaagc aacaatgaaa attgtttatc gttattattt
    25321 ccgggattaa ttaccaaaag cgaacaatgg tttaacctgc tccatttatt cattttatcc
    25381 aaccatgagc gaagccgctc ggcaggaatg ttttgccatg cgttatttgc gcaaacaaga
    25441 ataaaaaggt aattatgggg atcaatagaa cactgcaaat c
//
LOCUS       DBANUU010000063        25473 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000063 DBANUU010000000
VERSION     DBANUU010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.063"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            149..652
                     /gene="ftnB"
                     /locus_tag="ACIE68_002528"
     CDS             149..652
                     /gene="ftnB"
                     /locus_tag="ACIE68_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFX5908833.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(731..982)
                     /gene="yecJ"
                     /locus_tag="ACIE68_002529"
     CDS             complement(731..982)
                     /gene="yecJ"
                     /locus_tag="ACIE68_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFX5908834.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1097..1183)
                     /gene="azuC"
                     /locus_tag="ACIE68_002530"
     CDS             complement(1097..1183)
                     /gene="azuC"
                     /locus_tag="ACIE68_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFX5908835.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1446..1769
                     /gene="yecR"
                     /locus_tag="ACIE68_002531"
     CDS             1446..1769
                     /gene="yecR"
                     /locus_tag="ACIE68_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFX5908836.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1940..2437
                     /gene="ftnA"
                     /locus_tag="ACIE68_002532"
     CDS             1940..2437
                     /gene="ftnA"
                     /locus_tag="ACIE68_002532"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFX5908837.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2475..2714)
                     /gene="yecH"
                     /locus_tag="ACIE68_002533"
     CDS             complement(2475..2714)
                     /gene="yecH"
                     /locus_tag="ACIE68_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFX5908838.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            2905..4116
                     /gene="tyrP"
                     /locus_tag="ACIE68_002534"
     CDS             2905..4116
                     /gene="tyrP"
                     /locus_tag="ACIE68_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFX5908839.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4178..4843)
                     /gene="yecA"
                     /locus_tag="ACIE68_002535"
     CDS             complement(4178..4843)
                     /gene="yecA"
                     /locus_tag="ACIE68_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFX5908840.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5039..5125)
                     /locus_tag="ACIE68_002536"
     tRNA            complement(5039..5125)
                     /locus_tag="ACIE68_002536"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5089..5091),aa:Leu,seq:taa)
     gene            complement(5138..5211)
                     /locus_tag="ACIE68_002537"
     tRNA            complement(5138..5211)
                     /locus_tag="ACIE68_002537"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5177..5179),aa:Cys,seq:gca)
     gene            complement(5266..5341)
                     /locus_tag="ACIE68_002538"
     tRNA            complement(5266..5341)
                     /locus_tag="ACIE68_002538"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5306..5308),aa:Gly,seq:gcc)
     gene            complement(5493..6041)
                     /gene="pgsA"
                     /locus_tag="ACIE68_002539"
     CDS             complement(5493..6041)
                     /gene="pgsA"
                     /locus_tag="ACIE68_002539"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFX5908841.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6098..7930)
                     /gene="uvrC"
                     /locus_tag="ACIE68_002540"
     CDS             complement(6098..7930)
                     /gene="uvrC"
                     /locus_tag="ACIE68_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFX5908842.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7927..8583)
                     /gene="uvrY"
                     /locus_tag="ACIE68_002541"
     CDS             complement(7927..8583)
                     /gene="uvrY"
                     /locus_tag="ACIE68_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFX5908843.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8725..8796
                     /locus_tag="ACIE68_002542"
                     /pseudo
     CDS             8725..8796
                     /locus_tag="ACIE68_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            8879..9055
                     /gene="yecU"
                     /locus_tag="ACIE68_002543"
     CDS             8879..9055
                     /gene="yecU"
                     /locus_tag="ACIE68_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFX5908844.1"
                     /translation="MIKIFIGHYINVFYSTVDITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            9042..9266
                     /gene="yecF"
                     /locus_tag="ACIE68_002544"
     CDS             9042..9266
                     /gene="yecF"
                     /locus_tag="ACIE68_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFX5908845.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9333..10055)
                     /gene="sdiA"
                     /locus_tag="ACIE68_002545"
     CDS             complement(9333..10055)
                     /gene="sdiA"
                     /locus_tag="ACIE68_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFX5908846.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(10285..11037)
                     /gene="tcyN"
                     /locus_tag="ACIE68_002546"
     CDS             complement(10285..11037)
                     /gene="tcyN"
                     /locus_tag="ACIE68_002546"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFX5908847.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(11034..11702)
                     /gene="tcyL"
                     /locus_tag="ACIE68_002547"
     CDS             complement(11034..11702)
                     /gene="tcyL"
                     /locus_tag="ACIE68_002547"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFX5908848.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(11717..12703)
                     /gene="dcyD"
                     /locus_tag="ACIE68_002548"
     CDS             complement(11717..12703)
                     /gene="dcyD"
                     /locus_tag="ACIE68_002548"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFX5908849.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(12808..13608)
                     /gene="tcyJ"
                     /locus_tag="ACIE68_002549"
     CDS             complement(12808..13608)
                     /gene="tcyJ"
                     /locus_tag="ACIE68_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFX5908850.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(13696..14247)
                     /gene="fliZ"
                     /locus_tag="ACIE68_002550"
     CDS             complement(13696..14247)
                     /gene="fliZ"
                     /locus_tag="ACIE68_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFX5908851.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(14293..15012)
                     /gene="fliA"
                     /locus_tag="ACIE68_002551"
     CDS             complement(14293..15012)
                     /gene="fliA"
                     /locus_tag="ACIE68_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFX5908852.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYIPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(15176..16507)
                     /locus_tag="ACIE68_002552"
     CDS             complement(15176..16507)
                     /locus_tag="ACIE68_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFX5908853.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSTSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISLAQTTEGALSEINNNLQRVRELTVQATTG
                     TNSDSDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKDGSMKIQVGANDGQTISIDL
                     KKIDSSTLGLNGFSVSKNSLNVSNAITSIPQAASNEPVDVNFGDTDESAAIAAKLGVS
                     DTSSLSLHNILDKDGKATADYVVQSGKDFYAASVNAASGKVTLNTIDVTYDDYANGVD
                     DAKQTGQLIKVSADKDGAAQGFVTLQGKNYSAGDAADILKNGATALKLTDLNLSDVTD
                     TNGKVTTTATEQFEGASTEDPLALLDKAIASVDKFRSSLGAVQNRLDSAITNLNNTTT
                     NLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLSKANQVPQQVLSLLQG"
     gene            16753..18168
                     /gene="fliD"
                     /locus_tag="ACIE68_002553"
     CDS             16753..18168
                     /gene="fliD"
                     /locus_tag="ACIE68_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFX5908854.1"
                     /translation="MASISTLGVGSGLDLSSILDSLEAAEKSTLTPISKQQSSYTAKL
                     SAYGTLKSALESFQTANTALNKADLFTATSTTSSSSAFSATTTGSAIAGKYTISVSQL
                     AQAQTLTTKISQKDSKAAIATSDSVLTIQQGGGKDPVTIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSITANDTGNDNAMKLSVSGDSALESFMGYNGTSGDSSNGM
                     IESVTAQNAKLTVNNVEIENSSNTISDALEDITLNLNDVTTGNQTLTISKDTSKAENV
                     VKAWVDAYNTLQDTFSSLTKYTAVDAGAESQDSSNGALLGDSTLRTIQTQLKTLLANT
                     HSSSNYKTLAQIGITSDASTGKLEIATDKLQTALKKDAAGIGEMFIGDGKSTGVTTGI
                     SSNLTSWLSSTGIIQAAKDGVSKTLNNLTDQYNAASERIDTLMTRYKAQFTQLDVLMN
                     SLNSTSSYLTQQFDTSNSNSK"
     gene            18184..18594
                     /gene="fliS"
                     /locus_tag="ACIE68_002554"
     CDS             18184..18594
                     /gene="fliS"
                     /locus_tag="ACIE68_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFX5908855.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLTPSLIQDPV"
     gene            18594..18959
                     /gene="fliT"
                     /locus_tag="ACIE68_002555"
     CDS             18594..18959
                     /gene="fliT"
                     /locus_tag="ACIE68_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFX5908856.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            19037..20524
                     /gene="amyA"
                     /locus_tag="ACIE68_002556"
     CDS             19037..20524
                     /gene="amyA"
                     /locus_tag="ACIE68_002556"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFX5908857.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(20558..20971)
                     /gene="yedD"
                     /locus_tag="ACIE68_002557"
     CDS             complement(20558..20971)
                     /gene="yedD"
                     /locus_tag="ACIE68_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFX5908858.1"
                     /translation="MKKLAIASALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            21158..22363
                     /gene="yedE"
                     /locus_tag="ACIE68_002558"
     CDS             21158..22363
                     /gene="yedE"
                     /locus_tag="ACIE68_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFX5908859.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            22360..22593
                     /gene="yedF"
                     /locus_tag="ACIE68_002559"
     CDS             22360..22593
                     /gene="yedF"
                     /locus_tag="ACIE68_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFX5908860.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            22700..23341
                     /gene="yedK"
                     /locus_tag="ACIE68_002560"
     CDS             22700..23341
                     /gene="yedK"
                     /locus_tag="ACIE68_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFX5908861.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKN"
     gene            complement(23411..24619)
                     /locus_tag="ACIE68_002561"
     CDS             complement(23411..24619)
                     /locus_tag="ACIE68_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFX5908862.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSRLDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            24627..25055
                     /gene="tnpA"
                     /locus_tag="ACIE68_002562"
                     /pseudo
     CDS             24627..25055
                     /gene="tnpA"
                     /locus_tag="ACIE68_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         6342 a         6449 c         6119 g         6563 t
ORIGIN      
        1 tcagacgtta ttcttatttc agatcatcgt cagaattgac tccacgatca catttcggac
       61 cggcagaaag gaattattct gcaaacagta attatggtgt tttgatttat cttgcacctc
      121 tccacttctg gatataagga tattaggtat ggcaaccgct ggaatgcttc tcaaactcaa
      181 ctctcaaatg aaccgcgagt tttacgcatc caatctctac cttcacctga gtaactggtg
      241 ttctgaacag agtctgaacg gcaccgccac tttccttcgc gcccaggcac agagtaatgt
      301 gacccaaatg atgcgcatgt ttaactttat gaagagtgtc ggcgctaccc ccatcgttaa
      361 agccattgat gttcccggtg aaaaactgaa ctctctggaa gaactgttcc aaaaaacgat
      421 ggaagaatac gagcaacgtt ctagtacgtt ggcacagtta gccgatgaag cgaaagaact
      481 gaatgatgat tcaaccgtca atttcctgcg cgatctggaa aaagaacagc agcatgatgg
      541 tctgttgctg caaaccattc ttgatgaagt gcgcagtgcg aaacttgcgg gtatgtgccc
      601 tgtgcagacc gaccaacatg ttctgaatgt cgtgtcacac cagctgcatt gatcatcatc
      661 ggcgctaatg cattgcgccg atgaaggttt tgagaaaccg ctgcctcatc tgtttgaagc
      721 agcggttttt ttaatgggat tcaccctgtg gggtaaactt gagttcaata agcgcgatgg
      781 ctttttggat tgcccgcatg gtgaccgggt ctgcggcggc gggatggtta gtaaagtcga
      841 tattcttcag ttgactggac attttttcac gaacttcaac gggcgcgatt acatcgagaa
      901 catccagaat ttgtttgata accaactggc aagcaaccac atcagaaacc agttcctgat
      961 cggcattcag cggctgggac atcgtaaact cctgatagca ttttgaaagc cgttatagta
     1021 gcggcttcac atcttcagcg atagtcacat ccaccgtcat caggacacaa aaaaacctgc
     1081 cggagcaggt tttttattat cggaacatat tgcctggcgg tacgtctttg aacgtcttgc
     1141 aatagttatt gaacatactt ttcaggattt tgcgcagttt catcgcggca ctccgaccat
     1201 ttgttataca ggtgttattg tctttgcgct tataatatga caaccatcac aaaaatcaat
     1261 ctttatgtga tacaaatcac ataaatactc ctttaatgtt ataaaaatga taatcaaaaa
     1321 acagcccccc tatttctgac acctacagat ggcaagaaat agcgcctgcc aggcgtcttt
     1381 tccggccatt gtcgcagcgc tgtaacgcgt aaaatagtgc tttctcttac tcttctggct
     1441 ggaccatgag acttctgatt ctgactcttt cattaataac gctggcgggg tgtacggtta
     1501 ctcgccaggc ccatgtgagc gaagttgacg cggcaacagg tattgtacgg ttggtttatg
     1561 accaggcttt tttgcagcat gcccataccg atcgttatgt cagtcgcggg atcgccgatc
     1621 gcgcatgcca gcaggaaggc tatacccacg cggtcccctt tggtcagcca gtaggcaact
     1681 gtagcctttt tgcgggttcc ttatgtttga atacggaatt cactttgtca tatcagtgcc
     1741 atcattccgc cttccctgtc tttctctaaa tattttaatc agcgagggga tcttcgctga
     1801 ttaaagaaat agcggtaatg cgttttattc ccattcgtat ttttaataat tgaagtttat
     1861 attttacctt ttgcaaataa taaaataaca aattatagtg acgccacagc aacaaatata
     1921 acctttgtgg agcactatca tgctgaaacc agaaatgatt gaaaaactta atgagcagat
     1981 gaacctggaa ctgtactctt cactgcttta tcagcaaatg agcgcctggt gcagctatca
     2041 taccttcgaa ggtgctgccg cgttcctgcg ccgtcacgcc caggaagaga tgacgcatat
     2101 gcagcgtctg tttgattacc tgactgatac cggcaattta ccgcgtatta ataccgttga
     2161 atctccgttt gctgaatatt cctcacttga tgaattattc caggaaacct ataaacacga
     2221 acaattaatc acccagaaaa ttaacgaact ggctcatgct gcaatgacca atcaggacta
     2281 cccaacattt aatttcctgc aatggtatgt ttctgagcag catgaagaag agaaactgtt
     2341 caaatcgatt attgataaat taagcctggc aggcaaaagc ggcgaaggtc tgtattttat
     2401 cgacaaagaa ctctctaccc tcgacacaca aaactaatgc tctccggcgg caggtttact
     2461 gccgccgttt caaatcagtg acggcaaatc ttactctgat cggtcgaaaa accgtctttc
     2521 gaaggtataa atttgccttt tgctgccaga aacgctacca gctctcccgc tgtcatccct
     2581 tctgccgagc aggtgtgaaa acgtgcctgt tcaccaaaac gcgctttaat cgcagcttcc
     2641 agactggcat gcgtatattg ctcgcctgat tcaatcatca tatttaacac ttcatgaccg
     2701 tgaatagagt ccatcgtccc tcctcaaaaa aagcctagcg tagcgattgc cgcttatgaa
     2761 gactttgcgc cagcgcagga ctgaatgctt tttattgtac atttatattt acaccatatg
     2821 taacgtcggt ttgacgaagc agccgttatg ccttaacctg cgccgcagat atcactcata
     2881 aagatcgtca ggacagaaga aagcgtgaaa aacagaaccc tgggaagtgt ttttatcgtg
     2941 gcgggaacca caattggcgc aggcatgctg gcaatgccgc tggctgcggc cggtgttggt
     3001 tttagcgtta cgttaatctt gttgattggg ctttgggcgt tgatgtgcta cacggcgcta
     3061 ttactgctgg aggtgtacca gcatgttccg gcagataccg gtctaggcac gctggcaaaa
     3121 cgctatctgg gacgctacgg tcaatggctg acgggcttca gtatgatgtt cttaatgtat
     3181 gctctgactg cggcatacat cagcggtgcc ggtgaattgt tggcctccag catcagcgac
     3241 tggacaggta tttcaatgtc ggcaaccgct ggcgtgctgt tgttcacttt tgttgccggt
     3301 ggcgtggttt gtgttggaac atcgctggtc gatttattta accgttttct gttcagcgcc
     3361 aaaattattt ttctggtggt gatgctggta ttgctactac cgcatattca caaagtgaat
     3421 cttttaaccc tgccgttgca acaggggctg gctctgtcag caatcccggt gatttttacc
     3481 tcatttggtt ttcacggtag cgtgccgagt attgtcagct atatggatgg caacgttcgt
     3541 aagctacgct gggtgtttat aaccggtagt gcgatccccc tggtggcata tattttctgg
     3601 caggtggcaa cgcttggcag cattgattca acaaccttta tgggattgct ggctaatcat
     3661 gctggattaa acgggctgtt acaggcgtta cgcgaaatgg tggcctctcc gcatgttgag
     3721 ctggcagtgc atttatttgc tgatttagcc ctcgccacgt catttctcgg cgttgcgtta
     3781 ggcttatttg attatctggc tgatttattt cagcgttcaa ataccgttgg tggacggttg
     3841 caaactggtg caattacctt tctgccgccg ttggcgtttg cactgtttta tccacgagga
     3901 tttgtgatgg cgctgggtta cgccggtgtg gcgctggcgg tactggcatt gattatccct
     3961 tcgctgttga cctggcaaag cagaaagcac aatcctcagg cgggttaccg ggtgaaaggt
     4021 ggtcgtccgg cgctggtggt ggtgtttctc tgtggtattg ctgtgattgg cgtgcaattt
     4081 ttgattgcgg cagggttgtt accagaagtg gggtgatcag atagcctcaa attccttatt
     4141 gggtgccaga attaacgctg acacccaatt tgacctctta atgcaggcag cactgcttaa
     4201 atttcttacc actaccgcac gggcaaggat cgttacgccc cggtttctct tctgctttga
     4261 tcggttgctg aacagctttt tcctgcggat gcgccatcca gtacgcatgt agatcaagcg
     4321 ccgccagtcg aatggcatct acgctctctt caaacgcttc tggcgacatt ttctctaccc
     4381 gctcgaagtt ttcctcagta ccgtgcagcg caatcgcctc cagcgctggt tttaacgaat
     4441 cgggcaacgt tgaccagtca gaaagtgcca cgccccgcat atagccaaag caccactcct
     4501 caacaatcgt cagctcgctg ccatcaactt ctcgcaagcc gaataacggc tcaaactgct
     4561 ccgggaattc gttcagacgc tctgcggtat cggccatatg ttgaaaagcc agattcataa
     4621 agcgcgtcat ctctttctct gacgcccagc gcggcacata gtcagcccca ccccacacgg
     4681 caaccagcca ctgtgccggt tcaatctctt gcggagaact caacaccgcc gtcaataaac
     4741 cgtccagctc cgccacatca aggatggcgt ggtcagtgtt gtatttggtc agaatatcgt
     4801 ccagccattc caactcactt tcgtttaacg gtcccgtttt catacgcttt tccttgtgga
     4861 tctcaactcg ccagcaccta tcttacatgc cggtccgtat cagagatact ttttgagtgg
     4921 ctttgctggt gattaaaaat taaggagggt gtaacgacaa gttgcaggca caaaaaaacc
     4981 acccgaaggt ggtttcacga cactgcttat tgctttgatt ttattcttat ctttcccatg
     5041 gtacccggag cgggacttga acccgcacag cgcgaacgcc gagggatttt aaatcccttg
     5101 tgtctaccga ttccaccatc cgggctcggg aagaaagtgg aggcgcgttc cggagtcgaa
     5161 ccggactaga cggatttgca atccgctaca taaccgcttt gttaacgcgc caaattcttc
     5221 aggcctttca gccagacatc cgcttgaggc cgatgtcttt taaactggag cgggaaacga
     5281 gactcgaact cgcgaccccg accttggcaa ggtcgtgctc taccaactga gctattcccg
     5341 cattcatcaa gcaatcagtt aatcacttga ttttattatc gtctggcaat cagtgccgcc
     5401 gttcgatgcg ttgcattcta cttacctggc gcgatgagtc aacgatattt ttcaccactt
     5461 ttgatcgttt gctgaaaatt acgccgaaac gatcactgat caagcaaatc tgcacgcgca
     5521 gcgctcaaat attgcaacat tgaccacaga gtcagtaccg cagccacaaa gaaaagtgca
     5581 ataccggcgt actcaaccca aatgttcgga cgccacagca gccaggccaa cgccaccatc
     5641 tgggcagtgg ttttcacttt cccaatccag gagacagcca cgctactgcg tttacccaac
     5701 tccgccatcc attcgcgtag cgcagaaata ataatttcac gggcgatcat cgttgccgcc
     5761 ggtagggtca cccaccagct gtgataatgc tcggttacca gcaccatggc gatagccacg
     5821 agaactttat ctgctacagg gtcaaggaaa gcaccaaacc gggtactctg gttccagcgg
     5881 cgtgccagaa aaccatcgaa ccagtcagtc accgccgcga cgcagaaaat gagcgcggcg
     5941 gcaaacggcg accaggtgac aggcagataa aagaccaata caaagaatgg gataaggatg
     6001 acacggaaca gtgtaagcaa cgtagggata ttaaattgca taatgacggg taactatctg
     6061 ttgtcagtaa gattacccct atgttgctac agagacatca atgtttcaac gaccagaaga
     6121 tcttttctgc cagaccttgc gaaatacccg gcacttttgc aatttcctcg acgctggcgt
     6181 tacgtaaacc ttgcaaaccg cccatatatt tcaacaacat ttgccgacgt tttggcccga
     6241 cgccttcaat ggtttccagg gagctggtat ttttgacctt cgcccgtttt ttacggtgcc
     6301 cgccaatcgc gtgatcgtgt gattcatcgc gaatatgctg gataacatgc agcgcgggag
     6361 aatctggcgg caaactgaat ccctcacctt ccggctcaaa gaacagtgtt tccagcccag
     6421 ccttacgatc tgctcctttg gcaacgccaa gtagcagtgg atgattttta tcccatgaga
     6481 catccagttc ggcgaagaca tttttcgcct gcgcaagctg gcctttgccg ccgtcgataa
     6541 ggatcacatc cgggatctta ctgtcgtcaa tggctttacc ataacgccga cgcagcacct
     6601 gattcatcgc cgcataatca tcgcccggcg tgatgcctgt aatgttatag cgccgatact
     6661 ccgcacgcag cgggccgtta gcatcaaaca ccacacagga agcgacggtt tgttcgccca
     6721 tggtatggct gatgtcaaag cactccatcc gcttcacttc cggcaatttc aacacgctgg
     6781 caagcgcggt cagtcgctgg tgaacggtag attgctgcga aagtttgctg gttaaggccg
     6841 tcgccgcatt ggtgcgcgcg agtttcagat aacgcgccct atcgccgcga ggtttggttt
     6901 gaacattaat cttgcgtccc gccagttctg aaagggaatc ggcgagcagc gttttatcgc
     6961 taagattaaa atcgagcagg atctcacccg gtaaggtgcg catctggctg ccttgtaaat
     7021 agaactggcc tacgaaggtt tctaccacct cgctcagttc cgtaccgcca ggcactttcg
     7081 ggaaatagct gcggctgccg agcactttgc cctgacgaat gaacaatacg tggacacaag
     7141 ccatgcccgc atcgaacgcc acaccaataa cgtcgaggtc gtcgccggta ttggaaacaa
     7201 actgtttttc ggtgacgcgt cgcaccgcct gaatttggtc gcgaatacgt gccgcttctt
     7261 caaactccag attctggctg gcagtttcca tacggctaat cagttgcgta agcacctgat
     7321 catctttgcc agacaaaaac aggcgcacat actcgacctg ctgagcgtat tcttcttcac
     7381 tcaccagtcc ttcaacgcac ggtcccagac agcgccctat ctgatattgc agacacggac
     7441 gcgagcgatt gcgataaaca ctgttttcgc actggcgaat ggggaaaatc ttttgcagta
     7501 gcgccagtgt ttcacgtacg gcatagccat tcgggaacgg gccgaaatat tcacctttag
     7561 catgcttcgc accacgatgc atcgccagac gcggatgggt atcgccactc aggaagataa
     7621 agggataaga tttatcatcg cgtagtaaaa cgttgtaacg cggctgatag agtttgatgt
     7681 agttgtgttc cagcaacagc gcttcggttt ctgtatgagt aaccgttaca tcaatttgct
     7741 ggatctgggc gaccagcgct tcggttttgc gcgaagcgag gttgctacgg aaatagctgg
     7801 aaagccgttt tttcaggtct ttcgctttgc cgacatagat aaccgtacca ccagcatcgt
     7861 acatgcgata aacgcctggc tggctggtta cggtttttaa aaacgctttt gcgtcaaact
     7921 gatcactcac tgacttgata atgtctccgc attacacaga ccatggcgaa ttgccaggtg
     7981 agtcagctca acatcgccat gaatgtttag tttactgaac atacgatagc ggtagctgtt
     8041 caccgttttc ggactgagat tgagctgttc tgagatctca ttgaccttct ggcccttggt
     8101 gatcatcagc ataatctgca attcacgttc agacaaactg gcaaatgggc tttctgtttt
     8161 ttctggttcg atctggctta acgccatttg ttgagcgata tcagaagcaa tgtaacgctg
     8221 ccctgaatag acagaacgaa tcgcactcac gacttcctgc ggagccgcgc ctttgctgag
     8281 gtagcccgca gcaccggcct gcatgacttt cgctggtaaa gggttttctg tatggacggt
     8341 aagcatgatg attttgacat cagctgtgga acgcgcgatt ttacgcgtcg cctcaagacc
     8401 gccaatgccc ggcatactca tgtccattag caccacgtca acggcatttg tccggcacca
     8461 cttaacggcg tcttcaccgc acgatgcctc accgacgact tttataccct ttatatcttc
     8521 cagaatgcgt cgtatccctg cgcgcaccag ttcgtggtca tcaacaagta gaacgttgat
     8581 caaaggaata tctccagaaa tagggataac gctactgata gttagtcatt cgtatattag
     8641 cggtttttat tgcaactttg aaacgttaaa aatggtgggt attcgatttt tctctcgttt
     8701 ttgtcaattc gattgtccac agttatggtc acgcccgtca ccaacggctt accggatcgt
     8761 ttcacagtaa gcccggcgat gaaatgtgac aaaaattgac tttattcagc aaaaatgaaa
     8821 atcagcccgc tgaatatgta acattaatta acccgaacaa tacgggcaaa aaacattcat
     8881 gataaaaata tttattggtc attatattaa cgtattttat agcactgttg atatcacgct
     8941 caaaaaacaa ccactgctat ttttagcaaa gcttatggta tactccgccg ccttaacatt
     9001 tttcaccgca aattttcatt gcaacatgac gaggaaaata aatgagtacg cctgattttt
     9061 ctactgccga gaataatcaa gaactggcaa atgaagtctc ctgcctgaaa gcgatgctga
     9121 cgctgatgct gcaggcgatg ggacaagctg acgcgggccg cgtgatgtta aagatggaaa
     9181 aacagcttgc gctgatcgaa gacgaaaccc aggctgcagt attttccaaa acggttaagc
     9241 aaattaaaca agcctaccgt cagtaatata aaaccggctg agaacgtgcc tttcagccgg
     9301 tttttgcatc tgacacgcag aaagaaaaga gatcaaatta agccagtagc ggccgcgtaa
     9361 caggcaacct gggtcttatt cggtgcattg atttttttct gcatgttttt ctgatggaaa
     9421 ttgaccgtat tctcagagat tgacaaaatc atcgctatct ctgctgatgt cttcccttcc
     9481 gccgtccacc tcagaatttc tttttcgcgc ttgctgaaat tcatctctgg cgtcatcact
     9541 atttcatcat ttaaacgcat cagagccatc agactttcgc gcaccagtaa ctgcattttt
     9601 aattgcagtt catcactaag aatgggtatt tcgcgcgcgc tgcaacggga aaaggacaaa
     9661 aagcccagcg cccggtttgg cagcattaaa tactgagtga caccgcggcg taaaccatgt
     9721 gcgcgcgcgg cttcccataa cggctgtgct tcgttgaata agtcatcatt ccacattaga
     9781 tggccctgac taaagttttc agggttcagc accggatcaa ttgcgagaaa gttttttgcc
     9841 tgataataac taacccacgc ctcagggtaa ttggtgtaga aagccacttt aggtcgcgtg
     9901 aatggtaccg ggtggcgaac acataacgaa tagtaatcgt actccagctg ctgagcctga
     9961 agctctattt catggtagac ctcttctgcg gcctccatcc tctgaaaacg caacagcatc
    10021 gtgcgacgcc agctgaaaaa atccgtatcc tgcatagtaa accgcaacgc ccctgagagt
    10081 gagtatcatt tataatgata attcaaacta tcacataaat aagatttata tataatttat
    10141 attattcagg caatgaatta cttttgcaag ccatcacatt ctcttatgtt attaatgagt
    10201 tatgctgatt tgttaagcag ttttatcacg cttgaaatag cgtcccgccc cgacaggtga
    10261 atcgtcgggg ctgatttttt cttattattg cagcagaaac ttctcgagga actggcgggt
    10321 gcgaggctgc tggggatcgg caaataacgc ttttgctgcc ccctgctcga ctatccgccc
    10381 ttggtccata aaaatcgccc ggtccgcaac atcccgggca aagctcattt cgtgcgtcac
    10441 aatcaccatc gtgcgctttt cctgcgccag ctgacggatg gtgttcagga cttcgcccac
    10501 cagctctgga tccagcgctg acgttggctc gtcaaacaaa atcacctcag gacgcattgc
    10561 cagcgcacgc gcaatcgcaa cacgctgctg ttgaccgcca gacaaacgac gtggatagct
    10621 ggtttcttta ccggccagcc caactttcgc caacagctcg cgagcgcgcg ccgtggcctc
    10681 ttctttcggt tcgcctttga cgatcaccgg cccttcaata atgttctcca gcaccgtacg
    10741 atgcggaaac aaattaaagt tctggaagac aaacccgacg tgctgacgca actggcgaat
    10801 cagcgatttt tgctgactta atgaacgtgc agtatcaata gtgatatcgc caacggtgat
    10861 cgttcccgct tcgggttgtt ccagcagatt tatgctgcgt agcaacgtgg ttttgccgga
    10921 accactcgga ccgataattg ccaccacttc gccaggcttt acctcgaggt cgataccgtg
    10981 cagcaccgtc tgaccgtgga attttttcac caggttctta acttcaatgg cactcatttt
    11041 ggttctctct cctggcgatt aagttgattc tcaaaatggt tctgcaacgt cgataacaca
    11101 gtcgccatga tccagtagat gagcgaagcc gccagataca tggtgaacac ctccagcgta
    11161 cgcgaggtaa tcaactgcgc ctgacggaac agctccggca cctggattgt cgcggccagc
    11221 gacgtatctt ttaccaggct gataaagctg ttcgacagcg gcggcaacgc cacacgcgcc
    11281 gcctgtggca aaatcgcgcg acgcatggtc tgccacggcg tcataccaat actggctgcc
    11341 gcttcccact gacctttatc aatagaagaa atggcggcgc gcagcgtttc ggcagcatag
    11401 gcggcagtgt tcaacgacag accaatcatt gctgacggaa tgggatctaa ttcgatacca
    11461 aactgcggca ggccgtaata gatcataaac agttgtgcga tgagcggcgt accacgaaaa
    11521 atagagatat aaaaacgcgc cagccagcgc acgggccaga ttggcgacag gcgcatcagc
    11581 gcgagaataa acccgagcag taagccaaaa aacatgccgc cgatactgag ttgcagcgta
    11641 tacccagcac ctttcagcag gaacggcaag gaatcaataa ccagttgtat actttcttgc
    11701 atgagcgttt ccggagctaa acgtggggat gataggcgaa cagcgcaggc gcaccgccgg
    11761 tatgaataaa cagaatcggc ccttcatctt tgaaacgttt ctgactgata ccgtcaatca
    11821 gacccgccat cgcttttccg gtatacacag gatcaagcag aatgccttcc agccgcgcca
    11881 gcaatttcac tgcttccatg ccttcgtcgt tcggcacgcc gtagccaggt gcaaaatagt
    11941 catcccagag taaaatttcc gctgatgcgg tcagctccag ttctttcgca atcgcctgtt
    12001 gtaggttaac cactttcggc aattgatcgg caacggaacg cgacacggtc acgccaatca
    12061 gttcgctttc aggcatcagg tgttccagcc caacagccag tccggcgtga gttccggcac
    12121 tgcccgatgc gaccaccacc gacgaaatat taaccgcccc ttcacactgt tgcgcaattt
    12181 ccagcgcact ctccacataa cccagcgcgc ccagagcatt agaaccgcca accggaatga
    12241 catacgggcg aaagccttgt gcttcgactc gcgtcgccag ctcttccagt tgggcattgg
    12301 gatcggtcag tgcgtcgcac atttcaatct gggtattgaa cagatccagc aacaaacgat
    12361 tgccgttggt taaatagttt tctgcggttg tgccaatagg attttccagc agcgccacgc
    12421 agtgcagacc gagtttcgcc gcaactgcgg cagtctggcg cacatggtta gactggatcg
    12481 ccccggcagt aatcagcgta tcggcacctt cacgcagagc atctgccgcg agaaattcca
    12541 gcttacgtaa tttattgccg cccattgcca tgggtgtgac gtcatcccgt ttgatgaaaa
    12601 tttcccgtcc aagataatca gaaaagcgcg gcagatattc gagcggcgtt ggcgcgccga
    12661 taaactccaa ccgtggaaaa cgggttaaat tatgcagtgg cataacagcc tccgatgtgt
    12721 gttgttgtga ttttcttatt atgcacgctg aaaacgcgta aataaaaaag gcgctagtga
    12781 aagcgccctt ttttgtcatt atgctgatta tttggtcaca tcagcaccaa accatttttc
    12841 ggaaagagct tgcagagtgc cgtctttttg catttccgca attgcatcat tcaccgcttt
    12901 cagcaggtct tcatttcctt tacgcagcgc cacgccagac tcctgacggg agaatgcttc
    12961 accggttact gccagcgtat cgttggtttt cttcaccaga tccagcgccg ccagacgatc
    13021 aacgaggatc gcatcgatac ggcctacgcg cagatcctga tatttggtcg ggtcatcatc
    13081 ataggtacgc acatcgacgc cctgaacatt ctgccgcagc cactcttcat agttggtgcc
    13141 cagaccgaca cccacttttt tgcctttcag atcagcagct gttttaatgg tgccttcgtt
    13201 accttttttc accagcgcct gaataccgga aatggtgtaa ggggtagaga aatcgtattt
    13261 tttcttgcgc tcgtcagaaa tggtgacctg attaatcacc acatcaatgc gtttagagtc
    13321 cagcgacgcc agcataccgt cccatttggt cggttttagt gacgcctcaa cgccaagatg
    13381 ttttgccagc tgttgggcaa attccacttc aaaaccggtt aatttgccgt catctccctg
    13441 aaaactgaac ggcggataag tgccttccag ccctaccagc agcgtgccgc gctctttaac
    13501 tttattaagc agaccttcat ctgcaaaact tttaacgctc atgcccgcaa ccagcgctac
    13561 ggccatcaca cccatcaatg cctgacgtcc cagatgtgct aatttcatat ttaccccgaa
    13621 tgttgttatg tctgtttgca gtgtagagcc attgtttgta aacacaaaaa caactccgct
    13681 acatcttatt cttatttaat atatatcaga agcaggcagg ctggaggatt tctgcacaag
    13741 tccggtacac gtttgacgct gatagtgttg atacttccgt aatgcgatgc ggtaattgtt
    13801 ggtgctggtg gtgggcagcc acggggcgag gatttcatca aggaaaccgt cctgcagcgg
    13861 atcgagggaa atattttgct cgtgtagatg attccccaaa cggcgcagac gaacgacata
    13921 ttcacgcacc gtaccagggc tcatttcagt ttgctcaaaa agaaattgct taaagccgat
    13981 aatatcgaaa aaatcactct gcgttttgca atgtaaatca ccgcagaatc ggcacaatgc
    14041 cgcccatgat ttttgttcca cttgccagcc attttcatca agcagcatgt ccaggcggga
    14101 aatctcgatt ttattcacta ttttgccgtc gcgaacgagg gtaatgcggt cgagtaattt
    14161 acggcaatgc gcgcaatggg tctggctgtg tttaaagtct ttaagatagc ggcttaatgg
    14221 ccgtcttttc aggtgctgca ccatcattaa gaactcctgg tagtcaaaat taaagtgcgg
    14281 catttactga cgttataact tacccagttt agtgcgtaac cgtttaatgg cctggctgtg
    14341 taactgactg acccgcgatt ccccgacctc cagcaccgcg ccaatctctt tgagattcag
    14401 ctcttcctgg taatagaggg ttaataccag tttttcgcgc tccggcaacg tttcgatggc
    14461 ttccatcacc cgctggcgca gattactgtc cagcagttgt tgtagcggat tttctcgctg
    14521 atgatcatca gtaaccagtt cgatgctatc gccgtgctct tcgcgccact catcgtagga
    14581 gaagagctgg ctgttattgg tgtcgagcaa catttggcga tagtcggcaa tatcgatccc
    14641 taaacgttcc gctacctcag tttccgtggc gttgcggcca agttcctgct ccagttgccc
    14701 tattgcctgt gccacttcac gcgcgttgcg tcgcacgctg cgcggcaccc agtcacggct
    14761 gcgaagttca tccagcatag cgccacggat acgctgcact gcgtaagttg taaatgccgt
    14821 tccttgtagg gcgtcatagc gttcgacggc attgagtaac ccaatgccgc ccgcctgtag
    14881 cagatcgtca agttccacgc tcgcgggcag tcgaacctgc aggcgcaatg cttcgtgacg
    14941 caccagcggg atataacgct gccacagcga gtgtttatcc attacacctt cagcggtata
    15001 gagtgaattc acgataaaca gccctgcgtt atatgagtta tcggcatgat tatccgtttc
    15061 tgcagggttt ttaatcggac gattagtggg tgaaatgagg ggttatttgg gggttatcgg
    15121 taaattgcgg acagaaaaaa ccccgccggt ggcggggtca ggcaggttaa ggccattagc
    15181 cttgtaacag tgacagaact tgctgcggta cctggttcgc tttagacagc acggagttac
    15241 ctgcctgctg gatgatctgc gctttcgaca tgttggacac ttcggtcgca tagtcggcgt
    15301 cctgaatacg ggactgcgct tcagacaggt tggtggtggt gttgttcagg ttggtgatag
    15361 cggaatcgag acggttctgc acggcaccta gagaagaccg gaatttgtcg actgatgcaa
    15421 tagctttatc cagaagcgcc agcggatcct cagttgaagc accttcaaat tgctcagtcg
    15481 cagttgtggt taccttacca ttagtatcag taacatcact taaattcaga tcagttaact
    15541 taagagctgt tgctccattc ttaagaatgt ctgccgcatc accagcagaa tagtttttgc
    15601 cttgaagtgt gacaaaacct tgagctgcgc cgtctttatc tgctgaaact ttgatcagct
    15661 gacctgtttg cttggcatcg tcaacaccgt tcgcataatc atcataagta acatcaatgg
    15721 tgtttaaggt tactttacct gaagcggcat taacagaagc agcatagaag tctttacctg
    15781 actgaacaac ataatcagct gttgccttac catctttatc aaggatgttg tgcagcgaca
    15841 ggcttgacgt atcggaaacc cccaatttgg ctgcgattgc tgcagactca tcagtatcac
    15901 cgaagttaac atcaacaggt tcattgctag cggcttgcgg gatagatgtg atagcattgc
    15961 tgacattaag agagttttta gaaactgaga agccattcag ccccaaagtt gaagaatcaa
    16021 ttttcttcag atcgatggag atagtttgac catcattcgc accaacctga attttcattg
    16081 aaccatcttt ggaaagaaca ttaacgccgt tgaactgagt ctgaccggat acgcggtcaa
    16141 tttcatcgag acgggatttg atttcgtcct gtattgaaga caggtcagaa tcagagttag
    16201 taccggtagt ggcctgaacg gtcagttcac gaacacgctg caagttgttg ttgatttcag
    16261 acagtgcgcc ttcagtggtc tgcgccagag aaataccgtc gttggcgtta cgtgcggcct
    16321 gagtcagacc tttgatgtta gaagtgaagc ggttagcaat cgcttggccc gcagcgtcat
    16381 ctttagcgct gttaatgcgc agaccagaag agaggcgctc gatagaagtc gacagcgcag
    16441 actggttctt gttgatatta ttttgagtga tcagcgagag gctgttggta ttaatgactt
    16501 gtgccatgat tcattttcct gttttcaagt ctacggttta agtattgggc ttccaccctt
    16561 cggcttcgtc gccgtcaacc ctgttatcgt ctggtcactc acaaccttta gaattttttt
    16621 ctagcggtcg aaatactccc cttcctcgtt gctgttcgtg tcattaagcc aaaaaataaa
    16681 tcgtaaactt tgccccgctt acgccgataa ccccggtatt cgttctatac gtcgcatgat
    16741 taaggaattt atatggctag tatttcaacg cttggggttg ggtcggggct ggacttaagc
    16801 agcatccttg acagtctgga ggcggcagaa aaatctaccc tgacgccgat atcaaaacag
    16861 caaagctctt acaccgcgaa actgagcgcc tacgggacgc tgaaaagtgc actagagagt
    16921 ttccagacag ccaacacagc actgaataaa gccgatttat ttaccgccac ttcaaccacc
    16981 agcagttcat cggctttcag cgccaccacc acgggcagcg cgattgccgg gaaatacacc
    17041 attagcgtta gccaactggc gcaggcacaa acgctgacca caaaaattag tcaaaaagac
    17101 agtaaagctg ccatcgccac cagcgatagc gtactgacca tccagcaagg cggcggtaaa
    17161 gatccggtca cgattgatat cagcgcggct aactcgtcac tttccggcat tcgcgatgcc
    17221 attaacaacg ccaaagctgg tgtcagcgcc agtatcatca atgtgggaaa cggcgaatat
    17281 cgtctgtcca tcaccgctaa cgatacgggc aacgataacg ctatgaaact tagcgtcagc
    17341 ggcgacagcg ccctggaaag ttttatgggt tacaacggaa cgtcgggtga tagcagcaat
    17401 ggcatgattg aaagcgtcac cgcgcaaaac gccaaactga cagtcaataa cgttgagatt
    17461 gaaaacagca gtaacactat cagcgacgcg ctggaagata tcacactcaa cctgaatgac
    17521 gtcaccaccg gtaatcagac actgaccatc agtaaagaca catccaaagc tgaaaatgta
    17581 gtcaaagcat gggtggatgc ctataacaca ttgcaggata cattcagtag cttaaccaaa
    17641 tacaccgctg tcgatgccgg agcagaaagt caggactcca gtaatggagc attacttggt
    17701 gactccacac tgcgtaccat ccagactcag cttaaaacct tgctcgccaa tactcacagc
    17761 agttcaaact ataaaacgct ggcacagatt ggcattacca gcgatgccag taccggcaag
    17821 ctggaaatcg cgaccgacaa actgcaaacg gcactaaaaa aagatgccgc aggcatcggt
    17881 gaaatgttta taggtgacgg taaaagtaca ggggtaacta ccggcattag tagcaacctg
    17941 accagttggc tctcttccac cgggataatc caggccgcaa aagatggcgt cagcaaaacg
    18001 ctgaataatt tgaccgacca gtacaacgca gccagtgagc gtatcgatac cttaatgacg
    18061 cgctataaag cccagttcac ccaattagat gtgttaatga actcgcttaa ctccaccagt
    18121 agctatctga cacagcagtt cgacacctca aatagcaact cgaaataatc ttcaggagaa
    18181 actatgtacg cggcaaaagg cacccaggcc tatgcacaaa ttggcgtcga aagcgccgta
    18241 atgagcgcca gccagcaaca gctggtcacc atgctatttg atggagtgct gagcgcactg
    18301 gttagagcgc gcctgtttat gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca
    18361 aaagcgatca acatcattga gaacgggctg cgggtgagtc ttgatgaaga gagcaaagac
    18421 gaactaaccc aaaacttgat tgctctttat agctatatgg tcaggcgctt gctgcaagcc
    18481 aatttacgca acgatgtctc cgcagtcgaa gaagtggaag cattaatgcg caatattgcc
    18541 gatgcctgga aagagtcttt actcacccct tctttgattc aggacccagt ctgatgaaca
    18601 atgcaccgca tttgtatttc gcctggcaac aactcgtcga aaaaagccag ctcatgttac
    18661 gcctggcgac agaagaacaa tgggacgaac tcatcgccag cgaaatggcg tatgtgaatg
    18721 cggtgcagga gatcgcacac ctgactgaag aggttgcccc gtccaccacg atgcaggagc
    18781 agctccgccc gatgctgcgc ctgattctcg acaacgaaag caaggtaaag cagttattac
    18841 agattcggat ggatgaactg gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt
    18901 taagtgccta tggcgatcag ggtggctttg tgctggctcc gcaggataac ctcttttgaa
    18961 tcttaaagag ccaatggctc gcgaataatc tgattacggc tacgcttcta atgttcccct
    19021 tgaatggagt cgaagaatgc gtaatcccac gctgttacaa tgttttcact ggtattaccc
    19081 ggaaggcggt aagctctggc ctgaactggc cgagcgcgcc gacggtttta atgatattgg
    19141 tatcaatatg gtctggttgc cgcccgccta taaaggcgca tcgggcgggt attcggtcgg
    19201 ctacgactcc tatgatttat ttgatttagg cgagtttgat cagaaaggca gcatccctac
    19261 taaatatggc gataaagcac aactgctggc cgccattgac gccctgaaac gtaatgacat
    19321 tgcggtgctg ttggacgtgg tagtcaacca caaaatgggc gcggatgaaa aagaagctat
    19381 tcgcgtgcag cgtgtaaatg ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg
    19441 tgaaggctgg acgcgttaca ccttccccgc ccgtgccggg caatactcgc agtttatctg
    19501 ggatttcaaa tgttttagcg gtatcgacca tatcgaaaac cctgatgaag atggcatatt
    19561 taaaattgtt aacgactaca ccggcgaagg ctggaacgat caggttgatg atgaattagg
    19621 taatttcgat tatctgatgg gcgagaatat cgattttcgc aatcatgccg tgacggaaga
    19681 gattaaatac tgggcgcgct gggtgatgga acaaacgcaa tgcgacggtt ttcgtcttga
    19741 tgcggtcaaa catattccgg cgtggtttta taaagagtgg atcgaacacg tacaggaagt
    19801 tgcgccaaag ccgctgttta ttgtggcaga gtactggtcg catgaagtcg ataaactgca
    19861 aacgtatatt gatcaggtgg aaggcaaaac catgctgttc gatgcgccgc tgcagatgaa
    19921 attccatgaa gcatcgcgca tggggcgcga ctacgacatg acgcagattt tcacgggtac
    19981 attagtggaa gccgatcctt tccacgccgt gacgctcgtt gccaatcacg acacccaacc
    20041 attgcaagcc ctcgaagcac cggtcgaacc gtggtttaaa ccgctggcgt atgctctaat
    20101 tttgttgcgg gaaaatggcg ttccttcggt gttctatccg gacctctacg gtgcgcatta
    20161 cgaagatgtc ggtggtgacg ggcaaaccta tccgatagat atgccaataa tcgaacagct
    20221 tgatgagtta attctcgccc gtcagcgttt cgcccacggt gtacagacgt tatttttcga
    20281 ccatccgaac tgcattgcct ttagccgcag tggcactgac gaatatcccg gctgcgtggt
    20341 ggtcatgtcg aacggggatg atggcgaaaa aaccattcat ctgggagaga attacggcaa
    20401 taaaacctgg cgtgattttc tggggaatcg gcaagagagc gtagtgaccg acgaaaacgg
    20461 cgaagcgacc ttcttttgca acggcggcag cgtcagcgtg tgggttatcg aagaggtgat
    20521 ttaaattcat ccccggcggc aagccgggga gatttcatta cggcagtggc gtcggtaacg
    20581 gcgctttatc cagcgcggcg gcacactctg ccgtcgggcg atccacacgc tccatcgtca
    20641 taccatcgta ctcaatggtg ttaccgtcac gctcaacctc gtacagctca cgttttacgg
    20701 taacgttagt gagatcatca gacatcaatg tcaacttgcc cggcacggca atcacccgct
    20761 gccactgacg gcaatccagg gtatcgccct ctttagtgac aatcaggctg cctatcgctt
    20821 ccggactgac cagcgcacgt tgcggccctt tggtctgcca gtagcccgcc agccagtccg
    20881 gcgcgggggt tttcacgaca ttgttatagt tttcgacttc ggcacagccc gccagcaaca
    20941 tgagcgcgct tgcaattgcg agttttttca tcattcgtcc tgcatgagaa gaaaagagga
    21001 ttgtggcatt aaagccctga tgtcgccagc ctttccaggt tcagagagaa atttgatcta
    21061 actattattt ttgtgaatta ttaccccgaa aataaatttg catgatgtat gattcgcgat
    21121 tcttgtttca aaccttctga gttcagaggc tacattcatg tcatggcagc aattcaaaca
    21181 cgcctggttg attaaattct gggcacccat ccctgcggtc atcgcggcgg gtattctctc
    21241 cacttactat tttggcatta ctggcacctt ttgggctgtc acgggtgaat ttacccgttg
    21301 gggcggtcag ctcctgcaac tgtttggcgt ccatgctgaa gagtggggtt attttaaaat
    21361 tatccatctg gaaggatcgc cattaacccg catcgacggg atgatgatcc tcggcatgtt
    21421 tggcggctgc tttgccgcag cgctgtgggc caacaatgtc aaactgcgca tgccgcgcag
    21481 ccgtatccgc attatgcagg ccattattgg cggcattatc gccggttttg gcgcgcgtct
    21541 ggcaatgggc tgtaacctgg cggcgttctt taccggcatt cctcagttct cgctgcatgc
    21601 ctggttcttt gccatcgcca ctgccattgg ttcatggttt ggcgcgcgct ttaccctgct
    21661 gcccatcttc cgtattcccg tgaaaatgca gaaagtttct gccgcctcac cgctgacgca
    21721 aaaaccggat caggcgcggc gtcgttttcg tctcgggatg ctggtctttt tcggcatgct
    21781 gggctgggcg ctgctcacgg cgatgaacca acccaaactg gggctggcaa tgctgtttgg
    21841 cgtcggcttt ggtttactga ttgaacgtgc gcaaatctgc tttacttcag cgttccgcga
    21901 tatgtggatc accggacgta cccatatggc gaaagcaatc attattggta tggcggtgag
    21961 tgccatcggg atcttcagtt acgtacagtt aggcgttgaa cccaaaatca tgtgggcggg
    22021 accaaacgcg gtaattggtg gtttactgtt tggttttggc atcgtgctgg caggcggctg
    22081 cgaaaccggc tggatgtacc gcgcggtaga aggccaggtg cactactggt gggtcggtct
    22141 gggcaacgtg atcggctcaa cgattctggc gtattactgg gatgatttcg ctccggcgct
    22201 ggccaccgac tgggacaaaa tcaacctgct gaaaaccttt ggcccgatgg gtggcctgct
    22261 ggtgacatat ttgctgttgt ttactgcgct gatgttgatt atcggctggg aaaaacgctt
    22321 cttccgccgt gcggcaccgc agactgctaa ggagatcgca tgaaaaatat cgttcctgat
    22381 taccgccttg atatggtggg tgaaccctgc ccttatccgg cagtcgcaac tcttgaggcg
    22441 atgccgcagt taaaaaaagg ggaaatcctg gaagtggtga gcgactgtcc acagtcgatc
    22501 aataatattc cactggatgc gcgtaatcac ggctatacgg tgctggatat tcagcaagac
    22561 gggccgacca ttcgttattt aattcagaag taatccctca ttcccgctgt ggcccacagc
    22621 gggaacatcg ttgcctctga cacatagatt ttacccgccc cctctcctat tcttttcagt
    22681 atgatctcag gaggtttcta tgtgtggacg ctttgcccaa tcccaaacgc gtgaagatta
    22741 ccttgcgctt ctcgcggaag atattgaacg cgatattccc tacgatcccg aacccattgg
    22801 cagatacaac gtcgcgccgg gaaccaaagt tcttctgctc agtgaacgtg atgaacacct
    22861 tcatctggat ccggttttct ggggatatgc tcccggatgg tgggataaac cgccgctgat
    22921 taacgcccgc gtagaaactg cggccaccag tcgtatgttt aaaccgctct ggcaacatgg
    22981 tcgggcaatc tgttttgccg atggctggtt tgagtggaaa aaagaaggcg acaaaaaaca
    23041 gccttatttt atctatcgcg ctgatggaca acctgttttt atggccgcga taggtagcac
    23101 accgtttgag cgcggtgacg aagccgaagg atttttaatt gtcactgcag cagcagacca
    23161 gggtctggta gatattcatg accgccgccc gctggttctg tcgccagaag ctgcacagga
    23221 atggatgcgg caagagattg gcggtaaaga agcatcagaa attgctacca acggctgtgt
    23281 gccagcaaac cagttcacct ggcacccggt atcgcgcgcg gtaggtaatg tcaaaaacta
    23341 gggcgcggag ttaattcaac ctgtttgttg acatcctcca cgccctgaag gacgaggatc
    23401 cctgctacgt tcaggctgtc gcctgaatca tttcggtggg ttcctgcttc aacgggcggc
    23461 ctgactgcac catctctcca caggcaagaa cggcgtgccc cgcagctaaa atgttacgag
    23521 cgccatttac atcggcgttc gctgtatatc cacatacctg gcatctgaat ttactttgtg
    23581 acaggcggtt ttctttcgct gtatgaccac agcacgcgca acgctggctt gtgtacgctg
    23641 gcggaacagc aagcacctga ccgccacgcc agagctgctt gtactcaagc tggcggcgca
    23701 tttcatacca gccctgatcc agtctcgaac ggtttaaacc tgattttgcc cggacattgc
    23761 gccccggctg gctgaccgta cccgctgctg actttgacat gttgctgacc ttcaaatcct
    23821 caatgactat cattgcgtgg tttttgctga cggtcgttgt gactttgtga aggtagtccc
    23881 tgcggatatt tgcgatacgg gaatgcagtc gctgtatttt gcgtttctgc ttctgccagt
    23941 tgttgctgaa tttgaccttg cggcttaact gtcgctgaag tctcgccagc ttcttctggt
    24001 ttttctgaaa actgtttaca ggctcaaaga ctgtgccatc tgacagcgtg gcgagtttag
    24061 ccacgccagc atccagcccg accattgatg ctgaagggtg aaccggagtt gatacttcac
    24121 tttctgtctg aatactgatg taccacgtac cgcaggactg gctgacagtg acatttttca
    24181 caacacccgt gacctgacgg ctattccggt agcgcatcca gcccagtttc ggcagaaaaa
    24241 tacggctgtt ttcctgatcg agcttaacgc cctgcgggta gcggaatgca tcattctgtc
    24301 cccgcttttt gaatcgggga aaagcagccc gcttccggaa gaagtttttg taagcccgct
    24361 caaggtcttt cagtgactgt tgcaatggct gtgagggaga atctttaagc cattgcgttt
    24421 cagtggcatt tttccactca accagccagg aagccatttt cccgtaaggg atgtatttat
    24481 taccggcctc atgattctca ttctgacgtg ccagagcacg attgaaaacg aaacgacatg
    24541 cgcctgcgaa gcgcctcatt tcacactcct gttgaccacc tggtcttaac tggaatttaa
    24601 atgcctgtaa tcgtttcaca ggccaaatga tactttggtc tgtgaaaaaa gaaaccgata
    24661 tccggcgtgg aaggcattgt gttttcctga tgcatggtca cctggtcttt gtcaccagat
    24721 accgacgcca gatttttgac catgacgcga cagaaaaact acgcacttac ttttcaaatg
    24781 tatgtgctta ttttgaagct gaactggttg aaatggatgg cgaaccagat cacgtccatt
    24841 tgttaattaa ctaacctccc aaactggcga tatccagtct ggtaaacagc ctcaaaggcg
    24901 tatcgggtag gtgactgcga cgagatcgac cagatattgc agtcaggtat tactacaaag
    24961 gcgttttgtg gagtgctggc tattttgcca gtagctgcgg aggtgcgcca atatccgtca
    25021 tccgccaata cattgaacaa cagcaaacac ctggtcaggt ggaaaaccgc gccttatatc
    25081 cctgccctga aggacggggt tttacggcgc accggataag cctggagatt atctacacca
    25141 gcatggacat cattgtacta tgccccgtac atgccccatc acatcctcaa catcaaagta
    25201 ttagagcctt gcaaaccatt atgcgtctca gtttttctca tctatcttac gcaaaaaaaa
    25261 atagaactaa tatcaagcgg cgctatattg cgtattaaat aaaaccacct caattacatt
    25321 aataaatcta aacaaacatc atgcaaataa tttccatggt tatttctagg ctatttaaat
    25381 taaataaatt atgagtcaat cataaataac aacctttaat ataatttgac taccaaattt
    25441 atattcgttc acaaaaatag tcatgcattg tgt
//
LOCUS       DBANUU010000064        25465 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000064 DBANUU010000000
VERSION     DBANUU010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.064"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(152..2113)
                     /gene="topB"
                     /locus_tag="ACIE68_002563"
     CDS             complement(152..2113)
                     /gene="topB"
                     /locus_tag="ACIE68_002563"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFX5908863.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRALL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(2118..3161)
                     /gene="selD"
                     /locus_tag="ACIE68_002564"
     CDS             complement(2118..3161)
                     /gene="selD"
                     /locus_tag="ACIE68_002564"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFX5908864.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(3278..3829)
                     /locus_tag="ACIE68_002565"
     CDS             complement(3278..3829)
                     /locus_tag="ACIE68_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFX5908865.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            3990..5846
                     /gene="sppA"
                     /locus_tag="ACIE68_002566"
     CDS             3990..5846
                     /gene="sppA"
                     /locus_tag="ACIE68_002566"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFX5908866.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSSSDTKETAGRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKDFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITKALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDTFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6013..7029
                     /gene="ansA"
                     /locus_tag="ACIE68_002567"
     CDS             6013..7029
                     /gene="ansA"
                     /locus_tag="ACIE68_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFX5908867.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDEYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            7040..7681
                     /gene="pncA"
                     /locus_tag="ACIE68_002568"
     CDS             7040..7681
                     /gene="pncA"
                     /locus_tag="ACIE68_002568"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFX5908868.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7774..9132)
                     /locus_tag="ACIE68_002569"
     CDS             complement(7774..9132)
                     /locus_tag="ACIE68_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5908869.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFVIETRKVSLEEISE
                     VN"
     gene            complement(9249..10007)
                     /locus_tag="ACIE68_002570"
     CDS             complement(9249..10007)
                     /locus_tag="ACIE68_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFX5908870.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(10144..11124)
                     /gene="ydjG"
                     /locus_tag="ACIE68_002571"
     CDS             complement(10144..11124)
                     /gene="ydjG"
                     /locus_tag="ACIE68_002571"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFX5908871.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALIINLSDADATLMREMAEALER"
     gene            complement(11134..12081)
                     /locus_tag="ACIE68_002572"
     CDS             complement(11134..12081)
                     /locus_tag="ACIE68_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFX5908872.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFSRFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KTRQMIICADMIKPRLNETLDDICEALSYVDYLFPNYAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(12086..12922)
                     /locus_tag="ACIE68_002573"
     CDS             complement(12086..12922)
                     /locus_tag="ACIE68_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFX5908873.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMARKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            complement(12943..13986)
                     /locus_tag="ACIE68_002574"
     CDS             complement(12943..13986)
                     /locus_tag="ACIE68_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFX5908874.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLIMAEQLGATVVI
                     NGTKEDTIARCQQFTEDMGADIVFETAGSAVTIKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(14003..15382)
                     /locus_tag="ACIE68_002575"
     CDS             complement(14003..15382)
                     /locus_tag="ACIE68_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5908875.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIVVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            complement(15409..16485)
                     /locus_tag="ACIE68_002576"
     CDS             complement(15409..16485)
                     /locus_tag="ACIE68_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFX5908876.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(16855..17127)
                     /locus_tag="ACIE68_002577"
     CDS             complement(16855..17127)
                     /locus_tag="ACIE68_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFX5908877.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(17169..17582)
                     /gene="msrB"
                     /locus_tag="ACIE68_002578"
     CDS             complement(17169..17582)
                     /gene="msrB"
                     /locus_tag="ACIE68_002578"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFX5908878.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            17924..18919
                     /gene="gapA"
                     /locus_tag="ACIE68_002579"
     CDS             17924..18919
                     /gene="gapA"
                     /locus_tag="ACIE68_002579"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFX5908879.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            19003..19887
                     /locus_tag="ACIE68_002580"
     CDS             19003..19887
                     /locus_tag="ACIE68_002580"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFX5908880.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(19938..20792)
                     /gene="yeaE"
                     /locus_tag="ACIE68_002581"
     CDS             complement(19938..20792)
                     /gene="yeaE"
                     /locus_tag="ACIE68_002581"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFX5908881.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYIGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(20882..21628)
                     /gene="mipA"
                     /locus_tag="ACIE68_002582"
     CDS             complement(20882..21628)
                     /gene="mipA"
                     /locus_tag="ACIE68_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFX5908882.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            22064..23998
                     /gene="yeaG"
                     /locus_tag="ACIE68_002583"
     CDS             22064..23998
                     /gene="yeaG"
                     /locus_tag="ACIE68_002583"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFX5908883.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHVPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            24111..25394
                     /locus_tag="ACIE68_002584"
     CDS             24111..25394
                     /locus_tag="ACIE68_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFX5908884.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNSTAKD"
BASE COUNT         6551 a         6566 c         5943 g         6405 t
ORIGIN      
        1 gttttgtaat aaaacttttt tcatcacatt ccctgttatt acatacttta gaggcgataa
       61 ttttatccat gcaaaaaaaa tatccaacga attttcttga tccgctggat atttcagaat
      121 atgactcgaa tagcacgaaa gattcgctcg cttacgctat cgccccgctt ccggcttcat
      181 ctgctggcgg actttttttc gcactacgtt tacgcggtgc agcctttttc ttatcagcac
      241 tgccaccact gcccggagcc acaatgccgc gaaactgccg caccggagta cgtttggctt
      301 gatcaataag ctgatatagc gtccccacca gcggctgcat aaagtcctga tagcgacact
      361 gcttttcgct gatttgcgtc agcaccgatt cccagtgcgc ggtcatgtcc ggtcgcgtag
      421 ccatttctgg cagcgaatgg aataacgctt ttccggcgtc ggtggagtgg atatagcgcc
      481 cttttttggt gaggaaacca cgcttgaaca acagttcaat aatcccggca cgcgttgctt
      541 ctgtccccag accatcggtc gcacgaagga tctttttcag atctttgtcc tgcacaaagc
      601 gcgcaatccc ggtcatcgcc gaaagcagtg ttgcatcggt aaaatggcgc ggcggctggg
      661 tttgccgctc taccacttca cctttttcac acagtaactc atcgcctttc gccaccacag
      721 gcaatggcgt gccgtcgttt tcttcatcgc gctctttgct gcctaacagc gcgcgccaac
      781 ctgcttcagc aagaaaacgc gctttagcga caaatttgcc tttggcaatg tccagttcga
      841 taacacactt gcggaacacc gcatccgggc agaactgcat cagatactga cgggcaatca
      901 ggttatagac cttcgcttcg ttctccgtca ggttgatcgc agaactccgt gcggtcggaa
      961 tgatggcgtg gtgcgcatcg acctttttgt catcccaaca gcggttgcgt atatctggat
     1021 ctaccactgg ctgcggcaac agatctggtg catgaacgct gatggcattc atcaccgcgt
     1081 ggcgtccggc aaaatgttct tctggcaaat agcgacaatc ggaacgcgga taagtgatta
     1141 acttgtgtgt ttcgtacagc ttctggcaga tatcaagcac gttctgcgcg ctcagaccaa
     1201 agcgttttgc cgcttcaatc tgcaacgccg aaagcgaaaa cggcagcggc gcggattctg
     1261 attcccgttt atcgttatag ctggtgacaa tagccggttg accactaatg cggttaacca
     1321 catgctccgc cagtggacga tgtaacaagc gcccttcttc atcctggtac ggttcacacg
     1381 cttcgctcgg ttgccagata gcggtaaacc gctcatcggc aggtgtcacg atatgcgctt
     1441 tgacttcaaa gaagtctttc gccacgaagt tttcaatctc ttcatcgcgg cgcaccacca
     1501 gcccgagcac gggtgtctgc acgcgtccca cggaaagtac gccctgataa ccggcattgc
     1561 gaccgagaat ggtatacgca cgggtcatat tgatgccgta cagccagtcg gcacgcgctc
     1621 gcgccagcgc agaaacgcac aatggcacaa actcactgtt ggaacgaaga cggtcgatcg
     1681 cccgctcaac cgcctgcggg ttcaggtcgt ttatcaagca acgctgcacc tgctggcgct
     1741 tttccggtgc cagttgcaga tagtccagca cttcatccac cagcaattgc ccttcacgat
     1801 ccgggtcccc ggcgtgaacg atttcgctgg cttcatgcag gaagcgtttg atgacgttaa
     1861 gttgtttggt cacggagggt cggggctgta attgccactt ttccgggaca atcggcaaat
     1921 ccgcaagatt ccagcgcgca tagcggctgt cgtaggcgtc tggctgcgcc tgctcaagca
     1981 ggtgaccgat acaccaggtc accacctgac cattaccgca ctcgataaag ccatcgcctt
     2041 tacggtgcgg tttgggcagg acatcagcaa tggcgcgcgc cagactcggt ttttcggcaa
     2101 taaacaaccg cattgaatta acggatctca accatggcac gaccgccgcg cgctggcacc
     2161 agttcgccaa tcgccgtcag ttcaatgcca aactcggcgg ctgtagtttt aacctcattt
     2221 tctgcttccg gcatgaccgc cagcagcaaa ccgccagaag tttgcggatc gcacagcaga
     2281 tcgcgcactt cacgcggcat ttcacccatc agatgaccgt agctggcaaa gttacgttcg
     2341 gtgccgccag gcactgcgcc caacttaatg tactcttcaa cccccggcaa ttttgggatc
     2401 gcgtcatagt caacgcgcgc ctgcacacct gcgccctgac acatttcgct caaatggccc
     2461 agcagaccaa aaccagtaac gtcggtcatc gcttttacac cttcgatgtt ggcaaaggac
     2521 gcacctgcga tgttcatccg gcacatcact tccgtcgcca gtccctgatg ttctggtttc
     2581 aacagtgatt ttttctcagc cgtggtaaga acgccgatcc ccagcggttt cgtcaggaac
     2641 agtttgcatc cggcttgtgc ggtactgttt ttcttcaccc gctcggtcgg tacgatcccc
     2701 gttaccgcca gaccaaaaat cggctccggc gcatcgatgg agtgaccgcc agccagcgca
     2761 atacccgcct gacggcatgc atagcgtcca ccttcggtta cttcgcgggc aatttctggg
     2821 gaaagtttgt taatcggcca gccgaggatc gcaatcgcca taatcggttt gccccccatc
     2881 gcgaagatat cgctgatggc gttagtcgcc gcaatgcggc caaaatcgaa aggattatca
     2941 acgatcggca taaagaagtc ggtggtactg ataacgctgg tgccattgcc cagatcgtac
     3001 accgccgcat cgtcgcgggt ctcattaccc acaagcaaat tcggatcaac aaacttcgcc
     3061 tgctcactgt gcaggatggt ttccaacact tttggggaaa ttttacagcc gcaaccagct
     3121 ccgtggctgt attgggtcaa acgaatcgag ttctcgctca tggacatctc ctgtcaatgc
     3181 aatccgggta tggtaaccct cattccgtga agtgataagt gagagtgtct gaattcctgc
     3241 gcctttgctc acaatccaga cagtttcgcg acaattatca gaaataagtt acaaacggcg
     3301 tcgggtccgg gacgttaatc gacgtagatg ctttcaactg cggcgtgccg aggtagagaa
     3361 aaccgacaat tttatcctgc tcacggcaac cgaatgcttc acgcactacc ggactttcag
     3421 ttaatgcgcc actgcgccag atgccgccaa atccttgggc aacggctgcc atttgcatcg
     3481 ccatgaccgc gcatccggca gacatttcct gttcccatcg cgggacttta tgattttctt
     3541 cgcatttcgc caccaccgtg atgatgagcg gtgcgcggaa cggcgcatta cgggctttgt
     3601 cgatagcttt gtcatcacta ccggcagcaa tcgccccctg ttccagtacg gcgctgaaac
     3661 gctcgcgccc ttccccttca atcacaaaaa aatgccacgg ttgcatggac ttatggtccg
     3721 gcgcacgcat acccgcacgc aggatgtttt gcagttgttc acccgttggc gcgggttcag
     3781 ccaggcggga ggcgctacgg cgattgatca atagttcgag tgcatccatt tgattaactc
     3841 ctgtcgtgat atttattcac aaaattaaca cgagagtgga ttttgttaca gcacagtccg
     3901 caattcctgc tgacaagtgc cggttgggtc attacgataa ccacatctat tgcgcctgtg
     3961 acaggtgtga ccttaagttg ggagaataca tgcgaaccct ttggcgattt attgccggat
     4021 tttttaaatg gacgtggcgt ctgctgaatt tcgtccgtga aatggtactt aacctgttct
     4081 ttatttttct cgtactggtt ggtgtgggga tctggatgca ggtcagtagt agcgatacca
     4141 aagaaaccgc cggtcgtggc gcactactgc tcgatatttc cggcgtgatc gtcgataagc
     4201 ccgacagttc gcagcggttt agtaagttaa gccgccaact gcttggcgcc agttccgatc
     4261 gtctgcagga aaactcgctg tttgatatcg tgaacacgat tcgtcaggct aaagatgacc
     4321 ggaacatcac cggtatcgtg atggatttga aagactttgc gggcggcgac caaccgtcta
     4381 tgcagtacat cggcaaagcc ctgaaagagt ttcgtgacag cgggaaaccg gtttatgccg
     4441 tcggcgagaa ctacagccag ggtcaatatt atctcgccag tttcgccaat aaaatttggc
     4501 tgtcaccgca aggcgtggtg gatttgcacg gttttgctac caacggtctg tactacaaat
     4561 cgttgctgga taagctgaaa gtttccaccc atgtgttccg cgtgggtacg tataaatcag
     4621 ccgttgaacc gtttattcgt gatgatatgt caccggcagc ccgcgaagct gacagccgct
     4681 ggattggtga gctgtggcaa aactatctga atactgttgc cgctaaccgg cagatccctg
     4741 ctcagcaggt attccctggc gcgcaagggt tgcttgaggg tttaaccaaa accggtggcg
     4801 ataccgcgaa atatgcgctg gaaaacaagc tggtcgatgc actggcatcg agtgccgaaa
     4861 tcgaaaaagc tctgaccaag gagttcggct ggagtaagac tgataaaaat tatcgcgcca
     4921 tcagttatta cgattacgca ttgaaaacgc cggcagatac cggtgacagc atcggtgtcg
     4981 tcttcgctaa tggcgcaatt atggatggcg aggaaactca ggggaatgtt ggcggtgata
     5041 ccactgcggc acaaatccgc gacgctcgcc ttgacccgaa agtgaaagcg attgtcctgc
     5101 gtgttaatag cccaggcggc agcgttaccg cgtctgaagt gattcgcgct gaactggcag
     5161 cagcccgggc agcgggtaag cctgtggttg tatcgatggg cggcatggcg gcatctggtg
     5221 gttactggat ttccacacca gctaattaca ttgtggctaa ccccagcacc ctgaccggtt
     5281 ctatcggtat cttcggcgtg atcaccaccg tagaaaatag tctggattcg attggtgttc
     5341 atactgatgg tgtctcaact tcaccgctgg cggatgtttc tatcacaaag gcactgccgc
     5401 cggaagcgca gcagatgatg cagttaagca ttgagaatgg ctataaacgc tttatcacgc
     5461 tggttgctga tgcgcgtcat tcgacgccgg agcagattga taaaatcgcc cagggccacg
     5521 tctggaccgg tcaggatgca aaagctaacg ggctggtcga tagtctcggg gatttcgatg
     5581 atgcggtcgc caaagcagca gagctggcaa aagtgaaaca gtggcatctg gaatactacg
     5641 ttgatgaacc gaccttcttc gacaaagtga tggacaatat gtcaggttct gtccgggcaa
     5701 tgttgccaga tacgttccag gccatgttac ctgcaccgct ggcctcggta gcctctaccg
     5761 ttaaaagtga aagtgacaag ctggccgcgt ttaacgaccc acaaaaccgt tatgcgtttt
     5821 gcctgacctg cgccaacgtg cgttaagtct tgtactgagt ggccgacaga tcgtcggcca
     5881 cattattttt tacgtcgacg aatcctcttc ccgctgtctc gccccatatt tccttatccg
     5941 catagtatca ggtgcgctcc ccctgcctca cgtatatact tttgctcttt cgatatcatt
     6001 catatcaata tcatgcaaaa gaaatcaatt tacgttgcct acacgggcgg gaccatcggg
     6061 atgcagcgtt ccgagcaggg ttatataccg gtgtcaggtc atctacaacg ccaactggcg
     6121 ctgatgccgg aattccatcg cccggagatg ccggatttca ccattcatga atatacgccg
     6181 ctgatggatt cttctgatat gacgccggaa gactggcagc atattgctga agatattaaa
     6241 gcgcactatg acgagtacga tggttttgtc attctgcacg gcaccgacac gatggcgtat
     6301 accgcctctg cgctgtcgtt catgctggag aacctcggaa aaccggttat tgtgacaggg
     6361 tcacaaatcc cgctggctga gttacgctct gacggacaaa ttaatctgct gaatgcgttg
     6421 tacgttgcgg cgaattatcc gatcaacgaa gtaacgctct ttttcaataa ccgattgtat
     6481 cgcggcaacc gcactaccaa agcccatgcc gatggttttg atgcgtttgc ctctcctaac
     6541 cttcctccgt tactggaagc aggtatccat attcgtcgtt tgaatacgcc acccgccccg
     6601 cacggtgaag gggaattaat cgttcatcca atcaccccac aaccaattgg cgtagtgacg
     6661 atttatccgg ggatttctgc tgacgtcgtg cgcaattttc tgcgccaacc ggtgaaagca
     6721 ttgattctgc gctcctatgg cgtgggtaat gcgccacaaa acaaagcctt cctgcaggaa
     6781 ttacaagaag ccagcgatcg cggtattgtg gtggtcaacc tgacacaatg tatgtccggt
     6841 aaagtgaata tgggtggtta tgccaccggt aacgccctcg cccatgccgg cgttattggc
     6901 ggtgcagata tgactgtaga agccacgcta accaaactgc attacctgct gagccaggaa
     6961 ctggatactg aaaccattcg caaggccatg agccaaaacc tgcgcggcga actgacgccg
     7021 gatgattaag gagactgcaa tgccccatcg cgccctgtta ctggtcgatt tacaaaatga
     7081 tttctgtgct ggtggcgcgc tcgccgtacc ggaaggtgac agtacggtgg aggttgctaa
     7141 ccgccttatt gactggtgcc agtcgcgcgg agaagcggtt atcgccagtc aggactggca
     7201 cccggcgaat cacggcagtt ttgccagtca gcacggtgta gagccttata cgccaggcca
     7261 actcgacggt ttgccacaaa ccttctggcc agatcactgt gtgcagaaca gtgaaggcgc
     7321 acaattacat ccgttactga accaaaaagc gatcgcagcg gtgttccata aaggcgaaaa
     7381 tcctttagtt gacagttaca gtgccttttt tgataacggc cgtcggcaga aaacctctct
     7441 cgatgactgg ttacgcgatc atgaaatcga tgaattgatc gttatgggcc tggctactga
     7501 ctattgcgtg aagtttaccg tgctggacgc gttacagtta ggttataagg tgaacgtgat
     7561 taccgatggt tgtcgtggcg tgaatatcca gccccaggac agtgcacacg cgtttatgga
     7621 gatgtcagca gctggggcaa cgctatatac gctggcagac tgggaagaga cacaggggta
     7681 attttacgct ggcctacaat tctgtactgg cattgtaggc caaataaaac acgtcagtgg
     7741 tacatctggc aattgatgcc atcaacgaaa gatttaattc acctcagaaa tctcttccag
     7801 cgacaccttc cgcgtttcga tgacaaaaat ggagagaacc agcgcacaga gcactaacat
     7861 gacaccaagc accataaaca ccgtgatcga cccataatgt gttaataatg ccgcaacgcc
     7921 atagggcgtg aaaactgcga cgatccgtcc gacggcatta acgaaacccg aaccgcgcag
     7981 gcgtaaatgc gttggccaaa gctccgggat ataaaccgct gacgcgaagc aaacatacat
     8041 gtataaaaag aagatcatca ccagaccata gattaaaatc gcccactctg tagtctgaat
     8101 tgaatagata tagcctaaca cggcaataat aataagtaag gcggagccaa ataatcgacg
     8161 aggaaaatga tcaataatta atgccgcaat aaatattcct accggagcac caatcataat
     8221 aacagcggtc attaatattg atttatcgac atcaatcccg gagttaacaa atatggtcgg
     8281 tatccatacg gtaatggtat aaagcgaaat gttcattgca attaaaacag ttatcgcaac
     8341 taatgtacgt cgtaacattt cccctttaaa caggagccag aaggtaccct tgattacctt
     8401 aaccttgctg ttgctctggt tcaaagtaag ctggggtaaa cgaatactct tctccctttc
     8461 aatttgctgc tctacttcac gaagttggct ttctgcaccg gcgatttgcc ctttccctgc
     8521 cagccatcgt ggcgactcga taaagtattt acctgagaga aaccaggcta acagtatgcc
     8581 aataccaccc agaagaaaca ttattcgcca actaaaaaaa gcgataacca ccacgcctat
     8641 cgccgcagac agcatgggcg accagttacc aacaaatgag agccgcgcgg accattttcc
     8701 acgcaccgtc gcggggataa actccgtaaa tgaggcatag ccaaccataa tcagcgcccc
     8761 cattcctgtt cccatcagga agcgaaagaa aatgagccag tacatatcag ggacaaaagc
     8821 ggcccctgtt gcagcaatac cgacgatgag aagatttatg cgaaacgccc tgcgccgccc
     8881 aaagtagtca ccaataaacc caccagtaag tgagccgatg aaataaccaa acattaatgc
     8941 cgaggtaaag gcggcattga ggaaattatt tgaccatcca ttgcttacca gctttgctaa
     9001 gacgacatta cctgagtaac tcaaaaaccc cgttaataac agactaaagc ttattatacc
     9061 aaatatacga taatgaaacc gggccaaagg caagcggtcc agtcttgcgc caatttgatc
     9121 atattgttcc attaggtgcc tcggatttgc taaaaaggta gcaattgcgg caaattcact
     9181 tgccgcatct ttatcatcat tttatttcaa ggcgcaggaa caaataatat tgacaacgtg
     9241 tgcttaaatt accacaccag ctggatatta ttgtctttgc aaaatgctat ccactctgca
     9301 cccggtgatt tatcagttat tatgtaatta atatgactaa aatcagctaa ctggacaaaa
     9361 gctttgcgat caaacttaga gtgatcaacc aataacgcaa cttctgtcgc ctgacggatc
     9421 attgtctttt tgatttcagc ttccgcttca ttagagtcca gcgcgccgct gttaatatca
     9481 agacctttac aactcattac catgatatcg acatgatagc ggctgatgat ctctttagta
     9541 attcttcctt gcagtgaaag tgtatttttg tttagttccc cacccgttga aacgacttta
     9601 atttctgact gagccagaac atgaatcgct tccgccgagt tggttagcaa cgtcaggtca
     9661 ctacggtcct gtaacaattt gagcaattcc attacggtac tactggaatc ggctgccatg
     9721 gtggttttat tgtcgataaa gggtagtgcc ttgcgtgcaa taagctgctt ctcttcataa
     9781 aacgatgaag cgcgcttata aaaatggata ttctccgtca acatcgctgt atttaaaaca
     9841 gcaccaccat aggttctggt caaaaagcct tcatcttcca gcttctcaag atcgcggcga
     9901 atggtttctt cggttacctg aaaaatccca ctcaaatttg agactgtcac ttttttatcg
     9961 ttggcaacca tttgcttaat tgcctgaatc ctgtcttttg ccgccacgat tacacccctg
    10021 tatcttttta catcacatta gcgcgattat cgcataaccg atgtttactt tcaaaacaac
    10081 ctgtttgaat cacagatttt catcacagtt ttcacagaaa cagaggtgaa tcgtgttgag
    10141 tatttaacgc tccagggcct ctgccatttc cctcatcaat gttgcgtctg catccgataa
    10201 gttgataatc agtgccgcga cattttcgcg tacctgttcc ggtgcagtag ccccactaag
    10261 aatggagatt aaatcactct gttttaatat ccacgccagt gccagagtgg gaattgtgca
    10321 ctgataacga gcacaaagtg gctgccactg ttcaagcata tcaatcactt tcagcatgtt
    10381 ttcacgctgg aaccagactt tatttgcccg agcgccgccc ggaacgtaat cacgagtgat
    10441 ggtgccggtc aacaatccct gctctagcgg ggaataaacc tgaaccacaa tgccattatc
    10501 acgacatagc ggcagcagtt cgttttccat tgcccggtcg aggatactgt atttcgcctg
    10561 aataatatcc agttcaccat attgcagata ctcgcggata tggtcagcat cgacgttagc
    10621 agcgcctata gcgcgaattt tcccttcagc cttcaactca ttaagcactg cgacagtttc
    10681 agcgatcggc gtaaaaaatg gcggcaccga ctgccagtgc gtcatgtaga tatcgatgta
    10741 atcaataccc agacgttgca agctggcttc tacctcttcg cggatagatt ccggggaaag
    10801 gtttttatac aactgccgat cgccaacttt gttgaataaa cttccttttc gttcccagac
    10861 aatgccgcat ttggtttcta ctacaacctg ttcacggggc agttttttta acgcctgacc
    10921 gacgataact tcactattgc caaagttata tcctggcgca gtatcaatca gattaatgcc
    10981 gcaacgatgg gcttcaagaa tcgtatcaat acatatttgc cgatcgagat cgccattcca
    11041 tgcaggaccg ccgccaatgg cccatgtccc caaccccatt cgcgaaagcg taatatccgt
    11101 tgtgcctaaa ggtatctttt tcatttgcct tcattatcct tcgtattctt ccagcaattg
    11161 ttcgaccagc tttctgtttt ttacgccggt ggtggcaccg acgcttagaa ccgagatagc
    11221 cgccgttgca ttggcaaagc gtgcgcattc acgcagattt ttgccttcta acagtgccgc
    11281 aataaaacct gaagcaaagt tatcgcccgc gccaatggtg tcgatggcgg ttattcctgc
    11341 gaccgccggc accttcatcg tcatgtcacc acgcttgata aagcagccgt ctttacccgt
    11401 tttaatcacc accgttttta cgccgcacgc aagaaagcag tcagcaattt catccaatgt
    11461 ctctttcccg gtgagtaatt ttgcctcggc ataattagga aacagataat cgacataact
    11521 caatgcttcg caaatatcat ccagcgtttc attcaaccgc ggtttgatca tatcggcaca
    11581 gataatcatc tgccgggttt tagcttgcgt aaaaatttct gttagcgctt taccatccaa
    11641 tagaggactg ttgaaaatac tggccagaga taataatttc gcctgagaaa accgcgaaaa
    11701 atcaacatcg tcaatattca atttccacag actgccgtta cggtttgtga caaacgtccg
    11761 ctcgccatcc tccgtcacca gtccaacgtt aatagaggta tctatgctga cgtcctgctt
    11821 caggctttga atatcaatat tctctttgcg gcaatggtcg agaataaact gtcctgcggc
    11881 atctttacca atacggctca ttaatgctgt acgatggccc agacgagaaa taattgttgc
    11941 ttcatttatt gcatcacccc cggtggtcat tgcgattctt tcaagaggat aagaatccac
    12001 atcaaagata tttttactga ccggttgcaa tggaatatca acaatagcgg cacctataca
    12061 aataacgtcg agattatcca tatcattatt ccgctttgcc atcagaaccg aacagtttaa
    12121 ttttttccaa tgcccgttct tttacagctt tacgtacttc acgttctaaa tgcaggaaag
    12181 gctgatcctg gttttctttc actgcgacca ttgcggcctg acagagctcc gtatgaatgt
    12241 tgattttcgc gatacccaac gaaattgcag ttttaatgtc ggcatcactg atccccgatg
    12301 caccgtgcaa aaccagcgga acagaaacgg catcgcgtac gcgtttgacg acctcaaagt
    12361 tcaattgtgg ctctgacgta taaaccccat gctggttgcc gatggcgaca gccaatgagt
    12421 cacagcccgt acgttcaaca aattcagccg cctggtcagg atcggtatag tgatagcccg
    12481 ccagcgcttc ctcataaacg gtttcattac cgacatgccc taattccgcc tctaccggaa
    12541 tacccagcgg atggaagaaa tcgacagcct ctttggttaa acgaatattt tcttcgaaat
    12601 caaatgcaga agcatcacgc attaatgaat tcataccatg agtccaggcg ttatgaataa
    12661 tctccatact ccgaccatga tcccaatgag ttattaccgg cacagttgct tttcgtgcca
    12721 ttgataccat catgtgagag aaatcttcaa atgaggtgtt gccgacaaaa cctgtaccaa
    12781 aagaaataat aaccggtgat ttcgcttctt cggctgcgtc gataacgccc atcaacattt
    12841 ctgcattcca tacgttaaaa tgggcaattg cataatgttt atttgtggca tcgttttccc
    12901 aatatctgat atctgcgagc atattctgtt ctcccgtatt aattaatcgc taattttaat
    12961 aacgccttta ataatgtcgc gtttgttgtt aactgactct tcaaatgcct gttgtacatc
    13021 ccgataatcg taaatatgcg tcaccatcga tttcacatcg aatcgccctg aagaaatagc
    13081 ttcaatcgtg accggataac gattggcata gcggaatacc gtctggatag tgacttcgcg
    13141 attgattttg aggaaattga ttgccgaatc gcccggtaca gtaccaacaa tcataatttt
    13201 accgccgcgc attaccagat aaggtgcctg tttaatggtg accgcagaac ccgctgtttc
    13261 gaaaacaata tctgcgccca tgtcttcggt aaattgctga cagcgtgcaa tagtgtcttc
    13321 ttttgtgccg ttaataacca ctgtcgcacc aagttgttcc gccattatca gacgtttttc
    13381 cagcacatca acgacggcaa tttccgttgc gcccaggcat ttgcacgctt gcaacgtcat
    13441 caaaccaata caacctgctc ccagaataat tatcttctta cccggtttaa catctgccag
    13501 catcgcggca tgcatcccga ctgcggcagg ctccaccagc gccccttcca tcgtgtccat
    13561 attgtctggc agtttgtaag taaagctctc cggatgacac agatagtgcg ttaatgcgcc
    13621 gcggtagttg ggttgtgtcg ccataaaatc aacgtccggg cagatgttat atttgccttc
    13681 cagacagtaa cgacagtgac cgcaaggaac gccaggttcg atatttaccc gatcccccgg
    13741 tttaaatttg cgcacgcggc tacccacagc cacaaccgtc ccggcgcatt catgacccag
    13801 gccaatttct tgatttgggt ctttaggcgg aataaacggg cctgattcaa aaccatgtac
    13861 atctgaacca caaataccga catattctac tttaatcaaa acttcatctt ctttaggcac
    13921 tggtatttct gctgaaataa ttttcattgt gcccggcacc tgcaatattg cttttgaatt
    13981 tttcattttg aatccacccg tattatttat tggctactgc atcaatactt aaactttcaa
    14041 cggagacacc tttggtttca attccaatgg tggcgatagc aattgcgaca acaattgaaa
    14101 cagcccccag aagaataaag actcccgtta cgccataact actgagcagc actgcaacgg
    14161 cataaggtgc ggcaatacca ctgattcgcc ctaccgcatt cgccagaccg gagccacgga
    14221 gtttggcctc tgtcggccag atttcaggga catacactgc cgaggcatag caaacgtaca
    14281 tatagacgaa agtaatcagg aagaaaccaa tcaacgttat gagcaacata ctggtttgca
    14341 gtgaatagat atatccgagc accgcaatca ggattaatag ccccacaccc attgttttac
    14401 gcggaatttt atccatcacc agcatggcaa taaaaatacc aaatggcgcg ccaaacatac
    14461 tcatggtatt taaaacaatc gagtctttta aattaatccc ctgggtcatg aatattgttg
    14521 gcaaccagtt aattagtgtg tactgcacaa cgttcatggc aatcagcaca caagagccta
    14581 atatcacgcg tttcagtaat actcctgtca gtaaggctga atacggcacc gcttgtggcg
    14641 ctttaccgtc atcagcaata accacaggcg gcaaaggttt acccgtctgg cgtatgacgc
    14701 cttcttctat actccgcatg actttctcgg cttcctgata ccgtccgcgc gattccagcc
    14761 agcgcgggga ttcaggaaag tagcgccagg ccagcgccgt agcgataagc gacaatattg
    14821 cagggataag cagttgtacc cgccagttcc actctgcact aatcagcggc gtgagtccca
    14881 tcgctatcaa tgaacagagc ggatatgacc agttgccaat aaaggaaacc cgacttgacc
    14941 acgttccacg atttctaccg ggcatatatt cggtgaaacc agcaaacagc gtaaccagta
    15001 aagctcccag cccaactccc atcacaaaac ggcaggcaat aaggaaatcc atattcggtg
    15061 agaaagcacc gacaaccatc gaggcaatat gaatggcctc atagaggatg aacgcatttc
    15121 tgcgaccggt tttgtcgcca atgatgccgc ccaccagcgc accgagaaac attccggcgg
    15181 tcgtgattgc tgagaatgtg gctgtggtgg aattatctgt ccagcccaac gctttcagct
    15241 gcgcgaggat caagccacca acggcattac tccagcagac aagcaagcca aacgcgacga
    15301 tggcaaacat tgacgaatgc cagcggcaat ccggtaagcg atccagccgt gcaccacaat
    15361 gcggttttgt tatctgttcc atgcgataac gttccttcag gagagtggtt attcatcaaa
    15421 gtcgtaagtc atgatcactt tgattgcggt tttatcgacc atcgcatcaa acccttcgcg
    15481 ccattgcgac aggccgatac gatgcgtgat catcggtttg actttgatag cgccgctggc
    15541 taatagccgg atagcgttac gccatgaggt ggagtcatag gccatatgcc caatgatgct
    15601 tttattccag gcggtaatgt cattaatcga gaaatcaaga ggtttgaagc ccattccaac
    15661 gcgtaccact tccccattcg ggcggagcat ttcgatggct tgtttcagtg cgatattggc
    15721 accggagcat tcaatcacca gtcccagatt atctttgcca caaatttgct ggcagcgcgc
    15781 caccacatct tcggtagaac catttactac tgccgtcgcc cccagttctt ttgcaaccgg
    15841 gaagcggacc gccacatctt cttgcagacc aacgacgacg atatttaccg cccccataat
    15901 ccgcgccatt tgtacggaaa acagcccgag tgggccagtg ccgatgacga caacatcctg
    15961 accaggaagg aatttcgatt gctgcgcgat ggatttatag gcattacaga tagggtcaag
    16021 tacggctgcg tcctcataat caacaccatc agggatttcc cacaacgcat gacgatgaat
    16081 tttgagaatt tcaccaggaa ccagacaata tttggaaaaa ccaccgcccc aggtattgtt
    16141 atccagacca aggtttactt tttctgtaca acacagaaaa tcaccctgtt cacaggccgg
    16201 acaaacgccg caaacgtgac cgctgttatc cgacacgacg cgttgcccca ctttccagtc
    16261 tttgactttt tcaccaacct gcgcaataca acctgcgaac tcatggccgc ggatagagtt
    16321 aaactcatca gaaccgctat cgacattgta gtgcttcatg tctgcgccgc agattgccgc
    16381 ggctttaatt tcaatcacta cgtcttccgg gccacaaatg ggttgtggga catcaatcat
    16441 cttgtagccg ccaaaggcct tgccaaaccg agccagtgct ttcattgcgt catcctctgt
    16501 gttttgtttt gcttagatgg ccatcatact tacccacatg atttttgagg tcaacattaa
    16561 atctgtaaaa cacagataat gatctgcgtt ttacaactta gttcacaatt acgcagaaaa
    16621 tggctgggtc tgttacaggt tggtggaagg cgggacgcaa aaagagcaaa ttcgagaggg
    16681 attaccggcc aaataggtct gagacttaag ttcaggagaa gtcattcagg cgtgaacgcg
    16741 actgcaagag atcggactgg gacttagcca ggtcgggtcg agataattga gacgagtcgt
    16801 ggggtcattg ttggctatcc ccttcatagc cgcggcaaaa aggggatgaa ccacttactt
    16861 aaacatagca ataggctttg ctgagatacc aaaatcttct tttaactgct gtttgctctt
    16921 catgaccatc tgaccatttg tgtcaattgt catatgctgt gcttcggtat tatggcgagc
    16981 ttgccacagc atcaccagtt gcaggcagtt cgctttttgt tcctcggtca acgcaacgcc
    17041 atcgggccat ttccccagtt caacggcggt cgacaaacgc tggtatacct caggcgtcat
    17101 gctgttgata atgtcatcaa gattcataat ttttccgctc ctgtggaata agttgctgaa
    17161 tcgttttttc aaccgttgat ttcttcgccg ttttcgccat cggtaaagcg taaagaggca
    17221 gagttaacac aataacgttc gcccgttggc tgcggcccgt cagggaagac atgccccaga
    17281 tgggcatcac agttaccgca acgaatttct atgcgctgca ttccatgtga caagtctttg
    17341 atataacgaa tggattcttc actcaccggt tcgtagaaac tgggccaacc acagccggaa
    17401 tcatacttgg tttgggaatg aaacagcggg gcatcgcaga tcaaacagtg atatacgccg
    17461 tcacgcttgt tatgcagtaa acgacccgta aatggcggct ctgtcccatg attctgcgtc
    17521 acgtaaaact gcatctcgga caaatttttt ttcagttctt ctgccgaagg tttattagcc
    17581 atttgctcac atctcacttt aatcgtgctc acattacgtg actgattcta acaaaacatt
    17641 aacaccaact ggcaaaattt tgtcctaaac ttgatctcga cgaaatggct gcacctaaat
    17701 cgtgatgaaa atcacatttt tatcgtaatt gccctttaaa attcggggcg ccgaccccat
    17761 gtggtctcaa gcccaaagga agagtgaggc gagtcagtcg cgtaatgctt aggcacagga
    17821 ttgatttgtc gcaatgattg acacgattcc gcttgacgct gcgtaaggtt tttgtaattt
    17881 tacaggcaac cttttattca ctaacaaata gctggtggaa tatatgacta tcaaagtagg
    17941 tatcaacggt tttggccgta tcggtcgcat tgttttccgt gctgctcaga aacgttctga
    18001 catcgagatc gttgcaatca acgacctgtt agacgctgat tacatggcat acatgctgaa
    18061 atatgactcc actcacggcc gtttcgacgg taccgttgaa gtgaaagacg gtcatctgat
    18121 cgttaacggt aaaaaaatcc gtgttaccgc tgaacgtgat ccggctaacc tgaaatggga
    18181 cgaagttggt gttgacgttg tcgctgaagc aactggtctg ttcctgactg acgaaactgc
    18241 tcgtaaacac atcaccgctg gtgcgaagaa agtggttatg actggtccgt ctaaagacaa
    18301 cactccgatg ttcgttaaag gcgctaactt cgacaaatat gctggccagg acatcgtttc
    18361 caacgcttcc tgcaccacca actgcctggc tccgctggct aaagttatca acgataactt
    18421 cggcatcatc gaaggtctga tgaccaccgt tcacgctact accgctactc agaaaaccgt
    18481 tgatggcccg tctcacaaag actggcgcgg cggccgcggc gcttcccaga acatcatccc
    18541 gtcctctacc ggtgctgcta aagctgtagg taaagtactg ccagaactga atggcaaact
    18601 gactggtatg gcgttccgcg ttccgacccc gaacgtatcc gtagttgacc tgaccgttcg
    18661 tctggaaaaa gctgcaactt acgagcagat caaagctgcc gttaaagctg ctgctgaagg
    18721 cgaaatgaaa ggcgttctgg gctacaccga agatgacgta gtatctaccg atttcaacgg
    18781 cgaagtttgc acttccgtgt tcgatgctaa agctggtatc gctctgaacg acaacttcgt
    18841 gaaactggta tcctggtacg acaacgaaac cggttactcc aacaaagttc tggacctgat
    18901 cgctcacatc tccaaataag ttgagatgac actgtgatct aaaaagagcg acttcggtcg
    18961 ctcttttttt tacctgataa aatgaagtta aaggactgcg tcatgattaa gaaaattttt
    19021 gcccttccgg tcatcgaaca aatctcccct gtcctctccc gtcgtaaact ggatgaactg
    19081 gacctcattg tggtcgatca tccccaggta aaagcctctt ttgcattaca gggcgcacac
    19141 cttctctcgt ggaaacctgc gggtgaagaa gaagttctgt ggttgagcaa caacacaccg
    19201 ttcaaaaatg gcgtcgctat tcgcggtggc gtaccggttt gctggccgtg gtttggtccg
    19261 gcggcacaac aaggtctgcc tgcgcacggt tttgcccgca acctgccgtg gacgctgaaa
    19321 tcacatcgtg aagatgctga tggcgtagcg ctgacttttg aattgacaca aagcgaagag
    19381 acgaaaaaat tctggccgca cgactttacg ctgttagcgc atttccgcgt gggtaaaacc
    19441 tgtgaaatcg atcttgaatc acatggcgaa tttgaaacca cctctgccct gcatacctac
    19501 tttaacgtgg gtgatatcgc taaggtaagc gtcagtgggc tgggcgatcg cttcattgat
    19561 aaagtaaatg acgcgaaaga agatgtactg accgacggta ttcagacctt ccctgaccgt
    19621 accgatcgcg tgtatctgaa tccgcaagat tgcagcgtga ttaatgatga agcgctgaat
    19681 cgtattatcg ccgtaggcca ccagcatcat ctgaacgttg tcggctggaa cccgggaccg
    19741 gcgctttcag ttagcatggg cgacatgccg gatgatggct acaaaacatt tgtttgtgta
    19801 gaaacggctt acgcttcaga aacgcaaaaa gtgaccaaag agaaacctgc acatctggcg
    19861 caatccattc gcgttgcgaa acgttaattt gcgttaatgt tgtgtgccgg gtgcgatgca
    19921 tccggcacgt atcaacatca caccatatcc agcgcagttt ttccttttgg tgccggatac
    19981 gctttatcca gcatggttaa ttccgctgac gaaagttcga cctcaagcac agccgcattc
    20041 tgttggacat gggcaatcgt ggccgctttt ggaatcgcca tcacaccctg atggctgatt
    20101 acccacgcca acaatacttg tgccgcgctg atattgtgag catgtgcaat ttcgttgact
    20161 accgcgtttt ttaacagtcc attgcgcaac cgcccggcct gggctaatgg actgtaagcc
    20221 atcaccggca tctgctgttg ctggcaccag gggagtagat cgtactcaat ccctcgtgaa
    20281 ccgagatggt aaagcacctg attagtggca cactgatttc cccccggcag ctgccagagt
    20341 tcctgcatat cagcatgatc aaggttagaa acgccccagc ggcggatttt tccctgggcg
    20401 atcaattttt ccatcgctgc cacagtctct tcaaaagcga aactgccaga ccagtgtaat
    20461 aagtagagat cgagataatc agtattgaga cggcgtaaac tggcttcgca tgcatttatc
    20521 gctttttgcc cgccagcatt ccacggatag actttagaga cgagaaagac gttatcgcgc
    20581 agaccggtta atgcttcccc aaccaccttt tcggcaccgc catcggcata catttcggcg
    20641 gtatcaatga gggttaaacc gagttcaatg cccgcgcgta gtgcagcaac ttctgttttg
    20701 cgctgactgg catcttcgcc aatataccat gttccctgcc ctatggctgg cagtgagaca
    20761 tcgccactaa attgaatcat tttttgttgc attgtttcct cccaggtatt gcaccaccgt
    20821 aatgcaaaac agggcgtgac gccctgtttt tatgcacaaa atgccctgga aagatgcatt
    20881 atcagaattt gtaggtgatc ccggtagaaa tcaggccagt ccaggattta tccaccatcg
    20941 ggctgtcagt aacttcatca gacagacggg tatagcgcgc ggtaccgtaa acactccagt
    21001 cgccgaggaa gttgtagctg gcgctcagct ccaggtaagg gctccagctg tcgttcgggt
    21061 tatagccacg cagaccgctg cgagcggact ctttgcgcga tacgccataa tagtattcgt
    21121 tctggttttc gctgttccac tgcacaccaa tacccggagt cacggtcagg ccaccgttgg
    21181 tgtaacgata caaccaggcc atatcccaga caatgccgtt gctgttatcc agggtatcgc
    21241 cagccagggt ggtacgcagg taaccgtact gggtaaagtg agcataagac agaccagcca
    21301 tcatggtgct cttacggtca tccaggtgac gcatttggtg atcgccactg tctttcgctt
    21361 tgaagtaaag cggcgaccag taagcggtaa ttgaaagttt atccgttgcg tcattccaca
    21421 ggtagtaacc accacctaag ccacggaacc agaagttatc gccttcatag ttgattaccg
    21481 gtactgggta aacatcggta tcgtaatctt tatatgggtg ctcaacgaca cctacgcctg
    21541 cgcccaggga aaatttacct tcagcgtgcg ctacgcctgc agacgttgcg ataagcactc
    21601 caagtgccag aagtttgagt ttggtcacaa ttaatcattc cttaaacaaa tgtttagcgg
    21661 gcgacaaagt ttacccgtca atacatcgaa acccaacctt tttacgtttt cattttttaa
    21721 agtaactgtt taattttcct gacgcggatg acaccgcgct tacagccaaa tgaatttagc
    21781 gttactggct accctggtct ttacattaat tatgcaaaat ttatggatga gttgttgata
    21841 tgccattgaa attaagaaag ccgtgcaggc aagttttcca tttgccatct acgcttaatt
    21901 ttgaaggtgt atcaccgggc acgttgttct catcgtcgat aaaatggcat gagagttgct
    21961 gtgttttagc aagagacgtc gttcagttta cctcttccgg gagcctctac tattcatatg
    22021 aacggctctt aacctgtgct aaaaaacgaa aggacggcat accatgaata tattcgatca
    22081 ctatcgccag cgatatgaag ctgccaagga cgaagagttc acactgcagg agtttcttac
    22141 cacttgtcgg caagatcgca gtgcttatgc caacgcggct gagcggctat tgatggctat
    22201 cggtgagcct gtcatggtcg atacagccca ggaacccaga ctttctcgac tcttttctaa
    22261 ccgggtcatt gcacgttatc cggcatttga agagttttac ggcatggaag acgcgattga
    22321 acagattgtc tcttatctga aacacgcggc tcaggggctg gaagagaaga aacaaatcct
    22381 gtatctgctg gggcctgtgg gtgggggtaa atcatcgctt gctgagcgac tgaaatcatt
    22441 aatgcagctc gtaccgattt atgtattgag cgccaacggt gagcgtagcc cggtcaacga
    22501 tcatccgttc tgtcttttca atccgcagga agatgctcag attctggaaa aagagtatgg
    22561 cattcctcgc cgttatctcg gcaccatcat gtcgccatgg gcggcaaaac gcctgcatga
    22621 atttggtggc gatatcacta agttccgggt agtgaaggtc tggccgtcaa ttctgcaaca
    22681 aattgctatc gccaaaacgg aacccggtga tgagaacaac caggacatct ccgcgctggt
    22741 tgggaaagtc gatattcgta aactcgaaca ctacgcgcag aatgacccgg acgcctacgg
    22801 ctattccggt gcgctgtgcc gcgccaacca agggatcatg gaatttgtcg agatgtttaa
    22861 agcaccgata aaagtgctgc atccgctgtt aaccgccact caggaaggta actacaacgg
    22921 gacggaaggt atctccgccc tgccgttcaa cgggattatt ctcgcccact cgaacgaatc
    22981 cgaatgggtc actttccgta ataacaaaaa caacgaagcc ttcctcgacc gtgtttacat
    23041 cgtgaaggtg ccgtattgct tacgcatttc cgaagagatc aaaatctacg agaaattgct
    23101 taatcacagt gaattgactc acgtcccctg cgcccctggc acgctggaaa cgctgtcacg
    23161 tttttccatt ctttcgcgcc ttaaagagcc agaaaactcc agcatttatt caaagatgcg
    23221 ggtttatgat ggcgaaagtc tgaaagacac cgatcccaaa gccaagtcgt atcaggaata
    23281 tcgtgactac gccggtgtcg atgaagggat gaacggtctg tcgacgcgtt ttgcgtttaa
    23341 gatcctctcc cgcgtgttca acttcgatca tgtagaagtg gcggcaaacc cggtccatct
    23401 gttctacgtc ctggaacagc agatcgagcg cgagcagttc ccacaagagc aggcagaacg
    23461 ctatctggag ttcctgaaag gttatctgat cccgaaatat gccgagttta tcggcaaaga
    23521 gatccagacg gcctaccttg aatcctattc cgaatatggg caaaacattt tcgaccgtta
    23581 tgttacctac gcggatttct ggattcagga tcaggagtat cgcgatccgg ataccgggca
    23641 gctgtttgac cgcgagtctc ttaacgccga gctggagaaa atcgagaaac cggcggggat
    23701 cagtaatcca aaagatttcc gcaacgagat tgttaacttc gtactgcgcg ccagagcgaa
    23761 taacagcgga cgcaatccga actggaccag ctatgaaaaa ctgcgcacgg ttatcgagaa
    23821 gaaaatgttc tccaataccg aggagctgtt gccggttatc tcgtttaacg ccaaaacgtc
    23881 aaccgacgag cagaagaaac acgacgactt tgtcgaccgt atgatggaaa aaggctacac
    23941 ccgtaaacag gtgcgtttac tgtgcgaatg gtatttgcgc gtacgtaaat cgtcttaaca
    24001 accctggccc ggtacacatg ttaccgggcc tacaacgaca gcgaaccgtg ggcctgagaa
    24061 gcggcaacac aggcgtagca tacagttggc aaatgtagta cggggggcat atgacctggt
    24121 ttattgaccg gcgtctgaac ggcaaaaaca aaagcatggt gaatcgccag cgttttttac
    24181 gccgttataa agcgcaaatt aaacagtcga tctccgaggc cattaataag cgttcggtga
    24241 ctgacgtcga cagcggcgaa tccgtatcca ttcccacgga agatattagc gaaccgatgt
    24301 ttcatcaggg gcgtggcggt ctgcgccacc gcgtgcatcc gggcaatgac catttcgtcc
    24361 agaacgaccg aattgaacgt ccccagggtg gcggcggagg ttccggtagt ggtcagggcc
    24421 aggccagcca ggatggtgaa ggtcaggatg aatttgtctt tcagatttcg aaagatgagt
    24481 atcttgattt gctctttgaa gatttggcct taccgaatct gaaacaaaac caacaacgcc
    24541 agctgaccga atataaaacg catcgggcgg gttataccgc taacggcgtt ccggcaaata
    24601 tcagcgttgt gcgctccttg cagaactcac tggcgcgacg cacagccatg acggcaggca
    24661 agcggcggga acttcatgca ctggaagaga atttggccat catcagcaac agtgaacctg
    24721 cgcagctgct ggaagaggaa cgtctgcgca aagaaattgc agaattacgt gccaaaattg
    24781 aacgcgtccc ttttattgat accttcgatt tacgttacaa gaactacgag aagcggcccg
    24841 atccctccag ccaggcagtg atgttttgcc tgatggacgt ttccggttca atggatcaat
    24901 ccactaaaga tatggctaag cgtttttata ttctgctgta tctgttcctc agcagaacgt
    24961 ataagaacgt ggaagtcgta tacatccgcc atcataccca ggcgaaagaa gtcgatgaac
    25021 atgagttttt ctactcgcag gaaacaggcg gcaccattgt ttccagcgcc ctgaaactga
    25081 tggatgaggt agtgaaagag cgttataacc cggcacagtg gaatatttac gctgcacaag
    25141 catcggacgg cgataactgg gccgatgact ctccgctttg ccatgaaatc ctggcgaaaa
    25201 aattattacc tgttgttcgt tattacagct atatcgaaat tacccgtcgt gcacatcaga
    25261 cattgtggcg agaatatgag catctgcaat ctactttcga caactttgcg atgcagcaca
    25321 tccgcgacca ggatgatatt tatccggtgt tccgtgaact gtttcataaa caaaattcaa
    25381 cagctaaaga ctaaaactat cagccaggtc attatcgcct ggctgatttt tagcttactg
    25441 taaattatca catttattac ataca
//
LOCUS       DBANUU010000065        25217 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000065 DBANUU010000000
VERSION     DBANUU010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.065"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..759
                     /locus_tag="ACIE68_002585"
     CDS             <1..759
                     /locus_tag="ACIE68_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFX5908885.1"
                     /translation="GNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDI
                     VWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAIN
                     FSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEG
                     IPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMD
                     FAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            915..2450
                     /gene="gadC"
                     /locus_tag="ACIE68_002586"
     CDS             915..2450
                     /gene="gadC"
                     /locus_tag="ACIE68_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFX5908886.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2581..3900
                     /locus_tag="ACIE68_002587"
     CDS             2581..3900
                     /locus_tag="ACIE68_002587"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFX5908887.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4277..5659
                     /gene="dosC"
                     /locus_tag="ACIE68_002588"
     CDS             4277..5659
                     /gene="dosC"
                     /locus_tag="ACIE68_002588"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFX5908888.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5684..8082
                     /gene="dosP"
                     /locus_tag="ACIE68_002589"
                     /gene_synonym="pdeO"
                     /pseudo
     CDS             5684..8082
                     /gene="dosP"
                     /locus_tag="ACIE68_002589"
                     /gene_synonym="pdeO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
     gene            8340..8921
                     /gene="ddpX"
                     /locus_tag="ACIE68_002590"
     CDS             8340..8921
                     /gene="ddpX"
                     /locus_tag="ACIE68_002590"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFX5908889.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQVCPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHDRSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            8935..10485
                     /locus_tag="ACIE68_002591"
     CDS             8935..10485
                     /locus_tag="ACIE68_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFX5908890.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTSVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYS
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10487..11509
                     /locus_tag="ACIE68_002592"
     CDS             10487..11509
                     /locus_tag="ACIE68_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFX5908891.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQHLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFSATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLSGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11506..12402
                     /gene="ddpC"
                     /locus_tag="ACIE68_002593"
     CDS             11506..12402
                     /gene="ddpC"
                     /locus_tag="ACIE68_002593"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFX5908892.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12399..13385
                     /locus_tag="ACIE68_002594"
     CDS             12399..13385
                     /locus_tag="ACIE68_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFX5908893.1"
                     /translation="MTQPVLDIQQLHLSFPGFHGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIDLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            13378..14304
                     /locus_tag="ACIE68_002595"
     CDS             13378..14304
                     /locus_tag="ACIE68_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFX5908894.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRV
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(14360..14791)
                     /gene="osmC"
                     /locus_tag="ACIE68_002596"
     CDS             complement(14360..14791)
                     /gene="osmC"
                     /locus_tag="ACIE68_002596"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFX5908895.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            15136..15351
                     /gene="bdm"
                     /locus_tag="ACIE68_002597"
     CDS             15136..15351
                     /gene="bdm"
                     /locus_tag="ACIE68_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFX5908896.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAQHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15453..15590
                     /gene="sra"
                     /locus_tag="ACIE68_002598"
     CDS             15453..15590
                     /gene="sra"
                     /locus_tag="ACIE68_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFX5908897.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15747..17444
                     /gene="maeA"
                     /locus_tag="ACIE68_002599"
     CDS             15747..17444
                     /gene="maeA"
                     /locus_tag="ACIE68_002599"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFX5908898.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17578..18588
                     /gene="adhP"
                     /locus_tag="ACIE68_002600"
     CDS             17578..18588
                     /gene="adhP"
                     /locus_tag="ACIE68_002600"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFX5908899.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVCTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            18734..19018
                     /locus_tag="ACIE68_002601"
     CDS             18734..19018
                     /locus_tag="ACIE68_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFX5908900.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(19425..20078)
                     /gene="fdnI"
                     /locus_tag="ACIE68_002602"
     CDS             complement(19425..20078)
                     /gene="fdnI"
                     /locus_tag="ACIE68_002602"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFX5908901.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(20071..20955)
                     /gene="fdnH"
                     /locus_tag="ACIE68_002603"
     CDS             complement(20071..20955)
                     /gene="fdnH"
                     /locus_tag="ACIE68_002603"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFX5908902.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20968..24015)
                     /gene="fdnG"
                     /locus_tag="ACIE68_002604"
     CDS             complement(20968..24015)
                     /gene="fdnG"
                     /locus_tag="ACIE68_002604"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(23428..23430),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFX5908903.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQSEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYNANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            24247..25128
                     /gene="yddG"
                     /locus_tag="ACIE68_002605"
     CDS             24247..25128
                     /gene="yddG"
                     /locus_tag="ACIE68_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFX5908904.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGATAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         6147 a         6408 c         6403 g         6259 t
ORIGIN      
        1 ggtaactatg agttcccaca accgctgcac gatgcgctgg ataaattcca ggccgatacc
       61 ggtatcgaca tcgacatgca catcgacgct gccagcggtg gcttcctggc accgttcgtc
      121 gccccggata tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag tgcttcaggc
      181 cataaattcg gtctggctcc gctgggctgc ggctgggtta tctggcgtga cgaagaagcg
      241 ctgccgcagg aactggtgtt caacgttgac tacctgggtg gtcaaattgg tactttcgcc
      301 atcaacttct cccgcccggc gggtcaggta attgcacagt actatgaatt cctgcgcctc
      361 ggtcgtgaag gctacaccaa agtacagaac gcctcttacc aggttgccgc ttatctggcg
      421 gatgaaatcg ccaaactggg gccgtatgag ttcatctgta ccggtcgccc ggacgaaggc
      481 attccggcgg tttgcttcaa actcaaagat ggtgaagatc cgggatacac cctgtatgac
      541 ctctctgaac gtctgcgtct gcgcggctgg caggttccgg ccttcactct cggcggtgaa
      601 gccaccgaca tcgtggtgat gcgcattatg tgtcgtcgcg gcttcgaaat ggactttgct
      661 gaactgttgc tggaagacta caaagcctcc ctgaaatatc tcagcgatca cccgaaactg
      721 cagggtattg cccagcagaa cagctttaaa catacctgat aacgtttaac ggtaacggtg
      781 tcccgaaacg aacccgtttc gggacaattt ccaaagtctg ttcactggca ttagcaacgg
      841 aaaatattgt tctgaatacg cttcagaaca aaacaggtgc ggttccgaca ggaataccgt
      901 tttaggggga taatatggct acatcagtac agacaggtaa agctaagcag ctcacattac
      961 tcggattctt tgccataacg gcatcgatgg taatggctgt ttatgaatac cctaccttcg
     1021 caacatcggg cttttcatta gtcttcttcc tgctattagg cgggatttta tggtttattc
     1081 ccgtgggact ttgtgctgcg gaaatggcca ccgtcgacgg ctgggaagaa ggtggtgtct
     1141 tcgcctgggt atcaaatact ctggggccga gatggggatt tgcagcgatc tcatttggct
     1201 atctgcaaat cgccattggt tttattccga tgctctattt cgtgttaggg gcactctcct
     1261 acatcctgaa atggccagcg ctgaatgaag accccattac caaaactatt gcagcactca
     1321 tcattctttg ggcgctggca ttaacgcagt ttggtggcac gaaatacacg gcgcgaattg
     1381 ctaaagttgg cttcttcgcc ggtatcctgt tacctgcatt tattttgatc gcattagcgg
     1441 ctatttatct gcactccggt gcccccgttg ctatcgaaat ggattcgaag accttcttcc
     1501 ctgacttctc taaagtgggc acactggttg tatttgttgc cttcattttg agttatatgg
     1561 gcgtagaagc ttccgcaact cacgtcaatg aaatgagcaa ccccgggcgc gactatccac
     1621 tggctatgtt actgctgatg gtggcggcaa tctgcttaag ctctgttggc ggtttatcta
     1681 ttgcgatggt cattccgggt aatgaaatca acctctccgc aggggtaatg caaaccttta
     1741 ccgttctgat gtcccatgtg gcaccggaaa ttgagtggac ggttcgcgtg atctccgcac
     1801 tgctgttgct gggtgttctg gcggaaatcg cctcctggat tgttggtcct tctcgcggga
     1861 tgtatgtcac agcgcagaaa aacctgctgc cagcggcatt cgctaaaatg aacaaaaatg
     1921 gcgtaccggt aacgctggtc atttcgcagc tggtgattac ttctatcgcg ttgatcatcc
     1981 tcaccaatac cggtggcggt aacaacatgt ccttcctgat cgcactggcg ctgacggtgg
     2041 tgatttatct gtgtgcttat ttcatgctgt ttattggcta cattgtgttg gttcttaaac
     2101 atcctgactt aaaacgcaca tttaatatcc ctggtggtaa aggggtgaaa ttggtcgtgg
     2161 caattgtcgg tctgctgact tcaattatgg cgtttattgt ttccttcctg ccgccggata
     2221 acatccaggg tgattctacc gatatgtatg ttgaattact ggttgttagt ttcctggtgg
     2281 tacttgccct gccctttatt ctctatgctg ttcatgatcg taaaggcaaa gcgaataccg
     2341 gcgtcactct ggagccaatc aacagtcaga acgcaccaaa aggtcacttc ttcctgcacc
     2401 cgcgtgcacg ttcaccacac tatattgtga tgaatgacaa gaaacactaa ccatgtctga
     2461 atggttataa gacaagggag cgataagtca tcgctccctt tttcgtgctt gctgtcgtat
     2521 tgacttaacc gggataagta cgagaatgag cgcacatctg tttaccggaa accagcacat
     2581 atggatatct gctcccgaaa caagaaatta acgattagaa gaccagcgat actagttgca
     2641 ctggcacttt tactgtgtag ttgtaaaagc acgcctccag agtccatggt gacaccacca
     2701 gcaggttcaa agccaccagc cacgacgcaa caatcgtcac aaccgatgcg tggcatctgg
     2761 ctggccacgg tttctcggct cgactggcca ccggtttcct cggttaacat tagtaacccc
     2821 accagccggg cccgtgtaca acaacaggcg atgatcgaca aactggatca tctgcaacgt
     2881 ctcggcataa acacggtctt tttccaggtc aagccggacg gtaccgccct gtggccatcg
     2941 aaaattttgc cgtggtccga tcttatgacc ggtaagattg gtgaaaatcc gggttacgat
     3001 ccgctgcaat ttatgctcga cgaagcccac aagcgtggga tgaaagtaca cgcctggttt
     3061 aacccctatc gcgtatcggt taatacgaag cccggtacta tcagggaact gaatagcact
     3121 ctgtctcaac aaccggcgag cgtctatgtg caacaccgcg actggatcag aacgtctggc
     3181 gatcgctttg tcctcgaccc gggcatccct gaggttcagg actggatcac atcaatagtc
     3241 gcagaagtgg tttcccgcta tcccgtagat ggcgtgcagt ttgacgacta tttctatacg
     3301 gagtcaccgg gttcacggct aaatgataac gaaacgtacc gtaaatacgg aggcgcattt
     3361 gcgtcaaaag cagactggcg gcgcaacaat actcagcagt taattgcaaa ggtatcgcac
     3421 accattaaaa gcattaagcc gggagtcgaa tttggtgtta gcccggcagg cgtgtggcgt
     3481 aaccgatcac acgatccgct cggttccgat acccgaggcg cggcagccta tgacgaatcc
     3541 tacgctgaca cccgtcgatg ggtggaacaa ggattgctgg attacattgc tccccaaatt
     3601 tactggccgt tctcacggag tgccgcgcgt tatgacgtgt tggcaaaatg gtgggcggat
     3661 gtcgttaaac cgaccaggac ccgcctgtat atcggtatcg ccttctataa agtgggtgaa
     3721 ccctcaaaga tagagccaga ctggatgatt aacggcggcg taccggaact gaaaaagcag
     3781 ctcgatctta acgatgcggt accagaaatt agcggcacca tcttgttccg tgaggactat
     3841 ctgaataaac cgcagactca acaagcggtc agctatctgc aaagtcgttg gggcagttaa
     3901 actcccattc ttactttgta acaagccggt gctttaccca ccggcttgtt accctcttga
     3961 actatcccac tactaatcat gcttactaaa gtcaaattaa ccacgcaatt aaccacactt
     4021 agttaaacag ccataaatcc cctaataaaa atgtatgaat atgaaaaaac atacgatatt
     4081 cgctgtcacc gaaaaataat tacgatgggc tatactcttc tgaagaacag tagcgtagat
     4141 taaaactgtc ctaagtcgct tatttctttt cagtatatct tcatatttca ggagaatatg
     4201 gtgcagttag tcgatctcgc acgttgcgtt tcgttttgat tccgtctgat ttcccagcct
     4261 tataagggtg tgaattatgg agatgtattt taaaagaatg aaagatgagt ggaccggact
     4321 tgtcgaacag gcagatcctc tcattcgtgc taaagccgca gaaattgccg ttgcgcatgc
     4381 tcattatctg agtatcgagt tttatcgaat tgtccgcatc gacccgcatg ccgaagaatt
     4441 cttgagtaat gaacaagttg agcggcagtt gaaaagtgcg atggaacgct ggattattaa
     4501 cgtgctttct gcccaggttg acgatgtcga aaggctaata caaatccagc ataccgtcgc
     4561 ggaagtgcat gcccgcatag gaattccggt agaaattgtc gagatggggt ttcgggtgct
     4621 gaaaaaaatc ctctatccgg taatcttctc ttcggattat tccgccgcag aaaaacttca
     4681 ggtctatcat ttctcgatta acagtattga tatcgcgatg gaagtgatga cccgcgcgtt
     4741 tacctttagt gacagtagtg cctcaaagga agatgaaaac tatcgtatct tctcgttgct
     4801 ggaaaacgcc gaagaagaaa aagaacggca aatagcctca atactttcat gggaaataga
     4861 tattatctat aaaatcctgc tggattctga tttaggcagt agtttgcctt taagccaggc
     4921 tgattttggc ctgtggttta accataaagg tcgacattat tttagtggca ttgccgaagt
     4981 aggccatatc tcccgtctga ttcaggattt cgacggtata ttcaatcaaa ccatgcgtaa
     5041 caccaggaat ctgaataaca gaagtctgcg ggtgaaattt ttattacaga taagaaatac
     5101 tgtatcgcaa attattacct tgctgcgtga attgtttgaa gaagtatcgc gccacgaagt
     5161 cggtatggat gtactgacga aattacttaa ccgccgtttc ctgccgacta tcttcaaacg
     5221 cgaaattgcc catgccaacc ggaccggtac accgctgtca gtgctgatta ttgacgttga
     5281 taaattcaaa gagatcaacg atacgtgggg ccataacact ggcgatgaaa ttctgcgtaa
     5341 agtctctcag gccttttatg acaacgtccg cagtagtgat tatgttttcc gctacggggg
     5401 cgatgaattt atcattgttt tgactgaagc ttcggaaaac gaaacgttac gtaccgcaga
     5461 acgtattcgc agtcgggtgg agaaaaccaa actgaaagcc gcaaacggcg aggatattgc
     5521 cctctcactt tccatcggtg ccgccatgtt taatggtcat cctgactatg agcgcctcat
     5581 tcaaatagcc gatgaagctc tgtatatcgc caaaagacga ggtagaaacc gtgttgaact
     5641 ctggaaagcc agtctttaga tgcgccagga tgcagaggta atcatgaagc taaccgatgc
     5701 ggaaaatgcc gccgatggca tttttttccc cgcccttgag caaaatatga tgggtgcggt
     5761 gttaattaac gaaaatgatg aagtgatgtt tttcaacccc gccgcagaga agctctgggg
     5821 gtacaaacgt gaagaagtca ttggcaataa cattgatatg ctgattccgc gggatttgcg
     5881 tcctgcgcat cctgaataca ttcgtcataa ccgtgaaggc ggtaaagcgc gcgtcgaggg
     5941 gatgagtcgg gagctgcagc tggagaaaaa agacggcagt aaaatctgga cccgttttgc
     6001 gctatcgaaa gtgagcgccg aggggaaagt ttattacctg gcgctggtac gggatgccag
     6061 cgtagaaatg gcgcaaaaag aacagacccg acaattgatt attgccgttg accatctcga
     6121 ccgaccggtg attgtcctcg atccggaacg ccatattgtg cagtgcaatc gcgcatttac
     6181 cgaaatgttt ggttactgca ttagcgaagc cagcggtatg cagcccgata cactcctgaa
     6241 cattcctgaa ttccctgccg ataaccgcat tcgtttacaa cagttgctat ggaaaaccgc
     6301 ccgcgatcag gacgaatttc tgctgttgac gcgcaccggt gaaaaaatct ggattaaagc
     6361 ctctatcagc ccggtttatg acgtgctcgc gcatctgcag aacctggtaa tgactttctc
     6421 ggatatcacc gaagaacggc agattcgcca gcttgaaggc aatattctcg ccgccatgtg
     6481 cagcagcccg ccatttcatg aaatggggga aatcatttgt cgtaacatcg aatctgtact
     6541 caacgaatcg catgtttcgc tgttcgcact gcgcaacggg atgccgatac actgggcgtc
     6601 atcttcgcac ggtgcagaag ttcaaaatgc gcaaagctgg tcagcgacca ttcgtcagcg
     6661 tgatggcgcg cctgcgggga tcctgcaaat taaaacctcg tcaggagcag aaaccagcgc
     6721 ctttatcgaa cgcgtggcag atatcagcca gcatatggcc gcgctggcgc tggaacagga
     6781 aaaaagccgt cagcatattg aacaactcat ccaatttgat ccgatgaccg gtctgccaaa
     6841 tcgcaataac ctgcacaatt acctcgatga cctggtcgac aaagccgtct ctccggtggt
     6901 gtatctcatc ggtgttgacc atattcaggt gtgattgata gccttggcta tgcgtgggcc
     6961 gatcaggcat tgctggaagt ggtcaatcgc tttcgtgaaa aactcaaacc ggatcagtat
     7021 ctctgtcgta tcgaaggtac gcagtttgtc ctcgtgagcc tggaaaacga cgtcagtaac
     7081 attacccaga tcgccgatga gctacggaat gtggtcagca agccgataat gattgacgat
     7141 aaacccttcc cgcttacctt gagtattggc atcagctacg acgtggggaa aaaccgcgat
     7201 tacctgctct ccactgccca taacgcgatg gattatattc gcaagaatgg cggtaacggc
     7261 tggcagttct tcagcccggc gatgaacgaa atggtaaaag agcgtttggt tttaggcgca
     7321 gcgctgaaag aaacgattag caataaccaa ctgaaactgg tttaccagcc gcaaatcttc
     7381 gcagaaacgg gtgaactgta cggcatcgaa gcccttgctc gctggcacga tccccagcat
     7441 ggtcatgtac ccccttcacg gtttattcct ctcgcagaag agattggtga aatcgaaaat
     7501 attgggcgct gggtcatcgc ggaagcttgc cgtcagttag cagaatggcg tagccagaat
     7561 attcatatcc cggcgttatc cgtgaacttg tcggcgctgc actttcgcag taatcaactg
     7621 cctaatcagg tgtctgatgc aatgcaagcc tggggtattg acggccacca gctgacggtg
     7681 gaaatcacgg aaagcatgat gatggaacac gataccgaaa tctttaagcg cattcagatc
     7741 ctgcgtgata tgggcgtggg tttatcggta gatgattttg gcacgggctt ttccggatta
     7801 tcccgcttag tcagtcttcc ggtaacggaa atcaaaattg acaaaagttt tgtcgatcgt
     7861 tgtctgacgg aaaaacgcat ccttgcctta cttgaagcca ttaccagcat tgggcaaagc
     7921 ctcaatttaa ccgtcgtggc ggaaggcgtc gaaaccaaag agcaatttga gatgctacgc
     7981 aagatccact gtcgcgttat tcagggatat ttcttttccc gccccctgcc cgccgaagaa
     8041 attccaggct ggatgagcag cgtgttaccg ctgaaaatct gacaaattcc tctcgcccgc
     8101 actcgcgggt attcatttaa cgtgacactg tcacttaaat actgtgattt cagctacgat
     8161 tccgggagat tccttcctta acttcccctc ttgcaccaaa actgcactac gctgcacaaa
     8221 tgttgatcaa aagttaaaaa atataacttt aagccagcat tctggcgctt ctcccggcat
     8281 ggcatgagat ctgcataagc ggaaagcgca gcaatttttg tcttatacag gaaccgttta
     8341 tgtcggatac caccgaactg gttgatttag ccgtaatctt ccctgatctg gagatcgaat
     8401 tgaaatacgc ctgcgctgat aacatcacag gtaaagctat ttatcagcaa gcgcgttgtc
     8461 tgttacacaa ggatgcgatt accgcgctgg cgaaaagtat cagtatcgcc cagctgtcag
     8521 gcttacaact ggtgatttac gatgcgtatc gcccacaaca agcacaggcg atgttgtggc
     8581 aagtctgccc agacccgcaa tatgttgttg atgtgacggt cggttctaat cacagccgtg
     8641 gcacggcgat cgacctgacg cttcgtgatg agcacgggaa catcctcgat atgggcgcag
     8701 ggttcgatga aatgcacgat cgctcccatg cttaccatcc ttccgtcccg cccgccgctc
     8761 agcgcaatcg cctgttgctg aatgcgatta tgactggtgg cggttttgtc ggtatctcca
     8821 gcgaatggtg gcacttcgaa ttaccccagg cagcgagtta cccactgctc gctgatcaat
     8881 tcacctgttt tatatcccct ggcacacagc acgtcagtta atcaggaaac tgtcatgaaa
     8941 agatcgatat cgtttcgtcc tacattgctc gcgctcgtcc ttgccacaac tttcccggtt
     9001 gcgcacgccg ccgtaccgaa agatatgctg gtgatcggta aggctgccga tccacaaacc
     9061 ctcgacccgg cggtgacaat tgataataac gactggacgg tgacctaccc gtcttatcag
     9121 cggctggttc agtacaaaac ggacggtgat aaaggctcaa ccgacgttga aggcgatctg
     9181 gcaagtagct ggaaagcgtc tgacgatcaa aaagagtgga cgttcaccct gaaggataat
     9241 gctaaatttg ccgatggcac atctgtcact gccgaagcag taaaactctc ttttgagcgg
     9301 ttactaaaaa tcggccaggg gccagcagaa gcatttccca aagatttaaa gattgatgct
     9361 cccgacgaac atacggtgaa gtttaccctt agccaaccat tcgcaccgtt cctctacacg
     9421 ctggcgaatg acggtgcatc cattatcaat ccggcggtgt taaaggaaca tgcagcggat
     9481 gatgctcgcg gcttcctcgc gcaaaatacc gccggttccg gaccatttat gctgaaaagc
     9541 tggcaaaaag gtcagcaatt agttctggtg ccaaatccgc attactccgg caataaaccg
     9601 aactttaagc gagtatcggt aaaaattatc ggtgaaagtg cctcccgtcg cctgcagctc
     9661 tcccgtggcg acattgacat tgccgatgcg ctgccggtgg atcaactcaa cgccctgaag
     9721 caggaaaata aagtcaatgt ggcagagtat ccgtcactgc gcgtcaccta tctgtatctg
     9781 aataacagca aagcgccact taatcaggcg gatctgcgtc gggccatttc ctggtctacc
     9841 gattatcagg gcatggttaa cggcattctg agtggtaacg gaaaacaaat gcgcggcccg
     9901 attccggaag gcatgtgggg ctacgatgcg acggcaatgc aatacaacca tgacgaaacg
     9961 aaagccaaag ccgaatggga taaagtgacg agcaaaccca ccagcctgac gtttctctac
    10021 tccgataacg atccgaactg ggaaccgatc gctctggcga cacaatccag tctcaacaag
    10081 ctgggcatca atgtgaagct ggaaaagctg gcgaacgcca ccatgcgcga cagagtgggt
    10141 aaaggtgatt acgacattgc gattggcaac tggagtccgg attttgccga cccgtatatg
    10201 tttatgaatt actggtttga gtcagacaaa aaaggtctgc cgggtaaccg ctcgttctat
    10261 gaaaacagtg aggtcgataa gttactgcgc aatgcgcttg cgaccaccga ccagacgcag
    10321 cgtacccggg actaccagca ggcacagaaa atcgtcattg atgacgctgc ttatgtgtac
    10381 ctgttccaga aaaactacca actggcgatg aacaaagagg tgaaaggctt tgtgttcaat
    10441 cccatgctgg aacaggtctt caatatcaat accatgagta aataacgtga ccttctggag
    10501 tattttacgc caacgctgct gggggctggt gctcgtggtg gcgggcgtct gcgtgattac
    10561 gtttattatc tcgcatctga tccctggtga tccggcgcgg ttactggcgg gtgaccgcgc
    10621 cagcgatgct atcgtggaaa atattcgcca gcatctggga ctggaccagc cactgtacgt
    10681 acagttttac cgctacgtca gcgacctgtt tcatggtgac ctcggaacat ccattcgtac
    10741 cgggcgtccg gtgctggaag agttgcgtat atttttctcg gcgacgctgg aactggcttt
    10801 ttgtgccctg ctgctggcac tcctgattgg cattccgctg ggcatactct ctgccgtctg
    10861 gcgaaatcgc tggctggatc atctggtgcg aataatggcc attaccggaa tctccacacc
    10921 tgcgttctgg cttgggctgg gcgtcattgt gctgttttat ggtcatctgc aaattcttcc
    10981 cggcggcgga aggcttgatg actggctgga tccgccaacg cacgttaccg gcttttatct
    11041 gctcgatgcg ctgcttgaag gcaacggtga agtcttcttc aatgcgttgc aacatctcat
    11101 cttaccggca ttaacgctgg cgtttgttca cctggggatt gtcgcccgcc agatccgttc
    11161 agcgatgctg gaacaattga gtgaagacta cattcgtacc gccagggcca gcggcttgtc
    11221 cggctggtat atcgttttat gttatgcgct acccaatgcg ttgatcccat cgattaccgt
    11281 attgggtctg gcgctgggcg atttgttgta tggcgcagtg ctcaccgaaa ccgtttttgc
    11341 ctggcccgga atgggtgcat gggtagtgac atcaatacag gcgctcgact tcccggctgt
    11401 gatgggcttt gccgtcgtgg tttcatttgc ttatgtgctg gtcaacctgg tggtggattt
    11461 gctctatttg tggattgatc cgcgtatcgg acgtggaggt ggtgaatgat gctaagcgag
    11521 gaaacgtccg ccgtacgtcc acaaaaacaa acgcgattta atggtgcaaa actggtatgg
    11581 atgctgaaag gcagtccgct caccgtgacc ggcgcagtca tcattgtatt aatgctactg
    11641 atgatgattt tttcaccgtg gctggcgacg catgatccca acgccattga tttaaccgcc
    11701 cgccttttgc cgccttctgc ggcgcactgg tttggcaccg atgaagtggg acgcgatctg
    11761 ttcagccgcg tactggtcgg cagtcagcaa tcaattctcg ccggattagt ggtggtcgcc
    11821 attgcgggta tgattggttc gctactcgga tgtctatccg gtgtgcttgg cggacgcgca
    11881 gacgccatca tcatgcgcat catggacatt atgctgtcga ttccttcgtt ggtactgaca
    11941 atggcactgg ctgccgctct cgggccgagt ttgtttaacg ccatgctggc tattgctatt
    12001 gtgcgaattc ccttttatgt gcgcctggcg cgggggcaag cattagttgt acgccagtat
    12061 acctatgttc aggcggcgaa aacctttggc gcgtctcgtt ggcatctgat caactggcat
    12121 attttacgta actccctacc gccgctgatc gtgcaggcat cgctggatat cggtagcgcg
    12181 attttaatgg ccgccacgtt gggatttatt ggcctgggtg ctcaacaacc gagtgctgaa
    12241 tggggcgcga tggtagcaaa tggtcgcaac tatgtactcg atcagtggtg gtattgcgca
    12301 tttccggggg cagcgatttt gcttaccgcc gtcgggttta atctctttgg tgatggtatt
    12361 cgcgatctgc ttgacccgaa agcaggagga aagcagtcat gacccaaccc gttctggaca
    12421 ttcaacaact gcatttgagt ttccccggtt ttcacggtga tgttcacgcg ctcaacaatg
    12481 tgtccttgca gattaaccgc ggtgaaattg tcggtctggt gggagaatcc ggctcaggta
    12541 aatcagtcac cgcaatgctg attatgcgtc tgctaccgac gggcagttat tgcgtacatc
    12601 gggggcagat ttccctgctg ggagaagatg ttcttaacgc gcgggaaaaa cagcttcgtc
    12661 agtggcgcgg cgcacgagtg gcgatgatct ttcaggaacc gatgaccgcc ctcaatccga
    12721 cacgtcgaat aggtcttcag atgatggacg tgatccgcca tcatcaacca ataagtcgtc
    12781 gggaagccag agctaaagcg attgacctgc tggaagagat gcaaatcccg gatgccgtgg
    12841 aagttatgtc gcgctatccg tttgagcttt caggtggtat gcgccagcgg gtaatgattg
    12901 cgctggcatt ctcctgcgag ccgcaattga ttattgccga cgaaccgact acggcgctgg
    12961 acgtcacggt gcagttgcag gtactgcgtt tgcttaaaca taaagcccgc gccagtggaa
    13021 ctgcggttct gttcatcagc catgatatgg ccgtggtgtc gcaactgtgc gatagcgttt
    13081 acgtgatgta tgccggaagc gtaattgaaa gcggcgtgac ggcagacgtt atccatcatc
    13141 cccggcatcc gtataccatt ggtttgctgc aatgcgcacc agaacatgga gtaccacgcc
    13201 agctattacc cgccattcca gggacggtac caaacctcac ccatttgcct gacggctgcg
    13261 cttttcgcga tcgttgctat gcggcaggtg cacagtgtga aaacgtcccg gcgctgacag
    13321 cgtgtggtga caacaaccag cgctgcgcct gttggtatcc gcagcaggag gtcataagtg
    13381 tctgacacgt tattaacgtt acgcgacgtc catatcaatt tcccggcccg taaaaactgg
    13441 cttggtaaaa ctacggaaca tgttcatgcc attaatggta ttgatttaca gatccgccgt
    13501 ggtgaaacct tagggatcgt cggcgagtca ggctgcggca aaagcaccct cgcacagctt
    13561 ttaatgggta tgctgcaacc gagccacggg cagtacatcc gttctggctc acaacgcatt
    13621 atgcagatgg tgtttcagga cccgctctct tcgcttaatc cgcgcttacc ggtgtggcgc
    13681 gtcatcacag aaccgctctg gatagctaag cgtagtagtg aacaacagcg gcgagcgttg
    13741 gcagaggagt tggctgtgca ggtgggtatt cgtccggagt atctcgaccg cctgcctcat
    13801 gcgttctccg gcgggcagcg gcaacgtatc gccattgcca gagcactctc ttcgcagcct
    13861 gacgtgattg tgcttgatga gccaacctct gcgctggata tctccgttca ggcgcagatc
    13921 ctcaatttac tggtaacgct acaggaaaat cacgggctga cctatgtgct gatttcacac
    13981 aatgtctcgg tgatacgtca tatgagcgat cgggtggcgg tgatgtatct cgggcagatt
    14041 gtagaactgg gcgacgcgca gcaggtgctg acggcacctg cacatccata cacccgatta
    14101 ttgctggatt ccctccccgc cattgataaa ccgctggagg aagaatgggc attacgtaaa
    14161 acggatctgc cgggaaaccg cacgttgccg caaggctgtt ttttctacga acgttgcccg
    14221 ctggcaaccc acggatgtga agtccggcaa tcattagcaa taagggagga cggacgtgag
    14281 ctccggtgct ggcgggcgct gtagagatgc taacaacgca tcaggcattc acgcgcctga
    14341 cgcgtcatcc ggcaatgctt tacgatttca actggtaatc cagcgtaatt tccgctttca
    14401 gtacctgaga gaccgggcat cctgcttttg ctttctggat gatgccgtca aaggtagagg
    14461 catcaatacc cggcaccgca acttcactct tcagtgcgat tttcgtaatc gcaaaaccgg
    14521 catccacttt atccagcgac acatcggcgg tggtatcaat cgatgttggc gtgaatcccg
    14581 cttcccccag cattaatgaa agcgccattg agaaacatgc ggcatgcgct gcgccaatca
    14641 gttcttcagg gttggttcct ttttcgcctt caaaacgcgt gttaaatcca tacggctgtt
    14701 ggttcagcac gccactctcg gtggatactg ttcccttccc gcgtttgata tcgccttccc
    14761 agtgtgcctg acctttctta tggattgtca ttgttgctct cctgtgggct taaaaaggaa
    14821 attatagact acgtgagaaa cagcacgagg ataagcagga tattccgaat aaaatccggc
    14881 gaaaatatta cttctgcact taaattaaat tcccacaata gattaccggg aatatataac
    14941 ccaaaagata ttgtatcaat attaatcgga tcataccaat cacaatccct atgaatatca
    15001 tggtgttaaa atataaaaat catcaaccag gactaatctt aacaacgaag cggcaaatat
    15061 ttgccatgct gaatgtgctt tataaatctg cgatccgtag cagacaccat aaatacacag
    15121 acacggagaa tcactatgtt tacttattat caggcagaaa attcaacagc agaacccgct
    15181 ctggttaatg ccattgagca aggccttcgg gcacagcacg gtgttgtcac tgaagatgac
    15241 attctcatgg agctgaccaa atgggttgaa gcttctgata acgacatcct cagtgatatc
    15301 taccagcaaa cgatcaatta tgtggtcagt ggccagcacc ctacgcttta aggtgctatg
    15361 cttgatcggc aacctaattt aggggtttag cacgtgtttc ttcgctacgg cgatgttgtc
    15421 cttaaaacta gcaacaggat tgaggagtta aaatgaaatc gaaccgtcag gcacgtcata
    15481 ttcttggact ggaccataaa atttctaacc agcgcaaaat agttaccgaa ggtgacaaat
    15541 ccagcgtggt aaataaccca accggcagaa aacgccccgc tgaaaagtaa ttcataacca
    15601 tcagtcctca atgacgatta aacaccattg cctgcgcaat ggtgtttttg tttttatctg
    15661 ctttatactt ggggccgacg ccctggcggt aaagcaaaga cgataaaagc gtgccaggga
    15721 tggatattca aaaaagagtg agtgacatgg aaccaaaaac aaaaaaacag cgttcgcttt
    15781 atatccctta cgctggccct gtactgctgg aatttccgtt gttgaataaa ggcagtgcct
    15841 tcagcatgga agaacgccgt aacttcaacc tgctggggtt actgccggaa gtggtcgaaa
    15901 ccatcgaaga acaagcggaa cgagcatgga tccagtatca gggattcaaa accgaaatcg
    15961 acaaacacat ctacctgcgt aacatccagg acaccaacga aaccctcttc taccgtctgg
    16021 taaacaatca tcttgatgag atgatgcctg ttatttatac cccaaccgtc ggcgcagcct
    16081 gtgagcgttt ttctgagatc taccgccgtt cacgcggcgt gtttatctct taccagaacc
    16141 ggcacaatat ggacgatatt ctgcaaaacg tgccgaacca taatattaaa gtgattgtgg
    16201 tgactgacgg tgaacgtatt ctggggcttg gtgaccaggg catcggcggg atgggcattc
    16261 cgatcggtaa actgtcgctc tataccgcct gtggcggcat cagcccggcg tatacccttc
    16321 cggtggtgct ggatgtcgga acgaacaacc aacagctgct taacgatccg ctgtatatgg
    16381 gctggcgtaa tccgcgtatc actgacgatg aatactatga attcgttgat gaatttatcc
    16441 aggctgtgaa acaacgctgg ccggacgtgc tgttgcagtt tgaagacttt gctcaaaaaa
    16501 atgcgatgcc gttacttaac cgctatcgca atgaaatttg ttcttttaac gatgacattc
    16561 agggcactgc ggcagtaaca gtcggcacac tgatcgcagc cagccgcgcg gcaggtggtc
    16621 agttaagcga gaaaaaaatc gtcttccttg gcgcaggttc agcgggatgc ggcattgccg
    16681 aaatgatcat cgcccagacc cagcgcgaag gattaagcga ggaagcagcg cggcagaaag
    16741 tctttatggt cgatcgcttt ggcctgctga cggacaagat gccgaacctg ctacctttcc
    16801 agaccaaact ggtgcagaag cgcgaaaacc tcagtgactg ggataccgac agcgatgtgt
    16861 tgtcactgct ggatgtggtg cgcaatgtaa aaccagatat tctgattggc gtctcaggac
    16921 agaccgggct gtttacggaa gagatcattc gtgagatgca taaacactgt ccgcgtccga
    16981 tcgtgatgcc gctgtctaac ccgacgtcac gcgtggaagc cacaccgcag gacattatcg
    17041 cctggaccga aggtaacgcg ctggtcgcca ctggcagccc gtttaatcca gtggtatgga
    17101 aagataaaat ctaccctatc gcccagtgta acaacgcctt tattttcccg ggcatcggcc
    17161 tgggggttat tgcttccggc gcgtcacgta tcaccgatga gatgctgatg tcggcaagtg
    17221 aaacgcttgc tcagtattcg ccgctggtcc tgaacggcga aggtctggta ctaccggaac
    17281 tgaaagatat tcagaaagtc tcccgcgcaa ttgcgtttgc ggttggcaaa atggcgcagc
    17341 agcaaggcgt ggcggtgaaa acatctgccg aagctctgca acaggccatt gatgataatt
    17401 tctggcaagc cgaataccgc gactaccgcc gtacctccat ctaagcctgc gcccggtagt
    17461 gaaggctacc gggctatttc tctctccctt tttcagatct catccatact gggtagtggc
    17521 gaataaatct catttgcctc acctgctatg cagaacatca tccgaaaagg aggaactatg
    17581 aaggctgcag ttgttacgaa ggatcatcac gttgacgtta cggataaaac actgcgctca
    17641 ctgaaacatg gcgaagccct gctgaaaatg gagtgttgtg gtgtatgtca taccgatctt
    17701 catgttaaga atggcgattt tggtgacaaa actggcgtaa ttctgggcca tgaaggcatc
    17761 ggtgtggtgg cagaagttgg cccaggtgtc acctcattaa aaccaggcga tcgtgccagc
    17821 gtggcgtggt tctacgaagg atgcggtcat tgtgaatact gtaatagtgg taacgaaacg
    17881 ctctgccgtt cagttaaaaa tgccggatac agcgttgatg gcgggatggc ggaagagtgc
    17941 atcgtggtcg ccgattacgc ggtaaaagtg ccagatggtc tggactcggc ggcggccagc
    18001 agcattacct gtgcgggggt caccacctac aaagccgtta aactgtcaaa aattcgtcca
    18061 gggcagtgga ttgctatcta cggtcttggc ggtctgggta acctcgctct gcaatacgcg
    18121 aagaatgtct ttaacgcgaa agtgatcgcc attgatgtca atgatgagca gttaaaactg
    18181 gcaaccgaaa tgggtgcaga tttagcgatt aactcacgca ccgaagacgc cgccaaaatt
    18241 gtgcaggaga aaaccggtgg cgctcacgct gcggtggtaa cagcagttgc taaagctgcg
    18301 tttaactcgg cagttgatgc tgtccgtgca ggcggtcgtg ttgtggctgt cggtctgccg
    18361 ccggagtcta tgagcctgga tatcccacgg ctggtgctgg atggcattga agtggtcggt
    18421 tcgctggtct gcacgcgcca ggatttaact gaagccttcc agtttgccgc cgaaggtaaa
    18481 gtggtgccga aagtcgccct gcgtccgtta gcggacatca acaccatctt tactgagatg
    18541 gaagaaggca aaatccgtgg ccgcatggtg attgatttcc gtcactaaga ggcctttgct
    18601 gcgactgcca tgttcgggtc gcagcatcgc acactctcca acatgaaatg gctaaatgga
    18661 ttattgttgc gtaggaagga acaataatga tagtgttccc agtgaaggaa cgctactgag
    18721 agtaaagaca ctaatgaaaa tggccaatca tccccgcccg ggggacatta ttcaggaatc
    18781 actggacgaa cttaatgtca gcctgcgcga gtttgccaga gcaatggaaa ttgctccctc
    18841 aacggcaagc cgattgctga caggaaaagc agctttgacg ccagaaatgg cgattaaact
    18901 ttccgtggtg atcggcagtt cgccgcaaat gtggctgaat ctgcaaaacg cctggagtct
    18961 ggcagaggca gaaaaaacag tggatgtgtc gcgattacgc cgtctggtta cgcaataaaa
    19021 aaatggcact ttataaagcg ccatttagaa ctgatgacaa acgcaacatt gcctgatgcg
    19081 ctacgcttat caggcctaca cgttcactgc aatctattgg atttgaaggg gttgtaggcc
    19141 ggataaggcg ttcacgccgc atccggcatg aacaaagcct acgttgtcta caatatgaaa
    19201 atgacgcctt ttacagcgcc atttgaaact gatgacaaac gcaacattgc ctgatgcgct
    19261 acgcttatca ggcctacacg ttcactgcaa tctattggat ttgaagggtt tgtaggccgg
    19321 ataaggcgtt cacgccgcat ccgggatgaa caaagcctac gttgtctaca atatgaaaat
    19381 ggcgcctttt acagcgccat tttcagctta aagttcaaaa gagattatat cccttcttca
    19441 ctctcttttt tcgcttctgc cttctcgatt tcacgatacc agcgtggatg gtgtttcttc
    19501 gcccagcgac ggctcacctt cccttcgatc atccctttaa tcgacccttt cacccaaaat
    19561 gccatataca tatggatcag gatggcgtgg atcaggatga tacccgcagc cgcgtggatc
    19621 agcaggctgt agcgaacaac ctgcatcggg aagtactgcg caaagtacgg acgccagata
    19681 atcaccccgg tcaccagcag cacgaaaatc atgctcatga tcgaccagaa catcatcttt
    19741 tgcccggcgt tgtacttacc gacatccgcc actttatgct cattgccttt caatacttcg
    19801 acaatgttca acagccacgg aatatctttc ttatccggga tgttgtgatg cacaaaacgc
    19861 acaaacataa acatcagtgc gacgaaaatc gcaatgccga agaacgggtg caaaatgcgt
    19921 cccatctgcg gcgtaccgaa ggtttgcgtc agccattgca gcgtcgggaa gaagaacgaa
    19981 atcccggaca gcgccaccag gaagaagcaa atcaccacgg tccagtgaca ggcgcgatca
    20041 ataaatttgg tgcgcacaat cattttcgac ttactcatga tgatcctcct cgtcatcgtc
    20101 cacttcctta ttcgggccaa taccgatgta gtggaaaatc aacccggcaa aagtggcaat
    20161 aaagccagcc gctgccagcg gtttcaacgc gcctttccac aggcttaccg aggtgtcgat
    20221 cttcggatct ttcggcagac cgtgatacag ctccggctga tcggcgtgat gcagcacgta
    20281 cataacgtgc gtaccaccga ccccttccgg gttgtagacg ccagcatgtt cgtaaccacg
    20341 cgctttcagt ttcgccacgc gctgttccgc cagctccagc atctccttct tggtgccgaa
    20401 gtggatagcc ccggtcggac aggttttcac acaagccggt tcctggccga cgctgacgcg
    20461 atcgacgcag agcgtgcatt tatatacccg gttatcctct ttgttgaggc gcggaatatt
    20521 aaacggacac ccggcaatgc agtaaccaca gccgatgcag ttttccgact ggaaatcgac
    20581 aatcccgtta gcgtactgaa tgattgcacc agcagacggg cacgccttca ggcagccggg
    20641 atcttcacag tgcatacagc cgtctttacg gatcagccac tccagcttgc cgttctgttc
    20701 ggtttcgcta aagcgcatca ccgtccagga cttggcgctc agatcggcgg ggttatcgta
    20761 aaccccgacg cagtgcccca cttcatcacg gatgtcgttc cactccgaac acgccacctg
    20821 acaggcttta cagccgatac aggtggaaac gtcgataagt tttgcgactt ctgctttgta
    20881 atcacgcacc tgagaaggcg gcgtgatgga gttagttgcg gaccttttga taatgtcctg
    20941 cgtttccata gccatctgtt cgccccctta cgccttctcg atgttgacta agaacgcttt
    21001 atattccggc gtttgcgagt ttgcatcacc gacattcggc gtcagagtgt tagcgatata
    21061 acctttacgc gcgacaccct caaagcccca gtggattgga atacccaccg tttcaacctg
    21121 ctgaccattt acattcagcg gtttcagacg acgcgttacc acagccaccg cgcggataaa
    21181 gccacgcttg ctggagacag tgacacgatc gccattatta atgcctttcg ccgccgccag
    21241 cgtttcgctg atttccacaa actgttccgg ctgagcaatt gcgttgagca atgcgtgctt
    21301 ggtccaggtg tggaagtgct cggtcagacg ataggtcgta cccacatacg ggaactgctc
    21361 ttttttaccc atccgcagcg cgtcttgttc atacagacga acaaccgggt tagacaccac
    21421 gttcggatgc agcgggttag tgcccagcgg cgtttcaatc ggctcgtagt gttccgggaa
    21481 cggaccttcc gccattttgt tgatggcaaa caggcgtccc atcccttccg gctgcatgat
    21541 aaacggcccg gttggcgtac ccggtgcggc attgccgaag tcaggaatat cgttacccgt
    21601 ccacttgctg ccgttccact ggatcagcat ccgtttcgga tcccacggtt taccgttgat
    21661 atccgccgaa gcacggttgt acagcacgcg acggttgagc ggccacgccc aggcccatcc
    21721 cagcgtattc cccagaccgg acgggtcgga gttatcgcgg ttagccatct ggttgccctg
    21781 ctctgtccag ctaccggtgt agatccagca agaagatgcg gttgtaccgt catcacgcag
    21841 atgcgcaaag ctactcagca actgaccttt cttcgcaata agcacgccat tagcgttata
    21901 gagatcttcc agcgcgtagc cgttgttctc tttagccact tcgtcagatt gcggttcgtg
    21961 cggctgcttg tagttccagc tcatcttcat cagcggttct acgcctttac caccttcgga
    22021 ctggtacagc tcgcgcagat gatggtagat acccgccaga atttcaccgt cgttacgcgc
    22081 ttcgcccggc gcgtcctgac ctttccagtg ccactgcaac cagcgaccgg agttagcgat
    22141 agaaccatct tcttcagcaa agcaggtcga aggcagacgg aatacttcag tctgaataga
    22201 cgccggatcg acatcgttcg actcaccgtg gttctgccag aaggtagagg tttcagtcac
    22261 cagcggatcg ataaccacca tgtacttcag cttgctcagg cagctcacca ctttgttttt
    22321 gtccgggaag gacgcaaccg ggttaaagcc ctggcagaaa taaccggtga ctttgccttc
    22381 atccatcatg ttgaaatact tgatgacgtc gtaggtctgg tcccacttcg gcagccagtc
    22441 atagccccag ttgttctctt tctgcgcggc atcgccatag aaagatttca tcaggctaac
    22501 gaagaacttc ggatagttgc tccagtagtt cacctgatca gccagcgtcg ctttcggcgt
    22561 gttcgcttcc agatacgact gcaaatcaac ctgtttttct gacggcagcg tcagataacc
    22621 tggcaggctg gtagagagca ggcctaagtc agtcaagccc tgaatgttgg agtgaccacg
    22681 caatgcgttc acgccgccac cggccatacc catgttaccg agcagcaact ggatcatcgc
    22741 catagtacgg atgttctgcg cacccacagt gtgctgcgtc cagcccagcg cgtacaggaa
    22801 ggtggttgtg cgatccggtg cgctggtgga ggccagcact tcacacactt tcaggaagtc
    22861 ggcttttggc gtaccgcaga tgttttcaac gacgtccggc gtgtagcggg aaacgtgctc
    22921 tttcagcagg ttccacacac agcgcggatg agtcagtgtt tcatcgcgtt tcgcatagcc
    22981 gttttcatcg aactgatagt tccaggacga tttatcgtat tgacgttttt cagcgtcgta
    23041 gccgctgaac agaccgtctt cgaaagcaaa atcatcacgc accagcaggc tggcgttggt
    23101 gtaatgctta acgtattcgg cgttgatttt gttgttttcg atcaggtagc gcaaaacgcc
    23161 agacaggaac gtaatgtccg taccggaacg aataggcgcg taaatatccg ccacagaagc
    23221 ggtacgcgta aaacggggat cgacaacgat caaggttgca tcgttgttgt ttttcgcttc
    23281 catcgcccag cggaaaccga cgggatgcgc ttcagcagcg ttaccgccca tcaccatcac
    23341 gacgttagcg tttttgatat ccacccagtg gttggtcatc gcaccgcgac caaatgttgg
    23401 agcaagactt gctaccgttg gtccgtgtca gacgcgcgcc tggttgtcta ccgccagcat
    23461 cccgagggag cgggcaaatt tctgggtcag catcccggtt tcgttgctgg caccggaggc
    23521 acacagcata ccggtagaaa gccaacggtt taccgttacg ccctgctcgt tcttttcaat
    23581 aaagttagcg tcacggtcag ctttcatcag cttcgcaata cgggagaatg cttcttccca
    23641 gctaatgcgc tgccatttgt cagaacctgg cgcacgatat tccgggtagc gcagacggtt
    23701 ttcactgttg acgtaatcca gcaaaccggc ccctttcggg cacagcgcac cacggcttac
    23761 cggatgatcc gggtcacctt caatgtgata aatcgcttct ctggcgtttt ttgcgccatc
    23821 acccaggcta tacatcaata gcccgcaacc tacggaacag tatgtgcagg tgttacggat
    23881 ctctttagcg cgtaataatt tgtagtttcg cgcctgagcc agtgcttgct tcggggcaaa
    23941 gcccaatgcc gctactgttg ttccagccat accgcccgcg cagattttaa aaaattgtct
    24001 gcgactgacg tccattgctt tcctcttttt tcaggggtat tactgcgcga ggaaactaac
    24061 agtaaaattc tgaatctttt tgcgcgaaat caaaaagaac acgtgtttgc cacaaaataa
    24121 tccgcctgcg caaattgacc tacctcaata gcggtagaaa aacgcaccac tgcctgacag
    24181 gccagttaaa aaaatgctat aaaattcagc ttaattttta acggcaagag agacaaaaca
    24241 gcgagcatga cacgacaaaa agcaacgctc atagggctaa tagcgatcgt cctgtggagc
    24301 acgatggtag gattgattcg cggtgtcagt gaggggctcg gcccggtcgg cggcgcaact
    24361 gctatctatt cattaagcgg gctgctgtta atcttcacgg ttggatttcc gcgtattcgg
    24421 caaatcccga aaggctattt actcgccggg agtctgttat tcgtcagcta tgaaatctgt
    24481 ctggcgcttt ccttagggta tgcggcgacc cgtcatcagg cgattgaagt gggtatggtg
    24541 aactatctgt ggcccagcct gacaattctc tttgccattc tgtttaatgg tcagaaaacc
    24601 aactggttga ttgtacctgg attattatta gccctcgtcg gcgtctgctg ggtgttaggc
    24661 ggtgacaatg ggttacatta tgatgaaatc atcaataata tcaccaccag cccattgagt
    24721 tatttcctgg cgttcattgg tgcgtttatc tgggcagcct attgcacagt aacgaataaa
    24781 tacgcacgcg gatttaacgg aattaccgtt tttgtcctgc taacgggagc atgtctgtgg
    24841 atttactatt ttctcacgcc acaaccagaa atggtattta gcacgcccgt catgattaaa
    24901 ctcatctctg cggcatttac cttaggattt gcttatgctg catggaatgt cggtatattg
    24961 catggcaatg tcaccataat ggcggtaggc tcgtatttta cgcctgtact ttcctcagcg
    25021 cttgcagccg tgctgctcag cgccccgctg tcgttctcgt tctggcaagg cgcgctaatg
    25081 gtctgcggcg gttccctgct ctgctggctg gcgacacgtc gtggttaaaa taattgccgg
    25141 gtctacggcc cggcagttaa tcagataaat tccgacaata ttaaaaaata aatttcaaat
    25201 accagtaatt cacctta
//
LOCUS       DBANUU010000066        25059 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000066 DBANUU010000000
VERSION     DBANUU010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.066"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..158)
                     /locus_tag="ACIE68_002606"
     CDS             complement(<1..158)
                     /locus_tag="ACIE68_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFX5908905.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFG"
     gene            371..1162
                     /locus_tag="ACIE68_002607"
     CDS             371..1162
                     /locus_tag="ACIE68_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFX5908906.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDLPDADSVE
                     KLHITQQECIAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            1172..2014
                     /locus_tag="ACIE68_002608"
     CDS             1172..2014
                     /locus_tag="ACIE68_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFX5908907.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIAHQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            2024..2800
                     /locus_tag="ACIE68_002609"
     CDS             2024..2800
                     /locus_tag="ACIE68_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HFX5908908.1"
                     /translation="MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEIEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            2810..4351
                     /locus_tag="ACIE68_002610"
     CDS             2810..4351
                     /locus_tag="ACIE68_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFX5908909.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            4348..6429
                     /locus_tag="ACIE68_002611"
     CDS             4348..6429
                     /locus_tag="ACIE68_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFX5908910.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            6450..6926
                     /locus_tag="ACIE68_002612"
     CDS             6450..6926
                     /locus_tag="ACIE68_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFX5908911.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPL"
     gene            6969..8270
                     /locus_tag="ACIE68_002613"
     CDS             6969..8270
                     /locus_tag="ACIE68_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFX5908912.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIILIMGGVLSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFL
                     ESVITTAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIG
                     TFMDMTPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVS
                     KLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
     gene            8323..9294
                     /locus_tag="ACIE68_002614"
     CDS             8323..9294
                     /locus_tag="ACIE68_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFX5908913.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMKAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(9406..9855)
                     /gene="rplI"
                     /locus_tag="ACIE68_002615"
     CDS             complement(9406..9855)
                     /gene="rplI"
                     /locus_tag="ACIE68_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFX5908914.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAVANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(9897..10124)
                     /gene="rpsR"
                     /locus_tag="ACIE68_002616"
     CDS             complement(9897..10124)
                     /gene="rpsR"
                     /locus_tag="ACIE68_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFX5908915.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(10129..10443)
                     /gene="priB"
                     /locus_tag="ACIE68_002617"
     CDS             complement(10129..10443)
                     /gene="priB"
                     /locus_tag="ACIE68_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFX5908916.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQVITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(10450..10845)
                     /gene="rpsF"
                     /locus_tag="ACIE68_002618"
     CDS             complement(10450..10845)
                     /gene="rpsF"
                     /locus_tag="ACIE68_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFX5908917.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            11172..11447
                     /gene="yjfY"
                     /locus_tag="ACIE68_002619"
     CDS             11172..11447
                     /gene="yjfY"
                     /locus_tag="ACIE68_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFX5908918.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(11576..12262)
                     /gene="ulaF"
                     /locus_tag="ACIE68_002620"
     CDS             complement(11576..12262)
                     /gene="ulaF"
                     /locus_tag="ACIE68_002620"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFX5908919.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGKYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12262..12864)
                     /gene="ulaE"
                     /locus_tag="ACIE68_002621"
                     /pseudo
     CDS             complement(12262..12864)
                     /gene="ulaE"
                     /locus_tag="ACIE68_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
     gene            13052..14233
                     /locus_tag="ACIE68_002622"
     CDS             13052..14233
                     /locus_tag="ACIE68_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFX5908920.1"
                     /translation="MPLTVRQIESAKPADKDYKLSDGQGLYLLIKKSGARYWNLKYRF
                     AGKEKKLVIGVYPGISLALARQKRDEARKLLAEGKDPSYEKQASKHALRESIACTFCT
                     LAEELFTHMACRWTAGYLADQRARFENHINPMLGKRPVADIRPLEILNALRVIEKNGK
                     LEALRKTRQLCSQIFDYAIATGRATINPASTLNSVVLHPEKQQRRMLSEEQLGAFMYQ
                     MKVSGNFMSLVTQILVLTAVRTGELRLATWDEIDFTNALWVIPKERMKMRRPHVVPLS
                     HQTQMLLKEIKIITAPRNSELVIPGVYRDNQPRGRGCINEFLQQVGWHTFTTAHGFRH
                     TFSTILHDHDFSSAWIELQLAHVDKNIMRGTYNHAQYLDGRREMMQWYADYIDSLSEQ
                     A"
     gene            14516..15145
                     /locus_tag="ACIE68_002623"
     CDS             14516..15145
                     /locus_tag="ACIE68_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFX5908921.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     CDDVNVAVIDIAMPDENGLSLVKRLRAQKPQFRAIILSIYDTPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGMGGHYLCADAIRALRGGGQPAQALEILTPREREVFDLLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALNHHLLAGH"
     gene            15145..16686
                     /locus_tag="ACIE68_002624"
     CDS             15145..16686
                     /locus_tag="ACIE68_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFX5908922.1"
                     /translation="MSRNLRHWVISLFIVLAWGSGWLMLWTLSFYLTNNGQQAVLFLP
                     HGVYLAFLILHSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLLPAIIAQNV
                     WHRFPLYWQRLTLLLASVTAASLLNTAMLTPFMSGSIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLRLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKSLARELHDEIGQNITAI
                     QIQSQLVTRARDPAQVHSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFTYQLTTTPENETVRFTLYRLLQELLNNVCKHAEASEVTIILRQ
                     QGEILHLEVSDNGIGIASGKMTGFGIQGMRERVSALGGDLTLEKHNGTRVIVNLPTFL
                     QQIAG"
     gene            16759..18075
                     /gene="uhpC"
                     /locus_tag="ACIE68_002625"
     CDS             16759..18075
                     /gene="uhpC"
                     /locus_tag="ACIE68_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFX5908923.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGIGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKSTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWSGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            18072..19103
                     /locus_tag="ACIE68_002626"
     CDS             18072..19103
                     /locus_tag="ACIE68_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFX5908924.1"
                     /translation="MKMPLTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCETET
                     KEFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTERLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDNYGSTDVRKALINK
                     WVSEVKMGK"
     gene            19172..21250
                     /locus_tag="ACIE68_002627"
     CDS             19172..21250
                     /locus_tag="ACIE68_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFX5908925.1"
                     /translation="MSQTLALHPAKKRDAIFLWVLLGWLAFILLPSWSLDYGLLESTS
                     DEILEAYSWSHFNISWLWYLLPSLLLVRPFNEAKREQRSRHYFDAGWALLCIAFMVIS
                     ATIVGRGLGYAAIVLFIELSAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFANDAGEFAPLAFMNVLSQAHIIQVIVNSIVLSIAVGIGCTFFGLVLAIYT
                     TRIAKRSAVIGRIFSILPIVTPPFVVGLGVTLMMGRSGYITEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFSGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCVQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVCSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            21262..22308
                     /gene="fbpC"
                     /locus_tag="ACIE68_002628"
     CDS             21262..22308
                     /gene="fbpC"
                     /locus_tag="ACIE68_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFX5908926.1"
                     /translation="MSQKNFVELRNVTKRFGSNMVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRNEVKQRVKDALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQELYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAYFPVQGRGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDTGEHYYL
                     EIHPYGMFVLADSA"
     gene            22566..22804
                     /locus_tag="ACIE68_002629"
                     /pseudo
     CDS             22566..22804
                     /locus_tag="ACIE68_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(22994..23299)
                     /locus_tag="ACIE68_002630"
     CDS             complement(22994..23299)
                     /locus_tag="ACIE68_002630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908927.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWKAIYRSTSHYGKNVFIVATCVIIQVSLERPV"
     gene            23619..23813
                     /locus_tag="ACIE68_002631"
     CDS             23619..23813
                     /locus_tag="ACIE68_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908928.1"
                     /translation="MNENRIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRPDKKHFLECNEDK"
     gene            24174..24302
                     /locus_tag="ACIE68_002632"
     CDS             24174..24302
                     /locus_tag="ACIE68_002632"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908929.1"
                     /translation="MGADITEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(24613..24958)
                     /locus_tag="ACIE68_002633"
                     /pseudo
     CDS             complement(24613..24958)
                     /locus_tag="ACIE68_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         6113 a         6211 c         6246 g         6489 t
ORIGIN      
        1 ttaccaaagt gatgataaac cgtctgttta ctacaccccg cagccttaac aatattatcg
       61 accctggccc cttcataacc atattcggca aattcattaa ccgcgcataa cagcagcttt
      121 tccttcaggc tctgtacgtt gctggtatca agatacataa cgcttcccca cgacctcttc
      181 aagataacta tcttaacccg attaattaga agctccagta aaaaggataa aaacttgtac
      241 tttcatcaag ttatcaaatg tactgtgatc actatcgcat atcaaccaac cagatggttg
      301 atctttcttg cggcacgtcg caagctaatt gtcacttaga aaacaatatt caaaaaaaga
      361 gagctattgt atgaaatcca ctcgtcctgt tgccgtgatt accggagccg ctcgtggtat
      421 tggaaagggc tgtgcgctgg agcttgctcg tggcggattt aacctgctga ttaacgatct
      481 tcccgatgcc gacagcgtag aaaaactgca tatcacacaa caagaatgta tagctgaagg
      541 cgtggaagtg atttgcttcc ctgccgatgt cggcgatctc tccttgcatg aagagatgct
      601 cgacgcggcg caaaaccagt ggggacgtct ggactgcctg ctcaacaacg caggcatttc
      661 agtgaaaaaa cgcggtgacc ttctcgacct cgaaccagac agcttcgacc aaaatattgc
      721 cattaacacc cgcgcgccgt tcttcctcgc tcaggcattc agtaaacgtc tgctcgcaca
      781 gccgaaaccg gaagcagaac tgccgcaccg ctcgattatt ttcgtcagtt ccatcaacgc
      841 catcatgctg gcgatgaatc gcggcgaata taccattgcc aaaaccgccg tttctgccgc
      901 tgctcgtctg tttgctgctc gcctttgtaa tgaacaaatt ggcgtttatg aagtgcgccc
      961 cggtctcatc aaaaccgata tgactattcc ggcaacggct tattacgacg aactgattgc
     1021 caaaggtctg gtgccgtggg gacgttgggg ttatccggct gatattgctt ccactgtccg
     1081 cgcgatggcg gaaggtaaac tgatttacac ctgtggtcag gcggtcgcca tcgacggcgg
     1141 cttaagtatg ccgcgcttct gaggctgaat catgagagat ctgttacaac gcccggattt
     1201 attcagtatc aataccgcaa cactgggcta taaaacgccg ctacccgcca ttattgatgc
     1261 ctgcgccgcg cgtggtattg gcgcgattgc tccctggcgc agggagttgc aaggtgaaga
     1321 tctccagcaa atcgcccacc agcttgccgc cagtaacatg agcgtttccg gtttgtgccg
     1381 tagcacttac tacaccgcgc cgacactggc ggaacgcaaa ctggcgattg atgataaccg
     1441 ccgggcgctg gacgacgcag cggtgcttaa cgccgcctgt tatatgcagg tggttggcgg
     1501 tctgccaacg ggcacaaaag atctgtacga agcgcgggaa caagtaaaac aagggattcg
     1561 ccagttgctg ccacattcga aagatgtcgg cgtgccaatt gcgctggagc cactgcaccc
     1621 gatgacagct gccgatcgct cttgcctgtg cacgttacgc caggcgctgg actggtgtga
     1681 tgaactcgat cccgatggtg agtttggcct cggcgtggcg gtggatgttt atcacgtctg
     1741 gtgggatccg gatctcgcca gtcagatcct gcgtgccgga aaacgcatcc tcgcgtttca
     1801 tgtttccgac tggttagtgc cgaccactga tctggtcaat gaccgtggta tgccgggaga
     1861 tggcgtcatc aatattccgt caattcgtcg gctggttgaa aacgccggat ttaatggtgc
     1921 tattgaactg gaaatattct cgccttactg gtggcagaaa gatattaaca gcacgctgga
     1981 tattagcgtc gatcgcatcg cgcattattg ttaaggaatg atcatgagtg accaacccgt
     2041 tctgttcagc agagtggcgg cttcctgccg cctgacattg aaccgtgaag ataaatgtca
     2101 tgctattaac gaagagatga tcgaatctct cgaccattat ttaaatgaaa ttgaaaacga
     2161 caccacatta cgattagtcg aattaacggc aaccggcgat aaattctttt gtgcgggcgg
     2221 cgatattaaa tcgtggtcgg cctattcgcc attagatatg ggcagaaaat ggattaaacg
     2281 cggtaatgac gtttttaatc gcctgcgaaa cttaccccaa ttaaccgttg cgaatctcaa
     2341 cggtcatacc atcggcggtg gtattgaact ggccttgtgc tgcgatattc gtattgcccg
     2401 ccccggcgcg aaattctcta acccggaagt gatgctcggc atggtgcctg gctggatggg
     2461 cattgaacgt gtgcttaatc aggttggtcc ggtggtcggt cgccagatgc tgatgctggg
     2521 taaacgcctg acggcgcagg aagcccaggc ggcgaattta attgatgaag tggtagaaat
     2581 agaacaggtg gaaagctgga tggcgaacca gttagcgcag ctggaaaaat gcggtccggt
     2641 ggcactggcg catattaagc aactgattct ggcactggaa aataaacatg ccgattaccc
     2701 gcaccagcta ctggccggat taatgtccgc cactcaggat tgccagcagg cgacgcgcgc
     2761 ctttgccgaa aaaagtagcg tgagcttcca taatcaataa ggaaattgta tgcgtaagat
     2821 aacaaccgcc gaggcactgg cggcgcaaat tcaggacggc gcaaccattg ccattagcgg
     2881 taacggcggc ggcatggtgg aagccgacca tattctggct gctattgaag cgcgtttcct
     2941 gcaaaccgga caccctcgcg acctgacgct aatccactcg ctgggtattg gcgatcgcga
     3001 ctgcaaaggc actaaccgtt ttgcgcacgc agagatgctc aaacgcatta ttgccgggca
     3061 ctttacctgg tcgcccaaaa tgcaggcgct ggtaaaaaat aacacgattg aagcctactg
     3121 ctttcctggt ggcgtgattc aggcactgct acgggagatc ggcgccggac gtccggggct
     3181 ttttacccac gttgggctgg gatcgtttgt tgatccacgc aatggcggcg gtaagtcgaa
     3241 tgaatgcact accgacgacc tggtagaact gattgaaatc gacggtgaaa ccaaacttcg
     3301 ttatcgccct ttcaaagtgg attacgcgat tttgcgtggc acttatgccg atcctcgagg
     3361 caacgtcagc cttgaagaag aagcgattga tatggatagc tattccatgg cgctggcagc
     3421 acacaacagc ggcggcaaag tgttcgtaca ggtacgcgat gtgctggaag ctggcgcgat
     3481 tgaaccacgc cgggtcaaat taccgggcat tctggttgat ggcatcgttg aacaccgcga
     3541 acaaccacaa acctatcttg gcggttacga cctgaccatc agcggccaac atcgccgtct
     3601 gagttctaac gacgccattg aactggttag tcatccggta cgtcgcctga ttgcccgtcg
     3661 ggcggcacgc gaactggtag ctggcgcttc aaccaacttt ggctttggta ttcctggggg
     3721 tattccaggc gtagcgctgc gcgaaggcgt gccttaccaa agtttatggc tgagcgtcga
     3781 gcaaggcgtg cataacggta tgatgctgga tgatgcgttt ttcggctgcg cccgcaacgc
     3841 cgatgccatt attccatcac tcgatcaatt tgagttctac agtggcggag ggatcgatat
     3901 caccttcctc ggcatgggag agatggatca gtacggtaac gtcaacgtat cccatctcaa
     3961 tggcaatctc attggtcccg gcggttttct cgaaattgcg caaaacgccc gtaaagtggt
     4021 gttctgcggc acgttcgacg ccaaaggtag caagattgat gtaacgccag atggcttgca
     4081 tatcacccag tcaggtcaaa tccctaaact ggttacccag gtggaaaaaa tcacttttag
     4141 cgccgcctac gcacagcaaa gtggtcagga agtgttgtat atcactgaac gcgcagtatt
     4201 ccagttaacg gcagaaggcg ttgaattaat tgaaatcgcg ccgggtgtgg agattgagcg
     4261 cgacattctg ccgtatatgg ctttccgtcc aattatcaag cacccacgcc tgatggaaag
     4321 tagcctgttt acgccgatgg aggatgcatg agtcagcttg atgacaccat ccttgatgcg
     4381 ctgacgcacg ttactttccc aaagggtttt gcacaggcag agcccgcatg ggttgtcacg
     4441 gtagacggtg ttgattaccc actctggcaa acagatgccc tggtagtcgg cagtggtgca
     4501 gcaggattac gtgcggcagt tgaacttaaa cgccgccagc aaaatgtgct gatcgctacc
     4561 gccgggttat atatggggac gtcggcatgt tctggttccg ataaacagac actatttacc
     4621 gccgctaccg cgggcaacgg cgacaacttc accaaactgg cagaggcact ggcgagcggt
     4681 ggggcgatgg atcacgacac cgcttatgtc gaagcggtag gttctctgca cactctaggc
     4741 gggttgcaat atcttggtct ggaattaccg gaagatcgct atggcgcgat tcttcgttat
     4801 caaaccgacc atgacgaagc cgggcgtgca acctcgtgtg ggccgcgtac ctcaaggtta
     4861 atggtgaaag tgctgttgga agaagtacag cgcctcgcca ttccagtgtt gaccagtgca
     4921 acagtgatta aactgctgca tcagcgtgac gaaaacggcg aagaccgcgt ggcaggggca
     4981 atcctcgcga ccggtcatcg cgcccataac ccatgggggc tggcaattgt gactgcgccc
     5041 aatgtggtac tggcaacagg agggcctggc gagctttatc gcgacagtgt ttatccacat
     5101 aaatgcttcg gctcgctggg gctggcgctg gaggaaggtc tgacgctaac caatctgacc
     5161 gaaagccagt ttggtatagg cacgccgcgt agcacgtttc cgtggaattt atccggcacc
     5221 tatgtacagg tgatcccgta tatctattcc gtggatgctg agggtaacga gtataacttc
     5281 ctcgcggatt actatcgcac cacccaggag ctggcttcaa acattttccg taaaggctat
     5341 cagtggccgt tccacgccac gcgggtgatg gattttggct ccagcttgtt agatatggca
     5401 gtagcgcaag agcagcaatc agggcgtcag gtatttatgg atttcaatcg caatcctgaa
     5461 gcggtgccgg gtgacctgcc gttctcatta gatcgactgg acgacgacgt tcgcgcgtat
     5521 ctggaaaata acgacgctct ggcaccatcg cccatcgaac ggctgcaacg aatgaatccg
     5581 ctgtctatct cgctgtataa gatgcacggt tacgatctca ccacacagcc tttgcagttt
     5641 gccatgaata atcagcatat gaatggcggc attgaagtgg atatctgggg acaaacctcc
     5701 ctgcccggct gtttcgccgt gggggaagtt gctggcacac acggcgtcac tcgccctggt
     5761 ggtgcagcat tgaatgccgg gcaggttttt gctgttcgtc tggcacgttt tattggttgc
     5821 acgcaaaagc ggaatattga tggagatata gcacagctgg tagctcctac actggcttct
     5881 ataagagaga tcattactca ggcgcacgat aacgggaccg ggatgccgtt gtcggttgtg
     5941 agagaaaaaa ttcaggcacg aatgtctgac catgcgggat ttatttgcca tgccgataaa
     6001 gtccgacgcg ccactcgcga tgccctgcta ttgagcgaat ttgtccaacg gcatggattg
     6061 gctatcaaac atgtgggtga agttgccgag ctgtttatgt ggcggcatat ggcactgact
     6121 tctgccgccg tattaacgca attaacgcat tatattgacg caggtggtgg tagtcgggga
     6181 gcacgtatta ttctcgacag ggatggaaat tctattccgc aaacacgaaa tggtttttgt
     6241 gatgcctggc gctttcgttc agagcgtact gaggataaaa aagataaact acttattcat
     6301 tattgcaacg gtatctttca cgtcagagaa acacctgttc gtgagtttcc tattatccga
     6361 ggtatttggt ttgaaaaaaa ctggcctggt tttcttaatg ggacaattta ccagccacag
     6421 gatgaatgat attgcaaagg ttaatcacta tgcgaaatat aattagatct attaatgctc
     6481 tgcttgcagc attgaacatt accattttgg cgatcatcgt ggcatgtgtg acgtggcagg
     6541 tggctgcacg atttatattt actagcccca gtatttttac agatgaactc agccgtttgc
     6601 tctttatttg tcttggactt tttggtgggg catacaccgc agggcaaaac cggcatctgg
     6661 cgattgacct gctacctatg atgctgaaag gaaaagcccg acgtcattta tttttgtgta
     6721 ttcagattat cgttattatc ttcgcaacca tcattatggt atatggtggt gggctattaa
     6781 cgatggatac attcgattca ggtcagacct cgcccgcttt aggctggcaa atgggatata
     6841 tctatatgag catcccgatt agtggtgtac ttattattat atacaccata gatatggttt
     6901 taaccgaact taaacaacca ctctaaagat aacaaacaac aaccaaccat agtaggaatg
     6961 gagaaattat ggattactgg ttaccgatta ttgtgttatt tggtgccttc ttttttatgt
     7021 tggcattagg tgtacctatt gtttatgcca ttggtttatc gacactggca tcaatttcca
     7081 cacaactcga tttcaactca gcattgagtg tggtttccca aaagttagcc tccgggttag
     7141 atagttttac gttgttggca atcccatttt ttatcttgtc tggcaacatt atgaaccatg
     7201 gaggaatagc gcgacgatta atcaactttg cccgtattct cggcggtcgt ctgccaggct
     7261 cacttgccca ctgtaatatt ctcgccaata tgctgttcgg ggcaatttct ggttcagccg
     7321 tggcttcggc ggctgcgatg ggcggcgtca tgcatccgca acaggttaaa gagggttacg
     7381 acccggcatt cagtactgcg gttaacgttg cctctgcccc gacgggtttg cttattccac
     7441 caagtaatac gttaatcgtc tattcactgg tcagtggtgg tacttctatt gccgcactgt
     7501 tcctggcggg ttatgttccg ggaattttgc tgggtctggc actgatggta atagccggta
     7561 ttatcgcggt acgtcgcggt tatccaaagc cagaacgccc gactttacgc caggctggcg
     7621 ttgcgatatg gatggctatc cccagtattt tcctgattat tttaattatg gggggtgtat
     7681 taagtggtat ttttaccccg acagaggcct ccgcaattgc agtaatttat acccttttcc
     7741 ttgcactggt tctttatcgt gaaatctcag ttaaagattt acctaaaata tttctggaat
     7801 ccgttattac cactgcaatt gtcttgttat taattggttc ttcgatgggt atgtcatggg
     7861 caatgtcaaa cgctgacgtt cccttcctga ttctcgattt gcttaacact atttctgaca
     7921 acccaataat aatcctgctt attatcaata ttatcctgtt gattatcggc acttttatgg
     7981 atatgacacc ggcggtactg atttttacgc cgatattttt accggttgtc accgaactgg
     8041 gcatggaccc gattcacttt ggtattgtca tggtactgaa catgtgtatc ggtatttgta
     8101 ctccgcctgt aggcagtgtg ttatttgtcg gttgtagtgt atctaaatta ccgattaata
     8161 aaattatcaa acctatgctg ccattctatg ccgtgatggt attggtgctg gcaatggtga
     8221 cctatattcc gcaaatcagt atggcattgc caagagcact gggctactga tgcgcattca
     8281 ctggcggcac ctgccgccat atttattaga aaaggaacat ctatgaaaat aaaagtatcc
     8341 gctggcatta tcggtgctgt tcttatgtta tccgcaagcc agtcctgggc agtgacatta
     8401 aaactgagtc ataatcagga taagtctcat cctgttcata aagcgatgga gttctttgcg
     8461 aaaaagagca aagagtactc taacggtgat attactattc gtatttatcc aaatggaaca
     8521 ttgggtactc aacgagaaac aatggagctg attcgttctg gcgctattcc cctggtaaaa
     8581 accaatgcgg cagaaatgaa agcatttgaa aattcctata aattatttag cctgccttat
     8641 ttgttccgcg atcgtgatca ttattatcag gtcatgcagg gcgatatcgg gagaaaaatc
     8701 ctcgactcaa cgaaaagcaa aggttatttc gggctgactt tttatgatgg aggcgcccgc
     8761 agtttctatg gcaataaacc agtactgaaa ccagacgatc tgaaaggcat gaaagtccgt
     8821 gtccagccaa gccctggcgc agttgaaatg atcaaagtca tgggcggtaa cccgacgcca
     8881 ctggattacg gcgagttgta tacagcctta cagcagggtg tggtcgatat ggcagaaaac
     8941 agcgtgatgg cgctgactac catgcgtcac ggtgaagtgg caaaatcctt cagccttgac
     9001 gaacacacta tggttcccga tgtggttctg atgagcaatg ctgcgtttga taaacttagc
     9061 ccggaaaatc aggcagttat attaaaagca gctaaagaat caatgagcta catgaaagac
     9121 ttgtggagcg aggaagagaa acaagaattt gcaaaactgg ataaaatggg cgtgaaagtc
     9181 taccaggtag ataaagctcc gtttatcgag aaagtacagc caatgtacgc aaacttcgct
     9241 aaggacaacc cagcccttgc cccaatgctg gctgatattc aggcagctaa gtaattatct
     9301 tcgtagggct gcttattgtg gcccttatcg atatctgcaa gtgctcgcta caaaagcaaa
     9361 acgccgacca atggtcggcg tttttacgtc tcgttgaata acgaattatt cagctactac
     9421 gtttacgatc actttcgcga atacttcgct gtgaacctgg aagctcactt cgtgttcgcc
     9481 agtggtacgc agaacgccgt tcggcagacg aacttcgctc ttagccactt caacgccagc
     9541 tgcagttaca gcgtcagcga tgtcgcgagt accgatggaa ccgaacagtt taccttcgtc
     9601 gccagcttta gacgcgatgg taacagtttc cagtgcattg attttctcag cgcgagcatt
     9661 agcaactgcc agaacttcag ccagtttagc ttccagttca gcgcgacgtg cttcgaagaa
     9721 ttcaatgttt ttcttggtag ctggaacagc tttaccctgc ggtaccagga agttacgagc
     9781 atagcccgct ttaacgttta cctgatcacc caggctaccc aggtttgcta ctttatcaag
     9841 cagaataact tgcattacct tatcctctca aagtcgtatt aatggaccgt gaccgattac
     9901 tgatggcgat cagtgtacgg cagcagggac aggtagcgag cgcgtttgat agcgcgagcc
     9961 agctgacgct ggtattttgc acgggtaccg gtgatacggc ttgggacaat cttaccgctt
    10021 tcggtgatgt agtttttcag cgtagcgata tctttatagt cgatctcttg aacgccttcc
    10081 gcggtgaaac ggcagaactt gcgacgacgg aaataacgtg ccatatggct agtctccaga
    10141 atctatcaat tcaatctgct cggcatgcaa aaccattttg ctcagtccgt tctttgcctt
    10201 gtggcatgaa atgaacccct gaacggttat gcgactgccg accgttatac tgtgagtaat
    10261 gacctggttt tcgtgtccgc taacaataac gggcatttga caccatgcct gccggtgaaa
    10321 gccggcttcc tcctgcacag aacgatgctc aagcacgaac tggcagtgag gaattcctga
    10381 tggactgacc tttcgaaggg gagtcctgca cacggtgccg gacaacacca gacggttggt
    10441 catcagaaat tactcttcag aatccccagc ttcagcatca tcagcggttt cgtttgcgaa
    10501 atcatcgcga cgctcacggc gctcgtcttt cgctttaacc atcggagatg cttcggtaac
    10561 agcgtgcttg gtacgcataa ccatgctgcg gataacggca tcgttgaagc ggaaggtagt
    10621 ttccagctca tcgatcactt cctgcggagc ttcaacgttc atcagaacgt agtgtgcttt
    10681 gtgcagtttg ttgatcgggt aagccaactg acggcggccc cagtcttcca gacggtggat
    10741 cttgccttct gcaccagtga tggcagcagt gtagcgctcg atcatgcccg gaacctgttc
    10801 gctctggtca ggatggacca taaaaacgat ttcgtaatga cgcatcgaat tgctccttac
    10861 ggattattca gcctcctgtc tgggtcagcc gaatcccggg gaggcaagga acgtgttaaa
    10921 ggtcggctga aaaatgacgc gttatagtac ttgtatgccc cgctaaactc aaggccagcc
    10981 gacaaataaa tctcatcttt cgctcaaaag aactaactcc ctgatttgaa gttcatcaca
    11041 ttccgtcaca gtgttttttc gaacaactgc atcagaaatg gtcaccatca acttctttat
    11101 cattgattaa aattaaatca gaagatcatc acagaggaga gcacacaaag tcagccgtcg
    11161 aggaggtatc tatgttcagt cgtgttttag cccttctggc tgtgcttttg ctaagtgcaa
    11221 atacatgggc agccattgaa atcaataacc accaggccag aaatatggac gatgtgcaaa
    11281 gcttaggcgt gatttatatc aatcataatt tcgccactga aagtgaagca cgtcaggcat
    11341 taaatgaaga aacagatgcg cagggcgcaa cgtactacca cgtaattctg atgcgggaac
    11401 cggggagtaa cggcaatatg cacgccagcg cggatattta tcgctagcac caggtataac
    11461 caacgaaaca ttgccatagt ttgctttgcc cccttcgcag ggggcttttt ttgaaaaacg
    11521 gctgcggaat tagaccagtt atctcccgaa gaaggaaatt tccgcagcgc gtgttttact
    11581 tctgcccgta ataagcgtta ggaccatgct tacgcatgaa gtgtttattc atcaggaagc
    11641 tgtcgatgtg attgagttgt ggattaatac tgcgcgcaat ccacgccatt ttcgccactt
    11701 cttccatcac taccgcgtta tgcaccgcgt cgtgggcatc tttcccccag gcgaacggtc
    11761 cgtgctgata caccacaatt cccggcgtat gcagcggctc ggcgttgccc agcgtttcga
    11821 taatcacttt gccagtgttc agttcatact caccctgcac ctcttcttcg cttaatccac
    11881 gcgtacacgg gatgtcacca aagaagtagt cggcgtgcgt ggtgcctaac gccgggatcg
    11941 ccagccccgc ctgcgcccat gcggtggcat gagtggaatg ggtatggaca atgccgccga
    12001 gcgacgggta acgacggtag agttcaagat gcgtcgcggt gtcagaagac gggcgatact
    12061 tcccttctac caccttgccg ctcatatcga ccaccaccat atcgtctgct ttcatggttt
    12121 cgtaggcaac gccgctgggc ttgatcacca ccagcccgcg ttcgcggtcg atagcgctga
    12181 cgttgcccca ggtaaaggtc accagcccat agcgcggcag atccatgttg gcttcaaata
    12241 cctgctgttt tagcttttgc attatgccgc ctccaccatg cccgctttcg ccatgcgcgc
    12301 tttcacccaa tcacgcgctt tcgccacttc tgccgccggg tcttccgccg tttcgctcca
    12361 catctcaatc aggtacggcc cgcaatagcc actctgtttg agcgtttcga aacaacgttc
    12421 gaaatccact acaccttcgc caaacggcac gtttttgaag acgccaggtt tggtgtcttt
    12481 cacatgtacc gcgacgatat gcccgattcc ggcctgcaac tccatctgca catcgttgtc
    12541 ccacgccgac aggttgccga tatccgggta gagctggaac cacgggttgt tgagatagtg
    12601 cgcgtaaccc agcgccttgc tgatggagtt catcaacgga taatccatga tctccatcgc
    12661 cagcgtcacc tgcgcgcggc tcgccatctc aacgctctct ttcaggccgt cacggaaacg
    12721 acgacgcgtt tcgttattgg cttcctgata gtaaacgtca tagcccgcca gctggatcac
    12781 gcgaatgccg acgtcctggg cgaactggat agctttacgc ataatctcca gcccctgcgc
    12841 ccgcaccgcg tcatcttcac tgcccggcgg ggaggattta ttaagccctg tttatttcca
    12901 ctgatttcaa cagaactctc tccaccccct ttaactcact ttaaaataag gtattatcat
    12961 aaattctttt actctggacg taaaccatcc tccatgatca tcaatataat tttgggggct
    13021 actctggggg ctacttttcg gagttaacat catgccactt actgttcggc aaattgagtc
    13081 ggccaaacct gctgacaagg attacaaact ttctgatggt caaggacttt accttcttat
    13141 caagaaatct ggcgctcgtt actggaatct gaagtatcga tttgcgggta aagaaaagaa
    13201 attggtgatt ggtgtctatc caggtatatc actggctctg gcgcgtcaga aacgggatga
    13261 agcacgtaaa ctcctcgctg aaggaaaaga tccttcatat gaaaaacagg cgagtaaaca
    13321 tgcactaaga gaaagtattg catgcacatt ttgtactctt gcagaagagt tgttcacaca
    13381 tatggcttgt cgttggactg cgggttattt agcggaccag cgtgcaagat tcgagaatca
    13441 tattaatccc atgctaggga aacgtcctgt ggctgatatt cgccccttgg aaatattgaa
    13501 tgcactgagg gttatagaaa agaatggcaa acttgaagcg ttacgtaaga ctcgtcagct
    13561 ctgttctcaa atatttgatt acgccattgc gacggggcgc gccactatta acccagcatc
    13621 gactctcaac agtgttgttc ttcatccaga aaaacagcag agacggatgc tatctgaaga
    13681 acagttaggc gctttcatgt accaaatgaa agtttcaggc aattttatgt ctctggtaac
    13741 gcaaatactg gttctaactg ctgttagaac gggagaatta cgccttgcta cttgggatga
    13801 aattgatttt accaatgcct tatgggtaat cccaaaagag agaatgaaaa tgcgaaggcc
    13861 gcatgttgtt ccactttctc atcagactca aatgttattg aaagagataa aaataatcac
    13921 agctccccgt aactccgagc tggttattcc tggtgtatac agagataatc aacctcgtgg
    13981 tcgcggttgc atcaacgagt ttcttcaaca agttggctgg catacattca ctacagctca
    14041 tggttttcgg catactttca gtaccattct ccatgaccat gatttcagct ctgcctggat
    14101 tgaactccag cttgcccatg ttgataaaaa catcatgcgc ggcacctaca accacgcaca
    14161 gtatctcgat ggtcgtcgtg aaatgatgca atggtacgct gactacattg attcactttc
    14221 tgagcaggcc tgatgaatga aagttcagtg atgcagaaat aatgaataac catccggctt
    14281 tggaggccgg atggttttat ttatgttttc ttttttattt gctcacatct tctgaactgc
    14341 ccctgcagac aataatctga acaccagata tagagccact catgaaataa agcataatta
    14401 tattgaataa ccaattacat tttttgttac atccattcca tatctgtcgc tggttgattg
    14461 ccagcgacgc gtcgtgatgc atgataggag aatgagataa taagagaaga aaagtatgat
    14521 tcgggttgtg ctggtggatg accatgtggt ggtgcgctct ggtttcgcac agttactctc
    14581 tctggaagac gatctggagg tcattggtca gtacagtagt gccgcacagg catggtcggc
    14641 attaatctgt gatgacgtca atgttgccgt cattgatatt gctatgccgg acgaaaacgg
    14701 cctcagtttg gttaagcgcc tgcgtgcaca aaaaccgcaa ttccgcgcca ttattcttag
    14761 catttatgac acgccaacct ttgtacaaag cgctctggat gctggtgcca gtggctattt
    14821 gaccaaacgc tgtgggccgg aagagttagt gcaggcggtg cgctctgtcg gtatgggagg
    14881 gcattatctc tgcgccgatg caattcgggc tttacgcggc ggcgggcaac ccgcccaggc
    14941 actggaaatt ctcaccccac gagaacgtga agtgtttgat ttattagtca aaggcgacag
    15001 tgtaaaagag attgccttta agctcgaact cagccataaa acggtacatg tacatcgcgc
    15061 caacgtgctg ggaaaactga actgtcatag caccattgag ctggtgcatt ttgcactcaa
    15121 ccaccatctg ctggcggggc attaatgtcg cgtaaccttc gtcattgggt gatttcgctg
    15181 tttattgtgc tggcctgggg aagcggctgg ttgatgttgt ggacgctcag cttttatctg
    15241 accaataacg gtcaacaggc agtgttattt ctgccgcacg gagtttacct cgcttttctt
    15301 attttgcact ctcgccgtta ctggcctgcg ttggtgctcc cccctttgct gatgatgttc
    15361 tggctgcaca gcgagcaact gttgaacggc tatctcatgc tggcaacgcc ggttattagc
    15421 ctgcttcctg ccatcattgc acaaaacgtc tggcaccgtt ttccacttta ctggcagcgt
    15481 ctgacgttgc tgttagcctc agtaacagca gcttcattgc tgaatacagc catgctaacc
    15541 ccttttatgt caggctcaat aatgctgccc ggtctgacct catttaccgg cggcgtattg
    15601 ttgacgccgt ttgtctatct tatctttgag tttctgcgcc agcagcatcg ctatcagctt
    15661 ctgagactcg atacgcataa tccccccttg cgaacctctt taatcatctg gtgtagccta
    15721 ttttttatta ttggtatcgg cacccagatg gtgttgtcgc ctgaaatcga acgcctgctg
    15781 ctgatcgtgg tatttctacc caatgtagtc atggcctgga aatttggctg gcaagggggc
    15841 gtactctcgg ggcttttggg cagcatgatg atcactattg cccgccagat tggtgtgggc
    15901 tttagtaacc tggtggaact ggagattttt ctcgcaaccc aggcattgct gggaattggc
    15961 ctgggcattg ccattagtcg gcagcaacat ctggcgctaa acctgcatca ttaccgtcag
    16021 cgactggagg cagaactggc agcacggcgg gcattggctg aaaagctgat ccataccgag
    16081 gaagacacgc gtaaaagcct ggctcgcgag ctgcacgacg aaatcggtca gaacattacc
    16141 gccattcaga tccagtcgca actggtgacg cgggcacgcg atccggcgca agttcattcc
    16201 gcagccagcc aaatcaacga actcgcccgc cgcattcatc tctccacccg ccagttgctg
    16261 cgccagttgc ggccccccgc gcttgatgaa ctgacattcc gcgaagcatt acttcatctg
    16321 attaatgaat ttgcctttag tgaacgcggc attcattgcc agtttaccta tcaactaacc
    16381 accacgccag aaaacgaaac ggttcgcttt acactgtatc ggttattgca ggagttactc
    16441 aataacgtct gtaaacacgc tgaagccagc gaagtgacga ttattctgcg ccagcaggga
    16501 gagatattgc acctggaggt cagcgacaac ggtataggaa tcgccagcgg gaaaatgacc
    16561 ggatttggca ttcaggggat gcgtgaacgc gtcagtgccc tgggcggcga cttaacgctc
    16621 gaaaaacata acggtacgcg ggtaattgtt aacctgccca catttttgca acaaatcgcc
    16681 ggataaccag gaaaaagtct tagtcatccc ggattttctc tcatcctctt tcactttcat
    16741 tttcatatag tcagcaacat ggagacgacc atgcacgcgc gttcagcgag agagattaat
    16801 caatgctacc ggacattacg tccacgattg ctgatgtgca tgattattgg ctacgccgca
    16861 ttttatctga cgcgaaaaag cgtgaattac gtccttccgg cgctgcaaac cgacctcgga
    16921 ctggataaag gcgacattgg cctgctgggt tcactgtttt atctcagcta cggattgtcg
    16981 aaatttaccg ccggactgtg gcatgacagc cacggacaac gcggttttat gggtatcggc
    17041 ttgttcgcca ctgggctatt gaacgtggtg tttgcctttg gcgaatcact tacgcttctg
    17101 cttgtggtct ggacactgaa cggatttttt caaggctggg ggtggccacc gtgtgcccgc
    17161 ttactgaccc attggtattc ccgtaatgaa cgtggtttct ggtggggctg ctggaatatg
    17221 tcgatcaaca ttggcggagc gattattccg ctgattagcg cctttgccgc ccactggtgg
    17281 ggctggcagg ccgcgatgct gacaccgggg ataatcagca tggcattagg gatctggtta
    17341 acgctacaac tcaaaagcac gccccaggaa gagggattac cgacagttgg tcactggcgt
    17401 cacgatccgc tggagttacg tcaggaacaa caaagtccgc cgatggggct gtggcaaatg
    17461 ttgcgcacca ccatgctgca aaatccgctg atctggctac tgggtgtctc ttatgtactt
    17521 gtttatgtga tccggattgc gctaaacgac tggggcaata tctggctgac ggaaagccac
    17581 ggcgtcaacc tgctcagcgc caacgccacg gtaatgctgt ttgaagtagg cggcttactc
    17641 ggcgcgttat ttgccggatg gggttcggat ttgctgttta gcgggcagcg agcccccatg
    17701 attttgctgt ttacccttgg gttgatggtt tcggtcgctg cactctggtt ggcaccggtt
    17761 catcactatg cccttctggc tgtgtgcttt tttactgtgg gcttttttgt ctttggcccg
    17821 caaatgctga ttggacttgc ggcagtggaa tgtggacaca aagctgcggc gggttcaatc
    17881 accggatttc tcggcctgtt cgcctatctc ggtgcggcgc tggcaggctg gccgttgtcg
    17941 ctggttatcg aacgctacgg ctggtcggga atgttcagtt tactctcggt cgccgcagta
    18001 cttatgggtt tactgctgat gccgttactg atggcgggca tcaccaccac tcacgctcga
    18061 aggataaaac aatgaaaatg ccccttacct ctactctgat tgcttctgcc gttgcgctgg
    18121 caaccttaac gggtgccgcg caggcaaaag gccggctggt ggtctattgc agcgccacta
    18181 atgaaatgtg tgagaccgaa accaaagagt ttggcgagaa atatgatgtg aaaacctcgt
    18241 ttatccgcaa cggctccggt agcaccctgg caaaagtcga tgctgaaaag aaaaacccac
    18301 aggctgacgt ctggtacggc ggcactttag acccgcagtc gcaggccggc gagatgggcc
    18361 tgttacaggc atataaatcg cctaaccttg aacagatcat ggaaaaattc cgcgatccgg
    18421 caaaagtgaa aggaaacctt tcctccgccg tttatgtggg gatcctcggg tttggcgtca
    18481 acacagagcg tctgaaagag aaaaacctgc ctgtgccgaa gtgctggaaa gatctcacca
    18541 aacctgaata taaaggcgaa attcagattg ccgatccaca aagttccggt accgcctaca
    18601 ccgcgctggc tacctttgcg caactgtggg gggaagatca ggcctttgat tatctaaaac
    18661 aacttaacgc caacgtctcc cagtacacca aatcaggcat tgccccggca cgtaacgccg
    18721 cccgcggcga aacggcgatt ggtattggtt tcctgcatga ctactcgctg gaaaaagaac
    18781 agggcgcacc actggagttg atttccccgt gtgaaggtac gggctacgaa ataggcggcg
    18841 tcagcattct gaaaggtgcg cgcaacctcg acaacgccaa actgttcgtg gactgggtat
    18901 tatcaaaaga agctcaggaa ctggcgtgga agaaagggaa atcttatcag atcctgacca
    18961 ataccaccgc cgaaacgtcg ccaaattcgc tgaagctcga cgacctgaaa ttaatcaact
    19021 acgacatgga taactacggt tcaactgatg ttcgtaaggc attaattaat aaatgggtca
    19081 gcgaagtgaa gatgggtaaa taaacccgta ttgtgtgcac ttgccgggtg cagcacagac
    19141 gcctgccccg gccaaccata ccggaactgc tatgtcacag acacttgcac tccatcccgc
    19201 gaaaaagcgg gatgcgattt tcctttgggt tttgctgggc tggctggcat ttatcctgtt
    19261 gccgagctgg agcctcgatt atggtctgct ggagtccacg agtgatgaga tcctcgaggc
    19321 gtacagctgg tcgcatttta atatcagctg gctgtggtat ttactcccga gtttgctgct
    19381 ggttcgcccg tttaatgagg ccaaacgcga gcagcgtagc cgtcattatt ttgatgccgg
    19441 ctgggcattg ctttgtatag cgtttatggt catcagtgcc accatcgttg gccgcggtct
    19501 aggctacgcc gccatcgtgc tgtttatcga attaagcgcg ataatgacgc tggccttaac
    19561 ccgcctcgaa tggctgggtg gcgaccgctt tgtgatcggc tcgctgattg cgattgtggc
    19621 gctgattggc atttttatcg tctggccgag catcgccatt tttatcccga tgttcgccaa
    19681 cgacgcggga gagttcgccc cactggcgtt tatgaatgtg ctctcgcagg cccatatcat
    19741 tcaggtgatc gtcaactcta tcgtcttatc gatagcggtg ggtattggct gcactttctt
    19801 cggtctggtg ctggcgatat ataccacgcg catcgctaaa cgcagcgccg taatcggccg
    19861 catcttttcc attctgccaa ttgtcacgcc accgtttgtg gtcggcttag gcgttacgct
    19921 catgatgggg cgctccggct acataacgga atttatggtg gagtggtttg ggctgaccaa
    19981 caccaactgg ctgtatggtt ttaccgggat ctggctggca caggtcctgg catttacgcc
    20041 aatggctttt atgatcctcg atggggccat taaaaccatt catccctcgc tggaagaagc
    20101 ctcctacacc ctgcgcgcca gccgctggca aacctttagc ggtgtgtttg tgccgttgtt
    20161 aaagcccgcg cttgccaatg cctttttaat cgtcgtggtg cagtcactgg ctgacttcag
    20221 taacccgctg gtgcttggcg gcaacttcga cgtactggcg acgcaaattt acttctacat
    20281 taccggctcg cagctggatt atcaggccgc cagcaccctt ggcgctttcc tgctgctgtt
    20341 ctcgctactg gtgttctgcg tccagtatat gtggattggt aaacgctctt acgtcacggt
    20401 ttccggaaag tcctaccgcg gcgatgtcca gccactaccg gtcaccctgg tgtgtagcgt
    20461 ggtcgccata cttgcgatat ggattgcctt taacgccctg ctctatggca gcattttcta
    20521 cggcagcttc accgtgaact ggggcgtgga ctacacccta acgctggata actttatcaa
    20581 gttatttggt caggggatga gcgacggcgc gtggccttca ctgttagata ccttgctcta
    20641 cgccgggatt gccgcaccca ttaccgctat ttttggcctg ctcatcgcct gggtggtggt
    20701 gcgccaacaa ttcaaaggca aaaagaccat cgagttcacc accatgttgt gctttgccgt
    20761 accgggtaca gtggcgggtg tctcgtatat tcttgccttt aatagcgccc cagtctatct
    20821 caccggaacg gcagcaattg tgattatctc gatggtgatg cgtaacgttc cggtgggtat
    20881 tcgcgccggg attgcaggtc tggggcagat cgacaaatcg ctggatgaag cctcacttag
    20941 cctgcgtgca ggttccctgc gaaccattac gcacatttta ctgccgttat tacgcccagc
    21001 tattctctcg gccctgattt acagctttgt gcgggctatc acgacggtca gcgccatcgt
    21061 ctttctcgtc acgccggaca cccgcgtggc aaccgcctac atccttaacc gcgtagaaga
    21121 cggcgaatat ggcgtagcca tcgcctacgg ttcgattctg atcgtggtga tgctggcgat
    21181 tattttcctc tttgactggc tgatcggtga agcacgtatt tcccgttcta aagccaaaaa
    21241 ccaggcgtaa tggagcgcac tatgagtcag aaaaattttg tggaactgcg caacgtcact
    21301 aaacgattcg gcagtaatat ggttatcgac aatatcaatc tcaccatccc acaagggcaa
    21361 atggtgacgc tgctcggtcc ttccggatgc ggcaaaacca ccattctgcg tctggttgcc
    21421 gggctggaaa aaccgagcga agggcaaatt tttatagatg gcgaagatgt cacgcatcgt
    21481 tccattcagc aacgcgatat ctgcatggtg tttcagtcat acgctctgtt cccacatatg
    21541 tcgctgggcg aaaacgttgg ctatgggctg aaaatgcttg gcgtgtcgcg taatgaagtg
    21601 aagcagcggg tgaaagatgc gctggcaatg gtggatctgg aaggattcga agaccgctat
    21661 gtcgatcaga tttccggcgg tcagcaacag cgtgtggctc tggcccgtgc gttgatcctc
    21721 aagccaaagg tgctgctgtt tgatgagccg ttaagtaacc tcgatgccaa cctgcgccgc
    21781 agtatgcgcg ataagatccg cgagctgcaa aagcagttta atatcacgtc gctctacgtg
    21841 actcacgatc aaagtgaagc tttcgcggtg tccgacactg tactggtgat gaataaaggt
    21901 cacatcatgc agatcggttc accgcaggag ctctatcgtc agcccgcctc ccgctttatg
    21961 gcaagtttta tgggcgacgc caacctgttc ccggcgaact ttagcgaaga gtatgtcgat
    22021 atctacggtt atcgcctgcc gcgcgcggcg tatttcccgg tgcaaggtag aggcaccgtc
    22081 ggcgttcgcc cggaagccat caccttaagc aatcacggcg aagagagtca gcgttgtgtt
    22141 attcgccatg tcgcttacat ggggccgcag tacgaagtga ccgtagaatg gcatggtcag
    22201 gagattttat tgcaagtcaa cgccactcgt ttacagcccg atactggtga gcactattac
    22261 ctcgaaatcc atccttacgg gatgtttgtt ttagcggatt cggcataaaa atagcgcata
    22321 aaaggtcccc tccagctcag ctgtctctta tacacaaata accagccgga gatttgtgat
    22381 gtctgtcagc ctgctttagg ggggactctt tccatccggt cacccgcagc cattttttga
    22441 acttttcttc acccgttgct cttctcttat atttcatttt cttgtaagtg ccccatcagc
    22501 gacgtcttgt gaaaattgtc ctgtctggca acaatcgcgc ccatctatat tgatgggctc
    22561 gaacgatgaa ctctcaaaca acactagata tcaccggctt tcgcacttat tttccagacg
    22621 cattacgtct cagatttgac gagcaattat ctgtccgggc aatcgcacag cagttaagac
    22681 tcagttattc ccccattcac aatctctttc aacgaattaa cgcctcaggt attgtctgac
    22741 tatgacccga gtccctttca ttcgcccaac cgataccata ctttatgcca acagaaagaa
    22801 tgaatgaacc gcgcctaaaa tcagtgaaga cacatggcac aaagcgcgac atgtctcaca
    22861 tttaacccct gaggggggca acaagtataa aaccgttttt tgattaagtg gtcggatatg
    22921 aaaaagggaa ggtttatgtg aaaaatacct gtgttttctt tgaaaacaca caacagtatc
    22981 aggttattta tgtttataca gggcgttcca aactgacctg aataattacg caagttgcaa
    23041 caataaaaac atttttgccg tagtgtgagg tactccggta aattgctttc caggtgcaga
    23101 caatgactaa cgaagaacta caggcaatcg cattatccat gcttgagcgc cagcgcgaaa
    23161 aagtacacca gcaacgagag tctttgctta acccatctcc attcaccagg ccaaaacgat
    23221 tggagcttga agcattcttg gacaaatacc cacggcggct taaatcaggc aaaaccagac
    23281 caccaggctg atagctcaca gtttgttctc ctattcaata aagcggataa gaaccatcag
    23341 cgcggtagcg taaggcatta ctgcgcattg ctttacgagt aataacaatg gatttatcac
    23401 cagaagacgc aaagttttta cgagaactgc aacaccgtat gctctgagaa gccattatcg
    23461 aacagcaaac gggcatgagc gccatagacc cattgcgata aaaacgggcc tcatctcctt
    23521 tagttgacga caattccaat gcggataaaa cacgtccacc acactgatta catttttcat
    23581 tagcacagtg gtgtaatgca gcatacgagg aaaaccggat gaatgagaac agaataaaaa
    23641 ggctggagca actgttacag gcacgccaac gggagtttgc cactaaatgg ggacgtgccg
    23701 cgttgccaca tgaacgccta acctattttc cacttgaaga cctcattaaa aataatggca
    23761 aaaaaatcag gcctgataag aaacactttt tagaatgcaa tgaagacaaa taacaacaat
    23821 ctgcatattg ccaccaacca cagatgtggc atcgttatca gcgcgcttga ttttctttat
    23881 atccactgac gagcttaccg gggcaccttt gtttgccggt cattctggta tcatgcagat
    23941 atttacggcg tacaagccca ctagcatgac attgtaataa ggcctgagcc taacgcttta
    24001 atgtgtgtca tacactcagt ggcgtaatcg ttgacaatat ggtgcatgta atcgtaagtg
    24061 tccagcgaaa tccatctggt cataatctga agcatccgac aaagtggtgt ccaccaaata
    24121 agtgatctgc gcccaatatc ctgagccgga actttgacga aggccaacga aaaatggggg
    24181 cggatattac agagttctcg gtacagggga aaagtggtac ctgtcgccag ttctccgatc
    24241 tgtttaaccg ggaaattatc tcctacagct tgtcagaaag gtcggtgatg gagcatcgtt
    24301 aatacgattc tgtacgatgc attcacagtg cccggccaga ggatgccccg tcgctgcata
    24361 cggatcaggg ttggcaatat cgaatcgcag gctataggca aagttaaaag ccccatggag
    24421 tagcacaaaa tatgccgcac aaaggaaacg gtctgaataa cgcagtgatg gagaacttct
    24481 tcggcacaca gctaaagcgc atagtgatcg agcgctgatt ctggcgaaca actgaactaa
    24541 cacatcagaa tctgcattat gttaaataaa tatgaaaaga tggtttaaat accccgttac
    24601 ttttgactta cactatacgg tatcgcatcg tttaatacgt ttaatattcg caccggccag
    24661 atttttattt ctattagttg tcacaatact gaatgcgtac gaccacagta ttctggctcc
    24721 tgtgtggtca tgctttaatt ctgcgttccg ggcagataag cagttgcttg caggaatcct
    24781 tcttgtgtta atatcagttc ccctttttac cagtgctgat ttccacattc cgtccaacag
    24841 agcttatagc ctttccctgg attatagcat tgtccggctg aagttctttt tgaataataa
    24901 tagaagcact gctggcagat ccagtccgtt tttcataacc aactgtactg ataaccataa
    24961 tctaatcagt agaaattgag tcgaaaataa gcactactcc atacaggata attagaggtc
    25021 agtttgatta ttcacaattc atcatcagca ttttctgtt
//
LOCUS       DBANUU010000067        24883 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000067 DBANUU010000000
VERSION     DBANUU010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.067"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            27..947
                     /locus_tag="ACIE68_002634"
     CDS             27..947
                     /locus_tag="ACIE68_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFX5908930.1"
                     /translation="MPEQKMKITEEAFSDFTGHMCRAGFTNSISDEPLTERQQSQLSA
                     CLQAVPLSQSVNITPASPSVLVGKTVQLSAGISMGKSASSFTWKSANDQIATVNGTGL
                     VTGVAPGKVKITATDQETQLSASVEVTVNPVAVQSVTVTPDSTSVEKGKSVSLKANVQ
                     PSNATNKAVTWSSKNEDKATVDQSGNVTGVEVGTATIEIVSQDGSKKATATVEVTAPV
                     VAVTGVEIDPNSTTVEANKTVQLTANVEPAGATNKTVTWESKNTEFATVDSETGVVTG
                     VAAGTATIEVTTQDGSHKATATVEVTAAQE"
     gene            992..1426
                     /locus_tag="ACIE68_002635"
     CDS             992..1426
                     /locus_tag="ACIE68_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908931.1"
                     /translation="MKPAKIVLLEPQFSGYSGMLCGVQFENGVSVAELPFIDQQRICA
                     SMRASTVEGKNVSPSAAYSDRGELTADLITEPAAPDIVPMKRGTPDEPAKQIQTFTRE
                     ELESIADNEGIAGLRVIGNQVGVKAKGIVEMIEGILKAQGGE"
     gene            1426..2259
                     /locus_tag="ACIE68_002636"
     CDS             1426..2259
                     /locus_tag="ACIE68_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908932.1"
                     /translation="MAQIDSYRSGEAVSLSFAFNVLDIESATYTVKDSTGAILVDGEP
                     LEITSGQMSIPVVVSAEYNQLSEKERDLRYVIVKAVASGLTHEERQMYVLLNSFELSI
                     PEQSFATVADAQMQAIDMLNGDTLLSDGEGLMRKRLIEATRRIKTLPFSIRKILRIDF
                     DRYDRPQNMLNVYDIPWGADGAYRHDLVDWEKMTQEKFEEFPDYFKEALMLAVVNEAC
                     EIANGNDVAAAREDGILSESIGETTNMYRTGKAANVHVARSTWRLLVSYINNRMIVRR
                     A"
     gene            2339..2683
                     /locus_tag="ACIE68_002637"
     CDS             2339..2683
                     /locus_tag="ACIE68_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908933.1"
                     /translation="MNISWQTEIAIYRFGAKNVYGEAQLQFVRKTNVGVVKFEQSNEK
                     SSVRADSSGSRGKASLELFDAVLVVPLEAAVQLDDVLILEGQKLKVSSVHRRWGLRGR
                     PGHLEVGANIWV"
     gene            2674..3147
                     /locus_tag="ACIE68_002638"
     CDS             2674..3147
                     /locus_tag="ACIE68_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908934.1"
                     /translation="MGLKYDAHQFKRAGNRLNNSQKAFKRYLIRDMEKLARLVERLSR
                     AMAPLETGSLETAIFARVIKEGYSGLRIELSVSGAKPRQGHPGVEVGDYAKYMELGKY
                     RLGYLSRMKNVTNPPIAGVKPRVGPHFLERAVEISEKQFSEAILEAARKAGFTRG"
     gene            3149..3532
                     /locus_tag="ACIE68_002639"
     CDS             3149..3532
                     /locus_tag="ACIE68_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFX5908935.1"
                     /translation="MFIEAFASLMQKAKIGTVGTDIFCHYLPANVKSGVLLINPNTGI
                     SIDHELQGFYHESFTIIVRGSSITTTVEKANKIIEMFPVEETESGGVYFRLVRPMAMP
                     IIYPKNDGALIEAGIPVEFAGYLLN"
     gene            3607..4353
                     /locus_tag="ACIE68_002640"
     CDS             3607..4353
                     /locus_tag="ACIE68_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFX5908936.1"
                     /translation="MSNTHVKNIKLGACKVSFGGVDLGYTKGGVQVEVATETLKVTVD
                     QLGQTVISELVQGRNITITAPLAESVLQNMVDLMPGSTLSEDDNSVTITSAQGVNLID
                     VAKELVLTPQDTTDYVLTIPKAATAGNFTMTYQSDDVRVFSVQFSAYPDDEGVLGKMS
                     GPKPVKTVSISPESPTVKAGETVQLTAEITPADAADKTGVWESEDQEKATVDQTGLVR
                     GVAQGSTNISFTSNSGGKKATKSVTINPAD"
     gene            4409..4726
                     /locus_tag="ACIE68_002641"
     CDS             4409..4726
                     /locus_tag="ACIE68_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908937.1"
                     /translation="MTKLLDLDSILPPKKSIKFGGQEYPIVEMTVGLFVSIKQMEGKD
                     LMNMSPVEQVTAYADLVRKVIPSVPDEVLEKLTVPQLQQIFTFAMEVIDEENEKAAGE
                     GAK"
     gene            4852..5076
                     /locus_tag="ACIE68_002642"
     CDS             4852..5076
                     /locus_tag="ACIE68_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908938.1"
                     /translation="MLSRNIDRLRAEEDVRNLQVARASQADGEAVKAFMEGLQLRIGR
                     PVVTDKVYDPSQDKADPDAKEQLMQIFGRG"
     gene            5084..9655
                     /locus_tag="ACIE68_002643"
     CDS             5084..9655
                     /locus_tag="ACIE68_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFX5908939.1"
                     /translation="MSQNVEFILSLEDKQFTASIDRAGKLLTRFGEQVTKPAQKIQTF
                     ERSLGSVARIIGVLESKLDSTADKLQDVAAGFELASDATRKMRGNITSLNSGLKALIE
                     RVDTTTSSVDKLTASLRKVQSELNDFSDWATYASKSASRFGTEVKEASVSVSGMNTRL
                     NTTTKRLSNWGVTTSQAAEGLKKVREQMDEVIGRQQLISKPVRVRTSGSGDGGSGRRS
                     GASGHSGKSNEGGMFSGLRGNIFLLGEIGDAARTVTDIMFGWQKPIVEAAAEMERMRV
                     MLRGLNKEKSNPGQAAADDMKYIVDMAQNAPFAMQALTDSFVKFRSAGLDPTDGSLKA
                     LVDSVARFGGDSELLKRAAVAVQQMSGKGVVSMEELRQQLGEAVPNAMQAMADAAGIT
                     MGELTKAVASGTVEAKQALSLMFVGLRAENENAAKDMMQTYTGALAQLQTSFTLFADR
                     VGQAGYLDSLSKGMKELASIMNSAEGISFANSLGSGLTTAIDGLRQLAQWLAKNQELV
                     INLGKVVAAMVAFKLMRAGIMGVVGAGSQMVSTFATMATAIQTPFNLGATAVTRFNRA
                     ARMGLAPIPSLIFAIRGAITGLKGAFAGLTAFIAANPIGAAFTVATVAVAGLITYMTM
                     LRSETSKVVDEIRKIPEAMTAAKRAQMADRAEKLEKQILADQRALKTGEGVNYVSNSA
                     GVITHKESKADIEARLKKNQEEYQKITGTIALGDGAVAKRLAKEAAESQIEKIRAENQ
                     IFAATFVKARQEALDKIQKINDDGSLSDDEKNKLLAPLRETVNKSYLEPAQKLVDSLS
                     SRKNATEKQIATLNDQLEKAKKDGNTEQIQKLQGSIRGYQEHLEAVAQELTQAEFERD
                     NAAKTGKGVMSNQGTVLGLGTTDKAAQKALAQYMRNQMDSATYQRTLPDGTPMMDFEG
                     KPIIGPKQLKTQLNLQKASSASSLEKMSDEERAAAIAALTKAREQDAAAAEKAAQRSA
                     NASQRAAKKEQAAQQKLAAGYQKALDKADQLMGQMGESSKATVSFDQSLRDTTKSLTD
                     LANAVPNEFITQEMIDKAKKRLEDLKNATPEYREMFNRRNVEQMISTWAPEADSIISA
                     GYTPSREEKVADFEDTYNRNLKALIELRDKASDPKVVALYTKKINQLIAASNTALIKE
                     TGTATQKLALEYENLAEQIESTWTDLFSGLTDVLTDFVINGKMSFSSLSQSILKDITN
                     MVVKSQITLPLMNMLGMGTTAAGSSQSGNLLTGVASAVANQGVRMGNTVNGDKSVGEA
                     TKETSSSVTGLGQTTQQTTSAIGTATNAIGSWVSGLFDSTEAKDAETKAVKDSIFSMQ
                     NLSSVTGALSAAFAMLGANASGSGNKWLNFGATVASGLVSVWAGGGFDGLMSGSSGST
                     SGFNNLTSSAADGTNGIPAIPKFANGGIFGKDGVIPLRAYQKGGIANSPQLALFGEGD
                     MNEAYVPLPDGRTIPVTLSTDGMSGGGNVLSPVSIEINVHSDGSTTESGDTESIWNNA
                     AQRMKAIALETIAQEKRPGGSLNPNTQRN"
     gene            9698..10033
                     /locus_tag="ACIE68_002644"
     CDS             9698..10033
                     /locus_tag="ACIE68_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFX5908940.1"
                     /translation="MERQTFNWYPDYESEKSVKPNVTVLNFGDDYEQRQAQGLNRIKE
                     EWSLTFTRSYNEINAIDDFLTERAGVESFYWVNPRGKRIVVVCDSHTVKRYQGYCVLT
                     ATFRQVFEA"
     gene            10116..10814
                     /locus_tag="ACIE68_002645"
     CDS             10116..10814
                     /locus_tag="ACIE68_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFX5908941.1"
                     /translation="MGIKADIQSLSPSALIELFVLDMSNTTSGGKLFFHAGTNELMQP
                     VVWQGVTYEPWPIKASGFDKTGQGTLPRPKIQVSNFAGTVSAEVQANDDLVGCRIIRK
                     MTLARFLDAVNFKDGNPTADPNQHFPDEMWFIEQKTLETHQVVEFELSSVFDLMGVQL
                     PYRQIIKNTCPWKYRGPECGYTGPYFDKNNQQTSMSGADYCTKRYDACNARRNYFADG
                     VIHFGGFIGATRYG"
     gene            10807..11604
                     /locus_tag="ACIE68_002646"
     CDS             10807..11604
                     /locus_tag="ACIE68_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFX5908942.1"
                     /translation="MGNKAIPELGSDVMQQIYLCAINRYPNEACGFLVRTNGDKYRFM
                     EARNVSENPQNTFVMHVDDIMAAEDAGDVIAIWHSHTDESAEASDADRAGCEATEVPW
                     MILAIRKNVEGDAPFHFSEMNVITPDGFEMPYLGRPYVFGVFDCWMLCRDYLKREFNV
                     ELNPNAHLHIPSWYTGDNDILDQNYRNEGLVRLAPGTEPQRGDIFFIQYGKMPDHCAV
                     YIGDGMILHHQIDRLSCRAYYGGMYQKHTTHHLRHRDLLKGDETCLS"
     gene            11592..12185
                     /locus_tag="ACIE68_002647"
     CDS             11592..12185
                     /locus_tag="ACIE68_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFX5908943.1"
                     /translation="MSELVHVQLGGPMAKHFGRHWHLKVRNTKQALDLIEANKPGFKA
                     WMKRNIKTYDRYHIQITNKQGHKWSVDESEYQMMGQSDNIAKIRITPVPRGSGGSAFG
                     WFQTVVGAALLVVSAVVMPALAPLGLSLMMGGISQIISPQATNESVRQADNSNSYYFD
                     GPQNTENQGNPVQLIYGEEILVGSQVVSSSITIDQLM"
     gene            12203..16930
                     /locus_tag="ACIE68_002648"
     CDS             12203..16930
                     /locus_tag="ACIE68_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFX5908944.1"
                     /translation="MEQFKKKKLPLFIAGAGGKKSSKSSSRTPVEADDTVNSRAMAAI
                     LDLLGEGVIGGLVNGARSIFIDDLPIVNEDGSSNFSGITWDFRDGSQDQTPMSGFDFV
                     ETPKSVNIQLKKTHYVTVSIDNDEADRVRVIMKFPSLRSIDKKTGDTNGTTVEYKFQI
                     ANGDSTFVDVVAEGEKSVGIKLTAKKTGVYYRSYELKLPKPGRAYKVRVVRITDDNSS
                     QYLYNDTWVDSIGEIVDTPMNYPNSVLVGLKVNSEQFGSTMPSRSYLVRGLKIRVPSN
                     YNESSNTYAGVWDGTFKLLSSSNPAWILFDLLTNARYGLGQYVSESMIDLGQLYQIGR
                     YCDEEVDDGFGGKEKRFAINTQITSRQDAYRLIQDIAGAFRGMVFWAGGMVNIMQDSP
                     SDPVMMFTNSNVKDGLFTYKGSARKDRPSVALVTYNNKEDGYKQNIEYVEDQDAMRRY
                     GERKTEVVAFGCTSRGQAHRVGLWLLYTARMESDVITFTAGLDASFLMPGETVLIQNK
                     YRAGKRNSGRIVAFTKNSVTLDAPVSLAKGGCFIRILNQEGKIVERDVLETGENITKV
                     TFSKALSSAETPVLNGVWTITEPDLEPMRVRIVNIAQGETPGSFDITAVENNPSKYEA
                     IDNGATLIPQNTTVLDPTYSKPSNLQITEGTYLSSPGNLSVKLTATWEGKSPEYWISW
                     RRSDENNVSNWQSARVTEEQYEIVNVAENGRYDFQLYAVSFNGKKTEIISTVYQVLGT
                     MTPPDAPTSLTAVGDYRNVILNWVNPDSVDLDHINVYASQTNNLDTAKLIAESASTTF
                     THAGLGDSETWYYWVRASNKRGMLSPPNSNLGTEATTRDVLSFLTGKITSSELGQALL
                     EDINSKASQEAVDELNEHINQSVESLEGAVNDVKEDIAELDKQFSDNLADFEIKFNER
                     SDALENAQTELKGEVSATIDKVNEAFEKIDATDAAIVEIENTVSEHDKALANTVEAIK
                     AARDEAAALIAKESEARVEGDAANAKQLEVLQSTVEESSAAVEEMKKTVAEVDRASAE
                     LTTNIEALAKTNIDLALRQDEDQHKQMVNNAKIATTQKTFADDMSAMATKVEEIRVEI
                     GEDIKASILEESTARADGDEALAKRVTQLQSKFEGDISAAISTEQEARTSADEALTKQ
                     ITQLESKVSNDIVAAIKEEQEARATEDSALASQITQLQAKVDDDISAAIRSEQEARAS
                     GDSALAKQINQLQAKVDGDISAAITQEQEARASADEALSREINSLRAQTGTDIAAAVA
                     VETKARTDADSALSTQITSLTAKANDLEASLARETTARADGDTALTKEVSSLKAQTAK
                     DISAAVAVETQARTDADSALSSQITKLTSQYKEDIKAAVATETKTRTEQDAALATQIT
                     NLESQTAANISAAVTTETTARTQADNALSGRIDTLKAEVDGNTAIIQQQATAIADTNK
                     KVSTAWTLKMETSTSGGQKYVAGIALGIDTTGLSQFLVQADRFGLVNSVNGKITTPFV
                     IENSIAYMNGAYIKDGTITNAKVGDLQSTNFVSGRSGWRFGKNGTLEINGNSGGNGRL
                     VINGQRIDVYDDNNVLRVRIGRL"
     gene            complement(17566..18249)
                     /locus_tag="ACIE68_002649"
     CDS             complement(17566..18249)
                     /locus_tag="ACIE68_002649"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908945.1"
                     /translation="MALPFAADACLVASSFEADADDADDAAELADDAAAVFEDSALVS
                     DAFAFVSDVFAAEADLAAELACSVASPAFVVAVEADDAALSADFPAAVALAEACPALV
                     DAALADDAAAVFEPAAAEALCPAAVSEDLAFVSDVFAAFAEFPAAVAEEAALLALKDA
                     FVSDDFAASFEAAASRAEVAASDAFVVAVDAEVAADCCDAAAFVSDFFAAVALALAAL
                     CEDAAADAL"
     gene            18386..21052
                     /locus_tag="ACIE68_002650"
     CDS             18386..21052
                     /locus_tag="ACIE68_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFX5908946.1"
                     /translation="MEDASLTTKGVVKLSSAVDSTSESLAATPKAVKAANDNANSRVP
                     SNRKVNGKALTADITLTPKDIGTLNSVTMSFSGGAGWFKLATVTMPQASSIVYIALIG
                     GAGYNVGSPHQAGISELVLRAGNGNPKGITGALWKRTAVGLTNFAWINTSGDTYDIYV
                     EIGNYATSVNIHWDCTANATVSIYTSPTYSASKPSSVTDGVVYTMYSTHQKPTPLDIG
                     ALPTTGGTVSGPLSVTGGITGTLNGNASTATKLQTARSIGGVGFDGSANINLPGVNTT
                     GNQNTTGNAATATKLQTARTIGGVSFDGTANINLPGVNTTGNQNTTGNAATATKLQTA
                     RTINGVSFDGSANISLSPANIGCPASPTGWLTTGSNGGAITTAQLVTLLQNNGAFNTK
                     SWIARCAWAYANSATIPNSETGCGVIPLAGAVIEVFNNGSSSNNYTIRITTATTTSVS
                     GALTNAEFIYVFNGTDYSPGWRRVYNTKNKPTASDVGALPLTGGTLSGGLTSSGEIIS
                     KYANGFRIAYGSFGFFIRNDGSNTYFMLTASGDTLGSWNGLRPITINNTSGAVSIGNG
                     LNVTGGVNGSLNGNASTATKLQTARNINGVKFDGSGDININTLVSRGRVTALSGSAQG
                     TAGIQMYEAYSNSYPTTYGNVLHMKGASAAGEGELLIGWSGTSGAHAPVFIRSRRDTT
                     DAAWSAWAQVYTAKDSIPGVNTTGNQNTTGNAATATKLQTARKIAGVAFDGSADITLT
                     AANLNAYTKTEVTNLLSSYVKSSALPSMTVRTSSVSGGDMGMSLSTFISHLKSNGAFS
                     KSYWIGFGDAMGFNAGSINNITGFGAVELAESIIEVFNLPNGDYTIRLTTSHKADYGG
                     VTNAILVYHYRSNRSPSGQWLKFAGTVGATSN"
     gene            21255..21578
                     /locus_tag="ACIE68_002651"
     CDS             21255..21578
                     /locus_tag="ACIE68_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908947.1"
                     /translation="MSNEMAGVTTEQVERIAAIVAREVVGKLGKELREEIGQEVNDQL
                     KTYFGDMTPAQHSIQHSNLDKLLNRLDSISSGFFGGIVSKITSFIITALLLGLAAYGV
                     KSGLQ"
     gene            21592..22284
                     /locus_tag="ACIE68_002652"
     CDS             21592..22284
                     /locus_tag="ACIE68_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908948.1"
                     /translation="MKTPRGIRNNNPGNLDKGSPWQGLVANPDEPRFCTFKDPVWGIR
                     ALAVTLITYHDKRRAKDGTSIDTIREVIERWAPPNENNTDAYINEVSKAVGVTADMII
                     DLHDYDILRPLVEAIIRHENGRGPLKTLNTWYAAEVIEEGLRRAGVVKPVKTVKAVPV
                     TKETAGATVTAGIGLAQLADVMPQVSAAMDKAQGHISSGDTVRIIFGIATIVVAGFIA
                     WSQVRKHQKGMV"
     gene            22286..22537
                     /locus_tag="ACIE68_002653"
     CDS             22286..22537
                     /locus_tag="ACIE68_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908949.1"
                     /translation="MLGSLMTKLKVALITLAAVLFVLVGAYTMGGNAARRAMEEKAKQ
                     EDRKRLQNTVKVVNETSSKIRQKDASAVHRELYDKWVRH"
     gene            complement(22706..23227)
                     /locus_tag="ACIE68_002654"
     CDS             complement(22706..23227)
                     /locus_tag="ACIE68_002654"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFX5908950.1"
                     /translation="MSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKIL
                     RAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQKKVPYRNVPSVTLGRLALDKSL
                     QGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
                     FYPTKSIEKLFEE"
     gene            complement(23235..23504)
                     /locus_tag="ACIE68_002655"
     CDS             complement(23235..23504)
                     /locus_tag="ACIE68_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004109769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFX5908951.1"
                     /translation="MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERA
                     VKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKRAADRLKSME"
     gene            23823..24491
                     /locus_tag="ACIE68_002656"
     CDS             23823..24491
                     /locus_tag="ACIE68_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFX5908952.1"
                     /translation="MTKIFVVGGTKGGPGKSTVAQQIAVCLKVKKKKKVYITDIDIQR
                     TTTSWCEDRRQNEDLELIPFAYVQDDIIKHLKSLQGRAEFVVVDAGGFDSEIQRQAML
                     MADVIIIPLRPKRRDLKSLRDIDPIIDNVRNVNDKVKVRAVMNQCPALPSQVSRILAA
                     KEIVETFGIESAPVNLYNRNVYDDAEESGRSIFEMTGSERDKKAEAEFEEFVDYLLSL
                     EEEE"
     gene            24497..24850
                     /locus_tag="ACIE68_002657"
     CDS             24497..24850
                     /locus_tag="ACIE68_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5908953.1"
                     /translation="MKMGDLAKRKEPDAPAKNTTPLRQPVRPQGRPTRGKEKIKSRTM
                     SLEDEYFELLEMMKFIPRFEKFTRSDVIRAAIFHLAEKSPQEIEDIVKLNEAITAADV
                     TMRTDEIKRELMKKG"
BASE COUNT         7004 a         5472 c         6440 g         5967 t
ORIGIN      
        1 gcccaccctt tttgtaggag cgagaaatgc cagaacaaaa gatgaagatt acggaagagg
       61 cattttcgga ttttacgggg catatgtgcc gtgccggatt taccaattct atctccgatg
      121 aacctttaac cgagcggcaa caaagtcagc tatctgcttg tttgcaggcg gttcccctct
      181 cccaatccgt aaacataacc ccggcttcac cgtcagtttt ggttgggaaa actgttcaac
      241 ttagtgcagg tattagtatg ggtaagagtg ccagttcatt cacctggaag tcagcaaatg
      301 accaaattgc aaccgttaac ggcactggat tagtaactgg cgtagcacca ggcaaagtaa
      361 aaattactgc aactgatcag gaaacccagc tttccgcgtc agtggaagtc accgtaaatc
      421 cggtagccgt tcaatccgta acggtaacgc cagactcaac ctctgttgag aaagggaaat
      481 cagtcagtct gaaagcgaat gttcagccgt caaatgcgac gaataaggct gttacctggt
      541 cttcaaaaaa tgaagataag gcaacggtag accagagcgg gaacgttact ggtgtagaag
      601 ttggcactgc gacgattgag atcgtttccc aagatggcag caaaaaggca actgcaacgg
      661 tggaagtaac tgcgcctgtt gtggctgtta ccggcgtcga aatcgaccca aatagcacaa
      721 ccgttgaggc aaacaaaact gttcagctga ccgcaaatgt cgagccggca ggagcaacaa
      781 ataaaaccgt tacttgggaa tccaaaaata ccgagtttgc aacggttgat agcgaaaccg
      841 gtgttgtaac tggcgttgcg gctggtactg ccacaatcga ggttactacc caagatggta
      901 gccacaaagc gacagcgacc gtagaagtca ctgctgcaca ggaataatca atattgggcg
      961 gcgaaagccg cccatgtaga gagaatgcgt tatgaaacca gcaaaaattg ttttattaga
     1021 accgcaattt tccggttatt cgggaatgtt gtgcggcgtt cagttcgaga acggggtgtc
     1081 cgtagcggag ttgcctttta tcgatcagca aaggatttgt gcctcaatgc gagcatcaac
     1141 agtcgaaggc aaaaatgttt ctccgtctgc cgcatacagt gatcgtggcg agttgaccgc
     1201 agacctgatt accgagccag cagcacctga cattgtgcca atgaaacgtg ggacaccaga
     1261 tgaaccggct aaacagatcc agactttcac acgagaagag ttggagtcaa ttgcagacaa
     1321 tgaaggcatt gccggtctac gtgtgattgg taatcaggtt ggtgtcaaag cgaaaggaat
     1381 tgtcgagatg attgaaggca tcctgaaagc acaaggcggt gagtaatggc gcagatcgac
     1441 tcgtatcgta gcggtgaagc tgtttccctt tcatttgcct tcaacgtgct ggatatcgaa
     1501 tctgctacct acacagtaaa ggacagcacc ggggctattc tcgttgatgg cgagccgcta
     1561 gaaattacca gcggtcaaat gtcgattccg gttgtcgtgt cggctgaata taaccagctc
     1621 tctgagaaag aacgcgatct gcggtacgtc attgtgaagg ctgttgcatc gggtctgacg
     1681 catgaagagc ggcaaatgta tgttctgctg aatagttttg aactgtcgat accagaacag
     1741 tcgtttgcaa ctgtcgctga tgctcaaatg caggcgatcg atatgctgaa tggggacact
     1801 ttgctgtcgg atggtgaagg cttaatgcgc aagcgtctca ttgaggctac cagacgaatt
     1861 aaaactttac cgttctcaat ccgcaaaatt ctacgtatcg actttgaccg atacgatcgc
     1921 cctcaaaata tgctgaatgt gtatgacatt ccgtggggag cagatggagc gtaccggcat
     1981 gatttagtcg attgggaaaa gatgacgcag gagaagtttg aagagttccc tgactacttc
     2041 aaagaagcgt tgatgcttgc cgttgtcaac gaagcgtgtg agatcgcaaa tggcaatgat
     2101 gttgccgcag cacgagagga tggcattttg tcagagtcaa tcggtgaaac aaccaacatg
     2161 taccgtaccg gtaaagctgc aaacgtgcat gtggctcgca gtacctggcg cttgctggtc
     2221 agttacatca acaaccgtat gattgttcgc cgtgcgtaac gccagtcgca ttatttactt
     2281 ttggtcgaaa gggtttttgg ccagtaatga gagcgaccga caacaacaga gagagagcat
     2341 gaatatttcg tggcaaacag aaattgcgat ctaccgcttt ggcgcaaaga acgtttacgg
     2401 cgaggcgcaa ttacaattcg tcaggaagac gaatgtcggc gtggttaagt tcgaacagag
     2461 taatgagaaa tcgtcagttc gtgctgatag ctctggtagt cgtggtaagg cgagtctgga
     2521 gctgtttgat gctgtgttag ttgtcccttt agaagcggct gtacaacttg atgatgtgct
     2581 gatccttgaa ggtcaaaaat taaaggtgtc cagtgttcat cgccgctggg ggctacgagg
     2641 aagacctggg catcttgaag tgggggcgaa tatatgggtc tgaaatacga tgcgcatcag
     2701 ttcaaacgtg cggggaatag actcaataac agccagaaag catttaagcg atatcttatt
     2761 cgggacatgg aaaagctggc gcgtttggtt gagcgtctca gccgtgcaat ggctccgctg
     2821 gaaaccggat ctctggagac agccatcttt gccagggtga ttaaagaagg ttattcaggg
     2881 ctgcgcattg agctttctgt gtctggagca aaaccacgtc aggggcatcc gggcgtagaa
     2941 gtcggtgact atgctaagta catggagctg ggtaaatacc gactcggtta tctttctcga
     3001 atgaagaacg tgacaaaccc accaattgcc ggtgttaaac ctcgtgttgg gccacacttt
     3061 ctggaaagag cggtggaaat tagcgagaag cagttctcag aagcgattct tgaggctgcc
     3121 agaaaagccg gatttacgag aggttaatgt gtttattgaa gcgtttgcaa gtttgatgca
     3181 gaaggcaaag attggcacgg tcgggactga cattttctgt cactacttgc ctgccaatgt
     3241 gaaatctggc gtcctgctta ttaacccaaa taccggtatc agcatcgatc atgagctaca
     3301 gggcttttat cacgaatcat tcacaatcat tgtgcgtggt tcgtcaatta ctacgacggt
     3361 tgagaaagcc aataaaatca ttgagatgtt tccagtggag gaaacggaat ctggtggtgt
     3421 ttattttcga cttgtacgac cgatggcgat gccaatcatt tatcccaaaa atgatggggc
     3481 attaatagaa gcaggcattc ctgttgaatt tgctggctat ttattgaatt aacttaatta
     3541 gtaagtatat acttaccatt agcgcaatga atgcgtagaa ttaacggaaa aaggagtttt
     3601 ccatcaatgt ctaataccca tgtaaaaaac atcaaacttg gtgcctgtaa ggtgtcgttt
     3661 ggtggtgtgg atttgggtta caccaaaggt ggtgttcagg ttgaagtagc cactgaaacg
     3721 ctgaaagtca ccgtcgatca gttggggcag accgtaattt ccgaattagt tcaggggcgc
     3781 aacattacca tcaccgcgcc tctggctgag tctgtactcc aaaacatggt tgacctgatg
     3841 cctggttcta ctctgagcga agatgacaac tctgtcacga tcacatctgc acagggcgtt
     3901 aacctgattg atgtggcgaa ggagctggtt ctgactccgc aggacaccac cgactatgtg
     3961 ttgaccatcc cgaaagcggc aaccgcgggc aacttcacta tgacctacca gtctgatgat
     4021 gttcgtgtgt tctctgttca gttcagcgca tacccggatg atgaaggcgt tctgggcaaa
     4081 atgagcggtc ctaaaccggt aaaaactgtg tcgatctcac cggaatctcc aaccgtaaaa
     4141 gctggagaaa ctgtgcaact gacagccgaa atcactccag ctgatgccgc agacaaaacc
     4201 ggtgtttggg aatccgaaga ccaggaaaag gccacggtag atcaaacggg tctggtacgt
     4261 ggtgtggcgc agggttctac aaatatctcc ttcaccagca atagcggtgg taagaaggca
     4321 actaaatcag taacgattaa ccccgcagat taatcagtaa ttaagcagag gctcaggatg
     4381 ggcctctctt ttaaaaggat tttaaccaat gaccaaatta ctcgatctag attccattct
     4441 gcctccgaag aaaagcatca aatttggcgg tcaggaatat cccatcgttg aaatgacggt
     4501 aggtctgttc gtttccatca agcagatgga aggcaaagat ctcatgaaca tgtctcctgt
     4561 tgagcaagtg acagcttatg cagatctggt tcgtaaggtt atcccttcag tacctgacga
     4621 agttcttgaa aaactgactg ttccgcaact ccagcagatc ttcaccttcg ctatggaagt
     4681 gattgatgaa gaaaacgaaa aagcggctgg cgaaggggca aagtaatttc ccgcgatgaa
     4741 tccgggacaa ggaccgtctc aatagatctc ggattctatt tcagtcgtgt agttgctcac
     4801 tacgccgtgt cgccattaga gctactgaac gttcctctca cgatgttctg gatgctcagt
     4861 cgcaatatag accgtctgcg tgcggaagag gatgtccgca acttacaagt cgctcgtgcg
     4921 tcccaggcgg atggcgaggc tgtgaaggcg ttcatggagg gtttgcaact caggattgga
     4981 agaccagtcg taacagataa agtctacgat ccaagccagg ataaggcaga ccctgacgcc
     5041 aaagagcaac tgatgcaaat atttggcaga ggatgacaag ggaatgtcac aaaacgtaga
     5101 gtttatcctg tcgctggaag acaaacagtt tacagcgtca atcgatcgtg cggggaaact
     5161 gcttactcga tttggtgagc aggtaacaaa gcctgctcaa aaaatccaaa cctttgaacg
     5221 ctctttgggt tcggtcgccc gtatcattgg cgttctggaa agcaagctcg attctacggc
     5281 agataaacta caggatgtag ctgccggttt tgagcttgct tcagatgcta cgcgtaaaat
     5341 gcgtggcaac atcaccagcc tcaattctgg tcttaaagcc ctgattgagc gcgtcgatac
     5401 gacaacttct tcagtagata aactcaccgc gtcattgcgt aaagtgcaat cagagctaaa
     5461 tgatttctct gattgggcga cctatgcaag caaaagcgca agccgctttg gtacggaggt
     5521 caaagaagcc tctgtgtccg tgagtggcat gaatacgcgc cttaatacca cgacgaagcg
     5581 actcagtaat tggggtgtca caacgagtca ggctgccgag ggactgaaaa aggttcgtga
     5641 gcagatggac gaagttatcg gacgtcagca actgattagc aagccggtac gcgttcgtac
     5701 atctggaagt ggcgatggtg gtagcgggcg gcgtagcggc gcttctggcc atagcggtaa
     5761 aagtaacgaa ggaggtatgt tctctggcct tcgtggcaac attttcctgc ttggcgagat
     5821 cggagatgca gccagaacgg ttactgacat catgtttggg tggcagaagc ctattgttga
     5881 agctgcggcc gaaatggaac gtatgcgggt gatgcttcga gggttgaata aggagaagtc
     5941 caacccgggc caggctgccg ctgatgatat gaagtacatc gtagacatgg ctcaaaatgc
     6001 gccgttcgcg atgcaggcgt taacagactc cttcgtgaag tttcgttctg ctggcttaga
     6061 tccaaccgat ggttctctga aggcgctggt ggactccgtt gctcgttttg gtggtgatag
     6121 tgagctgttg aaacgtgcgg ctgtggccgt tcagcagatg tccggtaagg gcgttgtgtc
     6181 aatggaagag ctacgtcagc aattaggtga agccgttcct aacgcgatgc aggcaatggc
     6241 agacgccgca ggcatcacta tgggggaact gactaaagcc gttgccagcg gtacggttga
     6301 ggcaaaacag gcgttgtctc tgatgtttgt tggtctgcgt gcggagaatg agaacgcagc
     6361 gaaagacatg atgcaaacct acacaggtgc gctggcgcaa cttcagacgt cattcacgtt
     6421 atttgctgat cgggttggtc aggccggata tctggattct ctatcgaagg ggatgaaaga
     6481 actggcttca atcatgaata gcgccgaagg gatttcgttt gccaattctt taggttcggg
     6541 gttaacaacg gcaatcgatg ggttgcgtca gcttgctcaa tggttagcaa agaaccaaga
     6601 gctggtaatt aatctcggta aggtcgtggc cgcgatggtt gcgttcaaac tgatgcgagc
     6661 agggatcatg ggggtagttg gcgctgggag ccagatggtt agcacctttg ccacgatggc
     6721 gaccgccata cagactccat ttaacctcgg cgctacagca gtaactcgat tcaatcgtgc
     6781 ggcacgtatg gggctggctc cgatcccctc tcttattttc gccatccgtg gggcgattac
     6841 ggggcttaaa ggcgcttttg ctggattaac ggcgttcatt gcagcaaacc ctataggggc
     6901 agcatttaca gttgccactg tagctgttgc tggcctaatc acgtacatga ccatgctccg
     6961 cagcgaaact tcaaaggtcg ttgacgagat taggaaaata ccagaggcga tgacggcggc
     7021 caaacgtgca caaatggccg accgcgcaga aaagctcgaa aagcagattc ttgctgatca
     7081 acgagcatta aaaactggtg agggtgttaa ttacgtatct aactctgccg gtgttatcac
     7141 tcacaaagag tcgaaggctg acatcgaagc acgcctgaaa aaaaatcagg aagagtatca
     7201 aaagataacc ggcacgatag ctcttggtga cggcgctgta gcaaagcgtt tggctaaaga
     7261 ggctgccgaa tctcagattg agaaaattcg agcggaaaac cagatttttg cggcaacatt
     7321 cgtgaaagcc cggcaggagg ctctggacaa gatccagaaa atcaatgatg acggttcact
     7381 ttcagatgac gaaaagaaca agctattggc accgttacgt gaaacggtaa acaaaagcta
     7441 tctggagcct gcgcaaaaac tggttgattc actttcttct cgtaagaatg ccaccgagaa
     7501 gcaaatagcg actcttaatg atcagcttga aaaagccaaa aaggatggaa ataccgagca
     7561 aatccagaaa ctgcaaggca gtatccgtgg ttatcaggag catttggaag ccgttgctca
     7621 ggagctgact caggcagagt tcgagagaga taacgcggcc aaaactggta agggcgtaat
     7681 gtcaaaccag gggactgttc ttgggttagg tacaactgat aaagccgctc agaaggcgct
     7741 ggcgcaatat atgcgaaacc agatggattc tgcgacttat caacgtactt tgcctgacgg
     7801 cactccgatg atggacttcg aaggtaagcc gattattggg cctaaacaac tcaagacgca
     7861 gcttaatttg cagaaagcat ccagcgccag ctctctggag aaaatgagcg atgaagagcg
     7921 tgccgcagcc attgctgcac taactaaagc tcgtgaacag gatgcagcag ctgccgagaa
     7981 agcagcccaa cgatcagcta acgcctcgca gcgtgcggcc aagaaggaac aggcagcgca
     8041 acagaaactg gcagccggat accagaaggc tctggacaaa gccgatcagc ttatggggca
     8101 aatgggtgaa agctctaagg ctacggtatc gtttgatcag tctcttcgcg atacaacgaa
     8161 atcgctgaca gatttggcta atgcggttcc taacgagttc atcactcaag agatgatcga
     8221 caaggcgaaa aaacgtcttg aggacctcaa aaatgcgaca cctgaatatc gcgagatgtt
     8281 taatcgccgc aatgttgagc agatgatctc cacttgggca ccggaggcgg attccattat
     8341 tagtgctggc tatacgccgt ctcgcgaaga gaaagttgct gatttcgaag acacctacaa
     8401 ccgcaatctc aaagcgttga tagaacttcg tgataaggcg tctgatccta aagtcgtggc
     8461 gctttataca aagaaaatca atcaactgat tgctgctagc aataccgcgc ttattaaaga
     8521 gacggggact gcgacgcaga agttggcact ggaatacgaa aacttggcag agcagatcga
     8581 aagcacctgg actgatttgt ttagtggctt aactgatgtc ctgactgatt tcgttattaa
     8641 cgggaagatg agcttctcca gcctatctca gtccattttg aaagatatca ccaatatggt
     8701 cgtgaagtcg caaatcacgc tgcctctaat gaacatgttg gggatgggaa ccaccgcagc
     8761 tggtagttca cagagtggca atttgctcac tggagttgct tccgccgttg ccaatcaagg
     8821 ggtacgaatg ggcaacactg ttaacggcga caagtcggtg ggagaagcca cgaaggagac
     8881 gtccagttcg gtaactggat tggggcaaac aacacagcag accaccagcg caattggcac
     8941 tgcaacaaat gcgattggta gctgggtatc agggctattt gatagcaccg aagccaaaga
     9001 tgctgaaaca aaagcagtga aggactccat cttctcgatg cagaacctca gctctgttac
     9061 cggcgctctg tctgccgcgt ttgcaatgct tggagctaat gcttccggct ctggtaataa
     9121 gtggttgaat ttcggcgcga cagttgcatc tggtttggtt tcagtatggg ctggtggtgg
     9181 attcgatgga ttgatgtccg gttcttctgg ctcaacttcc gggttcaaca atctgaccag
     9241 ttctgctgcc gatggcacga atggtattcc tgcaattccg aagtttgcaa atggtggcat
     9301 attcggaaaa gacggcgtga tcccgctccg ggcataccag aaaggcggta tcgccaactc
     9361 acctcaattg gcgttgtttg gggaaggcga tatgaatgag gcgtatgttc cattgcctga
     9421 tggtcgaaca atccctgtaa cgctcagtac cgatggtatg agtggaggag gaaatgttct
     9481 ttctccggta tcaattgaga tcaacgtcca tagtgacggt agcacaactg aatccggcga
     9541 tacagaaagc atatggaaca atgccgctca acggatgaaa gcaatcgcgc ttgagactat
     9601 cgctcaagag aaacgccctg gcggatcact caacccaaac actcaacgta actaactatc
     9661 gactgccccg accggggcag tctcacaagg atgtgagatg gaaagacaaa cgtttaattg
     9721 gtatccagat tacgaatctg aaaaaagcgt aaaaccgaat gtaacggtac ttaattttgg
     9781 tgatgactac gagcagcgac aggctcaagg tcttaatcgt attaaagaag aatggtcgtt
     9841 aacctttacc agatcataca acgaaattaa tgcaatcgat gacttcctga ctgagcgagc
     9901 aggcgttgag tcgttctatt gggttaatcc aagaggaaaa cggattgtag ttgtatgtga
     9961 cagtcatacg gtcaagcgat atcaggggta ctgtgtctta acagctacat tcagacaagt
    10021 atttgaggct taagtatcta gataagtaag tactaattta ctatcattgt ggcgctgaca
    10081 ggatgttagc gcctacttat ttcaaggatg aaacaatggg aattaaagct gatattcaga
    10141 gcttatctcc ctctgcactc attgagttgt tcgtactgga tatgtcgaac acaacttcag
    10201 gggggaagct attcttccac gccggaacaa acgaactgat gcaaccggtc gtctggcaag
    10261 gagtgacata cgagccgtgg ccaatcaaag catcaggctt tgacaaaact ggccagggaa
    10321 cgttgccacg tccaaaaatt caggtatcga actttgccgg aactgtctct gcggaagttc
    10381 aggcaaacga cgatcttgtt ggctgccgca ttattcgcaa gatgacgctg gctcgcttcc
    10441 tcgatgccgt taattttaaa gacggcaacc caacagcaga tccaaaccaa catttcccgg
    10501 atgaaatgtg gtttatcgaa cagaaaactc tcgaaactca tcaggttgtc gagtttgaat
    10561 tgtccagtgt gttcgatttg atgggggtgc aactgccgta tcgtcagatc attaaaaaca
    10621 cctgcccgtg gaaataccga gggccagaat gcggctatac cggtccatat ttcgacaaaa
    10681 ataaccagca gacgtctatg tctggtgcgg attactgcac aaaacgttat gacgcctgta
    10741 atgcgcgtcg gaattatttt gccgacggtg tgatccattt tggcggattt attggagcta
    10801 cgcggtatgg gtaataaagc aatccctgag cttggctctg acgttatgca gcaaatctat
    10861 ctctgcgcca taaatcgcta ccctaatgaa gcgtgtggct ttctggttag aactaatggc
    10921 gacaaatatc gctttatgga agcgcggaat gtttcggaga acccgcagaa cacttttgta
    10981 atgcacgttg acgacattat ggcggcagag gatgcgggtg atgttatcgc aatctggcat
    11041 tcacatactg atgaatcagc agaagcatct gatgccgatc gtgcaggctg cgaagcgacg
    11101 gaagttccgt ggatgattct ggctattcgc aagaatgttg agggagatgc ccctttccat
    11161 tttagcgaga tgaatgtgat cacacctgat ggtttcgaaa tgccatacct gggcagaccg
    11221 tatgtatttg gcgtattcga ttgctggatg ttgtgtcggg actatttgaa gcgtgagttt
    11281 aacgtcgaac taaacccgaa cgcacacctg catattccat cgtggtacac cggcgataac
    11341 gacattctcg atcagaacta ccgtaacgag ggattggtgc gtcttgcgcc cggaacagag
    11401 cctcaacgtg gggacatctt ctttatccaa tacgggaaaa tgcccgacca ctgcgcggtt
    11461 tatatcggcg atggaatgat tcttcatcac cagatcgacc gccttagttg tcgtgcttat
    11521 tacggcggaa tgtatcagaa acatacgacg catcacttgc gtcacagaga cttgctcaag
    11581 ggagatgaga cgtgtctgag ttagttcatg tgcagcttgg cggccctatg gccaaacatt
    11641 ttggccgcca ctggcatcta aaggtgcgca atacaaaaca ggctctggat ttaattgagg
    11701 ccaacaagcc tgggtttaaa gcatggatga agcgcaatat caaaacctat gaccgttacc
    11761 acatccagat caccaataaa cagggccaca agtggtctgt tgacgaaagt gaatatcaga
    11821 tgatggggca gtctgacaac attgccaaaa tccgcattac acctgttccg cgaggaagcg
    11881 gtggatctgc ttttgggtgg tttcagacgg tagtaggggc cgctttgttg gttgtatcgg
    11941 cggtagtgat gcctgcttta gcacctctcg gtttgtcact gatgatgggc ggcatatcac
    12001 aaatcatatc gccgcaagcc actaacgaaa gtgtgagaca ggcagataac tcgaactctt
    12061 attatttcga cggccctcaa aatacagaaa accagggcaa cccagtacaa cttatctatg
    12121 gcgaggaaat tctggttggc tcacaggttg tgagttcttc aatcacgatt gaccagctaa
    12181 tgtaaacaag gattttttga acatggaaca gttcaagaag aaaaagctac cgctgtttat
    12241 tgccggtgcg ggtggtaaaa aaagcagcaa aagctccagc cgtacaccgg ttgaagccga
    12301 cgataccgta aattctcgtg caatggccgc tatcctcgat cttcttgggg aaggggtaat
    12361 tggcggctta gtaaatggcg caagatctat ttttatcgac gatctgccga ttgtaaatga
    12421 agacggttcc tccaacttca gcggaatcac atgggatttt cgcgacggtt cgcaagacca
    12481 aactccaatg tctggctttg atttcgttga aacgcctaaa tccgtcaata tccagttaaa
    12541 aaaaacacat tatgtaacgg tttcaatcga taacgatgaa gctgatcggg ttcgtgtcat
    12601 catgaagttt ccttctttgc gaagcataga taaaaaaact ggtgacacga acggtacaac
    12661 cgttgagtac aagttccaga tagcaaatgg cgactcaaca tttgtagatg tggtcgcgga
    12721 aggtgaaaaa agcgttggca ttaagctaac agcaaagaag accggcgttt attaccgtag
    12781 ctacgagctg aagctgccta agcctggacg cgcatacaag gttcgtgtag tccgtattac
    12841 cgatgataac agtagccagt atctctataa cgatacatgg gtggattcaa tcggggaaat
    12901 tgtcgataca ccgatgaact atccaaactc tgttttggtt ggattgaagg taaactctga
    12961 gcaattcggc agcaccatgc catctcgctc ttatctggtg cgtggcctaa aaattcgagt
    13021 accttcaaac tataacgagt ccagtaacac ttacgctggg gtatgggatg gcacttttaa
    13081 gctgttgtct tcttcgaacc ccgcgtggat tctctttgat ttgctgacca atgctcgata
    13141 tggccttggt cagtatgtgt cggaatctat gattgacctt ggccagttgt accagattgg
    13201 tcgatattgt gacgaggagg tcgatgatgg ctttggtggc aaagagaaac gctttgcaat
    13261 caatacgcag atcaccagtc gacaggacgc ataccgacta attcaggata ttgctggagc
    13321 attccgcggc atggtgtttt gggctggtgg gatggttaac atcatgcagg atagcccatc
    13381 agatccggta atgatgttta ccaactctaa cgtcaaagat ggattgttta cctataaagg
    13441 ttctgcgcgt aaagatcgcc catccgttgc gctcgtaacc tacaacaaca aggaggacgg
    13501 ttataagcaa aacatcgagt acgttgaaga tcaggacgca atgcgccgtt atggtgagcg
    13561 taagacagaa gtcgtagcat ttggctgtac aagccgaggg caggctcacc gagttggttt
    13621 atggctttta tataccgcca gaatggagtc ggatgtaatt acatttactg ccggcttaga
    13681 cgcgtcattt ctgatgcctg gtgaaaccgt tctgattcag aacaaatatc gcgcaggcaa
    13741 acgtaactca ggtcgaattg tggcgtttac caaaaatagc gtcacactcg atgcgcctgt
    13801 gtcgttagcc aaaggcggct gctttattcg aatactgaat caggaaggca aaatcgttga
    13861 acgcgatgtt cttgaaactg gcgaaaatat aacaaaagtt acgttttcaa aagccctgtc
    13921 ttcggctgaa acgcctgttt tgaacggcgt ctggactatt acagaaccag acctcgaacc
    13981 tatgcgcgtt cgcatcgtta acatcgcgca gggggaaacg ccgggtagct ttgacatcac
    14041 cgctgttgag aacaatccgt ctaaatatga ggcgatcgac aatggtgcaa cgcttatccc
    14101 gcaaaatacg acggtattgg acccgactta ctccaagccg tctaatttgc aaatcaccga
    14161 agggacttat ctctcaagcc ctggcaacct gtcagtaaaa ctgactgcaa catgggaagg
    14221 gaaatctcca gagtattgga tcagttggcg acgttctgat gaaaacaatg tatcgaactg
    14281 gcaatcggcg cgtgtaaccg aagagcaata cgaaatcgtt aatgttgcgg agaatggacg
    14341 ctacgacttc cagctgtatg cggtttcatt caacggtaaa aaaacagaga ttatcagtac
    14401 cgtttatcag gtgctgggca caatgacacc gccagatgcc ccaacgtcat taacggccgt
    14461 tggagactat cgtaatgtga tactgaattg ggttaatcca gattcggtag accttgatca
    14521 cattaatgtt tacgcgtccc agaccaataa tctggatacg gcgaaactga tcgcagagtc
    14581 tgcaagcacc acctttacac atgctggtct tggggatagc gaaacgtggt attactgggt
    14641 tcgagcatcg aacaagcgag gtatgttaag tcctccgaac tcaaacttgg gtacagaggc
    14701 gacaactcgc gatgtactgt cgttcctgac tggcaaaatt acatcttccg agcttggtca
    14761 ggctctgctt gaggacatca acagcaaagc ctctcaagaa gccgttgacg agttaaatga
    14821 gcatatcaac cagagcgtcg aatctttgga gggggcggta aacgacgtta aggaagatat
    14881 agctgaatta gataagcagt tcagcgataa cctcgcggat tttgaaataa aattcaacga
    14941 gcgtagcgat gctttggaga acgcacaaac cgagcttaaa ggtgaggttt cggcaacgat
    15001 cgacaaggtc aatgaagcgt ttgaaaaaat tgatgctact gatgctgcaa ttgttgaaat
    15061 cgaaaatacc gtatctgaac acgataaagc tctcgctaat acagtcgagg caataaaggc
    15121 tgcaagagat gaagcggcgg ctcttattgc taaggaaagc gaggctcgtg ttgaaggcga
    15181 tgctgcaaac gctaaacaac tggaagtgtt gcagtcaacg gtagaggaaa gctccgctgc
    15241 cgttgaagaa atgaaaaaga cggttgcaga ggtcgatcgt gcaagtgcag aactgactac
    15301 gaacattgag gcgttagcca aaacaaatat tgacctggct cttcgtcaag atgaagacca
    15361 gcacaagcag atggtcaata acgcgaagat cgcaacaaca cagaaaacct ttgctgatga
    15421 tatgtctgca atggctacga aggttgaaga aattcgcgta gaaattggcg aagacattaa
    15481 ggccagtatt ctggaagagt ccaccgcgcg tgccgatggc gatgaagcgt tagcaaagcg
    15541 tgtcactcag ctgcaatcta agtttgaagg agatatcagc gcggcgatta gtacagaaca
    15601 ggaggctcgt acatctgccg atgaagcttt gacgaaacaa ataactcagc ttgagtcgaa
    15661 agtaagcaac gatattgtcg cagccattaa ggaagagcaa gaagcccgag caacggaaga
    15721 ttcggctctg gcaagccaga tcacccaact tcaggcaaaa gttgatgatg atatttctgc
    15781 tgcaatacgc agtgagcagg aggctcgtgc gagcggagac tctgcgttag ctaaacaaat
    15841 aaaccagctt caagcaaagg ttgacggtga tatatccgcc gccatcactc aggagcagga
    15901 ggccagagca agtgctgacg aggcgctctc acgagaaatt aacagcctgc gagcacagac
    15961 cggaactgat attgccgcag cggttgctgt agagacaaag gcaaggactg atgccgatag
    16021 tgcattgtcc actcagatta cgtctcttac agcaaaagct aatgatctcg aggcatctct
    16081 tgccagagaa acaacggctc gtgcagatgg tgataccgct ttaacaaaag aggtttcaag
    16141 tttaaaagca cagactgcga aagatattag tgcggccgtt gcagttgaaa cgcaggctcg
    16201 aactgatgct gactcggcgt tgtcatctca gatcaccaag ctgacctctc aatacaaaga
    16261 ggatattaaa gcggctgtag caaccgaaac gaaaacgcgt acagagcaag atgctgcatt
    16321 ggcgactcaa atcacaaact tagagtctca aacggcagcc aacatttccg cggcggtaac
    16381 aactgaaacg acagcgagaa cgcaggcaga taatgctctg agtgggcgaa tcgatactct
    16441 gaaagcagag gtggatggaa ataccgcgat aattcaacag caggcaacag ctattgctga
    16501 taccaacaaa aaagtatcaa ccgcgtggac gttgaaaatg gagacttcaa cgagcggagg
    16561 gcagaagtat gttgccggta ttgcgcttgg tattgacacc actgggctat ctcagttttt
    16621 ggttcaggcg gacaggtttg gtttggtcaa ctctgttaac gggaagatca caacgccatt
    16681 tgttatcgaa aacagtatcg cctatatgaa tggagcgtat atcaaggatg gcacaatcac
    16741 caatgcaaaa gtaggcgatc tgcaatctac caattttgtt agcggtagat ctggatggcg
    16801 gttcggcaaa aatggaacgc ttgagatcaa cggtaatagc ggcggcaatg ggcgattggt
    16861 tataaatggt cagcggattg acgtttatga cgataacaac gtcctacgag taagaattgg
    16921 ccgtctgtga tggtggaaaa aaatttttat atcggtaaaa ataggtaagt atttacctaa
    16981 tggcgggcaa ggatagcccg ccaaatcaag gagcatattc aacatgtggt acagggaagg
    17041 tactatcaca tttacacagg gtagcaatac tctggttggg gcagggacag cctggaacgt
    17101 aacagctaat ggtgtgttgc cggggatgat cgtcattggc cccgacaata agctgtatga
    17161 gatcaagcgc gtaaccagtg atacgaacat tgttctctca gaaccttata ccggcgaaac
    17221 tcagtctgaa gttccgtgcc gaatcattac gacctatgaa ggcgacttaa cacagtttag
    17281 cgcgcgcttt actgcgctaa tgtcgcgtat gtcggctgat tccaagtcca tgcgcagttg
    17341 gttgactgcg ctggatgagg tgacaatcga gcgtgaagac ggtacagaag tgaccgttaa
    17401 gccgctaatg cagatcgtca acgagcacaa cgaaaacgtt gagtggtata aaaataacac
    17461 tgacgcgatt gatgctgctg gcgacaaagc tcgtgaggcg gcggccagtg ctgccgcagc
    17521 agcagaaagc gccaataccg ctggagaaaa agcctctcaa gcgtctcaaa gtgcatccgc
    17581 tgcggcatcc tcacaaagtg ccgcaagtgc gagtgcaact gctgcgaaaa aatctgaaac
    17641 gaatgccgca gcgtcacaac aatcagcagc cacttctgca tccaccgcga ccacgaaagc
    17701 gtcagaagct gccacctcag cccgggatgc ggcggcctca aaagaggcag cgaaatcatc
    17761 agaaacgaac gcatccttga gcgccagtag cgcagcttcc tcggcaacgg cggcaggaaa
    17821 ttccgcgaag gcggcaaaaa cgtccgagac gaacgccagg tcttctgaga cggcagcggg
    17881 acagagcgcc tcagctgcgg caggctcaaa aacagcggct gcgtcgtctg ccagtgccgc
    17941 gtcaacaagt gccgggcagg cctcagccag tgccaccgcc gccggaaaat cggcagaaag
    18001 cgccgcatca tccgcttcaa cagccacaac gaaggctggc gaagccactg aacaggccag
    18061 ctcagcagcg aggtctgctt ccgcagcgaa gacatccgaa acgaacgcga aagcgtcgga
    18121 aaccagcgca gaatcctcaa aaacggctgc cgcatcgtcc gccagttcgg cggcgtcatc
    18181 ggcatcatct gcgtctgctt caaaagatga ggcgaccaga caggcgtcag cagcgaaggg
    18241 cagtgccacg acggcatcca tgaaagcaac agaggcggca ggcagtgcga cggcagcatc
    18301 tcagagcaaa actgctgctg aatccgctgc gacccgtgcg gaagctgctg ctgatcgtgc
    18361 tgaagagatt gccggtgcag ttgcgatgga agacgcaagc cttacaacta aaggtgttgt
    18421 gaaacttagc agtgctgtcg acagcaccag tgaatcgctg gccgcaacgc caaaagcagt
    18481 taaagcagcc aatgacaatg cgaatagcag ggtgccatct aaccgaaaag ttaacggtaa
    18541 agcactgact gcggatatca cattaacgcc gaaagatatt ggtactttaa attcagtaac
    18601 gatgtctttc tctggcgggg ctgggtggtt caaactggct acggttacca tgccacaagc
    18661 gagttccatc gtttacatcg cattgattgg tggcgctggt tacaacgtcg gctccccaca
    18721 tcaggcaggc atttcagaac tggttctacg agcaggcaat ggaaacccca aagggattac
    18781 aggtgctttg tggaagcgta cagctgtcgg attaacgaat ttcgcctgga tcaacacatc
    18841 cggcgataca tatgatattt acgttgagat tggcaattat gcgacgagtg taaatatcca
    18901 ttgggattgt actgcaaatg cgacagtttc tatttataca tcgccaacat attcagcgag
    18961 taagccttcc agcgttaccg atggtgttgt ttatacgatg tatagcacac atcagaaacc
    19021 gacgccgtta gatattggtg cactgccaac aaccggagga acagtttcag gtccgttgtc
    19081 tgttactggt gggatcaccg gaacattaaa tggtaatgca agtacagcaa cgaaattgca
    19141 gacggcaaga tctatcggtg gagttggttt cgacggttct gcaaatatca accttccagg
    19201 tgtaaatact acgggtaatc agaacaccac tggtaatgct gcaactgcta caaaacttca
    19261 gacggcaaga actatcggcg gcgtgagctt tgatggtact gcgaatatta atttgccagg
    19321 tgttaatacg actggtaatc agaatacaac gggcaacgcg gctactgcta cgaagttgca
    19381 gactgcgcgt actattaatg gggtgtcgtt tgacggctcg gcaaatattt ccttgtcgcc
    19441 agcaaatata ggttgcccgg catctcctac tggttggtta actacaggaa gtaatggcgg
    19501 agcaataaca acagcacagt tagtgacgtt attgcaaaat aatggagcat ttaacacaaa
    19561 gtcatggatt gctcgatgtg cgtgggccta tgccaatagt gcaaccatac caaatagtga
    19621 aactggttgt ggcgttattc cattggcagg agctgttata gaggtattta ataacggtag
    19681 tagctcaaac aattatacga tccgtataac aacggccaca acgacgagtg tctctggtgc
    19741 tctcactaat gcggagttta tctatgtatt taatggcaca gattattctc caggatggcg
    19801 aagagtatat aacacgaaaa acaaaccaac agcctctgat gtcggtgcat tacctcttac
    19861 cggtggtaca ttatctggag gtttgacatc ttctggcgag atcatttcaa aatatgcaaa
    19921 tggtttccgc attgcttacg gtagctttgg gttctttatc cgtaatgatg gatcgaacac
    19981 atatttcatg ctaacagcat caggagacac attaggttca tggaacggtt tgcgacctat
    20041 tacaattaat aataccagcg gtgctgtatc aattggtaat ggactaaatg tgactggtgg
    20101 cgtaaatggt agtttgaacg gtaatgcttc aacagctacg aagttgcaaa cagcgagaaa
    20161 catcaatggt gttaagtttg atggctcagg cgatatcaac attaatacat tggtatctcg
    20221 tggccgagtt acggcgttaa gcggttctgc tcaaggtact gctggcattc aaatgtacga
    20281 ggcgtacagc aatagctacc cgaccacgta tggcaacgta ttgcacatga aaggtgcgag
    20341 tgctgctggt gagggcgagt tgcttattgg ctggagtggt acgagcggtg cacatgcgcc
    20401 ggttttcatt cgctcccgaa gagataccac tgatgcggca tggtcagcgt gggcgcaggt
    20461 atataccgct aaggattcaa tacctggtgt gaatacaacc ggtaatcaga acactactgg
    20521 taatgctgcc accgccacaa aattgcagac agcaaggaaa attgctggtg tggcgtttga
    20581 tggctctgcc gatattactt tgactgcggc taaccttaat gcctatacga aaacagaggt
    20641 aacaaacctt ctaagttcct atgtgaagag cagtgctctt ccaagtatga ctgtgcgaac
    20701 ttcctctgtt tcaggtgggg atatgggcat gagtttgtcc acattcatta gccacctaaa
    20761 atcaaatggg gcgtttagca aaagttattg gattggcttt ggcgatgcta tgggattcaa
    20821 tgctggcagt atcaacaata taacgggatt tggtgctgta gaattagcag aatctataat
    20881 cgaagttttt aatctaccta acggtgatta tacaattcgt ttaacaacgt cacataaagc
    20941 tgactacggt ggagttacaa acgcaatcct cgtttatcac taccgtagta atagaagtcc
    21001 gtcaggtcaa tggctgaaat ttgcaggtac tgttggggca acaagtaact gatttagaca
    21061 ttatttatat ggctgtgagg tttaaaatgc gatctcacag ccgcaatcat agggatatat
    21121 tagtgagtta ctagatacga atttgaacag ccgttaaacg gtatatgcat aggtaggtaa
    21181 gtatatacct ataattgaaa ggagaagtgt gatataaatc cgccatcccg atttgacttt
    21241 tcatggagga aaacatgtcg aacgagatgg cgggcgttac aacagagcaa gttgagcgta
    21301 ttgccgcgat cgttgctcgg gaggttgttg gcaaattagg taaagagcta cgtgaagaaa
    21361 ttggccagga ggtcaatgat cagctgaaaa cctactttgg tgatatgacc ccggcgcaac
    21421 atagtattca acactccaat ctggacaaac tccttaaccg gttagattcc atctcaagtg
    21481 ggttctttgg cggcattgtt tctaaaataa cgtcgttcat tattaccgca ctgcttttgg
    21541 ggttagccgc gtatggcgta aaaagtggac tgcaataaca ggagatcaag gatgaagact
    21601 ccgagaggca ttcgtaataa taaccccggt aatcttgata aaggatcacc gtggcaaggt
    21661 ctggttgcga atccagacga accgcgcttt tgcacgttta aagaccctgt ttgggggatt
    21721 cgtgcgctgg cggtgactct aattacctac cacgataaac gtcgcgcaaa agacggcaca
    21781 agtatcgata ccattcgtga agttattgaa cgctgggcac cgccgaatga aaacaatact
    21841 gacgcctaca ttaatgaggt gtctaaagcc gttggtgtaa ccgcagacat gatcatcgat
    21901 ctgcatgatt acgacatcct tcgacctttg gttgaggcaa tcattcgcca cgagaatggc
    21961 cgaggcccgc taaaaacgct gaacacctgg tatgcggcag aagttattga ggaaggtctg
    22021 cgtcgagctg gcgttgttaa gccggtgaaa accgtgaagg ctgttcctgt aactaaagaa
    22081 accgcaggcg caactgttac agcaggtatt ggtctggcgc agctggccga tgttatgccg
    22141 caggtttccg ctgctatgga taaagcacaa ggtcatatct ctagcgggga tacagtacgc
    22201 atcatcttcg gtattgccac tattgttgtg gcaggattca ttgcctggtc gcaggtaaga
    22261 aaacaccaga aagggatggt ctaatatgct aggcagcctg atgacaaagc taaaagttgc
    22321 tttgattacg ctggctgccg ttcttttcgt tcttgtcggc gcttacacga tgggcgggaa
    22381 tgcggcgcga cgagcaatgg aagagaaggc aaaacaggaa gacagaaaac gacttcaaaa
    22441 cacagtgaaa gtggtgaatg agacgagcag taaaatacgt cagaaagatg cttctgccgt
    22501 tcatcgtgag ttgtatgata agtgggtgcg tcattaaacc acaaacagcc agcgtgttgt
    22561 tctgcgatgg ggctgagcct atctatatca gcaataatga tgtaatgact gaagaaaccg
    22621 aacgccaaat cctttttcac aatacgatgg gagagagggt ttgtggttgg tgatgtcgaa
    22681 gttcccctca aatgagggga acacattatt cttcgaacaa tttttcgata gattttgtag
    22741 gatagaacaa agaacgctcg ttgttaccaa ccagctggat aaagcctaaa cttttataaa
    22801 acgccttggc tttgtcattt aacgcctcaa cgaaaagtcc atgaatgcct acagcaagag
    22861 atgcattgta cacaacgcgc attgcatgtg ttacaagcat tgagccaaat ccttgacctt
    22921 gaagggactt gtccaaagcc agcctaccca aagtaacact tggaacattc cgataaggaa
    22981 ccttcttttg tttgcttctt gaaggtaagg actccttttc aaaacaacta cctgacaaag
    23041 tgtaatatcc taacactttt ggcctttctt cttgagtgca aagcacataa gcacgaagaa
    23101 tttttccctc atgctgcctt tttaagtggt tggctaaaaa ggcgtttagt gactcttcgc
    23161 cgcaatcaaa accgtttaga tcataatctt tctctccaga gaaaatctct atcgttgtat
    23221 tgctcacgag taaactactc catgcttttc agacgatcag cagctcgttt cagcttgtcg
    23281 ttcggtgccg gaggattgct tatagcgtcc ataaccagat tccaggattc ttcattcaga
    23341 actagtctac ggtgttgctc tataactttc acggcacgtt cagatgcact gctaaccata
    23401 aactgagtaa tgctctggtt agacattgcg gcagcttcct cgatgatgct tttatcgtca
    23461 tcggttaatc tcaaatcgat gcgctgcttt tttagtgctg acatgtattc ctcctgatgg
    23521 ccgtgatgta ggaaggccat caatctcctc tgttcaaact gagttttcga ctcaaaaatt
    23581 actttaaaag gctatagccc attacgtgtg cattatcaaa ttgtgtacgg catatcaccg
    23641 tacttgtaat atagaggtca ttgttacttt tttcaacaga caaatacaag ctatgattgc
    23701 caattgttaa tcacaaccta ccatttaacc tttacaccgc agccgtaggc atttaggcta
    23761 tatcacatat aagaaaacaa gttgtttcag acgataatta tatacgcaaa gggaactctc
    23821 caatgaccaa gatctttgtg gttggcggca caaagggcgg gcctggcaaa tccaccgttg
    23881 cccagcaaat tgccgtttgc ctgaaagtca aaaagaagaa gaaggtttat attaccgata
    23941 tagatattca gcgcacgaca acgagctggt gtgaagaccg tcgacagaac gaagaccttg
    24001 agctgattcc ttttgcatac gttcaggatg acatcattaa gcacctaaaa tcgcttcagg
    24061 gtagagctga gtttgtagtg gtagatgctg gtggcttcga ctccgaaatt cagcgacaag
    24121 cgatgctgat ggctgacgtt atcattatcc cgctgcgtcc taagcgtcgt gatttgaaat
    24181 ctttacgtga catcgatcct attatcgaca atgttcgcaa tgtaaacgat aaagtgaagg
    24241 tccgcgcggt catgaaccag tgcccggctt tgccatcaca agtgtctcgc attctggcgg
    24301 ctaaagagat tgttgagacg tttggaatcg agtctgcgcc agtcaatcta tataaccgca
    24361 acgtctatga tgatgcggaa gagtctggtc gttctatctt tgaaatgacc ggtagcgagc
    24421 gcgacaaaaa ggcggaagcc gagtttgaag aatttgtaga ttatctgttg agtctggagg
    24481 aagaagaata atgtccatga aaatgggtga cctagcaaag cgcaaagagc ctgatgcacc
    24541 ggctaagaac acaactcctt tgcgccaacc agtcagacca cagggacgcc cgactcgtgg
    24601 caaagagaaa attaaaagcc gcacaatgtc actggaggac gaatacttcg aactgctgga
    24661 gatgatgaag ttcatccctc gcttcgagaa gttcactcgt tctgacgtga ttcgagcagc
    24721 cattttccat ctggcagaga agtcaccgca ggaaatcgag gacatcgtga agcttaacga
    24781 ggcgatcaca gctgccgacg ttacgatgcg taccgatgaa attaaacgag agttgatgaa
    24841 gaaaggttga ttatattggg cgtttgttac gcccaatata tga
//
LOCUS       DBANUU010000068        24786 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000068 DBANUU010000000
VERSION     DBANUU010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.068"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            116..547
                     /locus_tag="ACIE68_002658"
                     /pseudo
     CDS             116..547
                     /locus_tag="ACIE68_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
     gene            614..1594
                     /locus_tag="ACIE68_002659"
     CDS             614..1594
                     /locus_tag="ACIE68_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="HFX5908954.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
                     DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGKVKTLKQHPRKNKTAINIEYMKASIRAKVE
                     HPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSH"
     gene            complement(1703..2518)
                     /gene="ybcM"
                     /locus_tag="ACIE68_002660"
     CDS             complement(1703..2518)
                     /gene="ybcM"
                     /locus_tag="ACIE68_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFX5908955.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLTSRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKVRISGEILLG"
     gene            complement(2528..3079)
                     /gene="ybcL"
                     /locus_tag="ACIE68_002661"
     CDS             complement(2528..3079)
                     /gene="ybcL"
                     /locus_tag="ACIE68_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFX5908956.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(3248..3580)
                     /gene="emrE"
                     /locus_tag="ACIE68_002662"
     CDS             complement(3248..3580)
                     /gene="emrE"
                     /locus_tag="ACIE68_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFX5908957.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            3761..3880
                     /locus_tag="ACIE68_002663"
                     /pseudo
     CDS             3761..3880
                     /locus_tag="ACIE68_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(3914..4228)
                     /gene="fliE"
                     /locus_tag="ACIE68_002664"
     CDS             complement(3914..4228)
                     /gene="fliE"
                     /locus_tag="ACIE68_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFX5908958.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            4443..6101
                     /gene="fliF"
                     /locus_tag="ACIE68_002665"
     CDS             4443..6101
                     /gene="fliF"
                     /locus_tag="ACIE68_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFX5908959.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QFNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGKGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            6094..7089
                     /gene="fliG"
                     /locus_tag="ACIE68_002666"
     CDS             6094..7089
                     /gene="fliG"
                     /locus_tag="ACIE68_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFX5908960.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            7082..7768
                     /gene="fliH"
                     /locus_tag="ACIE68_002667"
     CDS             7082..7768
                     /gene="fliH"
                     /locus_tag="ACIE68_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFX5908961.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTLTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            7768..9141
                     /gene="fliI"
                     /locus_tag="ACIE68_002668"
     CDS             7768..9141
                     /gene="fliI"
                     /locus_tag="ACIE68_002668"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFX5908962.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            9160..9603
                     /gene="fliJ"
                     /locus_tag="ACIE68_002669"
     CDS             9160..9603
                     /gene="fliJ"
                     /locus_tag="ACIE68_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFX5908963.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNNDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKRMDEFAQRAAMRKPE"
     gene            9600..10727
                     /gene="fliK"
                     /locus_tag="ACIE68_002670"
     CDS             9600..10727
                     /gene="fliK"
                     /locus_tag="ACIE68_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFX5908964.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLLVPVSIQGRVTGNSGVDIFA"
     gene            10832..11296
                     /gene="fliL"
                     /locus_tag="ACIE68_002671"
     CDS             10832..11296
                     /gene="fliL"
                     /locus_tag="ACIE68_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFX5908965.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            11301..12305
                     /gene="fliM"
                     /locus_tag="ACIE68_002672"
     CDS             11301..12305
                     /gene="fliM"
                     /locus_tag="ACIE68_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFX5908966.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            12302..12715
                     /gene="fliN"
                     /locus_tag="ACIE68_002673"
     CDS             12302..12715
                     /gene="fliN"
                     /locus_tag="ACIE68_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFX5908967.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSISSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            12718..13083
                     /gene="fliO"
                     /locus_tag="ACIE68_002674"
     CDS             12718..13083
                     /gene="fliO"
                     /locus_tag="ACIE68_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFX5908968.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            13083..13820
                     /gene="fliP"
                     /locus_tag="ACIE68_002675"
     CDS             13083..13820
                     /gene="fliP"
                     /locus_tag="ACIE68_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFX5908969.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            13830..14099
                     /gene="fliQ"
                     /locus_tag="ACIE68_002676"
     CDS             13830..14099
                     /gene="fliQ"
                     /locus_tag="ACIE68_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFX5908970.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            14107..14892
                     /gene="fliR"
                     /locus_tag="ACIE68_002677"
     CDS             14107..14892
                     /gene="fliR"
                     /locus_tag="ACIE68_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFX5908971.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            15182..15805
                     /gene="rcsA"
                     /locus_tag="ACIE68_002678"
     CDS             15182..15805
                     /gene="rcsA"
                     /locus_tag="ACIE68_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFX5908972.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(15849..16037)
                     /gene="dsrB"
                     /locus_tag="ACIE68_002679"
     CDS             complement(15849..16037)
                     /gene="dsrB"
                     /locus_tag="ACIE68_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFX5908973.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            16200..16427
                     /gene="yodD"
                     /locus_tag="ACIE68_002680"
     CDS             16200..16427
                     /gene="yodD"
                     /locus_tag="ACIE68_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFX5908974.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            16725..17540
                     /gene="yedP"
                     /locus_tag="ACIE68_002681"
     CDS             16725..17540
                     /gene="yedP"
                     /locus_tag="ACIE68_002681"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFX5908975.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLTRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(17537..19231)
                     /gene="dgcQ"
                     /locus_tag="ACIE68_002682"
                     /pseudo
     CDS             complement(17537..19231)
                     /gene="dgcQ"
                     /locus_tag="ACIE68_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
     gene            complement(19402..19584)
                     /gene="yodC"
                     /locus_tag="ACIE68_002683"
     CDS             complement(19402..19584)
                     /gene="yodC"
                     /locus_tag="ACIE68_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFX5908976.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(19663..20580)
                     /gene="yedI"
                     /locus_tag="ACIE68_002684"
     CDS             complement(19663..20580)
                     /gene="yedI"
                     /locus_tag="ACIE68_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFX5908977.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVEKMRGQAH"
     gene            20753..21673
                     /gene="yedA"
                     /locus_tag="ACIE68_002685"
     CDS             20753..21673
                     /gene="yedA"
                     /locus_tag="ACIE68_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFX5908978.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAAVIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(21662..22132)
                     /gene="vsr"
                     /locus_tag="ACIE68_002686"
     CDS             complement(21662..22132)
                     /gene="vsr"
                     /locus_tag="ACIE68_002686"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFX5908979.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(22113..23531)
                     /gene="dcm"
                     /locus_tag="ACIE68_002687"
     CDS             complement(22113..23531)
                     /gene="dcm"
                     /locus_tag="ACIE68_002687"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFX5908980.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(23598..24293)
                     /gene="yedJ"
                     /locus_tag="ACIE68_002688"
     CDS             complement(23598..24293)
                     /gene="yedJ"
                     /locus_tag="ACIE68_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFX5908981.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(24333..24613)
                     /gene="drpB"
                     /locus_tag="ACIE68_002689"
                     /pseudo
     CDS             complement(24333..24613)
                     /gene="drpB"
                     /locus_tag="ACIE68_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
BASE COUNT         6148 a         6483 c         6263 g         5892 t
ORIGIN      
        1 gctttttaaa ttcagagtgt gaataaaatt actcggcgta atctctgcgg gatattcata
       61 aagtttttcc tttcctggcc gaaaatcttg catcggtcca caggaaagag aaaccatgtt
      121 aaaacgtatc aaaattgtga ccagcttact gctggttttg gccgtttttg gccttttaca
      181 actgacatca ggcggtctgt tctttaatgc cttaaagaat gacaaagaaa atttcactgt
      241 tttacaaacc attcgccagc agcaatccac gctgaatggc agctgggtcg cgttgttgca
      301 gacgcgtaac accctcaacc gcgcgggtat ccgctacatg atggatcaga ataatattgg
      361 tagcggttca accgttgctg agctgatgga gagtgccagt atttcgctga aacaggcgga
      421 aaaaaactgg gcggattacg aagcgttgcc gcgtgacccg cgtcagagca ccgccgcagc
      481 ggcagagatc aaacgtaatt acgatattta tcacaatgcg ctggcggagc tgatccaact
      541 gttagggaag gtgcgaacaa gttcctgata tgagatcatc atattcatcc ggagcgcatc
      601 ccagagggac atcatgagcc atcaactcac cttcgccgat agtgaattca gcactaagcg
      661 ccgtcagacc cgaaaagaga ttttcctctc ccgcatggag cagattctgc catggcagaa
      721 tatgaccgct gtcatcgagc cgttttatcc caaggcgggc aatggccgac ggccctatcc
      781 gctggagacc atgctgcgta ttcactgcat gcagcattgg tacaacctga gcgacggtgc
      841 catggaagat gccctgtacg aaatcgcctc catgcgcctg tttgcccgat tatccctgga
      901 tagcgccctg ccggatcgca ccaccatcat gaatttccgc cacctgctcg agcagcatca
      961 actggcccgt caattgttca agaccatcaa tcgctggctg gccgaagcag gcgtcatgat
     1021 gacccaaggc actttggtgg atgccaccat cattgaggca cccagctcta ccaagaacaa
     1081 agagcagcaa cgcgatccgg agatgcatca gaccaagaaa ggcaatcagt ggcactttgg
     1141 catgaaggcc cacattggtg tcgatgccaa gagtggcctg acccacagcc tagtcaccac
     1201 cgcggccaac gagcatgacc tcaatcagct gggtaatctg cttcatggag aggagcaatt
     1261 tgtctcagcc gatgccggct accaaggagc gccacagcgc gaggagctgg ccgaggtgga
     1321 tgtggactgg ctgatcgccg agcgtcccgg caaggtaaaa accttgaagc agcatccgcg
     1381 caagaacaaa acggccatca acatcgaata catgaaagcc agcatccgtg ccaaggtaga
     1441 gcacccgttt cgcatcatca agcggcagtt cggcttcgtg aaagccagat acaaggggct
     1501 gctgaaaaac gataaccaac tggcgatgtt attcaccctg gccaacctgt ttcgggtgga
     1561 ccaaatgata cgtcagtggg agagatctca ctaaaaactg gggataacgc cttaaatggc
     1621 gaagaaacgg tctaaatagg ctgattcaag gcatttacgg gagaaaaaat cggctcaaac
     1681 atgaagaaat gaaatgactg agtcagccga gaagaatttc cccgcttatt cgcaccttcc
     1741 ttagctctct tggtgttatc ttaaaacgat tatggaacct ttcagtaaaa cgagaaggac
     1801 acttgtaacc attttccctg gcaatctcgc tgatgggttt tactgtcgtt tgtatagcag
     1861 acaacgcatt atttaacctc acgtcgtcca atatactttg aaaacttact ccctcgcttg
     1921 ccagacggcg atgcaatgtg gaaacagaaa tgtagagata tctcgctacc ttgtttgctg
     1981 tccattttgt tccaggttcg gatagcatta ggttataaca acgacttgtt aatgattgtt
     2041 tactatctga taaaagtaaa caattaacat gatgtgctcc taatgaaagt agaacgccca
     2101 ttgctaagtg ttcctgaatt tgcgttgaga agcctcggga aatagatgtt tttagttgct
     2161 cccaacaata tattaactcg ggattctgag gtaagcagaa acctgtcgtg ttacgaattt
     2221 gatcggttat cgcataaaga ttttggaact tttcaattag atcaatggga taataaagca
     2281 tttctgcaag ataaagacct gcttctgggt aattcgcaat ataaaattca tatccacagg
     2341 gaaataatat tatctgatta ttatcaacag ttagactatg cgtctcccaa ttgataactt
     2401 ttttcccctg acggatacga cataaagctg gcataagagg cttaaccctg tgaatctcat
     2461 gatgtttatg catccgtatt tcttcaatct ttaggttagt cctatctttt gccagcatac
     2521 tcttatccta ctttacctca taaacgggta ttatctcagc ggttgcaatt ttattggcat
     2581 taagcatata accaactaac gctccgctgg aattagaatc cacaggaatc ttatcagttt
     2641 ttagagccca tattttaaac tggtaatgat ggggtttatc tcctttggga ggacatgctc
     2701 caccaaatcc cgcatagcca aaatcatttc ggccttggac agcaccagtc ggaagttttg
     2761 taccatcacg tcttcctgca tcagcgggca aatatgttac cgttgctgga atattagcaa
     2821 cagtccagtg ccaccatccg ctgcctgtag gcgcatctgg atcatacaca gttacggcaa
     2881 agcttttggt gccttctgga gcaccagacc aggttaatga gggcgatgta ttacccccct
     2941 cacacccaaa tccagaaaat acatgagacg ttgtaagttg ctctcctgtt tttatttcat
     3001 tactagtgac ctgaaatgct gcggcctgcg cagaaaatgt tatgaatgcc aatacacttg
     3061 aaacgataag ttttttcata aaaacctctt tgttgtgacc tatcgttatt ttatttgatg
     3121 tgcattgatc tcattatgca aaaaggcgca atgatcgcgc aaatgcaatc ataattataa
     3181 agttgccccg aacattagcc ataatatgca caaatcagat tcctgttatc tcaaggatga
     3241 ttatttttta atgcggtgcg cttcgtgaca acaagttaat gactaacaca ccggcacaaa
     3301 tcaacatcat gcctatgatg gctggcaagt ccagtcgttg gccaaaaaat ccccaggaaa
     3361 gcaaactaat cagaacgata ccgactcctg accagatagc ataagcaatc cctgtaggta
     3421 tataagccag tgtctgagct aataaccaaa atgatgcaca atagcaaata attgtgccaa
     3481 cagagggcca taaccgtgta aacccctctg aaaacttcat taaggttgta ccaatgacct
     3541 ctgcaagtat tgcgccacca agataaatat aagggttcat agcatattct ttcctgttca
     3601 tactggagag aattgtacta cagtttgaac acaactcacc gttttcatcc tggtgtatgt
     3661 gtatccaggg ggctctggcg tggcaagaga taaaggcagg aacaataatg atcagaagga
     3721 accccaggct aacaaaagag ttcactcttc ctatgagcaa agcgaagaca aaacatgtta
     3781 tcgcgtttac ggatcgtgga tggcagaaaa ctttcaggat caggtatcca ttttaaacca
     3841 gaaattgagt gagtttgccc catccatgcc ccaagcggtg agattggaag taattaacaa
     3901 caggttacaa aacctacacc tgcatggaca tcacttcctg atacgccgcc accagcttat
     3961 tacgcacctg aatccccatt tgcatagaaa ctgaggcttt ttgcatatcg gtcatcacat
     4021 cgtttaacgc cacgccgggt tcaccgaggg tgaatttttc tgcctgcgtg cgggcagctg
     4081 tttgtgtatc gctaatgcga tcgagcgcgg cgtgcagctg cccggcaaaa ctaatggtcg
     4141 gttgcggcag tgaatcctgc gcacgcgcac tcatcgccgt agcctgtaac tggctgataa
     4201 ccccttcaat cccctgtatc gctgacattt tcgtctcctg gataatttct ggtagcaaag
     4261 cctaccagta agtcaataag acaaaggcgc taaatagcaa caaaaaaacg ggtttattgg
     4321 cggatagaaa aaaacgaaag cacaaataat ggcagcgtca atttttcgag tttgctgacc
     4381 cgggagtgag tcttgttcca ctttgccaat aacgccgtcc ataatcagcc acgaggtgcg
     4441 cgatgaatgc gactgcagcc cagacaaaat ctcttgagtg gcttaatcgc ctgcgtgcga
     4501 atccgaaaat tccattgatt gttgccggtt ccgcggcagt ggcggtcatg gtcgcactga
     4561 tcctgtgggc gaaagccccc gactaccgca cattattcag caatctttcc gatcaggatg
     4621 gtggcgcaat tgtcagccaa ctgacgcaaa tgaatattcc ttaccgcttc agcgaagcca
     4681 gcggcgctat tgaagttccg gcagataaag ttcacgaact gcgtctgcgc ctggcacaac
     4741 aaggtttgcc caaaggcggc gcggtcggtt tcgaactact cgatcaggaa aagtttggta
     4801 tcagccagtt cagcgaacag gtgaattatc agcgggcgct ggaaggcgag ctttctcgta
     4861 ccatcgaaac tatcggcccg gtaaaagggg cgcgcgtgca tctggcaatg ccgaaaccgt
     4921 ctttattcgt ccgtgaacaa aaatcccctt ctgcatcggt gacagtaaat ctgttacccg
     4981 gccgcgcact cgatgaaggg caaattagcg ccattgtaca tctggtttcc agcgcggttg
     5041 ctggtctgcc gccggggaac gtcacgctgg tggatcaggg tggacatctg ttaacccagt
     5101 tcaacaccag cgggcgcgat cttaatgacg ctcagttgaa atatgccagc gatgtcgaag
     5161 gccgtattca gcggcgtatt gaagcgatcc tgtcgcctat tgttggtaaa ggtaatattc
     5221 acgcccaggt cacggcgcag ctggacttcg ccagtaaaga acaaacagaa gaacagtatc
     5281 gccctaacgg tgatgaatct caggcggcgc ttcgttcacg ccagcttaat gagagcgagc
     5341 aaagcggttc cggttatccg ggcggcgtac cgggggcgtt gtcgaatcaa ccggcacctg
     5401 cgaataacgc gccaatcagc acgcctccgg caaatcaaaa taaccgccag cagcaggcga
     5461 gcaccaccag caatagcggg ccgcgtagca cacagcggaa tgaaaccagt aactacgaag
     5521 tcgatcgcac cattcgtcat accaaaatga acgtgggcga tgtgcaacgt ctgtcagtcg
     5581 cggtagtggt gaattacaaa accttgccag acggtaaacc attgcctctc agcaacgaac
     5641 agatgaagca aattgaagat ctgacccgcg aggcgatggg cttttctgaa aaacgcggtg
     5701 actcgctcaa tgtcgttaac tcgccgttca atagcagtga cgaaagcggc ggagaactgc
     5761 cgttctggca acagcaagcg tttatcgatc agttgctggc tgccggtcgc tggttgctgg
     5821 tactgctggt ggcgtggctg ctgtggcgga aagcggtacg cccgcagtta acacgtcgcg
     5881 ccgaggcgat gaaagctgta cagcaacagg cgcaggcccg tgaggaagtg gaagatgcgg
     5941 tggaagtccg cctgagcaaa gacgaacaac tccaacaacg acgcgctaac caacgtctgg
     6001 gggcagaagt catgagccag cgtatccgtg aaatgtctga taacgatccg cgcgtggtgg
     6061 cgctggtcat tcgccagtgg ataaataacg atcatgagta acctgacagg caccgataaa
     6121 agcgtcatcc tgctgatgac cattggcgaa gaccgggcgg cagaggtgtt caagcacctc
     6181 tcccagcgcg aagtgcaaac cctgagcgct gcaatggcga acgtcacgca gatctccaac
     6241 aagcagctaa ccgatgtgct ggcggagttt gagcaagaag ctgaacagtt tgccgcactg
     6301 aatatcaacg ccaacgatta tctgcgttcg gtattggtca aagctctggg tgaggaacgt
     6361 gccgccagcc tgctggaaga tattctcgaa actcgcgata ccgccagcgg tattgaaacg
     6421 ctcaacttta tggagccgca gagtgccgcc gatctgattc gcgatgagca tccgcaaatt
     6481 atcgccacca ttctggtgca tctgaagcgc gcccaggccg ccgatattct ggcgttgttc
     6541 gatgaacgtc tgcgccacga cgtgatgttg cgtatcgcca cctttggcgg cgtgcagcca
     6601 gccgcgctgg cggaactgac cgaagtactg aatggcttgc tcgacggtca gaatctcaag
     6661 cgcagcaaaa tgggcggcgt gagaacggca gccgaaatta tcaacctgat gaaaactcag
     6721 caggaagaag ccgttattac cgccgtgcgc gaattcgacg gcgagctggc gcaaaaaatt
     6781 atcgacgaga tgttcctgtt cgaaaatctg gtggatgtcg acgatcgcag cattcagcgt
     6841 ctgttgcagg aagtggattc cgaatcgctg ttgatcgcgc tgaaaggggc cgagcagcca
     6901 ctgcgcgaga aattcctgcg caatatgtcg cagcgtgccg ccgatattct gcgcgacgat
     6961 ctcgccaacc gtggtccggt gcgtctgtcg caggtggaaa acgaacagaa agcgattctg
     7021 ctgattgtgc gccgccttgc cgaaactggc gagatggtaa ttggcagcgg cgaggatacc
     7081 tatgtctgat aatctgccgt ggaaaacctg gacgccggac gatctcgcgc caccacaggc
     7141 agagtttgtg cccatggtcg agccggaaga aaccatcatt gaagaggccg aacccagcct
     7201 tgagcagcaa ctggcgcaac tgcaaatgca ggcccatgag caaggttatc aggcggggat
     7261 tgccgaaggt cgccagcaag gtcatgagca gggctatcag gaaggactgg cccgggggct
     7321 ggagcaaggt ctggcagagg cgaagtctca acaagcgcca attcatgccc ggatgcagca
     7381 actggtcagc gaatttcaaa ctacccttga tgcgcttgat agtgtgattg cttcgcgcct
     7441 gatgcagatg gcgctggagg cggcacgtca ggtgatcggt cagacgctaa cagtggataa
     7501 ctcggcactg atcaaacaga tccaacagtt gttgcagcaa gaaccgttat tcagcggtaa
     7561 accacagctg cgcgtgcacc cggatgatct gcaacgtgtg gatgatatgc tcggcgcaac
     7621 cttaagtttg catggctggc gcttgcgggg cgatcccaca ctccatcctg gcggctgtaa
     7681 agtctccgcc gatgaaggcg atctcgacgc cagtgtcgcc actcgctggc aagaactctg
     7741 ccgtctggca gcaccaggag tggtgtaatg accacgcgcc tgactcgctg gctaaccacg
     7801 ctggataact ttgaagccaa aatggcgcag ttgcctgcgg tacgtcgcta tgggcgatta
     7861 acccgcgcta ccgggctggt gctggaagcc accggattac aattgccgct cggcgcaacc
     7921 tgtgtcattg aacgccagaa cggcagcgaa acgcacgaag tagaaagcga agtcgttggc
     7981 tttaacggtc aacggctgtt tttaatgccg ctggaggaag tggaaggtgt cctgcccggc
     8041 gcgcgggttt atgccaaaaa catttcggct gaaggactgc aaagcggcaa gcagttgccg
     8101 ctcggtccgg cgttattagg tcgcgttctg gacggcagcg gtaagccgct cgatggcctg
     8161 ccctcccccg atacgacgga aaccggtgcg ctgattaccc cgccatttaa cccgttgcaa
     8221 cgtacaccga ttgaacatgt gctggacacc ggcgtgcgcc caatcaatgc cctgcttacc
     8281 gttgggcgtg ggcagcgtat ggggctgttt gccgggtccg gcgttggtaa aagtgtgctg
     8341 ctggggatga tggcacgtta cacccgcgcc gatgtcattg tcgtgggttt gattggtgaa
     8401 cgtgggcgcg aagtaaaaga ttttattgag aatatcctcg gtgccgaagg gcgtgcacgc
     8461 tccgtggtga ttgccgctcc ggcggatgtc tctccgcttc tgcgaatgca aggtgccgcc
     8521 tatgccacgc gcattgccga agattttcgc gatcgtggtc agcatgtgtt gctgattatg
     8581 gactccctca cccgctacgc gatggcccag cgtgaaattg cgctggcgat tggcgaacca
     8641 cccgccacca aaggttatcc cccgtcggtg tttgccaaat taccggcact ggtcgagcgt
     8701 gccggaaatg gcattagcgg cggcggatcg attaccgcgt tttataccgt gctcaccgag
     8761 ggcgatgacc agcaggaccc cattgccgac tccgcgcggg caatcctcga cggtcacatt
     8821 gtgctgtctc gccgactggc ggaagccggg cactatccgg ctatcgatat tgaagcgtcg
     8881 atcagccgcg caatgacggc gttgatcagc gagcaacatt acgcgcgagt gcgcaccttc
     8941 aaacagctgt tgtcgagttt tcagcgtaac cgagatctgg ttagcgtcgg cgcgtatgcc
     9001 aaaggcagcg atccgatgct cgataaagcc atcgccctgt ggccgcagct ggagggctat
     9061 ttgcaacaag gcatttttga acgcgcggac tgggaagcgt ctctccaggg gctggagcgt
     9121 attttcccga cagtgtcata acccaggaga taacggcaga tggcagaaca tggtgcgctg
     9181 gcgaccctga aagatctggc agaaaaagag gtagaggatg ccgcgcgcct gctgggtgaa
     9241 atgcgtcgcg gatgtcagca ggcggaagaa cagctcaaaa tgctgatcga ttatcagaat
     9301 gaatatcgca ataacctcaa taacgatatg agtgccggga tgaccagcaa ccgctggatc
     9361 aactatcagc agtttatcca gacgttggaa aaagccatta ctcagcatcg ccagcaactt
     9421 aatcagtgga cgcagaaagt tgacattgcc ctgaacagct ggcgagaaaa gaaacaacgt
     9481 ttgcaggcct ggcagacact gcaggaacgg caatccacgg cggcactgct tgcagaaaac
     9541 cgcctcgatc agaaaaggat ggatgagttc gcccagcgcg ccgccatgag gaaacctgaa
     9601 tgattcgctt agcgcccttg attaccgccg acgttgacac caccacattg cctggcggca
     9661 aagccagcga tgctgcacaa gattttctcg cgttgttgag cgaagcatta gcaggcgaga
     9721 caactaccga caaagcggcc ccccagttgc tggtggcaac agataagccc acgacaaaag
     9781 gcgagccgct ggtcagcgag attcttgccg atgcgcaaca agcggattta ctgatccctg
     9841 tggatgaaac accgcctgtc atcaacgacg aacaatccac atcaacacca ttaaccaccg
     9901 ctcaaacgat gacgttggct gcggtggctg gcaacaatac ggcaaaagac gaaaaagcgg
     9961 atgatctgaa tgaagacgtc accgcaagcc tgagcgccct ttttgcgatg ttgccgggtt
    10021 ttgacaatac gcccaaagtg actgatgtgc cgtcaaccgt gttaccggca gagaaaccaa
    10081 cgctcttcac aaaactgact tctgcgcaac tcacaacagc acagcctgat gatgcccccg
    10141 gcacgccagc tcagccatta acaccgctgg tagcagaagc ccagagtaaa gcggaagtca
    10201 tcagcacacc ttcaccggtg accgctgccg ccagcccgct aatcactcca caccagacac
    10261 agccactgcc caccgtcgcc gcgcctgttt tgagtgcacc gctgggttct cacgaatggc
    10321 aacaatcatt aagccagcat atttcgctgt tcacccgcca ggggcaacaa agtgcagagt
    10381 tgcgtctgca cccgcaggat ttaggtgaag tgcaaatctc cctcaaagtg gatgataacc
    10441 aggcacaaat ccagatggtt tcaccgcatc agcatgtacg cgccgcgctg gaagcagcgc
    10501 ttccggtact gcgtacgcag ctggccgaaa gtggcattca gttagggcaa agcaacatca
    10561 gtggcgaaag ctttagtggt cagcagcagg ccgcttccca acaacagcaa agccaacgca
    10621 cagtaaacca tgaacctctg gcgggggaag acgacgatac gcttctggtt cccgtctcta
    10681 tacaagggcg cgtaacaggc aacagcggcg ttgatatttt cgcctaacgt cagaggtagc
    10741 accgtaatcc gcgtcttttc cccgctttgt tgcgctcaag acgcaggata attagccgat
    10801 aagcagtagc gacacaggaa gaccgcaaca catgactgat tacgcgataa gcaagaaaag
    10861 caagcgatcg ctttggatcc cgattctggt attcattacc ctcgcggcct gtgccagcgc
    10921 aggttacagc tactggcatt cgcatcaggt tgccgctgac gacaaagcgc agcaacgcgt
    10981 cgtaccctcc ccggtcttct acgcgctgga taccttcacg gtcaatttgg gcgatgcgga
    11041 tcgcgtgctt tatatcggca taaccctgcg cctgaaagat gaagctaccc gctcgcggct
    11101 gagtgagtat ttgccggaag tccgtagccg tttgctgtta ctgttttcgc gtcaggatgc
    11161 tgccgtactg gcgacagaag aaggcaagaa aaacctgatt gccgagatta aaaccacgct
    11221 ttccacgccg cttgttgccg ggcaaccgaa acaggatgtc accgacgtgc tgtataccgc
    11281 ttttattctg cgataacgac atgggcgata gtattctttc tcaagctgaa attgatgcgc
    11341 tgttgaatgg tgacagcgaa gtcaaagacg aaccgacagc cagtgttagc ggcgaaagtg
    11401 acattcgtcc gtacgatccg aatacccaac gacgggttgt gcgcgaacgt ttgcaggcgc
    11461 tggaaatcat taatgagcgc tttgcccgcc attttcgtat ggggctgttc aacctgctgc
    11521 gtcgtagccc ggatataacc gtcggggcca tccgcattca gccgtaccat gaatttgccc
    11581 gcaacctgcc ggtgccgacc aacctgaacc ttatccatct gaaaccgctg cgcggcacag
    11641 ggctggtggt gttttcaccg agtctggtgt ttatcgccgt ggataacctg tttggcggcg
    11701 atggacgctt cccgaccaaa gtggaaggtc gcgagtttac ccataccgaa cagcgcgtca
    11761 tcaaccgcat gttaaagctg gcgcttgaag gctatagcga cgcctggaag gcgattaatc
    11821 cgctggaagt tgagtacgtg cgttcggaaa tgcaggtgaa atttaccaat atcaccacct
    11881 cgccgaacga cattgtggtt aacacgccgt tccatgtgga gattggcaac ctgaccggcg
    11941 aatttaatat ctgcctgcct ttcagcatga tcgagccgct acgggaattg ttggttaacc
    12001 cgccgctgga aaactcgcgt aatgaagatc agaactggcg cgataacctg gtgcgccagg
    12061 tgcagcattc acagctggag ctggtcgcca actttgccga tatctcgcta cgcctgtcgc
    12121 agattttaaa actgaaaccc ggtgacgtcc tgccgataga aaaacccgac cgcatcatcg
    12181 cccatgttga cggcgtcccg gtgctgacca gtcagtacgg cactctcaac ggtcagtatg
    12241 cgttacggat agaacatttg attaacccga ttttaaattc tctgaacgag gaacagccca
    12301 aatgagtgac atgaataatc cggccgatga caacaacggc gcaatggacg atctgtgggc
    12361 tgaagcgttg agcgaacaaa aatcaatcag tagcaaaagc gctgccgacg cggtgttcca
    12421 gcaatttggc ggtggtgatg tcagcggaac gttgcaggat atcgacctga ttatggatat
    12481 tccggtcaag ctgaccgtcg agctgggccg cacacggatg accatcaaag agctgttgcg
    12541 tctgacgcaa gggtcagtcg tggcgctgga cggtctggcg ggcgaaccgc tggatattct
    12601 gatcaacggt tatttaatcg cccagggcga agtggtggtc gttgccgata aatatggcgt
    12661 gcggatcacc gatatcatta ctccgtctga gcgaatgcgc cgcctgagcc gttagtgatg
    12721 aataaccacg ctactgtgca atcttccgcg ccggtttctg ctgcgccact gctgcaggtg
    12781 agcggcgcac tcatcgccat tattgccctg atcctcgctg ctgcctggct ggtaaaacgg
    12841 ttgggatttg cccctaaacg cactggcgtt aacggtctga aaattagcgc cagtgcttca
    12901 ctgggcgcgc gtgaaagggt tgtggtggtc gatgtggaag atgcacggct ggtgctcggc
    12961 gttaccgcag gtcaaatcaa tctgctgcat aaacttcccc cttctgcacc aacggaagag
    13021 ataccgcaga ccgattttca gtcggtcatg aaaaatttgc ttaagcgtag cgggagatcc
    13081 tgatgcgtcg tttattgtct gtcgcacctg tccttctctg gctgattacg cccctcgcct
    13141 tcgcgcaact gccgggtatc accagccagc cgctgcctgg cggtggacaa agctggtcgc
    13201 tcccggtgca gacgctggtg ttcatcacct cgttgacgtt tattccggca attttactga
    13261 tgatgaccag tttcacccgc atcatcattg tttttggttt attgcgtaac gcgctgggaa
    13321 caccctccgc gccacctaac caggtattgc tggggctggc actgtttttg acctttttta
    13381 ttatgtcacc ggtgatcgac aaaatttatg tagatgcgta ccagccattc agcgaagaga
    13441 aaatatcaat gcaggaggcg ctggaaaaag gggcgcagcc gctgcgtgag tttatgctgc
    13501 gtcagacccg tgaggcagat ttagggttgt ttgccagact ggcgaatacc ggcccgttgc
    13561 agggacctga agccgtgccg atgcgcattt tgctcccggc ctacgtgacc agcgagttga
    13621 aaaccgcatt tcagataggc ttcacgattt tcatcccttt tttgattatc gacctggtga
    13681 tagccagcgt gttgatggca ttggggatga tgatggttcc ccccgccacc attgctctgc
    13741 cctttaaact gatgctgttt gtactggtgg atggctggca attgctggtc ggttcgctgg
    13801 cgcagagctt ttacagttag agaggcaaaa tgacacctga atcggtcatg atgatgggga
    13861 ctgaagcgat gaaagtcgcg ctggcactgg ctgccccgct attgttggta gcgttggtca
    13921 cgggccttat catcagtatt ttgcaggccg ccacccagat taacgaaatg acgctgtcgt
    13981 ttattccgaa aatcatcgcc gtatttatcg ccattattat tgccggaccg tggatgctca
    14041 atctgttgct ggattacgtc cgcaccttgt tcactaacct gccgtatatc atcgggtagc
    14101 cgtactatgt tgcaggtgac aagcgaacaa tggctatcct ggttaagcct gtacttctgg
    14161 ccgttactgc gcgtgctggc gctgatctcc accgcgccga ttctgagcga acgcagcgta
    14221 ccgaaacggg taaaactggg tctggcaatg atgatcacgt tcgccattgc cccatcatta
    14281 cctgccaacg atgttcctgt tttttcgttc tttgctctgt ggctggccgt gcagcagatc
    14341 ctgatcggca ttgcgcttgg ttttaccatg caatttgcct ttgccgctgt gcgaaccgct
    14401 ggcgaaatta tcggtctgca aatggggctg tcatttgcga catttgtcga tccggccagc
    14461 catcttaata tgcccgtttt agcgcgtatc atggatatgc tggcgttact gctgttcctg
    14521 acatttaacg gtcatttatg gttgatttca ctgctggtcg atacctttca caccctgccg
    14581 attggtggcg aaccgttgaa cagcaatgcg tttctggcac tcaccaaagc agggagtttg
    14641 attttcctta acgggctgat gctggcgtta ccgctcatta ctctgctgct gacactgaat
    14701 ctggcattag gtttacttaa tcgtatggcc ccgcaattat ccatttttgt tattggattt
    14761 ccattaactc tgactgtcgg catctcttta atggcggcat taatgccgtt aattgcgcct
    14821 ttttgcgaac atttattcag tgaaattttt aatttgctgg ctgatattat tagtgaactg
    14881 ccattaatat aattccgtaa cgtttatcat gttatcctaa ggattatccg aaaaataata
    14941 cctacgaaca tcttccagga tactcctgcg gcgaaatatt tgttttaagc tcactcacat
    15001 atcgcaacat ttactttact ttaagacaat tccaggcaaa ttatacaaca ctttacggga
    15061 tagtaagtcc gcctgaaaaa tcgcgagagt ggcgcattag gtgacccatg ttgttccgtt
    15121 tagtcatgat gaaatattca ggtaagggga attatcgtta cgcattgagt gagggtatgc
    15181 catgtcaacg attattatgg atttatgtag ttacacccga ctaggtttaa ccgggtatct
    15241 gttgagtaga ggggttaaaa aaagagaaat caacgacatt gaaaccgttg atgaccttgc
    15301 catagcttgt gattcacagc gcccttcagt ggtgtttatt aatgaggact gtttcatcca
    15361 tgatgcttct aacagtcagc gcatcaagca catcattaat caacatccca atacgttatt
    15421 tatcgttttt atggcaattg ccaatgttca ttttgatgaa tatctattgg tcagaaaaaa
    15481 tttattgata agttctaaat cgattaaacc ggaatctctc gacgatatcc ttggcgatat
    15541 tctgaaaaaa gagacaacga taacctcgtt tttaaatatg ccgacgttat cattgagccg
    15601 aaccgaatcg agtatgttgc gaatgtggat ggcaggtcag ggaaccattc aaatctctga
    15661 ccaaatgaat atcaaagcca agaccgtttc atcgcataaa ggtaatatta aacgtaagat
    15721 caaaacgcat aataaacagg ttatctacca tgtcgtccga ctgacggata atgtgactaa
    15781 tggtattttt gtcaacatgc gctaacacat tctgactggt ggtttcccac cagtcaggct
    15841 gaataagatt actctgcttt ctccacaaag ataccgtcct gatgccctgc ttcattaaag
    15901 aaccagatgc cgagcgggta gtcttccagc gaaaccaggt acattgtgcc ttcactaaac
    15961 tcctcaactg ccagtaccac gccaggacga cgcggaccgc catccgtttt gactgttacc
    16021 cgatcattca ccttcatcgt tttcctcctg tggctttgtg ccagtgtaga acaatttcgt
    16081 ttttctggca gcgccaggcg cgcgcgagtg ctgattttct cgacggtcta tacttaagag
    16141 aagccaggcg gacttaacga ctggcggcaa caacagagta acggttgcga ggaaagatga
    16201 tgaaaaccgc aaaagagtac agcgataccg caaaacgtga ggtcagcgtc gatgtcgatg
    16261 ccctgctggc ggcgatcaat gaaattagcg aaagcgaagt tcatcgcagc cagaacgatt
    16321 ctgaacacgt tagcgtcgat ggacgtgaat atcatacatg gcgtgaattg gcggatgcct
    16381 tcgaactgga tattcatgac ttcagcgtct ctgaagtgaa tcgttgaatg cacaataaaa
    16441 aaatcccgac cctgaggggg tcgggatgaa acttgcttaa gcaagaagca cttaaaaaat
    16501 tcgttacacc aggaaatctg atgtgttcat caccttatcc gcaatttttt tcgctgacaa
    16561 gaaaatattc gccatatgaa tgattaacgc tacttatgaa tagaaatgtg acgcacgtca
    16621 ccccttggtg gctctggcgt cagcgggatc gctcccgaaa aaattatctt tgataatttg
    16681 tgctgttttg agaatcatcc tggtaagggg agtactgcgc gactatgttt tcaattcaac
    16741 aaccactact ggtttttagc gatcttgatg gcaccctgct ggacagtcat agttatgact
    16801 ggcaaccggc agccccctgg ctcacccgtt tacgcgaagc aaatgttccc gtcattctct
    16861 gtagcagtaa aacatcagcg gaaatgctgt acttgcaaaa aatgttgggg ctacaaggtt
    16921 taccgctgat tgcagagaat ggcgcagtga tccagcttgc tgaacaatgg caggagatag
    16981 acggttttcc acgcatcatc tcaggtatta gccatggcga aatcagccag gttttaaata
    17041 cgctacgcga gaaagaacat tttaaattca cgacttttga tgatgtcgac gatgcaacca
    17101 tcgccgaatg gacgggatta agccgtagcc aggcggcgct gacgcagctt catgaggcgt
    17161 cggtaacgct aatctggcgc gacagtgacg agcgtatggc acaatttacc gctcgtctga
    17221 acgaactggg cttacagttt atgcaaggtg cgcgcttctg gcacgtcctg gatgcctctg
    17281 ccggaaaaga tcaggctgcc aactggatta tcgcgaccta tcaacaattg tcaggcaaac
    17341 gcccaaccac acttggcctg ggcgatgggc caaacgatgc gcccttactg gaggtaatgg
    17401 attacgcggt gattgtgaaa gggctaaacc gtgaaggggt gcatctgcat gatgaggatc
    17461 cggcccgcgt ctggcgaacg cagcgtgaag gaccggaagg atggcgtgaa gggctggacc
    17521 attttttctc cgcccgttaa gcgttatcgc tcgcgaatac ccgattgcgc ccagcctgtt
    17581 tagcgagata aagccgacgg tcggccagtg actggagttg ttcaaaatca taatcaccgg
    17641 tttcctcgct gctacttacc cccaacgagg cactgatgcg tatcgtcgta ctcttagcaa
    17701 tcaacatctc tttttcattt aacttcaggc gaatacgttc tgcgacttcc gcagcctccg
    17761 tcagactcgc gcctggcaga atcacacaaa actcctcacc accgacccgc ccggcaacgt
    17821 cctgcgcacg caaggaactg ctaattaatc cggcagcatg agaaagaaca cggtcgcccg
    17881 cctgatgacc aaagcggtca ttaatcgctt taaaatggtc aagatcgacc tgaatgacag
    17941 aaaaaggatg ttggtgcgtc tgacacaatt tcgcgagcgg acgggctttt tcgaacagtg
    18001 cgccacggtt atataaacgc gttaaggtgt cgtgccacgc ctgccactgc aacgagcttt
    18061 gcagaacata catgttgctg accatccggc gaatcacata ccaggagatg agttacatgg
    18121 tggtaaagag cgcccacagc agggttaatg caatgctgat actgccgaaa tcgccgcgca
    18181 cgccttcgct taacgtatgg acgcgcacca gcacaccgtc gaaatggtcc agacgttccc
    18241 agctaacata gcgactgtcc atacgaatgc cgccccgcgt gtcatgttcc attgcttgcg
    18301 ccagcaaggc cagttcacga ggatcaaaaa tattccctgt tggatggtca ggattggaag
    18361 aggtcaaaaa tctcagctta ctgtcataga gctgatactc accatcgagg tttttatcga
    18421 tggcgtttct taaaaattgc tgcatagtac gcacgggaat actcatcccc agcacgccat
    18481 accagtagtt attactgtct accggaacac tgacggtaac ctgcggttca gtattgctgg
    18541 cgtgttccgg ttgcgaagtg aaccagcgta ccgcgcggtg acgattttct cgttgtgaat
    18601 ggccgataaa ccagggttgg gtgacatagc cgtaataacg cgtcgggaca ttgcgcgtaa
    18661 acaaggtcgg ctgcgtcgaa acgtaaaatc cggcacgcga gacatacatc gcctgttcta
    18721 ccatcgacga ggagttgtgt gccagtcgca gcaagtaacc aacttccagt gcagcggtaa
    18781 tttcattgtc gaggctttca ttttcgcgag acaggagatt cccctcactg actaatgcat
    18841 ccgacacgcc attgactggc agggtgcgcc gtcggttgag ttcgatttgc caggcgtgct
    18901 cgtcgcgatg ctgttcgaac tcggttacag catcacgcag agaggtgaaa tccagtggcg
    18961 ctacgagagc ttcgcgcatg ccattacgca aaaagatcag tttgtcgacg ttatactgca
    19021 aatgcttatc gagcgcgttg gctacgtttt ccagatgatt acgctggctg gagatatagg
    19081 catcttccag cactaccact tcacgccagg tcagcaaggt ggaaaaaagc aataccacga
    19141 taaagcagag attaacgata tgaccaggcc ccaggcggcg tgcgagtttt ttcaaccagc
    19201 tctggttttc catttttgtc tcgtgctgca ccctgacgac tccccacctg cttttttatg
    19261 attctggcat aacctggcga tagcgtaccg caaaacagcg ataattttat aatggtggca
    19321 tgataagacg cgactatcat tcattgcagg cattggcaca ccaggccgga tgtgatccgg
    19381 cctggtctta atgataccct ttcaaacttc ttccgcagaa cgactcccct cccccggaac
    19441 aagctcggtt tcatgaaaag cttcccgctt gaccccgtaa ccgtcatacc aacgacactc
    19501 aaccataccg ctggagtatc cagtgacaat catccgcggg ccgccctctt taaccgtaac
    19561 ttcctcacta accataaagc tcatactcgc ctcctttttt cgagtgaaac ttgttcacct
    19621 tagttgaaga tggcgaattt tgcataatag cctgtgcgag ttttaatgtg cctgaccgcg
    19681 cattttctct acggctttca ctcccagcac cacgatgcca ccgatgataa atccaagaat
    19741 cagatttaaa acagtcggta atatcatcgc caccactgca ctttgctgcc cggcgaaatg
    19801 ttcaatggcg tgatgcagcg gcgcaatacc atgtaccaca atcccgccgc cgacgaggaa
    19861 catcgccagc gtgccgacaa tcgataacgc tttcatcagc cagggcgcga taatcaataa
    19921 tcctttacct aatgcctgca tcagcgcgct ggatttttcc gccagccaat accccaggtc
    19981 atcaatctta acgataaccc ctaccagacc gtagacgccc acggtcacta ccagcgcgat
    20041 gcctgaaagc accagcacct gattaagcaa cggcgcttcg gccacaatcc ccagcgtgat
    20101 ggcgacgatt tccgcagaca atataaaatc ggtacgaatc gcccctttta ttttgtcctt
    20161 ttcaaacttc agcggatcct gtgccgccag cttctccaga cgctgctggc tctgcgccgg
    20221 atcttcctta tgtttacgcg cctccagcat atgcagcact ttctctaccc cttcaaagca
    20281 gagaaacgcg ccaccgatca tcaacaatgg cgtaatcgcc cacgggataa acgcactgat
    20341 gatcagcgcc agtggcacca gaatcacttt attgatcagc gatcctttcg ccacgcccca
    20401 gaccacggga agttcccggt tggcccgcac gcctgaaact tgttgcgcat tgagcgataa
    20461 gtcatcccct aatacaccgg cggttttctt cgccgccagt ttgcccatca cggagatatc
    20521 gtccagcagt gtggcgatat catcgagcaa cgtcagtaaa ctactcccgg ccaaaagcat
    20581 tccctctcat ttttattgtt gaagggtgta agtatgaagc aaaaacggca gctctgatac
    20641 ttgcttcaca ggtcaacatt tttttaacaa tcaagtgcaa ttatatctct cgccagcaga
    20701 atgattttca cgtttactat gaaccacctt tttatttcca ccgtgatgga ttatgcgttt
    20761 ccgccagttg ttaccgcttt ttggcgcgct gtttgcgttg tatatcattt ggggctcaac
    20821 ctattttgtc attcggattg gcgtggaaag ctggcctccg ttaatgatgg cgggcgttcg
    20881 attcctggca gccggtattt tattgctggc atttttgcta ctgcgcggac acaaactccc
    20941 cccgctacgt ccgctgctca atgccgcggt gattggcctg ttattgctgg ctgtcggtaa
    21001 tggcatggtg acggttgccg agcatcaaaa tgttccttcc ggcatcgccg ccgttgtggt
    21061 tgcaaccgtg cccctcttta ccctgtgctt cagccgcctg tttggcatta aaacgcgcaa
    21121 actggaatgg gtgggtatcg ccattgggct tgccggaatc atcatgctca atagcggtgg
    21181 aaatttaagc ggcaatccgt ggggcgcgat tctgatttta atcggctcga ttagctgggc
    21241 gtttggctcg gtttatggct cgcgcattac cttacctgta gggatgatgg cgggtgcgat
    21301 tgagatgctg gcggcaggcg tggtgttaat gatcgcgtcg atgattgcag gtgaaaaact
    21361 gacggcactc ccttcccttt caggcttcct tgcggtcggc tatctggcgc tgtttggttc
    21421 gattatcgcc attaacgctt atatgtattt aatccgcaac gtcagtccgg ctctcgccac
    21481 cagctacgcc tacgttaacc cggtggtcgc ggtcttgctg ggtacgggac tgggcggaga
    21541 aacgctgtcg aagattgaat ggctggcgct cggcgtaatt gtcttcgcgg tggtactggt
    21601 cacgttggga aaatatctct tcccggcaaa acccgtagtt gcgccagtga ttcaggacgc
    21661 atcaagcgag taaatgaatc ccctgcgtgt cgatctgcgc gctggcacct tcgccgcaga
    21721 tccactcttc cagacgctcg ctaagcgctt catccgtcag cttctcgcgc ccacgtaacg
    21781 cgcactccca gacaatcaat acgcgccagc cgagttcctg caagcgactg atatcgcggc
    21841 gatcgcgctc aacattttta cctatcttct ccagccagaa ctcggttcga gtcgcaggca
    21901 ctttaaacag atagcagtga tgatggtgcc agaagcagcc gtgggtaaat atcacgcaac
    21961 gatattcatc aacgacaaaa tccgggcgac cgggcagact ggcgtcctga acgcgaaacg
    22021 ccaggccctg cccggttaac agactggcga ggcgcttctc aatcgccgta tcacgcgtgg
    22081 caatcgcgcg catatttttg ctgcgagtgg ccttatcgtg aacgtcggcc atgttgtgcc
    22141 tcttgctgac gcaacgccac cgcctgtttg atttttggct caagcagttt tgccaccgcg
    22201 gcaaagaccg gcacgaccac cgagttaccg aactggcgat aggcctgagt gtccgaaacc
    22261 ggaatgcgga atttcgcttc tcccggcgct tcaaaaccca ttaagcgcgc gcattcccga
    22321 ggcgttaacc gacgtggacg atgttgctga ttcagcggat cgtcaaaatc tttctcaccc
    22381 gtggccatat cccagccgcg atcgattaaa atttccgcgc catctttgta ataacgcgca
    22441 gacagcgtac gcgtgacgct ttgcggattg ttcggataaa ccattccata accgaagccg
    22501 ttaccgcgcg cctgatgttt tttcgcatat cgatagaggt acttccacag caccggcgtc
    22561 aggatatatt tcgcctcgac catcgggtcc aacagctgcg ccagcgtcac tcgctgtgca
    22621 gggaaacatt cgctgatatc acgcagagta aaatcggctt tcagattaag atcgcgacga
    22681 aaacccacca gcacgatgcg ttcacggtgc tgcggcagaa aatgtttacc atcgatgatt
    22741 ttcggatcgt ccggcccgtt atcttctgca tcagccacgt catagcccag ttcgtccagc
    22801 gtctgcatga tgatgcggaa cgttttaccc tggtcgtgac ttttcaggtt tttgacgttt
    22861 tcgagcacaa acatcgccgg acgacgcgcg tcgataatgc gtaccacatc aaaaaacagc
    22921 gtgccctggg tatcgcaggc aaaaccgtgc gcccgcccga gcgagttctt tttcgatacg
    22981 ccagccagcg aaaatggctg acaagggaaa ccggccagta aaacatcgtg ttcaggaatg
    23041 tgttgacgaa tatgttccgc cgccgcctca tcactcacgc cttctttatg gctgagggtg
    23101 atgtcgcgga tatcttcatt aaaatgatgc gtcgccggat cgcaataatg gttggcttta
    23161 taagtgcgta ccgcatgttt gttccattcg ctggtaaaca cgcattgtcc gccaatcgat
    23221 tcaaaaccgc gacggatgcc gccaattccg gcaaatagat cgataaagcg aaacgcataa
    23281 tgcggatgat gtgccggtgg tttgggtaac aacgtttgca gatgggcgaa ctctttctca
    23341 cttaaacggt gccatgccga gtcattcgcc agcgcacgtt ttaaaattgc cgcgctccag
    23401 tgattctcac ccacaccatt aagctgcgcc accagcgttt taacatcata aatttgcagc
    23461 agtttctcca gcattgcctg tgcggcattc ccggtgctgt atgaatcggt tactgatata
    23521 ttttcctgca tagatttaac cggccatctg agatggccga caggttaaca taattacagc
    23581 catttagggc cacgcgctta tcccgcgggt gaaaacgcat ctatcacctt gtgatcgaca
    23641 ccttcattct cccccgccag ttcggcactg agcttcgcca taaactcgac taaaaaatgc
    23701 gcgttgtgct gcgccagctg cttgcctctt gcggtttgca tggtttgcgg tagcttcagc
    23761 aacttagtct ggaaatgatc cagcgcgtag cgtttatcat caagcgggcg atgctgtgcg
    23821 aacggatctt caccatcaaa cagcgccacg cccaatgccc ctgaaacggc aaacacgcgc
    23881 gccaggccaa tcgcccccag ggcttccagc cgatccgcat cctggacgat tttagcttcc
    23941 gttgttaagg gggcgatttg cgcgctgaaa ctgtgagcgg caatggcatg acaaacggcc
    24001 tcgattttct ccgccggaaa ttgctcgaac tcttcacgga gcaggcgacg cgtctcttct
    24061 gccgccagga ttgaagaacg ctgcctttgc ggatgatttt tcgccaggct cacaatatcg
    24121 tgaaaataac aggcggttaa aatcaccagc atatcgacat cgtcatctgc cgccagcttc
    24181 tgtgccgtag cccagacgcg gcgaaaatga cacacgtcat gggccgcgtc ctggtgctga
    24241 tgatggtttt tcaaccagtt ttcgaactgt gcctgccagt gttgtaagtc catacgcgct
    24301 ccgttgccaa agagtcgcca ccttagcagt ttttattcat agcgtctgct acgtgcggga
    24361 cgaggtcggg tgtaccaggc aatgctgcct aacaatgccc cgaccagagc cataaataaa
    24421 aatccgacca gtgcccctaa ccactttccc gcggtagagc ctaaatcgct ttcaaatccc
    24481 gacaccgggg catcagggat ccggctcatc acccagatgt aacgcgccat tgcccagacg
    24541 aaatagccac ccatgtatag aaagcccaca aagccaattt gccgccaggg ctgcgagaga
    24601 gtctctcttc catctttgtc gaaccgtatt ccattttgag ataacccgga aaattcagac
    24661 ttagtactat tatgtgataa aagtcacatt tttccacatt ggatgagcca gtcaagactc
    24721 actcattttt tgaacaaata gataattttt gcgcaacggc agtgaataca ggaaaaatcg
    24781 ttttga
//
LOCUS       DBANUU010000069        24481 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000069 DBANUU010000000
VERSION     DBANUU010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.069"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            97..726
                     /gene="yqiJ"
                     /locus_tag="ACIE68_002690"
     CDS             97..726
                     /gene="yqiJ"
                     /locus_tag="ACIE68_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFX5908982.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            753..2414
                     /gene="yqiK"
                     /locus_tag="ACIE68_002691"
     CDS             753..2414
                     /gene="yqiK"
                     /locus_tag="ACIE68_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFX5908983.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            2653..2712
                     /locus_tag="ACIE68_002692"
     CDS             2653..2712
                     /locus_tag="ACIE68_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFX5908984.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            3029..3088
                     /gene="ibsE"
                     /locus_tag="ACIE68_002693"
     CDS             3029..3088
                     /gene="ibsE"
                     /locus_tag="ACIE68_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFX5908985.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3208..4641)
                     /gene="hldE"
                     /locus_tag="ACIE68_002694"
     CDS             complement(3208..4641)
                     /gene="hldE"
                     /locus_tag="ACIE68_002694"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFX5908986.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(4689..7529)
                     /gene="glnE"
                     /locus_tag="ACIE68_002695"
     CDS             complement(4689..7529)
                     /gene="glnE"
                     /locus_tag="ACIE68_002695"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFX5908987.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SISAHPEWLTELESQPPQADEWQHYVAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGEIGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAELHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(7552..8853)
                     /gene="ygiF"
                     /locus_tag="ACIE68_002696"
     CDS             complement(7552..8853)
                     /gene="ygiF"
                     /locus_tag="ACIE68_002696"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFX5908988.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9095..9715
                     /gene="ygiM"
                     /locus_tag="ACIE68_002697"
     CDS             9095..9715
                     /gene="ygiM"
                     /locus_tag="ACIE68_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFX5908989.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWLPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            9779..11017
                     /gene="cca"
                     /locus_tag="ACIE68_002698"
     CDS             9779..11017
                     /gene="cca"
                     /locus_tag="ACIE68_002698"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFX5908990.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(11107..11928)
                     /gene="bacA"
                     /locus_tag="ACIE68_002699"
     CDS             complement(11107..11928)
                     /gene="bacA"
                     /locus_tag="ACIE68_002699"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFX5908991.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12018..12386)
                     /gene="folB"
                     /locus_tag="ACIE68_002700"
     CDS             complement(12018..12386)
                     /gene="folB"
                     /locus_tag="ACIE68_002700"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFX5908992.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            12491..13108
                     /gene="plsY"
                     /locus_tag="ACIE68_002701"
     CDS             12491..13108
                     /gene="plsY"
                     /locus_tag="ACIE68_002701"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFX5908993.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13121..14053)
                     /gene="ttdR"
                     /locus_tag="ACIE68_002702"
                     /gene_synonym="dan"
     CDS             complement(13121..14053)
                     /gene="ttdR"
                     /locus_tag="ACIE68_002702"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFX5908994.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            14260..15171
                     /gene="ttdA"
                     /locus_tag="ACIE68_002703"
     CDS             14260..15171
                     /gene="ttdA"
                     /locus_tag="ACIE68_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFX5908995.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15168..15773
                     /gene="ttdB"
                     /locus_tag="ACIE68_002704"
     CDS             15168..15773
                     /gene="ttdB"
                     /locus_tag="ACIE68_002704"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFX5908996.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            15821..17284
                     /gene="ttdT"
                     /locus_tag="ACIE68_002705"
     CDS             15821..17284
                     /gene="ttdT"
                     /locus_tag="ACIE68_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFX5908997.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17327..18340)
                     /gene="tsaD"
                     /locus_tag="ACIE68_002706"
     CDS             complement(17327..18340)
                     /gene="tsaD"
                     /locus_tag="ACIE68_002706"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFX5908998.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            18578..18793
                     /gene="rpsU"
                     /locus_tag="ACIE68_002707"
     CDS             18578..18793
                     /gene="rpsU"
                     /locus_tag="ACIE68_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFX5908999.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            18904..20649
                     /gene="dnaG"
                     /locus_tag="ACIE68_002708"
     CDS             18904..20649
                     /gene="dnaG"
                     /locus_tag="ACIE68_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFX5909000.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            20844..22685
                     /gene="rpoD"
                     /locus_tag="ACIE68_002709"
     CDS             20844..22685
                     /gene="rpoD"
                     /locus_tag="ACIE68_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFX5909001.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHATAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(22764..23270)
                     /gene="mug"
                     /locus_tag="ACIE68_002710"
     CDS             complement(22764..23270)
                     /gene="mug"
                     /locus_tag="ACIE68_002710"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFX5909002.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            23395..23470
                     /locus_tag="ACIE68_002711"
     tRNA            23395..23470
                     /locus_tag="ACIE68_002711"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23428..23430,aa:Ile,seq:cat)
     gene            complement(23524..24288)
                     /gene="nfeF"
                     /locus_tag="ACIE68_002712"
                     /gene_synonym="yqjH"
     CDS             complement(23524..24288)
                     /gene="nfeF"
                     /locus_tag="ACIE68_002712"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFX5909003.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELVID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLEM
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
BASE COUNT         5931 a         6693 c         6228 g         5629 t
ORIGIN      
        1 ttctctaaca tgtgttatta ttaacaaaga taaatgtaag caaggaaact atcccaaagt
       61 aaatattgcg tcactaaatg gacattggag tgacatatga ttttattcgc cgactataat
      121 accccttatc ttttcgctat ttcctttgta ctgttgatcg gccttttaga aattttagcg
      181 cttatctgcg gtcacatgct ctccggtgct cttgatgcac atcttgacca ttatgattcc
      241 ataaccacag gtcatattag ccaggcactt cattatctca atatcggaag attgccagct
      301 ctcgtcgttc tctgcttgct ggctggtttt tttggtctta ttggcatcct gttgcagcat
      361 gcctgcatca tggtctggca atcaccactg tcgaacctgt tcgtggttcc tgtcagttta
      421 ctgtttacga ttattgcggt gcattatacc ggtaaaatag ttgcgccctg gattccacgc
      481 gaccatagtt cagctattac agaagaagaa tatattggta gcatggcatt aattaccggt
      541 catcaagcca cttcaggtaa tccctgcgaa ggaaaactca ccgatcaatt tggccaaata
      601 cattatttac tactggagcc tgaagaagga aaatttttca ctaaaggaga caaagtgtta
      661 attatttgcc gactttctgc aacgcgatat ttggcggaaa acaatccctg gccacaaata
      721 ttgtaattta ttaatcaaaa ggaaatttaa taatggatga tattgttaat tctgtgccct
      781 cctggatgtt taccgcgatt attgccgtat gcattctgtt tattattgga attattttcg
      841 ccaggctcta tcgtcgcgct tcggcagagc aagcttttgt tcgtactggt ttaggtgggc
      901 aaaaagtggt gatgagcggt ggcgcaatcg tgatgccgat ctttcatgaa ataatcccca
      961 tcaatatgaa tactctgaag ctggaagtca gccgctcaac cattgatagc ctaattacga
     1021 aagatcgtat gcgtgtcgat gtagtagtcg ctttctttgt acgggtaaaa ccgtctgtag
     1081 aaggcatcgc cactgcggcg cagacgttgg ggcaacgcac gctatcacca gaagacttac
     1141 gtatgttggt tgaagataaa tttgtcgatg ccctccgtgc aacagctgca caaatgacca
     1201 tgcatgagtt acaggatacc cgcgagaact tcgtgcaggg agtacaaaat actgtcgcag
     1261 aagacctgtc gaaaaacggt cttgagctgg aaagcgtttc acttaccaac tttaaccaga
     1321 cctcgaaaga acatttcaat ccgaacaatg cctttgacgc cgaaggttta accaaactga
     1381 ctcaggagac ggagcgccgt cgtcgcgaac gtaacgaagt tgaacaggat gtagaagttg
     1441 cggtgcgtga gaaaaaccgt gatgcacttt cgcgcaagtt ggagattgaa caacaagaag
     1501 cgtttatgac gcttgagcag gagcagcagg ttaaaacccg tactgccgaa cagaatgcac
     1561 gtattgcggc ttttgaagct gaacgtcgtc gtgaagcaga gcagacgcga attctggctg
     1621 aacgacaaat tcaggaaacg gagatcgagc gcgagcaggc cgtccgctca agaaaggttg
     1681 aggctgaacg tgaagttcgt attaaagaga tcgaacaaca gcaggttacc gaaatcgcca
     1741 accagacgaa atcgatcgct attgccgcca aatcggaaca acagtcccag gcagaagcgc
     1801 gtgctaacct tgcactcgca gaagcagtaa gtgcgcaaca aaacgtagaa accactcgcc
     1861 agaccgcaga agccgatcgt gctaaacaag ttgccctaat cgctgccgcg caggatgcag
     1921 aaaccaaagc ggttgaactg accgtgcggg cgaaagcaga aaaagaagcc gcagagatgc
     1981 aggcggcggc tatcgttgag ttagccgaag ctacacgtaa aaagggtctg gcggaagcag
     2041 aagcacaacg tgcgctgaac gatgctatca acgtactttc tgatgaacaa accagcctta
     2101 aattcaaact ggccttgttg caggcgctac ctgcggtaat agaaaaatcc gttgagccga
     2161 tgaaatctat tgacggtatc aagattattc aggtcgatgg tctgaatcgt ggcagcgctg
     2221 cgggtgatgc aaacacgggt aatgtggggg gcggaaacct ggcggaacaa gcattatcag
     2281 ccgctctctc ttaccgcaca caggcaccgc tgattgactc cttgctcaat gaaattggcg
     2341 tttcaggcgg ctcactggcg gcattgactt cacccttaac ctcaacaact cccgtcgccg
     2401 aaaacgtaga ataaattttc agctatgaac gtccctggca caaaaagccg gggatttttt
     2461 atatctgcgt tccgctaaaa actgcaaatg atcaggtcgt tgcaacgttt tgcgtgaccg
     2521 ctcggggaag gcaaaactgc ctctgggaaa gcgttgcgcg aggtccggcg ctcatcaaca
     2581 atcgggaagt cagcaaaggg ctgaaacggg aaagcccctc ccgaggaagg ggccttacag
     2641 aaggaaaggg ttatgatgaa gctcgtcatc atactgattg tgttgttaat cgtaagtttc
     2701 gcagcttatt aacagccaat cagagggggg agaaatcctc cctcaccctt gttcctttac
     2761 tttaggttga aaaaaaatca gcgtcaagaa ggtttcatta acacatatcc ctggcccaaa
     2821 aagccgggga ttttttatat ctgcgttccg ctaaaaggtg caaatgctca ggacgttgca
     2881 gcgttttgcg tgaccgctcg gggaaggcaa aactgcttca gggaaagcgt tgcgcggggt
     2941 ccggcgctca tcaacaatcg gggagtcagc aaggggctga aacgggaaag cccctcccga
     3001 ggaaggggcc ttatagaagg aaagggttat gatgaagttc gtcatcatac tggttgtgtt
     3061 gttactgtta agtttcccga cttactaaca actgatcaga ggggggagaa atcctccctt
     3121 acccttgttc ctttacttta ggttgaaaaa acaacagcgt caataggcct gcctgctacg
     3181 aagcgagatc tgtgaaccgc tttccggtta gcctttttta tcctgttgga tcttcttgat
     3241 gatgttagtg gtcgagcaac cgtcttcgaa gttgagcacc agcacttcgc cgccgttggc
     3301 ccagacttct ttactcccgg cgatctcttc tggtttatag tcgccgccct tcaccagtaa
     3361 atctggcagg atcccggcga tcaagcgctg cggcgtgtct tcttcaaacg acaccaccca
     3421 gtcgaccgct tccagtgcgc ccagcacaat catacgctgt tcgagcgggt taacggggcg
     3481 ggaatcccct ttcagccgtt tggtggaggc atcgctgttc acagcgacaa tcagacggtc
     3541 acccagtttg cgagcatttg ccagatagga gacgtgcccg gcgtgcagga tgtcaaagac
     3601 accgttggtc attaccacct tttcgccgcg tttacgcgct gccgctacag caagcttcag
     3661 ttcctcttcg gtcatcacac caaagccggt atctgcgcgc ccacgcactg cattttccag
     3721 ctcgatcggc gaaaccgtgg aggttcccag tttgccgacc accacgccag ccgccgcatt
     3781 ggcaaagaag caggcttctt ccagcgaatt acccgctgcc agcgttgccg ccaggacgcc
     3841 aatcaccgtg tcgcccgcac cggtaacgtc atacacttcc tgcgcctggg ttggcatatg
     3901 cagcggcgct ttacccggtt gcagcagcga cataccctgt tcggaacggg tcactaacag
     3961 agccgagagt tcgtaatcgg caatcagttt catgccgcgc tcaacaatct cttcttcggt
     4021 cttacattta ccgacaacag cttcaaattc cgagagattc ggcgttaaca gcgtagcgcc
     4081 gcggtagcgc tcaaaatcgg taccttttgg atcaatcagc accggaacac ccgctttacg
     4141 cgccagttgg atcatctgct gtacgcttgc cagcgcacct ttggcgtagt cagaaagcac
     4201 cagcgcgcca atcgaactca gcgcctgatt aatccgctcg tgcagcggct gcggatcaac
     4261 accttcgaaa ccttcttcaa aatccagacg gatcagctgt tggttgcggg aaagtacccg
     4321 taatttggta atggtcggat gcgtcggtac agaaacgaag tcgcatttga cgttgacgtc
     4381 ggccagagat ttactcagcg cgcgcgctgc atcgtcaatg cccgtcaacc cgaccaggcg
     4441 tgcattagca ccgagagaag cgatattcat cgccacgtta gccgcgccgc ccggacgttc
     4501 ttcgatggta ttcactttaa ccacgggcac cggcgcttcc ggcgagatac gactggtggg
     4561 gccgtaccag taacgatcca gcatcacatc accaaccacc atcactcctg cacgttcaaa
     4621 ctctggcagc gttactttca ttcctgtctc ctgagagatt caaaatttgc gcgcgataat
     4681 accatacttc attcttccac cagccacttc tgccagcttg cccgcaccag ttcacgctct
     4741 gcggtgaagc aatcctccga cacatggccc ggcaattcct gtaatgccag atgatgaagt
     4801 tcatcgcgaa gcgtagtgta agcacgggtc agcgccatcg cttcctgctc ttccataatg
     4861 tcgttttgcg ccagtagttc cagaatacgc acgttgtctg accagcgcgt taacttcggt
     4921 ttttcatgag cgtagcgcaa caccagatat tgggtaataa attcgatatc ggtaattccc
     4981 ccttcatcag ctttgatatc aaagcgatcg cgatgtttat tgccgagatg agcgcgcatt
     5041 ttctcgcgca tttcccgcac ttccgtttgc agagttttac cttcacgcgg cagcgtcata
     5101 atctcgcgac gcactgcgtc aaagtgcgcg gtgagctgcg gatcgccgta cactacacgc
     5161 gcacgcacca gcgcctgatg ttcccacgtc caggcctcgt ttttctgata atcggcaaat
     5221 gcttctgcgg atgtcaccag cattcccgcc gccccggacg gacgcagtcg agcatccact
     5281 tcatacaaaa tgccggaaga ggtacgcgta ctgaacagat gcataatgcg ttgcgccaga
     5341 cgcagataaa actgccgccc gtcgatttcc cgctcaccgt cagtcatcgc atccattggg
     5401 caatcatgga ggaagataag gtcaagatcg gaactgtagc ctaactccca gccgcccagc
     5461 ttgccgtagc cgaccaccgc aaaaccacgc ccttcgcgtt cgttcaggtg attcggctta
     5521 ccgtagcggg caaccatttg aacccacgcc tgctgaacga cggcatctat catggcttcc
     5581 gccagccagg ttaagtgatc gctcactttc atcaccggta gcgtaccggc gatatccgct
     5641 gcggcgatgc gtaacagctg cgcctgtttg aactgacgca gcgcctcaag ctgttgctct
     5701 tcgtcatctt ccggcacgcg cagcaaatac tggcgcaact catcgcggta ggcatcggtc
     5761 gccgtcggct ggtaaagggt gtttggatcg agcaattcat ccagcaataa tggataacgc
     5821 gccagctggc tggcaatcat cggcgacgcg gcacacagag aaatcaaatg tttaagcgcc
     5881 gcggggaatt cactgagcaa ttctaaatag gtggtgcggg taacaatccc caccagcaag
     5941 gcggtaatgc gcgacagcgt aacggcagcg tcttcacgcg cacagacatc acttagcaga
     6001 tgcggcatca gatggtcgag cacctgacgt cctcgcggcc cgatggtgcg cttatccagc
     6061 tctttgcgga aatcggcaat cagcgttagc acctgtttgc gatcatcctc gctaagatgc
     6121 gccagcactg gcgtagtgtc atcttcctgc aacgcatcct gccacagctc acgccactgt
     6181 tccgacagcg actcttcctg agtttcactt tcgtcgtcgc caatcaattc attaaacacc
     6241 cggcgcacat tggtcatatg tgcggtcagc gccccggtca attgcggcca gtcagcaaag
     6301 tccatcgccc acgccagccg cgcacgatta agctcatcag aaggaagcgt ctgggtttgt
     6361 tcgtcgttaa tgctttgcag caggttttcc agacgccgca ggaacagata cgccactcgc
     6421 aattgttcag catcgttttc agaaagcaga tgcagctcgg caatggcgct gagcgttggc
     6481 agtaaagagc gcgattgcag cgacggttcg cgtccgccgc gaatgagctg gaacacctga
     6541 acgataaatt caatttcgcg aatgccgcct gcgccgagtt tgatattgtc ggtcaaacca
     6601 cgtcgacgca cttcacgggc aatcatccct ttcatgttgc gcagcgactg aatcacgctg
     6661 aaatcgatgt aacgacggaa aacaaacggg cgcagcatcg cacgcaactc gttagcatag
     6721 acgccttcgc tatcgcccat aatccgcgcc ttgaccatcg cgtaacgctc ccagtcgcgc
     6781 ccctgctcct ggtaataatc ttccaacgcg gcaaagctca gcaccagcgg gccaatttcg
     6841 ccaaacggac gcagccgcat atccacgcga tagacgaagc catcctgcgt tggttgatcc
     6901 agcactttaa tcagccgctg ccccatgcgg gtaaaaaact gcgcgttatc cagttcccgg
     6961 cgtccaccct gcgtacaacc atgttccggc caggcaaaaa tcagatcgat atcagaggag
     7021 aaattcagtt ccccgccgcc cagcttaccc atgcctaaaa tcagcagcgg ttgcgcttcg
     7081 ccctgcgcat tgcacggcgt tccccactcg cggcagcagg cgtcatatag ccagtcacgc
     7141 gccgcaacaa tcagcgtctc cgccagatag ctgagctgct gcaatatgct ctcttcagta
     7201 accagtgcca gcgtttgcgc ccaggcgata cgtaccatga tgcgccgacg gaacagccgc
     7261 agctcgcgca ttaacccggc ttcgtcactc acattactga gcgcctcctg caaccatgca
     7321 acgtaatgct gccattcgtc ggcctgcggc ggttggcttt ccagttccgt cagccactct
     7381 ggatgcgcac ttatgctgtc ctgcacaaaa tcactgaaag taagtactga cttcgcctgt
     7441 gcactaagtg attcctcggc taaaggctct ggcagccgct caacaacggt ctgccagtac
     7501 tgctgtaacg gtgaagagag cggcttcata agcgatttta tccttgcctg attaacgttt
     7561 tccgctgtgc aaccagaacg gttcctgatt gtttgcctca ttacggaaat gttcaatttc
     7621 gatgcgttgc ccggtcgcaa tagcgtgatg cagcccctgc cagttctcca gccaggcttg
     7681 cgcgacgaca ggatcatagt aacccgccag caacagtatt gagtcaatat cacgcgtcag
     7741 gcgtggcaac tggtcacggt agcgatcacc taacggctgg cagaaaacgc ttttcagttc
     7801 agcggcatgg cgggaaagat ggatatcggc aaagcgtttg aaggagtcgc tgattttgcc
     7861 ctgcgctttg gcatctaaaa atggctgcca tgctttgctt accaaccatt cggtcaacgc
     7921 cagcttcgcc attgccgttt cggtagagta gaccgccgtc acggcagaca ccgcagaagc
     7981 aatggtcgcc tcgcattgag tcagcagatc acgtaagtga gtgctcgctt tacgcggcac
     8041 aataccaccg aacagcatca gcgtatgacg gaccaggcta atggctgcca gcacctgttc
     8101 tttcgccgca tcgttgccgc gtacccacag ttcttcatga tactgccatt gcgctaacgc
     8161 cagctcgagc gccgcttcca gcccctgttc cacatcggct tttgccgcaa catgcaaaat
     8221 ggtggtcggt ttgatttcac gcgccggatt gccctgcgcc agatgatagc cacgcgccgc
     8281 tttgctcagg ctgccctggc gtaatccggt ttgcgatacc agttggttcg ccagtttcag
     8341 caccgcgcgc gtgtcgccgc taagcagttc cagttccagc tcacagatag gttcagcaaa
     8401 ttcacccgct ttcacttccc cctggtcgag ggcgatttca atttggctac catcgaccgc
     8461 caccagccat ttttcgcgat aaaaatcggt gctgaacagc ggctgcacgc gggaggcgag
     8521 atcggcgggc aattcgccgt tcggccagac ttccgtcggt aactgcgcca ggtcgagcgt
     8581 cggttcgctc aacgccacgt tatattccgg gcgctgatgt aagccgcctg tcactcttcc
     8641 tgcaactttc atggtcatct catagcgacc gttttcgcca cgaatacgta agcccatatc
     8701 gtgcccacgc agccagttat ccggcgtttc gtagtaaata ttcagcaact gcacggggtc
     8761 atggtgctcg ccgcccagcg tattgagatg gtcacgcaac gcctcaacgg cactgtgatt
     8821 aacaataaac tttaattcga tttcctgagc catggccttt acttatcggt tatgtcacat
     8881 aagtgacgat gaacggcgaa cttaatgcga tcttttttgt cagtagatag tattttgcgc
     8941 caaattgcca tgcaacgggc aatttgacgg gcgtaaaagt ttgaagcagt ggcaagacct
     9001 gacacagagg attccgattg atgtcgaatg ctttgcgtag cgacactgat gccactacta
     9061 tcgttccact ttccatgaca ataacgacag cctgatgcca aaattacgcc tgatcggatt
     9121 aactttactc gcacttagcg cgactgccgt ctcacacgct gaagaaacgc gctatgtttc
     9181 cgacgaactg aatacctggg tccgcagcgg tccgggagat cattatcgcc tcgtgggcac
     9241 ggttaacgcc ggcgaggaag tgaccttatt acaaactgac gccaacacca attatgccca
     9301 ggtgaaagac agctctggcc gtaccgcctg gctcccgttg aaacaactta gcactgagcc
     9361 aagcctgcgc tcccgtgtgc cagatctgga aaatcaggtc aaaaccctga ccgataaact
     9421 caccaatatc gataacacct ggaatcagcg caccgcagaa atgcagcaaa aagtggcgca
     9481 gagcgacagc gtgatcaacg ggttaaaaga agaaaatcag aaactgaaaa acgagctgat
     9541 tgtcgcgcag aaaaaggtcg atgccgccag cgtacagctg gatgacaaac agcgcaccat
     9601 catcatgcaa tggtttatgt atggcggtgg cgtgctgggg cttggcttgc tgctcggtct
     9661 ggtactgccg cacctgatcc caagccgcaa acgcaaagat cgctggatga actaaatcgc
     9721 cttcttcgtt gcactgtctt attattagga attgtgtatt tgacgaaggg atgatggcgt
     9781 gaagatttat ctggtcggtg gtgctgttcg ggatgcattg ttagggctac cggtcaaaga
     9841 cagagattgg gtggtggtcg gcagtacgcc acaggagatg ctcgacgcgg gctaccagca
     9901 ggtaggccgc gattttcctg tgtttctgca tccgcaaacg catgaagagt atgcgctggc
     9961 acgtaccgaa cggaaatccg gttccggtta caccggtttt acttgctatg ccgcaccgga
    10021 tgtcacgctg gaagatgatc ttaagcgtcg cgatctgacc attaatgcgc tggcccagga
    10081 cgataacggc gagattatcg acccgtacaa cggtctgggc gatctgcaaa atcgtctgtt
    10141 gcgccatgtt tcccccgctt ttggcgaaga tccgttacgc gtattgcgcg tggcgcgttt
    10201 tgctgcgcgt tatgcccacc tcggttttcg tattgccgat gaaactctgg cgttgatgcg
    10261 cgagatgacc catgcgggtg aactggaaca cctgacgcct gaacgggtat ggaaagagac
    10321 ggaaagcgcc ctcaccaccc gcaatccaca ggtgttcttc caggtactgc gcgattgcgg
    10381 cgcactgcgc gttttattcc cggaaattga cgcactgttt ggcgttccgg cccctgccaa
    10441 gtggcatccg gaaatcgata cgggtattca taccttaatg acgctctcaa tggcggcgat
    10501 gctgagtccg caggtcgatg tccgtttcgc aactttatgc cacgatctcg gtaaagggct
    10561 gacgccgcca gaactctggc cgcgtcatca tggtcatggc ccggcgggtg ttaagttagt
    10621 ggaacaacta tgccagcgtc tgcgcgtgcc aaatgaaatt cgcgatttag ccagactggt
    10681 ggctgagttt cacgatctca tccacacctt cccaatgctg aacccaaaaa ccatcgtcaa
    10741 attatttgat tccatcgatg cctggcgtaa accgcagcgt gtcgagcaac tggcgctgac
    10801 cagcgaggct gacgtgcgcg gcagaaccgg ttttgaatca gcggactacc cgcaaggccg
    10861 ctggttgcgc gaagcctggg aagtggcgca gtcagtgccg acaaaagccg tcgttgaagc
    10921 gggatttaaa ggtgtggaga ttcgcgagga gctgacccga cggcggattg cggcggtagc
    10981 cagctggaag gaacaacgtt gcccaaagcc tgaatgagta ttgggcattt tgccggatgc
    11041 ggcgtgaacg ccttattcgg cctacgcaat cgttgacaac gccaagcatc cgacactatt
    11101 cctcaattaa aagaacacga catacaccgc agccgccaca ataaagcgat aaatggcgaa
    11161 cgggataaac gaaatgcgct taatcaattg caggaaggtt ttaatcgcta tcagcgccac
    11221 cacaaaagcg gtgataaacc caacggcaaa catcgggata tcgccgcttg tcaggaagcc
    11281 ccagcttttg tagagatcga gcgccgttgc gcccatcatc atcggcaccg ccagcaggaa
    11341 cgaaaactcg gaagcagcgt aacggctcac ccccatcagc atcccacctg aaatggtcgc
    11401 cccggaacgg gagaaacccg gccacagcgc cagacactgg aaacagccaa tcataaatgc
    11461 ctgacgatag gtcatatcat caagacccgg cgcacgcggc tctttcggct tcaggcattc
    11521 ggcggcaatc agcaacaaac cgccaacgac cagcgcatac atcacattta tcgggttaaa
    11581 caatgactta atcgtgtcgt ggaacaacag ccccaatacc accgccggaa tcatccccag
    11641 caaaatgtgg atcagcgtta aacgaccttt gctttcacct tcgtgctgca acgggcggcc
    11701 aaagtggatg ccaatcaggc caaacagacg ccgccagaac atcactacta ccgccagaat
    11761 tgatcctaac tggatcacaa cttcaaaggt tttcgccgtg tcgccctcaa accccaacaa
    11821 gtgaccgaca ataatcatat ggcccgtgct ggataccggc agaaattctg tcaatccttc
    11881 gaccacaccc aatattgccg ctatcagcag cgagtgcata tcgctcatca ataaacccct
    11941 aaattattaa aatgtaccgc ttgtccgaac tactgcgtat gaccaggtta taaccgtttg
    12001 gtttaacagc tgtaaaatta attattttct ttcagattat tgccacgctc aatgattacg
    12061 ccaacattcg ccgcccgcgc cactgcgcct ggcttgctga gtttgatacg cacccacggc
    12121 gagttgaagc gtgctaacag cagctccgcc acctcttcag ccacgcgttc caccagcgca
    12181 aaacgcgccc cctcgacgtg gctgaccacc gtttctgcaa tgtcagcgta actgaggcaa
    12241 tccgccacat catcactttt cgccgcttta cggttatccc atgccatttc gatatcgaac
    12301 actaacttct gttcgatggt ctgttcccag tcgtaaacac caatagtggt gattaccgaa
    12361 agttgctcta taaatacaat atccatcacg tcctgcctgc tttttggcta accggatacc
    12421 acttccggcg aaatgtgcgt attatccaca gattaatcgt tgaacacgaa ttttcaaaac
    12481 ggaacagctt atgagtgcaa tcgcgcctgg aatgatcctc atcgcgtacc tctgcggctc
    12541 catttccagt gccattctgg tttgccgctt gtgtgggctg cccgatccgc gaaccagcgg
    12601 ctccggcaat ccaggcgcaa ccaatgtgtt acgtatcggt ggcaagggag cagccgtagc
    12661 agtactgatt ttcgacgttc tgaaaggaat gttgcccgtc tggggcgcgt atgaattagg
    12721 tgtcagcccc ttctggctag gcttaattgc catcgccgcc tgtcttggac acatctggcc
    12781 cgttttcttc ggatttaaag gaggaaaagg cgttgctacc gcttttggtg ccatcgcacc
    12841 cattggctgg gatctcaccg gagtaatggc gggaacctgg ttactgaccg tgctattgag
    12901 cggatactcg tcgctgggag cgattgtcag tgcactgatt gctccgtttt atgtctggtg
    12961 gtttaagcca caattcacct tcccggtttc gatgctctct tgcctgatcc tgctgcgtca
    13021 tcatgacaac atccagcgtc tgtggcgtcg tcaggagaca aaaatctgga cgaaattcaa
    13081 aagaaagcgc gaaaaggatc ccgagtgatt tctggtggat ctacatgacc tgatagcctt
    13141 catcgggctt gcccagccgt tgctggcacc atgccgccag aaattccacg cagacacgta
    13201 atttcatgct gcgatagagc ggctcccggt aaacagccca gatattggcg ctttgtgcat
    13261 actccggcaa tacttgcacc agtttgccac tctccagaaa cggcaacaca tcccactcgg
    13321 aacgcagcat aatccctttg ccctccagcg cccattgcaa cactatctcg ccgctattgg
    13381 aggaaagatg cccgctaacc ttcaccgatt ttttctcctg accgttcccc aactcccata
    13441 ttccgtgggt catatcgcgc tctttggtca ccagacagtc atgacgactt aattcctgta
    13501 atgattgtgg ctgcggatat ttttgcagat actcaggtgc tgcgcataat attcttttat
    13561 ttttcgttaa cagatgcgca atataataat caggaatttc gtcattaata cgaatatcca
    13621 gatcaatatt atcctgcact aaatcaattt gccgatcgaa caattcaaaa tgcacctgta
    13681 actcaggata attgcgcata agttcggtaa tggctggcgc aatatggctg cgcccaaaac
    13741 caaaactaca gccaatacga atcatcccct ccgggcgcgt tttgatttgc gtgacgtcat
    13801 cgaccagtcg ctgatattgg gtgaggattt ccagcgcatg ttcgtaacaa cgttggccac
    13861 tttcagtcag cgccacgccg cgagcggagc ggttcagcag cgttgttgcc agcgtatttt
    13921 caaggatttg gatccgcttg gtgacaaatg caggcgtctg cccaagcgtc gccgctgcgg
    13981 cgctaaaact cccgctgtgg acgatttcta ccagtacctg caagtctttg gctaaaggcc
    14041 agctattcag catcgttaat tatccgcagt tgtgataagc gcagtgtatt tcgcaaaaca
    14101 ttgccattcc ctgctctggc gcaatattca ttcttatttt cgcgttgatt cacgaactgt
    14161 taattacata ttcacggtgg caaaaaatat aaaaccacat ttttagtggt agtttgtggc
    14221 ggtgaatttt tccagacaaa tacaaaaact ggagttgcca tgatgagcga aagtaataag
    14281 caacaggcag tgaataagtt gacagagatt gtcgctaact ttaccgccat gatttctacc
    14341 cgaatgcctg atgacgtggt ggataaacta aaacagctaa aggatgccga aacgtcgtcg
    14401 atggggaaaa ttatctacca tacgatgttc gacaacatgc aaaaagcgat tgacctgaat
    14461 cgtcctgcct gtcaggacac cggggagatt atgttcttcg ttaaagtcgg ttcccgcttc
    14521 ccactgcttg gcgagctgca aagcatactc aaacaagccg tggaagaggc aaccgtcaaa
    14581 gcgccactac gtcacaatgc ggtagaaatt tttgacgaag taaacaccgg caaaaatacc
    14641 ggtagcggcg taccgtgggt cacctgggac atcatccccg acaatgacga tgcggaaatc
    14701 gaagtttaca tggcaggcgg cggctgcacg ctacctggcc gctcgaaagt gttaatgccg
    14761 tcagaaggct acgaaggcgt ggtgaaattc gtcttcgaaa atatctccac cctcgccgta
    14821 aacgcctgtc caccggtact ggtgggcgtg ggcatcgcca cctcggtgga aaccgccgcc
    14881 gtactctcgc gtaaagccat tttgcgcccg attggcagcc gccatcccaa tccaaaagcg
    14941 gcagaactgg agctacgcct ggaagaagga ctcaaccgtc tggggattgg tccacaaggg
    15001 ctgaccggca acagttcagt gatgggcgta catatcgaat ctgccgcccg ccatccgtca
    15061 accatcggcg ttgctgtctc taccggctgc tgggcgcatc gtcgcggcac gctgctggtt
    15121 catgccgatc tcacctttga aaatctgtct cacacccgga gcgcgttatg aaaaagatcc
    15181 tgacaacccc gatcaaagct gaagatctgc aagatattcg cgtcggcgat gtgatctacc
    15241 tgaccggtac gctggtgacc tgccgcgacg tttgtcaccg ccgtttgatc gaactgaaac
    15301 gtccgatccc ttacgatctc aacggcaaag cgattttcca cgctggcccc atcgtgcgca
    15361 aaaacggcga caaatgggag atggtctccg tcggcccgac aaccagtatg cgtatggaaa
    15421 gttttgaacg tgaatttatt gagcagaccg gcgtgaaact ggtggttggc aaaggtggta
    15481 tggggccgct gaccgaagaa ggctgccaga aattcaaggc gctacatgtg attttcccgg
    15541 caggctgcgc ggtgctggcg gcaacccagg tggaagagat tgaagaagtg cactggacag
    15601 agctcggaat gccggagtca ctgtgggtct gccgggtcaa agagttcggc ccgctgattg
    15661 tctctattga tacccacggc aacaacctga tagccgaaaa caaaaagctg ttcgccgaac
    15721 gccgcgatcc catcgtggaa gagatctgcg agcacgtcca ttacatcaaa taaccctccc
    15781 ggagaggctc acccctctcc tttttcgcag gcataacacg atgaaacctt ccactgaatg
    15841 gtggcgatat cttgcgccgc tggcggtcat cgccattatt gctctacttc cggttcccgc
    15901 agggctggag aaccatacct ggctctactt tgccgttttt actggcgtga tcgttggact
    15961 gatcctcgaa cccgtgccgg gtgccgtggt ggcgatggtg ggtatatcca tcatcgccat
    16021 actctctccc tggctgctgt tcagcccgga gcagctcgct cagccaggct ttaaattcac
    16081 tgcaaaatcc ctctcgtggg ccgtttccgg tttttctaat tcggttatct ggctgatttt
    16141 cgccgccttt atgtttggca caggctatga aaaaaccggg cttggacgcc gcattgcgct
    16201 gattctggtg aaaaagatgg gacatcgcac gctgtttctc ggctatgcgg tgatgttctc
    16261 cgagctgatc ctcgcacctg taacaccgtc caactcggcg cgtggtgcgg ggattatcta
    16321 tcccatcatc cgtaacctgc caccgctcta tcaatcacaa ccaaacgaca gcagttcgcg
    16381 cagcattggc tcgtacatca tgtggatggg gattgttgcc gactgcgtga ccagcgccat
    16441 tttcttgacg gcgatggcac caaacttgct gttaattgga ctgatgaaaa gcgcatctca
    16501 cgccacgctg agttggggcg actggttcct cgggatgttg ccgctcagca ttttactggt
    16561 tctgctggtt ccctggctgg cttacgtgct gtacccgccg gtactgaagt ctggcgatca
    16621 ggtgccgcgc tgggcagaga cggaactgca ggcaatgggc ccgctctgtt cgcgtgaaaa
    16681 acggatgctg gggctgatgg taggcgcgct ggtgctgtgg attttcggcg gtgattatat
    16741 cgatgccgcg atggtcggtt acagcgtggt ggcactgatg ctgcttctgc gcattatcag
    16801 ttgggacgac attgtcagta ataaagcggc gtggaacgtt ttcttctggc tggcctcgct
    16861 tatcaccctc gccaccggac tcaacaacac cggttttatt agctggtttg gcaaactgtt
    16921 agcaggcagc ttaagcggtt attcgccaac gatggtgatg gtggcgttga ttgtggtgtt
    16981 ttatctactg cgctactttt tcgccagcgc cacggcgtat acctccgctc tcgcaccgat
    17041 gatgattgcc gctgcgctgg cgatgccgga aatcccgctg ccggtgttct gcctgatggt
    17101 tggtgcggca attggtctgg ggagcattct tacaccatac gccaccggcc ccagtccgat
    17161 ttactacggc agtggttatc tgccaacggc ggattactgg cgactggggg cgatttttgg
    17221 gctgatattc ctcgtattgc tggtgattac cggcttactg tggatgcccg tggtgttgct
    17281 ttaagtgagg acggtgccgg aaattgctct ccggcaccca atagttttac gcagccggta
    17341 actccgccag cggccagcgc ggacgcacgc taacgccgag atccgccgtc gcgcctgctt
    17401 taaaccgcac cattccggca taggcgatca tcgcgccgtt atcagtacaa aattccggac
    17461 gcgcgtagaa cacttcgccg cggcgttttt tcatcatttc agccagcttc gcccgtaacg
    17521 tacggttagc actcacgccg cccgccatga ccagtcgctt aaagcccgtc tgatccagcg
    17581 cccgcttgca cttaatcatc agcgtatcga ccaccgcatc ttcaaaggcg cgggcgatat
    17641 cagcacgcgt ctggtcgtcg gtgccgttgt cacgaatggt atttgccgcg aaggttttca
    17701 ggccggagaa gctgaaatcc agccccggac ggtcggtcat cggacgcggg aagacaaagc
    17761 gcccggcagt accctgagcc gccattttcg acagtaacgg cccgccagga taatccagcc
    17821 ccagcagctt cgcggtttta tcaaacgctt ccccggcggc atcatcgata gactcgccga
    17881 gcagctcgta ctgaccaatg ccagtcacgc tgattaactg cgtatgaccg ccggaaacaa
    17941 gcagcgcaac aaacggaaat tccggcgggt tatcttccag catcggcgct aacagatgcc
    18001 cttccatatg gtgtacaggg atcgccggaa cgtcccaggc aaacgccaga gaacgcccca
    18061 cggtcgcgcc aaccagtagc gcgccgacta atccagggcc tgcggtatag gccacagcat
    18121 caatgtcttt tgccgttaaa ccagactcct ttagcgccgc ctggatcaac ggtacggttt
    18181 tacgcacatg atcgcgggag gccagttcag gcacgacgcc gccgtagtca gcgtgcaatt
    18241 tcacctgact atacaattgg ttggctaaca aacctttttc atcgtcgtaa atggcgatgc
    18301 cggtttcatc gcaggaagtt tcaataccca gtacacgcat gacttgtttt acctcgcttt
    18361 attaccgcgc agtgtaggac caatgcgggt tgatgtaaaa ctttgttcgc ccctggagaa
    18421 agcctcgtgt atactcctca cccttataaa agtccctttc aaaaaaggcc gcggtgcttt
    18481 acaaagcagc agcaattgca gtaaaattcc gcaccatttt gaaataagct ggcgttgatg
    18541 ccagcggcaa accgaattaa tcaaaggtga gaggcacatg ccggtaatta aagtacgtga
    18601 aaacgagccg ttcgacgtag ctctgcgtcg cttcaagcgt tcctgcgaaa aagcaggtgt
    18661 tctggcggaa gttcgtcgtc gtgagttcta tgaaaaaccg actaccgaac gtaagcgcgc
    18721 taaagcttct gcagtgaaac gtcacgcgaa gaaactggct cgcgaaaacg cacgccgcac
    18781 tcgtctgtac taattccccg agagcgttgc tctccgatca gaccgagttg tagttgtaag
    18841 gccgtgcttc cgaaaggaat gcgcggctta ttttcgttta tgaattgcta aaaatcgggg
    18901 cctatggctg gacgaatccc acgcgtattc attaatgatc tgctggcacg cactgacatc
    18961 gtcgatctga tcgatgcccg tgtgaagctg aaaaagcagg gcaagaattt ccacgcgtgt
    19021 tgtccattcc acaacgagaa aaccccgtcc ttcaccgtta acggtgagaa acagttttac
    19081 cactgctttg gatgtggcgc gcacggcaac gcgatcgact tcctgatgaa ctacgacaag
    19141 ctcgagttcg tcgaaacggt cgaagagctg gcagcaatgc acaatcttga agtgccattt
    19201 gaagcaggca gcggccccag ccagatcgag cgccatcaga ggcaaacgct ttatcagttg
    19261 atggacggtc tgaatacgtt ttaccaacaa tctttacaac aacctgttgc cacgtctgcg
    19321 cgccagtatc tggaaaaacg cggattaagc cacgaggtta tcgctcgctt tgcgattggt
    19381 tttgcgcccc ccggctggga caacgtcctg aagcggtttg gcggcaatcc agaaaatcgc
    19441 cagtcattga ttgatgcggg gatgttggtc actaacgatc agggacgcag ttacgatcgt
    19501 ttccgcgagc gggtgatgtt ccccattcgc gataaacgcg gtcgggtgat tggttttggc
    19561 gggcgcgtgc tgggcaacga tacccccaaa tacctgaact cgccggaaac agacattttc
    19621 cataaaggcc gccagcttta cggtctttat gaagcgcagc aggataacgc tgaacccaat
    19681 cgtctgcttg tggtcgaagg ctatatggac gtggtggcgc tggcgcaata cggcattaat
    19741 tacgccgttg cgtcgttagg tacgtcaacc accgccgatc acatacaact gttgttccgc
    19801 gcgaccaaca atgtcatttg ctgttatgac ggcgaccgtg caggccgcga tgccgcctgg
    19861 cgagcgctgg aaacggcgct gccttacatg acagacggcc gtcagctacg ctttatgttt
    19921 ttgcctgatg gcgaagaccc tgacacgctg gtacgaaaag aaggtaaaga agcgtttgaa
    19981 gcgcggatgg agcaggcgat gccactctcc gcatttctgt ttaacagtct gatgccgcaa
    20041 gttgatctga gtacccctga cgggcgcgca cgtttgagta cgctggcact accattgata
    20101 tcgcaagtgc cgggcgaaac gctgcgaata tatcttcgtc aggaattagg caacaaatta
    20161 ggcatacttg atgacagcca gcttgaacga ttaatgccaa aagcggcaga gagcggcgtt
    20221 tctcgccctg ttccgcagct aaaacgcacg accatgcgta tacttatagg gttgctggtg
    20281 caaaatccag aattagcgac gttggtcccg ccgcttgaga atctggatga aaataagctc
    20341 cctggacttg gcttattcag agaactggtc aacacttgtc tctcccagcc aggtctgacc
    20401 accgggcaac ttttagagca ctatcgtggt acaaataatg ctgccaccct tgaaaaactg
    20461 tcgatgtggg acgatatagc agataagaat attgctgagc aaaccttcac cgactcactc
    20521 aaccatatgt ttgattcgct gcttgaactg cgccaggaag agttaatcgc tcgtgagcgc
    20581 acgcatggtt taagcaacga agaacgcctg gagctctgga cattaaacca ggagctggcg
    20641 aaaaagtgat ttaacggctt aagtgccgaa gagcgatcgg gaagtccccg acagccgcac
    20701 tgagaggcag cggcaaatat ataagtacgc cctcgtaatt atcgttggcg gtaaacaacc
    20761 gttggatttc agcgttaacg gctgaaggac atcgggtcaa tcgcccaaca ccaacctcat
    20821 gaaataagtg tggataccgt cttatggagc aaaacccgca gtcacagctg aaacttcttg
    20881 tcacccgtgg taaggagcaa ggctatctga cctatgccga ggtcaatgac catctgccgg
    20941 aagatatcgt cgattcagat cagatcgaag acatcatcca aatgatcaac gacatgggca
    21001 ttcaggtgat ggaagaagca ccggatgccg atgatctgat gctggctgaa aacaccgcgg
    21061 acgaagatgc tgccgaagcc gccgcgcagg tgctttccag cgtggaatct gaaatcgggc
    21121 gcacgactga cccggtacgc atgtacatgc gtgaaatggg caccgttgaa ctgttgaccc
    21181 gcgaaggcga aattgacatc gctaagcgta ttgaagacgg gatcaaccag gttcaatgct
    21241 ccgttgctga atatccggaa gcgatcacct atctgctgga acagtacgat cgtgttgaag
    21301 cagaagaagc gcgtctgtcc gatctgatca ccggctttgt tgacccgaac gcagaagaag
    21361 atctggcacc taccgccact cacgtcggtt ctgagctttc ccaggaagat ctggacgatg
    21421 acgaagatga agacgaagaa gatggcgatg acgacagcgc cgatgatgac aacagcatcg
    21481 acccggaact ggctcgcgaa aaatttgcgg aactacgcgc tcagtacgtt gtaacgcgtg
    21541 acaccatcaa agcgaaaggt cgcagtcacg ctaccgctca ggaagagatc ctgaaactgt
    21601 ctgaagtatt caaacagttc cgcctggtgc cgaagcagtt tgactacctg gtcaacagca
    21661 tgcgcgtcat gatggaccgc gttcgtacgc aagaacgtct gatcatgaag ctctgcgttg
    21721 agcagtgcaa aatgccgaag aaaaacttca ttaccctgtt taccggcaac gaaaccagcg
    21781 atacctggtt caacgcggca attgcgatga acaagccgtg gtcggaaaaa ctgcacgatg
    21841 tctctgaaga agtgcatcgc gccctgcaaa aactgcagca gattgaagaa gaaaccggcc
    21901 tgaccatcga gcaggttaaa gatatcaacc gtcgtatgtc catcggtgaa gcgaaagccc
    21961 gccgtgcgaa gaaagagatg gttgaagcga acttacgtct ggttatttct atcgctaaga
    22021 aatacaccaa ccgtggcttg cagttccttg acctgattca ggaaggcaac atcggtctga
    22081 tgaaagcggt tgataaattc gaataccgcc gtggttacaa gttctccacc tacgcaacct
    22141 ggtggatccg tcaggcgatc acccgctcta tcgcggatca ggcgcgcacc atccgtattc
    22201 cggtgcatat gattgagacc atcaacaagc tcaaccgtat ttctcgccag atgctgcaag
    22261 agatgggccg tgaaccgacg ccggaagaac tggctgaacg tatgctgatg ccggaagaca
    22321 agatccgcaa agtgctgaag atcgccaaag agccaatctc catggaaacg ccgatcggtg
    22381 atgatgaaga ttcgcatctg ggggatttca tcgaggatac caccctcgag ctgccgctgg
    22441 attctgcgac caccgaaagc ctgcgtgcgg caacgcacga cgtgctggct ggcctgaccg
    22501 cgcgtgaagc aaaagttctg cgtatgcgtt tcggtatcga tatgaacacc gaccacacgc
    22561 tggaagaagt gggtaaacag ttcgacgtta cccgcgaacg tatccgtcag atcgaagcga
    22621 aggcgctgcg caaactgcgt cacccgagcc gttctgaagt gctgcgtagc ttcctggacg
    22681 attaatcggt aggccggatc aggcgttacg ccgcacccgg cactaggccc tctgcacaaa
    22741 cgccaccttt tcggtggcgt tttttatcgc ccacgcacta ccagcgcctg gtccagctcg
    22801 cgatacgctt caaccagttt ctccagtgaa acgcgactta aaccgctggg atttggcagc
    22861 acccaaatct gcgtcgaacc aatggtgagc gtttgtttcc cccactgtgc accgcgctgg
    22921 ctgaatccct gttcatatgc ttgtttgccc agaatcgcca acgcctgcgg ctgataatct
    22981 tcaatttttt caatcagctt acgcccgcct gcgtgtagct cctgctttga aacttcattg
    23041 gcttgcaccg ttggacggtc taccagtttg gtgacgccac aacgataatc cagcagatgc
    23101 tgtgcctcct gcggcttcaa ctgacggtcg gtaaacccgg cctgatatat caccttccag
    23161 aagcgatttg ccggatgagc aaagggaaaa ccagtcccgg cggatgaaag cccagggttg
    23221 ataccgcaaa acacgacccg taaccctgga gccaaaatat cctcaaccat atttactcct
    23281 ttctaacatc accaggaaaa gtataacgga ttgaaaatac attgtttata aaaacagcag
    23341 gcgcgcggta atggctggat tgcgacacgg agttacttta taatccgcta ccatggcccc
    23401 ttagctcagt ggttagagca ggcgactcat aatcgcttgg tcgctggttc aagtccagca
    23461 ggggccacca gatatagcaa aggctgacga gaaatcgtca gcctttttct ttttatatat
    23521 cagttacttt gcgtgccagt aagccgctgc acgtacccgc tgtgggtcat actgttccgc
    23581 ttcaaagcgg cggcttaaat tcttaacgac tttaccttcg ccggttatcc agatgaagta
    23641 atcatcggca gggatttgca tctgcgccag acgcgcatct accgcctgct catcatgtgc
    23701 cagccattcg atattaaaac catcaaggtg cgcgagataa tcctgacagg cgttatcccg
    23761 cacgctaacc agcgcactaa cttgcggttt aacggcaagt ttgctcaaca tttccaggcg
    23821 gcggcgcaat gcgggcattc cggattcatc gcagacatac agctgatacg cgtaatcttc
    23881 cggcaccacc agcgaaccgc gcggacctgc caccgtaagt ttatcgcccg gttgcgcctg
    23941 catcgcccag ccgctggcga ccccaccgtc gtgaataaag aaatcaatca ccagttcatg
    24001 gcgtagttcg tcatacagcg gcgtatagtc acgcgacggt gggcgtggtc cttccggcca
    24061 gacgatgccc tcttccgtta ccgttggcgg cacaaagtga gcgtcaggtt gaggaaagaa
    24121 gagtttgctg tgatcgtcaa agccacgcga tgtaaaaccg tccagcgcct cgccgccgag
    24181 gacaatgcgc tgaaaaccgg cgctgatgcg ctcaacgcgt aacacagtca gttcacggaa
    24241 gcgcagatca ttgcgaacgc gctgcgggta gcggggggag ttattcattg ttatcgcctt
    24301 cgtgatggta attagatata tctaaataaa actcgcaaat gataatgatt gttaatcatg
    24361 ataaatgcaa gcgatttgta gaactgatat atctgtagtc tgataagacg cgcaacctct
    24421 tatcaagcat cattgctcaa tgccggatgc ggcgtgaacg ccttatccgg cctacatgtt
    24481 g
//
LOCUS       DBANUU010000070        24339 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000070 DBANUU010000000
VERSION     DBANUU010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.070"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..585
                     /locus_tag="ACIE68_002713"
     CDS             <1..585
                     /locus_tag="ACIE68_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFX5909004.1"
                     /translation="DKYSGFLNNYSDLKETTSATGKPVLRWVDPSFDQSKYDSIVWNP
                     ITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIAQRKPVVTTAGPRSLIFRGAITGVD
                     TSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRLYFEGELIDAATNKPVIKVVRQGEG
                     KDLNNESTPMAFENIKQVIDDMATDATMFDVNKK"
     gene            complement(603..1109)
                     /gene="tnpA"
                     /locus_tag="ACIE68_002714"
                     /pseudo
     CDS             complement(603..1109)
                     /gene="tnpA"
                     /locus_tag="ACIE68_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1117..2325
                     /gene="insQ"
                     /locus_tag="ACIE68_002715"
     CDS             1117..2325
                     /gene="insQ"
                     /locus_tag="ACIE68_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFX5909005.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     VGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     AYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            complement(2365..3579)
                     /gene="ydcO"
                     /locus_tag="ACIE68_002716"
     CDS             complement(2365..3579)
                     /gene="ydcO"
                     /locus_tag="ACIE68_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFX5909006.1"
                     /translation="MLRCLYAITYEVTMRLFSIPPPTLLAGFLAVLIGYASSGAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            3632..4168
                     /gene="sutR"
                     /locus_tag="ACIE68_002717"
     CDS             3632..4168
                     /gene="sutR"
                     /locus_tag="ACIE68_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFX5909007.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            4241..6202
                     /gene="rlhA"
                     /locus_tag="ACIE68_002718"
     CDS             4241..6202
                     /gene="rlhA"
                     /locus_tag="ACIE68_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFX5909008.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(6294..6524)
                     /gene="yncJ"
                     /locus_tag="ACIE68_002719"
     CDS             complement(6294..6524)
                     /gene="yncJ"
                     /locus_tag="ACIE68_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFX5909009.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            6746..6900
                     /locus_tag="ACIE68_002720"
                     /pseudo
     CDS             6746..6900
                     /locus_tag="ACIE68_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            6946..7362
                     /gene="hicB"
                     /locus_tag="ACIE68_002721"
     CDS             6946..7362
                     /gene="hicB"
                     /locus_tag="ACIE68_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFX5909010.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            7441..8847
                     /gene="ydcR"
                     /locus_tag="ACIE68_002722"
     CDS             7441..8847
                     /gene="ydcR"
                     /locus_tag="ACIE68_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFX5909011.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            9092..10237
                     /gene="ydcS"
                     /locus_tag="ACIE68_002723"
     CDS             9092..10237
                     /gene="ydcS"
                     /locus_tag="ACIE68_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFX5909012.1"
                     /translation="MSKTFARSSLCALTMTIMNAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            10255..11268
                     /gene="ydcT"
                     /locus_tag="ACIE68_002724"
     CDS             10255..11268
                     /gene="ydcT"
                     /locus_tag="ACIE68_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFX5909013.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGTISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            11269..12210
                     /gene="ydcU"
                     /locus_tag="ACIE68_002725"
     CDS             11269..12210
                     /gene="ydcU"
                     /locus_tag="ACIE68_002725"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFX5909014.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPSVLIALYLAFVKRLGAFDAL"
     gene            12200..12994
                     /gene="ydcV"
                     /locus_tag="ACIE68_002726"
     CDS             12200..12994
                     /gene="ydcV"
                     /locus_tag="ACIE68_002726"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFX5909015.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            13016..14440
                     /gene="patD"
                     /locus_tag="ACIE68_002727"
     CDS             13016..14440
                     /gene="patD"
                     /locus_tag="ACIE68_002727"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFX5909016.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGTAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(14537..14632)
                     /gene="yncL"
                     /locus_tag="ACIE68_002728"
     CDS             complement(14537..14632)
                     /gene="yncL"
                     /locus_tag="ACIE68_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFX5909017.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            14827..15000
                     /gene="ortT"
                     /locus_tag="ACIE68_002729"
     CDS             14827..15000
                     /gene="ortT"
                     /locus_tag="ACIE68_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFX5909018.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            15086..15319
                     /gene="ydcY"
                     /locus_tag="ACIE68_002730"
     CDS             15086..15319
                     /gene="ydcY"
                     /locus_tag="ACIE68_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFX5909019.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(15320..15769)
                     /gene="ydcZ"
                     /locus_tag="ACIE68_002731"
     CDS             complement(15320..15769)
                     /gene="ydcZ"
                     /locus_tag="ACIE68_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFX5909020.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(15766..16284)
                     /gene="mddA"
                     /locus_tag="ACIE68_002732"
     CDS             complement(15766..16284)
                     /gene="mddA"
                     /locus_tag="ACIE68_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFX5909021.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            16465..17502
                     /gene="curA"
                     /locus_tag="ACIE68_002733"
     CDS             16465..17502
                     /gene="curA"
                     /locus_tag="ACIE68_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFX5909022.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPNYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            17700..18365
                     /gene="mcbR"
                     /locus_tag="ACIE68_002734"
     CDS             17700..18365
                     /gene="mcbR"
                     /locus_tag="ACIE68_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFX5909023.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(18401..20503)
                     /gene="pqqU"
                     /locus_tag="ACIE68_002735"
     CDS             complement(18401..20503)
                     /gene="pqqU"
                     /locus_tag="ACIE68_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFX5909024.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTSVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNLGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            20745..21806
                     /gene="yncE"
                     /locus_tag="ACIE68_002736"
     CDS             20745..21806
                     /gene="yncE"
                     /locus_tag="ACIE68_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFX5909025.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(21921..23420)
                     /gene="ansP"
                     /locus_tag="ACIE68_002737"
     CDS             complement(21921..23420)
                     /gene="ansP"
                     /locus_tag="ACIE68_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFX5909026.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            23687..24304
                     /gene="yncG"
                     /locus_tag="ACIE68_002738"
     CDS             23687..24304
                     /gene="yncG"
                     /locus_tag="ACIE68_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFX5909027.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         6232 a         5963 c         6257 g         5887 t
ORIGIN      
        1 gataaatatt ctggtttttt aaacaattac tctgatttaa aagaaacaac ctcagcaaca
       61 ggtaaacctg ttttacgttg ggtagacccg agttttgatc aaagcaaata tgacagcatc
      121 gtctggaacc caatcactta ttatccggta ccaaaacctt ctacccaggt agggcagaaa
      181 gttctggata aaattttgaa ctataccaac accgaaatga aagaagcgat agcgcagcgt
      241 aaaccagtgg ttaccaccgc tgggccgcgt agtctgattt tccgtggggc cattaccggt
      301 gtagatacca gcaaagaagg gctgcaattc tatgaagtgg ttcctgttgc attagtggtt
      361 gcggggacgc aaatggctac aggccaccgt accatggata ctcgcctcta ttttgaaggt
      421 gagctgattg atgcagcgac taataaaccg gttatcaaag tcgttcgtca gggcgaaggt
      481 aaagacctga ataacgaaag tacgccaatg gctttcgaaa atattaaaca agttattgat
      541 gacatggcga ccgatgccac catgtttgac gttaacaaaa agtagttcca gacgcgccat
      601 cgttagatgg cgctttttat ccggtgcgcc gtaaaacccc atccttcagg gcggggatat
      661 aaggcgcggt tttccacctg accaggtgtt tgctgttgtt caatgtattg gcggatgacg
      721 gatattggcg cacctccgca gctactggca aaatagccag gactccacaa aacgcctttg
      781 tagtaatacc tgactgcaat atctggtcga tctcgtcgca gtaacctacc cgatacgcct
      841 ttgaggctgt ttaccagact ggatatcgcc agtttgggag gttagttaat taacaaatgg
      901 acgtgatctg gttcgccatc catttcaacc agttcagctt caaaataagc acatacattt
      961 gaaaagtaag tgcgtagttt ttctgtcgcg tcatggtcaa aaatctgacg tcggtatctg
     1021 gtgacaaaga ccaggtgaac atgcatcagg aaaacacaat gccttccacg ccggatatcg
     1081 gtttcttttt tcacagacca aagtatcatt tggcctgtga aacgactaca agcatttaaa
     1141 ttccagttaa gacccggtgg tcaacaggag cgtgaaatga ggcgcttcgc aggcgcatgt
     1201 cgtttcgttt tcaatcgtgc tctggcacgt cagaatgaga atcatgaggt cggtaataaa
     1261 tacatccctt acgggaaaat ggcttcctgg ctggttgagt ggaaaaatgc cactgaaacg
     1321 caatggctta aagatgcccc gtcacagcca ttgcaacagt cactgaaaga ccttgagcgg
     1381 gcttacaaaa acttcttcca gaatcgggct gcttttcccc gattcaaaaa gcggggacag
     1441 aatgatgtat tccgctaccc gcagggcgtt aagctcgatc aggaaaacag ccgtattttt
     1501 ctgccgaaac tgggctggat gcgctaccgg aacagccgtc aggtcacggg tgttgtgaaa
     1561 aatgtcactg tcagccagtc ctgcggtaag tggtacatca gtattcagac agaaagtgaa
     1621 gtatccactc cggttcaccc ttcagcatca atggtcgggc tggatgctgg cgtggctaaa
     1681 ctcgccacgc tgtcagatgg cacagtcttt gagcctgtaa acagttttca gaaaaaccag
     1741 aagacgctgg cgagacttca gcgacagtta agccgcaagg tcaaattcag caacaactgg
     1801 cagaagcaga aacgcaaaat acagcgactg cattcctgta tcgcaaatat ccgcagggac
     1861 taccttcaca aagtcacaac ggccgtcagc aaaaaccacg caatgatagt cattgaggat
     1921 ttgaaggtca gcaacatgtc aaagtcagca gcgggtacgg tcagccagcc ggggcgcaat
     1981 gtccgggcaa aatcaggttt aaaccgttcg atactggatc agggctggta tgaaatgcgc
     2041 cgccagcttg agtacaagca gctctggcgt ggcggtcagg tgcttgctgt tccgccagcg
     2101 tatacaagcc agcgttgcgc gtactgtggt catacagcga aagagaaccg cctgtcacaa
     2161 agtaaattca gatgccaggt atgtggatat acagcgaacg ccgatgtaaa tggcgctcgt
     2221 aacattttag cggcggggca cgccgttctt gcctgtggag agatggtgca gtcaggccgc
     2281 ccgttgaagc aggaacccac cgaaatgatt caggcgacag cctgaacgta gcagggatcc
     2341 acgtccttca gggcgtggag gatgtcaata tcggtttctg tcagcgatta aattcaacac
     2401 cacgtaacaa acgcctccgg caattaatcc ccagaacgca gaaccaatcc cgaccagcgt
     2461 caatccactt gccgttacca gaaatgccac caccgccgcg tctcgctcac gctcattatg
     2521 cagcgcctga tacaaactgc cgctaatggt acttaacagc gccagacctg ccagcatctg
     2581 gatccaactt acgggcaggg cagccatcat cccggtaata gcactaccaa acagacctgc
     2641 gatcaaataa aaaatgcctg caacggcagc tgccagccaa cgttgatctt tatccggatg
     2701 cgcttccggg ctttggcaaa tagccgcggt gattgccgca ataccgacgg aataaacgcc
     2761 gaaaggggaa aaaaccagtg ccagcaatcc agtaaataca attaatggcg aaacaggagc
     2821 cgaatacccg gctgctttca ttgctgcgat acccggtgcg ttttgcgatg ccatcgtcac
     2881 cagaaaaagg gggagtgcaa cgctcaggct gtgagcaaac gaaaaatcag gggtaatata
     2941 agtggggaga acgggtttaa agacaacatc agttgtgaca acgtcacctt gcgcgataac
     3001 gatcacgatc ccaataatca tcgcggcaat taccgcatag cgcggcgcaa cggccttggt
     3061 tgccagccat accagcaaca tacttccaca caacgtaaat tgaccgtcca gactggcaaa
     3121 cgcctgtaaa ccaaagcgta ataaaatccc ggcaagcatt gccgccgcaa gcgagtgcgg
     3181 aataatgcgc atcagacgag caaagagtcc cgttatgccg cagaggacta ttaacgcgtt
     3241 ggtgacaata aaaacgccga tggcttcgtt aagtgttagt ccctgcaatc cggtgaccaa
     3301 caaagccgcg ccaggcgttg accatgcggt gagaacaggt acgcgatacc ataatgtcag
     3361 agtcagcgta ctgacgccca ttgccagccc cagcgccgtc atccagccag agatttgtgc
     3421 agtggtggct ccggcgacaa tcgctgcttg ccagattatt gcccctgaac tggcgtagcc
     3481 aattaatacc gccagaaacc ccgccagtag cgtgggtgga ggaatagaaa acagacgcat
     3541 agtcacctcg tacgttatag cgtacaggca ccgtagcatt tgtccgttat aacgcacaag
     3601 tgataaactt ccgttttgcc ggaggagtcg catggaaaat ctcgctcgct ttttatccac
     3661 cacacttaaa caactacgcc agcagcgcgg ctggagtctt tcgcgactgg cagaagcgac
     3721 gggcgtttct aaagcaatgt tagggcagat tgagcgtaat gagtccagcc cgacggtcgc
     3781 gacattatgg aaaattgcca ccgggttgaa tgtgccattt tcgacattta tttctccgcc
     3841 acagtccgct acgccttcag tttatgatcc acaacagcag gcgatggtga tcacttcgct
     3901 gtttccttat gatccacaac tctgctttga acacttctcg attcagatgg cacccggtgc
     3961 aataagcgaa tcgacgcccc atgagaaggg ggtaatcgaa catgtcgtcg tcattgatgg
     4021 acaacttgat ctgtgcgttg atggggaatg gcagaccctt aattgtgggg aaggcgttcg
     4081 atttgctgca gacgtcacgc atatctatcg taatggtggg gagcaaaccg tacattttca
     4141 ttccctcatc cattacccgc gcagttaagc gggaaaacta tttcgcaacg tcgcgcttgt
     4201 ggctaaaata gccgccattt ttcagctact ggataagaat gtgaccgtat cttctcatcg
     4261 acttgaactg ttaagcccgg cacgcgatgc cgccattgcc cgcgaagcta ttttgcacgg
     4321 tgccgatgct gtttatatcg gcggccctgg ttttggtgcc cgtcataatg ccagtaatag
     4381 cctgaaagat attgccgagc tggtgccgtt tgcccatcgt tatggtgcaa aaattttcgt
     4441 cacgcttaac accattttgc atgatgatga gctggaaccc gcgcaacggc tgattactga
     4501 cctctaccag accggtgtcg atgcgctgat tgttcaggat atggggattc tggaacttga
     4561 tattccgccg attgaactgc acgccagtac gcagtgcgac attcgtacag ttgaaaaagc
     4621 gaagttcctc tctgatgttg gcttcacgca gattgtgctg gcgcgagagc tgaatcttga
     4681 tcagatccgc gcgattcacc aggctacgga cgcgaccatt gaattcttta ttcatggggc
     4741 actgtgcgtg gcctattcgg gtcagtgcta catttctcat gcgcaaacag ggcgtagcgc
     4801 caaccgtggc gattgctcgc aggcgtgccg tttgccatac acattgaaag acgatcaggg
     4861 gcgggtggtt tcctatgaaa aacatctgct gtcgatgaaa gataacgatc agactgccaa
     4921 cctcggcgcg ctgattgatg ctggtgtacg ctccttcaag attgaagggc gttacaaaga
     4981 tatgagctac gtgaagaata tcaccgccca ttatcgccag atgcttgatg ccattattga
     5041 agaacgtggc gatctggcgc gcgcttcatc aggtcgtact gaacatttct ttgttccatc
     5101 gacggaaaag actttccacc gtggtagcac agattatttt gtgaatgccc gtaaaggcga
     5161 tattggcgcg ttcgattcgc cgaaatttat cggcctgccg gtaggcgaag tattgaaagt
     5221 ggcgaaagat catctcgatg ttgccgttac cgagccactg gcaaatggcg atggcctgaa
     5281 cgtgttgatt aaacgtgaag tcgtcggttt tcgtgccaat acggtcgaga aaaccggaga
     5341 aaatcagtac cgcgtctggc ccaatgaaat gccagcagat ttgcacaaaa ttcgtccaca
     5401 tcacccacta aaccgtaatc ttgatcataa ctggcagcag gcactgacaa aaacctccag
     5461 cgaacgtcgg gtggcggtag acattgaact gggcggctgg caggaacaac tgattctgac
     5521 cctcaccagt gaagagggtg tcagcatcac gcatacgctg gacgggcagt tcgacgaagc
     5581 caataacgcc gaaaaagcaa tgaacaatct gaaggatggt ctggcaaaac tggggcaaac
     5641 cctctattac gcccgcgatg tgcaaattaa tttgccgggg gcgctgtttg taccaaacag
     5701 tctgttaaac cagttccgcc gtgaagctgc tgacatgctg gatgctgcgc gtcttgccag
     5761 ttaccagcgc ggcagccgta aaccggttgc tgatcctgcg ccggtttatc cgcaaacgca
     5821 tctgagtttc ctcgcgaacg tatacaacca gaaagcgcgt gaattttatc atcgctatgg
     5881 tgtgcagctg attgacgcgg cgtatgaagc acatgaagag aagggcgaag tcccggtgat
     5941 gatcaccaag cattgtctgc gctttgcctt taatctgtgc ccgaaacagg cgaaaggcaa
     6001 tatcaaaagc tggaaggcga cgccaatgca actggttaac ggcgatgaag tattaacgct
     6061 aaagtttgat tgccgcccat gcgagatgca cgtcattggc aaaatcaaaa atcacatact
     6121 gaaaatgccg ttaccgggaa gcgtagtggc atccgtaagt ccggatgagc tgctgaaaac
     6181 attgcctaag cgaaaagggt aaaacgccag ttttctggtt actcacaact cattgaatct
     6241 gcatgatatt gcctgccggg taaggcgtca cgccgcatcc ggcaacaaat gactcaacgt
     6301 tgtcccgctt ctggtttgcg cgatttttgc cagtaaaaat gctcgcgcaa accttccgcc
     6361 gactcttccg ccacagcacg caattcatcg ctgtccgctt catgacgcag ctgatgatcc
     6421 acattcttta cccacacaaa ttcatgtcct ttgtgccctg ccatgagttg tcctgagaac
     6481 agcgcacacg ttaataagac aaccgataac gccttcgtaa acatcctgcc acctttttct
     6541 taccttttgc cgctatgatg ccgatcgttt cttgaggtta ttattcagtt ttgcaaatta
     6601 gcgcaaagaa attctggaat cttccttcct gattttgcat tgcattctgc cgttgcggcg
     6661 atttagtgct attttcgagc aaattacaca cagaggtaaa cactaatgtt tactttgttg
     6721 atatactcag cggcagggag gcgatgtgaa acaaagcgag ttcagacgtt ggctcgaatc
     6781 tcagggcgtc gatgtagcga atggccgcaa ccatttgaaa ctcaggtttc atgggaggcg
     6841 cagtgtcatg ccgcgtcact cctgcgtaaa gcaatcctga aacaactcgg tttgagttaa
     6901 tcgccaatta aaaaggttaa tgacatgcga gagacagtcg aaattatgcg ttatcccgtc
     6961 actcttacac ccgcgccgga aggcggttat atggtttctt ttgtggatat ccctgaagcg
     7021 ttgacgcagg gcgaaactgt cgctgaagcg atggaagcgg caaaagatgc tttactgacc
     7081 gcatttgatt tttattttga agataacgag cttatcccgt taccttcgcc attaaatagt
     7141 catgatcact ttattgaagt acctttgagc gtcgcctcta aagtattgct gttaaatgct
     7201 tttttacagt cagaaatcac tcagcaagag ttagccaggc gaattggcaa acctaaacag
     7261 gagattactc gcctatttaa cttgcaccat gcgacaaaaa tcgacgccgt ccagcttgcg
     7321 gcaaaggcgc ttggcaaaga gttatcgctg gtgatggttt aattacagtt aacgaaaagt
     7381 tgtcattttt aacaactgat atagactgcc gaatcatctg cacataatta cgattcgata
     7441 atgaaaaaat accagcagct tgcagaacaa ttacgcgagc agattgcgtc gggtatctgg
     7501 caacccggcg atcgtttgcc ttcgttgcgt gaccaggtgg cgctttcagg catgagcttt
     7561 atgactgtca gccatgccta tcagttgctc gaaagtcagg gatatattat cgcacgaccg
     7621 cagtcgggtt attacgttgc gccacaggca ataaaaatgc cgaaagcgcc agtcattcca
     7681 gtcactcgag atgaagcagt cgatatcaac acttatattt ttgatatgtt gcaggccagt
     7741 cgcgatccgt cggtcgttcc gtttgcctcg gcctttcccg acccgcgact tttccccctc
     7801 caacaactaa accgctcgct ggcgcaggta agcaaaaccg ccacagcgat gagcgtgatt
     7861 gaaaacttac cgccaggaaa cgcagaactg cgtcaggcta ttgctcgtcg ctatgcctta
     7921 cagggcatca ccatttctcc tgatgaaatt gtcattactg ccggggcgtt agaggcatta
     7981 aacctcagtt tgcaagcggt aactgaaccg ggcgattggg tgatagtaga gaatccttgt
     8041 ttctacggtg cgttgcaggc gctggagcgg ctacggctga aggcgttatc ggtggcgacg
     8101 gatgttaaag aagggatcga tcttcaggcg ctggaactgg cgttgcagga ttatccggtg
     8161 aaagcgtgct ggctgatgac taatagccag aacccactcg gatttacctt aacgccgcaa
     8221 aaaaaagcac aactggtggc gttactcaat cagtacaacg taacgctgat tgaagatgac
     8281 gtttacagcg aactttattt tggacgggaa aaaccgctgc ctgcgaaagc gtgggatcgc
     8341 cacgatggcg ttttgcattg ctcttcgttt tcgaaatgtc tggtgcctgg ttttcgtatt
     8401 ggttgggtcg ccgccggaaa acatgcacgt aaaattcaac gcttgcagtt gatgagtacg
     8461 ctttccacca gctcaccgat gcaacttgcg ctggtggatt acctttccac gcgccgatac
     8521 gacgcccatc ttcgtcgcct gcgtcgccag cttgcggaac gtaaacaacg tgcctggcag
     8581 gcactgctgc gttatctgcc tgcggaagtt aaaattcatc atagtgacag cggttatttt
     8641 ctctggctgg agctccccga gccgttagat gccggcgaat taagcctggc ggcactgacg
     8701 catcatatca gtattgcgcc gggtaaaatg ttttctaccg gtgaaaactg gtcacgtttt
     8761 tttcgtttta ataccgcgtg gcagtgggga gaacgtgaag aacaggcggt aaaacaatta
     8821 ggcaaactta ttcaagaacg gctgtaatag cgtttaattt aattcctctt agattgggta
     8881 atatgaattt cgaatagcag tcatatttcc taaccccttg tctatactcc agaagataac
     8941 cttacagacg gcataatgcg cggtagctca caacctgatt aaattttctc aggggcgaag
     9001 gtgtgcctgc aagccgccgt ctatggttaa acaaggagat atttttacgg cacggcggct
     9061 gaacaattaa ttacgacagg agtaagacct tatgagcaag acatttgccc gcagcagcct
     9121 gtgtgcgctc accatgacaa taatgaacgc tcacgccgcc gaaccaccta ccaatttaga
     9181 taaaccggaa gggcgactgg atattatcgc ctggccggga tacatcgaac gcggacaaac
     9241 tgataaacaa tacgactggg taacgcaatt tgaaaaagag acaggctgcg cggtgaatgt
     9301 gaaaaccgcc gcgacttccg atgaaatggt cagtctgatg accaaagggg gttacgatct
     9361 ggttacggca tccggcgatg cctcgctgcg tttgattatg ggcaaacgcg tgcagccgat
     9421 taataccgcc ttgattccca actggaaaac gctcgatccg cgcgtggtta aaggcgactg
     9481 gtttaatgtt ggcggcaaag tttacggcac accttaccaa tgggggccga acctgctgat
     9541 gtacaacact aaaaccttcc cgacgccgcc ggatagctgg caagtggttt ttgttgagca
     9601 aaatctgccg gacggcaaga gcaataaagg ccgcgttcag gcttatgatg gccctatcta
     9661 cattgcggac gctgcgttgt tcgttaaagc cactcagccg cagttgggca tcagcgatcc
     9721 gtatcaacta accgaagaac agtaccaggc ggtgctgaaa gtgctgcgcg ctcaacacag
     9781 tttgatccat cgctactggc atgacactac cgtgcaaatg agcgatttca aaaacgaggg
     9841 tgtggttgct tccagtgcct ggccctatca ggccaacgcc ctgaaagccg aaggccagcc
     9901 tgttgctacc gttttcccga aggagggtgt taccggttgg gctgatacca ccatgctgca
     9961 tagcgaagcg aaacatccgg tttgcgccta caaatggatg aactggtcat taacgccaaa
    10021 agtgcagggc gatgtggcgg cctggtttgg ctcgttaccg gtagtgccgg aagggtgtaa
    10081 agccagtccg ttattaggcg aaaaaggttg tgaaaccaac ggttttaact atttcgacaa
    10141 aatcgccttc tggaaaacgc ctatagcaga agggggcaag tttgttccct acagtcgctg
    10201 gacgcaggat tacattgcca ttatgggcgg tcgctaactt cgctggggtg ctttatgacg
    10261 tacgcagtgg agtttgacaa cgtctcgcgg ttgtacggtg acgtgcgcgc agtagatggc
    10321 gtcagtattg cgataaaaga tggtgagttc ttctctatgc tggggccgtc cggctccggc
    10381 aaaaccacct gcctgcgcct gattgctggc ttcgaacagc tttccggcgg gactatctct
    10441 atctttggta aacccgccag caatctgcca ccgtgggagc gggacgtgaa tactgtcttt
    10501 caggactacg cgctatttcc gcatatgtcg attcttgaca atgtcgccta tgggctgatg
    10561 gtcaaaggcg tgaataaaaa gcagcggcac gcaatggcgc aagaggcgct ggagaaagtg
    10621 gcgttggggt ttgtacatca acgtaaaccg tcacaacttt ctggtggtca gcgccagcgg
    10681 gttgctatcg ccagagcatt ggtgaatgaa ccgcgcgtat tgctgttgga tgaaccgctc
    10741 ggcgcactgg atctcaaatt gcgtgagcag atgcagttgg aactgaaaaa actgcaacag
    10801 tctctcggta tcacttttat cttcgttacc cacgatcagg gcgaagcgtt atcgatgtcc
    10861 gatcgtgtgg cggttttcaa taatggacgc attgagcagg tcgattcccc gcgcgatctc
    10921 tatatgcgcc cgcgcacgcc gtttgttgcc ggattcgtcg ggacatccaa cgtttttgat
    10981 ggactgatgg cagagaaact ttgtggcatg acgggaagct tcgccctgcg accggaacat
    11041 atccgcctca acacccctgg tgaactgcag gccaatggca cgatccaggc ggtgcaatat
    11101 cagggcgcgg caactcgttt tgaactgaaa ttgaacggcg gtgaaaaact gcttgtgagt
    11161 caggccaata tgacaggcga agaactgcct gccacgctca cgcccggaca acaggtgatg
    11221 gtttcctggt cgcgtgatgt gatggtgccg ctggttgagg agaggtgaat ggcgatgaat
    11281 gtattgcaat caccttcacg tccaggtctg ggtaaggtgt ccggtttctt ctggcgtaat
    11341 ccggggctgg ggctgttttt actgctgctt ggcccgctaa tgtggtttgg cattgtctat
    11401 ttcggctcgc tgctgacact gttatggcag ggattttata cttttgacga tttcaccatg
    11461 tcggtaacgc cggaactgac gctggcgaat atccgtgcgc tgtttaatcc ggcgaattac
    11521 gacatcattc tccgcacgct gactatggct gtggcagtca ctatcgccag cgccattctg
    11581 gcttttccaa tggcgtggta tatggcgcgc tataccagcg ggaaaatgaa agcttttttt
    11641 tatattgcgg taatgttgcc gatgtgggcg agctacattg ttaaagccta tgcctggacg
    11701 ttattgctgg caaaagatgg cgtggctcag tggtttttac aacatcttgg gctggaacca
    11761 ctgctgactg cgttccttac attacctgcg gtgggcggaa atacgctgtc aacttccggg
    11821 ctggggcgct ttctggtgtt tctctatatc tggttgccgt tcatgatcct gcccgttcag
    11881 gcggcgcttg agcgtttgcc gccgtcattg ttgcaggcgt cggctgatct cggcgcacgt
    11941 ccacgacaaa cctttcgcta tgtggtgctg ccgctggcaa tcccgggtat tgccgctggc
    12001 tctatcttta ccttctcact cacactgggc gattttatcg tcccgcagtt ggttggtcct
    12061 ccaggatatt ttatcggcaa tatggtttat tcccagcagg gggcgattgg caatatgccg
    12121 atggcggcgg cattcaccct ggtgccgagt gttctcatcg cactgtacct ggcgttcgtg
    12181 aaacgtctgg gagcgttcga tgcactctga acgcgcaccg tttttcctca aactggcggc
    12241 ctggggcggc gtggttttcc tacattttcc catcctgatt atcgccgctt atgcgtttaa
    12301 cactgaagat gcggcgttta gttttccacc gcagggcctg acgctgcgct ggtttagcgt
    12361 ggcagcacag cgtagtgata ttcttgatgc cgtgacactg tcacttaaag tggcggcgct
    12421 ggcgacatta atggcgctgg tgttagggac gctggcagct gccgcgctgt ggcgacgaga
    12481 ctttttcggc aaaaacgcca tttcgctgtt actgctgctg cccattgcgc tgccgggcat
    12541 tgtcactggt ctggcgctat taaccgcttt taaaaccatc aatctggagc cgggattttt
    12601 caccatcgtg gtcggtcatg cgactttttg cgtagtggtg gtgtttaaca atgtcatcgc
    12661 ccgttttcgc cgcacctcgt ggagtctggt tgaggcgtca atggatcttg gggccaatgg
    12721 ctggcaaacc ttccgctacg tagtgttgcc gaatctcagt tcggcgctac tggcaggagg
    12781 aatgctggcg tttgccttgt cgttcgatga aatcatcgtt acgactttta cggcaggtca
    12841 tgaacgaacg ttaccgttgt ggttgctcaa tcagcttggg cgaccgcgtg atgtaccggt
    12901 aactaacgtg gtggcactgc tggttatgtt ggtaacaacc ttgccgatcc tgggggcctg
    12961 gtggctaacc cgcgaaggcg acaatggtca ataaccactg atacaggaat atgctatgca
    13021 acataagtta ctgattaacg gagaactggt tagcggcgaa ggggaaaaac agcctgtcta
    13081 taatccggca acgggggacg ttttactgga gattgccgag gcatccgcag agcaggtcga
    13141 tgctgctgtg cgcgcggcag atgcagcatt tgccgaatgg gggcaaacca cgccgaaagt
    13201 gcgtgcggaa tgtctgctga aactggctga tgttatcgaa gaaaatggtc aggtttttgc
    13261 cgaactggag tcccgtaatt gtggcaaacc gctgcatagt gcgttcaatg atgaaatccc
    13321 ggcgattgtc gatgtttttc gctttttcgc gggtgcggcg cgctgtctga atggtctggc
    13381 ggcaggtgaa tatcttgaag gtcatacttc gatgatccgt cgcgatccgt tgggggtcgt
    13441 ggcttctatc gcaccgtgga attatccgct gatgatggca gcgtggaaac tggctccggc
    13501 gctggcggca gggaactgcg tagtgcttaa accatcagaa attaccccgc tgaccgcgtt
    13561 gaagttggca gagctggcga aagatatctt cccggcaggc gtgattaacg tactgtttgg
    13621 cagaggcaaa acggtgggtg atccgctgac cggtcatccc aaagtgcgga tggtgtcgct
    13681 gacgggctct atcgccaccg gcgagcacat catcagccat accgcgtcgt ccattaagcg
    13741 tactcatatg gaacttggtg gcaaagcgcc agtgattgtt tttgatgatg cggatattga
    13801 agcagtggtc gaaggtgtac gtacatttgg ctattacaat gctggacagg attgtactgc
    13861 ggcttgtcgg atctacgcgc aaaaaggcat ttacgatacg ctggtggaaa aactgggtac
    13921 tgcggtggca acgttaaaat ctggtgcgcc ggatgacgag tctacggagc ttggaccttt
    13981 aagctcgctg gcgcatctcg aacgcgtcag caaggcagta gaagaggcga aagcgacagg
    14041 gcacatcaaa gtgatcactg gcggtgaaaa gcgcaagggt aatggctatt actatgcgcc
    14101 gacgctgctg gctggcgcat tacaggacga tgccatcgtg caaaaagagg tatttggtcc
    14161 agtagtgagt gttacgctct tcgacaacga agaacaggtg gtgaactggg cgaatgacag
    14221 ccagtacgga cttgcatctt cggtatggac gaaagatgtg ggcagggcgc atcgcgtcag
    14281 cgcacggctg caatatggtt gtacctgggt caatacccat ttcatgctgg taagtgaaat
    14341 gccgcacggt ggacagaaac tttctggtta cggcaaggat atgtcacttt atgggctgga
    14401 ggattacacc gtcgtccgcc acgtcatggt taaacattaa ggataatatt gcagatcgta
    14461 agagtatgag atgatcttgc gtactgtcag agcactgatt tctctgacag tacgtgctgc
    14521 gtgcaaaatc actacatcaa ataaaccagc caaatctcat agagataaga gtaaacaaac
    14581 caacagcagc aaagacattt atcagtacta cagttctact ggaaacattc atttttgcca
    14641 ccttttatca ttgcccctat gtgtatagta cgggttaaga aaatccgcaa taatactcat
    14701 tctgaagatg aataagggca agttgctgtt tgatgatttt ttctcaaact ggtgaaaaaa
    14761 cttgatgcac gtcaaaaaat gacgcatatt tgcgcgcgtt ttattcatct ggctggacgc
    14821 ccgtacatgt ctctctatca acacatgctt gttttttatg cggttatggc agcaatcgca
    14881 tttcttatca cctggtttct ttctcacgat aagaaacgca tccgtttctt aagcgccttt
    14941 ctggtggggg caacatggcc gatgagtttc ccggtggcgc tgttgttttc actgttttaa
    15001 atcttactca ctggcttgac ggcaccacgc agacttatat catttggatg aatcgataaa
    15061 tttcacaagt ggctaaggag aaagtatgtc gcatctggat gaagtcatcg cgcgcgtgga
    15121 tgccgcgatt gaagagagcg tcattgccca tatgaacgaa ttattaatcg ccctgagcga
    15181 tgacgcggag ttaagtcggg aagatcgcta cacccagcag cagcgtttgc gcaccgctat
    15241 tgctcatcac ggtcgcaagc ataaagaaga tatggaagcg cgccacgaac agttaaccaa
    15301 aggcggcacc atcctctgat taaaatgagc gtctggctac caaccaggcg ccaaccacaa
    15361 gtaaaattgc gccacaaatt gggccgaaca acgcccgtag tggtacgcca tgactgcgga
    15421 agatatccag catcagaccg ccaattagct ggctggcgac cagtactgca atagttgtcg
    15481 ccgctcccac attctgatat ccgctgatac tggcgaagac aaaaaacgat cccagcaagc
    15541 cgggtataag tgtccaccag cgcacgctgg acaccagttc gccaaacccc gccatgcctt
    15601 gtttaaacca taaaatagaa acaaagagca caatccccac cagtgagttc agcaacatgg
    15661 cgatgagaat ggtagaggag gtctgggtga tgcgcaccat cagcgtgttc tggactacca
    15721 gaccaattcc ggcggcaatc aaaaaagcaa gggtgagcga ctgattcatc cgatcgcgtc
    15781 cggttcagtg cgttcgtcga gttgcaactg cataaatgtc agatccagcc agcgcccaaa
    15841 tttagtgccc acctgcggca tttgtgcggt gacgacaaat cccagcgaat ggtgcagatg
    15901 cagcgaggcc tgattttgcg attcgatccc ggcgaccatg acatgcttcc cgcaatcccg
    15961 cgcttcatca atcaatcggc ttaacaattt acgccccaga cctttgccct gatgatcggg
    16021 atggacataa accgaatgtt ccacggtatg gcgaaaacca tcgaaactac gccagtcgcc
    16081 aaacgaggca tatcccgtca ctacgccatc ttcttcactt accagcaccg gataacctgc
    16141 tatggtccgc gcttcaaacc aggcaatgcg gttatctgca tcaaccgttt ggtcattcca
    16201 gatagccgcc gtatacaaca cggcgtggtt ataaatttcc gcaatggcag cacaatcggc
    16261 tttgcgggca aaacggatgg acatgtctgg cctcgataaa ataatgatga tgatgttatc
    16321 aatactatcg aaaacagccc gaagcaacgg attcctcgat gaaattattg cgtgttgcag
    16381 aggggatgtg acggctgcaa acaaaaccaa tcccgtgagc ctgctactat tgatggattc
    16441 atgccaaaag cacaggagag cattatgggg caacaaaagc agcgtaatcg acgttgggtt
    16501 ctggcctcgc gtccacatgg cgcaccggtt ccggagaatt tccgtcttga agaagatgat
    16561 gtcgccacac cgggtgaagg acaggtgtta ctgcgcacag tttatttgtc cctggacccg
    16621 tatatgcgtg gacgtatgag cgatgagcca tcttattcac cgcctgttga tattggcggc
    16681 gtgatggtcg gcggcacggt gagccgtgtt gtggagtcga atcatcctaa ttatcagcct
    16741 ggcgactggg tgctgggcta cagtggatgg caggactatg acatatccag tggtgatgat
    16801 ctggtgaaac ttggcgatca tccgcaaaat ccatcgtggt cgctgggtgt gctggggatg
    16861 ccaggcttta ccgcttatat ggggctgctg gatatcggtc agcctaaaga gggcgaaacg
    16921 ttggtggtag ctgcggcgac aggtcctgtg ggagcaacgg tggggcaaat cggcaaactt
    16981 aaaggttgca gggtggtggg ggttgccggt ggcgcggaaa aatgccgcca tgctatcgag
    17041 gtgctgggct tcgatgtttg tcttgatcac cacgcggatg attttgccga acaactggtg
    17101 aaagcgtgcc caaaaggtat tgatatctat tatgaaaacg tgggcggtaa ggtatttgat
    17161 gcagtgctac cattacttaa tacatctgcg cgcattcccg tttgtggctt agtgagcagc
    17221 tataacgcta cagagctacc acccggtccg gatcgtttac ccctgttgat ggcaacggtg
    17281 ctgaaaaaac gtattcgctt gcaagggttt attatcgctc aggattatgg tcaccgcatc
    17341 catgagtttc agaaggagat ggggcaatgg gtgaaagagg ataaaatcca ctaccacgaa
    17401 gatattactg atggtttaga gaatgcgcca cagacgttta tcggcttgct gaagggtaaa
    17461 aacttcggca aagtggtgat ccgcgtcgcg ggtgatgatt aaagtttcaa cggcggcgta
    17521 agccgccata aaaatccagg caaaaagctt attttcagct ttaattaact aaccacgtca
    17581 ttgttctgct gctaataaca acaaaggtgg gtggtttatg attattcact ttaatacacc
    17641 aggtgaactc cttctgccat gcaggcaggg ttggacagaa aacacgcgaa ggaattaaaa
    17701 tgcctggaac gggaaaaatg aaacatgtca gtttgactct gcaggttgag aacgacctga
    17761 aacatcagct tagtattggc gcattaaaac ctggcgcacg cctgattact aaaaatctgg
    17821 cggagcaatt aggtatgagt attacacctg tgcgtgaagc attattacgt ctggtttcgg
    17881 tgaatgcgct ttctgtcgca cctgcacaag catttacagt tccggaagtg gggaaacgtc
    17941 aattagacga aatcaatcgg atccgctacg aactggaatt aatggcagtt gctctggctg
    18001 ttgaaaacct caccccgcaa gaccttgcgg aactccagga attgctcgag aaattacagc
    18061 aggcgcaaga aaagggcgac atggaacaaa tcattaatgt aaacaggcta tttcgcttag
    18121 cgatttatca tcgctcaaat atgcccatcc tgtgtgagat gattgagcaa ctgtgggtca
    18181 ggatggggcc tggtttacat tatctctatg aagcgattaa tccagcggaa ttacgggagc
    18241 atatagaaaa ctatcatctg ttactcgctg cgttaaaagc aaaagacaaa gagggatgca
    18301 gacattgtct tgctgaaatt atgcaacaaa atattgctat tttataccag caatacaatc
    18361 gttaaagtaa atgcccggca aataccgggc atcaaggtaa ttactcaaat ctccacgcaa
    18421 tattcatgcc gacaccataa tttcgtccgg gcgaaggttc gtaatatcgc ccgtttgact
    18481 cattgacaat gacagaacca acgtattctt tatcgaataa attatcgaca cgaccaaata
    18541 aatccacagt taaattgtgg taattgtatt tatacccggt gaataagccg acgagagtat
    18601 aagacggcgc ttttgccgta ttttcatcat ctgccataat atcgcccata taacgcgctt
    18661 ccgtgcctgc ataccaacca tcttccggta cataacctat cgacgcaaag cccatattac
    18721 gggcgatccc tggcatccga ttaccgttac aatcctgttc attgcaaaca ttgctgcgat
    18781 aggtcgcatc aagccaggtc caggacgcgt ttacgcgaaa atctcctgcg aaacgttgat
    18841 cccatgccag ttcagcgcct tgacgacggg tctttccggc atttttgtaa gtcgtacgcc
    18901 caccgctact gctatcgaca acaatttcat catcagtgtc ggtctgaaac aatgcgagac
    18961 taagcagccc atcaccaata cgcgttttac tgccgatctc aattgtatcg ttggtggatg
    19021 gttttaaacc taagttcata ccgctttgcc catcagcacg ataagacagc tcattaatcg
    19081 tcggcgtttc aaaacctcgc ccggctgcca gatagatatt ccaggcatcg gtcattgcat
    19141 attttaacga accggcaggt agccatttat gataactggc atcaccgctg tcatcgccgt
    19201 tacccggagt aacgtaatgg tcgttggaat caaaccacac ggagctgtag cgcacgccag
    19261 catccagcga cagtttttcg ctcagctgcc actgcgtctg taaataggga tcgatgttcc
    19321 acatcagatt gcgttcgtcg cgacgcaact cacctttttg cccgtactcc ggcatgccgc
    19381 tattcaggcg gaagttattg tagcccttgc ggttttcact catgttttcg tagttcaggc
    19441 cggtagtgaa cgtgaccgga acgcccagtt caccacggtg tgtccagcgg ctgtctatcc
    19501 cctggtaatg gcgttgcagg gtaatcacgc cgcccgcatg tgacgggtta agttgggggg
    19561 ccatcgggat tgactggtac tgggtcgttt ctcgctctcc ggcatacatc atcacactca
    19621 tatcatcttg cgcgctcagg ctacgctcat agcgcaaccc agcctgagtt tgcttgatgg
    19681 tttttcgcgt gtcgtactgt tctgcacgag gcgcttgttg tggattcgcc ttccattctg
    19741 ctttggttag cccacctggg tcatctgctt tgatatccac actattgaaa atcaggctta
    19801 atttgctggc ttcatcaatg cgtacgccca gtttggcatt ggctaaattt ttctgtgcgc
    19861 cactatggtc acgatagccg tgggtcgtaa aacgcgtggt tgagacggtg taatcgacat
    19921 cgccgggctg tgtaccgtct cccgttgcgc ccgttgcttt cagcccatag cgccagctgc
    19981 caaaactgcc gtagtaacta ctggcttcaa tggttggtgg ctgttgtccg gtctgggtgg
    20041 tgacattcat tacaccgcca gacgcgttgc catacagggc agagaagggg ccacgcagca
    20101 cttccacatt ttgcacactg cttaaatcga tgttggatgt ttgcccttgc ccgtcgggca
    20161 tggtggcggg aataccgtcc acatacaggc gaataccgcg aattccgtaa gtggaacggg
    20221 agccaaatcc gcgaatcgac agctgtaaat cttgcgcata gttctgccgg ttttgtacct
    20281 gcaaaccagg cacgctggtc agtgattcgg acaagttaat gcgcggtgtt gccaggcgca
    20341 tctcctcgcc atccaccacg cttactgctg ctggcgtatc cagctcggaa accacctgcg
    20401 gtgcggcact gacaatcata gtctgttcat cagcggcaaa aacaacgggg gaaaggacaa
    20461 gcagtgcggg caaaacggtc tgtcggacgg aaaaaatctt catgaaaaaa gccaggttaa
    20521 gaatgggaaa acgctgtcat ggtaataaaa ttgtaaattt atggaaaacg aaacggcacg
    20581 atacgttaat taattgagaa aattgtagtc gtaacggcaa gaaatgctcc acatttgaga
    20641 aaataatgat taccattccc atttataaca agagcgtaac gatgattacg cttagcgaag
    20701 cattgtgaag cagcaaaaat attggttcat caaagggagt cgtcatgcat ttacgtcatc
    20761 tgttttcatc gcgcctgcgt ggttcattac tgttaggttc attgcttgtt gcttcatcat
    20821 tcagtacgca ggccgcagaa gaaatgctgc gtaaagcggt aggtaaaggt gcctacgaaa
    20881 tggcttatag ccagcaagaa aacgcgctgt ggctcgccac ttcgcaaagc cgcaaactgg
    20941 ataaaggtgg cgtggtttat cgtcttgatc cggtcactct ggaagtgacg caggcgatcc
    21001 ataacgatct caagccgttt ggtgccacca tcaataacac gactcagacg ttgtggtttg
    21061 gtaacaccgt aaacagcgcg gtcacggcga tagatgccaa aacgggcgaa gtgaaaggcc
    21121 gtctggtgct ggatgatcgt aagcgcacgg aagaggtgcg cccgctgcaa ccgcgtgagc
    21181 tggtagctga tgatgccacg aacaccgttt acatcagtgg tattggtaaa gagagcgtga
    21241 tttgggtcgt tgatggcgag aatatcaaac tgaaaaccgc catccagaac accggtaaaa
    21301 tgagtaccgg tctggcgctg gatagcaaag gcaaacgtct ttacaccact aacgctgacg
    21361 gcgaattgat taccatcgac accgccgaca ataaaatcct cagccgtaaa aagctgctgg
    21421 atgacggcaa agagcacttc tttatcaaca ttagccttga taccgccagg cagcgtgcat
    21481 ttatcaccga ttctaaagcg gcagaagtgt tagtggtcga tacccgtaat ggcaatattc
    21541 tggcgaaggt tgcggcaccg gaatcactgg ctgtgctgtt taacccagcg cgtaatgaag
    21601 cctacgtaac gcatcgtcag gcaggtaaag tcagtgtgat tgacgcgaaa agctataaag
    21661 tggtgaaaac gttcgatacg ccgactcatc cgaacagcct ggcgctgtct gccgatggca
    21721 aaacgctgta tgtcagtgta aaacaaaaat ccactaaaca gcaggaagct acccagccgg
    21781 acgatgtgat tcgtattgcg ctgtaataga tatggcgagg gtgaaaacgg agagacttgc
    21841 ctgactttta tcacgccgca tgcggcacac cttcgtcagt aatttgaaat tgcccggcac
    21901 gtagccgggc aattttgcaa ttagctcgcc gtttcaggct taaacacgat ttcctgtttt
    21961 tcttcatctt cctcgacgac tggcgcagtg ctgtgaattt cagcaacgcg tttgcgcaca
    22021 ccaaaccagc ctataaccag cagaataccg ataatcggca gcgccgcgat agtataagtc
    22081 ccgttcgggt aatcgaacgc catcagcaca aggacactca gtaaaaacag taatgtcagc
    22141 caggaagtga agggcgcgcc aggcagttta aaactgacat ccgctgcttt gccttcttta
    22201 atcgctttac gcaggcgcat ctggcacacg atgataaacg cccatgaagc gatgattccc
    22261 agcgacgcga agttcaacac aatctcaaat acgcgcgacg gcaccagata gttgaggaat
    22321 acgccgacga catacacaac tagtgttgct agaatcccgg catacggcac atgctgacga
    22381 ctcattttcg ccataaaact cggtgcggaa ccgcccatcg ccattgagcg cagaatacgt
    22441 ccggtgcagt acagacctga gttcaggctg gagagggcag cggtcagcac cacaatgttc
    22501 ataatgctgc cgatatatgg cacacccagt ttagagaaaa acgtcacgaa cggactttgc
    22561 cccgcctgat acgcgctcca cggcaatagc ataaccagca acaccacgga gccgacgtaa
    22621 aacaggccaa tacgccaaat cacgctgtta atcgctttag gcaccatggt ctgcggatct
    22681 ttacattctc cggcagctgt acccaccatt tcaatggagg caaaagcaaa cactacgccc
    22741 tgaatcaaca ccagcgcagg cagcaaaccg tgggggaaga agccgccatt atcggtgatt
    22801 aaatgaaagc cagtggtgtt gccatccagc ggctgaccac taccgaggaa cactgtaccc
    22861 acgaccaaaa aggtcacaat ggcgagcact ttaataagcg caaaccagaa ctccatctcc
    22921 gcaaaccatt tcacaccgat catattcatg gtgccaacga tggtaagtgc agcgagcgca
    22981 aagacccact gcggcacgcc gccaaacgca ccccagtaat gcatatacag agcgacggcg
    23041 gtaatatcaa caatccccgt catcgcccag ttgatgaagt acatccagcc agcaacataa
    23101 gcggctttct cacccaaaaa ctcacgggca taagagacaa aactgccact ggaagggcgg
    23161 tgtagcacca gctcacccaa tgcacgcaga ataaaaaacg aaaacaagcc acaaattaaa
    23221 taaaccagtg ccagtgctgg acccgccatt tgcagtcggg ctcctgcacc taaaaacaag
    23281 ccggtgccaa tcgcgccgcc aatggcgatc atctgtacct ggcgattgcc catcgctttg
    23341 tgatacccct cttcgtgggc attaagccag cggcgtttcg cggcgtgttg atctgaagtg
    23401 tcggtgtcgt gtttactcat tgctctccct gattgcttta atgaaaaagt catataagtt
    23461 gccatgaaca atgtttattc atcctggcga tgatagttat caccattatt ctttatgggt
    23521 ataaaactat cggcgcagca tcctacccgt tatcgataaa cgatgcaaaa catcccctta
    23581 caatcctgaa ggggattaat acaactgacg aaaaaatgac aaatcctttt gctggttaac
    23641 ctgtgtactg tcctacactt aatctttaaa agattgtgag gggcatatga ttaaggtgta
    23701 tggcgtaccc ggatggggct ctacaatcag tgagctgatg ctaacactgg ctgatattcc
    23761 ttatcagttc gttgatgtca gtggttttga ccacgaggga acttcacgcg agttattgaa
    23821 aaccctgaac ccgttgtgtc agattccgac actggcgctg gaaaatgacg agattatgac
    23881 ggagacagcg gcgattgcat tgatggttct cgatcgtcgc ccggaccttg cgccaccggt
    23941 tgggcgcgcc gaacgtcagc agtttcaacg gctattggtc tggctggttg ctaatgtcta
    24001 tccaacattc actttcgccg attaccctga gcgttgggct cctgacgcac ctgaacagtt
    24061 aaagaagaat gttattgaat atcgtaaatc gctttatatc tggctgaatt cacaattgac
    24121 cgctgaacct tatgcgtttg gtgagcaact aacgctggtg gactgttatc tttgcactat
    24181 gcgcacatgg gggcctgggc atgaatggtt tcaggataat gccacgaata tcagtgcgat
    24241 tgctgatgct gtatgtcagt taccgaaatt acaagaagta ttaaaaagga atgaaattat
    24301 ttgagtggga taccagagga ttatgttatt tattattca
//
LOCUS       DBANUU010000071        24334 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000071 DBANUU010000000
VERSION     DBANUU010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.071"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            85..768
                     /locus_tag="ACIE68_002739"
     CDS             85..768
                     /locus_tag="ACIE68_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFX5909028.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            812..5662
                     /gene="ehaG"
                     /locus_tag="ACIE68_002740"
     CDS             812..5662
                     /gene="ehaG"
                     /locus_tag="ACIE68_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFX5909029.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPSKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6030..7685
                     /gene="lldP"
                     /locus_tag="ACIE68_002741"
     CDS             6030..7685
                     /gene="lldP"
                     /locus_tag="ACIE68_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFX5909030.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7685..8461
                     /gene="lldR"
                     /locus_tag="ACIE68_002742"
     CDS             7685..8461
                     /gene="lldR"
                     /locus_tag="ACIE68_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFX5909031.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8458..9648
                     /gene="lldD"
                     /locus_tag="ACIE68_002743"
     CDS             8458..9648
                     /gene="lldD"
                     /locus_tag="ACIE68_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFX5909032.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9834..10307
                     /gene="trmL"
                     /locus_tag="ACIE68_002744"
     CDS             9834..10307
                     /gene="trmL"
                     /locus_tag="ACIE68_002744"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFX5909033.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10360..11181)
                     /gene="cysE"
                     /locus_tag="ACIE68_002745"
     CDS             complement(10360..11181)
                     /gene="cysE"
                     /locus_tag="ACIE68_002745"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFX5909034.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11261..12280)
                     /gene="gpsA"
                     /locus_tag="ACIE68_002746"
     CDS             complement(11261..12280)
                     /gene="gpsA"
                     /locus_tag="ACIE68_002746"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFX5909035.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12280..12747)
                     /gene="secB"
                     /locus_tag="ACIE68_002747"
     CDS             complement(12280..12747)
                     /gene="secB"
                     /locus_tag="ACIE68_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFX5909036.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12810..13061)
                     /gene="grxC"
                     /locus_tag="ACIE68_002748"
     CDS             complement(12810..13061)
                     /gene="grxC"
                     /locus_tag="ACIE68_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFX5909037.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13203..13634)
                     /locus_tag="ACIE68_002749"
     CDS             complement(13203..13634)
                     /locus_tag="ACIE68_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFX5909038.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13879..15423
                     /gene="gpmM"
                     /locus_tag="ACIE68_002750"
                     /gene_synonym="pgmI"
     CDS             13879..15423
                     /gene="gpmM"
                     /locus_tag="ACIE68_002750"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFX5909039.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15457..16716
                     /gene="envC"
                     /locus_tag="ACIE68_002751"
     CDS             15457..16716
                     /gene="envC"
                     /locus_tag="ACIE68_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFX5909040.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16720..17679
                     /locus_tag="ACIE68_002752"
     CDS             16720..17679
                     /locus_tag="ACIE68_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFX5909041.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17666..18700)
                     /gene="waaH"
                     /locus_tag="ACIE68_002753"
     CDS             complement(17666..18700)
                     /gene="waaH"
                     /locus_tag="ACIE68_002753"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFX5909042.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(18939..19964)
                     /gene="tdh"
                     /locus_tag="ACIE68_002754"
     CDS             complement(18939..19964)
                     /gene="tdh"
                     /locus_tag="ACIE68_002754"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFX5909043.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(19974..21170)
                     /gene="kbl"
                     /locus_tag="ACIE68_002755"
     CDS             complement(19974..21170)
                     /gene="kbl"
                     /locus_tag="ACIE68_002755"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFX5909044.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            21384..22316
                     /gene="rfaD"
                     /locus_tag="ACIE68_002756"
     CDS             21384..22316
                     /gene="rfaD"
                     /locus_tag="ACIE68_002756"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFX5909045.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNKGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVMEYMAWLNRDA"
     gene            22326..23372
                     /gene="rfaF"
                     /locus_tag="ACIE68_002757"
     CDS             22326..23372
                     /gene="rfaF"
                     /locus_tag="ACIE68_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFX5909046.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALFGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23376..>24334
                     /gene="rfaC"
                     /locus_tag="ACIE68_002758"
     CDS             23376..>24334
                     /gene="rfaC"
                     /locus_tag="ACIE68_002758"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFX5909047.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENA"
BASE COUNT         6107 a         6310 c         6313 g         5604 t
ORIGIN      
        1 atgtaaagca acatagatca ccaataatac aatggacaac tctaccggca ttgatcgctg
       61 gtcggaggaa ataaggattt ttacatgaac ttgaagaaga tttttttcag tgctgtcact
      121 gtttcagttc tctgcgcatt aacgggatgt gactacattg aagaaggcaa gccagaaagt
      181 agtttattga aacaacaaga agaacataac aataaaattg atctacttga aaaacaacaa
      241 gcacagctaa agagtcaact ggaaactatt caaaaacaac aaactggcat aataagcagt
      301 acaaagactt taactcacgt aattaaatcc gttaaagatc aacaaaacac ttttattttt
      361 accgagttta acccagcaaa aacgaagtat ttcatcctga acaatggttc ggtggcttta
      421 gcgggtcgag tgttatccat tgacgccaca gagaatggca gtgttattca tatttcactg
      481 gtcaacttat taagtacacc tatctcaaat attggtttta atgcgacatg gggtggtgaa
      541 aaacctgtcg atgccaaaga gtttgcccgg tggcaacaat tacttttcaa cacatcaatg
      601 aaatccacat tgaaattatt accaggtcaa tggcaagaca ttaatttgac cctgaagggt
      661 gtatcgccca ataacctggg atatctgaag ttagccatca acatggaaaa tattcagttc
      721 gacaatcttc cctctgctga aaatcggcag aaaagaagca aaaaataaat gcagttagta
      781 cgcttataac aaggaatagc gagcatcatt aatgaacaaa atatttaaag ttatctggaa
      841 ccctgcgaca gggaattata ctgttaccag cgaaacggca aaaagccgtg gcaagaaatc
      901 tgggcgcagt aagctgttaa tttctgcgct ggttgcgggt ggaatgttgt cgtcgtttgg
      961 ggcattggcg aatgccggga atgacaacgg tcagggtgtt gattacggta gtggatcagc
     1021 tggcgacggc tgggttgcta taggcaaagg ggcgaaagca aatactttta tgaacaccag
     1081 tggttccagt actgctgtgg gttatgacgc tatagctgaa ggccaatata gctctgccat
     1141 cgggtcaaaa acccatgcga ttggcggtgc atcaatggcc tttggggtta gtgcaatatc
     1201 agaaggcgat agaagtatag cactgggtgc ctcttcgtat tcattgggcc aatactcaat
     1261 ggccctcggc cgttattcaa aagcattggg taaattgtct attgctatgg gggactcttc
     1321 caaagcggaa ggagcaaacg ccattgccct gggaaatgcc actaaagcta ctgagattat
     1381 gagtattgct cttggcgaca ccgccaatgc gtcaaaagcg tattcaatgg cgctgggagc
     1441 aagtagcgtc gcatctgaag aaaacgcaat tgccctgggg cgtagcagtg tagctagcgg
     1501 tactgacagc ctcgcatttg gcagacaatc acttgccagc gcagcgaacg ctattgcgat
     1561 aggtgctgag accgaagccg ctgaaaatgc aactgctatt ggcaataatg cgaaggcaaa
     1621 agggactaat agcatggcaa tggggttcgg aagccttgcc gataaagtca atactatcgc
     1681 attaggaaat ggcagccagg ctctggcaga taatgcaatc gccataggcc agggcaacaa
     1741 agctgatggc gtggatgcca tcgctctggg taatggtagc cagtcgagag gcttaaacac
     1801 cattgcctta ggcacagcca gtaatgcaac tggtgataag agtcttgcgc ttggtagtaa
     1861 tagcagtgcc aacggtatta actctgtcgc actgggcgca gattccattg cggatttaga
     1921 caataccgtc tctgtcggca atagttcatt aaaacgcaag atcgttaatg tgaaaaatgg
     1981 cgcgatcaag tctgacagtt acgatgccat taatggttca cagctttatg ccattagcga
     2041 ctcggtagca aaaaggcttg gaggaggggc tgcagtagat gttgatgacg gtactgttac
     2101 agcaccaacc tacaatttaa aaaatggtag caaaaataac gtaggggctg cgctcgctgt
     2161 acttgatgaa aacaccctgc aatgggacca aaccaaaggc aaatacagcg ctgctcatgg
     2221 tactagtagc ccaactgcca gcgtaatcac cgatgttgcg gatggcacga tttcagcctc
     2281 cagtaaggat gcggttaacg gttcccaact gaaagctacc aatgacgatg tcgaagccaa
     2341 caccgccaat atcgctacta ataccagcaa cattgccacg aatacggcaa atattgccac
     2401 caataccacc aatatcacca acctgacgga ttccgttggt gaccttcagg ctgatgccct
     2461 gctctggaac gaaactaaaa aggcattcag tgcagctcac ggccaggata ccaccagcaa
     2521 aatcaccaac gttaaagatg ccgacctgac ggctgacagc actgatgctg ttaacggctc
     2581 tcagctgaaa accaccaacg atgctgtggc gacgaatacc accaatatcg ccaataacac
     2641 ttccaatatt gccactaaca ccaccaacat ctctaacctg actgagacgg tgactaatct
     2701 tggtgaggat gcgctgaaat gggataagga caatggtgta ttcactgcag ctcatggcac
     2761 cgagaccacc agcaaaatca ccaacgttaa agatggcgac ctgacgactg gcagcaccga
     2821 tgccgttaac ggctctcagc tgaaaaccac caacgatgcc gtggcgacga ataccaccaa
     2881 tatcgccact aacaccacca acatctctaa tctgactgag acggtgacta atcttggtga
     2941 ggatgcgctg aaatgggata aggacaatgg tgtcttcact gcagctcatg gcaacaatac
     3001 cgccagcaaa atcaccaata tcctggacgg cacagtcact gcaaccagtt ccgatgccat
     3061 taacggtagc cagctttatg acttaagcag caatatcgcc acctacttcg gcggcaatgc
     3121 ttctgtgaat actgacggtg tgtttaccgg tccaacctac aaaatcggtg aaacaaatta
     3181 ttataacgtc ggcgatgcac tggctgcgat taactcctca tttagcacgt ctctcggcga
     3241 tgctctgctt tgggatgcca ccgcaggtaa attcagtgcc aaacacggta ctaatggtga
     3301 cgcaagcgtg atcactgatg tcgcagatgg tgaaatttca gactccagtt ctgacgcagt
     3361 aaacggctca caactccacg gcgtgagcag ttatgttgtt gatgcgctgg ggggtggtgc
     3421 cgaagtcaat gcagacggca ccatcactgc gccgacgtac accattgcta atgctgatta
     3481 cgataatgtc ggtgatgccc tgaatgctat cgataccact cttgacgacg ctctgctctg
     3541 ggatgcggac gccggtgaaa atggtgcatt tagcgccgct cacggaaaag ataaaactgc
     3601 cagtgtaatc actaacgtcg ctaacggtgc aatctctgct gccagcagcg acgcgattaa
     3661 cggctcacaa ctctatacca ccaataagta catcgctgat gcgctgggtg gtgacgcaga
     3721 agtcaacgcc gacggcacca tcaccgcacc gacttacacc attgcgaacg ccgagtacaa
     3781 caacgtcggt gacgccctgg atgcgcttga tgataacgcc ctgctgtggg atgagactgc
     3841 caatggcggt gctggagcct acaatgccag ccatgacggt aaagccagca tcatcactaa
     3901 tgtcgctaat ggcagtatta gtgaggacag taccgatgca gtgaacggtt ctcagttgaa
     3961 tgcgacgaat atgatgattg agcagaacac ccaaattatc aatcagctcg ctggtaacac
     4021 cgacgcaacc tatatccaag aaaacggtgc gggtattaac tatgtgcgta ctaacgacga
     4081 cggcttagcg ttcaacgacg ccagcgcaca gggtgttggc gctacagcta taggttataa
     4141 ctctgtcgcc aaaggcgata gcagcgtagc tattggtcag ggcagctaca gcgacgttga
     4201 tacgggtatc gccctgggta gcagctctgt ttccagccga gtgattgcca aaggctcccg
     4261 tgacaccagc ataacggaaa atggcgttgt tattggttac gacaccacgg atggcgaact
     4321 gctcggtgca ttgtctatcg gtgatgacgg taaatatcgt caaatcatca acgtagccga
     4381 tggttccgaa gcccatgacg ccgttacggt tcgtcaattg cagaatgcga ttggtgctgt
     4441 agcaacgaca ccgtccaaat acttccatgc taattcaacg gaagaagatt cactggcagt
     4501 gggaactgac tcgctggcaa tgggtgcgaa aaccatcgtg aatggcgata aaggtattgg
     4561 tatcggttat ggtgcctacg tggacgcgaa tgcacttaac ggcattgcca ttggtagcaa
     4621 tgcgcaagtc attcatgtca acagtattgc gataggtaat ggttctacga ccactcgtgg
     4681 cgctcaaacc aattataccg cctacaacat ggacgcaccg cagaactctg tcggtgaatt
     4741 ctcagttggt agtgcggatg gtcaacgtca gatcactaac gtcgcagcag gttcggctga
     4801 taccgatgcg gtcaacgtgg gtcagttgaa agtaacggat gcgcaggttt cccagaatac
     4861 ccagagcatt actaacctgg ataatcgggt aacgaatctt gattcacgcg tcaccaatat
     4921 cgaaaacggt attggcgata tcgtcaccac cggtagcacc aagtacttca agaccaatac
     4981 cgatggtgta gatgccagcg cgcagggtaa agatagcgtt gctattggtt ctggttccat
     5041 tgctgccgct gacaacagcg tcgcactggg taccggttcc gttgcagagg aagaaaatac
     5101 aatctctgta ggttcttcta ctaaccaacg ccgtatcacc aacgtagctg ccggtgttaa
     5161 tgccaccgat gcggttaacg tgtcgcagct gaaatcttct gaagcaggcg gcgtgcgcta
     5221 cgacaccaaa gctgatggtt ctatcgacta tagcaatatc accctcggtg gcggcaacgg
     5281 cggtacgact cgtatcagca acgtctccgc tggcgtcaac aacaacgacg cggtgaatta
     5341 cgcgcagttg aagcaaagcg tgcaggaaac gaagcaatac accgatcagc gaatggttga
     5401 gatggataac aaactgtcta aaactgaaag caagttgagc ggtggtatcg cttctgcaat
     5461 ggcaatgacc ggtctgccgc aggcttacac tccaggtgcc agcatggcct ctattggtgg
     5521 cggtacttac aacggtgaat cggcagttgc tttaggtgta tcgatggtga gcgccaatgg
     5581 tcgttgggtc tacaaattac aaggtagtac caatagccag ggtgaatact ccgccgcact
     5641 cggtgccggt attcagtggt aatgatccat taagttagtg tgactaaggg caaacgccaa
     5701 attgcctgat gcgttacgcg catcaggcaa gataaggcgc tccgggtcaa caatctaagg
     5761 gcaattctct gatgaggatt gcccttttct ttaccagaca tctcccccca caagaattgg
     5821 ccctaccaat tcttcgctta tctgacctct ggttcacaat tccccaatta aaactcacat
     5881 caatattgcc aatacataac atttagttaa ccattcattg tcattatccc tacacaacac
     5941 aattggcagt gccactttta cacaacgtgt gacaaggaga tgagcaacag actcattaca
     6001 cgatgtgcgt ggactccagg agacctgcaa tgaatctctg gcaacaaaac tacgatcccg
     6061 ccgggaatat ctggctttcc agtctgatag catcgcttcc catcctgttt ttcttctttg
     6121 cgctgattaa gctcaaactg aaaggatacg tcgccgcctc atggacggta gcaatcgccc
     6181 ttgccgtggc tttgctgttc tataaaatgc cggtcgctaa cgcgctggcc tcggtggttt
     6241 atggcttctt ctatggtttg tggcccatcg cgtggatcat tattgcagca gtgttcgtct
     6301 ataagatctc ggtgaaaacc gggcagtttg acatcattcg ctcgtctatt ctttcgataa
     6361 cccctgacca gcgtctgcaa atgctgatcg tcggtttctg tttcggcgct ttccttgaag
     6421 gagccgcagg ctttggcgca ccggtagcaa ttaccgccgc attgctggtc ggcctgggtt
     6481 ttaaaccgct gtacgccgcc ggactgtgcc tgattgttaa caccgcgcca gtggcatttg
     6541 gcgcgatggg cattccaatc ctggttgccg gacaagtaac aggcatcgac agctttgaga
     6601 ttggccagat ggtggggcgg cagctaccgt ttatgaccat tatcgtgctg ttctggatca
     6661 tggcgattat ggacggctgg cgcggtatca aggagacgtg gcctgcggtc gtggttgcgg
     6721 gcggctcgtt tgccatcgct cagtacctta gctctaactt cattgggccg gagctgccgg
     6781 acattatctc ttcgctggta tcactgctct gcctgacgct gttcctcaaa cgctggcagc
     6841 cagtgcgcgt attccgtttt ggtgatttgg gggcgtcaca ggttgatatg acgctggccc
     6901 acaccggtta cactgcgggt caggtgttac gtgcctggac accgttcctg ttcctgacag
     6961 ctaccgtaac actgtggagt atcccgccgt ttaaagccct gttcgcatcg ggtggtgcgc
     7021 tgtatgagtg ggtgatcaac attccggtgc cgtacctcga taaactggtt gcccgtatgc
     7081 caccagtggt cagcgaggct acagcctatg ccgccgtgtt taagtttgac tggttctctg
     7141 ccaccggcac cgccattctg tttgctgcac tgctctcgat tgtctggctg aagatgaaac
     7201 cgtctgacgc tatcagcacc ttcggcagca cgctgaaaga actggctcta cccatctact
     7261 ccatcggcat ggtgctggcg ttcgccttta tctcgaacta ttccggactg tcatcaacac
     7321 tggcactggc actggcgcac accggtcatg cattcacctt cttctcgccg ttcctcggct
     7381 ggctgggggt attcctgacc gggtcggata cctcatctaa cgccctgttc gccgcgctgc
     7441 aagccaccgc agcacaacaa attggcgtct ctgatctgtt gctggttgcc gccaatacca
     7501 ccggtggcgt caccggtaag atgatctccc cgcaatctat cgctatcgcc tgtgcggcgg
     7561 taggcctggt gggcaaagag tctgatttgt tccgctttac tgtcaaacac agcctgatct
     7621 tcacctgtat ggtgggcgtg atcaccacgc ttcaggctta tgtcttaacg tggatgattc
     7681 cttaatgatt gttttaccca gacgcctgtc agacgaggtt gccgatcgtg tgcgggcgct
     7741 gattgatgaa aaaaacctgg aagcgggcat gaagttgccc gctgagcgcc aactggcgat
     7801 gcaactcggc gtatcacgta attcactgcg cgaggcgctg gcaaaactgg tgagcgaagg
     7861 cgtgctgctc agtcgacgcg gcggcgggac gtttattcgc tggcgtcatg acacatggtc
     7921 ggagcaaaac atcgtccagc cgctaaaaac gctgatggcc gatgatccgg attacagttt
     7981 cgatattctg gaagcccgct acgccattga agccagcacc gcatggcatg cggcaatgcg
     8041 cgccacacct ggcgacaaag aaaagattca gctttgcttt gaagcaacgc taagtgaaga
     8101 cccggatatc gcctcacaag cggacgttcg ttttcatctg gcgattgccg aagcctcaca
     8161 taacatcgtg ctgctgcaaa ccatgcgcgg tttcttcgat gtcctgcaat cctcagtgaa
     8221 gcatagccgt cagcggatgt atctggtgcc accggttttt tcacaactga ccgaacaaca
     8281 tcaggctgtc attgacgcca tttttgctgg tgatgccgac ggggcgcgta aagcaatgat
     8341 ggcgcacctt agttttgttc acaccaccat gaaacgattc gatgaagatc aggctcgcca
     8401 cgcacggatt acccgcctgc ccggtgagca taatgagcat tcgagggaga aaaacgcatg
     8461 attatttccg cagccagcga ttatcgcgcc gcagcgcaac gcattctgcc gccgttcctg
     8521 ttccactata tggatggggg tgcatattct gaatacacgc tgcgccgcaa cgtggaagat
     8581 ttgtcagaag tggcgctgcg ccagcgtatt ctgaaaaaca tgtccgactt aagcctggaa
     8641 acgacgctgt ttaatgagaa attgtcgatg ccggtggcgc taggtccggt aggtttgtgt
     8701 ggcatgtatg cgcgacgcgg cgaagttcag gcagccaaag cggcggatgc gcatggcatt
     8761 ccgtttactc tctcgacggt ttccgtttgc ccgattgaag aagtcgcgcc agccatcaag
     8821 cgcccaatgt ggttccagct ttatgtactg cgcgatcgcg gctttatgcg taacgcgctg
     8881 gagcgagcaa aagcagcggg ttgttcgacg ctggttttca ccgtggatat gccgacaccg
     8941 ggagcgcgtt atcgtgatgc gcattctgga atgagcggcc cgaacgcggc aatgcgccgc
     9001 tatttgcagg cggtgacgca tccgcaatgg gcgtgggatg tgggcctgaa cggtcgtccg
     9061 catgatttag gaaatatttc ggcttacctc ggcaaaccga ccggactgga agattacatc
     9121 ggctggctgg ggaataactt cgatccgtcc atctcatgga aagaccttga gtggatccgc
     9181 gatttctggg atggcccgat ggtgatcaaa gggatcctcg atccggaaga tgcgcgcgat
     9241 gcagtacgtt ttggtgctga tgggattgtg gtttctaacc acggtggccg ccagctggac
     9301 ggtgtgctct cttctgcccg tgcactgcct gctattgccg atgcggtgaa aggtgatatc
     9361 gccattctgg cggatagcgg aattcgtaac gggcttgatg tcgtgcgtat gattgcgctc
     9421 ggtgccgaca ccgtactgct gggtcgtgct ttcctgtatg cgctggcaac agcgggccag
     9481 gcgggtgtag ctaacctgct aaatctgatc gagaaagaga tgaaagtggc aatgacgctg
     9541 actggcgcga aatcgatcag tgaaattacg caagattcgc tggtgcaggg gctgggtaaa
     9601 gagttgccta cggcactggc tccgatggcg aaagggaatg cggcatagtc gtttgagccc
     9661 cctcacccta accctctccc tcagggagtg gggaccgttc ggcgctgtat gtactccctc
     9721 actctgaaac gacaccgcac tctttttttc tccctcgccc ctccggggtg aggggaaaag
     9781 gccgcactgc tcaccaattt tctgctatcc tgcccccgca ctaagggggc agtatgctaa
     9841 acatcgtact ttacgaacca gaaattccgc caaatactgg caacatcatc cgtctttgcg
     9901 ctaataccgg ctttcgtctg catatcatcg aaccgatggg atttgcctgg gacgataagc
     9961 gcctgcgccg cgcggggctg gactatcacg agtttaccgc cgttacgcgt catcatgact
    10021 atcgcgcgtt cctcgaagca gaaaatcccc agcgcctgtt tgccctcacc acgaaaggta
    10081 cgcctgctca cagcgccgta agctatcagg atggcgatta tctgatgttc ggcccggaaa
    10141 cacgcggcct gccagcgagc attcttgatg ccctgcccgc tgaacaaaaa attcgcattc
    10201 cgatggtgcc ggacagccgc agcatgaatc tgtccaatgc ggtgtcggta gtggtgtatg
    10261 aagcctggcg gcagctggga tatccgggag cgttgttgag agattagtta ctgtatgccg
    10321 gatagatgat tacatcgcat ccggcacgat cacaggacat tagatcccat ccccatactc
    10381 aaatgtatgg ttaataccgt tgaaatgctg gtccatatcc attgatggct tatcgctgtc
    10441 tggtttaccg acaatacgag ccggaacgcc agcggcggtt gtatgcggcg gcaccggttg
    10501 cagcaccacg gaacctgcgc caatcttcgc gccgcgacca acttcaatat tgccgaggat
    10561 tttcgcgccc gcgccaatca tcacaccttc acgaattttc gggtgacggt caccaccaga
    10621 tttacccgta ccgccaagcg tcacagattg cagaatcgat acgtcgtttt caattaccgc
    10681 cgtttcaccc acaacgatgc ccgtggcgtg gtcgagcata atgccacggc caatttttgc
    10741 tgccgggtga atatcgacct ggaacgtcac agaaacctgg ttttgcagaa agattgccaa
    10801 tgcgcgacgc ccctgattcc acaaccagtg accgatgcga taggcctgca aggcatgaaa
    10861 acccttcagg tataacaacg gggttgagta tttatcgact gccgggtcgc gggtacgcac
    10921 cgcctgaata tcacaggccg cagaggcgat catttccggg tcagcggcgt aggcttcttc
    10981 caccacttca cggatagcaa tagcaggcat aattggcgat gacagcttgt tcgccagcat
    11041 gtagctcagt gcactaccaa ggttttcgtg cttgagtagc gtcgcgtggt aaaaactggc
    11101 cagcattggc tcacagtccg ccagcgttct ggcttcggct ttaatattgt tccagacaat
    11161 ttccagttct tcacacgaca ttgcttactc cacacgatga gataatgacc ggcccgttct
    11221 gcgcgggccg ggtcatagcg gtaacaaagg ttccctgggg ttagtggctg ctgcgctcgt
    11281 ccttgcgtgc acgacctaat aacgtcaatg ctgcctcgcg cgcgtttttt ccgcaatata
    11341 atacttgata aatttcctcg gttattggca tttcaacgcc gaagcgatgc gccagttcgc
    11401 ggacttcttt cgtattgcgg tagccttcca ccacctgacc aatcttctcc tgcgcgcttt
    11461 gtacatccat gccctgaccg agcatcatgc caaaacggcg gttacgcgac tggttgtcgg
    11521 tacaggtaag caccagatcg ccaagccccg ccatgcccat aaaggtggca ggatcggcac
    11581 ccagcgccgc accaagacgc gacatttcag ccagcccacg ggtgatcagc gccgtacgcg
    11641 cattcgcacc aaaaccgata ccgtcggaca tccccgcgcc aatggcaatg acgtttttca
    11701 ccgcaccgcc aagctgcaca ccgatgaaat ccgggttgct gtaaacgcgg aaacttttgc
    11761 cacagtgcag caattgttgg agatcatcgg caaaggtctg gtcggtagag gccaacgaaa
    11821 tagccgtcgg taaacctgcc gccagttctt tcgcaaacgt tgggccagag ataaccgcca
    11881 gcggaatttg atcgcctaag gcctcacgcg ccacgtcctg taacagacgt ccggtttccg
    11941 cttccagccc tttggtcgcc cacaccagac gcgcatcagg acgcatcagc ggtttaatct
    12001 ggcgcagcac ttcaccaaag acatggctgg gtacgacgac gagaatatta cggctggctg
    12061 ccagcgcagt ggcgagatcg ctttcaagat ggagcgtatc gggaaaaggc acatcgggga
    12121 gaaacgcggc gttacagcgg tcgcgttcaa gcgttgcgat atgttcaggg tcatggcccc
    12181 agaggacaac ctcgtggcca tttcttgcca gggtaatggc aagagcggtg ccgtacgagc
    12241 cggcaccgat cacagtcatt gaagcattac gttggttcat caggcatcct gatgttcttc
    12301 agtaccttcg ccagcctgct gctgcaaata gttcatgaac agcgcatcga agttaaccgg
    12361 cgcaaggttc agttgcggga atgtaccgcg ggataccatg ctggtgatgc actcacgagc
    12421 atacgggaac agaatgttcg ggcagtatgc gcccaggcaa tgtgccatct gggtgccttc
    12481 gatacccgcg atggagaaaa taccgccctg ctgaacttca cacaggaacg cggtttcttc
    12541 gcccagggag gccgttacgg taacacgcag taccacttcg taaacgtcat ctgccagttg
    12601 ggaagatgcc gtatccagat caagtttaac ttctggctgc caatctttct ggaaaacgtg
    12661 cggcgcgttc ggcgcttcga aagaaatatc cttggtataa atacgttgga tctggaaagt
    12721 catttcggtg ttgttttgtt ctgacatgtg tagaaaaccc ttaagtgttg tccttaaata
    12781 ctgcgcaatg taatgccaac gttcacacgt tatttcagca ggggatccag tccaccacgt
    12841 gcatccaatg catacaagtc atcacagccg ccaatgtgct gtgcgtcaat aaaaatctgg
    12901 ggaaccgtgg tgcgaccgct gcgtttgatc atctcttcac gcttggcggc attgccatcg
    12961 atcggcaact cctgaaaact cacgcccttg ctgctcagca gtgcttttgc acgatggcaa
    13021 tacgggcagg tttctttggt atagatttca acattggcca taatttagct cctgtttttt
    13081 taccctgaaa tttcatgttg cagggcggct gcaaaatgga gtgtccccag aagcttataa
    13141 caataagtcg ctgggggcct aattgcagct aacggcctgc atcatgaaag acgacaggta
    13201 aattatttgc cgcgcaccaa aggcaagttt tcgccagccc agccagcgac gccttctttc
    13261 agcacgaata cttgcgcaaa accagctttc gtcagtgcgt ttgcaggctc ctggcactgc
    13321 atgccagaac cgtctaccac gataaccggt ttgtctttgt gcttctcaag ctcaccaaca
    13381 ttgttggctt tgatttcgct cggcaacaga ttgatagaac ctgcgatatg gcctttacgg
    13441 aagtcatcac gctgacgtaa atccacaacc acagcgtctt ctttgttgat cagacgcgta
    13501 gcttcaccac gagtaatcac cttcactttc gaggtcaggc tcttaaacgt agtcacaaga
    13561 accgccacca gtaacgcgat ccaggcgata ctcagtatgg gatgacggcc aacaaattgc
    13621 ataatttctt gcatgggggg taacaactcc cgacgtagtg attaaaaaaa ccaggaaagg
    13681 agtatacctg cgcggtgcgg caaatacagc cagcgcgtta actggaatgc aattttgcgg
    13741 ggcacgacga aaaaaaagcg caaatctggc atcggcttgc cgcgagcggt cgtatatttt
    13801 gatcttcaga ggctatttta tcgattcagc tgtagtaaaa ttacgcaaat tttgactctt
    13861 gagtatgagg ttgtcgcaat gtcggtttct aaaaaaccta tggtactggt gattctggat
    13921 ggctatggct atcgtgaaga acagcaggat aacgccattt ttagtgctaa aaccccggta
    13981 atggatgcac tgtgggccaa tcgtccgcat accctgatcg acgcttccgg tctggaagtc
    14041 ggtctgcctg accgtcagat gggtaactcc gaagtaggcc acgttaacct gggtgccggc
    14101 cgcatcgtgt atcaggacct gactcgtctg gacgttgaaa tcaaagatcg cgctttcttt
    14161 gctaatccag tgctgactgg cgcggtagat aaagcgaaaa acgcaggtaa agcagtacac
    14221 attatgggtc tgctctccgc aggcggcgta cacagccacg aagatcacat catggcgatg
    14281 gtagaactgg cagctgaacg cggcgcagaa aaaatctacc tgcacgcatt ccttgacggt
    14341 cgcgacaccc cgccgcgcag tgctgaatcc tcgctgaaaa aatttgaaga gaaatttgct
    14401 gcgctgggta aaggccgcgt agcgtccatc attggtcgtt actacgcgat ggaccgcgat
    14461 aaccgttggg atcgcgttga aaaagcctat gacctgctga ctctggcgca gggtgagttc
    14521 caggccgata ctgccgttgc tggtttgcag gcagcttatg ctcgcgacga aaacgacgag
    14581 ttcgtgaaag cgaccgttat tcgtgcagaa ggtcagccag atgcggcaat ggaagacggc
    14641 gatgcgctga ttttcatgaa cttccgtgct gaccgcgcgc gcgaaatcac tcgtgcattc
    14701 gtaaacgcag atttcgacgg cttcgcgcgt aagaaagtgg ttaacgtcga tttcgtgatg
    14761 ctgaccgaat acgccgctga catcaaaact gcggttgctt acccacccgc ttccctggtt
    14821 aacaccttcg gcgagtggat ggcgaaaaac gacaaaactc agttgcgtat ttccgaaacc
    14881 gaaaaatatg cccacgttac tttcttcttc aacggtggcg tagaagagtc gttcaaaggc
    14941 gaagatcgca ttctgatcaa ctcaccgaaa gtggctacct acgatctgca accggaaatg
    15001 agctccgcag agctgaccga aaaactggtt gcggccatca agagcggcaa atacgacacc
    15061 attatctgta actatccgaa cggcgacatg gtaggtcaca ccggggtgat ggaagcggcg
    15121 gttaaagcgg ttgaagcact ggatcactgc gtggaagaag tcgcgaaagc ggttgagtcc
    15181 gtgggtggac aactgctgat caccgctgac cacggtaacg ctgaacagat gcgcgatccg
    15241 gcaacgggtc aggcacacac ggcacacacc aacctgccag ttccgctgat ttacgttggt
    15301 gataagaacg tgaaagcggt tgcaggcggc aaactttctg acatcgcgcc gaccatgttg
    15361 tcgctgatgg gtatggaaat cccgcaagag atgactggta agccgctgtt catcgtggaa
    15421 taatccctcc ccatgagggg aaaggcgatt aataccatga cacgggccgt gaaaccgcgc
    15481 aggtttgcaa tcaggcccat catctacgcc agcgttctta gcgctggcgt attgttgtgc
    15541 gccttttccg cccacgcgga tgagcgtgac caactcaaat ctattcaggc cgatatcgcc
    15601 gcgaaagagc gcgcggtacg ccaaaagcaa caacaacgcg caagcctgct cgcacaattg
    15661 aaaaagcagg aagaagcgat ctctgaagcc acccgtaagc tgcgcgaaac gcaaaacacg
    15721 ctcaatcaac tcaataaaca gattgatgag atgaacgcgt cgattgccaa actggagcag
    15781 caaaaagccg cccaggagcg cagcctcgcc gcacaactgg atgccgcatt ccgtcagggc
    15841 gagcataccg gtattcagct aattctcagc ggtgaagaaa gccagcgtgg acagcgttta
    15901 caggcttatt tcggctatct caaccaggcg cgacaagaaa ccattgccca gttgaagcaa
    15961 acgcgtgaag aagtcgccat gcagcgtgct gaactggaag agaaacagag cgagcaacaa
    16021 acgctgttat atgaacagcg cgcccaacag gcgaaactga ctcaggctct gaacgagcgt
    16081 aaaaagacgc tggcggggct ggaatcttcc atccagcaag gtcagcagca gctaagtgaa
    16141 ctgcgtgcca acgaatcccg tctgcgtaac agcattgccc gtgcggaagc cgcggcgaaa
    16201 gcgcgtgctg aacgagaagc gcgtgaggcc caggcggttc gcgaccgcca gaaagaagcg
    16261 acgcgcaaag gcaccaccta taaaccgacc gaaagcgaaa aatcgctgat gtcccgaact
    16321 ggtggcctgg gtgcgccgcg tggtcaggca ttctggccgg ttcgcgggcc gacgctgcat
    16381 cgctatggtg aacagctaca gggcgaacta cgctggaaag gaatggttat cggtgcctct
    16441 gaaggtactg aagttaaagc gattgccgac ggtcgggtga ttctggctga ctggctgcaa
    16501 ggttacggtc tggtggtggt ggttgagcac ggtaaaggcg acatgagtct ttacggctat
    16561 aatcagagcg cactggtgag cgttggttcg caggttcgcg cgggccagcc aattgcactg
    16621 gtgggcagca gtggcggtca gggtcggcct tcactctatt tcgaaattcg ccgccagggt
    16681 caggcggtca atccacagcc gtggttggga agataagttt tgtttccatt tcgtcgtaac
    16741 gttcttgcat ttgccgctct gttggcgctc tcctcccccg tacttgctgg caaacttgcc
    16801 atcgtcattg atgattttgg gtatcgcccg cacaacgaaa accaggtgct ggcgatgccc
    16861 tccgctatct ccgtcgctgt attacccgat tcaccgcacg ccagagagat ggcgaccaaa
    16921 gcgcataaca gcgggcacga agtgttgatt catctcccga tggcaccact gagtaaacag
    16981 ccgctggaga aaaatacgct gcgcccggag atgagcagcg acgaaattga gcgcattatt
    17041 cgtagtgcgg tcaataacgt gccctatgcc gtggggatca acaaccacat gggtagcaag
    17101 atgacctcta acctgtttgg tatgcagaaa gtgatgcagg cactggagcg ttacaatctt
    17161 tacttcctcg acagcgtaac catcggtaat acccaggcga tgcgcgccgc gcaaggtact
    17221 ggcgtgaagg tgatcaaacg gaaggtgttc ctcgacgatt cgcagaacga agcggacatc
    17281 cgtgtgcaat ttaatcgcgc aattgatctg gcgcgtcgca acggttcaac cattgccatt
    17341 gggcatcctc acccttcaac ggtacgcgtg ctacaacaga tggtttataa cctgccgcca
    17401 gacattacgc tggtgaaagc cagcagcttg ctgaatgaac cgcaggttga tacttctaca
    17461 ccgccgaaaa acgctgtgcc tgatgcaccg cgtaatccgt tccgtggcgt gaagctgtgc
    17521 aaaccgaaga aaccgataga acctgtttat gctaatcgct tctttgaagt attaagcgaa
    17581 agcatcagcc agagcacgct gatcgtttac ttccagcatc agtggcaagg ctggggcaaa
    17641 cagcccgaag cggcgaagtt taacgctagc gcaaattaaa ggcgctacgc gtaatgcgat
    17701 ggtgcgaccg cgttttgtcg cgccattgcc ataagcggaa agaccacagt aacgcctggt
    17761 aaccgacctt cacgctgcgc acattggtaa tcaggcgctt atacataccg gaagtaaata
    17821 tctcggcaat catccgttgg cgggtaagaa tatccggctc tttgcgcacc gcatggcaaa
    17881 cacgcaatgc ttcgtaagtt atttgctgat gaaattccgg ataaatcgta attttgtcgg
    17941 cataatttcg atttaatttc tccagcaggc gggtaatctt aatatagtga cgttgataat
    18001 taaggttttt attcccttgt ctatgcaacc gactcactga cgtattatgc agataatatt
    18061 tatataatga ttgctcggta tatcgcgcac gcagcgcgtt aaacatgaat tctgttgtcc
    18121 agacaatatc ctgatgatgt aatccggcaa taaatttaat gttattttta acaataacat
    18181 cacggcgata aacccccatc cagacaacgt gagtccaacg gcgcgaagaa agccccatcc
    18241 gcagccagtc cgggccggtt aatacgccgg ttgagcgaag gcgatcgctg gggatggatt
    18301 gccaggtttc tcccgtttca cgaaaacacc agtcagcgtt gcactgcgcc acgtcgaggt
    18361 catcctctaa cgccatggtc atcagcgttt cgtacatggt gggatagacc tcatcgtcag
    18421 catcgacaaa agcgacatat ttgcccgtcg ccacctcgat cccacgatta cgcgctaccg
    18481 atgcgccagc attcgcctga tgcaacaaac gaacgtgcgg atagttttct gcgtaatgct
    18541 ttgctatttc aacagaatta tccgttgaac catcgttaat aataatgatt tccagagcag
    18601 tccaggtttg cgtaattaaa gattccatac aagtgcggaa atcatcgccc gcattatata
    18661 acggaataat aacactaagt ttattggtgc tgttcatcat aaattaccga cgacctctga
    18721 agttatcctg acgttttaca ggctttgtat taagaaacta ttaagcctgt gagatattat
    18781 ttttacaggt gagagagtca cttcagggta gagagataat gagagcagct atataagaga
    18841 tcagcgcaat tacgatatct ttattacact gacaggattt tacatattta acggattatc
    18901 ctcaggcaaa agggctggaa taccagccct tgttcgtgtt aatcccaact cagaattact
    18961 ttcccggact ggcccgaacg catagcgtca aagcccttct ggaaatcatc gatagagaaa
    19021 cgatgggtaa tgatcggcga aagatcgagg ccagactgaa tcagcgccgc catcttgtac
    19081 caggtttcaa acatctcacg accgtaaata cctttaatga acaagccttt aaagatcact
    19141 ttggtccagt cgatagacat atcagacggc ggaataccca gcatcgcaat acggccgccg
    19201 tggttcatgg tatcaagcat ggtacgaaac gctggcggcg caccggacat ttccagaccg
    19261 acatcaaaac cttcggtcat gcctaactcc gccatcacgt cattgagatt ttctttggcg
    19321 acgttaaccg cacgggtgat acccatttta cgcgccagct caaggcggta ttcgttaaca
    19381 tcagtgatca ccacattgcg tgcaccaacg tgtttcgcca ccgccgctgc cataatacca
    19441 atcgggcctg caccagaaac cagcacatcc tcgccaacca gatcgaacga cagcgcggta
    19501 tgcacggcat taccgaaggg atcaaaaatg gaagccaggt cgtcggagat attgtcggga
    19561 attttgaagg cgttgaacgc cggaatcacc agatattcgg cgaagcagcc cgggcggtta
    19621 acgccgacgc cgatcgtgtt gcggcacaga tgggtacgac cgccacgaca gttgcgacag
    19681 tgaccgcagg tgatatggcc ttcgccagaa acgcgatcgc caatcttgaa gcccttcact
    19741 tcctgaccaa tacctaccac ttcaccgaca tattcatggc ccacgaccat cggcaccggg
    19801 atggtttttt gcgaccactc atcccagtta tagatgtgaa cgtcagtccc gcagatggct
    19861 gttttacgga ttttaatcag cagatcgtta tgcccgagtt ccggtacagg aacgtcggtc
    19921 atccagatgc cctcttccgc tttcagtttg gataacgctt tcatctcaca tcctcaggcg
    19981 ataacgccca gttgtttacc aatacgcgtg aatgcttcta ctgcacgcgt aatttgctca
    20041 ggggtatgcg ccgcagacat ctgggtacga atacgcgcct gacctttcgg aacgaccgga
    20101 tagaagaaac cggtgacgta aatgccctct ttttgcagct cacgggcaaa tttctgcgcc
    20161 actaccgcat caccaagcat gaccggaata atggcgtgat cggctcccgc cagggtaaag
    20221 cccgccgccg acatttgctc acggaactga cgcgcgttcg cccacagacg gtcacgcaac
    20281 tcgctacccg cttcaaccat ctccagcact ttgatggaag cggcgacaat cgctggtgcc
    20341 agcgagttgg agaacaggta cggacgagaa cgctggcgca gccactcaac cacttctttg
    20401 cgcgccgcgg tataaccacc agaagccccg cccagcgctt taccgagcgt gccggtgatg
    20461 atgtcgacac gccccatcac atcgcagtat tcatgggaac cacgaccatt ttcaccgaca
    20521 aaaccgaccg cgtgggagtc gtctaccatc accagggcat cgtatttatc tgccagatcg
    20581 caaacgccct tcagattggc aatcacgccg tccatcgaga acacaccatc ggtggcaatc
    20641 agcacatgac gcgcaccggc ttcacgcgct tctttcagac gagcttcaag ctcctgcata
    20701 tcgttgttgg catagcgata gcgtttagct ttacacagac gcacaccatc aataatagac
    20761 gcgtggttca gtgcgtcgga gataatggcg tcttccgcgc ccagcagcgt ttcaaacagg
    20821 ccaccgttag catcaaagca ggaagagtag agaatcgcat cttccatccc caggaatgcc
    20881 gccagttttt gttcaagctc tttatggctg tcctgagtgc cgcaaataaa acgcaccgaa
    20941 gccatgccga aaccgtgaga atccattccc gcctttgctg ccgcaatcag atccggatga
    21001 ttcgccagcc cgagatagtt gttggcacaa aagttaatga cgtggcttcc atcagccacg
    21061 gtgatatctg cttgctgcgc agacgtaata atgcgctctt ctttaaacaa cccttccgcc
    21121 cgtgcggttt ccagatcgtt ggttaactgc tgataaaatt ctccacgcat tgcgattctc
    21181 cagacagggc aaattccagc acatattacc caaacttata ggtcgcgacg agataacgcg
    21241 ttaacacttc tgcaaaattc aggataaata acgtccatca atctcacttt tggcgaatgg
    21301 ctgaagccaa cgccgtgtag tgatatgata gaagtattcg tgtctgagat tgtctctgac
    21361 tccataattc gaaggttaca gttatgatca tcgttaccgg cggcgcgggc tttatcggca
    21421 gcaacatcgt taaggccctg aatgataaag gcatcaccga tattctggtg gtggacaacc
    21481 tgaaagacgg caccaagttt gtgaacctgg tggatctgga tatcgcggac tatatggata
    21541 aggaagactt cctgatccag attatggctg gcgaagagtt cggcgatgtc gaagcgattt
    21601 tccacgaagg cgcatgctct tccaccaccg agtgggacgg caagtatatg atggataaca
    21661 actatcaata ctccaaggag ctgctgcact actgcctgga gcgcgaaatc ccgttcctgt
    21721 acgcttcttc cgcagccacc tacggcggac gcacctccga ctttattgaa tcccgcgagt
    21781 acgaaaaacc gttgaacgtc tacggttact caaaattcct gtttgatgaa tatgttcgcc
    21841 aaatcctgcc tgaagcgaac tcgcagattg ttggcttccg ctatttcaac gtttatggac
    21901 cgcgtgaagg ccataaaggc agcatggcga gcgtcgcttt ccatctcaac actcagctta
    21961 acaaaggtga atcaccgaag ctgtttgaag gtagcgagaa cttcaaacgt gacttcgtct
    22021 atgtgggcga cgtggcagat gtaaacctgt ggttcctgga aaatggcgtt tccggtatct
    22081 tcaacctcgg tactggtcgt gcggaatcct tccaggccgt agcagatgct actctggctt
    22141 atcacaagaa aggccagatc gaatacattc cgttcccgga taagctgaaa ggccgctacc
    22201 aggcgttcac tcaggcagat ctgacaaatc tgcgcgcggc gggttacgac aaaccgttca
    22261 aaaccgttgc tgaaggtgta atggaataca tggcctggct gaatcgcgac gcataagagc
    22321 tctgcatgaa aatactggtg atcggcccgt cttgggttgg cgacatgatg atgtcgcaaa
    22381 gtctctatcg cacgctccag gcgcgctatc cccaggcgat aatcgatgtg atggcaccgg
    22441 catggtgccg tccattatta tcgcggatgc cggaagttaa cgaagctatc cctatgcctc
    22501 tcggtcacgg agcgctggaa atcggcgaac gccgcaaact gggtcatagc ctgcgtgaaa
    22561 agcgctacga ccgcgcctac gtcttaccaa actccttcaa atctgcatta gtgcctttct
    22621 tcgcgggtat tcctcatcgc actggctggc gcggcgagat gcgctacggt ttactcaacg
    22681 atgtacgcgt gctcgataaa gaagcctggc cgctaatggt ggaacgctat gtcgcgctgg
    22741 cctatgacaa aggcattatg cgtaccgcac aagatctgcc gcagccattg ttatggccgc
    22801 agttgcaggt gagcgaaggt gaaaaatcat atacctgtaa tcaattttcg ctttcatcag
    22861 aacgtccgat gattggcttt tgcccgggtg cggagtttgg tccggcaaaa cgctggccac
    22921 actaccacta tgcggagctg gcaaagcagc tgattgatga aggttatcag gtggttctgt
    22981 ttggctctgc gaaagatcat gaagcgggca atgagattct tgccgctttg aataccgagc
    23041 agcaggcatg gtgtcggaac ctggcggggg aaacacagct tgatcaagcg gttatcctga
    23101 ttgcagcctg taaagccatt gtcactaacg attctggcct aatgcacgtt gcggcggcgc
    23161 tcaatcgtcc gctggttgcc ctgtttggtc cgagtagccc ggacttcaca ccgccgctat
    23221 cccataaagc gcgcgtgatc cgtctgatta ccggctatca caaagtgcgt aaaggtgacg
    23281 ctgcggaggg ttatcaccag agcttgatcg acattactcc ccagcgcgta ctggaagaac
    23341 tcaacgcgct attgttacaa gaggaagcct gacggatgcg ggttttgatc gttaaaacat
    23401 cgtcgatggg cgatgttctc catacgttgc ccgcactcac tgatgcccag caggcaatcc
    23461 cagggattaa gtttgactgg gtggtggaag aagggttcgc acagattcct tcctggcacg
    23521 ctgccgttga gcgagttatt cctgtggcaa tacgtcgctg gcgtaaagcc tggttctcgg
    23581 cccccataaa agctgaacgc aaagcgtttc gtgaagcgct acaagcagag aactatgacg
    23641 cagttatcga tgctcagggg ctggtaaaaa gcgcggcgct ggtgacgcgt ctggcgcatg
    23701 gcgtaaagca tggattggac tggcaaaccg ctcgcgaacc gttagccagc ctgttttaca
    23761 atcgtaagca tcatattgca aaacagcagc acgccgtaga acgcacccgc gaactgtttg
    23821 ccaaaagtct gggctatagc aaaccgcaaa cccagggcga ttatgctatt gcacagcatt
    23881 tcctgacgaa cctgcctaca gatgctggcg aatatgccgt atttcttcat gcgacgaccc
    23941 gtgatgataa acactggccg gaagaacact ggcgagaatt gattggttta ctggctgatt
    24001 caggaatacg gattaaactt ccgtggggcg cgccgcatga ggaagaacgg gcgaaacgac
    24061 tggcagaagg atttgcttat gttgaagtat tgccgaagat gagtctggaa ggcgttgccc
    24121 gcgtgctggc cggggctaaa tttgtagtgt cagtggatac ggggttaagc catttaacag
    24181 cggcactgga tagacccaat atcacggttt atggacctac cgatccggga ttaattggtg
    24241 ggtatgggaa gaatcagatg gtttgtaggg ctccggggaa tgagttgtct caattgacag
    24301 caaatgctgt taagcggttc attgaagaaa acgc
//
LOCUS       DBANUU010000072        23888 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000072 DBANUU010000000
VERSION     DBANUU010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.072"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(297..890)
                     /gene="tehB"
                     /locus_tag="ACIE68_002759"
     CDS             complement(297..890)
                     /gene="tehB"
                     /locus_tag="ACIE68_002759"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFX5909048.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(887..1879)
                     /gene="tehA"
                     /locus_tag="ACIE68_002760"
     CDS             complement(887..1879)
                     /gene="tehA"
                     /locus_tag="ACIE68_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFX5909049.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVMSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            2003..2983
                     /gene="ydcK"
                     /locus_tag="ACIE68_002761"
     CDS             2003..2983
                     /gene="ydcK"
                     /locus_tag="ACIE68_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFX5909050.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(2978..3514)
                     /gene="rimL"
                     /locus_tag="ACIE68_002762"
     CDS             complement(2978..3514)
                     /gene="rimL"
                     /locus_tag="ACIE68_002762"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFX5909051.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLSKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(3577..3801)
                     /gene="ydcH"
                     /locus_tag="ACIE68_002763"
     CDS             complement(3577..3801)
                     /gene="ydcH"
                     /locus_tag="ACIE68_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFX5909052.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(3941..5596)
                     /gene="opgD"
                     /locus_tag="ACIE68_002764"
                     /gene_synonym="mdoD"
     CDS             complement(3941..5596)
                     /gene="opgD"
                     /locus_tag="ACIE68_002764"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFX5909053.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(5821..7164)
                     /gene="ydcJ"
                     /locus_tag="ACIE68_002765"
     CDS             complement(5821..7164)
                     /gene="ydcJ"
                     /locus_tag="ACIE68_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFX5909054.1"
                     /translation="MVNSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            7381..8304
                     /gene="ydcI"
                     /locus_tag="ACIE68_002766"
     CDS             7381..8304
                     /gene="ydcI"
                     /locus_tag="ACIE68_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFX5909055.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEDLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(8342..9982)
                     /gene="trg"
                     /locus_tag="ACIE68_002767"
     CDS             complement(8342..9982)
                     /gene="trg"
                     /locus_tag="ACIE68_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFX5909056.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            10381..10530
                     /gene="hokB"
                     /locus_tag="ACIE68_002768"
     CDS             10381..10530
                     /gene="hokB"
                     /locus_tag="ACIE68_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFX5909057.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(10508..10576)
                     /gene="yncP"
                     /locus_tag="ACIE68_002769"
     CDS             complement(10508..10576)
                     /gene="yncP"
                     /locus_tag="ACIE68_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFX5909058.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(10602..10775)
                     /locus_tag="ACIE68_002770"
     CDS             complement(10602..10775)
                     /locus_tag="ACIE68_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909059.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(11020..11550)
                     /gene="cybB"
                     /locus_tag="ACIE68_002771"
     CDS             complement(11020..11550)
                     /gene="cybB"
                     /locus_tag="ACIE68_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFX5909060.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            11739..12740
                     /gene="gap"
                     /locus_tag="ACIE68_002772"
     CDS             11739..12740
                     /gene="gap"
                     /locus_tag="ACIE68_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFX5909061.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(12782..14221)
                     /gene="aldA"
                     /locus_tag="ACIE68_002773"
     CDS             complement(12782..14221)
                     /gene="aldA"
                     /locus_tag="ACIE68_002773"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFX5909062.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVDGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(14418..15218)
                     /gene="ydcF"
                     /locus_tag="ACIE68_002774"
     CDS             complement(14418..15218)
                     /gene="ydcF"
                     /locus_tag="ACIE68_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFX5909063.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(15490..19392)
                     /gene="hrpA"
                     /locus_tag="ACIE68_002775"
     CDS             complement(15490..19392)
                     /gene="hrpA"
                     /locus_tag="ACIE68_002775"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFX5909064.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDCAQMLKVENVQQAWQQWINKLPPARREDEAVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            19593..20198
                     /gene="azoR"
                     /locus_tag="ACIE68_002776"
     CDS             19593..20198
                     /gene="azoR"
                     /locus_tag="ACIE68_002776"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFX5909065.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(20249..21565)
                     /gene="ynbD"
                     /locus_tag="ACIE68_002777"
     CDS             complement(20249..21565)
                     /gene="ynbD"
                     /locus_tag="ACIE68_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFX5909066.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(21555..23312)
                     /gene="ynbC"
                     /locus_tag="ACIE68_002778"
     CDS             complement(21555..23312)
                     /gene="ynbC"
                     /locus_tag="ACIE68_002778"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFX5909067.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRALAFEKMQ
                     SFISHLYANKSQKFDYQHEDCTGPSADRWRLLSGGPVPLSPVDLAYRFMRMAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLVDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVADLHAKGLAIRVVDIAAGHGRYVLDALENEPAVCDILLRDYSELNVAQGQE
                     MIAQRGMSGQVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(23328..>23888)
                     /locus_tag="ACIE68_002779"
     CDS             complement(23328..>23888)
                     /locus_tag="ACIE68_002779"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFX5909068.1"
                     /translation="AGFLILATGQVFMGDPSGFLHTVSAIFWGWIMTVFALSHAAWLL
                     MLPTTNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVI
                     TTMIASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGH
                     GGLLDRIDSLIFTAPVFFYFIRYSCY"
BASE COUNT         6096 a         6243 c         5620 g         5929 t
ORIGIN      
        1 gtttcctgag atgattatat tactttctaa gttatagcaa aaagcgccgc ttcgtgttgt
       61 ggcaaaaaaa gtgttggtga aaataaattt ttcagcccct tttcagacga catagtcaat
      121 gccgaaaaag gggctaagtc aatcatcagt agttaaattt cagtaagtta agctgctaaa
      181 aacttcgact actgtgatta atttcacatc ccttcgataa aaaatatgct taccgaaaag
      241 aagaacagta aatcatctca gcgtggggaa ttttcacgcg ttttgtgctt accgggtcat
      301 tttttacgtg ccagcatcgt ggcgaaacgc agtttaatac gattaccgtt ggcgtcggtg
      361 cggtgcagct cgccgacgtc ttcattgtat ttcaccctct cccagccttc ataatatcga
      421 cgtaattctc cctctttgaa ggcaaacggg aagccgacgg tacatggata atcagcggta
      481 tccatcgccg ccacaatcag gttgtaacca ccaggtttag tgcaacgttg catattggca
      541 atcagccctg ggatggtttt agcctcaagg aacatcagta ccacagtcga aagaataaaa
      601 tcgtactgtc tatcaaatgt gaggttattc agatcaacga cccgggtgtg taaattatcc
      661 agattttcaa tggatttaat gcgctctaca ttggcgatac tcatagcatt tttatcccat
      721 gcgtcaacat cataaccatt ggctgccagg taaagactgt tacgaccatt gccacagccc
      781 agatccagcg ttttacccgg tttaaccact ttcaccgctt ccagtacttc agagtgtgtg
      841 cgggttaatt catatttatc agtaaaatag ttttcgtcac gaatgatcat tctttgtcct
      901 ctgctttcat taaaacggcg cgctcggttc tgactaacaa ttttccctgc atcagaagcg
      961 caaaagtacg gatgagcagt attgcaataa taaaattggt aaagataaac agcggtaccg
     1021 ccagcgtatg gaaaaatcca ttttcgctgc cactccccag atgcaaaccg gtggttgcca
     1081 gtgcagatac gccgaacgag aaactccaga atgaagcatt aaatggctgg gagagatacc
     1141 atggcatcag acgtagcata aacagcagtt gcagcagtcc ataaccaaaa agcattttcg
     1201 ccagcgtatc accctcgccg ccgttgacgc tcaaccaggc actacaggcc accagcgcag
     1261 gagcgagctg aatgccgagt gatgtccgca gtgccgtggg taattctccc gaactgcgca
     1321 gacgctgcaa gatcacaggt tcaaggctta gccatgagaa aacgcctgcg cctaaaaaca
     1381 ccagaccggc gtcggtgtag cccaacgcac cacaggccat tgcgctgata aagttgttgg
     1441 caactgtcgg cagatacagt ccaggcgtgg tagcttcttc agggtgagat ccgcgccata
     1501 atcccgcagt ttgccaggcg gcataagcca actgaaccac gacaccaaaa ctgaacaggc
     1561 acaccgccag tgggcgaaac cacggaacaa aaccaatcgc caccagcatc gtcgttgccg
     1621 gaaacaaact cacaaaactg ctcatcactg gatggcgcac ttctgccagc acgctatgcg
     1681 gaaagcgtat aagtcgggta ataaatgcgc tagttaacaa tccccagatg atcatcgcca
     1741 gaatcaccag cccatcccct aaccagtggc tgacctgcca aacctggctg gcatagcgcc
     1801 aggcaaatcc catccctatc gtccccaaca caataccaaa gtagcctgcc ggcaaattga
     1861 gcactttatc gctctgcatc ttcttactca ttttgtttaa tttataaagt atatttgaaa
     1921 tgcattttat ggaattttct cgagcatagc cagagccgca gaatttgcta cggttatagt
     1981 aagtcacctg tgggaaaacg ctatgcgcaa atatcgtcta agtgaagaac agcgagcctt
     2041 tagttatcag gaagatggca ctaaaaaaaa tgtgttatta cggcagatta tcgccattag
     2101 cgattttaac gatgtgatag caggaaccgc tggcggctgg atcgatcgcg aaacggtgtt
     2161 agcacaggaa ggaaattgct ggatttacga ccagaacgct attgcgttcg gcggagcggt
     2221 gatttccggt aatacgcgca tcaccggcac cagcgtgctg tggggagagg tttacgcaac
     2281 ggataatgtc tggatcgata atagtgaaat cagccagggt gcatatataa gtgacagcgt
     2341 caccattcat gactcactgg tttgcgggca atgtagaata tttggtcacg ccttaattga
     2401 tcaacattct atgatcgtcg ccgcacaagg cttaacgccc gaccatcaac tcctgctcca
     2461 aatttatgat cgcgcaaggg ttagcgcctc gcgtattgtc caccaggcac aaatttatgg
     2521 cgatgccgtc gtccggtatg cttttattga acatcgcgcc gaagtttttg attttgccag
     2581 tgttgaaggt aatgaagaga acaacgtctg gctatgcgat tgcgctaaag tctacggtca
     2641 cgcgcaggtg aaagctggca tagaagaaga tgccattccg acgattcatt acagttcgca
     2701 ggtggcggaa tatgccattg tggaaggtaa ttgtgtgtta aaacatcatg tcttgattgg
     2761 cggaaatgcc gtggtacgtg gtggaccgat tctgctcgac gagcacgttg taatacaagg
     2821 tgagagccgt atttcaggtg cggtgataat tgaaaaccat gtggagttaa ccgaccacgc
     2881 cgtcgttgaa gcctttgatg gcgacacggt tcatgtccgt ggtccaaaag tcattaacgg
     2941 cgaggagcgc attacgcgaa cgccgctggc agggttatta tgaatcgata atacgcgcgt
     3001 ataagttcac atcatcatag gcatcattca ggaactcagc ctgtttcagg caaccttcaa
     3061 ggataaaacc attgcgcaaa gcgacctggt tgctttgcgg attgtccacc cgacatttga
     3121 tcacgaagcg tctaagttca ccagactgag cgtaatgatg aatcaatgcc tgcagcgcct
     3181 gagaaatgat cccctgcccc tgatgagatt cgtccagcca gtagccgatt tcagcagttt
     3241 tactcagtgg ttcaatacga ttaaacgaga taacgccgat aagttcatct tctttgaaaa
     3301 tcatgaacat tttggcatag ccgcgttgat gcaacatcac attaccctgc accgtttttc
     3361 gcgtgtcctc ttcactctga acgaactgcg gccagtttag cgactgctgt aaccaggttt
     3421 tatttttaca gattaactga taaagaggtg tgacgtgact ttccgcaaca gcatgtaatt
     3481 caagtgattc gcttactttt atcgtttcag tcatcttatc tccagtcaat ccatgctgtt
     3541 gttaaaaaag ccgcctggct ggcggcttag gaaagtttac acctctttga cgctctcctg
     3601 ctgcaggatt ttgagcatct catcttttaa ctgtagcttt tgttttttca tgcggaccac
     3661 ttccgcattg taccctcgac cgtcggaacc ttcctttctg gcaatttcat gatcaagttt
     3721 attgtgttta tcgaacaagg acataaagcg aggattttcg tttttcagac gggatattaa
     3781 atctcggtat tctggaaaca tacgcactcc ctgttagtgg ctgacaaggt gtatctgaaa
     3841 tacacaccat cagattactc atttaactta gggaaaaatg cgagcgggat cgtgtttaac
     3901 cagacggcac ctcccgaaga aggtgccgaa gaaaaaacga ttaactcatc acgcggtcgt
     3961 caacatactg acgtttatcc ggcgctggcg ggaaatactg atacagccat gtttcactga
     4021 tagcgtcgcc ctgacaacgc agatacatcc gcatatcgac cggatcagtg gagtccgaag
     4081 tcggatacca gtcaaactga atacgataac catcaatggg ttcaatatag agaatttcga
     4141 tttgcttcgc ttccccactg gaaagcgtaa tcaccggctc aatgcctttt ggtgcggcag
     4201 ctttcagatc accaccaacg aaatcgacgg caaaacgacg cgcccatttt tcgggatagt
     4261 gttcacctgg tgcccaacct tccgggaaac cgcccatgcc ggtacgcgtc gccataacgc
     4321 gcgctaatgg gcaatgaaca ggcggttgcg cactccagta cagacgatac tggaatgcaa
     4381 actcatcacc cgcctttaca gctttttctg gctgccagaa gcagacaatg ttatccagcg
     4441 tttcgcccgt tgttgggatt tccatcaggc cgatggtgcc cttaccccac ttgttacgcg
     4501 gttccaccca cagacttggg cgtttgttat accagcccat aatgtcctga taatgggaga
     4561 aatcacgatc cagttgcaat aaaccaaacc ctttcgggtt gttgtcggtg taagcattga
     4621 actgcaattt ttgcggatta ttcagcggac ggcaaatcca ctcgccgttg ccccgccaca
     4681 tggacagacg atcagagtca tgaatttgcg gatgaatggt gtcgcacatc cgacgttcat
     4741 tagtaccgca gctgaacata ctggtcatcg gcgcaatgcc cagctgttta atgtctttgc
     4801 gcgcatacag gtgattttcc acatccataa tcacctgact tttctcacaa tggatagtga
     4861 acttataggc accagtaatg ctggcgctat cgagcaacgc ataaacggta aaggtagttg
     4921 cccccggttt taccgtatca aaccagaagg cggtaaagtc ggggaactct tctttactgt
     4981 cggtgtaagt gtcaatcgcc aggccgcggg ccgacaaacc gtattgatat gtgtcatcaa
     5041 cggcgcggaa ataactcgct ccgaggaatg atactacatc acggcgcgcc agttcggggg
     5101 ctttaaacac gcgaaaaccg gcaaagccga gatcgctttg cccttctaat tgtttggtat
     5161 caacacctgc atcgttgtat ttgaacaact ccgggcgaaa gtgaatttca cgcgccagat
     5221 gtgttgctgg atctacagaa aacatacgaa cgcggcgacg gaatcccatt cccatatgga
     5281 agaactgagc gtccagttga cggttctcaa cgttatgcca gagcgatttt tcggcgtcgt
     5341 attgaatact gttataagcc tgcggcgtca ttgtcgccag cgtgtcaggt aacggacgag
     5401 gcgcaccacg ccacgctgtt tgcgctaagt cgtgcgccat tgactgtaga atggagaagt
     5461 caaaacgctg ggtttgcccg tcggcaatat cagaatctgc cgcgaatgcc gcctgagaaa
     5521 aaagagaagc aatgccgctg gtaccgcaca cggcggccat agccattgaa cctttaataa
     5581 atcgtctacg atccatacct gaaagtgagt ccttctggtg ttgtgaatgg tcccgcgctg
     5641 gtcgttttca gcggcaagaa agtgcttatt tatgaccgct cactctagac aatatttatt
     5701 aataatccaa ttgttgaact cagagaatat gcataaatcg gcaatttttt tatgtcgatg
     5761 ggatgagcct gattcgtttg taaaatcagg caaatttttg caaaggcgga tggtcagaat
     5821 ttaaagcaaa ccacagcgac gtttactgcg ttcttccgct tcctgataaa gctggaactc
     5881 atccaaaacc ggacaaccca acgcctgctc aaatgcctcg cgactggcat taccgtgatt
     5941 gcgtgcctgc gtttcattac ccagatttga ctggaagatc cccgccgcgc taacgggcaa
     6001 gaaatcttca taggtaatgg gttgcgccgc gacccaacca cgttcaatta agggctgtgg
     6061 atcgtctccg ggatgaatcg cctgacgatg cgcctcacct gaaggcgtca gacggtaccg
     6121 gaaccatgcc agcccttgct gacgcattaa aaactcactg tcaggaaaag tgcggaaggt
     6181 ttcctgtaaa tgcatttggt gagtgagatt atcctgcccg gttccagcgt tacgcagaag
     6241 atcatcatac agttgtcgcc ctttcggcgt taatgccacg ccacgctgct caatttcacc
     6301 aaagcgcgcg gtatgcgtgc cctgtttctg ccccgcaaac aacaccgtct cttccagtgc
     6361 tttaaagctg gtctggcgta gtaaaatcgg tacctcgcgg cgcggcggcc cctcgatgag
     6421 aattttgggt tcaattccgc attcaggcat catcgactgc acccggtcaa tatccagcgt
     6481 acgtggcgtc aggtggttga tatggcatcc aggaaaacag accacatcag caatcaaccg
     6541 atgttcgttg tgcaaggcgc gataggtttc ttcatctacc gttgctgact ggtgccagcg
     6601 aaacgtttcc agggcttcct gcacaaactc ctgtgcctgt gtttcgttaa aaccgccctg
     6661 ctgctcatat tcctctaaca gttgtcgaca acgtggggtg aagatatcgc gctgacgcag
     6721 aatctccgcc gctttctggc gcaaaatttc gttctcgata agctcaaggc ggagtaagga
     6781 ggtaaaaacg cggaagggat tacgcgccag agaagcatca tcaatgggcc gaaatgctgt
     6841 cgaatgtacc ggcacccctg cctgcgagag atcgtaatag ctcaccgggt acatccccat
     6901 aatggcaaac atccgccgaa gagtagcaag ctcttgtgca gtcccaacgc gaatcgcccc
     6961 atgacgttca acattcagtc gcgccagctc gtctgcattt accatttttt cgtgcagttg
     7021 aggattgttt tccagcacag ccagattcac atcagctacc agttccagca gcgtgccata
     7081 ttgcggaact tcttgctggt acatggctga cattgcctgc gaaaactgtt cccgaatctc
     7141 atccgccgtg atgctgttca ccatagtgtc tgcctccagt ggtcagtaat ctggagtgta
     7201 ggtaaccgca ttcactctgg cgggaagaat ttacaaactg tgatctcgcc gcgaaaacat
     7261 caatattatc cattttgctg taacataatt gctttaattg ttaataatat tttgcaatca
     7321 agttatcata atcaaacaac ttcacttgtc agcgacaccg cttcgttttt aacatcgctt
     7381 atggaaaaaa atagtctgtt tagtcagcgc atccgtttgc gccaccttca tacattcgta
     7441 gctgtcgcac aacaaggaac tttggggcgc gcggctgaaa cccttaattt gagtcaacct
     7501 gcgctctcta agacattgaa tgaactggag cagctgactg gcgctcgctt gtttgagcgt
     7561 ggtcgtcagg gggcgcaact taccttaccc ggcgaacaat ttttaacgca tgcagtcaga
     7621 gttcttgacg ccatcaacac tgccggacag tcgcttcatc gtaaagaaga tcttaataat
     7681 gatgtcgtca gggttggtgc actacctact gcggcactgg ggatattacc ttcggttata
     7741 ggtcagtttc atcagcaaca aaaagagacg accttgcaag ttgcgacaat gagtaaccct
     7801 atgattctgg cgggtttaaa aaccggggaa atcgatatcg gcattggtcg gatgtcagat
     7861 cctgaactga tgaccgggct taattacgaa ctgctgtttc ttgaatcgct gaagctggtt
     7921 gtccgcccta atcacccgct acttcaggag aacgtaacgc taagccgggt gctggaatgg
     7981 ccggtcgttg tatcaccaga aggcactgcg ccacgccagc attcagatgc attagtgcag
     8041 agccagggct gtaaaattcc ttcgggttgt atcgaaacgc tgtctgcttc gctatctcgt
     8101 caacttacgg ttgaatacga ctacgtgtgg tttgtccctt ctggcgcggt aaaagacgac
     8161 ctgcgtcatg ccacgctggt ggccctgcct gttccgggac atggtgcagg cgaaccgatt
     8221 ggaatactga cccgcgtaga tgcgacgttc tcttctggtt gccagttgat gattaacgct
     8281 attcgaaaat caatgccgtt ctgaaaggtg aagggatctg tcgatccctc cttgaacatt
     8341 ttcacaccgt agcgaaacta actggttcac ccgctccgcg aggttctgcc gacacagaat
     8401 gtttgttcag acggaatacg tccaccgcct cagttaatcg tgccgcctgt tcttcaaggg
     8461 acaccgccgc tgctgaggcc tcttctacca gagaagcatt ctgttgcgtc accttatcca
     8521 tttcagaaat cgcctggcta acctgagtta tgcctctact ttgttcatcc gaggcggcgg
     8581 cgatttcctg catgatatgt gtgacactcg cgacggcatc aacaatagtg ctcatggttt
     8641 tccctgccgt tgccacctca tccgacccca ggtcaattaa cctgactgat tcactgatca
     8701 agccttcaat ctctttcgcc gcttgggcgc tgcggcttgc gagtgtccgt acttcgctgg
     8761 cgacaacggc aaatccacgg ccttgctcac ccgctcgcgc ggcttcaacg gcagcattca
     8821 gtgccagaat attcgtctgg aaagcaatac tgttgatgac ggcggtgatc tcggaaattt
     8881 tcttcgaact tgtagagata gcgcccatcg tttttactac accggaaacc gtctgcccgc
     8941 catcgctggc tttaatagaa gcctcttgcg ccagtttgct ggcatgatgc gcgttatccg
     9001 cattctgttt caccgtcgca gtgagttgct ccatgctggc ggcagtttgt tcgatagccg
     9061 ccgcttgttc ttcggtgcga gatgacaggt ccgcattgcc agctgaaatt tcgctggtgc
     9121 cacgataaat ctcttccgcg ccctgtcgaa cagtccctac tgtcatcccc agtgaatgct
     9181 gcatttgctg taaatgacga cttaagcgac cgatttcatt acgacccgca ggttcatcat
     9241 tcatcgtcag atcgccactg gcgatttttt caatccgttg tgcggcatgt tgcagtggac
     9301 gaatgacgat ccgacgtagc accataaatg ttatcagcgt catgaccagg gcaagcacaa
     9361 acgcgccaat catgaacatc ccacccaggc gggtgcgctg atgggccagt tccgccagtt
     9421 ggttggctct ggtgctacgt atcttaacgg ctttgttcaa aatatcggta taagcattat
     9481 ccagcggtcg gatctgctca ctttcatgat tgataatcgc ttcaaacatg ccatttttgg
     9541 catatttcaa cataggttgc atacccgtga tataagcctg aaagcgttga tttaattcag
     9601 tgtcgagggc ttcatcagca ggtgttttca ccggccgatt ctgataagca cgataacctt
     9661 gctgcgactg tttaatctcc gattcggctt gcgcaatatt gcgcttcatt gcttccattt
     9721 ccgcaatacg actcgcagcc ccggcctgaa tcatattgat ccgggctgaa cgcaaaaaat
     9781 ctgaactgtt cgctaacccg gtccgaatct caatctccgc agtaacatca cgctgatcgc
     9841 gatcggcctg ccagaggaaa tagccagcca gagcagaact taatgcgaat agaactaaga
     9901 taccgccaag aatgcaggca aataacggaa ccagcctgat gtgatgcaaa aaacctaatc
     9961 gatgtgaggg agttgtattc attaccgttg tctctcgtcc aggtttactc ctgatagaaa
    10021 gtcatcggca cttgacggta attacttatg accaaaaacc tctttgcgtc acatttttac
    10081 aacaattatt gcagagaaaa ttaatgctat tggaatgaag agagtaacga atggctaaac
    10141 tggctaaccc gaatgacgaa aaattctgga aaacagcacg caatttcgtg aagtgattat
    10201 tttcgtccct aaggaaacct cataggatgc ctgcgggtgc ctgagacttt ctgttttcag
    10261 gctaaccggg aatcagaaag acgaaggccc cgaccgatat ttctatcaac cgaggccaac
    10321 gaatgaacct agcaattcag atcttagcct cttacccgcc atccggcaag gagaaaggct
    10381 atgaagcaca accctctggt ggtgtgtctg ctcattatct gcattacgat tctgacattc
    10441 acactcctga cccgacaaac gctctacgaa ctgcggttcc gggacggtga taaggaggtt
    10501 gctgcgctca tggcctgcac gtccaggtaa gggcaagcgc ggggattttc cccgcgcatc
    10561 tgactagcag attcattttc tcatggcacc ccttttacac ttcagttagt gcatgttttt
    10621 ttcgaggcac taatagaacc atcattacag acaaatttgc cgcctgccgt acagtgtgag
    10681 atcccccctt tcgaaccaga acagggtttg cgcccggcat cggcataaaa ggaaacaagc
    10741 gttcccataa acaaaataag tgccagtttt ttcatataac ctccatggtt tatcgaatct
    10801 tatgttcgat ttgaacgtta tacgctggtt tattttgata ccgttgatga gatcaaagat
    10861 cgggtgaatt gattaaaaat aagccagatg gacgtatggg caaggattat ggttttattt
    10921 gtcatacaaa taaatataat aggcgcttcc gatgtagacc cgtattcttc gcctgtacca
    10981 cgggtcggtt ttagtacagg cgttttcttt aaatatcctt caggaacgtt tacgcggcat
    11041 catgcgtaga agtgtgttgt ccttccagaa atagtggtgt gccagtgcag ccgcagcgtg
    11101 caacccgatg acaaaatatc ccagattcgc cagcgtctca tgccacgact ttaagctatc
    11161 aacccgttcg aaattggcct ctgaagcgta aggcatcgtc aaaccaaacg caaaccacgg
    11221 gttgccccgg ttatacatca tcaccaaacc aatcactggc agcgcaataa acaggagata
    11281 aatcaccaaa tgtcccaaat gcgccagtcc cgtcatcatc ggttttggct taggtataat
    11341 cggcggggtt gggtatttca gtcttaacaa aagacgaacc accatcagca cgagaattga
    11401 gataccacag gaaacatgaa ccatgttgat gagtggccga tcgctacgtg ggaagaaacc
    11461 acgaaactcc attgcgcaat acgctgcgat aaccagcaaa aagaccagcc agtgaatgct
    11521 gatttgtaac cttgagtatt tattttccat aacatttcct gctttaacat aattttccgt
    11581 taacataacg ggcttttctc aaaatttcat taaatattgt tcacccgttt tcagtacgtt
    11641 acgtttcact gtaagaatgg agattgccca tcccacaatc ctggtctaag cctggaaagg
    11701 atcaattttc atccgaatgt tcctgacagg agaaaaccat gagtaaagtt ggtattaacg
    11761 gttttggtcg tatcggtcga ctggtgttgc gtcgattact tgaagtcaaa agcaacatag
    11821 acgttgtcgc tattaatgat ctcacttccc caaaaattct cgcctacctg ctgaaacatg
    11881 attcaaacta cggaccattc ccctggagcg ttgattttac ggaagattca cttatcgttg
    11941 atgggaaaag tatcgcggtt tacgccgaaa aagaggctaa aaatattccg tggaaagcga
    12001 aaggtgcaga aatcattgtc gaatgtactg gcttttatac ctccgccgag aaatcgcagg
    12061 cgcatcttga tgctggtgcg aagaaggtgt tgatttccgc ccctgccggt gaaatgaaaa
    12121 ctatcgttta taacgtcaat gacgacactc tggatggcaa cgacaccatt gtttccgtgg
    12181 cgtcatgcac cactaactgt cttgcgccga tggccaaagc cttgcatgac agtttcggga
    12241 tagaagtcgg cacgatgaca accattcatg cctatactgg cacccagtca ctggtggatg
    12301 gcccacgtgg taaagattta cgtgcttcac gcgcagcggc agaaaatatc attccccaca
    12361 ctacgggggc ggcaaaagcc attggtctgg tgatcccgga actgagcggc aaactgaaag
    12421 gtcatgcgca acgcgtgccg gtgaaaacag gttcggtcac tgagctggtg tccattctcg
    12481 gaaaaaaagt gactgccgaa gaggtgaata acgcacttaa acaagcaacc accaataacg
    12541 agtcatttgg ttataccgat gaagaaatag tctcttccga tatcattggc agccatttcg
    12601 gttcggtgtt tgatgccacg caaacggaaa ttaccgccgt gggcgattta caactggtga
    12661 aaacggtcgc ctggtacgat aacgaatatg gcttcgtcac gcagcttatt cgcaccctcg
    12721 aaaaattcgc taaactctga cgcgcacagg cggaggaaaa aacctccgcc tcttttactc
    12781 attacgactg taaataaacc acctgggtct gcagatattc atgcaggcca tgtttaccat
    12841 ctgcgccgcc aataccggat ttacgccatc cggcgtggaa gccttgcata gcttcgaagt
    12901 tttcacggtt gatgtaagtt tcaccaaact tcagcccttt aatggctttc atcgcgacgt
    12961 tcagattttg ggtatagatt gatgaggtca ggccgtaatc actgtcatta gccattgaga
    13021 tagcatcttc cagcgtgtca aatgcgacca ccggcagaac cgggccaaag gtttcctcat
    13081 gcataatcga catttcctgg cgaacatcca gcagcaatgt cggcggataa taatatcctt
    13141 tcccgtctac tgctttgcca cccaacgcca ctctcgcccc ttcttctact gcgcgcgcca
    13201 ctttttgctc gaccctttcc agcgccgcgg cgttaatcaa cggccccatc gcaatgtcgt
    13261 tgcgttcagc ggggttacca aattgaaccg cctgcatcgc ttcacccagc cgattgacga
    13321 actgatcata aatgcctttc tgtacataaa cacgttctgc acagttacac acttgcccac
    13381 tattaatgac gcgtgaatca acgatggctt tgactgccag ttcaagatcg gcatcgtcca
    13441 ttacgatagc tggtgcttta ccacccaatt ccaggcacac tttggtgatg tttttcgccg
    13501 cagtcgccat aatcttctca cctgcagaga cgctgcctgt catactgacc attgcgacct
    13561 ttgggttacc cgccagttct tgcccaacgg tttcaccacg ccccagtaca aggttaaaca
    13621 cgccgcgcgg aaggcctatt tcatcgacga ttttggcgaa tgcaatcgca ttgtttggcg
    13681 taaattcact gggtttaatg acgatggtat taccggtcaa aagagcggga gccattttgc
    13741 gggcaatgag gaagaacggg aagttccacg gcagaatgcc ggtagtcaca ccaagcgcac
    13801 gtttaaacaa aagaatattt tctcctggac gatcgctttg aataatctcg ccctcgtaac
    13861 gccgtgccca ctccgccatg taatcgatat agtcggcagt aaaagccact tcgacttcag
    13921 ccagctgctg gatcttgccc ccttcttcaa caatcagcgc actgatttca ctggcgcgtt
    13981 cgcggatgcc ggcggagatt ttgcgcaacc aactggcgcg ttcaatagca ggcaacgctt
    14041 cccattctgg ttgtgcacgt tctgctgcat cgattgcctt acgggcatcc tcggcctgac
    14101 catcgggtat gcgggaaatg acagcctctg tagcagggtt taccacatca atccatgcgt
    14161 ctccacgcca ggtaacaaac tgtccatcga tatacatagg atgttgaacg ggtactgaca
    14221 tgggcgactc ctgtgattta tatgtttgtt ttcggtgaat gcattgttaa ttaacatcaa
    14281 tcatcagaga gacggcatcg tgaaaggaca attctgtgaa gggcttcata aaaacaagtg
    14341 taaaaggaat aaaaggcaga aataaaagtc aggagtcatg cccgtttgca aacaagtggt
    14401 tcacaaacgg gcaattttta acgtaacgag cgactttcca tcgcctcgat gagcaccgca
    14461 tcatgtttca gcgtttgcca tgcatggatg acttctgccg gaaaatcaac gtgaacgata
    14521 aaatctcgcc cgcggggacc gtagccatcg ctatcatcgc gtaaacgcgg cagctcgcca
    14581 gtgagtagtg agagataacg ctcaaccggc cataatcctt gtaactgatt aataaagatt
    14641 acactgtctg cgttatttcc taactgagga acgaatccgg gataacttaa ccagcgtggt
    14701 gcatcgggat tgtccccagt catacggcgg aacgtcgcca tcgtgcgccg ctgcatggtg
    14761 gggtcctgaa caacgatagc cgtatgaact cgttctacgg cctgattcaa tagcgcgatg
    14821 ctaaagcgtg cgttttcacc gcagtttgtt gactggtctt caatccagat tttttcatgc
    14881 ggaatgtgcc agaactgatg agcgatatcc gccaggatgg tcgcttctgc tctgccagtg
    14941 gtgcggatag tgttgtagtg cggatgctgt gcgatggcgc tatacaaaaa agttgtcgag
    15001 tgaccgatac caccactaat cagtaaagga atttgctgat cgcgagcaat cttacatgcc
    15061 gcgtcgatag tcggcataac cgcattgcct gcaaggatca cgcaatcggc ctgatacggc
    15121 acctcaccgg agaaatcatc ctgcgccagc cactgtccga taacatttat ggcatctata
    15181 gttgccggcg aaagcgtcgg aaacggggta atgttcatgg ttcctcctta tcgttccctt
    15241 aagaatactg cgtgagataa tagtgagaag cggagcactc tgaaaggagg aagtcgtatg
    15301 tccttaaccg ccactccgca agcctgacat atcttttaag gaaatgctgt agataaacat
    15361 tattcatctt gcagcagaga attgtatttt tctgaattcc tgaattttta cccttaccgt
    15421 tttagaatta ataaaaaacc cggcgctaaa tgcttaccgg gttttttctt tatcaggcaa
    15481 atagcagggt taaccgctaa tctgctccat cgcctgcaaa atacgcttat ctgaaatcgg
    15541 ataaggcgta ccaagttgtt gagcgaagta actaacgcgc aactcttcta tcatccaacg
    15601 gatctctttc acggcttcat cctcacgacg tgcgggcggc agtttgttga tccattgctg
    15661 ccacgcctgc tggacgtttt cgactttcag catctgagca cagtcgcgat gtggatcaac
    15721 cgccagtttt tccagccgtt tttcaatcgc ctgcaaatat cgcagcgtgt cgcccagccg
    15781 tttgaagccg ttaccagtga caaaaccgcg atataccaac ccgcccatct gcgctttaat
    15841 gtcagaaagc cccagtgcca tggtcatatc cacccgccct ttcagacgtt tgttgatatt
    15901 gaacaccgca gtgaggattt gctcgacctg cttcgcaata tccaccaccg tgtcattcag
    15961 ttcggcgcgc actttttcat gcagcgcagc aaagccttct tccgtccaga ccgggccacc
    16021 attggcgtcg atcaattgat ccacaccgca ggagatacag tcgtcgatca gctccagcac
    16081 tttgccatac gggttaaagt acagtcccag cttggctttg ttcggtaact tttcatgtaa
    16141 atatttgatt ggcgatggaa tattcagcag cagtagacgg cgaagaccgt tccacattgc
    16201 ctgcttttgc tccagcgggt tatcaaacag tttgatcgcc acactgtcgc gctcatccac
    16261 cagcgccggc cacgccttca ctttgtagtt gccacgcttc tgttcgtagc tttccggcag
    16321 ctgaccaaaa ctccagatat gtaagccgct ctgctcgata ccgtcatccg ccaccgcaga
    16381 tagcgtttcc tgcactttgc ctttcagcgc atctttcaga tcttgtagcg agcgcccttc
    16441 ttttagcttc ttgtttttgt catccaccac gcggaaggta attttcaggt gatcaggcac
    16501 ctgatcccag tgccagtctt cgcggtcaac ggtaacgccg gtcatccgcc gtaactcgcg
    16561 ctcaaggctg tcgagcaacg gtaactccag cggtttgacg cggcctaaaa acgcttcggc
    16621 atagtttggc gcgggtacaa aattacggcg taccggtttc ggcaacgatt taatcagagc
    16681 aatcaccagt tcgcggcgca gaccggggat ctgccattca aacccgcttt cctcaacctg
    16741 gttaagtaac ggcagcggaa tatgaacggt cacaccgtca gcatccgcgc cgggctcaaa
    16801 ctgatagctc aaacgcagct tgagattgcc ctgatgccag aagttcgggt aatccagctt
    16861 gctgattttt tctgcgccct ctttgatcaa catgcttttt tcaaagttga gcaaatcagg
    16921 ggtttcgcgg ctgacttttt tccaccagct gtcgaagtga cgagcggaga ttacatcgtg
    16981 gctgatgcgc tggtcgtaga actcaaacaa cgtttcgtca tcaaccagaa tatcgcggcg
    17041 acgtgatttg tgttccagtt cttccacttc agcccgcagt ttcaggtttt cacggaagaa
    17101 tgcgtgacgc gtctgccagt caccttccac cagcgcgtgg cgaataaaga gttcacgaca
    17161 taacgccgga tcgatctggc tgtagttgac cttgcgcgcg gcaacaatcg gcaacccgta
    17221 aacggtgact ttttccgttg ccatcaccgc gccctgcgcc cgttcccagt gcggttcgct
    17281 gtaggtgcgt ttaatcaaat gctgagcaac tggctccacc cattctggat cgatgcgcgc
    17341 cgcaatgcgc ccccacaggc ggctggtttc taccagttcc gccaccatta cccatttcgg
    17401 cggctttttg aataaaccag aaccggggaa gatggagaaa cgcgcgttac gtgcaccggt
    17461 atattcctgt ttatcggcat ctttcatgcc gatatgggaa agcaaaccgg tcagtaaggc
    17521 gatgtgaatt tcgcgatact ccgccggttc gctgttgacc ggaatgccaa gttctttcac
    17581 cacctggcgc aactgggtgt agatatcctg ccattcgcgc acgcgcagat agttgagata
    17641 atcggtacga cacagtcgac ggaaggcgtt agaagaaagc gccttttgct gctcgccaag
    17701 ataattccac agattcacaa acgcgaggaa gtcagactct ttgtcgtgga agcgacgatg
    17761 tttttcgtcc gatgcctgct gcttgtccat cggacgctcg cgcggatcct gaatggagag
    17821 cgcggacgtg ataatcatcg cctcacgcac gcagccatgt ttttgcgctt ccagcaccat
    17881 acgcgccaga cgtgggtcga caggcaactg cgagagctgg cgaccgagcg gcgtcagttt
    17941 ataggcgctg gcctgttcat ccgtggtgat cgcgcccagt tcttcgagca gacgcacgcc
    18001 atcctggata ttgcgtttat ccggtgcttc gacaaacggg aacgcagcga tatcgcccag
    18061 ccccagcgcg gtcatctgca aaataaccga ggccaggttg gtacgcagaa tctccggatc
    18121 ggtaaactcc gggcgcgaga ggaaatcgtc ttcggaatag agacgaatac agatcccttc
    18181 ggacacacga ccacagcggc ctttacgctg attggctgaa gcctgtgaaa tcggctcaat
    18241 cggcaaacgc tgcactttgg tgcgatagct gtagcggctg atacgcgctg taccggggtc
    18301 gatgacgtat ttaatccccg gtacggtcag cgacgtttcc gcgacgttgg tcgccagcac
    18361 aatgcgccgt ccgctgtgcg actggaacac gcggttctgt tcgctgttcg aaagccgcgc
    18421 ataaagcggc aagatttcgg tatggcgtaa gttcagcttg ttcagcgcat cggcggtatc
    18481 gcgaatttcc cgctcgccgc tcataaagat cagaatgtcg ccggggcttt cctggcttag
    18541 ttcgtctacg gcgtcaaaaa tcgcctgcaa ctgatcgcgc tcggtgtcat cggcttcttc
    18601 aacaatcggg cgatagcgca cttccaccgg ataggtccga ccggagactt caataatcgg
    18661 cgcattatta aagtggcgcg aaaagcgttc cgggtcgata gtcgcggaag tgataatgat
    18721 ttttaggtca ggacgccgcg gcagcaactc tttcaaatag ccgagcagaa aatcgatatt
    18781 caggctgcgt tcgtgcgctt cgtcaataat gatggtgtcg tactgcatca gcaggcggtc
    18841 ttgctggatc tccgccagca ggatcccgtc ggtcatcagc ttgaccatcg tgttatcact
    18901 tacgtgatcg ctgaaacgca ctttgtaacc gatgcaaccg cccggctccg ttttcagctc
    18961 ttccgcaata cggttcgcca ccgttcttgc cgccaggcga cgcggctggg tatggccgat
    19021 cagcccttta atcccgcgcc ccagctccat acagattttc ggtaactgag tcgttttacc
    19081 agaacccgtt tccccggcga cgatcaccac ctggtgatca cgaatcgctt cgagaatgtc
    19141 ctgttttttc tgactaaccg gtaaattgtc aggataagta atttccggtc gtgccgcttc
    19201 acgcagcagg actttccctg ccgcctggtc aatctctttc gccatctcct ggaaaatggc
    19261 ctgttgtgca tcaggatttt taaccttctt cacaccgtgc agacggcgag aaaaacgcag
    19321 tctgtcgcgc agcatcagcg aatccagccg ctgctgcaag gccgtaaagg tcaatttttg
    19381 ttgttctgtc ataacgttag tgggcagtaa tctgcctgat taattttctt ttaaatgata
    19441 gatatagagt accacatcgc ggctttctat gcgttgttca ataaattcga acataggctt
    19501 cgatttattg cgctatctct gtggcaggat tgtgaataaa gtgtcaacaa gcaacggggc
    19561 atcgcccatt caaacatcta taaggaaaca ccatgagcaa ggtattagtt cttaaatcca
    19621 gcatcctggc agggtactct cagtctaatc agttgtccga ttattttgta gaacaatggc
    19681 gcgaaaagca ctccgctgat gaaatcaccg ttcgcgacct ggctgcaaat ccgattccgg
    19741 tactggatgg cgaactggtt ggcgctctgc gtccgagcga tgcgccgctg actccgcgtc
    19801 agcaggaagc tctggcgctt tccgatgagc tgattgccga gctgaaagcc cacgacgtta
    19861 tcgttattgc ggcaccgatg tataacttca acatctcaac tcagttgaaa aactattttg
    19921 acctggttgc acgcgcaggc gttactttcc gctataccga gaacggtccg gaaggtctgg
    19981 taacgggtaa aaaagccatc gttattacca gccgcggtgg gatccacaaa gatggaccaa
    20041 cggacctggt gacgccgtat ctgtccacgt tcctcggctt tatcggcatt accgatgtga
    20101 aatttgtctt cgccgaaggg atcgcatacg gtccggaaat ggcagcgaaa gcgcagtctg
    20161 acgcgaaagc ggccatcgac agcattgttg ctgcataacc ggacacgatc ttgatgtgga
    20221 ctgtagttca gtccacatct caatccactt acctgttttc ccataatttc agcatcgctt
    20281 tgtgatcttc cttaagcaca atatgcgaac gtctggctcg aatataacta atcgcttcat
    20341 caacggtttt acagtgtccg taacataaca accatgccgc cactaccagc gcactgcgcg
    20401 ataatcccaa cgcgcaatgg accagaacgc tgccttgctc ttcgcgtaat gtttccagca
    20461 tcgccacggc ctgtcggagc tccccctctt ccggaaccac cagatccaac atcggtacac
    20521 agaaatagag tcgatctttt gtcgctcgtc cccgagggaa ttcaaaggtg acgtccagaa
    20581 ccgcattctg tgccggaata tgtcgtggaa acgcccctaa ataaacacca gcagtaattt
    20641 tgctcaccgg ctccaggcga cgacaaaacc aacgcataga cagccacatc ccgatgcgcc
    20701 agggcaatgt cagccagtaa acggcgggcg ggagtttccc ctgactatct ttccctgttg
    20761 ttatcgcgcc aagcccaccg tacccacggc caataatgag tagcgataat actggccaac
    20821 ataaccagac tgaccaccat aactgaatca tcaccattag ctccatcaac acaatgcacg
    20881 agcccgcgcc tacgacatat ggcagtgcta ttttgattcg acgttgatca ggtttctgat
    20941 aattccaacg acgatcgacg ggcaccatcc agtcaatcaa catacctacc gccagccctg
    21001 tgatgacatc aataaaatga tgctgccagg tcgtcagagt agaaatagcg atgagtaaaa
    21061 accatccgcc acagacttta cgccacctct cagccagatg ctgacgaaag tgacgccaaa
    21121 gtagccagca gagaataata tgcagcgaag gagactggtt ataaggcaga tcaaacagtt
    21181 caagttgcga aaatagccat cccgtcaccc cactcacttc aggacggata aaactaaact
    21241 tcagcggata gagcaaaaaa ccgcagcagg ccattaccgt tgccagaata agccggtgga
    21301 caagtcggcg ctgttcgaat gtcgtgctac aaacgaacag cgaaaatcca tataaaagat
    21361 ccagactcca gtaaggaaca atagtccagg gaagaaaagg gatcgccgtt tcccaaccga
    21421 atacctgact ggggatatca tggctgttaa ggtcctgaac cgcggtgaac tgattaagag
    21481 atccataggt gaagaagaaa aacggggcca gcaacaataa ccagccagcg ccttgtagca
    21541 atacgttccg acgttcagtt atcacgacgc accgccatcg aaaccgtaaa aataccccac
    21601 tcatcaatcc gttgtgtgca tttatcaaat ccggcatcac gcacgagtga atccatctcc
    21661 ccttgcgaac gcacgcgcat cacccacggt ttaccatctt tatgactggt taacaccccg
    21721 gcaatcagct ccagttgtgg gtgccacggc tgcccggtgt agatgagaat gccacccggt
    21781 tcgatggcat tggcaagacc tgcgagggag ttttttacct gctcattttc gggaaaaagc
    21841 tcatacaggc cagagacaat cgccagcgta ggccgcggag ttaacgcgct gagttcctcc
    21901 gggttaaacg catcgccctg ttcaaaacgc acctgcccag acattccccg ttgggcaatc
    21961 atctcttgcc cctgtgcaac atttaactca ctgtaatcac gtaacaaaat atcgcatacg
    22021 gcaggttcat tctccagcgc atccagtaca tagcgcccat gccctgcggc aatgtcaacc
    22081 acgcggatgg ctaaaccttt ggcgtggaga tcggcaacgg cttgtttaat cagcatttgt
    22141 aaatgggttt tgcgctggcg aataccgcgc cagccaacac tgttcaggta gattttgtcg
    22201 actaaacgcc cgaatgcgtt actaccttgt ggttgatttt gatagacata atccagcgaa
    22261 ctgcctgaat caaagccggt gctcattccg agatgcaggc ccgcagagtg cgccccgaac
    22321 aatttcatcg ccatacgcat aaagcgatac gccaaatcaa ccggcgataa tggcacgggt
    22381 ccaccagaaa gtagccgcca tcgatccgct gatggtcctg tgcagtcttc atgctgataa
    22441 tcaaattttt gcgatttgtt agcatataaa tgactaataa agctttgcat tttttcaaat
    22501 gccagcgccc tgttctcttc acccaacgtg tcgtgataaa agcctggcag cagatgcagc
    22561 tctttgagag ggctacgtaa acgctgataa aaatcgatct gcggttggcg atgcaccaca
    22621 tagtcatcgc ctgatatcag aagttgcgtg gggagcgtaa tcgccgccgc atcgctaaca
    22681 atacgttcag acgttttata gagatcgagc aagatattga cggcaatcgc ccgtgtgatc
    22741 agcggatcat tattgaaact cgccacccgt tgccgatcgt gggtcaaata gcgtcctttc
    22801 acataggaat taataaaaaa cagaccacgc agacgatgcc ataacgccag cgcaggacgt
    22861 gccagcggca catacaattt aaccttaaag gccggagaag ccagcaccag cccgcgaatt
    22921 gcaggcgcat aatcatgaac ccatgtggca accagcactg cgccgacgct ttgcgcgatc
    22981 acaaccacct cttccagtcc aacctggctg tcgctggcag caaaacggac aaattcatcg
    23041 acatcctgca ctgaacgcgc aagagatggg ctataaccac gcggccccga agtttttcca
    23101 tgcccccggg catcccatgc ataaaaagca gtatctggca tcgccagttc atcaacgaga
    23161 tgttgtagac gaccagaatg ttcatgaccc cgatgaaata agacgatgac ctttttcgcc
    23221 ccgggctgta aagcgggcca gtgccgataa aacaacgctg tattatcact ggtggtaaaa
    23281 aaatgttccc cagggatgcg tgaattttcc attttctcta ttttccttca gtaacagctg
    23341 tagcgaataa aataaaaaaa taccggagcg gtgaaaatta atgagtcaat cctgtcaagg
    23401 agtccgccat gtcctggtaa tagttttcca ctatctttaa caccaatatc tcgtttgatg
    23461 gctgacatca cgacatcgcc gcaaaaacca ctaataccaa ttaacaaacc cgctaataat
    23521 gcctgtaatg tatttagcgg tgtcagcaac ggtccaataa tcagtgacgc aatcatggtg
    23581 gtgatgacgc cccccaccag accttccagc gtttttcccg ggctgacttt ggggaccact
    23641 tttcttctgc cgcaggattt tccccataaa tactgtgcaa tatcgtttga ctcggttaat
    23701 gccaaaagaa ataacaccag taacgccccg ccctggatat ttgtggttgg caacattaat
    23761 aaccaggcgg catgactcaa ggcgaaaacg gtcattatcc agccccaaaa aatggcgctc
    23821 acggtatgca gaaaaccgga ggggtccccc ataaaaactt gcccggtggc taatattaga
    23881 aacccagc
//
LOCUS       DBANUU010000073        23821 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000073 DBANUU010000000
VERSION     DBANUU010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.073"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            9..83
                     /gene="tnaC"
                     /locus_tag="ACIE68_002780"
     CDS             9..83
                     /gene="tnaC"
                     /locus_tag="ACIE68_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFX5909069.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            304..1719
                     /gene="tnaA"
                     /locus_tag="ACIE68_002781"
     CDS             304..1719
                     /gene="tnaA"
                     /locus_tag="ACIE68_002781"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFX5909070.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1810..3057
                     /gene="tnaB"
                     /locus_tag="ACIE68_002782"
     CDS             1810..3057
                     /gene="tnaB"
                     /locus_tag="ACIE68_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFX5909071.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3189..4364
                     /gene="mdtL"
                     /locus_tag="ACIE68_002783"
     CDS             3189..4364
                     /gene="mdtL"
                     /locus_tag="ACIE68_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFX5909072.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4339..5298
                     /gene="yidZ"
                     /locus_tag="ACIE68_002784"
     CDS             4339..5298
                     /gene="yidZ"
                     /locus_tag="ACIE68_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFX5909073.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWSLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5455..6204
                     /gene="yieE"
                     /locus_tag="ACIE68_002785"
     CDS             5455..6204
                     /gene="yieE"
                     /locus_tag="ACIE68_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFX5909074.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPLHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            6226..6792
                     /gene="yieF"
                     /locus_tag="ACIE68_002786"
                     /gene_synonym="chrR"
     CDS             6226..6792
                     /gene="yieF"
                     /locus_tag="ACIE68_002786"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFX5909075.1"
                     /translation="MSEKLKVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6846..8183)
                     /gene="adeP"
                     /locus_tag="ACIE68_002787"
     CDS             complement(6846..8183)
                     /gene="adeP"
                     /locus_tag="ACIE68_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFX5909076.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALMFILKIVFIDAH"
     gene            8350..9014
                     /gene="yieH"
                     /locus_tag="ACIE68_002788"
                     /pseudo
     CDS             8350..9014
                     /gene="yieH"
                     /locus_tag="ACIE68_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
     gene            9081..9554
                     /locus_tag="ACIE68_002789"
     CDS             9081..9554
                     /locus_tag="ACIE68_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909077.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELYENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9603..10190
                     /gene="cbrC"
                     /locus_tag="ACIE68_002790"
     CDS             9603..10190
                     /gene="cbrC"
                     /locus_tag="ACIE68_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFX5909078.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKRGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10252..10974)
                     /gene="yieK"
                     /locus_tag="ACIE68_002791"
     CDS             complement(10252..10974)
                     /gene="yieK"
                     /locus_tag="ACIE68_002791"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFX5909079.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(10989..12158)
                     /gene="yieL"
                     /locus_tag="ACIE68_002792"
     CDS             complement(10989..12158)
                     /gene="yieL"
                     /locus_tag="ACIE68_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFX5909080.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(12185..13801)
                     /gene="bglH"
                     /locus_tag="ACIE68_002793"
     CDS             complement(12185..13801)
                     /gene="bglH"
                     /locus_tag="ACIE68_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFX5909081.1"
                     /translation="MFRRNLITSAVLLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13887..15281)
                     /gene="bglB"
                     /locus_tag="ACIE68_002794"
     CDS             complement(13887..15281)
                     /gene="bglB"
                     /locus_tag="ACIE68_002794"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFX5909082.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(15300..17177)
                     /gene="bglF"
                     /locus_tag="ACIE68_002795"
     CDS             complement(15300..17177)
                     /gene="bglF"
                     /locus_tag="ACIE68_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFX5909083.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLDEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTSLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(17310..18146)
                     /gene="bglG"
                     /locus_tag="ACIE68_002796"
     CDS             complement(17310..18146)
                     /gene="bglG"
                     /locus_tag="ACIE68_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFX5909084.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILG
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVHK
                     EH"
     gene            complement(18432..19157)
                     /gene="phoU"
                     /locus_tag="ACIE68_002797"
     CDS             complement(18432..19157)
                     /gene="phoU"
                     /locus_tag="ACIE68_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFX5909085.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(19172..19945)
                     /gene="pstB"
                     /locus_tag="ACIE68_002798"
     CDS             complement(19172..19945)
                     /gene="pstB"
                     /locus_tag="ACIE68_002798"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFX5909086.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(20036..20926)
                     /gene="pstA"
                     /locus_tag="ACIE68_002799"
     CDS             complement(20036..20926)
                     /gene="pstA"
                     /locus_tag="ACIE68_002799"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFX5909087.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(20926..21885)
                     /gene="pstC"
                     /locus_tag="ACIE68_002800"
     CDS             complement(20926..21885)
                     /gene="pstC"
                     /locus_tag="ACIE68_002800"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFX5909088.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(21972..23012)
                     /gene="pstS"
                     /locus_tag="ACIE68_002801"
     CDS             complement(21972..23012)
                     /gene="pstS"
                     /locus_tag="ACIE68_002801"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFX5909089.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(23260..>23821)
                     /gene="lpfD-O113"
                     /locus_tag="ACIE68_002802"
     CDS             complement(23260..>23821)
                     /gene="lpfD-O113"
                     /locus_tag="ACIE68_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFX5909090.1"
                     /translation="GKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSC
                     EINNLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYA
                     TEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAA
                     PASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         5987 a         6013 c         5667 g         6154 t
ORIGIN      
        1 agtgcattat gaatatctta catatatgtg tgacctcaaa atggttcaat atcgacaata
       61 agattgtcga tcaccgccct taatttgccc ttctgtagcc atcaccagag ccaaaccgat
      121 tagattcaat gtgatctatt tgtttgctat atcttaattt tgccttttgc aaaggccatc
      181 tctcgtttat ttactagttt tagtaaatga tggtgattgc atatatatct ggcgaattaa
      241 tggatattgc agatgtaata ttcacaggga tcactgtaat taaaataaat gaaggattat
      301 gtaatggaaa actttaaaca tctccctgag ccgttccgca ttcgtgttat tgagccagta
      361 aaacgtacca ctcgcgctta tcgtgaagag gcaattatta aatccggtat gaacccattc
      421 ctgctggata gcgaagatgt gtttatcgat ttactgaccg acagcggcac cggggcggtg
      481 acgcagagta tgcaggccgc gatgatgcgc ggcgacgaag cctacagcgg cagccgcagc
      541 tactatgcgt tagccgagtc agtgaaaaat atctttggtt atcaatacac cattccgact
      601 caccagggcc gtggcgcaga gcaaatttat attccggtac tgattaaaaa acgcgagcag
      661 gaaaaaggcc tggatcgcag caaaatggtg gcattctcta actatttctt tgataccacg
      721 cagggccata gccagattaa cggctgtacc gtgcgtaacg tctatatcaa agaagccttc
      781 gatactggcg tgcgttacga ctttaaaggt aactttgacc ttgagggatt agaacgcggt
      841 attgaagaag ttggcccgaa taacgtgccg tatatcgttg caaccatcac cagtaactct
      901 gcaggtggtc agccggtttc actggcaaac ttaaaagcga tgtacagcat cgcgaagaaa
      961 tacgatattc cggtggtaat ggactccgca cgctttgctg aaaacgccta tttcatcaag
     1021 cagcgtgaag cagaatacaa agactggacc atcgagcaga tcacccgcga aacctacaaa
     1081 tatgccgata tgctggcgat gtccgccaag aaagatgcga tggtaccgat gggcggcttg
     1141 ctgtgcatga aagacgacag cttctttgat gtgtacaccg agtgcagaac cctttgcgtg
     1201 gtgcaggaag ggttcccgac atatggcggc ctggaaggcg gcgcgatgga gcgtctggcg
     1261 gtaggtctgt atgacggcat gaatctcgac tggctggctt atcgtatcgc gcaggtacag
     1321 tatctggtcg atggtctgga agagattggc gttgtctgcc agcaggcggg cggtcacgcg
     1381 gcattcgttg atgccggtaa actgctgccg catatcccgg cagaccagtt cccggcacag
     1441 gcgctggcgt gcgagctgta taaagtcgcc ggtatccgtg cggtagaaat tggctctttc
     1501 ctgttaggcc gcgatccgaa aaccggtaaa caactgccat gcccggctga actgctgcgt
     1561 ttaaccattc cgcgcgcaac atatactcaa acacatatgg acttcattat tgaagccttt
     1621 aaacatgtga aagagaacgc ggcgaatatt aaaggattaa cctttaccta cgaaccaaaa
     1681 gtattgcgtc acttcaccgc aaaactgaaa gaagtttaat taatactaca gagtggctat
     1741 aaggatgtta gccactctct taccctacat cctcaataac aaaaatagcc ttcctctaaa
     1801 ggtggcatca tgactgatca agctgaaaaa aagcactctg cattttgggg tgttatggtt
     1861 atagcaggta cagtaattgg tggaggtatg tttgctttac ctgttgatct tgccggtgcc
     1921 tggtttttct ggggtgcctt tatccttatc attgcctggt tttcaatgct tcattccggg
     1981 ttattgttat tagaagcaaa tttaaattat cctgtcggct ccagttttaa caccatcacc
     2041 aaagatttaa tcggtaacac ctggaacatt atcagcggta ttaccgttgc cttcgttctc
     2101 tatatcctca cttatgccta tatctctgct aatggtgcga tcattagtga aacgatatca
     2161 atgaatttgg gctatcacgc taatccacgt attgtcggga tctgcacagc cattttcgtt
     2221 gccagcgtat tgtggataag ttcgttagcc gccagtcgca ttacctcatt gttcctcggg
     2281 ctgaagatta tctcctttgt gatcgtgttt ggttctttct tcttccaggt cgattactcc
     2341 atcctgcgcg atgccaccag caccactgcg ggaacgtctt acttcccgta tatctttatg
     2401 gctttgccgg tgtgtctggc gtcatttggt ttccacggca atattcccag cctgattatt
     2461 tgctatggaa aacgcaaaga taagttaatc aaaagcgtgg tatttggttc gctgctggcg
     2521 ctggtgattt atctcttctg gctctattgc acgatgggga atattccgcg cgaaagcttt
     2581 aaggcgataa tctcctcagg cggcaacgtt gattcgctgg tgaaatcgtt cctcggcacc
     2641 aaacagcacg gcattatcga gttttgcctg ctggtgttct ctaacttagc tgttgccagt
     2701 tcgttctttg gtgtcacgct ggggttgttc gattatctgg cggacctgtt taagattgat
     2761 aactcccacg gcgggcgttt caaaaccgtg ctgttaacct tcctgccacc tgcgttgttg
     2821 tatctgatct tcccgaacgg ctttatttac gggatcggcg gtgccgggct gtgcgccacc
     2881 atctgggcgg tcattattcc cgcagtgctt gcaatcaaag ctcgcaagaa gtttcccaat
     2941 cagatgttca cggtctgggg cggcaatctt attccggcga ttgtcattct ctttggtata
     3001 accgtgattt tgtgctggtt cggcaacgtc tttaacgtgt tacctaaatt tggctaaatc
     3061 cttcaagaag ccagccattc gctggcttct tgcctctcag gaaatcactt atgtccaaat
     3121 ggcaactcgc ctgattctcc ttcaccacgt atgctttgcg tcaccttact atcaggacgc
     3181 tttagcccat gtcccgcttt ttaatttgta gttttgccct ggttttactt tatcccgccg
     3241 ggattgatat gtacctcgtt ggtttaccgc gcatcgccgc cgatctcaat gccagcgaag
     3301 cgcagttgca tattgcgttc tccgtttatc tggcgggcat ggcagctgcg atgttatttg
     3361 ccggtaaagt ggccgatcgt tcagggagaa agcctgtcgc catccccggc gcggcgctat
     3421 ttattatcgc ttcggtgttc tgttcactgg ctgaaaccag cacgttattt cttgcagggc
     3481 gctttctaca ggggttgggc gcaggctgtt gttacgtggt ggcgttcgcc attttgcgcg
     3541 acacgctgga tgatcgacgt cgggctaaag tgctgtcgtt actcaacggt attacctgca
     3601 tcattccggt gttagcgccg gtgctcggac atctgattat gcttaaattc ccgtggcaga
     3661 gtctgttctg ggcgatggca atgatgggca tcgcggtact gatgttgtct ttgtttattt
     3721 taaaagaaac gcgccctgcg gcccccgcag cttcggacaa accacgagaa aatagcgagt
     3781 cgctgcttaa ccgttttttc ctcagccgtg ttgttatcac caccctcagc gtttcggtga
     3841 tcctcacttt cgtcaacacg tcaccggtat tgctgatgga aatcatgggg tttgagcgcg
     3901 gtgaatacgc caccattatg gcgctgaccg ctggcgtcag catgaccgtt tcattctcca
     3961 cgccatttgc gctgggaatt tttaagccac gtacgttgat gatcacctcg caggtgttat
     4021 tcctggcggc ggggatcacc cttgccgttt caccttccca tgcggtttct ctgtttggta
     4081 tcacactgat ttgcgccggt ttctcggtcg gttttggcgt agcgatgagt caggcattag
     4141 ggccgttttc attacgcgcg ggcgtagcca gctcgacctt aggtattgcg caggtttgcg
     4201 gttcgtcact gtggatttgg ctggcggcgg tggttggtat cggcgcatgg aatatgctga
     4261 tcgggattct gattgcctgt agcatagtga gcctgttgct gattatgttc gtcgcgcctg
     4321 gacgccccgt tgccgctcat gaagaaatcc atcaccacgc ttgatctcaa tttgctgctc
     4381 tgtctgcaac tgctgatgca ggagcgcagc gtgaccaaag cggcgaagcg gatgaacgta
     4441 accccttcgg cggtgagtaa gtcgctggca aagttaagag catggtttga cgacccgctc
     4501 tttgtgaact caccgctggg gctgtcgccc acaccgctaa tggtcagcat ggagcaaaat
     4561 ctggcggagt ggatgcaaat gagcaacctg ctgctggata aaccgcacca ccaaacgccg
     4621 cgcggcctga agtttgagct ggcggcggaa tcaccgctga tgatgatcat gcttaatgcg
     4681 ctgtcgaaac agatctacca acgttacccg caggcgacca tcaaattacg taactgggat
     4741 tacgattcct tagatgccat tactcgtggt gaagtggata ttggtttttc cggtcgcgaa
     4801 agccatcctc gctcgcggga gctgttaagc tcgctaccgt tagccattga ttatgaagtg
     4861 ctgtttagtg atgtgccctg cgtctggtta cgcaaagatc atccggcact gcatgaaacg
     4921 tggagtctgg acactttttt gcgttatccg catatcagca tttgctggga acagagcgat
     4981 acctgggcgc tggacaatgt gttacaggag ctggggcgcg aacgcacgat tgctatgagc
     5041 ctgccggaat tcgagcagtc actgtttatg gcagcgcagc ccgacaatct gctactggcg
     5101 accgcgccgc gctactgtca gtactacaat caactccacc aactgccgtt ggttgcgctt
     5161 cctctcccgt ttgacgaaag ccagcaaaaa aagctggaag tcccttttac cctgctttgg
     5221 cataaacgga acagccataa tccgaagatc gtctggttac gggaaaccat taaaaatctt
     5281 tacgcgtcga tggcataacc gaatcgtatg aaacgcgacc ctatttcaca ataaaatgta
     5341 aaaattttgt aataaacttg tctgaatcga acttttagcc gctttagtct gtccatcatt
     5401 ccagtaaatg attactcttg tattcataat ggaccattaa gcatggagcg aaaaatggcg
     5461 actcactttg cccgagggat tttaacggaa ggacatctga tttctgttcg tctcccctcc
     5521 cagtgtcatc aagaagcccg aaacattccc cttcatcgtc aaagccgttt tctggcgtcc
     5581 agaggtttac tcgcagaact gatgttcatg ctgtatggca ttggcgaatt gccggaaatt
     5641 gtcaccctgc cgaaaggtaa accggttttc agcgataaaa atttgccttc gttttctatt
     5701 tcctatgccg ggaatatggt tggcgtggcg ttaacaaccg aaggtgaatg tggcctcgat
     5761 atggaactac agcgtgcgac gcgcgggttt catagcccac acgcgcccga taaccacacc
     5821 ttttcaagca atgaatcgct atggatcagt aaacaaaacg atcctaacga agcgcgggcg
     5881 cagctcatca cgctgcgccg aagcgtgcta aaactaaccg gtgatgtttt gaatgacgat
     5941 ccgcgcgatc tgcagctgct gcccattgcc ggacgcctga aatgtgctca tgtaaatcat
     6001 gtagaagcgt tatgcgacgc ggaagacgtg ctggtgtggt ccgtggcggt cacgcccacg
     6061 attgaaaagc tcagtgtctg ggagttagat ggcaaacacg gctggaaaag cctgccggat
     6121 attcacagcc gcgccaacaa tcctaccagc cggatgatgc gttttgccca gctctctacc
     6181 gtgaaggctt tttcgccaaa ttgatagaca accacaggag tcatcatgtc tgaaaaattg
     6241 aaggtggtta cgttactggg gagcctgcgc aaaggctcat ttaatggcat ggttgcacgt
     6301 accctgccga aaattgctcc ggcgagcatg gaagtcaatg cgttaccttc cattgccgac
     6361 attcccttgt atgacgctga cgtacagcag gaagaaggtt ttccagcaac ggtagaagct
     6421 ctggcggaac agatccgtca ggctgacggt gtggtgattg tcacgccgga atataactac
     6481 tcggttccgg gtgggctgaa aaatgccatc gactggcttt cccgcctgcc ggatcaaccg
     6541 ctggccggta aaccggtgtt gattcagacc agctcaatgg gcgtgattgg cggcgcgcgc
     6601 tgtcagtatc acctgcgcca gattctggtt ttcctcgatg caatggtgat gaacaagccg
     6661 gaatttatgg gcggcgtgat tcagaacaaa gttgatccgc aaaccggaga agtgattgat
     6721 cagggtacgc tggaccacct gaccgggcaa ttgaccgcat ttggtgagtt tattcagcga
     6781 gttaagatct aaataaaaaa cccgccagta atcatgcatg gcgggttttt aatgcgctat
     6841 cgattttaat gagcgtcgat aaatacaatc ttcaggataa acatcagcgc aacgatgatt
     6901 acgcacgggc taaggtcacg cagacgcccg gtaccaatct tcatcacgca gtaggagata
     6961 aagcccagcg caataccttc ggtaatcgaa aagctgaacg gcatcatcac ggcggtaata
     7021 aacgccggaa cagattcagt aagatcctgc cagttcacgc gagcaagact tgaggtcatc
     7081 aacacgccaa cgtagatcaa cgcgccagct gcagcgtagc ctggcaccat tcccgccagc
     7141 ggcgacagaa agataaccag caggaacagc agaccaacaa ccactgccgt cagaccggta
     7201 cgaccgccaa ccgatacgcc ggaagaggac tcaatataag ccgtaacgga agaagtaccg
     7261 ataaacgaac cggtcacgga agagatactg tcgacataca gcgcctgctt catgcgcggg
     7321 aatttaccct tctcatccgc caggcctgct ttatcggtca cgccaatcag cgtaccggag
     7381 gagtcaaaca ggttgaccag catgaaagag aaaatcaccc ctgccagccc gaggttaaac
     7441 gacccggcta aatctacatg gcccacgaca gtcattacgc tcggcggcgc agaaacgatg
     7501 ccattgtagt ggacatcacc cagcatccag cccagcagcg ttgtcaccac gatggaaacc
     7561 agcaccgccg cgtgaatgtt gcgcgaggcc agaatagcga tgatgaagaa gccgaggatc
     7621 cccagcagca cgctgtgaga agtcaggtta ccgatgctga ccagcgtttc cgggttagcg
     7681 acaatcacac ctgcattctt cagtcccatc ataccaatga acagaccgat accgctggtg
     7741 atgcccacac gcagacttac cggaatgtta gcaatcatcc agtaacgaac gcggaaaatc
     7801 gtcagtaaca gcagaccaat cgcgccccag aagattgcgc ccatcccgac ttgccacggc
     7861 aagcccatag cctgtacaac gacaaaagcg aagaatgcat tcaggcccat agcgggtgcc
     7921 agtgcaactg gcaggttagc aaacagtccc atcataatac tgccgaatgc agcaatcaga
     7981 caggtagtaa cgaagacggc gctggtatcc atgccagcaa cgccaagaat ttgcgggtta
     8041 acaaaaacga tgtaaaccat cgtcaggaag gtggtaaaac cggcgatcac ttcggtccgt
     8101 gccgtcgtgc catgttcgcg cagtttaaac acgcgttcca gcatcccctg gccagaagcc
     8161 tgggttgtgt gttgttgact cattatctat ttccgaacaa ggagggaaaa tccgtcgcta
     8221 tcgtatacca aaatgagaca atatggacgg ctgaagataa ctttttttca ttgtatttgc
     8281 ttatacggca acgattgcgt catgcgaatt ggcatgacga aaacgttaaa ctaacgataa
     8341 aggaaagaca tgtcccagat agaagcggta tttttcgact gcgacggtac gctggtcgac
     8401 agtgaagtca tttgctcccg cgcatatgta acgatgtttc aggaatttgg tattacgctc
     8461 gatcctgaag aggtattcaa acgtttcaaa ggtgtaaaac tgtacgaaat tatcgatatt
     8521 gtttcccttg aacatggtgt tacgttagcg aaaacagaag ctgaacacgt ttaccgtgca
     8581 gaagtcgctc ggctgttcga ttcagaactg gaagccatcg aaggggctgg agcgctcctg
     8641 tcagcgatca ctgcgccaat gtgtgtgtat ctaacggccc aaataacaaa atgcagcatt
     8701 ctatgggcaa gctgaatatg ttgcactact tcccggataa actgttcagc ggctacgata
     8761 ttcagcgctg gaagccagac ccggcgttaa tgttccatgc ggcaaaaacg atgaatgtaa
     8821 atgtagaaaa ctgcattctg gttgatgact cagttgccgg tgcgcaatct ggtatcgacg
     8881 caggtatgga agtgttctac ttctgcgccg acccgcacaa taagccgatc gttcacccga
     8941 aagtcaccac ctttacccat ctttcgcagt taccggaact gtggaaagcg cgtggttggg
     9001 atattacggc atagttcttc acactccctt cacttacccc gcttaaattg gcgctcaaag
     9061 gtaagtaaag ggagtttgat atgtctgttt cacgtcgggt aatacatcac ggactttatt
     9121 ttgcggtttt aggaccgtta attggtgttc tgtttcttgt cctctacata ttcttcgcaa
     9181 aagaaccgct ggttcttttg cgaagaatac aggtacttcc attgtttctc ttattgtcga
     9241 ttactacggg agttattcct gcattgttaa ccggcgtgat ggtcgcctgt ctgccggaaa
     9301 agatcgggtc acagaaaaat tatcgttgtc tggctggtgg cataggtggc gtcgttatca
     9361 ccgagatcta ttgtgcagtt attgtacata ttaagggcat ggcttcctcg gagttgtatg
     9421 aaaacattct ttctggtgac agtctcgttg tccgcatcat tcctgcattg ctggcaggtg
     9481 tggtgatgag cagaatcatt acccgtctgc ccggattgga tatttcatgt cctgaaacag
     9541 actctttaag ttaagcggga tactttatct ttgggctact caaaagcaga caggatgtta
     9601 ctatgactca aaatatcagg ccgttacccc aattcaaata tcatcccaag ccactggaaa
     9661 caggcgcatt tgaacaggat aaaaccgtag agtgcgattg ctgtgaacaa cagacgtcag
     9721 tttattactc gggtcccttt tattgcgttg atgaagttga acatctctgt ccgtggtgta
     9781 ttgcggacgg ttctgctgct gaaaagtttg caggtagttt tcaggatgat gccagcatag
     9841 aaggtgttga atttgagtat gatgaagagg acgaatttgc cggtattaag aacacatatc
     9901 ctgatgaaat gctgaaagag ttggttgaac gcacgccagg ttatcatgga tggcaacagg
     9961 aattctggct tgcgcattgt ggcgatttct gtgcttttat cggctatgtg ggctggaatg
    10021 atataaaaga tcgcctcgat gaatttgcca accttgaaga agattgtgag aatttcggta
    10081 ttagaaattc tgatctagct aaatgcctgc aaaagcgtgg tgattgtcag ggttatctct
    10141 tccgctgtct ccactgcggc aagctgagac tgtggggtga tttttcgtag ttatttaaat
    10201 aatgagaaca ggccggagcg taattcatac atccggcctt atttcttcag cttaaccgag
    10261 cgctaattct gcggcagcgg ctttatcagc gattaccatc agcgaaggat gcagctgcaa
    10321 cacggaagct ggaacatctt cggtaaccgg accttgcagc acatttttca gtgcctgcgc
    10381 tttacctgcg ccactgacaa ttatcagcaa gtttttcgcc gccatgatgc ttttcggccc
    10441 catcgtcacg tagctgtcag gcactaacga aaagtcgccg cccaactcac catgcgccac
    10501 aatatcaacc atctctccct ggatcgggaa ctccacggtt tgctcatgga aatgggtcgt
    10561 attcggcaaa ttgccacaaa aatgaccatc tgcacctaat cccagcacca ccaaatccag
    10621 gccgccttca cgcgccagtt tctgatcatg ctcgcggtag ttatcaatag tgagcttctg
    10681 gatattctct tctttgatcc ccgcaggggt gaaaaacaga ttacgcagat tggtaatcgt
    10741 tacaccttct ccctctttgc cgcgaaaggg aatttcatcg aaattataga aatagcagtt
    10801 atcataccag ggcttacctt taaccagggt ggtgaggtat tcatacatgc ctttgggcgt
    10861 gctaccggcg gtaattgcca ggttaacacg acgcgtcttc gacatataac caagcagatg
    10921 gtgtgccgcg acacggctca tttcctggta atcttcggta atgattaatt tcatcttagt
    10981 gccttatttt atttaaataa tttctgtacg aaggctgcat aggcaggccg ccagacatcc
    11041 atttcatggt tcaggccggg atattcctgg taatcaaact taattttttt ctgctcaagc
    11101 tcagttttca gcccggcgat atccttgccg gttacgacat ctttatcacc aacgaccaca
    11161 gtaaaattac gtagttgctg gttaatagct gccggatcgt tcagccgggc cgcgacacct
    11221 tcatccggta cggttgtcgt ggtaacacca ctgaatgtgg ccagccagcc aaagctttcc
    11281 agatgattca ttccggaaac cagcgcctgg tacccgcctt gtgaaagccc tgccagcgcg
    11341 cgaccatcgg catctttacg gacattaaaa cgcttgctaa tcagcgggat aatatcgttc
    11401 atcagttcgc gatctgccgc tttagcattc agcggataaa agactttacg tctttcctga
    11461 ggcacgaaat cttcgggaat aatgcccttc gcatcggttt cggtatccgg gatcaccacc
    11521 agcatcggtt taattttccc ttcagccagc aggttatcca tgatttgcgg gatacgcccc
    11581 tgatcgatag cggaacgtcc ggtatcacca aaaccgtgat agaaatagag cactggcaaa
    11641 ggctcgccca tgccggtgta tcctggcggg gtccagacat acatctgacg ttcagattgc
    11701 aatgcgttgg agtggtaagt tatggcgatc aaatcaccat gcgcaacaga acgcgtatcc
    11761 agataactgc ctggcaccag aatcatactg gagttaacct ggcgctgagg cttagtcatt
    11821 gcggtgcctg tatcaatgct gcgtacacca tcaacattga aaaaatactc gtacaggttg
    11881 cctttcagga taggtgtgcg ccacgaccag actcctgctt cgtctttggt catcgggtga
    11941 atattgtccg gaaccggaac acccaccact acagagacat tttttgcccc aggggcaaag
    12001 tagcgaaagg tcacgctgtt atcggcattg acctgagtca catactgttt caccgggata
    12061 gtgggtgccg ggctggctgg catatccgct gctatggccc actgagcaga aataccgctg
    12121 gcaatagcca gcgttaaagc agcaattttt atattcatcg taatattcct ttcattaagt
    12181 tccattacca ccagatttca gcctgggcac cgacagcgaa ctggtcgtct ttattgtctt
    12241 cgaaggtgaa gccatccagt tcgttttcca gggctttgat atacgtggcg tagaaacgaa
    12301 tttccggacg cgaggtcaac atactggtat tgactttaaa ggtatggaag agcgtggttt
    12361 tataaccaga ctcattaaat ttattactat tcgcatcttt gttttgctgg gtgaaatagc
    12421 ccagttcaac acctgtctgg ttatattggt cccaaatata ggccgggcga acaaccgcac
    12481 gaatagattc gaaatcagag tgtgcgcctg tttcgtagct ataaatatcg ttaccgaagg
    12541 agtaaacaat cgcgttagcc acaataaaat gatcgccgat ataggcttcg ccctgtgaag
    12601 tcagacgaac tgctgttccg ccggtgtgat caccataata acgaccatta aaggtggtaa
    12661 atggacttgc gccagcataa cggccaaagt tactggcgat agagttattc gcaaccagga
    12721 aggagaattc gttgaagcca cctttatcaa atttctgcgt taaagatgtg ccgaacatcc
    12781 aggtatcttt ccagtcataa tacccgttat tatcctgatt atctttttcc gatgcgcttt
    12841 cgtttgccgt gacataacga ccactcacca ttaaggtcgc tttatcccat aacgggatat
    12901 ctttatagcg taaatcaata gtattggtat taatctgctg tttgttttgc aggctgcgat
    12961 cgtaatcatc gacatcttcg cgaaccagcg cgatatcaat tttacccgga ccgactttcc
    13021 agttttccag acctacaccc gctgcggcat cagtacgctg cgttttccag tccagcatct
    13081 ggatttcaat tttcggcgca ccgtgtttac ccacccagaa atcagcctct ggcgcaaagg
    13141 gcaggaaacc tttggtggta acgtacatat cggagaactg cataaagttc tcgccaccgg
    13201 cgttatcgcc aaaccagccg gtagagtact gctgaccaac gttaccatcc atcatcacaa
    13261 cggcatcaac ccgtttgccg ttttcgttgt agacacgttg ttttaactgc aaatcaaacc
    13321 agccggagta ttcgttacca aagcggccca gagaaccaat cgcccatgat ttaggtgaac
    13381 catgagaggc ggtcccccaa ccagaacggt aatagccgtt gtaactaaag ccaatttcat
    13441 ctttcacaaa cttactgaaa tctttcagag tcatggaaga ataaatcgag cctgtctcac
    13501 tggcattttt ctcttcattt ttgaccagta cagcatcagg ttttgcggca ctactggtcg
    13561 ggaacggatt ggcggcatac ccttgatcat tcgtacttac gctacgattc accgtcgctg
    13621 gcgtatattt tttcttctct tcatctttat actttttgag ttcgctttgc gtttctctta
    13681 acgctttttc taataactca aggcgttgtt ccaccgttaa tgattcagcc aatgattgtg
    13741 cagaaaaggc taacggtgcc attaataaga cggcagaggt aataagattt cgtctaaaca
    13801 taatatccct ttatggtgca aagaaagaat taacgtatcg catccagact attctgaatg
    13861 cgacgataat taaggtgctt tattggttat ttttttaatg acagcccccg cgttttgatc
    13921 acttctgcat accatccgaa gcttttctta cgcgttcttg tgaggcttcc ttcgccatta
    13981 tcatcacgat ccacataaat aaagccgtag cgcttagaca tttgtgaatg agatgcactg
    14041 actaaatcaa ttggccccca actggtgtac cccataatat ccacaccatc ggcaatcgct
    14101 tcatttacct gtaccaggtg atcgtttaaa taggcaattc gataatcgtc ctgtatcgaa
    14161 ccatccgctt caacgctgtc ttttgcgcct aatccgttct cgacaataaa taacggtttt
    14221 tgataacgat cccaaagcgt atttaacaga acccgtaatc caaccggatc aatttgccac
    14281 ccccactctg aacttttcag atgcggattg gggatcatat tcagtatgtt gccctgcgca
    14341 tttttattaa tgctttcgtc gtgggaaaca caaccagtca tgtaataact aaaagagatg
    14401 aaatcgacgg tatgttttaa atcttctgcg tcactttcag tcatctcaat ggtgatattg
    14461 tggtcgcgga agaaacgctg catatagccg ggatactggc cacgcgcctg aacatcacca
    14521 aagaacatcc agcgccggtt ctcttccatg gcctgcaaca tatcctgtgg ctggcaggtg
    14581 agggggtaaa ccagcccacc gagaagcata ttgccgattt tcgcttcggg gagcaggcta
    14641 tgacaggctt taactgcccg cgcactggca accagttgat ggtggatagc ctgataaact
    14701 tccgcctcgc cactctcttc tgccagcccc acgcccgtga atggcgcgtg taacgacatg
    14761 ttgatttcat taaacgtcag ccataacgcc actttatgtt ggtagcgagt aaagaccgtg
    14821 cgggcgtaat gctcgaagtg atcgatgacc gctcgattag cccaaccgcc gtagtttttc
    14881 accagcccat atggcatttc gtaatgggat aacgttacca gcggcttgat ccccgcctgc
    14941 gccatttcat caaacagccg atcgtaaaac gctaaccccg cttcattcgg ttcgacttcg
    15001 tcgccctgag ggaaaattcg cgcccaggca atggaaatac gcagacaggt gaagcccatc
    15061 tcggcaaata acgcgatatc ttccgggtaa cggtgataaa aatcgatggc gacatctttg
    15121 atattctctt tccccaggat gcgcggttcc atttttccca ttacgccatg aggctgtaaa
    15181 tctgaggtcg agatcccttt gccatcttcc tgccaggcac cttccacctg attggcagct
    15241 gttgcgccac cccaaagaaa tgtttctgga aatgctttca taattaactc cttttatcgt
    15301 tagcgaatga tggataacag cggttcacct gcgcttatct gcgccgtgcc gtggggtaat
    15361 acgtccgtaa aatcatcgct attactgatt aataccggcg tcgtcagatc aaatccggcc
    15421 tcgcgaatag cagggatatc aaaagaaatc agccgatcgc ctgtattgac cttgtcaccc
    15481 acgttgacgt gagcggaaaa gaatttgccg tccagtttta cggtgtcgat accgacatga
    15541 atcaggatct ccacaccatc atctgactca atgccaatgg cgtgtaatgt ggcgaacaac
    15601 gaagcaattc gacccgcaac cggagaacgc acttcaccaa ccgagggcag aatggcgata
    15661 cctttaccca acaggccact ggcaaacgtg gtatcagcga cgtgaatgag cggcacaatc
    15721 tctcccgtca tcggtgaaca gataccgccc tgctcaggtg gtgtaataac ctctggtgtt
    15781 ttctcttgcg gggcaccctg cgctggctga cgtttagcgg tgatgaaatg aagcatcacc
    15841 gtaccgacaa atgcgcaacc gatggcaatg acaccgccaa taacgctggc ccagacggtg
    15901 aaatcaattc ccgttgacgg gatggtttgc atgaaggtga aaatacttgg caaaccaaag
    15961 gagtagactt tcgtttgcgc gtagccaatg atggtggccc ccaaagcccc actgatacag
    16021 gcgataacaa aggggtactt acgcggcagg ttgacgccat ataccgctgg ttcggtgata
    16081 ccaaacagac tcgtcaacgc cgctgatccc gccaccactt ttttctgcgc atcgcgttcg
    16141 cagaggaaga cgccgagcgc cgccccgacc tgcgccataa tggcgggcat taacagcggg
    16201 atcatggtgt cgtagcccag cacggtgaag ttattgatac acagcggcac caggccccag
    16261 tgcagtccga acatgacgaa gatttgccag aagccgccca ttaccgcgcc cgcaaatgca
    16321 ggaaccgcct gataaagcca gagataaccg gcggcaatca gttcgcttat ccaggttgat
    16381 agcggcccca ccagcagaaa ggtgacgggt gtgataacca tcagacatag caatggtgtg
    16441 aagaaatttt tgattgccga cggtaaccac gcattaagtc ggcgttccag aatgctgcac
    16501 aaccaggcag aaaaaataat gggaataacc gatgacgagt aattcaacaa tgtgaccgga
    16561 atacccagga aatccagccc cagcgcatcc gctttttgcc cgttctcgaa agcagtcaga
    16621 attaatggat gcactaacgc tccaccaatc accatggcag taaatggatt accgccgaag
    16681 cgtttccccg cggtgtatcc caggattatc gggaagaacc aaaacaaggc atcactggcg
    16741 ctgaataaaa ttaaataagt accactttgt tcggtcgtcc actgaaaagt gagcgccaga
    16801 gccagcatac ctttcaggat cccggttgcc gccatcaaac cgatcagagg cgtaaaaata
    16861 cctgaaataa cataaacaaa gcggtttagc agattacctt tatcatcatt ttccggtgcc
    16921 tgttgcgctt tttcgtcaag gcctgccaca ctgttaaccg ccaggaagac atcggccaca
    16981 tggttaccta tgaccacctg aaactggcca ccgctttcca ccaccataat aataccgggg
    17041 gtctttttca gtacctctgc ttgcgctttg ctttcatcct ttaacttaaa acgtaatcgc
    17101 gttgcgcaat gcatcagact cacaatgtta tctgcgcccc cgactcctgc gactattttt
    17161 ctggctaact ccgtcataac ttgccctcta ccgctttgcg gcaaaactca aaaaaaaaac
    17221 ctgaaaaaaa cggcctgacg tgaatcaagc aattttttca ggttttgccc gcttagtgcg
    17281 gtaacaatcc tttactcagt aataatattt cagtgttctt tgtgcacgcg ctctatattt
    17341 atggctaaaa acataatctc tgcgggtgag attttacgtt gatactgcaa accaataaaa
    17401 atggcgatcc gctccgcaca ttgccatgct tgcgggtaat tttgttttac tgcttgttgt
    17461 aatgactcat cactatcgtt aattgaagca tgtccaagaa tacgccagga taaaaacttc
    17521 agatgcgtaa ccagtcgctg ataactcaag ctttcttcct ggtaattaag gctgaactga
    17581 aattttatta attgcagcat ttcgcgcatt aattgcgtga cacctgcaac atcctccata
    17641 tttccgctca tttgggcact gaccagatgc atggcaataa agcccacttc atctttcggt
    17701 aactgcacgc ccaaccgttt atcaataatg gttaacgctt cttcccctag ctggaactct
    17761 ttcgggtaaa gccgctggat atcccacagc agcgggttgg gcagtaacac gttttgctga
    17821 aagcgtttaa tcgcaaactg gcaatggtca gttagcgaga tataaatact gtcctgtaat
    17881 tttcccaagc gctcctgcgc taaagagata atacgatcac aggttgccat cacctcaaga
    17941 ggaatatgac ttaagagttc gcttaatcgc ccgttcagtt catgactgct caaggcatac
    18001 tctttttcta ttccacttga gttaattctt tcgccagcgc gtttttgaaa gccaattcct
    18061 cgccccatga cgactttttc ccgctgttga tcatcaataa ccaccacaac attattgttg
    18121 agaattttgg tgatttgcat gttcatagca aggacctttt ttataaacaa aaaaacccga
    18181 cttcaccagt attctctggt tatgtcaggt tttgcctgcg aatgcagtaa caatccagtc
    18241 atttattaat ggtttttata acgaacatcc aggttcggaa attaatttaa ttgcgtgcag
    18301 agaaaatagc aatgcgctat tgataaaaat atgaccatgc tcgcagttat taactttgtg
    18361 taattttagg aatttataaa gttatatata acaaatccca ataattaagt tattgggatt
    18421 tgtctggtga attatttgtc gctatctttt cccgccagca gtttatccag ctcatcaccg
    18481 cctacatgac ggaaatcctg ccccttcacg tagtagaaga taaactcaca aatattctgg
    18541 cagcggtcgc caatacgttc gatagaacgc gcgcagaaca gcgcagtaag tacgctcgga
    18601 atggtacgcg aatcttccat catgtaggtc atcagttgac gaacaatacc ttcgtattcc
    18661 tgatcgactt ttttatcttc acgataaata cgtaccgctt cgtcaatgtc catccgcgcg
    18721 aacgcgtcca gcacgtcgtg cagcatctgg atggtatgac ggcccagcga ctccagactt
    18781 accagcaacg gctgatgctg ctgggagaat ttctccagcg cagtacggca gattttgtcc
    18841 gccacgtcgc caatacgctc cagctcggca atggttttac tgatcaccat aaccagtcgc
    18901 aggtcgctcg ccgtcggctg acgtttggcg ataatgcgca cgcaggcttc atcgatcgcc
    18961 acttccatca tgttgacgtt cttgtcgcct tcgatcacgc gcttcgccag atcgctgtcc
    19021 tggttatgca tcgcggtgat tgcatcagaa agctgctgct ccaccatgcc gcccatggtc
    19081 atcacctgcg tgcggatact ttccagttcg gcgttgaact ggccggaaat atgtttatta
    19141 agattgagac tgtccataac gcactcctga atcaaccgta acgaccggtg atgtagtctt
    19201 ctgtttgttt cttcgctggc ttggtgaaca gatcgtccgt gttgctgaac tcaatcaatt
    19261 cgcccaggta cataaacgcc gtgtggtcgg aacaacgcgc agcctgctgc atgttgtggg
    19321 tgacgatcac cacggtgtaa tcctgcttca gttcggtgat cagctcttca atacgcccgg
    19381 tagagatagg gtcgagcgcc gaacacggtt cgtcgagtag cagcacttcc gggcgaatgg
    19441 cgataccacg cgcaatacac agacgctgtt gctgaccacc agagagagag taaccgctct
    19501 ggtgcaattt atctttggtt tcgttccaca atgcggcttt ggtcaatgcc cactgcacgc
    19561 gctcgtccat gtcggcacgg gagagcttct caaacagacg aacgccaaaa gcgatgttgt
    19621 cgtagatgga catcggaaac ggcgtcggtt tctggaacac catgcccact ttcgcacgca
    19681 gcagggcgat atcctgagag ttggtcagga tgttgtcgcc atcaagcaga atttcacctt
    19741 ctgcgcgctg ctccggatac agttcaaaca ttttgttgaa ggtacgcagc agcgtcgatt
    19801 taccgcagcc ggacggcccg ataaacgccg ttacctggtt tttagcgata tccaggttga
    19861 tgtttttcag ggcatggaat ttgccgtagt agaagttcaa attacgaacc tgaattttac
    19921 tcggggcagt ttcaaccata ctcattgcaa tcttttcctc atcgtacctg atgcttttcg
    19981 ctaatcaggc ttaccgtagg ccgggtaagg cgttcacgcc gcatccggca aagtgttaac
    20041 cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt cagcagcagg acgcacaggg
    20101 taatgatcaa taccccggcc caggccagtt gttgccattc cgcaaacggg ctcatcgcaa
    20161 acttaaagat cgtcaccggc aggttggcga tcggctgcat catgtccgtg ctccagaact
    20221 ggttggagag cgcggtaaac agcagcggcg cggtttcacc ggcaatacgg gcaatcgcca
    20281 gcaggatacc ggtcataatc ccggacaccg acgctttcag cgtaatcgcg gagatcatct
    20341 tccacttcgg tgtacccagc gcataagccg cttcacgcag gctgtccggc accagtttca
    20401 gcatgttctc ggtggtgcgg ataacgatcg gcacctgcaa cagcgccagc gcaattacgc
    20461 ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat ggtgtagaca aacagaccaa
    20521 ccacaatcga cggcgcggag agcagaatgt cgttaatgaa gcgaatcact tctgccagcc
    20581 aggatttacg accatattcc gccagataaa tccccgccat aatgcccagc ggcgtaccga
    20641 ataccgtggc ccacaaaatt aacagcccgc tacccgccag ggcgttcgcc agaccaccac
    20701 cttccgtatt gggcggcggc gtcatttcag tgaacagcgc cagcgacata ccgtcgatac
    20761 cgcgagtgat ggtggacatt aaaatccaga tcagccagaa caggccgaag gccatcgtcg
    20821 ccatcgagag cgtcagcgca atacggtttt tgaggcggcg acgcgcctgc attttgcggc
    20881 gagattcagc cagcgccgca gtggtttgca tttcaaccat agccattagc gtgccccctc
    20941 attcttagcc aggcgcataa tcataaactt cgatgcggcg aggacgatga aggtaatcac
    21001 aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc agaccggatt ccgcttccgc
    21061 aaattcgttc gccagcgcag aggtgatact gttgcccggc atatacagcg aggcgctgtc
    21121 gagctggtag gtgttaccga tgataaaggt caccgccatg gtttcaccga gcgcgcggcc
    21181 cagccccagc atgatgccgc cgataacacc atttttggtg aacggaagaa cgatacgcca
    21241 gataacttcc caggtggtgc agccaatacc gtaggccgac tctttcatca tcaccggggt
    21301 ttgttcgaac acatcacgca ttaccgccgc aatgtacgga ataatcatga tggcgaggat
    21361 cacgcctgcc gcgaggatac cgataccaaa tgcggggcca gagaacagcg cgccaacaat
    21421 cgggatattc gacatgatat tgccgaccgg ctcctgaaag taaacggcga acagcggcgc
    21481 aaagataaac aggccccaca tgccgtaaac gatacttgga atggctgcca gcagctcaat
    21541 ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc tcagtcagga acagggcgat
    21601 accgaaactc accgggacgg cgatcagcag cgcgataaac gaagtcacca acgtaccgta
    21661 gatcggcacc agcgccccgt agatatcgtt cggtgcatcc cactctttgg tccataggaa
    21721 agccagacca aatttctgaa tgctcggcca ggaggagatg atcagagaga caataatgcc
    21781 acccaacatc aatagcacaa tcagcgccgc cagttttacc agcacgctga aaattatgtc
    21841 gcccttttta cccggtgggt taaaagcagg cttggttgca gccataagtt actcttcagt
    21901 taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg cgtaaaacac cgtacccggc
    21961 ctggagtttt attagtacag cggcttaccg ctactgtctt taatattggt cttccacgca
    22021 gcgcgaacct gttcaactac actatccggc aggctggcgt aatccaggtc gttcgcctgt
    22081 ttagccccgg ttttgtacgc ccagtcgaag aatttcagca cttctgtgcc ttgttctggt
    22141 ttcttctgat ctttgtggat cagaatgaac gtggtagagg taataggcca tgcatcttcg
    22201 cctttctggt tggtcagatc ctgagcgaag gttttgctcc agtctgcacc ttttgctgca
    22261 ttagcgaagt tttcttcggt cggactaacc ggtttaccat cagcggagat cagtttggtg
    22321 tacgccaggt tgttctgctt cgcgtaagca tattcaacat aaccaattgc acccggcaga
    22381 cgctgaacga acgcggcgat accgtcgtta cctttaccgc ccagaccgat cggccatttt
    22441 acggtagagc cagtaccaac gttgtttttc cactcttcgt tcactttcgc caggtagctg
    22501 gtgaagacga aggaagtccc ggagccatct gcgcggcgta ctacagcaat gttttgtgaa
    22561 ggcagtttca gacccggatt cagtttggcg atggcttcat catcccactt cttgattttg
    22621 cccaggtaga tgtcgccgag ggttttacca tccagcacca gttcgccaga cttcagccct
    22681 ggaatgttaa ccgccagcac cacgccgcca atcacggtcg ggaactggaa cagaccttcc
    22741 tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac caaaatcaac ggtattagcg
    22801 ataatctgtt ttacgccacc ggaagaaccg ataccctggt agttaacttt attaccggtt
    22861 tctttctggt aagtgtcagc ccatttggca tacaccggcg cagggaaggt tgcacctgca
    22921 cctgtcaggc ttgcttctgc aaacacagag aaagcgctca tcgataaggt cgcggcgaca
    22981 acagttgcga cggtggtacg cataactttc ataatgtctc ctgggaggat tcataaagca
    23041 ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt gacagttata tgtaaggaat
    23101 atgacatttt tatgacagaa aggaattacc aaataactta ctgattttaa atgtgaagat
    23161 gaaaatccca catgtcatct ttttgtcatg atgcatttaa aagtgataaa gaaatgggtg
    23221 agatgacaac gatgtgatga acaatcgttg tcagacctat tagcgcacaa tctcaacagt
    23281 tatcgtcgct gttgcagtaa attgccccgg ggctgggcga gctccagtaa ggctcgcagg
    23341 agcagctgaa aaagttacac tacctgattt ttcaccatac actgttgatt tacccatgtc
    23401 tgttggcaac tcaccaccgt tcactttgat ttccttatta tttttgtcgt aaatttttat
    23461 ccccacgtta gagtttgaag ttacgaccat tgaattgtcc gtattgggtt cggtagcata
    23521 aaaagacgta ctgatatcat cggtattcag tatccctagg ttttcacact caatagtgac
    23581 cgttttagta attttatgtg ttgttaccgc acttccaaca acagatgaaa aatctgcagc
    23641 tggtatttct ttaaaatcaa aataaactgg ctgtaaatta tttatttcgc aactttgcgg
    23701 agctgtaata tcgccccgaa tataaacatc agccatcggc tgtgagtaat ccaccggggt
    23761 gtcacggcta attgttccat acaatgtcac tatctttgtt tcaggaataa tcaattttcc
    23821 t
//
LOCUS       DBANUU010000074        23474 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000074 DBANUU010000000
VERSION     DBANUU010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.074"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(9..329)
                     /gene="chbB"
                     /locus_tag="ACIE68_002803"
     CDS             complement(9..329)
                     /gene="chbB"
                     /locus_tag="ACIE68_002803"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFX5909091.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(628..966)
                     /gene="osmE"
                     /locus_tag="ACIE68_002804"
     CDS             complement(628..966)
                     /gene="osmE"
                     /locus_tag="ACIE68_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFX5909092.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1168..1995
                     /gene="nadE"
                     /locus_tag="ACIE68_002805"
     CDS             1168..1995
                     /gene="nadE"
                     /locus_tag="ACIE68_002805"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFX5909093.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLRTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2225..3112
                     /gene="cho"
                     /locus_tag="ACIE68_002806"
     CDS             2225..3112
                     /gene="cho"
                     /locus_tag="ACIE68_002806"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFX5909094.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3072..3647)
                     /gene="ves"
                     /locus_tag="ACIE68_002807"
     CDS             complement(3072..3647)
                     /gene="ves"
                     /locus_tag="ACIE68_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFX5909095.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3850..4335)
                     /gene="spy"
                     /locus_tag="ACIE68_002808"
     CDS             complement(3850..4335)
                     /gene="spy"
                     /locus_tag="ACIE68_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFX5909096.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4665..5633)
                     /gene="astE"
                     /locus_tag="ACIE68_002809"
     CDS             complement(4665..5633)
                     /gene="astE"
                     /locus_tag="ACIE68_002809"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFX5909097.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5626..6969)
                     /gene="astB"
                     /locus_tag="ACIE68_002810"
     CDS             complement(5626..6969)
                     /gene="astB"
                     /locus_tag="ACIE68_002810"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFX5909098.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6966..8444)
                     /gene="astD"
                     /locus_tag="ACIE68_002811"
     CDS             complement(6966..8444)
                     /gene="astD"
                     /locus_tag="ACIE68_002811"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFX5909099.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHARTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANAGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8441..9475)
                     /gene="astA"
                     /locus_tag="ACIE68_002812"
     CDS             complement(8441..9475)
                     /gene="astA"
                     /locus_tag="ACIE68_002812"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFX5909100.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9472..10692)
                     /gene="astC"
                     /locus_tag="ACIE68_002813"
     CDS             complement(9472..10692)
                     /gene="astC"
                     /locus_tag="ACIE68_002813"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFX5909101.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTSNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            11138..11944
                     /gene="xthA"
                     /locus_tag="ACIE68_002814"
     CDS             11138..11944
                     /gene="xthA"
                     /locus_tag="ACIE68_002814"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFX5909102.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12111..12821
                     /gene="ydjX"
                     /locus_tag="ACIE68_002815"
     CDS             12111..12821
                     /gene="ydjX"
                     /locus_tag="ACIE68_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFX5909103.1"
                     /translation="MNAERKFLLACLIFALVIYAIHTFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            12826..13503
                     /gene="ydjY"
                     /locus_tag="ACIE68_002816"
     CDS             12826..13503
                     /gene="ydjY"
                     /locus_tag="ACIE68_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFX5909104.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            13518..14225
                     /gene="ydjZ"
                     /locus_tag="ACIE68_002817"
     CDS             13518..14225
                     /gene="ydjZ"
                     /locus_tag="ACIE68_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFX5909105.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            14225..14773
                     /gene="ynjA"
                     /locus_tag="ACIE68_002818"
     CDS             14225..14773
                     /gene="ynjA"
                     /locus_tag="ACIE68_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFX5909106.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            14783..15949
                     /gene="ynjB"
                     /locus_tag="ACIE68_002819"
     CDS             14783..15949
                     /gene="ynjB"
                     /locus_tag="ACIE68_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFX5909107.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEAKLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            15922..17457
                     /gene="ynjC"
                     /locus_tag="ACIE68_002820"
     CDS             15922..17457
                     /gene="ynjC"
                     /locus_tag="ACIE68_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFX5909108.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            17457..18110
                     /gene="ynjD"
                     /locus_tag="ACIE68_002821"
     CDS             17457..18110
                     /gene="ynjD"
                     /locus_tag="ACIE68_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFX5909109.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            18177..19484
                     /gene="ynjE"
                     /locus_tag="ACIE68_002822"
     CDS             18177..19484
                     /gene="ynjE"
                     /locus_tag="ACIE68_002822"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFX5909110.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(19493..20113)
                     /gene="ynjF"
                     /locus_tag="ACIE68_002823"
     CDS             complement(19493..20113)
                     /gene="ynjF"
                     /locus_tag="ACIE68_002823"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFX5909111.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            20200..20607
                     /gene="nudG"
                     /locus_tag="ACIE68_002824"
     CDS             20200..20607
                     /gene="nudG"
                     /locus_tag="ACIE68_002824"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFX5909112.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLESFMALRAARPAD"
     gene            complement(20573..20845)
                     /gene="ynjH"
                     /locus_tag="ACIE68_002825"
     CDS             complement(20573..20845)
                     /gene="ynjH"
                     /locus_tag="ACIE68_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFX5909113.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            21081..22424
                     /gene="gdhA"
                     /locus_tag="ACIE68_002826"
     CDS             21081..22424
                     /gene="gdhA"
                     /locus_tag="ACIE68_002826"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFX5909114.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLERQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(22541..>23474)
                     /locus_tag="ACIE68_002827"
     CDS             complement(22541..>23474)
                     /locus_tag="ACIE68_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFX5909115.1"
                     /translation="MLLCKWGGYLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLL
                     IMMGLIVRAGFKAKKEQLHYYQAKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWP
                     RKPEPGKDTFQYHVLPFDSIDIISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANT
                     FKANMEEAPEQVIALLNKFAVFPSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTA
                     FQNGTIEEELAWHYIMLASRKAHELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELE
                     ACYRYDKQFWEHYSKKCRWPIRNVPWGASSVKYS"
BASE COUNT         5611 a         6221 c         5972 g         5670 t
ORIGIN      
        1 aaaataaatt aatttgctgc ggctttttta atcgctgcaa ccgcagcctt aagcacgcct
       61 aaaccatcga ctttgccata aagcagcgag tcaattactt caaccggttt gttgggtaac
      121 aaacgctgga tttcgggcaa catataagca atctgcggcc ctaataacac gacatcggca
      181 ttctgacctt tttcaccagc cagtgtttcc ggaaatgctt caataatgac cggaacttca
      241 tatttttctg cctgtgcgcg catttttgat accagtaaag aggtagacat gcccgcagaa
      301 caaaacagat aaatgtgttt cttttccata aaactgccct catcgacgat tatctgtcag
      361 ccagacactc cgcaagcctt aacctgcttc catgctctgg gtaacttgcg aaaccaacat
      421 gacttttttt gtggctgaaa ggagtatacg gtatagacct gagcaatagt atttctttga
      481 ccttgtaaat tgtctctcct tgacctgcgg ttatgaccct cttcacattt cgggcaaata
      541 attcgcgaag ataattaaga actcaggcca aaaaaaaccg gcgcaatggc cggtttccgt
      601 tgttactcaa gctttcagac gaagtgatta cttcgcagcc tgtggatcag tgtcgtattc
      661 agcacaggtc tgataaccgg agttgataac atgtccggta tcatctaacg cgacaaagta
      721 ggtttctgct ttaccatcac gttgacccag aatgtaggtc tggcaagtac cacgagcatg
      781 gatcatgctc acttcagacg aaggtttacc cgcaatttgt gcaacctgcg cccggctcat
      841 gccttttttg acgtctttca ccacaggctg tacaaactgg tctttggtac gatcataagc
      901 cgtacaaccc gccagcatgg ttaataccgc cgctgcactc agaattcctg ccatattctt
      961 gttcatattc cgtcctcttg tttatcagcg tgttagataa gcctggaata cattgggcgc
     1021 tttttcaagc ccgtgaacga aacggctccg ctttcagagg attcctgtat gacgttttaa
     1081 ccaccattca gcccgctgtc gcttgtcgtt tcagtagcaa cgggttagct ttaaggaagt
     1141 tttgtctttt ctgtctggag gggttcaatg acattgcaac aacaaataat aaaggcgctg
     1201 ggcgcaaaac cgcagattaa tgccgaagag gaaattcgtc gtagtgtcga ttttctgaaa
     1261 agctacctgc gaacttatcc gttcattaaa tcactggtgc tcgggatcag cggcggtcag
     1321 gactccacgc ttgccggtaa gctgtgccag atggcgatta atgagctgcg ccaggaaacc
     1381 ggcaacgaat cactgcaatt tattgccgta cgcctgccct atggtgttca ggccgacgaa
     1441 caagattgcc aggatgccat tgcctttatt caaccggatc gcgtattaac cgttaatatc
     1501 aagggcgcgg tattggctag cgagcaggca ttgcgggaag caggcattga actgagcgat
     1561 tttgtccgtg gcaatgaaaa agcgcgtgag cggatgaaag cacaatatag cattgcgggt
     1621 atgaccagcg gtgtcgtggt gggcaccgat catgcagcag aagccattac cggattcttc
     1681 actaaatatg gtgacggcgg tacggatatt aatccgctgt atcgtctcaa caaacgccag
     1741 ggtaaacagt tgctgacggc attaggttgc ccggaacacc tttataagaa agccccaacg
     1801 gccgatctgg aagatgatcg cccttctctg ccagatgaag tggcactcgg cgtgacctat
     1861 gacaatatcg acgactatct ggaagggaaa aacgtacctg aacaggtcgc cagaacaata
     1921 gagaactggt atctgaaaac cgaacataaa cgccgtccgc caattaccgt tttcgatgat
     1981 ttctggaaaa agtaataatt tgcacataat ggtttgtgcg aaaaagagta atttgttcac
     2041 gccggatgcg gcgtgaacgc cttattcgac ctacaaaact atgcaaattc aatatattgc
     2101 aggagtcgaa taggcctaac aggcgtagca cgtcagacgg tgtaaccttt gtcatcgacc
     2161 cgcctctttt ttaatcgctt cccgcctgtt acactggata gataaccagc attcggagtc
     2221 aacagtggta cggcgtttaa cttctccgcg gctcgaattt gaagctgcgg caatttatga
     2281 atatcccgaa catttacgtt cattccttaa tgacttaccc acccgaccag gggtgtatct
     2341 gtttcatggc gaaagtgaca ccatgccgct ctatatcggc aaaagcatta acatccgcag
     2401 ccgcgtcctt tctcatttac gtaccccgga tgaagccgcc atgctacggc aatcccgacg
     2461 catcagctgg atctgcaccg caggcgaaat cggcgctctg ctccttgaag cgcgattaat
     2521 caaagaacaa cagccgctgt ttaataaacg gttgcgccgc aatcgccagc tctgtgccct
     2581 gcaattaaat gaaaagcgcg tcgatgtggt gtatgccaaa gaggtggatt tttcacgagc
     2641 ccccaacctg tttggcctgt ttgccaatag gcgcgcagct ttgcaagcat tgcagagcat
     2701 cgctgatgaa caaaaacttt gttatggcct gttgggactg gaaccgttaa gtcgcggtcg
     2761 tgcatgtttt cgttcagcgc taaaacgttg cgccggagca tgctgcggta aagagagcca
     2821 tgaggaacat gcgctacgct tgcgccagtc tctggagcgt ttgcgggtgg tgtgttggcc
     2881 ttggcaaggt gcggtggcac tgaaagaaca gcacccggaa atgactcaat atcatattat
     2941 tcaaaactgg ctgtggctgg gggcggttaa ttcgctggaa gaagcgacaa cgttaattcg
     3001 gacacccgcc gggtttgatc acgacggtta taaaattctt tgtaagccgc tgctttccgg
     3061 taactatgaa attactgaac ttgatccggc gaatgaccag cgagccagtt gatttcactg
     3121 aacaacagtt taccttgcgg ttgtaaaagc cgcagggtgt gcctgccatc gaaccagcac
     3181 gcgccctggt cggtagtgag caatttatcg cccagttgcc acgccccgtt aatcacaaac
     3241 accaccccgc cgcgtgagcc aaaggtagta aacgtacgtt cggcaattct gactttcgcc
     3301 ttacagacat ccaggcgtgt catgatgttg aaatccatcg acatctgccc tgccgtcagt
     3361 ttcgctttta ccacctggtc cgctgcaaag gcaaaaggct gtaacggttt taaggtatgg
     3421 ttaaagcggt ctgcgctttc aaggagcatc tcgccgcctt ccagcaacgt cactatcctt
     3481 tccatgccgg gaaacaaaga aaactcccca ttcgccgcga tggatgcaat gctggcacgc
     3541 cagtaaaaat cacgttttgc cggtgggaaa gtgcaaattt cgcgcgtttc accggcagca
     3601 tttcgccaca gattcaccga cattttacgc atatcaaagt attccatact cgctcccggc
     3661 ccgttctttt cattgttatc gttcaggtaa ccgtggatgg ttatcggcaa ctcgggtgat
     3721 ttacgttagt ggtgatcagg ctttttttac aactgtttga gaagagaaaa gaaaaccgcc
     3781 gatcctgtcc accgcattac tgcaaggtag tggacaagac cggcggtctt aagttttttg
     3841 gctgaaagat tattcagcag ttgcaggcat tttacctttt gccgctggac gttctgtcag
     3901 acgcttctca aaattagcat taaattgctt tttctgttcc ggcgtcagga tgttgtaaat
     3961 tttgttctgg gtttccatgt gcgccagcat gttagctttg cgctgttctt ccatttttgc
     4021 gatctgcgct tcagcttttg ctttatcgaa ggtatcgctg gcaatgatgt catgcattgc
     4081 gcggcgttct tccagcggcg gacgtttcat ctggtcacgc tggcctttca tgatttcgcg
     4141 gatctgctgt ttctgcgcgt cggtcaggtt caggtctttg aacatcatgt cctgatgcgg
     4201 accgaacttg cctttgtggt gcatcatcgg cttcgcgtca gccggtgctg cggtagtggt
     4261 gtctgcggca tgggccaggt tagccgcgcc aagagccagg gtagaggcaa caaacagtgc
     4321 agttaattta cgcatattct atatccttcc tttcagttat ttattacggc tttcttaagt
     4381 agcgtgccgt gttgacgaga ttaactttac tgactttagc gtcaattaat cagagcaacg
     4441 gtaaaacaat gaaagtgtaa aaaacacttt tgcgccaatt atggagaaaa aaagaaaatt
     4501 tgatggagag tgatgagaga atattacaac acgatgattt tgcagagatt atgaagaact
     4561 ataccggatg actggtgata aataaagcaa ataaccagga ttaatctgta ttaatttata
     4621 agaaagcaac ttaatacccg cagaatgatt tctgcgggta agtattagct tattttttcg
     4681 agcattaatc ccgcgcgtaa tcccaacgct accaacggat tagggaataa cacatactct
     4741 acatcatggg ttacggtaaa acgttcctct ccgtcctgcg ccagcaatgt tcctttctca
     4801 aacggcataa aattcagcgt gtcacttgcc atatgcattt cgaaggacgg cgagtggcga
     4861 gtaatttgcg aaaccacccg ataacggagc ggcggtgttc tcacgatacc gacactctca
     4921 ccagatagca gcgcagcaat tgcgctggca gttactgcaa actggcgaag atcgttttgc
     4981 ccaaagggca acgctttgcc aagttccagc gtacaggcca gcgcgccaaa atgtctggcg
     5041 ctgaaatggg taaacgtacc accaggttcc tgatggaaca ccagcgcctc cagccccgcc
     5101 gcacccagcc acgtcagaaa tttctcgtcc cagggaatgt cgcgttgcgg taatacaccg
     5161 aactgcggat gcaaggagcc acgaattgcg gtatgtagat caaggtgcca gcgcacagat
     5221 tctttgccca ggtcataaaa atcttccagg cactgttcca gttcgcgcgc ccgacaggtt
     5281 tctccgcttt cagcaaatag ctgccaacga ccgccaaaca ttcgattcat atcgctatgg
     5341 caataacgtt tcccttgctt cagcgcagga ggattcccga ggatcaccag caaccgccaa
     5401 cgtaacggga tctcgccgtg agatatcgcg ccaagcaacg cgtccagcat ctccacaggt
     5461 gccgtctcat taccgtgtat tcccgctgaa atcaccagtg cgccttgcgg tggcgttaat
     5521 ggcgtcagtt ccagcacacc atcgcccagc cagcgccagc gaacgccgtt gatttctcgc
     5581 tcggtgataa ccggtttttt acccgttaag gtcagagcaa gaaaattatc cattgccccc
     5641 tccctcgcgc tggaacggat aaaccgaacc gagattcagt aattggctca atacatccag
     5701 tgcttcccgc ccttcgcgca gcaattgcgg gtcggccaga tcggcagcag taaggcgatc
     5761 gcggtagtaa cgatccaccc agtcattgag cgcattaaac agcgtatcgt tcatcatcac
     5821 cgccggattc accgcccggc gttcttcttc tgtcaatacc acccgcaacc gcaggcacgc
     5881 cgggccgccg ccattcgcca tgctttcacg taaatcaaag acttttagtt cgctaatcgg
     5941 gttgtcagcg gcaaggagtt cattgagata accccatact ccggcgtgtt cccgacactc
     6001 ctgaggcagc accaacatca tggaaccatc atcgcggctc aacagttggc tgttaaacag
     6061 ataggtagac accgcatccg acacggaaac ctgagttgcc ggaacttcta tcgccataaa
     6121 accgttcacc cgcgcacgca ggtttgccag taactgtgac tggcgagcga acgcctgttg
     6181 gtggcaaaac agcacctggc ggttactcac ggcaatcacg tcattatgaa aaacgccctg
     6241 gtcgataacg tccgggtttt gctgggcgaa aatcacctgt tggggattca cctgattcag
     6301 ccttgccacc gcctcgctgg cttcgcgagt ctgtcgcgcc ggataacggg aaggccgggt
     6361 atcattgcct tcttctcgcc cgtagacaaa aagttgcata cccggctcac cgtaatgacc
     6421 gccgagacga ttgtggtttg ccgccccctc atcaccgagc aacgctacct gcggcaacgc
     6481 cgaatgaacg ctaaatttct cttcgtcgtt aaaaatcgct tttaaaagtg attcggtgac
     6541 gtgcgcttcc agcgagcggt gaaatttatt gttcaggttg gcaacggtga gatgcacttt
     6601 gccatccagc gtatcggcag atggcgcgat cgttgccgca ttggctaccc acattggcga
     6661 agcggaactg acgctggaaa gccagtgcgg tgcctggcgt gcaacttttt ccagtacctg
     6721 ctcatcgcta ccactgaatc ccaactgacg cagcaccgga ataaacggac gctcgtgcgg
     6781 cgggatcacg gcctggggga atcccgcatc ggcaagggct ttcattttca gtaagccctg
     6841 ttttgccgcc tgtcgcgggt tagacacctg aaaacggtga cgggtagagg cttcattacc
     6901 aaacgacagg ccagcgtaat gatgcgtcag ccctaccagc ccgtcgaaat taacttccca
     6961 ggcgttcatc gcaccacctc atcggaaaaa tccagcccgg ggttaagcgt tgcgggcaaa
     7021 gttaacgagt ccgactccag gctcgccata ggccatgcgc agtaatctgc ggcataccag
     7081 gcgctggggc gatggttacc ggatgcacca atgccgccga atggcgcggt actggcagca
     7141 ccagtaagcg gtttgttcca gttaacaatc cccgcccgcg cctccagcaa cagttgatcg
     7201 aatttttccc gctcggggga aaccagaccg caagagagtc cgaagcgagt gttattcgcc
     7261 attagaatcg cttcctcgaa agaatcataa cgccagacgc gcaataacgg tccgaacacc
     7321 tcttcatctg gtacgccagc aacgcctgtc atttcaatga tccccggcgt cagcaacgat
     7381 gtctctgatt gtaataagcg cggcgcaagc agggttcgtc cgcccatcgc ttccagttgc
     7441 tgccaggcag taaccacctg ctgtgcggcc tgttcagaaa tcagcccgcc aataaacggc
     7501 tgcggttcgt catcccagtt gcccggcgtt aatcgctggc tgacggcaac cagacgagca
     7561 agaaacgcat cgccctgcgc tccgcttttg agcaataaac ggcgggcgca ggtgcagcgt
     7621 tgcccggctg tgacaaacgc cgactgaatg gtcagatgga cagccgcgtc gatatccgcc
     7681 acctcatcga taattagcgg attattaccg cccatctcaa gggcgagaat tttctccggc
     7741 tgaccggaga gctggcgatg cagctggtag ccggcattgg cgctaccggt aaacagcaaa
     7801 ccgtcgagat cctccagcgc actcagcgcc tgacccgttt cacgcccgcc ctgcaccagg
     7861 ttcagcacgc ccggcggcaa gccagcctgc tgccataaac gcattaccgc ttcgccactc
     7921 cacggtgtca gttcgctggg tttaaagata atggtgttac ctgccagcaa tgccggaacg
     7981 atatgtccgt tcggcaaatg accagggaaa ttatacggcc caaacaccgc cagcacgccg
     8041 tgcgggcgat gtcgcaggct cgccgcgccg tctggcattt cgctacgctg ctcgccggta
     8101 cgagcgtgat acgccttaat tgatatcgcg attttattga tcatcgccgt cacttcggtt
     8161 gccgcttccc agcgcggctt gccggtttct cgggcaatga ttgcggttaa ttcgccttta
     8221 ttcctttcca gcaatccggc aaagcgttcg acaacgacct gacgttcagc caatgagagc
     8281 cgcgcccagc gcggaaacgc cgcacgggct gcccgacaag cctgctcgac ctgagcggca
     8341 tcggcatcat tgccttgcca taacacctcg cccgataccg gattacgctt cacacgcgat
     8401 gcgccctggc ccgttatcca gtcaccgtta atccataaag tcatgctgtt ttctcctctg
     8461 cgcacaggcg caccagacga acgcgatccc ctgcgtggca tttgagggca tccagttgtg
     8521 cggcggttaa aatcaaacgc tcggttgccg gatcggtacg cgccagcacc acgcggaaat
     8581 ggtgataatt ttcattggcg accaggcagg ccggaaaatc gccctgcgca ggctgccctt
     8641 ctgccacttc caccagccga cttttacgaa tggcgcgcac gcggtcgatg tcacactcaa
     8701 gcgttggccc gccgtcgaag atgtcgatat agttgcggta acgaaaacct tctttctcca
     8761 gcaccgcgcg ggcaggcgcg gtttgcggat gtacctgacc gatgacgtcc tgggcttcct
     8821 gggataaaaa gtgggtatag atcggatgtt tcggcatcag ttctgcaata aatgcctttt
     8881 gcccggtgcc gcagagaaaa tcggcgcggc taaaatccat cgaaaagaag cgtttaccga
     8941 ggctttgcca gaacggtgaa tagccgtgtt cgtcaatcac cccgcgcatt tcagcaacca
     9001 ctttgtcatt aaacttgtcg cgaaaagccg ccataaacat aaagcgcgat ttcgacagca
     9061 aatagccgtt gccctctttg cgccagtccg ggtcgagaaa cagcgtgcac agctcgctgc
     9121 tgccggtgtg atcgttactg agaaacagcg tcggcaatgc gttatagaca ttcagctctt
     9181 ttgaggcgtg aaccaacgtg ccgacgcgat agttgtacca gggatcgttc agcccaaccg
     9241 ccacctcaat ggcacaaatc cccgccacgg tgcctgtctc gctatcttcc agcacgaaca
     9301 catagccctg ctcacttttg ggcagttcgc cttgccaggt tttgattgcc ctttcgatac
     9361 gcgccgaaag cgtggcttca ttggcgggaa gcgacgtcag gccgccgccc gttttgctgg
     9421 caagctgcat cagcgccgag acatctgatc gctcaacggg acggatgacc atcatgatga
     9481 acctcggcta acaaagtgtt cgcaagcagc tgcaaagcga tccagtccgg tcgtcacctc
     9541 ttcttcgctg acattgagcg caggcgcaaa acgcaccacg ttgccacccg caatcagtac
     9601 catcacgcct gctttcgccg cttcctgaga gatctgtttc gcttgcccgg cgtaatcggc
     9661 attcagtaca cagccaatca gcaaacctaa gccgcgaact tcactgaaca aaccatagcg
     9721 gtgattaata gtattaagac gctcaacaaa ccagtcgtga cgctgtttaa cgccattaag
     9781 catctctggt gtgttgatga gctccagcac tttgcctgcc accgccgagg ccagcggatt
     9841 accgccatag gtggtgccat gagtgccaac ggtcatcacg cgggcgcact cttcggttgc
     9901 caacaacgca ccgaccggga aaccgccgcc cagcgctttg gcggtagtta acagatcagg
     9961 cgtcacgccg tagtgcatat aggcatacag ttccccggtg cgcccgacgc cggtttgtac
    10021 ttcatcaaaa atcaacagcg cattgtggcg gttacacaat tcacgcagac cttgtaaaaa
    10081 cgcgttgctg gctggcacca caccgccttc cccctggatg ggttcgacaa tcaccgcaca
    10141 ggtagagtcg tcaatcagcg cgctggcaga gttaatatcg ttatatgcag catgacgaat
    10201 atccgccggc agtggcgcaa aatcctgtga ataggctggc tgcccacccg cactgacagt
    10261 aaacagcgtg cgaccatgaa acgcattttt gaacgccacg atgccgctct tatggctgcc
    10321 gtagcggtcg tgagcgaatt tacgcgccag ttttagcgcc gcttcgttgg cttccgcacc
    10381 ggagttacaa aagaagacgc gatcggcaaa cgtggcgtcg atcaattttt tcgccagtcg
    10441 cagtaccggc tcgttggtgt aaccgttgct ggtatgccag aacttactcg cctgttcgtt
    10501 cagcgcttca cgcagttccg gatgcgcatg gcccagcgcg ttcaccgcaa tgccacccgc
    10561 gaagtcgata tactctttcc cctgctgatc ccacaagcgc gaaccttcgc cacgtaccgg
    10621 tataaagggt gccggagcgt aaacaggtat catccattca tcaaagtttt cacgcgtaat
    10681 tggctgagac atagcgacct ctacagtaaa taattcgtta tttatatgtt aacaataagt
    10741 aatgtttgcg ctgtaaatgt agattgcagg gttcgtgcca gccagtgata aaagtgcata
    10801 aacggcggag gctaactgga aatcaaggag ttataaccaa accatatgca tttaaagtgc
    10861 atataaagtg aatacgtttg cgatgtgggt gaataaaaag aataaaaaac gcaatgttat
    10921 gcagaagtaa aatataattc tggaattgtg atcattgacg aaatttactg gaaattactg
    10981 cgccattctg acgcagcgcg caccaaaagc gggcattttt tgcgccatcg ttgacatcat
    11041 taacaaccat cgatcaaatc acttaacaac aggcggtaag caacgcgaaa ttctgctacc
    11101 atccacgcac tctttatctg aataaatggc agcgactatg aaatttgtct cttttaatat
    11161 caacggcctg cgcgccagac ctcaccagct tgaagccatc gtcgaaaagc accaaccgga
    11221 tgtgattggc ctgcaggaga caaaagttca tgacgatatg tttccgctcg aagaggtggc
    11281 gaagctcggc tacaacgtgt tttatcacgg gcagaaaggc cattatggcg tggcgctgct
    11341 gaccaaagag acgccgattg ccgtgcgtcg cggctttccc ggtgacgacg aagaggcgca
    11401 gcggcggatt attatggcgg aaatcccctc actgctgggt aatgtcaccg tgatcaacgg
    11461 ttacttcccg cagggtgaaa gccgcgacca tccgataaaa ttcccggcaa aagcgcagtt
    11521 ttatcagaat ctgcaaaact acctggaaac cgaactcaaa cgtgataatc cggtactgat
    11581 tatgggcgat atgaatatca gccctacaga tctggatatc ggcattggcg aagaaaaccg
    11641 taagcgctgg ctgcgtaccg gtaaatgctc tttcctgccg gaagagcgcg aatggatgga
    11701 caggctgatg agctgggggt tggtcgatac tttccgccat gctaatccgc aaacagcaga
    11761 tcgtttctca tggtttgatt accgctcaaa aggttttgac gataaccgtg gtctgcgcat
    11821 cgacctgctg ctcgccagcc aaccgctggc agaatgttgc gtagaaaccg gcatcgacta
    11881 tgaaattcgc agcatggaaa aaccgtccga tcacgccccc gtctgggcga ccttccgccg
    11941 ctaatttagc agctctcctg gctcaaactg ggtcaggaga attaaccttg agaaaaatca
    12001 acaaactgtc agtaatgatt tgttgcctgc cgtcctttgt tataccgtca ctgcgttttt
    12061 agttgtctga ccacttctct attatcaagt ttgatatagg aaactccacg atgaacgctg
    12121 agcgtaaatt tcttttggcc tgtcttattt ttgcgctggt catttacgct atccacactt
    12181 tcggtttatt cgatctgctc accgatttac cccacttaca gacactcatc cgccagagcg
    12241 gattgttcgg ttatagcctc tatattctgt tattcattat tgccactctc tttctgttac
    12301 caggaagcat actggtgatc gctggaggaa tagtttttgg cccgctctta gggacactac
    12361 tctcattaat tgccgccacg ctcgcctcct cgtgctcatt cctgctggcg cgctggctgg
    12421 gacgtgattt actgctgaaa tacgttggtc atagccatac ctttcaggcc attgaaaaag
    12481 gcattgcgcg taacggtatt gattttctta ttctgacccg cttaatcccg ttgtttcctt
    12541 acaatattca aaattacgct tacggattaa ccacaatcgc cttctggcct tataccctta
    12601 tttcggcact cacgaccctg cccggtattg ttatttatac cgtgatggca agcgatctcg
    12661 ccaatgaagg cattacgctg cgctttattt tacaactctg tctggcgggc ctggcgctgt
    12721 ttattctcgt ccagctcgca aaactctacg cccgacacaa acatgtggat ctgtctgctt
    12781 cgcgccgcag cccacttact cacccaaaaa atgaaggata gaacgatgtc gcaacattat
    12841 tcagtgtcat ggaaaaaagg actggctgca ctctgcttac tggctgttgc cgggcttagc
    12901 ggctgcgatc aaaaagagaa tgcggcggca aaagtggaat acgacggact ttcgaacagc
    12961 caaccgctgc gcgtcgatgc caataaccat acggtaacca tgctggtgca aattaatggt
    13021 cgtttcctca ccgacgacac tcgtcacggc attgtgttta aagatggctc caacggacat
    13081 aaatcgctgt ttatggctta tgcgaccccg aaagcatttt atgaagccct gaaagaggca
    13141 ggtggtacgc cgggcgaaaa catgacgatg gataataaag aaacgactca tgtcacaggc
    13201 agcaaactgg atatttcggt caactggcaa ggggcggcaa aagcgtattc cttcgatgaa
    13261 gtgattgttg acagtaatgg caagaaactg gacatgcgct ttggcggtaa tttaacggca
    13321 gcagaagaga agaaaacggg ttgcctggtg tgtctggata gctgcccggt cggcatcgtc
    13381 agcaatgcaa catacactta tggtgcggtg gaaaaacgcg gcgaagtgaa attcaaaggc
    13441 aatgcctcag ttctcccggc ggataacacg ctggcaacgg ttacctttaa aatcaccgaa
    13501 taaagccagg ataaaggatg atgaagatac aatcgcgtaa aatctggtac taccgtataa
    13561 ccctcatcat cctgttgttc gctatgctgc tcgcatgggc gctgcttccc ggcgtccatg
    13621 agtttatcaa tcgcagcgtt gcggcgtttg ccgccgtgga ccaacagggt atagaacgct
    13681 ttattcagtc ttacggcgca ctggcggcag ttgtctcgtt cttgttgatg attttacagg
    13741 ccattgctgc accgctacct gcgtttttga tcacctttgc caatgcgtcg ctgtttggcg
    13801 cgttctgggg cggcttgctg tcgtggacca gttcgatggc cggcgcggcg ctgtgctttt
    13861 ttatcgccag agtgatgggc cgcgaagtgg tggaaaaatt aaccggcaaa accgtgcttg
    13921 acagtatgga cggctttttc actcgctacg gcaaacacac catcctggtc tgtcggttat
    13981 tgccttttgt ccctttcgat ccaatcagct atgctgccgg tctgacttca atacgttttc
    14041 gctcgttttt tatcgccacc gggcttggtc aattaccggc gactattgtt tattcctggg
    14101 cgggcagcat gttaacaggc ggtactttct ggtttgtcac cggactgttt attctgtttg
    14161 ccctgaccgt ggtgattttt atggcgaaga aaatatggct tgagcgccag aagaggaatg
    14221 cctgatgggt ttaccgccgc ttagcaaaat tcctttcatt ttacgtccgc aggcgtggct
    14281 gcatcgtcgc cattacggcg aggtgctaag cccgattcgc tggtgggggc ggatcccgtt
    14341 tatcttttat ttggtgtcaa tgtttgttgg gtggctggag cgcaaacgct caccgctcga
    14401 tccggtagtg cgatcgcttg tcagcgcgcg cattgcgcaa atgtgcctgt gtgagttttg
    14461 cgtggatatc accagtatga aagtcgccga gcgtaccggc agcaccgata aactgctggc
    14521 agtggctgac tggcggcaaa gcccgctctt tagcgatgaa gaacggctgg cgctggagta
    14581 cgccgaagcc gcaagcgtca caccgccaac agtcgatgat gccctgcgta cccgactggc
    14641 tgcgcatttt gacgctcagg cgctcaccga actgacggca ttgatcggcc tgcaaaacct
    14701 gtcggctcgt tttaattctg ccatggacat tcccgctcag gggctgtgcc gtattcctga
    14761 aaaacgttct taaggagaga tgatgcgcca ttgtgggtgg ttgctgggat tgttatcgct
    14821 gttttctttg gcaacacatg ccagtgactg gcaggaaatt aaaaatgagg ccaaagggca
    14881 aaccgtctgg tttaacgcct ggggcggcga taccgcaatt aaccgctatc tcgactgggt
    14941 tagcggcgag atgaaaaccc attacgctat aaacctgaag attgttcgtc tcgcggatgc
    15001 cgcagacgcg gtgaagcgca ttcagaccga agctgctgcc ggtcgtaaaa cgggcggctc
    15061 ggtggatctg ctctgggtga acggcgaaaa cttccgcacc ttaaaagagg ccaagttatt
    15121 acaaacgggc tgggcggaga ctctgcccaa ctggcgctat gtcgacacac agctgccggt
    15181 gcgggaagat ttttcagtgc ctacagaagg ggctgaatcg ccctggggcg gcgcacaact
    15241 gacgtttatc gcccgccgcg atgttacgcc acagccacca caaacgccgc aagccttact
    15301 ggagtttgct aaagccaatc ccggcacggt tacctatccg cgcccaccgg actttaccgg
    15361 cacggcgttt cttgaacagt tgctgattat gctgacgccc gatcccgccg cgttaaaagt
    15421 tgccccggat aaagccactt tcgcccgcgt cactgcgccc ttgtggcaat atcttgacgc
    15481 gctacatccc tatttatggc gcaaaggaaa ggatttcccg ccttcgcccg cgcggatgga
    15541 tgctctgctg aaagccggca cattgcgcct gtcgctgacc tttaaccctg ctcatgccca
    15601 gcaaaaaatc gccagcggcg atttgcctgc aagcagttac agttttggtt ttaaccaggg
    15661 gatgattggc aacgtgcatt tcgtcaccat tcctgctaac gcaaatgcca gtgctgcggc
    15721 gaaggtggtt gccaacttcc tgctctcacc caatgcgcaa ctgcgtaaag cggatcccgc
    15781 tgtctggggc gatccttctg ttctcgatcc gcaaaaactg cctgatgggc agcgcgaaac
    15841 attgcaatca agaatgccgc aagatctgcc gccggtactg gctgaaccgc acgcaggttg
    15901 ggtaaatgcg ctggaacaag aatggctaca ccgttacggt acgcattaat ctttttgctg
    15961 tgggcgatgg tggcggtgat ttatgcacca ctgatcccgg cagctctcac gctgatatcg
    16021 cctgctttgt cgttgacaca ctggcaggcg ttatttgccg atccacagtt accgcaggca
    16081 ttactggcaa cgctggtgtc gacaaccatc gcggcggtcg gggcgttgtt gattgccctg
    16141 ctggtgattg tggcgctgtg gccttggccg aaatggcagc gtatgtgtgc ccgtctgccg
    16201 tggctgctcg ccattcccca tgtggctttt gccaccagcg cccttctgct ctttgctgac
    16261 ggagggctgc tttatgacta tttcccgtat ttcactccgc taatggacaa attgggtatt
    16321 gggctgggcc tcacccttgc agtgaaagaa agcgcatttc tgctgtggat cttagcggca
    16381 gtattgagcg aaaaacggct gttgcagcag gtcattgtgc tggattcgct gggctacagc
    16441 cgctggcaat gcctgaactg gctgctcttg ccctccgtcg cacctgcact ggcaatggcg
    16501 atgttggcga ttgttgcctg gtcgctttcg gtcgtggatg tggcgattat tcttgggcca
    16561 ggtaatccac cgacgctggc agtaattagc tggcagtggt taactcaggg cgacgcggat
    16621 caacaaacga aaggcgcact cgccagtctg ctgctgatgc tgttactcgc cgcctacgtt
    16681 ttgctgagct atctgctatg gcgcagctgg cggcgcacta ttccccgcgt agatggcatt
    16741 cgcaagcctg ccacaccttt attaccaggc actacgctgg cgagtttttt acccttaacc
    16801 ggtgtgctgt gtgtggttct gctggcgatc ctcgcggatc agtcgacgat caatagtgaa
    16861 gcactcatca acagcctgac aatggggctg gtggcgacat tcatcgcttt gctcctgtta
    16921 ctgctgtggc tggaatgggg gccacagcgt cgccagttgt ggctctggtt gcccattcta
    16981 ttgcccgctc tgccactggt ggcgggccag tacacgctgg cgctatggct gaacctggat
    17041 ggaagctgga cggcggtggt ctgggggcat ctgctgtggg taatgccgtg gatgctgttt
    17101 atcctgcaac ccgcctggca gcgcattgat tcacggttaa ttttgattgc gcaaacactg
    17161 agctggtcgc gggccaaaat cttcttttac gtgaaatgtc cgcttatgct acgccctacg
    17221 ctgattgcct tcgcggtggg attttcagtc agtattgcgc agtatatgcc gacgctgtgg
    17281 ctgggcgcgg ggcgttttcc gacgctcacc actgaggcgg tggcattaag cagcggcggc
    17341 agcaatggta ttttcgccgc ccaggcttta tggcaactgc tattaccgct tattattttt
    17401 gccctgaccg cgttagtcgc aaaatgggta ggttatgtca gacaaggact ccgctaatgc
    17461 tctgcgtgaa aaatgtttcg ctacgtttac cagaaagccg cttgctgaca aacgttaact
    17521 ttacggtgga taaaggtgac attgtcacgt taatggggcc gtctggctgt ggaaaatcca
    17581 ctctgttttc atggatgatt ggtgcactgg ctggacagtt ttcttgtaca ggtgagctat
    17641 ggctcaatga gcaacggatt gacatgctac ccaccgcaca gcgtcagatt ggcattcttt
    17701 tccaggatgc actgttattt gaccagttca gcgtcgggca aaatttactg ctggcgctac
    17761 cgtcgacgct caaggggact gccaggcgca atgccgtgaa agacgcgctt gatcgagcgg
    17821 gactggctga aacttatcac caggaccctg cgacattatc aggcggtcag cgagcgcggg
    17881 tggccctgct gcgcgccctt ctcgctcagc caaaagcgtt actcctggat gagccattca
    17941 gccgtcttga tgtggctctg cgcgataatt ttcgccagtg ggtgttcagc gaagttcgcg
    18001 aactggcgat ccccgtcgtt caggtaacgc acgatctcca ggatgttcct gctgatagtt
    18061 ctgttctgga tatggcgcag tggtcagaaa attacaacaa actgcgataa cgcaaagttt
    18121 ttctcaatgc gtcagttcag aatggcgcac tcaaaactac aatgtcggga ttttcgatga
    18181 aacgtgtttc tcaaatgacc gcgctggcaa tggctttagg gctggcttgc gcttcttcgt
    18241 gggccgctga actggcgaag cctcttacac ttgaccagct tcaacaacaa aatggcaaag
    18301 cgatagatac tcgccccagc gcgttttata acggctggcc acaaacctta aatggccctt
    18361 ctggtcatga acctgccgcc ttaaacctct ctgccagctg gctcgacaaa atgagcaccg
    18421 aacagctcaa cgcgtggatc aagcaacata acctgaaagc cgatgccccg gtggcgctgt
    18481 acggtaatga caaagatgtc gacgccgtca aaacgcgact gcaaaaagca ggttttacgc
    18541 atatctccat tctgagtgac gcgctaagcg aaccttcccg tctacaaaaa ctgccgcact
    18601 ttgagcagct ggtttatccg caatggctgc acgacctgca acaaggtaaa gaggttacgg
    18661 cgaaacctgc cggtgactgg aaagtcattg aagcggcctg gggcgctcct aagctttacc
    18721 ttatcagcca tattcccggt gctgactaca tcgataccaa cgaagtggaa agcgaaccgc
    18781 tgtggaacaa agtttctgat gaacaactaa aagcgatgct ggcaaaacac ggcattcgcc
    18841 atgacaccac ggtcattctg tacgggcgtg acgtatacgc tggagcgcgt gtggcgcaaa
    18901 ttatgctcta cgcaggcgtg aaagatgtgc gtctgctgga tggcggctgg caaacctggt
    18961 ccgacgcggg actgcctgtt gagcgaggaa cgccaccgaa agtgaaagcg gaaccggatt
    19021 tcggcgtgaa gatcccggca caaccgcagt tgatgctcga tatggaacag gcccgtggac
    19081 tgctgcatcg ccaggatgca tcgctggtaa gcattcgttc gtggccagaa tttatcggta
    19141 cgaccagcgg ttacagctat attaaaccaa aaggtgaaat agccggagca cgttggggac
    19201 acgctggtag cgactcgacg catatggaag attttcataa cccggatggc accatgcgca
    19261 gcgccgatga tattaccgct atgtggaaag cgtggaatat caaaccagag cagcaagttt
    19321 cattctactg cggcaccggc tggcgcgcgt ccgaaacctt tatgtacgca cgcgccatgg
    19381 gttggaagaa tgtttccgtg tatgacggcg gctggtacga atggagcagc gatccaaaaa
    19441 atccggtagc aaccggtgaa cgcggcccgg acagtagcaa ataacattgc aattactgac
    19501 gctggtgcga cttcagcgtc agatacccac tccacacccg cgtaaaggtc gtcatccagc
    19561 acaacgcacc gaagatccac gcaaaccacg caaaccatgc agggaataaa catcccagca
    19621 caaacagtaa gatcgtttct gtgccttcgg ttaacccgcc cagataataa aacgatttat
    19681 gcgcataacc gggattatca atctgatgtt tcgccgccag cgcagcaaaa gcgagaaaac
    19741 tgctgcctgt gccgataaac gcaaacagca gccagccgcc tgccagggcg ttttgctgcg
    19801 gtgcggcgag gataaaccca aaaggtacca gcgcgtaaaa aagaaaatcg agagaaatat
    19861 caagaaagcc gcccgcgtcg gtaagccctc tgcgccgcgc cagcgcaccg tccagaccat
    19921 caagcagcct gttcaacaaa atgacgacaa gcgccgccag ataccagccc agcgccagaa
    19981 aaggcagtgc cagcacgccg atggcaaatc ccactaatgt taaaccatct ggcgtaatgc
    20041 ccggtttatc aagaacccgc acgcactgat gcaacaacgg tttaatccgg ggatgaagat
    20101 ggcggtctag cacaggcact ccttaaatat aaagcctttc tgattgagca acagtgcgga
    20161 tattatggca tttttcgctt atctgcccgt gtgtaattta tgaaaatgat tgaagttgtt
    20221 gccgccatca ttgaacgtga tggcaaaatt ttactcgcgc aacgccccgc ccagagcgat
    20281 caggcgggat tatgggagtt tgccggtggt aaagtcgagc cggatgaaag ccagcggcag
    20341 gcgctggtgc gtgagttacg cgaagaactg ggcatcgaag caactgtggg tgagtatgtt
    20401 accagccatc agcgagaagt ttcgggccgg attatccatc ttcatgcctg gcacgtaccc
    20461 gacttccacg ggacgttaca ggcacatgaa catcaggcgc tggtctggtg ctcacctgaa
    20521 gaggcgctgc aatatccgct ggcccctgct gacattccat tattagagtc gtttatggct
    20581 ttacgcgccg ccagaccagc ggattagtgc taagggtttt gtcatcccgc tggcattgca
    20641 gcagtattcc ctcggcttta attaccgcgc cttcagaata atttttatcc tgataaacgc
    20701 agcactgagt acagggctgc gctgactgcc cgcctgaact gaacacttct ggcggtacgt
    20761 ttacctccac gtccggacga taatgcgggt tagccagtgc aattaatgga aatgctaata
    20821 ctacggcgaa caatgctcga ctcacaggga actccttaac gttattgtct ctgctactga
    20881 taacggtagc cgggtggcaa aactttagcg tctgagttat cgcatttggt tatgagatta
    20941 ctctcgttat taatttgctt tcctgggtca tttttttctt gcttaccgtc acattcttga
    21001 tggtatagtc gaaaactgca aaagcacatc acataaacaa cataagcaca atcgtattaa
    21061 tatataaggg ttttatatct atggatcaga catattctct ggagtcattc ctcaaccatg
    21121 tccaaaagcg cgacccgaat caaaccgagt tcgcgcaagc cgttcgtgaa gtaatgacca
    21181 cactctggcc ttttcttgaa caaaatccaa aatatcgcca gatgtcatta ctggagcgtc
    21241 tggttgaacc ggagcgcgtg atccagtttc gcgtggtatg ggttgatgat cgcaatcagg
    21301 tacaggtcaa ccgtgcatgg cgtgtgcagt tcagctctgc catcggcccg tacaaaggcg
    21361 gtatgcgctt ccatccgtca gttaacctct ccattcttaa attcctcggc ttcgaacaaa
    21421 ccttcaaaaa tgccctgact accctgccga tgggcggtgg taaaggcggc agcgatttcg
    21481 atccgaaagg aaaaagcgaa ggtgaagtga tgcgtttttg ccaggcgctg atgactgaac
    21541 tgtatcgcca cctgggcgcg gataccgacg ttccggcagg tgatatcggg gttggtggtc
    21601 gtgaagtcgg ctttatggcg gggatgatga aaaagctctc caacaatacc gcctgcgtct
    21661 tcaccggtaa gggcctttca tttggcggca gtcttattcg cccggaagct accggctacg
    21721 gtctggttta tttcacagaa gcaatgctaa aacgccacgg tatgggtttt gaagggatgc
    21781 gcgtttccgt ttctggctcc ggcaacgtcg cccagtacgc tatcgaaaaa gcgatggaat
    21841 ttggtgctcg tgtgatcact gcgtcagact ccagcggcac tgtagttgat gaaagcggat
    21901 tcacgaaaga gaaactggca cgtcttatcg aaatcaaagc cagccgcgat ggtcgagtgg
    21961 cagattatgc caaagaattt ggtctggtct atctcgaacg ccaacagccg tggtctgtac
    22021 cggttgatat cgccctgcct tgcgccaccc agaacgaact ggatgttgac gccgcgcatc
    22081 agcttatcgc caatggcgtt aaagccgtcg ccgaaggggc aaatatgccg accaccatcg
    22141 aagcgactga actgttccag caggcaggcg tactgtttgc accgggtaaa gcggctaatg
    22201 ctggcggtgt agcaacgtca ggccttgaaa tggcgcaaaa cgctgcgcgc ctgggctgga
    22261 aagccgagaa agttgacgca cgtttgcatc acatcatgct ggatatccac catgcctgtg
    22321 ttgagcatgg tggtgaaggt gagcaaacca actacgtgca gggcgcgaac attgccggtt
    22381 ttgtgaaggt tgccgatgcg atgctggcgc agggtgtgat ttaagttgta aatgcctgat
    22441 ggcgctacgc ttatcaggcc tacaaatggg cacaattcat tgcagttacg ctctaatgta
    22501 ggccgggcaa gcgcagcgcc cccggcaaaa tttcaggcgt ttatgagtat ttaacggatg
    22561 atgctcccca gggaacattt cttatgggcc aacggcattt cttactgtag tgctcccaaa
    22621 actgcttgtc gtaacgataa cacgcttcaa gttcagcatc cgttaacttt ctgcgatagc
    22681 agcagatatg ccagtaaaga aatcccatta gactattttt ttgataatct tcttcgcttt
    22741 cgaacaactc gtgcgccttt cgagaagcaa gcattatata atgccaggcc agttcttctt
    22801 caattgtccc gttttgaaaa gctgtgcttg atatcgaggt catccatgat aattccgccg
    22861 cccatattag cttcgccgag gatttaccgg agcgattatt agcgcaatca gagatatagt
    22921 ctgagggaaa aacagcaaat ttattcaaca aggcgataac ctgctctggg gcttcctcca
    22981 tgtttgcttt aaaggtattg gctccatggt cgccagaaag aaaatgctcc attaaggcac
    23041 aataactttc gctatcttcg ataccccatt gatcctctaa agactcgcgt cttttactta
    23101 tgatatcgat cgagtcaaag ggaagcacat gatattggaa ggtatctttg ccaggttcag
    23161 gctttcgcgg ccagaactcc agcgtttcag accattgctt atgatagaac cgataaggtg
    23221 cgatcaattg tagcgcctgt aacttctcga tactgagcgg ctcaatacct ttagcctgat
    23281 aataatgcag ttgttctttt tttgctttaa aaccggcccg aacaataagc cccatcataa
    23341 ttaatagata aagaaaagag catcccgcag taatcaggcc tctttcattc aaaccgttgg
    23401 atgttatcgc tgcgaataca aacattacag cgacaacaca tgttaaatac cccccccact
    23461 tacaaagcag catg
//
LOCUS       DBANUU010000075        23346 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000075 DBANUU010000000
VERSION     DBANUU010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.075"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(131..475)
                     /gene="yoaB"
                     /locus_tag="ACIE68_002828"
     CDS             complement(131..475)
                     /gene="yoaB"
                     /locus_tag="ACIE68_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFX5909116.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            607..2517
                     /gene="yoaA"
                     /locus_tag="ACIE68_002829"
     CDS             607..2517
                     /gene="yoaA"
                     /locus_tag="ACIE68_002829"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFX5909117.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2575..3270
                     /gene="tsaB"
                     /locus_tag="ACIE68_002830"
     CDS             2575..3270
                     /gene="tsaB"
                     /locus_tag="ACIE68_002830"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFX5909118.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3310..3891
                     /gene="yeaY"
                     /locus_tag="ACIE68_002831"
     CDS             3310..3891
                     /gene="yeaY"
                     /locus_tag="ACIE68_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFX5909119.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADMN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4096..5781
                     /gene="fadD"
                     /locus_tag="ACIE68_002832"
     CDS             4096..5781
                     /gene="fadD"
                     /locus_tag="ACIE68_002832"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFX5909120.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            5863..6978
                     /gene="rnd"
                     /locus_tag="ACIE68_002833"
     CDS             5863..6978
                     /gene="rnd"
                     /locus_tag="ACIE68_002833"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFX5909121.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKVFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7032..7997)
                     /gene="yeaX"
                     /locus_tag="ACIE68_002834"
     CDS             complement(7032..7997)
                     /gene="yeaX"
                     /locus_tag="ACIE68_002834"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFX5909122.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNHYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8053..9177)
                     /gene="yeaW"
                     /locus_tag="ACIE68_002835"
     CDS             complement(8053..9177)
                     /gene="yeaW"
                     /locus_tag="ACIE68_002835"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFX5909123.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVQDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            complement(9209..10819)
                     /gene="yeaV"
                     /locus_tag="ACIE68_002836"
     CDS             complement(9209..10819)
                     /gene="yeaV"
                     /locus_tag="ACIE68_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFX5909124.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            complement(11070..12155)
                     /gene="dmlA"
                     /locus_tag="ACIE68_002837"
     CDS             complement(11070..12155)
                     /gene="dmlA"
                     /locus_tag="ACIE68_002837"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFX5909125.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            12258..13181
                     /gene="dmlR"
                     /locus_tag="ACIE68_002838"
     CDS             12258..13181
                     /gene="dmlR"
                     /locus_tag="ACIE68_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFX5909126.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            13308..13946
                     /gene="leuE"
                     /locus_tag="ACIE68_002839"
     CDS             13308..13946
                     /gene="leuE"
                     /locus_tag="ACIE68_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFX5909127.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            14119..14478
                     /gene="yeaR"
                     /locus_tag="ACIE68_002840"
     CDS             14119..14478
                     /gene="yeaR"
                     /locus_tag="ACIE68_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFX5909128.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYFGYPDEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            14482..14664
                     /gene="yoaG"
                     /locus_tag="ACIE68_002841"
     CDS             14482..14664
                     /gene="yoaG"
                     /locus_tag="ACIE68_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFX5909129.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            14812..15060
                     /gene="yeaQ"
                     /locus_tag="ACIE68_002842"
     CDS             14812..15060
                     /gene="yeaQ"
                     /locus_tag="ACIE68_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFX5909130.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(15119..15193)
                     /gene="yoaJ"
                     /locus_tag="ACIE68_002843"
     CDS             complement(15119..15193)
                     /gene="yoaJ"
                     /locus_tag="ACIE68_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFX5909131.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(15196..15294)
                     /gene="yoaK"
                     /locus_tag="ACIE68_002844"
     CDS             complement(15196..15294)
                     /gene="yoaK"
                     /locus_tag="ACIE68_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFX5909132.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            complement(15327..16352)
                     /gene="dgcP"
                     /locus_tag="ACIE68_002845"
     CDS             complement(15327..16352)
                     /gene="dgcP"
                     /locus_tag="ACIE68_002845"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFX5909133.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            16535..16789
                     /gene="yoaF"
                     /locus_tag="ACIE68_002846"
     CDS             16535..16789
                     /gene="yoaF"
                     /locus_tag="ACIE68_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFX5909134.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            complement(16811..17158)
                     /gene="yeaO"
                     /locus_tag="ACIE68_002847"
     CDS             complement(16811..17158)
                     /gene="yeaO"
                     /locus_tag="ACIE68_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFX5909135.1"
                     /translation="MNIQCKRVYDPAEQSDGYRILVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(17213..18394)
                     /gene="nimT"
                     /locus_tag="ACIE68_002848"
     CDS             complement(17213..18394)
                     /gene="nimT"
                     /locus_tag="ACIE68_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFX5909136.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     FNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            18491..19312
                     /gene="nimR"
                     /locus_tag="ACIE68_002849"
     CDS             18491..19312
                     /gene="nimR"
                     /locus_tag="ACIE68_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFX5909137.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTHRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            complement(19269..19715)
                     /gene="yeaL"
                     /locus_tag="ACIE68_002850"
     CDS             complement(19269..19715)
                     /gene="yeaL"
                     /locus_tag="ACIE68_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFX5909138.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            19885..19989
                     /gene="yoaI"
                     /locus_tag="ACIE68_002851"
     CDS             19885..19989
                     /gene="yoaI"
                     /locus_tag="ACIE68_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFX5909139.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            complement(19990..20493)
                     /gene="yeaK"
                     /locus_tag="ACIE68_002852"
     CDS             complement(19990..20493)
                     /gene="yeaK"
                     /locus_tag="ACIE68_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFX5909140.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(20536..22026)
                     /gene="dgcJ"
                     /locus_tag="ACIE68_002853"
     CDS             complement(20536..22026)
                     /gene="dgcJ"
                     /locus_tag="ACIE68_002853"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFX5909141.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            complement(22207..>23346)
                     /locus_tag="ACIE68_002854"
     CDS             complement(22207..>23346)
                     /locus_tag="ACIE68_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310521.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFX5909142.1"
                     /translation="FTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKS
                     NDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNL
                     SSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIAL
                     LCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVH
                     DVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIV
                     DIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPE
                     QMTISIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT         6013 a         5647 c         5955 g         5731 t
ORIGIN      
        1 gaaattataa aaataaataa tgaattagca ggagcatcaa caggtaatat cttaatctct
       61 ggtcgattta tgtaaaaccg accagtatta tcgataaatt atcgtctgac ttattttgct
      121 tcgataaagc ttacaccgca gccacaatct taatttcaac tttatacttc gggttcatca
      181 aacccgcttg taccgtgcag cgcaccggcg catgacccgc gacaacccag gcatcccacg
      241 ctttattcat cgccgcgaag tcgtttttat cggccaggaa aatggtggca tccagaatgc
      301 tcgatttatt gctgccctgt ttttccagca cggcatcaat ctgcgccagc gtgttggcgg
      361 tttgctcaaa ggcatcggcg tcgaggtttt ccggtacacc agtgtagtag agcgtgttgt
      421 tgtggattac cacatcagac cagcgggctt cagcatcgat acgaacgata gtcatcattt
      481 tgtcctcatt atccttccat tttcgtgctg caaagactgc cacaatgtga ggtcgccgtc
      541 accacttcaa ctggcgaaag cgccctcatc ctgacataat ccctgttcaa atcaacaggg
      601 ggtagtgtga cggacgattt tgcaccagac ggtcagctgg cgaaagcgat accaggcttt
      661 aagccgcgag aaccacagcg acagatggcg gtagccgtca cccaggcgat agaaaaaggc
      721 cagccgctgg tggtggaagc aggaaccggt acgggcaaaa cctacgctta cctggctcct
      781 gcgctgcggg cgaaaaagaa agtcattatc tcgaccggct caaaagcgtt gcaggatcag
      841 ctctacagcc gcgatttgcc aacagtctca aaggcattga aatatacggg caacgtggcg
      901 ctgctgaaag ggcgctcaaa ctacctctgc ctcgagcgtc tcgaacagca ggcgctggcg
      961 gggggcgatc tgccggtaca aatcttaagc gatgtgatcc tgctgcgctc ctggtctaat
     1021 caaacagtcg atggtgatat cagcacctgc gtcagcgtgg cggaagattc acaggcgtgg
     1081 ccgctggtca ccagcaccaa cgacaactgt cttggcagcg actgcccgat gtataaagat
     1141 tgctttgtgg tcaaagcacg taaaaaagcg atggacgccg atgtggtggt ggtaaaccat
     1201 catctctttc tggcggatat ggtggttaaa gagagtggat ttggcgaact gatcccggaa
     1261 gcggacgtca tgatcttcga cgaagcccac cagctaccgg acattgccag ccagtatttt
     1321 ggtcagtcac tctccagtcg acaactgctc gacctggcaa aagacatcac catcgcttac
     1381 cgcaccgaat taaaagacac ccagcagtta caaaagtgcg ctgatcgtct tgcccagagt
     1441 gcgcaggatt ttcgtctgca actcggtgag ccaggttatc gcggtaacct gcgtgagctg
     1501 ttagctaatc cgcaaattca gcgggcattt ttactgctcg atgacaccct ggaactttgt
     1561 tatgacgtgg cgaaactgtc actggggcgt tccgccttgc tggatgcggc atttgagcgc
     1621 gctacgttgt atcgcacacg gctgaagcgg ctaaaagaga tcaatcagcc gggctacagc
     1681 tactggtacg aatgcacttc gcgccatttt actctggctc tcacgccgct cagcgtggcg
     1741 gataaattca aagagttaat ggcgcaaaag cctggtagct ggatcttcac ctcagcaacg
     1801 ctgtcggtga acgacgatct gcatcatttc acctcgcggc ttgggataga acaggcggag
     1861 tcgttgctat tgccaagccc gtttgattac agccgccagg cgttactctg tgtgccgcgc
     1921 aatctgccgc aaaccaacca gccaggttct gctcgccagt tagcggcaat gctgcgaccg
     1981 atcatcgaag ctaacaacgg tcgttgtttt atgctttgta cctcgcacgc catgatgcgt
     2041 gatctggccg agcagttccg cgctaccatg acgcttcccg ttttgttgca gggggaaacc
     2101 agcaaagggc aactgttgca gcaatttgtc agcgccggta acgcgcttct tgtggcaacc
     2161 agtagtttct gggaaggggt ggacgtgcgt ggcgatacat tgtcattggt gattatcgac
     2221 aagttgccgt ttacctcgcc ggatgatcca ctgttaaaag cgcgcatgga agattgtcgt
     2281 ttgcgcggtg gcgacccgtt cgatgaagtg caactaccag atgccgtcat tactctcaaa
     2341 cagggggtag ggcgactgat tcgcgacgcc gacgatcgtg gcgtgctggt gatttgtgac
     2401 aatcggctgg tgatgcgtcc ttacggcgcg acgtttctcg ccagtctgcc gcccgcgcca
     2461 cgcactcgtg acattgcccg tgcggttcgt ttccttgcga taccatcctc caggtaattt
     2521 gttacggagt atggtatgat gcgcgccttt ttattgatct aagcacgaag acccatgcga
     2581 attctggcta tcgataccgc gacagaggcc tgctctgtcg ccctgtggaa cgacggtact
     2641 gtcaacgctc attttgagct ttgccctcgt gaacatactc aacgaatctt accgatggtg
     2701 caggatatcc tgaccaccag cggaacttcc ctgactgata ttaacgctct ggcttacggg
     2761 cgcggccccg gtagctttac cggcgtgcgc attggtattg gcattgcgca agggctggca
     2821 cttggcgcag agctaccgat gattggcgtc tccacgctaa tgacgatggc gcaaggggcg
     2881 tggcgcaaaa acggcgcaac ccgcgtgctg gcagccattg acgcgcgaat gggcgaagtt
     2941 tactgggccg aatatcagcg tgatgaaaac ggtatctggc gcggtgaaga aaccgaagcc
     3001 gtactcaaac ccgaactcgt tcatgaacga atgcaacagc tttccggcga atgggtaacg
     3061 gtagggacgg gctggcaagc ctggccggat ctcggtaaag agagcgggct ggttttgcgc
     3121 gacggagaag ttttactgcc agcggcggaa gatatgctgc cgattgcgtg tcagatgttt
     3181 gccgagggta aaacggtggc ggtggaacat gccgaaccgg tttatttacg taacaacgtc
     3241 gcatggaaga aacttccggg caaagaatga atcttaagag tatgccctga gaaaaaaagg
     3301 agtcgcatca tggcggttca aaagaatgtt atcaaaggca tactggcagg tacgtttgcg
     3361 ctaatgctga gcggttgtgt cactgtgccg gacgccatta aaggcagcag tcccacgccg
     3421 caacaagatt tagttcgggt gatgagtgcg ccgcagctgt acgttggtca ggaggcacgc
     3481 tttggtggca aagtggttgc ggtacaaaac cagcaaggga aaacccgcct ggaaattgct
     3541 accgtaccgc tggacagcgg agcaagaccg acgctgggag aaccttctcg cggtcgcatt
     3601 tatgccgata tgaacggttt tctggacccg gtggatttcc gtggacaact ggttacggta
     3661 gtcgggccaa tcactggtgc ggttgacgga aaaatcggca acacgcccta taaatttatg
     3721 gtgatgcaag taacgggtta caaacgttgg catttaaccc agcaggtgat tatgccgcct
     3781 cagccgattg atccatggtt ttatggcggt cgtggctggc cctatggcta tggcggatgg
     3841 ggctggtata atcccggccc cgcgagagta caaacagttg taactgaata attgcttgtt
     3901 tttaaagaaa aagaaacagc ggctggtccg ctgtttctgc attcttacgg taaagataaa
     3961 aataaatagt gacgcgcttc gcaacctttt cgttgggtaa ttatcaagct ggtatgatga
     4021 gttaatatta tgttaacggc atgtatatca tttggggttg cgatgacgac gaacacgcat
     4081 tttagaggtg aagaattgaa gaaggtttgg cttaaccgtt atcccgcgga cgttccgacg
     4141 gagatcaacc ctgaccgtta tcaatctctg gtagatatgt ttgagcagtc ggtcgcgcgc
     4201 tacgccgatc agcctgcgtt tgtgaatatg ggggaggtaa tgaccttccg caagctggaa
     4261 gaacgcagtc gcgcgtttgc cgcttatttg caacaagggt tggggctgaa gaaaggcgat
     4321 cgcgttgcgt tgatgatgcc taatttattg caatatccgg tggcgctgtt tggcattttg
     4381 cgtgccggga tgatcgtcgt aaacgttaac ccgttgtata ccccgcgtga gcttgagcat
     4441 cagcttaacg atagcggcgc atcggcgatt gttatcgtgt ctaactttgc tcacacactg
     4501 gaaaaagtgg ttgataaaac cgccgttcag cacgtaattc tgacccgtat gggcgatcag
     4561 ctatctacgg caaaaggcac ggtagtcaat ttcgttgtta aatacatcaa gcgtttggtg
     4621 ccgaaatacc atctgccaga tgccatttca tttcgtagcg cactgcataa cggctaccgg
     4681 atgcagtacg tcaaacccga actggtgccg gaagatttag ccttcctgca atacaccggc
     4741 ggcaccacag gtgtggcgaa aggcgcgatg ctgactcacc gcaatatgct ggcgaacctg
     4801 gaacaggtca acgccaccta tgggccgttg ctgcatccag gtaaagaact ggtagtaacg
     4861 gcactgccgc tgtatcacat ttttgccctg accattaact gcctgctgtt tatcgaactg
     4921 ggtgggcaga acctgcttat cactaacccg cgcgatattc cagggttggt aaaagagtta
     4981 gcgaaatatc cgtttaccgc tatcacgggc gttaacacct tgttcaatgc gttgctgaac
     5041 aataaagagt tccagcagct ggatttctcc agtctgcatc tttccgcagg cggagggatg
     5101 ccagtgcagc aagtggtggc agagcgttgg gtgaaactga caggacagta tctgctggaa
     5161 ggctatggcc ttaccgagtg tgcgccgctg gtcagcgtta acccatatga tattgattat
     5221 catagtggta gcatcggttt gccggtgccg tcgacggaag ccaaactggt ggatgatgat
     5281 gataatgaag taccaccggg tcaaccgggt gagctttgtg tcaaaggacc gcaggtgatg
     5341 ctgggttact ggcagcgtcc ggatgctaca gatgagatca tcaaaaatgg ctggttacac
     5401 accggcgaca tcgcggtgat ggatgaagaa gggttcctgc gcattgtcga tcgtaaaaaa
     5461 gacatgattc tggtttccgg ttttaacgtc tatcccaacg agattgaaga tgtcgtcatg
     5521 cagcatcctg gcgtacagga agtcgcggct gttggcgtac cttccggctc cagtggtgaa
     5581 gcggtgaaaa tcttcgtagt gaaaaaagat ccatcgctta ccgaagagtc actggtgacc
     5641 ttttgccgcc gtcagctcac gggctacaaa gtaccgaagc tggtggagtt tcgtgatgag
     5701 ttaccgaaat ctaacgtcgg aaaaattttg cgacgagaat tacgtgacga agcgcgcggc
     5761 aaagtggaca ataaagcctg agtgttaagt cagtcgtcag acgccggtta atccggcgtt
     5821 ttttttgacg cccactaaag agaaaacaat ttgaattacc aaatgattac cacggacgat
     5881 gcgctggctt ctttgtgtga agccgtccgt gcctttccgg cgatagccct ggatactgaa
     5941 tttgttcgta cgcgcactta ttacccgcag ctggggttga ttcaactttt cgatggcgag
     6001 catctggcgc taattgatcc tctcgggatc accgactggt caccgctgaa agcgatcctg
     6061 cgcgatccgt ccatcacaaa atttctccat gcaggcagtg aagatctgga agtgttcctc
     6121 aatgtctttg gcgaattacc acaacccttg attgacacgc aaatccttgc tgccttctgc
     6181 ggacgcccga tgtcatgggg tttcgcttcc atggtggaag agtattccgg cgttacgctg
     6241 gacaagagtg aatcgcgcac cgactggctg gccagaccgc tgaccgaacg tcagtgtgaa
     6301 tacgcagcgg cggatgtctg gtatctgtta ccgatcaccg ccaagcttat ggtagaaacg
     6361 gaggcctccg gctggctacc tgcggcgctg gatgaatgcc gcctgatgca aatgcgtcgt
     6421 caggaagtcg ttgcgccgga agatgcctgg cgtgatatca ccaatgcctg gcagttacgc
     6481 acacgccaac tggcctgtct gcaactgtta gccgactggc gactgcgcaa ggcgcgagag
     6541 cgtgatctgg cggtgaactt tgtcgtgcgt gaagagcatt tgtggtcggt cgcgcgttat
     6601 atgccgggaa gtttaggcga actggacagc ctgggtttat ccggtagcga aatccgcttt
     6661 cacggtaaaa cgctgctggc gctggtggaa aaagcgcaga cattgccgga agaggcctta
     6721 ccgcagccga tgcttaacct gatggacatg cctggttatc gtaaagtgtt taaagcgatt
     6781 aagtcgctga ttactgacgt gagcgaaacg cataagatca gcgccgaatt gctggcatcg
     6841 cgtcggcaaa tcaaccaact gctgaactgg cactggaaac tgaaaccgca gaacaatttg
     6901 ccggagctga tttccggctg gcgtggtgag ctgatggcgg aagcattaca caatttattg
     6961 caggaatatc cgcagtaaaa tcttccgaag ccggactggg cgcgctcagc gccacatccg
     7021 gctttggcaa actacaaatc caacaccagg cgtttaccct tcgcacgcga acaacagatc
     7081 aacatacttt gctggctggc acgctcttca tcgctaaaat actgatcccg atggtcagct
     7141 tcaccttcca gtattgctgt ttcgcaggtt ccgcataccc cttcacgaca taaacattcc
     7201 actttcgcgg ctttattatt ttcaataacc tgcaaaatag tcatctcttc cggcaccaca
     7261 aactcttttc cggaacgggc aagcaccagg gtaaaggcat cgccggtttt gtcttcgata
     7321 gcaaattgct caaagtgcag cgtatcggcg acgatgtcca gacgcgcagc ttcacttctt
     7381 accgcttcat ttagcgcctc gggaccacag gtataaacgt gtgtgccagg ttcgatatcc
     7441 gccaataatc gcgctaattc cagtcgtgtt ccggttgatg agtgatgcaa gtgaactttc
     7501 tcagcctgcg gatgctggac tagctcatca cgaaatgcgc aactttctgg atttcgcgag
     7561 cagtaatgta gctgccagtc gacgtcgctg tgttgcagct ctgccatgtg cgacaggaaa
     7621 ggggtgatac caataccgcc cgcgataaac agatgctttc tggctgaggg aattagcgca
     7681 aacaggttat taggcgttga aatcgttaac cgatcgccca cttttacctg ctgatgcaaa
     7741 aagcgggaac cgccgcgcga gttttcctcc agccgaacgg caatctgata acaagaggtg
     7801 tcatgcggcg aactcagtag tgaatacgca ttgctgtaat ggttatcacc atcgctcatc
     7861 tgcacaatga tgtgacttcc tccggtgaac gcaggcagcg gtttgccatc ggttgccacc
     7921 agcgtgaagc gtttcacctg ttcggtaagc ggttccacct gactcacctg tacttcaaac
     7981 atttgatagt ctgacattgc tgtactccat caaaaatcag cccagcggcg gaaaataccg
     8041 ccgccgatgt cattagtcct taaacacctg cgccagcaga ttatggaaat gggcgatacc
     8101 atgttcggaa atgccactac cgctactgtc ggccatgatg cgcccctgac cacgatagcc
     8161 acgcgatttc agccctttct gtacgctttc aaccagacgt aaatcttccg gacggaacac
     8221 atcgcgatac cactcaatca gcgatttttg ctcgtcggtt aactcttcat tggtgaagta
     8281 aatatcgtag ttttgcaggg tagtttcaga atccaccggg aattcataaa tgaccgtcat
     8341 catccctttg atcggggtga cgttcagcat cgtgcacggc cacagccaga aaccgtggaa
     8401 tgccgcatcc gtaccctctt caaatttaaa cgactgttcg gacggtttgg cgaaaccgta
     8461 ttgcagcgtc cagttaccgt gcatggtgtg ccagtaacga tcaacctgta cggagtcgga
     8521 gaaacctgga tgcgccggac cacagtgata gcactcgaga tagttatcga caatgttctt
     8581 ccagttggca ggcgtgcggg tggtaaagcg ggccgccagt ttcagatctt ggacttccgg
     8641 gcaggcttcc agcactttcg cgcccagacc gggtaactga tcttctacgc tggtggcgtt
     8701 ggggtccatg ttgatgaaga cgaatccggc atattcttcc agacgcaccg gaaccagttg
     8761 cgctttgtcg ctatcgaaat tggcgacgtt ttcgcagtta cgtgcatggg ccagattgcc
     8821 atcgagtttg aatgcccatg cgtgatacgg gcaggtaatc acattttttg cttttccttc
     8881 accgctcaac aactgatgac cacggtgcgg acacacgtta tagaacgcgc gcaaaacctt
     8941 atcacgaccg cgtaccagca cgatgctttc gccaatgatc tcacgcgtca cataatcatt
     9001 ggcattcgcc agttcgctgc tgtgagcgac gcaaatccag cttttggcga agacgttctc
     9061 tttttcgtgt tcaaacgcgt tctgatcggt ataaaaacgg gcaggaatgg tccacgcctc
     9121 ttgcgggtta gcgcaaaaat tttcgggtag tacaaagtca gggctcagat tgctcatagg
     9181 gataccttta tcgttaggat gatggcgctc agttgtcgcc tttgaatacg gttcccgcag
     9241 ggagcactgg cgttttttcc agggcttcaa ccggtgtctg cgggagataa tgttcaactt
     9301 gatgagccgg aatgtcggcg taatcctgtt tcagccagcg aataaatccg ccgactttca
     9361 ccagtaaaat gacgaggaag ggaagggctg tgagcacgac ggtggttttc atcgtttcca
     9421 gcgaagcacc ggtaaacaag atggaaagcg ggatcagagt gatcaccacg caccagaaaa
     9481 gacgcagccc acggtcagga tcgtcacctt cctggagatt acgcgtactg gtcgcagcca
     9541 tggtgtaggc caccgcatcc atatgcgagg caaggaaaat aatcatcacg cccaggtatg
     9601 cggcgaggaa caatttaccg gctggcaacg acatcagaac ttgctgtaca gctgtttcgc
     9661 cgcccagtgt ttccagcacc tgtgggacgt tgattacgcc attgataaac tgatgaatgg
     9721 catagctttc cattacgccg aagaagaacc agcaaccgac ggtgctgccg aggatcagtc
     9781 cccagataac ttctttaatc ttacgaccgc gggaaacgcg ggtgacaaac attgctacgc
     9841 caggggtgta tgagatccac cacagccagt agaaaacggt ccagttgcgg gtaaatgaac
     9901 catcgccaag cggatcggtg aataagctca tttgcaggaa gttttgcgtg gtcagaccga
     9961 tggcattgat gatgctgttg gtaataaatt cggttgggcc gacaatcagc accagtaatg
    10021 gcagcaggaa cgcgccccag ccaaccattt tgctcagacg ttgcaaaccg ttgttgatac
    10081 caatccacga gcttaggcaa aaaatgccgc cggaaagcag gatcacaaat gcctgcacgg
    10141 tgaagttatc gggtaaaccg gtcagcgcgg aaagtccacg agtaaaggtt gctgcggtga
    10201 caacaaggga aatagtcagt gcgccgacag tggcgatcag gaacatcaaa tcaaccagtt
    10261 ttccccatgg gccttgcggg cgaacgccgg taatcgcggc aataatgccg gaaagactca
    10321 gacctttgtt tttacgcaca tgaaagtgat aagccatgat taatgaggcc agcgtataag
    10381 ttgcccaggc gctgatgccc cagtggaaga aggagtaagg gacgctaaat tcgagtgcct
    10441 gttgtgaacg cggtgcgata tttaatccag gcgtttgata ataataggcc cattcagcaa
    10501 ccccccaata aagcgtagaa gaacctaaac cggcacaaat aaacataaac agccaggaga
    10561 gcgtgctgta ttccggtttt ccttcgccaa gacgaatatt gccgtattta ctggtggcta
    10621 aataaatcac cagtcccatt gccagcaaaa ccaatacctg tacggcggaa cctaacgtac
    10681 gcgtaacaaa agtgtatatt gcgttggccg cgtcggccga ttgttgtggg aaaaggctta
    10741 atgcagctgc gatgaaaaga atggccacca ggctgatgct tatcagcggc acatcttttt
    10801 tcttaacatt gctcatcatg agtcatctcc tgggcataaa agcccgtctc tggcttttgc
    10861 cagttgtaat tatttgcagg gtaatgagtt gtatgttgtg tttaacaaat tgattatttg
    10921 tatgttacat aaattaaaca attaagaata cggaaagcca taaaacacga aacggaaaca
    10981 ataaaaggtg aaatgacgca ggttatttac tgcaaagaac gaataaaaaa accgcgtcag
    11041 tgacgcggtt caaataaact ggtttgacct taacgcaaaa taattttgca aatcgcgtcg
    11101 gcaacctgtg gcgtggtggc attgcctttc atatcaggtg ttttcggccc gtgagcaatc
    11161 acttcttcaa ttgctgccag aataccgtta tgcgcttgct ggaaacgctc atcgccattg
    11221 ccgagaaaat cgagcatcat tgcaccggcc caaatcgtgg cgatagggtt agcaatgttt
    11281 ttcccgtaga tatccggcgc ggaaccgtgg actggctcga agagcgacgg gaaagtgcgt
    11341 tccggattca ggttggcgga tggggcaatg ccaatggtgc cggtgcaggc cgggccaaga
    11401 tcggaaagga tatcgccaaa taaattggac gccaccacca catcgaagcg ttccggctgc
    11461 atcacaaaac gcgcgcagag aatatcaata tgctgcttgt cccagcggat ctccgggtaa
    11521 ttctcggcca ttgcttccac tcgctcatcc cagtacggca tgctgatggc taaaccgttt
    11581 gatttagtgg cagaagttag cgtcttacgt gggcggcttt gcgcaagttc gaaggcataa
    11641 cgcaaaatgc gatcgacacc acgacgcgta aataccgatt cctgaatgac gacttcatgc
    11701 tctgtacctt cattcactct accgccgagc gaggaatatt cgccttcggt gttttccctg
    11761 accacgtaaa aatcgatgtc gccaggctgt tttcccgcca gcgggcaggg aacgccagga
    11821 aagagacgaa ccgggcgcag gttgacgtac tggtcgaatt cacgacgaaa tttcagcagc
    11881 gaaccccaca acgaaatatg gtcaggaacg gtgtccggcc agccgacggc accaaaatag
    11941 atggcgtcga aacggctaag ttgctcatgc cagtcgtccg gcatcatttt accgtgatgg
    12001 ctgtaatact cgcagctcgc ccactccatt tgctcaaaac tcaaggcgaa gccccagcgc
    12061 tcagcggcag cctgtaacac gcgaatccct tcaggaagga cttctttgcc aatcccgtct
    12121 cccgggatcg cagcaatacg catcgttttc atcattgggt tctcacttcc tgaccatcaa
    12181 aattgacaac atcaccatcc tataattgac tgaaaaggaa ttaatccccg taactggtga
    12241 aacataaaac acgaatcatg aataatttac cgctgctgaa tgatttgcgc gtctttatgc
    12301 tggtggctcg tcgggctggt tttgccgccg tggcggaaga actgggcgtt tcaccggcgt
    12361 tcgtcagcaa gcgcatcgcc ttgctggagc aaacgctaaa cgtggtgttg ttacaccgca
    12421 ccacccgccg cgtcaccatt accgaagaag gagagcggat ttatgaatgg gcgcagcgga
    12481 ttttgcagga tgtcggtcag atgatggacg aactttctga cgtgcgtcag gtgccgcagg
    12541 ggatgctgcg tattatcagc agctttggtt ttggacgaca ggtggtggct ccggcgttat
    12601 cagcgctggc taaggcatat ccgcaactgg aattacgttt tgatgtggaa gaccggttgg
    12661 tcgatttggt caatgaaggt gttgatctcg atattcgcat tggcgatgac atcgccccta
    12721 atttaatcgc ccgcaaactg gcgactaatt accgcattct ctgcgcgtcg ccggaattta
    12781 ttgcgcaaca tggcgcgccg aaacatttaa ccgatctttc cgcattgccg tgcctggtca
    12841 ttaaagagcg cgatcatcct tttggtgtct ggcaattacg caataaagaa ggccaccatg
    12901 cgattaaagt caccggccca ctgtcgtcta atcatggaga aattgtccat cagtggtgtc
    12961 tcgatgggca ggggattgct ctgcgctcct ggtgggatgt tagcgaaaac attgccagtg
    13021 gtcatttagt gcaagtgcta ccggaatatt accagccagc gaacgtctgg gccgtttatg
    13081 tttcaaggct ggcgacgtca gcgaaagtgc ggataacggt agaattttta cgccagtatt
    13141 ttgccgagca ctacccgaat ttttcactgg agcatgcctg attaatgatt caattatcgg
    13201 gttgatatca ggttaaaacc tgattttctc ctttctaagc cgctacagat tggttagcat
    13261 attcaccttt aatcgcgcat gatcgaaaga taattaaaga ggttaatgtg ttcgctgaat
    13321 acggggttct gaattactgg acctatctgg ttggggccat ttttattgtg ttggtgccag
    13381 ggccaaatac tctgtttgta ctcaaaaata gcgtcagtag cggtatgaaa ggcggttatc
    13441 ttgcggcctg cggtgtattt attggcgatg cggtattgat gtttctggca tgggctggag
    13501 tggcgacatt aattaagacc accccgatat tattcaacat tgtacgttat cttggtgcgt
    13561 tttatttgct ctatctgggg agtaaaattc tttacgcgac cctgaagggt aaaaatagcg
    13621 aggccaaatc cgatgagccc caatacggtg ctatttttaa acgcgcgtta attttgagcc
    13681 tgactaatcc gaaagccatt ttgttctatg tgtcgttttt cgtacagttt atcgatgtta
    13741 atgccccaca tacgggaatt tcattcttta ttctggcgac gacgctggaa ctggtgagtt
    13801 tctgctattt gagcttcctg attatttctg gtgcttttgt cacgcagtac atacgtacca
    13861 aaaagaaact ggctaaagtg ggaaactcac tgattggttt gatgttcgtg ggtttcgctg
    13921 cccgactggc gacgctgcaa tcctgataca ttcagcccgc gttgtcgcgg gcttcccact
    13981 tgtaatcctc cctgattttt ctctgatatg gtgctaaaaa gtaaccaata aatggtattt
    14041 aaaatgcaaa ttatcaggcg taccctgaaa cggctggaat aaaccgtttt cagcgcattc
    14101 accgaaggag ggaaaaggat gctgcaaatc ccacagaatt atattcatac gcgctcaacg
    14161 cctttctgga ataaacaaac tgcgcctgcc ggaatattcg aacgtcatct tgataaagga
    14221 acgcgcccgg gggtttaccc acgcctttcc gttatgcatg gggcggtcaa atatttcggc
    14281 taccctgatg aacacagtgc agagcctgat caggtgatcc ttatcgaagc ggggcagttt
    14341 gcggtgttcc ctccagaaaa gtggcacaac attgaagcca tgactgacga tacttatttc
    14401 aacattgact tcttcgtggc tcctgaagtc ctgatggaag gtgcgcaaca acggaaagtc
    14461 attcataacg ggaaatgaat catgggcaaa gcaacgtata ccgtgaccgt caccaataac
    14521 agcaatggcg tttctgtcga ttatgaaaca gagacgccga tgactttgct ggtgccagaa
    14581 gtggcggctg aagtgataaa agatctggtg aataccgtac gttcttatga cacggaaaac
    14641 gaacatgatg tttgtggttg gtaattatca ctgatcgttg atggcgcaat ttctgcgcca
    14701 tcgcaaaaaa aacctccgtg atgctcgtca caaatcttgc cctcccgatg ttgtgctatg
    14761 ctttttatca gcgactaacc cgctaatgca aaacagataa gtgaggaaac aatgggcatt
    14821 ctgtcatgga ttatttttgg gcttattgcc ggtattctgg cgaagtggat catgccaggt
    14881 aaagatggcg gtggattctt tatgactatc ctgctgggga tagtcggtgc cgtagtcggc
    14941 ggatggatca gcacgctgtt tggctttggt aaagtcgatg gcttcaattt tggcagcttc
    15001 gtggttgccg ttattggtgc gattgtcgtg ctatttatct acaggaagat taaaagttaa
    15061 cgcttaaatt gcacaaaggc tgcacacagg cagcctttgc tattttttag aggtgacgtt
    15121 accaccatcc cagctcagtg ccgagtacga caataatcgc cacacccatc ataataatcg
    15181 ttgttttttt catctttatg ctcccggggc agcatagccg ccaataaaaa accatgctaa
    15241 aaatacgacc gctgtaatga agattacaac cggaaaaata ataccgattc gcatgttatc
    15301 accaatcaaa aggatgtgaa tacgcctcag gaatgtagcg ctggatgcgc gacaaaaggt
    15361 gttttctgtt tgtgtttttt ctcctgatac atcgcaatat cggcagcatg cagggcactg
    15421 tctgcatcgg ttgtttcagg atcgacttct actatgccaa gactggcacc gggataatac
    15481 aaatcaacat cacctaagtg atattctcca cgtatttgct gctgaatgcg ttctcgcagc
    15541 gacgaaatat ccgcattctc gttgtttagt gaaacaacta aaaactcatc accgccgaga
    15601 cgaccaataa cttcgccatt ttgctggagc gtattaaggc gctcgccaac ctgaatgaga
    15661 aacagatcgc cactattatg accaaaacga tcattgatta atttgaagtt atccagatcg
    15721 ataaacgcga tcattatctt atggttaaga tgccgggcga gggaaaacag tgtcgtcaga
    15781 ttttcaaaaa tcgcccgccg attcggtagc ccggttaacg agtcggtata cgattgcgca
    15841 atcagcgcag cattggcttc gcgcagttgt tcaaccagtg cctctttttg aatatattgt
    15901 gcaatcagtc cggcgaataa ctgtaaaacc tgttccgcgc gttcactcca ctggcgcttc
    15961 tcactgctgg cggcgcaaag cgtgccatag aatgatccat ccggtaagtg aattggcgtg
    16021 ctcagaaatg tggtgatgcc aagattgcgt gccgcaatac agtcacccca acggtcgggg
    16081 acttcatcgc tgaaaaagca gttttcatca atggcgcgtt tgcataacga gtaatcccac
    16141 gagacggtaa aattctccgg gatgtgcatt ttctggctgt tacgggcaaa cataatatgc
    16201 tgcaggcgcg cttcgacatc cactttggtc aggtaggttg attccatatc agtgaccatt
    16261 tccagcatct ccagaagctg tcggaccaga ctttccagtg actgttcttt ggcaagggat
    16321 tgcgagacgc gggcgataat ctgatctgac acggaacaac tccaggatac ggcgggtaaa
    16381 tgctgattat ttaatctttg caggcatgat aaaacatcat caacaaagat gaaacaagac
    16441 gtaatatgtt ttgagtgaat gtaataaaaa tagtgctgca aaacaaaacg ataccgcgat
    16501 actctaatct tggataaata tgtgagatca ataaatgaaa attatcagtt ttgttctgcc
    16561 ttgcttgctg gtcctggctg gttgttcaac cccttctcag ccagaagcac ctaaaccgcc
    16621 gcagattggt atggcaaatc cggcgtcggt ctattgccag cagaagggcg ggacgctcat
    16681 tccagtgcag acagcgcaag gggtcagcaa caattgcaaa ttaccgggcg gtgaaacgat
    16741 tgatgaatgg gcattgtggc ggcgtgacca tccggctggt gaaaaataac cgccggatgc
    16801 tgtactaaaa tcacaagcta cgtagccagt cggccagcac cagcgcatgg ttctgcgtgg
    16861 tgttttttgc tgcgtagagc agggtcagcg gctgtttttt ggcgatgtcc gccagccgct
    16921 ttccttcttg ctcgtgttgt gccagttctg caagatattg ctcgcgaaag gttgcaaaat
    16981 cgacgacttc gccgtgaaag gctttgcgca gttccgttga cggcgtgatt tctttatccc
    17041 actcatcaag ggctaaatcg gtttttttga taccgcgcgg ccagaggcgg tcgaccagta
    17101 tgcgataacc atcgctctgt tctgccggat cataaacgcg tttgcactga atattcatcg
    17161 tgtttgttct ccctttcacg catgatttgt cacgtcacta tagcaccgga tattagcgaa
    17221 tttctttgtc tcttccggcg caaagtccga aaatcgccat cagtagtgaa agtattgcaa
    17281 cacccattag tggtacggac cagtcaccgt tagcatcgtg tattttaccc atcagcggcg
    17341 gcccacaggc tgccagcaaa tacccgacgg attgtgccat ccccgagagt gccgccgcct
    17401 gatgcgcaga acttgcccga agaccaatga acgtcaaccc cagtatcatt gttgcgccgg
    17461 aaccaaaacc gaaaagcaga gtccaggtga tcgcgtgtgc tggcataaag cagagcccaa
    17521 ccgcgcccac tgcgcacatc aaggcaacga acgctgcaat accacgctga tctttcacat
    17581 gatgtaagaa aagtgggatc agcaaaccgg gagctgctgt ggctagttgc agcaaaccat
    17641 gcagtgaacc cgcctgtgct tcgctatagc cgtgactgat gaggatcgcc ggaagccagc
    17701 caataatcac gtaatagacc agtgagttga tcccaagaaa caatgtgacc tgccaggcaa
    17761 gcggcgaacg ccagataccc cgagtatgta aggcgcgcga ggtactcaaa tttgcatgtt
    17821 gttggcttcg ccactgtggc agccataaaa aaagagccag cagaggaaaa cacatcagca
    17881 tgagcaacgc gccttgccag ccaaaaccgt taaaagccag cggcactacc atagccgatc
    17941 caagtgccgc cgcagctccc attgtcaggg aatatgcgcc ggtaagtctg gcgacggaat
    18001 gagggaaatc gcgtttaatt aatcctggca gtaagacatt acctaatgca atcccaccac
    18061 caatgaccgc tgtaccgcca aataataagt aaggcgaagg gagagagcga attgcgatac
    18121 cagcacagat caaaagtaac gcggcaaaca ggctacgttc cataccaaaa cgtcgcgcta
    18181 ccggggcggc caaaggtgag attagcgcaa aggccaataa tggcagggtg gtcaataagc
    18241 cggtttgcgc tgtcgtcagc gagtaagcgg aacgaatcgt atccagtaac ggtgctgcgc
    18301 cggtaaaggt gacgcgtaat gttgtggcaa tcatcagaat gccagcgata aggacaatcc
    18361 tgtttttgcc gcttaatgaa gttgaacagg tcatatgctt ctcattgtta caaacgaggc
    18421 tgtaccttac cgtttttatt gttatgttga atcaagctaa aatgacaaat tatcgctaaa
    18481 ttcggacaaa atgatgcaca ggttgaatct caatggctat gaacccgacc gacaccatga
    18541 agccgcggtc gcattttgta ttcacgccgg aacggatgag ttaaccagtc ctgtacatca
    18601 gcaccgcaaa ggacagctga tactggcgct acatggtgca attacctgta cggtggaaaa
    18661 tgctttgtgg atggtgccgc cgcaatatgc ggtttggata ccgggcgggg tggaacatag
    18721 caatcaagtg acagctaacg ctgaactctg ttttctgttt atcgaaccca gcgcagtgac
    18781 aatgccgaca acatgttgca cattgaaaat atcgccctta tgccgggaat tgattttaac
    18841 cctggcaaac aggacgacga ctcagcgtgc tgagccgatg acccacagat taattcaggt
    18901 cctgtttgat gaactccctc agcagccaca acaacagctg cacctgcccg tttcttctca
    18961 tcctaaaatc cgcacgatgg tggagatgat ggcgaaaggg cccgtcgagt gggggtcatt
    19021 agggcaatgg gctggctttt ttgcgatgag tgaacgcaat cttgcgcgac tgatagtaaa
    19081 agagacgggg ttgagttttc gccagtggcg tcagcagtta cagctgatta tggcacttca
    19141 gggattagtg aaaggcgata ctgtacagaa agttgctcat acgctggggt atgattcaac
    19201 aacggccttt attactatgt ttaagaaagg cctggggcaa acgccaggcc gatatattgc
    19261 gggattaact actgtttccc cacaatcagc gaaaccagac ccgcggcaat aagcgggccg
    19321 accggtacgc cgcgaaacag cgccacgcct aaaacagtgc ctaccagcag cccggcgacc
    19381 agttgcggct ggctgcccat taaggtcacg ccgcgcccac ccagccagga gacaatcacg
    19441 cctactgcaa ttgccaccag cgatttccag ttcagaaacg agtgaatcaa ggttgagggc
    19501 ggtagcgtcc cgctggcgat gggggccata acgccaatcg tcagaatgat tatcccgata
    19561 cttagcccct gtttttcaat ccacgggaaa aaggtgctta acggtgtgac gcggacaatg
    19621 atcaacacca gaattgaaac ggcgacagtg gtgttatgac tgataaagcc cagcgcagct
    19681 aacccgagca ggatcagcaa agtgacatca aacatcgtgg tttccttgcc aaaaaataat
    19741 aagcctgctt actttacgct tgctgcagga aggaagtaga aaatctttac gttttttgca
    19801 taaaaaatca ggcgaactgt catcaaactg ccatttgtgc acgctaacaa taacgtgaaa
    19861 acagctatct cccgggggca tgctatgaac gatcaaatgt tcgtcgagac actgattatc
    19921 acgtcatcgt tttttgccat tgctgttgtg ctggttttgt ccgttctgct tattgaacgg
    19981 acaggctagt tacgcagtgc ggcggaaatt gagcagttct ggttgcgcaa tgcgcaaata
    20041 atccgtggtt ttcaaaataa cggatttatc cagcatccct gcattgaagg cgatttcatc
    20101 aaatcgttcg aagagtagag ggtcggcaac cagtttgagt tttggatgga agctgaaagg
    20161 ggggatcgcg ccgaagacac agcctgtgag ttcatcgact tccgccgggc tggccaacga
    20221 agcgcgtaat ccgcctatat ggctggcgag ttggctcaga tcggcctgtt gatcggcagc
    20281 gaggatcgcc agaacatgtt gatttacgcc attgcctttg actttacata ccagtgcctt
    20341 agctccctgg cctaaagcgg tgccacgaat ttctgacacc gcttcacatt tacctaccgc
    20401 ttcatgggta acgacgcgga agttagcacc ttcttgtgat aataaagcga ttaaccgctg
    20461 atgcgtcacg cttcctttag ccatttcagt catttcacgc tcctgagatt acaagcaaac
    20521 aaccacagaa ggttatcatg aacggctgtt tttgttctgc ttattgacat ataaacgctc
    20581 atcggaagct ttatacgcat catgtaacgt atcgttttct ttcatcgcgt aaataccgga
    20641 agagaagccg atctctttct gcggcgcgat atgttgcaga cgtttttcga tacgttcagg
    20701 cagttgtgct gcaatttgcg gcgtcgaatc gacaagaatg atgcagaatt catcgccacc
    20761 gagtcggatg gcataatcac ttttacgaat cgattgttta atcgcctgag ctaacaacgt
    20821 aatcgctaaa tccccttcct gatgaccgag ggtgtcattt attagcttta acttgtccat
    20881 gtcaattgca ataaacatca ccgaagaacc ggattgcacc agtttctgca accgctgctc
    20941 cagttcaggg gttaaaattt tgcgattata cagtccagtc atcgcatcac taatattttc
    21001 tcgcgacaca ttttggtata agcggaagtg catccgcacc atattcagca aaattaacgc
    21061 cgtcaaaatc cagaacagaa cgcttttcca cgaagacgtg ataaagtaaa gaatatcaat
    21121 ggataacgag acacgaatgc cgcccggtaa gtcatggacg taactgacat actggaacag
    21181 attatcttcg ctctggttga tgataatgtc gcgccccgaa tcggtatcgg ttagcgtgac
    21241 attgagaaaa cgccagagga gagggcggtc atgagtgtaa aagatattcc gcaaattgtt
    21301 tttgttaata tccagcagca caatcccttt tagctgccct gcaacataca ccggcgtcag
    21361 caaactcatc acgttctgtt tagtacgctg atcctggtaa atgctcgaca atacggtacg
    21421 cccggtaaat accttttcaa tatctttgcg atcgatacca atacttcctg actgcaggaa
    21481 ggaccagtta ttcatcgcaa agtattcaac attaaccggc tcataaaaat agacatagcg
    21541 gtcattgaga tcgagataat agtgaaattt ctcttcggcc atcccaagac ctgaaccgta
    21601 atcatcctgg tggcggttgc cttccactgc ctgatcaaaa gcgggtaata gtgctgcagc
    21661 ttccacaatt gtgtcgcagt cagtggatgc ggtttgtagt gtgccgcgta agcccgggta
    21721 tttatgggca gtcaggttta agccatgggt attattggca gactcaaaag cctcgcagat
    21781 ggcgcgccgc tgttctggcg acacttccga ctgctcagca gcgagagcgc gcatcacatg
    21841 cgcggcaata ctctgattct gatacttatc ataaagaaag gaggagtctg ctttctgcac
    21901 gatatagcgc agataggcac tcattgctct gtcgctggcg acaagttcaa aaataaggaa
    21961 ggaagatgtc agcacaatga cacttgcggc gataaaatgc cggagcattc tatggtgcaa
    22021 tttcatgata aatggcactc cttcggttag tgaaacgaga aaaccagata aagttcgatt
    22081 gcggcctggc acaggggcgc taattatact catctctgcg ccgctgggga ggggaaattt
    22141 aggggtaata gtaataaatt gtatgcaaaa gccacgcaaa aagcatggct ctgaatattt
    22201 ttgcacttaa atatgatgaa taatcacttt gttgcccccc tcgcttttcg cttcgcgtaa
    22261 ggctttatct gcttccgtca tgacaagcga gatattacgc gtgtcacccg ttgaaaaaac
    22321 cgtgccaata ctgatggtca tttgttcagg aacatcatat ctgttactaa agccggtcag
    22381 gagttcgaca tttttacgca tacgttccgc aatgattttt gcctgggcac tattgagttc
    22441 ggtaaatagc aagccaaaca cctcaccctc cagtcgcgct aaaatatcat cggggcggat
    22501 gctttgctgg ataatatcga caattgaaac cagcacttta tcacccacac gatgtcccca
    22561 ttgggcattg atgcgtttga actgattaat atcaaccagc atgacacaga agtccttaac
    22621 aacaggtcgc gacaataatg actctacgct gttgaaaaag tagcgccgat tataaatatt
    22681 agtcagcaca tcatgaactg ccagtttgct ggagagttgc agttcctgaa aaatgttata
    22741 aatcagaaaa gacacaacaa acagtttact ggaaacttca atcccgcgac tgatatacca
    22801 gatggtgtaa ttatactcat ccagagtcag tagaagtaag ttgcagcata gcgatgccag
    22861 acataataat gctattaacg gccatagttc gctggctaat cgtgtacgca tgataataaa
    22921 gaacagtaaa aatgcccaca gacaaaccaa tatttttgta tagttgattc cccaggtcgc
    22981 agtatggttg tcaggacagt agtcgacgac atacaaagaa tagtcagcgt tataactgct
    23041 cagattgtgt gccagaaggg gaaaaactaa aaaaggaatc agcgccagca acaggattcc
    23101 cgtttttttc ttattgttgt caaggatgtt gtcttttcca taacaaaaga gtgccagcga
    23161 ggttaaacaa ataaaactga gttggcggaa aatatagaaa atagaaacat cattcgactt
    23221 tgtctggatc agtgtactgc cgttaattgg tttatggata attacaatgg tttcaactaa
    23281 atagataagg ccacttaaaa atgcaaggct taaaatgagt gttggtaaaa tttgtttgtt
    23341 agtgaa
//
LOCUS       DBANUU010000076        23257 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000076 DBANUU010000000
VERSION     DBANUU010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.076"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..3855)
                     /gene="entF"
                     /locus_tag="ACIE68_002855"
     CDS             complement(<1..3855)
                     /gene="entF"
                     /locus_tag="ACIE68_002855"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFX5909143.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDSPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPDVQAQTHTLATGPVNDLELALFPDVHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGP"
     gene            complement(3852..4070)
                     /gene="ybdZ"
                     /locus_tag="ACIE68_002856"
     CDS             complement(3852..4070)
                     /gene="ybdZ"
                     /locus_tag="ACIE68_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFX5909144.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEAHWRTLTPTNFTQLQEAQ"
     gene            complement(4073..5275)
                     /gene="fes"
                     /locus_tag="ACIE68_002857"
     CDS             complement(4073..5275)
                     /gene="fes"
                     /locus_tag="ACIE68_002857"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFX5909145.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     PIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRGDRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5518..7758
                     /gene="fepA"
                     /locus_tag="ACIE68_002858"
     CDS             5518..7758
                     /gene="fepA"
                     /locus_tag="ACIE68_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFX5909146.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPLDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7933..8553
                     /gene="entD"
                     /locus_tag="ACIE68_002859"
     CDS             7933..8553
                     /gene="entD"
                     /locus_tag="ACIE68_002859"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFX5909147.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8675..8827)
                     /gene="hokE"
                     /locus_tag="ACIE68_002860"
     CDS             complement(8675..8827)
                     /gene="hokE"
                     /locus_tag="ACIE68_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFX5909148.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            8796..9064
                     /locus_tag="ACIE68_002861"
                     /pseudo
     CDS             8796..9064
                     /locus_tag="ACIE68_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9280..10398
                     /locus_tag="ACIE68_002862"
     CDS             9280..10398
                     /locus_tag="ACIE68_002862"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFX5909149.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAATDHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            10464..10712
                     /locus_tag="ACIE68_002863"
     CDS             10464..10712
                     /locus_tag="ACIE68_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFX5909150.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVSTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            10777..11145
                     /locus_tag="ACIE68_002864"
     CDS             10777..11145
                     /locus_tag="ACIE68_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFX5909151.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11239..11891
                     /gene="nfsB"
                     /locus_tag="ACIE68_002865"
                     /pseudo
     CDS             11239..11891
                     /gene="nfsB"
                     /locus_tag="ACIE68_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
     gene            11999..13246
                     /gene="ybdG"
                     /locus_tag="ACIE68_002866"
     CDS             11999..13246
                     /gene="ybdG"
                     /locus_tag="ACIE68_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFX5909152.1"
                     /translation="MQDLISQVEDLAGIEIDHITSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13314..14690)
                     /gene="pheP"
                     /locus_tag="ACIE68_002867"
     CDS             complement(13314..14690)
                     /gene="pheP"
                     /locus_tag="ACIE68_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFX5909153.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(14792..17935)
                     /gene="cusA"
                     /locus_tag="ACIE68_002868"
     CDS             complement(14792..17935)
                     /gene="cusA"
                     /locus_tag="ACIE68_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFX5909154.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQQPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAVPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(17947..19170)
                     /gene="cusB"
                     /locus_tag="ACIE68_002869"
     CDS             complement(17947..19170)
                     /gene="cusB"
                     /locus_tag="ACIE68_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFX5909155.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWVAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(19186..19518)
                     /gene="cusF"
                     /locus_tag="ACIE68_002870"
     CDS             complement(19186..19518)
                     /gene="cusF"
                     /locus_tag="ACIE68_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFX5909156.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGEV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(19542..20924)
                     /gene="cusC"
                     /locus_tag="ACIE68_002871"
     CDS             complement(19542..20924)
                     /gene="cusC"
                     /locus_tag="ACIE68_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFX5909157.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21081..21764
                     /gene="cusR"
                     /locus_tag="ACIE68_002872"
     CDS             21081..21764
                     /gene="cusR"
                     /locus_tag="ACIE68_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFX5909158.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            21754..23202
                     /gene="cusS"
                     /locus_tag="ACIE68_002873"
     CDS             21754..23202
                     /gene="cusS"
                     /locus_tag="ACIE68_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFX5909159.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         5561 a         6358 c         5941 g         5397 t
ORIGIN      
        1 cggcccaatt tttacaaacg cccctggaga gataatatcc acatgcgcac aatcctgacg
       61 atagatatcc agctccgcaa tccacggcga ccaggcacgt tcgggactca taccttcctg
      121 aagtgtgcgc tcagcaacaa ataacgttgc ttttccatca aacggaacgc tatgggcggt
      181 ggttaacaag cgcacagcat cagcgtagtt gccttcaatg gtggtaaaca actccgttga
      241 agtacttccc tgctgtgccg ccaggaaggc ctcgcgttcg cggttaatct ccgccagcac
      301 ttccgggtcc agaccattag cctctttttc ctgccagttt tgcgtctctg gcggccaggt
      361 atccagcaaa ccaagaaatg ccacctgttc gccacgggca cgcagtcgcg ccgcaatacc
      421 ctgcgccagc gtaccgccaa gggaataccc cagcaggtaa taagggccgt gcggttgttg
      481 ctcaagtaac gttgccagat gcgcttcgca gacttcatcc aggtttgtcg ctgtctgcat
      541 gggtccatga gggcgcggcg actgaatgcc aataatcgac cattgtggat cgagataacg
      601 cgagagcacg ctaaactgcc aggcaaaacc ggacgccgga tggaaacaaa acagcgtcgg
      661 gccattacct tcacgcaacg gcagaatggt ttcgaatccc atgcgccggg agctgtcctc
      721 ttcaccatca ataatcgttg ccagtttggc gacggttgac gcgaccatca cctgccccgg
      781 cgtcacctga cgagcaaact gtcgacttaa ctgcgctgct agtttcattg ccagtagcga
      841 atgaccgccc agcgcaaaga aatcagcatc ggcatcctgc acgtcacaac ccagcaacga
      901 cgcgaatgcc gcggcgataa tcgtttcact gcccgctttc ggtgcacgtc ccgacgcttg
      961 tgtcttcagt tcaggcaacg gtaaggcttt gcgatccagc ttgccgttgg cgctaagcgg
     1021 caactgcgga agttgcagca gaacgaccgg caccatatgc ggcggcaatg tttcgcgaag
     1081 ctgtgcctgt aatgcgctgg tatccaacgg cagtcccgat tgcgacacca gatagcccac
     1141 caactgacgc gcatcaccac cggtggctgc cgcctggtta atcacacagg cgtgggtaac
     1201 tgcctgttcg acatccggca gcgcctgcat cacgcgatcg atttcgccca gttcgatacg
     1261 ctgcccgcga atttttagct gatcgtcact gcgcccgagg tactccaccg cgccgttatc
     1321 cagccagcgg gcgacgtctc cggtacgata catccgttca cctggggcaa aaggatcggc
     1381 aataaaacgg ctggcggtca gatcgggtcg tccaagatac ccctgcgcca gttggatgcc
     1441 ggtgagatag agatcacccg ccacacccgg cggcaccgga tgcatcatcg catcaagaat
     1501 acgcagaccc gtattccata ccggataacc aatcggcaca ctgctgccgc gcacctgtgc
     1561 cagttcctcg ccaaaagccg gataccagct gacatctacc gccgcttccg tcgggccata
     1621 caggttatgc aacggcgcgc cggttaactg ttgccattcg cggcataaat cagccggtaa
     1681 ggcctcacca ctacagaaaa cctgtttcaa cgtcgcgcaa ttctggcgag cggtttgcgg
     1741 cgtcagcgat gcaacaaatg ccgccagcat cgacggcaca aagtgcgtgg tcgttacgcc
     1801 atattcggca aagaattgct gcatagcgag cgggtcacgg tgcgcttccg gttcagccat
     1861 caccagtttt gcccctgcga taaacggcca gaaaaactcc cacaccgaga catcaaaact
     1921 gcacggcgtt ttttgggcaa cgacatcttc gcctgtaagc gggtagtgat tttgcatcca
     1981 taacaggcgg ttaacgatag ccgtctgccc gaccattacc cctttcggcc tgccggtgga
     2041 gccagaggta aagatgatat aagccgtgtg gtgcggttgt gaaagttgca gcggcgcact
     2101 gccctggggg gtaagcgggg cgttatagca aaggcttgtt aaattgggaa catcgctaaa
     2161 gcgcggcagt tgatcgtcgg tggtaattaa cagcgacgga cgcgcatctt ccagcatcat
     2221 tttcaggcga tcgtccggat agccggtatc cagcggtagc caggccgcgc cagcttcaac
     2281 tattgcatgg agtgccaggg tcaaaaagac cgagcgcggt agtgccaccg ccacgctgtc
     2341 ccctggttta acgccgcgct cacgcagcag attcgccagc gccaccacct gctcgcgcat
     2401 ttcccgatag ctgaacaggt aacgcgcatc tgccagcgcc ggagcatccg gtgtttttgc
     2461 cgcttgttct gccaccagcg cgctcagcgt ggtttctgga atctcaacct gagtggcgtt
     2521 gatctgcgcc aactgcgcat actcacctgg cagcataata tcgacatcgc cgcacaacag
     2581 cgccggatcc gcggcgaact gggcaatcag cattttcagg cgttcagcat gctggattaa
     2641 cgttggctca tcgtaacgct gtttattggc gaggatctca atactcaaat caccgtgtac
     2701 atccgggaac agggccagtt caaggtcatt aaccggaccg gttgccaggg tatgggtttg
     2761 cgcctgaaca tcaggaatat ccagttggta atcaaatacc ttgatattga gtaccggacc
     2821 aaacagcggt tcatcacctg ccgctcgccc gctgtcacgg acaatttgtt cggcatcgta
     2881 acgttgatga cgacgcattt ttttcagttg tgctgccagt cgggttgcca gttccggcag
     2941 cgtttcttgc gccgcaatgt gaatacccaa cggcaaaacg ttgagcacgg gtccggtagc
     3001 cgtcagcgcc gccgagccca gtcgacgcat aaagataaat ccggcggcgt agtccatacg
     3061 attgcacaat cgccccagcc acaaggctgc cagcgcaagg gctaaatcgg tacgctgcac
     3121 acctgaaagt tgcgtagcca gctggcggaa ttccccgtcg gtaaattcca gtttcaggcg
     3181 cagaatatct gccgaggcgc tgcgccccgg taaaggtgcc ggagaaagtg acgcgggcgg
     3241 cggcagttga cgacgctgtt ctgcccagaa tgccgcatca cgctgccagg cttcgctttc
     3301 gcggtattgc tggtactctt ccactacatc agcgaaaggc gtaaatggcg aagcaggcgt
     3361 tggttcgcca cgcagccatg tgcagtaaat attggcgatc tggcgggtaa tggccgggaa
     3421 actgaagcca tcgaccagca aatggtgata acgctgatac cagtaccagc ggttatccgc
     3481 cacctgtatc agctgatgaa agaccagtgg tttaccgcta tcgacgcgca gatcttgttg
     3541 caaatccgcc tgcattaatg cctgcgcagt accgtgcgga tcaatgttgg ttcgtaggtc
     3601 gataatttct ggcagttcga acgtcagcgc atcatcgacc cactgccaga cttcgccgtt
     3661 atcttccgta aaacgcatcc gcagcgtatc tgcttgcgct agtccggcaa ccaccgcgcg
     3721 ggccagtaat ggcgaatcaa cctctccggt taactcaacg taatgcgcca cgctccaggc
     3781 ggagggtaat tctgacagtt tttctgccat ccagatgccg ggctgtgcgg cgaccaaagg
     3841 taaatgctgg ctcattgtgc ctcctgcaac tgggtaaaat tcgtcggtgt cagagtacgc
     3901 cagtgggctt ccagccactg ctggcaggac gcctgtgact gcggctgaca cacaatgtcc
     3961 cagcctgccg gtaaggcgca ttgttgcggc cacagactga attgcccctg cgcattgcgc
     4021 aaaatgtaaa acgctccctg cggatcatcg aagggattac tgaatgccat attcaactcc
     4081 tgtcatggaa aagtggttgc cagaggtcga ttagcccctg catcaagcca ccgcgccaac
     4141 aaagcgcatc atgtccgccg tcaacctgac gccagaaaat ggattctttt atggggtgta
     4201 attgcgcata cagcgcctga ttggcccgca taatcatcgg ctcgcgaata cccgcttcca
     4261 gcacaatgcg cagaccttcg gcgctaactt caccagcttt aagcttttca agtaacacgc
     4321 cctcttgctg cccgccccga tgcggccacc agtacgatcc tgactggctt aatacacagc
     4381 caaagcgttc aggccagtgc agtccggcat acagcgcgga cagcccacca aaactctgcc
     4441 cggcaaccac ggtgcgatcg ccgcgatcgc taaaaggggc aatagctttc accaggggta
     4501 ataactcttg ctgtactgcc agccagaaat ccgcattaca cggcagttcg tgggcgcggt
     4561 gcgtggtgtc gatagcgtcg atcaacacat acacggcggg aggaagttgc tgacgatggg
     4621 tcagcgaagt cagcacaggc cagacgggca tactttgcgc ccaaaattcg ccatcgagca
     4681 aaactgccag cggacgttct tctgctgtta catcgccggt ggtaaaaatc catacacgac
     4741 gtgaattttt caaccgttca cttttccaga taatttcttt ggctgctatt tctggcgctt
     4801 gcggacaatc ccatcccggt tgcagaggcg cttgcggcat ttcgagtgca gaaacagcgt
     4861 gccctcgccc gcctttccag ctttgtggat tcagcggatc ggctattgcc tggggtaata
     4921 gttttcgcca gccttcacgt aattcgaggc gatcggggga tggtgcagaa aaaatgtcat
     4981 cgcgttcggt gggaataaag cagtagctgc cgcgccagtt ggcattgagt tgtgtcgtcc
     5041 actgccagac atcagtacct gcaattcgct gcatcgactg gggctggctg ttctgatgat
     5101 gatcggtcac accagtgatg tagatccata cgcgctttat cggcgagtat tcttcagaac
     5161 cttggggatc acgccaccag aaagtgacct caaacatttc gtcattcaga cgctgccatt
     5221 ccgggccatg tttcgactgc caccagctct cacttcctac ttttaacgcc gtcacaccat
     5281 aaccccatgt ttactgtgca atttttcatt gattgcagaa atatattgat aatattattg
     5341 ataactattt gcatttgcaa tagcgtaatg gcgcgccgtg ggaagcgcgg acatttatta
     5401 accaactgca ctgcgtgtct ttcaggatca aaggttttcg cggtagcggg atgcgtcgtg
     5461 ttgaggacga ccatgcccga cagttgcaat tcgtggcaaa aatgcaggaa taaaacaatg
     5521 aacaagaaga ttcactccct ggccttgttg gtcaatctgg ggatttatgg ggtagcgcag
     5581 gcacaagagc cgaccgatac tcctgtttca catgacgata ccattgtcgt taccgccgcc
     5641 gagcagaact tgcaggcacc tggcgtttcg accatcaccg cagatgaaat ccgcaaaaac
     5701 cccgttgccc gcgatgtgtc ggagatcatc cgtaccatgc caggcgttaa cctgaccggt
     5761 aactccacca gtggtcagcg tgggaataac cgacagattg atattcgcgg tatgggtccg
     5821 gaaaacacgc tgattttgat tgacggcaag ccggtaagca gccgtaactc ggtgcgtcag
     5881 ggctggcgtg gcgagcgcga tacccgtggt gatacctcct gggtgccacc tgaaatgatt
     5941 gaacgtattg aagttctgcg tggtccggca gctgcgcgtt atggcaacgg cgcggcgggc
     6001 ggcgtggtta acatcattac caaaaaaggc agcggtgagt ggcacggctc ctgggacgct
     6061 tatttcaatg cgccagaaca taaagaggaa ggtgccacta aacgcaccaa cttcagcctg
     6121 accggtccgc tgggcgacga attcagcttc cgcttgtatg gcaacctcga caaaacccag
     6181 gctgacgcgt gggatatcaa ccagggccat cagtccgcgc gtgccggaac gtatgccacg
     6241 acgttaccag ccgggcgcga aggggtgatc aacaaagata ttaatggcgt ggtgcgctgg
     6301 gacttcgcgc ctctgcaatc actcgaactg gaagcgggtt acagccgcca gggtaacctg
     6361 tatgcgggcg atacccagaa caccaactct gacgcttata ctcgctcgaa atatggcgat
     6421 gaaaccaacc gcctgtatcg ccagaactac tcgctgacct ggaacggtgg ctgggataac
     6481 ggcgtgacca ccagcaactg ggtgcagtac gaacacaccc gtaactcgcg tattccggaa
     6541 ggtctggcgg gcggtaccga agggaaattt aacgaaaaag cgacacagga tttcgtcgat
     6601 atcgatcttg atgacgtgat gctgcacagt gaagttaacc tgccgcttga tttcctcgtt
     6661 aaccagacgc tgacgctggg tacggagtgg aatcagcaac ggatgaaaga cttgagttcc
     6721 aatacccagg cgctgaccgg gacgaacacc ggtggcgcta ttgatggcgt gagtgccacc
     6781 gaccgtagcc cgtattcaaa agcagaaatt ttctcgctgt ttgccgaaaa caacatggag
     6841 ctgactgaca gcaccatcgt aacgccgggg ctgcgtttcg atcatcacag tattgtcggc
     6901 aataactgga gcccggcgct gaacatatcg caaggtttag gcgatgactt cacgctgaaa
     6961 atgggcatcg cccgtgctta taaagcgccg agcctgtacc agactaaccc gaactacatt
     7021 ctctacagta aaggtcaggg ttgctatgcc agcgcgggcg gctgctatct gcaaggtaac
     7081 gatgacctga aagcagaaac cagcatcaac aaagagattg gtctggagtt caaacgcgac
     7141 ggctggctgg cgggcgtcac ctggttccgt aacgattatc gcaataagat tgaagcgggc
     7201 tatgtggctg tagggcaaaa cgcagtcggc accgatctct atcagtggga taacgtgccg
     7261 aaagcggtgg ttgaaggtct ggaaggatcg ttaaacgtac cggttagcga aacggtgatg
     7321 tggaccaata acatcactta tatgctgaag agtgaaaaca aaaccacggg cgaccgtttg
     7381 tcgatcatcc cggagtatac gttgaactca acgctgagct ggcaggcacg ggaagatttg
     7441 tcgatgcaaa cgaccttcac ctggtacggc aagcagcagc cgaagaagta caactataaa
     7501 ggtcagccag cggttggacc ggaaaccaaa gaaattagtc cttacagcat tgttggcctg
     7561 agcgcgacct gggatgtgac gaagaatgtc agtctgaccg gcggcgtgga caatctgttc
     7621 gacaaacgtt tgtggcgtgc gggtaatgcc cagaccacgg gcgatttggc aggggccaac
     7681 tatatcgccg gtgccggggc gtatacctat aacgagccgg gacgtacgtg gtatatgagc
     7741 gtaaacaccc acttctgatg ctaacgtcag attgttgaca aagtgcgcgt cgttcatgcc
     7801 ggatgcggcg tgaacgcctt atccggccta caaaaatctt gccaattcaa tatattgcag
     7861 gaccatgttg gcctgataag cgtagcgcat caggcagttt tgcgtttgtc atcagtctcg
     7921 aatatggtcg atatgaaaac tacgcatacc tccctcccct ttgccggaca tacgctgcat
     7981 tttgttgagt tcgatccggc gaatttttgt gagcaggatt tactctggct gccgcactac
     8041 gcacaactgc aacacgctgg acgtaaacgt aaaacagagc atttagccgg acggatcgct
     8101 gctgtttatg ctttgcggga atatggctat aaatgtgtgc ccgcaatcgg cgagctacgc
     8161 caacctgtct ggcctgcgga ggtatacggc agtattagcc actgtgggac tacggcatta
     8221 gccgtggtat ctcgtcaacc gattggcatt gatatagaag aaattttttc tgtacaaacc
     8281 gcaagagaat tgacagacaa cattattaca ccagcggaac acgagcgact cgcagactgc
     8341 ggtttagcct tttctctggc gctgacactg gcattttccg ccaaagagag cgcatttaag
     8401 gcaagtgaga tccaaactga tgcaggtttt ctggactatc agataattag ctggaataaa
     8461 cagcaggtca tcattcatcg tgagaatgag atgtttgctg tgcactggca gataaaagaa
     8521 aagatagtca taacgctgtg ccaacacgat taattgacaa catctggtac gattcgcccg
     8581 cagccatcac tgaccacggg cgaaagtgta aagcaggtgc cttaccatcc tgacctgaca
     8641 accggatatg cggggattgc tccccgcaca acggctactt cttcggttcg taagcgagaa
     8701 cagccttaaa ctcaatatta cgttccttta cggtgaactc acacagcgaa tctccgacca
     8761 gaagcgtaaa tcccagcacc gttaaacaca gcactatgac tgccgcaagg gcatatttcg
     8821 tcagcatgtt tatattgcct ccatgcgaag aagggactac catccaaatt gttcgggttt
     8881 ggagtggcag ccccagggtt gatagaaata tctcctgggg cttcatcttt ccagacctca
     8941 gagtaaacct gaaaccggaa agcttcaggc acccgccgtt atcttaccct tcctcaacaa
     9001 aatagctgtt tatttataca gtgagtcctg tttcggactt ctcactcctc atttaatcaa
     9061 ctgaagctga acccgttttt ttctgcatga ctcaatcgtc aaaattcttc gccaccagtc
     9121 agcactgcca taacttgtca cattgcaaaa gtagccaaat tgcttcaggc accatcagtt
     9181 caccatcacc aaaaaaaaga aacattccag aacttccgca tctcgcccag ccgctccgac
     9241 ttctatactg aatagaaaac gccaacataa gagaaaccta tgccattacc cgattttcat
     9301 gtttctgaac cttttaccct cggtattgaa ctggaaatgc aggtggttaa tccgccgggc
     9361 tatgacttaa gccaggactc ttcaatgctg attgacgcgg ttaaaaataa gatcacggcc
     9421 ggagaggtaa agcacgatat caccgaaagt atgctggagc tggcgacgga tgtttgccgt
     9481 gatatcaacc aggctgccgg gcagttttca gcgatgcaga aagtcgtatt gcaggcagcc
     9541 acagaccatc atctggaaat ttgcggcggt ggcacgcacc cgtttcagaa atggcagcgt
     9601 caggaggtat gcgataacga acgctatcaa cgcacgctgg aaaactttgg ttatctcatt
     9661 cagcaggcga ccgtttttgg tcagcatgtc catgttggct gcgccagtgg cgatgacgcc
     9721 atttatttgc tgcacggctt gtcacgattt gtgccgcact ttatcgccct ttccgccgcg
     9781 tcgccatata tgcagggaac ggatacgcgt tttgcctcct cacgaccgaa tattttttcc
     9841 gcctttcctg ataatggccc gatgccgtgg gtcagtaact ggcaacaatt tgaagcccta
     9901 tttcgctgtt tgagttacac cacgatgatc gacagcatta aagatctgca ctgggatatt
     9961 cgccccagtc ctcattttgg cacggtggag gttcgggtga tggatacccc gttaaccctt
    10021 agccacgcgg taaatatggc gggattaatt caggccaccg cccactggtt actgacggaa
    10081 cgcccgttca aacatcagga gaaagattac ctgctgtata aattcaaccg tttccaggcc
    10141 tgtcgctatg ggctggaagg cgtcattacc gatccgcaca ctggtgatcg tcgaccgcta
    10201 acggaagata ccttgcgatt gctggaaaaa atcgcccctt ccgcacataa aattggcgca
    10261 tcgagcgcaa ttgaagcact gcatcgccag gtcgtcagcg gtctgaatga agcgcagctg
    10321 atgcgcgatt tcgtcgccga tggcggctcg ctgattgggc tggtgaaaaa gcattgtgag
    10381 atctgggccg gtgactaagt cgggaattgc cttacagaag aatatccctg acaatggtgt
    10441 tttaacttac atttaacaat gttatgaaac accctttaga aaccttgacc accgcagcag
    10501 gcattttgct gatggctttc ctctcttgcc tgctgcttcc cgcccccgca ttggggctga
    10561 cgctggcaca aaaactggtg agcacgttcc atctgatgga tcttagtcag ctttacactt
    10621 tattgttttg tctgtggttt ttagtgctgg gcgctattga gtattttgtt ctgcgcttta
    10681 tctggcgacg ctggttctcg ctggcggatt aaacgtcgat tatcgccgta cttcgcggca
    10741 tactttgctt attctccttc gcctaaagga acgtttatgg ataagcaatc actgcacgaa
    10801 acggcgaaac gcctggccct tgagttaccc tttgtcgagc tttgctggcc ttttggcccg
    10861 gagttcgatg tttttaaaat tggcggcaag atttttatgc tgtcgtcgga gctacgcggc
    10921 gtccccttta tcaatctgaa gtccgatcca caaaaatccc tgttaaatca gcaaatatac
    10981 ccaagcatta agccagggta tcacatgaat aaaaagcact ggatttcggt gtatcccggc
    11041 gaggaaatct ccgaagcgtt acttcgcgat ctgatcaacg attcgtggaa tctggtggtt
    11101 gatggtctgg ctaaacgcga tcaaaaaaga gtgcgtccag gctaaagcgg aaatctatag
    11161 cgcatttttc tcgcttacca tttctcgttg aaccttgtaa tctgctggca cgcaaaatta
    11221 ctttcacatg gagtctttat ggatatcatt tctgtcgcct taaagcgtca ttccactaag
    11281 gcatttgatg ccagtaaaaa cttaccccgg aacaggccga gcagatcaaa actctcctgc
    11341 aatacagccc atccagcacc aactcccagc cgtggcattt tattgttgcc agcacggaag
    11401 aaggtaaagc gcgtgttgcc aaatccgctg ccgataatta tgtgttcaac gaacgtaaaa
    11461 tacttgatgc ctcgcacgtc gtggtgttct gtgcaaaaac cgcgatggac gatgcctggc
    11521 tgaagctggt tgttgaccag gaagatgctg atggccgctt tgccacgccg gaagcgaaag
    11581 ccgcgaacga taaaggtcgc aagttcttcg ctgatatgca ccgtaaagat ctgcatgatg
    11641 atgcagagtg gatggcaaaa caggtttatc tcaacgtcgg taacttcctg ctcggcgtgg
    11701 cggcgctggg tctggacgcg gtacccatcg aaggttttga cgccgccatc ctcgatgcag
    11761 aatttggtct gaaagagaaa ggctacacca gtctggtggt tgttccggta ggtcatcaca
    11821 gcgttgaaga ttttaacgct acgctgccga aatcacgtct gccgcaaaac atcaccttaa
    11881 ccgaagtgta attctctctt gccgggcatc tgcccggcta tttcccctca gattctcctg
    11941 atttgcataa ccctgtttca gccgtcatca taggctgctg ttgtataaag gagacgttat
    12001 gcaggattta atatcccagg ttgaagattt agcgggtatt gagatcgatc acatcacctc
    12061 gatggtgatg attttcggta ttatttttct gaccgccgtc gtggtgcata ttattttgca
    12121 ttgggtggta ctgcggacct tcgaaaaacg tgccatcgcc agttcacggc tttggttgca
    12181 aatcattacc cagaataaac tcttccaccg tttagctttt accctgcagg ggattatcgt
    12241 caatattcag gcggtattct ggctgcaaaa aggcaccgaa gcggcagata ttctgactac
    12301 ctgcgcgcag ttgtggatca tgatgtatgc gctgctttca gtcttctcgt tgctggatgt
    12361 tattttgaat ctggcgcaga aattcccggc ggcatctcag ttaccgctga aagggatatt
    12421 tcaggggatt aaactgatcg gcgcaatcct ggtcggcatt ttgatgatct cgctgctgat
    12481 tggtcagtcg ccagcgattc tgatcagcgg tcttggtgca atggctgccg tgctgatgtt
    12541 ggtttttaaa gatccgattc ttggtctggt ggcaggtatt cagctttccg cgaacgatat
    12601 gctgaaactg ggcgactggc tggagatgcc gaaatacggc gcggatggcg cggtgatcga
    12661 tattgggtta accaccgtca aagtgcgtaa ctgggacaat accattacca ctattcccac
    12721 ctggtctctg gtttctgact cctttaaaaa ctggagcggg atgtcagcat ctggcgggcg
    12781 acgtattaag cgcagtatca gtattgatgt caccagtatt cgttttcttg atgaagacga
    12841 aatgcaacgt ctgaataaag cgcacttgtt aaaaccttat ttaaccagcc gccatcagga
    12901 aattaatgag tggaatcgcc agcaaggttc tacggagtcg atattaaacc tgcgccgaat
    12961 gaccaatatt ggaacctttc gtgcctatct gaacgaatat ctgcgtaacc atccgcggat
    13021 tcgtaaagat atgaccttaa tggtacgcca actggctcca ggtgataacg gtttaccgct
    13081 cgagatctac gcgtttacta acaccgtggt gtggctggaa tatgaaagca ttcaggctga
    13141 tatattcgat cacatatttg cgattgtcga agagtttggt ctgcgacttc atcagtcgcc
    13201 aaccggcaat gatattcgct ctctggcggg tgcatttaag cagtaattaa aaaaaccgct
    13261 ctcatcgaat ggatgagagc ggacatcgtt ttgtcggatg tagcgtgaat gccttatttc
    13321 cgacgcagcg ttttaaatgc cacaaacagg aatacaatcc acaccggcag caggatcgct
    13381 gacaagcgca tatcatccat cgtgcacatc agcagcaaaa tcatgccgag gaaggcaatg
    13441 caaagatagt tgccgaacgg atagagcagc gccttaaact gtgtttcacg cccctgacgt
    13501 cgcatcgctg cacgaaaacg cagatgcgcc agacagatca taatccagtt caacagcagc
    13561 gttgctacca ccagcgccat cagcagacca aacgcttttt gcggcagcag atagttgatt
    13621 aataccacca gcgaagtgat cgctccggaa agcatcagcg agttaatcgg cacaccgcga
    13681 cggctgacgc gagtcaaaaa cttcggcgca ttaccctgca cagaaaggcc aaacagcatg
    13741 cggctgttag agtaaacccc gctgttatac actgacagcg atgctaccag aatgacgaag
    13801 ttcagcgcag aagctaccac gttgctgtcg agattatgga aaatcatcac aaacgggcta
    13861 ctgttggatt tcacttccac ccacggatag agcgccagta aaaccaccag tgaaccgatg
    13921 taaaacagca ggatgcgata caccacctga tttaccgctt ttggaatgct tttttccgga
    13981 tcgcgcgctt cagcggcagt aatcccaatc agctccagac cgccgaagga gaacataatt
    14041 accgccagcg acaaaatcag cccattccag ccggtggcaa agaaaccacc gtagcgccag
    14101 aggttgtcga tactggcttt ctcgccgccg tgaccagaaa acagcagcca caggccaaag
    14161 ccgatcatac cgatgatcgc cagcacttta atcagcgcaa accagaactc ggtttcacca
    14221 tataagcgca cgttcaccag gttaacggcg ttgatgataa taaagaaggc ggcagcccaa
    14281 atccacgttg gaacatccgg gaaccagtac tgcatataga tgcccgcagc ggtcagctct
    14341 gccattccca ccagcacgaa cattacccag tagttccagc cagagaggaa gcccgcaaac
    14401 ggtccccagt atttataggc aaagtgggca aatgaaccgg ataccggctc ctcaaccacc
    14461 atttcgccaa gctggcgcat aatcaggaaa gcgatgatcc cggcgacgcc gtagcccagc
    14521 aatacagccg gacccgccat ctgaatcgcc gggccaatgc caagaaacag accagtacca
    14581 attgcgccac ccagcgcaat cagttgaata tgacggttat gtaatccgcg atgaagcgtc
    14641 ggctcttgat tcgacgcagt atcttccgat acggttgacg cgtttttcac gcctttcccc
    14701 tgtgtgtctt ttttgttgag gggcaccttt taacatttag tgcccatcgt cgcaagacac
    14761 aatccacacg gttaaacggg gtatcctgct tttatttccg tacccgatgt cggtgcagcc
    14821 acatcagctt atacgccgcc gggataataa acagcgacag caaaggtgcg gtgatcatgc
    14881 cgccaatcat cggcgcggca atccggctca tcacctctga accagccccc gttccccaca
    14941 gaatcggcag cagaccggcg ataatcaccg ccaccgtcat cgctttcggg cgcacgcgca
    15001 ggaccgcgcc gtgatataac gcctcatcca gcttctgctc gctgaatgtt tgtggattat
    15061 tcaacgacgg cacggcctct atggcgtgac gtaaatacat cagcatcacc acgccaaatt
    15121 cggcggcgac cccggcgagg gcgataaagc cagtgcccgt cgccacggaa agatgaaagc
    15181 ccatccacca cagcaaccag atgccgccca ccagcgcaaa cggtacgctg ctgataatca
    15241 gcaacgcttc gcccacccga cggaacgcca gatacaacag cacgaagata atcatcaacg
    15301 tcatcggcac catgagttta agcttatggt tggcgcgctc cagtagctcg aactgcccgg
    15361 agaatgccac gctggtgccc ggttttaact gcactttttc agctatcgct ttttgcaaat
    15421 cgtgaaccac cgacaccatg tcacgatcgc gggcatcgat ataaatccag ctcgtcgggc
    15481 gcgcattctc ggttttcagc atcgacggtc cggtagagac tttaatgtcg gccacgtctg
    15541 ccagggtgat ttgctgcttc atcggcgtca ggatcggctg ctggcgcagt gcctgcggac
    15601 tatcgcgcca gctttgcgga taacgcagat taattggata acgggcaatc ccttccaccg
    15661 tttcgccaac catcgccccg cccaccgcag aagtcacaaa caactgcaca tccgccaccg
    15721 tcataccgta acgtgcggct ttttcacggt taatctcaac gttgatatag cgcccacctt
    15781 ccagccgctc ggcaagcgca gaagctacgc ctggcaccgt tcgcgctact tcttcaattt
    15841 gctcagccat cgcgtcgata tccgccagca cagtgccgga aactttaatg ccgatggggc
    15901 ttttaatgcc ggttgagagc atatcgatac ggttacgaat tggcggcacc cacagattcg
    15961 ccagccccgg cagccgcacg gtgttatcca gttcctcaat gattttgtcc atcgtcatgc
    16021 ctggccgcca ctgctcctgc ggcttaagct ggatggtcgt ttctaccatc tccagcggag
    16081 cagaatcggt ggcggtttcc gctttcccgg ttttgccaaa tacccgcgcc acttcaggta
    16141 cgctcataat tagcttgtcg gttttttgca gcatactcgc cgcctctgct gcggaaatcc
    16201 ccggcagcgt cgatggcata tacaacaagt cgccttcatt gatctgcggt aaaaattccc
    16261 cgccaacttt attgagcggc cagagaaccg tcagcaccga aagcgccgcc accagcagcg
    16321 tggttttcgg ccagtgcagt actttcagca acagcggatg ataaacacga atcaaaaagc
    16381 gattgagcgg gttactgctt tccggcggaa ttttgccacg gatccagtag cccatcagga
    16441 tcgggatcac tacgatcgcc agcagcgccg cacccgccat cgcatacgtt ttggtgaacg
    16501 ccaacgggcc aaacagacgc ccttcctgcc cttccagggt gaagatcggg ataaacgaca
    16561 acgtgataat cagcagactg ataaatagcg ccggccccac ttcaacagac gcatcggtga
    16621 tcacctgcca gcgcgtttta ttatccagcg tggcgtcagg atgctggtgc tgccactctt
    16681 ccagccgttt atgcgcattc tcgatcatga cgatagcagc atcgaccatc gccccgacgg
    16741 caatcgcaat gccacccaac gacataatat tggcattcag tccctggaag tgcatgacaa
    16801 taaaagcaat acacaacccc agcggcaacg aaataatcgc caccagcgcc gagcgcacat
    16861 gccagagaaa cagcgcacag actaccgcca ccacaataaa ctcttccagc aacttgccgc
    16921 tgaggttgtc gatggcgcgg tcaatgagct ggctgcgatc gtatgttgta actatctcca
    16981 cgccttccgg cagactactt ttcagcgttt ccagtttgtc cttcacggcg gcgatcactt
    17041 ctcgggcgtt tttgccggat cgcaggatca ccaccccgcc cgccacttcg ccttcgccgt
    17101 ttagttcggc aatgccccgg cgcatctccg ggccaatctg gaccttcgca acatcgcgca
    17161 gataaacggg cacgccattt tcactggctt ttaaaacgat gtgattaaag tcgtcgagcg
    17221 tttgcagata gccgctggcg cgcaccatat attccgcttc cgccagttcg atcgacgaac
    17281 cgcccgcttc ctggtttgaa gcatccagcg cgctttttac ttcggcgaga ctgatgccat
    17341 actgcgccag gcgctgggga tcgataacca cctgatactc tttcaccaca ccgcccaccg
    17401 acgccacttc cgcaacgtca gggatggttt tcagctcata tttgagaaac cagtcctgta
    17461 atgagcgtaa atcggccaga tcgtgcttac cgctgcgatc caccagtgca tattcataga
    17521 tccagccaac acccgtggca tctggcccca gctcggcgct gactcccgca ggcagcttac
    17581 cctgtacctg gttgagatac tccagcaccc gcgaacgcgc ccagtacgga tcggtgccat
    17641 cttcgaaaat gacatacaca taagagtcgc caaactgtga gaaaccgcgc acagtcttcg
    17701 cgccaggcac cgacaacatg gtggtggtta gcggataagt cacctgattt tcaacgattt
    17761 gtggtgcctg accgggatag ctggttttaa taataacctg cacatcggag agatccggca
    17821 gcgcatccac tggcgtatta atgatggtcc aggtgcccca gatgctcaga aacaacgcgc
    17881 ccatcagcac caaaaaacgg ttcgccaccg agcgacgaat aatccattca atcattggtt
    17941 attccctcaa tgcgcatggg tagcactttc agagcgcatc cgctccagtg cgccagaaat
    18001 attggcttcg gaatcaatca ggaacaggcc gctggaaacc accttttcac cttccgccag
    18061 accagagcgt aatgcggtga cgccttgcga tgcctggaaa acagcaacgc gtttcggtac
    18121 aaagcgcccg tcggcatcaa cggtaatcac ccgctgttcg ctgccggtat caatcagcgc
    18181 ttgtgacgga atgagcagca tcggttcgct ggcggtgttg agttgcaacc aggcgttcat
    18241 gcccggtttt agcgcctcgt cggcgttgtc gacttccaga cgcagctgca gcgtgcgggt
    18301 cgcggcatcc acgccaggta gcagcgtcca tttgcggatg gtgagtgttt tatccggtcg
    18361 cgccggaacg gtgagggtaa actgcgaggc atctttcacc agccaggcga tggactccgg
    18421 gatcgcagca gtgacccaca ccgggtccat accctgaatt ttcgcgacca cgttatcttt
    18481 ggcgatattc attcccgcgc gcagatcaaa cgcggtgatc acgccatcaa tgggcgcttt
    18541 gagcgtaaag cgagtctgga ttttttgcgt ggcgatcagg cggcgaatat ccgcctccgg
    18601 cattcccgcc agtcgcagcc gctcaagaat gccttcagtc tgggtcgccg taccgccggt
    18661 ttcgcgcagc agtaaatact cactctgcgc ttccacccag tcaggaatgg tcaggtcgag
    18721 aagcggtgtg cccttttgta ctttatcgcc cacggtaagc ggatacacct tgtcgataaa
    18781 cccggcagcg cgagcctgca caatggcata ctgatactcg ttgtaactga cattcgccgg
    18841 gaaactctgg gcaaaagtca gcggtccgcg cgtgacggta gccgttttca cccccagatt
    18901 ctgcgtctga gtcgggtcaa tgcgcacacc agacgcagaa ctctcttcat cggcatattt
    18961 cggcaccaga tccatatcca taaacggcga tttacctggt ttatcgaacc gcgtattggg
    19021 atacattggg tcgtaccaga ataagatttt acgttctgcg gtcgacgttt tttctgcagg
    19081 cggttccgcc tttgcaaccc aggtaaagcc tgccgcagaa ataataccgc ccgcgatcat
    19141 gctgccgata ataagcgcga tttttttcat ctcattaaac ctgggttact ggctgacttt
    19201 aatatcctgt aataaagaaa ggttgccctg ctggacaaaa ttaaacgcca ctttgtcgcc
    19261 ggttttaatt tcactcattt tcgtctgcgg ggtgatggta aagcgcatgg tcatctccgg
    19321 ccagttcaca gcagcaatcg gatcgtgatg gatggtgatt tttttgcttt ccagatcaat
    19381 accctttacc tcgccagtgg cactaataac ctgtggttgt gcttcgctca tggtttcatg
    19441 atgatgttcg ttagcctggg cattaaagcc aataacggta aacagactga acattgcgac
    19501 ttgcagtgct tttttcattt attctctcct ggagttaaaa gttattgctg ccaaccgcca
    19561 ccaagtgcgg tatataaaga aatttcgtta acctgacggg cataattcag atcaagtaaa
    19621 gtttgtcggg ttgcaaataa agaacgctcg gcatccagca cttccagata acttactgcg
    19681 ccgtgctgat ataatgcccg cgcccgttgc aaagtaattt gcagcgatgc cagataacgc
    19741 tgctgggcgc taatttgatc gttcaggctt tgacgtaatg ccagcgcatc tgccacttct
    19801 ttaaaggcgt tctggatttt ctgttcataa ttcaccaccg actgctgctg gcgaatttcg
    19861 gcgatatcca gattggcctg gttgcgtccg gcattaaaaa tggggatctc aattttgggt
    19921 ataaaattcc acatcccgct gctggcgtta aataatgacg atagatcgct actggcggtc
    19981 gatattccgc tggtcaggct tatagacgga aaaaatgccg cgcgtgcagc gccaatattg
    20041 gcattagccg ccattaacgc gtgttcagct tccataatat ccgggcgctg caataagatt
    20101 tgcgacgaca atcccgccgg taatttaacg ctttgcaggc tgtcgctgtt tactgtctgc
    20161 gcttgcggca gcttgccgta gcttcccagc aacagttgca atgcattatt cgcctgcgcc
    20221 agttccccct gacgtttagc gatgtcgctg cgggtacttt ctatcacccc gcgagcctgt
    20281 tccagcgcca gaacattact gctacctgtc aacagttgtt tttcgacaaa cgcatatgac
    20341 tgctgataat tacgcagcgt ttcttcggct atttgcaatt gcgcatacgc cagttgctga
    20401 ttgaaatagc tttgcgcgac attagaaacc agcagaatat gcaccgcgcg ctgagcttcc
    20461 tcagtggcta aataattttg tcgctcggct tcgctcatgt tctttaagcg accgaaaaaa
    20521 tcgaggtcaa agctggcgtt aaggccggtc gaaaactccc gtgtcgtggc ggtattccct
    20581 ttaagattac cgctccagct gccgctgccc tcgccattga gctgtgggta gcggtcggca
    20641 tcggtcagac gatattgcgc ccgtgcctcc tgcactttca gcgtcgccat gcgcaaatcc
    20701 cgattattca ccagcgcctc gctaatcagc gtcttcacct gattatcaac aaaaaaggtg
    20761 cgccagcccg cgttctgata gttatctgct gcgttaacca ggccgttctg gctgagtgag
    20821 aactgctgcg gcacgggcat tgccggacgc tgataatccg gtgccagtga acaaccggtt
    20881 agcgcaaggg ccacacaaaa tggcagaagt ttacaaggag acataggctc ataatttctg
    20941 gtgattttat gccgccaact ttactcgcca ggctctgatt ttccggtgac agaaaaatga
    21001 caaaattgtc attttgccaa taagcgattg ccatctgatc ccgctactct agaattgccc
    21061 gggcaacatg cggaggaaat atgaaactgt tgattgtcga agatgaaaag aaaaccggag
    21121 aatacttgac caaagggtta accgaagccg gttttgtggt cgatttggcc gacaacgggt
    21181 tgaatggcta ccatctggcg atgaccggtg attatgatct gataatcctc gatattatgc
    21241 tgccggacgt gaacggctgg gatatcgtgc gcatgttgcg ttccgccaat aaagggatgc
    21301 cgattctgtt gcttaccgcg cttggcacca ttgaacatcg cgtcaagggg ctggagttgg
    21361 gggcggatga ctatctggtg aagccgttcg cttttgctga actgctggcg cgggtgcgca
    21421 ctctcctgcg gcgcggggcg gcggtgatta tcgaaagtca gtttcaggtt gccgatctga
    21481 tggtcgatct cgtcagccgc aaagtcaccc gcagcggcac gcgcatcact ctgaccagta
    21541 aagagtttac tctgctggag tttttcctcc gccatcaggg cgaagtgctg ccccgctcgc
    21601 ttatcgcctc gcaggtgtgg gacatgaatt tcgacagcga cactaacgcc attgatgtag
    21661 cggtgaagcg gctgcgcggc aaaatcgaca acgactttga gccgaagcta attcagaccg
    21721 tgcgcggcgt gggctacatg cttgaggtgc cggatggtca gtaagccatt tcagcgcccg
    21781 ttttcgctgg caacccgcct gacctttttt atcagcctgg ccaccatcgc ggcgtttttc
    21841 gcctttgcat ggatcatgat ccactcagta aaagtgcatt ttgccgagca ggatattaat
    21901 gatttaaaag agattagcgc cacgcttgaa cgggtactta atcaccctga cgaaacgcaa
    21961 gcccgacgct taatgacgct ggaagatatc gtcagtggtt attccaacgt gttgatttcc
    22021 ctggcagata gccacggcaa aacggtgtat cactcccccg gtgcgcctga tatccgcgag
    22081 tttgcacgtg atgccatacc cgataaagac gcgcggggtg gcgaggtgta tctcctttcc
    22141 ggcccgacga ttatgatgcc aggccacggt cacgggcata tggaacacag caactggcgg
    22201 atgattaact tgtcggttgg cccgttggtg gacggcaaac cgatttatac gctctacatc
    22261 gcgctttcga tcgattttca tcttcattac ataaatgatt tgatgaataa acttattatg
    22321 accgcatcgg taatcagcat cctgatcgtc tttatcgtac tgttggcggt acataaaggt
    22381 cacgcgccga tccgcagcgt cagccgtcaa atccagaata tcacctcgaa agatctcgac
    22441 gttcgccttg acccgcagac cgtgcccatt gagctggaac agctggtact gtcgttcaac
    22501 catatgatcg agcgtattga ggatgtcttt acccgccagt ccaatttctc agcggatatc
    22561 gctcacgaaa ttcgcacgcc gattaccaat ctcatcacgc aaacggaaat cgccctcagc
    22621 cagtctcgca gccagaagga gctggaagat gtgctctact ctaatctcga agagctgacg
    22681 cgaatggcga aaatggtcag cgatatgctg tttctcgctc aggccgataa caaccagcta
    22741 atccccgaaa agaaaatgct caacctggcg gatgaagtcg gcaaagtgtt cgattttttc
    22801 gaggcgttag cggaagatcg cggcgtggag ttgcgatttg ttggcgacga atgtcaggtt
    22861 gcgggcgatc cgctgatgct gcgtcgggcg ttaagcaacc tgctttctaa tgccctgcgt
    22921 tatacgccgc ccggagaggc aattgtagtg cgctgccaga cggtcgatca ccaggtgcaa
    22981 gttaccgtcg aaaaccccgg tacgcccatt gcgcccgagc acttaccgcg attgtttgac
    23041 cgtttctacc gcgttgaccc ttcccgccag cgaaaaggcg aaggtagcgg cattgggctg
    23101 gcgatagtga aatcgattgt tgtcgcgcat aaaggcacgg ttgcagtaac gtcagatgcg
    23161 cgggggacaa ggtttgtgat tatgctgccg gagagagagt gattattaag ttcaaattca
    23221 gggcgtagtg catcacctcc tggtattgtt gatcaac
//
LOCUS       DBANUU010000077        22121 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000077 DBANUU010000000
VERSION     DBANUU010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.077"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..471
                     /locus_tag="ACIE68_002874"
     CDS             <1..471
                     /locus_tag="ACIE68_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich domain-containing protein"
                     /protein_id="HFX5909160.1"
                     /translation="FFGAGGGAGGTVITTLILTKDSYPVTIGAGGAGGVSATNGLNGG
                     DSSFGSVTAPGGEGGGKSGFTNTNGGNGGVPSTGGINIIGGNGGDGQSGNIGVSGEGG
                     TSYWGGGGRAGAGGGVSGKAYGSGGGGAYDAGYSGTSMTGGKGAAGICIIEEFA"
     gene            555..881
                     /locus_tag="ACIE68_002875"
                     /pseudo
     CDS             555..881
                     /locus_tag="ACIE68_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554845.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(853..995)
                     /locus_tag="ACIE68_002876"
                     /pseudo
     CDS             complement(853..995)
                     /locus_tag="ACIE68_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415673.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(1041..1592)
                     /locus_tag="ACIE68_002877"
     CDS             complement(1041..1592)
                     /locus_tag="ACIE68_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HFX5909161.1"
                     /translation="MLHTMNFTVPVNQSTVNALISNCLGAINQGATELNIYISSPGGD
                     LVAGFTAYHFLKALPIPVKTHNLSNVESVANIIFLAGSERKANPGSRFLLHPFHWGLM
                     GPSVDHSRVSEWGACLNNDLERFVDILESQIELSKSRNEWKEVISAATIANASTALDW
                     GMVHEVAAATYPQAPGTYWWINC"
     gene            1702..2115
                     /locus_tag="ACIE68_002878"
                     /pseudo
     CDS             1702..2115
                     /locus_tag="ACIE68_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            2258..3430
                     /locus_tag="ACIE68_002879"
     CDS             2258..3430
                     /locus_tag="ACIE68_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFX5909162.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTAGGKAGESGTLARSLDAIADQAKPVTVVVRVPQGETEEETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDNKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQNDVTTLIRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLTDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRSLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFASQVSA"
     gene            3440..3955
                     /locus_tag="ACIE68_002880"
     CDS             3440..3955
                     /locus_tag="ACIE68_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFX5909163.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRGGGMP
                     GAVDVDLGLDDSALDTEFSIGGTELLLFKQMGKSTVDGIQLRFTGSIQRDDTGEVQAV
                     ELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVDGVD
                     LMEAHRNALGL"
     gene            4010..4312
                     /locus_tag="ACIE68_002881"
     CDS             4010..4312
                     /locus_tag="ACIE68_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFX5909164.1"
                     /translation="MSDKQTEKTIQLDTPIMRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            4327..4446
                     /locus_tag="ACIE68_002882"
     CDS             4327..4446
                     /locus_tag="ACIE68_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFX5909165.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRYKAIQRSGANDE"
     gene            4439..7516
                     /locus_tag="ACIE68_002883"
     CDS             4439..7516
                     /locus_tag="ACIE68_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFX5909166.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRAAQASSKELAGAIRNSRDALK
                     QLNQAGNSLEKFRKLQADNKKLGDRLNYARQKANLLSSELEAMEQPSQRHLVALGRQT
                     LAVQRLEEQQKYLQKQTALVRAELYRAGISAKDDAGATARLARETSRYNQELSKQEAR
                     LKRLGEAQRRMNAARASYARSLEVRDRIAGAGATTTAAGLAMGTPVMAAVKSYTSMED
                     AMKGVAKQVNGLRDDNGNRTARFYEMQDAIKAASEQLPMENGAVDFAALVEGGARMNV
                     ADPDDSWEDQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATMQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLPELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLLV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSVTGALRRWVEANPELTGTLMKVAAIVAAVT
                     VGLGTLAVALAAVLGPLAVIRLGFSVLGIKTLPSVTAAVTRTSSALSWLAGAPLALLR
                     HGLALSGNAAGLLTAPLSSLRRTASLTGNVLKTVAGAPVALLRSGLSGLRAVAVMFMN
                     PLAVLRGGLTAAGTVQRVLASGPLAMLRVALYAISGLLGALLSPIGLVVTALAGVALV
                     VWKYWQPITAFLGGVVEGFKAAAGPVSAAFEPLKPVFQWIGDKVQALWGWFTDLLTPV
                     KSTSAELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKEAAKAKL
                     PESVTRQQPATVNADGKVMMPSGGFPSWGYGFAGMYDSGGYIPRGQFGIVGENGPEIV
                     NGPANVTSRRNTAALAAVVAGMMGVAAAPAELPPLHPLALPAKGGEAIVSRAATVPLV
                     QRIEAPTQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYDA"
     gene            7513..7998
                     /locus_tag="ACIE68_002884"
     CDS             7513..7998
                     /locus_tag="ACIE68_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFX5909167.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PENDMLTLSGVLMPEITGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQDTATSALSDISKTVGGLL
                     S"
     gene            7995..9095
                     /locus_tag="ACIE68_002885"
     CDS             7995..9095
                     /locus_tag="ACIE68_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFX5909168.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGRDITTVLDNRLMSLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQLLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAARHKLKMALGKDLSDKPVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSVTGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPIDDAE
                     WTITTLTHTVSPDNGFTTSIELEVKIDDFEME"
     gene            9185..9403
                     /locus_tag="ACIE68_002886"
     CDS             9185..9403
                     /locus_tag="ACIE68_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFX5909169.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(9639..11324)
                     /gene="ybjL"
                     /locus_tag="ACIE68_002887"
     CDS             complement(9639..11324)
                     /gene="ybjL"
                     /locus_tag="ACIE68_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFX5909170.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            11594..11971
                     /gene="ybjM"
                     /locus_tag="ACIE68_002888"
     CDS             11594..11971
                     /gene="ybjM"
                     /locus_tag="ACIE68_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFX5909171.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(12001..12258)
                     /gene="grxA"
                     /locus_tag="ACIE68_002889"
     CDS             complement(12001..12258)
                     /gene="grxA"
                     /locus_tag="ACIE68_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFX5909172.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            12418..12705
                     /gene="ybjC"
                     /locus_tag="ACIE68_002890"
     CDS             12418..12705
                     /gene="ybjC"
                     /locus_tag="ACIE68_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFX5909173.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            12689..13411
                     /gene="nfsA"
                     /locus_tag="ACIE68_002891"
                     /pseudo
     CDS             12689..13411
                     /gene="nfsA"
                     /locus_tag="ACIE68_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
     gene            13472..14374
                     /gene="rimK"
                     /locus_tag="ACIE68_002892"
     CDS             13472..14374
                     /gene="rimK"
                     /locus_tag="ACIE68_002892"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFX5909174.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            14462..14938
                     /gene="ybjN"
                     /locus_tag="ACIE68_002893"
     CDS             14462..14938
                     /gene="ybjN"
                     /locus_tag="ACIE68_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFX5909175.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            15289..16401
                     /gene="potF"
                     /locus_tag="ACIE68_002894"
     CDS             15289..16401
                     /gene="potF"
                     /locus_tag="ACIE68_002894"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFX5909176.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHVYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            16496..17629
                     /gene="potG"
                     /locus_tag="ACIE68_002895"
     CDS             16496..17629
                     /gene="potG"
                     /locus_tag="ACIE68_002895"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFX5909177.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            17639..18592
                     /gene="potH"
                     /locus_tag="ACIE68_002896"
     CDS             17639..18592
                     /gene="potH"
                     /locus_tag="ACIE68_002896"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFX5909178.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLSEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            18589..19434
                     /gene="potI"
                     /locus_tag="ACIE68_002897"
     CDS             18589..19434
                     /gene="potI"
                     /locus_tag="ACIE68_002897"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFX5909179.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            19494..19982
                     /gene="ybjO"
                     /locus_tag="ACIE68_002898"
     CDS             19494..19982
                     /gene="ybjO"
                     /locus_tag="ACIE68_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFX5909180.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            20023..21150
                     /gene="rlmC"
                     /locus_tag="ACIE68_002899"
     CDS             20023..21150
                     /gene="rlmC"
                     /locus_tag="ACIE68_002899"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFX5909181.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(21325..22056)
                     /gene="artJ"
                     /locus_tag="ACIE68_002900"
     CDS             complement(21325..22056)
                     /gene="artJ"
                     /locus_tag="ACIE68_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFX5909182.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
BASE COUNT         5310 a         5388 c         6156 g         5267 t
ORIGIN      
        1 tttttcggtg ctggcggcgg ggcaggtggg acagtaatca ccacgctgat cctgacgaag
       61 gatagttatc ctgtcactat cggcgcaggt ggggccggcg gcgttagtgc gacgaacggc
      121 ctcaatggcg gtgatagctc gttcggatcg gtaacagccc ctggtggtga aggtggtgga
      181 aaatcaggat tcacaaacac gaacggtggt aacggcggtg tgccaagtac tggcggtatc
      241 aacatcattg gtggaaatgg aggcgacggt cagtccggaa atatcggcgt cagcggtgaa
      301 ggcggaacat cgtactgggg gggcggtgga cgcgcaggcg ctggcggtgg tgttagtggt
      361 aaggcatatg gttcaggtgg cggtggcgca tacgatgccg gttatagcgg aaccagtatg
      421 acaggcggga aaggtgctgc tgggatttgt attatcgagg agtttgcata atgaatgcgt
      481 catatgcagt tattgaaaat gggatggttg tgaatgtcat tgtctgggat ggcgaggctg
      541 aattcacagt gccggataat cagcagctca ttaatatttc tgatatcagt gagcagcccg
      601 gaatcggctg ggtttattca gacggggtat ttactgcgcc gctccctccg gaacgttctc
      661 atgatgaact ggtagctgac gctgaacaga aaaaacagtc gctgatagac gcagcaatgg
      721 ccaatatcag cgtgattcag ttaaagctgc aggccgggcg caaactgacg caagaagaaa
      781 ctacccgact taacgttgtg ctggattata tcgacgctgt gacggcaaca gataccagca
      841 ccgcaccgga tgtcatctgg cctgaactgc cggaggagta ggccattcaa tatctggcgc
      901 actggaggta ttaaccagct ccagtgcatc cagataatcc agccacagat taaactgcac
      961 ttttcatcat cagacaatcg tccaggcgcg agcctggatg ctggcatctc tgcccaattt
     1021 aaatttgaga ggtaattatg ttaacagtta atccaccagt atgtgccggg tgcctgcggg
     1081 taagtggcgg cggcaacctc gtgaaccatc ccccaatcca gagctgtgga ggcattggct
     1141 atggtggcgg cggagataac ctccttccac tcattgcggc tcttactgag ctcaatctga
     1201 ctctccaaaa tatcgacaaa acgctccagg tcattattga gacacgcgcc ccactcactg
     1261 acgcgtgagt gatccacgct tgggcccatc aagccccagt gaaaaggatg cagtaagaac
     1321 cgactcccag gattcgcctt acgttcagac cctgcaagaa aaatgatatt agcgactgat
     1381 tcaacattgc taaggttatg ggtctttact gggattggta gggcctttag aaagtgataa
     1441 gcagtaaaac ctgcaacgag gtcaccgccc ggactggaaa tgtaaatgtt taactcagtc
     1501 gccccctgat tgatagctcc aaggcaattg ctaattaaag cgttaacggt ggactgattt
     1561 acgggcaccg tgaaattcat agtatgcagc atcactatct ccttgtttag ttagtggaca
     1621 cattaccctg ggagcaataa aaaacataac gcatactgca tgcttagttg gtgaccggcg
     1681 atctaagttt agcaaggatg catatctgaa ggcgatacgc tggtggtctg aaagcgtgac
     1741 cgcctcggtc gcagcatgcg acatcttgtt gggttagtgg aggaactgcg ggagaaaggt
     1801 gttaacttcc ggagcctgac ggatagcatc gatacgagta cgcctatggg gcgcttcttc
     1861 tttcatgtga tgggtgccct ggctgaaatg gagcgagagt ttattgtcga acgtactagg
     1921 gcagggctgg ctgcagctcg tgctaaaggc agagtaggtg gacgccgtcc taagttgacc
     1981 accgaacagt gggcacagat tgggcgttta ctcgaggccg gagaatcaag acagcgtatt
     2041 gcactgattt ttgatgtagg cgtttctacc atgtatagaa aatttccggc aaataagagc
     2101 aatgaatccc cctgaatcag cattattttg attatccctg caagtagaca aataccgtca
     2161 ttttgtatga ataacggtac aactgcgctt agctgtttgt caggcacaat cacttcaaca
     2221 tagggcgaag cctaatccaa tcaggaggtt cgccactatg gctcaggatt accaccacgg
     2281 ggtgcgcgtt gttgaagtca acgaaggcac ccgatccatt accacggtga gcaccgccat
     2341 cgtgggtatg gtctgcacgg gcgatgatgc cgatgcaaaa atgtttcctc ttaataaacc
     2401 cgtgctgatc actgatgtgc tgactgccgg cggtaaagcg ggtgagtccg gtactctggc
     2461 ccgttcgctg gatgccatcg ctgaccaggc aaaacccgtg accgttgttg tgcgtgtgcc
     2521 gcagggtgaa acggaagaag aaaccacgac caatatcatc ggcgcagtga ctgctgaagg
     2581 taaaaaaaca ggcatgaaag ctctgttatc tgcccagtca cagctcggcg ttaaaccgcg
     2641 cattctcggc gtgccaggcc acgataacaa agccgttgcg actgagttgc tgagcgtggc
     2701 gcaaagcctg cgtgggtttg cttacctgtc agcgtatggc tgcaagacgg tacaggaggc
     2761 gatcacttac cgtgaaaact tcagccagcg cgaagggatg ctgatctggc ctgactttac
     2821 gggctgggac acggtgctga atgccgaagc aacggcatat gccactgctc gtgcgctcgg
     2881 tctgcgcgcc aaaattgatg agcagaccgg atggcacaaa agcctgtcca acgtgggcgt
     2941 gaacggtgtc accggaattt ctgctgatgt gttctgggat ctgcaggacc cggcaaccga
     3001 tgcaggtctg cttaaccaga acgacgttac cacgcttatc cgcaaagacg gtttccgctt
     3061 ctggggttcc cgctgtctga gcgatgatcc gctctttgcc ttcgaaaact acacccgaac
     3121 cgcgcaggta ctgacggaca cgatggcaga agcgcacatg tgggcggtgg acaaaccgct
     3181 gaatccgtcg ctggctcgcg acattatcga aggtatccgc gccaaaatgc gcagcctggt
     3241 cagtcagggg tatctcattg gtggtgattg ctggctggat gagtcggtga acgacaaaga
     3301 cacgctgaaa gccggaaaac tcaccatcga ctatgactac acgccagtgc cgccacttga
     3361 aaatctgatg ctgcgccagc gcatcaccga tcagtacctg gtgaatttcg ccagccaggt
     3421 cagcgcgtaa ggggacaaca tggctttacc acgcaaatta aaacatctga acttgtttaa
     3481 cgacgggaac aactggcagg ggatcgttga gtcgctgacg ctgccgaaat tcacccgcaa
     3541 atatgagaag tatcgcggcg gcggtatgcc gggggcggtg gatgtggatc tgggactgga
     3601 tgacagtgca ctggatacag aattttccat tggtggtact gaactgctgc tgtttaagca
     3661 gatgggcaaa tccacggtgg atggcatcca gctgcgcttt accggctcta tccagcgtga
     3721 cgatactggg gaagtgcagg ccgtggagct tgtcgtgcgt ggacgtcaca aagaagtgga
     3781 ttccggcgag tggaagacgg gcgaaagcaa caccaccaaa gtgaccagta ccaacagcta
     3841 cgcgaagctg accatcaatg gtgaggtgct ctatgaagtg gaccttatca acatggtgga
     3901 aattgtggac ggtgtggacc tgatggaagc gcaccgcaac gccctcggcc tctgatgtat
     3961 ctgaacggcg cggaatgccg cgccagaacc taatttacag gacaacaaaa tgagcgataa
     4021 acagactgaa aagaccattc aactggatac ccccatcatg cgcggtaaaa cagaaattac
     4081 cgaaattgtg ttgcgtaaac cgcagtctgg tgcgctgcgc ggcacacgcc tgcaggccat
     4141 tatggatatg gatgtgaacg cgatgatgac cgtgatcccc cgcatctcca gtccggcact
     4201 gactgcacag gaaatcgcag agatggaccc ggcagatctc acagccatgt cggttgaggt
     4261 tgtcactttt ttgttgaaga agtcggtgct tgccggttta ccgacagcct gacggttgac
     4321 gatctggtgg cagatatcgc caccattttt cactggccgc catccgttac tgacgttatg
     4381 ccgctgaccg aagtgctgga atggcggtat aaagcgattc agagaagcgg ggccaacgat
     4441 gagtgataac aacctgcgcc tgcaggtcat tcttaatgcg gttgacaaac tcacccgccc
     4501 attccgtgct gcacaggcca gttcgaaaga gctggctggt gcaatcagaa actcccgtga
     4561 cgcattaaag caactcaatc aggcgggtaa cagcctggaa aaatttcgca agctgcaggc
     4621 cgataacaag aagttaggcg acaggctgaa ctatgccaga cagaaggcta atttgcttag
     4681 ctctgagctg gaggcgatgg aacaaccatc acaacggcac cttgtggctt taggtcggca
     4741 aacgctggca gtccaacgcc tggaagaaca acaaaaatat ttgcagaagc aaacggcgct
     4801 tgtgcgtgca gaactgtacc gggcgggaat ttctgcgaaa gatgatgcgg gagcaactgc
     4861 ccgtttagcc cgtgaaacat cacgttataa ccaggaattg tcgaaacagg aggcgcggct
     4921 gaagcgactg ggtgaagctc agcgcaggat gaatgcggcg cgtgccagtt atgcccgttc
     4981 gctggaggtg cgcgatcgta ttgcaggagc cggagccact accacggctg cagggctggc
     5041 aatggggacg ccagtgatgg cggcagtaaa aagctatacc agcatggaag atgccatgaa
     5101 aggtgtggca aagcaggtca atggtctgcg tgacgataat ggaaaccgta ctgcacgttt
     5161 ttatgaaatg caggatgcca tcaaggctgc cagcgaacag ttgccgatgg aaaacggtgc
     5221 ggtggacttc gctgcactgg ttgaaggtgg tgcgcgcatg aacgtcgcag accctgacga
     5281 cagctgggaa gaccagaaac gtgacctgct ggccttcgcc agtacggcag caaaggcggc
     5341 aacagccttt gagctgccag cggatgaact gtcagaaagt ctggggaaaa tcgcccagct
     5401 ctacaaaata cctacccgca atattgaaca gctcggcgat gcgctgaact atctggatga
     5461 taacgccatg tcgaaagggg cggacatcat tgatgtgatg caacgtctgg gcggtgtggc
     5521 tgaccgtctg gattatcgta aagcggcggc actgggttcc acctttctga cactgggcgc
     5581 tgcgccggag gttgcagcca gtgcagcaaa cgcgatggtg cgtgaattgt ccattgccac
     5641 catgcaaagc aagagtttct ttgaagggat gaatctgctg aaactcaatc ctgaagtgat
     5701 tgaaaagcag atgacgaagg atgcgatggg aactatccag cgtgtgctgg agaaggtgaa
     5761 cgcactgccg caggacaagc gtctgtctgc catgaccatg ttgtttggta aagagtttgg
     5821 cgatgacgcg gcgaaactgg caaacaacct gccggaacta cagcgccagc taaaactgac
     5881 agcgggcaat gatgcgctcg gttccatgca gaaagaatcc gacatcaaca aggactcact
     5941 ttctgctcag tggttgctgg ttaaaaccgg agcgcagaat accttcagca gcctgggcga
     6001 aacgctgcgc cagccgctga tggatattct gtacacggtg aaaagcgtta cgggagcgtt
     6061 gcgtcgctgg gtggaagcta acccggaact gacgggcaca ctgatgaaag tagccgctat
     6121 tgtggctgcg gttaccgtcg gcctcggcac cttggctgtg gcgctggctg cagtgctggg
     6181 gccgctggca gtcatccgtc tgggattctc tgtgctgggc atcaaaacgt taccttccgt
     6241 tacggcagca gtaacacgaa ccagcagcgc gttgtcctgg ctggctggcg caccactggc
     6301 actgctgcga cacgggcttg ctttatcggg taacgcagcg ggtttactta ctgcgccgtt
     6361 gtcgtctttg cgccgtacgg catcactgac gggaaatgtc ctgaaaactg ttgcaggtgc
     6421 gccggttgca cttttgcggt ctggattatc cggtttacgt gcggttgctg tgatgtttat
     6481 gaatcctctg gcggtactgc gcggtggact gaccgccgca ggtacggtgc agcgagtact
     6541 ggcatctggt ccactggcga tgctgcgcgt tgccctgtat gccatatctg gtctgttagg
     6601 tgctctgctc agtccgatag gtctagtggt tactgcactg gcaggcgtgg cgctggttgt
     6661 ctggaaatac tggcaaccca tcaccgcatt tctcggtggc gtggtggagg gattcaaagc
     6721 ggcggcaggt cccgtcagtg ccgcattcga accacttaag cctgtgttcc agtggattgg
     6781 cgacaaagtg caggcgctgt ggggctggtt tactgacctg ctgacgcccg ttaagtcgac
     6841 ctctgccgaa ctgcagagtg cagcggcaat ggggcgacga ttcggggagg ctctggcgga
     6901 agggctgaat atggtcatgc atccgctgga ctccctgaaa tccggcgttt cctggttgct
     6961 ggagaaactc ggcattgtca gtaaagaggc cgcaaaggca aaactaccgg aaagcgtgac
     7021 gcgtcagcaa cctgcgacgg tgaatgcaga cggcaaagtg atgatgccat cgggtggttt
     7081 tccatcatgg ggatatggct ttgcggggat gtatgacagc ggcgggtata tcccgcgcgg
     7141 gcagtttggc atcgtcggtg aaaacgggcc ggaaattgtt aacggcccgg caaatgtgac
     7201 cagccggaga aatacagctg cactggctgc cgttgttgcc ggaatgatgg gcgttgctgc
     7261 cgcgccagca gagcttccac cgttgcaccc tttggcactt cccgcgaaag gtggagaagc
     7321 aattgtgagt cgcgcagcca ctgtgccgct cgttcaacgg attgaggcac cgacgcagat
     7381 catcattcag acgcagccag gacaaagtgc gcaggatatt gcgcgggagg tggcacgcca
     7441 gcttgatgaa cgtgaacgca ggctgaaggc aaaagccagg agtaactaca gcgatcaggg
     7501 gggatacgac gcatgatgat ggtgctggga ttgtacgtgt ttatgctgcg caccgttccg
     7561 tatcaggaac tgcagtatca acgcagctgg cgacatgcgg caaacagtcg ggtaaaccga
     7621 cgtccgtcca cgcagtttct gggaccggaa aacgacatgc tgacgctttc cggtgttctt
     7681 atgccggaga taacaggcgg caggctgtcg ttgctggcac tggagcagat ggcagaacag
     7741 gggaaagcat ggcccctgat tgaaggcagc ggcacgattt acggcatgta tgtgattgag
     7801 ggactgaatc agactaaaac ggagtttttc cgcgacggta tgccgcgccg gattgagttc
     7861 accctgtcgc tcaaacgagt ggatgaatcc ctgtccgata tgttcggtga tctcagtgcg
     7921 caactgaata atctgcagga cacggcaacg tctgccttaa gcgatatcag taaaacggtg
     7981 ggagggctgc tgtcgtgaat ttcagctcag aactgcttaa caaaggcaac aaaactcccg
     8041 cattcagcat cagtattgag ggcagggata tcaccacagt gctggataac cgcctgatga
     8101 gtttgacgct gacggataac cggggctttg aagcggacca gcttgatctg gagttggatg
     8161 acgctgacgg aaaaatcgtg ctgccgcgcc gtggtgcggt cattacgctg gcgctgggct
     8221 ggaaggggca gctgcttttc ccgaaagggg cattcacagt ggacgagatt gaacacactg
     8281 gcgcaccgga ccgcctgact atccgggcgc gaagtgctga ttttcgggaa acgctgaata
     8341 cccgccgtga aaaatcgtgg cataagacca ccgtcgggga agtggtgaag gaaatagccg
     8401 cgcggcacaa gctgaagatg gcactgggta aagacctgtc ggataagccc gtggagcata
     8461 tagaccagac taatgagagt gacggcagtt ttctgatgcg gctggcgcga caatacggtg
     8521 ccatcgcgtc ggtgaaaaat ggcaatctgt tattcatccg gcaggggcag ggcaaaagcg
     8581 tcactggtaa acctctgcca gtgatcacta tcacacgcaa ggacggcgac agtcaccgat
     8641 ttaccctggc agatcgcgga gcctacacgg gcgtaattgc cagctggttg catacccgcg
     8701 aacctgcgaa gaaagaaagc accacggtga agcgtaagcg cagaactaag aagcagaaga
     8761 aagagccaga agcgaagcag ggcgattacc tggtgggtac ggatgaaaac gtgctggtac
     8821 ttaatcgcac ttatgccaac cggagcaacg ccgaacgagc ggcgaaaatg cagtgggaac
     8881 gcctgcaacg cggcgttgcg tcattttcgc tacaactggc agaagggcgg gcagatctct
     8941 acacggaaat gcctgtgaag gtcagtggtt ttaaacagcc gatagatgat gcggaatgga
     9001 ccattacgac tctgacacat accgtcagcc cggataacgg ttttacaacc agtattgaac
     9061 tcgaagtgaa gattgatgat ttcgaaatgg aatgattctt cgcaatggag aacttttaag
     9121 tttgcaaaat ggaataatgc ggtatcatta ttgtgaattt agcaaaaatg ggagaactcg
     9181 aaaaatgatg atttgcccac tgtgtggaag tgccgcccat actcgcagca gttttcaggt
     9241 atcttcattg accaaagagc gttacaacca gtgccagaat attaactgca gccatacttt
     9301 tgtaacccat gaaacttttg ttcgttcgat tgcaacgcca aaagagtcaa atccggttca
     9361 gccgcatcca atgaaatcag gacaggtggc gctctctctt tgacgctgcc gccattttgt
     9421 cgccatcgtt aaaaaacagt gcttctaacg tcatgatttt aaacgacata aatttcaggc
     9481 aacaaaaaac ccatcaacct tgaaccgaaa tggcggggtt gatgggctcc acaaaatggg
     9541 gacatcaaag aaaagcagtg gcactaatta agactgatgc cctgcggaaa agttctgcgg
     9601 ttgtgcaaaa aaatttcatt ttcagggcaa cttcagtttt atcctaatcc tggccatacc
     9661 atgacgatga ttgtccctgc cagcgtcagc aggacgttgg cgattgcgta ggtgcccgca
     9721 tagcccagcg cagggatgtt actgcgagct gtatcactga tgatctccat tgccggcgcg
     9781 caggtgcgtg cgcccatcat tgcgccgaac aacagtgcgc ggttcattcg caatacataa
     9841 gcaccgaaca agaaacagat aaccacgggc acaagactga caattaatcc ggcaatcaac
     9901 atctgaccgc caatcgcgcc caggccgtta ttaataccgc taccggcgct cagaccaacg
     9961 cctgccataa acaccatcaa gccgaactct ttcaccatgc ttaatgcacc ttgcggaatg
    10021 taaccgaagg tcgggtggtt agcacgcata aagcccagca taattccggc gaataacaac
    10081 ccggcagcgt tccccatgcc gaaactgaat gtgctgaact ggaaggtgat catcccgatc
    10141 atcagcccaa taacaaagaa ggcgcagaat gccagcaggt cagtgacctg gctgtgaatc
    10201 gagataaagc cgatgcgatc ggcgatggtt tttacgcggc gggcatcgcc gctgacttgt
    10261 aaaacgtcac ctttgttaag cacgacgttg tcatctatcg gcatctcaat ctggctacga
    10321 atgacgcggt taaggaagca accgtgatcg gtcaacttca gttgtgcgag acgtttacct
    10381 acagcgttat ggtttttaac gaccacttct tcagtgacga tacgcatgtc gagaaggtca
    10441 cgatcgaaaa cttctttacc gttacggaag ctgggatcga gtcgggcatg ggcgtcggga
    10501 tagcctacca acgctatttc atcgcccatt tgtagcacgg catcaccgtc tggatttgcc
    10561 agaatcccgt tacgtcgaat acgttcaatg tagcagccgg tttgtcgata aatacccagt
    10621 tcacgcagat ttttgccgtc ggtccaggcc accagttccg ggccgacgcg ataggcgcgg
    10681 atcaccggta aataaacctt acggttggca tcagtgtcca ggccacgttc gcgggcgatt
    10741 tgctgggcgc tggtctgtaa gtcctgatgc tgcaatttcg gcaagtaacg cgcaccaaca
    10801 atcaaactca ccagaccgat taaataggtt aaggcatacc cgaggctcag attatccagt
    10861 gccagtgaga gctgcctgct ttccatgccg gaatgacgca gtgtatcgcc agcaccgacc
    10921 agaaccggtg tcgacgtcat agagcctgct aacataccgg ccgtcaggcc aatatcccag
    10981 ccaaacagct tacctaaccc taaggcgatc accagcgcac tgccaaccat caccagtgct
    11041 aacattaggt aatttttccc atcgcgaaaa aaaatggaaa aaaagttcgg tccggcttcg
    11101 accccgacgc agaaaataaa cagcataaag ccaagattaa gcgcatcggt gttaatgctg
    11161 aaatgttgtt ggcctaataa cagcgatacg actaaaacgc caatggaatt acccagttgg
    11221 atcgaaccaa gtcgtaactt tccgagacat agcccaagcg cgaggaccac aaataataac
    11281 agaatgtaat tcccatttaa caattcggcg acgtttatat tcacggaggc taacttcttg
    11341 tttactagta agctgttgaa agaaatggta atttacgata atgtttttta ccagaattca
    11401 gggcgcagat tcattcagcg cacctaaacg atagtaaagt aacaatatat tttactagtg
    11461 taatcacatt aggtatcaac ggctatatga attgcgttgg cctatattag catggaatgc
    11521 gaagcggctt tatcttactg aacgccacac tggcgaaaaa tgtgttcgat agacgcagtg
    11581 tcaggaggaa cgagtgaaac ataaacaacg ttgggcgggg gcaatctgct gttttgtcct
    11641 cttcattgtg gtgtgccttt ttctggcgac gcacatgaaa ggcgcttttc gggctgccgg
    11701 gcatcctgaa atcggcttgc tatttttcat tcttcctgga gcagtcgcca gcttcttttc
    11761 acagcgtaga gaagtcctga aacctctgtt tggcgcaatg ctggcggcac cctgttcgat
    11821 gctcattatg cggctgtttt tttcaccgac gcgctcattc tggcaagagc tggcatggtt
    11881 actaagcgcg gtgttctggt gtgcgctggg ggcactgtgt ttcttattta tcagtagttt
    11941 gtttaaacca cagcacagaa aaaatcagta aagccctcaa cgcgagggct tgtcagacga
    12001 tcaggcgtcc agattttctt tcacccatgc agcaaaatcg gtatagccgc cgatatgttg
    12061 ctgatcgaca aaaatctgcg gcacggtttc tacgggttta cctgcctttt gttgtagatc
    12121 ttctttagtg atcccttccg cacgaatatc tacatactga tactgaaaat catcgcgttc
    12181 attgctcaat ttctcagcca gatcttttgc acgcacacag taagggcaac ccgaacgacc
    12241 aaaaataacg gtttgcatta tttctctcct catagattta tgcctgtaat gatcacgcta
    12301 aaatgtattc gctgaaagta ggtttaacct gttgcattaa ttgctaaaag ctataactgt
    12361 taaacacaat acagtgaaaa gttttagact gaaggctcac tttgcagagg gaagcgtatg
    12421 cgcgcgatcg gtaaattgcc taaaggcgtg ttgatactgg aatttatcgg aatgatgcta
    12481 ctggcggtgg cgctgctgtc ggtaagcgac tccctgtcgc tgcctgagcc attttctcgg
    12541 ccagaagtgc agattctgat gatttttctc ggtgttttgc tcatgcttcc cgctgcggtg
    12601 gtggttattc ttcaggtggc aaaacgtctt gccccacagc tgatgaaccg tccaccgcaa
    12661 tattcacgtt cagaaagaga aaaagataat gacgccaacc attgaactta tttgtggcca
    12721 tcgctccatt cgccatttca ctgatgaacc catttccgaa gcgcagcgtg aggcgattat
    12781 taacagcgcc cgtgcgacgt ccagttccag ttttttgcag tgcagtagca ttattcgcat
    12841 taccgacaaa gcgttacgtg aagaactggt gacgctgacc ggcgggcaaa aacacgtagc
    12901 gcaagcggcg gagttctggg tgttctgtgc cgactttaac cgccatttat agatctgtcc
    12961 ggatgctcag ctcggcctgg cggaacaact gttgctcggt gtcgttgata cggcaatgat
    13021 ggcgcagaat gcattaatcg cagcggaatc gctgggattg ggcggggtat atatcggcgg
    13081 cctgcgcaat aatattgaag cggtgacgaa actgcttaaa ttaccgcagc atgttctgcc
    13141 gctgtttggg ctgtgccttg gctggcctgc ggataatccg gatcttaagc cgcgtttacc
    13201 ggcctccatt ttggtgcatg aaaacagcta tcaaccgctg gataaaggcg cactggcgca
    13261 gtatgacgag caactggcgg aatattacct cacccgtggc agcaataatc gccgggatac
    13321 ctggagcgat catatccgcc gaacaatcat taaagaaagc cgcccattta ttctggatta
    13381 tttgcacaaa cagggttggg cgacgcgcta aaaccgccac gtcgatgtat gatacgcggg
    13441 cttttgacca ggtctgacag agaggtgcag ggtgaaaatt gccatattgt cccgggatgg
    13501 aacgctctat tcgtgtaagc ggctgcgtga agccgctata cagcgcggtc acctggttga
    13561 aattcttgat ccgctttctt gctacatgaa cataaatcct gcggcgtctt ctattcacta
    13621 caaaggccgc aagttacccc attttgacgc agtgatcccg cgtattggca ccgccattac
    13681 cttttatggg acggcggcac tgcgccagtt cgagatgctg gggagctatc cgctcaatga
    13741 gtcggtcgcc attgcccggg cgcgtgacaa attgcgttcc atgcaactgc tggcgcgtca
    13801 gggcatcgac ctgcctgtca cgggcattgc gcattcgccg gatgatacca gcgatttaat
    13861 cgacatggtc ggtggtgcgc cgctggtggt caagttggtt gaaggcacgc agggaattgg
    13921 cgtcgtgctg gcggagacgc gtcaggcggc ggaaagcgtg atagacgctt tccgcggtct
    13981 gaacgcgcat attctggtgc aggaatatat caaagaggcg caagggtgcg atatccgctg
    14041 tctggttgtt ggcgatgaag tggtcgctgc gattgaacgg cgggcgaaag agggcgattt
    14101 tcgttccaat ttgcatcgtg gcggcgcggc aagcgtcgcc agtatcacac cacaggagcg
    14161 tgaaatcgcg ataaaagccg cgcgaacgat ggcgctggac gttgctggtg tggatattct
    14221 gcgtgctaat cgcgggccgt tggtgatgga ggtgaatgcg tcgccggggc tggaaggaat
    14281 agaaaaaacc accggtatcg acatcgcggg taaaatgatc cgctggatcg aacgccacgc
    14341 tacgaccgaa tattgcctga aaacgggtgg ttagtcgcaa tcacattact gatcatggtt
    14401 ttgcctgcgc tttttgcgta agctgtgccg gtctttttat cgaaagaggt tgtacaaaat
    14461 tatgacatcg ctggtcgttc ctggtctgga tacgctgcgt caatggctcg atgacctggg
    14521 gatgagtttt tttgaatgtg ataactgtca ggctctgcat ctgccccata tgcagaattt
    14581 cgacggtgtc tttgatgcca aaatcgatct gatcgataac acgatcctgt tttctgccat
    14641 ggcggaagtc cgaccttcag ccgtattgcc gctggcggcg gatttatctg ccatcaatgc
    14701 cagttcgctg accgtgaaag catttcttga tatgcaggat gataatctgc caaagctggt
    14761 ggtttgccag tctttatccg ttatgcaggg cgtaacctat gagcagtttg catggttcgt
    14821 gcgtcagagc gaagagcaga tttcgatggt cattcttgaa gctaatgccc atcaactgct
    14881 gttaccgact gacgatgaag ggcaaaacaa cgttaccgaa aactatttcc tccactgata
    14941 actcctttcg agcacgcagt cgctggtgca gtggctgcgc gctgcaaaat tatctgctgt
    15001 ttttaacctt ttcttaaaga ttatttcact tctcttgtgt cgatttggct ttatcacata
    15061 gagcaaatac gcataaaaat ttgttaaata ccgtttttta atccgagcta tagtctcaaa
    15121 ccctggctaa agttattctt gcgatgcttt tatatagcga gcagtgctgg ccgggagaga
    15181 gttctctttt cttacacccc gccgataaaa aatatgcacg tttattgcat atcttttagt
    15241 gtgacaactt ttgttcgttt gttaacgaac tttcagaagg aaagagatat gaccgcctta
    15301 aataaaaaat ggctttcggg tctggctgcg ggtgctctga tggccgtctc tgtcggcacg
    15361 ctcgcggctg aacaaaaaac actccacgtt tataactggt ctgattatat cgccccggac
    15421 acggtggcca attttgaaaa agaaaccggt attaaagtcg tctacgatgt tttcgactct
    15481 aacgaagtac tggaaggcaa attaatggcg ggtagcaccg gctttgactt ggtggttcca
    15541 tctgccagct ttcttgaacg ccagttgact gcgggagttt tccagccgct ggacaaaagc
    15601 aaattgccgg agtggaagaa tctcgacccg gagctgctga agctggtcgc caaacacgat
    15661 cccgacaata aatttgctat gccctatatg tgggcgacga ccgggattgg ctataacgtt
    15721 gataaagtta aagctgtgct gggcgaaaac gcgcccgtcg atagctggga cttgatcctc
    15781 aaacctgaaa atctggaaaa actgaaaagc tgcggtgtct ctttcctgga tgcgccagaa
    15841 gaagtttttg ctaccgtgtt gaattatctc ggcaaagatc ccaacagcac taaagcagat
    15901 gattacaccg gaccggctac cgatctgctg ttaaagctgc gcccgaacat tcgttatttc
    15961 cattcatcgc aatatattaa tgacctggcg aatggtgaca tctgcgtagc gattggttgg
    16021 gcaggcgatg tctggcaggc gtcaaaccgc gcgaaggaag cgaagaatgg cgtgaatgtc
    16081 tcgttctcga ttccgaaaga gggggcgatg gcattctttg atgtattcgc catgcctgcc
    16141 gatgccaaaa ataaagacga agcctatcag ttcctgaatt acctgctgcg cccggatgtg
    16201 gtggcgcata tttccgacca tgtgttctat gccaacgcca ataaagcagc cacgccgctg
    16261 gtgagtgcgg aagtccgtga gaacccgggt atttatccgc ctgcggatgt tcgtgcgaag
    16321 ctgttcactc tgaaagtgca ggacccgaaa atcgaccgtg tgcgcacccg cgcgtggacc
    16381 aaagtgaaga gcggaaaata atccgcagcc ttagatgccg ggcgggcgca ccacacccgc
    16441 cggcaattcg caccatcatg gtgcgcttgc acacattcaa tgccggagag cagccgtgaa
    16501 tgacgctatc cctcgcccgc aggcgaaaac ccgtaaggcg ctgacgccgc tattagaaat
    16561 ccgcaacctc accaaatcct acgatggtca acatgcggtg gatgatgtca gcctgaccat
    16621 ctacaaaggt gaaatcttcg cgctgctggg cgcatccggc tgtggcaagt ccacgctgct
    16681 gcgtatgctg gcaggtttcg aacaaccttc tgccggacag ataatgcttg atggcgtcga
    16741 tttgtcacag gtgccgcctt acctgcgccc catcaatatg atgtttcagt cttacgcgct
    16801 gtttccgcat atgaccgtgg aacagaacat cgcttttggc ctgaaacagg acaaactacc
    16861 gaaagcggaa attgccagtc gggtccatga gatgctcggc ctggtgcaca tgcaggagtt
    16921 cgccaaacgc aaaccgcatc agctttccgg tggtcagcga cagcgtgtgg ccctggcccg
    16981 aagccttgcc aaacgcccga aactattact gctcgatgag ccgatgggcg cgctggataa
    17041 aaagctgcgt gaccggatgc agcttgaagt ggtggatatt ctggagcgcg tcggtgtgac
    17101 ttgtgtgatg gtcacccacg atcaggaaga ggcgatgacc atggcggggc gtatcgccat
    17161 tatgaatcgc gggaaatttg tccagattgg cgagccggaa gagatctacg agcatccaac
    17221 tacccgctac agcgctgaat ttatcggttc ggttaatgtc tttgaaggtg tactcaaaga
    17281 gcgtcaggaa gatggcctgg tgcttgattc gccggggctg gtgcatccac tgaaagtcga
    17341 tgcggatgct tcagtggtcg ataacgtgcc ggtacacgta gcgctgcgcc cggaaaaaat
    17401 catgctttgc gaagagccgc ccgccaatgg ttgtaacttc gcggtggggg aggtgataca
    17461 cattgcctat ctcggcgatc tttcggtgta tcacgttcgt ctgaaaagtg ggcagatgat
    17521 tagcgcccag ctacaaaacg cccatcgtca tcgtaaaggg ttaccgacct ggggcgacga
    17581 agtgcgtttg tgctgggaag tggacagctg tgtggtgctg acggtttaag gagcaaagat
    17641 gaatacactt gaacctgctg cccagtcgaa accgccgggc ggatttaagc tgtggttgtc
    17701 gcagctgcaa atgaagcatg ggcgcaaact ggtcattgcg ttgccatata tctggttgat
    17761 cttgctgttt ctgctgccat ttctgattgt ctttaaaata agcctgtcgg agatggcgcg
    17821 cgctattcca ccttataccg aactgatgga gtgggctgac gggcaactgt ccatcactct
    17881 taatctcggt aattttctgc aactgaccga cgatccgctc tatttcgatg cttatctcca
    17941 gtcgctacag gtggctgcga tttcgacatt ttgctgttta ctgatcggct atccgctggc
    18001 gtgggcggtg gcgcacagta agccttcgac ccgtaatatt ttattactac tggtgatcct
    18061 gccgtcgtgg acctcgtttc tgatccgcgt ttatgcctgg atgggaatat taaaaaacaa
    18121 cggtgtgctg aataattttc tgctgtggct gggggttatc gatcaaccgc tgaccattct
    18181 tcataccaat ctggcagttt atattggcat tgtttacgcc tacgtgccgt ttatggtact
    18241 gccgatttat accgcgttga ttcgtattga ttattcgctg gtggaagcag cgctggatct
    18301 cggtgcacga ccgctgaaaa cgttctttac tgtgatcgtg ccgctgacta aaggtgggat
    18361 tattgccgga tcgatgctgg tgtttatccc ggctgtgggc gagtttgtga tcccggaact
    18421 gctcggtggc ccggacagca tcatgatcgg gcgcgtgcta tggcaagagt ttttcaataa
    18481 ccgcgactgg ccggtggcct cggcggtagc gatcatcatg ttgctgctgc taattgtgcc
    18541 gataatgtgg ttccacaaac accagcaaaa aagcgtggga gaacacggat gaataattta
    18601 ccggtagttc gttcaccctg gcggattgtg attttgctgc tgggctttac ctttctctac
    18661 gcgccaatgc tgatgctggt tatctattcg tttaacagct cgaagctggt gacggtgtgg
    18721 gccggctggt caacgcgctg gtatggtgag ttattgcgcg atgacgcgat gatgagtgcg
    18781 gttggtttaa gcctgacaat tgctgcctgt gcggcaacgg cggcggcgat cctcgggact
    18841 attgcggcgg tggtgctggt gcgctttggc aggtttcgcg gatcaaatgg ctttgccttt
    18901 atgatcaccg cgccgctggt aatgccagat gtcatcacgg gcttgtcgct gttgttatta
    18961 ttcgtcgcgc ttgctcatgc cattggctgg cctgcggacc gcggtatgct caccatctgg
    19021 ctggcgcatg tcactttttg tacggcttat gtggcggtcg ttatttcttc aagattgcgg
    19081 gaactggatc gctcgataga agaagccgcg atggatctcg gtgcgacgcc gctgaaagtg
    19141 tttttcgtca ttacgctacc gatgatcatg cccgcgatca tttctggctg gttactggct
    19201 tttactttgt cgcttgatga tctggtgatc gccagctttg tttccgggcc gggagccacc
    19261 acgttaccga tgctggtctt ttccagcgtg cggatggggg tgaatccgga aatcaacgcc
    19321 cttgcaacgt taattctcgg tgcggtcgga attgtcggat ttatcgcctg gtatctgatg
    19381 gctcgcgcag aaaaacagcg gatacgcgat atccagcgtg caagacatgg ctgaagacac
    19441 taaaatttgc caacctggct acataatgcc gcgcatgccg cggcattgtt ttcatggaag
    19501 acgaaacgtt gggatttttt aagaaaacat cttcatctca tgctcgcctg aatgtgcctg
    19561 cgctggtgca ggtagcggcg ctcgccatta ttatgatccg tggcctcgac gtgctgatga
    19621 ttttcaatac gctgggcgtg cgcggtattg gcgagttcat tcatcgcagc gtacaaacct
    19681 ggagtttaac gctggtcttt ttaagcagtc tggtgctggt tttcattgag atctggtgtg
    19741 cgttttcact ggtgaaaggg cgtcgctggg cgcgctggct atatctgctg acacaaatca
    19801 ccgccgcaag ttacttgtgg gcggcttcgc tggggtacgg ttatccggag ctgttcagca
    19861 ttcccggtga atcaaaacgt gaaatcttcc atagcctgat gctgcagaag ctgccggata
    19921 tgctcatcct gatgctgctg ttcgttccct cgaccagtcg gcggttcttc cagttgcaat
    19981 aatgtgtata atcgtcgccc ctgatgatgt gaaggtcaat gtatgcagtg cgcactttac
    20041 gacgcgggtc gctgtcgttc ctgtcagtgg ataacgcagc cgattccaga gcaactctcc
    20101 gctaaaaccg ccgatcttaa aaatctgctc gccgattttc cggttgagga atggtgcgcg
    20161 ccggtgtcag gcccggaaca agggtttcgt aataaagcca aaatggtggt gagtggtagc
    20221 gttgaaaaac cactgctcgg tatgctgcat cgcgacggca caccagaaga cctttgtgac
    20281 tgcccgcttt atcctgcctc atttgcgccc gtttttgcgg cgctaaaacc gtttatcgcc
    20341 cgtgcggggc tgacacccta caacgtggcg cgtaagcgtg gcgaactgaa atacattctg
    20401 ctgactgaaa gccagagcga tggcggcatg atgctgcgct ttgtactgcg ttctgaaacc
    20461 aaactggcac aactgcgtaa ggcgctgccg tggttacagg aacaactacc gcaactgaaa
    20521 gttattaccg tcaatattca gccggtacat atggcgatta tggaagggga gacggagatc
    20581 taccttactg aacaacaggc gctggcagag cgttttaatg acgtaccgct gtggatccgt
    20641 ccgcaaagtt tcttccagac taatccggcg gtcgccagcc agctgtacgc taccgcgcgc
    20701 gactgggtac ggcagctgcc agttaaacat atgtgggatc tgttctgcgg tgtgggtggc
    20761 tttggtttac actgcgcgac gcctgacatg cagttaaccg ggatcgaaat tgcatcagag
    20821 gccattgcct gtgcaaagca gtcagccgct gaactgggct taacgcgttt gcaatttcag
    20881 gcgctggact ccactcagtt tgccaccgct cagggggagg tgccggagct ggtgctggtt
    20941 aatccgccgc gccgcggcat tggtaaaccg ctatgtgatt atctctcaac aatggcaccg
    21001 cgttttatta tctactccag ctgtaacgcc caaaccatgg cgaaagatat ccgcgaactg
    21061 ccaggttacc gcatcgaacg ggtacagctt tttgatatgt tcccgcacac cgcgcactat
    21121 gaagtgctga cgctgctggt gaagcaataa aaaagccgca ggtgcggctt cagattgctg
    21181 atcaggtgcg ctttgttcat gccggatgcg gcgtgtacgc cttatccggc ctacaaaatc
    21241 atgctaaatc aatacattgc agagttgatg tgggccggat aagcgcagcg catcaggcaa
    21301 tcttactttt gtcatcagtc tcaattactg cggaaaccac tggtcattga ttttctgata
    21361 tgtaccgtca gctttaattg ctgccagcgc gttattcagt ttttccagca gagctttgtt
    21421 atccggacgt acagcgatgc ccagaccggt gccgaagtat tgcggatcgg tcactttttc
    21481 agtagcaacg cccagttgcg gattggtttt cagccattcg tttaccaccg ctgtgtcacc
    21541 aaatacccca tcaatacgac catttttcag atcgataaag gcattctgat aactgtcata
    21601 ggagacagtt ttcacttcgg ggtgctgatc ctgaatatat ttctggtgcg tggtgccgtt
    21661 ttccatccca atacgtttgc ctttcagatc ggcaaacgtt ttgtaggtat cttttttggc
    21721 aatcacgacg gctgagtttt catagtaggg cgtggtaaac gatacctgct tgctacgctc
    21781 gggcgtgata tccataccgg agattacggc gtcatatttt ctgaatttca gtgacgggat
    21841 caggctgtcg aacgcgtgat tagtaaaagt acattctgcc tgcatttgtt tgcacaaggc
    21901 ttttgccaga tcgatatcaa agccgacaat ctcattatta gcacctatag attcaaaggg
    21961 tggataggtg gctgaaacgc caaaattgat tttctctgcg gcagaagcac cgaaagtaaa
    22021 ggaagcaagt aaagcggcaa gaactaactt tttcatgatg gaactcccgt ctgtcaatct
    22081 tatgattttt ggccgtgtct gcggcatggg ataacaatgc c
//
LOCUS       DBANUU010000078        22106 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000078 DBANUU010000000
VERSION     DBANUU010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.078"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1007)
                     /gene="malZ"
                     /locus_tag="ACIE68_002901"
     CDS             complement(<1..1007)
                     /gene="malZ"
                     /locus_tag="ACIE68_002901"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFX5909183.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RL"
     gene            complement(1163..2536)
                     /gene="proY"
                     /locus_tag="ACIE68_002902"
     CDS             complement(1163..2536)
                     /gene="proY"
                     /locus_tag="ACIE68_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFX5909184.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(2612..3931)
                     /gene="brnQ"
                     /locus_tag="ACIE68_002903"
     CDS             complement(2612..3931)
                     /gene="brnQ"
                     /locus_tag="ACIE68_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFX5909185.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(4338..5633)
                     /gene="phoR"
                     /locus_tag="ACIE68_002904"
     CDS             complement(4338..5633)
                     /gene="phoR"
                     /locus_tag="ACIE68_002904"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFX5909186.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLGGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(5691..6380)
                     /gene="phoB"
                     /locus_tag="ACIE68_002905"
     CDS             complement(5691..6380)
                     /gene="phoB"
                     /locus_tag="ACIE68_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFX5909187.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            6570..7772
                     /gene="sbcD"
                     /locus_tag="ACIE68_002906"
     CDS             6570..7772
                     /gene="sbcD"
                     /locus_tag="ACIE68_002906"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFX5909188.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            7769..10912
                     /gene="sbcC"
                     /locus_tag="ACIE68_002907"
     CDS             7769..10912
                     /gene="sbcC"
                     /locus_tag="ACIE68_002907"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFX5909189.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRQDELQQEASRRQQALQQVLAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNTLAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQI
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            11038..12222
                     /gene="araJ"
                     /locus_tag="ACIE68_002908"
     CDS             11038..12222
                     /gene="araJ"
                     /locus_tag="ACIE68_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFX5909190.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYMMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(12365..13273)
                     /gene="mak"
                     /locus_tag="ACIE68_002909"
     CDS             complement(12365..13273)
                     /gene="mak"
                     /locus_tag="ACIE68_002909"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFX5909191.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            13398..14309
                     /gene="rdgC"
                     /locus_tag="ACIE68_002910"
     CDS             13398..14309
                     /gene="rdgC"
                     /locus_tag="ACIE68_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFX5909192.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(14387..14470)
                     /locus_tag="ACIE68_002911"
     CDS             complement(14387..14470)
                     /locus_tag="ACIE68_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909193.1"
                     /translation="MTQRPWSKLQRKKHNIAALKIIARRSE"
     gene            complement(14467..14802)
                     /locus_tag="ACIE68_002912"
                     /pseudo
     CDS             complement(14467..14802)
                     /locus_tag="ACIE68_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(14957..15241)
                     /gene="ppnP"
                     /locus_tag="ACIE68_002913"
     CDS             complement(14957..15241)
                     /gene="ppnP"
                     /locus_tag="ACIE68_002913"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFX5909194.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(15313..15990)
                     /gene="aroM"
                     /locus_tag="ACIE68_002914"
     CDS             complement(15313..15990)
                     /gene="aroM"
                     /locus_tag="ACIE68_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFX5909195.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNRGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(16248..16439)
                     /gene="yaiA"
                     /locus_tag="ACIE68_002915"
     CDS             complement(16248..16439)
                     /gene="yaiA"
                     /locus_tag="ACIE68_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFX5909196.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(16489..17013)
                     /gene="aroL"
                     /locus_tag="ACIE68_002916"
     CDS             complement(16489..17013)
                     /gene="aroL"
                     /locus_tag="ACIE68_002916"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFX5909197.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(17190..17654)
                     /gene="yaiI"
                     /locus_tag="ACIE68_002917"
     CDS             complement(17190..17654)
                     /gene="yaiI"
                     /locus_tag="ACIE68_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFX5909198.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAAINPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRGQM"
     gene            17774..18583
                     /gene="proC"
                     /locus_tag="ACIE68_002918"
     CDS             17774..18583
                     /gene="proC"
                     /locus_tag="ACIE68_002918"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFX5909199.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(18600..19715)
                     /gene="adrA"
                     /locus_tag="ACIE68_002919"
                     /gene_synonym="dgcC"
     CDS             complement(18600..19715)
                     /gene="adrA"
                     /locus_tag="ACIE68_002919"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFX5909200.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(19817..20137)
                     /gene="psiF"
                     /locus_tag="ACIE68_002920"
     CDS             complement(19817..20137)
                     /gene="psiF"
                     /locus_tag="ACIE68_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFX5909201.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(20256..21671)
                     /gene="phoA"
                     /locus_tag="ACIE68_002921"
     CDS             complement(20256..21671)
                     /gene="phoA"
                     /locus_tag="ACIE68_002921"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFX5909202.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWQGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(21772..22032)
                     /gene="iraP"
                     /locus_tag="ACIE68_002922"
     CDS             complement(21772..22032)
                     /gene="iraP"
                     /locus_tag="ACIE68_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFX5909203.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         5531 a         5934 c         5534 g         5107 t
ORIGIN      
        1 agccgccagc cgtccatact ccacggcgct ttcagccaat ggcggacaat actgtcttcc
       61 ccgcgataaa tttcattcac cagactttcc gactgataat ccagcttcgg caagctggca
      121 tagccaagcc agtcgagcgc cgtgccatca tcactaaacg agtaccagtc gcgccagggc
      181 gattcagggt tgtgacaagc tccccccgtg ccacgattgt gcctgtcaaa ccaggcatgg
      241 gaatcgccac tgtggttaaa cacgccatcc agcaccagcc gcattcccag ctgctgcgta
      301 ttgtggcgta agcgcagcaa cgccccatca ccgccaaact gcggatcgac atggcgataa
      361 tcctcggtat cgtatttatg tacgctggga gctttaaaca ccggattgag atacagcgcc
      421 gtcacgccaa gctttttcag atacggcagt ttttcgctta tcccgtccag atcgccgcca
      481 tagaacgttg atccgcccgc ctgcgccgtg accggttcat cccagtcacg caagatgatc
      541 tcttgtccgg ctgcatgatg gtaatagaca tgatcctgtt cagcttcacg aggaagacta
      601 cgcgcaaaac gatcagggaa gatctgataa aaaatctgat ccgcagccca ttgtgggccg
      661 atatccggta catcgacggc aaactgctcc agtcgtgccg gcggcattcg gctgaagccc
      721 tgcggtgtaa accaacgctg gcgatcgtgc cacagcagtt tgaaactgta acgccgccgg
      781 ggttgtccgc tggagagatc aatcgccgca cgccatgcgg tgacgccagg ctgctgctga
      841 ctgcgctgct tatgcatcga tactgacatt tcttcgttat cgtgttctgt acgcagcata
      901 atgcgctgcg gtgggtcttc gcccgtcagc cacagtgtaa tgagcagttg atctttgctt
      961 tgtttaacaa atgggggcac cggcaggtgc catgcattta acatcataat tcccctgtga
     1021 tgaaaatctg atcaccttgc catagcctaa tgcaggatca ctcatcatct gaaggatatt
     1081 tgggggagga ggacgggggc ggataacttt cggaatagcc ggatcaggat ttcctcctga
     1141 accggcttac agaacgcaag tattattggt tttcagccag ctgacgatcg tggcggcgtt
     1201 taaacatcca gccaatcaac agcacaacaa tccacgcgaa accgacatac agcgagatac
     1261 gcgtatccgg gtgataacca atcaacccga taataaagag caggaaaatc agcccgccga
     1321 tggtcgttgc taccccaccc ggcactttaa atttcagcgc cttaacttct tctggcggca
     1381 aacggcgacg gaaggcaatt tgcgacagca ggatcataat ccacacccac accgtggcga
     1441 aggttgccag cgaagcgatc accaggaaga cgttttccgg catgatgtag ttcagataca
     1501 ccgcaaacag cagcgcggta gtcatcacca gcaccgtaac ccacggaata ccgcgacgcg
     1561 acgttttgct gaaaattttc ggcgcgctgc cctgctctgc cataccgtgg agcatacggc
     1621 ctacgccaaa tacatcactg ttaattgccg acagcgaagc agtcagcaca acaaagttaa
     1681 gaatgctggc ggcaaaggta atgcccatat gctggaacgt cagcacgaac gggctaccgg
     1741 cagtgccaac ctgattccac gggtagatag acataatgac gaacagcgta ccgacgtaga
     1801 acaccagaat acgcatcggc acggagttaa tcgcacgcgg tatcgatttc tcaggatctt
     1861 tcgcttcacc ggcggtaatc ccgataattt cgatcccacc gtaagcaaac atcaccattt
     1921 gcaacgacat taccatgcca agccagccgt tactgaagaa gccgccgttg ctccacaggt
     1981 tatgaatacc ggtcggttgc ccgccgttgc caatccccca gatgatgatg ccgaaaccgg
     2041 cgacaatcat gatgatgatg gtggcgactt taaagaacga gaaccagaat tccagctcac
     2101 cgaatacctt cacgctcatc aggtttacgg cgcaaatgat cagcaccacg ctcagtaccc
     2161 aaatccagtg cggcaccgtc gggaaccaga cacccatata gataccaaaa gcggtcacat
     2221 cggcgatggc gacaataagg atttcaaagc agtaggtcca gccggtaatg taacctgcca
     2281 gcgggccgag gttttcctgc gcataacgcg agaaagagct ggcggccggg ttatgtaccg
     2341 acatttcccc cagcgcacgc ataatgatat acgccgcgat accaccgata atataggcca
     2401 acaacacgct cggaccggcc attttgatgg cgtctgccga accgtaaaac agcccggtgc
     2461 caattgctga acccagtgcc ataaagcgta tgtggcgggt acttagccca cgctttagct
     2521 tgttcttact ttccatcaat tcctaaccca tcaacacaat aaaaaaccac ggggcatcgc
     2581 agccccgtgg ttaaaacaaa tgttcagtga tttagtgagc gctggaggtc acctgacgac
     2641 ctgccgcgcg atcccagata atggccagaa ccaccatcac cactgttggc attaaccacg
     2701 ccagaccttg ttcggccagc ggtaaacgct gcgcccagga cggtaagata tcgctgaatg
     2761 cagatgcttt gatcccgtcg agaataccaa aaagcaggct gataaacatc ggcggagcaa
     2821 tcacgcggga cgaattatgc caccatgagc gtgtaaaact taatacaacc agtgcgatac
     2881 acggcggata aatagcggtc agcaccggta cggagatctg gatcagctgg cttaagccga
     2941 ggttagaaac caccatcgag aagccgccga ggataaacac cagcgtacga taagagagcg
     3001 gtacgtactg ggcaaagaat tctgcacaag cacaggtcag gccaactgcc gttaccaggc
     3061 aggcgatgaa gattaacgcc gccagcagga agctaccgcc gccgccaaag gtgtgctgaa
     3121 cgtaagcatg cagaatagca gcgccgtttg cagactgatc gaccagcgac gcgctgtctg
     3181 aacccagacg gaacagcgcc aggtacagca gagtcagacc aacacccgcc atcaggccag
     3241 cccagacggt ataacgggtc agtaggcgcg cttcggtaac gccacgagaa cgcgccgcgt
     3301 taacaataac gataccaaac accattgcgc ccagcgtatc catggtcaga tagccgttga
     3361 cgaagccgtt agaaaacgca gcgttttgat aagcctcagt cgccgtgctg atagaacccg
     3421 ccggccagac aatcgcggca acagacagga tgaccagcgc gataattttc agcggcgcaa
     3481 ggaagttgcc cacggtatcc agcagcttgc ccggatagag cgaaaccaga ataacgatag
     3541 cgaaatagac caggctgtag ataaacagcg gcagcgcgga atcacccgtc agcggcgcaa
     3601 tccccacttc aaaagaaacg gtagctgtac gcggcgtagc gaaaagcggc cccaccgcca
     3661 ggtaacaaac cgttgccagc agtacgccag cgactttacc gattggcgtg ctgaggctgt
     3721 caacaccgcc gccaactttt gccagcgcca ctaccgttaa taccggcagg ccaacggcag
     3781 taatgaggaa gccgaatgcc gcagtccaga cgtgttcgcc tgcctgtaag ccgaccattg
     3841 gagggaaaat aatgttacct gcgccgacga acaacgcaaa tgtcataaag cccagagcga
     3901 tgatatcgcg cgatcttaat tgatgggtca taaatcatac tgcctgtgga tgtggtgttg
     3961 taacgttaaa tttttgtgcc aatccctggg gatgatacag cggtatattt aaacaatttt
     4021 agcgggaaat actcccaacc aggcgcggcg aagaatagtt tttcgattta ccgcgttaat
     4081 gcagtcagta tgacagcacc tgaagcgcaa tttaaacgct tataacgttt taaagcaagg
     4141 agggatctaa aaaccagagg tatatagcga taagaaatca ccaaccagta taaaacacta
     4201 tttccgtgaa aaagacatgg ctaatcatgc gaacaaaaaa tcaccaaccg ttgtaaaatc
     4261 taacgcccgt attttgactg caaagaaaat aagccagcat cggctggctt atggcaataa
     4321 aagatgacaa aggcggatta atcgctgttt ttggcaatta aacgttccgg gataacaaaa
     4381 ctgaaacgtg ttccttttcc tactgtactc tcaatattca ggcgactttc gtgatgattc
     4441 accgcatgtt tcacgatcgc taaccctaat ccgctaccgc cggtttgccg ggaacgcgct
     4501 ttatcaacgc gataaaaacg ctcggtcagg cgcggaatat gctccggtgc aatgcccggt
     4561 ccgttatctt caacgctaaa ttcggcaccg tgcggcactc gctgccagcg tacggtgata
     4621 tgcgtgcctt ccggcgtatg attcacggcg ttatagacca ggttcgaaat cgcactgcgt
     4681 agctgatctt cgttgccaga caccttgagg ccgttatcta tctcaaaggt aaatgtctgt
     4741 tttttctgac tcagagtctg agcctcgcgc tcaacaacgc gcagcatcat cggcacatca
     4801 accttttcat tgagcaattg cgtcggtgcg gcttcaattt tcgacagcgt tagcaactgc
     4861 ttcaccagcc cttccatccg ctgggtctgc tcgcgcatgg tgtgcaacgc tttttcgcgt
     4921 accgcgcctc ccagcggctg ctcatccatc atctccaggt aaccctgtaa cacggtcaat
     4981 ggcgtacgta actcatggct cacgttggca aaaaagttac gccgcgcccc ttccagttga
     5041 tgcatttgcg tgacatcacg cgccaccatc agcaactgtt tgtgggtata aggcatgacg
     5101 cgaatttcca gatgccgccc ggtgttgagc accagattga gcgggcgaga aaaatcacgc
     5161 gttttcaaat attgcgtaaa ctccgggtaa cgcagtaggt taaggatgtt ctgcccgtta
     5221 tcttccggcc agcgcaaacc aagaatttgt tgcgccagac cgttacacca gaaaataccg
     5281 ccctcttccg tggtcagcac caccgcgtcg ggcagcgact ccgcgccgct acgaaagcgt
     5341 ttaatcagat tgcccagttc acggcggcgt tttttatttc gcagctgcat ctggtgtaag
     5401 ccgtatagta gcggttccca gctaccacgc cccggtggcg gggtcatact gcgatccacc
     5461 cacagccacc atgaaaggcg caataaattc cagaaatgcc agataagcag tcctgttacc
     5521 gatgccagca aaaaccaggg caggtaacca aaaaatgcac ccaggatgaa agccgggagg
     5581 cagcaaagta gcagctccag caccagcctt ttccacgaca gccgttccag cacgtaagat
     5641 actccagtta agaaatcata agccctgctc tgcgtccgat gagcaaggcg ttaaaagcgg
     5701 gttgaaaaac gatatcctgt accgcgcacg gtctgcacca tgcggtcatg cccgccgggc
     5761 tccagtgctt tacgcaggcg acgaatgtgg acatcgaccg tgcggtcttc cacatacacg
     5821 ttagttcccc agacgtggtt taacagctgc tcgcggctgt acacgcgctc aggatgcgtc
     5881 ataaaaaagt gcagcagttt aaattctgtc ggccccatct ccagcggctc ttcgcccgcc
     5941 atcactcggt gagatgtcgg gtcgagactt aatccctgca tctcaatcac ctcttccacc
     6001 gccattggcg aaatacggcg cattaccgct ttgattcgcg ccaccagctc cttcggcgaa
     6061 aacggcttgg tgatatagtc atccgcgccg gtttcaaggc cgcgcacgcg atcttcttct
     6121 tcccctctgg cggtcaacat caccactgga atatcccggg tcatcgactc gcgcttgagg
     6181 tgtttgatga actggatacc ggagccgcca ggtaacatcc agtcaaggag aattaaatcc
     6241 ggccagggtt cattcagttg attcacagca ctgtcataat cttccgcttc gaccggctga
     6301 aagccatttt gttcgagcac gaagcagacc atttcgcgaa ttggagcttc atcttctacg
     6361 accagaatac gtctcgccat gatttgccct gttgtaataa ataggttgcg atcattaatg
     6421 cgacgtcatt atgcgtcaga tttatgacag atttatgaaa agctcgtcgc acatatcttc
     6481 aggttattga tttccgtggc gcagaaaaaa gcaaatggca catctgtttg ggtataatcg
     6541 cgcccatgct ttttcgccag ggaaccgtta tgcgcatcct tcacacctca gactggcatc
     6601 tcggccagaa cttctacagt aaaagccgcg aagctgaaca tcaggctttt cttgactggc
     6661 tgctggagac agcacaaacc catcaggtgg atgcgattat tgttgctggt gatgttttcg
     6721 ataccggctc gccgcccagt tacgcccgca cgttatacaa ccgttttgtt gtcaatttac
     6781 agcaaactgg ctgtcatctg gtggtactgg caggaaacca tgactcggtc gccacgctga
     6841 atgaatcgcg cgatatcatg gcgttcctca atactaccgt ggtcgccagc gccggacatg
     6901 cgccgcaaat cttgcctcgt cgcgacggga cgccaggcgc agtgctgtgc cccattccgt
     6961 ttttacgtcc acgtgacatt attaccagcc aggcggggct taacggtatt gaaaaacagc
     7021 agcatttact ggcagcgatt accgattatt accaacaaca ctatgccgat gcctgcaaac
     7081 tgcgcggcga tcagcctctg cccatcatcg ccacgggaca tttaacgacc gtgggggcca
     7141 gtaaaagtga cgccgtgcgt gacatttata ttggcacgct ggacgcgttt ccggcacaaa
     7201 actttccacc agccgactac atcgcgctcg ggcatattca ccgcgcacag attattggcg
     7261 gcatggaaca tgttcgctat tgcggctccc ccattccact gagttttgat gaatgcggta
     7321 agagtaaata tgtccatctg gtgacatttt caaacggcaa attagagagc gtggaaaacc
     7381 tgaacgtacc ggtaacgcaa cccatggcag tgctgaaagg cgatctggcg tcgattaccg
     7441 cacagctgga acagtggcgc gatgtatcac aggagccgcc tgtctggctg gatatcgaaa
     7501 tcactactga tgagtatctg catgatattc agcgcaaaat ccaggcatta accgaatcat
     7561 tgcctgttga agtattgctg gtacgccgga gtcgtgaaca gcgcgagcgt gtgttagcca
     7621 gccaacagcg tgaaaccctc agcgaactca gcgtcgaaga ggtgttcaat cgccgtctgg
     7681 cactggaaga actggatgaa tcgcagcagc aacgtctgca gcatcttttc accacgacgt
     7741 tgcataccct cgccggagaa cacgaagcat gaaaattctc agcctgcgcc tgaaaaacct
     7801 gaactcatta aaaggcgaat ggaagattga tttcacccgc gagccgttcg ccagcaacgg
     7861 gctgtttgcc attaccggcc caaccggtgc ggggaaaacc accctgctgg acgccatttg
     7921 tctggcgctg tatcatgaaa ctccgcgtct ctctaacgtt tcacaatcgc aaaatgatct
     7981 catgacccgc gataccgctg aatgtctggc ggaggtggag tttgaagtga aaggtgaagc
     8041 gtaccgtgca ttctggagcc agaatcgggc gcgtaaccaa cccgacggta atttgcaggt
     8101 gccacgcgta gagctggcgc gctgcgccga cggcaaaatt ctcgccgaca aagtgaaaga
     8161 taagctggaa ctgacagcga cgttaaccgg gctggattac gggcgcttca cccgttcgat
     8221 gctgctttcg caggggcaat ttgctgcctt cctgaatgcc aaacccaaag aacgcgcgga
     8281 attgctcgag gagttaaccg gcactgaaat ctacgggcaa atctcggcga tggtttttga
     8341 gcagcacaaa tcggcccgca cagagctgga gaagctgcaa gcgcaggcca gcggcgtcgc
     8401 gttgctcacg ccggaacaag tgcaatcgct gacagcgagt ttgcaggtac ttactgacga
     8461 agaaaaacag ttacttaccg cgcagcagca agaacaacaa tcgctaaact ggttaacgcg
     8521 tcaggacgaa ttgcagcaag aagccagccg ccgtcagcag gccttgcaac aggtgttagc
     8581 cgaagaagaa aaagcgcaac ctcaactggc ggcgcttagt ctggcacaac cggcacgaaa
     8641 tcttcgtcca cactgggaac gcatcgcaga acacagcgcg gcgctggcgc atattcgcca
     8701 gcagattgaa gaagtaaata ctcgcttaca gagcacaatg gcgcttcgcg cgagcattcg
     8761 ccaccacgcg gcgaagcagt cagcagaatt acagcagcag caacaaagcc tgaatacctg
     8821 gttacaggaa cacgaccgct tccgtcagtg gaacaacgaa ctggcgggtt ggcgtgcgca
     8881 gttctcccaa caaaccagcg atcgcgagca tctgcggcaa tggcagcaac agttaaccca
     8941 tgctgagcaa aaacttaata cgcttgcggc gatcacgttg acgttaaccg ccgatgaagt
     9001 tgctagcgcc ctggcgcaac atgccgagca acgcccactg cgtcagcgcc tggtcgcgct
     9061 gcatgggcag attgttcccc aacaaaaacg tctggcgcag ttacaggtcg ctatccagaa
     9121 tgtcacgcaa gaacagacgc aacgtaacgc cgcacttaac gaaatgcgcc agcgttataa
     9181 agaaaagacg cagcaacttg ccgatgtgaa aaccatttgc gagcaggaag cgcgcatcaa
     9241 aacgctggaa gctcaacgtg cacagttaca ggcgggtcag ccttgtccac tttgtggttc
     9301 caccagccac ccggcggtcg aggcgtatca ggcgctggag cctggtgtta atcagtctcg
     9361 attactggcg ctggaaaacg aagttaaaaa gctcggcgaa gaaggtgcgg cgctgcgcgg
     9421 gcaactggat gcattaacga agcagcttca gcgcgatgaa aacgaagcgc aaagcctccg
     9481 acaagatgag caagcactta ctcaacaatg gcaagccgtc acggccagcc tcaatatcac
     9541 cttgcagcca caggacgata ttcaaccgtg gctggatgca caagatgagc acgaacgcca
     9601 gctgcggtta ctcagccaac gacatgaatt acaagggcag attgccgcgc ataatcagca
     9661 aattatccag tatcaacagc aaattgaaca acgccagcag cagctcttaa cggcattggc
     9721 gggttatgca ctgacattgc cacaggaaga tgaagaagag agctggttgg cgacacgtca
     9781 gcaagaagcg cagagctggc agcaacgcca gaacgaatta accgcgctgc aaaaccgtat
     9841 tcagcagctg acgccgattc tggaaacgtt gccgcaaagt gatgatctcc cgcacagcga
     9901 agaaactgtg gcgctggata actggcggca ggttcatgaa caatgtctcg cattacacag
     9961 ccagcagcag acgttacagc aacaggacgt tctggcggcg caaagtctgc aaaaagccca
    10021 ggcgcagttt gacaccgcgc tacaggccag cgtctttgac gatcagcagg cgttccttgc
    10081 ggcgctaatg gatgaacaaa cactaacgca gttggaacag ctcaagcaga atctggaaaa
    10141 ccagcgccgt caggcgcaaa cgctggtcac tcagacagca gaaacgctgg cacagcatca
    10201 acaacaccga cctgacgggt tggctctcac tgtgacggtg gagcagattc agcaagagtt
    10261 agcgcaaact caccaaaagt tgcgtgaaaa caccacgagt caaggcgaga ttcgccagca
    10321 gctgaagcag gatgcagata accgtcagca acaacaaacc ttaatgcagc aaattgctca
    10381 aatgacgcag caggttgagg actggggata tctgaattcg ctaataggtt ccaaagaggg
    10441 cgataaattc cgcaagtttg cccaggggct gacgctggat aatttagtcc atctcgctaa
    10501 tcagcaaatt acccggctgc acgggcgcta tctgttacag cgcaaagcca gcgaggcgct
    10561 ggaagtcgag gttgttgata cctggcaggc agatgcggta cgcgataccc gtaccctttc
    10621 cggcggcgaa agtttcctcg ttagtctggc gctggcgctg gcgctttcgg atctggtcag
    10681 ccataaaaca cgtattgact cgctgttcct tgatgaaggt tttggcacgc tggatagcga
    10741 aacgctggat accgcccttg atgcgctgga tgccctgaac gccagtggca aaaccatcgg
    10801 tgtgattagc cacgtagaag cgatgaaaga gcgtattccg gtgcagatca aagtgaaaaa
    10861 gatcaacggc ctgggctaca gcaaactgga aagtacgttt gcagtgaaat aactattcag
    10921 caggataatg aatacagagg ggcgaattat ctcttggcct tgctggtcgt tatcctgcaa
    10981 gctatcactt tattggctac ggtgattggt agccgttctg gtagttgtga tggtggtatg
    11041 aaaaaagtca ttttatcttt ggctctgggc acgtttggtt tggggatggc cgaatttggc
    11101 attatgggcg tgctcacgga gctggcgcat aacgtaggaa tttcgattcc tgctgccggg
    11161 catatgatct cgtattatgc gctgggagtg gtggtcggtg cgccaatcat cgcactcttt
    11221 tccagccgct actcactcaa acatatcttg ttgtttctgg tggcgttgtg cgtcattggc
    11281 aacgccatgt tcacgctctc ttcgtcttac atgatgcttg ccattggtcg cctggtttcc
    11341 ggctttccgc atggcgcatt ttttggcgtc ggcgcgatcg tgttatcaaa aattatcaaa
    11401 cccggaaaag tcaccgccgc cgtggcgggg atggtttccg ggatgacagt cgccaatttg
    11461 ctgggcattc cgctgggaac gtatttaagt caggaattta gctggcgtta caccttttta
    11521 ttgatcgctg tttttaatat tgcggtgatg gcatcggtct atttttgggt gccggatatt
    11581 cgcgacgagg cgaaaggaaa gctgcgcgaa caatttcact ttttacgcag cccggccccg
    11641 tggttaattt tcgccgccac gatgtttggc aacgcaggtg tgtttgcctg gttcagctac
    11701 gtaaagccat acatgatgtt tatttccggt ttttcggaaa cggcgatgac ctttattatg
    11761 atgttagttg ggctagggat ggtgttggga aatatgctaa gtggcaggat ttcaggacgt
    11821 tattcaccac tgcgcattgc agcagtgact gactttataa ttgtactggc actgctgatg
    11881 ctctttttct gcggcggcat gaaaacaacg tcgcttattt ttgcttttat ttgttgcgcg
    11941 gggttatttg ccctttcagc acctctgcaa atattattac tacaaaatgc caaaggcgga
    12001 gagttattag gtgccgcagg tgggcaaata gcgtttaacc ttggtagcgc cgtcggcgca
    12061 tattgcggtg gtatgatgct gacgctgggg ctggcatata attacgtggc gctgcctgcc
    12121 gccctgcttt cgtttgctgc gatgtcgtca ttgctgctgt atggtcgcta taagcgccag
    12181 caagcggcgg atagtccggt gctggcgaaa ccactggggt agattgcagt cacgttgcgc
    12241 gttgcttatt gccggatgcg gcgtgaacgc cttatccgcc ctacgcggtt ccggcacatt
    12301 ttgtaggcct gataagacgc ggcaagcgtc gcatcaagca tgatgcgcca attgcctacg
    12361 ttttttactc ttgtggccat aaccacgcag cgccgcgtac gccgctggaa tcaccgtgct
    12421 tcgccttacg caccggcgtt tcacattcgc cgccgaagac aaattgttta atcaactgcc
    12481 caaccgtttg atataaacgg tctacattgc tcatcccgcc ccccaggaca atcacatccg
    12541 gatcgagaat attcacgaca tgtgccagcg attttgccag ccgcagctcg tagcgacgca
    12601 atgccagttc cgctaccgga tcgctttctt caaccaggcg gataatttca ctgcctttca
    12661 gcgcatgtcc gctcaaacga cgataatccg tcgcgaatcc cgtgcccgaa ataaaggttt
    12721 caatacaacc ttgtttaccg caataacaag ggacttcctc gcgataacgc agttcgtctt
    12781 cgtccatcca cggtagcgga ttgtgtcccc actcacctgc cgtgccattg ccgccgatat
    12841 gcgcccgccc attgaatgcc acgcccgcgc cgcatcccgt gccgataatc acggcaaata
    12901 ctgtctgcgc tcccgctgcc gcgccatcta ctgcttctga aaccgccaga cagttggcgt
    12961 catttgccag ccgcacttcc cgctgcaacc tcgcgcttaa gtctttatcg aatggctgac
    13021 cgttgagcca ggttgaattg gcattcttaa ccacaccggt gtaaggcgaa attgagccag
    13081 gaatgcccat acctaccgtt ccgcgctgcc ctgtcgcctg ctccgccata tcaaccaacg
    13141 tggcgatcgt ttcaatagtc tgccggtaat catcacgcgg cgtgggcaga cgatggcggt
    13201 acaactgctc ccctgcatcg cccagtgcaa tcacttcagt tttggtgccg cctaaatcga
    13261 tacctatacg cacggtactc tccttatttt tttcaatatc aatagcgtag agacggacaa
    13321 ccggattggc aatgcaaggc cgccgacaat tcgttatcat gcccgctaaa tttaacgaca
    13381 aggccgtgga aattatcatg ctgtggttca aaaatttaat ggtttaccgt cttagccgcg
    13441 agatttcgct gcgtgcagaa gagatggaaa aacagctagc ctcgatggca tttaccccat
    13501 gcggcagcca ggacatggcg aagatgggct gggttcctcc gatgggatcg cacagcgatg
    13561 cgttaacgca cgttgccaat ggtcaaattg ttatctgcgc gcgcaaagaa gaaaaaatcc
    13621 tcccgtcccc ggtgattaaa caggcgctgg aagcgaaaat cgccaaactg gaagcggaac
    13681 aggcgcgtaa gctgaagaaa accgaaaaag attcgctgaa agacgaagtg ctgcactctc
    13741 tgttgccgcg tgctttcagc cgttttagcc agacaatgat gtggatcgac acggttaatg
    13801 gtttgattat ggtggactgc gccagtgcca aaaaagcgga agatacgctg gcattactgc
    13861 gtaaaagcct ggggtcgtta ccggttgtac cattgagcat ggaaaacccg attgaactga
    13921 cgctgaccga atgggttcgc tccggtagtg cggcacaggg cttccagctg cttgatgaag
    13981 ccgagctgaa atcgttgctg gaagatggcg gcgtgatccg cgcgaagaaa caagatctga
    14041 ccagcgaaga gatcaccaat cacattgaag ccggaaaagt ggtgactaaa ctggcgctcg
    14101 actggcagca gcgcattcag tttgtgatgt gcgacgatgg ttcgctcaag cgtctgaagt
    14161 tctgcgacga gctgcgcgat caaaacgaag atatcgaccg tgaagatttc gcccagcgtt
    14221 ttgatgccga tttcatcctg atgactggtg aactggcagc gttaattcaa aacctgattg
    14281 aaggattagg tggcgaagca caacgttaat tgctgatttt cctttaatgc cggatgcgac
    14341 gcctgccgcg tcttatccgg cgtacgaagc cacaccaggc atataattat tcgctacggc
    14401 gagcaataat ttttagcgca gcaatattat gctttttacg ctgtaacttg ctccatggac
    14461 gttgtgtcat tgtttttcct caagccggtg acgcgcttca ctgcgaattt tccgcgttgc
    14521 cccggttttc actaaatcac gtaactgcga aagatcgggt tgttcgacaa ataagagtaa
    14581 tgatggatcc tcttttaacc acgccgtttt cacatcggca gacagctgtg gattattaat
    14641 agccagcgac cgatcctgag gctcagtcaa tgtcgttaac aacaacgccg gggtatgtgc
    14701 gttgcgcaat gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca
    14761 ttcatcatct gatttatctt ttatcgtagc cagtaccgca aaaaatacat ggagatcttc
    14821 actgatattt atatcaacct tgatgaatta tgaatatacc ggaaatcgaa aaaatcgcag
    14881 caaaggaaat attattatgg gtttatgaga ggatatttca gaaaaatgcc ctcccgttca
    14941 aggggaaggc gaggaattac agatagcgac acagataaga ggtgggttcg gcaacttgca
    15001 gatgaaactc gctgtgaccg ggaacattaa acaccgaacc ggcttcatac acttgccagt
    15061 cggtcgcgtc aggcagtaac acattcagcg cgccactgat taccgtcatc tcttccggct
    15121 cagcggtgct gaaggtgtat tcgccttcaa ccataacacc cacactggcg cgaccagtgc
    15181 tgctgctgga aaagccgatc gatttcactt tgccggaaaa gtactcatta ctttgaagca
    15241 taaactggcc ctgtctggta gttgaatatc tacttcacta taggggccag aggcgctgac
    15301 tgtcacgcaa aattacacca gtaattccgc agccagccgt gcaatcaata cgttagacag
    15361 caagacagga acatcgagct gtttttgcag taaatcgcga tgacgttggt gaaatcctaa
    15421 acaatccagc atgatgacat cagccccttt tgccagtaat tctttcccgg catcaatgat
    15481 tttttgttct gaatcatgaa tggggttacc caatgaaaat accggcgatt tctgcaaaat
    15541 ttgccatttt tgcgcctgaa cgggcagcat ctcctcaacc ggaacgataa cccccacttg
    15601 atgatcttca acaatagagg aaaccagcgg tggcaatatc cgcgacggct caagaaagat
    15661 cgtattacgc gcagtcatac tactaatgtt tgccgtactc attaatataa tgacgtcgta
    15721 accccgatta tcgagtactt caaccacacc ttgcaggtca cgctccactt tgcgacgcga
    15781 aacatgggcc agctggttgt catttaataa tgtgagaatg gtgtcttcgc ctgcttctgg
    15841 cgcgtactcc gccatcactt cttcacgact taacttcccc agcaggctat gatgggaaat
    15901 attatcttcg tcaatgtatt ccgtcaggag cggcaaaact tcctgcatag gtacaatgcc
    15961 gatggtcagg atcgccaacg acgcactcat tgttcatcca ccttactttt ctttcgtcgt
    16021 taattaccgg gcaagtgtga agcaccactg ctgacactta cttgtgctac aaatgacaaa
    16081 aagcgtagca gcagacgtgc acggcataat gtaaagattc caaatgattc cagtaatgga
    16141 ttgttattgt ttaatattct aattattaga aaaacatgaa ttatgaaaaa tgtgacgcag
    16201 atcccttgta gtggcactca gaatcccttc cgagcagtct gatgcgatca ctctttgtca
    16261 gggtcctcat agcgtttttt cgcatccatc gcttcctgag cggtcggatg ttcactgatc
    16321 aacgagtctg gtttaggatg atcggctctg agttggtacc aggttaccgt ctgtcctggt
    16381 tttccttttt caatcgtcac tatatatgct tcacgaggat aaggcggttt cgttggcatc
    16441 gttcttcctt atttcacggg atgaacgtta agtataggcg ctcgaaaatc aacaattgat
    16501 cgtctgtgcc agggcgctac gaatttcaga aatcacctgg ctgggttcgt ttgttgcgtc
    16561 gatgataata tgcgcaactt cgcgatatag cgcatcgcgt tcttccagca cttcctgaac
    16621 ttcttcgctc agcggttttc ccgttaaggt tggccgtaaa tcttcttccg gtgcagcttg
    16681 cagtcggtta accaggactg atactggcgc acacaaataa accacgatcc cgttattttg
    16741 catgaagtga cgattaaatt ccgtcagaat aatgccgccg cctgtagcga taacggtgga
    16801 tggcgcagtt accgcttcca gcgccgccgt ttctctggcg cgaaatcccg cccactcttc
    16861 cctttcgacg atctccgcga ccgtcatatt gagctgtgat tgcaaccact gatcggtatc
    16921 gacaaaccga cggttaagcg aatcggcaag ggccattccg accgttgttt taccacagcc
    16981 ccgaggcccg atcagaaaaa gaggttgtgt catcgtgggt tttccccaat aggtcgcaat
    17041 gcggcgaaag ccggtgtcat gagaatagcg atcataccat caaactagta caatttcgat
    17101 tgtaaaggaa aaattccact taaagtgaaa atctcaatac accccttact ataccaataa
    17161 ataatcaaga atgaagtgta aataataaat tacatttggc cgcgactacg ttgcacttcc
    17221 agccaccatt tctccagctc tgcagcaaag gcctggcggt cacgttgtga aagactatct
    17281 ggtccgccgg tctggatccc actggcacgt aaggtatcca taaaatcgcg catcgtcagg
    17341 cgctcacgaa tggtcgctgg cgtgtaacgt tcgccgcgcg gattaatcgc cgcagcgcct
    17401 ttctcgatgg cttcagcagc caaaggtata tctgcggtga tcaccaaatc gcccgcttca
    17461 cactgccgga caatttcgtt atcggcaacg tcgaaacctg ccgcgacgcg cagcgtacga
    17521 ataaatcgcg atggcggcac gcgtaaactc tggtttgcta ccagtaccag cggcatctgc
    17581 atacgttccg ccgcgcgata caaaatctct ttaattacat tgggacacgc gtcggcatcc
    17641 acccaaattg tcataaagtc atcctttgtt gggtaatcct ctattgtgtc gcgcttttgc
    17701 cttccggcat agttctgttt atgcttctgc cagcgattat caaaacaatg aatttcacgg
    17761 caggagtgag gcaatggaaa agaaaatcgg ttttattggc tgcggcaata tgggaaaagc
    17821 cattctcggc ggtctgattg ccagcggtca ggtgcttcca gggcaaatct gggtatatac
    17881 cccctccccg gataaagtcg ccgccctgca tgaccagttc ggcatcaacg ccgcagaatc
    17941 ggcgcaagaa gtggcgcaaa tcgccgacat catttttgct gccgttaaac ctggcataat
    18001 gattaaagtg cttagcgaaa tcacctccag cctgaataaa gactctctgg tcgtttctat
    18061 tgctgcaggt gtcacgctcg accagcttgc ccgcgcgctg ggccatgacc ggaaaattat
    18121 ccgcgccatg ccgaacactc ccgcgctggt taatgccggg atgacctccg taacgccaaa
    18181 cgcgctggta acccccgaag ataccgctga tgtgctgaat attttccgct gctttggcga
    18241 agcggaagta attgctgagc cgatgatcca cccggtggtc ggtgtgagcg gttcttcgcc
    18301 agcctacgta tttatgttta tcgaagcgat ggccgacgcc gccgtgctgg gcgggatgcc
    18361 acgcgcccag gcgtataaat ttgccgctca ggcggtaatg ggttccgcaa aaatggtgct
    18421 ggaaacggga gaacatccgg gggcactgaa agatatggtc tgctcaccgg gaggcaccac
    18481 cattgaagcg gtacgcgtac tagaagagaa aggcttccgt gccgcagtga tcgaagcgat
    18541 gacgaagtgt atggaaaaat cagaaaaact cagcaaatcc tgatgatttt tgccggacgt
    18601 caggccgcca cttcggtgcg gttacgtccg gctttctttg ctttgtaaag cgccaaatct
    18661 gccgatttca accactcgcg atagtgactc atttgtgggt tcagcggcgc aacccccaca
    18721 ctaatccgta aagttacctg tggcgtattc ggcaagcgta acgtgtttag cccttcatgc
    18781 acccgtaaca tggcggtaat ggcgctctca gctggcgtac cggacatgat taccgcaaac
    18841 tcatcgccgc caaaccgacc aatcacatcg ctaccgcgca gggtaatttg taactgtcgg
    18901 gtaagcgcca caatcgcttc atcgcccaca tcatggcccc aggtatcgtt gatgctcttg
    18961 aaatggtcga tatcgataat cagtaatgtc gcatcgcgat tatgccgccg acagttatca
    19021 aattcattgc gtaacatagt ttcccaatga cgtcggttat acacgcccgt cataccgtcg
    19081 cgggtactca tgacctgcaa cctgcgttta tgttccgcca gtttagtcgc cgtctggtag
    19141 ctgacccagc caaacagcag aggataaatg acgataatgg gaagggagag ccaccattcc
    19201 agcggcgcac tattgaacga cacggtgatg cccgtcagtt cgagggtgac aaggcaggaa
    19261 accaccatca acaccagacc cgcgacaaac agacgggggc cgcctgcccc catcaaattc
    19321 agacacataa tcatcaacat cgcggtggaa ggcagcacgt ttacgcccat tacgcctacc
    19381 cacattcccg ctaatactgc atcggttttt aagttgtaaa tttcccggct aagcggatcg
    19441 acggccctgc tcgctatctg ccaggctaaa tgcggccaga cgaacgccca gccgaccaac
    19501 accagccacc accagcccgg cggcgggtgc gaaaccagcg ttgaagcaat cggtaagaac
    19561 atgccagcca ggccaaccgc acggggtagt ctgacgcgac gggcgaagcg cagcccggac
    19621 cgctgatgtt cgttttgagg agttaaagga ggttcctccc catgcgccgc cgcttttttg
    19681 aaaaagtttt catcattcat tatttttggg aacattcaga aacaattttc ccaaattata
    19741 gagacggggc taaaggcaga agtatgaaat ttcgggtgag ccactttcgt agctcacccg
    19801 ttagcactat caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat
    19861 catcaccttt cagagattgt tgcgttgctt gattattaca ttcgcgcatc ttttgctgct
    19921 gtggcgtcaa acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt
    19981 aggtcttacg agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca
    20041 tacgctgttg ttgcggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca
    20101 gtccgaaaag caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt
    20161 aaccatgagc gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac
    20221 cacccgacag cgaaaattca ccgccgggcg cggttttatt tcagccccag ggcggctttc
    20281 atggtgtaga agagatcggt ctggtcggtc agtccaacga cattggcggc atgtgggcca
    20341 tacgccgcaa tacgcagctg actaccggta tgttcttgtg aatcctcttc ggagttcccg
    20401 taactcatca ccatcactgc gccatctttg gtatttagcg cctgggtgag gcccggcgct
    20461 ttggtgtccg gcgcaacaat ctggctggcg tgggcgtgat cagcggtgac tatgaccagc
    20521 gtgttgccat cctttttagc gaattccagc gcccgttgta cggcttcgtc gagatcgacc
    20581 gtctcgccaa tttgcccaca aggattcgca gcgtgatcct gtttatcgat tgatgcacct
    20641 tcaacttgca ggaaaaagcc tttctcattt ttactcaaca attcaatggc tttatcagtc
    20701 atctgcgcca gggtcggtac gctgtcatta cgttgcggat taggcgtaca ggtaactgcg
    20761 ggcttgtcga tattgccgtg gtacgttgct ttcggtcctt gccagcgcac tggcatattg
    20821 ccgtcagcaa acagtcctag caggggtttt tgctggttcg cttccgtcac cgcattcagt
    20881 gaggcagcat cgctcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt
    20941 ccctgccatt caccggcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta
    21001 acatcggcac gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttctagc
    21061 gcgttacccg gacatttttc actggtcgcg ctgggaccgt agcatttgcg cgaggtcaca
    21121 tgcgccacca gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc
    21181 gccagacctg cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg
    21241 acgcccagcg cgccgttata ggttttgaca ccggttgacc aggcggttgc tgacgcagcc
    21301 gaatcggtga cgtagtccgg tttgccggtt tttttattca gcgcatagtg agtgtattgc
    21361 ccggtaagcg gtaatgcatc gataccttta aaaaagccgc ccgcaccttc ggcataatta
    21421 cgtgcggcag taatttccga atcccccatc ccatcgccaa tcagcaaaat aatatttttt
    21481 gcaggtttat cgctaagaga atcacgcaga gcggcggtct gatcacccgt taagcggcga
    21541 gcaccgccgg gtgcagtaat atcgccctga gcagcccggt tttccagaac aggcatttct
    21601 ggtgtccggg cttttgtcac aggggtaaac agtaacggta agagtgccag tgcaatagtg
    21661 ctttgtttca ctttattttc tccatgtaca aatacattaa aaaataaaaa caaagcgact
    21721 ataagtctcg gccgtgacaa ctttatgaca gctgttgaaa agattaactt tttactgacg
    21781 aggatgcttc aataacttct ttacgtaatc gcgcagcagc tccgtatcgt cgtcaggaat
    21841 gctggcatcg ggctttacct cgtacagcgc cccctctacc tgatcaatca accgctgttg
    21901 gtcattttgc gccatattgc gaagcattgc agtgacgata atctccaaag cttctacctg
    21961 cgcacacagt tctttcgact cttcttcttt ttgggcaagc ttaaataaca actcagcaat
    22021 gagatttttc atgtctgtat ttccttatcc aaagtatgga gaagttaaca ttatgtttca
    22081 caacttcata gtgtctggaa gtattc
//
LOCUS       DBANUU010000079        21850 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000079 DBANUU010000000
VERSION     DBANUU010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.079"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(9..1994)
                     /gene="yjcS"
                     /locus_tag="ACIE68_002923"
     CDS             complement(9..1994)
                     /gene="yjcS"
                     /locus_tag="ACIE68_002923"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFX5909204.1"
                     /translation="MNNSRLFRLSRIIIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            complement(2267..3196)
                     /gene="alsK"
                     /locus_tag="ACIE68_002924"
     CDS             complement(2267..3196)
                     /gene="alsK"
                     /locus_tag="ACIE68_002924"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0019316 - D-allose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HFX5909205.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPG
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPHQVV
                     RFIAASSSDFNGAQGAAILAHQHFLPQSCAKAP"
     gene            complement(3180..3875)
                     /gene="alsE"
                     /locus_tag="ACIE68_002925"
     CDS             complement(3180..3875)
                     /gene="alsE"
                     /locus_tag="ACIE68_002925"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HFX5909206.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLATKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RCHDMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTTQIL
                     AAKSEVQPHAKTA"
     gene            complement(3886..4866)
                     /gene="alsC"
                     /locus_tag="ACIE68_002926"
     CDS             complement(3886..4866)
                     /gene="alsC"
                     /locus_tag="ACIE68_002926"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HFX5909207.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGV
                     DPFLAAMIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            complement(4845..6377)
                     /gene="alsA"
                     /locus_tag="ACIE68_002927"
     CDS             complement(4845..6377)
                     /gene="alsA"
                     /locus_tag="ACIE68_002927"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HFX5909208.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNISYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLVHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCCPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMMSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            complement(6504..7439)
                     /gene="alsB"
                     /locus_tag="ACIE68_002928"
     CDS             complement(6504..7439)
                     /gene="alsB"
                     /locus_tag="ACIE68_002928"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HFX5909209.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEAFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKILVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVNAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            complement(7498..8388)
                     /gene="alsR"
                     /locus_tag="ACIE68_002929"
     CDS             complement(7498..8388)
                     /gene="alsR"
                     /locus_tag="ACIE68_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose utilization transcriptional regulator
                     AlsR"
                     /protein_id="HFX5909210.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            8747..9196
                     /gene="rpiB"
                     /locus_tag="ACIE68_002930"
                     /gene_synonym="alsI"
     CDS             8747..9196
                     /gene="rpiB"
                     /locus_tag="ACIE68_002930"
                     /gene_synonym="alsI"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HFX5909211.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEVDGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
     gene            9265..9594
                     /gene="yjdP"
                     /locus_tag="ACIE68_002931"
     CDS             9265..9594
                     /gene="yjdP"
                     /locus_tag="ACIE68_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFX5909212.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(9741..10499)
                     /gene="phnP"
                     /locus_tag="ACIE68_002932"
     CDS             complement(9741..10499)
                     /gene="phnP"
                     /locus_tag="ACIE68_002932"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFX5909213.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(10501..10935)
                     /gene="phnO"
                     /locus_tag="ACIE68_002933"
     CDS             complement(10501..10935)
                     /gene="phnO"
                     /locus_tag="ACIE68_002933"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFX5909214.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHNAHRFYLREGYEQSHFRFTKAL"
     gene            complement(10922..11479)
                     /gene="phnN"
                     /locus_tag="ACIE68_002934"
     CDS             complement(10922..11479)
                     /gene="phnN"
                     /locus_tag="ACIE68_002934"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFX5909215.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(11479..12615)
                     /gene="phnM"
                     /locus_tag="ACIE68_002935"
     CDS             complement(11479..12615)
                     /gene="phnM"
                     /locus_tag="ACIE68_002935"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFX5909216.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            complement(12612..13292)
                     /gene="phnL"
                     /locus_tag="ACIE68_002936"
     CDS             complement(12612..13292)
                     /gene="phnL"
                     /locus_tag="ACIE68_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFX5909217.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(13469..14227)
                     /gene="phnK"
                     /locus_tag="ACIE68_002937"
     CDS             complement(13469..14227)
                     /gene="phnK"
                     /locus_tag="ACIE68_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFX5909218.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMAIGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(14224..15069)
                     /gene="phnJ"
                     /locus_tag="ACIE68_002938"
     CDS             complement(14224..15069)
                     /gene="phnJ"
                     /locus_tag="ACIE68_002938"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFX5909219.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            complement(15062..16126)
                     /gene="phnI"
                     /locus_tag="ACIE68_002939"
     CDS             complement(15062..16126)
                     /gene="phnI"
                     /locus_tag="ACIE68_002939"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFX5909220.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSIAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            complement(16126..16710)
                     /gene="phnH"
                     /locus_tag="ACIE68_002940"
     CDS             complement(16126..16710)
                     /gene="phnH"
                     /locus_tag="ACIE68_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFX5909221.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(16707..17159)
                     /gene="phnG"
                     /locus_tag="ACIE68_002941"
     CDS             complement(16707..17159)
                     /gene="phnG"
                     /locus_tag="ACIE68_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFX5909222.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(17160..17885)
                     /gene="phnF"
                     /locus_tag="ACIE68_002942"
     CDS             complement(17160..17885)
                     /gene="phnF"
                     /locus_tag="ACIE68_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFX5909223.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(17906..18685)
                     /gene="phnE"
                     /locus_tag="ACIE68_002943"
     CDS             complement(17906..18685)
                     /gene="phnE"
                     /locus_tag="ACIE68_002943"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFX5909224.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(18791..19807)
                     /gene="phnD"
                     /locus_tag="ACIE68_002944"
     CDS             complement(18791..19807)
                     /gene="phnD"
                     /locus_tag="ACIE68_002944"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFX5909225.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(19832..20620)
                     /gene="phnC"
                     /locus_tag="ACIE68_002945"
     CDS             complement(19832..20620)
                     /gene="phnC"
                     /locus_tag="ACIE68_002945"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFX5909226.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(20753..21196)
                     /gene="yjdN"
                     /locus_tag="ACIE68_002946"
     CDS             complement(20753..21196)
                     /gene="yjdN"
                     /locus_tag="ACIE68_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFX5909227.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(21356..21691)
                     /gene="yjdM"
                     /locus_tag="ACIE68_002947"
     CDS             complement(21356..21691)
                     /gene="yjdM"
                     /locus_tag="ACIE68_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFX5909228.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         4735 a         5846 c         6085 g         5184 t
ORIGIN      
        1 gagtgacatt aatttggggt tacgatattc acccacaaat cgaaattatc cagacaggcg
       61 ataatttctt ccagtttcgc gccattgcca ataattttgg ctttcttcgc ttttaccaga
      121 tccgccattt tggcttgccc ggtcagcacg gcgtgcagat cttcacggct gatgtagaaa
      181 gaggcgtcag cttgggattg cagtgtttta cggtagttaa gcacgctatc gttcagcgtc
      241 aggttgaggt tatcgccatt gctcatattg aagttcaggc tgatattttt acccgcggct
      301 ttcgcgctat cgaggcgaac ggacataaag tcgaacagca tttcgaccga catcccgcga
      361 atggtgtccg gggaaccggt ggtgccgtgg ctgaacttat gcaccccttc gcgcagctct
      421 ttcgcgccgg tcaggtagaa accgcgccag gtggcggact cggcctgata gcccagctgt
      481 tcaaagttat tcgcttgcag attcttcgcg acctggtcac ccgggttggc ggcaatcacc
      541 tgtttcagca gttctgccga ccagcggtaa tcaccttgct tgttcgcttc ttgcgccagg
      601 ttgatgacac gggcagaacc gcccagcgcc tgcacgtaac gtttacccat ctccacctga
      661 ccatacggat gcaggttagc cgggttaccg tcgtaatagc caagatagaa gttatacacc
      721 gcgcgggcgt tgtggctgac agaaccgtaa tagccacggc tggcccagtt attggcaagt
      781 gcaggcggca gtttaatcat gtcgccgatt tcattcatgg tgtagccctg gttcgccagg
      841 tgcagggtct ggtcgtgaat gtacttgata gtatcgcgat atttaccaat ataatcattg
      901 atatgcttat tgccccagac cggccaggtg tgcggcataa acaggacttc cgcgtcgtta
      961 ccccacatat ccagcgtttc gttcagatac tcggtccact tgctggtgtc gcgggttttc
     1021 gcgccgcgta gagtgtagaa gttgtgcagg gtatgcgtgg cgttctcggc ggtacacagc
     1081 gctttcaggg ccggaatata gaagtgcatt tcggctggtg cttcgctacc tggggtcatc
     1141 aggaagtcaa actccaggcc gtcgataatc atcttctcac cggttttggt gatggttttg
     1201 ttcggtgcga tgatgctcgg gtagcccgat gccagcgtta cgccaagacc attgccgatg
     1261 ttaccctgag ggttatgcgc cagcagcagg ccgtaggagt aaagtgcacg gcggctcata
     1321 atattacccg ccagtacgtt ttcgctgatg gcttcgtcca taaagccagc aggggcgata
     1381 acctgcactt taccggattt cacatcggct tcagagataa tgcctttcac gccgccatag
     1441 tggtcggtat ggctgtgggt ataaataacc gcaacaatcg gtttttgcgg gcgattttgg
     1501 aagtaaaggt caagggcggc ttttgcgctc ggtggcgtca ccagcgggtc gatgacaata
     1561 atcccggtat caccttcgat aaaggtgatg ttggagatat cctgaccgcg aacctggtac
     1621 attctgtcgg tgactttaaa caggccggaa ataccgttta actgcgactg acgccacagg
     1681 ctagggttaa cggtttcggg cgcgggcgca ttgatatcaa atttataatc ttccccgcga
     1741 taatatggtt tgccattagc atcgcgcaga ataccttcat ccagcagcgg agcgataaat
     1801 ccacgctgag cgtcgtcaaa atcctgacga tcgctaaacg gtaatgattt ggcgtaattc
     1861 tgattaacct gttgggtata ttgagtggcg gctttcgctt catctgttgc gtaagcggta
     1921 tttaccatca tgccgctggc ggcagttaac gcaataataa ttctgctcaa acggaataac
     1981 cgagagttat tcatataaat ctccatttat catttatgtc gtaaatatgt aatccatgca
     2041 aaatacgtca tttaaagctg gggttatcat aattgggaat ttgcgacttt aataagtgga
     2101 agtgtgagct gaacgatcca ttttattagg catttgtgct gaagaggaaa agttacagta
     2161 aaactatcat tgcagaaaat aatatgagcc gttttatgtg tttattatat atattgtacc
     2221 tgtcgttggc acagcgggac attgcgctac tgtgccaacg ctttactcat ggggctttag
     2281 cacaggactg tggcaaaaaa tgttgatgcg ccaatattgc tgcaccctga gcgccattaa
     2341 agtcagaaga tgaggcggca ataaagcgca cgacctgatg cggcagtgga cggcgcaggt
     2401 acttttgggt catggcaatg agagtctcgc gtgggaaggc gggcatatcc atcacaccac
     2461 cgcccagaat caccgcatcg ggatcgaaca gattaatgct ggtggcaatg gcccgtgccg
     2521 cgttttcaag cagactctgg acgaaagggg cgttttccgc atggacgaaa aggtcgctca
     2581 atgggtaatt tcggggctgt tgttcgtacc agcgtcttag cgccattcca gagcaattgg
     2641 tttccaggca cccaggattg ccacacgcgc agtgttgggt catatctccc agggggatat
     2701 gacccagttc gcctgccaca ccgtgtgcac ccgtccacgg cgcaccgttc atccacactg
     2761 cgaaccccat ccccgtaccg agataggccg ccagaaccag ttgttgcgta aggcggtttt
     2821 ctactacgtc ccaggagagt tgcaggttaa cgtcgcggga aaactcaacc ggacaattca
     2881 gcgtattttc gagcttatcg gcgagatcat ataaatccgc cgctgttaac ggcaggttag
     2941 gcgtagaaat tatggtgcgt ttatctttac tgaccagcgc cggaaatccc atcaccagac
     3001 catgacagcg agcgttaaag cgcctgagtt gctcgtcaat catttcgccg atacccgaca
     3061 ccaggccggg agcaatgact tctgcggtcc gctttttttc gcagtgtagc gtttcacctt
     3121 ctgctgtccg cagacaaaag cggatatgcg ttgcccccat atccacgccc gctacgacgt
     3181 tatgctgttt ttgcatgagg ctgtacctcg ctttttgcag ccagaatctg cgtggtcata
     3241 attctccatg cttcgtcgat attttccgca tgattaaaca ggccggaagt gccgacgata
     3301 aagacatccg cccctgccgc catcagtttt tcgtaagttg cctggttgca ggaaccgtcc
     3361 acctcaattt cgtactccag accttctcgt tcacgccatg ccttcagttc ggcaagttta
     3421 tccagcattt caggaatgaa cggttgtccg gcaaagccgg gatcgacagt catgaccgta
     3481 attttatcgg ccttatggat atagtatttc atggcctcaa ctggtgtctc cgggttaagg
     3541 atcagcccca ctttcatgtc atgacagcgg atttcatcaa tcaggcggaa cgcctgaccg
     3601 ttgatagttt ccggatgcaa agtgatgaaa tcagctcccg cacgcgccag ttgagcaatg
     3661 taatcctgcg gccgcgtcac catcagatga cagtcgagcg gtttagttgc cagtttttta
     3721 acctgactta cgaagaacgg tgagagtgtc agattgggga caaagtgacc atccatgata
     3781 tcgatgtgaa agtaatcggc atggctgtcg ataaattcga tctgttcttt aaatttcagc
     3841 agatccatac acattaacga gggggagatt ttcatgttca attccttact tactgataag
     3901 acggtcaagg gcgacagccg cgataattaa tccgcccatc accaccagtt ggtaataggt
     3961 ttgtacctgc aaaatattca gaccgttgtt gatggtgccg atgatcaacc cgccaatcac
     4021 cacagagaaa atgcgcccct tgccgccgaa gaaactggtg ccgccaatga tggcgctggc
     4081 aatggcatag gtttcaaaac ccataccggc aagcggttct gcggcaccga gtcgtgcagt
     4141 tgagacgacg cctgccagac ctgcacaaac accggagatg ataaacacca ccaggatgtg
     4201 gaatttcacg tcaatcccgg aatagaacgc cgaatttttg ttgccgccca gtgcgtagat
     4261 gttgcgcccg agccgcatac gcgttgtcag aaaccaaagg atgagcgcga caattagcga
     4321 gaagataacg gggacaggta tcccaattac gctggcggca aagaagttca cgaagtcaaa
     4381 ggagaagccg tataccgagt tggcatcgga gatcaccagc gtgatcccac ggaaaatcgc
     4441 gttggtgcca agggtgatga tgaacgggtg tagccccgtc cagttgacca ggcagccgtt
     4501 gatcgccccc agtgcgccgc caaccagtac accgccaatc atcgctgcga gaaacgggtc
     4561 aacacctgcc aacatcagtt tggcggtcac cataccggaa agcgccagaa tcgcgccaac
     4621 cgagaggtcg ataccagcga ccaggatagc gaaaaactcg cccatgccga tcaataccgt
     4681 cacggagctt tgaacaaaaa tctgggtaat attattggtg gtcagaaaat attctggcga
     4741 cagcgaacca aaaatggcga cgatgatcgc caggataaaa aaggtgccgt atttatccca
     4801 gaacagcgca aagttgaacg gtttcttctc gctcgcttcg ctttttactc ttgtggtaaa
     4861 gcccatgcca taatctcctc ttcgctcatg tcatcgcgat tcgtcaggat ttgcgtcagt
     4921 cgtccttcgc agaacacggc gatgcggtcg cagacggtga taatttcagg tagttcagat
     4981 gacatcatca ggatgacttt tccgtcgtcc gccagttggc gcatcacttt gtaaatttcg
     5041 gctttcgcgc caacgtcgat gccgcgggta ggttcatcga aaataatcac ttccgggcaa
     5101 cagcacagcc atttggagat caggactttc tgctgatttc ccccggagag ttcggtgata
     5161 ttctggttta ccgaatgaca tttcagcgcc agcagttcgc gttgattttc agcggtacgt
     5221 tgctcgtcaa cttcatgaaa caagcccatc gcgcctttat agccgccgtc tttcagactg
     5281 cggctgatcg ccatgttctg agcgatggaa aagttgggaa aaaaaccgtt atcccggcgg
     5341 ctttcagtga tgtaagccat cccttttttc actgcatcca ggggggaacg tggagagata
     5401 tctttgccat taagacggat ttctccgcca gcgcgtttat caacgccaaa cagacaattc
     5461 atcagttcag tacgtccgga accgaccagc ccggcaaagc ctaatatttc tccccggcag
     5521 acgctaaatg agatatcccg gacctttttt ctgtcacggc tggtgacgtt gcgcacctca
     5581 aaaaccgtat cgtgcacaag gttgctgaca ttctccttca tcgcgttaaa acggttttgc
     5641 agttcgcggc ctaccatcag acggacgata tcgtcatttg acacatcgct taccatgccg
     5701 ctgcaaacgc tgctgccgtc tttcatcacc gtatagcggt cgcaaatacg gcgaatttcc
     5761 gccaacttat gcgagatata gacgatggcc gtaccctctt tacgcaactg attcatgatc
     5821 agaaacagat agtccacctc tttattggtg agtgaggagg tgggttcatc catgatgatg
     5881 actttggcat cgagcatcag cgttttggca atttccagca tctgcttgtg gctgatagat
     5941 agattcgcca ctttctcatc taaatcaact ttcaagccca cgcgtaataa catcatggcg
     6001 gcgcggacac gcatttctcg ccagtcgata atattgacgc cacagatttt tttcgtcaga
     6061 tgacgaccaa tatataaatt ttccagtacg gttaattcat caataacgct gagttcctga
     6121 taaataatcc cgataccgag ttgtgccgct aatttatgat ccagcttgtt atagctaatg
     6181 ttattaatgg taatggtgcc tttggtcggc tcatgtattc cggataaaac cttcattagc
     6241 gtggatttac ccgcgccatt ttctcctagt aatgcatgta tttcaccagg ataaaccgtt
     6301 aaattaaccg actttaatgc gtgaaccgga ccaaaggact tgccgatccc cgccatcgat
     6361 atatatggcg tggccataaa taacctctct gcacgaaata tcgtattatt gcctgatggc
     6421 tacatttatc aggtctacgt tcaggttatt ttgttggccg gataagacac ttcattagca
     6481 tcatcatccg gcatcattca gttttattga gtgaccagga ttgaatcgac cagtttaaat
     6541 tccggtgctt tatccagcgg gataaccttg ccggatttct cagcgttaac catcagcttc
     6601 agacccgttg cgccaatatc tgccgggttc tgcgcaaccg tcgcggtcat ttgtccggct
     6661 tccaccattt tgcgggcttc cggaattcca tctgtaccga cgaccaatat tttgcccgtt
     6721 tttccggcgt ttgcgactgc ctgagcaaca cccattgcca tcgtgtcatt cgcgcaatag
     6781 atcgctttaa tattcggatt acgttgcaac acgttagtgg cgacatccag tgctttaatg
     6841 cggtcccagt cagcgggctg gctggcgaca agcttgatct ggcttgcttt tttgaaggct
     6901 tcggtggcac cattacgacg cgcttcaccg gaggcgttac cggctttacc ctcaatgatt
     6961 gcgacttcac caccttcagc gcccaatttg tcaataatga acgacgcgcc tttcgccccg
     7021 acagcaacgt tatcggtggt gacaaaagct tccacattgc cgccagcttt tttcagatta
     7081 tccatgtcga ttttttcatc gagattaacc agataaatgc cttttttcca tgcgcgggcg
     7141 acaggcatga ccagattcac tgaggataat ggagcgaagg cgataccttt gtaattttta
     7201 ttactgagat cttcaaataa ctgcaattga gattgaaaat cgccttctga aggagaggca
     7261 aaaatatcaa cgctgacgcc cagtgttttt gcttcatctt caatgccttt tttcatatct
     7321 acccaaaatg ggttggagag ggttttcaat acgacagcat attcggcggc agcaaaagcg
     7381 ctggttgaca acattaagcc cacgagtgtg ccgctgaaat atttcagata tttattcata
     7441 ataatgttct cacggtaagg ggacaggtaa ctccgcgatg gcggaattac ctttttctta
     7501 tttcagcgcg cctggtgaga agaaatcaac aattgcgccg gttttttgca tattaatatt
     7561 agcttgttca atgttgagct gggcgacaga gacaaaaaaa gcgtccagca aagttagttg
     7621 taatattctt gccgaggcat tacgacctaa taacggcgtt tccggggctg gtgagcaaat
     7681 aatataatcg gccagtttcg ctatcggtga atggtagcta tgggttatac aaataatctt
     7741 tgccccgttc ttttttgcca gttctacggc cgcttttaca tcactggttc gcccggaatg
     7801 ggtcactacc agcacaacat ctccttcctg taacaacgaa gcggacatca tcatgatgtg
     7861 agcatcagga taggcctgac agcgtacgcc aatgcgcaag aacttgtgct gtacatcagc
     7921 acagatagca tttgatcctc cggcaccgta caaatcccgc tgtctggcct gatagaaaaa
     7981 gcgggcggca cggtggatct catcaacgtt gacgatcgac tgaccttcca taatggtgcg
     8041 taaagtgatg ttaaatacct tattcaccac atcctgcggc gcttcatcaa aagccaactc
     8101 ggaaggcaat acctgttctg actgagaaaa ataatcttcc agcgcactgc gtaagttacg
     8161 aaagccgcta aaccccagca gctttgatac cttaactatc atcgcttcag ataccgccag
     8221 agcttctgcg acatctttaa ttgcgggtgc acaactcagg ttaccgggtt tgagtaacca
     8281 ctctacgatg cggctttcat tttctgtcat tccttcctgc ttcattcgca ggtaaggcgc
     8341 taaccctata ccgttcggaa gcgctgaatc aaactctgac tggctcatca cgttctcttg
     8401 tccttgaatt gaagacgcca ctatatcacc acagcaagaa cagaaaagat gctggcgact
     8461 tcacaaaaaa ataaaattta taaagtttat tttattgtga acaatggcga gtggcttaaa
     8521 attttatttc atttaattag tttaaaaaca tgatgttatt tgtttttctg gttttttaga
     8581 tgatagatgt tgctggatgg tgagtgtctt taacgctatt gcgctgtcag atgtaactct
     8641 gtaaaacaga tcaggaaggc gtaattcatc gattttgggt gtttttaatt cattaacatc
     8701 acaaatgttt tttgattgtg aagttttgca cggacgggga agatgaatga aaaagattgc
     8761 atttggctgt gatcatgtcg gtttcatttt aaaacatgaa atagtggcac atttagttga
     8821 gcgtggcgtt gaagtgattg ataaaggaac ctggtcgtca gagcgtactg attatccaca
     8881 ttacgccagt caagtcgcac tggctgttgc tggcggagag gttgatggcg ggattttgat
     8941 ttgtggtact ggcgtcggta tttcgatagc ggcgaacaag tttgccggaa ttcgcgcggt
     9001 cgtctgtagc gaaccttatt ccgcgcaact ttcgcggcag cataacgaca ccaacgtgct
     9061 ggcttttggt tcacgagtgg ttggcctcga actggcaaaa atgattgtgg atgcgtggct
     9121 gggcgcacag tacgaaggcg gtcgtcatca acaacgcgtg gaggcgatta cggcaataga
     9181 gcagcggaga aattgagatt catccactac ttgcatggat gagtaatgac taatgtggat
     9241 agagtttctt tttgaggttg gctaatgaaa cgctttccgc tatttctttt attcaccctg
     9301 ctcacgctgt ccaccgttcc ggcccaggct gatattatcg acgatactat tggtaatatt
     9361 cagcaggcga ttaatgacgc ttataaccct gaccgtgggc gagactatga agattcgcgc
     9421 gacgacggct ggcagcgtga agtgagcgac gatcggcgca gacaatatga cgaccgccgc
     9481 cgccagtttg aagatcgccg caggcagctg gacgatcgcc agcgccagct tgatcaggag
     9541 cgtcggcaac tggaggatga agagcggaga atggaagatg agtatgggcg atgaggttgg
     9601 gtatggagca ggcatcgccc ctcaccccaa tggggagagg gagaaaacga gcgcaatatt
     9661 caatattgag cacaatcggt cccctcgccc ctctggggag agggttaggg tgagggggcc
     9721 agcgcgcaca ttccctctca tcacgccacc ccaatctcca tcccatcaaa ccccacctca
     9781 aaccctgacg gtagtgcgtt ttccatcagc cacgcatcaa actggtggct gatatgggtc
     9841 agaatcacct gtggcgagcg gataacctga ttcagcgcaa gcacggtatt taaatcacag
     9901 tgattacgcg gtgcatccgc gcgcggcggg tgactgcaat caatcaccat cacctgcgga
     9961 tgattattga gtaaaaattt cagcgttttt tccggcaggc cagcggtgtc agacagccac
    10021 gccacccggc tatgtgccgt ttccagcaga taaccgaagg tcagttttga gtggttaagc
    10081 ggcagcggcg tgacctgcaa cccctgcaaa tcaaacacca caaacggttc cactgtgtgg
    10141 ctgaaatcaa gcaggcccgg atgtttaaac agatcgtcgc agccctgttc atccggcggg
    10201 ccgtagaccg ggatcacatc gcccacgccc cagcgtagcg gaaatagccc ctggacgtga
    10261 tccatatggt agtgcgtcag caaaaactgc tggaacgatc caggcgacca gcgatcggtg
    10321 agatcgtgcc gcccggcgtc gatcagggtg atcgcgtcgt taaacttcac tacgccgctg
    10381 catggctggc ggcgatactg cggcgagcgc cgcgctctgg cgcaggccgc gcactcgcag
    10441 ccccatgccg gaacgccctg tgcgccgcca gtgccggtga gcgtgagggt caggctcatg
    10501 ttacagcgcc ttggtgaagc ggaagtggct ctgctcgtag ccttcacgca ggtaaaaacg
    10561 gtgtgcgttg tggcgcttca cgttggtgga aagctcggtc atttcggccc cggcctggcg
    10621 ggcttcttct tctgcccatg ccagtaattt actgccgacg ttcagaccgc gcgcctgcgg
    10681 catcaccacc agttcttgaa tttcgccgat ccagttgaca tgatgcagat gaaactgcaa
    10741 atgcaggccg atcatgccga caacttcgcc atccagcagc gccagatggt agcgcatgtt
    10801 tgggtcgcgc aggttggcgt taaagcccac gcgaaacgca tggtggtcaa actccgcctg
    10861 ttttagctca caaatcagcg cgtaaaccgc gtcggtatcg tactgcgtgg ccgggcgaag
    10921 ctcacaagca ggcatggtgt ttctccttct gatggatcag cgtcagcagc gtgtcgaccg
    10981 actggcgcag gctgccgtca ttgttgagcg tatggcaatc ctgtggagta tagcgggcgg
    11041 cgcgcgccag gcgggcgtta atttcactgg cgttttcacg gccacggttt tccaggcgct
    11101 ggcgcaggat ctccggcgaa acctgtaaac agacgggcag cagcgccgat tgatagcgcg
    11161 cccgtgcctg cggcaaatgc gcgcgtgaac cgttgacgac tacgtcgaat ccagcgtgca
    11221 gccagaggtc aatctcgatg ccgacgccat aatacagacc gttagcgtgc cagcttaagg
    11281 ccagcaggtt ttgccccgcg cgggtaaaaa actcccgctc actcagggcg atatggtttt
    11341 cacttccggc gctggcgtcg cgcgtgatgt agcgatgcgc caccagtaac tgcgtttgtt
    11401 cccgcaggcg gagttccgcc agcaggctgt ctttcccgga gccggacggc cccattaacc
    11461 aaatcagttt tcccatcatc agaacaccct tttaccctga cgccagacgt ggtcgatatg
    11521 aatgtgattg ccctggcgat gcgccagcac caggtcagcg cgtttaccct cgccaatcac
    11581 cccgcgatcc tgaagattca gcgcctgcgc cggatttttg gtcaccagcc tcaccgcctg
    11641 cggtagcgta aagcggttgc tctcgtcatc ggcgacgcgg aacgccgcat ccagcaggct
    11701 ggcggggtag tagtcggaag agaggatatc cagcagacca agctgcgcca gttcactggc
    11761 cgccacattg ccggagtgcg agccgccacg cacgatattt ggcgcaccca tcagcacgtt
    11821 catgccatgc ttacgcgagg cttccgccgc ttcgaacgtg gtgggaaatt cggcgatcac
    11881 gctgccaagt tggtgggatt cggcaacgtg ggcgtgcgtg gcgtcatcgt ggctggcgag
    11941 cgcaatttgt cgcgcgcggc acagggcggc gatggattcg cgattcggct gcgaccagcg
    12001 tgcggcgagc gccaattgct cttcttcgta ctgctgcatc tgcgcatcgg tgagggagta
    12061 tttgccctga taatattcgc gatacttctc gcggttagcg aactggcgct ggcccggtga
    12121 gtggtccatc agcgacacca gcgtcaccgg ttcgcgctga accagttttt caaacagcgg
    12181 cagtgtggtg tgatgcggca gttcgcagcg caggtgcagg cggtgctcgg cgcggttgac
    12241 gccgcgtttt tgcgtctctt cgatggcgtt gatcatcttc tccagattct ccagccgatc
    12301 gccgccgtcg cgcacatcac caatcgccac ggcgtccagt acggtggtga tgccgcttgc
    12361 caccatcagc gcgtcgtggc tgctcatcgc cgaatgggcg ggccagtcga ctttcgggcg
    12421 cggggtgaag aatttatcga ggttgtcggt atgcagctca atcaggccgg gtagtagcca
    12481 gccaccttcg ccgtccatcg cttctggcag gcggctctgg ctttcggcaa aggcgcggat
    12541 ttcgccatcc tgcacctcca gcgaaccgtg caccacctcg ttttccagca ccagcttaac
    12601 gttattgata atcatgaaga ggctcccatt gggtgcaggc ggtcggcgac gtcattacgt
    12661 acagcttcgt catggaagat gccgacgatg gctgcgccac gggctttggc ttcgtgaatc
    12721 agttccacca ccgcggcgct gtttttggcg tccagcgagg cggtaggttc gtcgagcagc
    12781 agaatggggt agtcgacgat aaagccgcgg gcgatgttga cgcgctgctg ttcgccaccg
    12841 gaaaatgtcg atggtgccag gtgccacagg cgttccggca cgttcaggcg ggtgagaaga
    12901 cgcgcggctt tagcggcgca ggcttcacgc ggaacgcctg tatcgagcag cggctgcatc
    12961 accacttcca gtgctgagat acgcgggatg acgcgcagaa actggctcac ccagccgacg
    13021 gtggttttgc ggatttccac cactttgcgc gctggcgcgg tgaccaggtc tacccactcg
    13081 tcaccgtgtt tgatctggat ttgaccttcg tcgggtagat agttggcgta cagcgagcgc
    13141 agcagggttg atttgccgct gccggagtga ccgtggagca ccacgcattc gcctgcgttg
    13201 acggtgagcg aggcgcgttg taagacgggc aggcgcacgc cgttttgctg gtgcaggatg
    13261 aaggttttac tgacgttttg tacgttaatc atttttcgcc tcgtggttcg catattgggg
    13321 agcggtgcgc gcattgcccc tcaccctaac cctctccccg aaggggcgag gggaccgatt
    13381 gtgcgcggtg ttgaatattg cgccggtttt ctccctctcc ctccgggaga gggccggggt
    13441 gaggggatca gcgcgcacgg tgttgcattc aattctgcaa aaccgatgac accagcagtt
    13501 gtgtatacgg atggtgcggg tcgtcgagca cgcggtcggt taacccactc tccaccactt
    13561 gcccctgctt catcaccagc aaacggtccg ccagcaggcg ggcgacgcct aaatcgtggg
    13621 tgacaatcac taccgcgagg ttcagctcca ccaccaggcc gcgcagcagg tcgagcaggc
    13681 gtgcctgtac cgacacatcc agtccgccgg tcggctcgtc cataaacacc agcttcggat
    13741 gcgtcaccag gttgcgggcg atctgcaaac gttgctgcat gccgccggaa aaggtggtcg
    13801 gcaggtcgtc gatccggttg gcgggaattt ccacctcttc cagccacttc tgcgcggtgg
    13861 cacgaatatc gccgtaatga cgtgccccga tcgccatcag ccgctcgccg atattgccgc
    13921 ctgccgacac ctggcggcgc aggccgtcga gcggatgctg atgcactacg ccccattcgg
    13981 tgcgcagcag gcgacggcgg tcggcctcgc tcattgcgta cagcgaacgg ctctcatagt
    14041 gaatctcccc ctgctgcggc gtcaggcgcg cggagattga tttcagcagc gtggtcttcc
    14101 cggagccgga ctccccgaca atgcccagca cttcccccgg ccataaatca aaagagacat
    14161 cgctaaagcc tttgcccggc gcgtaaaggt gggtcaggtt attgaccgaa agtaacggtt
    14221 gattcattgg cttttcgcct cgctctgttg gcggcaataa tcggtatcgg agcagacaaa
    14281 catgcggttt ccggcgtcat ccagcaccac ttcatcaaga tagctgtggg tcgatccgca
    14341 gatggcgcat ggctcatccc actgctgaac ggtgaacggg tgatcgtcga aatcgagact
    14401 ttccacgcgg gtaaacggcg gcaccgcata gatgcgcttc tcgcgccctg cgccgaacag
    14461 ctgcagggcg ggcatcatgt ccattttcgg gttatcgaat ttcgggatcg gcgacgggtc
    14521 catcacgtag cgcccgttca ccttcaccgg ataagcatag gtggtggcga tatgaccgaa
    14581 gcgggcgata tcttcataca gtttcacctg catcacgccg tactcctcca gcgcgtgcat
    14641 agtgcgggtc tccgtttcgc gcggctcgat aaagcgcagc ggttccggga ttggcacctg
    14701 gaagataatg atctgatctt cggtcagcgg cgtttcgggg atgcggtgac gcgtctggat
    14761 aagcgtcgca tcgtccgtac gttcagtggt gtttaccccg gtcacgcgct taaagaagtt
    14821 gcgaatcgac acggcgttgg tggtgtcatc cgcaccctgg tcaatcacct tcagcacgtc
    14881 gctttcgcca atcacgctgg cggtgagctg tatgccgccg gttccccagc cgtatggcat
    14941 cggcatctcg cgcccgccaa acggcacctg ataaccgggg atcgccaccg cttttaagat
    15001 ggcgcggcgg atcatgcgtt tggtctgctc gtcgaggtag gcaaagttgt agccgctcag
    15061 attagccatg gttcttctcc tgttgcagac gtttgagtag ctccagttcg gcctggaaat
    15121 cgacgtagtg ggggagtttg aggtgcgaga caaagcctgc ggcttcgacg ttgtcggcat
    15181 gtgccagcac aaactcttca tcctgcgccg ggcctgtcgc gtgctcgccg tattccggag
    15241 cctgcaacgc acgatcgacc agcgccattg ccatcgcttt gcgctcgctc atgccgaata
    15301 ccagcccgta gccgcgcgtg aagtgcggcg gctcatccgg cgggtcgata aaaccgttga
    15361 ccatttcaca ctcggtcatc agtagttcgc cgacgtttac cgcaaatccc agctcttccg
    15421 gcacaatcga cacatcgatg taaccactgc ggatctcgcc cgcgaacggg tgattgcgtc
    15481 cgtaaccacg ctgggtggag taggccagcg ccagcaaata gccttcgtcc ccgcgcatta
    15541 attgttgcag gcgggaagaa cgtgaacagg ggtaaaccgg cggcgtgcgg gtgatgtcat
    15601 ccggctgtgc tccgctatcc tcttcaaact tcgccagccc ctgacgcgcc agcaggctga
    15661 aaacgtgcgg cgacggctgt tgttcgctgt cggcggtggt cagcgtcggc gcttcgccgt
    15721 ttgccagcag agtgaaatcg agcaggcgat gggtgtagtc gtaggttggg ccaagcagct
    15781 ggccgccggg aatatcttta taaaccgctg aaatacgccg ttcgagacgc atctccgtgg
    15841 tgtcgagcgg ctcgcttacc gccagtttcg tcagcgtggt gcggtaggcg cgcagcagga
    15901 aaattgcttc aacgttatcg ccgctggcct gtttcagcgc cagcgccgcc agttcgcggt
    15961 cggcaatgcc gccttctgtc atcacgcgat ccaccgcgag gttaagctgc tgttcaatct
    16021 gggcgatgct cagttcgggc aaatcggtat cgccccggcg tcggctctct tgcagggcgt
    16081 gggcggcgtc gatcgccttt tcgccccctt tcacggcaac gtacatcagc acacctccac
    16141 atgagtggtt cgcgggatag ccagcaggcg ctcgccgcag gtcaggatta ggtcgatgcc
    16201 gagcgggaac gggtgcgggc gctcggtgag ttcgtgcaga atgcactctg gcagctgcgg
    16261 agcgatcatt cgttcttcgg caatgcccgc gccggtgaga cgcaacatgc gtccgccgct
    16321 caggctggcg acctgtaaaa tcagcgtcgc gcccgcttcc ggcgcaacgg cggtgccggt
    16381 ggaaagggcg ttgagctgtt cgctggaaat cgcctcatcc gtcaccgcga aggtcgcctg
    16441 ttccggctgg ctgaccagcg gcgcgttggt atgaaaacgc aggctctgat tgacgatatc
    16501 gttacttaat ggcgtggcaa gccacaccgg cgtgtcgtta tcggccagcg tcagcagcac
    16561 gctggtggtg gcgatattca gcggctgcca gccgcgtttg agctgatgca gggcgacaat
    16621 cacgcccggc tcgctcatcg cctttaacag gcgacgaaaa ctgtgctggg catcctgcac
    16681 gggaagcata aaagcggttt ccagggtcat gcgttgtctc cgcgaaccat cgtaaagaag
    16741 tcgacccggc tggcgttcac ttcggcctgg cgtgcggcaa tgcgtgccat acggtcagcg
    16801 tccagcgggg caataagggt ttctgataag ttttgaaaat agcgagattg ctgcatcagc
    16861 gcgtcaatca gcgcgcagcg ttcggcgtgc tgtttgtcgc gccccagcac ccagctgtag
    16921 ccgagcgtgc cgtcagtcag gcgtacggcg gcgcgggtga gcgtggcgtc gccggcaaaa
    16981 aagcgttcgc cggtgccgcc catccgcgcc tgaatctgca ccaggccggt ttcggcggcg
    17041 cggataacct catagtcggc ggtgatgttc agtgctttca ggcgggctgc cagttcagcc
    17101 ggttggctgt gcgccagtac ggacatccag tgctggcggg tcgcggtatc tgcgtgcatt
    17161 cagtgctcca tagtgaattc aatcatgtcg gcgcgcgtca ggctgacgga gtactccgcc
    17221 gggctgcttt caccgtcacg gtggttaagg gtgcgcacgc acagcagcgg cgacatattc
    17281 ggaatttcaa gacgctggca ctctttggcc tgggcgcgac gggcgctgat ccgcgtctgg
    17341 ctgcggcgca gcgcaattcc ggtttgctcg cgcagaaaat cgtgcagcga accgctgtcg
    17401 aagcgttgca gcgtcggcca aagggtgagg tccgcaaagt agtggtcgat taaacagagc
    17461 gcgacgccgt tgacgcgacg cagggtgcgc aaatggataa cgttctcgcc ctcggtaatc
    17521 cccagcgcgt cagcgacgtg accggacgcg gggcgcaata ccgacagcag tttttcgctg
    17581 gtgggatggc taccctggtc cagcaggttc tggctaaaac gcgcctgggc gttgagcggg
    17641 taatcgaacg ggcgcatcag caccagcacg ccgacgccct gacggcgctg tacccagcct
    17701 ttttccacca gttggtcgat ggcgcggcgc agggtgtggc ggttcacctc aaagcgcgcc
    17761 gccagttgct gctcggcggg aagatagtcg ccgcagcggt agtgttgacg aagctcctgc
    17821 tcaagttttg cggctatctc ctgatagcgt gttgggtagc tggtcggatg tgtagacaag
    17881 tgcatagata tcaatgcctc gcttatcaga taaagtgctt acgcaaccgt tgagagagga
    17941 aatccagcag gctgaccgtg acgatgataa gcaccatcag ggcgcaggtt tgttggaact
    18001 ggaaaccgcg aatcgcttcc cacagggtga cgccgatccc gcctgcaccg accatgccga
    18061 cgacggtcgc cgagcggacg ttggattcga agcgatagag ggagtaggag atcagcagcg
    18121 gcatcacctg cggcagcacg ccgtagagga tctcttcgag cttgttggcg ccggtggcgc
    18181 gaatgccttc caccgggccg ggttcaatcg cttccaccgc ttcggaaagc agtttggaga
    18241 gcacgccggt ggtgtggata aacagcgcca gcacgccagc gaacgggccg aggccgacgg
    18301 cgaccacgaa cagcatggcg aagaccattt cgttaatggc gcggcaggcg tccatcaggc
    18361 ggcgaacggg ctggtaaacc caccacggca ccaggttttc ggcgctcatc aggccgaagg
    18421 ggatggagag aaccaccgcc agcgccgtgc cccagacggc gatttgcagc gtgacggcca
    18481 tttcggtgag gtaatcctgc cactggctga aatcgggcgg gaagaagtcg gcggcgaacg
    18541 tcgccatgtt gccgccgtct ttaatcagcg tgagcggggc catttccgcg ccctgccacg
    18601 agacgaccaa cacggcgaga acaacggccc agctcagaag cgagaaccag ctgcgcttgg
    18661 gtggggcgat ggtgatggtt tgcatgtttg gctccggtct gtaggccgga taaggtgctt
    18721 gcaccgcatc cggcatcaac gcctgcacat tgtcggatgc gacgccgggg cgtcttatcc
    18781 gacctacgat ttactgcacc gctttactca ccgaactcat cgcgcttaac gcgttgttca
    18841 ggcggtccag gtcatccagc tgcgcctgaa tctcggtggt ttttgccagc ttgtcctgct
    18901 cattcagtcc tttattgctt ttcacgccct gcatctcttt aaacagcgcg agctggcgaa
    18961 tcggcaccag ttgcaggtcg ctggaagcgc ggaacggcgc ccagcccagg cgttccagca
    19021 ccgctttttc ttccggcgtt ttgccgtagt tcataaagaa gtcgtagatc ttgtctttgg
    19081 tggtttcgga aaggttttta cgccagacga tcggatcgcc tgggatcagc ggcgacttcc
    19141 agatcacctt cagttctttc agcttctctg gcgcggaggt tttcagcttg tcgaggtttt
    19201 cggtgttgtt ggtggcaaca tccacctgct tgttggcgac ggccagcgcg ttggtttcat
    19261 gcccagcgtt gacggtgcgc ttaaagtcgc tggcggagat attgtttttg gcaaagacgt
    19321 agtaaccggg gacgaggaag ccagaggtgg agttaggatc gccattgccg aaggtgagat
    19381 ctttccgctt cgccagcaga tcgttcaggt tgttgatcgg actatctttg ttgacgatca
    19441 acacgctcca gtaacctggc gatccatccg ccgcgaccgt ctgggcgaag acctggccgt
    19501 tggcgcgatc caccgcttcc attgccgaca ggttgccgta ccaggcgata tccactttat
    19561 tgaagcgcat tccctggata atgcctgcgt agtctggggc aaagaaggcg ttcaccttca
    19621 cgcccagctt cttctccata tcctgtaaga atggcgtcca ttgcggtttc aggttttgct
    19681 gtgattccgt tgaaataatg ccgaaattca gcgccttttc ctgctcttcg gcgtgtgccg
    19741 ggcttaacag ggtgctgagg ctgaacatgc tggtgaaggc cagcgaggca attatcttag
    19801 cgttcattcg ttttcctcaa tgatggggat gtcaggcagc tttcgcgttc tcttcgatgc
    19861 gattaatgct gcggtagaga tggtcaaaac gttcgttatc aaactgttgg ctgctgccgt
    19921 cgtagaaaac gtgcccctgg cgcagggcga cgatgcgttc gcagtagcgc agggcgtaat
    19981 ccacctgatg cagcgtgacg accacggtga tgccgtcgtt ctgattgatg tcgcgcaggg
    20041 tgtccatcac gatgcgggcg gattccgggt ccagcgaggc gatgggttca tcggccagaa
    20101 tcaccttcgc ctgctgcatc agcgcgcggg caatcgccac acgctgctgc tgtccgccgg
    20161 agagggtgga aacgcgctga tgggcaaaat gcaccatgcc aacgcgggtc agcgcctgta
    20221 acgcgcgctg tttctgctcg ccggtgaacc agctaaaaca ggtgcgccag aacggcgtgc
    20281 tgccgagcgc gccaatcagc acgttctcca gtacgctcag gcggttcacc aggttgaatt
    20341 gttggaagat gtagccggtg ttggcgcggc ttttgcggat atcgcgcgcc agacggcctt
    20401 cgcgctggac tgtgcggccc agcagctcga tatggctgcc ggcggattta tcgccggtaa
    20461 tcaaaccgct taagtgacgt aaaagggtgg atttgccgga acccgacggc ccaagcagag
    20521 ccaccatttc accgtgatga atgttcagat caaccgcatg cagcgcctga tgctgattga
    20581 aggttttggc gagcttctcg acacggataa tcgtttgcat gatgcagcct ccctaaaaaa
    20641 gtggctccat cgtggcggat tattgtgacg atttggttaa ttaaaagtga ctaacagatg
    20701 aagagttaac gggaattcga tgacagtgcg ggagggcggg cctcccggcg ggttattgcg
    20761 ttggttgttg tttgacgaca ttaatcatcc acggtacgcc aaatttatcg gtgactttgc
    20821 caaagccatg cgcccagaaa gtttcctgcc aggccatttc gatttttccg ttagcggcaa
    20881 gattgtcaaa ccagcgtttt ccttcttcga cctgttgcga atcgagcacc agcgtaaagc
    20941 cggagtagct ggcttttcct gacggtatag catcgctcat catgatgtcg cttccggcaa
    21001 tgcgcacgtt ggcgtgggcg atggcggtat cgggaaattg cattccggaa gggcagttct
    21061 cggcgctgtc ctgcgctgat tttggcattt cgccgaagct gattttatag agcagttcag
    21121 cgcccaacgt acgttgataa taagcaattg cgtcggcaca gttaccggca aaagagaggt
    21181 agggacttaa cggcatgatg gtgacctcag ttaagagaag ccagttaagt gtagttcgaa
    21241 atttatacag atgagagaga agcctgataa gacgcggtca gcgtcgcatc aggcagtcgg
    21301 catcgttgcc ggatgcggcg cgagcgcctt atccggctta ccgatcacaa tacaatcagt
    21361 tctttttcac aaactcagat ttcagtttca tcggaccaaa accgtcgatt ttgcaatcga
    21421 tgttatggtc gccttcaacc aggcggatgt ttttcacttt ggtgccaatt ttcagcatcg
    21481 aagagctacc tttcaccttc agatctttaa tgatggtaac gctgtcgccg tcagccagca
    21541 gattgccgtt agcatcttta acgatcagct cgtcgctttc ctgtgcaggt tctgcgtcgt
    21601 tccattcgta ggcacattcc gggcagatgt acatgccgtt atcttcgtaa gtgtattcgg
    21661 agttgcattt tgggcagtgt ggtaatgaca tgtggatttc ctcaaaagtc agcagggcaa
    21721 aagcgccaaa aaacggcaga ttgccgaaaa aggccgcaat tatacacaaa atccctgagt
    21781 ttgtcggggg tactgctgat tttagacgac gatcgttttc atttgaaagg aaacgcaaac
    21841 agaaaataat
//
LOCUS       DBANUU010000080        21292 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000080 DBANUU010000000
VERSION     DBANUU010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.080"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(36..1334)
                     /gene="eno"
                     /locus_tag="ACIE68_002948"
     CDS             complement(36..1334)
                     /gene="eno"
                     /locus_tag="ACIE68_002948"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFX5909229.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="ACIE68_002949"
     CDS             complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="ACIE68_002949"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFX5909230.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="ACIE68_002950"
     CDS             complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="ACIE68_002950"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFX5909231.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="ACIE68_002951"
     CDS             complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="ACIE68_002951"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFX5909232.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="ACIE68_002952"
     CDS             complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="ACIE68_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFX5909233.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4810..7044)
                     /gene="relA"
                     /locus_tag="ACIE68_002953"
     CDS             complement(4810..7044)
                     /gene="relA"
                     /locus_tag="ACIE68_002953"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFX5909234.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRRRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="ACIE68_002954"
     CDS             complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="ACIE68_002954"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFX5909235.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8450..11206
                     /gene="barA"
                     /locus_tag="ACIE68_002955"
     CDS             8450..11206
                     /gene="barA"
                     /locus_tag="ACIE68_002955"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFX5909236.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLTMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="ACIE68_002956"
     CDS             complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="ACIE68_002956"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFX5909237.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGNGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAKRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12799..14139)
                     /locus_tag="ACIE68_002957"
     CDS             complement(12799..14139)
                     /locus_tag="ACIE68_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFX5909238.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="ACIE68_002958"
     CDS             complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="ACIE68_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFX5909239.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15928..16377)
                     /locus_tag="ACIE68_002959"
     CDS             complement(15928..16377)
                     /locus_tag="ACIE68_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFX5909240.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16395..17177)
                     /gene="truC"
                     /locus_tag="ACIE68_002960"
     CDS             complement(16395..17177)
                     /gene="truC"
                     /locus_tag="ACIE68_002960"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFX5909241.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17177..17506)
                     /locus_tag="ACIE68_002961"
     CDS             complement(17177..17506)
                     /locus_tag="ACIE68_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFX5909242.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18128..18673)
                     /gene="syd"
                     /locus_tag="ACIE68_002962"
     CDS             complement(18128..18673)
                     /gene="syd"
                     /locus_tag="ACIE68_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFX5909243.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18741..19589
                     /gene="queF"
                     /locus_tag="ACIE68_002963"
     CDS             18741..19589
                     /gene="queF"
                     /locus_tag="ACIE68_002963"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFX5909244.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19701..21065
                     /gene="ppnN"
                     /locus_tag="ACIE68_002964"
     CDS             19701..21065
                     /gene="ppnN"
                     /locus_tag="ACIE68_002964"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFX5909245.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5210 a         5712 c         5067 g         5303 t
ORIGIN      
        1 ggaggctggc atttttaaat cagataaagt cagtcttatg cctggccttt gatctcttta
       61 cgaccgttgt acggtgcttt ttcgcccaga gcttcttcga tacgaatcag ctggttgtat
      121 ttagcaacac ggtcagaacg gctcatagaa ccagttttga tctggcctgc agcagtacca
      181 acagccaggt cagcgatggt agcgtcttca gtttcgccag aacggtgaga gataactgca
      241 gtgtagccag catctttcgc catcttgatt gcagccagag tttcggtcag agaaccgatc
      301 tggttgaatt tgatcaggat ggagttagcg ataccttttt cgataccttc tttcaggatc
      361 ttggtgttgg ttacgaacag gtcgtcacca accagctgga ttttgtcgcc cagaactttg
      421 gtctggtatg cgaaaccgtc ccagtcagat tcgtccagac cgtcttcgat agaaacgatc
      481 gggtactgtt tggtcagttc ttccaggaag tgagtgaatt cttcagaggt gaacgctttg
      541 ttgccttcgc cagccagaac gtatttacca tctttgtaga attcagaagc tgcgcagtcc
      601 atcgccagag tgatgtcttt gcccagttca taaccagcag ctttaacagc ttcagcgata
      661 acagccagag cttcagcgtt ggaacccagg ttcggcgcat agccaccttc gtcaccaaca
      721 gcagtgttca tgcctttcgc tttcagaact tttgccaggt gatggaaaac ttcagaaccc
      781 atgcggatgg cttctttcac agttttcgcg ccaaccggct gaatcatgaa ttcctggata
      841 tcaacgttgt tgtcagcgtg ctcaccaccg ttgatgatgt tcatcatcgg aaccggcata
      901 gagtatttgc ccggagtacc gttcagttca gcgatgtgct cgtacagcgg cataccttta
      961 gcagctgcag cagctttggc gttagccaga gatacagcca ggattgcgtt cgcgccgaat
     1021 ttggatttgt tttcggtgcc gtccaggtcg atcatgatct tgtcaatgcc agcctgatct
     1081 ttagcatctt tgccaatcag cgcctgagcg atcgggccgt ttaccgcagc aacagctttg
     1141 gttacgcctt tacccaggaa acgggatttg tcgccatcgc gcagttccag agcttcacgg
     1201 gaaccagtag aagcacctga cggagcagct gccataccga cgaaaccacc ctccagatgt
     1261 acttcggctt caacagtcgg gttaccacgg gagtcgatga tttcacgacc gatgattttt
     1321 acgattttgg acattaggtt ttcctcaagt cactagttaa actgaaactc cagacaaaca
     1381 acgcgtaccc agggtacgcg ttgccgctct aactttttta cttacttcgc ctgacgtttc
     1441 tggaactcgc tggcggcttt cacaaagcct gcaaacagcg ggtgaccatc acgtggagta
     1501 gaagtaaact ccggatggaa ctggcaagcc acgaaccacg ggtgattcgg aacttcgatg
     1561 atctcgacca actgatcatc cccggaacgg cccgcaacgc gcagacctgc atcttcaatc
     1621 tgtttcaaca gcatgttgtt gacttcgtaa cggtgacgat gacgctcaac aattgtcggc
     1681 gcattgtaca gctggcgaac caggctatcg tcaaccaact ggcactgctg tgcgccgaga
     1741 cgcatggtac cgccgagatc gctcttctcg ctacgaactt caacgttgcc gttttcatcg
     1801 cgccactcgg taatcagcgc cacaaccggg tacttacagt ctggcacaaa ttccgtagag
     1861 ttggcgttct ccatgttggc aacatggcga gcgtaatcaa ttaacgccac ctgcataccc
     1921 aggcaaatgc ccagataagg aatattgttc tcacgcgcaa aacgcgcggt cgtaatcatg
     1981 ccttctacgc cacgatagcc gaaaccgcca ggtacgagga ttgcgtccag acctttaagg
     2041 atttcaacgc cgcgcgtttc aacatcttgt gaatcgatca gtttgatgtt gacgctgaca
     2101 cgattcttca gcccaccgtg tttcagtgct tcgatcactg atttataagc atccggcagt
     2161 tcaatgtact tgccgaccat accgatggtg acttcactta ccgggttcgc ttcttcgaag
     2221 ataacctgtt cccattcgga cagattcgct tccgggcagt ttaagctgaa tcgtttacaa
     2281 atataatcgt ccagcccctg agatttcaac aggcccggaa ttttatagat ggaatcgacg
     2341 tctttcagag aaataaccgc tttttccgga acattacaga acaatgcaat cttcgcacgt
     2401 tcgttcgccg gaacagcgcg atctgaacga caaatcagga tgtcaggctg gataccgatg
     2461 gagagcagct cttttacaga gtgctgagtc ggtttggttt tgacttcacc agacgctgcc
     2521 atgtacggca ccagcgtcag gtgcataaac agagtgtgct cacggccaat ttcaacagcc
     2581 atctggcgaa tcgcttcgag gaacggcaag gattcgatat cacctactgt accgccgatt
     2641 tctaccagta ctacgtcatg accttcgcca ccttccagca cgcgctcttt gattgcgtta
     2701 gtgatgtgcg gaataacctg cacggttgcg ccgaggtagt caccgcggcg ttctttacgc
     2761 agaacgtcag agtagatacg acccgtggtg aagttgttgc ggcggctcat tttggtacga
     2821 atgaaacgct cgtagtgccc caggtccagg tcggtttcag cgccgtcttc agtaacgaac
     2881 acttccccgt gttggattgg gctcatagta cctggatcga cgttgatgta aggatccagt
     2941 ttcatgatgg tcacattgag gccacgggct tcaagaatgg ctgcgaggga ggctgcggca
     3001 atgcctttac ccagagagga tacgaccccg ccggtcacaa aaatatagtt cgttgtcatg
     3061 ctgaacctga gaagttaggt tgaaaagacg atggaataac caggacggga aagcagtata
     3121 cccgaacatg acctgtgcca caaactttca ttatccctcc tcttcgccag cgcactattg
     3181 aaatcaggag tgagaaaata gcccctttgg ggtaaatgtt tttgacgcaa atcaagcgct
     3241 tgtcatttaa aaaatgacac aaatggcgct tgaccgcgta attcccttag agatcaattt
     3301 cctgccgttt tacctgttgc cagacttctt ccattgtttc gaggtcaaca cctgtcattt
     3361 ccagtccacg cgcggcaaca atacgctcca cttcgcgaaa acgacgctca aatttttcgt
     3421 tcgctttttg caatgcgatt tccgcttttg tccctaaatg acgagccaga ttgaccgtgg
     3481 caaacagcag gtcccccatt tcctcctcca gtttagcctg gtcgacaaca gcctgccgcg
     3541 cttcgtacat cacctcgtcg atctcttcgt agactttatc gactaccgga ccaagcgtcg
     3601 tccaatcgaa gccaacgttg gcgcaacgtt tctggatttt ttgcgcacgc attaaagccg
     3661 gtaaactacg aggaatatcg tccagcgccg aatgctgcgc tttctgcgcg cgctcttcgg
     3721 ttttgatttg ctcccaacgg gcaagcactt cactactgtt ttcggcagaa ctatcagcaa
     3781 aaacatgcgg atggcgacgc tctaatttat cgctaatagc agcgcaaata tcattaaagt
     3841 caaagcgccc ttcttcctga gccatttgcg cgtaaaacac cacctggaat agcagatcgc
     3901 ccagttcacc gcgaagatcg tcaaaatctt cacgggcgat ggcgtccagc acttcgtagg
     3961 tttcttcaag ggtgtaaggc gcaatggtgg caaatgtctg ctctttatcc cacgggcagc
     4021 cgttttccgg atcgcgcagg cgctgcataa tagtgagcaa acggtcgatt tgattcattg
     4081 aattgtcctg aaaattgcgg gtctgtcagg tggaaacctg tgaccagaat agaagtgagt
     4141 tagtaacact acccaatcag tacgttaatt ttggctttaa tgagttgtaa ttcctctggg
     4201 gcaactgttc ctttcttcgt tgctcctctt gcccgccagg cgatactttt tacctgatca
     4261 gctaacgcta cgccatcacg ttcctgaccg gataaaacaa cttcgaacgg atatcctttt
     4321 gattgcgttg tacaaggaac acacagacac atacctgttt tgttgttgta catgaaagga
     4381 ctcaggacaa cagctggacg atgtccagct tgctcgctac cttttgtcgg gtcaaaatca
     4441 acccaaatca gatcgcccat atcgggtacg tatcggctta ccattaccag acttccttat
     4501 ctttcggctc tccccagtcg atattctcgt ggaggttttc cggcgtgatg tcgttgacca
     4561 gttcagcaag cgtaaatacg ggctctttac gcactggctc aataattaat ttgccatcca
     4621 ccaggtcaat cttcacttca tcatcaatat tcagattgag cgcctgcatt aacgtagccg
     4681 ggatccgcac cgccggtgaa tttccccaac gctttacgct actgtggatc ataacccttt
     4741 cctcaaaccg ctatcatatg tagatacagt atatatcaat ctacattgta gatacgagca
     4801 aatttctgcc taactcccgt gcaaccgacg cgcgtcgata acatccggca cctggttgag
     4861 tttacccagc acgcggccca gcacttgcag gttgtaaatc tcaatggtca tgtcgatggt
     4921 cgccagttgc tgtttggtgt cgctacggct ggcaacgcca agcacgttca ccttctcgtt
     4981 ggcgagaatg gtcgtgatat cacgtaacaa cccactacga tcattagcca ccacgcggac
     5041 caccagcgaa tatccggcgg agtagctctc accccatacc gcgtcaacaa tgcgttctgg
     5101 cgcatgggag cgcagttccg ccagttgttc gcaatcggcg cggtgtactg aaataccgcg
     5161 tccctgggta atgaagccga caatctcatc tccaggaatc ggctggcagc agcgcgcgat
     5221 gtggtgcatc aggttaccaa caccttcaac taccacacga ccgttatctt tactgcggtt
     5281 ttgcggtgtg tagctttttt gctgaagctg tttcagcgcg gcggcgtcct gctcttcggc
     5341 actcggctta ttaaattgcg attgcaggaa gttcaccatc tgattgagac ggatatcccc
     5401 gccaccaatc gccgccagca actcgtcgac atcattgaag ttgtaacgcg gcaacagatg
     5461 tttttctgct tctttcaggc tgatccccag atgttccagc tcgtcgtcaa ggatttgccg
     5521 cccagccaga atgtttttgt cacggtcctg tttacggaac caggcgtgaa ttttcgaacg
     5581 ccgacggctg gttgtgacgt aaccgaggtt tgggtttaac cagtcacggc tggggttcgg
     5641 ctgtttctgg gtgataattt caatctggtc gcccatctgc agctggtagg tgaacggcac
     5701 aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg acatcactgt ggatgtggta
     5761 agcgaagtcc agcggcgttg atcccgcagg caaatcaacg acatcacctt tcggcgtaaa
     5821 gacgtacacc cggtcgtcaa agacctgact acgtacttcg tcgagcattt cgccggaatc
     5881 agccatctct tcctgccacg caatcagttt acgcagccag gcaatccggt cttcatgtcc
     5941 cgaacgtgcg ccgccagcag ccgcgccctc tttatatttc cagtgcgcag caacacccaa
     6001 ctctgcatct tcatgcatct gtttggtgcg gatttggatc tcaacggttt ttccacccgg
     6061 ccccagaacc acggtatgaa tagactgata accgtttggt ttcgggttag cgacgtaatc
     6121 gtcaaactca tccggcaggt ggcgatagtg agtgtgcact atccccagtg cggcataaca
     6181 atcctgtaaa cgctcggcga caatacgtac cgcacgcaca tcaaacagct catcgaaggc
     6241 gaggttcttt ttctgcattt tacgccagat gctgtagatg tgtttcggac gaccatacac
     6301 ttcggcttta acgccttcag ctttcatctc agcgcgcaga tgaccgacaa actcttcgat
     6361 atagtgttcg cggtcgagac ggcgttcatg caacagtttt gcgatgcgtt tgtattcggt
     6421 cgggtgcaga taacggaagc agtaatcttc cagctcccat ttcagttgcc caatcccaag
     6481 acggtttgcc aacggcgcgt agatattggt gcactctttt gccgccagta cgcgttcatc
     6541 ttccggcgca tcttttactt cccgcagatg agcaatacgc tccgccagtt tgatgaccac
     6601 gcagcgaaaa tcatcgacca tcgccaataa catccggcga acgttatcga cctgttcgga
     6661 ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc gccgccatat cacgcacgcc
     6721 gtgaataagg ttaacgaccg acttaccgac gctctcacgc agcacatctt cgctgactac
     6781 gttggcatcc gccagaggga aaagcagcgc cgcccgcagc gtgtcaatgt ccatacttaa
     6841 tgtcgagagg atctccacca tctcaacacc acgccacaat aacagactgg catccggatg
     6901 cccctgcgtc tgttgcagac aatacgccca ggtttcggct aagcactcac acgacttctg
     6961 gctggtaata cccagacttg cgatccattt ttccggatca aattcaccag ccttattgat
     7021 atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg gaccagacct gccgaaatcg
     7081 gcaaatcgca actatttaac gcgcgagaaa agtaccatcg attccagatg tcccgtgtgt
     7141 gggaacatat ccagcatcgc cagtcgcgca atggtatatc ctgcttttaa taacgcttcg
     7201 ctatcccgag ccagcgttgc agggttacag gatacataaa ctatacgaat aggttccagt
     7261 tttataattt gctgcataac acctgcggca cctgctcgcg ccgggtccag caacacttta
     7321 tcgaagccgt ttttcgccca cggctgcttt gtgacatctt cttcaagatt ttcgtgataa
     7381 aacgtcacat tctgtaagcc attaagacgc gcattctgct ggcctttttc caccagcgcc
     7441 ggaacacctt ctacaccgac cacactggca gcttgtgtcg ccaatggcag tgtaaagttg
     7501 cccataccgc agaacagatc cagtacgcga tcttcaggtt gcacatccag ccattccaac
     7561 gcacgcgcta ccattttttg gttcacaccc gcattgacct gaataaaatc gcgcgggcta
     7621 aaagttaagc gcaacccgtt tgagtcatac cagggcatct caccagagac ggtttcgagt
     7681 atctcactat cgggggcgag atacagatcc aggccttcag aatgcgaaaa gcgttccagt
     7741 ttttcgcgat ctgccgaact tagcggtgcg gtatggcgca aaatcatcag cgtgccgctg
     7801 gttgcctgta ccagttcaac atgaccaaga tggcgcatag cttgtaagct gcccagacat
     7861 gccctgactt tgggcagcaa tgcttcaagt tggggcgcta aaatggggca ttgtttgacg
     7921 tcgacaatgt cactggagcc cgctttgcga aaccccatct gaagttgctg tgttttcggt
     7981 aagtagttca gacttaaacg cgcgcgacgg cgatagcccc agggaacatc ggcgatcact
     8041 tcagagacat cgtgtttcat taatcgggcg agtgccgcac ttttgcttcg ctgctgtaaa
     8101 tccacgctgg cgtgttgttg ctgacagcca ccgcatacgc caaaatgagg acagcgtggc
     8161 gtttcgcgtt ccgggctatc gcttaaccgg cgtacgactt tagcgcgggc atactgtttt
     8221 ttatcttcag taacagtaac ttccgcgttt tcctgcggca ataatccggg gataaatagc
     8281 gttttgccgt tatgtcgcgc cacgccctga ccaaaagagt cgaggtcgtt gactgaaacg
     8341 gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt agaattgcgc cattggcgag
     8401 actttctcaa tttaacagtg tgaccttaat tgtcccataa cggaactcca tgaccaacta
     8461 cagcctgcgc gcacgcatga tgattctgat cctggcaccg accgtcctta ttggtttatt
     8521 gctgagtatc tttttcgtcg tgcatcgcta taacgacttg cagcgtcaac tggaagatgc
     8581 cggtgccagc attattgagc cgcttgcagt ttctactgaa tatggcatga gcctgcaaaa
     8641 tcgcgaatct atcggtcagt taataagcgt actgcatcgt cgccattccg atattgttcg
     8701 cgcgatttcg gtttatgatg aaaataaccg actctttgtc acctccaatt ttcatcttga
     8761 tccctcatca atgcagctcg gcagcaacgt gccgtttcct cgccagctca ctgtcactcg
     8821 tgacggcgat attatgatcc tccgcacgcc gattatttct gagagttact cccccgacga
     8881 atcgcccagt agcgatgcca aaaatagtca aaatatgttg ggatatattg cgctggagct
     8941 ggatcttaaa tcggttcgct tgcagcaata taaagagatc tttatttcca gcgtgatgat
     9001 gctgttttgt atcggtattg cgcttatttt tggctggcgc ttaatgcgcg atgtaaccgg
     9061 tccgattcgc aacatggtga ataccgtcga ccgcatccgt cgcgggcaac tcgacagccg
     9121 ggtggaagga tttatgctcg gcgagctgga tatgctgaaa aacggtatca actcgatggc
     9181 aatgtcgctg gctgcttatc acgaagagat gcagcacaat atcgaccagg cgacgtccga
     9241 tctgcgtgaa acgctggagc agatggaaat tcagaacgtt gagttggatc tggcgaaaaa
     9301 gcgcgcccag gaagcggcgc gtattaaatc cgagtttctg gcaaatatgt cacacgagct
     9361 gcgtacacca ctgaatggtg ttattggctt tacccgcctg acgctgaaaa cagaattaac
     9421 accaacgcag cgcgatcacc tgaatacgat tgaacgttcg gcaaataatt tgctggcaat
     9481 tattaatgat gttctcgact tctcgaaact ggaagcaggt aagctgattc tggaaagtat
     9541 tccattccca ctacgcagca cgctggatga agtcgttact ctgctggcac attcttctca
     9601 cgataaaggg ttggaactga cgctcaatat taaaagcgac gtgcctgata acgtgatcgg
     9661 cgacccgctg cgattacagc aaatcatcac taacctggtg gggaatgcaa ttaaattcac
     9721 cgagaatggc aacattgata ttctggtaga aaaacgtgcg ctgagtaata ccaaagtgca
     9781 gattgaagtg cagattcggg ataccggcat tggtattcct gaacgcgatc aatcgcgctt
     9841 attccaggcc ttccgacagg ctgatgccag tatttcccgc cgtcatggtg gcaccgggct
     9901 ggggctggtg attacacaaa aactggttaa tgaaatgggc ggcgatattt cgttccatag
     9961 ccagccgaat cgcggttcaa ctttctggtt ccacattaat ctcgatctga acccgaacat
    10021 tattatcgaa gggccatcca cccagtgcct cgcaggtaag cgcctggcct atgtcgaacc
    10081 aaactccgca gcagcgcaat gcacgctgga tattttaagt gaaacgccgc tggaagtggt
    10141 ttatagccca acgttctccg cgctgcctcc cgcgcattac gacatgatgt tgttaggcat
    10201 cgcggtgacc ttccgcgagc cgctaacaat gcaacatgag cgattagcga aagcggtatc
    10261 gatgaccgat ttcctgatgc tggcacttcc ttgccatgca caagtcaatg ctgaaaaact
    10321 caagcaagat ggtatcggcg cgtgtctgct gaaaccatta acacctacac gcctgttgcc
    10381 tgccctgacg gaattttgtc atcacaaaca aaacacgctt ttgcctgtaa ccgatgaaag
    10441 taagctgaca atgacagtca tggcggttga tgacaacccc gctaacctga aacttatcgg
    10501 cgcattgctg gaagatatgg tgcaacatgt ggaactttgc gatagcgggc atcaggcggt
    10561 tgaacgggcg aaacagatgc cgttcgattt gatcttaatg gatattcaaa tgccggacat
    10621 ggatggcatt cgggcctgcg aactcatcca ccaactcccg catcagcaac aaacgccggt
    10681 tatcgcggta acggcgcatg caatggccgg gcaaaaagag aagctgcttg gcgcagggat
    10741 gagcgattat ctggcgaaac cgattgaaga agagcgattg cataatttgt tgttgcgcta
    10801 caagcctggc agcggtattt cctctcgcgt cgtgacgccc gaagtcaacg aaattgtggt
    10861 gaacccgaat gcgaccctcg actggcaact ggcactacgc caggcagcag gaaaaaccga
    10921 tttagcgcgc gatatgctgc aaatgttact cgatttcctg cctgaagttc gcaacaaagt
    10981 tgaggaacag ctggttggag aaaacccgga aggcctggtt gatttgattc ataaactgca
    11041 tggcagttgc ggctatagcg gtgtgccacg tatgaagaat ctctgccaac tgatcgaaca
    11101 acagctacgt agcggtacta aagaagaaga tttggaaccg gagctgctgg aactgttgga
    11161 cgagatggat aatgtcgcgc gcgaagccag caaaattctc gggtaatgga tgtcttgtcg
    11221 agtagcaatc ggctttttat gacgctggtt tcagattggt gacaaagtgc ctgtgtttat
    11281 gccggatgcg gcgttaacgc cttatccggc ctacaaaacc aattaaattc aatgaattaa
    11341 aaaatgatgt agacctgata catagcacaa caggctattt tgcgtttagc atcagtctca
    11401 aaccggctcc agatagagcc ggttttggtt ttctgtctta acgcaccatg cacgggcgct
    11461 tgttatcgaa cgtccagcca ggaatcagat actgcattcc catcgcatcg tcacgcgcgc
    11521 caagcccgtg tttctgatac agctcatggg ctttcatcac ttgatccata tcgatttcta
    11581 cacccagccc cggtttttct ggcacctgta ccagcccgcc tttgatctca aacggttctt
    11641 tggtcaggcg ctgattgcct tcctgccaaa tccagtgcgt atcaatagca gtaattttac
    11701 ccggtgcagc ggcggcaaca tgggtaaaca tcgccaggga aatatcgaag tggttgttag
    11761 agtgtgaacc ccaggtcagg ccaaattcat ggcacatttg cgccacacgt accgaacctt
    11821 gcattgtcca gaaatgcgga tccgccagcg ggatatcaac ggattgtaac gagagcgtat
    11881 ggcccatctg ccgccagtcg gtggcgatca tattggttgc ggttggcagg ccagttgcgc
    11941 gacggaactc tgccatcact tcacgcccgg agaaaccttg ctccgcacca cacggatctt
    12001 ctgcataagc cagtgaccct ttcaggtatt taccaatttt aatcgcttcg ttcagcgacc
    12061 aggcaccgtt aggatcgagc gtaatacgcg cctgcgggaa acgtttcgcc agtgccacaa
    12121 tagactcggc ttcttcttcc ccggccagta caccgccctt cagtttgaaa tcgttgaagc
    12181 catatttttc atatgccgct tccgccaggc gcaccaccgc atccggcgtc atcgcttctt
    12241 catgacgcag gcgataccag tcgcatgagt catccggctg gctttgatac ggcagcggtg
    12301 tggctttgcg attaccgacg aagaacagat aaccgagcat ttcgacttcg ctacgctgtt
    12361 gaccattgcc cagcagcgat gccacgttta cccccagatg ctgccccagc agatccagca
    12421 ttgccgcttc tatcccggta actacatgaa tagtggtacg caaatcaaat gtctgcaaac
    12481 cgcgcccacc agcatcacga tcggcaaaag tattacgcac cagcgtcaga acgtttttgt
    12541 attcacccag cgttttacct accaccagcg gaatcgcatc ttccagcgtt ttacggattt
    12601 tctcgccgcc gggaatttcc cctacgccag tgtgaccaga attatctttg ataatcacaa
    12661 tattacgcgt aaagaacggt gcgtgtgcac cactcagatt catcagcata ctgtcatgac
    12721 ccgccaccgg gatgacctgc atttcagtaa caacaggcgt cgtaaattga gaactcatat
    12781 ttatgtcctt tttcagaatc aatgacggcc gaaaacggga cgtttacggt caaaggtcca
    12841 gccggggatc aggtactgca tcggacctgc gtcgttacgc gcaccgccag gcagacgttt
    12901 ataggcctca tgtgcctttt gtacctgttc ccagtccagt tccacgccca gaccgggcgc
    12961 atcaggaacg gcaatttttc cgtttttaat ctccagcgga tttttggtca ggcgacaatc
    13021 gccctcctgc caaatccagt gggtatcgat agcggtagga ttacccggtg ccgccgcgcc
    13081 cacatgggta aacatcgcca gagagatatc gaaatggtta ttagaatggc agccccaggt
    13141 cagcccccag tcgtcgcaaa gctgcgccac acggactgca ccggaaagcg tccagaagtg
    13201 cggatcggca agtggaatat ctaccgcatt gagcatcacc gcatgaccca tttcgcgcca
    13261 gttggtggcg atcatgttag tcgcgacggg caagccggtc gcccgccgaa actccgccat
    13321 cacttcacgt ccggagaagc cctgttctgc gccgcatgga tcttcggcat aggtaagaac
    13381 atcattcagc cctttgcaca aagaaatggc ttcatcaagc agccaggcac cgttgggatc
    13441 aacggtaatc cgcgcatctg ggaagctttt cttcaatgca cgaacagtgt cgatttcttg
    13501 ctcgccaggt aacaccccgc ccttaagttt gaaatcttta aagccatagc gatcctgtga
    13561 ggcttccgcc agacgcacaa cggcttcgct gttcatcgct ttctgatggc gcaactgata
    13621 ccactcatgg ttgcccggcg tattttccag ataaggaaga tcggttttgg tccgatcacc
    13681 gatataaaac agataaccga ggacggtaat agtctcgcgt tgcttgcctg gccccaacag
    13741 ttcgcagacc ggaacattca gcgccttacc tagcaggtca agcaaggcgg cttccagcgc
    13801 cgccacggca ttaacgcgca attcaaaagt ccaggcacct ttgccgaagg tatcaaaatc
    13861 ggctgcctga ttacctttat gcacctgctg aaccacttta ttcaggcgcg caacttcctg
    13921 gcccagaacc atcggaatag catcgaccag tgtttgataa atcacctctc cgcccggcgc
    13981 ttcaccaatg ccggtatgcc cggcgttatc ggtgagtacc acaatattgc gggtgaaata
    14041 tgcgttatgt gccccaccaa tattaagcaa catgctatcg tgcccggcga ccggaatgac
    14101 tttcatatca gtaataacag gactggattg tgtcgtcatc ttattgcccc gcgacaggtt
    14161 tcagctcgat acgcttgata tcgcccacca gcaccaggta gctcagtacc gcgattaagg
    14221 catgaacacc aacataaatc agcgccccat taaaggagcc agtcgtgcca acgatataac
    14281 caattgcgat tggcgtgacg atgccagaaa tgttaccgaa catgttgaac aggccaccgg
    14341 aaagaccgct gatctctttt ggcgcggtat ctgccattac tgcccaaccc agcgccccga
    14401 tgcccttacc gaagaaggcc agcgccataa agccgatgat catccactca acgttgacgt
    14461 agttgcagaa caccatcacc atcgacagca acatgcccat tacgatcggt gttttacgcg
    14521 caatgttcag cgatcccgtg cggcgcatca gccagtcgga aataatccca cccagcacac
    14581 cgccgataaa accgcaaacc gccggaacgg aagccacaaa gcccgctttc agaatcgaca
    14641 tcccgcgtgc ctgcaccaga taaaccggga accaggtaat aaagaagtaa gtcagggcgt
    14701 tgatacagta ctgaccgata taaacgccga tcatcatccg tgacccgagc agctgtttga
    14761 tctgccccca cttcacgctg aacggcactt taactttagt gttttgctga tccatattga
    14821 tcagcgcgcc acccgcggcg atatactcca gctctttctg atttacgccc ggatgttgat
    14881 ttggctcgtg gatgactttc aaccagataa agctgatgac aatgcccaga ccgcccataa
    14941 agaagaagac gtgtgaccag cccacttcat gcgtcagcca gcccataatc ggcgcgaaga
    15001 taaccgttgc gaagtattgg gcggagttaa aaatcgacac cgccgttccc ctttcctgcg
    15061 ccggaaacca ggccgcaaca atgcgactgt tgccggggaa agatggcgct tcagcaagcc
    15121 caaccaggaa gcgcagcgta aacagggcaa cgataatgcc gaatccacta aagatatcga
    15181 cgaagccttg cagcaaggta aacatcgacc agataaagat cgaccagaag tagacgcgtt
    15241 ttgaaccaaa acggtccagc aaccagccac cagggatctg cccgataaca taagcccatg
    15301 agaaggcaga gaacacatag cccattccca cgggatcaag gccgatatct ttggccattt
    15361 ccgaaccggc aatagaaagc gtagcgcggt cgccgtagtt gaaggatgtg acgataaaca
    15421 acatcaccac tatccagtaa cgagcattcg tgcgtttttc cacactgctc gcagcctgac
    15481 ttaaagaact cattgttgca ctcctgaaaa ttcgcgttag ccacgctcac tctggactgc
    15541 gacatagcca ggaaatcaga ggtgacgtag ggtgtttttt gccgttttta taggtcgttc
    15601 gccgaatacg gcgcgtgttt atatctggca atagcagtat aaaaagcgcg ccatagcggc
    15661 tcaccgtgca acaacacaac attaatgcgt tcaatgaggc ccgattttgg cattagccct
    15721 ggacggtgga atccacttca cggaaatgaa aacaagaaca agaaaggaag ggttaaaacg
    15781 aagaaataaa gaagagtatg aaatggatcg cttgactcca ggcaaacgcc agtaaaaatc
    15841 cgcgctatga agcagttttt actggcattt gcctgaaaag attcgattca gcaccgctaa
    15901 aacgacattt accgctcgct gaacatatca ggacaacagc gtgccccact gttcgaccca
    15961 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gtatttcgcc
    16021 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcagaa
    16081 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16141 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16201 ggtcgtggac gtaaccacca gaacatactt atcctgataa ggcagccagt cgcttaattc
    16261 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16321 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16381 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16441 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16501 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16561 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16621 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    16681 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16741 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16801 actgctggct gcgagccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    16861 tccaccagtg gataatccag caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    16921 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    16981 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17041 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17101 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17161 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17221 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17281 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17341 tcagcaccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17401 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17461 cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacctcaat
    17521 aagaaaaact gccgcgaaga atagcaggaa taaaaaaagg gagcactgta ttcacagcgc
    17581 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17641 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17701 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17761 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    17821 cttcatccag aaacctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    17881 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    17941 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18001 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18061 gcttatttcc tggcgatctc ctacagagtc tgtaagagat ctctcacaac cagatggggt
    18121 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18181 gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18241 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18301 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18361 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18421 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18481 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18541 tagttgaaat aatgcacggt gaaggaacac cgtacagttc ctcacttaac ggccagcttt
    18601 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18661 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18721 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18781 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    18841 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    18901 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggttg
    18961 ccgtcggtca cgttgaactt gattacacca gcgcaaatct gattgagtcg aagagtttta
    19021 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19081 cgctggagcg cgacttaagc acttgcgccc agggtaaggt tagcgtggcg ttatatcgtc
    19141 ttgatgaact ggaaggccag ccgataggtc attttaatgg cacttgcatt gatgaccagg
    19201 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc acctgtggtg
    19261 aaaaagtagt ggaagagacg cttgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19321 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19381 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19441 aacgcatctt taatgacctg ttacgcttct gccagccaga aaaattgagc gtttacgcac
    19501 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19561 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19621 gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19681 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19741 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19801 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    19861 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    19921 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgctttgc
    19981 aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt ttacgggcaa atccataaca
    20041 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20101 ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc gccaaatatg gtggtctgct
    20161 ggggcggtca ctcaattaac gaaaatgagt atttgtatgc ccgtcgcgtc ggaaaccagc
    20221 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc gggagcgatg gaagcgccga
    20281 tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa agacagtcgt tttattggta
    20341 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    20401 tcatgccgga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20461 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20521 acccggccaa caaagatcag gttttaccat tgatcctcac cggcccgaaa gagagcgccg
    20581 actacttccg cgtactggac gagtttgtcg tacatacgct gggcgaaaac gcgcgccgcc
    20641 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20701 cgctggtgaa agaaaatcgc cgcgacactg gtgacgctta cagctttaac tggtcaatgc
    20761 gcattgcacc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    20821 agctttaccc ggatcaacct gttgaagtgc tggctgccga cctgcgccgt gcgttctccg
    20881 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    20941 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21001 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21061 cgtaatctcc gctcccccgg tgacgtttgt tgccgggttt tctcgttttt ggtcacttac
    21121 tcatcaactc atttcatttg ttatatgaat gtttcttacc accctcatgc gacaaatatc
    21181 atcacagtta atatgtcata caatttatat tgcaacgcaa acgtttccct attttcataa
    21241 accgtatttt atcgctatga aaaagaaatt atcgccatga ttaactaaaa gt
//
LOCUS       DBANUU010000081        20757 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000081 DBANUU010000000
VERSION     DBANUU010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.081"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(59..1708)
                     /gene="actP"
                     /locus_tag="ACIE68_002965"
     CDS             complement(59..1708)
                     /gene="actP"
                     /locus_tag="ACIE68_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFX5909246.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1705..2019)
                     /gene="yjcH"
                     /locus_tag="ACIE68_002966"
     CDS             complement(1705..2019)
                     /gene="yjcH"
                     /locus_tag="ACIE68_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFX5909247.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2220..4178)
                     /gene="acs"
                     /locus_tag="ACIE68_002967"
     CDS             complement(2220..4178)
                     /gene="acs"
                     /locus_tag="ACIE68_002967"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFX5909248.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4570..6006
                     /gene="nrfA"
                     /locus_tag="ACIE68_002968"
     CDS             4570..6006
                     /gene="nrfA"
                     /locus_tag="ACIE68_002968"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFX5909249.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPPKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6051..6617
                     /gene="nrfB"
                     /locus_tag="ACIE68_002969"
     CDS             6051..6617
                     /gene="nrfB"
                     /locus_tag="ACIE68_002969"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFX5909250.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6614..7285
                     /gene="nrfC"
                     /locus_tag="ACIE68_002970"
     CDS             6614..7285
                     /gene="nrfC"
                     /locus_tag="ACIE68_002970"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFX5909251.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7282..8238
                     /gene="nrfD"
                     /locus_tag="ACIE68_002971"
     CDS             7282..8238
                     /gene="nrfD"
                     /locus_tag="ACIE68_002971"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFX5909252.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPTVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8258..9976
                     /gene="nrfE"
                     /locus_tag="ACIE68_002972"
     CDS             8258..9976
                     /gene="nrfE"
                     /locus_tag="ACIE68_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFX5909253.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            9969..10352
                     /gene="nrfF"
                     /locus_tag="ACIE68_002973"
     CDS             9969..10352
                     /gene="nrfF"
                     /locus_tag="ACIE68_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFX5909254.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10349..10945
                     /gene="nrfG"
                     /locus_tag="ACIE68_002974"
     CDS             10349..10945
                     /gene="nrfG"
                     /locus_tag="ACIE68_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFX5909255.1"
                     /translation="MKQPKVPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11287..12600
                     /gene="gltP"
                     /locus_tag="ACIE68_002975"
     CDS             11287..12600
                     /gene="gltP"
                     /locus_tag="ACIE68_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFX5909256.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12678..13367)
                     /gene="yjcO"
                     /locus_tag="ACIE68_002976"
     CDS             complement(12678..13367)
                     /gene="yjcO"
                     /locus_tag="ACIE68_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFX5909257.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13461..15608)
                     /gene="fdhF"
                     /locus_tag="ACIE68_002977"
     CDS             complement(13461..15608)
                     /gene="fdhF"
                     /locus_tag="ACIE68_002977"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15189..15191),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFX5909258.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15806..17272)
                     /gene="mdtP"
                     /locus_tag="ACIE68_002978"
     CDS             complement(15806..17272)
                     /gene="mdtP"
                     /locus_tag="ACIE68_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFX5909259.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17269..19320)
                     /gene="mdtO"
                     /locus_tag="ACIE68_002979"
     CDS             complement(17269..19320)
                     /gene="mdtO"
                     /locus_tag="ACIE68_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFX5909260.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19320..20351)
                     /gene="mdtN"
                     /locus_tag="ACIE68_002980"
     CDS             complement(19320..20351)
                     /gene="mdtN"
                     /locus_tag="ACIE68_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFX5909261.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20370..20645)
                     /gene="ytcA"
                     /locus_tag="ACIE68_002981"
     CDS             complement(20370..20645)
                     /gene="ytcA"
                     /locus_tag="ACIE68_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFX5909262.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4676 a         5508 c         5691 g         4882 t
ORIGIN      
        1 tgatgcatgc tggagaggaa gagtattccc cggtcaggcg accggggaag gggagagatt
       61 aatgtgcgcg gccttgctca acgccaaagc cggtctggga gcggataaac tgtgcacgga
      121 acagttcacg ctcgcgcgca ccttccgctg agttatcggt tgccgagaag aaccagatgc
      181 cgaggaatgc cacgctgata gagaacagcg ccgggtattc atacgggaag atggcttttt
      241 cgtgaccaag gatctgtacc caaatcgtcg ggccgaggat catcagtacc actgcggtaa
      301 tcagccccag ccagccaccc atcatcgcgc cacgagtggt cagtttcgac cagtacatag
      361 aaagcagaat gatcgggaag ttacagctcg ctgcgatggc aaacgccagg cctaccatga
      421 aggcgatgtt ctgattctca aacagcacgc cgaggataat ggcaatcaca cctaaaatca
      481 gcacggtgat tttcgatacc cgcagttctt cacgttcggt cgcgcctttt ttgaagacgt
      541 tagcgtacag gtcatgcgaa accgctgatg cgcccgccag cgtcagacca gcaaccaccg
      601 caagaatggt ggcaaaagca accgctgaaa taaaaccgag gaacaggttg ccgcccactg
      661 cattcgccag gtgaacggcc gccatgttgt taccaccaat cagatggccc gccgcgtctt
      721 tatattccgg attcgcacca accagcatga tcgcgccgaa gccgataata aaggtcagaa
      781 tatagaagta gcccataaat ccggtagcgt agaacacgct cttacgcgct tcgcgggcat
      841 cgctgaccgt aaagaagcgc atcagaatgt gcggcaagcc agccgtacca aacatcagtc
      901 ccagacccag agagagcgcg gagatcggat ctttcaccag tccgcccggt ttcatgatgt
      961 cgacaccttt cgggtgtacc gccatcgctt cgctgaacag attgttgaag ctaaagccga
     1021 cgtgtttcat caccataaag gccataaagc tggcaccgaa cagcaacagc acggctttga
     1081 taatttgtac ccaggtggtc gccagcatgc cgccgaacag gacgtacatc atcatcagca
     1141 cgccgaccag caccaccgca atgtgatagt taaggccaaa cagcagctcg atcagtttac
     1201 ctgcacccac catctgggcg ataaggtaaa gcgccaccac caccagagaa ccacaggccg
     1261 aaagaatacg aatcggccct tgtttcagac ggtaagaggc cacatcggca aaggtgtagc
     1321 gccccaggtt acgcagacgt tcggcaatca ggaacagaat gatcggccag cccaccagga
     1381 agcccagcga gtaaattaag ccgtcatagc cggaggtaaa caccagcgcg gagatcccca
     1441 aaaatgaggc ggcggacata tagtcaccgg caatcgccag cccgttctgg aagccagtga
     1501 tattgccgcc tgcggtgtag tagtcgctac gagaacgtac gcgttttgat gcccagtagg
     1561 taatgccgag cgtaaacacg acgaaaatca ggaacataat aatcgcctgc cagttcgttg
     1621 gctggcgctc tacggccccg ctaatagcat ccgcggcgtt agctgcgaaa gggagtgtgg
     1681 cggcaagcgc cgtcagaact cttttcatga tgcttgtacc tcatgcagga cttcgttatt
     1741 aagacggtcg aattcgccgt tcgcccgcca gatgtagata ccggtgagaa caaaggagat
     1801 cacaatcact ccaacaccaa tcggaatacc gcgtgtgacg ctggtgttcg gattcagcgg
     1861 cgtgcccagc cagccgggcg cgaaggcgat cagtaaaata aagccgatat aaactgccag
     1921 cataataatc gacaggatgg tggcaaaccg ttgccgtttt tcgactaact ccctgaaatg
     1981 cgcattgtct tctatccgct gataaatagt gccattcatc acagattctc cagaggtaat
     2041 gtagggattg ttttaattcc cgctccctta tgggagaagg ttaacgctcg ggtaaccctt
     2101 gccgaatgta ggccggataa ggcgttatat gccgcatccg gcaatcaatg cctgatgcga
     2161 cgctgtcgcg tcttatcagg cctacaaacc gttaccgatt cgcatccggc aattgtgggt
     2221 tacgatggca tcgcgatagc ctgcttctct tcaagcagct tctcgacaac gccaggatcg
     2281 gcaagcgtcg aggtatcgcc caggttgctg gtatcgcccg ccgcaatttt gcgcagaata
     2341 cggcgcataa ttttgccgga acgggtttta ggcagggagt cggtccagtg cagcacgtct
     2401 ggcgtcgcca gcgggccaat ctctttacgc acccagttgc ggacttctgc gtacagttct
     2461 ggtgacggtt cctccccgtg attaagcgtg acgtaggcgt agatcgcctg acctttaata
     2521 ttgtgcggaa tacctactac ggcggcttcg gcaatcttcg gatgcgccac cagcgccgac
     2581 tcaatctctg ccgtccccag acggtgaccg gagacgttca gcacgtcgtc cacacgcccg
     2641 gtgatccagt aatagccatc ttcatcgcga cgcgcgccgt cgccgctgaa atacatattt
     2701 ttgaaggtgg agaagtaggt ctgttcaaaa cgttcgtgat cgccaaacag cgtacgcgcc
     2761 tgacccggcc aggagtcggt gatcaccagg ctgccttcgg tagcgccttc cagcgggtta
     2821 ccttcgttat cgaccagcgc cggttgcacg ccgaagaacg gacgtgttgc cgaaccggct
     2881 ttcagctcgg tagcgccagg cagcggcgtg atcatgaaac cgccagtttc ggtctgccac
     2941 caggtatcga ccaccggaca tttctcgttg ccgatttttt tccagtacca ctcccacgct
     3001 tctgggttaa ttggctcgcc cacggaaccg agaatgcgca gcgacgaacg gtcggtaccc
     3061 tcgatcgctt tatcgccttc cgccatcagc gcgcggatcg ccgtgggtgc ggtatagaga
     3121 atattgacct gatgcttgtc caccacctgc gccatacggg caggcgtcgg ccagttgggt
     3181 acgccttcaa acatcagcgt ggtcgcaccg caggccagcg ggccgtacag caagtaactg
     3241 tgtccggtca cccagcccac atcggcggtg caccagtaga tatcacccgg atgataatca
     3301 aagacatatt taaaggtcag cgccgcgtac accagataac cgccggtagt atgcagcaca
     3361 ccttttggct taccggtaga accggaggtg tagagaataa acagcggatc ttcggcgttc
     3421 atctcttccg cctggtgctg atcgctcgct tgctcaacca ggtcgtgcca ccacaggtcg
     3481 cgcccttcct gccagtcaat tttcccgcca gtacgcttca gtaccaccac atgctctacg
     3541 ctggtgacgt tcgggttttt cagcgcgtca tcaacgtttt tcttcagcgg aatactgcgc
     3601 ccggcacgca caccttcgtc ggaagtgatc accagtcgtg agttggaatc aataatgcgc
     3661 ccggcaacgg cttccggcga gaagccgccg aaaatcaccg aatgcaccgc gccaatgcgg
     3721 gcgcaggcca gcatcgcaac cgcggcttcc ggcaccatcg gcatataaat cgccaccaca
     3781 tcaccttttt taatgcccag ctcgagcagg gtattggcga agcggcagac gtcgcggtgc
     3841 agctctttat agctgatatg tttgctctgg ctggcgtcgt cgccttccca gatgatggcg
     3901 gtgcgatcgc cgttttcttg cagatggcgg tcaaggcagt ttgccgccag attcagcgtg
     3961 ccgtcctcgt accatttaat ggacacatta ccgggggcaa aggaggtatt tttcaccttc
     4021 tggtacggtt tgatccagtc gagaattttt ccctgttcgc cccagaaggt atcaggtacg
     4081 ttaatagatt gttgatacat cgcctcgtac tgctgagggt ttatcaggca acggtctgcg
     4141 atgttggcag gaatggtgtg tttgtgaatt tggctcatgc ttttgttctc cttgtaggat
     4201 gttaataata tgtggcataa gcgttaaatg taggggtatt ggcagttttg tttagtattt
     4261 gggcgacaga tcacgcaaaa gtagaattgt gcaaataaac ggcagggtaa tttttgaagg
     4321 tcaccaacaa aagttgatta attctttgag gaacatgcag ttatgcatgc tgttgaaaaa
     4381 gaggaagata ctgactaact ctaaagtggt attttacatg cacttacaat tgattaaaga
     4441 caacattcac agtgtggtta tttgttacac ataggggcga gcaatgtcat gacagtgtag
     4501 gtgcggttac tgtcgtgaaa acaataaaaa ccgccattgc aacaatggcg caattcggat
     4561 gaagccccta tgacaaggat aaaaataaac gcacgccgta tcttcagctt attgattcct
     4621 tttttctttt tcacttctgt tcacgctgaa caaacggcag ctcccccaaa acctgtaact
     4681 gtggaagcga agaatgaaac ctttgccccg cagcatcccg atcaatatct ctcctggaaa
     4741 gccacctcgg aacagtcaga gcgtgttgac gccctggcgg aagatccacg gctggtgatc
     4801 ctgtgggcgg ggtatccctt ctcgcgcgat tacaacaagc cgcgtggaca tgcctttgct
     4861 gtgaccgatg tgcgtgaaac cctgcgtacc ggtgcgccga aaaacgctga agatggtccg
     4921 ctaccgatgg cgtgctggag ttgtaaaagc ccggatgtgg cgcgtctgat ccagaaagac
     4981 ggcgaagatg gctacttcca cggtaagtgg gcgcgcggcg gcccggaaat cgtcaacaac
     5041 ttaggttgtg ccgactgcca taacaccgcc tcaccagagt tcgccaaagg caaaccggag
     5101 ttaacccttt cccgtccgta tgcggctcgc gcgatggaag ccattggtaa accttttgag
     5161 aaagccggac gtttcgacca gcaatcgatg gtttgcggtc agtgccatgt ggagtattac
     5221 ttcgacggca aaaacaaagc ggttaaattc ccgtgggatg acggcatgaa agtcgaaaat
     5281 atggagcagt attacgacaa aattgccttc tctgactgga ctaactccct gtcgaaaacg
     5341 ccaatgctga aagcgcagca cccggaatat gaaacctgga cagcgggcat tcacggtaaa
     5401 aacaacgtga cctgtatcga ctgccatatg ccaaaagtgc agaacgccga aggcaaactc
     5461 tacaccgacc ataaaattgg taatccgttt gataacttcg cccagacttg tgcgaactgc
     5521 catacccagg acaaagctgc cttgcaaaaa gtggtcgcgg aacgtaagca gtcgattaac
     5581 gacctgaaaa tcaaggttga agatcaactg gttcacgctc acttcgaagc gaaagcagcg
     5641 ctggatgcag gcgcgacgga agccgaaatg aagccaattc aggacgatat ccgtcatgcc
     5701 cagtggcgct gggatctggc gatcgcttcc cacggcattc atatgcacgc accggaagaa
     5761 ggtttacgga tgctcggtac ggcgatggat aaagcggcgg atgcacgcac caaactggcg
     5821 cgcctgctgg cgaccaaagg catcacccat gaaatccaga tcccggatat ctcaaccaaa
     5881 gagaaagccc agcaggccat tggcctgaac atggaacaaa tcaaggccga gaagcaggac
     5941 ttcatcaaaa cggtgatccc gcagtgggaa gaacaggcac gtaaaaacgg tctgttaagc
     6001 caataacccc gttccgcctc gcaaggggcg gaaaacacaa tggagtgaat atgagcgtat
     6061 tacgttcgtt attaactgcc ggggtgctgg cgtcgggcct gttgtggagc ctgaacggaa
     6121 ttaccgctac ccctgcggcg caggcatctg atgatcgcta cgaagttacc cagcagcgta
     6181 acccggatgc cgcctgtctg gactgtcata agccagatac cgaaggtatg catggaaaac
     6241 atgcttccgt tatcaacccg aataacaaac tgccggtcac ctgcaccaac tgtcacggcc
     6301 agccatcacc gcaacaccgc gaaggggtga aagatgtgat gcgctttaac gagccgatgt
     6361 acaaggttgg agagcagaac agcgtctgta tgtcctgtca tctgccggaa cagttgcaaa
     6421 aagcgttctg gccgcacgat gtccacgtaa ccaaagtggc gtgcgccagc tgccattccc
     6481 tgcatccaca gcaagatacg atgcagacgt taagtgacaa aggacggatt aagatttgcg
     6541 tcgattgcca cagcgatcag cgcaccaatc cgaactttaa cccggcgtct gttccgttgc
     6601 ttaaggagca gccatgacct ggtctcgtcg ccagtttctc accggcgtcg gcgtgctggc
     6661 agccgtcagc ggcaccgcag ggcgcgtggt ggcgaaaacg ttgaatatca atggggtgcg
     6721 ttacggcatg gtgcatgacg agtcgttatg catcggctgt accgcctgta tggatgcttg
     6781 tcgggaagtg aacaaggtgc cggaaggcgt ctcgcgcctg acgattattc gtagcgagcc
     6841 gcagggcgaa tttcctgatg tgaaatatcg cttcttccgt aagtcttgcc agcactgcga
     6901 tcatgcgccg tgcgttgacg tctgcccgac cggtgcgtct tttcgtgatg ccgctagcgg
     6961 gattgtcgat gttaatcctg atctctgcgt cggttgtcag tactgcatcg ccgcctgtcc
     7021 gtaccgcgtg cgctttatcc atccggtcac gaagacggcg gacaaatgcg atttctgccg
     7081 taaaaccaat ttgcaggccg gtaagctgcc cgcgtgcgtt gaggcttgcc cgacgaaggc
     7141 gctgacgttt ggcaatctgg acgatcctaa cagtgagatt tcgcaactgc tgcgccagaa
     7201 gcctacttac cgctacaagc tggcgctggg aaccaaaccg aagctgtacc gcgtaccgtt
     7261 taaatacggg gaggtgagcc aatgacgcag acttccgcat ttcattttga atcgctggtg
     7321 tgggactggc cgattgccat ctacctgttt ttgattggta tttctgccgg tctggtgacg
     7381 ctggccgtgc tgttacgtcg cttctacccg caggcgggcg gtgcagatag tacgttgctg
     7441 cgcaccacgc tgattgtcgg gccgggcgcg gtgatcctcg gtctgttgat cctcgtcttc
     7501 cacctgacaa gaccgtggac cttctggaag ctgatgttcc actacagttt tacctcggtg
     7561 atgtcgatgg gggtgatgct gtttcagctc tacatggtgg tgctggtgct gtggctggcg
     7621 aaaatctttg aacatgattt gcttgccctg caacaacgct ggttgccgaa gctggggatc
     7681 gtgcaaaagg ttctgagcct gctgacgccc gttcatcgcg gactggaaac attgatgctg
     7741 gtgttggcgg tgttgttggg ggcttatacc ggctttctgc tgtcggcgct gaaatcgtat
     7801 ccgttcctca ataacccgat cctgccggtg ctgttcctct tctccggcat ctcgtccggt
     7861 gcggcggtgg cgctgatcgc catggcgata cgccaacgca gtaacccgca ttccacggaa
     7921 gcgcagtttg tacaccgtat ggaaatcccc gtggtatggg gtgaaatctt cctgctggtg
     7981 gcgttttttg tcggtctggc gctgggcgat gacggtaaag tgcgtgcgct ggtggcggca
     8041 ttaggtggcg gtttctggac gtggtggttc tggcttggtg tcgccgggct ggggctgatt
     8101 gtgccaatgt tgctcaaacc gtgggtcaat cgcagttccg gcattcctac cgtgttggcg
     8161 gcgtgtgggg ccagtctggt cggcgtgttg atgctgcgct ttttcattct ctacgccggg
     8221 cagttaacgg tggcgtaagc cagaaaagag gtggtttctg gacgtattcc ttcctgaagt
     8281 cggttttctg gcgttgttgt taagtctcgg ggtcaacgtg ttgaccccgt tgacggcctt
     8341 tgcgggagtg cggttgcgct ggcctgccat gatgcgactc acttgcatcg gcattctggc
     8401 gcagttcgcg ctcctgctgc tcgcctttgg cgtactgacg tattgttttc tcatcagcga
     8461 tttctcggtc atttatgtcg cccaacatag ctacagcctg ctgtcgtggg aactcaaact
     8521 ggcggcggtg tggggcggcc atgaaggttc gctgctgctt tgggtgctgc tgctttccgc
     8581 ctggagcgcg ctgtttgcct ggcattatcg gcagcaaacc gatccgctat ttccgctgac
     8641 gctagccgtt ttatctctca tgctcgccgc actgctactg tttgtagtgc tgtggtccga
     8701 tcccttcgtg cggatatttc caccagcaat cgaaggccgc gatctcaatc cgatgctgca
     8761 acatcccggt cttatctttc atccaccgct gctttacctt ggctatggcg gtttgatggt
     8821 ggcggcgagc gtggcgctgg cgagtttact gcgcggcgag tttgatggtg cctgcgccag
     8881 aatttgttgg cgctgggcac tacctggctg gagcgcatta acggcgggga tcatcctcgg
     8941 ttcctggtgg gcctattgcg aactgggctg gggcggctgg tggttctggg atccggtgga
     9001 aaacgcctct ttattaccct ggctttctgc cactgcgctg ctgcacagtt tgtccctgac
     9061 acgccagcgg gagattttcc gccactggtc gctgttgctg gcgatagtaa ctctgatgct
     9121 gtcgctactg ggtaccttaa ttgtccgttc tggcattctg gtttcggttc atgcgttcgc
     9181 actggataac gtccgcgccg tgccgttgtt cagcctgttt gcactgatta gccttgcgtc
     9241 tctggctctg tatggctggc gagcgcggga cggtggtgcg gtggtgcgtt tttcggggtt
     9301 atcgcgggaa atgttaatcc tcgctacgct gttgctgttt tgcgcagtgc tactgatcgt
     9361 gctggtggga acgctttatc cgatgattta cggtctgctg ggctggggac gcctctccgt
     9421 tggcgcgccg tattttaacc gcgcgacgtt accgtttggc ctgttgatgc tggtggtgat
     9481 tgtgctggcg acgtttgtct ctggcaaacg cgtgcagctt ccggcgctgg tagctcatgc
     9541 gggcgtgctg ttatttgccg cggggatcgt ggtttccagc gtcagccgtc aggagatcag
     9601 cctgaattta cagccgggtc agcaggtgac gctggcagga tacaccttcc gttttgagcg
     9661 cctcgatctg caagccaaag gcaattacac cagcgaaaaa gcgatagtgg cactgtttga
     9721 ccatcagcaa cgcattggtg aactgatgcc ggagcggcgt ttttacgaag cacgtcgtca
     9781 gcaaatgatg gaaccgtcaa ttcgctggaa cggcatccat gactggtatg cggtcatggg
     9841 tgaaaaaacc ggagcggatc gttacgcttt tcgtttgtat gtacaaagcg gtgtgcgctg
     9901 gatctggggg ggaggattgt tgatgattgc gggcgcattg ttaagcggat ggcgggggaa
     9961 gaagcgcgat gaataaaggg cttctcacgc tactgctgtt atttacctgt tttgcgcgcg
    10021 ctcaggtcgt agacacctgg caattcgcca atccacaaca acagcaacag gcgttaaata
    10081 ttgccagcca gttacgttgt ccgcagtgcc agaatcaaaa cttactggaa tccaacgcgc
    10141 cggtggcagt cagtatgcgc catcaggttt acagcatggt ggcggagggg aagaacgaag
    10201 tcgaaatcat tggctggatg accgaacgct acggagattt tgttcgctat aacccaccgt
    10261 taacgggtca gacgctggtg ttatgggcgc tgccagtggt gttgttactg ctgatggcac
    10321 tgatcctctg gcgagtgagg gcgaagcgat gaaacagccc aaagtaccgg tgaaaatgct
    10381 gacaacactc acgatattga tggtatttct ctgtgtcggc agttatctgt taagcccaaa
    10441 atggcaggcg gtacgtgcgg aatatcagcg tcagcgcgat ccgctgcatc agtttgccag
    10501 ccagcaaacc ccggaagcgc agcttcaggc attgcaggat aaaatccgcg ctaatccaca
    10561 aaacagcgaa cagtgggcgt tactgggcga gtactatctg tggcaaaacg attacagcaa
    10621 ttcgctgctg gcgtaccgtc aggcgttgca actacgtggt gagaacgctg aactgtatgc
    10681 ggcgctggcg acggtgcttt attaccaggc cagccagcat atgaccgccc agactcgcgc
    10741 aatgatcgat aaagccctcg cgctggactc taacgaaatc accgctctga tgctgctggc
    10801 ttccgatgcg tttatgcagg cgaattacgc gcaagccatc gaactatggc aaaaagtgat
    10861 ggatctcaac tcaccccgga tcaaccggac acagctggtc gagtcgatta atatggcgaa
    10921 attgttgcag cggagatcag attaatcatc tggtgatgaa gattatgtta ttgagctgta
    10981 taaaaaataa tcgaatgaac atatgccaaa aataatcacc aatcagtatt attgcagatt
    11041 aaacaaataa aaatctttcc ataacaaatg gttattcatt aatcctgcca taaactcgcg
    11101 ttattatgca ttaatgcagc gaaaagctct gttgttaaag ggttgcgcaa cataccgcgc
    11161 aaatgatact gatcataagc gttaaaaaaa tctacaaacc aacgcaacac aattcatgcc
    11221 ctggcagtat gtcacgttct cgcgtttctg aacggggaac ggcgctccat tgaggaagtt
    11281 attctgatga aaagtataaa attcagcctg gcctggcaga ttctgtttgc tatggtgctg
    11341 ggcattctcc tgggaagcta cctgcactac catagcgaca gccgcgactg gctggtcgtc
    11401 aatttgctct ctccggcggg tgatatcttc atccatctga tcaaaatgat tgttgtgccg
    11461 attgtgatct ccacgctggt ggtgggtatc gcgggtgttg gtgatgccaa acagctcggg
    11521 cgtattggcg cgaaaaccat tatttacttc gaggtgatca ccaccgtcgc catcattttg
    11581 gggatcactc tggcgaacgt cttccagccc ggtgccgggg tggatatgtc gcagctggcg
    11641 accgtcgata tctcgaaata tcagagcact acggaagcgg tacaaagcag ttcccacggc
    11701 attatgggca cgattttgtc gctggtgccg acgaacattg tggcgtcgat ggcgaaaggc
    11761 gaaatgctgc cgatcatctt cttctcggtg ctgtttggtc tggggctttc ctccctgccc
    11821 gcgacgcatc gtgaaccgct ggtgaccgtg ttccgctcca tctctgaaac catgtttaaa
    11881 gtgactcaca tggtgatgcg ttatgcaccg gtgggtgtgt ttgcgctgat tgcggtgacg
    11941 gtggctaact ttggtttctc ttctctgtgg ccactggcga aactggtgct gctggtgcat
    12001 ttcgccattc tgttcttcgc gctggtagtg ctgggaattg tggcgcgcct gtgcgggtta
    12061 agcgtctgga tcctgattcg tattctgaaa gatgagctga ttctggcgta ctccactgcc
    12121 agctctgaaa gcgtactgcc gcgaattatt gagaagatgg aagcctacgg agcaccggcg
    12181 tcgatcacca gcttcgtggt gccgaccggt tactctttta accttgatgg ttcgacgctg
    12241 tatcaaagta ttgccgctat tttcatcgct cagctgtatg gcattgacct gtccatctgg
    12301 caggaaatca ttctggtgct gacgctgatg gtgacctcga aagggattgc tggcgtgccg
    12361 ggcgtgtcgt ttgtggtgct gctggcaacg ctgggtagcg taggtattcc gctggaaggc
    12421 ctggcgttta ttgctggtgt tgaccgtatc ctcgacatgg cgcgtactgc gctgaacgtg
    12481 gtgggtaatg cgctggcggt gctggtgatt gccaagtggg aacacaaatt tgaccgcaaa
    12541 aaggcgctgg cttatgagcg tgaagtgctg ggcaaatttg ataaaactgc ggatcaataa
    12601 ttgaagattg ccggggttat ccacccggca atgtgtggat gcctgatgcg acgctggtgc
    12661 gtcttatcag gcctacctta accgttggtt aatttttcaa actcttcaca cccggtatca
    12721 aacccttcca tacagctcag gttcaaccag tgcagcgcct tttgcttgtt cttctcgata
    12781 aaaccttctt caccgtttaa gaacatcatt cccgcccagt actcggaata accggttcgg
    12841 gaaattgcag agctgcgttt gaaataccag gttgccttgt catcgtcggc cttaatgccc
    12901 acgccgttgg cgtaaatcaa accaagcagc atttgggcat cgacggcaga gtcgttctcc
    12961 agatcttccg aggcgttttc taacagcgaa atcgcctttg gataatccgg tttacccgct
    13021 tgagtattta ccagaatatg cgccagcgtt acttcacctt ctttactgcc cgcttgcgcc
    13081 gctttctctg caagaacttt tgcttgtgga tagtccagac tgaccggatt ggtgattttg
    13141 atctgcgcca gcagcgcgca ggcatcagca tcaccgctgt cggcggcttt ctgtgcccaa
    13201 tattcggctt tgctcaaatc gccggagcta aaccagctgt cggcaagaaa atattgtgcg
    13261 cgtcggtccc cggcctctgc tgcctttaaa tactggctgc caggctcgga gtcgttcgcg
    13321 tgggcaaaga atgtcaaaaa caacatcaat gcgataattt ttttcatttt gataatacga
    13381 ttgggtaggg agtaaccagt ataaagagtt cgaatggata aaaaaacagc ctccgaaagg
    13441 aggctgtaga aaggactgta ttacgccagt gccgcttcgc gcaggcgagt tttcaacttg
    13501 ttgtactcgt caatcacgta ctgctcggcg gcgcgctgat cggcgatcgg ctcgacgcgc
    13561 acggcgcagt atttgtattc cggcgttttc gtaatcgggc ttaagttttc ggtaaccagc
    13621 tcgttacagg caccaatcca ccactggtag gtcatgtaaa tcgccccttt gttcggacga
    13681 tcgctgacct gcgcacgggt gataattttg cctttacgcg agtgcaccca aaccaatgcc
    13741 tcatcttcaa tacccagacg tttggcgtct tcggtattga tttgtgcgta gccaggttca
    13801 tcagccagcg ccgccagtgc cgcacagttg ccggtcatcg aacggcaaga gtagtgacca
    13861 acttcacgca ccgttgacag taccatcggg tactcgtcgg tgagtttgtc gattggcgct
    13921 acccagtcgc aggtgaagaa ctgtgccaga ccgttcgggg tatcaaactt ctctttaaac
    13981 agataagaag tcccctgatc ggcatctgaa gtatcgcggc aaggccactg aatgaagccc
    14041 agttcgccca ttttctcgta agtcgcaccg tagaaatccg ggcacagatg acgcaactca
    14101 tcccagatct cctgggtgtt gttgtagtgc atcggataac ccatacgggt ggcgatttca
    14161 ctgatgattt gccagtccgt tttcagatcc catttcggtt caactgcttt gaagaaacgc
    14221 tggaagccac ggtcagccgc agtaaacacg ccttcatgct cgccccacga cgttgacggc
    14281 aaaatgacat ccgccgccga cgcggttttg gtcataaaga tgtcctgaac gataaccagt
    14341 tccagatctt caaaggcttt acgtactgcc gacagctccg cgtcagtttg cagcggatct
    14401 tcgcccataa tgtacgcggc acgcacttcg ccatgcgctg cgcggtgcgg cagctcgctg
    14461 atgcgataac cggtatgagc aggcaggctt tccacgcccc aggctttggc gaatttctcg
    14521 cggttagccg gatctttcac gtactggtat cccggatacg tatccggcag cgcgcccata
    14581 tcgcaggcac cctgcacgtt gttctgacca cgaaccgggt ttacacccgc atgcggctta
    14641 ccgaggttac cggtcagcat cgcgaggctg gtcagagaac gcacggtttc cacgccctgg
    14701 tagaactggg ttacacccat gccccacagg atggcggcgc ttttcgcctg ggcatacatc
    14761 cgtgctgcct gacgaatctc actggcgctg acgccggtga tatcttcaac cgactccggc
    14821 gtgtagcctt caacgatttt acgatactct tcaaagcctt ctgtacggga agcgacgaac
    14881 gctttgtcgt acagattttc ttcaataatg acatggccca tcgcattcaa cagcgcgatg
    14941 ttcgagccgt ttttcagtgc aatgtgcatg tcagcaatgc gcgcggtttc aattttgcgc
    15001 ggatcgcaga cgataatttt cgccccgtta cgtttagcgt taattacgtg attcgccacg
    15061 attgggtggg aatccgccgg gttgtacccg aaaacgaaca ctaaatcggt attatcaatt
    15121 tcgttaatag cattgctcat tgcgccatta ccgaccgatt ggtgcagacc tgcaaccgat
    15181 gggccgtgtc agacacgagc gcagcagtca acgttattgg taccaataac ggcgcgcgca
    15241 aatttttgca ttacatagtt ggtttcgtta cccgtaccac gcgaggagcc ggtcgtctgg
    15301 atggcatccg gaccgtactt ctctttgatg gcgctcaggc gctcggcaac gtaattcagt
    15361 gcctcatccc aggaaacagg ttcgagtttg ccgccacgct ggcgacggat catgggggtt
    15421 ttcaggcgcg gggtcaggat ctgggtatcg ttaatgaaat cccagccata ataacccttc
    15481 agacacaggg taccctggtt ggttttcccc tgcgctgcct ccgcccggac gattttgccg
    15541 ttatcgacga ccaggttgat tttgcaacct gatgcgcaat aggggcaaac cgtgacgact
    15601 tttttcatcg gtctcgctcc agttaatcaa atcacgcata cgcgctctcg actacagtat
    15661 gcatctttta tgccacattt tatttggggt cattccctga tattacgggc actatttatt
    15721 caaaactctg acgaaaaaca ggctgtcgtc agttttgacg tgacgaaacg aaataccgcg
    15781 tgacagccat cacgcggcag acattttatt ttttctcgac gacgggagcc gcctgatacc
    15841 cgccgcccag cgatttcatc aactgaatgc tttggatcac ccggcggctg tccagcatca
    15901 gtaatgacat ctcttcggca agtactggca accgggcttc agtggcctgt aagcggctgg
    15961 ttaagccgcg ctgataggcg gcctcggcag cgcgctgggt gaagcgcgtt gcttccacgc
    16021 gttcagcctg catttctcgc tcgtcgttga gcgtttgcag acgcgtgccg ttgacggcaa
    16081 cgtcacgcac cgcgttcagt actgactggt tgtaacgttc aatcatcatg ttgctggcgg
    16141 cgcgcgtgcc ttcgagattg gcattcaacc gtccaccgtc aaacagcggc aatttcagac
    16201 ccgggatgaa gttgaactgg cgactggttt ttttgaataa ggtatccagg tggatggcat
    16261 caaggccgaa aaacgctttg atatcaaagc tcgggtagaa cagcgcccgc gcggaatcca
    16321 cctgatctaa tgacgcctga acataccagc gcatggcttg cagatccggg cgtctggcga
    16381 gcaactcata agagagtgtc gccggaatgc cggtctggac tcgtggtaat gccaccggtt
    16441 tgatctccgg catatcgctg gcccccgcgc caatcaatgc acgcagagat tctcgcgttt
    16501 cggtgatttg ccctttgacg gcagcaattt gtttatcgac cgccagaatc tgtgcccgcg
    16561 cgccgtggaa aggcacttgt gcttccagac cgtgcgccac tttactctgg tgcgctttca
    16621 ccgcgtaatc aatcacatcg cgagtttgtt ctaacagatc gagcatctga tagctggcct
    16681 gcatactgta ataaagctgc gctacgcccg tggtcagcga tagctctact gctgcggttt
    16741 ctgccagcgc ggcattatgc gcgccaatgg cggcggcaac cgctgagcga tgcacacccc
    16801 acaaatcaag atccagtccg gcaaacaaac ctactgtggc ttccgtatag tacggcccgt
    16861 ccatacccag tgcgggcgca tccatcgcat aagggcttaa aaagccgttc gccgagacgc
    16921 gttggcggtt gagcatccct aacgctgcga cctgtaattg tgaaccggca tctaacaaat
    16981 cggcctggga ctgcgctttt tcttcccgca gtttcgcttc ggcgagggtg tgtgaaccac
    17041 ttagcgtccg ttggatcagc gcatccagct gcgggtcatt gagttgtttc caccactgcg
    17101 cctgcggcca gccggagctg gcaagatgaa tatcgtcggc cagtttgatt tgttccggtt
    17161 tgagctgttg atgaggtgca gaatccttac gtaccagggc acagccggaa atcagcgtcg
    17221 tgctgccgag aatgctgcac aacagcagac gtgaaagttg acgattgatc attgcgtggc
    17281 tccttgtgcc tgttccgtgg cgggcgttaa tgccggggct gtccagtctg gcaagcgggc
    17341 gaaaagctgg cagacgtgtt gctcctgttt taataaggtg ggcaggatgg cctgcgaggc
    17401 gggtgtctct tcaagcgtta tttgaggaga acggctgagt gcggttgcca gaccggcagc
    17461 gtatttctcc agggcgtcgg caaactgagc tgctctgtcc ggctgtagtg cgttatccag
    17521 cgcctgcgcc gagttccagg tggcatccca ggcgtgaaga atatcgcggc cctgacgaat
    17581 aaccgtttgc gaacgttcaa tcagtaaggc gcgttcttcg ctgtccagtt gacgctccag
    17641 cgccacgcgt tggcacatct cttcacaggc attaaacgcc gcatgcagac cgatacgaat
    17701 ttgcagataa gtgcgcagag ccgtgacttc ctgctggcgt gggatccgca ttactttact
    17761 taacataccc agcgtgccag ccagtttttg cggcagtgtg cgcgcttcac tttcaggcca
    17821 gacaaaggtg taaatcaccg cggacaccac ggtaccaatg atgataccca tggcgcgatc
    17881 gcgaatttcc accaggtcgt acaccgggcc aaaaacgttt tcgagcgtgg cgagcgcgaa
    17941 ggtgaccacc atctgtgtgc cgatataaga agagcgttca gagctggtgg caatccatgc
    18001 gcccaacagg aaaatcggtg ccagcacaaa cagcaattcg acaatattgt ccagccaggg
    18061 catgaccagt agcgtgaata acagcgccag aatcgcgccg caaaaggccc cgccaaaacg
    18121 cagcaccatc ttctggtacg acgaaccgac atttgggtta gcgacgatca cgcatgtcag
    18181 catacaggtg tgaatgcctt cccagtccac gccgctgtag aaggtgtaac agatcaaaca
    18241 ggcgagcagt gtttttaccg cgtagcgcat atagtctgga ttggtaaaag catcggcgac
    18301 cattgatggc ggtttggcgg cgggcgttgg cggcgtattc gggtccatct gacccagttg
    18361 taacaacgtc tggcagatat tctccaggtt acattcccgt gccgccatcg cttcactttc
    18421 agtgatccgc cagtcgcttt gccagcactg accttcagca acggcatgct gcagcttatt
    18481 gatttctgag gctaattttt gccgaaattc aataattgcc tgagaatcag caaaagaggt
    18541 gggatcgtag cgattcagcg tcgagtaaat gtaggttacc gttgccacgc agctttgcca
    18601 ccatgcgctt tgagttcgcc agttggcatc gtccgcgaga caaaagacat tcagtttttg
    18661 cagcgccagc gcctctcttt caatccgcgt ttcgggtagc ggtgcgaggc tgtccgtcag
    18721 gtgactaatg gcatcatcaa gccgatcatt aagcgcttga tgcatttgcg taatggcacg
    18781 actgggaaac cacagcacgc cgattaacgt catcagcaag gttggataga ggccaacaac
    18841 gatacaccac agcgttaagc gcacgaccac ttccggatag tcgagcatgg cggggaaggt
    18901 ttgcccgtaa atggcgacaa tggcgacagc gaaaaagacc agtcccagcc gatgggtgcg
    18961 catcaaaaac atacagccca tcaggatcgg cccggcgatg atcaatcgga tcaacggttc
    19021 gccgtatgac catttgtaga tcaaaaacag gctgccgatc tccagcaccg tggcaaccac
    19081 aaacaagatc gcgacaaatt tggtgtaaaa cgcgttcgac tgaataccgt aaaacagcac
    19141 tgccagcgat aacgccacaa aagggatctc aaaagtcatc gagatcagaa tcaccagcag
    19201 gcagcctacc cagagttgca cgatctgcgg cacgcggccg ggtcgccgct cgcttaactc
    19261 ttcatgaaag aacgccagca gcctgaccac cggtaatggc agggagttga gcgcgctcat
    19321 tattgcggct caagattagc gactgccgaa gcgccgatgc ggaacatttc cgggtcaggt
    19381 ttatcgacca tgattttgac cggaaaacgc tgggcaacgc ggacccagtt aatagagcga
    19441 gaaactttcg gcaggccgcc cagcaccagg ccgccgtcat ccggtagcac gccgtagcca
    19501 atcgaatcca ctttaccctc gaaggttttg ccgctgtcac tcatcaggcg aatcgttgcg
    19561 ggtgtacctg agcgaatatt tttcagatcg gtttcgcgga agtttgcgat cacgtaccag
    19621 tgacgagtgt cgattagggt aaaaataggg cgcatggcag aagcaaattg cccgacggag
    19681 gttttgaggg aaatgacccg gccatcaaac ggcgcgcgaa cggtcgccat ttccagatga
    19741 agtttggtca gggcaatatc cgcttcgacc gccgcacgtt gcgccaccag cgcatcgacg
    19801 ccgctgacgg cgctggcggc tgactgcgcc tgtaacaata cggcattaag atcggcttct
    19861 gcggcgcgct gcgcagttct tgcacggtca acgtcttccg ctgagacaaa accctctttc
    19921 agtaatggct cggtgcggcg taatgtatct gtggcctgtt tcgcggcggc acgggctttt
    19981 tctaccgtgg cattaatcga gtcggcacca aactgttgcg catcaacgct acgctgggtg
    20041 agcataattt gcttatccag cgccgcgagg gaggcttcag ctttcgccag attggcttcg
    20101 tacgggcgcg ggtcaatgcg gaacagcaaa tcgccctgtt tgactgcctg gttgtcggtg
    20161 accgccagtt ctacaatgcg gccgctgact tccggcacca catcaatggt atctgctgac
    20221 gcgtaagcgt cattagttga tggcgcactg tctacgcgcc agataacgaa aacaagggca
    20281 accagcgcca atgcaaccac taacagagca gggaatttac tgcgaggagc ttttttcggc
    20341 gtactttcca tgagcgcgtc tcaaatagat tagaaaaatg ccagccagaa cagcatggcg
    20401 tagagagcaa aaagggcggt ataaataatt ccgacaaatg ccagattgat atttgcccgc
    20461 tgaataactc gcctcgtgat gagcatcaaa ataaggctgg caatggcgca gaaaaaccag
    20521 ctgggataat aagcaccgat caccgggatt gccggagata atgagcatcc cgaaaccatg
    20581 aagacgggaa ttatccaggc tgtttttttg agttgcatcg ccatgcgaga gagaactgtt
    20641 ggcatattaa atacatcctg tctgctgcga acaatatctg tagtgaatat caatatccat
    20701 tgaaggcgtc agtatggcta ttttctcgat gaataaaaat agctttattc agccacc
//
LOCUS       DBANUU010000082        20207 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000082 DBANUU010000000
VERSION     DBANUU010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.082"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(142..888)
                     /gene="cutC"
                     /locus_tag="ACIE68_002982"
     CDS             complement(142..888)
                     /gene="cutC"
                     /locus_tag="ACIE68_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFX5909263.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(902..1468)
                     /gene="yecM"
                     /locus_tag="ACIE68_002983"
     CDS             complement(902..1468)
                     /gene="yecM"
                     /locus_tag="ACIE68_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFX5909264.1"
                     /translation="MANWQSIDELQDIASDLPRFIHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1684..3417
                     /gene="argS"
                     /locus_tag="ACIE68_002984"
     CDS             1684..3417
                     /gene="argS"
                     /locus_tag="ACIE68_002984"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFX5909265.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3459..3503)
                     /gene="yecV"
                     /locus_tag="ACIE68_002985"
     CDS             complement(3459..3503)
                     /gene="yecV"
                     /locus_tag="ACIE68_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFX5909266.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3594..4082
                     /gene="yecT"
                     /locus_tag="ACIE68_002986"
     CDS             3594..4082
                     /gene="yecT"
                     /locus_tag="ACIE68_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFX5909267.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DVNVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4202..4594)
                     /gene="flhe"
                     /locus_tag="ACIE68_002987"
     CDS             complement(4202..4594)
                     /gene="flhe"
                     /locus_tag="ACIE68_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFX5909268.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4594..6672)
                     /gene="flhA"
                     /locus_tag="ACIE68_002988"
     CDS             complement(4594..6672)
                     /gene="flhA"
                     /locus_tag="ACIE68_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFX5909269.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NIVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6665..7813)
                     /gene="flhB"
                     /locus_tag="ACIE68_002989"
     CDS             complement(6665..7813)
                     /gene="flhB"
                     /locus_tag="ACIE68_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFX5909270.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8015..8659)
                     /gene="cheZ"
                     /locus_tag="ACIE68_002990"
     CDS             complement(8015..8659)
                     /gene="cheZ"
                     /locus_tag="ACIE68_002990"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFX5909271.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8670..9059)
                     /gene="cheY"
                     /locus_tag="ACIE68_002991"
     CDS             complement(8670..9059)
                     /gene="cheY"
                     /locus_tag="ACIE68_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFX5909272.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9074..10123)
                     /gene="cheB"
                     /locus_tag="ACIE68_002992"
     CDS             complement(9074..10123)
                     /gene="cheB"
                     /locus_tag="ACIE68_002992"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFX5909273.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYNEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10126..10986)
                     /gene="cheR"
                     /locus_tag="ACIE68_002993"
     CDS             complement(10126..10986)
                     /gene="cheR"
                     /locus_tag="ACIE68_002993"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFX5909274.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11005..12606)
                     /gene="tap"
                     /locus_tag="ACIE68_002994"
     CDS             complement(11005..12606)
                     /gene="tap"
                     /locus_tag="ACIE68_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFX5909275.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRGLQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESVQLQIAPVVS"
     gene            complement(12652..14313)
                     /gene="tar"
                     /locus_tag="ACIE68_002995"
     CDS             complement(12652..14313)
                     /gene="tar"
                     /locus_tag="ACIE68_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFX5909276.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14458..14961)
                     /gene="cheW"
                     /locus_tag="ACIE68_002996"
     CDS             complement(14458..14961)
                     /gene="cheW"
                     /locus_tag="ACIE68_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFX5909277.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SASSEVA"
     gene            complement(14982..16946)
                     /gene="cheA"
                     /locus_tag="ACIE68_002997"
     CDS             complement(14982..16946)
                     /gene="cheA"
                     /locus_tag="ACIE68_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFX5909278.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16951..17877)
                     /gene="motB"
                     /locus_tag="ACIE68_002998"
     CDS             complement(16951..17877)
                     /gene="motB"
                     /locus_tag="ACIE68_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFX5909279.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17874..18761)
                     /gene="motA"
                     /locus_tag="ACIE68_002999"
     CDS             complement(17874..18761)
                     /gene="motA"
                     /locus_tag="ACIE68_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFX5909280.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLATVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18888..19466)
                     /gene="flhC"
                     /locus_tag="ACIE68_003000"
     CDS             complement(18888..19466)
                     /gene="flhC"
                     /locus_tag="ACIE68_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFX5909281.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19469..19819)
                     /gene="flhD"
                     /locus_tag="ACIE68_003001"
     CDS             complement(19469..19819)
                     /gene="flhD"
                     /locus_tag="ACIE68_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFX5909282.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4819 a         5565 c         4945 g         4878 t
ORIGIN      
        1 tgcgcactgt cacccgctca acagatcacg gttatcgttc gttttttata ctgttcaggg
       61 ataaaaaaag gcccctgttg aaattgcagg ggcctggtac gagcaagcat catattgggc
      121 gacatgatgc aacggtaaaa atcatttggc ctgatggcgt tcaatgattc ctttcatttc
      181 agcaaccgcc gccccgtcta cgatataacg cgaatactcg tccgcgtgtt catctgatga
      241 catggacaat ccttgattac gataacgcat cggtgaggct tgccacgctc ccgcggagct
      301 atggacttcc agcactccgg catcgaggaa gtggtgcagg ttttctgcac ggactcctgc
      361 tccggccata atgattggag catcacgatg ggcaataagt tccataattt ttgataaacc
      421 ttgcagtgcg tctgattttt gccctgatgt cagtacccgg gcaatgccca attccgcaag
      481 attattgagt gtatataaag ggttagcgca catatcgaag gcgcgatgaa aagtcactgc
      541 cagcggaccg gcagcagcca ttattttttc cattcgtggc atatcgacat tcccgtcaac
      601 atcgagaacg cccgtcacca gtccaggaaa acctaattcg cggaccgtgc gcacatcctc
      661 aagaatggcg gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg ggcgaattat
      721 cggatgcaca gggatcgtca cccgctggcg cacggatttc agtacaccca acgacggcgt
      781 taagcccccc tcttttgggg ctgcgcataa ttcaactctg tctgcgccgt tttgctgcgc
      841 cgttagtgca cattccatgc tgtaacagca aatttccagt aatgccattt ttactcctta
      901 attacgccga ctgctcgctg gcaacgatct cttcaatgga ccacggatga aacttaatag
      961 ttgttttgcc atcggtgacc gccagcgtcg gattcggtaa tcgctcatgc tcaccttttg
     1021 gcgaactggt tttcactgag atccccggta ggctgagtcc ttcatcagaa agcaatgcca
     1081 gcgcacgggc attcagcgtt tctggatcac ccggcagaac gatttcaata tgttcccaac
     1141 cttcgtgtgg gtaacgtttt tccccgggcc acggtagctc cacaatagaa aactgccagt
     1201 gcgcaacctg taccggttca tgcaatttaa acagacaaat cggtctgcca ttgatcatat
     1261 tttctgacaa aagctcgcca cactgttcaa acccgcgacg ccagcgttca gcagtggcgt
     1321 tttgatggca acgcaaagaa atgtgatcgg cagtcagcgg agtgatattc aaccccagac
     1381 ggcgggaaag ttcatctaat gcgtggataa atcgcggtaa atccgatgca atatcctgca
     1441 gctcgtcgat agattgccag ttcgccataa tcactcttcg tctttcagta aaagcgttaa
     1501 tttaccctgt tgccctgtgc caaccaaccg ctgatttcac gccgcttctg atgcaatagt
     1561 gaaaacggca atacgccacg cgcacgttgc tgacgaaaac agccatttgc agtatactcc
     1621 cgccctaatt tctttaactg gtgcgggcaa tttttgctcg cttcatcaat gtaaggtatt
     1681 ccggtgaata ttcaggctct tctctcagaa aaagtccgtc aggccatgat tgcggcaggc
     1741 gcgcctgcgg attgcgaacc gcaggttcgt cagtcagcaa aagttcagtt cggcgactat
     1801 caggctaacg gcatgatggc agttgctaaa aaactgggta tggcaccgcg acaattagca
     1861 gagcaggtgc tgactcatct ggatcttaac ggtatcgcca gcaaagttga gatcgccggt
     1921 ccaggcttta tcaacatttt ccttgatccg gcattcctgg ctgaacatgt tcagcaggcg
     1981 ctggcgtccg atcgtctcgg tgttgctacg ccagaaaaac agaccattgt ggttgactac
     2041 tctgcgccaa acgtggcgaa agagatgcat gtcggtcacc tgcgctctac cattattggt
     2101 gacgcagcag tgcgtactct ggagttcctc ggtcacaaag tgattcgcgc aaaccacgtc
     2161 ggcgactggg gcactcagtt cggtatgctg attgcatggc tggaaaagca gcagcaggaa
     2221 aacgccggtg aaatggagct ggctgacctt gaaggtttct accgcgatgc gaaaaagcat
     2281 tacgatgaag atgaagagtt cgccgagcgc gcacgtaact acgtggtaaa actgcaaagc
     2341 ggtgacgaat atttccgcga gatgtggcgc aaactggtcg acatcaccat gacgcagaac
     2401 cagatcacct acgatcgtct caacgtgacg ctgacccgtg atgacgtgat gggcgaaagc
     2461 ctctacaacc cgatgctgcc aggaattgtg gcggatctca aagccaaagg tctggcagta
     2521 gaaagcgaag gggcgaccgt cgtattcctt gatgagttta aaaacaagga aggcgaaccg
     2581 atgggcgtga tcattcagaa gaaagatggc ggctatctct acaccaccac tgatatcgcc
     2641 tgtgcgaaat atcgttatga aacactgcat gccgatcgcg tgctgtatta catcgactcc
     2701 cgtcagcatc aacacctgat gcaggcatgg gcgatcgtcc gtaaagcagg ctatgtaccg
     2761 gaatccgtac cgctggaaca ccacatgttc ggcatgatgc tgggtaaaga cggcaaaccg
     2821 ttcaaaaccc gcgcgggtgg tacagtgaaa ctggccgatc tgctggatga agccctggaa
     2881 cgtgcacgcc gtctggtggc agaaaagaac ccggatatgc cagccgacga gctggaaaaa
     2941 ctggctaacg cggttggtat tggtgcggtg aaatatgcgg atctctccaa aaaccgcacc
     3001 acggactaca tcttcgactg ggacaacatg ctggcgtttg agggtaatac cgcgccatac
     3061 atgcagtatg catacacgcg tgtattgtcc gtgttccgta aagcagaaat tgacgaagag
     3121 caactggctg cagctccggt tatcatccgt gaagatcgtg aagcgcaact ggcagctcgc
     3181 ctgctgcagt ttgaagaaac cctcaccgtg gttgcccgtg aaggcacgcc gcatgtaatg
     3241 tgtgcttacc tgtacgatct ggccggtctg ttctctggct tctacgagca ctgcccgatc
     3301 ctcagcgcag aaaacgaaga agtgcgtaac agccgtctaa aactggcaca actgacggcg
     3361 aagacgctga agctgggtct ggatacgctg ggtattgaga ctgtagagcg tatgtaatcg
     3421 atttttcgtg agagtgaagc ctgatcaggg ttagccgatc aggctttttt attgccatct
     3481 aaatgtattc tgaaaatgga catgccattg ttttctcact gttggataag aggccagaag
     3541 cgtaatatcc ggccccaggg aaacgataac ggttgaattt aaggaatacc gcagtgttta
     3601 aatttcttgt attaacatta ggcattatct cttgccaggc ttacgcagaa gatacggtta
     3661 tagtaaacga ccatgacatt tcagccatca aagattgttg gcaaaaaaat tcagatgatg
     3721 atactgacgt taacgtgatc aaatcatgcc tgcgacaaga atacaatctc gtcgatgcgc
     3781 aattaaataa agcctatggt gaagcttatc gttatataga acaagtgcca cgcacaggtg
     3841 taaaaaaacc tgataccgaa caacttaact tgcttaaaaa atcacagcga gcctggctgg
     3901 attttaggga caaagaatgt gaattaatcc tttcaaatga ggacgttcag gatttaagtg
     3961 acccttattc tgaatcagaa tggctctcat gtatgatcat acagaccaat acgcgaactc
     4021 gccagttgca gctataccgt aactctgaag atttttatcc aagccctttg acaagaggat
     4081 aattcacatc tttttggcat gttttgttgc aagctattcc tgataaataa ttgcaacaag
     4141 acatcgagcc tttttcactg agttattaaa catactcgcg agcgcgtaat ttttttgtcc
     4201 ttcagcgata attcacaatc acttcattac gttgtacctt tagcggtgga attaaccgcc
     4261 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
     4321 ccacggtggt tccgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
     4381 cccgcagtcc tgaaggtgtc gggccgataa gctgataacg ccacgctacc agcgtcatca
     4441 atcctgaagc aggttgtcgc gtagaaagag gcgcagacga catcgactca ccgcgatgat
     4501 ttagcgtaat tcctacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
     4561 gcggaaacaa taatattgct aataaggttc tcattatttg ccgccaattg tcgccgtcat
     4621 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
     4681 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
     4741 cgcacccagc atctcctgac gggatagcgc ttcctgagtt tgcgccagta aacgatccgc
     4801 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
     4861 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
     4921 ccgtcccaac gccacgcgca cgacggcggt taattcatgt ggatcgcttt ggatgggcgc
     4981 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
     5041 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
     5101 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
     5161 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
     5221 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     5281 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     5341 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     5401 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     5461 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     5521 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     5581 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     5641 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     5701 ggccagcccg agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     5761 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     5821 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgacccc
     5881 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     5941 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca tgttgcagca cgccgaccag
     6001 caacccgccg acaatgttaa tgaccatgat gaggatcccg gcgatggcat cgccgcgaac
     6061 aaacttactt gccccgtcca ttgagccgta aaaatcggct tcctgagtca cttcggagcg
     6121 gcgttttttc gcctcatctt caccaatcaa tccggcgtta aggtcggcgt caatcgccat
     6181 ctgcttaccc ggcataccat cgagaacaaa gcgcgcaccc acttctgcga tacgcccggc
     6241 acctttggta atgaccataa agttgatgat cacgagaatg acaaacacca cgataccgat
     6301 agcgaaattg ccaccaacga ggaagtgacc gaacgcttcg accaccttcc ctgccgccgc
     6361 cgcgccggta tgcccttcca ttaaaatgat acgggttgaa gccacgttaa gtgccagacg
     6421 caacagcgtg gtaaacaaca gaatggtcgg aaacgcagca aactcaagcg tgcgctgggt
     6481 aaacatcgcc accagcaaca ccatgatcga caaggcaata ttgaaggtaa acaacaggtc
     6541 gagtatgaat gcgggcagtg gcagcaccat catcgacaag atcaacagga tcaaaatcgg
     6601 tccggcaagg atctgccatt gtgtcgattt caggtttgcg ggcaggcgca gcatcgcggc
     6661 cagattactc atgggtcggt ttctcgttaa taaaatccag ggcttccggc accggaagat
     6721 gagtaggttg tacagggcgc tgtccaccag ccagacgcca gcgtttcagt tgccagaccc
     6781 aggccagcac ttccgccacc gcggcgtaca gttgacccgg gatttgttga ccaatctccg
     6841 catgtcgata cagcgctcgc gccagcggcg gcgcttcaag cgtcgggacg ttattttcag
     6901 cgccaatttc acgaatgcgc agcgcgacca gccctgcacc tttagcgacc actttcggtg
     6961 cgctcatttt gttttcgtca tactgcaacg ctaccgaata gtgggtcggg ttattgacaa
     7021 tgacatccgc tttcggcaca tcggccatca tccgacgccg tgcagcagct cgctgcatct
     7081 gacggatccg ccctttaaca tgagggtcac cttcgctttg tttgaactca tcacgaatat
     7141 cctgccgtga catacgcagc tttttcaggt ggctgaatat ttggaaaaag acgtcaaatc
     7201 ccaccattgg aatgacacca agcaccacca gcagtgcgca tagccctacc aaatccatcg
     7261 cattacccat ggcggtaatc ggagactcgg ccatcaagcg catcatctgc ggccagtgat
     7321 gccagagaaa aaaccccgtc acgctgccaa ccaggatggt tttcaaaatt gctttaagca
     7381 actccgcgcc agtctgagcc gagaacatcc gtttaatgcc cggtagcggg ttgagtttgg
     7441 aaaacttcgg ctgcaaggat ttgccgctaa ataccagccc tcccagcatg accggagaaa
     7501 tgagcgccac cagcaccacg ccgctaatca gcggcagcag cgccagcatg gcttctctga
     7561 tcagcagaat aatctgcccg aggatcagat tcgggtcatt gataatactg tgatcaaaat
     7621 gcagcccagc ggagagcatg cccgacaatc gacgggccag cgacacaccg ccaaaccaga
     7681 taacactaac gcccaccagc aaaatcagca gtgaggtcag ttcacgggaa cgcgggattt
     7741 gcccctcttc ccgcgctttt tctagtcggt gaggtgtggg ggcttctgtt ttgtcgtcgc
     7801 tctcgtcaga cacgtcgcca atcctggaaa gagtattaag cgtgaatgat gccagagcgc
     7861 aaagcgttca atggtttgag taaggggcaa aacaggcggg atttagggct tttgctgcca
     7921 cacatcaagc atagtgtgcg tttgtcggat gcggcgtcat cgccttatca gaccgcctga
     7981 tatgacgtgg tcacgccaca tcaggcaata caaatcaaaa tccaagacta tccaacaaat
     8041 cgtccacctg atcctgactg gctaccacac cggctttgct ggtatcgacc tgaggtccat
     8101 taagcaaact ctggttttca cgttttggac gcgactcctg ttccgggatg ttttccaaca
     8161 gcaccatcag caactggcgt tcgatctcct gaatgacatc catcatccgc ttaatgacct
     8221 gcccggtgag atcctgaaaa tcctgcgcca tcatgatttc cagcagttgc gcgttagtaa
     8281 agctggtatg cgcgggtaca tctgccagaa attgtcgtgt atctgttacc agttcacggg
     8341 cgtcggcaag gtcaatcgga tcggcaaacc agtcatccca acgttgggtt aacgcttttg
     8401 ctgatttctc catttgatcc tgatgcggtt gtgacgcctc aacactgttc agcgcccgct
     8461 ccgcagcctg ggcggtcatc tgcacaacat agtacaaacg atcgcgcgca tcggggatgg
     8521 cttccgccgc ttcggcaatg gcctgatcca gccccagttc ccgcaaactg tcgcgcagca
     8581 tacgcgtcag gctgccgatg cgcgcaatga tatcgccagc tgaatgctcg tcagcaggtt
     8641 tgattgatgg ttgcatcata gtcgcatcct cacatgccca gtttctcaaa gattttgttg
     8701 agtttttcct ccagcgtcgc ggcggtaaat ggcttcacca catagccact ggcccccgct
     8761 tgcgccgcag caatgatgtt ctctttcttc gcttctgcag tcaccattaa cactggcaat
     8821 gccgacatcg cgccatccgc acgaattgtt ttcagcaatt ccaggccatc catattgggc
     8881 atgttccagt cggagataac aaatccataa ccgcctgcct gcaacttatt gagagcgtcg
     8941 acgccatctt ccgcttcctc aacattattg aatcccagct ctttcagcag gttacgcact
     9001 atgcgtcgca tggtggaaaa gtcatccaca accaaaaatt taagttcttt atccgccatt
     9061 tcacactcct gatttaaata cgtatcgcct gtccggcact aatttttgcc aacatttgct
     9121 ggcttacctg gctaagatcg accacttcgc agacaccacc catattgatg gcctcgcgcg
     9181 gcatgccgaa caccacgcaa cttgcttcgt tttgcgcaag ggtccatgcc cccgcctgac
     9241 gcatcgccaa cattcccgcc gcgccgtcgt tgcccatacc ggtcaggatc accccaaccg
     9301 cattacgccc cgcctgtttg gcgacagaat ggaacaacac atctaccgaa ggccgatgac
     9361 ggttaaccgc cgggccatcg tgaattttga tttggtaatt tgcgccacta cgcgccagct
     9421 ccatatgccg atcgcccggc gcaatatagg catgccccgg caagacacgt tctccgtctt
     9481 cggcttcttt aaccccgatc tggcaaagct tattaagtct gtcggcaaaa gagcgggtga
     9541 aaccgggcgg catatgctgg gtaattaaca gtgccgggct ggaaagcggc aacggttgca
     9601 gtacgtgacg aattgcctca gttccacccg ttgaagcacc aatcgcaatc agtttttcag
     9661 aactcaacaa cggccccgcc ttcagcgttg tcggtgccga caatggctta tgtgctgcaa
     9721 ggctcgcctt tgctgccgta cgcacctttt cagcaatcat ttcgttatac gccagcatac
     9781 cttcgcgaat acccagttgc ggtttggtga caaaatctat cgcccccagc tccagcgcgc
     9841 gcagcgtgac ttctgaccct ttgccggtca gggaagaaac catcacaacg ggcattggac
     9901 gcaaacgcat taatttttcg aggaaatcca gtccgtccat ccgcggcatt tcaacatcca
     9961 gcgtcagcac atcgggattg aatttcttaa tcaagtcacg cgcgaccagc ggatcaggcg
    10021 cggtcgccac catttccatg tcgctatggc tgttgatgat ttctgtcatg atctggcgca
    10081 tcagtgccga atcatcgaca gataacaccc tgattttgct catcgttaat ccttacttag
    10141 cgcatacacc gtctgaccac gcagcgtgaa gcggcgctca aggtggctaa agttttcaga
    10201 gtgacccgca aacagcaatc cgtcgggttt aaggagcgga acaaagcggc gcaaaatctc
    10261 ctgctgggta gtttgatcga agtagatcat gacgttacga cagaagatcg catcaaacgg
    10321 ccccggcacg gtgtactgtt tcgccagtag attcagcggg gcaaaatcaa catagttcgc
    10381 cagctcctga cgcacgcgta ccagcccttc atgcggcccc gtccctcgca tgaaataccg
    10441 ttgcagttgc tgcggcgtca ggtttttcaa ctcttcatgg cgatagatac cgcttctggc
    10501 tttttccagc acttcggtgt cgatatcact ggcaaacact ttccagcgtc cgggcgcggt
    10561 gcccaatgtg tcagccagcg tcatcgcaat gctgtacggc tcttcgccgg tcgaagccgc
    10621 cgcgctccat acgcgatact cgccagaacg gcgacgtgcg tgatccgcga gcagagggaa
    10681 atgatgtgcc tcacggaaaa atgccgtcag attcgtggtc agcgaattga taaacgcctg
    10741 ccactcaccg ctgtgctgat tagattccag caagttcaga taatgaccga aatccgtcag
    10801 tcccagcgaa cgcaaacgac gaaccagtcg gttgtaaacc atgtcgcgtt tatggtcagc
    10861 cagaacgatc ccggctcgtt gatagatcaa ttgacttatc cgccgaaaat gcgcgtcgga
    10921 aagcgccagg cgctcggtca tctgtaacaa taaagacgtt tgcccacagg gcagagatga
    10981 agtcatagcg ccttctcaat cacttcagga taccactggc gcaatttgta actgcaccga
    11041 ctcatgtcgt gctacttcat gttcttcaag ggtaaatacc gccacacgcg acgaaagatg
    11101 gtcggcctga ttcgccagtt gttccgttgc caccgccgct tcttctacca gcgaggcgtt
    11161 ctgctgagtc acctgatcca tctggctgac agcctgtgca acctgctcaa tcccccgctg
    11221 ttgttcttcc gacgcagagg caatttctcc cataatgtcg ttcacgcggg tcaccgaact
    11281 gacaatatcg atcatggtcg cggcggcgtt attcaccagt ttcgaaccct gctggacacg
    11341 attgactgac tcttcgatca gccctttgat ctcttttgct gcctgcgcgc tacggctggc
    11401 aagattgcgc acttcacctg ccactaccgc aaaaccacgc ccctgctctc cggcgcgagc
    11461 cgcttccact gccgcattca gggccagaat attggtctgg aaagcaattc cgtcgataac
    11521 gctgataatg tcgccaattt tttgcgagct ggtggcgatc tcctgcatgg tgtgagtcat
    11581 ggtactgacc tgaacaccgc ccgcctgcgc cgttgtcgcg gcattttttg ccagttccga
    11641 cgcctgtcgt gcgttatcgg cgttttgccc taccgtggcg gttaattgct ccatactggc
    11701 ggccgtttgt gccagcgatg ccgcctgctg ttcggtacga cttgagagat cgttattgcc
    11761 tgcgacaatc tccgcgatac caatgtgcat ctcctggctt cccttacgca catcacttac
    11821 cgtcccacgc aaagcctgct gcatggtctt cagactggca aaaatggcgg tgatctcatt
    11881 acgaccatat accgcaatcg gacgcgccag attacccgca gcaatgctgt caaaatggct
    11941 accgataatg gccagtggtt gaacaatcat cttgcgcgtc caccacagcg cactgctgat
    12001 gtagatcgcc gcaacaataa tcatgctgat aaacaccagt gccgaaatct gatagttacg
    12061 ctggctttgc gcactggcgg cttccagcac atggttgatc tccagctgcc aggcctcaaa
    12121 gttaacgtca aacgcattct gtgatccctg caccggcgca gtgaggaaat ccgaaagttg
    12181 attactttca agccaggtcg cctgatgttc gagatcgttg tgccagcggg caaaactttt
    12241 ctccgtctct ttttgcaatc ccctgacgtg ctcgttgccc gcagtcatcg ccataaaact
    12301 tttaaacagc gtggtggatt gcgtcagact ggcgcgcgcc gtcgtcatca acgttttaat
    12361 gtcatccgcc ggatagctaa gtgccgtcag agtgcccgct ttgttcagcg cggtactggc
    12421 ctgtaacatt accgcccgag tttgcgctaa tgccgcacgt tgctgattgc tctgctcgac
    12481 ctgattcagt cgttgcaaat cgtcgcgaaa tgcccaaaaa gacatgccgt tactgccaat
    12541 ctgcaagatc ccgcagagaa tcaaaattaa aaacagcgtg gtcgaaattc gaatacgatt
    12601 aaacatcaac gctccccatc aggcggcaat gaccgcgtta gtaaatactc gtcaaaatgt
    12661 ttcccagttt ggatcttgtt cagcaattcg cagtcgtggc tgtgccggtg gttgctcact
    12721 ggcaggacgg gatggtgttt gcggtttatt ggtgagtggg ctggctgcca gacggaacgc
    12781 ggaaaccgct tgcgttaaac gactcgcctg ttcttccagc gcagcggcgg cggcagctga
    12841 ttcctgcacc agcgatgcgt tctgttgcgt gacgcgatcc atttccgaaa ccgccaatgc
    12901 gacttgatcg atgccacggc tctgttcatc cgatgccgat gcaatctcgc ccataatgtc
    12961 agtcacgcga gtgacagcat tgacgatatt gttcattgtt tccccggcgc tttcgaccag
    13021 caccgaaccg gtatcaacgc gtgagacgga gtcttcaatg agggctttga tctcttttgc
    13081 cgcctgggcg ctgcgactgg caagattacg cacttcaccc gccaccacgg caaaaccacg
    13141 gccctgttca cccgcacgcg cggcttcaac cgcggcattc agcgcgagga tattagtctg
    13201 gaaggcaata ccgtcgataa cgctgataat gtcggcaatt ttcttcgaac tatcggcgat
    13261 ctcatgcatc gttttcacta cgccatccac cactttgccg ccgtgctggg cggtgtcgga
    13321 ggcactttgc gccagttgcg aggcctggcg ggcgttatcg gcgttttgct tcactgtcgc
    13381 ggtgagctgc tccatgctgg cggcagtttc ttccagcgcg gatgcctgct gttcagtacg
    13441 ggaggaaaga tcggtgttgc ccgccgcaat ttcacgggta ccggcataga tggcatctga
    13501 accttcgcgg acatgagtga cggtgtcagt caaagagcgt tgcatatgtg aaacgctctg
    13561 cgccaggtcg cccatttcac tgcgcccgtc aatggtcagg gtattcgcca ggttaccacc
    13621 ggcgatttcg cgaatgtgag caataatttt tgccagcgga gtaagcaaca tacggcgaat
    13681 gccgtaccac gccaccagca gaatcaatac caccaccagc gcgataaccg ccagttgcca
    13741 ctgggcaaat cggtaatcat ctgcgttgtc agtgacgata tcgcgataca gtttttcact
    13801 gctgagggcg tactgagcaa acgcttcgcc cattgcattt tgcattccct gggttggctg
    13861 agcgaaataa gctccagtat tgccataatc tagataatca atcagttcag ttaacgctgt
    13921 gtaatagttt ttatattttt catcaatatt acgactggta gcgaccattt caggtaacgg
    13981 tgccatgctt ttgaattttt tataatgcgt cgctgcctgc gccaatgttt tcctggcgct
    14041 atcgagcaat tcaactttgg cgttactttg ttgattggag gaatccatca tcatccgtac
    14101 cgctgaacga ctcaggttaa tgcgcgtttg cagcattaaa tcccaggttg acgtcagctc
    14161 gccctgctgt tcccgtaatt gattggaaac cacaaagctc ttctggctat ggtgaaggga
    14221 agaaaaaaac agactgccgg aaataagctg taacagtgcg aataccccca gcaccattac
    14281 caacagcgtg actacgcgga tacggttaat cataaggcac cttcctgaaa acaagttgat
    14341 ctcgttatcg gcaaggaggg gggaaacttt attgctgatg ccacccgccg cgaaattgaa
    14401 ataaaaaacc cgatgcgcag atcatcgggt tcatttcaat tgaggaaatc gggagaatta
    14461 cgccacttct gacgacgcgc tatctaacag cgccatctct tcgctgttca gcagtttttc
    14521 gatgttcacc agaatcaaca tccggtcgcc cagtgcgccc agtccagtga gatattctgt
    14581 tgaaagcgtc acggcaaatt ccggtgccgg acgaatttgc tccgccgtca atgaaagcac
    14641 gtctgagacg ccgtcaacca cgatgccgac cacccgctgt ccgagattca ggacgataac
    14701 taccgtgttg tcgttatagt ccacatccac ctggctgaac ttaattcgta agtcaacaat
    14761 cggcacaata acgccgcgca gattcgtgac gcctttgata aacgctggcg tgttcgcaat
    14821 ccgtgttacc tgatcgtagc cacggatctc ctgcactttc aggatatcaa taccgtactc
    14881 ttcatcacca agggtaaata ccagaaattc ctggcctgac ggctcgctgg ccagctttgt
    14941 tacattcgtc ataccggtca tattgttacc tttttactca ttcaggcggc ggtgttcgcc
    15001 atacgttgtt cgcggtttat cgcctgcaag gcggagacat caacaatcag tgccacgctg
    15061 ccgtcgccaa gaatggtcgc agcagaaatg ccggggactt tgcgatagtt actttcaagg
    15121 tttttaacca caacctggtg ttgaccaatt aattgatcca ccagcaaggc gtagcggcga
    15181 ccgccacttt gtaagatcac cacaattccc tgggtggctt cggttttcgc gcccgcgacg
    15241 ttgaacactt tccacagttc gacgatgggc agatattcac cccgcacttc cagcacccgc
    15301 tcgccgccgg ccagtggatg gagatcggct tcacggggtt gcagtgattc cataacagca
    15361 ttcagcggca gaatgaaaac ttcatccgca acgcgtacgg acatgccgtc gaggatggcc
    15421 agcgtcagcg gcagtaaaat gcggatcgta gtgccagtac cctgcttcga ctggatttcg
    15481 acatgaccgc ccatctcctg gatattacgt ttaacgacgt ccatgccgac gccgcgcccg
    15541 gagacgtcgg tgacctgctc tgccgtggag aagccaggtg caaatatcag catcgcgact
    15601 tcgtcgtcgc tcatgttttc gctgacagtc aaaccttgcg aggccgcttt tgccagaatt
    15661 cgctcacggt ttagccccgc cccatcgtcg gtcacttcaa tgcaaatgtt gccgccctga
    15721 tgttcggcag acagaattaa atttccgacg ctgtttttac ctgcggcgag ccgtttttct
    15781 ggcagttcaa taccgtgatc gaggctattg cgtaccaggt gggtcagcgg gtcgataatg
    15841 cgttctatca ggcttttgtc gagttcagta gaactgccca ccagcgtcag ttctacctgc
    15901 ttgccgagtt ttcccgccag atcacgcacc agccggggat agcgactaaa aacatattcc
    15961 atcggcatca tgcgaatcga catcactgat tcctgcaaat cacgggcgtt acgttgtaac
    16021 tgccccatgc tggttatcaa atcaccatga ttaaccgggt ccagttcgct ggaacgctgg
    16081 gcaagcatgg actgggtgat aaccagctcg ccgacgaggt taattaattg atcaaccttt
    16141 tctaccgcta cacggatgct ggtggattca ttgctgcgcg tcgttttttc ccgctccacg
    16201 cggccggttg gcgcttgttc ggctgccagt ttaagcactg gtggggtgga tatttttggc
    16261 gagacttcta ctgtttcaaa ggtaatctga tcggcttcaa tcacaaaaca gagtaccgct
    16321 gtgatgtcat cttcggcgat gtcgcccggt aatattgccg agagcgaatc cgcccctttc
    16381 accacgtcag ttaacgttgt cagatgtccc agttcttctt ccagcaggtc gacttccccg
    16441 gccttcaggc gcgaaaggat aattcgtcgc ggcgactgac tgcgactctg ctcatcttgc
    16501 ggttcacttt tggcaaccac acttaatcgg gtcactgcgg atggcgtttc gcctttcgct
    16561 tctaatgcca gttgacgcaa ggcctggcag atataatcga agctggcggc atccggctct
    16621 tgcgactgtt tataagcgtc gagctgttct tgcatgatgt ccttcgtttc caaaaacaga
    16681 ttgataatgt cggtgttgag ttgcatctca cctcgtctgg cttcatcgag caggttttcc
    16741 atcagatgcg tggtttcctg caaaacgctg aagccaaaag ttcctgcccc tcctttgatc
    16801 gagtgggcag cccgaaagat ggcattcaat tgttcggcat ctggcgcttc cggctgcaaa
    16861 accagcaaat gctgctccat gtcagccaac agttcgtccg cttcatcaaa aaatgtctga
    16921 taaaaatcgc ttatatccat gctcacgctg tcacctcggt tcggctgatg gcattgtggg
    16981 aacactgacc tgtggtgcaa cctcaggttt ttccagggcg cttactggct cattctggct
    17041 ttcggcgttt tcatgcaaaa tggcctgttc ggcttgtttg ttcagtacca gcaggctgat
    17101 gcgacggttg acggcatcat caggtccgcg atcgcttaag cgcatcgttg ccgccatgcc
    17161 gacgacacgt aacactttgc cgctatccaa ccctccgacc atcagttcgc ggcgggatgc
    17221 attggcccga tcggcagaaa gctcccagtt gctatatcct ttctcaccgc tggcgtaggg
    17281 gaaatcatcg gtatgacctg aaaggctaat acggttggga ataccgttca gtacaggcgc
    17341 aatggcgcgc agaatgtcgc gcatataggg ttcgacatcg gcactgccgg ttctaaacat
    17401 cgggcgattc tggctatcga tgatctgaat acgtagacct tcctggacca gatcgatttt
    17461 gagatgggga cgtaacgccc gcagtttcgg atcggactct atcaactggt cgagatcacc
    17521 ccgcaatttc cgcaatcgac tttgctccat gcgttttttc agctcttcga tgttcggctg
    17581 cttattcact tccccctggc tttgggtgta atcatcacca ccgccgggaa ttgggctttc
    17641 actattagaa atgcgatcgc cgcccgtaac cgcagtcgcc agtggagtcc ggaagtactc
    17701 cgcaatctga atcagctctt ttgggctgga gatggagatc agccacatca ccagaaaaaa
    17761 ggccatcatc gcagtcataa agtcggcata agcaatcttc cacgatccat gtgctgcccc
    17821 gtggcttttg gctttgcgtc gtttgacgac aataatcgga tgcgcttgat tcttcatgct
    17881 tcctcggttg tcgtctgttg ttgcggattt ttcaccgcac gcacatgctc ttccagttca
    17941 ataaacgacg gacgttcgct ggaatagagc gttttgcgac caaactcaac ggcgataggc
    18001 ggtgcgtaac cgttcagatt agaaagcaga gtgactttga cgcactgcat cattttgctg
    18061 gtttcggcgc ttttctgacg taaaacagtc gctaatgggg aaataaatcc gtaagccaat
    18121 aaaatgccga ggaaagtccc caccatcgca tgtgcgataa gcgcacccag ctcggcggca
    18181 ggacgatcgg ctgaacctaa cgcgtgaacg acccccatta cagccgcaac aataccaaac
    18241 gccggaagtg agtccccgac cagcgccaga ctgttcgccg ggacttctgc ctcgctttcg
    18301 tgcgtctcaa tctcttcatc catcagggct tcgatttcga aggtgttcat gtgaccgctg
    18361 ataatcaggc gcagataatc gacgataaaa tcaagcatga cgctatccgc gaggatgcgt
    18421 gggtagctgg cgaagatctc gctctcacgg ggattttcaa tatcacgttc cagcgaaaac
    18481 atccccatct gccgcgattt tgccatcaac cgataaagca gagccagcag atccatatac
    18541 attgctttgg tgtatttgga gcgacgaaac agcaacggca gcgccttcag cgtgccttta
    18601 atcgctttgc cattattgcc gacgataaac gacccaatcc ctgcaccggc aataatcacc
    18661 agttcagcgg gttgatagag tgctccaagg cttccaccgg tcatcaaata accgccgaaa
    18721 actgtaccga gaacaaccag gtaacctaat aagataagca cgacatcatc cttccactgt
    18781 tgaccatgac aggatgttca gtcgtcaggc gttaacgcgc gattggggca aaaaaaagca
    18841 gcggtacgtc gttaccgctg ctggaatgtt gcgcctcacc gtatcagtta aacagcctgt
    18901 actctctgtt catccagcag ttgtgggata atatcggcag gattctggga aagtttacgt
    18961 ctttttactg ccctggatgg cggttgacat aagctgcagg caaagctgcc aacaggctgg
    19021 tgagcgtggg taataaaatt gccgccgcag cagttgcagc tggaaagttg cagtaatcca
    19081 ctttcaacaa accgcaccaa tgtccaggca cgagttaatg ccagcagtgg tccttcttct
    19141 gcttgtgggc actgttcaag gtataaacgg taggctttga tcaccgcatc gacgccatta
    19201 cacaaaccgg ttttcagtaa aaactgccat gcattacaga acatcgaagc atgaacgttt
    19261 tgttcccagg tcataaacca gtcggttgag aatggcagca tgcctttcgg cggtgggctt
    19321 ccgcgcagtt ctttataaag ttttatcagg cgtccgcgac ttaactgtgt ttcgctttcc
    19381 agcatctgca aacgagcgcc cagggtgatc aattccattg ccagctgaat atcccgcgct
    19441 tcctgaacaa tgcttttttc actcatgatc aggccctttt cttgcgcagc gcttcttcag
    19501 gctgattaac atcattcagc aagcgtgttg agagcatgat gccggtatga atttgctgga
    19561 gatcgtcaac gcgggaatct tgcgtcaact gagtaatcgt ctggtggctg tcaaaacgga
    19621 agtgacaaac cagctgattg gtttctgcca gcttaaccat ttgcggaaga gtcagtgccg
    19681 ctaatgttgt cgccatttct tcatttatgc cgagacgaaa catagcggac gctttgtcct
    19741 gaacaatcag acgctgtgca agtagtaaat atgacaagtt gatgtcataa atgtgtttca
    19801 gcaactcgga ggtatgcatt attcccaccc agaataacca actttatttt tatgcggttt
    19861 caccgcaccc tgtgatgtcg ccgggaagcc ccggtaaaaa taattagcat tagaatagtt
    19921 gcgataagct gcaataagca gagccacctt tttggtttaa taagtcctta caaatagaag
    19981 ggggtcttta cacttatcta agatttttcc taaatcgacg caactgtact cgtcactaca
    20041 cgcacataca acggaggggg gctgcgattt tcaataatgt gtgatgcaga tcacacaaaa
    20101 cactcaatta cttaacataa atgtataagt catacttttg ttttgggtgt atttcaatct
    20161 gttaaaaagt ttttcgctac gctagcaagc aaaaatgaaa caggaat
//
LOCUS       DBANUU010000083        20075 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000083 DBANUU010000000
VERSION     DBANUU010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.083"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(303..866)
                     /locus_tag="ACIE68_003002"
     CDS             complement(303..866)
                     /locus_tag="ACIE68_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFX5909283.1"
                     /translation="MALYNFTLTLSGVSYETEGLEDALYQNGCDDALICAYGNSVYVE
                     FDREAQSLDAAIASAVDNIESAGIGAIVESVDSALVGLSDIAELTDLSRQAVALLKDG
                     KRGSGDFPCPVQRIKGQSPLWDWADVAEWLMNNGRLKEGDQIVQNARTLSKWNLALRN
                     SVSKDFNEIEQIAEKLIKRRKDIAKCA"
     gene            complement(927..1151)
                     /locus_tag="ACIE68_003003"
     CDS             complement(927..1151)
                     /locus_tag="ACIE68_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909284.1"
                     /translation="MKQHPNKHIQAAIDYALSKGWIWVAAGGAAHCFCRLRCGNPEGE
                     HKTHQMSVWSTPGNPENHAKQIKRMVDRCK"
     gene            1406..1837
                     /locus_tag="ACIE68_003004"
     CDS             1406..1837
                     /locus_tag="ACIE68_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909285.1"
                     /translation="MNTDIFSKIMADLEFDRDNLEEVWRKQPRLLMEYGSKLAQADRD
                     VAEAKLNLEAVEAKLYDTERKNLSMNGIKFNESVLDAKVKTNPQYLSKRQKLDEARHI
                     ADIYKHAVAAFSHRRDMIVQASKMAIVELERLGSERFITPR"
     gene            1955..2983
                     /locus_tag="ACIE68_003005"
     CDS             1955..2983
                     /locus_tag="ACIE68_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFX5909286.1"
                     /translation="MSKTLLDLLNKTREDIAAKRGNNVDLTRLKDGVNYIRIFPNKDD
                     PNGKFFQTFGMHYVKYQNEEGKEATNAYICEQHTHGRACQLCEMVMEGRARHKGNKAM
                     EERIGQMRATPRYLVNGILSAREDFADAEKCQLIELPSTVFDDICKAITEDIADDIGN
                     PLSKEEGYAFLIKRTGSGRDTKYDVSPKRKVYKGDIEDKFWNTQHDLIAYANQADETR
                     LLSTVRTMGRLIGIAAPTAAASAPAISSTAKTSAAALPGFGSVTGHTEGATAVATAHT
                     PASEPTSLVDEEILRAVETEFKPEASSAAVAVSVKESEAVAATSVATASATEDEGLDD
                     LLRELDSL"
     gene            3044..3988
                     /locus_tag="ACIE68_003006"
     CDS             3044..3988
                     /locus_tag="ACIE68_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HFX5909287.1"
                     /translation="MNYLFVDGNSLGYYHQQSDKLHNGEMEVQAAFGFVKNVRRYASI
                     LHARPMILWDGFSDKRRDFYPDYKANRDDDPDMKKMKEGFAIQKPYILKMMTALGVTQ
                     LIAKDAEADDLAGLLVSRMAPQPTVEHIYLLTGDSDWLQLVRENVSWVSLREDAKNKQ
                     VNFEQFAELTGFATPRAFLEAKALQGDNSDNISGVGGIGAGGAKELLHEWGSVATMVR
                     GINDGSIVVNKGRHKTAFNKLAKNAFNEKTGCRMLEAFKRNITLMNLIETKFPPTEIE
                     TIKGNRDVKAFEQLCYELNFRSFLEDLEVFVLPFERYC"
     gene            3988..4254
                     /locus_tag="ACIE68_003007"
     CDS             3988..4254
                     /locus_tag="ACIE68_003007"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909288.1"
                     /translation="MLKSIINGATTTPAQLAKEIVFYHGEYAVIALPSILGAAGMKAT
                     DREFGLVSEQVVKILARVSRLLNHDAIVFDESAALKRINETKGA"
     gene            4257..5330
                     /locus_tag="ACIE68_003008"
     CDS             4257..5330
                     /locus_tag="ACIE68_003008"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFX5909289.1"
                     /translation="MAKGKSALALALKKKIGSNDEIQKVTHWIDTGFPPLNKAISGRY
                     DGGFPCGRIVEVFGPPSAGKTFLATAAMISAQKQDGLAVFLDHENSFDVGLAVANGLN
                     ADEDDGQWVYKQPDTFEDSVELIGTILKLVRDEELIPESAPICIVADSLASMVPNSKA
                     EKFEKMAEGTAKDKDQLNMNDNTALARATSANFPTLALWARKYNACIIFLNQVRTKIG
                     VMFGDPTTSPGGDSPKFYASVRIRLGASVMKDGKEKIGQDVGAECIKNKVAPPYGKCT
                     WKFYFDPTRGLDVIESLVEYMLEEGYLPKNASGRVEIGDKKYTKSQIVEMYREKPLAE
                     IIAALQAIDDRRAKDNPTESVEE"
     gene            5423..5623
                     /locus_tag="ACIE68_003009"
     CDS             5423..5623
                     /locus_tag="ACIE68_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909290.1"
                     /translation="MIKIYLLAVATGLSVALIYGLLVPSLISTKSDLAVMFGVIVGFG
                     APVIGLIAGRKFINSLIKAKGK"
     gene            5627..6457
                     /locus_tag="ACIE68_003010"
     CDS             5627..6457
                     /locus_tag="ACIE68_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFX5909291.1"
                     /translation="MKKGLLAVALAAICTMGLTGCDRVEPGYVGIKVNKLGEDKGIGE
                     VVGVGRQWTGLNTELYVFPTFKQMKTYDEPFTFQMSDGTAIGHKIGVAYLVNRDKVTT
                     VFQTYRKGVDDITESDLRQKIADSLNRLASRMTTDSFIDGGKAQLLDNALKDIQKEMS
                     PVGIEVLSLSWVGKPDYPKTVIESINAKVTANQRTLQRQQEVEQRKAEANMLREQANG
                     EADAIRARAQAEADAIRLRGEALRQNPNVMELEAINKWNGQLPQYMTQGANTPFITVK
                     "
     gene            6549..6974
                     /locus_tag="ACIE68_003011"
     CDS             6549..6974
                     /locus_tag="ACIE68_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909292.1"
                     /translation="MTAIKKLYDAANVALDVIDDEAAKGFPEPDWAHQLRNAIAEMNP
                     PDPTTDETDWQRFIRMYAQEIGPTPTAEQAMLLKYFKEAGEDLPIDDSAYWFHCAWRK
                     YDVIFTQGMGSKDMVVWHLLHIDTAVDRVIEQFFPKQED"
     gene            7030..7305
                     /locus_tag="ACIE68_003012"
     CDS             7030..7305
                     /locus_tag="ACIE68_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909293.1"
                     /translation="MRVLVRIVTSTVYDVFPLFMVKADGLNDEETDALIQRILVEYTG
                     HDADSVMVDDDGVCWHNGNCWYVEETQQISDEDAAHLERILSISTFE"
     gene            7521..7856
                     /locus_tag="ACIE68_003013"
     CDS             7521..7856
                     /locus_tag="ACIE68_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909294.1"
                     /translation="MKAIPFALLFLSSIVVADTTVYQCEMSVADVKNGALTDVIKAPY
                     GAMVVDSGDQFYVVRDDRVLSSPYLTNRNGKLTGVGEDHFVYNKYKGFYGVHASQQSY
                     LFDDCKEVG"
     gene            7856..8068
                     /locus_tag="ACIE68_003014"
     CDS             7856..8068
                     /locus_tag="ACIE68_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909295.1"
                     /translation="MALTLAGLEIEKTSGYWRAKGFKQPGILERLEREDGYIVHQRRE
                     WRMYDPETGKLTTKAGTLWGLLKKIH"
     gene            complement(8648..9862)
                     /locus_tag="ACIE68_003015"
     CDS             complement(8648..9862)
                     /locus_tag="ACIE68_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFX5909296.1"
                     /translation="MLSAVIDSLSAVHRMLPTVFGILSAANQVCCQRLNDKGVHMSTK
                     NKKGKNNKEVEDNLDNFEEGSLELYTGELVPNSNNTVQPIALMRLGLFVPTLKGTKYS
                     KRNKPNEIDASKELVQLEVARSEGYSDIKITGPRLDMDHDFKTWVGVVRSLAEYGEPN
                     GRVELSITKFAKFCGYPSSQIRKTLRDRLTNSLLKIMRTTLSFQRTYEEKNVDGSNKI
                     SLLMVHLINSVDYNEQKDTVVFYAEPKLAELYRFDHKVLLQLKVINKLPRKETAQALY
                     TFIESLPTKPAPVSLARLRARLNLSSRNVSSQNQTIRNGLKALQDLGYLEYSEIKRGR
                     SIYIQIHSRNPKLKVAPPKPEDIEPKKPDEKAGEIDAKQNIINKITELSQNLTPENIK
                     MIEILSNSLKLL"
     gene            10064..10708
                     /locus_tag="ACIE68_003016"
     CDS             10064..10708
                     /locus_tag="ACIE68_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909297.1"
                     /translation="MDLKRTRWVRRLEDGSYTIESNSNLNKQKLLCDICGIAAKCPIY
                     ETRIKLDKAGVNFHLNSCIRYVPLLAFRKPIIGLDAPYFNTLRSGVTWRDRLSPDKLI
                     CLVSADTGKIIRFGKVDKVYSGPVDEMLRKHSRFNHLCMGGEKIEKVEEVIRKSYGHF
                     LTKDSLLTAIYIRHVKREFDLEYHSEEELNLVDPRPKAGVISINAARKKPTDAL"
     gene            10963..12048
                     /locus_tag="ACIE68_003017"
     CDS             10963..12048
                     /locus_tag="ACIE68_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFX5909298.1"
                     /translation="MTLPYGVISDCHYHKWDAFSTTNAEGLNSRLEIQLEATKEAAIA
                     MKKAGCKYMLVAGDTFHVRGTVSPSVLHYVTETYKWIINELDLTVVMLAGNHDLETND
                     SVYSANAAASLSSIGVVIVCGKRPHSIKIGDVTVHLISWRNNHAELISDLKALRKSVE
                     GDNHDVVIHTSINKAIPTMPDVGIDAQELKDIGFRLVLSGHYHNHKEVIPGVISVGAL
                     THQNWGDVGSLAGYMIVNPDGSFSHYETSAPKFINLEDDVADDQIRGNYVRFRAVIEN
                     DEEGIKYQNILKTMGAKGVVCNFIRKSSMMEGTASTTETSKIDSLGESVSAYCKIVHD
                     TDGGFDLSKLDILCQEILTEAESSEAV"
     gene            12278..14194
                     /locus_tag="ACIE68_003018"
     CDS             12278..14194
                     /locus_tag="ACIE68_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFX5909299.1"
                     /translation="MKFLKLQVENFMAIASAEVELDQRGLVLIQGVNSDDSSASSNGS
                     GKSTLMNSLMWCLYGETAHGVKGDDVLSTDHEKNCRVAVTIEDEGKRYAIIRHRKHKE
                     FKNRLIVRGEDGDMTKGKDALTQEFVERLIGASKEVFMASIYASQEAMPDLPGMSDKN
                     LKTIVEEAAGVDRLTRAYAIARERANAAAARMDVVKTKLESTISTIEATQSEIESAKA
                     SSESWEQERSKRYDDALAGLASAEVELTEVELEIRTLPEQIRDTEKAIESERKKLASK
                     EEHDAKLLKVRGAITDIRASIKATENSQADAMSRARNFKTKAEEVGTKVGSPCPTCGK
                     AYCEEDLSTVKENFIEQARQEIGQAKTLAEAMAKHKTNLEKALSIESALVKTTPDVTA
                     IIARIEELTKQLSSLRHREKEVVAIESLVTRARTEVDRISKEINPFIALIARHEDNLV
                     SSKSTFKSLKDELKAIQEQTLLLEKARQVYSPAGVRSHILTSVTPFLNTRTAEYLNTL
                     SDGNITAEWSTMDVTKKGEYRDKFNISVQKKGSSKSFQTLSGGEKRKVRIACSLALQD
                     LVSNRASKNIDLFIGDEIDDALDTAGLERLMGILESKARERGTVLIISHKEMKSWFRE
                     TITLEVKEGRSYVV"
     gene            14184..14927
                     /locus_tag="ACIE68_003019"
     CDS             14184..14927
                     /locus_tag="ACIE68_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909300.1"
                     /translation="MSFKLSRSQFLQVFAVMQSIKLINGHTSNGAAPRILWGSNNIDG
                     VQFAALLGLISETPLMQSLKSLPPGCIAPILINPFVEGGYLPNVGPGFIASHETEDLN
                     INSEGVFGGMGAHHCMAFTNLIRLANKRVDSLASPGDAFTGFLIQRRDKKYSADKLQF
                     VGKYGEMVEIELQLPHVLANDSADSRRLLGIMRHFIASGVKHAADKRVTQENEYSDFA
                     NYPQPTLQTAIVANSLEARLLENPIWGTW"
     gene            14937..15506
                     /locus_tag="ACIE68_003020"
     CDS             14937..15506
                     /locus_tag="ACIE68_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909301.1"
                     /translation="MSKKISVVGVDPSMSNFGLAVGTLDLETDELEIHGLTLVETKAG
                     SNKKTVRVNSDDLRRASEIWRVAKPIIDKANMVFCELPVGSQNSRSQTSYGICIGVLA
                     CVDKPLIQVTPNEIKHFVGNKLTTSKEEIIQWATKKHPKAPWLRRKQSGQDVLVNKNE
                     HLADAVAAIHTGMQTDQFRQVRDVLKSLI"
     gene            15580..17895
                     /gene="nrdA"
                     /locus_tag="ACIE68_003021"
     CDS             15580..17895
                     /gene="nrdA"
                     /locus_tag="ACIE68_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFX5909302.1"
                     /translation="MISIVKRNGQTEPLSEEKYNRVVMYGVEGIRGVSASAVAMGAAA
                     SIFDGITTSQLHEALVKSAADLISPEAPNYSQVAARLNIFKIRKDAFGRYDYPNFYQH
                     IVKNVNKGVYDKDLLTHYSFEEIEELGNYIKPKRDDLFGYAATVQLQSKYLVQNRVTG
                     EIHEGPQHIYMLVGMCLFQNWEDDCAGKTRMEMVKGFYDVTSTFKLSLPTPIMAGVRT
                     PTRQFSSCVLIESGDSLKGISAASAAIIDYVSRRAGIGIGFGRIRALGSEIRNGEATH
                     TGVIPFLKHFQTAVKSCSQGGVRGGAATAFYPIWHLEVESLLVVKNNRGIDENRVRHL
                     DYGVMSNRLMYRRLVRSENITLFSPHDVPDMYEAFFTDQELFEKLYHKYEADDSIRKK
                     PVPAIELFSSLMQERASTGRIYIANVDHINEHGAFIPALAPVRQSNLCMEITLPTRPL
                     AFTDDPNGEIALCTLSAFNLGAIRSLESLKEVAFYAVAALDSLLDYQDYPMEAAEVPA
                     KARRSLGIGVTNFAYYLAKNGVRYSDTAGNKLVHETFEAIQYYLLDASCRLAEAKGEC
                     DWFEQTKYAIGQLPIDHYRSSLDESGETNFELKMPWEELRERIAKYGLRNSTLTAQMP
                     CETSSQITNSTNGIEPPRGPVSVKSSKDGIVKMVVPEFEKLKEQYEYLWDMPDNRGYL
                     TKVAIIQKFFDQAISANTNYDPSRFEGDKVPMMTLLSDLLLAYKMGVKTLYYHNTRDG
                     AGKRDDDEPQNPLTQAVAVEPEDECDGACKI"
     gene            18002..19144
                     /gene="nrdB"
                     /locus_tag="ACIE68_003022"
     CDS             18002..19144
                     /gene="nrdB"
                     /locus_tag="ACIE68_003022"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFX5909303.1"
                     /translation="MSYSTFRLGANDATKEPMFLGQSVNVARYDQQKYRDFEKLIERQ
                     LSFFWRPEEVDISSDRIDFNTKLRDHERHIFLSNLRYQTLLDSVQGRSPNATLLPLIS
                     IPELETWVETWSFSETIHSRSYTHIIRGMVDDPSIVFDGIVTDEEIINRAISISAEYD
                     RLYGMTCERQSLGEKEFERLYVNEYGWEPYPLHRQLFRTLVSINALEAIRFYVSFACT
                     FAFGERKLLEGNTKIMRFIARDEALHCEGTERMIRFMRTGREGLLWKEIAADEENVIY
                     DTMKSVAEQEMNWADYLFKDGSMIGLNADILKTYVKYRTNLAMNRLGLKALFPEVTTD
                     PLVWMNKWLLTDTLQIAPQEAEQSTYLVGQIDSTVDKASLSQFADL"
     gene            19222..>20075
                     /locus_tag="ACIE68_003023"
     CDS             19222..>20075
                     /locus_tag="ACIE68_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP pyrophosphohydrolase"
                     /protein_id="HFX5909304.1"
                     /translation="MNFTKLTDHLKLATDRLIGFKPEPYELHEGHGVATESIYKMVDQ
                     FHELFQHPRRVMPTPELLRLRASLIHEEAVVEGIPAAMNGDIEQLLDAMADFLYVGVG
                     TMVAIKGGISTGMTYYTQEQSIDRFMQTIFVPGNTVFDDMAMPFQEAREASCMLEELA
                     DKLENKTVKDSELIQELRRVMNKIYVACMMTYRLADFLGINVVELVGEIHRSNMTKLW
                     PADVEERRQAVANCKYDSSDLGFRHADGTDKMIGFRISDGKILKSPTYSDVDLSSFVE
                     QAKASAMYG"
BASE COUNT         5620 a         4517 c         4784 g         5154 t
ORIGIN      
        1 cacatagtgc ggcgagaatt tgatctgtat agattgggac ttacttaggc acttaaggag
       61 gagactcacg aagaagggaa atcagaagat aacactgaac tgagactgct ttactaaaac
      121 tgcttacata aggtctgtct tgcaaaccaa cgatgctatt aacgcatacc ccttcttttc
      181 atgcaacctt tatttttcaa agataactaa tttttgtcac cattggttta gtaaacgtta
      241 acgaaccaga cgatgccaga cacctttaca gagaacagat gcccgccaat tggcgggcat
      301 cattatgcgc atttcgcaat atctttgcgg cgtttaatca gtttctccgc aatttgctct
      361 atttcgttga aatctttcga gacgctgttt cgaagcgcca gattccattt actcaaagtt
      421 cgggcatttt ggacaatttg atcgccctct ttaagtcgtc cgttattcat gagccattca
      481 gccacatcag cccaatccca gagaggagac tggcctttta tgcgttgtac agggcaaggg
      541 aagtcgccgc ttccgcgctt accgtctttg agcaacgcca ctgcctggcg agacaggtct
      601 gtcagttccg cgatatcgct taagcccaca agagccgagt cgacggattc aacaattgca
      661 ccgataccgg ctgattcaat attgtcgacc gcagatgcga tagctgcatc aagtgattgt
      721 gcttcgcggt caaattctac atagacggag tttccatatg cgcaaattag cgcatcgtca
      781 cagccgtttt ggtacagcgc gtcttccagt ccttccgtct catacgatac gcctgagagc
      841 gtcagagtga agttataaag cgccatagtt ttcctttgaa gatagttgga cagtttctgc
      901 aaaaggcggc taatgccgcc ttgaaatcat ttacaacgat caaccattcg tttgatctgt
      961 ttggcatggt tttcgggatt acccggagtc gaccatacgc tcatttggtg agttttgtgt
     1021 tcaccttctg gattaccgca tcgcagtctg caaaaacaat gtgcagcacc accagctgct
     1081 acccagatcc agcctttact taatgcatag tcaatggctg cttgaatatg cttattcgga
     1141 tgttgcttca ttcgcctccg ataatagtat tctattcaca gtgttgacat ctgtcaacgg
     1201 cgactgaatt tcatccgttc atgccacacc cccatccatc tcatgtatgt gacctaaaga
     1261 tggtcagcac agcttaccaa aataagacaa ctaattatct tcggtgatct caatattttc
     1321 ccctgaacaa agttgacatg acgccataaa tccgttctct gtttgatata attgatacag
     1381 attttataag taggaaccta ttagagtgaa cactgatatt ttttctaaaa tcatggccga
     1441 tctggagttc gaccgcgaca accttgagga agtatggcgt aaacagccac ggcttttaat
     1501 ggagtatggg tcaaaactag cgcaggcaga tcgagatgtc gcagaggcaa aacttaacct
     1561 tgaagctgtt gaagcaaagc tatacgacac agagcgtaag aacttgagta tgaacggcat
     1621 taagttcaac gagtctgtac tggacgctaa ggttaaaaca aacccacagt atctgtctaa
     1681 acggcagaag ttggatgaag cacggcacat cgcagacata tacaaacatg ctgtcgccgc
     1741 cttttcgcat cgccgagaca tgatcgttca ggcgtcaaag atggccatcg ttgaattaga
     1801 gcgattaggc tctgaacgct ttattactcc ccgttgattt ttgatagata ataagtaagt
     1861 actgatctat catttaacag ctcgaaagag ccacgaatga acgaaagccc aacgcgcata
     1921 gcgccatcgg ccaaatcaca acaaggagaa acacatgtct aagacattac ttgatttgct
     1981 taacaaaact cgtgaagaca ttgccgccaa acgtggcaac aacgttgatc tgactcgctt
     2041 aaaagacggc gtcaactata tccgcatctt cccgaataaa gacgacccaa acggtaagtt
     2101 cttccagact ttcggtatgc actacgttaa gtatcagaac gaggaaggta aagaagcaac
     2161 caacgcttat atttgtgagc aacatactca cggtcgcgct tgtcagctat gcgaaatggt
     2221 tatggaaggt cgcgctcgtc acaagggtaa caaagcaatg gaagaacgca tcggtcaaat
     2281 gcgtgccact cctcgctacc tggtcaacgg cattctttct gctcgtgagg atttcgcaga
     2341 tgctgagaaa tgccagttaa tcgagctgcc gtctactgta ttcgatgata tctgcaaagc
     2401 aatcaccgaa gacatcgctg atgatatcgg caatccactg agcaaagagg aaggctacgc
     2461 attcctgatt aaacgtactg gctctggtcg cgataccaaa tatgacgtct cgcctaagcg
     2521 taaagtctac aaaggcgata tcgaagataa attctggaac acccagcatg atctgatcgc
     2581 atacgcaaat caggctgatg aaactcgtct tctgtcgaca gttcgcacta tgggtcgtct
     2641 gattggcatc gctgcaccaa ctgccgcagc atctgcacca gcaatttcct caaccgcgaa
     2701 aacatcggct gcggcactac ctggatttgg ctctgtcact ggtcatacag aaggagcgac
     2761 ggctgtagca accgcgcaca caccggcttc tgaaccaacc agtctggttg atgaagaaat
     2821 cctccgtgcc gttgaaactg aatttaaacc agaggcaagt tccgctgccg ttgccgtatc
     2881 agtcaaagag tctgaagcag tcgcagcgac atctgtagca accgcatctg cgacggaaga
     2941 tgaaggtctg gatgacctac tgagagagct ggactctctg taatcccatt acgtgaccag
     3001 taaggcgtct acggacgcct tactttttgg aaggaatgta ccggtgaatt atctcttcgt
     3061 agatggcaat agcctgggtt attaccatca acaatctgac aaattgcaca acggcgaaat
     3121 ggaagtacag gctgctttcg gctttgttaa gaacgtccgt cgttatgcct ccatcctcca
     3181 tgcccgacct atgattcttt gggatggatt tagtgacaag cgtcgcgact tttacccgga
     3241 ctacaaagca aatcgcgacg acgatcctga tatgaaaaag atgaaggaag gctttgctat
     3301 ccagaagcca tacatcctca aaatgatgac cgcgcttgga gttacccaac tcattgcaaa
     3361 agatgcagaa gcggatgatc tggccgggct gctggtatcc cgcatggcac cgcagccaac
     3421 cgttgaacac atctatctgt taacaggcga tagcgactgg cttcagttag ttcgtgaaaa
     3481 cgtaagctgg gtaagcctgc gtgaagacgc caaaaacaag caggttaatt ttgagcaatt
     3541 tgcggagctg acaggattcg ccacgcctcg cgcatttttg gaagcaaaag cattacaagg
     3601 cgataactcg gacaacatta gcggtgttgg tggcattggt gctggcggtg cgaaagagct
     3661 gctgcatgaa tggggaagtg tcgcaacgat ggtacgcggc atcaacgacg gctcaatcgt
     3721 ggttaataaa gggcgtcata agaccgcctt caacaaacta gcgaagaatg ccttcaacga
     3781 gaaaacaggc tgtcgaatgc tcgaagcgtt caagagaaac atcacgctaa tgaacctgat
     3841 tgagacgaag tttccgccta ccgaaatcga aacaatcaaa ggcaatcgtg acgtgaaagc
     3901 attcgagcaa ctgtgctacg agctgaattt ccgttcgttc cttgaagacc ttgaagtgtt
     3961 tgttcttcca ttcgaaaggt attgctaatg cttaaatcga ttatcaatgg cgctacaacc
     4021 acccctgccc aactggcaaa agagattgtc ttttatcacg gtgagtacgc tgtcatcgca
     4081 ctgccgtcaa ttctaggcgc tgccggaatg aaagcgacag atcgcgagtt tggattagtc
     4141 agcgagcagg tcgtaaaaat cctcgctcgt gtatccagac tccttaacca cgatgcgatt
     4201 gtattcgatg aatccgccgc tttaaaacga atcaacgaaa caaaaggagc ctgatcatgg
     4261 caaaaggaaa atccgcactg gcacttgctc tgaaaaagaa aatcggtagc aacgacgaaa
     4321 ttcagaaagt aactcattgg attgatacag gctttccacc gttaaacaaa gctatttctg
     4381 gtcgttacga tggcggcttc ccatgtggtc gtatcgtcga agtattcggt cctccaagtg
     4441 cagggaaaac gttccttgca acagcagcga tgatctccgc tcaaaaacaa gatggtctgg
     4501 cggtattcct cgatcatgaa aacagttttg acgttggcct ggctgtcgcc aatggcctga
     4561 acgcagatga ggatgacggt cagtgggtat acaagcagcc agataccttc gaagactctg
     4621 tagagttgat cggcacaata cttaaattgg ttcgtgatga agagcttatc cccgaatcag
     4681 caccaatctg tatcgtggct gactctcttg cgtctatggt tccgaactcc aaagccgaga
     4741 agttcgaaaa gatggctgaa ggcactgcca aagacaaaga tcagctaaac atgaacgaca
     4801 atacggcact ggctcgtgcg acgagtgcga acttccctac tctggctttg tgggcacgca
     4861 aatacaacgc ctgcatcatc ttcttgaatc aggttcgcac aaaaatcggt gtaatgtttg
     4921 gcgaccctac tacgtcgcca ggtggagatt caccgaagtt ctacgcttct gtacgtatcc
     4981 gtcttggtgc atcggtgatg aaggatggta aagagaagat cggccaggac gtaggcgcag
     5041 aatgcatcaa aaacaaagtt gcaccaccgt atggcaaatg cacctggaaa ttctacttcg
     5101 atcctactcg tggcctcgac gttatcgaat cgctcgtcga gtacatgctg gaagaaggat
     5161 acctgccaaa gaacgccagc gggcgagttg aaattggtga caagaaatac accaaatcgc
     5221 agatcgtcga gatgtatcgg gagaagccac tggctgaaat cattgcggct ttgcaggcaa
     5281 tcgacgaccg aagagcaaaa gacaacccca ccgagtcagt agaagagtaa acacaaggcg
     5341 tccacaggac gcctttttta tctcttgaaa atatataagc acttacttat tattttcgca
     5401 taacaaccac ataggaaaac acatgatcaa aatctatcta ttggcagtag ccacaggcct
     5461 ttcagtggct ctcatctacg gtttactggt tccgtcgctg atttctacca agagtgattt
     5521 agccgtcatg tttggagtta tcgttggttt tggtgctcct gtaatcggtc ttattgctgg
     5581 tcgtaagttt atcaactcat taatcaaagc aaaggggaaa taagtaatga agaaaggttt
     5641 acttgcagtt gctctggcgg ctatttgcac aatgggtctt actggctgtg atcgcgtgga
     5701 gcctggatac gttggcatca aagtaaacaa attaggtgaa gacaaaggga ttggtgaagt
     5761 ggttggcgtt ggtcgccaat ggacaggtct taacaccgaa ctttacgtat tcccgacctt
     5821 caaacaaatg aagacctacg acgagccgtt cacattccag atgagtgacg gtactgctat
     5881 tggtcacaaa attggcgttg cgtatctggt taatcgtgac aaggtaacga cggtgttcca
     5941 gacctatcgc aaaggcgtag acgatatcac cgaatcagat ctgcgtcaga aaattgccga
     6001 ctctctaaac cgtttggcca gccgtatgac cactgactca tttatcgacg gtggtaaggc
     6061 acaattgctg gacaacgcac tgaaagatat tcagaaagag atgtctccgg ttggtattga
     6121 ggtactgagc ctgtcatggg ttggaaagcc tgattaccca aaaactgtca ttgaatctat
     6181 taacgccaaa gtaacggcta accagcgtac tctgcaacgt cagcaggaag ttgaacaacg
     6241 taaagctgag gcgaatatgc tacgtgaaca ggctaatggt gaagctgatg ctatccgtgc
     6301 tcgtgcgcaa gcagaagcag acgccattcg tctgcgcggt gaagctctgc gtcaaaaccc
     6361 gaacgttatg gaactggaag ccatcaataa atggaatggc cagttaccgc agtacatgac
     6421 tcaaggggct aacactcctt tcattacagt gaaataactc ccctaaaaag ttcaggcgtc
     6481 cagttggacg ccttttttat cgcaattatc ttattaagaa aacaatttgt ttaaaaggat
     6541 aagaaaacat gacagctatt aagaaactct acgatgccgc aaacgtggct ctggatgtta
     6601 ttgatgatga agcagcaaaa ggctttcctg aacctgattg ggcgcatcag ctacgaaacg
     6661 ctatcgccga aatgaatcca ccagatccga ccaccgacga gacagactgg cagcgattta
     6721 tccgtatgta cgctcaggaa ataggtccaa cgccaacggc agagcaagca atgctgctga
     6781 aatacttcaa agaggcggga gaggatttac caattgatga ctcagcatat tggttccact
     6841 gcgcatggcg taagtatgac gtgatattca cacaaggcat ggggagcaaa gatatggttg
     6901 tgtggcatct actccatata gacacagccg ttgacagagt tattgaacag tttttcccta
     6961 aacaagaaga ttgatcgcct attcataact aacaaaataa gtaaacacta accacaaaag
     7021 gaaaaacaca tgagagtttt agttcgaatc gttaccagca ctgtctatga cgtgtttccg
     7081 ctttttatgg tcaaagccga tggccttaac gacgaagaaa ctgacgcgct gatccagcgt
     7141 attctcgttg aatatacagg tcatgacgct gattcagtga tggttgatga tgatggtgtt
     7201 tgttggcata acggcaactg ttggtacgta gaagagactc aacaaatcag tgatgaagat
     7261 gccgcacatc ttgagcgtat tttaagcatc agcacttttg agtgagttta cagtaaaatt
     7321 tatataagtt agtatctacc tatcatgaag atttttattg aatacttgtt actcatcgtt
     7381 tcaatagctt ttgtcatcga ctgcattttc accggtgtca ttcgtaaagt cttttccccg
     7441 gtgaacgacg tagtcataaa cgctttggct atcgtgctcg tatttaattc agcatttgat
     7501 gtaatcaaag aggtggcagc atgaaggcca tcccattcgc gctgttgttc ctttcttcga
     7561 tcgttgtggc cgacaccact gtttatcagt gtgaaatgtc tgtagccgac gttaagaatg
     7621 gcgctcttac cgacgtcata aaagcaccat atggagcgat ggtcgtagac agcggcgacc
     7681 agttctatgt tgtgcgtgac gatcgagtgt tgtcatcccc atatctcaca aatcgtaatg
     7741 gcaaattaac cggcgtcgga gaagaccact tcgtatacaa caaatacaag ggcttctatg
     7801 gtgttcacgc ttctcagcaa agctaccttt tcgatgactg caaggaggtt ggataatggc
     7861 attaacactg gcaggtctgg aaatcgagaa aacaagcggc tactggcgtg ctaagggttt
     7921 caagcagcct ggcattcttg agcgtctgga acgtgaagat gggtatatcg tccaccagcg
     7981 gcgtgaatgg cgtatgtacg atccagaaac aggaaaactg actacaaaag ccggaacact
     8041 ttggggtctg ttaaagaaaa tacactaaat gcaaactgac tgcggcacgt tccgcagtca
     8101 tatttcatag tcgtcaccgc tgacagcata cacaatcaac taccgctgat agcatatcga
     8161 gagtctatct caccgctcac agcatacttt actcgatttt ttaccgctga cagcatactt
     8221 aagacattgc atgaataatg tgtaccggta tgggtataac cagaacaaaa ttaccgctgg
     8281 cagcatacga aggtctgaca tataccatta attaccgctg atagcatatc caaacaaaaa
     8341 ttcctcaata aaacaccgct gacagcatac gttctatcag ggagcagcag gcaataaatg
     8401 cctttcacta caagcaatca gcgcaatagc aatagaatgt tagtgagcgc aaacctatat
     8461 ggaatgcact cttcgaggtt agtaaccact ggggaggtat gacagagcat tgagtggtga
     8521 tagatgattt accgcccaca gcatacgttc atctcactat accgctggta gcatatattt
     8581 aaccgtttac agcatacttt tcagaaaaat agccgctgat agcatacatt tcaccgctga
     8641 cagcatatca aagcagtttg agactattgg aaaggatctc aatcatcttg atattttcag
     8701 gcgtcaaatt ctgcgaaagt tcggttatct tgttgataat gttctgtttg gcatcaattt
     8761 ccccagcttt ctcatctggt tttttgggtt cgatgtcttc aggttttggc ggtgcgactt
     8821 tgagttttgg attgcggctg tgaatctgga tatagatcga ccgccctcgc ttaatctcgc
     8881 tgtattcgag ataacccaaa tcttggagag cttttaagcc gttgcgtata gtctgattct
     8941 gcgagctgac attcctgcta ctcaaattga gtcgcgcacg caatcgagca agcgataccg
     9001 gcgcaggctt ggttggaaga ctttcgatga aggtgtacag agcctgtgct gtttctttgc
     9061 gtggtagctt attgataacc tttaactgca aaagaacctt atggtcaaag cgatatagtt
     9121 cggccagctt cggttctgca tagaacacca ccgtatcttt ctgctcgttg tagtccacgc
     9181 tattgatgag gtgcaccatc agaagcgaga tcttgttaga gccgtcgacg ttcttttctt
     9241 catacgttct ctggaaagac agagttgtac gcatgatctt caaaagactg tttgtaagcc
     9301 ggtcgcggag tgttttgcgg atctgtgacg atggatagcc acaaaacttc gcaaatttcg
     9361 tgatgcttaa ctcgacacga ccgttaggtt cgccgtattc tgccagcgaa cgcacaacgc
     9421 ccacccacgt tttgaaatca tgatccatgt cgagacgagg accggttatc ttgatatcgg
     9481 aatagccttc agaacgggct acttcgagct gaacaagctc ctttgaagca tcgatctcat
     9541 ttggcttgtt acgcttgctg tattttgtcc ccttgagcgt gggcacgaac aatcccagcc
     9601 gcatcaacgc aattggttgg actgtattgt tgctattagg gacaagttcc cctgtgtaca
     9661 attcaaggga accttcttca aagttgtcga gattatcttc tacttctttg ttatttttac
     9721 cttttttatt ttttgtggac atgtggacac ctttgtcatt caaccgctga cagcatactt
     9781 gatttgccgc tgacagaata ccaaaaacag ttggcagcat acggtgaacc gctgacagac
     9841 tatcaattac cgctgacagc atacatgaac atggcttcag accagtcgtg gcgcggctta
     9901 cagcgatcgg ggatcttatt tgatctatac aaggatctat ctatggatct ctttattagg
     9961 atctatcctg tggatatgtg aataattaaa acaggcattt actaccttcg gcgcacctgg
    10021 tgggttatcg ttgcctcggc taacaatcac agaaaaatga catatggatc taaaacgcac
    10081 gcgctgggtt cgtcgtcttg aagacggctc ctacactatc gaatcaaatt ccaacctgaa
    10141 taagcagaag ttgctttgtg acatctgcgg tatagcggcg aagtgcccga tctacgaaac
    10201 cagaattaaa cttgataagg ctggtgtgaa ttttcattta aacagttgca tcaggtacgt
    10261 tccattactc gcatttcgta aaccgatcat cggattggat gctccctact tcaacacact
    10321 ccgttcaggt gtgacgtggc gagatcgttt atcaccagac aagctgattt gcctcgtatc
    10381 cgcagacaca gggaaaatca tccgttttgg gaaagtagac aaggtttact caggcccagt
    10441 agacgaaatg ttgcggaaac acagccggtt taatcatctc tgtatgggtg gtgagaaaat
    10501 cgagaaagta gaagaagtga tccgcaaatc ctacggacac tttctgacca aagatagcct
    10561 gctcaccgca atctacatca gacatgtaaa acgtgagttc gacctcgaat accacagcga
    10621 agaagagctt aaccttgttg acccacgtcc aaaagctggc gtcataagca taaacgcagc
    10681 gcgtaaaaag cccactgacg cgctgtaacc ctccagatcg tatattggcg tagatagaat
    10741 ctacgccccc tcaaaatagc tctcatagcg ttctacagtg atcctgtctt atttttagtc
    10801 atacagacaa gcaaagttgc gccacgataa ataggtatat acttacttat aaattttgta
    10861 tattaaggcg ctcgtttcat tcctaacata ccgttatgca tagttgttta cctcctcatt
    10921 gctcttagaa tttgtatcaa aataaccaca aaggaaaaat acatgacttt gccatacggc
    10981 gtcatttctg actgccacta ccacaaatgg gatgcgttct ccacgacgaa cgctgagggg
    11041 cttaactcca gacttgaaat acagttggaa gcaacgaaag aagcagccat cgccatgaag
    11101 aaggccggtt gtaagtacat gttggttgcc ggtgatacat ttcacgtccg aggaactgtg
    11161 tccccttctg ttttgcatta cgtaactgaa acgtacaagt ggattatcaa cgagcttgat
    11221 ctgacagtag taatgctggc cggtaatcac gatcttgaaa ccaacgattc agtatatagc
    11281 gccaacgcag cagcatcgct gagttctatc ggcgtggtaa tcgtatgtgg caagcgccca
    11341 cactcaataa aaattggtga tgtgactgtc cacctgatta gctggcgtaa caatcatgcg
    11401 gagcttatca gcgatctgaa agcattacgt aagagcgtag aaggtgataa tcatgacgtt
    11461 gttatccata catccattaa caaagccatt ccaacaatgc ctgacgtcgg tatcgatgcg
    11521 caggagttaa aggatatcgg ctttcgtctc gtgcttagtg ggcattacca caaccacaaa
    11581 gaggtcattc ctggagttat cagtgtcggt gcactgactc atcaaaattg gggagatgtt
    11641 ggatctctgg ctggctacat gatcgtaaac ccggacggca gtttcagtca ctacgaaacc
    11701 agtgcgccta aattcattaa cctggaagat gatgttgctg atgaccaaat tcgcggcaac
    11761 tacgtgcgtt tccgcgccgt aattgagaac gatgaagaag gcattaagta ccagaacatc
    11821 ctcaaaacaa tgggtgcaaa aggtgtcgtg tgcaacttca tccgtaagtc atcaatgatg
    11881 gaagggacag ccagcacaac tgaaaccagc aaaatcgata gcctgggaga gtcggtatct
    11941 gcttattgca agattgtcca cgatactgac ggcggatttg atctgagcaa attggatatt
    12001 ttgtgtcagg aaatcctcac cgaagcggag agttcggagg ctgtgtgaag caaagtcgtt
    12061 atgggagctt tcgagacttt gccatcacga tgaaaagact tgaacgaggc cagacggtga
    12121 tgtttcacaa gccctacccg ccacaaggaa atcccgtagc gttttatctt ggaaggttaa
    12181 caagaaaagg cgtattgagg cgcagatcct tcccggcgca tacggagttc agattgaaag
    12241 aaggccaaaa gctaacacac ggtatcagag gtgttatatg aagtttttaa agctccaggt
    12301 tgagaatttt atggctatcg ccagcgcgga ggtcgagtta gatcagcgtg gtttagtgct
    12361 tattcagggt gttaatagtg atgatagttc cgcatcaagt aatggctctg gaaagtcaac
    12421 tctaatgaat agcctgatgt ggtgtcttta tggcgaaaca gctcatggtg tgaagggtga
    12481 cgatgtgttg tctaccgacc atgaaaagaa ctgtcgtgtt gcagtaacca tcgaggatga
    12541 aggcaagaga tacgcaatca ttcgtcaccg taaacacaaa gagttcaaaa atcgtcttat
    12601 cgttcgtggt gaagatggcg atatgacgaa aggcaaagat gcgctgacgc aggagttcgt
    12661 cgagcgtctg atcggtgcat ctaaagaggt tttcatggct tccatctatg cgagccaaga
    12721 agctatgcca gatttacctg gaatgtccga caaaaacctc aaaaccatcg tagaagaagc
    12781 cgctggcgtt gacagactga cacgcgccta cgctattgct cgtgagcgag ctaatgcagc
    12841 tgccgcacgt atggatgtgg ttaaaaccaa attggagtcg acaatctcga ccattgaggc
    12901 aacacagtca gaaattgagt cagcgaaagc ctcctctgaa tcatgggagc aagagcgttc
    12961 taaacgttat gacgatgccc tggctgggct ggccagtgcc gaagttgagt taacggaagt
    13021 tgaacttgag atccgcactc ttcccgaaca gatacgtgat accgagaagg caatcgaaag
    13081 tgagcgcaaa aagttagcct caaaagaaga acatgacgcc aagttgctca aagttcgtgg
    13141 tgcgataact gatattcggg caagcatcaa agctacagaa aatagtcagg ctgatgcaat
    13201 gagccgcgcg cgcaatttta agaccaaagc agaagaggtt ggtactaaag taggatcacc
    13261 atgccctact tgtggcaaag cctactgcga agaagatcta tcaacggtga aggagaattt
    13321 cattgaacaa gcacgtcagg aaattggtca ggcgaagaca cttgcagagg caatggctaa
    13381 acacaaaacg aatcttgaga aagcgttaag cattgagtct gcccttgtta aaacgacacc
    13441 tgatgtaacg gctatcattg cccggattga agagcttacg aaacaactct catctttgcg
    13501 tcatcgtgag aaggaggttg ttgctattga gtctcttgtg actcgtgctc gtactgaggt
    13561 cgatcgtata tcaaaagaga ttaatccgtt tattgctctt atcgccagac acgaagataa
    13621 cttggtatcc agtaagtcta ccttcaagtc cttaaaagat gagttgaagg ctattcagga
    13681 acaaacgttg ctattggaaa aagctcgtca ggtctactct cctgccgggg tgcgttctca
    13741 tattttgacg tctgttacgc ctttcctgaa tacacgcact gccgagtatc tcaatacgtt
    13801 gtctgacggg aatattactg ctgagtggtc gacgatggat gtcactaaaa aaggtgagta
    13861 tcgcgacaaa ttcaacatta gtgtgcagaa gaaaggttca agtaagtcgt tccagaccct
    13921 ctctggtggt gagaagcgga aggttcgcat tgcgtgttct ttggcattgc aggatctggt
    13981 tagtaaccgg gcgagtaaaa acatcgattt gtttatcggc gacgaaattg acgatgcact
    14041 cgatacagcc ggtcttgaac gcctcatggg tattctggag tccaaagctc gcgagcgagg
    14101 tactgtgctg attatctccc ataaagagat gaagtcatgg ttccgggaaa ctattacgct
    14161 ggaagttaaa gaggggcgca gctatgtcgt ttaaattaag ccgctcgcag tttttgcagg
    14221 tatttgcagt gatgcagtcg ataaaactga tcaatgggca tacttccaat ggtgcggctc
    14281 cacgtattct gtggggcagc aacaatattg acggagtaca attcgccgcg ttgcttggtc
    14341 taatatccga gacgccattg atgcaaagtt tgaaatcact accacctgga tgtattgcgc
    14401 cgatcctgat taatcctttt gttgaggggg gatatcttcc caacgtcggg cctgggttta
    14461 ttgcatccca tgaaactgaa gatcttaaca ttaatagcga aggggtcttt gggggaatgg
    14521 gtgcgcatca ctgtatggct ttcacgaacc ttattcgact tgccaataag cgggtggata
    14581 gtttggcatc gccaggtgat gcttttactg gtttccttat ccaaaggagg gataaaaagt
    14641 acagtgcgga caaactacag tttgttggta agtatggaga aatggtagaa atcgaacttc
    14701 agctccctca tgttttagca aacgatagtg cagacagtcg gaggctattg ggcatcatgc
    14761 gtcatttcat agcaagtggt gttaaacatg ccgcagataa acgtgtcacg caggaaaatg
    14821 agtattcaga ctttgcaaac tatccccaac caacgttgca aacggcaata gtagccaatt
    14881 cgttggaggc gagattattg gaaaacccta tatggggaac atggtaagga gactatatga
    14941 gtaaaaaaat cagcgtagtt ggtgttgatc cctcaatgag caactttggg cttgctgtgg
    15001 gcactttaga ccttgagacg gacgaacttg agattcacgg ccttactctt gttgagacta
    15061 aagcggggag taacaaaaag accgttcgtg tgaacagtga cgatctgcgc cgcgccagtg
    15121 aaatatggcg tgttgcgaag ccaatcattg ataaggcaaa tatggttttt tgtgagctac
    15181 cggttgggag ccaaaactct cgttcgcaga cgtcttacgg tatttgtatc ggtgtacttg
    15241 cgtgtgtgga taagccattg atccaggtta ctccaaacga aatcaagcat tttgtcggca
    15301 ataaacttac tacatcgaaa gaagagatta tccagtgggc tacgaaaaaa caccctaaag
    15361 caccgtggct gcgtcgtaag caatctggac aggatgttct cgtgaacaaa aacgaacatt
    15421 tggctgatgc ggtggctgcc atccataccg gtatgcaaac agatcagttc cgccaggtgc
    15481 gcgatgttct taagtctctc atttgatttc attgataggt aagtgcttat ctattaacat
    15541 gggccactat atttagtggc cctctttatt tggtgataca tgataagcat cgtaaaacgt
    15601 aacggccaaa cagagccgtt atccgaagaa aaatacaacc gcgtcgtaat gtatggcgta
    15661 gaaggcattc gtggtgtaag cgcatccgct gtagcaatgg gagctgcggc cagcattttt
    15721 gatgggatta ccaccagcca gttgcatgag gctttggtta aatctgccgc tgatttgatc
    15781 tcaccagaag caccaaatta ctcacaggtg gctgcccgcc tgaacatttt taaaatccgc
    15841 aaagatgcct tcggtcgtta cgactatccg aacttctacc aacacattgt caagaacgtt
    15901 aacaagggcg tttatgacaa ggatttgctg acacattatt cgtttgaaga gatcgaagaa
    15961 ctcggcaatt acattaagcc gaaacgtgac gatctttttg gctatgcagc tacggtgcag
    16021 ttgcaaagca aatacctcgt tcaaaaccgt gttactggtg agattcatga ggggccgcaa
    16081 catatctata tgctggtagg catgtgtctg ttccagaatt gggaagacga ctgcgctggc
    16141 aaaacacgta tggagatggt caaaggtttc tatgacgtta caagtacgtt caaactgtct
    16201 ctgcccacac caatcatggc cggcgtccgt actccaaccc gtcagttctc cagttgtgtg
    16261 ctgattgagt ctggcgatag tctgaaaggg attagtgcag cttcagccgc aattatcgac
    16321 tacgtttcac gtcgtgctgg aattggtatt ggttttggcc gtatccgtgc gctgggcagc
    16381 gagatccgca atggtgaagc cacccatacc ggagttattc cattcctgaa gcatttccag
    16441 acggctgtta aatcttgttc gcaaggtggt gttcgtggtg gcgcagcaac agcgttttac
    16501 ccgatctggc atcttgaagt tgaaagtctg ctggtggtga aaaataaccg tggtattgat
    16561 gaaaaccgcg ttcgccatct tgattacggc gtcatgagta accgtctaat gtaccgtcga
    16621 ctcgtcagaa gcgagaacat cactctgttc agcccgcatg atgtgcctga tatgtacgaa
    16681 gccttcttca cagaccagga gctgtttgaa aagctgtacc ataaatacga agccgatgat
    16741 tcaattcgca agaagccagt acctgccatt gagctgttct catctctgat gcaggaacga
    16801 gcgtccacgg gccgaattta tattgcgaac gtagatcata ttaacgagca tggcgctttc
    16861 attcctgctc ttgcacctgt tcgccagtca aacctgtgca tggagatcac tctacccact
    16921 cgtccactgg catttaccga cgacccgaac ggtgagatcg cgctatgcac tttatccgct
    16981 tttaacctcg gagccatccg ttcactggag tctcttaaag aggtggcgtt ctatgccgtt
    17041 gctgcactgg attcgttact ggattatcaa gactatccga tggaggcagc cgaagtgcct
    17101 gccaaagctc gtcgtagctt gggaattggt gtaaccaact ttgcttatta cctggcaaag
    17161 aatggcgttc gttattctga taccgctggc aataaactgg tgcatgaaac gttcgaagct
    17221 atccagtatt accttcttga tgccagctgc cgacttgctg aagcaaaagg tgagtgtgac
    17281 tggtttgagc agaccaagta cgcaattggt cagttgccga tcgaccatta ccgttcttca
    17341 ttagacgaaa gtggcgaaac caactttgag ttaaagatgc cgtgggaaga actgcgtgaa
    17401 cgtattgcaa aatacggcct tcgcaactcc acactgacgg cacaaatgcc atgcgagact
    17461 tccagccaga tcactaattc caccaacggc atcgaaccgc ctcgtggccc ggtgtcggtg
    17521 aaatcttcta aggacggcat cgttaagatg gtcgtgcctg agtttgaaaa actgaaggaa
    17581 cagtatgaat acctgtggga tatgccggac aaccgcggct atctgacaaa ggtggcgatc
    17641 atccagaagt tctttgacca ggctatttca gccaatacca actatgaccc ttctcgcttt
    17701 gaaggcgata aagtcccaat gatgacgcta ctgtcagatt tgcttctcgc ctacaagatg
    17761 ggagttaaaa cgctttacta ccacaacacc agagatgggg caggaaagcg tgatgacgac
    17821 gaaccgcaga atccactgac gcaagctgta gccgtcgagc cagaagatga gtgcgacgga
    17881 gcctgcaaaa tctgacatat ggtgggggat atccccacct tctctttgat ttgtaagcct
    17941 tgtttaaaca cataagataa caacttgttt aaacgcacca aaaaagaaaa aggaaaaaca
    18001 catgtcatat tcaacgttcc gtttgggtgc taatgatgca accaaagagc ctatgttcct
    18061 cggacaatct gtcaacgtgg cacgttacga tcagcaaaaa taccgtgatt ttgaaaagtt
    18121 gattgaacgt caattgtctt tcttctggcg gccggaagaa gttgatattt cgagcgatcg
    18181 tatcgacttc aacacgaagc tgcgggacca cgaacgtcac atttttctga gcaatctccg
    18241 ttatcaaacg ttactcgatt cagttcaggg acgtagccca aatgcaacgc tgctgccgct
    18301 tatctctatt cctgaactgg aaacgtgggt tgaaacatgg tctttctctg agactatcca
    18361 tagccgcagc tacacccaca ttattcgtgg catggtggac gatccgagca ttgtttttga
    18421 cggtattgtt acggatgaag aaatcatcaa ccgagcgatc agtatctctg ctgaatatga
    18481 caggctttat gggatgacct gcgagcgcca gtcgttaggt gagaaagagt ttgaacgtct
    18541 gtacgtaaat gaatatggct gggagccata ccctttgcat cgtcagcttt tccgcacgtt
    18601 ggtgtccatt aatgcgcttg aggcgatccg tttctatgta agttttgcat gtacgtttgc
    18661 ctttggcgaa cggaagttgc ttgagggtaa caccaaaatt atgcgcttta ttgcccgtga
    18721 tgaagctctg cattgcgaag gaactgaacg catgatccgc ttcatgcgta ccggtcgcga
    18781 aggtttattg tggaaagaga ttgctgctga tgaagaaaac gtcatttacg acaccatgaa
    18841 atcagtcgcc gaacaagaaa tgaactgggc agactatctc ttcaaagacg gttcgatgat
    18901 tggtttaaac gcggatattc tgaagaccta tgtaaaatac cgaaccaatc tggctatgaa
    18961 tcgtcttggc ctgaaggctt tatttccaga agttaccact gatccgctgg tctggatgaa
    19021 caagtggttg ttaaccgaca cactgcaaat tgcaccacaa gaggcagagc aaagcacata
    19081 tctggtaggt cagatcgatt ctaccgtgga taaggcttct ctaagccagt ttgcagacct
    19141 gtaaaccgat acaaagcatt atgtggcctg gcaacgctgg gccacaatgg atcacaagaa
    19201 ttaagaagga acaaaactag catgaacttt accaaactga ctgaccacct gaaacttgcc
    19261 accgatcgac tcattggatt taagccagaa ccatatgagt tgcatgaagg ccatggtgta
    19321 gccactgaaa gtatttacaa gatggtcgat cagtttcatg aactcttcca gcatccgaga
    19381 cgcgttatgc cgacaccaga gctgcttcgt ctccgtgcaa gcctgattca tgaagaagct
    19441 gtagtggaag gtattccagc tgcaatgaat ggggatattg agcaactgct ggatgcaatg
    19501 gccgactttt tatacgttgg tgttggtacg atggtcgcca tcaaaggtgg tatttctacc
    19561 ggcatgacct attacacgca ggaacagagc attgatcgct ttatgcagac aatttttgtg
    19621 cccggtaaca ctgttttcga tgatatggca atgccatttc aggaagctcg tgaggcgtca
    19681 tgtatgctcg aagagctggc agataaactt gagaacaaga ctgttaagga ttctgagctg
    19741 attcaggaac tgcgccgtgt catgaacaaa atctatgtgg cgtgcatgat gacctatcga
    19801 ctggctgatt tcctcggtat caatgtagtc gagctggttg gcgaaattca tcggtctaac
    19861 atgacaaaat tatggcctgc tgatgtagag gaacgtcgcc aggctgtggc caactgcaaa
    19921 tacgactctt cagacctggg atttcgccat gctgatggta ccgataagat gatcggtttt
    19981 cgaatttccg atggaaagat tctgaagtct ccaacctata gtgatgtcga tttatcctcc
    20041 tttgttgagc aagctaaagc ctcagcaatg tacgg
//
LOCUS       DBANUU010000084        19894 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000084 DBANUU010000000
VERSION     DBANUU010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.084"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            239..739
                     /gene="tssB"
                     /locus_tag="ACIE68_003024"
     CDS             239..739
                     /gene="tssB"
                     /locus_tag="ACIE68_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFX5909305.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            774..998
                     /locus_tag="ACIE68_003025"
     CDS             774..998
                     /locus_tag="ACIE68_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909306.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1049..2524
                     /gene="tssC"
                     /locus_tag="ACIE68_003026"
     CDS             1049..2524
                     /gene="tssC"
                     /locus_tag="ACIE68_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFX5909307.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2531..2944
                     /gene="tssE"
                     /locus_tag="ACIE68_003027"
     CDS             2531..2944
                     /gene="tssE"
                     /locus_tag="ACIE68_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFX5909308.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGNQHYRVD"
     gene            2948..4798
                     /gene="tssF"
                     /locus_tag="ACIE68_003028"
     CDS             2948..4798
                     /gene="tssF"
                     /locus_tag="ACIE68_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFX5909309.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4762..5844
                     /gene="tssG"
                     /locus_tag="ACIE68_003029"
     CDS             4762..5844
                     /gene="tssG"
                     /locus_tag="ACIE68_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFX5909310.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5869..7149
                     /gene="tagH"
                     /locus_tag="ACIE68_003030"
     CDS             5869..7149
                     /gene="tagH"
                     /locus_tag="ACIE68_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFX5909311.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7146..7670
                     /gene="tssJ"
                     /locus_tag="ACIE68_003031"
     CDS             7146..7670
                     /gene="tssJ"
                     /locus_tag="ACIE68_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFX5909312.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIERVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7673..9004
                     /gene="tssK"
                     /locus_tag="ACIE68_003032"
     CDS             7673..9004
                     /gene="tssK"
                     /locus_tag="ACIE68_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFX5909313.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRTGRYRLNYADVRDLHSEEGDTRTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANVPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9009..9770
                     /gene="icmH"
                     /locus_tag="ACIE68_003033"
     CDS             9009..9770
                     /gene="icmH"
                     /locus_tag="ACIE68_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFX5909314.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9779..12544
                     /gene="tssH"
                     /locus_tag="ACIE68_003034"
                     /gene_synonym="clpV"
     CDS             9779..12544
                     /gene="tssH"
                     /locus_tag="ACIE68_003034"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFX5909315.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESEQDNTGAVPADEADREQPEETAETVSPVQRLAQLTAELD
                     ALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWLGDTIKGQDLA
                     IASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINM
                     SEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQ
                     AFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFKPA
                     LLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIMSRVTRA
                     ENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDAQNDESV
                     TKDETVL"
     gene            12541..13284
                     /gene="vasI"
                     /locus_tag="ACIE68_003035"
     CDS             12541..13284
                     /gene="vasI"
                     /locus_tag="ACIE68_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFX5909316.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKHRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13289..14701
                     /gene="tssA"
                     /locus_tag="ACIE68_003036"
     CDS             13289..14701
                     /gene="tssA"
                     /locus_tag="ACIE68_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFX5909317.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14810..18244
                     /gene="tssM"
                     /locus_tag="ACIE68_003037"
     CDS             14810..18244
                     /gene="tssM"
                     /locus_tag="ACIE68_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFX5909318.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALMWLTVRVMKRMQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNQ
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18255..19704
                     /locus_tag="ACIE68_003038"
                     /pseudo
     CDS             18255..19704
                     /locus_tag="ACIE68_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19631..>19894
                     /gene="tssD"
                     /locus_tag="ACIE68_003039"
     CDS             19631..>19894
                     /gene="tssD"
                     /locus_tag="ACIE68_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFX5909319.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFI"
BASE COUNT         4664 a         5472 c         5522 g         4236 t
ORIGIN      
        1 cataatacac ctgacgcgaa ttgcacaggt tcatacaatc tatttaacgc ttgcagaata
       61 cccctcttta cgatcgataa aaagtccttt tcttatttat tttgaagcgt aaaatgcggg
      121 gtgatgaagg agtggtgatt ttattttcac cgccatcaag gcggcatttc cgatattttt
      181 tgcggtacga atatgccgtg ttcatggcct gtacgagata acatgacgga tcacttttat
      241 gagcaaaaaa tttgaaggca gcgtcgcgcc tcgcgagcgt attaatatat cgtatgtccc
      301 aaaaacagat ggccagacgg ctgaagtgga acttccgctt aacatgctcg ttgtcggcga
      361 taccggaaat acgcaggaaa catcctcact ggatgaacgt caggcagttt ccgttaacaa
      421 gcacaatttc ggtgcagtga tggctgaagc agccattggc ctcaatttta ctgtccccgc
      481 caccctgaag ggaagcacaa cagacgatga actgaatgtg gcgctgaata tcaaatcact
      541 ggatgatttt tcaccagata gcgtggcccg gcaggttccg gaagtgaata aattgctgga
      601 actgcgtgaa gcactgactg cactgaaggg gccgatgggt aaccttccgg cattccgtac
      661 ccagctccag gcattgctgg aaaatgaaga gtcccgcgag cagttgctca aagaaatcgg
      721 tctggtgagc aataaataac caccactatt tcattaaaca gggttatcca ggcatgacgg
      781 cacacaaaaa tatctccggg acataccacc tctctgttgc agacattctg caggtggttt
      841 accaggtatg tttcagcccg tccgttgaaa tcaaccagga tggtgtggct gcgctgatta
      901 caacactgga tcgccgtatc agtgatctat tggatgagat tatccatttt tgtgagtttc
      961 agcagagcgc gtcgcactgg cagcgcgtac ttcactgacg gcgaaaagcc ggtttcagaa
     1021 acacagctaa aacagaagga ttttcagtat gtctttacag gaagaggaac tcgtatccag
     1081 tcatgccggg cagcctgagc aggcatcgtc gctgcttgat caaatcatgg cacagacccg
     1141 tattcagccc ggttcggaag gttatgacgt tgcccgccag ggtgtgacag cctttattgc
     1201 cagcatcctg caatccactg catctgccga accggtcaat aaactggccg tggacagcat
     1261 gattgcagat attgatgaac gcatcagccg ccagatggat gtcatcatcc atgcgcctgc
     1321 ctttcagcag gtcgagtcct tctggcgctc actgaaaacc atggtggatc gcgttgattt
     1381 ccgtgaaaac atcaaggtca acgtgcttca tgtcaccaag caggaacttc tggaagactt
     1441 tgagtttgcg ccagaaatta ttcagtccgg attttataag cacgtttatt cgtccggttt
     1501 cggacagttt ggcggtgaac ccatcgctgc cgtgctgggc gcttatgagt tcaaaaatac
     1561 cgcgcctgac atgaaactgc tgcaatacgt cagtgccgtg ggcgcaatgg cgcacgcacc
     1621 gttcctgtcc tccgtttccc cggagtttat ggggctgaac tcgtggaccg aactgcctaa
     1681 tatcaaagat ctttatgcca tctttgaagg tccggcctac accaaatggc gcgccctgcg
     1741 tgactcggaa gattcgcgct atttagggct tacggcaccc cgcttcctgc ttcgccagcc
     1801 ctattcacca acagataacc ctgttaagaa ctttaactat tacgaagatg tcagccagaa
     1861 ccacgaagat tatctgtggg gtaatacggc ctggatgctg gcatgcaata tcgcagacag
     1921 ttttgccaaa taccgctggt gtccaaacat tatcggtccg caaagcggcg gcgcagtgaa
     1981 agatctgccg gtgcatctgt tcgaaacgat ggggcagatt caggccaaaa tcccaaccga
     2041 ggtgctggtc accgatcgcc gcgaatttga actggctgaa gagggtttta tcacccttac
     2101 catgcgtaaa gactctgata atgcagcctt tttctctgca aactcggtac aaaaaccgaa
     2161 gcactttccg ggaaaagatg cagaaaccaa ctataagctg ggtacgcagc ttccttatct
     2221 cttcatcatc aatcggttag cgcactacat caaggtgttg cagcgtgaac aactggggtc
     2281 atggaaagaa aggagcgact tagaaagaga gcttaatacc tggatccgtc agtatgttgc
     2341 cgaccaggaa aacccgcctg cagacgtgcg cagccgcaaa cctctgcgcg ctgccaaagt
     2401 tgaggttatg gatgtagaag gcgaacccgg ctggtaccag gttgcgctaa gcgtgagacc
     2461 tcattttaaa tttatggggg caaattttga gctttccctg gttggccggt tagacaggga
     2521 gtaacacacc atgataccgg atcgggaaag aaccacaggt tctctttttg agcgtatgga
     2581 agcctcttcg gccagaaacc ggcagggagg cagtattcat tcgctcaggc aatccattcg
     2641 ccagaaccta cgaaacattc ttaatacccg gtccggtagt tgccggggtg cgcctgagct
     2701 gggcattgat gaaccggaag gggctgaaaa cttccgcgaa tccatgagtc gggccatcga
     2761 gcaatgtata gaacgctatg aaccccggat ctcacacgct gaagtgcagg cagtggtttc
     2821 atccgcctcc agcccgctgg atatgacgtt tcatattaca gcctgggtga catttaacga
     2881 aacgcatgag gtgcttgaat ttgatatggc accgaatggc aaccagcatt accgggtgga
     2941 ctgacaaatg gaatttgaag aacgctattt ccgggaagaa ctcgattacc tgcgccagct
     3001 tagcaagctg ctggcaacgg aaaaacccca tctggcccgc ttcctggccg aaaaagatgc
     3061 ggatccggat attgaacgcc tgctggaagg ggtggctttt cttaccggca atctccgcca
     3121 gaaaattgag gatgaattcc cagaactgac gcacgggctt attaagatgc tatggcctaa
     3181 ttacctgcgt ccggttccgg caatgaccct tattgaatat acgccggata tggataagtc
     3241 ttctgtaccg gtgttaatcc cccgtaatga gcagtttaca accaacgccg gggaaatcag
     3301 agttgatgaa gtgctgccct ctgatgctaa aaaggaggag ccgcctccct gtaccttcac
     3361 cctctgccgg gatatctggc tgctgcccgt tcgcctggag cagattgaaa accgcagtac
     3421 gacccgtaat ggtgttatca acatcacctt ttcggtcgca ccgggaacgg acttccgcac
     3481 gctggatctg aacaaacttc gcttctggct cggcaatgac gacaactata cccgtgacca
     3541 gctttattta tggttctgcg aatacttgca gggtgccgac ctgactgtgg gtgaacagca
     3601 tattcgcctg cctgagttta tgctaaaagc tgtcggtttt gagccgcagg atgccatgct
     3661 gccctggccg aaaaacgtcc acagcggcta ccggatcctt caggagtatt tctgttaccc
     3721 cgatgcgttt ctcttttttg atctttgtgg ttgtccggct ttgcctgacg gattgcaggc
     3781 ggaattcttt accctgcaac tgcgtttttc gcgccctttg cccgtggaca tccggctgcg
     3841 ccgcgattcc ctgcgcctgt attgcgcacc tgccattaat ttatttatcc accatgcaga
     3901 agccatcacg ctggacaacc ggcgggcaga ctatccgctg gttcccagcc gccattaccc
     3961 acaacattac gatgtatttt ccgttaacag tgtggtgagc caggtccagg atatgttcag
     4021 gaaaaaagat ctggggcgtc ctgtttcgac gcaggccgcg cgccagtggc cagcctttga
     4081 aagtttcagc catcagatgg aatacagccg gaagcgggaa gtggtgtact ggcatcaccg
     4141 gaccaaaaca tccctgttcc atcgcggctt tgatcatacc cttgccttta tacatgctga
     4201 tggcagttat ccgtcagacg aatctctgct cagtaatgaa gtggtttcgg tatcgctgac
     4261 ctgtaccaac cgtgagcttc cgtcacaaat tcgttccggc gatatcaccg gcacaaccgg
     4321 taaaaatgcg gctgttgctt catttcgcaa cattacccgc ccgacgcaac cactctggcc
     4381 ggtcattgat ggcagcctgc actggtccct actctccgcc atgaacctga attatctgtc
     4441 attactggat acggacgcgc tgaagcaggt catcgccaac tttgatcgcc acgcaatcca
     4501 tcatccgcag acggcacggc tgtcacaaca aaagctggat gccattgagc gtctggagac
     4561 ccgccccgtt gatcgcctgt ttacgggtat tcccgtccgg ggactggcct ccacgctgta
     4621 tctgcacccg gagccgtttg tctgtgaagg ggaaatgtat ctgctcggta cggtgctttc
     4681 gcattttctg tcgctgtacg ccagcgttaa ctcattccac atgctgaccg ttgtgaacac
     4741 agaaagccag gagacatgga aatggacgga aagaatcggg cagcatcctc ttatctgagt
     4801 ccgggaaatc ccccggcaga taaagagcag aatgatccgc tggcacaggt tttccataat
     4861 gcctgctctt acaatttttt tgcgatggcg gagctgttgc accgcctggc aaagggtgaa
     4921 aaggggacgc cagaattatc cctgcgtgac gatccggcac aggaaaccct gcgtttcagc
     4981 gccgatgcca gccttgcctt cccgtgcagt gatatcagtg cgctgaaacg ggatacgtca
     5041 ggggcattca ggatgaccac cacctttatg gggttacagg ggagccagtc gcctctcccc
     5101 ggttattacc tcgatcacct ggcctggaaa gcggtacatg aacaaagtcc ggtaggtgat
     5161 tttctggata tgttcagcca ccgcctgacc cagtttgtct ggcatatctg gcgtaagtac
     5221 cgctatcaca tcagttttcg taacggcggt gtggatgcgt tctcgcagcg catgtactcc
     5281 cttgtcgggc tgggccaccg ccagcttcgc gataagctgg cgattaatca cagcaaaatg
     5341 ctggcgtatt ccgggatcct ggcaaatccg ggacgatccc cggaaataat ctgtggcctg
     5401 gtgtcccact gctttgattt gtcagaagtg acgctgcaaa actggcaacg tcgcaaggtg
     5461 gatattgaac cggatcagca aaatagtctg gggagctatt ctcttaaaaa tggcgaaaaa
     5521 ctggcgggaa gatcggtact gggcaacttt gttctgggta cacgagtgcc cgatctcagc
     5581 ggaaaattcc agctcagtat taccagcctg acccgaaaac agttcctctc ttttctgccg
     5641 tccggcgaaa acttcctgcc actgacgatg ttcgtgtcct tcatcctgcg cgatcagctt
     5701 gcctgggact tacatctggg gctggcgccg gagcaggtgg gcgcaatgcg cctgggcgat
     5761 aacaaaagtg cgctgctggg ctggaccagc ttcctcggca caccggaaga acgaccctca
     5821 gtcaccatca gggtacggtc gtaaattatg ataatcacgg agcagaatat gccggaagag
     5881 aaactacaga cgctttcatt gcaggtcatt aacggcagtg agctggaaag cggacgggcg
     5941 gcgcgctgtc tgttcacaca gcagggaaat gtcggccatg gccccgaatg ccactggtcg
     6001 gtacaggatc gtcagcagag cattccggcc caggctttta ccgttatcct gcacgatggc
     6061 acattttgtc tgcgcccgca gacggcacaa ctgtggctga atcaggcaaa agtcacagca
     6121 acatcagacc taatacagtt gcgccagggc gatgagatcc agatcggacg gctgatggtg
     6181 agggttcatc tgaaccgggg agatattccc cattacgatg aggaaatggc cactcccgaa
     6241 accatcgtta ccaatcgcga tatgctcacg gataccctgc tatcaacgga gggtgcgcca
     6301 cactatccgg gaatgactca ccggcaccag cttgcagaca ccgtggtaaa cggtttttct
     6361 gccgatccac tccaggcact tcagtccgaa agcctgatta ccacgggcga tccgctttca
     6421 ggcattgcgg ctgtccggcc atcggcaccg ctgtccgatc cggcaagtaa tggggggatc
     6481 aatactccgt ttatggatct gccgcccatt tatgccagcc ccggcgatcg taatgacgac
     6541 gtctctgcgg cagaaatggc gcaacgccac cttgcggtca cccccttact tcgcggtctt
     6601 ggcggctcgc ttaccatgag caattccgac gatgcggatg attttctgga ggaggccgga
     6661 cgaacgttac aggccgcaat aaaaggtctg ctcgatttgc agcagcagcg taacagcctc
     6721 tcagacaaac atttgcgccc gctggaagat aacccgctgc gcctgaacat ggattacgcc
     6781 accgcgctcg acgtaatgtt tgccgaaggt aaaagcccgg tacatctggc ggctcccgcc
     6841 gccgtcagtg aaagcctgcg caatgtccgc caccacgaag aagctaaccg ggcagcaatt
     6901 gtggagtcgc ttcgtgtcct gctggatgct ttctcaccac aaaatctgct gcgccgcttt
     6961 gtgcagtacc gccgcagcca tgaactgcgc cagccgctgg atgatgccgg agcatggcaa
     7021 atgtacagcc attattacga agaactggcc tccgatcgcc agcaggggtt tgagatgctg
     7081 tttaacgagg tctacgccca ggtctatgac cgggtgcttc gtgaaaaaca gcgggagccg
     7141 gaagcatgaa caggaaggcg tttctggcct gcgtgctgat gtgcgtcata ttaaccggct
     7201 gcgaaacggc gaaaaaaatc agcgaggtga tcaaaaaccc ggatatccag gtcgggagcc
     7261 tgaaggagca accttcggaa ataaccgtca ccctgctgac cgaaccggac accaatacca
     7321 atgcggaggg tgaatcagcc gccgtggatg tgcaactggt gtacctgact gacgactcaa
     7381 aattgctggc agccgactat gaccagatag ccagtacgcc gctaccggat gtgttgggta
     7441 aaaactatat cgaccaccag gatttcaacc tgttgccgga caccatcaaa accctgccgc
     7501 cggtaaagct ggatgagaaa acgcagttta ttggtgtcgt ggcctatttc tccgatgacc
     7561 aggccactga atggaaacaa atcgagaggg tggaaggaac cggccaccat taccgcctgc
     7621 tggtgcatgt acgtcaaagc agcatcgaaa tgaaaaaaga ggatgaataa ttatggcaac
     7681 cacccgcaac aaggtgatgt ggcaggaagg gatgctgatg cgcccacacc atttccagca
     7741 acagcagcgt tacaacgact acctggataa ccagcgtttc cgggccatga atgatttatc
     7801 ctggggattt accgaactca ccctcaacaa tgaactgctg gcgcagggta agatcatgat
     7861 tgacagcgcg tcaggcacac tgcccgacgg caccgtcttt tctatccccg accaggacgc
     7921 actgcccgat ccgctgcacc cgcaaaattt tccggacgaa agaagccgca atatctacct
     7981 cgctctgccg gtcgccagtg atgtgagaaa tgaaatcagc gacggacggc gaaccgggcg
     8041 ttaccggctg aattatgccg atgtccggga tttgcattca gaagaaggtg acacgcgaac
     8101 attgacgctg ggtcaactga cgccgcgcat tatgagcggt gcagaagata tgagcgccta
     8161 tattacgctc ccgctttgcc gtatcagcga tcgccatgcc gatggctccc tgacgttgga
     8221 tgacgatttt atcccctcct gccagaatat tcaggtcagt aagaaactgc gtgtttatct
     8281 caaagaggta cagggggcca ttggcggacg ggcaagcgat ctggcaaacc gtattggctc
     8341 tccggcgcag agcggcatcg cggatgtggc ggaatttatg atgttgcagt tacttaaccg
     8401 taaccagacc cggtttaccc atcgcgcccg ccgatcccag ctccacccgg aagatttcta
     8461 ccttgatctt gctgggttgc tgggtgaact gatgaccttt accgagccgt cgcgcctgcc
     8521 ctgcccgctt gatgtgtatg atcatcatga tctgaccaaa acatttaaaa cactgttacc
     8581 ggaagtcaaa cgggcgctgc ataccgtact gtcgccaaga gcggtcaatc tgccgctgca
     8641 tctgcgtgac ggtatctggc aggccgatat ccatgacaca gaactgctgc aatctgccac
     8701 ctttgtgctg gccgtggcgg caaacgtgcc ggtcgatcag atccagcgtc agtttatcca
     8761 gcagtcgaaa atttcctcgc cggaaaaaat ccgcaatatg gtcagtgtgc agatccccgg
     8821 tattccattg cgtgccctta tggtggctcc ccgccagctt ccttaccatt ccgggttcag
     8881 ctatttcgaa ctcgacaaga gtggccaggc ctggacagaa atggctgccg ccggggccgt
     8941 tgcactgcat gtttccggca gtttcccgga tctgaacatg caactgtggg cgataagagg
     9001 gtaagacgat ggatgagggg tcactgtctc tgccaccatt taccggacac gacgaaaaaa
     9061 gccagcgcaa ctaccatctc gcgcttcgtg gcaacagcct gaatccgatg atcgacgcgg
     9121 ccacgccgct actcggtatg gtaatgcgcc tttcaaccat gaacagccag acgatgccgg
     9181 agcatctttt tgctcaggtg gtgacagatg ttcaggccgt ggagcaactg cttcaggagc
     9241 agggctacga accgggcgtg attatctcgt ttcgctacat cctctgcacg tttattgatg
     9301 aagcggcgct cggtaacggc tggtcaaaca agaacgagtg gatcaagcag tcattgctgg
     9361 tccatttcca caacgaagcc tggggtggcg agaaagtttt catcctgctg gagcgcctga
     9421 tccgtgaacc caaacgttac caggatctgc tggaatttct ctggatctgt ttttctctgg
     9481 gatttcgcgg gcgctacaaa gtggcagcac aggatcaggg ggaatttgag cagatctacc
     9541 gccgtctgta ccacgttctg cataaactgc gcggcgatgc ccctttcccg ctgttacacc
     9601 aggacaaaaa aacccagggc gggcgttatc agctcatcag tcgcctgact gttaaacata
     9661 ttttttgtgg tggcgttgtc gtgctggcgt tgttttacct gttctatctg ttgcggctgg
     9721 acagccagac gcaggacatc ctgcaccagc taaacaaatt acttcgataa ggacgtttat
     9781 gatccagatt gatcttccca cgctggtaaa acggctgaac ctgttctccc gccaggcgct
     9841 ggagatggcc gcctctgaat gtatgagtca gcaggcagcg gaaattaccg tcagccatgt
     9901 gctcattcag atgctcgcca tgccacgcag tgacctgcgg gttattaccc gccagggcga
     9961 tattggcatg gaagagttgc gccaggcgct gacggtagag aactacacaa ccgcccgttc
    10021 tgcggacagc tacccggcgt tttccccgat gctggttgag tggcttaaag agggctggct
    10081 gctggcgtcg gctgagatgc agcacagcga actgcgcggc ggcgtgttgc tgctggccct
    10141 gctgcattcg ccgctgcgtt atataccgcc tgctgccgcc cggctgttga ccggcattaa
    10201 ccgtgaccgt ctgcaacagg actttgtgca gtggacacag gagtcggcgg aatcagtcgt
    10261 gccggatgca gacggtaaag gcgcaggcac aatgacggac gcctctgaca ccctgcttgc
    10321 ccgctatgcc aaaaacatga ccgcagacgc ccgtaacggc aggcttgacc cggtactgtg
    10381 ccgcgaccac gaaatcgacc tgatgatcga cattctctgc cgccgccgta aaaacaaccc
    10441 ggtggtggtg ggcgaagcgg gcgtgggcaa aagcgcactg attgaagggc tggcgctgcg
    10501 catcgtggca ggccaggtgc cggacaagct gaaaaacacc gatatcatga cccttgactt
    10561 gggcgcattg caggccgggg cgtcggtgaa gggtgaattc gaaaaacgtt tcaaagggct
    10621 gatggcggag gtcatttcct ccccggtgcc ggtcattctg tttatcgacg aagcacatac
    10681 cctgattggc gcgggcaacc agcagggcgg gctggatatc tccaacctgc tcaaaccggc
    10741 gctggcgcgc ggcgagctga aaaccatcgc cgccaccacc tggagcgagt acaaaaaata
    10801 cttcgaaaaa gatgccgccc tgtcgcgccg cttccagttg gtgaaggtca gcgaacccaa
    10861 cgctgccgaa gccaccatta ttctgcgcgg tctgtcggcg gtctatgaac agtctcacgg
    10921 cgtgctgatt gatgatgacg ccttgcaggc cgctgcgaca ttaagcgagc gttatctctc
    10981 cgggcgtcag ttaccggaca aagcgattga tgtgctggat accgcctgcg cccgtgtggc
    11041 catcaacctg tcgtcgccgc cgaagcaaat ctcggcgctg accactctga gccaccagca
    11101 ggaggcggaa attcgccagc ttgagcgcga gcttcgcatc ggactgcgta ccgacacatc
    11161 acggatgacc gaggtgctgg tgcagtatga tgaaacgctg acggcgctgg atgaactgga
    11221 agcggcctgg caccagcagc agacgctggt ccgggagatt attgcgctgc gccagcagtt
    11281 actgggcgtg gcagaggacg atgcggcgcc gttgccggac gcagataccg tggaggatac
    11341 gcagccagag tcagagtcag aacaggataa taccggtgcc gtaccggctg atgaggccga
    11401 cagagaacaa ccggaagaga ccgctgaaac agtttccccg gtacagcggc tggcccagct
    11461 cactgccgaa ctggacgccc tgcataacga ccggttgctg gtctccccgc acgtcgataa
    11521 aaaacagatt gcggcggtga ttgccgaatg gaccggcgtg ccgctcaacc gcctgtcgca
    11581 gaatgaaatg tcggtcatca ccgacctgac gaaatggctg ggtgacacca tcaaaggcca
    11641 ggacctggcg attgccagcc tgcataagca tctactgacc gcacgcgccg acctgcgtcg
    11701 tccgggacgc ccgctcggtg cgttcctgct ggctggcccc agcggtgtgg gtaaaaccga
    11761 aaccgtcctg caactggcag agctgctcta cggcggtcgc cagtacctga ccaccatcaa
    11821 tatgtccgaa ttccaggaga aacacaccgt ctcgcggctg attggttcgc ctccgggcta
    11881 cgttggctac ggtgaaggcg gcgtactgac cgaagcgatt cgccagaaac cctactcggt
    11941 agtactgctc gatgaagtgg aaaaagcgca cccggatgtg ctcaacctgt tctaccaggc
    12001 gttcgataag ggcgaaatgg cagacggtga aggccgcctg attgactgca aaaatatcgt
    12061 cttcttcctg acgtccaacc tcggctacca ggtaatagtc gagcatgccg atgacccgga
    12121 aaccatgcag gaagtactgt atccggtgct ggccgacttc ttcaaacctg ccctgctggc
    12181 gcgtatggaa gtggtgccgt atctgccgct gtcgaaagag acgctcgcca ccattatcgc
    12241 cggaaaactg gcccgtctgg ataacgtgct gcgcagtcgc tttggtgcag aagtggtcat
    12301 tgaaccggaa gtgacggacg aaatcatgag ccgcgtcacc cgcgcggaaa acggcgcgag
    12361 gatgctggaa tcggtcatcg atggcgacat gctaccgccg ctctccctgc tgctgttgca
    12421 gaaaatggcg gctaacacgg cgattgcccg gattcggttg tcggcagtgg acggcgcatt
    12481 tacggcagac gtggaagatg ctcagaacga cgagtccgtc acaaaggatg aaacggtttt
    12541 atgagcaggt tgcgcgtttt ccggcagaca ggatggttta tcgcgggcct gatgacgggc
    12601 ttacccgcca ctgccgcgcc cgcagaagca tcgtcaatgg ccggtgtggc ggtcgccgtc
    12661 gccaccacga ccccgccgga tgcgacagcc acgttacagg ccatgcagtc ctgtcggcgg
    12721 gagtccgcgg cgctggagcg gcttgactgc tatgaccgcc tgctggcacc gctgtccccg
    12781 tcagggtttg acggggcgct ggtcaaagcc ggttttgtgg gcgaagcctg gacccgcgcc
    12841 acggaacagg aaaaacaccg ccagggcaac accacagagc tgctggtgac gcaggtgccg
    12901 ggggaacgcc cgacggtggt gataaccacc ccggctatcg gtcatgttcc gccacgcccg
    12961 gtgctgatgt tcagttgcgt ggacaatatc acccggatgc aggtggcgct gatgcaccct
    13021 ctggatgtcc atgatatcgc cgtcactctg aatgccgaca accgggcatt gcgcagccac
    13081 tggtttgtgc gcgagaacgg caccctgctg gagtccagcc gggggttatc cgggattgat
    13141 gaaattaagc aactgttcgg cgcaaaaacg ctgacggtgg ataccggcgc agacaatgcc
    13201 gccggaaagc tgacctttaa cattgacggg ctggcaaggg cgattgctcc cctgcgcgat
    13261 gcctgccact gggcgggaga ataaaaatat ggcaatggac ttacgcgatc cgaatgtctg
    13321 gatatcgcac ctgctggaaa acctgccgga agagaaactg gcatcggcgc tgaaagatga
    13381 caacccgaac tgggagtata tcgacggcga aatcgtcaag ctgggatcgc tggcccacag
    13441 tcaactggat atcccggaac tccagcgccg ggggctggtc attctggctt cggagagtaa
    13501 ggattttcgt ctgctggcgc atctgctgag aaccctgcaa cacgccggtg atcctcatct
    13561 cgcattgcgc ctgctggcgc tgtatgtgga gcattactgg gcggtggctg cgccgcagaa
    13621 tatggcgcat aaaaaacgct ttgccagcca ggtcattaaa cgctttgaaa caggtatcga
    13681 aggtttttca cagaacgccg ccacagcgca gcgcgatgcg ctgtcagggg aactggcaaa
    13741 actggcgcaa tgctggcagt cacataacgc cccggaactg gcacaggcta ctgatgatct
    13801 ttttgccctg taccagcgtg cgtttaatcg tgcggcaccg gctcctgtcc ccactccggc
    13861 ggcctccggt agttcaccac agaccaccgc cacgtctgaa agcggcgtga cgcaacccag
    13921 tgctccggct ccccaaatcg tcatcgacag tcacgacgac aaagcctggc gcgacacgct
    13981 gttaaaagtg gcggccattt tatgtgaacg ccagccggat tcaccgcagg gctaccgtct
    14041 gcgccgccat gcgctttggc agaacatcac cagtacgccc caggcggaaa gcgatggccg
    14101 tactccactg gctgcggtct ccgccgatat ggtggcagat taccatgctc agctcggtag
    14161 tgccgatatg gcgctgtggc agcaggtgga gaaaagcgta ctgctggctc cttactggct
    14221 ggatggtcac tgcctttctg cacagacggc gctgcgtctg ggttacaaac aggtggcaga
    14281 cgccatccgc gatgaggtca tccgcttcct tgagcgtctg ccccagctta ccgggctgct
    14341 gtttaatgac cacacaccgt ttatcagtga gcagacgaaa caatggctgg cagcttcgcc
    14401 cgacgccaaa gttgcaccgg ttgcgcaaat cggtgaggaa tcgaaggcag ccagagcctg
    14461 ttttgctgag cagggtctgg aggcggcgct gcgatatctg gacatgctac ccgaaggcga
    14521 tccccgcgat cagtttcacc gccagtacct tgccgcacag ttgacggaag aggcggggct
    14581 ggtacagctt gcgcagcagc agtaccggat gttgttcagg atgggattgc agatgatggt
    14641 ggcagactgg gagccgtccc tgcttgagca gctagaacaa aaattcacgg cagaacaata
    14701 aaagagaaca ggagtttttg tgttcagatt accaacaccc cgactactca gcggactcaa
    14761 atcagccctg cgaccggcga tgccccggtt taaagtctct gctttctggc tgctgatact
    14821 ggcgtggatt tttctgctgg tgtggatctg gtggaaaggc ccgacgtgga cgctgtatga
    14881 agaacagtgg ctcaaaccac tggcgaaccg ctggctggca acggcggcgt gggggattat
    14941 tgccctgatg tggcttaccg tccgggtgat gaagcgcatg caacagcttg aaaaaatgca
    15001 aaagcaacag cgcgaggaag ccgttgatcc gctcagcgtg gaactgaacg cccagcagcg
    15061 ttatcttgac cgctggctgc tgcgcctgca acgccatctc gacaaccgcc gtttcctgtg
    15121 gcagttgccg tggtatatgg tcatcggccc ggcgggcagt ggcaaaacga cactgctgcg
    15181 cgaggggttt ccgtccgaca ttatttatgc cccggagggc gcacgcggcg cagaacaacg
    15241 cctgtacctc acgccccatg tcggtaaaca ggcggtgatc tttgatatcg acggcacact
    15301 ctgcgctccc gctgatgcgg atatcctgca tcgccgtctg tgggaacatg ccctcggctg
    15361 gctgaaagaa aagcgcgcgc gccagccgct gaatgggatt attctgacac tcgatttacc
    15421 cgatctgctt accgcagaca aacgccgccg cgagcatctg ttacagacgc tgcgcagccg
    15481 cttgcaggat atacgccagc atcttcactg ccagttaccg gtttacgtgg tacttacccg
    15541 gcttgattta ttgcagggtt ttgccgccct gtttcagtcc ctgaacagac aggatcgcga
    15601 tgcgattctg ggcgtcacgt tcacccgccg tgcccatgaa aatgatgact ggcgaacaga
    15661 gttgaatgct ttctggcaga catgggtgga tcgaatgaat ctggcgttgc cggatctgat
    15721 ggtcgctcag actcacaccc gcgcgtcttt attcagtttt tcccgccaga tgcagggaag
    15781 ccgtgaaccg ctggtgtcac tgcttgaggg tctgcttgat ggcgaaaata tgaacgtgat
    15841 gctgcgtggt gtctatctca cctcttcgct tcagcgtgga cagatggatg atatattcac
    15901 ccagtctgcc gcccgccagt accggctggg caataaccca ctggcgtcct ggcccctggt
    15961 ggacaccgcg ccttatttca cccgcagcct gttcccgcag gcattactcg cagagcctaa
    16021 tctggcaaca gagagccgcg cctggctgat acgttcccgt cgccgcctga cggttttctc
    16081 tgccacaggc ggcgtggcag cactgctgct catcaccggc tggcatcact attacaacgg
    16141 taactatcag tccggcatca ccgtgcttaa gcaggccaaa gcctttatgg acgtgccgcc
    16201 tccgcagggg gaagatgact ttggcaacct gcaactgccg ctcctgaacc cggtacgcga
    16261 tgccacactg gcctatggcg actggggcga ccgcagccgt ctggccgata tgggactgta
    16321 ccagggacga cgtatcgggc cttatgtgga acagacctat ctgcaactgc tggagcaacg
    16381 ttacctgccc tcgctgttta acgggctggt caaagcgatg aacgccgcgc cgccggagag
    16441 tgaagaaaaa ctcgcggtgc tgcgcgtgat gcgaatgctg gaggacaaaa gcggacgtaa
    16501 caatcaggtg gtgaagcagt atatggcaaa acgctggagc gaaaagttcc acggtcagcg
    16561 cgatatccag gcacaactga tgtcccatct tgactacgcg ctggctcata ctgactggca
    16621 cgcagagcgt caggcgggcg acggtgacgc catcagccgc tggacgccat atgacaagcc
    16681 cgtggtatca gcacagaaag aactgagcaa actgcctgtc taccagcggg tttaccagag
    16741 cctgaaaacg cgggcgctgg gcgttcttcc tgccgacctc aatctgcgtg accaggtagg
    16801 gccaaccttt gaccaggtgt ttacatctgc cgatgacaac aaactggttg ttccacagtt
    16861 tcttacccgt tacggcctgc aaagctattt tgtaaaacag cgcgatgaac tggttgaact
    16921 gacggcgatg gattcctggg tacttaacct tacccgcagc gtgaaataca gtgacgccga
    16981 ccgcgcggaa atccagcgcc agttgaccga gcagtatatc agcgactaca ccgccacctg
    17041 gcgggccggg atggacaatc tgaatatccg caattttgag tccatcggac aactgaccgg
    17101 ggcgctggag caggttatca gcggcgacca gcctttgcag cgggcgctga ccgtgctgcg
    17161 tgacaacaca cagccaggcg tcttttctga aaaactctct gccaaagaac gggaggaagc
    17221 cctggcagag ccggattacc agttactcac ccgcctcggg catgaattcg ccccggaaaa
    17281 cagtaccctg gcagtacaga aagacaaaga aagcacgatg caggccgtgt atcagcaact
    17341 caccgagttg caccgctacc tgctggcaat ccagaacgcg cctgtaccag ggaaatcggc
    17401 gctgaaagcc gtgcagttac ggcttgatca gaacagcagc gatccgatat tcgccacccg
    17461 ccagatggca aaaacgctgc ctgctccgct caaccgctgg gttggcagac tgactgacca
    17521 ggcctggcat gtggtgatgg tggaggctgt tcattatatg gaagtggact ggcgcgacag
    17581 cgtggtgaaa ccgtttaacg agcaactggc aaataactat ccgtttaatc cgcgttctgc
    17641 acaggatgcc tcactggatg ccttcgaacg cttctttaaa ccggatggca tactggatac
    17701 cttctaccag cagaacctga agctgtttat cgataatgac ctgagtctgg aggatggcga
    17761 taacaacgtc attattcgcg aagatattat tgcgcaactg gaaactgcgc agaaaatccg
    17821 tgacatcttc ttcagcaaac agaacggtct gggaacatcc tttgccgtgg aaacggtatc
    17881 gctttcaggc aataaacgcc gcagtgtact gaaccttgac ggtcagttag tcgattacag
    17941 ccagggccgt aactataccg cccatctggt ctggcctaac aacatgcgcg aaggcaacga
    18001 aagtaagctg acgctcatcg gcaccagcgg caacgcgccg cgcagtatca gcttcagcgg
    18061 gccgtgggcg cagttccgcc tgttcggggc cggacaactg accggagtac aggatggcaa
    18121 ctttaccgtg cgctttagcg tggacggtgg cgcgatgacc taccgtgtgc ataccgacac
    18181 ggaagataac ccgttcagcg gtgggttgtt cagccagttt ggtctgtcag acacactgta
    18241 ctgaggaggc agggatgaat agtaacgtac tgacacaaac tatcgttacc ggcagtgacc
    18301 cgcgcgggct gccggaattc agcgccatcc gcgaggaaat aaacaaagcc agccacccgt
    18361 cacagcctga gctgaactgg aaactggtgg agtcgctggc gctggcgatt tttaaagcca
    18421 acggtgtgga tttacacacc gccacctact atacgcttgc ccggacacgg acacagggac
    18481 tggcgggatt ctgcgaaggt gcggaactgc tggcggcaat ggtaagccac gactgggata
    18541 agttctggcc gcagggcggc ccggcgcgta ctgaaatgct ggactggttt aactcccgaa
    18601 ccggcaatat tctgcgtcag caaatctcct ttgcggaatc cgacctacca ctgatctacc
    18661 gcacagagcg ggcattgcag cttatctgcg ataagctcca gcaggtggaa ctgaagcgcg
    18721 ttccgcgcgt ggagaatctg ctctatttta tgcagaacac gcgtaaacgg cttgaacccc
    18781 agctgaagag taacactgag aacgccgcac agaccacggt cagaacgctg atttatgccc
    18841 cggaaacaca ggcatcttcc acaccagaag cggtagtgcc tcccctgccc ggcctgcctg
    18901 agatgaaagt ggaagtgcgc agtctgacag agaatccccc acaggccagt gtgataaagc
    18961 aaggcagtac ggtaagaggg tttatcgcag ggatcgcctg ttcagtggct gtcgcctcag
    19021 cattgtggtg gtggcaggtc tatccggtgc agcagcaact gttacaggtt aacgacaccg
    19081 ctcagggcgc agcaacggtg tggatggcct cacctgaact cgaaaactat gagcgcaggc
    19141 tgcaacaact tcttgatacc tccccggtac agccgctgga aaccgggatg cagatgatgc
    19201 gtgttgccga cagtcgctgg ccggaaagcc tgcaacagca acaggcctcg acacaatgga
    19261 atgaggcact caaaacccgc gcacagagta gcccgcagtt gcgtggctgg ttgcagaccc
    19321 gccaggactt acatgctttt gcagatctag tgatgcagcg cgagaaagag ggactaaccc
    19381 tttcctatat caaaaatgtc atctggcagg cggagcgggg actggggcag gaaacacccg
    19441 ttgagtctct gttgacgcag taccatgatg cccgtgcgca gaagcagaat acagatacgc
    19501 tggaaaaaca aattaatgag cgactcgaag gcgtgttaag ccgctggctg ctgctgaaga
    19561 ataacgtcat gccagaggcg gcaaccggta ccacggccga aaaataatta tttaattaaa
    19621 ggagattatc atggcaaata taagttattt atctttgagt ggagagacac aagggctaat
    19681 ttcagcaggt tgctcaacgc ttgattctgt cggaaacaaa gcgcagccag aacataaaga
    19741 tcagatcatg gtttatgcgt taatgcattc catctcaaga agtcaaaatg tcaatcatca
    19801 tgaattaatt atcaccaaac cggttgataa atcctcacca ttgctggcaa aagcacttag
    19861 tgacaatgaa aaaatggcaa tatgtgaatt tata
//
LOCUS       DBANUU010000085        19161 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000085 DBANUU010000000
VERSION     DBANUU010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.085"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            1..965
                     /locus_tag="ACIE68_003040"
                     /pseudo
     CDS             1..965
                     /locus_tag="ACIE68_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
     gene            complement(1227..8302)
                     /gene="yeeJ"
                     /locus_tag="ACIE68_003041"
                     /pseudo
     CDS             complement(1227..8302)
                     /gene="yeeJ"
                     /locus_tag="ACIE68_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
     gene            complement(8616..8691)
                     /locus_tag="ACIE68_003042"
     tRNA            complement(8616..8691)
                     /locus_tag="ACIE68_003042"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8656..8658),aa:Asn,seq:gtt)
     gene            complement(8792..9589)
                     /gene="mtfA"
                     /locus_tag="ACIE68_003043"
     CDS             complement(8792..9589)
                     /gene="mtfA"
                     /locus_tag="ACIE68_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFX5909320.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            9683..9772
                     /locus_tag="ACIE68_003044"
     tRNA            9683..9772
                     /locus_tag="ACIE68_003044"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9717..9719,aa:Ser,seq:cga)
     gene            complement(10342..10872)
                     /gene="yodB"
                     /locus_tag="ACIE68_003045"
     CDS             complement(10342..10872)
                     /gene="yodB"
                     /locus_tag="ACIE68_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFX5909321.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(11215..11865)
                     /gene="zinT"
                     /locus_tag="ACIE68_003046"
     CDS             complement(11215..11865)
                     /gene="zinT"
                     /locus_tag="ACIE68_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFX5909322.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(12122..12757)
                     /gene="msrQ"
                     /locus_tag="ACIE68_003047"
     CDS             complement(12122..12757)
                     /gene="msrQ"
                     /locus_tag="ACIE68_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFX5909323.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(12758..13762)
                     /gene="msrP"
                     /locus_tag="ACIE68_003048"
     CDS             complement(12758..13762)
                     /gene="msrP"
                     /locus_tag="ACIE68_003048"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFX5909324.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(13871..14284)
                     /gene="hiuH"
                     /locus_tag="ACIE68_003049"
     CDS             complement(13871..14284)
                     /gene="hiuH"
                     /locus_tag="ACIE68_003049"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFX5909325.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            14417..15088
                     /gene="hprR"
                     /locus_tag="ACIE68_003050"
     CDS             14417..15088
                     /gene="hprR"
                     /locus_tag="ACIE68_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFX5909326.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            15088..16446
                     /gene="hprS"
                     /locus_tag="ACIE68_003051"
     CDS             15088..16446
                     /gene="hprS"
                     /locus_tag="ACIE68_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFX5909327.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSEGQGLGLSLVKAIAELHGGSATYHYFNKHNVFRITLPQ
                     RN"
     gene            complement(16554..17405)
                     /gene="hchA"
                     /locus_tag="ACIE68_003052"
     CDS             complement(16554..17405)
                     /gene="hchA"
                     /locus_tag="ACIE68_003052"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFX5909328.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            17552..17962
                     /locus_tag="ACIE68_003053"
     CDS             17552..17962
                     /locus_tag="ACIE68_003053"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909329.1"
                     /translation="MLNIIHAKFSLLSNVVASGLNDFRPLSKVRGGGERFSAVKYCGT
                     DEYLYRGLVEKRLFHALFLQIFLYGKRVCYGLIHKEMHLGVGLKSRWLMLLCQEGQGK
                     VLYRYALAIACAADGDEMPIKKPEFDEGRTQASV"
     gene            complement(17996..19159)
                     /gene="ompC"
                     /locus_tag="ACIE68_003054"
     CDS             complement(17996..19159)
                     /gene="ompC"
                     /locus_tag="ACIE68_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFX5909330.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKLDLYGKVDARHTF
                     SDNPGDDGDETIIELGFKGETQITDQLTGYGQALTKTKASDTEGSDNTYVKLAFAGLK
                     FGEMGSFDYGRNYGVIYDVEAWTDMLPVFGGDSYTWTDNFMAGRANGVATYRNSDFFG
                     LVEGLNFALQYQGNNEGSNAGEDQEGTKNGHEDVRFQNGDGFGLSTSYDFDFGLSLGA
                     AYSNSDRTNSQVALGGYHYNEYSKFAGGDTAEAWTFGAKYDANNVYLAMMYAETRNMT
                     PYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYVYSKGKDLGGNDYNNNGHQEYVDQDL
                     VNYVEIGATYYFNKNFSTYVDYKINLLDKDDDFYDNNGIATDDVVGVGLVYQF"
BASE COUNT         4978 a         4292 c         4483 g         5408 t
ORIGIN      
        1 ttgtcatcca tattcccgat cagattggcg atgctatggc catctttcct gttattcggg
       61 cgcttgaatt gcataaaatt aagcatcttt taattgtaac gtcgacaatt aatttagaag
      121 tctttaatgc gcttaaactt gaacagacta aattaacatt agtcacaatg actatgcagg
      181 atcacgcaac attaaaagaa ataaaagatt tagcaaagaa cataacacag caatacggta
      241 cgccggatct ttgcattgag gggatgcgta aaaagaacct gaaaacgatg ttatttatca
      301 gtcagttgaa agcaaaaacg aattttcagg ttgttggtat aaccatgaat tgcttctccc
      361 ctttgtgcaa gaacgcgtcc cgtatggatc agaaactccg ggctcccgta cctatgacat
      421 gggcatttat gatgcgtgag gcgggttttc cagcagtcag gccaatatat gaattgccac
      481 taagtgagga tgtactcgat gaggtgcgcg aggaaatgcg atcgttagga tcttacattg
      541 cgttcaattt agaaggtagc tcgcaggaac gtacattttc attatcgatt gcagaaaatc
      601 taatagcaaa aatttaaagt gaaacagata taccaatagt gatcgttcat ggacccaaag
      661 gtgaagataa agccagggca ttagtggatt gttataataa tgtctaccgt ttatccttac
      721 caccctcgat taaacgttca gcagcaatca taaaagatgc ttatatcgca ataactcctg
      781 acacctcaat attacatatg gcaagtgcct ataatacctc tgttgttgca atttatgctg
      841 attacaaaac gcgatggccc gcaatggccg atgtctcgga gtcagtcgtc gttgggcaaa
      901 aaattgacaa tataagtctg gatgaattcg caaaggcatt aaaaagtgtt ttggcgagaa
      961 tatgagacag tacttatgca tagtaatgcc gatctgttat gaacggtggt gtcatcaata
     1021 ctctgaggct ggtcaatcat cacagaaaac cagcctttag atcaaagcag tactcaccga
     1081 aaatgatcat agtcacatcg aacgcgattc tggcggtgcc gcatttttat ttattcattt
     1141 tctgtgagag aagggctagt tctgtagagg gaatgtaaaa aagagtgccc cgaatggaac
     1201 actctttaag aaattataac ggaaaatcac aggtttttat aacatgtcgc ataagcaaac
     1261 ccaagctttt caaatacgct ttgatcacct gttgccaggg aaactacgta ttgttcgctt
     1321 gagtttgcgg gactagatga ccaatacata tttgattgaa aaccagcgtc agtcgtgtaa
     1381 tgccccatat ctccccattc actgtacagt gacccgatgc ctcgggaact acagtggttt
     1441 aaatagtttg ctctggggag cgtgtagcca ttgctgctgc aaaaattttc agcaaggctg
     1501 tatatcatga aagcctcgcc ggcgttcacc caccaactct tcacacggat ttcgtataca
     1561 tagctagggc ctcctgattt tggcgtcgcc gtaatccttt ccgaattgct gccgacattt
     1621 ttaaatgtca ctttaccggt agcatcaaca tcgacccagg aggcagagct tgaaaactca
     1681 taatcagccg ccgtttttcc tggggcaaag ttgtcattat tcaactgata atacgccccg
     1741 gtgaacccct gcgaagggaa tgtagttgtc ggtaggtcag taccattgac tgatactgta
     1801 ccgctcatta ttttgtcttc tgcgcgagtg aattgtattg tggtactcag acctgcttga
     1861 tgaacaccat tcaatacagg gatcagcgtt gcgataccct ctccgcctcc tgtaacagtg
     1921 gctttgtaat caccgttgat atttagactg taatcaattg cgctaatttt tatatagggc
     1981 acgttagtac ctgattgcac aaattccatc ccttcagaaa ctttgatcgg attgcctgat
     2041 atatcgtgca gaacaagacg taattcagca ctatcggtat agtccccttt cagtgatgac
     2101 cgattgctct taaggacgga ctgcgaggtg tctgccgggc cagccaccag cgttatatct
     2161 actgtttgca ttccaccagc ggtcgtgact gcgcttaccg tgacgctacc tgtcatcgct
     2221 cctttcacgc ttgttgtcgc gattccgttt tgatcggtca ccgctgttaa tgacgttaat
     2281 gtggcagagc cgctttttaa ggcgaagtac acagtgagac cttcgatcag gttaccactg
     2341 ccatcctcaa ccgttgcctt taaggtactt aaatcagtgt tggtggcggc gatagtcgat
     2401 ggatcagcga taaagctagc aacatgggcg gtgcttgagt tgccagtgac tttcacggtt
     2461 gtctgtgtct gtattgttac gccgttatgg aaagatgcag tcaccgtata tgtaccgact
     2521 ttattgctgg tcaaaacgac tggagcctga cctgaagagt tagttctcac ttttccgcca
     2581 cttaacgtcg ccccttctgg cgttacgcta aagttgatga cctgaccctc cactggagtg
     2641 ccatttgcag aggttagcgt tgccgtcaga gtagcgcctg taggggcata gacaccgata
     2701 agcggactgg acgccgtgag tgtcagtttt tcatcaatag cctccagttg tttctcaagt
     2761 gaggctccat tcggcaggga tgctttcacc atatacgaac cgtttctttc gggcgtcatg
     2821 gtgacctcgg ctacaccctg cgtattagta gagaccgtat tctggctgat gatcatttgc
     2881 gaggatggct ctgcactgaa tgtcacgggt tgatgcgcaa ccgggttgcc aaactggtcg
     2941 ttaacgtgag ctttaactgc tacgtcttgt gcgaccatta cctgaccttc cggtatctcc
     3001 agactggtaa tcgtcgcaga attaacatct gcactggcat tcagtaatcg cgagatgact
     3061 tcagtaggtt tatctgccag agaggctgaa actgttttca gtccgacctc ggtgcttgtc
     3121 acaagaattt cagcgaaacc ccgatcatcc gtttcaacgc tggtgctgct tagcgtgacg
     3181 gaggttccgc ggaaattaac ttttatgcct tctaccgggt tgccgttagc gtcagtgacg
     3241 cttgccgtca tggttgcgcc ctcggtcgtg ctgacgacga atgagtatac agaggttaag
     3301 gaggctagtt ttgcggtacc agcatcggca atcaaagtca cctgtttcgt atcactgaca
     3361 ccataattgt tcacgctaac tgtcacgggg taggtgccgg attttgtacc gctcagtgta
     3421 acttcagcct tgccgttgat atcagtaatg gcggaaccgc cttgtccgag agtaaagctt
     3481 gcgctcacat ctgccggtag cgtgaatgtc accgcctcat tggctaacgg gttgccgttt
     3541 ccatcagtca ctgttgcctg cagcctggtc atcccgacgt tattagcgat aaaattgtcc
     3601 tcggtaacgt taagattaac ctgcgcagtg agcgtatccg caataaagtt caccaccaac
     3661 tgctcactct taccgccagt catcgatgct gtaacagtat gagcgcctgc ttttgtacct
     3721 ttcagcgtga ctttcgcttt gccttcagca tcagtaatca ctttaccgcc atcgctcagc
     3781 gtgaagttcg ccttcacatc ttccggcaga gtaaatgtta cctcagtgtt ggctatcgca
     3841 ttgccctctg tatcagcgac tgttgctgtt agtgtcgtga gatcgttatt gtcggcaatc
     3901 accggatcct tcgaggctgc cagcgtgatt tcagcattcg cgacgttcgg cacataggtc
     3961 actgtttgtt gcatcgaatc gccattttcg agggttgccg tcaattgata aaccccggct
     4021 tttgtagcgg taaagcttgc gtaaaaattg ccgtcgtggt tggtagtata tatagcgtta
     4081 ttactggggg tcacgccttc acttggtgaa acgctgaggg ttacctcctg ctgggggaca
     4141 ccgttgccgt aattatcctt cacctcaata tacagactgg ttgtctcgcc tgcggagact
     4201 gtatcaaaaa gtgcctgtag caaggtgaga tgtgccgtag acgcatcagc gttgacatta
     4261 attgatgtgc taagcgtgga gctaccattt tccagactgg cctcaacggt atccggtctc
     4321 gctcctgatt ctatcgagga gcgggtattg gtataagtga cggtagcctt accctgttcg
     4381 ttcgtcacgg cttgaccgcc gttcgtcatt tcggctgtcg ctgcgttgct ggtgaagttc
     4441 acagtcacac ctttcaccgg aaagccatta ttatcaacga ctgtggcggt gatgacgctg
     4501 ccggagctgt tctgcggggt accggcgatt atgctgtctg ggacaggcgt caactcgata
     4561 atttttgccg ccgctgtgtc gccaataaaa tgcacagttt tgttgtcgct ggcaccatta
     4621 ttagccagtg atgcagtaac cgtcttctca ccaaaggcaa cgcctgcgag agtggcctgc
     4681 gcgatgccag actcgttggt attacttact cccggggtca gggtgagtcc tgaagaggct
     4741 gagctgaatg ttaccggaag attattcacc tcattgtcga actgatcttt aaccgttgca
     4801 gttagcgttg cgctatcgac gccattacct gtgatctcat cttttgatat ctgcaggaca
     4861 acctgagccg aggctttgtc cgccacaaat gttaccggct gcgaatcact ggtattgtta
     4921 ttacccagcg ttgcggtaac cgtatgcgtg cccgcttttt tacctttcag cgtgacatgc
     4981 gcttccccat tggcctgagt gatggcaata ccgttatttt caagggtaaa gtttgccgca
     5041 acgtcctgtg gcatggtgaa gtttaccgtt atctccgcca ccggatgatt cgacggatct
     5101 ttcactgttg ctgtcagagt tgtctcatcc acgccattcc caatgatttc cgctttcgat
     5161 gtttgcaaaa cgacaaccgc tctgtcttta tccgccacaa acgtcattgg ctgtgcatcg
     5221 ctgacattgc tgttagccag acgagccatg atcatatgcg tgcccgctaa cgtgccggtc
     5281 agggaggcga ttgcaacccc gttactatcc gtcatgcctt ttcctccgtt gctaatcgag
     5341 aacttacttg cgacgtcgtt tggcacagag aaggtgattt ctttatcttt tagtgggttg
     5401 ccatttttat cctgcaaggt tgcagtcata tattgcggag ctgtgttggt gacggtgata
     5461 tcacctgaag gcacactgag ggtcagggca gcagtacttt gatcaccgat aaaattcacc
     5521 tgttgattag cctgggaacc agagctcaca gaggccgtaa ccctataatc accatttttc
     5581 aaactggtca gcgtagctgt ggcgatcccg tcgtggctat tcacttcggt ttggctcagt
     5641 ttcgcctctg ctgaattaac attgaaagtg accatgacgt cattgagcag gttgcctttt
     5701 gcatcccgga cggtcgcggt cattgtgacg ctgtcattgc cgtcagcaac cacttcattt
     5761 ttcgacttct gcagatcaac ctgcgcggta ctcgagtcgc cgacaaaact gacgattaac
     5821 gtttgtttca cgccattttc aagggtgact tcaaccgtgt tgtcttcctg cttactactt
     5881 ttcagatcaa aagtcgccag accattaaca tccgtttttg cggtgttttg attgttgaag
     5941 gaagttgccg atccgcttaa taccgcaaac gtgacggtat gatcattgat tgggttgctt
     6001 ccttcatcag cgacattgac cgagacggtg tttgctgcat tctcattggc gagcacacca
     6061 ttattgctgg cagataatgt cgcaattttc gctgactgcg ggttggcgtc gatgataaat
     6121 ccagcggtat gcaaatcttc attccagttt tgcattaata gcttcgcagt aagtccactg
     6181 cctttggtat aggcggtata ggtcgcctta tagacgccat ctgcggtttc tttccagtct
     6241 gtagtgactc ctggtttcac gttgtcgatg ctgactgcgt tattcagttg ctgtttttgt
     6301 tccttaacag gtttgtcatt ttcatctctc agttctaccg tcacctcgat aggattgccg
     6361 gagagataac ggtccttatc aatcttaatt gacgagtgag ttcgggatga cgaaacagaa
     6421 atgatattca ccacggcggg ggctttagcc gcatccacac cattcagctg tggcatcagc
     6481 gtcagcgtgc cagacatcgc tcctgtggtc aggatctggg tatagcttcc gtcaccatta
     6541 tctttccagt cagaaagggt gatgtcctga acaccttcgt gacgcgtcga gagcaccagc
     6601 ccgacaacag gattacctgc tgcatcatgc gcaataaaag tcagtgtggc ggttgaatgg
     6661 gaatccgcgt tcaatgtttg ggtacttaac gataccgagg aatctttctg gcttagcgta
     6721 ggtgcctgaa cgaccaccat gctctgttca cgattcgaca aattgccttt gacatcttcg
     6781 gcggtgactt caatcggcca ggtgttatcg gtttctggcg tactggtgaa ccggtaagcc
     6841 ggcagggtaa ccagaatatc tttacccgtt gtgaccactt tgccaccggc agcttccagt
     6901 gcggtggctt cgacgttata gcctttcagg gcatatttgg tttgtagcga cgaaaccagt
     6961 gatttcactt ctcctgactt ccctgtcacg gggtctgtca gggtcaggcg aaccagttct
     7021 tttttgcgat attccagaac gatattgttg ttgcgatcca ccagatcata acggctgcct
     7081 gcaaggctac gccgtgcagc gacttcattc gggtcaagct gtttctgcat tgcgctgcca
     7141 ggttgccagg taaaatcgac ggcaaaacgg gtgtcatttt cgccctgttt accctggcgt
     7201 tgctccgcgc tgaaggtcat cagcgggaag ggggtatagt taagtccagc ggttatggca
     7261 tgaggattac tttgccgatc gtctttatcg aacagggcca cttcatcgcc ataatactgt
     7321 tcatagacca gtttaccgcc aaggtgcggc caggcgggta gccagccttc tgcgcgtaca
     7381 tcccagccat tggccgggcg agcttcataa tcgttgtcca gttcaggtgc gctgcgccag
     7441 ttggtcagtc gcaaatagcc gttactgctt aattttagat agtcgcgcca gtactccgcg
     7501 ccaatgccgg cgcgggagtg gtaacggcta agatcgtggt cgaaaaagaa gttgatgccc
     7561 gacatccatg tgggagtgaa atgacgccaa cctaagccgt tgttaatctg cgtacgctcg
     7621 tcagtacgat gaagagtatg ctgactgaaa aagagattat caggcgtttc atacctcgga
     7681 tggagaaaat cgaactggga gttcttcagg ctaaaatctt catccacgcc cagcgtgatt
     7741 cttgcggtac cgaagcggct taaccagtct gtcattgcgc ctgaagcctg agaagaggcc
     7801 catccacgcg ccatatttgc cgcttgctcg ctgttcatat cctcggcgag cagagacccg
     7861 atttgctgtg aagtactggc tatctgttgc tcaaggttgc catgctatta cccggtggcg
     7921 gggttaaatt attttcacta acttgtgccg ggacatccag ttcatcaccc tggtggacat
     7981 tatcaaaacc tcgagcaaac gtacgaaact ggttgagttt gcgtaactca gccaccgaaa
     8041 taccgaaacg ttcggcaacg ctttgagccg attccaacgc tccaagggtg tagggcaccg
     8101 tattggcatt tgcaatggca atttgtgcag gaactggttg ttgggttgcg gcgtttacca
     8161 caccttgtgc tgccgcagcc atagggaacg caagttgagt tatcagacag atacccgcag
     8221 ttaagcggcg tagtttaatt cccatcccgc ataatatttg tcggtcattt atttcttctc
     8281 cacttctctt cttcgtagcc atgcttacct tccctgaacg agctaacata aatgcgccct
     8341 ttctagcaca gcgaaaaaat gtaaatactg atagcaatat gattgaaaat taataacttt
     8401 tcgttaggca gttttgggtg tgagttgcaa gaggggagac tactgaataa ctcaagtttt
     8461 ataatcgagg ggaaaatggt gatggcgttc atagcaaaac gccctcaacc ataaaggtcg
     8521 agggcgctta agatgttaaa aacccgctat ccgttaaaaa acaatgttca actaaggtca
     8581 gtgacattgc gctaaaaaag cgaattttta gaatttggct cctctgactg gactcgaacc
     8641 agtgacatac ggattaacag tccgccgttc taccgactga actacagagg aatcgtgtga
     8701 acggggcgca tattacttag cggtaccttg tctgtcaaca ctaaatttca taagtcattt
     8761 caattggtta attaatctgc aaagttgtta attaatgaac attcgtcgcc gaaaacgagt
     8821 ctgtatcatt agcgtgatgc agtctctgca aaggatcttg ttgataaaat tggcagaaac
     8881 gttgccacaa tgaagggaaa cgaggagcaa aaagttctgg ggcgctaaag aaatattcag
     8941 aaagtacggc aaaacattca gcaggatcgc tggctgcata agcatcaatg ctcgccgcat
     9001 tctcgccaac caattcgatt tcttcctgaa tgttgttcat tgcagcatga agatcgtgtt
     9061 cccagccagc aacctcacgc aacggaataa agggaactcc gctggcgcga tcgccgttac
     9121 gggtgtccag cttatgagcg acttcatgaa taatcaggtt aaaaccagaa gcatcaaaag
     9181 aatcttgtat atccaaccag ttcaaaacga taggcccttg ctgccagctc tgacctgact
     9241 gaacaatacg ttggttatgc accagaccga tatcgtcttc ccattcatca tcgaccacaa
     9301 atggcgcagg ataaattaag acttcatgaa aaccatccag ccattccagt cctaactcca
     9361 gaacgggtag gcaaaataga agtgctatcc ggcagcttct taatgaatcc agttcaaagc
     9421 cctgtaaagg aacaagccgc ttttgctgta aaaaacgttc ggcaagagtg actaatttgc
     9481 tttgttcctg ttccgtcaga cccgttaaaa gggggatcga tagtgcttcc tgccagggaa
     9541 gggcagtttg atgtgctgat tcttgtactt tccagggcca cttaatcatc gttttgctcg
     9601 caaactcgtc acttgaacaa aattgcacgg acagggactg ttaaaatgcc aaatttcctg
     9661 gcatcatggc aaccatctga acggagagat gccggagcgg ctgaacggac cggtctcgaa
     9721 aaccggagta ggggcaactc taccgggggt tcaaatcccc ctctctccgc cactttatca
     9781 atgacttatc tcctgacttc ccgccttgct tttcctaaac agaacaatcg tagaatattc
     9841 ttgaagggtt agatcgtcac tgttttctgt tcgatactgt gacattcagc acttgattcg
     9901 ctatggatct gacaggaagc tttcgagcga aaatgtgcag ttattcagtc gttttcttat
     9961 cgctcaccat tattctttta gacattcatc ctatgaaact gccgcaaagt tggtggtggg
    10021 aactgaagtt gcgtagagaa gggttcaata attgagtcaa aaaaggaacg cggccgtcag
    10081 attaaagccg cgggacaaag tccataattt ccaagagttg ttgaatttcc cagctttttt
    10141 gaaagccttt gtatcatact aacaagtaaa aaaggtttaa ataccaaaat taacctgtat
    10201 ttttgataag tcttaccatt gtacgattta cacagtttct gctcctcctt gttgatagtg
    10261 gaaagggtgc attgtgcact tgcgtaaaca tttatgacgc gttgatgtcc atggttcaca
    10321 tttccttgtc aggggatcga cttatttgcg gcgaggcatc attcgtaaca gagtattatc
    10381 tttctgaatg tagtgatgaa agagtgctgc gccagcgtga gctgcgatta aaaagtaacc
    10441 tatatttgcc caggtttcgt gaatattttt tatcagtgct ttaatttcgc tgtttggggt
    10501 aacaaaggga gacacattga aaccaaggaa actccacgat tttccactgt aagccatcaa
    10561 agcaatcccc agcagaggaa gtgcaaggaa ggttatataa agcatgatgt gcattagcga
    10621 agccgctttc atttgccagg caggtggcgg tggcacaata gaaggatcac tataacggtg
    10681 ttttataatc aggcgtgaaa acattaacac ccaaacgaat atacccgcat tgtaatgtgt
    10741 ttctcgcatc agcagataag tactactacc tttaggaaac cagccacgga gttccatcgc
    10801 ggcataggtt attgcaacga aaagcagcgt tatccaatgt aaataaattt gagtttttga
    10861 gaatcggttc atttttgtag ctcctgtaat ttttacagtc aagctatccc acgaacctta
    10921 aaaattcctt atacagatgt ggttttaaga atttgttatt taaaaaattg atagtgcaca
    10981 ttgtaaccag ccaatataag caatggcgtg agcacaggta tctttctctg ttggccgtat
    11041 tgtgtatgga atccgttatt ggttaactca atgcgggtta gcttattaat atacagacac
    11101 gtcatagtga tgagcgtgtc tgtagctagc ttgcgttcag tggcgtttgt ggatggacca
    11161 gtggcagtta tgatgactta ctaaagcggc atcgaggcgt tatcatgaga atactcaatg
    11221 agacatcatt tcctcgacca cttcttcgct actcaactga tatggataat acgttggcca
    11281 gttttccatt tcatttaata aagattgctg tgaatcatta cccataaaaa tgtgaaaatg
    11341 agatgatttt cgtggtgcaa ttatatgatc gctaaattgt atatatttag gggctttgct
    11401 ttcaggatct ttacattcga ataagtaacg aacgcctttc ttgcctgatt tataggtgag
    11461 tattttgtat ccatcgtaat catatttaca ggatgttgtt tcattatttc tatggaattc
    11521 aacaatgccg tcctcaatgc caatcatctc gatatctgtt gcataacctt tgtgataata
    11581 atctttaatt tcagcaaatg ttttagtttt atctgcatcc gctttcttct gaaagacggg
    11641 gtcaagtttg ccactttgca gtaaaggata aacggattgc cagactccat cccagtcact
    11701 gagcgttcgg ttttgtacat tggcatcatc aaaaacacca ttagcagctt tttgttcgac
    11761 ctctgttaag ggtttgccgt gtgagtgatg accatgcgaa aaggcaggag cgctaacaat
    11821 aaagacacct aaagcaacag ccagtttgtg aagacgaatc gccaaaacag tttcctccag
    11881 agtcattgta tatgatgtgt aatgttatat tgtaacatat ggcaaaatgc aacaactctt
    11941 aggagatgag agcatagttt tctcaggtgt acttatctat cttattgaaa taaaatgaat
    12001 tatattgata atttgaggtt gatgacaaac gaaacataat ctgatgtgat ctgcgtaggc
    12061 cggatagggc gctcgcgccg catccggcag ggataacgcg tactttgtcg gcaatatgaa
    12121 gttatacaga taatttgttg tgaacctgct tgcgtaaccg gttaaaaagg gaacgcaact
    12181 tcttataccg taaggctaaa agcagtacag ccagcccagc gtagatgagc ggctgcggtg
    12241 aaataatctt cactgaccac agataatgta ttggggccag gatcgcgaca agatagacga
    12301 aattatgcaa ctgttgccag tgcttgccga gttttcgctg catcgcctgg gttgaagtaa
    12361 acgctaaagc aagcaaaata acccagctga taattcctaa cgttaaataa ggccgggtaa
    12421 ttaactcctt gcctaataac gccagattat tcacgcccag ctccagcaat gcgtaactgg
    12481 ttaaatgcag tgtcgcccag gcaaagcacc ataagcctaa caggcggcga gtgcgtatca
    12541 ataacggctg ttttgcgtag cgtgccagag gtgtgattaa caaggtcgcc agcaaaaatt
    12601 tcagtgcagt gcgaccggta aaatgctgaa tatctttcac cggatcggca cccagtccac
    12661 cgtgattgat cgcccagacc agccagagaa acggcaacaa tccggcaaga tgcaggctaa
    12721 ctttcagcca tgtcacctgt tttgctgtca gacgcattca gaaattctcc cgcaaatcca
    12781 ggccacgata cagcgatgcc acctggtcgg cgtaaccatt aaacagtagc gttggctggc
    12841 gctgaacatc gaggatgccg cctgaaccaa taaatcgttc ggtagcctgt gaccagcgcg
    12901 ggtgatcaac atgcggatta acgttggcgt aaaaaccgta ttcgtcaggc gctgccagat
    12961 tccaggtggt tggcggacgc tcgcgggtca gcttaatact gactatcgat ttaatccctt
    13021 taaagccata tttccacggc acaatcagtc gtaccggcgc gccattttgt ggcggtaacg
    13081 ccttgccata aacacccacg gtcatcagtg tgagcggatg cattgcttcg tcgagacgca
    13141 atccttcgac ataaggatat ttcagcccgc cgccgataaa gcggtcctgc tggcctggca
    13201 tttgttccgg tgcataaatt gtttcgaaag cgacatactt cgcattgctg gtgggttcgg
    13261 caagcgccag caatttgtgc agcggaaaac caatccacgg caccaccatc gaccatgcct
    13321 ccacgcagcg catacgataa atacgctctt ccagcgggaa gcgacgggtt aaatcatcgt
    13381 gatcgagggt caatggcttt gccacttcgc cgctgatttt cagtgtccat ggatcggttt
    13441 tcaggctacc agcattagcg gcgggatcgg ctttatccag cccgaattca tagaagttgt
    13501 tataaccgga gactttatct actggcgtca gtggcagatt attttgccag gcggcaggct
    13561 tgctgaactc cagcggtttt ccggcggggg cgggcgggcg atcgttccct ttaaaccagc
    13621 taagcagatc ggcatgcgca gcgtgaggca aagaaagtgc agctgcgctg atgcccagtg
    13681 cttttaacac ctgtcgacgc ttcataaaga ataccgactc ggccgtaaca tctgattctt
    13741 ttaaaaattg attctttttc ataagccatc acactttttt gtcgttagcc agcaagagcc
    13801 gtttactgtt aatctgtcag ggagtgtcct ccctaacagt agcagagatc tgcttttaca
    13861 tatttatgca ttaactgcca cgataggttg aatacccata ttggctaagt aataaaggca
    13921 catgataatg ctcgttcact ttattaatat gaaattcaac ggggatctca gggaagaaac
    13981 tttcaagatt ttgtttcttg aaatagtccc cggttttaaa tacgacacgg taatcgcccg
    14041 tagttgcagt ttgctcgggc cacagtgcct taattcgtcc atccttatct gttttggcgg
    14101 tattaagttg taaccagccg ttgtccgctt tcttttcaag agtgactgtc acgtcggcag
    14161 cgggttttcc ggtttgctgg ttcaaaatgt gcacgctaag aatgttttgt tgtgcggcat
    14221 aaaccaaaga aggtaatgaa aatgctgccg ttactacgga gagtactaaa taacgcttta
    14281 acatctttat atccttgtca tgtgaatgag ttttgaggat agcgaaaaag cggccagggt
    14341 aaccttacag cagcattaca attttgtaat gaaccaggct gtttctataa catatgattt
    14401 atggcatatt attttcatga agattttact tattgaagat aatcaaagga cccaggagtg
    14461 ggtaacgcag gggctttccg aagcgggtta tgtcattgat gccgtttctg atggcagaga
    14521 tgggctttat cttgcgctga aggatgatta tgcattgatc attctggata ttatgcttcc
    14581 gggtatggat ggctggcaga tcttacaaac gttaagaaca gcaaagcaaa cccctgttat
    14641 ttgccttact gcaagggatt ctgtcgatga cagagtcaga gggctggaca gtggagcaaa
    14701 tgattatctg gtaaaacctt tttcattttc tgagttgctg gcaagggttc gggcacaatt
    14761 aaggcaacat cacgctttga attcaacatt agaaatcagt ggcttaagaa tggactctgt
    14821 aagtcaaagt gtgagcaggg acaatatcag tattacactg acgcgcaagg agtttcaatt
    14881 actttggcta ctggcctcca gagctggcga aattataccc agaacggtta ttgcgagtga
    14941 aatttgggga atcaactttg atagtgatac caatacggtg gatgtcgcca ttcgcaggct
    15001 ccgcgcaaaa gttgatgatc cttttcctga aaagctaatc gccacaatcc gggggatggg
    15061 ctattcattc gtagcggtaa aaaaataatg aaaagattat ctataaccgt ccgtttaacc
    15121 ttgcttttta tattgctgct gtctgttgct ggcgctggaa ttgtctggac tctctataat
    15181 ggcctggcaa gtgagttgaa atggcgcgat gatacaacac tcattaaccg gacagcacag
    15241 atcaagcagt tgttaattga tggggtaaat ccagatacgt tacctgtata ctttaaccgg
    15301 atgatggatg ttagtcagga tatcttgatc attcatggtg atggcatcaa taaaattgtt
    15361 aaccggacaa atgtcagtga tgacatgtta aataacatac ctgctagtga gacaatcagc
    15421 gcagctggca tttacagaag cattattaat gatactgaga ttgatgcttt acgaattaat
    15481 attgatgaag tttcgccatc attaacggtt actgtggcta aattggcttc agccagacat
    15541 aacatgcttg aacagtataa aattaatagc attataattt gcattgtcgc cattgtactt
    15601 tgctcagtat taagtccgct gttaatcaga acgggattac gagagatcaa aaagttgagt
    15661 ggtgtaacgg aagcgctgaa ttataacgat agccgggagc ctgttgaggt tagcgcatta
    15721 ccgagagaac taaaacctct tgggcaggcg ttgaataaaa tgcatcatgc tttagtcaaa
    15781 gattttgagc gtctaagtca atttgctgac gatctcgctc atgaacttag aacgcccatt
    15841 aatgcattac tgggtcagaa tcaggttacg ctcagtcaaa ccagaagtat cgctgaatat
    15901 caaaaaacaa ttgccggtaa cattgaagag ctggaaaata tttcgcggtt aacagagaac
    15961 atactgtttc ttgcccgggc agataaaaac aatgttttgg tgaaactgga ctcgctttct
    16021 ctcaataagg aagtcgaaaa tttgttggat tatcttgaat acctttcaga cgagaaagag
    16081 atttgcttta aggtcgagtg caatcagcaa atctttgcgg ataaaatttt actgcaacga
    16141 atgttatcga atcttattgt taatgccatt agatattcac cagaaaaatc gcgtattcat
    16201 ataaccagtt ttcttgatac caacggctat cttaatattg atgtcgccag tcctggaacg
    16261 aaaattcatg agcctgaaaa actcttccgt agattttggc ggggagataa ttcgcgtcat
    16321 tccgaaggtc agggacttgg tctttcttta gtcaaagcga ttgccgaatt gcatggggga
    16381 agtgctacgt atcactattt taataagcat aatgtgttcc gaattacgtt accgcaaaga
    16441 aattaacata gggcttcagt acgccataaa tcaaccatga aaaaacgcag ccacgtcgtg
    16501 agtattaacg tggctgcgat tgattatgcg cttacattca tacgtaacag ggattaaccc
    16561 gcataagctg ccagcatttc ctgcgccgcc agtttaccca acgcattcgc tgcgaaagga
    16621 ctgtcgccgg tgagaacttt acggtcctta tgtactcgcc cggtgatgtc gtcattaata
    16681 atgttcatgc ccattttctt cagctcttcg ccgaagtacc aggtgagatg acccggcata
    16741 tagcctatct ctggcgtttg tttgtctgcg gcgtctggga atgcgcaaat ggaataacca
    16801 ttcagtgggt tatcgccgtg gcgaagcgcc agaaaagccg ccgggccgtg gcaaagggag
    16861 ataacgaaac ggtcattttt gattgcccac tgtaaagcgg cagctacgtc ctggctttca
    16921 ggtaaaccaa taagtgcgcc atgaccacca ggaacaaaga ttgctgcata ttcgctatca
    16981 gcgttgaggc tggcgacaac atccgcgagt ttcttcggat tgcggaacaa cgatttatgc
    17041 tgctcaaaga atggcattac tttttcatct ttgtgcggca tagcccagta ttcaaacttg
    17101 gtcatcagac cggaaatggt cgccacttcg aattcgaaac ctgcagcatg gagatgatac
    17161 aacggcagca acgtttcaat cggatggtta ccggtcgaga acagttttcc gttatcggtt
    17221 ggcaaataac gttcgtccgc ggcgatcacc agaattttat gcttaccgcg atacggtttt
    17281 ggatagtcca cgccatcaag atcagagacg ggactggtat attggctaag cgaatattct
    17341 gaagggaaga atgcattatc ttcagcaata tcgacctgcg gatttttact tgtttgaaca
    17401 gtcatagtgt attccttatt gttgcttagt tagggtagtc actatatttg cgactgagct
    17461 ggtcgctatt tttaaccagt ctggaaaatt gagtcaatat atcgaaagcg gtagtcggcg
    17521 ggtgtaagta tgtaatgatg atgctgcact ggtgttgaat attattcacg ccaaattttc
    17581 cttattgagc aacgtcgtcg caagtggcct caatgatttt agaccattat caaaggtacg
    17641 gggcggcggg gagagattta gtgctgtgaa atattgtggt actgatgaat atttatatcg
    17701 cggattggta gagaaaagat tatttcatgc actatttttg caaatatttc tatatgggaa
    17761 gcgtgtttgt tacggattaa ttcataaaga aatgcatttg ggtgttggtt tgaagagtcg
    17821 ctggttgatg ttattgtgcc aggaggggca gggtaaggtg ctgtatcgat atgcattggc
    17881 aattgcgtgt gccgcagatg gagatgaaat gccaataaaa aagcctgaat tcgatgaggg
    17941 acgaactcag gctagtgttt agttttacag taacacgccg cagcacgttg gctgattaga
    18001 actggtagac taagcccaca ccaacaacgt catctgttgc gatgccattg ttgtcataga
    18061 aatcgtcatc tttatccaac aagtttattt tatagtcaac atatgttgag aagttcttat
    18121 tgaaatagta ggtcgctcca atttcaacat agttgaccag atcctgatca acatactctt
    18181 gatgaccatt gttgttatag tcgttaccgc cgaggtcttt gccttttgaa taaacatatg
    18241 caagagatgg gcgcagacca aaatcgaact gatactgtgc tacagcttca aagttctggg
    18301 ttttatttgc gatacccaca ttaccatatg gtgtcatatt gcgggtttca gcatacatca
    18361 ttgcaagata aacattgttg gcgtcgtatt ttgcaccaaa tgtccatgct tcagctgtat
    18421 caccaccagc aaatttgctg tattcattgt agtgataccc gccaagagct acttgagaat
    18481 tggtacggtc agagtttgag taagcggcac ctaagctcaa accgaagtca aagtcatatg
    18541 aagtggaaag accaaaacca tcaccgttct ggaagcgaac gtcttcgtga ccatttttag
    18601 taccttcttg atcttctcct gcattgctgc cttcgttgtt accttgatac tgcaatgcaa
    18661 agttcagacc ttccaccaga ccgaagaaat cactattgcg gtaagtagct accccatttg
    18721 cacggcctgc catgaagtta tcagtccagg tataggagtc gccgccgaat acaggaagca
    18781 tatctgtcca ggcttcaacg tcgtaaatca caccatagtt acgaccatag tcaaaggagc
    18841 ccatctcgcc gaacttcaga cctgcaaaag caagtttgac ataagtatta tcagaacctt
    18901 cagtgtcgct agcctttgtt ttggtcagtg cttgaccata accagttaac tgatctgtaa
    18961 tttgggtctc acctttgaag cctaactcga tgatagtttc atcgccgtca tcgccagggt
    19021 tatcggaaaa agtatgacga gcatcaactt ttccgtacag atccagttta ttgccatttt
    19081 tattataaat ttcagccgca tttgctgcgc cagcaactaa taacgccggg accagcattg
    19141 ccagaacttt tcttttcatt a
//
LOCUS       DBANUU010000086        18668 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000086 DBANUU010000000
VERSION     DBANUU010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.086"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            249..941
                     /gene="yieP"
                     /locus_tag="ACIE68_003055"
     CDS             249..941
                     /gene="yieP"
                     /locus_tag="ACIE68_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFX5909331.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
     gene            964..2391
                     /gene="mdtD"
                     /locus_tag="ACIE68_003056"
     CDS             964..2391
                     /gene="mdtD"
                     /locus_tag="ACIE68_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFX5909332.1"
                     /translation="MSDKKNRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(2357..3349)
                     /gene="rbsR"
                     /locus_tag="ACIE68_003057"
     CDS             complement(2357..3349)
                     /gene="rbsR"
                     /locus_tag="ACIE68_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFX5909333.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLSIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(3353..4282)
                     /gene="rbsK"
                     /locus_tag="ACIE68_003058"
     CDS             complement(3353..4282)
                     /gene="rbsK"
                     /locus_tag="ACIE68_003058"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFX5909334.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            complement(4408..5298)
                     /gene="rbsB"
                     /locus_tag="ACIE68_003059"
     CDS             complement(4408..5298)
                     /gene="rbsB"
                     /locus_tag="ACIE68_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFX5909335.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            complement(5323..6288)
                     /gene="rbsC"
                     /locus_tag="ACIE68_003060"
     CDS             complement(5323..6288)
                     /gene="rbsC"
                     /locus_tag="ACIE68_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFX5909336.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            complement(6293..7798)
                     /gene="rbsA"
                     /locus_tag="ACIE68_003061"
     CDS             complement(6293..7798)
                     /gene="rbsA"
                     /locus_tag="ACIE68_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFX5909337.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLGKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            complement(7806..8225)
                     /gene="rbsD"
                     /locus_tag="ACIE68_003062"
     CDS             complement(7806..8225)
                     /gene="rbsD"
                     /locus_tag="ACIE68_003062"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFX5909338.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPISKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            complement(8392..10260)
                     /gene="kup"
                     /locus_tag="ACIE68_003063"
     CDS             complement(8392..10260)
                     /gene="kup"
                     /locus_tag="ACIE68_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFX5909339.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10483..11979
                     /gene="ravA"
                     /locus_tag="ACIE68_003064"
     CDS             10483..11979
                     /gene="ravA"
                     /locus_tag="ACIE68_003064"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFX5909340.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            11973..13424
                     /gene="viaA"
                     /locus_tag="ACIE68_003065"
     CDS             11973..13424
                     /gene="viaA"
                     /locus_tag="ACIE68_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFX5909341.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(13429..14421)
                     /gene="asnA"
                     /locus_tag="ACIE68_003066"
     CDS             complement(13429..14421)
                     /gene="asnA"
                     /locus_tag="ACIE68_003066"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFX5909342.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKVLPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            14573..15031
                     /gene="asnC"
                     /locus_tag="ACIE68_003067"
     CDS             14573..15031
                     /gene="asnC"
                     /locus_tag="ACIE68_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFX5909343.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15121..15564
                     /gene="mioC"
                     /locus_tag="ACIE68_003068"
     CDS             15121..15564
                     /gene="mioC"
                     /locus_tag="ACIE68_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFX5909344.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKDSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            15943..17832
                     /gene="mnmG"
                     /locus_tag="ACIE68_003069"
     CDS             15943..17832
                     /gene="mnmG"
                     /locus_tag="ACIE68_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFX5909345.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            17896..18519
                     /gene="rsmG"
                     /locus_tag="ACIE68_003070"
     CDS             17896..18519
                     /gene="rsmG"
                     /locus_tag="ACIE68_003070"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFX5909346.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         4686 a         4866 c         4614 g         4502 t
ORIGIN      
        1 cggaattctc aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt
       61 ggtggcgcat tatagggagt tattccggcc tgacaagagg aaatttaaaa taattttctg
      121 accgcgcatt ttttcagcaa aagccccgtt aatgtgccat aaaacaagca atttgccgtt
      181 tttgcaaccg taatcacact tcctggtggc atactaagga ggaataaaaa gaaaaaggaa
      241 gattagtaat gcctttaagc gcacaacagt tagctgcaca gaaaaacctt tcgtatgttc
      301 tggctgagaa gctggcgcaa cgcatcttaa aaggtgaata tgaacccggc accattttgc
      361 ctggtgagat tgagctgggc gagcaatttg gagtgagtcg tacagcggta cgcgaagcgg
      421 tcaaaacgtt aacggcaaaa gggatggttt taccgcgacc gcgaattggt acccgggtca
      481 tgccacaatc aaactggaat tttcttgatc aggaattgct tacctggtgg atgacagaag
      541 agaactttca tcaggtcatc gatcactttc tggttatgcg tatctgtctg gagccgcaag
      601 cttgcctact ggctgcaacg gttggcaccg cggaacagaa ggcgcatctc aatacgttaa
      661 tggccgaaat ggcggcatta aaagagaact ttcgccgtga acgctggatc gaagtcgata
      721 tggcctggca tgagcatatc tatgaaatga gcgccaatcc gtttttgacc tcattcgcct
      781 cgctattcca ttcggtttat cacacttact tcacgtcaat taccagcgac acagtgataa
      841 agctggatct gcaccaggcg attgtcgatg cgattgtcca aagcgatggc gacgcggcat
      901 ttaaagcttg tcaggcgctg ctacgctcac ctgataagtg acaacccgat aacaggacat
      961 tgaatgagcg ataaaaagaa tcgcagtatg gcgggtttgc cgtggatcgc ggcgatggcc
     1021 ttcttcatgc aggcacttga tgccactatt ctgaataccg ccttacccgc aatcgctcat
     1081 agccttaatc gttctcctct cgcgatgcaa tcagccatca tcagttatac gctgacggtg
     1141 gcgatgctta ttccggtaag cggatggcta gccgatcgct tcggtacgcg tcgcattttt
     1201 acccttgccg tgagtctgtt cacattgggt tctctggcct gcgcactttc taattcgcta
     1261 ccacagctgg ttgtcttccg ggttattcag gggataggcg gcgcaatgat gatgcctgtt
     1321 gctcggctgg ccttactgcg cgcttatcct cgtaatgaac ttcttccagt attgaatttt
     1381 gtcgccatgc cgggtctggt ggggccaatt ttaggccccg ttcttggcgg cgtgctggtc
     1441 acctgggcaa cctggcactg gatattttta atcaatatcc ccataggtat tgcgggcctt
     1501 ctttacgcgc gcaaacatat gcccaatttc accaccgcac gacgcagatt cgatatcact
     1561 ggctttttgc tgtttggcct cagccttgtt ctcttctcaa gcggaataga gctattcggg
     1621 gaaaagattg tcgccagctg gattgccttg acggtaattg tcaccagcat cgggttactg
     1681 cttctctata ttctccatgc gcgacacacg ccaaacccat taatttcatt agatttattt
     1741 aaaacccgca ctttctcgat cggtatcgta ggcaatattg caacccgtct ggggaccggt
     1801 tgtgtaccgt tccttatgcc attgatgtta caggtaggat ttggttatca ggcgtttatt
     1861 gccggctgta tgatggcacc gacagcgtta ggttccatta ttgcaaaatc gatggttacc
     1921 caagtcttac gtcgtctggg ctatcgccat acgttagtgg ggatcacggt gattattggg
     1981 ctaatgatcg ctcagttctc tttgcaatca ccggcaatgg cgatatggat gctgatcttg
     2041 ccgttgttta tattagggat ggctatgtcg acgcaattta ccgcgatgaa taccatcaca
     2101 cttgccgatc tgaccgatga caacgccagc agcggtaaca gtgttctggc ggtcacgcag
     2161 caactgtcga ttagtttagg cgttgctgta agtgcggccg tccttcgcgt ttatgaaggg
     2221 atggaaggca caacgactgt cgaacaattc cactatacgt ttatcacgat gggcattatt
     2281 actgttgctt cagcagcaat gttcatgctt ctgaaaacaa ccgatggtaa taatttgatc
     2341 aaaagacagc gtaaatctaa gccgaaccgc gttccatcag aatcggagta agttgtaatc
     2401 gttgttgctg aagggtcggc tgggttatcc gatggatgag tacatcaatc gccagctccc
     2461 ccagttcatc tttcggttgg tggatagtgg ttaatggtgg cgtcataaag cttgccagtt
     2521 cgatatcgtc atagccaatc accgcgatat cctgcggaac ctgtaactct gcctgatata
     2581 acgcctggta aacgccaaca gccatagcgt catttccggt aaagacggcc tgaggacgca
     2641 gcggatgtga taacagttgg cgcatggcgt caaacccgcc gttaaattca aaatcaccag
     2701 tgacttcata gccatcagga atgctgagac ccgcacgttt catcgccgcc cgataacctt
     2761 ccaaccgcag gcgcgccgga gttttatcca gcggaccggt aatacaggcg atacgggtat
     2821 gacctttatc gatcagatat tgcgttgcta agtctccgcc cagcaacgag ttatcctgaa
     2881 taagatcgct gtcgccatcg aacggagccc agtccatcat cacagtaggc actgtcggat
     2941 aacgttgcat gatttcacgc gaaggttgat gcgtttcggt gcacagtaac agcaagccat
     3001 caacgcgttt ttgcatcagc gtttccagat tgcgattcat ccgctgttca tcgccttcgg
     3061 tattgcaaag gacgagacta taaccgcgtt cgaagcagct gcgttcaacg ccacgcacca
     3121 gttctgaata gaaaggattg gtactggcag tgatcaacat gccaatggta tgtgtttgat
     3181 tgagtttgag gctacgcgcc agggctgatg gcgcgtaatt gagttcttta atcgccgctt
     3241 caactttggc ggtaatcgct tcactgacga agcgatcttt attgataacg tgagaaactg
     3301 ttgaggtaga aacgcccgcc aggcgggcaa catctttcat tgtagccaag cgtcacctct
     3361 gcctgtctaa aaatgcgtcg atctcttcac gccacggtac ggaaggttgt gcgcctttac
     3421 gtgttacggc aatcgcagcg gcagcatggg caaaccgaat cgcctctggc aatggttttt
     3481 cttccagcaa cgccgtgatt aacgcgccgt taaaggtatc tccggcagca atggtatcga
     3541 cagcctgcac ccggaatcca ggaacgcgct gaccttcacc attcacgcta gcccatacac
     3601 cacgacttcc taaagtaatc agtacagtac ggataccttt ttcatgcagt acctgcgccg
     3661 ccttcgctgc atcttcatca ttttcaacac gaataccggt gagcttttct gcttccgttt
     3721 cgtttggcgt aataatgtcc accagcgcca gcagttcgtc aggaagttcg cgagccggag
     3781 ccgggttaag cgcaacgata gtcttatttt gatgggcgat tttcgccgct gccatcacac
     3841 tttcgagtgg tgattccagc tgcattaata atgctgacgc gttggcaata cgctcacgtt
     3901 gcgcttccac cagcgccggg gaaagggcag cattagcgcc ggcatgaata ccgatgacat
     3961 tctcaccttc gccattaaca aaaatcagcg ccacacctgt tgattcgcct ttgatcacgc
     4021 tgaccggagt aatatcaatg ttatcagtgg cgagctgctg gcgaacgctc tcaccaatgc
     4081 tgtcatcacc cgtacaggca ataaacgcga tattcgcacc gctacgccca gcagccacag
     4141 cctgattcgc gcctttgccg ccaaatgcaa cctgatagtg gttaccggtt acggtttcgc
     4201 ctggagtagg aaaagattga agattaagaa tgtggtcagc attaatgctg ccaagaacaa
     4261 cgaggctgcc tgcgttttgc atattcggga tgtccataaa atgcgccacc gtgttagggt
     4321 ggcgcgtgcc ctgcttttct ttatattaca cgttcatctg tcgatgacgt atttatgtca
     4381 ccatcaggtc atacaacctg attaaaacta ctgcttaaca accagtttca gatcaaccgg
     4441 atacttagcc tgaactttct cgcctttcag cactttatct gcggtttcga cgcctttcgc
     4501 gccaatctga tcgggtagct gagcgatagt cgctgctagt ttgccatcat tcaccgcttt
     4561 ttcgccatcc ggtgtaccgt caaatccgac gaccatcaca tccgatttac cggcagtttg
     4621 cagtgcgcgc agcgcgccca gcgccatttc atcattctgc gcgaatacag cctgaacatc
     4681 cggatgagcg gtcaacaggt tctgcattac gttcaaacct ttagtgcgat caaaatctgc
     4741 tggctggctg gcaagaacat taaacttgtg agcagcaacg gcctgctgga agccttcgcc
     4801 acgttcacgg gctgcggatg taccagcaat gccttgcagc tcgataactt tggcaccttc
     4861 acccgctttc ttcgcgatgt aatcaccggc gattttgcca cccagtacgt tatcagaagc
     4921 aatgtggctc accacttcac ctttcgttgc ctggcggtca agagtgataa ccgggatgtt
     4981 cgcctggtta gccatcttca cagcattacc tactgcgtcg gagtcggtcg ggttaatcag
     5041 cagaattttg gtgccgcgaa cggttaagtc ctgcacgttc gccagctctt tcgccgggtt
     5101 gttctgggag tccagcacca ccaggttata gccaagctta tccgcctctt tctgcgcgcc
     5161 atctttcagc gatacaaaga acgggttgtt aagcgtggag accaccagtg cgatggtgtc
     5221 ttttgccatc gcattcgcac tgacggtggc gcttagcgca acagcggaaa ccagggtagc
     5281 cagttttttc atgttcatat tcaagatgtc ctgtagtcgt tattactgct ttttgttgtc
     5341 taccagcacc gccagcaaaa tcaccaccgc tttgacgatc atctggtaat aggaggaaac
     5401 acctaacaaa ttcaatccat tattaaggaa gccaagaatt aatgcgccga tcaacgtccc
     5461 aacaatgcga ccttttccgc ccgccagact cgtaccgccc agaaccaccg cagcaatagc
     5521 atccagctca tagccagtcc ccgccgtggg ttgtgcggag gagagacgcg ccacttcaat
     5581 gatcccggcc agcgatgcca gcagaccaca aagagaatag acgatgattt tgattttatt
     5641 gacgttgata ccagaaagac gcgttgctgc ttcgttgccg cccagcgcgt agatgtaacg
     5701 ccccagacgc gtgtgatgca gcatgtacca ggccgcgagg aagacaatcc ccatgatcca
     5761 gactggcgtc ggtacgccca gcggacgacc aataccaaac cagccaaaca gatcggcgtt
     5821 ctcagtaaag ccggtattca ctgggctacc gttggtataa accatggtca cgccgcgcag
     5881 taaaagcatc ataaccagcg tagcgataaa cgcctgaacg cgacctttcg ctacaatcac
     5941 cccggttacc gcaccaattg cggcacctaa cgcgagagcg gcagcgacag ccaccagcgc
     6001 attgacttca atgccgacga tagatgcagc aactgcgccg gtcagcgcca acagagaacc
     6061 taccgacaag tcgatacccg acgtcaggat caccagcgtc atcccgaccg ccataatggc
     6121 gttcaccgag gtttgctgaa gaatattgaa taagttattg atggtgaaaa agttcgggct
     6181 taacgtcgag acaatcgcga tcagcaccag cagagcgata agcgatttct gctccatcag
     6241 ccacgctttc gtgaaataac ggcgaccaga gacagtctgg gttgtcattt ttttactcct
     6301 gattcacgcg attaagcttg cccacagcgg cagccattaa cacttcctgg gtggcctgct
     6361 cacgagtaaa ttccccgctg agatgccctt catgcatgac gatgatgcga tcgctcatgc
     6421 ctaatacttc tggcatctcc gatgacacca gaatgatgct caagccatcg gctttgaact
     6481 ggttaatcag ttgatagatc tcttttttcg cgccgacatc tacgccacgg gtaggttcat
     6541 caaggatcaa cactttgggg cgtgtcatca gaccgcgggc aatcgccact ttttgctgat
     6601 tgccaccgga aagcagacca attgcctgtt ccatcgacgg tgttttcaca ttaaacagac
     6661 gaatgaaatc actcacagcc tgctgttcat cggcatgctt caaactgccg ccagcgcggc
     6721 tgaagtagcg cagcgctgtc agcgacatgt tctcttttac tgacatgccc aacactaaac
     6781 cgtcacgttt acggtcttcg gagatataca caatgccgtt tgccaggcca tcctgcggtg
     6841 aacgggtaac gacttcatgc ccatccaggg tgacgtaacc gctggtgcgc ggtagtgcgc
     6901 cgtagagcac tttcatcagt tcggtacgac ctgcgcccat caaaccagag acgccaagaa
     6961 tttcgccttt gcgtaaagta aaagagacat cgttaacgcc aggtccgcag agattatcga
     7021 ctttcaggcg gatatctccc ggcgctttgc ccaggtgcgg atattgatct tccagcttgc
     7081 gacccaccat catctcaatc agcgaatctt cggtcagtga tgccacttcg cgctcagcaa
     7141 taaattgccc gtcacgaaaa acggtaacgt catcgcaaat ctcgaagatt tctttcatgc
     7201 ggtgggagat atagacaata ccgcggcctt gcgattttag ttcgcggatg acgcggaaca
     7261 gggattcggt ttcggtatcg gtcagcgcat cggtcggttc atccataatg atgactttcg
     7321 actcaaagct cagcactttg gcgatttcaa ccatttgctg gtcaccgatg gaaagatcgc
     7381 ccaccagctt gtcgctttta aagcgcaggt taagtttagc cagcaattta tccgcttcgg
     7441 catacatggt tttccagtca attttgccaa agcgattaac aaactcacga ccgaggaaaa
     7501 tgttttcggc aatggtcaac tgcgggatca ggttcagttc ctgatggata atcccaatcc
     7561 cggcttcctg ggaagatttc ggcccggtaa atgtcgtttc tttccccagc cataaaagcg
     7621 tgccggcatc gcgagcatag ataccagtaa gcactttcat catggtggat ttacccgcgc
     7681 cgttttcgcc caccagcgcc atcacgcggc ccggatagac atttaacgct gcgcccgaga
     7741 gggcttttac gcccgggaag gctttatcga tgcctttaag ctgaagtaat gcttccatga
     7801 cggcctcaga acgtcacgcc agcacagaga atgatattcg catacggaga acattctccg
     7861 ctgcgaatta ccgcctgact ttctgcggtt tgttgtttga attgttcatg cgtggtgtaa
     7921 cgaatttcaa tggtatttcc ctggtgtttt tgcagctgct caaggtgagt gagcaacgtt
     7981 tcgtggagtt gcggattatg ttgtttgatc tcttccgcga taatggcagc ctcgacctgc
     8041 atttcatttg taacgacgcc cagcacctgc ataaaagaag gtacaccctg ggttaatgcc
     8101 atatcgatac gcgttgtact tttggagatg ggtaaaccag catcacacac caccagcgta
     8161 tcggtatgtc ccagacggga gatcaccgat gaaatatcag aattaagaac ggtgcctttt
     8221 ttcatttttt tctccatcag cgaaacgttt cgctgaccca cagtttaatc ctgactgagt
     8281 tgggaaaacc atcgtgacgt tacggaaatg tgatcaacct cgaaacgttt tacatggtga
     8341 ttaaccatga aaacaaaaac gcccccttat ggagaaagga ggcgtctggc gttagatttc
     8401 gacctgagta cccagttcga taaccctgtt tggcgggatt tcaaattgat ctggtgcacg
     8461 cagcgcatta cgttgcagca gcaagtacag cttgccgcgc agacgcaaat accacgggcg
     8521 tttgccgagg atcaacgact catgcgacat aaagaaggag gtttccatca tccggcaact
     8581 taatccttcc agaccgcagc ggtggaaaac ttcttccacg tttggcgttt ctcgccaacc
     8641 ataacttgcc accacgcgcc agaaagtggg cgacagttgt tcaatctgta cccgacggac
     8701 attatggaca tatggcgcgt cttcggtacg cagagttaac agaatcaccc gctcatgcaa
     8761 taccttgtta tgtttaaggt tatgcatcag cgcaaaggga atgacgttga ttgcacgcga
     8821 catatacacc gcggtcccgg gcacgcgaac aggcggtgat ttctccagcg aagcaatcat
     8881 cgcttccaga gagttaccat gttcatgcat ccgccgcagc aagcggaaac gctcgctctt
     8941 ccaggtggtc atcacgataa acatcaccgt accgaggctc aatggcaacc agccgccgga
     9001 gagcagttta tcgaggttag cggtgaacaa tggaatatcg acacaaagga aagcaatcag
     9061 gatcagcgca acaaaatact tattccagtg ccagttctga cgtgccacgg tagtcgagag
     9121 aatagacgtc agcaccatgg ttccggtcac cgcaatcccg tacgccgccg ccaggttgct
     9181 ggagtgctca aagctgacaa tcacaatcac gaccgcgaca tagagcatcc agttcacaaa
     9241 cggaatatag atttgccctg actccatttc ggaggtgtga ataatgcgca tcggcgataa
     9301 atatcccaga cgtaccgcct gacgcgtcaa tgagaagacg ccagagataa ccgcctgcga
     9361 ggcaattacc gtcgccagtg cggcgatgat cagcagcggg atcagcgccc agtccggtgc
     9421 caacaggaag aacgggttct taatcgcttc cgggttcttt aacaacagcg ctccctgacc
     9481 gaagtaatta agggtcaagg aaggtaatac gacggtgaac cacgccaggc gaatagggaa
     9541 cttaccaaag tgccccatat cagcatacag cgcctcgacc cccgtaatcg acaacaccac
     9601 tgcccctaat gcaataaaag aaaccgtttt gtattcaagg aagaaatgca ccgcccacat
     9661 tggattcagc gcatgcagca cttccgggtt agcaataatg ctacgtaacc ccagtcctgc
     9721 cagaatcaaa aaccaggtca gcatgatcgg cgcaaacagc ttaccgacca tagccgtgcc
     9781 atgtttttga atcataaata ataaagtgag aacgataatt gagaggggaa ctatccaggt
     9841 atccagctgc ggggcgacga tttccagacc ttcaatggct gacatcaccg aaatagcggg
     9901 tgttatgacg acttcaccat agaaaaagct gccgccgatt agccccataa tcaccagcat
     9961 tgatgtggtt cgcgccgacg tattacgccc ggcaagcgac atcaacgtca ggatcccccc
    10021 ctcaccggcg ttatctgccc gcatcacgaa ggtgagatat ttaatggaaa ccacaaagat
    10081 tagcagccag aagatcagcg ataaaaagcc aaacacggca tcgcgttcaa cgccaaaacc
    10141 aaactggccg gacaaacatt cacgaagtgt atataacggg ctggtaccaa tatcgccgta
    10201 gacaactcca atcgccgcga gggtaatcgc gggcaatgat tgcttattat cagtgctcat
    10261 agactagtct ttcgttgaaa tatgaaatgt gtgcttagtc ccttggccca caaaaaagcg
    10321 cacagtatgc acgattaacg gcaaaatcgt actcctaaat gcggccacat taacctggct
    10381 caaagaaaaa taccccccct ttgagactat tttagccacc agcaagaaac gtctatactc
    10441 gcaatttacg cagaactttt gacgaaagga cgccacttca ttatggctca ccctcattta
    10501 ttagcggaaa gaatttcccg cctgagcagt tcgttggaaa aggggcttta tgaacgtagc
    10561 cacgccatcc gcttgtgttt attagcggca ttaagtggtg aaagtgtgtt cctccttggc
    10621 ccgccaggta ttgccaaaag tttgatcgcc cggcgcttaa aattcgcctt tcagaatgcc
    10681 cgcgcgtttg aatatctgat gacccgcttc tccacgccgg aagaagtttt tggtcccctt
    10741 tctattcagg cgctaaaaga tgaagggcgc tatgaacgtt taaccagcgg ttacctgccg
    10801 gaagcagaaa ttgtctttct ggatgagatc tggaaagcgg gcccggcaat tcttaatacc
    10861 ttgctcaccg ccattaacga gcgccagttc cgcaacggtg cacacgtaga aaaaatcccg
    10921 atgcgtctgc tggtggcggc ctccaacgag ctgccggaag cagacagcag tctggaagcg
    10981 ttatatgacc gcatgctgat tcgtctgtgg ttagataaag tgcaggataa agcgaatttc
    11041 cgctccatgc tgaccagtca acaggatgaa aacgacaatc cggttcctga tgccttgcag
    11101 gtcacagatg aagaatatga acgctggcag aaagagattg gtgaaattac gctgcccgat
    11161 catgtatttg agctgatttt tatgctgcgc cagcaactgg ataaattacc ggatgcgcct
    11221 tatgtctcgg atcgtcgctg gaaaaaagcg atccgattat tgcaggccag cgcctttttt
    11281 agcggtcgca gtgctgttgc cccggtagat ctcattttgc tgaaagattg cctgtggtat
    11341 gacgcgcaaa gcctgaattt gatacaacaa caaattgatg tattgatgac cggtcacgcc
    11401 tggcaacagc aagggatgtt gactcgcctt ggcgcgattg tgcaacgtca cctgcaactc
    11461 cagcagcaac aaagcgataa aacagcctta acggtaattc gtctgggcgg cattttcagc
    11521 cgtcgtcagc agtatcaact tcctgttaac gttactgctt ccactctgac tctactgctg
    11581 caaaaaccgt taaaactgca tgatatggaa gtggttcata tctcctttga gcgtagcgcg
    11641 ctggaacagt ggctgagcaa aggtggtgaa attcgcggta aactgaacgg tatcggcttt
    11701 gcccagaaac tgaatctgga agttgatagc gcccaacatc ttgtcgttcg cgatgtaagt
    11761 ttacaaggca gtacgctggc acttcccggt tcatcggctg aaggtctgcc tggtgaaata
    11821 aaacaacaac tggaagagct tgaaagcgac tggcgcaagc aacacgcttt attcagcgaa
    11881 cagcaaaaat gtctgtttat ccctggcgac tggttaggtc gcattgaagc cagcctacag
    11941 gatgtcggtg cacagattcg ccaggcacaa caatgctaac gctggatacg cttaatgtga
    12001 tgctggccgt cagcgaagag ggattgatcg aagagatgat catcgcactg ctggcctcac
    12061 cgcagctggc ggtcttcttt gaaaaattcc cacgcctgaa ggcggcaatc actgatgatg
    12121 ttccccgctg gcgtgaagcg ctgcgcagtc ggctgaaaga tgcccgagtc ccgccggaac
    12181 tcaccgaaga ggtgatgtgc tatcagcaaa gccagctcct ctccacgcca cagtttattg
    12241 tgcagctacc acagatcctg gacttactgc atcgtctgaa ttctccatgg gcagaacaag
    12301 cccgacagtt ggttgatgct aacagcacga tcacttcagc gttacacacg ctttttctcc
    12361 agcgttggcg tttaagtctg atcgtgcaag caacgacgtt aaatcaacag ctattagaag
    12421 aagaacgcga acaactgttg agtgaagttc aggaacgcat gacgctgagc ggacaacttg
    12481 aaccgattct cgcagataac aatactgcag ctggtcgtct gtgggatatg agcgccggtc
    12541 agcttaaacg tggcgactat cagttgattg tgaaatacgg tgaatttctt aacgaacagc
    12601 cggaactgaa acgcctggca gagcagctgg ggcgttctcg ggaagccaaa tcaataccgc
    12661 gcaacgatgc gcagatggaa accttccgca ccatggtgcg cgaaccggcg acggttcctg
    12721 agcaggttga tggtctgcaa caaagcgatg atattttacg tctcctgccg ccagaactgg
    12781 cgacactagg gataacggaa ctggagtatg agttttaccg tcggctggtg gaaaaacagt
    12841 tgctcaccta tcgcctgcac ggtgagtcgt ggcgtgaaaa agtgatcgaa cgtccggtgg
    12901 tacataaaga ttacgatgaa cagccgcgcg ggccgtttat tgtctgtgtg gatacttccg
    12961 gctcaatggg cggctttaat gaacagtgtg cgaaagcgtt ctgcctggcc ttgatgcgca
    13021 ttgctctcgc agaaaaccgg cgctgctata ttatgctatt ttccaccgag atcgtccgtt
    13081 atgagctttc aggcccacaa ggcatcgaac aagcaatccg ttttttaagc cagcagtttc
    13141 gtggcggcac cgatcttgcc agttgttttc gcgccattat ggaacgcttg caaagcaggg
    13201 aatggtttga tgccgatgcg gtggtgattt ctgattttat cgctcagcgg ttgcctgacg
    13261 acgtgacgag taaagtgaaa gagctgcagc gggtacatca gcatcgcttt catgccgtgg
    13321 cgatgtcggc acacggcaaa cccggcatca tgcgcatttt cgatcatatc tggcgctttg
    13381 ataccgggat gcgaagccgc ctgctcagac gctggcggcg ataaattatt acagcagaga
    13441 agggacgctc tcgcgaacag cagctggcca tactccacac tgaacctggc cgatatgcgg
    13501 cagttgcagc agcagcatag tcaaacgaga ctggccgata ccgccgccga tggtctgcgg
    13561 catttcaccg cgcagcagcg cctgatgcca ttccagctgc agacgatctt catcaccggt
    13621 cagcgccaac tgatgcttca acgtgtcggc atccacgcgg atccccatgg aagaaagctc
    13681 aaacgcatct tccagtacag ggttccacac cagaatatcg ccgttcagac ccgcatgacc
    13741 cagctctgac ggggtgctcc agtcatcata atccggtgcg cgcacgtcgt ggcgatgacc
    13801 atcgctcagc ttgccgccaa tcccgacaag gaataccgcg ccaagatctt tcgctatcgc
    13861 ccgctcacgc cctttggcat caagatccgg ataacgagac agtaactcct ggctgtgtac
    13921 gaagtggatc tgatccggca ggaacggtgc caggccaaac tcttcgctaa ccgcagcttc
    13981 ggttgcttta attcccgccc agatcgcctc taccgtgctt ttcagagtcg agaattgacg
    14041 ctcaccgtcg cccattacgc gttcccagtc ccactggtca acatagaccg agtgcaacgg
    14101 agaaagacgg tcttcatcgg ggcgaagggc tttcatgtgc gtgtacagcc cttcgcccgc
    14161 gctgaagtcg tgttgcccta aggtctgacg tttccacttc gccagtgaat gaaccacttc
    14221 gaactgggca tcaggcagaa ctttcacttt tacctgcacc gctttttcac agcccgacaa
    14281 gttatcctgc gtgccatccc ccacacggct aagaatcggc gcctggactt cgatcagccc
    14341 cagacgttct tccagttggc gagaaaagtg agatttcacg aagctaattt ggcgttgttt
    14401 ggcaatgtaa gcggttttca ttttttatac tcctgcgtcc tgttgcttat gattaagcaa
    14461 caaaaaccga cacacatgca ataatcattc aataaaaagc ctaacacact tttgattcat
    14521 taaaaaagaa ggctaaaata caatgaatca tcaatctgca taagaaaatc ctatggaaaa
    14581 ttatctgatc gacaatctgg accgtggcat cctggaagca ttaatgggca atgcgcgcac
    14641 cgcttacgcc gaactggcga aacaatttgg cgtcagtccg gggacgattc acgttcgggt
    14701 agagaaaatg aagcaggcgg ggatcattac cggggcgcgt attgatgtca gcccgaagca
    14761 gctcggttat gacgtaggct gctttatcgg cattatatta aagagcgcca aagactaccc
    14821 ttccgcgctg gcaaagctgg aaagccttga tgaagtcact gaagcctatt acacaacagg
    14881 ccactacagc atctttataa aagtaatgtg ccgttcgatc gacgctctcc agcatgtact
    14941 tatcaacaag atccaaacaa ttgatgaaat tcagtccacc gagacattga tcgtcctgca
    15001 aaacccgatc atgcgtacta tcaagccctg atcggctttt ttaatcccat acttttccac
    15061 aggtagatcc caacgcgttc acagcgtaca atacgccact cttaataaag gtggcggttt
    15121 atggcagata tcactcttat cagcggcagc accctcggcg gtgccgaata tgtagcagaa
    15181 cacctggctg aaaagctgga agaggcgggt tttaccaccg aaacgctgca cggtccgctg
    15241 ttggaagatt tatctgcctc ggggatctgg ctgattatca gctccaccca cggtgccgga
    15301 gatattccgg acaacctttc tcctttctat gaagcattgc aggaacagaa gcccgatctt
    15361 tctgcggtcc gctttggcgc aatcggtatt ggtagtcgtg aatatgacac cttttgtggg
    15421 gctatcgata aactcgaggc agaactcaaa gattccggtg caaaacagac aggcgaaaca
    15481 ctgaagatca acattcttga tcacgacatt ccggaagatc cggcagaaga atggctggga
    15541 tcgtggatta atttactcaa ataagtatac agatcgtgcg atctactgtg gataactctg
    15601 tcaggaagct tggatcaacc ggtagttatc caaggaacaa cggttgttca gtttttgagt
    15661 tgtgtataac ccctcattct gatcccagct tatacggtcc aggatcaccg atcattcaca
    15721 gttaatgatc ctttccaggt tgttgatctt aaaagccgga tccttgttat ccacagggca
    15781 gtgcgatcct aataagagat cacaatagaa cagatctcta aataaataga tcttcttttt
    15841 aatacccaag atcccaggtc tttctcaagc cgacaaagtt gagtagaatc cacggcccgg
    15901 gcttcaatcc attttcatac cgcgttatgc gaggcaatca ccatgtttta tccggatcct
    15961 tttgacgtca tcatcattgg cgggggtcat gcaggcaccg aggccgcgat ggccgcggcg
    16021 cgtatgggtc aacagactct gcttttgaca cacaatatcg acactctggg gcagatgagc
    16081 tgcaacccgg cgatcggcgg tattgggaag ggacatctgg taaaagaagt ggatgcactc
    16141 ggcggtctga tggcgaaagc gatcgatcaa gcgggtatcc agtttaggat actaaacgca
    16201 agcaaaggac cggcggttcg cgctacccga gctcaggcgg atcgtgtgct ctaccgtcag
    16261 gcggtacgta cggcgctgga gaaccaaccg aacctgatga tcttccagca ggcggttgaa
    16321 gatcttattg tcgaaaacga tcgcgtggtc ggtgctgtta cccaaatggg actgaagttc
    16381 cgtgccaaag ccgtcgtgct caccgttggg acgttcctcg acggtaaaat tcatatcggt
    16441 ctggataatt acagcggtgg ccgtgctggt gatccgccgt ccattccgct ttctcgccgt
    16501 ttgcgtgaac tgccgctgcg cgttggtcgt ctgaaaaccg ggacaccacc gcgtattgat
    16561 gctcgaacta tcgactttag tgtgctggcg caacagcatg gcgataaccc aatgccggta
    16621 ttctcgttta tgggcaatgc gtcccagcat ccccagcagg tgccgtgtta tatcactcat
    16681 accaacgaga aaacccatga tgtgatccgc agtaacctcg atcgtagccc aatgtatgca
    16741 ggggtgatcg aaggtgtcgg cccacgctac tgcccgtcga tcgaagacaa agtcatgcgc
    16801 ttcgccgaca gaaatcagca tcagatcttc cttgaaccgg aaggactgac ctctaacgaa
    16861 atttatccga acggtatctc caccagcctg ccgttcgatg tgcagatgca aatcgtccgc
    16921 tctatgcagg ggatggaaaa cgcgaagatc gtgcgtccgg gttatgccat tgagtatgac
    16981 ttcttcgatc ctcgcgacct gaaaccgacg ctggagagca agtttatcca ggggctgttc
    17041 tttgctggtc agattaacgg cactaccggt tacgaagaag ccgctgcgca aggtttactg
    17101 gccggtctta acgctgcccg tctgtctgct gacaaagaag gttgggctcc ggcgcgttct
    17161 caggcgtatc tcggcgtact ggttgatgac ctgtgcactt taggaaccaa agaaccgtat
    17221 cgtatgttta cctcgcgcgc agaatatcgt ctgatgctgc gcgaagataa tgcggatctg
    17281 cgtttgactg aaatcggtcg tgaactgggc ctggtggatg acgaacgttg ggcgcgcttt
    17341 aacgagaaac ttgagaatat cgagcgtgag cgtcagcgtc tgaaatcgac ctgggtaacc
    17401 ccgtcggcgg aagctgcagc cgaagtgaat gctcacctga ctgcgccgct ttcccgtgaa
    17461 gccagtggtg aagatctgct gcgtcgtccg gaaatgactt atgaaaaatt aaccacgctg
    17521 acgccgtttg cccctgcgtt gacagacgaa caggcggcgg aacaggttga gattcaggtt
    17581 aaatacgaag gttatatcgc gcgccagcaa gatgagatcg aaaagcagct gcgtaacgag
    17641 aacaccctgc tacccgcgac actggattac cgccaggtat ccggtctttc taacgaagtg
    17701 atcgccaaac ttaacgatca caaaccagcc tctatcggcc aggcttcgcg tatttctggc
    17761 gtcacgcctg cggccatctc cattctgctg gtgtggctga aaaaacaggg tatgctgcgt
    17821 cgtagcgcat aacgcattaa aaatgcctgg taagcacccg cttaccaggc aacgcatcaa
    17881 gaacaggtaa tcaccgtgct caacaaactc tccttactgc tgaaagacgc aggtatttcg
    17941 cttaccgatc accagaaaaa ccagcttatt gcctacgtga atatgctgca taaatggaac
    18001 aaagcgtaca acctgacttc ggtccgcgat cctaatgaga tgctggtacg ccatattctc
    18061 gatagcattg tggtggcacc gtatctgcaa ggtgaacggt ttatcgatgt cggcaccgga
    18121 ccaggactgc caggcattcc actctctatc gtgcgtcctg aagcccattt cactctgttg
    18181 gatagccttg gtaaacgcgt gcgtttcctt cgtcaggtgc aacatgagct taaactggag
    18241 aatattgaac cagtacagag cagggtagaa gagtttcctt cagagccgcc atttgatggc
    18301 gtaattagcc gcgcttttgc ctctctgaac gatatggtga gctggtgcca ccatcttcct
    18361 ggtgagcaag gccgtttcta cgcgctgaaa gggcaaatgc cggaagatga aatcgctttg
    18421 ttgcccgaag aatatcaggt cgaatcagtg gttaaacttc aggttccagc cctggatggc
    18481 gaacgtcatc tggtggtgat taaagcaaat aaaatttaat ttttatcaaa aaaatcataa
    18541 aaaattgacc ggttagactg ttaacaacaa ccaggttttc tactgatata actggttaca
    18601 tttaacgcca cgttcactct tttgcatcaa caagataacg tggctttttt tggtaagcag
    18661 aaaataag
//
LOCUS       DBANUU010000087        17820 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000087 DBANUU010000000
VERSION     DBANUU010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.087"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..581
                     /locus_tag="ACIE68_003071"
     CDS             <1..581
                     /locus_tag="ACIE68_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HFX5909347.1"
                     /translation="WSNTSFGIHRFKVKLDTTYCIKYAILAFLALLPFLAVAGYIIFD
                     QILNAYDSSVYANDDIENLQQFMEMQRKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSN
                     LSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLKIWMIDWQAKNTYLLGD
                     LDDLPLINKEEQPDKGFLASISRGIMPSLPFL"
     gene            complement(635..3490)
                     /gene="valS"
                     /locus_tag="ACIE68_003072"
     CDS             complement(635..3490)
                     /gene="valS"
                     /locus_tag="ACIE68_003072"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFX5909348.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(3490..3933)
                     /gene="holC"
                     /locus_tag="ACIE68_003073"
     CDS             complement(3490..3933)
                     /gene="holC"
                     /locus_tag="ACIE68_003073"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFX5909349.1"
                     /translation="MKNATFYLLDNDTTIDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(4287..5798)
                     /gene="pepA"
                     /locus_tag="ACIE68_003074"
     CDS             complement(4287..5798)
                     /gene="pepA"
                     /locus_tag="ACIE68_003074"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFX5909350.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            5952..6002
                     /gene="ytgA"
                     /locus_tag="ACIE68_003075"
     CDS             5952..6002
                     /gene="ytgA"
                     /locus_tag="ACIE68_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFX5909351.1"
                     /translation="MSIIILATISTTQENK"
     gene            6065..7165
                     /gene="lptF"
                     /locus_tag="ACIE68_003076"
     CDS             6065..7165
                     /gene="lptF"
                     /locus_tag="ACIE68_003076"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFX5909352.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            7165..8247
                     /gene="lptG"
                     /locus_tag="ACIE68_003077"
     CDS             7165..8247
                     /gene="lptG"
                     /locus_tag="ACIE68_003077"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFX5909353.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(8408..9910)
                     /gene="yjgR"
                     /locus_tag="ACIE68_003078"
     CDS             complement(8408..9910)
                     /gene="yjgR"
                     /locus_tag="ACIE68_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFX5909354.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAQAGTVSEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(9988..10986)
                     /gene="idnR"
                     /locus_tag="ACIE68_003079"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(9988..10986)
                     /gene="idnR"
                     /locus_tag="ACIE68_003079"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFX5909355.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            complement(11053..12372)
                     /gene="idnT"
                     /locus_tag="ACIE68_003080"
     CDS             complement(11053..12372)
                     /gene="idnT"
                     /locus_tag="ACIE68_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFX5909356.1"
                     /translation="MPLIIIAAGVALLLILMIVFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGHYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(12435..13199)
                     /gene="idnO"
                     /locus_tag="ACIE68_003081"
     CDS             complement(12435..13199)
                     /gene="idnO"
                     /locus_tag="ACIE68_003081"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFX5909357.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(13223..14254)
                     /gene="idnD"
                     /locus_tag="ACIE68_003082"
     CDS             complement(13223..14254)
                     /gene="idnD"
                     /locus_tag="ACIE68_003082"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFX5909358.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLRGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            14471..15034
                     /gene="idnK"
                     /locus_tag="ACIE68_003083"
     CDS             14471..15034
                     /gene="idnK"
                     /locus_tag="ACIE68_003083"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFX5909359.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            complement(15038..16057)
                     /gene="ahr"
                     /locus_tag="ACIE68_003084"
     CDS             complement(15038..16057)
                     /gene="ahr"
                     /locus_tag="ACIE68_003084"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFX5909360.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADY"
     gene            16253..16337
                     /locus_tag="ACIE68_003085"
     tRNA            16253..16337
                     /locus_tag="ACIE68_003085"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16287..16289,aa:Leu,seq:caa)
     gene            16501..17757
                     /locus_tag="ACIE68_003086"
     CDS             16501..17757
                     /locus_tag="ACIE68_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFX5909361.1"
                     /translation="MKLNARLVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPTVTLAQARAKRDEAKRLLVQGIDPKAEQKAAQAEKSGAYTFET
                     IAREWHASNKRWSEDHRARVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTVKTRHYPALPSHRFPELLARL
                     AAYRGRIMTKIAVQLSLLTFVRSSELRFARWREFDFDNAIWRIPAKREEIKGVRYSYR
                     GMKMKEEHIVPLSHQAMELLRQLTQISGDKEFLFPGDHDATKVMSENTVNNALRSMGY
                     DTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHAERNNVRAAYIHTSEHLDERRL
                     MIQWWADYLGANKEKYITPYDYAKSE"
BASE COUNT         4311 a         4620 c         4438 g         4451 t
ORIGIN      
        1 tctggagcaa taccagtttc ggtatacatc gtttcaaggt gaaattagat actacgtatt
       61 gtataaaata tgccattctc gcatttttag ctttattacc ttttctcgct gttgctggtt
      121 atattatctt cgatcaaata ttaaatgcgt atgatagttc tgtgtatgca aatgatgata
      181 ttgagaattt acagcaattt atggaaatgc aacgtaaaat gataatcgcg cagttaatct
      241 attattttgg gattgctgtt agcaccagtt atttaacggt gtcgttgcga aatcatttta
      301 tgagcaacct gtcactgaat gatgggcgta ttcgttttcg ctcaacttta acataccacg
      361 gtatgcttta tcgcatgtgt gcgttggtgg tgatatccgg gattacgggc ggtctggctt
      421 atccactgct gaaaatatgg atgattgact ggcaggcaaa aaatacgtat ttgctgggcg
      481 atttggatga ccttccttta atcaataaag aagaacaacc agataaaggc ttcttagcca
      541 gtatttcacg gggaattatg ccttctttac catttctgta agagataaaa aaggccggag
      601 catgctccgg ccttcgtttt catcacggtg ttgattacag cgcggcgata acagcctgct
      661 gttcaatcag cttcgctttc gcttccgcat agccttccag cttctcacgc tctttcgcga
      721 tgaccgcttc cggtgcgcgg gcgacaaagc cttcattcgc cagcttgttc tcgatacggc
      781 tgatttcgcc ttcaatcttc gccacttctt tcgccagacg cgccagctca tcttctttgt
      841 tgatgaggcc agccatcggg atcagcagct ctgcgccgtc gataatcttc gtaacggaaa
      901 ccggaccttt gtcatcggca ggcagcacgg tgatgctttc cagacgcgcc agggtttgca
      961 ggaagccacg gttttcattt acgcgacgtt ctgcatccgc gctgcaacca cgcagcagca
     1021 gctccagcgg tttgcccggc gcgatgttca tttctgcacg gatgttacgt accgcaacga
     1081 tcgcctgctt cagccactcg gtgtcggcca gtgcggcttc atcaacctga gatgcatcgt
     1141 actgcgggaa cggctgcagc atgatggtgt cggcagtgat accgcaaagt actttcacac
     1201 gctgccagat ggtttcggtg atgaacggaa tgatcggatg cgcgaggcgc agcagacctt
     1261 ccagtacagt caccagcgta tggcgagtac cgcgcagttc tgcttcggtg ccaccgttca
     1321 ttaccggctt ggtcagctcg agataccagt cacagaactg gttccaggtg aactcataca
     1381 gaatgcctgc ggcgatatcg aagcggaagc tgtccagcgc ttcgcggtac gctttgatgg
     1441 tctggttgaa ctccgccaga atccagcggt ccgccagcga cagcgtcatt tcgccgccgt
     1501 tgaagccgca atcctgacct tctgtgttca tcagcacaaa gcggctggcg ttccacagct
     1561 tgttacagaa gttacggtaa ccttccaggc gcttcatatc ccagttgatg tcacgaccgg
     1621 tagacgccag tgccgccagg gtgaagcgca gcgcgtcagt accgtgcggt tcaataccgt
     1681 tcgggaactg cttctcggtg cgcttacgga ttttgtccgc cagctgtggc tgcatcatat
     1741 tgccggtgcg tttttccagc agttctggca gcgaaatacc gtcaaccata tccagtgggt
     1801 cgataacgtt acccttggat ttggacatct tctggccttc gtcgtcacga atcagaccgg
     1861 tcatgtaaac agtgtggaac ggcacctgcg gtttgccatt ttcatctttg atgaagtgca
     1921 tggtcatcat gatcatgcgg gcaatccaga agaagatgat gtcgaaacca gataccatca
     1981 cgctggttgg gtggaactga cgcagggcgt cggtgttttc cggccagcca agggtagaga
     2041 aggtccacag cgcagaagag aaccaggtgt cgagaacgtc ttcgtcctga cgcaggacaa
     2101 catcagcacc gaggttattt tctttacgca cttcgtcttc gttgcggcca acataaacgt
     2161 tacccgcttc gtcataccat gccgggatac ggtgacccca ccacaactga cgagagatac
     2221 accagtcctg aatatcgcgc atccaggaga agtacatgtt ttcgtactgc ttcggtacga
     2281 actgaatgtc gccgttctca accgcttcaa ctgccggttt cgccaggaca tcggcacgca
     2341 cgtaccactg gtcggtcagc atcggttcga tgaccacgcc accacggtcg ccataaggaa
     2401 cggtcaggtc atgtggttta atttcttcca gcaggccgag tgcgtcaacc gcagcaacga
     2461 ccgctttacg tgcagcaaaa cgctccagtt tctggaattc tgcggggatc tcgctggaat
     2521 aaacgtcaga ttcgttacct ttggtatcga acacctgggc actttcacgg atatcgccgt
     2581 caaaggtcag gatgttgatc atcggcagcg cgtgacgttt acccacttca tagtcgttaa
     2641 agtcgtgcgc cggagtgatt ttcacgcagc cggtgccttt ttccatgtcg gcgtgttcgt
     2701 cgccaacgat cggaatacga cggttaacca gcggcagaat gacatatttg ccaatcagat
     2761 ctttgtaacg cggatcttcc gggttaacgg ctacgccagt atcgcccagc agggtttccg
     2821 gacgggtagt cgcaaccacc agataatctt taccgtctgc ggttttcgcg ccgtcagcca
     2881 gcggatagcg gatgtgccac atcgaacctt tcgattcgcg gttttccact tccaggtcag
     2941 agatagcggt gcgcagtttc gggtcccagt ttaccaggcg cttgccacgg taaatcaggt
     3001 cttctttata cagacgaacg aaaacttctt tcaccgcatt ggacaggcct tcgtccatgg
     3061 tgaagcgttc acgctcccag tccacggagt tgccgagacg gcgcatctga cgggtaatgg
     3121 tgccgccgga ttccgctttc cattcccaga ttttgtcgat gaaagcttcg cggccgtagt
     3181 cgtgacgggt tttaccttct tctgcggcaa tcttgcgctc aacgaccatc tgggtagcga
     3241 tcccggcgtg gtcagtaccg acctgccaca gggtgttttt gccctgcatg cgctgatagc
     3301 ggatcatggt atccatgatg gtttgctgga aggcgtgacc catatgcaaa ctgccggtga
     3361 cgttcggcgg cgggatcatg atgcagaaac tttcctggct ttcatcgcca ttaggcttaa
     3421 agtagccctg cttttcccag tgctcgtaaa gcggctgttc gatatcttgt gggttatatg
     3481 tcttttccat tatttccagg ttgccgtatt caggttgaaa ccagccacgc ggtaggcttt
     3541 atagcgttcg cgcgccagtt gtttcagaga atcttcataa ggaacgaagt ctaccacttc
     3601 tgtgaaagcg gtggcaaaat ctgcaaagct tgttcgcaga ctaatcaata tatcgcgcgg
     3661 gctgctgctg cgtttttgcg gccaggcgat ctccaccggc gcaccgccgc gcggtccttc
     3721 tcccgctaaa ttatgcggaa caaagctttc tgccggacgc gcccacaggg cttcatccag
     3781 ccggtaagcc tgcttttcat cttcacaggc gatgagcacg cgcttaccgc tgcgccaacg
     3841 ttctgcggca atttcacaca ccagctgctc aacggcgctt aagccatcga tggtggtgtc
     3901 attgtccaga aggtagaacg ttgcgttttt catatatggg gcttcttgtg gtggatttaa
     3961 atacgcatca ggtatacacc gcagcgcctg atgcgacgct gttgtgtctt atcaggccta
     4021 cgagttcagt gctgtgtgta ggtcggataa ggcgctcacg ccgcatccga caataacagc
     4081 attgcctgat gcgacgctgc cgcgtcttat caggcctacg agttcggtgc tgtgtaggtc
     4141 ggataaggcg ttcatgccgc atccgacaat aacagcattg cctgatgcga cgcttgcgcg
     4201 tcttatcagg cctacgagtt cagtgctgtg taggtcggat aaggcgttca cgccgcatcc
     4261 ggcaataaca gccttgcctg acgcaattac tcttcgccgt taaacccagc gcggtttaac
     4321 aggaactgtg ccagcaacgc taccggacga ccggtggcgc cttttgcttt accagaacgc
     4381 caggcggtac cggcgatatc caggtgcgcc cagttgtact tacgggtaaa gcgtgacagg
     4441 aagcaacctg cggtaatcgc cccaccagga cgaccgccaa tgttcgccat atcggcaaaa
     4501 ttggactcca gttgttcctg atactcgtca cccagcggta agcgccatgc gcggtcaccg
     4561 gattgttcag acgcggcaat cagttcatgg gccagcggat tatggttcgc catcagaccg
     4621 gtaatgtgat gacccagcgc gatcacgcag gcaccggtca gcgtcgccac gtcaatcacc
     4681 gcttccggct caaaacgctc aacgtaagtt aacacgtcgc acagtaccag gcggccttca
     4741 gcgtcggtat tcagcacttc aacggtttga ccggacatgg tggttaacac atcgcccgga
     4801 cgataggcac gcccgccagg catgttttcg cagcctgcca acacgccgat aacgttaatc
     4861 ggcagttgta gctccgcgac catccgcatc acgccgtaaa ccgctgccgc accgcacata
     4921 tcgtacttca tctcatccat gccttctgaa ggcttgatcg agataccgcc ggagtcgaag
     4981 gttaaacctt tacccaccag cacgattggg cgtgcatctt ccgacgcgtt gcctttgtac
     5041 tcaatcaccg acatcagcga ttcgttttgc gaaccctgac cgaccgccag ataggaatgc
     5101 atccccagct ctttcatctg ctgttcgccg ataacgcggg tgataacatt cttgctgtag
     5161 ctgtcagcca gctggcgcgc ttgtgaagcg aggtaagcgg cgttacagat attcggcggc
     5221 atattgccga gatctttcgc tgctttaatc ccggcggcaa tcgccagacc gtgctggatc
     5281 gcgcgctcac cgctggtcag ttcacggcgg gtcggcacgt tgaacaccat tttacgcagc
     5341 gggcgacgcg gttcgctctt gttcgttttc agctgatcga aactgtagag cgtctctttt
     5401 gccgtctcga cagcctgacg cactttccag tagttattac ggcctttaac gtgcagttca
     5461 gtcagaaagc agaccgcttc cattgagcca gtatcattca gcgtattaat ggttttctga
     5521 ataacctgct tgtactgacg ctcatccagc tcacgttctt tgccgcaacc aataaggaga
     5581 attcgctcgg aaagtacatt cggaacatgg tgcagcaaca atgtctgccc cggttttcct
     5641 tccagttcgc cccgacgtag cagggcgctg atgtacccat cgctgatttt atcgagctgt
     5701 tctgcaatcg gagaaaggcg acgtggttcg aagacgccca cgacgatgca ggcactccgc
     5761 tgtttctccg ggctaccgct ttttacacta aactccatgc actacgctcc tgaatcttaa
     5821 agacaacggc ggtggctaca gatagaattg caagctttcg taactcatgt ccgctgttgc
     5881 gatgacttcg tgttaatctt aacgttatta cggcattggc acgtcagaac aaaatctgag
     5941 aggtgaatcc gttgagtata attatcttag cgacgatttc gacgactcaa gagaataaat
     6001 gacgtttaag ccatgaaaca agctaaaatc ctgcaaaaga cgagttttta cgggcgtatt
     6061 taaagtgata atcataagat atctggtgcg ggagacgctc aaaagccagc tggcgatact
     6121 cttcatcttg cttttgatct tcttctgtca aaagttagtg aggatcctcg gcgcagcggt
     6181 tgacggcgat attccggcga atctggtgct ctcccttctc gggttgggcg tgccggaaat
     6241 ggcgcagctt atcctgccat taagcctgtt cctcgggctg ctgatgacgc tgggcaaact
     6301 gtataccgaa agtgaaatta cggtaatgca tgcctgcggc ctgagcaaag cggttctggt
     6361 gaaagcggca atgatccttg cggtattcac ggcaatcgtc gcggcggtta acgtgatgtg
     6421 ggcgggaccg tggtcatcgc gtcatcagga tgaagtgtta gcagaagcga aagcgaaccc
     6481 tggcatggcg gcgctggcgc aagggcaatt ccagcaagcg actaatggca gttcggtgct
     6541 gttcatcgaa agcgttgacg gcagcgattt caaagatgtg ttcctcgcgc aaattcgacc
     6601 aaaaggtaat gcacgtcctt ctgtggtggt ggccgattcc ggacatttaa cccagctgcg
     6661 cgacggctcc caggtcgtca ctctcaacca gggaacgcgc ttcgaaggca ctgcactgtt
     6721 acgtgatttc cgcattacgg atttccagga ttatcaggcg atcattggtc accaggcggt
     6781 ggcgctcgac ccgaacgata ccgaccagat ggacatgcgc acattgtgga acactgacac
     6841 cgatcgtgct cgcgcagaac tgaactggcg tatcacgttg gtattcaccg tgtttatgat
     6901 ggcacttatg gtcgtaccgc tgagcgtggt taacccacgt cagggacgcg tactgtcgat
     6961 gctgccagcc atgctgctgt atctactttt cttcctgatc cagacctccc tgaaatcgaa
     7021 cggcggtaaa ggtaagctgg acccgacgct gtggatgtgg accgttaacc tgatttatct
     7081 ggctttagcg attgttctca acctttggga caccgtgccg gtccgccgcc tgcgcgccag
     7141 tttttcgcgt aaaggagcgg tgtgatgcaa ccttttggcg tacttgaccg ctatatcggt
     7201 aaaactattt tcaccaccat catgatgaca ctgttcatgc tggtgtcgct gtcgggcatt
     7261 atcaagtttg tcgatcagct gaaaaaagcc gggcagggga gttacgacgc gttaggcgca
     7321 ggaatgtata ccttgctgag cgtgccgaaa gatgtgcaga tcttcttccc gatggcggct
     7381 ctgcttgggg cgttgcttgg tcttgggatg ctggcgcagc gcagcgaact ggtggtgatg
     7441 caggcttctg gttttacccg tatgcaggtg gcgctgtcgg tgatgaaaac cgccattccg
     7501 ctggtcttgc tgacgatggc gattggcgaa tgggtcgcgc cgcagggcga gcagatggcg
     7561 cgtaactacc gtgcgcaggc gatgtacggc ggctcgttgc tctctaccca gcaaggctta
     7621 tgggcgaaag atggcaacaa cttcgtctac attgagcggg ttaaaggtga cgaagagtta
     7681 ggtggcatca gcatttatgc ctttaacgag aatcgtcgtc tgcaatccgt acgctatgcc
     7741 gctactgcga agtttgaccc ggaacataaa gtctggcgtc tgtcgcaggt tgatgaatct
     7801 gatctgacca atccgaaaca gattaccggt tcgcagacgg tgagcggcac ctggaaaacc
     7861 aacctcacgc cggacaaact gggcgtggtg gcgctggacc cggatgcact ctctatcagc
     7921 ggtttgcaca actatgtgaa gtatctgaag tcgagcggtc aggatgccgg acgttatcag
     7981 ctcaacatgt ggagcaaaat cttccagccg ctatctgtgg cggtgatgat gctgatggcg
     8041 ctgtcgttca tctttggccc actgcgtagc gtaccgatgg gcgtgcgtgt ggtcaccggt
     8101 atcagtttcg gttttgtctt ctacgtactg gaccagatct tcggcccgct gacgttggtt
     8161 tatggcatcc cgccgatcat cggcgcactg ttgccaagcg ccagcttctt cttaatcagc
     8221 ctgtggctgt taatgagaaa atcgtaaccg aatgaacgaa taaagggagc gaaagctccc
     8281 tttattattg ttagcaaagt gtgcttcgtt cattcctgaa aaataattaa aattcaatat
     8341 attgcagtgg ggtgggcatg ataagacgcg ccagcgtcgc atcaggcaaa gacaaatctc
     8401 gcctgactta ccttcttctc ccccccagca aactccccaa catcccgcgt acaatctgat
     8461 tcgtcacttg gcgagcggcg cttttggcca ttgtttgcac cacaccatct ttcttcccgc
     8521 cgcgtggtcc ggtagtgccg aacaaaatat ccttcaatcc accaagaatg ccgtcatcca
     8581 ccgctacctc tttccctttc gcgggaggat tattttgctg ctcggtactg gcctgaaagc
     8641 ctttttgcaa catctcatag gcggattccc ggtccacctc atcctcatat ttgccataca
     8701 ccggagagtg attaatcaag ccattacgct catcttccgt caccggcccc atccgcgaac
     8761 aaggcgcgat caccatcgca cgctccacca cagaagggct tcctttcgca tccagaaaag
     8821 agatcaacgc ctcgccggtg cccagttcct gaatcgcctt ttcggtatca aacgccggat
     8881 tggcccgcat ggtttgcgcc gctgccttca ctgctttctg atcttttggc gtaaaagccc
     8941 gcaaagcatg ttgaacgcga ttaccgagct gcccgagcac attatccgga atatcagacg
     9001 ggttttgcga aacgaaccag acgcccacgc cttttgagcg aataagcctt atcacctgct
     9061 caatcttatc cagcagtacc tgcggggcat cgttaaacag cagatgtgcc tcgtcgaaga
     9121 aaaacaccag ttttggtttc tccagatcgc ccgcttccgg caattgttca tacaactctg
     9181 aaagcatcca cagcaggctg gcggcgtaca gtttcggcat ctgataaagt ttctcggcgc
     9241 tgaggatatt gataacgcct ttaccgttgg catcggtgcg catccagtct ttgatatcca
     9301 gcatcggctc accaaagaag tgtgctgcgc cttgctgttc cagcgacaac agcccgcgct
     9361 ggatggcacc aaccgatgcg ctactgatat taccgtactg attctggaag gatttggcgt
     9421 tatcgccgat gtactgggta attgcccgca gatctttaaa gtcgagcagt aacagcccct
     9481 gatcgtcagc aatgcggaag atgatattca gcacgccaga ttgcacatcg ttgagattca
     9541 acagtcgtgc cagcaacagc ggccccagat ccgaaaccgt cgcccgcacc ggatggcctt
     9601 tctcgccaaa gatatcccac accaccaccg gattggcatg cggttgccag tcattgacgc
     9661 cgatattttt aagccttgcg agcagttttt ccgacaccgt tcctgcctgc gcgacgccgg
     9721 tcagatcgcc tttcacatca gccataaaca ccggcacgcc gatttccgac aatgactccg
     9781 ccagtttttg cagcgtaacg gttttacccg tccccgttgc gccggtaatc agcccatgac
     9841 ggttagccat tcccggcagt aaaaacagtt ctgtgtccgg cgtgcgggca attaacaggg
     9901 gttcactcat gagatatcct ccagtttcct gcctggagta taggcaacgt ccggcaaaga
     9961 gaatacgtgt aatgaagaga aaataaccta aagcgtgttg ccgtgataaa tctgataccc
    10021 gaggtcaaca gtgttgtggt tgtgatcgtt atttttaatt ttgctcagca acatttgggc
    10081 ggccatccga ccaatatcaa aacggggagt aattacgctg gcgaggcttg ggatcatttg
    10141 cctgccaatc tccagcccat gaaaacccgc aatggaaatt tgttctggca cggcgaggtt
    10201 tcgctcgcgg cacagcagaa gcgcacccat cgcaatatcg tcattggtgc aaaatacacc
    10261 gtctaaatcc gggtttgcgc taagggcatc acgcattaac tgcattccta aatgaatgga
    10321 tgagatggcg cgtggattca tgcgtaatgg ggaaagatta tgcagcatca tcgcatcgca
    10381 atacccctga taacgctgtt catcgcgggt gtcatctttg gaaccgagat acagaatttt
    10441 gtgcctgact cgcttctcca gcatggtaca caccatgtca aaagcggcct gccgattatc
    10501 aaaaccgacc tccatatcca gccgttctcc ctgtacatcc atcaattcca cgaccggaat
    10561 ggtggctgaa cgcagaaatt tcaccgtcct gatggtgtga tatttttccg aaagtataat
    10621 cccgtcaatg ttataagaga gcaggttaat caccgactct tcttcggaat cgcgatcgta
    10681 attgtaatta gcaataaggg tctgataatt atgctcagag gtgacggatt ctattcccgc
    10741 gagaatgtca gcgaagagtt gattttgaaa tgacggaatc aagataccga gggtataact
    10801 ttgtgcattc aacaacatgc cgggagcgcg attagggata taattgatct cttccataat
    10861 tttggcgatg cgctcgcctg tttcctttgc cacctttttc ggcgagcgga tataacgact
    10921 cacggtcatt tttgttacgc cagccagcgt agcgatatcc tgtaaagaaa ttctgtgatt
    10981 gcgcataact tctcccaacg tcgggggaat cttcaatctt cccccgaaac tacatcacaa
    11041 tattttattc tttcagtgca atacggcgtt aatggctaac acgcccagca aacccataat
    11101 agaaataagg gtttccatca ccgtccaggt acgcaacgtt tcaccaaccg tcagattaaa
    11161 atacccttta aatagccaga agccagggtc gtttacgtgt gacgcgatca cgctgcccgc
    11221 accggttgcc agtaccatta aagcgggatc ggcatgggta acgttgataa tcggcaacac
    11281 cacacccgcg gtggtaatgg ccgcgacggt ggcagagccc agagcgatac gcaacagcgc
    11341 cgcaacagtc cagcacatca ataacggcga aagtgtagtt ccggtcatta agtgtgaaat
    11401 atagtgcccg acaccgctat ctactaatac ctgcttaaac gcgccgccgc cagcgataat
    11461 aaacacaatc atcgcgatag cgcctataga atccccaatg atatccatga tttgctcgat
    11521 ggtgcgtcca ttacgtcggc ccagtgtgaa aatcgcaata acaatggcaa taaacagcgc
    11581 aacggcaggg ttaccgacaa attcaaagaa gaggcgcacg gtgttagttt tcggtaacgt
    11641 aatttcacaa acggcggcga tagccatcag gatgaccggg atcacggcag cgaaaatact
    11701 gttccagaag gagggcatct cctcttcgct aaacagatga ggattaaata agccttccgg
    11761 tggtgctttc tcaaagcgag ttagcagttt agaaaacagc ggtcctgcga caataactgt
    11821 cggaatggta atgataaatc catacagtaa agtcgttccg agattagcct caaagatagt
    11881 cgcgatggca gtaggccctg gatgtggcgg cagaaaacag tgggttacag agagcgctgc
    11941 taccattggt acgccaacat acaacagggg taatcctgat gatgctacga tggtaaatac
    12001 caacggcaac agcaggacaa aacccacttc aaaaaacatg gcgaggccca caaccagacc
    12061 ggtgatcact agcgcccatt gcacgcgttt tttaccaaaa gtagcaatca gcgtagtggc
    12121 gatacgttgt gccgcacccg tatcagaaat cagcttgcct aacatggccc cgaaaccgag
    12181 gatcattgcc agcccgccga gcgtgctgcc gataccattt tgtatagagt gcaggacggc
    12241 ctgtgcatcc atcccttcgg caaatccgac gacggcagct accagaacga gggcaataaa
    12301 gccgttaact ttaaagacga tcatcaggat aagaagcagc gcgacgcctg ccgcaataat
    12361 gattaatggc atagtcttta tctcttatta gtgccagaaa agcagcgctt tcgcgctgca
    12421 cgtagggggg gctgttaaac agccactaac atgccgccat caacaaacaa caggtggccg
    12481 tttacgaaat cagaggcttt tgaagaaagg aacaccgcag caccaatcag ttcctgcgga
    12541 tctccccagc gtgcggcggg ggtccgtttg cacaaccagg cggtgaaggc ttcgtcctca
    12601 accagtgctt tagtcatttc tgttttgaaa tagcccggcg caataccgtt gacctgaata
    12661 ttgtggcgcg ccagctcgac gcacatgccg cgggtgagca tttttaccgc ccctttcgat
    12721 gcggcataag gggtgatggt gtcacgtccc agttcgcttt gcatcgagca aatattaata
    12781 actttacctg ccttgcgttc aaccatgtga cgagttaccg cttgcgatac caggaacacg
    12841 gcggtctggt ttactgcgat cacatcattc cactcttgtt cagggaactc agtaaaagga
    12901 tgacggcgct ggataccggc gttattcacc agcacatcaa tggggccgat gtccttttcg
    12961 atatgttcaa cggcggcatc aatttcatgt ttatgagtaa cattaaaagg tgcggcaacg
    13021 gcctgaatac cctcctggtg gagtttttct acagcaagtt ctgcgcgttc ggcagtaata
    13081 tcattaataa ttatttgtgc gccatattta cccaggccgg ttgccagtaa aaagccaatg
    13141 ccctgtgctg aaccggtaat caagatattt tttcctgcca gtgaaaatag atcgttcatt
    13201 gtattattcc tttatttatc tcttagaaaa caagctggac ttttgctgcc tgggttttat
    13261 caccggcgaa acgtagcgcc tcttccaggt cagtgaaggg atattcagca ctcagtaaag
    13321 gcagtggatt gataacgcca ttcgccagcc atgacactgc ggtattaaat tcgctggtaa
    13381 aacggaaaga gcctctgagt gaaatctcct taccaatcaa cgtcatcatt gggaattctg
    13441 ccatcgcgcc tcccatacct acctgcacca ttacgccgcg tgcacgagtg acctccagac
    13501 aggtattcac tgatgaagga tgaccggaca cttcaaagct gacatcgaaa tagccttttt
    13561 ccgctttcca gtgatccatg tcgtcgtttt gtgggtttac gagcacatcc gcccccatct
    13621 ctttgcccag cgaaagggaa cggggactca catcagcaca gacaatttcc gcggccccca
    13681 gtgttttcac tgcactgaca atcaggcagc caatgggtcc aacaccggaa ataaatactc
    13741 gcttgccctg taactcgccg gcctgatgtg cggcatgaat cgcgacggct aaaggttcgg
    13801 caaaagccat aaccttttcg tcagctttgg ccggataagg gacacattgc gacgtttcga
    13861 ccattttata acgggtaaaa ccaccatcaa catgagggaa atacatggca ctgccaaaaa
    13921 aacgcatatc tgtacactga ttctcgttat gttcaatgca gtatttgcag tgaccgcacg
    13981 gtttagacgg attaatggct accgtttgcc cttcatgtaa ttctgatgag tcgctatgaa
    14041 taactttacc gataacttca tgacctaaca ccatcggtgc ctttatcatg aaattaccta
    14101 cttttccttc ctgataataa tgtaaatcgg aaccgcaaat tccacctcgg gttatttgta
    14161 ctaatgttcc attattattc caatctatcg tctgctcggt aacggcaaca gttttcttgc
    14221 ccgcaacaac gcaggactgt gttttcactt gcataaattc ctcactggtc aggtagacac
    14281 ctcggaagca tttaagcggt tttaactgtc attatttgtg atgaagatca cgtcagaaaa
    14341 ttgttacatt actatgttac gcataacgtg atgtgccttg taattcttat cagtagaaat
    14401 aaaaaaacgt gaaattatta tgccgccagg cgtagtatcg cagcaggtaa gatgattcag
    14461 gagattttaa atggcgggtg aaagctttat tttgatgggc gtttcaggga gtggtaaaac
    14521 attaattggt agcaaggttg ccgcgttatt atctgctaaa tttattgatg gtgacgatct
    14581 tcatccagcc aaaaatatag ataaaatgtc gcagggtatt ccattatctg atgaagatcg
    14641 acttccctgg ctggaacgct taaatgatgc ttcatacagt ctttataaaa agaatgaaac
    14701 aggatttatt gtctgttcat cattaaaaaa acagtatcgt gatattttac gcaagggtag
    14761 cccccatgtt catttcctct ggttagatgg cgactatgaa actattctcg cgcgaatgca
    14821 gcgtcgggct gggcatttta tgccggtagc gttactaaaa agtcagtttg aggcactgga
    14881 gcgtccacaa gcagatgaac aggatattgt gcgcattgat atcaaccatg atattgccaa
    14941 tgtcaccgaa caatgtcggc aggctgtgct ggcgatacga caaaacagaa tatgtgcgaa
    15001 agagggcagc gcctcagatc agcgctgcga atgattttca ataatcggct ttcaacacca
    15061 cgcggtaacg cgccttaccg tcgcgcacat gctggatggc gtcgttaatt ttcgacatcg
    15121 ggaacagttc ggtggtcggc gcaaccttgc tgcgggcggc aaaacgcatc agcttacgca
    15181 gctcataagg cgtgccggta gcagaaccag agacgctgcg atcgcccgca attaacgtaa
    15241 aggccggaac agacagcggc gtgagaaccg caccgaccgt atggaaatta ccgccatagg
    15301 tcagcgcctc aaaatagggc tgccagtcga ggctgacgtt gacggtgttg ataatgagat
    15361 caaactgccc cgccagtgct ttcagtgcct gcggatcgcg gctattcacc actttatcgg
    15421 cacccatcgc cagcacttcc tgctctttcg ccggattaga actaaaggct gtcacctcgc
    15481 atcccattgc gtgcagaagt tttatagcga tatgccccag cccgccaata ccaattaccc
    15541 caacgcggct ggtagcagtg atatggtgca tcaacagtgg tttaaagacc gtgataccgc
    15601 cgcacaacag cggcccggcg gactcgatat caatattttc tggcagtgga atcacccatt
    15661 gccagtccgc acgcaacttc tcggcaaagc caccgcgatt cataatcgtc ggcaccgcac
    15721 cttgctcgca gttgatctga ttaccgctaa tacaggcgtc gcagtgacca cagctacgcg
    15781 ccgtccagcc aatcccgaca cgctgaccga cctgcaaacc tttatcctgc gcggcgctcc
    15841 cgagtgccac cacgcgccca atcacctcat gcccggcaac cagcggatat tgtgaaaatc
    15901 cccattcgtt atcgatcatc gacagatcgg aatggcagat cccgcagtaa tccacctgca
    15961 cttcaacatc ttgtggcctc agctcaccgg gatcgtactc ataaacttcc agttctccgc
    16021 ccgcttcttt tgcggcatag ctttttatca tcgacatttt ttggtccttc tctggtgttg
    16081 tttgggaagt gtagagcatg gcagggcggg gtgcctggag tgtgacaaag gttacacatc
    16141 gctgtatgca atgctgaaaa tttcagcact tagcgaggtg cgagcaagct ggcgcttgca
    16201 tggtggcgtg cgacaggtat aatccacaac gttttccgca tacctcttca gtgccgaagt
    16261 ggcgaaatcg gtagacgcag ttgattcaaa atcaaccgta gaaatacgtg ccggttcgag
    16321 tccggccttc ggcaccatta gcacttccaa gatcatccga gaaagtccaa ttgttcctta
    16381 taattcaatg agtacagtga ttcttatgtc cggagtcgtc cgagattgtc cgttgaaatc
    16441 cggatgccat tgggggcata attaggggca tcttgacttc gattagaaat gtgcccccaa
    16501 atgaagctca acgccagact ggtcgagacc gcaaagccaa aagacaaaac ctacaaaatg
    16561 gccgatggtg gcggtttgta tcttgaggtt tcggccaagg gatctaaata ctggcgtatg
    16621 aaatacagac gcccttcaga caaaaaagag gatcgcctcg cttttggcgt ttggcctact
    16681 gtgacacttg cccaggcaag ggcaaaacgt gatgaagcta aaagattatt agtgcagggt
    16741 attgatccaa aagccgagca gaaagcggct caggccgaga aatcgggggc atatactttc
    16801 gaaacgatcg ctcgtgaatg gcatgccagt aacaagcgct ggagtgaaga ccatagagcg
    16861 cgcgttcttc gctatcttga gctttatatc ttccctcata tcggatcgtc cgatatccgc
    16921 cagcttaaaa caagccacct gttagccccg attaaaaaag ttgatgccag tggtaagcat
    16981 gacgtcgcgc agcgtcttca gcagcgtgtt acagctatta tgcgttatgc cgtacagaac
    17041 gattatatcg actcaaaccc agccagtgat atggctggtg ctttatcgac agttaaaaca
    17101 cgacactatc cagcgttacc ctcccatcgt tttcccgaat tattggcacg tctcgctgca
    17161 tatcgtggtc gtataatgac aaaaattgcg gttcagcttt ctttactaac atttgttcgc
    17221 tcaagtgaat tacgctttgc tcgttggaga gagtttgatt ttgataacgc aatttggcgt
    17281 attccggcaa aacgtgaaga aattaaagga gtgcgttact cataccgtgg tatgaaaatg
    17341 aaagaagaac atattgtgcc attaagtcat caagccatgg aactcttgag gcagctaaca
    17401 caaattagtg gtgataagga gtttcttttt cctggggatc acgatgctac gaaagttatg
    17461 agtgaaaata cggtaaacaa tgctttacgc tcaatgggat atgacaccaa aactgaagtt
    17521 tgtgggcatg gatttagaac aatggcgcgt ggagccttgg gagagtcagg attatggagt
    17581 gatgacgcaa tagagcgtca gttgagtcat gctgagcgta acaatgtgcg tgctgcttat
    17641 attcatactt cagagcattt agatgaacgt cggctaatga ttcagtggtg ggctgactac
    17701 ttgggagcaa ataaagaaaa atatatcaca ccttatgatt atgctaagtc tgaatagcgg
    17761 gtaacatcta ttgttatatt ttatgttaac actatcacgt tttctgagat atcaatctaa
//
LOCUS       DBANUU010000088        17708 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000088 DBANUU010000000
VERSION     DBANUU010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.088"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            11..202
                     /locus_tag="ACIE68_003087"
     CDS             11..202
                     /locus_tag="ACIE68_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909362.1"
                     /translation="MLGKVFFVVLSCSLLLNPLATYARNYPCSGKKGGVSHCTSDGKF
                     VCNDGTISKSKKICTKNSR"
     gene            391..1410
                     /locus_tag="ACIE68_003088"
     CDS             391..1410
                     /locus_tag="ACIE68_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFX5909363.1"
                     /translation="MAVRKLTTGKWLCECYPAGRSGRRVRKQFATKGEALAFERHTME
                     ETEAKPWLGESVDRRTLKDVVELWFKLHGKSLTAGQHVYDKLLLMVDALGNPLATDLT
                     SKMFAHYRDKRLTGEIYFSEKWKKGASPVTINLEQSYLSSVFSELSRLGEWSYPNPLE
                     NMRKFTIAEKEMAWLTHEQITELLYDCNRQSPLLALVVKICLSTGARWREAVNLTRSQ
                     VTKYRITFVRTKGKKNRSIPISKELYEEIIALDGFNFFTDCYFQFLSVMDKTSIVLPR
                     GQLTHVLRHTFAAHFMMSGGNILALQKILGHHDIKMTMRYAHLAPDHLETALRFNPLA
                     TMVTT"
     gene            1576..2043
                     /locus_tag="ACIE68_003089"
     CDS             1576..2043
                     /locus_tag="ACIE68_003089"
                     /inference="COORDINATES: protein motif:HMM:NF024671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HFX5909364.1"
                     /translation="MININQLCDYIITKILASGETMSNLKLQKLAYYADAWYLAFFDK
                     KLVDEEFQAWIHGPVSRAIYNRFSSTKSLYSDITIADRTPGFDLSSIPAATAAHIDGV
                     LEVYGAFSGAQLEDMTHKEEPWIKAREGYRPSARCEVVIDRNITRDFYRSRLN"
     gene            2049..2804
                     /locus_tag="ACIE68_003090"
     CDS             2049..2804
                     /locus_tag="ACIE68_003090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909365.1"
                     /translation="MVNAYRPSLGANQPTAAQTPKTPSAPEKKWAFSFRYWRQIDNFG
                     LDRTNSGWFVSLIGKLSELSQKDVKGFITSGADREAWRYHSINWEQRNIPVQRSQLTW
                     IDTDYLENDEEYPLLQFQISTALGRVVGFWDENQIFNIVLLDPLHNIQPTRSHNYRVD
                     YCAPLETPYSLLLCRVDDIRRTCRCSDPECSFKRRIEQFEEIPIQTNTLVHYVSDMEW
                     AETQQFIEDGLISDAKEVFSWGLLAIKDQSSPS"
     gene            complement(2949..3485)
                     /gene="rcdA"
                     /locus_tag="ACIE68_003091"
     CDS             complement(2949..3485)
                     /gene="rcdA"
                     /locus_tag="ACIE68_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFX5909366.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            3569..4777
                     /gene="ybjJ"
                     /locus_tag="ACIE68_003092"
     CDS             3569..4777
                     /gene="ybjJ"
                     /locus_tag="ACIE68_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5909367.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHISLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            4777..5592
                     /gene="ybjI"
                     /locus_tag="ACIE68_003093"
     CDS             4777..5592
                     /gene="ybjI"
                     /locus_tag="ACIE68_003093"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFX5909368.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            5678..5962
                     /gene="ybjH"
                     /locus_tag="ACIE68_003094"
     CDS             5678..5962
                     /gene="ybjH"
                     /locus_tag="ACIE68_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFX5909369.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(6003..7235)
                     /gene="mdfA"
                     /locus_tag="ACIE68_003095"
     CDS             complement(6003..7235)
                     /gene="mdfA"
                     /locus_tag="ACIE68_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFX5909370.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            7520..8116
                     /gene="ybjG"
                     /locus_tag="ACIE68_003096"
     CDS             7520..8116
                     /gene="ybjG"
                     /locus_tag="ACIE68_003096"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFX5909371.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGFTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            8174..8932
                     /gene="deoR"
                     /locus_tag="ACIE68_003097"
     CDS             8174..8932
                     /gene="deoR"
                     /locus_tag="ACIE68_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFX5909372.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(8979..10181)
                     /gene="dacC"
                     /locus_tag="ACIE68_003098"
     CDS             complement(8979..10181)
                     /gene="dacC"
                     /locus_tag="ACIE68_003098"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFX5909373.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQMKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            10428..11054
                     /gene="gstB"
                     /locus_tag="ACIE68_003099"
     CDS             10428..11054
                     /gene="gstB"
                     /locus_tag="ACIE68_003099"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFX5909374.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(11051..12166)
                     /gene="yliI"
                     /locus_tag="ACIE68_003100"
     CDS             complement(11051..12166)
                     /gene="yliI"
                     /locus_tag="ACIE68_003100"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFX5909375.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(12277..12660)
                     /gene="bssR"
                     /locus_tag="ACIE68_003101"
     CDS             complement(12277..12660)
                     /gene="bssR"
                     /locus_tag="ACIE68_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFX5909376.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            12873..14198
                     /gene="rimO"
                     /locus_tag="ACIE68_003102"
     CDS             12873..14198
                     /gene="rimO"
                     /locus_tag="ACIE68_003102"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFX5909377.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(14245..15573)
                     /gene="dgcI"
                     /locus_tag="ACIE68_003103"
     CDS             complement(14245..15573)
                     /gene="dgcI"
                     /locus_tag="ACIE68_003103"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFX5909378.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(15581..>17708)
                     /gene="pdeI"
                     /locus_tag="ACIE68_003104"
     CDS             complement(15581..>17708)
                     /gene="pdeI"
                     /locus_tag="ACIE68_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFX5909379.1"
                     /translation="LKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKI
                     VVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCIS
                     FTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLARE
                     KLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFA
                     FIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIK
                     NSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVE
                     AVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDF
                     DSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEI
                     EENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFIT
                     IAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTT
                     IMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFY
                     EINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGC
                     VQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         4633 a         4375 c         4074 g         4626 t
ORIGIN      
        1 ggaggaaatt atgcttggta aggtattttt tgtggttttg tcatgttctt tgttattaaa
       61 cccactagct acctatgcta gaaattatcc ctgctcaggg aaaaagggag gtgtttctca
      121 ctgtacctca gatggcaaat tcgtttgcaa tgatggaact attagtaaat ccaaaaaaat
      181 ctgtactaaa aactcacgat aacttttgct tttatatctg cgcctaaaat aaaaatgagc
      241 cacaggttaa ccgcaaaagt tacatgctca catagcaaaa agaatagcct acttcattat
      301 ggcttcagtg agatgtatgg tcgcaggatt tcatatattg aaactggtta tacatacagt
      361 aaaaatgctc tctattggag ggcatttttt atggctgtac gaaaactcac cacaggaaaa
      421 tggctttgcg aatgttaccc cgccggacgt agtgggcgtc gtgtgcgtaa acaattcgcc
      481 accaaaggcg aagcactggc ttttgagcgt cacacgatgg aagaaaccga agcaaagccc
      541 tggctaggtg aatcagtgga tcgtcgaaca ctaaaagacg tggttgagct atggttcaaa
      601 ctacatggta aatcactgac tgctgggcag catgtctatg acaaattgct gctgatggtt
      661 gacgctctgg gcaatcccct tgcaactgat ctcacctcta aaatgtttgc ccactatcga
      721 gataaacgcc tgacaggcga gatctacttc agcgagaaat ggaagaaagg agcaagcccg
      781 gtcaccatta acctggagca aagctatcta agtagtgttt ttagcgaact atcccgcctg
      841 ggcgaatggt cgtatccgaa cccactggag aacatgcgaa aattcaccat cgcagaaaag
      901 gagatggcct ggctgacgca tgaacaaatt accgagctat tgtacgactg caaccgccaa
      961 agtcccctgc tcgctcttgt cgttaaaatc tgtctgagca ccggagcacg ctggcgcgaa
     1021 gctgtgaacc taacccgttc tcaggttacg aaataccgga tcacattcgt caggaccaaa
     1081 ggcaaaaaga accgaagcat tccgattagc aaagagctgt atgaggaaat cattgcccta
     1141 gacggtttca atttcttcac tgactgctac ttccagtttt tatctgtaat ggataagact
     1201 tctattgtgc ttccacgcgg tcagctaaca cacgttctgc gtcatacgtt cgcagcacac
     1261 tttatgatgt ccggcggaaa catcctggct ctgcagaaaa tccttgggca tcacgacata
     1321 aaaatgacca tgcgctatgc tcatctggct cctgaccacc tagaaacagc gctacgtttt
     1381 aatccattag caactatggt aactacgtaa aatgaaagca ctgctcatcc atattcatca
     1441 cattttagca cgtttgaatc tcgatttatt cagctatgta tgccactata agttaacttg
     1501 gtattcaaag atttctaacg gtcgatatct ttaataagat tggctgctta gacaccaagc
     1561 ctagagggat aaataatgat aaatattaac caattatgtg attacatcat cacaaaaatc
     1621 ttagcttcag gtgaaactat gtctaatttg aagctgcaaa aattagctta ttacgcagat
     1681 gcttggtatc tagctttttt cgataaaaag ttagtggatg aggagtttca agcttggatt
     1741 catggcccag taagccgtgc gatttataac cggttctctt ctacaaaatc actgtattcg
     1801 gacataacga ttgcagatcg cactcctggg tttgatcttt catccattcc agccgcaacc
     1861 gcagctcata ttgatggcgt tcttgaagtc tacggtgctt tttctggcgc acaacttgaa
     1921 gatatgactc ataaagaaga accatggatt aaggctcgtg agggttacag accttcagca
     1981 cgttgtgaag tagtcatcga tcgcaacata acaagagact tttatcgtag ccgattaaat
     2041 taatagccat ggtgaacgcc taccgccctt cattaggggc aaatcaacca acagcggcac
     2101 agaccccaaa aacgccctct gcacccgaaa aaaagtgggc attttctttt cgttattggc
     2161 gtcaaattga taattttggg ttagaccgta ccaattcagg ctggtttgtt tcactaattg
     2221 gaaaattatc tgagctttct caaaaagatg tcaaaggctt tattaccagt ggggccgata
     2281 gagaggcatg gcgctatcat tcaataaact gggaacaaag aaatataccc gtacaacgta
     2341 gccagttgac atggattgac actgattacc ttgaaaatga cgaagaatac cccttactac
     2401 aatttcagat atctactgcc cttggtagag ttgttggctt ttgggatgag aaccaaattt
     2461 ttaatattgt gcttttagat ccccttcata acatccagcc aacacgatct cataactatc
     2521 gggttgacta ctgtgcacca cttgagaccc cctactccct tttactctgt agggtagatg
     2581 atataagacg cacatgccgt tgttcagatc ctgaatgtag tttcaaacgt cgaatcgagc
     2641 aatttgaaga aatcccaatc cagacaaata cgctagtaca ctatgtcagt gatatggaat
     2701 gggctgagac acagcaattt atagaggatg gtttaataag cgatgccaaa gaagttttct
     2761 catggggact tctagctatc aaagaccagt catcaccttc ttaatggcga caaagtggcg
     2821 gcagcggttg gcattacccc gtaataacca ccactgacca ccaacccaac ttattgtttt
     2881 tactctaact tattgttttc attaacccgt ttacataaat gggttttttg ttatctaaaa
     2941 tttatctact accctgcaac cctctcaacc atcctcaaaa tctcctcgcg cgatagcggc
     3001 ttacggtcgg tgacaaaatg cagcgtcatc ccctcaataa acgcatcaag cgcgcgggcg
     3061 gttccgggtt caaaccattg ttcgagcgtt tgctgactgc gctgcatcca gttttgcatt
     3121 accgttttta atagcggttt tcggctagcc agcgcgtaga gctggtacat cagctccatg
     3181 ttatccggcg ttgcaacctg tgagctgtag atcatatcgg tgatagcctg gcatgcgcct
     3241 tgagcatcac taacatcgct aaaaaatgcc tgatattgcc gggacataat ctcagtaaaa
     3301 ctgctgaacg cctccagcaa caactcatca attcctgaaa aatagtaggt catcgatccc
     3361 aacggtaccc cggcaagggt agcaattttg cggtgcgtaa cagcatgtat tccgtaaagt
     3421 ttcaccgcct ccagcgtggc ctggataatt ttttctcgcc gttgcggatc gttagcgcga
     3481 cgcatgtcat ttcctctgtc tgcaattgtg tacaaatgta cacaaccttg ctaatgttgt
     3541 cttccctctt cttattttga cgtgatctat gaccgtaaat tcttcacgta atgcattgaa
     3601 acgccgaact tgggcgctgt ttatgttctt ctttttgcca ggcctgttaa tggcgtcctg
     3661 ggcaacccgt acgcctgcta tccgcgatat tctctctgtc tcgatcgctg aaatgggcgg
     3721 tgttctcttt ggtctgtcga tcggttcgat gagcggtatt ctctgctcgg cgtggttagt
     3781 gaaacgcttt gggacacgta atgtcatcct ggtcacgatg tcctgcgcat tgatcgggat
     3841 gatgatatta agtctggcac tctggctgac atcgcccctg ctctttgccg ttggtctcgg
     3901 cgtctttggg gcaagttttg gttctgcgga agtggcgata aacgttgaag gtgccgccgt
     3961 tgagcgagaa atgaataaaa cggttttgcc gatgatgcac ggtttttata gcctgggcac
     4021 gctggcaggc gctggtgtcg ggatggcact gacggccttt ggcgttccgg caacggtgca
     4081 catttcattg gcggcgctgg taggtatcgc gcctatttat atcgccattc aggcaatccc
     4141 tgacggtacg ggcaaaaatg ctgccgatgg cacccagcat ggcgaaaaag gcattccttt
     4201 ttatcgcgat atccagttgc tgttgattgg tgttgtagtg ctggcgatgg cctttgccga
     4261 aggttctgcc aacgactggt tacccttatt aatggttgac ggtcacggtt ttagccctac
     4321 ttccggctcg ctgatttatg ccggttttac cctggggatg accgttggac gcttcaccgg
     4381 tggttggttc atcgaccgtt acagtcgcgt tgccgtggtt cgggccagtg cactaatggg
     4441 ggcgttgggt attgggctga ttatttttgt cgatagcgcc tgggtcgcag gagtgtctgt
     4501 tgtactttgg ggactgggtg cctcgctggg cttcccgctg accatttctg ccgccagcga
     4561 taccggcccc gatgcaccaa cccgcgtcag tgtggtagca acgaccggtt atctggcttt
     4621 cctcgtcggg ccgccgctgc tgggctatct cggcgaacat tatggattac gtagtgcaat
     4681 gctggttgta ctggcgctgg ttattctcgc ggctattgtc gcgaaagccg tcgccaaacc
     4741 cgataccaaa acgcagacgg cgatggagaa tagttgatga gcattaaatt aattgcggta
     4801 gacatggatg gtactttctt aagcgatcaa aaaacctata accgtgagcg gtttatggct
     4861 cagtatcagc aaatgaaagc acaaggcatt cgctttgtgg tcgccagcgg gaatcaatat
     4921 tatcagttga tctctttctt ccctgaaatt gcgaatgaaa ttgcctttgt ggctgaaaac
     4981 ggcggctggg tagtgagtga aggcaaagat gtttttaatg gcgagctgtc gaaggatgcc
     5041 tttactactg tcgtggaaca tttgctgacg cgcccggaag tggaaattat tgcctgcgga
     5101 aaaaatagcg cctatacgct caaaaaatat gacgatgcca tgaaaacggt ggcggaaatg
     5161 tattatcacc gtctggaata cgtcgataac tttgacaact tagaagatat tttctttaag
     5221 tttggcctga atctttccga tgaactgatt ccacaagtac aaaaagcatt acatgaggcc
     5281 atcggcgata ttatggtgcc agtccacacc ggcaacggca gcatcgatct gattatcccc
     5341 ggcgtacata aagccaatgg ccttcgccaa ctgcagaaat tatggggaat agacgacagc
     5401 gaagtggtgg tctttggcga tggcggtaac gatattgaga tgctgcgtca ggcaggcttt
     5461 agttttgcaa tggaaaatgc cggcagcgcg gtcgtcgcag cggcaaaata ccgggcaggc
     5521 tccaataacc gtgaaggcgt actggatgtg atcgataaag tgcttaaaca cgaagcgcca
     5581 tttgaccaat aaaaacggca gcacgcccgg caacacaata cctgaatgaa ggctgcataa
     5641 ttcgcggaaa ttaacccgct gcattatttc aggcgaaatg attatgaaaa attgtctact
     5701 gttgggcgcg cttttaatgg gctttactgg tgtggcgatg gcgcaaagtg tcaccgtcga
     5761 tgtgccgagt ggatacaaag tggttgtagt tcctgattca gtcagcgttc cacaagcggt
     5821 cagcgtcgcg actgtgccgc aaaccgtcta tgtggctcct gcgccagctc cggcttaccg
     5881 cccgcatcct tatgctcgcc atcttgccag cgtaggtgaa gggatggtta tcgaacacca
     5941 gattgacgac catcaccatt aattcgagaa tgcctgatcg cacaaagcag tcaggcattt
     6001 ttttaccctt cgtgagaatt tcccatctgt ttatctttta aaaagataac catcagcgac
     6061 agccacaaaa ttccgttgac aaggttgaag agattaaact gtccgttgcc cccgttcagc
     6121 caggcatgtt tgctgatttc aataccaacg gtaaagatca gcatttgcag cattcccatc
     6181 gccgcagaaa ccgtaccttt actcatatcg ctggcaaaca gggttaatcg caccagtccc
     6241 gcattcgcca gaccaatacc gaaagcataa atacttaacc cggcagtcat ccataaatac
     6301 gcgtgcgatg agataaccgt tgccgcagca gcgaccaaca gaccaatcat aatcggccag
     6361 ccgcccataa ttatcagcga acgtacggtg cggcgcgagg tcaaacgcgc taacaacaag
     6421 ttacccgcaa ttaacgcccc gaaaataggc acttgcagca agccatattc atagctgctc
     6481 aactgctcgc cggtaatgat gataatcggc gactgggcga tccacgccag caatggcagg
     6541 ctaacgaatc ccagcgccag cgcccccgcc acaaagcggc cgttcttcag caccagctta
     6601 tagtcacgac cgagttcttt cagtgacagt ttctcgccta tacgcgtggc ggtttcaggc
     6661 atagctcgtt gcagaccgaa aaaggagatc gctgccaatg cggcaaacaa aacaaacatc
     6721 ccctcccagg gcagcacatg gatccacgcc gcgcccacca gcggaccaag tagcggagca
     6781 atcagcgcca cgttcgccat cagcgcggtg atcttgatac aaaccgcctc ttcgaaggat
     6841 tcctgaattg cggcgtatcc cacagcgcca atgaaacaga ggcttatgcc ctgcaagaag
     6901 cgcaacaggg tgaattgttc aatgttttgc gccagcaata ttgccagaca ggtgatgata
     6961 aaccacacca cccccgccag catcaccgga cggcgaccaa tacgatccga cagcggcccc
     7021 aggagccatt gtaaaaacat cccgcccgcc agatatgcgg tcattgaagt aggaacccaa
     7081 tcaatgcccg cctgatattg ttccaccacg gccaacatac cgggttgaat catatcgttg
     7141 ccgatatagg ttgaaaattc gtaaagcacc agacagagag ggaaaagtaa cgcctgacgt
     7201 ccaagcctgg caccggaagc taatttattt tgcatgcaat ttcttcgcca ataataatcg
     7261 cgcagagttt aataaaagca cagctaacga gaaagcgaat tttgtagctg aaaccacggt
     7321 taagcacatt cttacattat tgcgagtata gctacgcttt ctttaagttt tatttaacct
     7381 atgcccgtta caattaccca ccgtaaacag gccgcttgag ggaaataaga cgatgccgct
     7441 ttacccagtt taacctgcac tttattctca acgacttgcc tgtattggct cccttttaat
     7501 cactttgcgt cgggaagtta tgctggaaaa tttgaatctc tctctattct ctcttattaa
     7561 cgcgacgcca gactcggctc cgtggatgat ctcgttggcg atttttattg ctaaagattt
     7621 gattaccgtg gtaccgctgc tggccgcggt gctttggttg tgggggttta cagcacaacg
     7681 gcaactggtg ataaaaatcg ctatcgcgct ggcggtcagc ctgtttgtgt cctggacgat
     7741 gggacatctt tttccgcacg accgaccctt tgtcgaaaat atcggctata acttcctgca
     7801 tcatgcggcg gatgactcat tcccaagcga tcacggtacg gtgattttca cctttgcact
     7861 ggcattttta tgctggcatc gcctgtggtc cggctcactt ttaatggtgc tggccgtcgt
     7921 cattgcctgg tcgcgcgttt atcttggcgt ccactggccg ctggatatgc tcggtggatt
     7981 gctggcaggt atgattggct gccttagtgc ccagattatc tggcaagcga tggggcataa
     8041 actctatcaa cgtctgcaat cgtggtatcg cttctgtttt gcattaccga tccgcaaagg
     8101 ctgggtgcgt gactgatctc tgctaaaaag tgtagtattg agcggctcgc ttcaataact
     8161 attcagaggg attatggaaa cacgtcgcga agagcgtatc gggcagctgc tgcaagaatt
     8221 aaaacgcagc gataagttac atcttaaaga cgccgccgcc ctgcttgggg tttcggagat
     8281 gacgattcgt cgcgatctga acaaccacag tgcgcccgtc gttttgctcg gcggctatat
     8341 tgttctggaa ccgcgcagtg ccagccatta cctgttaagc gatcaaaaat cccgcctggt
     8401 ggaagaaaaa cgccgggcgg caaaactggc tgcgacactg gtagaacccg atcagaccct
     8461 cttttttgac tgtggcacca ccacgccgtg gattattgaa gcgattgata atgaaatccc
     8521 ttttaccgcc gtttgttatt cgctaaatac ctttctggcg ctgaaagaga aaccccattg
     8581 ccgcgcattt ctgtgcggtg gtgaatttca cgccagcaac gccattttca aacccatcga
     8641 ttttcagcaa acgctgaata atttttgccc ggatatcgct ttttattctg cggcgggcgt
     8701 gcatgtcagt aaaggcgcta cctgttttaa tcttgaagag ttgccggtaa aacactgggc
     8761 catgtcgatg gcgcaaaagc atgtgctggt tgtcgaccac agtaaatttg gcaaggtgcg
     8821 tccggcgcgc atgggtgacc tgaaacgctt tgatattgtg gtgagtgatt gttgcccgga
     8881 agatgagtat gtgaagtacg cgcagacgca gcgcattaag ttgatgtatt aatgacgtat
     8941 aaccggatga cgtttcgcgc catccggtta tcagaagatt aagagaacca gctgccgaac
     9001 cactgatgga atttcatcat cacgaaatcc cacatccgac caaagaatcc gccctcttcc
     9061 acattttcca tcacgatcag cggacgctgc tcaatggatt taccgttaag ctggaaatca
     9121 atggtcccga caacctgacc ttttttcagc ggtgcggtaa gctgcggttc cgttaacgtg
     9181 tagctcgcct tcaggttttt catctgccca cgcggtatgg tcactgagcc cgcttcgcct
     9241 gccccaagat tcacttcgct cttatcacca aaccagacgc gctgagtcac aaaggtggca
     9301 tcaggtttaa ttggcgtcac ggtttcaaag aagcggaaac cccaggtcaa taatttctca
     9361 gactcattaa aacggatacg gtcagttttc gcccccagca ctacggagat taaacgcata
     9421 tcgccctggg tagccgaagc aaccagatta tatcccgcgc ctgccgtggt tcctgtcttc
     9481 atgccatcaa cattcagatt gctgctccac agcagacggt tacggttagg ctgacgaatt
     9541 ttgttgaagg tgaactcttt ctctttatga atggcgtact cttccggcac atcgtggatc
     9601 aatgctttac ccagcaatgc catatcgcgc gcggtactga actgccccgg cgcatccaga
     9661 ccgtgcaccg tctggaaggt agtgttggtc agacccagtt ttttggcata accattcatc
     9721 aaaccaatga atgactcctg gctcccggca acgtaatcgg ccagcgcaat acaggcgtca
     9781 ttaccggact ggataatcac acctttgttc aagtctgcca ccgaaacctg atcgcccggt
     9841 ttgaggaaca ttaccgatga accacgcagt gccggatttc ccgtcgccca ggcatcttta
     9901 ccgaccgtca ccatatcggt gagtttaatc ttatcggcct taagcgcctg cccaaccaca
     9961 tagctggtca tgattttagt caggctcgcg ggatccagtt tctcatccgc gttgccttct
    10021 gccagcactt taccgctggc gtaatccatt aaaatccatg cacgcgcatc cacgctcggc
    10081 gcttcaacgg tttgttccgc cgcgaatgcc gttggggcaa aaaggaataa aaatgcagaa
    10141 cccgctgcaa gaccacgaag gagagaggag tattgcgtca taaatgccac ccgagtatcc
    10201 attcaaaaaa aatacgttat atcgccgtat gataagttcg gtgattaata acgcacacac
    10261 aaacttaaag aaaccgtaag ttggtaaagt ttttaaagtt tatgcaatat cctgccgctt
    10321 cgctgcttct ggtgtcattt ttttgccagc tgttgcttaa aagttaactt tacttcacca
    10381 tcggagtcct gttcagcctg tggctgcctg tcggaaaagg ggtgagtatg attacgctgt
    10441 ggggtcggaa taattcaacc aacgtaaaaa aagtattgct gacgctcgaa gaactggaac
    10501 taccttatga gcaaattctc gcgggccgtg agtttgggat aaatcacgat gctgattttc
    10561 tggcgatgaa ccctaacggg ctggtgccgt tgttgcgtga cgacgaaagt gatctcattc
    10621 tttgggaatc aaacgccatt gtccgctatc tggcggcaca gtacgggcaa aaacgtctgt
    10681 ggatcgactc accggcacgt cgtgcggaag cggaaaaatg gatggactgg gcaaaccaga
    10741 cgctcagtaa tgctcatcgc gggatcctga tgggattagt cagaacacca ccggaagagc
    10801 gcgatcaggc cgccattgat gccagttgca aagagtgcga cgccctgttt gccctgctcg
    10861 atgcggaact ggcaaaagta aaatggttct ctggcgacga gtttggtgtg ggcgatatcg
    10921 ctatcgcacc gttcatctac aatttgttta acgttggcct gacctggaca ccgcgtccaa
    10981 atctgcaacg ctggtatcag caactcactg aacgccccgc agtgcgcaaa gtggtgatga
    11041 ttcccgttag ctaattgcgt gggctaactt taagtaattc cccactggac tcgtcggtga
    11101 gaacgtataa ataaccgtcg ggtccagtgc gaacatcacg aattcgctgc cctctgtccg
    11161 ttaaaatacg gccatcttct gtcactttgt cgccgttgac gctcatcaca atgacatctt
    11221 tatctttcag cgcgccaata aataattttt gctgccactg ggggaattta tcgctgttat
    11281 agaaggccat gccgctcaca gcgggcgaat ctttccagta aaaaacaggt tgctcggtcc
    11341 cggcgacgat ctcccctttc gcttccggta tcttaaagcc tgaatagttg attccccagg
    11401 ttgccagcgg ccagccgtag tttttgcctt tttgcgggat attaatttca tcaccaccgc
    11461 gcgggccatg ttcattcagc cacagtgcat tactccacgg attcatcgcc attccttgcg
    11521 gattacgaat gccataagac cagatctcgg cgcgcacacc ggattccttt ataaaaggat
    11581 tatcatccgg gatttcgccc tggtcggtca gacgcaccag tttgccctgt aatttatcca
    11641 gatcctgcgc cgtcgggcgc tgattgtttt cgcccagagc aataaaaaga taacctttac
    11701 cgtcgaatac cagccgcccg ccaaaatggt tgccggtaga cagttttggc atctggcgga
    11761 agacggtgcg gaagtcggtc acttttgaga gatcatcact taagcggcca taacccacag
    11821 cagttccggc tttgccatca tcgccaactt cggaataact taaccagatg cggcgagact
    11881 gagcaaaatc aggcgctaaa accacgtcca gcaggccgcc ctgcccgtgc gcccaaacgt
    11941 ccggaactcc ggaaagcggc gcagataatc cttttcctgc ttgccagtga cgcaactcgc
    12001 cgccgcgcag agtgattaac ataccgtgat tatcgggtaa aaaggccagt gcccagggat
    12061 ggtcgagttt gtcttgcagt acttcgacat ttaccgttgc aggagccgcc cagagagcgg
    12121 aagaaagaca aataaggggc acaaggaaaa aggattgtcg atgcataacg cctccggatt
    12181 gactctggct taaagcgtag tcagtggagg agataacaaa ttcattttta caaaaactta
    12241 aacatgaagg gggagacgct ttctccccct tagttttcag gccttctcaa gcatggcgtg
    12301 cttctgcagg ctctggatac tcagcgttaa gctcatcaga caattttcaa gcttatcggc
    12361 gttgacggta ataacagtcg ggcaatcatg gtgcccactc atcaaacata ctgcggctgt
    12421 cgctaatgct tcttcagcat gatgaagagc actccactct tcctgatcca gatgaagatt
    12481 caaccgcagc gatttatcgt gcagttcgcg attcagttta aaaaagttat ctcgtagatg
    12541 attgctttcg ctgacggaca tgtatccttt tgccttcctc agttgcacgt atgcagcgag
    12601 gtcaacgcgt gcgtcgatca accggttcag cagatcgatt cgctgtctgt caacgaacat
    12661 acttcgatcc tcctctttcc agccttgttg caattaaata gtatggctat tttttgagca
    12721 aaatgctgtc caggatcaat tcttcacgcc gaattaacaa aatttacatt tccttcgcct
    12781 ccgccagcga atgagattgc gatacaaatt ttatccagaa ctctgtagaa tccctgccct
    12841 gacctttcct gtaactgaat attttttaag ctatgagcaa agtaactccc cagccgaaaa
    12901 tcggctttgt ttcccttggc tgtccgaaaa accttgtcga ttcagagcgt attctcaccg
    12961 aactccgcac tgaaggttat gacgtggtac cgagctatga cgatgcggac atggtgatcg
    13021 tcaacacctg cggctttatt gacagcgcgg tacaagaatc actggaagcc attggtgaag
    13081 cgttgaatga aaacggcaag gtaattgtga ccggttgtct gggggcaaaa gaagatcaga
    13141 tccgcgaagt ccacccgaaa gtgctggaaa ttaccgggcc tcatagctat gagcaggttc
    13201 tggagcacgt tcatcactac gtgccaaaac cgaaacacaa cccattcctg agcctggtgc
    13261 cagaacaagg tgtgaaactg acgccgcgtc attatgccta tctgaaaatt tcggaaggct
    13321 gtaatcaccg ctgcaccttc tgcattattc cgtctatgcg cggcgacctg gtgagccgtc
    13381 cgattggcga agtgttaagt gaagcgaaac gtctggtaga tgcgggcgtt aaagagattc
    13441 tggtgatctc gcaggatact tccgcctatg gcgttgatgt taaacatcgt actggcttcc
    13501 acaacggcga gccggtaaaa accagcatgg tcagcctgtg cgaacagcta tcgaaactgg
    13561 ggatctggac acgtctgcac tacgtttacc cttatccgca tgtggacgac gtcatcccac
    13621 tgatggcaga aggcaaaatc ctgccgtatc tggacattcc gttgcagcac gccagcccgc
    13681 gcattctcaa actgatgaag cgtccgggtt ctgtagatcg ccaactggcg cgcatcaaac
    13741 agtggcgcga aatctgcccg gaactgaccc tacgctcaac ctttattgtc ggcttccctg
    13801 gcgagacaga agaagatttc cagatgctac tcgacttcct gaaagaagcg cgtctggatc
    13861 gcgttggctg ctttaaatac agcccggttg aaggtgcaga cgccaatgcc ctgcctgacc
    13921 aggttccgga agaagttaaa gaagaacgct ggaaccgttt catgcagctg cagcagcaga
    13981 tttccgccga gcgcctgcaa gagaaagtgg gccgtgaaat tctggtgatt atcgacgaag
    14041 tggacgaaga aggcgcgatt ggtcgcagca tggcagatgc accggaaatc gacggcgcgg
    14101 tttatctcaa tggtgaaacc aacgttaagc cgggtgatat cctgcgtgtg aaagtcgagc
    14161 acgccgatga gtacgatttg tggggtagcc gggtttaatt aattcaggcc gggcaaaatc
    14221 atttgtccgg cctgcgaata atgattactg ccagctaatc tgagccctgc cgtttttctt
    14281 actcttatac agccgctcat ccgccagcat tttcaagcgt tcgacggtgg tacattcatt
    14341 tgaatgcgct acaccgatac tggtacttaa attaatgctt cccacctgga aatgaacgat
    14401 ttcttttaga atcgatagca actcttccag cgttccaccc gtataaatca ccgcaaattc
    14461 gtcaccgccg aagcggtaga ttttaccttt atctaccggg atctgtgatt taaggatttt
    14521 caccactttc atcaacactt catcgcccag caaatgaccg aaggtgtcgt taatgttttt
    14581 gaatttatcg acgtcaaaca ggcacaaata accgcctttt tccggcagcg cttttaaatc
    14641 ttcgtcaaag gattttctgc ccattgcttt ggtcaggtcg tccaggaacg ccgagttata
    14701 catactgtag agttttttga catcggcagc cgaaaagaaa agcccgccat ccagtacgcg
    14761 ttcaaaatgg ttattaatga tggcttcttt ctccacaata ccacgattaa tcactctgcg
    14821 taccagatag aggcaactgg tacccgatat cactgccacc aatgcggcaa agaaaataat
    14881 caatgtacag acccgcaaat aaacatcttt tttatacgca aatacgtaca atccggtcgg
    14941 caaataggcg aagtgctcca tcgcttccga aacggtgaag atggcgtttt tagcattata
    15001 ttcatcaccg gtcaccggtt taccgttctt aaatagagag aacatcaggt tctcgctgtc
    15061 catcgccagc gattttgaca gtaaagaaaa acgctccaga ccaagcaaaa atatcagact
    15121 acgattgtga gttaccggat agaggtaata gacgaaggtt ttaccgtcaa aagagataaa
    15181 ccctgtattt ttgacatgtc gatacttcac aacctgcata atatctgcac aataattttt
    15241 ctcgaactcc tttacctgaa tacattcgtc actggaaatc agataaggac cgacatcgta
    15301 caaataagag ggggaaaccg agtcggcgta agctgtgcgc aaaccaacca acagctgttc
    15361 ctccgtggga tttagcgtgt tgataagatc aattgccacg ctacgccgat attcagcaat
    15421 atttgccacc gcaaaagcag tatcaataac ggactgtttc aggctataca cccgttcctt
    15481 taccatttga gtgtagatcc cgcaggcaac tgctgaaatc atgataaaaa tcagcagact
    15541 gactgtaagt acgaacttat tgattctgga catacgtaaa ctactctttt actaattttc
    15601 cacttttatc ccaggcggag aatggcatgg gtttctggta cagatacccc tgagcctgaa
    15661 cacatcctaa tttaattaat tcgcgcgcaa cgtcttcgct ctctacgcct tccgcaatca
    15721 ccgggatccg gttatagcgt gccagatcga aaataatgcg cacaattttt cccgcagtac
    15781 cgaaaatatc gccggtaaat tgcgagtcca cctttatgct gtctggatta atttcataaa
    15841 aacgtttcag gtttgacaaa ccggtgccaa agtcatcaat cgatatccgt accccttgtt
    15901 tacgcagttg ttcaatattg agataaaaat gttcattaat tcgctcgatc acggtttcag
    15961 tgatctcaac acagaggctg ttcgccttca ggccattttc tttcatgatg gtggtaaaac
    16021 gctcatgaaa gtgcggttcg tttaactgcc agggcgaaac attaatatgc agtaagaaat
    16081 catccttcag cgacgcacgc tgactaaaat gtctgaattc acccattgcc gttttaataa
    16141 tctgataacc cagctcattg ataaacccga tatcttcagc aatggtaatg aacttcagcg
    16201 gtgaaatgat cccccgctcc gcagaaaccc aacggcacaa cgcctccgcg cccagaatgg
    16261 cgcgggtgtt aatatcaacg atcggttgat agacgaggaa tagttcgcca atttccattg
    16321 catggcgaat attacgcgca atccagatat tctcttctat ttcgttgagt ttcgcgtcat
    16381 cacaaaaaat aaactcggag aacatatggt cacggatact ggaaatcgcc agtttcagtt
    16441 tttctgcgta actccaggcg ttttccccct gcaaaggttc acaaatggca gacttatgga
    16501 tgcgcagatt gcggtagcca aagctgctgt caaaatcgtt taccatcgct attttacggg
    16561 taaatgtctg tacattttcc cgacacagaa gtaaatagag atcggcattg aatgtcacga
    16621 tatcgcagca ttgcgcatat ttctcggcaa ttcgctgaca caatttcctg acgattttat
    16681 tgcgttcatc aacaccaaac accgcttcca cggcttccag accacacagt ttgatgatcc
    16741 ccacagccag atactgatta ttgttggact caataatcgc catattttta ataaaaccgc
    16801 tttcctgatc gtaagagatc agtgagagca atttatttga ccgttcaatt tcggcatctt
    16861 tcatttccgc gatcgaattt ttgatgctga taatctcttt cgcatcgcct tcgcgaattt
    16921 tcagagcctc gatttgatcc gttgagtcgg gcaaaaacgc cagcgtatta accagctcgg
    16981 tcatttcaga aaattgccga cggaacacac gagtggtcat gaaatatacc accagcaaca
    17041 cgataataat gaaagcaaac ggtagataaa aaagtttatc tgatgcgttc aataaattac
    17101 cgccaggata ataaaaatag atttcgaaca atgcattatt tggcactgct gatgtctttg
    17161 caatatactc cgtatcgaca gaatcaataa tattataatt accttcgcta ttataaataa
    17221 tcagtttttc acgagcgagt tgattcttgg caatgatctc atgaccttgt ttcaaaaata
    17281 gaacttcatc attagcgctg atttgatcaa gatactcgtt cagataccgc cagtttaaat
    17341 cgacatgaat tttatctacc gctaacaaat cgtgatcttt gccgttgata cgtagcgtaa
    17401 aagagataca ttttttaccc ggacgatcaa agcaattgta tgccggattc cagctgttgt
    17461 tagagtcatc tgaagccacc gaccacggtc gtttcgatgg gttaaaatcg gcaaaaataa
    17521 cccgccctga ctcgtcgaca agcggtccat ttacttgttc ctgatgataa acaacgattt
    17581 tatcattcgc cctccgcgaa aagagataat atttattatc atcaagtacc agtcccaccg
    17641 agtctaaaaa tggcatcatt tccattttca gaatcacggc gcgttgaatc tcctctgtat
    17701 tttttaat
//
LOCUS       DBANUU010000089        17678 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000089 DBANUU010000000
VERSION     DBANUU010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.089"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            76..579
                     /locus_tag="ACIE68_003105"
     CDS             76..579
                     /locus_tag="ACIE68_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFX5909380.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(646..2103)
                     /gene="nuoN"
                     /locus_tag="ACIE68_003106"
     CDS             complement(646..2103)
                     /gene="nuoN"
                     /locus_tag="ACIE68_003106"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFX5909381.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYVPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2110..3639)
                     /gene="nuoM"
                     /locus_tag="ACIE68_003107"
     CDS             complement(2110..3639)
                     /gene="nuoM"
                     /locus_tag="ACIE68_003107"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFX5909382.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3803..5644)
                     /gene="nuoL"
                     /locus_tag="ACIE68_003108"
     CDS             complement(3803..5644)
                     /gene="nuoL"
                     /locus_tag="ACIE68_003108"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFX5909383.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5641..5943)
                     /gene="nuoK"
                     /locus_tag="ACIE68_003109"
     CDS             complement(5641..5943)
                     /gene="nuoK"
                     /locus_tag="ACIE68_003109"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFX5909384.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5940..6494)
                     /gene="nuoJ"
                     /locus_tag="ACIE68_003110"
     CDS             complement(5940..6494)
                     /gene="nuoJ"
                     /locus_tag="ACIE68_003110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFX5909385.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6506..7048)
                     /gene="nuoI"
                     /locus_tag="ACIE68_003111"
     CDS             complement(6506..7048)
                     /gene="nuoI"
                     /locus_tag="ACIE68_003111"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFX5909386.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7063..8040)
                     /gene="nuoH"
                     /locus_tag="ACIE68_003112"
     CDS             complement(7063..8040)
                     /gene="nuoH"
                     /locus_tag="ACIE68_003112"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFX5909387.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8037..10763)
                     /gene="nuoG"
                     /locus_tag="ACIE68_003113"
     CDS             complement(8037..10763)
                     /gene="nuoG"
                     /locus_tag="ACIE68_003113"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFX5909388.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQEHLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10816..12153)
                     /gene="nuoF"
                     /locus_tag="ACIE68_003114"
     CDS             complement(10816..12153)
                     /gene="nuoF"
                     /locus_tag="ACIE68_003114"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFX5909389.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12150..12650)
                     /gene="nuoE"
                     /locus_tag="ACIE68_003115"
     CDS             complement(12150..12650)
                     /gene="nuoE"
                     /locus_tag="ACIE68_003115"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFX5909390.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12653..14455)
                     /gene="nuoC"
                     /locus_tag="ACIE68_003116"
     CDS             complement(12653..14455)
                     /gene="nuoC"
                     /locus_tag="ACIE68_003116"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFX5909391.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14549..15211)
                     /gene="nuoB"
                     /locus_tag="ACIE68_003117"
     CDS             complement(14549..15211)
                     /gene="nuoB"
                     /locus_tag="ACIE68_003117"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFX5909392.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15227..15670)
                     /gene="nuoA"
                     /locus_tag="ACIE68_003118"
     CDS             complement(15227..15670)
                     /gene="nuoA"
                     /locus_tag="ACIE68_003118"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFX5909393.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16301..17239)
                     /gene="lrhA"
                     /locus_tag="ACIE68_003119"
     CDS             complement(16301..17239)
                     /gene="lrhA"
                     /locus_tag="ACIE68_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFX5909394.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4399 a         4936 c         4591 g         3752 t
ORIGIN      
        1 aaagcgacat gcacatttta ttttccctcg tctgttttaa gatgcgaaac atgaaattca
       61 atgaaggaaa aataaatggg aatgattggt tattttgcag aaattgactc agaaaagatt
      121 aaccaactgc ttgagtctac tgaaaaacct ttgatggata atatacatga cacgctttca
      181 ggattacgcc gactggatat tgataaacgc tgggattttt tacactttgg gctgacaggc
      241 acctctgctt ttgatcccgc caagaatgat ccgctgagtc gtgccgtgct tggtgaacac
      301 agcctcgaag atggcataga tggcttcctg ggattgacct ggaatcagga gttagccgcg
      361 acaatcgacc gactggaatc actggatcga agtgagctgc gtaaacagtt ttccattaag
      421 cgactcaatg aaatggagat ttatccggga gtgacctttt cagaagagtt agaggggcaa
      481 ctctttgcct cgatcatgct tgatatggaa aagctgatta gcgcgtatcg acgtatgctg
      541 cgacaaggga atcatgcgct gacggttatt gttgggtgat ggtgtcagag cgccaaaaaa
      601 aaccgccgag catgtcggcg gctttctgac ttacaaacta acagattaca tcagcggcat
      661 tgctaaccgc acaatgctaa tcagcggttg tggccataca cccagcacca gtaccaacag
      721 tgcagaaatc agcaccacta taccgcccgc gctgtactgc cagtttgatg gtgcatcgcg
      781 acccggttgt tccggggcgt gaagatacag gctcaccgcc acgcgcaggt agtagtagag
      841 gccgattgcc gaaccgacaa ccacggcacc caccagccac cacaagtgtg cctggacacc
      901 gaccgccagc acgtagaact taccgataaa gcccagcgtc atcgggatac cggccagaga
      961 cagcatcatc accgtcatca ctgccgcgag gatcggacga tgccagaaca gaccgcggta
     1021 agagaacagg gaatcagcat ccgggccacg atacgggctg gacatcaggc tgaccacgcc
     1081 gaacgcgccg aggctgctga acagataacc ggccaggtaa acccctaccg cttccatcga
     1141 catctcacca gtttgcagcg caatcagcgc caccagcaga tagccgaggt gagagataga
     1201 tgagtaaccg agcagacgtt tgatgttggt ctggctcagc gccatcaggt taccgaagat
     1261 gatggaggca aaggcgataa tcgccagtac tacgcgaatc gcttcgctgt cacccaccgg
     1321 tacgtagagg aacagacgca tcaccacacc gaagatagcg attttgctcg ccgtcgccag
     1381 gaaagtggaa accggcgcag gcgcgccctg gtatacgtct ggcgtccaca ggtggaacgg
     1441 caccagcgag agtttgaagc cgaggccaac aatcatcagt ccgaaacctg ccagcaacag
     1501 cggctcgttg agcataccgt cgccgaggtt tttacccagc gcgacaaacg acaggtcgcc
     1561 agactgtgca tacaccagcg ccataccaaa cagcaggaaa gaagacgctg cggcagaaag
     1621 gatggtgtat ttgatactgg cttccagcga acgtttctgg cggaaagcgt aaccgaccag
     1681 gccaaacagc ggcaaagaga tcagttcaat accgaggaac aaagacgcca gatggttggc
     1741 attcgccagc aggatcccgc ccagcgcggc aattaacacc aacaggtaga actcatcctt
     1801 gttgtcgtta tagccttcaa gccacgggta ggcgaaagta caggtggcga ggctcgccaa
     1861 caataccagc ccggtgtaaa gcatggcgaa accatcaacg cgcatcagcg gcgtaacgtc
     1921 catagcgccc gcctggccga caaaccagag cgaaaccagc gccgcgttaa gcccaataac
     1981 cgagagcgta gcgttgagga aatgattgcg tcgccacgca atggagagca tcacaaccac
     2041 caccgtcaag ccgacgatca gcaacggtag cagtgcgatc aggttttgtg gagttattgt
     2101 catggcgatt tacggccttg tagtagtaac ggaattaaca aaccactgct ggatattgcc
     2161 aatcgcggag tgcgaggtat ccagaatcgg ctgcggatag aagcccagca gtaccagcag
     2221 caccaccagc aacaggatca taaacagctc acgcagcgac atccctggca gttcctggct
     2281 ggcaatctgg cttttcgctt taccgaagta agcgcgatgc aacatcgcca gcgaataaac
     2341 agatgcaaag accagcccaa aggtagagat aacggtaatc accgggacaa cctggaagct
     2401 gccgaacaga atcataaatt cgccgacgaa gttaccggtg ccaggcatcc caagcgttgc
     2461 caccgcaaag aacagcgaca gtgctggcag ccatttcatc ttgctccaca gaccgcccat
     2521 catgcgcatg tcgcgggtat ggatacgttc ataaagctga ccacaaagaa taaacagacc
     2581 cgccgccgac aagccgtgcg caatcatctg gattaccgcg ccctggtagg ccaactggct
     2641 gccggtgtag atagcaatca gcacgaagcc catgtgggaa accgaggtgt aggcgatcag
     2701 acgtttgata tcggtctggg cgaaagccat ccacgcaccg tagaagatgc cgataacccc
     2761 cagccacata gcgattggcg caaactctgc cgatgcgttc gggaacagcg gcagggagaa
     2821 acgcagcaaa ccgtaagcgg cagttttcag caagatcccc gcgaggtcaa cggaaccggc
     2881 ggtcggagcc tgagagtgcg catccggtag ccagccatgc agcggaacca ccggcatttt
     2941 gactgcgaag gcgatgaaga agcccagcat taacaggtat tccacaccac tggacattgg
     3001 cgtattcagc agctcttcat agttgaaggt ccagacgccg gtcgcattgt agtgaacaaa
     3061 aaccagcgcc aggatggcaa tcaacatcac caggccactc gcctgggtgt aaatgaagaa
     3121 cttggttgcc gccgtgatac gcgttttacc gtcagaggct ttatgccccc acagtgcgat
     3181 caggaagtac atcggcacca gcatcatttc ccagaagaag aagaacagga acatgtcgat
     3241 ggcaaggaac acgccgataa cgccgcccag gatccacatc aggttgaggt ggaagaagcc
     3301 ctgatatttt tcgatctctt tccacgaaca tagtaccgcc agcacaccga gcagaccggt
     3361 cagcacgacc atcagcagcg acagcccgtc aatggcgaga tgaatagaga taccaaaacg
     3421 cgggatccac ggcatgtcga attcagactg ccactgcgga attccggcgg attgcgtcag
     3481 tgaataaccg ccctgcaacc acagttgcag cgacagcgcc agcgtcaatc ccatggtgat
     3541 cagcgcgatc cagcgtggca ccttgacgcc aaagcgttcg gtctgccagc acaggaagcc
     3601 gccaataaag ggaattaata ttagccaggg tagtaacatg gcgatcttta ttccttgtaa
     3661 aagtcccgtc aggaccggat tttcaacgaa ttctcacgac agaaaattct cttcggactg
     3721 ggggtagtgt cggatgcacc gcgaaggcgg cttatccgac ctacgttctt atatgcccag
     3781 gggcaatccc acaatcctta actcaacgca gtaccatcaa cagtgccagc accacgaccg
     3841 caccgatgct catggatgcc acataccagc gcagataacc gttctcgctt aacagcagac
     3901 ctttacctgc aaagcgggaa agaaccgccg ggatgttcat cattgagttc agcggatcgc
     3961 gtttcagcaa ccaggcaata cccaggaacg gtttgacgaa cactttgtcg tacagccagt
     4021 caaagcccca ggcgttgtac caccaggtgc ccagcagacg gcccggcgca ctgttggcaa
     4081 tggaggtcac cagagtacgt ttacccagcc acagccaggc tgccagcaga atgccgacca
     4141 ccgcgaccac gccagaggta atttccaggg tcaacatgct gccgtgcgcc agctccgtcg
     4201 tttgcggaag cacgccctgc agcggcggta caatcagtgc gccaacgaag gtggaaagga
     4261 tcagcagcac aatcagcggc aggctgtgag ttaccccttt cacggcgtga gcgtgaattt
     4321 gttcttttcc gtggaagacg atgaaaatca tacggaaggt gtagagcgag gtcataaacg
     4381 caccgaccag acctgccacc atcagattga tatgaccatt cgccatcgca cccgcgagga
     4441 tctcatcctt actgaagaag ccagcagtga ccagcggcag cgccgacagt gctgcgccgc
     4501 ctaccaggaa gcagagataa accagcggaa tagatttacg cagaccgccc atcttgaaga
     4561 tgttctgttc gtgatggcag gccagaatga cggaaccgga tgccaggaac agcagcgctt
     4621 taaagaacgc gtgagtcatc aagtggaaaa tcgccgcatc ccatgcctgc acgccaagcg
     4681 cgaggaacat gtagccaatc tggctcatgg tagagtaagc gagaacacgt ttgatgtctg
     4741 tctgtaccag cgcggcaaaa ccggccagca gcagcgtaac cgccccgaca atacccacca
     4801 gatgcagaac ttccggggtc atcaggaaca ggccgtgggt acgggcgatc aggtagacac
     4861 ccgcggttac catcgttgca gcgtggatca gcgcggagac aggcgtcggg ccagccatcg
     4921 catcggcaag ccatgtctgc aacggcaact gcgcagattt accgaccgca ccgcccagca
     4981 gcatcagcgt cgcccacatc agcatgttat tgccgtcagc aaagtgcgct ggtgccagtt
     5041 ccaccatttc gcggaagttc agggtgccca gttcgttgta aagaatgaac agtgcgaaag
     5101 cgaggaacac gtcacccaca cgggtcacga cgaacgcttt cattgccgct gcgccattct
     5161 tcggatcggt gtaatagaaa ccgatcagca gataggagca caggcccacg ccttcccagc
     5221 cgaggtacat cagcagcagg ttgtcggcaa gcaccagaac taccatgctg gcgatgaaca
     5281 ggttggtgta ggcgaagaag cgagagtagc cctcttcacc gcgcatgtac caggaggcgt
     5341 acatgtgaat aaggaaaccc acaccagtga ccaccgagag catggtcagc gacaggccgt
     5401 ccagcaccag attgaaaccg atgttaaagt cgcctaccga catccacgtc cacagcggct
     5461 ggctgtatga ctgctcgccg ttagcgaaga aatcaacgcc gataaaggcg gttaccagcg
     5521 ccgccaggcc cacagagcca acgccgacga tcgccgaaac gttttcagac cagcgcccac
     5581 gggagaacgc cagcaggacg aagccaatca atggcaaaat aatggttaag gcaagcatgt
     5641 tcatccgcgc atctcactta ctgaatcgat gttcaggttc tggcgacgac ggtgaagttg
     5701 cagcagcagc gcaaggccga tactcgcttc tgccgccgcg aggctgatgg cgagaatgta
     5761 catcacctga ccgtcggtct gaccccagta gcttccggcg accacaaagg ccagcgcgga
     5821 ggcgttaatc atgatttcca gaccaatcag cataaacagc agattgcgac ggataaccag
     5881 accggttaag ccaagaacga ataagattgc cgcgaggatc agtccatgtt gtaaggggat
     5941 catgcgtgct cctccgtttt tcttttcgcg ctgtcgtctt tacgattgct cagcacttca
     6001 cccgcacgct cttcacgacc gacgtggaag gccacaacca gacccgcgag cagcagcata
     6061 gaagccagtt ccaccgccag tacgtaaggc ccgaacagcg taatacccac tgctttagca
     6121 ctgattggcg taccgtcgat accctgatca ttaacaccaa ggatggcgta aacaatcacc
     6181 accagcatga tggccgacaa aattgccgga ccaatccaca cctgcggttt cagccactgg
     6241 cgttcctgtt cgatttctga accgcccagg ttgagcatca tcaccacgaa cacgaacagc
     6301 accataatgg cacccgcgta gacgataatt tccagcgcac cggcgaagta agcgcccagt
     6361 gagaagaaca ccccggaaat cgccagcagc gaaataatca ggtacagcag tgcgtgtacc
     6421 ggattggtat gggtgatcac tcgcaaggtc gcaagtatgg ctatcaggcc acagatataa
     6481 aaagcgaact ccattgcccc tctccttacg gtaacaggct cttgacgtcg ataggcttgg
     6541 cttcgttctc tgcttcgccc ttatctttgc cgtcgattgc catacctgcc atccggtaga
     6601 agttatattc cgggtatttg cccggaccgg agatcagcag atcctctttt tcgtaaacca
     6661 gatcctggcg cttgtattcc cccatttcga aatccggggt taactgaatc gccgtggtcg
     6721 gacaggcttc ttcgcacaga ccacagaaaa tgcagcgtga gaagttgatg cggaaaaatt
     6781 ccgggtacca gcgaccgtct ttggtttctg ctttttgcag cgagatacag ccgaccgggc
     6841 aggctaccgc gcagaggtta caggctacgc aacgctcttc gccgtccggg tcgcgggtca
     6901 gaacgatacg accacgataa cggggcggca gatagaccgg ctcttccggg tacattcgcg
     6961 tttcgcgttt ggcgaacgcg tgcaggccga tcatccagat actacgaacc tgggtgccga
     7021 aacctactaa caattctttt aaggtcatgg tcttattgcc ccttattgcg cctgccagag
     7081 aatgacagcc gccgttacca gcaagttgat cagcgtcagc ggcaggcaga ttttccagcc
     7141 gaaggacatt acctggtcat aacgcggacg cggtaacgac gcacgaatca aaatgaacat
     7201 catcataaag aacgcggttt tcagcgcgaa ccagatgaat ggcggtaaca acgggccttg
     7261 ccagccaccg aagaacagcg tcaccatcaa tgcagagatg gtcacaatcc cgatgtactc
     7321 acccacgaag aacagaccga acttcatacc ggaatattca atgtggtaac catccgccag
     7381 ttcctgctcg gcttccggct ggtcaaacgg gtgacggtga cataccgcca cgcccgcgat
     7441 ggcaaaggta ataaaaccaa agaattgcgg gataacgttc cacacatgcg cctggctgtt
     7501 gacgatgtcg gtcatgttga atgaaccggc ctgcgccacc acgcccatca gggaaagccc
     7561 gaggaacact tcgtagctca gggtctgcgc agaagcacgc atcgcaccca gcaacgagta
     7621 tttgttgtta cttgaccagc ccgcaaacag caccgcgtaa accgccagac ctgccatcat
     7681 caggaagaac aaaatcccga tgttcaggtc ggcaaccacc caacccggac tgactggcac
     7741 aatcgcaaag gccagcagca gcgaggtaaa ggcaatcatc ggtgccaggg taaagatgac
     7801 gcgatccgag aatttcggga tccagtcttc tttaaagaac attttgatca tgtccgcaac
     7861 cagctggagc gaaccgcccc agccaacacg gttaggtccg taacggttct ggaacagacc
     7921 cagcaggcga cgttcgccaa agctcatgaa tgccccgcag gtgacaacca ccagcaggat
     7981 caccaccgct ttgaggatgg tcagcaggat ctcaatcagt tccggtgata tccaactcat
     8041 tgttgtgcct ccttgagatc ctcaagatgc gcgccagcca gcaccggagc aataccggac
     8101 atacccatcg gcaagcccac ctgccctgcc gtcagccctt cagcgatttc aaccggcagc
     8161 gtgaccgtgt tgccatcgta actaaaggag acgtgtgtac ctgcgttcac acctaacttc
     8221 gcggcatccg ctgggttgag tttgatgtac ggctgcggca tacggctctg gaagaccgga
     8281 gcacgctgtg acaattcatc gctgccaaac aggtggtaat acggcgcgat acgccatttc
     8341 ccgtcctgcg gctggaagcg tgccggtacg ctggtgaagt aatccagacc attttcgctg
     8401 gtttcaaaca gacgcacgcc cggatcgcca aagcgcagtt tgccgcccac ttcgtcctgg
     8461 aatttgttcc acgcctgcgg ggagttccag cccggcgccc aggcaaacgg cacttgcgaa
     8521 cggtgcgcag tcggctggtt gttaccttcc atcgagaagg tgaacatggt gtcaatatcc
     8581 tgcggctgac gcggctcatg aacgctgata ttggcgcgca tggcggtacg accgctgtaa
     8641 cggtgcggtt cacgggccag tttctgccca cgaatacgga atgtcgcatc cggcgcagca
     8701 tctttgatac ctgccagttc cgggattttc gccacaacag cgtcaatcac atggtcgagc
     8761 tgcgtccagt ccacttcacg gctcagcagg gtgctgtgca gcgagtgtaa ccagcgccag
     8821 ctttccagca tgacagtttt gctgtcgtaa taagcaggat cgtaaacctg gaagaaacgt
     8881 tgggcgcggc cttcgttgtt gatcaccgta ccgtcgcttt cagcaaagct ggcagcagaa
     8941 agtaccagat gggcgttttc cataatcgct gtgcgttgat gatcaaccac catcaccagc
     9001 ggcgctttag ccagcgcagc attcacgcgg gtagcggaag cgtgacgatg cagatcgttt
     9061 tccagcacca ccaccgcgtc ggcgcgtccg gtttccagtt cggtcaacgc ttcttcaagc
     9121 gaaccgccac ccataatgcc cagccccatg ctgttgacgg aacgggcaat catggtgata
     9181 ccaacgtcag cgccgcgacc tttcagggct ttcgcgacgt tagccgcagc ctgaatcacc
     9241 tctatgctac cggcgttcgt cccggagata atcaacggtt tcttcgcacc tgccagtgcc
     9301 tgcacgatga cgtcgatttt gctttgcagc tcaggttcga taccgtcaac cgctggcgca
     9361 gagttatcca gcgcatgggc gatggcaaaa cctaaacgcg cctgatcttc aaccggtgcg
     9421 cggtaagtcc acgccgcgat atcatccaga cgggtgtcat caacgttggt aacaaacagc
     9481 ggatgcttcg cacgttgacc gatgttgagg attgccgcaa tctgccagtc agccactttc
     9541 tgtgctgccg ccatttcgcg cgctttacct ttcacagcct gacgcactgc cagcgcgacg
     9601 cgcgcgccgg tctgggtaac gtcttcgccc agcaccagta ccgcatcgta agattcgatt
     9661 tcgcgcagag ccggagtata aatgccgcct tcacgcagca ctttcagcgc cagttgcaga
     9721 tgttcctgct caccgtgagc gataccggtg tagaagtttt cttcgcccac cagttcacgc
     9781 agcgcaaagt tgctttccac gctggcacgc ggagaaccaa taccgatcac tttcttcgac
     9841 tgacgcagaa tatctgccgc gccctgcatt gcctgttcgg cgttgagggt aatgaaatca
     9901 tcgccacgac gctgtactgg ttgacgcgga cgatctttca ggttgacgta accgtaaccg
     9961 aaacgaccac ggtcgcagag gaagtagtgg tttaccgtac cgttgtaacg gttttcgata
    10021 cgacgcagtt cgccgtaacg ttcaccgggg ctgatgttac agccgatgga acattgctgg
    10081 cagatgctcg gcgcaaactg catatcccat ttacggttgt aacgctcgga gtgcgttttg
    10141 tcggtaaata cgccggtcgg gcaaatttcg accaggttac cggaaaattc gctttccagc
    10201 gtgccgtctt ccgggcgacc gaagtagacg ttgtcgtgcg caccgtaaac gcccagatcc
    10261 gtaccgtcag cgtaatcttt gtagtaacgc acgcagcggt agcaggcgat gcagcggttc
    10321 atttcgtgag agatgaatgg ccccaaatcc tgattacggt gggtacgttt ggtgaaacgg
    10381 tagcgacgga agctgtgtcc ggtcatcacg gtcatatcct gaagatggca gttaccgcct
    10441 tcttcacata ccggacagtc gtgcgggtgg ttggtcatta accactcgac cacactttca
    10501 cggaactgtt tcgcttcttc gtcgtcaatg gaaataaagg tgccatcgga agccggtgtc
    10561 atacaggaca tcaccaggcg accacgcgtg tcttccgcgt tttggtattg cttcaccgca
    10621 cactggcggc aagcaccgac gcttcccagc gccggatgcc agcaaaagta aggaatatca
    10681 aggcccagag acagacaagc ttccagcagg ttgtccgctc cgttgacctc gtattctttg
    10741 ccgtctacat gaattgtagc cattagcatg cttccagttt tctcggtcag ggaccgagcg
    10801 ttaatcgaaa ttcggttacc agcgctcttt cagcaggttc ggctgaatcc cattaatcaa
    10861 atgggtattg ctgaacggct gtttgattcc cgcctcaaat tcttcgcgga aatatttgat
    10921 ggcgctctgt aacggctcca ctgcaccagg tgcgtgggca cagaaagttt tacccgggcc
    10981 taagaatcga cacagttgct caagtgtttc gatatcgccc ggctgacctt caccacgctc
    11041 cagcgcacgc agaattttca cgctccacgg cagaccgtcg cggcacggcg tacaccagcc
    11101 gcaggactca cgggcgaaaa actcttccag gttacgcacc agcgacacca tgttgatctc
    11161 atggtcaacc gccatcgcca gcgccgtacc cagacggctt cccgctttac cgatactttc
    11221 gaattccatc ggcaggtcaa ggtgcgcttc ggtcaggaag tcggtgcccg cgccgcctgg
    11281 ctgccaggct ttgaatttca gaccgtcacg cataccaccg gcgtaatctt cgaggatctc
    11341 gcgcgcggta gtaccaaacg gcagttccca cagtcccgga tttttcaccc gaccggagaa
    11401 gcccatcagc ttggtgccag catctttact tttcgagatg ttctgatacc actccacgcc
    11461 gttagcgagg atcgccggaa cgttacacag ggtttcgacg ttgttgacac aggtcggttt
    11521 gccccatacg ccggaggttg ccgggaaggg gggtttcgag cgtgggttag cacgacgccc
    11581 ttccagggag ttgattaacg ctgtttcttc cccgcagatg tagcgccctg ccccggtatg
    11641 gacgaacagt tcgaagtcga aacctgttcc cataatgttt ttgccaagca agcccgcttc
    11701 agtggcttcg gcaatggcgc ggcgcagatt tactgccgct tcgatatatt cgccacgcag
    11761 gaagatgtag ccacggtaag ctttcagcgc aaacgcggag atgagcatac cttccaccag
    11821 caggtgcggc agttgctcca tcagcaggcg gtctttatag gtgcctggct ccatttcatc
    11881 ggcgttacac agcaggtaac ggatgttcat ggattcgtct ttcggcatca ggctccattt
    11941 caggccggta gaaaagcccg cgccaccgcg ccctttcaga ccagcgtctt ttacctgatt
    12001 aacgatttcg tccggagaca gcccggtcag cgccttacgc gcgccttcgt aaccgttttt
    12061 gctgcgatat tcgtccagcc acactggctg tttgtcatcg cgcagacgcc aggtcagcgg
    12121 atgcgtttcg ggagtacgga taatgttttt catttatacc gctccagcag ttcagggatc
    12181 gcttccgggg tcagatgcgc gtgagtatcc tcatcgatca tcatgtttgg ccctttatca
    12241 cagttcccca ggcagcaagt tggaagcagc gtaaagcgac cgtcaaatgt cgtttgccct
    12301 ggtttgatgt ttagcttttt ctcgagcgcc gcctgaatac cctggtaacc gttgatatga
    12361 cagaccacgc tgtcgcaata acggatcaca tggcggccaa ccggctggcg gaagatctgg
    12421 ctgtagaacg ttgccacacc ttcgacgtcg cttgccggaa tacccagcac atcggcgatc
    12481 gcgtggatcg caccatccgg cacccagcca cgctgcttct gaacgatttt cagcgcttca
    12541 atggacgccg cacgcgggtc ttcgtagtgg tgcatctcgt gctcaatcgc ttcacgctct
    12601 gccgcactca gctcaaaagc ctcggtttgt ggttgttgat tctcgtgcat aattagcggt
    12661 ccacatctga cataacaaaa tcgatactgc ccagataaac aatcaggtca gacaccaggc
    12721 tgccgcggat cgccgccgga atttgctgca aatgcgcata actcggcgtg cggatacgag
    12781 tgcgataact catggtgcta ccgtcgctgg tcaggtagta actgttgatc cccttggtcg
    12841 cctcaatcat ctggaaagat tcattggcag gcatcaccgg cccccacgac acttgcagga
    12901 agtgggtgat cagggtttcg atatgttgca gcgtgcgctc tttcggcggc ggcgtggtca
    12961 gcgggtgatc cgctttgaac gggccttccg gcatgttgtt gaggcactgc tcaagaatgc
    13021 gcagactctg gcgcagctct tccactttca gcattacgcg ggtgtagcag tcagaaacac
    13081 caccaccaac cgggatttca aagtcgaagt tttcatagcc agaataagga cgcgccttac
    13141 gcacgtcgaa gtcgatcccg gtagcacgca ggcccgcgcc agtggtgccc cactccagcg
    13201 cctctttcgc gccataggcg gcaacgccct gggaacgacc tttcagaatg gtattttgca
    13261 gcgccgcttt ctcgtaagac gccagacgtt tcggcatcca gtcgaggaac tcacgcagca
    13321 ggcgatccca gccgcgcggc aggtcgtgcg ctacgccgcc aatacggaac cacgccgggt
    13381 gcatacggaa acccgtgatc gcttccacca gatcgtaaat tttctgacga tcggtaaagg
    13441 cgaagaacac gggcgtcatt gcgccgacgt cctgaataaa ggtcgagatg tacagcaggt
    13501 ggctgttgat acggaacagt tcggagagca taacgcgaat gacgttaacg cgatccggca
    13561 cggtgatccc ggccagtttc tctaccgcca gcacgtaagg catttcgtta acgcagccgc
    13621 cgaggtattc aatacggtcg gtatacggaa tgtagctgtg ccaggactgg cgctcgccca
    13681 ttttctccgc accacggtgg tggtagccga tgtctggtac gcagtcgaca atctcttcgc
    13741 cgtcgagttg taaaacgata cggaaagccc cgtgcgccga cgggtggttc ggaccgaggt
    13801 tgaggaacat gaagtcctcg ttttcggtgc cgcgcttcat cccccactct tccggtttga
    13861 aggtcagggc ttccatctcc agatcctgtt tggctttggt cagctcaaac ggcgagaatt
    13921 cggtagcgcg cgccgggtaa tctttacgca gcgggtgacc tttccaggtt tgcggcatca
    13981 tgatgcggcg caggttcggg tgaccgtcga aagtaatgcc aaacagatcc caggtttcac
    14041 gctcatacca gttagcgttc gggaacagtt tggtgaaggt cggtacgtgc aggtcgtttt
    14101 ctgccagcgc caccttcagc atgatgtcgc ggttacgatc gatagaaatc agatggtaga
    14161 aaacggaaaa atccgcggca ggtaaccctt cgcggtgtgt gcgcagacgt tcgtccatgc
    14221 cgtgtaagtc aaacagcatg acgtaaggtt tcggcagttt ctttaagaaa tcgccaactt
    14281 ccagtaattg ttcacgcttg atccacacaa cgggaacccc ggtgcgagtc gcctgaacag
    14341 taaaggcatc cggcccaaaa cggttgcgca gttcgccaat caccggatca tcaagatgat
    14401 cgcgggtctg ccaggcgggt tcttgcgcgg ttaagtcggt catattgttc accattgcaa
    14461 atggtccgtg gtgactgtcg gcggagtaag cttcgcgaaa tagatttagg aataagcgaa
    14521 gaaaaatccc tttgccgaca ggcgcaaatt aaatctcgtc aggtgtacgc aggttggtta
    14581 cggcaatgcg ttcaccgcgc ttgcgttcgc gctctgattg catattggcg cgataaacgc
    14641 cctgatcgcc aaccacccag gagagcggac gacgttcttt gccgatagat tcctgcaaca
    14701 gcatcagtgc ctgcatgtac gcttcaggac gcggcgggca gcccgggata tacacatcaa
    14761 ccgggatgaa tttatcgacg ccctgcacaa cggaataaat atcgtacata ccgccggagt
    14821 tggcacaggc acccattgag ataacccatt ttggttccag catctggtca tacagacgct
    14881 gaataaccgg tgccattttg gtaaagcagg ttcctgcaac caccatcagg tcagcctgac
    14941 gcggcgaagc acgcaatact tctgcgccaa aacgcgccac gtcatgcacc gcggtaaacg
    15001 aagtcaccat ctcaacgtaa cagcaggaaa gaccgaagtt atacggccaa attgagtttt
    15061 tacgacccca gttaaccatg tcattgagct tgcccataaa cacgtttttg ttaacttctt
    15121 gctccagagg gtcggttacg atctcctgct tttgcagggg gtaacggtcg ttctcaccgt
    15181 tgggatctat gcgggtgagc gtataatcca tcttaatgcc tcgcggttag cgttgacgat
    15241 tagcgatact gttcgtttcc gggttcatac gctcgcggcg tgaacgcgcg ggcgtccagt
    15301 ccagcgcgcc aatacgcacc agataaacca gacctgccag taacacaaaa ataaaaattg
    15361 cagcttccac aaagcctacc cagccgcttt cgcggataga ggttgaccat gcgaacagat
    15421 acagcgcttc aacgtcgaag ataacgaaga acatggccac cagataaaac ttggcggaca
    15481 ggcgtaagcg ggcggagccg accgagtcga taccggattc aaacggcacg tttttcgacc
    15541 tcgcgcgtgc gcgaccgcct aaaaaccaac cgcctaccag catcaggcaa cacaggccaa
    15601 tggcaacgat aagaaagata gcgaatgccc agtgatgagc gatgacttca gtggatgttg
    15661 acatactcat tgcttactca tcaaaagtag cgccagattc actgctcttc acggcagatg
    15721 gacgccacat cgattcatgg ggaggaataa aaaaaacctt acaatcactg tagaaattct
    15781 tttatacagc taattgatgt ggtcttttac tcctttctat aaccttttgt caactttaac
    15841 aaaagtttct tcacattagt ttacataata tcaacaccat tagcatttaa tgccctttca
    15901 ccccagatcc ttgacgactc caggataatt agatgttgtt gaatcgtgtc cgttgtgaag
    15961 caatggaaaa aatacgggtc tattttgaca ggaatttgtg tcgattcctc cccccaaaag
    16021 agagtatttt cttgatctgt gacacgcttt tgtcattcca taacaaaaac gcagcaacaa
    16081 atttacgtat tttttaacat cattgtagca ggtgattttt ttcaggcgat tatttgtgcg
    16141 ttcgggacgt gaatctctgg tgattgaaaa atgaacagtt ttgtacgttc tgcactatgc
    16201 gaggaaggat ttttactaaa aaaaagccgc tggggtttaa aacaccccca gcggctcgtt
    16261 ttttacacta ttgtctcagg aattatctat cgtccgtcga ttactcgata tccctttcaa
    16321 tcaacaatga atcatcccct tccggagcag acattgggct gtattgccac ggattatggt
    16381 agctctccat cgcctgatag atcacctggg ccagctcatt attactggac gggtcatagc
    16441 acagcaaata ttcggtatca ggtagcggcg gtaagccatc tactccactc agcacacgca
    16501 ggtccgggct catcatctca accggccttg ccgtcacgcc aagaccggct ttcactgccg
    16561 cacgaacggc cggaagcgtc gaggcgacat aagccagtcg ccatggaata tctgctttat
    16621 taagcgtcgc cagcaccata tcgcgaaacg gacttggatc atccagcaat acaagaggga
    16681 tcggctcgcc tttttgcaat acgtattccg ctgcgcagta ccagtgtgtt ggcgaggtac
    16741 gcaggttcaa tgccttaaac gctgaaggac gatgggtggt taccatcaaa tccacttcct
    16801 gggattccag catttctgcc atataggcat tacgtttaac gcggacatcc agcgctaatt
    16861 tcggataaac cgaactcacg cgatttaaca ggaaaggtaa gatcgtatcg gcagattcat
    16921 ctgaagcacc gatagttaac acgccctgaa gattactgaa cattaatgat gagcaggcct
    16981 catcattaaa acgcaggatt ttcctggcgt aaccaagaag ttgaatgcca tgttcagtta
    17041 acagtttgtt gcgaccgtga cgagcgaaca gttctttccc aacgagttgt tccagacgct
    17101 gcatttgctg acttacggcg gactgagtac gacacacagc ggcagctgcg gcagcaaaag
    17161 tgttcagatc ggcaacagca acaaatgttc tcagcagatc gaggtcgagg ttaattatcg
    17221 gacgatttgc acttatcata tattatcact tactggcggc tcatactgag ctggttaatg
    17281 ctgtgcacac acaaacaagc aattccattt gtaatgtgcc tccctggcag tttcatccgg
    17341 aacccggacg aaagtaaaaa tgcatatgag ttgcactaaa aaagcgactc acattgttcc
    17401 gttataatgc ctgaagtaga tcacagaata tatcttcagg gatcgcatat ctattaagtt
    17461 actcactctt ttctatttat gacatgcgcg tgtttgtata aatgtaaatg tgagtccttg
    17521 ttccactctc gtgcagcatc gctggtcata cgcgaacacg tacaaacagc aatggtgagg
    17581 cggcatcaag agcagggatc cgtcatttat gcgagcattt taccccaaaa accttttatt
    17641 tataagggtc attgcgaatt atctgatgca aagttatg
//
LOCUS       DBANUU010000090        17566 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000090 DBANUU010000000
VERSION     DBANUU010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.090"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            61..1200
                     /gene="arnB"
                     /locus_tag="ACIE68_003120"
     CDS             61..1200
                     /gene="arnB"
                     /locus_tag="ACIE68_003120"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFX5909395.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPDWPHVHAWHLFIIRVDEQCCGISRNALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1204..2172
                     /gene="arnC"
                     /locus_tag="ACIE68_003121"
     CDS             1204..2172
                     /gene="arnC"
                     /locus_tag="ACIE68_003121"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFX5909396.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2172..4154
                     /gene="arnA"
                     /locus_tag="ACIE68_003122"
     CDS             2172..4154
                     /gene="arnA"
                     /locus_tag="ACIE68_003122"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFX5909397.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFTYVGNQKFTVWSSRVHPHASKAQPGSVISIAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4151..5041
                     /gene="arnD"
                     /locus_tag="ACIE68_003123"
     CDS             4151..5041
                     /gene="arnD"
                     /locus_tag="ACIE68_003123"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFX5909398.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5041..6693
                     /gene="arnT"
                     /locus_tag="ACIE68_003124"
     CDS             5041..6693
                     /gene="arnT"
                     /locus_tag="ACIE68_003124"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFX5909399.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPNFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFEIYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6690..7025
                     /gene="arnE"
                     /locus_tag="ACIE68_003125"
     CDS             6690..7025
                     /gene="arnE"
                     /locus_tag="ACIE68_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFX5909400.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7025..7411
                     /gene="arnF"
                     /locus_tag="ACIE68_003126"
     CDS             7025..7411
                     /gene="arnF"
                     /locus_tag="ACIE68_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFX5909401.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPMTKQRY"
     gene            complement(7405..7671)
                     /gene="pmrD"
                     /locus_tag="ACIE68_003127"
     CDS             complement(7405..7671)
                     /gene="pmrD"
                     /locus_tag="ACIE68_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFX5909402.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(7781..9136)
                     /gene="menE"
                     /locus_tag="ACIE68_003128"
     CDS             complement(7781..9136)
                     /gene="menE"
                     /locus_tag="ACIE68_003128"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFX5909403.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLASFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9133..10095)
                     /gene="menC"
                     /locus_tag="ACIE68_003129"
     CDS             complement(9133..10095)
                     /gene="menC"
                     /locus_tag="ACIE68_003129"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFX5909404.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMTSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10095..10952)
                     /gene="menB"
                     /locus_tag="ACIE68_003130"
     CDS             complement(10095..10952)
                     /gene="menB"
                     /locus_tag="ACIE68_003130"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFX5909405.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10967..11725)
                     /gene="menH"
                     /locus_tag="ACIE68_003131"
     CDS             complement(10967..11725)
                     /gene="menH"
                     /locus_tag="ACIE68_003131"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFX5909406.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLTG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVVLRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11722..13392)
                     /gene="menD"
                     /locus_tag="ACIE68_003132"
     CDS             complement(11722..13392)
                     /gene="menD"
                     /locus_tag="ACIE68_003132"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFX5909407.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13481..14776)
                     /gene="menF"
                     /locus_tag="ACIE68_003133"
     CDS             complement(13481..14776)
                     /gene="menF"
                     /locus_tag="ACIE68_003133"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFX5909408.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNRRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14855..15160)
                     /gene="elaB"
                     /locus_tag="ACIE68_003134"
     CDS             complement(14855..15160)
                     /gene="elaB"
                     /locus_tag="ACIE68_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFX5909409.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDTADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15215..15676)
                     /locus_tag="ACIE68_003135"
     CDS             complement(15215..15676)
                     /locus_tag="ACIE68_003135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFX5909410.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15741..16658
                     /gene="rbn"
                     /locus_tag="ACIE68_003136"
     CDS             15741..16658
                     /gene="rbn"
                     /locus_tag="ACIE68_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFX5909411.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16846..>17566
                     /locus_tag="ACIE68_003137"
     CDS             16846..>17566
                     /locus_tag="ACIE68_003137"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
                     /protein_id="HFX5909412.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SHLKPTGPDNSDVLLFKKLFPPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDDT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPNEVQLFHINTGGHWILVSL"
BASE COUNT         4139 a         4729 c         4418 g         4280 t
ORIGIN      
        1 acacaacact ctttatagca aatataagtg gacaggtatt caatggcgga aggaaaagca
       61 atgtcagaat ttttgccttt ttcgcgacca gcaatgggcg tggaggaact cgctgcagtt
      121 aaagaggttc tcgaatccgg ttggatcaca accggtccga agaatcaggc gcttgagcaa
      181 gccttttgcc agttgacggg aaatcagcat gccatcgcgg tcagttcagc caccgccgga
      241 atgcatatca cgctaatggc gttggaaatt ggcaagggcg atgaagtgat tacgccttcc
      301 ctgacctggg tttcaaccct caatatgatt tccttgttgg gtgcaacgcc ggtaatggtg
      361 gatgtcgacc gcgatacgct gatggtcacg cctgaagcga tcgagtcagc cattacgcca
      421 cgcactaaag ccatcattcc ggtgcattat gccggtgcgc cagcagatat tgacgccatt
      481 cgcgccattg gcgaacgtta cggcatcgca gttatcgaag atgctgccca tgccgtcggt
      541 acgtattaca aagggcgaca tattggcgca aaaggtaccg ctattttttc atttcatgcc
      601 attaaaaata ttacctgtgc ggaaggtggc ctgattgtaa ctgataatga aaaccttgcc
      661 cgccagctac ggatgctgaa atttcacggt ctgggtgtcg atgcctatga cagacaaacc
      721 tggggccgtg caccgcaggc tgaagtctta acaccgggct ataagtacaa tctgaccgat
      781 attaacgccg cgattgccct gacacagtta gtcaaattag agcacctcaa cacccgtcgg
      841 cgcgaaattg cccagcaata tcagcaagca ctggcagctc tcccctttca gccattaagc
      901 cttcccgact ggccgcacgt tcacgcctgg catctgttta ttattcgtgt cgatgaacaa
      961 tgttgtggta tcagtcgaaa tgcgttgatg gaagcgttaa aagaaagagg cattggtacc
     1021 gggttacatt tccgcgccgc tcacacacaa aaatattatc gcgagcgttt tcccacgctg
     1081 tcgttaccga ataccgaatg gaatagcgaa cgcatctgtt cgttgccgct gttcccggat
     1141 atgactaccg ccgatgccga ccgcgtcatc acagcccttc agcaactcgc aggacaataa
     1201 gccatgtttg aaatccaccc tgttaagaaa gtctcggtgg ttattcccgt ttataacgag
     1261 caggaaagct taccggaatt aatcaggcgc accaccacag cctgtgaatc gttggggaaa
     1321 gagtatgaga tcctgctgat tgatgacggc agtagcgata attccgcgca tatgctggtc
     1381 gaagcctcac aagcggagaa cagccatatt gtgtctattt tgcttaaccg caattacggg
     1441 caacattcag cgattatggc gggattcagt cacgttactg gcgacttaat tattaccctt
     1501 gatgccgatc tccagaatcc gccagaagaa atcccccgcc tggtggcaaa agccgatgaa
     1561 ggttacgacg tggttgggac tgtacgccag aaccgccagg acagctggtt tcgtaaaacc
     1621 gcttcgaaga tgattaaccg gcttattcag cgcaccactg gcaaagcgat gggtgactac
     1681 ggttgtatgc tgcgcgccta tcgccgtcat attgtcgatg cgatgttgca ctgccatgaa
     1741 cgcagcacct ttatcccgat tctggcgaat atcttcgccc gccgtgcaat tgaaattcca
     1801 gtacatcatg ccgagcgtga gtttggtgaa tccaaataca gttttatgcg cctgattaat
     1861 ttgatgtacg acctggtgac ctgccttacc acaacgccgc tacgtatgct gagtctgctc
     1921 ggtagcatta ttgcgattgg aggttttagc attgcggtgc tgctggtgat tttacgcctg
     1981 accttcggac cacaatgggc ggcagaaggc gtctttatgc tatttgccgt gctgtttact
     2041 tttattggcg ctcagtttat cggcatggga ttactcggtg aatatatcgg caggatctac
     2101 accgatgtcc gcgcccgccc ccgctatttt gttcagcaag ttatccgtcc atccagcaag
     2161 gaaaatgaat aatgaaaacc gtcgtttttg cctaccacga tatgggatgc ctcggtattg
     2221 aagctctgct ggctgccggt tacgaaatta gcgccatttt tacccatacc gataatcccg
     2281 gtgaaaaagc cttttatggt tcggtggctc atctggcggc ggaaagaggc attccggttt
     2341 atgcgccgga taacgttaat catccgctgt gggtggaacg cattgcccaa ctgtcaccag
     2401 aggtgatttt ctctttttat tatcgccatc ttatttgcga cgagattttg cagctcgctc
     2461 ccgcaggtgc atttaatctg cacggttcgc tgttaccaaa atatcgtggt cgcgcgccgc
     2521 tgaactgggt gctggtgaac ggtgaaacgg aaacgggcgt tacattgcac cgaatggtaa
     2581 aacgtgccga tgctggggcc attgtggcgc aactgcgcgt tgccattgcg ccagacgata
     2641 tcgctattac gctgcatcat aaattgtgcc atgccgcgcg ccagctactg gaacagacat
     2701 tacccgccat taaacacggt aatattctgg aaatcgccca gcgcgaaaac gaagccacct
     2761 gttttggtcg cagaacgccg gatgacagtt tccttgaatg gcataaaccg gcatccgtac
     2821 tgcacaacat ggtacgtgcc gttgccgatc cgtggccggg tgccttcacc tatgttggca
     2881 atcagaaatt taccgtctgg tcgtcgcgtg ttcatcctca tgccagcaaa gcacagccgg
     2941 ggagcgtgat ttctattgca ccgctgttga ttgcctgtgg cgatggcgcg ctggaaatcg
     3001 tcaccggaca ggcgggcgac ggcattacta tgcagggctc gcaattagcg cagacgctgg
     3061 gcctggtgca aggttcacgc ttgaatagcc agcctgcctg caccgcccga cgccgtaccc
     3121 gggtactcat cctcggggtg aatggcttta ttggcaacca tctgacagaa cgcctgctgc
     3181 gcgaagatca ttatgaagtt tacggtctgg atattggcag cgatgcgata agccgttttc
     3241 tgaatcatcc gcattttcac tttgttgaag gcgatatcag tattcattcc gaatggattg
     3301 agtatcatgt caaaaaatgt gatgtcgtct tgccgctggt ggcgatagcc acgccgattg
     3361 aatatacccg caacccgctg cgcgtatttg aactcgattt tgaagagaat ctgcgcatta
     3421 tccgctactg cgtgaagtac cgtaagcgaa tcatcttccc gtcaacttca gaagtttatg
     3481 ggatgtgtag cgataaatac ttcgatgagg accattctaa tttaatcgtc ggcccggtga
     3541 ataaaccacg ctggatttat tcggtgtcaa aacaattact tgatcgggtt atctgggcct
     3601 atggcgaaaa agagggttta cagttcaccc tcttccgccc gtttaactgg atgggaccac
     3661 gactggataa ccttaatgca gcgcgaattg gcagctcccg cgctattacg caactcattc
     3721 tcaatctggt agaaggttca ccgattaagc tgattgatgg cggaaaacaa aaacgctgct
     3781 ttactgatat tcgcgatggt atcgaggcgt tataccgcat tatcgaaaat gcgggaaatc
     3841 gctgcgacgg tgaaattatc aacattggca atcctgagaa cgaagcgagc attgaggaac
     3901 tgggcgagat gctgctggcg agcttcgaaa aacatccgct gcgccatcat ttcccaccgt
     3961 ttgcgggctt tcgcgttgtc gaaagtagca gctactacgg caaaggatat caggacgtag
     4021 agcatcgtaa accgagcatc cgcaatgccc accgctgcct ggactgggag ccgaaaattg
     4081 atatgcagga aaccatcgac gaaacgctgg atttcttcct gcgcaccgtt gatcttacgg
     4141 ataaaccatc atgaccaaag taggcttacg cattgatgtc gatacctttc gtggcacccg
     4201 tgaaggcgtg ccgcgtctgc tggaaatctt gagtaagcat aatattcagg ccagcatttt
     4261 tttcagcgtc ggcccggaca atatgggccg ccatctctgg cgactggtga agccacagtt
     4321 tttgtggaag atgctgcgct caaatgcggc atcgctttat ggctgggata ttttactggc
     4381 aggtacggcc tggccaggta aagagattgg tcatgccaat gccgatatca ttcgtgaagc
     4441 ggctaaacat cacgaagtcg gcctgcacgc ctgggatcac catgcctggc aagcccgtag
     4501 cggtaactgg gatcggcaaa caatgatcga cgatattgca cgcggtcttc gcactctgga
     4561 agagattatc ggtcaaccgg taacctgttc tgccgctgcg ggctggcgtg ccgaccagaa
     4621 ggtgatcgaa gcaaaagaag cgttccattt gcgctacaac agcgattgtc gtggggccat
     4681 gccgttccgt ccattgctcg aatcaggaaa ccctggcact gcgcaaattc cggtgacctt
     4741 acccacctgg gatgaagtga ttggtcggga tgtgaaagca gaagatttta acggttggtt
     4801 actcaaccgc atcctgcgag ataaaggcac gccggtttat accattcatg cagaagttga
     4861 aggctgcgct tatcagcata attttgtgga tctcctcaaa cgcgcagctc aggaaggcgt
     4921 gacattttgc cctttaagcg aactgttatc agagacgttg ccgctcggac aagttgttcg
     4981 cggaaatatt gccggacgtg aaggctggct gggttgccaa caaattgcgg gtagtcgctg
     5041 atgaaatcgg tacgttacct tatcggcctc ttcgcgttta ttgcctgcta ttacctgtta
     5101 ccgatcagca cgcgtctgct ctggcaaccc gatgaaacgc gttatgcgga aatcagtcga
     5161 gaaatgctgg catccggcga ctggattgtg ccccatctgt tagggctacg ttatttcgaa
     5221 aaacccattg ccggatactg gattaacagc attgggcaat ggctatttgg cgcgaataac
     5281 tttggtgtgc gggcaggcgt tatctttgcg accctgttaa ctgccgcgct ggtgacctgg
     5341 tttactctgc gcttatggcg cgataaacgt ctggctctac tcgccacagt aatttatctc
     5401 tcattgttta ttgtctatgc catcggcact tatgccgtgc tcgatccgtt tattgcattc
     5461 tggctggtgg cgggaatgtg cagcttctgg ctggcaatgc aggcacagac gtggaaaggc
     5521 aaaagcgcag gatttttact gctgggaatc acctgcggca tgggggtgat gaccaaaggt
     5581 tttctcgccc ttgccgtgcc ggtattaagc gtgctgccat gggtagcgac gcaaaaacgc
     5641 tggaaagatc tctttattta cggctggctg gcggttatca gttgcgtact gacggttctc
     5701 ccttggggac tggcgatagc gcagcgagag cctaacttct ggcactattt tttctgggtt
     5761 gagcatattc aacgctttgc actggatgat gcccaacata gagctccgtt ctggtactac
     5821 gtgccggtca tcattgccgg tagcctgccg tggctgggat tactccccgg tgcactgtac
     5881 acaggctgga aaaaccgcaa gcattccgca accgtctatt tgttgagctg gacgataatg
     5941 ccgctgctgt ttttctccgt cgctaaaggt aaattgccca cctatattct ttcctgcttt
     6001 gcatctctgg caatgctgat ggcgcattac gctttgctgg cagcaaaaaa taatcctctg
     6061 gcgctgcgga ttaatggctg gattaacatc gcttttggcg tcactggcat tattgccaca
     6121 tttgtggtct ccccctgggg accaatgaac acgccggtgt ggcaaacctt cgagatctat
     6181 aaagtctttt gtgcctggtc gattttttcg ctatgggcat ttttcggctg gtacacctta
     6241 acaaacgtcg aaaagacctg gtcttttgcc gcgctttgcc cgctggggct ggcgttgctg
     6301 gtaggatttt caattcctga cagagtcatg gaaggaaaac atccgcaatt ttttgtcgag
     6361 atgacacaag aatcacttca gccaagccgc tatattctta ctgatagcgt cggtgttgcc
     6421 gcaggtctgg cgtggagcct gcaacgcgat gacatcatca tgtatcgcca gacaggtgag
     6481 ttgaaatacg gccttaatta tccggatgcg aaagggagat ttgtcagcgg tgatgagttc
     6541 gcaaactggc ttaatcaaca tcgtcaggag gggattatta cactcgtgct ttcggttgac
     6601 cgcgatgaag atatcaacag tctcgccatt ccgcccgcag atgccatcga tcgtcaggag
     6661 cgtctggtgc tgattcagta tcgtcccaaa tgatctggct aacattagtc tttgccagct
     6721 tgcttagcgt tgccgggcag ttgtgtcaga aacaggcaac ctgctttgtg gcgataaaca
     6781 aacggcgcaa acatatcgtg ctgtggctgg gactggcgct ggcttgtctt ggtcttgcca
     6841 tggtgctctg gctgctggtc ttgcagaacg taccggtagg cattgcctac ccgatgttaa
     6901 gtctgaattt tgtctgggtg acgctggctg cagtaaaact gtggcacgaa ccggtatcac
     6961 cgcgtcactg gtgtggggtg gcgttcatta ttggtggcat tgtgatcctc gggagtacgg
     7021 tgtaatgggc ctgatgtggg gattattcag cgtcattatt gcctcagttg cgcaattaag
     7081 cctgggtttt gcggcgagtc atctgccgcc gatgacgcac ttatgggatt ttatcgcggc
     7141 tctgttagct tttggcctgg atgcccggat actgttactc gggctgctgg gttatctgct
     7201 ttccgttttt tgttggtata aaacgctaca taaacttgcg ctaagcaaag cctatgcctt
     7261 gctcagtatg agttatgtgc tggtatggat tgcttcaatg gtacttcccg gctgggaagg
     7321 gactttttcg ctgaaagcac tactgggagt agcctgtatt atgagcgggt tgatgctgat
     7381 tttcctgccc atgacaaaac aacgttactg agttttccct gccactttac actgccgttc
     7441 ccactcatcg gggctataac tgcttgccga gaggactttc accgtatgca gcttttcacg
     7501 attaatacaa taaagcgcat tctgcaaagg cgagagtaaa tcccccactt tcacggcgaa
     7561 gtcactcttc acttcggcga tcatttttat cgcgccgcca gcatcgcaga gcataagtac
     7621 atgtctgtta tcttgtttat tgcagcacga ttttttgacc agccattcca ttgcattatc
     7681 ctgtttgcta agagttttca catcaattta cagattaaca gataaactct ttatcaacgg
     7741 caatggaatg gcagaaatga gaagtgaaac ggagggttaa ttattgctga cgttgcaccc
     7801 actcttttag cgcctgacgt gaaattttaa taccgccgtt tttcagttca ggcggcaacg
     7861 ttaaccaacg caccggttgc tgaaaactgg ccagcttatc tttcacccat tcactaagat
     7921 caacgctctc gtggtcatac tccatcaccg ccaccggtcg atgaccaaac tccttgtcgg
     7981 caacggggac gataaacacc tgcaaaaccg caggatgtgc agcaataaca cgctcgactt
     8041 cttccggctg aataccctct ccgccactga agaatagatt gtctaaacgt ccgacaatgg
     8101 tcagcttgcc attatgcatc tcaccgcgat cgcgcgtagc gtaccagcct tcgtcattaa
     8161 ccagtgaaac cagttgcccg ttacgccagt aaccttctgc catactggca gcccgcagcc
     8221 acacttcatt attaacgatt ttcacttccc gacccggcag cggcgaacca acgtctgcca
     8281 ggccgtcggc ttctttcgca cacaccgtgg aggcaaactc ggtcagacca tagccgcaaa
     8341 agcaacgaat cccctgctcg cgcgcctgtt ccgtcaactc gaccgggata gccgcgccgc
     8401 caagtaacac cgctttcagg gaaacggaac tgcggttaac cagcaaacgc cagagttgtg
     8461 ttggcaccag tgaagcatga gtacagcctg ccagcatttg ctccaatggc tgtttatcac
     8521 gcaccgtcat ccgcgcacca gcgtataacc agcgccacat aattccctga ccggagacgt
     8581 gaaacagcgg taaagagagc aaccagtcat cgtgatcgcc aaacggaatc aacgataaca
     8641 ccccttcggc actggcaaga tgggcctgat aagtatggac agcggctttc ggcaacccgg
     8701 tagaacccga ggtcaacgtc attgagcaca gacgcgtcgg ctgccacgta gcggcatgtg
     8761 cgccttcaac ccgctgaatg tgcagcgacg ttaacgccgg aaacgtgttt tccccatccg
     8821 gcaccagcgc aaattgcagc gtcagattgg gcagcaattc ttcaagcaac ggttgcggca
     8881 gctgagggtt cacgggcaac acccgcgccc cgcattgcag taacgccagc caggcgagca
     8941 gcgtttgcgg cgtattccac gcccgcaaca tcacgccgct gccctcaaca acgccctgcg
     9001 ccgcaaagcc cgtagctaac tcatcgacgc gagcacaaag ctcgcgccag ttaagttgct
     9061 cgtcattaag acgtaaggcg atggcttctc cccgcacttg ccgccagtga cgccacggcc
     9121 agtcagagaa gatcataaca accgctccag tgcatcaact tccacgacag gcagcgtgct
     9181 acccggccag cgacgtacct gctgcgcctg catcagatcc agcgtgtcca gccctggaat
     9241 ggtgtccggc gttaaccagg cggcaatccg cgccagttgc gttaagccta agctcgattc
     9301 aatggaagaa ctgatcaccg ccgtcagccc cagcgcgtgc gccgcctgta cctgctcgcg
     9361 tactttctcc agactgcccg tgagcgtggg tttgataact accgcgcgca cgccctcttc
     9421 agccacaaag gcaaaatccg gctcgcgcaa gctttcatcc caggcaatgg caatgccggt
     9481 ttcacgggca aacgctcgcg aatcatcgcg ggttttgcac ggctcttcga gaaacgcgat
     9541 gcggtggcga taatccgggt taacatattt ggcaaactgc tggcctttca gcggtgtcca
     9601 ggcgcgattt gcgtcaagac gcaaatgcag atccggaatt gcctccagca acagattaac
     9661 caccatgccg tcgcgcacgg cttcgtacaa tcccactttg accttcgcca ctttctcgcc
     9721 tggcatatca gcaagtttga ggatcagatc gtcaggatcg ccattacaca gtggtgccgc
     9781 acggtaattg gccgcctgcg ggagcgtatc tgccagctcc gccaatgcac agcttacgcc
     9841 aaaggcaacg gaagtcatct gcggtagctc gcaatcgcct gccagccagt tatttaccca
     9901 ggcaagcagc acgctttgcg cctcttccca ggtttcctgg ctgaagcccg gcagtgggga
     9961 gatctccccc cacccttcgc gctcgccctc acgcaggcaa acgtacagcc cgtcgcgggt
    10021 ttttaaccgc ctgtcgcgca gaaccacccc cgcgtccatg gggatctgcc agcggtatac
    10081 ctgcgcgcta cgcattacgg attccgttta aatttgctga aatcaggctg acgtttctgg
    10141 ttgaaagcgt tgcgaccttc ctgaccttct tccgtcatgt agaacagcat ggtggcgttg
    10201 cccgccagct cctgcagccc cgcctgcccg tcacagtcgg cgttcagcgc agctttcagg
    10261 cagcgcagcg ccatcgggct gttttgcagc atttcgcggc accaacggac ggtttctttt
    10321 tccagatccg ccagcggtac cacggtgttg acaaggccca tatccagcgc ctgttttgcg
    10381 tcgtactgac ggcacaggaa ccagatttct cgcgcttttt tctgcccgac gatacgagcc
    10441 atgtaggaag cgccccagcc gccgtcgaag gaaccgactt tcgggccagt ctgaccgaag
    10501 atggcattat ctgctgcgat agtcaggtcg cacatcatgt gcagaacgtg accaccgccg
    10561 atggagtagc cagccaccat cgcgacaacc ggtttcggac aggtacggat ctgacgctgg
    10621 aagtccagca cgttcaagtg atgtacgccg gaatcatctt tatagccgcc gtaatcacca
    10681 cgcactttct ggtcaccacc ggagcagaac gctttatcgc ctgcgccagt cagaatgatc
    10741 acgccgatat tgtcgtcata acgcgcatct gccagtgcct ggatcatctc ttttaccgtc
    10801 agaggacgga aggcattgcg cacctgcgga cgattaatgg tgatttttgc gataccgtcg
    10861 gtggattttt cataacgaat gtcctcgaaa ccttcggagc agtcgtgcca ttcaaccggt
    10921 gcgtaaagca ttgcttcatc aggataaatc atattgtgtc ctttagtcag aaacgcaaga
    10981 tctgcgccag acttgcgatt acgccagcgg gattttcccg atgcgcgtta tgtccggcgc
    11041 gaggaatgac atggcagtcg gcagccagtt ccgccgccag ggcgcggaat ttgctgtcac
    11101 gttcaccaca taaataataa aacgcaaatg tgcgggcgct aaggttagca cgtaaatcag
    11161 gctggacggc gagagaagtc gcctccagca tggcggcaag ggttgcgcca ttattgttgc
    11221 tgcgcagcac caccagctcc cggcgttgat cgtcattgag cgaggcaaaa acaggctgtt
    11281 gataccagtc ggcaaatacc gccgttaacg gttctgtgca aaaacgctgc gcccattggc
    11341 gatcggaacg ctgacgttcc gcacgttgtt cagcattttg cagccccgga tgcccgcctt
    11401 cgacaataac cccacaaagc cccgtcagcc cctggcaagc cgccatcatc gccacccgtc
    11461 cgccaagcga gtaccccacc agccagaagt caaggatgtt gtaactaacc aaggttttac
    11521 gcagtaagtc ggtgacatca tcaaatccat cgacgctaat cgccgccgaa ccaccatgac
    11581 ctgggagatc aacatacaac cgtgagtagt cggcaaacgc ctcgcccact tcttgccatt
    11641 cgtggcaatc gccggaaaaa ccgtgcaaaa acaccagcca gggtaaacct ggttttccgt
    11701 gttttgcctg cgcgtgcagg atcataaatg gcttacctgc gccagaagtt gctggagcgt
    11761 ttgcgcacca tcggtgtcgt taaccaccat ttcaatcacc gtggtggttg gcgtgcgcca
    11821 ggcgtcggca aatgccgttt caagttcctg ccagttttgc ggacgatgat atttcagctc
    11881 gaacatcgcg gcggcgtgct caaaatggac gttttgcggc atcagataga aacgctcacg
    11941 ctcgctttgc ggcgttggca acagcgagaa aatttgcccg ccgttgttgt tcaccacaat
    12001 taataccagc ggcgcagaaa cctgacgcaa taacgccagc gcgttgagat cgtaaagtgc
    12061 ggagagatcg cccacaatcg ccagcgtcgg tttgccgctt gcccgctgaa cgcctgcggc
    12121 ggtcgaaagc agtccgtcga taccgctggc ccctcggttg ctgtacaccg ggtaacctgc
    12181 cggaagttgc gaaagcgcat caatcagacg taccaccagg ctgttaccaa caaacaattg
    12241 cccctgttca ggcagatagt cgcagatgcg atgcgccagt tgcgcttcgc caaacgcatc
    12301 acggcgggca ataaccgcct gcattgcctg ttccgccagg cgcgggattt caacgcacca
    12361 gggctggcgt ttttctgccg gatgcagctc cagccagtcg gcaatattgg caattaagcg
    12421 acgtccgcga tggtgtgccg gatcaagtcg cccttcaatg tcatcaacaa tccagtactc
    12481 ttctggttca cagcttgcct gccattgcag gagccgtttc cccgtcaggc tgcttcccag
    12541 ttgcaccaca atttgcgcct gctgcagctc gctggtcgct ttggcattgc ctaaccaaag
    12601 atcggcacac ggcagcggct gcccggtttg tgacagcaca tcgccaatca gcggccagcc
    12661 aagagtttgc gcccacaggg caactttttt gccctcttcc gcactcatgc gcccggcaac
    12721 caccacgccg cgcttttgtc gccagaagaa ccagtcgcgc tgtttttcac tttccagacg
    12781 aggcgcttca cgcagccacg gtttgtcgtc ctgccaccag tcacccagac gctgttgcca
    12841 gctaagcccg gtatcgtcca tttcgccata cagcggttca gcaaacgggc agttgatatg
    12901 gactcccccc gcatgaagcg taccgagagc gtggtcgatg gtagaaacca gccaacgtgc
    12961 ggggatatcc tgggtcgggc gcggcaatga aatactgtgc gtggggtgag aggcgaacat
    13021 tcccggctgg cgaattgcct gattcgcgcc gcagtcaatt agctccggcg ggcgatcggc
    13081 ggttaagaga atcagttttt ctccggttaa cccggcttca atcagtgccg gatagagatt
    13141 tgccaccgcc gtgccggagg tcacaatcac cgccaccggc tgcttgctga ctttcgccag
    13201 ccccagcgcc agatgcccca acccacgctc atcgaaatgg gtgtggtgaa tgaatgcgga
    13261 attctccgcc gccgctaacg ttaacggtgt agaacgcgag cctggggcga tacagatgtg
    13321 tctgacgccg tgacgcgtta atgcttccag aatgaccgcc gcccagcgtc ggttaaatgc
    13381 gcttactgac atgagtttgt ccggtatcaa tattgcggct aagtataagg agctacaaaa
    13441 atagattatt gatatgaatc ggtaatgatg cgactcatta ctattccatt tgtaataaag
    13501 tacgcagccc tgccgctttg ttgtcgattt cctgccactc ttgctcgggg tcggaaccac
    13561 ggacaatgcc cgcgccagca tataatcgca cgacattgcc gctaattttt gctgagcgca
    13621 gggaaacgca gaattcgctt tgttgtaatg agagatagcc cgcagaaccg gcgtaccatt
    13681 ctcgggtgaa cggttcgtga cgggcgataa actgtcgcgc cagatcgcgc ggtaagccag
    13741 caactgctgc cgttggctgc aactgatgta aacagatcac atcatccgct ttgttgagtg
    13801 aagtccagat acagcggcga agatgctgca ctttacgcag acgcagtacc tgcggcggta
    13861 aaacatccag cgtctgggta tcggcctgta atcgttggca gatatcttcc accaccagca
    13921 tgttctcgcg ccggttttta tcatccgcca tcagccactc tcctaactgc tgcgcctgct
    13981 tatcatcagg attatttgct actgttcccg ccagcgcttc agtacgcagc gctttgtcac
    14041 gccgccgcca taaccgttcc ggtgaagagc caagaaaagc attttcgcca tcaaaggcca
    14101 tgtaaaaatg gtagcaattc agattcagtc gacgactggc agccatcatc gccgccgcgt
    14161 tgaccggact tgcgaaatgc aggtcagttg cccgagcgag caccactttg tcgagctcac
    14221 cttcggcgat ggttttcgtt gccagttcga ttaattgcgt ccagcccgtt ttgtccggcc
    14281 agtgttgttc tcgcgtggtg gttaaatgta acccaggcaa gggcttgata ctcaccagtg
    14341 tggcgataaa ttcttttgcc tgaatcgcat cgtgctgaag ggagctttcg ctgaatagcg
    14401 tcagccgcag cgtggcttta ccgccacagc gtcgccattc caggcggggt aaaagtaaat
    14461 tgccctgcga cgggtcaaaa gcattcagcc cccaaatgcg taagtcggcg tgttccgggt
    14521 gctggcgaag aaaacgttgt gcctggtcca acgacgtaaa acgggtaatc gcgcccagga
    14581 cggcagcttc ttcatcacca ttacgttgtt gccagtagaa ttgcgggtac gtttgctgac
    14641 tggccagcca gctcaaggca tcaaaagcgt ctttgagagg gaaaggaata tcgataaccc
    14701 gaatgccggg tgtcgccgga atctcttgcg acaaatggcg cagtagattt tccagcgccg
    14761 tagtaagtga ttgcacgcgg acctctccct gttaaaagcc tcacattata cggggtacta
    14821 caaaaaaaat gcagtacccc ggtgtaggga ggttttaacg gcgtgccagc aacagtccta
    14881 gtaccagccc aacggccgcg cccacaccaa ttccttgcca gggtttttcg tggacgtagt
    14941 catcagcacg ataaactgcc tgcttcgccc gatagtaata actgtctgaa gcctggctaa
    15001 cccgtttttt cacatcatcc agcgcttttt ctgcacgcgc tttcagctca acatatttct
    15061 gatcggcggt atcgccagag gagcggagca cctcttccag tgtttcacta agcagcgtca
    15121 ggtcgtcatc gatacgtgta tcaccaaact gattagacat tctcgttctc catttgcgta
    15181 aaacctgtca ctaactatag acaagggttg ttgattacgc ctgaattacc tcgcgtgcca
    15241 tcccaatgtg tgggatacca tcttcttcat agacctccgt cacagggata aaaccgaaac
    15301 tctggtagaa gttttgcaga tgcgcctgcg ccccgaggta aacaggctta tcaggccagt
    15361 gatgcgtaca tgtttcgagt gttttactca tcagctgctg ccccactttt tcgccacgca
    15421 acgcttcact gacaatcacc cgacctataa cgaccggctc aagatcatca tcacttttca
    15481 gaatcctcgc atatgccacc agttcatcat ttttccaacc gagaatatgg cgattatccc
    15541 ccgtcaaatc atcaccatcg atatcctgat aagggcaatt ctgctcgaca acaaacaccg
    15601 cgcaacgcag ttgtaataag gcataaagct gggaaaccga taattcagag tgatgcagat
    15661 cttgccattc aatcataatg ctctcctttt agatgtatgc cttatactag ccggaatatt
    15721 ttttgaaacg tgatgaactc atggaattaa tttttttagg tacttcggcc ggtgtgccaa
    15781 cccgcacgcg caatgtcacg gcaatattgc ttaacctgca acatccgacc cagagcggtc
    15841 tttggttgtt tgactgcggt gaaggcaccc agcatcagct actgcatacc gcctttaacc
    15901 ctggaaaact ggacaagatt tttatcagtc accttcatgg cgatcatctt tttggtttac
    15961 ccggcttgct gtgcagtcgt tctatgtcag gcattatcca acccttaacg atttatggtc
    16021 cgcaaggtat ccgtgaattt gtggaaaccg cgctgcggat tagcggctca tggaccgatt
    16081 atccgctgga aattgtcgaa attggcgctg gcgaaattct cgatgatggc ctgcgcaaag
    16141 taaccgctta tccgctggaa cacccactgg aatgttatgg ctatcgtatt gaagaacatg
    16201 ataaaccggg tgcattaaat gcccaggcat taaaagctgc tggcgtgccg cctggcccac
    16261 tgtttcagga attaaaagcg ggcaaaacaa tcacgctgga agatggaagg cagattaacg
    16321 gcgcagatta cttagctgct ccagtgccag gtaaagcgct cgctattttc ggcgataccg
    16381 gcccctgcga tgccgcactt gacctggcta aaggtgtcga tgtcatggtg cacgaagcga
    16441 cgctggatat aaccatggaa gccaaagcca atagtcgcgg ccatagctct acacgccagg
    16501 ctgcgacact agcccgtgag gctggagtcg gtaagctaat cattacccac gtcagctcgc
    16561 gctatgatga caaaggttgt cagcacctgt tacgtgaatg caggtcaatt ttcccggcga
    16621 ctgaactagc gaatgatttc accgtgttta acgtttaact ctgcttatta ttaaaaacag
    16681 ggcgaaactt gccctgttat cgcaacccgc gctacatata tccacccaca cgggcatatt
    16741 gagcgatttt tgatatttat tcatctcctt acgcactata ttcagagcaa taagttgcct
    16801 ctttttatct tcattcagca caacctttgc aactaaaagg agcccatgat ggttacagtt
    16861 gtcagcaatt attgtcaatt atctcaaacc caactcagtc agacatttgc agaaaaattt
    16921 actgtgaccg aggaattact gcagtcttta aaaaaaacag cgttatccgg agatgaagaa
    16981 agtattgagt tactgcataa tattgcgtta ggttatgatg aatttggaaa aaaagctgaa
    17041 gatattcttt accatattgt tagaaaccca acaaatgaga ccctatcgat tatccgactt
    17101 attaaaaatg cctgtttaaa attatataat ttggcacata ccgcaaccaa ctcccacctt
    17161 aaaccaactg gtccggataa ttcagatgtt ctcctgttca aaaagctatt ccccccttcg
    17221 aaattaatga caattatcgg tgatgaaatt cctcttatat ctgaaaaaca gtcgctttca
    17281 aaggtgcttt taaatgatga gaataatgaa ctgagtgatg atacaaactt ctgggataaa
    17341 aatcgtcaat taaccacaga tgaaatagct tgctatcttc agaagatcgc cgctaatgca
    17401 aaaaatactc aagtcaatta tcctactggt ctctacgtcc cctactccac cagaactcac
    17461 ctggaagacg ctctcaatga aaatattaag agcgatccat catggccgaa tgaagtccag
    17521 ttattccaca taaatactgg cggacactgg atattagttt cgctac
//
LOCUS       DBANUU010000091        16713 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000091 DBANUU010000000
VERSION     DBANUU010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.091"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            288..440
                     /gene="mokC"
                     /locus_tag="ACIE68_003138"
     CDS             288..440
                     /gene="mokC"
                     /locus_tag="ACIE68_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFX5909413.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(544..1674)
                     /gene="dnaJ"
                     /locus_tag="ACIE68_003139"
     CDS             complement(544..1674)
                     /gene="dnaJ"
                     /locus_tag="ACIE68_003139"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFX5909414.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1763..3679)
                     /gene="dnaK"
                     /locus_tag="ACIE68_003140"
     CDS             complement(1763..3679)
                     /gene="dnaK"
                     /locus_tag="ACIE68_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFX5909415.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4056..4460
                     /gene="yaaI"
                     /locus_tag="ACIE68_003141"
     CDS             4056..4460
                     /gene="yaaI"
                     /locus_tag="ACIE68_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFX5909416.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGAIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4486..5199
                     /gene="msyB"
                     /locus_tag="ACIE68_003142"
     CDS             4486..5199
                     /gene="msyB"
                     /locus_tag="ACIE68_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFX5909417.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLEHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5348..5914
                     /gene="satP"
                     /locus_tag="ACIE68_003143"
     CDS             5348..5914
                     /gene="satP"
                     /locus_tag="ACIE68_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFX5909418.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5949..6536)
                     /gene="mog"
                     /locus_tag="ACIE68_003144"
     CDS             complement(5949..6536)
                     /gene="mog"
                     /locus_tag="ACIE68_003144"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFX5909419.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6651..7604)
                     /gene="tal"
                     /locus_tag="ACIE68_003145"
     CDS             complement(6651..7604)
                     /gene="tal"
                     /locus_tag="ACIE68_003145"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFX5909420.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7883..9313
                     /gene="yaaJ"
                     /locus_tag="ACIE68_003146"
     CDS             7883..9313
                     /gene="yaaJ"
                     /locus_tag="ACIE68_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFX5909421.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFTLLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            9383..10159
                     /gene="yaaA"
                     /locus_tag="ACIE68_003147"
     CDS             9383..10159
                     /gene="yaaA"
                     /locus_tag="ACIE68_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFX5909422.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(10312..10608)
                     /gene="yaaX"
                     /locus_tag="ACIE68_003148"
     CDS             complement(10312..10608)
                     /gene="yaaX"
                     /locus_tag="ACIE68_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFX5909423.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10822..12108)
                     /gene="thrC"
                     /locus_tag="ACIE68_003149"
     CDS             complement(10822..12108)
                     /gene="thrC"
                     /locus_tag="ACIE68_003149"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFX5909424.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFSAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQTLVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12109..13041)
                     /gene="thrB"
                     /locus_tag="ACIE68_003150"
     CDS             complement(12109..13041)
                     /gene="thrB"
                     /locus_tag="ACIE68_003150"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFX5909425.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQEQGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13043..15505)
                     /gene="thrA"
                     /locus_tag="ACIE68_003151"
     CDS             complement(13043..15505)
                     /gene="thrA"
                     /locus_tag="ACIE68_003151"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFX5909426.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15531..15650)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15586..15651)
                     /gene="thrL"
                     /locus_tag="ACIE68_003152"
     CDS             complement(15586..15651)
                     /gene="thrL"
                     /locus_tag="ACIE68_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFX5909427.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15865..16551)
                     /gene="yjtD"
                     /locus_tag="ACIE68_003153"
     CDS             complement(15865..16551)
                     /gene="yjtD"
                     /locus_tag="ACIE68_003153"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFX5909428.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         3887 a         4435 c         4140 g         4251 t
ORIGIN      
        1 gtcaataatt gcgatgaaga gataaattaa agttaaataa ttaaatcatt aaaaaattac
       61 gccacaaaaa tgcgaggcgt cttcaggatt agaatcgata aaacaaaatg ggttaaagag
      121 agcgatataa tagcggcggg tgcttgaggc tgtctgtctc aggcattagc tgaacggcag
      181 atagagaaaa gccccgagtg atattttacc atcaacccga ggcctcctat atgctgaaca
      241 catgtagagt gcctcttact gaccgtaagg tcaaggagaa gagagcaatg aagcagcata
      301 aggcgatgat tgtcgccctg atcgtcatct gtatcaccgc cgtagtggcg gcgctggtaa
      361 cgagaaaaga cctctgtgag gttcacatcc gaactggcca gacggaggtt gctgttttca
      421 cggcttacga atccgagtaa gagcaacggc ggggagtgat ccccgccact ttacaggtgc
      481 tcgcatatct tcaacgcacc ctatttttac ccaggcctgc ccacgggcag gcttttgggg
      541 aggttagcgg gtcaggtcgt caaaaaactt cttcacacca tcaaagaagc tctttgagcg
      601 cgggctgttg tgctcgccgg ttgggccacc gaagctttct tgcagctctt gcagcagctg
      661 tttctgcttt tcgttcaggc ctaccggtgt ttcgacgaca acgcggcaca gcaaatcacc
      721 ctgtgcgcca ccgcggacag acttgacgcc tttaccgcgc atacggaata gcttaccggt
      781 ctgggtttcg ccaggcactt tcagtttgac gcgaccatca agggtcggta cttcgatttc
      841 gccacccagc gccgccatag cgaagttgat cgggacttcg caatacaggt tgttgccttc
      901 acgctcgaaa atcgggtgct gtttaacctg aacctgaacg tacagatcgc ctgccggtgc
      961 gccatgctcg cccgcttcac cttcgcccgc aagacggatg cggtctccag tgtccacccc
     1021 tgccgggatt ttaacggaca gcgttttgct acgctcaaca cgaccatgac catgacattt
     1081 gttgcacgga tctttgatca gcgtaccgcg gccctgacag tgtggacagg tctgctgtac
     1141 agcgaagaat ccctggcgca tctgcacctg accagaacca tgacaggtcg gacaggtctg
     1201 cggctgtgta cctggttttg caccgctacc gtggcaaacg tcacactctt ccagagtcgg
     1261 aatgcggatc tctttggtca cgccacgtac agcttcttcg agggtgagct ccatgttata
     1321 gcgtaaatca gcaccgcgcg ccgcacgttg acgaccacgt ccgccgccaa aaatatcgcc
     1381 gaaaacgtca ccaaaaatat cgctgaagtc tgcgccgccg ccaaaaccgc cgccgcccat
     1441 gccaccttgc tcaaacgcag catgaccata ctgatcgtat gccgcacgtt tttgcgagtc
     1501 ggtcagaact tcataagctt ccttgatctc tttaaatttc gcctcggcct ctttgtcacc
     1561 ctggttacgg tccgggtggt atttcatggc caggcgtttg taggcctttc tgatttcacg
     1621 ctcttccgct gttttggaaa cgcctaaaat ctcgtaataa tcttgcttag ccatcttttt
     1681 taaattgccc ctagatgaat gcacgggcgg agaggaaatt ccccttcgcc cgtgtcagta
     1741 taattacccg tttatagggc gattattttt tgtctttgac ttcttcaaat tcagcgtcga
     1801 caacatcgtc atctttcgcg ttgtttgcag aagcatcagc accggcagtc tgctgctggg
     1861 catgttgctg ctgggcgatt tccatcagtt tctgggaaac ctgtgccagt tcctgcattt
     1921 tcgcttcgat agcggctttg tcttcacctt tcagagcagt ttccagtgca gtcagcgcag
     1981 actcgatagc agttttgtcg tcagccggca gtttgtcgcc tgcttcttca acctgcttac
     2041 gggtgctgtg cagcagatgg tcgccctggt tgcgagtctg taccagctct tcaaacttac
     2101 ggtcagcttc ggcgttagct tctgcgtcgc gtaccatttt ctggatttca tcttcgttca
     2161 gaccagaaga agccttgatg gtgatcttct gctctttacc gctgttttta tctttcgcgg
     2221 aaacgtgcag gataccgtca gcatcgatat cgaaggtaac ttcgatctgc ggcatgccgc
     2281 gcggtgccgg gttgatacca tctaggttga actgacccag agatttgtta tcagccgcac
     2341 gtttacgttc accctgcagc acatggatgg ttaccgcaga ctggttgtct tcagcggtag
     2401 agaacacctg gctgtgcttg gtcgggatag tggtgttttt cgcgatcagc gtcgtcatca
     2461 caccgcccat ggtttcgata cccagagaca gcggggtaac gtccagcagc agtacgtctt
     2521 ttacgtcacc agtcaaaaca ccaccctgaa cagcagcacc gattgctaca gcttcgtccg
     2581 ggttaacgtc tttacgcggc tctttaccaa agaactcagc aactttcttc tgaaccattg
     2641 gcatacgagt ctgaccacca acgaggataa cgtcgtcgat atcagatacg gacaggccag
     2701 cgtcctgcag tgcaactttc agcggctcaa tggaacggtt taccagatct tcaaccaggc
     2761 tttccagttt cgcacgagtc actttgatgt tcatgtgttt cggaccggtc gcgtctgcag
     2821 tgatgtatgg caggttaacg tcggtctgct gagcggaaga cagttcgatt ttcgcttttt
     2881 ctgccgcttc tttcaggcgc tgcattgcca gcggatcgtt gcgcaggtca atgccctgat
     2941 ctttcttgaa ttcttcaacc agatagttga tcagacggct gtcgaagtct tcacccccca
     3001 ggtgggtatc accgttggtt gccagaactt cgaaggtttt ttcgccgtca acttcgtcga
     3061 tttcgataat agaaatatcg aaagtaccac cacccaggtc ataaaccgcg atagtacggt
     3121 tgccagtgcc tttgtccaga ccgtaagcca gcgcagctgc ggtcggttcg ttgatgatac
     3181 gttttacttc cagaccagcg atacggcctg cgtctttggt tgcctgacgc tgagcatcgt
     3241 taaagtatgc cggtacggtg ataacagctt cagttaccgg ttcacccagg taatcttcag
     3301 cggttttctt catttttttc agcacttcag cagaaatctg cggcggtgcc attttctggc
     3361 ctttaacttc gacccatgcg tcgccgttat cagcagcaat aattttgaac ggcatgatgg
     3421 aaacatcacg ctgtacttct tcgtcctgga agcggcggcc aatcaggcgt ttaatcgcaa
     3481 acagggtgtt ttgcgggttc gtcactgcct gacgtttagc cggctgacca accagagttt
     3541 caccatcctg ggtataggca atgatagaag gcgtggtgcg atcgccttcg gcgttctcca
     3601 gcacgcgagg agtggtgcca tccataatcg ctacacaaga gttagtagta cccaggtcga
     3661 taccaattat tttacccatc taaacgtctc cactatatat tcggtcatca tgtggttgtg
     3721 agtctgtaat aggggcgaaa cgtctggttt caactgccca atttcatttt tttgcagact
     3781 cactcactgc ggttgactac taaatggggt cgtaacccac gtcatcaagg gggagatgca
     3841 aaaatttttt gtgcttttgc gcagaaattt cgcgatatgg caggacaaga cgtaaaattg
     3901 acagcaattt tattcatata aagaatgaat cgcagccaag gcattcatca aaaaattgca
     3961 ataaaaagaa aagattacgt gcctgaatct tctctttatc agcagtaaac tagtgggtat
     4021 tcatccccct acctcttccc actaagagaa tccttatgaa atccgttttt acgatttccg
     4081 ccagcctggc gattagcctg atgctgtgct gcacggcgca ggcaaacgac cataaactcc
     4141 tcggcgccat tgcaatgccg cgtaacgaaa ccaacgatct ggcgctgaaa cttcctgttt
     4201 gtcgcattgt gaaacgcata caactctctg ccgaccatgg cgatttacag ttaagcggtg
     4261 catcggttta tttcaaagcc gcccgtagcg ccagtcagag cctgaatatt ccttcagaaa
     4321 taaaagaagg gcaaaccact gactggatca acattaacag cgataacgac aataaacgct
     4381 gcgtctcaaa aatcaccttt tcgggtcata cggtgaactc atcggatatg gccacgctga
     4441 aaattatcgg cgacgactaa cgccagaata tagaagccac aaaaaatgaa tgttaattac
     4501 ctgaatgatt cagatctgga ttttcttcag cattgtagtg aggaacagtt ggcaaatttc
     4561 gcccgattgc tcacccataa tgaaaaaggc aaaactcgcc tctccagcgt cctgatgcgc
     4621 aacgaactgt ttaaatcgat ggaagggcat cccgagcaac atcgccgcaa ctggcagctg
     4681 attgccggag aattacagca ttttggtggc gatagtatcg ccaacaaact gcgcggacac
     4741 ggtaaattgt atcgggccat tttgctcgat gtttcaaagc gattgaagct gaaagccgac
     4801 aaagagatgt ctacgtttga aattgagcaa cagttactgg aacaatttct gcgtaatacc
     4861 tggaagaaaa tggacgagga acataagcag gagtttctgc acgcggtcga tgccagggtg
     4921 aatgagctgg aagagctgct gccgctgctg atgaaagaca aattattggc aaaaggtgtg
     4981 tcgcatttgc tttccagcca actgacccgc attttacgca cccacgcagc aatgagcgta
     5041 cttgagcatg gtttgctgcg cggcgcgggg ctgggaggcc ctgtaggtgc ggcactaaat
     5101 ggggttaaag cggtcagcgg cagcgcctat cgcgtgacga ttccagccgt actgcaaatc
     5161 gcctgcctgc gccggatggt tagcgccact caggtctgat accggaaacc ggggaaaatt
     5221 ttcattttcc cctcaaaaga tcgtagacac tgccccactg gctgattatt atgccgcgcc
     5281 ctgaaaacac tacagttatt cagggaaatt atttcaccat tcattcgatg atgatttttg
     5341 aggaattatg ggcaacacta agttggctaa tccggcaccg ctgggcctga tgggcttcgg
     5401 catgaccacc attctgctta acctgcacaa cgtgggttat ttcgctctgg acggtattat
     5461 tcttgccatg ggcattttct acggcggcat cgcgcaaatt tttgctggtc tgctggagta
     5521 caaaaaaggc aacactttcg gtttaaccgc attcacctct tacggttctt tctggctgac
     5581 gctggttgcg attctgctga tgccgaaact gggtctgacc gatgcgccaa atgcacagtt
     5641 ccttggtgtc tacctgggtc tgtggggcgt atttacgctg tttatgttct tcggcacgct
     5701 gaaaggcgca cgcgttctgc aattcgtttt ctttagcctg accgtgctgt ttgccctgct
     5761 ggcgatcggt aacattgccg gtaacgccgc aatcatccac tttgccggct ggattgggct
     5821 gatctgcggt gccagcgcaa tctatctggc gatgggtgaa gtactgaacg agcagtttgg
     5881 tcgcaccgtt ctgccgattg gtgaatccca ctaattgttt tgagatcccc ccgctcgggg
     5941 ggattttttt attcgctaac gtcgcgtctt gcactcttcg gtctgaatgc tgcaaccact
     6001 tccggtgccg tttcaacgta tggcccttcc agcaactgaa tgcagtacgg tacgctggca
     6061 aaaataccgt gtaccacaac gttaccctca gcgtccttca caccttccag cgtctcttta
     6121 atagacttcg gctgaccggg taagttaagg atcagcgcct gtttgcgaat cacgcccacc
     6181 tgacgcgaaa ggatcgcagt tggtacaaaa tgcaggctga tctggcgcat ctgttcacca
     6241 aagccaggca tctcgcggtc cgctactgcc agcgtcgcat cgggcgttac gtcacgacgc
     6301 gccgggccag ttccgcccgt ggtgagcacc agatggcaac tcatttcatc caccagctca
     6361 cacaacgttt gctcgatgat cgcctgctca tcggggatta agcgggtttc cagttcaaac
     6421 ggcgtggtta gcgccgatgt cagccattct tccagcgcag ggatgccttt atcctgataa
     6481 acgccgctgg atgcgcgatc agagatggaa actaagccaa tacgtaaagt attcatcaag
     6541 atttttccgt ttaaacagtc tcgttaaaca gaatgataca ctgcgaaggg agtgacagac
     6601 aggcttcaga agaggtagcg tgaccgactt cccggtcacg ctaagaatga ttacagcaga
     6661 tcgccgatca ttttttccag tttttcctgg tcaatagcaa acttacggat accttccgcc
     6721 agtttatcta ctgccattgg atcctggttg tgctgccaca ggaactcgga ctcagtgata
     6781 cgcgccggac gcgctttcac ttcgccggtg taagacagtt tacgttcgat agccccttcg
     6841 ctctccgcca gctctttcag cagtgccggt gcgatggtca gacggtcgca gcctgccagt
     6901 tccagaattt cgccgatgtt acggaagctt gcgcccataa ccacggtttc ataaccgtgc
     6961 tctttgtagt actggtagat ttcagataca gaaaccacgc ccggatcttc tgccggagcg
     7021 tactctttct tatcggtatt cgctttgtac cagtcaagaa tacggccaac aaacggcgag
     7081 atcaggaaca cgcccgcttc cgcacaagca cgagcctgag cgaaggagaa cagcagggtc
     7141 aggttacagt tgatgccttc tttttccagc tgttctgcag cacggatacc ctgccaggta
     7201 gaagccagtt tgatcagaat acgatcgttg ctaataccag catcgttgta gagtttgatc
     7261 aggcgttttg ctttcgcaat tgacgcttcg gtgtcatagg aaagacgcgc atcaacttca
     7321 gttgagatac ggcccggaac cagtttcagg atttccagac caatatttac tgccagtttg
     7381 tcggtcgcgt ccacgatctg ctgcgcgcga tcgttgctct gctgtttcgc ccaggcgaca
     7441 gcatcatcaa tcaacttacg gtattccgga atctgcgctg cgttaagaat gagagaaggg
     7501 ttggttgtgg catcctgcgg ttgatacagc ttcattgccg cgatgtcccc agtgtcggcc
     7561 actacggtgg tgtactgacg aagggaggtc aatttgtccg tcatgatagt atttctcttt
     7621 aaacagcttg ttagggggat gtaaccggtc tgccctgatg atatcacgac gcaacgtcag
     7681 cgcaaccgcc gacaagacgg ttatcgccag gcgagactgt ttcggatttc tgacactgcg
     7741 ttgatatcgc ccgccatttt tatacaaaac ctcatgtatg ctacgcagaa gttatcaagt
     7801 acctcgtagc gtatatactt cttaaacaat tggtaacgtt tacacaggaa agtcatcgcg
     7861 accggcaata agagggatat gcatgccaga ttttttctcc ttcattaaca gcgtcctttg
     7921 gggatcggta atgatttacc tgctcttcgg cgcaggttgt tggttcactt ttcgcaccgg
     7981 atttgtgcag tttcgctaca tccgccagtt tggcaaaagt cttaaaaata gcattcatcc
     8041 acagccaggc ggtttaacct catttcagtc attgtgtacc agtcttgcgg cgcgcgtggg
     8101 tagcggcaac ctggccggcg ttgcgctggc tattaccgcc ggtggacctg gagccgtctt
     8161 ttggatgtgg gttgccgcgt ttatcggcat ggcgacctcg tttgccgaat gttctctggc
     8221 acaactttat aaagaacgtg acgtcaatgg gcagtttcgt ggcggaccgg catggtatat
     8281 ggcgcgcggg ctggggatgc gctggatggg cgttctgttc gccgtctttt tgctcatcgc
     8341 ctatggcata attttcagcg gagttcaggc gaacgccgtt gcccgcgccc tgagtttttc
     8401 ttttgatttt cccccgctgg tgacaggcat tattctcgct gtctttactc tgctggcaat
     8461 cactcgcggt cttcatggcg tcgcccggct catgcagggg tttgtcccgt tgatggcgat
     8521 aatctgggtg ctgaccagcc tggtgatttg cgtaatgaat atcgggcaac ttccccacgt
     8581 catttggtct atttttgaga gtgcttttgg ctggcaggaa gcggcaggcg gcgcggcggg
     8641 atatacctta agccaggcga ttactaacgg ttttcagcgc agtatgtttt ccaatgaggc
     8701 gggaatgggt tcgacgccaa acgcggcagc ggcagcggcg tcctggcctc cgcatccggc
     8761 agcgcaaggg attgtccaga tgattggcat ttttatcgac accctggtca tctgtacggc
     8821 aagcgccatg ctgatattac tggcgggtaa cggcacaacc tacatgccgc tggaaggtat
     8881 tcagcttatc cagaaggcga tgcgggtgct aatgggttcc tggggtgctg agtttgttac
     8941 ccttgtggtt attctgtttg ccttcagctc catcgttgcc aactacattt atgccgaaaa
     9001 caatctcttc tttttacgcc tgaacaaccc taaagcgatc tggtgtttgc ggatctgcac
     9061 cttcgcaacg gtcatcggcg gcaccttgct aagccttccg ctgatgtggc aactggcaga
     9121 tatcataatg gcctgcatgg ctattaccaa tttgaccgcc attttactgc tctcgcctgt
     9181 ggttcatacc attgccagtg attatctacg ccagcgtaaa ctcggcgtgc gcccggtgtt
     9241 tgatccattg cgttatccgg atatcggtcg ccagctttct ccggacgcgt gggatgatgt
     9301 ttcgcaggag taatcacaac tatcgatcaa ctcattctca ttttttgcta aagtcggcat
     9361 aaatttcctg caaggactgg atatgctgat tcttatttca cctgcgaaaa cgcttgatta
     9421 ccaaagcccg ttgaccacca cgcgctatac gctgccggag ctgttagaca attcccagca
     9481 gttgatccat gaggcgcgga aactgacgcc tccgcagatt agcacgctga tgcgcatcag
     9541 cgacaaactg gcgggtatca acgccgctcg ctttcatgac tggcagccag atttcacgcc
     9601 ggcgaatgcc cgccaggcga ttctggcgtt taaaggtgat gtctacaccg gcttgcaggc
     9661 cgaaaccttc agcgaagacg atttcgattt tgcccaacag catttgcgaa tgctttccgg
     9721 cttgtatggc gtactccgcc cgctcgattt aatgcagcct tatcgtctgg aaatggggat
     9781 ccgtcttgag aatgcccgag ggaaagatct gtatcaattc tggggagata tcatcaccaa
     9841 caagctgaac gaggcgctcg cagcacaagg cgataatgtg gtgattaatc tggcgtcaga
     9901 tgagtatttt aaatcggtga agccgaagaa attgaatgcc gagattatca agccggtgtt
     9961 cctcgatgag aagaacggca aatttaagat catcagcttc tacgctaaga aagcacgcgg
    10021 tctgatgagt cgtttcatta ttgaaaatcg gctgaccaaa ccagagcaac tgactggttt
    10081 taatagcgaa ggttacttct ttgatgaaga ttcctccagc aatggcgaac tggtgtttaa
    10141 acgctacgag cagcgttaag tttgcagtca atgccggatg cggcgtgaac gccttatcct
    10201 gcctacaaaa gcatgcagat tcaatatatt gcagaattaa cgtaggcctg ataagacgcg
    10261 ccagcgtcgc atcaggcgct gaatgccgga ttgttacccg gcatttgtca tttagcggtg
    10321 atgtttgcct ggaccatgac cgccgtgatg atcatgagga gctttcttat ggtggcgcgg
    10381 cggtggcggc ggtccgtgtg ggtgccagcg attgcctcgc cattcataat gttgtttcca
    10441 ccagccgtgg tcgcgccagt gacctccatc ccagtaatag ccacgattat cacgatcgcc
    10501 tatctgtaat tttactgacg ggactaacgt aatttccgca gcctgtgctg ccatgggagc
    10561 gaccagaacc agggaaagtg cgagtacgat agattgcatc tttttcacag gttactcctt
    10621 ttatcattgc ccgttatggg tcgatatccg gagattactc gatgagaacg gcggttgtta
    10681 ttctccgcaa tcctaatctc taagttaccg catttattga gaatttctcc tttttccctg
    10741 tcatttttct ccataatttc ttcataaaaa agccgggctg cataaaagca aacccggcct
    10801 gattgagata atgaatagat tttactgatg attcatcatc aatttacgca acgcagcaaa
    10861 atcggcgggc agattatgtg aaagcaaggg taaatcagca cgttctgcca gctcttttgg
    10921 cagatccaac gtttcaccga gaatcgcttc cacgctctct ttaaatttcg ccggatgcgc
    10981 ggtgccgagg aacaagccat attcgcctgg attcaactga tcacgcagcg cacgataagc
    11041 tacggcagcg tgcggctccg aagtgtagcc cagttctttt aactcacgca ttgtctgttg
    11101 cgtggtttca tcatccacgg ctgcataacc cagctctttc agttgccaga ttttgcggcg
    11161 gaacaactct tccacacgcg gccagttgtt cggctgactc acgtccatcg cgttggataa
    11221 cgtcgcctga gtcgctttgg gtgaccactg accgtcgtgc aggaaacgtg gcacggtatc
    11281 gttcacgttg gtcgcagcaa taaaacgttt caccggcaga ccgagtgact tcgccagcag
    11341 acccgccgtc aaatcgccga agtttccgct tggcaccgag acaaccagct ggttgcgcgc
    11401 ctcctgcggc agctgcgcaa cagcttcaaa gtagtagcaa atctgcgcca gcaaacggct
    11461 gatgttaatc gagttagccg agtttaaccc cagcgccact ttcagttctt catcatcaaa
    11521 cgcctgcttc accagcgtct gacaggcatc gaaatcgccg tcgatggcaa cagtttcgat
    11581 attgccgccc aatgtacaga acagtttttc ttgcagtgga ctgattttgc ctcgtggata
    11641 gaggataacc actttcacat tcggtaaacc gtagaaagca tgagccactg ccgctccggt
    11701 atcaccggag gtcgcggtca gaatggtcac tggcttatca cccgcaatat gggtcagcat
    11761 ttgtgccata aagcgaccgc cgaaatcttt aaatgccagc gttggcccgt ggaacaattc
    11821 cagacaaccg acatcgcttt caacattggc gaccggagcc gagaaggcaa acgccgcgcg
    11881 cacgcgctct tccaggattt cctgtgggat ttcatcacca ataaacgccg agaggatctt
    11941 cgcactgcgg gtgacaaaat ccagcttcag catctcatca atttcagtca ggctgaattc
    12001 cggcaggtcg tgcggaaaaa acagcccctg atttttgccc aacccctggg ttacggcttg
    12061 cgcaaagctg acctgctcgt tgtgatcttt cagattgtag agtttcattt agttttccag
    12121 tactcgtgcg cccgccgtat ccagccggca aatatgaaca aaaccttcct gattttgcag
    12181 gtagttctta cccaaccagt cggcaacgcg ctgggcggta tccggcttgt cacatagagc
    12241 gaacaaggtc gggccggagc cggagatacc gctcgctacc gcgccgattt ccgcgaccgc
    12301 ctgccgcgcc tgccggaaac caggcagtaa ccgttcacgg tagggttcag cgataacatc
    12361 tttcatcagc ttcgcggcaa gctcaggctg acgggaatag caggcgtgaa tgaagcctgc
    12421 cagatgtcgc ccgtgcgcaa tgcaatcctg gcggcgatac tgcgccggta aaatagccct
    12481 ggcttctgcc gtcgagactt taatccccgg atacgccagc acccacagcc actcatcaaa
    12541 ccctggcact tgctggctga tgatgtcgtt ttcttcgatc atcaactgca taccaccaag
    12601 aaaacacggt gccacgttgt cgtaatgaat gctgccggag atacgtcctt ccagctcgcc
    12661 catcaaagcc agcaaacgag tgtcattaag cggcttgccg cagtgttcat tcatcgccat
    12721 cagcgccgcg accaccgaac aggcgctgga gcctaagccc gagccgatcg gcatattctt
    12781 ttccagagtc atcgccactg gaatttgctt gccctgctcc tggcaaaaac gctcccagca
    12841 ctgataaacg atattttccc gtggttctga cggcagctta tcggcaaagc gtccgaggtt
    12901 gttgagactg aatgtctctg ccgcctcaac cgtgactaca tctccgagca atgcaccatc
    12961 aacgggtgtc accgccgccc cgagcacatc aaacccgacg ctcatattgg cactggaagc
    13021 cggggcataa actttaacca tgtcagactc ctaacttcca tgagagggta cgtagcagat
    13081 cggcaaagac accggcagcg gtaacgtcat tgcccgcacc atatccgcgc agcaccaacg
    13141 gcagcggctg ataatagtgg ctataaaagg ccagggcgtt ttcgccattt ttcactttga
    13201 acagcggatc attcccatcc acttcggcaa tcttcacgcg gcagacacca tcttcatcaa
    13261 tattgccaac atagcgcaaa acttttcctt catcacgggc cttcgccacg cgcgcggcaa
    13321 agagatcgtc gagctgtgac agattcgcca taaaagcggc aacatcaccc tcggcgttaa
    13381 actctgcggg cagcacaggt tcaatttcaa tatccgccag ctccagttca cgtcccgttt
    13441 cacgagcgag aatcaatagt ttacgcgcca catccatacc agaaagatca tctcgcgggt
    13501 ccggttcggt ataacccatt tcccgcgcca gcgtggtcgc ctcggagaaa ctcatgcctt
    13561 cgtctaactt gccgaagata taagaaagcg aacctgaaag aatgccggag aacttcacca
    13621 attcatcacc tgcattgagc agattttgca ggttctcaat aaccggtaat ccagccccaa
    13681 cgttggtgtc atagaggaat ttacgcctcg atttttccgc cgcataacgc aactgatgat
    13741 agtaatccat cgacgaggtg ttggcctttt tgttcggcgt gacaacgtgg aaaccttcgc
    13801 gcaggaagtc ggcatattga tccgccactg cctggctgga ggtgcagtca acaatgaccg
    13861 ggttcagcag atgatattct ttcacgaggc gaattaagcg cccgagatta aacggctctt
    13921 tggcttgtgc aagttcttcc tgccagtttt ccagattaag gccatgcaca ctagtgagca
    13981 gagccttcga gttggcaacg ccgcagacac gtaagtcgat atgtttattc ttcagccagc
    14041 tttgctgacg cttcagttgc tccagcagcg caccgccaac gccaccgacg ccaatcacaa
    14101 acacttcgat aacctgatcg gtattgaaca gcatctgatg agtaacgcgc acgccagtgg
    14161 tcgcatcatc gttatttacc acgacagaga ttgagcgttc agaagatccc tgagcaatgg
    14221 cgacaatgtt gatattggcg cgggccagcg cggcaaagaa tttcgccgag atcccacgca
    14281 aggtgcgcat accatcacct accaccgaga taatggccag ccgttccgtc accgccagcg
    14341 gctccagtaa gccttctttc agttccaggt agaactcttc ctgcattgcc cgttcagctc
    14401 gcacacagtc gctttgtgga acgcagaaac tgatactgta ttcggaagat gattgcgtaa
    14461 tcagcaccac ggaaatacgg gcgcgtgaca ttgcagcaaa gacgcgcgcc gccatgccga
    14521 ccattccttt catccccgga ccagaaacgc tgaacattgc catattattc agattggaaa
    14581 tgcctttgac cggtaattcg tcttcatcac ggctggcacc aatgagcgta cctggtgctt
    14641 gaggatttcc ggtattttta atcaggcaag ggatctggaa ctgggcgatg ggggtaatgg
    14701 tgcgggggtg aagaacttta gcgccgaagt aggaaagctc catcgcttcc tggtaggaca
    14761 tcgactttaa caacctcgca tcgggcacct gacgcgggtc gcaggtatag accccgtcaa
    14821 cgtccgtcca aatctcgcaa caatcggcgc gtaaacaggc agccagcacc gccgcggagt
    14881 agtcggaacc gttgcgtcca agtaccacca gttcgccttt ttcattaccg gcggtgaagc
    14941 ctgccatcag caccatgtga tcagccggaa tacgacttgc cgcaatacgg cgggtggact
    15001 cagcaatatc gacggtagat tcgaggtaat gccccactgc cagtagtttt tcgaccggat
    15061 cgataacggt aacgttgtga ccacgcgctt ctaacacgcc ggccataatg gcgatcgaca
    15121 ttttctcgcc acggcaaatc agcgcagcgt tgatgctatc cgggcactgc cccaacaaac
    15181 taatgccatg caggacatgt tttatttggg caaattcctg gtcgacgaaa gttttcaatt
    15241 gcgccagcgg gaatcccggc tgggcggcgg cgagtcccgt caaaagttcg gcaaaaatac
    15301 gttcggcatc gctgatattg ggtaaagcat cctggccgct aatggttttt tcaatcatcg
    15361 ccaccaggtg gttggtgatt ttggcggggg cagagaggac ggtggccacc tgcccctgcc
    15421 tggcattgct ttccagaata tcggcaaccc gcagaaaacg ttctgcattt gccactgatg
    15481 taccgccgaa cttcaacact cgcatggttg ttacctcgtt acctttggtc gaaaaaaaag
    15541 cccgcactgt caggtgcggg cttttttctg tgtttcctgt acgcgtcagc ccgcaccgtt
    15601 acctgtggta atggtgatgg tggtggtaat ggtggtgcta atgcgtttca tggatgttgt
    15661 gtactctgta atttttatct gtctgtgcgc tatgcctata ttggttaaag tatttagtga
    15721 cctaagtcaa taaaatttta atttactcac ggcaggtaac cagttcagaa gctgctatca
    15781 gacactcttt ttttaatcca cacagagaca tattgcccgt tgcagtcaga atgaaaagct
    15841 gaaaaatact tactaaggcg ttttttattt ggtgatattt ttttcaatat catgcagcaa
    15901 acggtgcaac attgccgtat ctcgttgctc taaaagcccc aggcgttgct gtaaccagtc
    15961 gaccagtttt atgtcatctg ccactgccag agtcgtcagc aatgccatga ctcgttcgcg
    16021 taaagcttgc agttgatgtt ggtctgccgt tgtgtcactt ttcgccggtt gttgtattaa
    16081 tgttgctaat tgatagcaat agaccatcac cgcctgcccc agattgagcg aaggataatc
    16141 cgccaccatc ggcacaccag taagaacgtc agccaacgct aactcttcgt tagtcagccc
    16201 ggaatcttcg cgaccaaaca ccagcgcggc atggctcatc catgaagatt tttcctctaa
    16261 cagcggcacc agttctactg gcgtggcata gtaatgatat ttcgcccgac tgcgcgcagt
    16321 ggtggcgaca gtgaaatcga catcgtgtaa cgattcagcc agtgtcggga aaactttaat
    16381 attatcaata acatcaccag atccatgtgc gacccagcgg gtggctggct ccaggtgtgc
    16441 ctgactatcg acaatccgca gttcgctaaa acccatcgtt ttcattgccc gcgccgctgc
    16501 cccaatattt tctgctctgg cgggtgcgac cagaataatc gttatacgca tattgccact
    16561 cttcttgacc aaataaccgc gaacagggtg atcactgtca acttattacg cggtgagaat
    16621 ttacaaattc ttaacgtaag tcgcagaaaa agccctttac ttagcttaaa aaagactaaa
    16681 ctatttcctg actgtactaa cggtttaatt gtt
//
LOCUS       DBANUU010000092        16479 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000092 DBANUU010000000
VERSION     DBANUU010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.092"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(375..1571)
                     /gene="hemY"
                     /locus_tag="ACIE68_003154"
     CDS             complement(375..1571)
                     /gene="hemY"
                     /locus_tag="ACIE68_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFX5909429.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1574..2779)
                     /gene="hemX"
                     /locus_tag="ACIE68_003155"
     CDS             complement(1574..2779)
                     /gene="hemX"
                     /locus_tag="ACIE68_003155"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFX5909430.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDSAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQAD
                     TSAAAPQGE"
     gene            complement(2801..3541)
                     /gene="hemD"
                     /locus_tag="ACIE68_003156"
     CDS             complement(2801..3541)
                     /gene="hemD"
                     /locus_tag="ACIE68_003156"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFX5909431.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3538..4479)
                     /gene="hemC"
                     /locus_tag="ACIE68_003157"
     CDS             complement(3538..4479)
                     /gene="hemC"
                     /locus_tag="ACIE68_003157"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFX5909432.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4866..7412
                     /gene="cyaA"
                     /locus_tag="ACIE68_003158"
     CDS             4866..7412
                     /gene="cyaA"
                     /locus_tag="ACIE68_003158"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFX5909433.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7452..7772)
                     /gene="cyaY"
                     /locus_tag="ACIE68_003159"
     CDS             complement(7452..7772)
                     /gene="cyaY"
                     /locus_tag="ACIE68_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFX5909434.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7934..8076)
                     /locus_tag="ACIE68_003160"
                     /pseudo
     CDS             complement(7934..8076)
                     /locus_tag="ACIE68_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8235..8438
                     /gene="yifL"
                     /locus_tag="ACIE68_003161"
     CDS             8235..8438
                     /gene="yifL"
                     /locus_tag="ACIE68_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFX5909435.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8475..9299
                     /gene="dapF"
                     /locus_tag="ACIE68_003162"
     CDS             8475..9299
                     /gene="dapF"
                     /locus_tag="ACIE68_003162"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFX5909436.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9296..10003
                     /gene="yigA"
                     /locus_tag="ACIE68_003163"
     CDS             9296..10003
                     /gene="yigA"
                     /locus_tag="ACIE68_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFX5909437.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMVLLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            10000..10896
                     /gene="xerC"
                     /locus_tag="ACIE68_003164"
     CDS             10000..10896
                     /gene="xerC"
                     /locus_tag="ACIE68_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFX5909438.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            10896..11612
                     /gene="yigB"
                     /locus_tag="ACIE68_003165"
     CDS             10896..11612
                     /gene="yigB"
                     /locus_tag="ACIE68_003165"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFX5909439.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11696..13858
                     /gene="uvrD"
                     /locus_tag="ACIE68_003166"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11696..13858
                     /gene="uvrD"
                     /locus_tag="ACIE68_003166"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFX5909440.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(14005..14769)
                     /gene="yigE"
                     /locus_tag="ACIE68_003167"
     CDS             complement(14005..14769)
                     /gene="yigE"
                     /locus_tag="ACIE68_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFX5909441.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLI
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKEIQFAVQSGP
                     MLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFSCYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            14931..14987
                     /gene="ysgD"
                     /locus_tag="ACIE68_003168"
     CDS             14931..14987
                     /gene="ysgD"
                     /locus_tag="ACIE68_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFX5909442.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            15139..16089
                     /gene="corA"
                     /locus_tag="ACIE68_003169"
     CDS             15139..16089
                     /gene="corA"
                     /locus_tag="ACIE68_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFX5909443.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(16132..>16479)
                     /gene="yigF"
                     /locus_tag="ACIE68_003170"
     CDS             complement(16132..>16479)
                     /gene="yigF"
                     /locus_tag="ACIE68_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFX5909444.1"
                     /translation="SEKWKYRFNFYDQHGFPGFWGATPEYKAAFKALKVRQRLTIQMN
                     FIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVAAYVAVNTNKWF
                     YEKEVKGLNTWSL"
BASE COUNT         3778 a         4282 c         4428 g         3991 t
ORIGIN      
        1 agctcaggtt aaatattcac tcaggaagtt attactcagg aagcaaagag gattacagaa
       61 ttatctcata acaagtgtta agggatgtta tttcccgatt ctctgtggca taataaacgg
      121 gtagatgctc attccatctc ttatgttcgc cttagtgcct cataaactcc ggaatgacgc
      181 agagccgttt acggtgctta tcgtccactg acagatgtcg cttatgcctc atcagacacc
      241 atggacacaa cgttgagtga agcacccact tgttgtcata cagacctgtt ttaacgcctg
      301 ctccgtaata agagcaggcg tttttttatg catcagaaag gtcccggagg tgcttgcctc
      361 cgggtgagaa ggaactactg tggtgggttg ttctgcaacg ttaacatcaa accgtcgcga
      421 cgcatcgctg cagcttcttc cggcttgtgc agtctgtcca gcgcgtcggc aagccatgcg
      481 taatcgtagg cgtccggacg ttgtttcagc gccgcgcgga aggcgagcga tgcttcctgc
      541 cattctccgt gcttcatcag tgactggccc agtgtgctcc acaacagcgg gcgatcgccg
      601 acgtttttga tctgctggcg tagcactttt tccagctgtt ccgggttatt ggttttcaga
      661 cgcgggatcg gcagcagtag gcgatcgtca tactggcgtt tcaggccgtc gatgataatt
      721 tgctgggcag tatcatgatc gtcacattca ataagatgtt ccgccattgc cacctgcaac
      781 gctacctgat gacgcgtttt ccggctttgg tttttccacc agttacgcaa accttcgcta
      841 ccgttatcgg cacgagcctg atccatcagg ccaatccatg cctgttgttc cagcattgcg
      901 cgatgttctt catcaccaac atgggctttc gccattgatg ggataatatc cagcagcgaa
      961 ctccatgcac ctgtgcggat atacgcctgt tccgccagac gtaatacttc cggatggcgt
     1021 ggcgtaactt ccagcagctt atccacgccg tggcgtgcag catggttttc attacgggcc
     1081 agttgcagac gtacacgggt gatttctacc ggaatggtgt cattgccggc cagctccgct
     1141 gcgcgttcca gatgttggtt ggcgcgtgct tcatcaccac gttgttgcgc ggcttcggca
     1201 gccagtagat agttcaccac cggttgttcc gcgtgatcgg catttttcgc catcagcttt
     1261 tcaacttgct gataatcgcc ttccgccagt ttcagcagcg cctgttcggt ctgcttacgg
     1321 gcacggcgac gcttacgtcc gacaaaccac ccacgggtgt gcgcgccagt gcggaagatc
     1381 cgccgcagta gccactcaat ggcaaacagc actaccatcg ccagaatcaa aataatcgcc
     1441 aggcccgtga cgctggtttc gatattgtag ttgtcggtct ggatcagcac ataaccctga
     1501 tgaccggcaa tcatcgggcc aaccacgatc cccgcaatca gcaacacaaa gagcaataac
     1561 acttttagca tggttattct ccttgcggcg cagctgccga agtatcagct tgcggtgcag
     1621 gtgcaggtgc aggtgcaggt gcaggtgcag gtttagcttc cgttgtcccc gctgccggtt
     1681 gtgccagcag gttacgcacg cgagtctgca tcagtttttc cagcatcgcc tggctttgca
     1741 gggtttccgg aagatccatc gagatatttt gctggcttaa ctggtccacc tcgtcgagga
     1801 acgctttggt ggtggcatca tcagtatcgt agtaagcacg tacccaggtg gagacgttct
     1861 ccagcgcctg gcgataagtc tcttgctggt gacgcggtac agcttgtgct gcgaccagca
     1921 ggcgagagcg aatattttcg cgcagataga tatcctgatt tggcgctaac agcggtacgg
     1981 cggagtcatc acgacggcga atcgtaatga agttgtccat aaaattctgc cagctttttt
     2041 gcagattgat acgccattcg ctgatggaac tggaaagctc ttcaccgtcg gaatccatcg
     2101 gcgaaccatc gctgtcatta tcggccagac gcaggttatc tacctgattt gaaagctgat
     2161 tgagcttaag gatgatgccg tcataatcca cctgcgatac cgcagaaagg ctggcgatat
     2221 catcggtaat tgcccggcga acggtaatca gactcgggtc attcatatcc gccaggctgg
     2281 catctgcact tttcagcaac gctgcagcgg tcgtgacgtc ttgatcgctc cacagcttcc
     2341 gtccggcgag tttcaccaga aaatcggcct gagccagcag ccaggtttta gcatcgctgc
     2401 cggaaatggt ggcgaccttt tgttggactt catccaactg ttttgccagc gtttcttgct
     2461 gacgattcgc ctgcttaagc tgtgcagctt gttgcttaat aatgccttcc agctcggctt
     2521 tttggctctc ctgggctttt tgcaatgccg tcagttggtt agccagggca tcgctggtgg
     2581 cggtctgatt gacggcctgt tgtttacccc agccatacaa accgacgccc gccgccagag
     2641 caatagcgat agccaccgcg ctgagaatca atgcggtatt gttcttactc tttttttctg
     2701 ttgcgacagg ttgtgacgtg gtgtccacgg cctccctggt ctcttcaacc acggcggagg
     2761 ttttttcttg ttccgtcatt atggcttcct gttatgagag ttattgtaat gcccgtaaaa
     2821 gcgcatcgtt gtcagcgtta tcggcgacct taatgtcttg ccagcccagt tcccgggcga
     2881 gtttcgccaa acgctcactg acgaccaata gtcgacagtg tagtaaccag tgctcacgat
     2941 accattgtgg gatcagcgac cagagttgct gcaacatttc accgctggta acaacgaccg
     3001 tcgtcacctc gcgggattgc cagcgcatcg cttcttctgc accatcgtaa tggattgcgc
     3061 atcgttgata acattcacaa aaagtgacct cagcaccgcg cgccgtcagg gtatccccaa
     3121 ttagctcacg accgccattg ccacgtaata tcagcgcacg tttgcccgca atattttgta
     3181 attcaggtaa ttgtagcaag acttcgctga tttcccgatc ctgcgggtag agaatcttct
     3241 gtccgcttac ggtatgtagt gccagtgcgg tggtgcgtcc aatggcgaaa taattaggta
     3301 gtcggggcca tttacgatct tgctgatgta gctgtgattg ggcaaaagca accgcgtgtt
     3361 gcgagagggc aaacaacaga tcgctctccc ccagcgctgc cagttgatca gcaagttgcg
     3421 gtaattgtcg acccggagaa aactcaatca gcggaaaatg ccaggccacc tgtcccagtg
     3481 tgcgcagacg gctcactaac tcttctccag cgggagacgg gcgggtgacc aggatactca
     3541 tgccggggcg tctccgttat agacttcagc gaggatctcg cgcgcgccgt tattcagtag
     3601 ctcttctgcc agcgaaatcc ccatttgttc ggcatcttgc ggcgcaccgc ggcgttcacc
     3661 gcgaataatc tgcgaaccgt ccggcgcgcc gaccagcgca cgtagccaga tttcgccatc
     3721 aataagctcg gcgtagctac caattggcac ctgacatccg ccttcgagac gggtattcat
     3781 ggcgcgttct gcggtaacgc gcagtgcagt ttcgtggtga ttcagcgcgg caagcagctc
     3841 gcgagtgcgt gcatcatcaa ggcggcattc aatacccacc gcaccttgcc ctaccgccgg
     3901 aagagaaatc tcgggcggta acgcggcgcg aatgcgggac tccagaccta aacgttttag
     3961 tccggctacg gcaagaatga tggcatcgta ttcgccgtta tccagtttac tcaggcgagt
     4021 gccgacgttg ccgcgcaggg agcggataat cagatccggg cggcgttcag ccagttggca
     4081 ctggcgacgt aaactggacg tcccgacgat actgcctgcc ggtaacgcat ccagactgtc
     4141 atagttattg gacacaaagg catcgcgagg atcttcacgc tcacaaatag tgaccagtcc
     4201 cagaccttgc ggaaattcaa ccggcacatc tttcattgag tgtacggcga tatcggcgcg
     4261 attttcgagg agcgcgactt ccagctcttt tacaaataag ccttttccac ctactttcgc
     4321 cagcggcgta tcaagaatca catcgccgcg cgtcaccatc ggtaccagtt caacgaccag
     4381 gcccggatgg ctcgccatca acttgtcttt gacatagtgt gcctgccaga gtgcaagtgg
     4441 gctttggcgt gtggcaattc ttaaaacatt gtctaacatg cttgttaccg tcattatcat
     4501 ccgtggttca tcctaacatc cttgccagag tgatgtcagt gttgtggtga aacgtagacg
     4561 cctgcgcaaa ccgtaaaatg aggtctggca gtggatcctg acaggcgttt cacgccgtcg
     4621 taataaggaa tttacagaga ataaacggtg ctacacttgt atgtagcgca tctttcttta
     4681 cggtcaatca gcaaggtgtt aaattgatca cgttttagac cattttttcg tcgtgaaact
     4741 aaaaaaacca ggcgcgaaaa gtggtaacgg ttacctttga catacgaaat atcccgaatg
     4801 ccgcgtgtta ccgttgatgt tggcggaatc acagtcatga cgggtagcaa atcaggcgat
     4861 acgtcttgta cctctatatt gagactctga aacagagact ggatgccata aatcaattgc
     4921 gtgtggatcg cgcgcttgct gctatggggc ctgcattcca acaggtctac agtctactgc
     4981 cgacattgtt gcactatcac catccgctaa tgccgggtta ccttgatggt aacgttccca
     5041 aaggcatttg cctttacacg cctgatgaaa ctcaacgcca ctacctgaac gagcttgaac
     5101 tgtatcgtgg aatgtcagta caggacccgc cgaaaggtga gcttccaatt actggtgtat
     5161 acaccatggg cagcacctcg tccgtagggc aaagttgttc ctctgacctg gatatctggg
     5221 tctgtcatca atcctggctc gatagcgaag agcgccagtt gctacaacgt aaatgtagtc
     5281 tgctggaaag ctgggccgcc tcgctgggtg tggaagtcag cttcttcctg attgatgaaa
     5341 accgcttccg tcataatgaa agcggcagcc tggggggcga agattgtggc tccacccagc
     5401 atatactgct gcttgacgaa ttttatcgta ccgccgtgcg tctcgccggt aagcgtattc
     5461 tgtggaatat ggtgccgtgc gacgaagaag aacattacga cgactatgtg atgacgcttt
     5521 acgcgcaggg cgtgctgacg ccaaatgaat ggctggatct cggtggctta agctcgcttt
     5581 ctgctgaaga gtactttggt gccagccttt ggcagctcta caagagtatc gattccccat
     5641 acaaagcggt gctgaaaaca ctgctgctgg aagcctattc ctgggaatac ccgaacccac
     5701 gtctgctggc gaaagatatc aaacagcgtt tgcacgacgg agagattgta tcgtttggtc
     5761 tcgatccata ctgcatgatg ctggagcgtg ttactgaata cctgacggcg attgaagatt
     5821 ttacccgtct ggatttagta cgtcgctgct tctatttaaa agtgtgcgaa aagctcagcc
     5881 gtgaacgcgc ctgcgtaggc tggcgtcgcg cagtgttgag ccagttagtg agcgagtggg
     5941 gttgggacga agctcgtctg gcaatgctcg ataaccgcgc taactggaag attgatcagg
     6001 tgcgtgaggc gcacaacgag ttgctcgacg cgatgatgca gagctaccgt aatctgatcc
     6061 gctttgcgcg tcgcaataac cttagcgtct ccgccagtcc gcaggatatc ggcgtgctga
     6121 cgcgtaagct gtatgccgcg tttgaagcat taccaggtaa agtgacgctg gtaaacccgc
     6181 agatttcacc cgatctctcg gaaccgaatc tgacctttat ttatgtgccg ccgggccgtg
     6241 ctaaccgttc aggttggtat ctgtataacc gcgcgccaaa tattgagtcg atcatcagcc
     6301 atcagccgct ggaatataac cgttacctga ataaactggt ggcgtgggca tggtttaacg
     6361 gcctgctgac ctcgcgcacc cgtttgtata ttaaaggtaa cggcattgtc gatttgccta
     6421 agttgcagga gatggtcgcc gacgtgtcgc accatttccc gctgcgctta cctgcaccga
     6481 caccgaaggc gctctacagc ccgtgtgaga tccgccatct ggcgattatc gttaacctgg
     6541 aatatgaccc gacagcggcg ttccgcaatc aggtggtgca tttcgatttc cgtaagctgg
     6601 atgtcttcag ctttggcgag aatcaaaatt gcctggtagg tagcgttgac ctgctgtacc
     6661 gcaactcgtg gaacgaagtg cgtacgctgc atttcaacgg cgagcaatcg atgatcgaag
     6721 ccctgaaaac tattctcggc aaaatgcatc aggacgccgc accgccagat agcgtggaag
     6781 tcttctgtta tagccagcat ctgcgcggct taattcgtac tcgcgtgcag caactggttt
     6841 ctgagtgtat tgaattgcgt ctttccagca cccgccagga aaccgggcgt ttcaaggcgc
     6901 tgcgcgtttc tggtcaaacc tgggggttgt tcttcgaacg cctgaatgta tcggtacaga
     6961 aactggaaaa cgccatcgag ttttatggcg cgatttcgca taacaaactg cacggcctgt
     7021 cagtgcaggt tgaaaccaat cacgtcaaat taccggcggt ggtggacggc tttgccagcg
     7081 aagggatcat ccagttcttt ttcgaagaaa cgcaagacga gaatggcttt aatatctaca
     7141 ttctcgacga aagcaaccgg gttgaggtgt atcaccactg cgaaggcagc aaagaggagc
     7201 tggtacgtga cgtcagtcgc ttctactcgt catcgcatga ccgttttacc tacggctcaa
     7261 gcttcatcaa cttcaacctg ccgcagttct atcagattgt gaaggttgat ggtcgtgaac
     7321 aggtgattcc gttccgcaca aaatctatcg gtaacatgcc gcctgccaat caggatcacg
     7381 atacgccgct attacagcag tatttttcgt gatgaacgtg ccggaaagcg aggcttatcc
     7441 ggcatgcaat cttagcggaa actgactgtt tcacccgcct gctgcgtcgc cgcctgttcc
     7501 agcaaatccc agaaggtttc gccgctgcga tcgcaaatcc actcatcgcc tttcagatca
     7561 aaatggtagc cgccttgttt ggttgccagc catacctggt gcagcggttc ctgtcggttg
     7621 ataatgattt tgctgccgtt ttcaaaggta atggtcagta cgccgccgtt gatttcgcag
     7681 tcgatatcgc tgtcgccatc ccagtcgtcc aggcgttctt caatggtcag ccagagttga
     7741 tcagccaggc gatgaaattc actgtcgttc attgttgtat cctgttttta agtgatggcg
     7801 gcagtatagc ggcatggggt cagggcttca aagtttgcac ctctgcggct gcgttccggc
     7861 acgattcatc cgtcaccgga ataatgatgt ctcagtgtag cgaaagattt gtctcttcat
     7921 tagggcgcag ttacaccacg cctttccctg tttctggtaa acattatgat caggtttacc
     7981 gagcgagcat cctcacgctg acggaactaa aaaagccaac aaacaaacca cattgcgata
     8041 gtgcataaag ccatcctggc gcgaggtgtt gatcacgaaa ctgccagcag aacataaatc
     8101 cccacgagta agcgttatac tcgcagcatt tcctcacttt tcagacttca taaagagtcg
     8161 ctaaatgctt gcttttacgc cttctcctgc gatgatagaa agcagaaagc gatgaacttt
     8221 acaggcaatc cataatgaaa aacgtgttta aggcactcac tgtattactt actctcttca
     8281 gcctgacggg ctgcggtctg aaaggtccgc tctatttccc gcctgcagat aaaaatgcac
     8341 cgccgccgac caaaccggta gagacgcaaa cgcaatccac ggtgccggat aaaaacgatc
     8401 gcgccactgg cgatggtcca tcccaggtga attactaaaa gtcagtttct gtacccgcgt
     8461 gattggagta aatgatgcag ttctcgaaaa tgcatggcct tggcaacgat tttatggtcg
     8521 tcgacgcggt aacgcagaat gtcttttttt caccggagct gattcgtcgc ctggcggatc
     8581 ggcacctggg ggtagggttt gaccagctgc tggtggttga gccgccgtat gatcctgaac
     8641 tggattttca ctatcgcatt ttcaatgctg atggcagtga agtggcgcag tgcggcaacg
     8701 gtgcgcgctg ctttgcccgt tttgtgcgcc tgaaagggct taccaataag cgcgatatcc
     8761 gcgtcagcac cgccaacggg cggatggttc tgaccgtcac cgatgatgat ttggtccgcg
     8821 taaatatggg cgaacccaac ttcgaacctt ccgctgtgcc gtttcgcgct aacaaagcgg
     8881 aaaagaccta tattatgcgc gccgccgagc agacaatctt atgcggcgtg gtgtcgatgg
     8941 gaaatccgca ttgcgtgatt caggtcgatg atgtcgatac cgcggcggta gaaacgcttg
     9001 gtcctgttct ggaaagccac gagcgttttc cggagcgagc caatatcggt tttatgcaag
     9061 tggttaagcg cgagcatatt cgtttacgcg tttatgagcg tggggcagga gaaacccagg
     9121 cctgcggcag cggcgcgtgt gcggcggttg cagtagggat tcagcaaggt ttgctggccg
     9181 aagaagtacg cgtggaactc cccggcggtc gtcttgatat cgcctggaaa ggtccgggtc
     9241 acccgttata tatgactggc ccggcggtac atgtctacga cggatttatt catctatgaa
     9301 gcaaccaggg gaagaactgc aggaaacact cacggagctt gatgaccggg cggttgtcga
     9361 ttatctgatt aaaaatcctg agttttttat ccgcaatgcg cgcgcagtag aagcgatacg
     9421 tgtgccgcat ccggtacgcg gcaccgtttc gttggtcgag tggcacatgg cccgcgcacg
     9481 taatcatatt catgttctgg aagagaacat ggtgctgttg atggaacagg ctatcgccaa
     9541 cgaaggcctg ttttatcgcc tactctacct gcagcgcagt ctcaccgccg ccagcagtct
     9601 cgacgatatg ctgatgcgct ttcaccgctg ggcgcgcgat ctcggcctgg caggtgcgag
     9661 tctgcgcctg tttccggatc gctggcgctt aggtgcgccg tcgaaccaca ctcatctggc
     9721 attaagccgt cagtctttcg aaccgctgcg tattcagcgt ttggggcagg aacagcacta
     9781 tcttgggccg cttaacggac cagagctgct ggtggtgcta ccggaagcga aagcggtggg
     9841 atcggtggcg atgtcgatgc tgggaagcga tgctgatttg ggtgtcgtgc tgtttaccag
     9901 tcgtgatgcc agtcactatc aacaagggca gggaacgcag ttacttcatg aaattgcgct
     9961 gatgttgccg gagcttctgg agcgttggat tgaacgcgta tgaccgattt acacaccgat
    10021 gtagaacgct acctacgtta tctgagcgtg gagcgtcagc ttagcccgat aaccctactt
    10081 aactaccagc gtcagcttga ggcgatcatc aattttgcca gcgaaaacgg cctgcaaagc
    10141 tggcaacaat gcgatgcagc gatggtacgc aattttgctg tacgcagtcg ccgtaaaggg
    10201 ctgggagcag caagtctggc gttacggctt tctgcgctac gtagcttttt tgactggctg
    10261 gtcagccaga acgaactcaa agctaacccg gcgaaaggag tttcggcacc gaaagcgccg
    10321 cgtcatctgc cgaaaaacat cgacgtcgac gatatgaatc ggctgctgga tattgatatc
    10381 aacgatcccc tcgctgtacg cgaccgtgca atgctggaag tgatgtacgg cgcgggtctg
    10441 cgtctttccg agctggtggg gctggatatc aaacacctcg acctggagtc tggtgaagtg
    10501 tgggtaatgg ggaaaggcag caaagagcgc cgcctgccga ttggtcgcaa cgctgtggcg
    10561 tggattgagc actggcttga tttgcgcgac ctgtttggta gcgaagacga cgcgcttttt
    10621 ctgtcgaaac tgggcaagcg tatctccgcg cgtaatgtgc agaaacgctt tgccgaatgg
    10681 ggcataaaac aagggctgaa taatcacgtt cacccgcata aattacgtca ctcgttcgct
    10741 acgcatatgc tggagtcgag cggcgatctt cgtggtgtgc aggagctgct gggtcatgcc
    10801 aacctctcca ccacgcaaat ctatactcat cttgattttc aacaccttgc ctcggtgtac
    10861 gatgcggcgc atccacgcgc caaacggggg aaataatgcg tttttaccgg cctttggggc
    10921 gcatctcggc gctcaccttt gacctggatg atacccttta cgataaccgt ccggtgattt
    10981 tgcgcaccga gcgagaggcg cttacctttg tgcaaaatta tcatccggcg ctgcgcagct
    11041 tccagaatga agatctgcaa cgcctgcgcc aggcggtacg ggaagcggaa cccgagattt
    11101 atcacgacgt gacgcgctgg cgttttcgtt cgattgaaca agcgatgctc gacgccgggc
    11161 tgagtgccga agaagccagt gcaggcgcac acgcagcaat gatcaacttt gccaaatggc
    11221 gcagccgaat cgacgtcccg cagcaaactc acgacacctt aaaacagctg gcgaagaaat
    11281 ggccgctggt ggcgatcacc aacggtaacg cccagccgga gctgtttggt ttgggggatt
    11341 attttgagtt tgtgctgcgc gctggcccgc acgggcgctc aaaaccgttc agcgatatgt
    11401 actttttggc tgcggaaaaa ctcaacgtgc cgatcggcga gatcttacat gttggggacg
    11461 atctcaccac tgacgtgggt ggggcaattc gcagcggaat gcaggcttgt tggatcagac
    11521 cggaaaatgg cgatctgatg caaacctggg acagccgttt actgccgcat ctggaaattt
    11581 cccggttggc atctctgacc tcgctgatat aatcagcaaa tctgtatata tacccagctt
    11641 tttggcggag ggcgttgcgc ttctccgccc aacctatttt tacgcggcgg tgccaatgga
    11701 cgtttcttac ctgctcgaca gccttaatga caaacagcgc gaagcggtgg ccgcgccacg
    11761 cagcaacctt ctggtgctgg cgggcgcggg cagtggtaag acgcgcgtac tggtgcatcg
    11821 tatcgcctgg ttgatgagcg tggaaaactg ctcgccatac tcgattatgg cggtgacgtt
    11881 taccaacaaa gcggcggcgg aaatgcgtca tcgtatcggg caactgatgg gcaccagcca
    11941 gggcggcatg tgggtcggca ccttccacgg gctggcgcac cgtttgctgc gtgcgcacca
    12001 tatggacgcc aatctaccgc aggatttcca gatcctcgac agtgaagacc agctacgcct
    12061 gcttaagcgt ctgatcaaag ccatgaacct cgacgagaag cagtggccgc cgcgccaggc
    12121 aatgtggtac atcaacagcc agaaagatga aggcctgcgt ccgcatcata ttcaaagcta
    12181 cggtaatccg gtggagcaga cctggcagaa ggtgtatcag gcgtatcagg aagcgtgcga
    12241 tcgtgcgggc ctggtggact tcgccgagct gctgctgcgc gctcacgagt tgtggcttaa
    12301 caagccgcat atcctgcaac actaccgcga acgttttacc aacatcctgg tggacgaatt
    12361 ccaggatacc aacaacattc agtacgcgtg gatccgcctg ctggcgggcg ataccggcaa
    12421 agtgatgatc gttggcgatg acgaccagtc aatctacggc tggcgcgggg cgcaggtaga
    12481 gaatattcag cgtttcctta atgatttccc cggtgccgaa actattcgtc tggagcaaaa
    12541 ctaccgctct accagcaata ttctgagcgc ggctaacgcc ctgattgaaa ataacaatgg
    12601 gcgcttaggt aaaaaattgt ggaccgatgg cgcggacggt gagccgattt ctctttattg
    12661 cgcctttaac gaactcgacg aagcgcgttt tgtggttaac cgcatcaaaa cctggcagga
    12721 taacggcgga gcgcttgccg agtgcgccat tctctaccgc agtaacgccc agtcgcgcgt
    12781 gctggaagaa gcactgttgc aggcgagtat gccgtaccgt atttacggcg ggatgcgctt
    12841 cttcgaacgc caggaaatca aagatgcgct ctcgtatctg cgcctgattg ccaaccgcaa
    12901 cgacgacgcg gcctttgagc gcgtagtgaa tacaccaacg cggggtattg gtgaccggac
    12961 gctggacgtg gtacgtcaga catcgcgcga tcgccagtta acgctgtggc aggcgtgccg
    13021 tgaactgttg caggaaaaag cgctcgccgg acgtgcagcc agcgccttgc agcggtttat
    13081 ggaactgatc gacgccttag cgcaagaaac tgccgatatg ccgctacatg tacagactga
    13141 ccgggtcatt aaagactccg gcctgcgcac catgtacgag caggagaagg gcgaaaaagg
    13201 tcagacgcgt atcgaaaact tggaggaact ggtgacggca acgcgccagt tcagctacaa
    13261 cgaagaagat gaagatttaa tgccgttgca ggcgtttctc tcccatgcgg cgctggaagc
    13321 gggcgaaggg caggcggata cctggcagga cgcggtgcag ttgatgacgc tacactcggc
    13381 gaaaggtctg gagtttccgc aggtgtttat tgtcggtatg gaagagggca tgttcccaag
    13441 ccagatgtcg ctggatgaag gcgggcgtct ggaagaagaa cgccgtctgg cctacgttgg
    13501 cgtaacccgc gcgatgcaga aactgacgtt gacctacgcg gaaactcgcc gtctgtatgg
    13561 caaagaggtt taccatcgcc cgtcgcgctt tatcggcgag ctgccggaag agtgtgtgga
    13621 agaggtgcgc ctgcgcgcta cggtaagtcg tccggtcagc catcaacgga tggggacgcc
    13681 gatggtcgag aacgacagcg gctacaagct cggtcagcgt gtacgccacg ctaagtttgg
    13741 tgaaggcacc atcgtcaata tggaaggcag cggtgaacat agccgtttgc aggtggcgtt
    13801 ccagggccag ggaatcaaat ggctggtggc ggcttacgcc cggctggaga cggtgtaacg
    13861 ttgccggatg cggtgctgcg caccttattt ggcctaaaaa atcattcaga ttcaataaat
    13921 tgcaacgtca tgtaggccgg ataaggcgtt tacgccgcat ccggcatcag cagcatattt
    13981 agtatctgac acaaaactat cattctaacc tttccgctcg acggaaatca tggtgacaaa
    14041 aggatagcgt tgccacggga ttgccccgcc tttcatatac atatgtgaaa tcgtgccatc
    14101 aagataaagc agctgctcaa cgttcagttt cgctttggca taacaggaaa aatcataaaa
    14161 atttgttgcc tgctggctca acaaaaacac ggcattccca tgtttattaa tcccaacacc
    14221 gttacgaatt ttacgtgagg cgacgttggg atgaatacgc ggattaatta caccgttttc
    14281 catcaacatt ggccctgact gcaccgcaaa ctgaatctct ttactggctt taaaggcatc
    14341 cagacgaacg atgccgactt tatctcccgc gacataaaac acgccgccag gacggataaa
    14401 gaaattccct tcacctgaag cgagatttaa cgccaccttc tgctgaccgt tttcgatgta
    14461 caaaccgagc ggcgcatagc tttcatcata gatgccgccg ttcatcgcca tctgcacctg
    14521 accctgacta ttcatatcca ccagcagagc atgtaaagtt ccccacgctt cgccattggc
    14581 tttttgccag tacattttca cccgctctgt ttgaggatta acggtatacg cctgtacgat
    14641 cagcatcgga tctgagagtg cgcaatcatc agcggcaaca gcaaacaagg gaagtaaggt
    14701 gagggcgaga aaaatccgtt tgagattcaa ggtgatcatt cctttaccaa tgagtagccg
    14761 atgcgccatt ataggtcctg gatgtgggat ttttttatcc tgttagcgac cttgacgagt
    14821 accaaaaagc gcgaagttca actattgttc tgtggtgttc tgttgcgtgt tgacggcaaa
    14881 attttgctgg cgtaacatgc gcgcacgatc actctaagag gacattcgcc ttggacacac
    14941 ccagtagata ctggctcact atcctgtcat ccaggatcaa ctcctaaggc tatccctttt
    15001 tgctgatagc cttagcggtt gtcagcgacc tcaatttttc ccgtcgcgct gagtcaggct
    15061 gtttaatggt ctgaaaccca atttgtttct gtgtgcccac cgaactgtcc gatattttaa
    15121 gcattgggag tcccggtcat gctgagcgca tttcaactgg aaaataaccg actgacccgg
    15181 ctggaagtcg aagagtcaca accccttgta aatgcagtat ggattgatct tgtcgaaccg
    15241 gacgacgacg agcgactgcg cgtacaatct gaacttggcc agagcctggc aacccgcccg
    15301 gaactggaag acatcgaagc atcggcacgt ttctttgaag acgacgacgg cctgcatatt
    15361 cactccttct tcttctttga agatgcggaa gatcacgccg gtaactccac tgtggcattt
    15421 accatccgtg atggtcgtct gtttactctg cgtgagcgtg aactgcccgc ttttcgtctg
    15481 tatcgtatgc gtgcccgtag ccagtcgatg gtagacggta acgcctacga gctgctgctg
    15541 gatctgttcg aaaccaaaat cgaacagttg gcagatgaaa ttgaaaatat ctatagcgac
    15601 ctggagcagt tgagccgggt gattatggaa gggcatcagg gcgatgagta cgacgaggcg
    15661 ctctccactc tggcggaact ggaagatatc ggctggaaag tgcgcctgtg tctgatggat
    15721 acccagcgtg cgctcaactt cctggtgcgt aaagcgcgtt taccgggtgg gcaactggag
    15781 caggcgcgtg aaatcctgcg agatatcgaa tccctgctgc cgcataacga atccctgttc
    15841 cagaaggtga acttcctgat gcaggcggca atgggtttta tcaacatcga gcagaaccgc
    15901 atcatcaaaa ttttctcggt ggtatccgtc gtgttcctgc cgccaacgct ggttgcttcc
    15961 agctatggga tgaactttga gtttatgcca gaactgaagt ggagtttcgg ctaccctggc
    16021 gcgattatct ttatgatcct cgcgggcctg gcaccgtatc tgtactttaa gcggaagaac
    16081 tggttgtaaa aaacgagagc ggtggcttag tctggctaag ccacctgtta ttcaaaggct
    16141 ccaggtattt aaccctttta cctctttctc atagaaccat ttgttcgtgt taacagcaac
    16201 ataggctgct acggcaattc ccagaatgtt aacgccaatt aatgcaccaa caaatagaga
    16261 tagaatgcca agtaatagaa ctataattgc ttttttccat aagcccagaa caaataaata
    16321 gatccaggaa cagaagaaag caatgaaatt catttgaata gttaatcgtt gtcttacttt
    16381 taatgcttta aatgctgctt tatattctgg tgttgctccc cagaatccag gaaagccatg
    16441 ttgatcataa aaattaaatc ggtatttcca tttttcact
//
LOCUS       DBANUU010000093        16413 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000093 DBANUU010000000
VERSION     DBANUU010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.093"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(37..189)
                     /locus_tag="ACIE68_003171"
     CDS             complement(37..189)
                     /locus_tag="ACIE68_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFX5909445.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(454..1188)
                     /gene="cysH"
                     /locus_tag="ACIE68_003172"
     CDS             complement(454..1188)
                     /gene="cysH"
                     /locus_tag="ACIE68_003172"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFX5909446.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMLEEETRFFGLKRECGLHEG"
     gene            complement(1262..2974)
                     /gene="cysI"
                     /locus_tag="ACIE68_003173"
     CDS             complement(1262..2974)
                     /gene="cysI"
                     /locus_tag="ACIE68_003173"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFX5909447.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2974..4773)
                     /gene="cysJ"
                     /locus_tag="ACIE68_003174"
     CDS             complement(2974..4773)
                     /gene="cysJ"
                     /locus_tag="ACIE68_003174"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFX5909448.1"
                     /translation="MTTQVPPSALLPLNPEQLVRLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5092..5454
                     /gene="queD"
                     /locus_tag="ACIE68_003175"
     CDS             5092..5454
                     /gene="queD"
                     /locus_tag="ACIE68_003175"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFX5909449.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5532..6803
                     /gene="ygcN"
                     /locus_tag="ACIE68_003176"
     CDS             5532..6803
                     /gene="ygcN"
                     /locus_tag="ACIE68_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFX5909450.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERCITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            6794..7054
                     /gene="ygcO"
                     /locus_tag="ACIE68_003177"
     CDS             6794..7054
                     /gene="ygcO"
                     /locus_tag="ACIE68_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFX5909451.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTAERLISACPAGLFSLTPE
                     GDLRIDYRSCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7071..7646
                     /gene="ygcP"
                     /locus_tag="ACIE68_003178"
     CDS             7071..7646
                     /gene="ygcP"
                     /locus_tag="ACIE68_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFX5909452.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7794..8654)
                     /gene="ygcQ"
                     /locus_tag="ACIE68_003179"
     CDS             complement(7794..8654)
                     /gene="ygcQ"
                     /locus_tag="ACIE68_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFX5909453.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLIIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPKVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8651..9430)
                     /gene="ygcR"
                     /locus_tag="ACIE68_003180"
     CDS             complement(8651..9430)
                     /gene="ygcR"
                     /locus_tag="ACIE68_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFX5909454.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWRDYLRQRMQP"
     gene            complement(9408..10817)
                     /gene="ygcS"
                     /locus_tag="ACIE68_003181"
     CDS             complement(9408..10817)
                     /gene="ygcS"
                     /locus_tag="ACIE68_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5909455.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10839..12293)
                     /gene="ygcU"
                     /locus_tag="ACIE68_003182"
     CDS             complement(10839..12293)
                     /gene="ygcU"
                     /locus_tag="ACIE68_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFX5909456.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12363..13148)
                     /gene="ygcW"
                     /locus_tag="ACIE68_003183"
     CDS             complement(12363..13148)
                     /gene="ygcW"
                     /locus_tag="ACIE68_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFX5909457.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAASCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13467..14744
                     /gene="yqcE"
                     /locus_tag="ACIE68_003184"
                     /pseudo
     CDS             13467..14744
                     /gene="yqcE"
                     /locus_tag="ACIE68_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
     gene            14771..16249
                     /gene="ygcE"
                     /locus_tag="ACIE68_003185"
     CDS             14771..16249
                     /gene="ygcE"
                     /locus_tag="ACIE68_003185"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFX5909458.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNYFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         3885 a         4327 c         4153 g         4048 t
ORIGIN      
        1 caattcagta cgcggggttt tacccccgcg tggtggctac ttcttcgatt cgtaagcgag
       61 aactgcctta aactccatac cacgttctct gatactcaac tcacacaacg agtcacctac
      121 catcagcgtg aatcccagta ctgtacaaca cagtacgatg attgccacaa gggcatattt
      181 tgtcagcatg tcttgcctcc ctgcaaagaa gagactaaca ttcgaattgt ttgggttcga
      241 acgtaagcct caggttgata gaaatatcgc ctggggcttt tgtccatctg gaacctcgcg
      301 aatgcttaac gccagacagc ctcaagcacc cgacgccatt ctatacctga taattcttcc
      361 agcggcgttc acgtttaaaa tcaggaatac tgctcgcaaa ataaaaacgc ccccatcatt
      421 tctgacagag gcgtttaatt tatccggcaa tatttaccct tcgtgcaatc cacattccct
      481 ttttaagcca aagaaacgtg tttcttcttc caacatgcca ggttcccatt tacgggttgt
      541 atgggtatca ccgaccgata aatatccttc atcccataat gggtgatatt tcaggccatg
      601 tttttgcagg tactgataaa tagttcggtt atcccagtcg ataatcggca gcactttaaa
      661 tacgccacgc tgaattgcca gcaccggtaa attggcgcgg ctgccggatt gttcgcggcg
      721 caggccagca aaccacgttt gcgcattcag ttctttcagc gcccggttca tcggttcgac
      781 tttgttgatg tcattgtact tttcaatgcc ttcaacgccc tgttcccaca gtttgccgta
      841 gcgtgcttcc tgccaggcgg cgctttcggt agcgcggtac actttcaggt tgagcttgag
      901 tttgtccgtt aactcgtcaa taaagcggta ggtttccggg aacagatagc cggtgtcggt
      961 gaggatcacc ggaatatccg ggtgaatctg attcaccaga tgcaggctca ccgccgcctg
     1021 aatgccaaag ctggaagaaa gtacatattc accgggcaga ttatccagcg cccaggcaac
     1081 gcggccttca gcgtccagtt tttccagttc ggcgttagtt tctgccagcg ccagaatgcg
     1141 atctaccttc ggcagttcgt tcagggcgtt tagatcgagt ttggacatag attcctcact
     1201 gtttgccttg cctgatgcga cgctcacgcg tcttatcagg cctacaagac cgggctgatg
     1261 gttaatccca caaatcgcgc gccggatcga gcaccgggcg aatgatgccc gcacgcaccg
     1321 taaagtcgcc gaagccttca cccgcttcgc gctctttcgc ccagcgccct atcagttcat
     1381 caagcgacgc caggatttcc ggctcggtga tgttttcttt atacatccgt gggatacgtg
     1441 tcccaatgcg gttgccgcca agatgcagat tgtagcgacc cggtgcttta cccaccaggc
     1501 ccacttccgc cagcatcgcg cgaccacaac cgttcgggca gcctgttaca cgcatcacga
     1561 tatgctcatc gctgacacca tgtttcgcca ttaaattatc gatgttgtcg ataaaagacg
     1621 gcaggaaacg ctccgcttcc gccatcgcca gcgggcaagt cgggaatgac acgcaggcca
     1681 tcgagttttc acgctgcggc gtgacggcat tcattaaacc gctctctttg gcgatcttct
     1741 cgatcttcgc tttctcgctt tccggtacac cggcgatgat cagattctgg ttcgccgtaa
     1801 tgcggaaatc gcctttgtgg atcttcgcga tctccagcag gccggttttc agcggacgcc
     1861 ccggataatc aaggatgcga ccattttcga taaacagcgt caggtgccag ttatcatcaa
     1921 tgcccttaac ccagccaata cgatcgcctc gtccggtgaa ctcatatgga cggatcggtt
     1981 caaatttgat ccccgcgcga cgctccactt ccgctttaaa cgtctcaacc cccacgcgct
     2041 ccagcgtgta tttggttttg gcatttttac gatcggttcg gttaccccag tcacgctgag
     2101 ttgtcacgac ggcttcggcc accgccagcg tatgctccag cggcagatag ccaaactcgc
     2161 tcgccgtgcg ggcgtaggtt ttcttgttgc cgtgttcgat agaaagccca ccgcccacca
     2221 gcaggttgaa gcctaccagc ttgccgtttt cggcgattgc cacgaagttc atgtcgttgg
     2281 catgcagatc gatatcgttc tgcggcggga tcactaccgt ggttttgaac ttacgcggca
     2341 ggtaggtctg accaaggatc ggttcttcat cagtagtggc gactttttcc tggtcgagcc
     2401 agatctccgc atacgcgcgg gtacgaggca acagatgctc agaaatcttc ttcgcccact
     2461 cgtacgcttc cgcgtgcagc tgcgactcgt aagggttcga ggtgcagagt acgttacggt
     2521 tcatgtcgtt agctgtcgcc agcgcatcaa gaccgaccga gtgcagcatc tggtgcaccg
     2581 gtttgacgtt ctttttcaga atgccgtgga actgaaacgt ctggcggttg gtcaggcgaa
     2641 tgctgccata gatggtgttt tcaccggcaa atttgtcgat cgcctgccac tgtttagtgg
     2701 taatcacccc acccggcaga cgacagcgaa gcagcatcgc gtggcgcggc tccagcttct
     2761 gttcagcacg ttcggcgcgg atgtcgcggt catcctgctg atacatgccg tggaagcgga
     2821 tcagcaggaa gttatcgccc ttaaagccgc cggtcagacc gtcgtttaaa tcttccgcaa
     2881 tggtgccgcg caggtagttg ctttcaagct tcatgcgctc ggcgtctgtc agttttcctt
     2941 cgaccactaa aggccctgga tgtttttcgc tcattagtag acatctcgct gataacggcg
     3001 ctctacgcgc agctcactta aaaattcatc cgccgcttcg gtgtccatgc caccaaattc
     3061 ggcaatcact tccagaagtg cctgctcaac gtctttcgcc atgcgattag cgtcgccgca
     3121 gacataaatg tgggcaccat cattgatcca gcgccacagc tccgcgccct gttcgcgcag
     3181 tttgtcttgt acgtaaactt tttctttttg gtcgcgcgac caggcaagat cgatacgtgt
     3241 cagcacgcca tctttgacgt agcgctgcca ctccacctgg tacaggaagt cttccgtaaa
     3301 gtgcggatta ccaaagaaca gccagttttt acctggcgct tcgtcggcgg cgcgttgctg
     3361 cataaaggcg cggaacggcg caataccggt gcctgggcca atcataatca ccggggtttc
     3421 tggattggct ggtaggcgga agttatcgtt atgttcgata aatacgcgga cttcgccctc
     3481 ttcttccacg cggtcagcga ggaagctgga ggcaccaccg gcacgggcgc ggccttccac
     3541 gtcgtaacgc accacaccaa cggtgacgtg tacttcgttc tcgacttccg cctgcgagga
     3601 ggcaatggaa tagagacgcg gcgtcagcgg gcgcagcaga ttaattagcg cttcggcatc
     3661 aagctgtgcc ggggagaaac gcaccatgtc gacaatcggc gtcgtcgcgg cgtaatgctg
     3721 taacttcgct ttatcgccca ccagcggcag cagtgtttcg ctgcgggtaa gcgtggcgta
     3781 attctcaaca atgttggcgg tgttgacggt cagttcgaag tgccactgta gcgcttcgtt
     3841 cagaggcaac gttttgccct cgacggtgac aggttcatcg cctttcagcc acagcagttc
     3901 gacaagttct ttcaccagtg ccggatcgtt ctgataccag acgcccagcg cgtcacccgg
     3961 ctggtaacgc aggcccgagt cacctaagtc aatttcgata tggcgaacgt ctttttcaga
     4021 gttacgcccg gtaattttct ggttaacaga gaggctagcc accagcggcg cgtctttgct
     4081 gtacgggctg gtgtggattt catttaccgc gccagtagcg acggattgcg aaggtgccgc
     4141 gacaggcgca cgcgatttaa gcgcatcaac cacgcgggcg cgccactcgc tggcagcagc
     4201 ctggtattca acatcggcat cgacacggtc gagcaggcgt tcaccaccca gttccgccag
     4261 cttgctgtca aaatctttcc cggactggca gaaaaattca taagagctat cgccgaggct
     4321 aaacacggca aatgcggtgt tttccagctt cggcgctttt ttggagaaca ggaacttatg
     4381 cagcgcgacg gcttcttccg gcggttcccc ttctccttgc gttgacgtca ccacgataag
     4441 cagtttttcg ctggcgattt gtttgaattt atagtcgccc gcgttcacca gcttaacgtt
     4501 cagttttgcc gctaataaat catcacgtaa tgcttcagca acacggcgcg cattgccggt
     4561 ttgcgaggcg gagataatag ttatacccgg catttctgcg gcgggagctg gcgtcgctgc
     4621 aagcgcagca ggctgctgat tgagcacgcc ccagaaatag ccagaaaccc aggcaagctg
     4681 ggtgggagtt aaatcggtcg tggccgcctg aaggcgtacc agttgctccg ggttcaacgg
     4741 aagcaacgcg gaaggtggga cctgtgtcgt catgcgtcgt tatgttccag taagcaaagc
     4801 tgtttctgcg ccctgtcagc gcccataaaa cagaagagaa ggtaaggtta acggggtgaa
     4861 cggtgtggat taaagacggg atagcgataa ttaataacca aatcgactaa cctgttttag
     4921 caatagtctt taacaacaaa atagattaac caacctaatg aaaaacaaat gaatttagcc
     4981 aatcattaag ataaatcagc gattttgcgc aacaaagtcg ttttagataa tgcgaaaaaa
     5041 cagcctttcc ggtactctac ggcggtttta tcgtcctgta gagaaattat gatgtccacc
     5101 acgttattta aagatttcac cttcgaagcc gctcaccgct taccacacgt cccggaaggg
     5161 cataaatgtg gtcgcctgca cgggcattcc tttatggtgc gactggaaat taccggggaa
     5221 gtcgatccgc atacgggctg gattatcgat ttcgctgaac tgaaagcagc gtttaagcca
     5281 acttatgagc gcctcgatca ccattatctc aatgatattc caggcctgga aaacccgacc
     5341 agcgaagttt tagcaaaatg gatttgggat caggttaaac ccgttgtgcc gctgttaagt
     5401 gcggtgatgg taaaagaaac ctgcaccgct ggttgtatct atcgcggcga atgataagag
     5461 tgtgtcggcg gtcaatttcc cttaagtaac gctatgttag ggtgttgtgt tctggatatc
     5521 tggggcatga catggaagac gactgcgaca ttattattat tggtgccggt attgcaggca
     5581 ccgcttgcgc gttacgctgc gcgcgagcgg gtttatccgt tttgttactg gaacgcgctg
     5641 aaatccccgg cagcaaaaat ctttccggcg ggcggttata tacccatgca ctcgcggaac
     5701 tcctccctca gtttcatctg accgcgcctc ttgaacgatg catcactcac gaaagccttt
     5761 ccctgttaac gccggatggc gcaacgacgt tttccagctt acagcccggc ggtgaatcct
     5821 ggagtgtatt acgtgcacgg ttcgatccgt ggctggttgc cgaagccgaa aaagaaggtg
     5881 tcgaatgcat ccctggtgcg acggtggatg cactgtatga agaaaacggc agggtgtgtg
     5941 gtgtcatttg tggtgacgat attctccgcg cccgttatgt ggtgctggca gaaggtgcca
     6001 acagcgtcct ggctgagcgt cacgggttag tgactcgtcc tgctggcgaa gcgatggcgt
     6061 tggggatcaa agaagtgctg tcgctggaaa catccgctat tgaagaacgt tttcatctgg
     6121 agaataacga aggcgcagcg ttgctgttca gcggcgggat ctgtgatgac ttacccggcg
     6181 gcgcatttct ttatactaat caacaaacgc tctcgttagg gattgtttgc ccgctctctt
     6241 cccttacgca aagccgtgtt ccggcaagcg agctgctgac tcgctttaaa gcgcatccgg
     6301 cagtgcgccc gcttatcaaa aacacggaat cactggagta tggtgcgcat ctggtgccag
     6361 aaggtggctt gcacagtatg ccggtgcaat acgccggtaa cggctggctg ctggtgggcg
     6421 atgcgttgcg cagttgcgtc aataccggaa tttccgtgcg cggcatggat atggcgctga
     6481 ctggcgcgca ggcggcggca caaacgctga taagcgcctg ccagcaccgc gagccgcaaa
     6541 atctgtttcc gctttatcat cacaacgtag agcgcagcct gctgtgggat gttctacagc
     6601 gttatcagca tgttccggcg cttttgcaac gcccgggatg gtaccgtacg tggcctgcgt
     6661 taatgcagga tatttcccgc gatttatggg atcagggtga taaacctgtt ccaccgctgc
     6721 gccagttatt ctggcatcat ttacgtcgtc acggcctgtg gcatctggcg ggcgatgtta
     6781 tcaggagtct gcgatgtctg tagcccgtaa tctctggcgc gttgctgatg cgccgcacat
     6841 tgttccggct gactccgttg agcgccagac ggcagaacgg ttgattagcg cctgtccggc
     6901 aggtcttttt tcgctcacac cggaaggtga cttacgtatt gactatcgca gttgcctgga
     6961 gtgtggcacc tgccgtttgc tgtgcgacga atcaacacta caacagtggc gctatccgcc
     7021 ttccggattc ggcatcacct accgctttgg ataaataata aggataattt atgcccctct
     7081 tacacctgct ccgccagaat ccggtgattg ctgccgttaa agataatgcc agcctgcaac
     7141 tggcaatcga ttctgaatgc caatttattt ccgtgttgta cggcaatatc tgcaccatca
     7201 gtaatatcgt taaaaagatt aagaacgccg ggaaatatgc ttttattcat gttgatttgc
     7261 tggaaggcgc gtcaaataaa gaagtcgtta ttcagttttt gaaactggtg accgaggcgg
     7321 acggcattat cagtaccaaa gcctcaatgt tgaaagcggc aagagcggaa ggttttttct
     7381 gtattcatcg cctgtttatt gttgattcaa tttcgtttca caacattgat aagcaagttg
     7441 cgcaatcgaa tccggattgt attgagatcc tgccaggctg tatgcccaaa gtgctgggct
     7501 gggtgacaga gaaaatccgc caaccgctga ttgccggtgg gctggtgtgc gatgaagaag
     7561 atgcgcgtaa tgcgattaac gcgggtgtcg tggcgctttc caccacgaat accggggtct
     7621 ggacgttagc gaaaaaatta ctttgacggg ataatcgtaa ccaattgaat ttggtttgat
     7681 ttgtaggccg cacgccacat ccgacattca gcgcctgatg cgacgcttga cgcgtcttat
     7741 caggcctaca agtccccagg ccagaaccgt aggcaggatc atccggcaac acgttattgg
     7801 caatctgcgt gaatgtttgt caccagtgcc tccagcacca ctttccagtc atcaaccacg
     7861 ccaacatctg cctgtgaaaa taccgcagcg ctggcgtcat gattaatcgc caccacaaac
     7921 ttgctattgc gtacgcccgc cattaacgct gctgcgccag aagcgccaac aacaatacac
     7981 accttaggtg ccagcagatg tccggaaata ccaatcactt tttcagcatc aacgccaccg
     8041 ttcatcactc gcgcccgact gtagcccact tcggctccca gcttttctgc cagcatagcg
     8101 atttcctggt tatctgcctc tccgccctgc cccaccacca gcacccgtct ggcctcagct
     8161 aatgggtcgc gagtaacatt tttcagatcc tctgtgctaa ccagccagtc cggaagcgca
     8221 cccgggacaa tgatcagttg ttgcattcca gagggcaacg tggcgttttt cgctgctccc
     8281 gcctgacgcg ccagggaaag gcatagcggg cgtttttctg tttgcagcgt cgccgttagc
     8341 gcatttcccc agtgggattt gcgcacgctt acggttggaa tatctaacga agtcacctgg
     8401 cagatgcttg caccatgtaa acgccaggcc agccgggtcg acagttcatc gccaaacgtt
     8461 cccggcggaa agagaacaac gtccgcaggc gttcgctgcc actgttccac caacgcatcc
     8521 agcacctgtt ccgccactac aggctgaggt tcaatctgcc agtgcgccag ggtacaaccg
     8581 ctgaaatcct gcgccgccag ccagcttgct attgcggcat tctcctgatt tattgtcaca
     8641 attgcgatat tcatggctgc atcctttggc gtaaatagtc ccgccatagc ttttgagctt
     8701 tttctgctac cgtttgccca tcaatcagcg tcgctccgcg tcgttgctct gctcttgcca
     8761 gttgcagaca ctgcatcgct ggcatttcgg cagcaacagt tttacggata atttctgctt
     8821 tccccgcagc cattcgctgg cgcattcccg gtactggcaa ggccacttca ccgcactgac
     8881 gcacggcaat caccgcaggc aagcgaaccc ggcaacaacg caacccatgt tcagtacgct
     8941 gttcgagggt gataaacagc gcgtcgagag tgaaacgctc cacctgggtg aagcagggcc
     9001 agcccagcat ttccgccagc aaaaatggcg tttgcccatt ctgcccttcg ctgctttggc
     9061 agccggtaat tatcagatcc agcggattct gatgctgcca ttcggcaata tggcgagcga
     9121 caaattccgg cgcaaagcgc agatctgccg ccgtctccag caatacagct tcctcaaacc
     9181 caagagccat gagatagcgc agccagtgca atgcccgttc atcccccatg ctcaacgcgg
     9241 ttaaagacat cggcgtaccg ttttttctct gcgccaacag cagcgcggca gcggcctgtt
     9301 catcagcacc gagtaaactt cgcagtagcg aaatatccgg tccgctttta ccctgagccg
     9361 ccgcctgcca ctctttttcc gccagcattc cggcatccgg ttcggcttta aacgctaaca
     9421 gaatgttcat tcgcacctcc tacatttccc gccgccacca gcgggagggc tttagtttct
     9481 ggtgcccata gccaggtcac aacaaaacca accaacaaca ctgtcgccag gagcaataag
     9541 gtgacttgca ttccccactg cgccagcacc cacggcagca ggccagtact taccgccgcg
     9601 ccaagtcgac tcatggcggt ggcaaaaccg acgcccagcg agcgaatgtc agtaggaaaa
     9661 ctttccgcag gcaaaatgcc caccagatta ctgactgccg aaatggtggt gctgaagaga
     9721 acaaaaagca gcagcgttaa tgaactgccg gaaggcaaac aggccatgac taccagcgtt
     9781 gccgccagca gcaaaaaact tcccagcaaa aatttgcgat gtgccagcag gtgcgtcaga
     9841 actaatccca gcagcgcgcc cacaattaac aacgcattaa gcatcaggct ggcagtcagc
     9901 gcatcttcca gaccaatagt ctgggcgata gttggcagcc aggtataaat cacaaaccat
     9961 gggattacga ggcagacaaa gaagacgctg ttaaacgccg tgcgccgcca gtaacgcgaa
    10021 gagaacaagg ttttgatgtg tttatgggtc gccgttacca cttcatcgcc cagtaaaaca
    10081 tggggaccaa aatagcgatg cacgatagcg tgagcttctg caaaacgccc ctggcgcagt
    10141 agccagcgtg gtgattctgg cgttccccag cgtaataacg taatcaacaa cgcgggcaga
    10201 gctgccgatg ccagtagcca gcgccaggcc tccgggtttt cggaaataaa gtgatgtccg
    10261 gcaatacttg ccagcacata gcctacggtc cacaccacgc tgaatgcgcc cagcaaaata
    10321 ccgcgatggc ggcgcgggga aaattcagcc agcaaggtgt gacctactga ataatcgcct
    10381 cccagaccaa tgccaatcaa aatgcgcagt ccaataagat gctctggcgt ggtggcaaaa
    10441 aattgtaaaa acgaagcaag cgtaatcagc aaaaagctga aggtgaagat tttttgccga
    10501 ccaatatggt cggagatcca cccaagaacc aggctgccaa ggaacaaacc aaggagcgcc
    10561 gagccgccga tcattcccgc cataaacggc gtcagttgca tggcgggcgt aagctgaata
    10621 atggcgtaac caatgacgcc gagaacataa ccgtcggtca ggtgtgcgcc gaaagtgagc
    10681 gcagcaatgc ggcagtgaaa acggttaagc ggtaaatcat ccattcgcac cggtgaagtg
    10741 ttcatcttta ccccttcatt gtccggtaat aagaacggct tctcgcctga gaagccgaaa
    10801 aggcaacgcc ccgtcatcgc ggaacgctgc ctgacacatt atttttcaat cggatagata
    10861 gtacccgtat tcataatgcc attaggatcg aactgctttt tcagcccttc cagcaacgcc
    10921 cacgcgctgc cgtgttccag tttgctccag tgaacgcgat gtttaccgat accgtggtgg
    10981 tgcaccatcg aaccgccgag gcgaatggtt tcttcacaga tgatcttgtt gagcggattg
    11041 tggtacttgt cgatttcctc ttccggctta cagtcaacga cgttgtaatc gtagacgaag
    11101 tacatgttgg tgccgttctg atagctatga gaggaatgac cgcccagcat ggtgatgtcg
    11161 tcggcgtgcg ggaactcagt acgaatacgg ttaataacgc tttcgtagat ttcgtggatg
    11221 cagctccagc agccggacac ttcagtggta aagcccatgt tgccggtttt gaggatctgc
    11281 acacgttcgg cagccacttt atccggtccc cagttcaggt tgttaaacca ggtttcgatc
    11341 agtttgctgt ccacgcgctg gcactgcggg tagcgggcaa cgatttccgc aatcccttcg
    11401 cccgtcgcct tcgcaatgcg aggattgcct tcagccataa agatcagcac acatttaccg
    11461 tcggcaaaat gggtgaagtg ttgggtgcca tcttcagcgt catacaaacg agcgatcgac
    11521 ggacgatacc cttccaccat gacttcacgc aggatgttga agccggtttt catgtcttcc
    11581 aggatatagc cgtagaagag gttgttttcc ggggtgaatt taaagatttt cactgttact
    11641 tcagtgatat agcaaagtgc accttcgtta ccgatgatga tgtggcgaat gtccgggcca
    11701 gccgcgcggc gtggcacgtt tttaatgcgt gtgacggtgc catcagccaa tactgcttcc
    11761 agaccaacga ccatatcttc gattgcgccg tagagtgtgg agaactgccc gatactgcgg
    11821 gttgctacca ggccgcccat ctgcgccagc ggctttgact gcggagaatg ccccgtggtg
    11881 taaccttttt cacgcaacgc attttccagc acctccagcg gaacgccaca ttgcgccgtc
    11941 gcctgcatat tctcaatatc aatattaatg atttgattca tggcggagcc gtcgagcacc
    12001 accgagtttt ccacaacggt ttccagccca ccttcagtgg cggaagcgcc ggtacgcggc
    12061 acaccgttaa ttttgtgcgc attcataaaa ttcagcacac gggagacttg ttctgtggaa
    12121 ccgagtttta cgaccgctgc cggaatcggc aaagtataaa taccatgaat atccggaaat
    12181 ttacgaaaac ggtcaatact gttcttcttt aatactgttt catcggtaat tacgcgatct
    12241 gcaccaacaa tttcctttag ttggtcgaca atcgctgcgc gagataaaga catagtaatt
    12301 ccttcctgat aataaaaatg gaataatcat taataagaga aaacagaaac caccgttatg
    12361 ggttagcgca ctaaataacc gccatcaacc actaataaat gcccgttgac ataattcgat
    12421 gccggacttg cgaggaatac ggctgcgccc attaaatcct gagtatcgcc ccaacggttt
    12481 gccggaatat gatcaagaac gcgctgattg gtttctggat tactgcgtgt cgccagcgta
    12541 atatctgttg cataatagcc aggggcgata ccatttacct gaatattata ttgacctagt
    12601 tcatcacaat aagctttggt gaacccggca agagcatgtt tagtggcaga atatgcaggt
    12661 gaccattgtc cacctaagta agagaacaat gaacagatat taataatttt gccgcttttc
    12721 tgcgggatca taatttttgc agcttcatag cttaactcga atgcggcggt caggttcaca
    12781 tcaatcatcg gatcccagtc ggcacgaccg aagtccagca ccttattcag cttacaaata
    12841 ccggcattgt taaccagaat atcaactgta ccgaaacgct cacagctagc agcgataatc
    12901 ttctgcggcg cgccttctgc ggtgatatcc acctgcatga agtccacctc aacaccctgt
    12961 ttttcaatca tttcctttgt ttcgccgtta tctttgacga aactaggaat aaagatattt
    13021 gcgccagctt tggccaacgc catggcaaat gcctggccta aaccgctatt cccaccggta
    13081 acaattgcgg ttttaccttt cagggagaaa aaatccattg agaacgcatt gagagattcg
    13141 attgacatag ttagctccaa tttccgtaag gcaaaaaaaa agaaaggtaa cttcccccgc
    13201 gagagggaag ttacctttct cattatctct ggtaactgta gataattttt agcataacgg
    13261 cataactgct aatgtgatcg taatcacagt gtgatattcg tattgtcaaa tagttttaaa
    13321 aagcagaagc gtgattactc tcacacaaca cctctgcttt ccatgttatt tcttattgca
    13381 attactagag acctgagaaa agtaatccac ctgaaagggc ggcttacttt tctctttttt
    13441 ttgtctatta caaatgggaa tacgatatgc aacacaactc atatcgccgt tggataaccc
    13501 tcgcgataat tagttttagc ggcggcgtta gtttcgacct ggcttattta cgttatattt
    13561 atcaaattcc catggcgaaa tttatgggat tcagcaatac cgagataggt ttaataatga
    13621 gtacctttgg tattgcggct attattcttt atgcccccag cggcgttatt gccgataaat
    13681 tttcacaccg caaaatgatt acttccgcga tgatcattac cggattactg ggtctgttaa
    13741 tggcaacgta tccaccgctc tgggtaatgc tctgtattca ggtcgccttt gcgataacaa
    13801 cgattttaat gctgtggtcg gtgtcgatta aagccgcctc gttgctgggc gatcatagcg
    13861 agcaagggaa aattatgggc tggatggaag ggctgcgcgg cgtcggtgta atgtcgctgg
    13921 cggtgtttac catgtgggtc ttttctcgct ttgcaccgga tgacagcacc agcctgaaaa
    13981 cggtcattat catctatagt gtggtttaca tcttgttggg gattctgtgc tggttttttg
    14041 ttagcgataa caacaacctg cgcagtgcca ataacgaaga aaaacagtca ttccagctta
    14101 gcgacatcct ggccgttttg cgcatcagca ccacctagta ttgcagcatg gtgatttttg
    14161 gtgtcttcac catctacgcc attctgagtt actccaccaa ctatctgacc gaaatgtacg
    14221 gcatgtcgct ggtggcggcg agctacatgg ggattgtgat caacaaaatc ttccgcgcgc
    14281 tgtgcggccc acttggcggc attatcacca cctacagtaa agtgaaatcc cctacccgcg
    14341 tgattcaaat cctttccatc atcggtttgc tggcgttgac agccctgctc gtcacgaact
    14401 ctaacccgca atcggtcgcg atggggattg gcctgatttt actgctggga ttcacctgtt
    14461 acgcctcacg cgggctgtac tgggcctgcc ccggcgaagc gagaacaccg tcttacatta
    14521 tgggcaccac ggtaggtatt tgttcggtga ttggattcct gccggatgtc ttcgtttacc
    14581 cgattatcgg ccactggcaa gacaccctgc ccgctgcaga agcctaccgc aatatgtggc
    14641 tgatgggcat ggcggcgctc ggcatggtga ttgtctttac ctttttgctg ttccaaaaaa
    14701 ttcgtactgc tgatagcgcc cccgcaatgg ctagcagcaa gtaagccagg aaaattacag
    14761 gagaaaaatc atgtcgaaaa aatacatcat agggattgat ggcggaagtc agagcacaaa
    14821 agtggtgatg tacgatctgg aaggtaatgt ggtttgcgaa ggtaaaggct tattacagcc
    14881 gatgcacacg ccagatgccg atactgcaga acatcctgac gacgatttat gggcatcatt
    14941 atgttttgcc ggtcacgatt tgatgagtca gtttgccggg aataaagaag atattgtcgg
    15001 tattggtctg ggatccatcc gttgctgccg tgcgttattg aaagccgatg gcacgccagc
    15061 tgcgccgttg attagctggc aggatgcacg cgttacacgc ccttacgaac atacgaatcc
    15121 tgacgtggcg tatgtcacct ctttttcggg ttatctgacg catcgcttaa ccggcgagtt
    15181 taaagacaat atcgccaact attttggtca gtggccggtg gattataaga gctgggcatg
    15241 gagcgaagat gctgcggtaa tggataagtt taatatcccc cgtcatatgc tgtttgatgt
    15301 gcaaatgcct ggcaccgtcc tcggacatat cacaccacaa gccgcactgg cgacacattt
    15361 cccggcagga ctgccggttg tttgtaccac cagtgataaa ccggtagaag ctctgggggc
    15421 cggattactg gatgatgaaa ctgcggtaat ttctcttggc acttacatcg cattgatgat
    15481 gaacggcaaa gcactgccga aagatccggt ggcgtactgg ccgattatgt cttctattcc
    15541 gcaaacattg ctgtatgaag gttacggtat tcgcaaaggt atgtggacgg tgagctggct
    15601 acgcgacatg ttaggcgagt cgttaattca ggatgccaaa gcacaggatc tttcaccgga
    15661 agatttgctc aacaaaaaag cttcttgtgt gccgcctggc tgtaatggtc tgatgacggt
    15721 gctggactgg ctgaccaacc cgtgggaacc ctacaaacgt gggattatga tcggctttga
    15781 ttccagcatg gattacgcat ggatatatcg ttcgatactg gaaagcgtgg cgctgacgct
    15841 gaagaacaat tacgacaata tgtgtaatga aatgaattac tttgcaaagc atgtgatcat
    15901 tactggcggc ggttcgaaca gcgatctgtt tatgcagatt tttgccgacg tgttcaacct
    15961 tccggcacga cgtaacgcca ttaacggttg tgcaagcctg ggggcagcga ttaatacagc
    16021 ggtaggtctg gggctatacc cggattacgc aacggctgtc gataaaatgg ttcgcgtgaa
    16081 agatatcttt atgcctgttg agagcaatgc caaacgctac gacgcgatga ataaaggcat
    16141 tttcaaagat ttaaccaaac acactgatgt gattctgaaa aaatcgtatg aagtgatgca
    16201 tggggaattg gggaatgcgg attcgatcca gagctggtcg aatgcgtgaa tgaggtaggc
    16261 caggcggaaa actgcccggc ctgaacatac ctaaatagtt atccccgctg acgcggggaa
    16321 cataagttcg acaacttaaa aaatgataac aagtccggtt tatccccgct gatgctggga
    16381 acataataaa aactttaact agttcggttt atc
//
LOCUS       DBANUU010000094        16309 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000094 DBANUU010000000
VERSION     DBANUU010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.094"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(523..948)
                     /gene="arsC"
                     /locus_tag="ACIE68_003186"
     CDS             complement(523..948)
                     /gene="arsC"
                     /locus_tag="ACIE68_003186"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFX5909459.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGITVRALLRKNVEPYEELGLAEDKFTDERLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(961..2250)
                     /gene="arsB"
                     /locus_tag="ACIE68_003187"
     CDS             complement(961..2250)
                     /gene="arsB"
                     /locus_tag="ACIE68_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFX5909460.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLVALALRLSFTL"
     gene            complement(2304..2657)
                     /gene="arsR"
                     /locus_tag="ACIE68_003188"
     CDS             complement(2304..2657)
                     /gene="arsR"
                     /locus_tag="ACIE68_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFX5909461.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            3397..3480
                     /gene="dinQ"
                     /locus_tag="ACIE68_003189"
     CDS             3397..3480
                     /gene="dinQ"
                     /locus_tag="ACIE68_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFX5909462.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3534..4886)
                     /gene="gorA"
                     /locus_tag="ACIE68_003190"
     CDS             complement(3534..4886)
                     /gene="gorA"
                     /locus_tag="ACIE68_003190"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFX5909463.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4958..5800)
                     /gene="rlmJ"
                     /locus_tag="ACIE68_003191"
     CDS             complement(4958..5800)
                     /gene="rlmJ"
                     /locus_tag="ACIE68_003191"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFX5909464.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6003..8045
                     /gene="prlC"
                     /locus_tag="ACIE68_003192"
     CDS             6003..8045
                     /gene="prlC"
                     /locus_tag="ACIE68_003192"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFX5909465.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8053..8805
                     /gene="rsmJ"
                     /locus_tag="ACIE68_003193"
     CDS             8053..8805
                     /gene="rsmJ"
                     /locus_tag="ACIE68_003193"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFX5909466.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8854..10323)
                     /gene="dtpB"
                     /locus_tag="ACIE68_003194"
     CDS             complement(8854..10323)
                     /gene="dtpB"
                     /locus_tag="ACIE68_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFX5909467.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            10489..10542
                     /locus_tag="ACIE68_003195"
     CDS             10489..10542
                     /locus_tag="ACIE68_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909468.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(10641..11075)
                     /gene="uspA"
                     /locus_tag="ACIE68_003196"
     CDS             complement(10641..11075)
                     /gene="uspA"
                     /locus_tag="ACIE68_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFX5909469.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11466..11801
                     /gene="uspB"
                     /locus_tag="ACIE68_003197"
     CDS             11466..11801
                     /gene="uspB"
                     /locus_tag="ACIE68_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFX5909470.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11872..13371)
                     /gene="pitA"
                     /locus_tag="ACIE68_003198"
     CDS             complement(11872..13371)
                     /gene="pitA"
                     /locus_tag="ACIE68_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFX5909471.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            13603..14805
                     /gene="yhiN"
                     /locus_tag="ACIE68_003199"
     CDS             13603..14805
                     /gene="yhiN"
                     /locus_tag="ACIE68_003199"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFX5909472.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15120..16172)
                     /gene="yhiM"
                     /locus_tag="ACIE68_003200"
     CDS             complement(15120..16172)
                     /gene="yhiM"
                     /locus_tag="ACIE68_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFX5909473.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4201 a         4122 c         4218 g         3768 t
ORIGIN      
        1 tcttcctgat ataattacct ttgacttccc ttttatctcc acccggtaat atttgtgtat
       61 aaaaaagggc attgatagtg tcgtaaaaga aaacgttaac aactatcaaa aagcgctcat
      121 tttaaaaagt tatgatgttc tccgatagtg tgtgaaatga tatttttgtg atgttttaga
      181 tgtgtaataa taaaatgggt taaggtatct tataattatt taataatttg ttgtgtaaag
      241 cactgataaa tcataatcca gtacgtcttt aaactgctgc actgtatcgc tgatcactaa
      301 gcagatcact gatacaccat gctggacgcg ataattggaa tctcacgcag ggagtaatgt
      361 gtaaatagca aaatcgcctg atgcgctacg cctgtcaggt ctacaaataa catacaattt
      421 attgaatttt catgactttg tagatcggat aagacgcgtt agcgtcgcat ccgacataaa
      481 caaagcgcac tttaccaact atcggcagtg gatatcgccg ctttatttca accgcttacc
      541 cgcttcatca accactttct cgccatcttc cttggagaac gcgccttttt gcgcatctgg
      601 cagaatttcc agcaccactt ccgaggggcg gcataggcga gttcccagcg gcgtcaccac
      661 aatcggacgg ttaatcagaa tcgggtgctg aagcataaag tcgattaacc gctcgtcagt
      721 aaatttatct tccgcaaggc ccagttcctc atacggctcg acgtttttac gcagcagcgc
      781 gcgtacggta atccccatat cggcaatgag ttttaccagt tcatcgcgcg ttggtggtgt
      841 ttccagataa tggataatgg tcggttctgt accgctgttg cggatcatct ccagcgtatt
      901 acgcgatgtg ccgcaggccg ggttgtgata aatggtaatg ttgctcatat cagtatctca
      961 ttacaaagtg aaagagagac gaagcgccag cgcgacgagt gtcacaaaca gcacaggcag
     1021 ggtcatgata atccctgtac ggaagtaata tccccagctg atagtcatat tcttctgcga
     1081 aagtacgtgc agccagagca gcgttgccag gctaccaatc ggggtaattt tcggtcccaa
     1141 atcgcagcca atcacattgg cataaaccat cgcttctttg ataacgccag atgccgtgct
     1201 gccatcaatg gacaacgcgc caaccagtac cgtcggcata ttgttcataa tagaagagag
     1261 gaaggcggtg aggaatccgg tgccgagcgt cgcggcccac aggccgttat ccgccagcac
     1321 gttgagtacg ccagaaagat attccgttaa tccggcattg cgcaggccat aaaccaccag
     1381 atacatgccg agcgagaaga tgacaatctg ccagggggca ccgcgcagga ctttacccgt
     1441 attaatcgca tgaccgcgtt tagcgacgac aaataatatc agcgcgccca cagctgcaat
     1501 ggcgctcacc ggaatgccga gcggttccag gacgaaaaat cccaccagca gaagcagtaa
     1561 aacaacccag ccagttttga acgtagcagg atctttgatc gcttctgcgg gagatttcag
     1621 cagcgccata tcgtagttct gcggaatatc tttgcgaaaa tagagatgta acatcaccag
     1681 cgtggcaaca atcgcggcga tatccaccgg caccatcacc gaggcgtatt cgcgaaagcc
     1741 gaggccaaag aaatcagcgg aaacgatatt caccaggttg gagacaataa gcggcaggct
     1801 ggcggtatcg gcaatgaatc cggccgccat cacgaacgcc agcgtagtgc ctttactgaa
     1861 ccctaaagcc agcagcatgg cgatgacaat cggtgtcaaa ataagcgccg cgccatcatt
     1921 ggcaaacagg gcggcaacgg cagcaccgag caggacaatc caggtaaaca gcaagcgacc
     1981 acgaccatta ccccagcgtg agacgtgcag cgccgcccat tcaaaaaagc cggactcatc
     2041 cagcagcagg ctgatgataa tgacggcgat aaacgcagcc gtcgcgttcc agacgatatt
     2101 ccacaccacc ggaatatcac ccggatggac cacgcccgta actaacgcca gtactgcgcc
     2161 gagcgttgca ctccagccga tgcctaaacc tttcggctgc cagataacca atacgatggt
     2221 caggacaaag atagcgcctg ccagtaacat aatgcctccc ggataaaaca catctgaaaa
     2281 ttcatatgtg tttagctaaa tttttaactg caaatgttct tactgtcccc ggaacagttt
     2341 tgtcgagcca ggttgcggac aatcgcctga accttttcct gttcacatcg ccaggcctca
     2401 tcaataattt tcgccgccca tgctggaata tgcggtgata agcggtaatg aacccactta
     2461 ccttgcttgc ggtccagcaa tagcccgctt tcacgcagca atgccaggtg gcgggagatc
     2521 ttgggctgcg actggtcgag agcagtgcag agatcgcaga cgcataactc tcccagttcg
     2581 ctgagcagta aaacgatgcc cagacgggtt tcatcagcaa gaattttgaa caattggatg
     2641 ggtaacagaa atgacatatt gcgctcctga ttgttgcagg tagtgtctct cttcgaagcg
     2701 gataagtcaa aaacatatat gacttaacga atgtgtaagt gcagaggaag gtaataggtg
     2761 tgaattttga gttggctatt catttgaaag gaggtctgaa tcaggacgta aaaaaaccga
     2821 ctttgcgtcg gttttttact ttccagccct gagttggtgg ctctggctgg agtgagagag
     2881 ttcttatcta acagctcaat actattagcc agtggctaac attgcaagaa ttagcgattt
     2941 cttcagctgg cggagggggc acttcggtat atatcgcaat gacgatttat atactttcac
     3001 tgggtcatcg tcatattaag ccttgtccga atccggatgt aaaaaaaccg actttgcgtc
     3061 ggttttttac tttccagccc tgagttggtg gcactggctg gagtgagaat gccattatct
     3121 aacgcatcaa tgctaatacc atactgaaac tattgcaagg acatgctggt tttataacct
     3181 gaatgtactg tatgattatc cagttagctc tgaggcattt tcactctggt aatgcgcata
     3241 aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg
     3301 agtgagaatg ccattatcta acaatagggc atgcgccgtg acaggcagtg gatgagtaag
     3361 cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt
     3421 cttggggcgt taattgcgct gctggaactg atccgctttc tgcttcagct tctgaactga
     3481 tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc
     3541 attgtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt
     3601 ttggttgcac ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag
     3661 ccaatgccgt gaataccgac aatcttctct tccgatccaa cgcacaccag cttcatgcgg
     3721 cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact
     3781 ttcacctgat cgtcgccata ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca
     3841 atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc
     3901 ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagc
     3961 tccactgcac ccgtgttatc gcccaccgcg taaatacctt caatattggt gttttgatat
     4021 ttatcgacga cgatatagcc tttttcgtta gttttaacgc cagcggcttc caggttgatg
     4081 ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga
     4141 ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg
     4201 gcgttggtgt gcagctgcgg gccttcggcg ttcatcactt cgaccagcgt ttcggaaatc
     4261 atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg
     4321 aggccgttaa tcacgcccgc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg
     4381 cgctctggca aagcaggaag ggcgaagaag ccatcagaat caataccgta ttccacgccc
     4441 ggaatatccg ggtggctcgg acgaccgcct gtggcgatca gaatatgatc ggccgtgatg
     4501 gtttcgccgt ttacctccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca
     4561 tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg
     4621 gtacggctgg cgatcaacgt ttcccagttg aatttattga tagtggtatc aaaaccataa
     4681 tccgggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc
     4741 ggcacacagc caacatttac gcaggtgccg cccagctctt tggcttcaat cagcgcacat
     4801 ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg
     4861 gcgatgtaat catagtgttt agtcatgaca aagtgtcctt atcgttgatt accgcgattg
     4921 tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta
     4981 cggtggcgtg cccggtgcct gccggaacca gtttgctgtg cagccacggc agcacgttat
     5041 tcatctgctg ttccagtttc cacggcgggt taatcacaat catgccggaa gcggtcatgc
     5101 cacggcgatc gctgtctggc agtaccgcca gttcaatttg cagaattttg cgaataccgg
     5161 tcgcttccag atcgtggatc atgcgcttaa tttgctgacg cagcaccacc ggataccaca
     5221 gtgcgtaagt accagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct
     5281 gatagtcggt tttcatttca tacggcgggt cgataaggat taaaccacgg cgggaaaccg
     5341 gcggcagttt ggccttaagc tgctggaaac catcggcttt ttcgacgcgc gcacggctgt
     5401 ctttctgaaa ttcagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     5461 ggctgtcttg ttcgcgcagt agctggcgag caatcaatgg cgaaccgggg tagtaacgca
     5521 actgaccgct acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca
     5581 aatcgtcctg ctgccagata cgggcgatgc cttcgagata ttcgccggta cgctcggcat
     5641 gttcgctgcc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     5701 tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg
     5761 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     5821 gtaaaaatcg gcccgtattc ggggcgcaca aaagcttatc aggacagtat accgaaaaga
     5881 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     5941 acacacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     6001 gaatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     6061 aacatgtcgt tccagccgtg actaaggcgc tgaacgactg ccgcgaaaat gtggagcgcg
     6121 tagtagcgca aggagcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg
     6181 acgatgtgct ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc
     6241 cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac agcacctggg
     6301 tagggcaaca tgaagggctg tataaagcgt atcgcgacct gcgcgatggc gatcattacg
     6361 ccacgctgaa cacggctcag aaaaaagcgg ttgataacgc actgcgcgac ttcgaactct
     6421 ctggcatcgg tctgccaata gagaaacaac aacgctacgg tgagattgcc actcgtcttt
     6481 ccgagctggg caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac
     6541 tcgttaccga cgaagcggag ctggcgggga tgcctgaaag cgcgctggct gcggcaaaag
     6601 cccaggccga agcgaaagag ctggaaggct acctgctgac gctggatatc ccaagctacc
     6661 tgccggtaat gacctactgc gacaaccagg ccttgcgtga agagatgtat cgcgcttaca
     6721 gcacccgcgc ttccgatcaa ggcccgaacg ccggtaagtg ggataacagc aaggtgatgg
     6781 aagagatcct cgctctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct
     6841 ttaaatccct tgctactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg
     6901 atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct ggcacaactg cgcgccttcg
     6961 ccaaagcgga atttggcgtc gatgagttgc agccgtggga tatcgcgtac tacagcgaaa
     7021 aacaaaaaca gcacctctac agcatcagtg acgaacagct gcgtccgtac ttcccggaaa
     7081 acaaagcggt taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag
     7141 agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa
     7201 ataacgaact gcgcggcagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg
     7261 gggcgtggat ggatgactgc gtaggccaga tgcgtaaagc tgatggttcg ctgcaaaaac
     7321 cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca
     7381 ctcacgacga agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga
     7441 cccgcatcga aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac
     7501 tgccgagtca gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctccg
     7561 gtcactatga aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga
     7621 agaactacca ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc
     7681 gccttcatgc tgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa
     7741 tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca
     7801 gccatatttt cgccggtggt tatgcggcag gttactacag ctacctgtgg gctgacgtac
     7861 tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc
     7921 agtcgttcct cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca
     7981 aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg
     8041 gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta
     8101 tctgttctgg cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg
     8161 ttaacgccgg aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatattt
     8221 gttgattttg ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag
     8281 gcggtggcga aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc
     8341 gcagggctgg ggcgcgatgc ctttgtgctg gcttcagtcg gttgccgcgt gcggatgctg
     8401 gagcgtaatc cagtcgttgc cgcattgctc gacgacggcc tggcgcgtgg ttatgcggat
     8461 gcggaaatcg gcggctggtt gcaggagcgt ttgcagttaa ttcacgcctc cagcctgacg
     8521 gcgctaaccg atattacccc acgcccgcag gtggtctatc tcgacccgat gttcccgcat
     8581 aagcagaaaa gcgcgctggt gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg
     8641 gatcttgatg ccgatggatt gctggagcct gcacgcttgc tggcgaccaa acgcgtggta
     8701 gtaaagcgcc cggactacgc gccaccgcta gcgaatgtcg ccacgccaaa cgcggttgtg
     8761 actaaagggc atcgctttga tatctatgca ggtacgcccg tgtaaaagta aagcagggta
     8821 gcgataacgc taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat
     8881 gcgtttcagc cacggcacca tcaacagcat cactactgca acgcccagtg tgaccagacc
     8941 aatcttacca aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc
     9001 cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag
     9061 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
     9121 caggccaagg gcgctaataa acagttcacc taagctctgg aataagtaca ccagcacgat
     9181 aaaccatggc gatgtcagcc cctgggcatc cgcaaaccac attcccgcag ctgcagccgt
     9241 caaaaagccc agagagcaca taaacatgcc gagagtaaat ttcatcggca tcgagaggtc
     9301 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
     9361 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
     9421 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
     9481 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
     9541 atccagcttg aatgcctgac gaaagaagat gatggtgacg acgatggaga gaacaatcag
     9601 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
     9661 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
     9721 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
     9781 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
     9841 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
     9901 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
     9961 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
    10021 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccgagaa caatggtgcg
    10081 tttggtcccc agcaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
    10141 cgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
    10201 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
    10261 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
    10321 catatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
    10381 gaaaaagttt acatctgtca gagattctta atattgatgc ggtgaataga ttaatacact
    10441 attacaacag aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat
    10501 gttgtattgg caggagattt tgattgcgta cgcagatcgt aggccagata aggggtttac
    10561 gctgcatcag gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg
    10621 aacacgtcgt ggggggatga ttattcttct tcgtcgcgca gcggaacaat cagcatatca
    10681 acgtgaacgg tgttgatcag ctgacgtgcg gaagacatta gtttgctcca gaagtcctgg
    10741 tggtgaccac aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacctgg
    10801 cccaggtcgc cgctgccgct cagggtttca gtgattgggt agcctgcatt agtggaaagc
    10861 tcggtcagcg catgatgtgt ctcttcagag atgcgtttct gcatatcacc cagattcaca
    10921 tcaataagcc cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc
    10981 gcattgtagg ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag
    11041 aggtcgaccg cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt
    11101 cgatgactgg ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg
    11161 tgcgcggggc taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga
    11221 cccggatgat ccgtcaatcc gccttgctta ctcgttagat aaacaaattt tcgacatgaa
    11281 tatattgata gtggttaacc ttctggaaaa aaaacaacct gatctcctac actatctata
    11341 gagccgctcg gatgttggtc accacaaaag cggtttctcc aggacctggt gttaactgat
    11401 tggcagcgta tcgggagcgg tagccccggg gtggaactcc gcgggcaggt cgccggggag
    11461 gaggtatgat aagcaccgtc gcattatttt gggctttatg tgtcgtttgc attgttaaca
    11521 tggcgcgcta tttctcatca ctacgcgcgt tgttagtggt actgcgtaac tgcgatccat
    11581 tgctctatca atatgttgat ggagggggct tttttacctc acatggccaa cccaacaaac
    11641 aggtgcgtct cgtttggtat atctatgccc aacgttatcg cgatcatcac gatgatgagt
    11701 ttattcgccg ctgtgagcgg gtgcgtcggc agtttattct gactagcgca ttgtgtggtc
    11761 tggtggtggt cagcctgatt gcattgatga tttggcatta agcacttcag caataataac
    11821 gcataaaaaa agcgggccag taaactgacc cgctcgtttt ggtgcgtacg attacaggaa
    11881 ctgcaaggag agccagtaca gcccgccgga aagcagtacc gcagccggaa gggtaaacac
    11941 ccaggccatc agaatactgg ttacggtttt acgctgtaag ccgccgccat ctaccaccat
    12001 cgtccccgcg acagaagagg agagtacgtg agtagtggaa accggcatcc cggtataact
    12061 cgccaggccg atagacactg ccgccgtcat ctgggcagac atcccctgag cgtaggtcat
    12121 gcctttctta ccgattttct caccgatagt cgttgccaca cggcgccagc caatcatcgt
    12181 accgatacct aacgccagcg cgaccgccat gatgatccac accggtgcat actcgatggt
    12241 gctaagcatg tcggacttca gtttcttcaa caggcgctga tcgtcagcac tcacgccagg
    12301 catcttcacc actttgtcga tagtgtcaga aacgcacagc ataatgcggc gcatctggct
    12361 acgttgatca agcgacagct tgtcgtagct ttccacatcg gtggtcaaca tacctttcag
    12421 gcggttgagc gcgttaatgg tattcgacgg atggcagtgg aactccgcag gttgcgttgc
    12481 gccagcttcc ggagccggta ctaactgatc agcaccggta gcctgtttga gcagcgcagg
    12541 atgctgctca aagtaagctt cgacgttgtt gatggcatca cgggtacggg tgatttcgta
    12601 gccagtggca ttcatgttca ccacgaagcc tgctggcgcg acgccaatca ataccaacat
    12661 aaccagacca atgcctttct gaccatcgtt cgcgccgtgc gaaaacgcca cgccgatagc
    12721 ggaaaggatc agcgcgatac gcgtccagaa cggcggcttt ttcttgccgt ctttcttttc
    12781 acgctccgct ggggtcaggt ggatacgggc gcgtttcttg gtgccgctcc agtagcgacg
    12841 cagcaagaaa atcagaccgc cagcaaacac caggccgaca ataggggaaa cgatcagaga
    12901 accgaaaata cttaatactt tcgggatatt gagtgcatcc accactgacg tcccggtcat
    12961 caacgcattg gttaaaccaa tcccgatgat cgcgccaatc agcgtatgag agctggatgc
    13021 aggtaaacca aagtaccagg tacccaggtt ccagataatc gccgccagca acatagagaa
    13081 caccatggca aggccatgag acgatcccat attaagcagc agatccgtcg gcagcatatg
    13141 cacaatggca taggcaacac tcagaccacc cagcaaaaca cccaaaaagt tgaacaccgc
    13201 cgccataacc acggcgagct gagaacgcat cgcgcgggta tagataacgg ttgccacggc
    13261 gttggctgtg tcatggaaac cattgatggc ttcgtagaac agcacaaaag ccagtgcaag
    13321 caataataac agcccggtat gcaaatccag gccagcaaac aaatgtagca taggacgtta
    13381 cgccattttg aggacatgaa cgcggcgcat tatcagtgac tttcgcggcg cgggcaaagt
    13441 gaaatataga ctttttttga tttgcctcct gtatggattt cactcaaaaa ataattatct
    13501 tatataattc aggcaaatac ttccttttag taatattgat gctggtgcga ccactgagga
    13561 atctttacaa ttcacgcccg ttttttctaa gaggagcgca acgtggaaag gtttgatgcc
    13621 attattatag gcgctggtgc ggcgggtatg ttctgttctg cgctggcagg tcaggcagga
    13681 cgccgggttc tgctgatcga taatggtaaa aaaccagggc gcaaaatcct tatgtctggc
    13741 ggtgggcgct gcaactttac caacctttat gtcgaaccag gcgcttatct gagccagaat
    13801 ccgcattttt gtaagtctgc actcgcgcgt tttacccagt gggatttcat tgatctggtc
    13861 aataaacacg gcatcgcctg gcacgagaaa acgttaggac aactcttctg cgatgactcc
    13921 gcgcagcaga ttgtcgacat gctggtggat gagtgcgaga agggcaatgt aaccttcaga
    13981 ttgcgtagcg aagtgctgag tgtggcgaag gatgaaacag gcttcacgct tgaactgaac
    14041 ggcatgactg tcggttgcga aaagctggtc atcgcgaccg gtgggctgtc aatgccgggg
    14101 ctgggcgcgt cgccgtttgg ttataagatt gccgaacaat ttggcctcaa cgtgctgccg
    14161 acccgcgcgg gtctggtgcc attcactctg cataaaccgt tgctcgaaga gttacaggtg
    14221 ctggcgggcg tggcggtgcc ttccgtgatt accgctgaaa acggcatcgt tttccgtgag
    14281 aacttactct tcacccatcg cggcttgtct ggaccggcgg tgttgcagat ttccagctac
    14341 tggcaaccgg gtgaatttgt cagtatcaat ctgctaccgg atgtggatct cgaaaccttc
    14401 ctgaatgagc agcgtaacgc acatccgaat cagagcctga aaaacacact ggcggttcat
    14461 ctaccgaagc ggttggttga acgcttacag caactcgggc aaatcccgga tgtttcgcta
    14521 aaacagctca acgtgcgtga ccaacaggca ctgattagca cattgaccga ctggcgcgta
    14581 caacccaacg gcactgaagg ctatcgcact gccgaagtga cgctcggcgg cgtggacacc
    14641 aacgaactct cttcacggac gatggaagcg cgcaaagtgc ctgggctgta cttcatcggc
    14701 gaagtgatgg acgttaccgg ctggctgggg ggctataact tccagtgggc gtggtcgagt
    14761 gcgtgggctt gtgcgcagga tttgattgca gcaaagtcgt cctgacctat ttcctaaaga
    14821 tgaaaattat tagagtcgca ttaaaaatga gctaattttg atagtggtta tcttgtgatt
    14881 attttctaat gagcccgtga actgaaaccc tccatgctta atataaggtg gatggaaagg
    14941 tgattgaaaa ctcacccagt ggccagatct ttatgaaata tgaacaggtt gaaaaaaaca
    15001 gtaactttgc tgttttttat acttaaccac tatgcattaa tgctgcgtta tttcatgatg
    15061 cctaagaaaa accagcgtta cgcaaatggt caacgctggt tttatccggt acgttgcaat
    15121 tattttttag cagaacccgc ttctaatatt gaaacgattg agaacaacgt aaagcatacc
    15181 gctcccaaac cgaccagaac tcgcgaagga ataaaatatc ccatgtcggt ctgcgccatt
    15241 tccgcaagaa acgatgccag gaaaaggcaa aacaggcagg tgaagacggg aatcatcggt
    15301 attctgttgg ctaacgaaca ggtacgtctc cataccagtg ccagtagcca gacttttgag
    15361 aatatgctgt aacagatcat tccgaggcaa ataagaataa tgccgggagc cagtcgggcg
    15421 cttgcatcgg aactgactaa gacgtatatc ccctgcagta ccgtgattga gccgagaaaa
    15481 ataacccaat aacaccacag ccagtgttct ttagttgaaa acgtattacg tgtttgatgg
    15541 acaattgtgg caacaaggcc aattagacag gcacagattg cggttaaccc aagcaatacg
    15601 tgacccgcga cataatgcgg agttatgtca gcactacgta acagcgtaat agaccaaatg
    15661 aaaccgagca gggtgagtaa aacgggaacg gcaatgaggc aattaccgat taatgaagaa
    15721 taagcctgta ccggcgttcc atcgctcttg ctccccgctg catttttggg aatgagcaga
    15781 aagtgaccgg atgacgctgc caccgtcgat acacaggcgg caatcatacc aacgccgaaa
    15841 ataacatggc cggcgacaaa ctcgtctgcc atcacatcat tgcctgctaa cagtgcccaa
    15901 ccccatatca tggtgataat tgaccccgca tagccaataa tggggaacaa tgtattgtaa
    15961 aacgtattaa cgccgcgcgt gagctgcgaa atgataataa atgcggtagt gaataatgcc
    16021 aggcatatgg ccgccagaga aatcagcaca tgacccgcga cgaaatactc gctttgccct
    16081 gaactttcca gcacaaagcc acctaacgcg atgcaaatta atcccataat caaagggatt
    16141 aatttgaaaa gccacccgat atatatgttc actttaaact cctcaggtat ccctgctaat
    16201 aatgaaatgt gcattaatga taatttcagt ctgttaatat aacgacgaga tatcattaaa
    16261 gacaaatgca tagcataact atagcggggt ttactgtgcc gagtgccat
//
LOCUS       DBANUU010000095        15699 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000095 DBANUU010000000
VERSION     DBANUU010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.095"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..530
                     /locus_tag="ACIE68_003201"
     CDS             <1..530
                     /locus_tag="ACIE68_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFX5909474.1"
                     /translation="PEIILLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGEVRVPHR
                     GNVVDRVIEGAYEVVGVFDRIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVT
                     TADILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLN
                     RALWVMAETLLESLR"
     gene            831..1304
                     /locus_tag="ACIE68_003202"
     CDS             831..1304
                     /locus_tag="ACIE68_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFX5909475.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDGTWVLFNAMNGN
                     RAEMSPEAAGIAACLMAYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            1320..1796
                     /locus_tag="ACIE68_003203"
     CDS             1320..1796
                     /locus_tag="ACIE68_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFX5909476.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVSPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            1865..2086
                     /locus_tag="ACIE68_003204"
     CDS             1865..2086
                     /locus_tag="ACIE68_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFX5909477.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWYGGTEAYTSREVQ
                     DIPGVLAVFAERRKDSFGSYVRLMSVTLN"
     gene            2249..2623
                     /locus_tag="ACIE68_003205"
     CDS             2249..2623
                     /locus_tag="ACIE68_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFX5909478.1"
                     /translation="MSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRADIRGRFSDADSYHLEQAFPLLMKQLELMLTSGELSPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            2670..3047
                     /locus_tag="ACIE68_003206"
     CDS             2670..3047
                     /locus_tag="ACIE68_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFX5909479.1"
                     /translation="MNTLPDTHVRETSGCPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNDITLGKHPEAKQ"
     gene            3044..3532
                     /locus_tag="ACIE68_003207"
     CDS             3044..3532
                     /locus_tag="ACIE68_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFX5909480.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDIDGIELAELINVARDNDYS
                     LRVVDESDRTSADCTPPFAALTGIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            3544..3741
                     /locus_tag="ACIE68_003208"
     CDS             3544..3741
                     /locus_tag="ACIE68_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFX5909481.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIHTLRQVQLLQQNR"
     gene            3826..4674
                     /locus_tag="ACIE68_003209"
     CDS             3826..4674
                     /locus_tag="ACIE68_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFX5909482.1"
                     /translation="MSAITVSRPEVVTGHTDVICSTSVSHILAVRKTTLLQIDTLIRQ
                     LAEISVLTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYHSLEYDNFPEISEANIWSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGLHLITGQQADRLADLERMLHLFSGKPIPDSR
                     ENIIIRLDDHIRSVQGKVCYEDEMFNIRYFKKGSAHITFRKPELVDRLNDIIAKHYAG
                     KVSSSV"
     gene            complement(4733..4936)
                     /locus_tag="ACIE68_003210"
     CDS             complement(4733..4936)
                     /locus_tag="ACIE68_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909483.1"
                     /translation="MLSKQIPLGIYEKALPAGDELNQAILLNNKYIFKHLPINSPQYS
                     PQGVVVIHGGILNSVEIFSVMVQ"
     gene            complement(4946..5596)
                     /gene="ulaD"
                     /locus_tag="ACIE68_003211"
     CDS             complement(4946..5596)
                     /gene="ulaD"
                     /locus_tag="ACIE68_003211"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFX5909484.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5610..6074)
                     /gene="ulaC"
                     /locus_tag="ACIE68_003212"
     CDS             complement(5610..6074)
                     /gene="ulaC"
                     /locus_tag="ACIE68_003212"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFX5909485.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(6084..6389)
                     /gene="ulaB"
                     /locus_tag="ACIE68_003213"
     CDS             complement(6084..6389)
                     /gene="ulaB"
                     /locus_tag="ACIE68_003213"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFX5909486.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(6405..7802)
                     /gene="ulaA"
                     /locus_tag="ACIE68_003214"
     CDS             complement(6405..7802)
                     /gene="ulaA"
                     /locus_tag="ACIE68_003214"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFX5909487.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNIMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            8157..9221
                     /gene="ulaG"
                     /locus_tag="ACIE68_003215"
     CDS             8157..9221
                     /gene="ulaG"
                     /locus_tag="ACIE68_003215"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFX5909488.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9329..10084
                     /gene="ulaR"
                     /locus_tag="ACIE68_003216"
     CDS             9329..10084
                     /gene="ulaR"
                     /locus_tag="ACIE68_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFX5909489.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(10081..10830)
                     /gene="yjfP"
                     /locus_tag="ACIE68_003217"
     CDS             complement(10081..10830)
                     /gene="yjfP"
                     /locus_tag="ACIE68_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFX5909490.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGAGYFTSLARSLFPPLIPETTA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            11012..11341
                     /gene="bsmA"
                     /locus_tag="ACIE68_003218"
     CDS             11012..11341
                     /gene="bsmA"
                     /locus_tag="ACIE68_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFX5909491.1"
                     /translation="MFSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11490..11765
                     /gene="yjfN"
                     /locus_tag="ACIE68_003219"
     CDS             11490..11765
                     /gene="yjfN"
                     /locus_tag="ACIE68_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFX5909492.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(11882..13507)
                     /locus_tag="ACIE68_003220"
     CDS             complement(11882..13507)
                     /locus_tag="ACIE68_003220"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFX5909493.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELRREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13591..14754)
                     /locus_tag="ACIE68_003221"
     CDS             complement(13591..14754)
                     /locus_tag="ACIE68_003221"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFX5909494.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLAPWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(14757..15395)
                     /locus_tag="ACIE68_003222"
     CDS             complement(14757..15395)
                     /locus_tag="ACIE68_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFX5909495.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESSDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVISGFLLSRVIRKDRDEQFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(15405..>15699)
                     /locus_tag="ACIE68_003223"
     CDS             complement(15405..>15699)
                     /locus_tag="ACIE68_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFX5909496.1"
                     /translation="EWQLIKNNNTAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWG
                     GIALVIQLLVFAGVRLYMPALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
BASE COUNT         3962 a         4242 c         3895 g         3600 t
ORIGIN      
        1 tccctgaaat tattctgctc aactctcatg acggttcatc cagctaccag atgttacccg
       61 gatatttccg tgccatttgt accaatggcc tggtctgcgg tcagtctctg ggagaagtgc
      121 gtgttccgca ccggggaaat gtggtggaca gagtcattga aggggcttac gaggtggtgg
      181 gcgtgtttga ccggattgag gaaaagcgtg atgccatgca gtcgctgatt ctgccgccac
      241 cggcacgcca ggcgctggca caggcggcac tgacttaccg ttatggtgac gaacatcagc
      301 ccgtcaccac cgctgacatt ctgacaccgc gacgcaggga ggattacggt aaggacctgt
      361 ggagtgctta tcagaccatc caggagaata tgctgaaagg cgggatttcc ggtcgcagtg
      421 ccagaggaaa acgtatccac acccgtgcca ttcacagcat cgacaccgat attaagctca
      481 accgcgcatt gtgggtgatg gcggaaacgc tgctggagag cctgcgctga tgcagcctgt
      541 ccctgaaggc gctgccggga tgtcgttcca tttctgatga tgacctgtgc gcctggtgta
      601 cccgtctgct ttaccgtccc ggagaaatca gtctgtgcag actgagtaca ggcaatggca
      661 tctggccatc cgtctgtgac cggaacggtt acgcgtacgg ctgtccggga tttcagccga
      721 atatcattca tccctgatat taccgggtct cacggtcagt gatgaacgtg atgttgccag
      781 ctcccgacct ttactccctc tcatttattc acattacaag gatttcatac atgaaaacat
      841 tgtctcagaa caccacctct tcggcctgtg caccggagac tggcctgcaa caactggttg
      901 ccaccatagt ccctgatgaa cagcgcatca gtttctggcc gcagcatttt ggcctcattc
      961 cacagtgggt gaccctggag ccccgtgtct tcggctggat ggaccgtctg tgtgaagact
     1021 actgcggtgg tatctggaat ctgtacaccc tgaacaacgg tggcgcattt atggcacccg
     1081 aaccggatga cgatgatgac ggaacatggg tactgttcaa tgccatgaac ggtaaccgcg
     1141 ctgaaatgag tccggaagct gccggcattg ccgcctgtct gatggcgtac agtcatcacg
     1201 cctgtcgtac agagtgttat gccatgacgg tccactatta ccgtctgcgg gattacgccc
     1261 tgcagcatcc ggaatgcagc gccattatgc gcatcatcga ctgaatggag gcaggaacaa
     1321 tgcagcagct ttcctttcta cccggagaga tgacgcccgg cgagcgcagc ctcattcagc
     1381 gggccctgaa aaccctggac cgtcatcttc atgaacctgg tgtggccttc acctccaccc
     1441 gtgcggcacg ggaatggctg attcttaaca tggcgggact ggagcgtgaa gaattccggg
     1501 tgctgtatct gaataaccag aatcagctga ttgccggtga aaccctcttc accggcacca
     1561 tcaaccgcac ggaagtccat ccccgggaag tgattaaacg cgccctgtac cacaatgccg
     1621 ctgctgtggt gctggcacac aatcacccgt ccggtgaagt ctcacccagc aaggcagacc
     1681 gccttatcac cgaacgtctg gtacaggcac tgggcctggt ggatatccgg gtgccggacc
     1741 atctgatagt cggtggcagc caggttttct cctttgcgga acacggactg ctttaacccg
     1801 tcaccgtcac aaccacatca cacctgtttt cacttttatc ttctgtcttc agaggtatca
     1861 cactatgaaa atcatcaccc gtggtgaagc catgcgtatt caccaacaac atcctgcatc
     1921 ccgtcttttt ccgttctgca caggtaagta ccgctggtac ggtggcactg aagcgtatac
     1981 cagtcgtgaa gtgcaggata ttcccggtgt gctggccgtg tttgcagaac gccgtaagga
     2041 cagttttggc tcgtatgtcc ggctgatgag cgtcaccctg aactgatgtt ccgtcacccg
     2101 gaaaacgagt acgaccagat ggactcagac gccgctgtgg atgaggccct gctcatcaac
     2161 gaatacacct ttaccgaagc cggtctgcac gccggctgac ctgtcatcgt taaaccattc
     2221 ttaaccgatt ttctgtgagg attttatcgt gtcagacaca ctccccggga caacgcttcc
     2281 cgacgacaac aaagaccgcc cctggtgggg gctgccctgc accgtgacgc cctgtttcgg
     2341 ggcacgtctg gtgcaggagg gtaaccggtt gcattacctt gcagaccgcg ccgatatcag
     2401 aggccgcttc agcgacgcgg attcgtacca cctggagcag gcctttccgc tgctgatgaa
     2461 acaactggag ctcatgctca ccagtggtga actcagcccc cgctatcagc ataccgtcac
     2521 gctgtatgcg aaaggactga cctgcgaagc cgataccctg ggctcctgtg gttacgttta
     2581 tctggctgtt tatccgacgc cggcacccgc aaccacctca taaccccgaa actctcacct
     2641 ttagcactac cagttacaga gaacttaaaa tgaatacatt acccgacact cacgtacggg
     2701 agacatcggg ctgcccgtct ccggttgaaa tctggcagac actgctcacc cgactgctgg
     2761 accagcacta cggcctcacg ctgaatgaca ctccgttcgc tgatgaacgt gtgattgagc
     2821 agcatattga ggccggcatt tcactgtgcg atgcagtgaa ctttcttgtt gaaaaatacg
     2881 cactggtgcg taccgaccag ccggaattca gtgcctgtac ccgttctcag ttaataaaca
     2941 gtattgatat cctccgggca cgccgggcaa ccggcctgat gacccgcgac aactacagaa
     3001 cggtaaatga cattaccctg ggtaagcatc cggaggcgaa acaatgaaac tggccctgac
     3061 gctggaagcc gacagcatta acgtgcaggc actgaacatg gggcgcattg tcgttgacat
     3121 cgatggtatt gagcttgccg aactgattaa cgtggcccgc gacaatgact actcccttcg
     3181 tgttgttgat gaatctgacc ggacctcagc agactgcacg ccaccatttg ctgcccttac
     3241 cggcatacgc tgcagtaccg cacatatcac ggaaacggac aacgcctggc tgtactcgct
     3301 gtcacaccag actaatgacg acggcgaaac agaatggatt cattttacag gcagcggata
     3361 tctgttacgt accgatgcgt ggtcataccc ggttctgcgg cttaagcgcc tgggcctgtc
     3421 aaaaacgttc cgtcgtctgg ttgtcacact tacccgacgt tatggcgtca gtctcattca
     3481 tctggatgcc agcgctgaat gcctgccggg tttacccact ttcaactggt aaccaggaac
     3541 aacatgaaat cattaaccac ggaaacagca ctggatattc tgattgcgtg gctgcaggac
     3601 aatatcgact gcgaatcggg aattatcttt gacaacgatg aggataaaac ggattcggca
     3661 gcactgctgc cctgcattga acaggcgagg gaggatatcc ataccctgcg ccaggtgcag
     3721 cttctgcaac agaaccggtg agtcttactc accagacttc attccactga cgccagcctg
     3781 aacagggctg gcgttttcat ttatctgcga aaaggaatat cgattatgtc tgcaatcaca
     3841 gtctcccgtc cggaagtggt caccgggcat acggacgtta tctgctccac ctcagtcagt
     3901 cacattctgg ctgtgcgaaa gactacactg ctgcaaatcg acacacttat ccggcaactg
     3961 gctgaaatct cggtactgac agaaagtatt ggaggtaaaa ctgctctgga ctgggccatg
     4021 aaacaggatt ttcgctgtgg ctgctggctc atggagaaac cggaaaccgc aatgaaagcc
     4081 atcacccgca accttgaccg cgaaatctgg cgtgacctga tgcaacgttc cgggatgctg
     4141 tcattaatgg atgcgcaggc ccgtgataca tggtaccact cactggagta cgataacttt
     4201 ccggaaatca gtgaagcgaa catttggagc acatttgagc aactgcacca gaataaggat
     4261 gaagtgtttg agcgaggagt gatcaacgtc ttcagagggc tgagctggaa ttacaaaacc
     4321 aattccccct gtaaatttgg cagtaaaatt atcgtcaaca acctggtgag gtgggacaga
     4381 tggggattgc atcttatcac cgggcaacag gcagatcgac tcgccgacct ggaaagaatg
     4441 ctacacctgt tcagcggcaa accgatcccc gacagccggg aaaatatcat cattcggctg
     4501 gatgatcaca tccgctccgt tcagggcaaa gtgtgctatg aagatgagat gttcaacatc
     4561 agatacttta agaagggctc cgcacacatc acgttcagga aaccagaact ggttgatcgg
     4621 ctaaatgata ttattgcgaa acattatgcg ggaaaagtat cttcttcagt atagacttat
     4681 atatcacagg tattgtgtct cgtccggaac aagacaggta ggtatgctgc attcactgaa
     4741 ccattacaga gaatatttca acagagttca atatacctcc atgtataaca actacacctt
     4801 gggggctata ttgggggcta tttataggga ggtgtttaaa tatatactta ttattcaata
     4861 atatagcttg attaagttca tccccggcgg ggagcgcttt ttcatagatg ccaagcggga
     4921 tttgtttgga caacatatcc gctccttagc cccacagttc ggcgatggaa cgtttgaact
     4981 gacgtgcagc ttcaaccgga gaagcggcat cacggatgct gcgaccagcg ataaacacat
     5041 gaatcggaat acctttgaac agcggcagat cttccagcgc caggccaccg gtgacggtga
     5101 ctttgaagcc catatcggaa agacgtttga tcgcggtaat gtccgcttcg ccccacgcta
     5161 cgcctgcggc ctgcgcgtca cggctgcggt gataaaccac ctgctgaatg cctgcgtcac
     5221 gccactgctg cgcctgttcc caggtccagt agccggtcaa ttcgatctgc acgtcgccgt
     5281 taaactcttt tgctacgtcc agcgcgcctt tggcggtgtt gatatccgca cagcaaatta
     5341 ccgtcaccca gtcagcgttg gcttcgaagc acatacgcga aaggattttg cccgcatcag
     5401 caattttagc gtctgccagt acgattttgt gcgggtagag cgctttcagg tcacgaaccg
     5461 cacgcacgcc ttcgcccacg cacagaatgg tacccacttc gataatgtcg acttcttcgg
     5521 caatcaggcg agtggtttcg taggcgctat ccatagtctg gttgtccagc gcgacttgca
     5581 acatcggtaa tgacatcttc aattccttct taagctgccg cgttggtgcg gtcaatgaga
     5641 tccagtactt cctgctcggt acggcaggca cgtaaacggt cgaaattttc ttcatcttca
     5701 aacaggttga cgatctgcat gatgcccact tcctggtgag tgttggcatc gaccgccgcc
     5761 atggtgatga gtatatccac cgggtcatta tcttcgtggt tgaactccag cggctttttc
     5821 agcgtcacca gcgcaaaacc ggttttctta acaccctctt ccgggcgacc gtgcggcatt
     5881 gccaggcctg gtgcgagcac aaagtacgga ccgtgctgtt caaccgcatc cagaatcgcc
     5941 tggtagtaac gcggctcgac aacatccgcc gccaccagca ggtcaacgcc gatttttact
     6001 gcgtcctgcc atgtctcagc ttcagcctgc aggcggatgg atttattctc cgccagcgaa
     6061 tcacgtaatt tcatggtgcg tccttacttc acatcctgcg ggaaatgctc tttgatcact
     6121 tccagcagtt tcgggccaaa gtcagcagga gagagcatgt tgcgcacgcc aaccacgtat
     6181 ttgttgccgg tcacggtgat ttcgcccgca atgtgcgtag aagcgatgat gatatccgcg
     6241 ccgctcaact cgcttttgta ttcgccaacc gcgcagctgt ttaccgtgtg gtcaatgttt
     6301 gattgggtta aaaactggtc cactttcatc ttcatgatca tggaactgcc ttgtccgttg
     6361 ccacacacag ccagaatacg tacggtcata atcaaaactc cttattaagc agactgttct
     6421 gccagttgtt tttctgcatc ttcttctgcg cgcagtgcgc ggccagcgaa gaacatataa
     6481 gccagtgcaa ttacaatgat gacggccata aaggcgatac cgatggagaa gaagccctgc
     6541 atcatcggcg gtgccagaat cgaccagtcc gccataccca tccaggcact cataccggta
     6601 agtttcaccg cccagacgca accaaagatt tcgatcatcc ccatcaccag acaaatcttc
     6661 agcgccgcac gccagccgcc gaagtggtta gcgaacacgc cgatggtggc gttggagaag
     6721 aacatcggga taaagccagg aataatcagg attgaggaac cgcaggcgac caggatgcca
     6781 accgcaatca gctgaccgat ggtgccccac ataaaccccc agaccacggc gttcggcgcg
     6841 aagctataga tagccgcaca gtcaatcgcc agaaccgcac ctgggatcag gcgctgggaa
     6901 atgccgttaa atgcttcaga gagttccgcc acaaacatgc gcacaccctg agtgatgatg
     6961 aagatcgcca ccgcgaagga gaaaccagtt tgcaggatgt acaccgtcca gttcactttg
     7021 cctgccatcg cctgcacggt gtcgataccg aaggagagca gaatggcacc aaagaagatg
     7081 gtcatcacaa tcgccgtgga gacgatgttg tcgtggaaaa tattcagcca gcctggcagt
     7141 ttgaggtctt caacgctttc ctcttttttg ccgaggaacg gcgcaacttt ataggcaatc
     7201 catgatgcaa actgttgctg gtgaccgatg gagaaaccgc agccgtccgt cacttcctga
     7261 gtcggcttgt acattatgtt ggaggtaatg ccccagtaga gcgaaaccag aatcgccgtg
     7321 cagataatgg tcgtccacat ggagtagccg aaaataaaca acgtaacggc aatcagcccc
     7381 gcctgctgga acatgatatg accagtcagc atgatggtac gaataccggt aatgcgacgc
     7441 agcagcacgt aacagatgtt caacgccagc gctaacaaca ccgcgtaacc tacccagcta
     7501 taggcatcgc ccatgcggtc gatggttgcc atcatcgacg cgtaagtatc ggaaattgcg
     7561 ccgttaatgc cgtagacttc ggacattttc gccaccaccg gtttgaaggt gctggtgagg
     7621 atgccggacc ctgcctgcaa caacatgaaa ccaattatgg ttttaatcgt gcctttaata
     7681 ataacgctga cacttttgcg cagtaggatg tagcccagac aggtcacaat acccagcaac
     7741 aacggggcat tggtcatgac ctggttaaaa aacacggtaa agatgttgta gaggatctcc
     7801 ataacgatct cctgaagaag aggtaaccgg gtattccaca cacccggaat gttgtgcact
     7861 cactctaatt ttcaaaagta atcacaacaa gattatttat gattaaatgt gacgcgaccc
     7921 gcaaataatt ccacatggat agaatgggat tcacacaacg aaacaactat tcatttaaat
     7981 tcatttagtt aattgaatta cacattccat taaatctttc cacactcaaa atcaacaacc
     8041 acttccctgg caaaaagtca aaaaatggtc attgccttcc tgaaaaaata gtgtcaggat
     8101 taatcaaaac caatcacata ttgattcgaa ttgaactatc atgaaggtaa atggcgatga
     8161 gtaaagtgaa aagtatcacc cgagaatcct ggatcctgag cactttcccg gagtggggta
     8221 gctggctgaa tgaagaaatt gaacaagagc aggtcgcccc tggcacattt gcgatgtggt
     8281 ggctaggctg caccgggatc tggttgaaat cagaaggtgg caccaacgtt tgcgttgatt
     8341 tctggtgcgg cactggcaaa caaagtcacg gtaacccgtt aatgaaacag ggtcaccaga
     8401 tgcagcgcat ggctggcgtg aaaaaactgc agccaaacct gcgcaccacc ccgtttgttc
     8461 ttgatccgtt tgcgattcgc cagatcgacg cggtactggc gactcacgat cacaacgatc
     8521 atatcgacgt taacgttgct gctgccgtga tgcagaactg tgctgatgac gtaccgttca
     8581 ttggaccgaa aacctgtgtg gatttgtgga ttggctgggg cgtaccgaaa gagcgttgca
     8641 tcgtggtcaa accgggcgat gtagtaaaag tgaaagacat tgaaattcat gcgcttgacg
     8701 ctttcgaccg tactgcactg atcaccctgc ctgccgatca aaaagcggct ggcgtactgc
     8761 cagatggcat ggacgatcgc gcagtaaact acctgttcaa aacccctggc ggttccctat
     8821 atcacagtgg cgattcccac tactctaact attatgcgaa gcacggtaac gaacatcaga
     8881 tcgatgtggc gttaggatcg tatggtgaga acccgcgcgg tattaccgac aaaatgacca
     8941 gcgccgatat gctgcgtatg ggtgaagccc tgaatgcgaa agtggttatc ccgttccacc
     9001 acgacatctg gtcaaacttc caggccgatc cgcaagagat ccgcgtgctg tgggagatga
     9061 aaaaagatcg tctgaagtat ggcttcaagc cgtttatctg gcaggttggc ggcaaattta
     9121 cctggccgct ggataaagac aacttcgagt accactatcc gcgcggtttc gatgattgct
     9181 tcactattga accggatctg ccgttcaagt cattcctgta atctgtcgtt tataccggat
     9241 agcgtggaga agttcgcgct atccggcgta gtatttcctg gctatttcaa atatcatcta
     9301 aaaaaatcaa attttatcgg aatagctcat gactgaagca caaagacatc aaatcctcct
     9361 ggaaatgctc gcacaattgg gctttgtgac cgttgagaaa gtcgttgagc gtctgggaat
     9421 ttcgcctgcc actgcgcgac gcgatatcaa taaacttgac gaaagcggca aactgaaaaa
     9481 agtgcgcaat ggcgcagaag ctattaccca acagcgcccg cgctggacgc cgatgaatct
     9541 gcatcaggcg cagaatcacg atgaaaaagt acgtatcgct aaagcggcct cgcagctggt
     9601 taatccgggc gaaagcgtag tcatcaactg cggctccacc gcgtttctgc ttgggcggga
     9661 aatgtgtggc aagccagtgc aaatcatcac taattatcta ccgctggcaa attacctgat
     9721 cgatcaagaa catgacagcg tgatcattat gggcggacag tacaacaaaa gtcagtccat
     9781 cactttaagc ccgcagggca gcgaaaacag tctctatgcc gggcactgga tgtttaccag
     9841 cggcaaagga ctgacagcag aagggttgta taaaaccgat atgctgacgg caatggcaga
     9901 gcagaagatg ctgagcgtgg tagggaaact ggtggtactg gttgatagca gtaagattgg
     9961 cgaacgcgcg ggaatgcttt ttagccgtgc cgatcaaatt gatatgctga tcaccggcaa
    10021 aaatgctaac ccggaaatcc tgcaacaact ggaagcgcaa ggggtcagca ttctgcgtgt
    10081 ttaaagatgc tggcggaaaa atgtcactgc agcatctaac gcctcaggcg taatgcggtg
    10141 acgcacgcct ggctgccatg aacaggttag cagtttatcc cgtcccgtct cgcttaaggc
    10201 ctgctgcaaa cgtagtgatt cgtcggcagg cacaacgtca tcgtcgaggc catgccacag
    10261 cagtagaggt ctgtcactaa gttgttccag gtggtttgtc gcttcccact ctgccagcgg
    10321 cgcgacaatg ttattgaatt cattctgctg tgctgtcgtt tcaggtatca gcggtggaaa
    10381 cagtgaacgg gcgagtgatg taaaatagcc cgctcccatc atgctggcgg tacatctcac
    10441 cgtggggtgg cgagcggtaa tccccagtgc cgtcatcgcg cccatcgacg caccaccgac
    10501 tgccagacgg tcatcaagca gccagttttc ttcggctatt gccgcacgta aagtagtgaa
    10561 ttcctgcata ctttgtagca agatttgcca gaattgattt aaccgccgcg ctgcgtcacc
    10621 actaaaacgg ctaccgtgat cgggcgcatc cggcatgatc acccgcaaac cagcctgcgc
    10681 cagcgcaacg gcaaaatagc tatacaccag actggatgaa gtaaagccgt gataaaaaat
    10741 tacgcacggt aacggggtat ctttttgccc gacaggataa gcatgaagaa cgggaatatc
    10801 tgccagctcg cgtgattcta tttcaatcat ctgtctctcc tttttgtctg gtatgacatg
    10861 aagaaatcct tagcacaagt ttctgctggc ggtattagtt cataatgttg agatgtgggt
    10921 tacgctttcg tcacgttttc attcgaaaat cgcgtaccag gtaacaattc gggaacattc
    10981 ccccaaagtt aaattaataa ggcactacac tatgtttagc aggaaacgta atagcgttat
    11041 ttaccggttt gccagtttat tattggtgtt gatgttaagt gcctgtagcg cactgcaagg
    11101 tacgccacag ccagcaccgc cagtaacgga ccatccgcaa gagattcgcc gcgaccagac
    11161 gcaagggtta cagcgaatag gcagcgtaag cacgatggtt cggggttctc cggatgacgc
    11221 attagcagaa atcagagcga aagctgtcgc tgcaaaagct gattattacg ttgtcgtcat
    11281 ggtggacgaa accatcgtga caggacagtg gtattcacaa gccattttgt accgtaaata
    11341 aatcagacaa cttacatagt cagctttaca ttgctttgcg tccctatgcg tttacctgtg
    11401 cacaataaat taccggctgg aagcctgggc gtttattacc gcgatggaat gccctgcaac
    11461 atgtggggaa acgaaaaatg gagctgacga tgaaacaatt acttgcctca ccctcgctgc
    11521 aattagtgac ttatcctgcg agcgccacgg cacagtctgc agaattcgcc agtgctgact
    11581 gcgtgacggg cctgaatgaa ataggtcaga tctcagttag caatatttca ggggatccgc
    11641 aggacgtgga acgtatcgtg gcgttaaagg ccgatgaaca aggtgcgtca tggtaccgca
    11701 ttattaccat gtacgaagac cagcagcctg ataactggcg cgtacaggcg atactgtatg
    11761 catgagtgtc atctacagca aatgaacaat gccggatgcg gcgcgagcgc cttgtccggc
    11821 ctacaatttt cacatatttc atttagttaa tcgaaaccag cgtcgcatca gtcgtatacg
    11881 cttacacaca cactcccccc gtcgcccgca gcaataaatc attctggatc tgctctgaca
    11941 accgtacgcc gccgcgcgta tctaacatca cctgacacca cgcctgcgcc attggcggag
    12001 aagcatattt caacatttgc gcaccgcagc ccagcaggaa tagctgatga gtaatctctc
    12061 gcctcagttc ttcagctggc ttacgcagct gctgctgtaa acgacgaacc gcgcgatcaa
    12121 aatagcgatc ctgccctttc acttccacaa acgcttccga caataagtcg tatacgcccg
    12181 cttgcttatt gagaacgcgc agcacatcca ggcacataat attgccggaa ccttcccaaa
    12241 tactgtttac cggcatctcc cggtaaagcc gcggcagctc gctctcctcg caataaccaa
    12301 tgccgcccag cacctccatc gcttcggcca caaacggcat acctcttttg cagatcacaa
    12361 attttgccgc aggcgtaaat aaacgcgccc acagggcttc tttggcatcg gcacgccggt
    12421 cccacgctcg cgcaagacga aacagcaacg ccgtttgccc ttcaagctga agtgccatgc
    12481 gacttaagac atgacgcata aggggctgtt ggatcaatgg attaccaaaa acatggcgtt
    12541 gatgtgcatg ataaatcgcc agcgaaaatg cacggcgcat catggcatgg ctacccaggg
    12601 cgcaatcaaa acgcgtcatc ccacccattt tcaggatcag acgaattcct tccccttcct
    12661 gccccaacaa ccagccaatg gcatcctgaa actccacttc gcagctggcg ttagagcgat
    12721 tacccagctt atctttcagc cgctcgaggc gaatcgcgtt gcgttgcccg tcaggcaaaa
    12781 agcgcggcac aaaaaagcag gacaaaccgc ccgtagtctg cgccagcacc agatgcgcat
    12841 cgctttgcgg caccgagaaa aaccatttat gccccaccag ccgataagag ccatcttcca
    12901 ggcgctctgc gcgggtggtg ttgctcatga catcggaacc gccctgcttt tccgtcattc
    12961 ccatgccaat caacaaaccg cgtttttgcc cacctggcaa taagtgagaa tcgtagcgat
    13021 cgctcagcag cggcgtggtc cagtcctgaa acggcgcggg taacatctgt aacagcaatg
    13081 gcgtggcggc aaaggtcatg gttatcggac ataacgaccc tgcctcaacc tgtgcgtgca
    13141 acataaaacg cgccgcgcgc gccacaaatg cgccggagcg agcgtcttct tcccaggcaa
    13201 gattgtgcac ccgattggta catagcgcct gcatcagcag gtgccaggcg gggtgaaaac
    13261 gcacatcgtc cagacggcgt ccttgcgcat cgtagcgcaa taattcaggc ggattcacat
    13321 tcgccagccg ccccagttca agggattcag ccgttcctaa ctgctgacca atactggcta
    13381 gaaaatcgct atcccagcca gcaccttcac gcgttaccgc ttcgcagagc gcgccatcag
    13441 acaggtataa gttgctgtta tttaatggta taggttgatt aaaaacggtg tgagtttgcc
    13501 agtgcactgt gtctccctcc atcaatggca gtcaccatta gtatggtcac tgccatggat
    13561 tcatgacaga aagagattgc taaaacattc ttatccagca atgtaatgcg gaacgaaacg
    13621 tgaggtgtct ttggtgatca gtgtgttatc ttcacggatc cccattccgc acgcttcatc
    13681 atcgacaatc cagctaccga tgagtgtgta gctatcgcca aatcgcggca gaggttgaaa
    13741 cgcctggtag atcatcggtt catcggcgta atcaccatca gcgtggtcaa cgacgttatt
    13801 ctgaccgtcg aaaatggtga cgtttccgcc ttcgcgcgag tagatcggtt tacgcacata
    13861 gctttcgcca gcggcaatct gcgatttttc accctcaaac cagggtgcaa gaaggttagg
    13921 atgaccaggg aagaagcgcc aaagcagcgg cattagccct ttattactca agatactttt
    13981 ccataacggc tccacccatt gctcgcgacg cttgcacagc agcggaccgt tatcgtcacg
    14041 catcatccac tccagcggat acagcttaaa tgcacgctgg atgacattat catcaagatc
    14101 ggtcagtaca ccgccgacgc ccaaaccgag atcttcaatg tagataaacc gcgactcctg
    14161 ccctgcctgc tgggcgcagt cctgcaagta cagcacggta gtcctgtctt catcggtgtc
    14221 ctgacagcag cagaaataaa acggctcccg gctataaagc tcgctaaagc gtgaaatcag
    14281 gcgttcctga atggcattgt actgatcggc atcacgcgga ataatgccgc tgcgccgggc
    14341 atcctccagc cacaaccact ggaaataagc cgactcatac aacgaggtcg gcgtatcggc
    14401 gttgtactcc agcagcttca ccggcgcatt gccacaccag gcaaaatcca tccggccata
    14461 cagcgaagga tcgcgtgcgc gccagctttc ggcgatcaca tcccagtaca acggcgggat
    14521 cgccagttgc gtcaggatct cttcgtcttt aaccgcgcga tccaccacct caaggcacat
    14581 ctgatgcagt tccgcagtcg gtttttcgat ctgctcttca atctggcgca gagtaaaacg
    14641 gtaagcccga ctctcgtccc aatagatttc attgtcgatg atatgaaagt cgaaaccgtt
    14701 gttagcggcg atctggtcca gatcccgtcg cacaggaacg ttgtgtctca gcatgattag
    14761 cctccccaat gcccacgggc gctggaagaa cgaccatagc cgccgcgcga aacggttgac
    14821 gcttttttgg tggtaaagcc gcctgaagag taagactctt ttttgccaga accggagcgc
    14881 caggagtaat caccggaagt gttgcgccag acggggcggg aagcaaatga ggatccgccg
    14941 ctgttataaa caaactgctc atcgcgatct ttacgaataa cccggcttaa caaaaatcca
    15001 gatattaccg ggatccaact ctgttcaacg ttgtcgtagt aacaattttc atacttagac
    15061 tgacagttct gctgagtcat attcttcgga acatcggcat aaaacgccgc tttggcgtta
    15121 ttccagccac gcgcgcaaat gtcggcatta ttaccgtcat caatacaatc ctgcactgtc
    15181 gagtaaaacg ttccgtcgcc gtcgttatcg acatcgctac tttcactcca tcctttgact
    15241 attaaaaaaa cggcaccgcc cattagcacc aacgttaagt atttttgtgc gtagcgattg
    15301 cggcgcggct caaaaggatt atgcggtctt ccaatgcgac tgatcgctcc gtgaccaatt
    15361 ttactgttat ttcttgattt gcgttttctg gccatgatcg tccattacca tgtcatacaa
    15421 gctgcgttaa aaataccgcc agccagcgcg gcggttccca taaacattcc tgctgcggta
    15481 ttgtgattaa taattttttc gcttaatgcg ggcatataaa gtctgacacc agcaaaaacg
    15541 agtaactgaa tcaccagtgc aatcccgccc caggcgaaat agtctggaat actcaccgca
    15601 ttgattgccg cactggataa tgggataacg taacctaaca atgtaccact gaacgccagt
    15661 gaagccgcgg tattattgtt tttgattaac tgccattcg
//
LOCUS       DBANUU010000096        15693 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000096 DBANUU010000000
VERSION     DBANUU010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.096"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            35..553
                     /gene="yfiR"
                     /locus_tag="ACIE68_003224"
     CDS             35..553
                     /gene="yfiR"
                     /locus_tag="ACIE68_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFX5909497.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            543..1769
                     /gene="dgcN"
                     /locus_tag="ACIE68_003225"
     CDS             543..1769
                     /gene="dgcN"
                     /locus_tag="ACIE68_003225"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFX5909498.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISITSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1785..2267
                     /gene="yfiB"
                     /locus_tag="ACIE68_003226"
     CDS             1785..2267
                     /gene="yfiB"
                     /locus_tag="ACIE68_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFX5909499.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2343..2690)
                     /gene="rplS"
                     /locus_tag="ACIE68_003227"
     CDS             complement(2343..2690)
                     /gene="rplS"
                     /locus_tag="ACIE68_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFX5909500.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2732..3499)
                     /gene="trmD"
                     /locus_tag="ACIE68_003228"
     CDS             complement(2732..3499)
                     /gene="trmD"
                     /locus_tag="ACIE68_003228"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFX5909501.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3530..4078)
                     /gene="rimM"
                     /locus_tag="ACIE68_003229"
     CDS             complement(3530..4078)
                     /gene="rimM"
                     /locus_tag="ACIE68_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFX5909502.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4097..4345)
                     /gene="rpsP"
                     /locus_tag="ACIE68_003230"
     CDS             complement(4097..4345)
                     /gene="rpsP"
                     /locus_tag="ACIE68_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFX5909503.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4482..5843)
                     /gene="ffh"
                     /locus_tag="ACIE68_003231"
     CDS             complement(4482..5843)
                     /gene="ffh"
                     /locus_tag="ACIE68_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFX5909504.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6010..6801
                     /gene="ypjD"
                     /locus_tag="ACIE68_003232"
     CDS             6010..6801
                     /gene="ypjD"
                     /locus_tag="ACIE68_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFX5909505.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTALFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6822..8108
                     /gene="yfjD"
                     /locus_tag="ACIE68_003233"
     CDS             6822..8108
                     /gene="yfjD"
                     /locus_tag="ACIE68_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFX5909506.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8163..8756)
                     /gene="grpE"
                     /locus_tag="ACIE68_003234"
     CDS             complement(8163..8756)
                     /gene="grpE"
                     /locus_tag="ACIE68_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFX5909507.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            8879..9757
                     /gene="nadK"
                     /locus_tag="ACIE68_003235"
     CDS             8879..9757
                     /gene="nadK"
                     /locus_tag="ACIE68_003235"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFX5909508.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            9843..11504
                     /gene="recN"
                     /locus_tag="ACIE68_003236"
     CDS             9843..11504
                     /gene="recN"
                     /locus_tag="ACIE68_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFX5909509.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11653..11994
                     /gene="bamE"
                     /locus_tag="ACIE68_003237"
     CDS             11653..11994
                     /gene="bamE"
                     /locus_tag="ACIE68_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFX5909510.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12056..12346)
                     /gene="yfjF"
                     /locus_tag="ACIE68_003238"
     CDS             complement(12056..12346)
                     /gene="yfjF"
                     /locus_tag="ACIE68_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFX5909511.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12336..12773)
                     /gene="ratA"
                     /locus_tag="ACIE68_003239"
     CDS             complement(12336..12773)
                     /gene="ratA"
                     /locus_tag="ACIE68_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFX5909512.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            12944..13426
                     /gene="smpB"
                     /locus_tag="ACIE68_003240"
     CDS             12944..13426
                     /gene="smpB"
                     /locus_tag="ACIE68_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFX5909513.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13641..14003
                     /gene="ssrA"
                     /locus_tag="ACIE68_003241"
     tmRNA           13641..14003
                     /gene="ssrA"
                     /locus_tag="ACIE68_003241"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14188..15381
                     /locus_tag="ACIE68_003242"
     CDS             14188..15381
                     /locus_tag="ACIE68_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFX5909514.1"
                     /translation="MARTTRPLTNTEVLRAKALEKDLTLHDGDGLFLIVKTSGKKLWR
                     FRYQRPTTKQRTMMGLGAFPALSLADARRLRADYLALLANGIDPQIQAEVAEEEQQIA
                     LDSIFSTVAANWFQLKSKSVTPDYAKDIWRSLEKDVFPAIGEIPVQQIKARTLVEALE
                     PIKARGALETVRRLVQRINEIMIYAVNTGLIDANPASGVGMAFEKPKKKNMPTLRPEE
                     LPKLMRSLVMSNLSVPTRCLIEWQLLTLVRPSEASGTRWAEIDLEAKLWTIPAERMKA
                     KREHIVPLSSQALNILEVMKPISAHREYIFPSRNDPKQPMNSQTANAALKRIGYAGKL
                     VAHGMRSIASTAMNETGFNSDVIESALAHIEKNEVRRAYNRALYLEQRKELMQWWGNF
                     IKKAM"
     gene            complement(15409..>15693)
                     /locus_tag="ACIE68_003243"
     CDS             complement(15409..>15693)
                     /locus_tag="ACIE68_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFX5909515.1"
                     /translation="LLGDICYRECVDLKDAIYVGDSIIKDISMANSAGIESILALYGK
                     QHNPDYWNVLVSITHWSADDVQRESKLKELYSHVIPTYSINKFSEILDVI"
BASE COUNT         4017 a         4108 c         3694 g         3874 t
ORIGIN      
        1 atgcatttcg agcgaagatg gtggggatcc ctgaatgcgt ttttctcacc gactgttctt
       61 acttctgatt ctcctgctga cgggagcacc aatactcgct caggagcctt ctgacgttgc
      121 gaaaaacgtg cgtatgatgg tttccggtat tgtcagttac acccgctggc ctgcgctatc
      181 agggccacca aagttatgta tcttttcatc ttcacgtttt agtaccgccc tacaagagaa
      241 tgccgcgaca tctttgcctt atctgcctgt catcattcac actcaacaag aggcgatgat
      301 ttcaggctgc aatggttttt attttggcaa cgagtcaccg acattccaga tggaattaac
      361 cgaacaatat ccgtcaaagg cgttattatt aattgccgaa caaaataccg agtgcattat
      421 tggtagcgcc ttttgtctga tcatccacaa taatgacgtc agatttgccg taaacctgga
      481 tgccttatcg cgtagcggtg taaaggtcaa cccggatgtc ttaatgctcg cacggaagaa
      541 aaatgatgga taacgataat tctcttaata agcgccccac gtttaaaaga gcattacgca
      601 acatcagtat caccagcata tttatcacta tgatgctgat ctggttgctg ctttccgtga
      661 cctcggtgct gaccctgaaa cagtacgcgc aaaaaaacct ggcactgaca gcagcaacaa
      721 tgacttacag tctggaagca gctgtcgttt ttgccgatgg ccctgcagca actgaaacac
      781 tggcagcgct gggccagcaa gggcaatttt caactgcaga agtacgtgat aagcagcaaa
      841 atattctggc gtcctggcat tacacccgta aggatccagg cgatactttc agcaatttca
      901 taagccactg gctcttcccc gcccccatca ttcagccgat tcgtcacaat ggtgaaacca
      961 ttggcgaagt acgcttaacc gctcgcgaca gttcaatcag ccattttatc tggttttcgc
     1021 tcgccgtact gaccggttgt attctgctgg catcaggcat cgcaattacc ctcacccgcc
     1081 atttgcacaa tggcctggtg gaagcactga aaaatatcac cgatgtcgta catgatgtgc
     1141 gttccaaccg caatttttcc cgacgagttt cggaagaacg tatcgctgag tttcaccgct
     1201 tcgctctcga cttcaacagt ctgctggatg aaatggaaga gtggcagctt cgtttacagg
     1261 ctaaaaatgc gcagcttcta cgtaccgcgc tacatgaccc attaaccggg ctggctaacc
     1321 gcgcagcgtt tcgtagcggc atcaacacgt tgatgaacaa ttccgatgcc cgaaaaacgt
     1381 cggcgttact atttcttgat ggcgataatt tcaaatacat caatgatacc tggggtcatg
     1441 cgacgggcga tagagtcttg attgaaatcg caaaacggtt agctgaagtt ggcgggctgc
     1501 gacataaagc ataccgcctg ggcggcgatg aattcgctat ggtgctctat gatgtacagt
     1561 cagaatctga agtgcagcag atatgctcag cactgacaca aatctttaat ctcccgtttg
     1621 atcttcataa tggtcatcag accaccatga cattaagcat tggttacgcg atgaccattg
     1681 agcacgcctc tgcggaaaaa ttacaagagc ttgccgatca caatatgtat caggccaaac
     1741 accagcgtgc cgaaaagctg gtgagataac aaggatatat cgatatgata aagcacctgg
     1801 tagcacccct gattttcacc tcactaatac tgactggctg ccagtcccct cagggaaagt
     1861 ttactcctga gcaagtcgcc gctatgcaat cttatggatt tactgaatcc gccggcgact
     1921 ggtcgctggg cttatcagat gccattctgt tcgcaaaaaa tgactacaaa ttgctcccgg
     1981 aaagccagca acagatccaa accatggcag ccaaattggc ctcgacaggg ctaacacatg
     2041 cccgtatgga tggacacacc gataactatg gtgaagacag ttacaacgaa ggcttatcat
     2101 tgaaacgggc gaatgtcgtg gccgatgcat gggctatggg tggacaaatt ccacgcagca
     2161 atctcaccac acagggttta ggaaaaaaat atcccatagc cagtaacaag accgcccagg
     2221 gccgcgccga gaaccgccgc gtcgcagtgg tgattactac cccttaaaac accagcaaac
     2281 agataaaaaa gggccagcca attggccagc ccttcttaac aggatgtcgc ttaagcgaaa
     2341 tcttagttaa gacgctcttt gatacgagca gccttaccag tacgctcacg caggtagtac
     2401 agtttagctt tacgaacagc accacgacgt ttgacagaaa tgctgtcaac taccggagag
     2461 tgagtctgga agacacgctc aacgccttcg ccgttggaaa ttttacgaac agtgaatgca
     2521 gagtgcagac cgcggttacg aatagcgata accacgccct cgaatgcctg cagacgtttt
     2581 ttggaacctt caacaaccca tactttcact tccacggtat cacccggacg gaaggaaggt
     2641 acgtcctgct tcatctgctc ttgttcaagt tgcttaataa tgttgctcat aatttaatct
     2701 cttatcctgg gtaaactgat atctcggggg cttacgccat cccatcatgt ttatgttgct
     2761 gttgtgcgtg ttccgttttg aactccgcca gcaaccttgc ttgctcttca gtcagagcca
     2821 ggttttccag aagttcaggt cttctaagcc aggtacggcc cagcgactgt ttcaaacgcc
     2881 agcgacgtat ctcggcatgg ttgcccgaca gtaacactgg cggaacttcc atcccttcta
     2941 acacctcagg ccgcgtatag tgcgggcaat ccagcaatcc ttcagcaaag gaatcttccg
     3001 ttgccgaggc ttcatgtccc agtactcccg gaataaaccg ggaaacggag tcaatcagcg
     3061 tcattgctgg taactcacca ccactgagaa cgtaatcgcc gattgaccat tcttcgtcaa
     3121 tttcggtttg gatcacgcgc tcatctatac cttcgtagcg accgcacacc agaatcaatt
     3181 tttgattcgt tgccagttcg ctgacgcccg cttgatcaag cttgcgtccc tgtggtgaca
     3241 gataaatcac ctttgcgcct tcacccgccg cggcttttgc tgcatgaatg gcgtcccgca
     3301 agggttgcac catcattaac atccccggtc cgccgccgta aggacgatcg tccacggtac
     3361 ggtgccggtc atgcgtgaag tcgcgaggac tccagctctg gatgctcagc aggccatttt
     3421 taactgcccg gccagttacc ccgtaatcgg taattgcgcg gaacatttca ggaaacaggc
     3481 taattatgcc aatccacata gcgccgtctt ttaccgttta tccggtggtt taaaaaccag
     3541 gatcccaatc tacttcgatt gaacgagtag tgagatcgac tttcttgata acctgcccat
     3601 cgaggaacgg tacgagacgt tccttgatac caaacgcatc tttcaggttt gccttaatga
     3661 cgagaacgtc attagatccg gtttccatca tatcgacgac tttaccgaga tcgtagcctt
     3721 cagtggttac tacctggcag cccatcaggt ctttccagta gtagtccccc tcttcaagct
     3781 gaggcagctg cgatgaatcc acgacaattt cacaattcgt cagcaggttc gccgcatcac
     3841 gatcgtcaac gcctttcagc ttgatgatca tgtcctgatt gtggtgcttc cagctttcca
     3901 gctggacttg ctgccactga cccgccttct ggataaacca gggctgatag tcaaaaatgc
     3961 tttcggcgtc ttcggtggaa gaaaacactc tgagccaccc acgaataccg taagacgaac
     4021 ccatttttcc caaaacgatg ggatcaacag gtgcttgcgc ggtgagttgt ttgctcatca
     4081 tgaccaccgt gacagattaa gctgctttgt ttacttcttt gatcagcgca gcaacgcgat
     4141 cagaaatagt tgcgccctgg ccaacccagt gagcgatgcg atccagatcc aggcgagtgc
     4201 cttcttcttt ttcgctagcg attgggttga agaaaccaac gcgctcgatg aagcgaccgt
     4261 tgcgtgcatt acggctgtca gcgacaacaa cctggtagaa cggacgcttt ttagcgccgt
     4321 gacgtgctaa acgaatagtt accataacat cctcttgtgt gaataaaaca accggacccc
     4381 atcgaggaac ggagtccggt gtcatattaa aagcccgaaa attttactga tttctggaga
     4441 aaatgcaatc agcagttaat aactctgtcg cataaggtca tttagcgacc aggaaatccc
     4501 ggcggcatca tccccttcat gcttctcatc atcttcgcca ttccgccctt cttcattttc
     4561 ttcatcatgc gctgcatgtc gtcgaactgc ttcagaaggc ggttaacgtc ctgcacctgc
     4621 ataccgcaac cggcagcgat acgacgctta cgcgaacctt tgatgatttc tggcttagcg
     4681 cgctctttca tcgtcatcga gttgatgatg gcttccatac gcaccagcac tttatcgtcc
     4741 atctgcgact taacattatc cgggatctgc cccatgcccg gcagcttgcc catcaggcta
     4801 gccatgccgc ccatattttt catctggcgc agttgctcaa gaaaatcgtt gagatcgaag
     4861 ccgtcgcctt ttttcagctt gctggctaat ttctctgcct gcgcgcggtc aactttgctt
     4921 tcgatatctt cgatcagcga cagtacgtcg cccatgccga gaatacgcga cgcgatgcgg
     4981 tccggatgga acggctccag cgcctcagtt ttctcgccaa caccgagaaa cttgatcggt
     5041 ttgccagtga tgtgacgaat agagagcgcc gcaccgccgc gggcatcgcc gtccactttg
     5101 gtcaacacta cgccggtaag cggtaacgct tcattgaatg cttttgccgt attggccgca
     5161 tcctgaccgg tcatggcgtc aaccacaaac agggtttcaa ccgggttaat cgacgcatgg
     5221 acttgtttga tctcgtccat catcgcttcg tcaacgtgca gacgaccagc ggtatccacc
     5281 agcagcacgt cgtagaattt cagtttggct tctttcagcg ccgcgttaac gatatctacc
     5341 ggcttctgac caacatcaga agggaagaaa tcaacgccca cctgctctgc cagcgtctca
     5401 agctgtttga ttgccgccgg gcgataaacg tcggcagaaa ccaccagcac tttcttcttg
     5461 tgcttctcgc gcaggaactt accgagttta ccaacgctgg ttgttttacc ggcaccttgc
     5521 aggcccgcca tcagcacgac cgcaggcggt tgcgccgcca ggttcagggt ctggttctct
     5581 tcgcccatcg ccgcaaccag ttcgttacgg acgattttga cgaactcctg ccccggcgtc
     5641 aggctcttat taacttcatg accaaccgct ttctctttta cgcgattgat aaactcacgc
     5701 actaccggca gagcgacgtc cgcctccagc agcgccatgc gcacttcgcg cagcgtatct
     5761 tttacgttgt cttcagtgag gcgtccacgg ccactgatat tgcgcagcgt gcgcgacaaa
     5821 cgatcggtta aattatcaaa cattgtctct cgcctggggt ggaaacggtt ggccgcaatc
     5881 gcgacacatc atcagtattt tgccgcagta taacatgaag gcgtctttgt tgttatgcaa
     5941 cggttggagc agcgttcacc tgacgctata ctgcttctct ttcttattgc tcaaactgtc
     6001 gacattacta tgcccgtttt tgctctgctc gcgcttgtcg cctactccgt cagtcttgcg
     6061 ctgattgttc ccggtctgct gcaaaaaaac ggcggctggc ggcgcatggc tattatttct
     6121 gcggtcattg cgctggtctg ccacgcaatc gctctggaag cccgcatcct gcccgacggt
     6181 gatagcggac aaaacctcag cctgctgaac gttggttcat tggtcagttt gatgatctgt
     6241 acggtaatga ccattgtggc ttctcgcaat cgtggctggt tgctgctacc cattgtctat
     6301 gcctttgcgc ttatcaacct ggcgctggcg accttcatgc ccaatgaata catcacccat
     6361 ctggaagcaa cgcctgggat gctggtgcac attggcttat cgctcttttc ctatgccacg
     6421 ctaattatcg ccgccctgta cgcgctgcaa ctggcgtgga ttgattacca actgaagaac
     6481 aagaagctgg catttaacca ggaaatgccg ccattgatga gtatcgagcg taaaatgttc
     6541 cacatcacgc agattggcgt ggtgctgcta acgctcacgc tttgcactgc cctgttctac
     6601 atgcacaacc tatttagcat ggaaaatatc gacaaggctg tgctctctat cgtggcgtgg
     6661 tttgtctata ttgtgctgct gtggggacat taccatgaag gatggcgtgg acgccgcgtc
     6721 gtctggttta acgttgcggg cgcggtaatt ctgacactgg cctacttcgg cagccgaatt
     6781 gtccagcagt taatcagcta aacccagaaa aggagtttcc cctggaacac atttctacta
     6841 ctacgttgat cattattctg atcatcatgg tggtcatttc agcctatttt tccgggtccg
     6901 aaaccggaat gatgaccctc aaccgctatc gtctgcgaca tatggcgaaa cagggtaatc
     6961 gctcggccaa acgcgtcgaa aaattgctgc gtaagccaga ccgcctgata agcctggtgt
     7021 taatcggcaa taacctggtc aatattcttg cctccgcgct cggcactatt gttgggatgc
     7081 gtttgtacgg cgatgcgggc gtggcaattg cgactggtgt gctgactttt gtcgtactgg
     7141 tatttgctga ggtattgccg aaaaccattg ccgcgctgta cccggaaaaa gtcgcttatc
     7201 cgagtagttt tctgctggct ccgctgcaaa ttttgatgat gccgctggtc tggttgctga
     7261 atgctatcac ccgtatgctg atgcgcatga tgggtatcaa aaccgatatc gtggttagcg
     7321 gctctttgag caaagaagag ttgcgcacta tcgtgcacga atcacgctca caaatttccc
     7381 gtcgcaatca ggatatgctg ctgtcggtgc tcgatctgga aaaaatgacc gttgatgaca
     7441 tcatggtgcc gcgcagtgaa attatcggta ttgatatcaa cgatgactgg aaatcgattc
     7501 tgcgccaact ctcccactca cctcacgggc gcatcgtgct ctaccgtgat tcgctggacg
     7561 acgccatcag tatgctgcga gtacgtgaag cctggcggtt gatgtcggag aaaaaagagt
     7621 tcaccaaaga aaccatgctg cgcgccgcgg acgagatcta ttttgtgccg gaaggtacgc
     7681 cgctcagcac gcagttggta aagtttcagc gcaacaaaaa gaaagtcggc ctggtcgtca
     7741 acgagtatgg agacattcag gggctggtga cggttgaaga tattctggaa gagattgtcg
     7801 gcgatttcac tacgtcgatg tcgccaacac ttgccgaaga ggtcacgccg caaaacgacg
     7861 gttcggtgat tatcgatggc accgccaacg tgcgggaaat caacaaagcc tttaactggc
     7921 atctaccgga agatgatgcc cgcacggtta atggcgtcat tcttgaggca ctggaagaga
     7981 tccctgtcgc aggcacccgc gtgcgtattg gcgagtacga tatagatatt ctcgacgtac
     8041 aggacaatat gattaagcag gtaaaagttt ttcctgtgaa accgctgcgc gagagtgtgg
     8101 cggagtaacg aaaacggccc tgcattcgca tgcagggccg tgaattatta cgaaaacaga
     8161 aattacgctt ttgctttcgc tacagttacc atcgccgcac gaatcgtacg accattcagc
     8221 gtataaccct tctgcataat gcccagtacg ttacctggcg caacgtcatc agattccacc
     8281 attgcgatag cctgatgcac attcgggtcc agcggcacgt tagtttcggc gatcacttca
     8341 acaccaaact tacgcacaac atccagcatc gacttcagcg tcagctcaat gccttcaacc
     8401 atcgcggaca tatccgggtt agctttatca gccacttcca gcgcacgatc caggctatca
     8461 atcaccggca gcaattcgtt gatgaatttc tccagcgcga atttgtgggc tttttcaata
     8521 tccagttcag tacgtcgacg caggttttcc atttcggctt ttacacgcaa aatgccgtca
     8581 cgttcacggg tctgggcttc agccagctga gcttcgagat tcgcaatttt ttcatcgcgc
     8641 gggtccacct gctcagcaga agcttctggc tcaactgcct caatctcttc gtgctgatcc
     8701 atgataattt cttccggggc ttgcccctca ggcgttttct gttctttact actcatgaat
     8761 ttctccgcgt ttttttcgca ttcatctcgc taacttcgct tattatgggg atcagtttca
     8821 gggtttcaag ggaagcactc acattgtcat caatcttcgc aacaaggacc tcggaaaaat
     8881 gaataatcat ttcaagtgta ttggcattgt gggacaccca cggcacccca ctgcactgac
     8941 aacacatgaa atgctctacc gctggctgtg cacaaaaggt tacgaggtca tcgttgagca
     9001 acaaatcgct cacgaactgc aactgaagaa tgtgaaaact ggcacgctcg cggagattgg
     9061 gcaactagct gatctcgcgg tagtcgttgg tggcgacggt aatatgctgg gcgcggcacg
     9121 cacactcgcc cgttacgata ttaaagttat tggaatcaac cgtggcaacc tgggtttcct
     9181 gactgacctt gaccccgata acgcccagca acagttagcc gatgtgctgg aaggccacta
     9241 catcagcgag aaacgttttt tgctggaagc gcaagtctgt cagcaagatt gccagaaacg
     9301 catcagcacc gcgataaatg aagtggtgct tcatccaggc aaagtggcgc atatgattga
     9361 gttcgaagtg tatatcgacg agatctttgc gttttctcag cgatctgatg gactaattat
     9421 ttcgacgcca acaggctcca ccgcctattc cctctctgca ggcggtccta ttctgacccc
     9481 ctctctggat gcgattaccc tggtgcccat gttcccgcat acgttgtcag cacgaccact
     9541 ggtcataaac agcagcagca cgatccgtct gcgtttttcg catcgccgta acgacctgga
     9601 aatcagttgc gacagccaga tagcactgcc gattcaggaa ggtgaagatg tcctgattcg
     9661 tcgctgtgat taccatctga atctgattca tccgaaagat tacagttatt tcaacacatt
     9721 aagcaccaag ctcggctggt caaaaaaatt attctaattt tacgccagcc tctttactgt
     9781 atataaaacc agtttatact gtacacaata acagtaatgg tttttcatac aggaaaacga
     9841 ctatgttggc acaactgacc atcagcaact ttgctatcgt tcgtgagctt gagattgatt
     9901 ttcatagcgg catgaccgta ataactggcg agaccggcgc gggtaaatct attgcaatag
     9961 atgccctcgg tctttgtctc ggtggtcgcg ctgaagccga catggtgcgt accggcgctg
    10021 ctcgcgctga cctgtgcgcc cgtttttctc tgaaagatac gccagcggct ctgcgctggc
    10081 tggaagaaaa ccagcttgaa gacgggcatg aatgtttgct tcgtcgcgtg atcagcagcg
    10141 atggtcgctc ccgtggtttc atcaacggta cagctgttcc tctgtcacaa ctgcgcgaac
    10201 tgggtcagtt gctgattcag atccatggtc agcacgctca tcagttactc accaaacccg
    10261 agcaccaaaa gttcctgctt gatggctatg ccaatgaaac ctctctactc caggaaatga
    10321 cagcacgtta tcagttgtgg catcaaagct gccgtgacct cgcgcatcat caacagttaa
    10381 gtcaggaacg cgctgcccgt gcggaactgc tgcaatacca attaaaagaa cttaacgaat
    10441 ttaatccgca gcccggagag tttgaacaaa tcgacgaaga gtacaaacgt ctggcgaaca
    10501 gcggtcaatt gctgaccacc agccagaatg cattggcatt aatggccgac ggtgaagacg
    10561 caaacctgca aagtcagctt tacacggcta aacaactggt gagcgaattg attggcatgg
    10621 acagcaaact gtccggcgta cttgatatgc tggaagaagc taccatccag attgctgaag
    10681 ccagcgatga actgcgccac tactgcgatc gtctggatct cgatcccaac cgactatttg
    10741 aacttgaaca gcgcatctca aaacagattt cgctggcacg taaacatcac gtcagccctg
    10801 aggcattgcc acagtattac cagtcgctac tggaagaaca gcagcaactg gacgatcagg
    10861 ccgactcaca agaaacgctt gcgctggcgg taacgaaaca tcatcagcag gcactggaaa
    10921 tcgcgcgcgc attacaccaa caacgccagc aatatgcaga agaacttgca cagctgatca
    10981 ccgacagtat gcatgcgctc tcaatgccgc atgggcagtt tacgatcgat gttaaatttg
    11041 acgagcatca cctgggcgct gacggtgccg atcgtattga gtttcgggta accaccaacc
    11101 caggtcagcc aatgcagcct attgccaaag tcgcatccgg tggtgaattg tcccgcatcg
    11161 cactggcaat ccaggtcatc acggcgcgta aaatggaaac cccggcactg atttttgatg
    11221 aagtggatgt agggattagc ggtccaacag cggcagttgt cggcaaactg ctgcgtcaac
    11281 tcggcgaatc aactcaggtg atgtgtgtta cccacctgcc acaagtcgcg ggatgtggtc
    11341 atcaacacta ttttgtcagc aaagaaaccg atggtgcgat gacagaaacg catatgcaat
    11401 ccctgaataa aaaagcgcgg ttacaagagc tggcgcgcct gcttggtggc agtgaagtca
    11461 cacgtaatac actggcgaat gcgaaagaac tgcttgcagc gtaaactttt ttcctgcttc
    11521 acggtcagag taaacagcaa aacgccgtaa gaccggaaag caaaaggttt taaagtgatg
    11581 aaaggtctat tatcatcggc atattacaga tgagccacgt actgctcggg cccgaaaagg
    11641 aatcaaatca ctatgcgctg taaaacgctg actgctgcag cagcagtact attgatgttg
    11701 accgcaggct gttccactct ggagcgagtg gtttaccgtc ctgacatcaa ccaggggaac
    11761 tatctgaccg ctaacgacgt atccaaaata cgtgttggca tgacgcaaca acaagttgcg
    11821 tacgcattgg gtacaccgct gatgtccgat ccatttggta cgaatacctg gttctatgtc
    11881 ttccgccagc aaccaggtca tgaaggtgta acgcagcaaa cgctgacgct gacctttaac
    11941 agtagcggtg tgttgaccaa tattgataac aaacctgcgc tgagtggtaa ctaataataa
    12001 agttgctctc aaagacgtta aaaaaggtgc tcaatgagca ccttttttct gtctgttatt
    12061 tattcgctga tttttctgct cgttgcctgc gaagctcttt cggatcggca atgagagggc
    12121 gataaatctc cacccgatcg ccatcatgca cactatcgct tagttttgcc ggacggctgt
    12181 aaatgccgac tttgttttta gttaaatcga tatcggtacg caattccagc aagccactgg
    12241 cgcgaatagc ttcttcaacc gtcgcgccct cctgcagcgt cactcgctgc aggtactgct
    12301 tctcaggtag cgcataagcc acctcaacgg caattttacc tggcactgta aacctctttc
    12361 gcacgaaccg taaaagcctg gaccatatta gccgccagct ctttaaacac gcgaccaaag
    12421 gcgagttcaa tcaacttatt ggtaaactca aagtcgagat gaaactcgat acgacacgcc
    12481 tcctggctca gcggcgtaaa cttccatcca ccaatcaatt tcttgaacgg cccatccacc
    12541 agattcataa gaatactttg gttactggtc aactggttgc gggtagtaaa cgttttgctg
    12601 atcccagcct tagagacatc taccgccgca gtcatctgcc caggagtgga ctccagaatc
    12661 cgacttccgg tacaacccgg caaaaactga ggataagact gaacgtcatt cactaactga
    12721 tacatttgct ccgcgctgta gggtaccagt gcggtccggc taatctgagg cataacaatt
    12781 tccatcaaca aaaatccaac aaataatatc atttatcctg ctgtaaaaaa aacgctatcc
    12841 cggcgctggg taacatcggg ttcatgctaa gatagagcct tgtcccccgc aggattgata
    12901 tggggtgttt tcgatttcag attaccgatg attcacgacg cttatgacga agaaaaaagc
    12961 acataaacct ggttcagcga ccatcgcgct taacaagcgc gcccgtcacg aatactttat
    13021 cgaagaagag ttcgaagcgg gacttgccct gcaaggctgg gaggttaaat ccctgcgcgc
    13081 aggaaaagcc aatatcagcg acagctacgt ccttctgcgt gacggagagg catttctgtt
    13141 tggcgctaac atcacgccaa tggccgtggc ctccacgcat gtggtgtgcg atcctacccg
    13201 tacccgcaag ttacttctca accaacgcga actggactca ttgtacggtc gcgtcaatcg
    13261 agaaggctat accgtagtgg cgctctccct gtactggaaa aatgcctggt gcaaagtgaa
    13321 aatcggcgtc gccaaaggta aaaaacagca cgataaacgt tcagatatca aagaacgcga
    13381 atggcaggtg gataaagcac gtatcatgaa aaacgcccac cgttaaacct gcactccaat
    13441 tattgaccag ttcctcaccg cgcctccctc tccggcggcg cgaatgaaca tcttattggc
    13501 tatcacatcc gacacaaatg ttgccatccc attgcttaat cgaataaaaa tcaggctaca
    13561 tgggtgctaa atctttaacg ataacgccat tgaggctggt catggcgctc ataaatctgg
    13621 tatacttacc tttacacatt ggggctgatt ctggattcga cgggatttgc gaaacccaag
    13681 gtgcatgccg aggggcggtt ggcctcgtaa aaagccgcaa aaaatagtcg caaacgacga
    13741 aaactacgct ttagcagctt aataacctgc ttagagccct ctctccctag cctccgctct
    13801 taggacgggg atcaagagag gtcaaaccca aaagagatcg cgtggaagcc ctgcctgggg
    13861 ttgaagcgtt aaaacttaat caggctagtt tgttagtggc gtgtccgtcc gcagctggca
    13921 agcgaatgta aagactgact aagcatgtag taccgaggat gtaggaattt cggacgcggg
    13981 ttcaactccc gccagctcca ccaaataatg atccggatac gtccggtgaa gtacagaaag
    14041 cccgcatggc acaagacctg cgggcttttt tgtgtctgtt gttgtccgag aacatccggc
    14101 taaatccggt gattattggt atacgtttag gtatacggta ggatgtatac ctaaaagcgt
    14161 ataccaattc atgaaggagc ggccacagtg gcacggacaa cacgccccct gacaaacacc
    14221 gaagttctgc gcgcaaaagc gttagagaag gatctaacgc tgcatgatgg cgatgggctt
    14281 ttcctgatag tgaaaaccag cgggaaaaag ctctggcgtt tccgttatca acgtccgaca
    14341 acaaagcagc ggacaatgat ggggctcggt gccttccctg ccctttcgct tgctgatgca
    14401 cgaaggttaa gagcggatta ccttgcccta ttagccaacg gaatcgaccc acagattcaa
    14461 gctgaagttg cagaggaaga gcagcaaatt gctctggaca gtattttttc tactgttgcc
    14521 gctaactggt tccaactcaa aagcaaaagc gttacccctg attacgcaaa agacatttgg
    14581 cgctcactgg agaaagatgt atttcctgcc attggtgaga tccccgttca gcaaatcaaa
    14641 gcccgaacat tggttgaagc acttgagcca atcaaagccc gtggagcgct tgagactgtt
    14701 cgtcgactgg tgcaacgcat aaacgagata atgatttatg cggttaacac cggtttgatt
    14761 gatgccaatc ctgcatctgg tgtagggatg gcttttgaga agcccaaaaa gaaaaatatg
    14821 ccgacattgc gaccagaaga attaccgaag ctgatgcgtt ctttagtcat gtcaaatctg
    14881 tctgttccga ctcgctgtct aatcgaatgg caactcctga cccttgtgcg cccttctgag
    14941 gcctccggta ctcggtgggc agagatagat ctcgaggcaa aactctggac aattccagcc
    15001 gaaagaatga aagccaaacg tgaacacata gtacctctat catctcaagc attaaatatt
    15061 cttgaagtga tgaaacctat cagtgctcac cgagaatata tattccccag caggaacgat
    15121 ccgaaacaac ctatgaacag tcaaactgct aatgctgcac taaagcgaat cgggtatgcg
    15181 gggaaacttg ttgcgcacgg aatgcgttca atagccagca ccgcgatgaa tgaaacagga
    15241 tttaattctg acgtcattga gtctgcttta gctcacattg agaaaaatga agtaaggcgt
    15301 gcctacaata gagcattata tttggaacaa aggaaagagt taatgcagtg gtggggtaac
    15361 tttataaaga aagccatgtg agcaacatgg ctttcactga tgcttaaatc aaatcacatc
    15421 caaaatctcg ctaaatttat ttatagagta ggtagggatg acatgagagt ataactcttt
    15481 gagtttgctc tctctctgaa catcatctgc actccaatga gtaatcgaaa ctaatacatt
    15541 ccaataatca ggattatgtt gtttcccata taatgcaaga attgactcaa taccagcact
    15601 attagccata gatatatctt tgattattga atcgccgaca taaatagcat cttttaaatc
    15661 aacacactcc cgataacaaa tatcacccaa caa
//
LOCUS       DBANUU010000097        15512 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000097 DBANUU010000000
VERSION     DBANUU010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.097"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            34..600
                     /gene="yceJ"
                     /locus_tag="ACIE68_003244"
     CDS             34..600
                     /gene="yceJ"
                     /locus_tag="ACIE68_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFX5909516.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            604..1179
                     /gene="yceI"
                     /locus_tag="ACIE68_003245"
     CDS             604..1179
                     /gene="yceI"
                     /locus_tag="ACIE68_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFX5909517.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1221..2273)
                     /gene="trhO"
                     /locus_tag="ACIE68_003246"
     CDS             complement(1221..2273)
                     /gene="trhO"
                     /locus_tag="ACIE68_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFX5909518.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2498..3418
                     /gene="lpxL"
                     /locus_tag="ACIE68_003247"
     CDS             2498..3418
                     /gene="lpxL"
                     /locus_tag="ACIE68_003247"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFX5909519.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3590..4816
                     /gene="mdtG"
                     /locus_tag="ACIE68_003248"
     CDS             3590..4816
                     /gene="mdtG"
                     /locus_tag="ACIE68_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFX5909520.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4899..5273
                     /gene="msyB"
                     /locus_tag="ACIE68_003249"
     CDS             4899..5273
                     /gene="msyB"
                     /locus_tag="ACIE68_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFX5909521.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5274..5501)
                     /gene="yceK"
                     /locus_tag="ACIE68_003250"
     CDS             complement(5274..5501)
                     /gene="yceK"
                     /locus_tag="ACIE68_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFX5909522.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5674..8217)
                     /gene="mdoH"
                     /locus_tag="ACIE68_003251"
     CDS             complement(5674..8217)
                     /gene="mdoH"
                     /locus_tag="ACIE68_003251"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFX5909523.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8210..9745)
                     /gene="mdoG"
                     /locus_tag="ACIE68_003252"
     CDS             complement(8210..9745)
                     /gene="mdoG"
                     /locus_tag="ACIE68_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFX5909524.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10139..11296
                     /gene="mdoC"
                     /locus_tag="ACIE68_003253"
     CDS             10139..11296
                     /gene="mdoC"
                     /locus_tag="ACIE68_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFX5909525.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11304..12785)
                     /gene="clsC"
                     /locus_tag="ACIE68_003254"
     CDS             complement(11304..12785)
                     /gene="clsC"
                     /locus_tag="ACIE68_003254"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFX5909526.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12727..13260)
                     /gene="ymdB"
                     /locus_tag="ACIE68_003255"
     CDS             complement(12727..13260)
                     /gene="ymdB"
                     /locus_tag="ACIE68_003255"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFX5909527.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13355..13666)
                     /gene="ymdA"
                     /locus_tag="ACIE68_003256"
     CDS             complement(13355..13666)
                     /gene="ymdA"
                     /locus_tag="ACIE68_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFX5909528.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13787..14119)
                     /gene="csgC"
                     /locus_tag="ACIE68_003257"
     CDS             complement(13787..14119)
                     /gene="csgC"
                     /locus_tag="ACIE68_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFX5909529.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14178..14633)
                     /gene="csgA"
                     /locus_tag="ACIE68_003258"
     CDS             complement(14178..14633)
                     /gene="csgA"
                     /locus_tag="ACIE68_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFX5909530.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14674..15129)
                     /gene="csgB"
                     /locus_tag="ACIE68_003259"
     CDS             complement(14674..15129)
                     /gene="csgB"
                     /locus_tag="ACIE68_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFX5909531.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3861 a         3980 c         3691 g         3980 t
ORIGIN      
        1 tctttctctc gcaaattcgt attggatgca cttatgtcat tcacaaatac ccctgaacgc
       61 tatggtgtta tatctgcggc cttccattgg ctaagcgcaa ttattgtcta cggcatgttt
      121 gctttaggtc tgtggatggt cacgctcagt tattacgatg gctggtatca caaagcaccc
      181 gaactgcata aaagcatcgg tattttgtta atgatggggc tggttattcg cgttctgtgg
      241 cgtgtcatat ctccaccgcc gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc
      301 gcaaaggctg gacatctcgc attgtaccta ttgctgttcg ccatcggcat cagcggctat
      361 ctgatctcaa ctgccgatgg taaaccgatc agcgtttttg gctggtttga cgtccccgcg
      421 acccttgccg acgctggcgc acaggcagac tttgccggtg ccctgcattt ttggctcgcg
      481 tggagcgtcg tagtactgtc cgttatgcac ggatttatgg ccctgaagca tcatttcatc
      541 gataaagacg acactctgaa gcgcatgctg ggaaagtcgt catctgacta tggagtataa
      601 aaaatgaaaa aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc tgccggttca
      661 gcggttgccg ccgattacaa aattgacaaa gaaggtcagc acgcctttgt taatttccgc
      721 atccagcacc ttggctatag ctggttatac ggcaccttta aagatttcga cggtactttt
      781 acctttgacg aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa caccaccagc
      841 gtcgatacta atcacgccga acgcgataaa catcttcgca gtgcagattt cctcaatacc
      901 gcaaaatatc cacaggcaac attcacctcc accagcgtga agaaagacgg tgacgaactg
      961 gatattaccg gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa
     1021 ttaattggtc agggcgacga cccatggggt ggtaaacgtg caggcttcga ggccgaaggc
     1081 aaaattaagc tcaaggactt caatatcaag acagatttag gtccagcttc tcaggaagtg
     1141 gatctgatta tttcagtgga aggcgtacag cagaagtaag agtgaaatgt tgccggatgg
     1201 cacgcatccg gcaatgatat ttattccgtt ggatcaggaa tgcccagtgt tgtattcaga
     1261 cgtccacgag acttattaaa gatcttattg ccattttcac gtcctgcccg acggcgtcgc
     1321 tgttcttctg gcggtaacgc gctttcttcg cagcaaatct cactacaaca acctttgtat
     1381 ttttccgcac atactggaca ctgaataaac agcaggtggc agccatcatt tttacagttg
     1441 gtatggctgt cgcacggcgc gccgcactgg tggcaatgcg cgataatctc atccgatata
     1501 cgttcgccca tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc
     1561 tgctcgcgcg ccttacgggc gtattcaata attccgccct cgatatgcca gactttgttg
     1621 aatccgttat gtttcatcca ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac
     1681 atgacgattt ttttatcttt atgtgcctgc atcatctcga ctgcttttgg cagctgctca
     1741 cggaaggtat ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc atactcatag
     1801 tggttacgca tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc
     1861 gcttgcagat actcaccaac attgctggca tcaaagtgag gatcgtcaat accgtcggca
     1921 acaatgcgat cgcgtacctt catgcgcagt acccagaaag atttcccgtc atcatccaac
     1981 gcgatattca ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat
     2041 gtttcaacat tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg cgccagatac
     2101 actcgcccaa aaacattcag cgcggtaaac agctgatata aagcgtcacg ggtcgcctta
     2161 ggatcggcga tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc gctctcagcc
     2221 aacattttgg cttttagcgc gtcgttggaa atgcggttgt gtaacactgg catggtgtac
     2281 ggttcctgcg agatgggaaa gtaaaaatcc gcggcatgat atagcaatta tcgataatta
     2341 acatccacac attttacgct acatttgcgc attaaaaatt atttgttatt tacaagcgcg
     2401 gcaatttcgc ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac
     2461 atacaattgc ccgaataggt tgaaaaacag gattgatatg acgaatctac ccaagttctc
     2521 caccgcactg cttcatccgc gttattggtt aacctggttg ggtattggcg tactttggtt
     2581 agtcgtgcaa ttgccctacc cggttatcta ccgcctcggt tgtggattag gaaaactggc
     2641 gttacgtttt atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc
     2701 ggaaatgagc gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat
     2761 gggcctgatg gaaaccggca tggcgtggtt ctggccggac cgccgaatcg cccgctggac
     2821 ggaagtgatc ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt
     2881 agttggcatc cattttctga cactggagct gggtgcgcgg cagtttggta tgcaggaacc
     2941 ggggattggc gtttaccgcc cgaacgataa tccactgatt gactggctac aaacctgggg
     3001 ccgtttgcgc tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc
     3061 cctgaaaaaa ggcgaagtgg tctggtacgc accggatcat gattacggcc cgcgctcaag
     3121 cgttttcgtc ccgttatttg ccgttgagca ggctgcgacc acgaccggaa cctggatgct
     3181 ggcacggatg tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa
     3241 agggtatcaa ttgattatgc tgccgccaga gtgttctccg ccactggatg atgccgaaac
     3301 taccgccgcg tggatgaaca aagtggtcga aaaatgcatc atgatggcac cagagcagta
     3361 tatgtggtta caccgtcgct ttaaaacacg cccggaaggc gttccttcac gctattaaat
     3421 ctcccctgcc ggatgcttca gaatggcatc cggcattacc acagcaaatc cccctgattt
     3481 agcgataaaa gctctctgga ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta
     3541 tcttttcttc ttatgttcac cgcgcctggc gcaccaacag cggattgcta tgtcaccctg
     3601 tgaaaatgac acccctataa actggaaacg aaacctgatc gtcgcctggc taggctgttt
     3661 tcttaccggt gccgccttca gtctggtaat gcccttctta cccctctacg ttgagcagct
     3721 tggcgttacc ggtcactccg ccctgaatat gtggtccggt attgtcttca gcattacatt
     3781 tttattttcg gccatcgcct caccgttttg gggtggactc gccgaccgta aaggccgaaa
     3841 actcatgcta ttacgctctg ccctcggcat gggcatcgtg atggtgttga tgggactggc
     3901 acaaaatatc tggcagtttt tgatcctacg ggcgcttctt gggttacttg gcggatttgt
     3961 ccccaacgct aatgctctta tcgccacaca agtaccgcgt aataaaagcg gctgggcgct
     4021 gggtacgctc tccacaggcg gcgttagtgg tgcgttgctc ggcccaatgg ctggcggcct
     4081 gctcgccgat agctacggct tacgtccggt attctttatt accgccagtg tgctcatact
     4141 ctgctttttc gtcaccctgt tctgcatcag agaaaaattc cagccggtca gcaaaaaaga
     4201 gatgctgcac atgcgggaag tggtgacatc acttaaaaac ccgaaactgg tactcagcct
     4261 gtttgtcacc acgttaatca tccaggtggc gacaggctca attgccccca ttctgacgct
     4321 gtatgtccgc gaactggcgg gtaacgtcag taacgtcgcc tttatcagtg gcatgatcgc
     4381 ctcggtgcca ggcgtggcgg ctctgctaag cgcaccacga ctcggcaaac ttggcgatcg
     4441 aatcggaccc gaaaagatcc tgattacagc gctgatcttt tctgtactgc tgttgatccc
     4501 aatgtcttac gttcagacgc cattgcaact tgggatttta cgttttttgc tcggtgccgc
     4561 cgatggtgca ctactccccg ccgtacagac actgttggtt tacaactcga gcaaccagat
     4621 cgccgggcgt atcttcagct ataaccaatc gtttcgtgat attggcaacg ttaccggacc
     4681 attgatggga gcagcgattt cagcgaacta cggtttcaga gcggtatttc tcgtcaccgc
     4741 tggcgtagtg ttattcaacg cagtctattc atggaacagt ctacgtcgtc gtcgaatacc
     4801 ccaggtatcg aactgatttt tcgcctttca tacttgcaaa agcggagaat cagctatcct
     4861 tttccctgaa acctcatcaa ctcaaaggga gaatcgtgat gaccatgtac gcaacgcttg
     4921 aagaagccat tgacgctgca cgcgaagaat ttcttgcaga caaccccggc atcgacgctg
     4981 aagatgcgaa tgtgcaacag ttcaatgccc aaaaatacgt tttgcaggac ggcgacatca
     5041 tgtggcaagt tgagtttttt gccgacgaag gggaagaagg tgaatgttta cctatgctta
     5101 gcggtgaagc cgcgcaaagt gtttttgatg gcgactatga tgagatagag atacgccagg
     5161 agtggcagga agagaataca ttacatgaat gggacgaggg ggaatttcag cttgagccac
     5221 cgctggatac cgaggaagga cgcgcagcag ctgatgagtg ggatgaacgt taatcactca
     5281 tacgggccat gatgaatgtc gatcggcagc agtaaagtat caaaaaccag agagaatggc
     5341 agatcaagga tcgtgacata acgccaggcg gagtcacgca catcccattg cacaccggga
     5401 taatattggt tgccatgccc ctgccccggt atagtgcgac taataatgct gccacagcca
     5461 ctcagcaagg tcaccatgat gctcaccaca attaaacgca tttgatacct ctttcgtaaa
     5521 aaaataccgg cataacgccg gtatttttat tggctcgttg tttttgccgg atgcggcgtg
     5581 aacgccttat ccgacccaca aaatcttgca aaatcaataa attgcaggaa cgatgtaggc
     5641 ctgataagcg tagcgcatca ggcaactacg tttttattgc gaagccgcat ccggtttacg
     5701 caatgccagt ggattgagct ttatcccttc gtaataactc acccatgaag aatatctctc
     5761 cggggaattc cagacgcgga aatgcagacg ggccatcgtc accggatcgc ttagcagcac
     5821 caggcgacga tcgcgattca gcttctctgg cgtctcgttc agcgcctgtt caacgtggcg
     5881 gtcacgggcg atttccagca ccttactggc gcggtgacgc gcggtcgcca ttgcggttgc
     5941 cagagcgtta aatgacgggt taaacactgc gtgcataaag ccatcatcaa gggagcgttg
     6001 acgattcatc tcaaggaacc gatcggtatc aaccagcacc tgcggcggcg aatactcttc
     6061 cgggatcagg aacagtttcc agcgtttggt gcgcagacca acggtggcac ggctggaaat
     6121 caccgaaaca aacggtgaca ggatcaacga gaagacaatc ggtgccagcc agaacaggaa
     6181 acgcagatcc aaccacgcca taccaacagc ccacactaac cctaacagca gctgtgagcc
     6241 gtggcgtttg aacgcttcac cccaggaagt ggagtcatca tcacgctgcg gtgaattcca
     6301 caccacttcc cagccaagga acgcgctgac gacgaagacc gtatggaaca gcatgcgtac
     6361 cggagccagc agcacggaaa acagcacttc cagcagcaac gataatgtaa cgcgccagaa
     6421 gccgccgtat tctttcgttc ctttgcacca gataagcaaa atgctcaata acttcggcag
     6481 gaacaacagc accatggtcg aagcaaaaag tgcaatcgcc agctcaggac gccactgcgg
     6541 ccacaccggg aacaactgcc gtggttgcag gaagtattgc ggttcggtca acgcatgtac
     6601 tacctgcaat gcagtagaga gcgcgaggaa cataaaccac agcggagcgg agagataaga
     6661 catcacgccc gtcaggaata ccgcacggtg aaccgggtgc atacccttca ccaggaacag
     6721 acggaagttc atcaggttac cgtggcacca gcggcggtca cgttttagct catcaagcaa
     6781 gttaggcggc aattcttcat aagaacccgg gagatcgtaa gcaatccaga ccccccaacc
     6841 tgcacggcgc atcaacgccg cttccacgaa gtcatgtgac aggattgaac cggcaaagga
     6901 accttcgccc ggcagcggag ccagtgcgca gtgctcgata aacggtttca cgcggataat
     6961 cgcgttatgt ccccagtagt gcgactcgcc aagttgccag aagtgcaaac cggctgtaaa
     7021 cagtggccca tacacgcggg tcgcgaactg ctgacagcgc gcatacagcg tatccatacc
     7081 ggacgctttc ggcgacgact gaatgatccc ggcgttcggg ttggcttcca tcaggcgcac
     7141 cagcccgcac aaacaatcac cggtcattac cgagtcagca tccagcacca ccatgtagct
     7201 gtactggctg ccccagcgac ggcagaagtc atcgatatta ccgcttttac gcttcacgcg
     7261 gcgacggcgg cggcgataga aaatctgacc ttcgccaccg acttcagcga taagctccat
     7321 ccaggctttt tgctctgcga cgcagatatc cgggttataa ctgtcactaa gaatgtagac
     7381 atcaaagtgt ttggcattcc cggtggcttt tactgattcc cacgttgcac gcaggccagc
     7441 aaaaacacgg ttcacgtctt cgttacagat aggcatgatc aacgccgtgc gatgctccgg
     7501 gtttaatggt tcatcgccaa ctgttgacgc agatatactg tatttatcgc gaccaataag
     7561 cagttgcagg aagcccatta acgccgtcca gaatccggcg gacacccaac agaacagtac
     7621 cgcaaagagg atcaggatac cggtttgcag catataaggc agaagctgca taaaggaaac
     7681 ccacaaatcc tgaccaacca tatccatagg attaatcagc gcccaaccct gataaggaag
     7741 aatggtcttc atataccagg tcgcgacgac agtttgcgcg agcgtcagga tcaacagaat
     7801 gtaacggcgg atggtaccga cggtacgcca cttttgctcg ctctcctgct cttctttggt
     7861 caaacgagcc agatagcgcg gcgtgacatc gcgtccacgc aggcgatccc agaaacggcc
     7921 taccgggttg gtacgccacg ggtcgggaaa catcgaggag cgttttgctt ctggcatcgc
     7981 cttcagctga tcgcgccctt cgtcgtcttt aattaactgt ccatcagcaa gtgaatctgg
     8041 ccaggcttgt tccagacgcg cctttaccga gccttgcggg gaatcatcct cccgcgccca
     8101 ggtgcggtgt tcggcatcca gcgcctgatg aacggcgcgg atatcagtct tcggcaatgc
     8161 cgctttctcg cttgcggcga tgggcattgc gtcaatgtac tcagttgtct tattcattgg
     8221 caggtaactg gtagctccag gtttcactca acgtctgatc ggcattcacc agcgcagcac
     8281 gcatttcagt ggttttcttg gcatctttca ctttcacacg catcaccaga cgccagcctt
     8341 tggtaaccgg gttatagcgc accgtgcttt caactatctc accattatca ccaatgctgg
     8401 tttgcgctgt gaccggggta tcctctggca gttttttcat ctctgcgccg gtaaaatcga
     8461 ccacaaaggc gatagtaccg tcaggctggc gaatcaggtt cgactgcttc acatcccccg
     8521 ttgaacgacg cgtttgttgc acccatgcgt tatctggcgc atgcagtttg tcttcatcac
     8581 ggctgaaggt gatggtgtat ttaaagttca tctctttacc cggctccggc agctgatccg
     8641 gcgtccagta agcgacgatg ttatcgttgg tttcatcgtt ggttggaatt tccaccagct
     8701 caacgctgcc tttaccccac tcccctttcg gagtcaccca tgcgcttgga cgaagatcgt
     8761 aacgatcatc gagatcttca aagcgggaga aatcacgacc gcgctgcaac agaccgaagc
     8821 cttgcgggtt ctccatcgag aagctgctga ccgctaaatg tttcgggtta ttcaacggac
     8881 gccagatcca ttcgccatta ccggcatgga tagacagacc gttagagtcg tgcaactccg
     8941 gacgatagtt atttgcaggc gacggttggt tcggcccaaa caggaacata ctggttaacg
     9001 gtgcaacccc cagtttgccg actttatcgc gcagatagat tttcgactgc acatccacaa
     9061 ccgtgtcacg ccctggcata accacgaatt tgtaagcacc tgtcgcgcgc ggcgagtcaa
     9121 gcaatgcata aatggttaaa cgtttatcag tcggttttgg acgctcgatc cagaactctt
     9181 tgaagcgtgg aaattcttca cccgatggca aggcggtatc aattgccagg ccgcgggcag
     9241 aaaggccata aacctgacct gcaccaatca cgcggaaata gctggccccg agcatgctga
     9301 cgatttcatc gtttttatct ttgctgttga tcgggtaaag cactttaaaa ccggcaaaac
     9361 caaggtcttt taccgtgtct ttgtcatgct gaacatcgcc gaaagtgaaa taatccgggc
     9421 tgtatttgat tcgtttgact gcggtggcag tcacttcatt tattttgacc ggggtatcga
     9481 agtacatacc ctgatggtag aactcgagtt tgaatggggt cttcagattg ttccagtacg
     9541 ctttgtcatg attaaactgg atctgctgat agtccgcgta tttcatatcg cggaaaacgg
     9601 agggcaagtt gcttttgggc gcctcatagc ctttcccggc taaggattga gcttgctttg
     9661 cgacatcatc aatactgaaa gcccagcttg aagatgtata cagggttaac attactgcag
     9721 cactcaacca acgcattttc atcatttgta gtttatgttt cataattagt aagcacttcc
     9781 ccctttgtgt gcttatatcg atccgatcta ttttaatgga taaccagcca ttccgacaac
     9841 agaatcccgg tatcgttcag cacgctggct catggattaa gcacctgatt gcaaccgttt
     9901 tcactttccg tgcttctcct aaagacagaa gcatcgactt cgtgtagggt ttgccagcaa
     9961 ttttatttat caaccgtctt acagacgctt ccattctggt tagtggtagc gatgatgcta
    10021 tgcataaaaa ccttgaagcc tgcgcctggc gcggtcaggt cggtgtcgct atggcaacaa
    10081 gcctaaaggc ataacccgac agggataaga cgaaaaatcc gagattagtt aaccatatat
    10141 gaatccagta cccgcgcaac gtgaatattt cctcgactcc atccgcgcct ggctgatgtt
    10201 gttagggatc ccttttcata tttctttaat ctattcgagc catacatggc atgtgaatag
    10261 cgccgaacca tcattgtggc tgaccctttt taatgacttc atccactcgt tccgcatgca
    10321 ggtatttttc gttatatccg gctacttttc ctacatgctt tttttacgct atcccttgaa
    10381 aaaatggtgg aaagtacgtg tcgaacgtgt aggtatcccg atgttaacag ccatccccct
    10441 actgacatta ccgcaattta ttatgctgca atatgtcaaa ggaaaagcgg aaagttggcc
    10501 tgggctgtca ttgtatgaca aatataatac gttggcctgg gaattaatat cacacctgtg
    10561 gtttttactg gtgttagtag tcatgacgac gctgtgcgta tggatattta agcgcatcag
    10621 aaataattta gaaaattctg ataaaacgaa taaaaaattc tcgatggtaa aactatcggt
    10681 gattttttta tgcctcggca tcggttatgc ggtaataaga agaacgattt ttattgtgta
    10741 tccgcccatt ctgagtaatg gcatgttcaa ttttattgtc atgcaaacgc tgttttattt
    10801 gccgttcttt atcctcggcg cactggcttt cattttccct catcttaaag ccttgtttac
    10861 cacgccgtct cgtggctgta cccttgcagc agcattggcg tttgtcgctt atttactcaa
    10921 ccagcgctat ggcagtggcg atgcctggat gtacgaaacc gagtcggtga tcaccatggt
    10981 cctcggtctg tggatggtga atgtggtctt ctcctttggc caccgtttgc ttaacttcca
    11041 gtcagcgcgg gtgacttatt ttgttaacgc atcgctgttt atctatctgg ttcaccaccc
    11101 gttaacgctg tttttcggcg catacattac accgcacatc acctccaact ggcttggttt
    11161 tctctgtggc ctgatatttg tagtagggat tgcgataatt ctgtatgaaa ttcatttgcg
    11221 catcccgtta ctgaagtttt tgttctctgg taaaccggtt gttaagcgtg agaacgataa
    11281 agcaccagcc cgttaagcca catttacaat aaccattcca cgggcaatat cgacgccagt
    11341 ctgaccataa cccgcttcca gaaactggtg gcgggttctt ttttgagaat aatctctttc
    11401 tttgcatgac gatcaaccca gttgatccgt ccccacctgt ccagacggag ctgccaggcc
    11461 gcatcatact ggctctgaat aaagcgttta tcaattaact gtgccagcgt ttcgctctct
    11521 atcacgaagc ccatttcagt attgagcaat gttgaacgcg gatcgaaatt gaaagagccg
    11581 ataaacaccg ttttaccatc gatgctaaag gttttagcat gcaggctggc tccggaatta
    11641 ccggttatgc cgcgatcgtg taacgtacta ctttgttcac gcgtcggctt gagttcatat
    11701 aattccacgc catagcggag caattttttg cgccagcgcg cgtatccggc atggacgaca
    11761 gcaacatcgt tagcggcaag agaattggtt aggatcgcaa tctttacccc ttttctcacc
    11821 atccgtaaga gttgcgccac acctgcgcgt gtcggtacaa aataggaaga gataatatcg
    11881 atgcgttcac tgggtgagcc catgatatcg aacaggcgct gcggtaacag tgaatgccgt
    11941 tttgccttgc cctccccttt cgccggatca tcacttaata aacgtgtctt cgcccagata
    12001 agcggcaatg ttccatcaac cagatgattt ataaatggac tggattccat tttgcgtaaa
    12061 taacgatgcg tcatggcatc gttatgccag gaggcgggta actcgatgcg atccgccatt
    12121 tcaccctccg ggacatccag cacctgctgt aagggtgaaa ccgatttgca ataccagtag
    12181 cgggcgaaat catcggcaac gtcctctacc acgggtccta ttgccatgac atctaaatcc
    12241 gaaaaaagtg gctcctcccc tgctccaaaa taggcatcac caatatttcg tcctcccact
    12301 agggtcacca cgccatcgac agtgaaactt ttattgtgca tacggcgatt aagacgggaa
    12361 aagtcggtga tataaccaag cggacgcagc aagcgaaacg agaaaggatt aaaaagccgg
    12421 acttcaatgc gtggatgact gtcaagcaag cgtaaaatgt cgtcaagtcc gggcgtattg
    12481 ttgtcgtcca gcaacaaacg gacgcgaacg ccacgctttg ctgcggctaa cagggcggaa
    12541 aacagtaacc gacccgacat atcgtcctgc cagatgtaat actgaacatc gagcgtatgc
    12601 tctgccattt cggccagacg ataccgggcg gcaaacgcat ccagactttt ctccagagga
    12661 aaaaggccac actgaccggg atgttgcgaa cacagtggca gcaccgcgct cgccagccgg
    12721 ggcaaatcat tcatctcctt gttgggtaag gagtctttcg tagaggtggg cgttttcttc
    12781 atcataacag acaaagtata cctgttcggg taaagcgtga cgggtaataa attctgaaac
    12841 ggtttttact gcgatttcag ccgctgccgc acgagggtaa ccataaaccc cagtactgat
    12901 tgcaggaaaa gccactgacg tatagctgtt tgccgccacc agtcgtaggc tattgagata
    12961 ggcatcctgc aaaagctggt cttcgttttg ttcaccacca cgccagactg gcccgacggt
    13021 gtgcactacg gctttagcgg gaagagcgcc tgcaagcgta ataacggcat gccccgtagg
    13081 gcaatcgccc tgctgttgcc tgacttttaa acaggcatcc agtagggccg gacccgctgc
    13141 gcgatgaatg gccccatcga cgccgccacc tcccattaat gaaggattag ccgcattcac
    13201 aatcacatca acggccagtt tggtaatatc accctgcaca acatgaatac gcgttttcat
    13261 aacgcctcct tacaccttct tgtttcttaa gtgtacagca ggctttttgg ttaaatcgca
    13321 tttttctact cagcaacgtg agttgttcag aaacttagtt atattcgatg ttcattacag
    13381 ccagcttttt ctgtttatca agatactgca cttttactga ggctatttgc gcgtttttga
    13441 cattgccgct cattaacgct ttgctggaat aggttttgcc cggaacagaa ccattctgtg
    13501 ggcagttcat ttctacgggt gatgactggg tgctgacgtc acatgccggt tccacaattt
    13561 ggccataaaa atggatcacg cccccttgcg cggtgcttcc tgcgatggca ataaaaggaa
    13621 aaaacaaact gccgagcata agagagttta aaaaaggtct gaacatttat tctatttcct
    13681 caatgattag tcatccttga gggttgtgtt atccatactt tcaggttggc tgattcgcag
    13741 cagaccattc tctccagatt catcttatgc tcgatatttc aacaaattaa gacttttctg
    13801 aagagggcgg ccattgttgt gataaatgaa gtgactgtcc atcagaaaca gtaacaacta
    13861 ttttcacccg atcgtccggg gaaatattta aactcaactt cgtcaaagca atgggttgat
    13921 tagcaggcaa tgaaagggtc ttttcttgct tcgtctgact ttgccctgaa ctgccttcgc
    13981 gcagggacaa tatttgtact ctgcacagac aagattgagt aagagtgact tcaggaataa
    14041 tggtatacac atccccttgc tgggtcgtat taaaggttat ctgactggaa agtgccgcaa
    14101 ggagtaataa cgcattcata ttcttctccc gaaaaaaaac agggcttgcg ccctgtttct
    14161 gtaatacaaa tgatgtatta gtactgatga gcggtcgcgt tgttaccaaa gccaacctga
    14221 gtcacgttga cggaggagtt agatgcagtc tggtcaactg cagcaccgtt gccaccaccg
    14281 aactgtttaa ccgtcatttc agaatttttg ccgttccact gatcaagagt agcgctgtta
    14341 ccgaagccac gttgggtcag atcgattgag ctgtcatccg agccctgacc aacatctgca
    14401 ccattaccgc cgccatgctg ggtaatagtc aagtcagagt tacgggcatc agtttgcaga
    14461 gcaagtgcag agttaccgcc accgtactgg taaatgttca gctcagaatt tgggccgcta
    14521 ttattaccgc caccaccgtg gttaccgccg ccgccgtact gaggaacaac acctgccaga
    14581 gcgctaccgg agaatacgat tgctgcaatt gctgctactt ttaaaagttt catgtaaaac
    14641 ccccatcgga ttgatttaaa agtcgaatgg aaattaacgt tgtgtcacgc gaatagccat
    14701 ttgcgactgt ctctgcacta caattgccgt tttttgagta ccatactgtg taatatttgc
    14761 tttattacca gaacctttct ggataatcat cgcagtatta ccataagcac cttgcgaaat
    14821 actggcatcg ttggcactgc ccgcctgatc aatatatgca aggttataat ctcctgtctg
    14881 gtcaatcttt gcccggttgc tactaccttc ttgcgcaaca accgccaaaa gttttgagcc
    14941 tccctgccgt aactgagcac tattattcgt cccagcttga ccaattatgg ctgcctgatt
    15001 aaatgaagac ttactcaatt catttaccgc gaagttatat tctgaattag ctaaatcata
    15061 acctgctgcg gctgcaatcc caggcgcacc cagtattgtt aacatcataa ataacaattt
    15121 gtttttcatg ttgtcaccct ggacctggtc gtacatttaa gaaattaaat catttcaact
    15181 tggttgttaa cgcaacctgt attttgttaa cgctgcgtta cgatggaaag tatgtctgcg
    15241 gaaatatttt taataactca cccgcgcgtt gtatttttct ttttttgttc ataccaaagt
    15301 attaaaaccc acgtaaatac gctgttatct acgcaaaaaa tattttgttt tcttttaaat
    15361 ctccgttttc cgctatcaaa aatcaccaga cagtcatttt tcttgcccgt cgctgattgc
    15421 tgcgatatgt cttgcgcaca agccgtgaca aacctcgtct ttttgcagta aaaaattgtc
    15481 cacggaggtg cggagaaaaa acaagaacgt tt
//
LOCUS       DBANUU010000098        15510 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000098 DBANUU010000000
VERSION     DBANUU010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.098"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            1..227
                     /locus_tag="ACIE68_003260"
                     /pseudo
     CDS             1..227
                     /locus_tag="ACIE68_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523829.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            224..571
                     /locus_tag="ACIE68_003261"
     CDS             224..571
                     /locus_tag="ACIE68_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909532.1"
                     /translation="MMTMAGKIPAFFLPVPGQSTKEVAAGSALPGHAEHGLVGVQLCG
                     RRVVCGLCRASILAELALMQATVLMGGGYLKKVRDNIMESPISSKKLTRFDTTLTGCF
                     ERDISRVGHRHFK"
     gene            640..1215
                     /locus_tag="ACIE68_003262"
     CDS             640..1215
                     /locus_tag="ACIE68_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilome CxxCx(11)CxxC protein"
                     /protein_id="HFX5909533.1"
                     /translation="MDEEKTRAEVAHIEFISYGTSRIFDRRMRSLDWKRKVVTFLGVF
                     VPLMIGCAVLSFGLEAPFLPLCITIAGVASIMQLGFSLWSLVSGWDRSYSDCMASVKE
                     NTAIYNLAGSVRKKIGKLDEAKLEILIDDLTEKFERREQEDLTLCVSDKELRYANRMS
                     CFYFKKKCHICNVVPLTLKPGKCVCDGCGKF"
     gene            1228..1440
                     /locus_tag="ACIE68_003263"
     CDS             1228..1440
                     /locus_tag="ACIE68_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909534.1"
                     /translation="MSSAKILNAYFEASEEDRKEFLRVLSHFYKDNRRVPLQEGMESF
                     AVNESIRKSQSINFAPMQGGCPLCGK"
     gene            complement(1701..2507)
                     /locus_tag="ACIE68_003264"
     CDS             complement(1701..2507)
                     /locus_tag="ACIE68_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFX5909535.1"
                     /translation="MSQPNLQCMGIDVAKLSLDIATTDTIEPFTVGNDEDGFAVITDK
                     LDAAMLAQLALVIDRHPDRSRYIRHLPDEARAVLAAMVVRRRQLNHMLVAERNRLYPS
                     HPQSRKSIDNIIDALQNELDRINEQMKQHMTAFFQEQARLIGSVKGVGDITVASLIAE
                     LPEQGKLNRREISALTGVAPLNRDSGKMRGKRTTFGGRAGVRATLNMAALVATQFNPA
                     IKLFYQRLLAAGKPKKLALVACMRKLITILNTMLRKGEEWNASFQSQVIS"
     gene            2909..3334
                     /locus_tag="ACIE68_003265"
     CDS             2909..3334
                     /locus_tag="ACIE68_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFX5909536.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGNAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            3334..3795
                     /locus_tag="ACIE68_003266"
     CDS             3334..3795
                     /locus_tag="ACIE68_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFX5909537.1"
                     /translation="MNGRGGNAAPVKETRTCTVKEIIRDVLDTDVPDSECVFAVVLTR
                     GNVRHMAQDWNLSDDELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPA
                     VLLEKVMVMAGSEIYRLHAVGSENGGDGDAFVREEREIMRVMRQALDGENG"
     gene            3795..3977
                     /locus_tag="ACIE68_003267"
     CDS             3795..3977
                     /locus_tag="ACIE68_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909538.1"
                     /translation="MNISTETREILRNYKTVINARRREMGQKPLTTAQIVDEICDFVT
                     NQQAVFLGGHYILQGS"
     gene            complement(4201..4454)
                     /locus_tag="ACIE68_003268"
                     /pseudo
     CDS             complement(4201..4454)
                     /locus_tag="ACIE68_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4971..5201
                     /locus_tag="ACIE68_003269"
     CDS             4971..5201
                     /locus_tag="ACIE68_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909539.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            5253..6614
                     /locus_tag="ACIE68_003270"
     CDS             5253..6614
                     /locus_tag="ACIE68_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFX5909540.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQTLDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLVER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTIWRAESLD
                     LNMAKLISSHDRISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HKRQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGTVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            6662..7225
                     /locus_tag="ACIE68_003271"
     CDS             6662..7225
                     /locus_tag="ACIE68_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFX5909541.1"
                     /translation="MYSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAGISNRDHILEPSAGTGAILRAIRDTAPVAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(7311..7736)
                     /locus_tag="ACIE68_003272"
     CDS             complement(7311..7736)
                     /locus_tag="ACIE68_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909542.1"
                     /translation="MAPDTTTGRNPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDR
                     GGATGTVAASQGFASGMEARQGGDSFAGSVHDSPPRQGDARKRHKQENNKTDSNQSSR
                     KKKNSSSSRNNNIISNRQMTGLKRQKPTAEFPRNERGGA"
     gene            7806..8012
                     /locus_tag="ACIE68_003273"
     CDS             7806..8012
                     /locus_tag="ACIE68_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFX5909543.1"
                     /translation="MHIPQKFLPKSRQKLLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            8038..8577
                     /locus_tag="ACIE68_003274"
     CDS             8038..8577
                     /locus_tag="ACIE68_003274"
                     /inference="COORDINATES: protein motif:HMM:NF010292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFX5909544.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGAQTQPEEGQQFSGQPQPEPQAEAGTKKGGAKTKGRGRKAAQPE
                     PQPQPPEGDDYGFSDDIPF"
     gene            8635..8868
                     /locus_tag="ACIE68_003275"
     CDS             8635..8868
                     /locus_tag="ACIE68_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFX5909545.1"
                     /translation="MNEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIGDEHGEQFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHAIRDFGTLK"
     gene            8932..10890
                     /locus_tag="ACIE68_003276"
     CDS             8932..10890
                     /locus_tag="ACIE68_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFX5909546.1"
                     /translation="MSVTESKTKTERKSSRKPSKEQETALSALLAQTAEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPDAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRTGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            10945..11379
                     /gene="psiB"
                     /locus_tag="ACIE68_003277"
     CDS             10945..11379
                     /gene="psiB"
                     /locus_tag="ACIE68_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFX5909547.1"
                     /translation="MKTELTLNVLQTMNAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVNDIIHILMAEGGQV"
     gene            11376..12162
                     /locus_tag="ACIE68_003278"
                     /pseudo
     CDS             11376..12162
                     /locus_tag="ACIE68_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            12352..12465
                     /locus_tag="ACIE68_003279"
                     /pseudo
     CDS             12352..12465
                     /locus_tag="ACIE68_003279"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            12410..12532
                     /locus_tag="ACIE68_003280"
     CDS             12410..12532
                     /locus_tag="ACIE68_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFX5909548.1"
                     /translation="MVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK"
     gene            complement(12833..13130)
                     /locus_tag="ACIE68_003281"
                     /pseudo
     CDS             complement(12833..13130)
                     /locus_tag="ACIE68_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13431..13718
                     /locus_tag="ACIE68_003282"
     CDS             13431..13718
                     /locus_tag="ACIE68_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909549.1"
                     /translation="MSTRNIHVNIASYTLLVAGKKKYTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRTGLDIRAIC"
     gene            13837..14658
                     /locus_tag="ACIE68_003283"
     CDS             13837..14658
                     /locus_tag="ACIE68_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFX5909550.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(14954..15463)
                     /locus_tag="ACIE68_003284"
     CDS             complement(14954..15463)
                     /locus_tag="ACIE68_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFX5909551.1"
                     /translation="MKKWMLAICLMFINEICLATDCFDLAGRDYKIDPDLLRAISWQE
                     SRYRVNAIGINPVTGYGSGLMQVDSQHFNELARYGIKPEHMTTDPCMNIYTGAYYLAI
                     AFKKWGVTWEAVGAYNAGFRKTERQNQRRLAYASDVYRIYTGIKSSKGIRVPTTKKSL
                     SQMNSVQNN"
BASE COUNT         3553 a         3949 c         4432 g         3576 t
ORIGIN      
        1 cacttgtatt gtcgatctga ttatcagcgt gagactacga ttccattaat gcctgccagt
       61 gagtgtgtgg aacatatctt ggccagtggc ttggtctgtg atgtgctgcg tataccgggt
      121 gaaccgcccc gccgcttgtt tgaccgctat attctgcggg agttttcttg gaggcacggg
      181 atgaaatccg cagcatggcg gatgccctgg caaaaatccg gaaatgatga cgatggcggg
      241 gaaaatcccc gcctttttcc tgccggtccc cggacagagc acaaaggaag tcgccgccgg
      301 ctccgcttta cccggccatg cggagcatgg ccttgtgggg gttcagttat gtggacgtcg
      361 agtcgtgtgc gggctgtgcc gtgcctccat cttagccgag ctggcgttga tgcaagcaac
      421 tgtcttaatg ggaggcggtt atctgaaaaa agtacgggat aatattatgg aatcgcccat
      481 ctcaagcaaa aaactgactc gcttcgatac tacgctgaca ggctgttttg agcgagatat
      541 ctctcgtgtc gggcatcgac attttaaatg attctaaata atatgagcag ggtcagtctt
      601 attaaagatt aaaacaccgg ataacaagtt ttattgatta tggatgaaga gaaaactcgt
      661 gctgaagtgg cacacataga atttatttca tatggtacgt ctcgaatttt tgataggcgt
      721 atgagatcct tagactggaa gcgtaaggtt gtcaccttcc ttggggtctt tgttccactg
      781 atgataggtt gtgcagtttt atcattcgga cttgaagctc catttctgcc gctttgcatc
      841 acaattgccg gtgtggcaag tatcatgcaa ttggggttct cgctgtggtc tttggtttcc
      901 ggatgggatc gctcatatag tgattgcatg gcttccgtta aggaaaatac agccatttat
      961 aacctagctg gtagtgttcg caagaaaata ggtaagcttg acgaagccaa attggaaatt
     1021 ttgatagacg atttgacgga aaaatttgag cgaagggagc aggaagactt aactttgtgt
     1081 gtgagtgata aagaacttcg ttatgcaaac cgtatgtcct gtttctactt caaaaaaaaa
     1141 tgccatattt gtaatgtcgt tcccctaact ttgaaaccgg gaaaatgtgt ttgtgatggt
     1201 tgtgggaaat tttaacaagg agaacatatg agtagtgcaa aaattcttaa cgcttacttc
     1261 gaggcttctg aagaagatcg aaaggagttc ctacgggttc ttagtcattt ttataaggat
     1321 aaccgaagag tacctctgca agagggcatg gagagctttg ccgtcaatga aagcatccgt
     1381 aaatcgcagt caattaactt tgcccctatg caaggtgggt gtccactctg cggcaagtaa
     1441 cgaccaaagc cgcttgacta gcggcttgat agatttcttt atattgtaac tagcatcttt
     1501 acatcctgtt aaaaaagtgg ctattgcccg ctatcttgca attctccgta tttggtacaa
     1561 aaacagccga aagctgcact atcagcagct atgagcagcg gccccactga catcgcggat
     1621 gctttgtccg tgggagggcg gatcacaaca ggcctgcggg ataagttgtt ctttctgtca
     1681 ttatgtaaaa tgaccggcag tcatgagatt acctgtgatt gaaatgaggc gttccactct
     1741 tccccttttc tgagcatggt attcagaatg gtgatgagtt tgcgcatgca ggcgaccaga
     1801 gcaagttttt tgggttttcc ggcggcaagc aaacgctggt agaacagctt tatggcagga
     1861 ttaaactgcg tagccaccag agctgccatg ttcaacgttg ctctcactcc ggctctgcca
     1921 ccaaacgtgg tccgtttccc tcgcattttc ccggagtctc tgtttagagg agcgacgcca
     1981 gttagagcac taatctcccg tcgattgagt ttcccctgtt ccggtagttc ggcaatcagc
     2041 gacgcgacgg tgatatcgcc gacgcctttc acgctgccta tcagtctggc ctgctcctgg
     2101 aagaatgctg tcatgtgttg tttcatttgc tcattgatcc ggtcgagctc gttttgaagc
     2161 gcatcaataa tgttatcgat actcttcctg ctttggggat gagaaggata gagacgatta
     2221 cgctcagcga ccagcatatg gttcaactga cgacgacgga cgaccattgc ggcaagtact
     2281 gctcgtgcct catctggcag atgccgtata taacgactgc ggtccggatg gcgatcaatg
     2341 accagggcca gttgtgctag catggcggcg tcaagtttat ctgtgataac agcgaaacca
     2401 tcctcatcgt tacccacagt gaatggctca atcgtgtcgg tggtggcgat gtccagcgat
     2461 agtttggcaa catcaatacc catgcattgc agatttggtt gactcatatg acccttcctt
     2521 acgatacgaa tttggtttca atcaactgta cggatttgtg atgagtgact cagctcatgc
     2581 gcgggacgct tgcatgcggg cttaataccc tcgggttcgc acgtgctgca tgagctttgc
     2641 cagacctaca gattcagttt atgccatctt caagatataa ggatacgctt cgccgctgcg
     2701 gtcacccggt ccctccttcc cgtctgccgt gattttccgg tcgtttaccc gctcagattt
     2761 cgcggtctca ccctccaact cccgccagcc gtgtgtgtca ggctgcggct tcccttgcgc
     2821 cctatgcatc cccgccttat cgcccggctt ttatggaggc acggcacagc ccggtgtcgc
     2881 ggaatatgtg aacaacggag gaaatcccat gcagtatgcg aaacctgtca ctctgaatgt
     2941 tgaagagtgc gaccgtctgt cttttctgcc ttacctgttt ggcaacgatt ttctgtatgc
     3001 cgaagcatat gtgtacgcgc tggcgcaaaa aatgatgccg gaatatcagg gaggcttctg
     3061 gcacttcatc cgcctgccgg acggtggcgg ttacatgatg ccggatggcg accgttttca
     3121 tctggttaac ggtgaaaact ggtttgaccg cacggtcagt gccgatgccg caggcatcat
     3181 tctgacttcc cttgtgatta accgccagtt gtggctgtac cacgacagcg gtaacgcagg
     3241 gctgacacac ctgtaccgga tgcgtgatgc gcagttgtgg agccacatcg aatttcaccc
     3301 cgaatgtaac gcgatttacg cggcgctgga ctgattaacg gacggggcgg caatgccgcc
     3361 cctgtaaagg agacgagaac atgtactgtt aaagaaatta tccgcgatgt actggataca
     3421 gacgtgccgg acagtgaatg cgtttttgcc gtggtgctga cccgtgggaa tgtgcgccat
     3481 atggctcagg actggaacct gtcagacgat gagctggaaa ccgtcatgca gcggctggac
     3541 gatgcttttg tatatggcgc atgtgacaga gttgtttccg atattgtcaa tgaactgatg
     3601 gaggaaaagc gcgtaaaccg ccttgtaaca gtccctgcgg tgctgctgga aaaagtgatg
     3661 gtgatggctg gtagtgaaat ttatcgcctc catgctgtcg ggagcgagaa cgggggcgac
     3721 ggtgacgcat ttgtcaggga agaacgtgag attatgcggg tgatgcgtca ggcgctggac
     3781 ggggagaacg ggtaatgaat atcagcacgg aaacccgcga aattctgcgt aattacaaaa
     3841 ccgtgattaa tgcgcggcgt cgtgagatgg ggcagaaacc gctcaccacc gcgcagattg
     3901 ttgatgaaat ctgcgatttt gtgacgaatc agcaggcggt tttcctcggt ggtcactata
     3961 tccttcaggg cagctgaaac aggtgataca gggcaggcgg tggaggccgc ctgtcagccc
     4021 gcgcgaaccc ccctggcagg ctgacgcctg ccggaaggga agcgggccac gctgacgcgt
     4081 cgcccgttac ccttcccgtt agtgatgtgt ccggaatgtt ttttctgatg ttatttttct
     4141 gtggttaccg tgtttgttat tgttcttctt ctttttcctc ttcctcttct ttccgttcct
     4201 ctcctgttta tggcttttcc gggcgtcccc ctggcggggc gggctgtcgt gaacggagcc
     4261 agcggagctg tctccgccct ggcgggcttc catccctgac gcgaaaccat ggcttgcagc
     4321 gaccgttccg gtcgcgccgc ctctgtcgcg ccgtggcctg cgctcccggc atcccggtaa
     4381 aaggcgccgg aatgtaagga gcaacgccgt ttcgcaaacc gcgcttcacg tcgttgcggc
     4441 gggtccctgc tcattgcctg ccaagtgcca cacacccgta tgagtccctg tcgccggggg
     4501 ctgagacatc cacaacctgc cgcactgact gtccagggta aatgaacagc ccacaaaatt
     4561 ttttacccag aacccggtaa aaaattttgc gttctgccca ggacaggtgc gtcaggccgt
     4621 ggcagtgatg ccccttgcgg cgaaggttct caaacgggtg aagttcatac cacagggaat
     4681 acagcagcag gttcccgcga aacgtcaaaa ccgcggctcc cgaaactcaa cgggtgatgt
     4741 tctttaaaaa tcaggattta tttcaggctc cggcaatacg catttatttg ttctgtcagt
     4801 catccggctg gcaggtaatg agaacgcagg aacagaagga ggaaaacacc ggaaagcgtc
     4861 agatgcagat atgaaaaaag caggtcaggg acctgcttaa tccacgccgt agaaatcgcc
     4921 gcaatcgatt ttcacggctt cactaacctg tgtcataagg agtttcattt atgacagtaa
     4981 gtattgtttc accttccgca gcggctgtaa agccccgccg tcatccccgt ttcagacgcg
     5041 aagacatccc cgcgccggaa attgaccccg tgctgaaagc attcgggcga cacatagccc
     5101 ggagttttca ccggggacgt ggtgtacaca tccccgccat gaaaaacacg gctttcgggc
     5161 aggtgctccg cacacttgaa ctgaaacgcg cttttaactg atgaggtgag ccgccccgcg
     5221 aggggcggca acagacggaa ggagaacatt ttatgacatt agcagcaatc accatgaccg
     5281 ccccggaagc cgccagccct gtgcagatgt accgcgcgac ctactcaccg gatgacaaca
     5341 aactgcgcct gtatgctgcg tcacgtcttg acccggagac gtataaaaaa gtgcatgatg
     5401 ccggttttcg ctgggcacca aaacaggcgc tgtttgtggc tccagcctgg acaccgggcc
     5461 gggaagacgt gctcctctca cttgccggag agattgagga tgaagacagc acgctcgctg
     5521 aacgtcagga agcacgggcg gagcggttta ccggatacag cggaaagcgg gccagtgaat
     5581 ccgcacagac acttgatgaa gtggaaagac tggccgcgat gatcccgccc ggtcagccca
     5641 ttcttgtggg gcatcacagc gaacgccgcg cccgtcgtga tgcgcagcgt attgaaaacg
     5701 gcatgaaacg tgccgtgatg ctcgttgaac gtgcggaata ctgggaagag cgggcgcggt
     5761 cggcactgct tcacgcgaag tataaagaac gtccggacgt tcgctggcgt cgtatcaaaa
     5821 aaatcgaagc tgatttgcgc aaggctgaaa agaccatcgc gcagtcgcag aaatatctga
     5881 cgatatggcg ggctgaatcg ctggatctga atatggcaaa actcatcagt agtcatgacc
     5941 gtatcagtgc ctgtttcccg ctggatacgt atccgcgccc ggcagaaaaa agccagtatg
     6001 aaggaagtcg atcgttatgg tcggccctgg atgatgacat catcaccacg gagcaggccc
     6061 gcgaaattgc gatccgctgt cataaacggc agattcagca tcagcaacgc tgggttaacc
     6121 actatcagaa ccgcctgatc tatgagcgtg ccatgctgga cgaaagcggc ggcgtggtta
     6181 cccggacaca ggattttgag ccgggcggac aggttttcag ccggggcgag tggctgacca
     6241 tcatccgcgt gaacaaaagc aacgggacgg tgagttcagt cacaacgccg aattacagtt
     6301 ttctcgggta cagtggcacg atgaaagtga cgcccgatcg cattacggac tacaaagcac
     6361 catcggcaga agaggctgcc gtcgccagtc aggccgctaa gcgtccgcct gttgtcaact
     6421 atccgggaga aggtttccgg gaaatgacaa aggcacagtg ggctgccctg ccccgggact
     6481 gtaaggccgt gcgcagtgtg gcagaggcag aagaccacgg ggcatatcgt taccgccgca
     6541 caatggacaa taatttccgc ctggtgaatg tgtatatcac cgacatgaag attacggaaa
     6601 tcccacagaa ataaggtata tcctcccggg aaaatccccg ggggatgacg gagataatga
     6661 catgtacagt cagttaagag aacgtatccg gctgatgcgc gcaaggctgg ataacgccgc
     6721 gccggttgct gaaatccggg ctgaatctca gctttttgtg actcctgccc cggtctgtga
     6781 tcgcctggtg acgctggcgg ggatcagcaa ccgtgatcac attctggaac cctctgccgg
     6841 caccggggcc atactgcggg cgattaggga taccgcgccg gtagccatgt gtgatgcggt
     6901 ggaaatcaac agcgggctgg tccggtatct gcgggaaaat tttaacggtg tcagggttca
     6961 gtgtggcgac ttcatggaat ggcagccggt gcagtattac agccgggtta tcatgaatcc
     7021 gccgttcagc cacgggcagg atatccggca tatcctgcgg gccttttccc tgttgcgtcc
     7081 gggtggcgtg ctggtcgccg tctgtctcaa cgggccacgc cagcaggaga agctgttacc
     7141 gttttctgac gtccgcgagg agctgccgcg cggcacattt gcttataccg atgtcccgac
     7201 gatgattatt cgtctgcgtg cctgaagact ccgggcaggt gggaggataa atgctcgtct
     7261 gcccgcgccg ggaaaagacc gcgcgccggt ggcgctgaaa ggggccgcgc tcacgcgccg
     7321 cccctttcat tacgcggaaa ttctgctgtg ggcttctgtc ttttcagacc tgtcatttgt
     7381 ctgttactga tgatgttgtt gttcctgcta ctgctactgt tcttcttttt tctgctgctc
     7441 tgattactgt ctgttttgtt gttctcctgc ttatgccttt tccgggcgtc cccctggcgg
     7501 ggcgggctgt cgtgaacgga gccagcgaag ctgtctccgc cctggcgggc ttccatccct
     7561 gacgcgaaac cctggcttgc agcgaccgtt ccggtcgcgc cgcctctgtc gcgccgtggc
     7621 ctgcgctccc ggcatcccgg caaaaggccg ccgggatgta aggggcaacg ccgtttcgca
     7681 aaccgcgctt cacgtcgttg cggcgggttc ctgcccgttg ttgtgtccgg tgccattcac
     7741 ctgtttaagc ccctgtcgcc gggggctgat acagccacaa ccgtctccgc cagtccaggg
     7801 gtgaaatgca catcccgcaa aagtttttgc ccaaaagcag gcaaaaactt ttgcggtctg
     7861 cccccggaca gtcgtttccg gccgtggcag tgatgcccct tgcggcgaag gttctcaaaa
     7921 aggtgatggt gaataccgga gggaacaaaa ggcaggtccc cgcaaaacgt caaaaccgcg
     7981 gctcccgaaa ctcaaaagat gatgggggct gaaaacatca acagaaggag gcacatcatg
     8041 gcagttcgtg gcattaacaa ggtcatcctc gtaggacgcc tgggaaaaga tccggaagtc
     8101 cgttacatcc ccaacggggg cgcagtggca aacctgcagg tggccacgtc agaaagctgg
     8161 cgtgacaaac agacggggga gatgcgggag cagacggaat ggcaccgcgt ggtgctgttc
     8221 ggcaagctcg cggaagtggc aggtgaatat ctgcgcaagg gcgcgcaggt ctacatcgaa
     8281 ggtcagcttc gcacccgtag ctgggaggat aacggtatca cccgttatgt caccgaaatt
     8341 cttgttaaga ccacgggcac catgcagatg ctgggacgtg ccgcaggtgc tcagactcag
     8401 ccggaagagg ggcaacagtt cagcggtcag cctcagccgg aaccacaggc ggaggccggt
     8461 acgaaaaaag gaggcgcaaa aacgaaaggc cgtggacgta aggccgcgca gccggagcct
     8521 cagccgcaac cgccggaggg tgacgattac gggttttcag acgatatccc gttctgatcg
     8581 ggctgactgt gataaccgcc ccgccctgtg cggggcatca ccggagagat gaggatgaac
     8641 gaatatttca gaatacttca gggactgcct gacggtcctt ttacccgcaa acatgccgaa
     8701 gccgttgccg cacagtaccg gaacgtcttt atcggggatg agcacggaga acagtttcgc
     8761 ctggttgtcc gtaataacgg aacaatggtc tggcgtacct ggaattttga ggacggtgcc
     8821 gggtactgga tgaaccatgc catccgtgat ttcggcactc ttaagtaaga gaggtgccgg
     8881 acgggaacgc gtccggcaga catacaacca tcccggtcaa aggagaaata tatgtcagtt
     8941 acagagtcta agacaaaaac ggagcgtaaa tccagccgta aaccgtcaaa agagcaggag
     9001 actgccctgt ctgcactgct ggcgcagacg gcggaagtga gcgtgccgct ggcctcactg
     9061 attaaatcac cgctgaatgt gcgcacggtg ccgtattctg cggagtccgt cagcgaactg
     9121 gcggactcca ttaaaggtgt cggcctgtta cagaatctgg ttgttcatgc cctgccaggt
     9181 gaccgttacg gtgtcgccgc aggcggtcgc cgactggcag cactcaacat gctggcagag
     9241 cgcaacatcc ttccggctga ctggcctgta cgcgtgaagg tcattccgca ggagctggcg
     9301 actgccgcat cgatgaccga gaacggtcag cgtcgggata tgcaccctgc cgaacagatt
     9361 gccggattcc gcgcaatggc gcaggaaggc aaaacacctg cacaaatcgg tgatttgctg
     9421 ggctattcac cccgccacgt tcagcgaatg ctgaaactgg ctgaccttgc tccggttatc
     9481 ctcgatgcgc tggcagaaga ccgcatcacc accgagcact gtcaggcgct ggcgctggag
     9541 aacgacaccg cgcgtcaggt acaggtgttt gaagccgcct gtcagtcggg atggggcgga
     9601 aaaccggatg tgcgggttat ccgcaatctg attacggaaa gtgaagtggc ggtggcgggg
     9661 aacagtaaat tccgcttcgt gggggctgat gctttctcac ctgacgaact gcgtactgat
     9721 ttgttcagcg atgacgggga cggttatgtc gaccgcgtgg cgctcgatgc tgccctgctg
     9781 gaaaaactcc aggctgtcgc tgaacacctt cgggaagccg aaggctggga atggtgcgcc
     9841 ggacgcatgg agcctgtcgg tgagtgccgt gaggatgccg gaacataccg ctgtctgccg
     9901 gagccggacg cggtgctgac ggaggcggaa gaagaacgcc tgaacgaact gatgacgcgt
     9961 tacgacgcgc tggaaaacca gtgtgaggaa tccgacctgc tggaagcaga aatgaagctg
    10021 atgcgctgca tggcgaaggt cagagcgtgg acgccggaga tgcgtaccgg aagcggtgtg
    10081 gtggtgtcct ggcgttacgg taatgtgtgt gtccagcgtg gtgtgcagtt gcgcagcgag
    10141 gatgacgcgg ctgacgacgc tgaccgcacg gaacaggtgc aggagaaagc atcagtggag
    10201 gaaatcagtc tgccactgct gacgaaaatg tcttccgaac gcacgctggc agtccaggcg
    10261 gcactcatgc agcaaccgga caaatccctg gcactgctgg catggacgct ctgcctgaat
    10321 gtgtttggca gcggagcgta cagtaaacca gcacaaatca gcctggaatg taaacattat
    10381 tcgctgacca gcgatgcgcc gtcagggaag gaaggtgccg cgttcatggc gatgatggca
    10441 gaaaaagccc gtcttgcggc cctgttaccg gagggatggt cacgggacat gacgacgttc
    10501 ctgtcactca gtcaggaggt gctgttatcc ctgctcagtt tctgcaccgc gtgcagcatc
    10561 cacggcgttc agacccgcga gtgtggtcac acgtcacgca gcccgctgga cacgctggag
    10621 agcgccatcg gttttcacat gcgtgactgg tggcagccga caaaagcaaa cttcttcgga
    10681 cacctgaaaa agccgcagat tatcgcagct ctgaatgatg ccggactgtc cggtgccgca
    10741 cgggatgcag agaagatgaa gaaaggtgat gcggctgaac atgcagagca ccatatgaaa
    10801 gacaaccgct gggtgcctgg ctggatgtgt gcaccacatc cacagacgga taccactgaa
    10861 cgcaccgata acctggctga tgccgcctga tgaacaacca caccgccccg ccggagacgg
    10921 ggcagcagca agggagatac cgtgatgaaa actgaactga ccctgaatgt attacagacc
    10981 atgaacgcac aggaatatga agatatcagg gctgccggaa gtgatgaacg ccgtgagctg
    11041 acacacgccg tgatgcggga actggatgcg ccggacaact ggacgatgaa tggcgagtac
    11101 ggcagcgagt tcggcggctt tttccccgtc caggtccgtt tcacgcctgc ccacgaacgt
    11161 tttcacctgg cgttatgttc accgggtgat gtctcccagg tctgggtgct ggttctggtg
    11221 aatgccggtg gtgagccgtt tgcggtggtt caggtacagc ggcggtttgc accggaagca
    11281 gtcagccatt cactggcact ggcggcgtca ctggatgcgc aggggtacag cgttaacgac
    11341 atcatccata tcctgatggc ggaaggaggt caggtatgaa cgcacgttca cgggcactgg
    11401 ttcccctcag cacagagcaa caggccgcat ggcgggcggt ggcggagacg gaaaaacgcc
    11461 gccatcaggg aaacacgctg gcggaatatc cgtatgcggg agcatttttc cgctgtctga
    11521 acggaagccg ccggatttcg ctgtctgacc tgcgattttt catgccgtcc ctgaccgcag
    11581 aagaactgca cggcaaccgt cttcagtggc tgtgtgccat cgatgtgctg attgaaacac
    11641 agggcgaggt ctgcctcctt ccccttcccg gcgatgcagc agaacgcttg ttcccgtcgg
    11701 tgcggtttcg tgtcagggaa cgcagtcgcc ataaatccgc gctggtcatg cagaagtaca
    11761 gccgccagca agcgagggag gcagagcaga aagcccgtgc gtaccaggtg ctggttgctc
    11821 aggctgaaat cgaactggca tttcattcac cggaaacagt cggaagctgg catgcacgct
    11881 ggagtgaccg ggtggcagag catgacctgg aacccctgtt ctggcagtgg ggcgagcgtt
    11941 ttccgtcact ggccggaatg gaacgctggc agtggcagga tatgccattc tggcaggtta
    12001 tcgcggaagc cagtctggca gccagagagg ccggtcatgc tgtccgtgag atggagcggt
    12061 ggatggtgcc gaacaaactc cgggaggcag cgtgatgcgg caataatcac acggattacc
    12121 cgtgaactgg ttgaacgacc agattatttt cagggaaagt gagtgtggtc agcgtgcagg
    12181 gatatgagct atgatgtgcc cggcgcttga ggctttctgc ctcatgacgt gaaggtggtt
    12241 tgttaccgtg ttgtgtggca gaaggcagaa agccccgtag ttaatttttc attaacccac
    12301 gaggccccct gtatgtctga tcaacaacag tatggcctct taccgtgctt aatgcaagga
    12361 ggagtaaacc atgaaactac cgggaaacgc ccttatctgg tgcgtattaa tcgtgtgctg
    12421 cacgttgtta atattcacac tcctgacccg gaatcgtctg tgcgaagtcc ggctgaagga
    12481 cggatacagg gaggttacgg caagtttggc ttacgaatcc agcggtaagt agcaacctgg
    12541 aggcgggcgc aggcccgcct tttcagggct gatgctggtc tgactgcact gaagcgcctt
    12601 tataaagggg ctgctggttc gccggtggcc cctttctcct tgctggcatt gtgcgggcat
    12661 gaacaaccag actgccagga aggccgtttc cctccgggaa cggggatgaa cgtgcgcctg
    12721 cggcgcccgt tcttttcccc cgccttctct gtttatgacc tgtcagaaat cacatcacct
    12781 tccctttgat tattctcttt ttcctcctgt tctgactctg attactgctg ccctgattat
    12841 tgtctgtttt gttgttctcc tgcttatgcc ttttccgggc gtccccctgg cggggcgggc
    12901 tgtcgtgaac ggagccagcg gagctgtctc cgccctggcg ggcttccatc cctgacgcga
    12961 aaccccgggc ttgcagcgac cgttccggtc gcgccgcctc tgtcgcgccg tggcctgcgc
    13021 tcccggcatc ccggcaaaag gccgccggga tgtaaggggc aacgccgttt cgcaaaccgc
    13081 gcttcacgtc gttgcggcgg gttcctgccc gttgttgtgt ccggtgccat tcacctgttt
    13141 aagcccctgt cgccgggagc tgatacagcc acaacctgcc gcactgactg tccagggtaa
    13201 atgaacagcc cacaaaattt tttacccaaa acccggtaaa aaattttgcg ttctgcccag
    13261 gacaggtgcg tcaggccgtg gcagtgatgc cccttgcggc gaaggttctc aaacaggtga
    13321 tggtgaatac cggagggaac aaacggcagg tccccgcaaa acgtcaaaac cgcggctccc
    13381 gaaactcaac agatgatggg ggctgaaaac cagagagagg agaaagcagg atgagtaccc
    13441 gaaatatcca cgttaacatc gcgtcgtaca cgcttcttgt tgccgggaag aaaaaataca
    13501 ccggggaaga gtgggacgtc ctggaattca gcagcctgac tgagctgaaa aaatatcgca
    13561 aaagccaccc ggaaaagatg gccttcagct atagctatgc gctcagccgg ggtgtggata
    13621 cgcagttccg ccatatcaac attgcggaag ccgatcactt caaacagttc ctgcgtcaga
    13681 ttaagcgtac cggcctcgat atccgggcta tctgctgacc ggatgtgatg aatatccctc
    13741 ctgattgttg caatcttgtt aatggagatc gggagggatg atattaagcg gaaaaatttt
    13801 ttattaaatt tattctttca taaaggagta gctgttatgc gattagcttc ccgttttggt
    13861 cggtataatt ccatccgccg tgaacgtcct ttaacggatg atgaattaat gcagtttgtg
    13921 ccttcggtgt tttccggtga taaacatgag tcccggagtg aacgttatac gtatattcca
    13981 acaatcaata tcatcaataa gttacgtgat gaaggtttcc agccattctt tgcctgtcag
    14041 agtcgggttc gtgatttggg acgtcgcgaa tacagtaaac atatgttacg tcttcgcagg
    14101 gaagggcata ttaacgggca ggaagttcct gaaattatcc tgcttaattc acatgatggt
    14161 tcatccagtt atcagatgat ccccggaatt tttcgttttg tctgcacaaa tggcctggtg
    14221 tgcggaaata attttggcga aatccgcgtt ccacataaag gtgatattgt cgggcaggtt
    14281 atcgagggag cgtatgaagt gctcggtgtc tttgataagg tcactgataa tatggaggcg
    14341 atgaaagaaa ttcatcttaa cagtgacgag caacatttat ttggcagagc tgcactgatg
    14401 gtcaggtatg aagatgaaaa taaaacgccg gtgacgcctg aacagataat tactccccgt
    14461 cgtcgggagg ataaacagaa cgatctctgg acaacctggc agcgggttca ggagaatatg
    14521 ataaaaggtg gattatcggg gcgaagtgcc tccgggaaaa ataccaggac aagagccatt
    14581 acaggtattg atggtgatat aagaatcaac aaagcattat ggatgattgc cgaacagttc
    14641 agggagtgga agtcgtgata atataggtaa agccccgaaa aattttcggg gctttactta
    14701 tattgtcatc cttcggcatt gctatgtcgt ctgatatgcc tcaatttcat caactggctt
    14761 ttcggtagtc tgtaaatatt gtgcccgcga aaaatggtct ggcaggaggc tacgcagtta
    14821 ccggatatat ataatatggg ttcccgcttt gcggggcttc ggccagtcag cattatcact
    14881 catatgatat ttttgtgtgt ggccttccat gccgctgtcg cggcataacc ggtatttgac
    14941 aatgtataca atattaattg ttctgcacac tgttcatttg ggaaagtgat ttcttcgtgg
    15001 tcggaacccg gataccttta ctgcttttta tcccggtata aatccggtaa acatctgatg
    15061 cgtaggcaag acgcctctgg ttctggcgtt cggttttcct gaatccggca ttgtatgcac
    15121 caacggcctc ccaggtgacg ccccattttt taaatgctat tgccagataa taagcaccgg
    15181 tataaatgtt catgcaggga tctgttgtca tatgttccgg cttaattccg tagcgggcca
    15241 gttcgttaaa atgctgggaa tctacctgca tcagtccgct gccatatccc gttaccggat
    15301 taataccgat ggcattaacc cggtaacggg attcttgcca tgatattgct ctcagtaaat
    15361 caggatctat tttgtaatcc ctgcctgcaa gatcaaagca atcagtggcc agacagatct
    15421 catttataaa catcaggcag atggctaaca tccatttttt catttttcca cctctggtga
    15481 ctttatccgt aaataattta actcactcca
//
LOCUS       DBANUU010000099        15483 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000099 DBANUU010000000
VERSION     DBANUU010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.099"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            420..2051
                     /gene="oppA"
                     /locus_tag="ACIE68_003285"
     CDS             420..2051
                     /gene="oppA"
                     /locus_tag="ACIE68_003285"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFX5909552.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2137..3057
                     /gene="oppB"
                     /locus_tag="ACIE68_003286"
     CDS             2137..3057
                     /gene="oppB"
                     /locus_tag="ACIE68_003286"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFX5909553.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3072..3980
                     /gene="oppC"
                     /locus_tag="ACIE68_003287"
     CDS             3072..3980
                     /gene="oppC"
                     /locus_tag="ACIE68_003287"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFX5909554.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3992..5005
                     /gene="oppD"
                     /locus_tag="ACIE68_003288"
     CDS             3992..5005
                     /gene="oppD"
                     /locus_tag="ACIE68_003288"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFX5909555.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5002..6006
                     /gene="oppF"
                     /locus_tag="ACIE68_003289"
     CDS             5002..6006
                     /gene="oppF"
                     /locus_tag="ACIE68_003289"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFX5909556.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6059..6388)
                     /gene="yciU"
                     /locus_tag="ACIE68_003290"
     CDS             complement(6059..6388)
                     /gene="yciU"
                     /locus_tag="ACIE68_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFX5909557.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6423..7883)
                     /gene="cls"
                     /locus_tag="ACIE68_003291"
     CDS             complement(6423..7883)
                     /gene="cls"
                     /locus_tag="ACIE68_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFX5909558.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLILDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8026..8199
                     /gene="yciY"
                     /locus_tag="ACIE68_003292"
     CDS             8026..8199
                     /gene="yciY"
                     /locus_tag="ACIE68_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFX5909559.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8254..9507)
                     /gene="kch"
                     /locus_tag="ACIE68_003293"
     CDS             complement(8254..9507)
                     /gene="kch"
                     /locus_tag="ACIE68_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFX5909560.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(9807..10103)
                     /gene="yciI"
                     /locus_tag="ACIE68_003294"
     CDS             complement(9807..10103)
                     /gene="yciI"
                     /locus_tag="ACIE68_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFX5909561.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10327..11046
                     /gene="tonB"
                     /locus_tag="ACIE68_003295"
     CDS             10327..11046
                     /gene="tonB"
                     /locus_tag="ACIE68_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFX5909562.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPAVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(11086..11484)
                     /gene="yciA"
                     /locus_tag="ACIE68_003296"
     CDS             complement(11086..11484)
                     /gene="yciA"
                     /locus_tag="ACIE68_003296"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFX5909563.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11589..12128)
                     /gene="yciB"
                     /locus_tag="ACIE68_003297"
     CDS             complement(11589..12128)
                     /gene="yciB"
                     /locus_tag="ACIE68_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFX5909564.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12158..12901)
                     /gene="yciC"
                     /locus_tag="ACIE68_003298"
     CDS             complement(12158..12901)
                     /gene="yciC"
                     /locus_tag="ACIE68_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFX5909565.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(12929..13018)
                     /locus_tag="ACIE68_003299"
                     /pseudo
     CDS             complement(12929..13018)
                     /locus_tag="ACIE68_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            13258..13896
                     /gene="ompW"
                     /locus_tag="ACIE68_003300"
     CDS             13258..13896
                     /gene="ompW"
                     /locus_tag="ACIE68_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFX5909566.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(13956..14462)
                     /gene="yciE"
                     /locus_tag="ACIE68_003301"
     CDS             complement(13956..14462)
                     /gene="yciE"
                     /locus_tag="ACIE68_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFX5909567.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(14508..15008)
                     /gene="yciF"
                     /locus_tag="ACIE68_003302"
     CDS             complement(14508..15008)
                     /gene="yciF"
                     /locus_tag="ACIE68_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFX5909568.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(15094..15273)
                     /gene="yciG"
                     /locus_tag="ACIE68_003303"
     CDS             complement(15094..15273)
                     /gene="yciG"
                     /locus_tag="ACIE68_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFX5909569.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
BASE COUNT         4124 a         3609 c         3737 g         4013 t
ORIGIN      
        1 tgcttagcca atctctattt gattgaattg aaagatgttt gttaaggcat ggatgcaagc
       61 tatagattct gatacggtca ataaaagaga attgcttaac aattttgcaa aatgtattgg
      121 cgagtaagaa ccgcatttgg tactttccgg gcaaccgcca gacgattctt tattggtaat
      181 gagaataatt aacaattaaa gagcgtcgcg aaagaataat gtgtctcgac aggggagaca
      241 cagtacgaat cgacataagg tgatcgtctg aatcaccaga ataaataaag tcggtgatag
      301 taatacgtaa cgataaagta acctgacagc agaaagtctc cgagcctgtg cagggtccca
      361 atccgggatt acacatgctg gttaatacca gtaattataa tgagggagtc caaaaaacaa
      421 tgaccaacat caccaagaga agtttagtag cagctggcgt tctggctgcg ctaatggcag
      481 ggaatgtcgc gctggcagct gatgtacccg caggcgtcac actggcggaa aaacaaacac
      541 tggtacgtaa caatggttca gaagttcagt cattagatcc gcacaaaatt gaaggtgttc
      601 cggagtctaa tatcagccga gacctgtttg aaggcttact ggtcagcgat cttgacggtc
      661 atccagcacc tggcgtcgct gaatcctggg ataataaaga cgcgaaagtc tggaccttcc
      721 atttgcgtaa agatgcgaaa tggtctgatg gcacgccagt cacagcacaa gactttgtgt
      781 atagctggca acgttctgtt gatccgaaca ctgcttctcc gtatgccagt tatctgcaat
      841 atgggcatat cgccggtatt gatgaaattc ttgaagggaa aaaaccgatt accgatctcg
      901 gcgtgaaagc tattgatgat cacacattag aagtcacctt aagtgaaccc gttccgtact
      961 tctataaatt acttgttcac ccatcaactt caccggtgcc aaaagccgct atcgagaaat
     1021 tcggcgaaaa atggacccag cctggtaata tcgtcaccaa cggtgcctat accttaaaag
     1081 attgggtcgt aaacgaacga atcgttcttg aacgcagccc gacctactgg aacaacgcga
     1141 aaaccgttat taaccaggta acctatttgc ctattgcttc tgaagttacc gatgtcaacc
     1201 gctaccgtag tggtgaaatc gacatgactt ataacaacat gccgatcgaa ttgttccaga
     1261 agctgaaaaa agagatcccg gacgaagttc acgttgatcc atacctgtgc acttactatt
     1321 acgagattaa caaccagaaa ccgccattca acgatgtgcg tgtgcgtacc gcactgaaac
     1381 taggtatgga ccgcgatatc attgttaata aagtgaaagc gcagggcgac atgcccgcct
     1441 atggttacac tccaccgtat actgatggcg caaaattgac tcagccggaa tggtttggct
     1501 ggagccagga aaaacgtaac gaagaagcga aaaaactgct ggctgaagcg ggttataccg
     1561 cagacaaacc gttgaccatc aacctgttgt ataacacctc cgatctgcat aaaaagctgg
     1621 cgattgctgc ctcttcattg tggaagaaaa acattggtgt aaacgtcaaa ctggttaacc
     1681 aggagtggaa aacgttcctc gacacccgtc accagggtac ttttgatgtg gcccgtgcag
     1741 gctggtgtgc tgactacaac gaaccaactt ccttcctgaa caccatgctt tcgaacagct
     1801 cgatgaatac cgcgcattat aagagcccgg cctttgacag cattatggcg gaaacgctga
     1861 aagtgactga cgaggcgcag cgcacagctc tgtacactaa agcagaacaa cagctggata
     1921 aggattcggc cattgttcct gtttattact acgtgaatgc gcgtctggtg aaaccgtggg
     1981 ttggtggcta taccggcaaa gatccgctgg ataataccta tacccggaat atgtacattg
     2041 tgaagcacta atggcaatac gtggggcagg agtgtcctgc tccacggtgt ctgattttta
     2101 tcgcattaca gaaggcacag gccagaaggt agggcaatgt taaaatttat tctacgtcgc
     2161 tgtctggaag cgattccgac gctatttatt cttattacta tttcgttctt tatgatgcgc
     2221 ctcgcgccgg gaagcccttt taccggcgaa cgtactttac cgcctgaagt gatggccaat
     2281 atcgaagcga aatatcatct taatgatccg atcatgacac agtatttcag ctacctgaaa
     2341 caactggcgc acggtgattt cggtccatcg tttaaatata aagattattc ggtcaatgac
     2401 ctggtggcat ccagttttcc cgtttctgcc aaactgggag ccgcagcatt tttccttgcg
     2461 gtaatactgg gtgttagtgc tggcgttatt gccgcattaa aacaaaacac caaatgggac
     2521 tataccgtga tggggctggc aatgaccggg gttgttatcc ccagttttgt ggttgcgcca
     2581 ttattagtca tgatatttgc gatcattttg cattggctgc cgggcggtgg ctggaatggt
     2641 ggggcgctta aattcatgat attgccgatg gtggcgttgt cactcgccta tatcgccagt
     2701 attgcgcgta ttacccgtgg ctctatgatt gaagtattac actccaattt tattcgtact
     2761 gcccgggcga aagggttacc tatgcggcgg atcattttac gccacgcatt aaaacctgct
     2821 ctgttacccg tgctctccta tatgggcccc gcatttgtcg gcattattac cggttctatg
     2881 gttatcgaaa ccatttatgg tttgccgggg attgggcaac tgttcgttaa tggcgcattg
     2941 aaccgtgact attccttagt gttaagcctg accatcctgg ttggtgcttt aaccattttg
     3001 tttaatgcca ttgtcgatgt gctatatgcg gttatcgacc cgaaaatccg ttactgatac
     3061 tggagctcgc gatgatgtta agtaagaaaa acagcgagac gctggaaaat ttcagtgaaa
     3121 agctggaggt cgaagggcgc agcttgtggc aggacgcacg tcgacgtttt atgcataacc
     3181 gtgcggcggt tgccagtctg atagtgctgg tgctgatcgc gttatttgta atcctggcac
     3241 cgatgctttc gcagtttgcc tatgacgata ctgactgggc gatgatgtcc agcgccccgg
     3301 atatggagtc cggtcactac tttggtactg actcatccgg tcgcgacctg cttgtgcgcg
     3361 ttgcgattgg cgggcgtatc tcactcatgg tcggtgttgc tgcggcactg gtggcagtgg
     3421 tcgtcgggac actttatggt tcgctttccg gttatctggg cggtaaagtg gattcggtaa
     3481 tgatgcgtct gctggaaatc ctcaactcct tcccattcat gttcttcgtc attttgctgg
     3541 tgaccttttt cggtcaaaac atcctgctga ttttcgtggc gataggcatg gtttcctggc
     3601 tggatatggc tcgtattgtg cgtgggcaaa ccctgagtct gaagcgcaaa gagtttattg
     3661 aggcggcaca agttggcggt gtatcgacat cgggcattgt tattcgccac attgtgccga
     3721 atgtactcgg tgtggtggtg gtctacgcat cacttctggt gcccagcatg atcctctttg
     3781 aatctttcct tagcttcctg gggttgggta cgcaagagcc gttaagcagc tggggtgcat
     3841 tgctgagtga tggcgcgaac tcgatggaag tctctccatg gttattgttg ttcccagcgg
     3901 gattcctcgt ggtgacgctg ttttgtttca actttatcgg cgatggcttg cgtgatgccc
     3961 tcgacccgaa agatcgttaa ggagtgcagc catgagcgta attgaaactg caactgtgcc
     4021 gctcgcacaa caacaggctg acgcactgct gaacgtgaaa gatttgcgtg tcacctttag
     4081 taccccggac ggcgacgtca cggcggtcaa tgatttgaat ttttccctac gtgccggaga
     4141 aacgctgggt attgtaggtg agtctggttc gggtaaatcg caaactgcat ttgcgttgat
     4201 gggcctgttg gctgccaacg gacgtattgg cggatcggca accttcaatg ggcgtgaaat
     4261 cctcaatttg ccagagcatg aactcaataa actgcgcgct gaacaaatct caatgatttt
     4321 tcaggaccca atgacttcgt tgaatcccta tatgcgcgtc ggtgagcagt taatggaagt
     4381 gctgatgctg cataagaaca tgagcaaagc tgaggcgttt gaagagtcgg tgcggatgct
     4441 cgatgcggta aaaatgccgg aagcgcgtaa acgcatgaaa atgtacccgc acgaattttc
     4501 tggcggcatg cgtcagcgag tcatgattgc gatggcattg ctatgtcgac ctaagctact
     4561 gattgcggat gaacccacta ctgcgctgga cgtcaccgta caggcgcaga tcatgacgct
     4621 attaaatgaa ctgaagcggg aatttaatac cgccatcatt atgatcaccc acgatcttgg
     4681 cgtggtggcg gggatctgtg acaaagtgct ggtaatgtac gccgggcgca cgatggaata
     4741 tggcaatgcg cgcgatgtct tttatcaacc cgttcatcct tattctatcg gtttgctcaa
     4801 cgcggtgccg cgtcttgatg cggaaggtga aacaatgttg accatcccgg gtaatccgcc
     4861 aaacttgctg cgattaccga aaggttgccc gttccagcca cgttgtccac atgcgatgga
     4921 aatttgtagt agcgcaccgc cgctggaaga gtttacgcct ggccgtctgc gtgcttgctt
     4981 taaaccggtg gaggaactgt tatgaatgct gtaactgaag gaagaaaagt cctccttgaa
     5041 atcgccgatc ttaaagtgca ctttgaaatc aaagatggca aacagtggtt ctggcaaccg
     5101 ccgaaaacgc tcaaagccgt cgatggtgtc actcttcgcc tgtatgaagg ggaaacatta
     5161 ggtgtggtag gggaatcggg atgcggtaag tccacctttg ctcgcgccat catcggtttg
     5221 gtcaaggcga ccgacggtca tgttgcctgg ttaggtaaag agttgctggg catgaagccc
     5281 gatgaatggc gtgccgttcg cagtgatatt cagatgattt tccaggatcc gttggcatcg
     5341 ctaaacccgc gtatgaccat cggcgagatc atcgctgaac cactgcgtac ttatcatccg
     5401 aaaatgtcac gccaggaagt tcgcgagcgc gtgaaggcga tgatgctgaa agtcgggtta
     5461 ttgcctaacc tgattaaccg ctatccgcat gagttctccg gtgggcagtg ccagcgtatc
     5521 gggattgctc gtgctcttat tcttgaaccg aagctgatta tctgcgatga gccggtttcg
     5581 gcgctggacg tgtcaattca ggcgcaggtg gtcaacctgc tccagcagct gcaacgtgag
     5641 atgggattgt cattaatttt tatcgctcat gacctggccg tggtaaaaca catttccgat
     5701 cgtgtgttgg tgatgtatct cggccatgcg gtagaactgg ggacctatga tgaggtctac
     5761 cacaatccac tacatcctta caccagggca ttgatgtcgg cagtccccat acctgatccg
     5821 gatctggaga agaacaaaac catccagtta ctggaagggg aattaccgtc gccgatcaac
     5881 ccgccttccg gttgtgtttt ccgtacccgt tgcccgattg ccggtccgga gtgcgccaaa
     5941 acacgtcctg ttctggaggg gagtttcaga cacgccgttt cttgcctgaa agtcgatccg
     6001 ctttaaacac aataagggct gacagttgtc agcccttttt cacgctaaaa gcgattattt
     6061 attcccgcca gatgatatgg caaagtttgt gatctttttc gcggcataac agaatgcggg
     6121 cgaaaacatc gttgatttca ccatcttcac tgtccgccag accaatcacc acttcggcaa
     6181 aaaagtctgg gttcaaatcg aaatcaacat gttcctgcca gtcttccgcc ggatcgaata
     6241 actccgcgcc gccgcgctct tcaaactgaa gattgaacag cagaacatca gctggatcga
     6301 gattgtcggc agccagttcg agaaaaatat cgtaggcctg ctcaagcgtt tcatcttcag
     6361 tcaggcgatt gtttagatcc atatccatag tcactacctg tttaacctct gttggcgacg
     6421 ttttacagca acggactgaa gaagtaaaac agtcgctcgg cgacacgttg ccataatgga
     6481 cgttttagcc ataaacgggc atcgagcaga cgtgaacgcg aaatataatc gtcctgaacg
     6541 gcggcaaggt ctgcaccaaa acctttatcg tcgattgcca gggtaatctc gaaattcagc
     6601 cacagactac gcatatcaag gttaacggtg ccaaccagac ttagttcgcc atcgaccagc
     6661 acgctcttgg tatgcagtaa cccgccttca aactgataaa ttttaacccc agcagcaaga
     6721 agttccgtaa agaatgcgcg actggcccag ccgaccagca tcgagtcatt ttttcgcgga
     6781 aggataatac tgacatccac cccgcgctgc gccgccgtgc aaatcgcatg aagtaaatca
     6841 tcgcttggca caaagtaggg cgtggtcatg atcaaatatt cacgcgccga ataagccgca
     6901 gtcaataatg cctggtgaat gagatcttcc ggaaagccgg ggccagaagc aattgtgtga
     6961 atggtgtgac cgctggcctg ttcaaacggc ataatattga catctggtgg tggcggcaga
     7021 atacgttttc cggtttcaat ctcccagtcg caggaataaa taatccccat cgcggtggcg
     7081 atggggcctt ccatacgcgc catcagatca atccattgcc ctacgcccgc atcttgtttg
     7141 aagtagcgag gatcgaccat attcatgctg ccggtgtacg cgatgtaatt atcgatcatg
     7201 atcatcttgc gatgttggcg caggtccata cggcgtaaaa acacacgcat cagattgacc
     7261 tttaaggctt cgaccacttc aataccggca ttacgcatta gctcgggcca cgggctgcgg
     7321 aaaaaagcca cactcccggc ggagtcgagt atcaatcggc aatgaatacc acgccgtgca
     7381 gccgccatta atgattcagc cacctggtcc gccatgccgc cgggctgcca gatataaaac
     7441 accatctcaa tattatggcg cgcgagctgg atgtcgcgga ttaacgcctg catcacatca
     7501 tctgactcgg tcatcagttg tagctgattc cctttgaccc cagcgatccc ctgacgacgc
     7561 tcgcaaagct tgaataatgg cgcagcgaca ctgctatttt cttcggcgaa gatatgctta
     7621 caggctttaa ggtcgttaag ccattttgcg gtggaaggcc acatcgctct ggcgcgctca
     7681 gcgcggcgtt tgcctaaatg gagctcgcca acggcaagat aggcaataat tccgactaac
     7741 ggcagaatgt aaataatcaa cagccaggcc atcgcggagg gaactgcgcg tcgtttcatt
     7801 agaatgcgta aagttacgcc tgcaatgagc aaccagtatc ccagaatggc caaccaactc
     7861 accaacgtat aaacggttgt catagattag aaatcctttt gaaagcgcat tgttatgagt
     7921 ttacgcatat ggattcatct ggcaaataaa aacgcgggaa aagcactggt cagcgtcggg
     7981 ggtgggggta taatgaccat tctgttattg catagagtag ttaacatgaa gcggagtaga
     8041 acggaagtgg ggcgctggcg catgcagcgt caggctagcc gacgtaaatc gcgttggctt
     8101 gaggggcaat cgcgccgaaa tatgcgtatc cacagcatca ggaagtgcat tctaaacaaa
     8161 cagcgtaact cgttattgtt tgcgatctac aatatctaaa tgtactgggc accattgcgg
     8221 tgcccaataa gtttactctt ccgtctttaa actctatttt tgcgccgatt ctttactgtc
     8281 agctttcgtt tcttgttcat cgttatcgct gaaaatacca tgacctgagt tcaacaacat
     8341 tgaaacgagc atatcattat taatctcttc accatttaaa actcgcgcca gaatttcgct
     8401 gccaaacagt tgcggtgaga gaatgatatc cggatgtacc atcttaatct tattcaggtt
     8461 tttgctatca ctgacggcga gaactgtttt gacatcactg ctcatatctt ttgccgagag
     8521 tacaacaaac gcgttatctg catcgttatc actcagcgcc agaatggccc ggcaacgatc
     8581 gattcccgct ttctttaata ctgaactgtc attactgtca ccggggataa catcagcgtt
     8641 atcgcctaag cgttgctcaa gttgcttgat atcatcttca ggcaagttgc tgataaccgt
     8701 tacgttttgt ccgcgttgat tcagttgcag gatcgtattg atggcgagaa tcgaatgtcc
     8761 gcaaacaata aaatgatctt tacgatgcat tgtatgattg tttcctttta caagtttgtt
     8821 gaatcccccg cggataagcg gaccaaaaat tgaggtcata gatgtggcaa aaacggtaat
     8881 gccggaaata atgaccgaaa tagtgaacaa tcgtgctgat tcagaaacag ggacaatatc
     8941 gccatagccg acggttgaca tggtttctat cgaaaaatag aacgcggtca tcaaactttc
     9001 tattcgcgga ttaaaacctt cgcttaaata aagcgcaccg taggttgagt aaaacagtaa
     9061 tgtcgtgaaa ctaataaatg caaaaattgt cccggctgcg gcgctactgt gggagaagtc
     9121 tttgcgcagt atcagcaaaa acaccagcgt aaaaatgcaa aatccaatac taaatttcag
     9181 ccagggataa aaatgcaggg tgtaaattag cgctatcaac aacaaaatga tactgattgc
     9241 ccatgcgagc tttgcccgga acagtaagcc aatggagttc aggacaagaa agacgccgag
     9301 cataaatagt ggggcgtttg ccagcaagga gagatccaac tctgaaaatg ctttgatatg
     9361 gaaaatatca agcaaattga ccgacatacc atagattgtt ttaaaaataa gcaatccatt
     9421 taaaaagacg gccagcgcga gaatgtcgtg ccgaagggtc acccataaat ttgttgctgt
     9481 ttgtttgaat gtagcccagt gactcactga tacctctgta accacaattt tgtttaatta
     9541 tcttagcgtg cacattttct gaacatacat gcagcgcgtg aatgtgttaa acaatagtag
     9601 atgactttat ccgcagtaat caaaggattt gagcgagcaa ctgtacctca ggaaaggtca
     9661 tttttcctga atatgctcac atcatataaa gaaatacaga taaagttatt atctgcttgt
     9721 ggtagtgaat gcactgaccg gctataagga aaggccaaac aagaacacgg ttgcaaaaac
     9781 cgtgccctta aatattgaat ctctattcag aacactttct taaatggttt cactgaaacg
     9841 tgttcataga ctcctgccgc tacgtacggg tcagcatcgg cccaggcctg agctgcttcc
     9901 agcgattcaa attcagcaat aacggttgag ccagtaaatc ccgcagcccc tggatcgtta
     9961 ctgtctaccg ctggcattgg accagctgtc aacaaacgac cttcatcatg cagtaactgt
    10021 aaacgtgcta aatgtgccgg acgaacggaa aggcgttttt cgaggctatc agctttgtct
    10081 tgagcgtaaa taacatacaa cacgggcaca actccttgtt cgggaaagtt gtaaagtacg
    10141 ttatttgaaa gggcgaagat ctgcaacgga aagatgatgt ctttgttaag accatgcata
    10201 aattaaggtc aattacgcca aaaatgacat tttcactgat cctgatcgtc ttgccttatt
    10261 gaatatgatt gctatttgca tttaaaatcg agacctggtt tttctactga aatgattatg
    10321 acttcaatga cccttgattt acctcgccgc ttcccctggc cgacgttact ttcggtctgc
    10381 attcatggtg ctgttgtggc gggtctgctc tatacctcgg tacatcaggt tattgaacta
    10441 cctgcgcctg cgcagccaat ttctgtcacg atggttgcgc ctgctgatct cgaaccgcca
    10501 caagccgttc agccgccacc ggagccggtg gtagagccag aaccggaacc tgagccgatc
    10561 cccgaaccac caaaagaagc accggcggtc attgaaaagc cgaagccgaa acctaagcca
    10621 aaaccgaagc cggtgaaaaa ggtacaggag cagccaaaac gcgatgtcaa acccgtagag
    10681 tcgcgtccgg catcaccgtt tgaaaatacg gcacccgcac gcctgacatc aagtacggca
    10741 acggctgcaa ccagcaagcc ggttaccagt gtggcttcag gaccacgcgc attaagccgt
    10801 aatcagccgc agtatccggc acgagcacag gcattgcgca ttgaagggca ggttaaagtt
    10861 aaatttgacg tcacgccgga tggtcgcgtg gataacgtac aaatcctctc agccaagcct
    10921 gcgaacatgt ttgagcgtga ggtgaaaaat gcgatgcgca gatggcgtta tgagccgggt
    10981 aagccaggca gtgggattgt ggtgaatatc ctgtttaaaa ttaacggcac caccgaaatt
    11041 cagtaagcag aaagtcaaaa gcctccgacc ggaggctttt gactattact caacaggtaa
    11101 ggcgcgaggt tttccttcag gatcaaccgc gacatactta aataatgctt ctgtcgcttt
    11161 atagcgttgc ccgattggtt cagacgctac ttttttcacc cacacttcaa tattaatgct
    11221 gaccgatgtc gtccctttct ggacacagcg tgcatagcag cacaccacat cgccgaccgc
    11281 aaccggccgt aagaaagtca ttccttcaac ccgcacagtc actacgcgac cgtgggcaat
    11341 ttctttcgcc agaatagcgc cgccaatatc catttgtgac attaaccaac caccaaagat
    11401 gtcaccattg gcattggtat cggcgggcat ggctaaagta cgtaaaacaa gatcgccctg
    11461 agggacgtta tgtgttgtag acatggtaaa accgacttaa aaggaaaatc acaggcgcga
    11521 tgctactatg attttcctga ggagaacaga ggagatacag cgccagcccc gaaggactgg
    11581 cagtctggtt aggatttatc ttcctgcggc atgtggcggt agatatagat accgcttaac
    11641 aatgtgaaga ttaaggtaag ggcggtcagg ccaaagactt taaagttgac ccaaatattt
    11701 tgcggcagcc agaatgcgat gtagatgttt gccagaccgc aaaggataaa gaaaacagcc
    11761 caagccagat ttagcttcga ccataccggt tgcggcagcg tgagttcttt acccagcatc
    11821 cgctgaatta gcggcttttt catcacccat tggctgacta acagggctcc cgcaaacaga
    11881 gcataaatga ctgtaacctt ccatttaata aactcatcat tgtggaagaa cagcgtcaag
    11941 ccaccgaaga cgaccaccag aacaaaagtg atcagggcca tcttctcaac cttacgaaag
    12001 cgaacccagc tatagataag cacaatcgcc gtggcgacga tcagcgccgc agtagccgca
    12061 tagatgtcat aaatcttgta aaacgcgaaa aagacaacca gcggtaaaaa atcaagaaac
    12121 tgcttcattt tacgattccg taatcaaatg cttaaggtta ttggcgaatc aacatataca
    12181 ggcggaacag atagatgagc aatatggcag aaatcaggtt gctcaaggtg ttcgccagta
    12241 cggcaccaat ttccggggtt aatgcggcaa aagaagaggc aaaaagcagc aacagtgttt
    12301 ttgccagcaa ccagctcagt actgcgggtg ccaccagacg catattcgcc caagtcagcc
    12361 gcatactgct acgcatcgag gcaaaaatgc ccattttgtc ctgtaccagc atcaccggtg
    12421 ccagggccag taaaatggcc atgataattc ccggaacgac caccagcatg atgccaatct
    12481 gtactaaaag ggtagtcaga aaaatcagaa taaataactt gggcaatatc ggcgcactgg
    12541 caccaatagc tcgcagcgca ctgactctct gacccgcaga caccagctgg ataattaata
    12601 ttacgcctcc ggcgagaatg gcgttaccga ttaatcctga aaaagtggac gccgctgaag
    12661 cctgcagcaa aatttgttgc tgttccggtg acatattctg aaccaggtcg aacaacccac
    12721 tactgccgct aacgggcacg ccgtcattga gctgcgcaag ctgtgcatca ctgggtgaga
    12781 aaacatgccc taacaccact gtgataaacg cacatagcaa cgataccaac agaatggtca
    12841 taaattgatt acggaagaaa tttccggtgt cacggtatac ggactgcgcc gtgatagaca
    12901 tgcactctcc ttgagtttta tgcaggtgtt aattagcggg caattgtacc ctggttaagc
    12961 ctcgggtggc agcatcaggt cttgtatgga aaagcatatc tttgtaaagc ggaggtaatt
    13021 gctggccttc aggcgtgggc aaaccgcggc aagtggcgat cgagttaata tttgcgtaga
    13081 gaaaatattt aaaaattgat ttaaatcaca ttaaccagga ttctcaatgc aacttctaaa
    13141 ttaatccaga tcaataaatg gtgaattatc atatgtaatg tgatctatgt aggatcattt
    13201 gttactccaa tgtaggtata ttcgtcacgt ttttataacc ataacgacgg agcggatatg
    13261 aaaaagttaa cagtggcggc tttggcagta acaactcttc tttctggcag tgcctttgcg
    13321 catgaagcag gcgaattttt tatgcgtgca ggttctgcaa ccgtacgtcc aacagaaggt
    13381 gctggtggta cgttaggaag tctgggtgga ttcagcgtga ccaataacac tcaactgggc
    13441 ttgacgttta cttatatggc gaccgacaac attggtgtgg aattactggc agcgacgccg
    13501 ttccgccata aaatcggcac ccgggcgacc ggcgatattg caaccgttca ccatctgccg
    13561 ccaacactga tggcgcagtg gtattttggt gatgccagca gcaaattccg tccttacgtt
    13621 ggggccggta ttaactacac caccttcttt gataatggat ttaacgatca tggcaaagag
    13681 gcggggcttt ccgatctcag tctgaaagat tcctggggag ctgccgggca ggtgggggtt
    13741 gattatctga ttaaccgtga ctggttggtt aacatgtcag tgtggtacat ggatatcgat
    13801 accaccgcca attataagct aggcggtgca cagcaacacg atagcgtacg cctcgatccg
    13861 tgggtgttta tgttctcagc aggatatcgt ttttaattcc acaaaaaaac gaccccgtaa
    13921 tatacggggt caataaggac atggcataaa gtgtattatt tcttcgcttc tacgccgtca
    13981 gtttcagagc gaattaaaaa tttctcagtt gtttgcggaa tatgctgaat cagccagtcg
    14041 gccatttgct tttcctcatt taaaatcgct tcgatggttg gaattgaagc tgtatcaccg
    14101 gcattttttg ctgctgctaa tagtgaggtg taacaggcga tttcaaattg ctcgaagaca
    14161 tatccgctaa tagagccttt gactatttca tcagaaggga atataccacc gattgactgc
    14221 ccaagcgcag ccattttact catggaatct ttaatgactg aacgtgaaat gtcattacga
    14281 tcaagaatag tttccagttg aacaatctgg tttttggttt cactaagatg ttgttcaata
    14341 cgagcgcgta gttcaggata attatctata cggctggcca tggattcaag catagattcg
    14401 gcttgctttt ccattgcgtg ggcgtcacgt aaccagtcat gataatgttc aatacgattc
    14461 atgaaaatgt cctctttata aaatgactaa aagttaaagt tcatatttca ggctttattt
    14521 tcggctttct tatttacgtt attaagggcc agatcagtca gtttgatgtc ggtggccttt
    14581 tcttcttcca gggtctcttt cagaagcttc gctgctttac gataacctaa ttgttcagcc
    14641 agcgtcgcta atgtcccgta actggcaatc tcataatgct cgactttctg tgctgcggca
    14701 atcagtgcgg catcacgcac ttcgtttttc tcggtacttt cgatgacctc attagcttct
    14761 tcaataagac cttccattgc cacacatttc atgcgcttaa ttttcagatt cgattctgat
    14821 tccacaactt gatcaatacg ttcaatctgt ccatgtgttt cctcgaggtg cgcatgaaaa
    14881 gcctgactta atttttcgtt tgatgttgct cttgcgagtt ttgccagtgc ccgggttaat
    14941 tgtttttctg cgctgtaggt atctgaaagc aggtgaataa atacatcttc aatggtcttc
    15001 atattcatat ttttctccag tgaaatcact gcgagccata cggcccgcag atgcaattat
    15061 ctttatcagc tgatattaat aattaacggt gaatcaggat ttgcctgatt tattaccacc
    15121 gctttgttga ccgccttttt tacccgcttc agatgcgcgt tgcggatcat ttttaaaatt
    15181 accgccgcta tgctgaccgc ctttacggcc tgcgtcggat gccttctcac ggtcttcggc
    15241 gaaatttcct gaaccaccac gatgttcggc catgttattt ctcccgttgc gttgcattgt
    15301 ttcattaata tgagtgttgt gtgtcgacac tcattaaaat tagtcgctaa tgagaattag
    15361 tcaaattaag cgcaacgaga agatagaggg aaaatatatt ttgaggaaca ttctggatat
    15421 attaacaatt acctgaggaa taagtgactt agagaaaatt attgatttta ctggtgttat
    15481 gtt
//
LOCUS       DBANUU010000100        15387 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000100 DBANUU010000000
VERSION     DBANUU010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.100"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(128..934)
                     /gene="trpA"
                     /locus_tag="ACIE68_003304"
     CDS             complement(128..934)
                     /gene="trpA"
                     /locus_tag="ACIE68_003304"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFX5909570.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(934..2127)
                     /gene="trpB"
                     /locus_tag="ACIE68_003305"
     CDS             complement(934..2127)
                     /gene="trpB"
                     /locus_tag="ACIE68_003305"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFX5909571.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2139..3500)
                     /gene="trpCF"
                     /locus_tag="ACIE68_003306"
     CDS             complement(2139..3500)
                     /gene="trpCF"
                     /locus_tag="ACIE68_003306"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFX5909572.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3501..5096)
                     /gene="trpD"
                     /locus_tag="ACIE68_003307"
     CDS             complement(3501..5096)
                     /gene="trpD"
                     /locus_tag="ACIE68_003307"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFX5909573.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5096..6658)
                     /gene="trpE"
                     /locus_tag="ACIE68_003308"
     CDS             complement(5096..6658)
                     /gene="trpE"
                     /locus_tag="ACIE68_003308"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFX5909574.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6679..6772)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6749..6793)
                     /gene="trpL"
                     /locus_tag="ACIE68_003309"
     CDS             complement(6749..6793)
                     /gene="trpL"
                     /locus_tag="ACIE68_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFX5909575.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6931..7812
                     /gene="rnm"
                     /locus_tag="ACIE68_003310"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6931..7812
                     /gene="rnm"
                     /locus_tag="ACIE68_003310"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFX5909576.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7809..8429
                     /gene="yciO"
                     /locus_tag="ACIE68_003311"
     CDS             7809..8429
                     /gene="yciO"
                     /locus_tag="ACIE68_003311"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFX5909577.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8530..9402
                     /gene="rluB"
                     /locus_tag="ACIE68_003312"
     CDS             8530..9402
                     /gene="rluB"
                     /locus_tag="ACIE68_003312"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFX5909578.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(9442..10032)
                     /gene="cobO"
                     /locus_tag="ACIE68_003313"
     CDS             complement(9442..10032)
                     /gene="cobO"
                     /locus_tag="ACIE68_003313"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFX5909579.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(10029..10787)
                     /gene="yciK"
                     /locus_tag="ACIE68_003314"
     CDS             complement(10029..10787)
                     /gene="yciK"
                     /locus_tag="ACIE68_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFX5909580.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            11007..12056
                     /gene="sohB"
                     /locus_tag="ACIE68_003315"
     CDS             11007..12056
                     /gene="sohB"
                     /locus_tag="ACIE68_003315"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFX5909581.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(12092..12343)
                     /gene="yciN"
                     /locus_tag="ACIE68_003316"
     CDS             complement(12092..12343)
                     /gene="yciN"
                     /locus_tag="ACIE68_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFX5909582.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            12723..15320
                     /gene="topA"
                     /locus_tag="ACIE68_003317"
     CDS             12723..15320
                     /gene="topA"
                     /locus_tag="ACIE68_003317"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFX5909583.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         3727 a         3914 c         4135 g         3611 t
ORIGIN      
        1 tgacgtaagt tgacgttcga ccggggtaag tgaaacggta aaaagataaa tattaaatga
       61 atttaggatt tttccggctt cattaaagaa agttaaaatg ccgccagcgg aactggcggc
      121 tgtgggatta actgcgcgtc gccgctttca tcggttgtac aaaaactttc agtgccgcca
      181 gcattttctc tggctcatta atatgttgct cgatgatttt aacaatggcc gaaccagaaa
      241 tcgcgcccgc agctcctgca tcaatcgctg cttttacctg atccggggcg gaaataccaa
      301 acccctgcaa tggaggtgca gcgttgtact ctttcagctt cgcaaccaga tgattgaggg
      361 gtaacgcggc gcggttttct gcgccggtca cgcccgctcg tgacagcaaa taggtgtaac
      421 cacgaccgta agaggctatc tggcgcagca ggtcgtcatc ggcatttggc gggcagatga
      481 agataggtgc gacattatgg cgcaacgcgg cctggcggaa gggcgcggac tcttcaattg
      541 gcacatcggc aaccagcacc gaatcgacgc cgactttctc gcactgggca taaaactcat
      601 caatgccttt gttaaacacc agattggcat acatcaacag gccaatggga atggtcgggt
      661 gtttctggcg aatcagtgcc agcatttcaa aacattgtgc cggagtcaca cctgccgcaa
      721 aggcgcgcag agtggcgttt tgaatggtcg ggccatccgc cagtgggtcg gagaagggga
      781 tacctaactc cagcgcgtca gcaccggctt caattagcgt atcgataatt ttcaatgact
      841 gctcaatgcc cggatcaccg agcgtgacga aaggaacgaa tgcgccttct ttgcgctcct
      901 tcaactgggc aaacagagat tcgtagcgtt ccatcagatt tcccctcgtg ctttcaaaat
      961 atcgtgaacg gtgaagatgt ctttatcgcc gcgaccggaa aggttaacca ccagtagctg
     1021 ctctttatcc gggttttcgc gcatcatttt caacgcatgg gccagggcgt gggaggattc
     1081 cagcgccggg atgatccctt cgtgcaggca cagcgttttg aaggcttcaa gggcttcatc
     1141 atcggtaata gacacgtaat cagcgcgtcc agtgctgtta agatacgcgt gttgtgggcc
     1201 gacagacggg aaatccagtc cggcggagat ggagtaagat tcttcaatct gcccgtcttc
     1261 ggtttgcatc atcggcgctt tcataccgaa atagatgccc acgcgaccat gttttaacgg
     1321 tgcgccgtgc tcgccagttt cgataccgtg accacctggc tccacaccaa tcaggccgac
     1381 gttggtttca ttgatgaaat cagcaaacat gccgatggca ttcgaaccgc cgccaacaca
     1441 ggcgataacg gcatccggca ggcgaccttc tctttccaga atctgcgctt tggtttcttc
     1501 gccaatcatc cgctgaaact cacgcacaat ggtcggataa ggatgcgggc cagctgcggt
     1561 gcccagcata tagtgcgcgg tttcgtaact accggaccag tcgcgcagcg cctcgttaca
     1621 ggcatctttc agcgtcgcgg aaccgctatg caccgggatc acttccgcac ccattaagcg
     1681 catacgaaaa acgttaggcg actggcgttc tacgtctttg gcacccatat aaatacggca
     1741 tttcaggccg agcagggcgc tggcaagggc cgacgccacg ccatgctgac cggcaccggt
     1801 ttcggcgatg atttcggttt tacccatccg cttcgccagt aaagcctgac cgagcacctg
     1861 gttagtttta tgcgcgccgc cgtgcagcaa atcttcacgc ttgagataca gagtggtgtt
     1921 cgtcccggct gtaatgttct ggcatttggt cagcgcggtt ggacgcccgg catagttttt
     1981 cagcaggtcg ttgaactgag cctgaaattc aggatctttt tgcgcactga caaaagcttc
     2041 ttccagctgg cgcagagcag gcatcaggat ttgtggcacg tacatgccgc caaactcacc
     2101 aaaatagggg ttaagtaatg ttgtcattgt tcctttcctt aatatgcgcg cagcgtctgg
     2161 aaaaccgagg ccaaaagacg tgcgtctttg atgcccggtt gcgactctac agcagaatta
     2221 aaatcaagtc cggcgcagcc ggtttgtgcc gcttccacgc agttatctgc gcctaagccc
     2281 cccgccagca gaacgttgcc aagcgattga ccatttaata gtgaccagtc gaaacgttgc
     2341 ccgcttccac cctggccgtt gtctaaaaca tatttatcaa cgtgctgaag ctcgcgggcg
     2401 ggcagggttt caccgacgct taatgctttc cagatggcaa cgtgtgctgg cagagcttca
     2461 cgcagcgtat cgatatacag ctgatcttca ttaccatgca gttgcactgc cgccagcgat
     2521 aacaccttag ctttgtccac cacatcggca atatcgtgat tgcggaacac gccaacatac
     2581 tgcaacggtg ccgcagccat cacttcctgc gcctgttcaa cgttgacgca acgcggtgat
     2641 gtcgcaacaa aaatcaaccc accgtaaatc gcgcccgcgt cataagctgc tttagcatct
     2701 tgcccacgcg tcaggccaca tactttattc tcacccagca acacccggcg cacggcggcg
     2761 tgcaaatcgt catgggccat caacgccgaa ccaatcagaa aaccgttagc gaagtggctt
     2821 aactcgcgca cctgagcgta agtattgatg ccggattcgc tgattaccgt tacgttgtgc
     2881 cccagtttcg gcgcaagctc gcgggtacgg ttgagatcaa tcgacaaatc acgcagatcg
     2941 cggttgttga tgccaacgac ctttgccccc aatgcaatgg cacgctccag ttcctcttca
     3001 ttactgactt cggtcagcac acccatctcc agactgtggg cgacggcggc aagctggcga
     3061 tattgttcgt catccagtac tgaaagcatt aataagcagg catcagcctg gtaatagcgc
     3121 gccagataga tctggtaagg atcgataatg aagtctttac ataaaatcgg ctgcggggcg
     3181 atttggctga cgatggggag gaaatcaaag ctcccctgaa aatatttctc atcagtcagc
     3241 accgaaattg ccgaagcgta atgtttataa atggcggcaa tgcgtgctgg atcgaaatca
     3301 tcacggatca cgccttttga cggcgacgct ttcttgcact ccagaataaa cgccgtgcgc
     3361 gcaccctgta gcgcatcata aaaatgtcgc gtgctcggct gaacctcatt ctgaaaactg
     3421 gccagcggtt gctgctgttt gcgggtttct acccaaatcg ccttgtctgc gacgattttc
     3481 gctaaaacgg tttgcatcat ttaccctcgt gccgccagcg cggtaactct gtcgtaagcg
     3541 gaaccactgc gcagtacctc aagaacggtt tgcgcattgg cttgcagatc ttcatggcca
     3601 tgcaggcgca ttaacatggc gacgttcgca gcgacggctg cttcatgggc ggcgtcgcct
     3661 ttaccttgta acaaacgtgt taaaatgtca cggttttctt ccggtgttcc gcctgccagt
     3721 tgctcctggt ggtagggtgt caggccaaaa tcttcagcgg tcaattgata gctcttaatt
     3781 tcgccgtcat gcagctcggc aacgattgtc ggcgcgtgta atgaaacttc atccatcccg
     3841 ccgctgtgca ccaccgccgc gcgttgatac cccagcacgc gcaaggtttc ggcaatcggc
     3901 agcaccagtt ccggactata aacaccaatt aacgccagcg gcggatgcgc cgggttaatc
     3961 aatggcccca gcacattgaa cagggtgcgg gtttttagtt gctggcgaac cggcattgca
     4021 tggcgaaatc cggtgtgata tttcggtgca aagaggaaac atacgcctaa ctcatccagc
     4081 gcctggcgcg atttatcggc gttcatatca agattaatac cgaacgccgc cagcagatcg
     4141 gacgaaccag atttactgga gacgctacgg ttgccgtgtt tcgccacttt cagcccacag
     4201 gccgcggcga caaacgcact ggcggtagaa atattgatac tgttgctgcc gtcaccgcca
     4261 gtaccgacga tatcagcaaa cagataatcc gggcgcggga acggcgctgc gttttccagt
     4321 agcgcggttg ctgccccggc gatctcgttc gggtgctcac cgcgaatttt catgctcacc
     4381 agcgccgccg ccagttgttc cggcttcagc tcgccacgca ccaccgctga aaacagctgg
     4441 tggctttctt gttggctaag cgtctgcgcc tgatacagtt tttccagaat cggttgcagc
     4501 gtgttggctg gctctagttt ctgctgcgcc caggccagcg tttgttccag caggcgagcg
     4561 ccctgggtgg tgagaatgga ttccggatgg aactggaatc cacaaacgcg atccgcatcg
     4621 tgacgtactg ccatcaccat gccattaaaa tgggcgttga tggttaaacc ggccggaatg
     4681 ttactgccaa ccagcgagtg ataacgcgcc accggcagcg ggtttgttaa tccggcaaac
     4741 atcgcctgac cgtcatgttc aatgctcgac gctttaccgt gaagaatttc gcccgcctga
     4801 ccgacatagc ccccgtaagc ttcgacaatt gcctgatgtc cgaggcaaat gccaataatg
     4861 ggcagcttgc cacgcaagcg ggtgaggagt tccggcatac aaccggcttc gctcggcaca
     4921 ccggggccag gagaaagcat cagtaccgga ttgctcatgg tcgccaggcg ttcaattaag
     4981 gtctgcgccg gaatatggtt gcggtaaatc accacgttat gcccattgct gcgcaactga
     5041 tctgccaggt tgtacgtaaa agagtcgata ttatcgagca gcagtatgtc agccatcaga
     5101 aagtctcctg tgcatgatgc gcggtggcaa tagcgcgcag tacagcgcgg gctttattac
     5161 gggtttcgtc ggcttccgac tgcggaatag aatcaaggac tacgccagca ccggcttgca
     5221 cggtggcgat accgttttcc accagcgccg agcggatcac aatgcaggtg tcgagatcgc
     5281 cgtgcgcggt gaaataacct accgcgccgc cgtagctgcc gcggcgacga ccttccgcct
     5341 cggcaattaa ctgcatggcg cgcactttcg gcgcaccgct taacgtcccc atattcatac
     5401 aggcgcggta agcgtgtagg gcgtcgagat cgtggcgcag ctcaccgacc acgcgggaga
     5461 ccaggtgcat cacgtaagag taacggtcaa ctttggtgag gtcggcgacg tagcggctgc
     5521 cgggggtgca aatgcgtgcc agatcattac gggcaagatc caccagcatc agatgttcag
     5581 aaagctcttt atgatcggta cgcatttcca gttcgatgcg gctgtcgagg tctctgtcca
     5641 gcgagccatc ggcacgacga ccgcgtggac gtgttccggc aatcgggtag atctcaatct
     5701 ggcggctggt ggcgtcatac ttgagcgaac tttccggcga cgcgccaaac agggtgaaat
     5761 cattatcctg cataaaaaac atgtacgggc tgggattact ctttttcagc acgtaatagg
     5821 ccgccagcgg tgacgggcag ggcagagaga aacggcgaga tggcaccacc tggaaaattt
     5881 ctccagcgcg aatcgctttt tgcaacaaac gcactacgcc accgaactct tcatcgctct
     5941 gattacattc acaacgcata tgcggcacgg aaaccaccgg cagcggcggc gcggcttcgg
     6001 tcagttgctg acgtagttcg ttcaggcgag cagtgagacg ttgtttttct tcttcattcg
     6061 gagcaaacag gctggcctga atacgggtgc tttttttctg atggtcaatc accatcagag
     6121 tttcagcgag ataaaaacag aaatcagggc agttattttc cgctgacagt tgcggtaaat
     6181 cttcaaatcc cgccacaagg tcataagaga acaggccgcc gaagaacatt gcttctcgtt
     6241 cttccttcgg tacattcaac agattctgca ataaacggaa agcgtcaaaa accgaaaggg
     6301 agcataagcg agcgtcttca tccagcagtg gactgacagg ggggaagcgc agcacacggc
     6361 agtttggtaa ttgttcattt tccacacccg caggcagggc gttatccagt agtgtcagca
     6421 gggcttcgcc gttgccggaa agtgcctgga ttgtgacagt gtcacctaaa gctgtaatgc
     6481 gcagcgcact gtctaccagc agcaggcttt ttaaatcatc tttgctgtcg atatctgcgg
     6541 attccagcag cagcgttgcc ggacgatccc cacacaactg gtgaaaaagc gcggtgggat
     6601 tgtcgcgata agcgccttcg caggttagca gttcgagagt cggtttttgt gtttgcattg
     6661 ttattctcta attttgttca aaaaaagccc gctcattagg cgggctaggt atctgattgc
     6721 tttacgcatg gtgaatacac tgcccgtttc aggaagtgcg ccaccaacct ttcagtacga
     6781 aaattgcttt cattgtcgat acccttttta cgtgaacttg cgtactagtt aactagttcg
     6841 atgattaatt gtcaacagct catttcagaa tatttgccag aaccgttatg atgtcggcgc
     6901 aaaaaacatt atccagaacg ggagtgcgcc ttgagcgaca cgaattatgc agtgatttac
     6961 gacctgcaca gccataccac agcttccgat ggctgcctga cgccagaagc attggtgcac
     7021 cgtgcagtcg agatgcgcgt cggcaccctg gcgatcaccg accatgacac cacagctgca
     7081 atcgcgccag caagagagga aatttcacgt tcgggactgg cgttgaatct tattcccggc
     7141 gtggaaattt ccacggtctg ggaaaatcat gaaattcata ttgtcgggct gaatattgat
     7201 attactcatc cgctaatgtg tgagttcctc gcgcagcaga cagaacggcg caatcagcgg
     7261 gcgcagctga ttgccgaaag acttgaaaaa gcgcaaatac ctggggcgct ggaaggcgca
     7321 caacgactgg cgcagggcgg cgcagtgacg cgcggtcatt ttgcgcgttt ccttgttgag
     7381 tgcggcaaag ccagttcaat ggcggatgtc tttaaaaagt atctggcgcg cgggaaaacc
     7441 ggatacgttc cgccacagtg gtgtacaata gaacaagcta ttgatgtcat tcatcattct
     7501 ggcggtaagg cggtgctagc tcatcctggg cggtacaatc ttagtgctaa atggctgaaa
     7561 agattggttg cgcattttgc cgaacaccac ggtgacgcga tggaagtcgc gcagtgccag
     7621 caatcgccca atgaacgtac ccagctggcg gcccttgcgc gtcagcatca tttatgggca
     7681 tcacaaggat ctgattttca tcagccatgc ccgtggatcg aactgggtcg taaactctgg
     7741 ctgcccgcag gcgttgaagg cgtctggcag ctatgggaac agccgcagaa caccacagag
     7801 agggaattat gagccagttt ttttatattc atcctgataa cccacagcaa cgtctgatca
     7861 accaggcggt ggagatcgtg cgtaaaggcg gggtgattgt ttatccaact gattccggct
     7921 atgcgctcgg ctgtaaaatt gaagataaaa acgcgatgga gcgtatttgt cgtattcgcc
     7981 agctgccgga cggtcacaac tttaccctga tgtgtcgcga tctttctgaa ctctcgacct
     8041 attcatttgt tgataacgtt gcgtttcgtt taatgaaaaa caacacgccg ggcaactata
     8101 ccttcatctt gaaggggacg aaggaagtgc cacgccgtct gttgcaggaa aaacgcaaaa
     8161 ccatcggtat gcgtgtgcct tctaacccga ttgcgcaggc gttgcttgaa gcactgggcg
     8221 aaccgatgct ttccacttcg ctaatgctgc caggcagcga atttaccgaa tccgatcccg
     8281 aagaaattaa agaccgtctg gaaaaacagg tggatttgat tatccacggc ggctatctcg
     8341 gacagaaacc gacaacggtt attgatctta ccgatgatac gccggtcgtg gtgcgtgaag
     8401 gcgtaggtga tgtgaagcct ttcttataag tgcgtaactc tgtatactac gcggccttaa
     8461 aatggcgcta tacggcgcca ttgattctcc tgttcgacgc ctgtgaaggc gacaccaaaa
     8521 ggaagctcta tgagcgaaaa attacagaaa gtgctggccc gcgctggcca cggctcccgc
     8581 cgtgaaatcg aatctattat tgaagccggt cgtgtgagtg ttgatggcaa aattgctaaa
     8641 ctcggcgatc gcgttgaagt tacccctggg ctgaaaattc gtatcgatgg tcatctgatt
     8701 tcggtacgtg agtccgctga acaaatttgt cgcgttctgg cctattacaa accggaaggt
     8761 gagttgtgta cccgtaacga tccggaaggg cgtccgacgg tgtttgaccg tctgccaaag
     8821 ctgcgtggcg cacgctggat tgccgtaggt cgtctggacg ttaacacctg tggtctgctg
     8881 ctgttcacca ccgatggtga actggcaaac cgtttaatgc atccaagccg tgaagttgaa
     8941 cgtgaatacg ccgtgcgtgt atttggtcag gttgacgacg cgaaactgcg tgatttgagt
     9001 cgtggcgtgc aactggaaga tggtccggca gcttttaaaa ccatcaagtt cagcggcggc
     9061 gaagggatca accagtggta caacgtgact ctgaccgaag gccgtaaccg tgaagttcgt
     9121 cgtctgtggg aagcggttgg tgtgcaggtg agccgcctga tccgtgttcg ttacggtgat
     9181 atcccactgc caaaaggtct gccacgcggt ggctggacgg agctggacct cgcccagact
     9241 aactatctgc gcgaactggt ggagcttccg ccagaaacca gctctaaagt cgctgtagaa
     9301 aaagaccgtc gtcgcatgaa ggctaatcag attcgtcgtg cggtgaaacg tcacagtcag
     9361 gtgagcggcg gtcgtcgttc tggcgggcgt aataacggtt aatcaaagta tgccggacgt
     9421 catatccggc atttttacag attaataatc aatccctatt tgcgccttaa caccggcatc
     9481 aaacgcatgt ttgatggggc gcagctcact taccgtatcc gccagttcaa gaatatcccg
     9541 atgacagcct cgacccgtga taatcaccgt ctgttgatgt ggacgttcat ttaacgcctg
     9601 caccacttct tccagtggca aatagtcata cgccaccata tacgtcagtt catcaagcaa
     9661 aaccatatcc agtgaggaat cagcaagcat ccgctttgca tgttgccaga cttcgcggca
     9721 ggcggcggta tcagactcgc ggttttgtgt atcccaggta aagcccgttg ccatcacctg
     9781 aaactcaacg ccatgtggct ccagcagatt gcgttcgcca ttaggccagg tgcctttaat
     9841 aaactgcacg acgcctactt tttttccgtg accaactgcg cgtgttgccg taccaaatgc
     9901 cgcggtggtt ttgccttttc cattgccggt aaagacgatg ataataccgc gttcatcctg
     9961 ggcctgggcc acacgagcat ctactttttc tttcactcgc tgctgacgct gttggtagcg
    10021 ttcatcactc attgggaaat tcctggttta cggcccggtt gggcgtcaaa ggtcatgccg
    10081 gttttacggc ggctgtcatc gcccatcagc cagaggtaga gcggcatgat atcagcgggt
    10141 gttttaagtt tctgtggatc ttcggtcggg aaggcgctgg cacgcattgc ggtgcgcgta
    10201 ccgcctgggt taatgcagtt gacgcgcagg cgctgctgat attcatcggc cagtacctgc
    10261 atcatccctt cggtggcaaa tttcgacgct gcatatgcgc cccagttggc tcgtccctga
    10321 cgtccaacgc ttgatgaagt aaagaccagt gaaccggcgt ccgatttgag taataaagga
    10381 agcagtgcct gggtgagcat aaaggtggca ttaacgttga cctgtatgac gtcctgccag
    10441 acctgtggat tttgttcgct cattgggcaa acatcgccga gcaatccggc attatgcaaa
    10501 acaccatcca gacgcggata attaacggta atgcgctgtg ccagttgttg gcaatcttcg
    10561 gacgtgcagg tcagcaaatc gagaataaac cactgtggct gacgtccagt ttcttcgttt
    10621 atgtggctgg ctacctgacg taatttttct tcattacggc ccaacagaat cactgtcgca
    10681 ccatagcgtg catacgtcat cgcggcttca cggccaatcc catcgctggc tcccgtcacc
    10741 aggataatgc gatcattgag taaatcttgt tttggctggt aatgcatggc tactcctcaa
    10801 cgacgttgtc tgtcgtgctg ccagtgtact ttatgacttt ggggctttat gcctgaaaca
    10861 gaagggcatt tcaatcagcc tggggcgata acaccgcaaa atggatactt tgtcatactt
    10921 tcgctgcaat aacatctctg cgagacggct taacatgcct gttgtaaact gtgagccaaa
    10981 gcgttgttta accaaggtgg ggactcgtgg aattgttgtc tgaatatggt ttgtttttgg
    11041 cgaaaatcgt taccgttgtg ctagcgattg cggcgattgc cgccattatt gtcaatgttg
    11101 ctcaacgtaa taaacgccag cgtggcgagt tacgggtcaa caatctcagc gaacagtata
    11161 aggagatgaa agaagaactg gccgcggcgc tgatggactc acatcagcaa aaacagtggc
    11221 acaaagcgca gaagaaaaag cacaagcaag aagcgaaagc agcaaaagcg aaagccaaac
    11281 tgggcgaggt ggcaactgac agtaaacctc gcgtctgggt gctggatttt aaaggcagca
    11341 tggacgccca tgaagtgaac tcgctacgtg aagagataac ggcggtactc gcagcattca
    11401 aaccgcagga tcaggttgtg ctccgtctgg aaagccctgg tggcatggtg catggttacg
    11461 ggttggcggc ttcgcagctg cagcgtctgc gtgataaaaa cattccttta actgttacgg
    11521 tagacaaagt cgctgccagc ggcggttaca tgatggcctg tgtggcggac aaaattgttt
    11581 ccgcaccgtt tgctattgtg ggttccattg gggtggtggc gcaaatgccc aactttaacc
    11641 gcttcctgaa aagcaaagat attgatatcg aactgcacac tgccgggcag tataagcgta
    11701 ctctgacgtt gctgggtgaa aataccgaag aagggcggga gaaattccgc gaagagttga
    11761 acgaaacgca tcagttgttt aaagattttg tgaagcgtat gcgtccgtct ctggatattg
    11821 aacaggtggc aacgggtgaa cactggtacg gacaacaggc ggtagagaaa ggcctggttg
    11881 atgaaatcaa caccagtgat gaagttattc ttagcctgat ggaaggccgt gaagtggtta
    11941 atgtacgcta tatgcagcgt aaacgactca ttgaccgatt caccggcagc gcggcagaga
    12001 gcgccgatcg attgttgcta cgctggtggc agcggggtca aaagccattg atgtaaaaga
    12061 caaacgcgag gctaagacct cgcgttttgc tttaatcaac cagatgatat ttttctgaaa
    12121 gcacatgggc caggtgtttg aacatattaa acaccgcggt gcttttggct gttggcaatc
    12181 cttgttcatc taaaaagtag tcgccggtaa ataccagcac gccattacgc tgcgtgacgc
    12241 cggtggcttc aatccctgcg agcgtatcct catgctcacg aatgattttg ttggcctctt
    12301 tcaacagcgt ttcgcggtcg atgggttgtg tctctttgtt cattatttac tccttaaaca
    12361 aggacattag tctacgccag gcatggcttg cagacaaata taccacgctg gtggcaagag
    12421 cgccttactg gtaactttgg attttgcatg ctaataaagt tgcgtatcgg attttatcag
    12481 gtacagtgtg acgctttcgt caatctggca atagatttgc ttgacattcg accaaaattc
    12541 cgtcgtgcta tagcgcctgt aggccaagac ctgttaactc agtcacctga attttcgtga
    12601 acagagtcac gacaaggggt tgatatccgc agagaacgag tccatatcgg taactcgttg
    12661 ccagtggaag gtttatcaac gtgcgacgca ttcctggaag aatcaaatta ggtaaggtga
    12721 atatgggtaa agctcttgtc atcgttgagt ccccggcaaa agccaaaacg atcaacaagt
    12781 atctgggtag tgactacgtg gtgaaatcca gcgtcggtca catccgcgat ttgccgacca
    12841 gtggctcagc tgccaaaaag agtgccgact ctacctccac caagacggct aaaaagccta
    12901 aaaaggatga acgtggcgct ctcgtcaacc gtatgggggt tgacccgtgg cacaattggg
    12961 aggcgcacta tgaagtgttg cctggtaaag agaaggtcgt ctctgaactg aaacaactgg
    13021 ctgaaaaagc cgaccacatc tatctcgcaa ccgaccttga ccgcgaaggg gaagccattg
    13081 catggcacct gcgggaagtg attggtggtg atgatgcgcg ctatagccga gtggtgttta
    13141 acgaaattac taaaaacgcg atccgccagg catttaacaa accgggtgag ctgaatattg
    13201 atcgtgttaa tgcccagcag gcgcgtcgct ttatggaccg cgtggttggg tatatggttt
    13261 cgccgctgct atggaaaaag atcgctcgtg gtctgtctgc cggtcgtgtg cagtcggtgg
    13321 cggttcgcct ggtggtcgag cgtgagcgtg aaattaaagc gttcgtgccg gaagagttct
    13381 gggaagtcga tgccagcacg accacgccat ctggtgaagc gttggcgttg caggtgactc
    13441 atcagaacga caaaccgttc cgtccggtca acaaagaaca aactcaggct gcggtaagtc
    13501 tgctggaaaa agcgcgctac agcgtgctgg aacgtgaaga caaaccgaca accagtaaac
    13561 ctggcgctcc ttttattacc tctacgctgc aacaagctgc cagcacccgt cttggatttg
    13621 gcgtgaaaaa aaccatgatg atggcgcagc gtttgtatga agcaggctat atcacttaca
    13681 tgcgtaccga ctccactaac ctgagtcagg acgcggtaaa tatggttcgc ggttatatca
    13741 gcgataattt tggtaagaaa tatctgccgg aaagtccgaa tcagtacgcc agcaaagaaa
    13801 actcacagga agcgcatgaa gcgattcgtc cttctgacgt caatgtgatg gcggaatcgc
    13861 tgaaggatat ggaagcagat gcgcagaaac tgtaccagtt aatctggcgt cagttcgtag
    13921 cctgccagat gaccccagcg aaatatgact ccacgacgct gaccgttggt gcgggcgatt
    13981 tccgcctgaa agcacgcggt cgtattttgc gttttgatgg ctggacaaaa gtgatgcctg
    14041 cgttgcgtaa aggcgatgaa gatcgcatct taccagcagt taataaaggc gatgctctga
    14101 cgctcgttga acttacacca gcccagcact ttaccaagcc gccagcccgt ttcagtgaag
    14161 catcgctggt taaagagctg gaaaaacgtg gtatcggtcg tccgtctacc tatgcgtcga
    14221 tcatttcgac cattcaggat cgtggctacg tgcgagtaga aaatcgtcgt ttctatgcgg
    14281 aaaaaatggg cgaaatcgtc accgatcgcc ttgaagaaaa tttccgcgag ttaatgaact
    14341 acgactttac cgcgcagatg gaaaacagcc tcgaccaggt ggcaaatcac gaagcagagt
    14401 ggaaagctgt actggatcac ttcttctcgg atttcaccca gcagttagat aaagctgaaa
    14461 aagatccgga agagggtggt atgcgcccga accagatggt tctgaccagc attgactgcc
    14521 cgacttgtgg tcgcaaaatg gggattcgca cagcgagcac cggggtattc cttggctgtt
    14581 ctggctatgc gctgccgccg aaagagcgtt gcaaaaccac cattaacctg gtgccggaaa
    14641 acgaagtgct gaacgtgctg gaaggcgaag atgctgaaac caacgcgctg cgcgcaaaac
    14701 gtcgttgccc gaaatgcggc acggcgatgg acagctatct catcgatccg aaacgtaagt
    14761 tgcatgtctg tggtaataac ccaacctgcg acggttacga gatcgaagag ggcgaattcc
    14821 gcattaaagg ttatgacggc ccgatcgttg agtgtgaaaa atgtggctct gaaatgcacc
    14881 tgaaaatggg gcgtttcggt aaatacatgg cctgcaccaa cgaagagtgt aaaaacacac
    14941 gtaagatttt acgtaacggc gaagtggcac caccgaaaga agatccggtg ccattacctg
    15001 agctgccgtg cgaaaaatca gatgcttatt tcgtgctgcg tgacggtgct gccggtgtgt
    15061 tcctggctgc caacactttc ccgaaatcgc gtgaaacgcg tgcgccactg gtggaagagc
    15121 tttatcgctt ccgcgaccgt ctgccggaaa aactgcgtta tctggccgat gcgccacagc
    15181 aggatccgga aggtaataag accatggttc gctttagccg taaaaccaaa cagcaatatg
    15241 tctcttcgga aaaagacgga aaggcgactg gctggtcagc attttatgtt gatggcaaat
    15301 gggttgaagg aaaaaaataa cctttaattc tgtcaggttt ttataaacaa agggtcgcga
    15361 aagcggccct tttttattgc atattat
//
LOCUS       DBANUU010000101        15223 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000101 DBANUU010000000
VERSION     DBANUU010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.101"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            104..376
                     /locus_tag="ACIE68_003318"
     CDS             104..376
                     /locus_tag="ACIE68_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFX5909584.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKTRQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(377..1249)
                     /gene="rluF"
                     /locus_tag="ACIE68_003319"
     CDS             complement(377..1249)
                     /gene="rluF"
                     /locus_tag="ACIE68_003319"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFX5909585.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEVKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1597..2544
                     /locus_tag="ACIE68_003320"
     CDS             1597..2544
                     /locus_tag="ACIE68_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFX5909586.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELRIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2614..3420
                     /locus_tag="ACIE68_003321"
     CDS             2614..3420
                     /locus_tag="ACIE68_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFX5909587.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSKRASYITGVTTNIAGGKTRG"
     gene            3430..3837
                     /locus_tag="ACIE68_003322"
     CDS             3430..3837
                     /locus_tag="ACIE68_003322"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFX5909588.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            3837..4332
                     /locus_tag="ACIE68_003323"
                     /pseudo
     CDS             3837..4332
                     /locus_tag="ACIE68_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            4398..5195
                     /locus_tag="ACIE68_003324"
     CDS             4398..5195
                     /locus_tag="ACIE68_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFX5909589.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQPQATTSTALDQLDD"
     gene            5206..6030
                     /locus_tag="ACIE68_003325"
     CDS             5206..6030
                     /locus_tag="ACIE68_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFX5909590.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            6082..7311
                     /gene="sorE"
                     /locus_tag="ACIE68_003326"
     CDS             6082..7311
                     /gene="sorE"
                     /locus_tag="ACIE68_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFX5909591.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            7694..8383
                     /gene="pepE"
                     /locus_tag="ACIE68_003327"
     CDS             7694..8383
                     /gene="pepE"
                     /locus_tag="ACIE68_003327"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFX5909592.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8474..10105)
                     /locus_tag="ACIE68_003328"
     CDS             complement(8474..10105)
                     /locus_tag="ACIE68_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFX5909593.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10325..14008)
                     /gene="metH"
                     /locus_tag="ACIE68_003329"
     CDS             complement(10325..14008)
                     /gene="metH"
                     /locus_tag="ACIE68_003329"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFX5909594.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14208..15032
                     /gene="iclR"
                     /locus_tag="ACIE68_003330"
     CDS             14208..15032
                     /gene="iclR"
                     /locus_tag="ACIE68_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFX5909595.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFATISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3633 a         4010 c         3980 g         3600 t
ORIGIN      
        1 caagaaagat aaatatttaa gaagagaaca aaggaatatt atcagttcag ctcgcaaacg
       61 aattcccgta cactgaactc tctggctcac tgagggcacc atcatggcac tcccccgtat
      121 tacccaaaaa gagatgactg aacgcgagca gcgtgaactt aaaacactgc tggatcgggc
      181 gcggattgcg catggtcgcg tactgaccaa ctccgaaacc aacagcatta agaaagagta
      241 catcgataaa ttaatggtcg agcgtgaggc tgaagcgaaa aaaacccgcc agttgaagaa
      301 aaagcaggct tataaaccgg atcctgaagc gtcatttagc tggtcagcaa atacgtcgac
      361 gcgcggcagg cgctgattag cgtcccttct ttttacgccc cggcgaggta aaacgcttac
      421 cgttggacgc cgggcgaccg cctttttccg ccgttttttc catcttaacg actggacgtt
      481 tgatgcccgc tgttttcggt ttagccttcg ctttaggttt tacctcggaa gaggaatttt
      541 caatgagttt aaacaggtcg attaactcat cgtcagttaa atcgcgccac tcacccagcg
      601 gaatgccgct taaactgacg ttcataatgc gcgtacgttc cagctttttc acttcatagc
      661 cgaaatgctc gcacatgcgc cggatctgac ggttcagccc ctgcaccagg gtaatgcgga
      721 agacaaacgg cgcttctttt ttgactttgc actttttcgt caccgtcccg aggatcggca
      781 cccccgcccc cataccacga ataaattcat cggtaatcgg tttatcgacc gtcaccagat
      841 actctttctc atgatcgtta cccgcacgga ggatcttatt caccagatcg ccgtgattgg
      901 tgaggaaaat cagcccctgg gagtctttat ccaggcggcc aatcgggaac acgcgtttgc
      961 tgtggttaac gaaatcgaca atgttatcgc gctcgccatc ttcggtggtg ctgacaatac
     1021 cgacgggctt gttcagggcg ataagtacca aatcttcggc ttcccgaggc tcaatcaact
     1081 gaccgtttac tttcacaatg tcgccgggtt tcacctgatc gccaatggtg gctcgcttgc
     1141 cattaaggaa cacattgcct tgctcgatat agcgatccgc ttcgcggcgt gagcaaattc
     1201 cgctttcgct gatgtattta tttaaacgga ctgatgagtc gggcagcata aattctcctg
     1261 taaaacgcgg aatataccgt acctcttagc ggggaaaaac agggttatgc ggcaacgtcg
     1321 aaattaaatg tgatctgcca cgccttttat cacaaaagtg tctccggaag tacgatgcaa
     1381 atttgcctgg caaaataagg ataatcaaca tattacgaat cgctgcacac ctgtgctgat
     1441 gactactacg cagagaaaca aatgtgcaag agaaagcgct atttctcccc atgcaaagag
     1501 tgaaaaatcg ctttgaaagg ccctcatgat ggggttatat cagatcaagc ggtgagaaag
     1561 cgtatttgca caaatgtgca gaaccaggag gcggcaatgg aaaacagtga cgatatccgt
     1621 ttgattgtga agattgccca actctattac gaacaggata tgacgcaggc gcaaatcgcg
     1681 cgcgaactgc gtatttaccg caccaccatc agccgcttgc ttaaacgagg ccgcgatcag
     1741 ggaattgtca ccatcgccat caactatgac tacaacgaaa atctctggct ggagcagcaa
     1801 ctgaagcaaa agtttggcct gaaagacgtt gtggtggtgt cgggaaatga tgaggatgaa
     1861 gagactcaac tggcgatgat ggggttacac ggcgcgcaac tgctggatcg cttgctggag
     1921 cctggcgata ttgtcggttt ttcctggggt cgcgcggtga gcgcactggt tgaaaacttg
     1981 ccgcaggcgg ggcaatcgcg gcagttaatc tgcgtaccaa ttattggcgg accgtccggt
     2041 aaactcgaaa gccgctatca tgtaaacaca ttaacctaca gcgcggcagc gaagctgaaa
     2101 ggggaatcgc atctcgcgga ttttccggct ctgctggata acccattaat tcgtaatggg
     2161 atcatgcagt ctcagcactt taaaaccatc tctgcctact gggataatct ggatgtcgcc
     2221 ctggtgggaa ttggctcacc ggccattcgc gacggcgcta actggcatgc gttttatggt
     2281 ggtgaagaga gtgacgacct gaatgcccgc caggttgctg gcgatatttg ctcgcgcttt
     2341 tttgatattc acggcgcaat ggttgaaacg aatatgagcg aaaaaacgct ctctatcgaa
     2401 atgaataaat taaagcaggc gcgatattcc attggcattg ccatgagtga agaaaaatac
     2461 agcggaatta ttggtgcact gcgtggaaaa tatattaatt gtctggtaac gaatagcagc
     2521 acagctgaac tattactgaa ataaaaatct tcccgatgtt taccgtaata aaaattacgg
     2581 cgggtcccta ttatctaaaa tcaggagtct gttatgcaaa cgtggttaaa tttgcaggat
     2641 aaaatcatta tcgtcaccgg cggcgcatcc ggtattggtc tggcgattgt agaggaatta
     2701 ttagcacaag gcgcaaacgt acagatggta gatattcacg gtggcgatgg tcaatatgaa
     2761 ggtcataaag gctatcagtt ttggccgacc gatatttcca gcgccaaaga ggtaaatcat
     2821 acggtagcgg aaattatcca gcgttttggt cgcatcgacg gtctggtcaa taacgccggg
     2881 gtcaatttcc cgcgtctgct ggtcgatgag aaagcgcctg ccgggcagta tgaactcaac
     2941 gaagctgcat tcgaaaaaat ggtcaatatc aaccagaaag gcgtttttct gatgtcgcag
     3001 gcggtggcgc gacagatggt caaacaacat gatggcgtga ttgtgaatgt ttcctcagaa
     3061 agtggactgg aaggctcaga aggccaaagc tgttacgccg ctaccaaagc cgcgcttaat
     3121 agcttcacgc gctcctggtc gaaagagctg ggtaaacacg gtatccgtgt ggtcggtatc
     3181 gcgcccggga ttctggaaaa aaccgggcta cgcacgccgg aatatgaaga ggcgctggcg
     3241 tggacgcgca atatcaccgt cgagcagctg cgtgaaggct ataccaaaaa cgccattcct
     3301 attgggcgcg ccggaagatt agcagaagtc gctgattttg tttgttatct gttgtctaaa
     3361 cgcgccagct acatcaccgg agtaaccact aacattgcgg gcggcaaaac gcgcggctaa
     3421 ggaggcttta tggtcaacgc cattttttgt gcccacggca aattagcttg cgccatgctg
     3481 gaatcggttc agatggttta cggcaacgcc aatgttgaag ccgttgaatt tgtgcccggc
     3541 gagaacgccg gagacatcgt agcaaagctg gaaaagttag taagcattca caatcaagat
     3601 gagtggctga ttgccgtgga tttacagtgc ggcagcccgt ggaatgcggc agcgatgctt
     3661 gcgatgcgca atcctcgtct gcgggtaatc agcggccttt ctctgccgct ggcgctcgaa
     3721 ctggttgata accaggacag catgaatgtt gatgaactgt gtgaacacct gacgcaaatc
     3781 gcaaagcaaa cctgcgttgt ctggaaacaa ctggcaacgg cagaggagga tttctgatga
     3841 acattacgct cgcccgcatt gatgaccgcc tgatccacgg tcaggtcact accgtctggt
     3901 cgaaagtggc taacgcccag cgcattatta tctgcaatga cgaagtttat aacgatgaag
     3961 ttcgccggac attattgcgt caggctgcgc caccgggaat gaaggttaac gtggtcaata
     4021 ttgaaaaagc cgtcgctgtt tatcacaacc cgcaatatca ggacgaaacc gttttttatc
     4081 ttttcactcg cccacaggat gccttagcga tggttcgcca gggggtaaaa atagacaccc
     4141 tgaatattgg cggcatggcc tggcggcccg gtaaaaaaac agttaaccaa agcggtttca
     4201 ttagatgatg atgatattaa cgcatttcat gagttaaata atcttggcgt catactggat
     4261 ttacgcgtcg tagcgtcaga tccctcaatc aatattatcg acaaaataaa cgaacagtta
     4321 atcgcaaatt aaataatagc gcctgtgtac atatgcttta acaggcaggg atgacgacac
     4381 ttaaaaggtg aaaaatcatg gaaataagta ccctacaaat cattgctata tttctttttt
     4441 cctgtattgc cggaatgggc agcgtgctgg atgaatttca gactcatcgc ccgttaattg
     4501 cctgtacggt gattggttta attctcggtg atttaaaaac cggaataata ctcggtggta
     4561 cgctggaatt gatagctctc ggctggatga acgtcggcgc ggcgcaatct ccggattctg
     4621 cactcgccag cataatctcc gccattctgg ttatcgttgg tcagcaaagc atcgccaccg
     4681 gaatcgccat cgcgttgcct gtggctgcgg caggccaggt gctgactgtg tttgcccgta
     4741 ccatcaccgt agtgttccag cacgcggcgg ataaagcagc ggaagaagca cggtttcgca
     4801 ctctcgatat tctgcatgtc tccgcgcttg gcgtgcaggc gctgcgcgtt gctattccgg
     4861 cactgattgt ctcactgttc gtcagcgccg atatggtgag caatatgctg agcgcgatac
     4921 cggaattcgt gacgcgcggt ctgcaaattg ctggcggttt tatcgtggtg gtcggttacg
     4981 ccatggtgct acgtatgatg ggcgtgaaat atttgatgcc tttctttttc ctcggtttcc
     5041 tcgcaggtgg ctacctcgat ctcagtctgc tggcgttcgg tggcgtcggc gtgatcatgg
     5101 ccctggtcta catccagcta aatccacagt ggcgtaaagc tgaacctcaa ccccaggcca
     5161 ccacctccac cgcccttgac caacttgacg attaacggag ccatcatgga acagagaaaa
     5221 attacacgca gcgatctggt gagcatgttt ctgcgctcca acctgcaaca ggcgtccttt
     5281 aactttgaac gtattcacgg gctgggcttt tgctacgaca tgatccccgc catcaagcga
     5341 ctttacccat taaaagagga tcaggttgcg gcgctcaggc gacacctggt gttcttcaat
     5401 accacgccag ccgtatgtgg cccggtcatc ggcgtcactg ccgccatgga agaggcgcgg
     5461 gccaacggcg cggaaattga tgacggtacc attaacggca tcaaagtcgg tctgatggga
     5521 ccattggcag gggttggcga tccactggtt tggggaacgc tgcgcccgat taccgctgcg
     5581 ctcggcgcat ctctggcact ttctggcaac atcctcggcc cgcttttgtt tttctttatt
     5641 ttcaatgcgg tgcgtctggc gatgaagtgg tatggcctac agctcggctt tcgcaaaggg
     5701 gtgaatatcg tcagcgatat gggcgggaat gtgctgcaaa aactcaccga aggcgcgtcg
     5761 attctcggac tgtttgtgat gggcgtgctg gtcaccaaat ggacgtcaat caacgtaccg
     5821 ttggtggttt cacaaacgca tgccgccgat ggctccaccg tcaccatgac cgtgcagaac
     5881 attctcgacc aactttgccc tggtttgctg gcgctcggtc tgacgctgct tatggttcgt
     5941 ctgctcaaca aaaaaattaa cccggtatgg ctgattttcg ccctgtttgg cttagggatt
     6001 atcggtaatg cgctgggctt cctgtcctga ttcttcgccc cggcacgact gccggggcca
     6061 tcgctcaaca tgaggtggtt tatgaaaaca acagctctgc gtctttatgg taaacgtgat
     6121 ttacgcctgg aaacctttga ccttcctgaa atgcaggagg atgaaatcct cgcgacggtg
     6181 gtcactgaca gcctgtgcct ctcttcctgg aaagaggcca atctgggtga aaaccataaa
     6241 aaagtacccg acgatgtggc gaccaacccc atcatcatcg gccacgagtt ttgcggcgat
     6301 attctggccg tgggtaaaaa gtggcagcac aaattccagc cgggtcagcg ttatgtgatt
     6361 caggccaacc tgcaactccc cgaccgcccg gactgccccg gctactcctt cccgtgggtg
     6421 ggcggcgaag ccacgcatgt ggttattccc aacgaggtca tggaacaaga ttgcctgctg
     6481 gcatacgacg gcgaaaccta ttttgaaggc tcactggttg aaccgctttc ctgcgtgatt
     6541 ggcgcgttca acgccaacta tcatcttcag gaaggtagtt ataaccacac gatggggatt
     6601 cgcccgcaag ggcgcatgct gatcctcggc ggcaccggac caatgggact gttggcgatt
     6661 gattatgcgc tacatggacc cgttaacccg tcgctgctcg tcattaccga taccgacaac
     6721 gataaattga gttatgcgcg caagcactat ccatcagaac cgcaaacact gattcattat
     6781 ctcaatgccg ccgatgcagc atttgatacg ctaatggcgc tgagtggcgg tcacggcttc
     6841 gatgatattt tcgtctttgt gcctaatgaa ggactggtga ctctcgcctc ttccttgctg
     6901 gcgacagatg gttgcctgaa tttcttcgcc ggaccgcagg ataaacattt cagcgcgcca
     6961 attaatttct acgatgtgca ttatgcattt acccactacg tgggcacgtc aggcggcaat
     7021 accgacgaca tgcgcgcggc ggtcaaactg attgaagaga aaaaagtgca ggccgcaaaa
     7081 gtggtaacac atattcttgg gctgaatgcc gcgggcgaaa ccacgcttga attgcctgcc
     7141 gtcggcggcg gcaaaaagct ggtgtatacc gggaaatacc tgccgctgac gtcactcacg
     7201 cagattcagg atcaagcact ggcggcgatt ctggcgcgtc atcaggggat ctggtccggt
     7261 gaggcggagc aatatctgct cactcatgca gaggcaattt cccatgatta atcgcgctac
     7321 ttaactctgc atgtcgctgg ctggtcgccg cggcaacttc gcgacctgat gtgcggctga
     7381 ttgctgaagc ggcccaatgt gcacgagaag gataaaaaaa catcagcgcc ttaaggggcg
     7441 ctcaaattgg tggcaaagta cacattgttt atgccggatg cggaataaag ccttattcgg
     7501 cctacataat ctcgaaaatt caatatattg cagttatctg gtaggcctgg taagcgtagc
     7561 gcatcaggca atgctgtgtt tgtcatcagt ctcagcgccc ggtgcaatac gtgacatcgt
     7621 gagccatgtc agtaagtggt taaattcgca gtggtagcat gcctcccgtg atttcttact
     7681 aactggagaa gtaatggaac tgcttttatt gagtaactcg acgctgccgg gtaaagcctg
     7741 gctggaacat gcgctgccgc tgattgctga acagttgcag ggtcgccgtt cagcggtatt
     7801 tattcctttc gctggcgtaa cgcagacctg ggatgattac acagcgaaaa cggctgcggt
     7861 tctcgctccg ctgggtgtat ctgtaaccgg tattcatagc gttgtcgatc ccgttgccgc
     7921 gattgaaaat gctgagatcg tgattgtcgg gggcgggaat actttccagt tgctgaaaca
     7981 gtgccgcgag cgcgggctgc tggcaccaat tactgacgtg gttaaacgtg gtgcgctgta
     8041 tattggctgg agcgcaggcg ctaaccttgc ttgcccaacc attcgtacca ccaacgatat
     8101 gccgattgtc gatccgcaag gtttcgatgc gctaaatctg ttcccgctgc aaatcaaccc
     8161 gcacttcacc aacgcgctgc cggaaggcca taaaggtgaa acccgtgagc agcgtattcg
     8221 cgaactgctg gtcgtcgcgc cagaactgac gattattggt ctaccggaag gtaactggat
     8281 cacagtgagt aaaggtcacg ctacgctggg tggcccgaac accacttatg tgtttaaggc
     8341 tggtgaagaa gcggtgccgc tagaagctgg tcaccgtttt taagtaaatt gccggatgca
     8401 tgatccggca tcttgttcat agtgcctgat gcgacgcttg acgcgtctta tcaggcctac
     8461 aggattcaca atattaatac tcgtcccgtc cttcatcttc atccggctgt tccagcacac
     8521 tgtaagccac cgaacaaaac agcgagttca ggcgctgcat atctcccagt aagcctaaat
     8581 gtagcgaact ggtttcaatg ctctgcacgt tttgctgatg caggcgatcg acgtgggcgt
     8641 gggaatagcg gcgattaaga atgcgaaaac gatgtttgct gcgacgcaaa cgacgagcgc
     8701 tggtgacatc gccagagaag aacaccgaca ttgccagctt taaattactg agcaattgct
     8761 catagagcgc atccagttcc ttcaacccat caagcgaaaa tgcccgccgt gctgccagcg
     8821 atttatcggc aatttcgctg cccatgcgct cgacgatatc ggaagcctgt tcaaggttga
     8881 gcgacatttc aataatctcc gcccagcgac gcgactcttc ttctgccagt tcctcttttg
     8941 gcatccgcgc cagatacagc ttaatggcgg tatagagaac gttgatatca tctgccagct
     9001 tacgcagctc tttctcctgc cgtggctcgc cgtgcatcac tttattcagc ccttccatca
     9061 tctgttccat ggcgtcgcca atgcgcaggg tttcgcgcgc ggcgttcgcc agagcaagcg
     9121 tgggcgtatc cagcgcgctg acatccagat gtttaggccg tagctgggta tccagttccg
     9181 gttcatcgcg aataatcgtt ttgcaaaacc gtgccatcgg gtcaacaaat ggcagcatga
     9241 ccaggcaacg tacaaggttg tagaagacgt ggaaatagat caccagttcc gcttttggca
     9301 atgacagctt ccccattgtc tctgccagca aatggacaaa cggcaggata atcaggctac
     9361 ccaccagctt aaacagcaga ctacccagcg cgacacggcg ggctgcggca ttggcggcac
     9421 tgttgttgag catcgccagc aggccggaac cgaggttagc accaatcacc agacagagcg
     9481 ccacggggaa ggagataatg cctgcggcgg tcagagtcgc agtcagcagt acagcagcaa
     9541 ggctggagta gctgataatg gcgaacatcg cgccaatcag cgcatccagc agaatatcgc
     9601 cggtcagcga ggcaaagatc acctgaacgc cgtttgcctg ggtgatcggc gttacggcct
     9661 gcacaatcaa ctccagcgcc agtaaaatca gcccaagacc aataccgacg cggcccagtt
     9721 gcccggcgcg tgactgtttg cgtccgagga aaaaaatcac gccgataaaa ataagtaacg
     9781 gtgacagcca ggataagtcg aaggtgagaa tacgcgccat tagcgccgtc ccgacatctg
     9841 cccccagcac aatgaccaga gccggtgcga gggctaccag atcctgagcg acaaacgagg
     9901 tcaccagcat ggtggtggca ttactgctct gcaccagtgc ggtaacgccg atccccgcgc
     9961 aaaaggcgag cggctttttt tcgacgctcc ggctaaggac agtacgcaaa cgcgcgccga
    10021 agacgcgcat tacgccggtt cgaacaatat gagtccccca gaccagcagg gcgacggcag
    10081 aaagcaggtg aagcagcgtt aacacgagtt caggttctcc ttatcgttat atgttcctga
    10141 agcaattgaa tgaccaaagg accggaaaca cgctgactgg catctttact gtgccttttg
    10201 acattcgttt ttccggctaa gattattcca gcctgtttcc agtataagtg tttcatatta
    10261 ataaagagac agggcactca ttgagcgccc tgtttgttgt aaggaaaagt gacagatttg
    10321 tgaatcagtc cgcgtcatac cccagattcg gtgccagcca gcgctcaaca tcggagacgc
    10381 tcataccttt acggcgggca tagtcttcaa cctgatcgcg ctgaatttgc gccaccgcgt
    10441 agtacttgct gtccgggtgg ctgaaatacc aacccgaaac cgatgcaccg ggccacatgg
    10501 cgaaagattc tgtgagtttc atgccggtat gtttttccac ttccagcagc tcccagatgg
    10561 tggctttttc cgtatgttcc gggcaggccg ggtagcccgg tgccggacgg atgccctggt
    10621 agttttcgcg gatcagttct tcgttgctga ggttctcgtt cggcgcatag ccccagtaga
    10681 ctttacgcac acgctcatgg agatactccg caaaggcttc ggctaaacgg tcggcaagcg
    10741 ctttcaccat gattttgttg taatcatcgt gctgcgcttc aaaggcatca gccagtgcgt
    10801 cctcttccag cccgccagtc acggcaaatg cgccgatgta atctgcttta ccagaaagct
    10861 tcggcgcaac gaagtcagcg agacagtagt tagcgaatcc cgttttttcg gtctgttgac
    10921 gcagatggtg gctgacgttg atcacatggg tgcgcgtttc gtcacggtag atttcaatgt
    10981 catcgcccac acggtttgcc gggaacaggc ccaccacgcc acgcggattc agcgttttct
    11041 cggcgcttaa tttatccagc atgtcgttgg cgtctttaaa cagccgctgc gcctcaacgc
    11101 ccaccacttc atcttccaga atgcgcggat acttcccggc cagcgaccag gtcataaaga
    11161 acggtgtcca gtcgatgtaa ttacgcagag tttcgatgct ggcttcaact tcctgcacgc
    11221 cgagacggtg cgccaccggc ggcgtgtaag cctgccagtc aaaagcgaaa tcgttatcgc
    11281 gcgccgcttc cagcgtgacc ggtggtgtgc gcggtttctt acgcccgtgc tgaatacgta
    11341 cggtttcgta ctccttgcgg gtacgagcga caaaatcatc acgctgagta tcggaaagca
    11401 gcgccgccac cacaccaacg gtgcgcgatg cgttctgtac atacaccgtc gggccgctgt
    11461 agttctgctc aattttcacc gccgtgtgcg cttttgaggt cgtcgcgccg ccaatcaata
    11521 gcgggatggt gaagccctga cgctccatct ctttcgccac gttaaccatc tcgtccagcg
    11581 acggcgtgat aagccccgaa agtccaatca gatcagcatt cacttcttta gcggtacgga
    11641 gaattttttc cgcaggcacc ataacgccga gatcgacaat ttcgtagttg ttacattgca
    11701 gcaccacacc aacgatattt ttaccgatgt cgtggacgtc gcccttcacg gtggcgatca
    11761 ccatcttgcc gttggttttg ccctgctctt tgctggcttc aataaacggt tcgagatacg
    11821 ccaccgcctg tttcatgacg cgcgccgatt tgaccacctg tggcaggaac attttccctt
    11881 cgccaaacag atcgccgacc acgttcatgc cgtccatcaa cgggccttca atcacttcaa
    11941 tcgggcgcgt agcctgctgg cgggcttctt cggtatcctg ctcgataaac tcggtaatgc
    12001 ctttgaccag cgagtattcc agacgtttat tcacttccca cgagcgccac tccgcctgct
    12061 gggcgttagc ggtgtcgtcg gttttgctgc cgcgatattt ctcggcaagc tccagtaaac
    12121 gctcagtgcc atcatcgcga cgattaagaa tcacatcttc caccgcgtcg cgcagttcag
    12181 cgggcaggtc gtcgtaaatc gccagttgcc cggcattgac gatccccata tccatgccat
    12241 tgcgaatagc gtagtagagg aacactgcgt gaatggcttc gcgcaccgga tcgttgccac
    12301 ggaacgagaa agaaacgtta gatacgccgc cggaaattag cgcgtgcggc agttcgcgtt
    12361 tgatgtcttc acacgcgccg ataaagtcct gcgcgtagtt gttgtgctct tcaatgccgg
    12421 tggcaaccgc gaagatgttc gggtcgaaga tgatatcttc tggcgggaag ccaacctctt
    12481 cggtgaggat tttgtacgcc cgacggcaaa tctcgatttt ccgtgcgcgg gtatcggcct
    12541 gtccctgttc gtcaaaggcc attaccacca ctgccgcacc gtaacgacgc aagagcttcg
    12601 cgtgatggat aaaggcgtcg acgccctctt tcatcgagat agagttaaca atgcctttgc
    12661 cctggataca cttcagacct ttttcaatga catcccattt tgaggagtcg atcataatcg
    12721 gcacgcgagc gatatccggt tcaccggcaa tcagattgag aaaacgcacc atcgccgctt
    12781 cggcatcgag catcccttca tccatgttga tatcgataat ctgcgcaccg ttttccacct
    12841 gctggcgggc cacatccagc gcctcgctgt atttctcttc tttgatcagg cgcttgaact
    12901 tagcggaacc ggtgacgtta gtccgctcgc cgacgttcac aaacaggctg tcgtcgccaa
    12961 tgttcagcgg ctccaggccg gacaaacggc aggctacggg aatttcaggc agtttgcgcg
    13021 gcgctaatcc ttctactgca cgactcatcg ctgcaatatg ttgtggcgtg gtgccacagc
    13081 agccgccgac gatattgaga aaacccgctt gcgcccattc acgtatctgt tttgccatcg
    13141 tgtcggcgtc gagatcgtac tcaccaaagg cgttgggtag cccggcgttc gggtgcgcgg
    13201 tgacgtagca ttccgcaatc cgtgacagct cctgcacgta ctggcgcagt tcatctggcc
    13261 ccagcgcaca gttcaggcca aaggtcagag cttcggcgtg gcgcaatgag ttgtaaaatg
    13321 cttcggtggt ctgcccggag agcgtgcgcc cggaggcgtc ggtgatggtg ccggagatca
    13381 taatcggcag ctcaacgccc agcgcttcaa actccgtttt caccgcaaag accgccgctt
    13441 tggcgttaag ggtgtcgaaa acggtttcaa tcaggatcag atccgcacca ccttccacca
    13501 gcgctttggt ggactctcga taagccgcca ccagcccgtc aaaagtgata ttacgaaatg
    13561 ccggatcgtt gacgtccgga gaaatagacg ccgtgcggtt ggttgggccg agaacaccgg
    13621 caacgtagcg cggtttctcc ggggtgcgcg cggtccactc atcagcacag gcgcgcgcca
    13681 gtttcgccgc cgcaaagttg atttccgccg acagggattc catctggtaa tccgccatcg
    13741 caatggtcgt ggagttgaag gtgttggttt cgatgatatc cgcgcccgct tcaaagtagg
    13801 cgttgtggat agcggcgatc acttccggtt tactgagtac cagcaggtcg ttgttgcctt
    13861 tgaggtcgca tggccagtcg gcaaagcgtt caccacgaaa atcggcttcg ttcagtcgat
    13921 agctctggat catggtgccc ataccgccgt ccagcaccag aatacgttca tttaactgcg
    13981 cacgcagttg ttccactttg ctgctcacac ttgctcccga cacgctcaac ccagatttaa
    14041 taaaaattca acaaaccata ctggcataaa cgcatctgtg gtaaaagcga ccaccacgca
    14101 acatgagatt tgttcaacat taactcatcg gatcagttca gcaactattg cattagctaa
    14161 caataaaaat gaaaatgatt tccacgatac agaaaaagga gactgtcatg gtcgcaccca
    14221 ttcccgcgaa acgcggcaga aaacccgccg ttgccaccgc accagcgact ggacaggttc
    14281 agtctttaac gcgtggcctg aaattactgg agtggattgc cgaatccaat ggcagtgtgg
    14341 cactcacgga actggcgcaa caagccgggt tacccaattc cacgacccac cgcctgctaa
    14401 ccacgatgca acagcagggt ttcgtgcgtc aggttggcga actgggacac tgggcaatcg
    14461 gcgcacatgc ctttatggtc ggcagcagct ttctccagag ccgtaatttg ttagcaattg
    14521 ttcaccctat cctgcgcaat ctaatggaag agtctggcga aacggtcaat atggcggtgc
    14581 ttgatcaaag cgatcacgaa gcgattatta tcgaccaggt acagtgtacc cacctgatgc
    14641 gaatgtccgc gcctatcggc ggtaaattgc cgatgcacgc ttccggtgcg ggtaaagcct
    14701 ttttagccca actgagcgaa gaacaggtga cgaagctgct gcaccgcaaa gggttacatg
    14761 cctataccca cgcaacgctg gtgtctcctg tgcatttaaa agaagatctc gcccaaacgc
    14821 gcaaacgggg ttattcattt gacgatgagg aacatgcgct ggggctacgt tgccttgcag
    14881 cgtgtatttt cgatgagcac cgcgaaccgt ttgccacaat ttcaatttcc ggaccgattt
    14941 cacgtattac cgatgaccgc gtgaccgagt ttggcgcaat ggtgattaaa gcggcgaagg
    15001 aagtgacgct ggcgtacggt ggaatgcgct gacttttttc tggcgggcag aggcaatatt
    15061 ctgcccgtca tatctgagtg gcaatagaat aagggtgtct gttaatcgca ttgacgccaa
    15121 aataacttat tgtcatacac ttcgcacact tttattaact cagcattatt tttaaacatc
    15181 aaaccactta attattaaca aaatcgcaag atctcattac gcc
//
LOCUS       DBANUU010000102        15119 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000102 DBANUU010000000
VERSION     DBANUU010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.102"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            2..979
                     /gene="ybeQ"
                     /locus_tag="ACIE68_003331"
     CDS             2..979
                     /gene="ybeQ"
                     /locus_tag="ACIE68_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFX5909596.1"
                     /translation="MIFTSTCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(1049..1531)
                     /gene="ybeL"
                     /locus_tag="ACIE68_003332"
     CDS             complement(1049..1531)
                     /gene="ybeL"
                     /locus_tag="ACIE68_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFX5909597.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1766..4348
                     /gene="leuS"
                     /locus_tag="ACIE68_003333"
     CDS             1766..4348
                     /gene="leuS"
                     /locus_tag="ACIE68_003333"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFX5909598.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4363..4944
                     /gene="lptE"
                     /locus_tag="ACIE68_003334"
     CDS             4363..4944
                     /gene="lptE"
                     /locus_tag="ACIE68_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFX5909599.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4944..5975
                     /gene="holA"
                     /locus_tag="ACIE68_003335"
     CDS             4944..5975
                     /gene="holA"
                     /locus_tag="ACIE68_003335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFX5909600.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            5977..6618
                     /gene="nadD"
                     /locus_tag="ACIE68_003336"
     CDS             5977..6618
                     /gene="nadD"
                     /locus_tag="ACIE68_003336"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFX5909601.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6642..7253
                     /gene="cobC"
                     /locus_tag="ACIE68_003337"
     CDS             6642..7253
                     /gene="cobC"
                     /locus_tag="ACIE68_003337"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFX5909602.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSTIDINQKFATLRVLNSRAIGVENA"
     gene            7513..7830
                     /gene="rsfS"
                     /locus_tag="ACIE68_003338"
     CDS             7513..7830
                     /gene="rsfS"
                     /locus_tag="ACIE68_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFX5909603.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7834..8301
                     /gene="rlmH"
                     /locus_tag="ACIE68_003339"
     CDS             7834..8301
                     /gene="rlmH"
                     /locus_tag="ACIE68_003339"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFX5909604.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8332..10233
                     /gene="mrdA"
                     /locus_tag="ACIE68_003340"
     CDS             8332..10233
                     /gene="mrdA"
                     /locus_tag="ACIE68_003340"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFX5909605.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10236..11348
                     /gene="mrdB"
                     /locus_tag="ACIE68_003341"
                     /gene_synonym="rodA"
     CDS             10236..11348
                     /gene="mrdB"
                     /locus_tag="ACIE68_003341"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFX5909606.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11359..12447
                     /gene="rlpA"
                     /locus_tag="ACIE68_003342"
     CDS             11359..12447
                     /gene="rlpA"
                     /locus_tag="ACIE68_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFX5909607.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12587..13798
                     /gene="dacA"
                     /locus_tag="ACIE68_003343"
     CDS             12587..13798
                     /gene="dacA"
                     /locus_tag="ACIE68_003343"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFX5909608.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13909..14172
                     /gene="ybeD"
                     /locus_tag="ACIE68_003344"
     CDS             13909..14172
                     /gene="ybeD"
                     /locus_tag="ACIE68_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFX5909609.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14273..14914
                     /gene="lipB"
                     /locus_tag="ACIE68_003345"
     CDS             14273..14914
                     /gene="lipB"
                     /locus_tag="ACIE68_003345"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFX5909610.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         3726 a         3837 c         3955 g         3601 t
ORIGIN      
        1 aatgattttc acgtcaactt gctgcgataa tttatcaata gatgaaatta tcgaacgtgc
       61 tgaaaaaggc gattgcgagg ctcaatatat tgttgggttt tattataatc gcgatagcgc
      121 aattgattct ccggacgacg aaaaagcctt ttactggctg aagctggccg ccgagcaagg
      181 tcattgtgaa gcacaatatt ccttaggaca aaagtatact gaggataaaa gccgtcataa
      241 agataatgag catgcaatct tttggctgaa aaaagctgcc ctacaaggcc atactttcgc
      301 ttccaacgcc cttggctgga tactggatcg tggagaagac cccaattata aagaagcggt
      361 tgtctggtat cagatagccg cggagagcgg aatgtcttat gcgcaaaata atcttgggtg
      421 gatgtacaga aatggcaacg gagtcgcaaa agactatgcg ctggcatttt tctggtacaa
      481 acaagctgca ttacaaggcc atagtgacgc gcaaaacaat ctggccgatc tttatgaaga
      541 cggaaaaggc gttgctcaaa acgagacact cgccgcattc tggtatttga aaagcgcaca
      601 gcagggtaat cggcatgctc agtttcaaat tgcatgggat tataacgctg gcgaaggggt
      661 ggaccaggac tataagcaag cgatgtactg gtatctgaag gctgccgctc aggggagcgt
      721 cggcgcttac gtcaacatcg gttatatgta taaacacgga caaggcgttg agaaagatta
      781 tcaggctgcc tttgaatggt ttacgaaagc cgctgaatgc aatgacgcca ctgcctggta
      841 taacctggcc attatgtatc actacggaga aggaagacct gtcgatctcc gacaggctct
      901 cgacctgtat cgtaaagttc agtcatccgg aaccagagat gtcagtcagg aaattcgtga
      961 gactgaagat ttactgtaga gataaaaaca agggccgggg aatttcattc ggcccatatt
     1021 ctctttattg ctgtgcgaaa tcagactatt acggctcaaa cgggcgtctc tggaactggt
     1081 catgaccaca tttcgggcat agcgtcagca cttccggtgt gtagatcggg agatggaagt
     1141 gacatttctc gcagaccaga tttcccagcc cgaccacttc tccgctgtga taaaccccat
     1201 gatgattgag gtcctggaaa acttcgcgcc attcaagctg cgttttatcg gtgatatctg
     1261 ccagctcctg ccataagctt tctttaatca cccgcataaa gacgctgtca gattcttctt
     1321 tcaggctctc ttcatagctc atggcgaact cttccaggtc acgtcttaca gctcgcgtca
     1381 gctcatcgac ctcggttcgc gttaactccc ctgtttttat tacgcgctcg cgcgcctgtt
     1441 ccaccagtgc gtcgatatca cgttcgccat tgcgcaggcg ttcgctcagt gacgcgacca
     1501 gttcacggta atattgagca accttgttca tcgtttcatc tcccgggtcg ttaactgact
     1561 ataataatag acgttctttg ttccttgctt tgctaatacg cccacaaact ctgtaaatta
     1621 atgtatgaat aatccgggca tcatttcaac gtcgagtgcg cgaaaggctg ttttgacgcg
     1681 gacgtttggg ctatgctatg cggatctgaa aaaccacctc aacgctacat ttgtagccgt
     1741 attgaaaaca ggaccactgg ctgccatgca agagcaatac cgcccggaag agatagaatc
     1801 caaagtacag cttcattggg atgagaagcg cacatttgaa gtaaccgaag acgagagcaa
     1861 agagaagtat tactgcctgt ctatgcttcc ctatccttct ggtcgactac acatgggcca
     1921 cgtacgtaac tacaccatcg gtgacgtgat cgcccgctac cagcgtatgc tgggcaaaaa
     1981 cgtcctgcag ccgatcggct gggacgcgtt tggtctgcct gcggaaggcg cggcggtgaa
     2041 aaacaacacc gctccggcac cgtggacgta cgacaacatc gcgtatatga aaaaccagct
     2101 caaaatgctg ggctttggtt atgactggag ccgcgagctg gcaacctgta cgccggaata
     2161 ctaccgttgg gaacagaaat tcttcaccga gctgtataaa aaaggcctgg tatataagaa
     2221 gacttctgcg gtcaactggt gcccgaacga ccagaccgta ctggcgaacg aacaagttat
     2281 cgacggctgc tgctggcgct gcgataccaa agttgaacgt aaagagatcc cgcagtggtt
     2341 tatcaaaatc actgcttacg ctgacgagct gctcaacgat ctggataaac tggatcactg
     2401 gccagacacc gttaaaacca tgcagcgtaa ctggatcggt cgttccgaag gcgtggagat
     2461 caccttcaac gttaacgact atgacaacac gctgaccgtt tacactaccc gcccggacac
     2521 ctttatgggt tgtacctacc tggcggtagc tgcgggtcat ccgctggcgc agaaagcggc
     2581 ggaaaataat cctgaactgg cggcctttat tgacgaatgc cgtaacacca aagttgccga
     2641 agctgaaatg gcgacgatgg agaaaaaagg cgtcgatact ggctttaaag ctgttcaccc
     2701 attaacgggc gaagaaattc ccgtttgggc agcaaacttc gtattgatgg agtacggcac
     2761 gggcgcagtt atggcggttc cgggtcacga ccagcgcgac tacgagtttg cctctaaata
     2821 cggcctgaac atcaaaccag ttatcctggc tgctgacggc tctgagccag atctttctca
     2881 gcaagccctg actgaaaaag gcgtgctgtt caactctggc gaattcaacg gtcttgacca
     2941 tgaagcggcc ttcaacgcca tcgccgataa actgactgcg atgggcgttg gcgagcgtaa
     3001 agtgaactac cgcctgcgcg actggggtgt ttctcgtcag cgttactggg gcgcgccgat
     3061 tccgatggtg acgctggaag acggtaccgt aatgccgacc ccggacgacc agctgccggt
     3121 gatcctgccg gaagatgtgg tgatggacgg cattaccagc ccgattaaag cagatccgga
     3181 gtgggcaaaa actaccgtta acggtatgcc agcgctgcgt gaaaccgaca ctttcgacac
     3241 ctttatggag tcctcctggt actatgcgcg ctacacttgc ccagagtaca aagaaggtat
     3301 gctggattcc gaagcggcta actactggct gccggtggat atctacattg gtggtatcga
     3361 acacgccatt atgcacctgc tctacttccg cttcttccac aaactgatgc gtgatgcagg
     3421 catggtgaac tctgacgaac cagcgaaaca gttgctgtgt cagggtatgg tgctggcaga
     3481 tgccttctac tatgttggcg aaaacggcga acgtaactgg gtttccccgg ttgatgctat
     3541 cgttgaacgt gacgagaaag gccgtatcgt gaaagcgaaa gatgcggcag gccatgaact
     3601 ggtttatacc ggcatgagca aaatgtccaa gtcgaagaac aacggtatcg acccgcaggt
     3661 gatggttgaa cgttacggcg cggacaccgt tcgtctgttt atgatgtttg cttctccggc
     3721 tgatatgact ctcgaatggc aggaatccgg tgtggaaggg gctaaccgct tcctgaaacg
     3781 tgtctggaaa ctggtttacg agcacacagc aaaaggtgat gttgcggcac tgaacgttga
     3841 tgcgctgact gaagatcaga aagcgctgcg tcgcgatgtg cataaaacga tcgctaaagt
     3901 gaccgatgat atcggccgtc gtcagacctt caacaccgca attgcggcga ttatggagct
     3961 gatgaacaaa ctggcgaaag caccaaccga tggcgagcag gatcgcgctc tgatgcagga
     4021 agcgctgctg gccgttgtcc gtatgcttaa cccgttcacc ccgcacatct gcttcacgct
     4081 gtggcaggaa ctgaaaggcg aaggcgatat cgacaacgcg ccgtggccgg ttgctgacga
     4141 aaaagcgatg gtggaagact ccacgctggt cgtggtgcag gttaacggta aagtccgtgc
     4201 caaaatcacc gttccggtgg acgcaacgga agaacaggtt cgcgaacgtg ctggccagga
     4261 acatctggta gcaaaatatc ttgatggcgt taccgtgcgt aaagtgattt acgtaccagg
     4321 taaactcctc aatctggtcg ttggctaagc gcgggaggaa gcgtgcgata tctggcaaca
     4381 ttgttgttat ctctggcggt gttaatcacc gccgggtgtg gctggcatct gcgtgatacc
     4441 acgcaggttc cttccactat gaaggtcatg atcctggact caggcgatcc gaacgggcca
     4501 ttaagccgtg cggtgcgtaa ccagttacgt ctgaatggtg tcgagttgct tgataaagaa
     4561 accacgcgta aggacgttcc atccctgcgt ttgggtaaag tgagcatcgc gaaagatacc
     4621 gcatcggtat tccgtaacgg tcaaacagca gagtatcaga tgatcatgac ggtgaatgcg
     4681 accgtgttga tccccggccg tgatatctac ccgattagcg ccaaagtctt ccgttcgttc
     4741 tttgataacc cgcaaatggc gttagcgaaa gataacgaac aagacatgat cgtaaaagag
     4801 atgtacgacc gtgctgccga acagctgatt cgtaagctgc caagcatccg tgctgcggat
     4861 attcgttccg acgaagaaca gacgtcgacc acaacggata ctccggcaac gcctgcacgc
     4921 gtctccacca cgctgggtaa ctgatgattc ggttgtaccc ggaacaactc cgcgcgcagc
     4981 tcaatgaagg gctgcgcgcg gcgtatcttt tacttggtaa cgatcctctg ttattgcagg
     5041 aaagccagga cgctgttcgt caggtagctg cggcacaagg attcgaagaa caccacactt
     5101 tttccattga tcccaacact gactggaatg cgatcttttc gttatgccag gctatgagtc
     5161 tgtttgccag tcgacaaacg ctattgctgt tgttaccaga aaacggaccg aatgcggcga
     5221 tcaatgaaca acttctcaca ctcaccggac ttctgcatga cgacctgctg ttgatcgtcc
     5281 gcggtaataa attaagcaaa gcgcaagaaa atgccgcctg gtttactgcg cttgcgaatc
     5341 gcagcgtgca ggtgacctgt cagacaccgg agcaggctca gcttccccgc tgggttgctg
     5401 cgcgcgcaaa acagctcaac ttagaactgg atgacgctgc aaatcaggtg ctctgctact
     5461 gttatgaagg taacctgctg gcgctggctc aggcactgga gcgtttatcg ctgctctggc
     5521 cagacggcaa attgacatta ccgcgcgttg aacaggcggt gaatgatgcc gcgcatttca
     5581 ccccttttca ttgggttgat gctttgttga tgggaaaaag taagcgcgcg ttgcatattc
     5641 ttcagcaact gcgtctggaa ggcagcgagc cggttatttt gttgcgcaca ttacaacgtg
     5701 aactgttgtt actggttaac ctgaaacgcc agtctgccca tacgccactg cgtgcgttgt
     5761 ttgataagca tcgggtatgg cagaaccgcc ggggcatgat gggcgaggcg ttaaatcgct
     5821 taagtcagcc gcagttacgt caggccgtgc aactcctgac acgaacggaa ctcaccctca
     5881 aacaagatta cggtcagtca gtgtgggcag agctggaagg gttatctctt ctgttgtgcc
     5941 ataaacccct ggcggacgta tttatcgacg gttgatatga aatctttaca ggctctgttt
     6001 ggcggcacct ttgatccggt gcactatggt catcttaaac ccgtggaaac gctggcgaat
     6061 ttgattggtc tgacgcgggt cacaatcatc cctaataatg ttcctccgca tcgtccccag
     6121 ccggaagcga acagcgtgca gcgtaaacac atgcttgaac tggcgattgc cgacaagcca
     6181 ttatttactc ttgatgaacg cgagctaaag cgcaatgccc cctcttacac tgcgcaaaca
     6241 ctgaaagagt ggcggcagga acaagggccg gacgtgccgc tggcgtttat tattggtcag
     6301 gattcattgc tgacctttcc gacctggtac gaatacgaaa cgatactcga caatgcacat
     6361 ttgatcgtct gtcggcgtcc aggttaccca cttgaaatgg cgcaaccgca ataccagcaa
     6421 tggctggaag atcatttgac acataacccg gaagatcttc accttcagcc tgccggtaaa
     6481 atttatctgg ctgaaacgcc gtggtttaac atctcggcga ccatcatccg cgaacgtttg
     6541 caaaacggtg aatcgtgtga ggatttattg ccggaaccgg tactgactta cattaaccaa
     6601 caaggcttgt atcgctgata cctgtcgtta cggagggcat gatgcgactg tggttaattc
     6661 gtcatggcga aacgcaagcg aatgtcgatg gtctgtacag cggtcatgcg cccacacccc
     6721 tgaccgcacg cggtattgag caagcgcaaa atctgcatac gctgctacat ggtgtttcct
     6781 ttgatctggt tttatgcagt gaactggaac gggcacagca taccgcgcga ctggttctca
     6841 gtgaccgcca gctccccgtg caaatcatcc ctgaactcaa cgaaatgttt tttggcgact
     6901 gggagatgcg acatcatcgc gacctcatgc aagaagatgc cgaaaactat agcgcgtggt
     6961 gcaatgactg gcagcatgca atccccacga acggtgaagg atttcaggca ttttcgcaac
     7021 gtgtggaacg ctttatcgca aggcttagtg aatttcagca ctatcagaat attttagtcg
     7081 tcagccatca gggtgtactg agtctgttaa tcgcccgttt aattggcatg cctgccgagg
     7141 ctatgtggca ttttcgcgtt gaccaggggt gttggagcac cattgatatc aaccaaaaat
     7201 tcgcgacgct acgcgtcctc aatagccgtg ccatcggggt cgagaatgca tgacttttct
     7261 gtttttttac gggtaagccg caacgaccat tgacagaccc gggcaggctg atattctccg
     7321 cagccagact tttttcgcca gacacgactt tgtagaaatt gttttacaaa aatggcgatg
     7381 caatctgcgg cgcggggtgg gatgatagcc cactttcgaa agccgattcg gcgacaattg
     7441 tcccgaaatc gcctctggtt caggtatact gacagaccat ttttatctat ttgattcacc
     7501 cagggggaaa acttgcaggg taaagcactc caggattttg ttatcgacaa aattgatgac
     7561 ctcaaaggtc aggacatcat cgccttagac gttcaaggca aatccagcat caccgactgc
     7621 atgatcatct gtacgggtac gtccagccgt catgttatgt ccattgctga ccacgttgtg
     7681 caggagtctc gcgcagcggg cctgttaccg ctcggcgtag aaggtgaaaa cagcgccgac
     7741 tggattgtcg tggatttggg cgatgtgatt gtccatgtca tgcaggaaga gagccgtcgc
     7801 ctgtatgaac tggaaaaact ctggagttaa tgcgtgaagc tgcaacttgt cgccgtggga
     7861 acgaaaatgc cggactgggt acaaaccggt tttaccgagt acctgcgtcg ttttccgaaa
     7921 gatatgccct tcgagctgat tgaaattccg gccggaaaac gcggcaagaa tgcggacatc
     7981 aagcgcatac tcgacaaaga gggtgagcag atgttggcgg ccgcaggcaa aaaccgcatt
     8041 gtcaccctcg atattccagg caagccctgg gatacaccgc agttagccgc tgagctggaa
     8101 cgctggaagc tggatggtcg cgacgtcagt ctactgattg gcgggcctga agggttgtcg
     8161 cctgcctgta aagcggcggc ggagcagagc tggtcgctgt cggcgcttac cctcccccat
     8221 ccgctggttc gcgtgctggt cgcagagagt ctgtaccggg cgtggagcat caccaccaac
     8281 catccttatc accgtgagtg ataagggagc tttgagtaga aaacgcagcg gatgaaacta
     8341 cagaactctt ttcgcgacta tacggcagag tccgcgctgt ttgtgcgccg ggcgctggtc
     8401 gcatttttgg ggattttgct gctgaccggc gtgcttatcg ccaacctgta taatctgcaa
     8461 attgttcgct ttaccgacta ccagactcgc tctaatgaaa accgcattaa gctggtgcct
     8521 atcgcgccca gccgcggcat tatctacgac cgtaatggta tccctctggc cctcaaccgc
     8581 actatctacc agatagaaat gatgccagag aaagtcgata acgtgcagca aacgctggac
     8641 gctttgcgca gcgtggttga tctgaccgat gacgatattg ctgcattccg aaaagagcgc
     8701 gcacgttcac accgtttcac ctctattccg gtgaaaacta acctgaccga agtacaagta
     8761 gctcgctttg ccgtcaatca gtaccgtttt ccgggtgtcg aagttaaagg ctataaacgt
     8821 cgttactatc cttacggttc ggcgttgacc cacgtcatcg gctatgtgtc gaaaatcaac
     8881 gataaagacg tcgaacgcct gaataatgac ggcaaactgg ccaactatgc ggcaacgcat
     8941 gatatcggta agctgggcat tgagcgttac tatgaagacg tgctgcacgg tcagaccggt
     9001 tatgaagagg ttgaagttaa caaccgtggg cgtgttatcc gccagttaaa agaagtacca
     9061 ccgcaagccg gacacgatat ttacctgacg ctggatctca aactccagca atatattgaa
     9121 acgctgctgg cgggtagccg cgcagctgtg gttgtcaccg atccgcgtac aggtggggtg
     9181 ctggcgctgg tttccacgcc tagttatgac ccaaacctgt ttgttgacgg tatctccagc
     9241 aaagattatt ccgccttgtt gaacgacccg aatacaccgc tggtgaaccg cgccacacag
     9301 ggggtttatc ctcccgcgtc tacagttaaa ccctatgtgg cggtttcggc attgagcgcc
     9361 ggggtgatca cgcgcaatac gacgctgttt gacccaggct ggtggcaact gccaggttcg
     9421 gaaaaacgtt atcgtgactg gaaaaaatgg ggccacgggc gtctgaatgt cacaagatcg
     9481 ctggaagaat ctgcggatac cttcttctat caggtggcct acgatatggg gatcgatcgc
     9541 ctctccgaat ggatgggtaa attcggttat ggtcattaca ccggtatcga cctggcggaa
     9601 gaacgttccg gcaacatgcc tacccgcgaa tggaaacaga aacgctttaa aaaaccgtgg
     9661 tatcagggtg acaccattcc ggttggtatc ggtcagggtt actggacagc gaccccaatc
     9721 cagatgagta aggcactgat gatcctgatt aatgacggta tcgtgaaggt tccgcatttg
     9781 ctgatgagca ccgccgaaga cggcaaacag gtgccatggg tacaaccgca tgaaccgccc
     9841 gtcggcgata ttcattccgg ttactgggag ctggcgaaag acggtatgta cggtgttgct
     9901 aaccgcccta acggtacggc gcataaatac tttgccagcg caccgtacaa aattgcggcg
     9961 aaatctggta ccgctcaggt cttcggtctg aaagcgaacg aaacctataa tgcgcacaaa
    10021 attgccgagc gtttacgtga ccacaaactg atgaccgcct ttgcgccata caacaatccg
    10081 caagtggctg tcgccatgat tctggagaac ggcggcgcgg gtccggcggt tggtacgctg
    10141 atgcgccaga tcctcgacca cattatgctg ggtgataaca acaccgatct gcctgcggaa
    10201 aatccagcgg ttgccgcagc ggaggaccat taatcatgac ggataatccg aataaaaaaa
    10261 cattctggga taaagtccat ctcgatccca caatgctgct gattttactg gcattgctgg
    10321 tttacagcgc cctggttatc tggagcgcca gcggtcagga tattggcatg atggagcgca
    10381 aaatcggcca aatcgcgatg ggtctggtca tcatggtggt gatggcgcaa attcctccac
    10441 gcgtttatga aggctgggcc ccctatctct atatcatctg tattattttg ctggtggcgg
    10501 tagatgcttt cggtgccatc tctaaaggtg ctcaacgctg gctggacctc ggtattgttc
    10561 gttttcagcc gtcggaaatt gccaaaatag ccgtaccact gatggttgcg cgctttatca
    10621 accgcgacgt ttgcccgcca tcgttgaaga acactggcat cgcgctggtg ctgatattta
    10681 tgcccacgct gctggtggct gcacagcctg acctgggaac atcaatcctc gttgcgcttt
    10741 ccggtctgtt tgtactgttc ctctctggcc ttagctggcg tctgattggc gtcgcagtag
    10801 tgctggtagc ggcgttcatt ccgattctgt ggttcttcct gatgcatgat taccagcgcc
    10861 agcgcgtaat gatgctcctg gacccggaat cagacccact aggcgcgggc tatcacatta
    10921 ttcagtctaa aattgctatt ggctccggcg gattacgcgg caaaggctgg ctgcacggca
    10981 ctcagtcaca gcttgaattt ctccccgaac gccataccga ctttatcttc gcggtactgg
    11041 cggaagagct gggattagtg ggcattctga ttctgctcgc tctctacatt ctgctgatca
    11101 tgcgcgggct gtggatagcc gccagagcgc aaaccacctt tggtcgcgtc atggctggtg
    11161 gcttaatgct gatattattc gtttatgtct tcgtaaatat tggtatggta agcggtattc
    11221 tgccggttgt aggggttccg ctcccactgg tcagttatgg aggatcggcg ctaattgtgc
    11281 tgatggctgg gttcgggatt gtaatgtcaa tccacaccca caggaaaatg ttgtcgaaaa
    11341 gcgtgtaaga ggtgcgcaat gcgtaagcag tggctcggga tctgcatcgc ggcaggaatg
    11401 ctcgcggcat gtacaagcga tgatggtcag caacagacgg taagtgtacc gcagcctgcg
    11461 gtatgtaacg gccccatagt tgaaattagc ggggcggacc cgcgtttcga accactgaac
    11521 gcgacggcaa atcaggatta ccagcgcgac ggtaaaagct acaaaatcgt gcaggatccg
    11581 tctcgattta gccaggcggg actggcggca atctatgatg ccgaacctgg cagtaacctg
    11641 acggcctctg gcgaagcttt cgatccgaca cagttgacgg cggcccatcc aacgcttccg
    11701 atccccagct acgccagaat cactaacctg gctaacgggc gaatgatcgt ggtgcgcatt
    11761 aatgatcgcg gcccctacgg caacgaccgc gtaatttcgc tttctcgcgc agcagctgac
    11821 cgtcttaaca cgtcaaacaa caccaaagtt cgtatcgatc cgattattgt cgcccaggat
    11881 ggttcgcttt ctggtcctgg tatggcttgt accacagtcg ccaaacagac ttacgccctg
    11941 cctgcacctc ccgatttaag cggtggcgcg ggggcaagtt cagtgtctgg cccgcagggt
    12001 gacattcttc cggtcagtaa ctcgacgcta aaaagcgaag atccgaccgg cgcgccggta
    12061 accagcagcg gtttcctcgg cgcaccaacg accttagcgc ctggtgtact ggaaagcagc
    12121 gaaccgacac ctgcaccaca gcccgttgtt acagctccgt cgacaacgcc tgcaacctcg
    12181 cctgcaatgg tgacaccgca agccgcctcg caaagcgcca gcggcaactt tatggtgcaa
    12241 gtcggggccg ttagcgatca ggctcgtgcg caacagtacc aacagcaact gggacagaag
    12301 ttcggcgtcc ccggtcgcgt aactcaaaat ggcgcggtct ggcgtatcca gcttggccca
    12361 ttcgccagta aagccgaagc cagtaccttg cagcaacgtt tgcaaaccga agcccaatta
    12421 cagtcattta ttaccaccgc gcagtagcgt aaagcaggca tctgatgtgt caatttctgt
    12481 aaatgacgtt aacaaagtca cgcggaaagt cagatgcctg ccggtagtgc atttgctata
    12541 gtagggcact ttttttaatt ccatcacgga tgtcgtagtt cagaccatga ataccatttt
    12601 ttccgctcgt atcatgaagc gcctggcgct caccacggct ctttgcacag cctttatctc
    12661 tgctgcacat gccgatgacc tgaatatcaa aactatgatc ccgggtgtac cgcagatcga
    12721 tgcggagtcc tacatcctga ttgactataa ctccggcaaa gtgctcgccg aacagaacgc
    12781 agatgtccgc cgcgatcctg ccagcctgac caaaatgatg accagttacg ttatcggcca
    12841 ggcaatgaaa gccggtaaat ttaaagaaac tgatttagtc actatcggca acgacgcatg
    12901 ggccaccggt aacccggtgt ttaaaggttc ttcgctgatg ttcctcaaac cgggcatgca
    12961 ggttccggtt tctcagctga tccgcggtat taacctgcaa tcgggtaacg atgcttgtgt
    13021 cgccatggcc gattttgccg ctggtagcca ggacgctttt gttggcttga tgaatagcta
    13081 tgttaacgcc ctgggcctga aaaacaccca cttccagacg gtacatggtc tggatgctga
    13141 tggtcagtac agctccgcgc gcgatatggc gctgatcggc caggcgttga tccgtgacgt
    13201 accgaatgaa tactcgatct ataaagaaaa agaatttacg tttaacggta ttcgccagct
    13261 gaaccgtaac ggcctgttat gggataacag cctgaatgtc gacggcatca aaaccggaca
    13321 cactgacaaa gcaggttaca accttgttgc ttctgcgact gaaggccaga tgcgcttgat
    13381 ctctgcggtg atgggcggac gtacttttaa aggccgtgaa gccgaaagta aaaaactgtt
    13441 aacctggggc ttccgtttct tcgaaaccgt taacccactg aaagtaggta aagagttcgc
    13501 ctctgaaccg gtttggtttg gtgattctga tcgcgcttcg ttaggggttg ataaagacgt
    13561 gtacctgacc attccgcgtg gtcgcatgaa agatctgaaa gccagctatg tgctgaacag
    13621 cagtgaattg catgcgccgc tgcaaaagaa tcaggtcgtc ggaactatca acttccagct
    13681 tgatggcaaa acgatcgagc aacgcccgct ggttgtgttg caagaaatcc cggaaggtaa
    13741 cttcttcggc aaaatcattg attacattaa attaatgttc catcactggt ttggttaaaa
    13801 attgaacact tgaaagtgta atttccgtcc ccatatacta agcatcagta aaaaaactcc
    13861 cgccttctgg cgggagttgc tatttaatta cgttacgccg gagctgacat gaaaaccaaa
    13921 cttaacgaac tgcttgaatt ccctactcct tttacttaca aagttatggg gcaggcgtta
    13981 cctgagctgg ttgatcaggt ggttgaagtg gtacagcgcc atgcgccagg tgactacacc
    14041 ccaacggtaa aaccaagcag caaaggcaac taccactcgg tatctatcac tatcaacgcc
    14101 actcatatcg agcaggttga aacactgtat gaagaactgg gcaaaatcga tattgtccgc
    14161 atggttctgt aactcgcttc tccgttaccc ggctcctggt cgggtaactc ccctctcctc
    14221 gctgtgatat acttttcccc cacttttact cattctccac ggagatgccg ttttgtatca
    14281 ggataaaatt cttgtccgcc agctcggtct tcagccttac gagccaatct cccaggctat
    14341 gcatgaattc accgataccc gcgatgatag tacccttgat gaaatctggc tggtcgagca
    14401 ctatccggta ttcacccaag gtcaggcagg aaaagcggag cacattttaa tgccgggtga
    14461 tattccggtg atccagagcg atcgcggtgg gcaggtgact tatcacgggc cgggacaaca
    14521 ggtgatgtat gtgttgctta acctgaaacg ccgtaaactc ggtgtgcgtg aactggtgac
    14581 cttgcttgag caaacagtgg tgaataccct ggctgaactg ggtatagaag cgcatcctcg
    14641 ggctgacgcg cctggtgtct atgtcgggga aaagaaaatt tgctcactgg gtttacgaat
    14701 tcgacgcggt tgttcattcc acggtctggc attaaacgtc aatatggatc tttcaccatt
    14761 tttacgtatt aatccttgtg ggtatgccgg aatggaaatg gctaaaatat cacaatggaa
    14821 acccgaagcg acgactaata atattgctcc acgtttactg gaaaatattt tagcgctact
    14881 aaacaatccg gacttcgaat atattaccgc ttaattccat acatcaatgg cccaatttac
    14941 actgggtcat tactcataat acctgctaat gctttacatc tccttcgcgt ttgtatattt
    15001 tctttatcac ttgcagaaat gcggtgccac gccttgctcc gtaactcacc ccattgatta
    15061 acctattgaa atagttgatt atggtgagca aaagcgttcc gttccaatta gacataaga
//
LOCUS       DBANUU010000103        15051 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000103 DBANUU010000000
VERSION     DBANUU010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.103"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..648
                     /gene="tnpA"
                     /locus_tag="ACIE68_003346"
     CDS             <1..648
                     /gene="tnpA"
                     /locus_tag="ACIE68_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFX5909611.1"
                     /translation="MKHRTWITEALRLHFEEHLPRVVAGRRLGVPKSTVCSMFVRFRR
                     AGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPRRPNFPYEFKI
                     ALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPALLPVTLTPEP
                     DNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIREIKGSSH"
     gene            648..995
                     /gene="tnpB"
                     /locus_tag="ACIE68_003347"
     CDS             648..995
                     /gene="tnpB"
                     /locus_tag="ACIE68_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFX5909612.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            1015..1416
                     /locus_tag="ACIE68_003348"
     CDS             1015..1416
                     /locus_tag="ACIE68_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909613.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALAKLRQQGREQVDESALFRMIGQMREIVTSAQKATRKARRDADRRQHLKTSAR
                     PDKPVPPDTDIADPQADNLPPAKPFDQIEEW"
     gene            1419..2279
                     /locus_tag="ACIE68_003349"
     CDS             1419..2279
                     /locus_tag="ACIE68_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HFX5909614.1"
                     /translation="MDEYPIIDLSHLLPAAQGLARLPADERIQRLRADRWIGYPRAVE
                     ALNRLEALYAWPNKQRMPNLLLVGPTNNGKSMIVEKFRRTHPASSDADQEHIPVLVVQ
                     MPSEPSVIRFYVALLAAMGAPLRPRPRLPEMEQLALALLRKVGVRMLVIDELHNVLAG
                     NSVNRREFLNLLRFLGNELRIPLVGVGTRDAYLAIRSDDQLENRFEPMMLPVWEANDD
                     CCSLLASFAASLPLRRPSPIATLDMARYLLTRSEGTIGELAHLLMAAAIVAVESGEEA
                     INHRTLSMAC"
     gene            2381..3904
                     /gene="istA"
                     /locus_tag="ACIE68_003350"
     CDS             2381..3904
                     /gene="istA"
                     /locus_tag="ACIE68_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS1326 family transposase"
                     /protein_id="HFX5909615.1"
                     /translation="MINVAILSAIRRWHFRDGASIREIARRSGLSRNTVRKYLQSKVV
                     EPQYPARDSVGKLSPFEPKLRQWLSTEHKKTKKLRRNLRSMYRDLVALGFTGSYDRVC
                     AFARQWKDSEQFKAQTSGKGCFIPLRFACGEAFQFDWSEDFARIAGKQVKLQIAQFKL
                     AHSRAFVLRAYYQQKHEMLFDAHWHAFQIFGGIPKRGIYDNMKTAVDSVGRGKERRVN
                     QRFTAMVSHYLFDAQFCNPASGWEKGQIEKNVQDSRQRLWQGAPDFQSLADLNVWLEH
                     RCKALWSELRHPELDQTVQEAFADEQGELMALPNAFDAFVEQTKRVTSTCLVHHEGNR
                     YSVPASYANRAISLRIYADKLVMAAEGQHIAEHPRLFGSGHARRGHTQYDWHHYLSVL
                     QKKPGALRNGAPFAELPPAFKKLQSILLQRPGGDRDMVEILALVLHHDEGAVLSAVEL
                     ALECGKPSKEHVLNLLGRLTEEPPPKPIPIPKGLRLTLEPQANVNRYDSLRRAHDAA"
     gene            3891..4676
                     /gene="istB"
                     /locus_tag="ACIE68_003351"
     CDS             3891..4676
                     /gene="istB"
                     /locus_tag="ACIE68_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS1326 family helper ATPase
                     IstB"
                     /protein_id="HFX5909616.1"
                     /translation="MMQHEGHVRILKSLKLFGMAHAIEELGNQNSPAFNQALPMLDSL
                     IKAEVAEREVRSVNYQLRVAKFPVYRDLVGFDFSQSLVNEATVKQLHRCDFMEQAQNV
                     VLIGGPGTGKTHLATAIGTQAVMHLNRRVRFFSTVDLVNALEQEKSSGRQGQIANRLL
                     YADLVILDELGYLPFSQTGGALLFHLLSKLYEKTSVILTTNLSFSEWSRVFGDEKMTT
                     ALLDRLTHHCHILETGNESYRFKHSSTQNKQEEKQTRKLKIET"
     gene            complement(4852..5352)
                     /locus_tag="ACIE68_003352"
     CDS             complement(4852..5352)
                     /locus_tag="ACIE68_003352"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFX5909617.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
     gene            complement(5480..6319)
                     /gene="sul1"
                     /locus_tag="ACIE68_003353"
     CDS             complement(5480..6319)
                     /gene="sul1"
                     /locus_tag="ACIE68_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFX5909618.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            complement(6313..6660)
                     /locus_tag="ACIE68_003354"
     CDS             complement(6313..6660)
                     /locus_tag="ACIE68_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFX5909619.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            complement(6844..7317)
                     /gene="dfrA14"
                     /locus_tag="ACIE68_003355"
     CDS             complement(6844..7317)
                     /gene="dfrA14"
                     /locus_tag="ACIE68_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HFX5909620.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            7476..8489
                     /gene="intI1"
                     /locus_tag="ACIE68_003356"
     CDS             7476..8489
                     /gene="intI1"
                     /locus_tag="ACIE68_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFX5909621.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            8692..9042
                     /locus_tag="ACIE68_003357"
                     /pseudo
     CDS             8692..9042
                     /locus_tag="ACIE68_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            9168..9728
                     /locus_tag="ACIE68_003358"
     CDS             9168..9728
                     /locus_tag="ACIE68_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFX5909622.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(9856..10560)
                     /locus_tag="ACIE68_003359"
     CDS             complement(9856..10560)
                     /locus_tag="ACIE68_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFX5909623.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     gene            10619..13525
                     /locus_tag="ACIE68_003360"
     CDS             10619..13525
                     /locus_tag="ACIE68_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004726714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFX5909624.1"
                     /translation="MPDDLIRHYTFNDTDLSIIRQRRGPANRLGFAVQLCYLRFPGVI
                     LGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRREHLSELQTVFGFRPFTMSHYR
                     QAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALNAVERASAEAITRANRRIYDA
                     LAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKPNSRHMLEHIERLKAWQALDL
                     PTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYATLVALATEGMATVTDEIIDL
                     HDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIGQALIDAKQSGRDAFAAIEAV
                     MSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRYAPEFLAVLKLRAAPAAKNVL
                     DAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDAGIDRRYYELCALSELKNSLR
                     SGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAVATDCEQYLHERLTLLEAQLA
                     TVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALIDQTAMVLPHVKITELLLEVD
                     EWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLTKMAESCPGTTYAKLAWLQAW
                     HTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQNFRTASKAKSTGHINPKYGS
                     SPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLYHESDLRIEEHYTDTAGFTDH
                     VFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKPMIGGTLNIKHVRAHWDEILR
                     LATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRVH
                     AGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVYLERAA
                     HALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
     gene            13592..13969
                     /locus_tag="ACIE68_003361"
     CDS             13592..13969
                     /locus_tag="ACIE68_003361"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFX5909625.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(14170..14829)
                     /locus_tag="ACIE68_003362"
     CDS             complement(14170..14829)
                     /locus_tag="ACIE68_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFX5909626.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         3376 a         4358 c         4200 g         3117 t
ORIGIN      
        1 atgaaacacc ggacctggat cactgaagct ttacgtcttc actttgaaga acatttaccc
       61 cgggttgtgg ccgggcgtcg cctgggtgta ccaaaatcaa cagtttgtag tatgttcgtg
      121 cgctttcgga gagctggcct ttcgtggcct ttgcccgcag gcatgtcgga gcaggaactt
      181 gatgcctgcc tttacggaca attttccacg gtaccagtcg tacgtcctga aagcaccgtt
      241 atatccgaag cccccgtggt aaaaaaacgt ccccggcggc ccaacttccc ttatgagttt
      301 aaaatcgcct tagtggagca gtcactgcag cccggagcct gtgtggcgca gatcgcccgg
      361 gaaaacggaa tcaacgataa cctgctcttc aactggcgcc atcaataccg gaaaggtggc
      421 ctgctgcctt ccggaaaaaa tatgccggca ctgcttcccg tgacgttaac gccggagccg
      481 gataataaaa tcccggcccc cgcacaggaa ccagagcaga taaatacacc gtccgacagt
      541 ctgtgttgtg agctggttct gccggccgga actctcaggc ttaaaggtaa actgacgccg
      601 gcgttattac agacacttat ccgcgaaata aaagggagca gccactgatg atatctctcc
      661 ctgcaggttc gcgtatctgg ctggttgcag gtatcaccga tatgcgaaat ggctttaacg
      721 gcctggcatc aaaagttcag aacgtcctga aggatgaccc gttctccgga cacctgttca
      781 tcttccgcgg acgccggggt gaccagataa aagtgttgtg ggctgacagt gacggactgt
      841 gcctcttcac caaacgcctg gagcggggcc gcttcgtctg gccagtcacc cgtgacggca
      901 aggtgcacct tactccggct cagttatcca tgcttcttga aggtatcaac tggaagcacc
      961 cgaaacgaac ggaacgcgct ggaatccgca tataacccgt tgtaaagtga ggatatggac
     1021 acctcacttg ctcatgagaa cgcccgcctg cgggcactgt tgcagacgca acaggacacc
     1081 atccgccaga tggctgaata caaccgcctg ctctcacagc gggtggcggc ttatgcttcc
     1141 gaaatcaacc ggctgaaggc gctggcgaaa ctgcggcagc aagggcgcga acaggtggat
     1201 gagtcggcgc tgttccgcat gatcggccag atgcgtgaga ttgtgaccag cgcgcagaag
     1261 gccacacgca aggcgcggcg tgacgcggat cgccgccagc acctcaagac atcagctcgg
     1321 ccggacaagc ccgttccgcc ggatacggat attgccgacc cgcaggcaga caacttgcca
     1381 cccgccaaac cgttcgacca gattgaggag tggtagccgt ggacgaatat cccatcatcg
     1441 acctgtccca cctgctgccg gcggcccagg gcttggcccg tcttccggcg gacgagcgca
     1501 tccagcgcct tcgcgccgac cgctggatcg gctatccgcg cgcagtcgag gcgctgaacc
     1561 ggctggaagc cctttatgcg tggccaaaca agcaacgcat gcccaacctg ctgctggttg
     1621 gcccgaccaa caatggcaag tcgatgatcg tcgagaagtt ccgccgcacc cacccggcca
     1681 gctccgacgc cgaccaggag cacatcccgg tgttggtcgt gcagatgccg tccgagccgt
     1741 ccgtgatccg cttctacgtc gcgctgctcg ccgcgatggg cgcgccgctg cgcccacgcc
     1801 cacggttgcc ggaaatggag caactggctc tggcactgct gcgcaaggtc ggcgtgcgca
     1861 tgctggtgat cgacgagctg cacaacgtgc tggccggcaa cagcgtcaac cgccgggaat
     1921 tcctcaacct gctgcgcttc ctcggcaacg aactgcgcat cccgttggtt ggggtaggca
     1981 cgcgcgacgc ctacctagcc atccgctccg atgaccagtt ggaaaatcgc ttcgagccga
     2041 tgatgctgcc ggtatgggag gccaacgacg attgctgctc actgctggcc agcttcgccg
     2101 cttcgctccc gctgcgccgg ccttccccaa ttgccacgct ggacatggct cgctacctgc
     2161 tcacacgcag cgagggcacc ataggggaac tggcgcactt gctgatggcg gcggccatcg
     2221 tcgccgtgga gagcggcgag gaagcgatca accatcgcac actcagcatg gcctgttgag
     2281 ttgcatctaa aattgaccca cttagggtaa agatttgcgt cgaaatttga cccacgtatg
     2341 acactgtttc ccgtctggat atggcgggag aaatcaagga gtgataaacg tggcgatatt
     2401 gagcgcaatt cgacgctggc attttcgcga tggtgcgtcg attcgggaaa tagcccgacg
     2461 aagcggcctg tccaggaaca ccgttcgcaa gtatttgcaa agcaaggtgg ttgaaccgca
     2521 gtacccagcg cgagacagcg ttggcaagtt aagtcctttt gagcccaagt taaggcagtg
     2581 gctctccacc gagcacaaaa agacaaagaa gctgcgcaga aacctgcgca gcatgtaccg
     2641 ggatttggtc gctttgggct ttaccgggtc ttatgaccga gtgtgtgcct ttgcccgaca
     2701 gtggaaagat tccgaacagt tcaaggcgca aacctcgggc aagggttgtt tcatcccctt
     2761 gcgctttgct tgtggcgaag ccttccaatt cgattggagt gaggactttg cccgcatagc
     2821 gggcaaacag gtcaaacttc agattgccca gtttaagttg gcccacagcc gggcctttgt
     2881 gcttcgggct tactaccagc aaaaacatga aatgctgttt gatgcccact ggcatgcctt
     2941 tcaaatcttc ggtggcattc ccaagcgcgg catctacgac aacatgaaga ccgctgtgga
     3001 ttcggtgggg cgtggcaaag agcgcagggt caatcagcgg ttcactgcca tggtcagcca
     3061 ctacctgttt gatgcgcagt tctgtaatcc agcatcgggt tgggagaaag gccagattga
     3121 gaagaacgtg caggattccc gccaacgcct gtggcaaggg gcaccagact ttcaaagcct
     3181 tgctgatttg aatgtgtggc ttgagcatcg ctgcaaagcg ctgtggtctg agctgcgcca
     3241 ccccgaattg gaccaaaccg tgcaagaggc ctttgccgat gaacaaggcg agttgatggc
     3301 gctacccaat gcctttgatg cattcgtgga gcaaaccaag cgagtcactt caacctgcct
     3361 tgttcaccac gagggcaatc gctacagcgt tcctgccagt tacgccaaca gggccatcag
     3421 ccttcggatt tatgcagaca agctggtgat ggctgccgaa ggccaacaca ttgccgagca
     3481 tccaagattg tttggcagtg gccacgctcg gcgtggccac acacaatacg actggcacca
     3541 ttacttgtct gtgcttcaga agaaacctgg ggcgttgcgc aatggtgcgc catttgctga
     3601 attgccaccc gcgttcaaga agcttcaatc catcttgctg caacgccccg gcggtgaccg
     3661 tgacatggtg gaaattctgg cccttgtatt gcaccacgat gaaggtgcgg tactcagtgc
     3721 tgtggaattg gcattggagt gtggcaagcc atcgaaggag catgtgctta atctgttggg
     3781 acgtttgacc gaagaacctc cacccaaacc gattccaatt cccaaggggt taaggctgac
     3841 attggaacca caggccaacg tgaaccgcta tgacagttta aggagagccc atgatgcagc
     3901 atgaaggcca tgtgagaatc ctcaaatcct tgaaactctt tggcatggca cacgccattg
     3961 aggagttggg caatcagaat tcaccagcat ttaatcaagc cttgcccatg ctggacagct
     4021 tgattaaagc tgaagtggca gagcgtgaag tacgttcggt gaactatcaa ttgcgggtgg
     4081 ccaagttccc cgtgtatcgg gacttggtgg gctttgactt cagtcaaagc ctggttaatg
     4141 aggccacggt caaacaattg caccggtgcg acttcatgga acaagcccag aacgtggtgc
     4201 tgattggtgg gccaggcaca ggcaagactc acctggccac agccattggt acacaagcag
     4261 tgatgcactt gaaccgacgg gtgcgtttct tctccaccgt ggatttggtc aatgcactgg
     4321 agcaagagaa atcatctggg cgtcagggac aaatcgcaaa ccgtctgttg tatgccgatt
     4381 tggtgattct ggatgagctg ggatatttgc cttttagcca aaccggtggg gcactgctgt
     4441 ttcacctgct ctcaaagctg tacgaaaaaa ccagcgtgat actgaccacc aacttgagct
     4501 tctcggaatg gagccgagtg tttggcgatg aaaagatgac aacagcgttg ttggaccgac
     4561 taacccacca ctgccacatc ctggaaaccg gcaatgaaag ttaccgcttc aaacacagtt
     4621 caactcagaa taagcaggag gaaaaacaga cccgcaaact gaaaatcgag acataattct
     4681 gacaacaagg ggtgggtcaa aattcaatgc aaatcccggg tcaaatttgg gtgcaaatca
     4741 acagatatcg acaacctctc gcgcaaccaa gacatcgcgg tcggactgca agtgatcttg
     4801 aagccacggg cccgtcccac cccgacatgg acctcgatgc ccgaacggac gttagatttc
     4861 gagttctagg cgttctgcga tgaaggttgg atcccagccg ggattgaaag tgtcgacgtg
     4921 ggtgaatccg agccgctcgt ataggccacg caggttcggg tggcagtcga gccgcagctt
     4981 ggcgcacccc tgcgttcgcg cggcatggcg gcaagcctcg atcagcgcgg agctgacacc
     5041 ccggcccgca tgtgtccgtc gcaccgcgag cttgtgcaga tatgcggcct cccccttgag
     5101 ggcgtcgggc cagaactcgg gatcctcggc cgacaaggtg caacagccga cgatgccgtc
     5161 gctgcaactc gcgactagga gctcggatct caggacgaag gtctccgcga atgtccggtc
     5221 gatccgcgcg acgtcccagg cgggcgttcc cttggcggac atccacgccg cagcgtcgtg
     5281 catcagccgc acaacctcgt cgatatcacc cgagcaggcg acccgaacgt tcggaggctc
     5341 ctcgctgtcc attcgctccc ctggcgcggt atgaaccgcc gcctcatagt gcagtttgat
     5401 cctgacgagc ccagcatgtc tgcgcccacc ttcgcggaac ctgaccaggg tccgctagcg
     5461 ggcggccgga aggtgaatgc taggcatgat ctaaccctcg gtctctggcg tcgcgactgc
     5521 gaaatttcgc gagggtttcc gagaaggtga ttgcgcttcg cagatctcca ggcgcgtggg
     5581 tgcggacgta gtcagcgcca ttgccgatcg cgtgaagttc cgccgcaagg ctcgctggac
     5641 ccagatcctt tacaggaagg ccaacggtgg cgcccaagaa ggatttccgc gacaccgaga
     5701 ccaatagcgg aagccccaac gccgacttca gcttttgaag gttcgacagc acgtgcagcg
     5761 atgtttccgg tgcggggctc aagaaaaatc ccatccccgg atcgaggatg agccggtcgg
     5821 cagcgacccc gctccgtcgc aaggcggaaa cccgcgcctc gaagaaccgc acaatctcgt
     5881 cgagcgcgtc ttcgggtcga aggtgaccgg tgcgggtggc gatgccatcc cgctgcgctg
     5941 agtgcataac caccagcctg cagtccgcct cagcaatatc gggatagagc gcagggtcag
     6001 gaaatccttg gatatcgttc aggtagccca cgccgcgctt gagcgcatag cgctgggttt
     6061 ccggttggaa gctgtcgatt gaaacacggt gcatctgatc ggacagggcg tctaagagcg
     6121 gcgcaatacg tctgatctca tcggccggcg atacaggcct cgcgtccgga tggctggcgg
     6181 ccggtccgac atccacgacg tctgatccga ctcgcagcat ttcgatcgcc gcggtgacag
     6241 cgccggcggg gtctagccgc cggctctcat cgaagaagga gtcctcggtg agattcagaa
     6301 tgccgaacac cgtcaccatg gcgtcggcct ccgcagcgac ttccacgatg gggatcgggc
     6361 gagcaaaaag gcagcaatta tgagccccat acctacaaag ccccacgcat caagcttttg
     6421 cccatgaagc aaccaggcaa tggctgtaat tatgacgacg ccgagtcccg accagactgc
     6481 ataagcaaca ccgacaggga tggatttcag aaccagagaa agaaaataaa atgcgatgcc
     6541 ataaccgatt atgacaacgg cggaaggggc aagcttagta aagccctcgc tagattttaa
     6601 tgcggatgtt gcgattactt cgccaactat tgcgataaca agaaaaagcc agcctttcat
     6661 gatatatctc ccaatttgtg tagggcttat tatgcacgct taaaaataat aaaagcagac
     6721 ttgacctgat agtttggctg tgagcaatta tgtgcttagt gcatctaacg tcgctatcaa
     6781 tcgcggaaaa aggcgtagtg cgcgaagcga acgaagcttt ttgccgtcga ttgcatagct
     6841 ttgttaaccc tttttccaaa tttgatagca atagttaatg tttgaagtaa agtgttgctc
     6901 aaaaacaact tcgaaggtat ttggaatact cgggaagaaa acatccccct ctggctcgat
     6961 gtcgatcgtc gataagtgga gcgtagaggc catgggtaat gtttctcggt aaatttctcc
     7021 gccaccagac actataacgt gaccggtgaa ttcagctagc ctgtccatgg cctcttcgat
     7081 tgactgaaat acaactacat tgtcatcatt tgatgtccaa cctgagcggg taacgaccgc
     7141 gtatttccta ttggggagtg cgcccataga ttcaaacgtc ttgcgaccca ccagaagcca
     7201 ctgattgtag gtcaatgctt taaaaagtag ctgctcccct ttcgcggacc agggtatgtc
     7261 tggaccgcaa ccaatcacgc cgtttttcgc tttcgcagct atcaatgata ctttcaaggt
     7321 tctcatcctg ggttaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc
     7381 tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca
     7441 tagactgtac cccaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac
     7501 caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc tacttgcatt
     7561 acagcttacg aaccgaacag gcttatgtcc actgggttcg tgccttcatc cgtttccacg
     7621 gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcga ggcatttctg tcctggctgg
     7681 cgaacgagcg caaggtttcg gtctccacgc atcgtcaggc attggcggcc ttgctgttct
     7741 tctacggcaa ggtgctgtgc acggatctgc cctggcttca ggagatcgga agacctcggc
     7801 cgtcgcggcg cttgccggtg gtgctgaccc cggatgaagt ggttcgcatc ctcggttttc
     7861 tggaaggcga gcatcgtttg ttcgcccagc ttctgtatgg aacgggcatg cggatcagtg
     7921 agggtttgca actgcgggtc aaggatctgg atttcgatca cggcacgatc atcgtgcggg
     7981 agggcaaggg ctccaaggat cgggccttga tgttacccga gagcttggca cccagcctgc
     8041 gcgagcagct gtcgcgtgca cgggcatggt ggctgaagga ccaggccgag ggccgcagcg
     8101 gcgttgcgct tcccgacgcc cttgagcgga agtatccgcg cgccgggcat tcctggccgt
     8161 ggttctgggt ttttgcgcag cacacgcatt cgaccgatcc acggagcggt gtcgtgcgtc
     8221 gccatcacat gtatgaccag acctttcagc gcgccttcaa acgtgccgta gaacaagcag
     8281 gcatcacgaa gcccgccaca ccgcacaccc tccgccactc gttcgcgacg gccttgctcc
     8341 gcagcggtta cgacattcga accgtgcagg atctgctcgg ccattccgac gtctctacga
     8401 cgatgattta cacgcatgtg ctgaaagttg gcggtgccgg agtgcgctca ccgcttgatg
     8461 cgctgccgcc cctcactagt gagaggtagg gcagcgcaag tcaatcctgg cggattcact
     8521 acccctgcgc gaaggccatc ggtgccgcat cgaacggccg gttgcggaaa gtcctccctg
     8581 cgtccgctga tggccggcag cagcccgtcg ttgcctgatg gatccaaccc ctccgctgct
     8641 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac aatggaggtg
     8701 gtagccgagg gtgtggaaac acccgactgc cttgcgtggt tgcggcaggc gggttgcgac
     8761 acggtgcagg gtttcctgtt cgccaggccg atgccggcgg cggccttcgt cggcttcgtc
     8821 aaccaatgga ggaacaccac catgaacgcc aatgaaccga gcaccagttg ctgcgtgtgc
     8881 tgcaaggaaa tcccgctcga tgccgccttc acgccggaag gggccgagta cgtggagcat
     8941 ttctgcgggc tggagtgcta tcagcgcttc caggcgcggg ccagcactgc gaccgaaacc
     9001 agcgtcaaac cggacgcttg tgattcgccg ccgtcaggtt gaggcatacc ctaacctgat
     9061 gtcagatgcc atgtgtaaat tgcgtcagga taggattgaa ttttgaattt attgacatat
     9121 ctcgttgaag gtcatagagt cttccctgac attttgcagg gaattccatg actggacagc
     9181 gcattgggta tatcagggtc agcaccttcg accagaaccc ggaacggcaa ctggaaggcg
     9241 tcaaggttga tcgcgctttt agcgacaagg catccggcaa ggatgtcaag cgtccgcaac
     9301 tggaagcgct gataagcttc gcccgcaccg gcgacaccgt ggtggtgcat agcatggatc
     9361 gcctggcgcg caatctcgat gatttgcgcc ggatcgtgca aacgctgaca caacgcggcg
     9421 tgcatatcga attcgtcaag gaacacctca gttttactgg cgaagactct ccgatggcga
     9481 acctgatgct ctcggtgatg ggcgcgttcg ccgagttcga gcgcgccctg atccgcgagc
     9541 gtcagcgcga gggtattgcg ctcgccaagc aacgcggggc ttaccgtggc aggaagaaat
     9601 ccctgtcgtc tgagcgtatt gccgaactgc gccaacgtgt cgaggctggc gagcaaaaga
     9661 ccaagcttgc tcgtgaattc ggaatcagtc gcgaaaccct gtatcaatac ttgagaacgg
     9721 atcagtaaat atgccacgtc gttccatcct gtccgccgcc gagcgggaaa gcctgctggc
     9781 gttgccggac tccaaggacg accggcactg ttgcaaagtt agcgatgagg cagccttttg
     9841 tcttattcaa aggccttaca tttcaaaaac tctgcttacc aggcgcattt cgcccagggg
     9901 atcaccataa taaaatgctg aggcctggcc tttgcgtagt gcacgcatca cctcaatacc
     9961 tttgatggtg gcgtaagccg tcttcatgga tttaaatccc agcgtggcgc cgattatccg
    10021 tttcagtttg ccatgatcgc attcaatcac gttgttccgg tacttaatct gtcggtgttc
    10081 aacgtcagac gggcaccggc cttcgcgttt gagcagagca agcgcgcgac cataggcggg
    10141 cgctttatcc gtgttgatga atcgcgggat ctgccacttc ttcacgttgt tgaggatttt
    10201 acccagaaac cggtatgcag ctttgctgtt acgacgggag gagagataaa aatcgacagt
    10261 gcggccccgg ctgtcgacgg cccggtacag atacgcccag cggccattga ccttcacgta
    10321 ggtttcatcc atgtgccacg ggcaaagatc ggaagggtta cgccagtacc agcgcagccg
    10381 tttttccatt tcaggcgcat aacgctgaac ccagcggtaa atcgtggagt gatcgacatt
    10441 cactccgcgt tcagccagca tctcctgcag ctcacggtaa ctgatgccgt atttgcagta
    10501 ccagcgtacg gcccacagaa tgatgtcacg ctgaaaatgc cggcctttga atgggttcat
    10561 gtgcagctcc atcagcaaaa ggggatgata agtttatcac caccgactat ttgcaacagt
    10621 gccggacgac ctgatccgac attacacatt caacgatacc gacctctcga tcatccgaca
    10681 gcggcgcggg ccagccaatc ggctgggctt cgcggtgcag ctctgttacc tgcgctttcc
    10741 cggcgtcatc ctgggcgtcg atgaactacc gttcccgccc ttgttgaagc tggtcgccga
    10801 ccagctcaag gtcggcgtcg aaagctggaa cgagtacggc cagcgggagc agacccggcg
    10861 cgagcacctg agcgagctgc aaaccgtgtt cggtttccgg cccttcacca tgagccatta
    10921 ccggcaggcc gtccagatgc tgaccgagct ggcgatgcaa accgacaaag gcatcgtgct
    10981 ggccagcgcc ttgatcgggc acctgcggcg gcagtcggtc attctgcccg ccctcaacgc
    11041 cgtcgagcgg gcgagtgccg aggcgatcac ccgtgctaac cggcgcatct acgacgcctt
    11101 ggccgaacca ctggcggacg cgcatcgccg ccgcctcgac gatctgctca agcgccggga
    11161 caacggcaag acgacctggt tggcttggtt gcgccagtct ccggccaagc caaattcgcg
    11221 gcatatgctg gaacacatcg aacgcctcaa ggcatggcag gcactcgatc tgcctaccgg
    11281 catcgagcgg ctggttcacc agaaccgcct gctcaagatt gcccgcgagg gcggccagat
    11341 gacacccgcc gacctggcca aattcgagcc gcaacggcgc tacgccactc tcgtggcgct
    11401 ggccaccgag ggcatggcca ccgtcaccga cgaaatcatc gacctgcacg accgcatcct
    11461 gggtaagctg tttaacgctg ccaagaataa gcatcagcag cagttccagg cgtcaggcaa
    11521 ggccatcaac gccaaggtac gtctgtacgg gcgcatcggt caggcgctga tcgacgccaa
    11581 gcaatcaggc cgcgatgcgt ttgccgccat cgaggccgtc atgtcctggg attcctttgc
    11641 cgagagcgtc accgaggcgc agaagctcgc gcaacccgat gacttcgatt tcctgcatcg
    11701 catcggcgag agctacgcca ccctgcgccg ctatgcaccg gaattccttg ccgtgctcaa
    11761 gctgcgggcc gcgcccgccg ccaaaaacgt gcttgatgcc attgaggtgc tgcgcggcat
    11821 gaacaccgac aacgcccgca agctgccagc cgatgcaccg accggcttca tcaagccgcg
    11881 ctggcagaaa ctggtgatga ccgacgccgg catcgaccgg cgctactacg aactgtgcgc
    11941 gctgtccgag ttgaagaact ccctgcgctc gggcgacatc tgggtgcagg gttcacgcca
    12001 gttcaaggac ttcgaggact acctggtacc gcccgagaag ttcaccagcc tcaagcagtc
    12061 cagcgaattg ccgctggccg tggccaccga ctgcgaacaa tatctgcatg agcggctgac
    12121 gctgctggaa gcacaacttg ccaccgtcaa ccgcatggcg gcagccaacg acctgccgga
    12181 tgccatcatc accgagtcgg gcttgaagat cacgccgctg gatgcggcgg tgcccgacac
    12241 cgcgcaggcg ctgatagacc agacagccat ggtcctgccg cacgtcaaga tcaccgaact
    12301 gctgctcgaa gtcgatgagt ggacgggctt cacccggcac ttcacgcact tgaaatcggg
    12361 cgatctggcc aaggacaaga acctgttgtt gaccacgatc ctggccgacg cgatcaacct
    12421 gggcctgacc aagatggccg agtcctgccc cggcacgacc tacgcgaagc tcgcttggct
    12481 gcaagcctgg catacccgcg acgaaacgta ctcgacagcg ttggctgaac tggtcaacgc
    12541 tcagtttcgg catccctttg ccgggcactg gggcgatggc accacatcat catcggacgg
    12601 acagaatttc cgaaccgcta gcaaggcaaa gagcacgggg cacatcaacc caaaatatgg
    12661 cagcagccca ggacggactt tctacaccca catctccgac caatacgcgc cattccacac
    12721 caaggtggtc aatgtcggcc tgcgcgactc aacctacgtg ctcgacggcc tgctgtacca
    12781 cgaatccgac ctgcggatcg aggagcacta caccgacacg gcgggcttca ccgatcacgt
    12841 cttcgccctg atgcacctct tgggcttccg cttcgcgccg cgcatccgcg acctgggcga
    12901 caccaagctc tacatcccga agggcgatgc cgcctatgac gcgctcaagc cgatgatcgg
    12961 cggcacgctc aacatcaagc acgtccgcgc ccattgggac gaaatcctgc ggctggccac
    13021 ctcgatcaag cagggcacgg tgacggcctc gctgatgctc aggaaactcg gcagctaccc
    13081 gcgccagaac ggcttggccg tcgcgctgcg cgagttgggc cgcatcgagc gcacgctgtt
    13141 catcctcgac tggctgcaaa gcgtcgagct acgccgccgc gtgcatgccg ggctgaacaa
    13201 gggcgaggcg cgcaatgcgc tggcccgtgc cgtgttcttc aaccgccttg gtgaaatccg
    13261 tgaccgcagt ttcgagcagc agcgctaccg ggccagcggc ctcaacctgg tgacggcggc
    13321 catcgtgctg tggaacacgg tctacctgga gcgtgcggcg catgcgttgc gcggcaatgg
    13381 tcatgccgtc gatgactcgc tattgcagta cctgtcgcca ctcggctggg agcacatcaa
    13441 cctgaccggt gattacctat ggcgcagcag cgccaagatc ggcgcgggga agttcaggcc
    13501 gctacggcct ctgcaaccgg cttagcgtgc tttattttcc gttttctgag acgaccccta
    13561 ttatggcgaa ggtatcattc aggaagcgtt gatgaaggat tatctctgta aaaagttatt
    13621 caaccggctt tccggtaccc tggtgatcag agcgcgctgc ggcaataaca ttactggcct
    13681 ggcatgttgc aatattcttt atccctcgcc ccgatacagc ggtcagctgc atattaaaga
    13741 gctgtatgtt tctcagtgcg atcgaaataa gggcacgggt aaagcgataa tgcgctttat
    13801 agcccggctt gcgcttgaac aggaatgcct tagccttagc tggaacgctg aaaaatccaa
    13861 ccccggcgct aaccgttttt atcaggctct gggaggcaga ataaatgatc atatcgtcaa
    13921 ttattacctc cacggggaga gcctgagcaa actggcctca ggcatttgag aagcacacgg
    13981 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
    14041 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat
    14101 ccgcttatta tcacttattc aggcgtagca accaggcgtt taagggcacc aataactgcc
    14161 ttaaaaaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
    14221 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
    14281 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
    14341 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
    14401 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
    14461 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
    14521 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
    14581 caccagctca ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc
    14641 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
    14701 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
    14761 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
    14821 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
    14881 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca
    14941 ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta
    15001 ttctgcgaag tgatcttccg tcacaggtat ttattcggcg caaagtgcgt c
//
LOCUS       DBANUU010000104        14991 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000104 DBANUU010000000
VERSION     DBANUU010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.104"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(59..2338)
                     /gene="maeB"
                     /locus_tag="ACIE68_003363"
     CDS             complement(59..2338)
                     /gene="maeB"
                     /locus_tag="ACIE68_003363"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFX5909627.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2627..3577
                     /gene="tal"
                     /locus_tag="ACIE68_003364"
     CDS             2627..3577
                     /gene="tal"
                     /locus_tag="ACIE68_003364"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFX5909628.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3597..5600
                     /gene="tkt"
                     /locus_tag="ACIE68_003365"
     CDS             3597..5600
                     /gene="tkt"
                     /locus_tag="ACIE68_003365"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFX5909629.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5696..6739)
                     /gene="ypfG"
                     /locus_tag="ACIE68_003366"
     CDS             complement(5696..6739)
                     /gene="ypfG"
                     /locus_tag="ACIE68_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFX5909630.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPQASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6865..7440)
                     /gene="nudK"
                     /locus_tag="ACIE68_003367"
     CDS             complement(6865..7440)
                     /gene="nudK"
                     /locus_tag="ACIE68_003367"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFX5909631.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(7508..9487)
                     /gene="aegA"
                     /locus_tag="ACIE68_003368"
     CDS             complement(7508..9487)
                     /gene="aegA"
                     /locus_tag="ACIE68_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFX5909632.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDNIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9693..11393
                     /gene="narQ"
                     /locus_tag="ACIE68_003369"
     CDS             9693..11393
                     /gene="narQ"
                     /locus_tag="ACIE68_003369"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFX5909633.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11557..14670
                     /gene="acrD"
                     /locus_tag="ACIE68_003370"
     CDS             11557..14670
                     /gene="acrD"
                     /locus_tag="ACIE68_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFX5909634.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14769..14828)
                     /gene="ypfM"
                     /locus_tag="ACIE68_003371"
     CDS             complement(14769..14828)
                     /gene="ypfM"
                     /locus_tag="ACIE68_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFX5909635.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3517 a         3980 c         3915 g         3579 t
ORIGIN      
        1 attgcagcca aaccgcttac cctgaatatt cagggtaagc gtgagagtta aaaaagaatt
       61 acagcggttg ggtttgcgct tcaaccacgg ccagcgccac catgttgacg atacgacgca
      121 ccgatgcgat cggcgttaac acgtgaaccg gtttcgccac gcccatcagc accggaccga
      181 cagtcacacc ttccgagctg gaaacgcgca gtaagttgta actaatgcgg gcagcttcca
      241 tgttcggcat caccagaata ttggcagaac ctttcaaagg gctgtccggc atacggtcgt
      301 tgcgaatcgc ttccaccagc gctgcatcgc cgtgcatttc accatcaatc atcagatctg
      361 gcgcacgatc cctgaccagt tccagcgcct gacgcatttt gctcgacgac gggcagtcag
      421 aagaaccaaa gttggagtgc gacaacagag caacgcgcgg ctcaatacca aaacgacgga
      481 cagtttctgc cgccatcaag gtgatctccg ccagctcttc tgcgtctggt tcgtcattga
      541 cgtaggtatc ggcaataaag gtgttaccac tcggcagcag cagcgcgttc attgcacctg
      601 cggtgtgaac gccatcgcga taaccaaaga catttttcac cacgctaaaa tgttcatgat
      661 aatcgcccac cgtaccgcag atcattgcat cggcttcccc acgctgaacc atgatcgcgc
      721 cgatcactgt cgggttactg atcagcgccc gctgcgcctg ttcctgagtg acaccgcgac
      781 gcttcatgat ctggaagtat tcggtccagt actctttaaa gcgcggatcg gattcgttat
      841 tgacgatctc aaaatcaacg cccgctttga tctgcaagcc cagtttctga atgcgcattt
      901 cgatcacgtt cggacgaccg ataaggatcg gtttcgccag tcccagcgtt accagttcct
      961 gagtggcatg cagaacgcgc gcctcttccc cttctggcag aacaacgcgc ttcggcgctt
     1021 tgcgagcctg ggagaaaatc ggcttcataa acaggttggt tttgtaaacg aactcagtca
     1081 gcttgtcgat gtagacgtcg aaatcagcaa tcggacgcgt cgccacgccc gactccatcg
     1141 cggctttagc gaccgcagga gcgatcttaa cgatcaagcg cggatcaaac ggttttggaa
     1201 tgatgtattc cggaccaaag ctcagatcct gatcgccata cgctgaagcc accacttcgc
     1261 tctgttcggc gtgggccagt tccgcaatcg cgcgtaccgc cgccagtttc atctcttcat
     1321 tgatagccgt tgcgccaaca tccagcgcgc cacggaagat aaacgggaag caaaggacgt
     1381 tgttgacctg gtttggatag tcagaacgac cggtgcaaat gatggcatcc ggacgcactt
     1441 ctttcgccag cggcggcaga atttccggtt ccgggttcgc cagcgccagg atcattggcg
     1501 cacgagccat tttcttcacc atttcctggg tcagcacttt cgggccggaa catccaagga
     1561 aaatatccgc gccttcaatc acatcatcga gggtacgttt gccgtcatcc accaccgcat
     1621 atgcggcttt ggtttccgcc atgtttggct cacggccctg atagataacg ccttttgaat
     1681 cgcaaaccac gatgttatgt ttttgcagac ccagcgctac cagcaggttc atacaggcga
     1741 ttgctgcggc acccgcgccg gaaaccacca tccgcacgtc ggagatgttt ttctccacca
     1801 cgcgcaagcc gttgaggatg gcggcagtgc tgatgattgc cgtgccgtgc tgatcgtcgt
     1861 ggaataccgg aatattcatc cgctcgcgca gtttctgttc aatatagaaa cattctggcg
     1921 ctttaatgtc ttcgaggttg attccgccga aggtcggttc gagcgcagcg acaacttcaa
     1981 taaatttgtc cgggtcgagt tcatcaactt caatgtcaaa tacatcaatc ccggcgaatt
     2041 tcttaaacag aacgcccttg ccttccatca ccggtttgcc agccagcgcg ccaatgttgc
     2101 ctaaccccag caccgccgta ccgttagaga tcaccgccac caggttaccg cgcgcggtat
     2161 atttgtaggc ttttaacggg tctttttcga tttcaagaca aggtgcggca acgcctggtg
     2221 agtaggccag cgccagatcg cgctgcgttg ccagaggctt ggttggagaa acctggattt
     2281 tccctggaac tggaaattca tggaaatcaa gtgcactttg ttttaactgg tcatccattt
     2341 ggttgttcct ttcacgtaac gttcacaaat aaagtgtgtg ggcaacagcc cctgcccaca
     2401 acgtggcgca cattattacc ctgccggagt ctacagactt tgagcaagtc caaactctca
     2461 ccattaatat aatgttttgg taataatcct ataacactga tgttacctgc ttaatccagc
     2521 aataccatgc ctgtctgcta tgcttttttg atgcgtttag cgaaatttct cagaagtgtg
     2581 aattaacgca ctcatctaac actttacttt tcaaggagta tttcctatga acgagttaga
     2641 cggcatcaaa cagttcacca ctgtcgtggc agacagcggc gatattgagt ccattcgcca
     2701 ttatcatccc caggatgcca ccaccaatcc ttcgctgtta ctcaaagctg ccggattatc
     2761 acaatatgag catttaatag acgatgctat cgcctggggt aaaaaaaatg gcaagaccca
     2821 ggaacaacag gtggtcgcag cgtgtgacaa actggcagtc aatttcggtg ctgaaattct
     2881 caaaatcgta cccggtcgcg tgtcaacaga agttgatgca cgcctctctt ttgataaaga
     2941 aaagagtatt gagaaggcgc gccatctggt ggacttgtat cagcaacaag gcgttgagaa
     3001 atcacgcatt ttgatcaaac tggcttcgac ctgggaagga attcgcgcgg ccgaagagct
     3061 ggaaaaagaa ggtattaact gcaacctgac gctgctgttt tcttttgccc aggcgcgggc
     3121 ctgtgcagaa gcgggcgttt ttctgatttc gccgtttgtc gggcgtattt atgactggta
     3181 tcaggcacgc aagccgatgg acccgtatgt ggtggaagaa gatccgggcg ttaaatcggt
     3241 gcgcaatatc tacgactact ataagcaaca ccactatgaa accattgtga tgggcgcgag
     3301 cttccgtcgc accgaacaaa tcctcgcctt aaccggctgc gatcgactga ctatcgcacc
     3361 gaatttgctg aaggagctgc aggaaaaagt ttcgccagtg gtacgtaaat taatcccgcc
     3421 ttctcagacg ttcccacgcc cagcacccat gagcgaagcg gagttccgtt gggagcacaa
     3481 tcaggatgcg atggcggtag aaaaactgtc tgaaggcatt cgtctattcg ccgttgatca
     3541 acgcaaactg gaagatcttc ttgccgccaa actataaacc agccacggag tgttatatgt
     3601 cccgaaaaga ccttgccaat gcgattcgcg cactcagtat ggatgcggta caaaaagcca
     3661 attctggtca tcccggcgcg ccgatgggca tggctgatat tgccgaagtg ctgtggaacg
     3721 attttcttaa acataatcct accgacccaa cctggtatga tcgcgaccgc tttattcttt
     3781 ccaacggtca cgcgtcgatg ctgctctaca gtttgctaca tctgaccggt tacgacctgc
     3841 cgctggaaga actgaagaac ttccgtcagt tgcattcgaa aaccccaggc cacccggaga
     3901 ttggctatac gccaggcgtt gaaaccacca ccggcccgct tggacaaggt ttggcgaacg
     3961 ccgtcgggct ggcgatagcg gaacgtacac tggcggcgca gtttaaccag ccagaccatg
     4021 agatcgtcga tcacttcacc tatgtgttta tgggcgacgg ctgcctgatg gaaggtattt
     4081 cccacgaagt ctgttcgctg gcaggcacgc tgggactggg caagctgatt ggtttttacg
     4141 atcacaacgg tatttccatc gacggtgaaa cagaaggctg gtttaccgac gatacggcaa
     4201 aacgttttga agcctatcac tggcatgtga tccatgaaat cgacggtcac gatccgcagg
     4261 cggtgaagga agcgatcctt gaagcgcaaa gcgtgaaaga taagccgtcg ctgattatct
     4321 gccgtacggt gattggcttt ggttcgccga ataaagcagg taaggaagag gcgcacggcg
     4381 caccactggg ggaagaagaa gtggcgctgg cacggcaaaa actgggctgg caccatccgc
     4441 catttgagat ccctaaaaag atttatcacg cctgggatgc ccgtgaaaaa ggcgaaaaag
     4501 cgcagcagag ctggaatgag aagtttgccg cctataaaaa ggctcatccg caactggcag
     4561 aagagtttac ccgacggatg agcggtggtt taccgaagga ctgggagaaa acgactcaga
     4621 aatatatcaa tgagttacag gcaaatccgg cgaaaatcgc tacccgtaag gcttcgcaaa
     4681 atacgcttaa cgcttacggg ccgatgctgc ctgagttgct cggcggttcg gcggatctgg
     4741 ctcccagcaa cctgaccatc tggaaaggtt ctgtttcgct gaaggaagat ccagcgggca
     4801 actacattca ctacggggtg cgtgaatttg gcatgaccgc tatcgccaac ggcatcgcgc
     4861 accacggcgg ctttgtgccg tataccgcga cgttcctgat gtttgttgaa tacgcccgta
     4921 acgccgcgcg gatggcggca ctgatgaaag cgcggcagat tatggtttat acccacgact
     4981 caattggcct gggcgaagat ggtccgacgc accaggctgt tgagcaactg gccagcctgc
     5041 gcttaacgcc aaatttcagc acctggcgac cgtgcgatca ggtggaagcg gcggtgggct
     5101 ggaagctggc ggttgagcgc cacaacggac cgacggcact gatcctctca aggcagaatc
     5161 tggcccaggt ggaacgtacg ccggatcagg ttaaagagat tgctcgtggc ggttatgtgc
     5221 tgaaagacag cggcggtaag ccagatatta ttctgattgc caccggttca gagatggaaa
     5281 ttaccctgca agcggcagag aaattagcag gagaaggtcg caatgtacgc gtagtttccc
     5341 tgccctcgac cgatattttc gacgcccagg atgaggaata tcgggagtcg gtgttgcctt
     5401 ctaacgttgc ggctcgcgtg gcggtggaag caggtattgc cgattactgg tacaagtatg
     5461 ttggtctgaa aggggcaatt gtcgggatga cgggttacgg ggaatctgct ccggcggata
     5521 agctgttccc gttctttggc tttaccgccg agaatattgt ggcaaaagcg cataaggtgc
     5581 tgggagtgaa aggtgcctga tggtgatcgc cggatgctga ttgccggatg cgacgctgac
     5641 gcgtcttatc cggcctacag cgtcttatcc ggcctacatg tccccgcaat tttgtttaac
     5701 gggtgatcca caacgtgggc caggcgtctg gcccatgcca gttatcgcag gtgggttctg
     5761 cggcataacg caccaggcga aaacgctgac cgtcaaagcg ccagcgcgcc tgaatgccac
     5821 aatcgcttaa tccgcgccct ttcgctaagg tcaccagttc acgcgatttc tcatcaaatg
     5881 ttgcgttcat cagttccagt tcattcgtct cctgaccgtt gttgaacggc aaacgcaacc
     5941 gaaccgggcg cgaggcttgt ggctttttac gcgacacaat ccatgccaaa tcaatggtgt
     6001 tataggcccc tgcctcacag ctaatcatca tcagcgcttt atcatcagtc agcgcagtga
     6061 cattcacctc acgacgtaac ggatcaagcg agcagcgcag accattcatc cgccagtttc
     6121 cataatccag caaatcgttg cgttcttcga gtgagagtgg cgtcggcgtt gggttaacca
     6181 ccgcgacctc tttcagcgca ggcgcgggcg gtacgctgag cggcggttcg tcccctttct
     6241 tgatccacgc ggtttcactg ccaacgcgct tttgctgagc atcaataaac aacaacgccg
     6301 cttttaagcc actcagagaa atggtctgat tgccgtcgcg taaggtgatc gccttccctt
     6361 cctgaatcat ctgcaaaaac gcggtgatgg ttgccgtatc atcggttact aataaccatg
     6421 gtgaaatccg ccacttgtca ccacttaacg ccaacggctc accatctaac agcagccgtg
     6481 gcgctatctc tccttcagac gcatccggcg acttcaaccc gccgcgctca atacgtaaaa
     6541 cggcatcggt atgcgccccg gcgctgcggc tcagggtcat caccagtcca ttatgatcgc
     6601 ccgtattgcg cgccacgcag aagttttggt tattacaggt gacctgccag tcggaaaacg
     6661 cccgttgcgc tggtgccgcc cacaccaaag acgtcggcaa caaagcgaaa aaaaagagaa
     6721 ggaaaatgcg atagcgcatg gacggtacga ccccagaagt tcaaacaaac agcacaagtc
     6781 gtatcttcgt tgcgcaggta gcgcggctca atcagatttg tcggattgag ccaataataa
     6841 acaacgaaat attattgtta tttttcagtc cattaaatgt gacgtttgca aatagttaag
     6901 caataacacc gtcttaccgt cacgtatctc gccggttttg atcatctcca gcgcctggct
     6961 gaacggcagc tcgagcactt caatatcttc atcttcaaca ccgccaccgg cgttagcgcg
     7021 ctgattgtca ctgtattcgg cgataaaaaa gtggattagc tcagtcacac cgccaggcga
     7081 catatacagt tcaaataatt tgcgcacttc gcccacttca tagcccgtct cttcaatcgc
     7141 ttctttacga atgcacactt ccggttcgtc gttatccagc agcccggcgc aggtttcaat
     7201 cagctgcccg ctttcattgc cattaaccca ggtagcgaca cggaactgac gaatcagaac
     7261 cacggtcttt ttcttcgcgt tgtacaggag gatcgtcgca ccattaccgc gatcgtaaac
     7321 ttcacgttta tggcggataa cttcgccatc tttgcgcgtg agatcgtaag taatgttgtg
     7381 cagggtgaaa tagttatcgg agagaatttt gtctttaatg agggtgattt gttgcgtcat
     7441 agcgactcca cagcgcgaaa tgaacaatta tcttacgctg tgaagtcggg tttgtctgcg
     7501 caggctatca gtgagatttg actgatttta cccccagcca gtcaataatg ccctgtgccg
     7561 catgacgtcc ttctgccatt gcggtaacca ccagatccgc accacgcacg gcgtcgccac
     7621 cggcgaagat tttcggattg gtggtctggt aacggtactg gctttccaca tccgcgatga
     7681 tgcggcccca tttgtctacc gttacaccgt gcgactccag ccacggcatc ccgtgtggat
     7741 tgaagccaaa cgccataatc accgcatcgg ctggcatgac aaattcactg ccctcaatcg
     7801 gtacaggtcg ccgacgcccc tgggcatccg gctcgccaag acgcgtgcgc aggaaacgaa
     7861 ttccgcaaac gtgaccttgt tcattcagct caagcgccac cggctggacg ttaaattcga
     7921 agttggcccc ctcttcgcgg gcgttcttca cctctttctt cgagcctggc atgttagctt
     7981 catcacgacg ataagcgcag gtgacgttac tcgcgccgtg gcgcagcgca gtacgcacac
     8041 agtccatcgc ggtgtcgccg ccgcccagta ccacgacgtt aagtccggcg gtattgataa
     8101 acggctcttc cggtagctct tcgagcccca tcacctgttt agtgttggca atgaggaacg
     8161 gcagcgcgtc ataaacgccc ggcgcatctt cattgggtaa acccgctttc atggaacggt
     8221 aagtgcctac gccaacgaag accgcgtcgt attgttccaa aagcgaatcc aaagagacat
     8281 ctttacccac ttcacaattg agttcgaagt gaatccccat cgcgctgaag atttcccgac
     8341 ggcgtgccag cagggattta tccagtttga aagaaggaat gccgaaagtg agcaagccac
     8401 caatttctgg atggcgatcg tacaccgtca ccgccacgcc attgcgggtc agaacatccg
     8461 cacaggccag ccctgccggg cctgccccga taatcgccac ccgcttgtcc actttggtga
     8521 catgacttaa gtcaggacgc caacctttcg ccaacgcctg atctgaaata tagcgttcaa
     8581 tgttgccgat agttacagca ccgtgctcat cgcgaatagt acaggcacct tcacacaaac
     8641 ggtcttgcgg acaaacgcgt ccggtaattt ccggcagggt gttggtctgg tgagaaagct
     8701 cgacggcggc gtcgatgttt ccggctttca ccagttcaat ccactgcgga atatggttat
     8761 gcagcgggca ggtccattca caaacgctgt gctcgccgca cttaaggcag cgcgaggctt
     8821 cccgttgtgc ctggtcggcg cgaaatggca gataaatttc atcaaaaccg gttttgcgcg
     8881 cttcaatcgc cagtttatcc ggctcgccac gcgcgggcgt tgcctgcatt tgttcgactt
     8941 tactcattac cggcatttct tgcgccgcgg tactggcatg ccacggttga tgttcctgac
     9001 gcgcggtgcg caagcggcgg gatttcgcca tgccggagag tgcgacgtca gtgaccagtt
     9061 gcagcgcgtc cgccgggcaa ttctcaacac aggcaggacc gttttcgcgc cccgcacaaa
     9121 ggtcgcattt atgcgccgtg gcttttactt ttcctgccgc gacgggtgtc aggacgattt
     9181 gcatcgtacc aaaaggacag gccaccacgc aggatttaca gccaatacac ttttgctgat
     9241 tgacctgaat gttgtcatca acgtggctga ttgcgccatt agggcagcta cgggcgcagg
     9301 gcgcatcttc acaatggtga caggtcactg cactacgttg ctgttgatgt ttgataaccg
     9361 taattcgggg atgaaaatgg tgttggctca ggacatgttg ctcatcattg tgagccatga
     9421 cacaggcgat ttcacaagca tgacaaccca gacattgctg actgttggcc ataataaaac
     9481 gattcataac gaccttcttt tttggttgta aaaaccttat tctttatatg agtgttgtta
     9541 ttacccgact tacaggggaa tcggcaatgt tcatgtgccc agaataagta actatttcgc
     9601 tataaactgt ggcagatcaa ataatcccat cactgactaa attgtgtttc agctaactgg
     9661 aacattaatg attttttgtg gagaagacgc gtgtgattgt taaacgaccc gtctcggcca
     9721 gtctggcccg ggcctttttt tacattgtgc tgctgtcgat tctttccacg ggtatcgctc
     9781 tgctaactct ggcgagcagt ttgcgcgacg ctgaggctat caatattgcc ggatcgctac
     9841 gtatgcagag ttaccgcctg ggctacgact tgcaaagtgg cagtccacaa ctcaatgcac
     9901 atcgccagtt gtttcagcag gcactgcatt caccggtatt aaccaatctc aacgtctggt
     9961 atgtgccaga agcagtaaaa actcgctatg cgcatctgaa tgccaactgg ctggagatga
    10021 ataatcggct cagcaagggc gatttgccgt ggtatcaggc caatattaat aattatgtta
    10081 atcagataga cctgttcgtg ctggctttac agcactacgc tgaacgcaaa atgctgctgg
    10141 tggtggcgat ttccctggct ggcggtatcg gtattttcac gctggtcttt tttactttgc
    10201 gccgtatacg ccatcaggtg gttgccccgc tgaatcagct ggttaccgcc agtcaacgta
    10261 ttgaacacgg gcagttcgac tcgccgccgc tggataccag tctgccgaat gagcttggtc
    10321 tgcttgcaaa aacctttaac cagatgtcga gcgagctgca taaattgtac ctttcgctgg
    10381 aggcgtcagt agaagaaaag acccgcgatc tccacgaggc caagcgtcgt ctggaggtgt
    10441 tgtatcaatg ttcccaggcg ctgaacacca gccagattga tgtgcattgt ttccgccata
    10501 ttttgcagat tgttcgcgat aatgaagcgg ctgaatatct ggagttaaat gtcggtgaaa
    10561 actggcggat tagcgaaggg caaccaaacc cggaattgcc gatgcagatt ttaccggtga
    10621 caatgcaaga gacggtttac ggcgaactgc actggcaaaa tagtcacgtt tcatcatcag
    10681 aaccgctgct taacagcgtt tcgtcgatgc tgggacgcgg tttgtacttt aatcaggcgc
    10741 agaagcattt tcagcagtta ttgttgatgg aagaacgtgc aaccatcgcc cgcgaattgc
    10801 acgactcgct ggctcaggta ctttcttact tacgtatcca gttgacgtta ctgaagcgtt
    10861 cgataccgga agataacgcc accgcacaaa gtatcatggc cgatttttcc caggcgttga
    10921 atgatgctta tcggcagtta cgcgagctgt tgactacttt ccgcctgacg ctgcagcagg
    10981 cggatctccc ctccgcgttg agggaaatgc tggatacgtt acaaaatcaa accagcgcca
    11041 aactgaccct cgactgccgt ctgccaaccc tggcgctgga tgcgcaaatg caggtgcatt
    11101 tgttgcaaat tattcgcgaa gcggtgctga atgcgatgaa gcacgccaac gccagcgaaa
    11161 tcgccgtcag ttgcgtcacc gcgccggacg gcaatcacac ggtttatatc cgtgataacg
    11221 ggattggtat cggtgaaccg aaagaacccg aaggtcatta tggtctgaat atcatgcgcg
    11281 aacgcgcgga acggctgggt gggacgctga ctttttcaca accttccggc ggcggcacgt
    11341 tagtgagtat tagctttcgc tccgcggagg gtgaggaaag tcagctgatg taatgcctcc
    11401 tactgaccaa agaatacttg cacttaaggt tcagtataaa agggcatgat aatttacatt
    11461 aactcctttt tttctccacg attggctcgt accttgccgc tacagtgaag caagtcaagc
    11521 ctacaacgat acgcagaaac acgaggtcct cttttaatgg cgaatttctt tattgatcgc
    11581 cccatttttg cctgggtgct ggcaatcctg ttgtgtctga caggtaccct ggcgattttc
    11641 tcattgcccg ttgaacaata ccccgatctc gcgccaccga atgtgcgagt gaccgctaac
    11701 tatcccggcg catcggccca gacgctggaa aacaccgtga cccaggttat cgagcaaaat
    11761 atgaccggcc tcgataatct catgtatatg tcatctcaga gcagtggcac cggtcaggca
    11821 tctgtcactt taagttttaa agcaggcacc gatccggacg aagccgtgca gcaagtacaa
    11881 aaccagctgc aatcagccat gcgaaagtta ccgcaggcgg tgcaaaatca gggcgtgacg
    11941 gtgcgtaaaa ccggcgatac caatattctg accattgctt tcgtctctac cgatggttcg
    12001 atggataaac aggatattgc cgattatgtt gccagtaata ttcaggaccc gttaagccgc
    12061 gtgaatggcg tcggggatat cgatgcctat ggttcgcaat attccatgcg tatctggctg
    12121 gacccggcga aactcaacag tttccagatg acggctaaag atgtcactga tgccattgag
    12181 tcacagaacg cgcagattgc ggttgggcaa cttggtggta caccttccgt cgataagcag
    12241 gcgctcaacg ccaccattaa cgcccagtca ctgctgcaaa caccagaaca gttccgcgat
    12301 atcaccttgc gggtgaatca agacggctca gaggtaaggc tgggcgatgt cgccaccgtc
    12361 gaaatggggg cggagaaata cgattatctt agccgcttca atggtaagcc agcctccggg
    12421 ctgggggtaa aactggcctc cggcgcaaac gaaatggcga cagcggagct ggtgctcaat
    12481 cgtctcgacg agctggcgca gtacttcccg cacggactgg aatacaaggt ggcgtatgaa
    12541 accacctcgt ttgttaaagc ctccattgaa gacgtggtga aaacgctgct ggaagctatc
    12601 gctctggttt tcctcgttat gtatctgttc ctgcaaaact tccgcgccac gctgataccc
    12661 actatcgccg tgccggtggt gttgatggga accttctccg tactttacgc cttcggttac
    12721 agcgtcaaca ccttaaccat gttcgcgatg gtgctggcga tcggtctgct ggtggatgac
    12781 gccatcgtgg tggtggaaaa cgtcgaacgt attatgagtg aggaaggact cactcctcgc
    12841 gaagccacac gtaaatcgat ggggcagatc cagggggcac tggtcgggat tgcgatggtt
    12901 ctttcggcgg tatttgtacc aatggccttc ttcggcggca ccaccggtgc catctatcgc
    12961 cagttctcta ttaccattgt tgcggcgatg gtgctgtcag tactggtagc gatgatcctc
    13021 actccggctc tgtgtgccac actacttaag ccactgaaaa aaggtgagca tcacgggcaa
    13081 aaaggctttt ttgcctggtt taaccagatg tttaaccgca acgccgaacg ctacgaaaaa
    13141 ggggtggcga aaattctcca ccgtagcctg cgctggattg tgatttatgt cctgctgctt
    13201 ggcggcatgg tgttcctgtt cctgcgtttg ccgacgtcgt tcttaccgct ggaagaccgt
    13261 ggcatgttta ctacctcggt acaattgccc agcggttcaa cccaacaaca gaccctgaaa
    13321 gtcgttgagc aaatcgagaa atattacttc acccatgaaa aagacaacat catgtcggtg
    13381 tttgccactg ttggttctgg ccccgggggt aacgggcaaa acgtggcgcg aatgtttatc
    13441 cgcctgaaag actggagcga acgcgacagt aagaccggca cctcgtttgc cattatcgag
    13501 cgtgcaacaa aggcgtttaa caagattaaa gaagctcgcg ttatcgccag cagcccgcca
    13561 gcaattagcg gtcttggtag ttctgcgggt tttgatatgg agttgcagga ccacgctgga
    13621 gcaggtcacg atgcgctgat ggcagcacgt aaccagttgc tggcgctggc ggcggaaaac
    13681 ccggagctaa cccgtgtgcg ccataacggc ctcgacgaca gtccgcagtt gcagattgat
    13741 atcgaccagc gtaaagctca ggcgctgggc gttgatatcg acgatattaa cgacacactg
    13801 caaaccgcct ggggttcgag ctatgttaat gactttatgg atcgcggtcg cgtgaagaaa
    13861 gtctatgtgc aggcagctgc gccgtatcgc atgctgccag atgacatcaa tctctggtat
    13921 gtccgaaata aagatggcgg catggtgccc ttctctgctt tcgcgacctc acgctgggag
    13981 acaggctcgc cgcgtctgga acgctataac ggttattctg cggttgagat tgttggggaa
    14041 gccgcaccgg gggtcagtac cggtacggcg atggatatta tggaatcgtt agtgaagcag
    14101 ttgccaaacg gctttggtct ggagtggacg gcgatgtcgt atcaggagcg gctttccggc
    14161 gcgcaggctc cggcgctgta cgctatttcc ttgctggtgg tattcctgtg tctggcggcg
    14221 ttgtatgaaa gctggtcggt gccgttctcg gtaatgctgg tcgtgccgct gggggttatc
    14281 ggcgcgctgc tggcaacctg gatgcgcggg ctggaaaacg acgtttactt ccaggtgggc
    14341 ctgttaacgg tcattggttt atcggcgaaa aacgccatcc tgatcgtcga gtttgctaac
    14401 gagatgaacc aaaaaggcca cgacctgttt gaagcgacgc tccacgcctg ccgtcagcgt
    14461 ttacgcccga ttctgatgac ctcgctggca tttatcttcg gcgtattgcc aatggcaacc
    14521 agcacgggtg ccggttccgg tggtcagcat gcggtgggta ctggcgtaat gggcgggatg
    14581 atttcggcca ctattctggc tatttacttc gtgccgctgt tctttgtact ggtgcgccgc
    14641 cgcttcccgc tgaagccgcg cccggaataa gcaataaaaa aggcgacatg ctaatgtgtc
    14701 gcctttttca actttccgat taagaacctg ctcagcgggt tcttgctgtt tgtactttgt
    14761 ctcaggaatt acttacgaag cataacttcg ataaagtctt tccagttccc cagttcacgt
    14821 tcaatcataa caacctctct tataattatg ggtattctac ggaaacaata taccgtggtg
    14881 aagctaattt actcgattgc tgcgatgact acctccgggg gacaaacctt atgtaaatac
    14941 tatggtcctg cggtgataat ttgtatgtga tacacagcaa catttcgaga t
//
LOCUS       DBANUU010000105        14975 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000105 DBANUU010000000
VERSION     DBANUU010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.105"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..76
                     /locus_tag="ACIE68_003372"
     CDS             <1..76
                     /locus_tag="ACIE68_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFX5909636.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            102..344
                     /gene="yjbE"
                     /locus_tag="ACIE68_003373"
     CDS             102..344
                     /gene="yjbE"
                     /locus_tag="ACIE68_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFX5909637.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            458..1096
                     /gene="yjbF"
                     /locus_tag="ACIE68_003374"
     CDS             458..1096
                     /gene="yjbF"
                     /locus_tag="ACIE68_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFX5909638.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1093..1830
                     /gene="yjbG"
                     /locus_tag="ACIE68_003375"
     CDS             1093..1830
                     /gene="yjbG"
                     /locus_tag="ACIE68_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFX5909639.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1830..3926
                     /gene="yjbH"
                     /locus_tag="ACIE68_003376"
     CDS             1830..3926
                     /gene="yjbH"
                     /locus_tag="ACIE68_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFX5909640.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3973..4251)
                     /locus_tag="ACIE68_003377"
     CDS             complement(3973..4251)
                     /locus_tag="ACIE68_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909641.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4252..4395
                     /locus_tag="ACIE68_003378"
     CDS             4252..4395
                     /locus_tag="ACIE68_003378"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909642.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4521..4931
                     /gene="psiE"
                     /locus_tag="ACIE68_003379"
     CDS             4521..4931
                     /gene="psiE"
                     /locus_tag="ACIE68_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFX5909643.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4975..6450)
                     /gene="xylE"
                     /locus_tag="ACIE68_003380"
     CDS             complement(4975..6450)
                     /gene="xylE"
                     /locus_tag="ACIE68_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFX5909644.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSSRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSILQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6822..7712)
                     /gene="malG"
                     /locus_tag="ACIE68_003381"
     CDS             complement(6822..7712)
                     /gene="malG"
                     /locus_tag="ACIE68_003381"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFX5909645.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7727..9271)
                     /gene="malF"
                     /locus_tag="ACIE68_003382"
     CDS             complement(7727..9271)
                     /gene="malF"
                     /locus_tag="ACIE68_003382"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFX5909646.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNKFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9425..10615)
                     /gene="malE"
                     /locus_tag="ACIE68_003383"
     CDS             complement(9425..10615)
                     /gene="malE"
                     /locus_tag="ACIE68_003383"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFX5909647.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10980..12095
                     /gene="malK"
                     /locus_tag="ACIE68_003384"
     CDS             10980..12095
                     /gene="malK"
                     /locus_tag="ACIE68_003384"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFX5909648.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12167..13507
                     /gene="lamB"
                     /locus_tag="ACIE68_003385"
     CDS             12167..13507
                     /gene="lamB"
                     /locus_tag="ACIE68_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFX5909649.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13750..14670
                     /gene="malM"
                     /locus_tag="ACIE68_003386"
     CDS             13750..14670
                     /gene="malM"
                     /locus_tag="ACIE68_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFX5909650.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         3704 a         3850 c         3957 g         3464 t
ORIGIN      
        1 gctgtcgttc tcaaaatcgg tggagctgca tgacaaagtc atcgggcatt atctgaacat
       61 aaaacactat caataagttg gagtcattac cggaaattgt tatgaaaaaa gttctgtatg
      121 gcatttttgc catatctgcg cttgcggcga cttctgcgtg ggctgcacct gtacaggtgg
      181 gcgaagcggc agggtcggca gcaacgtcgg tttcggcggg gagttcctcc gcgaccagcg
      241 tcagcaccgt aagctcggcg gtgggtgtcg cgctcgcggc aaccggtggc ggtgatggtt
      301 ctaacaccgg gaccaccaca accacgacca ccagtaccca gtaataaagt atgtatcccc
      361 aaaataattt gagtcattgc atctgtggct tgaagtatga agggatttac cataaccaca
      421 ctccggtgtg gttattctgc ccctctggag aagagtcgtg aagcgacctg cactcattct
      481 tatctgcctg ctattacagg cctgttcagc cacgactaaa gagctgggca attcactgtg
      541 ggacagtctg ttcggcacgc caggcgtaca gctgacggac gatgatattc aaaatatgcc
      601 ctacgccagc cagtacatgc agcttaatgg cgggccgcaa ttgtttgtgg tgctggcctt
      661 cgctgaagac ggacaacaaa aatgggtcac tcaggatcaa gccactcttg tcacacaaca
      721 tggtcgtctg gtgaagactt tgcttggcgg cgacaacctg attgaagtga ataaccttgc
      781 cgccgacccg ctgattaaac ccgcacaaat tgttgatggt gcaacatgga cgcgcacgat
      841 gggctggacc gagtaccagc aggtacgcta cgccaccgca cgctcagtct tcaaatggga
      901 tggcaccgat accgtcaagg tcggcagcga tgaaaccccg gttcgcgtgc tggacgaaga
      961 agtctccacc gaccaggcgc gctggcataa ccgctactgg atcgacagcg aagggcaaat
     1021 tcgccagtcg gaacagtatc tcggcgcgga ttatttcccg gtgaaaacca ctctcatcaa
     1081 ggcggcaaaa caatgattaa acaaactatt gtcgcattga ttttgagtgt gggggcgtca
     1141 tcggtcttcg cggcgggaac cgtcaaggta ttcagcaatg gcagcagtga ggccaaaacg
     1201 ctgacgggcg cagagtattt aatcgatctg gtaggccagc cgcggctggc gaatagctgg
     1261 tggcctggtg cggtgattag cgaagagctg gcaacggcgg cagcattgcg gcagcagcag
     1321 gcgttgctga cacggctggc agaactggct gcagattcca gcgccgacga tgccgccgcg
     1381 attaacgcct tacgccagca aattcaggcg ttgaaagtga cgggcaggca aaaaatcaat
     1441 cttgatcctg atatcgtccg cgttgccgaa cgcggtaacc caccgttgca gggcaactac
     1501 acgctgtggg tcgggccacc accgtccacg gtcacgctgt tcgggcttat cagccgtcct
     1561 ggcaagcagc cattcactcc aggtcgcgac gtggcgagct atctctctga ccagagcctg
     1621 ctcagcggtg cggatcgcag ctacgcgtgg gtcgtttacc cggacggacg cacacaaaaa
     1681 gcgccggtgg cttactggaa caagcgtcac gtagagccga tgcccggcag cattatttat
     1741 gttggcctcg cggactccgt ctggagtgag acgcctgatg cccttaacgc cgacattctt
     1801 cagactctga cgcagcggat accccaataa tgaaaaaaag acatctgctt agcttactgg
     1861 cgctgggcat tagcacagct tgctacggcg aaacatatcc tgcgcccatt ggtccgtcgc
     1921 agtcggattt cggtggcgta ggattattac aaacgcccac cgcgcgcatg gcgcgggaag
     1981 gggagttgag cctgaactat cgagataacg atcagtaccg ttattactca gcttcagtgc
     2041 aactcttccc gtggctggaa acaacgctgc gctacaccga cgtgcgcacc cggcagtaca
     2101 gcagcgtcga agcgttctct ggcgatcaaa cgtataaaga taaagccttc gatctcaaac
     2161 tgcgtttgtg ggaagagagt tactggctgc cgcaagtggc ggttggtgcg cgggatatcg
     2221 gcggtacggg gctgtttgat gcggaatatc ttgttgccag caaagcctgg gggccgttcg
     2281 attttacgct cggcctgggc tggggatatc tgggcaccag cggtaatgtg aaaaatccgc
     2341 tctgttcagc cagtgataaa tattgctatc gcgataacag ctacaaacag gcgggatcta
     2401 tcgacggtag ccagatgttc cacggtcctg cctcactgtt tggcggcgtg gaataccaga
     2461 cgccctggca accgctgcgc ctgaaactgg agtatgaagg caataattat cagcaggatt
     2521 ttgccgggaa gctggagcaa aaaagtaagt ttaacgtcgg tgcgatttat cgcgttaccg
     2581 attgggccga cgttaacctt agctatgaac gtggcaacac ctttatgttt ggcgttacgt
     2641 tgcgcaccaa ctttaacgat ctgcgcccgt cttacaacga taacgcccgc ccgcaatatc
     2701 aaccgcagcc gcaggatgcc attttgcagc attcggtggt ggcgaatcag ttaacgctgt
     2761 tgaaatacaa tgccggactt gccgatccac agatccaggc gaaaggcgat acgctgtatg
     2821 ttaccggcga gcaggtgaaa tatcgtgatt cgcgcgaagg gatcatccgt gctaatcgga
     2881 tcgtgatgaa cgatctgccg gatgggatca aaacgatccg cattacggaa aatcgcctta
     2941 acatgccgca ggtgacgacg gaaacggatg tcgccagcct gaaaaatcat ctcgccggag
     3001 agccgttggg ccacgaaacg aagctggcgc aaaaacgcgt cgagccagtg gttccgaagt
     3061 ccaccgagca gggctggtat atcgacaaat cgcgctttga tttccatatc gatccggtgc
     3121 tgaaccagtc ggtcggtggc ccggaaaact tttacatgta tcagctgggc gtgatgggaa
     3181 cggcagattt gtggctgacg gaccatctgc tgaccaccgg cagcctgttt gcaaatcttg
     3241 ccaacaacta cgacaagttt aactacacca atccgccgca ggactcgcac ttaccgcgcg
     3301 tgcgtaccca tgtgcgcgag tatgtgcaga acgatgtcta tgtgaataac ctgcaagcca
     3361 actacttcca gcatctgggc aacggcttct acggtcaggt ctacggcggc tatctcgaaa
     3421 ccatgtttgg cggtgcgggg gctgaggtgt tgtatcgccc gctggacagc aactgggcgt
     3481 ttggtctgga tgccaactac gttaaacagc gcgactggcg tagtgcaaaa gatatgatga
     3541 aattcaccga ctacagcgtg aaaaccggac atctgaccgc ctactggacg ccatctttcg
     3601 ctcaggatgt gttagttaaa gccagcgtcg ggcagtatct ggcaggggat aaaggcggca
     3661 cgctggagat cgccaaacgc tttgatagcg gtgtggtggt gggtggctat gccacgatca
     3721 cgaatgtttc gaaagaggag tacggcgaag gggacttcac caaaggcgtg tatgtctcgg
     3781 taccgttgga tctcttctcg tctggcccga cacgcagccg tgcggcaatt ggctggacgc
     3841 cgctgacgcg tgacggtggt cagcaacttg ggcgtaagtt ccagttgtat gacatgacca
     3901 gcgaccgtag cgtaaatttc cgctaagtca tgggaaaggt gccagttttc gcactcactg
     3961 gcaccttcat tcttaatggc ctgtcgagcc atcaggaacg cagttcaccc ccggtttgcg
     4021 atctggtgtg taaaggcgaa aacattctgt cttcctgtcc gtcgatattg gttgcaggtc
     4081 tggttgcctg agcgactggg ccatcattgg gctggagaca ttgctttgcg gtaggtcggc
     4141 tttatcagca gtaccgagcg gaccagcata agcaggaaga acagagactg ataacatcaa
     4201 agcagcaaaa taaggcttca tttttaccac ctttatcagg ttacgtttca tttgttccag
     4261 aggaacattg tcgatttttc gcgcattgct ggtggctggg aatcacctga atgggtgatt
     4321 tttgaattac cggctttggt gcggtttgtc ttgccggatg cgccgccagg cgcggcttat
     4381 ccggcctacg ggtaggtata tccggctttg gtggaggcgc gctccaaatc caggttgaac
     4441 aaaacataca caaaaaatat agatctccgt cacatttttg cgttatacag gaagctcgcc
     4501 actgtgaagg aggtactgct atgacgtcac tctctcgtcc gcgcgtggag tttatctcca
     4561 ccattttgca gaccgtactc aatcttggcc tgttgtgcct cggcctgatt ttggttgtct
     4621 tcctcggcaa agaaacggtg catctggctg atgtgctatt cgcgccagaa caaaccagca
     4681 aatatgagct ggtagaagga ctggtggttt actttctcta tttcgaattt atcgcgctga
     4741 ttgtgaagta ctttcagtcc ggttttcact tcccgctacg ttactttgtc tatattggga
     4801 tcaccgccat tgtgcgcttg atcatcgtcg atcataaatc gccactggat gtgttgatct
     4861 actcggctgc gatcctgtta ctggtgatca ccctgtggct gtgtaattcg aaacggctga
     4921 agcgggagta aaaagacagc acgccgaact ggcgcggcgt gctggacagg aagattacag
     4981 cgtagcagtt tgttgtgttt tcttcgtttc cggttcccag agggcttcca gctcctcaag
     5041 ggttttacct ttggtttccg ggacaaattt ccacataaat agtgctgcca gaacgcccat
     5101 acacccgtaa atccagtagg agaaaccgtt gtggaaatgg gccaccagcc aggagttttt
     5161 gtccatcatc gggaaggtcc aggagacgaa gtagttcgcc agccactggg ccgccaccgc
     5221 gattgccagc gctttaccac gaatagcatt cgggaagatt tccgacagca gtacccagca
     5281 taccggaccc caggacatgg caaaggcggc aacatagaac agcatcgaca gaagcgccac
     5341 aatacccggt gcctgagtgt aaaacgcggt accgaggcta aacataccga ttgccattcc
     5401 gagtgcgccg ataatttgca gtggcttacg accaaattta tccaccgtca taattgccag
     5461 aacggtgaag gtgaggttga taactccgac aataatggtc tgcaacagcg cgatatccgt
     5521 gctggccccc agcgttttga acacttccgg cgcgtagtac agcaccacat tgatgccgac
     5581 aaattgctgg aggatggaga gcataacgcc gatgacaatc acgcctacgc caaacatcag
     5641 cagacgacca ccggttttgc ggccatgatc cagagagtgt ttaatttcct gtactgcctg
     5701 agttgcaagg gtgttgccca taattttgcg caggatacct tccgcctgtt cttgcttgcc
     5761 gcgtgacatc agccagcgag gactttctgg cacggtatac agcagcatta agaacagcag
     5821 tgcagggata cattccgagg caaacatata acgccagccg tcagtattca gccagctggc
     5881 gtcgccggaa cgggcaataa aatagtttac gcagtaaact aaaagctgcc cgaaaataat
     5941 cgcaaactgg ttaaaagaga ccagtttccc gcgaatatga gcaggagcaa gttccgcaat
     6001 atacattggt gaaagcattg aggctaaacc aacgccaata ccgccaataa tgcgataaat
     6061 aacaaattcc gggacataac ctgccagata aacaggcact gtgttgtccg ggtttataga
     6121 ggtaaaacca agttctggcc aggcagaacc tacaccagaa ataaaaaaca ggacagcagc
     6181 aatcttaagt gaatcacgac gaccgaagcg gctactgcaa taaccaccga gggcaccgcc
     6241 gatgatgcaa ccaatcagag cgctggccac gcaaaaccct aacagggagt tggcagcgga
     6301 ttcacttaag ttttgtggag caacaaagac ggtattgagt gactcaacag taccggaaat
     6361 aacggcggtg tcgtagccaa ataataaacc acctaatgta gcgactaagg taatcgaaaa
     6421 tatataactg gaattatact gggtattcat tcagacctgc cttagaccat tctgatcttt
     6481 tatcagtgca ttgatgttgg aattgtcagg cagttaaggc aattatcatt ttttacaatg
     6541 cgtttacgta atgtcttttc tgtgatctta attgtgataa ttatcaaaaa ttgatgaaaa
     6601 caatactatt gccgtgactc agagcacgaa agagaattat cgtaagtggg aaaacaaata
     6661 acgtaaaaat aataagttct atgatggaaa tattaaccgg cgaacgattc agattgcaga
     6721 cgaaagaaaa aaaggcgctc cgtggagcgc cgaataacag tcacaagttg ggataacgta
     6781 agttgagggt gcagcggcat aacattggca gaacaacatc tttaaccttt cacaccacct
     6841 gccgtcaggc cgttgaccag ccagcgttga gccagcaaga agacgatggt gatcggtaat
     6901 gcagacatca cggcagcggc ggcaaagtca ccccacaggt agttttgcgg gttgaggtat
     6961 tgctgcatcc ccacggccag ggtgtagctg tttacgtcac gcagtaacag cgacgcgacc
     7021 ggaacttcag taatggcagc gataaacgac aggatgaaca ccaccgccag aatcggtact
     7081 gacagcggca acaggacaag gcggaaggcc tgccacggtg tcgcaccatc cagcgccgcc
     7141 gcttcttcca gcgaactgtc gatggtttcg aaatagcctt tgatggtcca gacgtgcagc
     7201 gcaatcccac ccagatacgc gaaaattacg ccgccgtgag tattcaggcc aatgaatgga
     7261 atgtactcac ccagacgatc aaacaacgca tacaacgcga ccagtgaaag tactgccggg
     7321 aacatctgga aaatcagcat tcctttcagc agcgtcgctt tgcctggaaa gcgcatacgg
     7381 gcgaaagcgt aggcgcaggt ggtggagagc gccacaatgc caatcgcgga aatcccggcg
     7441 acctttaccg agttccacag ccacagcagt accgggaatg gcggtggcgt aatgcgacca
     7501 tcagcctgtt ccacgctaaa acctaatgcc agtttccagt gatcccagga gatttgctcc
     7561 gggatcaggc taccggtcgc aaagttcccc tggcgcagtg agatggcgac gaccatcagc
     7621 agcgggaaca taatcgccgc gataaaaagt agcagtagca ggtgagtaat aaacaaacgt
     7681 gctttttgcg atttcggttg gaccattgcc atttttgtta tctcccttaa tcaaacttca
     7741 ttcgcgtggc tttcaggttc actatcgcca gcgcacccac cagcaggaag atcagcgtgg
     7801 ctattgctgc tgccagaccg aagtcctgac ccccgccgcc ttcaaaagcg atgcggtagg
     7861 tgtagttaac aagcaggtcg gtataaccgg ctggcgtggt cgtgccaaga cgatccgggc
     7921 cgccgttggt taacagttga atcagcacga atttgttaaa gttaaaggcg aagctggcga
     7981 tcatcagcgg cgtcagcggt ttaatcagca gcggcagcgt aatcttaaag aagttctgga
     8041 agggacctgc accatccatt gctgaggctt catacaaatc gtccggaatc gctttcagca
     8101 aacccatgca gaggatcatc atgtacggat aacccagcca ggtattgacg ataatcagca
     8161 tcgttcgggc ggtggtcgga tcgctgaacc aggcgggctt cacgccaaac agcgcgctca
     8221 acatcatgtt gatctcaccg aagctctggt taaacaaccc tttgaaaatc aagattgaaa
     8281 tgaatgatgg caccgcgtag ggcagaatca gcaggacgcg atagaccgct ttgccgcgca
     8341 acgcttccca ctgcaccaga cacgccagaa ccatgccgac cgccaccgtt aaaaagacag
     8401 tgatcagcga gaacaccacg gtccagacga aaatggcgag gaacggtttc tgaatgcctt
     8461 cgtcggtgaa gacgcgggta aagtttttcc agccagtagt cacggtgtaa ccggggctta
     8521 gcttttcatc gccccagttg ccgtcggcgg taatggactg gtaaaagcca atttggttat
     8581 tcggacgata tttcacgccg ctctgattat tcgtcaacgt gccgtcaccg tcgagtgtgt
     8641 agagcggctg cgtgccagaa aactggcgca gggagctcat catcactttg ttgccatccg
     8701 gcagaatggc ggtaatgtca ctcagcgcct gacgattctg ggtaatcacg cgcagatttg
     8761 cgcgttcgcc ttcgggctgg gcggtcgttt ctttcagttg cagtttttgc tcgccgccaa
     8821 atttaaaagc gtcggagagg taatttttgc cggtttcgcc gtcgctgagg gctagttgcc
     8881 actcatcgcc cgccgggtaa agaccaaaat tataggtttt gcctgcttgc caggagcgat
     8941 ctaacaacac ttcctgcgca cgttcaaaag tcagctggtt agtgctgctg tagttggtga
     9001 aggcaatggc gatggtgcag accagaggga agaggacgaa taatcccatt ccggccattc
     9061 ccgggtaaac atagcgccag gcgtaggctt tacgattggc gaaaatatac agccccgctg
     9121 aactcaatat cagcgtggta atggcaaaca ggtattcccc ttgtgcgtac attaaaacaa
     9181 caaggtaacc caccagcagg ccgagcagac ctagcactga ccatttcagc gcgtcgcttt
     9241 gccaccaatg tttcttttta atgacatcca tggggttctt cctcattcca ggacggataa
     9301 ggctttcacg ccttatccga caacaactgc ctgatgcgac actgatgcgt cttatcaggc
     9361 ctacatacgt ttcggttttg taggccggac aaggcgttca cgccgcatcc ggcatttcac
     9421 agcattactt ggtgatacga gtctgcgcgt ctttcagggc ttcatcgaca gtctgacgac
     9481 cgctggcggc gttgatcacc gcagtacgca cggcatacca gaaagcggac atctgcggga
     9541 tgttcggcat gatttcacct ttctgggcgt tttccatggt ggctgcaata cgtggatctt
     9601 tcgccaactc ttcctcgtaa gacttcagcg ctacggcacc cagcggtttg tctttattaa
     9661 ccgcttccag accttcgtca gtcagcagat agttttcgag gaactctttc gccagctctt
     9721 tgttcggact ggcggcgtta ataccggcgc tcagcacgcc aacgaacggt ttagatggtt
     9781 gacccttgaa ggtcggcagt accgttacac cataattcac tttgctggtg tcgatgttgg
     9841 accatgccca cgggccgttg atggtcatcg ctgtttcgcc tttattaaag gcagcttctg
     9901 cgatggagta atcggtgtct gcattcatgt gtttgttttt aatcaggtca accaggaagg
     9961 tcagacccgc tttcgcgcca gcgttatcca cgcccacgtc tttaatgtcg tatttgccat
    10021 tttcatactt gaacgcataa cccccgtcag cagcaatcag cggccaggtg aagtacggtt
    10081 cttgcaggtt gaacatcagc gcgctcttac ctttcgcttt cagttcttta tccagcgccg
    10141 ggatctcttc ccaggttttt ggtgggttcg gcagcagatc tttgttataa atcagcgata
    10201 acgcttcaac agcgatcggg taagcaatca gcttgccgtt gtaacgtacg gcatcccagg
    10261 taaacggata cagcttgtcc tggaacgctt tgtccggggt gatttcagcc aacaggccag
    10321 attgagcgta gccaccaaaa cggtcgtgcg cccagaagat aatgtcaggg ccatcgccag
    10381 ttgccgcaac ctgagggaat ttctcttcca gtttatccgg atgctcaacg gtgactttaa
    10441 ttccggtatc tttctcgaat ttcttaccga cttcagcgag accgttatag cctttatcgc
    10501 cgttaatcca gattaccagt ttaccttctt cgattttggc gagagccgag gcggaaaaca
    10561 tcatcgtcgt taatgcggat aatgcgagga tgcgtgcacc tgtttttatt ttcataatct
    10621 atggtccttg ttggtgaagt gctcgtgaaa acacctaaac ggactctagt ttctttatac
    10681 ggcaacctct ttccatcctc cttgccccta cgccccaccg ttgctttgtg tgatctctgt
    10741 tacagaattg gcggtaatgt ggagatgcgc acataaaatc gccacgattt ttgcaagcaa
    10801 catcacgaaa ttccttacat gacctcggtt tagttcacag aagccgtgtt ctcatcctcc
    10861 cgcctcctcc cccataaaaa agccaggggg tggaggattt aagccatctc ctgatgacgc
    10921 atagtcagcc catcatgaat gttgctgtcg atgacaggtt gttacaaagg gagaagggca
    10981 tggcgagcgt acagctgcaa aatgtaacga aagcctgggg cgaggtcgtg gtatcgaaag
    11041 atatcaatct cgatatccat gaaggtgaat tcgtggtgtt tgtcggaccg tctggctgcg
    11101 gtaaatcgac tttactgcgc atgattgccg ggcttgagac gatcaccagc ggcgacctgt
    11161 tcatcggtga gaaacggatg aatgacactc cgccagcaga acgcggcgtt ggtatggtgt
    11221 ttcagtctta cgcgctctat ccccacctgt cagtagcaga aaacatgtca tttggcctga
    11281 aactggctgg cgcaaaaaaa gaggtgatta accaacgcgt caaccaggtg gcggaagtgc
    11341 tgcaactggc gcatttgctg gatcgcaaac cgaaagcgct ctctggcggt cagcgtcagc
    11401 gtgtggcgat tggccgtacg ctggtggccg agccaagcgt atttttgctc gatgaaccgc
    11461 tctccaacct cgatgctgca ctgcgtgtgc aaatgcgtat cgaaatctcc cgtctgcata
    11521 aacgcctggg ccgcacaatg atttacgtca cccacgatca ggtcgaagcg atgacgctgg
    11581 ccgacaaaat cgtggtgctg gacgccggtc gcgtggcgca ggttgggaaa ccgctggagc
    11641 tgtaccacta tccggcagac cgttttgtcg ccggatttat cggttcgcca aagatgaact
    11701 tcctgccggt aaaagtgacc gccaccgcaa tcgatcaagt gcaggtggag ctgccgatgc
    11761 caaatcgtca gcaagtctgg ctgccagttg aaagccgtga tgtccaggtt ggagccaata
    11821 tgtcgctggg tattcgcccg gaacatctac tgccgagtga tatcgctgac gtcatccttg
    11881 agggtgaagt tcaggtcgtc gagcaactcg gcaacgaaac ccaaatccat atccagatcc
    11941 cttccattcg tcaaaacctg gtgtaccgcc agaacgacgt ggtgttggta gaagaaggtg
    12001 ccacattcgc tatcggcctg ccgccagagc gttgccatct gttccgtgag gatggcactg
    12061 catgtcgtcg actgcataag gagccgggcg tttaagcacc ccacaaaaca cacaaagcct
    12121 gtcacaggtg atgtgaaaaa agaaaagcaa tgactcagga gatagaatga tgattactct
    12181 gcgcaaactt cctctggcgg ttgccgtcgc agcgggcgta atgtctgctc aggcaatggc
    12241 tgttgatttc cacggctatg cacgttccgg tattggctgg acaggtagcg gcggtgaaca
    12301 acagtgtttc cagactaccg gtgctcaaag taaataccgt cttggcaacg aatgtgaaac
    12361 ttatgctgaa ttaaaattgg gtcaggaagt gtggaaagag ggcgataaga gcttctattt
    12421 cgacactaac gtggcctatt ccgtcgcgca acagaatgac tgggaagcta ccgatccggc
    12481 cttccgtgaa gcaaacgtgc agggtaaaaa cctgatcgaa tggctgccag gttccaccat
    12541 ctgggcaggt aagcgcttct accaacgtca tgacgttcat atgatcgact tctactactg
    12601 ggatatttct ggtcctggtg ccggtctgga aaacatcgat gttggcttcg gtaaactctc
    12661 tctggcagca acccgctcct ctgaagctgg tggttcttcc tcttttgcca gcaacaatat
    12721 ttatgactat accaacgaaa ccgcgaacga cgttttcgat gtgcgtttag cgcagatgga
    12781 aatcaacccg ggcggcacat tagaactggg tgtcgactac ggtcgtgcca acctgcgtga
    12841 taactatcgt ctggttgatg gcgcatcgaa agacggctgg ttattcactg ctgaacatac
    12901 tcagagtgtc ctgaagggct ttaacaagtt tgttgttcag tacgctactg actcgatgac
    12961 ctcgcagggt aaaggtctgt cgcagggttc tggcgtcgcg tttgataacg aaaaatttgc
    13021 ctacaatatc aacaacaacg gtcacatgct gcgtatcctc gaccacggtg cgatctccat
    13081 gggcgacaac tgggacatga tgtacgtggg tatgtaccag gatatcaact gggataacga
    13141 caacggcacc aagtggtgga ccgttggtat tcgcccgatg tacaagtgga cgccaatcat
    13201 gagcaccgtg atggaaatcg gctacgacaa cgtcgaatcc cagcgcaccg gcgacaagaa
    13261 caatcagtac aaaattaccc tcgcacaaca atggcaggct ggcgacagca tctggtcacg
    13321 cccggctatt cgtgtcttcg caacctacgc caagtgggat gagaaatggg gttatgacta
    13381 caacggcgat agcaaggtta acccgaacta cggcaaagcc gttcctgctg atttcaacgg
    13441 cggcagcttc ggtcgtggcg acagcgacga gtggaccttc ggtgcccaga tggaaatctg
    13501 gtggtaatag caaaacctgg gccggataag gcgtttacgc cgcattcgac aaccaacgcc
    13561 tgatgcgacg cttgcgcgtc ttatcaggcc tacaacggct gtcaaatgta ggccggataa
    13621 ggcgtttacg ccgcatccgg cataaaaaca ggttgtcatt atctgaaagg ggcgaaagcc
    13681 cctctgatta tcgggtttag cgcgctattg cctggctacc gctgagctcc agattttgag
    13741 gtgaaaacaa tgaaaatgaa taaaagtctc atcgccctct gtttatcagc agggttactg
    13801 gcaagcgcgc ctggaatcag ccttgccgat gttaactacg taccgcaaaa caccagcgac
    13861 gcgccagcca ttccatctgc tgcgctgcaa caactcacct ggacaccggt cgatcaatct
    13921 aaaacccaga ccacccaact ggcgaccggc ggccaacaac tgaacgttcc cggcatcagt
    13981 ggtccggttg ctgcgtacag cgtcccggca aacattggcg aactgaccct gacgctgacc
    14041 agcgaagtga acaaacaaac cagcgttttt gcgccgaacg tgctgattct tgatcagaac
    14101 atgaccccat cagccttctt ccccagcagt tatttcacct accaggaacc aggcgtgatg
    14161 agtgcagatc ggctggaagg cgttatgcgc ctgacaccgg cgttggggca gcaaaaactt
    14221 tatgttctgg tctttaccac ggaaaaagat ctccagcaga cgacccaact gctcgacccg
    14281 gctaaagcct atgccaaggg cgtcggtaac tcgatcccgg atatccccga tccggttgct
    14341 cgtcatacca ccgatggctt actgaaactg aaagtgaaaa cgaactccag ctccagcgtg
    14401 ttggtaggac ctttatttgg ttcttccgct ccagctccgg ttacggtagg taacacggcg
    14461 gcaccagctg tggctgcacc cgctccggca ccggtgaaga aaagcgagcc gatgctcaac
    14521 gacacggaaa gttattttaa taccgcgatc aaaaacgctg tcgcgaaagg tgatgttgat
    14581 aaggcgttaa aactgcttga tgaagctgaa cgcctgggat cgacatctgc ccgttccacc
    14641 tttatcagca gtgtaaaagg caaggggtaa ttacgcccca cagtgctgat tttgcaacaa
    14701 ctggtgcgtc tcctggcgca ccttttttta tgcttccttc ctgggatatg agcgattttt
    14761 tatagtaact cacttcttct tcactaagaa tatccattat ctcaatgcct tatcagagat
    14821 tcttttcctt tcgccggtag tgtctggaca ttcaggctac ttttccaggt tattttattt
    14881 ctgttatgca gaggttttat gataagtcat atcctaaatt ctggcggcaa taactctttg
    14941 atgaaacatg atgtggtgca aggaaataat atagt
//
LOCUS       DBANUU010000106        14931 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000106 DBANUU010000000
VERSION     DBANUU010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.106"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..739
                     /locus_tag="ACIE68_003387"
     CDS             <1..739
                     /locus_tag="ACIE68_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFX5909651.1"
                     /translation="FFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVNV
                     RLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASTS
                     LPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPLA
                     LKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTGL
                     NGKASSAKVHFYAINDFGGAIEGNASL"
     gene            774..3374
                     /gene="htrE"
                     /locus_tag="ACIE68_003388"
     CDS             774..3374
                     /gene="htrE"
                     /locus_tag="ACIE68_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFX5909652.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RLSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIE"
     gene            3391..3960
                     /locus_tag="ACIE68_003389"
     CDS             3391..3960
                     /locus_tag="ACIE68_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5909653.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            3975..4577
                     /gene="yadL"
                     /locus_tag="ACIE68_003390"
     CDS             3975..4577
                     /gene="yadL"
                     /locus_tag="ACIE68_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFX5909654.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            4604..5200
                     /locus_tag="ACIE68_003391"
     CDS             4604..5200
                     /locus_tag="ACIE68_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFX5909655.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            5184..6527
                     /locus_tag="ACIE68_003392"
     CDS             5184..6527
                     /locus_tag="ACIE68_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFX5909656.1"
                     /translation="MCTTNNRSKQITATVTAVDGGVIMKKFFRHFLFLILCLSCYTAS
                     AGTDDNVGYIVGNSYGVGPSDQKWRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTG
                     VKLQWSQLDTASGGGFLYCNRSDSTSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYY
                     TLLISNMWSAYGTVTNVSSPGIYIGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAV
                     GGIYQTLTIEFYTDTNFDPTVTQQIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFD
                     LLNVKLTNPTCFTAMLSGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNI
                     ETKLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKD
                     YDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            6640..7434
                     /gene="panB"
                     /locus_tag="ACIE68_003393"
     CDS             6640..7434
                     /gene="panB"
                     /locus_tag="ACIE68_003393"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFX5909657.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITDALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7446..8297
                     /gene="panC"
                     /locus_tag="ACIE68_003394"
     CDS             7446..8297
                     /gene="panC"
                     /locus_tag="ACIE68_003394"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFX5909658.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(8371..9273)
                     /gene="rpnC"
                     /locus_tag="ACIE68_003395"
     CDS             complement(8371..9273)
                     /gene="rpnC"
                     /locus_tag="ACIE68_003395"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFX5909659.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMTNLSLAEIDKVINLI"
     gene            9547..9927
                     /gene="panD"
                     /locus_tag="ACIE68_003396"
     CDS             9547..9927
                     /gene="panD"
                     /locus_tag="ACIE68_003396"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFX5909660.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(9931..11160)
                     /locus_tag="ACIE68_003397"
     CDS             complement(9931..11160)
                     /locus_tag="ACIE68_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFX5909661.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLMSNQPQG"
     gene            complement(11224..11664)
                     /locus_tag="ACIE68_003398"
     CDS             complement(11224..11664)
                     /locus_tag="ACIE68_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFX5909662.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(11769..12539)
                     /locus_tag="ACIE68_003399"
     CDS             complement(11769..12539)
                     /locus_tag="ACIE68_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFX5909663.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(12536..13462)
                     /locus_tag="ACIE68_003400"
     CDS             complement(12536..13462)
                     /locus_tag="ACIE68_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFX5909664.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            13571..14233
                     /gene="can"
                     /locus_tag="ACIE68_003401"
     CDS             13571..14233
                     /gene="can"
                     /locus_tag="ACIE68_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFX5909665.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(14274..14810)
                     /gene="hpt"
                     /locus_tag="ACIE68_003402"
     CDS             complement(14274..14810)
                     /gene="hpt"
                     /locus_tag="ACIE68_003402"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFX5909666.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
BASE COUNT         4124 a         3569 c         3417 g         3821 t
ORIGIN      
        1 gttttttaac accaaacata caacagcttt atgcttcgta acctgtatgg cttttagttc
       61 atcgtctatt gcggacattg tcatttcggg tactcgcgta atatataaaa gcgatcaaaa
      121 aagtgtcaac gtacgtctgg aaaataaagg gaataacccg ttgcttgtcc agagttggtt
      181 agatactggc gatgacaacg ctgagcctgg cagtattaca gtccctttta ctgccacgcc
      241 gccagtatcg cgtattgatg ccaaacgtgg gcaaacaatc aaattaatgt acacagccag
      301 cacctcactg cctaaagaca gagagagcgt gttctggttt aacgtactgg aagttccacc
      361 aaaaccagat gcagaaaagg tcgcgaatca aagtctgctg caactggcat ttcgcacacg
      421 tataaaactt ttctatcgcc cggatggatt gaagggaaat ccctctgaag ccccgttagc
      481 cctgaagtgg ttctggtcag gttcagaagg taaggcgtca ttgcgagtga ccaatccaac
      541 cccttactac gtctctttta gcagtggtga tttagaagct agcggtaaac gctatccgat
      601 tgatgtgaaa atgattgcac catttagtga tgaggtcatg aaagtcactg gccttaatgg
      661 caaagcaagt tctgcaaaag tacattttta cgccattaat gattttggtg gcgccattga
      721 aggtaatgcc agtctgtaat caggcaagga tataattccg caggaagcat aacgtgacta
      781 taaaaccaac taaaaattat cataatcatc tgacccgtat cgctacgttt tgcgcgctgc
      841 tgtattgcaa tagtgctttc tgtgctgaac ttgttgaata tgaccatacc ttcctgatgg
      901 ggcagaatgc ctcaaatatt gatctcagcc ggtatagtga aggtaaccct gctataccgg
      961 gtatgtacga cgtcagtgtt tatgtcaacg atcaacctat tattaaccaa agcattacat
     1021 ttattgaaat tgaaggaaaa aaaaacgcac aagcctgtat cacacttaag aatttattgc
     1081 agtttcatat aaattctccc gatataaata acgaaaaagc cgttctgctt gccagggatg
     1141 aaacactcgg caattgcctt aatttgacgg aaattatccc tcaggcttct gttcgttatg
     1201 acgttaacga gcaacgtctg gatatagacg ttcctcaggc atgggtaatg aaaaattacc
     1261 aaaactatgt tgatccatcg ttatgggaaa atggcattaa tgcggccatg ttgtcataca
     1321 acctcaacgg atatcatagt gaaactcctg gtcgaagaaa tgacagcatt tatgctgctt
     1381 ttaacggtgg gatgaattta ggtgcatggc gactgcgtgc ctcaggcaac tacaactgga
     1441 tgaccgattc tggcagtaat tatgatttta agaatcggta tatacagcgt gatatcgcct
     1501 cgctgcgttc tcaactcatt cttggtgagt cttatacaac gggcgaaacc tttgattctg
     1561 tcagtatccg cggcattcgt ttatacagcg acagccgcat gttgcctccg acattagcga
     1621 gttttgcgcc tatcattcat ggcgttgcta ataccaacgc caaagtcact attacgcaag
     1681 gtggctataa gatttatgaa acgactgtgc cgccaggcgc tttcgtcatt gatgatctga
     1741 gtccgtcagg ttacggcagc gatcttattg ttactgtcga agaatccgat ggctcaaagc
     1801 ggacattctc gcaacctttt tcatcggtcg ttcaaatgtt acgccctggc gttggacgtt
     1861 gggatattag cggcggccag gtcttaaaag atgatattca ggatgagcct aatttatttc
     1921 aggccagcta ctactacggt ctgaataact atctaacggg ttatactggt attcagatta
     1981 ccgataataa ctataccgct ggtttgttag gtcttggtct gaatacttca gtcggtgcat
     2041 tttctttcga tgtgactcac tccaatgttc gtataccgga tgataaaaca tatcagggac
     2101 aaagttatcg tgtttcctgg aacaagttat tcgaagaaac aagtacttca ctgaatatcg
     2161 cggcctatcg ctattcgaca cagaattacc ttggtcttaa tgatgcacta actctaattg
     2221 atgaagtgaa acatcccgaa caagatcttg aaccgaaatc catgcgtaat tactcgcgca
     2281 tgaaaaatca ggttacggtc agtattaacc aaccgctaaa atttgagaaa aaagattacg
     2341 gttcatttta tctttccgga agttggtccg attactgggc ttccggacaa aatcgtagca
     2401 attactctat tggctacagt aacagtgcat cctggggcag ctacagtgtc agtgcccagc
     2461 gttcatggaa tgaagacggc gacactgacg atagcgttta tctcagtttc accattccta
     2521 ttgaaaaatt acttggcact gaacaacgga cttcaggttt ccagagtatt gatactcaaa
     2581 tgagcagtga ctttaagggt aataaccaac tcaacgtcag tagcagtggc tatagtgata
     2641 acgctcgcct cagttatagc gtaaatactg gctatacgat gaataaagcc agcaaagatt
     2701 tgagttatgt tgggggttat gccagctatg aatcaccgtg gggaaccctg gcaggttcag
     2761 tttctgcaaa tagcgataac agtcgtcaag tgtctctcag caccgacggt ggttttgtat
     2821 tgcatagcgg cggcctgacc ttcagtaatg acagttttag cgactccgat acactggcgg
     2881 tagttcaggc tccaggtgct caaggagcgc gaataaatta tggcaacagt actatcgatc
     2941 gatggggtta tggtgtcacc agcgctcttt ctccttatca tgaaaaccgt atcgcgctgg
     3001 atatcaacga tcttgagaac gatgttgaat taaaaagtac cagtgcagta gctgtaccgc
     3061 gtcagggttc agtcgtcttt gctgattttg aaaccgtgca agggcaatca gccattatga
     3121 acatcacacg aagtgatggt aaaaatattc catttgctgc agatatttat gatgagcaag
     3181 gcaatgtcat tggtaatgtt ggacagggtg gacaagcatt tgttcgtggc attgagcagc
     3241 agggaaatat cagcattaaa tggctcgaag aaagtaaacc cgtaagttgt cttgcgcatt
     3301 atcaacaaag ttcagaagca gaaaaaatag cgcaatctat tattctgaat ggaattcggt
     3361 gtcagattga gtaactacaa gggacgtcaa atgataaaaa taacgccaca taaaatagcg
     3421 atactgatgg gaatattatt atcaccatca gtatttgcaa cggatattaa tgtagagttt
     3481 acagccactg tcaaagcgac aacctgtaac atcacactta ctggtaataa cgtcacgaat
     3541 gatggcaata ataactacac attgagaatc cctaagatgg gtctggataa gatcgcgaat
     3601 aaaacgacag aatctcaggc tgattttaaa ctggttgcta gtgggtgcag cagtggcatc
     3661 agttggattg ataccactct gaccggaaat gcatcatcaa gctcacctaa gcttattata
     3721 ccgcagtctg gtgattcatc ttcgacgaca agtaatatcg gtatgggttt caaaaaacgg
     3781 actactgatg atgccacttt ccttaaacct aacagtgcgg aaaagatacg ctggagcaca
     3841 gacgagatgc agcccgataa gggtcttgaa atgaccgttg cgctacgtga aacagatgca
     3901 gggcaaggcg taccggggaa ttttcgtgca ctggcaacgt ttaatttcat ctatcaataa
     3961 gaaggataat taagatgaca tttaaaaatt tacgttatgg attatccagc agtgtcgttt
     4021 tggctgcctc attgttcagc gtaccctctt atgcggcaac agacagcatt gggctgaccg
     4081 tgattactac cgtagaaata ggtacttgta ccgctacatt agtaaatgac tctgatcagg
     4141 acatttctgt tgttgacttt ggcgatgtat atatttctga aatcaatgcc aagaccaaag
     4201 ttaaaacatt caaactcaaa ttcaaagact gtgcgggtat ccccaataaa aaagcacaaa
     4261 taaaattaac caagcgagcc acatgcgaag gtactgctaa tgacggtgcg gggttcgcaa
     4321 atggatccac agccgcagat aaagcaagtg ccgtcgccgt tgaagtctgg agcaccgaaa
     4381 ctccggcaac aggtagcgca acacaattta gttgtgtgac accagcatct caagaggtca
     4441 ctatctccaa tgcagccaac gcggttgttt attatccgat gagtgctcga ctggtcgtcg
     4501 aaaaaaataa aaccgtaagc aatgtcactg cgggtaaatt ttctgcacct gccacattta
     4561 cagtaaccta taactaacag actgacagat ccaggaacta tatatgcatc ccactcaacg
     4621 taagctgatg aagagaataa ttctgtttct gtcactacag tttcccatta cctatccagc
     4681 catcgctggg caggatattg accttgttgc caatgtaaaa aacagcactt gcaaaagcgg
     4741 aatcagtaac cagggtaata ttgatcttgg cgtcgttggg gtgggatatt tttcagataa
     4801 tgttactcct gaaagctatc aaccaggtgg aaaagagttc actgtcacag tatcagactg
     4861 tgcattacag ggaactggcg atgtgcttaa tcagttacat attgatttta gagcccttag
     4921 cggtgtcatg gctgcaggtt caaggcaaat atttgccaat gaagtcgcaa ccggggctaa
     4981 aaatgtcggt gtcgttctct tttccattca ggacccgacc aatatattca atgttattag
     5041 ttcagcagga aattctcgtt ccgtgtatcc agtaatgact tctgccctgc ataattcttc
     5101 atggaaattt tatgccagaa tgcaaaaaat cgatcccgcg ctggatgtca tatctggcca
     5161 ggtaatgagt catatattag ttgatgtgta ctacgaataa tagatcaaaa caaataactg
     5221 cgactgttac agcagtagat ggtggagtga taatgaagaa attcttcaga cattttcttt
     5281 ttttaattct gtgcttgagt tgttataccg cgagtgctgg cactgatgat aacgtaggat
     5341 atattgttgg gaatagttac ggcgttggtc cgtcagatca aaaatggaga gaaacggggc
     5401 cgaatggcga tgctactgtc atattcagat atgcaacaag tacaaataat ttagttttct
     5461 acaaaccgac gcagcttgga cctacaggtg taaaattaca gtggagtcag ttagataccg
     5521 cttctggtgg tggttttctt tattgcaaca gaagtgatag cacaagtggt agcgcaatgc
     5581 gtattgaaaa tgcaatggtt gactcaggta aaatgtatgg ctcccataaa ttatttaata
     5641 catcagttcc tggtttgtat tacacattat taatttcaaa catgtggtca gcttacggta
     5701 ccgtaactaa cgttagttca cctgggatat acattggtga ctctgcagaa caatattttt
     5761 cgtggtataa tccaagcgaa gacgtgttat attggagttg caataatgcg aatagcaccc
     5821 gtaaatactg ggctgtaggt ggtatttatc agacccttac aattgaattc tatacagata
     5881 caaactttga tccaacagta actcaacaga tcaaattatc cagctcatca aattatctgt
     5941 attcatttaa agcctatggc gcaggccaag gtataaatga gcatagttat tttatcaaaa
     6001 tcgatttcga tctgctcaat gtcaagttga ctaatcccac ttgttttacc gctatgctta
     6061 gtggaacttc tgtgactggt tctacggtaa aaatgggtga atatagtgca gaacaaatca
     6121 gaaacggtgc cacaccggtt ccttttgata tttcacttca aaactgcgtt cgtgtgacta
     6181 atattgagac aaaattagtt tcaacaaagg ttggtactga aaacgggcaa ctccttggta
     6241 atactctcac aggtaatgac gcagcaaaag gagtcggtgt actcatagaa ggtttagcaa
     6301 ctagtaaaaa tcctctaatg acattgaaac ctaatgattc aaattctgtt tataaagatt
     6361 acgacccgag aggcaaagac gatacaacag gaggagttta cccggatcaa gatacaggta
     6421 taacataccc tctccatttc caggccacgc tacaacagga tggaactata ccaatagaag
     6481 ctggtgagtt taaagccacc agtactttcc aggtaaccta cccttaataa gtccaccgca
     6541 ccgccatcat ctggcggtgc agtaattgat aaatcattgc ccccaccaat gtgactgata
     6601 aaatgccagg caacacgaca tcatttatca ggatacgtta tgaaaccaac caccattgct
     6661 tcacttcaga aatgtaagca ggataaaaag cgtttcgcga ccatcaccgc ttacgactac
     6721 agcttcgcga aactatttgc tgatgaagga cttaacgtca tgctggtagg cgactcgctg
     6781 ggcatgacgg ttcagggtca cgactccacc ctgccggtga ccgttgctga tatcgcctac
     6841 cacactgccg ccgtacgtcg cggcgcgcca aactgcctgc tgctggctga tctgccgttt
     6901 atggcgtatg ccacgccgga acaagccttt gaaaacgccg caacggttat gcgtgccggt
     6961 gccaatatgg tcaaaattga gggcggtgag tggctggtcg aaaccgtaaa aatgctgacc
     7021 gaacgtgccg ttcctgtatg tggtcactta ggtttaaccc cacagtcagt gaatattttc
     7081 ggtggctaca aagttcaggg gcgcggcgat gaagcgggcg atcaactgct cagcgatgca
     7141 ttagccttag aagccgctgg ggcacagctg ctggtgctgg aatgcgtgcc ggttgaactg
     7201 gcaaaacgta ttaccgatgc actggcgatc ccggttattg gcattggcgc aggcaacgtc
     7261 actgacgggc agatcctcgt gatgcacgac gccttcggca ttaccggcgg tcacattcct
     7321 aaattcgcta aaaatttcct cgccgaaacg ggcgacatcc gcgcggctgt gcggcagtat
     7381 atggctgaag tggagtccgg cgtttatccg ggcgaagaac acagtttcca ttaaggagtc
     7441 acgttgtgtt aattatcgaa accctgccgc tgctgcgtca gcaaattcgc cgcctgcgta
     7501 tggaaggcaa gcgcgtggcg ctggtgccta ctatgggtaa tctgcacgat ggccatatga
     7561 agctggtcga cgaagccaaa gcccgcgccg atgtggtcgt cgtcagtatt ttcgttaacc
     7621 caatgcagtt tgaccgcccg gaagatctgg ctcgttatcc acggaccttg caagaggact
     7681 gcgaaaagct aaacaaacgt aaagtggatt tagttttcgc cccttcggta aaagagatct
     7741 acccgaacgg tactgaaacc cacacttacg ttgacgttcc tggcctttcg accatgctgg
     7801 aaggtgccag ccgtccggga cattttcgcg gcgtttcgac tattgtcagc aagctgttca
     7861 acctggtcca gccggacatc gcctgcttcg gtgaaaagga ctttcagcaa ctggcgctaa
     7921 tccgcaaaat ggttgccgat atgggcttcg atattgaaat tgtcggcgtc ccgattatgc
     7981 gcgccaaaga tggccttgcg ctcagttccc gtaatggtta tctgacggcg gaacaacgca
     8041 aaattgcgcc cggtctgtac aaagttttaa gttcaattgc cgacaaattg caggctgggg
     8101 aacgggatct cgatgaaatt attaccattg cggggcaaga actgaatgaa aaaggcttcc
     8161 gcgccgatga tattcagatt cgcgatgccg acacattgct ggaagtttct gaaaccagca
     8221 aacgggcagt aattctggta gccgcctggc ttggcgatgc tcgcctgatc gacaacaaaa
     8281 tggtcgagct ggcgtaatac ttaactggcg ctacggctga tggcgccagt tattaattta
     8341 ccccacgtca tctgtcataa cacaggtaac ttaaataagg ttaattacct tatcaatctc
     8401 agcaagagat aaatttgtca tctctgcaac gtcttcccga gacattcctt tacttagaag
     8461 acgcagggcg aattcttgcc tttcctcctg tcttccctgc tgaatcccct tctcaatccc
     8521 tttctcttca aaccactgcg ccagcgtcat catagactcc cctcccgttt ccctgtctct
     8581 cagcacacca taaaacaaat ccgcttgttc agtatgaccg cgttgcagca tatagttttg
     8641 catggcaact aactgacttc cgctagtgta cccttcgtca atcagcgtga ccagttgctc
     8701 cagcaataac attaagtcgc gctggcgaat atgtttttgc agtagttcga gaatcgcaat
     8761 ccgccgatgt tgcatgattt cgtcatccgg tgtgatggtg atatccacca gcgggaaagg
     8821 actgttatag acgcgtcgtg ccagctccgg cgagtaaaat atatcaaacc agcacattga
     8881 gagcggataa ggtgtggcct cgccctgata aaacagtatc ggcaccacca gcggcagctt
     8941 atcgtgatcc gcctccagat gccggtgcat ggcagcaata gaataacgca tcatgcgaaa
     9001 ggccattttc ttatccggct tgctttggtg ttcaatcaca acatgcagat aaccgggatt
     9061 gccctgcatt tgcacggaat agagcacgtc cgtgctgtgt cctttcaggc tctcttcaat
     9121 gaaactcccc gactctaaat gaagcgtgtt gaggtcacaa agttcgcgta attccactgg
     9181 caaatgtatc tccagaaagt cgcgagccgt ctccgcatgc attaaaaatt gtttaaatac
     9241 cgcgtcatgc ggtgtggtac tcggtgcatc catcttccct gtcttccgtg ttgtcttgcg
     9301 tgaaggcagt atccagtcaa ggcgcgccgt cgtcagccgc ctctttagtg tttaccgagc
     9361 agcgttcaga gaaatttcaa ttcgttacag gcgatacatg gcacgcttcg gcgcggtacg
     9421 caaactgctg acgcaaatgc catcagatga gtagacactc atcaaaaatc gggcaatact
     9481 gcgtgagaat ttcctcaaag caggcaccgc ctgcttcgtt aacgacaggg tagaaaggta
     9541 gaagttatga ttcgcacgat gctgcagggc aaactccacc gcgtgaaagt gactcatgcg
     9601 gacctgcact atgaaggttc ttgcgccatt gaccaggatt ttcttgacgc agccggtatt
     9661 ctcgaaaacg aagccatcga tatctggaat gtcaccaacg gcaagcgttt ctccacttat
     9721 gccatcgcgg cagaacgcgg ttcgagaatt atttctgtta acggtgcggc ggcccactgc
     9781 gccagcgtcg gcgatattgt catcatcgcc agcttcgtta ccatgccaga tgaagaagct
     9841 cgcacctggc gacccaatgt cgcctatttt gaaggcgaca atgaaatgaa acgtaccgcg
     9901 aaagcgattc cggtacaggt tgcttgattg ttatccctgc ggctggttac tcatcagccg
     9961 cgacatcgtc tccagcgaat ccgttcttaa gatataaagt cgttttagta acaacggatt
    10021 atcccccggt tttactttgc ctttcatggt tgtcaccgcc aggtgaaatc cggcatcgtt
    10081 tgctgccttc acggctttgt cattaaatcc gccaaacgga tacgaaagat accagacatg
    10141 cggattaaat tgcgccagag cgcggcgtga acgtgcaaaa tcaaacagaa tattgtgctc
    10201 actacggctc agtaatatgg gtcggcgata gccatccacc cgatgcaaaa aatgggtatg
    10261 tgactgaaaa tcaaaaacat cgcgaatttc gttaagctca gaaacgctca taaattgcag
    10321 cgattttggg ttccacttct gcgggtgacg tttgatgcgc gaggtaacaa caaacgccgt
    10381 cgccttcatg ccatattgtt tcaatacagg atacgcatag cggctcaccg acttgaggcc
    10441 atcatcaaag gtaatcacca ccgctcgcgc agggagattg atcttattct tcacgtaacc
    10501 ttccagctgc gccatgctca gtgtcgcgta tcccctgtcg cgcagccagg ccatctggtt
    10561 attgaaagcg cgtactgagg tggtcgtcga agtatggcga aaacgggtgt tttcttcatc
    10621 gcgcagaata tggtgatagg ttagcaccgg caggccatta tcgggttggg catccagcgc
    10681 gctgatatag gcgagtcgat cgccaatacg gatctgatac caggtttgat ttaacctgtc
    10741 tttcagttta tgcaaaatcg ggtagcgcaa attgtccgcc agtaccccaa atggcgcact
    10801 gcccgcactc ggcgcgttat agaccggcgt atctttccag gtaattaagt tctgattact
    10861 cagcggcttg ttgaggtcac ccaaaccgtc ttcaactttt tgtcgcccct gaactggctc
    10921 gagatgccct ttatcgataa accctttacc aaagccaaaa ttaaatgcgt aataacttgc
    10981 ggcagtgggc tccacggcaa tgatttgccc ggcccgaata ttccccacgg tcaccatctt
    11041 gtcaccaatt tgcgcccaga ccgcagcatt ttcgatggtt tgcatataac gggcaggtaa
    11101 cgcggcacta acgcttgcag taaacagcat cagcaggaga ataacagctt gtttgtacat
    11161 gaaaaaataa ccgagggtga aacaacgctt ttattgtagc aaaagcgcca tcagaatcgc
    11221 gggttaatac tcatacaaat tatgtttgag gacgaaaggc ggattttttt gttgttggtg
    11281 ccaaagacta ctcacctccg gcgcacccag ttcgacaata cgctcgcgaa actctgaact
    11341 ggtcatggtt tcacggcaag ccatcatctg ttcaattaac ggtaacgtga caccagaaat
    11401 cacttcgcaa cgggagtgtt tgtggcttaa taatgaagcc acgcggtacg gtggcgctcc
    11461 ggctatatcg gttaagaaga tgacaccctc accagagtcc gcttcatgca gagcatcgca
    11521 catcatgcgg ctgagcatat tagagcttaa cccacgccag aaattcacgg cccggcactg
    11581 aagaagcgcc ccatgttttt tctccagcgc atccagtatc tcttgcgccc ggtcatcgtg
    11641 acaggtaatt acccaaccta acatcgcctt cctccttagc cgaaaatcag tctaccctga
    11701 ccattgttga taaagggtga tagccgtcaa aactcctctc ccccaaatcc agaggagagg
    11761 aaatagcctt agctacgcaa accacgtcca cgttggatca gcgaccaaca gatcaaataa
    11821 aacgccacaa taaagaccac cagtacgcca aaggtagtga ccagcggaac atcattgata
    11881 ccgaggaagc cgtagcggaa accactgatc atataaacga ttgggttcag gtgcgacagc
    11941 ccttgccaga acggcggcaa caaagtcagt gagtaaaaga ccccacccaa atacgtgagt
    12001 ggcgttaaca caaaggttgg caccaggctg atgtcatcga acgttttggc aaacacaccg
    12061 ttcagcaaac ccgcaaggga gaacaacacc gccgtgagca ccagggttaa ggcaacgaat
    12121 acccacgaat gcacctgaaa cggcacaaaa aacagtgaaa ttgccgtcac cagaatgcca
    12181 acaaacagac cacgcgccac gccaccgccg acatatccgg caataatgac gtgagtcgga
    12241 accggcgcta ccagcagctc ttcaatatta cgctggaact tggcaccaaa aaatgatgac
    12301 gcaacgttgg cgtaggcatt ggtgatcacc gacatcatga tcagccccgg tacgatgaac
    12361 tgcatatagc tgaagccatg catatcgcca atacgcgaac caatcaggtt accgaagata
    12421 ataaagtaaa gggtcatggt gatgactggc ggcaccagcg tctgcaccca gatacgcata
    12481 aagcgatgga tctctttcgc ccagatgctt tttagcgcca cccagtaaag atgcatcatg
    12541 cgcgatctcc ttgtttttca ttaaccagtg aaacaaacag ctcttccaga cggttagctt
    12601 tgttacgcat acttaatacc tgaatgccct gctcacttaa ctgcgtaaat acgctgttga
    12661 tcccctgctc acgcagcact tcaacttcca gcgtcgcggt atcgaccagt cgatactgat
    12721 agccatcgag cttcggtaac ggacttttcg gtgcgagatc gagaataaag gtttccgatt
    12781 tcagcttcgc cagcagcgcc ttcatcgagg tattttccac cagctcaccg tgttgaataa
    12841 tgccgatatt gcggcacagc atttctgctt cttccaggta gtgtgtggtg agaatgatgg
    12901 tggtgccttt gtcgtttaaa tccttcaaaa agccccacat tgagcggcga agttcaatat
    12961 ccacgcctgc ggtcggttcg tcgagaatca gtagtttagg ttcatgcatt aacgcacggg
    13021 caatcattaa acggcgcttc atcccgccag ataacatacg cgcacgttcg ttgcgttttc
    13081 cccatagatc gagttgttta agatattttt cgctgcggat gtacgcctct ttgcgctcca
    13141 caccgtagta ccctgcctga ttcaccacaa tttgctgcac ggtttcaaac gggttgaagt
    13201 taaattcctg cggcaccagt cccaactgac gtttagcgtt cacgacatct ttctcgagat
    13261 cgtaaccaaa tacgctgacc cgcccggagg ttttatttac cagagagctg ataataccga
    13321 tagtggtcga tttcccggcc ccgttcggcc cgagaagcgc ataaaaatca cccgcttcga
    13381 cctgcaaatc tattccacga agcgcctgaa cgccgcctgg ataggttttt ttaagctgtt
    13441 gaagttccag tgcaatggtc ataaattttt acttacctta cgttcttaca ctttatctgt
    13501 ggtttaaatc gtcccggagt tgccctatat tagccaaacg taattatttg gttacaggtc
    13561 gttaacctcc atgaaagaca tagatacact catcagcaac aatgcactat ggtcaaaaat
    13621 gctggtggaa gaggatcccg ggttttttga gaaactggca caagcgcaaa aaccgcgctt
    13681 tctatggatt ggatgttccg acagtcgcgt tcctgcagaa cgtttaaccg gtcttgagcc
    13741 gggcgaactc tttgttcacc gtaatgttgc taacctggtc attcacaccg acctgaactg
    13801 cctttccgtg gttcagtatg cagtggatgt actagaagtt gaacacatta ttatctgtgg
    13861 ccactacggt tgcggcggcg tacaagccgc agttgaaaac ccggaactgg ggcttatcaa
    13921 caactggctg ctgcatatcc gcgatatctg gttcaaacat agctcattgc tcggcgaaat
    13981 gccgcaagag cgccgtctgg ataccttgtg tgaactgaac gtcatggaac aggtgtataa
    14041 cctgggccac tccaccatta tgcaatcagc gtggaaacgc gggcagaaag ttaccattca
    14101 cggctgggcc tatggcattc acgacggctt gctgcgtgat ctggatgtca cagcgactaa
    14161 ccgcgaaacc cttgagcaac gttaccgtca cgggatttcc aacctcaagc tgaaacacgc
    14221 caaccacaaa taaaaatgcc atgccggatg caacacatcc ggcaacttca cacttactcg
    14281 tccagcagaa tcactttgcc gatatacggc agatgacggt aacgctgtgc gtaatcaatg
    14341 ccgtaaccca ccacaaactc atccgggatc gagaaaccga taaattctac cgggacgttc
    14401 acttcacgac gggacggttt atccagcagc gtacaaatcg ccagcgactt cggttcgcgc
    14461 aggcttaaga tctcacgcac tttcgacagt gtattccccg agtcgatgat atcttcaaca
    14521 atcagcacgt ccttgccacg gatatcttca tccagatctt tgaggatttt cacatcacgg
    14581 gtggtggaca tgccgctacc gtagctggag gcggtcataa agtcgacttc atgagatacc
    14641 tgaacttcac ggcacaggtc cgccataaac ataaatgagc cacgcagcag accaaccagc
    14701 accatatcgc tgccgctgtc tttgtaacgc tcagtaatct gacgacccag ttcggcgata
    14761 cgcgctttaa tctccgcttc ggggatcatt acttctacag tatgtttcat atctctaacc
    14821 atatgattta aaaacaaatc acttcgcacg ggcaggtttt ggcaccgcaa caaagcgcaa
    14881 gacacgcagt ataccagtaa aactattcat tcgcggaatc tgttaataaa g
//
LOCUS       DBANUU010000107        14738 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000107 DBANUU010000000
VERSION     DBANUU010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.107"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            157..5577
                     /locus_tag="ACIE68_003403"
     CDS             157..5577
                     /locus_tag="ACIE68_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
                     /protein_id="HFX5909667.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGMGEATIREGGLVTAENTIIGGNATGV
                     GTLNVQDQDSVITVRRLYNGYFGNGAVNISNNGLINNKEYSLVGVEDGSHGVVNVTDK
                     GHWSFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGENGVGNVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDGTSSFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGTGEL
                     NITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLN
                     VEGGEVYLGVFEPAVGTLNIGAAHGEAAADAGYITNATKVEFGSGEGVFVFNHTNNSD
                     AGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMG
                     SSEVTIASPGTLDILASTNSAGDYTLTNALKGDGLMRVQLSSSDKMFGFTHATGTEFA
                     GVAQLKDSTFTLERDNTAALTHAMLQSDSENTTSVKVGEQSIGGLAMNGGTLIFDTDI
                     PAATLAEGYISVDTLVVGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLN
                     LLEHDDSHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGF
                     RLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVR
                     QVSLSNGQNDYQGATYVQMGTLRTDADGALGNTRELNISNAAIVDLNGSTQTVETFTG
                     QMGSTVLFKEGALTVNKGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSISPNAE
                     VSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVG
                     QFNIDTGSALSVNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDVTKLGTGILT
                     LNNDSAAYQGTTDIVGGEIAFGSDSAINMASQHINIHNSGVMSGNVTTAGDVNVMPGG
                     TLRVAKTTIGGNLENGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPT
                     DKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVY
                     TLAKGKGNDEKNWYLTSKWDGVTPPDTPDPINNPPVVDPEGPSVYRPEAGSYISNIAA
                     ANSLFSHRLHDRLGEPQYTDSLHSQGSASSMWMRHVGGHERSRAGDGQLNTQANRYVL
                     QLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGIYATWYQ
                     NDANKTGAYVDSWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEG
                     TLNTWYVQPQVQITWMGVKDSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDG
                     KQREFQPYIEANWINNSKVYAVKMNGQTVSRDGARNLGEVRTGVEAKVNNNLSLWGNV
                     GVQLGDKGYSDTQGMLGVKYSW"
     gene            5786..6052
                     /gene="hipB"
                     /locus_tag="ACIE68_003404"
     CDS             5786..6052
                     /gene="hipB"
                     /locus_tag="ACIE68_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFX5909668.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            6052..7374
                     /gene="hipA"
                     /locus_tag="ACIE68_003405"
     CDS             6052..7374
                     /gene="hipA"
                     /locus_tag="ACIE68_003405"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFX5909669.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            7686..7894
                     /locus_tag="ACIE68_003406"
                     /pseudo
     CDS             7686..7894
                     /locus_tag="ACIE68_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            8326..8889
                     /gene="fimA"
                     /locus_tag="ACIE68_003407"
     CDS             8326..8889
                     /gene="fimA"
                     /locus_tag="ACIE68_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFX5909670.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            9250..9960
                     /locus_tag="ACIE68_003408"
     CDS             9250..9960
                     /locus_tag="ACIE68_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFX5909671.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            10002..12653
                     /locus_tag="ACIE68_003409"
     CDS             10002..12653
                     /locus_tag="ACIE68_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFX5909672.1"
                     /translation="MTAIRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSATNGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRNGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            12667..13197
                     /gene="ydeS"
                     /locus_tag="ACIE68_003410"
     CDS             12667..13197
                     /gene="ydeS"
                     /locus_tag="ACIE68_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5909673.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            13210..13713
                     /gene="ydeR"
                     /locus_tag="ACIE68_003411"
     CDS             13210..13713
                     /gene="ydeR"
                     /locus_tag="ACIE68_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5909674.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            13773..14687
                     /gene="ydeQ"
                     /locus_tag="ACIE68_003412"
     CDS             13773..14687
                     /gene="ydeQ"
                     /locus_tag="ACIE68_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5909675.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         4116 a         3139 c         3687 g         3796 t
ORIGIN      
        1 tatcaattcg aaagcattag caaattagcc aaattgacac cttcccacct ccaactaaca
       61 ctcaatacag acctctgaca atttaataag tttcattttt aatgtctgac gactcatctc
      121 tttcgtggct gtattgtttg tatatggagc gtttatatga atagaatcta tcgcgtgata
      181 tggaattgca ctctacaggt atttcaggcc tgctcggaat taactcgcag ggcaggtaaa
      241 acatcgacgg ttaatttgcg taaatcctct ggactgacaa cgaaattcag tagattgacg
      301 ctgggtgttt tgctggcact aagcggttca gcgtctggtg caagtctgga agttgataat
      361 gatcagatta ccaatattga tactgatgtt gcttatgatg cctacctggt tggctggtat
      421 ggcactggag tgcttaatat tttggctggc ggtaatgcct ccttaaccac tattactacc
      481 agcgtcattg gcgctaatga ggactcagag ggcaccgtta atgttttggg tggcacctgg
      541 cgattgtatg atagcggaaa taatgcaagg cctttaaatg tgggtcaatc cggaacgggg
      601 acgctgaata ttaaacagaa gggtcacgtc gatggaggct atttaagatt aggttcttcg
      661 acaggaggcg tcgggacggt caatgttgag ggagaggact ctgttctgac gaccgaatta
      721 ttcgaaatag ggagttacgg tacgggctca ttaaatatta cggataaggg ttacgtcacg
      781 agttcaatcg tcgccatttt aggctatcag gcgggcagta atggccaggt tgtcgttgaa
      841 aagggtggcg agtggctaat aaaaaataac gattcctcaa ttgaatttca aattggtaat
      901 caagggatgg gggaagcgac tattcgcgag ggggggctgg ttacggctga aaatacgatt
      961 atcggtggca atgccaccgg tgtcggaacc ctgaatgtgc aggatcaaga ctctgtcatc
     1021 acggtacgca gactctataa tggatatttc ggtaatggcg cagtcaatat ttccaataat
     1081 ggactaatta ataacaaaga atattcattg gtgggcgttg aagacggttc ccacggtgtc
     1141 gtcaacgtga ccgataaagg gcattggagt ttcctcggaa caggcgaagc tttccgctat
     1201 atctatatcg gtgatgctgg tgacggtgaa cttaatgtct cgcgtgaagg taaagtagat
     1261 tcgggaatta tcactgcggg gatgaaagaa acaggcacag gcaacattac tgttaaggat
     1321 aagaactccg ttatcactaa tctcggaact aatcttggtt atgacggcca cggcgaaatg
     1381 aatatcagta atgaggggct tgttgtcagc aacggaggaa gctcactcgg ttatggagaa
     1441 aacggcgtcg ggaatgtcag catcaccacg ggggggatgt gggaggtcaa taagaatgtc
     1501 tataccacca ttggtgttgc gggcgtcgga aacctcaata ttagcgatgg cggtaagttt
     1561 gtatcgcaaa atattacttt tttgggcgat aaagcaagcg gtatcggcac actgaacctg
     1621 atggatggga catcatcgtt cgatactgtg ggtatcaatg tcggtaattt tggtagcggt
     1681 atcgtaaatg tcagtaatgg tgccaccctt aattcaacgg gctatggatt tatcggagga
     1741 aatgcctccg gtaaagggat agttaatatt tcaacggata gtctctggaa tttaaagaca
     1801 tcatctacta acgcccaatt gctacaggtc ggtgtattag gcacgggtga actgaatatt
     1861 accaccggag gtatagttaa agcgcgtgat acacagatag ctctcaatga caaaagtaag
     1921 ggcgacgtga gggtggatgg gcagaactct cttcttgaaa cattcaatat gtacgtaggg
     1981 acatctggta cgggtacgtt aaccctgacg aatagcggca cgctgaatgt cgaaggtgga
     2041 gaagtttact taggtgtttt tgaacctgct gtaggaacgc taaacattgg tgcagctcac
     2101 ggtgaggcgg cggcagatgc cgggtatata accaacgcga cgaaagtaga gtttggttct
     2161 ggcgaaggtg tttttgtctt taatcatact aataacagtg atgccggcta ccaggtcgat
     2221 atgctgatta cgggtgacga taaagacgga aaagtgatcc atgatgcagg ccatacggtg
     2281 ttcaatgcag ggaatactta tagcggtaaa acgctggtca atgacggcct cctgaccatt
     2341 gcgtctcata cggcagatgg ggtaacgggc atgggttcga gtgaagtcac cattgcaagc
     2401 cccggtacgc tcgacattct cgcatcaacg aacagtgcag gagattacac gctgaccaat
     2461 gcgctcaaag gcgatggttt gatgcgagtg cagctgtcat cctccgacaa gatgtttggc
     2521 tttacccatg caacagggac tgaattcgcc ggtgttgccc aactgaaaga cagtaccttc
     2581 actctggaac gcgacaacac cgctgcgctt actcacgcga tgttgcagtc tgacagtgaa
     2641 aataccacat cggtaaaagt gggagagcaa tccattggtg gactggccat gaatggcggt
     2701 acgctcattt tcgatacgga tattcctgct gcgacgcttg cagagggata tatcagcgtc
     2761 gatacgctgg ttgtcggcgc gggtgactac acctggaaag gccgtaacta tcaggtaaac
     2821 gggacgggcg acgtgcttat cgacgtgcct aaaccgtgga atgatcccat ggcgaataac
     2881 cctctgacga cgctcaattt gctggaacac gacgatagcc atgtcggcgt tcaactggtg
     2941 aaggcgcaaa cggttattgg gtcgggtggc tcattaacgt tacgtgattt acagggcgac
     3001 gaggtggaag cggacaaaac gttacacatt gcgcaaaacg gaacggtggt cgccgagggt
     3061 gattatggat tccgcctcac gaccgcacca ggtgatggtt tgtacgttaa ctatgggctg
     3121 aaagcgctga acatccatgg tgggcaaaag ctgacgttag ccgaacatgg cggagcctat
     3181 ggcgcaacgg ccgatatgtc ggcaaaaatc ggtggtgaag gggatctggc aatcaatacg
     3241 gtgcgacagg tttcgctttc caacggtcag aacgactatc agggggcaac ctacgttcag
     3301 atggggacat tacgtaccga tgcggatggt gcacttggca acacccggga actgaacatc
     3361 agcaacgcgg ccatcgtcga tcttaatgga tcgacgcaga cggtagagac attcaccggg
     3421 cagatgggtt cgactgtttt gttcaaagag ggggcgctga cggtaaataa aggtgggatc
     3481 agtcagggtg aactgacagg tggcggaaac ctgaatgtta cagggggaac gctggctatc
     3541 gaggggctta atgcacgcta caatgcgtta accagcatta gcccaaatgc ggaagtcagc
     3601 ctcgataata ctcaggggtt aggcagagga aatattgcca atgacggtct gttaacgcta
     3661 aaaaacgtga ctggcgaact gcgtaatagc ataagcggga agggtatcgt gagcgcaacc
     3721 gccaggacag atgtagagct ggatggcgat aatagccgct ttgtggggca attcaacatt
     3781 gatacaggca gcgcgctcag cgtcaacgag cagaaaaacc tgggtgatgc ttccgttatc
     3841 aataatggcc tgctcaccat ctccactgag cgtagctggg cgatgacgca cagtatcagc
     3901 ggtagcggtg atgtgacaaa actgggtacc gggatcctga ctcttaacaa cgattccgcg
     3961 gcgtatcagg gtacgacgga tatcgtgggg ggggaaattg ctttcggttc cgactctgcc
     4021 attaatatgg caagtcaaca cattaatatc cataacagcg gtgtgatgtc gggaaatgtc
     4081 accactgcag gtgatgtgaa cgttatgcct ggggggacac tgcgtgtcgc taaaaccaca
     4141 atcggcggca acctggagaa tggcggcacg gttcaaatga atagcgaagg ggggaaaccg
     4201 gggaatgtac tgaccgttaa cggcaactat accggaaaca atggcctgat gacgttcaac
     4261 gcgacgctgg gcggcgataa ttcgcccacc gataagatga acgtgaaagg cgatacccaa
     4321 gggaacactc gcgttcgggt tgataacatt ggcggcgtcg gtgcacaaac ggtcaacggt
     4381 attgaactca ttgaggttgg cggtaattct gcaggtaact tcgcgctgac aaccggaact
     4441 gtcgaagctg gggcttacgt ctacacgctg gctaaaggga aggggaatga cgagaaaaac
     4501 tggtatctga ccagtaaatg ggacggcgta acgccaccgg atacacccga ccccatcaat
     4561 aatccccctg ttgtggatcc ggaaggccca tcagtttatc gcccggaggc cggaagctat
     4621 atcagcaaca ttgccgcagc caactcgctg tttagccatc gtttacacga ccgtttgggt
     4681 gagccgcagt atacagattc actgcattct caggggtcgg caagcagtat gtggatgcgt
     4741 catgtcggag ggcacgaacg ttcaagggcc ggtgacggtc agctaaatac tcaggctaac
     4801 cgctatgtat tgcagctagg cggcgatttg gcgcagtgga gtagcaacgc gcaggatcgc
     4861 tggcatcttg gcgtgatggc aggctacgcc aatcagcaca gtaatactca gagtaatcgt
     4921 gtgggttata aatcggatgg gcgcatcagc gggtacagcg ctgggattta cgcgacctgg
     4981 tatcagaacg atgcgaataa gaccggcgct tatgttgaca gctgggcgct gtataactgg
     5041 tttgataaca gcgtcagttc cgataaccgt tctgctgacg actatgattc tcgcggtgtg
     5101 acggcctctg ttgagggtgg gtataccttt gaagcgggaa catttagcgg cagcgaaggg
     5161 acgctgaata cctggtacgt ccagccacag gtgcaaatca cctggatggg tgtgaaagat
     5221 tctgaccata cccggaaaga cggaacacgc attgaaacgg aaggcgacgg aaacgtgcaa
     5281 acgcgacttg gggtgaaaac ctacctgaac agccatcacc agcgtgacga tggtaaacag
     5341 cgtgagttcc agccttacat tgaagcgaac tggatcaaca atagcaaagt ctacgccgtg
     5401 aagatgaatg gtcaaaccgt aagccgtgat ggtgcgcgta atctcggtga agtacgtacc
     5461 ggggttgagg cgaaagtaaa taacaacctt agcctgtggg ggaatgtcgg tgtgcaacta
     5521 ggtgataaag gctatagcga tactcagggc atgctgggag tgaaatatag ctggtaaacc
     5581 gtataagccg catgtcgaga tggcatgcgg cttaatattg ccgacttcaa aggttttaaa
     5641 gcctcccttt tatccgtgat cgcggatatc gcagcgttta tcccgtacag cggataagat
     5701 gtgtttccag attgacttta tcctcactaa aggataaaac tgataatatc cccttaagcg
     5761 gataaatttg ctgtggacgt atgacatgat gagctttcag aagatctata gcccaacgca
     5821 attagcgaat gcaatgaaac tggttcgcca gcaaaatggc tggacgcaga gcgagctggc
     5881 gaaaaaaatt ggcattaagc aggcgacaat ttccaatttc gaaaacaacc ctgacaatac
     5941 cacgctcacg acatttttta agattttaca gtcgcttgaa ctctcaatga cactatgcga
     6001 cacgaaaaat gcctcgccag aatcaacaga acagcaagat ctggagtggt aatgcctaaa
     6061 cttgtcactt ggatgaacaa ccagcgggta ggcgagttaa cgaagttagc caacggcgcg
     6121 cacaccttta agtatgcacc ggagtggtta gccagccgtt atgccagacc gttgtcactt
     6181 tcgctgccat tgcagagggg gaatatcacc tcagatgccg tatttaactt cttcgataac
     6241 ctgttacccg atagcccgat tgtacgtgac cggatcgtta aacgttatca tgctaaatcc
     6301 agacaaccgt ttgatttatt gtcagaaata gggcgagaca gcgttggtgc cgtaacgtta
     6361 ttacccgaaa atgaaaccat aacgcgcccg ataatggcat gggaaaagct tactgaagcc
     6421 aggcttgaag aagtattaac ggcttataaa gcagatatcc ctctgggcat gattagagaa
     6481 gaaaatgact ttcgcatctc ggttgctggc gcgcaggaga agacagcgct gcttagaata
     6541 ggcaatgact ggtgcattcc gaaaggaata acgccgacga cgcacatcat taaattaccg
     6601 attggcgaaa tcaggcagcc caatgcgacg ctcgatctca gccaaagcgt tgataatgag
     6661 tattactgtc tgctgctggc gaaagaactt gggttgaatg ttccggacgc agaaatcatt
     6721 aaagcgggaa gggtgcgcgc gttagcggtc gaacgttttg acaggcgttg gaatactgag
     6781 cgaacggttt tacttcgctt gccacaggag gatatgtgtc agacattcgg tttaccttca
     6841 tctgtgaaat atgaatcaga tggaggccca ggcatcgcgc ggatcatggc gtttttgatg
     6901 gggtccagcg aggcgcttcg cgatcgttat gattttatga aattccaggt cttccagtgg
     6961 ttgattggcg caacggatgg ccatgcaaaa aacttctccg tatttattca ggctggcggc
     7021 agttatcgac tcacgccatt ttacgacatc atttcagcat ttccggtcct tggcggtacg
     7081 ggaatacaca tcagcgatct caaactggca atggggctta acgcatccaa aggcaaaaaa
     7141 acggcaatcg ataaaattta tccgcgacat tttttggcga cagcaaaggt gctgagattc
     7201 ccggaagtgc agatgcatga aatcctaagt gactttgcca gaatgattcc ggcagcactg
     7261 gataacgtga agacttcatt accgacagat tttccggaga acgtggtgac ggcagttgaa
     7321 accaatgtgt tgaggttgca tggacggtta agccgagaat acggtagtaa gtgagatatg
     7381 gggtttggtc gttgttaatg agcacgacaa tcatgaccgc caacgactgg attcttcctt
     7441 ggtttatact ggtgcgcata atgtatatta tgttaaatca tgtctgagtg atgcgaagtg
     7501 cttttctggt agtcgttatt cgttcagggt tcaagaactt aagcctctcc tctttcataa
     7561 ttggcctgtg catggagaaa tagcctggtt attccctgcc ccatgctaag agcgcagtgt
     7621 ctgtcgcata gacactgcat acgaaaacac cagagggtgc tgttacttat ttaaggacgg
     7681 atcaagcggg aatgagtcca ctccggtatc agaaatggct acgtctcaat gaagtcaggc
     7741 gaccgatgct gaatgaacat tacgatgtca ccacagcagc ctatgctgtc ggttacgaaa
     7801 gctatcccat ttcagtcggg aatattcgcg gatgtttgga gagtcaccca agagagatat
     7861 taccaggtta agaaagtctg tcggtcagct ttaataaacc ctgcacttat ctgtttagag
     7921 caattcggtg ttagtttcag caagcaaaca ttaaccatag ctaatgattt atagccatat
     7981 taaccattgg tagcccttga cctttcctga cacttccctt gttcgcaata atattctgtt
     8041 tagtcacggt ttataaataa catgcataat gaaatcgtca tgttatttag cgacataaaa
     8101 tgtttttgca aatactctct tcaacaagtg cacaataaac ttgttgtgat taggtgtcct
     8161 tgtgatttaa agatattatc gttcttcgtg aaatgtaata accctggaaa ctatattctc
     8221 agggcaacaa ccaatcaaga atgattcttg tttttaagca ataacagaat gacttaattg
     8281 ttaactctct ttattcagct acttaaatat aaattttgga gaattatgaa actcaaacat
     8341 gttggtatga ttgtcgtttc tgtgttggcg atgtcgtctg ctgcggtaag cgcagccgag
     8401 ggtgatgaat cagtaacgac cactgttaat ggcggtgtta ttcattttaa aggtgaagtg
     8461 gtaaatgccg cttgtgcaat tgattcagaa tcaatgaacc aaacggttga gctgggtcag
     8521 gttcgttctt ctcgcctggc taaagcgggt gacctcagct ccgccgttgg cttcaatatc
     8581 aagctgaatg attgtgatac caatgtttcc agtaatgcag ctgttgcatt cctgggtact
     8641 actgtcacca gtaatgacga tacgttagcg ctgcagagtt cagcggcagg ctctgcccaa
     8701 aatgtcggta ttcaaatttt ggaccgtacg ggtgaggtat taatacttga tggggccact
     8761 tttagtgcta aaaccgactt gattgatggc acgaatatac tgccattcca ggctcgttat
     8821 attgctctcg ggcagtccgt agctggtact gcaaacgctg atgcgacctt caaagttcaa
     8881 tatctataat tcaatcttaa ggtggctata cgtcatctga aattgaaaca gggtgtgtgt
     8941 ggcatggcta cgcacacccg agcaatttac caatattcat ctcattttta atgagaaatg
     9001 gtttgtattt ggagcgctat gaaaaatcac ataatatcta aactatcaat aaacaacagt
     9061 agttaaactt actgttattt aaaatattta ctttgtagca aggttttcct attaaaagag
     9121 aaccaacatt tattacaaac gcattaagtc tggaaacccg gcgactcgcc aatgtattag
     9181 caacattaat cgattgtctg atgtaacggt tttcccgctt atcctcttaa cacatggaga
     9241 atgcatcaga tgcaaactac gcgtaccccc tactctattt cctttatggc gacagtgtta
     9301 cttttattgc tctttgcttg ccatagcacc gttgccaacg cagctgtcgc actgggtgcg
     9361 acgcgggtaa tttatccggc aaaccagaaa caggttttat tgcctgtcac gaataatgat
     9421 cctgccagtg tctatctcat tcagtcatgg atagaaaatg caggtgacca aaaagacacc
     9481 cagtttgtca ttaccccacc gcttttttcc atgcaaggta aaaaagagaa tacgctgcgg
     9541 ataatcaacg ctactaatca tcagttacca ggcgaccgtg aaagcctgtt ctgggtgaat
     9601 gttaaagcta ttcctgctat ggaaaaagac cagaagaatg agaatacatt acagctggca
     9661 attattagcc gcataaaaat gttttatcgc cccacgaacc tggcaatggc acctgaagaa
     9721 gcgcctgcaa tgcttcggtt tcgtcgcagc ggtagcaaac tcacgcttat taatccaacg
     9781 ccctatttca ttaccgtaac aaatatgaaa gcaggaaata gcaatctccc aaacaccatg
     9841 gtgccgccaa aaggtgaagt atcggtggat atcacgcatg ctgcaaccgg ggatatcagt
     9901 tttcagacga tcaatgatta cggtgcatta actccgcgta ttaaggcaac catgcagtaa
     9961 gccagtgacc taattaataa taaactcaat cggaattaat aatgacagcc attcgtgccg
    10021 catttaaggc atatcgtatg catcaggtgt taataatgcc tcgctttgca cggcttacca
    10081 ttgcccttgg cttagcaact gcggtatttc ccgttgacgc agagtattat tttaatccac
    10141 ggtttttaag caatgatctt gctgaatctg tcgatttatc cgcctttact aagggacgcg
    10201 aggcaccacc tggtacatat cgggtcgaca tttatttgaa cgatgaattt atgactagtc
    10261 gggatattac ttttattgca gatgataata atgcagaact catcccatgc ctgagtacag
    10321 atcttcttgt cagtcttggt ataaaaaaat cagcgttatt agacaataaa gaacattcgg
    10381 cagaaaaaca tgtgccggac aacagcgcat gcacaccact tcaggatcgt ttggccgacg
    10441 catcaactga gtttgatgtt ggtcagcaac atttgtcact ctccgttccg caaatttatg
    10501 ttggcagaat ggctcgcggc tatgtttctc ctgatctgtg ggaagaagga ataaatgctg
    10561 ggcttctaaa ctatagtttt aatggtaatt ctattaataa tcgtagtaac cataatgcag
    10621 gaaaatcaaa ctatgcatat ttgaatttac agagtggcat caacattggt agttggcgac
    10681 tacgcgataa ctcaacgtgg agttataaca gtgggagtag caattcatct gacagcaata
    10741 aatggcagca tatcaatacg tcggctgaac gtgacattat tcccttacgc tcacggttaa
    10801 cggtaggtga tagttatacc gatggcgata tttttgatag tgtgaacttt cgtggcctca
    10861 aaataaattc aacagaagcg atgttgcccg atagccaaca tggtttcgct cctgtgattc
    10921 atggtattgc ccgtggaacc gcacaagtga gtgtaaaaca gaacggatac gatgtttatc
    10981 agactactgt tccacccggc ccttttacta ttgatgatat caactctgcg accaatggtg
    11041 gtgatctgca agtaaccata aaagaggcag acggcagtat tcagacatta tatgttcctt
    11101 attcgtctgt tccggttctc caacgtgctg gatatacgcg ttatgcgctt gccatggggg
    11161 aatatcgtag tggaaataac ctgcaaagct ctcccaagtt catacaaggt agcttgatgc
    11221 atggtctgga aggaaactgg acaccttatg gcggaatgca aattgcagaa gattatcagg
    11281 ccttcaacct tggtattggg aaagatttag gactttttgg tgccttttct ttcgatatca
    11341 cgcaggccaa tacgacactt gcagatggca cccgtcacag tgggcaatcg gttaaatccg
    11401 tctacagcaa atccttctac cagacaggaa ccaatatcca ggtcgcagga tatcgctatt
    11461 ctacgcaagg tttttataac ttatccgaca gtgcctacag tcgaatgagt ggttacaccg
    11521 tcaagcctcc taccggagac accaatgagc agacacaatt tattgattat tttaatctgt
    11581 tctacagtaa gcgtggtcag gaacaaataa gcatctctca gcagcttgga aattacggta
    11641 cgacattttt cagtgccagt cgccaaagtt actggaacac gtcacgcagc gaccagcaaa
    11701 tatcatttgg attaaatgtg ccgtttggtg atattacgac ttcgctgaat tacagctatt
    11761 ccaataatat atggcaaaac gatcgggatc atttactcgc ttttacgctt aatgttccct
    11821 tcagtcattg gatgcgtaca gacagtcagt cggcatttcg taattcaaac gccagttaca
    11881 gtatgtcaaa cgatttgaaa ggcggcatga ccaatctatc gggggtttat ggcactctgc
    11941 tgccggataa taacctgaat tatagcgttc aggtcggtaa cacccacgga ggtaatacat
    12001 cgtctggcac cagtggttac agttctctta attatcgtgg agcttacggt aatacgaatg
    12061 tcggttacag tcggaatggt gacagcagcc agatttatta cggaatgagt ggtgggatta
    12121 ttgctcatgc tgatggcatc acctttggac agccgctggg cgacacaatg gttctggtta
    12181 aggctcctgg cgctgataat gtcaaaatag agaaccagac cggaattcat accgactggc
    12241 gtggctatgc aatattacca tttgcgacag aatatagaga aaatcgtgtc gctcttaacg
    12301 cgaattccct tgcagataat gttgaactgg atgaaaccgt agtcactgtc atcccaactc
    12361 acggtgctat tgccagagca acatttaatg cacaaatcgg cgggaaagta ttaatgacgt
    12421 tgaagtacgg taataaaagc gttccattcg gtgcaattgt cactcacgga gagaataaaa
    12481 atggcagcat tgtcgcggaa aatggtcagg tttatctgac tggacttcca cagtcaggga
    12541 aattacaggt ttcatggggc aatgataaaa actcaaactg tattgtcgat tacaagcttc
    12601 ctgaagtctc tcctggaacc ttgctgaacc agcagacagc aatctgtcgc taaggaacaa
    12661 tagattatga aatacaataa cgttattttc ctcggtttat gtctggggtt aaccacctat
    12721 tctgctttat ccgcagatag cgttattaaa attagcgggc gcgtcctcga ttatggctgc
    12781 acagtctcat cggattcgct taattttacc gtagatctcc aaaaaaacag tgccagacaa
    12841 tttccaacga ccggtagcac aagtccagcc gtcccttttc agattacgtt aagtgaatgc
    12901 agcaaaggga caacgggggt tcgggttgca tttaacggta ttgaggatgc agaaaataat
    12961 actctgttga aactggatga gggaagcaat acggcctccg gtttaggtat agaaatactg
    13021 gacggaaata tgcgtccggt gaaactgaat gaccttcatg ccgggatgca gtggatccca
    13081 ctggtaccag aacagaacaa tattttgcct tactccgctc gtctgaagtc aactcagaag
    13141 tccgtcaatc cgggactggt gagggcttcg gcaaccttta cccttgaatt tcaataatca
    13201 gagtcagata tgaaaagact tcacaagagg ttcctgttag ctacgttttg cgcgttattc
    13261 acagcaactc tccaggccgc cgatgtcact atcactgtta atggtcgggt agtcgctaaa
    13321 ccctgcacta ttcaaaccaa agaagctaac gttaatctcg gggatcttta tacgcgcaat
    13381 ctgcaacaac ctggttctgc atctggctgg cacaatatta ctttgtcatt aaccgattgt
    13441 ccggttgaaa caagtgcagt gacggcaatc gtgacaggtt caactgacaa tacgggttat
    13501 tacaaaaatg aaggtactgc cgaaaatatt cagatagagc tgagggatga ccaggatgcg
    13561 acgttaaaaa atggcgatag caaaacggtt attgttgatg agatcactcg taatgcacag
    13621 tttccactta aggcaagagc tatcacggtg aatggaaacg caagccaggg aacgatcgag
    13681 gcgctaatca atgtgatcta cacctggcaa taacgtcgat tcgattttct tagccttctt
    13741 tttaaatcaa ttttttaacc ttaggatata tcatgggtaa aacaatcagt ataaaggtcc
    13801 tgttcggtat ttatcttcta ttaatggcag gtaaagtttt tgccttcagt tgtaatgtcg
    13861 atggcgggtc aagtattgga gctggtacaa cctctgttta tgttaatctc gaccctgtaa
    13921 tacagccggg ccagaatctg gttgtagact tgtctcagca tataagttgc tggaatgatt
    13981 acggcggctg gtacgacact gatcatataa acctggtaca aggctcagct ttcgcaggct
    14041 ctctacagtc atataaagga tcgctgtact ggaataatgt gacctacccc ttcccgttga
    14101 cgacaaatac caacgtattg gatattggtg ataagacacc gatgccatta ccgttaaaac
    14161 tctatattac cccagtcggg gcggccggtg gagttgtcat aaaagccggg gaagttattg
    14221 cccgtattca tatgtataaa attgcaacat taggaagcgg aaatccgcgt aattttacgt
    14281 ggaatattat ttcaaataac agtgttgtta tgccaacagg cggctgcact gttgatagtc
    14341 gtaatgtcac cgtggatctc ccggactttc ctggaagcgc agaaataccc cttggcgtat
    14401 attgctcaag cgaacaaaag ctctcatttt atctttcggg tgccaccact gacagtgccc
    14461 gacaagtgtt tgctaacaca gcacctgatg caacaaaagc atcgggagtg ggtgtctctc
    14521 tgatgcgtaa tggaaaaata ctggctacgg gagaaaatgt ctccctggga acagtaaata
    14581 aaagcaaagt acctctgggt ttaagcgcaa cctatggaca aacaggaaac aaggtttcag
    14641 ccggaaccgt ccagtcagtc attggtgtca cttttatcta tgaataattg tgattttgat
    14701 cttagtctat gcgctccatt cacgaaaatt gcgcggat
//
LOCUS       DBANUU010000108        14623 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000108 DBANUU010000000
VERSION     DBANUU010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.108"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(29..1483)
                     /gene="xylB"
                     /locus_tag="ACIE68_003413"
     CDS             complement(29..1483)
                     /gene="xylB"
                     /locus_tag="ACIE68_003413"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFX5909676.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLHLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="ACIE68_003414"
     CDS             complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="ACIE68_003414"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFX5909677.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGVFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3243..4235
                     /gene="xylF"
                     /locus_tag="ACIE68_003415"
     CDS             3243..4235
                     /gene="xylF"
                     /locus_tag="ACIE68_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFX5909678.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            4313..5854
                     /gene="xylG"
                     /locus_tag="ACIE68_003416"
     CDS             4313..5854
                     /gene="xylG"
                     /locus_tag="ACIE68_003416"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFX5909679.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5832..7013
                     /gene="xylH"
                     /locus_tag="ACIE68_003417"
     CDS             5832..7013
                     /gene="xylH"
                     /locus_tag="ACIE68_003417"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFX5909680.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7091..8269
                     /gene="xylR"
                     /locus_tag="ACIE68_003418"
     CDS             7091..8269
                     /gene="xylR"
                     /locus_tag="ACIE68_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFX5909681.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARINKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8377..9201)
                     /locus_tag="ACIE68_003419"
     CDS             complement(8377..9201)
                     /locus_tag="ACIE68_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFX5909682.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9521..11551
                     /gene="malS"
                     /locus_tag="ACIE68_003420"
     CDS             9521..11551
                     /gene="malS"
                     /locus_tag="ACIE68_003420"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFX5909683.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11729..12982
                     /gene="avtA"
                     /locus_tag="ACIE68_003421"
     CDS             11729..12982
                     /gene="avtA"
                     /locus_tag="ACIE68_003421"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFX5909684.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13133..13606)
                     /locus_tag="ACIE68_003422"
     CDS             complement(13133..13606)
                     /locus_tag="ACIE68_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFX5909685.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVNAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13708..14556)
                     /gene="yiaJ"
                     /locus_tag="ACIE68_003423"
     CDS             complement(13708..14556)
                     /gene="yiaJ"
                     /locus_tag="ACIE68_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFX5909686.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
BASE COUNT         3698 a         3732 c         3598 g         3595 t
ORIGIN      
        1 ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt tgctgataga
       61 ggcgacggaa cgtttcacgt cgtggctgat aagcggcata acgctgcgca tctggtagat
      121 gcgactgttc taacggtagc tgcggcaaca actcaatgag cgatttctct ggattcgccg
      181 caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc gtacggtaat
      241 cgagctgctg accgctgata tccgccagca tctgacgcca gtactcacta cgcgccccgc
      301 caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc atgcacgaca tccatgccat
      361 ctgccagcgc ataacccacg ccttccagca ctgctcgcgc cagttcattg gggccatgtt
      421 gatgagtcaa accaaagaaa acccccttcg cctggggatt attgtgtggt gtacgctcgc
      481 cggaaagata aggcagaaac caaactggct cggcactttc atcagcctgt tgagctgcag
      541 cgattaaagc tgggacattg cacaggccgg ttaatttcgc gacccaatcc agacacgatg
      601 ctgcactcag cattacagac attaaatgcc aacgctgcgg tagcgcatgg caaaagctgt
      661 gtacagcgct ttctggcttg cttaagaacc cttcgctgac agcaaaatag acccccgacg
      721 tccccagcga taacattgcc tgattagcat caaccattcc cacaccaact gcaccagctg
      781 cattgtcgcc accgcctgcg acaactggca ccgtcgccat accccacgct ttcgcaactt
      841 caggtaacaa agcaccagta atttcgctgc cttcgtataa tgcgggcatc tggtcacgag
      901 ataagtcgca agcctgcagc atgacgtcac tccagtcacg ctttgcgaca tccagccaca
      961 tggtgccagc tgcgtcagac atatcgctgg caaactcccc cgtcatacgc agatgcaagt
     1021 aatctttcgg taataatact ttgtcgattt gacggaatat ctccggctca tgccgctgaa
     1081 cccatagcaa tttaggcgca gtaaatccgg gcatcatcag gttgccggta atcactcgtg
     1141 attgcggaac tctcgcttcc agcaaagtgc actcttgcgc acagcgcccg tcgttccaca
     1201 aaatggcagg gcgcaatacc cgttgttgag catccagtaa ggttgctcca tgcatctggc
     1261 cggcaatacc caatgcttta acgtcctgca gagaatgctg atcgcccaga gctttcattg
     1321 cgcgatcagt tgcctgccac cactgttccg ggtcttgttc cgaccagagt ggatgcgggc
     1381 gcgaaacggt cagcttttcc gtttgcgaag caaccacctc accctgctcg ttgagcaaaa
     1441 taacttttac gcccgaggtg ccaagatcta tcccgatata catatcgatc gttccttaaa
     1501 aaaatgcccg gtatcgctac cgataaccgg gccaacggac tgcacagtta gccgttattt
     1561 gtcgaacaga taatgattta ccagattttc cagttgctcc tggcgaccac tctgatgcac
     1621 cggagacaaa ttatgttcct gagcatattt ggctaaatct gccagtgaca tttggccttt
     1681 caggatttgc tggcccaatt cgctattcca gccggaataa cgctgcgcga tgcgtttatc
     1741 cagctcgcca tcttcaatca tgcgcgctgc aattttcagc gccagtgcca tcgtatccat
     1801 cgcgccgata tgaccgtaaa acagatcata tttatcagta ctttgacgac gtactttggc
     1861 atcgaagttc agaccaccgg tggtgaaacc gcctgctttg agaatttcat acatcaccag
     1921 cgcattctct tccacactgt tcgggaactg gtcggtgtcc cagcccagtt gcgcatcgcc
     1981 acggttggcg tcgacagaac cgaacaggcc aagcgcaatg gcggtggcta tttcatgatg
     2041 gaaagagtga cctgccagcg tcgcgtggtt agcttcaatg ttcagtttaa tctctttttc
     2101 cagaccaaac tgtttcagga agccatagac cgtcgcggca tcgtaatcat attgatgttt
     2161 agtcggttct tgcggtttcg gttcgataag caacgtgccc tggaagccga ttttatgttt
     2221 atgctcaacc accatctgca taaagcggcc cagttgttca cgctcctgac gcaagtcggt
     2281 atttaacagc gtttcgtaac cttcacgacc accccacagg acatagtttt caccgcccaa
     2341 tttatgggtt gcttccatcg ctgtaacaac ttgcgttgcc gcccagctga agacttcagg
     2401 atctgggttc gtcgccgcac ccgcgccgta gcgagggttt gtaaagcagt tggcggttcc
     2461 ccacagcagc ttcacgccgc tctcttcttg cttgcctgcc aggacatcaa ccatttgcgc
     2521 aaaattattg atgtactctt ttaacgacgc gccctcaggg gaaacatcca catcgtggaa
     2581 gcaataaaat ggcacatgta acttgtggaa aaactcaaat gcgacatctg ctttacgctt
     2641 cgccaacgcc agtgcctcac caggctgctg ccacggacga ttaaacaccc ccacaccaaa
     2701 catgtccgcc ccgttccagc agaaggtgtg ccagtagcag gcggcaaaac gcaagtgctc
     2761 ttccatacgc ttacccaaca ccagttcgtc gggattgtag tgacggaatg ctaacgggtt
     2821 tgaggatttt gagccttcat aacgaacgcg atcgagctgg tcaaaatagg cttgcatatt
     2881 gaactccata atcaggtaat gccgcgggtg atggatgatg tcgtaatatt gggcactccc
     2941 tttcagttgc tcaattatgt tatttcacac tgctattgag ataattcaca agtgtgcgct
     3001 cgctcgcaaa ataaaatggg atgatgaaac tgggtaattc ctcgaagata aaaatgcaat
     3061 aagtacaatt gcgcaacaaa agtaagatct cggtcataaa tcaagaaata aaccaaaaat
     3121 cgtaatcgaa agataaaaat ctgtaattgt tttcccctgt ttagttgcta aaaattggtt
     3181 acgtttatcg cggtgattgt tacttattaa agctgtcctc taactacaga aggccctaca
     3241 ccatgaaaat aaagaacatt ctactcaccc tttgcacctc acttctgctt accaacgttg
     3301 cggcacacgc caaagaagtc aaaataggta tggcgattga tgatctccgt cttgaacgct
     3361 ggcaaaaaga tcgagatatt tttgtgaaaa aggcagaatc tctcggcgcg aaagtatttg
     3421 tacagtctgc aaatggcaat gaagaaacac aaatgtcgca gattgaaaac atgattaacc
     3481 ggggtgtcga tgttcttgtc attattccgt ataacggcca ggtattaagt aacgttgtaa
     3541 aagaagccaa acaagaaggt attaaagtat tagcttacga ccgtatgatt aacgatgcgg
     3601 atatcgattt ttatatttct ttcgataacg aaaaagtcgg tgaactgcag gcaaaagccc
     3661 tggtcgatat tgttccgcaa ggtaattact tcctgatggg cggctcgccg gtagataaca
     3721 acgccaagct gttccgcgcc ggacaaatga aagtgttaaa accttacgtt gattccggaa
     3781 aaattaaagt cgttggtgac caatgggttg atggctggtt accggaaaac gcattgaaaa
     3841 ttatggaaaa cgcgctaacc gccaataata acaaaattga tgctgtagtt gcctcaaacg
     3901 atgccaccgc aggtggggca attcaggcat taagcgcgca aggtttatca gggaaagtag
     3961 caatttccgg ccaggatgcg gatctcgcag gtattaaacg tattgctgcc ggtacgcaaa
     4021 ctatgacggt gtataaacct attacattgc tggcaaatac tgccgcagaa attgccgttg
     4081 aattgggcaa tggtcaggag ccaaaagcag atacctcact gaataatggc ctgaaagatg
     4141 tcccctcccg cctcctgaca ccgatcgatg tgaataaaaa caacatcaaa gatacggtaa
     4201 ttaaagacgg attccacaaa gagagcgagc tgtaagcgtt acgccccggc gcggagcggg
     4261 ggcgtgattt ctctccatgc cgcgtgaatg aattggctta ggtggagtcg ttatgcctta
     4321 tctacttgaa atgaagaaca ttaccaaaac cttcggcagt gtgaaggcga ttgataacgt
     4381 cagcttgcgg ttgaatgctg gcgaaatcgt ctcactctgt ggggaaaatg ggtctggtaa
     4441 atcaacgctg atgaaagtgc tgtgtggtat ttatccccat ggctcctacg aaggcgaaat
     4501 tatttttgcg ggagaagaga ttcaggcgag tcacatccgc gataccgaac gcaaaggtat
     4561 cgccatcatt catcaggaat tggccctggt gaaagaattg accgtgctgg aaaatatctt
     4621 cctgggtaac gaaataaccc acaatggcat tatggattat gacctgatga cgctacgctg
     4681 tcagaagctg ctcgcacagg tcagtttatc catttcacct gatacccgcg ttggcgattt
     4741 agggcttggg caacaacaac tggttgaaat tgccaaggca cttaataaac aggtgcgctt
     4801 gttaattctc gatgaaccga cagcctcatt aactgagcag gaaacgtcgg ttttactgga
     4861 tattattcgc gatctacaac agcacggtat cgcctgtatt tatatttcgc acaaactcaa
     4921 cgaagtcaaa gcgatttccg atacgatttg cgttattcgc gacggtcagc acattggtac
     4981 gcgtgatgct gccggaatga gtgaagacga tattatcacc atgatggtcg ggcgagagtt
     5041 aaccgcgctt taccctaatg aaccacatac caccggagat gaaatattac gtattgaaca
     5101 tctgacggca tggcatccgg ttaatcgtca tattaaacga gttaatgatg tctcgttttc
     5161 cctgaaacgt ggcgaaatac tgggtattgc cggactcgtt ggtgccggac gtaccgagac
     5221 cattcagtgc ctgtttggcg tgtggcccgg acaatgggaa ggaaaaattt atattgatgg
     5281 caaacaggta gatattcgta actgtcagca agccatcgcc caggggattg cgatggtacc
     5341 cgaagacaga aagcgcgacg gcatcgttcc ggtaatggcg gttggtaaaa atattaccct
     5401 cgccgcactc aataaattta ccggtggcat tagccagctt gatgacgcgg cagagcaaaa
     5461 atgtattctg gaatcaattc agcaactcaa agttaaaacg tcgtcccccg accttgctat
     5521 tggacgtttg agcggcggca atcagcaaaa agcgatcctc gctcgctgtc tgttacttaa
     5581 cccgcgcatc ctcattcttg atgaacctac caggggtatc gatattggcg cgaaatacga
     5641 gatctacaaa ttaattaacc aactcgtcca gcagggtatt gccgttattg tcatctcttc
     5701 cgaattacct gaagtgctcg gccttagcga tcgggtactg gtgatgcatg aagggaaact
     5761 aaaagccaac ctgataaatc ataacctgac tcaggagcag gtgatggaag ccgcattgag
     5821 gagcgaacat catgtcgaaa agcaatccgt ctgaagtgaa attggccgta ccgacatccg
     5881 gtggtttttc tgggctgaag tcactgaatt tgcaggtctt cgtgatgatt gcagctatca
     5941 tcgcaatcat gctgttcttt acctggacca ccgatggtgc ctacttaagc gcccgtaacg
     6001 tctccaacct gttacgccag accgcgatta ccggcatcct cgcggtagga atggtgttcg
     6061 tcataatttc tgctgaaatc gacctttccg tcggctcaat gatggggcta ttaggtggcg
     6121 tcgcggcgat ttgtgacgtc tggttaggct ggcctttgcc acttaccatc attgtgacgt
     6181 tggttctggg actgcttctc ggtgcctgga acggatggtg ggtcgcgtac cgcaaagtcc
     6241 cttcatttat tgtcaccctc gcgggcatgt tggcatttcg cggcatactc attggcatca
     6301 ccaacggcac gactgtttcc cccaccagcg ccgcgatgtc acaaattggg caaagctatc
     6361 tacctgccag cactggcttc atcattggcg cgcttggctt aatggctttt gttggttggc
     6421 aatggcgcgg aagaatgcgc cgtcaggctt tgggtttgca gtctccggcc tctaccgcag
     6481 tagtcggtcg ccaggcttta accgctatca tcgtattagg cgcaatctgg ctgttgaatg
     6541 attaccgtgg cgttcccact cctgttctgc tgctgacgtt gctgttactc ggcggaatgt
     6601 ttatggcaac gcggacggca tttggacgac gcatttatgc catcggcggc aatctggaag
     6661 cagcacgtct ctccgggatt aacgttgaac gcaccaaact tgccgtgttc gcgattaacg
     6721 gattaatggt agccatcgcc ggattaatcc ttagttctcg acttggcgct ggttcacctt
     6781 ctgcgggaaa tatcgccgaa ctggacgcaa ttgcagcatg cgtgattggc ggcaccagcc
     6841 tggctggcgg agtgggaagc gttgccggag cagtaatggg ggcatttatc atggcttcac
     6901 tggataacgg catgagtatg atggatgtac cgaccttctg gcagtatatc gttaaaggtg
     6961 cgattctgtt gctggcagta tggatggact ccgcaaccaa acgccgttct tgattttgat
     7021 aaaaattttc tcaaagccgg ttacgtatta ccggttttga gtttttgcat gattcagcag
     7081 gaaaagaacc atgtttacta aacgtcaccg catcacatta ctgttcaatg ccaataaagc
     7141 ctatgaccgg caggtagtag aaggcgtagg ggaatattta caggcgtcac aatcggaatg
     7201 ggatattttc attgaagaag atttccgcgc ccgtattaat aaaatcaagg actggttagg
     7261 agatggcgtc attgccgact tcgacgacaa acagatcgag caagcgctgg ctgatgtcga
     7321 cgtccccatt gttggggttg gcggctcgta tcaccttgca gaaagttacc cacccgttca
     7381 ttacattgcc accgataact atgcgctggt tgaaagcgca tttttgcatt taaaagagaa
     7441 aggcgttaac cgctttgctt tttatggtct tccggaatca agcggcaaac gttgggccac
     7501 tgagcgcgaa tatgcatttc gtcagcttgt cgctgaagaa aagtatcgcg gagtggttta
     7561 tcaggggtta gaaaccgcgc cagagaactg gcaacacgcg caaaatcggc tggcagactg
     7621 gctacaaacg ctgccaccgc aaaccgggat tattgccgtt actgacgccc gagcgcggca
     7681 tattctgcaa gtatgtgaac atctacatat tcccgtaccg gaaaaattat gcgtgattgg
     7741 catcgataac gaagaactga cccgctatct gtcgcgtgtc gccctttctt cggtcgctca
     7801 gggcgcgcgg caaatgggct atcaggcggc aaaactgttg catcgattat tagataaaga
     7861 agaaatgccg ctacagcgaa ttttggtccc accagttcgc gtcattgaac ggcgctcaac
     7921 agattatcgc tcgctgaccg atcccgccgt tattcaggcc atgcattaca ttcgtaatca
     7981 cgcctgtaaa gggattaaag tggatcaggt actggatgcg gtcgggatct cgcgctccaa
     8041 tcttgagaag cgttttaaag aagaggtggg tgaaaccatc catgccatga ttcatgccga
     8101 gaagctggag aaagcgcgca gtctgctgat ttcaaccacc ttgtcgatca atgagatatc
     8161 gcaaatgtgc ggttatccat cgctgcaata tttctactct gtttttaaaa aagcatatga
     8221 cacaacgcca aaagagtatc gcgatgtaaa tagcgaggtc atgttgtagc gatgatgaga
     8281 attgtcggcg tcacatcagg gaactcagca cagccacctg atgcgagcaa aagccttatc
     8341 cgaccgacaa atcagataag gctcaaggaa atgcaattac atatgcgccg cgattaaccg
     8401 ttggttatcc tggtacattg cgaacaggta gttattataa ctcttccctt tggtcgagta
     8461 gcccttcagc ttgtgaatca tcgctgtggc agtcacttcc tgatccgctt tacgcagctg
     8521 cgcacgcgat ttacggaacg aagagtaagc cgggtgcgta ttcaggttag tgacataggc
     8581 gctcaccgat tctttgacag aactaaactg tgagtaccct ttcactttac ccggcgcatt
     8641 ggtacaacgt cctttcatgc atttcatgcc gaacagattg ttattgttgc gcgccagctt
     8701 cgacgttccc caaccgcttt ctgctgcagc catcgtcgcc accatactgg tagggataat
     8761 gtctacgcgt tcaagcaagg tattccacgg gatttttcgc gtattaccag accacttcac
     8821 cttgtagcgt ttggcgatgt ctttcagacg cgcacgctca gcaggtgacc attggccctg
     8881 atactgtttt gaaatcagcc agttacgttc tgcagtaatg gctgcatttt ggctggtaat
     8941 gtaaggcatt acggtccgga gaaacgcctt tttccttggt gttccggaag ggtattttcg
     9001 caaatcagga agtgaactac tctttgcact attgcgagaa tactcttgtt tactgcttac
     9061 ctgtttatta ctagctttag ttaagtggga cttttgactc gctgttgttg tgtgcgtctt
     9121 cgctaacacc tcactcgaaa acaccagagt gagtaacata agaatcatcg ccccatatcg
     9181 tcgtatggga gtcaaaatca tcaggtctcc tggtcggatt taatcattcc aacaccttat
     9241 atttttcaca aatttgagag ttgaatctca aatcatatca aaaatagctg tcaagagcac
     9301 cccaaggaat agtccaaatc tgaaactatg tcacgtgtta acgattcaga ttggcgctaa
     9361 atcgcagaaa atgtggggtt tatcgcaaaa ttcagccgtt ttttgcgcga gatcgctcac
     9421 ccttgcttct catcctgtgg acttaccgct cagggatgag ttttgtctgg cttatcgctg
     9481 gcaaactgtc tgaaatcgca gcaataagga ctcatccacc atgaaactcg ccgcctgttt
     9541 tctgacactc cttcctggct tcgccgttgc cgccagctgg acttctccgg ggttccctgc
     9601 ctttagcgaa cagggaacgg gaacatttgt cagccacgcg cagttgccca aaggtacgcg
     9661 tccactcacg ctaaattttg accaacagtg ctggcagcct gcagatgcga taaaactcaa
     9721 tcagatgctt tccctgcaac cttgtagcaa cacgccgcct caatggcgat tgttcagaga
     9781 cggcaaatat acgctgcaaa tagacacccg ctccggtacg ccaacattga tgatttccat
     9841 ccagaacgcc gccgaaccgg tagcaaacct ggtccgtgaa tgcccgaaat gggatggatt
     9901 accgctcacg ctggatgtca gcgccacttt cccggaagga gccgccgtac gggattatta
     9961 cagccagcaa attgcgatag tgaagaacgg tcaaataacg ttacaacccg ctgctaccag
    10021 caacggttta ctcctgctgg aacgggcaga aactgacgcc cctgcccctt tcgactggca
    10081 taacgccacg gtttactttg tgctgacaga tcgtttcgaa aacggcgatc ccagtaatga
    10141 ccagagttac ggacgtcata aagacggtat ggcggaaatt ggcacttttc acggcggcga
    10201 tttacgcggc ctgaccaaca aactggatta cctccagcag ttgggcgtta atgctttatg
    10261 gataagcgcc ccatttgagc aaattcacgg ctgggtcggc ggcggtacaa aaggcgattt
    10321 cccgcattat gcctaccacg gttattacac acaggactgg acgaatcttg atgccaatat
    10381 gggcaacgaa gccgatctac ggacgctggt tgatagcgca catcagcgcg gtattcgtat
    10441 tctctttgat gtcgtgatga accacaccgg ctatgccacg ctggcggata tgcaggagta
    10501 tcagtttggc gcgttatatc tttctggtga cgaggtgaaa aaaacgctgg gtgaacgctg
    10561 gagcgactgg aaacctgccg ccgggcaaac ctggcatagc tttaacgatt acattaattt
    10621 cagcgacaaa acaggctggg ataaatggtg gggaaaaaac tggatcagaa cggatatcgg
    10681 cgattacgac aatcctggat tcgacgatct cactatgtcg ctggcctttt tgccggatat
    10741 caaaaccgaa tcaactaccg cttctggtct gccggtgttc tataaaaaca aaacggatac
    10801 tcacgctaaa gtcatcgaag gctttacacc tcgcgattac ttaacccact ggttaagtca
    10861 gtgggtccgc gactatggga ttgatggttt tcgggtcgat accgccaaac atgttgagtt
    10921 gcccgcctgg cagcaactga aaaccgaagc cagcgccgcg cttcgcgaat ggaaaaaagc
    10981 taaccccgac aaagcattag atgacaaacc tttctggatg accggtgaag cctggggcca
    11041 cggcgtgatg caaagtgact actatcgcca cggcttcgat gcgatgatca atttcgatta
    11101 tcaggagcag gcggcgaaag cagtcgattg tctggcgcag atggatacga cctggcagca
    11161 aatggcggag aaattgcagg gtttcaacgt gttgagctac ctctcgtcgc atgatacccg
    11221 tctgttccgt gaagggggcg acaaagcagc agagttatta ctattagcgc caggcgcggt
    11281 acaaatcttt tatggcgatg aatcctcgcg tccgttcggt cctacaggtt ctgatccgct
    11341 gcaaggtaca cgttcggata tgaactggca ggatgttagc ggtaaatctg ccgccaacgt
    11401 cgcgcactgg cagaaaatca gccagttccg cgcccgccat cccgcaattg gcgcgggcaa
    11461 acaaacgaca ctttcgctga agcagggcta cggctttgtt cgtgagcatg gcgacgataa
    11521 agtgctggtc atctgggctg ggcaacagtg acttttccgg cttcccgttc gtcagtacct
    11581 cgggaagccg ccaaccagga taaaatgtca gccctaatca gcgttgcagg ataaagcacc
    11641 gctcactctc caacagaccg atttgcaccc cagcaaatgt agcgttattg ttaccttcct
    11701 tgctacagag ttcgacagat atcccgctat gacattctcc ctttttggtg acaaatttac
    11761 ccgccactcc ggcattacgc tgctgatgga agatctgaac gacggtttac gcacgcctgg
    11821 cgcgattatg ctcggcggcg gtaatccggc gcagatcccg gaaatgcagg actacttcca
    11881 gacgctactg accgacatgc tggaaagtgg caaagcgact gatgcactgt gcaattacga
    11941 cggtccacag gggaaaacgg agctactcac actgcttgcc ggaatgctgc gcgagaagtt
    12001 gggttgggat atcgaagcac agaatattgc actaacaaac ggcagccaga gcgcgttttt
    12061 ctacttattt aatctgtttg ccggacgccg tgccgatggt cgggtcaaaa aagtgctgtt
    12121 cccgctggca ccggaataca ttggctacgc tgacgccgga ctggaagaag atctgtttgt
    12181 ctctgcgcgt ccgaatattg aactgctgcc ggaaggccag tttaaatacc acgtcgattt
    12241 tgagcatctg catattggcg aagaaaccgg gatgatttgc gtctcccggc cgacgaatcc
    12301 aacaggcaat gtgattactg acgaagagtt gctgaagctt gacgcgctgg cgaatcaaca
    12361 cggcattccg ctggtgattg ataacgctta tggcgtcccg ttcccgggta tcatcttcag
    12421 tgaagcgcgc ccgctatgga atccgaatat cgtgctgtgc atgagtcttt ccaagctggg
    12481 gctgcctggc tcccgctgcg gcattatcat cgccaatgaa aaaatcatca ctgccatcac
    12541 caatatgaac ggcattatca gcctggcacc tggcggtatt ggtccggcga tgatgtgtga
    12601 aatgattaag cgtaacgatc tgctgcgcct gtctgaaaca gtcatcaaac cgttttacta
    12661 ccagcgtgtt caggaaacta tcgccatcat tcgccgctat ttaccggaag atcgctgcct
    12721 aattcataaa ccagaaggag ccattttcct ctggctatgg tttaaggatt tgcccattac
    12781 gaccgagcaa ctctaccagc gcctgaaagc acgcggcgtg ctgatggtgc cggggcacaa
    12841 cttcttcccg ggactggata aaccgtggcc gcatacgcat caatgtatgc gcatgaacta
    12901 cgtaccagag ccggagaaaa ttgaggcggg ggtgaagatt ctggcggaag agatagaaag
    12961 agcctgggct gaaagtcact aaaaattgcc tgatgcgcta cgcttatcag gcctacataa
    13021 tttctgcaat atattgaatt tggaagaatt tgtaggccgg ataaggcgtt tacgccgcat
    13081 ctggcataaa caaagcgcac tattccggcg ttgagaaacg ccggaaaacg tttcaaacag
    13141 gctgctgccg tagccgatgc cgctgcactt tctcgacatc catgcactgc aacgcctttg
    13201 tcgggcaggc ttcaacacag gccggccccg tctcccgatg ccagcacaga tcgcacttaa
    13261 tcgccctcgc ctttttgcgc gaagaaacga cctccatcgc accaaacggg caagccagca
    13321 tacagctttt acagccaatg caacgtgttt gttcaacgaa aatatgccca tgttcgcggc
    13381 ttatcgcgtt aacagggcag acattcgcgc acggtgcatc ttcacactga tgacaggcta
    13441 ctgccgtggt ccagctgtgg tctttaatga cacgaatacg ggaaataaac tcgtctggtg
    13501 acaacgcagc gcaatcctga ttctcctgat gcgacactgc gcaagccact tcacaggtac
    13561 ggcaaccgat acatttcgtc gcatccgcaa taataaaccg gttcatgatc actcctttgc
    13621 actaaaacag cagagtgcca gagagcgcgt ccctactgct ttgatccaac gaaggaaatg
    13681 ttaaagtttt ggcacttgtc caaagcgtta tgtgatcgcg ccctggtcgt ccctgacagt
    13741 aaatcccagt tcattagaaa tagcctgcgc ggtttcacgc agtggtttca ggagattttt
    13801 ctcccccacc tgtttcagac gtgatgtcga aagcgaaatc gacacggcgt acggcacccg
    13861 cccatgaata ttaaacaccg gaacagcaat acaggagacg ccgagttcgt tttcttctct
    13921 gtccatcgcc gctccgcttt cacgaatgtg cgccagttcg tcgaacatcg cgggcagctc
    13981 ggtaatggta ttgcgggtta acggctggat ctcatgctga tggttttccc agtatgactt
    14041 cacgtagtcc gggtgaccaa acgccatgta gatcttgccc attgcggaac agtagagcgg
    14101 catatgctgg ccaatatagg cacgggttcg cagcatcccg gttgtgggtt ccagcttata
    14161 aatcaaaata gcgtgatcgt cttcgcggct ggagaagtta atggtttcac cagtggcgat
    14221 gttcagtgcc tcaagatgcg gagcggcgat atgaatgata ttcagcgaag acagcgcctt
    14281 ctgcccgacg gcaataaatt tggtagtcag gcggtaactc cctgcggcgg gcgcggtggt
    14341 cacatagccg caggactgta atccctgcaa taagcgatgg acggtactct tatttaaacc
    14401 agccagctcc gaaagatgcg ccaacggaca accgtttgga tagttgctca aaatctcaat
    14461 cagcatcaac ccgcgaaaca gactctggct tccggctgga cgctcttttt cctgcgccat
    14521 ctcgttctct ttttttccca tcacttcttt ccccattttg tcgcgtcctg atggtagcgc
    14581 aaagtgtgcc gtagttcacg atctcgacag ataatttata acc
//
LOCUS       DBANUU010000109        14557 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000109 DBANUU010000000
VERSION     DBANUU010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.109"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            3..872
                     /locus_tag="ACIE68_003424"
     CDS             3..872
                     /locus_tag="ACIE68_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFX5909687.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGNKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLVGMPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1065..1418)
                     /locus_tag="ACIE68_003425"
     CDS             complement(1065..1418)
                     /locus_tag="ACIE68_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFX5909688.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1556..3202)
                     /gene="groL"
                     /locus_tag="ACIE68_003426"
     CDS             complement(1556..3202)
                     /gene="groL"
                     /locus_tag="ACIE68_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFX5909689.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3246..3539)
                     /locus_tag="ACIE68_003427"
     CDS             complement(3246..3539)
                     /locus_tag="ACIE68_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFX5909690.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3815..5071
                     /gene="yjeH"
                     /locus_tag="ACIE68_003428"
     CDS             3815..5071
                     /gene="yjeH"
                     /locus_tag="ACIE68_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFX5909691.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            complement(5087..5563)
                     /locus_tag="ACIE68_003429"
     CDS             complement(5087..5563)
                     /locus_tag="ACIE68_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFX5909692.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            5900..7336
                     /gene="aspA"
                     /locus_tag="ACIE68_003430"
     CDS             5900..7336
                     /gene="aspA"
                     /locus_tag="ACIE68_003430"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFX5909693.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7454..8755
                     /gene="dcuA"
                     /locus_tag="ACIE68_003431"
     CDS             7454..8755
                     /gene="dcuA"
                     /locus_tag="ACIE68_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFX5909694.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFLLGSFML"
     gene            8871..9209
                     /gene="cutA"
                     /locus_tag="ACIE68_003432"
     CDS             8871..9209
                     /gene="cutA"
                     /locus_tag="ACIE68_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFX5909695.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9185..10882
                     /gene="dsbD"
                     /locus_tag="ACIE68_003433"
     CDS             9185..10882
                     /gene="dsbD"
                     /locus_tag="ACIE68_003433"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFX5909696.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10919..11494
                     /locus_tag="ACIE68_003434"
     CDS             10919..11494
                     /locus_tag="ACIE68_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFX5909697.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11601..11676
                     /locus_tag="ACIE68_003435"
     tRNA            11601..11676
                     /locus_tag="ACIE68_003435"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11634..11636,aa:Phe,seq:gaa)
     gene            11874..13139
                     /locus_tag="ACIE68_003436"
     CDS             11874..13139
                     /locus_tag="ACIE68_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFX5909698.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTSVKRQHRPALDLSRLPELLSRINS
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWAEIDIDNSMWTIPAERKPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TKEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREECISPFEYAKVNNPLKR"
     gene            13395..14438
                     /locus_tag="ACIE68_003437"
     CDS             13395..14438
                     /locus_tag="ACIE68_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFX5909699.1"
                     /translation="MTGKLRFEVNDNQGCFIFPETWFGSLLDEFEELIDAYDADEISE
                     TSYINKLRRLARQENDFIDVHAHLAYVFLEQNAPRKALNAALKGLAVGNRLIPEGFSG
                     RIIWIHPDNRPFLRALYAAILANAHLQRHQDAIMLIEKILDYNPEDNHGARWLLGPEL
                     LRTGAHEQARHILQEHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNFSGGPDTAEDYYATYHPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQEKLRERIDETLSEKIVQKCRNMNGEYVWPWILPF
                     SAAGMKHTGIQYQ"
BASE COUNT         3589 a         3701 c         3548 g         3719 t
ORIGIN      
        1 acatggccat aagcatcaag ggtgttaata ccggtgttat tcgcaagagt aataatttca
       61 ttgcattggc gctgaaaatt aaagaacctc gcaataaaga atcgttgttc tttatgtctg
      121 tcatggaact gcgcgacctg ctgattgcgc tggaaagtcg tctgcatcaa aaacataaac
      181 tggatgctgc tgcacgcctt cagtatgagc aagctcgcga taaagtcatt aagaaaatgg
      241 cagaaaacat cccagaaatt ctggttgatg agcttaaaaa tgccgatatt aatcgtcggg
      301 ttaacacgct ggaacttacc gataatcaag gcgaaaatct taccttcgta ttaacgctac
      361 atgacggtaa caaatgtgag ttagtcgtca atgagttgca gattgaaatg ctggcgcgag
      421 caatcattca tgcaattaat aatgcggaaa tgcgtgaact ggcattacgt attacctcac
      481 tgttagattt cctgccgtta tatgacgttg actgccagga aaatggcaat ctggaatatg
      541 acacctattc ccaaccggaa tggaaacata acttgtttga ccattatctg gcggtgcttt
      601 accgctttaa agatgaaagt ggcaaagaac aatttagcgg cgcagtggtg aaaacgcgtg
      661 aagcaacgcc gggtaaagaa gtagaggcga tcactcgccg aatgcttgat ttcagcccac
      721 gactgaaaaa actcgtgggt atgccatgtc aggtctatgt tcgcaccgta gcggcgaata
      781 acgcccaacc gctaacccag gatcaatgct tacgcgcgct gcatcactta cgtgttcaat
      841 ccaccagcaa aactgcacca caggcgaaat aatctcatct ttgctaatgc gaggctggag
      901 ccatccagcc tgaaatgact gataacgtga tcgttggtca tgccggatgc ggcgtaaacg
      961 ccttatccgg cctacaaatt ctcaagcatt caatcagttg caatgcatta tgtaggcctg
     1021 ataagcgaag cgcatctggc aattatacgt ttgtcaccat cacatcagtt cgggcactta
     1081 taaacctggc cgataatctg gctatccgtc gggacaaaac tggacaacat tccctgcgac
     1141 gggctactga tgccataaat cacgttaccg cccattgcag ccgcctggtt gcgcagatcg
     1201 tttgctgcgc cgcgcataga accgccttct tctccgtgtt gcccggaaag ccagttgctt
     1261 tgcttacctg tcgcagtacc aatcagctgg cactctgcgc ctggctgctc gtccacaatg
     1321 cgtacactct gaccggcagc actcaattcg ttgctggagc tacaacccgc catcagtagc
     1381 gcggcaccga caatccctgc taagtatttt acgtgcatgt tattccccat aatcaatgag
     1441 ctggacgcca ctcgcgtcgt ccgtgtctga atcttatact agaaagatga ccaaaagaaa
     1501 aacccccaga catttctgcc cgggggtttg tttatttctg cgaggtgcag ggcaattaca
     1561 tcatgccgcc catgccaccc atgccgccca taccgccagc agcgcctaag tcagctgcat
     1621 catttttcgg caggtcggta accatgcact cggtggtgat catcaggcca gccacagaag
     1681 ccgcgtactg cagagcagaa cgggttactt tggttgggtc caggataccc atgtcgatca
     1741 tgttgccgta ttcttcggtt gctgcgttgt aaccgtagtt gccgtcgccg cctttaacgg
     1801 tgttagcaac aacagacggt tcttcgccgc agttcaggac gatctgacgc agcggagctt
     1861 ccattgcacg cagtgcaact ttgataccca cgttctggtc ttcgttctga ccacgcaggt
     1921 cagccagttt agacgctacg cggatcagcg caacaccacc accagcaacc acgccttctt
     1981 ctaccgcagc acgggtcgcg tgcagggcgt cttcaacgcg tgcttttttc tctttcattt
     2041 caacttcggt agcagcacct actttgataa ctgcaacgcc gcctgccagt ttcgctacgc
     2101 gttcctgcag tttttcacgg tcgtagtcag aagttgcttc ttcaatctgc tgacggatct
     2161 gagcaacacg gccctggatt gcagcttctt cacccacgcc atcgatgata gtggtggtgt
     2221 ctttgttgat cacaacacgt ttagcctgac ccaggtcttc cagggttgct ttttccagct
     2281 ccataccgat ctcttcagag attacggtac cgccagtcag ggttgcgata tcctgcagca
     2341 tagctttacg acgatcgccg aagcccggag ctttaaccgc agcaactttc acgatgccac
     2401 gcatggtgtt aacaaccaga gttgccagcg cttcgccttc aacatcttca gcgatgatca
     2461 gcagcggttt gcctgctttg gcaacagctt ccagaaccgg cagcatttcg cggatgttag
     2521 agattttctt gtcagccagc aggatgaacg ggctttccag ttctactgcg ccagtttccg
     2581 gcttgttgat gaagtaagga gacaggtagc cacggtcgaa ctgcatacct tcaaccacgt
     2641 ccagttcgtc ctgcagaccg gtaccgtctt caacggtgat aacgccttct ttaccgactt
     2701 tgtccatcgc ttcagcgatc agtttaccta cggtttcgtc ggagttagcg gagatggtac
     2761 caacctgagc aatcgcttta gagtcagagc acggtacgga cagcgctttc agttcttcaa
     2821 ctgcagcggt aacggctttg tcgataccac gtttcaggtc catcgggttc atgcccgcag
     2881 caacagcttt cagaccctca gtgatgatag cctgagccag tacagttgca gtggtggtac
     2941 cgtcgcctgc agcgtcgttc gctttagagg caacttcttt caccatctgc gcacccatat
     3001 tttcgaactt gtcttccagt tcgatttcac gagcaacgga aacaccatct ttggtgatgg
     3061 tcggtgcacc gaaagattta tccagaacta cgttacggcc tttcggaccg agggtaactt
     3121 tcactgcatc tgccagtacg tttacgccgc gcagcatttt cacacgagcg tcgttaccga
     3181 attttacgtc tttagctgcc attatcttta ttccttaaat tcgtatgttc agtgtcgtgc
     3241 gaggattacg cttcaacaat tgccagaatg tcgctttcgg acatgatcaa cacttcttca
     3301 ttgtcgatct tctcagattt cacaccgtag ccatcgttga aaataacgat gtcgccaact
     3361 ttcacatcca gcggcttcac ttcgccattt tcaaggatac ggccattgcc gacagccagc
     3421 acttcgccgc gggtggattt agccgctgca gagccggtca gaacgatgcc gccagcagat
     3481 ttagtttcaa cttctttacg cttgacgatc acgcgatcat gcaatggacg aatattcatt
     3541 gataactctc ctttgagaaa gtccgtatct gttatgggtg acgccggagc ttacgtggtt
     3601 tcccggctgg tgaccagaga aatggggatg aggattcgcc ccttcaaggg ggaaaaagaa
     3661 aaaaaattct gatcagcaca aaatcgggtg aaaaccctga ttcacctcac atttcatcgc
     3721 aatttcttca tcgccgtcat aagcgaatct gattgtgcta ccatcgaaaa tctacgcagt
     3781 tgcccaaaat ttgggcgcaa tcggatatca acccatgagt ggactcaaac aagaactggg
     3841 gctggcccag ggcattggac tgctatcgac gtcattatta ggcactggcg tgtttgccgt
     3901 tcctgcgtta gctgcgctgg tagcgggcaa taacagcctg tgggcgtggc ccgttttgat
     3961 tatcttagtg ttcccgattg cgattgtgtt tgcgattctg ggtcgccact atcccagcgc
     4021 aggcggtgtc gcgcacttcg tcggtatggc gtttggttcg cgacttgagc gagtcaccgg
     4081 ctggttgttt ttatcggtca ttcccgtggg tttgcctgcc gcgctacaaa ttgccgccgg
     4141 gttcggccag gcaatgtttg gctggcatag ctggcaactg ttgttggcag aactcggtac
     4201 gctggcgctg gtgtggtata tcggtactcg cggtgccagt tccagtgcta atctacaaac
     4261 cgttattgcc ggacttatcg tcgcgctgat tgtcgctatc tggtgggcgg gcgatatcaa
     4321 acctgcgaat atccccttcc cggcacctgg taatatcgaa cttaccgggt tatttgctgc
     4381 gttatcagtg atgttctggt gttttgtcgg tctggaagca tttgcccatc ttgcctcgga
     4441 atttaaaaat ccagagcgtg attttcctcg cgcattgatg attggtctgc tgctggcagg
     4501 attagtctac tggggctgta cggtagtcgt cttacacttc gacgcctatg gtgaaaaaat
     4561 ggcggcggca gcatcgcttc caaaaattgt agtgcagttg ttcggtgtag gagcgttatg
     4621 gattgcctgc gtgattggct atctggcctg ctttgccagt ctcaacattt atatacagag
     4681 cttcgcccgc ctggtctggt cgcaggcgca acataatcct gaccactacc tggcacgcct
     4741 ctcttctcgc catatcccga ataatgccct caatgcggtg ctcggctgct gtgtggtgag
     4801 cacgttggtg attcatgctt tagagatcaa tctggacgct cttattattt atgccaatgg
     4861 catctttatt atgatttatc tgttatgcat gctggcaggc tgtaaattat tgcaaggacg
     4921 ttatcgacta ctggcggtgg ttggcgggct gttatgcgtt ctgttactgg caatggtcgg
     4981 ctggaaaagt ctctatgcgc tgatcatgct ggcggggtta tggctgttgc tgccaaaacg
     5041 aaaaacgccg gaaaatggca taaccacata atccggcgtt tcgacattaa tcctggcgat
     5101 cgtctttatg atcaaggcgg tcgcgctcat catcctttcg ctggtactca ccatcaaaag
     5161 tattaccgcc accggtcccg gcgctaaaac cgccgcctgg catacgagaa aagcgcaaat
     5221 gcggcatcaa cttcactgtc agatgctttt gcaccggcgg taataaaagc agaagaccga
     5281 ggaagtcggt gaaaaagccc ggtaataaaa gcagcaaacc ggcaatgatc agcgaaacac
     5341 ttttaatcat ctccgccgct gggttttcac ccgccgccat tttttgctgc atcagcacga
     5401 aattcttaaa gccctggtta cgtaccagtg acatcccgat aactgacgtg aatataacca
     5461 gcacgagggt cagcaatacc cccaatacat gggcaacctg aataaagatt gaaatctcaa
     5521 tatagacata aaggaaaatg gcaataaaag gtaaccagcg caaaggtttc tcctgtaata
     5581 gcagccggta aaacccggct acctgaatgg gttgcgaatc gcgttcagct tatattgtgg
     5641 tcattagcaa aatttcaaga tgtttgcgca actatttttg gtagtaatcc caaagcggtg
     5701 atctatttca caaattaata attaaggggt aaaaaccgac acttaaagtg atccagatta
     5761 cggtagaaat cctcaagcag catatgatct cgggtattcg gtcgatgcag gggataatcg
     5821 tcggtcgaaa aacattcgaa accacatata ttctgtgtgt ttaaagcaaa tcattggcag
     5881 cttgaaaaag aaggttcaca tgtcaaacaa cattcgtatc gaagaagatc tgttgggtac
     5941 cagggaagtt ccagctgatg cctactatgg tgttcacact ctgagagcga ttgaaaactt
     6001 ctatatcagc aacaacaaaa tcagtgatat tcctgaattt gttcgcggta tggtaatggt
     6061 taaaaaagcc gcagctatgg caaacaaaga gctgcaaacc attcctaaaa gtgtagcgaa
     6121 tgccatcatt gccgcatgtg atgaagtcct gaacaacgga aaatgcatgg atcagttccc
     6181 ggtagacgtc taccagggcg gcgcaggtac ttccgtaaac atgaacacca acgaagtgct
     6241 ggccaatatc ggtctggaac tgatgggtca ccagaaaggt gaatatcagt acctgaaccc
     6301 gaacgaccat gttaacaaat gtcagtccac taacgacgcc tacccgaccg gtttccgtat
     6361 cgcagtttac tcttccctga ttaagctggt agatgcgatt aaccaactgc gtgaaggctt
     6421 tgaacgtaaa gctgtcgaat tccaggacat cctgaaaatg ggtcgtaccc agctgcagga
     6481 cgcagtaccg atgaccctcg gtcaggaatt ccgcgctttc agcatcctgc tgaaagaaga
     6541 agtgaaaaat atccaacgta ccgctgaact gctgctggaa gttaaccttg gcgcaacagc
     6601 aatcggtact ggtctgaaca cgccgaaaga gtactctccg ctggcagtga aaaaactggc
     6661 tgaagtcact ggcttcccat gcgtaccggc tgaagacctg atcgaagcga cctctgactg
     6721 cggcgcttat gttatggttc acggcgcgct gaaacgcctg gctgtgaaga tgtccaaaat
     6781 ctgtaacgac ctgcgcttgc tctcttctgg tccacgtgcc ggcctgaacg agatcaacct
     6841 gccggaactg caggcgggct cttccatcat gccagctaaa gtaaacccgg ttgttccgga
     6901 agtggttaac caggtatgct tcaaagtcat cggtaacgac accactgtta ccatggcagc
     6961 agaagcaggt cagctacagt tgaacgttat ggagccggtc attggccagg ccatgttcga
     7021 atccgttcac attctgacca acgcttgcta caacctgctg gaaaaatgca ttaacggcat
     7081 cactgctaac aaagaagtgt gcgaaggtta cgtttacaac tctatcggta tcgttactta
     7141 cctgaacccg ttcatcggtc accacaacgg tgacatcgtg ggtaaaatct gtgccgaaac
     7201 cggtaagagt gtacgtgaag tcgttctgga acgcggtctg ttgactgaag cggaacttga
     7261 cgatattttc tccgtacaga atctgatgca cccggcttac aaagcaaaac gctatactga
     7321 tgaaagcgaa cagtaatcgt acagggtagt acaaataaag aaggcacgtc agatgacgtg
     7381 ccttttttct tgtgagcagt aacttaaaaa taacaaccta atatcaactt gttaaaaaac
     7441 aaggaaggct aatatgctag ttgtagaact catcatagtt ttgctggcga tcttcttggg
     7501 cgccagattg gggggaatag gtattggttt tgcaggcgga ttgggggtgc tggttcttgc
     7561 cgctattggc gttaaacccg gtaacatccc gttcgatgtc atttccatta tcatggcggt
     7621 tatcgccgct atttctgcca tgcaggttgc tggcggtctg gactatctgg ttcatcagac
     7681 agaaaagctg ctgcgccgta acccgaaata catcacgatc ctcgcaccga tcgtgaccta
     7741 tttcctgact atctttgctg gtactggcaa catatctctg gcgacactgc cagttatcgc
     7801 tgaagttgcg aaggaacaag gcgtcaaacc ttgccgtccg ctgtctactg cagtggtatc
     7861 cgcgcagatt gcgatcaccg catcgccaat ctcagcggca gtggtttaca tgtcttccgt
     7921 gatggaaggt catggcatca gctacctcca tctgctctcc gtggtcatcc cgtccaccct
     7981 gctggcggtt ctggtgatgt ccttcctggt cactatgctg ttcaactcca aactctctga
     8041 cgatccgatt tatcgtaagc gtctggaaga gggcctggtt gaactgcgcg gtgaaaagca
     8101 gattgaaatc aaatccggtg caaaaacgtc cgtctggctg ttcctgctgg gcgtagttgg
     8161 cgtggttatc tatgcaatca tcaacagccc aagcatgggt ctggttgaaa aaccgctgat
     8221 gaacaccacc aacgcaatcc tgatcatcat gctcagcgtt gcaactctga ccaccgttat
     8281 ctgtaaagtc gataccgaca acatcctcaa ctccagcacc ttcaaagcag gtatgagcgc
     8341 ctgtatttgt atcctgggtg ttgcgtggct gggcgatact ttcgtttcca acaacatcga
     8401 ctggatcaaa gataccgctg gtgaagtgat tcagggtcat ccgtggctgc tggccgtcat
     8461 cttcttcttt gcttctgctc tgctgtactc tcaggctgca accgcaaaag cactgatgcc
     8521 gatggctctg gcactgaacg tttctccgct gaccgctgtt gcttctttcg ctgcggtgtc
     8581 tggtctgttc attctgccaa cctacccgac gctggttgct gcggtacaga tggatgacac
     8641 gggtactacc cgtatcggta aattcgtctt caaccatccg ttcttcatcc cgggtactct
     8701 gggtgttgcc ctggccgttt gcttcggctt cttgctgggt agcttcatgc tgtaatgacc
     8761 aatcgcgggg cgttcacgcc ccgctttctt tcccgccgac taacatcctt cccccgtccg
     8821 ttgtatagtg acctctctct tgcggttcca tctgttcttg cgaggtgttt atgcttgatg
     8881 aaaaaagttc gaataccgcg tctgtcgtgg tgctatgtac cgcaccggat gaagcgacag
     8941 cccaggattt agccgccaaa gtgctggcgg aaaaactggc ggcctgcgcg accttgatcc
     9001 ccggcgctac ctctctctat tactgggaag gtaagctgga gcaagaatac gaagtgcaga
     9061 tgattttaaa aactaccgta tctcaccagc aggcactgct ggaatgcctg aagtctcatc
     9121 atccatatca aaccccggaa cttctggttt tacctgttac acacggagac acagattacc
     9181 tctcatggct caacgcatct ttacgctgat cctgctactt tgcagcactt ccgtttttgc
     9241 cggattattc gacgcgccgg gacgttcaca atttgtcccc gcggatcaag cctttacttt
     9301 tgattttcag caaaaccaac atgaccttaa tctgacctgg cagatcaaag acggttacta
     9361 cctctaccgt aaacagatcc gcattacgcc ggaacacgcg aaaattgccg acgtgcagct
     9421 gccgcaaggc gtctggcatg aagatgagtt ttacggcaaa agcgagattt accgcgatcg
     9481 gctgacgctt cccgtaacca tcaaccaggc gagtgcggga gcgacgttaa ctgtcaccta
     9541 ccagggctgc gctgatgccg gtttctgtta tccgccagaa accaaaaccg ttccgttaag
     9601 cgaagtggtc gccaacaacg cagcgtcaca gcctgtgtct gtttcgcagc aagagcagca
     9661 caccgcgcaa ttgccctttt ccgcgctctg ggcgttgttg atcggtattg gtatcgcctt
     9721 tacgccatgc gtgctgccaa tgtacccact gatttctggc atcgtgctgg gtggtaaaca
     9781 gcggctctcc actgccagag cattgttgct gacctttatt tatgtgcagg ggatggcact
     9841 gacttacacg gcgctgggtc tggtagttgc cgccgcaggg ttacagttcc aggcggcgct
     9901 acagcaccca tacgtgctca ttggcctcgc catcgtcttt accttgctgg cgatgtcaat
     9961 gtttggcttg tttactctac aactcccctc ttcgctgcaa acacgtctca cgttgatgag
    10021 caatcgccaa cagggcggct cacctggcgg tgtgtttgtt atgggggcga ttgccggact
    10081 gatctgttca ccatgcacca ccgcaccgct tagcgcgatt ctgctgtata tcgcccaaag
    10141 cgggaacatg tggctgggcg gcggcacgct ttatctctat gcgttgggca tgggcctgcc
    10201 gctgatgcta attaccgtct ttggtaaccg cttgctgccg aaaagcggcc cgtggatgga
    10261 acaagtcaaa accgcgtttg gttttgtgat cctcgcactg ccggtcttcc tgctggagcg
    10321 agtgattggt gatgtatggg gattacgctt gtggtcggcg ctgggtgtcg cattctttgg
    10381 ctgggccttt atcaccagcc tacaggctaa acgcggctgg atgcgcgtgg tgcaaataat
    10441 cctgctggca gcggcattgg ttagcgtgcg cccacttcag gattgggcat ttggtgcgac
    10501 gcataccgcg caaactcaga cgcatctcaa ctttacacaa atcaaaacgg tagatgagtt
    10561 aaatcaggcg ctcgttgaag ccaaaggcaa accggtgatg ttagatcttt atgccgactg
    10621 gtgcgtcgcc tgtaaagagt ttgagaaata caccttcagc gacccgcagg tgcaaaaagc
    10681 gttagcagac acggtcttac ttcaggccaa cgtcacggcc aacgacgcac aagatgtggc
    10741 gctgttaaag catcttaatg tccttggcct accgacaatt ctcttttttg acggacaagg
    10801 ccaggagcat ccacaagcac gcgtcacggg ctttatggat gctgaaacct tcagcgcaca
    10861 tttgcgcgat cgccaaccgt gaacgacact tgcagtggga aagacggagg aaaatagcgt
    10921 gcaacgtgaa gatgtactgg gagaagccct gaaattatta gaattacaag ggattgccaa
    10981 caccacgctg gagatggttg ctgaacgtgt ggattatcca ctggacgagc tacgccgctt
    11041 ctggccagac aaagaggcaa tcctctacga tgcgctgcgt tatcttagcc aacagataga
    11101 tgtctggcgt cgtcagctga tgctggacga aacgcaaacc gccgaacaaa agttgctggc
    11161 acgttatcag gcgttatcgg agtgcgttaa aaacaaccgc tatccgggct gtctatttat
    11221 cgctgcctgt acgttttatc ccgatcctgg ccaccctatt catcaactgg ccgatcagca
    11281 aaaaagcgcg gcctacgatt tcacccacga actgttaacc acgctggaag ttgacgatcc
    11341 ggcgatggta gcaaagcaga tggaactggt gctggaaggc tgtttaagcc gaatgctggt
    11401 gaatcgtagc caggcggatg tcgacactgc acatcggctg gcggaagata tcctgcgctt
    11461 cgcccgctgc cgccagggtg gtgcattgac ctgacagaaa cacagaaaag aagcgatttg
    11521 ccgcaatctt aagcagttga atcgctttta ctgaaattag gttgacgaga tgtgcagatt
    11581 acggtttaat gcgccccgtt gcccggatag ctcagtcggt agagcagggg attgaaaatc
    11641 cccgtgtcct tggttcgatt ccgagtccgg gcaccaaatt catatcaacg gacctccacg
    11701 gaggtccgtt tttcgtttca gaacgccaca atttaagcgt tctgcctcca aatcaattct
    11761 accgaactca accagattct ccccacatca acctcattgt gtgggtataa ttgcgggtat
    11821 accccagttc gacagaattt gtacccgctt taacgcattc agaaggctga actatggcac
    11881 tgactgacgc aaaaatccgg gctgcaaagc ccactgacaa ggcttataaa ctcactgacg
    11941 gggccggcat gttcctgctg gttcatccta atggttcccg ttactggcgt ctccgttatc
    12001 gtattctggg taaggagaaa accctggcac ttggtgtgta tccggaagtt tctctctccg
    12061 aagctcgtac aaaacgggat gaggcccgaa aactgatttc agaggggatt gacccttgcg
    12121 aacagaaaag agttaaaaaa gtagtccctg atttacagct ctcttttgaa catattgcac
    12181 gacgctggca tgccagtaat aaacaatggg cacaatcaca cagcgataaa gtactcaaaa
    12241 gcctcgagac acacgttttc ccctttatcg gcaaccggga tatcacaaca ctcaataccc
    12301 cggacctgct tattcctgtt cgtgctgcag aagcaaaaca aatttatgaa atcgccagtc
    12361 gtctgcagca aagaatatct gctgtaatgc gttatgccgt acagtctggc atcatcagat
    12421 ataatcctgc tctggatatg gctggcgcat tgacctctgt aaaacgacag catcgccccg
    12481 ctcttgatct ttcacgtctg cctgaacttc tgtcgcgtat taacagttat aaaggtcagc
    12541 ctgtcacccg gcttgctgtt atgctgaatt tactggtttt tattcgctcc agtgaactca
    12601 gatatgcccg ctgggctgaa attgatattg acaattcaat gtggactatt ccagccgaac
    12661 gcaaacctct gccaggggta aaattctcac accggggctc caagatgcga acaccacatc
    12721 ttgtgccact cagcaaacag gctgtagcca tactgacaga acttcagaca tgggctggtg
    12781 aaaatggtct gatctttacg ggagcacatg acccgcgtaa accaatcagt gaaaataccg
    12841 taaataaggc cctcagagtg atggggtatg acacaaccaa ggaagtctgt ggccatggat
    12901 tccgggcgat ggcgtgcagt gcattgattg aatccggctt atggtcaaga gatgcggttg
    12961 aacgtcagat gagtcatcag gagcgcaacg gagtacgtgc cgcgtatatt cataaagcag
    13021 aacatctgga agaacgtcgg cttatgctgc aatggtgggc agattttctg gatgcaaaca
    13081 gggaggagtg tatcagtccg tttgaatatg caaaagttaa caatccactg aaaaggtgac
    13141 tgaacaccat ttacacacat gctcttcctc ttaaggggaa gagcacccgc ctcaatcaat
    13201 ttctttttta tgccggcgga accatcagcc aggctgaggt tctcattcaa taactcaacg
    13261 tcttaccaca gaacaggatt cactgtatta tcatgggcaa cgttaatccc ggacaataaa
    13321 aggacaccga tgccaggcat cacaattttt atagcctgca gattatggtc catccttttt
    13381 tacaggttca gaatatgact ggcaaattac gctttgaagt gaacgataac cagggctgtt
    13441 tcatatttcc ggaaacctgg tttggctcgt tgctggatga gtttgaagaa ctgattgatg
    13501 catacgatgc ggatgaaatc agcgaaacaa gttacatcaa caaactcagg cgccttgccc
    13561 gacaggagaa tgactttatt gatgtacacg ctcacttggc atacgtattt ctggagcaga
    13621 atgccccgcg taaggcgctg aatgctgccc ttaaagggct ggcagttggc aatcgtctta
    13681 tccctgaagg tttcagtggc agaatcatct ggatacatcc tgacaaccga ccttttcttc
    13741 gtgccctcta tgcggcaatc ctggcaaatg cacatttaca gcgtcatcag gatgccatca
    13801 tgctcattga aaaaatactg gattacaatc ccgaagataa tcatggtgca cgctggctac
    13861 tgggaccgga actgttacgc actggcgctc atgaacaggc acgccatata ctgcaggaac
    13921 atgcagatga attttcacct tactggtatg aactcggcct tctgcatttt cttaatgggg
    13981 aactggtaaa agctgcaact gcatttcgtc gcggctttgc ggcaaatact tacatcgcag
    14041 aaatactgtg cggaaatctg catccatttc cacttgctgt atggcataac ttttcaggcg
    14101 gtccggatac tgccgaagat tattatgcca cataccaccc gttatggggg caatatccgg
    14161 aagcattgct gtttgtaaac tggctctata atcattcctc tgtgctgcat gaacgggcag
    14221 aaatcattaa atgcgccgaa atgctgatgc aggaagatga tttcgaaata tgcgaaagta
    14281 ttttaagaca gcaggagaag ttgcgtgaaa gaattgatga gacgctttct gagaaaattg
    14341 tacagaaatg cagaaatatg aatggtgaat atgtctggcc ctggatattg ccgttttcag
    14401 cggcaggcat gaaacatact ggcatacagt atcagtagat attgcattag tgtatcctgc
    14461 acacaagtaa taatttatcc accaataata acactgttaa tgtccccttc ccctggttgt
    14521 cagccagggg ttatcttctg aatatttctt ttgaaaa
//
LOCUS       DBANUU010000110        14534 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000110 DBANUU010000000
VERSION     DBANUU010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.110"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            129..485
                     /locus_tag="ACIE68_003438"
     CDS             129..485
                     /locus_tag="ACIE68_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFX5909700.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(536..1432)
                     /gene="yhaJ"
                     /locus_tag="ACIE68_003439"
     CDS             complement(536..1432)
                     /gene="yhaJ"
                     /locus_tag="ACIE68_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFX5909701.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1537..2238
                     /locus_tag="ACIE68_003440"
     CDS             1537..2238
                     /locus_tag="ACIE68_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFX5909702.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2261..2425
                     /gene="yhaL"
                     /locus_tag="ACIE68_003441"
     CDS             2261..2425
                     /gene="yhaL"
                     /locus_tag="ACIE68_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFX5909703.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2637..3947)
                     /gene="cyuA"
                     /locus_tag="ACIE68_003442"
     CDS             complement(2637..3947)
                     /gene="cyuA"
                     /locus_tag="ACIE68_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFX5909704.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3975..5306)
                     /locus_tag="ACIE68_003443"
     CDS             complement(3975..5306)
                     /locus_tag="ACIE68_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFX5909705.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5581..6945)
                     /gene="tdcG"
                     /locus_tag="ACIE68_003444"
     CDS             complement(5581..6945)
                     /gene="tdcG"
                     /locus_tag="ACIE68_003444"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFX5909706.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(7017..7406)
                     /locus_tag="ACIE68_003445"
     CDS             complement(7017..7406)
                     /locus_tag="ACIE68_003445"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFX5909707.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7420..9714)
                     /gene="tdcE"
                     /locus_tag="ACIE68_003446"
     CDS             complement(7420..9714)
                     /gene="tdcE"
                     /locus_tag="ACIE68_003446"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFX5909708.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9748..10956)
                     /gene="tdcD"
                     /locus_tag="ACIE68_003447"
     CDS             complement(9748..10956)
                     /gene="tdcD"
                     /locus_tag="ACIE68_003447"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFX5909709.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10982..12313)
                     /gene="tdcC"
                     /locus_tag="ACIE68_003448"
     CDS             complement(10982..12313)
                     /gene="tdcC"
                     /locus_tag="ACIE68_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFX5909710.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12335..13324)
                     /gene="tdcB"
                     /locus_tag="ACIE68_003449"
     CDS             complement(12335..13324)
                     /gene="tdcB"
                     /locus_tag="ACIE68_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFX5909711.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13423..14361)
                     /gene="tdcA"
                     /locus_tag="ACIE68_003450"
     CDS             complement(13423..14361)
                     /gene="tdcA"
                     /locus_tag="ACIE68_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFX5909712.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3794 a         3891 c         3377 g         3472 t
ORIGIN      
        1 ataaaaaaca accatgtatt tatcttaaat atcaaaacct ttataacatc cgaaaaagaa
       61 agatacgaat agcatttaca caatacccgg aatagacttt cgtcactttt aattaaaggg
      121 atgtttttat gcagtggtat ttagccgtat taaaaaatta tgttggtttc tctggtcgcg
      181 cacgtcgtaa agagtactgg atgtttactc tgattaacgc catcgtcggc gctattatca
      241 atgtcattca attgatttta ggtttagagt ttccattcct ttctctcatt tacctggcag
      301 caacaatcat tccggtcatt gctctgtgtg ttcgtcgtct gcacgatact gaccgttcag
      361 gtgcgtgggc gcttctctat ctggtaccga tcattggctg gttagttctg tttgtatttg
      421 cctgtttaga aggcaattct ggcagcaatc gttacggaaa cgatcctaag tttggttcaa
      481 attaatctta gaattggggc gacatttcgc ccctttttat taataataac aaatattatt
      541 ttccgctaaa aagtttggga atttcccgca gacaccagga tttcgcttcc cccatactgt
      601 cacgacgcca ggccataata atatcgatct cgctggtcga ttccgggctg acgacacgca
      661 accgcccttc cgcaatatct ttttcgacca tcggatacgg catcgtcgcc acgccaagcc
      721 ccgccagtaa tgcctgacgt ttatcttcaa tcgtgctcac cgttaagcgc ggctgtttgt
      781 ccagcagctg tacggtcaac accgggcgct cacgagcggt atccgctacc gcaattccac
      841 gatatttcac gcgcgtcact tcagataacg gttccggctc ctgatgaatc gggtgatcag
      901 gcgcggcaac gtagacgttc attaaggtat agagcttgcg cgagttgatc tccgacgagg
      961 aacgaaaatg catatccggc gcgataacga tatccgcccg cccctgctcc agtcgttccc
     1021 acgcccccgc cagcacttcg gtgatgattg ccagttgggt attggctttt gccgccagtt
     1081 tgtcgattaa cgggaaaaag gcaggtgtcg gtaccagcgc ctcggtcaca atggtgagat
     1141 gcgtttccca accgcgcgcg agggcttccg catcggtagt cagtttatct gcggcttcca
     1201 gcaaaacgcg ccctcgctcc agcaacatcc tcccgacatt ggtgaatttg gtgcgatggc
     1261 ccgagcggtc aaacagcacc acatccagct cttcttccag tttttgcatg gtgtagctaa
     1321 gtgcggaagg cacgcgtccc agctcatccg ccgccgccgc aaaactgccc cggcgatcga
     1381 tcgcatccat aacccgtagt gcttccagcg ttaatgccct ttctttggcc atttcgttct
     1441 cattcaggaa atttgaacat accgggcaga atatctggct aacaatgtag cgtccagccc
     1501 cttaccataa aaggaagtaa agagaggtca agaattatga ttactacccg aactgccaga
     1561 cagtgtggac aagcagacta cggatggttg caggcccggt atactttttc ctttggacac
     1621 tacttcgacc cgaaattgtt aggctatgcc tccctgcgtg tgcttaacca ggaagtgctg
     1681 gccccaggtg ccgcctttca gccgcgaacc tatcccaaag tcgatatttt aaatgtgatt
     1741 ctggatggag aagcagagta tcgcgacagc gaaggcaatc atgttcaggc cagcgccggt
     1801 gaggcgttgc tgctctctac ccagccgggt gtcagctaca gcgaacacaa tctcagcaaa
     1861 gacaaaccgt taacacgaat gcagctttgg ctggacgcct gcccgcagcg agagaatccg
     1921 ctgattcaaa aactggcgct taatatgggc aagcagcaat taatcgcctc gccagagggt
     1981 acgatgggaa gcctgcaatt acgccagcaa gtgtggctgc accatatcgt gctcgacaaa
     2041 ggcgaaagtg cgaatttcca gttgcatggg ccacgcgcgt atttgcaatc gattcacggg
     2101 aaatttcatg cgcttacgca tcatgaagag aaagcggcgc tgacctgcgg tgatggggcg
     2161 tttattcgtg acgaggctaa cattacgctg gttgccgatt ccccactgcg cgctttgctg
     2221 atagatttgc ctgtctagtt gtttttacag ggagatgatg atgagtaaaa aatcggccaa
     2281 aaagcgccag ccagtgaagc ccgtggtggc gaaagaacct gctcgcaccg ccaaaaattt
     2341 tggctatgaa gagatgttga gcgagctgga agccatcgtc gcggatgctg aaacgcgttt
     2401 agccgaggat gaagctaccg cgtaaagaca ggttgccgga tgcggcgaaa acgccttatc
     2461 cggcctacaa aatccaacaa cattaacgaa ttaaacaacc cgtaggccgg ataagatgcg
     2521 ccagcatcgc atccggcatc aggtacaaaa cacaacaaca tcaacaagtt gagcaccccg
     2581 taggccggat aaggtgcgcc agcatcgcat ccggcacaat actcaaaacc tggcgtttat
     2641 ctggccttgc tcgccataat ctcgataatc tgccgatccg tttgctgcat cgaatggctt
     2701 gctaacgcac acaggttggc aatcgactgc tcaacatcat gcgccacgat cccttcattg
     2761 ccggtcacgg cggtatcatc cagcgccatt aacaccgctt tccacgcagc cgaagcactg
     2821 gtcgaaacct tcatcgcgca gctgttcgac gcaccatcgc aaatcatgcc gctgacatcg
     2881 ccgatcatac tgctgatcgc catcgagatg gtttcataac gcccatccac cagccatgcc
     2941 atcccggcgg cggcccccat tgctgcggtc gttgcggcac acagcgcaga caaacgcggt
     3001 aactggttat ggatgtaaat tgcgctcaaa tgagaaagca tcagcgcacg cgccagccgt
     3061 tcatcatccg ctccgaagtg ttctgctacc accaccacag gcattgttgc ggtgatcccc
     3121 tggttacccg agccggagtt actcatagcc ggaagcgtag cgccgcccat acgcgcatcg
     3181 gatgccgcgc tggtacgaat cacaatggat gaagagagat ctttcgccag caaaccgcgc
     3241 tcgcactgtt tttccagcgt cgcgccaata tgcagccccc acttaccgct caaaccttcc
     3301 tgcgataacg cacaatttag cttcgcggaa tcgagaataa agcggatcgc cgcaaacggg
     3361 acttcattga cgaacttcag gatctcagcc agcgtcgttc tggaaagcac cgtcagcgga
     3421 gactcttgct cgccctctgc cacacacgcc tgctgggtaa acaccacacc attgtgcgtc
     3481 tcaatatgca caatgttggt atgcccgccg acgatggtga cacacgccca cttctcaccg
     3541 ttccagactt tggcgcgtga gaagaggatt tcattgcaag gttcctggat cttaacggag
     3601 actttccccg ccgccagcag tgctttggca tcggcaattg cctgcgcagt tgcgtctttc
     3661 agcacttcca gcccggcgtt ggcatttcca cctaacgccc ccagcgccgc cgcaatcggc
     3721 agccccacca ttcccgtgcc gggaacggtg acgcccagac cgttcttcat cagatttggc
     3781 gaaacccagg cttctacacg ttcaaccgga ccttccagtt ctgccgcagc aaccgccgcc
     3841 gccagcgcca gtgaaatcgg ttcagtacat cccagcgccg gttttacttc ctcctgaacg
     3901 gcgaggatgt aacgctgcca taacggattt aaagtcgaat caaacatatt aaaaacctta
     3961 aaatttcagg taaatcaaga aaatgccagg aacggagaaa cacaaagcaa caaaccagtg
     4021 acgataatca ggtacagaga catccctttg tatttgtgca atgccggtac tttgtaaacc
     4081 agccacgccg ggatcaggca ccctaccatg ccgaaaatcg ggctacagat agaggtgaag
     4141 ctcaacaccg gtgcgttcag tacgatggcg ctccaggcca gcaaaatggc gaaaatcatg
     4201 atgccgcgct gaacaagatt ttcgttaatc ttctcggcag gcatcttgcg acgcaggatg
     4261 ttcattacga tcccttgcgt tgcttcgcga aagcctaaat agacgccaaa gaatgcagtc
     4321 atgacagcaa agatattgag aatgacgctg accactttca cccacgctgc gccgtcaccg
     4381 ctaataaact gtgcggcaat cgccagcgcg gaaatattct gctcataggc ttttaccgct
     4441 tcgtcatgtc ccatcgccag cgtgaacgac acggcgtaga aaaagacggt gacaaacaaa
     4501 atgccaaacg cgatattcat tgcccgcaat gctttatgcc gcgccacttc aattgatttt
     4561 tcccgcgagc gataagagat caccatcggg cttaacgtct ggataaacag aatcgacgtc
     4621 agggtaaacg gcagcgtaat aatggcgttt ttcaccagca gccccagcgg cggtagcgaa
     4681 ccgacgttat acagatgcca cattcccacc atcgacacgc ccagcgccgc gaccaccagc
     4741 agcttggtca gcaccatgcc ggtcgaaatt ttgaataaca atttctcgcc gcgtgaagag
     4801 atcgccacca gaatgcaaat cagtaccaga ccataaaagg gactgtctga cagcaaccct
     4861 tccgtcacgc cgaaggtatg cagataggaa gcactatcgt tggtgatggc ggtggaatag
     4921 acgaacatcc aaatcaccag cattacgaaa tagagcgcac ctaacaggat gccccagttt
     4981 ttacctaaat aaccgctaat gacactcggg taatctttac attctggtga ttctgccagc
     5041 gtattaataa acaaccgctg aaacagatac attgccgggt aaccaatcac cgatgagagc
     5101 aaaaataccc acaatcccat caaaccgacc tgcaccggga gaaaaacaat ccccgcgcca
     5161 atcgccatcc cgatactcat aatcacccag ccggtgtcag tactgtcgaa tttgatcgct
     5221 tctcgccact cgctctcact cattccagca cgacccgccg gggtcgaagc gtctgcaatg
     5281 acgcctttat tcgatgcaat ttccataatt tctcgctcaa tattttgtag ggcttattat
     5341 ttttttccga gccgcatcaa ggcgatatgc ggtctacgtt tttgcaggcg agttattaga
     5401 ataaagaaat gatacgcgcg aggtcagaga aaatcttcac aatcaagact ttgaaataac
     5461 ggagatggat aagaattttc tactaaatta atcgcaagag aatattcatt ctctatttgc
     5521 ggcagatcac aaaaaaaagg tgcacatttg tgcacccaag gatgaaagct gacagcaatg
     5581 tcagccgcag accactttaa tggccagtcc tccgcgtgat gtttcgcggt atttatcgtt
     5641 catatctttg ccggtttcat acatcgtctc gatcacttta tcgagtgaaa cacgcggtgc
     5701 cgaggtgcgg cgcatcgcca tccgcgcggc gtttactgct ttcacggcat taatggcatt
     5761 acgttcaatg cacgggattt gtacctgtcc ggcaaccgga tcgcaggtca gcccaaggtt
     5821 atgctccatc gcgatttccg ccgcattgca tacctgcgcc ggactgccgc ccagtagttc
     5881 agttaaccct gccgccgcca ttgaacaggc cacgccaatc tccccctgac agccgacctc
     5941 cgcgccagag atggaggcgt tcattttata cagcgcgcca atagcccccg ctgccagaaa
     6001 atagcgggca attgaccgct cgtttaccgg acgacggaac ttatcgtaat aagccagcac
     6061 tgccggaata atgccgcacg caccgttagt cggtgccgtt accacgcgcc cgccagctgc
     6121 gttttcttca ctaaccgcca gcgcgtacat gttgatccag tcgatgacat tcatcggatc
     6181 gttagagatg ttatcgctag aaaccagctg acgacgcagc gctacggcac gacgcggcac
     6241 attgagcgga ccaggcagca cgccttcagt gttcatcccg cgttcaatac cgtcatgcat
     6301 cacttgccag atacgggcaa accctgcgtc aatttccgct ttactgcgca gcgctagctc
     6361 gttgtgcatc atcaggccag atatagacag gccgttgtaa tcacacattt tcagcagttc
     6421 acctgctgag tggaaatcgt aaggtacgga cgtttcgaca tcgtgcgaca ggccgaagtg
     6481 ttcttcttcg acaataaacc cgccgccgac agagtaatag gttttactta atagcgcttc
     6541 ctgtcccttc caggcagtga tccgcattcc gttctcatgg cgaggcaaca tttcgggatg
     6601 gaagataatg ttctttgcta caggaaaatc aacaatatgc gcaccagatg ccactggcag
     6661 ccgcccgctg cgcgttacta actctataaa tgcagggatc tcatcaatga caacatcctg
     6721 cggactgttt cctgccaatc ccatgataat ggcgacatcc gtggcatggc ctttgcccgt
     6781 cagtgacaac gacccataca gatcgaccac aatatggctc gtcgcggtta ataagccgct
     6841 actttccagc cgatcaataa aactttttcc ggcattcatt ggccccacgg tatgcgaact
     6901 ggagggacca atcccaattt tgaaaatatc gaatgcacta atcatatcca caccctcgga
     6961 ttgccgttca gtgaagtgga gcggaacgac cttgcgaccg tcccgctcac gaggctttac
     7021 gcactacgta ctgcgatagc ttcaatttcc agcttcacat ctttcggcaa acgcgcaacc
     7081 tgcacacagc tccgggtcgg gtaggtcgcc tgatgctcat cgaagaactg cttatagact
     7141 tcattgatgg tggcaaaatc attcagatcg gtgatgaaca cggtcatctt gatgatatcg
     7201 cccacgctca gcccggcagc aaccacgatc gctttgacgt tttcgaggct taaacgcgcc
     7261 tgatcttgca catcagccgg gatctcaccg gtctgcgggc aaaccggaat ttgcccggag
     7321 gtgaagacca tgctgcctaa atcaacgccc tgaacataag ggccgattgc gcctggggca
     7381 cgttgcgttt cgataatctt tttcatacat cctccggcgt cagagcgcct gggtaaaagt
     7441 acgtgaaata acatcctgtt gctgttcacg ggtcagtgcg ttgaagcgca cggcgtagcc
     7501 agagacacgg attgtcaggt taggatattt ttccgggtgc tcgatggcat ccagcagcat
     7561 ttcccgattc attacgttga cgttgaggtg ttgaccgcct tcgacatccg cttcgtggtg
     7621 gaaataccca tccagcaggc cgacaaggtt ggttttacgt actggatctt ctttgcccag
     7681 cgccgcagga acgattgaga aggtgtacga gatcccatct ttggcgtagg tgaacggcag
     7741 tttcgccacc gacgtcaatg aggccacggc acctttgcgg tcacgaccat gcatcgggtt
     7801 agcgcccggc gcgaacggtg ttccggcgcg acgaccgtcc ggcgtattac cggttttctg
     7861 gccgtacacc acgttagaag tgatagtcag aatcgactgg gtagggacgg cgttgcgata
     7921 ggttggcagc gctttaattt tcttcataaa gcgttcaacc aggtcgcagg caatgctgtc
     7981 tacgcgctcg tcgttgttgc cgtactgcgg atattcaccg tcgatttcaa agtccaccgc
     8041 caggccgttt tcgtcacgga ttggtttcac gcgggcatat ttgatggcag acagggagtc
     8101 cgtcgccacc gacaggcccg cgatgccgca tgccatagtg cgatagacat cacgatcgtg
     8161 cagcgccatc agcgaagctt cgtagctgta cttgtcgtgc atgtagtgaa tgatattcag
     8221 cgcgctgatg tactgcaccg ccagccagtc catgaagtga tcgaggctgt ccatcacttt
     8281 gtcgtaatcc agcacgtcgt ccatcagcgg tgctgttttc ggcccgacct gaatcttcag
     8341 cttctcgtcc accccgccgt taattgcgta gagcagcgtt ttcgccaggt tagcgcgtgc
     8401 accaaagaac tgcatttgct taccaatcac cattgggctg acgcagcagg caatcgcgta
     8461 atcgtcgctg ttgaagtcag tacgcatcag atcgtcattt tcatactgca aggaagaggt
     8521 gacgatcgac acctgcgcgg catatttttt gaaggcaatc ggtaattctt ccgaccaaag
     8581 aatggtcagg ttaggttccg gtgccggccc catagtgtgc agggtgtgca aatagcggaa
     8641 ggagtttttg gtcaccagcg tacgaccgtc cagccccatc ccgccgatca cttccgtcgc
     8701 ccagattggg tcgccggaga acagcgaatc aaattccggt gtacgcagga agcgtaccat
     8761 acggatcttc atgatgaagt gatcgatcag ttcctgtgcc tgctgctcat tgagtacgcc
     8821 agctttaaag tcgcgctcaa tgtagatatc gaggaacgat gccgtgcggc ccagcgacat
     8881 cgcgccgcca ttttgcgatt tcactgccgc cagataagcg aagtagagcc actgcaccgc
     8941 ttcctgcgca ttctgcgccg ggcgagagat atcaaagcca tatttcgccg ccatttcctg
     9001 aatctgcaac agcgcatgac gatgctctgc cagctcctca cgcagacgga tggtggcttc
     9061 cagatcctcg cctttttcca gacgagactg gagatcggca aattgcagtt cgcgttcacg
     9121 taccagataa ctgatgccat acagcgctac gcggcgatag tcaccgataa tgcgcccacg
     9181 gccatagcca tctggtaaac cggtcagcac gccagattta cggcagcgca gcatatccgg
     9241 tgagtaaaca tcaaatacgc cctggttatg ggttttacgc agatcggtaa acagatattc
     9301 aaattcactg tccatttctc ggccataggc gtggaatgaa cttttaatca tattaatgcc
     9361 accgaacggg tgtagcgcac gtttcaacgg cgcatccgtt tgcaggccaa caattttttc
     9421 cagcggctgg ttaatatatc ccgcatcatg agcggtaatt gtggtggcaa tattggtatc
     9481 gaaatcaacc ggcgcgtggg ttgcattttc gatacggatg ccttccatta ctttttccca
     9541 caattccgtg gtggcaggcg tcgcttcggc gaggaaagat tcatcgcctt catacggtgt
     9601 atagttatgt tgaataaaat cgcggacatt aatttcgttt ttccagtccg tacctttaaa
     9661 gccaagccat gcgtcggcgt acagcttatc gctggtatca atatctacct tcatgaaaaa
     9721 taatctctct acaatacttc aactaaatta tgcaaattct gcgggcgcgt taactttgcc
     9781 taaatgaatg gcatccaaag caatcatttt ttcttcgtta gtcggaataa cggcacaaat
     9841 gacacgcgca ttttcactgg aaacaattcg ctctccaaaa gagttagatc gattattcat
     9901 ttctgtatca atcactacgc ctaatacagc caaatgttcc atgaccagac gacgaattaa
     9961 acttgaattc tctcctattc cgccggtgaa tataattcca tccaggcgac gtaatgaagc
    10021 tgcgtgtccg gcaatatgac gggcaattcg gtgaacaaag gttttaattg ccagttgcgc
    10081 gcgttcgtga ccttcatgcc aggctttttc cagaacacgt aaatccgaag aaagaccgga
    10141 aatacctaat aatcccgact ctttattcac tacgcgttcc aggtcaccca gactctggtt
    10201 ggtttggctg gcgacccagg acatcgcacc aaagtcgaca tcgccactgc gggtacccat
    10261 catcaagcct tccagcggcg tcattcccat tgaagtatca acactctgac cgttgcgaac
    10321 cgcgcagatt gacgcgccat tgccaagatg cgccacaacc aggccggagt catcttccgc
    10381 cagattcagc agcgaatgtg cgcgctggga aacatagcgg tgcgacgtgc cgtggaaacc
    10441 ataacggcgt acacctaact cttcataata tttccacggc aggccgtata aataagcttc
    10501 cggagccatc gtctggtgga aactggtatc aaataccgcc acctgagtta cgcccggaaa
    10561 taattgctgc gccgattcaa taccacttaa attggcgtaa ttatgcaggg gtgccagtgg
    10621 agaaacgcga cggatattat caatgacttc atcggtaata atggcggact cggtaaaaat
    10681 actgccgccg tgagcgatgc ggtggccaat taaggccaca ctgtcattta aattccgttt
    10741 ttccagttca aatgcaattg ccttcaatgc accttcgtag ctgtggtgag ccagcggtgc
    10801 tggctctccc ccatttacgg ataagaatgc attttccgag ttaataccgt cggcaatacc
    10861 tgacattaat acttcacagt cgctggcatc gagcacggaa aacttaatcg aagacgaacc
    10921 acagttaata accaaaacaa ccggaaattc attcatctct tttctcatcc tgagttacgg
    10981 attaaaacag tttgtatacg atgttcagga tggtcagcag accaatcacg gtaacaaaca
    11041 cgttatccag acgaccacgg tatttcgcca gagacggcgc tttacggatg gcatacatcg
    11101 gcaacaggca cagcagggat gcgataatcg gtgcgcccat ggcttcaatc aggtcaagga
    11161 tgttcgggtt ggcgtaggca acaacccagg tggagcccat gatgaagatc atgctgatag
    11221 tgttcagttt acccagcgac actttagttt tgtcgccttt ataaccaaac ttcaggacca
    11281 ggccattcag accttccagc gttcccagat agtgaccgaa gaaagatttg aagatagcca
    11341 cgagtgcgat gatggaagcc gcatattcca gtgtaatcgc gaacgttgtt ttggtaccgg
    11401 tcatggacgc aaagtggtta gccagataag aaagcactgg aatattctgc gctttggctt
    11461 ccgccatgtt ggccggagac agagtaaaca ggcagctaaa ggcaaagaac atcaccactg
    11521 caaccatcag catgctggca cgagaaatga tttgggaaca tttacgttcg gtgaagtcgc
    11581 gaccgaagtc tttctcatac tcttcacgct tagaaaccac gaaggaagag acgattggcg
    11641 agaagttaaa ggagaaaacc atgatggaaa tccccagcca gacagtgatc aggataccgt
    11701 catgaccggt taacgacagc gaaccgaggt caacctggtc gataactgca gagttccagt
    11761 aagggatcag cgacaaagaa atcagcacca ggctggcgat aaacggccat accaggtagc
    11821 tcatcacttt aaccatcaga tccttaccaa accagatgac gaaagccatc agcagcaaca
    11881 ggaacagcgc cacaaagccg cgattcagcg gtgcaaagcc gagctggttt tcccagaacg
    11941 tcataaaggt attggtaata gtaacgccat aaatccacag cagtgggcaa atcgcgaaga
    12001 agtacaggaa cgtgataacc acgccgccag ttttaccaaa atgctcttcc accgtttccg
    12061 taatgttgcc ggaagggtta gagccagaaa gacacagacg cgccagcgcc cggtggcaat
    12121 aaaacgcgat ggggtatgcc aataccaaca tcagaagaat cgggatcagt ccgccaaaac
    12181 ctgcgcggat agggaagaac agcaccccgg cgccgattgc cgtaccaaac aagcctaacg
    12241 tccatgtggt atctgattta cgccaggacg attgttttgt ctggctggat acaatgctat
    12301 ctgaagtact catatcctat cctcaacgaa ttaattaagc gtcaacgaaa ccggtgattt
    12361 gagagacgcg agaaagatcg atattgccgc cggaaataat actgacggtt tttctgtttt
    12421 gaatatattg gtctaattta ccgcttaata atgcagcaca tgccagagcg cctgcgcctt
    12481 cggtgacgac tttattgcgc tgaattaagg caatcatact gtttctgatt tcgtcttcgc
    12541 tgaccagcac gatgtcatcg actaattcac gaacgatttc gtaagttaaa ttacccgggc
    12601 gggagacatc acaaccatcc gccagggtgc cggtagttcg gtgcgtggtt atttctccgg
    12661 agtggaaaga agccgccatg ccgtgaacgt tttcagactg tacgccaata acacgaatgg
    12721 tcgggttaat agatttaatt gccaccgcaa taccagcaat taaaccgcca ccaccaattg
    12781 gcacaatcac gttatcgaca tcatagagat cttccataat ttccagacca atcgttccct
    12841 ggccagcaat cactttcgga tcatcgtaag gtgggataaa aatacggcct tccatttcga
    12901 caatttcgct cactttagcg atagtgtcgt tgaagttatc accatgcaga acgacttctg
    12961 cggagtagtc gcacgttgcc gctactttgg attttggcgc accttttggc atcaccactt
    13021 taccgtcgat acccagcatc gcgcaggaca gggaaacccc ttgcgcatgg ttgcccgcag
    13081 aacaggccac cacgcctttg cgtttttccg catcggtcag tgaacttaat ttattaaatg
    13141 cgccacgaat tttaaatgaa cccgtacgct gcatattttc aaacttcagg aatatttcac
    13201 ctttgcaacg ttcactaaaa tagttggagc gaggcatgcc tgttttataa attcgcccag
    13261 ccagtcgttg tttcgcttca ataatgtcat caatagcaac cggcagatcg tatgtaatat
    13321 gcattataaa acctcttcgc ctgaattaaa tatgtaggta accgtaaccg acacctgcaa
    13381 gacagacagg tggattattt atgtttaaat aaaaacaaat aactaaccaa cttctattaa
    13441 ttgccttcgt ctacacccat tataagatga atactctttg gctaattcca ccaaaaccga
    13501 tgctgctttt ttaatacgat aatttttcga ccataccgcg gcatattgtg ccacaggtaa
    13561 tgtttcttca accggaatag taataaattg attagaacca aaaggtgacg tcatatcaca
    13621 aggaattaca gttaagaaat cagcattgag aacaagatta taaattgtca cgactgagtc
    13681 ggttttaacg atgttttcaa tactgatgcc atttctttgt aacgtagtaa gcagttcgct
    13741 gtagtacccc atattagttt gtggcaacac ccactgttcg ttcttcaacg actccagcgt
    13801 ggtggtgccg gtgcatgttc gggacttact ggctaccagc acaaactcgg actcgaacag
    13861 cggctcaaca tgtaaatcct gaagcttcat ttctgcactt aacgtaccaa tcgcaaaatc
    13921 cagtcgaccg tcgcggattg ccggtaagaa cgaagacagt tgcgcttcat acatagaaac
    13981 ctgcgctttc gggaacacct ctttgaactt gttgatcatc cctgacataa aagtaaaacc
    14041 aatcaatgaa ggaaaaccaa atgagacttc caccaccgcc tcagaagaca taccgcttat
    14101 ctcattaacc atatttttca tttcacgggt aatggattcg gaacgggaga gtaacaattg
    14161 accggcaggt gttaatgtta caccggtatt tttccgcacc actaattcca caccaaaata
    14221 atcttcaata tcgttaatga ttttactgac ggccggttga gttaacccta attcttttgc
    14281 agccgagccg atagaaccac ttctaatgac ttcctgaaag actaccaggt gctgcgtttt
    14341 cggaagaaga atagtgctca taacgaccta cgttaattac ctcattgacg gcatgaagtg
    14401 tatcaaaatg aaatgaacag gatatgtgcg accactcaca aattaacttt caatactttc
    14461 cagagtatcg ttattacaat taaatacctt taaatatcaa caagttaaag tataaaaatc
    14521 agcataaacc ctga
//
LOCUS       DBANUU010000111        14457 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000111 DBANUU010000000
VERSION     DBANUU010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.111"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(144..2411)
                     /gene="adiA"
                     /locus_tag="ACIE68_003451"
     CDS             complement(144..2411)
                     /gene="adiA"
                     /locus_tag="ACIE68_003451"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFX5909713.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHSDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2610..3518)
                     /gene="melR"
                     /locus_tag="ACIE68_003452"
     CDS             complement(2610..3518)
                     /gene="melR"
                     /locus_tag="ACIE68_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFX5909714.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3801..5156
                     /gene="melA"
                     /locus_tag="ACIE68_003453"
     CDS             3801..5156
                     /gene="melA"
                     /locus_tag="ACIE68_003453"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFX5909715.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5259..6680
                     /gene="melB"
                     /locus_tag="ACIE68_003454"
     CDS             5259..6680
                     /gene="melB"
                     /locus_tag="ACIE68_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFX5909716.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6819..7448)
                     /gene="yjdF"
                     /locus_tag="ACIE68_003455"
     CDS             complement(6819..7448)
                     /gene="yjdF"
                     /locus_tag="ACIE68_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFX5909717.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7571..9217)
                     /gene="fumB"
                     /locus_tag="ACIE68_003456"
     CDS             complement(7571..9217)
                     /gene="fumB"
                     /locus_tag="ACIE68_003456"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFX5909718.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9295..10635)
                     /gene="dcuB"
                     /locus_tag="ACIE68_003457"
     CDS             complement(9295..10635)
                     /gene="dcuB"
                     /locus_tag="ACIE68_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFX5909719.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11206..11925)
                     /gene="dcuR"
                     /locus_tag="ACIE68_003458"
     CDS             complement(11206..11925)
                     /gene="dcuR"
                     /locus_tag="ACIE68_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFX5909720.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11922..13553)
                     /locus_tag="ACIE68_003459"
     CDS             complement(11922..13553)
                     /locus_tag="ACIE68_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFX5909721.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13734..13964
                     /gene="yjdI"
                     /locus_tag="ACIE68_003460"
     CDS             13734..13964
                     /gene="yjdI"
                     /locus_tag="ACIE68_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFX5909722.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            13976..14248
                     /gene="yjdJ"
                     /locus_tag="ACIE68_003461"
     CDS             13976..14248
                     /gene="yjdJ"
                     /locus_tag="ACIE68_003461"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFX5909723.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
BASE COUNT         3468 a         3468 c         3775 g         3746 t
ORIGIN      
        1 aaaaaaagcg cggggtgggg ccgcgcaaaa agtatgacat ttttgtttgc agcaattttt
       61 atgaaaacgt tgccatactc aaaaggccta aggccaggcg gcgtaatgtt atttaaacaa
      121 ttacgccttc agcggaatag tggttacgct ttcacgcaca taacgtggta aataccgtca
      181 ataatttcag tcccttcagt ttcgtgttca aatccaggga aatggtggtc ccaggattgc
      241 agcgagcgta aataacttac ttgcggactg tttttatcgc cgaagttttc accagacagc
      301 agcatcggga ttcctggcgg atacgggata actgagtttg ccgcgatgcg tcctggcaga
      361 ttttcaatgg ataccagttc gacattgttg tcgacaatcg cgttgtacgc ttcacgcggg
      421 gtgatttccg ccatcggcag gccggaatag gcttcgttca accgtgcgcc agggttgttt
      481 tctttcagcc aggcaaacat ggtgtcaccc agatcgtgaa tccccatgtt cgcgtaagtg
      541 tcaggatatt gttcaacaag ttccggcatc acctgcgcca gcggtgtgtt ggcgtcatag
      601 tggcgtttga aggagcaaag ggtgttaacc agagtccccc atttcccacg ggttacgccc
      661 atagagaaca ggaacataat ttggaagtca gtggtgcggg taggcacaat gccgtggcga
      721 ccaagccagg cagtgaccag cgccgccgga acaccggttt cttccagttc accatcttca
      781 cccattcccg gagcaaggat gctgacttta attgggtcga gcatactcca gttatccgga
      841 atatctttga agccgtgcca gctttcgccc ggatgcatta cccagcagtc ctgaacggtg
      901 gtcagcagtt tggttggtgc gtcagcaaag tcataggttt tgccggtttg tgggtcggtg
      961 acgacttctt tgttccacgg tttgaagaac cagctaccgt cagcggtgaa ctctttatat
     1021 agccgcgcca tcgcctgacg gaaatcaacc gcttcgtcaa tcacttcctg tgtcagtgac
     1081 aggccgctgt tgccgtccat catcgacacc gccacgtcgt tggatgcgca gatggcatac
     1141 agcggggagg tggtggcatg catcatgtag gcctggttga agcgggagaa gttaatcgcc
     1201 ccacgacctt cacgtacatg aatataagaa gcctgtgaca gcgcattcag cagtttgtgg
     1261 gtggagtggg tggcgaaaac ggtaggaccg ttgtgatcgc caggttcgcc gcgcatggca
     1321 tagtgatcgg catagatcgg gttgaaacgt gcatagccgt accaggcttc gtcaaagtgc
     1381 agacgatcgg aggttttttc cagcagatcc tgcgcttctt tagcgttata acacacgccg
     1441 tcataggtgc agttggtcac cacgcagtaa gacggttttt gcccggcttt gtctttggtc
     1501 agcgggcttt cactgatttt cttctgcaag gtttcaggtt gcatttcctg cggatagatt
     1561 ggcccgataa tgccgtagcg gttgcggctt ggcaccatat agaccggttt cgcgcctgtc
     1621 agcatcaagc cttgttcgat ggatttatgg caattacggt caacgaccac gacatcgtta
     1681 tcggtcatgc aagcctgcat gatggtgcgg ttagagccgg aagtaccgac gactaccgac
     1741 caggagcgat cggcaccaaa tacgcgtgcg gcatattttt cgctttcgcc aaatgcgcca
     1801 gtatggtcaa gcaaagaacc gagggaagtt cgttcgatgc ccatgtcggt gcggaacaga
     1861 ttttcaccat agtagtcatg gtagaaacgt ccggcgggtg ttttggtaaa accaacgccg
     1921 ccctggtggc ctggcgctgc ccaggaatat tcatggatgt cactatattt catcagcgcg
     1981 ctgaacagtg gcggcaacag ctgctggcgg tagcgggtca tcgcggcaac ggcgcgtccg
     2041 gcgataaagt cggcggtatc ttccagaatc caggcgaatt catcgacaag ctccagcaga
     2101 tcccggtcca ttgcggcgag ggctttttcc cgatcgccca acaggaagac cggcacgttt
     2161 tgttggcgct catgaagctt accgatcaat tgtctgacgt tttgatgttc gtccgaatgt
     2221 tccatttgat agctgaacat caggcagtca atggcttcgt ttgaagagag aatggcaaaa
     2281 ccatcatcaa aggaggtgga tttaatcacg gtaacatttt gctggcttaa agcatctgcc
     2341 agacgctcaa cggcgttacc gacccaggtg tcttgatgga gaaactcgct ttcaacaatt
     2401 aatactttca tcattgctta cccggttatg aaggaatctt cgttgcgggc aagtatcttc
     2461 cggccttttc gggctgtaaa gcgcgtgaaa aacaaacatt tttgaaatat ttagttttat
     2521 aaacaatacg ttatgtgatt attttaaatt attttcactg cttattatcg acggctaaac
     2581 tatttttttg gctgatactg atatcgtctt tagccgggaa acgtctggcg gcgctgttgg
     2641 ctaagtttgc ggtattgttg cggcgacatg ccgacatatt tgccgaacgt gctgtaaaaa
     2701 cgactacttg aacgaaagcc tgccgtcagg gcaatatcga gaatactttt atcggtatcg
     2761 ctcagtaacg cgcgaacgtg gttgatgcgc atcgcggtaa tgtactgttt catcgtcaat
     2821 tgcatgaccc gctggaatat ccccattgca tagttggcgt taagtttgac gtgctcagcc
     2881 acatcgttga tggtcagcgc ctgatcatag ttttcggcaa taaagcccag catctggcta
     2941 acataaaatt gcgcatggcg cgagacgctg tttttgtgtg tgcgcgaggt tttattgacc
     3001 agaatcggtt cccagccaga gaggctaaat cgcttgagca tcaggccaat ttcatcaatg
     3061 gcgagctggc gaatttgctc gttcggactg tttaattcct gctgccagcg gcgcacttca
     3121 aacgggctaa gttgctgtgt ggccagtgat ttgatcacca tgccgtgagt gacgtggtta
     3181 atcaggtctt tatccagcgg ccaggagaga aacagatgca tcggcagatt aaaaatcgcc
     3241 atgctctgac aggttccggt atctgttagt tggtgcggtg tacaggccca gaacagcgtg
     3301 atatgaccct gattgatatt cactttttca ttgttgatca ggtattccac atcgccatcg
     3361 aaaggcacat tcacttcgac ctgaccatgc cagtggctgg tgggcatgat atgcggtgcg
     3421 cgaaactcaa tctccatccg ctggtattcc gaatacagcg acagcgggct gcgggtctgt
     3481 ttttcgtcgc tgctgcacat aaacgtatct gtattcatgg atggctctct ttcctggaat
     3541 atcagaatta tggcaggagt gagggaggat gactgcgagt gggagcacgg ttttcaccct
     3601 cttcccagag aggcgagggg agcgtccgag catcatgagg ccgaaaactc tgcttttcag
     3661 gtaacttatt accataaact cagatttact gctgcttcac gcaggatctg agtttatggg
     3721 aatgctcaac ctggaagccg gaggttttct gcagattcgc ctgccatgat gaagttattc
     3781 aagcaagcca ggagatctgc atgatgactg cacccaaaat tacatttatc ggcgctggtt
     3841 cgacgatttt cgttaaaaat attcttggtg atgtgttcca tcgcgaggcg ctgaaaacgg
     3901 cgcatattgc cctgatggac attgatccca cccgcctgga agagtcgcat attgtggtgc
     3961 gtaagcttat ggattcagca ggggccagcg gcaaaatcac ctgccacacc caacagaaag
     4021 aagccttaca ggatgccgat tttgtcgtgg tggcatttca gattggcggt tatgaacctt
     4081 gcacggtgac tgatttcgag gtctgtaagc ggcatggtct ggaacaaacc attgccgata
     4141 cgttggggcc gggcggtatt atgcgcgcgc tacgtaccat tccgcatctg tggcaaattt
     4201 gcgaggacat gacggaagtc tgccccgatg ccaccatgct caactatgtt aacccaatgg
     4261 cgatgaatac ctgggcgatg tatgcccgct atccgcatat caaacaggtc gggctgtgcc
     4321 attcggtgca gggaacggcg gaagagctgg cgcgtgacct caatatcgac ccagctacgc
     4381 tgcgttaccg ttgcgcaggt atcaaccata tggcgtttta cctggagctg gagcgcaaaa
     4441 ccgccgacgg cagttatgtg aatctctacc cggaactgct ggcggcttat gaagcagggc
     4501 aggcaccgaa gccgaatatt catggcaata ctcgctgcca gaatattgtg cgctacgaaa
     4561 tgttcaaaaa gctgggctac ttcgtcacgg aatcgtcaga acattttgct gagtacacac
     4621 cgtggtttat taagccaggt cgtgaggatt tgattgagcg ttataaagta ccgctggatg
     4681 agtacccgaa acgctgcgtc gagcagctgg cgaactggca taaagagctg gaggagtata
     4741 aaaacgcctc ccggattgat attaaaccgt cacgggaata tgccagcaca atcatgaacg
     4801 ctatctggac tggcgagccg agtgtgattt acggcaacgt ccgtaacgat ggtttgattg
     4861 ataacctgcc acaaggatgt tgcgtggaag tagcctgtct ggttgatgct aatggcattc
     4921 agccgaccaa agtcggtacg ctaccttcgc atctggccgc cctgatgcaa accaacatca
     4981 acgtacagac gctgctgacc gaagctattc ttacggaaaa tcgcgaccgt gtttaccacg
     5041 ccgcgatgat ggacccgcat actgctgccg tgctgggcat tgatgaaata tatgctcttg
     5101 ttgacgacct gattgccgcc cacggcgact ggctgccagg ctggttgcac cgttaaaacg
     5161 cgactaaacg ctactgcgcc gggggattta ttccggcgca cacctctgac gataccaata
     5221 acagaaggcg ggcgttggta acagcgaccc gataccctat gagcatttca atgactacaa
     5281 aactcagtta tggatttgga gcgttcggga aggattttgc gatcggcatt gtgtatatgt
     5341 acctcatgta ttactacacc gatgtcgttg ggctgtctgt gggtttggtc ggtactttgt
     5401 ttctggtggc gaggatctgg gatgctatta acgatccgat tatgggatgg attgtaaatg
     5461 ctacgcgatc gcgatggggt aagttcaaac cctggatcct gatcggtacg ttggcaaact
     5521 ctgtaatctt atttctcctc tttagtgcgc atctgtttga aggtactact cagattgtct
     5581 ttgtttgcgt gacctacatc ctctggggca tgacttacac cattatggat attcccttct
     5641 ggtcgctggt tccaaccatc acgctcgata aacgtgagcg cgaacaactg gttccttatc
     5701 cgcgtttttt tgccagtctg gcaggctttg ttacggcagg tgtgacgcta ccatttgtta
     5761 attatgtcgg cggtggcgat cggggatttg gctttcagat gttcactctg gtactaatcg
     5821 ccttttttat tgtttcaacc atcatcactc tgcgcaatgt gcatgaagtc ttttcgtcag
     5881 acaatcaacc gtctgctgaa ggaagccatc tgacacttaa agccatcgtt gcgctaattt
     5941 ataaaaacga tcagctttca tgcctcttgg gtatggctct tgcttataat gtagccagca
     6001 acattattac cggatttgct atctattatt tctcatatgt tatcggtgat gcggatttgt
     6061 tcccctatta tctgtcgtat gcgggagctg ctaacctggt gacgttagta ttcttcccac
     6121 gcttagttaa atcattatcc cgacgcattt tatgggccgg agcatctatt cttccggtgt
     6181 taagctgtgg tgttctcctg ttaatggcat taatgagcta tcacaacgtc gtcctcattg
     6241 tgattgcggg tattttgctg aatgtgggaa cggcgctttt ctgggtatta caggtcatca
     6301 tggtggcaga taccgttgat tacggtgaat ataaactgca cgtacgctgt gaaagtatcg
     6361 cttactccgt gcagactatg gtggtgaagg gcggttcagc ctttgcggct tttttcattg
     6421 cggttgtgtt agggatgatt ggctatgtac cgaatgttga acagtctacg caagccctat
     6481 taggtatgca gtttattatg attgctctac caactctgtt tttcatggta acgctgattc
     6541 tctacttccg tttctatcgc ctcaatggcg acacgctgcg caggatccag attcatctgc
     6601 tggataaata tcgcaaagta ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag
     6661 tgagtgatgt gaaagcctga cgtggacaat agctaacgaa aatggcctga tgcgatatgt
     6721 ttatcaggcc acgtcttaat gtaatgcttt ggatttgcaa attttgtaga ccggataagg
     6781 aattcacgcc gcatccggca tcaacaaagc gcacgttgtt atccggttat caagccaaag
     6841 cgccgtagct ggcggcaatg aaagcgagcg aggaatatca ccgtcgttaa tgcgccaagc
     6901 agcgcgcaga acatatcgga ttgcgtgtcc cactggtcgc cctgggtgcc gagaaaatca
     6961 tccgctccct ggcccattgc cagcgccgcc caccactcga ttaattcata catcgcgctt
     7021 atcgccagcg ccacgcagca gaccaggaac gccaccattt tacgtccgcg cacgtacatc
     7081 ccgcgcacga gaatttctcg tgccaccagt gcaggcacca gcccctggaa aaaatgcccc
     7141 agcttgtcat acggattacg gctcaacccc agccattcct gcacctcaaa accgatgggg
     7201 actttcgcgt aggtgtattg cccgccgacc atcaggatga tggcgtgaaa gaaaatgagc
     7261 gtatagagca gcggcgttaa cggataacgt ttagcggtgg caagcagtag cggcacgaca
     7321 ataatcaccg gtgtcacttc catcagccag gtgagtttgt catgggccga aatgccggta
     7381 taaatcagga ttaacgttag cgccagtgcg ccggtgttaa gaattaacgg cttgagtgtg
     7441 cgggtcatgg tgagttcgca agtcagggaa aatactgact attcaccagc aagcgtgaaa
     7501 ttacaatcgc aaaagaggtg gcccaggggg atcacctggc agcatgctgc caggcgctgg
     7561 gccgaagagg ttacttagtg cagttcgcgc actgtttgtt gacgatctgc tggaagaagt
     7621 cgttaccttt gtcatcgacc aggataaacg ccgggaaatc ttctacttcg attttccaga
     7681 tagcttccat acccagctcc ggataagcga cgcattccag atgcttgatg ctctgctgcg
     7741 ccagtaccgc cgccggaccg ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg
     7801 cgtcggtaac ctgctgactg cggttacctt tcgccagcat gatcatgctg ccgccgtggg
     7861 attgcagcag atccacgtag gagtccatac ggcctgcggt ggttgggcca agtgaacctg
     7921 atggataacc ggcaggggtt ttcgccggac ccgcgtagta gatcgggtga tctttgatgt
     7981 actgcggaag ttctttaccg gcgtcaatca gctctttcag cttggcgtgt gcaatatctc
     8041 ggcccacgat aatggtgccg gtgagcgaca aacgagtgga taccgggtat tgcgaaagct
     8101 gggcgaggat ctctttcatc gggcggttaa ggtcaacttt caccgcttcg ccttcaccgg
     8161 cctggcgcag ttcttgtgga atgtactggc ctgggttgtg ttccagtttt tcgatccaga
     8221 taccttcgcg gttgattttc gctttaatgt tacggtcagc ggagcaggag acgcccatgc
     8281 cgaccgggca ggatgcgccg tgacgtggca gacggataac gcgaatgtcg tgcgcgaagt
     8341 atttaccgcc aaactgcgcg ccaagaccga gtttctgggc ctcttccagc agttcctgtt
     8401 ccagttggac atcgcggaac gcctgaccat gttcgttccc ttccgtcggc agttcatcgt
     8461 aatagtgagc gcttgctaac ttgacggttt tcaggttggt ttccgcagac gtaccgccaa
     8521 tcacaaacgc gatatggtac ggcgggcagg ctgcagtacc gagggtacgc attttctcga
     8581 cgaggaagtt tttcagtttg ccgggagtca gcagggcttt ggtttcctgg tagagatacg
     8641 ttttgttggc agagccgccg cctttcgcaa cgcaaaggaa tttgtactca tcgccatcta
     8701 ccgcgtacag gtcgatttgc gcaggcaggt tagtgccggt gttgacctct ttgtacatgt
     8761 ccagcgccgc attctgtgaa tagcgcaggt tatcttcgat ataggtgtta tagacgcctt
     8821 tcgacagcgc ttcttcatca ccgccgccgg tccacacgcg ctggcctttt ttaccgacga
     8881 tgatcgcggt gccggtatcc tggcaggtcg gcagcacgcc tttggcggcg atttcggagt
     8941 ttcttaagaa ttgcagcgcc acgtacttgt cgttttcgct ggcttctgga tcgtgaagaa
     9001 tagccgcaac ctgtttctgg tgtgccgggc ggagcataaa agaagcgtcg tgaaaggctt
     9061 gctgcgccag cagggtcagg gcttctggtt ccactttcag gatggtttcg ccgtcgaagt
     9121 cggcaacgct aacgtaatcg gaagtgagta gatagtattc ggtattgtct ttccccatcg
     9181 ggaaaggtgc ctggtagata aagggtttgt ttgacatagc ttccagcctg taactctgta
     9241 tttgttattc gaaaatggcg tgccgcgtag tcacgggcgg cacgcaaagt gcatttataa
     9301 gaacccgtac atcgcggcga agatccagcc gaagacgcac gatacgctca caccaatcaa
     9361 ccccggcaga ataaagctgt ggttgatgac gaagcgaccg atgtgggtgg tgccggaacg
     9421 gtcaaactga atcgctgcca gatcgctcgg ataagtcggc aggatgtaat aaccgtagca
     9481 agccggtgct gaagccacga tgtatgccgg atcaacgccg atcgccagcg cgaccggaac
     9541 aatcgccgcc agcgccgcag cctgagagtt tacaaacttg gaaaccagca gcagaacaat
     9601 ggcataggcc cacggatact ctttcaccat ttcacccagt acgccctgaa tttcagacat
     9661 atgcgcaccg aacatggttt ctgccatcca tgcgataccg tacaccgcca cgatggcgat
     9721 cataccggaa cggaagactt cgttttttga gatagacgcg ggattggttt tggtcaggat
     9781 aataatcagc gccccggtca gcagcataaa catctgaata accagtacca tcgacagcgg
     9841 tttgccgccg aaggatggac gcaggtccga atcagcacca agaagggcga ctacagcgat
     9901 tgcaccgagg aaaatccaca ttgccagcca gttgcttttc ggcagttttt tatcgagcag
     9961 cgtcgcggta tcaccgtaaa catactcacg gttttccggt acggagatga atttctggaa
    10021 ctcttcgtct ttatccagat ctttaccgcg gaaccagctg aagataccga tcgccaggat
    10081 accgattaac gtcgatggaa tggtgattgc cagcaggtcg aggaactcaa gatggcgacc
    10141 atcaaaggtg acattaccca gcattgcaac cagagacacg accgcaaccg acaccggact
    10201 ggcgataatc cccatctgtg caccgataga acttgccgcc atcggacgtt ccggacggat
    10261 gttgttctta atggcgacgt cgtagatgat cggcagaatg gtgtaaacca catgacccgt
    10321 accgcaaaga atggtcagtg tacaggtcac aaacggcgcg acaattgaga catatttcgg
    10381 gttgcggcgc agcaacttct cggcaatttg cagcatgaca tcaagaccgc ccgaagcttg
    10441 caaggtcgcc gatgccgcca ccaccgcaat gataaccagc atgacatcaa ctggtggttt
    10501 acctggctga aggtggaaga cgaagaccag aatgaccaga ccgataccgc ctaataaacc
    10561 cagcgcgata ccaccctttc tggcaccata aaacagacat atcagtatta tgataagttg
    10621 gatagtaaat aacatgtgtg aaccctcgcg ataatcctat ttaaattttt gctgaataga
    10681 tcacagtcac gttctgtttt gtatgaactg ttttcagagc ctgactaaat atccgtttgg
    10741 tcgttaccgg ctttagcaaa tacctcacag taaatattgg ctgattgaag cagcggcaaa
    10801 tttatgcact gtgataagcg gctttttcag agagaaaaac tctctggcga tgggtttata
    10861 gtttgtactg cgcagatgtt aatgttttgt ttgctgttat cttattgata tttatcggtc
    10921 tattttttgt tctcagggca gatatcatta ggtttaaaga ttaatctgat ctacccattt
    10981 gtgggtaaaa atacacataa tgcgggtgac ataatagtta attaactttt gttagcgttt
    11041 tgaaattaaa aacaccgttc acctgaagag atattaattt ttagcgatga tggagggata
    11101 attatatttg atctggcaca agttttactg atgaaggatg taacttgtgc caggggtatt
    11161 ttgcattacg gtaataatta ttacttacag ataaccagcg atccgttatt ggcaatattg
    11221 tttcagtagt gagtagtgtt ctgcctgaat acggtaacgg taaaccggac gccccgtgac
    11281 gccataatgg atactggtga acaagatgtg gcagttgacc agccagatga ggtatttacg
    11341 gcaggaaaca cgcgagatgt taacctcgtt agccagctcg tcggttgaaa actcatagtc
    11401 ctgatgcgcg tcaatccact ggcacagcgt gcgtaacgtc tgcggcgtta agccttttgg
    11461 caagcgacga ggatcctgtt cgttggagct gctgccgtgg attagctgat caagctcggc
    11521 ctggtcataa tactgatgtt tttccagcgc cattttcttt tgccgccagc cggtgagcgc
    11581 ctcttcaaag cgggaagcct ggaagggttt gatcaggtaa tccacgacac cgtaatgcag
    11641 cgaatcttta atggttgccg catcggctgc ggaggagatg acaatcacat cacttttgca
    11701 acgcgcgtta tgcaggacag gcagtaaatc gagcccgttc tctttttgca tatagatatc
    11761 gagcaatatc aggtcgatag gcgtatcgct attgaagata atctctttgg ctttctccag
    11821 cgtcgaggct gttccacagc attgaaagcc tgggatttgt gctacgtatc ggcgattcag
    11881 ctccgcgacc attgcgtcgt catcgataat taatacattg atcatctgtt cgacctctcc
    11941 ccgtcccagg gtatctggac aaaaaattgt gtgaaaatcc cgggttccga ttccacggcg
    12001 atgctgccgc cgagattttc tacctgttgt ttgacaagtg ctaaaccgac gcctcgctcg
    12061 cttccttttg tcgagacacc tttgtcaaaa atgtgatcga ttttgtcggg tgcgatcccc
    12121 ggtccatcat cgttaacttc gcagtgcagc cagccgtgac ggtagtgcaa tgttacgcta
    12181 atttcgccac cgggttccgg ccctaatgct tccagcgcgt tttctatcag atttcccaat
    12241 gtggtaatca gcgtcgcgac ctggtcctca ctgccgctgt ctggcagctg gctttcactg
    12301 tttaaaatca gcgtatggcc taaatcggtc gcgcgattaa tcttgctgat taaaaaacca
    12361 gcgataaccg gagatttgat cttacccagc agagagccaa tctcttcctg atagttattg
    12421 gctgttttga gaatgtaatc ttccaactgc ttataactct tcagatgcaa taatccgaga
    12481 atcacatgca atttattcat aaattcgtgg gatcgttcac gaagtgcatc agcatagttg
    12541 accagaccgt cgagtcgctg catcagttta cgaacttcag ttttgtccct gaaggttgaa
    12601 atggcaccga tgataacgcc attactgcgc accggaacgg tgttgatcag taatagccgg
    12661 tctttaatcg taatctcttc gtcgcggcgc ggcgtaccgt cgcgtaacac ttccgagaca
    12721 tctaccacct gtgaccatga gtggctgagc gtcgacagtt tctcatcgtc ctgcgactta
    12781 cggtaattca gcaattcttg tgcggcatcg ttgatcagcg tgacctcgcc gcgatcgtcc
    12841 acggcaacga cgccttcttt gatagactgc aacatggcct ggcgctgctc aaacagcgtg
    12901 gagatttcgt agggttccag gccgaaaagg atttttttca gtaccttaac cagaatgcag
    12961 gtgccaatca gtccgaccag catgccaaat aataccgacc agataatgct ccagcgactg
    13021 tcattgattt gttgggtcac gcggcttaac tcaaggccga tcgccaccac gccaatttgt
    13081 ttatgatttt catcgtagat gggggtaaat acgcgtaaag cctgcgccag aaaaccgcga
    13141 ttgatagcga cattttcttt gccattcagc gctttaagga tgtcatcacc tttaaatggc
    13201 tgaccaatac gctgggcttc aggatgcgag tagcgaagac tttgcatatc ggtaacgaca
    13261 ataaacagca gatcgttgcg tttgcgcacg gcttccgcga tggcctggat tccgctctcc
    13321 tgcggttttt tctgcaagcc ctgacggatt tccggcgagt cggcgagggt acgcgccact
    13381 gccagtgcct tgttggccag cccgtctcgc gtcatatcac tgatttgcga gaagtaaatc
    13441 agatgcacca ccaatagcac cgagaacagt accgcgctga ccattaagat cactgtggta
    13501 ctcaatttca tcggacgttt gcgtaacatg cggtagggca atgaatgtct catcagcttc
    13561 cttgtgtgac aaatttctta agcattatct ctgatgaggc gggtaattca aagggagtaa
    13621 gaatgattgg ctatataggg gaagagactc tggcaacgga aactgccagt gctgtatgaa
    13681 gattccaggg ctatgcttat agcgataatc atactgatga gagagggaaa gtcatggatc
    13741 aggcgctact ggacgagggt tatcgctgtt ataccggcga aaagatcgat gtctatttca
    13801 acactgcgat atgtcagcat tctggcaatt gcgtacgtgg caacggcaag ttatttaatc
    13861 tcaaacgaaa gccgtggatc atgccggatg aagtcgacgt cgtcactgtg gttaaagtga
    13921 ttgatacgtg cccgagcggc gcgctgaaat accgtcataa ataagcgagg gtaaaatgga
    13981 aatacgcgaa ggccacaata aattttacat taatgatgaa cagggcaagc aaatcgctga
    14041 aattgtcttt gtgccgaccg gagagaattt agcgattatc gaacataccg atgtcgatga
    14101 aagcctgaaa gggcaaggga ttggtaaaca gctggttgcg aaagtcgtgg aaaaaatgcg
    14161 tcgggaaaaa cgaaaaatta tcccattatg tccatttgcg aaacacgaat ttgataaaac
    14221 gcgggagtat gatgatattc gcagttgatg ggagagtaca gattcacgat atttttcatg
    14281 ctctctgcgg tgtgatgcag gagagcatct gaagggtagg gggatgcaca aagaatgggc
    14341 agagagcgcg tttttttgtc ccaagtcatc ccctttactg agcaaaaaaa agaatatctc
    14401 ctatatgaga atcatcaatc ggggttaata agttttgcgt ccccagagtg ttcaata
//
LOCUS       DBANUU010000112        14177 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000112 DBANUU010000000
VERSION     DBANUU010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.112"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            162..1220
                     /gene="citC"
                     /locus_tag="ACIE68_003462"
     CDS             162..1220
                     /gene="citC"
                     /locus_tag="ACIE68_003462"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFX5909724.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1235..1531
                     /gene="citD"
                     /locus_tag="ACIE68_003463"
     CDS             1235..1531
                     /gene="citD"
                     /locus_tag="ACIE68_003463"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFX5909725.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1528..2436
                     /gene="citE"
                     /locus_tag="ACIE68_003464"
     CDS             1528..2436
                     /gene="citE"
                     /locus_tag="ACIE68_003464"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFX5909726.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2447..3979
                     /gene="citF"
                     /locus_tag="ACIE68_003465"
     CDS             2447..3979
                     /gene="citF"
                     /locus_tag="ACIE68_003465"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFX5909727.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3983..4534
                     /gene="citX"
                     /locus_tag="ACIE68_003466"
     CDS             3983..4534
                     /gene="citX"
                     /locus_tag="ACIE68_003466"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFX5909728.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4509..5387
                     /gene="citG"
                     /locus_tag="ACIE68_003467"
     CDS             4509..5387
                     /gene="citG"
                     /locus_tag="ACIE68_003467"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFX5909729.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5438..6901
                     /gene="citT"
                     /locus_tag="ACIE68_003468"
     CDS             5438..6901
                     /gene="citT"
                     /locus_tag="ACIE68_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFX5909730.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7015..7821
                     /gene="rna"
                     /locus_tag="ACIE68_003469"
     CDS             7015..7821
                     /gene="rna"
                     /locus_tag="ACIE68_003469"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFX5909731.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8051..8461
                     /gene="rnk"
                     /locus_tag="ACIE68_003470"
     CDS             8051..8461
                     /gene="rnk"
                     /locus_tag="ACIE68_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFX5909732.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8599..8688
                     /gene="yldA"
                     /locus_tag="ACIE68_003471"
     CDS             8599..8688
                     /gene="yldA"
                     /locus_tag="ACIE68_003471"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFX5909733.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8692..9930)
                     /gene="ybdR"
                     /locus_tag="ACIE68_003472"
     CDS             complement(8692..9930)
                     /gene="ybdR"
                     /locus_tag="ACIE68_003472"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFX5909734.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10151..10579
                     /gene="uspG"
                     /locus_tag="ACIE68_003473"
     CDS             10151..10579
                     /gene="uspG"
                     /locus_tag="ACIE68_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFX5909735.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10700..12265)
                     /gene="ahpF"
                     /locus_tag="ACIE68_003474"
     CDS             complement(10700..12265)
                     /gene="ahpF"
                     /locus_tag="ACIE68_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFX5909736.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12436..12999)
                     /gene="ahpC"
                     /locus_tag="ACIE68_003475"
     CDS             complement(12436..12999)
                     /gene="ahpC"
                     /locus_tag="ACIE68_003475"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFX5909737.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13370..14116
                     /gene="dsbG"
                     /locus_tag="ACIE68_003476"
     CDS             13370..14116
                     /gene="dsbG"
                     /locus_tag="ACIE68_003476"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFX5909738.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3366 a         3748 c         3577 g         3486 t
ORIGIN      
        1 cacgcagaaa tactccttag gatgtatagc gaaaggagaa aaagatatac ctcgatcacc
       61 cccctttctc ccaagtgaaa ataaaaggtt atcagtttgc aacattgaac aacattcgtt
      121 gcaaatcgat aacaacatgc accttcagga tactatttat tatgttcggc aatgatattt
      181 tcacccgcgt aaaacgttca gaaaataaaa aaatggcgga aatcgcccaa ttcctgcatg
      241 aaaatgattt gagcgttgac accacagtcg aagtatttat taccgtaacc cgcgatgaaa
      301 agcttatcgc gtgcggtgga attgccggaa atattattaa atgcgttgcg atcagtgaat
      361 ccgttcgcgg tgaaggactg gcgctgacat tagccactga actgataaac ctcgcctatg
      421 agcggcacag cacgcatctg tttatttata ccaaaaccga atacgaggcg ctgttccgcc
      481 agtgcggttt ttccacgctg accagcgtac ccggcgtgat ggtgctgatg gaaaacagcg
      541 ccacgcgact gaaacgctat gccgaatcgc tgaaaaaatt tcgtcatcca gggaacaaga
      601 ttggctgcat tgtgatgaac gccaatccct tcacgaatgg tcaccgttat ctgattcaac
      661 aagctgcggc acagtgcgac tggttgcatc tgtttttagt caaagaagat tcttcacgct
      721 tcccctatga agaccggttg gatctggtgt taaaaggcac cgccgatatt ccacgcctga
      781 ctgtgcatcg cggctccgaa tacatcatct cccgcgctac gttcccttgc tacttcatta
      841 aagaacagag cgtcattaac cattgttaca ccgaaattga tctgaagatt ttccgtcagt
      901 acctcgctcc cgcgctgggt gtaactcacc gctttgtcgg tactgaaccc ttttgtcgcg
      961 ttaccgccca gtacaaccag gatatgcgct actggctgga aacgccgact atctccgcac
     1021 cgcccatcga actggttgaa attgagcggc tgcgttacca ggagatgccg atatccgctt
     1081 cccgggtacg tcaactgctg gcgaaaaacg atctcacggc tatcgcgccg ctggtccctg
     1141 cagtcacgct gcattatttg cagaacctgc ttgagcactc ccgccaggac gcggcagctc
     1201 gtcaaaagac ccccgcatga gaaacaggtg aaaaatgaaa ataaaccagc ccgccgttgc
     1261 aggcaccctt gagtctgggg atgtgatgat acgcatcgcc ccactcgata cgcaggatat
     1321 cgacctgcaa atcaatagca gcgttgagaa acagtttggc gatgcaattc gcaccaccat
     1381 tctggacgtt ctcgcccgct acaacgtgcg cggcgtacag ctgaatgtcg atgataaagg
     1441 cgcactggac tgcattttac gtgcacgact ggaagccctg ctggcacgcg ccagcggtat
     1501 cccggctctg ccatgggagg attgccaatg atttccgctt cgctgcaaca acgtaaaact
     1561 cgcacccgcc gcagcatgtt gtttgtgcct ggtgccaatg ccgcgatggt cagcaactcc
     1621 ttcatctacc cggctgatgc cctgatgttt gacctcgaag actccgtagc attgcgtgaa
     1681 aaagacaccg cccgccgcat ggtttaccac gcgctgcaac atccgctgta tcgcgatatt
     1741 gaaaccattg tgcgtgtcaa cgcgctggat tccgaatggg gtgtcaacga cctggaagcc
     1801 gtcgttcgcg gtggtgcgga cgttgtgcgt ctgccgaaaa ccgataccgc tcaggatgtt
     1861 ctggatatcg aaaaagagat cctgcgtatc gaaaaagcct gtggtcgtga acccggcagc
     1921 accggcctgc tggcggcgat tgaatctccg ctgggcatta cccgcgcagt ggaaatcgct
     1981 cacgcttccg agcgtttgat cggtatcgcc ctcggtgcag aagactatgt gcgcaacctg
     2041 cgtacagaac gctccccgga aggaactgaa ctgctgttcg cacgctgttc cattttgcag
     2101 gccgcgcgct ctgcgggtat tcaggcgttc gataccgtct attccgacgc taacaacgaa
     2161 gccggattcc tgcaagaagc cgcccacatc aaacagctgg gctttgacgg caaatcgctg
     2221 atcaacccgc gtcagattga tctgctgcac aacctctacg caccgaccca gaaagaagtg
     2281 gatcacgccc gccgcgtcgt agaagccgct gaagccgccg ctcgcgaagg cctcggcgtg
     2341 gtttccctga acggcaagat ggtggacggt ccggttatcg atcgcgcccg tctggtgctc
     2401 tcccgtgcag aactttccgg catccgcgaa gaataaggca atcaaaatga cgcagaaaat
     2461 tgaacaatct caacgacaag aacgggtagc ggcctggaat cgtcgcgctg aatgcgatct
     2521 tgccgctttc cagaactcgc caaagcaaac ctaccaggct gaaaaagcgc gcgatcgcaa
     2581 actgtgcgcc aacctggaag aggcgattcg tcgctctggt ttacaggacg gcatgacggt
     2641 ttccttccat cacgctttcc gtggcggtga cctgaccgtc aatatggtga tggacgtcat
     2701 cgcgaagatg ggctttaaaa acctgaccct ggcgtccagc tccctgagtg attgccatgc
     2761 gccgctggta gaacacattc gccagggcgt ggttacccgc atttatacct ctggcctacg
     2821 cggtccactg gcggaagaaa tctcccgcgg tctgctggcg gaaccggtgc agatccactc
     2881 tcacggcggt cgtgtgcatc tggtacagag cggcgaactg aatatcgacg tggctttcct
     2941 cggcgtcccg tcctgtgatg aattcggtaa tgccaacggc tacaccggta aagcctgctg
     3001 cggctccctc ggctatgcaa tggttgatgc cgacaacgca aaacaggttg tgatgcttac
     3061 cgaagaactg ctgccttatc cgcataatcc ggcaagcatt gagcaagatc aggttgattt
     3121 aatcgtcaaa gttgatcgcg ttggcgatgc tgcaaaaatc ggcgctggtg ctacccgtat
     3181 gaccactaac ccgcgcgaac tgcttattgc ccgtagcgct gcggatgtga ttgtcaactc
     3241 aggctacttc aaagaaggtt tctccatgca aaccggcacc ggcggcgcat cgctggcggt
     3301 aacccgtttc ctggaagaca aaatgcgtag ccgcgatatt cgcgccgact tcgccctcgg
     3361 tggtattacc gcgacgatgg ttgatctgca cgaaaaaggt ctgatccgca aactgctgga
     3421 tgtgcagagc tttgacagcc atgctgcgca atcgctggcc cgtaacccca atcacatcga
     3481 aatcagcgcc aaccagtacg ctaactgggg ttcgaaaggc gcatcggttg atcgtctcga
     3541 cgtggtggta ctgagcgcgc tggaaattga cacccagttc aacgttaacg tgctgaccgg
     3601 ctctgacggc gtactgcgtg gtgcttccgg tggtcactgc gataccgcga ttgcctctgc
     3661 gctttccatc atcgtcgcgc cgctggtacg cggtcgtatt ccgactctgg tggataacgt
     3721 gctgacctgc atcaccccgg gctccagtgt cgatattctg gtcacagacc acggtatcgc
     3781 agttaacccg gcacgtccgg aactggcaga acgtctgcag gaagcgggca ttaaagtggt
     3841 ttccattgag tggctgcgcg aacgtgcgcg tctgctgacc ggtgaaccac agccgattga
     3901 attcaccgac cgcgtcgttg ccgttgtgcg ttaccgcgat ggctcggtga tcgatgttgt
     3961 gcatcaggtg aaggaataag ccatgcacct gcttcctgaa ctcgccagcc accatgcggt
     4021 gtcaattccc gagctgctcg tcagccggga tgaaaggcaa gcacggcaac acgtctggct
     4081 caagcgccat cctgttccac tggtctcctt taccgtggtt gcgcctgggc cgattaaaga
     4141 cagcgaggtc acacgccgaa tttttaatca tggcgtgaca gccttgcgtg ccttagccgc
     4201 aaaacagggc tggcaaattc aggagcaggc tgcactggtt tccgccagcg ggccggaggg
     4261 catgttgagc attgccgccc cggctcgcga cctcaagctc gccaccattg agcttgaaca
     4321 tagtcatcct ctcgggcggt tatgggatat cgatgtcctg acgcccgaag gcgaaattct
     4381 ctcccgccgc gactattcac tgccgcctcg ccgctgcctg ttgtgcgagc aaagcgcagc
     4441 cgtctgcgcg cgtggaaaaa cccatcaact gaccgattta ctcaaccgca tggaggcact
     4501 gctgaacgat gtcgatgcct gcaacgtcaa ctaaaaccac aaagcttgcg acgtcattaa
     4561 tcgatgagta cgccctgctg ggctggcgcg ccatgctgac tgaagtcaat ctgtcaccga
     4621 aaccaggcct cgtggatcgc attaactgcg gtgcgcacaa agatatggcg ctggaagatt
     4681 tccaccgcag cgcgctggcg attcagggct ggttaccccg tttcattgaa tttggtgcct
     4741 gtagtgcaga aatggcaccg gaagcggtac tccacggatt acgcccaatt ggtatggctt
     4801 gcgaaggtga tatgttccgc gccactgcgg gcgtaaacac gcataaaggc agcatttttt
     4861 ctttagggct gctatgtgcg gcaattggcc gtttgcttca gctcaaccag ccggtaacgc
     4921 caacaaccgt ttgttctacg gcggcaagtt tctgccgtgg cctgaccgat cgcgaactgc
     4981 gtaccaataa ttcacaactg acggcaggtc aacggttgta ccagcagctt ggccttaccg
     5041 gcgcacgcgg tgaagccgaa gcgggttatc cactggtgat caatcacgcc ttgccgcatt
     5101 acctcactct gctggatcag gggttagatc ctgaactggc attgctcgat accttgctcc
     5161 tactgatggc gatcaacggc gataccaacg ttgcatcgcg cggtggcgag gggggcctgc
     5221 gctggctaca gcgcgaggcg caaacattat tgcaaaaagg gggcattcga acccccgccg
     5281 atctcgatta tctccggcag ttcgacaggg agtgtatcga acgaaatctc agtccaggcg
     5341 gcagtgccga cctgctgatc cttacctggt ttttagcaca gatttaatta tttaagcact
     5401 tgataaattt ggaaatatta attttcggag aacccgtatg tctttagcaa aagataatat
     5461 atggaaacta ttggccccac tggtggtgat gggtgtcatg tttcttatcc ctgtccccga
     5521 cggtatgcca ccacaggcat ggcattactt cgctgtgttt gtggcaatga ttgtcggcat
     5581 gatcctcgag ccaattccgg caacagcgat cagttttatt gcggttacta tttgcgttat
     5641 tggcagtaat tacctgctct ttgatgccaa agaattagct gacccagcgt ttaatgcgca
     5701 aaaacaggcg ctgaaatggg gtctggctgg tttttccagc accacggtat ggctggtatt
     5761 tggcgcattt atttttgcat tagggtatga agtttccggg ttaggtcgtc gcattgccct
     5821 tttcctggtg aaattcatgg gaaaacgcac gctgacgttg ggttatgcga ttgtcattat
     5881 cgacattctg ctggcaccgt ttacaccgtc caacaccgcg cgtaccgggg gtacggtttt
     5941 tccggtcatt aaaaacctgc cgccgctgtt taaatcattc cccaacgatc cgtccgcgcg
     6001 tcgtattggc ggctatttga tgtggatgat ggtcattagt accagtctga gttcgtccat
     6061 gtttgtcacc ggtgcggcac caaacgtgct gggtctggag ttcgtcagca aaattgccgg
     6121 tatccagatt agctggttgc agtggttcct ctgcttcctg ccggttgggg ttatcttgct
     6181 tatcattgcg ccgtggcttt cctacgtgct gtacaaaccg gaaatcacac acagtgaaga
     6241 agtggcaacc tgggcgggtg atgaactaaa aaccatgggt gcgctgacac gcagagagtg
     6301 gacgctgatt ggccttgtat tgctcagctt aggtttgtgg gtatttggca gtgaagtcat
     6361 taatgctact gcggttggtc tgctggcagt ttcgctaatg ctggccctgc acgttgtgcc
     6421 ttggaaagat attacccgct ataacagcgc atggaacacg ctggtcaacc tggcaactct
     6481 ggttgtgatg gctaacggcc tgactcgttc tggctttatt gactggttcg ccggtaccat
     6541 gagtacgcac ctggaaggat tctcaccaaa cgcaacggtg attgtactgg ttctggtgtt
     6601 ctactttgca cactacctgt ttgccagcct gtctgcgcac accgcaacca tgctgccggt
     6661 tattctggcc gtcggtaaag gtattccggg cgtaccaatg gaacaactgt gtatcctgct
     6721 ggtgctgtct atcggtatca tgggctgtct gacgccgtat gcaaccggtc ctggggtgat
     6781 tatttacggc tgtggctatg tgaaatcaaa agattactgg cgtcttggcg caatcttcgg
     6841 ggtgatttac atctctatgt tgctgttggt tggctggccg attctcgcca tgtggaacta
     6901 atacgttcgc caaacgctga ataaatcatc acgcccgcca ggtaagccca cctggcgggc
     6961 ttttttatga tttaatagat agtattcagt gtatgtatga gttccacacc cattatgaaa
     7021 gcattctggc gtaacgccgc gttgctcgcg gtttctctgc ttcccttctc ttctgccaac
     7081 gccttagcgt tgcaggcaaa acagtatggc gattttgatc gctatgtcct ggccctctcc
     7141 tggcaaaccg gattttgcca gagtcaacac gatcgaaatc gtaacgaacg agatgaatgt
     7201 cgcctgcaaa ccgaaacgac caacaaagct gattttctga ccgtacatgg tctgtggcca
     7261 ggattgccta aatcggttgc tgcccgtggt gttgatgaac gccgctggat gcgcttcggt
     7321 tgcgctactc gcccaatccc gaatctacca gaagcgcacg ccagccggat gtgttcatcg
     7381 ccggaaaccg gattatcact ggaaacggcc gctaaactaa gtgaagtcat gccaggagct
     7441 ggcggacgtt cctgcctgga acgctacgaa tatgccaaac acggtgcctg ctttggtttt
     7501 gatccggacg catacttcgg tacgatggta cgcctgaatc aagaaattaa agagagcgaa
     7561 gccggaaaat tccttgcgga taattacggt aaaacagtga gccgccgtga ctttgacgcc
     7621 gcctttgcca aaagctgggg aaaagagaac gtgaaagcag ttaagctaac gtgccagggt
     7681 aaccctgcgt atttgactga aattcagatc tcgatcaaag ctgacgccat caacgctccg
     7741 ctttctgcaa actcattctt gccacaacca cacccaggca actgtggcaa aacctttgtg
     7801 attgataaag tgggttatta atcatctgat ttacaaaaca cgctaattcg tcttccataa
     7861 accgtgacga ttaagaatta tacataatgc gctgcattga ggaattccct gaatgtgacg
     7921 caaatcactt caaagagtga gcaacagtca gtatcatcat gtgagttaaa ccctcgccgc
     7981 ctgacggtga gggttttctt ttgggtatgt ttatctgcga cactcgcagt accgacgaca
     8041 tggagtaaaa atgtccagac caactatcat cattaacgac ctggatgccg aacgcatcga
     8101 tattctgctg gagcaacccg cctatgctgg tttgccaatc gccgacgcgt taaacgcaga
     8161 gttggatcgc gcccaaatgt gttcgccaga agagatgcca cacgacgtgg tgacaatgaa
     8221 cagccgggtt aaattccgca atcttagcga tggcgaagtg cgtgtgcgca cgctggtgta
     8281 tccggcaaaa atgaccgata gcaatactca gctttccgtt atggctccgg taggtgccgc
     8341 actgctgggg ctgcgcgttg gcgattccat tcactgggaa cttccgggcg gcgttgcaac
     8401 ccaccttgaa gtgctggaac tcgaatacca gccagaagct gctggcgact acctgcttta
     8461 atcccatctg cacaagcgat aactcgcttt gcccagagga tgcactcgca tcctctttac
     8521 gcaaaatcag caatttttcc tggttctcac tgttcacttt tgctgcaaaa ttattccgtt
     8581 tcgtttgtgg gaataaccat ggcagaagca ttttacatcc tgataggatt tctgattatg
     8641 gcggcgatta tcgtcatggc tgtcctttac ctcgaaaacc actcctgacg atcatattgt
     8701 tccccccggc atcgcattca ccagacctga aaccgccttc tgcgccgcct ctgcgctttg
     8761 tgcaccgggt accagaatca ccttacggca ctcctcttca cgtttttcga aaatctcata
     8821 tccccgggcg gcctcttcaa acggcatata gtgggtaaca atttcttctg gtttcagtaa
     8881 tcctttctca attaacggta ataattctcc cagccatgcg tgaacgtggg tctgtcccat
     8941 tttaaacgtc aaccctttat caaaggcgtc gccaaacagg aaaccgtgaa taaatccagc
     9001 gtagacgccc ggtacgctaa caatgccgcc acgcctgacc gccgcaatac actggcgcaa
     9061 cgctttaccg ctgctgccct ccagtttcag gttagtcagc accgtttccg tggtgctgcc
     9121 tttcgcttca aaaccgacgg cgtctattac tgcatccacg ccccggtgac ctgccgtttg
     9181 ttcaataatt gactgtgccg gatcgctatc ttcatcgaaa ttaatcggga tcgcgccgta
     9241 gcggtcagcg gcgaaacgca agcggtaggg atgatgatca acaacaaaaa tctgttccgc
     9301 accgagcaac cgtgcacagg cgattgtcaa caatcccact ggaccggcac catagactgc
     9361 aacgcttgaa ccttgttgga tctgcgcatt ttttgctgcc tgccatgccg ttggcagaat
     9421 atcagaaagg aaaagggctt tatcatctga aagcaaaggc ggtactttaa acggccccac
     9481 attcccttta gggacgcgga catattccgc ctgtccacca ggaacgccac catacaggtg
     9541 actataacca aacaatgccg cgggagctgg tatctgtttt ttattgagcg cagcgccttt
     9601 acccgcattg gtattttcgc aggcggcata ttgctgcatt cgacagaaaa aacagtcgcc
     9661 acaagcaatg acaaacggaa ttaccacccg gtcgcctttt tgcaaatttt ttacgtcctt
     9721 tccggtttca acgacttccc ccataaattc atgaccaaaa atatcgccat gtttaacctg
     9781 gggtattttg cctcgataaa gatggaggtc agagccacag atcgccgttg ccgtaatacg
     9841 cagaataata tcatctgcct gttcaatgcc cggatcggga acattttcta cctgaacgtg
     9901 atgtgggccg tgataagtca atgctttcat gcaacctcca gagacagttc aaaaaagagt
     9961 cagtctttaa gggtagctat cacagcggga aaacgggcgg taccgcaggt tttgggagaa
    10021 ttcctggtgt tcagtgagaa tatattaatc acagaaaccg ttcgtaagga aaaagaacca
    10081 gacaaatatc agggataaat attataatca tggcgaatga tttgttcatg attaacaggg
    10141 agaaagggtt atgtataaga caatcattat gccagttgat gtatttgaaa tggaattgag
    10201 cgacaaagct gttcgccacg ctgaattcct cgcccaggat gacggagtta ttcatctact
    10261 tcacgtacta cccgggtcag ccagcctgag cctgcaccgt tttgccgctg atgtgcgtcg
    10321 ttttgaagag catctgcaac atgaagcaga agaacgtctg caaacgatgg tcagccactt
    10381 caccatcgat ccttcccgca ttaaacaaca tgtccgtttt ggtagcgtgc gggatgaagt
    10441 caatgagttg gcagaagaac tgggggctga tgttgtagtt attggttctc gcaacccatc
    10501 gatttcgacc catctgttag gttctaacgc ctcgagcgtg atccgccatg ccaatctgcc
    10561 ggtgctggtt gtgcgttaat tgccagaata aatatcccgc cctgcccgga gccatcccgg
    10621 cagggctttt taatgccata aaaaagccgc caggtttgac cccggcggct aagcaattgc
    10681 aggtgaatct tacttcttct tatgcagttt tggtgcgaat caggtagtca aaagcactca
    10741 gagaggcttt ggcaccttcg ccagtggcga tgataatctg cttgtacgga accgtcgtac
    10801 agtcacccgc tgcgaacacg cctttgacgt tggtttcgca tttcgcatca atgataatct
    10861 cacccatgcg gttacgttcg actgcgcctt ccagccagtt ggtgttcggc agcagaccaa
    10921 tctggacgaa aataccggcc agttcgatgt tgtgaatatc gccgctgaca cgatctcgat
    10981 attccagacc aacgactttg ctgccgtcgc ctttcacttc cgtggtttgc gcattcagaa
    11041 taatgtcgac gtttttcagg ctgcgcagtt tgtcctgcag aacctggtcg gctttcattt
    11101 ctggcgcaaa ttcgagcagc gttacgtgct caacgatacc cgccaggtcg attgccgctt
    11161 ccacgccgga gttaccgccg ccgataaccg ctacgcgttt gcctttaaac agcgggccgt
    11221 cgcagtgcgg gcagtaggtc acgcctttgg tgcgatactg atcttcgccc ggaacgttca
    11281 tgttgcgcca ttttgcaccg gtcgccacga taatgctgcg tgctttcagt accgcgccag
    11341 aagctgtttc aatctggtgt agaccacctt caaccgctgc cgggatcagt ttgctggcgc
    11401 tctggctgtc gatcacatca acgtcgtatt catcaacgtg aactttcagt gcgcctgcca
    11461 gtttctgccc ttcagtcttc ggtacagaaa tgtagttttc gatatcaacg gtatcgagga
    11521 tctgaccacc aaaacgttcg cccatcagac cggtacggat gcctttacgt gcggagtaaa
    11581 ttgctgccgc tgcacccgcc gggccggaac cgacgattaa tacgtcataa gcatcacgct
    11641 tgttcagctc ttctgccgca cgtttttccg cgccagtatc aattttggca acgatttcag
    11701 tcaacgtcat gcggccctga ccaaactctt tcccgtttac gaacactgcc ggaacgccca
    11761 tcacgttgcg atcggtgatt tcgttctgga aggtgccgcc gtcaattgca gtgtgcttga
    11821 tgcgcgggtt caatacgctc atcaggttca gcgcctgcac cacgtccggg cagttatggc
    11881 aagagagcga gtaataggtt tcgaattcaa aatcaccgtc aatatggcga atctgctcca
    11941 gcagagactg cgcttctttc gacggatgac caccggtcca cagtaacgcc agtaccagcg
    12001 aggtgaactc gtggcccagc ggggagcctg caaaacgtgg cccctggttg gaacctgggt
    12061 tggtgatcag gaaagacggc ttacgcaccg gcaagctgtt atcttcttta aaggtgactt
    12121 tgtctgacag ttctgcgatt tcagccaaca gttccttgat ttctgccgat ttagcgctgt
    12181 catccagcgt ggcaattaac tcaacaggct tggtcaattt ctcaaggtaa gccttgagtt
    12241 gagttttcat atttgtgtcg agcatgttta tctcctgggc tttaaacatc atcatgcaag
    12301 cggcctctgc ggctacctgg atgcagcttg catcatggtg cggggaagaa aatcgggtgc
    12361 cgcagcaccc gaagaattag gtgaatttcc gaagtatttc gcgccggagt tggtagcgcg
    12421 aaagacgaag gaaatttaga ttttaccaac caggtccaga gacggagcca gagttgcttc
    12481 accttctttc catttagccg ggcaaacttc acctgggtga gaagctacgt actgtgctgc
    12541 tttgatttta cgcagcaggt cagacgcgtc acggccaatg ccttcagcgg taacttcgat
    12601 tgcctggatg ataccctgcg ggtcaacaac gaaggtcgca cgatcagcca gaccttcatc
    12661 ttcacgcatg ttgtcgaagt tacgggtcag ggcgccagtc gggtcgccga tcatcgcata
    12721 tttgatttta gcgatggttt cagagctgct gtgccatgct ttgtgggtga agtgagtatc
    12781 ggtagatact gcgtatacgt ctacgcccag tttctgcagt tcttcgtagt ggtcagcaac
    12841 gtcacccagt tcggtcgggc atacgaaagt aaagtcagcc gggtagaaga agaagacgct
    12901 ccagcggcct tctgtatctt tttcggtgat ttcgatgaat tcgccgtttt tgaatgcctg
    12961 gtttttaaaa ggtttaattt tggtgttaat caaggacatc tatacttcct ccgtgttttc
    13021 gatgagatgt aaggtaacca atttttgccg attcggctaa tgcgtttcca ttatcaaatc
    13081 gataagtttt accttacaac cttcgtaaga caactttgtg aactttgcac cgccagcggc
    13141 gataaaaagt aaaatggccc cctttccagg tgaccgtgta atctgaacta ccctttggta
    13201 tcttcagcga ctgcctgcag gctgagtgtt gcccttacaa atgcaacaac gacatggatt
    13261 acaacaccct cataaacaaa gggcaatcac ctgatctaag ctcttaccta tgacagtgat
    13321 aggttatgcc ttttactcga cttttgcact gactgaaaag gacaaattaa tgttaaaaaa
    13381 gatactttta ctggctctgc ttcctgcaat cgccttcgca gaggaacttc ctgctccagt
    13441 aaaagcgatt gaaaaacagg gcattacaat catcaaaacg ttcgatgccc ccggaggaat
    13501 gaaaggttat ctcggaaagt atcaggatat gggcgtcacc atctacctga ctccagatgg
    13561 taagcacgct atctctggtt acatgtacaa cgagaaaggt gaaaacctga gtaacacact
    13621 tatcgaaaaa gaaatttacg caccagccgg acgcgaaatg tggcaacgga tggaacaatc
    13681 ccactggctc ctcgacggta aaaaagatgc gccggtcatt gtctacgtct tcgccgatcc
    13741 gttctgccca tattgtaaac agttctggca gcaggcgcgc ccgtgggtag attctggcaa
    13801 agtgcaatta agaacattgt tggttggggt tatcaagcca gaaagcccgg cgacagcagc
    13861 ggcaattctt gcctccaaag atcccgcaaa aacctggcaa caatatgaag cctctggtgg
    13921 caagcttaag ctaaacgtgc ctgcaaacgt aagtacagag caaatgaaag tgttaagtga
    13981 caatgagaaa ctgatggacg atctgggggc aaatgtcacg ccggctatct attacatgag
    14041 taaggaaaat acgctacaac aggccgtggg gttgcccgat cagaaaacgc ttaatatcat
    14101 tatggggaat aaataaaaga taattcatgg atggttgaaa tacatctctg cttttcaacc
    14161 atcctaaaaa agcatga
//
LOCUS       DBANUU010000113        14145 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000113 DBANUU010000000
VERSION     DBANUU010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.113"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            109..651
                     /gene="ecpR"
                     /locus_tag="ACIE68_003477"
                     /gene_synonym="matA"
     CDS             109..651
                     /gene="ecpR"
                     /locus_tag="ACIE68_003477"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFX5909739.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLSTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            726..1313
                     /gene="ecpA"
                     /locus_tag="ACIE68_003478"
                     /gene_synonym="matB"
     CDS             726..1313
                     /gene="ecpA"
                     /locus_tag="ACIE68_003478"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFX5909740.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFTII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1371..2039
                     /gene="ecpB"
                     /locus_tag="ACIE68_003479"
                     /gene_synonym="yagY"
     CDS             1371..2039
                     /gene="ecpB"
                     /locus_tag="ACIE68_003479"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFX5909741.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2065..4590
                     /gene="ecpC"
                     /locus_tag="ACIE68_003480"
                     /gene_synonym="yagX"
     CDS             2065..4590
                     /gene="ecpC"
                     /locus_tag="ACIE68_003480"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFX5909742.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4580..6223
                     /gene="ecpD"
                     /locus_tag="ACIE68_003481"
     CDS             4580..6223
                     /gene="ecpD"
                     /locus_tag="ACIE68_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFX5909743.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6192..6902
                     /gene="ecpE"
                     /locus_tag="ACIE68_003482"
     CDS             6192..6902
                     /gene="ecpE"
                     /locus_tag="ACIE68_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFX5909744.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7215..7544
                     /gene="ykgJ"
                     /locus_tag="ACIE68_003483"
     CDS             7215..7544
                     /gene="ykgJ"
                     /locus_tag="ACIE68_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFX5909745.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7792..8406)
                     /gene="yagU"
                     /locus_tag="ACIE68_003484"
     CDS             complement(7792..8406)
                     /gene="yagU"
                     /locus_tag="ACIE68_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFX5909746.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            8824..9513
                     /gene="paoA"
                     /locus_tag="ACIE68_003485"
     CDS             8824..9513
                     /gene="paoA"
                     /locus_tag="ACIE68_003485"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFX5909747.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVLAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9510..10466
                     /gene="paoB"
                     /locus_tag="ACIE68_003486"
     CDS             9510..10466
                     /gene="paoB"
                     /locus_tag="ACIE68_003486"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFX5909748.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFAQVSVAAIIHPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDTLFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            10463..12661
                     /gene="paoC"
                     /locus_tag="ACIE68_003487"
     CDS             10463..12661
                     /gene="paoC"
                     /locus_tag="ACIE68_003487"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFX5909749.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEGTPDK
                     NVGDFDGAFSSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPTIP
                     NNTTHRPAPLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEPNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACVKLREMIASAVGFDPEQSQFADGKITNGTRSAMLHEATAGGRL
                     IAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            12671..13627
                     /gene="paoD"
                     /locus_tag="ACIE68_003488"
     CDS             12671..13627
                     /gene="paoD"
                     /locus_tag="ACIE68_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFX5909750.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            13678..14016
                     /locus_tag="ACIE68_003489"
                     /pseudo
     CDS             13678..14016
                     /locus_tag="ACIE68_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
BASE COUNT         3500 a         3772 c         3788 g         3085 t
ORIGIN      
        1 ttacttccgg tttactaagg aaactgaatg cacctgtaaa aattacaggt ttggaaagta
       61 gtgacatggc aaagtgatta cagtagggac tatgaggtta aaaaccatat ggaatgtcaa
      121 aaccgttctg ataaatacat ctggtctccc catgacgcct acttctataa aggactatct
      181 gaactgattg tggatatcga cagattaatt tatctatcgt tggagaaaat tagaaaagat
      241 ttcgtgttta tcaatctcag tacggattct ttatctgaat ttataaaccg tgataatgaa
      301 tggttatccg cggtaaaggg gaaacaggtc gtattgattg cggccagaaa gtcagaagcc
      361 ttagcaaatt attggtatta caatagcaat attaggggcg tggtatacgc tggactgagt
      421 cgtgatatta gaaaagaact ggcctatgtg attaatggca ggttcctgag aaaagatatt
      481 aagaaagata aaatcacgga ccgggaaatg gaaattatcc gcatgacggc ccagggaatg
      541 caacctaaat cgattgccag aattgaaaat tgtagtgtga agacagtgta tacccatcgg
      601 cgtaatgctg aggccaagct gtactcaaaa atatataagt tggttcagta aactccaggc
      661 aagttagttt taaaaaatga ctcactggga catcacgtcc tcaattcaac tcgggaagaa
      721 atgcaatgaa aaaaaaggtt ctggcaatag ctctggtaac ggtgtttacc ggcatgggtg
      781 tggcgcaggc tgctgacgta acagctcagg ctgtagcgac ctggtcggca acagccaaaa
      841 aagacaccac cagtaagctg gttgtgacgc cactcggtag cctggcgttc cagtatgccg
      901 aaggcattaa aggttttaac tcacagaaag gtctatttga cgtggctatc gagggtgact
      961 caacggctac cgcctttaaa ctgacctcac gtcttatcac caacacatta acccagttgg
     1021 atacctcagg ttccacactg aatgtgggcg tggattataa cggcgcggca gtcgaaaaaa
     1081 ctggcgatac cgtgatgatc gataccgcca acggcgtact gggcggcaac cttagcccgc
     1141 tggctaacgg ttacaatgcc agcaatcgta ccaccgcaca ggatggtttc accttcacca
     1201 tcatcagcgg caccaccaat ggtaccaccg cagtaaccga ttacagcact ctaccggaag
     1261 gcatctggag cggcgacgtt agcgtacagt tcgacgcgac ctggaccagt taatctctct
     1321 gatgtaccag caggggtagc ccccctgctt tattcctgga cggaattgtt atgaaaaagc
     1381 accttctgcc tctcgctctg ctgttttccg gaatatctcc ggcccaggcg ctggatgtcg
     1441 gcgatatatc atcgtttatg aacagtgaca gcagcacgct gagcaaaacg atcaaaaaca
     1501 gtaccgacag tggtcgcctt atcaatatcc gtctcgaacg gctctcttca ccgcttgacg
     1561 acgggcaggt tatctcaatg gacaagccgg atgagttgct actcactccc gccagcttgc
     1621 tgctacccgc ccaagccagc gaagtgatcc gcttcttcta taagggaccc gcagatgaaa
     1681 aagagcgcta ctaccgcatt gtctggtttg atcaggccct cagtgatgca cagcgcgata
     1741 atgccaaccg cagcgctgtg gccactgctt ccgcccgcat cggcaccatt ctggtcgtcg
     1801 caccccgcca ggcaaactac cactttcagt acgccaacgg ctccctgaca aatacaggaa
     1861 atgcgacgct gcggatcctc gcctacggcc cctgcctgaa agccgccaat ggtaaagagt
     1921 gtaaagagaa ttactaccta atgccgggca agtcgcgtcg ttttacccgc gtggacacgg
     1981 cggataacaa aggacgggtt gcactttggc agggtgataa gttcattccc gtgaaataga
     2041 tagctgtgca gatggataac gacaatgcct ttacgacggt tctccccagg actgaaagcc
     2101 cagtttgcct tcggcatggt ctttttgttc gttcagcccg atgccagcgc tgctgacata
     2161 agtgcgcagc aaataggtgg ggtgattatt ccgcaggcct tcagtcaggc gcttcaggac
     2221 ggcatgagcg tcccgctcta tattcatctc gccggtagcc agggtcgcca ggacgatcag
     2281 cgaatcggca gcgcttttat ctggctggac gatggacagc tacgcatccg gaaaatacag
     2341 ctggaagaga gtgaagataa cgccagtgtc agcgaacaaa ctcgacagca gctgatggct
     2401 ctggcgaacg ccccgttcaa tgaggccctt accatccccc tgactgacaa cgcgcagctg
     2461 gatctcagct tgcgccaact gctgctgcag ctggtggtca agcgcgaagc gctgggcacc
     2521 gtactacgct cacgtagcga agacatcggg cagtccagtg ttaacaccct cagcagtaat
     2581 ctgagctata acttgggcgt ctataacaac cagttgcgta acggcgggag caacacatcc
     2641 agctatctgt cgctgaataa cgttactgcg ctgcgcgaac atcatgtggt gctcgacggc
     2701 tcgctgtacg ggatcggtag cggtcaacag gacagtgaat tatataaagc gatgtatgaa
     2761 cgcgattttg ccggtcaccg atttgccggt ggaatgctcg acacctggaa cttgcagtcc
     2821 ttagggccga tgaccgccat ttcagcaggg aagatttacg gcctttcctg gggaaaccag
     2881 gccagctcca ccatcttcga cagcagccag tcagccacgc cagtgatcgc ctttttaccg
     2941 gcggcgggcg aagtacatct cacccgtgat gggcggctac taagcgttca gaacttcact
     3001 atgggcaatc atgaagtgga tacccggggt ctaccgtacg ggatttacga tgtggaagtt
     3061 gaggtgatcg ttaacggtcg cgtgatcagc aaacgcaccc agcgggtcaa taagctgttt
     3121 agccgggggc gcggcgtcgg tgcaccactg gcgtggcagg tatggggcgg tagctttcat
     3181 atggatcgct ggtcggaaaa cgggaaaaag acgcgaccag ctaaagagag ttggctagca
     3241 ggtgcctcga cctccggctc attgagtacg cttagctggg cggcaacggg atatggatac
     3301 gataatcagg cggtgggtga aacccgtctg acgctgccgc ttgggggggc gatcaacgtt
     3361 aacctgcaaa atatgctggc cagtgacagc tcatggagca gcatcggcag catcagcgcc
     3421 actctaccgg gaggctttag ttcgctgtgg gttaatcagg aaaaaacccg cattggcaat
     3481 caattgcgac gtagcgatgc cgataaccgt gcaatcggcg gcacactcaa cctgaactca
     3541 ctgtggtcga agctgggcac attcagcatc agctacaatg atgaccgccg ttacaacagc
     3601 cattattaca cggcagatta ctatcaaaat gtctacagcg gtacctttgg ttcgcttggc
     3661 ctgcgggccg gtattcagcg ctataacaac ggcgacagca acgccaatac agggaaatat
     3721 atcgctctcg atctctcgct accactgggc aactggttta gcgcagggat gacccatcaa
     3781 aacggctaca ccatggcaaa cctgtcagca cgcaaacagt ttgatgaagg aaccattcgc
     3841 actgttggtg ccaatctgtc acgtgccatc tccggcgata ccggtgatga caaaaccctc
     3901 agcggtgggg cgtatgcaca gttcgacgct cgttacgcca gcggaacgct gaacgtcaat
     3961 agcgcggcgg acggctacat caataccaac ttgaccgcca atggcagcgt cggctggcag
     4021 ggtaaaaaca tcgctgccag cgggcggact gatggcaacg ctggggtgat attcaacacc
     4081 gggctggagg acgacggtca gatcagcgcc aaaatcaacg ggcggatttt cccgcttaac
     4141 ggcaagcgta actatctccc gctctctccc tatggaagat atgaggtgga gttacagaac
     4201 agcaaaaact cactcgacag ctacgatatc gtcagcggcc gcaaaagcca tctgactctc
     4261 tatccaggca atgtcgctgt cattgagcca gaggtgaagc agatggttac cgtctccggt
     4321 cgtatccgtg cggaagacgg cacactgctg gctaacgcac ggattaacaa ccatatcggc
     4381 cgaacccgaa ccgatgaaaa cggcgagttt gtcatggacg tggataagaa atatcccact
     4441 atcgattttc gctacagtgg caataaaacc tgcgaagtgg cactggaact caaccaggcg
     4501 cgcggtgccg tctgggtcgg tgatgtggtc tgcagcggcc tctcatcgtg ggcggcggtg
     4561 acgcagacag gagaagagaa tgagagttaa cctactaata acgatgataa ttttcgcgct
     4621 aatctggcca gtaactgagc tcagagcggc agtgagcaaa acaacctggg cggatgcacc
     4681 ggcacgcgag tttgtgtttg tcgaaaacaa ctcagacgac aactttttcg tcactcctgg
     4741 cggggcgctg gatccgcgcc tgaccggtgc caaccgctgg accggtttaa aatacactgg
     4801 ttcaggaacc atctatcagc aaagcctcgg ctacattgat aacggttaca acaccggcct
     4861 ttataccaac tggaagtttg atatgtggct ggaaaattca ccagtttcat ctcctttaac
     4921 tggcttgcgc tgcatcaact ggtacgctgg gtgtaatatg accaccagtc ttatcctgcc
     4981 gcaaaccacc gacgccagtg gattttatgg cgcgacggtg accagcggcg gcgcgaagtg
     5041 gatgcacggc atgttgtcag acgcgtttta ccagtatctg caacaaatgc ccgtcggcag
     5101 cagctttaca atgaccatca atgcctgcca gacctctgtg aactatgacg ccagcagcgg
     5161 cgcacgctgt aaggatcagg cctccggcaa ctggtatgtt cgcaacgtca cccatacgaa
     5221 agcagcaaat ctgcggttaa taaataccca ctcgctggcg gaggtattta tcaacagcga
     5281 cggagtaccg actctgggcg aagggaacgc cgactgccgg acacagacca tcggcagcct
     5341 ttcaggatta agttgtaaga tggtcaacta taccctgcaa acaaacggac tcagcaacac
     5401 ctcaatccat atattcccgg cgatcgccaa ctcgtcgtta gcctcggccg tcggggcgta
     5461 cgatatgcag ttcagtctga atggcagttc atggaaaccg gtgagcaata ccgcctatta
     5521 ctacaccttc aacgagatga agagcgcaga ctcgatctat gttttcttct cgagcaactt
     5581 ctttaagcag atggtgaacc tcgggatcag cgatatcaac accaaagatc tattcaactt
     5641 tcgctttcag aacaccacat caccggagtc tggctggtat gaattttcta cctccaacac
     5701 gctgattatc aaaccccgtg atttcagcat cagtattatc tccgatgaat atactcagac
     5761 accgtcgcgg gagggatatg ttggcagcgg cgagtcggca ctcgatttcg gctatatcgt
     5821 aaccaccagc ggtaaaacag ctgccgacga agtgctgatc aaggtgaccg gacccgcgca
     5881 ggtgattggc gggcgctcct attgtgtctt cagctccgat gacggtaagg cgaaagtacc
     5941 gttcccggcg acactttcct ttattacccg caacggagct acaaaaacct acgatgccgg
     6001 gtgcgatgat agctggcggg atatgaccga tgcgctgtgg ttgaccacac cgtggactga
     6061 tatctctggc gaagtggggc agatggataa gaccacagtc aaattttcga ttccaatgga
     6121 taacgccatt tcgctgcgta cggtagatga taacggctgg tttggcgaag tcagcgcttc
     6181 aggagagatt catgttcagg cgacgtggcg taacattaac taaggccctg ctgacagcgg
     6241 tctgtatgct ggcggcacct ttgacacagg cgatttcggt cggcaatctg acattttcgc
     6301 tgccgtccga gactgacttt gtcagcaaac gtgtagtgaa taacaacaaa agcgcacgga
     6361 tataccgtat tgccatcagt gctattgata gcccgggcag cagtgaattg cgcacccgac
     6421 cggtggatgg tgaactgctt ttcgcccccc gccaactggc gttgcaggct ggtgagagcg
     6481 agtattttaa attttactat catggtccac gggataaccg cgagcgctac taccgggtct
     6541 catttcgcga agtccccact cgtaaccaga caagacgtag cccaaccggc ggcgaggtca
     6601 gcacggagcc ggtggtggtg atggatacca ttctggtagt acgaccacgt caggttcagt
     6661 ttaaatggtc cttcgacaag gtgacaggaa cggtaagtaa cactggcaac acatggttta
     6721 agctactgat taagccagga tgtgattcga ccgaagagga aggcgatgcc tggtatctac
     6781 gtccgggaga cgtggttcat cagcctgagt tacgtcagcc ggggaatcat tatctggtct
     6841 ataacgacaa attcattaag attagcgatt cctgtccggc taagccccct tcggcggact
     6901 aagacttatc catcggtcga gggaagaatt ccacctcaga gctccaaaaa tcgtttaaat
     6961 gatgtggaag cgatcgtcaa tgtcacctgt aacgatgggc tttctcatat gcacaccata
     7021 gtgttcagga gacacggaaa ggtatcaggc agcctgctaa cgagtggttt aatcacgaat
     7081 tagtacgtaa aatcggtaac ggctggaaat cattcaatac tcacactatc gaaagttccc
     7141 cagccaaccg cggtacgttc ttacatacga tgtaccgctg ttctctttac gatttttagc
     7201 tgtactggtg aattatgagc aatctgaatc catgcatgac gtgtggtgcc tgttgtgcat
     7261 ttttccgcgt ctctttttac tgggccgaag ccgacgatgc tggcggtact attcccgcca
     7321 ggctcactga acaaatatcc ccttttcacc gatgcatgag cggtaccaat cagaaaaacc
     7381 cccgatgtat tgcccttgca ggaaccccgg gcaaaaatgc ctgctgcacg atatataaaa
     7441 atcgatcgtc cacatgcaga gaattcgcca tgtctggtga gaacggagtc gtcaatgagg
     7501 cttgcaatcg tgcaagggct aaatacgggc tgacaccgtt ataaacatac aataattaat
     7561 tgcactgccc cgccagcgat aatagcgggg cttcgttttt caggggtaac aaaacccgat
     7621 acttcttcta tttgccagca acaatgcctc tcttctgtag cgttcttcgg atctacatca
     7681 tcctgaagta gcgcgatata ctcactgact atctgcatca gaatataaaa agcaatgttt
     7741 ttaacctata aaaatggcgc tgtatttgcg ccatttttat cattcaatgc attatctgtt
     7801 tgagcctaaa gggatctcag ggtctggctc atgagtaatt ctgtttcgta aatctctgcg
     7861 aataatttca atagaccaga accagactaa atgtccaaaa atttcagaaa cattctcata
     7921 ccacgggaga tcaaacagag gtggcgtcag tcccatgaga gggaatgaaa tcatatgaac
     7981 aaaaagttgg gctaaagcac ctgccagtaa gccctgccag agtttaattt ttggaaatac
     8041 ttcagcgacc acacaataac cgacagcaaa cactatcgaa aagataatgt gcgtaacacc
     8101 aacccagtta aagacatgcc cggcaaaggt ataaacagcc gcattgggat ctgtcagccc
     8161 caaccagtct cgaagaaaaa tatacggtgg attgagaaaa ttacgcgagc aatcaatttg
     8221 gcctgcagcc ctgattaatg attccgggcc acacgctgca ttaaacatat ccaccgggct
     8281 acgtggcggc aatggaactt cagcccccca cttcacgaat gcggaaacaa cgccagcaat
     8341 cagcccaatg aatgcagcaa gaccataacg tctgcggttc ggtggagttt gttcaaatat
     8401 attcatatct accctgcttg taccattatg ttatacacct cttcaggagt attcataaaa
     8461 caaggcaaat gtaaagaact gtattgtttt gtataacaag atggtttcct aatcgccaat
     8521 gaatataagc tccatcattt ctccctattt ttatattaaa agtgacagag atttgcaggg
     8581 tgatgcagag ctgaaaatca caggtttcct tattggtttt tgcatcgtac aactaaagca
     8641 ataaaccagc tcccatcatc atcaacatcc cggcgaacat gaatttatta gccgggctca
     8701 tagctgcatt cgcgttttaa gaataatcct cctgctgtcg ccgactatgc ttaacattta
     8761 aaaaagcatc agcactctcg caacgcactc ttattttccc ctttagaata ccggaggcct
     8821 ggtatgagca accaaggcga ataccccgaa gataatcggg ttgggaagca cgagccgcac
     8881 gatttaagtt tgacccgtcg cgatctgatt aaagtgagcg ccgcaacagc ggcgaccgcc
     8941 gtggtttatc ctcattctac gctggcggca agcgttctgg cagctacacc cgcgccagag
     9001 ataatgcccc tgacactgaa ggtgaacggc aaaaccgagc agcttgaggt ggatacccga
     9061 accacgctac tggacgcttt gcgtgaaaat ctgcatttga tcggtaccaa gaaaggttgc
     9121 gatcacggac agtgcggagc ctgtaccgtg ctggtcaatg gtcgcaggct taatgcctgc
     9181 ctgacgcttg cagtcatgca tcagggggcc gagatcacca ccattgaagg cctgggctcg
     9241 ccagataatc ttcaccccat gcaggcggcc tttatcaagc atgatggctt ccagtgcggc
     9301 tactgcacct ccgggcaaat ttgctcatca gtagcggtgc taaaagagat tcaggacggc
     9361 attcccagtc acgtcacggt cgatttggtt tccgctccag aaacaactgc cgatgagatc
     9421 cgtgaacgta tgagcggcaa catctgtcgc tgtggtgcat acgctaacat ccttgccgcc
     9481 attgaagatg ctgcggggga gataaaatca tgaaggcgtt tacctatgaa cgagtgaaca
     9541 ccccagccga ggcggcactt agcgctcagc gcgtacccgg cgcaaaattt atcgcgggcg
     9601 ggaccaatct gctggacctg atgaagctgg aaattgaaac gcccacccac cttatcgatg
     9661 tgaacggcct cgggctcgat aagattgaag tgaccgacgc gggtgggctg cgcatcggcg
     9721 cactggtacg gaacaccgac ctggcggctc acgagcgcgt gcgtcgtgat tacgcggtac
     9781 tctcccgcgc cctgctcgct ggcgcatctg gtcagttacg taatcaggca accaccgcag
     9841 gtaatctgct ccagcgcacg cgctgcccct atttttacga caccaatcag ccctgcaata
     9901 agcgcctgcc cgggagcggc tgcgcggcgc ttgaaggctt tagccgtcag cacgcggtgg
     9961 taggcgtaag cgaagcctgc attgccaccc atccgagcga tatggcggtc gcaatgcggt
    10021 tgctggatgc ggtggtggaa accatcacgc cggagggaaa gactcgcagt atcacactgg
    10081 ctgattttta tcaccctccg ggaaaaacgc cgcacattga aaccgccctg cttcccggtg
    10141 agcttatcgt tgcggtgacg ttacctccgc cgctcggcgg aaaacatatc taccgtaagg
    10201 tgcgcgatcg cgcctcctac gcctttgccc aggtatcggt cgcggcgatt attcaccctg
    10261 acggcagcgg gcgcgtcgcg ctgggcggag tagcacataa gccctggcgc attgaggctg
    10321 cggatgctca gctatcccag ggggcgcagg ccgtatatga cacgctgttc gccagcgccc
    10381 atcccaccgc tgaaaacacc tttaaactcc tgttggcgaa gcgaacgctt gcctccgtac
    10441 tggctgaagc gagggcacag gcatgaaatt tgataaaccc gcaggggaaa acccgatcga
    10501 tcagctgaag gttgtcggtc gtccccatga ccgcatcgac ggaccgctga aaactaccgg
    10561 cacggcacgc tacgcctacg aatggcatga agaggccccc aacgccgcct atggctatat
    10621 cgtcggttcc gccattgcca aaggacgcct caccgccctt gatacggacg ccgcgcaaaa
    10681 agcgccgggc gtactggctg tcattaccgc cagtaacgcc ggggcactcg gcaaaggcga
    10741 caaaaacacc gccaggctgt taggcggccc cactattgag cactatcatc aggccattgc
    10801 gctggtagtg gccgagacct tcgaacaggc gcgagcggcg gcctcgctgg tgcaggcgca
    10861 ctatcgccgt aataaaggag cttactccct ggcggacgaa aaacaggccg tcaatcagcc
    10921 gccggaaggc acgcccgaca aaaacgtcgg tgactttgac ggggctttct cctccgctgc
    10981 ggtgaagatt gatgctacct acacgacccc ggaccagagc catatggcga tggagccgca
    11041 tgcctcgatg gccgtctggg atggaaataa gcttactctc tggacctcaa atcagatgat
    11101 tgactggtgc cgcaccgatc tggcaaaaac gctgaaagtg cccgtggaga atgtgcgtat
    11161 tatctccccg tatatcggcg gagggtttgg cggcaagctg ttcctgagaa gcgatgcgct
    11221 gctggcagcc ctcgccgccc gagcggtgaa acgtccggtt aaagtgatgc tcccccgccc
    11281 cactattccc aataacacca cgcaccgccc cgcacccctt cagcacctgc gtattggtgc
    11341 tgaccagagc gggaaaatca ccgctatctc acatgaaagc tggtccggaa acctgcccgg
    11401 cggcacgccg gaaacggcgg tacagcaaag cgaattactc tacgccgggg caaaccgtca
    11461 taccggcctg cggctcgcca cgcttgattt gccggaaggg aacgccatgc gtgcgcccgg
    11521 cgaagccccc ggtctgatgg cgctcgaaat cgcgatcgac gaactggcgg aaaaagcggg
    11581 catcgatccc gtcgagtttc gcatcctgaa tgacactcag gttgaccccg ccgacccgac
    11641 gcgccgcttc tctcgccgtc agcttatcga gtgcttgcgc accggagcgg ataaatttgg
    11701 ctggaagcag cgcaacgcca cacccggaca ggtgcgcgac ggggagtggc tagtcggcca
    11761 cggcgtcgcg gcgggctttc gcaataatct gctggaaaaa tcgggggctc gggttcacct
    11821 cgaaccaaac ggcaccgtta ccgtggaaac ggacatgacc gacattggca ccggcagcta
    11881 caccattctg gcccagacgg cagcggaaat gcttggcgta ccgctggagc aggttgcggt
    11941 tcacctcggc gattccagtt tcccggtttc tgcgggttct ggtggacaat ggggcgcgaa
    12001 tacctccacc tccggcgttt acgccgcctg tgtgaagctt cgcgaaatga ttgcctcggc
    12061 agtcgggttt gatcctgagc agtcgcagtt tgccgacggc aagattacca acggtacccg
    12121 aagcgccatg ctacatgagg ccaccgcagg cggcagactg atagcggaag agagcattga
    12181 attcggaaca ctgagcaagg agtaccagca gtcgaccttt gccgggcatt ttgtggaggt
    12241 cggcgtgcat agcgcgacgg gagaagttcg ggtccggcgt atgctcgctg tgtgtgctgc
    12301 aggacgcatc ctgaatccga aaactgcacg cagccaggtc attggcgcaa tgactatggg
    12361 catgggcgcg gcactgatgg aggagctggc ggtggatgac cgtttgggct acttcgttaa
    12421 tcacgatatg gcggggtatg aggtgccggt ccatgcggat atcccaaaac aggaggtgat
    12481 tttcctggat gataccgacc ccatatcctc cccgatgaag gccaagggtg tcggtgagct
    12541 gggcctgtgc ggcgtgagcg cggctatcgc caacgcggtg tataacgcca ccggtattcg
    12601 ggtacgcgat tatcccatca ctctggataa gctgctcgat aagctgccgg atgtggttta
    12661 aggagaaaca atgtcatacc cgctttttga taaagacgaa cactggcata agccagagca
    12721 ggcgtttctc accgatgacc accggaccat tctgcgcttc gccgtagagg cgctaatgtc
    12781 cggtaaagga gcggtgctgg tgacgctggt ggagatacgc ggcggcgcgg cgcgcccgct
    12841 cggggcgcag atggtggtgc gcgaagatgg tcgttactgc ggttttgtct ctggcggctg
    12901 cgtggaggcc gctgccgctt ttgaagcgct ggagatgatg ggctcaggcc gcgatcgcga
    12961 aattcgctat ggcgaaggtt cgccgtggtt tgacatcgtt ctgccctgcg gcggtgggat
    13021 cacgctgacg ctccataaac tacgctcggc acagcctctg ctcgccgtgc tgaaccgcct
    13081 ggaacagaga aaaccggcgg ggctgcgcta cgatccgcaa gcacaatcgt tggtgtgcct
    13141 gcccacgcaa acccgaacgg gctggaatct caatggcttt gaggtggggt tcaggccatg
    13201 cgtcaggctg atgatttacg gacgttctct tgaggcgcag gcaaccgcga gtcttgcagc
    13261 agccacaggc tatgacagcc atatcttcga tctttttccg gcctcagcca gcgctcagat
    13321 cgataccgat acggcggtca ttttgctgtg ccatgatctc aaccgggagc tgccagtgtt
    13381 gcaggccgcg cgagaagcaa aaccctttta tctcggcgca ttgggcagct atcgaaccca
    13441 cactttacgt ctgcaaaagc tccacgagct gggatggtcc agggaggaga cagcgcaaat
    13501 ccgggcaccc gtcgggatat ttcccaaagc ccgggatgcg catactctgg cactctccgt
    13561 gctggcagaa gtcgcctctg tacgtctcca tcaggaggag gattcatgcc tgcccccgtc
    13621 gtcctgatcc ttgcggccgg gcgtggagag cgctttctcg cctccggggg aaatacccat
    13681 aagtgtatcg gctggcgtca gtccccggag gttgcgcctt atcgctggcc atttgaagaa
    13741 aacgggagaa ctttcgacct tgcgattgaa ccgcagatta cgactaatga tctgcgtctg
    13801 atgttgcagc tggctcttgt cggtgaagga ataacaattg ccactcagga aactttcagg
    13861 ccatatattg aaagcggtaa gcttgtatcg ctgcttgatg actttcttcc acaatttcca
    13921 ggcttctatc tgtatttccc acagcgtcgc aatattgcac caaagctccg cgccctgatt
    13981 gaccacgtca aggaatggcg gcagcaattg gcttaaatgt ctgcacctgc attgcctgat
    14041 gtcagaacag tattttgatg aattgccagg gttacaatgg cacaaatacg gcacaggagg
    14101 aaacgtggtg tatttaaata tggggtaact tattgattta aatgg
//
LOCUS       DBANUU010000114        14130 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000114 DBANUU010000000
VERSION     DBANUU010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.114"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            286..2991
                     /gene="malT"
                     /locus_tag="ACIE68_003490"
     CDS             286..2991
                     /gene="malT"
                     /locus_tag="ACIE68_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFX5909751.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(3034..4050)
                     /gene="rtcA"
                     /locus_tag="ACIE68_003491"
     CDS             complement(3034..4050)
                     /gene="rtcA"
                     /locus_tag="ACIE68_003491"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFX5909752.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(4054..5279)
                     /gene="rtcB"
                     /locus_tag="ACIE68_003492"
                     /pseudo
     CDS             complement(4054..5279)
                     /gene="rtcB"
                     /locus_tag="ACIE68_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
     gene            5468..7066
                     /gene="rtcR"
                     /locus_tag="ACIE68_003493"
     CDS             5468..7066
                     /gene="rtcR"
                     /locus_tag="ACIE68_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFX5909753.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRTPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(7048..7806)
                     /locus_tag="ACIE68_003494"
     CDS             complement(7048..7806)
                     /locus_tag="ACIE68_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFX5909754.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(7823..8653)
                     /gene="glpG"
                     /locus_tag="ACIE68_003495"
     CDS             complement(7823..8653)
                     /gene="glpG"
                     /locus_tag="ACIE68_003495"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFX5909755.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(8698..9024)
                     /gene="glpE"
                     /locus_tag="ACIE68_003496"
     CDS             complement(8698..9024)
                     /gene="glpE"
                     /locus_tag="ACIE68_003496"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFX5909756.1"
                     /translation="MDQFECINVAVAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            9214..10719
                     /gene="glpD"
                     /locus_tag="ACIE68_003497"
     CDS             9214..10719
                     /gene="glpD"
                     /locus_tag="ACIE68_003497"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFX5909757.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(10773..11378)
                     /locus_tag="ACIE68_003498"
     CDS             complement(10773..11378)
                     /locus_tag="ACIE68_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909758.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(11381..12148)
                     /locus_tag="ACIE68_003499"
     CDS             complement(11381..12148)
                     /locus_tag="ACIE68_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909759.1"
                     /translation="MQPSPAMQALIEQIYHIFRRYPVPQKFVVCCEYCLSQQEQKVLR
                     STSLRAISYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEWKILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWAFNNQSILKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(12151..12861)
                     /locus_tag="ACIE68_003500"
     CDS             complement(12151..12861)
                     /locus_tag="ACIE68_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909760.1"
                     /translation="MELVNTLFASLVGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDLTVQFTAMLNPDERVIIAKRPLKKCESWIEDIYIFQVFKDAW
                     KFHNDNSLRGFRDKQAELKLYARYYTVENCAEESCRDCNSCIRPSFSLSRSAIIRLNV
                     ANARFVYQPFTRDQRARG"
     gene            complement(12876..>14130)
                     /locus_tag="ACIE68_003501"
     CDS             complement(12876..>14130)
                     /locus_tag="ACIE68_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFX5909761.1"
                     /translation="IAVWRQCLFLVPALLTLGVWIIALHLADYQFRQMGAGWLADLML
                     PWLGVLLASLVGGEYWWLVIIPVGAHISFSLGYGWPTRYPLTGTSGLRCRNSLLFILL
                     MLGFVAGYQAYLYKQLNPGVGVRENIDTWAWRPDKLNNQLTPLRGKPQIQFTQNWPRL
                     DGATAAYPIYASAFYALSVLPEDFHEWEYLANSRTPEAYNKIVKGNADIIFVAQPSGG
                     QKKRAEESGVTLIYTPFAREAFVFIVNVDNPVNSLTEQQVRDIFSGAITNWRTVGGND
                     QEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQETEVASMMEGMIKVVAEYRNTNNAIG
                     YTFRYYATQMNADKNIKLLAINGIAPTAENIRNGKYPYIVDAFMVTRENMTSETQKLV
                     EWFLTPQGQSLVEDVGYVPMYKTLH"
BASE COUNT         3523 a         3772 c         3508 g         3327 t
ORIGIN      
        1 tttattaaga tcatcttttt agatggcact ttcatcagga atgaagaaga aacccttgct
       61 taaatgaatc tgatgaacat aagggaaacc agtattcacg ctggatcagc gtcgttttag
      121 gtgagttgtt aataaagatt tggaattgtg acacagtgca aattcagaca cataaaaaaa
      181 cgtcatcgct tgcattagaa aggtttctgg ccgaccttat aaccattaat tacgaagcgc
      241 aaaaaaaata atatttcctc attttccaca gtgaagtgat taactatgct gattccgtca
      301 aaactaagtc gtccggttcg actcgaccat accgtggttc gtgagcgcct gctggctaaa
      361 ctttccggcg cgaacaactt ccggctggcg ctgatcacga gtcctgcggg ctacggaaag
      421 accaccctca tttcccagtg ggcggcaggc aaaaacgata tcggctggta ctcgctggat
      481 gaaggtgata accagcaaga gcgtttcgcc agctatctca ttgccgccgt gcagcaggca
      541 accaacggtc actgtgcgat atgtgagacg atggcgcaaa aacggcaata tgccagcctg
      601 acgtcactct tcgcccagct tttcattgag ctggcggaat ggcatagccc actttatctg
      661 gtcatcgatg actatcatct gatcactaat ccagtgatcc acgagtcaat gcgcttcttt
      721 attcgccatc aaccagaaaa tctcaccctg gtggtgttgt cacgcaacct tccgcaactg
      781 ggcattgcca atctgcgtgt tcgtgaccaa ctgctggaaa ttggcagtca gcaactggca
      841 tttacccatc aggaagcgaa gcagtttttt gattgccgtc tgtcatcgcc gattgaagcc
      901 gcagaaagca gtcggatttg cgatgacgtt tccggttggg cgacggcact acagctaatc
      961 gccctctccg cccggcagaa tacccactca gcccataagt cggcacgccg cctggcggga
     1021 atcaatgcca gccatctttc ggattatctg gtcgatgagg ttttggataa cgtcgatctc
     1081 gcaacgcgcc attttctgtt gaaaagcgcc attttgcgct caatgaacga tgccctcatc
     1141 acccgtgtga ccggcgaaga aaacgggcaa atgcgtctcg aagagattga gcgtcagggg
     1201 ctgtttttac agcggatgga tgataccggc gagtggttct gctatcaccc gctgtttggc
     1261 aacttcctgc gccagcgctg ccagtgggaa ctggcggcgg agctgccgga aatccaccgt
     1321 gccgccgcag aaagctggat ggcccaggga tttcccagcg aagcgattca tcatgcgctg
     1381 gcggcaggcg atgcgctgat gctgcgcgat attctgctta atcacgcctg gagtttgttc
     1441 aaccatagcg aactgtcgct gctggaagag tcgcttaagg ccctgccgtg ggacagtttg
     1501 ctggaaaatc cgcagttagt gttattgcag gcgtggctaa tgcaaagcca acatcgctat
     1561 ggcgaagtta acaccctgct agcccgtgct gaacatgaaa tcaaggacat cagagaaggc
     1621 accatgcacg cagaatttaa cgctctgcgc gcccaggtgg cgattaacga tggtaatccg
     1681 gatgaagcgg aacggctggc aaaactggca ctggaagagc tgccgccggg ctggttctat
     1741 agccgcattg tggcaacctc ggtgctgggt gaagtgctgc actgcaaagg cgaattgacc
     1801 cgctcactgg cgctaatgca gcaaaccgaa cagatggcac gccagcacga cgtctggcac
     1861 tacgccttat ggagtttaat ccagcaaagt gaaattctgt ttgcccaagg gttcctgcaa
     1921 accgcgtggg aaacacagga aaaagcgttc cagctgatca acgagcagca tctggaacag
     1981 ctgccgatgc atgagtttct ggtgcgcatc cgagcacaac tgttatgggc ctgggcacgg
     2041 ctggatgaag ccgaagcgtc ggcgcgtagc gggattgaag tcttgtcgtc ttatcagcca
     2101 cagcaacagc ttcagtgcct ggcaatgttg attcaatgct cgctggcccg tggtgattta
     2161 gataacgccc gtagccagct gaaccgtctg gaaaacctgc tggggaatgg caaatatcac
     2221 agcgactgga tctctaacgc caacaaagtc cgggtgattt actggcaaat gaccggcgat
     2281 aaagccgccg ctgccaactg gttgcgtcat acggctaaac cggagtttgc gaacaaccac
     2341 ttcctgcaag gtcaatggcg caacattgcc cgcgcacaaa tcttgctggg cgagtttgaa
     2401 cctgcggaaa ttgttctcga agaactcaat gaaaatgccc gcagtctgcg gttgatgagc
     2461 gatctcaacc gtaacctgtt gctgcttaat caactgtact ggcaggccgg acgtaaaagt
     2521 gacgcccagc gcgtgttgct ggacgcatta aaactggcga atcgcaccgg atttatcagc
     2581 cattttgtca tcgaaggcga agcgatggcg caacaactgc gccagctgat tcaactcaat
     2641 acgctgccgg aactggaaca gcatcgcgca cagcgtattc tgcgagaaat caatcaacat
     2701 catcggcata aattcgccca tttcgatgag aatttcgttg aacgtctgct aaatcatccg
     2761 gaagtgccag aactgatccg caccagcccg ttgacccaac gtgaatggca ggtcctgggg
     2821 ctgatttact ccggttacag caatgaacaa attgcgggcg aactggaagt ggcggcaacc
     2881 accatcaaga cgcatatccg caatctgtac caaaaactcg gcgtcgccca ccgtcaggct
     2941 gccgtgcagc acgcgcagaa actgctgaag atgatgggtt atggggtgtg agtttagccg
     3001 gataatgcat tggatccggt ttacatcttt acatcattca atactcaccc gcgttacgcc
     3061 atctgtttct atcaaaccaa accgcaccgg caagaaacgc tccaccaccg cgatattggt
     3121 cagcagatgg catgaaggat gggcgacctt aaattctccc gcgcccgcca gcgccatcgg
     3181 cagtaccagt tggtcggcga gatattcccc caccgccgct gggcttgcca ggtagcgttt
     3241 cacctctttc actaactgcg cggctaccac ctcggcactg acgcgctttt caccgacgac
     3301 gaaaaagcgt tcagtgatat tttcactttc gacttccagc gagacggtat tacccggccc
     3361 ctggtcgcgc ggcaggttat gaatattctg ttcatgcagg gaaaaactcc ccgccagtgt
     3421 agcgatttca cgctcagcaa catggcgcgg cacaccagct aatagaactt ctccacgcat
     3481 ctgcacaatg ttcccgcgct cgccaagttg caaggtgtta aacgatgcca cgggcgagac
     3541 ttccgttgcc accacgccgc ctccggcagg ataaaaaccg tgacgtaaca gcgtggtttg
     3601 ctgatgaatt cctattttcg ccagcagcgg ctccagcacc cggcggataa aatccgcagg
     3661 cggggccgac gggttatcgg tgcctccgct cacttcaaca cgcgaaggtc catcggcaaa
     3721 ccacagcgcg ggcagcaccg tttgcagcac cagcgtacag cttccggcac taccgatagc
     3781 aaagcggtac tcgccgccgc gcacggtgcc aggccggaag accagacgct gcgaccccag
     3841 ctccgcgcct tccaccgttg ccctacaaat ttccgcagcc gcttttaccg cggtcagatg
     3901 ctggcgcaac agccccggtt tcgctcgccc ggcacgaatg ctggtgatgg taaatggctg
     3961 gccggttatc atcgacaggc tcagcgccga gcgcaggatc tgcccgccac cttcgccctg
     4021 tgcgccatcc agcgcaatca tccttttcat catttatcct tttacgcaca ccacctgacg
     4081 cagggtatag ataacttcca ccagatcgct ttgtgccgcc atcaccgcat caatatcttt
     4141 atacgccatc gggatttcgt cgatcacttc ggcatcttta cggcattcca catgcgcggt
     4201 ggcacgaatt tgatcttcca cgctgaacag ttttttcgct ttagtacggc tcattacccg
     4261 cccggcaccg tggctgcacg aacagaacga ctcttcattt cccagcccac ggacgataaa
     4321 gcttttcgct cccatcgaac cgggaataat tccatattga ccagcacgcg cagacaccgc
     4381 gcctttacgc gtcacgtaga tctcttcgcc aaagtgctgt tctttttgca catagttgtg
     4441 gtgacagttg atctcttcca tcgccagcgt ttgctgttgt cttaccgttt tctgcgtaac
     4501 gctctgcaac gctgttacta cgttttccat cattgcatcg cggttaaggc tggcaaaaag
     4561 ctgcgcccag gccaccgctt tcaggtaatc atcaaagtat tccgtacctt ccataaagta
     4621 cgccagatca cgcgacggca acgtttcaag ctggtcctgc atctcttttt gcgccagatc
     4681 gataaagtaa gtcccgatgg cgttaccaat tccgcgtgaa ccggagtgca gcataatcca
     4741 cacctgatcc gactcatcaa gacagatttc aataaaatgg ttacccgttc ccagcgttcc
     4801 cagatgttta tagttattgg tattcaggaa acgcggatat ttttgcgtta accactgata
     4861 accggcttcc agctcagccc atttagcatc gacgttaaca ggtggatttt cccaggcacc
     4921 tttatcacgt ttacaacggc cagtggtacg cccgtgcggc acggccgttt caatcgcctg
     4981 acgcagctct gccaggtttt caggcaggtc ttccgccgtt aacgcggtac gcagcgcgtt
     5041 cattccacag ccaatatcca cgcccaccgc cgccggaata atcgcccctt tggtcgggat
     5101 cacgctacca atggtggaac ctttacccag gtgtacatca ggcattaccg caatatgttt
     5161 gaaaataaac ggcatcttcg ccgtattaat aagttgctga cgcgcatcgg cctctaccgg
     5221 cacgcctttg gtccacattt taccggggca ttttcagtgg tcagtaattc gtaattcatt
     5281 ttgtttttct cttttcgttg tttgctgtcc tgataattgc aaccgtcgtg ccagaaaatt
     5341 aaataagcag cttaattttt taattcattg ttttaaaaaa gattatatct ttacgtccgt
     5401 aaccggagat ttcccgcaaa gccaatttac cgataatgaa atatcgtctt ttataaggat
     5461 atctaagatg cgtaaaacag tggcttttgg ctttgtcggt accgtactgg attatgccgg
     5521 gcgcggcagt cagcgctggt caaaatggcg tccgacactc tgtttatgcc agcaagaatc
     5581 gttggtcatc gatcgactgg aattgttgca cgacgcccgc tcgcgctcgc tatttgaaac
     5641 gcttaaacgc gatatcgcca gcgtttcgcc agaaacagaa gtggtgagcg ttgagattga
     5701 actgcataac ccgtgggatt tcgaagaggt ctacgcctgc ctgcatgatt tcgcccgtgg
     5761 ttacgagttt cagccagaaa aagaagacta tttaattcac atcaccaccg gtacccacgt
     5821 cgcgcagatt tgctggtttc tgctggcaga agcgcgttac ctgcccgccc ggctgataca
     5881 atcttcacct ccgcgcaaaa aagaacagcc gcgcggcgca ggtgaagtga cgattatcga
     5941 tctcgattta agccgttata acgccatcgc cagccgcttt gccgaggaac gccagcaaac
     6001 gcttgatttt cttaagtccg gcattgccac gcgtaccccc cacttcaacc gcatgattga
     6061 gcagatcgaa aaagtggcga tcaaatcccg cgcgccgatt ctgcttaacg gtccaaccgg
     6121 cgcgggcaag tcatttctgg cgcgacgcat cttcgagtta aaacaggcgc ggcatcagtt
     6181 tagcggcgcg tttgtggaag tgaactgcgc caccctgcgc ggcgataccg ccatgtcgac
     6241 gctgtttggt catgtaaaag gcgcgtttac cggggcgcgg gaatctcgtg aaggtttatt
     6301 acgcagcgcc aacggcggaa tgttgtttct tgatgagatt ggcgaactgg gcgcagacga
     6361 acaagcaatg ctgctaaaag ccattgaaga gaaaaccttt tacccgtttg gcagcgatcg
     6421 ccaggtgagc agtgattttc agcttatcgc cggaacggtg cgcgatttgc gccagctggt
     6481 tgccgaaggc aaatttcgcg aagatctgta cgcgcggatc aatctctgga ccttcaccct
     6541 gccgggtcta cgccagcgcc aggaagatat tgaaccgaac cttgattatg aagtggagcg
     6601 ccacgccaca ctcactggcg acagcgtgcg ttttaacacc gaagcgcggc gcgcctggtt
     6661 ggcttttgcg acctctcccc aggcaacatg gcgcggtaac tttcgcgaac tttctgccag
     6721 cgtcacgcgg atggccacct ttgccactag cggacgcatc actctggacg tggttgaaga
     6781 tgagataaac cgtctgcgct ataactggca ggagagccgc ccctccgcgc ttacggcgtt
     6841 gctgggcgca gaggcagaaa acatcgatct cttcgaccgt atgcaactgg aacacgtgat
     6901 agctctctgt cgccaggcaa agtcgctttc cgctgccgga cgcctgcttt ttgatgtttc
     6961 gcgccagggc aaagccagcg tcaatgacgc ggatcggcta cgcaaatacc tggcgcgttt
     7021 tggtctgacg tgggaagccg tgcaggatca gcacagctcc agttgaatat gatggtccgt
     7081 cagcacctgc attacgctta ctggcggcgg ggcgtcggtg tagacggcat ctaccatgct
     7141 gatgctgccc atattgacca tcgcgttacg gccaaatttc gagtgatcga caaccagcat
     7201 aacgtggcgc gagttctcaa taatggcgcg cttggtgcga acttcgtggt aatcgaactc
     7261 cagcagcgag ccgtcgctat cgatgccgct tatccccaga atgccgaaat caaggcggaa
     7321 ctgggagata aaatcgagcg tcgcttcgcc aatgatcccg ccatcgcggc tgcgtaattc
     7381 gccaccggcg agaataatgc ggaaatcttc ttttaccatc aacgtgttag caacattgag
     7441 attgttggtg acaatacgca aattgctgtg attaagcagc gcgtgcgcca ccgcttccgg
     7501 cgtggtgccg atatcgataa acagtgtcga gccgttgggg atttgctccg ccactttgcg
     7561 ggcaatcctc tctttttctt cggtctgggt ggctttgcga tcgtgccacg gcgtgttaac
     7621 cgaactggaa ggcagcgccg caccgccatg gtgacgcagg atcaggtttt gctccgccag
     7681 ctcattgagg tcgcggcgaa tagtctgcgg gctgacggag aaatgctcta ccagctcttc
     7741 agtactgaca taaccctgct gtttaaccag ttcgataata ccgttgtgac gttgtgtttg
     7801 tttcatttat aaatccctgg aattattttc gttttcgcgc attgagcgaa tcaacaaaag
     7861 ccatcgctaa acccacggct aacccggcga tgtgtgctcc gttcgccatc gacatcccaa
     7921 acaaatcaaa ccatccggcg acaatccaga tcagcgcaaa gataattaac ccacgttgca
     7981 ggtaaatgcc actttgcgga tcgcgttcac cacgtagcca gacgtagccc atcagcgcat
     8041 acaccacgcc agaaagcccg ccaaaccacg gcccgctgaa tttttgctgc acatagccgc
     8101 ttaacagggc gctaatgaga gtaataacaa ttagcttacc gctaccgagg cgtttttcca
     8161 ccgcaccgcc gagataccac caccagagca ggttaaagag gatatgcatc agcgagaagt
     8221 gcattaacgc gtgggtgaag taacgccaga actcaaattt cagtgttgga tcgaatggcc
     8281 aggccagcca taacatcact tcctgatcgc cgagaatttg catggcgata aacaccacca
     8341 cgcaggcgat catcaccacc caggttaccg gacctgcgcg ttcacgcaag gcagcaaaga
     8401 aaggataacg gcgataatgc agaccactgc cggtatggcc cgcctgccag ctggccgcca
     8461 gataacgcgg atctgccggg ttttcgagaa aacgcgccag ctccgcccgc acgcgctcgg
     8521 cctgggactc atccgccagc cagacatcgc tttggttatg ttgttgaatc gtaaggataa
     8581 caccctgcgt cgccatgtaa tcaacaaacg cctgcgccac gcgggggtta gcaaaagagg
     8641 taatcatcaa catcgttgct gtcgcttatt ccacacaaaa ggggacagta taaagcgtta
     8701 cgcgccgtac gccacctctg cgggaaactg acgctgccag gcttcaaaac cgccgtcaat
     8761 gctatagacc acatcgtagc cctgttgcag cagatactgc gccgcgcctt tgctgctatt
     8821 gccgtgataa cacatcacca tcaccggagt gtcaaagtcg ttatcacgca taaacgcgcc
     8881 cagcgtgtcg ttggttaaat ggaaagcctg caccgcatgc cccattgcga aactctgtgg
     8941 atcgcgaata tcgaccagca ccgcctcttt ttcctgcaac ttctggtgcg cgacggcaac
     9001 gttaatacat tcgaactgat ccatgcgtct ctctttcttt acaaacaagt gggcaaattt
     9061 accgaacagt ttacgtcgaa gcggcagata aacgccataa tgttatacat atcactctaa
     9121 aatgtttttt caatgttacc taaagcgcga ttctttgcta atatgttcga taacgaacat
     9181 ttatgagctt taacgaaagt gaatgagggc agcatggaaa ccaaagatct gattgtgata
     9241 gggggcggca tcaatggtgc tggtatcgcg gcagacgccg ctggacgcgg tttatccgtg
     9301 ctgatgctgg aggcgcagga tctcgcttgc gcgacctctt ccgccagttc aaaactcatt
     9361 cacggtggcc tgcgctacct tgagcactat gaattccgcc tggtcagcga ggcgctggct
     9421 gaacgtgaag tgctgctgaa aatggctccg catatcgcct tcccgatgcg ttttcgcctg
     9481 ccacatcgtc cgcatctgcg cccggcgtgg atgattcgca ttggtctgtt tatgtacgat
     9541 catctgggta aacgcaccag cctgccggga tcaactggtt tgcgttttgg cgcaaattca
     9601 gtgttaaaac cggaaattaa gcgcggattc gaatattctg actgttgggt agacgacgcc
     9661 cgtctggtac tcgccaacgc ccagatggtg gtgcgtaaag gcggcgaagt gcttactcgg
     9721 actcgcgcca cctctgctcg ccgcgaaaac ggcctgtgga ttgtggaagc ggaagatatc
     9781 gataccggca aaaaatatag ctggcaagcg cgcggcctgg ttaacgccac cggcccgtgg
     9841 gtgaaacagt tcttcgacga ggggatgcat ctgccttcgc cttatggcat tcgcctgatc
     9901 aaaggcagcc atattgtggt gccgcgtgtg catacccaga agcaggccta cattctgcaa
     9961 aacgaagata aacgtattgt gttcgtgatc ccgtggatgg acgagttttc catcatcggc
    10021 accaccgatg tggaatacaa aggcgatccg aaagcggtga agattgaaga gagtgaaatc
    10081 aattacctgc tgaaagtgta taacacgcac tttaaaaagc agttaagccg tgacgatatc
    10141 gtctggacct actccggtgt gcgtccgctg tgtgatgatg agtccgactc gccgcaggct
    10201 attacccgtg attacaccct tgatatccat gatgaaaacg gcaaagcacc gctgctgtcg
    10261 gtattcggcg gtaagttgac cacctaccgc aaactggcgg aacatgcgct ggaaaaactg
    10321 acgccgtatt atcagggcat tggcccggcg tggacgaaag agagcgtgct gccgggtggt
    10381 gccattgaag gcgaccgcga cgattatgcc gctcgcctgc gccgccgcta tccgttcctg
    10441 actgaatcgc tggcgcgtca ttacgctcgc acttacggca gcaacagcga gctgctgctc
    10501 ggcaatgcgg gaacggtaag cgatctcggg gaagatttcg gtcatgagtt ctacgaagcg
    10561 gagctgaaat acctggtgga tcacgaatgg gtccgccgcg ccgacgacgc cctgtggcgt
    10621 cgcacaaaac aaggcatgtg gctaaatgcg gatcaacaat ctcgtgtgag tcagtggctg
    10681 gtggagtata cgcagcagaa gttgtcgctg gcgtcgtaag gatgatgccc ggttgtgaag
    10741 tgcaaccggg ctatttggat tccttagact attcattgta aataattgaa taacggcccc
    10801 tgagctaaaa tcgccaaact taagcacgtt acccaatcgc cccacttctt cctctggttt
    10861 ctattttttc tgcaccaatg gcaataatat cccaatgcaa aaccaagctg tgcactaatc
    10921 gggccgacaa aatcagtcca aggctgcggc caaaaagggg aaaaattcag gggaagccag
    10981 acaaacgtca gaaagaaatt tcggatattt tccagcaacg ctgatttctc tggagaaata
    11041 taaagaatga aattcgagag aattaaccag aaatagaccg gcgaaaagac cagccagcaa
    11101 tgcagtctgt tttttaattc aggaggcgtt ctgcgggaga attgccaacc gatgccagcc
    11161 attacactga aaaaaagtgc atattcaacc attaactcaa gttgtagttc ctgaagagaa
    11221 atacgcacaa ccaggagcgg aaaggagaat attatttctg ccagaaaagt taacgtgaat
    11281 ataagtacgg ctaagacaat aaccgtcggc cataccaata atggcacgat aacaaaaccg
    11341 agaattacag ggggaatata aagccggaat ttaatcattt ttattttcct ggtgattcgg
    11401 atgatgcgtc atacatcgcg cataaacact catcaattat ccagtcgtct acccagacct
    11461 gatgctgtgt gccctgttct ggctgctttg gtgggttctc aatcgcctgc cgcgcgcgtt
    11521 ctttcaagat ggattgattg ttgaaggccc aggcattgat ttgggtggta atgacgttat
    11581 cgatatcgtc ctggttatct ctgatataat cctcacaata tctgttcaat aaacaggcaa
    11641 gagaaacggt cgaccaaaag ccgggaactg aattaatggc atccagcaat ggcgcaagag
    11701 cgatatcggc acggaaaaac atttccagca tatactgtaa ttcgacagct tcatcgccgg
    11761 atacccaatc agtcatatac tggcaggcaa atcgttgcaa aattttccat tcatcctcac
    11821 gccaattttc tttactggca aggtttatac gccgtagaga aaatacttcg tgaatattgt
    11881 caaactgccc ctgtgcaaca aattccagta aacgcggcag aaaatagcgg acctcatcgc
    11941 tattttgcgg atcaggccct gggctactat tccatgcatt gattaaggaa tacgagatcg
    12001 ctcgtaacga tgtactccgt aatacctttt gttcctgctg actcaggcaa tattcacaac
    12061 aaaccacaaa tttctgcggc accggataac gacgaaaaat atgataaatc tgctcaatta
    12121 atgcctgcat ggcggggctg ggttgcataa ttaccccctc gcccgctgat cgcgtgtaaa
    12181 cggttgataa acaaaacgag cattcgccac gttcaggcga ataatggcac tacgagagag
    12241 ggaaaagctg gggcgaatgc aactattgca gtctcgacag ctttcttcag cgcaattttc
    12301 cactgtgtaa taacgagcgt agagtttcag ttccgcttgc ttatcacgaa atccgcgtaa
    12361 actgttatcg ttatgaaatt tccaggcatc tttaaagacc tgaaaaatgt agatatcctc
    12421 aatccaggac tcacattttt taagcgggcg ctttgctatg atcacccgct catccgggtt
    12481 tagcatcgcc gtaaattgaa cggtgaggtc atttgtcgtg ttcagatcac attgaacatc
    12541 tggtacatca ctgatatagg ggtcaaatcc aaagtcagca ccacaggcac aaagacggct
    12601 aatggtggcg gtatcgaggg aaaatcccgg ccatacattg ctggacgtta agccaatatt
    12661 taatgtgaca ttatatcgaa gcaatccctc aagaccgtca gcaccgacga acttttcccc
    12721 ttcatccagg gcctggttaa tgagttgctg aacaatgcct tcatcccagt atgccgattt
    12781 gcagttggca atcgtaatat ccacgcctgt gaatggatca gtaccgacaa ggcttgcaaa
    12841 taatgtatta actaattcca tttttattat ccgccttaat gcaacgtttt atacatcggc
    12901 acataaccta catcttccac caggctctgc ccctgcggcg ttaaaaacca ctccaccaat
    12961 ttttgtgttt ctgacgtcat attttcccgc gttaccataa aggcatcgac gatataggga
    13021 tatttgccgt tgcgaatgtt ttcggcagtc ggtgcaatac cgttaatcgc tagcagtttt
    13081 atatttttat cggcgttcat ttgtgttgcg taataacgga aggtgtaacc tattgcgttg
    13141 tttgtattac ggtattcggc aacgacttta atcattccct ccatcatact tgccacttct
    13201 gtttcctgcg gcgagatcat gcggacattt ttcatgacct gggattgcat caccgtctgg
    13261 ctaccagagt cttccgggcg ttgccaggtc tggatctcct gatcgttacc gccaaccgtg
    13321 cgccagttgg taattgcacc actgaagatg tcacgcactt gttgttcggt cagggaatta
    13381 accgggttat ctacattgac gatgaaaaca aacgcttcac gggcaaaagg cgtgtaaatc
    13441 aaagtgacgc ccgattcctc cgcgcgtttt ttctgcccac cggaaggttg agccacaaag
    13501 ataatatcgg cattaccttt aacaatcttg ttatatgctt cgggagtacg agagttcgcc
    13561 agatattccc attcgtgaaa atcttccggc aaaacgctta gtgcataaaa ggcagaggca
    13621 taaatggggt acgccgccgt tgcgccatca agtcgcggcc agttttgcgt gaactgaatt
    13681 tgcggtttac cacgcagtgg tgtcagttga ttattgagtt tatcgggtcg ccaggcccag
    13741 gtatcaatat tttcacgcac accgacgccg ggattaagct gtttatataa ataagcctgg
    13801 taaccggcga caaaaccaag catgagaagg ataaacaaga gagaattacg gcaacgtaac
    13861 ccggacgtgc ccgttaaagg atatctggtc ggccagccgt accccagcga aaaactgata
    13921 tgcgcgccaa caggtataat gactaaccac cagtactcac caccgactaa tgaggctaac
    13981 aaaacgccca gccaggggag cataagatca gccaaccaac ccgctcccat ctggcgaaat
    14041 tgataatctg ccagatgcag cgcgattatc cagaccccca gggttaataa tgcgggtact
    14101 aaaaaaagac attgtcgcca gacggctatt
//
LOCUS       DBANUU010000115        14024 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000115 DBANUU010000000
VERSION     DBANUU010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.115"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1141)
                     /locus_tag="ACIE68_003502"
     CDS             complement(<1..1141)
                     /locus_tag="ACIE68_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFX5909762.1"
                     /translation="MSTKFYTLLTDIGAAKLASAAALGVPLKITHMAVGDGGGVLPTP
                     DAKQTALVNEKRRATLNMLYIDPQNSSQIIAEQVIPENEGGWWIREVGLFDESGALIA
                     VGNCPESYKPQLAEGSGRTQTVRMVLITSSTDNITLKIDPAVVLATRKYVDDKVLELK
                     VYVDDQMAKHLAAPDPHSQYAPKASPTFTGTPKAPTPAAGNNTTQVATTAFVQAALTA
                     LINGAPATLDTLKEIAAAINNDPNFSTTINNALALKAPLSSPALTGTPTAPTAAQSVN
                     NTQIATTAFVKSAIAAMVGSAPAALDTLNELAAALGNDPNFATTMLNALAGKQPLDNT
                     LTNLSGKDVAGLLAYLGLGDTSGYVGRWVNTRVFTSSGTYTPTPGT"
     gene            complement(1138..1743)
                     /locus_tag="ACIE68_003503"
     CDS             complement(1138..1743)
                     /locus_tag="ACIE68_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFX5909763.1"
                     /translation="MNSLLPPGSTSLERRLAQTCSGISDLQVPLRDLWNPATCPVSFL
                     PYLAWAFSVDRWDEGWTESVKRQVVKDAFYIHQHKGTTSAVRRVVEPFGFLIRIIEWW
                     QTGETPGTFRLDIGVQDQGITEDTYLELERLISDAKPCSRYMIGMSINLQTSGPHWVG
                     AASYLGEEITIYPYINETIISSGTAHEGGAVHVIDTMRVNP"
     gene            complement(1736..2644)
                     /locus_tag="ACIE68_003504"
     CDS             complement(1736..2644)
                     /locus_tag="ACIE68_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFX5909764.1"
                     /translation="MAVIDLSQLPAPQIVDVPDFETLLAERKAEFVALHPKDEQEAVM
                     RTLELESEPVTKLLQENAYRELLLRQRINEAAQAVMVAYAIGGDLDQIAANYNVKRLT
                     VTPADDDAVPPVAAVMESDEALRLRVPAAFEGLSVAGPTAAYEFHARSADGRVADASA
                     TSPAPAEVVLTVLSREGDGTAEKDLLDVVEKALNSENVRPVADRLTVRSAEIIPYRVE
                     ATIFLYPGPEAEPVMAAAKASLQKYIASQTRLGRDIRRSAIFAALHVEGVQRVELASP
                     LADVVLNKTQAASCTQWSVTNGGTDE"
     gene            complement(2631..2990)
                     /locus_tag="ACIE68_003505"
     CDS             complement(2631..2990)
                     /locus_tag="ACIE68_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFX5909765.1"
                     /translation="MTLYSGMNNTSGKAITDIDHLRQSVRDILLTPQGSRIARREYGS
                     LLSALIDQPQNPALRLQVMSAVYVALSRWESRLTLDSITINSNFDGSMVVALSGRRNN
                     GVPVSLSVSTGAENGSD"
     gene            complement(2987..3565)
                     /locus_tag="ACIE68_003506"
     CDS             complement(2987..3565)
                     /locus_tag="ACIE68_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFX5909766.1"
                     /translation="MNAQLTEIMRLITNLIRTGVVTEVDRENWLCRVKTGELETNWIS
                     WLTLRAGNARTWWRPSEGEQVVLLSLGGNLETAFALPAIYSNQFAPPSTSADACVTEH
                     PDGGWFEYEPATGRWYISGIKSMVIEAADNITLKTSEFVLEADRTRINSEVVINGGVT
                     QGGGAMSSNGIVVDAHQHTGVLKGGDTTGGPV"
     gene            complement(3634..4080)
                     /locus_tag="ACIE68_003507"
     CDS             complement(3634..4080)
                     /locus_tag="ACIE68_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFX5909767.1"
                     /translation="MAELQKVDDWLSALLANLEPVTRSRMMRQLAQELRRTQQQNIRM
                     QRNPDGSSYEPRRVTARSKKGRIKRQMFAKLRTTKYLKTTASAGSASVQFEGKVQRIA
                     RVHHYGLRDRVSRKGPEVRYAERRLLGVNDDVEAMTRDMILQWLAG"
     gene            complement(4073..4504)
                     /locus_tag="ACIE68_003508"
     CDS             complement(4073..4504)
                     /locus_tag="ACIE68_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFX5909768.1"
                     /translation="MNKPKSLRHALNKAVPYVRNNPDKLHLFVDNGSLVATGACSMSW
                     EYRYTLNVVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPALREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTDGSVSSVEAVAEPDESEEMWTVKRG"
     gene            complement(4467..4715)
                     /gene="lysC"
                     /locus_tag="ACIE68_003509"
     CDS             complement(4467..4715)
                     /gene="lysC"
                     /locus_tag="ACIE68_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461641.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFX5909769.1"
                     /translation="MLILSGCGNAPHSPEVQLTVSGCPRVTPCRLGQAAPRTNGDLNA
                     LLDETEAAWAVCADKVDMIIACQERNSEQTKIPAPRPQ"
     gene            complement(4600..5028)
                     /gene="lysB"
                     /locus_tag="ACIE68_003510"
     CDS             complement(4600..5028)
                     /gene="lysB"
                     /locus_tag="ACIE68_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341071.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFX5909770.1"
                     /translation="MNRLLLVVLALLLAALGWQTWRLADASQTISTQADELRSKSQAL
                     AKSNSQLISLSILTEANNREQARLYAEAEQTSVLLRQRQHRIEELKRENEDLRRWADT
                     PLPADIIRLRERPTLTRGAAYRQWLSASDAVSAGAGSAAH"
     gene            complement(5025..5402)
                     /locus_tag="ACIE68_003511"
     CDS             complement(5025..5402)
                     /locus_tag="ACIE68_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFX5909771.1"
                     /translation="MKKKLISGLFLMLWMALLIAAMVYPQGIFPVLAASGVWVACLLT
                     WGAITVALAALIKNGPLWQELRASLLKTITRKENVFISWVMRLLIVVSLAWTGWAITL
                     VFYLLTVIAFWITRNQMAQQVAA"
     gene            complement(5404..5916)
                     /locus_tag="ACIE68_003512"
     CDS             complement(5404..5916)
                     /locus_tag="ACIE68_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFX5909772.1"
                     /translation="MNPSIVKRCLVGAVLAIAATLPGFQQLHTSVEGLKLIADYEGCR
                     LQPYQCSAGVWTDGIGNTSGVIPGKTITERQAAEGLISNVLRVERALERCVKQQPPQK
                     VYDATVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAR
                     EMAWCLQGAN"
     gene            complement(5897..6112)
                     /locus_tag="ACIE68_003513"
     CDS             complement(5897..6112)
                     /locus_tag="ACIE68_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HP1 family phage holin"
                     /protein_id="HFX5909773.1"
                     /translation="MTLERISAFITYCIAVVLAWLGDLSIKDASTLGGLMIGVLMLAI
                     NWYYKHKAYQLLRDGQISREDYESINR"
     gene            complement(6116..6319)
                     /locus_tag="ACIE68_003514"
     CDS             complement(6116..6319)
                     /locus_tag="ACIE68_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFX5909774.1"
                     /translation="MKVRAHQYDTVDALCWRHYGRTQGVTEQVLKANPGLAEFGPFLP
                     HGLQVELPDIPTTTTVQTVQLWD"
     gene            complement(6319..6783)
                     /locus_tag="ACIE68_003515"
     CDS             complement(6319..6783)
                     /locus_tag="ACIE68_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFX5909775.1"
                     /translation="MKFVAPEQAPEQAEIIRNTPFWPDVDLSEFRSVMRTDGTVTQPR
                     LKQVALSAISEVNAELYEFRRRQQMLGYASLAEVPAEQLDGKSERIQHYFNAVYCWAR
                     AMLNERYQDYDATASGVKRGEELAEASGDLWRDARWAICRVQDAPHCTVELI"
     gene            complement(6879..7529)
                     /locus_tag="ACIE68_003516"
     CDS             complement(6879..7529)
                     /locus_tag="ACIE68_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFX5909776.1"
                     /translation="MSLSPARQHRLRVQAEQAAREGGSVRHASGYDLMLLQLAEDRRR
                     LKGVQSTVKKAEIKVELLPKYAAWAEGVLAAGGAQQDDVLMYVMLWRIDAGDYAGALE
                     IGRHALRHGWVMPLGNRNVQTVLAEEMADAAQSAMLAATGFDADLLLQTLELTDGLDM
                     PDQSRARLHKAIGAVLSESNPASALNHLNHALQLDPRCGVKKDKQQLERRLRNDSR"
     gene            complement(7533..8591)
                     /locus_tag="ACIE68_003517"
     CDS             complement(7533..8591)
                     /locus_tag="ACIE68_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001714844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFX5909777.1"
                     /translation="MKKNTRFAFNAYLQQLARLNGVAVEELSSKFTVEPSVQQTLEDQ
                     IQQSAAFLTLINVTPVTEQSGQLLGLGVGSTIAGTTDTTAKEREPVDPTLMVDVEYKC
                     EQTNFDTVLTYAKLDLWAKFQDFQVRIRDAIVKRQALDRIMIGFNGVKRAKTSNRSEN
                     PLLQDVNKGWLQKIREDAPDHVMGSTTTGGETTPGAVKVGKGGEYANLDAVVMDAVNE
                     LIDVVYQDDDDLVVICGRELLSDKYFPLVNKEQENSEKLAADMIISQKRMGGLQAVRA
                     PFFPPNALLITRLDNLSIYWQEDTRRRSVIDNPKRDRIENFESVNEAYVVEDYRCAAL
                     VENIQIGDFSAAAAETGE"
     gene            complement(8608..9441)
                     /locus_tag="ACIE68_003518"
     CDS             complement(8608..9441)
                     /locus_tag="ACIE68_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFX5909778.1"
                     /translation="MTVKAKRFRIGVEGATTDGREIQREWLEQMAASYNPAVYTALIN
                     LEHIKSYLPDSTFNRYGKVTALFAEEITEGPLAGKMALYADVEPTESLVELVKKGQKL
                     FTSMEVSPKFADTGKAYLVGLAATDDPASLGTEMLTFSASAAHNPLANRKQNPANLFT
                     AAEETVIELEEIQDDKPSLFARVTALFTKKEQSDDARFSDVHKAVELVATEQQNLSAR
                     TEKSLSEQEERLSELETALQEQQTAFNELVNKLSHEDSRQDYRQRATGGNAPADTLTN
                     C"
     gene            9584..11350
                     /locus_tag="ACIE68_003519"
     CDS             9584..11350
                     /locus_tag="ACIE68_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFX5909779.1"
                     /translation="MNTTLTPADLDPRRQAMLLYFQGYRVARIAEMLGEKVATVHSWK
                     KRDKWGDYGPLDQMQLTTAARYCQLIMKEHKEGKDFKEIDLLARQSERHARIGKFNNG
                     GNEADLNPNVANRNKGPRRQPEKNVFTDEQIEKLEEIFHSSMFNYQRHWWEAGKTNRI
                     RNLLKSRQIGATFYFAREALIDALLTGRNQIFLSASKAQAHVFKQYIIDFAKEVEVEL
                     KGDPMVLPNGATLYFLGTNARTAQSYHGNLYLDEYFWIPKFQELRKVASGMAIHKKWR
                     QTYFSTPSSLTHSAYPFWSGALFNRGRNKADKVDIDLSHSNLAPGLLCADGQYRQIVT
                     VEDAVRGGCNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVW
                     TDFHALALRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGM
                     DFRAQADAIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVL
                     KAYDIISHRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATM
                     HALFNEPLQGESANTSNIVEIF"
     gene            11350..12378
                     /locus_tag="ACIE68_003520"
     CDS             11350..12378
                     /locus_tag="ACIE68_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFX5909780.1"
                     /translation="MGKSKKNRAAATKRSQHKNQTSAEAFSFGDPIPVLDRRELLDYV
                     ECVQTDRWYEPPVSFDGLARTFRAAVHHSSPIAVKCNILTSTYIPHPLLSQQAFSRFV
                     QDYLVFGNAYLEKRTNRFGEVIALEPALAKYTRRGLDLDTYWFVQYGMTTQPYQFTKG
                     SIFHLMEPDINQEIYGLPGYLSAIPSALLNESATLFRRKYYINGSHAGFIMYMTDAAQ
                     NQEDVNNLRNAMKSAKGPGNFRNLFMYSPNGKKDGLQIIPLSEVAAKDEFLNIKNVSR
                     DDMMAAHRVPPQMMGIMPNNVGGFGDVEKASRVFVRNELIPLQKRLQEVNEWIKEPIM
                     QFNEYNLA"
     gene            complement(12415..>14024)
                     /locus_tag="ACIE68_003521"
     CDS             complement(12415..>14024)
                     /locus_tag="ACIE68_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFX5909781.1"
                     /translation="TIFVGPNNSGKSRALIDIENQLTTRNAAQGEVIKQILIKPLSED
                     EAKKEIESLQVEPNQDEKHAGHVVLSRLNLRGEGISRILVYLDGMLREFQQPNEFNRE
                     HLYQYYSLLTLRLDGRSRLSLTSSQGSSDLQGEPTNNLSFLFKNNKARMELRDIVFDA
                     FGKYLVIDPTNIGYLRIRLSERAPETEQEERGWDEKSVLFHSKASLITEASDGVNAFV
                     GMMISLIVGSPKVTLIDEPEAFLHPSLCSKLGKEISRISAHSNKNVFISTHSANFLMG
                     CVQGKVPLNIVRLTFDGKSGTSRLLTKEQLTPLMRNPLLRSIGVLNALFYNYVVVTEA
                     DADRAFYQEINERLLAAGDPRGIEGCLFLNAQNKQTVWDIVKPLRELGIPCVGVVDID
                     ILKEGGVVWKKPMLGAFFPEMKHETLGLERQKLFQAFKNTNKNMKTDGGLSLLSGSDK
                     TACSEFFKSLSDYGVFVVPTGEIESWLQNLEIGRGKNTWLTSIFEKMGDDPNDESYVK
                     PSEGDVWDFIGNIKHWLVNPERKGIPE"
BASE COUNT         3150 a         4041 c         3513 g         3320 t
ORIGIN      
        1 ttgttcctgg cgtcggggtg tacgtacctg atgacgtgaa aacccgggta ttcacccagc
       61 gtcccacgta tccgcttgta tctcccaaac caaggtatgc gagaagacca gctacatcct
      121 ttccactcaa attagtcagc gtattgtcca gcggttgttt acctgccagc gcattaagca
      181 ttgtcgtggc aaagttcgga tcattcccca gcgccgccgc cagttcgttc agtgtatcca
      241 gtgccgcagg tgcagaaccc accattgccg caatcgccga tttcacaaaa gccgtagtgg
      301 caatctgtgt attgttgacc gactgcgccg ccgtgggggc tgttggcgtt ccggtgagtg
      361 ccggactcga tagcggcgct tttagtgcca gcgcattgtt aatggtggta ctgaaattcg
      421 gatcattgtt aatggctgcg gctatttctt tcagcgtgtc cagcgtggct ggcgcaccat
      481 taataagggc cgtcagtgcc gcctgtacaa acgcagtggt cgcaacctgc gtggtattat
      541 tccccgccgc tggcgttggc gctttggggg ttccggtaaa tgtcgggctg gcttttggcg
      601 cgtactgtga atgcgggtcc ggtgcggcaa gatgttttgc catctgatca tccacgtaca
      661 ccttcagctc cagtaccttg tcatccacat acttgcgggt tgccagcact acagcagggt
      721 cgattttcag ggtgatattg tccgtactgc tggtaatcag caccatgcgc acggtctggg
      781 tgcgcccgct gccttcagcc agttgcggct tatagctttc cgggcagttg cccacggcaa
      841 tcaatgcccc ggactcatca aacaagccca cttcacgtat ccaccaaccg ccctcgtttt
      901 cagggatcac ctgttcggca ataatctggc tgctgttctg cgggtcgata taaagcatat
      961 tcagcgtagc ccggcgtttc tcatttacca gtgccgtctg ctttgcgtcc ggcgttggca
     1021 atactccacc gccatcgccc accgccatat gggtaatttt tagcggcaca ccgagcgcgg
     1081 cggcgctggc aagtttcgcc gcgccaatat ccgtcagcag ggtataaaat tttgtgctca
     1141 tggattcact ctcattgtgt caataacatg gaccgccccg ccttcatgcg cggtgccact
     1201 ggaaataatc gtttcgttga tatacggata gatcgtgatt tcttcgccaa gatagctggc
     1261 ggctcccacc caatgcgggc cgctggtctg cagattgatg gacatgccga tcatgtagcg
     1321 gctacatggt ttggcatcgc ttatcagtcg ctcaagttcc agataggtat cttcagtgat
     1381 gccctggtcc tgcacgccga tatccaggcg aaacgtgccc ggtgtttctc cggtctgcca
     1441 ccactcaata atgcggatca ggaatccgaa cggttccacc acccgccgca cggcactggt
     1501 ggttccttta tgctgatgaa tataaaaagc atccttcact acctggcgtt tgacgctttc
     1561 tgtccagccc tcgtcccagc gatccacaga gaacgcccag gcgagataag gcaggaagct
     1621 gaccggacag gtagccggat tccacaagtc acgcagcggc acctgcaaat cagaaatccc
     1681 gctacaggtt tgcgccagtc ggcgctccag tgaagttgaa cccggtggca gcagactatt
     1741 catccgttcc tccgttggtt acgctccact gcgtacatga tgccgcctgt gttttgttca
     1801 ggaccacatc cgccagcgga gaagccagtt ccacacgttg aacaccctca acatgcagag
     1861 cagcaaagat ggcgctacgg cgaatatccc gaccaagcct cgtctggctg gcaatgtact
     1921 tctgcagact ggcttttgcc gctgccatta ccggctctgc ttccggtccc ggatagagaa
     1981 aaatggtggc ttccacgcga tacgggatga tttctgcgct gcgaaccgta agacggtcag
     2041 ccaccgggcg gacgttctca ctgttcagag ctttttccac cacgtccagc aggtcttttt
     2101 ctgcagttcc atcgccttcg cggctaagga cagtcagcac cacctctgca ggtgccgggc
     2161 tggttgcact ggcatccgcc acccgaccgt cggcgcttcg ggcatgaaat tcataagctg
     2221 cagttggccc cgcaactgaa agtccttcaa aggctgcagg cacacgcagg cgtaacgctt
     2281 catcactttc catcacagcc gcaacaggcg gtacagcgtc atcatcagca ggtgtcaccg
     2341 tcaggcgttt cacgttgtag ttggcagcaa tttggtcaag gtctccgcca atcgcgtaag
     2401 ccaccatcac cgcctgcgcg gcttcgttaa tgcgctgacg cagaagcaat tcacggtaag
     2461 cgttctcctg caacaattta gtgacgggtt cagattcaag ctccagcgta cgcattactg
     2521 cttcctgctc atctttcgga tgaagcgcca caaattctgc cttgcgttca gcaagcagcg
     2581 tctcaaagtc cggcacatcc acaatctgcg gcgcaggcaa ctgcgaaagg tcaatcactg
     2641 ccattctctg ctcctgttga tacggaaagg gaaacaggca caccgttatt acgccgcccg
     2701 ctcagcgcca ccaccattga accgtcaaaa ttgctgttga tggtgatgga atccagcgtc
     2761 agccgtgact cccagcgact cagcgccaca tacactgccg acatgacctg caggcgtaac
     2821 gccggatttt gtggctggtc tatcagtgcc gacagcaggg aaccatattc acgacgagca
     2881 atgcggctac cctgcggcgt cagcagaatg tcccgcaccg actggcgcag atggtcaata
     2941 tcagtaatgg ctttaccgct ggtattgttc atcccgctat aaagcgtcat accgggcctc
     3001 cggttgtgtc gccgcctttc aggacgccag tatgctgatg cgcatcaacc acgatcccgt
     3061 tagaactcat cgctccgccg ccctgggtaa cgccaccatt gatcaccact tcgctgttaa
     3121 tgcgcgtgcg gtcagcctcc agtacaaact cactggtttt cagggtgatg ttgtcagcgg
     3181 cctcaatgac cattgatttg atgccactga tataccagcg cccggtggcg ggttcgtatt
     3241 caaaccagcc accgtcagga tgttctgtca cgcaggcgtc cgccgacgtc gacggtggcg
     3301 cgaactgatt cgaatagatg gcgggtaacg caaaggcggt ttccagattg ccgcccagac
     3361 tcagcagcac cacctgctca ccttccgatg gtcgccacca tgtgcgggca ttcccggcac
     3421 gcagcgtcag ccagctgatc cagttggttt caagctcgcc cgttttcacc cggcaaagcc
     3481 agttttccct gtccacttcg gtgactaccc cagtgcggat caggttggtg ataaggcgca
     3541 tgatttcggt taattgtgcg ttcataggga aaggttgcca tcaggggaag aaaggcggca
     3601 gtgctgcaac ttgtatcagt gctgatacaa agatcacccc gccagccatt gcagaatcat
     3661 gtcgcgggtc attgcctcaa catcatcatt tacacccagc aggcggcgct ctgcgtaacg
     3721 gacctccggt cctttgcgac tgacgcgatc acgcaggccg taatggtgaa cacgggcaat
     3781 acgctgcacc ttgccttcaa actgtacgct ggcagagccg gcgctggcgg tagttttcag
     3841 gtattttgtg gtgcgcagct ttgcaaacat ctgacgtttg atgcggcctt ttttgctgcg
     3901 tgctgttacc ctgcgcggtt cataactgct gccatctgga ttgcgctgca tcctgatatt
     3961 ctgctgctgt gtccggcgca gttcctgcgc cagctggcgc atcatgcggc ttctcgtgac
     4021 tggttccaga ttcgccagca aggcactcag ccagtcgtcc accttctgca gttcagccac
     4081 gtttcaccgt ccacatttct tcagattcat cgggttctgc tacagcttca acgctcgaca
     4141 cactgccgtc agtactgacc agcacacgtt ccgtcagttg caggttaagg ctgatatcac
     4201 agacatcgtt gcgcaaaata tccacctcaa aggtgaatag cttttcccgt aacgccgggt
     4261 tattgatggc atcgggctgg ttatcccgaa gccacagtaa aaccggggcc atcagcagat
     4321 tttggtcgcc gctgaaatcc tctatcacca cgttcagggt atagcggtac tcccatgaca
     4381 tggagcaggc ccccgtggca accagcgaac cgttatccac aaacagatgc agtttgtccg
     4441 ggttattgcg gacataaggc accgctttat tgagggcgtg gcgcagggat tttggtttgt
     4501 tcactgtttc gctcctgaca cgcaataatc atgtccactt tgtctgcaca gaccgcccag
     4561 gcggcctccg tttcatccag caacgcgttc aggtcaccgt tagtgcgcgg cgctgcctgc
     4621 cccagccgac acggcgtcac tcgcggacaa ccactgacgg taagctgcac ctctggtgag
     4681 tgtggggcgt tcccgcagcc ggataatatc agcaggcaaa ggagtatcag cccagcggcg
     4741 taaatcctcg ttctcacgtt tcagttcctc aatccggtgt tgtcgttgtc tcagcagtac
     4801 actggtctgt tctgcttcgg catagagccg cgcctgctcc cggttattgg cttcagtcag
     4861 aatggacagg ctgataagct ggctgttgct ctttgccagt gcctggcttt tgctccgaag
     4921 ctcgtctgcc tgcgtgctga tggtctggct ggcatcagcc agccgccacg tctgccagcc
     4981 cagcgccgcc agtaataacg ccagcacaac cagcagcaac cggttcatgc tgctacctgt
     5041 tgcgccatct gattacgggt gatccagaag gcaataacgg tcagtagata aaagacaagg
     5101 gtaatagccc accccgtcca ggcgagactg acgacaatca gcaatcgcat cacccagctg
     5161 ataaatacgt tttcttttcg ggtaattgtc ttcagcaaag atgcccttaa ctcctgccag
     5221 agcgggccat tcttaattaa cgcagccagt gctaccgtaa ttgcccccca tgtcagcaga
     5281 caggctaccc aaacaccgga cgctgccagg accggaaaaa tcccctgcgg atacaccatt
     5341 gctgcgatta acagcgccat ccataacatc agaaacagtc cgctgattaa tttctttttc
     5401 atttcagttt gctccctgta agcaccaggc catctcccgc gcacggcggt tatccagccc
     5461 ctgattaaaa acacctttta cataaaccca gcgcggcaat tgtcggcacg catccgccca
     5521 gcgccgctga ttgagtaatt tcaccagcgt ggaactgcag gcattgcccg tccccacgtt
     5581 gaaggcaaac gacaccgtag cgtcatacac cttctgcggc ggctgttgct tcacacacct
     5641 ttccagcgcc cgctccacac gtagcacgtt ggagatcagc ccttctgctg cctgtcgttc
     5701 cgtaatagtt ttgccgggaa tgacgcccga cgtattacca atgccgtcgg tccagacacc
     5761 cgcgctgcac tgatacggct gcagacgaca gccttcgtaa tcggcaatca gtttcagccc
     5821 ctccacggag gtgtgaagct gctgaaaacc cggcagcgtg gcagcaatag ccagcacggc
     5881 cccgacaagg cagcgtttaa cgattgatgg attcatagtc ctcccgcgag atctgcccgt
     5941 cgcgcagaag ctggtaggct ttgtgtttgt agtaccagtt gatagccagc atcagcacac
     6001 caatcatcag gccacccagc gttgaggcat ccttgatgga caaatcgccc agccaggcca
     6061 gcacaacggc gatgcaatac gtgataaagg cgctgattcg ctcaagcgtc ataattcagt
     6121 cccatagctg gacggtctgc acggtggtgg tggtcggaat gtccggcagc tccacctgca
     6181 gcccgtgagg taaaaaaggg ccaaattcgg caagccccgg atttgccttc agtacctgct
     6241 ccgtgacacc ctgcgtgcgc ccgtaatgac gccagcaaag cgcgtccacc gtgtcatact
     6301 gatgcgcacg cactttcatc agataagctc cactgtgcag tgcggcgcat cctgtacccg
     6361 gcagatggcc cagcgggcgt cacgccacaa atcaccgctg gcttctgcca gttcctcgcc
     6421 ccgcttcaca ccggatgccg tggcgtcata gtcctggtaa cgttcgttga gcatggcgcg
     6481 agcccagcag taaaccgcgt tgaaatagtg ctgaattcgc tcgcttttcc cgtccagctg
     6541 ttccgccgga acctctgcca gcgaggcata tcccagcatc tgctggcgtc tgcgaaactc
     6601 atacagctct gcgttgacct ccgaaattgc cgacagcgca acctgcttta aacgcggctg
     6661 cgtcaccgtg ccgtcagtgc gcatcacact gcgaaactcc gacaggtcca catcaggcca
     6721 gaacggcgta tttctgatga tttccgcctg ttccggtgcc tgttctggcg caacaaactt
     6781 catgctgctt tctcctgaaa tagagggcgg tggacggggt tttgatgtgg tagtgccttt
     6841 cgccaccccg tgccgcccgt gcgcgggggc acgttctgtc agcggctgtc attgcgcagt
     6901 ctgcgctcca gctgctgttt gtcttttttc acgccacagc ggggatcgag ctgtaacgca
     6961 tggttgagat gattaagggc ggaagccgga ttgctttcac tcaggacagc gccaatcgct
     7021 ttatgcagac gcgcccgtga ctggtccggc atatccagac cgtctgtcag ctccagcgtc
     7081 tgcagcaaca gatcggcatc aaagccggtg gcggcaagca ttgcgctctg cgcggcgtct
     7141 gccatttcct ctgccagcac ggtctgcacg ttgcggttac ccagcggcat cacccagcca
     7201 tgacgcaggg catgacgccc gatctccagc gccccggcat aatctccggc atcaatgcgc
     7261 cacagcatca cgtacatcag cacgtcatcc tgttgagcgc ctccggcagc caagacaccc
     7321 tctgcccagg cggcgtactt cggcagcagc tccaccttga tttccgcttt tttgaccgtg
     7381 gactgaacgc ccttgagacg gcggcggtct tccgccagtt gcagcagcat caggtcatag
     7441 cccgatgcgt ggcgaacact gccgccctcg cgggcggcct gttcagcctg aacgcgcagg
     7501 cgatgctgcc gtgcgggact caggctcatg gattactctc cggtttctgc tgcggcggcg
     7561 ctgaaatcgc caatctggat gttttccacc agtgcggcgc agcggtagtc ctcaaccaca
     7621 taggcttcgt taacggattc aaagttttca atccggtcac gtttcgggtt gtcgataact
     7681 gaacggcggc gggtgtcttc ctgccagtag atggacaggt tatccagacg ggtgatcagc
     7741 agcgcattcg gcgggaagaa cggcgcacgc acggcctgca ggcctcccat gcgtttctga
     7801 ctgatgatca tatcggcagc cagtttttca ctgttttcct gctctttgtt gaccagcggg
     7861 aaatacttgt cagatagcag ctcacgaccg caaatcacca ccagatcgtc atcgtcctgg
     7921 tagaccacgt cgataagctc attgacggca tccatcacca cggcgtccag gttggcatat
     7981 tcgccacctt ttccgacttt caccgcgccc ggtgtggttt caccgcccgt ggtggtgctg
     8041 cccatgacgt gatccggtgc atcctcacgg attttctgca accagccttt gttcacatcc
     8101 tgcagcagcg ggttttcgct acggttggag gttttcgcac gcttcacgcc gttaaagccg
     8161 atcatgatgc ggtccagtgc ctgacgtttc acgatggcgt cacggatacg cacctggaaa
     8221 tcctgaaact tcgcccacag gtccagcttc gcgtaggtca gtaccgtgtc aaagttggtc
     8281 tgctcgcatt tgtattccac atcgaccatc agcgtaggat cgacaggttc acgctctttc
     8341 gcggtggtgt cagtggttcc ggcaatggtg ctaccaacac ccaaccccag cagctgaccg
     8401 gactgctcag tcactggcgt gacgttaatc agcgtcagga aagcggcgga ctgctggatc
     8461 tggtcttcca gcgtctgctg tacagacggc tccacggtga acttgctgga cagttcttca
     8521 actgccacac cgttcagacg cgccagctgc tgcaggtaag cgttaaaagc aaagcgggta
     8581 ttcttcttca tcaggttttg tgctccatca gcaattggtc agagtgtcag cgggggcgtt
     8641 accgcctgtt gcacgctggc ggtagtcctg gcggctgtct tcatgactca gcttattcac
     8701 cagttcgtta aaggcggttt gctgctcctg cagagcagtc tccagttcag acaggcgttc
     8761 ttcctgctca gacagggatt tttcggtgcg tgcgctcagg ttctgctgct cagtggcaac
     8821 cagctccacg gccttatgca catcagagaa ccgggcgtca tcggactgtt cttttttggt
     8881 aaacaacgcc gtgacacggg caaacaggga cggtttgtca tcctggattt cttccagttc
     8941 gatcaccgtt tcctctgcag cggtaaagag atttgcagga ttctgcttgc ggtttgccag
     9001 cgggttatga gctgcactgg cgctgaatgt cagcatttcc gtacccagac tggcagggtc
     9061 atcagtggca gccaggccga ccaggtaggc tttgcccgta tcagcgaact tcgggctgac
     9121 ttccatagag gtgaataatt tctggccttt tttcaccagt tccaccaggg actccgttgg
     9181 ctcaacatcg gcatacagcg ccatcttgcc tgccagcgga ccttccgtta tttcttcagc
     9241 aaacagcgcc gtcaccttgc cgtagcggtt aaaggtgctg tccggcagat aagacttgat
     9301 gtgctcaagg ttaatcagcg cggtatacac cgccgggttg tagctggctg ccatctgttc
     9361 cagccattca cgctggattt cgcgtccgtc ggtggtggca ccttccaccc cgatgcgaaa
     9421 acgctttgct ttcactgtca tgagccgtgc tccgttagaa aaaacttact ggagccttat
     9481 ggttgcggtg atgggggcag tgaaacaatg cgcggtattt gtaccgacaa ccacacaaac
     9541 cgcaggcggg gaaagccttc attcaaggct gtaggtttgt gccatgaaca ccacactgac
     9601 acccgcagat ctcgatcccc gtcggcaggc catgctgctg tactttcagg gataccgcgt
     9661 cgcccgcatt gctgaaatgc tgggcgagaa agttgcaacc gttcacagct ggaaaaaacg
     9721 cgacaagtgg ggtgactatg ggccgctgga tcagatgcag ctcaccaccg ccgcacgcta
     9781 ctgccagctc attatgaagg agcacaaaga agggaaagat ttcaaagaaa ttgacctgct
     9841 ggcacgccag tctgagcgcc acgcgcggat cggcaagttt aacaatggcg gcaacgaagc
     9901 cgacttaaac cctaacgttg ccaaccgcaa caaaggccca cgtcgtcagc cggaaaagaa
     9961 tgttttcacc gatgaacaga ttgagaaact ggaagaaatc ttccattcct ctatgttcaa
    10021 ctaccagcgc cactggtggg aagccggaaa aaccaaccgc atccgcaacc tgctgaagtc
    10081 acgccagatc ggcgcgacct tttactttgc ccgtgaagcc ctgattgacg ccctgctgac
    10141 cgggcgtaac cagattttcc tttctgccag caaggctcag gcccacgtct ttaaacagta
    10201 catcatcgac tttgccaaag aagtggaggt ggagctgaaa ggcgatccga tggtgcttcc
    10261 taacggggcc acgctttact tcctcggcac caatgcccgc acggcccaga gttaccacgg
    10321 caacctgtat ctggatgaat atttctggat accgaaattc caggagctgc gcaaagtggc
    10381 ttccggtatg gctattcaca aaaaatggcg acaaacctat ttttccacgc catccagtct
    10441 gacacacagt gcttatccgt tctggtccgg tgcgctgttc aaccgtgggc gcaacaaagc
    10501 cgataaggtg gacatcgacc tgtcccacag caatctggcc cccggcctgc tgtgcgcaga
    10561 cgggcaatac cgccagatag tcaccgtgga agatgcggtg cgcggcggct gtaacctgtt
    10621 cgacctcgac cagctacgca tggagtacag cccggacgaa taccagaacc tgctgatgtg
    10681 cgagtttgtg gacgatctcg catccgtgtt tccgctcagc gagctgcagg cgtgcatggt
    10741 ggacagttgg gaagtctgga ccgactttca tgcactggcc ctgcgcccgt ttggctggcg
    10801 cgaggtgtgg atcggttatg acccggcaaa aggtacgcag aatggcgaca gcgccggatg
    10861 cgtggtggtg gcaccgccag ccgtgccagg cggtaagttt cgcattcttg agcgtcacca
    10921 gtggcgcggg atggacttcc gcgcccaggc tgatgcaatc aaaaaactga ccgagcagta
    10981 caacgtgacc tatatcggca tcgactcgac aggcgtcggt cacggggttt atgagaacgt
    11041 gaaagcgttc tttcctgccg tccgggagtt tgtctacaac cccaacgtta aaaacgccct
    11101 ggtactcaag gcctacgaca ttatcagcca ccgccgtctg gagtttgacg ccgggcacac
    11161 cgacattgcg cagtcattca tggcaatccg tcgcgccacc accgccagcg gcaaccgccc
    11221 gacctatgaa gctagccgca gcgaagaagc cagccacgcc gatctggcct gggcaacaat
    11281 gcacgcactg tttaacgaac cgctgcaggg cgagtccgcc aataccagca atattgtgga
    11341 gattttttga tgggaaagag taagaaaaac cgcgctgctg cgacgaaacg gagccagcat
    11401 aaaaatcaaa cttcagccga agctttcagc tttggtgatc ccattcctgt actggaccgc
    11461 cgcgaactgc tggactatgt ggaatgcgta cagacggatc gctggtatga gccacccgta
    11521 agttttgacg gactggcgcg caccttccgg gctgccgtgc atcacagttc cccgattgca
    11581 gtaaagtgca acattctgac cagtacctat atccctcatc cgctgctcag ccaacaggct
    11641 ttttcgcgtt ttgtgcagga ctatctggta tttggtaacg cctacctgga gaaacgcacg
    11701 aaccgattcg gtgaagttat agcccttgag cctgcgctgg caaaatacac ccgacgcggg
    11761 ttagacctgg atacctactg gtttgtgcaa tacggtatga caacccagcc gtatcagttc
    11821 acgaaaggca gcatttttca tctgatggaa cctgacatca accaggagat ctacggcctg
    11881 ccaggttatc tttctgccat tccgtcagcc ctgctcaacg agtccgccac gctgttccgc
    11941 cggaagtatt acattaacgg cagtcatgca ggcttcatca tgtacatgac cgatgccgcg
    12001 cagaaccagg aggatgtgaa caacctccgc aatgcgatga aaagcgccaa aggccctggc
    12061 aacttccgca acctgtttat gtactcgcct aacggcaaaa aggacgggct tcagatcatc
    12121 ccgttgtcag aagtcgcggc gaaggatgag tttctgaata tcaagaacgt gagccgggat
    12181 gacatgatgg ctgcgcatcg tgtgccgcca caaatgatgg ggataatgcc aaataatgtc
    12241 gggggatttg gggatgtgga gaaagccagc agggtctttg tccgtaatga gctaattcca
    12301 ttgcaaaaaa ggcttcaaga agtaaatgaa tggataaagg agccaataat gcaatttaat
    12361 gaatataacc ttgcatagga atatgaaaag agccgctaaa gcggctcaat aaactcactc
    12421 gggaataccc ttcctttcag ggttaaccaa ccagtgcttt atattcccaa taaaatccca
    12481 aacatcacct tcagatggct tcacatatga ttcatcatta ggatcatccc ccatcttctc
    12541 gaaaatactt gttaaccaag tattctttcc tcgacctatt tcaagatttt gcaaccaaga
    12601 ctcgatctcg cccgttggaa cgacaaaaac cccataatct gaaagggatt taaaaaactc
    12661 agagcaggcg gttttatctg aacccgataa caaggagagg cccccatctg tcttcatatt
    12721 tttattcgta tttttgaagg cttgaaaaag cttttgtctt tccaaaccta aagtttcatg
    12781 cttcatttct ggaaaaaaag ctccaagcat tggttttttc catacaactc ctccctcctt
    12841 caatatatcg atatctacca ctccaacgca gggaatacct agctcacgaa ggggttttac
    12901 tatatcccaa acagtttgtt tgttttgagc attcaaaaac aggcatccct caataccccg
    12961 aggatctcca gcagccaata agcgctcatt aatctcttga taaaatgccc tatctgcatc
    13021 cgcttcagta acgacaacgt aattataaaa caatgcgttt agcacaccaa tagatcttaa
    13081 taacggattt ctcatgagcg gcgtcaattg ttcctttgtt agtaatctag acgtaccaga
    13141 tttaccatca aaagtaagcc taactatatt caatggcact ttaccttgaa cacaacccat
    13201 taaaaaatta gcgctatgcg ttgaaataaa cacattttta ttactatgag cactaatcct
    13261 agatatttcc tttcctaact ttgagcataa ggatggatgt aaaaaagctt ccggctcatc
    13321 aattagagtc acctttggtg agccaactat cagcgaaatc atcatcccaa caaaagcatt
    13381 aacaccatcg ctagcctctg taatcaatga cgcttttgaa tgaaaaagca ctgacttttc
    13441 atcccaacct ctttcttctt gttcagtctc aggagcgcgc tcagaaagac gaattctaag
    13501 ataacctata tttgttggat caataacaag atacttcccg aaagcatcaa aaacgatatc
    13561 tcttagctcc atccttgctt tattattttt aaaaagaaat gaaaggttat tcgtaggttc
    13621 tccttgtaaa tcggatgaac cttgacttga agttaaggaa agtcgacttc taccatccaa
    13681 ccttagagtc agcaacgagt aatattgata tagatgctcc cgattgaatt cattgggttg
    13741 ctggaattct cttaacattc catccaaata cactagaatc ctagatattc cttcaccacg
    13801 gaggtttagc ctagataaaa caacatgccc cgcatgcttt tcatcctggt ttggctcaac
    13861 ttgtaaagat tcaatttcct tttttgcttc atcctcagaa agtggtttga ttaatatctg
    13921 ttttattact tctccctgcg ctgcatttcg ggttgtcagt tgattttcta tatcgattaa
    13981 agctctagat ttaccactat tattcggccc cacaaaaatt gtca
//
LOCUS       DBANUU010000116        13931 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000116 DBANUU010000000
VERSION     DBANUU010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.117"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(101..1459)
                     /gene="plaP"
                     /locus_tag="ACIE68_003522"
     CDS             complement(101..1459)
                     /gene="plaP"
                     /locus_tag="ACIE68_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFX5909782.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1449..1511)
                     /gene="yoeI"
                     /locus_tag="ACIE68_003523"
     CDS             complement(1449..1511)
                     /gene="yoeI"
                     /locus_tag="ACIE68_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFX5909783.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1726..2655)
                     /gene="yeeY"
                     /locus_tag="ACIE68_003524"
     CDS             complement(1726..2655)
                     /gene="yeeY"
                     /locus_tag="ACIE68_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFX5909784.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2701..3525)
                     /gene="yeeZ"
                     /locus_tag="ACIE68_003525"
     CDS             complement(2701..3525)
                     /gene="yeeZ"
                     /locus_tag="ACIE68_003525"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFX5909785.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3608..3862)
                     /gene="yoeB"
                     /locus_tag="ACIE68_003526"
     CDS             complement(3608..3862)
                     /gene="yoeB"
                     /locus_tag="ACIE68_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFX5909786.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3859..4110)
                     /gene="yefM"
                     /locus_tag="ACIE68_003527"
     CDS             complement(3859..4110)
                     /gene="yefM"
                     /locus_tag="ACIE68_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFX5909787.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4393..4443
                     /gene="hisL"
                     /locus_tag="ACIE68_003528"
     CDS             4393..4443
                     /gene="hisL"
                     /locus_tag="ACIE68_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFX5909788.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    4419..4542
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4589..5488
                     /gene="hisG"
                     /locus_tag="ACIE68_003529"
     CDS             4589..5488
                     /gene="hisG"
                     /locus_tag="ACIE68_003529"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFX5909789.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5494..6798
                     /gene="hisD"
                     /locus_tag="ACIE68_003530"
     CDS             5494..6798
                     /gene="hisD"
                     /locus_tag="ACIE68_003530"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFX5909790.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTSPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLASAERLTAHKNAVTLRVNALKEQA"
     gene            6795..7865
                     /gene="hisC"
                     /locus_tag="ACIE68_003531"
     CDS             6795..7865
                     /gene="hisC"
                     /locus_tag="ACIE68_003531"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFX5909791.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7865..8932
                     /gene="hisB"
                     /locus_tag="ACIE68_003532"
     CDS             7865..8932
                     /gene="hisB"
                     /locus_tag="ACIE68_003532"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFX5909792.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQ
                     KAGYKLVMITNQDGLGTQSFPQVDFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8932..9522
                     /gene="hisH"
                     /locus_tag="ACIE68_003533"
     CDS             8932..9522
                     /gene="hisH"
                     /locus_tag="ACIE68_003533"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFX5909793.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKASRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9522..10259
                     /gene="hisA"
                     /locus_tag="ACIE68_003534"
     CDS             9522..10259
                     /gene="hisA"
                     /locus_tag="ACIE68_003534"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFX5909794.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSQDMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10241..11017
                     /gene="hisF"
                     /locus_tag="ACIE68_003535"
     CDS             10241..11017
                     /gene="hisF"
                     /locus_tag="ACIE68_003535"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFX5909795.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLDDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            11011..11619
                     /gene="hisIE"
                     /locus_tag="ACIE68_003536"
     CDS             11011..11619
                     /gene="hisIE"
                     /locus_tag="ACIE68_003536"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFX5909796.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGETAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQGLDLGEVIDNLKNRH"
     gene            complement(11658..12773)
                     /gene="wbdC"
                     /locus_tag="ACIE68_003537"
     CDS             complement(11658..12773)
                     /gene="wbdC"
                     /locus_tag="ACIE68_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004184789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 3-alpha-mannosyltransferase WbdC"
                     /protein_id="HFX5909797.1"
                     /translation="MRVLHVYKTYYPDTYGGIEQVIYQLSQGCARRGIAADVFTFSPD
                     KDTGPVAYEDHRVIYNKQLFEIASTPFSLKALKRFKLIKDDYDIINYHFPFPFMDMLH
                     LSARPDARTVVTYHSDIVKQKRLMKLYQPLQERFLSGVDCIVASSPNYVASSQTLKKY
                     LDKTVVIPFGLEQQDVQHDPQRVAHWRETVGDKFFLFVGTFRYYKGLHILMDAAERSR
                     LPVVVVGGGPLESEVRREAQQRGLSNVMFTGMLNDEDKYILFQLCRGVVFPSHLRSEA
                     FGITLLEGARFARPLISCEIGTGTSFINQDKVSGCVIPPNDSQALVEAMNELWNNEET
                     SNRYGENSRRRFEEMFTADHMIDAYVNLYTTLLESKS"
     gene            complement(12783..13928)
                     /locus_tag="ACIE68_003538"
     CDS             complement(12783..13928)
                     /locus_tag="ACIE68_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFX5909798.1"
                     /translation="MKIIFATEPIKYPLTGIGRYSLELVKRLAVAREIEELKLFHGAS
                     FIEQIPLVENKSDTKASNHGRLSAFLRRQTLLIEAYRLLHPRRQAWALRDYKDYIYHG
                     PNFYLPHKLERAVTTFHDISIFTCPEYHPKDRVRYMEKSLHESLDSAKLILTVSDFSR
                     SEIIRLFNYPEERIVTTKLACSSDYIPRSPAECLPVLQKYQLAWQAYALYIGTMEPRK
                     NIRGLLHAYQLLPMEIRMRYPLILSGYRGWEDDVLWQLVELGTREGWIRYLGYVPDED
                     LPYLYAAARVFVYPSFYEGFGLPILEAMSCGVPVVCSNVTSLPEVVGDAGLVADPNDI
                     DAISAQILQSLQDDSWREIATARGLAQAKQFSWENCATQTINAYKLL"
BASE COUNT         3478 a         3680 c         3627 g         3146 t
ORIGIN      
        1 aattagaaca aatggttatt aatgaggatg tcgataaaaa agaataaaaa aaccggagcc
       61 aaatattcat tcgactccgg tttattatta caaaagatga ttacgctacg tcttcgtact
      121 gcggaaccgg gttacggaaa ctcttggtga cgcaagccag gtaaatcaga ccgatagccg
      181 cccagatcag acccagaacc attgagcttt cttccaggtt aacccacagc gcaccaacgg
      241 tcagcgcacc acacatcggc aggaacagat actggaagtg atctttcagc gtcttgttac
      301 gcttctcacg gatccagaac tgcgagatga ccgacaggtt aacgaaggtg aacgccacca
      361 gcgcaccaaa gttaatcagc gccgtagcca ttaccaggtc gaagttgatt gccagcaggg
      421 caatcgcgcc aaccaggatg atgttcatcg ccggagtacg ccatttcggg tgtacataac
      481 cgaagaagct tttcgggaac acaccgtcac gccccattac gtacatcaga cgcgctacgc
      541 ctgcatgcgc cgccataccg gacgccagta cggtaatggt ggagaagatc agcgcgccaa
      601 cctggaacgc tttacccgca acgtacagca tgatttcagg ctgtgacgca tccggatctt
      661 taaagcgaga gatatccggg aagtacagct gcaggaagta agttgcaaag atgaagatca
      721 tgccgccaat cagtgcggtc aggaaaatcg cacgcgggat cacgcgctct gcatctttgg
      781 tttcttccga caggttgctg atgccgtcaa agccggtaaa ggagaagcac aggattgtcg
      841 ccccggtaat catcgggata acatgcgcat cgccagacca gaacggacga gtgctcgcca
      901 gcgtacccgc gccttcacct tcaaatacgc cataaacaac catgcccaga atcaccgcga
      961 tcagcactac ctgcaacacg acgattacag tgttgaagtt cgctacggat ttcagactac
     1021 gcaggttaaa ggcggtcata aaggccacca gcgccaccac aaacatccac gatgggatgg
     1081 aaggcaccag agcttcaaaa tagattttcg ccagcagaat gttgatcatc ggcgcgaaca
     1141 gatagtcgag cagagaagac caacccacca taaagccgac agtcgggcta atggatttct
     1201 gggcgtaagt gtatgcagag ccagcagaag gatagcggcg aaccagcttc ccgtagctca
     1261 gagccgtaaa caggatcgca atcaatgcga acgcataggc tgtcggcaca tgaccatccg
     1321 tgaggcctga aacgatacca aatgtatcaa acagcgtcat cggctgcata taggcaagac
     1381 ccatcattac aaccggaact aacgtaagcg ttttacgcaa ttccacgcga gaggtgtttg
     1441 gagtaacgtt atgcgacatg gtcattctcc tttacggtga taaccgtcgc gtaagcaaaa
     1501 aattgcccca tttttttgga ttcctcagcg acaacaactg tcgattttta gtaaatatct
     1561 atccggtacg aagcccggcc tcttggtatg aatgattggt ttgaagcaaa aaataaccga
     1621 cgctaattaa acgtcggttt ttagtcattt tttgacagcg gcgcattgtg cctaaatggg
     1681 ggggaaatga caagagaatg agaggcttgt cataataatt tttctttaaa tggctgattt
     1741 tccgtcatca gattttgcat aaacaccggc gatcgcacta tttgctaaaa tttcgtcccg
     1801 ccaccaacca ctggcaagcc ccgcggtctg ttcattccag ccaatccaga cgggttcaaa
     1861 gagggtttgg gcgaccactt tcttctcgat taacgcacca ctatcgagaa aacgttgcgc
     1921 cagataacgg ggtaaatagc cgcagccgag gccgctaatt tgcagctcca gcttggtttt
     1981 aaaatcgaaa acggtaatag cttcttgttc gtcgagaagt tgtgaggccg tagaagcgcc
     2041 ggcctgtgca gtgtccccca ccacaatcgc ccgatatcgt ttaatgatac gacggtttaa
     2101 cggttcttct tccagggcta acggatgatg aggcgcgacc gcaaagactt gttccagatc
     2161 gcccaaccga gagaaaccaa actcactgga agatggtggc tcatgcatcg cgccaacaat
     2221 aatatccgct cgcccctgag tcagggcatc ccatgaacca ccgagtacac cattgataaa
     2281 cttcaggcgc gtcacgctat gatgttgata aaaagcctca atgagtggcg caagcagaga
     2341 aaaagggaag gtgtcgtcca cgccaatcac cagctcgttt tcccagcctt catgcagttt
     2401 gatcgcctgc ttttccagtt ctcgcacggt atgcagaact tcccgccctt tctctaataa
     2461 catttttccg gtgcgggtga atttagcgcg gtgaccgcta cgatcaagca attgaatatt
     2521 aaggtcgctt tccagcttgt gaacggtata actcagagcg gacggcgtct tgtagagttt
     2581 agccgacgcc gccgcaaaac tgccttcttt ttctaaggca tcgaggatca tcaacacatc
     2641 cagcagtggt ttcatacacg cccccttgca gtgtatgcgg cgtcctgctg gcgtgatcgt
     2701 ttactccagc ggcattacca gcggatcggg atactggtat tcaaatccca gttcattaca
     2761 aatccgactg ccatcaataa tcttaccttt gccgctgtcc agactatttc tgaactgcgg
     2821 aggttccagc cccaataaac gggccatctg cggatagaaa acattacgcg cagggtgagc
     2881 tggcgcacat atattataga tgtgtccgcc tttaggtgcc tgtaacaaca gagtgatagc
     2941 gccaataaca tcttctaaat ggactaaatt aacaccatgt tcaccatcag gggcggtttt
     3001 tccggcaaag aagcgtccgg gatgacgtcc cggtccgacc aggcccgcaa gacgcagaat
     3061 atcgaccgaa gtaccgggta aattgtgcag ccagtcttcg agttcttcta atactcgtcc
     3121 actgttggtc accggattac gcggggtggt ttctttcacc gtgccttgcg cgtcgccata
     3181 gacagatgtc gagctggtaa aaataatgcg gggaatacga tgggccagcg cgctatccac
     3241 taactcttgt accgcttgta aatagaactc atcgccgggg ccgctacgac gtgccggaag
     3301 cgtaatgacc agcgcatcgg catccatcag ggcatccaga tcgtcagaat cgcaaactaa
     3361 ctcaggctcc atgcgaagca gatagctatc aatgccactc attcgggccg cttcgacacc
     3421 atcttgtgtg gttttactcc cggtgacttg ccagcctcgc gctgaaagtg acatcgccag
     3481 cggcatgcct aaccacccta acccgacaat tgcgaccttt ttcatccgtt atctcctgat
     3541 acaccttaga tctataaggc tacgctagcg tatcaaaact gacaattcat tctatgaatg
     3601 aatctgttca ataatgataa cgacatgctg caatgagcag tgaatcatca gtaaccgcgt
     3661 ataccagacg gtgctcctct gtaatgcgtc gggaccagaa acctgacaaa ttatgtttca
     3721 ggggttctgg tttcccctta ccttcaaatg gtgttctgcg ggtatctttg ataagttcat
     3781 tgatcttttt aacaattcgc ttatctgttt cctgccagta cagataatca tcccatgatt
     3841 cctcagacca gattagtttc actcaataat gtccttttcc gttcctttgc ctgatttcag
     3901 gctatcgatt gagtccatca atctccgggc gttagcgggg gaacgcagta gataagccgt
     3961 ctcttccagc gagttgtatt cttcgagtga catcagaaca caagcctctc cattctgacg
     4021 agtaatgagg atcggggcat gatcttcaac ggctttcatc attgttgccg acaaattctg
     4081 acgcgcttcg ctgtagctaa ttgtacgcat gtaaatctcc tcttttgtac agttcattgt
     4141 acaatgatgg gtgttaattc actatttatt aattagtttg tagatcaaga cattgccagc
     4201 gagacgaaaa atcagacttt gctctttttg gtgctgtcaa gttagaggac agtcctctta
     4261 gccccctcct tttcccgctc attcattaaa caaatccatc gccataaaat atataaaaaa
     4321 gcccttgctt tctaacatga aagtggttta ggttaaaaga catcagttga ataaacattc
     4381 acagagactt ttatgacacg cgttcaattt aaacaccacc atcatcacca tcatcctgac
     4441 tagtctttca ggcgatgtgt gctggaagac attcagatct tccagtggtg catgaacgca
     4501 tgagaaagcc cccggaagat caccttccgg gggctttttt attgcgcgat tgataacggt
     4561 tcagacaggt ttaaagagga ataagaaaat gacagacaac actcgtttac gcatagctat
     4621 gcagaaatcc ggccgtttaa gtgatgactc acgtgaattg ctggcgcgct gtggcattaa
     4681 aatcaatctt cacacccagc gcctgatcgc gatggcagaa aacatgccga ttgatattct
     4741 gcgcgtgcgt gacgacgaca ttcccggtct ggtaatggac ggcgtggttg acctcgggat
     4801 tatcggcgaa aacgtgctgg aagaagagct gcttaaccgc cgcgcccagg gtgaagatcc
     4861 acgctacttt accctgcgtc gtctggattt cggcggctgc cgtctttcgc tggcaacgcc
     4921 ggttgatgaa gcctgggatg gcccgctctc cttaaacggt aaacgtatcg ccacctctta
     4981 tcctcacctg ctcaagcgtt atctcgacca gaaaggcatc tcttttaaat cctgcttact
     5041 gaacggttct gttgaagtcg ccccgcgtgc cggactggcg gatgcgattt gcgatctggt
     5101 ttccaccggt gccacgctgg aagcaaacgg cctgcgcgaa gtcgaagtta tctaccgctc
     5161 gaaagcctgc ctgatccagc gcgatggcga aatggaagaa tccaaacagc aactgatcga
     5221 caaactgctg acccgtattc agggtgtgat ccaggcgcgc gaatcaaaat acatcatgat
     5281 gcacgcaccg accgaacgtc tggatgaagt catcgccctg ctgccaggtg ccgaacgccc
     5341 aaccattctg ccgctggcgg gtgaccaaca acgcgtagcg atgcacatgg tcagtagcga
     5401 aaccctgttc tgggaaacga tggaaaaact gaaagcgctg ggtgccagtt caatcctggt
     5461 cctgccgatt gagaagatga tggagtgatc gccatgagct ttaacacaat cattgactgg
     5521 aatagctgta ctgcggagca acaacgccag ttgttaatgc gcccggcgat ctccgcttct
     5581 gaaagcatta cccgcaccgt taacgatatt ctcgataacg tgaaaacgcg tggcgatgaa
     5641 gccctgcgtg aatacagcgc gaagtttgat aaaaccacgg ttaccgcgct gaaggtgtct
     5701 gcagaggaaa ttgccgccgc cagcgaacgc ctgagcgacg agctaaaaca ggcgatggcg
     5761 gtggcagtaa agaatattga aaccttccac actgcgcaaa aactgccgcc ggtagatgta
     5821 gaaactcagc caggcgtacg ttgtcagcaa gtcacgagcc cggtggcttc ggtaggtttg
     5881 tatattcctg gcggctccgc cccgctcttc tcaacggtat taatgctggc aactccggcg
     5941 cgtattgcgg gctgtaaaaa agtggtgctg tgctcaccgc cgccgattgc cgatgagatc
     6001 ctttatgcgg cgcagctgtg cggtgtacag gacgtgttta acgtcggcgg cgcacaggcc
     6061 attgccgcac tggcgtttgg tactgaatct gtgccaaaag tggacaaaat cttcgggcca
     6121 ggcaacgcct ttgtcaccga ggcaaaacgc caggtaagcc agcgtctgga cggtgcagcg
     6181 atcgatatgc ccgcaggccc gtcggaagtg ctggtgattg ccgacagcgg cgctacgcca
     6241 gatttcgtgg cttctgattt gctttctcag gctgaacacg gcccggactc tcaggtgatt
     6301 ttactgacgc ccgatgctga tatggcgcgt cgcgttgccg aagctgtcga acgccagctg
     6361 gcagaactgc cgcgtgccga aaccgcacgt caggcactga gcgccagccg cctgatcgtg
     6421 actaaagatt tagcgcagtg cgtagagatt tccaaccagt acggcccgga gcacctgatc
     6481 attcagaccc gcaacgcccg cgaactggtc gatggcatca ccagcgccgg ttcggtattt
     6541 cttggtgact ggtcaccgga atcggcaggc gactatgcct ccggcactaa ccatgttcta
     6601 ccgacttacg gttacaccgc cacctgttcc agcctcgggc tggcagattt ccagaagcgc
     6661 atgaccgtgc aggaactctc gaaagaaggc ttttccgcgc tggcttcgac cattgaaaca
     6721 ctggcctccg ccgagcgcct gaccgcccat aaaaatgccg ttactttgcg tgttaacgcc
     6781 cttaaggagc aagcatgagc accgtgacta ttaccgattt agcgcgtgaa aacgtccgca
     6841 acctgacgcc gtatcagtca gcgcgtcgtc tgggcggtaa cggcgatgtc tggctgaacg
     6901 ccaacgaata ccccactgcc gtggagtttc agcttactca gcaaacgctc aaccgctacc
     6961 cggaatgcca gccgaaagcg gtgattgaaa attacgcgca atatgcaggc gtaaaaccgg
     7021 agcaggtgct ggtcagccgt ggcgcggacg aaggtattga actgctgatt cgcgcttttt
     7081 gcgaaccggg taaagacgcc atcctctact gcccgccaac gtacggcatg tacagcgtca
     7141 gcgccgaaac gattggcgtc gagtgccgca cagtgccgac gctggacaac tggcaactgg
     7201 acttacaggg catttccgac aagctggacg gcgtaaaagt ggtctatgtt tgcagcccca
     7261 acaacccgac cgggcaactg atcaacccgc aggattttcg caccctgctg gagttaacgc
     7321 gcggtaaggc gattgtggtt gccgatgaag cctatataga gttttgtcca caggcatcgc
     7381 tggctggctg gctggcggaa tatccgcacc tggctatttt gcgcacactg tcgaaagctt
     7441 ttgctctggc ggggcttcgt tgcggattta cgctggcaaa cgaagaggtc atcaacctgt
     7501 taatgaaagt gatcgccccc tacccgctct cgacgccggt tgccgacatt gcggcccagg
     7561 cgttaagccc gcaggggatc gtcgccatgc gtgagcgggt ggcgcaaatt attgcagaac
     7621 gcgaatatct gattgccgca ttgaaagaga tcccctgcgt ggagcaggtt tttgactctg
     7681 aaaccaacta cattctggcg cgctttaaag cctccagcgc agtgtttaaa tctttgtggg
     7741 atcagggcat tatcttacgt gatcagaata aacaaccctc tttaagcggc tgcctgcgaa
     7801 ttaccgtcgg aacccgtgaa gaaagccagc gcgtcattga cgccttacgt gcggagcaag
     7861 tttgatgagt cagaagtatc tttttatcga tcgcgatgga accctgatta gcgaaccgcc
     7921 gagtgatttt caggtggacc gttttgacaa actcgccttt gaaccgggcg tgatcccgca
     7981 gctgctgaag ctgcaaaaag cgggctacaa gctggtgatg atcaccaatc aggatggtct
     8041 gggaacacaa agtttcccgc aggtggattt cgatggcccg cacaacctga tgatgcagat
     8101 cttcacctcg caaggcgtgc agtttgatga agtgctgatt tgtccgcacc tgcccgccga
     8161 tgagtgcgac tgccgtaagc cgaaagtaaa actggtggag cgttatctgg ctgagcaagc
     8221 gatggatcgt gccaatagtt atgtgattgg cgatcgcgcg accgatattc agctggcgga
     8281 aaacatgggc attaatggtt tacgctacga ccgcgaaacc ctgaactggc caatgattgg
     8341 cgagcagctc actaaacgag accgttacgc ccatgtagtg cgcaacacca aagagacgca
     8401 aattgacgtc caggtgtggc tggatcgcga aggtggcagc aagattaaca ccggcgttgg
     8461 cttctttgat catatgctgg atcagatcgc cacccacggc ggtttccgta tggaaatcaa
     8521 cgtcaaaggc gacctctata tcgacgatca ccacaccgtc gaagataccg gcctggcgct
     8581 gggcgaagcg ttaaaaatcg cccttggcga caaacgcggt atttgccgct ttggttttgt
     8641 actgccgatg gacgaatgcc ttgcccgctg cgcgctggat atctctggtc gcccgcacct
     8701 ggaatataaa gctgaattta cctaccagcg cgtgggcgat ctcagcaccg agatgatcga
     8761 gcacttcttc cgttcactct cttacaccat gggcgtgacg ttgcacctga aaactaaagg
     8821 taaaaacgat caccaccgtg tagagagcct gttcaaagcc tttggtcgca ccctgcgcca
     8881 ggccatccgc gtggaaggcg ataccctgcc ctcgtcgaaa ggagtgctgt aatgaacgtg
     8941 gtgatccttg ataccggctg cgccaacctg aactcggtga agtctgccat tgcgcgtcac
     9001 ggttatgaac ccaaagccag ccgtgacccg gacgtcgtgt tgctggccga taaactgttt
     9061 ttacccggcg ttggcactgc gcaagcggcg atggatcagg tacgtgagcg cgagctgttt
     9121 gatctcatca aagcctgtac ccaaccggtg ctgggcatct gcttagggat gcaactgctg
     9181 gggcggcgca gcgaagagag caacggcgtc gacttgctgg gcatcatcga cgaagacgtg
     9241 ccgaaaatga ccgactttgg tctgccactg ccacatatgg gctggaaccg cgtttacccg
     9301 caggcaggca accgcctgtt tcaggggatt gaagacggcg cgtactttta ctttgttcac
     9361 agctacgcaa tgccggtcaa tccgtggacc atcgcccagt gtaattacgg cgaaccgttc
     9421 accgcggcgg tacaaaaaga taacttctac ggcgtgcagt tccacccgga gcgttctggt
     9481 gccgctggtg ctaagttgct gaaaaacttc ctggagatgt gatgattatt ccggcattag
     9541 atttaatcga cggcactgtg gtgcgtctcc atcagggcga ttacggcaaa cagcgcgatt
     9601 acggtaacga cccgctgccg cgattgcagg attacgccgc gcagggtgcc gaagtgctgc
     9661 acctggtgga tctgaccggg gcaaaagatc cggctaaacg tcaaatcccg ctgattaaaa
     9721 ccctggtcgc gggcgttaac gttccggtgc aggttggcgg cggcgtgcgt agcgaagaag
     9781 atgtggcggc gttactggaa gcgggcgttg cgcgcgtagt ggtcggctcc accgcggtga
     9841 aatcacaaga tatggtgaaa ggctggtttg aacgcttcgg tgccgatgcc ttagtgctgg
     9901 cgctggatgt ccgtattgac gagcaaggca acaagcaggt ggcagtcagc ggctggcaag
     9961 agaactcggg cgtttcactg gaacaactgg tggaaaccta tctgcccgtc ggcctgaaac
    10021 atgtgctgtg taccgatatc tcgcgcgacg gcacgctggc aggctctaac gtctctttat
    10081 atgaagaagt gtgcgccaga tatccgcagg tggcatttca gtcctccggc ggtattggcg
    10141 acattgatga tgtggcggcc ctgcgtggca ctggtgtgcg cggcgtaata gttggtcggg
    10201 cattactgga aggtaaattc accgtgaagg aggccatcgc atgctggcaa aacgcataat
    10261 cccatgtctc gacgttcgtg atggtcaggt ggtgaaaggc gtacagtttc gcaaccatga
    10321 aatcattggc gatatcgtgc cgctggcaaa acgctacgct gaagaaggcg ctgacgaact
    10381 ggtgttctac gatatcaccg cttccagcga tggccgtgtg gtagataaaa gctgggtatc
    10441 tcgcgtggcg gaagtgatcg acattccgtt ctgtgtggcg ggtgggatta aatcgctgga
    10501 tgacgccgcc aaaattcttt cctttggcgc ggataaaatc tccattaact cccctgcact
    10561 ggcggacccg acgttaatta ctcgcctggc ggatcgcttt ggcgtgcagt gtattgtggt
    10621 cggtattgat acctggtacg acggcgaaac cggtaaatat catgtgaatc aatataccgg
    10681 cgacgaaagc cgcacccgcg tcactcagtg ggaaacgctc gactgggtac aggaagtgca
    10741 aaaacgcggt gccggagaaa tcgtcctcaa catgatgaat caggacggcg tgcgtaacgg
    10801 ttacgacctc gaacaactga aaaaagtgcg tgaagtttgc cacgtcccgc taattgcctc
    10861 cggtggcgcg ggcaccatgg aacacttcct cgaagctttc cgcgatgccg acgttgacgg
    10921 tgcgctggca gcttccgtat tccacaaaca aataatcaat attggtgaat taaaagcgta
    10981 cctggcaaca cagggcgtgg agatcaggat atgttaacag aacaacaacg ccgcgaactg
    11041 gactgggaaa aaaccgacgg acttatgccg gtgattgtgc aacacgcggt atccggcgaa
    11101 gtgttaatgc tgggctatat gaacccggaa gccttagaca aaaccatcga aagcggcaaa
    11161 gtcactttct tctcgcgcac taaacagcga ctgtggacca aaggcgaaac gtcgggcaat
    11221 ttcctcaacg tagtaaatat tgcgccggac tgcgacaacg acacgctact ggtactggcg
    11281 aatcctatcg gaccaacctg ccataaaggc accagcagct gcttcggcga aactgctcac
    11341 cagtggctgt ttctgtatca actggaacaa ctgctcgccg aacgcaaatc agccgacccg
    11401 gaaacctcct acaccgccaa actgtatgcc agcggcacca aacgcattgc gcagaaagtg
    11461 ggtgaagaag gggtggaaac cgcactggca gcgacagtac atgaccgctt tgaactgacc
    11521 aacgaggcat ctgatttgat gtatcacctg ctggtgttgt tgcaggatca agggctggat
    11581 ctcggagaag tgattgataa tctgaagaat cggcattaac gcgttggccc ccgcagcaac
    11641 ggcgggggta gcatctctca ggatttgctt tccagcaatg tagtgtagag attgacatag
    11701 gcgtcaatca tatggtcggc agtaaacatc tcttcaaaac gacgacgcga gttttcgcca
    11761 tagcggttgg aggtttcctc gttattccag agctcattca tcgcctccac cagcgcctgg
    11821 ctatcattcg gcggaatcac gcaaccactc actttgtcct ggttaatgaa agaggtacct
    11881 gtaccaatct cacaagagat cagtggcctt gcgaagcgtg cgccttccaa taacgtaata
    11941 ccaaacgcct cagagcgcag atgcgagggg aataccacgc cccggcagag ctggaagaga
    12001 atgtacttat cttcgtcgtt gagcatgccg gtaaacatca cattgctcag cccgcgctgc
    12061 tgcgcttcac gccgcacttc cgattccagc ggcccgcccc ctacaaccac cactggcagt
    12121 cggctacgct cagcggcatc catcagaata tgcagccctt tgtagtagcg gaaagtgccg
    12181 acgaagagaa agaacttatc gccgacagtt tcccgccagt gcgcgaccct ctgcggatcg
    12241 tgctgcacgt cctgctgttc cagaccaaac gggatcacca ccgttttatc cagatatttt
    12301 ttcagggtct ggctggaagc cacataattg ggcgacgagg caacgatgca atctacgccg
    12361 ctgagaaatc gctcctgcag cggctggtac agcttcatta accgtttttg tttcactata
    12421 tcagagtgat aggtcaccac agtcctggcg tcaggccgcg ccgaaagatg cagcatatcc
    12481 ataaagggaa acggaaaatg gtagttgatg atatcgtagt catctttaat cagcttaaaa
    12541 cgctttaacg ctttcagcga aaacggcgtg gaggcaattt caaaaagctg tttattataa
    12601 atgacccgat gatcttcgta agcgacagga cctgtatctt tgtccgggct aaaagtgaaa
    12661 acatcggctg cgattccccg gcgggcgcag ccctgactta gctgataaat tacctgctca
    12721 ataccgccgt aggtatcggg atagtaagtc ttatagacgt gtagaactct caactgacac
    12781 ccttagagta atttataggc gttaatggtc tgtgtcgcac agttctccca cgaaaactgt
    12841 ttcgcctgag caagaccgcg cgcggtggcg atttcccgcc agctatcatc ttgcaggctc
    12901 tgcaaaattt gcgcgctaat cgcgtctata tcattaggat cggcaacgag gccggcatcg
    12961 ccaacaacct caggcaaaga ggtgacattg gagcatacta ccggcacacc gcaagacatc
    13021 gcttcaagaa taggtaaacc gaatccctcg tagaaggagg gataaacaaa gactctggcc
    13081 gctgcgtaca gatacggcag gtcttcatcc ggaacatatc cgaggtaacg gatccagcct
    13141 tcccgagtac cgagctcgac taactgccac agcacatcgt cttcccagcc gcgatagccg
    13201 ctaaggatta gcggatagcg catgcggatc tccatcggta gcagctgata ggcatgcagc
    13261 aggcctcgga tatttttacg tggctccata gtgccgatat atagcgcgta ggcctgccac
    13321 gccagctgat atttctgcag taccggcaga cactctgccg ggctgcgtgg gatatagtca
    13381 ctgctgcagg ctagcttggt ggttacgatc cgctcctccg gatagttgaa caagcggata
    13441 atttcactgc gcgaaaaatc agaaacggtc aggatcagct ttgccgaatc cagactctca
    13501 tgcagggact tctccatata gcgaacccga tcttttggat gatattccgg gcaggtaaaa
    13561 atggatatgt catgaaacgt ggtcacggcg cgttccagtt tatgcggcag ataaaaattg
    13621 gggccatggt agatataatc cttatagtcg cgcaatgccc acgcctggcg ccgcggatgc
    13681 agcaagcgat aagcctcaat caacagcgtc tgtcggcgta gaaacgccga cagacgacca
    13741 tgattgctgg ctttggtatc gcttttattc tccaccaaag ggatctgttc tataaacgac
    13801 gcaccgtgaa atagctttaa ttcttcaatt tcgcgggcga ccgccagccg cttaaccagc
    13861 tccagggaat accgaccgat gcccgttaat gggtatttaa ttggctcagt agcaaaaata
    13921 attttcataa t
//
LOCUS       DBANUU010000117        13897 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000117 DBANUU010000000
VERSION     DBANUU010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.118"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            93..659
                     /locus_tag="ACIE68_003539"
     CDS             93..659
                     /locus_tag="ACIE68_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5909799.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            710..3169
                     /locus_tag="ACIE68_003540"
     CDS             710..3169
                     /locus_tag="ACIE68_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFX5909800.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTTGINAGAWRLRSDYQLNQTDSDDNHEQSGGIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDERMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3184..3915
                     /locus_tag="ACIE68_003541"
     CDS             3184..3915
                     /locus_tag="ACIE68_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFX5909801.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3912..4973
                     /locus_tag="ACIE68_003542"
     CDS             3912..4973
                     /locus_tag="ACIE68_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5909802.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5125..6171
                     /locus_tag="ACIE68_003543"
     CDS             5125..6171
                     /locus_tag="ACIE68_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFX5909803.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6168..6959)
                     /gene="nei"
                     /locus_tag="ACIE68_003544"
     CDS             complement(6168..6959)
                     /gene="nei"
                     /locus_tag="ACIE68_003544"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFX5909804.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYCVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6995..7729)
                     /gene="pxpA"
                     /locus_tag="ACIE68_003545"
     CDS             complement(6995..7729)
                     /gene="pxpA"
                     /locus_tag="ACIE68_003545"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFX5909805.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMHACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEQQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7719..8651)
                     /gene="pxpC"
                     /locus_tag="ACIE68_003546"
     CDS             complement(7719..8651)
                     /gene="pxpC"
                     /locus_tag="ACIE68_003546"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFX5909806.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8645..9301)
                     /gene="pxpB"
                     /locus_tag="ACIE68_003547"
     CDS             complement(8645..9301)
                     /gene="pxpB"
                     /locus_tag="ACIE68_003547"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFX5909807.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9324..10067)
                     /gene="ybgI"
                     /locus_tag="ACIE68_003548"
     CDS             complement(9324..10067)
                     /gene="ybgI"
                     /locus_tag="ACIE68_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFX5909808.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10338..11819
                     /gene="dtpD"
                     /locus_tag="ACIE68_003549"
     CDS             10338..11819
                     /gene="dtpD"
                     /locus_tag="ACIE68_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFX5909809.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVQMIWLYQALKFRNRALALES"
     gene            complement(11861..13279)
                     /gene="phrB"
                     /locus_tag="ACIE68_003550"
     CDS             complement(11861..13279)
                     /gene="phrB"
                     /locus_tag="ACIE68_003550"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFX5909810.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLRERMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMISASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13276..13785)
                     /locus_tag="ACIE68_003551"
     CDS             complement(13276..13785)
                     /locus_tag="ACIE68_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFX5909811.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT         3352 a         3727 c         3410 g         3408 t
ORIGIN      
        1 aatattttta agaaaaacta aaaacattcg acaatcaaat aagatattct tgcaccgata
       61 tacttttttc ttttctgtta aaggactccg ttatgtttaa aggacaaaaa acattggccg
      121 cactggccgt atctctgctg ttcacagcac ctgtttatgc ggctgatgaa ggatccggtg
      181 aaattcactt taaaggtgaa gttattgaag caccgtgtga aatacatcag gatgatattg
      241 ataaagaggt tgaactcggt caggtgacca ccagccacat taatcagtca catcacagcg
      301 atgccgttgc tgtcgacctg cgcttagtca actgtgatct ggaaaactcc agcaacggtt
      361 ccggtggcaa gatttctaaa gttgcagtca cctttgatag ctcagcgaaa accaccggcg
      421 cagatccgat tctcaacaac accagcacgg gtgaagccac cggcgtcggc gtacgtttaa
      481 tgaataaaga tcaaagcaat atcgttttag gcaccgctac tccagatatc gacctggctc
      541 cgacctccag cgaacaaacg ctgaatttct ttgcctggat ggaacaaatt gatcaggcta
      601 cacctgtaac gccaggcgca gttacggcga atgcaaccta cgtgctggat tataaataag
      661 tattactttg gcggcgctat cgccgccttt cataaaatat ttatcaggta tggacaccgt
      721 gaatatttat cgactctctt ttgtatcctg cctggtcgtg gcgatgcctt gcgcattggc
      781 ggtcgaattc aaccttaatg ttctcgataa atcaatgcgc gaccgcattg atatttcatt
      841 attaaaggaa aaaggagtca ttgctcccgg tgagtatttt gttagcgttg cggtaaataa
      901 taaccaaatc agtaacgggc aaaagattaa ctggcacaaa aatgacgata aaaccattcc
      961 gtgcatcaat gatttactgg tcgataaatt tggcttaaaa cctgaagtcc gtcagtcgtt
     1021 accattgata aatcagtgcg tcgattttag ctcccgacct gaaatgctct tcaatttcga
     1081 tcaagccaat cagcaactaa atatcaccat tccgcaagcc tggctggcgt ggcactcaga
     1141 aaactggacc ccaccctcca catggaaaga aggtgtcgcc ggtgtcctga tggattacaa
     1201 cttgtttgcc agcagctacc gcccacagga tggcagcagc agcactaacc tgaacgccta
     1261 cggtaccacc ggaattaacg ccggggcatg gcgcttacgt agtgattatc agttgaatca
     1321 gactgatagc gatgataacc atgaacagtc aggcggaata tcgcgcacct atctttttcg
     1381 tccattaccg caattaggct ctaaattaac cctcggcgaa acggatttta gttccaatat
     1441 tttcgacggt ttttcttata ccggcgcggc actggcaagt gacgagcgaa tgttgccatg
     1501 ggaactacgc ggctacgccc cacaaattag cggtattgca cagaccaatg ccacggtgac
     1561 gatcagtcaa tcaggccgcg tcatttacca gaaaaaagtc ccaccaggcc catttatcat
     1621 tgacgacctt aatcagtctg ttcagggcac actggatgtc aaagtgacgg aagaagatgg
     1681 tcgggtgaac aatttccagg tttcggcagc atcgacgccc ttcctgactc gtcagggaca
     1741 ggttcgctat aaactggccg cgggtcagcc acggccctcc atgtcacatc aaactgaaaa
     1801 tgaaaccttt tttagcaatg aagtttcctg ggggatgctg tcaaacacct cgctgtacgg
     1861 cggcctgctg ctttctggtg atgactacca ttctgccgca atgggtattg ggcaaaatat
     1921 gctgtggctt ggtgcgctgt cgtttgatgt cacgtgggcc agtagccatt ttgatactca
     1981 gcaggacgag cggggcttaa gctaccgttt taattacagc aaacaagtgg atgccaccaa
     2041 cagcacgatt tccctcgccg cttatcgttt ctccgatcgt cattttcaca gctacgccaa
     2101 ctatcttgat cacaaataca acgacagcga tgcgcaggac gaaaaacaga cgatcagctt
     2161 atctgtgggc caaccgatta ccccactaaa cctcaatctt tacgccaacc tgctacatca
     2221 aacctggtgg aatgcagacg cctccacgac cgccaacatc acggcaggtt ttaatgttga
     2281 tattggtgac tggagagata tctctatttc gacgtcattc aatacaaccc attacgaaga
     2341 taaagatcgc gacaaccaga tttacctgtc gatttcgctc cccttcggta acggtggtcg
     2401 ggttggctat gacatgcaaa acagtagcca cagcaccaca caccgcatgt cgtggaacga
     2461 tacgctggat gaacgtaata gctggggcat gtctgccgga ctgcaatccg accgtccgga
     2521 caatggagcc caggtgagcg gtaactatca gcatctgagt tcagcgggtg agtgggatat
     2581 ttctggtacc tatgccgcca atgattacag ttccgtcagc agcagctgga gcggttcttt
     2641 caccgcaacc caatatggtg cagcgtttca tcgccgcagc tccaccaatg aaccacgcct
     2701 gatggtcagc accgatggcg tggcagatat tccggtccag ggcaatctcg actacaccaa
     2761 ccattttggc attgcggtgg tgccgttgat ttccagttac cagccttcca ccgtggcggt
     2821 gaacatgaat gacttacccg acggcgtaac agttgcagaa aacgttatca aagaaacgtg
     2881 gattgaaggc gcgataggtt acaaatcact ggcttcccgt tccggtaaag acgttaacgt
     2941 catcattcgc aacgccagcg gtcagtttcc tcccctcgga gcggatatcc gccaggatga
     3001 cagcggcatt agcgtgggga tggttggcga ggaaggacat gcctggttaa gcggagtcgc
     3061 tgaaaatcaa aagtttaccg tggtctgggg tgatagccag cattgctcgc tccatcttcc
     3121 tgaacatatg gaagacaccg caaatcgcct gattttacct tgtcattaat taaggaaatg
     3181 aaaatgacat ttatgaaagg actacccctt ttgttgttgg ttgccagtct gtgcagccac
     3241 gctgcactac aacccgatcg cactcgtatt gtgtttaacg ctaatgataa agctaccagt
     3301 ctgcgggtgg ataatcgtag cgataaactc ccctacctcg cttattcctg gctcgaaaat
     3361 gaaaaagggg agaaaagcga tgatcttctg gtcgccctgc cgccgataca acgtctggaa
     3421 ccgaaagcaa caacgcaggt acggatagta aagcaagcct cgaccacaaa attaccgggc
     3481 gatcgcgaaa cgctattttt ctacaatatg cgtgaaattc ctcccgcccc ggaaaaaaac
     3541 agcgaccatg ccgttctgca agtcgccatt cagagtcgta tcaaggtgtt ctggcgaccg
     3601 gctgcattac gcaagaaagc aggagaaaag gtcgaactgc agttacaggt cagccagcag
     3661 ggcaaccaac tgacactgaa aaatcctacc gcgtattatc tgaccattgc ctacctggga
     3721 cgaaatgaaa aaggcgttct ccccggcttc aaaaccgtga tggttgcgcc ttttagtacg
     3781 gtcaacacaa acaccggaaa ttatagcggc agccagtttt atctcggtta tatggatgat
     3841 tacggcgcgt tacgcatgac gacgctcaac tgcagcggac aatgccattt acaagccgtg
     3901 gaggcgaaga aatgagtgct ggcaagggag tgttgctcgt catctgtctg ttatttctgc
     3961 cgttgaagtc tgcgcttgcc ctgaactgct actttggttc atccggtggc tcggtagaaa
     4021 aatcagaagc gattcaacct tttgctgtac caggcaatgc caaactcggc gataagattt
     4081 gggaatctga cgatattaaa attcccgtct attgtgacaa caataccaac ggcaattttg
     4141 aaagtgagca tgtttatgcc tgggtaaacc cctacccggg ggtgcaggat cgctattatc
     4201 aactcggcgt aacttacaat ggcgttgatt atgacgccaa ccagggaaaa agccgcatcg
     4261 acactaacca gtgtattgac agtaagaaca ttgacattta tacccctgag cagattatcg
     4321 cgatgggatg gcagaacaaa atttgctcgg gtgatcccgc taatattcac atgtcgcgta
     4381 cctttctcgc tcgcatgcgg ttatacgtca aaatacgaga aatgccgccg catgattatc
     4441 aaagtacgct tagcgactat atcgtcgtgc aatttgacgg tgccggtagc gttaacgaag
     4501 atcctactgc ccaaaacctg aaatatcata ttaccggtct ggaaaacatt cgcgtgctgg
     4561 attgcagcgt caatttttcc atttcaccgg aaacacaggt gattgatttt ggtaaattta
     4621 atttgctgga tattcgtcgc cacacaatgt cgaaaacatt cagtattaag acgacaaaat
     4681 cacaaaatga ccaatgcacc gacggattta aagtcagttc ctccttttat accgaagaaa
     4741 cgttggttga agaagataaa gcattgttga ttggtaatgg tctgaagcta cgtttgctgg
     4801 atgaaaacgc ctcgccttat accttcaata aatatgctga atacgccgat ttcaccagcg
     4861 acatgttggt ctacgaaaaa acctatacag ctgaactttc gtccattgca ggtaccccca
     4921 tcgaggctgg gcccttcgat acagtggtgc tttttaaaat taactataac tgatttgcca
     4981 tgatgccctt tgcagccgga aagggcatct ctttcagaaa attgctgatt ttcccctttt
     5041 ccttgattgc tgttattttt tcctggcccg gctttcttta tgctgtatcg atacaccaaa
     5101 gagaaaataa tgagggagcg aaggatgcca gttttgcagt ggggaatgtt atgtgtgcta
     5161 tcacttctcc tttctattgg tttcctcgcg gtacatctcc cggcggcgct attacttggg
     5221 ccgatgatcg ccgggatcat cttcagtatg cgcggaatta ctctgcaact tccccgctcc
     5281 gcttttctcg ccgcgcaggc cattcttggc tgcatgattg cgcaaaacct cactggttcg
     5341 attctcacca cgctggccgc caactggccg atcgtgcttg ctattttact ggtgacgctg
     5401 ctttccagcg ccatcgtggg ttggttattg gtgcgctata gctcactgcc tggaaatacc
     5461 ggtgcctggg gttcttctcc aggcggcgca gcggcaatgg tcgctatggc gcaagattac
     5521 ggcgcagata ttcgcctggt ggcgtttatg caatatctgc gagtgctgtt cgtcgcgggg
     5581 gccgctgttc tggtcacccg catgatgctg ggtgataacg ctgaagcggt taaccagcaa
     5641 atcgtctggt tcccgccagt gagcattaat ctcctgctta cgattttgct cgcagtcgtt
     5701 gccggtacgg cgggatgcat gctgcgtctt ccttccggca cgatgctcat cccgatgctg
     5761 gcgggcgcag tgctccagtc tggtcagctc atcaccatcg aactaccgga atggctgctg
     5821 gcgatggcgt atatggcaat tggctggcgg attggtcttg gtttcgataa acaaatctta
     5881 ctgcgggcac tacgcccact accgcaaatc ctgctgtcga tttttgctct gctggctatt
     5941 tgtgcaggta tggcgtgggg actgacccgg tttatgcata ttgattttat gactgcctac
     6001 ctcgccacca gtcccggcgg gcttgatacc gtggcggtca tcgccgcagg gagcaatgcc
     6061 gatatggcgc ttattatggc gatgcaaacc ctgcgcctgt tcagtatttt gctgacgggg
     6121 ccagccattg cgcggtttat ttcaacctat gcgccgaagc ggtcggccta gtgctggcag
     6181 cctggacacc agtaaaacgg gcgcgaggac aacgtggttt tctcaatgat gccgccacag
     6241 cgttcgcaca gttcgccatc acgatgaaaa accttaaagc gaaacagcgc cccatgatgc
     6301 ttattctcat ccacctgccc ccgcgtagcg taagaaaacc gaggaatctc cagtaacgcg
     6361 tgtgcgagtg catccagttg cgccgcattg agatctttcg ctttatgatt tccagtcaac
     6421 ccaacctgcc agaggatctc cacccgcaaa taattgccaa gcccagccag aaacgcctga
     6481 tcgagcagta atcctgcaaa ctgacggtta cgaaagcgcg gtgacaataa ccgttccttt
     6541 accacctccg gcgtcagatt cggatccagc acatcgggac caacgcgttg taaaaacgga
     6601 tgcgtggtca gttgttccgg ggtcaacatc tcaatatccg aggcgctata aagcagaatg
     6661 gttttgtcag ccgtttgcag ttttacccgc aatactcgcg tggtctgcgg ctcttcgccg
     6721 gtatcaacca cgcgccagac gcagtaaagc tgattatggc tgtagagcgt taagtcgttg
     6781 gaaaaatgag ttaacaacgc cttaccacgc gtttccacat gggtaacgtg ttgaccgata
     6841 agttgtgatt gataagtttt taactgcggg aaggcaaacc agacatcagt tagtggtttg
     6901 cctttgatcg ccgcctccag gttatccgct gcacggcgga tctccgggcc ttcaggcatg
     6961 gtgatatcct tttttcattc tttattaagc ggggttatgc tgcgacaaca atcccctttt
     7021 tggcaaatgt agctcgtaat cggcgggcga acgccagagc gtgctcgccg tcgccatgca
     7081 ggcagaccgt ttgcgccgcg accgttgccc attcgccggt gatacttttg actctgccgt
     7141 gttgcaccat ctccagcgtt tgcgccagcg cctgttgttc gttttcaatc aacgcgcctc
     7201 gctgacttcg cggcaccagc gagccgtcag cctgataacc gcgatcggca aacacttcct
     7261 cgcgcgttgt caggccatat tgcttgcctg cacgaatcag ctcgcttcct gccagcccaa
     7321 cgagaatcaa tgccggatcg caagcgtata ccgctctggc gatggcgtct gccagttgtg
     7381 cttctttcgc cgcctggttg tacaacatgc cgtgcggttt gacatgacgc attacgccgc
     7441 cttgcgcacg ggcaatcgtt gccagcgcgc caatttgata cagcgtctgg gcgtaaacgg
     7501 tttctggcgg cagctgcatg gcgctgcgac caaaattttc cctgtcggga aaactcgggt
     7561 gagcgccaat tgcaacacca ttttttattg cttcacgcac gcaagcatgc atggtttgcg
     7621 catcgcctgc atgaaatcca caggcaatat tggcagagga aaccagcgtt aatagctctg
     7681 cgtcgctggc gcagccttcg cccagatcgg cgttcaggtc aattttcatt gtgcagccgc
     7741 cacgctaatt gttcaaaata acgttgctga tcttgccgcg ctttcagtgc ctcttccagt
     7801 gaacactgga caaaatgaat cggctgaccg agcggaattt gcgccagatg gtacatatca
     7861 gcctcaatga tgcaggcaat acgcgggtaa ccaccggtgg tctgtgcgtc gttcatcaac
     7921 acaatcggct gcccgttatg tggcacctgc accacgcccg gtaacaaacc gtgagataac
     7981 agttcgcgat cggtggtgcg ttttaaaatt tgcccctgta agcgatagcc catgcggtta
     8041 ctttgcgggc taagctgcca gggcgaacgc cagaatgcat cctgcgaggc gcgatcgaac
     8101 tcatgatatt ccggccccgg caaggcgcga atgcggttgc cccacagcag ctgtttaacg
     8161 ccctgcgctt ccataaaatc acgcttcgat ttgccaatcg ggagtcggtc accatccttc
     8221 agtaaacggc cttccagccc gccaatcccc acgttgagat cggtgctgca tgaccccatt
     8281 accggcggaa catcaatacc acccgcgacc gccagataac tgcgcatccc gtgctgcggg
     8341 cgtttaagcg ttaaacgctg gcctgctttc atcggcaatc gccagccggt ccagacggca
     8401 ttatcatcca gccgcgcttc gcaaccggca cccgtcagag caaaccaccc atcagtttcg
     8461 aactcaacag ttaactgacc gagcgtgatc tccagcgcgg gggcattggc gtcattaccc
     8521 accagtaggt tagcaatgcg taacgcgggc atatccagtg cgccgcagtg gctgataccc
     8581 gactggcgaa aaccgtgacg accgccatcc tgcacagtgg tatacatgcc cgcacgaata
     8641 atcttcagca tactccctcc ttctgcggta caaagcgcac gctgtctccc ggacgtaata
     8701 agatgggttc gtcacgcgcc ggatcaaaca ggctgagtga ggtatgacca atcaactgcc
     8761 agccacccgg cgttgccagc ggataaacac cagtctgcgg cccgccgatc ccgacagaac
     8821 ctgccggaac gagtaagcgc ggttcagcgc gccgtggcgt gtgtagttgt tccggcaaac
     8881 tcccgagata cgggaagccc ggttgaaaac ctaaaaacca gaccacgtat tccacggagg
     8941 agtgcaattc aacaacctgt ttttcgctca acccgcaatg cgccgcgacc accgccaaat
     9001 ccggtccgcc tgcaccaccg taaaccaccg gaatttcaat aaagcgagac tcaggttcca
     9061 gggcctcgct ctcctcccac cagcgttgca aacgctctat ggcatccagc gccagcgact
     9121 caggattacg caaaatcacc gtaatattgt tcatgccggg aatggcttca accacattcg
     9181 gcatatccac cagacgctgc gccagtcgcc agatccgttt ctggctagcc agcgtcaccg
     9241 gcggttccag ttccagcact accgccgttt cacctatcag ataacaacgc gctcgttgca
     9301 ctttcgatcc ctctgattat tcgttatgca ggattaggaa tatcaataaa ggtcacatca
     9361 agatcggtat tttcattcag ccactcgctc aatgcgcgaa taccaccacg ttcagtggca
     9421 tggtgacctg cagcataaaa atgcaatccc tgctcgcggg ctgaatgaat ggtctgttca
     9481 gaaacttcgc cagtaataaa agcatccacg ccaaaacgcg cggcgctatc gataaaactt
     9541 tgcccgccgc ccgtgcacca ggcgacgcgc tggaccacct caggtccggt atcgccacac
     9601 cataacggct tacgtcccag acgcgcttca atccaggagg ctagttccag tcccggcaca
     9661 ggcatggtca gctctcccca cggcaccaac ggctcaattt cgcccatgac cgtgatcccc
     9721 agtaacgccg ccagctgtgc gttattgccc agctcaggat gtgcgtcaag cggcaaatgc
     9781 cagccataca ggttgatatc attcgccagc aacgttttta aacggttacg cttcattccg
     9841 cgaatgaccg gagactctcc tttccagaag tagccatgat gcacaatgac cgcatccgcg
     9901 cccagacgca ctgcctcatc gagcaaagcc tggctggcgg tgacaccggt aacaattttt
     9961 tgcaccgtct ctttgccttc cacctgcaaa ccattcggcg cgtaatcact aatcgccgcg
    10021 ctgttcagtt tttcgttaat cagttgttcc agttcggtgt ttttcatctt cgcgccttcc
    10081 gtattctgat agctacaccg ttaacatagc gttacctcgc ctgttcgtcg atagccaatc
    10141 ttttgacata ttaagaaaca aacccgacgc caaaagacgc aaattgttta tatcatcacg
    10201 cttcagctaa tgtatattta tgcaatcaga cagcatattg cataaataat cagatctgta
    10261 tcaaagtttt aactttaagg aaacacaagc acgccgcgca actattacgt gacgttgccg
    10321 tcaattgtgg aattattatg aataaacacg catcacaacc gcgcgctatt tactatgtcg
    10381 ttgcgctgca aatctgggaa tattttagct tttacggcat gcgtgccctg ctgattctct
    10441 atctcaccaa tcaactaaaa tacaacgata ctcacgccta cgagttattt agcgcctact
    10501 gttcgctggt gtatgtcacg ccaatcctcg gtggcttttt ggcggataaa gttctcggca
    10561 atcgcatggc ggtgatgctg ggggcgttgt tgatggcgat tggtcatgtg gtgctgggtg
    10621 ccagtgagat ccatccgtca ttcctctatc tgtccctggc gattatcgtc tgcggctatg
    10681 gcctgtttaa atctaacgta agctgtctgc tcggcgagct gtatgagcca accgatccgc
    10741 gtcgtgatgg cggtttctcg ctgatgtatg cggcgggtaa cgtggggtct attatcgcac
    10801 ctatcgcctg tggttacgcc caggaagagt acagctgggc gatgggcttt ggcctggcgg
    10861 cggttggcat gatcgcgggt ctggtcattt tcttatgtgg caatcgtcat ttcactcata
    10921 cccgcggcgt taacaaaaaa gtactgcgtg cgacaaactt tctcctgccg aactggggat
    10981 ggctgctggt tctgctggtg gcaacgcctg cactgattac cgtactgttc tggaaagagt
    11041 ggtcggtata cgccttaatt gtcgcgacta ttattggcct gggtgtactg gcaaaaattt
    11101 atcgcaaagc agaaaaccag aaacagcgga aggagctggg gctgattgtg acgctcacct
    11161 tcttcagtat gttgttctgg gccttcgcac aacagggcgg tagctcgatt agcctttata
    11221 tcgaccgctt cgttaaccgc gatatgtttg gttataccgt tccgaccgcg atgttccagt
    11281 cgattaatgc cttcgcagtt atgctgtgcg gtgtgttcct ggcgtgggtg gtaaaagaga
    11341 gtgtcgcggg taatcgtacc gtgcgcatct gggggaaatt tgctcttggc cttggcctga
    11401 tgagcgccgg attctgcatt ctgaccttaa gcgcccgctg gtccgcaatg tatggtcact
    11461 cttctctgcc actgatggta ttaggcctgg cggtgatggg ctttgcggaa ctgtttatcg
    11521 acccggttgc catgtcgcaa attacgcgca ttgaaatccc cggtgtgacc ggcgtattaa
    11581 ccggcatcta catgctgctt tctggcgcga tcgcgaacta tctggcgggc gtgattgccg
    11641 atcagacatc gcaggcttcg tttgatgctt ccggggcgat caactactcc atcaatgcat
    11701 atattgaagt atttgatcaa attacctggg gcgcactggc gtgtgtagga ctggtacaga
    11761 tgatttggct gtatcaggcg ctgaaattca gaaaccgcgc gctggcgctg gaatcttaat
    11821 aggccagcac catacctgat aaaacgctcg ctgcgtttta tcaggctcct ttcctcgctt
    11881 cttcgtacgc tgccagcgtt cgcagccttg cctctttgtg atcaactatc ggttgcggat
    11941 aatccagcat cacacctgct ttctgcgccc acttccacgg ctcatgcacc gctttccctg
    12001 gtacatcgcg cagctccggt aaccatcggc ggataaactc accttcgcga tcaaatttct
    12061 cgccctgggt tgtcgggttg aaaatacgaa aatacggcgc tgcatcagta cccgtagacg
    12121 ccgcccactg ccagccaccg ttattggctg ccaaatcacc atcaatcagc tgcgacatga
    12181 aatatcgctc gccttcgcgc cagtcgatca acaaatcttt caccagaaaa ctggctgaaa
    12241 tcatccgtag ccggttatgc atccagccag tgctgttaag ctgacgcatg gcggcatcga
    12301 caatcgggta tcccgttttg ccttcctgcc aggcctgtaa atgtgcggga ttgctctgcc
    12361 actgcacacg atccgtccag gcaataaacg gacaatgttt acacaacgag gggtaatacg
    12421 tcatcagatg acggtagaac tcgcgccaga tcagctcact aagccagaca ctaccggccc
    12481 caccgtccag cgcctgcggc tgttccgcca acaagcgatg caaacactgg cgaggcgata
    12541 acccgcccgt tgccaggctg gcggacaaac gactggtgcc ttccactgcc ggaaaatctc
    12601 gttgttgctc atattctccg gcaccgttct ggcaaaactg gcgtaattgc gcaatcgccg
    12661 ctttttcttc caccgggaaa tgcgcagtat cgaaagactg acgaggataa ttcagcgtaa
    12721 tggatggcga gggctctatc gatccgctac tacgaacttt tggcgcagcg acgcactccg
    12781 gcatcctttc ccgcagccgt ttcagccagg cattcttaaa aggcgtaaag actttgtaca
    12841 tctcgtgatt accggtcatc accgcgccag gcggcaggat cacgctgtca tcaaatcctt
    12901 cacacaccac gttacgcagc gctctttcaa cttccacatc ccgcgcccgc tcattcactt
    12961 catactgata gttataaaac aggtgggtaa cgctgttttc cgcgcacacc tgtttaacta
    13021 tttcgacact ggcgacaaag tcatccactt cacggaacaa taaaggaata cctttttccg
    13081 caagcgctat ttgtagccca ttcagttgag cattgataag ttcagcctga cgcggcgaca
    13141 tgttatgcgt cgcccactgg cgtggtgtag cgatatacaa cgccagcacg cgtgcagacg
    13201 aattgcggca ggcggcagcc agtgcgagat tatcgtgcag acgtaaatcc tggcgaaacc
    13261 agaccagatg ggtagtcata aaactcctga atggcgcaag ttaatcacac gcggccaaag
    13321 ttcgaaataa cgctgccctg aaagccaggc gtcaggataa agcgccatat agtgtttgat
    13381 acgcatcagc ggcgcaagaa gtggttgctc gccacggcga taactgtcga taagcgcagc
    13441 cagctgctgg cgttctgtgg aatcaatatg cgggcgaaaa taaccctgaa cgtgcatcaa
    13501 aacattggtg tgatcgcgcg ggttggcggg gtgagaaagc aaaacaacta cgcgttggcg
    13561 gtactggttg taatagtcat ccagattttg ccactggtga atatcagcca cgaacgggcc
    13621 aagtttgcgg tactccggct gagaatgcgc cagaaagacg agtttatagc ggctgtgata
    13681 atcgagaagc gccccgcgcg ttaagccatg ttctttcagt cgatgtaact catgaagggc
    13741 aaagatacgg attagggtgc tgtcatcaaa tcgttgtaga ttcattactg ctcctgaacg
    13801 gtataaagga gtaagtgtag acgatggtaa atgaatttta tggggcagag tttttgcggg
    13861 gaagtatttt gttggtaaaa atattatcaa cttcatc
//
LOCUS       DBANUU010000118        13757 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000118 DBANUU010000000
VERSION     DBANUU010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.119"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..284
                     /gene="ghoS"
                     /locus_tag="ACIE68_003552"
     CDS             <1..284
                     /gene="ghoS"
                     /locus_tag="ACIE68_003552"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFX5909812.1"
                     /translation="KFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQ
                     LNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            312..485
                     /gene="ghoT"
                     /locus_tag="ACIE68_003553"
     CDS             312..485
                     /gene="ghoT"
                     /locus_tag="ACIE68_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFX5909813.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(604..2121)
                     /gene="lysS"
                     /locus_tag="ACIE68_003554"
     CDS             complement(604..2121)
                     /gene="lysS"
                     /locus_tag="ACIE68_003554"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFX5909814.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(2358..3815)
                     /gene="dtpC"
                     /locus_tag="ACIE68_003555"
     CDS             complement(2358..3815)
                     /gene="dtpC"
                     /locus_tag="ACIE68_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFX5909815.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMVKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(3874..6021)
                     /gene="cadA"
                     /locus_tag="ACIE68_003556"
     CDS             complement(3874..6021)
                     /gene="cadA"
                     /locus_tag="ACIE68_003556"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFX5909816.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(6101..7435)
                     /gene="cadB"
                     /locus_tag="ACIE68_003557"
     CDS             complement(6101..7435)
                     /gene="cadB"
                     /locus_tag="ACIE68_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFX5909817.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(7801..9339)
                     /gene="cadC"
                     /locus_tag="ACIE68_003558"
     CDS             complement(7801..9339)
                     /gene="cadC"
                     /locus_tag="ACIE68_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFX5909818.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(9599..9713)
                     /locus_tag="ACIE68_003559"
                     /pseudo
     CDS             complement(9599..9713)
                     /locus_tag="ACIE68_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(10088..10657)
                     /locus_tag="ACIE68_003560"
     CDS             complement(10088..10657)
                     /locus_tag="ACIE68_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFX5909819.1"
                     /translation="MSEITVSRPEVVNENTDVICSTSVRSRSLEYDNFPEISEANILS
                     TFEQLHQNKDEVFERGVINVFKGLSWDYKTNSPCKFGSKIIVNNLVRWDQWGFHLISG
                     MQADRLADLERMLHLLSGKPIPDNRGNITINLDDHIQSVQGKGRYEDEMFIIKYFKKG
                     SAHITFKRLELIDRINDIIARHFPSVLSA"
     gene            complement(10754..10951)
                     /locus_tag="ACIE68_003561"
     CDS             complement(10754..10951)
                     /locus_tag="ACIE68_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFX5909820.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            complement(10963..11451)
                     /locus_tag="ACIE68_003562"
     CDS             complement(10963..11451)
                     /locus_tag="ACIE68_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFX5909821.1"
                     /translation="MKLSLMLEANRINVQALNMGRIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(11448..11822)
                     /locus_tag="ACIE68_003563"
     CDS             complement(11448..11822)
                     /locus_tag="ACIE68_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFX5909822.1"
                     /translation="MNTLPDTHVREASHCQSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            complement(11912..12280)
                     /locus_tag="ACIE68_003564"
     CDS             complement(11912..12280)
                     /locus_tag="ACIE68_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFX5909823.1"
                     /translation="MSDTLPGTTHPDDNNDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKRLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            complement(12360..12581)
                     /locus_tag="ACIE68_003565"
     CDS             complement(12360..12581)
                     /locus_tag="ACIE68_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFX5909824.1"
                     /translation="MKIIPRGEAMRIHRQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(12668..13144)
                     /gene="radC"
                     /locus_tag="ACIE68_003566"
     CDS             complement(12668..13144)
                     /gene="radC"
                     /locus_tag="ACIE68_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFX5909825.1"
                     /translation="MQQLSFLPGEMTTRERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(13160..13645)
                     /locus_tag="ACIE68_003567"
     CDS             complement(13160..13645)
                     /locus_tag="ACIE68_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFX5909826.1"
                     /translation="MTTFSHSSTTPSVSVTTASGNNQSQLVATIVPDEQRISFWPQHF
                     GLIPQWVPLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDEDDDETWVLFNA
                     MNGNRVEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
BASE COUNT         3714 a         3201 c         3284 g         3558 t
ORIGIN      
        1 acaagttcaa tacttatgtt gtttctttta attatccatc atcttattcc tcagtgttct
       61 taaggttaag atcattgatg tatgatatga atttctcctc tatcgtggct gatgaatatg
      121 ggataccacg acaattgaat gaaaactcct tcgcaataac gacatcgtta gccgcaagtg
      181 aaatcgaaga tttaatcagg cttaaatgct tagacttacc ggatattgac tttgacctca
      241 acattatgac agttgatgac tatttccgtc agttttacaa gtagccagga aggataagaa
      301 gaacataaaa aatggcacta ttctctaaaa tattaatttt ttatgtgatt ggtgtgaaca
      361 tatcctttgt cattatctgg tttatctcac atgagaaaac acatattcgt ttacttagtg
      421 cattcctggt cggaataacc tggccaatga gtctgcctgt ggcattactt ttttctctct
      481 tttaggagct gtagttgagt ggacccgctg gttatcgtga aacgaagcgt ctctcctcaa
      541 aactggattt gcggaacgat gaacgaagca gtcgcctgac tgcttcgttc attaaagtga
      601 aatttatttc tgtgggcgca tcgccgggaa gagaataacg tcacgaatag tatgactgtt
      661 tgtaaacagc ataatcattc ggtcgatgcc aatacccaga ccagcggttg gcggcagacc
      721 atattccagc gcagtcacgt aatcttcgtc atagaacatg gcttcgtcgt cacctgcagc
      781 tttagcgtta acctgttcct ggaaacgttc agcctgatct tctgcgtcgt ttaattcgct
      841 aaaaccatta ccgatttcac gaccaccgat gaagaattca aaacggtcgg tgatttccgg
      901 gttaacatca ttacggcgcg ccagcgggga cacttctgcc ggatattccg taataaaggt
      961 tggctgaatc agatgtgctt ctgccacttc atcaaagatc tccgtgacaa tacgtcccag
     1021 cccccagctt ttctctaccg taattccgat agattcagct aatgctttag cagcatcaaa
     1081 attatccagg tcggccatat cggtttctgg acgatatttt ttgattgctt cgcgcatggt
     1141 gagtttttca aacggtttgc cgaaatcaaa cacatgctcg ccataagtga ctttagtggt
     1201 acccagaacc tcttgtgcca gggtgcggaa cagtgactct gtcagttcaa tcaaatcgtg
     1261 gtaatccgca tacgccatgt agagttccat cattgtgaac tcaggattat ggcgaacaga
     1321 aataccttca ttacggaagt tacggttgat ttcgaatacc cgttcaaaac cgcctacaac
     1381 cagacgtttc agatacagct ccggcgcgat acgcagatac atatctaaat ccagagcatt
     1441 atgatgggta ataaacgggc gagcagatgc cccacctgga attacctgca tcatcggggt
     1501 ttctacttcc ataaagccgc gcgcgaccat gaactggcgg atagcggcca gaatttttga
     1561 acggacaaca aacgtttgac gggatttatc gttagcgatg aggtccagat aacgttgacg
     1621 ataacggact tcctgatcct gcagaccatg gaatttatct ggtaaaggac gtagtgcttt
     1681 tgtcagcagg cgcaactcag tacagtgaat ggaaagctca ccggtttgcg tcttaaacag
     1741 cgtaccgcgg gcaccgataa tgtcacccag atcccatttt ttaaactgat cgttataaac
     1801 accttctggc aggctatctc ttgcaacgta cagttgaata cggccaccga catcctgcaa
     1861 cgttacaaag gaggctttcc ccatgatacg acgggtcatc attcggccag caaccgagac
     1921 ttcaatgttt aaggattcca gttcctggtt atccttcgca tcaaactctt cgtgcagctg
     1981 gtcagaggta tggtcgcggc gaaaatcatt gggaaacgcc acaccttgct gacgtagtgc
     2041 cgccagtttt tcgcggcgat ttctcagttc atcgttaaaa tcaatagcct cattggctcc
     2101 ccgtgtttct tgttcagaca ttttggttcc tctaaatcca gctttcaatt tagcgtagat
     2161 aaagagacag atcggtacac tgtattatct gcctcgacta ttaataactc agtttatctt
     2221 atcgaaacta acgcttacct caagttgata tcactaataa ataaccatca aaatggttta
     2281 aatgacttat taatctagat ttgctagtgt tataaactga aagttaattt attcataacc
     2341 aattgttttt acgacagtta atcgttgctc tcctgtatca tattcgtcgg cgtgctaaac
     2401 agaaaacggg tagcaaaggc caatacgacg atgatcgcga cacaagccaa cgtccactct
     2461 cccatctgag aaaagaatcg ctggtaagct gcaattgcca taccgctaat ttgcgactct
     2521 gtcgtctgct gtgccacgac gcctgccagc cagttggcga ccgcgcctgt tgccagcata
     2581 taaataccgg ttaatacgcc agacattttc agacgcgtga tttgcgcaat cgccaccggg
     2641 tcaataaaga gttcggcaaa gcccattagc gccaacccgg atatcatcac gcccattgac
     2701 gcttgaccgt cagccgctgc atgtcgggca tcaaatgcca acaacataaa gccacaagcc
     2761 atcagcagta agccaaaggc aaacttcagc cagacgcgca atgttgagtt gccgcggctt
     2821 tctggcgacg ccagccaggc cagtacaacc ccagcgagca tcaccgcaat tgcattcacc
     2881 gactggaata gtgctgtagg tacttcaata ttgaatgcct gacgattcac aaagcgatcg
     2941 ataaacaagc tgatggtact gccgccctgt tgtgccagta cccagaacaa tgtcccgaca
     3001 aacatcaaca atacaatttg ccaaagagca cggcggtgtt cggggaattt gaccatcatg
     3061 cgggcaatga tttgtgcggc aatgaggcaa acgatcgcca gcaaatatcc ggaccagtcg
     3121 ttctccagca gcagggtaaa aaatactggg gctaaacaga gcatcaccac taaccagctc
     3181 cataccggta aggcaaattt gacgctagtg agcgcttttt tatccatact acgcgtggac
     3241 tggaaatgac gatgcccgct taagaaaatc aacaaaccga taaacatgcc gccacccgca
     3301 agggcaaagc caacatgcca tccataccac tgagcagcca ggccgcaggc gattggggcg
     3361 gcgatagaac cgatattgcc cgcagcatac agcagcgaaa aaccgccatc acgtcgatga
     3421 tcgttctcgt cgtaaagctc gccaagcaaa cagctgatgt ttgatttgaa taaaccgtag
     3481 ccacaaataa tgattgccag cgccagatac aggctaaagg ttgaatttgt atcaatgccc
     3541 agcaccacat ggccaagggt cattaacagc gcgccggcaa tcactgcagt gcggttgccg
     3601 agcaggcggt cggcaagcca gccgccgaga ataggggtaa cgtaaaccag agaagcatat
     3661 gcgctgaaca ggctgatggc atggttatca tcaaaaccaa gctgatgggt gagatagaga
     3721 atgagtaagg cacgcatgcc gtaaaaactg aagtactccc agatttggat cgccacgata
     3781 tagtatatcg cgcgcggctg tgagggtgtt ttcatgtgtt ctccttatga gcaaaaaagg
     3841 gaagtggcaa gccacttccc ttgtacgagc taattatttt ttgctttctt ctttcaatac
     3901 cttaacggta tagcggccat cagcctgacg gtatgcaccg tgaatatcgg tttcaaagcc
     3961 cggatagtga gcgccgattt cacacagcat ctgcaggaac tccagaaccg gacggctttc
     4021 ttcggtgatc atttcacccg gcattaccag aggaactccc ggcggatatg gaaggatcat
     4081 attggcgtta atacgaccta ccatttcgtc gaggtaaact tcttcggtca taccgtgcag
     4141 ctctttctgg aacgcagcat acggagtcat taccatcgtc ggcagcactt caaatgcgcg
     4201 atacatcaga tccggcagat tgtggtgaac aatcagtttg tggatattct gagccagttc
     4261 ctgaatacgc atgttttcat agaattcagg atcttcacga tacagagacg gcagcatgtt
     4321 tttcacacgc aggttcaggt cgaacgcacg tttgaagtca gtcagagcac gcagcaggct
     4381 cagtgctttg gtcttatcga taccgatgct gaacaggaac agcaggttat acggaccggt
     4441 tttctcaaca acgatgccat gttcgtcgag gtatttcgcc acgatgctgg ccggaatacc
     4501 aaagtcgctc atggtgccgt ctttttccat ccccggagtc agcagggtga ctttgatcgg
     4561 gtcaagatac atgtgctcgt tatcgatgtt tttgaagccg tgccaggtgc tgtcagaacg
     4621 cagcggccag cattcagtgg tatcgatatg atccggctgc catacatcaa agaaccagcc
     4681 atcagattcc gttctcagac gtttgatctc tttacggaat ttgatcgcac gttcaatgga
     4741 accgttgatc agacgcttac cagcattgcc tttcatcatc gccgctgcgg tttcagtgga
     4801 cgccacgata ccgtagtgcg gagaagtggt ggtgtgcatc atgtaggctt cgttaaaggt
     4861 ttcttcgttt acgtcacctt taacgtggat catggaagcc tgagagaacg ccgccagcag
     4921 tttgtgagtg gactgggttt cgtaaatcac tttcccttct acacggccac cgctcatacc
     4981 gcatttacct tcgtaaatcg gtgagaagtt ggtgtaaggc acccacgcgg agtcaaagtg
     5041 gatggatttc acatccagtg ttttcttgat aaagtcggtg ttgtacagca gaccatcata
     5101 ggtagagttg gtaattacag catgtaccgg ccaggttgcg tttggtgttt ctttcacgcg
     5161 cttagcaatg gtagcgtgct ggaattcact ctgtgggata ccaccaagaa taccgtaagc
     5221 gttacgggtc gggcggaaat agattggcgt aacatcgctc atcatcatca ggtgggtcag
     5281 cgatttgtgg cagttacggt caatcagaat ggtgctgcct gctggagcag agtacatacc
     5341 aacaattttg ttcgcagtgg aagtaccgtt ggtcaccatg tagctgcggt ctgcgttaaa
     5401 gacgcgagcg atatactgtt ctgcttcttt gtgtggacca ctgtgatcca gcagagaacc
     5461 cagttcagat actgaaatgg aaatatcaga tttcatggtg ttcggaccaa agaaatcata
     5521 gaacaggcta cctaccgggc ttttctggaa tgcagtaccg cccatgtgac caggagtaca
     5581 gaaagtatat ttaccttcac gaacatattt aaacagtgct ttagtcagcg gaggcagaat
     5641 agtgttgata tattcgtcag tggtctgctt gatcttatta gcaatatctt cagcagcacc
     5701 cagcgcatat tcaaagaagc taatctgtaa acgcaggtca ttcaggctta catcgagagt
     5761 ggaatacgta ttagcgaacg cgtacaacgg caggttctcg ttcattttgc taatttcttc
     5821 gcacagctcg agattatatt tatcccagtc aaaaataacg ccgcacagac gcgcattgtt
     5881 ttcgatcagt tttaataagt cgtcacggtc gttcgggtaa acaatctgga agttcagacg
     5941 ttcaagcgcg cgatgaagtt cacggatggg ttcttcttta aaataaaccc ccatgtgatt
     6001 caatattgca ataacgttca tagtcatatc tccaggtaaa aaaggcccct cccaacacat
     6061 gggacaaaat gaaaggagga gcctcggaaa atacttttaa ttaatgtgcg ttagacgcag
     6121 tgtggttatc cattgagtgg ctctggcgct cgtgcatttt gcgagcgtag aacatcagga
     6181 taatcaggct gacgatgaag gtacctgcca gctcgaagga gcttgcgccc atcagcgcga
     6241 tgaagcagaa cacgcaaccc agtacagagc agatcaggct gacaaagttg cggatgttaa
     6301 cgccttcaaa acgaatcagg tcaacgcaag agtagaagta cggcagcata gtcagcagta
     6361 ctgcgatacc ggtcagttca ccgaacaggt cagatgcttt accaccggca gagttcatca
     6421 gagtgatgag gatcatcagg gcagtcattt tcactgcagc cagcagcaga ccttttttcg
     6481 gaataccgtt gctgtcgact tcaccataaa ctttcgggaa gttaccgtcg ttagcggcac
     6541 gtacacctgc ctggcctacc aacatcatcc aggagcccag agaagtcagg cacgcaaagg
     6601 cggtgaatgc agaaaccagc ggcgcagccc agttaccgag gatagttgaa gcactgattg
     6661 caaacggagc accggaagcc gccattacag aagacggata cataccggaa agcacctgag
     6721 tcgcagcgat ataaacaata cctgctaaac cagtacccag catggttgcc agcggaacgg
     6781 tacgtttcgg gtttttaacc ataccagtac ttactgctgc ggattcaaca cccacgaagg
     6841 cccacaggca gagcagaata cttttaatga tcgcatgacc atcagtggta tccgcagtat
     6901 tccagttagc tgcataagtt gccgcatcaa accaatgcca gccaacaata gcagtcatca
     6961 ccacaggaat aagaaccagc accagaccaa tagtggttaa acggcttacc caggtaccgc
     7021 cgagcatatt tacaaaggta aatacccaga cgatagcaat acaggcgata cccgccggaa
     7081 caggatcatt taatactggg aagaaggtgg aaagataaga tacagcggta ataccaatcg
     7141 ccaggttacc aatccagtta gcatggtaat aaagaacacc tgtctgaaaa ccaaatgcag
     7201 gggaaatttc tccggcataa gcaattgggc caccttgttg cgggtttttt gttgccagtc
     7261 gggcatatac atacgccagc gacattgcac caataataga gataatccaa ccccagatag
     7321 caataccacc gatacttgct aggttcgcag gtaataatgc aataccgctc cccatcatat
     7381 taccggcaac aacaccggta caggcaaata gcccgatctt cttggcagaa ctcatgctct
     7441 tctcctaatt tcatttttga atttggagtc cgggtcatga tgtataacta tttcctgacc
     7501 agaccaaact ggcgctaaga atactcacga aaaaaggatt aatcctaaag attaggtgaa
     7561 ataacacaaa agtttctgta agtgagaact tgaggttttt tattaacaca tcaggatcgc
     7621 aagttgatat catgaaaaga taaacattca atgtttacaa tggattgcgg gatattctct
     7681 ggttaaattt atgtaataaa aattatgcgg caaataaatt gccgcaacat attataccaa
     7741 cagaaacata caaaaactca acaacaaata ttcccgagca taaatcaacc cgggagttac
     7801 ttattctgaa gcgagaaatt tgtcgagata aggtacaaca taaggaacag aagtctggaa
     7861 tataccattt tcaatccagt aaagggtgtt tgcccctggg cgtaaattaa aggcggtgag
     7921 atatgcatca gccgcttccc ggttcatccc cttcatttca taaaccttgc caagcaacac
     7981 ataatttagc caggacattt caagatcaat gccagtattt atcgcctggt aagactcatc
     8041 tgttttacct tttaccagag cactgaccgc ttttatttga tatataatgg ataggttatt
     8101 caattccggt agtgtaacaa tgttgtctat ttctgtgttc agtgctgcta attgtttttc
     8161 atctaaaggg tgttgagaat ggcgcacgat atcaactaat gctttttctg ctctcgcgta
     8221 ggtaaattct ggggatgatt gaacaatctc acctaataat tcactggcac ggttcaatga
     8281 tttatcatcg ccatgcagta aataatcatg tgcctgataa aaattagtta ataacgcacc
     8341 acgatgaggc aaaatttgct ggagcgtctc ctgcattcgt tgtggccacg gttggtttaa
     8401 tgcttttgat aaactctcca gtaaatcatt ttgaatcgcc agctgattac cgttagtgat
     8461 gacataacgt ttatccagca tggttgaacc atctgcattg tctaccaatt ttatcgacat
     8521 aaagcattgt tgagcacggt attggcgctg attaacaaag gcaatagata atgttttacc
     8581 ggaactgctc ggttcatcaa tgttgtagtt gattttgtca tgcaccataa aggtggagaa
     8641 ggtgttaagt gatgtcgcca ccaaatcacc cacgcctatc gcgtaagaga gctgatacgg
     8701 ggaactccag ctgttacaac ttttatttac catattaatg tcaatatcgc gtggattgag
     8761 caaaatacgc gatttgctca taggaagacg tgtatcaaga gttgaaaacg ctaccagtgc
     8821 tacacagata cctaacgaca acaggaaaaa aaaccatacc caaaaggtag tgaatcgttt
     8881 gcttttaact ggggattgtt caggtggcgt tgcggtgttt tgaatgttaa gactgtggga
     8941 gggagaatct gtggcaggaa ccgcctctgg tataggggga ggcgaagata gcattatttc
     9001 ctctccctct tcttcgctgt accagataac cggcaccatt aatttatagc cgcgctttgg
     9061 tacagtagcg atatagacag gactatcttc atcattatct tttaatgact tacgtagttc
     9121 tgagatactc tgcgtcacaa catgattggt gacaatactt ctcttccaga cattatcgat
     9181 aagttcatcc ctgctaagta cttcgccact gtgttgagca aagaaaacca gaagatcgat
     9241 taatctcggc tcaagggtaa gttgacgccc attgcggcta atttggttta tggacggagt
     9301 aacaagccat tcgccaacgc gaactacagg ttgttgcata atagaaactc attcgaaaag
     9361 ggaatgatgc aatgataatt gccacaacct atttttacca tctatagatg ggtttattta
     9421 catattattg gtgaatgtaa gacgttattt ttaccagcca taaacttctt gattacatag
     9481 tattacgaaa ggattttact gagaaccaga agtaatattc cttaccatca aaattcatca
     9541 tctttgccaa agaaaaatgt tcagaaaata atccatggaa aattgtccgg agcgcttact
     9601 attttaatgg attgttagtc tttgcatgag caagcggact gatacatttc tctttgttct
     9661 cattcagaaa atctcatcag tcggcgttca gaccggtgtt gtgctttatg gatgcgttac
     9721 gctcctgatg acgtcatttg acgttcaaca acatcacggg gccgcacgac atttcacgtc
     9781 agttagtgct atcgctcagg aacaaatttt cccgaattgg gatatgcccg caaattgctg
     9841 gtgatgtggg gagaatctgg ttgagttcgg gagaattgat ttggaggcag aacgcttaaa
     9901 ttgtggcgtc cagaaacgaa aaacggacct ccgtggaggt ccgttgatat gaatttggtg
     9961 ccggactcgg aatcgaagag tgcttctatc atgttgatat tagtttattt tgtttggttt
    10021 ggtgaaatat gtaccctctt ttgtacccgc agtccctttt gatatcgaaa gggaatcaaa
    10081 ctcggggtca ggctgagagc acagaaggaa agtgcctggc tattatatcg ttaattctgt
    10141 caatcagctc cagccttttg aaagtgatgt gtgcagatcc cttcttaaag tatttaatga
    10201 tgaacatctc atcttcatag cgtcctttac cctgaacgga ctgtatgtgg tcatccagat
    10261 taatggtgat attccctcgg ttgtcgggga tcggtttacc gctgagcaga tgcaacattc
    10321 tttccaggtc agccaggcga tctgcctgca ttccactgat aagatgaaat ccccactggt
    10381 cccatctcac cagattgttg acgataattt tactgccaaa tttacagggt gagttggttt
    10441 tgtaatccca gctcagccct ttgaagacgt tgatcactcc ccgttcaaac acttcatctt
    10501 tgttctggtg cagttgttca aatgtgctca gaatgttcgc ttcgctgatt tccggaaaat
    10561 tatcatattc cagtgacctg gacctgactg aggtggagca gataacgtcc gtattctcgt
    10621 tgaccacttc cggacgggag actgtgattt cagacataat cgatattcct ttttgcagat
    10681 aaatgaaaac gccagccgtg ttcaggctgg cgtcagtgga atgaagtctg gtgagtgaga
    10741 tgatgagtga gactcaccgg ttctggtgct gaagctgcag ttggcgcagg gtacggatat
    10801 cctctctggc ctgttcgata cagggcaaca gtgctgctga atccgtttta tcctcattgt
    10861 tgtcaaagat aattcccgat tcgcagtcga tattgtcctg cagccacgca atcagaatat
    10921 ccagtgcggt ttccgtggtt aatgatttca tgttgttcct ggttaccagt tgaaagtggg
    10981 taaacccggc aggcattcag cgctggcatc cagatgaatg agactgacgc cataacgtcg
    11041 ggtaagtgtg ataaccagac gacggaacgt ttttgacagc cccaggcgtt taagccgcag
    11101 aaccggatat gaccacgcat cggtacgtaa cagatatccg ctacctgtga aatgaatcca
    11161 ttctgattca ccaacgtcac tggtctgatg cgacagcgag tacagccagg cgttatcctt
    11221 ttccgtgata tgtgcggtac tgcagcgtat gccggcaagg gttgcaaacg gtggtagtgt
    11281 gcaggttgac tgttggtcgg attcctccac cacgcggagt gaataaccgt tttcagcgac
    11341 cttgttaatc agttcagtga gattaacacc atcgacgtca acgacaattc gccccatgtt
    11401 cagtgcctgc acattaattc tgttggcttc cagcatcagg gaaagtttca tcgtttctcc
    11461 ggatgcttac ccagggtaat gttatttacc gttctgtaat tgtcgcgggt catcaggccg
    11521 gtcgcccggc gagcccggag gatatcaata ctgtttatta actgagagcg gggacaagtg
    11581 ctgaatcccg gctggtcggt acgcaccagc gcgtattttt ccacgagaaa attcaccgca
    11641 tcacacagtg aaatgccagc ttcaatatgc tgctcaatca cacgttcatc ggcgaacggt
    11701 gtgtcattca gtgtaaggcc gtaatgctgg cccagtagtc gggagagcag agtctgccag
    11761 atggtgacgg gagactggca atgcgatgcc tcccgtacgt gtgtatcggg taatgtgttc
    11821 atactgaaga ttttcctgat atgcagagat gaaaaatgga aaagtggcgt ggtgaaagca
    11881 ccaggccgga gcagaaggtt attctggaga gttacttttt cgtttcgggc gtcggataaa
    11941 cagccatata aacgtagcca caactgccga gggtgtcggc ttcgcaggtc agcctttttg
    12001 catacagcgt gacggtatgc tgatggcggg gattcagttc accgctggtg agcatgagtt
    12061 ccagttgttt catcagcagc ggaaaggcct ggtccagatg gtacgcatcc gcgtcgctga
    12121 accggcctct gataccggcg cggtctgcaa ggtaatgcaa ccggttaccc tcctgcacca
    12181 gacgtgcccc aaaacagggc gtcacggtgc agggtagccc ccaccagggg tggtcgttgt
    12241 tatcgtcggg atgcgttgtc ccggggagtg tgtctgacac gataaaatcc tcacagaaaa
    12301 ttggttaaga atggtccggt ggtggcgata attctgctct tctgaatgcc cttccggact
    12361 cagttcaggg tgacgctcat cagccggaca tacggaccaa aactgtcctt acgacgttca
    12421 gcaaacacgg ccagcacacc gggaatatcc tgcacttcac gaccggtata cgcttcagtg
    12481 ctaccgtgcc agcggtattt accggtacag aacggaaaaa gacgggatgc aggatgctga
    12541 cggtgaatac gcatggcttc accacggggg atgattttca tgagaatagt cctcctgaat
    12601 gtgaaagtaa gaaacaactg agaaagagaa actgaagtga tatgaaggtg attgtgacgg
    12661 tgacgggtta aagcagaccg tgttccgcaa aggagaaaac ctggctgcca ccgactatca
    12721 gatggtccgg cacccggata tccaccagag ccagtgcctg taccagacgt tccgtgataa
    12781 ggcggtctgc cttgctgggt gtgacttcac cggacgggtg attgtgtgcc agcaccacgg
    12841 cagcggcatt gtggtacagg gcgcgtttaa tcacttcccg gggatggact tccgtacggt
    12901 tgatggtgcc ggtgaagagg gtttcaccgg caatcaactg attctggttg ttcagataca
    12961 gcacccggaa ctcttcacgc tccagtcccg ccatgttcag aatcagccat tcccgtgccg
    13021 cacgggtgga ggtgaaggcc acgccgggtt catgaagatg gcggtccagg gttttcaggg
    13081 ctcgctgaat gaggctgcgc tctctggttg tcatttctcc gggcagaaag gaaagctgct
    13141 gcattgtccc tgcctccatt cagtcgatga tgcgcataat ggcgctgcat tccggatgtt
    13201 gcagggcgta atcccgcagc cggtaataat ggaccgtcat ggcataacac tcagtacgac
    13261 aggcatgatg actgtacgtc atcagacagg cggcaatgcc ggcggcttcc ggactcattt
    13321 caacgcggtt accgttcatg gcattgaaca gtacccatgt ttcgtcatca tcttcatccg
    13381 gttcaggtgc cataaatgcc ccgccgttgt tcagggtata cagattccag ataccaccgc
    13441 agtagtcttc acacagacgg tccatccagc cgaagacacg gggctccagg ggcacccact
    13501 gtggaatgag gccaaagtgc tgcggccaga agctgatgcg ctgctcatca gggactatgg
    13561 tggcaaccag ttgagactgg ttattccctg atgcagtagt tacggaaaca gaaggtgtgg
    13621 tggagctatg cgaaaaggtt gtcatgagat gattccttat aaaaagtaaa tgaatggaag
    13681 aatgcctggg gattggtaca gacgagagtc agggctgcac tttcagggaa acagcattag
    13741 cgcatgctct ccagcag
//
LOCUS       DBANUU010000119        13474 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000119 DBANUU010000000
VERSION     DBANUU010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.120"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            40..3807
                     /locus_tag="ACIE68_003568"
     CDS             40..3807
                     /locus_tag="ACIE68_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFX5909827.1"
                     /translation="MPSVSRYRTWLAVPADEIEDLKKAHPPMNGHTPVIWDKEHKLWF
                     ARPGADLSRLDRWLPRPQDVSMNGSDPVTEFAQVLENAGLVLKELPVMDGKIHRVPTA
                     DDKKGQKSGAYRGFLDGRPAGWYRDYRSADDSPITWTFSGGEQTDPRARLHLKAHSMQ
                     RREDAERELKAQYNRQAAYARRYVNKWPQATAHEYLTRKGIQAAPGVRVNNKNELVIP
                     FSNRNGAIRSYQRIPVTGGKDARILKDSEKTGNWFALGTPRNGQPVLFAEGYATAASL
                     HEATGLPVLMTVDGGNMIAVAENARQKWTQSPFIFCADNDHAIRVNKGIVSATKAAEL
                     TGGTVIFPAFTDAEKAQGLTDFNDLDASRGRAAFQHVINAQLEHIGVSTPNSNTPEIR
                     EALVIGNLVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSLPSATQQEQPAASSSNI
                     ADETQSFTSHATENNGKDERHADNVQAQTESSVVQDSPAESPASPESTTASAPDEPAH
                     PAEPPEKVVSVATDKDWREFEAELSQPEKNESQQESGTSPEIPAPAAAPASPEDSPSS
                     ETMNESAEPSPSPDAVKEEMTVMTEPDDKQSTGISPDTPREEAVNHVTEDIPLQPEPF
                     LPESDGPEEYENYSAYQELMNNENPEHLQENSDMPSQPDTDHVQADESELQATTDTAE
                     PAVRDEPTPQQPVQSTSPSDNTSSFLDKARGFFTRKKTDSQAHENSDPTPSAETTTTP
                     PIPDSIVYAPERSDAPISLNMDEILKELDWEKRADRTVLYKLDGKPAFIDRVNRLEMV
                     NGASNDDRSVLAALAVATKFYGGVIELTGSDAFKQKAMLLIIEHNIDVRMKLPDQRAE
                     LEKLRKEMAVSKDAVVTHQPTPELNRNTPEQPAVPDPVQEKEATQSPASPVAPEASTV
                     STASAPSPAEPGKTADVAPDEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELK
                     NRSGSKLYWGEQLESLVKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNG
                     ISVSHDNPDKGQRIQAIPVETFMKVAAQISQGWPEEMKALRMPENVGSHLFIGEDRHP
                     VSAPQNANQVTEITSAAPDKLTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLY
                     PALATPSADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPL
                     FAAVYTPEKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            3825..4172
                     /locus_tag="ACIE68_003569"
     CDS             3825..4172
                     /locus_tag="ACIE68_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFX5909828.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPWKDYNL"
     gene            4169..4861
                     /locus_tag="ACIE68_003570"
     CDS             4169..4861
                     /locus_tag="ACIE68_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFX5909829.1"
                     /translation="MTENTEPSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTNQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFAGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            4872..5855
                     /gene="traN"
                     /locus_tag="ACIE68_003571"
     CDS             4872..5855
                     /gene="traN"
                     /locus_tag="ACIE68_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HFX5909830.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNA
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNENMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAILRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            5858..7147
                     /gene="traO"
                     /locus_tag="ACIE68_003572"
     CDS             5858..7147
                     /gene="traO"
                     /locus_tag="ACIE68_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFX5909831.1"
                     /translation="MSAEQDAGKSGKKLAALLGLSSIILFGGSYIAFSKLSGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDTP
                     EKQQQPAAKPENYAHRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATA
                     MWNKSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNPSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQTEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            7147..7851
                     /gene="traP"
                     /locus_tag="ACIE68_003573"
     CDS             7147..7851
                     /gene="traP"
                     /locus_tag="ACIE68_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HFX5909832.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAVVLL
                     AAIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSHLNGMMTQ
                     KSVTESSSTPHPAKKKTSSVLNGMKIMSMETGMAWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            7851..8378
                     /gene="traQ"
                     /locus_tag="ACIE68_003574"
     CDS             7851..8378
                     /gene="traQ"
                     /locus_tag="ACIE68_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFX5909833.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            8429..8833
                     /gene="traR"
                     /locus_tag="ACIE68_003575"
     CDS             8429..8833
                     /gene="traR"
                     /locus_tag="ACIE68_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HFX5909834.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKFLLLIPMMLLPKA
                     VLADGDLADMVRNVEQGAKTAQSSSLTIAQFIGVILFLGGLIGLKKVGKQGGMGLAGC
                     IVSIVIGALLVAGPEMMSRSQKQLGISTISIG"
     gene            8897..9085
                     /gene="traS"
                     /locus_tag="ACIE68_003576"
     CDS             8897..9085
                     /gene="traS"
                     /locus_tag="ACIE68_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HFX5909835.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            9069..9869
                     /gene="traT"
                     /locus_tag="ACIE68_003577"
     CDS             9069..9869
                     /gene="traT"
                     /locus_tag="ACIE68_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HFX5909836.1"
                     /translation="MQHSIKDLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLDKLKKRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTESLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            9959..13003
                     /gene="traU"
                     /locus_tag="ACIE68_003578"
     CDS             9959..13003
                     /gene="traU"
                     /locus_tag="ACIE68_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HFX5909837.1"
                     /translation="MNINRLIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGPGSLESLITSMSDSLNTAY
                     KNSGHKISFVFERDPDMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWTLSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERHSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMTLAATDEKEPVCIMTIMA
                     STWGKTREICTRNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAFEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPPERKDEAVGIILSND
                     PEYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEELVDSALQDSGLYEKHDATWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLLRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEELASELIRKQGYNL"
     gene            13003..>13474
                     /gene="traV"
                     /locus_tag="ACIE68_003579"
     CDS             13003..>13474
                     /gene="traV"
                     /locus_tag="ACIE68_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HFX5909838.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRVFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRK"
BASE COUNT         3578 a         3608 c         3425 g         2863 t
ORIGIN      
        1 gcggtggttt ttttccgtct tttttcagga gaagcactca tgccgtctgt atcccgctac
       61 cgcacctggc ttgccgtgcc tgcagatgaa atagaagatc tcaaaaaagc acatcccccg
      121 atgaacgggc acacgcccgt catctgggac aaggagcaca aactctggtt cgcccgcccg
      181 ggggcggacc tgtccaggct ggaccgctgg ctgccccggc cacaggacgt ctccatgaac
      241 ggcagtgatc cggtcaccga gtttgcccag gttctggaga atgccggact ggttctgaaa
      301 gaactgccgg tcatggacgg gaaaatccac cgtgtcccga ccgccgatga caaaaaagga
      361 caaaaaagtg gcgcatatcg cggattcctg gacggcagac ctgccggctg gtacagggat
      421 tatcgcagcg cggatgattc gccgatcacc tggaccttct ccggcggaga acagacagat
      481 ccccgcgcaa gactccacct gaaagcgcat tcaatgcaac gcagggagga cgcggagcgg
      541 gaactgaaag cacagtacaa ccgacaggcc gcgtatgccc gccgctatgt caacaaatgg
      601 ccgcaggcca cagcacatga atacctgacc cggaaaggca ttcaggctgc accgggggtg
      661 cgggtaaaca acaaaaatga actggttatt cccttcagta accgtaacgg cgcgatacgc
      721 tcttaccagc gtattccggt caccgggggg aaggatgccc gcatcctgaa agattcagag
      781 aaaaccggta actggtttgc gctgggcaca ccgcgcaacg gtcagccggt actgtttgcc
      841 gagggctatg ccactgcggc ctccctgcac gaagccacgg gccttccggt actgatgacg
      901 gtggatggag gcaacatgat tgccgttgcc gaaaatgccc ggcaaaagtg gacgcaaagc
      961 ccctttatct tctgtgccga caacgatcat gcaatccggg ttaacaaagg tatcgtcagt
     1021 gcaacaaaag ccgcagaact gacaggagga acggtcatct ttcctgcgtt caccgatgcc
     1081 gaaaaagcgc aggggctgac cgatttcaat gacctggacg caagccgggg acgggccgct
     1141 tttcagcatg tcatcaacgc ccagttagaa cacatcgggg tttccacccc caacagtaac
     1201 accccggaaa tccgcgaggc actggtgatc ggcaacctcg tttttacacc ggtccacact
     1261 gaggaaaaaa ccatgactcc gacagaatat cctgaaacat cacccgatac agggcacagc
     1321 catgaccagg gaccttctct gccgtccgcc acgcagcagg aacaaccagc tgcttccagc
     1381 agtaacattg ctgatgaaac acagtcattt accagtcacg ccactgaaaa taacggcaaa
     1441 gatgagcggc acgctgataa cgttcaggcg caaacggaaa gctctgtagt tcaggactcc
     1501 cctgcagaat caccggcgtc cccggaaagt acaacggcat ctgcccctga tgaacccgct
     1561 catccggcgg aacctccgga aaaagttgtc tcggtggcca cagataaaga ctggcgtgaa
     1621 tttgaagccg aactgtcaca accggaaaaa aacgagtcac aacaggaatc cggcacttca
     1681 cctgaaatac cggcccctgc tgccgccccc gcatcccctg aagacagtcc ctcatctgaa
     1741 accatgaatg aatcagccga gccatcacct tcccctgatg cagtcaaaga ggaaatgacc
     1801 gtgatgacgg agcctgatga caaacaaagc accggaatat ccccggatac tccccgggaa
     1861 gaggcggtca accatgtaac tgaagacatt ccccttcagc cagagccgtt ccttcctgag
     1921 tcagatggcc ccgaagaata cgaaaactac agcgcatatc aggagctgat gaacaatgaa
     1981 aatcctgagc atctgcagga aaacagtgat atgccgtcgc aaccggacac agaccacgtt
     2041 caggcagatg agtcagaact tcaggccaca acagatactg cagaaccggc tgtccgggat
     2101 gaacccactc cacagcaacc tgttcagagc acatcacctt ctgataatac ttcatcgttt
     2161 ctcgacaaag cgcgtggctt ttttacgcgt aaaaaaacgg attcacaggc acatgagaac
     2221 agtgatccca cgccctcagc ggaaaccacg acgactccac cgatacctga cagtattgtc
     2281 tacgccccgg aacgttctga tgcaccgata tcgctcaaca tggacgaaat tcttaaagag
     2341 cttgactggg aaaaacgagc agacagaacc gtcctgtaca aactggatgg caaacctgcc
     2401 ttcattgacc gggtaaaccg gctggaaatg gtcaacggtg ccagcaatga cgatcgcagc
     2461 gttctggccg cacttgctgt ggccaccaaa ttttatgggg gcgtcataga gctgacgggc
     2521 agtgatgcct ttaagcaaaa agcaatgctg ttgattatcg agcacaacat cgatgtccgg
     2581 atgaaattgc cagatcaacg ggctgaacta gaaaaattac gcaaggaaat ggctgtcagt
     2641 aaggatgcag ttgtcaccca tcagccaaca ccggaactta accggaatac cccagaacaa
     2701 ccggctgtcc ctgaccccgt tcaggaaaaa gaagccacac aatccccggc gtcccctgtt
     2761 gcccctgaag catcaaccgt cagcaccgcc tctgcaccat caccggctga accaggcaaa
     2821 acggcagatg tggcaccaga cgaagaacca ccaggaaaac ttcgcccggg agagagcgtg
     2881 actgccgtac tgcacaattt tggtcgtgct gaatatgcac ccggcaaagg agagagtttc
     2941 tttgttgagc ttaaaaaccg cagtggcagc aaactctact ggggggaaca actggaaagt
     3001 ctggtcaaaa accatcagaa aggtgacgtg gtgacactga cacttcagaa ccgcgaacaa
     3061 ttcatcctcc cgggagagca aaaagcacgt ttccggaata agtggtccat ggagtcagtt
     3121 accaacggga tctcggtatc acatgataat ccggataaag gtcagcgtat tcaggcgatt
     3181 ccggtcgaaa cctttatgaa agtggctgcg caaatcagcc aggggtggcc cgaagagatg
     3241 aaagcgttac gtatgccaga aaacgtcggc tcacatctgt ttatcggtga agaccgacat
     3301 ccggtctccg cgccacagaa cgcgaatcag gtaacagaaa taaccagcgc agcccctgac
     3361 aagctgacac ctgttctggg atcagtggat aaggatacgc gtgaactcaa cctgttactt
     3421 gtccagagtg ccgatgaaca tctgcagggt gtggtccggc tgaacggcac attgtatccg
     3481 gcactggcca ccccatcggc cgacaacagt cagttggtta tcaatgcgct gaccgacaaa
     3541 ggtttacgct ttgcaggtta tggtgaagcg gtaaaccacg atgctgacag cacgaatcgc
     3601 cccgcaccgg agcttatgca gtttcatctg aaaacccggg aagaaccgct ttttgctgct
     3661 gtctacacgc cggaaaaaca acctgatgcg ttgtaccgaa acctgggctt cgagcagtca
     3721 tggcagcagt ggtccaacag ccaaaagccg gaggacaggc aggaaaaaac tctgcatcag
     3781 gatctctccc acagccccgg ccgatgagcc ggtaaggaag ccatatgaac atgcataagt
     3841 ctctggttgc cgtgctcggc cttatcctga tttcatccac ggcaagtgct gcaacctgtc
     3901 gtgcgggtag cgcggcacag gccggtagta atacaggata tgagcgagca cgtgcagctg
     3961 cggatgcatg gtcacaacgt gaaaacgatg tttcctcatc cctgcagtcc tgtctgtcac
     4021 gaatcaaaaa aatcagcata aacctccccc agttccccag ccttgacgac attctgtcgc
     4081 agctggaaaa ccaggtctgc gacgcggtcg tggacaaggt gaatgaaaag ctgccgggga
     4141 atattgatcc ctggaaggac tacaacctat gaccgaaaat accgagccat cctcgccatc
     4201 acatccggca cctgacccgg aaatactgaa gagcacaatc agggctatga agcagtcaga
     4261 acagcgggca aataatgtcc ctgcgctgat aaaagcgctt ttatgtacgg gcacatgtct
     4321 tcttatcagt attactggta acgccattca gtactggcac tcaacgaatg tggagcgcga
     4381 atatttcgct acagacaatg gtcgtctggt caggctggca ccaacaaatc agccagcatg
     4441 gagtcagaat gacgcaatgg cctttggcag ccaggcactg gcgaccgcat tcaacctgga
     4501 ttttgtgcac tatcgttcac agatttcctc tctgtctccg cgtttttctg acgaaggttt
     4561 tgccggatat gtgaatgcat tacaggcatc taacattctg gagacgatca agaaagagaa
     4621 gatgaatctg accgccacga caggcgctgg cgttctggta cgacagggac aaatgtccga
     4681 cggcgtatgg ttctggacat ttcagtatcc tgtccgtatg cgtctggtcg gccagacaac
     4741 cagcaagcca gaacagtctt ttgttttcga aatcaccatc cagcgtgttg accccagact
     4801 gaaaccatcc ggtatggaaa tccggcagat gatctcccgt aacgccggac cgaactcctg
     4861 agataagtaa tatgcaatca cgttatttac tgagcacact gctcctggtg tgcagcgccg
     4921 ccacgtctgc agataacgca ggatggcaga acgcacgcac accacaaacg acaaattctg
     4981 ccagcgatca cgttcagaac gcctcccaaa acaccggagg tgttccggca acactggtaa
     5041 aaggtgagct gcctgccccc gggcaggcca gcccgctggt acaggatgcg gcaagacttg
     5101 attctgagct ctccgccgat gagattcgct cactacgcag tctcatggcg gataacgagc
     5161 gggccatcaa tgcgcccatc accagtgttg taccccgtat cagctctctg acagttaatc
     5221 tgtcccccgg tgccagcctt cctcttgttc gcacagccat gaacaacctg agcgtagtga
     5281 catttaccga tatcaacggt tcaccatggc cacaatccga tcctccttat aacgcggcac
     5341 caaaactctt tgatgtgcag tacaacgaga acatggtgac aatcacacca ttgcggccat
     5401 gggcatccgg aaacatttct gtctacctga aaggtctgtc ggtaccggtc atcctgaacg
     5461 taaccagtgg cgaaacagat acgccgtcat ccagtcagga aatggacagt cgtctggatc
     5521 tgcgaatacc acggcaggga ccaaccagcc cggttgtctc catcccgacg gataaaatcg
     5581 ccctgcatga cgcaacatta caggcatttc tggatggtat tcctccccgg gacccttcag
     5641 ttaagaggct gaaattcaca ggtaatgtcc cggatacaac gatctggcaa cacggcgatg
     5701 accttctggt tcgttcccgg gcgatattgc gcgatgaatt tgaacagacc ttgtcctctg
     5761 ccgacggaac tcatctctgg aagcttcccg tcaccccact actgactttc tctgtgaatg
     5821 gccagtccgt tcacgtgaca ccagaactgg agtaatcatg agtgctgaac aggatgccgg
     5881 taaatccgga aagaaactgg ctgccctgct tgggctgagc agcatcatct tatttggcgg
     5941 cagctatatc gcgttttcca aactatcagg gagtaacagc gatatgcagt cagccgtgaa
     6001 cataaacagt gctgcaagtg gcggtacgcg cagcgttacc gaaacgcctc actatcgtga
     6061 actgttaaga gcggataatg aacgaggggc tgcggcggca gcccgcaata atcagacgtt
     6121 cattgccagt cttccgcagg ggcttgatat tccggacact ccggaaaagc aacaacagcc
     6181 tgcagcaaaa ccggaaaact atgctcatcg ccaggcttcc ggcacaccac aggaggatcg
     6241 agcagccagt gaaaaacgta tggagcggct gcaaaagctg attgtaagaa taaaagatca
     6301 gcatcctgcc ggtagtacac caaccatagc aacggctatg tggaataaat ctccggcaga
     6361 aacaacaggg cagaatggta cgcagcaatt cgcattacag aatgccagcc tgtccacgcc
     6421 ggtagcagaa aaagggatac aactgatacc tgctctgacc cgtataccag catatattga
     6481 tacggcagtg gattctgata acccgtcatc caaggttatc gcaaccattc ccgctggccc
     6541 ctgggcaggg gcaacgctgt tcagtccggg agtcaaactg gtgggtaacg gcgtggagat
     6601 ccatttcgac cggatgagct ggaacggcat ggaccttaag gtcaatgcat atgctcaacg
     6661 ggaagacaac ctgatgtcat ccgtggccag caatgtgaat acacgctggt ttaaacatat
     6721 cattctgccg tcagtgttag gaggtgtggg cagtattggc acactttata aagatgccaa
     6781 cacgcaggtt attcagggta actatggcac ggttaccgga cgcgtgggaa tgcccagtgg
     6841 tgaagctgtt gcgggcgtta ttgccggtgg tatggcggaa agaggaagtc agatattaac
     6901 ccgccaggca gaaagtgagc cctataaaca ggtagaagtg tatcagcatg aagtcgtatc
     6961 aattctgttt gttgacccgg taatgacaaa cgacgccagg tcatcttccc tttccagtgg
     7021 catttcccct tcagttaatc gtacatcaca gacggaacaa cgctcgcagg cacgaatgca
     7081 gacagccatg gagcaaagaa aagctgtgat gcaacgtcgg tatgatgaac aaccggagac
     7141 accataatga aacctgaaac agaaatagat gaaaccggca gcttcggtga acccgaagag
     7201 aaacccgccc ctttctggaa acgttccgtc tgggggatat ctgtagcaac atgggggcta
     7261 tgtgcagtcg tgttgctcgc agccatatgg tatctgttcc tgagagcacc atcggaaaca
     7321 ggcatgcctc cgtttaatga tgcggatgct ggcgtacaga cctggcaaac cacacaagaa
     7381 tcatcacctt ccgttcagtc cactgagacc atgactgtcc ggacaggaga agatatgagc
     7441 cagcttgccc gggatgtaaa aacagagctg gataaccgcg atgaaaaaat tcaggcaaca
     7501 ctgaatatgc ttcacgacag cattaacaag ctgggcgaag ctataaaaaa ggatgaagaa
     7561 tacgcacagg aaacgcgccg tcagctggat gatatccgga gccacctgaa cggaatgatg
     7621 acacagaaaa gcgtcaccga atcctcttcc acaccgcatc cagcaaagaa aaaaacgtcg
     7681 tctgtactta acggaatgaa aatcatgtcg atggaaactg gcatggcctg gattcgctgg
     7741 cagggtagta cctgggctgt cagagaaggt cagactctgg gcaatgtggt gatccagcgg
     7801 attgatccga cgacccggac catcatcacc agtgcaggga cgttacgctg atgaacatgg
     7861 atgccctgac cgccattgag aactttgcca gcagtatttt cagtgccgga atggatttcc
     7921 tgtttacctg gggagaattc atcggggtga tcagcatgat taccctgttt gcccgcgccc
     7981 gttcagccgg tccggtaaaa atgtctcccg gaaaatttat tgcggggatg ttaaccagct
     8041 gcatgctcgt atcactcccg gcaatgatta acgccggagg cgtacaaatg ggattcaggg
     8101 ctgactcatt tggcccgatt gcatatgtac aacctcagac gtttggcgct gctgctggcg
     8161 cagcaaatgc agttctgtcg ctggccaagc tggccggtgt tggatttgta atgaacggga
     8221 tatccatctg gcgcaaggcc ggactcgatg gacataccgc actgagtgcc agtgaatccg
     8281 tcagtaaggg aaacgtcaaa tttattgccg gcgtcctgct ggtctttatc gatcgtgtcc
     8341 tcaatgccct gctggcaagc attggaatcg tcttctgatt tttttcacct tcatataaac
     8401 cactgatata tccatttaag gagaaactat gtcacgtatt cgtgtcgtag cggcccgtct
     8461 gtacgccgca tttatccacg cacaactgtt tgtaatgtca tgccggagca aactgactaa
     8521 gttcctgctg ctcattccca tgatgttgct gcccaaagcc gttcttgccg atggagatct
     8581 tgccgatatg gttcgcaatg tcgaacaagg tgccaaaaca gcacagtcat catcactgac
     8641 tattgcgcag ttcatcggcg taattctgtt tctcgggggc ctgattggcc tgaaaaaagt
     8701 tgggaaacaa ggggggatgg gactggctgg ttgcattgta tctatcgtca ttggggcgct
     8761 acttgtcgcc ggacctgaaa tgatgagtcg cagccagaaa cagctgggga tttccacaat
     8821 ttccattggc taatcttgtc cagggcactg gcatttatcc ggtgcccttc ctgttcggca
     8881 atacaaggag gcacatatgt ctgcctggag cccggagcac ataacgttac gacgaacaga
     8941 aaacggcaat gtgattgccc acgacagtcg ggacgatcag gaattcattt ttgcagaatg
     9001 ctgttccgat gaaatcgcaa acatcattct ggaagccgtg aaacgttaca ccagtacgga
     9061 gcctgctcat gcaacacagc attaaagact tgtggttgta tccctttcca gaaattgatg
     9121 tggttcatac acaggagcca ctgttaccgg aaccggaatt aacaacgccc ggtcgatgta
     9181 tctgctgccg gcaaaacgtc aggcaccgtt tcagactgga tgactcatgg ccactcagac
     9241 agcttaccga cacaatcagt gacacacgag ttcgtcttaa taaggccaca gaacatctgg
     9301 ataagttaaa aaagcgtggt gaaccagtcg caaccggcga aaaggaaaag tacaacacgg
     9361 cagtaaaagc cgctgaacgg gcgcttgagc aggcccgtct gtctgccaga cgtctttcac
     9421 tccgccatgt acagaaagct gaaatcacca gtacagagtc gttgtcagag aaagagcagg
     9481 aactgtttca tgaagacggc ccgccttaca gcttatgtgc tttctgccac gcctggcatt
     9541 cactgaatgg ttatgccgca gcacagggcg ttatggtgtg gctacccgac cttcatccat
     9601 ccacggtcgt ggcccttaac cgtcgttcat tacaggaagt tttcagtaat gataagttcc
     9661 gggtccggcg gggacgggaa gcgctctctg cactgatgca aaacagactg gcggttgagg
     9721 ataaattccg cagtttccgc ccggccgatt tcgctgacgt ttttcgtcgc tatccacctt
     9781 ccggtcgttc gcccctgcgt gaaaaaatga acgggattgc cctgattctg acaccagatt
     9841 cattcatcaa aaaagaatac gttgattgat ttttcttcat gccactccag cgcggggtgg
     9901 cattaaaaaa atcaaactgc acaaaccgcc ctcctgtaag tccggagttc cctcacctat
     9961 gaacataaac cgtttaatct tcactatcga agactgtctg aacacgctat cccgtttctc
    10021 tgtcgcaagc agttttgttg agtattgcga tttacgtact gtaattggtc tggacagaca
    10081 ggatcgtgaa cgtcgcccat ggctgaattc gccctatatt gcagccacaa agcgtggaga
    10141 atatctctct gtctttgaag tcagcggtgc tttcagggaa atggatgaag catccgacca
    10201 gactggccca ggttcactgg aatcgctgat caccagcatg agtgacagcc tgaatacggc
    10261 atataaaaac agcggtcata aaatcagctt cgtttttgag cgtgatcctg acatggggaa
    10321 agaagaaatc gaagacatgg tcgccccaca aaagcgctca ctggctaata caggtatcca
    10381 gttacaggat gtggtcgatg agaaagtcac aacactgtcc ccatggctgg tccgggagcg
    10441 ttgctggctg gcgatctgga gtggacccga ccttatcagc aacagcgatc gcaccgccca
    10501 tgatgagctt gtcagaagac tggctgaacg ggtaccgaaa gcacgctttg cccagtcccc
    10561 ctggcagtgg accttatctg cactgaaaat ccgccacgaa gcttttcttg ataatgtgga
    10621 acaggcactg agacattcat ctgatggcct gattctccgg ctactggata tacacgaggt
    10681 cgggcgtgaa atacgtcgtc agacagaacg gcacagcaca ccgcgtaact ggcaacccca
    10741 cctgcctgaa gatgcccagc cagcgggata tcgctggact gacgatgaat ccgtactaca
    10801 tgctccttca ttgcatttgc agttattcaa cactcaggtg accacacagg gcaacctggt
    10861 tcaggcgggt ggactctggc acggaatggt atcgattact ttacctccgc aaaatctgca
    10921 gacgtttaac gaactggtcc gtgccgtccc ccgcgcagtc ccctggcgca tcagaatgga
    10981 tctgatgccc ggcgggatga aggctcttaa tctgaagaaa acgcttctga cttacagcag
    11041 cttcatttct gccgttcgtc caatgtatga atccgttatg accctggcgg caacggatga
    11101 aaaagaacct gtctgcataa tgaccatcat ggcctccacg tggggaaaaa cacgtgaaat
    11161 atgcacacga aaccaggcca ttctgaaatc ggccattgaa ggctggggcg tatgtggcac
    11221 cacgacaaca tttggcgatc cccgacgcgc ctgggtgaat accattctcg cggcatccgg
    11281 aggctccggc ccggttcccc tgtatccccc gttgtcacat gccatctcgc ttttcccgct
    11341 taacagggca ggctctgtct ggcgaggcaa agggaatctg atgttacata cagaagacgg
    11401 ttccgcattt gaggttggtc tggcgtcatc ccaacaaaat aaacatacag aactggcacc
    11461 aggcgatccg ggactgggta aatcagttct gattaatacg ttgtcggaaa tacagatatc
    11521 atctgctcag aaaaacctgc ccttcattgc ctatatcgat aagggataca gtgctcaggg
    11581 actggttcag cttattcgcg acagtcttcc tccggaaaga aaagacgaag ctgtcggtat
    11641 tattctgagc aatgatccgg aatacacccg taaccttttt gacgtgatgt acggcgctaa
    11701 aaagcctatt acgccggaaa agaatttcat gtcatctgtg ctgtgtgctc tctgtgtcga
    11761 taccggtacc ggtcaaccct gtaaccccgg agacacccga caaattatca accagcttat
    11821 cgagctggct tttaaagagt acggtgaaaa taatccgcga ctttatcgcg cctcaacgga
    11881 agaactggtg gattcagcac tgcaggactc gggactgtat gaaaaacatg atgcaacctg
    11941 gtgggccaga tcaacctggt tcgaagtccg ggatatgctc cacaatgccg ggtatatcat
    12001 ggcggctcag cgggcacatt atcaggcaat gccccagctt cccgaagtgt cctcgatgct
    12061 gggccatacc agtctcaggg atgttttcgg tactgttcag cgtgatggtt caaacgaact
    12121 tctgctggac tacatccgcc gcgctctcga gcagggacat aacgactacc ccatgatatc
    12181 cgggtatacc cggtttatga tcaacccgga aacccgggtc atcgctgtcg atctgaataa
    12241 tgttgccggt gacaaaacgc ctgcaggcag gcttaaaacc ggcatcatgt acctgcttgc
    12301 aggtcagatc gccggtggtg acttcaccct gccacaatac agggatgaag tgttaaagca
    12361 gcttccacgg gaataccatg aaatcgctct taaacgtatt aaccaacttg atcaggaggt
    12421 taagacaaaa gtttatgatg aactgcataa cgcccggggc attgacttca tctgggaaaa
    12481 tctggatacc caggagcgtg agcaacgcaa gtttgctatc cgcacggttc tgagtacgca
    12541 gtatctgagg gactacccgg aaagcgttct taagtcagca aatactctct ggttactgcg
    12601 ctacaaaccc gaagatatcc ccgtactgag ggataacttc aacgttcctg aatttatgct
    12661 gaaacgcttc ctgaaaatgc cggaaggccc ggcaccagat ggtagtggtg ttcctgttct
    12721 gggagtattc agggtaaaaa gtggcacgct tgcgagaata ctcaaattta ccgtcggccc
    12781 actggagcta tgggcgctga actcatcgcc aaaagacagt gccctgcgta aaacactgac
    12841 aaataagtta ggttcagttc gggcacgtaa gattctggcc gaaaacttcc ccagaggttc
    12901 tgcaacgtca ttaattgagc accgggcggg ccaacataac agtgacaatg ttattgaaga
    12961 actggcctcg gagctcatcc gaaaacaggg atacaatctc tgatgtgtac aattcatatc
    13021 accccacgct gccggagacc tgccagaggg caggtatttc ttaaaaaact tgaggtaaaa
    13081 ggtgacagtt tctcattcac tctgccgtca cgtgtcttca ggttacatcc ggcccccaat
    13141 cccagagtga tatacgcctc ggatgccaga acacagggaa aggtcacagg catgcggctg
    13201 ttgttgccgt ccagcacaga tgagctgata acaacggcaa tatggcaggt ttctgtcccg
    13261 gatgaagcac cattcgacgt tcagcatgtc atccagtgga tactcccttc accgctggct
    13321 tcgctgatgt ccgatgatac ctggttatgg ccatccgttc ccggaacgcc ggcactgagt
    13381 gccggtgact ggcctgttat ggatactgac ccttccacct tacggcgcgt cagacgtcgt
    13441 tctttcagca tcatcgataa ccggaacagg aaag
//
LOCUS       DBANUU010000120        13395 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000120 DBANUU010000000
VERSION     DBANUU010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.121"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(177..629)
                     /gene="yafP"
                     /locus_tag="ACIE68_003580"
     CDS             complement(177..629)
                     /gene="yafP"
                     /locus_tag="ACIE68_003580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFX5909839.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVMMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIERHIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQIVKQQHVECRGAWFTNFYMRYKPQH"
     gene            complement(626..1681)
                     /gene="dinB"
                     /locus_tag="ACIE68_003581"
     CDS             complement(626..1681)
                     /gene="dinB"
                     /locus_tag="ACIE68_003581"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFX5909840.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELHLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(1752..2537)
                     /gene="lafU"
                     /locus_tag="ACIE68_003582"
     CDS             complement(1752..2537)
                     /gene="lafU"
                     /locus_tag="ACIE68_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFX5909841.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFSGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            2509..4221
                     /locus_tag="ACIE68_003583"
                     /pseudo
     CDS             2509..4221
                     /locus_tag="ACIE68_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4539..5036)
                     /gene="rayT"
                     /locus_tag="ACIE68_003584"
     CDS             complement(4539..5036)
                     /gene="rayT"
                     /locus_tag="ACIE68_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFX5909842.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRHAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5212..5961)
                     /gene="yafL"
                     /locus_tag="ACIE68_003585"
     CDS             complement(5212..5961)
                     /gene="yafL"
                     /locus_tag="ACIE68_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFX5909843.1"
                     /translation="MSSFLLGRFLHPGVFSLCVLLPLFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GENIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            6262..7002
                     /gene="dpaA"
                     /locus_tag="ACIE68_003586"
     CDS             6262..7002
                     /gene="dpaA"
                     /locus_tag="ACIE68_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFX5909844.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(6973..7740)
                     /gene="yafJ"
                     /locus_tag="ACIE68_003587"
     CDS             complement(6973..7740)
                     /gene="yafJ"
                     /locus_tag="ACIE68_003587"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFX5909845.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(7946..8524)
                     /gene="lpcA"
                     /locus_tag="ACIE68_003588"
     CDS             complement(7946..8524)
                     /gene="lpcA"
                     /locus_tag="ACIE68_003588"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFX5909846.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            8764..11208
                     /gene="fadE"
                     /locus_tag="ACIE68_003589"
     CDS             8764..11208
                     /gene="fadE"
                     /locus_tag="ACIE68_003589"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFX5909847.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLCSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(11251..11697)
                     /gene="ivy"
                     /locus_tag="ACIE68_003590"
     CDS             complement(11251..11697)
                     /gene="ivy"
                     /locus_tag="ACIE68_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFX5909848.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            11878..12648
                     /gene="yafV"
                     /locus_tag="ACIE68_003591"
     CDS             11878..12648
                     /gene="yafV"
                     /locus_tag="ACIE68_003591"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFX5909849.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQNDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWQDADEFRLR"
     gene            complement(12690..>13395)
                     /locus_tag="ACIE68_003592"
     CDS             complement(12690..>13395)
                     /locus_tag="ACIE68_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFX5909850.1"
                     /translation="TDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQG
                     GDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAMSEKSHGREEIRLHIVCDVPDE
                     LIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWH
                     VENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAA
                     MDRNYLASVLAESGLS"
BASE COUNT         3122 a         3456 c         3546 g         3271 t
ORIGIN      
        1 aatattaaaa atcaggaaat ggcagacgaa tccagggcaa gaaaaaaaga attaatcctg
       61 gaaaggtctg caaagagtca ttgataaagg atttgtcatg atttacctcc ttgcagtttg
      121 tggaataaga aaacgcgcac ataccaggcg ggcgttattt tcattgcaag ctggatttaa
      181 tgttgcggtt tatatcgcat ataaaaatta gtaaaccacg ctccccggca ttcaacgtgc
      241 tgctgcttaa ctatctgaaa accataacgt tcaaaaaagg gttttgcggt tatgctggcg
      301 tccaccgtaa gttcggattc agacttaatc aaaggtttta acaaggcgct ggcaaccccg
      361 cggcgggtgt attcagggtc aacaaataac atatcgatat gacgttcaat gcgggaaata
      421 aaaccaaccg gttgtgcatt aatgactgca acccgcactt gtgatttcgc gagtttctcc
      481 ttccagcgag attcgtcaat ctgcgcccag gcggcaattt gttgtggtga ataatgctga
      541 ctggcggtca tcataaccgc tctaatgaaa atagcgcata gttgctgaaa atcgccaggc
      601 tgatagtttc ttatttgtat gttattcata atcccagcac cagttgtctt tccatttgag
      661 ggtcaagcaa cgtcacatgc agccccacca gtcgcacacc gcgcccgccg cggcgttcat
      721 cccaggtttt acgcgcggtg gcgattagat cagctttatt cagccgcggc cagacgtgct
      781 cctgggtggt ttgctgaaaa tcgtcgaact ttaatttcac cccctggcga gcaatcagta
      841 aatcaggttt tacctttgcc agacggcgtt caagttccgg atacagccgc tcgataatcg
      901 cttcacattc agaccagtga tgaatatctt ccgccatcgt gcgttccaca ccgacggatt
      961 ttcgcaaccg ttcgctgtta acgtcgcgtt cgtcaatccc ctgactacgc tcccacaaaa
     1021 tgcggccaaa tttgccaaag cgtttaagca gaatcaccag atcacacttt tgcacatcac
     1081 cgcaggtccg cagccccatc gcttccagtt ttgccgccga gactttgccg acgccgggga
     1141 tttttgccaa tggtaaggtt tgcagaaatg ccggaacttc tgccggagta atcacaaact
     1201 ggccgttggg tttattcatg tcggaggcga ttttggcgag aaacttgacc ggcgcaacgc
     1261 ccgcagacgc cgtcaggtgc agctcgttga aaatcgtctg gcggatttcc tgggcgatga
     1321 gggtcgcaga accgtggcaa tggacgctat cggtgacatc gagataagcc tcatccagtg
     1381 acaacggttc aatgcgcgag gtgtagcgcg agaagatttc acggatatga tttgaggctt
     1441 ctttgtaggc gtcaaagcgc cccggaagca aggtgagatg tgggcataat ttgagcgcca
     1501 tcccagtagg catagcgcta cgtacgccaa atttacgcgc gggataattg gcggtgctga
     1561 tcaccccccg acgttcgcgg ctgccgccaa tagcaatagg gatatcgcgc agggcgggat
     1621 tgtcgcgcat ctccactgcg gcgaaaaagc agtccatatc cacatgaatg attttacgca
     1681 ttgctcacct ctcaacactg gtaaagtata cagtgatttc agggtttgag aaatgcgtaa
     1741 agattcagca tttagcgcat tcgctgcgaa agcacctgct gcttatccag cttttgcacc
     1801 agcggctgca ccactttatc cccatgctga ccaaagtatt gatacagcgt atcggacgca
     1861 cttttggtca gcaccataat ctcaatacgc cgattgcccg cgctttgcgg atttttggca
     1921 tccagcagca tctggtccgc cattgcgctt acctgcatca ctttatcttc cggcattccg
     1981 gcctcttcca gcacccgacg agctgaaagc gcgcgatcgc ccgaaaggtt ccagttgttg
     2041 tagatattgt ttttgtaggc catcgcatcg gtatgcccgg taataataat tttgttatcg
     2101 agcgagtcga acactggcgc gagctccacc agcaaagttt taaagaacgg cataatctgc
     2161 gcgctgccgc gttcgaacat attgcggttc tggtcatctt taatcagcac gcgcaatccc
     2221 tggggaacaa tctccatctc cagattcgct tccatatgcg catcacgggc aatggtgttg
     2281 atactggttg ccagctcgcc aagctcggtg gcggatttct tcttcagcag cgctgttttc
     2341 tcgttgacgt ctcgcgcttt tttctccgtc tcttccggca ccgccacggt ggcaggtttt
     2401 ggcggatgag acgggctgat tttatttaac ggcgacaatc cgccgccact gaaaatcgac
     2461 tgcccgtgca gcgcggcgat aatgctttca cgctcggatt tactgacgct gttgacaatc
     2521 aactttatcg tcgtcaccaa aggggccgag cgtatttccg aggtttctgc ccgctttacc
     2581 ctggacgcga tgcccggcaa acagatggcg attgacgccg accttaacgc cgggttgatc
     2641 aaccaggcgc aggcgcaaac ccggcgtaaa gatgtcgcca gcgaggccga tttctacggc
     2701 gcgatggacg gggcgtcaaa gtttgtgcgc ggggatgcca tcgccgggat gatgattctg
     2761 gcaatcaacc tgatcggcgg cgtctgtatc gggatcttca aatacaacct gagcgccgat
     2821 gctgccttcc agcagtatgt gctgatgacc atcggcgacg ggctggtggc gcagatccct
     2881 tccctgctgc tctccaccgc ggcggcgatt attgtcaccc gcatcagcga caacggcgat
     2941 atcacccatg acgtgcgcca ccagctgctg gcaagcccgt cggtgctcta caccgctacc
     3001 gggatcatgt tcgtgctggc agtggtgccg gggatgccgc acctgccgtt tttgctgttc
     3061 agcgccctgc ttggctttac cggctggcgg atgagcaaac gcccgcaggc ggcggaggcg
     3121 gaagaaaaaa gcctcgaaac gctgacccgc accatcaccg aaaccagcga gcaacaggtc
     3181 agttgggaga cgataccgct gatcgagcct atcagcttaa gcctcggcta caagctggtg
     3241 gcgctggtgg ataaagcgca gggcaacccg ctcacccagc ggattcgcgg cgtgcggcag
     3301 gtgatatccg acggtaacgg cgtgctgctg ccggagatcc gtattcggga aaacttccgc
     3361 ctcaagccca gccagtacgc tattttcatc aacggcatta aggctgatga agcggatatt
     3421 ccggcggata aactgatggc cctaccctcc agcgaaacct acggcgaaat tgacggcgtg
     3481 ctggggaacg acccggcgta cgggatgccg gtgacctgga tccagcccgc gcagaaagcg
     3541 aaggcgctga atatggggta tcaggtgatc gacagcgcca gcgtgattgc cacgcatgtg
     3601 aataagattg tgcgtagcta tattcctgat ttatttaact atgacgatat tacgcagttg
     3661 cataaccgtt tgtcgtcgat ggcaccgcgc ctggcggaag atttaagcgc ggcgctgaat
     3721 tacagccagt tgctgaaagt gtaccgcgcg ctgctgaccg aaggcgtttc cctgcgcgat
     3781 atcgtcacca ttgccaccgt gctggtcgcc agtagcgcgg tgaccaaaga tcatattctg
     3841 ctggcagctg atgtgcgcct ggcgctgcgg cgaagcatta cccatccgtt cgttcgcaag
     3901 caggagctga cggtgtatac gctgaataat gagctggaaa atctgctgac caacgtggtg
     3961 aatcaggcgc aacagggcgg gaaagtgatg ctcgacagcg tgccggtcga tccgaatatg
     4021 ctcaaccagt tccagagtac gatgccgcag gtaaaagagc agatgaaagc ggcggggaaa
     4081 gatccggtgc tgctggtgcc gcctcagctg cgccctttgc tggcgcgtta tgcaaggttg
     4141 tttgcgccgg ggctgcatgt gctgtcgtat aacgaagtgc cggacgagct ggagttgaag
     4201 ataatggggg cgttgatgta agtatccggc attggtgcac aactgccgga tgcggcgtga
     4261 acgccttatc cgtcctactg ttcaggcacg attcgtaggc cggataagcg caagcgcatc
     4321 cggcaccagc gcacaaccgc cggatgcggc gtaagcgcct tatccggcct accgatccag
     4381 cacaggtttg taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcataaccg
     4441 ccggatgcgg cgtaaacgcc ttatccggcc taccgatccg gcacaggttt gtaggcatga
     4501 taagacgcgc cagcgtcgca tcaggcatct gcgcataatt ataaaatgat acgctcgcca
     4561 gcatttatat ccgttacgtc ccccgcccaa tcgatgggat ataaccctcg cgcgacatcg
     4621 cgatggaacg ttgagaatgg ccaatcactc acttgcttta cccaaccatg ctttactgga
     4681 tttatataaa tataatcaac atgatgccta taatctttgg tattgcggat ggcgtgctcc
     4741 caaaaacgtg gttgccagat atttttcaat ccacaagcat gggtaaattg ctttttaatt
     4801 tcccgccagc gcgaggaaaa atcatcatcg ccttcaggta atgtccagat acagtgcata
     4861 tgctctggca aaacgaccca ggcgttgatt tcaaaaggcc tgtctcgctt aactttaata
     4921 atggcgtgac ggagcatctg gtactgggtg gtcaagagtt ggcttcgacg attgcgtaaa
     4981 ttcaccgtga aaaaccaggt tccccccttg atgtaataac gacgatattc agacatggcg
     5041 actccttttt ctcaacggta agaagaaaag gaaaggtcgg taatgaggtg atggaataac
     5101 aggaaagcgg ctcgcaaaaa cgcacagatt tgtaggcatg ataagacgcg tcagcgtcgc
     5161 atcaggcatc cgtgcacaac cgccggatgc ggcgtaaacg ccttatccgg cctacaaaat
     5221 cgtctcttcc gtcaagatcc gccgcgcgcc caaaaaatga tcctgccaga aaggttcggc
     5281 taaccggctt acccgaatgt tttccccggt gcgcggcgac tcgataaact gcccatcgcc
     5341 cagatacacc cccatatgat cggcaatttc acggctgtgg atatggaaaa acagcaaatc
     5401 tccccggcgc aggtcgttat tcgccacaat cgttgcccga tgatagtgat acatctcatt
     5461 ggccgtgcgc gggagcttcg cctcaaggat cttgttgtag gcataaaaaa ccaacccgct
     5521 acagtcgaag ccttgatcgg gccgcgtgcc gccccagacg taaggcttgc caagctgttg
     5581 ctccagacgg tgaatcgcca cttcggtgat gttatgcaga tggtcgctgc ttaaaaagtg
     5641 attgttctcc gccagcgcct gccagcgtcg gtcgctcgcc ttgagcggcg caggaaacca
     5701 ttctggatgc tgttttatct gctcccggtt gtgctggcgt agcgcccttc tgctttcggc
     5761 attgccttcc acaatatagc tggcctgttt tttcagatgg gtttggtact gttttaataa
     5821 acgcgcacgg ttttgcatcc gctggcgggc ggcgtagctg aaagagatat gtgatgtggt
     5881 ggcgctggcg aataaaggga gcaaaacgca taacgaaaac acgcctggat gcagaaatct
     5941 gccgagcaag aacgaagaca tgaaagacat aaaccggctg aatgaaggtg agaattttaa
     6001 aagaccggag gagtaaacgg gattattaat tgcttatttg catattacta ttgaggtttc
     6061 ataatcttag gaaataaatg atacatgcgc ttttcggact attgaaaact gcccctgtgc
     6121 taaatttgta aaccctgcgc cgacaggctt gcgtaaaact cttttggtga taatgtgagc
     6181 cttccaaatc cagactattc cgatttggga tccattttgc cgtcctggcg tgtgtaaccg
     6241 ttttatcaag gaataagcag tatgcgtaaa atcgcattaa ttcttgcgat gttgttgatc
     6301 ccgtgcgttt cgtttgccgg tttgctgggc agcagtagct cgaccacgcc agtgagcaaa
     6361 gagtataagc agcagttgat gggatcccct gtctacatcc agatcttcaa ggaagaacgt
     6421 acgctcgatc tctacgtcaa aatgggcgag caatatcagc tgctcgacag ttataaaatc
     6481 tgtaaatatt ccggcggctt agggccaaaa cagcgtcagg gcgatttcaa aagcccggaa
     6541 gggttttata gcgtccagcg taatcagtta aaaccagaca gccgttacta caaagcgatt
     6601 aatattggtt tccccaatgc ctatgaccgt gcgcatggtt atgaagggaa atacctgatg
     6661 atccacggcg attgtgtttc catcggctgc tacgcgatga ccaatcaggg cattgacgag
     6721 attttccagt tcgttactgg cgcactggta tttggtcagc cgagcgtgca agtgagcatt
     6781 tacccgttcc gcatgaccga cgctaatatg aagcgccaca aatattccaa ctttaaggac
     6841 ttctgggagc aactgaagcc gggttacgac tactttgagc aaacccgtaa gccaccgacc
     6901 gtttctgtgg tcaatggtcg ttacgtggtc agcaagccgt taagccacga agttgtgcaa
     6961 ccacagctgg catcaaacta cacgctcccc gaggcaaaat aagcgccatt cgcctggcat
     7021 aattttttgc caggtttcat tgcccgtcag cggctgtgtc gcaatcaccg tgaccacatc
     7081 attcggtgtg gtctgcgagc tgaagtcgat ttccacatcc tgatccagca acgttgccac
     7141 gccaaacggc gcgcggcggg tgatccagtg taaattagtc gagcaatacg ccattacata
     7201 gcgcccgtca gaaagcagca tgttgaaaac gcccttctgc cgcagttcat ccgccagtga
     7261 ggcgatatat ttaaataccg ccgccatgtt gcccggtgtg cgcgggtaac gctgcgttaa
     7321 tttatgcagg agccagcaaa aggctttttc gctgtcggtt tcgcctaccg ggcggaagtt
     7381 gccggtttcc agtgatttgt agcccgtcag ttgtccgtta tgggcataag tccagttacg
     7441 cccccataac tcgcgggtaa atgggtgagt attttccagc gccacctcgc cccgattagc
     7501 ctggcgaata tgagccacca ccgaacagga ttttatcggg tagtcctgga caagtttggc
     7561 gatgggggaa ttaaagctgg gttgtggatc tttaaatgtg cgacagcctt taccttcgta
     7621 aaaggtaatg ccccagccat ctttatgtgg cccggttcca ccaccacgct gtacaagccc
     7681 ggtgaaacta aagcagatat cggtagggac gttggcgctc atcccgagca attcgcacat
     7741 gggtaacctc cacaacagcg ggagcgcatg gcccccggcg aagattgatt taagaatttt
     7801 caggtcggat gcggcgtgaa cgcattattc ggcctacggt tcgcgtacag atttgtaggc
     7861 atgataagac gcgtcagcgt cgcatcaggc atcggtgcac aattgccgga tgcggcgtaa
     7921 acgtcttatc cggcctacgc cagacttact taaccatctc tttttcaatc aactggatca
     7981 ggatatggat cactttaatg tgaatctcct gaatgcggtc ggcataacca aagtgcggta
     8041 cgcgaatttc gatatccgcc gtgccagcca ttttgccgcc gtctttaccg gtcagggtga
     8101 tcactttcat tcccttctca cgcgccgctg cgatcgcttt gatcacgttt gcagagttac
     8161 cggaggtgga gatccccagc agtacatcgc cttcgcgacc taccgcttca acgtaacggg
     8221 agaaaatatc attgaaaccg aaatcgttac cgacgcatga aatatgacta acgtcagaaa
     8281 tagcaatcgc cgggtagccc ggacggtttt cacggtagcg accggtcaac tcttcggcaa
     8341 agtgcatagc gtcgcaatgg gaaccgccgt tgccgcagga aagcactttg ccaccggctt
     8401 taaagctgtc tgctaacagg accgccgcgc gctgaatggc gtgaatattg gcgtcatctt
     8461 ttaaaaagtt agccagcgtt tccgccgctt cgttcagttc gttacgaata agatcctggt
     8521 acatgaggat atccttcagc ataaatgtaa tagacaaaat gcagtgtacc ggataccgcc
     8581 aaaagcgaga agtacgggca ggtgctatga ccaggacttt ttgacctgaa gtgcggataa
     8641 aaacagcaac aatgtgagct ttgttgtaat tatattgtaa acacattgct aaatgttttt
     8701 acatccacta caaccatatc atcacaagtg gtcagacctc ctacaagtaa ggggcttttc
     8761 gttatgatga ttttgagtat tctcgctacg gttgtcctgc tcggcgcgtt gttctatcac
     8821 cgcgtgagct tatttatcag cagtctgatt ttgctcgcct ggacagccgc cctcggcgtt
     8881 gctggtctgt ggtcggcgtg ggtactggtg cctctggcca ttatcctcgt gccatttaac
     8941 tttgcgccta tgcgtaagtc gatgatttcc gcgccggtat ttcgcggttt ccgtaaggtg
     9001 atgccgccga tgtcgcgcac tgagaaagaa gcgattgatg cgggcaccac ctggtgggag
     9061 ggcgacttgt tccagggcaa gccggactgg aaaaagctgc ataactatcc gcagccgcgc
     9121 ctgaccgcag aagagcaagc gtttctcgac ggtccggtag aagaagcctg ccggatggcg
     9181 aatgatttcc agatcaccca tgagctggct gatttgccgc cggagttgtg ggcgtacctt
     9241 aaagagcatc gtttcttcgc gatgatcatc aaaaaagagt acggcgggct ggagttctcg
     9301 gcttatgccc agtctcgcgt gctgcaaaaa ctctccggcg tgagcgggat cctggcgatt
     9361 accgtcggcg tgccgaactc attaggcccg ggcgagctgc tgcaacatta cggcactgac
     9421 gagcagaaaa atcactatct gccgcgtctg gcgcgtggtc aggagatccc ttgctttgca
     9481 ctgaccagcc cggaagcggg ttccgatgca ggcgctatcc cggataccgg gattgtctgc
     9541 atgggcgaat ggcagggcca gcaggtgctg gggatgcgtc tgacctggaa caaacgctac
     9601 attacgctgg caccgattgc gaccgtgctt gggctggcgt ttaaactctc cgacccggag
     9661 aaattgcttg gtggcgcaga agatttaggt attacctgtg cgctgatccc aaccaccacg
     9721 ccgggcgtgg aaattggtcg tcgccacttc ccgctgaacg tgccgttcca gaatggaccg
     9781 acgcgcggta aagatgtctt cgtaccgatc gattacatta tcggcgggcc gaaaatggcc
     9841 gggcaaggct ggcggatgct ggtggagtgc ctctcggtag gccgcggcat caccctgcct
     9901 tccaactcaa ccggcggcgt gaaatcagta gcgctggcaa cgggtgcgta tgcgcacatt
     9961 cgtcgtcagt tcaaaatctc tattggtaag atggaaggga ttgaagagcc gctggcgcgt
    10021 attgccggga acgcttatgt aatggatgct gcggcatcgc tgattaccta cggcattatg
    10081 ctcggcgaaa aacctgccgt gctgtcggct atcgttaagt atcactgtac tcaccgcggg
    10141 cagcagtcga ttattgatgc gatggatatc actggtggca aaggcattat gctcgggcaa
    10201 agcaacttcc tggcgcgtgc ttaccagggc gcaccgattg ccatcaccgt tgaaggggct
    10261 aacattctga cccgcagcat gatgatcttc ggacaaggag cgattcgttg ccatccgtac
    10321 gtgctggaag agatggaagc ggcgaagaac aatgacgtca acgcgttcga taaactgttg
    10381 ttcaaacata tcggtcacgt cggtagcaac aaagttcgca gcttctggct gggcctgacg
    10441 cgcggtttaa ccagcagcac gccaaccggc gatgccacta aacgctacta tcagcacctg
    10501 aaccgcctga gcgccaacct cgccctgctt tctgatgtct cgatggcagt gctgggcggc
    10561 agcctgaaac gtcgtgagcg tatctcggca cgtctgggtg atattttaag ccagctttac
    10621 ctcgcctctg ccgttctgaa gcgttatgac gacgaaggcc gtaatgaagc cgacctgccg
    10681 ctggtgcact ggggcgtaca ggatgcgctg tatcaggcag aacaggcgat ggacgattta
    10741 ctgcaaaact tcccgaaccg cgtggttgcc gggctgctga atgtggtgat cttcccgacc
    10801 ggacgtcatt atctggcacc gtctgacaag ctggatcata aggtggcgaa gattttacaa
    10861 gtgccgaacg ccactcgctc acgtattggt cggggtcagt acctgacgcc gagcgagcat
    10921 aatccggttg gtttactgga agaggcgttg gtggatgtga ttgccgccga cccgattcat
    10981 cagcggatct gtaaagagct gggtaaaaat ctgccattta cccgtctgga tgaactggcg
    11041 cacaacgcgc tggcgaaggg gctgattgat aaagatgaag ccgctattct ggtgaaagcg
    11101 gaagaaagcc gtctgtgcag tattaacgtt gatgactttg atccggaaga gctggcgacg
    11161 aagccggtaa agttgccgga gaaagtgcgg aaagttgaag ccgcgtaatt gaataacgga
    11221 gccgaaaggc tccgtttctt tatccgctaa ttatttaaaa ttaaagccat ccggatggtt
    11281 ttccaggctg ccggtcaacg ccgcgaacag caccgtttta ccatcaatcg aaagcgcatc
    11341 gttcacattc agccaggtga gtttctcttg cgacgttttc tcatcaatag tcgagaacag
    11401 ccccgtcatc tgattagatt tctcggacca catcacagcg atacgttgcg agccacagtc
    11461 atgcggtttg cacgcgctca tcacctgata cgtctcatct cccaacgtta cggtttgtgc
    11521 gggagtataa gtaccgcctt tcatcaccca tgcaggtagc ttatgccctt gcaccatctg
    11581 attaaacgca gctttggtgg tttcgccctt tgccaggctg ctaatggtta aatcatcctg
    11641 cgccattgca ctggtggcga tgaccagagc ggcgactgtc gttattgcct taaacatcat
    11701 tcctcccgag cttatcctgc ccatgttatt aacctcctaa agcctggcga taacacgata
    11761 atttgcaaat cagatatttc caatatcagc atttgttatc aatttattct gcgctaaggc
    11821 aactgcctga taaggtgtta aagttataaa acccttcgca taaaggagtt gtacaccgtg
    11881 cctggtttga agattacgct tttgcagcaa ccactggtgt ggatggatgg ccctgccaac
    11941 ctgcgtcatt ttgatcgtca actggaaggt attaccgggc gcgatgtgat cgttctaccg
    12001 gagatgttta ccagcggctt tgccatggaa gcggcagctt cgtcgctagc acaaaatgac
    12061 gtagtgaact ggatgacagc taaagcgcag cagtgcaatg cgctgattgc aggcagtgtt
    12121 gcattacaaa cggagtctgg ttcggttaac cgctttttgc tggttgagcc gggcggcacg
    12181 gtacattttt atgataagcg tcatctgttc cgcatggcag atgagcatct gcattataaa
    12241 gcgggcaatg cgcgagtgat tgtggaatgg cgcggctggc gtattttgcc gctggtgtgc
    12301 tacgacttac gttttcctgt gtggtcgcgc aatctcaacg attatgacct cgccatctac
    12361 gttgccaact ggcctgctcc gcgctctctg cactggcagg cattgctgac ggcccgcgcg
    12421 attgagaatc aggcgtatgt ggcgggatgc aatcgcgtcg gcagcgatgg caacggctgc
    12481 cattatcgcg gtgacagccg ggtgattaac ccgcagggcg agattatcgc tactgccgac
    12541 gcgcatcagg caacgcgcat tgatgcggag ctgtcgatgg tggcattgcg ggaatataga
    12601 gaaaagtttc cggcatggca ggatgcggat gagtttaggt tgcggtgagt gggggttgtg
    12661 tttaaggctg ggggagagtc ggggtaagac tacgaaagcc cgctctccgc aaggactgac
    12721 gccagatagt ttctgtccat agctgctttt cgcatcttac gtcttaaccc tgccttgaat
    12781 accttatcat tcgtcaaaat attaatagcg atgtgccgta tccctgaaaa taattctgcg
    12841 gcgtttcctc ttcttatttt gcagtcgtct tcattcatta ccacgtccag acgccagtgc
    12901 agcttattct ccacgtgcca gtggtttcgg attgctgtgg cgaacttctc tgcggttaaa
    12961 tcagcagaac tgatataata tctgaccgtc atttcgggct ctttcttttg ttctgctatt
    13021 attgaccgaa aggagactgc cacgcataat ttcttcagtc ctttccattc aaacgtgaaa
    13081 tcaataagtt catcagggac atcgcaaaca atatgaagac ggatttcttc tctgccgtga
    13141 ctcttttcac tcattgcgta actgtcatgc tctggattat ttaattcttt cagcggaaat
    13201 ttttcctcaa aggctttatt tagccgcccc tggtttcctt ttaccgcgaa taaataatca
    13261 cctccctgtt tttgtatctt ctctgcaata tctttctggc aacccatcgc atcagttgtg
    13321 atgatttttc ctttaatatc cagcatgtta agaagttctg ggatagctgt aatctcatta
    13381 gatttctcat ccgtc
//
LOCUS       DBANUU010000121        13254 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000121 DBANUU010000000
VERSION     DBANUU010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.122"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            159..235
                     /locus_tag="ACIE68_003593"
     tRNA            159..235
                     /locus_tag="ACIE68_003593"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:193..195,aa:Arg,seq:acg)
     gene            516..1082
                     /gene="yqaB"
                     /locus_tag="ACIE68_003594"
     CDS             516..1082
                     /gene="yqaB"
                     /locus_tag="ACIE68_003594"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFX5909851.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            1079..1507
                     /locus_tag="ACIE68_003595"
     CDS             1079..1507
                     /locus_tag="ACIE68_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFX5909852.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1580..3136
                     /gene="gshA"
                     /locus_tag="ACIE68_003596"
     CDS             1580..3136
                     /gene="gshA"
                     /locus_tag="ACIE68_003596"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFX5909853.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            3286..3801
                     /gene="luxS"
                     /locus_tag="ACIE68_003597"
     CDS             3286..3801
                     /gene="luxS"
                     /locus_tag="ACIE68_003597"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFX5909854.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGKGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3865..5403)
                     /gene="emrB"
                     /locus_tag="ACIE68_003598"
     CDS             complement(3865..5403)
                     /gene="emrB"
                     /locus_tag="ACIE68_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFX5909855.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5420..6592)
                     /gene="emrA"
                     /locus_tag="ACIE68_003599"
     CDS             complement(5420..6592)
                     /gene="emrA"
                     /locus_tag="ACIE68_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFX5909856.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6719..7249)
                     /gene="emrR"
                     /locus_tag="ACIE68_003600"
     CDS             complement(6719..7249)
                     /gene="emrR"
                     /locus_tag="ACIE68_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFX5909857.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(7340..7675)
                     /gene="ygaH"
                     /locus_tag="ACIE68_003601"
     CDS             complement(7340..7675)
                     /gene="ygaH"
                     /locus_tag="ACIE68_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFX5909858.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALTYG
                     LVWKVMAII"
     gene            complement(7665..8402)
                     /gene="ygaZ"
                     /locus_tag="ACIE68_003602"
     CDS             complement(7665..8402)
                     /gene="ygaZ"
                     /locus_tag="ACIE68_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFX5909859.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALTGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8526..9710)
                     /locus_tag="ACIE68_003603"
     CDS             complement(8526..9710)
                     /locus_tag="ACIE68_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5909860.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(10002..10994)
                     /gene="proX"
                     /locus_tag="ACIE68_003604"
     CDS             complement(10002..10994)
                     /gene="proX"
                     /locus_tag="ACIE68_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFX5909861.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(11052..12116)
                     /gene="proW"
                     /locus_tag="ACIE68_003605"
     CDS             complement(11052..12116)
                     /gene="proW"
                     /locus_tag="ACIE68_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFX5909862.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(12109..>13254)
                     /gene="proV"
                     /locus_tag="ACIE68_003606"
     CDS             complement(12109..>13254)
                     /gene="proV"
                     /locus_tag="ACIE68_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFX5909863.1"
                     /translation="RAFKYIEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLS
                     GSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHM
                     TVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARAL
                     AINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAI
                     MQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFG
                     PRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALIDAPLAVDAQ
                     TPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
BASE COUNT         3301 a         3422 c         3497 g         3034 t
ORIGIN      
        1 agcggaatta accgcaggca ccagggatga taacgttgct ttagcaacgg cccgaagggc
       61 gagccgcaag gcgagtaatc ctcccggatg caccatctct tacttgatat ggctttagta
      121 gcgatatcaa tatcagcagt aaaataaatt ttcccgatgc atccgtagct cagctggata
      181 gagtactcgg ctacgaaccg agcggtcgga ggttcgaatc ctcccggatg caccatattc
      241 tccgtaacct tcagcaatga aggtatcgaa gagatagcgg tattaaccgc aggcaccagg
      301 gaggataacg ttgcgtcagc aacggcccga agggcgaacg ccagtgagta atcctcccgg
      361 atgcaccatc ttctcccaaa aatttccttc aaaaatatct catttctaaa taactacttt
      421 atccatttcc tgaattttct tctccttcgc catcagcgac aaaatcaagc aattacgcta
      481 aagtagcgtc atatttttcg acttgcgaga agaccatgta cgagcgttat gcaggtttaa
      541 tttttgatat ggatggcaca atcctggata cggagcctac gcaccgtaaa gcgtggcgcg
      601 aagtattagg gcactacggt cttcagtacg atgttcaggc aatgattgcg cttaatggat
      661 cgcccacctg gcgtattgct caggcaatta ttgagctgaa tcaggccgat ctcgacccgc
      721 atgcgttagc gcgtgaaaaa acagaagcag taagaagtat gctgctggat agtgtcgagc
      781 cgcttcctct tgttgaagtg gtgaaaagtt ggcatggtcg tcgcccaatg gctgtaggaa
      841 cggggagtga aagcgccatc gctgaggcat tgctggcgca cctgggatta cgccgttatt
      901 ttgacgccgt cgtcgctgct gatcacgtca aacaccataa acccgcgcca gacacatttt
      961 tgttgtgcgc gcagcgtatg ggcgtgcaac cgacgcagtg tgtggtcttt gaagatgccg
     1021 atttcggtat tcaggcggcc cgtgcagcag gcatggacgc cgtggatgtt cgcttgctgt
     1081 gagtgaagcg ttatcgcttt tctcgttgtt tgccagtagt tttctcagcg ctacactatt
     1141 acccggcaac tccgaagtcg tgctggtggc aatgttgctt tccgggatca gttatccctg
     1201 ggttttagtt ttaacagcaa caatgggtaa tagccttgga gggttaacta acgttatcct
     1261 tgggcgtttc tttccattgc gtaaaacatc gcgctggcaa gagaaagcta ccggctggct
     1321 gaaacgctat ggtgcagtca cgctattatt aagctggatg cccgtggttg gcgatttact
     1381 gtgtctgtta gcgggatgga tgcgcatctc ctgggggcca gtaatctttt ttttgtgcct
     1441 tggtaaagcg ttacgctatg ttgcagttgc agcagcgacc gttcagggca tgatgtggtg
     1501 gcactaattg taggcctgca catatggtca ccattacagt tatgctaatt aaaacgattt
     1561 tgacaggcgg gaggtcaatt tgatcccgga cgtatcacag gcgctggcct ggctggaaaa
     1621 acatcctcag gcgttaaagg ggatacagcg tgggctggag cgcgaaactt tgcgtgttaa
     1681 tgctgatggc acactggcaa caacaggtca tcctgaagca ttaggttccg cactgacgca
     1741 caaatggatt actaccgatt ttgcggaagc attgctggaa ttcattacac cagtggatgg
     1801 tgatattgaa catatgctga cctttatgcg cgatctgcat cgttatacgg cgcgcaatat
     1861 gggtgatgag cggatgtggc cgttaagtat gccatgctac atcgcagaag gtcaggacat
     1921 cgaactggca cagtacggca cttctaacac cggacgcttt aaaacgctgt atcgtgaagg
     1981 gctgaaaaat cgctacggcg cgctgatgca aaccatttcc ggcgtgcact acaatttctc
     2041 tttgccaatg gcattctggc aagcgaagtg cggtgatatc tcgggcgctg atgccaaaga
     2101 gaaaatttct gcgggctatt tccgcgttat ccgcaattac tatcgtttcg gttgggtcat
     2161 tccttatctg tttggtgcat ctccggcgat ttgttcttct ttcctgcaag gaaaaccaac
     2221 gtcgctgccg tttgagaaaa ccgagtgcgg tatgtattac ctgccgtatg cgacctctct
     2281 tcgtttgagc gatctcggct ataccaataa atcgcaaagc aatcttggta ttaccttcaa
     2341 cgatctttac gagtacgtag cgggccttaa acaggcaatc aaaacgccat cggaagagta
     2401 cgcgaagatt ggtattgaga aagacggtaa gaggctgcaa atcaatagca acgtgctgca
     2461 gattgaaaac gaactgtatg cgccgattcg tccaaaacgc gttacccgca gcggcgagtc
     2521 accttctgat gcgctgttac gtggcggcat tgaatatatt gaagtgcgtt cgctggacat
     2581 caacccgttc tcgccgattg gtgtagatga acagcaggtg cgattcctcg acctgtttat
     2641 ggtctggtgt gcgctggctg atgcaccgga aatgagcagt agcgaacttg cctgtacgcg
     2701 cgttaactgg aaccgggtga tccttgaagg tcgcaaaccg ggcctgacgc tgggtatcgg
     2761 ctgcgaaacc gcgcagttcc cgttactgca ggtgggtaaa gatctgttcc gcgatctgaa
     2821 acgcgtcgcg caaacgctgg atagcattaa cggcggcgaa gcgtatcaga aagtgtgtga
     2881 tgaactggtt gcctgcttcg ataatcccga tctgactttc tctgcccgta tcttaaggtc
     2941 tatgattgat actggtattg gcggaacagg caaagcgttt gcagaagcct accgtaatct
     3001 gctgcgtgaa gagccgctgg aaattctgcg cgaagaggat tttgtagccg agcgcgaggc
     3061 atctgaacgc cgtcagcagg aaatggaaac cgctgatacc gaaccgtttg cggtgtggct
     3121 ggaaaaacac gcctgacaga aaagaaaaag gccactcgtg agtggccaaa atttcatctc
     3181 tgaattcagg gatgatgata acaaatgcgc gtctttcata tactcagact cgcctgggaa
     3241 gaaagagttc agaaaatttt aaaaaaaatt accggaggtg gctaaatgcc gttgttagat
     3301 agcttcacag tcgatcatac ccggatggaa gcgcctgcag ttcgggtggc gaaaacaatg
     3361 aacaccccgc atggcgacgc aatcaccgtg ttcgatctgc gcttctgcgt gccgaacaaa
     3421 gaagtgatgc cagaaagagg gatccatacc ctggagcacc tgtttgctgg ttttatgcgt
     3481 aaccatctta acggtaaggg tgtagagatt atcgatatct cgccaatggg ctgccgcacc
     3541 ggtttttata tgagtctgat tggtacgcca gatgagcagc gtgttgctga tgcctggaaa
     3601 gcggcaatgg aagacgtgct gaaagtgcag gatcagaatc agatcccgga actgaatgtc
     3661 taccagtgtg gcacttacca gatgcactcg ttgcaggaag cgcaggatat tgcgcgtagc
     3721 attctggaac gtgacgtacg catcaacagc aacgaagaac tggcactgcc gaaagagaag
     3781 ttgcaggaac tgcacatcta gtcagtaaac tatcttcaca attaattgaa aaaagccagt
     3841 tcaaatgaac tggcttagtt gtacttagtg cgcaccgcct ccgccgccac ctgcgccaaa
     3901 tggcggttta gcaaaccaca ccagccccag cagtacgagg aatatcccgg ctgacatcca
     3961 gaagatctca ttggcggaaa taatcagccc ctgattggtg atctgctggg caatccagcc
     4021 tgatgcctgc tgttgcgtca tcccaagccc ttccagttga ctgtacatcg cctgggcatt
     4081 cgggttgaac gggtttaccg actcagtcaa ctgcgcatgg tgcatcgact cgcggttggt
     4141 ccacatggtc gtggttatcg acgtgccgat agaccccgcc agcgttcgcg taaagttaga
     4201 gaggctcgat gccgccgcca gtcgttccgg tggcaaacca gacagcgtaa tggtggtcag
     4261 cggcataaag aagcaggcca ccgcaaaccc ctggataaac tgcggccagg ccgacgcgcc
     4321 aaaatccata cctggttcaa aggtataggc acgccagtag aagcagacgg catacataat
     4381 aaagctgaag gttaccagcc gccgcatatc cagtttatgc gcgaagcggc cgataatcgg
     4441 cgacaggatc accggaataa tccctaccgg cgcagaggcc aaacctgccc aggtcgccgt
     4501 gtaaccgtag acctcctgca acaactgcgg cagcagaaca atagcgccga agtagagcat
     4561 atacgcgagg ctgatacaca agcagccgat ggtgaagttg cgcgacttaa acaacgacag
     4621 atcgactatc gggttatcgt cggtcagctc ccagacaatc aggaagcaga tagccaccac
     4681 cgccaccacg gtaagaatga taatttcctg tgatgaaaac cagtccagct ctttaccgcg
     4741 gtcgagcata atctgcaggc tgccgatacc aataaccagc agtgccagcc ccacggcatc
     4801 aatccgccgc cgttcggtgc gggtttcacg tccgcgcaga gtttgcagtg tcatcaacac
     4861 caccgccacg ccaatcggca cgttgatgaa gaatatccag ccccagtggt aattatcgct
     4921 gatataaccg ccgaggatcg ggccgcaaat tggcgcgaca atcaccgtca tcgaccacaa
     4981 cgccagcgcg atcgagcgtt tggctggcgg gtagttattc agcaatagac tttgcgaaag
     5041 cgggatcaac ggcccggcga caatcccctg aatcacgcgg aagaagatca gcatattcag
     5101 gctgctggag acaccacacg cccacgacgc aatagcaaag gcgatggtgg accaaaggaa
     5161 cagtttcact tccccgacgc gctttgccag ccagccggta agcgggatcg agatggcatt
     5221 cgccaccccg aaagaagtga ttacccacgt tccctggctg agcgatgagc ccagattccc
     5281 ggcgatagtg gggatcgcca cgttagcaat ggtggagtcc agcacctgca tgaatgtcgc
     5341 cagtgacagc gcaatcgtca taatgaccag ttgcgcgcct tccagcggtt tttgctgttg
     5401 catcacacgc acctctggat tagccagcgt tagcttttac gatatcgtcg atcagtttat
     5461 tgacaggtgc caggctgatt tcacgcgcgg tacttaccgc gaccggtgtt gaacgtactt
     5521 tatttgccag tacctgaccg tcacggttag tggtattgac gctcaccagc gtggacaaac
     5581 caatgcgcag cgggtattgc tccagctgtt tctggtccag ttcgatacgc acaggtagac
     5641 gctgaacgac tttgatccag ttaccggtcg cattttgcgc cggaagcagt gagaacgcgc
     5701 tacctgtgcc catatccaga ccaaccactt taccggtgta tttcacatca tcgccgtaaa
     5761 tatcggtggt gatggtgacc ggctgaccga tgcgcatatt ggcaatctgc gtctctttaa
     5821 agttggcatc cacccacata ttggtggctg gaacgaccgc catcagcggc gtcgttgggc
     5881 taatttgcgc acccggttgc acggcacgac gggaaacata accagtcatc ggactgacaa
     5941 tacgggtacg ctccagcgcc agccaggcgt tacgtacttc ggtggcagcc tgttgcacgg
     6001 caggctgatc ttccagttta gtccccagaa tcatcgcctg attggcattg tattgttgaa
     6061 tagcgacgtc cagttgcgcc tgggcactgg tgacggcgtc gcgggcgtgt tgcagctctt
     6121 cgcgaccaat caggttggca ttgcccagcg gcacacggcg gttgtagtcg ctttgtgctt
     6181 tcgcgagggc gattttctgc acctcaatat tcgcctgcaa ctgcttgctg ttaatcatca
     6241 gctggtgggt ttggcgaacg ctggaagcca gtgcagtttt ggctttttca aacgcctggc
     6301 gagcatctgt cgggtcgaga gtgaccagaa catcgccttc ttttacaaaa tcggtgttat
     6361 cggcccagac tttcgtcacg ctgccagaca cctgagacat aatttgaatc tgattccctg
     6421 ccacgtatgc gtcatcggtt tcttcgaaat gacgcagtac caaaaaccaa taaatcccta
     6481 tcgctacggc aataattata aagagcaagg tgagaaggag gagcagacgc ttacgtttgc
     6541 cgctcttctt taccggttgc tgcggggttt gagtctccgc atttgcgctc atattgttct
     6601 ccacgatctt cttaactcat cggctgagcc gacctgcttg ccaaaaaggc cagcatgttg
     6661 cgatgctggc cagtcatttt ttcttttata aatctggatt tttgagcgag atgacgcgtt
     6721 agctcatcgc ttcgagaacc acaccgtctt gttccatctg gtcgagacgg gagagcaatt
     6781 tgcgggtgat ttgctcgagc tgatcttttt ctgttgtgct gagcgcggac cagagttgat
     6841 gcaggcagtt atgctgcggt ggtaaaacct cgcgcaaaaa ctcgtgacct ttttccgtta
     6901 attgcagatg caggcagcgg cgatcgttat cgctttcacg acgttcgatc caaccgcgtt
     6961 tttccagttc atcggcaata cgcgtcgcgt tggtacggga tgatccaaga gcacaactta
     7021 attcagaagg ctgaatactg tggttttcct gagactccag cgtaatcaac gccataaaca
     7081 acgtctcgtt aatcccctga gccttcagca ttttattgcg gttctccagc agcttgcttt
     7141 gcatgtgcat gcaaagacga gtcagaagga tctcctgata aggaaaatct tcgtggcggc
     7201 tggcgcgaaa ttttagcatt tgttcaatgg gcgtaaacga actatccatt tgggtatgac
     7261 ctcattaatt tcagccgata tagtaacgac agtgacaaat aaagtaaatg tattgtttta
     7321 gaaaaatgat tcttgtggat tatataatcg ccatcacttt ccagacgagc ccgtaggtca
     7381 gcgcactaag cagtgttggg atgataatgc tgcgtgtttt atagaaactg gcacccagta
     7441 ccgcgaagcc gaccagcgtg ggcacgaaac ggcgtgtatc gtgcatcact tctggtgcgg
     7501 tagagacaac cagcagagcg catatcgagg cgatgccaat ggtgtcgagc aaaataccta
     7561 ccgcgccacg tttggttggg cgggcattac ccacacgcag gcgcagcggc aaatagcgga
     7621 agcaataatt cgccgcgcca accagtaacc caagcagcag aacctcatag ctcatcgggc
     7681 gctccttgcc agaatgcctg gattaacgca gtgaggcagc cacagacaat gcctgccaga
     7741 atggcgacgg gaatagaaaa tagcgttacg cctgtaaggg caccaactaa cgctgcggta
     7801 acgcaaagag attgtttgcg ctggaaagag gcgagcagga aactcataaa gagtgccgga
     7861 agcataaaac ctaatgctgc ttcaacggcg ggataacctt gcagcaagcc gctgccggag
     7921 aatgccccta ttaccgtacc aaatacccac gatgaccatg aactgaaggc aatgccgatc
     7981 atccagttct cgctccagcg gcgattattg cgtaccagtt ttgcggttgc ggcggcaaaa
     8041 acctcatccg tcaggccaaa cgcccacagg gcggttttcg atttttgcag acgctgaata
     8101 atacggctac gcagtgacgg gccatacaac acatggcgaa catccattgc catgacggtc
     8161 agtgcagcaa cccacaaact actcccggct gccagcattg cggtaatgac gaactggctc
     8221 gcgcctgcat aaatgatgca ggagaaaaaa acgctttcga gaggagagaa tcccagacgg
     8281 gtcgcattca gaccgaacgc aaaggccacc ggaatataac taataacaat cggtaaactg
     8341 tctttgcatc cttccatgaa ggtcgccgaa ccaggagcag gctgtggagt agggctttcc
     8401 atagagtgta cgcttaacga ttgttaatat tataaataga ctgaatgaat atcttaacct
     8461 tatcagactg atgggcttct taacaccctt ataagtgtaa agccacgaaa acggttgctg
     8521 attgcttaat ttgcggcttc ctgacgatga aaacctcgcc accagaccag taagttaacc
     8581 agggcaatcg tcgcgccagc cagacaaacg ccagcccaac cgccatgttg ccaggctgag
     8641 gctgaaatta gcgaaccggc ggcaccgcca ataaagtagc tagtcatgta acctgcggtc
     8701 aggcgattac gcgcatcagg atgtatccga taaattaccg tctggttagt gatatgcacg
     8761 ccctgcacgg tgagatccag caccaggatt ccgataatca acgccagtac ggaagtgtga
     8821 ccaaaccaga tcgccagcca tgaaagtaat agcagcagca gaccgaaagt tgtggtgtgg
     8881 tgcgatttgc ccttatcggc aaaaccgccc gccggacgag cgcccaacgc tccggcagct
     8941 cccgcaagtc caaacagacc aattacacca tcgctgtagt taaaaggtgg agcggcaagc
     9001 aaaaaggcca ttgaggtcca gagaatgctg aaattggcaa aggtcaggca gcccagcaac
     9061 gcgcgggtac gcagaatttt atcgctgata aacatactga aaacggaacc caacaactgt
     9121 gggtagttga ggtgggtttc tgatttcatt tgtggcagac cacgccataa tgccagcgcc
     9181 atcagtgcca ttaacaccga agcaacccaa aagacggtgc gccagccgcc gagattcgcc
     9241 agcaatccgg caactgtccg tgccagcaag atccccaaca gcagaccgct cataatagtg
     9301 ccaaccactt tgccgcgttt gtccggtgaa gccagcgtcg ctgccagcgg aaccagaatt
     9361 tgtgccacga cagagaataa accggttaat gccgtaccga ggatcatcat cgccagcgac
     9421 tgactgctgg cagtgattaa catgccgcct gccgccagta aggtcatcga gacaatcagg
     9481 cggcggcgtt caaacatatc accgagggga acaagaaaca gcagacctgc tgcatagccc
     9541 aattgcgcgg cggtaacaat aaagcctgcc gaactggcgg aaagggaaaa gttacgcgcg
     9601 atggtgtcga gcaatggctg agcgtaatag ttactggcga ccgccagacc ggtggcgata
     9661 gacatcagca cgatcagcgc cgggctaagc tcatgattag gtttagtcat tatttttgtt
     9721 catcaggtga tgattaagct ggaaatcaat aatagcggat gttgatgaat atgggatgaa
     9781 agatagcttg ttgaaatgtg cgatgtggtt tgtaggcatg ataagacgcg ccagcgtcgc
     9841 atcaggcatt gtgcacagaa cggcggatgc ggcgaaaacg ccttatccgc catacggaat
     9901 gagtgtattt tgtaggcatg ataagacgcg tcagcgtcgc atcaggcatt gtgtacagag
     9961 cggcggatga ggcaaaaacg ccttatccgc ctgaataaaa attacttctg cgctgccagc
    10021 gcctcattca cccagccatc gaactgctgc tggtgggctt tgatccagcc atcaacgtgg
    10081 ccctgaatat cgccttctga ggctttgccg tcatgcataa tggcgttctg ggcgttaata
    10141 tctgccactg gcaactgcat aatggcaaac agtttcgctg ctgccgggtt tttctcggcc
    10201 caggctttgt tggcaacgat atgcatggtg ctgaccggga agccataatt cgcaccattc
    10261 ggcagtttgg tatcggcgtt tttatcgccc ggcagtgcgg agaacggcac ctgcaaccag
    10321 acgacatctt tgcccggctt cagttcgtta ctcacccagt acggcgtcca ggtgtagtaa
    10381 aacaccggtt tgccctcttt gtagcgactg atggtgtcgg ccatcatcgc cgcgtagttc
    10441 ccctgattat gcgtcacggt atgggtcagt tcatacgcgg caagctggtg gttgatcgca
    10501 ccttcgcagc cccagccagg gttacaaccg gttaaatccg cttttccgtc gccgttggta
    10561 tcgaacagtt tggcgatctt cggatctttc agttgtgcga tgttggtgat tttgtactgg
    10621 tcggcggttt tcttatcgat caggtaaccc tgtgccgcgc cgttaacaaa taccccttca
    10681 cgataaaatt tcttatcgcc accggcagct tcgtacatgt tgtcatgcag tggcgtccag
    10741 ttcacggcgg tgaaggttgc atcgccggaa gcaagcgagg tgtagccaac gttgtaatcc
    10801 acttcgctgg gtttgttgac ggtataacct aatttctcca gcgcacgact gaccagcagc
    10861 gtctggaagg tttcttcggt gatggtgctt tgaactggat taacagtaat acctttgccc
    10921 ggcagatcgg cagcaaaagt ttgtgtagag ataagcgtgg caaacgctgt cgcaaaaagt
    10981 acgctatgtc gcattgttat tccttttttt ggcagggtga taatgcccgg cgtcaccggg
    11041 caagtgcaga gttacttaat gaatgggcgg gtcagcagac caacagggcc agtggtgtac
    11101 cagcgacggt tgccgcgact gcgtgagtcg cgcccaacgg cctgcgtcag gcgatcgagg
    11161 ataatggcga ggatcacaat cccgacgccg ccaacggtgg caagccccat atccagacga
    11221 ccgataccgc gaagtaccat ctgacccagc ccgccgacgg caatcatcga ggcaatgacc
    11281 accatagaaa gggccagcat cagcgtctgg ttaacgcccg ccataatggt cggcatcgcc
    11341 agcggtaact gaactttgaa cagcatctgg cgcgggctgg caccgaatga gcgcgaggct
    11401 tcaatcagat ccgccggaac ctggttaatc cccagaatgg tcagacggat aatcggcggc
    11461 agagcaaaga tgatcgtcac caccacgccc ggcacgttac cgataccaaa tagcatgacg
    11521 attggcacca gataaacaaa cgctggtgtg gtctgcatgg catcaagcag tggacgaata
    11581 attttcgccg ctcgcggact tctcgccagc cagatcccca acggcaaacc gatgacgata
    11641 cagaacagca gggcggttaa caccagcgcc agagtaacca ttgcctgcga ccaggcaccg
    11701 attgcgccga tggcaatcag cgaaaccagc gtcgccacgc ccattccgac cccggaaatc
    11761 tgccaggcga tgagagcgaa aacgataatc gccaccggtg cgggcatacc cagcagcaac
    11821 tgctggaaac cgttgaggat ataatcaacc ggaacgcgca cgccctggaa gacgggacgg
    11881 aaatgggtaa caacccagtc gatcccttca gtgacccaac tgtcgagcgg gatcagcgtt
    11941 ttatggaacg gatcgagaat attaaaatgc tcgacgtttg gcgcaggcgt actggtcagc
    12001 cagtcagcac caccgccgtc agtcggtgca gtcgccggtg taccccaggc gtctgcggat
    12061 tgcgcggcac tgtccgccgc tggcgtggta tcccacggat tattttgatc agccattatt
    12121 taccccctca cgatctaaag cgcgcagcag cattcctttc gaaatgatgc cgacatactg
    12181 ttggtcctcg tcgaccacgg gcaccgcaca gggtgcctgt ccgacatgag agagcaactc
    12241 gctaagaggc gtttgtgcat cgactgctaa cggcgcatca atcagcgccg catcaagacc
    12301 ttgctgctgc gttaacgcgg ttttaagcga atcgatggag actgcgccga caaacttatt
    12361 accgcgttcg ataacgtagc catattcgcg atcttcatcc tgcaataatt tcagtgccga
    12421 acgtgggccg aagccagggg ttttacgaat taagccattc ggtgtccggc gggcaatatc
    12481 tttcgcactg aatacctgac taatatcaac gccacggaag aaggtacgga cataatcatt
    12541 cgccggatta ttgagaattt catccggtgt gccgacctgt accacttcac cattttgcat
    12601 aatggcaatt cggtcgccaa tacgcatagc ttcatcgaga tcgtgggaaa taaagacaat
    12661 ggtgcgctga tgtttcgcct gtaattttac cagctcatcc tgcatctcgg tgcgaattaa
    12721 tggatcgagc gccgagaagg cttcgtccat taataatata tccggattaa tcgctaacgc
    12781 gcgggctaat cccacacgtt gacgcatccc gccagagagt tcatccgggt agctgtgggc
    12841 ataattttcc agcccgacct gacgcagtgc atcaagggct ttttcccggc gttcttcggc
    12901 attaattccg gccaattcca taccgaacgc agtattgtcc agcacggtca tatgcggcat
    12961 taaggcaaag gactggaaga ccatcgcaat cttttttctg cgcacctcac ggagttcggc
    13021 atcggatatt ttggcaatat ccacaccatc aatcagcact tgcccgcggg tgggttcaat
    13081 caggcgattg agaaggcgta ccattgtgga tttacccgag ccggataatc ccatgatgac
    13141 aaatatctcg ccttcttcaa tggccagact ggcgtctttt acgccaagcg atagcccagt
    13201 tttttccaga atttgttctt tcgaaagtcc ttgttcgata tatttgaacg ctcg
//
LOCUS       DBANUU010000122        13170 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000122 DBANUU010000000
VERSION     DBANUU010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.123"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(543..1634)
                     /gene="ychF"
                     /locus_tag="ACIE68_003607"
     CDS             complement(543..1634)
                     /gene="ychF"
                     /locus_tag="ACIE68_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFX5909864.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVLPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1751..2335)
                     /gene="pth"
                     /locus_tag="ACIE68_003608"
     CDS             complement(1751..2335)
                     /gene="pth"
                     /locus_tag="ACIE68_003608"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFX5909865.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEGVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2613..2891
                     /gene="ychH"
                     /locus_tag="ACIE68_003609"
     CDS             2613..2891
                     /gene="ychH"
                     /locus_tag="ACIE68_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFX5909866.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2946..4625)
                     /gene="dauA"
                     /locus_tag="ACIE68_003610"
     CDS             complement(2946..4625)
                     /gene="dauA"
                     /locus_tag="ACIE68_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFX5909867.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLLTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4750..5697)
                     /gene="prs"
                     /locus_tag="ACIE68_003611"
     CDS             complement(4750..5697)
                     /gene="prs"
                     /locus_tag="ACIE68_003611"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFX5909868.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5848..6699)
                     /gene="ispE"
                     /locus_tag="ACIE68_003612"
     CDS             complement(5848..6699)
                     /gene="ispE"
                     /locus_tag="ACIE68_003612"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFX5909869.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGADISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6699..7322)
                     /gene="lolB"
                     /locus_tag="ACIE68_003613"
     CDS             complement(6699..7322)
                     /gene="lolB"
                     /locus_tag="ACIE68_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFX5909870.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLNEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7536..8792
                     /gene="hemA"
                     /locus_tag="ACIE68_003614"
     CDS             7536..8792
                     /gene="hemA"
                     /locus_tag="ACIE68_003614"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFX5909871.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRGQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8834..9916
                     /gene="prfA"
                     /locus_tag="ACIE68_003615"
     CDS             8834..9916
                     /gene="prfA"
                     /locus_tag="ACIE68_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFX5909872.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9916..10749
                     /gene="prmC"
                     /locus_tag="ACIE68_003616"
     CDS             9916..10749
                     /gene="prmC"
                     /locus_tag="ACIE68_003616"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFX5909873.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGKGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALARERPDCEITAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDLRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10746..11138
                     /gene="sirB2"
                     /locus_tag="ACIE68_003617"
     CDS             10746..11138
                     /gene="sirB2"
                     /locus_tag="ACIE68_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFX5909874.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTMLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11142..11951
                     /gene="sirB1"
                     /locus_tag="ACIE68_003618"
     CDS             11142..11951
                     /gene="sirB1"
                     /locus_tag="ACIE68_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFX5909875.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11987..12841
                     /gene="kdsA"
                     /locus_tag="ACIE68_003619"
     CDS             11987..12841
                     /gene="kdsA"
                     /locus_tag="ACIE68_003619"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFX5909876.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12989..13096)
                     /gene="ldrA"
                     /locus_tag="ACIE68_003620"
     CDS             complement(12989..13096)
                     /gene="ldrA"
                     /locus_tag="ACIE68_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFX5909877.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3291 a         3318 c         3395 g         3166 t
ORIGIN      
        1 tatttttggt aataatccta attagttatg ggattttagc ttgatgtatc tgcataacaa
       61 aaacaatatg cgccattttt gcattactaa taagaaaaag caaaaaacat cgatattctt
      121 ttttgaacaa tggattttct ggcatatcgt tattatcgtg ctattggtta tcattttcat
      181 cttgccagga tcgcacgtaa tcacgttatt cggggacttt atctgcggga tattaccctt
      241 caggtaagtc cttttctcgc cagcatattg gagcgtgatc aatattgatc agctgtgaag
      301 agggctgatg tgtagtggaa caaaaactga cgttatatca ctgctaatga gatatccgtc
      361 atctgaacac ttatgtcttt aattattctg gtggttcact gtaggcaata agcacaaaag
      421 tgtaggatgt tacaagaatg attaggactc ggtgaaatga aaaatccacg caattgcgtg
      481 gattttatat acttttgcgc tcttcatgag atttagcgaa acctcatgag acaacaaatt
      541 aattagacgt tgaaaaggaa gttcatcaca tcgccatctt taacgatgta atctttacct
      601 tctgcacgca ttttgccagc ttctttcgcg ccttgttcac ctttgtaagt gatgaaatct
      661 tcaaacgaga tggtttgtgc acggataaag cctttttcaa aatcagtatg gattttgccc
      721 gctgcctgcg gcgcggttgc tccaaccgga atggtccatg cacgcacttc tttcacccca
      781 gcggtgaagt aagtttgcag gttcagcagc ttataaccgg cacggatcac acggttcagg
      841 cccggctctt ccagcccaag ctcctgcata aactcgtcac gttcttcgtc gtccagttcg
      901 gcaatgtctg cttcaacagc agcacaaacc ggaagcacaa cagaaccttc tttcgccgcg
      961 atttcacgca cctggtcaag atatgggttg ttttcaaaac cgtcttcgtt gacgttggcg
     1021 atgtacattg ttggtttcag cgtcaggaag ctcaggtaac gaatagccgc tttctcttca
     1081 gcgcttaaat ccagcgcgcg cagcatacct gcgttttcca gctggggcag gcatttttcc
     1141 aggactgcca gctcggcttt cgcgtcttta tcgccacctt tggctttctt ctgtacgcga
     1201 tgaatcgcac gttcgcaggt gtcgaggtct gccagcgcca gttcggtgtt aataacttca
     1261 atatcgtcag ccgggttaac tttgccggaa acgtgaatga tgttgtcatt ttcaaagcag
     1321 cgaacaacgt gaccgatcgc ttcggtttca cggatgttgg tcaggaactg gttacccaga
     1381 ccttcgcctt tcgatgcgcc ttttaccaga ccggcgatat cgacaaattc catggtcgtg
     1441 ggaagcgtac gttgcggttt tacgatttca gccagttgat ccaggcgagg atcaggcatt
     1501 ggtacgacgc ctgtgttcgg ctcaatggtg cagaatggaa agttggccgc ttcaataccg
     1561 gctttggtca gcgcgttgaa cagggtagat ttcccgacgt tgggcaaacc gacgataccg
     1621 catttgaatc ccatgattta aatcacctta atatcttaat aatcaacctg ttattgctaa
     1681 cagattgcag aaatggaaat aactttgcct attatacacg gcactcggca aaaatgccgc
     1741 agacaacgac ttattgcgct ttaaaggcgt gcaatcggtt cgttgctttg gtcaagccat
     1801 ctgtaaacca catttcagta caacgcgccg cttcgtcgat ggcttcatca attaacttct
     1861 gttcactaac aggcggtttg cctaacacaa aaccgacaac tttattttta tcgcccggat
     1921 gaccgattcc gatgcgtaaa cggtgaaagt tagggttatt acccaattta ctgatgatgt
     1981 ctttcagtcc attgtgacca ccatggccac cgcccaactt aaatttggcg acgccaggag
     2041 gcagatccag ttcgtcgtgg gccaccagaa tttcgtccgg attaatgcgg aaaaaactgg
     2101 ccatcgccgc aacggctttg ccgctgagat tcataaatgt agtcgggact aacaggcgga
     2161 caccttcgcc tccaagggtg actcgcgaag tataaccaaa gaatttagcc tcttcgcgca
     2221 gcggagcgcg caaacgctct gccagtaagt caacgaacca ggcaccagca ttatgtcgcg
     2281 ttgcggcgta ttcagcaccg gggttcgcca ggccgacaat caatttaatc gtcacgtttt
     2341 tttgtcctga gtgtgtacat aactggcgcg tagtttactg gttgcggccc cgcttgacaa
     2401 aaaactgcgt atcaaatgca gataacgtaa taattgcctg agtggactat tagaaagtca
     2461 gggtgttcag gcgtttattt gtaaagtttt gttgaaataa gggttgtaat tgtgatcacg
     2521 cccgcacata acccacaggg tgttgtctat actttacaca taaggaagag gggtattccc
     2581 tgttacaacc cagaaagttc cggaggtgac atatgaaacg caaaaacgct tcgttactcg
     2641 gtaacgtgct catggggttg ggtctggtgg taatggtggt cggtgtgggg tattcaatcc
     2701 tcaaccagtt accacagttt aatatgcccc agtatttcgc acatggtgca gtgctaagta
     2761 ttttcgtcgg tgccattctc tggctggcgg gtgcccgtgt tggcgggcat gaacaggtgt
     2821 gcgaccgtta ctggtgggtt cgccactatg acaaacgttg ccgccgtagc gataatcgcc
     2881 gtcatagcta acaaataatg cagtttgctg accagtcagt ttgcgctgac tggtcaatct
     2941 cgtacttata aatccgccat cgccgcgcga cgattcggga agaacgccag gcgtcccggg
     3001 atcggttgaa tgccagcgcg agccatagtg cgcagtggct ggaattccac gttgcacacg
     3061 cgcagttcac atccctcggg cagacgcttc acaaaacgct ggaacgcatc aagaccacca
     3121 gcatcaagta ccggaacggc atcccacttc agaatcacaa tccgtttgcc ttcaagacgt
     3181 gactccaggt ccgtgagcaa gccttcagca gcagcaaaaa acagcgggcc aataacacgc
     3241 agaaccagga catcgtctgg aacatctacg actaccggtg ccaggcgagt catacgtgcg
     3301 atacgacgca taaacagcag cgatgccagc acgatcccca cgctgatggc aataaccata
     3361 tcaaacagca cggtcaacga catgcacagc agcatgacaa tgatgtcatc tttcggcgca
     3421 tgacgcagca agtcgaccac tttgtgcgct tcactcatgt tccacgccac catcaacagc
     3481 agggctgcca tggcggaaag cggcagccag gagagcagcg gtgccagtac cagcagggca
     3541 agaataacca gaatagagtg gatcaccgcc gagatagggg aagttgcccc ggcacggacg
     3601 ttagcggcag aacgcgcgat ggcagctgta gcggtaatac caccaaagaa cggagcgata
     3661 atattcccca gtccctgtcc aaccagttcg ctgtttgcct tgtgttttgt cccggtcata
     3721 ccatccagca ccacggcgca gagcagagat tcgattgcgc cgagcattgc cattgagaat
     3781 gccgcaggca gcagtgtgcg aatagaatcc caggtaagcg tgaattctga attaggcaga
     3841 tcccacggca gcaccagttg cggcagcagt tgcggaatac cgttaccctg agaaccatcg
     3901 gccaggacgt agtggaattg cgaaccgatg gtagcaacat gtccgccgag caggttaaca
     3961 atccccatca ccgcgcaacc agccagcaag gccggaaggt gaccgggtaa acgaatgccc
     4021 agacgcggcc agaaaaccag aatgccaagc gtcacaatgc caatggcagc atcacccaca
     4081 ttaatggtcg gcagcgccat aaataatgcg ccgacttttt gtagataatg ttccgggaca
     4141 tgggccattt gcagaccgag aaaatcttta atctgcatgg taccgatggt gatcccgata
     4201 cccgaggtga aacctaaggt gacggaaacc ggaatatact caatcaggcg accaaagcgt
     4261 gccagaccca tcagaatcaa aaagatcccc gacagcaagg tcgcaaccag cagtcctgcc
     4321 agtccaaact gttgcgacac gggatagaga attaccacaa atgccgcagt cggaccggaa
     4381 acgctaaagc gtgatccacc cgtcagagca atgacaatcc ccgcaacagc tgcagtatat
     4441 aaaccgtact ggggtgccac accactacca atagccaacg ccatcgccag cgggatagca
     4501 ataatcccga cggttatccc ggcaatcagg tcacgggtaa accgtgcggc agtatatttt
     4561 tctttccagc aagcgtcgat cagagcgcgg aaaggcatca catgtgagga aaatattttg
     4621 ttcacaataa tgtttcatcc gtgagcgcat catctgtcaa ctaaatggca ggtgaaggag
     4681 gcataggtca tacaaatgga tattacagac aaaaaaaccc gccgcagcgg gtctttgagc
     4741 cgggttcgat tagtgttcga acatggcaga gatcgattct tcgttgctga tacgacgaat
     4801 cgcttcggcc agcatacctg acagggtcag agtacgcacg ttcggcagtg atttgatttc
     4861 atcgctcagc ggaatggtat cgcagacaac gacttcatca attacagagt tacgcaggtt
     4921 gttcgccgcg ttgccagaga agatcgggtg agtcgcgtac gcaaatacac gtttagcacc
     4981 acgttctttc agagcttcag cagctttaca cagcgtaccg ccagtgtcga tcatatcatc
     5041 gaccagtacg cagtcacgac ctgcaacgtc accgatgata tgcatcacct gggaaacgtt
     5101 cgcacgcgga cgacgtttgt cgatgattgc catatcggta tcgttcagca gcttagcgat
     5161 agcgcgggca cgcacaacgc cgccgatgtc cggagaaacc acaattgggt tatccagatt
     5221 cagctgcagc atgtcttcca gcaggatcgg gctaccaaat acgttatcaa ccggaacgtc
     5281 gaagaaaccc tgaatctgtt cagcgtgcag atccactgtc agcacacggt caacaccgac
     5341 gctggagagg aagtctgcaa ccactttcgc agtgattggt acacgagcgg aacggacgcg
     5401 acggtcctgg cgcgcatagc caaagtaggg gataacagcg gtgatacgac ctgcggaagc
     5461 acgacgcagg gcatcaacca taacgactaa ttccatcagg ttgtcgttag taggggcaca
     5521 agtggactgg atgatgaaaa tatcaccacc gcgtacattt tcattaattt gtacgctgac
     5581 ttcgccgtcg ctaaagcgac ctacagcggc gtcgccgagt gaagtgtaca ggcggttggc
     5641 aatacgttgt gctagttccg gggtggcgtt accagcaaaa agcttcatat caggcacgag
     5701 aagaacctca ggcatgcgtc cattggtgga aagaatctgc cgaaaactgt gcgggccagg
     5761 catgatcctt tccaggcggt gtatttaaga gcgcgatgca acgtctggaa caaggtgacg
     5821 ttgtcaccga aactcagctt gcccggctta aagcatggct ctgtgcaatg gggaaagatt
     5881 aacgcctttc gccacaaagc cattgagcca ttccggggct tgctctagca cctggcgggc
     5941 ttcagactct gtatcaaatt cagcaaagac acaggcccct gtcccagtca ggcgcgacgg
     6001 ggcgtattct aacagccagg aaagcaccgc atcaacctcg cgaaaacgtt ttcttgcgat
     6061 aacctcgcaa tcattgctga attcacattt tagcaacgtt tctattgacc tttttggcgt
     6121 attgcgcggg agttcaggat ctttaaaaat caccggagtc ggaatactta caccagggtg
     6181 cgccaccaga taccacttct ctggcggatc caccggcgtt agtatttcac caacgccttc
     6241 ggcaaacgcg gcatgccccc gaacaaagac aggaacatct gcgcccagcg tcagccccat
     6301 ttccgccagc tcatccatgc ttagcccgca ttgccagaga tgatttaatg ccaccaggac
     6361 cgtcgcggca ttggatgaac caccgccgag accgccgccc attggcaaac gcttgtcaat
     6421 gctgatatcc gcaccgcttc ccgtcggaag acgcccgctg tctgccgcag ttttcatcaa
     6481 caaccgcgct gcgcgaacga tcaggttatc ttcatgttcc acgccttcaa cgggcgttaa
     6541 cagacgaata tccccatcgt cacgaagctc aatgctaatg gtgtcgccgt aatcaagaaa
     6601 ctgaaacagc gtttgcagcg tgtggtaacc atccgcacgc tgaccggtaa tgtataaaaa
     6661 cagattaagt tttgccggag agggccactg tgtccgcatt atttcactat ccagttatcc
     6721 atttttaact tgatgcgttg accaccgtcg gtgagttcca tattggctgg catcgcaggt
     6781 tgcgttttgg tgtcataacc accgtaaaca actttccagt ttttgccatt ctggctgtag
     6841 gtaatttcgt tcaggcggta ctggtcgtcc agtttgtagt cggttgcatc acccggtaaa
     6901 cctaaaatcc actggcgcaa gctgttgagc ggaatcggca ttccggtcaa tttgccaatc
     6961 atctcttcgg cgtcatcggc ggtataacgc tgacctttat tgtcgactaa ctgcacgtta
     7021 cccggttgag cattcagctc aagttccgta ctgcccaatg ggttagtcag cagcagacgg
     7081 tagcgatcct gaccagtttg ctgccagaag aaacgggcgt acactttttg ttggtcagaa
     7141 atataagcga acgcgccgcg agtttgatac tgattaagat tgcgcacgtc ttgctgatgc
     7201 tgacgccatt gtggcgaatc cgggctttta ccaggacctt tgggcgtggt aacggaacag
     7261 gcagtgagca caagagcagc cagcggtagc aggcggataa gacgaaaatc gggcaggggc
     7321 atagtgatga caagtccttg agatacgttg cagttataac ccttaatgct agcgttacca
     7381 tccgctatcg tctatgttca agttgtctta attgccagaa tctaacggct ttcggcaatt
     7441 actccaaaag ggggcgctct cttttattga tcttacgcat cctgtatgat gcaagcagac
     7501 taaccctatc aacgttggta ttatttcccg cagacatgac ccttttagca ctcggtatca
     7561 accataaaac ggcacctgta tcgctgcgag aacgtgtatc gttttcgccg gataagctcg
     7621 atcaggcgct tgacagcctg cttgcgcagc cgatggtgca gggcggcgtg gtgctgtcga
     7681 cgtgcaatcg cacggaactt tatcttagcg ttgaagagca ggataacctg caagaggcgt
     7741 taatccgctg gctttgcgat tatcacaatc ttaatgaaga agatctgcgt aaaagcctct
     7801 actggcatca ggataacgac gcggttagcc atttaatgcg tgttgccagc ggcctggatt
     7861 cactggttct gggggagccg cagatccttg gtcaggttaa aaaagcgttt gccgattcgc
     7921 aaaaaggcca tatgaaggcc agcgaactgg aacgcatgtt ccagaaatct ttctctgtag
     7981 cgaaacgcgt tcgcactgaa acagatatcg gtgccagcgc tgtgtctgtc gcttttgcgg
     8041 cttgtacgct ggcgcggcag atctttgaat cgctctctac ggtcacagtg ttgctggtag
     8101 gcgcgggcga aaccatcgag ctggtagcgc gtcatctgcg cgaacataaa gtacagaaga
     8161 tgattatcgc caaccgcact cgcgaacgtg cccaaattct ggcagatgaa gtcggcgcgg
     8221 aagtgattgc cctgagcgat atcgacgaac gtctgcgcga agccgatatc atcatcagtt
     8281 ccaccgccag cccgttaccg attatcggga aaggcatggt ggagcgcgca ttaaaaagcc
     8341 gtcgcaacca accaatgctg ttggtggata ttgccgttcc gcgcgatgtt gagccggaag
     8401 ttggtaaact ggcgaatgct tatctttata gcgttgacga tctgcaaagc atcatttcgc
     8461 acaacctggc gcagcgtaaa gccgcagcgg ttgaggcgga aactattgtc gctcaggaaa
     8521 ccagcgaatt tatggcgtgg ctgcgagcac aaagcgccag cgaaaccatt cgcgagtatc
     8581 gcggccaggc agagcaagtt cgcgatgagt taaccgccaa agcgttagcg gcccttgagc
     8641 agggcggcga cgcgcaagcc attatgcagg atctggcatg gaaactgact aaccgcttga
     8701 tccatgcgcc aacgaaatca cttcaacagg ccgcccgtga cggggataac gaacgcctga
     8761 atattctgcg cgacagcctc gggctggagt agcagtacat cattttcttt ttttacaggg
     8821 tgcatttacg cctatgaagc cttctatcgt tgccaaactg gaagccctgc atgaacgcca
     8881 tgaagaagtt caggcgttgc tgggtgacgc gcaaactatc gccgaccagg aacgttttcg
     8941 cgcattatca cgcgaatatg cgcagttaag tgatgtttcg cgctgtttta ccgactggca
     9001 acaggttcag gaagatatcg aaaccgcaca gatgatgctc gacgatcctg aaatgcgtga
     9061 gatggcgcag gatgaactgc gcgaagctaa agaaaaaagc gagcaactgg aacagcaatt
     9121 acaggttctg ttactaccaa aagatcctga tgacgaacgt aacgccttcc tcgaagtccg
     9181 tgccggaacc ggtggcgacg aagcggcgct gttcgcaggc gatctgttcc gtatgtacag
     9241 ccgttatgcc gaagcccgcc gctggcgggt agaaatcatg agcgccagcg agggtgaaca
     9301 tggtggttat aaagagatca tcgccaaaat tagcggtgat ggtgtgtatg gtcgtctgaa
     9361 atttgaatcc ggcggtcatc gcgtgcaacg tgttcctgct acggaatcgc agggtcgtat
     9421 tcatacttct gcttgtaccg ttgcggtaat gccagaactg cctgacgcag aactgccgga
     9481 catcaaccca gcagatttac gcattgatac tttccgctcg tcaggggcgg gtggtcagca
     9541 cgttaacacc accgattcgg caattcgtat tactcacttg ccgaccggga ttgttgttga
     9601 atgtcaggac gaacgttcac aacataaaaa caaagctaaa gcactttctg ttctcggtgc
     9661 tcgcatccac gctgctgaaa tggcaaaacg ccaacaggcc gaagcgtcta cccgtcgtaa
     9721 cctgctgggg agtggcgatc gcagcgaccg taaccgtact tacaacttcc cgcaggggcg
     9781 cgttaccgat caccgcatca acctgacgct ctaccgcctg gatgaagtga tggaaggtaa
     9841 gctggatatg ctgattgaac cgattatcca ggaacatcag gccgaccaac tggcggcgtt
     9901 gtccgagcag gaataatgga atatcaacac tggttacgtg aagcaataag ccaacttcag
     9961 gcgagcgaaa gcccgcggcg tgatgctgaa atcctgctgg aacatgttac cggcaaaggg
    10021 cgtactttta tcctcgcctt tggtgaaacg cagctgactg acgaacaatg tcagcaactt
    10081 gatgcgctac tgacacgtcg tcgcgatggt gaacccattg ctcatttaac cggggtgcga
    10141 gaattctggt cgttgccgtt atttgtttcg ccagcgacct taattccgcg cccggatacg
    10201 gagtgtctgg tggagcaggc actggcgcgg ttgcctgaac aaccttgccg tattctcgat
    10261 ctcgggacgg gtaccggggc gattgcgctt gcgctggctc gcgagcgccc ggactgcgaa
    10321 attaccgctg tagatcgtat gcccgatgct gtcgccctgg cacaacgtaa tgcccagcat
    10381 ctggcgatca aaaatatcca cattctgcaa agcgactggt ttagcgcgct agccgggcag
    10441 cagtttgcga tgattgtcag caatccgccg tatattgacg agcaggaccc tcatcttcaa
    10501 caaggcgatc tccgctttga gccgctcact gcgctggttg cggcagacag tggaatggca
    10561 gacatcgtgc atatcatcga acagtcgcgt aacgcgctgg tatccggcgg ctttctgctt
    10621 ctggaacatg gctggcagca gggcgaagcg gtgcggcagg catttatcct cgcggggtat
    10681 catgacgtcg aaacctgccg tgactatggt gataacgagc gcgtaacgct cggccgctat
    10741 tatcaatgac aagtttttct acactgctta gtgttcatct tattagtatc gcgctttctg
    10801 ttgggctatt aaccttacgt ttctggctac gttatcagaa gcatccacag gcatttgctc
    10861 gctggacgcg cattgtgccg ccggttgtcg atacgatgtt attgttaagc ggcattgcgt
    10921 tgatggctaa agcgcacatc ctgccatttt ctgggcaggc acagtggctg actgaaaagc
    10981 tgtttggagt tatcatttat atcgttttgg gttttattgc actcgattat cgtcgtatgc
    11041 acagtcagca ggcgcgcatt attgccttcc cgctggcgtt ggtggtgctg tacatcatca
    11101 ttaaactcgc caccacaaaa gtaccgttac tggggtaagt catgagatcg ttagctgatt
    11161 tcgaatttaa taaagcgcca ttgtgcgaag gcatgatcct ggcttgcgaa gcaatccgcc
    11221 gcgattttcc ctcgcaagat gtttacgacg aactggagcg tctcgttagt ctggcgaagg
    11281 aagaaatcag ccagcttctg cctttagaag agcagctgga aaaactgatc gcgctgtttt
    11341 acggcgactg gggatttaaa gcctcacgcg gcgtttatcg tctttccgat gcattatggc
    11401 tggaccaggt gttaaagaat cgacagggca gtgcggtatc attaggtgcg gttttattat
    11461 gggtcgcgaa tcgtctcgat ttgccgctgc tgccggtgat tttccctacg cagctgatat
    11521 tgcgcattga atgtccggat ggcgaaattt ggctgattaa tccttttaac ggtgaatcgt
    11581 taagcgaaca tatgctggac gtatggttaa agggaaatat cagcccgtcg gcggaactgt
    11641 tttatgaaga ccttgatgaa gctgataaca ttgaggtaat ccgcaaattg ctggatacac
    11701 tcaaagcctc gttgatggaa gaaaatcaga tggagctggc gttacgcacc agcgaagctt
    11761 tattacaatt caaccctgaa gatccctatg aaattcgcga tcgcgggttg atttatgcgc
    11821 aactggattg cgaacacgtt gcgttgaacg atttaagtta tttcgttgaa cagtgtccgg
    11881 aagacccgat cagcgaaatg atccgtgcgc aaataaataa catcgcgcat aaacatattg
    11941 tgctgcatta attaatcgac attttactca agattaaggc gatcctatga aacaaaaagt
    12001 ggttagcatt ggcgacatca acgtagcaaa tgacctgccg ttcgtactgt ttggtggtat
    12061 gaacgtgctg gaatctcgcg atctggcgat gcgcatttgc gagcactacg taaccgtgac
    12121 ccagaaactg ggtatccctt acgtgttcaa agcctctttt gacaaagcca accgctcctc
    12181 catccactct tatcgtggac cgggcctgga agaagggatg aaaatcttcc aggaattgaa
    12241 gcagactttt ggcgtgaaaa ttatcaccga cgttcacgaa ccaagtcagg cacagcccgt
    12301 tgctgatgtc gtggatgtga ttcagttgcc ggcgtttctt gctcgccaga ctgacctggt
    12361 tgaagccatg gcgaaaaccg gtgcggtaat taacgtcaag aaaccacagt ttgtcagccc
    12421 gggacagatg ggtaatatcg ttgataaatt caaagaaggc ggcaacgaaa aagtgattct
    12481 ttgcgatcgc ggtgctaact tcggctatga caacctggtt gtcgatatgc tgggcttcag
    12541 cattatgaag aaagtgtctg gtaactcgcc ggtgattttc gacgtgaccc acgcactgca
    12601 atgccgcgat ccttttggcg cagcttccgg cggtcgtcgt gctcaggtgg ctgagctggc
    12661 acgagccggt atggcggtag gtctggcggg gctgtttatt gaagcgcatc cggatccgga
    12721 acatgcgaaa tgtgatggtc catccgcgct gccgctggct aaactggaac cgttcctcaa
    12781 gcagatgaaa gcgattgatg atctggtgaa aggtttcgaa gaactggata ccagcaagta
    12841 atctttttgt ttgaaaaata aagtcttagc gttctgcgtt aagacttttt ttatgggtgc
    12901 cggatacaaa aaaggccgca ggctgttacc cctgcggccg gtttcgggcg catattgcca
    12961 tcacggcagc ctgacgcccg ttttcgcctt acttccggtt acgccaccag ccgacaatcg
    13021 ctgcggtaat aattcccgcc aggatcggtg ctgccaggtc gtgccagaaa atcatggcaa
    13081 actgcgcgag cgtcatatag ccgccttgtt gtaatgacaa cattttgcgg ctattcttga
    13141 attgttgatg gttcaagatt agcccccgtt
//
LOCUS       DBANUU010000123        12774 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000123 DBANUU010000000
VERSION     DBANUU010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.124"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            237..1751
                     /gene="caiT"
                     /locus_tag="ACIE68_003621"
     CDS             237..1751
                     /gene="caiT"
                     /locus_tag="ACIE68_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFX5909878.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1782..2924
                     /gene="caiA"
                     /locus_tag="ACIE68_003622"
     CDS             1782..2924
                     /gene="caiA"
                     /locus_tag="ACIE68_003622"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFX5909879.1"
                     /translation="MEFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLLPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDAVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            3042..4259
                     /gene="caiB"
                     /locus_tag="ACIE68_003623"
     CDS             3042..4259
                     /gene="caiB"
                     /locus_tag="ACIE68_003623"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFX5909880.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGKIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4333..5886
                     /gene="caiC"
                     /locus_tag="ACIE68_003624"
     CDS             4333..5886
                     /gene="caiC"
                     /locus_tag="ACIE68_003624"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFX5909881.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINAHLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5995..6780
                     /gene="caiD"
                     /locus_tag="ACIE68_003625"
     CDS             5995..6780
                     /gene="caiD"
                     /locus_tag="ACIE68_003625"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFX5909882.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6786..7376
                     /gene="caiE"
                     /locus_tag="ACIE68_003626"
     CDS             6786..7376
                     /gene="caiE"
                     /locus_tag="ACIE68_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFX5909883.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGADVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDNDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRTVSDGELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7495..7890)
                     /gene="caiF"
                     /locus_tag="ACIE68_003627"
     CDS             complement(7495..7890)
                     /gene="caiF"
                     /locus_tag="ACIE68_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFX5909884.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8151..11372)
                     /gene="carB"
                     /locus_tag="ACIE68_003628"
     CDS             complement(8151..11372)
                     /gene="carB"
                     /locus_tag="ACIE68_003628"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFX5909885.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11390..12538)
                     /gene="carA"
                     /locus_tag="ACIE68_003629"
     CDS             complement(11390..12538)
                     /gene="carA"
                     /locus_tag="ACIE68_003629"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFX5909886.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAN"
BASE COUNT         2994 a         3332 c         3367 g         3081 t
ORIGIN      
        1 ttttactcac aatcaaactc atcacattga taaacaatga atatttacac aaagacgccc
       61 tgtttttacc cgataaaaaa ctgtgactaa catcacagaa tgcagccaat tgaataccca
      121 ttatgagtta gcaattaacg cgtccacgag gttaataata attatattaa atgttaacaa
      181 aaataaaaca aacgggagcg caaaataaat gttcgttttc acagtggaat taactcatga
      241 agaatgaaaa gagaaaaacg ggaatagaac cgaaggtttt ctttccgccg ttaataatcg
      301 tcggcatact ttgttggctt acagtcagag atctggatgc agcgaatgtc gttattaatg
      361 ctgtattcag ttacgtcacc aatgtatggg gatgggcatt tgaatggtat atggtagtga
      421 tgcttttcgg ttggttctgg ctggtgtttg gcccgtatgc caaaaagcgt ttaggtaacg
      481 aaccacctga atttagtacc gccagttgga tctttatgat gttcgcctcc tgtacgtctg
      541 ctgccgtact gttctgggga tcgattgaga tctactacta catctccacc ccgccgtttg
      601 gcttagaacc gaactcgaca ggggcgaaag agttggggct ggcttacagc ttgttccact
      661 ggggacctct gccgtgggcc acttacagct tcctttcagt cgccttcgct tacttcttct
      721 ttgtccgcaa aatggaagtg attcgcccca gctcgacact agtgccgctg gtaggtgaaa
      781 aacacgccaa agggttgttc ggcactatcg tcgacaactt ctatctcgtc gccttgatct
      841 tcgcgatggg caccagtctg ggccttgcca cgccgctggt gaccgagtgt atgcaatggt
      901 tgtttggcat tccgcatacc ctgcaactgg acgctatcat cattacctgc tggattatcc
      961 tcaacgccat ttgtgtcgcc tgcggtctgc aaaaaggggt acgtatcgcc agtgacgtgc
     1021 gtagttacct gagcttcctg atgctgggtt gggtgttcat tgtcagcggt gccagcttca
     1081 tcatgaacta cttcaccgat tcggtgggga tgttgctgat gtatctgccg cgcatgttgt
     1141 tctataccga tcccatcgct aaaggcggct tcccgcaggg ctggaccgtg ttctactggg
     1201 catggtgggt gatttacgct atccagatga gtatcttcct cgcccgcatc tcccgtggtc
     1261 gtactgtgcg tgaactgtgc ttcggcatgg tgatggggct gacagcgtca acctggatcc
     1321 tgtggaccgt gctcggtagt aacactctgc tgttgatgga taaaaacatc atcaacattc
     1381 caaatctgat cgaacagtac ggtgtggcgc gcgccatcat cgaaacctgg gccgctctgc
     1441 cactcagcac cgccaccatg tggggcttct tcatcctctg ctttattgcc accgttacgc
     1501 tggttaacgc ctgctcttat accctggcga tgtccacttg ccgcgaagta cgcgatggtg
     1561 aagagccacc gctgctggtg cgtatcggct ggtcaattct ggttggcatt atcggtattg
     1621 ttctgctggc gctcggcggc ctgaaaccga ttcaaaccgc cattatcgcc ggaggatgcc
     1681 cgctgttctt cgtcaacatt atggtgacgc tctcctttat taaagacgcg aaacagaact
     1741 ggaaagatta attaaccccc aaaatatcaa gaggttgaaa gatggaattt aatttaaatg
     1801 atgagcagga actgtttgtc gccggtatcc gcgaactgat ggccagcgaa aactgggagg
     1861 cctattttgc cgagtgcgac cgtgacagcg tctacccgga acgttttgtc aaagcactgg
     1921 cggatatggg tatcgacagt ctgctgctcc ctgaagagca cggtggtctg gacgcggggt
     1981 ttgttactct cgccgccgtg tggatggagc tgggacgtct gggggcacca acctatgtgc
     2041 tgtaccagtt gccgggcggg ttcaacacct tcctgcgcga aggcacacaa gagcagatcg
     2101 acaaaattat ggctttccgc ggcaccggta agcagatgtg gaactcagcg attaccgaac
     2161 cgggcgcggg ctccgacgtg ggtagcctga aaacgactta tacccgtaga aatggtaaga
     2221 tttatcttaa tggtagtaag tgttttatta ccagcagcgc ctacaccccg tacatcgtgg
     2281 tgatggcgcg cgacggggct tctccggaca aacctgtcta caccgaatgg tttgttgata
     2341 tgagcaaacc gggcatcaaa gtgaccaaac ttgaaaagct cggtctgcgt atggatagct
     2401 gctgtgaaat cacctttgac gctgtggaac tggacgagaa agacatgttc ggtcgggaag
     2461 gtaacggctt taaccgcgtc aaagaagagt tcgaccatga acgtttcctg gtagccctga
     2521 ccaactacgg tacggcgatg tgcgcctttg aagatgcggc gcgctacgcc aaccagcgcg
     2581 tgcagtttgg cgaggctatt ggtcgtttcc agttgattca ggaaaaattc gcccacatgg
     2641 cgatcaaatt aaactccatg aaaaacatgc tgtatgaagc agcgtggaaa gcagacaacg
     2701 gcaccatcac ctctggcgat gcagcgatgt gcaaatactt ctgcgccaat gcggcatttg
     2761 aagtggtgga tagcgcaatg caggtgctgg gcggtgtcgg gatcgcgggc aaccaccgca
     2821 tcagccgctt ctggcgtgac ctgcgtgtag accgagtctc tgggggatct gacgaaatgc
     2881 agatcctgac gctgggtcgc gcggtgctga agcaataccg ctaagtccgt gcgctgcctg
     2941 atgcgacgct gacgcgtctt atcaggccta cggtcggccg gataaggcgt ttacgccaca
     3001 tccggcagtc atgcgctaca taaatttttc aggagctaat aatggatcat ctacccatgc
     3061 cgaaattcgg gccgctggcc ggattgcgcg ttgtcttctc cggtatcgaa atcgccgggc
     3121 cgttcgccgg gcaaatgttc gcagaatggg gcgcggaagt tatttggatc gagaacgtcg
     3181 cctgggccga caccattcgc gttcaaccta actacccgca gctctcccgc cgcaatttgc
     3241 acgcgctgtc gttaaatatc ttcaaagatg aaggccgcga agcgtttctg aaattaatgg
     3301 aaaccaccga tatcttcatc gaagccagta aaggtccggc atttgcccgt cgtggcatta
     3361 ccgatgaagt cctgtggcag cacaacccga aactggttat cgctcacctg tccggttttg
     3421 gtcagtacgg caccgaggag tacaccaatc ttccggccta taacaccatc gcccaggcct
     3481 tcagtggtta cctgattcag aacggtgatg ttgaccagcc aatgcctgcc ttcccgtata
     3541 ccgccgatta cttttctggc ctgaccgcca ccacggcggc gctggcagca ctgcataaag
     3601 tgcgtgaaac cggtaaaggc gaaagtatcg acatcgccat gtatgaagtg atgctgcgta
     3661 tgggccagta cttcatgatg gattacttca acggcggcga aatgtgcccg cgcatgagca
     3721 aaggtaaaga tccctactac gccggttgcg gtctgtataa atgcgccgac ggctacatcg
     3781 tgatggaact ggtgggcatt actcaaattg cagagtgctt taaagatatt ggcctcgctc
     3841 atctgcttgg cacgccagaa atcccggaag gcactcagct tatccaccgt atcgaatgcc
     3901 cttacggccc actggtagaa gagaaactcg atgcctggct ggctgcacat accatcgcag
     3961 aagttaaaga acgctttgcc gaactgaata tcgcctgtgc caaagtactg accgtaccgg
     4021 aactggaaag caatccacag tatgtggctc gcgaatcaat cactcagtgg caaacgatgg
     4081 atggtcgcac ctgcaagggg ccgaatatca tgccgaaatt caaaaataac cccggaaaaa
     4141 tctggcgcgg aatgccctca catggcatgg acacggctgc cattttgaaa aatatcggct
     4201 acagcgaaaa cgacattcag gagttggtca gcaaaggtct ggccaaagtt gaggactaaa
     4261 cgcgtggcgt cagtcggctt gcctgactag cgcccgccca ataacaaacg aacaacaatg
     4321 gatagaggtg caatggatat cattggcgga caacatctac gtcaaatgtg ggacgatctg
     4381 gcggacgttt acggtcataa aacggcgctg atttgtgaat ccagcggcgg agtcgttaac
     4441 cggtatagtt atcttgagtt aaatcaggag attaaccgca cggcaaacct gttttatacg
     4501 ctggggattc gcaaaggcga caaggttgca ctacatctcg acaactgccc ggaatttatc
     4561 ttttgctggt tcgggctggc aaaaattggc gcgattatgg tgccgattaa cgcccacctg
     4621 ttacgcgagg aaagcgcgtg gatcctgcaa aatagccagg cgtgcctgct ggtgaccagt
     4681 gcgcaattct atcctatgta tcaacagatt cagcaggaag atgccactca attgcggcac
     4741 atttgcctga cagatgtggc acttcccgct gatgatggcg tgagttcgtt tactcaactg
     4801 aaaaatcaac aacctgccac cttgtgctat gcaccgccgc tatcgactga cgatacggcg
     4861 gaaattctct tcacctccgg caccacctcc cgaccgaaag gtgtggtgat tacccattac
     4921 aacctgcgct tcgctggata ttactccgcc tggcagtgtg cactgcgtga cgatgacgtc
     4981 tacctgacgg taatgcctgc gtttcatatc gattgccagt gtactgcggc gatggcggcg
     5041 ttttctgccg gggccacctt tgtgctggtc gagaaataca gcgcccgcgc cttctgggga
     5101 caggtgcaga agtaccgcgc caccattacc gaatgtattc cgatgatgat tcgtacgttg
     5161 atggtgcagc cgccttcagc gaacgatcgg caacaccgcc tgcgggaagt gatgttttat
     5221 ctcaacttgt cggagcagga aaaagatgcg ttttgtgaac gcttcggcgt tcgcttgctg
     5281 acgtcttatg ggatgacgga aaccattgtg ggcattatcg gcgatcgtcc tggcgataaa
     5341 cgacgctggc cgtcgattgg tcgggcgggg ttttgctacg aagcggagat ccgcgacgat
     5401 cacaatcgcc cgctcccggc aggtgagatc ggtgaaatct gtattaaagg cgtaccaggg
     5461 aaaaccatct tcaaagagta ttttctcaac ccgaaagcca ctgcaaaagt gctggaagct
     5521 gatggctggc tgcataccgg cgataccgga taccgagacg aagagggctt tttttatttc
     5581 gtcgatcgcc gctgcaatat gatcaaacgc ggtggcgaga atgtctcctg cgtggagctc
     5641 gaaaatatca tcgccaccca tccaaaaatt caggatatcg tggttgtggg tattaaagat
     5701 tcgattcgcg atgaagccat caaagcattt gtggtgctga atgaaggtga aacattgagc
     5761 gaagaggaat ttttccgctt ctgcgaacaa aatatggcga aatttaaagt gccctcttat
     5821 ctggagatca gaaaagatct gccacgtaat tgctcgggaa aaataattag aaagaatctt
     5881 aaataaatga aacggcaggg aactaccctg ccagctaata atcacgctct aaaacaatat
     5941 gcattttttg cagggatgtt gtcgtccctg aaaaagcaaa aatggagaaa aggaatgagt
     6001 gaatcattac atctgacccg caatggatca attctggaaa ttacccttga tcgtccaaaa
     6061 gcgaatgcta ttgatgcaaa aaccagcttt gaaatgggcg aagtatttct aaatttccgt
     6121 gacgatccgc aattacgtgt cgccattatt accggtgccg gagagaaatt cttttccgcg
     6181 ggctgggatt taaaagcggc agcagaaggc gaagcaccgg atgctgactt tggcccgggt
     6241 ggttttgcgg gattaaccga aattttcaat ctcgacaaac cggttatcgc agctgtgaac
     6301 ggctacgcct ttggcggcgg tttcgaactg gcgctggcgg cagattttat tgtttgtgcc
     6361 gataacgcca gcttcgccct gccggaagcc aagctgggca tcgttcctga cagcggcggt
     6421 gtgctgcgtc tgccgaagat cctgccgcct gccatcgtca atgaaatggt gatgaccggc
     6481 agacgaatgg gcgcagaaga ggcgctgcgt tgggggatag tcaaccgcgt ggttagccag
     6541 gcggaactga tggataacgc ccgcgaactg gctcagcagc tggttaacag cgccccgctg
     6601 gcgattgcgg cgctgaaaga gatctaccgc accaccagcg aaatgccggt agaagaagcg
     6661 tatcgctata ttcgcagcgg cgtgttgaaa cactatccat cggttctgca ttcggaagat
     6721 gccattgaag ggccgctggc gtttgccgag aagcgcgatc cggtgtggaa aggacgttaa
     6781 caaccgtgag ctattacgcc tttgaggggt taattccggt ggttcacccg acggcgtttg
     6841 tccatcccag tgccgtcttg attggcgatg tgattgtggg agccgatgtc tacatcggcc
     6901 cactcgcctc actgcgtggt gactacgggc ggttgatcgt gcaagcggga gccaatattc
     6961 aggatggctg cattatgcat ggctactgcg acaatgacac catcgttggg gaaaacggcc
     7021 atatcgggca cggagcgatc ctgcatggtt gtgtgattgg tcgcgatgca ttggtcggga
     7081 tgaacagcgt gattatggat ggcgcggtca ttggcgaaga gagcattgtt gccgccatga
     7141 gctttgtcaa agcgggcttt cgcggcgaga aacgccagtt gttgatgggt acgccagccc
     7201 gcgctgtacg cactgttagt gacggcgagt tacactggaa acggttgaat accaaagagt
     7261 atcaggatct tgtcgggcgc tgtcatgcat cgttacatga aacacagcca ctgaggcaaa
     7321 tggaggaaaa tcgcccccgt ttgcagggga cgacggatgt gacgcctaaa cggtgatctt
     7381 gccggatgcg gcgtaaacgc cttatccaac tctgctggag agcaaccgta ggccggataa
     7441 gatgcgccag catcgcatcc ggcgaccagg tcgcacggta tcatcgaaat gacattaacg
     7501 acgcatactc tttgacaaca tcatctgcca cttctgctcg cggttaagtt ccgtgagcgg
     7561 aacggcagga atacgcggtg tctttcttac gccgactaat ttttcccgac tgttattgcg
     7621 caggcgcgcg taaatttgct catcgatatc gaccacttta accagtcgct gacactggca
     7681 tccccgccct tccaacttat tagggatcat cttaacttcg cagcttattt ctgtgacttc
     7741 cgacagaata taagtcaggg tattaaccgc cttgctgtgt tcaatatcga aatgaataga
     7801 gatctctctt gctatcaccc accgattttc agccatcatc cattcggcga ttaacaagta
     7861 gagtggtttt tcaacatatc cttcacacat acgaactcca gtttcacaaa ttaaaagtct
     7921 ggctatgact cgttttgaag caattaaaca aggccatatt aaaattgtgg atatatcgat
     7981 agtatgcaac ttcaccgtcg ataagtgtga gctaaatcct gcttatgtca tcaaaaatat
     8041 cgattcccca aaagatttac ttaatgggca aaaaaaacgg tcctcatcag agaaccgtat
     8101 tagttattcg atcaaattag cggatgaaaa atatccgcca tgacacgcta ttatttgatc
     8161 tgcgcgtgca tttcctgcac cgaaattact ttttcagtcg catcggcatt cagcgccatc
     8221 gcggtagcga aaccaccgtt cagggtggtg tcataatgca ctttatattg cagcgcactg
     8281 cgacggatca cgcgggagtc ttcaatcgca cgacggcctg aggtggtgtt gatgatgtag
     8341 gtatattcgc cattcttgat acggtcctga atgtgcggac ggccttcatg caccttgttt
     8401 accagacgcg gattgatacc cgcttcgccc agcacaatcg ccgtgccgtg ggtcgcatcc
     8461 agctcgaagc cctgtttcag cagttttgcc gccaggtcca ccacgcgttc tttatcgcct
     8521 tcgcgcacgg aaagcagcgc acgaccgtgt ttcttcatgg tggagttgct gcccagctgc
     8581 gctttggcaa acgcttcagc gaaggtgcgg cccacgccca tgacttcccc ggtagagcgc
     8641 atttctggcc ctaacagcgg gtcaacaccc gggaatttat tgaacggcag caccacttct
     8701 ttcaccgagt agtacggcgg gataacttct ttggttacgc cctgctcagc cagcgatttg
     8761 ccagccatca cacgcgccgc cacttttgcc agcggtacgc cggtggcttt ggagacgaac
     8821 ggaacggtac gcgccgcacg cgggttaact tcaatcaggt agacttcgtt gtttttcacc
     8881 gcaaactgca cgttcatcag accgcgcacc tgcaattcga aggccagttt ctgcacctgc
     8941 tggcgcatca catcctgaat ttcctgactt aaggtgtagg ctggcagaga acatgcggag
     9001 tcaccggagt gcacgcccgc ctgctcgatg tgctccatga tgccgccaat aagcaccatt
     9061 tcgccgtcgc agatggcatc cacgtcaact tctaccgcgt catcaaggaa gtgatccagc
     9121 aacactggcg catcgttaga cacgctgacc gccgtctgga agtagcgacg caggtcagct
     9181 tcgtcataga cgatttccat cgcccgaccg ccgagaacgt aagacggacg taccaccagc
     9241 gggtagccaa tctctttcgc cttctctacc gccatttcaa tagcggtaac ggtggcgttc
     9301 gccggttgtt tcagtttcag acgctcaacc gcatgctgga agcgttcacg gtcttctgca
     9361 cggtcgatag catccgggct ggtgccgata accggtacgc cagcagcttc cagcgcgcgc
     9421 gccagtttca gcggggtctg accgccgtac tgaacgataa cgcctttcgg cttctcaata
     9481 cgcacgattt ccagcacatc ctccagagtt accggctcga agtagaggcg atcggaagtg
     9541 tcgtagtcgg tggagacggt ttccgggtta cagttaacca taatggtttc gtaaccgtct
     9601 tcgcgcagcg ccagcgaggc gtgtacacag cagtagtcga attcgatacc ctgaccgata
     9661 cggttcgggc cgccgccgag aaccatgatt ttttcacggt cggtagacgg attcgcttcg
     9721 cactcttctt cataagtgga gtacatgtaa gcggtgtcgg tggcgaactc tgccgcacag
     9781 gtatccacgc gcttataaac cgggtgcagg tcatactggt cgcgcagctt gcggatttcc
     9841 gcttcgcgca cgcccgccag ttttgccaga cgcgcatcgg caaagccttt gcgtttcagc
     9901 tggcgcagga attcagcgtt caggccagtg atgcccactt ccgctacttt ctcttccaga
     9961 cgcaccagtt cttcaatctg taccaggaac cagcggtcaa tattggtcag gttgaacacg
    10021 ccgtccacag acaggcccgc acggaaggca tcggcgatgt accagatacg ctctgcgcca
    10081 gcatctttca gttcgcgacg gattttggtt aacgcttccg ggtcatcgag gctcactttc
    10141 gggtcgaatc cagtcgcacc gacttccagg ccgcgcagcg ctttttgcag ggattcctgc
    10201 tgcgtgcgac caatcgccat cacttcgcca accgatttca tctgagtggt cagacggtcg
    10261 ttagcaccag cgaatttttc gaagttgaag cgaggaattt tagtaaccac atagtcgatg
    10321 gacggctcga aggaggccgg agtacgtccg ccagtgatgt cgttcatcag ttcgtcgagg
    10381 gtgtaaccca ccgccagttt cgccgccact ttagcaatcg ggaaaccggt cgctttcgac
    10441 gccagcgccg aagaacggga cacgcgtggg ttcatttcga taacaatcag gcgaccgttt
    10501 ttcgggttca ccgcaaactg gacattggaa ccaccggttt caacgccgat ttcacgcagc
    10561 accgccatcg aggcgttacg catgatttga tattctttgt cggtcagcgt ttgggctggc
    10621 gcgacagtga tggagtcacc ggtatggatg cccattgcat caaagttttc gatagagcag
    10681 acgatgatgc agttgtcgtt tttatcacgc accacttcca tctcgtactc tttccagccg
    10741 atcagcgact catcaatcag cagctctttg gttggggaga gatccagacc gcgggcgcaa
    10801 atttcttcaa actcttcgcg gttataagcg ataccgccgc cgctaccgcc catggtaaag
    10861 gatgggcgaa taatgcacgg gaagcccacg tcagcggcaa ccgccagcgc ttcttccatc
    10921 gtgtgtgcga taccggaacg cgcggtttcc agaccaattt tcttcatcgc tacgtcgaaa
    10981 cgacggcggt cttctgcttt atcaatcgca tcggcggtcg caccaatcat agtcacgccg
    11041 aactcttcca acacgccctg acgctccagc tccagcgcgc agttcagcgc cgtctgacca
    11101 cccatcgttg gcagcaccgc gtccgggcgc tctttttcaa taatcttgcg tactacttcc
    11161 cagtgaatcg gctcgatgta ggtcgcatcg gccatttccg ggtcggtcat gatggtcgcc
    11221 gggttggagt tcaccagaat gacgcggtaa ccctcttcgc gcagggcttt acatgcttgc
    11281 gcgccagagt agtcaaactc acacgcctga ccgataacaa tcgggcccgc acccagaatc
    11341 aggatacttt ttatatctgt acgttttggc atggctcttt tactcctgat taattagcgg
    11401 ttttacggta ctgctcaatt aactcgataa agtggtcgaa caacggcgct gcgtcgtgtg
    11461 gcccagggct ggcttccggg tgaccctgga agctgaacgc cggtttatca gtgcgatgaa
    11521 tgccctgtaa cgtaccgtcg aacagggatt tatgcgtgac acgcaggttt gcaggtaatg
    11581 ttgcttcatc caccgcaaaa ccgtggttct gggcggtgat catcaccacg tttttctcca
    11641 catctttaac cggatggttg ccgccgtggt gaccaaattt cattttgaca gtcttcgcac
    11701 cgctcgccag cgccagcagc tgatgaccga ggcagatgcc gaataccgga atatcggttt
    11761 cgaggaattt ctggatggcg gtaatggcgt aatcgcacgg ggccgggtcg ccaggaccgt
    11821 tggagaggaa gatgccgtct ggattcattt tcagcacatc ttccgcagaa gtttgcgccg
    11881 gaacgatggt caggcgacag cctctatcca ccagcatccg caggatgttg cgcttggcac
    11941 caaaatcata agccacgacg tggaacggca gctcgtcttc ttttttcgct tctggcaggc
    12001 cgccggtcaa cgtccagctc ccttgtgtcc agctataggc ttctgcggtg gtcacttctt
    12061 ttgccagatc catgccattc agacctggga acgcgcgggc tttttctaac gccaacgccg
    12121 catccgggtt atcgcctgcg ataatgcagc cattctgtgc gcctttctcg cgcagtaaac
    12181 gcgtcagctt acgggtatcg atatcggcaa tcgccacgat gttatggcgc ttcaggtaag
    12241 aagagaggtc ttcggtatta cggaagttgc tggcaatcag cggcaggtcg cgaatcacca
    12301 gaccttgtgc atgtacctga gaagattctt catcggcgtc attagtgccg acattgccaa
    12361 tatggggata agtaagagta acgatttgac gagaatagga aggatcagtg aggatttctt
    12421 gataaccggt cattgaagta ttgaaaacga cttccccaac cgccgaacct gttgccccta
    12481 tggcccgacc gtgaaactgg gttccgtctt ccagaaccaa tagcgctgac ttaatcaaaa
    12541 caccctccag agaatattca ctcactttat ttgcatatta attcacaatg atgacgtaaa
    12601 tcaatgcaaa tctgcttacc gacgaatttc tggcaaacgg cggcattctg gagatatggg
    12661 cgctaaaagt caacttaatg gcgggatttt ttatattatt ttgtggctca tgcatgtttt
    12721 acaggcatta agcgacaaaa agatcatata acctgttctg gaaaacgctt gcgc
//
LOCUS       DBANUU010000124        12597 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000124 DBANUU010000000
VERSION     DBANUU010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.125"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(159..932)
                     /gene="uxuR"
                     /locus_tag="ACIE68_003630"
     CDS             complement(159..932)
                     /gene="uxuR"
                     /locus_tag="ACIE68_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFX5909887.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSQNTDGPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1147..2607)
                     /locus_tag="ACIE68_003631"
     CDS             complement(1147..2607)
                     /locus_tag="ACIE68_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFX5909888.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2688..3872)
                     /gene="uxuA"
                     /locus_tag="ACIE68_003632"
     CDS             complement(2688..3872)
                     /gene="uxuA"
                     /locus_tag="ACIE68_003632"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFX5909889.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4212..5555
                     /gene="gntP"
                     /locus_tag="ACIE68_003633"
     CDS             4212..5555
                     /gene="gntP"
                     /locus_tag="ACIE68_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFX5909890.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5736..6638)
                     /gene="fimH"
                     /locus_tag="ACIE68_003634"
     CDS             complement(5736..6638)
                     /gene="fimH"
                     /locus_tag="ACIE68_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFX5909891.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVKSIIGVTFVYQ"
     gene            complement(6658..7161)
                     /gene="fimG"
                     /locus_tag="ACIE68_003635"
     CDS             complement(6658..7161)
                     /gene="fimG"
                     /locus_tag="ACIE68_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFX5909892.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7174..7704)
                     /gene="fimF"
                     /locus_tag="ACIE68_003636"
     CDS             complement(7174..7704)
                     /gene="fimF"
                     /locus_tag="ACIE68_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFX5909893.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7714..10350)
                     /gene="fimD"
                     /locus_tag="ACIE68_003637"
     CDS             complement(7714..10350)
                     /gene="fimD"
                     /locus_tag="ACIE68_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFX5909894.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVSFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLCDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIAHGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGIYGTLLEDNDLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKRLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10414..11139)
                     /gene="fimC"
                     /locus_tag="ACIE68_003638"
     CDS             complement(10414..11139)
                     /gene="fimC"
                     /locus_tag="ACIE68_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFX5909895.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESAVKLPSDAG
                     SNITYRTINDYGTLTPKMTGVME"
     gene            complement(11176..11715)
                     /locus_tag="ACIE68_003639"
     CDS             complement(11176..11715)
                     /locus_tag="ACIE68_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5909896.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAEACR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11780..12334)
                     /gene="fimA"
                     /locus_tag="ACIE68_003640"
     CDS             complement(11780..12334)
                     /gene="fimA"
                     /locus_tag="ACIE68_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFX5909897.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALADTTPTTVNGGTVHFKGEVVNA
                     ACAVDAGPVDQTVQLGQVRTATLKQVGATSSAVGFNIQLNDCDTTVATKAAVAFLGTA
                     IDSTHPKVLALQSSAAGSATNVGVQILDRTGNELTLDGATFSAETTLNNGTNTIPFQA
                     RYFATGAATPGAANADATFKVQYQ"
BASE COUNT         2988 a         3300 c         3077 g         3232 t
ORIGIN      
        1 aattatattg gtaaatagcc actgagaaga atgagtactc acgttcaggt tatacagata
       61 gctaaacaat accctgcaat ttacaaagta atactcattg cgacttctta atttaagaaa
      121 gcgcaaggaa cgtttaccct tgcgcttatt ataataagtc aggcgtcgac tttatccagc
      181 ggccatgaat caaacagata gccgtcaaaa taaatatcgt caacgttcga gaattccagc
      241 agacgttgct taacgttttc cagatgctgc cacattgcca gcttcgccgc tcgggcatct
      301 tttttgatta acgcggcgag gatctgtttg tgatcgccca accactcttt gcgatacagg
      361 ctgtcatcca gatggctgtg caactggatc cacatcggat tgttttcccg ccactgccag
      421 gactgacgga acagctccac cagcatgctg ttatgcgtcg cttcggcaat ggcgagatgg
      481 aattgcatgt caccactttc gctgctgccc ggcgcgctgg aagccagttc acgctcttcc
      541 agttgcaatg cctgacgcat tttgacgata tcttcgcggg tagcctgcag ggcggcaaat
      601 tccgcgatgt tactttccag taactgacgc gcctgtaaca gctcaaaagg accggcatca
      661 ttgcagacgt tggcatccgg accgtctgtg ttctggctgc ctgagctatc aagaacatag
      721 ataccggcac cccgacgcac ttccaccagc cctttgatct ccagcatgat cagcgcttca
      781 cgtaccaccg tccgcgtgac atcaagcatt tctgcaattt cacgctccgg cggcagccgt
      841 tcgccagggt tgtacggcgt ctttatgatc agatcgcgga tcatcgcccc gacttcctgg
      901 taaggtcttt gcgcagaggt ggcagatttc atggtgttgt gtccgttagg tgcggttaat
      961 caaatttttg ccggatgcgg cgtgaacgcc ttatccggcc tacaacgggc aattttacgg
     1021 cccccgttaa tctcaaacta taccattaac actggaagtt ggcacgtaca ggtgggcaaa
     1081 aaaacgaaaa ctggtcaacc aaatatcagt ttgattgacc agtaagtctg tagggtaatc
     1141 agttagttac agcgcagcca cacactcgcg cgcaccgcgt tcgcacagct gctgatatgc
     1201 cgccgtcacc gcgccaacaa agtcggcatt ctgcggcaga tcatcggcaa aaataccgct
     1261 caggcccagc agcgctttca cgcggtctgc gccctgatac tgcgcgttga tcttctggaa
     1321 ctccgccagc atcgggtcga ccacgtcaat ggcattaccc tgctcatcca cgccctgggt
     1381 gtaacgcatc cagccagcca cgcccagcgc caggtgacgc cagctgccgc cgttttgcag
     1441 gtgcagacgc accgggtcca gcagacgctg cggtaacttc tggctgccgt ccatcgcaat
     1501 ctgccaggta cggtgacgca gagacgggtt gctgaaacgc tcgatcagca gcgtcgcata
     1561 ggcgttcagg tctgtacctt ccggcatcga cagcgttggc gcttgttcct gcatcatcag
     1621 ggcaaaggcc gctttgcgat aagccgggtt agtcatggtg tcggcaatgg tttcatagcc
     1681 gccgaggtaa ccgaggtacg ccagaaaaga gtggctgccg ttcagcatac gcagcttcat
     1741 catttcgaac ggcacaacgt ctgcaacgaa ctgtgcgccc actttatccc agtccgggcg
     1801 accattaacg aagttatctt caatcaccca ctgacggaac ggttcgcagg caatggcgca
     1861 cgggtcgtaa acacccagct ggtcagcaat ttcctgtaag gtttctggcg tcgccgccgg
     1921 aacgatgcgg tcaaccatgg tgcacgggaa ggtgacattt tcttcaatcc atgccgccag
     1981 ctgcgggtca cgcgcctgag ccagccccag taccgcgacc ttcgccacat gaccgttttc
     2041 acgcacgtta tcgcaggaca tcaccgtaaa cgctttcaac cctttttcac gacgcagacg
     2101 caaggcttcg acgatgtaac caatcgcgga cttcggcgca gtcgggtttt ccagatcgtg
     2161 cttgatcagt gggttattga gatccagttg accgcttgcc gcatcagcgc agtagccttt
     2221 ttccgtgacc gttagagaga caatcgccgt ttgcggacgc gccatcgcgt tgagaatacc
     2281 ttcgcagcca tcgatttccg gatgcagcgc ttctttcatc gaaccgataa ttttcagctc
     2341 ggtgctctct gcgccttttt ccgctacggt gtacagcagt tgctgttttt tcaggttttc
     2401 gatcagcacg cggtcgttgc ctggcatcag gttaacttcg cagatgcccc agtcgctgtc
     2461 ggtgctttcc agcagatgat gggtatacag cgcctggtgc gcgcggtgaa acgccccgca
     2521 accgagatgc acaatgcgtg attccagacg agaatgatcc catgacgggc gggcaaccgg
     2581 cagattgctg tcaacaatag tagtcatcgt aaactccaga atccgtcttc aaagagtgac
     2641 ggcaaggaag aaggaaccgg cacggcgcgc agccatgccg gtggatatta acggctaaag
     2701 aaagcgcgct ggatcgccag ttcgacaccg cgaacttcgg ccaggccttt cagacgacca
     2761 attgcggagt aacctgggtt ggttttcttc ttcaggtcgt ccagcatctg atgaccgtgg
     2821 tccggacgca tcgggatcag gtcttctttg ccttccgctt tacgacggtg ttcttcttca
     2881 acaatcgctt tcaccacttc gtacatatca acgtcaccgt tcaggtgcgc cgcttcgtgg
     2941 aaggttttcg ggttatcttc acgcatggtg gagcgcagat gggtgaagta aatacgcgga
     3001 ccgaactgct tgatcatatc aaccagatcg ttgtcagcac gcacgccgta ggaaccggtg
     3061 cacatggtaa aaccgtttgc catgctgttt acggtatcaa ccatccactg catatcttca
     3121 atggtggaaa caatgcgcgg caggccgagg atcgggcgcg gcggatcgtc cgggtgaaca
     3181 gccatacgca cgccgacttc ttcagcaact ggaataatcg ctttcaggaa gacggcaaag
     3241 ttttcgcgca gcttcgcttt gtcgatatct ttgtacagct ccaggtgttt acggaactgg
     3301 tcgagggtgt agccttcttc cgcgcccgga agaccagcaa tgatgttacg ggtcagacgc
     3361 gctttgtctt cgtcgctcat agtggcgaag cgaacagcag cctgagcaat ttcttcttcg
     3421 gtgtaatccg cttccgcgcc tggacgtttc aggatatgca tttcgaatgc agcgaattcg
     3481 atctggtcga agcgcagcgc tttggagccg tctggcagca cgtattcgag gtcagtacgg
     3541 gtccagtcga gcaccggcat gaagttgtag cacacggtgc gaatgccgca ctgcgccagg
     3601 ttgcgcagag tctgctgata gttagcgatc cactgctcat agttgccagt gtgggttttg
     3661 atatcttcgt gaattggtac gctttctacg acagaccaca ccaggcctgc gtcttcaacg
     3721 atcgccttgc gtttgaggat ctcttctacg gaccatactt cgccgttcgg gatatggtgc
     3781 agcgcggtaa ccacgccagt tgcgcccgcc tgacggacat cagctaaaga aaccggatcg
     3841 tttgggccgt accagcgcca ggtctgttcc atgatttttc ctctttcaca tagtgggttc
     3901 cacagcaaag agacgcggaa ccctggagtt gtttaggtat ccggatgaac aaccagaccg
     3961 cagccgtggc gttgcatcgg atgagatggc gtataagttc taccttttgg taagacatgt
     4021 caacccgaat tctgaaattg gttaaccaca tcacaagaat ttcactctca tggttgttgc
     4081 tgcaaattgc agcaaagggt aatcgatggt tattgatcag aggatgtgac attcatcgca
     4141 acaatggttg accaatttac ataacatgtc gcccaaaata acaacggttc aaccaggtaa
     4201 ggtaaaacgt tatgcatgtg cttaacattc tctgggtggt attcggcatt ggcctgatgc
     4261 tggtactgaa tttgaagttc aaaatcaatt caatggtggc tttgttggtg gcggcgctgt
     4321 ccgtcgggat gctggcgggc atggatttga tgtcgctgct gcacactatg aaagcgggct
     4381 tcggcaacac gctgggggaa ctggctatca tcgtggtgtt cggtgcggtc atcggtaaat
     4441 tgatggtcga ctccggcgcg gctcaccaga tagcgcatac gctgctggcg cgtctcggtc
     4501 tgcgctatgt acagctgtcg gtgattatca tcggcctgat ttttggtctg gcgatgttct
     4561 atgaagtggc ctttatcatg ttagcgccgc tggttattgt tattgccgcc gaagctaaaa
     4621 ttccgttcct gaaactggcg atcccggcag tagcagctgc cactaccgca cattcactgt
     4681 tcccaccgca gccgggtccg gtggcgctgg tgaatgctta tggcgcggat atggggatgg
     4741 tttatatcta tggcgtactg gtgacgatcc caagtgtaat ctgcgcaggt ctgatcctgc
     4801 cgaagttcct cggcaatctt gagcgcccaa cgccatcatt cctgaaagca gatcaaccgg
     4861 tagatatgaa taatctgccc tctttcggcg tttcgattct ggtgccgctg atcccggcga
     4921 tcattatgat ctctaccacc atcgccaata tctggctggt aaaagatacc cctgcctggg
     4981 aagtggttaa ctttatcggt tcctcgccga ttgcaatgtt tattgcgatg gtggttgcat
     5041 tcgtactctt tggcaccgca cgtggtcatg acatgcagtg ggtgatgaac gcttttgaaa
     5101 gcgcggtgaa gagtattgca atggtgattc tgatcatcgg tgcgggtggt gtgctgaagc
     5161 agaccatcat cgacaccggc attggcgaca ccatcggcat gttgatgtcc cacggcaata
     5221 tctcgcccta catcatggca tggctgatca ctgtgctaat tcgtctggcg acgggtcagg
     5281 gtgtcgtttc ggcgatgacc gccgccggga ttatcagtgc tgcaatcctt gatccagcaa
     5341 ctggtcagct ggttggcgtg aatccggcgc tgctggtact ggcgacggct gcgggttcca
     5401 acaccctcac ccacattaac gatgcttctt tctggttgtt caaaggatac tttgacctgt
     5461 cagtaaaaga cacgttgaaa acctggggtc tgctggagct ggtcaactcc gtggttgggc
     5521 ttattattgt gctgattatt agcatggtag cgtaaaagaa caccagagcc tgccaatggc
     5581 aggctctgtt cggattgtcg gcaaagtacg ctttgtttat gccagatgcg acgctgacgc
     5641 gtcttatctg gcctacaaag ggctaacgtg caggttttgt aggtctgata gcgtagcgcc
     5701 tcaggtacca gcattagcaa tgtgctgtaa tttttttatt gataaacaaa agtcacgcca
     5761 ataatcgatt tcacattccc tgcagtcacc tgccctccgg tacgtgcgta atttgccgtt
     5821 aatcccagac ttaccgccga agttcctact gctcctaacg ataccgtgtt attcgctgga
     5881 ataatcgtac cgttgcgcgt caactgtacg ccgacgccct gcgctggtga aaacgacgcg
     5941 gtattggtga aaatcgagtt gcccgcatct gcggttgtgc cggagaggta ataccccagg
     6001 ttttggcttt tcgcacaata aacggtaaga ggaattggca ctgaaccagg gtagtccggc
     6061 agagtaacgg tgacatcacg agcagaaaca tcacagccgc cagtgggcac caccacatca
     6121 ttattggcgt aaatattcca cacaaactgg aaatcatcgc tgttatagtt gttggtctgt
     6181 cgcaaaataa gcacggcaat taatgagcca gctttaatcg ccaccccgcc cgcactgctc
     6241 acaggcgtca aataaagcgc caccggccac ggcttatccg ttctcgaatt ataaacaacg
     6301 cgcggcgttt cgctggtggt aggaaatgga tagctactgc cactatattt tacggtcccg
     6361 gaaaaattag ataacacgcc gccataagcc gagcctcgtt gcagtgtgac atagtctgta
     6421 atggtttccg gataatcgtt atggcaaaag atttgcgtcg aaagatctac gaccaggttt
     6481 tgccccacat tcacggcggg cgcaaggttt acataaacat tagcgctgcc accgccaata
     6541 gggatagctg taccattggc ggttttacag gcgaatgacc aggcatttac cgaccagccc
     6601 atcagcagta cagcaaacag ggtaataact cgtttcatta caatcatctc ttcgggttca
     6661 gctgtaggta taggtgatgc taatcactgc ctgaatggtt ccctgagtgg ctccgccatt
     6721 taccgtcaat gctctgacct gtaacgggaa gtgcgctgat tgtgaggaat catccacctg
     6781 aactgttttg gttgcgccag tattcaatgt gttgccactg tcatcctgta gctctaactg
     6841 gatgttttgc gcggtcccct gatttttata atatccggta ctgtcggctg ccccgctgaa
     6901 gctggcagtg accctcgacg ttcccaccgg acaattagtc aactcaagcg caacatcatg
     6961 ccaggccgat gccgccccgg cagacataag actgaaagaa taaagatcgc cgagatcaac
     7021 cgtggcattg gtggtggaaa ccgtgcacgg tttagcgacg accttaccgt tcaccgtgat
     7081 ggtgacatcg gctgcctgta tcgtcgcact tgcgagcgcc aatattgccg ccaataaata
     7141 cccacgtttg cgccatttca tgagcatctc cagttactga tattcaagag tgaaggtagc
     7201 cgtggcattg atatgccccg cagtgacagg cacctgtgtc gccattagcc gggcgtaaaa
     7261 attcagcgta tttggtttac ccggcgtcag ggtcgtccac gaaatcgcgg acgatggggc
     7321 attaaggggt atctgatttt gttgctcatt cagaagctgt attcccagtc ccgcagccgc
     7381 tgacaccgta ttttcaagtg caagcaggtt ggcattgtgg ctatctgcaa cgccagtaaa
     7441 cccaaccttt acggcagaaa cggcgttacc acagggtgac agcaaaatac gaaatggaac
     7501 gacaggagtc gtcgcgccaa tgttgttaaa ttgcttcgcc gcgttttcca tcagatcaac
     7561 agtaaaattg gttgattcag cggccacact acagccatta tctctgacat agccgcggat
     7621 agtaatcgtg ctatccgcag ccaaagcgtg acttaccgcc agccacaaaa aagcgcaaag
     7681 aagataaaaa ggtttgtttc tcatcacgcc cccttaacga cattcagctg atagctgggt
     7741 taataactgc tgctgactct ctggtggcag ttgataattg gcgacacagt gagcattttc
     7801 ctcttctccc catttcacct gcacttttcc cgctaaaggc attccgctga ggtaaacctg
     7861 accattatcc gcaacaatgc cgctactctg gctactctct gatgtcacca tcgccccaaa
     7921 cggcagcggc ttattattgt gggttagcgt catgagcagt tttatcccaa cgcgcgcttt
     7981 aaactctgct cgcacgatcg ccccacgggt gggaacaacg ttagcgaccg cattatctaa
     8041 atcgacgtta tcagccaggg tattggtatc cagcgccact ctgttttccc gatattcagt
     8101 ggcataaggc agcacggcat aaccccgcca gtcggtacgc actcctgtct ggttttcgac
     8161 ttttgcatct tttgcgccag gcgctttaac aagcaccacc gtatcgttta acggctgccc
     8221 cagcgttacg ccattggcat gagccagtac cccaccgctg actccgtaat agagccgctt
     8281 aatatcatcg ctatggctgt aaccgatatt ggcattgccg taaccaccgc gataattcag
     8341 cgtggcgtag cctgtacttc cgctatttcc atcgcctccc ccggcataac cggtttgcac
     8401 gctatagctg aggtcgttgt cttccagcaa cgtaccgtat ataccggcca gattggtcat
     8461 ccgaccgttg agatcgtgtg acatgctgta gctggcactg gcatgtcgcc actgagattt
     8521 actgtcagaa cgcagccagt ggctgaaagg aatattgacg ttaagcgcta acatctgatc
     8581 ccgtcctttt tgccaggcgt ttttcgtcag gctatagctg agcgtccagt taatatcttc
     8641 gaacgcagta tttaatccag cctggaattg ctcatcgaca ttactcgttc cccaataagt
     8701 ttgatggcta ccactcaaat acagtgttga tgtgcgcccg agttgctgag taacggtgag
     8761 ttgtaatttc ccgcgtttgt tataagcgag attgtaatag tcggtgaatt tcggcttaac
     8821 ctgaataact ccatcctgtg tttcgatgtt gtagccattc attcgactgt atgttgtatc
     8881 agcgaaatta aaatacccac tggtcgaata acggtaaccc actaactgaa tattcgtgcc
     8941 tgactcattg agcgatttgt tataaagaaa acgcaccgat tgtccgtcat gctgactgtc
     9001 atcgggaagt gtggaattag cctgcgtcat atccacagac agagcgccca gtgcccccat
     9061 atttttcccg ataccgaaat taaacgcacg ataacgatcc gccagttgcg ttccaccata
     9121 tattgtccag ccagccggaa ggccgtggag taatgtactc tggaaaaagc ggggtttttc
     9181 ctgctgcgca tttccactac ggtattctcc tgccgtaatg gaataacgtg tatgcccttc
     9241 acgctgcaaa agcgggactg acgaataggg tacggtaaaa atctgcgtac tgccgtcagc
     9301 ctctttaatc gttacctgca agtcaccact attacctgcg gcatagatat cgttgatggt
     9361 aaaaggcccc ggtggcaccg tactattata aatgtcatac ccattttgtt taatagtgac
     9421 ctgtgcagta ccatgagcaa taccgtggat caccggggca aatcctcttt ggctatcggg
     9481 taacatattg tcatctgagg ccaattgtgc gccgcgaaag ttaataccat cgaaaatatc
     9541 gccctgagta taaccatcac ccagcgtcag ccgggaacgt aacggaatta tgtctcgctc
     9601 aagccaggta ttgatatgct gccatttatt tttgctacct gatgaactgt cgctactgtt
     9661 ataactccag gtggtattgt cgcataaacg ccacgcacca atatttaacc cactctgtag
     9721 gtttaaatat gcataatggc tgttaccccc aattcgattc tgtacactat ttccgctgaa
     9781 attataattg agcaatccgg cattaatacc gggatcccat aactcaggag gaatataacc
     9841 acgcgcgcga ttactcataa atgcctgagg gatcgtcagg ttgagtcgct gctgaccaac
     9901 atctaaatgc gcagtagcgt catggatcat tgaggttaat ggcacgcagg catcatccgc
     9961 cagcagattc ataccggaga cagaagccgt attcagcccc atactggcaa gttgcgcgcg
    10021 tgtcaggcag ggaacaatcc cttgttcgct gtcgcccgta ttaaatgaga catcacgcgt
    10081 tgccatataa ccattattca aatagatatc gacacgatac gtccctggcg gtaattcttg
    10141 cccattctca aaacgcgata aatcagccac agcctgggga tcatccgcta aaaaacgcgg
    10201 gttaaaatag agttcggcag atgacaaagg tgcctgtgcg gcaaaagcac aggcaacaaa
    10261 gagccggaca aaaaaaccag ccaaacgatg cttacgaata tgcaagcact gtgtgtttcg
    10321 ctggtaaagt cttaaattca gatatgacat tattcctcca gtttaaatca cccggcagtc
    10381 aattcttttt attcaggcga aaatccccct gcgttattcc attacgcccg tcattttggg
    10441 ggtaagtgtg ccataatcat ttattgttcg gtaagtaata ttgcttcctg catcagaagg
    10501 caatttaacc gcgctttcgc ccattggagg caccaatgca ttttcaagaa cccgggttcc
    10561 ggcattcaac tctgttaccg tcaggtaata gggtgtcggg ttaatcagcg tcagagaatt
    10621 cgcgctacga cgaaatctta atttttctgc ggcctgatcg ggtggcaacg ctaatttagc
    10681 cggacgatag tacagtttaa tgcggctgat aattgcgagc tgtagcgtat tctcagtcaa
    10741 ttttgattta tccattgacg gaatcgcttt aacattcatc cagaataaac tttcccgatc
    10801 ctgtggcaat tggttatttg ttgcatcaag aatacgcaag gtattctctt tttttccctt
    10861 catcgcaaac agaggtggcg tcacgataaa acggccatcc tttacaccat cagcattttc
    10921 tacccatgat tgaatcaaat aggtgctatt ttcatcatta tttgtcacgg caagttgcac
    10981 ttgtttttgc cctgccggat aaattacgcg agtcgcacct aaggccactc ccgcttcagc
    11041 gcgtccggca accatcattg ccatgaacat caggatacct gccagcaagc agaatgttat
    11101 ttcctgcgat ttccttacat tgacgttttt attactcact gtcctgttcc tgttatcaca
    11161 ttatctgctg aacaattact gataggttaa agagaaccaa gcctgggcat tggcgatgcc
    11221 gccagtaacc cgacgcccgg tagcacgata tttggcgatg aaatgtagcg aagtagagcc
    11281 tgagtaaagc cgtttccagt ttgctggagg acggttaatc ggtacgaggt ttccttcatc
    11341 atcaaacaac gctacgccaa tattggtggc tatccctggc ccctcaccca cggaaagcac
    11401 atccggattt ttaccatccg cgacaccgtg aaacgccaca cccacacgtt cactcaccac
    11461 cgtgctacat tcccgtaaat gaataacaaa aggcaccggt gcgctatctt cccctaccgc
    11521 atgaaaccgg ttactgctga tttgccccat attgaccgtc atttgtttat caccggcttc
    11581 aatccggcaa gcttccgcaa taatgacgcc ctgaaattgc atatttccgc cgggcagcgt
    11641 ggtattccat ttatttccgg ccagagcaaa cattggcagc aacgacgcta acagaaataa
    11701 tctcttcctt ttcatcgtta tttttatcgc acaagggtgg gcatccctgc ccgtaatgac
    11761 gtccctgaac ctgggtaggt tattgatact gaaccttgaa ggtcgcatcc gcattagcag
    11821 cacctggggt tgcagcaccg gttgcaaaat aacgcgcctg gaacggaatg gtgttagtac
    11881 cgttattcag ggttgtttct gcactaaatg tcgcaccgtc cagcgtcagc tcattacctg
    11941 ttctgtccag aatctgcacg ccaacgtttg ttgcgctacc cgcagctgaa ctctgtagag
    12001 ccaggacttt aggatgagta ctgtcaatcg ccgtccccaa gaaggcaaca gcggcttttg
    12061 tggcaacagt ggtatcgcaa tcattcagct gaatgttaaa accgacagca gagctggttg
    12121 ctccaacctg cttcaaagtg gcggtacgaa cctggcccaa ctgaacggtt tgatcaacag
    12181 ggcctgcatc aactgcgcaa gcggcgttaa caacttcccc tttaaagtga acggtcccac
    12241 catttaccgt cgtgggtgta gtatcagcca gagccgctgt agaactcagg gacagagccg
    12301 acagaacaac aattgccaga gttttaattt tcatactgct ttcctttcaa aaaactattt
    12361 ctaaatcgac atgggcagtc gttctgtaca ctttgttttg tcaacgagtt tgactgccaa
    12421 cactgcacag ttttccccca aaagatgaaa catttggggc cattttgact catagaggaa
    12481 agcatcgcgc acaaactttt tcagtttatt tgttggctta atgttctata gttatcttta
    12541 tttgcagttt tttatattgt atgaggtggt ttttggagag aagaatgagg aagttgc
//
LOCUS       DBANUU010000125        12528 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000125 DBANUU010000000
VERSION     DBANUU010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.126"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            2..850
                     /locus_tag="ACIE68_003641"
     CDS             2..850
                     /locus_tag="ACIE68_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
                     /protein_id="HFX5909898.1"
                     /translation="MLWMMLSALPALSYAQVKNIVTGEYSAVSKIITEKETLYPSDKI
                     LLVFDIDNTLLTSGTRIGGDIWYQWQTDKLPLKPDDSQKVPCLYENTISMLYELSPMQ
                     LTEPQLPTMLKQWQQKHTAFALTSRAPDTLFPTLRELKRNGIDFSSSALRKKEDTLPP
                     FEKGKLKRSWLYSQGVFFSSGQDKGVILDYMLDKMGNKYDAIVFIDDGQANIHAMTKM
                     FSKDKWFSTDFTIIHYTRVEDELIKQQGAVITTAQAEKMATDWKTLAGSLATLFPDRN
                     KLCPIK"
     gene            complement(895..2787)
                     /gene="parE"
                     /locus_tag="ACIE68_003642"
     CDS             complement(895..2787)
                     /gene="parE"
                     /locus_tag="ACIE68_003642"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFX5909899.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(2816..3397)
                     /gene="yqiA"
                     /locus_tag="ACIE68_003643"
     CDS             complement(2816..3397)
                     /gene="yqiA"
                     /locus_tag="ACIE68_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFX5909900.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3397..4224)
                     /gene="cpdA"
                     /locus_tag="ACIE68_003644"
     CDS             complement(3397..4224)
                     /gene="cpdA"
                     /locus_tag="ACIE68_003644"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFX5909901.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4249..4671)
                     /locus_tag="ACIE68_003645"
     CDS             complement(4249..4671)
                     /locus_tag="ACIE68_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFX5909902.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(4672..5301)
                     /gene="nudF"
                     /locus_tag="ACIE68_003646"
     CDS             complement(4672..5301)
                     /gene="nudF"
                     /locus_tag="ACIE68_003646"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFX5909903.1"
                     /translation="MLKPDNLPVTLGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5506..6987
                     /gene="tolC"
                     /locus_tag="ACIE68_003647"
     CDS             5506..6987
                     /gene="tolC"
                     /locus_tag="ACIE68_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFX5909904.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7135..7806
                     /locus_tag="ACIE68_003648"
     CDS             7135..7806
                     /locus_tag="ACIE68_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFX5909905.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            7812..8972
                     /locus_tag="ACIE68_003649"
     CDS             7812..8972
                     /locus_tag="ACIE68_003649"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFX5909906.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9010..9798)
                     /gene="ygiD"
                     /locus_tag="ACIE68_003650"
     CDS             complement(9010..9798)
                     /gene="ygiD"
                     /locus_tag="ACIE68_003650"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFX5909907.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGTL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVEGIEMGSLSMLSVQVG"
     gene            9941..10714
                     /gene="zupT"
                     /locus_tag="ACIE68_003651"
     CDS             9941..10714
                     /gene="zupT"
                     /locus_tag="ACIE68_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFX5909908.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(10772..10942)
                     /gene="yqiD"
                     /locus_tag="ACIE68_003652"
     CDS             complement(10772..10942)
                     /gene="yqiD"
                     /locus_tag="ACIE68_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFX5909909.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11203..11856)
                     /gene="ribB"
                     /locus_tag="ACIE68_003653"
     CDS             complement(11203..11856)
                     /gene="ribB"
                     /locus_tag="ACIE68_003653"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFX5909910.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(11960..12108)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12230..12520
                     /gene="ubiK"
                     /locus_tag="ACIE68_003654"
     CDS             12230..12520
                     /gene="ubiK"
                     /locus_tag="ACIE68_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFX5909911.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3185 a         3333 c         3048 g         2962 t
ORIGIN      
        1 cattctctgg atgatgctct ctgctctgcc tgctttatct tacgcacagg taaaaaatat
       61 agttactggc gaatatagtg cggtcagtaa aattatcacc gaaaaggaaa ctctctatcc
      121 atccgataag atattattgg tttttgacat tgataatacg ttattaacga gtggcacacg
      181 cattggcggt gatatctggt atcaatggca aaccgataaa ttgcctttaa agccggatga
      241 ctcgcaaaaa gttccttgcc tgtatgaaaa tacaatcagt atgctttacg aacttagccc
      301 tatgcagctt accgagccac agttaccgac aatgttaaaa cagtggcaac aaaagcatac
      361 cgcttttgct ctgacatccc gtgcaccgga tactctcttt ccgacattgc gggaattaaa
      421 gcgaaacggc attgattttt cgtcttccgc actcaggaag aaagaggata ctttgccacc
      481 ctttgaaaaa gggaaactca aacggtcatg gctgtacagc cagggggtat ttttttcttc
      541 cgggcaagat aaaggcgtaa ttctcgatta tatgctggat aaaatgggga acaaatatga
      601 cgcgattgtt tttatcgatg acgggcaagc aaacattcat gcgatgacga agatgttctc
      661 taaggataaa tggttctcta cggattttac cattattcac tataccagag tggaggatga
      721 gcttattaaa caacaaggtg cagtaataac aactgcacaa gcagaaaaaa tggctacgga
      781 ctggaaaact ctggctggtt cgctggcaac actatttcca gatcgtaaca aactgtgccc
      841 aataaaataa tcaacgacaa cccggcaaaa cacatgtttg ccgggttaag attatcagac
      901 ctcaatctcc gccatgtcgc ctttctcttg caaccagttg cggcgatctt ccgagcgttt
      961 cttcgccagt agcatgtcca tcatcgcgtc agtacgctga tcgtcttcat catcgatagt
     1021 caactgcacc agacggcgag tgttcggatc aagcgtggtt tcacgcaatt gcatcgggtt
     1081 catttccccc agacctttaa aacgctggac gttcggcttg cctttcttgc gttttaattg
     1141 ctcaagtacg ccctctttct cttcttccgt cagcgcgtaa taaacctctt tcccgagatc
     1201 aatacggtag agcggtggca gtgcgacgta aacgtgaccg tgtttcacca acgcgcggaa
     1261 gtgttttacg aacaaagcgc agagtagcgt ggcaatgtgc agaccatcag agtccgcatc
     1321 cgcgaggata cagattttgc cataacgaag ctggctcaga tcgtcgctgt caggatcgat
     1381 accgatcgct accgaaatat catgcacttc ctgcgaggcc agcacttcgt cggaagaaac
     1441 ttcccaggtg ttaaggatct tacctttcag cggcatgatc gcctgatatt cacgatcgcg
     1501 cgcctgcttg gcagatccgc ctgcggagtc accttccacg aggaacagtt cggtacggtt
     1561 aaggtcctgc gcggtacaat cagccagttt gccaggcagc gccgggccgc tggtcagctt
     1621 tttacgcacc acttttttag ccgcacgcat acgacgctgg gcgctggaaa tcgccatctc
     1681 tgccagcaac tccgccgcct gaacgttctg gttcagccac aggataaagg catctttcac
     1741 cacgccagaa acgaatgccg cgcattgacg cgaagagaga cgctctttcg tctgcccggc
     1801 aaactgcgga tcctgcattt tcaccgacag aacataggcg cagcgatccc agatatcttc
     1861 cgccgacagc ttcacgccgc gcggcagaat gttgcgatat tcacagaact cacgcatcgc
     1921 gtccaacagg ccctgacgca ggccattaac atgggtaccg ccctgcatcg ttgggatcag
     1981 gttgacgtag ctttcggtca gcagttcacc gccttccggc agccacagta gcgcccagtc
     2041 cacagcttca gtatcgccag cgaaattacc gataaacggt ttttctggca gcgtcggtaa
     2101 accgtttacc gcttccgcca ggtaatcatt cagaccgtcc tgatagcacc agcgctgttc
     2161 ggtgttgttg atctcatctt taaaggtaat ctcaacgccg gggcacagta ccgctttggc
     2221 tttcagcaca tgcgtcaggc gtgaaacaga aaatcgcggg ctgtcaaaga aggtttcatc
     2281 cggccagaag tgcacgctgg taccggtatt gcgtttaccg caagtgccga caacctgtaa
     2341 atcctgcacc ttttcgccat tttcaaaggc gatgttataa acctgaccat cgcggcgcac
     2401 gttaacttca acgcgcttcg acagggcgtt aaccaccgaa atccccacgc catgcaggcc
     2461 gccagagaac tggtaatttt tgttagagaa tttaccgcct gcatgcagac ggcaaagaat
     2521 caattcaacc gccggtacac cctcttccgg gtgaatatcc accggcatcc cgcgcccatc
     2581 gtcaataact tctaacgact ggtcagcatg taagattaca tccacgcgtt ttgcgtgacc
     2641 cgccagtgct tcatcgacac tgttatcaat aacttcttgc cccaaatggt tagggcgagt
     2701 ggtatcggta tacatccccg gacggcggcg aaccggctca agcccggtga gtacctcaat
     2761 ggcatcagcg ttataagttt gcgtcatggt ttaagttagt aattcgagtt gatcgtcaga
     2821 gatggtgcag accaagaaaa tcgacgatcg ggttgaaata atcttcgaag cccgtgaatg
     2881 cgtggttgcc gccttctatg acagtctggc ggcaggaagc gtagtacgcc accgcctggc
     2941 ggtaatccag cacttcatct cccgtctgtt gcagcagcca gatcaaatcc ggcgcttcca
     3001 gcgggtcaat ctgcatgact ttgagatcgt aaatatggcg tgactctagc acatattgct
     3061 gcccggtgta ggggttctcg ttctgaccga gatagtccgt cagcagttca aacgggcgca
     3121 ccgccgggtt taccaccact gcgggcagca taaaacattg tgacaaccag gtggcgtaat
     3181 atccccccag tgacgaaccg acaataccca gcgaatcgcc gccatgttcc aggacaatgg
     3241 attccagcag ctctgccgcg tcggaaggat acggcggcaa ctgcggaatg atcatctcaa
     3301 cgtcagggtg atgttccgcc agccagtttt ttaacaagct cgcttttgca gagcgcggcg
     3361 agctgttgaa accgtgtaaa taaagaagcg tagacatcag tagccttctg aagcggtatc
     3421 aggttggaaa cgtgtgtccg ccaggcgatg cacctcggtg gtcagcgtgc catcagcatg
     3481 taactcgaga gtacgccagc cgggcgcgat agtatccagc gtaaagttgg aacagtgcgg
     3541 cttaaactgc acacaggtcg acggcgttgc cagcaggcgg cgaccgttcc agtcgagatc
     3601 cagctcctga tgaatatgac cgcacagcaa gtatttgacg tgcggaaact tcgccagcac
     3661 ggtatccagt tcgcccgcgt tacgcagact gtgttgatcg agccaactac aacccgcagg
     3721 tagcggatga tgatgcagca gcagcaacgt atggcgttct ggcgcatcgg ccagtttacg
     3781 ttccagccac tcaagctgaa actcgctcag ctcaccgtgc ggcacgccaa acacctggct
     3841 atccagcaac aggatttgcc attgctcacc aataaacacc cgcttcgccg gggagatacc
     3901 cgcatcctgt aacgcgctgt acatcgcggg ctggaaatcg tggttgccag gcagccagac
     3961 gcagggcgca cgaaaacttg cgatgccttc agcgaaatgc tgataggccg cagaggattg
     4021 atcctgcgct aaatcacctg tcgcgacaat caggtcgaat tcgtgctggt gtggacgaat
     4081 cgcctccagc accgcctggt agctttccca ggtatttacc cctaacaggg cttcgtgctt
     4141 ttgtgcaaac aggtgagtat cggtaatttg taaaatcctg actctggcct caccagccag
     4201 aggaagggtt aacaggcttt ccaaatggtg tccttaggtt tcacgacgct aataaaccgg
     4261 aatcgccatc gctccatgtg ctaaacagta tcgcaaccag tccgctaaaa actgattaat
     4321 ttgatgcttt tcgtcgcgtt gatgcaactt tttattagga taatcatacc gcgctttgaa
     4381 gcgaaaaatc tgctggcttg aacacacttc agccaccatc gcgtcatgat acagacgcac
     4441 cgtcattgac ggaaggctcc agtaactgat cgcgggcgca gtctgttcta ttgtcaccag
     4501 ggtagtgtat cgggtcgatt ccacaatcgt cagccgatat tgtgcgtttg ccacctgata
     4561 gcttacagtt tcgccgggtg cgtcattgcg cggtaacaaa cggcgcaatt gtgaaaagtt
     4621 catctcgcac aggcgcatca tttcaggaaa gtcaggtgtg taacgcttca tttatgccca
     4681 ctcatttttt aacgcttgat gatgcagctg cagccattgc aaagcgatga ccgacgctgc
     4741 gttgtcgatt ttcccctctt ctacccactg gtatgcctgt tcccggctta ccacatgaac
     4801 gcgaatatct tcgttttcat cagccagacc atgaataccg cttgcggtcg tggcgtccac
     4861 ttcgcccacc ataattgacg aacgctcact ggtgcctccc gggcttgcca ggaaacttaa
     4921 caccggtttg gtccgtttga ctatcagtcc cgcctcttca atcgcttcgc gacgggcaac
     4981 atcttccaca ctttcaccct cttcaatcat cccggcaacc atctccagta gccagggggt
     5041 ttcactggtg tcgtatgcgg caatccgaat ctgctcaatc agcacaactt catcacgcac
     5101 tgggtcaaag ggtagcaaga ctgcggcgtg accgcgctca aaaatttccc gccgtacctc
     5161 atgactcatt tgcccgttga ataaacgatg acgaaatcta taaagatcta atgaaaaaaa
     5221 gccgcgataa agtgtttctc gtgcaataat ttctacatcg tttttgccaa gtgtaacggg
     5281 caggttgtct ggcttaagca ttgttaatgt cctggcacta atagtgaatt aaatgtgaat
     5341 ttcagcgacg tttgtctgcc gtttgagcag tcatgtgtta aattgaggca cattaacgcc
     5401 ctatggcacg taacgccaac cttttgcggt agcggcttct gctagaatcc gcaataattt
     5461 tacagtttga tcgcgctaaa tactgcttca ccacaaggaa tgcaaatgaa gaaattgctc
     5521 cccattctta tcggcctgag cctttctggg ttcagttcgt tgagccaggc cgagaacctg
     5581 atgcaagttt atcagcaagc acgccttagt aacccggaat tgcgtaagtc tgccgccgat
     5641 cgtgatgctg cctttgaaaa aattaatgaa gcgcgcagtc cattactgcc acagctaggt
     5701 ttaggtgcag attacaccta tagcaacggc taccgcgacg cgaacggcat caactctaac
     5761 gcgaccagtg cgtccttgca gttaactcaa tccatttttg atatgtcgaa atggcgtgcg
     5821 ttaacgctgc aggaaaaagc agcagggatt caggacgtca cgtatcagac cgatcagcaa
     5881 accttgatcc tcaacaccgc gaccgcttat ttcaacgtgt tgaatgctat tgacgttctt
     5941 tcctatacac aggcacaaaa agaagcgatc taccgtcaat tagatcaaac cacccaacgt
     6001 tttaacgtgg gcctagtagc gatcaccgac gtgcagaacg cccgcgcaca gtacgatacc
     6061 gtgctggcga acgaagtgac cgcacgtaat aaccttgata acgcggtaga gcagctgcgc
     6121 cagatcaccg gtaactacta tccggaactg gctgcgctga atgtcgaaaa ctttaaaacc
     6181 gacaaaccac agccggttaa cgcgctgctg aaagaagccg aaaaacgcaa cctgtcgctg
     6241 ttacaggcac gcttgagcca ggacctggcg cgcgagcaaa ttcgccaggc gcaggatggt
     6301 cacttaccga ctctggattt aacggcttct accgggattt ctgacacctc ttatagcggt
     6361 tcgaaaaccc gtggtgccgc tggtacccag tatgacgata gcaatatggg ccagaacaaa
     6421 gttggcctga gcttctcgct gccgatttat cagggcggaa tggttaactc gcaggtgaaa
     6481 caggcacagt acaactttgt cggtgccagc gagcaactgg aaagtgccca tcgtagcgtc
     6541 gtgcagaccg tgcgttcctc cttcaacaac attaatgcat ctatcagtag cattaacgcc
     6601 tacaaacaag ccgtagtttc cgctcaaagc tcattagacg cgatggaagc gggctactcg
     6661 gtcggtacgc gtaccattgt tgatgtgttg gatgcgacca ccacgttgta caacgccaag
     6721 caagagctgg cgaatgcgcg ttataactac ctgattaatc agctgaatat taagtcagcc
     6781 ctgggtacgt tgaacgagca ggatctgctg gcactgaaca atgcgctgag caaaccggtt
     6841 tccactaatc cggaaaacgt tgccccgcaa acgccggaac agaatgctat tgctgatggt
     6901 tatgcgcctg atagcccggc ccccgtcgtt cagcaaacat ccgcacgcac taccaccagt
     6961 aacggtcata accctttccg taactgatga cgacgacggg gcttcggccc cgtctgaacg
     7021 taaggcaacg taaagatacg ggttatctgc cgcattcttc ccccttctcg cttcaatttc
     7081 gaccagccat cctctattct gatgggtatt taccactggt cccggaagac aaaaatgaaa
     7141 cggacaaaat ccatacgcca cgcatcgttc cgcaaaaact ggagcgcacg ccatctgaca
     7201 ccagtcgctc tcgcggttgc cactgttttt atgctggctg gctgtgaaaa gagtgatgaa
     7261 acagtgtctc tctatcaaaa tgctgacgac tgttcagctg caaacccagg caaaagcgcc
     7321 gaatgtacca ccgcgtacaa caatgcgctg aaagaagccg aacgtactgc gccgaaatac
     7381 gccacccgtg aagactgtgt tgctgaattt ggtgaaggtc agtgccagca ggcaccagcc
     7441 caggctggca tggcaccaga aaaccaggcg caggcccagc aatccagcgg gagtttctgg
     7501 atgccgctga tggccggtta catgatgggg cgtctgatgg gcggcggcgc gggatttgca
     7561 cagcagccgc tgttctcctc gaaaaaccca gccagtccgg cttacggtaa atataccgac
     7621 gcgacgggta aaaactatgg cgcagcccag ccaggccgca ccatgaccgt accgaagacg
     7681 gcaatggcac caaaaccggc gaccaccact accgttaccc gtggcggttt tggtgaatct
     7741 gttgccaaac aaagcactat gcagcgtagt gcaaccggta cctcttctcg ttcaatgggt
     7801 ggctgatacc gatggaaaga gtcagtatta ccgagcgccc ggactggcgt gagaaagccc
     7861 acgaatacgg tttcaatttt cacaccatgt acggcgagcc gtactggtgt gaagatgctt
     7921 actacaagtt gaccctcgcc caggttgaaa agctggaaga agtcaccgcc gaactgcacc
     7981 agatgtgcct gaaagtggtg gaaaaagtga tcgccagcga tgagctgatg accaaattcc
     8041 gcattccaaa acacacctgg agttttgtgc gccagtcatg gctgacgcac cagccatcgc
     8101 tttattcgcg tcttgatctg gcgtgggatg gcactggtga acctaaactt ctggaaaata
     8161 acgccgatac gccaacgtca ctatacgagg cggcgttctt tcagtggatc tggctggaag
     8221 atcagcttaa cgccggtaac ttgccggagg gcagcgacca gtttaacagt ctgcaagaaa
     8281 aactgatcga tcgcttcgtt gagctgcgtg aacagtatgg cttccagttg ctgcatctca
     8341 cctgctgtcg cgacacggtg gaagatcgcg gaaccattca gtatttgcag gactgcgcaa
     8401 cggaagctga aattgctact gagttcctct acatcgatga tatcgggtta ggtgaaaaag
     8461 gtcagttcac ggatttacag gatcaggtaa tttccaacct gttcaagctg tatccgtggg
     8521 agtttatgct gcgtgagatg ttctccacca agctggagga tgcaggcgta cgctggctgg
     8581 aaccggcgtg gaagagcatt atctccaaca aggcacttct accgctgttg tgggagatgt
     8641 tcccgaatca cccgaacctg ctgcccgctt attttgcgga agatgatcat ccgcaaatgg
     8701 aaaaatatgt ggttaagccg atcttctccc gtgaaggcgc aaacgtgtcg atcattgaga
     8761 acggcaaaac cattgaagca gcggaaggtc cgtatggcga agaaggaatg attgttcagc
     8821 aattccaccc gttaccgaaa ttcggcgaca gctatatgct gattggtagc tggctggtga
     8881 acgatcaacc tgccggaatt ggcattcgtg aagaccgtgc attgatcacc caggatatgt
     8941 ctcggtttta tccacatatt tttgttgaat aatcaatgat accggatggc attcgccatc
     9001 cggtaattgt taacctacct gcaccgacag catactcagg ctgcccattt ctataccctc
     9061 aaccggaatg gtaattggct cctgcccatc ccacgcacct aacacataca acaacggcag
     9121 ataatgctct ggcgttgggt tcgataacgt gccaccttca tggtcgaggt aattcaccag
     9181 aggatgttgt tccactggcc cttgccacgt cagattcgct ttcacatact cattaaacga
     9241 cgtcgcccac ggatacggtg aactatcacc gtgccacttc actgtgcgca ggttatgcac
     9301 cacgttgcca ctggcgacca acattattcc ttcatctcgc agcgctgcca gtttgcgccc
     9361 catttcgaaa tgccaggcgg caggtttgct actgtcgata ctcaactgca ccatcgggat
     9421 atcggcgtca ggatacatct taatcagcac gccccaggaa ccgtggtcaa agccccaggc
     9481 ttctttatcc agcgtcaccg ggaccggcgc taacagctca accagatgct gtgccagcac
     9541 aggcgaaccc ggagcaggat aatgcgtatc gtacagcgcc tgcgggaagc caccaaagtc
     9601 atgaatcgtg ggcggcgtct ccatcgcggt cactcctgtt ccacgggtaa accagtgagc
     9661 cgaaaccacc acaatcgctt gcgggcgtgg caatgtcatc cccaacttct gccagctgcg
     9721 ggtatacaaa ttatcttcca gcacgttcat cggactaccg tgacctaaaa acaatgctgg
     9781 catacgtgtt gaagacatga tgatatcctt aactaaaggt gtcattttga tatcctcaca
     9841 atacgcttgt tcggcggagt aagaacccgg ataacaatga tgatgatcat cagttatttt
     9901 gacgatctgc ctgaaggtga agatttataa ggagttgtcg atgtcagtac ctctcattct
     9961 gaccatactg gcgggggcag ccacgtttat tggcgcgttt ctcggcgttc tcgggcaaaa
    10021 accctcgaac cgcttactgg cgttttcgct aggatttgcg gcggggatca tgttgctcat
    10081 ctcattaatg gaaatgcttc ctgccgcact agcagctgaa ggaatgtcgc ctgtgttggg
    10141 ttatggaatg tttatcttcg gtctgcttgg ctattttggc ctggaccgca tgttgccaca
    10201 tgcgcatccg caggatttaa tgcaaaaatc ggtgcagccg ttgccaaaat cgatcaagcg
    10261 cacagccatt ctgctcactc tcggcatcag tctgcataac ttcccggaag ggattgccac
    10321 ctttgtcacg gcgagcagca acctggagct gggatttggc atcgcactgg ccgtcgcgtt
    10381 gcacaatatt cctgaaggtc tggcggttgc aggcccggtt tatgcagcaa cgggttctaa
    10441 acgcacggca attctgtggg cggggatttc tggattggca gaaattcttg gtggtgtgct
    10501 ggcgtggtta atcctcggta gcatgatttc ccccgtggtc atggcggcaa tcatggcggc
    10561 ggttgcagga attatggtgg cgctctcggt tgatgaatta atgccgctcg ccaaagagat
    10621 tgaccctaat aataacccca gctatggcgt actatgtgga atgtcagtga tgggattcag
    10681 tttagtgctg ctacaaacgg cgggaattgg ttaaaagcaa catcagaagt tggccagcgc
    10741 atggacaact tctgctagct tcactaaagc cttattctac cttttcggct gtctcttctc
    10801 tcgtactgtt taagtatttg ttccgcgctt caagcagtgc gtctctgtcc tggcgaaacg
    10861 cccgacaata acgtttcaaa tgcaggaaat aacccaccac aacaagagca atcaatacaa
    10921 tccagtacca ggcgataaac atccttttac ccttaatata taacaatact aaacatttag
    10981 cgcataaatt tcacatatcc tttttcggat atattcatat ggtcgcataa taacggaaca
    11041 attatatgat tttgatcata atgaaaaaga atgcctgata ttaatgaaat tttctcatag
    11101 aaagagaagc ggaattaaca tcttgcatag cagggcaaaa taaaaaccga agccagctac
    11161 aacttcggtt tcttgattaa ggcagtaatt aagcagcggt tttcagctgg ctttacgctc
    11221 atgtgcctga cggtatgcca ccaggtcttc aatagtcacg agcgccatat tgtgtttatt
    11281 ggcaaactca atgcactctg gtgcacgcgc catcgtgcca tcgtcattag tcaactcaca
    11341 cagcacacca gccggtttaa agcctgccag cgtcatcaaa tcaatagtcg cttcagtatg
    11401 accgccacgc gtcagtacac cacctgcctg cgcgcgaagt gggaaaacgt ggcccgggcg
    11461 attcagatca gaaggttttg cgccatcggc aatcgctgcg cgaacggtcg taattcgatc
    11521 agcggcagaa acaccggtag tcacaccttc agccgcttca atggtcacgg taaaaccggt
    11581 gccataggcg ctggtgttat tttctaccat cattggcaga tcaagttgtt tacggcgatc
    11641 ttcagtgatg cacaggcaaa caataccgct accgtggcga atggtcagcg ccatctgctc
    11701 aacggtcatg gtttctgccg ggaagatcat atcgccttcg ttttcacggt cttcatcatc
    11761 aagcaccatt acaccgcgtc cttcacgcag cgcagccagt gcattttcaa cacgttcgaa
    11821 aggcgtacca aaagaggaaa gtagcgtctg attcatggta aaaaaacctc actaaaatta
    11881 tggttaccag aatcagggca gtcttaggag tggcggcata tagccaaaat aacgtgagcg
    11941 ggtccatgcc cgacagaatc gttactctct cccatccgga ctctaaccgt cggccccgga
    12001 attacaccgg atctgctgtc ctttgagttc gcacccaaag cgctcgcggg ctttcaactg
    12061 agttgattta ccgccggtgg ggaatttcgc cccgccctga gaataagcgg attcactata
    12121 acgctaatga ttagcggcag caacgcatag cttcacataa ttctggttta tgacttaccc
    12181 ttatcgcact acaatggcac tcaacaccta tcagtacagg gaaactacaa tgattgaccc
    12241 gaaaaaaatt gagcaaatcg ctcgccaggt tcacgaatca atgcctaaag gaatcaggga
    12301 gttcggggaa gatgtggaga aaaaaatccg ccaaacccta caagctcagc tgacgcgcct
    12361 cgaccttgta agccgtgaag agttcgacgt ccaaacgcaa gtcctgttac ggactcgtga
    12421 gaaactggcg ctgcttgaac aaagaatgag cgaactggaa aatcgcagta ctgagattaa
    12481 aaagcagcca gatccagaga ctctcccccc aacgctgtaa acatttgt
//
LOCUS       DBANUU010000126        12507 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000126 DBANUU010000000
VERSION     DBANUU010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.127"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(174..929)
                     /gene="mlaA"
                     /locus_tag="ACIE68_003655"
     CDS             complement(174..929)
                     /gene="mlaA"
                     /locus_tag="ACIE68_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFX5909912.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            1223..2155
                     /locus_tag="ACIE68_003656"
     CDS             1223..2155
                     /locus_tag="ACIE68_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFX5909913.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2231..2305
                     /locus_tag="ACIE68_003657"
     tRNA            2231..2305
                     /locus_tag="ACIE68_003657"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2264..2266,aa:Arg,seq:cct)
     gene            complement(2747..3994)
                     /locus_tag="ACIE68_003658"
     CDS             complement(2747..3994)
                     /locus_tag="ACIE68_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFX5909914.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTIYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(4066..4980)
                     /locus_tag="ACIE68_003659"
     CDS             complement(4066..4980)
                     /locus_tag="ACIE68_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFX5909915.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            5196..6629
                     /gene="cscA"
                     /locus_tag="ACIE68_003660"
     CDS             5196..6629
                     /gene="cscA"
                     /locus_tag="ACIE68_003660"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFX5909916.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(6637..7632)
                     /locus_tag="ACIE68_003661"
     CDS             complement(6637..7632)
                     /locus_tag="ACIE68_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFX5909917.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            7875..8093
                     /locus_tag="ACIE68_003662"
                     /pseudo
     CDS             7875..8093
                     /locus_tag="ACIE68_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            8111..9439
                     /gene="dsdA"
                     /locus_tag="ACIE68_003663"
     CDS             8111..9439
                     /gene="dsdA"
                     /locus_tag="ACIE68_003663"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFX5909918.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(9547..11085)
                     /gene="emrY"
                     /locus_tag="ACIE68_003664"
     CDS             complement(9547..11085)
                     /gene="emrY"
                     /locus_tag="ACIE68_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFX5909919.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGIG"
     gene            complement(11085..12248)
                     /gene="emrK"
                     /locus_tag="ACIE68_003665"
     CDS             complement(11085..12248)
                     /gene="emrK"
                     /locus_tag="ACIE68_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFX5909920.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQF"
BASE COUNT         3522 a         3025 c         2829 g         3131 t
ORIGIN      
        1 ttccagtttt ttgatttttc aactcctgaa tatcggaaat agtcagtgac gaacatcagt
       61 atttttacat cattacgtcc gggtcattgt cggtttatct ccttttactt gtggtaattg
      121 agggataaca acaaaaaagg tgagtattgc gactcacctt ttttatttgt ttcttattca
      181 gaatcgatat cttttaaatc atcctgaatc gcttgcgcgt tcggattttc ctgcggtttg
      241 agttcgccgc cattagcgat gaaatcatga cgctggaagt acgcttcgcg caccataata
      301 taaggatcgg acgactgacg cagcagacca tcggaatcca acaactgcgc acgagtttct
      361 atcccttcaa gcgtccattt acccacagac atcggccagg tcagccagga aagaaccggg
      421 taaagaccat ccgccatatc accaccgtca tcacgcagcg tgaagctacc gtagaacggt
      481 aactggacgt aaggcccata acccacgcca taatgaccaa gcgtactacc gaagcggtga
      541 ggttcagtcc gttgcagttt cgggttcgcc atccctgcaa catcaataaa accgcccatc
      601 cccaaaatgg tgttcaggaa aaagcgggta aagtggacca tcccctgata agggtcgccc
      661 tgcaagaagt agttaaccat caccgcaggt tcttcaaggt tgccagtaaa gttgctcaaa
      721 ccgttacgcg ccggttgcgg aacataatca cgccaggcga cagcgaccgg tcgaacaata
      781 tacgggtcta atacattgaa gttgaagttg tacatggtgc ggttgaaccc ttctaacggg
      841 tcagaacgcc cttgctgatc tgtaccggaa ctcgcacacc ccaccagaag cgtagttccc
      901 agagcaagcg ccgacaggcg aagcttcata aatgtctccc tgttttttta tggcttatgc
      961 agtttgccat ccatgacgga acgataccgt atccgcctgt ttaggtgtgg gcgattgtaa
     1021 cagcacgtca actgatgtcc agacgccctg atttgctgat ttgatcatag cctggtaatc
     1081 gccgccctgt aggctacttg attctataga aacagaaaaa ggcaaacgca gccttttcta
     1141 cattttcaga gtaactcccg ccagttgcga gcaaaaaagc cgctacgctt tagctatacg
     1201 tgctaattca agagaagaga ccatggacaa cgacaaaatt gatcaacaca gcgacgaaat
     1261 tgaagttgag agcgaagaaa aagagcgcgg caaaaaaata gaaatagatg aagaccgact
     1321 cccctcccgg gcgatggcaa ttcatgagca tatccgccag gatggtgaaa aagagctgga
     1381 acgcgacgca atggcgctac tgtggtcagc cattgcggcg ggtctgtcga tgggcgcttc
     1441 gctactggca aaagggatat ttcatgtcga actggaagga gtgccaggca gcttcttact
     1501 ggagaatctc ggttatacct ttggttttat tatcgtcatt atggcccgcc agcaattatt
     1561 taccgaaaac accgtgactg cggtactacc cgtcatgcaa aaaccgacaa tgagcaacgt
     1621 cggcttactt atgcgattat ggggcgtcgt gctgctgggt aatattctcg ggacaggtat
     1681 tgctgcatgg gcatttgaat atatgcctat cttcaatgaa gaaactcgcg atgcatttgt
     1741 caaaatcggc atggatgtga tgaagaacac ccccagcgag atgtttgcca acgcgatcat
     1801 ttccggctgg ctgatcgcca ctatggtttg gatgtttcct gctgcgggtg cggcaaagat
     1861 tgtggtgatt atattgatga cctggcttat tgcactgggt gacaccaccc acatcgttgt
     1921 cggttctgtt gaaatcctct atctggtgtt taacggcacg ctgcactgga gcgatttcat
     1981 ctggcccttc gcactaccta ctttagcggg gaacatctgc ggcggcacct ttatcttcgc
     2041 gttaatgagt catgcacaga ttcgtaacga catgagcaac aagcgtaaag cagaagcacg
     2101 ccaaaaagca gaacgtgcgg aaaacattaa gaaaaatgat aaaaacccag cataaatggc
     2161 gagggtttaa gcaatcgagc ggcagcgtac ttaccccgca ctccattagc gggtatactc
     2221 atgccgcatt gtcctcttag ttaaatggat ataacgagcc cctcctaagg gctaattgca
     2281 ggttcgattc ctgcagggga caccagatac ccttcaaacg aaatctacct tcaccccgta
     2341 aaagatgggt ttggcagcac acttgcctta tatctactca tttttactgc aacaggttga
     2401 aatctcagca ctgtcagaaa gcgctgatga ctaaacagcc ctgggccggg cgatgtaacc
     2461 atcacacaga atcctgatag cgaaatatgg cgtgactcga tacttcactc cgcaatgcat
     2521 tccttgatga attcgcagga ccgtgataca cgggacaggt cactgaatga cgacaatgtc
     2581 ctggaaatca gcgaaccgcg catctgaagt acatttgaac gactgtacca gaacatgaat
     2641 gaggcgtttg gattaggcga ttattagcag ggctaagcat tttactatta ttattttccg
     2701 gttgagggat atagagctat cgacaacaac cggaaaaagt ttacgtctat attgctgaag
     2761 gtacaggcgt ttccataact atttgctcgc gttttttact caagaagaaa atgccaaata
     2821 gcaacatcag gcagacaata cccgaaattg cgaagaaaac tgtctggtag cctgcgtggt
     2881 caaagagtat cccagtcggc gttgaaagca gcacaatccc aagcgaactg gcaatttgaa
     2941 aaccaatcag aaagatcgtc gacgacaggc gcttatcaaa atttgccacg ctgtatttga
     3001 agacggatat gacacaaagt ggaacctcaa tagcatgtaa cagcttcact aatgaaataa
     3061 tccaggggtt aacgaacagc gcgcaggaaa ggatacgcaa cgccataatc acaacaccga
     3121 taagtaatgc attttttggc cctacccgat tcacaaagaa aggaataatc gccatgcata
     3181 gcgcttcgag taccacctgg aatgagttga gataaccata caggcgcgtt cctacatcgt
     3241 gtgattcgaa taaacctgca taaaagacag gaaaaagttg ttgatcaaaa atgttataga
     3301 aagaccacgt ccccacaata aatatgacga aaacccagaa gtttcgatcc ttgaaaactg
     3361 cgataaaatc ctcttttttt acccctcccg catccgccgc tacgcactgg tgatccttat
     3421 ctttaaaaca catgttgatc atcataaata cagcgccaaa tagcgagacc aaccagaagt
     3481 tgatatgggg actgatacta aaaaatatgc cggcaaagaa cgcgccaata gcatagccaa
     3541 aagatcccca ggcgcgcgct gttccatatt cgaaatgaaa atttcgcgcc attttttcgg
     3601 tgaagctgtc aagcaaaccg catcccgcca gataccccag gccaaaaaag agcgccccca
     3661 gaattagacc tacagaaaaa ttgctttgca gtaacggttc ataaacgtaa atcataaacg
     3721 gtccggtcaa gaccaggatg aaactcatac accagatgag cggtttcttc agaccgagtt
     3781 tatcctgaac gatgccgtag aacatcataa atagaatgct ggtaaactgg ttgaccgaat
     3841 aaattgtacc taattccgtc cctgtcaacc ctagatgtcc tttcagccaa atagcgtata
     3901 acgaccacca cagcgaccag gaaataaaaa agagaaatga gtaactggat gcaaaacgat
     3961 agtacgcatt tctgaatgga atattcagtg ccataattac ctacctgtcg ttaaaaaatt
     4021 cacgtcctat ttagagataa gagcgacttc gccgtttact tctcactatt ccagttcttg
     4081 tcgacatggc agcgctgtca ttgccccttt cgccgttact gcaagcgctc cgcaacgttg
     4141 agcgagatcg ataattcgtc gcatttctct ctcatctgta gataatcccg tagaggacag
     4201 acctgtgagt aacccggcaa cgaacgcatc tcccgccccc gtgctatcga cacaattcac
     4261 agacattcca gcaaaatggt gaacttgtcc tcgataacag accaccaccc cttctgcacc
     4321 tttagtcacc aacagcatgg cgatctcata ctcttttgcc agggcgcata tatcctgatc
     4381 gttctgtgtt tttccactga taagtcgcca ttcttcttcc gagagcttga cgacatccgc
     4441 cagttgtagc gcctgccgca aacacaagcg gagcaaatgc tcgtcttgcc atagatcttc
     4501 acgaatatta ggatcgaagc tgacaaaacc tccggcatgc cggatcgccg tcatcgcagt
     4561 aaatgcgctg gtacgcgaag gctcggcaga caacgcaatt gaacagagat gtaaccattc
     4621 gccatgtcgc cagcagggca agtctgtcgt ctctaaaaaa agatcggcac tggggcggac
     4681 cataaacgta aatgaacgtt ccccttgatc gttcagatcg acaagcaccg tggatgtccg
     4741 gtgccattca tcttgcttca gatacgtgat atcgactccc tcagttagca gcgttctttg
     4801 cattaacgcc ccaaaaggat catcaccgac ccgacctata aatccactta ttccgcctaa
     4861 tctggcgatt cccaccgcaa cgttagctgg cgcgccgcca ggacaaggca gtaggcgccc
     4921 gtctgattct ggcaagagat ctacgaccgc atcccctaaa acccatactt tggctgacat
     4981 ttttttccct taaattcatc tgagttacgc atagtgataa acctctttct cgcaaaatcg
     5041 tcatggattt actaaaacat gcatattcga tcacaaaacg tcatagttaa cgttaacatt
     5101 tgtgatattc atcgcattta tgaaagtaag ggactatatt tttataaaag ttaacgttaa
     5161 caattcacca aatttgctta accaggatga ttaaaatgac gcaatctcga ttgcatgcgg
     5221 cgcaaaacgc actagcaaaa cttcatgagc accggggtaa cactttctat ccccattttc
     5281 acctcgcgcc acctgccgga tggatgaacg atccaaacgg cctgatctgg tttaacgatc
     5341 gttatcacgc gttttatcaa catcacccga tgagcgaaca ctggggacca atgcactggg
     5401 gacatgccac cagcgacgat atgatccact ggcagcatga gcctattgcg ctagcgccag
     5461 gagacgataa tgacaaagac gggtgttttt caggtagtgc tgtcgatgac aatggtgtcc
     5521 tctcacttat ctacaccgga cacgtctggc tcgatggtgc aggtaatgac gatgcaattc
     5581 gcgaagtaca atgtctggct accagtcggg atggtattca tttcgagaaa cagggtgtga
     5641 tcctcactcc accagaagga atcatgcact tccgcgatcc taaagtgtgg cgtgaagccg
     5701 acacatggtg gatggtagtc ggggcgaaag atccaggcaa cacggggcag atcctgcttt
     5761 atcgcggcag ttcgttgcgt gaatggacct tcgatcgcgt actggcccac gctgatgcgg
     5821 gtgaaagcta tatgtgggaa tgtccggact ttttcagcct tggcgatcag cattatctga
     5881 tgttttcccc gcagggaatg aatgccgagg gatacagtta ccgaaatcgc tttcaaagtg
     5941 gcgtaatacc cggaatgtgg tcgccaggac gactttttgc acaatccggg cattttactg
     6001 aacttgataa cgggcatgac ttttatgcac cacaaagctt tttagcgaag gatggtcggc
     6061 gtattgttat cggctggatg gatatgtggg aatcgccaat gccctcaaaa cgtgaaggat
     6121 gggcaggctg catgacgctg gcgcgcgagc tatcagagag caatggcaaa cttctacaac
     6181 gcccggtaca cgaagctgag tcgttacgcc agcagcatca atctgtctct ccccgcacaa
     6241 tcagcaataa atatgttttg caggaaaacg cgcaagcagt tgagattcag ttgcagtggg
     6301 cgctgaagaa cagtgatgcc gaacattacg gattacagct cggcactgga atgcggctgt
     6361 atattgataa ccaatctgag cgacttgttt tgtggcggta ttacccacac gagaatttag
     6421 acggctaccg tagtattccc ctcccgcagc gtgacacgct cgccctaagg atatttatcg
     6481 atacatcatc cgtggaagta tttattaacg acggggaagc ggtgatgagt agtcgaatct
     6541 atccgcagcc agaagaacgg gaactgtcgc tttatgcctc ccacggagtg gctgtgctgc
     6601 aacatggagc actctggcta ctgggttaac ataatatcag gtggaacaac ggatcaacag
     6661 cgggcaaggg atccgcgtca ctcttccccc ttcacgacct tcaataatat gcaatgcagc
     6721 ttcccgcccg ataatgtcat gtggaagctg aattgtggtc agcggcggta aaaacagatg
     6781 cccgacgcca accagattat caaagcccat tacggcgaca tcctgcggga ttcgtacccc
     6841 cttcgccaga agaacctgat aagccacaaa ggctgcgcga tcgttaccac atatcagaac
     6901 atcaaaatct ggtttgcccg atttgaagtg gtcattgagt aaacttgcga gatcggtgta
     6961 gtgatcatca cctgttgcca tgtgaaattg tttcacctca gccagatctc gtccagcatc
     7021 acgccaggcc tgctcaaatc cctgccgacg ataccctgtt gccaacgcac tttccggtag
     7081 ccagaagcat aacggttgac gatagcccgc cgcgagcaaa tgctgtgttg attcatattg
     7141 tgcagtgtaa tcatcaggga tataactggg taacgctggg tcatccgcca cacagttcgc
     7201 caatacaata ttttcaccat acagagactc aggcagcgtg atatgtcgca gccccattgt
     7261 agtatagata atgccatccg gacggtgggc aagcagctga cgtgccgcgc gggcagcgtc
     7321 atcttcagaa aaaatattga ttaaaaaact attccagccg aactcgctgg cggtttgctc
     7381 aatggcaagc agaatatcaa cagagaaagg agtggtagcc gtgtcctgcg ccagcacggc
     7441 gagagtcgac ggcttacgtc cttgagcgcg catcttacgg gcggaaagat caggaacata
     7501 attcagggtc tggattgcct gcaatacgcg gtcacgcgtt gcaggacgca cagattctgc
     7561 attatgcatc acccgggaga ctgtcatcat cgacactccc gccaggcgtg cgacatcctt
     7621 taatgaagcc atacccaagc cgtttgccgt aaaacgggca ctgtagcaga aacagacgtc
     7681 actggcgaga tccaacgata ttctcaacgc cctatcaccc gacacggcaa tacaataaaa
     7741 aataacaata attgccggat aattgtactc aattccgcct ctgttctcgc caacgagtaa
     7801 ccatataggc aagtaagtaa aacatgccaa ctggcatcgc caacctggag aagaaaatat
     7861 aaatatggat cattgtgctg ccgctgtatc ccgacatcag cccggaaatt attgcgattg
     7921 ctatcggttc aggtgcaatt ggctgcacga tcgttacgga ctcgcttttc tggctagtga
     7981 agcaatattg cggcgctacg ctcaatgaaa catttaaata ctatacgaca gcgacattta
     8041 tcgcttcagt cgtcgctctg gcgggcacat tcctgctgtc atttatcatc taagcgcaaa
     8101 gagacgtact atggaaaacg ctaaaatgaa ctcgctcatc gcccagtatc cgttggtaaa
     8161 ggatctggtt gctcttaaag aaaccacctg gtttaatcct ggcacgacct cattggctga
     8221 aggtttacct tatgttggcc tgaccgaaca ggatgttcag gacgcccatg cgcgcttatc
     8281 ccgttttgca ccctatctgg caaaagcatt tcctgaaact gctgccactg gggggattat
     8341 tgaatcagaa ctggttgcca ttccagctat gcaaaaacgg ctggaaaaag aatatcagca
     8401 accgatcagc gggcaactgt tactgaaaaa agatagccat ttgcccattt ccggctccat
     8461 aaaagcacgc ggcgggattt atgaagtcct ggcacacgca gaaaaactgg ctctggaagc
     8521 ggggttgctg acgcttgatg atgactacag caaactgctt tctccggagt ttaaacagtt
     8581 ctttagccaa tacagcattg ctgtgggctc aaccggaaat ctggggttat caatcggcat
     8641 tatgagcgcc cgcattggct ttaaggtgac agttcatatg tctgctgatg cccgggcatg
     8701 gaaaaaagcg aaactgcgca gccatggcgt tacggtcgtg gaatatgagc aagattatgg
     8761 tgttgccgtc gaggaaggac gtaaagcagc gcagtctgac ccgaactgtt tctttattga
     8821 tgacgaaaat tcccgcacgt tgttccttgg gtattccgtc gctggccagc gtcttaaagc
     8881 gcaatttgcc cagcaaggcc gtatcgtcga tgctgataac cctctgtttg tctatctgcc
     8941 gtgtggtgtt ggcggtggtc ctggtggcgt cgcattcggg cttaaactgg cgtttggcga
     9001 tcatgttcac tgcttttttg ccgaaccaac gcactcccct tgtatgttgt taggcgtcca
     9061 tacaggatta cacgatcaga tttctgttca ggatattggt atcgacaacc ttaccgcagc
     9121 ggatggcctt gcagttggtc gcgcatcagg ctttgtcggg cgggcaatgg agcgtctgct
     9181 ggatggcttc tataccctta gcgatcaaac catgtatgac atgcttggct ggctggcgca
     9241 ggaagaaggt attcgtcttg aaccttcggc actggcgggt atggccggac ctcagcgcgt
     9301 gtgtgcatca gtaagttacc aacagatgca cggtttcagc gcagaacaac tgcgtaatac
     9361 cactcatctg gtgtgggcga cgggaggtgg aatggtgccg gaagaagaga tgaatcaata
     9421 tctggcaaaa ggccgttaat aacgtttcca cgcagcatcg caatcctttc cctgggtgag
     9481 cgatgctgcc gatggcgcag acttaagatc cccggtctta cccgctataa ccccctcctt
     9541 ttatcatcac ccaatgcctt tcgctgtaaa cggcggtttc gcaaaccaaa ccaaaaccgt
     9601 aagtaagata aaagcaatag ccgccatacg gaaaatctca tttgcagaaa tagaaagtga
     9661 ctgctgggtt atttcattat taatttcatt aagaactcct gaaagcgaac catagtattt
     9721 atccataatt tgtgacgatg aattaaacac ggggttaaat tgatcgatgg ttgctgtcaa
     9781 ctgactatgg tgtaacgatt cgcgtcgtcc ccacagcgtc attgtcaacg acgtaccaac
     9841 tgatcctgac aaggtacgaa aaaaattact catactcgag gcattggcaa atttattatc
     9901 tggcaagcct gaaaacgaaa tcgttgttaa gggtaaaaag aaacaggcaa cggcgaatcc
     9961 ctgaaaaaac tgcggcaaaa tgatgcctgt aaaatcaatc gttggcataa atgtcacaga
    10021 acgccagtaa tagcaaaccg catacatcaa aaaactaaat gtcactaaca accgcatgtc
    10081 tattttgttg ccataacgtc ctatcaaagg tgaaattaat agtggcatga tgccgatggg
    10141 cgcataagca agtccggccc atatcgcatt ataccccatc gtttcctgga gtaactgcgg
    10201 cataaggacg atcgctccag agtaaaataa atacgcgcat gtgatactca caataccaat
    10261 ggtgaagtta cgggacttaa acaaactgag atcaagaatc gggttctctg aggtcgactc
    10321 ccaaatgact aaagagatca gagaaataac tgatactact gttaatatta ttattgtact
    10381 cgagttgaac caatccagat cgcgcccttt atcaagcata atttgcaagc caccaacacc
    10441 gagcactaac agggtcagtc ctggtagatt cattttgacc ggtgaagtct cagtttctct
    10501 tcctttaagt aaggttaagc ataatgtcag gacgataatc cccataggga cattgattaa
    10561 aaatatccaa ccccagctaa agttatcaca aatataaccg cccaatatcg gcccacatat
    10621 cggagcgata atcacggtca ttgaccataa tgccagagca aatgttcttt tttctggcgg
    10681 ataattcctt aataataaac tctgtgacag tggaattaac ggccccgcca ttaacccctg
    10741 aacgactcta aaaaatatca gcacatcaag attggtcgat aggctacaca ttaatgaaga
    10801 cagcgaaaaa aaggttactg aaagtaaaaa taatcttaat tcgcctattc tttgtgccaa
    10861 cctgccagta acagggatcg caatggcatt tgctacacca aacgaggtga taacccaggt
    10921 gccttcgtct gttgatgctc ccagaaagcc agatattgtc ggtattgcga cgttagaaat
    10981 agtggaatcc aacatttgca taaatgtcgc taatgacaat gcaatagtga cgcaccataa
    11041 cgtcccaccg gttaatggtg ccggagttga tttagtgatt gccattaaaa ttgtccatta
    11101 tgcgaaataa tgttgctaat ttctttttct atcggactgg tatcgataac taaagcctta
    11161 ctggtataag ccggcatgga ggtcacggtt gaagccagct caggcatctc ggcaatgtct
    11221 tcgttcttcg tatcaatagt tgctgtcatc gataaaccaa tacgcaaggg atgttccatg
    11281 agttcttttg gatcaagaga aacttcaacc ggtacacgct gaacgatttt gatccagttc
    11341 cctgtcgcat tttgtgcagg taataaggag aacgcattgc cggttcccat attgatccct
    11401 gtcacccgac catgaaacac aacattttca ccataaagat cgctgataat attgaccgat
    11461 tgaccaatcc gtacatccgt gagttgtgtt tctttaaagt tggcattaac ccacatttga
    11521 cgtgccggta cgacagccat taacgattgt ccggggctca ctgtttcgcc gacctgaaca
    11581 cttcgctggg caatatagcc ggtaaccgga ctcttaatat ccgtacgttt aagcgccaac
    11641 caggcttctt tagttgcatc cgccgcttca acgacttgtg gctgacggtt taatggtgtg
    11701 ttcattacta aagctttatt cgctttataa gcctggatag cggcattcaa tgccgctttg
    11761 ctacttatta acgtatcttt ggtatgctcc agcgtttctt ttgaaataac cccctgcttc
    11821 gctaacggca ctcgacggtt ataatcttct aaagattgtt gatactgaat acgtgctgaa
    11881 gcgacttcgg cactgtattg tttatcctgt aagtatagtt tattcgtttg ccgaacaata
    11941 tttgccagat tatttttagc tttattgagt gcgatagtgg catcagtttt atccagtgaa
    12001 actaaaatgt caccttgtcg aacgtagttc gtatctttat gattaacgac agtgacacta
    12061 cctgagactt gtgcagaaat tggatctgca ttccccgtga cataggcgtc atctgtacta
    12121 atcatgtctt ctaattccat tgaccaatag gcataggcac ctgaaaacgc aataaataaa
    12181 actaccgcca ataaagaaaa gtattttctt ctgttagaat gttttttatt tgaattaatc
    12241 tgttccacta ttatctctca tttctcatag atgaaattta tgaattgaat gaaaagagca
    12301 ttcattcttc atatcaaaca cagtgcatgt gttgcacatt atcccaggaa cgttggaggc
    12361 ctaatttaaa tgaggtgcaa aaaaaacaac agaggactaa accgtggctt ttgcaataca
    12421 attcttacgc ctgtaggatt agtaagaaga cttatagtgc taacttgaaa ctataaatca
    12481 ccggtacaat ccctgatttt attgttg
//
LOCUS       DBANUU010000127        12399 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000127 DBANUU010000000
VERSION     DBANUU010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.128"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(58..339)
                     /locus_tag="ACIE68_003666"
     CDS             complement(58..339)
                     /locus_tag="ACIE68_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFX5909921.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(602..1711)
                     /gene="apbC"
                     /locus_tag="ACIE68_003667"
     CDS             complement(602..1711)
                     /gene="apbC"
                     /locus_tag="ACIE68_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFX5909922.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1843..3876
                     /gene="metG"
                     /locus_tag="ACIE68_003668"
     CDS             1843..3876
                     /gene="metG"
                     /locus_tag="ACIE68_003668"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFX5909923.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVETSKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4017..7811
                     /locus_tag="ACIE68_003669"
     CDS             4017..7811
                     /locus_tag="ACIE68_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFX5909924.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKVNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIKWDYANNVIIILPSGIAPDYLEQYSRFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKAIAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPRLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDVCSADSLAGFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDESRSNDAVNRYKLLKKDARTVAAQQVAR
                     LESAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYST
                     ADDDLFTLPEGDISIGTPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWIKGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            7821..11453
                     /gene="yehI"
                     /locus_tag="ACIE68_003670"
     CDS             7821..11453
                     /gene="yehI"
                     /locus_tag="ACIE68_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFX5909925.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRKWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQDEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVGL
                     ECPRTHFHSKEWLKVVANDPTAVRKLEHYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAIPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYHKNFP
                     HATLAALAELLALTEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAEE
                     MMQWLSTQARAVLNSYLSAPPKPVIDSTDNSNLPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGERERLAATEPARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLM
                     LAFSHRPKETFPLILNFGATELALPIARVWHRFAGQRNLARQWILQWPEHTATALIPL
                     IFTKSSDKSEAALLALRLLYEHGHGELLQTVANRWQRTDLWPALEQLLKQSPIEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPVKDNWAFLALSLFGDESTARDLTTQILGWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLETALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     AYPANEPRCLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTVEMRSEFAQLF
                     ADYEIMPPFRQLARCTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11514..11831
                     /gene="yehK"
                     /locus_tag="ACIE68_003671"
     CDS             11514..11831
                     /gene="yehK"
                     /locus_tag="ACIE68_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFX5909926.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLHPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
BASE COUNT         3253 a         3152 c         3029 g         2965 t
ORIGIN      
        1 aatcatttat ataaatgatt tcggctttct aaatgcaatt aaaatcaggt tgccaaatta
       61 atcgtagcga tttaacacga ttcgacgttt gctatcgcta cctgcaacaa ccacttctgt
      121 cgttacacct ttgacgggcg aagaaaacag atgacgggtg tcaacattat acgttaactg
      181 accacaatgc tgttgaggtt gaccattaac catagccata ctgcatgttg ggggactgat
      241 cctgccatta atcgctaatg tggcagtctc tgaagagaat gcgggagaag ccaatccata
      301 ggcaagaaaa ataattcctg acagccaata cttattcatt gaacgttatc cctgtagtaa
      361 aggttatgcc tggatagaat gagtgcataa caaactatag ctgtacatcc actacacagc
      421 cacgaaggat gataatgaag cattgcctgt atgatcaatc gactttgtag agtttcggac
      481 gaaggtccgc agaatattcg cagtattaaa taagtgttca ttaatgcggc aaaaactaat
      541 acaccgcatc aatgtaacat ctctgcggtg tatgaagaat aaccagaatg gttaataggc
      601 gttagaccgc gcggaaggaa atctcgcctg gaatgacttc accctgccag tagagctgag
      661 ctgcaacgcg gtcagccagc tggcggtaga tagcggtaaa ttcgctctcc ggacggctga
      721 taaccgttgg cgtgccttta tcgagatcct cacgtaagga gatgtgtaac ggcatctgcc
      781 ccagcagctg tgtgtggtat ttttcagcca gtttctcagc accaccggta ccgaaaatcg
      841 gttcatggtg accacagtta ctgcaaatat gcacactcat gttctcgaca ataccaagca
      901 ccggaacctc gactttctcg aacatcacaa tgcctttttt cgcatcgatc aacgcgatgt
      961 cctgcggggt ggtgaccacg actgcaccag ttactggaat gttttgcgcc agcgtcaatt
     1021 gaatgtcacc ggtgcctggc ggcatatcaa gcaccagata gtcgagatcc ggccacaagg
     1081 tttcctgcaa catctgcatt aacgccttgc tcgccatcgg tccacgccac accatcgcat
     1141 tgtcgtcagt gaccagataa ccaattgaat tggttgccag gccatgagac ataatcggtg
     1201 ccatgtgggt accgtccggc gacgttggac gctgattttc tgcgcccagc atggtgggaa
     1261 ttgatgggcc gtagatatcg gcgtccagaa tgccgacttt cgccccttcc gccgccagtg
     1321 ccagcgccag gtttaccgcc gtggaggatt tacccacgcc gcctttaccg gagctgaccg
     1381 caatgatgtt cttcacacca ttaatacccg gctgattctt cacgcgtttt aaggtggcga
     1441 tattgtgcga cagtttccag tcgatagctt tcgccccggt gatacgcagc agttcggcgc
     1501 tacattgctc ttttaactct tcaaacgcgc tatgccagac gaatggcata accagttcca
     1561 catgcaacgt gtcgtccatc caggcgacat ggtgcaacgc tttcagcgtg gtgaggttat
     1621 gcttcagggt tgggtgctga aaattggcca gtgtcccggc gaccattgcg cgcaaggctt
     1681 ctggtgattt ggcctgggat tgttcgttca tcccgactcc ttttttgtat agataaacca
     1741 tcagctgata gtttacctga agaatataga gaagtactta cttaacattt tcccatttgg
     1801 tactatctaa ccccttttca ctattaagaa gtaatgccta ctatgactca agtcgcgaag
     1861 aaaattctgg tgacgtgcgc actgccgtac gctaacggct caatccacct cggccatatg
     1921 ctggagcaca tccaggctga tgtctgggtc cgttaccagc gaatgcgcgg ccacgaggtc
     1981 aacttcatct gcgccgacga tgcccacggt acaccgatca tgctgaaagc tcagcagctt
     2041 ggtatcaccc cggagcagat gattggcgaa atgagtcagg agcatcagac tgatttcgca
     2101 ggctttaaca tcagctatga caactatcac tcgacgcaca gcgaagagaa ccgccagttg
     2161 tcagaactta tctactctcg cctgaaagag aacggtttta ttaaaaaccg caccatctct
     2221 cagctgtacg atccggaaaa aggcatgttc ctgccggacc gttttgtgaa aggcacctgc
     2281 ccgaaatgta aatccccgga tcaatacggc gataactgcg aagtctgcgg cgcgacctac
     2341 agcccgactg aattgatcga gccgaaatcg gtggtttctg gcgctacgcc ggtgatgcgt
     2401 gattctgaac acttcttctt tgatctgccc tctttcagcg aaatgttgca ggcatggacc
     2461 cgcagcggtg cgttgcagga gcaggtggca aataaaatgc aggagtggtt tgaatccggc
     2521 ctgcaacagt gggatatctc ccgcgacgcg ccatactttg gttttgaaat tccgaacgcg
     2581 ccgggcaaat atttctacgt ctggctggac gcaccgattg gctacatggg ttctttcaag
     2641 aatctgtgcg acaagcgcgg cgacagcgta agcttcgatg aatactggaa gaaagactcc
     2701 accgccgagc tgtaccactt catcggtaaa gatattgttt acttccacag cctgttctgg
     2761 cctgccatgc tggaaggcag caacttccgc aagccgacca acctgtttgt tcatggctat
     2821 gtgacggtga acggcgcaaa gatgtccaag tctcgcggca cctttattaa agccagcacc
     2881 tggctgaatc attttgacgc tgacagcctg cgttactact acactgcgaa actctcttcg
     2941 cgcattgatg atatcgatct caacctggag gatttcgttc agcgtgtgaa tgccgatatc
     3001 gtgaacaaag tggttaacct ggcctctcgt aatgcgggct ttatcaacaa gcgttttgac
     3061 ggcgtgctgg caagcgaatt ggctgacccg cagttgtaca aaaccttcac tgatgccgct
     3121 gaagtgattg gtgaagcgtg ggaaagccgc gaatttggta aagcagtgcg cgaaatcatg
     3181 gcgctggctg atctggctaa ccgctatgtc gacgagcagg ctccgtgggt ggtggcaaaa
     3241 caggaaggcc gcgatgccga cctgcaggca atttgctcaa tgggcataaa cctgttccgc
     3301 gtgctgatga cttacctgaa gccggtactg ccgaaactga ccgagcgcgc agaagcattc
     3361 ctcaatacag aactgacctg ggatggtatc cagcaaccgc tgctgggcca caaagtgaat
     3421 ccgttcaaag cgctgtataa ccgcatcgat atgaagcagg ttgaagcact ggtggaaacg
     3481 tctaaagaag aagtgaaagc agctgccgcg ccagttactg gcccactggc agatgatccg
     3541 attcaggaaa ccatcacctt tgacgacttc gcaaaagttg acctgcgcgt tgcgctgatc
     3601 gaaaacgcag agtttgttga aggttctgac aaactgctgc gcctgacact ggatctcggc
     3661 ggtgaaaaac gcaatgtctt ctccggtatt cgttctgcct acccggatcc gcaggcactg
     3721 atcgggcgtc acaccattat ggtggccaac ctggcaccgc gtaaaatgcg ctttggtatc
     3781 tccgaaggca tggtaatggc tgctggtcct ggcgggaaag atatcttcct gttaagcccg
     3841 gatgctggtg ctaaaccagg ccatcaggtg aaataatccc cctttaaggc gctgcatcga
     3901 cagcgccttt tctttataaa ttcctaaagt tgttttcttg cgattttgtc tctctctaac
     3961 ccgcataaat actggtagca tctgcattca actggataaa atcacaggga tgcagaatga
     4021 gacactttat ctatcaggac gaaaaatcac ataaattctg ggcagttgag caacagggaa
     4081 acgagttgca tatcagttgg ggaaaagttg gcaccaaagg gcaaagccag ataaaaagtt
     4141 tttcagatgc tgcggcagcg gcaaaagcgg agcttaagct gattgcggag aaggtgaaga
     4201 aggggtatgt ggagcaagcg aaggttaatt ctttgcaacc ttcccaaacg gtaacgggct
     4261 ctctcaaggt agcggactta tccaccatta ttcaggaaca accctctttc gtagcagaaa
     4321 cccgtgcgcc tgacaaaaat acagatgctg ttttaccgtg gctggcgaaa gatattgctg
     4381 tcgtttttcc gcccgaagtt gtacacacca cgttaagtca tcgccgcttt cccggagttc
     4441 ctgttcagca agcagacaaa ttgacccaat tacgtcgctt agcctgtagt gtgtcgcaac
     4501 gggataataa aacagccaca tttgacttca gcgcctgttc tttagaatgg caaaacaccg
     4561 tcgcccaggc gatcagtcag atcgacggcc tgaaaacaac acagctacca tcaccagtaa
     4621 tggctgtact cacggcactt gaaatgaaat gcacaagata taaagtgcgt gaggatgtta
     4681 tggatcagat cgtccaggaa ggcggtctgg aatatgctac tgatgtaata atacaccttc
     4741 aacagattga tattaaatgg gattatgcga ataatgtcat tattattcta ccgtctggca
     4801 ttgcacctga ctacttggag caatattcca gatttgaatt acgcctacgt aaacatttat
     4861 cactggcgga agagtctctc tggcaaaaat gtgcacaaaa acttattgcc gcaattccac
     4921 atattccaga atggcggcaa ccattaattg ctttgttatt acccgaaaaa ccagaaattg
     4981 cacatgaaat tgcccagcgt ctactggggc aaaaaaaatt accctcgctt gagtggttaa
     5041 aaatagtggc gactgatgag cacattcttg cctcattaga aaaatatcac gagccatatg
     5101 ccatttttga tgattactat tgtggtgcga tatggtcagc caccgtatta caggagcaag
     5161 gtgttgcagc cctgccccga tttgctccct atgccgcaag tgactactgc gccgatgtgt
     5221 tgcgtcatat caatcatccg ttcgcattaa cactgcttat acgtgtagcc gggcatacta
     5281 aacgctgtca cgatcggatg acgaaagcca ttgctgcgtt cccacatgca gcaatggcgg
     5341 cactgacgga acttcttggg caaaaagaag agaacagttg gcgcattatg ctaatgacaa
     5401 tgcttatctc acaaccagca ctggcagaac aggtcattcc ctggctctcg acacccgcag
     5461 ttgccgtact gaaatcatgc cagcaacaac tgacacagcc ctcaaaccat gccagcgccg
     5521 atctactgcc agccgtagta gtctcccctc cctggctttc gaaaaagaaa aaatcgccga
     5581 ttccggtgct ggatttagcg ccattaggca ttgagccaat ctgttatctg acagaagaaa
     5641 tcagtaatca acttttggcg aaatatatct ggtattcaaa acacatcacg gttagccatg
     5701 aagaaagtac taccaacctg ttggcaagga tgggttttca acgacggatc gctggtacat
     5761 atattaaagc tcccgaagcg gtagttgagg catggctaaa tgaagattat tcaaccttac
     5821 taagtgaatt taaggtgttt cattcaccta ccgggcatta ttggcagttg gggattttga
     5881 caacattgcc gctggagaaa gcagtaaaag catggaatgc ccttacccta tctccacata
     5941 ccgataccga atacgccatg ttacattttg gactcaaagg gttacctagg ttagtaaact
     6001 cacttgcacg ctatccacaa gaagccttgc ccatcacgaa ttacttcgca gcgagtgagc
     6061 tggctcctgc cgtcgcccgt gccttcaaca aactgaaaac gctacgcgaa aacgcccgta
     6121 gctggctgtt gaaatacccg gaacatgccc ttaccggcct gctgcctgcg gcgctcggca
     6181 aagccggtga agcacaggat aacgcccgcg ctgccttgcg tatgcttacc gaaaacggtc
     6241 atcagccatt actgcaagaa atcgcccgac gttataacca gccggaagta accgatgcgg
     6301 tgaacgctct gcttgcgctc gatcccttag ataatcaccc gacaaaaatc cccactcttc
     6361 cggcctttta tcagccatcg ctctggacgc gcccggtatt aaaagcaaat gcccaatcac
     6421 tgccagatag cgccctcctc cacctcggtg aaatgctccg cttccctcag gaagaggctc
     6481 tgtatccggg attattgcag gtgaaagacg tctgttccgc cgactcactg gcgggatttg
     6541 cctgggatct gtttaccgcc tggcagaccg ctggcgcgcc gtcgaaagag agttgggcgt
     6601 tcactgcgtt aggcgttctc ggtaacgatg acaccgcccg caaactgacg ccattaatac
     6661 gcgcctggcc tggtgaatcc cagcataaac gcgccaccgt tgggttggat attctcgctg
     6721 ctatcggtag tgatatcgcc cttatgcagc ttaacggcat cgcccagaaa ctgaaattca
     6781 aagcattaca ggagcgggca aaagaaaaaa ttgccgacat tgccgagagc cgcgaactca
     6841 cggtggcgga gcttgaagat cggttagcac cggatctcgg tctggatgat aacggttcgc
     6901 tgctgctgga ttttggccca cggcagttca ccgtcagctt tgatgaaacc ttaaaaccgt
     6961 ttgtgcgtga tgtttccggc agccgcctga aagacctgcc caaaccgaac aaaagcgatg
     7021 atgaatcgcg gtcgaacgat gcggttaacc gctacaaatt gctgaaaaaa gatgcgcgta
     7081 ccgtcgccgc ccagcaggta gcaaggctgg aatccgccat gtgcctgcgc cgccgctggt
     7141 cgccggaaaa cttccagctc ttcctggttg agcatccgct ggttcgccac ttaacccgcc
     7201 gtctgatttg gggcgtttat agcgccgaaa accagctact ggcttgcttt cgcgtagcag
     7261 aagataacag ctacagcacc gctgacgatg atcttttcac cctgccggaa ggcgatatct
     7321 ctatcggcac tcctcacgtt ctggaaatat caccaacgga tgctgccgcc tttggtcagc
     7381 tttttgccga ctacgaactg ctaccaccgt tccgccagct cgaccgtaac agctacgccc
     7441 tgacagaagc cgagcgcaat gccagtgaac tgacccgctg ggcaggcaga aaatgcccga
     7501 gtggtcgggt catggggctg gcgaataaag gctggataaa gggcgaacca caggatggag
     7561 gctggatcgg atggatgatc aaacctttgg gtcgctggtc gttaatcatg gaaatcgacg
     7621 aaggttttgc ggtgggtatg tcaccagccg aacttagcgc tgagcagctc ttaagcaagc
     7681 tgtggttatg ggaaggcaaa gcggaaagct atggctgggg gagtaattca acacaggaag
     7741 cgcagttctc cgtactcgat gccatcaccg ccagcgagct aattaacgat attgaagccc
     7801 tgtttgaata aggaaagagc atggacaagg aattaccgtg gctggcggat aacgcccaac
     7861 tggaactgaa atataaaaaa ggcaaaacac cactcagtca tcgcaaatgg ccgggcgaac
     7921 cggtgcctgt tatcactgaa agcatcattc agacactggg tgatgaattg ctacaaaaag
     7981 ctgagaagaa aaaaaacatt gtctggcgtt atgaaaattt ttcactggag tggcagtccg
     8041 ccatcacgca ggccatcaac ttgatcggcg aacacaaacc ctcaatcccg gcccggacaa
     8101 tggcggcgct agcctgtatc gcgcaaaatg acagccaaca gttgctcgac gaaatcgtcc
     8161 aacaagaggg gctggaatat gcgactgagg tggtgattgc acgccagttt attgcgcggt
     8221 gttatgagag tgatcctctg gtagtgacat tgcagtatca ggacgaggat tatggctatg
     8281 gttatcgctc agaaacctat aacgaattcg atctccgact gcgtaagcat ctctctctgg
     8341 cagaggaaag ctgctggcag cgttgcgccg acaaactcat tgccgcacta ccaggaataa
     8401 ccaaagttcg ccgccctttt attgcgctga tcctcccgga aaaaccagaa atagccaatg
     8461 agttggtagg ccttgaatgc ccgcgaactc attttcattc taaggagtgg ttaaaagttg
     8521 ttgctaatga ccccacagcg gtgagaaaac tcgagcacta ctggagccag gatatattta
     8581 gcgatcgaga agccagctac atgtcgcatg aaaaccactt cggctacgcg gcctgcgccg
     8641 cccttttgcg cgaacaagga ctggcagcca ttccgcgcct cgcgatgtat gcccataaag
     8701 aagattgcgg cagtctgctg gtacaaatta accatccgca agtcatccgc accttgctac
     8761 tggtggctga taaaaacaaa cccagcctgc aacgtgtagc taaataccat aaaaacttcc
     8821 cccatgcgac gctcgccgca ctggcagaac tgctggcgtt aacagaacca ccagcccgcc
     8881 caggttatcc aatcatcgaa gacaaaaagc tgcctgcaca gcaaaaagca cgcgatgaat
     8941 actggcgtac gctgttacaa acgctgatgg catcgcagcc acaactggca gaagagatga
     9001 tgcagtggtt aagtactcaa gccagggcag tgctgaatag ttatttatcg gcaccgccca
     9061 aaccggttat tgatagtacc gataacagca atctgcctga aatcctcgtt tcaccaccgt
     9121 ggcgtagtaa gaaaaaaatg acagctccac gtcttgattt ggcaccgctc gaattaacgc
     9181 cgcaagttta ctggcaacct ggcgaacgag agaggcttgc cgccactgag cctgcccgtt
     9241 atttcagcac ggaatctctt gcggaacgca tggaacaaaa aagtgggcga gttgtattac
     9301 aggaactggg ttttggggat gatgtatggc tgtttttgaa ttatatactc cccggaaaac
     9361 tggatgctgc acgcaattca ctcattgttc agtggcatta ctaccagggg cgggttgaag
     9421 agatcctgaa tggctggaac tccccggaag cacaattagc agaacaggcg ctccgcagcg
     9481 gtcacataga agcgttaatt aacatatggg aaaatgacaa ctactcacgt tatcgtccgg
     9541 aaaagagtgt ctggaacctg tatttattgg cacagttgcc gcgtgagatg gctttgacct
     9601 tctggctgcg tatcaatgag aaaaaacatc tgttcgcggg tgaggactat tttctcagta
     9661 tcctcgggtt ggatgcgcta ccaggtctga tgttggcttt ttcacatcgt ccaaaagaaa
     9721 catttccgtt aattttaaat ttcggcgcaa cagaactggc cctgcccatt gcccgcgtct
     9781 ggcaccgttt tgcgggccag cgtaatctgg ctcgccagtg gattttacaa tggccggaac
     9841 atacggctac tgcacttatc cctcttatct tcaccaaatc tagcgataaa agcgaagctg
     9901 cattacttgc cctgcgttta ctttacgaac atgggcatgg cgaattgcta caaactgtag
     9961 ccaatcgttg gcagcgtaca gatctatggc ctgccctgga acaattactt aaacagagtc
    10021 caatagagat ttacccggca cgcattccaa aagcccctga tttctggcat ccgcaaatgt
    10081 ggtccaggcc gcgccttatc actaataatc aacctgttac cgatgacgct ctggaaatta
    10141 tcggcgaaat gctgcgcttt acccaggggg gacgttttta tagcgggctg gaacaactga
    10201 aaacgttctg ccagccacaa acgctggcag cttttgcctg ggatctcttc actgcgtggc
    10261 aacaagctgg tgcccccgta aaagacaact gggcatttct ggcgttaagt ctctttggtg
    10321 acgaaagcac ggcacgggat ctaacgacac agatcctcgg ctggccacaa gaaggcaaat
    10381 ctgcccgtgc tgtcagtggc ctgaacatcc ttaccctgat gaataatgat atggcgctga
    10441 tacagctgca tcatatatcg caacgggcga aatcccgccc cttacgtgat aacgcggcgg
    10501 aatttcttca ggtggtcgca gaaaatcgcg ggctaagcca ggaagagcta gcggacagat
    10561 tagtcccaac cctgggcctt gatgatccgc aggcgttgag ttttgatttt ggtccccggc
    10621 agtttaccgt tcgcttcgat gaaaacctca acccggttat ctttgatcag caaaacgttc
    10681 gccagaaaag cgttccccgg ttgcgcgccg atgacgatca actgaaagcg cccgaggcac
    10741 tggcccgact aaaagggcta aaaaaagatg ccactcaggt gagcaaaaac ctgctcccgc
    10801 gtcttgaaac ggccctacgt accacccgac gctggtcgct ggcagatttt cattctctgt
    10861 ttgttaatca tccctttacc cgtctggtta cccagcgatt aatatggggg gcttatccgg
    10921 caaatgaacc gcgttgttta ctcaacgcct ttcgtgtggc cgcagagggg gagttctgca
    10981 atgcgcaaga tgagccaatt gacctgcctg cggacgctct gattggcatt gcccacccgt
    11041 tagaaatgac agtagaaatg cgcagtgaat ttgcacagct ttttgccgat tacgaaatta
    11101 tgccgccttt tcgccagttg gcgcggtgca cggtgctgct cacacctgat gagtcaacca
    11161 gtaacagcct gactcgctgg gaaggtaaat ccgctaccgt tgggcagctt atgggaatgc
    11221 gatacaaagg ctgggagtca ggctatgagg atgcatttgt ctatgacctg ggcgagtacc
    11281 ggctggtcct taagttttca cccggtttta accactacaa tgttgatagc aaagcgctaa
    11341 tgagcttccg ttctcttcga gtgtaccgtg acaataaatc cgtcactttt gccgaacttg
    11401 atgtgtttga tttgagtgag gcgttaagcg cacctgacgt cattttccat taaaacacag
    11461 cgggcagtga tgcgactgcc cgttatctac acgacttacc agcggggaaa gcgatgatcg
    11521 tgcaaaaaga gctagttgct atttacgatt atgaggtccc tgtacctgaa gatccgtttt
    11581 ccttcagact tgagatccat aaatgctctg aattatttac aggttccgtc tatcgactgg
    11641 agcgattccg gctacatcca acatttcatc aacgtgatcg agaagatgct gacccgctaa
    11701 taaatgatgc gttgatttat ataagagatg agtgtattga tgagcggaaa ttacgaggtg
    11761 aatcacctga aactgtaata gcaattttta atcgtgaact acagaatata ttcaaccaag
    11821 aaatagaata atatactcta gataattaaa gtcgcagcac atgcaacttg aagtatgacg
    11881 agtatagctt tcttaaaggg ggaaacggta ttccccctta gatgcgtttc aatttgcccg
    11941 ttacttcaac aaatcaatca accgtaacag agccactaat aactccagta cggcaatgag
    12001 cacggttaac ttttgtgtca gcgtgtccat ccgctctcct tattaaggag cgcaacacca
    12061 gcaccttacc cttacactgc ctgttgatac tggtttacgt atgttggtac gaaacgttgc
    12121 tctccggatc agggcaccgc ctgcggcacc acccgtccgg cagccaggac ttaaaattgg
    12181 tccgatccgg cacaacctcc caacgggcag gattataaat ctcactcact gaattgcaat
    12241 ggagtgtgga tatggttata atttattcgt tggtacgaaa tgttgcacaa tcagcaagtt
    12301 agcgtcactg tagtaaataa aagaacctgc ctcaccagca ggttttttta ttttctgcga
    12361 tctactttcc agatattttt tgccaatagc ccaacataa
//
LOCUS       DBANUU010000128        12123 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000128 DBANUU010000000
VERSION     DBANUU010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.129"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            270..1502
                     /gene="mdtM"
                     /locus_tag="ACIE68_003672"
     CDS             270..1502
                     /gene="mdtM"
                     /locus_tag="ACIE68_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFX5909927.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIRLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAIMGFISFVGLLLAMPETVKRGAVSFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIIPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGLIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            1543..2823
                     /gene="yjiN"
                     /locus_tag="ACIE68_003673"
     CDS             1543..2823
                     /gene="yjiN"
                     /locus_tag="ACIE68_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFX5909928.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPVLLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKIDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRVHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADIDSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLSNF"
     gene            2939..4090
                     /gene="yjiM"
                     /locus_tag="ACIE68_003674"
     CDS             2939..4090
                     /gene="yjiM"
                     /locus_tag="ACIE68_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFX5909929.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEIL"
     gene            4100..4867
                     /gene="yjiL"
                     /locus_tag="ACIE68_003675"
     CDS             4100..4867
                     /gene="yjiL"
                     /locus_tag="ACIE68_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFX5909930.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLEPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVIPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            4864..5121
                     /gene="ytiA"
                     /locus_tag="ACIE68_003676"
     CDS             4864..5121
                     /gene="ytiA"
                     /locus_tag="ACIE68_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFX5909931.1"
                     /translation="MKEYLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            5186..6046
                     /gene="yjiK"
                     /locus_tag="ACIE68_003677"
     CDS             5186..6046
                     /gene="yjiK"
                     /locus_tag="ACIE68_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HFX5909932.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLAGEVIGEMSLTKGSRGFSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            6114..7292
                     /gene="yjiJ"
                     /locus_tag="ACIE68_003678"
     CDS             6114..7292
                     /gene="yjiJ"
                     /locus_tag="ACIE68_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5909933.1"
                     /translation="MPSPSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     LPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMMLIALTLLMPSKKHAITPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(7305..7859)
                     /gene="kptA"
                     /locus_tag="ACIE68_003679"
     CDS             complement(7305..7859)
                     /gene="kptA"
                     /locus_tag="ACIE68_003679"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFX5909934.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEW"
     gene            8109..8792
                     /gene="yjiH"
                     /locus_tag="ACIE68_003680"
     CDS             8109..8792
                     /gene="yjiH"
                     /locus_tag="ACIE68_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFX5909935.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            8789..9250
                     /gene="yjiG"
                     /locus_tag="ACIE68_003681"
     CDS             8789..9250
                     /gene="yjiG"
                     /locus_tag="ACIE68_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFX5909936.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            9263..10435
                     /gene="iadA"
                     /locus_tag="ACIE68_003682"
     CDS             9263..10435
                     /gene="iadA"
                     /locus_tag="ACIE68_003682"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFX5909937.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            10500..11411
                     /gene="hypT"
                     /locus_tag="ACIE68_003683"
                     /gene_synonym="qseD"
     CDS             10500..11411
                     /gene="hypT"
                     /locus_tag="ACIE68_003683"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFX5909938.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(11404..11796)
                     /gene="iraD"
                     /locus_tag="ACIE68_003684"
     CDS             complement(11404..11796)
                     /gene="iraD"
                     /locus_tag="ACIE68_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFX5909939.1"
                     /translation="MMRQSRQAVLPEISGNKTSSLRKSICSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYSHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(11793..11876)
                     /gene="idlP"
                     /locus_tag="ACIE68_003685"
     CDS             complement(11793..11876)
                     /gene="idlP"
                     /locus_tag="ACIE68_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFX5909940.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(11992..>12123)
                     /locus_tag="ACIE68_003686"
     CDS             complement(11992..>12123)
                     /locus_tag="ACIE68_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909941.1"
                     /translation="AEINNFTPELSSGGDKYFHLRNYSEYSEYTSGFFLSLMIFIKS"
BASE COUNT         2719 a         3059 c         3160 g         3185 t
ORIGIN      
        1 aattttaatt acacttaagg tgtatatttt ctatgcaagc catcaattaa agaggtgtaa
       61 tgtgctgatg actatttgta atcgttatac attctgacct ggagtcagaa ggtatttctc
      121 tgtcggtgtg ttcacaggca gtgtggttga ttacatgaaa ttcagtacat ttgcagtctc
      181 gttgcccttc tcgcctgcct ttcgtcatta ccgacggtat tgaatttcgt tttgcccgtt
      241 ggggttctcc ggacaaggag ttgtttgtta tgccacgttt ttttgcccgc catgccgcca
      301 cgctgttttt cccgatggcg ttgattttgt atgattttgc cgcgtacctg tcgacggacc
      361 tgattcagcc ggggatcatt aatgtggtcc gtgattttaa tgccgatgtc agtctggccc
      421 cggcagccgt cagtctctat ctcgcgggcg gtatggcgtt acagtggctg ctggggccgc
      481 tttccgacag gattggccgc aggccggtgc tgattaccgg ggcgctaata tttacccttg
      541 cctgcgctgc gacaatgttc acaacgtcta tgacacagtt tcttatcgcg cgtgcaattc
      601 agggcaccag tatctgtttt attgccaccg ttggttatgt cacggtgcag gaggcgttcg
      661 gccagacaaa agggatcagg ttgatggcga ttatcacctc catcgtactg attgcgccga
      721 ttatcggccc gctttccggc gcagctctga tgcactttgt acactggaaa gtcctttttg
      781 ccatcattgc gattatgggt tttatctcat ttgttggctt actgctggcg atgccagaga
      841 cggtgaagcg cggcgcggtt tcatttagcg ccaaaagcgt cttgcgcgat tttcgtaatg
      901 tcttttgcaa tcggctgttc ctctttggtg cagcaaccat ctctttaagc tatatcccaa
      961 tgatgagctg ggtggctgtc tcgccggtga tcctgattga cgcaggcggc ttaacgacct
     1021 cgcagttcgc ctggacacag gttccggtat tcggcgcggt gattgtcgcg aatgccatcg
     1081 tggcgcgttt cgttaaagat ccgaccgaac cgcgctttat ctggcgtgcc gtacccattc
     1141 aactggttgg cctcgcgctg ttgattatcg gcaatctgct gtcaccgcac gtctggctgt
     1201 ggtcggtgct gggcaccagt ctgtatgctt tcgggattgg tttgattatc ccgaccttat
     1261 tccgctttac gctgttttcc aataacttac cgaaagggac cgtctccgca tcgctgaata
     1321 tggtgatcct gatggtgatg tctgtctcgg tcgaaatcgg ccgctggctg tggtttaacg
     1381 gcggtcgctt gccgtttcat ctgttagccg tcgtggcggg ccttatcgtc gttttcaccc
     1441 tggcgggatt gcttaatcgc gtgcgccaac atcaggctgc cgagctggcg gaggagcggt
     1501 gatttttgcg cgatcctgcc gtcaggctct attcttaaca ttatgaataa actcattgaa
     1561 ctcagacgcg ccaaaatgtt ggcgctctct ttactgctta tcgccgctgc tacctttgtc
     1621 gttacgctgt ttttgccacc caatttttgg gtgagcggtg tgaaggcgat tgctgaagcg
     1681 gcgatggtcg gcgcgctggc agactggttt gcggtggtgg cgctgtttcg ccgcgtgccg
     1741 attccgatca tttctcgtca tacggcgatt atcccgcgta ataaagaccg gattggcgaa
     1801 aatctcggtc agttcgtgca ggaaaaattt ctcgataccc aatcgctggt ggcattgatt
     1861 cgacgtcacg aaccggtgtt gttgattggc aactggttta gtcagccaga aaacgcccgc
     1921 cgcgttggtc agcatctgtt gcagatcatg agtggttttc ttgaactgac cgatgatgcg
     1981 cgtattcagc gcctgcttaa gcgcgcagtc catcgggcga ttgataagat cgatctttcc
     2041 ggcaccagtg cgttgatgct ggagagtatg accaaaaacg atcgtcatca ggtgctgctg
     2101 gatacgctga tcgcgcagtt gattgctctt ctccagcgcg ataaatcgcg caagtttatt
     2161 gcccagcaaa ttgttcgctg gctggagagc gagcatccgc tgaaagccaa aattttgcct
     2221 actgaatggc tgggcgaaca tagcgcggag ttggtttctg acgcggtgaa ttctttgctt
     2281 gatgatatca gccgcgatcg tgtacatcag atccgccatg cgtttgatcg cgccactttt
     2341 gccctgatcg acaagttgaa aaacgatccg gaaatggcag cgcgagccga tgccgtaaaa
     2401 agttatctga aagaagatga agcttttaac cgctatctca gtgaattgtg gggggattta
     2461 cgggaatggc tgaaagcgga tatcgacagt gaagattctc gtgtgaaaga acgtatcgcg
     2521 cgggcgggtc aatggtttgg cgaaacgtta attgccgatg atgccttgcg ggcgtcgtta
     2581 aatggtcacc tggaacaagc cgcgcaccgc gtcgcgcctg agttttccgc attcctgacg
     2641 cgccacatca gcgatacggt aaaaagctgg gatgcgcggg atatgtcgcg gcaaatagag
     2701 ttaaatatcg gcaaagatct gcagtttatc cgtgtcaacg gtacgctggt cggcggttgt
     2761 attgggctaa ttttgtattt gctgtcgcag ctcccggcct tattccccct cagcaatttt
     2821 tagaaatcca tgaaaaacta agtggctaac cgcgagagag atcaaataaa ccacactaaa
     2881 agaatggtaa taatgtggcc tcttttcatt atctcccgtg gtacggggaa ggaaaatcat
     2941 gtcacttgtc accgatctac ccgccatttt cgatcagttc tctgaagctc gccagaaagg
     3001 ctttctcacc gtcatggatc tcaaggagcg cggcattccg ctggttggca cttactgcac
     3061 ctttatgccg caagagatcc cgatggcagc cggtgcggtt gtggtttcgc tctgttccac
     3121 ctctgatgaa accattgaag aagcggagaa agatctgccg cgcaacctct gcccgctgat
     3181 taaaagtagc tacggcttcg gcaaaaccga taaatgcccc tacttctact tttcggatct
     3241 ggtggtcggt gaaaccacct gcgacggcaa aaagaaaatg tatgaataca tggcggagtt
     3301 taagcccgtt catgtgatgc aactgccaaa cagcgttaag gacgatgcct cgcgtgcatt
     3361 atggaaagcc gagatgctgc gcttgcaaaa aacggtagaa gaacgttttg ggcacgagat
     3421 tagcgaagat gctctgcgcg atgccattgc gctgaaaaac cgcgaacgtc gcgcactggc
     3481 caacttttat catttggggc agttaaatcc tccagcgctt agcggcagcg acattctgaa
     3541 agtagtctac ggcgcaacct tccggttcga taaagaggcg ttgatcaatg aactggatgc
     3601 aatgaccgcc cgcgttcgtc agcagtggga agaaggccag cgactggacc cgcgcccacg
     3661 tattttaatc accggctgcc caattggcgg cgcagcagag aaagtggtgc gcgcgattga
     3721 agagaatggc ggctgggttg tcggttatga aaactgcacc ggggcgaaag ccaccgagca
     3781 atgcgtggcg gaaacgggtg atgtgtacga cgcgctggcg gataaatatc tggcgattgg
     3841 ctgctcctgt gtttcgccga acgatcagcg cctgcaaatg ctcagccaga tggttgaaga
     3901 atatcaggtc gatggcgtag ttgatgtgat tttgcaggcg tgccatacct acgcggtgga
     3961 atcgctggcg attaaacgtc atgtgcgcca gcagcacaac attccttata tcgctattga
     4021 aacagactac tccacctcgg atgtcgggca gctcagcacc cgtgtcgcgg cctttattga
     4081 gatactgtaa ggagtggcag tgacatattc gattggcatt gattccggct caaccgccac
     4141 caaagggatc ttactggctg acggcgtgat tacgcgccgt ttcctcgttc caacaccctt
     4201 tcgcccggca acagcaatta ctgaagcctg ggaaactctg cgcgaagggt tagagacaac
     4261 gccgtttctg acgctcaccg gctacggacg gcaactggtg gattttgccg ataaacaagt
     4321 gacggaaatc tcctgtcacg ggctgggcgc acggtttctt gagccagcaa cgcgcgcggt
     4381 aatcgacatc ggtggtcagg acagcaaagt gattcagctt gatgatgacg gtaacctgtg
     4441 cgatttcctg atgaatgaca aatgcgcggc gggcaccggg cgtttcctgg aggtgatctc
     4501 gcgcacgctt ggcaccagcg tcgagcaact cgacagcatt accgaaaatg tcataccgca
     4561 cgccatcacg agtatgtgca cagtgtttgc tgaatcagaa gtgatcagcc tgcgctctgc
     4621 gggcgtagcg ccagaagcga ttctcgccgg agtgattaac gcgatggcgc ggcggagcgc
     4681 caatttcatt gctcgtctct cctgtgaagc gccgattctg tttactggtg gcgttagcca
     4741 ctgtcaaagg tttacccaca tgctggaaag ccatctggga atgccggtac aaactcaccc
     4801 tgatgcgcaa ttcgctggtg caattggcgc ggcggtgatc ggtcagcggc agagaaaacg
     4861 cgcatgaaag agtatctgtt tttatttcac tccacggtcg gcgtcataca aacccgcaag
     4921 gcgttgcagg cagcgggcat gacttttcgc gtcagcgata ttccccgtga tttacgcggc
     4981 ggctgcgggt tatgcatttg gctgacctgt ccgcctggcg aggaaattca atgggtgatc
     5041 cctgggcata cggaatcagt ttattgtcag caggatggtg gctggcgctg cattgcacat
     5101 tacgggattt ctccgcgtta aggattcatt aagatcgcta cgtatacttc tcgatatctt
     5161 attgatttgc cttaaaggga ttaccgtgac aaaaagtatt tcgttaagta aacgcatctt
     5221 cgttattgtg attctttttg tcattgtggc agtgtgtact ttcttcgtgc aatcttgcgc
     5281 ccgtaaaagc aatcatgcag catcatttca gaattaccac gccaccattg acggcaaaga
     5341 aattgcaggc ataacgaata atatctcttc gctgacctgg tcggcacaaa gcaatactct
     5401 ttttagcact atcaacaaac ctgccgccat tgttgaaatg accaccaatg gcgatttgat
     5461 ccgcacaatt cctctggatt ttgttaagga tctcgagacg atcgaataca ttggcgataa
     5521 tcagtttgtc attagcgatg agcgcgatta tgccatttat gtgatttcac tgaccccaaa
     5581 ctcggaagta aaaatcctca aaaaaataaa aatcccgctg caggaatccc caaccaattg
     5641 cggttttgaa ggattggcct attctcgcca ggatcacact ttctggtttt ttaaagagaa
     5701 aaacccgatt gaggtctaca aagttaacgg gctgttaagt agcaatgaat tgcacatcag
     5761 caaagacaag gcactacaac ggcaatttac tttagatgat gtgtccggcg cagaatttaa
     5821 ccaacaaaaa aatacgcttc tggtgctgtc tcatgaatct cgcgcactcc aggaagtaac
     5881 gctggctggc gaggttatag gtgaaatgtc actaaccaaa ggcagtcgag gtttctcgca
     5941 taacatcaaa caggccgaag gggttgcgat ggatgcctca ggtaatattt atatcgttag
     6001 cgaaccaaat cgtttttatc gctttacccc tcaatcatcg cattaacagg ctggcatgtt
     6061 tcaccttttt gttattaact tgttaacaac aaaaatgaca aacaaggtta aacatgcctt
     6121 cgccctcgca tcccgtagaa cgcttttctt tcagcaccgc gcttttcggg atgctggttc
     6181 tgaccttagg tatgggttta ggccgcttcc tttatacgcc tatgttgccc gtcatgatgg
     6241 cggaaggatc gttttcattt agccagctct cgtggattgc cagcggcaac tatgcggggt
     6301 atctggctgg cagtctgcta ttttcgtttg gcgcatttca ccagccatcg cgcctgcgcc
     6361 catttctgtt ggcttctgcc ctggcgagcg ggttgttgat cctcgcaatg gcatggttgc
     6421 tgccatttat tctggtgtta ttgattcgcg tcctggcggg tgtcgccagc gccggtatgc
     6481 tgatttttgg ttcgacgctg attatgcagc acacgcgcca tccttttgtg ctggcagctt
     6541 tgttttctgg cgttggcatt ggcatcgcac tgggcaatga atatgttctg gcaggcctgc
     6601 attttgacct ctcctcgcaa acgttatggc aaggcgccgg ggcgctttct ggcatgatgc
     6661 tgattgcact tacgctttta atgccctcga aaaaacacgc catcacacca atgccattgg
     6721 caaaaacgga gcaacagata atgagctggt ggttactggc tattctgtac ggcctggcgg
     6781 gatttggtta tatcatcgtc gcgacttacc tgccgctcat ggcgaaagat gcaggctcac
     6841 cattgttaac cgcccatctc tggacgttag tcggcttatc aattgtgcct ggttgctttg
     6901 gctggctatg ggcagcaaaa cgttgggggg ctttgccctg cctgacggcg aatttgctgg
     6961 tgcaggctat ctgtgtgctg cttactctcg ccagcgactc gcctctcttg cttatcatca
     7021 gcagtcttgg ttttggcggt acctttatgg gcacgacatc tctggtgatg actatcgccc
     7081 gccaactgag cgtaccggga aatctcaatc ttttaggctt tgtgacgctc atttacggca
     7141 ttggacaaat cctcggcccg gcgctgacca gtatgctcgg caatggaaca tcggcgcttg
     7201 ccggcgccac tctctgcggc gcggcggcgt tgtttattgc tgcgcttatc agcacagtac
     7261 aattatttaa gctgcaagtg gtcacttcat gaggagtgtt tcccttacca ctcaagaaat
     7321 tcgaccgcaa ccgttgatgt cagccagacc ccgttttccg cttgccagaa agggaggcct
     7381 cgtttcgcca tttcctgtgc tttgacggtt aaaataaccg gcgatccgtg tcttgctccc
     7441 actttgcgtg ccgttgcttc atcagcagag agatgtacgt agtgacgctc acctgcaatc
     7501 agcccctgtt tttttatctc gtcaaggaaa cggcttgccg taccgtggta gagaaactgt
     7561 ggcggtgttt tttcggcaaa ggaaatagcg acctgcgaag tcgaatgccc ctgaaccgcg
     7621 cggatgcacc taccatcact ggaataacta aaacgctttt tatcgctggt ggcaaccacg
     7681 gtatccagca atgcacgggt taaccgcttg cccgcttttt gggcacaaag tatgagctta
     7741 tcgatatccg cccatccttc acggtccagt acgataccaa tcgcctccgg tttgtgtcgg
     7801 aggacaaaac ttaaaaactt gctcgtatcc gccagctctt tttcgttgta ctttgccatc
     7861 gcgttttcgt ccctggatta tctgtttttt atttactatg tcattgtcac tgggtttatg
     7921 aattttctgc aactcaatcc tgcgtagaac cttttccaca cagcgtaatt ttaccgctat
     7981 gcaaaaaacg catagtcaga aacatacttg cattttcccc atacccacat ggctgataag
     8041 gtttttcagc tccaggaatt ttcctggctc aagcaacaca caaacacaaa aaaacaatgg
     8101 ggattgttat gacacaacaa ggggatgctg tagccgggga gctggcgaca gaaaaagtgg
     8161 gcattaaagg gtatctggct ttttttctca ccataatctt tttttccggt gttttttccg
     8221 ggacagacag ttggtggcgc gtttttgatt tttccgttct gaacggatcg tttgggcaac
     8281 tgcctggtgc gaacggagcc accacctcgt ttcgcggcgc gggtggcgcg ggggctaaag
     8341 atggctttct ctttgcgctg gagctggcac cgtcagtcat tctctctctg gggattattt
     8401 caattaccga tggtcttggc ggattacgcg ccgcgcagca gttaatgacg ccagtgctaa
     8461 aaccgttgtt gggcattcca gggatctgct cgctggcgct gatcgctaac ctgcaaaaca
     8521 ccgatgccgc cgcaggtatg accaaagagc tggcgcagga aggtgaaatt accgaacgcg
     8581 ataaagtgat tttcgccgca tatcaaacca gcggtagcgc cattatcacc aactacttct
     8641 cttccggcgt cgcggtgttt gcctttctgg gcacgtccgt aatcgtacct ttagcagtca
     8701 tcctggtgtt taaatttgtc ggagccaaca ttttgcgcgt ctggcttaac ttcgaagaac
     8761 gtcgcaaccc aacgcaagga gcgcaagcat gacaactcag gtccgcaaaa acgtcatgga
     8821 tatgtttatc gatggtgcgc gccgtggctt tactatcgcc acgaccaacc tgctgccaaa
     8881 cgtggtgatg gcgtttgtca tcattcaggc gctgaaaatt accggcctgc tcgactgggt
     8941 ggggcatatc tgtgaacccg taatggcgct gtggggatta cccggtgaag cggcaaccgt
     9001 ccttttagcc gcattaatga gtatgggtgg cgcagtcggt gttgccgcca gtctggcaac
     9061 cgctggcgca ttaaccggtc acgatgtcac cgttttactg cccgcgatgt atttaatggg
     9121 caacccggtg caaaatgtcg gtcgctgcct ggggacagcc gaagtgaatg ccaaatatta
     9181 cccgcatatc attacggtct gcgtgattaa cgcattgctg tcgatctggg ttatgcagct
     9241 tattgtttaa caaggagtta ccatgattga ttataccgca gccggtttta ccctgctgca
     9301 gggagcgcat ttgtatgcgc cggaagatcg gggaatttgc gatgtcctcg tcgctaacgg
     9361 caaaattatc gccgttgcca gtaatatccc ttctgacatt gtaccggact gcacggttgt
     9421 cgatctcagc gggcagatcc tctgcccagg ttttattgat caacacgtcc atttgattgg
     9481 cggtggcggc gaagcaggtc ccacgacgcg cacgccggaa gtggcgttaa gtcgtctgac
     9541 ggaagcaggc gtcacgtcag tggttggtct gctgggcacc gactctatct ctcgccaccc
     9601 ggaatccctg ctcgccaaga cccgtgcgct caatgaagaa ggcatcagcg cctggatgct
     9661 gaccggcgct tatcatgtcc cttcccgcac tattacgggt tccgtggaaa aagacgtggc
     9721 gattatcgat cgcgtgattg gcgtgaaatg cgccatctct gatcaccgtt ctgccgcacc
     9781 ggacgtttat cacctggcta atatggcggc ggaatcccgc gttggcggtt tgctcggcgg
     9841 taaacctggc gtcaccgtgt tccacatggg cgacagcaaa aaggcgttac agcctgtcta
     9901 tgacctgctg gaaaactgcg atgtgccgat tagcaagttg ctgccgaccc acgttaaccg
     9961 caacgttccg ttgtttgagc aggcgctgga gttcgcgcgc aaaggcggca ccatcgatat
    10021 caccagcagc attgacgaac cagtcgcccc tgccgaaggt attgcccgcg ccgttcaggc
    10081 gggtattccg ctggcacgcg tcaccctcag ctccgacggc aacggtagcc agccgttctt
    10141 cgatgacgaa gggaatttaa cccatatcgg tgttgccggt tttgaaacgt tgctggaaac
    10201 cgtgcaggtg ctggtcaaag attatgattt cagtatcagc gatgccctgc gcccgctcac
    10261 cagtagcgta gccggtttcc ttaacctgag cgggaaaggc gaaattctgc caggcaatga
    10321 cgcagactta ctggtcatga cgccagaatt acgcattgag caggtatacg ctcgcggcaa
    10381 gctgatggtc aaagacggca aagcctgcgt gaaaggaacg tttgaaacgg cataaacggt
    10441 tgtcagccgc ccgaatggac gtagaatgcc cgcaagggcg gctgacagag taaaacgtaa
    10501 tggatgactg tggtgcggtt ttgcataata ttgaaactaa atggctttat gattttctga
    10561 ccctggaaaa atgccgcaat ttttcccagg cagcagtcag tcgcaacgtc tcgcaaccgg
    10621 cattcagccg ccgcatccgt gcgctggaac aggcgattgg cgttgaattg tttaaccgcc
    10681 aggtgacgcc gctgcaactc tcggaacaag gtaaaatatt ccattcgcag atccgccatc
    10741 tgttgcaaca gttagagagc aaccttgcag aactccgtgg tggcagcgat tacgcgcaac
    10801 gcaaaattaa aatagccgct gcacactctc tttccctcgg gctgttaccg tccattatca
    10861 gccagatgcc gccgctcttt acctgggcaa ttgaagctat tgatgtcgat gaagcggtcg
    10921 ataaactgcg tgaagggcaa agtgactgta ttttttcctt tcacgacgaa gatttgctgg
    10981 aagcgccgtt tgaccacatt cgcttatttg aatctcaatt gttccctgtc tgcgccagtg
    11041 acgaacacgg agaagcactt tttaacctcg cgcagccaca ctttccgtta ctgaattaca
    11101 gccgcaactc ctacatgggg cgattgatta atcgcaccct gacgcgccac agtgagttaa
    11161 gtttcagcac cttttttgtc tcttcgatga gcgagctttt aaagcaggtt gccctcgacg
    11221 gctgtgggat tgcctggctg ccggagtacg ccatccaaca agaaattcgc agcgggcagc
    11281 tcgttgtact gaatcgggac gaactggtga tcccgattca ggcctatgca taccgaatga
    11341 atacccgcat gaatccagtt gccgaacgct tctggcgtga actgcgcgag ctggagattg
    11401 tacttagctg acattctcga gcgtcgcact acgccaaccg ccattatccc agaccagatc
    11461 aaacgttagc ggttcatcgt cgtggtacag cattatctca aggctgatcg ccagcgtgtg
    11521 gctgtacgct tctttcagat tgacctgcaa cgccgcaatc cgtggctcat gaaataacag
    11581 tgcagaacgc agctgacggc aataccagct ctggagatgt ttgtcgctct ggttatgcac
    11641 ttgccactcg ggcatgcctg aaacaagtaa tgacggtaat gccgaatgag gagaattaaa
    11701 aagagtgagg agatcgctac atattgattt acgcagcgat gacgttttat tgcctgaaat
    11761 ttcaggtaaa acagcctgac gtgattggcg catcattttg cgcactcctg acgtttagca
    11821 acatatgcgg cttgccctga acaccgggca ccactgtatt gatgctcatt ttccatagcc
    11881 attccctttt gttcattaca tccatgtcag tctgagacag gcctgaatcc tgtcagcaaa
    11941 gtataaaacc ttacttgcct atgaatatct actgactttt caggaaaaaa gtcatgattt
    12001 gataaaaatc atcaaactca aaaaaaaacc gctagtatat tctgaatatt ccgaatagtt
    12061 acgtagatga aaatacttgt caccaccgct acttaattcc ggtgtgaaat tatttatttc
    12121 cgc
//
LOCUS       DBANUU010000129        11943 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000129 DBANUU010000000
VERSION     DBANUU010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.130"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(37..741)
                     /locus_tag="ACIE68_003687"
     CDS             complement(37..741)
                     /locus_tag="ACIE68_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001410974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFX5909942.1"
                     /translation="MVEEQKYPDFAKRLNELMTNKGISVTQLKSLVGVTYEMARRYTI
                     GAAKPRASVMNKLALALGVSASYLEYGVGEREGCKEMASIPNPTKPDVYRIEVLDLSV
                     SAGPGTYMLSDYVDVLYAIEFTTEHARSLFGNRSQDDIKVMTVNGDSMSPTLVSGDRL
                     FVDISVRNFQTDGVYSFVYGKTFHVKRLQMQGNKLAVLSDNPAYEKWYIDEKSQDQLY
                     VMGKALIHESIKYNRL"
     gene            848..1111
                     /locus_tag="ACIE68_003688"
     CDS             848..1111
                     /locus_tag="ACIE68_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFX5909943.1"
                     /translation="MSSQNYTEKAVKAAGKSLSEVARHFGFKSTQSVANWVINNQVPS
                     ERVLQLCELGNWSVTPHELRPDIYPNPNDGLPECYSKVSGSAA"
     gene            1140..1718
                     /locus_tag="ACIE68_003689"
     CDS             1140..1718
                     /locus_tag="ACIE68_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfL family putative regulatory protein"
                     /protein_id="HFX5909944.1"
                     /translation="MGKEPEWKVDKQPAWLVAAIRRTIADLPHGYEEAAEILGLYKSD
                     DITPAKDQLHNRLRSGGDQIFPLEWAMVLQDASGTKHVTDAIARRSNGVFVPLVDIDD
                     IDNGDINQRLMESIEWIGKHSQYLRKATADGVIDRAEREQIEENSYQVMAKWQEHLTL
                     LFRVFCAPEKSDARECAAPGVVAFCVRGETNA"
     gene            1864..1959
                     /locus_tag="ACIE68_003690"
                     /pseudo
     CDS             1864..1959
                     /locus_tag="ACIE68_003690"
                     /inference="COORDINATES: protein motif:HMM:NF025339.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            1913..2878
                     /locus_tag="ACIE68_003691"
     CDS             1913..2878
                     /locus_tag="ACIE68_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFX5909945.1"
                     /translation="MLFVGSQKHSALSIFAKTTRMSALVLCGNSGVILLSVKDHQHID
                     SAIPGRYTVQAPYKAGAGIGVLKLITEHNRAHAVFSCHEHCYAQIMVGRTGPTSVGPG
                     SVVSGNANPVRLTTSVIGVPCGEFSKKLTTGAVTMTTLPTLSQPEIAIVDGQAVTSSL
                     AVADFFSKRHDDVLKKIRILDCSPEFCARNFAETSILVRQPNGGTRKLPCYQITRDGF
                     AFLAMGFTGKRAAQFKEAYINAFNQMEKQLSKPSVPSDVAHNASVLYSYISSIHQVWL
                     QQLYPMLEKAESPLAVSLYDRINDAAFLARLIHSSLNSSEVRGRK"
     gene            2875..3099
                     /locus_tag="ACIE68_003692"
     CDS             2875..3099
                     /locus_tag="ACIE68_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909946.1"
                     /translation="MIRNIFKRFTNQTFRCPRPGQWYTTPAGHVLRVSLVDRECQKVI
                     CEPLGRNYRVSMPLIAFCSGKNMKHLGGAA"
     gene            3096..3914
                     /locus_tag="ACIE68_003693"
     CDS             3096..3914
                     /locus_tag="ACIE68_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFX5909947.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCDSGLVKKVTRKGEKGNSSNIYLLHLDGAGDSLGGSANNSL
                     SGAANSPGSAGVAPGGSAGDSPRTSHSFEPVKEPVNEPIAVGASADESVRVRSNRPEY
                     SPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGVKPETMLEGVKRYAGWVSAMGNSG
                     TQFVKQAVTFFGPDRHFEESWEVPAVSAARREDPYFKASYDNVDYSQIPAGFRG"
     gene            3911..4405
                     /gene="yfdN"
                     /locus_tag="ACIE68_003694"
     CDS             3911..4405
                     /gene="yfdN"
                     /locus_tag="ACIE68_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFX5909948.1"
                     /translation="MIMSLLNEVQKFIEAHPWCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            4405..5058
                     /locus_tag="ACIE68_003695"
     CDS             4405..5058
                     /locus_tag="ACIE68_003695"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFX5909949.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            5055..5381
                     /locus_tag="ACIE68_003696"
     CDS             5055..5381
                     /locus_tag="ACIE68_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFX5909950.1"
                     /translation="MTTLTQCQQQVLDMLISYQQERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRAAHWLH
                     ERELKV"
     gene            5378..5767
                     /locus_tag="ACIE68_003697"
     CDS             5378..5767
                     /locus_tag="ACIE68_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFX5909951.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTVGAAA"
     gene            5787..6596
                     /locus_tag="ACIE68_003698"
     CDS             5787..6596
                     /locus_tag="ACIE68_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFX5909952.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVHELTNTQNLGVDPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            6604..7593
                     /locus_tag="ACIE68_003699"
     CDS             6604..7593
                     /locus_tag="ACIE68_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFX5909953.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            7607..8359
                     /locus_tag="ACIE68_003700"
     CDS             7607..8359
                     /locus_tag="ACIE68_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFX5909954.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCYGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKICGRCNGNRFSRLPTTLARHHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAETQLRKVTR"
     gene            8649..8891
                     /locus_tag="ACIE68_003701"
     CDS             8649..8891
                     /locus_tag="ACIE68_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909955.1"
                     /translation="MVERCSVCEQSLNYSREVEQEGVEYKSCPNCSADAGVHVFYKTI
                     DFGYRDMGDGRHIVQSWCPACRSGEKPSIPPAFKCC"
     gene            9023..9364
                     /locus_tag="ACIE68_003702"
     CDS             9023..9364
                     /locus_tag="ACIE68_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFX5909956.1"
                     /translation="MKMHNDPHSWSDLLELLQSWWHGDTPLGAVIMSIVMAGLRIAYF
                     DGGGGWKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMR
                     VIGNKFGGSKE"
     gene            9368..9844
                     /locus_tag="ACIE68_003703"
     CDS             9368..9844
                     /locus_tag="ACIE68_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFX5909957.1"
                     /translation="MQVLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGGLFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADNLIAKFKEAGGTVREIEV"
     gene            10061..10252
                     /locus_tag="ACIE68_003704"
     CDS             10061..10252
                     /locus_tag="ACIE68_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588452.1"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFX5909958.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPVWVMQPPP
                     DLLTPLNGIISPSENESTQ"
     gene            10377..10934
                     /locus_tag="ACIE68_003705"
     CDS             10377..10934
                     /locus_tag="ACIE68_003705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909959.1"
                     /translation="MIRDWLDYQNIETAKSAARAAWESLEWTKTSAIASWVSVVLSAI
                     TLGVAIYAMGTWKSQEKVKAKREIKKAAGKLYVEVGLMPKTFTSKNVTNGQVVSKSPN
                     FEVLASQKNQDYIDEWLMHEKLSELYLQLKYLGYAVVDDLTSAQKDALNDLDTHFQNY
                     YSYTESKVNFEKSLKDFLDKMDIYK"
     gene            11705..>11943
                     /locus_tag="ACIE68_003706"
     CDS             11705..>11943
                     /locus_tag="ACIE68_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFX5909960.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPG"
BASE COUNT         3045 a         2674 c         3181 g         3043 t
ORIGIN      
        1 ttttttcacc tttagttcta tagtctaaac gaagaactaa agccggttgt atttaattga
       61 ttcgtgtatc aatgctttgc ccataacata aagttgatct tgggacttct catcaatgta
      121 ccatttctca taggctggat tatccgaaag aacggcgagt ttgttgcctt gcatttgtag
      181 acgtttaacg tggaacgtct taccataaac gaaagagtaa acaccatcag tttggaagtt
      241 gcgaacagaa atgtcgacaa acagtcgatc tccggaaact agagtagggg acatgctgtc
      301 gccatttaca gtcataactt taatatcatc ttgagaacga ttaccgaaaa gagaacgggc
      361 atgctctgtt gtgaactcaa tggcgtagag cacatcaaca tagtctgaaa gcatataggt
      421 tccaggtcct gcgctaacgc taagatccaa aacttctatc ctgtatacat cgggttttgt
      481 tggattgggg atgcttgcca tttccttaca tccttctctc tcgccaacac catattctaa
      541 atatgaagct gataccccca gagccaatgc aagtttattc atgacagagg cacgaggctt
      601 cgcagcgccg attgtgtatc gccgcgccat ttcatatgta acgcccacaa gacttttgag
      661 ttgggtgaca gaaattccct tgtttgtcat tagctcgttt agtctcttgg cgaaatctgg
      721 atacttctgt tcttctacca taggtagaag gttactcgca tcacatacgc tagtcatttc
      781 tattttaagt agttgcattc tgctatttta agtagcatca tccctctgaa tttcagagga
      841 gaaaggtatg tcatctcaaa actacacaga gaaagcagta aaagctgcgg gaaaatcttt
      901 atctgaagta gcccgtcact ttggttttaa gtccactcaa tccgtcgcta attgggtaat
      961 taacaatcaa gtcccgtcag aacgggtttt acaactttgt gagttgggaa actggtccgt
     1021 gacccctcat gaactgcgtc ctgatattta ccccaatcca aatgatggat tacctgagtg
     1081 ctattcaaaa gttagcggtt cagctgcgta aacgtaacca cagaaacgag gaattaaccg
     1141 tgggtaaaga acctgaatgg aaagttgata agcaaccagc atggctggtg gcagcaatac
     1201 gaagaacgat tgctgattta cctcatggct atgaggaagc agcggaaatt cttggtttgt
     1261 ataaatctga tgatatcacc ccagcgaaag atcaattgca taacagactg cgtagcggtg
     1321 gggatcaaat ttttccactt gagtgggcca tggttttaca ggatgccagt ggtaccaagc
     1381 atgtaacgga tgcgatagcg cgtcgtagta atggggtgtt tgtgccgctg gtggacattg
     1441 atgacattga caatggtgac attaatcagc ggctgatgga gtcaatagaa tggattggca
     1501 agcattccca gtacttacgc aaggcaactg ctgatggagt tattgaccgg gctgagcgtg
     1561 agcaaatcga agagaacagc taccaagtaa tggcgaagtg gcaggagcat ttaacactgt
     1621 tatttcgtgt tttttgtgcg ccggaaaaga gtgacgcccg cgagtgtgca gctccgggcg
     1681 tcgtggcgtt ttgtgtccgt ggagaaacta acgcatgaac agtttaacgg caaataaccg
     1741 tttgtcgcaa cagctggtgg tcagtgtcgc tgcacacctg ttgttacggc atgaatgcag
     1801 attaccaaat cacctggctg taagtaacca cagagaactt tacctgactg tggggggcga
     1861 gttgtgcagg aacttaaccg ctggtttcgt gacggaagag gactttatgt tcatgttatt
     1921 cgttgggagc cagaaacaca gcgcgttatc tatcttcgca aagactaccc gcatgagtgc
     1981 tttagtcctt tgtggaaatt caggcgtgat tttgttgagt gtgaaggacc accagcacat
     2041 tgattctgcc attccgggac gttacactgt tcaggcacct tataaagcgg gtgccgggat
     2101 tggcgtcctg aaattgatta ctgagcataa ccgcgctcat gcggtttttt cgtgtcatga
     2161 gcattgctac gcccaaatta tggtggggcg tacagggccg acttcggtcg ggccgggttc
     2221 ggtagtctcc ggtaacgcca accctgtacg tctcaccacc tctgtgattg gcgtcccatg
     2281 tggtgagttt tctaaaaaac tgactaccgg ggctgtcacc atgactactc tcccaaccct
     2341 ctctcaacct gaaattgcca tcgttgatgg tcaggctgtt acttcttctt tggctgttgc
     2401 tgacttcttc tctaaacgtc atgacgatgt tctgaaaaag atccgcattt tggattgttc
     2461 tccagagttt tgtgcccgca attttgcgga gacatcaatt ttggtacgcc agcccaacgg
     2521 cggtactcgc aaacttcctt gctatcaaat aacccgcgac ggctttgcgt ttcttgctat
     2581 ggggttcacg gggaaacgtg ctgcccagtt caaagaggca tacatcaatg cctttaacca
     2641 gatggagaaa cagctttcaa agccctctgt accgagcgac gttgcacata acgccagcgt
     2701 tctctattcc tacatttcat caattcatca ggtctggttg cagcagcttt atcccatgct
     2761 ggaaaaagct gaatcaccgc tggctgtaag tctgtatgac cgaattaacg atgcggcatt
     2821 tcttgcccgt cttattcatt cgtcgctgaa ctcttcagag gtaagggggc gcaaatgatc
     2881 cggaatattt tcaaacgatt taccaatcag actttccgtt gtcctcgccc gggtcagtgg
     2941 tacaccacgc ctgcagggca tgttctacgt gttagcctgg tggaccgtga atgtcagaag
     3001 gtgatttgtg aaccgctggg ccgtaattac cgcgtcagta tgccgcttat agccttttgc
     3061 tccggaaaaa acatgaagca tctcggaggt gcagcatgag tatggagctg atggttaaag
     3121 cgatgaaaat tcgagtgggt aatccattgc gaaaactggt tctgatcaag ctggctgata
     3181 atgccagcga tcagggtgag tgctggccca gctaccagca tattgctgac cagtgcgaga
     3241 ttagcaaacg ttctgtgatg aatcatattg cggccctttg tgattccggg ctggtaaaaa
     3301 aagtcacccg gaaaggtgaa aaaggtaact caagtaatat ctatctcctt catcttgatg
     3361 gtgcaggaga ttcactaggg ggtagtgcaa ataattcact atctggtgca gcaaattcac
     3421 caggtagtgc aggagttgca ccagggggta gtgcaggaga ttcacccaga accagtcact
     3481 cttttgaacc agtcaaagaa ccagtcaatg aaccaatagc tgttggtgca tctgctgatg
     3541 agtctgtgcg agttcgttca aaccgaccgg aatactctcc ggagtttgag caggcatggc
     3601 tggcataccc caaacgtgct ggtggcaatt caaaatctgc agccttcaaa gcctggaaag
     3661 cccgtttgaa tgagggggta aaacccgaaa ccatgctgga aggtgtgaaa cgctacgcgg
     3721 gctgggtatc tgcgatgggt aacagcggaa cacaatttgt gaaacaggct gtcacgttct
     3781 ttggtccgga tcgtcatttc gaagaatcct gggaagttcc tgcggtatct gcagccagac
     3841 gcgaggaccc gtacttcaaa gccagttacg acaacgtgga ctacagccag atcccggcag
     3901 gattcagggg gtgatcatga gtcttttgaa tgaagttcag aaattcattg aagcccatcc
     3961 gtggtgtact tccggagaca ttgcggatgc ttttgcaggt tactcacggc agcgcgttct
     4021 gcagtcagca agcaagttac gtcagagtgg gcgtgtggct caccgttgtg aaggagatac
     4081 acgcagacat ttcccacgcc tgactgagag agcgcaggag ccggaaccac aaccagttcg
     4141 tgaaaccaga cctgtgcgca atttctatgt cggcactaac gatccccggg tgattttgtg
     4201 cctgacccgc caggcggaag aactggagtc taggggctta taccgtcgtg ctgcaacggt
     4261 gtggatggcg gcattccgtg aaagccactc ccagccagaa cgaaacaatt ttctggcgcg
     4321 tcgtgagcgg tgcttacgga aaagcagcaa gcgcgctgca tcaggtgaag agtggtatct
     4381 gtcagggaat tacgtggggg cttaatgagt aataaatatt gccaggcgct ggtggagctg
     4441 cggaacaaac cagcccatga actgaaggaa gtgggcgatc agtggcgcac gccggataac
     4501 attttctggg gaattaacac cctgtttggc ccgtttgttc tggatctgtt cactgacggt
     4561 gataacgcca aatgtgccgc gtattacacg gcggaagaca acgcgctggc gcatgactgg
     4621 tcagaacgtc ttgcggagct taaaggtgct gcctttggta atcccccata cagccgcgcc
     4681 agtcagcatg aggggcaata catcaccggc atgcgttaca tcatgaagca tgccagtgcc
     4741 atgcgtgata aaggcgggcg ctatgttttc ctgatcaaag ctgccaccag cgaagtgtgg
     4801 tggccggaag acgcagacca tattgctttt attcgcgggc gtattggttt tgaacttcct
     4861 gcctggttta tcccgaagga tgagaagcag gtgccgacag gcgctttctt cgctggtgct
     4921 attgctgttt tcgacaagac ctggaaggga ccggcaatca gctacatcgg gcgcgatgaa
     4981 cttgaggcat gtggcgaggc gtttctggcg caggttcgcc agcaggcgga aaaactggtc
     5041 agggagatgg cggcatgacg acgttaactc aatgccagca gcaggtgctg gatatgctga
     5101 tttcttacca gcaagaacgt ggcttcccgc caaccaatca ggaggtggca accatgctgg
     5161 gataccgttc agtgaatgca gcggtggagc atcttcgcgc actggagaaa aaaggcgtca
     5221 tcacgataaa gcgtggcgtg gcccggggga taacgcttca taccgcggtg aaggacgacg
     5281 acagcgaggc ggtcgggatt atccgcgcac tgcttgccgg tgaggaaaac gccaggctgc
     5341 gtgcagccca ctggttacat gagagggagc tgaaagtatg aagctgattc tgccttttcc
     5401 gcccagcgtg aacacgtact ggcgacaccc caacaaaggg gcgtttgctg gtaagagcct
     5461 gataagcgcg gcggggcgaa aattccagag cgcggcgtgt gcagcaatag ttgagcagtt
     5521 acgtcgtctg ccaaaaccaa cgtcggcacc tgcttcagtg gagatcgtgt tgtttcctcc
     5581 ggataaccgg atccgcgatc tggacaacta taacaaggcg ctgtttgacg ccctgaccca
     5641 cgcgggtgtg tgggaagacg acagtcaggt gaaaagaatg ctggtggagt ggggaccggt
     5701 tatcccggaa gggaaggtcg agatcactat cagtaagtac gagaaaacgg tgggtgcagc
     5761 cgcctgatta agaggagaaa cgaagtatga ataatctgat ggttattgat ggtattgaag
     5821 ttcgtcgtga tgcttatgga cgttacagcc tgaacgatct gcatcgcgca gcagtagcat
     5881 ctggtgcaaa tgccagaacc aaggagccag gaaagtttct ttccagccaa caaactgttg
     5941 agcttgttca tgaattgacc aacacccaga atttgggtgt tgacccggtg agtgtgattc
     6001 atgggggaaa tgaacgggga acgtatgtct gtaaggaact ggtgtatgcc tatgcaatgt
     6061 ggatcagccc gtcattccat ctgaaggtga tccgtacttt cgatatggta accagcgcgc
     6121 cggaaaagtt atccggacag gctgctgaca agatgcaggc tggcgtgatc ctgctggact
     6181 ttatgcgccg ggagttaaat ctgtctaact cttcagtgct tggtgcctgt cagaaactcc
     6241 aggaggctgt tggcttaccg aatctggcac cgcgctatgc cattgatgct cctgctgacg
     6301 cgcctgatgg ctcaagccgc cccacgctgt cactgagtgc actgctgaag cagtatggta
     6361 tccgcctgac agctaatcag gcatatcacc agatggtgaa gctggggatc gtcgagcagc
     6421 gcgaacgata cagccgtacc gcgattaaca acatcaaaaa attctggtcg ctgacagcga
     6481 aaggctgcat gttcggcaag aacatcacca gtcccgcaaa tccgcgcgag acgcagccgc
     6541 actttttcga atcccgattc cctgagctgt taaagctgct cgataccgtt cattgaggtg
     6601 accgtgagag cactactgac ccctgaaatt gccccgcgta tggggatcgt attgttcaga
     6661 cccggttcag agctgatgcc cctgtttatg caggggcgtg tcctgctgga gcctgagccg
     6721 gaacgttatt catcttttgc cagtggtgcc gttccggcgg catcacaacc gctggcggat
     6781 gatcctgccg ttcgggccgt gttccgcaat gaggcagtga tccgtcgtgc tggtggagtg
     6841 gaatgtcttg aaagctggtt acttcgtgaa aaaggctgcc aatggcctca ttccgactgg
     6901 cacagcgaga acatgaccac aatgcgacac gcgccgggcg caatccgtct gtgctggcac
     6961 tgcgataacc agctgcgcga tcagttcacg gaacggctgg aatcaatggc aacggataac
     7021 tgtgcccgct gggtgttgtc tgtcgtgcgt cgggatctcg gttttgatga tagtcacgtt
     7081 gtgacaatgc cggaactgtg ctggtggctg gttcgtaatg acctggcgga tgccttaccg
     7141 gaaagtgcag cccgtaaggc actgagatta ccgaagcctg ttgtgccgtc tgtcacccgg
     7201 gaaagtgacc ttgtgccttc ggttcctgcc accagcatca tccaggataa agcgaaaaag
     7261 gtgctggcgc tgaaagtgga tccggagtcg ccggagtctt ttatgttacg cccaaaacgt
     7321 cgccgctggg ttaacgaaaa gtacacgcgc tgggttaaga cgcagccgtg tgcatgttgt
     7381 ggaaagcctg ctgatgatcc ccaccacctg ataggccacg gtcagggcgg gatgggtaca
     7441 aaagcgcatg acctctttgt gttgcctttg tgcagaaagc atcacgacga gctgcatgcg
     7501 gataccgtag catttgaaga gaagtatggt tcccagctgg agctgatatt tcgttttatc
     7561 gatcgtgcgc tggcaatagg cgtactggcg taagtggaga acgagcatga accttgaagc
     7621 cttaccgaaa tattactccc cgaaatctcc aaaactgagc gatgacgcac cggcgacagg
     7681 ctctggtggt ttaacaatta cggatgtgat ggctgcgcag gggatggtgc agtcgaaagc
     7741 accgcttggg tttgccttat tcctagcaaa agttggtgtt caggatcctc agtttgcgat
     7801 tgaaggtctg ctcaattacg cgatggcact ggataacccg acattgaata aattgagtga
     7861 agaaacccgg ctacagatca ttccttacct tgtgaatttt gcctttgctg attattccag
     7921 gtctgcggca agtaaggctc gctgtgagca ttgtgctggt actggatttc ataatgtatt
     7981 gcgcgaagtg gtgaaacact ccagaagcgg ggaatctgtt atcaaggaag agtgggtgaa
     8041 ggaactatgt cagcattgtt atggtaaggg agaagtcagc acagcgtgca gagggtgtaa
     8101 gggtaaaggt attgtcctgg atgaaaaaag aacccggctt catggtacgc ctgtttataa
     8161 gatttgtggg cgttgcaatg gtaaccggtt tagccgttta ccaaccacac tggcgcggca
     8221 tcatgtccag aaactggtac cagacttgac ggattatcag tggtacaaag gatatgcaga
     8281 tgtcattgat aaactggtta caaagtgctg gcaggaagaa gcatatgcag agacacaatt
     8341 gagaaaggtg acaagataaa tgatgttcga cgaagatggc gacatgatgc ttgcattttt
     8401 caaaaaatct ggttagaatt ctcctaacga tgggctttgt gtgtctatcg ttgataatct
     8461 tcaagaacct gccaccgagc gggtttttta ttgatgtcaa ttgtgttttt aaggctcacc
     8521 ttccttaaag tgtgttgtac aaaaaactgg cagccagcta cgctcatttt gaaaaagtga
     8581 cacccttcaa tgtttctttt gaatggaatt gctacccata aatctctatc aaaaacagga
     8641 gagcatatat ggtggagcgt tgttctgttt gtgagcagtc attaaactat tcacgagaag
     8701 ttgaacaaga aggggttgaa tataaatctt gcccaaactg ttctgctgat gccggagtgc
     8761 acgtttttta taaaacaata gactttggtt atagggatat gggagacggc aggcatatcg
     8821 ttcagtcatg gtgtccggct tgccgttctg gtgaaaaacc ttctatacca ccagcattta
     8881 aatgttgtta actcaatgaa ttataaaaag aggctgcctg tgggcggcct tttttgtgca
     8941 ctacgcatct tttgcgactc agcgctataa ccagcttctt tcccttcact cgttgcactt
     9001 ccgataaccg gaggtgggaa ttatgaaaat gcataacgat cctcattcct ggtctgactt
     9061 acttgaattg ttacagagct ggtggcatgg agacacaccg ctgggcgcag taattatgtc
     9121 gatcgttatg gctggcttgc gcattgccta ttttgacggt ggtggtggct ggaagcgaaa
     9181 aacgctcgag attttgctct gtggcgctct gacgctgact tttgcatccg ctcttgagta
     9241 tgtcggatgg cctaaatcgc tttctgttgc cattggtggc ggcgttgggc tgatcggggt
     9301 cgatgcaatt cgtggggctg caatgagagt aatcggtaat aagtttggtg gctctaagga
     9361 gtaatttatg caggtactaa attcccagcg taaagctttc ctcgatatgg tggcctggtc
     9421 agaaggaaca gataacgggc gacaaccgac acgtaaccac ggttatgatg ttattgttgg
     9481 cggcggactg tttactgatt actccgatca ccctcgcaaa cttgtcacgc taaaccccaa
     9541 actcaaatca acagccgccg gacgttacca gcttctttcc cgttggtggg atgcttaccg
     9601 taagcagctt ggcctgaaag acttctctcc caaaagtcag gacgctgtgg cattgcagca
     9661 gattaaagag cgtggcgctt tgccgatgat tgaccgcggt gatattcgtc aggcaatcga
     9721 ccgttgcagc aatatctggg cttcactgcc gggcgctggt tatggtcagt tcgagcataa
     9781 ggctgacaac ctgattgcaa aattcaaaga agctggcgga acggtcagag agattgaggt
     9841 atgagcagag taaccgcgat tatctccgct ctgattatct gcatcatcgt ctgcctgtca
     9901 tggtctgtta atcattaccg tgataacgcc attacctaca aagcccagcg cgacaaaaat
     9961 gccagagaac tgaagctggc gaacgcggca attactgaca tgcagatgcg tcagcgtgat
    10021 gttgctgcgc tcgatgcaaa atacacgaag gagttagctg atgcgaaagc tgaaaatgat
    10081 gctctgcgtg atgatgttgc cgctggtcgt cgtcggttgc acatcaaagc agtctgtcag
    10141 tcagtgcgtg aagccaccac cgcctccggt gtgggtaatg cagcctcctc ccgacttact
    10201 gactccgctg aacgggatta tttcaccctc agagaacgag tcgacacagt gacgaagcag
    10261 gttggttatc tgcaggaata tgtgaaaaga caatgcgttc agtgacaaaa aaaattacaa
    10321 tccgtatcaa agatgaataa aatctgaact cataaacaaa tgttggatac atatcaatga
    10381 tacgggattg gttagattac caaaatatcg agactgctaa gtcagcagcg agggccgcgt
    10441 gggagtcttt agaatggacg aaaacttcag cgattgcctc ttgggtttct gtagtgctct
    10501 ctgcaataac cttaggcgta gctatctacg caatgggcac ttggaaatca caagagaaag
    10561 taaaagctaa acgtgagatc aaaaaagcag caggtaagct gtatgtagaa gtagggctaa
    10621 tgccaaaaac attcacaagc aagaatgtta ctaacggtca ggttgtttcc aagtcaccta
    10681 attttgaagt attggctagc caaaaaaatc aagactatat tgatgaatgg ttaatgcacg
    10741 aaaagttatc ggaactgtat ctacagctca aatacttggg gtatgccgta gttgatgatc
    10801 tgactagcgc ccaaaaagat gcgctaaacg atttggatac acattttcaa aattattatt
    10861 cttacaccga aagtaaggtg aattttgaaa aatctttaaa ggattttttg gataagatgg
    10921 acatctataa atagtcagat cctatcggta agccgcctcc gggcggtttt ttgttgccat
    10981 cacaatgggc aggtccatcg taacggcagt atcttatata caccaatgac atctctggat
    11041 ggttggcagt cctgttagtt ggcctgcggt ggactcggcc tcgtcaagcg taagaacaca
    11101 tactttttgg tatcggccag tagtaaagct ggcgtgattt ttaggactga ttaacgggtt
    11161 gattgatttg catgaggctc gatgaatccg cgtaaatctg gcatcacttg ttctgccttt
    11221 cccgctggcc gacttaagca gattaataac gaatccatcg gaatgggtcg ctagccagct
    11281 cttgtacatc gcttcgctat caacatcctg tatttcactg ctgaaaatag taatgcccat
    11341 attttttccg tatatgacta actgatatga atatgtatca ggactatttt atggcaaaac
    11401 cagagtagaa ggccattggg tccggagtgt tgatgtgaac aaattacccc ccctttaagt
    11461 gtaattgata tgtattatca ttttcgggtc cttttcggcg atccggagcg ttacggggcg
    11521 gcgacctcgc gggttttcgc tatttatgaa aattttccgg tttaaggcat ttccgttctt
    11581 cttcgtcgta acttaatgtt tttatttaaa ataccccctg aaaagaaagg aaacgacagg
    11641 tgctgaaaac gagcttttgg gcctctgtcg tttcctttct ctgtttttgg ccgtggaatg
    11701 aacaatggaa gtcaacaaaa agcagctggc tgacattttc ggtgcgagta tccgtaccat
    11761 tcagaactgg caggaacagg gaatgcccgt tctgcgaggc ggtggcaagg gtaatgaggt
    11821 gctttatgac tctgccgccg ttataagatg gtatgccgaa agggatgctg aaattgagaa
    11881 cgaaaagctg cgccgggaag ttgaagaact gcggcaggcc agcgagacag atctccagcc
    11941 agg
//
LOCUS       DBANUU010000130        11921 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000130 DBANUU010000000
VERSION     DBANUU010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.131"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            118..720
                     /gene="ais"
                     /locus_tag="ACIE68_003707"
     CDS             118..720
                     /gene="ais"
                     /locus_tag="ACIE68_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFX5909961.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(759..1184)
                     /gene="nudI"
                     /locus_tag="ACIE68_003708"
     CDS             complement(759..1184)
                     /gene="nudI"
                     /locus_tag="ACIE68_003708"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFX5909962.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1463..2005
                     /gene="yfaZ"
                     /locus_tag="ACIE68_003709"
     CDS             1463..2005
                     /gene="yfaZ"
                     /locus_tag="ACIE68_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFX5909963.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2105..3307
                     /gene="yfaY"
                     /locus_tag="ACIE68_003710"
     CDS             2105..3307
                     /gene="yfaY"
                     /locus_tag="ACIE68_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFX5909964.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            3371..3832
                     /locus_tag="ACIE68_003711"
                     /pseudo
     CDS             3371..3832
                     /locus_tag="ACIE68_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(3873..4775)
                     /gene="rpnE"
                     /locus_tag="ACIE68_003712"
     CDS             complement(3873..4775)
                     /gene="rpnE"
                     /locus_tag="ACIE68_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFX5909965.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRTYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(4969..6159)
                     /gene="glpC"
                     /locus_tag="ACIE68_003713"
     CDS             complement(4969..6159)
                     /gene="glpC"
                     /locus_tag="ACIE68_003713"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFX5909966.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(6156..7415)
                     /gene="glpB"
                     /locus_tag="ACIE68_003714"
     CDS             complement(6156..7415)
                     /gene="glpB"
                     /locus_tag="ACIE68_003714"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFX5909967.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(7405..9033)
                     /gene="glpA"
                     /locus_tag="ACIE68_003715"
     CDS             complement(7405..9033)
                     /gene="glpA"
                     /locus_tag="ACIE68_003715"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFX5909968.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9306..10664
                     /gene="glpT"
                     /locus_tag="ACIE68_003716"
     CDS             9306..10664
                     /gene="glpT"
                     /locus_tag="ACIE68_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFX5909969.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            10669..11745
                     /gene="glpQ"
                     /locus_tag="ACIE68_003717"
     CDS             10669..11745
                     /gene="glpQ"
                     /locus_tag="ACIE68_003717"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFX5909970.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
BASE COUNT         2796 a         2920 c         3192 g         3013 t
ORIGIN      
        1 ggcattttgc cgacatcgta ggatttttaa tattacacca actgctaatt atcgccagaa
       61 atgtcgctca attttacggt aattgtctgg ttgcgcttgt ctataggtgg agtttacgtg
      121 ttagcttttt gccgctcatc attgaagtca aaaaaatatt tcatcatttt actggcgctc
      181 gctgcaattg ccggactggg tactcatgcc gcctggagta gcaatggttt gccacgtatc
      241 gacaataaaa cattggccag actggcacag cagcacccgg ttgtcgtttt gtttcgccat
      301 gctgaacgtt gcgatcgttc aaccaatcaa tgcttgtcag ataaaacagg tattacggtt
      361 aaaggtaccc aggatgcccg tgaactgggc aacgctttta gcgctgacat ccctgatttc
      421 gatctttatt ccagtaatac cgtccggacc attcagtcgg cgacctggtt ttccgcgggt
      481 aaaaagctga cggtagataa acgacttctt cagtgcggta atgagattta tagtgcaatt
      541 aaggacttac aaagcaaagc gcctgataaa aatatcgtta ttttcaccca taatcattgc
      601 ctgacatata ttgctaaaaa taagcgtgat gcgacattta aacctgatta tctggatggt
      661 ttagtcatgc atgtggaaaa aggcaaagtt tatctggatg gggaattcgt taaccactaa
      721 attttcaatc tgacagccag tgatggctgt catcattgtt aaagaagacc tttcaaacgt
      781 aacgtttttc gggtggcaac atttaaatca taatgcacca gatcttcagg ttttacccac
      841 gcgtagtcct gaaactcttc gtttattttc acttctcggt tggcagaaac gcagtcaaaa
      901 atcaggtaaa tcatataaat ctcttccttg cgaccatctg catacgtctt ggtgcgaata
      961 tcatcgctga aggtccacgg cgtgatttct gtcaaaagca gctgttctcc cagttcttcg
     1021 cgaatttcgc ggcgtagtgc ctcttcaatt cgttcgccag actccacgcc gccacccgaa
     1081 agcgcccatt gaccggggaa aacgccgcga tcgtcggcca ttttacacag caaataagca
     1141 ccatcatttt gaatcaaagg gcatacaata gtccgttgtc gcataggggt attccttata
     1201 aataaaaaga aaccatgaga atacggtaac tcagaaatat gtgaaagtga cagtaatctc
     1261 ttttgcaatg ataactgcac ttattattat ttttgtgtgt atcggtaaag tgtgtttatt
     1321 ttttcggcgt ttaattattt aagaagtatt tagttacaat aagtgactag atcccttttt
     1381 tcgctcattg aatcctctgt gccaggcagt acaatcccgc agctttggga attttgatta
     1441 attgcttgtg ggaaagggga taatgaaaaa aattgcgcta gcaggtctgg ccggaatgct
     1501 gttggtttct gcatcggtca atgcaatgag catcagcggc caggcgggta aagaatacac
     1561 caatattggt gtcggttttg ggactgaatc gacgggcctg gctttaagcg gtaactggac
     1621 acataacgac gacgacggtg acgtcgcggg cgtggggctg gggttgaatc tgcctctcgg
     1681 gccgttaatg gcgaccgttg gcggaaaagg cgtttacacc aacccgaatt acggcgatga
     1741 aggttatgcc gcagcggtag gaggtggttt gcagtggaaa attggcaaca gcttccgttt
     1801 gtttggcgag tattactact ctccggattc gctctccagc ggtattcaaa gttatgagga
     1861 agcgaatgct ggcgcgcgtt acaccattat gcgtccagtc agtattgagg cgggttatcg
     1921 ctacctgaat ctgtcgggta aagacggtaa ccgcgacaac gccgtggctg acggcccgta
     1981 tgttggggtt aacgccagtt tctgatccct tccggcgcgg cattctgtcg cgccattcag
     2041 tttcttacta cgccttatct ccctttgcgt tatagtgatt cgataacaaa agcaggagat
     2101 aaccatgtta aaagtggaaa tgttatccac cggggatgaa gtgttacacg ggcaaatcgt
     2161 tgacactaac gctgcctggc tggccgattt tttctttcat caggggctgc cattatctcg
     2221 ccgcaatacg gtgggggata accttgatga cttagtcacc attcttcgcg aacgtagtca
     2281 gcacgccgat gtgctgatcg ttaacggcgg gctgggaccg accagcgatg atttaagcgc
     2341 actcgccgct gcgacagcaa aaggtgaagg cctggtgctg catgaagcct ggctcaaaga
     2401 gatggaacgc tatttccacg aacgtggacg agtaatggca ccgagcaacc gtaaacaagc
     2461 ggagctgcct gccagtgctg aatttatcaa taacccggta ggcaccgcct gtggttttgc
     2521 cgtgcagctt aatcgttgcc tgatgttctt tactcccggc gtaccgtcag aatttaaggt
     2581 gatggtcgag cacgaaatcc tgccgcgcct gcgcgagcgt ttttctttac cacagccgcc
     2641 ggtttgtctg cgtttaacta cctttggtcg ttcggaaagc gatctggcac aaagcctgga
     2701 cactctacaa ctgccgccgg gcgtaacaat gggctatcgc tcctcaatgc cgatcatcga
     2761 actgaaactc accggaccgg caagcgagca acaggcgatg gaaaaactgt ggctggacgt
     2821 taaacgtgtt gccggacaga gcgtgatttt cgaaggcacc gaaggactgc ccgcgcagat
     2881 cagtcgcgaa ttgcaaaacc gccagttcag cctgacgttg agcgagcaat tcaccggtgg
     2941 tttattggct ttgcaacttt ctcgcgcagg tgctccattg ctggcgtgtg aagtggttcc
     3001 ttcacaggaa gaaaccctgg cgcaaactgc gcactggatt acagaacggc gggccaacca
     3061 ttttgccggg ctggcactgg ctgtttcggg tttcgagaac gagcatctca actttgcgct
     3121 agccacgcca gacggcactt tcgctctgcg tgtgcgtttc agcactacgc gctacagcct
     3181 ggctatccgt caggaagtgt gcgcaatgat ggcactgaat atgctgcgcc gttggttaaa
     3241 cggccaggat atcgccagtg agcatggctg gattgaggtt attgagtcca tgaccttatc
     3301 tgtctgattc cgccactctg tgccggttta ctgcaccggc acattccccc tccggcaaaa
     3361 cttgtccaac cctccctacg gtgagcgtgg tgttggggcc agtgtggcac gggctgcgcg
     3421 ctggggacgc attgagaatt acatggcgca agtcaacgat tcgctttgtc tgttagtgca
     3481 ggtggaaagt aaaacggcac tggataacct ggacgagatc ctcgacgtcg aagggattga
     3541 tggcgtgttt attggaccag cggatctttc tgcgtcgttg ggctacccgg ataacgccgg
     3601 gcacccggaa gtgcagcgaa ttattgaaac cagcattcgg cggatccgtg ctgcgggtaa
     3661 agcggctggt tttctggctg tggctcctga tatggcgcag caatgcctgg cgtggggagc
     3721 gaactttgtc gctgttggcg ttgacacgat gctctacagc gatgccctgg atcaacgact
     3781 ggcgatgttt aaatcaggca aaaatgggcc acgcataaaa ggtagttatt gatatcaaag
     3841 gcccatgggg atcggctgtg ggcctgtgtt aattagtggt tattcgctgc cagatcggct
     3901 tcgcttagct gggtggccgc gagcacctgg tcacgatcaa tgccctgttc cagcatgcgc
     3961 aaagcaattt taatggcttg ttcatgcata ccttcctgcc agccaatttg atgcccttcc
     4021 tgccgtagcc gttcagcaat agtcattaat ctctcctttt gtagtggtga acgttcggca
     4081 atctcctcaa taaaacgggt gaaacgggag gtatcgccgc attgcagcag ataattaaac
     4141 agtgtttgta gctggctgtc attagtgaag cctctaacca aaagcgtggt gatcctgtcg
     4201 accatgccga ttaaatcgcg gtcgcgaata tgcttttgaa tcagttccag cagagctata
     4261 cgccgatgtt gcatgatctc atcgtcaggt acgatggtga tatccaccaa cggaaaagct
     4321 tcggtgtaca actgccgggc aagttgcgga tcgtcaaact catccagcca gttaagtgag
     4381 tacgggtagg gcgatgtttc gccatgataa aacagcaatg gcaccaccag cggaactctg
     4441 tcatagcctt tatccaggtg acgctgcatg gcggcagtgg catagcgcat tagccgaaaa
     4501 gccatattct tttctgcaga gctttgatgt tcaatcacgc agtagatata gccgtcaccg
     4561 tcgctggttt ccacggacca caaaacatcc gagtagtaag tgcgtaaact tttttcaata
     4621 aaactagtgg gttccaggcg taaggtttgc aggttgcaaa gcttgcgcag tggttctggt
     4681 aaatgtattt cgagaaaatc ccgtgcggtt tcgggtgtga acataaaggt tttaaacacc
     4741 gcatcatgcg gggaggatgt tgttgattcg gtcatggttg tccgtcacct gatatttatg
     4801 atgacgcgac attacgtcta tcccgggcct ttgacatctg tcagtttcag cctttgcgag
     4861 acattttcag aggaattttc aaagcgttgc aacgcataca tggtgcgtgg aatcgcgctc
     4921 gcaaaaatgc ccgataagca atgtgcttac cgggcatcaa aaggtcggtt aagccagcgc
     4981 ctgggccagt agcgtaatcg gatgttcgca gcgaaggctc gtggacatct caatctgcca
     5041 tttgcaggtt tcgcagtcgg tgatgaccag atccgcgccg ctctcttcta tctggcggaa
     5101 cagtggcgca ccgatggctt gtgaggtggg gtagttctct tttttgaaac cgtaagtacc
     5161 cgcaataccg cagcactggg aatccagcac cgttaactca agccccggga ttttacgcaa
     5221 cagctccagg gtgtagagcg tccagcccat tttttccata tggcacggag tgtgataaac
     5281 cactttcagc ggcagcggtt tcagcggtaa cgttttgcct tcgtccagct tgcgccacag
     5341 ccagcgggtt gccagttcga tatgatcgcg caagcctttg ttgtcgacat tcagcacttc
     5401 cgggtattcg tcgcgcaggg caaatgtaca ggttgaggag gtggcaatca ccggaatgcc
     5461 ttttactccc acagcttcgc ggatcgactc tacattcgta attgcctgtt tgcgtgcttt
     5521 atcggtaaag ccgttggcga ttagcggtac gccgcagcat ttttctttgc tgagcagttg
     5581 tacaccggta cccattgcgt tgagcacttt aattaaatct ttacctaact gcggatggtt
     5641 gtagttaacg aagcagccgt gaaagaaagc gacctggtct ttatattgtg cttgctgagc
     5701 cgctatgctg cgataccagc gacgaaacgt accgaaggag tatttcggta gcgtgcggcg
     5761 atgatcgatt tttaacgccg catcaagcag ctgccgcacc ggtttcagcg aggtagcggt
     5821 gttgacgatt ggtgcgaacg gcgtggaaac gctacccatc aggtcggtat gactcaacac
     5881 aaaattacgc agcgacgggc gcgtggtgtc atattttgcc cgcgcgcgct ggataatatc
     5941 gccaatcttc acatcggacg ggcaggcgac ttcacaacgt ttgcagttga tgcaatattt
     6001 cagcgcctcg tcatacagtg cgccatcttt caaacgcaga cgctcgccat ccggcccggc
     6061 ttgttttggc cctggataac cgggattcac ccggctcacc gggcaggcgg tggtgcagac
     6121 ggtgcactta atgcagtttt cgaagctggt gtcgttcatt gttggcctcc tgcgcgttgg
     6181 gcaatctgtt gagcggcatg taaagcactg acggcacaaa caccgccgcc gcatccctgg
     6241 gcgatgggat caaatccgcc cagcaccgaa ccgatggcaa acaggttctc aatggtttgc
     6301 cctgcctgcg acgggcgtag cgtctgatca gtggttacac cgaactgctg ccacggttgc
     6361 ggcgcaaaaa aatcgccctt ataccattca ccccgcgtgg cggtttgtag cacatcaagg
     6421 ccgagaatcg gctcgcgaat gccgttacgt tccgctacca gtccgccact aaagaaactg
     6481 ccgctggcga gaaccgcgaa acgtggacgt agcggaatat cggcgtgatt gcgggtccag
     6541 atttcattca ctacgccatt tttacaggtc acttttttca cttcatcgcc cggcatccac
     6601 acgccaccct ggcgcacaaa ctggcgctgt aactggtttt gcagacgaat gcccagcacg
     6661 gaaggcggca gcgttggcaa aagcatcagt gaacaaggta gtttttcatt caaccaacgc
     6721 cacagtttgt catcggccag gccgaagcag gcgggcatca ggatcatttc gcaggtattg
     6781 gcgacaggaa taagcgcatc aagtaacagc ggccagtttt cttcattatc aaggaaacgg
     6841 gcgatattca ccgcgcgaaa ttcggtggcg ttattgcgca ggacatccag ttccggcagc
     6901 tctatttctg cggtttcaac cgaaagatcc agttcgcgca acgaagccgc cgcaagatgc
     6961 gcctggaaat ccatcagccc gcttatcccc actacacata tcttcttcgc gggtagcggc
     7021 cagacgggaa cttctggcga acttaaccag gttgcacgca aagtgccgag cggggtaacg
     7081 cgctgatggg ccagttccac gctgccctgc aattgcgcac cgctttcagc gatcaatgcc
     7141 tgcgcctggc aagcgagatc gagcacgcgt tgtggttcga gaagactgta aggatgggct
     7201 ggtgcctgct gacgcaaaga ttccagtcca ctgtgaatgt ctgtcaccgg ttgaccatca
     7261 ggcagatggc tcagcaaatc cagcgaaccg gatgagaaat gcagcgcgct ttgaccacgc
     7321 gtgacaatgg cacagcgcag gccgtgtttt tgcagttgca ggccacagag taatccggcg
     7381 aggccgccgc ccataatgac agtatcaaag cgcatctttc tgctccttct ccagaccaca
     7441 caatccctga taaacccagc gggtaaattc gctttcgcgc agtgcatctc cccaggcgat
     7501 gggttgcacg cctttccagc gttcgttaag gaaggtggaa agttgctcga tagattgcgc
     7561 ggacgtcgtg acgttaaaac gttgcagcag tccggcagcg cggcaggcgc agagttcgcc
     7621 ctggcaggta cccatcccca cacgggtacg acggcgtaaa tccagcaggc tattaacgtt
     7681 taaattttct accgcgtact gaacttcacc cgcagttacc gcttcgcact cacataccag
     7741 gctacgatgc agacggcctt cgctcagcca ggcaggcgtg cgatcgccat gacgataaac
     7801 cgcagaaccg cgcagcgggg caggcaggga gatgacttta cgcaaggtaa cttcagcggg
     7861 ttcttgtgaa ccaggcagtg ccagatcggc agtcgtacag gggcgcgtgt tgcccagttt
     7921 gcggcatacc gcgtcggtag cccattcagc catcagccga taggtcatca gtttgccacc
     7981 ggtgatggtg ataaatccgt ccagaccatc gcgttcagca tggtcgagca gcacgatgcc
     8041 acggctgacg ttacgtccgc tcgggtcgtc atcgctggca accagtgggc gcacgccaga
     8101 ataggcccgc aaaatgcgcg ttttcgccat cacgggggcc agtttttccc cttcacgcag
     8161 cagaatatca acctcttctg ccgtcactcg attatcgtca atctcgttgt aatcaatacg
     8221 taaagaggtg gtgccaatca gcgaaatggt atcgccaggc accagaatat cggcgtcgga
     8281 aggtttacgg cagcggttga tcacatgctg gttaatgcgg tgatccatga tcagcagcga
     8341 tcctttcgcc gggaacatgc gaatgcgcag atcggcatat tcggcaatgt gttgccccca
     8401 gatcccagcg gcattaacca cgacaggtgc atgaagggcc tgagtttcgc cggtgagatg
     8461 gttacgtaca cgaacaccgc acaccgtcgc gccttcacga atcagccccg tgacttcatg
     8521 agcggtaagg ataacggcac cgtgttcttt ggcatccagc atgtttgctg cggtcagacg
     8581 aaatggatca acggtgccat ccggaacttt caccgcgcca atcagtgccg ggttaacggc
     8641 aggttcgata atgcgcgctt gctgcgggtc tatagcttct gcgctgatcc ctgcttcttc
     8701 gcaggcgcga ataaaagtgg cctggaatga gaggtcatct tccggcaggg tgataaatag
     8761 gccgttggtt ggttcaacgc agtgacgtgc aatgcgtttc aggatctggt tttcactaat
     8821 gcattcgcgg gccgattccg catcggttac cgcatagcgc gcaccgctgt gcagcaggcc
     8881 gtggttacgc ccggtggcac cggttgcgat gtcgtggcgc tcaaccaaaa tcacgcgcag
     8941 cccgcgcagg gcacagtcgc gggcaatccc ggctcccgtt gcgccgccac caatgataat
     9001 cacgtcactt gattgcgagt cgcgagtttt cattgttatc cctctgaagt tcgtttttta
     9061 ccatttagcc atagtaaaaa catgaattgt ttgatttcgc gcatattcgc tcataattcg
     9121 aaagtgaaac gtgatttcat gcgtcattct gaacattttg taaatcttat ttaataatgt
     9181 gtgcggcaat tcacatttaa tttctgaatg ttttcttaac atcgcggcaa ctaaagaaac
     9241 ggcaggttct cccactgaat cagtctgtta atcataaata agaccacggg ccacggaggc
     9301 tttcaatgtt gagtattttt aaaccagcgc cacacaaagc gcgcttacct gctgcggaga
     9361 tcgatccgac ttatcgtcga ttgcgctggc aaattttcct ggggatattc tttggctatg
     9421 cggcttacta tttggttcgt aagaactttg cgcttgctat gccttatctg gtcgagcagg
     9481 gattctctcg cggtgattta ggtttcgcgc tttcggggat ctcgatcgct tatggatttt
     9541 cgaaattcat aatgggttcg gtatcagatc gctcgaatcc gcgcgttttc ctccccgcag
     9601 gtttgattct ggcggcggca gtgatgttgt ttatgggctt tgtgccatgg gcgacgtcga
     9661 gcattgcggt gatgtttgta cttttgttcc tctgcggttg gttccagggg atggggtggc
     9721 cgccgtgtgg tcgtactatg gtgcactggt ggtcgcagaa agaacgtggc ggcattgtgt
     9781 cagtgtggaa ctgtgcgcac aacgttggtg gtggtattcc gccgctgctg ttcctgctgg
     9841 ggatggcctg gttcaatgac tggcacgcgg cgctctatat gcccgctttc tgcgccattc
     9901 tggtggcatt attcgccttt gcgatgatgc gcgatacccc gcaatcctgt ggcttgccgc
     9961 cgatcgaaga gtacaaaaat gattatccgg acgactataa cgaaaaagcg gaacaggagc
    10021 tgacggcgaa gcaaatcttc atgcagtacg tactgccgaa caaactgctg tggtatatcg
    10081 ccatcgccaa cgtgttcgtt tatctgctgc gttacggcat cctcgactgg tcaccgactt
    10141 atctgaaaga ggttaagcat ttcgcgctgg ataaatcctc ctgggcctac ttcctttatg
    10201 aatatgcagg tattccgggc actctgctgt gcggctggat gtcggataaa gtcttccgtg
    10261 gcaaccgtgg ggcaaccggc gttttcttta tgacactggt gaccatcgcg actatcgttt
    10321 actggatgaa cccggcaggt aacccaaccg tcgatatgat ttgtatgatt gttatcggct
    10381 tcctgatcta cggtcctgtg atgctgatcg gtctgcatgc gctggaactg gcaccgaaaa
    10441 aagcggcagg tacggcagcg ggctttaccg ggctgtttgg ttacctgggc ggttcggtgg
    10501 cggcgagcgc gattgttggc tacaccgtgg acttcttcgg ctgggatggc ggctttatgg
    10561 taatgattgg cggcagcatt ctggcggtta tcttgttgat tgttgtgatg attggcgaaa
    10621 aacgtcgcca tgaacaatta ctgcaaaaac gcaacggagg ctaatggcat gaaattgacg
    10681 ctgaaaaacc ttagcatggc gatcatgatg agcactatag tcatgggaag cagtgcaatg
    10741 gcggcggaca gcaacgaaaa aatagtcatc gcccatcgcg gtgccagtgg atatttgccg
    10801 gagcatacgc tgccagcaaa agcgatggcg tatgcgcagg gagcggatta tctggaacag
    10861 gatttggtga tgaccaaaga cgaccatctg gttgttctgc atgaccatta tctcgatcgt
    10921 gttactgatg ttgccgatcg tttcccggat cgggcgcgca aagacggtcg ttactacgcg
    10981 atagatttca cgctggatga aattaagtcg ctgaaattta ccgaaggttt cgatattgaa
    11041 aacggtaaaa aagtgcagac ttatccgggg cgtttcccaa tgggtaagtc cgacttccgg
    11101 gtgcacacct ttgaagaaga gattgaattt gttcaggggt taaatcactc taccgggaaa
    11161 aatatcggta tttatccaga aatcaaagcg ccgtggttcc atcatcagga agggaaggat
    11221 attgcggcaa aaacgctgga agtgctgaag aaatatggtt acaccggtaa agacgataaa
    11281 gtttatttgc aatgttttga tgctgatgag ctgaagcgta ttaagaatga gctggaaccc
    11341 aaaatgggca tggagctcaa tctggtacag ctgattgcct ataccgactg gaatgaaacg
    11401 cagcagaaac agccggatgg aagctgggtt aattacaact acgactggat gtttaagccg
    11461 ggtgccatga aacaggtggc ggaatatgca gatggtattg gtccggatta ccatatgttg
    11521 attgaggaaa catcgcagcc gggtaatatc aaactcactg gcatggtgca agatgctcag
    11581 cagaataaac tggtagtgca tccttatacc gtgcggtcgg ataaactgcc tgaatacact
    11641 actgatgtga atcagttata tgatgctctg tataacaaag cgggtgtaaa tgggctgttt
    11701 actgatttcc ctgataaggc agtaaaattc cttaataaag agtaatgcta ttgcaggatg
    11761 gcgttcatgc gccatcctct gctaaagcga aataatgcta ctaccgttgt tctattcatt
    11821 aaccggtgca tcctgttaaa aaaatgccaa catatttgcg taaaactcat tcgttaggga
    11881 agctaacact gggtattgtt attctttaaa cagtatgaat t
//
LOCUS       DBANUU010000131        11854 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000131 DBANUU010000000
VERSION     DBANUU010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.132"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            158..1378
                     /gene="ybdN"
                     /locus_tag="ACIE68_003718"
     CDS             158..1378
                     /gene="ybdN"
                     /locus_tag="ACIE68_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFX5909971.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1351..1980
                     /gene="ybdM"
                     /locus_tag="ACIE68_003719"
     CDS             1351..1980
                     /gene="ybdM"
                     /locus_tag="ACIE68_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFX5909972.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1981..3141)
                     /gene="ybdL"
                     /locus_tag="ACIE68_003720"
     CDS             complement(1981..3141)
                     /gene="ybdL"
                     /locus_tag="ACIE68_003720"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFX5909973.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3250..4338
                     /gene="hcxA"
                     /locus_tag="ACIE68_003721"
     CDS             3250..4338
                     /gene="hcxA"
                     /locus_tag="ACIE68_003721"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFX5909974.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4348..4545)
                     /gene="ybdD"
                     /locus_tag="ACIE68_003722"
     CDS             complement(4348..4545)
                     /gene="ybdD"
                     /locus_tag="ACIE68_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFX5909975.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4727..6832)
                     /gene="cstA"
                     /locus_tag="ACIE68_003723"
     CDS             complement(4727..6832)
                     /gene="cstA"
                     /locus_tag="ACIE68_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFX5909976.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7013..7426)
                     /gene="entH"
                     /locus_tag="ACIE68_003724"
     CDS             complement(7013..7426)
                     /gene="entH"
                     /locus_tag="ACIE68_003724"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFX5909977.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7429..8175)
                     /gene="entA"
                     /locus_tag="ACIE68_003725"
     CDS             complement(7429..8175)
                     /gene="entA"
                     /locus_tag="ACIE68_003725"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFX5909978.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8175..9032)
                     /gene="entB"
                     /locus_tag="ACIE68_003726"
     CDS             complement(8175..9032)
                     /gene="entB"
                     /locus_tag="ACIE68_003726"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFX5909979.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9046..10656)
                     /gene="entE"
                     /locus_tag="ACIE68_003727"
     CDS             complement(9046..10656)
                     /gene="entE"
                     /locus_tag="ACIE68_003727"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFX5909980.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10666..11841)
                     /gene="entC"
                     /locus_tag="ACIE68_003728"
     CDS             complement(10666..11841)
                     /gene="entC"
                     /locus_tag="ACIE68_003728"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFX5909981.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
BASE COUNT         2843 a         3397 c         3013 g         2601 t
ORIGIN      
        1 agagtttttg atatataact acagacagct actataactt tcatctattt attcacagcg
       61 cgaatgaaaa ataaaacact ctgctttaca gtgtttactt tataattaac agtcttatct
      121 cactgttcac cctgtaacgt tcagagagcg tcttcagatg tctatttata aaattcctct
      181 tccgctcaat attctggaag ctgcacgaga gcgtattacc tggacactca acaccctacc
      241 ccgcgtttgt gtctcatttt ctggcggcaa agactctggt ttaatgctgc atctgacagc
      301 cgaacttgcc cgacaaatgg gcaaaaaaat ctgcgttttg tttatcgact gggaggcgca
      361 attctcttgc accattaact atgttcagtc cctgcgcgag ttgtacaccg atgtcatcga
      421 agagttttac tgggttgcgc tcccgcttac gacgcaaaat tccctttcac aataccaacc
      481 cgaatggcag tgctgggaac ctgatgtcga atgggtgcgt cagcccccgc aagatgcgat
      541 aaccgatcct gactttttct gcttttacca gccaggcatg accttcgaac aatttgtacg
      601 tgagtttgcc gaatggtttt cacaaaaacg tccggcggcg atgatgatcg gcatccgtgc
      661 ggatgagtcc tacaaccgtt ttgtcgccat cgccagttta aataaacaac gttttgccga
      721 cgataaaccc tggactacgg ccgcaccagg cggtcatagc tggtacattt accccattta
      781 cgactggaaa gtggctgata tctggacctg gtatgcaaat catcaaagtc tctgcaaccc
      841 actgtataac ctaatgtatc aggcaggcgt tcctctgcgt catatgcgaa tttgcgaacc
      901 ttttggcccg gagcaacgac aaggattatg gctctatcac gttatcgaac cggatcgctg
      961 ggctgctatg tgcgcacgag tcagcggggt aaaaagtggc ggcatttacg ccggacatga
     1021 caatcatttc tatgggcatc ggaaaatcct caagccagaa catttagact ggcaagaata
     1081 tgcattattg ctgctcaata gcatgccgga aaaaacagct gagcattacc gcaataaaat
     1141 cgccatttat ttgcactggt atcagaaaaa aggcatcgag gttccacaaa cccagcaagg
     1201 ggacattggc gcgaaagata tcccctcctg gcggcggata tgcaaagttt tactcaataa
     1261 cgattactgg tgtcgggcat tatcatttag tcctacgaaa gcgaagaact atcagcgtta
     1321 taacgaacgg ataaaaggaa aacgtcagga atgggggata ctatgcaaca acgattaacg
     1381 caggatttaa cgcaatttct cgccagcttg ccggaagatg atcgcatcaa cgcgattaat
     1441 gaaatccgca tggcgatcca tcaggttagc ccctttcgtg aagagccagt ggattgcgtt
     1501 ctgtgggtta aaaacagcca acttatgcct aatgattaca acccaaacaa cgtggcacca
     1561 cccgaaaaaa aactgctgaa aaaatccatt gaaatagatg gttttaccca acccattgtg
     1621 gtcacgcata cggataaaaa tgccctggaa atcgtggatg gttttcaccg ccatgaaatt
     1681 ggtaaaggct catcatcatt aaaattacgc ttaaaaggtt atttgccggt gacctgtctg
     1741 gaaggtacgc gtaatcaacg catcgccgcg actattcgtc ataatcgcgc tcgtggacgc
     1801 catcaaatca ccgccatgtc ggaaatcgtc cgtgaactta gccagttggg atgggacgat
     1861 aataaaattg gcaaagagct gggaatggac agcgacgaag tgctgcgcct gaagcaaatt
     1921 aacggtctgc aagagctgtt tgccgaccgt caatattccc gcgcctggac tgtcaaataa
     1981 ctacagctgg cgcagtcgtt cagctgctgc cagcaacgtc gattccttct tggcaaaaca
     2041 gagacgaatc agtttatgag ggaagggatc ggcgcaaaac accgatagcg gaatcgccgc
     2101 tacgccgtgc tcctgcgtca gccactggca aaactcaaca tcatccaggg tagatacggc
     2161 gctgtaatcc accagcaaaa agtatgtacc ttcacacggt aaaatctcca gccggctttc
     2221 atttaaagca ttcaccagaa tatcgcgctt ctggcgataa aagtccggta acgcaagata
     2281 atgctcaggt tctgcacgta gcatatcagc aagcgccagc tgtgccgggg tattcaccga
     2341 aaaggtcaga tactgatgta ccttgcgaat ttcggcgctg atgggcgctg gcgcaacaca
     2401 ataacccact ttccagccgg tcatatgata ggtcttgcca aatgaagaaa ccgccactgc
     2461 ccgctcacgc agctgcggat gcgccagcac actggcatgg ccctgttgtg aaaagttgat
     2521 gtgctcgtag acttcatcgc taatgacaaa aatctcgtgc ccggcgatcg cctgccacaa
     2581 agcggcgaaa tcagcctgct gccagacagt tgcactgggg ttatgcggag tgttgaggat
     2641 caccagtctg gtgcgctcgc ttaacaatgc ggcaaattcc tgccagtcaa cgcgaaaatg
     2701 cggtggttgc agtgccatac gcttcactat tcccccagaa agcgcgatgg cgggggagta
     2761 actgtcatag ctgggatcaa aacaaatcac ttcatcgcca ttgcgcacca gtgcggtaat
     2821 cgccgcgtat aacgcttccg tcgcccctgc cgttacggtg atatcgctat cggcatctgg
     2881 ttgatagcca tacaaacgtt ccgttttctg agcaatcgcc tcgcgcaagg cctgcacgcc
     2941 ggtcatgggc gcgtattggt ttgccccctg ggcaacgtgg tacgccagcc gctcctgtaa
     3001 atagcgcgga ccatcaaaat caggaaagcc ttgcgacagg ttaatcgcct ggtgttgctg
     3061 cgccagcgcg ctcatctggg tgaaaatagt ggtgccaagt tgtggaagtt tgctttgtgg
     3121 aatcagaggg ttatttgtca tttttattgt acctgacgaa gttgcgtgtt gaaggcacta
     3181 taacacgatg ctagtatttg gcaatcaaga cgtttagatg tctaaataaa acaataagga
     3241 caacacaaca tgcctcacaa tcctatccgc gtggtcgtcg gcccggctaa ctacttttca
     3301 catccaggaa gtttcaatca cctgcacgat tttttcactg atgaacaact ttctcgcgcg
     3361 gtgtggatct acggcaaacg cgccattgct gcggcgcaaa ccaaacttcc gccagcgttt
     3421 ggacttccag gggcaaagca tattttgttt cgtggtcatt gcagcgaaag cgatgtacag
     3481 caactggcgg ctgagtccgg tgacgatcgc agcgtggtga ttggcgtcgg cggcggcgca
     3541 ctgctcgaca ccgcgaaagc cctcgcccgc cgtctcggtc tgccatttgt tgccgctccg
     3601 acgatcgccg ccacctgcgc agcctggaca ccgctctccg tctggtataa cgatgccgga
     3661 caggcgctgc attatgagat tttcgacgac gccaatttta tggtgctggt ggaaccggag
     3721 attatcctca atgcaccgca acaatatctg ctggcgggga tcggtgacac gctggcgaaa
     3781 tggtatgaag cggtggtgct ggctccgcaa ccagaaacgt tgccgctaac cgtgcggctg
     3841 gggatcaata atgcgcaggc cattcgcgac gtcttgttaa acagtagcga acaggcactt
     3901 gccgatcagc aaaatcagca gttaacgcaa tcattttgcg atgtggtgga tgctattatt
     3961 gctggtggtg ggatggtcgg tggtctgggc gatcgtttta cgcgtgtggc ggcggctcat
     4021 gccgtgcata acggtctgac tgtcctgccg caaaccgaga agtttcttca cggcaccaaa
     4081 gtcgcttacg gaattctggt gcaaagcgcc ttgctgggtc aggatgatgt gctggcgcaa
     4141 ttaaccgggg cgtatcagcg ttttcatctg ccgactacgc tggcggagct ggaagtggat
     4201 atcaataatc aggcggagat cgacaaagtg attgcccaca ccttgcgccc ggtggagtcc
     4261 attcattacc tgccagtcac gctgacacca gatacgttgc gtgcagcgtt cgaaaaagtg
     4321 gaatcgttta aagcctgacg tgcaaattta gcagcagcgc gcgccgcctt ttccaccgta
     4381 gcgcgcgtcc tgccgctcac ggaaaaactc ctcgtaggtc atcggcgttt gatcgggatg
     4441 gttaacccgc atatgttcga catagttgtc gtaatcaggc ataccaatca tcagcttcgc
     4501 cgcctgacct aaatattttc cggcttttgc cagtgaatca aacatcttgg cctccgctaa
     4561 ccatgttgct gaaagagtgc cggatgcggc gtgaacgcct tatccggcct acaaatgcta
     4621 tcaacgcgct cggtcagtcc cctcgcccca tcggggagag ggttagggtg aggaggaacc
     4681 ctcggcacta tgccaacatt cgccaacatc ccccctcact ctgactttag tgtgcgcctt
     4741 ttgcctgcgc cacgatctcc tcgacatttt ccggcattgg ctcatacggc gtttctttcg
     4801 ccgttggctt cggatctttc aatgccgcca gtgccgtctt aatcgagaac agcgccagaa
     4861 ccaccacgac caccataaag aagatggtta acccggcatc cagacggttg ttgaacacca
     4921 gttgcgccag ctgtgactca gtatactgcg acggaatatt gccgctgtcg atcattgcct
     4981 ggaacttatt agcaatggcc aggaagccga ctttcgcatc cgggctaaac gctttctgcc
     5041 agcctgcggt cagggtacaa atcagcagcc aggccgttgg taccagcgcc acccaggcgt
     5101 aacgttgacg tttcatcttg aacaacacca cggcacagag catcagcgcc atccctgcca
     5161 gcatctggtt ggcaatacca aacagcggcc acagagtgtt aatgccgccc agcggatcga
     5221 ccacgccctg atggaggaag tagccccacg ccagtacgca cagcgctgtt gccagcaggt
     5281 tagcaggcag tgaatcggtc cgcttcaggc ccggagaaac cacgcccagc agatcctgca
     5341 acataaagcg cgcagcacgc gtacccgcat ctaccgccgt cagaataaac agagcttcaa
     5401 acagaatggc gaagtgatac cagaacgcca catccatcat accacccagc gcaccgtgca
     5461 ggatgtaagc catccccacc gccagcgtcg gcgcaccgcc cgcacgggaa atgatcgact
     5521 gttcacccac ttcgctggca atctggttga gcgtatctgg tgtaatgcta aagccccagc
     5581 tactcaccac ctgcgcggca gaagcgacta catccgccgt ccctgccgga gccagcaccg
     5641 ccattgggct gttcatggca aaatacacgc ccggatcgat gatacaggca gaaaccagcg
     5701 ccataatcgc cacgaaggat tccatcaaca tcccaccgta gccgataaag cacgcctgcc
     5761 cttcgttcgc cagcatcttc ggcgtggtgc cggaagagat cagcgcatgg aagccagaca
     5821 ccgcaccaca ggcgatggtg ataaacagga acgggaacag gttaccggtc cataccgggc
     5881 cagtgccatc gacaaatttg gtcagcgcag gcatggtcag cgtcgggcgc ataatcaaaa
     5941 tgcctaccgc cagaccgacg atagtcccga ttttcaggaa ggtcgagaga tagtcacgcg
     6001 gggccagcag caaccacacc ggcagcaccg ccgccacaaa accgtagccc accagcatcc
     6061 aggtcagctg cacgccggta aagtcaaagt acggtgccca ggtcggactt tctgccaccc
     6121 agccgccaga gataatggcg aaaatcagga acaccaggcc aatcactgac acttcgccaa
     6181 tgcggcccgg acgcagatag cgcaggtaga ttcccataaa aatcgccagc ggaatggtga
     6241 acgcaacggt atacgttccc cacgggctat gagtcagggc tttcaccacg atcatcgcca
     6301 gtactgcaag gataatgacc atgatcataa agcaggctac cagcgcaatc accccggcag
     6361 ttggccccat ctcttctttg accagctcac ccagcgatcg accgtcacgg cgcgtagaaa
     6421 caaacagcac catgaaatcc tgcaccgcac cagcgagaac caccccggcg agcagccaga
     6481 tcatccctgg caggtaaccc atttgcgccg ccagtaccgg cccaaccaac ggacctgctc
     6541 cggcaatggc cgcaaaatgg tgaccgaaca gcactttctt gtccgtcggc acatagtcca
     6601 gcccgtcgtt atggcgcacc gctggcgtca tacgcgtcgg gtcaaccgcc agcacatttt
     6661 tggcgatata cagcccataa aaacggtaag cgatcagata gatacagacc gacgccacca
     6721 caatccacag cgcgttgatc tgttccccac gatttaaagc aatgtatccc agagcaaatg
     6781 ctcccattac agagagcact gtccagacga ggtatttccc tgatttgttc atagttgtta
     6841 tccgtgtgcg tgtccataga gatgttacat tttgtttcta taacatcttt gtacgattta
     6901 acaacacggt aaccatgtaa atcggagttg aaaccaatat ttaacttaac aatgttaact
     6961 cgatcactcc gttaaccgag ttttcctgca atctcattta attctgtcgg gctcatccca
     7021 aaactgccgt acccagccga caagtgcagc aacgccgccc ctgttcatcg aaaacgacga
     7081 tttcccagct ctgattttgc cgcccaagat gcagcggctg gcagacgccg cgtaccttgc
     7141 cttcagacac cgggcgatga tgggtggcgt taagttccgt acccaccaca cactgcccgt
     7201 cacgggtcat cataaatccg gccatcgatc ccagcgtttc agccagcgcc gccgacgcgc
     7261 cgccatgcag caggccaaac ggctgatgag tacgggtatc aaccggcatt tcggcttcca
     7321 gcacatcatc tcccagacgg gtatacacaa ttcccagatg cgccaccatt gtgttatcgc
     7381 tggtggcgtt cagttcgtcg agcgttaaat ggcgtttcca gatcatgctt atgcccccag
     7441 cgttgagccg ccatcgacca caatatcctg tagggtgata tggctggcga ggtcagaggc
     7501 gaggaacaaa atcgtgttgg cgatctcttg tggacgggcg attttcccca gcggaatgcc
     7561 gagtttaaac tgctcgccaa agccgcgaat acgctgttct tcggcgtcat cgctcaccca
     7621 cagcgtgcgt tgcatatcgg tgtcggtgga gccaggcgaa accacattac agcgcacgcc
     7681 gctacccgcc agttccagcc cgacgctcaa cgccaggctt ttcagcgccg ctttcgatgc
     7741 gccataagca ctcataccaa tacgcggcgt gtgtgcggcg tcggacgcca cagtgacaat
     7801 cgccccgccc cgctgacggc gaaactggtt catggtttgc tggaacaggt taaacgcacc
     7861 gccgacgtta accgcaaaag tctgctgcca gtcctcttta ctgagctgat cggtcgcgcc
     7921 catgcgcaaa attcccgccg cattgatcag cacgtccagt cgctccgttt cagctaacag
     7981 tcgctgacat acctgcgcca cctgcccggc gtcggcaaca tccattactt cggtcgcaaa
     8041 gggatattgc tcctgagtga acgcttgatc aaaacctgta actttcgctc ccgcctcaac
     8101 aaacgccagc gccgtggcgt agccgatacc tttacctgcg ccggttaccc agacattttt
     8161 accgctgaaa tccattattt cacctcgcgg gagagtagct tccaccaggc gtcgatggtc
     8221 gggtttttcg ccagcatgac aaagtcgata tcaccatgca ctttgcgcca gcgcgccgcc
     8281 agcgccatca tgcgcaccga atccagaccg tagtcgatca ggttgtcgtc atcgaacggt
     8341 tcatcggact cgtccagcaa cggcaggata acctcacgca gcgccgcttt gctggcgggg
     8401 acaggtgctg gcagtaattc ttcagtcatc accacccggc cagaccgccc ggccacatat
     8461 ttcagcgaca tcaaatgctc gtcacggctg aaatcggcca gcgcatccgc caccataaac
     8521 ggtttaatat cgcgcataaa tgcgtcggtt gcggtggtca tacagccaat gtgggcatat
     8581 accccggtaa taatcagctg gttacgtcca ctctctttga gcatttgctc cagcggagaa
     8641 cgatgaaacg cgctgtagcg ccacttcacc agcaccgtgt cgtcggcatc tggcgtcagg
     8701 cgatccacca ccttttgctg ttccggcgaa cgggtcaggc ccggccccca catatcattc
     8761 aacagcgccc gatcttcatc gctctgctct ttcggctggg cggtgtaata aaccgggata
     8821 ttgtgctgtt tgcagtagtc gcgcagcgca gcaatattcg cgatcacctg ctccatcatc
     8881 gggcagttct cgccccagaa gctgacaaaa tagtcctgca tatcatggat taacaacgcg
     8941 gcacgttgcg gttcaaaggc ccagtcaact ttattctgcg gaatatcgtg agactccggc
     9001 agtgcgtaag cctgtaattt tggaatagcc atagtgttct ctccttcagg ctgatgcgcg
     9061 tgacgccagc cactgacgta attgtttttt atcgactttc ccgaccgccg tcagcggaag
     9121 tgaatccaca cactccacgc gatccggtaa tttaaattcg gcaataccct gttcacgcag
     9181 gaaacgacgc acctgcaccg cgcgcagcgg ctcttttacc accagataag cgcagctttt
     9241 ttcgcccatc agctcatctt ccatgctcac cagtgcggca tagatcactg ccggatggcg
     9301 cagcagcagg ttttcgatct cttcggcagc gatcttctcg ccgccacggt tgatctgatc
     9361 tttctcgcgc ccctgcacgg tgatgtaacc ctctggatca atagagatca gatcgccgga
     9421 acagtaaaaa ccgttggcat caaaggcgct ggcattgtgc tgcggacttt tgtaataacc
     9481 gcggaaggtg tacggcccgc gcgtcatcag gcgtccgact tccccttgcg gcagtggatt
     9541 tccttcggca tcggcaaccc acacttcatc gtccggacac attgggtaac cctgggtatg
     9601 gataattttc tccgcgctat catcaagacg ggtgtagttc accagccctt ccgccatgcc
     9661 aaacacctgc tgcaactgac agccaatctc agcgggaata cgcgccgcaa gggtggcaga
     9721 aagacgtgcg ccgccgacct gtaacagttt cagcgaggca agctgcgccc ggctttcgcc
     9781 ttcggtcagc gcctgcaacc acaggctgac tgcgggtggc accagcgcgg taacgttaac
     9841 ctgatgtttt tcaatcaatg ggaaacagag cgtggcgctg ggatcggccg ccagaacaac
     9901 cgttcctccg gcaagaaaga cgcccagcga tcccggcgaa ctcatggcgt agttatgagc
     9961 cgccgggatc gcgcacaggt agcgtgtctg ttgtgtgaac tgacaaatct cgacgctacg
    10021 acgcacgctg tagtagtagt cgttatgagt gcgcgggatc agtttcggtg tgccggtggt
    10081 gccgccggaa agctggaaat aggccacttc atcagcaggt gatggcgtgg cagtaaaatc
    10141 ctcagccgga tggttaatcg catcctgcaa gttatgctca ccgctgtcgt tgtgcagttg
    10201 caccacgcga atggaggaat gttctgtgac gaaagtattg aggaaatcat ccccgctaaa
    10261 cagcgcatgt tggcgatcgg caatcagcaa tgcgggttca atctggctgg cataggcgtt
    10321 cagttcacta cgctgatggc tgaacagcgc cagcaccggc gcaacgccca gtttcagcag
    10381 cgcgaaaaag gtaatataca attcagcgac gttacccagt tgtaccagcg cggtttcacc
    10441 aggtttaatg ccctgacggc gtaaactaca cgcgaggtta tccgccgcct gattcagctc
    10501 ccggtaactc aactgtcgct cgccgtcgat aaccgcgatg ctgtcactcg cagcgtggcg
    10561 agtcagaatg tcggtcagcg gcaaatcctg ccagtagcct ttttcccgat agcgacgggc
    10621 aaactcttcc ggccagcggg tgaatggaat gctcatcctc gctccttaat gcaatccaaa
    10681 aacgttcaac atggtagaaa gtttgacgcc tgtttcgcgc cactcaccca acggtgacga
    10741 cgcaggcaca atccccgctc cggcaaacag acgcacctga ttttcccgca gcttcgcgca
    10801 gcggatggtc accacccatt cgccgttacc ttcgctgtca caccaaccca caatgccgcc
    10861 aaacagttcg cggtcgaatg gctccagttc agcaataacc tgggtcgcgg cctgatgcgg
    10921 gaaaccgctc agcgcggggg ttggatgcag cagacaggcc agagtcagtg cgttttcttg
    10981 cgaattcgct ttaccttcaa agggagttgc gagatgccac agcgtcggcg tggtaatcaa
    11041 ttgtggagag gagggaacgt gtaactcact actgcgttcg cgcagtacct ctttcatcgc
    11101 ctgagtcacc agttcatgtt catggcgatc tttttctgac gccagcagac gattacccgc
    11161 ttcgcgatcg agcacttcat ccggctgacg acgcgcggaa ccggctaacg gaatggagct
    11221 aaaacgctcg ccgtctttac gtagcagcag ttccgggctg gcccccagca ggacgccacc
    11281 atcagccagc gggacatgga agttgtaact aaccgggttt tgcgcaatca accgttccag
    11341 caatacgcca ctatcaatgg cggcgtcagt ggtgatatca atcaatcgtg acaacaccac
    11401 tttgtcgacc tgcggcgtgg cggtaagtgc ggcggcgcga gcaaccatct gttcaaacgt
    11461 ggtttgttca ggaattgcct ggcgttccac cacgttcagc gactggctgc gggtaaaacg
    11521 gcgggctgag gtctgttttt cctggcggga gaacgactgc caggattcgg gaatatacag
    11581 cgacgaaggc tgacgtggat cgaagggaat cgccccgacc atcaccggat ttttgatgcc
    11641 ctgcgctttg gcatcggcaa acagcgcggc gagtttttgc tggaagggac tgtcgggcga
    11701 atccccgttc acagccggtt catcgaagcg ggcgaaacat cctgacgtcg taaaactgcg
    11761 gtacggcgac ataaagaaaa agcgattggg cgcaagtgtt gccatggtct gctgtacttc
    11821 ctcagccagt gacgtatcca tatcatcctc caca
//
LOCUS       DBANUU010000132        11824 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000132 DBANUU010000000
VERSION     DBANUU010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.133"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..98
                     /locus_tag="ACIE68_003729"
     CDS             <1..98
                     /locus_tag="ACIE68_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5909982.1"
                     /translation="TYCNNDYYNQAIREEPIDLLDRSFSSSSWPF"
     gene            422..1810
                     /gene="narU"
                     /locus_tag="ACIE68_003730"
     CDS             422..1810
                     /gene="narU"
                     /locus_tag="ACIE68_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFX5909983.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1892..5632
                     /gene="narZ"
                     /locus_tag="ACIE68_003731"
     CDS             1892..5632
                     /gene="narZ"
                     /locus_tag="ACIE68_003731"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFX5909984.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSSSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5629..7173
                     /gene="narH"
                     /locus_tag="ACIE68_003732"
     CDS             5629..7173
                     /gene="narH"
                     /locus_tag="ACIE68_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFX5909985.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7173..7868
                     /gene="narW"
                     /locus_tag="ACIE68_003733"
     CDS             7173..7868
                     /gene="narW"
                     /locus_tag="ACIE68_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFX5909986.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7865..8545
                     /gene="narI"
                     /locus_tag="ACIE68_003734"
     CDS             7865..8545
                     /gene="narI"
                     /locus_tag="ACIE68_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFX5909987.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8624..9517
                     /gene="yddE"
                     /locus_tag="ACIE68_003735"
     CDS             8624..9517
                     /gene="yddE"
                     /locus_tag="ACIE68_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFX5909988.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9613..10458)
                     /gene="nhoA"
                     /locus_tag="ACIE68_003736"
     CDS             complement(9613..10458)
                     /gene="nhoA"
                     /locus_tag="ACIE68_003736"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFX5909989.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIAHRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10631..11200
                     /gene="yddH"
                     /locus_tag="ACIE68_003737"
     CDS             10631..11200
                     /gene="yddH"
                     /locus_tag="ACIE68_003737"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFX5909990.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11204..11431)
                     /gene="pptA"
                     /locus_tag="ACIE68_003738"
     CDS             complement(11204..11431)
                     /gene="pptA"
                     /locus_tag="ACIE68_003738"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFX5909991.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11482..11824)
                     /locus_tag="ACIE68_003739"
                     /pseudo
     CDS             complement(11482..11824)
                     /locus_tag="ACIE68_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         2788 a         3063 c         3079 g         2894 t
ORIGIN      
        1 atacttactg taataacgat tattacaatc aggcaataag agaggaaccg atagaccttc
       61 tggacagatc gttttcctcc agttcatggc ctttttagtg aataaatcat cactaattct
      121 ataagtgtct ggtcttgttg ataatatagt taatatgtaa ccctttagat tcacgatctg
      181 aagggttgaa acatgacata cgttcaaaga ttaacccccg tttgaaggtt agcttgttag
      241 ttacatttag taacacatat ctggctactt aatccccatt ctgcccccgc ggttacgctt
      301 tcttatctat tctttcaatc agcacttcac ccaattcatc cttaattcta ataactggct
      361 attgcgcctt acctcctgtt ttgtcttttt tcattaccaa tgtgtgcatg tgaggaacaa
      421 tatggcactg caaaatgaga aaaatagtcg ttatctttta cgcgactgga aaccagaaaa
      481 tcctgccttc tgggaaaata aaggaaaaca tattgctcga agaaacctct ggatatcagt
      541 cagttgtcta cttcttgcct tctgtgtctg gatgctattt agcgcagtta ccgttaatct
      601 caataaaatc ggttttaatt ttactaccga tcaactcttt ttattaaccg cattaccctc
      661 cgtttctggc gcattattgc gtgttcccta ctcctttatg gtgcctatat tcggtggacg
      721 ccgatggacg gtttttagta ctgcaatcct gattattcct tgcgtctggc tcggaattgc
      781 cgtgcaaaat ccgaatactc cttttgggat atttatcgtt atcgctttgc tatgcggttt
      841 tgcaggtgca aactttgctt cgagcatggg caatatcagt ttcttctttc caaaagccaa
      901 acaagggagc gctcttggga ttaatggcgg attaggaaac ttaggtgtaa gtgtaatgca
      961 gctggttgca ccgctggtca tttttgtacc tgtatttgcc tttctcggcg tcaatggcgt
     1021 accgcaggcc gacggttcgg tgatgtcgct ggcgaatgcc gcatggattt gggtaccgct
     1081 actggcgatt gccacgatcg ctgcctggtc agggatgaat gatatcgcca gttcacgcgc
     1141 ctcaattgcc gaccagctcc ctgtcttaca acgcctgcat ctctggctgc tgagcctgct
     1201 ttaccttgcc accttcggtt cgtttatcgg tttttctgcg ggttttgcca tgctggcaaa
     1261 aacccagttc ccggatgtga atattctgcg cctggcgttc tttggcccat ttatcggtgc
     1321 catcgcgcgg tcggttggtg gtgctatttc cgataagttc ggcggcgtgc gggtgacgtt
     1381 gatcaacttt atttttatgg cgattttcag tgccctgctg ttccttacct taccgggcac
     1441 aggctccggt aatttcatcg ccttttacgc cgtatttatg gggctgtttc tgactgcggg
     1501 tctgggaagt ggttctactt tccagatgat cgccgtcatc tttcgccaga taaccattta
     1561 tcgggtaaag atgaaaggcg gtagtgatga gcaagctcag aaagaagccg tcaccgaaac
     1621 ggcggcggct ctgggcttta tctcagccat tggcgcagtg ggcggctttt ttattccgca
     1681 ggcgtttggc atgtcgctca atatgaccgg ctctccggtc ggcgcgatga aagtgttttt
     1741 aatcttctac atcgtttgtg tgctgctgac ctggctggtt tatggtcggc ggaagttcag
     1801 ccaaaaataa gagatcgatg cctgatgtac aaccgtgtcg catccgacaa ggcatcagat
     1861 cccaattcat tcctggagca ggagtcatgt catgagtaaa cttttggatc gctttcgcta
     1921 cttcaaacaa aagggcgaaa cctttgccga tggtcacgga caggtgatgc atagcaaccg
     1981 cgactgggag gacagctatc gccagcgttg gcagttcgac aaaatcgtgc gttccaccca
     2041 cggtgttaac tgtacaggct cctgtagctg gaaaatctac gttaaaaatg gtctggtgac
     2101 ctgggaaatc caacagaccg actacccgcg cactcgccct gacctgccca atcatgaacc
     2161 tcgcggctgc ccgcgtggcg caagttactc ctggtatctt tacagcgcta accgcctgaa
     2221 atacccgctc attcgtaaac gactgattga actgtggcgc gaagccctca agcaacacag
     2281 cgatccggta ctggcgtggg catcgattat gaacgatccg caaaagtgcc tgagctacaa
     2341 acaagtgcgt gggcgcggcg ggtttatccg ctccaactgg caggaactaa accagctgat
     2401 tgccgccgct aacgtctgga ccatcaaaac ctacggcccg gatcgcgttg ccggtttctc
     2461 gccgatcccg gcgatgtcga tggtttctta cgccgccgga acgcgttatc tgtcgctgct
     2521 tggcggcacc tgtttaagtt tctacgactg gtattgcgac ctgccgcccg cctcgccgat
     2581 gacctggggc gagcaaaccg acgtaccgga atctgccgac tggtataact ccagctacat
     2641 catcgcctgg gggtctaacg taccgcagac acgtacgccg gacgcccact tctttaccga
     2701 agtacgctac aaaggcacta aaaccatcgc cattacccct gactactctg aagtggccaa
     2761 attgtgcgac cagtggctgg caccgaaaca aggcactgat agcgccctgg cgatggcaat
     2821 gggccatgtg attttaaaag agtttcatct cgataatccc agcgactact ttatcaacta
     2881 ctgccgccgc tacagcgaca tgccgatgct ggtaatgctg gagcctcgcg acgatggtag
     2941 ctacgttccc gggcgcatga tccgcgcatc tgacctggtg gatggactgg gcgaaagcaa
     3001 caatccgcag tggaaaaccg tagcagttaa taccgcaggt gaattggtag tgccgaacgg
     3061 ttcgattggt ttccgctggg gagaaaaagg caaatggaat ctggaatcca ttgccgccgg
     3121 tacggaaacc gaattgtcgt taaccctgct cggtcaacat gacgctgttg caggcgtggc
     3181 cttcccctac tttggcggca ttgaaaatcc gcattttcgc agcgtaaaac acaatccggt
     3241 gctggtgcgc caattgcccg ttaaaaacct gacgttagtc gatggcaaca cctgtccggt
     3301 ggtcagcgtt tatgatttgg tactggcgaa ttacggcctc gatcgcgggc tggaagatga
     3361 aaacagtgcg aaagattacg ctgaaatcaa accgtacacc ccagcctggg gtgagcaaat
     3421 taccggcgtg ccgcgccagt atattgaaac catcgcccgt gaatttgccg ataccgccca
     3481 taaaacgcat gggcgctcga tgattatcct cggcgcaggt gttaaccact ggtatcacat
     3541 ggacatgaac taccgcggga tgatcaatat gctgatcttc tgcggttgtg tcgggcaaag
     3601 cggtggcggc tgggcacact atgtcggcca ggaaaaactg cgcccacaaa ccggctggtt
     3661 gccactggcc tttgcgctcg actggaaccg accaccgcgc caaatgaaca gcacctcgtt
     3721 tttctacaat cattccagcc aatggcgcta tgaaaaagtc tctgctcagg agttactttc
     3781 accgctcgcc gatgccagta agtacagcgg tcatctgatt gatttcaacg ttcgcgccga
     3841 acgtatgggc tggctacctt ctgcgccgca gctggggcgt aacccgctcg ggattaaagc
     3901 tgaagccgac aaggccggat tatcccccac agaatttacc gcccaggcgc tgaaatcggg
     3961 cgatttacgt atggcctgcg aacaaccaga tagcagcagc aatcatccgc gtaatttgtt
     4021 tgtctggcgc tctaacctgc ttggctcctc cggcaaaggc cacgagtata tgcagaagta
     4081 tctgctgggg accgaaagcg ggattcaggg cgaggaactc ggtgccagcg acgggatcaa
     4141 accggaagaa gtcgagtggc aaactgcagc gattgaaggc aagctcgacc tgctggtgac
     4201 gctcgacttc cgcatgtcca gtacctgcct gttctccgat atcgttctgc ccaccgccac
     4261 ctggtacgaa aaagacgata tgaacacctc ggatatgcat ccgtttattc atccgctttc
     4321 tgcggcggtc gatccggcgt gggaatcacg cagcgactgg gaaatctaca aaggtatcgc
     4381 caaagcattt tcgcaagtgt gcgtgggtca tcttggcaaa gaaaccgacg tggtattaca
     4441 acccctactg catgactctc cggcagagct ctcacagccg tgtgaagtgc tcgactggcg
     4501 caaaggcgaa tgcgatctga ttccaggcaa aaccgcgccg aatattgtgg cggtggagcg
     4561 cgactaccct gctacgtatg aacgctttac ctcgctcggg ccattgatgg acaaacttgg
     4621 caatggcggt aaagggattt cgtggaatac gcaggatgaa atcgatttcc tcggcaaact
     4681 caattacacc aagcgtgatg gcccggcaca ggggcgtccg ctgattgaca ccgccattga
     4741 cgcttcagaa gtgattctgg cactggcacc ggaaaccaac ggtcatgttg cagtcaaagc
     4801 gtggcaggcg ctgggcgaga tcaccgggcg cgaacatacc catctggcgc tgcacaaaga
     4861 ggacgagaag attcgctttc gcgatattca ggcgcagccg cgtaaaatta tctccagccc
     4921 cacctggtcc ggtctggaaa gcgatcacgt atcctataac gcgggataca ccaacgttca
     4981 tgagttaatt ccgtggcgca cgctgtcggg acgccagcag ctctatcagg atcatccgtg
     5041 gatgcgtgct tttggtgaaa gcctggtggc ttatcgcccg cctatcgaca cccgtagcgt
     5101 cagtgagatg cgccagatac cgccaaacgg cttcccggaa aaagcactta acttcctgac
     5161 gccgcaccag aaatggggca ttcactcaac ctacagtgaa aacctgctaa tgctgacgct
     5221 ctctcgcggt ggaccgattg tctggatcag cgaaacagat gcccgagaac tgaccattgt
     5281 cgataacgac tgggtggaag tattcaacgc caacggcgcg ctgactgccc gcgcggtggt
     5341 cagccaacgt gtaccgccgg gcatgaccat gatgtatcac gcccaggaac gcattatgaa
     5401 tattcctggt tcggaagtaa ctggcatgcg cggcggcatt cataactcgg tcacccgcgt
     5461 ttgcccgaaa ccgacgcata tgattggcgg ttacgcgcag ctggcctggg gctttaacta
     5521 ctacggcacc gtcggctcga accgcgacga attcatcatg atccgcaaaa tgaaaaacgt
     5581 taactggctg gatgatgaag gtcgcgatca ggtacaggag gcgaaaaaat gaaaatacgt
     5641 tcgcaagtcg gcatggtgct taacctcgat aaatgtatcg gctgccatac ctgttcggtg
     5701 acctgtaaaa acgtctggac cggacgcgaa ggcatggagt acgcatggtt taacaacgtc
     5761 gaaaccaaac cgggcattgg ttatcctaaa aactgggaag atcaggaaga gtggcaaggc
     5821 ggctgggtcc gcgatgtgaa tggcaagatc cgtccgcgtc tgggcagcaa gatgggcgta
     5881 ataaccaaaa tcttcgccaa cccggtggtg ccgcagattg atgattacta cgaacctttc
     5941 accttcgact acgaacattt gcatagcgca ccggaaggca aacatatccc tactgctcgc
     6001 ccgcgttcac tgattgacgg caaacggatg gacaaagtga tctggggccc aaactgggaa
     6061 gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc gcaacttcga ggccatgcaa
     6121 aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt acctgccgcg cctgtgcgaa
     6181 cactgcctca atcccagttg cgtggcgacc tgcccaagcg gcgctatcta caaacgcgaa
     6241 gaagacggca ttgtgctgat tgatcaggat aaatgccgtg gctggcgttt gtgcattagc
     6301 ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg gcaagtcaga aaaatgtatc
     6361 ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt gctcagaaac ctgcgtgggt
     6421 cgcatccggt atctgggcgt gctgctttac gacgccgacc gcattgagga agcggcgagc
     6481 accgagcgcg aagttgacct ctatgaacgt cagtgcgaag tgttcctcga tccacacgat
     6541 ccctcagtga tcgaggaagc cctgaaacaa ggtattccac aaaacgtgat tgaagctgcc
     6601 cagcgttcgc ctgtctacaa aatggcgatg gactggaaac tggcgctacc gctgcaccct
     6661 gaatatcgca ccctgccaat ggtctggtac gttcctccgc tgtcgccgat tcagtcctac
     6721 gcagatgcgg gcggtttgcc gaaaagcgaa ggcgtgctgc ccgccatcga aagcctgcgt
     6781 attccggtgc aatatctcgc caatatgttg agtgccggcg ataccggtcc ggtactgcgg
     6841 gcgctgaaac ggatgatggc gatgcgccac tatatgcgtt cacaaaccgt ggaaggcgtt
     6901 actgatactc gtgccatcga cgaagtaggc ctgagcgtcg cccaggtcga agaaatgtat
     6961 cgctatctcg ccattgccaa ctatgaagat cgttttgtta tcccgaccag ccatcgggaa
     7021 atggcgggcg atgccttcgc agaacgcaac ggctgcggtt ttacctttgg cgacggttgt
     7081 cacggctcgg acagcaaatt caacctgttc aacagtagcc gtatcgatgc catcaacatc
     7141 accgaagtgc gcgacaaagc ggagggcgaa taatgcagat cctcaaagtg atcggcctgt
     7201 tgatggagta tccggatgag ctgttgtggg aatgcaagga ggacgcgctg gcgttgatcc
     7261 gccgcgacgc gccgatgctt acggatttca cccacaacct gcttaacgcg ccgctgctgg
     7321 ataaacaggc cgaatggtgc gaagtgtttg accgcgggcg caccacgtcg ctgctgctgt
     7381 tcgaacatgt tcatgccgag tcccgcgatc gcggccaggc aatggtggac ctgctggcgg
     7441 agtatgaaaa ggtcggcctg cagctggatt gtcgggaact gcccgattat ctaccgctgt
     7501 atctggagta tttaagcgta ctgcctgacg atcaggcgaa agaaggattg ctcaatgttg
     7561 cgccgatcct cgccctgctt ggcggtcgct taaaacaacg cgaggcaccg tggtacgcgt
     7621 tgtttgatgc tctgctgcaa ctggcgggaa gctccctttc aagtgacagt gtcacaaaac
     7681 aagtaaacag cgaagagcgc gatgacaccc gccaggcgct tgatgcagtg tgggaagagg
     7741 aacaggtgaa gtttattgaa gataacgcca cggcgtgtga cagctcgccg cttaatcaat
     7801 atcagcgacg ctttagccag gatgtcgcgc cgcaatatgt cgacatcagt gcgggaggtg
     7861 ggaaatgatt cagtatctga acgtcttttt ttacgatatc tacccgtaca tttgtgcgac
     7921 ggtgtttttc ctcggcagct ggctgcgcta cgactacggg cagtacacct ggcgcgcctc
     7981 ctcaagtcaa atgctcgata aacgcgggat ggtgatatgg tcgaatctgt tccatatcgg
     8041 cattttgggg attttcttcg ggcacttgtt cggcatgtta acgccgcact ggatgtacgc
     8101 gtggttttta ccagtggcag cgaaacaact catggcgatg gtgctcggcg gtatttgcgg
     8161 tgtgttgacg ttgattggcg gcgcaggact gctgtggcgc aggctgacaa atcagcgcgt
     8221 gcgtgccact tccactacgc cggatatcat catcatgagc attttgctga tccagtgtct
     8281 gttggggcta agtaccatac cgttttcagc acagtatcct gatggtagcg aaatgatgaa
     8341 gctggtgggt tgggcgcaaa gcattgtcac tttccgtggt ggatcgtcag agatgctcaa
     8401 cggcgtagcg ttcgtcttcc gcctgcatct ggtgttggga atgaccatct tcctgctctt
     8461 cccgttcacc cgactggtgc atgtgtggag cgcgccgttt gagtacttta ctcgtcgata
     8521 tcaaattgtg cgttcgcggc gataatttca ccctggccgg atagcccttc gctctccggc
     8581 cacttgcagg ctatgatagt tctccagtca caggagaacg ttaatgaaac cgcaggtgta
     8641 ccacgtcgat gcttttacct cacaaccgtt tcgcggcaat tctgccggtg ttgttttccc
     8701 cgccgataat cttagcgaag cgcaaatgca gcttatcgcc cgcgagttag gccattcgga
     8761 aactgctttt ctgctgcaca gcgacgacag cgatgtgcgc atccgctact ttacgccaac
     8821 ggttgaagtg ccgatttgcg gtcacgcgac agtagctgcg cactatgtac gtgcgaaggt
     8881 gttaggttta ggaaattgca cgatctggca aacatcgctg gcaggaaaac atcgcgtgac
     8941 tatcgaaaag cacaacgatg attatcgtat ttcgctggaa caaggtacgc cgggctttga
     9001 actgccgctg gagggtgaag tacgcgcggc gattatcaac gcattgcatc ttactgaaga
     9061 cgatattctg ccgggtttgc cgatccaggt cgcgacgacc gggcattcta aagtgatgat
     9121 cccattgaag ccggaagtgg atatcgacgc tctttcgcct gaccttaatg cgctgaccgc
     9181 tatcagtaaa cagattggct gcaatggttt cttcccgttc cagatccgcc caggcaaaaa
     9241 cgaaaccgat ggtcgcatgt tctcgcctgc gattggtatt gtggaagatc cggtgactgg
     9301 caatgccaat ggcccgatgg gtgcatggtt ggtacatcac aacgtattgc cccacgatgg
     9361 caaggtgctg cgtgttaaag gccatcaggg gcgcgcattg gggcgtgacg gcgtgattga
     9421 agtgacggtg acaattcgtg ataaccaacc ggaaaaagtc accatttctg gcgcggcggt
     9481 gattttgttt catgccgaat gggcaattaa actctgaaac cgttgcctga tgcgcttcgc
     9541 ttatcaggtc tacaaccgag ctaatgtatg ccggataagg cgtttacgcc gcatccggca
     9601 acctgacata aattattttc ccgcctccgg gtgcgtatca aaagccgcca tcaccagcgc
     9661 taactcatcc acggtaaagc catgtttcgc atcatccacg cccagaccaa actgttcttg
     9721 catcacagca tataatgacg ccacatccgg tagatttcgc tgctccaccg cgtgcccatt
     9781 ttcataatgg gtaaaatgaa aattggtcag tgtcagcttg ccgccgtccg gcaaatggcg
     9841 gcacatcagc aaatgatggc gaaaatgcga ctgcggccag tgcgccgacc agaaattgcc
     9901 catcacataa tcgctttgtt gctgctcgca gagatcaaaa cggtacatcg actgccagtg
     9961 ctgatgatga ttaaactgca acacccaatc atcaccctcc tgcaacaacc gataatctcc
    10021 gtgtggcgtg gtctgcacga gatcggaaac taaacgaatc ggcgcggtta gcgtctgccc
    10081 accgaaaccg acatcagcaa tccatttttc ctcttccagt tccaccaaca gcaaacgatg
    10141 ggtgcgcggc ggtaatgctg gcggatttga taacactacg cgccctaaca agctgcgaac
    10201 gttaaactcc agctcgcgta acacccgctc aaacacgcca ttctgctcaa aacagtaacc
    10261 gccacgatgg gctatcacca gtttctcttc cagcgattga tcatcaagct gtatttccct
    10321 cggcagcaaa acgtcgaggt tttcaaacgg aatagtgcaa ttgtgtttca ggtgcaatgc
    10381 acgaagcgta tcaatattga ccgcagcagc tcccgaccag ttaatacggg caaaatagtg
    10441 attcagaatg ggcgtcatca ggcgtttccc ttagtggttt tctcaactta taacctggaa
    10501 tagacgccgc accggctatt ttgtgcgatc gcggacatgc atcacgtttg caaatcgcca
    10561 atcccgttaa tttcgttact tattgcgtta cggactacgc ttaaaaaaac cttcttcgcg
    10621 aggcgatacc atgagccgat tcatccccat agaattacac catgccagcc gtctgttaaa
    10681 tcatggccca actgtcatga tcaccagttt cgatgagcaa tcccagcggc gtaacattat
    10741 ggcagcagcc tggtcaatgc cggtggagtt tgaaccgcca cgcgtggcga ttgtggtaga
    10801 taaatcgaca tggaccaggg agttgattga acgtaacggt aaatttggca tcgttatccc
    10861 gggcgttgca gcaactaact ggacgtgggc agtgggaagc gtgtcgggtc gtgaagaaga
    10921 taaatttaat tgctatggca ttccggtggt gaaaggtccg gtgcttggtt tgccacttgt
    10981 tgaagagaaa tgcctggcgt ggatggagtg ccgcctgcta cctgcgactt ctgcgcaaca
    11041 aaaatacgac acgctgtttg gcgaggtggt atcagcagcg gcagacgcac gggtatttgt
    11101 cgaaggccgc tggcagtttg atgatgataa gctcaatacg ttgcatcatt taggtgctgg
    11161 cacgtttgtt accagcggca agcgagttac ggcgggttaa gcattacatg ctgtaaccag
    11221 gtttctttat caaagcctcc atttggggcg cgatttcggc atcccagata gcttgccaag
    11281 attctggttg aatctgctgt agagcaatgc ttatcgaact gtctttactg ttcagatggc
    11341 gaataataac gtcggtaata tctgcagcaa gtgctgcttt ttgttgttcg tccagttcac
    11401 gcggaaaaca tttaatgtcg atgtgcggca tgattgtgct ccttttgaaa gatgtcagta
    11461 aaattaacac acattgtgaa ttttcagtcc tttccattca aacgtgaaat caataagttc
    11521 atcagggaca tcgcaaacaa tatgaatacg gatttcttct ctgccgtgac tcttttcaca
    11581 aattgcgtaa ctgtcatgct ctggattatt taattctttc agcggaaatt tttcctcaaa
    11641 ggctttattt agccacccct ggtttccttt caccgcgaat aaataatcac ctccctgttt
    11701 ttgtatcttc tctgctatat ctttctggca acccatcgca tcagttgtga tgatttttcg
    11761 tttaatatcc agcatgttaa gaagttctgg gatagctgta atctcattag atttatcatc
    11821 cgtc
//
LOCUS       DBANUU010000133        11443 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000133 DBANUU010000000
VERSION     DBANUU010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.134"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            248..547
                     /gene="yoaC"
                     /locus_tag="ACIE68_003740"
     CDS             248..547
                     /gene="yoaC"
                     /locus_tag="ACIE68_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFX5909992.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(667..846)
                     /gene="yoaH"
                     /locus_tag="ACIE68_003741"
     CDS             complement(667..846)
                     /gene="yoaH"
                     /locus_tag="ACIE68_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFX5909993.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            920..2281
                     /gene="pabB"
                     /locus_tag="ACIE68_003742"
     CDS             920..2281
                     /gene="pabB"
                     /locus_tag="ACIE68_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFX5909994.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2285..2863
                     /gene="nudL"
                     /locus_tag="ACIE68_003743"
     CDS             2285..2863
                     /gene="nudL"
                     /locus_tag="ACIE68_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFX5909995.1"
                     /translation="MEYRSLTLDYFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3047..4411
                     /gene="sdaA"
                     /locus_tag="ACIE68_003744"
     CDS             3047..4411
                     /gene="sdaA"
                     /locus_tag="ACIE68_003744"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFX5909996.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMCFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4542..6140
                     /gene="pdeD"
                     /locus_tag="ACIE68_003745"
     CDS             4542..6140
                     /gene="pdeD"
                     /locus_tag="ACIE68_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFX5909997.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6144..7706)
                     /gene="yoaE"
                     /locus_tag="ACIE68_003746"
     CDS             complement(6144..7706)
                     /gene="yoaE"
                     /locus_tag="ACIE68_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFX5909998.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PIPEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHS
                     LFPVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRR
                     ARGSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHA
                     LQQALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVR
                     IVKEQPAHDEDE"
     gene            complement(7694..7852)
                     /gene="yoaL"
                     /locus_tag="ACIE68_003747"
     CDS             complement(7694..7852)
                     /gene="yoaL"
                     /locus_tag="ACIE68_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFX5909999.1"
                     /translation="MDRHRRHFSIRPFNAYLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8169..9140
                     /gene="manX"
                     /locus_tag="ACIE68_003748"
     CDS             8169..9140
                     /gene="manX"
                     /locus_tag="ACIE68_003748"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFX5910000.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9203..10003
                     /gene="manY"
                     /locus_tag="ACIE68_003749"
     CDS             9203..10003
                     /gene="manY"
                     /locus_tag="ACIE68_003749"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFX5910001.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10016..10867
                     /gene="manZ"
                     /locus_tag="ACIE68_003750"
     CDS             10016..10867
                     /gene="manZ"
                     /locus_tag="ACIE68_003750"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFX5910002.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10922..11380
                     /gene="yobD"
                     /locus_tag="ACIE68_003751"
     CDS             10922..11380
                     /gene="yobD"
                     /locus_tag="ACIE68_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFX5910003.1"
                     /translation="MTITDLVLILFIAVLLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2802 a         2875 c         2881 g         2885 t
ORIGIN      
        1 gttgtgttcg ccctctgact agcaactctg gttttctttc ttagctgaat aatctggtta
       61 tagcgtgagg actgaccgtt aaatttctcc atcttactgg ataacacacc atgagctgct
      121 acctttaaaa tacctgaatg tcaacaggtt aactcgtgcc atatcgttat aatccctttg
      181 atattagatg caaattaagg tcatatatag tcttatcgat aacatggtta attttaagga
      241 taagaatatg cctgctgtaa tagataaagc cctggatttc attggtgcca tggatgtatc
      301 agcgccaaca ccaagttcga tgaatgaaag cacggcgaag ggaatcttta aatatttaaa
      361 agaactggga gtacccgcga gtgccgctga tattacggcg cgagctgacc aggaaggctg
      421 gaaccctggg ttcacggaaa aaatggttgg atgggcaaaa aaaatggaga caggtgaacg
      481 ctctgtgatt aaaaatcctg aatacttttc aacatatatg caggaagaac taaaagcact
      541 ggtctgagtt aaatttatat cagcataaat gggtcaggac gcttttaatc acatataaaa
      601 agcgtccttt ttctcacaat caaatgatca tcggcttcta taacgaaatc gatacggatt
      661 agtgtgttac tcgtcttcat cctcaaaacg cgccacgatt cgctcgccgg agtggttggc
      721 acgcagctct tctgccacca gcgcgattgc ctggccgctg ctcatccctt gtgccatcag
      781 ttcctggatc cgctcgacag ctttttgctg ctgttcatgg gtgagtgaag gtaaacctgc
      841 aaacattgtt aactcctgct aaattgttgg cgctaattat ttcatgctac ccggcacata
      901 gccagtagag tcaggactga tgaagacgtt atctcccgct gtgattactt taccctggcg
      961 tcaggacgcc gctgaatttt atttctcccg cttaagccac ctgccgtggg cgatgctttt
     1021 acactccggc tatgccgatc atccgtatag ccgctttgat attgtggtcg ccgatccgat
     1081 ttgcacttta accactttcg gtaaagaaac cgttgttagt gaaagcgaaa aacgcacaac
     1141 gaccactgat gacccgctac aggtgctcca gcaggtgctg gatcgcgcag acattcgccc
     1201 aacgcataac gaagatttgc catttcaggg cggcgcactg gggttgtttg gctacgatct
     1261 gggccgccgt tttgagtcac tgccagaaat tgcggaacaa gatatcgttc tgccggatat
     1321 ggcagtgggt atctacgatt gggcgctcat tgtcgaccac cagcgtcata cagtttcttt
     1381 gctgagtcat aatgatgtca atgcccgtcg ggcctggctg gaaagccagc aattctcgcc
     1441 gcaggaagat ttcacgctca cttccgactg gcaatccaat atgacccgcg agcagtacgg
     1501 cgaaaaattt cgccaggtac aggaatatct gcacagcggt gattgctatc aggtgaatct
     1561 cgcccagcgt tttcatgcga cctattctgg cgatgaatgg caggcattcc ttcagcttaa
     1621 tcaggccaac cgcgcgccat ttagcgcttt tttacgtctt gaacagggtg caattttaag
     1681 cctttcgcca gagcggttta ttctttgtga taatagtgaa atccagaccc gcccgattaa
     1741 aggcacgcta ccacgcctgc ccgatcctca ggaagatagc aaacaagcag aaaaactggc
     1801 gaactcagcg aaagatcgtg ccgaaaatct gatgattgtc gatttaatgc gtaatgatat
     1861 cggtcgtgtt gccgtagccg gttcggtaaa agtaccagag ctgttcgtgg tggaaccctt
     1921 ccctgccgtg catcatctgg tcagcactat aacggcgcga ctaccagaac agttacacgc
     1981 cagcgatctg ctgcgcgcag cttttcctgg tggctcaata accggggctc cgaaagtacg
     2041 ggctatggaa attatcgacg aactggaacc gcagcgacgt aatgcctggt gcggcagcat
     2101 tggctatttg agcttttgcg gcaacatgga taccagcatt actatccgca cgctgactgc
     2161 cattaacgga caaatatact gctctgcggg cggtggaatt gtcgccgata gccaggaaga
     2221 agcggaatat caggaaactt ttgataaagt taataagata ttacgccaac tggagaagta
     2281 agacgtggaa taccgtagcc tgacgcttga ttatttttta tctcgctttc aacttttgcg
     2341 cccgcaaatt aaccgggaaa ccctaaatca tcgtcaggct gccgtgttaa tccccatcgt
     2401 ccgtcgaccg caaccggggt tgttgctgac tcagcgttca attcatctgc gtaaacacgc
     2461 tggacaagtg gcattccctg gaggtgcagt cgatgacacg gacgcatcag ttatcgccgc
     2521 cgcgctgcgc gaagctgaag aagaggtcgc tataccgcct tccgccgttg aagttatcgg
     2581 cgtgctgccg cccgtcgata gcgtaactgg ctatcaggta accccagtgg tcggcattat
     2641 cccgcccgat ctgccgtatc gcgccagtga agatgaagtc tcggcggtgt ttgaaatgcc
     2701 gctcgcccag gcattacatc tgggtcgtta tcacccttta gatatctacc gccgtggcga
     2761 ttcacatcgg gtatggctgt cctggtacga acagtatttt gtatggggaa tgaccgcagg
     2821 cataattcgt gagctggcgc tgcaaattgg tgtgaaaccc tgactatact tatctttaca
     2881 tctacaaaac actacttgag acaatcatcg caatattagt taaatcgcgg tttttgatta
     2941 gtttaattca tgtgaatagt taagccagtc gccgcgttcc ctcttacact atgcgctgtt
     3001 attagttcgt tactggaagt ccagtcacct tgtcaggagt attatcgtga ttagtctatt
     3061 cgacatgttt aaggtgggga ttggtccctc atcttcccat accgtagggc ctatgaaggc
     3121 gggtaaacag ttcgtcgatg atctggtcga aaaaggctta ctggatagcg ttactcgcgt
     3181 tgccgtggac gtttatggtt cactgtcgct gacgggtaaa ggccaccaca ccgatatcgc
     3241 cattattatg ggtcttgcag gtaacgaacc tgccaccgtg gatatcgaca gtattcccgg
     3301 ttttattcgc gacgtagaag agcgcgaacg tctgctgctg gcacagggac ggcatgaagt
     3361 ggatttcccg cgcgacaacg ggatgtgttt tcataacggc aacctgccgc tgcatgaaaa
     3421 cggtatgcaa atccacgcct ataacggcga tgaagtcgtc tacagcaaaa cttattattc
     3481 catcggcggc ggttttatcg tcgatgaaga acactttggt caggatgctg ccaacgaagt
     3541 gagcgtgccg tatccgttca aatctgccac cgaactgctc gcgtactgta atgaaaccgg
     3601 ctattcgctg tctggtctcg ctatgcagaa cgaactggcg ctgcacagca agaaagagat
     3661 cgacgagtat ttcgcgcatg tctggcaaac catgcaggca tgtatcgatc gcgggatgaa
     3721 caccgaaggt gtactgccag gcccgctgcg cgtgccacgt cgtgcgtctg ccctgcgccg
     3781 gatgctggtt tccagcgata aactgtctaa cgatccgatg aatgtcattg actgggtaaa
     3841 catgtttgcg ctggcagtta acgaagaaaa cgccgccggt ggtcgtgtgg taactgcgcc
     3901 aaccaacggt gcctgcggta tcgttccggc agtgctggct tactatgacc actttattga
     3961 atcggtcagc ccggacatct atacccgtta ctttatggca gcgggcgcga ttggtgcatt
     4021 gtataaaatg aacgcctcta tttccggtgc ggaagttggt tgccagggcg aagtgggtgt
     4081 tgcctgttca atggctgctg cgggtcttgc agaactgctg ggcggtagcc cggaacaggt
     4141 ttgcgtggcg gcggaaattg gcatggaaca caaccttggt ttaacctgcg acccggttgc
     4201 agggcaggtt caggtgccgt gcattgagcg taatgccatt gcctctgtga aggcgattaa
     4261 cgccgcacgg atggctctgc gccgcaccag tgcaccgcgc gtctcgctgg ataaggtcat
     4321 cgaaacgatg tacgaaaccg gtaaggacat gaacgccaaa taccgcgaaa cctcacgcgg
     4381 tggtctggca atcaaagtcc agtgtgacta atacttctta ctcgcccatc tgcaacggat
     4441 gggcgaattt atacccgctt tctcgtctgc tgtaatattc cccactacac ttccactgtt
     4501 gcgtcaggcg tttgtcgcca tacgcttaca gggtggcccg catgcaaaaa gcacaacgga
     4561 tcattaaaac ctatcgccgt aatcgaatga ttgtttgtac gatttgcgca ctcgttacgc
     4621 tcgcttcgac cctgagcgtg cgatttattt cacagcgtaa cttaaatcaa caacgggtag
     4681 tacaattcgc caatcacgct gtagaggaat tagataaagt actgcttccc ctacaggcag
     4741 gtagcgaagt cttgcttccg ctgattggtc tgccctgctc tgtcgcccat ttgccattac
     4801 gtaaacaggc ggcaaaactc caaactgtgc gatccattgg cctggtgcaa gacggcacac
     4861 tttattgctc cagcattttt ggttatcgca atgtgcccgt cgtggacatt ctggctgaac
     4921 ttcctgcacc gcaaccactt ttacgcctga cgatcgaccg tgccctgatt aaaggcagtc
     4981 cggttttgat tcaatggacg cctgcagcgg gcagtagcaa tgctggggtc atggagatga
     5041 ttaacatcga cttactgacg gcaatgctcc ttgagccaca actgccgcaa atcagtagcg
     5101 ccagcctgac ggtggacaaa cggcatttgc tctatggtaa tgggctggta gattcccttc
     5161 cgcaacctga agacaatgaa aactatcagg tttcttcgca acgctttcct tttaccatta
     5221 acgttaatgg tccgggggct acggcgctgg catggcacta tcttccaaca caattaccgc
     5281 tggcggtgct gctaagttta ctggtgggct acatcgcctg gctggcgacc gcttaccgga
     5341 tgagcttttc ccgcgaaatc aatctgggcc tggcgcaaca tgagttcgaa ttgttctgtc
     5401 agcctttgct taatgcgcgc agccagcaat gtattggtgt agagattttg ctgcgctgga
     5461 acaatccgcg tcagggctgg atttcaccgg atgtgtttat tcctatcgcg gaagaacatc
     5521 atttaattgt gccactgacc cgctatgtga tggcagaaac cattcgtcag cgccatgttt
     5581 tcccgatgag tagtcagttt catgttggca ttaacgtcgc acccagccat tttcgccgtg
     5641 gtgtgctgat aaaagatctc aatcagtact ggtttagcgc tcacccgatt cagcaactga
     5701 tcctcgaaat caccgaacgc gatgccttac tggatgttga ttatcggatt gcccgcgagc
     5761 tacatcgtaa aaacgtcaaa ctggcgattg atgacttcgg caccggcaac agttcatttt
     5821 cctggcttga aacattacgt cctgacgtgc tgaaaattga taagtcattt accgcagcta
     5881 taggttctga cgcggttaac tcgacggtga ccgatatcat catcgcgctg gggcaaagac
     5941 tgaatattga actggtggcg gagggcgtgg aaacgcaaga acaggcgaag tatttgcgcc
     6001 gtcatggcgt gcatattttg caagggtatt tgtacgcaca gccgatgccg ctacgtgatt
     6061 ttcccaaatg gctggcgggc agccaaccgc cgcccgcccg gcataatgga catatcacgc
     6121 ccattatgcc gttacgttaa tgcttactca tcttcatcgt gcgccggttg ctctttaaca
     6181 atgcgaacca gatcaacacg ataatcattg gcttcaatga tggtgatatg cagtggccct
     6241 acatcaatca catcgcccac acggggaatg tgaccatttg ccgagatcac gaggcccgcg
     6301 accgtcgcga tatcatcgtc atcggcaagg tgctcaacat caagcgcctg ctgcaaggca
     6361 tgcaaatctg taccgccttt taccagccag ccgtcaccat cagtaatgat ttccggcgtt
     6421 tcgtcagcgt ccgggaattc acccgcaatg gcttccagca catccagcgg cgtgaccaga
     6481 ccttgtacca caccaaactc gttggtcacg ataacaaagc tcccgcgagc acgacgcagc
     6541 acgcccaaca ggttgatcgg atcgagggtt tccgggacga taatcgccgg agacgccgaa
     6601 gcaatcgccg ccacatcaac gccctcttcc agcgccacca gcagttcttt agcacgtaca
     6661 ataccgatga tttcatccag ttcaccgcga cataccggga acagactgtg cggtgaagag
     6721 agcagttgct cgcggatttc atcgaccccg agattagcgt caacccagct tatttcaccg
     6781 cgcggcgtca tgatcccgcg cagagaacgc gacgccagcg tcagtacgcc gttaatcatg
     6841 taacgttctt cttcggcaaa tgcaccttcc gggatcggga tcggcatcgg catcgggtta
     6901 tcggcatcgt gctgaacatt ggcctgacgt ttcccgccca tcaaacgcag gatggcatcg
     6961 gcagtacgcg ctcgcagcgg caaagtcgac tggtggcgaa taaagttgcg acgcgcaatc
     7021 tggttaaaca cttcgatgat gatcgagaag ccaatcgcgg catacaggta acctttcgga
     7081 atgtggaaac cgaaaccttc tgccaccaga ctcagaccaa tcattaacag gaagctcaga
     7141 cagagcacca ccaccgtggg gtgctggtta acgaatcgcg tcagcggttt ggatgccagc
     7201 aacataaccg ccatcgcaat cactaccgcc gccatcatca ccggcagatg gttaaccatc
     7261 cctactgcag taattaccgc atccaacgag aagacggcgt caaggatgac aatctgtgtg
     7321 acgaccaccc agaaactggc gtagccttta ccgtggccgg aatcatgatc gcggttttcc
     7381 agccgttcat gcagttcggt tgttgctttg aacagcaaga atatcccccc gaacaacata
     7441 atcaggtcgc gtccggagaa ggagaaatcc atgacggtaa atagcggttt ggtcagcgtg
     7501 accatccatg aaatcagcga cagcagcccc agacgcataa tcagcgccag tgataacccc
     7561 agcaaacgcg ctttatcgcg ttgttttggc ggcagtttgt cagcaagaat ggcgatgaag
     7621 accaggttat cgatacccag cacaatttcg agaacaacaa gcgtgagtag ccccgcccaa
     7681 attgaggggt ccattaagaa ttccatgaca agctcctgct taaggaatag ctattcgacg
     7741 ccagaaataa tgcaggcgta acgacaaaat gcaaacgaaa ggtgcggcat agagagccag
     7801 aaaggtaggc gttaaaaggc ctgatgctga aatgacgtcg gtgacgatcc atactgcggg
     7861 ctactgccct atactccatg gttgttaaac gggagttaaa catatcagag acgcctctga
     7921 tttggcaaag atttaccttc ctttgcaaac gaatgtgaca aggatatttt acctttcgaa
     7981 atttctgcta atcgaaagtt aaattacgga tcttcatcac ataaaataat tttttcgata
     8041 tctaaaataa atcgcgaaac gcaggggttt ttggttgtag cccttatctg aatcgattcg
     8101 attgtggacg acgattcaaa aatacatctg gcacgttgag gtgttaacga taataaagga
     8161 ggtagcaagt gaccattgct attgttatag gcacacatgg ttgggctgca gagcagttgc
     8221 ttaaaacggc agaaatgctg ttaggcgagc aggaaaacgt cggctggatc gatttcgttc
     8281 caggtgaaaa tgccgaaacg ctgattgaaa agtacaacgc tcagttggca aaactcgaca
     8341 ccactaaagg cgtgctgttt ctcgttgata catggggagg cagcccgttc aatgctgcca
     8401 gccgcattgt cgtcgacaaa gagcattatg aagtcattgc aggcgttaac attccaatgc
     8461 tcgtggaaac gttaatggct cgtgatgatg acccaagctt tgatgaactg gttgcgctgg
     8521 cagtagaaac aggccgtgaa ggcgtgaaag cactgaaagc caaaccggtt gaaaaagccg
     8581 cgccagcacc cgctgccgca gcaccaaaag cggctccaac tccggcaaaa ccaatggggc
     8641 caaacgacta catggttatt ggccttgcgc gtatcgacga ccgtctgatt cacggtcagg
     8701 tcgccacccg ctggaccaaa gaaaccaatg tctcccgtat tattgttgtt agtgatgaag
     8761 tggctgcgga taccgttcgt aagacactgc tcacccaggt tgcacctccg ggcgtaacag
     8821 cacacgtagt tgatgttgcc aaaatgattc gcgtctacaa caacccgaaa tatgctggcg
     8881 aacgtgtaat gctgttattt accaacccaa cagatgtaga gcgtctcgtt gaaggcggcg
     8941 tgaaaatcac ctctgttaac gtcggtggta tggcattccg tcagggtaaa acccaggtga
     9001 ataacgcggt ttcggttgat gaaaaagata tcgaggcgtt caagaaactg aatgcgcgcg
     9061 gtattgagct ggaagtccgt aaggtttcca ccgatccgaa actgaaaatg atggatctga
     9121 tcagcaaaat cgataagtaa cgtattgtgt tgattatcac tcagttttca cacttaagtc
     9181 ttacgtaaac aggagaagta caatggagat taccactctt caaattgtgc tggtatttat
     9241 cgtagcctgt atcgcaggta tgggatcaat cctcgatgaa tttcagtttc accgtccgct
     9301 aatcgcgtgt accctggtgg gtatcgttct tggcgatatg aaaaccggta ttattatcgg
     9361 tggtacgctg gaaatgatcg cgctgggctg gatgaacatc ggtgctgcag ttgcgcctga
     9421 cgccgctctg gcttccatca tttctactat tctggttatc gcaggtcatc agagcattgg
     9481 tgcaggtatc gcactggcaa tccctctggc cgctgcgggc caggtactga ccatcatcgt
     9541 tcgtactatt accgttgctt tccagcacgc tgcggataag gctgctgata acggcaacct
     9601 gacagcgatt tcctggatcc acgtttcttc tctgttcctg caagcaatgc gtgtggctat
     9661 tccggccgtc atcgttgcgc tgtctgttgg taccagcgaa gtacagaaca tgctgaatgc
     9721 gattccggaa gtggtgacca atggtctgaa tatcgccggt ggcatgatcg tggtggttgg
     9781 ttatgcgatg gttatcaaca tgatgcgtgc tggctacctg atgccgttct tctacctcgg
     9841 cttcgtaacc gcagcattca ccaactttaa cctggttgct ctgggtgtga ttggtactgt
     9901 tatggcagtg ctctacatcc aacttagccc gaaatacaac cgcgtagccg gtgcgcctgc
     9961 tcaggcagct ggtaacaacg atctcgataa cgaactggac taacaggtga gcgaaatggt
    10021 tgatacaact caaactacca ccgagaaaaa actcactcaa agtgatattc gtggcgtctt
    10081 cctgcgttct aacctcttcc agggttcatg gaacttcgaa cgtatgcagg cactgggttt
    10141 ctgcttctct atggtaccgg caattcgtcg cctctaccct gagaacaacg aagctcgtaa
    10201 acaagctatt cgccgtcacc tggagttctt taacacccag ccgttcgtgg ctgcgccgat
    10261 tctcggcgta accctggcgc tggaagaaca gcgtgctaat ggcgcagaga tcgacgacgg
    10321 tgctatcaac ggtatcaaag tcggtttgat ggggccgctg gctggtgtag gcgacccgat
    10381 cttctgggga accgtacgtc cggtatttgc agcactgggt gccggtatcg cgatgagcgg
    10441 cagcctgtta ggtccgctgc tgttcttcat cctgtttaac ctggtgcgtc tggcaacccg
    10501 ttactacggc gtagcgtatg gttactccaa aggtatcgat atcgttaaag atatgggtgg
    10561 tggcttcctg caaaaactga cggaaggggc gtctatcctc ggcctgtttg tcatgggggc
    10621 attggttaac aagtggacac atgtcaacat cccgctggtt gtctctcgca ttactgacca
    10681 gacgggcaaa gaacacgtta ctactgtcca gactattctg gaccagttaa tgccaggcct
    10741 ggtaccactg ctgctgacct ttgcttgtat gtggctactg cgcaaaaaag ttaacccgct
    10801 gtggatcatc gttggcttct tcgtcatcgg tatcgctggt tacgcttgcg gcctgctggg
    10861 actgtaagac tgttgtacac taccggggcc ttttggcccc gtttttttat ctggaggatt
    10921 aatgacaatc acggacctgg tactgattct tttcatcgcc gtactcctgg ccttcgcgat
    10981 ctacgatcag ttcatcatgc cccgccgtaa tggccccacc ctgctggcaa ttcctttgct
    11041 ccggcgtggt cgcatcgata gcgttatctt cgtcggattg attgtcattc ttatctataa
    11101 caacgtcacg aatcatggtg cgttaataac gacatggtta ttaagcgcac tggctctgat
    11161 gggtttttat atattctgga tccgcgttcc gaagatcatc tttaaacaaa aaggtttttt
    11221 cttcgccaat gtctggattg aatatagccg aatcaaagcg atgaacttgt cggaagatgg
    11281 cgtgttggtg atgcaattag aacagcgtcg gctgttaatc cgcgttcgaa atatcgacga
    11341 tctggaaaaa atttataagc ttctcgtttc aactcaataa gttatgaatt tagccaaagc
    11401 tatgtttagt gtatttttaa taatcagaca tagcttaggc tat
//
LOCUS       DBANUU010000134        11427 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000134 DBANUU010000000
VERSION     DBANUU010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.135"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            295..1161
                     /gene="folD"
                     /locus_tag="ACIE68_003752"
     CDS             295..1161
                     /gene="folD"
                     /locus_tag="ACIE68_003752"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFX5910004.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            1163..1375
                     /gene="ybcJ"
                     /locus_tag="ACIE68_003753"
     CDS             1163..1375
                     /gene="ybcJ"
                     /locus_tag="ACIE68_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFX5910005.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1483..2004
                     /locus_tag="ACIE68_003754"
     CDS             1483..2004
                     /locus_tag="ACIE68_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFX5910006.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FNFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(2040..3425)
                     /gene="cysS"
                     /locus_tag="ACIE68_003755"
     CDS             complement(2040..3425)
                     /gene="cysS"
                     /locus_tag="ACIE68_003755"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFX5910007.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3599..4093
                     /gene="ppiB"
                     /locus_tag="ACIE68_003756"
     CDS             3599..4093
                     /gene="ppiB"
                     /locus_tag="ACIE68_003756"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFX5910008.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4096..4818
                     /gene="lpxH"
                     /locus_tag="ACIE68_003757"
     CDS             4096..4818
                     /gene="lpxH"
                     /locus_tag="ACIE68_003757"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFX5910009.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQGLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4936..5445
                     /gene="purE"
                     /locus_tag="ACIE68_003758"
     CDS             4936..5445
                     /gene="purE"
                     /locus_tag="ACIE68_003758"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFX5910010.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5442..6509
                     /gene="purK"
                     /locus_tag="ACIE68_003759"
     CDS             5442..6509
                     /gene="purK"
                     /locus_tag="ACIE68_003759"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFX5910011.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(6648..7541)
                     /locus_tag="ACIE68_003760"
     CDS             complement(6648..7541)
                     /locus_tag="ACIE68_003760"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFX5910012.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7538..8353)
                     /locus_tag="ACIE68_003761"
     CDS             complement(7538..8353)
                     /locus_tag="ACIE68_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFX5910013.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWMLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRQFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8364..9623)
                     /locus_tag="ACIE68_003762"
     CDS             complement(8364..9623)
                     /locus_tag="ACIE68_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFX5910014.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWVKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9633..11300)
                     /locus_tag="ACIE68_003763"
     CDS             complement(9633..11300)
                     /locus_tag="ACIE68_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFX5910015.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGAGSSQAL
                     TQVRRWDSACQKLPDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
BASE COUNT         2555 a         3254 c         3012 g         2606 t
ORIGIN      
        1 attcaacaca aatatagacc tgaagcggta aattaccagg ctgaaaattc tttttatatt
       61 gtcaggtatt tcttaaatta tcttaatcct tagacaagga aataaatcag ttccagattt
      121 acaacgccat catggacgaa aaatgaagct ttcagtctca gcgacggtgc gcctcacctt
      181 cgcaagaggt cgcttcacgc gataaatctg aaacgaaacc tgacagcgcg ccccgcttct
      241 gacaaaatag gcgcatcccc ttcgacctac gtaacagatg gaatcctctc tctgatggca
      301 gcaaagatta ttgacggtaa aacgattgcg cagcaggtgc gctctgaagt tgctcaaaaa
      361 gttcaggcgc gtattgcagc cggactgcgg gcaccaggac tggccgttgt gctggtgggt
      421 agtaaccctg catcgcaaat ttatgtcgca agcaaacgca aggcttgtga agaagtcggg
      481 ttcgtctccc gctcttatga cctcccggaa accaccagcg aagcggagct gctggagctt
      541 atcgatacgc tgaatgccga caacaccatc gatggcattc tggttcaact gccgttaccg
      601 gcgggtattg ataacgtcaa agtgctggaa cgtattcatc cggacaaaga cgtggacggt
      661 ttccatcctt acaacgtcgg tcgtctgtgc cagcgcgcgc cgcgtctgcg tccctgcacc
      721 ccgcgcggta tcgtcacgct gcttgagcgt tacaacattg ataccttcgg cctcaacgcc
      781 gtggtgattg gcgcatcgaa tatcgttggc cgcccgatga gcatggaact gctgctggca
      841 ggttgcacca ctacagtgac tcaccgcttc actaaaaatc tgcgtcatca cgtagaaaat
      901 gccgatctat tgatcgttgc cgttggcaag ccaggcttta ttcccggtga ctggatcaaa
      961 gaaggcgcaa ttgtgattga tgtcggcatc aaccgtctgg aaaatggcaa agttgtgggc
     1021 gacgtcgtgt ttgaagacgc ggctaaacgc gcctcataca ttacgcctgt tcccggcggc
     1081 gttggcccga tgacggttgc cacgctgatt gaaaacacgc tacaggcgtg cgttgaatat
     1141 catgatccac aggatgagta acatggcgac attttcttta ggtaaacatc cgcacgttga
     1201 gctgtgcgac ttgctgaaac tggaaggctg gagcgaaagc ggcgcgcagg cgaaaatcgc
     1261 gattgccgaa ggccaggtga aagtcgacgg tgcggttgaa acgcgcaaac gctgcaaaat
     1321 cgtcgccggt cagacagtga gttttgcagg tcacagcgta caggttgttg cctgatccct
     1381 ttcccggcct gtatcaacag gccggttctc ttttcagact tttgccctct acgccccgtt
     1441 catccatgct gatagcatcg acattttgcc gaagcgacca tcatgccaac cgttattacg
     1501 cacgccgccg ttcccctatg cattggttta gggttgggtt cgaaagtcat ccctccgcgc
     1561 ctgttatttg ccggaatcat cctggcgatg ctgcccgatg ccgacgtctt gtcgtttaat
     1621 tttggcgttg cttacggcaa tgtttttggt catcgcgggt ttacccattc gctggtgttt
     1681 gcgtttgttg tcccgctgtt atgtgtgctg attggacgtc gatggttcag ggctgggctg
     1741 attcgctgct ggctgttttt aaccgtctcg ttgctatcgc acagcttgct ggattcggta
     1801 accactggcg gtaaaggggt tggctggctg tggccgtggt cagatgaacg ctttttcgct
     1861 ccctggcagg tgattaaagt cgcgccgttt gcattgtctc gttacaccac agcgtacggg
     1921 catcaggtga ttatttctga attgatgtgg gtgtggttgc cggggatgct actgatggga
     1981 atgttgtggt ggcgcagacg ataaccggat gcgaaaactc gcatccggca atagcgcaat
     2041 tacttacgac gccaggtggt cccttgcggg ccatcttcca gcacgatccc catctcatta
     2101 agacggtcac gcgccgcatc tgccgccgcc cagtctttcg ctttacgggc atccagacgc
     2161 tgttgaatta acgcttcaat ctcagccact tcgctgtcgt ctgcctgcgc gccgctttgc
     2221 aggaacgctt ccggttcttg ctccagcagg cccaatacgg cggaaagttt acgcaggtga
     2281 gatgccattg cattcgccgc tgccatatct tctgctttca gacggtttac ttcacgcgcc
     2341 atatcaaaca gcacggaata ggcttccggg gtgttgaaat cgtcgtccat cgcttcaata
     2401 aagcgcgctt caaacgcttc gccaccggca ggcgcaacgg ttttatctgt gccgcgcagc
     2461 gcagtgtaga gacgctccag cgccgcacgc gcctgcttca ggttctcttc gctatagttc
     2521 agctggctgc gatagtggcc cgacatcagg aagtaacgca cggtttccgc gtcgtagtat
     2581 ttcagcacat cgcgcacggt aaagaagtta cccagcgatt tggacatctt ctcgcggtca
     2641 accatcacca tccccgagtg catccagtag ttcacatact gaccatcatg ggcacaggtg
     2701 gactgcgcga tttcgttttc gtggtgcggg aacatcaggt ctgaaccgcc gccgtggata
     2761 tcaaagtggt tacccagctg cttgcagttc attgccgaac attcaatgtg ccagccagga
     2821 cgacccgcgc cccacggaga cggccagctc ggttcgccct ctttcgacat cttccacaga
     2881 acgaagtcca ttgggttgcg tttgtcgtcg accacgtcaa cgcgcgcgcc tgcctgcagc
     2941 tggtcgagat cctgacgcga cagcacgcca taagttggat cggtcgggac gtcgaacatc
     3001 acgtcgccgt tgtccgccac ataagcgtga cctttggcga tcagttgttc agtgagttca
     3061 ataatttctg cgatatggtg cgtcgcgcgc ggctccatat ccgggcgcag aatgttcaac
     3121 gcatcaaaat ctttgtgcat ttcggcgatc atgcgatcca ccagcgccac aaagctttcg
     3181 ccattttcat tggcgcgttt gatgattttg tcgtcgatat cggtaatgtt gcgcacatac
     3241 ttcagcttat agccgaggaa acgcagatag cgcgcaacca cgtcaaaagc aacaaaggta
     3301 cgcccgtgac cgatatgaca gagatcgtaa acggtgattc cacacacgta catgccgact
     3361 tccccggcgt gaataggctt aaattcctct ttttggcgtg tcagagtatt gaagattttt
     3421 agcatcgaag attccgttta gacatgtgtg ggtaattgag ttgcgtataa tacccatatt
     3481 tcccgcgcga atcagcatac attgcgtgat gatccgatcg tgcggttatg ctataacacc
     3541 accctatata tgacccgaac tgggttgaag caccaatcaa acggaacagg atgcaaaaat
     3601 ggttactttc cacaccaatc acggcgatat tgtcatcaaa acttttgacg ataaagcacc
     3661 tgaaacagtt aaaaacttcc tggactactg ccgcgaaggt ttttacaaca acaccatttt
     3721 ccaccgtgtt atcaacggct ttatgattca gggcggcggt tttgaaccgg gcatgaaaca
     3781 aaaagccacc aaagaaccga tcaaaaacga agccaacaac ggcctgaaaa atacccgtgg
     3841 tacgctggca atggcacgta ctcaggctcc gcactctgca actgcacagt tcttcatcaa
     3901 cgtggttgat aacgacttcc tgaacttctc tggcgaaagc ctgcaaggtt ggggctactg
     3961 cgtgtttgct gaagtggttg acggcatgga cgtggtagac aaaatcaaag gtgttgcaac
     4021 cggtcgtagc ggtatgcacc aggacgtgcc aaaagaagac gttatcattg aaagcgtgac
     4081 cgttagcgag taatcgtggc gacactcttt attgcagatc ttcatctctg cgtggaagaa
     4141 ccggcgatca ccgccggttt tctgcgtttt ttagcggggg aagcccgcaa ggccgacgcg
     4201 ctgtatattc ttggcgatct gtttgaagca tggattggcg acgacgatcc caacccactc
     4261 catcgccaga tggcggcagc gatcaaagcg gtgtccgatt ccggcgttcc ctgttatttc
     4321 attcatggca accgtgattt tctgctcggc aaacgctttg cccgtgaaag cggcatgaca
     4381 ttattgccgg aagaaaaggt gctcgaactt tatggtcgcc gggtgttgat tatgcatggc
     4441 gacacgctgt gcaccgatga cgcgggttat caggcttttc gcgccaaagt ccacaaaccc
     4501 tggctgcaaa tgctattcct cgctctgccg ttgtttgtgc gcaaacgcat tgccgcgcga
     4561 atgcgcgcga acagtaaaga agccaatagc agtaaatcgc tggcgatcat ggacgttaac
     4621 caaaacgcgg tagtcagtgc gatggaaaaa catcaggtgc aagggctgat ccacgggcat
     4681 acccatcgcc cggcggtgca tgaacttatc gccaatcagc aacctgcttt tcgcgtggta
     4741 ctgggtgcct ggcatacgga aggttcgatg gtgaaagtca cggcggatga cgttgagcta
     4801 attcattttc cgttttaaaa aacccgcaac tttgctgatt tcacagccac gcaaccgttt
     4861 tccttgctct ctttccgtgc tattctctgt gccctctaaa gccgagagtt gtgcaccaca
     4921 ggagttttaa gacgcatgtc ttcccgcaat aatccggcgc gtgtcgccat cgtgatgggg
     4981 tccaaaagcg actgggctac catgcagttc gccgccgaaa tcttcgaaat cctgaatgtc
     5041 ccgcaccacg ttgaagtggt ttctgctcac cgcacccccg ataaactgtt cagcttcgcc
     5101 gaaagcgccg aagagaacgg ttatcaggtg attattgcgg gcgcaggcgg cgcagcgcat
     5161 ctgccaggca tgattgccgc caaaacgctg gtgccggtgc tgggcgtgcc agtacagagc
     5221 gccgcactga gcggtgtcga tagcctctac tccatcgtac aaatgccgcg cggcattccg
     5281 gtgggtacgc tggcgattgg taaagctggc gcggcaaacg cggcgttact ggcagcacaa
     5341 attcttgcga ctcatgataa agaactgcac cagcgtctga atgactggcg caaagcccag
     5401 accgacgaag tgctggaaaa cccggacccg cgaggtgcgg catgaaacag gtttgcgtcc
     5461 tcggtaacgg gcagttaggc cgtatgctgc gtcaggcagg cgaaccgtta ggcattgctg
     5521 tctggccagt cgggctggac gctgaaccgg cggcggtgcc ttttcaacaa agcgtgatta
     5581 ccgctgagat agaacgctgg ccggaaaccg cattaacccg cgagctggca cgccatccgg
     5641 cctttgtgaa ccgcgatgtg ttcccgatta ttgctgaccg tctgactcag aagcagcttt
     5701 tcgataagct ccacctgccg actgcaccgt ggcagttact tgccgaacgc agcgagtggc
     5761 ctgcggtgtt tgatcgttta ggtgagctgg cgattgttaa gcgtcgcact ggtggttatg
     5821 acggtcgcgg tcaatggcgt ttacgcgcaa atgaaaccga acagttaccg gcagagtgtt
     5881 acggcgaatg tattgtcgag cagggcatta acttctctgg tgaagtgtcg ctggttggcg
     5941 cgcgcggctt tgatggcagc accgtgtttt atccgctgac gcataacctg catcaggacg
     6001 gtattttgcg caccagcgtc gcttttccgc aggccaacgc acagcagcag gcgcaagccg
     6061 aagagatgct gtcggcgatt atgcaggagc tgggctatgt gggcgtgatg gcgatggagt
     6121 gttttgtcac cccgcaaggt ctgctgatca acgaactggc tccgcgtgtg cataacagcg
     6181 gtcactggac acaaaacggt gccagcatca gccagtttga gctgcatctg cgggcgatta
     6241 ccgatctgcc gttaccgcaa ccagtagtga atagtccgtc ggtgatgatc aatctgattg
     6301 gtagcgatgt gaattatgac tggctgaaat tgccgctggt gcatctgcac tggtacgaca
     6361 aagaagtccg tccggggcgt aaagtggggc atctgaattt gaccgacagc gacacatcgc
     6421 gtctgtccgc gacgctggaa gccttaatcc cgctgctgcc gccggaatat gccagcggcg
     6481 tgatgtgggc acagagtaag ttcagttaac ctggtgctca tttgattgcc ggatgcggcg
     6541 tgaacgcctt atccggccta caccgatccg gtacccattg taggcctgat aagacgcgtc
     6601 aagcgtcgca tcaggcatgg tgcaccgatt gctggatgct ctcatcacta cagcgaaata
     6661 caggtccccg cttcgcccgc cagcgtctct tcaattcgcg ataacgcccc aatccacgcg
     6721 ggtttaccac ggcttctgac ataaccactc accgccgtta ccttcggccc catcgaacca
     6781 tcggctttgg caaatggcgc taactcatcc ggtgtggcat ggcgaatggc gcgttgctgc
     6841 ggcgttcccc agttttcata taccgcatca gcatcagtga ggatcaccag cccatcggcg
     6901 ttaatctgct cagcgagcaa cgcagcagcg agatctttat caatcaccgc ttcactccct
     6961 gccccatctt ccgtcacagg cacaccgccg ccgccactgc aaatcaccac atgcccctct
     7021 ttgagcaaca actcgatggc ttcgctgtcg agaattttac gcggttgcgg agacgccacc
     7081 acccggcgca aatatttacc atcacgtttc atctgccagc cgtaagccgc ttccagtgcc
     7141 tcttgttctt ctggctgata aaccggacca ataaatttct ctggctgcaa aaacgccgga
     7201 tcatcaggcg aaacctcaat gcgcgtcagc accgtcgtca cgggcggcat ctgcggctgt
     7261 gcgctcaaac tctgcgccag catatagcca atcatcccct gactttctgc caccagcaca
     7321 tccagcggat acggttctac ctctttccac gccagattct gcaatgccag cagccccacc
     7381 tgcggcccgt tgccgtgaac aatcgccagc cgataagaac gggccaggcg cgccagcgcg
     7441 ggtacagcac tggcgatatt gcgatattga ttttctgccg tcagcgcctc accgcgctgg
     7501 agtaaggcgt tgcccccaag agctacaacc agtgttttca tggttttcct tgtaataatt
     7561 gttgaccaag ccagaacccc agcagtgtat ctgcccctga cgtatgcccc agcgccagca
     7621 gcgaatcaat cgcaacagcg gtacgtttcg ggcaactcag agcatgaaca aagtgcagga
     7681 gtggcgaggc gaaatatcct tgcgcggcat aacgtaaata actgacgctc accgctgtgg
     7741 taacgagttg cagattgtcg gaacaggcaa aaaactggcg accggagcgc gcatctaaag
     7801 cgccataata ccaggccgcc agcagcattc cgctcagcgt gtcatcatga ctcggcgtta
     7861 atccggggcc tttacccagc cagtgccgcc agtcggtctt aacgccattg agcgcggcct
     7921 gaaaacagtg acgaaactgg cgtaactcag caggcagcgg atcgcttgcc gccaacgcca
     7981 gtggcccgaa aagcccggtt tcctccgcgc gttgcatcca tgcagctgca agtggttgag
     8041 gatgcgcagg cggcgtaata cgcagcaaac aataacgctg tggctgttta accgtgaaac
     8101 gcccgaggcg aatcccttga gccacaacct gtggtcgttc attgccgcat aatccaccgc
     8161 ataacgcatc gaattgcgca cggcgaagca tccatcctcc ggggccgaaa ccactaccct
     8221 gacgatgcag cgttaacagt tcgccgcttt ccgtcatcag gttaatcgcc cgccgccaca
     8281 ctcccgctaa ccgccaggac tggcgataat tcggtgcgct actactggca agcagaggat
     8341 ggatgatcgt cattgcgcgt tcctcaacca atccccatgc tttcagccag tgcttccagc
     8401 gcctgttcaa aacacgccag cggtgcccgc acagtgcctg cgccaatctg cccgatcccc
     8461 gcctctttat gggcgatacc ggtattgatg agtggcgtaa tgccggtttc taccacgcga
     8521 cgaatgtcca gtcccaggca cgcgccctga aaatcccagc ctgggatctg caactgcata
     8581 ttgcgctcaa ggtaaatttc cgccatctct tcagataccg ctctggccgc ttccatgcca
     8641 cccgcaccga caaagcgcgt tacgccaggc gctgcgatca ttgccgcacc tccgatacca
     8701 aaggtttcgg taatcgcgct atcgcccata tccgggttcg cctgctcctg cgagaagccg
     8761 gtgaaaaaca gaccttgcgg agtgtttaca ggcgcagtaa accagcgttc gcccagcccg
     8821 cttacccgaa tcccgaacat attgccgttg cgggtcattg ccgtgacgat gctgcctgcg
     8881 cggatcatcg cgccagcatc catcgccgcc ttgcagtaag ccatcgcgag gttgaggaag
     8941 aactgatcgg tcacgctgag gaaatccatc acttcggcga tgtgctgttt atcatgatcg
     9001 aggcgagcaa tttgtggggc cagcgcacgc attaacagtg cagaggaagc aatattgcgt
     9061 tgatggaact catcgcccat cgtaatgccc tgcgccatca tcgccgtgag atcgataccg
     9121 cgctccatgc gccccagcgc cgcgcttaat actggcatta acacatcgcg catccagcga
     9181 tggcgagtca ggacatcttc gccgtaagcg ccaaaacgca tcactttgcc gataccttcg
     9241 ttgaggttgc agtacgcccg gttgccgtcg gtcacgttct cgaccaccag catcggcata
     9301 ctggcagaag taataccgcc cattggaccg acggcattca catggtgaca aggaatgaag
     9361 ttcacctccc cctgctccag tattgccagc gcctgcgctt catctttcac ccagccttcg
     9421 aacagacatg cgcccacgca cgcccctttc atgggtccgg tcatctcctg ccagcgcatt
     9481 ggcggcccgg cgtgaagcag tgttttgccc tcgtttagtt cgctgataag tgaagaagcc
     9541 ggttgcacat ccagccagtg tggacgagcg cgacgaattt gttcgataac cgcagcattg
     9601 gcttgcgcta ctgatgtaaa catgggaacc ccttattgca aacgttctaa taaacgagcc
     9661 agttttttat tgccgccggc gactggcgac cattggtaat gcacaaccgg tttgctggcg
     9721 ctttgtagct ccagcgcaaa gctgcgtaat ccgatgttaa tcacggcgac gttttccagt
     9781 aatgacggtg tgtgttgctg agtggcaggc gaaagcggac gaatcagtgc cgccgccagc
     9841 aaggtggctt ccggcagcga actcactacc gcgatccctg catcttccag cgtggcgatt
     9901 tgctgcgagc ggcattgcgg gtcacgttca gtgcctgtca ccgtggcaat ggcatacagt
     9961 ggttgattgt ctgaacgcgc ggcacatgct ttttgccagg cgcttaccag cgaggcggca
    10021 ggatcggcgg tcgcaccgaa gccaatcacg acatcaagca gcaatacgcg cacttgcggt
    10081 ttagcgccga gatcggcaat taactggttg cgtaaggctg ggtcgatcat cggatgggga
    10141 cgcccgacgg tgtagaaatc atcgccgagg tcaataatct ggtgaccatc ggcgtcgagc
    10201 atcatgccgt gatgatgggt atcgtcggct tccacgccaa ggtgtccagc aagtaatccc
    10261 gccgcttcgg cagccagcgt accgccggta tacaaaccgc aaataaatcc gctgctgaca
    10321 ggcgctattg cgttacgtcg cgccgtgaca cgtgaaagca ggcaagccag gcgtgcggcc
    10381 tcatccagcg aggaggcaaa ccagacattc tcgtcgcggg ccaccgccgg ggtataacct
    10441 aaaaacagcg ccaccgtcgg tttgccggtt gctttcatgg cattaacaat tttcagacgc
    10501 acagcttcgg caggcggttt tgaaacaaat gccagcactt cgcttttctc gtctgcactg
    10561 agcatttcca gcgccgtcag cgcactgatg ccgcctactt cacggctgag gtcgcgcccg
    10621 ccaaggccaa tcgcgtgagt aattccctcc cctgccagcg caatctgcga gcacagctcc
    10681 tgaatcccgg taccggaagc gccaatgacg ccaatgttgc cttccggcat cacgttagca
    10741 aaagccagcg gcgtgccggc aatcatcgat gtaccgcagt ccggccccat caccagcaag
    10801 cctttttccc gcgcgcgggt tttaagttgg atttcatctt ccagcgtgac gttatcggag
    10861 aacatcatca cgttgaggtt gcgatccagc gcctggttcg ccagctccgc cgcatactca
    10921 ccagccactg aaatcagcgc cagactggcg tcgggtaatt tctgacaggc actgtcccag
    10981 cgacgcacct gcgtcaacgc ctggctgctg cctgccccct gcgccagttg ttttagcgcc
    11041 tcttcaagct gctgcataat cgcctgcgcg atccccgcat ccgccgcttc gctacgaatt
    11101 gccacgcaaa tatcgttcgg cgtggcgtta ttaaaatcgt catgccagaa acctgtggta
    11161 tctaataacg ctttattggc gggcgtaccc atcattacgg aaacatcatc aacattttct
    11221 gattcgctga gttttcgtga aataatcatt aaactgaccg aatcctgaaa acaccctttt
    11281 ttaataaagg cgtggatcat aacaactcct taaaaattgc acccatggca attgcacgag
    11341 cagtgttaat gtggcgatta cggtatcgat gacaacgggt aaataaggtg atgaatatca
    11401 cacagggatt attaacaaaa gaattca
//
LOCUS       DBANUU010000135        11374 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000135 DBANUU010000000
VERSION     DBANUU010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.136"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            31..2421
                     /gene="gcd"
                     /locus_tag="ACIE68_003764"
     CDS             31..2421
                     /gene="gcd"
                     /locus_tag="ACIE68_003764"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFX5910016.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWLSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKNPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            2460..2558
                     /locus_tag="ACIE68_003765"
                     /pseudo
     CDS             2460..2558
                     /locus_tag="ACIE68_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2623..4173)
                     /gene="cueO"
                     /locus_tag="ACIE68_003766"
     CDS             complement(2623..4173)
                     /gene="cueO"
                     /locus_tag="ACIE68_003766"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFX5910017.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQHGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            4339..4686
                     /gene="yacC"
                     /locus_tag="ACIE68_003767"
     CDS             4339..4686
                     /gene="yacC"
                     /locus_tag="ACIE68_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFX5910018.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            4792..5658
                     /gene="speE"
                     /locus_tag="ACIE68_003768"
     CDS             4792..5658
                     /gene="speE"
                     /locus_tag="ACIE68_003768"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFX5910019.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLTHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVKQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            5674..6468
                     /gene="speD"
                     /locus_tag="ACIE68_003769"
     CDS             5674..6468
                     /gene="speD"
                     /locus_tag="ACIE68_003769"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFX5910020.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(6506..6868)
                     /gene="yacL"
                     /locus_tag="ACIE68_003770"
     CDS             complement(6506..6868)
                     /gene="yacL"
                     /locus_tag="ACIE68_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFX5910021.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(7043..9640)
                     /gene="acnB"
                     /locus_tag="ACIE68_003771"
     CDS             complement(7043..9640)
                     /gene="acnB"
                     /locus_tag="ACIE68_003771"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFX5910022.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            9995..>11374
                     /locus_tag="ACIE68_003772"
     CDS             9995..>11374
                     /locus_tag="ACIE68_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFX5910023.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHRDGNGWQHNGDNINIDVNNFNR
                     ITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSATQLPAPTRDSQRQ
                     AAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFSRRQPLTQQQKDAA
                     RQRYQSASPE"
BASE COUNT         2690 a         3154 c         2861 g         2669 t
ORIGIN      
        1 aatattaatg aattgaaatg gtgtctcttt atggcaatta acaatacagg ctcgcgacga
       61 ttactcgtca cgctaacagc cctttttgca gcgctttgcg gactgtatct actcattggc
      121 ggaggctggc tggtcgcgat tggcggctcc tggtactacc ctatcgctgg tcttgtgatg
      181 ctcggcgtcg cctggatgct gtggctcagt aaacgtgccg cgctttggct gtacgcagcc
      241 ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg gtttcgactt ctgggcgctg
      301 actccgcgca gcgacattct ggtcttcttc ggcatctggc tgatcctgcc gtttgtctgg
      361 cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac tggtggtggc actgctgatt
      421 agcggtggta tcctgacctg ggccggattt aacgatccgc aggaaatcaa cggcacctta
      481 agcgccgatg ccacacctgc cgaagcaatc tcccccgttg ccgatcagga ctggcctgcc
      541 tatggtcgta atcaggaagg tcaacgcttt tcacctctga aacaaattaa cgccgataac
      601 gttcacaagc tgaaagaagc ctgggtgttc cgtactggcg atgtgaagca gccgaacgat
      661 ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg gcgacaccct ttacctgtgt
      721 accgctcacc agcgactgtt tgcgctcgat gccgccagcg gcaaagagaa atggcattac
      781 gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa cctgccgtgg cgtctcttat
      841 catgaagcca aagcagaaac ggcttcgccg gaagtgatgg cggattgccc gcgtcgtatc
      901 attcttccag tgaatgatgg tcgcctgatt gcgattaacg ctgaaaacgg caaactgtgc
      961 gaaaccttcg ccaataaagg cgtgcttaat ctgcaaagca atatgccaga caccaaaccg
     1021 ggtctgtatg aaccgacttc gccaccgatt atcaccgata aaaccatcgt gatggcaggt
     1081 tcagttaccg ataacttctc aacccgcgaa acgtctggcg tgatccgtgg ttttgatgtc
     1141 aacaccgggg agctgctgtg ggcttttgat ccgggcgcga aaaatccgaa cgcaatcccg
     1201 tctgacgaac acacctttac ctttaactcg ccaaactcgt gggcaccagc ggcctatgac
     1261 gcgaagctgg atctggtgta tctgccgatg ggcgtgacca cgccggatat ctggggcggt
     1321 aaccgcacac cggaacagga acgttatgcc agctcgattc tggcgctgaa tgccactacc
     1381 gggaaactgg cgtggagcta ccagaccgtt caccacgacc tgtgggatat ggatcttccg
     1441 gcacagccga cgctggcgga catcaccgtt aatggtcaga aagtgccagt tatttacgct
     1501 ccggcgaaaa ccggcaacat ttttgtgctc gatcgtcgta atggcgaact ggtggttccg
     1561 gcaccggaaa aaccggttcc ccaaggtgct gccaaaggcg attacgttac ccctactcaa
     1621 ccattctcgg aactgagctt ccgtccgacg aaagatttga gcggtgcgga tatgtgggga
     1681 gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc accagatgcg ctatgaaggc
     1741 attttcaccc cgccatctga acagggtacg ctggtcttcc cgggtaacct ggggatgttc
     1801 gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg cgattgccaa cccaatggca
     1861 ctgccgtttg tttcgaaact gatcccgcgt ggtcctggta acccgatgga gcagccgaaa
     1921 gatgccaaag gcacgggtac ggaatccggc attcagccac agtacggtgt accgtatggt
     1981 gtcacgctca acccgttcct ctcaccgttt ggtctgccat gtaaacagcc agcatggggt
     2041 tatatctcgg cgctggatct gaaaaccaat gaagtggtgt ggaagaaacg tattggtacg
     2101 ccgcaggaca gtatgccgtt cccgatgcct gtaccggtgc cgttcaatat gggtatgccg
     2161 atgctgggcg ggccaatctc cacggcgggt aacgtgctgt ttatcgccgc tacggcagat
     2221 aactacctgc gcgcttacaa catgagcaac ggtgaaaaac tgtggcaagg tcgtttacca
     2281 gcgggtggtc aggcgacgcc aatgacctat gaagtgaatg gtaagcagta tgtggtgatc
     2341 tccgcaggcg gtcacggttc atttggtacg aagatgggcg actatattgt ggcttatgcc
     2401 ctgccggatg atgtgaagta agacttgctc agattgctga caacgtgcgc gttgttcatg
     2461 ccggatgcgg cgttaacgcc ttatccagcc tacaaaattg tgcaaaatca atggattgca
     2521 caggtagcgt aggcctgata agcgtagcgc atcaggcaga tttgcgtttg tcatcagttt
     2581 aatgcccgga gagatccggg catatttccg aatacggtct ttttataccg taaaccctaa
     2641 catcatcccc gtatcttcat gctccagcag atggcagtgc gccatataag catgttcttt
     2701 cggtgcatcg tgattaaact tcaccagcac ttcgctgaca ttaccttcta ccttaacggt
     2761 atctttccag cccgcgcgat gagccgctgg cggtttgcca ttttctgaca agatacggaa
     2821 ctgcgtgccg tgaatatgga acggatgcag catcatgtca cccacgccag agataaccca
     2881 acgttcgtac tgccctttcg ccgccgcaaa catcggctta ttcatatcaa acgcctgacc
     2941 gttgatttta ttggcatggt ggaaatcgaa cttcccgccg tggttcatat gattcatatt
     3001 gccgtgcccc atatggccca tcatctggct gtgatccatc ccggccatcg cctgatcgcc
     3061 atatttctcc atcagcatct gcatacccat catatcgagc atcgggtcca tagaaagttg
     3121 cagcttgcgt accgtcagcc cttccagcga aggtaacgca ggcaggctac ttaatgtgtc
     3181 tggcaaagca ccggaggcac taatagcaat cggctgaatc cgcattaccg gatgcggctt
     3241 gtcaaatggc gcaatcgcca tccccatctg gctgaccggc agcgtcacca ggtcaaaggg
     3301 tttgttgtcg ttaacctcca ccagcacttc aaaacgctcg cccatcagca ccggcagctc
     3361 gttcaccttc accggttcag gtagcagacc accgtcgctg gcaatcacat aaagcgggcg
     3421 attgtcgctg gtggcgaaat tgagcgagcg ggcattacag ccattgagca aacgcaggcg
     3481 cagccaacca cgcggggcag cgtgttgcgg gtagattgca ccgttggtca gcaacgtatc
     3541 gccaaaccag cccacggcgg cggtcatcac atccagttga taatcaatct gcccgtcggc
     3601 gctaaatttc ttatcctgaa cgatcaccgg aacatcatcg ataccccact gttttggcag
     3661 cattaatttc aggatctcgt catcttcaat taccaccaac ccagccaacc ccatcgccac
     3721 ctgtcgcccg gtcttgccat gttgatgtgg atggaaccag caggtagcgg caggttgatc
     3781 aacgttcaac gtcaccgagc gcttgccacc tggcggaata attccctgcg ggccgccgtc
     3841 cacttcaccc ggtacttcca gcccgtgcca gtgcaacgtc gtctcttccg tcagttggtt
     3901 atagatatca accgttaccg ctttgccgtg ctgtaatttc accgccggcc ccagcagatt
     3961 gccgttatag ccccaggtag ttgcagtttt cccgccaaag gtggactggc ctgcgccaat
     4021 agttaactga atgcgattac gggcatcggt cgtgagcaaa tcagggatcg gtaacgttgg
     4081 gcgttccgcc gcaaagactg cgcggctcca cagcggcaag gctgaagcca cacccagcgc
     4141 gacggaatat tttaagaaat cacggcgttg catagttatt tccttattct taagcaggcg
     4201 aaacaaaatc aaacgttgag catagtcctt ccccttacgg gaaggtcaag ccgcagacac
     4261 aatttaatcg acaataatgg tcgtcgcctc gctcgcagtg tgctaacgtt tatcttcttt
     4321 aagcccctgg tagaagcaat gaagacgttt ttcagaacag tgttattcgg cagcctgatg
     4381 gccgtctgcg caaacagtta cgcgctcagc gagtctgaag ccgaagatat ggccgattta
     4441 acggcagttt ttgtctttct gaagaacgat tgtggttacc agaacttacc taacgggcaa
     4501 attcgtcgcg cactggtctt tttcgcccag caaaaccagt gggatctcag taattacgac
     4561 accttcgaca tgaaagccct cggtgaagac agctaccgcg atctcagcgg cattggcatt
     4621 cccgtcgcta aaaagtgcaa agccctggct cgcgattcct taagcctgct tgcctacgtc
     4681 aaataatccc tgataccttt ttgtagaaat attgaccgtg catccgcggt caatgttagc
     4741 tattatgttg cgcccttttt ttacgggtgt taacaaagga ggtatcaacc catggccgaa
     4801 aaaaaacagt ggcatgaaac gctacacgac cagtttgggc agtactttgc ggtagataac
     4861 gttctgtatc atgaaaagac cgatcaccag gatctgatca tttttgagaa cgctgcattt
     4921 ggtcgcgtaa tggcgctgga tggcgtagta caaaccaccg agcgcgacga gtttatctat
     4981 catgagatga tgactcatgt tccgctactg acccacggtc acgcgaaaca tgtgctgatt
     5041 atcggcggcg gcgacggtgc catgctgcgt gaagtaaccc gacataaaaa cgttgagtca
     5101 atcacgatgg tggaaatcga tgcgggtgtc gtatcgttct gccgtcagta tctacccaac
     5161 cataacgccg gtagctacga cgatccgcgc tttaagctgg tgatcgatga tggcgtcaat
     5221 ttcgttaaac aaaccagcca gacctttgat gtcattatct ccgactgcac cgatcctatc
     5281 ggtcccggcg aaagtctgtt cacctcagca ttttatgaag gctgcaaacg ttgcctgaat
     5341 cctggcggta tcttcgtcgc acaaaacggc gtctgctttt tacagcagga agaagccatc
     5401 gacagccatc gcaaactcag ccattacttc agcgacgttg gcttttatca ggcggcgatc
     5461 ccgacctatt acggcggtat catgactttt gcatgggcga cagataacga cgccttacgc
     5521 catctctcaa ccgaaattat tcaggcgcgt tttctcgctt ctggcctgaa atgccgttat
     5581 tacaatccgg caatccatac ggcagctttt gccttacctc agtatctgca agacgcactg
     5641 gcttcacagc cgtcctaagg agaagataag aaattgaaaa aactgaaact gcatggcttt
     5701 aataatctga ccaaaagtct gagtttttgt atttacgata tctgctacgc caaaactgcc
     5761 gaagagcgcg acggttatat tgcttatatc gatgaactct ataatgccaa ccgtctgacc
     5821 gaaatcctgt cagaaacctg ttccattatc ggggccaata ttcttaacat cgcccgtcag
     5881 gattacgaac cacagggtgc cagcgtcact attctggtga gtgaagaacc ggtcgacccg
     5941 aaacttatcg acaaaacaga acaccccggc ccactgccag aaacggtggt tgcccatctc
     6001 gataaaagtc atatttgcgt acatacctac ccggaaagtc atcctgaagg cggtttatgt
     6061 accttccgcg ccgatattga agtctctacc tgcggcgtga tttctccgct gaaggcgctg
     6121 aattacctga tccaccagct tgagtccgat atcgtaacca ttgattatcg cgtgcgcggt
     6181 tttacccgcg acattaacgg tatgaagcac tttatcgacc atgagattaa ttcgattcag
     6241 aactttatgt ctgacgatat gaaggcgctg tatgacatgg tggatgtgaa cgtctatcag
     6301 gaaaatatct tccataccaa gatgttgctt aaagagttcg accttaagca ctacatgttc
     6361 cacaccaaac cggaagattt aaccgacagc gagcgccagg aaattaccgc tgcgctgtgg
     6421 aaagaaatgc gcgagattta ttacgggcgc aatatgccag ctgtttaacg gctctggcgg
     6481 agctcccagg ctccgccaga tttatttact tctgctgcac gaaattgcgg taagccgcca
     6541 cgacctgcag aaaatcctca acgccgcata gcgacaggct ttcttcgtcg tagtagttca
     6601 tcccttcttc catttcatcg ccagcgaatt ccagttgatt ggcgcgaacc atcacctctt
     6661 caccgtccat ccacagggtg tattcatgcc ctgcccgttg ccaggaccgt tcgctacctt
     6721 tcagtgcgtg cgctgcttgt tccacttcat caagcaaagc caggttttct ttcacctctt
     6781 cattaaacca gtgcccgacc acttcatgcc ccatggacat acgcaccttt accactccgg
     6841 taatatcgcg cagaaattcg taatccatag tgtgttcctc tgctcccggc aatgcgccga
     6901 aatcgcattg cgccattgat gtaattatcg caaccttacc ggagaaaaac agcggaacgg
     6961 cgatggttta gaaataaaaa atgcccggga atctcattcc ccgagcattg tgtcgtttat
     7021 gcgcagcgcg tgcgctgact ttttaaaccg cagtctggaa aatcacccca tcggcttttt
     7081 cggtatactg agaaagctgg ttgaagttca gataacgata agtatcaacg gcagttttat
     7141 ccacttgcgc cacgtaggtc tggtactctt ccggcgtcgg cagtttgcca atcagcgccg
     7201 caacagccgc cagttccgca gaagccagga agacattcgc gccagtaccc agacggttcg
     7261 ggaagttacg ggtagaggtg gaaaccaccg tcgcaccgtc tgctacacgc gcctggttac
     7321 ccatacacag ggaacagcca gggatctcga tacgcgcacc actcttaccg aagacgctgt
     7381 agtagccttc ttcagtcaac tgcgcggcgt ccatacgggt tggcggtgcc acccacaggc
     7441 gggtcggcag ttggcctttg tgcgcatcca gcagtttacc cgcagcacgg aagtgaccga
     7501 tgttggtcat gcaggaaccg ataaatactt cgtcgatctt ctcaccctgt accgcagaca
     7561 gcggacgcgc gtcatccggg tcgttcggtg cacagaggat tggctcttta atatccgcca
     7621 gatcgatgtc gatcactgcc gcgtattcag catctgcatc ggcttccagc agctcaggat
     7681 tcgccagcca tttttccatg ccctgaatac gacgttccag ggtgcgacga tcgccgtaac
     7741 cttccgcgat catccacttc agcaggacga tgttagagtt caggtattcg atgatcggtt
     7801 ctttgttcag cttgatggta caaccagcgg cagaacgctc agcggacgca tcggtaagct
     7861 caaaggcctg ctcaactttc agatccggca gaccttcaat ttccagaatg cggccagaga
     7921 agatgttttt cttgcctttc ttctcaacgg tcagcagacc ttgtttgatg gcatacagcg
     7981 gaatggcgtg taccagatcg cgcagggtga tgcccggctg cattttgcct ttgaagcgca
     8041 ccagaacgga ttccggcata tccagcggca ttacgcctgt tgcggctgca aatgccacca
     8101 gaccagaacc cgccgggaaa gagataccga tcgggaaacg ggtatgggag tcacctccgg
     8161 taccgacggt atccggcagc agcatacggt tcagccagga gtggattacg ccgtcacccg
     8221 gacgcagcga cacaccgcca cggttcataa tgaagtccgg cagcgtgtgg tgcgtgttca
     8281 cgtcaactgg cttcggatac gccgcggtgt ggcagaaaga ctgcatcacc aggtcagccg
     8341 agaagcccag gcacgccagg tctttcagtt catcacgggt catcgggccg gtggtgtcct
     8401 gagagcctac agaagtcatt ttcggttcgc agtacgcgcc cggacgaatg cctttcacgc
     8461 cacaggcacg gcctaccatt ttctgcgcca gcgagaagcc gcgatcgctc tcagcgacat
     8521 ctttcgcctg acggaacaca tcactgtgcg gcagaccaag tgcttcacgc gctttggtgg
     8581 tcaggccacg cccgataatc agcggaatac ggccaccagc acgcacttca tcaatcagca
     8641 cgtcggtttt cagttcgaag gtcgccagca gttcgccggt ttcgtggtta cgcacttcac
     8701 ctttgtacgg gtaaacgtca atcacgtcgc ccatgttcag gttagagacg tcgacttcga
     8761 ttggcagtgc acccgcgtct tccatcgtgt taaagaagat gggtgcaatt ttaccgccga
     8821 ggcacaaacc accgccgcgt ttgttcggca catgtggaat atcatcgccc ataaaccaca
     8881 gaacggagtt agtggcggat ttacgcgaag aacccgtacc cacaacgtca ccgacgtacg
     8941 ccagcgggaa acctttctgt tgcagagctt cgatttgctt gatcggacca acaacaccag
     9001 gctggtctgg ctcaatacct tcacgggcgt ttttcagcat cgccagcgcg tgcagtggga
     9061 tatccgggcg tgaccacgca tccggtgccg gagaaaggtc atcggtgtta gtttcgccag
     9121 tgactttgaa gacagtaacg gtcagttttt cagccagcgc cgggcgattc aggaaccatt
     9181 cggcatccgc ccaggactgc ataacctgct tcgcatattc gttgcctgct ttcgctttct
     9241 cttctacgtc atagaagtta tcgaacatca gcagcgtgtg agaaagtgct ttggcagcaa
     9301 taggtgccag tttggcatca tccagcgcgt cgatcagcgg atgaatgttg taaccaccct
     9361 gcatggtgcc cagcagttcg atggcttttt ccggagtcag cagaggggat ttggcttcgc
     9421 ctttcgcgat agcagccagg aagcctgctt tgacataggc ggcttcatcg acgcctgggg
     9481 gaacacggtt ggttaacaga tctaacagga attcttcttc gcccgcgggc gggtttttca
     9541 gcagctctac aagtgcggcc atttggtttg catccagggg tttgggcgca atcccctcag
     9601 cggcacgctc agctacgtgc ttacggtatt cttctagcac gacggttctc ctcgctctca
     9661 ttgtcatagt gcggcaggcg attctcttca cgctcctgtg agacagcagt ttgtagggta
     9721 aatgcccagt accgcaacgg gcagaatagc aggattttga cgatgtgtta atctgtttac
     9781 aaaaaagcaa cataaaaaag tggctgaatc gttaaggatg gtctagagat tgtttccctc
     9841 cgtaaaaact tcgttaattc gcatggtgat aatcaccttt ctcaacgaat cccaagatta
     9901 gtcaaaattt aaactaccgc ctctttatac tcggattcac agcacctgcg ggtggcagtt
     9961 cgccgaccat tgcgatttcc ttgagatccg aattatgaaa atgactttgc cgtttaaacc
    10021 ccatgtgctg gcgctaattt gcagtgccgg gctttgtgcc gcctctaccg ggctatatat
    10081 aaaaagccgc acagtggaag cgcctgtgga accgcaatcg acacaacaga ctgcgcctga
    10141 catcaccgca gttacgcttc ctgcaacggt ttccgcaccg cccgtaacgc ccgccgtcgt
    10201 caaatccgca ttcagcactg cacaaatcga tcaatgggtt gcgcctgtcg cgctgtaccc
    10261 cgattctctg ctttcacaag tgttaatggc atcaacctat ccggcaaacg ttgctcaagc
    10321 agtgcaatgg tcgcacgata atccacttaa acaaggcgat gctgctattc aggcggtatc
    10381 tgaccagccg tgggacgcca gcgttaaatc actggtggcc tttccacaat tgatggcatt
    10441 gatgggcgaa aacccgcaat gggtgcaaaa cctgggcgat gcttttctgg cgcagccgca
    10501 ggacgtgatg gactcggtac agcgattgcg gcaactggcg caacaaaccg gttcgctgaa
    10561 gtcatcaacc gaacaaaaag ttattaccac aacgaagaaa gctgtaccgg taaaacagac
    10621 agtcacggca cccgtcatac catccaatac cgttttaact gccagccccg tcattacaga
    10681 gcctgcaaca accgtcattt ccattgagcc cgccaatcct gatgtggtct atattcccaa
    10741 ctacaaccca accgtggttt acgggaactg ggccaatact gcgtatccgc cggtttatct
    10801 gccaccacca gccggagaac cgtttattga cagctttgtg cgcggattcg gctatagcat
    10861 gggtgttgct accacgtacg cactattcag cagcatcgac tgggatgacg acgatcatga
    10921 ccatcatcat cgtgacggta atggctggca acacaacggc gacaacatca atatcgacgt
    10981 caacaatttc aaccgtatca ccggtgagca tcttactgat aagaatatgg catggcggca
    11041 caatccaaac taccgtaatg gtgtgcccta tcatgatcag gatatggcaa agcggtttca
    11101 tcaaaccgat gtcaacggcg gaatgagcgc cacgcagcta cctgctccaa cacgcgacag
    11161 ccagcgtcag gcggcagcaa gtcagtttca gcaacgaaca cacgccgccc ccgtcattac
    11221 acgagatacc caacgtcagg cagcggcaca gcggtttaat gaagctgaaa actatgggag
    11281 ctatgacgac ttccgcgact tcagccgtcg ccaaccactg acccagcaac aaaaggacgc
    11341 cgctcgtcag cgttatcagt cggcctcgcc tgag
//
LOCUS       DBANUU010000136        11358 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000136 DBANUU010000000
VERSION     DBANUU010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.137"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(99..230)
                     /locus_tag="ACIE68_003773"
     ncRNA           complement(99..230)
                     /ncRNA_class="other"
                     /locus_tag="ACIE68_003773"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(277..408)
                     /locus_tag="ACIE68_003774"
     ncRNA           complement(277..408)
                     /ncRNA_class="other"
                     /locus_tag="ACIE68_003774"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(455..539)
                     /locus_tag="ACIE68_003775"
     tRNA            complement(455..539)
                     /locus_tag="ACIE68_003775"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(503..505),aa:Tyr,seq:gta)
     gene            complement(699..1541)
                     /gene="purU"
                     /locus_tag="ACIE68_003776"
     CDS             complement(699..1541)
                     /gene="purU"
                     /locus_tag="ACIE68_003776"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFX5910024.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(1591..2049)
                     /gene="ychJ"
                     /locus_tag="ACIE68_003777"
     CDS             complement(1591..2049)
                     /gene="ychJ"
                     /locus_tag="ACIE68_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFX5910025.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            2162..3067
                     /gene="rssA"
                     /locus_tag="ACIE68_003778"
     CDS             2162..3067
                     /gene="rssA"
                     /locus_tag="ACIE68_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFX5910026.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            3159..4172
                     /gene="rssB"
                     /locus_tag="ACIE68_003779"
     CDS             3159..4172
                     /gene="rssB"
                     /locus_tag="ACIE68_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFX5910027.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            4374..5282
                     /gene="galU"
                     /locus_tag="ACIE68_003780"
     CDS             4374..5282
                     /gene="galU"
                     /locus_tag="ACIE68_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFX5910028.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(5426..5839)
                     /gene="hns"
                     /locus_tag="ACIE68_003781"
     CDS             complement(5426..5839)
                     /gene="hns"
                     /locus_tag="ACIE68_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFX5910029.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            6443..7060
                     /gene="tdk"
                     /locus_tag="ACIE68_003782"
     CDS             6443..7060
                     /gene="tdk"
                     /locus_tag="ACIE68_003782"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFX5910030.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFAEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            complement(7362..10037)
                     /gene="adhE"
                     /locus_tag="ACIE68_003783"
                     /gene_synonym="adhC"
     CDS             complement(7362..10037)
                     /gene="adhE"
                     /locus_tag="ACIE68_003783"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFX5910031.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            10430..10483
                     /gene="ychE"
                     /locus_tag="ACIE68_003784"
     CDS             10430..10483
                     /gene="ychE"
                     /locus_tag="ACIE68_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFX5910032.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            10514..11161
                     /gene="ychE"
                     /locus_tag="ACIE68_003785"
     CDS             10514..11161
                     /gene="ychE"
                     /locus_tag="ACIE68_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFX5910033.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
BASE COUNT         2884 a         2559 c         2815 g         3100 t
ORIGIN      
        1 atccgaccta caggggagga tattgtaggt ccggtaaacg tggtgccatc gggcaaaatt
       61 agctcggagt aacaggtttt gattatttca ggaagcgatg gtggtggggg aaggattact
      121 cagcgctgcg cgcttcgccc ttcgggtcgt tgcctgcggc aacgctctct cgctggcgct
      181 cgagtcgaac cttagtcgaa gcttctcatc cttccccgca tgggcagaat atttaattgc
      241 ggattcgctt gaaagttcag ggacttttga aagtgatggt ggtgggggaa ggattactca
      301 gcgctgcgcg cttcgccctt cgggtcgttg cctgcggcaa cgctctctcg ctggcgctcg
      361 agtcgaacct tggtcgaagc ttctcatcct tccccgcatg ggcagaatat ttaattacgg
      421 attcgtttga gagttcaggg acttttgaaa gtgatggtgg tgggggaagg attcgaacct
      481 tcgaagtcga tgacggcaga tttacagtct gctccctttg gccgctcggg aaccccacca
      541 cggggtaatg ctttttactg gcctgctccc ttatcgggaa gcggggcgca tcatatcaaa
      601 tgacgcgccg ctgtaaagtg ttacgttgag aaaaatgaac tggttgcgta attttcatcc
      661 gtaacggatt aaaggtaacc agttattttt gctggcgatt aaagaataat cgtccgatta
      721 ccgtaaacaa agacgcgctg tgccagtact ttgtatagcg cacgacttaa gacgtttttc
      781 tcgacgtcac gacctgcgcg catcatatct tcagctgtgt aggtatgatc gacatgaata
      841 acgtcctgca tgatgattgg gccttcgtcc agattgtcat tcacatagtg agcggttgcg
      901 ccaataatct tcacaccacg ttcataggcc tggtgataag gacgtgcgcc gataaacgca
      961 ggcaggaatg aatggtgaat attgatgatt ttattcggga agcgtgccac aaattccggc
     1021 gttaataccc gcatatactt cgccagcacc acatagtcag gttgataagc atcaatggcg
     1081 tccgccatct tttgatcgtg ctcgttgcgg cttaaccctt catggcttac cagctcaaac
     1141 ggaatatcaa aacgctcaac cagagaacgt aaagtatcgt ggttaccaat aactgccgcg
     1201 atttcgacat ccaggccgcc gtaattggct ttcatcaaca aatcgccaag gcaatgcgct
     1261 tctttagtga ccagaatcac tatccggcga cgaccggcag gattcagctc acgcacggag
     1321 ccttctggca atgcgctatc gagatccgcc agaagggtgg aatcattaaa aatcccttcc
     1381 agttccgtgc gcataaaaaa gcgcccggta cggtgatcaa caaattcatt gttctgtacg
     1441 atatttaact cgtgcttgta gcaaatattg gtaatacgtg cgatcagacc tttttggtcc
     1501 ggacaaatag tacgcagaac tttacgttgg agtgaatgca ttgctggaaa aaccttgttg
     1561 agagtgtttg ctaaaccgta ccgtcaacca ttattggccg cagcactttt taaatttttt
     1621 acctgaacca caagggcagg gatcgttgcg accaaactgc ggacgtgtac cgtcaatata
     1681 gtaccattga ccgttttctt tcaaaaatcg cgaacgttca attattgcac ctgttttgcc
     1741 accttcggta aatcgagcaa caaaactgac aaatccgata ttgtcggcgt cctgccaaca
     1801 atgttcaaat acggtcaatc ccaaccattc agtatgcgca aatccggcca tcaattcggc
     1861 acgtaacgcc gctgctccac aagagggatg ccaggtcttt attaaataat ctgcgtcttg
     1921 catcacaaaa gcgcagtaac gcgaacgcat gagatgttct ggatcaggtg caaccttttc
     1981 accagacaca taagggtggc aacataggct atactcgaca gcactaccac agggacaaag
     2041 ctgagacaca aataatctcc ctggaaacaa taacggcgta ttaaccgcct gagtagcact
     2101 atgttaaccg agcagtagcg atgtggctac gattgcattc caggggaatc ttgcgggaat
     2161 aatgagaaag ataaaaatag ggctggcgct gggatctggc gcggcgagag gttggtcgca
     2221 tattggcgtt attaatgcgc taaaaaaagt gggtattgaa attgatatcg ttgcaggatg
     2281 ttcaattggt tcgctggtgg gcgcggccta tgcatgcgat cgattatctg cgctggaaga
     2341 ttgggtaacc tctttcagtt attgggacgt tttacgcctg atggatctct cctggcagcg
     2401 cggtgggtta ctgcgcggcg agcgtgtctt caatcaatac cgcgaaataa tgccggaaac
     2461 agagatcgaa aattgttccc gtcgctttgc ggctgttgcc accaatttaa gtacgggacg
     2521 tgaattatgg tttactgaag gcgatctcca tcttgctatt cgcgcatcat gcagtattcc
     2581 aggactcatg gcacctgttg cacataacgg ctactggctg gttgatggag cagtcgttaa
     2641 cccaattcct atttcactca cgcgtgcatt gggtgctgat attgtgatag cggtcgacct
     2701 gcagcacgat gctcatttga tgcaacaaga tttgctctcc tttaatgtca gtgaagaaaa
     2761 tagcgagaat ggtgattctc tgccgtggca tgcgcgtctg aaagaaaggt taggcagcat
     2821 aacgacacgt cgggcggtga cagcaccaac ggcaacagag attatgacca cttctatcca
     2881 ggtgctggag aaccgcctta aaaggaaccg catggcaggt gatccgcccg atattctgat
     2941 tcaacctgtt tgcccgcaaa tatctacgct tgatttccat cgcgcgcacg ctgccattgc
     3001 ggccggacag ctggcagtgg aaaagaaaat ggacgaactt ttgccgttgg tacgcaccaa
     3061 catttgacca gaatttttat ctacacttaa gttaattctg acaggcgcag gtggcaatag
     3121 catgccacta ttgagtaaag ccagtcaggg gagagaacat gacgcagcca ttggtcggaa
     3181 aacagattct cattgttgaa gatgagcagg tatttcgctc gcttctggat tcgtggtttt
     3241 cctcattggg agcgacaacg gtactggcgg ctgatggggt ggatgccctt gagttgctgg
     3301 gaggtttcac tccagacctg atgatatgtg atatcgcgat gccacgaatg aacgggctta
     3361 aactgctgga gcatatacgt aacagaggcg accagacccc agttctggtg atatctgcca
     3421 ctgaaaatat ggcagatatt gccaaagcgt tacgtctggg cgttgaagat gttttgctga
     3481 aaccagttaa agatctgaat cgcttgcgcg agatggtttt tgcctgtctc tatcccagca
     3541 tgtttaattc gcgcgttgag gaagaggaaa ggctttttcg cgactgggat gcaatggttg
     3601 ataaccctgc cgcagcggcg aaattattac aggaactaca accgccggtt cagcaggtga
     3661 tttcccattg ccgggttaat tatcgtcaat tggttgccgc ggacaaaccc ggcctggtgc
     3721 ttgatattgc cgcactttcg gaaaacgatc tggcatttta ttgccttgat gtaacccgag
     3781 ctggacataa tggcgtactt gctgccttgt tattacgcgc attgtttaac ggattattac
     3841 aggaacagct tgcacaccaa aatcaacggt tgccagagtt gggcgcgtta ttgaagcagg
     3901 taaaccattt actccgtcag gccaatctgc cggggcagtt tccgctatta gttggctatt
     3961 atcatcgcga actgaaaaat ctcattctgg tttctgcggg tctgaatgcg acgttaaata
     4021 ccggcgaaca ccaggtgcaa atcagtaatg gtgttccgtt aggcacttta ggtaacgctt
     4081 atttgaatca attgagccag cgatgcgatg cctggcaatg ccaaatatgg ggaaccggtg
     4141 gtcgactgcg cttgatgttg tctgcagaat gagcaaacga taacgcgggc taaatttgca
     4201 ttacctgcta atgtcggctg gtggtactat cgtcgccatt cgtataagta attgtcttaa
     4261 ttatgctaac tcgcctcctt ttcagaactt agccccttcg gggtgctgat atactgggat
     4321 gcgatacaga aatatgaaca cgttcaaaac acgaacagtc caggagaatt taaatggctg
     4381 ccattaatac gaaagtcaaa aaagccgtta tccccgttgc gggattagga accaggatgt
     4441 tgccggcgac gaaagccatc ccgaaagaga tgctgccact tgtcgataag ccattaattc
     4501 aatacgtcgt gaatgaatgt attgcggctg gcattactga aattgtgctg gttacacact
     4561 catctaaaaa ctctattgaa aaccactttg ataccagttt tgaactggaa gcaatgctgg
     4621 aaaaacgtgt aaaacgtcaa ctgcttgatg aagtgcagtc tatttgtccg ccgcacgtga
     4681 ctattatgca agttcgtcag gggctggcga aaggcctggg acacgcggta ttgtgtgctc
     4741 acccggtagt gggtgatgaa cctgtggctg ttattttgcc tgacgttatt ctggatgaat
     4801 atgaatccga tttgtcacag gataacctgg cagagatgat ccgccgcttt gatgaaacgg
     4861 gtcatagcca gatcatggtt gaaccggttg ctgatgtgac cgcatatggc gttgtggatt
     4921 gcaaaggcgt tgaattagcg ccgggtgaaa gcgtaccgat ggttggcgtg gttgaaaaac
     4981 caaaagcgga tgttgcgccg tctaatctcg ctattgtggg tcgttacgta cttagcgcgg
     5041 atatttggcc gttgctggca aaaacccctc ccggagctgg tgatgaaatt cagctcaccg
     5101 acgcaattga tatgctgatc gaaaaagaaa cggtggaagc ctatcatatg aaagggaaga
     5161 gccatgactg cggtaataaa ttaggttaca tgcaggcctt cgttgaatac ggtattcgtc
     5221 ataacaccct tggcacggaa tttaaagcct ggcttgaaga agagatgggc attaagaagt
     5281 aacatccgta tcggtgttat ccacgaaacg gcgttgagca atcgacgccg tttttttata
     5341 gcttattctt attaaattgt cttaaatcgg acaataaaaa atcccgccga tggcgggatt
     5401 ttaagcaagt acaatctaca aaagattatt gcttgatcag gaaatcgtcg agggatttac
     5461 cttgctcatc cattgctttt ttgattacag ctggagtacg gccctggcca gtccaggttt
     5521 tagtttcgcc gttttcgtca acgtagctat attttgccgg acgctgagca cgtttagctt
     5581 tggtgccaga tttaacggca gcaaggctat tcagcagttc gttcgggtca ataccgtcag
     5641 cgatcagcat ttcgcgatat tgctgcagtt tacgagtgcg ctcttcaact tcagcagcag
     5701 ccgcgctttc ttcttcgcga cgttcgttaa caacaacttc taatttttcc agcatttctt
     5761 ccagcgtttc aagtgtacat tctcttgcct gcgcacgaag agtacggatg ttgttcagaa
     5821 ttttaagtgc ttcgctcatt gtagtaatct caaacttata ttggggtggt ttgttgaggt
     5881 aataatagag ccttaaattc agttgtgcaa tagccaggaa tgtaaggaat tcaaaattgt
     5941 tctttatttt gtgccgccaa taaatatctt ttcataaaat tagccagaaa agacgcggca
     6001 tatagcccta tttgcaccga tgatttcgca gctcgtgagg ttaaaacttc ctgattcatg
     6061 tcacatttta tggggagatt atcgtaggct gacgaccttt cagtcttctg tattagtcgt
     6121 gtttacgaga attccctatt aagcgaatga tgaaaagtag aacagtcgca ataagagcag
     6181 ggacttagta ttgcactatc tcctggaggt caacagaggg ctattacttg cgcaacaggt
     6241 taaagattgt gaatagttac cagcagtcat ttacccgctt ataacaagcg aggcaatagt
     6301 aatgatagct cagaaggatt atgcaaggct tcgtaaggga gaacgcatat accacttctg
     6361 tgcatactgt tgagctgaaa aactgacgaa ttatgataaa ctccagccaa cgttatttca
     6421 tatcattgag ggcctgtggc tgatggcaca gctatatttc tactattccg caatgaatgc
     6481 gggtaagtct acagcattgt tgcaatcttc atacaattac caggaacgcg gcatgcgcac
     6541 tgtcgtatat acggcagaaa ttgatgatcg ctttggtgcc gggaaagtca gctcgcgtat
     6601 aggtttgtca tcgcctgcaa aattatttaa ccaaaattca tcattatttg ctgagattcg
     6661 tgcggaacat gaacagcagg caattcattg cgtactggtt gatgaatgcc agtttttaac
     6721 cagacaacaa gtatatgaat tatcggaggt tgtagatcaa ctcgatatac ccgtactttg
     6781 ttatggttta cgtaccgatt ttcgaggtga attatttatt ggtagccaat acttactggc
     6841 atggtccgac aaactggttg aattaaaaac catctgtttt tgtggccgta aagcaagcat
     6901 ggtgctgcgt cttgatcaag caggcagacc ttataacgaa ggtgagcagg tggttattgg
     6961 tggtaatgaa cgctacgttt ctgtatgccg taaacactat aaagaggcgt tacaagtcgg
     7021 ctcattaacg gctattcagg aaaggcatcg ccacgattaa taagaatttc tttactgaca
     7081 gggtgagcag ggcactttta tcctgtcagt tcgttttacg cacttcttcc gggctataca
     7141 cccttctcgg cagtttttta acgccgctat acgcctcaca gggctcttaa gcaccgacgt
     7201 tgacttgtga cctgtaaagt acaatatccc tgtgtttacg cgttatacat cgtcgcaaat
     7261 atgatgaagg ctaatgctgt cggtttatgg aaaagttgct ttgggtaaac aaaaaaacgg
     7321 ccccagaagg ggccgtttat gttgccagac agcgctactg attaagcgga ttttttcgct
     7381 tttttctcag ctttagccgg agcagcttct tttttcgctg cagtttcacc ttctacataa
     7441 tcacgaccgt agtaggtatc cagcagaatc tgtttcagct cggagatcag cgggtaacgc
     7501 gggttagcgc cggtgcactg gtcatcgaat gcatcttcag acagtttatc cacgttcgcc
     7561 aggaagtctg cttcctgaac gccagcttca cggatagatt tcggaatacc cagttcagcc
     7621 ttcagcgttt ccagccatgc cagcagtttc tcgatcttag cagcagtacg gtcgcccggt
     7681 gcgctcagac ccaggtggtc ggcaatttca gcataacgac ggcgagcctg cggacggtca
     7741 tactggctga atgcagtctg cttggtcggg ttgtcgttcg cattgtagcg aataacgtta
     7801 caaatcagca gggcgtttgc cagaccgtgc ggaatatgga actgggaacc cagtttgtgc
     7861 gccattgagt gacatacacc caggaaggcg ttcgcaaacg cgatacccgc gatagtcgct
     7921 gcactgtgaa cacgttcacg cgctaccgga tttttagacc cttcgtggta ggacgctggc
     7981 agatattctt tcagcagttt cagtgcctgc agagcctgac catcagagaa ctcagatgcc
     8041 agtacagaaa cataagcttc catggcgtga gttactgcgt ccagaccacc gaaagcacac
     8101 agggacttcg gcatgtccat aaccaggttg gcgtcgacaa tcgccatatc cggggtcagc
     8161 gcatagtccg ccagcgggta tttctgacca gtagcgtcgt cagttacaac cgcaaacgga
     8221 gtgacttcag aacctgtacc agaagtggtg gtgacagcga tcattttcgc tttcacgccc
     8281 attttcggga acttgtagat acgtttacgg atatccataa agcgcagcgc cagctcttcg
     8341 aagtgagttt ccggatgttc gtacataacc cacatgatct tcgcggcgtc catcggggaa
     8401 ccaccaccca gcgcgataat cacgtctggt ttgaaggagt ttgccagttc tgcaccttta
     8461 cgaacgatgc tcagggtcgg gtccgcttct acttcgaaga agacttcagt ttcaacgcct
     8521 gctgctttca gtacggaagt gatctgatca gcataaccat tgttgaacag gaagcggtca
     8581 gtcacgatga gcgcacgttt gtggccatca gtaatcactt catccagcgc gattggcagg
     8641 gagccacggc ggaagtagat agatttcgga agtttgtgcc acaacatgtt ttcagctcgc
     8701 ttagcaacgg ttttcttgtt gatcaggtgt ttcggaccaa cgttttcaga gatggagtta
     8761 ccaccccaag aaccacaacc cagagtcagg gaaggtgcga gtttgaagtt atacaggtca
     8821 ccgataccac cctgagacgc tggggtgtta atcaggatac gagccgtttt cattttctga
     8881 ccgaagtaag aaacgcgagc cggttggtta tcctggtcag tgtacaggca agaggtatga
     8941 ccgataccgc ccatagcaac cagtttctct gctttttcta ccgcgtcttc gaaatcttta
     9001 gcgcggtaca ttgccagagt cggggacagt ttttcatgtg cgaacggttc gctttcatca
     9061 acaacggtca cttcaccgat cagaatcttg gtgttttctg gtacagagaa gcctgccagt
     9121 tcagcaattt tataggctgg ctgaccaacg atagccgcgt tcagcgcacc gtttttcagg
     9181 ataacatcct gaacagcttt cagctcttta ccctgcaaca gatagccgcc gtgggttgca
     9241 aaacgttcac gtacagcgtc ataaacagag tcaacaacaa caacagactg ttcagaagca
     9301 cagattacgc cgttgtcgaa ggttttggac atcagtacag atgcaactgc acgtttgata
     9361 tcagcagttt catcgataac aactggagtg ttgcccgcgc ctacaccgat agctggttta
     9421 ccggagctgt atgcggcttt aaccatgccc ggaccaccag tcgcgaggat caggttgatg
     9481 tctgggtggt gcatcagtgc gttagacagt tcaacagaag gttgatcgat ccagccgatc
     9541 agatctttcg gagcaccggc agcgatagca gcctgcagaa cgatatcagc cgctttgttg
     9601 gtggcatctt ttgcacgcgg gtgcggggag aagataatgg cgttacgggt cttcagactg
     9661 atcagcgatt tgaagatagc agttgaagtc gggttagtgg tcggaacgat accgcaaata
     9721 ataccgattg gttcagcgat agtgatggta ccaaaagtgt cgtcttcaga cagaacacca
     9781 caggtttttt catctttata ggcgttgtag atatattcag aagcaaagtg gtttttgatc
     9841 actttatctt cgacgatacc catgccggat tcggcaacgg ccattttcgc gagtgggatt
     9901 cgagcatctg cagcagccag agcggcggcg cggaagattt tgtctacttg ctcttgagtg
     9961 aaactggcat attcacgctg ggcttttttt acacgctcta cgagtgcgtt aagttcagcg
    10021 acattagtaa cagccataat gctctcctga taatgttaaa cttttttagt aaatcatctg
    10081 ctcgaatacg agagtatagt cagtgcggtg atgatttgct taacctatga aaatcaaaag
    10141 cttactcgcg ctcacactca ctgtgattta ctaaaagagt ttaaacatta gagttattat
    10201 ctctaatgcg tcacttccag gtggcgtaag caagattact cacttctggg tactgattac
    10261 gtgatccaaa tcaaattttt gcaaagctga cacctttcag catcgctttt cgccattata
    10321 gctaacagtt aataaattgt agtatgattt ggtggctaca ttagcatgtt ttgcacaact
    10381 agataacaat aacgaatgat agcaatttta agtagttagg aggtgaaaaa tgctgtcaaa
    10441 aggcgtattg tcagcgcgtc ttttcaacct tatttatggc taacattatc cggcttttgc
    10501 ttcggagcta accgtgattc agaccttttt tgattttccc gtttacttca aatttttcat
    10561 cgggttattt gcgctggtca acccggtagg gattattccc gtctttatca gcatgaccag
    10621 ttatcagaca gcggcagcgc gaaacaaaac taaccttaca gccaacctgt ctgtggccat
    10681 tatcttgtgg atctcgcttt ttctcggcga cacgattctg caactttttg gtatatcaat
    10741 tgattcgttc cgtatcgccg ggggcatcct ggtggtgacg atagcgatgt cgatgatcag
    10801 cggcaagctt ggcgaggata aacagaacaa gcaagaaaaa tcagaaaccg cggtacgtga
    10861 aagcattggt gtggtgccat tggcgttgcc gttgatggcg gggccagggg cgatcagttc
    10921 taccatcgtc tggggtacgc gttatcacag cattagctat ctgtttggtt tctttgtggc
    10981 tattgcattg ttcgctttat gttgctgggg attgttccgc atggcaccgt ggctggtacg
    11041 ggttttacgc cagaccggca tcaacgtgat tacgcgtatt atggggctat tgctgatggc
    11101 attggggatt gaatttatcg ttactggtat taaggggatt ttccccggcc tgcttaatta
    11161 attcctttca aatgaaacgg agctgccatg ctccgtttac ttcgtcatta tttttacttt
    11221 gttcccgcgc agttatcaaa agcaaaagga ataggtaaaa tattcttctc aaattacagt
    11281 tagttataag gatttcctta actgcttctc ctcaccatca tgttattttc gccacatcat
    11341 aatcctgggc ttgctgaa
//
LOCUS       DBANUU010000137        11303 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000137 DBANUU010000000
VERSION     DBANUU010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.138"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..204
                     /locus_tag="ACIE68_003786"
     CDS             <1..204
                     /locus_tag="ACIE68_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HFX5910034.1"
                     /translation="PARINKLIKRLESYGLNFSVITCPRKTSLYEKLSIPFVYLLFQW
                     SLPHLHSDGMTVVIRPLQPPHTN"
     gene            238..1305
                     /gene="pilL"
                     /locus_tag="ACIE68_003787"
     CDS             238..1305
                     /gene="pilL"
                     /locus_tag="ACIE68_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="HFX5910035.1"
                     /translation="MKKNCLTLILPSLLSGCAGISGSNNVAPSDTPALVFVDGQISES
                     AGVLALTQQRLSPPKITPPPRVSVPETVKTAQPVSESRPLPSPTISQNFSRLIITGKP
                     GSAPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLN
                     RLLEEQHLWGHLDWNNKTLTVTTSAAAPAQDTATTISPAAMVTTSTYPESPTTANSQN
                     KPRNPFRGNSVSSSTPAATPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLREN
                     IIKWAEETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKP
                     LYAQASRMQCIITVSDTRDGR"
     gene            1305..1742
                     /gene="pilM"
                     /locus_tag="ACIE68_003788"
     CDS             1305..1742
                     /gene="pilM"
                     /locus_tag="ACIE68_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFX5910036.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQKMSETTNAQQYASASVWASQIL
                     MIANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETL
                     RSKSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
     gene            1756..3438
                     /locus_tag="ACIE68_003789"
     CDS             1756..3438
                     /locus_tag="ACIE68_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFX5910037.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSA
                     HAREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQE
                     LGQRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTS
                     TQPLTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSS
                     SSSVSSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGR
                     YWLSASSSTLTVTDTPAVQEAVARYVDDQNSIMNRQVALNVQVLSVSNTRNEQFGLDW
                     NLVYKSLHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDV
                     SVVTSQESTVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLL
                     PLIQKTGNLQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLV
                     VTGFDQNNTTTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
     gene            3431..4726
                     /gene="pilO2"
                     /locus_tag="ACIE68_003790"
     CDS             3431..4726
                     /gene="pilO2"
                     /locus_tag="ACIE68_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFX5910038.1"
                     /translation="MADEDINPVVLLADPKVNHRVWAACLKWSPVVKKQRVPSHQKHK
                     PHVKSRRLTSLKVTVGSRSSRGKISRITGTGILARPERNHYFSLALAFCSWVRNGYGV
                     FRYSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLTMNEEPPEKWQVVSPLEHP
                     DNWESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAAGAGTVFWMTQPEPDVVPTP
                     EEIAARARLQFKKPDPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTC
                     TPETFTLIYERQPGGTIEGFLARSKEIFNVIPDFNLKDGARLASVTRPLPSLPRRDEA
                     VPAPSEQLMRVFTWFQKKQLTPAINEIAIPEPLPGNDGEPAPVQKWKEYQFSLSTPVN
                     PDELFPLFQDTGVRLSNIHFELNGGTFSYSSEGHIYASR"
     gene            4713..5165
                     /gene="pilP"
                     /locus_tag="ACIE68_003791"
     CDS             4713..5165
                     /gene="pilP"
                     /locus_tag="ACIE68_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFX5910039.1"
                     /translation="MRPGKLLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQA
                     KVQGAQLQKQLEESDVTSSSETVSGFSGVTASLPSVSEQPTSKNRKELPVIMEINGKD
                     KRLNAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
     gene            5176..6729
                     /locus_tag="ACIE68_003792"
     CDS             5176..6729
                     /locus_tag="ACIE68_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFX5910040.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLKLY
                     PDINPKVVSLSELQEAGQDEKNRGPKERIIHLKDLADVSESEKKVLSYFETARKLGAS
                     DIHFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQD
                     ARLSPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLN
                     IMLRRPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTP
                     IICDKSDEDAVKLAWSRAISSAMRLDPDAIMEGEMRDLISMMSTTYAAQTGHIVLTTL
                     HTNSALGIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWEKRAPELSDD
                     ERDYLERHCNKDSLCSTDNIWFRNHHGCSECNHDVIINGRKRGEIGKGLTGRTVIAEV
                     IEPDNRLFQILKTRGKVAARKYWLENMKGISRVEHLLRRINEGLVDPLEADRIIPLDE
                     DERLSIDDV"
     gene            6742..7839
                     /locus_tag="ACIE68_003793"
     CDS             6742..7839
                     /locus_tag="ACIE68_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFX5910041.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTA
                     LEQMRDAWTDFGRKWHPFAELATDCIESLRENSGENSLEYTLSLWVPQEEAAVISAGI
                     RSGSIVDALQFATTLTDAKEQIHQAIWQMAIYPVGLLIMMTGTLYVLNTELIPELSKI
                     SSPDSWSGALGFLYGLSVFVDNYGAICAVLFAVITGLISWSLPNWKSPDSVRTFADKI
                     MPWSIYQDIQGATFLLNMAALLKAKMTTLNSLNILQEFASPWLSTRLDSIIYRVRQGD
                     HLGLALRQCGYQFPSREAANFLSLLQGDGATELISNYGQRWLSQTLQRVKKRANVIRL
                     IMLIFLVMSLMLLVFAIMDIQSISDNSMGNF"
     gene            7857..8471
                     /locus_tag="ACIE68_003794"
     CDS             7857..8471
                     /locus_tag="ACIE68_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFX5910042.1"
                     /translation="MLVENINTTLTGNNKKNEPHDKGWAILEQGTIALVVLFVIVVVL
                     GSLYALRTRANVATETANIQTIITSAQSLLKGSDGYTFTSSAKMTGALIQMGVIPSGM
                     TVQGDKTSGTATLYNAWGGAVTVAPASTSGFNNGFTVTYDKVPQDACIQIATRISKTG
                     LTNGITLNSTAHSDGKVTTEEASTQCKADNGSTGTNKLIFTING"
     gene            8481..9041
                     /locus_tag="ACIE68_003795"
     CDS             8481..9041
                     /locus_tag="ACIE68_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFX5910043.1"
                     /translation="MSGGTVPLRLISLILLFSLIPPARASYVAPKQIWTNKWDNCFAA
                     AGARYQIEPLLLKAISAGESSLRPGAININKDKKTGKASSTDYGLMQINSTHIPKLIN
                     MGVIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYA
                     NKIWRIYRDMKGICLPGQGGRQCRQS"
     gene            9026..9682
                     /locus_tag="ACIE68_003796"
     CDS             9026..9682
                     /locus_tag="ACIE68_003796"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFX5910044.1"
                     /translation="MQTIVGISLSPVYILPLMFTFSIIGRTLLFRVRYFLSDTGHLWY
                     KTHPAVLSGIWLYSIAVALIILSSSPLLYRIHAVLILSFILQMAVTDALTGLLPGTFT
                     RRFLIAGMLSQITTDIWWFRTTEFATAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITA
                     WSGLYNAIWCVLLGTGGFVLWHSTWCIKGHKEGPLGPWLCFGHVLLLLDNLYQPLWVI
                     "
     gene            9682..10974
                     /locus_tag="ACIE68_003797"
     CDS             9682..10974
                     /locus_tag="ACIE68_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFX5910045.1"
                     /translation="MKKYDRGWASLETGAALLIVMLLIAWGAGIWQDYIQTKGWQTEA
                     RLVSNWTSAARSYIGKNYTTLQGSSTTTTPAVITTTMLKNTGFLSSGFTETNSEGQRL
                     QAYVVRNAQNPELLQAMVVSSGGTPYPVKALIQMAKDITTGLGGYIQDGKTATGALRS
                     WSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGS
                     NNLNNVGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGWLITR
                     NSKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLERT
                     AVAGASCSPNGLVGRDNTGAILSCQSGTWGTIGGKLKVTQLSTTGYLGQFDFCAIARM
                     GNAEDAHYCQVVESPAGSRKWYKYEHKTGCIASCVTLN"
     gene            complement(10971..>11303)
                     /locus_tag="ACIE68_003798"
     CDS             complement(10971..>11303)
                     /locus_tag="ACIE68_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin, shufflon protein A"
                     /protein_id="HFX5910046.1"
                     /translation="TSGSLNGSYTNLGSHRGSFSGRNSGGSTLFIYASGGNGGSAGGA
                     CANTSRLQGYVGGTLISVNASNNPAYGKTAFISFAVPAGTSYQITSYPTENTSCGAGV
                     FSVFGYQT"
BASE COUNT         3233 a         2683 c         2603 g         2784 t
ORIGIN      
        1 cctgcaagaa taaataaact tataaagaga ctggaatctt atggtttgaa tttctctgtt
       61 attacatgcc cacggaaaac aagtctgtat gaaaaattat caattccttt tgtatatctc
      121 ttatttcagt ggtctctccc gcatttgcat tcggacggaa tgacagtcgt cattcgtcct
      181 ctccagccgc ctcacaccaa ttaacaaaca cgtatatacg aacacggagt ctccgttatg
      241 aaaaaaaact gtctgacact tatactgccg tcattattgt ccggctgtgc cggaatttcc
      301 ggcagcaata acgtggcacc atcagatacg cctgctctgg tttttgttga cggacaaatc
      361 agtgaaagtg ctggcgttct ggcacttacg caacaacgtc tttctccacc taaaataact
      421 ccccccccga gagtcagcgt gccagagaca gtaaaaacag cacagcctgt tagcgaatcc
      481 cgaccgttac cttcaccaac aataagtcag aatttctccc ggttaatcat tacaggcaag
      541 cctggtagcg cccctgtaac actatcgccg acggcccgta accggaccgt cagtcagtgg
      601 ataaaaacat tactaccgga aggatggaaa ttcagacaag agaatgcagc cacgcccaag
      661 ctgaatacca aactggtttc atggtctgcc aatgatcagt ggacccgttc tctgaaccgg
      721 ttgctggaag aacaacacct ctggggccat ctggactgga ataataaaac gttgaccgtc
      781 acaacatcag ctgctgcacc ggcacaggac acggcaacca ctatctcgcc agcagccatg
      841 gtaaccacat ccacttaccc tgaatctcca acgacagcaa atagccaaaa caagccacga
      901 aatccattca ggggaaacag cgtatcttcg tcaacgcccg ccgccacccc gactccaacc
      961 ggctccacag tcaagtctat cccgctcatg accggaacgc ctgttaaacc ggtatctcag
     1021 ggtaaagagt ggcgtgcgcc ggcaggcacc acattaagag agaacattat caagtgggca
     1081 gaagaaacaa aatgcgaaag catggcatca acgcactgga tggtcatatg gccactttct
     1141 gtaaccgatt accgtctgga tgcgccttta atgttccggg gatcatttga atcagccatg
     1201 gagcaggtat ttgagttata tcggactgcg caaaaacctt tatacgcaca ggcttctcgc
     1261 atgcagtgca tcattacagt ttctgataca agggatggtc gctaatggga tggctggtca
     1321 tggccgtcgg cctgaccatt ctgattatca ctggtagcta ccagaatcag aagatgtctg
     1381 aaaccacaaa cgcccagcaa tatgcaagtg cgtctgtatg ggcaagtcag atactaatga
     1441 ttgctaacag aatcaatgat atcagatacg tatcaggcca gcaggacgga gtaatttcct
     1501 cagataaact ggctctccct gtcactccgg actcccgaat taaacatcag ttacaacagg
     1561 ggcgtctgtg ggtatggatg cctgaacaac caggtcttgt tgaaactttg cgttcaaaaa
     1621 gtcgtggttc tgcactaata gggatcttcc aaaacgggca actcacctgg ctatcaggaa
     1681 ctgcaaccgg tctgacaccc ccggcaggca ttactgctgg ttctgtcgtc tacgttaact
     1741 aatctggata tattcatgaa aaaatcacac cagcgttcaa tgaagctggc ggtgctcccc
     1801 tgcatgatcg ccgtcgctct ttccatctcc ggatgtactt tcagcgaaat caacaaaatg
     1861 cagaaaaaag cacaggaaga ctcagcacat gcacgggaaa aggtatcagc cctttcggcc
     1921 cgtaaatcgc aggctcttac ctggctcgat aatcaatgga taaaccctgt tccggtcgct
     1981 caggtatcaa gagagaaaaa acaaacagct ccggcctgct acatcacgca ggcaagaaaa
     2041 ggagagatca ctctgcagga actggggcaa cgtattactg ccgtatgcgg catccctgtg
     2101 atcatcacgc ctgacgcagc caattcaact cttgaaggag gcgctacccg ccagatgaca
     2161 ggaacactac cagcaccaga tgaaaatggg cgtctaccgt taagcagcct gggcagcaca
     2221 acaatgacta cctccactca gccattaacg ctgaataacc tcatgtggca gggagatatc
     2281 aatggtcttc tggatctgat ggccagccgg agtggtctgt actggcgcat ggataatggt
     2341 cggattgtat tctatctgac tgaaaccaga acgtatccac ttcatatgct gaacaccaaa
     2401 accagcagca gttccagtgt cagctctggc tcaacaagca caatgggggc aacaggaggc
     2461 caggataact cagcatccgg tgatgcaacg tcctctcaga gcacaaccgt tggtcaggaa
     2521 tacgatctgt atgaagacat ccggaaaact attgaagcaa tgctgacacc agaaaaaggc
     2581 cgttactggt tatctgcatc gagctcaacg ctgactgtca ctgatactcc agctgtccag
     2641 gaagccgtcg cacgatatgt ggacgaccaa aacagtatta tgaaccgcca ggtagccctg
     2701 aacgtacagg ttctgagcgt cagcaatacc agaaacgaac agttcggtct ggactggaac
     2761 cttgtttata aatcgctaca ttccgccgga gcaacgttga acaatgcaag cggagatttt
     2821 acaggcgcta catctgcagg cgtatcaatt ctggatacgg caacagggaa tgccgccaaa
     2881 ttcagcggtt ccagtcttct gattaaagcg ctgagtgaac agggcgatgt cagtgttgtg
     2941 acttcacaag aaagcactgt cacaaacctg acgccggtac ctatccagat ggcagatcag
     3001 acggtttacg tcgcccagtc agcaacaaca acgactacgg atgtaggagc aacaacaaca
     3061 ttaacgccgg gcatgatcac caccggattc aatatgaccc tgctgccttt aattcagaaa
     3121 acgggcaatc tccagttgca gatgaatttt aatctgtcag atcccccaac aatccgtagc
     3181 tttacgtcaa aagacggaaa cagttacatc gaaatgccgt ataccaaact gcgttcactg
     3241 agccagaagg tcaatctgaa agaagggcaa tcacttgtcg ttactggttt cgatcagaac
     3301 aatacgacga caagtaaagc cggtacgttt acgccagcaa atccattatt tggtggttca
     3361 caaaccggga aaaatgaacg cagcacgctt gtaatcatca ttaccccgac tttcccgtca
     3421 ggaggcaaca atggctgatg aagatatcaa cccggttgtg cttctcgcag atcctaaagt
     3481 aaatcatcgc gtgtgggcag cctgtcttaa atggagtcct gtcgtaaaaa aacagcgagt
     3541 cccttctcat cagaaacaca agccccatgt aaaatccaga cggcttacct ctctgaaagt
     3601 gactgtaggc agcagaagtt cccgtgggaa gatcagccgc attaccggca caggaatact
     3661 ggccaggccg gagcgcaatc attatttttc ccttgctctg gctttctgct catgggtgag
     3721 aaatggatac ggcgtcttcc ggtacagcga taaggagctt ttattccttg cctccattaa
     3781 tggccagcct gccgttatgg ctgatctgtc aggaaatgat gctgatgttg ctcaaaaggt
     3841 cagtcttttt ctgacgatga acgaagagcc ccccgaaaaa tggcaggtcg tatctcctct
     3901 ggaacatcct gataactggg aaagtattat tacccggtta tcatcagcag atctccgccg
     3961 ttgcaaactt actgtcggca atcgcagcaa attcacactg cctgccgtac tgtttttagt
     4021 agcagccggc gcaggcactg tattctggat gactcagcca gagccggatg tcgtaccgac
     4081 gcccgaggag atagctgcac gggcaagatt gcagtttaaa aagcccgatc caccgccaga
     4141 actcccgcat ccatgggcat cacaacctgt aatcagtgat tttctgaaag cctgcgcgga
     4201 ccttcgaaaa ccatcaccgg tcgcgctaga aggctggaaa cttaccggtg gaacatgtac
     4261 tcccgagaca ttcacactga tttacgaacg acagccagga ggaactattg aaggctttct
     4321 ggcaagaagc aaagaaatct ttaatgttat tcctgacttt aaccttaaag atggtgcacg
     4381 cctggcttct gtaacccggc cactgccttc cctgccccgc agggatgagg cagtccctgc
     4441 cccctcagaa caactgatgc gggtgttcac gtggtttcag aaaaaacagt taacgccggc
     4501 cattaatgaa atagccatac cggaaccatt accaggtaac gatggtgaac cagcccctgt
     4561 tcagaaatgg aaagaatatc agttcagttt atcaacgcct gtaaatcctg atgagttgtt
     4621 ccctttattt caggataccg gcgttcgtct gagcaatatc cattttgaat taaacggcgg
     4681 tactttcagt tattcctcag aaggtcatat ttatgcgtcc aggtaaatta ctcattattc
     4741 catccatatt tttcttctcc ggtttttcat ttgcaacaac acaacctctt gtcacaatag
     4801 gagaacttga agcacaacaa aaccggaata ttcttttaca ggcaaaagtt cagggcgcac
     4861 agctacagaa acaactcgaa gaatcagatg ttacttcatc atctgaaact gtttcaggtt
     4921 tctctggtgt gactgcctct ttgccatcag tttcagagca gccgacatct aaaaacagaa
     4981 aggaattgcc cgttattatg gaaataaacg gcaaagataa acgtcttaat gccgttcttc
     5041 gcatggctga tggacgtcag acaagcgtca ccactggcag ccagcttcca ggcacatccg
     5101 tcacagtgaa gtcgatctca ttgtctggcg tcacgttaag tgatggcacc acccttactt
     5161 tctgagtaac gtgatatgga taaatataat ctgaaggatg ctctcctttt tatatccagg
     5221 ggagacaccc atgaaattct tatagaaaca aaccagcgga caaggcctga cgtacaaagt
     5281 aatctccagg agctgctaaa attatatccg gatatcaacc caaaagtagt ctcgttgtcg
     5341 gagttacagg aggcaggtca ggatgagaaa aacagaggcc cgaaagaacg aattattcat
     5401 ctgaaagacc ttgctgatgt cagtgaaagt gagaagaaag ttctatctta ttttgaaact
     5461 gccagaaaac ttggtgcttc agatattcat ttcttaattt cagagtctat tttcaaagtt
     5521 cgcatgagaa tattcggtga actgcagact gtagatgaag atcaaccagc actgggctat
     5581 tcactttgcg caacagccat tctgtcaatg gctgatgtca ctgaaaccag tttcttccct
     5641 caaagggagc aggatgcgcg cctgtcccca caattaatgc gaaaaatagg aatatttggt
     5701 gcacgctaca gtcacagacc tacgggagac ggtctgattg cagttatgcg cctgataccc
     5761 gatgacggag ataaagttcc cacgtttaaa caactgggct ttatcccgga acaaatccgg
     5821 ttgctgaaca taatgctgcg ccgcccggaa ggaaaaatag tcctgtcagg tccaaccggt
     5881 tccggtaaat cgacaacgtt acgcagcgcc tgccgtgtat atcttgacga taatcagggg
     5941 cgacacctgc taacaatcga agatccgctg gaaggacaga tactgggagc aacacagact
     6001 ccgattatct gcgacaaatc cgacgaagat gccgtcaaac tggcatggag tcgggctatt
     6061 tcatcggcaa tgcgacttga ccctgatgcc atcatggaag gggaaatgcg cgatcttatc
     6121 tcgatgatgt caaccaccta cgcggcacag accgggcaca tagttctgac gacattacat
     6181 acaaactcag ctctgggtat accggagcga atgattacga tgggtatgaa tgccgatctt
     6241 atttgcgatg cgcaattact gattgggatg atcagccagc gccttgtacc aactctgtgt
     6301 ccctcctgcc gtattccatg ggaaaagcga gcacctgaac ttagcgacga cgagcgtgat
     6361 taccttgagc ggcactgcaa taaagattca ctctgcagta cagataatat ctggttccga
     6421 aatcatcacg gatgctctga atgtaatcac gacgtaatca ttaacggcag aaagcgcggt
     6481 gaaataggta aaggcctcac cggcaggact gtaattgctg aggtgataga acctgataac
     6541 cgcttatttc agattctgaa aacacgcggc aaagttgcag cacgtaaata ctggctggaa
     6601 aacatgaaag gcatcagtcg tgtagagcat cttcttcgcc gtatcaatga aggtctggta
     6661 gaccctcttg aagccgacag aatcatccct ctggacgaag acgagcgcct cagcatcgat
     6721 gatgtctaac aggagaacaa catgcgtgaa atgaatttca gtcaacggct gaggcgtttc
     6781 attgtcagaa aaacgttcag cgccccctac agggtgcagt tttacgaagc cctgcgtttt
     6841 ctgctggaaa ataaacagcc attaaagacc gcactggaac agatgcggga tgcctggaca
     6901 gactttggac gaaaatggca tccctttgct gaactggcca cagactgcat tgagtctctt
     6961 cgtgaaaaca gtggcgaaaa ctctctggaa tatactctta gtttatgggt tcctcaggaa
     7021 gaggccgcag taatcagcgc cgggataaga agtggttcaa ttgtggatgc actgcaattc
     7081 gccaccactc ttacagatgc aaaagaacaa atccatcagg ctatatggca aatggctatt
     7141 tatccggtgg gattgttaat catgatgacg gggacattat atgttctgaa tacagagcta
     7201 atacctgaac tgagcaaaat aagctccccc gattcatgga gtggtgctct tggttttctt
     7261 tatggattat ctgtttttgt cgataattac ggcgctatat gtgccgttct ttttgcagtg
     7321 atcacaggtt taatatcatg gtctctgcca aactggaaaa gccctgacag tgttcgtact
     7381 tttgccgaca agataatgcc atggagtatt tatcaggata tacagggagc aacatttctc
     7441 ctgaatatgg ctgcacttct gaaagccaaa atgacgacgc tcaactcgct aaacattctt
     7501 caggagtttg catcaccatg gttaagcaca cgtcttgaca gcattattta ccgcgtccgc
     7561 cagggggatc atcttggact ggctttgcgt cagtgcggtt atcagttccc atccagggaa
     7621 gcagccaact tcctgtcttt attacaggga gatggcgcaa cagagctaat cagcaattat
     7681 ggtcaacgat ggcttagtca aacacttcaa cgtgtaaaga aacgggcaaa cgtgattcgt
     7741 ttaattatgc tgatatttct tgtcatgtct ctcatgttac tggtttttgc cattatggat
     7801 attcagtcta ttagtgacaa cagcatgggt aacttttaaa caaaacgagg atatttatgt
     7861 tagtagaaaa tattaatacc actttaactg gaaataataa gaagaatgag cctcatgata
     7921 aaggatgggc aattttggag cagggaacaa tagcgctcgt tgttcttttt gttatagtcg
     7981 ttgttctggg tagtttgtat gcgttgcgta caagagccaa tgtggcaact gaaaccgcaa
     8041 atatacaaac aattatcaca agtgcccaga gcctgctaaa aggtagcgat ggatatacat
     8101 ttaccagcag tgccaaaatg acaggtgccc tcattcagat gggtgtcatt ccctctggga
     8161 tgaccgtaca gggcgacaaa acatctggta cagcaacgct ctataacgca tggggcggtg
     8221 ctgtaactgt tgccccggct tcaacatcag ggttcaacaa cggttttacc gttacctatg
     8281 ataaagtccc acaggatgcc tgtatccaga ttgccacacg aatcagtaaa actggtttaa
     8341 ctaacggcat taccctgaac agtactgctc acagtgatgg aaaagtcact actgaagaag
     8401 ccagtacgca atgcaaggca gataatggca gcactggtac aaacaaactt atcttcacca
     8461 tcaatggtta aggagtttta atgtcagggg gtacagtacc cctcagactt atttcattaa
     8521 tcctgctttt ttctcttatt ccacccgccc gagcttcata cgtagctccc aaacagatct
     8581 ggaccaacaa gtgggacaat tgctttgccg ctgccggcgc gcgctatcaa attgaaccat
     8641 tattattgaa ggctatttct gccggggaat cttcgttgag gccgggcgca attaatataa
     8701 ataaggacaa aaaaacaggt aaagccagta gcacggatta tgggttgatg caaataaact
     8761 caacccatat tccaaagctg ataaatatgg gcgtaataaa aaagtctgag gatttaatta
     8821 ccaaaccatg ccttaatatc catataggaa gctggatact ggcaagacat tttcagatat
     8881 gtggcgtcag ctggaattgc cttgggtcat acaatgccgg attccgtaag gacagacatg
     8941 aaacaagaga gcaatatgcc aataaaatat ggagaattta tcgtgacatg aaagggatat
     9001 gtcttcccgg acagggaggc aggcaatgca gacaatcgta ggcataagtc tatcccctgt
     9061 ttatatactg ccattgatgt tcacattttc cattattgga cgaactctgc tattcagagt
     9121 tcgttatttt ttatctgata cgggacatct ctggtataag acacaccctg ctgttctgtc
     9181 aggaatatgg ctatacagta tagctgtggc gttaattatt ttatcatcat caccactgct
     9241 ctaccgtatt catgctgtcc ttattctttc gttcatactg caaatggcgg taacggatgc
     9301 actgacaggt ttattaccag gaacattcac ccgacgtttt ttaattgcag gaatgctatc
     9361 acaaatcacg actgatatat ggtggttccg tacaacagaa tttgccactg ctgcaattgt
     9421 cttattctgc cttcataaat tagttaaccg ccatcgtctt aatattggca ctggagacct
     9481 gtggcttatt gcaggtatta ccgcatggtc agggttatat aacgccatat ggtgtgtgtt
     9541 gctgggcaca ggaggatttg tcttatggca ttcaacatgg tgcataaaag ggcataaaga
     9601 aggccctctg ggtccatggc tatgttttgg ccatgtcctt ttgctgctgg ataatcttta
     9661 tcaaccacta tgggtaatct gatgaaaaaa tatgaccgag gctgggcatc gctggaaact
     9721 ggcgcggctt tactgattgt tatgctcctg attgcctggg gagcgggcat atggcaggac
     9781 tatattcaaa caaaaggatg gcaaactgaa gcacgtcttg tcagcaactg gaccagtgcg
     9841 gcccgctctt atatagggaa aaactacacg actctccagg gcagtagtac cacaacaact
     9901 cctgccgtta tcacgacaac catgctgaaa aataccggct ttttgtccag cgggtttact
     9961 gagacaaaca gcgaggggca gcggttacag gcatatgtgg ttcgaaacgc ccaaaacccg
    10021 gaattactac aggcaatggt tgtatccagt ggtggcacac cttatccagt gaaagcactt
    10081 atccagatgg ctaaggatat taccactggt cttggtggat atatccagga cggcaaaaca
    10141 gccacaggtg cattacgttc ctggtcagta gctttaagta attatggtgc caaaagcggt
    10201 aacgggcata ttgccgtatt gttatcgaca gatgaactta gtggtgcagc tgaggacact
    10261 gatcgtcttt acagattcca ggtcaatggt cgccctgact taaacaaaat gcacacggcc
    10321 attgatatgg gatcaaataa cctgaataac gttggggcag taaatgccca gacaggtaat
    10381 ttcagcggca atgtgaatgg tgtaaatggc actttcagcg gtcaggtaaa aggcaatagc
    10441 ggaaactttg acgtaaatgt caccgctggc ggtgatatca gaagtaataa tggttggtta
    10501 attactcgta acagtaaagg ctggctcaat gaaactcacg gtggcggatt ttatatgtcc
    10561 gatggatcat gggttcgaag tgtaaacaac aagggcatct ataccggcgg tcaggtgaaa
    10621 ggcggtactg ttcgggctga tggtcgcctt tatactggtg aatacttaca actggaaaga
    10681 actgccgttg ctggcgcatc atgttcgcct aacggccttg taggccgcga taatacaggg
    10741 gcaatacttt cgtgccaatc cggtacgtgg gggacaatag gtggaaaact caaagttact
    10801 cagctttcca ccacaggtta tctggggcaa ttcgacttct gtgccattgc cagaatgggc
    10861 aacgcagagg atgcccacta ctgccaggta gttgagagcc cagcaggttc acgcaaatgg
    10921 tacaaatacg agcataagac aggttgtatt gcgtcgtgtg taacgctcaa ttaagtttga
    10981 tatccaaata ctgaaaatac cccggcacca catgatgtat tttctgttgg ataggatgtt
    11041 atctgatagg aagtacctgc aggtacagca aagctgataa aggctgtttt tccatatgca
    11101 gggttattgc tggcattcac gctaattagc gtcccaccaa catatccctg cagtcgggat
    11161 gtatttgcac atgcacctcc ggcagatcct ccattacctc cagatgcata aataaacaat
    11221 gtactgcccc ctgaattccg ccctgagaat gaacctctat gtgaacctaa gtttgtgtaa
    11281 gaaccattga gcgaaccaga agt
//
LOCUS       DBANUU010000138        11294 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000138 DBANUU010000000
VERSION     DBANUU010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.139"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            178..1749
                     /gene="garD"
                     /locus_tag="ACIE68_003799"
     CDS             178..1749
                     /gene="garD"
                     /locus_tag="ACIE68_003799"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFX5910047.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNIGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1898..2233
                     /gene="prlF"
                     /locus_tag="ACIE68_003800"
     CDS             1898..2233
                     /gene="prlF"
                     /locus_tag="ACIE68_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFX5910048.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2233..2697
                     /gene="yhaV"
                     /locus_tag="ACIE68_003801"
     CDS             2233..2697
                     /gene="yhaV"
                     /locus_tag="ACIE68_003801"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFX5910049.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2752..3561)
                     /gene="agaR"
                     /locus_tag="ACIE68_003802"
     CDS             complement(2752..3561)
                     /gene="agaR"
                     /locus_tag="ACIE68_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFX5910050.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPTDSLEGLRKAGVEVILVGE"
     gene            3810..5090
                     /gene="kbaZ"
                     /locus_tag="ACIE68_003803"
     CDS             3810..5090
                     /gene="kbaZ"
                     /locus_tag="ACIE68_003803"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFX5910051.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5113..5586
                     /gene="agaV"
                     /locus_tag="ACIE68_003804"
     CDS             5113..5586
                     /gene="agaV"
                     /locus_tag="ACIE68_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFX5910052.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5597..6376
                     /gene="agaW"
                     /locus_tag="ACIE68_003805"
     CDS             5597..6376
                     /gene="agaW"
                     /locus_tag="ACIE68_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFX5910053.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6366..7244
                     /gene="agaE"
                     /locus_tag="ACIE68_003806"
     CDS             6366..7244
                     /gene="agaE"
                     /locus_tag="ACIE68_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFX5910054.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7262..7696
                     /gene="agaF"
                     /locus_tag="ACIE68_003807"
     CDS             7262..7696
                     /gene="agaF"
                     /locus_tag="ACIE68_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFX5910055.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7693..8826
                     /gene="nagA"
                     /locus_tag="ACIE68_003808"
     CDS             7693..8826
                     /gene="nagA"
                     /locus_tag="ACIE68_003808"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFX5910056.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLCIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTSPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEKVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            9177..10331
                     /locus_tag="ACIE68_003809"
     CDS             9177..10331
                     /locus_tag="ACIE68_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFX5910057.1"
                     /translation="MPKNYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10344..11204
                     /gene="kbaY"
                     /locus_tag="ACIE68_003810"
     CDS             10344..11204
                     /gene="kbaY"
                     /locus_tag="ACIE68_003810"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFX5910058.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2710 a         2916 c         2993 g         2675 t
ORIGIN      
        1 aaagcacaga tttgcgcttt accttgccgg gcggcactgc aatccctgaa atgattgaca
       61 ttgatcacat ttctgcgttt aaactcctga cattcttatt tcacccaatg aagtcattta
      121 tttttaaatg agaccaggtc ctcattttaa taacccctgg ctggagaata ttgcacaatg
      181 gccaacatcg aaatcagaca agaaacgcca actgcgtttt atataaaagt tcacgacaca
      241 gataatgtgg caattattgt taatgataat ggcctgaaag caggaacgcg ttttccggat
      301 gggctggaat taattgaaca tattccccag gggcataaag tcgcattgct ggacattccg
      361 gctaatggtg aaattattcg ttatggcgaa gtgattggtt acgccgtgcg cgcaatccca
      421 cgcggaagct ggatcgacga atcaatggtt gtactaccgg aagcgccgcc gttacacacg
      481 ctgccactgg caaccaaagt cccggaaccc ttaccgccgc tggaaggata cacctttgag
      541 ggctatcgca atgccgatgg cagcgtgggc accaaaaacc tgctcggcat caccaccagc
      601 gtccactgtg tggcaggcgt ggtggactac gtagtaaaaa tcattgaacg cgatctgcta
      661 ccgaaatacc cgaacgtcga tggcgtggtg gggctgaatc atttgtacgg ttgtggcgtg
      721 gcgattaacg caccggcggc agttgtgcct attcgtacca ttcacaatat ttcgctgaac
      781 cctaactttg gcggcgaagt aatggtgatt ggcctgggtt gtgaaaagtt gcagcctgag
      841 cgcctgctga ctggaacgga tgatgtgcaa gctattccag tagaaagcgc cagcattgtc
      901 agtttgcagg atgaaaagca tgtcggtttt cagtccatgg tcgaggatat tttgcaggtc
      961 gccgaacgcc atctacaaaa actgaatcaa cgtcagcgag aaacctgtcc ggcttcagaa
     1021 ctggttgtcg gcatgcagtg cggtggcagc gatgcctttt ctggcgtaac ggcaaacccg
     1081 gcggttgggt atgcgtctga tctactggtg cgctgcggcg caacggtgat gttctcagaa
     1141 gtcacggaag tgcgtgacgc gatccatctg ctgacaccac gcgcagtgaa cgaagaggtc
     1201 ggcaaacggc tgctggagga gatggagtgg tacgataact atctcaatat aggaaaaacc
     1261 gaccgcagcg ccaacccttc gccgggcaac aagaaaggcg gtctggcaaa cgtggtagag
     1321 aaggcactcg gctccattgc taaatcgggt aaaagcgcaa ttgttgaagt gctgtcgccc
     1381 ggtcaacgcc cgactaaacg cggattaatt tacgccgcga cgccagccag cgattttgtc
     1441 tgtggcacgc aacaggtggc ttcgggtatc accgtgcaag tgtttacgac cggtcgtggt
     1501 acgccgtacg gcctgatggc ggtacccgtc attaaaatgg cgacccgcac cgagctggcg
     1561 aaccgctggt ttgatttaat ggatattaac gcgggcacta tcgccaccgg cgaagaaacc
     1621 attgaagagg tgggctggaa gttgttccac tttattctcg acgtcgccag cgggaagaag
     1681 aaaaccttct cggatcaatg ggggctgcat aaccagctgg cagtgtttaa cccggcaccg
     1741 gtgacctgat ttcttttgga acgtccctcg caaaacatgg ccttagtgcc atgtttttat
     1801 tgtttaaagc ccccacgtcc attaataatg catttgcatt accttagttc aagcttataa
     1861 tttgagcaga aaacaggctg taaaaggaca gtgaatcatg cccgctaatg ctcgctctca
     1921 cgctgtactg accactgaat caaaggtcac gatacgcgga caaacaacta tccccgcgcc
     1981 agtgcgtgag gccttaaaac tgaagccagg ccaggacagc attcattacg aaattctgcc
     2041 tggtgggcaa gtctttatgt gccgactggg agatgaacag gaggatcata ctatgaatgc
     2101 atttttgcgt tttctggatg cagatatcca gaacaacccg caaaaaactc gtccattcaa
     2161 cattcaacaa ggaaagaaac ttgtcgctgg catggacgtc aacattgatg atgagattgg
     2221 cgacgacgaa taatggattt tccacaaagg gttaatggtt gggcgctata tgctcatccc
     2281 tgttttcagg aaacctacga tgctttagtt gccgaagtcg aggcattaaa gggaaaagat
     2341 cctgaaaatt atcagagaaa agctgccaca aagttattgg cggtggtcca taaagtgatt
     2401 gaggagcata tcacggtcaa tccatcatca ccggcattcc gtcatggcaa gtcgttaggc
     2461 tctgggaaaa ataaagactg gtcacgggta aaatttggtg ctggtcgcta tcgtctcttc
     2521 tttcgttaca gtgaaaaaga gaaagtcatc attctgggat ggatgaacga tgaaaacacg
     2581 ctgcgcacct acggtaaaaa aacagatgcc tataccgtat tcagtaaaat gttaaaaaga
     2641 ggacatcctc ctgccgactg ggaaagcctc acccaagaga cagaagaaaa ccattgatgg
     2701 tgttcacaat gcccgccgaa cgtattcgtg ttgaccagcg ggcacccttt ctcactcccc
     2761 gaccagaatc acttcaaccc cagcctttcg cagtccttcc agactatccg taggaatgcc
     2821 ttcatcaaca atgatcatgt cgatacgttg agtatcaatg atcttatgta aactggaacg
     2881 attgaactta ctggaatcgg tgactacgat gatccgttcc gcaacttcgc acatccggcg
     2941 gtttaaacgg gcttcatctt cattatgcgt gctgacgccg cgctccagat cgatcgcatc
     3001 tacaccaaga aacagcatat cgaagtggta attttgcagc gattgttcag cctgatcgcc
     3061 gtaaaaagat tgcgactgac ggcgcaaatg cccgccggtc atcagcagct caacgccttc
     3121 cgcttccagc aaagcattag ccacgttcat accgttggtc atcgcaatta cgtcagtgtg
     3181 cttgcgcatc agacgagcaa tctcaaaagt ggtagtcccg gaatcgagga tcacccgatg
     3241 acctggctga atcaactcaa cggcagcttt cgcaacgctg cgtttcatcg cggtgttcag
     3301 tgcgctttta tcttccactg atggctcgac tgacggcgtc gtgctatcgc agatcaacgc
     3361 gccaccatag gcacgcacag cgatcccctg cttttccaga aacgccagat cgttgcggat
     3421 cgtcacggta gatacgccat acaatgccga cagatcgtta acctgcacac tcccttgctg
     3481 tcgcagacgc tgaatgatct gttctcgtcg ctcgctggtg cctgtcactc gcttctcacc
     3541 tgaagcgtcg gtattactca tagtaagtcc tttcgtaaaa ctttcgtttc atttcgtttt
     3601 gcctattaac gcctttctat taagcaaatg caagcccacc ttgcccattg acgcaagcta
     3661 ctctcgtttc agtgactttc attatgtttc ttttgtgaat cagatcagaa aactattatc
     3721 tttcgtttta tttttatctc accatgacgc agtatcaact gaaacaaaac gaaagattaa
     3781 tatcgcagta atctgaactg gagaggaaag tgaaacatct aacagaaatg gtgagacagc
     3841 acaaagcggg caaaacaaat gcaatttatg ccgtttgttc cgcacatccg ctggtgctgg
     3901 aagctgcaat ccgctacgcc agtgcaaacc aaacgccgtt actgattgaa gcaacctcca
     3961 atcaggtaga ccagttcggc ggttataccg gaatgacgcc cgccgatttt cgcggctttg
     4021 tttgtcagct cgccgactcg ttgaatttcc cgcaggatgc gttgattctg ggtggtgacc
     4081 atctggggcc aaaccgctgg caaaacctgc cagccgctca ggcaatggcc aatgccgatg
     4141 atttgattaa aagctacgtt gcggcaggat tcaaaaaaat ccaccttgat tgcagcatgt
     4201 cctgtcagga cgatccaatt cccttaactg atgacatcgt ggctgaacgc gccgcccgtc
     4261 tggcgaaagt ggcggaagaa acctgtcttg aacactttgg cgaagccgat ctggagtatg
     4321 tcattggtac cgaagtgccg gtacctggcg gcgcgcatga aaccttaagc gagctggcgg
     4381 tcaccacgcc ggatgccgcc cgcgccacgc tggaagccca tcgtcacgcc tttgaaaagc
     4441 aaggtttgaa tgccatctgg ccacgcatca ttgccctggt ggttcaaccc ggcgtcgaat
     4501 tcgatcacac caacgttatt gattatcagc ccgccaaagc gagcgcctta agccagatgg
     4561 tcgaaaacta cgaaacgctg attttcgaag cgcactctac cgattatcaa acgccgcaat
     4621 cgctgcgcca gctggtgatt gaccactttg ccattctgaa agttggccca gcgctgacct
     4681 tcgccctgcg tgaagctctg ttctctctgg cggcgattga agaagaactg gtgccagcga
     4741 aagcctgttc tggtctgcgt caggtgctgg aagacgtgat gctcgaccgc ccggaatact
     4801 ggcaaagcca ctaccacggt gacggcaacg cgcgtcgtct ggcgcgtggt tatagctact
     4861 cggatcgcgt gcgctattac tggccggaca gccagattga tgacgctttc gctcatctgg
     4921 tacgtaatct ggcggattca ccaattccgc tgccgctgat cagccagtat ctgccgctgc
     4981 agtacgtgaa agttcgctcc ggcgagctgc agccaacgcc acgggaactc attatcaacc
     5041 atattcagga catcctggcg cagtaccaca cagcctgtga aggccaataa gcaaaacaaa
     5101 gaggaacacg ctatgccaaa tattgtttta agccggattg atgaacgctt gattcacggt
     5161 caggtcggcg ttcaatgggt cggatttgcg ggggcaaatc tggtgctggt agccaacgat
     5221 gaggttgccg aagatccggt acaacaaaac ctgatggaaa tggtactggc agaagggatc
     5281 gccgtacgtt tctggacgct gcaaaaagtt atcgacaaca ttcatcgcgc cgccgatcga
     5341 cagaaaatcc tgctggtttg taaaacaccc gccgatttcc tgacgctggt gaaaggtggc
     5401 gttccggtga atcgcattaa cgttggcaat atgcactacg ccaatggcaa acaacaaatc
     5461 gccaaaacgg tttctgtgga tgcgggcgat atcgcagcat ttaacgacct gaaagccgct
     5521 ggggtggaat gcttcgttca gggcgtcccg acagagcctg ctgtggacct ctttaaatta
     5581 ctttgaggga ttcatcatgg aaatcagcct gttgcaggca tttgcgttgg gcattatcgc
     5641 ctttatcgct ggcctggata tgtttaacgg cctaacacat atgcaccgcc cggtggtcct
     5701 cggcccgttg gtcgggctgg tacttggcga tctgcatacc ggaattttaa ccggcggtac
     5761 gctggaactg gtgtggatgg ggctggcccc gctggcgggc gcacagccgc ctaacgtgat
     5821 tatcggtact atcgtcggca cggcgtttgc cattactact ggcgtgaaac ccgatgtcgc
     5881 agtcggtgtc gccgtacctt tcgctgtcgc agtacagatg gggattacct tcctgttctc
     5941 ggtgatgtcc ggcgtgatgt ctcgctgcga ccggatggcg gagaacgccg atactcgcgg
     6001 cattgaacgc gtgaactatc tggcactgct ggcactcggc actttctatt ttctctgcgc
     6061 tttcctgcct atctacttcg gcgcggaaca tgcaaaaacc atcattgatg tcctgccgca
     6121 acgattaatc gacggcctcg gcgtcgcggg cggcatcatg ccagcaatcg gttttgccgt
     6181 gctgctgaaa atcatgatga aaaacgtcta catcccctac ttcatcctgg gctttgttgc
     6241 cgcagcctgg ctcaagttac cggtgctggc tattgctgcc gctgcgctgg cgatggcgct
     6301 gatcgacctg ctgcgtaaat ctcctgaacc gactcaacct gcggcacaga aagaggaatt
     6361 tgaagatggc atctaatcaa accaccctgc cgaacgtcag tgaaaacgaa gaaacattgc
     6421 tgactggcgt caatgaaaac gtgtatgaag atcagagcat tggcgcggag ctgacgaaaa
     6481 aagatatcaa ccgcgtcgcc tggcgttcca tgctgttaca agcgtctttt aactacgaac
     6541 gtatgcaggc ttctggctgg ttgtacggtc tgctgcctgc tctgaaaaag atccacacta
     6601 ataaacgcga cctggcgcgc gccatgaagg ggcatatggg tttcttcaat acccatccgt
     6661 ttctggtgac atttgttatc ggcattatcc ttgcgatgga gcgttctaag caggacgtta
     6721 acagtattca gagcaccaaa attgccgtcg gtgcgccgct cggcgggatt ggcgatgcaa
     6781 tgttctggct aacgctactg ccgatttgtg gcgggattgg agccagtctg gcattgcaag
     6841 gctccattct tggcgcagtc gtctttattg tgctgttcaa cgtggtacac ctggggctgc
     6901 gttttggtct ggcgcattat gcttaccgca tgggcgtggc ggcgattcca ctcattaaag
     6961 cgaataccaa aaaagtcggc catgctgcgt caatcgttgg gatgacggta atcggcgcgc
     7021 tggtggcaac ctatgttcgt ttgagcacca cgttggaaat cacagcgggc gacgcagtgg
     7081 ttaagttaca ggctgatgtt atcgacaaac tgatgcctgc cttcttaccg ttggtctaca
     7141 ccctgaccat gttttggttg gtacgccgcg gctggagtcc gctgcgtctg attgcagtta
     7201 ccgtggttct cggcatcgtc ggtaaattct gccatttcct ttaaatacaa tgaggtatta
     7261 gatgttaagt attattttga cagggcatgg cggatttgcc agcggcatgg aaaaagcaat
     7321 gaagcaaatc ctcggcgagc aatcgcagtt tatcgccatc gattttccgg aaacctcaag
     7381 caccgcgctg cttacatcgc agcttgaaga ggccatcgcc caactggatt gtgaagacgg
     7441 cattgttttt ctgacggatt tgctgggcgg cacgccgttt cgcgtagctt caacgctggc
     7501 gatgcaaaaa ccgggctgtg aagtgatcac cggcaccaat ttgcaactgc tactggagat
     7561 ggtgctggag cgcgaagggt taagcggtga agagttccgt gtgcaggcgc tggagtgtgg
     7621 acaccgtggg ctgaccagtc tggtagacga gttaggccgc tgccatgaag aatgtccggt
     7681 cgaggaagga atatgacaca cgttctgcgc gccagaaggc tgctgactga agagggatgg
     7741 ctcgatgacc atcagttgtg tattgctgac ggtgtaatcg cagcaatcga accgattcca
     7801 gtgggcgtga ctgaacgcga tgcggaactg ctctgccccg cttacatcga cacccatgta
     7861 cacggtggtg cgggcgttga tgtaatggat gacgcgccgg atgtactcga caagctggca
     7921 atgcacaagg cacgcgaagg tgtcggcagt tggttgccga ctaccgtaac ctcgccgctt
     7981 aacaccattc atgcggcgct gaaacgtatt gctcaacgtt gccaacgcgg cggacctggt
     8041 gcgcaagtgc tggggagtta tctcgaagga ccgtacttca cgccgcagaa taaaggcgcg
     8101 catccgccgg agctgttccg cgagctggaa attgccgagc tggatcagtt gattgccgtt
     8161 tctcagcaca ccttacgtgt ggtagcgctg gcaccggaaa aagaaggggc attgcaggcc
     8221 atccgccatc ttaaacagca aaacgtaagg gtgatgctgg ggcatagcgc ggcgacctgg
     8281 caacaaaccc gcgccgcatt tgatgctggt gccgacggcc tggtgcattg ctataacggg
     8341 atgacaggtt tacatcaccg cgaaccggga atggttggcg cgggattaac ggacaagcgc
     8401 gcctggctgg aactgatagc cgatggtcat catgtgcatc cggcggcgat gtcgctgtgt
     8461 tgttgctgtg cgaaagagag aatcgtgctg atcaccgacg cgatgcaggc agccgggatg
     8521 ccggatggtc gctatacgtt atgtggtgaa aaagtgcaga tgcacggtgg cgttgtccgt
     8581 accgcgtctg gtgggctggc gggcagtacg ctgtctgttg atgcggcagt gcgcaacatg
     8641 gtcgagttga cgggcgtaac gcctgcggaa gccattcata tggcatcgct gcatccggcg
     8701 cgaatgctgg gtgttgatgg tgttctggga tcgcttaaac cgggcaaacg cgccagcatc
     8761 gttgcgctgg atagcgggct gcatgtgcaa caaatctgga ttcagagtca attagcttcg
     8821 ttttgatagt ttgctccttt attgggcctt cacttccccc gtaaggcctt tctttttctt
     8881 tcgttttgat ctgtgcagcg gtgtcggatg cgacgctaac gcgtcttatg cgacctacgg
     8941 tacgccgcat ccagcaatcg gtgaacatag cgacgtcatt acgcaattct ttaatcttcc
     9001 cttgtctttt ctttcaatga tcacagattt cgttttattt cctttttctc cattgaactt
     9061 tcggtttctt ttctatagat tttaatcaac gaaagacatc accaagtgaa atgaaacgaa
     9121 aggcaagtga aagcgacaac gcccgacgtc aagttcatca gactaaggat tgagttatgc
     9181 caaaaaatta cacccctgct gccgccgcaa ccggtacatg gactgaagaa gagatccgcc
     9241 atcagcctcg cgcatggatc cgttcactca ccaacatcga cgcgctacgt tccgcgctca
     9301 ataacttcct tgaaccgtta ctgcgcaaag agaatctgcg gatcatcctg accggagccg
     9361 gaacctcggc atttatcggt gacatcatcg cgccgtggct cgccagccat accggtaaaa
     9421 acttcagcgc cgtaccgacc accgatctgg tcactaatcc gatggactac ctgaacccag
     9481 cccatccgct gctgttgatc tccttcggtc gatccggcaa cagcccggaa agcgtcgcag
     9541 ccgtggaact ggcaaatcaa tttgtaccgg aatgctatca cctgccgatc acctgcaacg
     9601 aagcgggcgc tctttaccaa aacgcgatca acagcgataa cgcgtttgcc ctgctgatgc
     9661 ccgcagaaac gcacgatcgc ggctttgcga tgaccagcag cattaccacc atgatggcca
     9721 gctgcctcgc ggttttcgca cctgagacga tcaacagcca aaccttccgc gacgtggcgg
     9781 atcgttgcca ggcgatcctg acctcactgg gcgatttcag cgaaggtgtg tttggttacg
     9841 caccgtggaa acggatcgtt tatctcggta gcggtggctt acagggcgca gcacgcgagt
     9901 cggcgctgaa agtgctggaa ctgacggcgg gtaaactggc ggccttttat gattctccaa
     9961 ccggattccg tcatggacca aaatcgctgg tcgatgacga aacgctggtg gtggtatttg
    10021 tctccagcca cccttacacc cgtcagtatg atcttgatct gctggctgaa cttcgccgtg
    10081 acaaccaggc aatgcgtgta atcgccatcg ccgcggaaag cagcgacatc gtcgctgccg
    10141 gtccacatat catcctgcca ccgtcacgtc actttatcga cgttgagcag gcattttgct
    10201 tcctgatgta cgcccagacg tttgcactga tgcagtcgct gcacatgggc aatacgccgg
    10261 ataccccatc agccagtggc accgttaacc gcgtggtgca aggcgtaatc attcatccgt
    10321 ggcaggcata agaggatcgc attatgagca ttatctccac taaatatctg ttacaggacg
    10381 cccaggccaa tggctacgcg gtgcctgctt ttaacattca taacgccgag acgatccaag
    10441 cgatcctcga agtgtgcagt gaaatgcgat cgccggtgat cctcgccgga acgccgggga
    10501 cctttaaaca catcgcgctg gaagagatct acgccctgtg tagcgcctat tccacaacct
    10561 acaacatgcc actggcgctg catctcgacc accacgaatc gctggatgat attcgccgta
    10621 aagtccacgc aggcgtgcgc agtgcgatga tcgacggcag ccacttcccg tttgccgaga
    10681 acgtaaagct ggtgaaatcg gttgtcgact tctgccactc ccaggattgc agcgtggaag
    10741 cagaactggg ccgcctgggc ggtgttgaag atgacatgag cgttgacgcc gaaagtgcat
    10801 tcctgaccga tccacaagaa gctaaacgct ttgtcgaact gactggcgtc gacagcctgg
    10861 cggtagcgat tggtacggcg cacggcttat acagcaaaac gccgaagatt gatttccagc
    10921 ggctggcgga aattcgtgaa gtggtggatg ttcctctggt gctgcatggt gccagcgatg
    10981 ttccggatga atttgtccgt cgcactattg aacttggcgt cacaaaagtg aacgttgcca
    11041 cagaattaaa aatagccttc gctggcgcgg ttaaagcctg gtttgcggaa aatccgcagg
    11101 gtaatgatcc tcgttattat atgcgcgtcg gaatggatgc gatgaaagaa gttgtcagaa
    11161 ataaaattaa tgtctgtggt tcagcgaatc gaatttcagc ataatcatta gttttttatg
    11221 atttatccca atgtacttcc cgattaatat cggggagtgc cttaatggaa aaggagataa
    11281 ctaaacctta ataa
//
LOCUS       DBANUU010000139        11268 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000139 DBANUU010000000
VERSION     DBANUU010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.140"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..682
                     /gene="gspC"
                     /locus_tag="ACIE68_003811"
     CDS             <1..682
                     /gene="gspC"
                     /locus_tag="ACIE68_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFX5910059.1"
                     /translation="GQIINQPTNAFQSDFSLAALWRNENHAGVKDANPVAVNQETPKL
                     SIALNGIVLTSNDETSFVLINEGSEQKRYSLNEALESAPGTFIRKINKTSVVFETHGH
                     YEKVTLHPGLPDIIKQPDSESQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFTTSL
                     LQPGDIALRINNLSLTHPDEVSQALSLLLTQQSAQFTIRRNGVPRLINVSVGELTGMN
                     GLRHERTQ"
     gene            666..2618
                     /gene="gspD"
                     /locus_tag="ACIE68_003812"
     CDS             666..2618
                     /gene="gspD"
                     /locus_tag="ACIE68_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFX5910060.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNARKPSSSGAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPIFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYHSLSKEKYTRYRQEQQQRIDGKSK
                     ALVGSEDLPVLDENTFNSHAPAPSSR"
     gene            2628..4109
                     /gene="gspE"
                     /locus_tag="ACIE68_003813"
     CDS             2628..4109
                     /gene="gspE"
                     /locus_tag="ACIE68_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFX5910061.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQKVISGDTSWDEVMRVASATLESEA"
     gene            4106..5302
                     /gene="gspF"
                     /locus_tag="ACIE68_003814"
     CDS             4106..5302
                     /gene="gspF"
                     /locus_tag="ACIE68_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFX5910062.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLGDVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIGVVIILLTAVVPKITEQFMHMKQQLPLSTRILLGLSDTLQRTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5312..5749
                     /gene="gspG"
                     /locus_tag="ACIE68_003815"
     CDS             5312..5749
                     /gene="gspG"
                     /locus_tag="ACIE68_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFX5910063.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     VVSDIVALENALDMYKLDNHHYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5757..6266
                     /gene="gspH"
                     /locus_tag="ACIE68_003816"
     CDS             5757..6266
                     /gene="gspH"
                     /locus_tag="ACIE68_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFX5910064.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDVTNTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESQND
                     WDEELSIHLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVCSGSWPLD
                     QTLARDTRP"
     gene            6263..6640
                     /gene="gspI"
                     /locus_tag="ACIE68_003817"
     CDS             6263..6640
                     /gene="gspI"
                     /locus_tag="ACIE68_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFX5910065.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRNAIERMRNET
                     LALWIADNQLQSQDSFGEENTSSSGKELINGEEWNWRSDIHSSKDGTLLERTITVTLP
                     SGQTTSLTRYQSIDNKSGQAQDD"
     gene            6633..7220
                     /gene="gspJ"
                     /locus_tag="ACIE68_003818"
     CDS             6633..7220
                     /gene="gspJ"
                     /locus_tag="ACIE68_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFX5910066.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     QTQQYGGLQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7213..8196
                     /gene="gspK"
                     /locus_tag="ACIE68_003819"
                     /pseudo
     CDS             7213..8196
                     /gene="gspK"
                     /locus_tag="ACIE68_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
     gene            8211..9374
                     /gene="gspL"
                     /locus_tag="ACIE68_003820"
     CDS             8211..9374
                     /gene="gspL"
                     /locus_tag="ACIE68_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFX5910067.1"
                     /translation="MPESLMVIRSSSTLRKHWEWMTFSADSVSSVHTLTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDNTQDWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQKSWLIR
                     SAAHAFNELDEHWLQHLAAQFPPENMLCYGVVPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGIFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRSIALWHTLKIEDQL
                     QQQQQETWQRYFPQIKRTHNFHFYFKQQLAQQYPEAVPLLYHLQTLLLEHPELQLMEA
                     NYSQKQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTVRNSGK"
     gene            9371..9832
                     /gene="gspM"
                     /locus_tag="ACIE68_003821"
     CDS             9371..9832
                     /gene="gspM"
                     /locus_tag="ACIE68_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFX5910068.1"
                     /translation="MIKSWWAEKSTSEKQIVAALAVLSLGVFCWLGVIKPIDTYIAEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLAITLENGPDNTL
                     TIHPVTAPLENVSRWLTTAQVTYGIVIEDLQFTLAGNEEITLRHLSFREQQ"
     gene            9832..10502
                     /gene="gspO"
                     /locus_tag="ACIE68_003822"
                     /pseudo
     CDS             9832..10502
                     /gene="gspO"
                     /locus_tag="ACIE68_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
     gene            complement(10531..11007)
                     /gene="bfr"
                     /locus_tag="ACIE68_003823"
     CDS             complement(10531..11007)
                     /gene="bfr"
                     /locus_tag="ACIE68_003823"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFX5910069.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(11079..>11268)
                     /gene="bfd"
                     /locus_tag="ACIE68_003824"
     CDS             complement(11079..>11268)
                     /gene="bfd"
                     /locus_tag="ACIE68_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFX5910070.1"
                     /translation="VCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVRAA
                     REVMEDELMQLPEFKESA"
BASE COUNT         2934 a         2901 c         2713 g         2720 t
ORIGIN      
        1 tggacagatc attaatcaac caacaaacgc atttcagtct gatttcagcc ttgccgcttt
       61 atggcgtaac gaaaatcatg ctggggtaaa agatgcaaat cccgtggcag ttaatcagga
      121 aacacctaag ctctctatag cgcttaatgg cattgtactc acatcgaatg atgagacttc
      181 ttttgtctta attaatgagg ggagcgaaca aaagcgatac tccctcaacg aagcccttga
      241 gtcagcgccg ggaacgttta ttagaaaaat aaataaaacc agcgttgtgt ttgaaactca
      301 cgggcattac gaaaaagtaa ctctccatcc agggctacct gacatcatca agcagcctga
      361 ttcagaaagt caaaatgtac tcgctgatta tattatcgcc acgcctatac gtgatggaga
      421 acagatatac gggcttcgat tgaaccctcg taagggcctc aatgcattta ccaccagctt
      481 gctacaacca ggggatatcg ccctcaggat caataatctt tcgctgaccc accctgatga
      541 ggtttcacag gcattaagtt tactgctaac tcaacaaagt gctcagttta caattcgtcg
      601 caacggcgta ccccgcttga taaatgtttc cgtcggggaa cttacaggaa tgaatggact
      661 gcgtcatgaa aggactcaat aaaatcacct gctgcttgct ggcagcacta ctcatgcctt
      721 gtgcaggaca cgctgagaac gaacaatacg gcgcgaactt caataacgcc gatatccgcc
      781 agttcgtgga aatagtgggt cagcatcttg gcaaaacgat cctgatcgac ccttcggtac
      841 agggaaccat ttccgtacgc agtaatgata cgtttagcca acaggagtac taccagttct
      901 ttttaagtat tcttgatctt tacggttatt ccgtgatcac gctggacaat ggttttctga
      961 aagtggttcg ctcagctaat gtaaaaacat cgccagggat gattgctgac agttctcgtc
     1021 caggcgtagg tgatgagttg gtcacccgaa ttgtaccgct tgagaacgtt cctgctcgtg
     1081 acctggcccc cctgctccgc cagatgatgg atgcgggtag cgtcggtaat gttgtgcatt
     1141 atgaaccctc caacgttctt attctgaccg gtcgtgcctc caccattaat aaactgattg
     1201 aagtcataaa gcgcgttgat gtcatcggca cagagaagca gcaaattatt catctggaat
     1261 atgcgtcagc ggaagatctc gccgagattc ttaatcaatt aatcagcgaa agccacggta
     1321 aaagccagat gccagccctc ctctccgcga agattgtggc ggataagcga accaactctc
     1381 ttatcatcag tggaccggaa aaagcacgcc agcgcatcac ttcattactg aaaagccttg
     1441 atgtcgaaga gagcgaggaa ggaaataccc gggtttatta cctgaaatat gctaaagcca
     1501 cgaatctggt ggaagtgcta accggtgttt ccgaaaagct gaaagatgaa aaagggaatg
     1561 cgcgtaagcc ctcctcttct ggcgcgatgg ataacgtcgc cattaccgcc gatgaacaga
     1621 ctaactctct ggtcattacc gctgaccagt ccgtccagga aaaactcgcc acggtaattg
     1681 cgcgtctgga cattcgccgt gcacaggtgc tggttgaggc aatcatcgtt gaagttcagg
     1741 atggaaatgg actaaacctc ggcgtgcaat gggcgaataa aaacgttggc gcacagcaat
     1801 ttaccaatac cggattaccg atttttaacg ctgcgcaagg tgtggctgat tataaaaaga
     1861 atggtgggat caccagcgcg aatcctgcct gggatatgtt tagcgcctac aatggcatgg
     1921 ccgcaggctt cttcaatggc gactggggag tactgcttac cgcgctggcc agtaacaata
     1981 aaaatgacat cctcgccacc ccaagcatcg taacgctgga taataaactc gcgtccttca
     2041 acgtggggca ggatgtgccg gtgctatccg ggtcacagac cacttcaggg gataacgtct
     2101 ttaataccgt cgaacgcaaa acggtgggga caaaactcaa agttactccg caggtcaatg
     2161 aaggcgacgc ggtgttgctc gaaatagagc aggaagtctc cagcgttgac tcttcctcta
     2221 actcgacgct cggcccgacg tttaataccc gtactattca aaacgccgtg ctggtcaaaa
     2281 ccggtgaaac ggtggtcctg ggcggattgc tggatgattt ttctaaagag caagtgtcaa
     2341 aggttcctct gcttggcgat attcctttag tggggcaact cttccgctat acctccaccg
     2401 agcgcgctaa acgcaacctg atggtattta tccgtccgac gattatccgt gacgatgatg
     2461 tttatcactc actgtcaaaa gagaaataca cccgttaccg tcaggagcaa caacagcgga
     2521 tcgacgggaa atcaaaagcg ctggttggct cggaagattt gccggtgctg gatgaaaaca
     2581 cgttcaacag tcacgcccct gcgccatcgt cacggtgagg cattcatatg agaattcact
     2641 caccgtaccc cgccagttgg gcgctggcac aacgaattgg ttatctctat tcagagggcg
     2701 agattattta tctcgccgat acgccattcg agcggttact cgatattcaa cgtcaggttg
     2761 gccagtgcca gaccatgacc agcttgtcac aggctgattt tgaagctcgg ctggaagcgg
     2821 tattccatca gaataccggt gagtcgcaac agattgcgca ggatatcgat caatccgtcg
     2881 atcttctctc gctttcggaa gagatgcccg caaatgaaga tctcctgaat gaagattcag
     2941 cggcaccggt tatccgcttg atcaatgcga ttttgagtga ggccatcaaa gaaaccgcct
     3001 ctgatatcca cattgaaacc tatgaaaaaa caatgtcgat ccgttttcgc atcgacggcg
     3061 ttttgcggac aattttacag ccaaacaaaa aactggcggc actgcttatc tcccgaatta
     3121 aggtcatggc tcgtcttgat atcgccgaaa aacgtattcc acaggatgga agaattagtt
     3181 tgcgtatcgg gcgacgtaac atagatgtcc gcgtatccac actgccgtcc atctatggtg
     3241 aacgcgccgt actccgcctg ctggataaaa acagcctcca gctttcattg aacaacctgg
     3301 ggatgacggc agcggataag caggatttag aaaatctcat tcagcttccg cacggtatta
     3361 tcctggtgac agggccgaca ggctccggta aaagcaccac gctctacgcc atcctttcgg
     3421 cgctgaatac tcccggccgc aatattctga cggtagaaga tcccgtggaa tatgagctgg
     3481 aaggcattgg gcaaacgcag gtgaataccc gtgtggatat gtctttcgct cgcggcctgc
     3541 gcgccatact tcgccaggac ccggatgtcg tcatggtggg ggaaattcgt gatacagaaa
     3601 ccgcgcagat tgcggttcag gcctcgctca ccggccatct ggtactctca acactccaca
     3661 ctaacagtgc atcaggcgca gtgacccggc tccgcgacat gggcgtcgaa tcattcctgc
     3721 tttcgtcttc cctggcaggg attatcgcgc aacgtctggt tcgtcgcctg tgtccgcaat
     3781 gccgacaatt cacgcccgta tcaccccaac aagcgcagat gtttaaatat catcagctcg
     3841 cggtgacaac aattggcact cccgtaggct gccctcattg ccatcaatcc ggctatcagg
     3901 ggcgcatggc gatccacgaa atgatggtgg tgacgccgga attacgggcc gctattcatg
     3961 aaaatgtgga tgaacaagca ctggagcgac tagtccggca acaacacaat gccttaatca
     4021 aaaatggcct gcaaaaagtg ataagcggtg acacctcctg ggatgaggtt atgcgcgtcg
     4081 ccagtgccac gctggagagc gaagcatgaa ttatcgctat cgcgccatga cccaggatgg
     4141 tcaaaaattg caagggatca ttgatgctaa cgatgaacgt caggcacgac tgcggctgcg
     4201 tgaagaaggg cttttcctgc tggatattcg cccccaaaaa agttcgggag taaaaacacg
     4261 tcgcccgagg atcagccata gtgaactgac gcttttcacc cggcagttgg caaccttaag
     4321 cgcagcggca ttacccctgg aagagagcct tgccgtaatc ggtcaacaaa gcagtaataa
     4381 acgactgggt gacgtgttaa atcaggtacg cagcgccatc cttgaagggc atcccctttc
     4441 cgatgcatta cagcattttc ccacgctttt cgattcgctc tatcgtaccc tggtaaaagc
     4501 gggcgaaaag agcgggctgc tggccccggt gttggaaaag ctggctgatt acaatgaaaa
     4561 ccggcagaaa atccgcagca agctcattca gtcactgatc tacccctgta tgctcactac
     4621 ggtggcgatt ggggtcgtga ttattctcct cactgctgtc gtgcccaaaa ttaccgaaca
     4681 gttcatgcat atgaagcagc aactgccgct gagtacacgc attcttttag gtctgagcga
     4741 cacgttgcaa cgtaccggcc cgacattatt agcgacagtg tttattgtcg ctgtaggttt
     4801 ctggctctgg ttaaaacgcg gcaataaccg ccaccgtttt catgccatgt tgctgcgcgt
     4861 tgcgctcatc ggcccgctga tttgcgccat taacagcgca cgctatctcc gcactttaag
     4921 tattttgcaa tccagcggcg tccctctgct ggatgggatg aatttgtcca ccgaaagcct
     4981 caacaacctt gaaattcgcc agcgtctggc aaatgcggca gagaacgttc gccagggtaa
     5041 cagcattcat ctttcgctgg aacaaaccgc aattttcccg ccgatgatgc tctacatggt
     5101 ggcctctggc gaaaaaagcg ggcagctcgg cacattaatg gtcagagccg cagataacca
     5161 ggagacactc caacaaaatc ggatcgcctt aacgctctcc atcttcgagc cagcactcat
     5221 tattacgatg gcactgatcg tcctgtttat tgtcgtgtcg gtactccaac ctcttcttca
     5281 acttaactca atgattaatt aaggaaagca tatgcgcgca acggataagc aacgcggttt
     5341 tacattactg gaaattatgg tggtgatcgt cattatcggc gtacttgcca gcctggtggt
     5401 tcctaaccta atgggcaata aagaaaaggc ggataagcaa aaagtcgtca gcgatattgt
     5461 ggcgctggaa aacgcccttg atatgtacaa actcgacaat catcactatc caaccacaaa
     5521 tcaggggctt gaatccttag tagaagcgcc gacactgcca ccgctggccg caaactataa
     5581 caaggaaggt tatatcaagc gtctgcctgc cgatccctgg ggcaatgatt atgtcctcgt
     5641 taatcctggt gaacatggtg catacgatct gctttcagca gggcccgatg gtgaaatggg
     5701 aaccgaggac gacatcacca actggggttt gagcaagaag aaaaagtaag tcagcgatga
     5761 atcagcaacg cgggtttacc ctgctggaga tgatgctggt gctggcgctg gtcgctatca
     5821 cggcaagcgt ggtgctcttc acatatggcc gggaggatgt caccaacact cgtgcgcgcg
     5881 aaacagcggc gcggtttacc gctgcacttg aattagccat cgaccgcgcc acgctcagtg
     5941 gtcagcctgt cggtatccat ttctctgatt cagcatggcg cattatggta ccgggcaaaa
     6001 caccatcagc ctggcgctgg gttccattac aagaagatgc cgcagacgag agccaaaatg
     6061 actgggatga agaactctca attcatcttc agccatttaa acctgatgac agcaaccagc
     6121 cacaggttgt gattctggcc gacggacaaa tcacgccctt ctctttgttg atggctaacg
     6181 ccgggaccgg tgagccgctc ctgacgttag tctgttcagg ctcatggccc ctggatcaaa
     6241 cgctcgccag agatacccgc ccatgaacaa acaatcaggg atgacactgc ttgaagtctt
     6301 actggcgatg agtattttca ccgctgttgc tctgacttta atgagcagta tgcaagggca
     6361 acgaaatgcc atcgagcgaa tgcgtaacga gacgctggca ttgtggatag cggataacca
     6421 gcttcagtca caagattcat tcggcgagga aaacacgtcc agttctggca aagagttaat
     6481 aaatggcgaa gagtggaact ggcgcagcga tatccactca agcaaagacg gcacgcttct
     6541 ggaacgcacc ataacggtga cattacccag cggtcagaca acctcgctta cgcgttatca
     6601 aagcatcgat aataaatccg ggcaggcaca agatgattaa tcgccagcag ggtttcacat
     6661 tgctggaggt catggcggcg ctggcgattt tctcaatgct tagcgtgctg gcgtttatga
     6721 ttttttcgca agcttccgag ttgcatcaac gctcacagaa agaaatccag cagttcaatc
     6781 agctacagcg caccatcacc attcttgata acgatctcct gcaactggtc gcccggagga
     6841 atcggagtac ggacaaaatc atggtgttgg gtgaagaagc tatttttacc acgcaaagtc
     6901 gcgatccgct ggctcctctc agtgaagcac aaaccttact cactgttcac tggtatctgc
     6961 gaaatcatac gctctaccgt gctgttcgta cttctgtgga tggcaggaag gatcaacccg
     7021 cccaggcgat gctggaacat gtcgagagct ttcttctgga aagtaacagc ggggaaagcc
     7081 aggaacttcc tctctcagtc acgttacatc tgcagactca gcaatatggg gggttacagc
     7141 gccgttttgc attacctgag cagttagcga gagaagagtc gccagctcag acgcaggccg
     7201 ggaataataa ccatgaataa tgaacaacgc ggcgtcgcgc tgctcattgt actcatgtta
     7261 ctggcgctca tggctgctct ggcagcagac atgacgctca gttttcacag ccagcttcag
     7321 cgaacccgcc aggtaaacca tcatctgcaa cggcaatatg atattgaact ggcggagaaa
     7381 cttgcgcttg ccagcctcac ccaggacgtt aaagataacg acagacagac cacgttgcag
     7441 cagtactggg cacagccaca acagttgcag ctggaagatg gcaatacagt gaagtggcag
     7501 ttacgcgatg cccagcactg ttttaatctc aatgcgttag caaaaatctc tgacgacccg
     7561 ctggcttccc ctgatttccc cgcccaggta ttcagtgcgc tgctgattaa cgccggtatc
     7621 gatcgcggaa acaccgatga gatcgtgcaa tcaatcgctg actatattga tgtcgatgac
     7681 tcaccacgct ttcacggcgc agaagatagc ttttaccaaa gccagacgcc gccccggcat
     7741 agtgccaatc agatgctttt tctgactggc gaattacgtc agataaaagg cataaccgaa
     7801 aacatctatc agcggcttat cccgtatgtc tgcgttctcc cgaccacgga acttagcatc
     7861 aatcttaata tgctaacgga gaacgatatt ccgctcttca gagcgttatt cctcaataac
     7921 atcacggatg ctgacgctcg ggtcttattg caaaaaaggc cgagggaagg ctggctaacc
     7981 acggatgcat ttctttactg ggcgcagcag gatttctccg gtgtgaagcc actggttgct
     8041 caggtgaaaa ggcatctctt tccctatagc cgctatttta cgctgagtac ggaaagtatc
     8101 agcgacgaac aatcctaggg atggcaaagc catatttttt tcaaccgtaa acagcagagc
     8161 gcccaaatct atcggcgtac gctgcaactc tactaatcat aaggatcgtg atgcctgaat
     8221 cgttgatggt catacgttct tcctcgacgc tgcgtaagca ttgggaatgg atgacgttct
     8281 ccgcagatag cgtatcttcg gtacatacgc tcacggacga tcttccgctg gagtcgctgg
     8341 ccgatcagcc cggtgccgga aatgttcatc ttttgatccc accagagggc cttttatatc
     8401 gttccctcac gctgcccaac gccaaataca aactcaccgc acaaacatta caatggctgg
     8461 cagaggagac gttacccgac aacacccagg actggcactg gacggttgtc gataaacaaa
     8521 acgaaagtgt cgaggtgata ggcattcagt cggagaaact cagtcgctat cttgagcgtt
     8581 tacatactgc cggactcaac gtgacgcggg ttttgccaga tggctgctat cttccctggg
     8641 aagtagatag ctggactctg gttaaccagc aaaaaagctg gctcattcgc agtgccgcac
     8701 atgccttcaa cgaactggat gaacactggt tacagcatct tgctgcccag tttccgcctg
     8761 agaatatgct ttgttatggt gtagtacctc acggcgttgc cgcggccaat ccacttatcc
     8821 agcatcctga aataccatca ctgagtttgt attctgccga tattgcattc cagcgctatg
     8881 acatgctgca tggtattttt cgtaaacaga aaactgtaag caaatcggga aaatggctcg
     8941 cccgcctggc agtaagctgt ctcgttctcg ccattctttc ttttgttggc agccggagca
     9001 tcgcgctctg gcatacgctt aagattgaag atcaacttca gcaacaacag caagaaacct
     9061 ggcaacgtta tttcccgcag atcaaacgca cccacaactt tcatttttac tttaagcagc
     9121 aactcgctca gcaatatcct gaagctgtac cgttgctcta tcatttacaa acacttctgc
     9181 tggaacaccc tgaactgcag ttaatggagg cgaactatag ccagaagcaa aagtcgctga
     9241 ccttaaaaat gagtgctaag agtgaagcca atatcgatcg tttctgtgag ttaacacagt
     9301 cctggcttcc gatggaaaaa acagaaaaag atccggtcag tggtgtatgg acagtaagga
     9361 actcagggaa atgattaaat catggtgggc agaaaaatcc acatcggaaa aacagatcgt
     9421 tgcggcactg gcggttctct cgctcggcgt tttttgttgg cttggggtga taaaaccgat
     9481 agatacctac attgcagagc atcagtccca cgcgcaaaaa attaagaagg acatcaaatg
     9541 gatgcaggat caagcgagca cccatggttt actcggtcat cctgcgctta cgcagcccat
     9601 caaaaatatc cttctcgagg aagcaaaacg tgagaatttg gctatcacgc tggaaaacgg
     9661 cccggacaat actctgacaa ttcatcctgt cacagctccc ctcgaaaacg tctcccgctg
     9721 gctcactacg gcacaggtaa catacggtat tgttattgaa gaccttcagt ttactcttgc
     9781 cggaaacgaa gagatcaccc tcaggcattt gtccttcagg gagcaacaat aatgacaatg
     9841 ctactgccgc tcttcattct ggttggtttt attgcaggtt attttgttaa tgctatcacc
     9901 tctcgccgtt ggaggataaa acggcgttaa cgtttcgcca ggtattggtt catttcaggc
     9961 aaaaaaaata tgcctggcat gatacagtgc ccctgatact ttgtgttgct gctgctatcg
    10021 cctgcgccct ggcacctttc acgcccatcg tgactggcgc actctttctc tatttctgtt
    10081 tcgtactcac gctcagtgtt attgattttc gcactcagct cctgcccgac aaactcacct
    10141 taccgctgct ctggcttggc ttggtattta atgcgcagta tggattaatt gatttacatg
    10201 atgcggttta cggcgcggta gcgggctatg gggtgctgtg gtgtgtttac tggggcgtct
    10261 ggttagtttg tcacaaagag ggattgggct acggtgattt caagctactg gccgctgcag
    10321 gcgcatggtg tggctggcaa acgttgccaa tgatactgct gattgcctcg ctgggtggca
    10381 ttggttacgc catcgtttca caacttctgc aacgccggac tataaccact atcgcattcg
    10441 gcccgtggct tgcgctcggc agcatgataa acctggggta tctggcctgg atctcttatt
    10501 aaccgggagg gttctccctc ccgacacggc tcaaccttct tcgcggatct gtgcttgcag
    10561 ataattttgc aggcccatct tctgaatcag atcaagttcc gtttccagcc agtcgatatg
    10621 gccttcttca tcacgcaaaa tttctatcat catatcgcgg ctgacgtaat catgaacgct
    10681 atcggcataa ccaattgcct cacgcaaatt cttcgcgcca tccagctcaa gtgccagatc
    10741 agaacgcagc atttcctcaa catcttcacc aatgttcagt ttgcccaggt cctgtaagtt
    10801 tggaagacct tccagaaaaa gaatgcgctc aatataacga tcggcgtgtt tcatctcatc
    10861 aatggattca tgatactcca catcattgag acgtttgaga ccccagtttt taaacattcg
    10921 ggcatggaga aagtactgat tgattgcgac aagctcattt cccaacagtt tgttgagata
    10981 atttataact ttagtatcac ctttcatttt ttgactcctt cgcttccact ctttgaagag
    11041 tagaacggct tacgagtaag tcaaaaaaca cactccgttt atgcggactc cttaaactcc
    11101 ggcagctgca ttaattcatc ctccatcact tcacgcgcgg cccggacaca tttaccgcac
    11161 tgattgccga ccggaataaa tttttttaat tgctggaacg agtgagggga aaactggcgc
    11221 acagcctgac gaattttttt atcgctgata ccattacaaa gacaaacg
//
LOCUS       DBANUU010000140        11035 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000140 DBANUU010000000
VERSION     DBANUU010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.141"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(94..1923)
                     /gene="glmS"
                     /locus_tag="ACIE68_003825"
     CDS             complement(94..1923)
                     /gene="glmS"
                     /locus_tag="ACIE68_003825"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFX5910071.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2085..3455)
                     /gene="glmU"
                     /locus_tag="ACIE68_003826"
     CDS             complement(2085..3455)
                     /gene="glmU"
                     /locus_tag="ACIE68_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFX5910072.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3806..4225)
                     /locus_tag="ACIE68_003827"
     CDS             complement(3806..4225)
                     /locus_tag="ACIE68_003827"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFX5910073.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4246..5628)
                     /gene="atpD"
                     /locus_tag="ACIE68_003828"
     CDS             complement(4246..5628)
                     /gene="atpD"
                     /locus_tag="ACIE68_003828"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFX5910074.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5655..6518)
                     /gene="atpG"
                     /locus_tag="ACIE68_003829"
     CDS             complement(5655..6518)
                     /gene="atpG"
                     /locus_tag="ACIE68_003829"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFX5910075.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6569..8110)
                     /gene="atpA"
                     /locus_tag="ACIE68_003830"
     CDS             complement(6569..8110)
                     /gene="atpA"
                     /locus_tag="ACIE68_003830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFX5910076.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8123..8656)
                     /gene="atpH"
                     /locus_tag="ACIE68_003831"
     CDS             complement(8123..8656)
                     /gene="atpH"
                     /locus_tag="ACIE68_003831"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFX5910077.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8671..9141)
                     /gene="atpF"
                     /locus_tag="ACIE68_003832"
     CDS             complement(8671..9141)
                     /gene="atpF"
                     /locus_tag="ACIE68_003832"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFX5910078.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9203..9442)
                     /gene="atpE"
                     /locus_tag="ACIE68_003833"
     CDS             complement(9203..9442)
                     /gene="atpE"
                     /locus_tag="ACIE68_003833"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFX5910079.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9489..10304)
                     /gene="atpB"
                     /locus_tag="ACIE68_003834"
     CDS             complement(9489..10304)
                     /gene="atpB"
                     /locus_tag="ACIE68_003834"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFX5910080.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10313..10693)
                     /gene="atpI"
                     /locus_tag="ACIE68_003835"
     CDS             complement(10313..10693)
                     /gene="atpI"
                     /locus_tag="ACIE68_003835"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFX5910081.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2635 a         3010 c         2738 g         2652 t
ORIGIN      
        1 gaatatattt atatagaaag atagatgaat ttattgatgt cagttataaa aaagggccac
       61 cggtcggcag ccctttttta tctatgatag tgattactca accgtaaccg attttgccag
      121 gttacgcggc tggtcaacgt cggtgccttt gatcagcgcg acatggtaag ccagcagctg
      181 cagcggaacg gtgtagaaga tcggtgcaat cacctcttcc acatgcggca tctcgatgat
      241 gtgcatgtta tcgctactta caaaacccgc atcctgatcg gcgaagacat acaactgacc
      301 gccacgcgcg cgaacttctt caatgttgga tttcagtttt tccaacagtt cgttgttcgg
      361 tgcaacgacg ataaccggca tatccgcgtc aatcagcgcc agcgggccgt gtttcagctc
      421 gcctgcagcg taggcttcag cgtgaatgta agagatctct ttcagcttca atgcgccttc
      481 cagcgcgatt gggtactgat cgccacggcc caggaacagc gcgtgatgtt tgtcagagaa
      541 atcttctgcc agcgcttcaa tgcgtttgtc ctgagacagc atctgctcaa tacggctcgg
      601 cagcgcctgc agaccatgca caatgtcatg ttcaatggag gcatccagac ctttcaggcg
      661 agacagcttc gccaccagca tcaacagcac agttaactga gtggtgaatg ctttagtgga
      721 tgccacgccg atttccgtac ccgcgttggt catcagcgcc agatcggatt cgcgcaccag
      781 agaagaaccc ggaacgttac agattgccag cgaaccaagg taacccagct ctttcgacag
      841 acgcaggccc gccagggtat ccgcggtttc gccagactgt gacaaggtga tcatcaggct
      901 gttacgacgc acggcagatt tgcgatagcg gaattcagag gcgatttcga cgtcgcacgg
      961 aatacctgcc agcgattcaa accagtaacg ggaaaccata ccggagttat aagaagtacc
     1021 acaggcgagg atctgaatat gctcaacctt cgacagcagt tcgtcggcgt tcggtcccag
     1081 ctcgcttaaa tcaacctgac cgtggctgat gcgcccggta agggtgtttt tgatcgcgtt
     1141 cggctgttcg tagatctctt tctgcatgta gtgacggtaa atacctttat cgcccgcgtc
     1201 atattgcaga ttggattcga tatcctgacg ttttacttcc gcgccagttt tatcgaagat
     1261 gtttaccgaa cggcgagtaa tttccgcaat atcgccctct tcaaggaaga taaagcgacg
     1321 ggtcaccggc aacagcgcca gctggtcaga agcgataaag ttttcgccca tacccaggcc
     1381 aatcaccagc ggactaccag aacgtgccgc cagcagggta tccgggtgac gggagtccat
     1441 gatcactgta ccgtacgcac cacgcagttg cgggatagca cgcagaacag cctcacgcag
     1501 agtaccgccc tgtttcagct cccagttcac cagatgggca attacttcgg tgtctgtttc
     1561 agaaacgaag gtatagccac gcgcttttaa ctcttcgcgc agcggttcat ggttttcgat
     1621 gatgccgtta tgcaccacca caatgtgttc agaaacatgc ggatgcgcat tcacttctga
     1681 aggttcaccg tgggtcgccc agcgagtatg agcaataccc gtaccgccat gcagaggatg
     1741 ttcttccgct gcctgagcca gcatctggac tttaccgagg cgacgcaggc gggtcatatg
     1801 accttccgca tcaacaacgg ccagaccggc agagtcatat ccgcggtatt ccagacgacg
     1861 taaaccttca agaaggattt ctgctacatc acgttgcgcg atcgcgccaa caattccaca
     1921 catagttttt gattccgatt tatatcgttg tcggtcaacc tgtatgcccg ttttttcggg
     1981 cgccccgagc cttgtagaga gtggggttat ttttatagtt actgcttgtg ggagggagat
     2041 tatgttatct cctcatccca gatgaccgga ttacccggcc agaattactt tttctttacc
     2101 ggacgacgcc agccttcttt ctgagtctgc ggcacacggc tgatagctaa tgcattttcg
     2161 ccgacattac gcgtcacagt tgtacccgca gcaatggtcg cgcctttgcc tactgttacc
     2221 ggggccacca gctgagtgtc ggaaccgaca aacacatcgt cgccgataat ggtcttaaat
     2281 ttattcgcac catcgtagtt gcaggtaatg gttcccgcgc cgatgttaac gttatcgcca
     2341 atttccgcat cgcccaggta agtcagatga ccagctttcg agcctttacc cagacgcgct
     2401 tttttcatct caacaaagtt accgacgtgt gcaccttcca gcaactcagc accaggacgc
     2461 agacgggcaa acgggccaat agtacaggcc gctgccagat tcgcgtcttc cacaacggta
     2521 tacggactga tttcgcaatc atcgccaatc acgctgtttt taatcacgca accggtgccg
     2581 attttcacgc gatgaccgag agtcacgttg ccctcgatga taacgttagt atcaatttca
     2641 acatcgcgcc cgtgagtaag cgtaccgcgc agatcaaaac gcgccggatc gcgcagcata
     2701 acgcctgcta acagcagttt ttcagcctgt tcggactggt aaacgcgctc cagacgggag
     2761 agttgcaggc ggttattcac accttctact tcgcttaaac gttgcggatg aacggcgacg
     2821 atttcacgcc cttcctgata cgccagcgca ataatgtcgg tgatgtagta ttcgccctga
     2881 gcattattgt tggtcagctt cgccagccag cgtttcatat ctgcgccgtt agcgatcaga
     2941 atgccggtgt tgatctcctg aatctgacgc tgctcgtcgg tggcatcttt gtgctcaaca
     3001 atgccggtaa ctttgccatt ttcacgggtg atacgtccat aaccggtcgg atcatccagt
     3061 ttcaccgtca gcagaccaat gccaccctgc ggtttagcat cacgcaggcg ctggagtgtt
     3121 tcgacagaga tcagcggcac gtcgccgtag agcattaaaa tgtcttcatc atcggcaaag
     3181 aaaggtgcgg cctgctgcat tgcatgaccc gtacccagct gctctgcctg aagcacccag
     3241 ttaaggttgt cgtctttcag cgcctgttta agcagatcgc cgccgtgacc gtacaccagg
     3301 tgaacgtgcg ctgcgcctaa ttcattcgca gcatcaatga catgctgaac catcgctttc
     3361 ccggcaaggg tatgcagcac tttcggaaga tcggaataca tgcgcgtgcc tttgcctgcg
     3421 gcaaggatca ctacgctcat agcattattc aacatacgcg tcctgactgt aatttgagaa
     3481 cgaatttaaa ccgcttcacc ttgaaaaaac tacatttttt tcatcgtgaa atggacagag
     3541 gataaattgt tcaataacgg attatccccg ctgacgaaag tgccattttc gaccatcgtc
     3601 acgccttttg tctgcagaaa attaaggcag taccttcatt taagcgtcag gtggatgttt
     3661 ttgctcttat ttcgatcaat gaataaacgg gaaaacaggg gtgttttata tcagcaggat
     3721 ctatgtgaac gctcttcagg acggatcacg cgtaaaaaaa agccagcctg tttccagact
     3781 ggcttttgtg cttttcaagc cggtgttaca tcgctttttt ggtcaactcg ataacgcgca
     3841 gctgcgcgat cgctttggcc agttccgcag acgcctgagc gtaatctacg tcgccgtgag
     3901 agctgctaat gtgctcttca gccttacgtt tcgcttccat ggctcgcgct tcgtcgagat
     3961 cctggccgcg aattgcggtg tcggccagaa cggtcacgtt gccaggctgc acttcaagaa
     4021 tgccgccaga cagatagata aactcttcgt gaccgtgctg tttcacgatg cgaatcatac
     4081 caggcttaat ggcggtgagc agcggtgcgt ggccagggta gatccccagt tcaccttcgc
     4141 tacccgttac ctggattttc tcgaccagac cagagaacat ttgttgctct gcgctgacga
     4201 cgtccaggtg gtaagtcatt gccatatcac cctccgatta aggcgttaaa gttttttggc
     4261 tttttccaca gcttcttcga tggaaccgac catgtagaac gcctgctccg gcaggtgatc
     4321 gtattcgcct tccatgatgc ctttaaagcc acggatggtg tctttcaggg agacgtattt
     4381 acccggagaa ccggtgaata cttctgccac gaagaacggc tgggacagga agcgctggat
     4441 cttacgagca cgcgctacca ccagtttgtc ttcttcagac agttcatcca tacccaggat
     4501 ggcgatgatg tctttcagtt cctgataacg ttgcaggatg gactgaacgc cacgcgcggt
     4561 gtcgtagtgt tcctgaccaa ccaccagcgg gtccagctga cggctggtgg agtccagcgg
     4621 gtcaacggcc gggtagatac ccagagacgc gatctgacgg ctcagtacca cggttgcgtc
     4681 aaggtgcgca aaggtggttg ccggagacgg gtcagtcaag tcatccgcag gtacgtatac
     4741 cgcctgtacg gaggtgatag aaccagtttt ggtggaggtg atacgttcct gcagaacgcc
     4801 catctcttcc gccagggtcg gctgataacc taccgctgaa ggcatacggc ccagcagtgc
     4861 ggatacttcc gtaccggcca gggtgtaacg atagatgttg tcaacgaaca gcagaacgtc
     4921 acgaccttcg tcacggaatt tctcagccat ggtcagaccg gtcagagcaa cgcgcagacg
     4981 gtttcccggc ggctcgttca tctggccata caccagggat actttgtcga taacgttgga
     5041 gtcggtcatt tcgtggtaga agtcgttacc ctcacgagta cgttcaccta cgcccgcaaa
     5101 cacagagtaa ccggagtgct cgatcgcgat gttacgaatg agctccatca tgtttacggt
     5161 tttacctaca cccgcaccac cgaacagacc aactttaccg cccttagcga acggacacat
     5221 caggtcgata actttgatac cggtttccag cagttcctga gagtttgaca gctcttcgta
     5281 ggaaggtgct gcgcggtgaa tcgcccaacg ctcttcttca ccgatctcgc ctttcatgtc
     5341 gaccggttca cccagtacgt tcatgatacg gcccagagtc gctttaccta ccgggacttc
     5401 gatcgggtgt tcgaggtctt ttacatccag accgcgacgc agaccgtcgg aggaacccat
     5461 tgcgatggta cgcacgatac cgccgccgag ctgctgctga acttccagca ccagacgctc
     5521 attaccattt tgcacctcaa gagcatcgta cacgcgcggt acggcatcct gagggaattc
     5581 gacgtcaact acggcgccga ttacctggac aatctttcca gtagccatct taaatcctct
     5641 acgaaataac ctgtttaaac cgcggcggcc cccgagacga tctcggtgag ttcctgagta
     5701 atgctggcct gacgagcttt gttgtatacc aactgcagct ctttaatcag gctgccgcca
     5761 ttgtcggtcg cggctttcat cgccaccata cgggcggcct gctcgctggc caggttttca
     5821 accacgccct gataaacctg agattcgaca taacgacgca gcagggtatc cagcaacgcc
     5881 ttcggatcgg gttcgtacag gtaatcccag gatttatgtt tcagatcatc atcatctgat
     5941 gccggtaacg gcagcagctg gctgatggtc ggaacctgag acatggtgtt aataaatttg
     6001 ttgctgacaa tgtaaagctt gtccagacgg ccttcgtcgt aggcctgcaa catcactttt
     6061 accggaccga tcagttcgga cagggaaggg ttatccccca tgccggtgac ctgggcaaca
     6121 acattgccgc ccacggagtt gaagaacgac acgcctttcg agccgatcat tgcgaggtcg
     6181 cattgaacgc ctttgtcggt ccaggtcttc atttccgcca gcagtttttt gaacaggtta
     6241 atgttcaaac caccgcacaa accacggtcg gtcgacacca ccaggtagcc cacgcgttta
     6301 acgtcgcggt cttccaggta agggtgctta tattccagat taccgtgtgc aaggtgacca
     6361 atcactttgc gcatggtttc tgcataagga cggctggccg ccatgcgatc ctgcgattta
     6421 cgcattttgg aagcggcgac catctccatc gctttagtga tcttttgcgt gttctggacg
     6481 cttgcgatct tactacgtat ctcttttgcg ccggccatga gcttctcctc aatgccttgc
     6541 ggcctgccct aaggcaagcc gccagacgtt accaggattg ggttgctttg aaggaatcga
     6601 ggatgccttt cagcttgcct tcgatttcgt cgttgtagcc accggtctgg ttgatctctt
     6661 gcatcaacgg agcgtgatca cggtcgacgt aagccagcag agcggcttcg aagctgccaa
     6721 ttttcgacag ttcaacatcc gccaggtaac cacgttctgc tgcgaacaga accagagact
     6781 gctgcgcaac ggacatcggc gcatactgtt tctgtttcag cagttcggtc actttctgac
     6841 cgtggtcaag ctgcttacgt gttgcatcgt caaggtcgga tgcaaactga gagaacgctg
     6901 ccagttcacg atactgtgcc agagcggtac ggataccacc ggacagtttt ttcatgatct
     6961 tggtctgtgc tgcaccacca acacgggata cggaaatacc cgggttaacc gcaggacgaa
     7021 taccggcgtt gaacaggttg gtttccagga agatctgacc atcggtaatg gagattacgt
     7081 tggtcggaac gaacgcagaa acgtcacccg cctgagtttc gataatcggc agtgcggtca
     7141 gagaaccggt tttccctttc acttcacctt tggtgaaggc ttcaacgtat tcggcgttaa
     7201 cacgcgcagc acgctccagc agacgagagt ggaggtagaa tacgtcgccc gggaatgctt
     7261 cacgtcctgg cggacgacgg agcagcaggg agatctgacg gtaagcaaca gcctgtttag
     7321 acaggtcatc gtaaatgatc agcgcatctt caccgcggtc acggaagtat tcgcccattg
     7381 cgcaaccggc atacggagcc agatattgca gtgcagcgga ttcagacgcg gttgctacca
     7441 caacgatggt gttagccagt gcgccgtgct cttccagttt acgtaccacg ttagaaatgg
     7501 tggacgcttt ctggccgata gcgacataga tacatttgat accggaatcg cgctggttga
     7561 tgatggcatc gatagccagt gcggttttac cggtctgacg gtcaccgatg atcaattcac
     7621 gctgaccacg accaattggg atcatggagt caacggcttt ataaccggtc tgtaccggct
     7681 gatctacgga ctgacgttcg ataacgcccg gagcgattgc ttctacagca gagaagccgt
     7741 cgtgatccag cggaccttta ccgtcgattg gtgcacccag agtgttaacc acacggccca
     7801 gcaggccacg gccaaccgga acttccagga tacggccagt acacttaact ttcatgcctt
     7861 cggcaaggtc agcgtacgga cccataacaa ccgcacctac agagtcgcgc tcgaggttca
     7921 gtgcgatagc gtaacggtta cccggcaggg agatcatttc accctgcata caatcggcca
     7981 ggccgtgaat gcggataaca ccgtcactta cagaaacaat agtaccttcg ttgtgagctt
     8041 cactcacaac attgaactga gcaatgcgct gcttgatcag ttcgctgatt tcggtggaat
     8101 tcagttgcat gctccagtcc ccttaagact gcaagacgtc tgcaaggcgc tcaagacgac
     8161 cgcgtacgct gccatcaatg accatatcac ccgctcggat gataacgcct gccattacag
     8221 acttatcgat tttgcaattc agcttaactt tgcgtgacag acgtttttcc atcgcagcag
     8281 aaattttcgc gagctgttgt tcactcagtg cggcagcgga aatgacgtct acctcagcgg
     8341 tagcctcact cacggcacga aggtgaataa actgctccag aacatccggg agcgcgttaa
     8401 gacgaccatt ttcagccatt acccgaatca ggttctgacc gttttcgtcc agttgctcac
     8461 cacaaactgc gataaacgac tcggcgagcg tttctggcgc aagcgcgcca gagagaagct
     8521 ctgccatttg ttcgtttttg gttacctcgg cggcaaacgc cagcatgtcc tgccagcgtt
     8581 ctacactttg gtgttcgacg gcaaagtcaa aagctgcttt ggcgtagggg cgagctaccg
     8641 taataaattc agacatcagc ccctccctcc ttacagttca gcgacaagtt tatccacgat
     8701 gtcgctgtta gcagcttcat ccacggaacg ttcgatgatc ttctcggcgc cagcaacagc
     8761 caggatagca acttgcttac gcagctcttc acgggcacgt ttacgctcgg cttcaatttc
     8821 tgcctgcgcc tgggccacga ttttagtacg ttcctgttct gcctcagctt tcgcttcgtc
     8881 cagaatctgc gagcggcgtt tgttcgcctg ctcgatgatt acctgggctt ccgctttcgc
     8941 ttttttcagc tggtcggtcg cgctggcctt tgcaaggtca aggtccttat gtgctcgttc
     9001 tgcggaagca aggccgtcag caatttcttt ttgacgtttt tcgatggctg ccattaatgg
     9061 cggccatacg tacttcatgc agaacagaac gaacaggaca aacgcgatgg cctggccgag
     9121 gattgttgcg ttaagattca cagcacaatg cctctattta gttaacgttc tgatattgct
     9181 ctttaaataa aagcaacgct tactacgcga cagcgaacat cacgtacaga cccagaccta
     9241 cagcgatcat cgggatagca tccaccagac ccataacgat aaagaactga gtacgcagca
     9301 gaggaatcag atcaggttga cgcgctgcgc cttccaggaa tttacccccg aggatgccga
     9361 taccgatcgc agcaccgatt gccgccagac ccatcatcac agcggcagcc atgtacagca
     9421 gatccatatt caggttttcc atgacagtct ccagtttgtt tcagttaaaa cgtagtagtg
     9481 ttggtaaatt aatgttcttc agacgccatc gacagataga cgatcgtcag aaccatgaag
     9541 atgaaggctt gcagcgtaat gatcaggatg tggaaaatgg cccacggcac attcaggatc
     9601 cactgtgacc accacggcaa cagaccagca atcagaatga aaatcagctc accggcatac
     9661 atgttaccga acagtcgcaa accaagtgaa actggtttgg acagcaggct taccccttca
     9721 aggattaagt tgacaggaat gaacgcccag tgattgaacg gctgcagcgt caactctttc
     9781 gtgaagccgc cgatgccttt cattttgatg ctgtagaaca gaatcaggat aaatacgccc
     9841 aatgccatag acagcgttac gttcacgtcc gcagacggaa ccacacgcag tgcaggcaga
     9901 cccagtacat gttcagcaat gtacggcagc aggtcgatag gcagtaaatc catcaggttc
     9961 atcaggaata cccagacgaa gatcgtcagg gccagcggag caatcagctt gcttttgcca
    10021 tggtacatgt ctttcacgct accattaaca aagccgatca ccagctcaat cgcggtctga
    10081 aacttacctg gcacaccgct ggtcgccttt ttggctacgc tacggaataa aaccaggaac
    10141 aacagaccca gcaccaccga gaagaacatg gagtcaatat tgattgtcca gaaggtggct
    10201 ggggggtttt gtggatccac cagcgagaat gtacgcaggt ccagctgaag gttattcagg
    10261 tggtgtccta tgtaatcctg cggcgtcata ttttctgaag ccatgatgcc ttttaccctt
    10321 tgttgttaat tacagccggt gccagtatct gaaccaccag caccaaaacc cacgtaacga
    10381 tcagcggcaa gaataccgcc tttaaaaccg ccaacgccac caccagtaac accaacatcg
    10441 ccagaacttt gaaagcttcg ccaaatgcga atgtccaggc cacccggcct ttcgctggtg
    10501 tatgcgcctg gtgacgccag gcaaatatca taaacaaaac gttaggcaga aagactgcca
    10561 ggcccccgct tattgcagag acgccccaga aggggtcttt gaggctgaac agcaatccac
    10621 ttgctatcac caccagtaac tgaacgagca gaagcttccg agcaacgttt cgactcacga
    10681 gcgacacaga catcacgttt ttcactcctg ctcccttcga ggtatgccgc gtgtcgtata
    10741 aaactttctt taaggcttag agtcaagcat caaaaagcgg tcaaattata cggtgcgccc
    10801 ccgtgatttc aaacaataag tagccaaaag gtgaataaat gtttaaatat ttttccagtg
    10861 catacaattg cgacttttct gctaaccctg ttcgatcatg aaaaactgta aataacgcgt
    10921 aaacactggt gataaagcgt gcttcagatc acatattgcg catgttcgcg cacagcatat
    10981 ttatttactt ggcaaatgat gcctttgcaa gtttatgata tttcagtcta aaaac
//
LOCUS       DBANUU010000141        10964 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000141 DBANUU010000000
VERSION     DBANUU010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.142"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            96..710
                     /gene="evgA"
                     /locus_tag="ACIE68_003836"
     CDS             96..710
                     /gene="evgA"
                     /locus_tag="ACIE68_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFX5910082.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            715..4308
                     /gene="evgS"
                     /locus_tag="ACIE68_003837"
     CDS             715..4308
                     /gene="evgS"
                     /locus_tag="ACIE68_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFX5910083.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4364..5509)
                     /gene="yfdE"
                     /locus_tag="ACIE68_003838"
     CDS             complement(4364..5509)
                     /gene="yfdE"
                     /locus_tag="ACIE68_003838"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFX5910084.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5583..6527)
                     /gene="yfdV"
                     /locus_tag="ACIE68_003839"
     CDS             complement(5583..6527)
                     /gene="yfdV"
                     /locus_tag="ACIE68_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFX5910085.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6597..8291)
                     /gene="oxc"
                     /locus_tag="ACIE68_003840"
     CDS             complement(6597..8291)
                     /gene="oxc"
                     /locus_tag="ACIE68_003840"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFX5910086.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8345..9595)
                     /gene="frc"
                     /locus_tag="ACIE68_003841"
     CDS             complement(8345..9595)
                     /gene="frc"
                     /locus_tag="ACIE68_003841"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFX5910087.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10108..10740)
                     /locus_tag="ACIE68_003842"
     CDS             complement(10108..10740)
                     /locus_tag="ACIE68_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFX5910088.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         3214 a         2462 c         2259 g         3029 t
ORIGIN      
        1 agattttctt atttttattg agaaaatgag atgacgcctt atgtctgtat tactacaggg
       61 agaagggaga tgcttcattg caaagggaat aatctatgaa cgcaataatt attgatgacc
      121 atcctcttgc tatcgcagca attcgtaatt tattgatcaa aaacgatatt gaaatcttag
      181 cggagttgac tgaaggggga agcgccgttc agcgggtgga aacacttaag cctgatatcg
      241 tcatcattga tgtcgatatc cccggagtta acggtatcca ggtgttagaa acgctgagga
      301 agcgccaata tagcggaatt attattattg tctccgctaa aaatgaccat ttttacggga
      361 aacattgtgc tgatgctggc gctaatggat tcgtgagtaa aaaagaaggc atgaacaata
      421 tcattgcggc tattgaagct gcaaaaaatg gctactgcta tttccccttc tctctcaacc
      481 ggtttgttgg aagtttaacg tccgaccagc aaaaactcga ctccttatcg aaacaagaaa
      541 ttagtgtcat gcggtatatt cttgatggca aggataataa tgacattgct gaaaaaatgt
      601 tcatcagcaa caaaactgtc agcacttata aaagtcgcct gatggaaaaa ttagaatgta
      661 aatcactgat ggatctttac acattcgcgc aacgtaacaa aatcggctaa tcacatgaag
      721 tttttaccct atttttttct tctctgttgt ggtctttggt cgaccataag tttcgcagac
      781 gaagattaca tcgaatatcg tggcatcagt agtaacaacc gtgtcacact tgatccagta
      841 cgtctgagca ataaggaatt acgttggtta gcgagcaaaa aaaatcttgt gattgcagta
      901 cacaagtccc aaacggctac cttgttgcat accgattcgc agcaacaggt tcgtggtatt
      961 aatgctgatt atttaaatct tttaaaaaga gcgttaaata tcaaattaac actccgggaa
     1021 tacgcagatc atcaaaaagc aatggacgcg cttgcagaag gtgaagtcga tatagtgtta
     1081 tcacatttag ttacttcgcc gcctcttaat aatgacattg ctgcaaccaa accattgata
     1141 attacctttc cggcgctggt aaccaccctt cacgactcaa tgcgaccgct tacctcacca
     1201 aaaccagtaa atattgctcg ggtagcaaat taccccccag acgaggtaat tcatcaatca
     1261 tttccaaaag caacaattat ctcttttaca aatttatatc aggcattagc atccgtctca
     1321 gctgggcaca atgattactt tattggtagt aacatcatta ccagcagtat gatttcccgc
     1381 tatttcactc actccttaaa tgtagtgaaa tattataact cgccgcgtca atataatttt
     1441 ttcttgacca gaaaagaatc tgtcattctt aatgaagtac tcaatagatt tgttgatgct
     1501 ttaacaaatg aagttcgcta tgaagtatca caaaattggc ttgatacagg aaacctggcc
     1561 tttctgaaca aaccattaga actcactgaa catgaaaaac agtggattaa gcagcatccc
     1621 aatttaaagg tgctggaaaa tccttactcg cccccctatt ctatgacgga tgaaaatggc
     1681 tcggttcggg gcgttatggg ggacattctt aatattatta ccttgcaaac aggtttaaat
     1741 ttttctccga tcaccgtttc acacaatatc catgctggaa cacagcttag ccccggagga
     1801 tgggatataa tacctggcgc tatttatagt gaagatcgag aaaataatgt tttatttgct
     1861 gaagccttca taacaacgcc ttacgttttt gtcatgcaaa aagcgcctga cagtgaacaa
     1921 acattaaaaa aaggaatgaa agttgccatt ccatattatt atgagctgca ttcgcaatta
     1981 aaagagatgt atccggaggt tgaatggata caggtcgata atgccagcgc tgcatttcac
     2041 aaggttaagg aaggtgaact tgatgctctg gtcgcgacac agttaaattc gcgttacatg
     2101 atcgatcatt actatcctaa tgaactttat cattttctta ttcctggcgt tccgaatgca
     2161 tcgctttcgt tcgcttttcc tcgcggagaa ccggaactta aggatattat taataaagca
     2221 ctgaatgcaa ttcccccaag cgaagttctg cgcctgacgg aaaaatggat taaaatgccc
     2281 aatgtgacca ttgacacatg ggacctatat agcgagcaat tttatattgt tacgacatta
     2341 tccgttttat tagttggcag tagcctttta tggggattct acctgttacg ctcagttcgt
     2401 cgtcgtaaag tcattcaggg tgatttagaa aaccaaatat cattccgaaa agcactctcg
     2461 gattccttac cgaatccaac ttatgttgta aactggcaag gtaatgtcat tagtcataat
     2521 agtgcttttg aacattattt cactgcggat tactacaaaa atgcaatgtt accattagaa
     2581 aacagtgact caccctttaa agatgttttt tctaatgcgc atgaagtcac agcagaaacg
     2641 aaagaaaatc gaacaatata cacacaggta tttgaaattg ataatggcat cgagaaaaga
     2701 tgcattaatc actggcatac attatgcaat cttcctgcaa gtgacaatgc agtatatatt
     2761 tgtggttggc aagatattac tgaaacgcgt gatctaatta atgcactcga ggtagaaaaa
     2821 aataaagcga taaaggctac cgtagcaaaa agtcagtttc tggcaacgat gagtcacgaa
     2881 ataagaacac caataagctc tattatgggc ttcctggaac ttctgtcggg ttctggtctt
     2941 agcaaggagc aacgggtgga ggcgatttca cttgcctacg ccaccggaca atcactcctc
     3001 ggcttaattg gtgaaatcct tgatgtcgac aaaattgaat cgggtaacta tcaacttcaa
     3061 ccacaatggg tcgatatccc tactttagtc cagaacactt gtcactcttt cggtgcgatt
     3121 gctgcaagca aatccatcgc attaagttgc agcagtacat ttcctgatca ttacctggtt
     3181 aagatcgatc ctcaggcgtt taagcaggtc ttatcaaatt tactgagcaa tgctctcaaa
     3241 tttaccaccg agggggcagt aaaaattacg acctccctgg ttcacattga tgacaaccac
     3301 gctgttatca aaatgacgat tatggattct ggaagtggat tatcgcagga agaacaacaa
     3361 caactcttta aacgctacag ccaaacaagt gcaggtcgcc agcaaacagg atctggctta
     3421 ggcttaatga tctgcaaaga attaattaaa aatatgcagg gcgatttgtc attagaaagt
     3481 catccaggca taggaacaac atttacgatc acaatcccgg tagaaattag ccagcaagtg
     3541 gcgactgtcg aggcaaaagc agaacacccc atcacactac ctgaaaagtt gagcatatta
     3601 atcgcggatg atcatccgac caacaggcta ttactcaaac gccagctaaa tctattagga
     3661 tatgatgttg atgaagccac tgatggtgtg caagcgctac acaaagtcag tatgcaacat
     3721 tatgatctgc ttattactga cgttaatatg ccgaatatgg atggttttga gttgactcgc
     3781 aaactccgtg agcaaaattc ttccttaccc atctgggggc ttacagccaa cgcacaggct
     3841 aacgaacgtg aaaaagggtt aagttgcggc atgaacttat gtttgttcaa accgttgacc
     3901 ctggatgtac tgaaaacaca tttaagtcag ttacaccagg ttgcgcatat tgcacctcag
     3961 tatcgccacc ttgatatcga agccctgaaa aataatacgg cgaacgatct acaactgatg
     4021 caggagattc tcatgacttt ccagcatgaa acgcataaag atctacccgc tgcgtttcaa
     4081 gcactagaag ctggcgataa cagaactttc catcagtgta ttcatcgcat ccacggtgcg
     4141 gctaacatcc tgaatttgca aaagttgatt aatattagcc atcagttaga aataacacct
     4201 gtttcagatg acagtaagcc tgaaattctt cagttgctga actctgtaaa agaacacatt
     4261 gcagagctgg accaggagat tgctgttttc tgtcagaaaa atgactaaat agcggctccc
     4321 acaatgttca aatgtgggag ctatttacca gcacatctta catttatgat gagaactcct
     4381 ggcgaatttg ttccccatgc tggtcgagcg ttgccgctcc cggcataaca tgcgggtccg
     4441 cgcagccgct gatttttatc ggattacccg gcatcattat tcccccggct tcaatcaaca
     4501 tatttcttgc ctgagtttgt ggcaatttaa tggcctcagc cacacttaat aacggcgcga
     4561 cgggtacacc aacttcatgt attctggcta accaaacttc agctgcctgc gtttttaacg
     4621 tccgctcaat atattgttta agaatagcct ggttttgtac gcgtaaaata ttgctgctaa
     4681 atcggggatc attaaccagt tccgtaagct ccagtgcctg gcataacgca gaaaaaagct
     4741 tgtcattacc acaacaaatc gtaatcggct tatcctgagt attgaaaaca tcaaaaggtg
     4801 ccatgtaggg atggcgattt cccagacgtt gtggtgactt ccctgtcgcg atatatgcca
     4861 tcagaccatg ctccagaaaa ctcagcgtgg catcaaacat cgctatatcg acatgcgccc
     4921 ctctctggct cttttcgcgg ccataaagtg cactcactat tccgctgaat aaatagacac
     4981 cgccgcatag atccgcaaga gatgtaccaa cgcgcactgg cggagcatca ggatatcctg
     5041 tttccatcat tatcccgctc attgcctgaa tgatggtatc gtaggcagga gcatctttta
     5101 gcggaccggt atgtccgaaa cctgacgatg aagcatatat gaggcgcggg ttgatttctt
     5161 gaagcgtttc ccatgaaaac cccagttttt ccattgtacc tgggcgaaaa ttctcagcta
     5221 atacatcagc ttgtttgagc atatttataa atatactttt atcgtgatca ttctttaaat
     5281 caagaaccac actctctttg ccatgattaa taaaactgta atagagtgac tgtccatcca
     5341 catagggacc aaatgtgcgg gtatcatcac catgacccgg cggctcaact ttaattaccc
     5401 ttgcgcccat attacaaaga agttgagttc cgaaaggtcc attaaggaca tgtgtcatat
     5461 cgataactaa taagccttca aacggccctt tgctttcatt atttgtcatt tgcttatgcc
     5521 ttgaaaaaag ccgaaggtga aaagacactt cggcatttat gtaaaatcaa taacatgatt
     5581 aattatgaaa ccagtcgact gacataaatc cacaagggag ccgtgacgac aaagcccagt
     5641 acgctcacag ccaatgacgc tgtaccagta cgggtgtaga cattaaaccg gctggcaata
     5701 atgattccgg agaatgccgg cggtaatgcg cctgccagta ccatcatctg cagatgttcg
     5761 ctgttcaaat gacatgccat accaacgaga agcagtgcca gtggcatcag aatcagcttc
     5821 aggaaggtgt tataagcaat ttcggcactg aactcgaatt tatgtgcagc cagagtcaac
     5881 cccgcagcga ataccgctac ccctgagtta gccttcgcaa tcagattaaa ggttgggtcc
     5941 catgctgccg gaatttttac cccaaccaac accaggatcg ttgccagaac aggtgcccat
     6001 actactggct cctttgccgc agaaattaat gcgctcagat tactattctt cttaccatcc
     6061 gctcctgagg aaggattcag caaatacaga ccaataggaa tagtaattgc gttaacaata
     6121 atagaaataa ttgctaccac taaacctgtt gatacggaat caccataaat aggatcgaga
     6181 actgcaaacc ccaggaatcc aatggtaggt gaacccgcaa ttaatgcaca tacagctgct
     6241 tctgcatggg tacgtttaaa aaatttgtag caaccaaacc aggagaaaaa gaaacatccg
     6301 acaataacca caagtgatac aagggtcaga cgagtgtccg caaaaatcat ttccctgttt
     6361 gcccgggtaa tagaaacaaa tagagccgca ggaagagcgt agtttaatac cagtttatta
     6421 aatgcgcggg cttgatcttc tgaaaaagtt tctcgtctgc cgctaaaata acccaacagc
     6481 atgattacga taataggcaa taaatcgcca ataaaaaatg ttagcataat agtatttccg
     6541 ataagggtcc cctcaccatc gcataatgag ttggcaagag gagacgatgt cagtatttaa
     6601 ttaccagcga cttgttttgg gttaagtttg gtaatatggc cactttcagt tcctgctgca
     6661 gggtcgatga ccacattaat aatggtcggt ttgcgcgact gaataccggt ggttaaagca
     6721 tgacgaagtt catctgtcgt ggtgacgtta tagccaacgc cacgaaacgc atccattaat
     6781 ttgtcatacc ttgcatggtg caacagatcc gttggtgatg gtgcaccagc gccactgaga
     6841 tcaacaccgt ctcctctgta gatgccgcca ttattaaaaa taacgatcgt caccggcagg
     6901 ttatatcgac aaatcgtttc aatttccatc ccactgaaac caaaagcact atcaccttca
     6961 atggcgacaa ccggagaacc agaagtcacg ctagcaccga tggcatagcc cataccgatg
     7021 cccatgacac cccaggtacc acaatccaga cgacgacgtg gtttatacat atcaataata
     7081 tttcgtgcat tatccagggt atttgcacct tcattaacta aataaatatc ctggttctcg
     7141 cgcaatacgt cgcgcacagc acttaatgca ttaaagtaat ttaatggttg agtatctgta
     7201 cttaattttt catgcatttt ttgtgcattt tgctgcttgt ggatatttaa aatatcgcgc
     7261 cataccagtg gagtcgtaaa tgtgttttgt ttcagttctg ccagcatacc ttgcatactg
     7321 gatgcaatat caccaacgac tggcacagca atggggcggt tgctgtcaat ttcctgcggt
     7381 tcaatatcca gttgaataaa ctgtgtatct gccgcccatc cttttttacc gtgtgccaat
     7441 aaccaattca gtcgtgcacc aacaagcatg acaacgtcag catttgccag ggcaaacgaa
     7501 cgcgcagctg ccgcagaaag tggatgcgta tcttcaagga tccctttcgc catagacatt
     7561 ggcaggaatg gaatctgagt actttcaata aattcacgaa gctgttcatc agcttgtgaa
     7621 tacgccgcgc ctttgccaag gataattaat ggccgttcag cttttgctaa aagcgaaatt
     7681 gcgctagtga ctgacttcgg gcatggcaat aatgctggcg acggattttc aactttaaca
     7741 atcgtggtta acgcttcgtc tttttccatc gtcgcggcca ggacatttgc tggcaaatca
     7801 agataaactc cgccagggcg acccgataca gagacccgga tagcgcgtgc caatgcaatg
     7861 ccaagatcct gcggctgatt aacgcgaaat gctgctttgg catacggttt tgccgcattc
     7921 atttggtcca gctcttcata atcaccttgc tgtaggtcga cgatcgcgcg gtcgctggag
     7981 ccgctaatca tgatcatcgg aaaaccattt accgttgcgt tggccaatgc ggtcaaacca
     8041 ttgaggaatc ctggcgcaga aactgtcagg cagatccccg gtttttgggt aagaaaaccg
     8101 cttgccgcag cggcatagcc tgccgactgc tcatgacgaa aaccaatata acgaatgcct
     8161 tccgcctggg catggcgtgc catatccgtc acaggaatac ctacaacacc ataaatagtg
     8221 tcaatattat tctgttttaa tgcttcaacg atgatatgca taccatctgt catttgaagt
     8281 tgatctgaca tgattaataa cctcgaaatt tttataaatt gggagccaga agcccccgtt
     8341 aatatcagat ggcgtggttt tgcttcattg cagcaatttc atcgtcacta taacccagct
     8401 cctgcaatac agcagcggta tgttcaccta atagcggcgc agctttaata tccggcgtaa
     8461 aggcagagaa tttcattgga caaccaacgg tcagatattt tccacgcaac ggttgttcca
     8521 cttcaacaac actgccactt tggcgcaaag agggatcaag tgaaatttct ttcatactta
     8581 aaaccggtgc acaaggaata tcaaactgag tcaaataggc caccgcttca tgtttatcaa
     8641 tagtgacagt gtatttttcg atttcagcaa aaatatcgaa aatatgtggc tgtcgtgcat
     8701 gggctgtact gtatgccgga tcggtaatcc attctggttt gccgatggct ttacaggtgt
     8761 tttcccagtt ttgctcctga atagtgaaat aaatataggc gttaggatcg gtttcccagc
     8821 ctttacattt caggatccag ccaggctgac cgccaccacc tgcattacca ccgcggggaa
     8881 ctgcatcacc aaatgtacca ttcggatact gcgggtattc ttccagataa cccaatttat
     8941 cgagacgctg ctggtcacgt aatttcacgc ggcaaaggtt caatacggca tcctgcattg
     9001 acatggtgac tcgttgccca cgccccgttt tttcgcgatg cagcaaagca gcaagtaaac
     9061 cgatcagcaa atgcattccg gtgttgctgt cacccaacgc tgcagcgctt accagcggcg
     9121 gaccatccca aaaacccgta gtggatgccg cgccacccgc tgcctgagca acgttttcat
     9181 aggcttttac attcacataa ggcgaacact catcaaaccc tttgatcgaa ccaaaaatca
     9241 gacgtggatt gatttcttga atatgctccc aagtgaagcc catgtgatca atggcccctg
     9301 gatgaaagtt ctcgactaag atatcagctt cgcggatcag cttttccatt acctctttgc
     9361 cttccgctgt tttggtattt aactcaatag aacgtttgtt actgttaagc atggtgaagt
     9421 aaagcgcatc gatatcagga atatctcgca gctggtgacg cgttacgtca ccaacgccgg
     9481 gacgttcaat tttaataacg tcagcgccaa accaggccag catttgagta caagatgggc
     9541 cagattgcac accggtgaaa tcgagaactt taattccttg aagtggagtt gacatagcaa
     9601 tacctattag ttaattaaat atgtgttaat ttagcgttta tttaaggcgt aatatcttct
     9661 ataaataacc cgcaaaatat cgtcgtagct gcactcgata tttttaaaat cctgtatcaa
     9721 gtgctgaaaa tcatttcatc gccacttcca attcctttct gcatggtaag tagtgaggac
     9781 gaaaagaaga atatgataaa tattgattcg tggtaatcag agagagtcgc gacatttgtg
     9841 atgttattct tacaactgta agaagcgccg gtgatttaca agtaagggta tgattacgct
     9901 ttttaaagcg taccataaaa cataacataa taaatgattt gtgatgacac gggattattg
     9961 ggatttactt tgaggaagat aaaatgcgtg tttatagaac cgcgagcgcc aaataatttt
    10021 atgaccacaa cctgtcgtat tgtggtcata aaaaattgtc ggctggaggg agttaaatcc
    10081 tttacctcaa tatactgcct gtcagcatta gttcacaaac agtgcttcgc tgtcgacgat
    10141 gattccatct tccgcacctt taagcgccag gtttgcctcg tagtattgct ttttatcaag
    10201 cagtttctgc gcatcagcaa gtgcattacg agtctgttta agcggcatca gatattggtt
    10261 ttccattacg ccaacaccgg ccaggcgaag ttcttccatt gcgccctttt tatccccttt
    10321 cgccattttt tcattggcaa tttttatcgc ggcttctttt tcgggagttg ctacataact
    10381 ctcagaaatt ccgacagatg cattaatgac aatatattgg tcatcattga gattggtctt
    10441 tttacctggc ttagcgaact ttgcccattc ggtactgtca tctgacagca aagcggaagc
    10501 ttcattggtc agttcttttg ccttttctgg atcgccatga aacagagcca ggcgcgctac
    10561 ctgcacgtca cgcatcgcat ataaaccttg ttcagagata cgctctgctg ctgccgtttt
    10621 ctgggtttgc tgaacttgct gagtttgctg ttgtgccgct accggtgcat tatcagcagc
    10681 ccatacagtt gaagatgcca gaattgctgt gaccatcgta gccataatta aacgtttcat
    10741 acttacctca ttctttaaca gttttagaaa acgccgccat catattgggt gagcatgaaa
    10801 tgagaaatca gagagcaacg gtcattttga aagggttatc ttacagttgt agttattatc
    10861 gcaagaagta ttttagtctt acaattgtag gaatctgctt cctgtcgcag agaactattc
    10921 ttatgtacca caaagagatg tcatttatta tatcgatgta aata
//
LOCUS       DBANUU010000142        10894 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000142 DBANUU010000000
VERSION     DBANUU010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.143"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            93..1133
                     /locus_tag="ACIE68_003843"
     CDS             93..1133
                     /locus_tag="ACIE68_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFX5910089.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1165..2358)
                     /gene="lplT"
                     /locus_tag="ACIE68_003844"
     CDS             complement(1165..2358)
                     /gene="lplT"
                     /locus_tag="ACIE68_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFX5910090.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2351..4510)
                     /gene="aas"
                     /locus_tag="ACIE68_003845"
     CDS             complement(2351..4510)
                     /gene="aas"
                     /locus_tag="ACIE68_003845"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFX5910091.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDELGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5096..6127
                     /gene="galR"
                     /locus_tag="ACIE68_003846"
     CDS             5096..6127
                     /gene="galR"
                     /locus_tag="ACIE68_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFX5910092.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTSNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6134..7396)
                     /gene="lysA"
                     /locus_tag="ACIE68_003847"
     CDS             complement(6134..7396)
                     /gene="lysA"
                     /locus_tag="ACIE68_003847"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFX5910093.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7518..8453
                     /gene="lysR"
                     /locus_tag="ACIE68_003848"
     CDS             7518..8453
                     /gene="lysR"
                     /locus_tag="ACIE68_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFX5910094.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8440..9132)
                     /gene="ygeA"
                     /locus_tag="ACIE68_003849"
     CDS             complement(8440..9132)
                     /gene="ygeA"
                     /locus_tag="ACIE68_003849"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFX5910095.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(9261..10679)
                     /gene="araE"
                     /locus_tag="ACIE68_003850"
     CDS             complement(9261..10679)
                     /gene="araE"
                     /locus_tag="ACIE68_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFX5910096.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         2801 a         3001 c         2639 g         2453 t
ORIGIN      
        1 tccggctggc cttaagattt ttctctttcc cttttccctt ccctctgcca tttttatatt
       61 ccttatgtcg tgattataaa aaggaaacgg ctatgcaata tcaccgtata ccccacagtt
      121 cgctggaagt cagcacgctg gggcttggca cgatgacgtt tggtgaacag aacagcgaag
      181 ccgacgccca cgcacaactc gactatgccg tcgctcaggg cattaacctt atcgacgttg
      241 ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg gttaaccgaa acctacgtcg
      301 gcaactggct ggcgaaacat ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg
      361 gaccgtcgcg caataatgac aagggcatcc gcccggatca ggcgctggat cggaagaata
      421 tccgcgaagc gctgcatgac agcctcaagc gcctacagac tgattacctc gatctttatc
      481 aggtgcactg gccgcagcgc ccgaccaact gcttcggcaa actcggttat agctggacgg
      541 attctgcgcc tgcggtttcg ctgctggata cgctggacgc actggcagag taccaacgcg
      601 cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc atttggcgta atgcgctacc
      661 tgcatctggc ggacaaacac gatctgccgc gtattgtcac cattcagaac ccttacagtc
      721 tgttaaaccg cagttttgaa gtaggtctgg cagaagtcag ccagtatgaa ggggtcgaac
      781 tgctggccta ttcgtgcctg ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa
      841 aacccgctgg cgcacgtaat acgctcttta gtcggttcac ccgctatagc ggtgagcaaa
      901 cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg tcatggcctg gaccctgctc
      961 agatggcgct cgcgtttgta cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa
     1021 ccacgatgga tcagctgaaa actaacatcg aaagtttgca tctggagtta agcgaagacg
     1081 tattagctga aattgaagcg gtgcatcagg tttatactta tccggcacca taagattaac
     1141 gccggttaaa accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat
     1201 tgccagcgca aacagcgcac cgaagccaat gccaatgggc acgaccggga tgcctatcat
     1261 taccgccagc gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg
     1321 tactgcaatc gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt
     1381 gagcggaacg acaaaaaagc cccccatcac gccaatcagc atcaacaagg cataggctgg
     1441 cagcagctcg tgttgcaggg aaaaaatcag taccaccacg ccaatcaaaa tcccggctgg
     1501 catacagcgt gacacggttt ccagcgtaac taacttcgct gccgcacctg cgccaaccac
     1561 gataccaatc gctaccatcg cgttgagata ggtgggcgta gcgttatcgg taatgcccag
     1621 cgccaccggt acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa
     1681 actggtgccc accagcgaaa aacgcgtttc accattgcgc catagcgagg tgcaggcatt
     1741 caggaaactg cgggtcatgt tgatgagatt ccaggactgc cccggacgcg ccgccgccag
     1801 tttgggaatg tagatattgg caacgaccgc accaccgtag gccagtgcgc atgcggccag
     1861 ggcgacgagg acatgccagt cagccagcac accaccggct acggaaccga gcaaaatcgc
     1921 cgctatggta gaagcttcca ttaaaccgtt agctttcact aacttactac ccgtggttaa
     1981 ttcgccgaga ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt
     2041 atagccgaga aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc
     2101 gttggcaaac atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg
     2161 cgcaaaaaga atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg
     2221 atagaactgc gctttcagta acgccagagt ggcaaacagt agggcattat cgccaaacgc
     2281 agagagaaac tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt
     2341 gtgcactgac tcactcatcg tgttgttccg cttcgtctac ccagcttttc aacgtgacaa
     2401 agtcaggttt gccgctgcca agtaatggca tctgtttcag atagcgaata tcgcgcggta
     2461 cagcaagctc cggcacgccg tgctcgcggg catactgttg caacttatcg cgcgtcagtt
     2521 cgttatctgt ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag
     2581 tggcatggac tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca
     2641 ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccca
     2701 gctcgtcaaa acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa
     2761 cattctcggc ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca
     2821 ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc gataccaggg accgacaaca
     2881 ggcgcgcatc cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta
     2941 cgttgataga aacgacaggc gcgcattcgg tcacaccgta gccttcaagg atgcgcaggc
     3001 caaatttatc ctgccaaagc tgtttggtac tttcttgtag tttttctgcg cctgccacca
     3061 catagcgtag acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag
     3121 tcgaagtgcc gaacaacacg gtgcaactgc ggtcatacac caactccggc acaatacggt
     3181 aatgcagcgg gcttggataa agaaacactt ctgcacctgt gagcagtggc gtaaacaggc
     3241 ctaccgtcag cccaaaggag tgaaacagcg gtaacgccga cataaagcga tcgttggtgg
     3301 tgaagtcggc aatcgtttta atctgctcga cattcgccag aatgctttta tggctatgga
     3361 cgacgccttt cggatggcct tcagaaccgg aggtaaaaag gatcagcgcc tcttcttccg
     3421 gctgctgttt aacctgtgcc agacgcggca tcagcaaatg agcgaagatc catactttgt
     3481 cggcagtggt gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt
     3541 gctccggcag atgccagagt ttgcctttat cgagaaactg gcgggaagtg aagatggttt
     3601 tgatttcagc cgccgtaata gcactggtca gcccttttac cccggcagtg tagttcatca
     3661 ttgcgggaat gcggcgacgg gcgatggccc caaaaatcac tgccgcactg atgcctgcat
     3721 tgggcagcat taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa
     3781 caaacagcgt tttcgtaagc aatttgcgat aggagtctgg ggtaaagttg acgtcttcga
     3841 cacatttctt cccggctccg aagcggtaca ttgcactcag taaagattcg tacagcgttt
     3901 cacgcgggcg caccgccatt cgcgcttcca tcattatttg atgcagcatt tcgccagcga
     3961 ttttgcgacg gtcacgggca cgcggcgcat ccggcatctc cacctgcgtt ggtggcaaaa
     4021 tatgcagagt aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt
     4081 gcgtgagttc cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga
     4141 caaaacccgc gccatcgtag attttcatca gcgagcctgt agtggtgatg cgtccttcag
     4201 ggaaaatcac cactggtcgg ccctgttcca ccagacgtac cagatgttta atagccatag
     4261 gttgcgtcgg gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact
     4321 gttggcttat tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca
     4381 aaatgccatc aataaaagag acgtgattag gcgtaattag aacgcgctcg cccttcagtg
     4441 cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc
     4501 taaaaagcat tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca
     4561 cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc
     4621 aggttggtgc aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct
     4681 ctgggatctt gattgtggtc tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat
     4741 gaagggaaat gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccggca
     4801 aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac
     4861 ttcacctatc aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc
     4921 ggtcaatcgc aacagcgttt cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc
     4981 ttacttattt gcgtttggct cacattccca cgatgaaaac acgccacccc ttgaaccaac
     5041 gggcgttttc cgtaacactg aaagaatgta agcgtttacc cactaaggta ttttcatggc
     5101 gaccataaag gatgtagccc gactggcagg cgtttcagtc gccaccgttt cccgcgtcat
     5161 taataattca cctaaagcca gtgaagcttc ccggctggct gtgcatagtg caatggagtc
     5221 tcttagctat cacccgaacg ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt
     5281 cggtctgatc gttggtgatg tttccgatcc gtttttcggt gcaatggtga aagcggtcga
     5341 acaggtggct tatcacacca gtaatttctt attgattggc aacggttacc acaacgaaca
     5401 aaaagagcgt caggctattg agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca
     5461 tgccaaaatg atcccggatg ccgatttagc ctcattaatg aaacaaatgc ccggtatggt
     5521 gctgatcaac cgtatcctgc ctggctttga aaaccgttgt attgctcttg acgatcgtta
     5581 cggtgcctgg ctggcaacgc gtcatttaat tcagcaaggt catacccgca ttggttatct
     5641 gtgctctaac cactctattt ctgacgccga agatcgtctg caagggtatt acgatgccct
     5701 tgctgaaagt ggtattgcgg ccaatgaccg gctggtgaca tttggcgaac cagacgaaag
     5761 cggcggcgaa caggcaatga ccgagctttt gggacgagga agaaatttca ctgcggtagc
     5821 ctgttataac gattcaatgg cggcgggtgc gatgggcgtt ctcaatgata atggtattga
     5881 tgtaccgggt gagatttcgt taattggctt tgatgatgtg ctggtgtcac gctatgtgcg
     5941 tccgcgcctg accaccgtgc gttacccaat cgtgacgatg gcgacccagg ctgccgaact
     6001 ggctttggcg ctggcggata atcgccctct cccggaaatc actaatgtct ttagtccgac
     6061 gctggtacgt cgtcattcag tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag
     6121 cgactaaccg cagttaaagc aattccagcg ccagtaattc ttcgatggtc tggcgacggc
     6181 gaatcaaccg cgcctgacca ttatcaaaca gaacctctgg taacagcgga cggctattgt
     6241 agttggatga cattgatgcg ccatatgccc ctgtatcatg cagtaccaga taatcacctg
     6301 ccttcacttc cggcaaggcg cgggtttcaa catttccccc ttcctgctgg gtaaagacat
     6361 cgcccgattc acataacggt ccggcgacga cggtttccac cgttggcgcg tgttccagag
     6421 aacgaccatc agctgccagg gcactgatat ggtgataact accgtacatt gccgggcgca
     6481 tcagatcgtt gaacccagca tcaaccagca caaagtggcg gctacccatt tgtttgacgc
     6541 tccgcacctg agtaattaat acgccagact gcgctaccag gaagcgaccc ggttcaattt
     6601 ccagtttcac agggtggccc aaatggcggg cgatttgctc acgcgcggca ttccacagac
     6661 cataataatg ttcggtatca accgcctctt caccctgttg ataaggaaca gaaagcccac
     6721 cgcccgcaga aatagcctgt aaatcctgac cgaattcgat gacctgacgc accatagcac
     6781 cacacacctg ttccagatgg gcataatcaa cgccagaacc aatgtgcatg tgaatgccga
     6841 ccagctgcag atgatgacgt tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga
     6901 taccgtgctt gctgttttcg ccaccggtat tggttttttg gctatgtccg tgaccaaacc
     6961 ccggattaac gcgcagccat acccgatgcc ctggcgaaac ctggcccagt tggtcgagca
     7021 tatcaacaga acccgcattc accggaattt gcaattcact gacgcgttca agcgtcgcct
     7081 gatcgataac atctgccgta aaaacaatat catcggggtg cgtttgcgga ttgtaacccg
     7141 ccgccaacgc acgctctatt tcgcctaacg agacggaatc cactttcacg ccctgctcac
     7201 gcattaagcg caaaatatga atattggaac aggctttctg tgcaaagcgc accacatcaa
     7261 actgtttcag cgctgcaatc tgccgacgaa taatttgcgc atcgtagacc cacaccgggc
     7321 agccaaattc agcgggcaaa cgcagcagat tttcggcggt gagatcggta tcggtgctga
     7381 acagtgaatg tggcataaca aactccagat aagtgctttt ttatgattac gccacatcat
     7441 aaaaagaata aaaaatatcg atttatgtcg agtttatgca aaaatgatat ggattaccgg
     7501 attgcgagag agcgctaatg gccgccgtta acttacgtca tattgaaatt tttcatgcgg
     7561 taatgaccgc cggaagcctg actgaggcgg cacacctgct acacacctca cagccaaccg
     7621 tcagccgcga actggcgcgc tttgagaagg tgatcgggct gaaattgttt gagcgcgtac
     7681 gtgggcgatt acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct
     7741 ggtacggact ggatcgcatt gtcagcgccg cagaaagtct gcgcgagttt cgccagggag
     7801 aactgtctat tgcctgcctg ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac
     7861 cctttctggc acgttatccc gatgtcagct taaatatcgt gccccaggaa tcaccgctac
     7921 ttgaagagtg gctctcggcc cagcgtcatg atttaggact cactgaaacg ctccatacgc
     7981 ctgcgggaac agaacgtacc gaattactct ctttagatga agtgtgtgtg ttacctccgg
     8041 gtcatccgct ggcggtaaaa aaggtattaa cgccggatga ttttcagggt gagaactaca
     8101 tcagcctttc ccgtactgac agctatcgcc agttgctgga tcagctattt actgaacatc
     8161 aggttaaacg acgcatgatc gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac
     8221 gggcgggggt aggtgtttcg gtggttaacc cgctcaccgc actggattat gcggcaagcg
     8281 gtttagtggt gcggcggttc agtattgcgg ttccgttcac cgtcagcctg atccgccccc
     8341 tgcaccgccc gtcatcagcg ctggtgcagg cgtttagtga gcatttacaa gcggggttac
     8401 cgaaactggt cacttctctt gacgctattt tgtcgtcagc tacgacagca taaaagcgac
     8461 agcatcctcg gcatggatcg ccgcggtatc aaacacaggc aaaacactgc gctcttctgg
     8521 caccagtaaa ccaatttctg tgcagccaaa aatgacgccc tgtgcgccct gttctgcaag
     8581 gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc ccagacacag
     8641 ttcttcaaaa ataatctgat taattttcgc ccgttcatcc gcttcaggaa taagacaatt
     8701 gatggaaaat tgttccgtca gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt
     8761 acccagcagc gccacacgag tcattccggc cccggtaatt gcacgtccgg tggcatccgc
     8821 aatgtgtaag aaaggcagag tgcaacgtga ctcaatggca tccgccactt tatgcatcgt
     8881 attggtacat aacacaatac cttctgcgcc cgcccgctgt aagccaagcg ccgcctcagc
     8941 cagaatgtcc ccggttttat cccattcccc gcgacgctgg cactcttcta tttcatgaaa
     9001 atcgacgcta tgtagcagca cttgcgcaga gtgaagccca ccaagccgct gtttaatgcc
     9061 ttcatttatc aaacgatagt aaggaatggt ggattcccag ctcattcctc ccagcaaacc
     9121 aattgttttc attcctctct cctgatgtgt gttaccgcaa caaggctgaa taaggaacga
     9181 gacaaacgcc tcaacggcca agtgcccaat ctctattaac gaaaaaaggg ccggatgtac
     9241 agcacatccg gcccgtgaaa tcagacgccg atatttctca acttctcgcc tgccatcagt
     9301 ttgcgttcga tatgttccag cgtgacattt ttggtttccg gaatgagcca gaaagtaatg
     9361 cccacaaacg caatgttcag cgcagtgtag agccagaacg taccggcagc gccaatgcta
     9421 tcaagcagtg tcaggaaggt cgcgccgata atcatattcg acacccagtt tgtcgtcgtc
     9481 gaacaggtaa taccgaaatc gcggcatttc agcggctgaa tttcagagca caggatccac
     9541 accactggcg cggcgctcat cgcataaccg gcaatacaca tcatcgtcat gccaacagag
     9601 agccaggaca agccactgga agccgtaccg ttatcaaact gcatcaggca atagcccagc
     9661 accagagtgc ctaacgccat cacgctgaaa ccaattttca gagccggttt acgacctgct
     9721 ttatctaccg taaagaccgc aatgaaggtc gcgaacataa aggtcagtcc cacgaccaga
     9781 gtcgcaatca tctgttgttc tgtggtcgta aagcccgcca ttttgaagat acgcggcgcg
     9841 tagtacatga tgatgttcat accggtaaac tgctgcatcg cctgcaacaa cataccgaga
     9901 aacacagcac gacggacgtt acggttgatc ttaaacagtg cccaaccgcc ctgttttaac
     9961 ttcaggcttt cacgaatttc gttgagttct tctcgcgctt tttccgacgt atcgcgcagc
    10021 atacgcaata cttcttccgc ctcaatatga cgcccctttt ccgccagcca gcgcgggcta
    10081 tttggcagga agaccaccag aataatcagc agaactgctg gtaaagcaag aacccccaac
    10141 attgcgcgcc agttaccgct ataactgaac gctgtatcgg ataaaaacgc cagcacgatg
    10201 ccgagtgtga ccatcaactg gtacatactg atcatcttac cgcgaacgtt ttcacttgcc
    10261 atttcagaaa gatacagagg agcggtgtaa gacgcgatcc cgacagcaat gcccagcacc
    10321 acccgagcgg cgattaacat ctctacgctg gtcgcaaaag cggaccctat agaaccgagt
    10381 acaaacagga tggcccccgc catcaggctg tatttacgcc ccaggcggaa cgacagccaa
    10441 ccattaaaca gcgcaccaat tgctgcgccg agcatcatgc tactaaccac ccattcctgc
    10501 aaacgactgg tcagcacaaa gtgatcggta atgaacggca acgctccggc gattacgccg
    10561 atatcaagac caaataacaa tcctgcgacc gcagcagcta ccgaaacaaa catattcata
    10621 cgccgcgtat cccgcaaaga acgtggcgtt aaagcagatt ccgtattgat agtaaccatt
    10681 ttttcctgcc agcagagaat aagacatagt gaaaaaatac gtgaacaatt cacacaggtg
    10741 tcaggtcgga aatagcataa atatggatta aattgctacg acatgccgtt atgtgatgga
    10801 tattccaatt ttaaaattaa gttgaattat tgagattatt attaaccacc taattttaaa
    10861 gcagataaaa ttcataaagt tcattcattg ataa
//
LOCUS       DBANUU010000143        10890 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000143 DBANUU010000000
VERSION     DBANUU010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.144"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(204..1886)
                     /gene="glcA"
                     /locus_tag="ACIE68_003851"
     CDS             complement(204..1886)
                     /gene="glcA"
                     /locus_tag="ACIE68_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFX5910097.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
     gene            complement(2241..4412)
                     /gene="glcB"
                     /locus_tag="ACIE68_003852"
     CDS             complement(2241..4412)
                     /gene="glcB"
                     /locus_tag="ACIE68_003852"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFX5910098.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQLERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGAQVIAWVRRFLDESLPLEKGSYQDVVAFKVVDKQLRIQLKNVKETTLRTPAQFVG
                     YRGDTAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRVETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMFSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4434..4838)
                     /locus_tag="ACIE68_003853"
     CDS             complement(4434..4838)
                     /locus_tag="ACIE68_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFX5910099.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(4843..6066)
                     /gene="glcF"
                     /locus_tag="ACIE68_003854"
     CDS             complement(4843..6066)
                     /gene="glcF"
                     /locus_tag="ACIE68_003854"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFX5910100.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQLVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPTIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            complement(6077..7129)
                     /gene="glcE"
                     /locus_tag="ACIE68_003855"
     CDS             complement(6077..7129)
                     /gene="glcE"
                     /locus_tag="ACIE68_003855"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFX5910101.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7129..8628)
                     /gene="glcD"
                     /locus_tag="ACIE68_003856"
     CDS             complement(7129..8628)
                     /gene="glcD"
                     /locus_tag="ACIE68_003856"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFX5910102.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            8874..9638
                     /gene="glcC"
                     /locus_tag="ACIE68_003857"
     CDS             8874..9638
                     /gene="glcC"
                     /locus_tag="ACIE68_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFX5910103.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            complement(9645..10787)
                     /gene="yghO"
                     /locus_tag="ACIE68_003858"
     CDS             complement(9645..10787)
                     /gene="yghO"
                     /locus_tag="ACIE68_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFX5910104.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHCERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
BASE COUNT         2577 a         3052 c         2728 g         2533 t
ORIGIN      
        1 catcaagcga acataattaa tacagacttg ttttgcgtga taaaaaaaat agatgttctc
       61 acgctcttaa ttatttagca ggttatctga acacaaaaca ttattgccgc atggataata
      121 aagcgagaaa taattttcaa ttaataagac agaataatgt aaatgaagcc ggatgatatt
      181 aacgatcatc cggcattatt gatttacgag accaacattc cagtaaacac atacgcctgc
      241 aacaacgtga taacgccgat aacgctggca aaaatcagac tgtgcttcac ggtgtagcgg
      301 aatagttcag attctcggcc caccatgccc gtcgcggcgc aggccacggc gatagattgc
      361 ggggagatca tcttgccagt tacgccgccg ctggtatttg ctgccaccag cagagtgtca
      421 gagacgttga tttgctgcgc cgtggtcgat tgcagtgaac caaacagggc gttagaggag
      481 gtgtccgagc cggtaaggaa tacgcccagc cagccgagaa acggtgagaa gaacgggaac
      541 atcacgcctg tacctgccag taccagcgcc agcgtggtgg acatgccaga atagttggtg
      601 acgaaggcga acgccagcac catgccaatc gacagtatcg gccacttcaa gctaattagc
      661 gtttcggcaa agacgccaat acctttcttg atcccaacac cgaggatgaa gatagagata
      721 atcgccgcaa taaaaatagc ggtgccgcca gccgagagag ggtcgaattt aaacaccgca
      781 tccattggcg ttggttgggc gacaatgggt gccgctttca acacttgttg atgcaaatga
      841 gggatctgga aattaatcac cagtgaataa aacgcgccgc ccggagcaaa taacgcttta
      901 aacggcttca tggtccagat ggtgaccagc accgttaaga ttaaaaacgg tgaccacgca
      961 cgaatgattt gccccagact atattctgaa ggcacgggac cgccagaaga tggcttattt
     1021 accaccatcg cacctgcgga ttgccccatg ctgattgccg tttcggtatt tttcggccgc
     1081 cagactttaa ggaataaagc gagtgagacg atactcacca gcgccgaagt aatatccggc
     1141 agttccggac caatatagtt agaggtaaag aactgagtga cagcaaagct tcccccagca
     1201 accagcgccg ctggccacgt ctctttcacc cctttccagc cgtccatcat tgctaccagc
     1261 cagaacggca caagaaccga caggaacggt aactggcgtc ccgccattgc gccaatgtgg
     1321 aacggatcga ttcccgttac ctgaccggcg accagaatcg gcacgcccaa cgcaccaaac
     1381 gccactggcg cagtgttggc aatcagacac aaccccgccg cgtataacgg tttgaagccc
     1441 aggcccacca gcagcgcacc ggtaatcgcc accggagcac caaagccagc cgctccttcc
     1501 agcaacgcac caaaggagaa accaatcagt aacacctgca aacgctgatc gtcggtgatg
     1561 gagataaccg agctgcggat aatatcgaac tgcccgctgg caacggttaa tttatacagg
     1621 aacaccgccg cgacaataat ccacgcaatt ggccataatc cataaataaa gccatagccc
     1681 gcagcagcaa atgccatatc aatcggcatt ttaaaggcga atattgcaat caggatagat
     1741 aatataaggg ttattgctcc agcgacatgt cctttcagac gtaataccgc gagtgcaacg
     1801 aagaagaata ttatcgggat cagggcgacc agagcggata gccccagtcc tcccatcggc
     1861 atatacattt gggtccaggt aaccatattg ttcagtctct tattatcttt atatgcttga
     1921 tatacttaag gttgtaataa gcaaaagagg actgaactgt aaaatatagg cgttatactt
     1981 tacagcaaca gtacgccgct aacgcaattg ctacctctgg cataacaagt atatcgggta
     2041 agggtttctg ttctgcacac gcaggcgcag agtatcgtta agatgtccat attgttgttt
     2101 taatcccgct agtgatgcgc tacgggtatt taatattgtt aaaccctgat aatcgctccg
     2161 gttatttccg ggataaatgt actaccgcag ttactatcat agccccgaca ataaaacttg
     2221 ccggggcttt tttgacgcta ttaatgactt tctttttcgc gtaaacgcca ggcgtgtaat
     2281 aacggttcgg tataaccgtt tggctgtttc acgccgagga agattaaatc gctggcagct
     2341 ttaaaagcac acgagttagc gaaattcccc gccatcggac gatacgccgg atcgccagcg
     2401 ttttgctgat ccaccacttt cgccatattc tccagcgacg cctgcacctg ttctttggtc
     2461 agaataccgt gacgtaacca gttggcgata tgctggctgg agatacgcag cgttgcgcgg
     2521 tcttccatca gcgccacgtt gtgaatatcc ggcacttttg aacaaccaat cccctgctcc
     2581 acccagcgca ccacataccc cagaatcccc tgcacgttgt tatccagctc ttgttggatc
     2641 tcttccaccg accagttagc gttttcagca accggaatag tcagcagatc gtccagcagc
     2701 ggttcaaatt cagcattgaa ctcggtctgg gcaatgttag cttgcacgct ctgtacgttg
     2761 gtttggtggt agtgcagcgc atggagcgta gcggcagttg gcgacggaac ccaggccgtg
     2821 tttgccccgg cacgcagctg gtcgcctttc tggctgaaca tgtctgccat caggtccggc
     2881 attgcccaca tgcctttgcc gatttgcgct ttaccgcgca gcccacagaa cagaccggaa
     2941 agcacgttat tacgttcgta ggctttgatc cacggcgtcg atttcatctg gtttttacgc
     3001 agcattgggc cagcttccat caccgaatgc atctcatcgc cggtacggtc gaggaaaccg
     3061 gtattgatga acgccacgcg gttgcgcgcc tgagcaatac agctacgcaa gttcagcgag
     3121 gttcggcgtt cttcatccat aatgcccatt ttcagggtat tcggtgccat accgagcatt
     3181 gtctcaacgc gggtaaacag tttgttggca aacgccactt cctgcggacc gtgcattttc
     3241 ggtttcacaa tatagacgct gccagtgcgc gagtttttct gcacttttaa atcataaagg
     3301 gcaatcgcgc ctgtcatgac gccatcaaga atgccttccg ggatttcatt gccttcgctg
     3361 tcccaaatca caggaatggt catcaaatgc cccacgttgc ggataaacag caacgagcgt
     3421 ccgtgcagag aaatttcaga gccatcggcg gcggtgtaat gacgatcgtc attcagttta
     3481 cgcacgattt gccgaccgtt tttttccatt ttctcttgca gagtcccctg catcaggccc
     3541 agcagattgc ggtacagcag gattttatct tccgcatcaa ccgccgcgac cgaatcttcg
     3601 cagtcgagaa tggtactgat ggcagcttcg acgataacat cgttgatgtg cgccggatcg
     3661 tctttgccaa tccgcccgtt ggcatcgatt tgcagctcaa tatgcaggcc gttatttttc
     3721 agcaaaatgc aggtcggcgc agcggtatcg ccacgataac cgacaaactg tgccggggta
     3781 cgtaacgtgg tttctttaac atttttcaac tggatgcgta attgtttgtc aaccacctta
     3841 aacgccacca catcctgata gctgcctttt tccagcggta gtgattcatc gaggaaacgc
     3901 cgaacccagg cgataacctg cgcaccgcgc tgcggatcgt agccgctgac catcgcccct
     3961 tcctgcggga tgatgtcgct gccgtataac gcatcgtaca gtgagcccca gcgcgcgttc
     4021 gccgcgttca gcgcgtagcg ggcattcatc gccggaacca ccagttgtgg ccccgcctgg
     4081 ctggtgattt cgctgtcaat gcccgtggtt tccaccgtca cgcgctccag ttgcggcacc
     4141 aggtagccca gttcacgcag gaaagattta taggccgctt tatcttttac cggccccgga
     4201 ttgctgcgat gccactcatc aagcgccgcc tgaatgcgat cgcgttctgc cagcaactga
     4261 cgattttctg gtgccaaatc gtgaacgatt tcatcaaaat tgcgccagaa cgccgcagcg
     4321 tccagccctg ttcccggtaa aacttcttca tccacaaaac gtttaaaatt ggcgtcaatg
     4381 cgtaaacggc cctgggttat ggtttgactc attgtttatc tcctcgtttt cgcttatttc
     4441 gccaacaccg ctgctgccgc tttcgcgacc tgcgcatcct gtgctccggt taaaccagaa
     4501 acgcccacgg caccaataat ttgcccatcc acaacaactg gtacgccgcc ttccagcgac
     4561 gtcagtaacg gcgcagtcac gaacgcggta cgtccgttgt tcaccatctc ttcatagccc
     4621 ttagtttcac gacgccccag cgcggcggta cgcgctttct cctgggagat gtaagccgca
     4681 atcggcgcgc aatcgtccat gcgacttaac gccagcagat gaccgccgtc atcggcaaca
     4741 gcaatggaaa ccgaccagtt atttttctgc gcctcttcct gacctgcggc aataattgca
     4801 ctcgccattt gctggctaag aatgacttta gttttcattt tgttattcct tttcaagggc
     4861 ttgttctaca atttcaatcc agtgacgcac agaggtacga ccggcgctcg ccagatgcgt
     4921 ctggcaacca atgttggcgg tgacgatcat ttccggtttg ccgctttcca gcgcattcat
     4981 tttgttatcc cgcagctggc gtgccagatc gggatgcgtt aacgcatatg ttcccgctga
     5041 accgcagcac agatggctgt cgggaacgtc cgttaaggta aatccaagac gaagtaacac
     5101 tttttccact tcgccgttca gcttttgcgc atgttgcagg gtacacgggc agtggaaggc
     5161 cagtttttta tcgccgcgaa ttgccagttt ttccagcggt tcctcgcgca gaagttcgac
     5221 taaatcgacc gccagttcac tgacttgacg cgctttatcg gcatataacg catcgttttt
     5281 cagcatctgc ccatactctt tgacgaacgc gccgcaaccg ctggcggttt gcaaaattgc
     5341 ctcggcacct gcttcaatcg tgggccacca ggcatcaata ttatttcgcg cccgtgccag
     5401 ccctttctcc tgcgcattaa gatgatagtc caccgcgcca caacagcctg cttcgttagc
     5461 tgacatgacg ctgatcccca gacgatccag cactcgcgca gttgccgcgt tggtgttggg
     5521 cgaaagcgta ggctgggcgc agccttccag cattaaaacc cgacgcttat ggcgcagcgg
     5581 cggacgcggt ttagctttca cagtttcagc aggcagtttt gctctgacct gttccggtaa
     5641 aaacggtcgc agcaccagcc ctacctgcgt cagcgcacgg aagaccgccg gacgcggcac
     5701 taactggcgc aatccttcgc gcagtattcg ctccggcagt gggcgtttca ctttctgctc
     5761 gacaatatca cgcccgatat ccagcaaatt gtgatagcgc acaccagaag gacaggtggt
     5821 ttcacaattg cggcaagtga ggcagcgatc gagatgctcc tgtgttttaa gcgtgacttc
     5881 gttgccttcc agcacctgtt taatcagata gatgcgcccg cgcggcccgt ccagttcatc
     5941 gcccagaagc tgataggttg ggcaggttgc ggtacaaaat ccgcagtgaa cacaggcgcg
     6001 caggatgctg tcggcttcca gcgcgcgcgc gttctgccgc atctcttcag ttaattgggt
     6061 ttgcatagcc tgctcctcag agttccgcgt acatgcgacc ggggttaaac acgccgcaag
     6121 ggtcgagctg ctgtttaagc tgctggtgat agcggaataa aggagccgat agcggggcaa
     6181 agccaccatc tccggcacta aagcgggtcg catgaccgcc agcgttgcgg gcgatgcgat
     6241 ggatttgatt gtcctcggct gtcgatttca gccagcgtaa cgcaccgccc cagtcgatca
     6301 gttgctcgcc gggtaaatcc atcatcggcg catcgctggg taatgaaatg cgccataagg
     6361 tacctggtaa cgagaagaac ggcagttgtt gttcacgcaa ttgctgccag aactgacccg
     6421 caacctcttc gccccccagc agttcacgcg ccgcttttac cgatccttcg ccgccctcaa
     6481 ggcggatcca caacgcattg tcgaagtaac ataagccact aataggtagc ggctggagtt
     6541 gccactgggc gatttcgttc atggcttctt gcaggctgat ttcccgacgc aggctcaggg
     6601 aggcgcgcgg tcgcggtaac actttcatcg agatttcagt gagcacgcca agacaaccgt
     6661 agcttccggc cattaaccgt gagagatcgt atccggcaac gtttttcatc acttcgccac
     6721 caaaacgcag atgttttcca gcgccggtaa tgatgcgcgt gccgaggaca aaatcgcgga
     6781 ccgaaccgct ccacgggcga cgcggccccg ccagcccgca ggcgaccatc ccgccccagg
     6841 tggcttcttc accataatgc ggcggctcgc agggaagcat ttgcccggcg ctctccagcg
     6901 ccgcttcaat cgccaccagc ggcgttccgg cacgcgcggt tatcaccagc tcggtcgggt
     6961 cgtaattaac aatgccgcga tgacaacgca catccagcgt ttgcccggtg acagggcgac
     7021 ctaaaaaggc tttgctattg ctgccctgaa tcaccagcgg cgttttatcg ctaattgcct
     7081 gattcacctg ctccagcagc gcctgactgt aatcacactc gcgtagcatc agaaacgctc
     7141 cagttcaggg aaaggtaaat gaccgtgatg cacatgcatg gcaccaaatt cagcacagcg
     7201 gtgtagcgtg ggaatgtttt tcccagggtt cagcaaacca tcggggtcaa acgccgcctt
     7261 gaccgcatgg aaggtcgtga tttcatcgct gttgaactgg gcgcacattt gattgatttt
     7321 ttctcgcccg atgccatgtt ccccactgat gctgccgcca acttcaacgc agagttcgag
     7381 gatcttccca cccagctctt ccgcgcgggc aaattcaccg ggttcgttgg catcgaaaag
     7441 gattaacggg tgcatgttgc catctccggc atgaaagacg ttggcaacac gtaaatcata
     7501 ttgctgcgat aaacgggcaa tgccttccag tacgccaggc agggcgcgac gcgggatggt
     7561 gccatccatg cagtagtaat ccggggagat acgtcctacc gccgggaacg catttttgcg
     7621 accggcccag aaacgtacgc gctctgcttc gtcctgtgcc agacggacgt cagtcgcgcc
     7681 cgctttcaac aagatgtcgt taacccgctc gcagtcttcc tgtacgtcag actccacgcc
     7741 gtccagctcg cataacaaaa ttgcttcggc gtcgacggga taaccggcat gaataaaatc
     7801 ttccgccgcg cggatcgaca ggttatccat catctccagc ccgccgggga taatgccatt
     7861 ggcgatgatg tcaccaactg caagtccggc tttttctacc gagtcaaagc tggctaacag
     7921 aacccgcgcc acgggcggct tcggcagcag ttttaccgtc acttcggtgg tcacgccgag
     7981 cataccttcc gatccggtga acagcgccag caggtcaaaa ccaggtgaat ccagcgcgtc
     8041 cgatcccagc gtcagcgcct cgccgtccag cgtttgcact tcaattttca gcaggttatg
     8101 tacggtcaga ccatatttca ggcagtggac gccgccggca ttttctgcca cgttgccgcc
     8161 aatggaacag gcgatttgtg aggaagggtc cggtgcgtag tagagattat gcggtgcaac
     8221 ggcctgggag atcgccaggt tacgcacgcc tggctgcacg cgcgcgcggc gaccaacggg
     8281 gttaatgtcg aggatctctt taaagcgcgc catcaccaac aacacacctt tttccagcgg
     8341 cagcgcgcca ccagaaagcc cggtgcctgc accacgggtc accaccggta cacgcaggcg
     8401 atggcagaca gccagaatcg ctgtcacctg ttccatttgc ttaggcagaa caaccagtaa
     8461 tggacgcgtg cgatacgcgc tcaacccgtc acactcgtaa ggaatgatct cctcatcggt
     8521 atgcaggatc tcaagtccag ggacatgctc acgcagtgcc atcagtaccg atgtgcggtc
     8581 gacatcgggt aaagcgccat caagacgctc ttcgtacaag atgctcatga gtaggcttcg
     8641 ctttgttgtg tggcagctga tttttgcgcg ctgcttctgt gaacagttat taagcgggct
     8701 tttcgttttc gtctatctct ttagctaccg gtcagaccat tttttttcca gctctgtgac
     8761 cttgccttgg ttaactcaat gttaaattga tgtaacataa tcacttacgt gatgtgcgtg
     8821 ttttgcgagt taagaacaga aaaattggtc ctacctgtgc acgaggtccg ggaatgaaag
     8881 atgaacgtcg ccctatttgc gaagtggttg cagagagtat cgaacggtta attatcgacg
     8941 gcgtactgaa ggtcggtcag ccacttccct cggaacgtcg actgtgtgaa aagctcggct
     9001 tctcacgctc cgcactgcgt gaagggctga ccgtgctgcg cgggcgcggg attattgaaa
     9061 cggcgcaggg tcgcgattct cgtgtcgcac ggcttaatcg ggtgcaggac accagcccgc
     9121 tgatccatct gttcagtacg cagccgcgaa cgctgtacga tctgctcgac gttcgcgcat
     9181 tactggaggg cgaatcggca aggctggcgg caacgctggg aacgcaggct gattttgttg
     9241 tgataacccg ctgttatgaa aaaatgctcg ccgccagtga gaacaacaaa gagatttcgc
     9301 tgatcgaaca tgcgcagttg gatcacgctt tccatctcgc catttgtcag gcttctcaca
     9361 atcaggtgct ggtgtttacg ctgcaatcat tgaccgatct gatgtttaat tcagtgtttg
     9421 ccagcgtaaa taatctctac catcgaccac agcaaaaaaa gcagatcgat cgccagcatg
     9481 cgaggatcta caacgcggtg ttgcagcggc tgccgcacgt cgctcagcgc gcagcacgcg
     9541 atcatgtgcg gaccgtgaaa aagaatctcc acgatatcga gctggaaggc caccatttga
     9601 ttcgctcggc ggtgccgctg gagatgaacc tgagttagct ggtattaaat ctgcttttca
     9661 tacaatcggt aacgcttgta cggctccgcc ccaatgcgtt ccagcatgtt attcatgcct
     9721 gtattggttt cgaggatcca tgacatctcc agcgcatcga tcttccggcg ggcaaacgga
     9781 tcgcgtaagg cttcaatcaa taacagcgca atcaccgggc cgatgcggct gaactgatac
     9841 tcgtcgcgca cgcccatcag cggtactcgc gcagttcgca caccgctgac tttcaaacgc
     9901 cacagcaatt ttgcccagcc gaagggaaag agcgatccgt tcagatcggc aatcgcctcg
     9961 ttgatgttcg gcaagccgac aataaacgcg cagggtgcag aatcaatctc agcgatgtag
    10021 atcatatcgt ccggcaccag atatttaagt tgatcgccca tggtcgcgaa ttcatgttcg
    10081 gtaaacggca caaatcccca gttgtgctgc cagccagagt tgaaaatctc acgcaggatc
    10141 tgcatctctt cggcaaaccg ctgacgattg atgcagcgaa tggtcacctt tttgcgcacc
    10201 tgatccatca gtttttttag cgccggagag aaagtgagat cggttcgctg catccaccac
    10261 gccagtaaat caatgccttt gtgataaccc agttgttcaa tatgcgcggc ataccacggt
    10321 ttgccgtgtg gcatcatcgc acagggtggt gtgtcaaaac cttcaatcag taatccgctt
    10381 tcctgattga tattcaggct gaaaggcccg ctgatcttac ttgcaccttg tgacttcaac
    10441 cacgcttccg ctgcgccaaa caacgcggca aaaacctgcg gatcatcaat ggcgtcaatc
    10501 atgccgaaat gaccggtatc ttcaccgtaa cgctcacagt gcaaggtatc tatttgcgcg
    10561 gtaatacgcc caactatctg ccccgctttt tttgccaccc acgcctgcca gatgatatgg
    10621 tccgtccccg gatttttcgc agacaaatgc tcgttgcgtt caatgaataa agggggtatc
    10681 cagtttggat cgtcgggata aagtgatgac ggaaaagcga taaatgcctt aaggtcattt
    10741 ttattaatta ctttttcaat tttaatcatc agcagatcgc attccatatc tctgtggata
    10801 acaaataatt aacggtgaaa aacaggttgc aacagcaacg aacgacacct taataatcaa
    10861 cccaatgaat acaaacaaag aataaatagt
//
LOCUS       DBANUU010000144        10878 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000144 DBANUU010000000
VERSION     DBANUU010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.145"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            58..819
                     /gene="adiY"
                     /locus_tag="ACIE68_003859"
     CDS             58..819
                     /gene="adiY"
                     /locus_tag="ACIE68_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFX5910105.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            956..2293
                     /gene="adiC"
                     /locus_tag="ACIE68_003860"
     CDS             956..2293
                     /gene="adiC"
                     /locus_tag="ACIE68_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFX5910106.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2397..4040
                     /gene="eptA"
                     /locus_tag="ACIE68_003861"
     CDS             2397..4040
                     /gene="eptA"
                     /locus_tag="ACIE68_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFX5910107.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4037..4705
                     /gene="basR"
                     /locus_tag="ACIE68_003862"
     CDS             4037..4705
                     /gene="basR"
                     /locus_tag="ACIE68_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFX5910108.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4706..5806
                     /gene="pmrB"
                     /locus_tag="ACIE68_003863"
     CDS             4706..5806
                     /gene="pmrB"
                     /locus_tag="ACIE68_003863"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFX5910109.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5782..5871)
                     /gene="pmrR"
                     /locus_tag="ACIE68_003864"
     CDS             complement(5782..5871)
                     /gene="pmrR"
                     /locus_tag="ACIE68_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFX5910110.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5983..7485)
                     /gene="proP"
                     /locus_tag="ACIE68_003865"
     CDS             complement(5983..7485)
                     /gene="proP"
                     /locus_tag="ACIE68_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFX5910111.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7749..8627)
                     /gene="rdcB"
                     /locus_tag="ACIE68_003866"
     CDS             complement(7749..8627)
                     /gene="rdcB"
                     /locus_tag="ACIE68_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFX5910112.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            complement(8624..10852)
                     /gene="crfC"
                     /locus_tag="ACIE68_003867"
     CDS             complement(8624..10852)
                     /gene="crfC"
                     /locus_tag="ACIE68_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFX5910113.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDRLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHDDLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQARGYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEVKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2727 a         2845 c         2683 g         2623 t
ORIGIN      
        1 cggtatcgct ttgctttagc gagaactggt cttttatttg cactcaggag tacatgtatg
       61 aggatttgca gcgaccaacc ttgtattgtt ttattgactg aaaaagatgt ctggataagg
      121 gtgaatggga aagaacctat tagccttaaa gctaaccata tggcgttatt aaattgtgaa
      181 aataatatta tcgacgtctc ctctcttaac aacactttgg ttgctcatat tagtcacgac
      241 atcatcaaag attacctccg gtttctgaat aaagatctct cgcaaatacc agtatggcaa
      301 cgtagcgcta cgcccatact caccctgcca tgcctgacgc cagacgtctt tcgcgttgcc
      361 gcgcaacaca gcatgatgcc cgcagaaact gagtcagaaa aggaacgaac acgcgcatta
      421 ttattcacgg tgctatcccg ttttctcgac agtaaaaaat tcctttcatt aatgatgtat
      481 atgttacgta attgtgtaag tgacagcgtt tatcaaatta ttgaaagcga tattcacaaa
      541 gactggaatc ttagtatggt agccagttgt ttatgtctta gcccaagtct gttaaagaaa
      601 aagctgaaaa gcgaaaacac cagttatagc caaataatca ccacctgccg catgcgttat
      661 gccgtaaatg aattaatgat ggacggtaaa aatatctctc aggtatcaca gtcctgcggc
      721 tacaacagta cgtcgtactt tatttctgtc tttaaagact tctacggtat gacgccgctg
      781 cattatgtta gtcagcacag agaacgcact gtcgcctgat ttttaacctt aacgaagagc
      841 tatattaata acggcatcag cgataacccg gtcgataata attcaactat cgaatgcagg
      901 cgtatgatat gacgtaattt attgtcacga agctcgcctt cgcaggagtt taattatgtc
      961 ttcggatgct gatgctcaca aagtgggctt aatccccgtc accctgatgg tgtcggggaa
     1021 tattatgggg tcaggtgttt ttctgttacc tgcaaacctg gcctcgactg gcgggattgc
     1081 catttatgga tggttggtga cgattatcgg tgcgctgggg ctctcgatgg tatacgccaa
     1141 aatgtcgttc ctcgatccaa gtcctggtgg ttcttacgct tacgcccgcc gctgctttgg
     1201 cccgtttctc ggttatcaaa ccaacgtcct ctactggctg gcctgctgga tcggcaatat
     1261 cgccatggtg gtcattggcg taggatattt aagttacttc ttcccgattc tgaaagatcc
     1321 attggtatta accatcacct gcgtcgtggt gctgtggatc ttcgtcctgc tgaacattgt
     1381 cggtccgaaa atgatcaccc gtgtgcaggc agttgccacc gtgctggcgc tgatccccat
     1441 cgtcgggatt gccgtatttg gctggttctg gttccgtggt gaaacctata tggcggcatg
     1501 gaacgtcagc ggcctgggca ccttcggtgc aattcaaagt acccttaacg ttacgctgtg
     1561 gtcgttcatc ggtgtggaaa gtgcctccgt tgccgcaggt gtggtgaaaa acccgaaacg
     1621 caatgtccct atcgccacca ttggtggggt attgattgcc gccgtttgct atgtactttc
     1681 taccaccgcg attatgggga tgatccctaa tgccgcactg cgcgtttctg cttcgccatt
     1741 cggtgatgcc gcacggatgg cgttgggtga caccgccggg gccattgttt ccttctgcgc
     1801 agctgcgggt tgcttaggtt cactgggcgg ctggacgttg ctggcgggtc aaacggcgaa
     1861 agccgctgcc gatgacggac tgttcccacc aatttttgcc cgtgtaaata aagcgggtac
     1921 gccagtggcg gggctgatta tcgtcggtat tttgatgacc atcttccagc tcagcagcat
     1981 ttcaccaaac gcgaccaaag agttcggtct ggtttcttcc gtctcggtca tctttacact
     2041 ggtgccatat ctttacacct gtgcggcgtt actgctgctc ggacacggtc actttggtaa
     2101 agcacgcccg gcatatctgg cagttactac cattgccttc ctctactgca tctgggccgt
     2161 ggtggggtcc ggagcgaaag aggttatgtg gtcatttgtc accctgatgg tcatcaccgc
     2221 catgtatgcg ctgaattaca accggctaca taaaaacccg tatcccttag atgcaccaat
     2281 aagcaaagat taattcccct taatccagca aacataaaag ccaaccttaa gaacttaagg
     2341 ttggcttaat tttgctttgc gagcatatgc gcactttgtt cgatggaaac accgtgatgt
     2401 tgaagcgcct actaaaaaga ccctctttga atttactcgc ctggctattg ttggccgctt
     2461 tttatatctc tatctgcctg aatattgcct tttttaaaca ggtgttgcag gcgctgccgc
     2521 tggattcgct gcataacgta ctggttttct tgtcgatgcc ggtcgtcgct ttcagcgtga
     2581 ttaatattgt cctgacacta agctctttct tatggcttaa tcgaccactg gcctgcctgt
     2641 ttattctggt tggcgcggct gcacaatatt tcataatgac ttacggcatc gtcatcgacc
     2701 gctcgatgat tgccaatatt attgatacca ctccggcaga aagttatgcg ctgatgacac
     2761 cgcaaatgtt attaacgctg ggattcagcg gcgtgcttgc tgcgctgatt gcctgctgga
     2821 taaaaatcaa acctgccacc tcgcgtctgc gcagtgttct tttccgtgga gccaatattc
     2881 tggtttctgt actactgatt ttgctggtcg ccgcactgtt ttataaagac tacgcctcgt
     2941 tgttccgcaa taacaaagag ctggtgaaat ccttaagccc ctctaacagc attgttgcca
     3001 gctggtcatg gtactcccat cagcgactgg caaatctacc gctggtgcga attggtgaag
     3061 acgcccaccg caacccgtta atgcagaacg aaaaacgtaa aaatttgacc atcctgattg
     3121 tcggcgaaac ctcgcgggcg gagaacttct ccctcaacgg ctacccgcgt gaaactaacc
     3181 cgcggctggc gaaagataac gtggtctatt tccctaatac cgcatcttgc ggcacggcaa
     3241 cggcagtttc agtaccgtgc atgttctcgg atatgccgcg tgagcattac aaagaagagc
     3301 tggcacagca ccaggaaggc gtgctggata tcattcagcg agcgggcatc aacgtgctgt
     3361 ggaatgacaa cgatggcggc tgtaaaggtg cctgcgaccg cgtgcctcac cagaacgtca
     3421 ccgcgctgaa tctgcctggt cagtgcatca acggcgaatg ctatgacgaa gtgctgttcc
     3481 acgggctgga agagtacatc aataacctgc agggtgatgg cgtgattgtc ttacacacca
     3541 tcggcagcca cggtccgacc tattacaacc gctatccgcc gcagttcagg aaatttaccc
     3601 caacctgcga caccaatgag atccagacct gtagcaaaga gcaactggtg aacacttacg
     3661 acaacacgct ggtttacgtc gactatattg ttgataaagc gattaatctg ctgaaagaac
     3721 atcaggataa atttaccacc agcctggttt atctttctga ccacggtgaa tcgttaggtg
     3781 aaaatggcat ctatctgcac ggtctgcctt atgccatcgc cccggatagc caaaaacagg
     3841 tgccaatgct gctgtggctg tcggaggatt atcaaaaacg gtatcaggtt gaccagaact
     3901 gcctgcaaaa acaggcgcaa acgcaacact attcacaaga caatttattc tccacgctat
     3961 tgggattaac tggcgttgag acgaagtatt accaggctgc ggatgatatt ctgcaaactt
     4021 gcaggagagt gagtgaatga aaattctgat tgttgaagac gatacgctgt tattgcaggg
     4081 attgattctg gcggcgcaaa ccgaaggcta cgcgtgcgat ggcgtgacaa ccgcgcggat
     4141 ggcggaacaa agccttgagg ccggtcatta cagcctggtg gtactggatt tagggttacc
     4201 cgacgaagat ggactgcatt ttctcgcccg tatccggcag aaaaaataca ccctgccagt
     4261 actgatcctc accgctcgcg atacgctgac cgacaaaatc gccgggctgg atgtcggtgc
     4321 cgatgactat ctggtgaagc cttttgcgct ggaagagtta cacgcccgta tccgcgccct
     4381 gctacgacgc cataataatc agggcgaaag tgagctgatt gttggcaatc tgacgctgaa
     4441 tatgggtcgc cgtcaggtat ggatgggcgg tgaagagttg attctgacgc ccaaagaata
     4501 tgctctgctg tcacggttaa tgctcaaagc aggcagtccg gtacatcggg aaattctcta
     4561 caacgacatc tataactggg acaatgaacc ctcgaccaac accctggaag tgcatatcca
     4621 caatctgcgc gacaaagtgg gcaaagcccg tatccgcacc gtgcgcggct ttggctatat
     4681 gctggtcgcg aatgaggaaa actaattgaa tctgatgcat tttctgcgcc gaccaatatc
     4741 gctgcgccaa cggctgatat tgaccatcgg ggccattttg ttggtgtttg agctgatcag
     4801 cgtcttctgg ctatggcatg aaagtaccga gcagattcag ctgtttgagc aggcgctacg
     4861 cgacaatcgc aacaacgatc gccacatcat gcgtgagatc cgcgaggcgg tcgccagcct
     4921 gattgtcccc ggcgtcttta tggtcagcct gacgctattt atctgctatc aggcggtgcg
     4981 ccgcatcacc cgcccgctgg cggagctgca aaaagagctg gaagcgcgca ctgccgacaa
     5041 cttaacgccc attgccattc acagcgccac cctcgaaatc gaagcggtgg tttcggcgtt
     5101 aaacgatctg gtcagtcgcc tgaccagcac gctggataac gaaaggttgt ttaccgctga
     5161 cgtcgcgcac gaactgcgaa cgccactggc gggggtgcgt ttgcatctgg aactgctggc
     5221 gaaaacgcat cacattgatg tagcaccgtt agtggcacga cttgatcaga tgatggagag
     5281 cgtctcccag ttgctgcaac tggcgcgtgc cggacagtca ttttcttccg gtaattatca
     5341 acatgtaaaa ctgctggaag atgtcattct cccctcgtat gacgaactca gtaccatgct
     5401 cgaccagcga cagcaaaccc tgctattgcc agagagcgcc gccgacatca ccgttcaggg
     5461 tgatgcaacc ctgctgcgga tgttattgcg aaacctggta gaaaacgccc atcgttacag
     5521 cccgcaaggc agcaacatta tgattaagct gcaagaagat ggcggagcgg tcatggcagt
     5581 tgaagatgaa ggaccaggta ttgatgagag taaatgcggg gagttgagta aagcgtttgt
     5641 acgtatggac agccgttatg gcgggattgg tctggggtta agtattgtca gccgcattac
     5701 acagttgcat cacgggcagt ttttcctgca aaaccggcaa gagacttccg gcacgcgggc
     5761 ctgggtacgg ctgaagaaag atcagaacgt ggcaaaccag atataaagaa agctgctgac
     5821 caccagcacg ctgaacacgg tagttaaact ttcataaaca cggtttttca ttgcactctc
     5881 ctccgttaac ctggaggaga gtatgcgcgt cagagattaa gtgaacctta agagttcaat
     5941 tggcctgata agacagcgtc acatcaggcc atccgtttca gcttattcat caattcgcgg
     6001 atgttgctgc accaggcggg tacgtttcgc ctgcaaatcg gcaatctcgt ggtcaatatt
     6061 atcgattttc tgctcgatat tatcgtaatg ctcgacgaga atttccttcg cttcctgtat
     6121 atctgacgcc gccggtgtcg cacctttcaa cggacgattt gccgtctctt tcatggttac
     6181 gccggtgatt aaaccaatca ccgccactac catcaggtaa taggcaggca tcatcagatt
     6241 ctgcgagctt tcgaccagcc aggccgccag tgttggcgtc agaccggcaa ccagcaccga
     6301 aatattaaat gccgccgcca gcgcgctgta acggatatgc gtcgggaaca tcgctggcaa
     6361 ggtagaagcc ataacgcccg taaagcagtt aaggatcacc gccagcatca gtaacccggc
     6421 aaaaatcagg ccgatgacgt tactgttaat cagaataaac gccgggatcg ccaacacaaa
     6481 cagggcaaca ctaccaagta gcacaaacgg acgacggcca aaacggtcac tcagcaagcc
     6541 catcaccggc tggacaaaca gcataccgat cataatggcg ataataatca gcaccccgtg
     6601 gtcttcggag taatgcaggt tatgcgacaa ataactcggc atataggtca gcaacatgta
     6661 gtaagtcacg ttggtggcaa ttaccagacc aatacatgtc aacaggctgc gccagtgttt
     6721 ggtggcaatc tctttaaacg agactttcgg gccatcctgc aaaccttcgc ggtcgccctg
     6781 ttccagttta tcgacatgct gctggaacgc cggagtttct tccagcgcat ggcgcaggta
     6841 aagcccgata atccctaacg gcagagcaat aaagaacgga atacgccagc cccagtcgag
     6901 gaagttcgct tcgccgacaa tggtcgaaat taacaccact acgccagcac ccagcacaaa
     6961 cccggcaata gaaccaaagt ccagccagct gcccataaag ccacgtttac ggtcagggga
     7021 gtattccgca acaaatatcg acgccccggt atattcaccg ccgaccgaga aaccttgtgc
     7081 catcttacag atcaacagca gaatcggtgc ccaaatacca atcgtgtcgt aggacggtat
     7141 taagccaata cagaacgtac tgatcgacat aatcacaata gtgatagcga ggatcttctg
     7201 gcgaccatat ttatcgccca acataccaaa gaagagtccg ccaagcggtc gaatcagaaa
     7261 gggaacggag aacgtggcaa gtgcagcaac catctgcacg ctggggtcag cccccgggaa
     7321 aaaaacttta cctaatgcgt aagcaacaaa accataaaca ccaaaatcaa accattccat
     7381 tgcattaccc agtgatgctg cggtaatggc tttacgcagt ttaccgtcat caataatggt
     7441 gacatcacga agggtaatcg gttttacttt tttccttttc agcatagctt tcctcgcaga
     7501 gttggcccat ttccgcaaac gccagaaaaa tcagcgcgac ggccgcaaaa ggggagcggg
     7561 aattgcagga tgcaatctct gtaacaagcg tagcgggttt gcttacaccc tccggttaag
     7621 gaatgtacaa tcaaaccagt tgacgcctga attgggcagt taatgacctt tttaccctac
     7681 cagtgtttaa atttgtgatc aacttcacac atggatttcg tttaggactc attgatgtaa
     7741 ctgattattt acgccaaatt tacagagaag atttcctcga ccattgcccc cgccagacgc
     7801 tgcgctgaac agagtcgtgg catccccagc gcgacagttt gccagcgttg ggtcactgac
     7861 cagcaaacgg gttgtttatc tgccgtggtc cactttccca gccaacttaa aaaatcagca
     7921 tgatccccgc ccgtcggaac ggtcgaagag gcaaaccact gatggtaata gtgacgcgtg
     7981 gtcggggccg cattgatact ctctgccaga tgttgtgtcg ctttcacgcg caaatcaccc
     8041 agcaacaacg ccaccgcttg cgggcttccc agacgacagg catcgccaaa tgccccccaa
     8101 cgcaactctt cgagaacgat aaaacagcgc ccaggcaacg accaggcgtt atacgcccca
     8161 gcacgccagc gggtaaaaat ttgctcgctc tgttcacccg cctgcaccag taggcctcgt
     8221 tgcgttaatg ccttttcctt ccagcgacaa cgggctgaaa accactcata caaggcggca
     8281 atttgctggg ggagctccgg gaaagcagag tgctggcggt gatccatttt ttgcagcagc
     8341 gccagatgct gcgcgatctg cgtcagtgcc agatgaccgg gatccagcat cgacgccagt
     8401 ttttccacca atgtcagacg caggctaagc ggcgcattta acatgccgcc gttcgtccat
     8461 gtctgaagct gcacgcgcgc aagaagttcc tgcatcagtc gttcacgaaa ctgctgctga
     8521 tgggcttgtg gaaggcgggc atcatcgccc tgcgccagat ccactaaaaa gcggggataa
     8581 accgactcca gcacgcgacc ggggccgtca agtaacgtct tggtcaatat cgttctgccg
     8641 tgaaaagtgt ttgaatatca tcgcgtaaca gctgggcgtc ggtgtaaatc cagccgtgag
     8701 tcatcacagt ctgctgcaat tgctgctgca tcagcctgac caccgattca ttttgttgac
     8761 gcagagccag actttcgcgt aaacgcgtct gtaattccgt caaacatgaa gcgaactcag
     8821 cgaaaaaagt attcatgcct gccgtaacag attcatcgac ctgctctgcc agaactttag
     8881 ccatttgttg gcaataaaga tcgacttctg cgcttaaagc tcgttgcaac acactgtaat
     8941 caaccgtttc tgtcggggat ttctcatttc cccgtcccca gtcgggctga ttcaaccagc
     9001 gcgaaaaagt ctcacgcaca acgcctaaac gcgtgctctg ctcgtccgtt gcttcctggc
     9061 gcgaaatgac ttcactgaac agctggcgag tgttgaagtg gggaactacg ccgtgaaaaa
     9121 caggaaaatg aaacccagga cgaaaccctg actcgctcaa ttccattttg acttgttgct
     9181 caatgggccg aataacatcg gttaacaccc ggcaaagggt ggattcaagc tcggcaaaac
     9241 gcagcgtaaa gtcgcgactg atggtgttct gcgccgtctg taacagtgtc tcacagcagg
     9301 tacgaatctc gcttaacggc tccgaatcat cctgaaacaa ggcggctaac tgcgcattca
     9361 gcgcatcttg ctgttgacgc agaaagtggt tggcggaggt cagggccagc tcgatttcat
     9421 gtttaacctc gccgctcacc tgcgcctgat tgagttgcaa tagctgcaaa ctttcttcga
     9481 cctgatggat attttgccgc aattgttcac aagcgacgtt taacccgtgc gcacgaaaat
     9541 ccaggtatcc ccgcgcctgc tgcgcgtaat tcaacagttt atgcgcagca gatcgcagag
     9601 catacaacga ggcgttagcg taagcggcat gaagcaacgc ctgaattggc tgggcgaaca
     9661 gcgaatcttc ccacaactga tcggcagcat gacgaatatg ttcgaggtcc gccagatcgt
     9721 catgacgcca gcgcctgccg agcgcagcat gggcaaaatc ttccacccag cgttgttgct
     9781 ctggcgctgg taacttaccg ttgttggcta actcatggcg cgctcgattc gccaggtagc
     9841 cccacatcga cgacaccgga aatatctgct gtggcgtaat acagcctttc atcagcgtcc
     9901 cggaaatcag tgcccgcacc tggtcggcgt cgtcactgtt acgatcctgt tgatcgaact
     9961 tattgaccag cacatacagc ggcaccgatt gccccaccgc caaaatcgcc tcacggacct
    10021 cttcatcgga gatcgatttc agttgcgtat aatccagcac cgccagtacc gccgaggcgc
    10081 gtgccagctg ctggttaagc attttttgca gatgcggttg cccggcttca tttggcccgg
    10141 gggtatccag taacgtcaac tgaccgggat aactctccag ccccgccaga tggacaaact
    10201 ccacttcaat cacgggaata tgctcaatgg cggcgtaagc agaaaaagga aaatcgacgt
    10261 ccagcgcctt cgccagtcgc actaaatcat tcaaactttt cagacaatga aaaataggct
    10321 gggcacccag ataatatttt tcgaaagcga cgccattttc gatccgctgc ataagcgcac
    10381 gcatatcttt atctatttcc agcacatcgg tcagatgctt aatatcgcaa tcacgcaggc
    10441 gctgttgtaa tttttgaatt aaacggtcga ttggcgcgac atgtgaaaaa tgcagtaccg
    10501 gttctttttg cccgggcgta tggcgaataa gcgtcggcag cgcagtcatt gggcgattac
    10561 gattaggcaa aacctccgta ccaacaatgg cattaatggt ggttgatttc cctgctttca
    10621 tggtaccgac aatcgccagc accatttcca gtcgggaaat tttacgcaac tcattattca
    10681 gcatcgcgtg acgttcggcg atattaggct gactccaggg taaagccagt tgtggcgcat
    10741 cgtctccggg tacagagaga ggcatttttt ccagtaactg caactgttgg cgagaaagct
    10801 gtaacaggcg ttcagcctcc tgacttaact catacagggt ctgtgtgtac atagaaaatt
    10861 cttccttaaa gcaaattt
//
LOCUS       DBANUU010000145        10831 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000145 DBANUU010000000
VERSION     DBANUU010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.146"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            252..656
                     /gene="sdhC"
                     /locus_tag="ACIE68_003868"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             252..656
                     /gene="sdhC"
                     /locus_tag="ACIE68_003868"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFX5910114.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            650..997
                     /gene="sdhD"
                     /locus_tag="ACIE68_003869"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             650..997
                     /gene="sdhD"
                     /locus_tag="ACIE68_003869"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFX5910115.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            997..2763
                     /gene="sdhA"
                     /locus_tag="ACIE68_003870"
     CDS             997..2763
                     /gene="sdhA"
                     /locus_tag="ACIE68_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFX5910116.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2779..3495
                     /gene="sdhB"
                     /locus_tag="ACIE68_003871"
     CDS             2779..3495
                     /gene="sdhB"
                     /locus_tag="ACIE68_003871"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFX5910117.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPMPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3796..6597
                     /gene="sucA"
                     /locus_tag="ACIE68_003872"
     CDS             3796..6597
                     /gene="sucA"
                     /locus_tag="ACIE68_003872"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFX5910118.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6612..7829
                     /gene="odhB"
                     /locus_tag="ACIE68_003873"
     CDS             6612..7829
                     /gene="odhB"
                     /locus_tag="ACIE68_003873"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFX5910119.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            7923..9089
                     /gene="sucC"
                     /locus_tag="ACIE68_003874"
     CDS             7923..9089
                     /gene="sucC"
                     /locus_tag="ACIE68_003874"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFX5910120.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9089..9958
                     /gene="sucD"
                     /locus_tag="ACIE68_003875"
     CDS             9089..9958
                     /gene="sucD"
                     /locus_tag="ACIE68_003875"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFX5910121.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(10062..10784)
                     /gene="mngR"
                     /locus_tag="ACIE68_003876"
     CDS             complement(10062..10784)
                     /gene="mngR"
                     /locus_tag="ACIE68_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFX5910122.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
BASE COUNT         2551 a         2807 c         2998 g         2475 t
ORIGIN      
        1 gtaggttaat tgtaatgatt ttgtgaacag cctatactgc cgccaggtct ccggaacacc
       61 ctgcaatccc gagccaccca gcgttgtaac gtgtcgtttt cgcatctgga agcagtgttt
      121 tgcatgacgc gcagttatag aaaggacgct gtctgacccg caagcagacc ggaggaagga
      181 aatcccgacg tctccaggta acagaaagtt aacctctgtg cccgtagtcc ccagggaata
      241 ataagaacag catgtgggcg ttattcatga taagaaatgt gaaaaaacaa agacctgtta
      301 atctggacct acagaccatc cggttcccca tcacggcgat agcgtccatt ctccatcgcg
      361 tttccggtgt gatcaccttt gttgcagtgg gcatcctgct gtggcttctg ggtaccagcc
      421 tctcttcccc tgaaggtttc gagcaagctt ccgcgattat gggcagcttc ttcgtcaaat
      481 ttatcatgtg gggcatcctt accgctctgg cgtatcacgt cgtcgtaggt attcgccaca
      541 tgatgatgga ttttggctat ctggaagaaa cattcgaagc gggtaaacgc tccgccaaaa
      601 tctcctttgt tattactgtc gtgctttcac ttctcgcagg agtcctcgta tggtaagcaa
      661 cgcctccgca ttaggacgca atggcgtaca tgatttcatc ctcgttcgcg ctaccgctat
      721 cgtcctgacg ctctacatca tttatatggt cggttttttc gctaccagtg gcgagctgac
      781 atatgaagtc tggatcggtt tcttcgcctc tgcgttcacc aaagtgttca ccctgctggc
      841 gctgttttct atcttgatcc atgcctggat cggcatgtgg caggtgttga ccgactacgt
      901 taaaccgctg gccttgcgcc tgatgctgca actggtgatt gtcgttgctc tggtggttta
      961 cgtgatttat ggattcgttg tggtgtgggg tgtgtgatga aattgccagt cagagaattt
     1021 gatgcagttg tgattggtgc cggtggcgca ggtatgcgcg cggcgctgca aatttcccag
     1081 agcggtcaaa cctgtgcgct gctctctaaa gtcttcccga cccgttccca taccgtttct
     1141 gcgcaaggcg gcattaccgt tgcgctgggt aatacccatg aagataactg ggaatggcat
     1201 atgtacgaca ccgtaaaagg ttcggactat atcggtgacc aggacgcgat tgaatatatg
     1261 tgtaaaaccg ggccggaagc gattctggaa ctcgaacaca tgggcctgcc gttctcgcgt
     1321 cttgatgatg gtcgtatcta tcaacgtccg tttggcggtc agtcgaaaaa cttcggcggc
     1381 gagcaggcgg cacgcactgc ggcagcagct gaccgtaccg gtcacgcact gttgcatacc
     1441 ctttatcagc agaacctgaa aaaccacacc actattttct ccgagtggta tgcgctggat
     1501 ctggtgaaaa accaggatgg cgcggtggtg ggttgtactg cactgtgcat cgaaactggt
     1561 gaagtggttt atttcaaagc ccgcgctacc gtgctggcga ctggcggggc agggcgtatt
     1621 tatcagtcca ccactaacgc ccacattaac actggcgacg gcgtcggcat ggctatccgc
     1681 gcaggcgtac cggtgcagga tatggaaatg tggcagttcc acccgaccgg tattgccggt
     1741 gcgggcgtac tggtcaccga aggttgccgt ggtgaaggcg gttatctgct gaacaaacat
     1801 ggcgaacgct ttatggagcg ttatgcgccg aacgccaaag acctggcggg ccgtgatgtg
     1861 gtggcgcgtt ccatcatgat cgaaatccgt gaaggtcgcg gctgtgatgg tccgtggggg
     1921 ccacacgcga aactgaaact cgaccacctg ggtaaagaag ttctcgaatc ccgcctgccg
     1981 ggtatcctgg aactctcccg taccttcgct cacgttgatc cggtgaaaga gccgattccg
     2041 gttatcccaa cctgtcacta catgatgggc ggtattccga ccaaagtgac cggtcaggcg
     2101 ctgacggtga acgagaaagg cgaagatgtg gttgttccgg gactgtttgc cgttggtgaa
     2161 atcgcttgtg tatcggtaca cggcgctaac cgtctgggcg gcaactcgct gctggacctg
     2221 gtggtctttg gtcgtgcagc gggtctgcat ctgcaagagt ctatcgccga acagggcgca
     2281 ttgcgcgatg ccagcgagtc tgatgttgaa gcatctctgg atcgtctgaa ccgctggaac
     2341 aataatcgta acggtgaaga tccggtggcg attcgtaaag cgctgcaaga atgtatgcag
     2401 cataacttct cggtcttccg tgaaggtgat gcgatggcga aagggcttga gcagttgaaa
     2461 gtgatccgcg agcgtctgaa aaatgcccgt ctggatgaca cttccagcga gttcaacacc
     2521 cagcgcgttg agtgcctgga actggataac ctgatggaaa cggcgtatgc aacggctgtt
     2581 tctgccaact tccgtaccga aagccgtggc gcacatagcc gcttcgactt cccggatcgt
     2641 gatgatgaaa actggctgtg ccactccctg tatctgccag agtcggaatc catgacgcgc
     2701 cgaagcgtca acatggaacc gaaactgcgc ccggcattcc cgccgaagat tcgtacttac
     2761 taatgcggag acaggaaaat gagactcgag ttttcaattt atcgctataa cccggatgtt
     2821 gatgatgctc cgcgtatgca ggattacacc ctggaagcgg aagaaggtcg cgacatgatg
     2881 ctgctggatg cgcttatcca gctgaaagag aaagatccca gcctgtcgtt ccgccgctcc
     2941 tgccgtgaag gtgtgtgcgg ttccgacggt ctgaacatga acggcaagaa tggtctggcc
     3001 tgtattaccc cgatttcggc actcaaccag ccgggcaaga agattgtgat tcgcccgatg
     3061 ccaggtttac cggtgatccg cgatttggtg gtagacatgg gacaattcta tgcgcaatat
     3121 gagaaaatta agccttacct gttgaataat ggacaaaatc cgccagctcg cgagcattta
     3181 cagatgccag agcagcgcga aaaactcgac gggctgtatg aatgtattct ctgcgcatgt
     3241 tgttcaacct cttgtccgtc tttctggtgg aatcccgata agtttatcgg cccggcaggc
     3301 ttgttagcgg catatcgttt cctgatcgat agccgtgata ccgagactga cagccgcctc
     3361 gacggtttga gcgatgcatt cagcgtattc cgctgtcaca gcatcatgaa ctgcgtcagt
     3421 gtatgtccga aggggctgaa cccgacgcgc gccatcggcc atattaagtc gatgttgttg
     3481 caacgtaatg cgtaaaccgt aggcctgata agacgcgcaa gcgtcgcatc aggcaaccag
     3541 tgccggatgc ggcgtgaacg ccttatccgg cctacaagtc attacccgta ggcctgataa
     3601 gcgcagcgca tcaggcgtaa caaagaaatg caggaaatct ttaaaaactg cccctgacac
     3661 taagacagtt tttaaaggtt ccttcgcgag ccactacata gacaagagct cgcaagtgaa
     3721 ccccggcacg cacatcactg tgcgtggtag tatccacggc gaagtaagca taaaaaagat
     3781 gcttaaggga tcacgatgca gaacagcgct ttgaaagcct ggttggactc ttcttacctc
     3841 tctggcgcaa accagagctg gatagaacag ctctatgaag acttcttaac cgatcctgac
     3901 tcggttgacg ctaactggcg ttcgacgttc cagcagttac ctggtacggg agtcaaaccg
     3961 gatcaattcc actctcaaac gcgtgaatat ttccgccgcc tggcgaaaga cgcttcacgt
     4021 tactcttcaa cgatctccga ccctgacacc aatgtgaagc aggttaaagt cctgcagctc
     4081 attaacgcat accgcttccg tggtcaccag catgcgaatc tcgatccgct gggactgtgg
     4141 cagcaagata aagtggccga tctggatccg tctttccacg atctgaccga agcagacttc
     4201 caggagacct tcaacgtcgg ttcatttgcc agcggcaaag aaaccatgaa actcggcgag
     4261 ctgctggaag ccctcaagca aacctactgt ggcccgattg gtgccgagta tatgcacatt
     4321 accagcaccg aagaaaaacg ctggatccaa cagcgtatcg agtctggtcg cgcgactttc
     4381 aatagcgaag agaaaaaacg cttcttaagc gaactgaccg ccgctgaagg ccttgaacgt
     4441 tacctcggcg caaaattccc tggcgcaaaa cgcttctcgc tggaaggcgg tgacgcgtta
     4501 atcccgatgc ttaaagagat gatccgacac gctggcaaca gcggcacccg cgaagtggtt
     4561 ctcgggatgg cgcaccgtgg tcgtctgaac gtgctggtga acgtgctggg taaaaaaccg
     4621 caagacttgt tcgacgagtt cgccggtaaa cataaagaac acctcggcac gggtgacgtg
     4681 aaataccaca tgggcttctc gtctgacttc cagaccgatg gcggcctggt gcacctggcg
     4741 ctggcgttta acccgtctca ccttgagatt gtaagcccgg tagttatcgg ttctgttcgt
     4801 gcccgtctgg acagacttga tgagccgagc agcaacaaag tgctgccaat caccatccac
     4861 ggtgacgccg cagtgaccgg gcagggtgtg gttcaggaaa ctctgaacat gtcgaaagcg
     4921 cgtggttatg aagttggcgg tacggtacgt atcgttatca acaaccaggt tggtttcacc
     4981 acctctaatc cgctggatgc tcgttctaca ccgtactgta ctgatatcgg taagatggtt
     5041 caggcaccga ttttccacgt taacgcggat gatccggaag ccgttgcctt tgtgacccgt
     5101 ctggcgctcg atttccgtaa cacctttaaa cgtgatgtct tcatcgacct ggtgtgctac
     5161 cgccgtcacg gccacaacga agccgacgag ccgagcgcaa cccagccgct gatgtatcag
     5221 aaaatcaaaa aacatccgac gccgcgcaaa atctatgctg acaagctgga gcaggaaaaa
     5281 gtggcgacgc tggaagatgc caccgagatg gttaacctgt accgcgatgc gctggatgct
     5341 ggcgattgcg tagtggcaga gtggcgtccg atgaacatgc actctttcac ctggtcgccg
     5401 tatctcaacc acgaatggga cgaagagtac ccgaacaaag ttgagatgaa gcgcctgcag
     5461 gagctggcga aacgcatcag cacggtgccg gaagcagttg aaatgcagtc tcgcgttgcc
     5521 aagatttatg gcgatcgcca ggcgatggct gccggtgaga aactgttcga ctggggcggt
     5581 gcggaaaacc tcgcttacgc cacgctggtt gacgaaggca ttccggttcg cctgtcgggt
     5641 gaagactccg gtcgcggtac cttcttccac cgccacgcgg tgatccacaa ccagtctaac
     5701 ggttccactt acacgccgtt gcaacacatc cataacgggc agggcgcgtt ccgcgtctgg
     5761 gactccgtac tgtctgaaga agcagtgctg gcgtttgaat acggttatgc caccgcagaa
     5821 ccacgcactc tgaccatctg ggaagcgcag ttcggtgact tcgccaacgg tgcgcaggtg
     5881 gttatcgacc agttcatctc ctctggcgag cagaaatggg gccggatgtg tggtctggtg
     5941 atgttgctgc cgcacggtta cgaagggcag gggccggagc actcctccgc gcgtctggaa
     6001 cgttatctgc aactttgtgc tgagcaaaac atgcaggtgt gcgtaccgtc taccccggca
     6061 caggtttacc acatgctgcg tcgtcaggcg ctgcgcggga tgcgtcgtcc gctggtcgtg
     6121 atgtcgccga aatccctgct gcgtcatccg ctggcggttt ccagcctcga agaactggcg
     6181 aacggcacct tcctgccagc catcggtgaa atcgacgagc ttgatccgaa gggcgtgaag
     6241 cgcgtagtga tgtgttctgg taaggtttat tacgacctgc tggaacagcg tcgtaagaac
     6301 aatcaacacg atgtcgccat tgtgcgtatc gagcaactct acccgttccc gcataaagcg
     6361 atgcaggaag tgttgcaaca gtttgctcac gtcaaggatt ttgtctggtg ccaggaagag
     6421 ccgctcaacc agggcgcatg gtactgcagc cagcatcatt tccgtgaagt gattccgttt
     6481 ggggcttctc tgcgttatgc aggccgcccg gcctccgcct ctccggcggt agggtatatg
     6541 tccgttcacc agaaacagca acaagatctg gttaatgacg cgctgaacgt cgaataaata
     6601 aaggatacac aatgagtagc gtagatattc tggtccctga cctgcctgaa tccgtagccg
     6661 atgccaccgt cgcaacctgg cataaaaaac ccggcgacgc agtcgtacgt gatgaagtgc
     6721 tggtagaaat cgaaactgac aaagtggtac tggaagtacc ggcatcagca gacggcattc
     6781 tggatgcggt tctggaagat gaaggtacaa cggtaacgtc tcgtcagatc cttggtcgcc
     6841 tgcgtgaagg caacagcgcc ggtaaagaaa ccagcgccaa atctgaagag aaagcgtcca
     6901 ctccggcgca acgccagcag gcatctctgg aagagcaaaa caacgatgcg ttaagcccgg
     6961 cgatccgtcg cctgctggct gaacacaatc tcgacgccag cgccattaaa ggcaccggtg
     7021 tgggtggtcg tctgactcgt gaagatgtgg aaaaacatct ggcgaaagcc ccggcgaaag
     7081 agtctgctcc ggcagcggct gctccggcgg cgcaaccggc tctggctgca cgtagtgaaa
     7141 aacgtgtccc gatgactcgc ctgcgtaagc gtgtggcaga gcgtctgctg gaagcgaaaa
     7201 actccaccgc catgctgacc acgttcaacg aagtcaacat gaagccgatt atggatctgc
     7261 gtaagcagta cggtgaagcg tttgaaaaac gccacggcat ccgtctgggc tttatgtcct
     7321 tctacgtgaa agcggtggtt gaagccctga aacgttaccc ggaagtgaac gcgtctatcg
     7381 acggcgatga cgtggtttac cacaactatt tcgacgtcag catggcggtt tctacgccgc
     7441 gcggcctggt gacaccggta ctgcgtgatg tcgataccct cggcatggca gacatcgaga
     7501 agaaaatcaa agagctggca gtcaaaggcc gtgacggcaa gctgaccgtt gaagatctga
     7561 ccggtggtaa cttcaccatc accaacggtg gtgtgttcgg ttccctgatg tctacgccga
     7621 tcatcaaccc gccgcagagc gcaattctgg gtatgcacgc tatcaaagat cgtccgatgg
     7681 cggtgaatgg tcaggttgag atcctgccga tgatgtacct ggcgctgtcc tacgatcacc
     7741 gtctgatcga tggtcgcgaa tccgtgggct tcctggtaac gatcaaagag ttgctggaag
     7801 atccgacgcg tctgctgctg gacgtgtagt agtttaagct tcacctgcac tgtagaccga
     7861 cgtggatagg gtgtcggtct acggtttaaa agataacgat tactgaagga tggacagaac
     7921 acatgaactt acatgaatat caggcaaaac aactttttgc ccgctatggc ttaccagcac
     7981 cggtgggtta tgcctgtact actccgcgcg aagcagaaga agccgcttca aaaatcggtg
     8041 ccggcccgtg ggtagtgaaa tgtcaggttc acgctggtgg ccgcggtaaa gcgggcggtg
     8101 tgaaagttgt aaacagcaaa gaagacatcc gtgcttttgc agaaaactgg ctcggcaagc
     8161 gtctggtaac gtatcaaaca gatgccaatg gccaaccggt taaccagatt ctggttgaag
     8221 cagcgaccga tatcgctaaa gagctgtatc tcggtgccgt tgtagaccgt agttcccgtc
     8281 gtgtggtctt tatggcctcc accgaaggcg gcgtggaaat cgaaaaagtg gcggaagaaa
     8341 ctccgcacct gatccacaaa gttgcgcttg atccgctgac tggcccgatg ccgtatcagg
     8401 gacgcgagct ggcgttcaaa ctgggtctgg aaggtaaact ggttcagcag ttcaccaaaa
     8461 tcttcatggg cctggcgacc attttcctgg agcgcgacct ggcgttgatc gaaatcaacc
     8521 cgctggtcat caccaaacag ggcgatctga tttgcctcga cggcaaactg ggcgctgacg
     8581 gcaacgcact gttccgccag cctgatctgc gcgaaatgcg tgaccagtcg caggaagatc
     8641 cgcgtgaagc acaggctgca cagtgggaac tgaactacgt tgcgctggac ggtaacatcg
     8701 gttgtatggt taacggcgca ggtctggcga tgggtacgat ggacatcgtt aaactgcacg
     8761 gcggcgaacc ggctaacttc cttgacgttg gcggcggcgc aaccaaagaa cgtgtaaccg
     8821 aagcgttcaa aatcatcctc tctgacgaca aagtgaaagc cgttctggtt aacatcttcg
     8881 gcggtatcgt tcgttgcgac ctgatcgctg acggtatcat cggcgcggta gcagaagtgg
     8941 gtgttaacgt accggtcgtg gtacgtctgg aaggtaacaa cgccgaactc ggcgcgaaga
     9001 aactggctga cagcggcctg aatattattg cagcaaaagg tctgacggat gcagctcagc
     9061 aggttgttgc cgcagtggag gggaaataat gtccatttta attgataaaa acaccaaggt
     9121 tatctgccag ggctttactg gtagccaggg gactttccac tcagaacagg ccattgcata
     9181 cggcactaaa atggttggcg gcgtaacccc aggtaaaggc ggtactaccc acctcggcct
     9241 gccggtgttc aacaccgtgc gtgaagctgt tgctgctact ggcgctaccg cttctgttat
     9301 ctacgtacca gcaccgttct gcaaagactc cattctggaa gctattgatg caggcatcaa
     9361 actgattatc accatcactg aaggcatccc gacgctggat atgctgactg tgaaagtgaa
     9421 actggacgaa gccggcgttc gtatgatcgg cccgaactgc ccgggcgtta tcactccggg
     9481 tgaatgcaaa atcggtatcc agccgggtca cattcacaaa ccgggtaaag tgggtatcgt
     9541 ttcccgttcc ggtacactga cctatgaagc ggttaaacag accacggatt acggtttcgg
     9601 tcagtcgacc tgtgttggta tcggcggtga cccgatccct ggctctaact ttatcgacat
     9661 tctcgaaatg ttcgaaaaag atccgcagac cgaagcgatc gtaatgatcg gtgagatcgg
     9721 cggtagcgct gaagaagaag cggcagcgta catcaaagag catgttacca agccagttgt
     9781 gggttacatc gctggtgtga ctgcgccgaa aggcaaacgt atgggccacg cgggtgccat
     9841 cattgccggt gggaaaggga ctgcggatga gaaattcgct gctctggaag ccgcaggcgt
     9901 gaaaaccgtt cgcagcctgg cggatatcgg tgaagcactg aaaactgttc tgaaataaat
     9961 atctgtaata agaaatagcc ctcgccgctt ccctctacag gaatggcgaa gggctgtcgg
    10021 tttcgacatg gttggccatc gtatgatggc cttttttgtg cttatcgcga tgattttcgt
    10081 tgcgctatca gggtaaattt atagtcatcg gtattaaaag cgttgcggct atattcaaac
    10141 acccgaccat caactaaata tccacgcgat actttttcaa gaatcggctt tgtctggctg
    10201 atattaagca gacggctcat ctcttcggtt ggcatcagag gaatgatttc ctgttcgcta
    10261 cgatcgataa ccattttctt cacttcttca ataaagtgat attttgaatt ttccatgacc
    10321 tgccaggtga gatccgggaa caacgcaagc ggcatccagg tttcttccag cgccattggc
    10381 ttttgcttgc gatagcgcac gcgcttcaca tgccacacac gatcctgcgc ggtgatttgt
    10441 agctgttgct gaagaaaatc gtcagccgga atcacttcaa atatcaggac ttcactgtgt
    10501 gtatcgacgt gacggtccga cagtttttca tcaaaactgg ttaactgaaa aatatcgtaa
    10561 ttgacccgct cttctttgac gtaagtcccg ctgccctgaa tgctttcgag gatctgttgc
    10621 tcgactaact ggcgcaaagc ctgacgcacc gtaacccggc tgacgccaaa ctctgtttgt
    10681 aatgccgatt cggtaggtaa cgcatcgcca ggtttaagct cgccacgcgc aatttgttca
    10741 cgaatgcgat cggcaatctg ccggtataag ggcttgtgtc ccatttttag tatctcatta
    10801 atacgaattt agccattatg cccgataaat t
//
LOCUS       DBANUU010000146        10788 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000146 DBANUU010000000
VERSION     DBANUU010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.147"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..415
                     /locus_tag="ACIE68_003877"
     CDS             <1..415
                     /locus_tag="ACIE68_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFX5910123.1"
                     /translation="DVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYI
                     SSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINI
                     LTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGFS"
     gene            684..2921
                     /gene="nrfB"
                     /locus_tag="ACIE68_003878"
     CDS             684..2921
                     /gene="nrfB"
                     /locus_tag="ACIE68_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFX5910124.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPNAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQRLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2908..5872
                     /gene="nfrA"
                     /locus_tag="ACIE68_003879"
                     /pseudo
     CDS             2908..5872
                     /gene="nfrA"
                     /locus_tag="ACIE68_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            5873..6763
                     /locus_tag="ACIE68_003880"
     CDS             5873..6763
                     /locus_tag="ACIE68_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFX5910125.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6946..7707
                     /gene="envY"
                     /locus_tag="ACIE68_003881"
     CDS             6946..7707
                     /gene="envY"
                     /locus_tag="ACIE68_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFX5910126.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            8220..9173
                     /gene="ompT"
                     /locus_tag="ACIE68_003882"
     CDS             8220..9173
                     /gene="ompT"
                     /locus_tag="ACIE68_003882"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFX5910127.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            9360..>10788
                     /locus_tag="ACIE68_003883"
     CDS             9360..>10788
                     /locus_tag="ACIE68_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFX5910128.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLIT"
BASE COUNT         2900 a         2674 c         2620 g         2594 t
ORIGIN      
        1 cgatgtccct gatgaactta ttgatttcac gtttgaatgg aaaggtctga agaaattatg
       61 cgtggcagtc tcctttcggt caataatagc agaacaaaag aaagagcccg aaatgacggt
      121 cagatattat atcagttctg ctgatttaac cgcagagaag ttcgccacag caatccgaaa
      181 ccactggcac gtggagaata agctgcactg gcgtctggac gtggtaatga atgaagacga
      241 ctgcaaaata agaagaggaa acgccgcaga attattttca gggatacggc acatcgctat
      301 taatatttta acgaatgata aggtattcaa ggcagggtta agacgtaaga tgcgaaaagc
      361 agccatggac agaaactatc tcgcgtcagt ccttgcgggg agcgggtttt cgtaatcttg
      421 ccctgcctgc tcacctcctc caggcacttc cactacatta tgacctgctc ggtgccaccg
      481 ctccttttca ctatgacgtt aacctcccct ccttgacacc atgcttttgt ctacattcta
      541 cgcaaacggc aacttttata accagtgtaa aaataacgtg ccgcaatagt catacctatt
      601 aatggtaaaa agctgtcaca attcataaaa aaccttaata tacgccaccc taaacataac
      661 cagcgttaat gtaaggtttt tgtgtggact ggcttcttga tgtttttgca acctggctct
      721 acggcttaaa agtaatcgcg ataacgttag cggtcatcat gttcatcagc gggctggacg
      781 atttttttat tgatgtcgtc tactgggtac gccgcattaa acgcaagttg agtgtttatc
      841 gccgctaccc gcgaatgagt taccgcgaac tgtataaacc agatgaaaaa ccgttagcga
      901 ttatggttcc ggcgtggaat gaaacgggcg tcatcggcaa tatggccgag ctggcggcga
      961 ccacgctcga ctacgaaaac tatcatatct ttgttggcac ctaccccaac gaccccgata
     1021 ctcaacgtga tgttgacgaa gtgtgcgctc gctttccgaa cgtgcataag gtagtctgcg
     1081 cgcgtcctgg tcccaccagc aaagccgact gtctgaacaa cgtgctggac gccatcaccc
     1141 agtttgaacg tagcgccaat ttcgcttttg ctggttttat tctgcatgac gccgaagatg
     1201 tgatttcacc gatggaattg cgtctgttca actatctggt cgagcgtaaa gatctgattc
     1261 agatcccggt gtatccgttc gaacgcgaat ggacgcactt caccagcatg acttacattg
     1321 atgagttttc agagctgcat ggcaaagatg ttccggtgcg tgaagccctc gccggacaag
     1381 tgcccagcgc aggcgtcggc acctgtttca gccgccgcgc cgtgaccgct ctgttagctg
     1441 acggtgacgg tattgctttc gacgtgcaga gtctgactga agattacgac attggtttcc
     1501 gcctgaaaga gaaaggtatg acggaaattt ttgtccgttt tccggtggtg gacgaagcca
     1561 aagaacgcga gcagcgcaaa tttttacagc acgcacggac gtcaaacatg atctgcgtgc
     1621 gcgaatattt ccccgatacc ttttcgactg cagttcgaca aaaatctcgc tggatcatcg
     1681 gcattgtttt ccagggcttt aaaacccaca aatggacctc cagcctgacg ctgaactact
     1741 ttctctggcg cgaccgcaaa ggggcaatca gtaactttgt cagcttcctc gcgatgctgg
     1801 tgatgctcca gcttttgctg ttgctggcgt atgaaagttt gtggcccaat gcctggcatt
     1861 tcctttctat ttttagcggc agcgcatggt taatgaccct gctgtggcta aactttggct
     1921 tgatggttaa ccgcatcgtg cagcgggtga ttttcgtcac tggctactac ggtctgacgc
     1981 aggggctgct atctgtcctg cgtcttttct ggggcaacct gattaacttc atggccaact
     2041 ggcgcgcgct aaaacaggta cttcaacacg gcgatccacg tcgcgtcgcg tgggataaaa
     2101 caacgcatga cttccccagc gtgactggcg atacccgctc gttgcgcccg ttaggccaaa
     2161 ttctgctgga aaatcaggtc atcactgaag aacaactcga tacagcactg cgtaatcgcg
     2221 tcgaaggtct acgcctgggc ggttcaatgc tgatgcaggg gctgattagc gccgagcagc
     2281 tggcacaggc gctggcagag caaaacggcg tggcgtggga atccatcgat gcctggcaga
     2341 tcccgtcctc gctgattgcc gaaatgccag cctccgtagc gctgcattat gcagtactgc
     2401 cgctgcgtct ggaaaatgat gagttaattg tcggcagtga agatggtatt gacccggttt
     2461 cgctggcggc cctgacgcgt aaagtcggac gcaaagtgcg ttacgtcatt gttctgcggg
     2521 gacaaattgt cacggggtta cgtcactggt atgcacgccg acgcggtcac gatccgcggg
     2581 caatgttgta caatgcggtt cagcatcagc ggctcacgga acagcagacc ggtgaaatct
     2641 ggcggcaata tgtgccgcat cagttcctgt tcgccgaaat actgaccacg ctcggtcata
     2701 ttaatcgttc agcaattaac gtgttgttat tgcgccatga acgcagttct ctgccgctcg
     2761 gcaagttttt ggtcaccgaa ggcgttatca gccaggaaac gttggatcgc gtcctgacaa
     2821 ttcaacgcga attacaagtt tcgatgcaat cactattact caaagcaggt ttaaacacag
     2881 aacaggttgc gcaactggag tccgaaaatg aaggagaata accttaatcg cgtcatcgga
     2941 tggtctggtt tactgctgac gtctttattg agtaccagcg cactcgcaga caatatcggc
     3001 accagcgcag aagagctggg gctgagcgat tatcgccatt ttgttattta tccccgtctc
     3061 gataaggcgc tgaaggcaca gaaaaataac gacgaagcaa ccgccatccg cgaatttgaa
     3121 tatatacacc agcaggtgcc ggataatatt ccgctgactt tataccttgc ggaagcctat
     3181 cgccattttg gtcatgatga ccgggcgcgg ctgttgcttg aggatcaact gaaacgtcac
     3241 ccaggagatg cccgacttga gcgcagtctg gcggctattc cggttgaagt gaaaagcgtt
     3301 acgactgttg aagaactgct tgcccagcaa aaagcgtgcg acgctgcgcc gaccctgcgt
     3361 tgtcgcagtg aagtcgggca gaatgccctg cggctggcac aattacctgt cgccagagcg
     3421 caactgaacg atgcgacgtt tgctgcatcg ccagaaggaa aaacgctgcg aaccgatctg
     3481 ctgcaacggg caatctacct gaaacaatgg tcccaggcag atacgctata caatgaagca
     3541 cgccagcaga acacattaag cgcggcagaa cgccgtcagt ggtttgacgt gcttcttgcc
     3601 gggcagctgg acgatcggat cctggcactg caatcacagg ggatcttcac cgatcctcag
     3661 tcatatatta cttacgcgac cgcgctggct tatcgtggcg aaaaagcacg cctccagcat
     3721 tatctcaatg aaaataagcc gctgtttacc acggacgcac aagagaaaag ttggctctat
     3781 ctgttatcta aatacagcgc caaccccgtt caggcattgg cgaattatac ggtgcagttt
     3841 gccgataacc gccagtatgt tgttggcgcg acgctaccgg tgctgttaaa agaaggtcag
     3901 tacgacgcag cgcaaaaact gctcgccacc ctccccgcca atgaaatgct tgaggagcgt
     3961 tatgctgtca gcgtggcgac ccgtaacaag gctgaatctc tgcgtctggc acgattgctg
     4021 tatcagcaag aaccggcaaa tcttacccgc ctggatcaac tgacctggca actgatgcag
     4081 aacgagcagt cacgcgaagc tgccgattta ttgctgcaac gctatccttt ccagggcgat
     4141 gcgcgtgtca gccagacttt aatggcgcga ctggcgtctc tgctggaaag tcatccttac
     4201 ctggcaacgc cggcgaaggt ggcgatttta tcgaaaccct taccgctggc ggagcaacgt
     4261 cagtggcaaa gtcagttgcc gggtattgca gataattgcc cggcaatagt tcgcttgctg
     4321 ggcgatatgt cgccttccta cgatgctgcc gcctggaacc gtctggcaaa gtgttatcgg
     4381 gacacgctac ccggtgtggc gctgtatgca tggcttcagg ccgaacaacg acaaccgaac
     4441 gcctggcaac atcgtgcggt agcctatcag gcgtatcagg ttgaggacta cgccaccgca
     4501 ctggcggcct ggcagaaaat cagtcttcac gacatgagca atgaggatct gcttgctgct
     4561 gccaataccg cccaggcggc aggaaatggt gcagctcgcg atcgctggct acaacaggca
     4621 gaacaacgtg gactgggaaa caatgccctc tactggtggc tgcatgcgca acgttacatt
     4681 cctggtcagc cggaactcgc actgagcgat ctcacgcgct caattaatat tgcgccttct
     4741 gccaacgctt acgttgcgcg ggcgacaatt tatcgccaac gtcataatgt cccggcggcg
     4801 gtgagtgatt tgcgcgccgc gctggaactg gaaccgaata atagcaacat ccaggcagcg
     4861 cttggttacg ccttgtggga tagcggtgat atcgcacagt cgcgggaaat gctcgaacag
     4921 gcgcataaag ggcttccgga cgatccggca ctgatccgac aactggccta tgtgaaccag
     4981 cgtctggatg acatgcctgc gacgcagcat tacgcccggc tggtgattga tgacattgat
     5041 aatcaggcgc tgataacccc actgacccca gagcaaaatc agcaacgctt caatttccgc
     5101 cgtttgcatg aggaggtcgg tcgccgctgg acgttcagtt tcgattcttc tatcggcttg
     5161 cgttccgggg caatgagcac cgctaacaat aatgtcggcg gcgcagcgcc agggaaaagc
     5221 tatcgtagct acgggcaact ggaagccgag taccgcatcg gacgcaatat gctgctggaa
     5281 ggcgacctgc tctcagttta tagccgcgtc tttgccgata ccggagaaaa cggggtgatg
     5341 atgccggtga aaaatccgat gtccggcacc ggtctgcgct ggaagccgct gcgcgatcag
     5401 atctttttcc tcgccgtcga acagcagttg ccgctgaacg ggcaaaatgg cgcatccgat
     5461 accatgctgc gcgccagcgc ctcattcttt aatggcggca aatacagggc acccgaacgg
     5521 ttcaggctgg tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat
     5581 tcaggcgtgg acggcagatt atcgcgtcag ctggcatcag aaggtagcta acggacagac
     5641 tattgagcct tacgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc
     5701 gcagcttggc ggagtcgggg tccgctggaa tatctggacc ggcgagacgc actacgacgc
     5761 ctggccgcac aaagtcagtc tcggcgtcga gtatcaacat acctttaagg cgattaatca
     5821 acgtaacgga gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa
     5881 gttcattttc gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg
     5941 tattatctgg caaccacaaa accgagatag tcaggttacc gatacccagt ggcaggggct
     6001 gatgagtcag ttacgtttgc aaggcttcga taccctggtt ttgcaatgga cccgttacgg
     6061 cgatgcattc acccagccag aacagcgcgc gttattgttt aagcgtgccg cagctgcgca
     6121 acaggctggc ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca
     6181 gaaacagtcg tccgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca
     6241 gcaagccaga ttatggagcg ccgcgcctgg cgtaacgccg gatggctggt acatcagcgc
     6301 ggaaattgac gacctgaact ggcgcagcga agctgcccgt cagcctttgc taacatggtt
     6361 aaacaacgcg cagcggctga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt
     6421 tttcgccgga aacatgtcgc ccgatggcta tcgccaactg ctggaacaag ttaaagcaac
     6481 cggcgttaat gtctgggtac aggatggcag cggcgtggat aaacttaccg ctgaacagcg
     6541 tgaacgttat ctacaggcca gcgccgattg ccaaagtccc gcccctgcca gcggcattgt
     6601 ttatgaactg tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga aaccggatgc
     6661 agagatcgcc tcgctgttag cgaaacgttc ctcttgcggt aaagacactc tctatttctc
     6721 tctgcgctat ttgcccgtcg cgcacggcat tctcgagtat taaatctcct ccaggtaagt
     6781 cgggtacgac ctggcttacc tctttcgctc tcgtcagaat tccatcaaga ttaattgcta
     6841 aaaagcgtgc taaagaaaga agatcgttat cggcgaacgg gccgatcact catgcaggaa
     6901 gcatagatcc tttgagcgga gtttctgata tctgaggagt gcgaaatgca attgagcagc
     6961 agtgaacctt gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt caataaccat
     7021 gctacgttta cccttccgaa aaactacctg gccgccttcg cgtgcaacaa taacgtcatt
     7081 gaactctcaa cgttaaatca cgtattaatc ccccacatca accgtaacat catcaacgat
     7141 tatctgttgt ttttaaataa aaacttaacc tgtgtaaagc cctggtcgcg gctggcaacc
     7201 ccggttatcg cttgtcatag ccgtacaccg gaagtgttcc ggctagccgc caaccacagc
     7261 aagcagcaac ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta
     7321 ttgtctaact ttcttgagca atcgcggttt atagccctac tgatgtatat cttacgcagc
     7381 agcgtccgcg acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg
     7441 cgaattgtcg ccagttcgct atgtttaagc cccagcctac tcaaaaagaa attaaaaaac
     7501 gaaaatacca gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg
     7561 ttattgatgg ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg
     7621 tcgtacttta tctctgtttt taaggcgttt tacggcctga caccgttgaa ttatctcgcc
     7681 aaacagcgac aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa
     7741 atgaagaaga aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaagttc
     7801 ctagaacgac attttaagtc aacaaattac cgcgccatct ctgcgctcac acgtcccact
     7861 acctcaaaac atgtaaagcc ttgcaagcca ttgtgaggcc ttatgtgtct cagttttgtc
     7921 cctctttttt gtactaaaaa acatagcaat tgaggataaa cctcatgcta ttttcgctta
     7981 tatgccttta aaggcatggc gattaaatag ataaaaacac cacaaaagca taaaaaacca
     8041 cacagtaaaa ccgcaacata aaacaataac agataattaa accaaaaaca gatagcgcat
     8101 tgtgataatc attcaatact aaacaaaata taaacagtgg agcaatatgt gattgactca
     8161 ttaagttaga tataaaaaat acatattcaa tcattaaaac gattgaatgg agaactttta
     8221 tgcgggcgaa acttctggga atagttctga caacccctat tgcgatcagc tcttttgctt
     8281 ctaccgagac tatatcgttt actcctgaca acataaatgc ggacattagt cttggaactc
     8341 tgagcggaaa aacaaaagag cgcgtttatc tagctgaaga aggaggccga aaagtcagtc
     8401 aactcgactg gaaattcaat aacgctgcaa ttattaaagg tgcaattaat tgggatttga
     8461 tgccccagat atctattggg gctgcgggct ggactactct cggcagccga ggtggcaata
     8521 tggtcgatca ggactggatg gattccagta accccggaac ctggacagat gaaagtaggc
     8581 accctgatac acaactcaat tatgccaacg aatttgatct gaatatcaaa ggctggctcc
     8641 tcaacgaacc caattaccgc ctgggactca tggccggata tcaggaaagc cgttatagct
     8701 ttacagccag aggtggttcc tatatctaca gttctgagga gggattcaga gatgatatcg
     8761 gctccttccc gaatggagaa agagcaatcg gctacaaaca acgttttaaa atgccctaca
     8821 ttggcttgac tggaagttat cgttatgaag attttgagct aggtggcaca tttaaataca
     8881 gcggctgggt ggaagcatct gataacgatg agcactatga cccaggaaaa agaatcactt
     8941 atcgcagtaa agtcaaagac caaaattact attctgttgc agtcaatgca ggttattacg
     9001 taacacctaa cgcaaaggtt tatgttgaag gcgcatggaa tcgggttacg aataaaaaag
     9061 gtaatacttc actttatgat cacaatgata acacttcaga ctacagcaaa aatggtgcag
     9121 gcatagaaaa ctataacttc atcactactg ctggtcttaa gtacaccttt taacgaagtt
     9181 aaccagattt tctccccggt ggtattgtat aaccccgggg aatttgttta cagttacaag
     9241 taggcaatat ccactaattt tgtaagcatt taacctatgt cataacgtgt aatctcttac
     9301 cgcaacacaa gacctcaata tattcaaatc ttatctttat tccgatattc agagacacaa
     9361 tgcgaaaaat aattactcat ttcaaagttg ttttaacgtt acttctacca gtaaccgtat
     9421 ctgcccagca gatacagtgg caatcctgta tggccagtca attcaaccac tggtttggtg
     9481 aggaaaaacc gtctcctgac ttactatgtg gttatttgtc tgttccatta aaatatacag
     9541 acacaggcgg agatgcttct tatgaaaaaa aatcacaagt caaactagcg ttgacaaaat
     9601 tgccggcaaa aagcaagcat aaaggaagta tcctgataat aagtggtggt cccgggttac
     9661 caggcataaa tccttatatt aactttgact ggccagtcac aaatcttcgt gagtcatggg
     9721 atattattgg atttgatcct cgaggcgtcg gacagtccac tccgacaata aactgccggc
     9781 aatcagatac agagactcag gaaaacataa ccgaaaagca acaagtatta aataaaatta
     9841 atgcctgtat ccataatacc ggagccgaag tcattcgcca tatcggctct aacgaggctg
     9901 tatacgatat tgatcgtatt aggcaagcct tgggggataa acaactgaca gccgtggcgt
     9961 attcgtatgg aactcaaatt gcagccttat atgcagaacg ttttccctac aacgtaagat
    10021 ctatcgttct tgatggagtc gtcgatatcg atgacctgga ggacaacttc acatggcaac
    10081 tcaaacaggc acagagttat caggaaacgt ttgatcgctt tgcatcctgg tgtgcgcgta
    10141 caaaaagttg cccgctttct tcagacagag ataaggcaat aactcagttc catgagctat
    10201 tatcaaaatt acatcacaaa cctttattag acagtaaggg agaaaatata tcttcagatg
    10261 aactcatatc attaacaaca gaccttctgc tatggcgttc atcatggcca acccttgcaa
    10321 ctgccatacg ccagttctct caggggattg tcagtaatga aattgaaact gcgctcagtg
    10381 ctccgatagc ctcagaagag tcaagcgatg cttcgggggt aatcctctgt gtagatcagg
    10441 gggatgagca attaacacca gaagagcgaa aatcccgaaa agacgctctt gcgaatgcct
    10501 tcccggctat taactttgac aatggacgtt ccgattcacc tgatttttgt gaattatggc
    10561 caatacatag cgacctgaac aaaactcgcc tgaaaaatac tgttctgccc tctggtttac
    10621 tgtttgtagc acacaaatac gacccaacaa cgccctggat taatgcccgt aagatggcag
    10681 agaaattttc cagcccgtta ctaacaataa atggtgatgg gcatacatta gctctcaccg
    10741 gagttaattt atgtgtagat aaagcagttg tacatcacct gatcactc
//
LOCUS       DBANUU010000147        10707 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000147 DBANUU010000000
VERSION     DBANUU010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.149"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            85..798
                     /gene="yghS"
                     /locus_tag="ACIE68_003884"
     CDS             85..798
                     /gene="yghS"
                     /locus_tag="ACIE68_003884"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFX5910129.1"
                     /translation="MNQRNMSIINSTPVHVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
     gene            830..1588
                     /locus_tag="ACIE68_003885"
     CDS             830..1588
                     /locus_tag="ACIE68_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910130.1"
                     /translation="MDALQTQTVISTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1634..2983
                     /locus_tag="ACIE68_003886"
     CDS             1634..2983
                     /locus_tag="ACIE68_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFX5910131.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPHQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            2983..3807
                     /locus_tag="ACIE68_003887"
     CDS             2983..3807
                     /locus_tag="ACIE68_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910132.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            3819..4379
                     /locus_tag="ACIE68_003888"
     CDS             3819..4379
                     /locus_tag="ACIE68_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFX5910133.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4410..5480
                     /gene="lptF"
                     /locus_tag="ACIE68_003889"
     CDS             4410..5480
                     /gene="lptF"
                     /locus_tag="ACIE68_003889"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFX5910134.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLVQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            5477..6556
                     /gene="lptG"
                     /locus_tag="ACIE68_003890"
     CDS             5477..6556
                     /gene="lptG"
                     /locus_tag="ACIE68_003890"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFX5910135.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(6592..7764)
                     /locus_tag="ACIE68_003891"
     CDS             complement(6592..7764)
                     /locus_tag="ACIE68_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFX5910136.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(7764..8012)
                     /locus_tag="ACIE68_003892"
     CDS             complement(7764..8012)
                     /locus_tag="ACIE68_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFX5910137.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(8044..8958)
                     /locus_tag="ACIE68_003893"
     CDS             complement(8044..8958)
                     /locus_tag="ACIE68_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFX5910138.1"
                     /translation="MNQTVAVTGATGFIGKYIINNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTHCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVPGGYDW
                     QHVRQLAADARCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(8955..10643)
                     /locus_tag="ACIE68_003894"
     CDS             complement(8955..10643)
                     /locus_tag="ACIE68_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFX5910139.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHDNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIELLPPHSIPRTSSGKPARAEAKKRYQKAYAASLHVQESLA"
BASE COUNT         2565 a         2672 c         2810 g         2660 t
ORIGIN      
        1 taaccattgt cacaataagg ttgaacggat attctgagca cggaccacgt aatattcaat
       61 acattattta ctgccgggtt tttcgtgaat caacgtaata tgtcaataat taattccacg
      121 ccagtgcatg ttattgccat tgtaggatgt gatggttcag gtaaatcgac cctcacggca
      181 agcctggtaa atgaactggc agcaagaatg ccaacagaac acatttatct cgggcaatcg
      241 tccgggcgaa ttggcgaatg gatttcacag ctccctgtta ttggcgcacc ttttgggcgt
      301 tatctgcgaa gtaaagcggc acatgtgcac gaaaagccct caacaccgcc tggcaatatt
      361 actgcactgg ttatctatct gctttcctgc tggcgggcgt acaagtttcg caaaatgttg
      421 tgtaaaagcc agcaaggctt tctgctcatc accgaccgct acccgcaggt tgaagtgccg
      481 gggtttcgct ttgatggccc gcaattggca aaaaccacgg gcggtaacgg ttggataaaa
      541 atgttaaggc agcgcgagct gaagctgtac caatggatgg catcttattt gcccgtattg
      601 ttgattcgtc ttggcattga tgaacaaacg gcgtttgcgc gtaaacctga ccaccaactg
      661 gcagcgttac aggaaaaaat cgccgttacg ccgcaactga cattcaatgg tgcaaagatc
      721 cttgaactgg atggtcggca accagccgat gaaattctgc aagcgtcact acgcgcaatt
      781 cacgccgcgc tttcctgatc accaaaatgg ataggggatg atttaacgaa tggatgcact
      841 acaaacacaa actgttatta gtacaaccgc gccgcagccc agctacattc cggggctaat
      901 tgcggtggtc gggtgtgatg gcaccggtaa atccacactg accaccgacc tggtgaaatc
      961 gctgcaacaa cactggcaaa ccgagcggcg ctatctgggg ctgctctccg gcgaagacgg
     1021 cgacaaaatc aaacgattgc cgttggttgg cgtctggctg gaacggcgac tggcggccaa
     1081 atcctcgaaa acccaaagca tgaaaaccaa atctccggcg ctatgggcgg cggtgattat
     1141 gtactgcttc tcgctgcgaa gaatggcgaa tctacgcaag gttcagcgac tggcgcaaag
     1201 tggcgttctg gtggtcagcg atcgcttccc gcaggctgaa atttcgggct tttattatga
     1261 cggaccgggg attggcgtcg aacgtgcgac cgggaaaatc agcatgtttc tggcgcagcg
     1321 cgaacggcgt ttataccaac aaatggcgca atatcgcccg gaattaatta ttcgcctggg
     1381 cattgatatt gagactgcca tctcccgcaa gcctgaccat gactatgccg agctgcagga
     1441 caaaatcggt gtcatgtcga agattggcta taacggcaca aaaattcttg agattgattc
     1501 cagagcgccc tacagcgaag tgctggaaca ggcacagaag gcggtttctc tggttgccat
     1561 cgtttctgac cgccgaagtt taacttaagg ctggcgggat actttttact atcaacgata
     1621 aggtcaggct gaattggcgg gttttaacat caaacattgg tttgcagatg gcgcgtttcg
     1681 caccattatt cgcaatagcg cctggttagg ctccagtaat gtcgtgagcg ccttgctggg
     1741 gctgttggcg ctctcgtgtg ccggtaaagg gatgacgccc gccatgtttg gcgtactggt
     1801 gattgtgcaa tcgtacgcca agtcgatcag cgattttatt aagtttcaga catggcagct
     1861 ggtggttcag tacggaacgc cagccttgac caacaataat ccgcaccaat ttcgcaatgt
     1921 cgtctcattt tccttctcgc tggatatcgt cagcggcgcg gtggcgattg tcggtggcat
     1981 tgccttactg ccattccttt cccattcatt aggtctggat gaccaaagtt tttggctggc
     2041 agcgctctat tgcacgctca ttccttcaat ggcttcctcc acgccgaccg gcattctgcg
     2101 tgcggtagat cgcttcgatt taattgctgt acagcaggcg acgaaacctt ttctgcgcgc
     2161 agcggggagc gtcgtagcct ggtattttga ctttggtttt gcgggttttg ttattgcctg
     2221 gtacgtgtcg aatctggttg gcggcaccat gtactggtgg tttgccgcgc gcgaattacg
     2281 ccgccgaaat atccataatg ccttcaaatt gaatctgttt gagtctgccc gacacatcaa
     2341 aggcgcgtgg agttttgtct ggtcaaccaa cattgcccac tccatctggt cggcgcgtaa
     2401 ctcgtgcagc acagtgctgg tggggatcgt gttaggaccc gctgccgccg ggttatttaa
     2461 aatcgccatg acattcttcg acgccgccgg aacgccagcg ggtttgctgg gtaaaagttt
     2521 ttacccagag gtgatgcgtt tagatccgcg caccaccaga ccgtggttgt taggtgtgaa
     2581 gtccggttta ctggcgggcg gaatcggtat tctggtggcg ctcgcggtgt ttatcgtcgg
     2641 caagccgctc atttcgctgg tgtttggcgt taagtatctc gaagcgtatg acctgattca
     2701 ggtgatgctg ggcgcaattg tgatttcgat gctgggcttc cctcaggagt cattgctatt
     2761 gatggcggga aaacagcgcg cttttctcgt ggcgcaaacc atcgcctcaa tcggctacat
     2821 cgtattgctg ttcatgttct gtcatctgtt tggtgtgctg ggagcagcgt ttgcttactt
     2881 cggtgggcaa tgtctggacg tggcgctctc gttaattcca accttaaagg cattttttca
     2941 gcgccattcc ttgctttata acgcagccgg agagaaatcc tgatgatgag aaaatatttc
     3001 ccgcttgagg cgagcgagcg tctatttgtt gcgattgaag aagatgatgt cgttgatgca
     3061 caggttagtt tgccgccgac tattgcacta agttgtacca ccgaaatcat tcatgataat
     3121 tacgcgttat gcctgcaatt ctggctcaat ggcgttgatc gtcaggaatt actgcgttta
     3181 gttcgcaaac aagccaaagg cgatgagctg actgctgatg agcgcaaaca atttaaatac
     3241 atgcgagcgc gctacaagca cctgcgtttt gcgcaacggc tgtatttaaa gaagcatcag
     3301 gccggatttt tattcggcaa aacgaccgtt tttcttgggc gttttcagga cggctttcgt
     3361 aacggtaaga aaaatattgt gtcgtattac ggcaacctgt tacggattta tttaagctct
     3421 ccggtctggt cgttggtgaa ctattcatta cgccatagcc agttagaaag cgtaagcagt
     3481 tttattgcct atcggcaaaa gcaaatgcat accctgaaag agattattgc caaaccgcga
     3541 ctaaccggca gggaatttca tgatgtgcga aaaatcatca gccaacaggt ttcttattac
     3601 gacaccttaa gatcgcttga tcctgaaaat aaagaggcgt tacagatttc ccgtttcctc
     3661 gccgccatta atggcctgat gggagataaa cacgacgaca tggtggcgga tgatatggaa
     3721 aaccgccagt cgtatgatgc accgttggcg ctggatagcg atattcgtca gcgcttagag
     3781 ctgctgattt cgcgttttcc gctataaccg gagcaatgat gtcatctcgt ttgattattg
     3841 cgctgattat catgctttta gccccgggtg tgcaggcaca caatttcgtc actggcaaaa
     3901 cggttacccc tgtttatatt caggaaggcg gcgagctgct tctgaatagc gacgacgaaa
     3961 ttcactacca aaagtggaac agcacacaat tggcgggaaa agtccgcatt atccagtata
     4021 tcgccgggcg taagtcggcg aagaaaaaga actcactgtt aattaaagcg gttgaagcgg
     4081 caaactttcc gcaggatcgt tttcagccca ccaccattgt gaacaccgat gatgccatct
     4141 ttggtactgg ctattttgtt gttggcaaaa tcgaaaaaaa taaacgccgc tatccgtggg
     4201 cgcaattcgt tattgacggc aatggacagg gacgcgttgc ctggcgcttg ccggagcaga
     4261 gttcaaccat tctggtgctg aataaagccg ggcaaattca gtgggcaaag gacggcagcc
     4321 tgacgccgga agaggtagat catgtcattg cgctggcgca gaaactgatc aatgaataaa
     4381 aaattcaaag gattgagggc gttagtgaga tgaagctggt tgaacactac atcatgcgtg
     4441 gtacgcgccg tctggtgctg attatcgtcg gttttctgat ctttattttc gccagctact
     4501 ccgcacagcg ttatctgacc gaagcggcaa atgggacatt agctcttgat gttgtgctgg
     4561 atatcgtttt ctacaaagtg ctgattgcac tggagatgtt gttacctgtt ggactgtatg
     4621 tgtcagttgg cgtgacgcta gggcagatgt ataccgattc ggaaattacc gctatctctg
     4681 cggcgggggg cagtccggga cgcttgtaca aagccgttct ttatctggcg ataccgctaa
     4741 gtatttttgt cacccttctg tcgatgtatg gtcgtccgtg ggcttatgcg cagatttatc
     4801 aactggagca gcagtcgcag tcggagctgg atgttcgcca gttgcgggca aagaaattta
     4861 acactaacga taacggacga atgatccttt cgcagacggt tgatcaggat aataatcgcc
     4921 tgactgacgc gctgatttat acttctactg ccaatcgaac ccgcattttc cgcgcccgtt
     4981 cggttgatgt ggttgaccca tcacccgaga aaccgaccgt tatgttgcac aacggcactg
     5041 cctatcttct cgatcatcag gggcgtgacg acaacgaaca gatctaccgt aatctgcaat
     5101 tacatctgaa tccgctggtt caaagcccta acgtcaaacg caaagcgaaa tcggtcacgg
     5161 agctggcgcg ctccgtcttt cctgccgatc atgccgaact gcaatggcga caaagccgtg
     5221 gtctgacagc attgttgatg gcgctgctgg ccatttcatt aagtcgggta aaaccgcggc
     5281 aagggcgatt ttcaacgtta ttgccactga cgttgctgtt tattgccatt ttttatggcg
     5341 gcgacgtctg ccgtacgctg gtggctaacg gcgcgattcc cctcattcct ggtttgtggt
     5401 tagtgcccgg actcatgcta atgggcctgc tgatgctggt cgcacgcgac ttctctttgc
     5461 tgcagaaatt ttcccgatga atgttttcag tcgctattta atccgtcatc tctttctcgg
     5521 ttttgccgcc gccgcagggc tattgctgcc gctttttacc accttcaact tgattaacga
     5581 actggatgat gtcagcccgg gcggttatcg ctggactcag gcggtgctgg tggtgctaat
     5641 gaccttaccg cgcacactgg tcgaactttc gccgtttatc gcgttattgg gtggtattgt
     5701 tggactgggg cagttatcga aaaacagtga gcttaccgcc attcgcagca cggggttttc
     5761 tatcttccgt attgcactgg tggcgctggt tgccgggata ttgtggactg tttcgttagg
     5821 cgcgatagat gagtgggtgg cgtcgccatt gcagcaacag gcgttgcaaa tcaaatcgac
     5881 cgccaccgcg ttgggggagg acgatgacat taccggcaat atgctgtggg ccaggcgtgg
     5941 taatgaattt gtgacggtga aatcgctgaa cgagcagggc cagccggtgg gcgtggagat
     6001 atttcattat cgcgacgatc tctcgcttga atcctacatt tatgcacgca gtgccaccat
     6061 taaagacgac aaaacgtggg tcctgcatgg tgtgaatcat aaaaaatggc tgaatggcaa
     6121 agaaacgctg gaaacattag ataatcttgc ctggcaatcg gccttcacca gtatgaatct
     6181 tgaagagtta tcaatgccgg ggaatacttt ttctgtccgt cagcttaatc attacatcca
     6241 ttatttgcag gaaaccgggc aacccagcag cgaataccgc cttgcactgt gggaaaaact
     6301 ggggcaaccc atcctgaccc tggcgatgat tttgctggct gtgccgttta cctttactgc
     6361 cccgcgttcg cctgggatgg gtagccgtct cgctgtaggc gtcatcgtgg gcttactcac
     6421 ctggatcagc tatcaaatca tggttaatct gggattgcta tttgcgttaa gcgcacctgt
     6481 taccgcgctc ggtttaccgg tagcgtttgt gttggtggcg ttgagcctgg tgtattggta
     6541 tgacagacaa cattaaaccc gccgtggcgc aaacaccacg gcgggctcac attactgctt
     6601 cagagtagca aatgcctgaa tgatggcatc gatttgctca cgactgtggg cagcatttac
     6661 gctacagcgc agcagcgtga tgcctgcagg cgcggcaggc ggcaggataa gattcacata
     6721 cacgcccagc gagataagct cgcgccagat gcgtaaacct tcctctttcg aaccgattat
     6781 tactggcact accgggctaa tatgtgaact taactcataa cccagttttt gcagaccgtc
     6841 atacagatgg ttggcgttat ccatcagctt ttgccgcagc tctggttgtg tggcaatagt
     6901 tcttaacgac gagcgcacgg tcgcaatgca ggacggagaa ggagaggcgg taaaaatata
     6961 cgggcggctg ccgtagcgca gcacttccat cgcttccgaa ccgacagcaa aaccgccaat
     7021 cgaggccaga cttttactga aagtaccaac gataatatcc acatcatctt caacgccgac
     7081 agcttcagcc agtccgcgcc ccgttgcgcc taacacgcca aacgaatgcg cttcatcgac
     7141 aatcaaataa ccaccgagac gacgtttgat atcaacaatt tccgccagcg gcgcaacgtc
     7201 accgagcatg ctgtagatgc cctcaacgat gataatcgcc tctttcgccc gttcaccgag
     7261 gcgaaccatg cgccgttcca gatctttagc atcgttatgg cggaaacgaa taatttccgc
     7321 gccaccgaga gaacaggcgt cataaatact ggcgtggctg tcggcatcca gtaacaccac
     7381 ggcgttatgg tcggcaagtg cgctgatgac gcctaaatta gcggtataac cggttgaaaa
     7441 aacgatagcg gtagggcgat tgaaaaactc ggcaatctct ttctctaacg ccaggtgcgg
     7501 cgcataactg ccgtttgcca tacgagaacc ggtggtgccg gtgccttggg ccgctaacgc
     7561 tgcctgacct tcgctgatgg catcatgatt aaaggttaaa ccgagataat tattggtccc
     7621 ggccagaatc acttttttat tgccgatccg gccttcggtg gcggaataca cttcatcaat
     7681 acaggtgcca aacggtgtta acccgttgtc actaaattgc agtcgctcgc cagcaaggcg
     7741 cgcatattta tcgtatagcc ccattattta ttctccagcc atggaagtag agtttccatt
     7801 aattgcgcag gggttttgac atccagcaga atattaatag gaatggaaat atcgaatctg
     7861 tcttccagca tcattaagag atccatcact ttgatcgact ccaggccaag atcgttgacg
     7921 agatcgctgt cgggcttaat ttccacaccg ttttcgacca aatcctgtaa acaggaaagg
     7981 atgtaatcca ttactatttc acgatttacc atagaaacgt taacacacct ttaaacaaga
     8041 caatcaaaat agcccgttgc gtaatgcgtg ttccaggcta atcccgggaa accaattaat
     8101 atcttcagaa atacggttat tacttgccga ccagtcggcg tgggttaatt cacgaatttt
     8161 cgagcgggtc agcataggtt ctttacctgc caggcgactc aacgcggtac tgatatccgc
     8221 aaggcaggtg agtaccggca gaggaatgcc aaccattcgc acggaaccac aacgggcgtc
     8281 ggcggcaagc tgtcgtacgt gttgccagtc atagccgccg gggacgccat cgcataattc
     8341 ataggtttgc gtctgtacag tttcggcgct taaccactga cccactgctt gcgcgaaatc
     8401 ggtgacgtgc aggaaagaga gctgtgtatc tggcgcacca agtcgtggca gcaggccgcg
     8461 cagcatccag tcaaacagcg gttttaactc tttatcgccg ggaccataca cggctgtcgg
     8521 gcgaaaaacg cccagcgtaa tttcgtcagc cattgcagtc agacgttgtt cggcgacgtg
     8581 tttggaattt gcgtaccagg agagctcggg atggcgcgcc gccagcgaag agataaacag
     8641 aaaacgttgg caaaagccgc tctcttttgc tgcttgcatc aggcgcaggc tgccgtcaac
     8701 gttacagtgg gtaaaaatct cttctttgtg cccgcgcact tgcccggcgc aatggaccac
     8761 cgcgctggct ccggcaacca gcttgctaag tgaatgagta tcttccagcg aaccgcgcac
     8821 ccaggtgaga ttatcattga cgtgagcgcg ggcagtacgc gtcaatgcgc gaacgtgaaa
     8881 gccgcgggcg agcaggttat tgataatata tttaccgata aacccggtag cgcccgtcac
     8941 cgcgacagtt tgattcatgc cagggattcc tgcacatgaa gactggcagc ataagccttc
     9001 tgataacgtt ttttcgcttc cgcacgggca ggcttgccgg aggacgttcg gggaatactg
     9061 tggggcggca acagctcgat agccgcggtc acgccaaatt cgctttggat ccgtgccgcc
     9121 agcgcgtgga taagctgccc gcgacgttct tcgtcgctga tccgacactg gatctgcaaa
     9181 atgatttttt cctgggcggt aacaaaagca atcgcatcgc cagaatgaat ttccggttct
     9241 tgttccgcaa tatattcaat atcctgcggc cagatattac ggccacgaat aataatcaga
     9301 tctttaatgc gtccggtgac atacagataa ccgtccagca gataaccgag gtcgccggtg
     9361 tctaaccagc ccgtcgcggc aatctcgtct tgcgaagcct ggtcgccaaa gtaaccgctc
     9421 atcagactgg gaccggagat gcaaatatgg cctaccacac gttccgcgac cggcatacct
     9481 gcttcattgc ggatttcaat accatgttcc ggcaacgctt tgccgcagtt gacgaaagtc
     9541 gatacggcgc gtgtctctgc acccggcgcg acggctttac cctgatattc gaggatgtcg
     9601 cgatccactt cgttaaccac aaccccggag gcttcatcag agaagctgac agccagcgca
     9661 ttttctgcca gtccgtagca cggcatgaaa gttttattgt caaagttaac ctgacggaaa
     9721 cattcagcga attgatggag ttgttctgcg gagatgggtt ctgcaccaat accagcgacg
     9781 cgccagcagg aaagatccag ttcagcgaga tctttttcat tcacgcggcg ctggcacaat
     9841 tcatagccaa acggcggcgc aacggaaacg gtgccgcgat ttttactgat caatttaagc
     9901 cattgcagag gacgcatggc aaaatcctga gtgcgcaaat aatctactga aagctgcgtg
     9961 gcgacggggg tcaggagaaa gccgaccagt cccatatcat ggtagaaagg cagccaggag
    10021 acgcagcggt cgccagggcg taatttaatg ccgtcgtggc ttatagcacg tagattagcc
    10081 atcacttcgc gatgggtgat aatgacgcca cggggaaaac gggtgctgcc ggaggtgtac
    10141 tggaggtagg cgatatcgtt cggaactgga cgctggagcg caacatcggc ttccggtaat
    10201 gccttaaacc aggcgtggct taaaacatgt aattcggggt tgtcatgcgt cgcggcattg
    10261 accagtggca accactcatc accagtgata atggctgcgg gctggcaact tgccagtaaa
    10321 ccctgcaatt tggcgctcca ggaatcccgc tgaccaacgc ccattggaat cgccaacggg
    10381 acggcgacta agccggcata ctggcaggca aaaaaagcct ctacgaaccc gctacttgtt
    10441 tcggcaatca gtgccacgcg atcgcctttt ttcaggttca gcgataacaa ccgcttcgca
    10501 ccagcttcgg cacgcgtttt taatgtctga tattccagtt gatcttcaag ttggcaacgt
    10561 ctgtcataaa aattcattcc ggcgctactc agagcggcgt agtccaaagc atcaaccagc
    10621 gttggaaaat cggcatagcg cataggtagg gaatgcgtaa agattttatt agacatatac
    10681 accacgaaaa aattggatat caaaaat
//
LOCUS       DBANUU010000148        10693 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000148 DBANUU010000000
VERSION     DBANUU010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.150"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..1070
                     /gene="manD"
                     /locus_tag="ACIE68_003895"
     CDS             <1..1070
                     /gene="manD"
                     /locus_tag="ACIE68_003895"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFX5910140.1"
                     /translation="HLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMAL
                     WDIKAKAANMPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCG
                     VPGMQTTYGLAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHL
                     LHDMHHRLTPIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFN
                     SIWDCKQLIEEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHA
                     AALHFDLWVPNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAK
                     YPYDPAYLPVARLEDGTLWNW"
     gene            1137..2420
                     /locus_tag="ACIE68_003896"
     CDS             1137..2420
                     /locus_tag="ACIE68_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5910141.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            2672..3589
                     /gene="oxyR"
                     /locus_tag="ACIE68_003897"
     CDS             2672..3589
                     /gene="oxyR"
                     /locus_tag="ACIE68_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFX5910142.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3572..4972)
                     /gene="sthA"
                     /locus_tag="ACIE68_003898"
     CDS             complement(3572..4972)
                     /gene="sthA"
                     /locus_tag="ACIE68_003898"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFX5910143.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            5303..5953
                     /gene="fabR"
                     /locus_tag="ACIE68_003899"
     CDS             5303..5953
                     /gene="fabR"
                     /locus_tag="ACIE68_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFX5910144.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5953..6312
                     /gene="yijD"
                     /locus_tag="ACIE68_003900"
     CDS             5953..6312
                     /gene="yijD"
                     /locus_tag="ACIE68_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFX5910145.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLATACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(6352..7452)
                     /gene="trmA"
                     /locus_tag="ACIE68_003901"
     CDS             complement(6352..7452)
                     /gene="trmA"
                     /locus_tag="ACIE68_003901"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFX5910146.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      7566..7756
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            7821..9665
                     /gene="btuB"
                     /locus_tag="ACIE68_003902"
     CDS             7821..9665
                     /gene="btuB"
                     /locus_tag="ACIE68_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFX5910147.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHFGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            9610..10467
                     /gene="murI"
                     /locus_tag="ACIE68_003903"
     CDS             9610..10467
                     /gene="murI"
                     /locus_tag="ACIE68_003903"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFX5910148.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2459 a         2563 c         2873 g         2798 t
ORIGIN      
        1 atcatctttg tccacaacta attggccgcg atgccagccg tatcgaagat atctggcaat
       61 tcttttataa aggtgcttac tggcgacgtg gccccgtcac gatgtcagcc atttctgcca
      121 ttgatatggc gttgtgggat atcaaagcta aagcggcaaa tatgccattg tatcaactgc
      181 ttggtggtgc ttctcgtgaa ggcgtgatgg tttattgcca caccaccggg cggacgattg
      241 atgaagtcct tgaagactat gcaaagcacc agcagatggg ctttaaggcg attcgtgtgc
      301 agtgcggtgt accgggtatg caaactactt atggcctggc gaagggcaaa ggcctggcgt
      361 atgaaccggc aaccaaaggc ctgtggccag aagagcagtt gtggtcttca gaaaagtatc
      421 tcgattttac gccgaagtta tttgaagcgg tgcgtaataa gttcggtttt aacgaacatc
      481 tgttgcatga catgcaccat cgcctgaccc ccatcgaagc ggcgcgcttt gggaagagca
      541 ttgaggatta ccgcctgttc tggatggaag atccgacgcc tgcagagaac caggaatgtt
      601 ttcgtctgat tcgccagcat accgtgacgc cgattgcggt cggtgaagtg tttaacagta
      661 tctgggactg caagcagtta atcgaagagc agctgattga ctatattcgc acgacgatca
      721 cccatgcagg cggcattacc gggatgcggc ggattgccga ttttgcatcg ctttatcagg
      781 tacgtaccgg ttcgcatggc ccttcagatt tatcgcctgt ttgtcatgcg gcggcgctgc
      841 attttgacct atgggtacca aattttggcg ttcaggagta catgggctat tccgaacaaa
      901 tgcttgaagt tttcccgcac agctggcgct tcgaggaagg ttatatgcac ccaggcgatg
      961 aaccggggct gggtatttct ttcgatgaga aactggcggc gaaatatccg tatgacccgg
     1021 cgtatctccc cgtcgcacga ctggaagatg gcacattgtg gaactggtaa taacggttcg
     1081 cttaattatt ttctgaatag tcatcaaatc gattaattcc gctcaaggag ctaaaaatgg
     1141 acgttgatgt gtcaaccagt gttgcgggta ataaaccgca acggattcgt cgtattcaga
     1201 cagtgaccct ggttttatta tttatggcgg ggatcgttaa ttttctcgac cgttcgtcat
     1261 tgagcgtggc aggtgaagcg attcgtggcg agttaggatt atcggcgaca gagtttggtg
     1321 ttttgctttc tgcattttct ctgtcttatg gtttttcaca actaccttcc ggtattttgc
     1381 tagaccgctt tggcccacga attgttttag gcgcaggctt aatattctgg tcattaatgc
     1441 aggcattaac aggaatggtt aattctttta gccactttat tttaatgcgt atcggtctgg
     1501 gtattggcga agcgccattt atgcctgcgg gagtaaagtc gatcaccgac tggtatgcac
     1561 aaaaagaacg cggcacggcg ctggggattt ttaactcatc taccgttatc ggtcaggcta
     1621 tcgcgcctcc tgctcttgta ttgatgcagc tggcatgggg ctggcggacg atgttcgtta
     1681 tcatcggcgt ggcagggatt ctggttggga tctgttggta cgcgtggtat cgcaaccggg
     1741 cgcagtttgt cctgactgac gaagaacgga cgtatctctc cgcgccggtt aaaccgcgtc
     1801 cacagctgca atttagcgag tggctggcgc tgtttaagca tcggacaacc tgggggatga
     1861 ttttaggttt ctctggcgtc aattataccg gttggctcta catcgcgtgg ttacccggtt
     1921 atttgcaagc cgagcaaggt ttcagtctgg cgaaaaccgg ttgggtggcg gcgattcctt
     1981 tcctcgcggc ggcagtcggg atgtgggtta acggtattgt tgtcgatcga ctggcgaaaa
     2041 aaggctacga cctggcgaag acgcgtaaaa cggctattgt ctgcggtttg atgatgtcgg
     2101 cattaggcac gttgctggtc gtgcaatctt cctcgccagc ccaggcggta gcgtttatct
     2161 caatggcgct attctgcgtg catttcgctg gaacgtctgc atgggggctg gtgcaggtga
     2221 tggtgtcaga aacaaaagtg gcttccatcg ccgggattca aaactttggc agttttgttt
     2281 ttgcttcctt tgctccgatc gtaaccggtt gggtagtgga taccacacac tcgtttaatc
     2341 tggcgttggt tatcgcggcc tgtgtgacgt tcaccggggc gctgtgttac ttctttattg
     2401 tcaaagatcg cattgagtaa tcgttactat caggctctct tgctgtgggc ctgtagaata
     2461 aaaaaaagcg gatcctggag atccgcaaaa gttcacgttg gctttagtta ttcgagttga
     2521 gaaactctcg aaacgggcag tgacttcaag ggttaaaaga ggtgccgctc cgtttctgtg
     2581 agcaattatc agtcagaatg cttgataggg ataatcgttc attgctattc tacctatcgc
     2641 catgaactat cgtggcgatg gaggatggat aatgaatatt cgtgatcttg agtacctggt
     2701 ggcattggcc gagcaccgcc attttcggcg tgcggcagat tcctgccacg ttagccagcc
     2761 gacgcttagc gggcaaattc gtaagctgga agatgagttg ggcgtgatgt tgctggagcg
     2821 gaccagccgt aaagtgttgt tcacccaggc gggaatgctg ctggtagatc aggcgcgtac
     2881 cgtactgcgt gaggtgaaag tccttaaaga gatggcaagc cagcagggcg agacgatgtc
     2941 cgggccgctg cacattggtt tgattcccac agttggaccg tacctgctac cgcatattat
     3001 ccctatgctg caccagacct ttccaaagct ggaaatgtat ctgcatgaag cacagaccca
     3061 ccagttactg gcgcaactgg acagcggcaa actcgattgc gtgatcctcg cgctggtgaa
     3121 agagagcgaa gcattcattg aagtgccgtt gtttgatgag ccaatgttgc tggctatcta
     3181 tgaagatcac ccgtgggcga accgcgaatg cgtaccgatg gccgatctgg caggggaaaa
     3241 actgctgatg ctggaagatg gtcactgttt gcgcgatcag gcaatgggtt tctgttttga
     3301 agccggggcg gatgaagata cacacttccg cgcgaccagc ctggagacgc tgcgcaacat
     3361 ggtggcggca ggtagcggga tcactttact gccagcgctg gctgtgccgc cggagcgcaa
     3421 acgcgatggg gttgtttatc tgccgtgcat taagccggaa ccacgccgca ctattggcct
     3481 ggtttatcgt cctggctcac cgctgcgcag ccgctatgag cagctggcag aggccatccg
     3541 agcaagaatg gatggccatt tcgataaagt gttaaaacag gcggtttaaa ccgtttaacg
     3601 cagctacccg ataggcttcc gccatggtcg ggtagttaaa ggtggtgttg acgaagtact
     3661 caatagtgtt gccgccacct ttctgttcca taatcgcctg accgatatga ataatttcgg
     3721 cagcgcgctc gccaaagcag tgaataccca gaatctcttt tgtttcccga tggaacaaaa
     3781 ttttcagcgt gcccacgttc atgccgacga tttgtgcgcg tgccagatgt ttaaactggg
     3841 cgcggcccac ttcatatggc actttcatcg cggtcagctg ctgttcggtt ttgcccacag
     3901 agctgatttc cgggatggtg taaatgccgg tagggatatc ttcaatcaga tgcgcggtgg
     3961 cttcgccttt taccagcgcc tgcgcggcaa tgcgcccctg gtcataggcc gctgatgcca
     4021 ggctcggata accaatcacg tcgcccaccg cgtaaacgtg cggctgtgcg gtctgataca
     4081 tgctgttgac cttcagctgt ccgcgactgt cagtttccag cccaatgttc tgtaacgcca
     4141 gcgaatcggt attaccggta cgaccgttgg catagagcag gcagtcagct ttcagttttt
     4201 tacccgactt cagatgcatg atcacaccgt cgtcacagcc ttcgatcttc tcgtactctt
     4261 cgttgtgacg aatcactacg ccactgttcc agaagtgata ggagagagaa tctgacatct
     4321 cttgatcgag aaatgccagc aggcgatcgc gggtgttgat cagatccact tttacatcca
     4381 taccgcggaa gatcgacgca tattcacagc cgatcactcc agcaccatag ataagtacat
     4441 ggcgcggttc gtggtgcatg ctaagaattg agtcgctgtc gtaaatgcgt ggatgggtga
     4501 aatcaacatc tgttggatga tatggacgag agccgcaggc aataacaaat ttttcagcgg
     4561 ttagtgtttc aacgctgccg tccgggcaat ccagcgccaa cgtatgctcg tcaacaaagc
     4621 gagcgtttcc ctgcaatatt tcacagtgat tacgttcgta aaatccctga cgcatgcgcg
     4681 tttgttgatt aatcacgtta tcggcatggt taaggatatc ggcaaaagaa gagcggagca
     4741 gtcgggaatg gtcgctgtaa agtgggtttt gattgaattc tataatgcgg ctgacggcgt
     4801 gacggagagc tttcgacggg atggtgcccc agtgggtgca accgccgcca acattttgat
     4861 aacgctcgat aactgcgacg cgcgcacctt gcttaaccag gcccattgca gcgccttcgc
     4921 cgccggggcc ggaacctatt actatggcat cgtaatcgta ggaatgtggc atggtagggc
     4981 ttacctgttc ttatacataa aagcaacaga atggtaacat tttatcgcgg gtaagccaat
     5041 tgatccccgt catttatctg gctatatcct gagcggcctt tgctttgtct gtttcttact
     5101 tttgccctga cgttttattg gatttttatc gacgatactc tccgtttaag cggcaggttt
     5161 ccgctgtacg taaaagaacc ggccaaagaa ttgcagtaaa tatgttttat tgcgttaccg
     5221 ttcattcaca atactggagc aatccagtat gttcattctc tggtatagtg ccagcagtac
     5281 ttttggcaag gattcagaca tcgtgatggg cgtaagagcg caacaaaaag aaaaaacccg
     5341 ccgttcgctg gtggaagccg catttagcca attaagtgct gaacgcagct tcgccagcct
     5401 gagtttgcgt gaagtggcgc gtgaagcggg cattgctccc acctcttttt atcggcattt
     5461 ccgcgacgta gacgaactgg gtctgaccat ggttgatgag agcggtttaa tgctacgcca
     5521 actcatgcgc caggcgcgtc agcgtatcgc caaaggcggg agtgtgatcc gcacctcggt
     5581 ctccacattt atggagttca tcggtaataa tcctaacgcc ttccggttat tattgcggga
     5641 acgctccggc acctccgctg cgtttcgtgc cgccgttgcg cgtgaaattc agcacttcat
     5701 tgcggaactt gcggactatc tggaactcga aaaccatatg ccgcgtgcgt ttactgaagc
     5761 gcaagccgaa gcaatggtga caattgtctt cagtgcgggt gccgaggcgt tggacgtcgg
     5821 cgtcgaacaa cgtcggcaat tagaagagcg actggtactg caactgcgaa tgatttcgaa
     5881 aggggcttat tactggtatc gccgtgaaca agagaaaacc gcaattattc cgggaaatgt
     5941 gaaggacgag taatgaaaca agcaaatcaa gatagaggta cgctgctgct ggcgttggtt
     6001 gctggcttat cgattaatgg tactttcgca gcgctgttta gctccattgt gccattttct
     6061 gtattcccga ttatttccct ggtgctgacg gtttactgcc tgcatcaacg ttatcttaat
     6121 cgcaccatgc cggtaggctt gccgggtctg gcaactgcct gttttattct cggcgtactg
     6181 ctgtacagca cggtagttcg tgcggaatat ccggatatcg gctctaactt cttcccggca
     6241 gtactctccg tcattatggt gttctggatt ggcgcgaaga tgcgtaaccg taagcaggaa
     6301 gttgctgagt aatcggtatt atgccggatg ctggcgcatc cggcatgggt tttacttcgc
     6361 ggtcagtaat acgccgcact ccatatggtg cgtgtagggg aactgatcaa acagagccag
     6421 acgttcgacc ttgtgcgtct ggcttaatgt ttccagattc ttgcataacg tttccggatt
     6481 gcaggagata tacagaatac gtggatacgc ctgcaccatt ttctcggttt cactgtccag
     6541 accgctgcgc ggtgggtcga caaaaatggt ttcgcactga taactcttca agtctatccc
     6601 ttgcaggcga ttaaactcgc gtacaccatt catcgcctga gtaaattctt ctgccgccat
     6661 acgaataatt tgtacgttat caatatggtt agctgcgatg ttgtattgcg cagcagcaac
     6721 cgacggctta gcgatttcgg tggctaatac ccgatcaaaa ttgcgcgcca gtgctaatga
     6781 aaagttaccg ttgccgcagt acagctccag taaatcgcct tttgagcctt tggttacgtc
     6841 cagcgcccat tccagcatct gaatattcat cgccgcgttc ggctgggtaa aactgttttc
     6901 tacctgacgg tagatcatct ctttccctgc gaccggcaga cgttcatcga tgtaatcttg
     6961 atccagctcg attttggttt tcgttgcccg accaatcaga tgcacattca gattctgcgc
     7021 gcgcagtgca tcgcgcaggg cttccgcttc ctgacgccac tcatcatcca gctttttatg
     7081 gtacaacagg gaaaccaccg cctgattact cagcgtggtg aggtaatcaa tctggaacaa
     7141 cttgtggcgc agaacgggat tattacgcac acccgcaatc atcgccgtca tcaactggtt
     7201 gatgagttca ctggcagcgg ggaagctatc cacgcggatg cggcttttgg tttgttgatc
     7261 gaaaatgatg tgatacaggt catcgccatc gtgccagatg cggaactccg cacgcatccg
     7321 gtaatgactg accggcgagc gaaacacttc cggaaccagg tcagaaaacg gtgccatcat
     7381 actttgcaaa cgtaccactt tttcggctaa ctgcgcttca tactgttctg ttggaaggtg
     7441 ttcgggggtc atgatgtatc ctgaaaaatt aaagtacgcg gcgattgtag ggattgctca
     7501 tcagatgtcc agatcttgat gaattcctat ttgtgagcta cgtctggaca gtaacttgtt
     7561 acaacctgta gcatccactt gccggtcctg tgagttaata gggaatccag tgcgaatctg
     7621 gagctgacgc gcagcggtaa ggaaaggtgc gatgattgcg ttatgcggac actgccatcc
     7681 ggtgggaagt catcatctct tagtatctta gatacccctc caagcccgaa gacctgccgg
     7741 ccaacgtcgc atctggttct catcatcgcg taatattgat gaaacctgcg gcatccttct
     7801 tctattgtgg atgctttaca atgattaaaa aagcttcgct gctgacggcg tgttctgtca
     7861 cagccttttc cgcttgggca caggatacca gcccggatac tctcgtcgtt actgctaacc
     7921 gttttgaaca gccgcgcagc actgtgcttg caccaaccac cgttgtgacc cgtcaggata
     7981 tcgaccgctg gcagtcgacc tcggtcaatg atgtgctgcg ccgtcttccg ggcgtcgata
     8041 tcacccaaaa cggcggttca ggtcagctct catctatttt tattcgcggt acaaatgcca
     8101 gtcatgtgtt ggtgttaatt gatggcgtac gcctgaatct ggcgggggtg agtggttctg
     8161 ccgaccttag ccagttccct attgcgcttg tccagcgtgt tgaatatatc cgtgggccgc
     8221 gctccgctgt ttatggttcc gatgcaatag gcggggtggt gaatatcatc acgacgcgcg
     8281 atgaacccgg aacggaaatt tcagcagggt ggggaagcaa tagttatcag aactatgatg
     8341 tctctacgca gcaacaactg ggggataaga cacgggtaac gctgttgggc gattatgccc
     8401 atactcatgg ttatgatgtt gttgcctatg gtaataccgg aacgcaagcg cagacagata
     8461 acgatggttt tttaagtaaa acgctttatg gcgcgctgga gcataacttt actgatgcct
     8521 ggagcggctt tgtgcgcggc tatggctatg ataaccgtac caattatgac gcgtattatt
     8581 ctcccggttc accgttgctc gatacccgta aactctatag ccaaagttgg gacgccgggc
     8641 tgcgctataa cggcgaactg attaaatcac aactcattac cagctatagc catagcaaag
     8701 attacaacta cgatccccat tttggtcgtt atgattcgtc ggcgacgctc gatgagatga
     8761 agcaatacac cgtccagtgg gcaaacaatg tcatcgttgg tcacggtagt attggtgcgg
     8821 gtgtcgactg gcagaaacag actacgacgc cgggtacagg ttatgttgag aatggatatg
     8881 atcaacgtaa taccggcatc tatctgaccg ggctgcaaca agtcggcgat tttacctttg
     8941 aaggcgcagc acgcagtgac gataactcac agtttggtcg tcatggaacc tggcaaacca
     9001 gcgccggttg ggaattcatc gaaggttatc gcttcattgc ttcctacggg acatcttata
     9061 aggcaccaaa tctggggcaa ctgtatggct tctacggaaa tccgaatctg gacccggaga
     9121 aaagcaaaca gtgggaaggc gcgtttgaag gcttaaccgc tggggtgaac tggcgtattt
     9181 ccggatatcg taacgatgtc agtgacttga tcgattatga tgatcacacc ctgaaatatt
     9241 acaacgaagg gaaagcgcgg attaagggcg tcgaggcgac cgccaatttt gataccggac
     9301 cactgacgca tactgtgagt tatgattatg tcgatgcgcg caatgcgatt accgacacgc
     9361 cgttgttacg ccgtgctaaa cagcaggtga aataccagct cgactggcag ttgtatgact
     9421 tcgactgggg tattacttat cagtatttag gcactcgcta tgataaggat tactcatctt
     9481 atccttatca aaccgttaaa atgggcggtg tgagcttgtg ggatcttgcg gttgcgtatc
     9541 cggtcacctc tcacctgaca gttcgtggta aaatagccaa cctgttcgac aaagattatg
     9601 agacagtcta tggctaccaa actgcaggac gggaatacac cttgtctggc agctacacct
     9661 tctgaaccac gtcccaccgt gctggtgttt gactccggcg tcggtgggtt gtcggtctat
     9721 gacgagatcc ggcatctctt accggatctc cattacattt atgctttcga taacgtcgct
     9781 tttccgtatg gcgaaaaaag cgaagagttt attgttgagc gagtggtggc aattgtcacc
     9841 gcagtgcaag aacgttatcc ccttgcgctg gctgtggtcg cttgcaacac cgccagtacc
     9901 gtttcacttc ctgcattacg cgaaaagttc gacttcccgg ttgttggtgt cgtgccggcg
     9961 attaaacctg ctgcacgtct gacggcaaat ggcattgtcg gattactggc aacccgcgga
    10021 actgttaaac gttcttatac tcatgagctg atcgcgcgtt tcgctaatga atgccagata
    10081 gaaatgctgg gctcggcaga gatggttgag ttggctgaag cgaagctaca tggcgaagat
    10141 gtttctctgg atgcactaaa acgtatccta cgcccgtggt taagaatgaa agagccgcca
    10201 gataccgttg tattgggttg cacccatttc cctctactac aagaagaact cttacaggtg
    10261 ctgccagagg gaacccggct ggtggattct ggcgcagcga ttgctcgcag aacggcctgg
    10321 ctgttagaac atgaagctcc ggatgcaaaa tctgccgatg cgaatattgc cttttgtatg
    10381 gcaatgacgc cagaagctga acaattattg cccgttttac agcgttacgg cttcgaaacg
    10441 ctcgaaaaac tggcagtttt aggctgattt ggttgaatgt tgcgcggtca gaaaattatt
    10501 ttaaatttcc tcttgtcagg ccggaataac tccctataat gcgccaccac tgacacggaa
    10561 caacggcaaa cacgccgccg ggtcagcggg gttctcctga gaactccgac agagaaagca
    10621 aaaataaatg cttgactctg tagcgggaaa gcgtattatg cacaccccgc gccgctgaga
    10681 aaaagcgaag cgg
//
LOCUS       DBANUU010000149        10471 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000149 DBANUU010000000
VERSION     DBANUU010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.151"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            151..726
                     /gene="ssuE"
                     /locus_tag="ACIE68_003904"
     CDS             151..726
                     /gene="ssuE"
                     /locus_tag="ACIE68_003904"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFX5910149.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNCLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            719..1678
                     /gene="ssuA"
                     /locus_tag="ACIE68_003905"
     CDS             719..1678
                     /gene="ssuA"
                     /locus_tag="ACIE68_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFX5910150.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VTAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1675..2820
                     /gene="ssuD"
                     /locus_tag="ACIE68_003906"
     CDS             1675..2820
                     /gene="ssuD"
                     /locus_tag="ACIE68_003906"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFX5910151.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2831..3622
                     /gene="ssuC"
                     /locus_tag="ACIE68_003907"
     CDS             2831..3622
                     /gene="ssuC"
                     /locus_tag="ACIE68_003907"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFX5910152.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3619..4386
                     /gene="ssuB"
                     /locus_tag="ACIE68_003908"
     CDS             3619..4386
                     /gene="ssuB"
                     /locus_tag="ACIE68_003908"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFX5910153.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4593..7205)
                     /gene="pepN"
                     /locus_tag="ACIE68_003909"
     CDS             complement(4593..7205)
                     /gene="pepN"
                     /locus_tag="ACIE68_003909"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFX5910154.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQCTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7471..8673
                     /gene="pncB"
                     /locus_tag="ACIE68_003910"
     CDS             7471..8673
                     /gene="pncB"
                     /locus_tag="ACIE68_003910"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFX5910155.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYKKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8842..10242
                     /gene="asnS"
                     /locus_tag="ACIE68_003911"
     CDS             8842..10242
                     /gene="asnS"
                     /locus_tag="ACIE68_003911"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFX5910156.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2363 a         2704 c         2813 g         2591 t
ORIGIN      
        1 atatgcgtga tatataaagc cctttcttta gtttattttc agaaaaagat acacaaaatt
       61 tatatttggc attttcttgt ctctccgaca gactgaccga attgttatca atgttaacaa
      121 aaaaagaaca attggttata aggagagagt atgcgtgtca tcaccctggc gggtagtcct
      181 cgctttcctt ctcgctccag ctccttgctg gaatatgcgc gggaaaaact aaattgcctg
      241 gatgtagagg tttatcactg gaatctgcaa aacttcgccc cggaagatct gctttacgct
      301 cgtttcgata gtccggcact caagaccttc accgaacagc tgcaacaggc tgatggactg
      361 atagtcgcta cacctgtgta taaagccgcc tattccggcg cgctgaaaac cctgctcgac
      421 ctgctgccag agcgcgcttt gcaaggcaaa gtggtgctac cgctggcgac gggcggtacg
      481 gtggctcatc tgctggcggt cgattatgcc cttaagccag ttttaagtgc gatgaaagcc
      541 caggagatcc tgcacggcgt gtttgccgat gactcacaag ttattgatta ccatcacaaa
      601 ccccagttca cgccaaatct gcaaacccgt cttgataccg cgctagaaac tttctggcag
      661 gcattgcacc gccgcgatgt tcaggttcct gaccttctgt ctctgcgagg taatgtccat
      721 gcgtaacatc attaaactgg cgctggcggg attgcttagc gtctctactt ttgcggttgc
      781 tgcagaatcc tcgcctgaag cgttacgtat aggctatcag aaaggcagta ttggtatggt
      841 attggcaaaa agccaccagt tactggaaaa acgctatccg caatcaaaaa tctcctgggt
      901 ggagttcccc gcgggtccgc aaatgttgga agcgttaaac gttggcagta ttgatctcgg
      961 cagtaccggg gatattccgc caatctttgc ccaggctgcc ggggctgatt tggtatatgt
     1021 gggcgtcgag ccaccgaagc ccaaagccga agtgattctg gtggcagaaa acagcccgat
     1081 caaaaccgta gccgatctta aaggtcacaa agttgccttt cagaaaggtt ccagttcaca
     1141 caacctttta ctgcgtgcac tgcgtcaggc cggacttaag tttaccgata tccaacccac
     1201 ttacctgacg cccgctgatg cccgcgccgc gttccagcaa ggtaacgttg acgcctgggc
     1261 tatctgggat ccctactact ctgctgcatt attacagggc ggcgtgcggg tgctgaaaga
     1321 cggcaccgat ctcaatcaaa ctggatcgtt ttatctggca gctcgtccct atgcagaaaa
     1381 aaacggcgct tttattcagg gcgtactcgc aacctttagt gaggccgatg cgttaacccg
     1441 cagccagcgc gagcaaagca tcgctttact ggcaaaaacg atgggcttac cggcaccggt
     1501 gattgcctct tacttagatc atcgccctcc taccaccatc aaaccggtta ccgccgaggt
     1561 tgccgcctta cagcagcaaa cggcagatct gttttatgaa aatcgtctgg tgccgaaaaa
     1621 agtcgatatt cgccagcgca tctggcagcc cactcaactg gaaggaaaac aattatgagt
     1681 ctgaatatgt tctggttttt accgacccat ggtgacgggc attatcttgg aacggaagaa
     1741 ggttcacgcc cggttgatca cggctatctg caacaaattg cgcaagcggc ggatcgtctg
     1801 ggctataccg gtgtgctgat cccaacgggg cgatcgtgtg aagatgcgtg gctggtggcg
     1861 gcgtcgatga tcccggtgac gcagcggctg aagtttcttg tcgccctgcg ccccagcgta
     1921 acctcaccta ccgttgccgc tcgccaggcc gccacgcttg accgtctctc aaatggtcgt
     1981 gcgttgttta atctggtcac aggcagcgat ccacaagagc tggcaggcga cggcgtgttc
     2041 cttgatcata gcgagcgcta cgaagcctcg gcggaattta cccaggtctg gcggcgctta
     2101 ttgctggggg aaaccgtcaa tttcaacggt aaacatattc atgtgcgcgg agcaaaactg
     2161 ctcttcccgc cgattcaaca gccttatcct ccgctttact ttggcggatc gtcagacgtc
     2221 gcccaggagc tggcggcaga acaagttgat ctctacctca cctggggcga accgccagaa
     2281 ctagtaaaag agaagatcga acaagtgcgg gcgaaagctg ccgcgcatgg acgcaaaatt
     2341 cgtttcggta ttcgtctgca tgtgattgtt cgtgaaacta acgacgaagc gtggcaggcc
     2401 gccgagcggt taatctcgca tcttgatgat gaaactatcg ccaaagcaca ggccgcattc
     2461 gcccggacgg attccgtagg acaacagcga atggcggcgt tacataacgg caagcgcgac
     2521 aatctggaga tcagccccaa tttatgggcg ggcgttggct tagtgcgcgg cggtgccgga
     2581 acggcgctgg tgggcgatgg tcctacggtc gctgcgcgaa tcaacgaata cgccgcgctt
     2641 ggcatcgaca gctttgtgct ttcgggctat ccgcatctgg aagaagcgta tcgggtcggc
     2701 gagttgctgt tcccacatct ggatgtcgcc attccggaaa ttccccagcc gcagccgctg
     2761 aatccgcaag gcgaagcggt ggcgaatgat tttatccccc gtaaagtcgc gcaaagctaa
     2821 ggaagaaata atggcaacgc cagtgaagaa gtggttattg cgcgttgccc cctggttttt
     2881 accggtgggc atcgtggcgg tgtggcaact ggcctcctca gttggctggc tgtcgacgcg
     2941 tattttgccc tcaccggaag gggtggtgac ggcgttctgg acgctctccg ccagcggcga
     3001 actgtggcag catctggcga tcagttcctg gcgggcgctg attggctttt caattggcgg
     3061 atcgctgggg ttgattttgg gactgattag cgggctatct cgctggggag aacggctgct
     3121 ggatacctca attcagatgt tgcgcaacgt accgcatctg gcgctgatcc cgctggtgat
     3181 tttgtggttt ggcattgatg aatccgcaaa aatctttctg gtggcgctcg gcacgctatt
     3241 ccccatttat atcaacacct ggcatggcat ccgtaatatc gatcgcggtc tggtggaaat
     3301 ggcgcgtagc tatggattat ccggcatacc gctgtttatc catgtgatcc tgcctggtgc
     3361 cctgccctca attatggtcg gcgtgcgttt tgcgttgggc ctgatgtggc tgacgctgat
     3421 tgttgccgaa accatttctg ccaattcagg cattggttat ctggcgatga atgcgcggga
     3481 gtttttgcaa acggacgtgg tggtagtcgc tattattctt tacgccctgc tcggcaaact
     3541 tgctgacgtc agcgcgcagc tgttagagcg cctctggttg cgctggaacc cggcttatca
     3601 tttgaaggag gccacggtat gaatactgct cgtctgaacc agggcacacc attgttgctc
     3661 aatgcagtaa gcaaacatta cgcggaaaat atcgtcctga accaactgga tttacatatt
     3721 ccggcaggtc agtttgtggc ggtggtgggc cgcagcggtg gtggcaaaag taccctgctg
     3781 cgcctgctgg caggtctgga aacgccaacc gcaggcgatg tgttagcggg caccacaccg
     3841 ctggctgaga ttcaggaaga cacgcgaatg atgtttcagg atgcgcgtct gctgccgtgg
     3901 aaatcggtga ttgataacgt tgggttaggc cttaaaggcc agtggcgcga tgccgcgcgt
     3961 cgggcgttgg ctgcagtagg actggagaat cgcgccgggg aatggcctgc cgcactttct
     4021 ggcgggcaga aacagcgtgt ggcgctggca agagcgttga ttcatcgacc gggtttattg
     4081 ttgcttgatg aaccgctcgg ggcgctggac gccttaacgc gactcgagat gcaggatttg
     4141 attgtgtcac tttggcagca gcacggcttt accgtactgt tggtgacgca tgatgtgagc
     4201 gaagcggtag caatggctga ccgggtgttg ttaattgaag agggaaaaat tggtctggat
     4261 ttgacagtgg atatcccccg tccgcgtcga ttagggtcgg tgcgattagc ggagctggaa
     4321 gcggaagtat tgcaacgggt aatgcggcgt gggcattctg aacaactgat tcggcgtcat
     4381 ggttaatatg ccggatgtgg cgcacaaaac cagcattttc aacggactag taggcctgat
     4441 aagacacgtc aagcatcgca tcaggcagtt gggcacggct gccggatgcg gcgtgaacgc
     4501 cttatccagc ctacaaaacc agcaatttca acggagttgt aggcctgata agacgcgtca
     4561 agcgtcgcat caggcatttt ggctcaaaag tatcaagcca gtgctttggt gatcttctcg
     4621 tacagatcgc cagagagatt ttccagccct ttcaactgtt ccagcgccgc gcgcattttc
     4681 tcctgacgtt tggcatcata acgtttcagg cgaatcagcg gttcaatcag acgtgaagct
     4741 acctgcgggt tacggctgtt gaggtcggta agcatttcca ccaggaactg gtaaccgctg
     4801 ccatcttcgg catggaacgc tgccgggttg ctgcccgcaa acgcaccaat caacgaacga
     4861 atacggttcg ggttgctcat ggtaaatgag cgatgctgca acagaccacg caccgtctcc
     4921 agcacattcg ccgccgggct ggtggcttgc aggataaacc atttatccat caccagaccg
     4981 tcctgatgcc acttatcgtc gtactcctgc atcagcgcgt cacggcaagg cagctgtgca
     5041 gcaaccgccg cagaaagcgc tgccagcgca tcggtcatat tgtttgcttc atggtattgc
     5101 ttgctcacca gcacgtcagc cagatgcgtt tcaccaaaag caaggaagcg caggcaggca
     5161 ttacgcagag tgcgttttgc aatatcttca tgctcaacac ggtactcgct ctggtaattc
     5221 gcgttgtaaa tagccagcag ctcatccgcc agttcagtcg ccagagtacg agtgagtgct
     5281 tcgcgtactt cggcaatagc aatcgggtcg atgatatcga acagttcagc catttcattg
     5341 acagaaggca gcgtcaggat ttctgccgcc agcgccggat caatcttctc atcgagcagc
     5401 accgcgcgga aagcgtcagc cacatgtacc ggcagagaca gcggctgccc ttgctgatga
     5461 cgggcgacgt tcagcttgat gtaggttgcc agcaaacttt gcgccgcatc ccaacgggag
     5521 aaatcattac gcgcatgacg catcaggaag gtcagttgct gatcgctcca cttatattcc
     5581 agtttcactg gcgcagagaa ttcgcacagc agcgcaggca ccggctggaa gtagacatta
     5641 tcaaaaacaa aggtctgttc cgcctgggtg acgttcagca cggaattcac cggatgaccg
     5701 cctttctgca acgggatcac tttgccttcg ttgtcataca attcgatggc aaatggaata
     5761 tgcagcggct gtttttctgc ctgatccggc gtggctggcg tgcgctggct gatggtcagg
     5821 gtgcactgct cggtttccgg attgtagtcg tctttgacgg tcacaatcgg cgtgccggac
     5881 tggctgtacc aacggcggaa atgggagaga tcgacattcg acgcatcttc catcgcctgc
     5941 acaaagtcgt cgcaggttgc cgcgctgcca tcatggcgct cgaaatagag ttgcatccct
     6001 ttctggaagt tttcttcgcc cagcagggtg tggatcatgc gaatcacttc cgcgcccttc
     6061 tcgtaaacgg tcagggtgta gaagttgttc atctcaatga ccatatccgg gcggatcggg
     6121 tgcgccatcg ggctggcgtc ttccgcaaac tgcaatccgc gcatggtgcg cacattattg
     6181 atacggttta ccgcgcggga accaaggtca gagctgaatt cctgatcgcg gaagacggtt
     6241 aaaccttctt tcaggctgag ctggaaccag tcgcggcagg tcactcggtt accggtccag
     6301 ttatggaaat attcatggcc gataacgcgt tcaatatcga ggtaatcttt gtcagtggcg
     6361 gtgtcggtgc gggccagcac atatttggag ttaaagatat tcagcccctt attctccatt
     6421 gcgcccatat tgaagaaatc caccgcgacg atcatataga tgtcgaggtc atactccagg
     6481 ccgaagcgtt cttcatccca tttcattgag tttttcagcg aggtcatcgc ccacggcgcg
     6541 cgatcaaggt tgccgcgatc gacgtacagc tccagcgcca cttcgcgacc agaacgcgtg
     6601 gtaaaggtgt cgcgcagtac atcaaagtcg cctgccacca gcgcaaacag gtagcacggt
     6661 ttcgggaacg ggtcctgcca ctgtacccaa tggcgtccgt tttccagttc accttgcgca
     6721 acgcggttac cgttggaaag caggaaggga tatttggttt tatcggcaat aattttggtg
     6781 gtaaaacgcg ccagcacgtc cgggcggtcg agataatacg taatatggcg gaaaccttcg
     6841 gcttcacact gggtgcaaag cgcatcgcct gactgataaa gcccttccag cgccgtattc
     6901 gccgccgggc ttatttcatt aatgatctta agcgtaaaac gctccggcaa attactgatg
     6961 accagtgcgc cctcttcttc tttccaggcg gtccacggct catcattaat atgaacagaa
     7021 accagtttga ggtcttcgcc gttgagacga agcggagcat ctgatgcacc atgacggaca
     7081 gcctggctga ccgcggtaac gaccgtcttt tgcgcgtcga ggtcaaaggt caagtcaata
     7141 tcagtaatct ggtaatccgg cgcacgataa tcgtgacggt atttggcttg tggctgttga
     7201 gtcataaata acctttagca tcttttatag agtctggtgt tcagtctatt cctgttgcgt
     7261 aaatcgcgct atgcagaatc ttcatctttt caggtacaaa cgcctttatt gctacatttt
     7321 tataacatac accgcgtaat gccatcgacc agaaaggtgg catatggtgt gatcggggtt
     7381 caataaattg cgaaacaagg tatactccag cagttcctga agatgtttat tgtactaaac
     7441 gctcctgtac gaggacgcta ctgcgcacct atgacacaat tcgcttctcc tgttctgcac
     7501 tcgttgctgg atacagatgc ttataagttg catatgcagc aagccgtgtt tcatcactat
     7561 tacgatgtgc atgtcgcggc ggagtttcgt tgccgaggtg acgatctgct gggtatttat
     7621 gccgatgcta ttcgtgaaca gattcaggcg atgcagcacc tgcgcctgca ggatgatgaa
     7681 tatcagtggc tctctgccct gcctttcttt aaggccgact atcttaactg gttacgcgag
     7741 ttccgcttta acccggaaca agtcaccgtg tccaacgata atggcaagct ggatattcgc
     7801 ttaagcggtc cgtggcgtga agtcatcctc tgggaagttc ctttgctggc ggttatcagt
     7861 gaaatggtac atcgctatcg ctcaccgcag gccgacgttg cgcaagccct cgacacgctg
     7921 gaaagcaaat tagtcgactt ctcggcgtta accgccggtc ttgatatgtc gcgcttccat
     7981 ctgatggatt ttggcacccg tcgccgtttt tctcgcgaag tacaagaaac catcgttaag
     8041 cgtctgcaac aggaatcctg gttcgtgggc accagcaact acgatctggc gcgtcggctt
     8101 tccctcacgc cgatgggaac acaggcgcac gaatggttcc aggcacatca gcaaatcagc
     8161 ccggacctag ccaacagcca gcgagctgca cttgctgcct ggctggaaga atatcccgac
     8221 caacttggca ttgcattaac cgactgcatc actatggatg ctttcctgcg tgatttcggt
     8281 gtcgagttcg ccagtcggta tcagggcctg cgtcatgact ctggcgaccc ggttgaatgg
     8341 ggtgaaaaag ccattgcaca ttataaaaag ctgggaattg atccacagag taaaacgctg
     8401 gttttctctg acaatctgga tttacgcaaa gcggttgagc tataccgcca cttctcttcc
     8461 cgcgtgcaat taagttttgg tattgggact cgcctgacct gcgatatccc ccaggtaaaa
     8521 cccctgaata ttgtgattaa gttggtagag tgtaacggta aaccggtggc gaaactttct
     8581 gacagccctg gcaaaactat ctgccatgat aaagcgtttg ttcgggcgct gcgcaaagcg
     8641 ttcgaccttc cgcatattaa aaaagccagt taatatcatc agggagctaa tcggctccct
     8701 ttttttacct ttaattccga aatctttcgc tgcatttgcg aattctgctt gtctgattgc
     8761 agatgccagg taacataggt atccccccat ttacgggtgg acaagtgttt attttttccg
     8821 actattaaca gagagaatat tatgagcgtt gtgcctgtag ccgacgtact ccagggccgc
     8881 gtagccgttg acagcgaagt caccgtacgc ggatgggtac gtacccgccg agattcaaaa
     8941 gctggcatct ccttcctcgc cgtttatgac ggttcctgct ttgatcctgt acaggctgtc
     9001 atcaataatt ctctgcccaa ttacaatgaa gacgtcctgc gtctgaccac cggctgctcg
     9061 gtcattgtga cgggtaaagt cgtggcgtcg ccgggccagg ggcaacaatt tgaaattcag
     9121 gccagcaaag ttgaagttgc tggttgggtt gaagatccag acacttaccc gatggcggca
     9181 aaacgccaca gcattgagta tctgcgtgaa gtcgctcacc tgcgtccgcg cacaaacctg
     9241 attggtgccg tcgcgcgcgt tcgccatacg ctggcgcagg cgctgcatcg cttctttaac
     9301 gagcagggat tcttctgggt ttcaacgcca ctgattaccg catctgatac cgaaggtgca
     9361 ggcgaaatgt tccgcgtttc tacgctggat ctggaaaacc tgccgcgtaa cgatcagggc
     9421 aaagtggatt tcgacaaaga cttctttggt aaagagtctt tcctgaccgt atctggccag
     9481 ttgaacggcg aaacctacgc ttgcgcatta tccaaaattt ataccttcgg cccgactttc
     9541 cgtgctgaaa actccaacac cagccgtcac ctggcggaat tctggatgct ggagccggaa
     9601 gtggcgtttg ctaacctgaa cgatatcgcg ggtctggctg aagccatgct gaaatatgtc
     9661 ttcaaagcgg ttctcgaaga acgcgcagac gacatgaaat tcttcgctga acgcgtagat
     9721 aaagatgccg tttcacgtct ggaacgcttc attgaggccg atttcgcgca ggtggattac
     9781 accgacgcag tgaccattct cgaaaactgc ggcaggaagt ttgaaaaccc ggtttactgg
     9841 ggcgtcgatc tctcttctga gcatgagcgt tatctggcgg aagaacactt taaagcaccg
     9901 gtagtggtta aaaactatcc gaaagatatt aaagcgttct atatgcgcct caacgaagac
     9961 ggtaaaaccg ttgcggctat ggacgttctg gctccgggca tcggtgagat cattggtggc
    10021 tcccagcgtg aagaacgtct ggacgttctg gacgagcgta tgctggaaat gggcctgaat
    10081 aaagaagatt actggtggta tcgcgatctg cgtcgctacg gtactgttcc gcattcaggt
    10141 ttcggtcttg gttttgaacg cctgattgct tacgtaactg gcgtgcaaaa cgtgcgtgat
    10201 gtgattccgt tcccacgtac tccgcgcaac gccagcttct gattttcttc ctcaaagagc
    10261 cagcgcctgc tggctctttt ttatcctttc tgtcaagtta tctgtttgtt aagtcaagca
    10321 atctatttgc aaccccgcca taacgatcat cctgttacgg aatattacat tgcaacattt
    10381 acgcacaaaa aataacccgc attcttattg cggattagtt ttttcttagc tgatagcaca
    10441 attttcatac tattttttgg cattctggat g
//
LOCUS       DBANUU010000150        10438 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000150 DBANUU010000000
VERSION     DBANUU010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.152"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(13..3654)
                     /locus_tag="ACIE68_003912"
     CDS             complement(13..3654)
                     /locus_tag="ACIE68_003912"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFX5910157.1"
                     /translation="MRIVIDLQGAQTESRFRGIGRYSIAIARGIIRNNSRHEIFIALS
                     AMLDESIADIKAQFADLLPAENIVVWHAVGPVRAMDQGNEWRRESAELIREAFLESLC
                     PDVVFITSLFEGHVDDAATSVHKFSRQYKVAVLHHDLIPLVQAETYLQDDVYKPYYLQ
                     KVEWLKNADLLLTNSAYTAQEAIEHLHLQGDHVQNIAAAVDSQFCMAEVAASEKETVL
                     GHYGIQREFMLYAPGGFDSRKNFKRLIEAYAGLSDALRRSHQLVIVSKLSIGDRQYLE
                     SLASGNGLQQGELVLTGYVPEDELIQLYRLCKLFIFASLHEGFGLPVLEAMSCGAPVI
                     GSNVTSIPEVIGNPEALFDPYSVSSMRDKIAQCLTDDTFLARLKEMAQQQARNFSWDK
                     AAVTALEAFEKIAVEDTGTAQVLPEALIQKILAISQGQPDDRDLRLCATAIDYNLKTA
                     ELYQIDDKSLNWRVEGPFDSSYSLALVNREFARALSADGVEVLLHSTEGPGDFAPDAS
                     FMAQSENSDLLAFYNQCQTRKSNEKIDIISRNIYPPRVTKMDAKVKFLHCYAWEETGF
                     PQPWINEFNRELDGVLCTSEHVRKILIDNGLNVPAFVVGNGCDHWLNIPAETTKDVDH
                     GTFRFLHVSSCFPRKGIQAMLQAWGKAFTRRDNVILIIKTFNNPHNEIDAWLAQAQAQ
                     FIDYPKVEVIKEDMSATELKGLYESCDVLVAPGCAEGFGLPIAEAMLSGLPAIVTNWS
                     GQLDFVNSQNSWLVDYQFTRVKTHFGLFSSAWASVDIDNLTDALKAAASTDKSVLRDM
                     ANAGRELLLQQFTWKAVADRSCQAVKTLRAHIDIAQHRARIGWVTTWNTKCGIATYSQ
                     HLVESAPHGADVVFAPQVSAGDLVCADEEFVLRNWIVGKESNYLENLQPHIDALRLDV
                     IVIQFNYGFFNHRELSAFIRRQHDAGRSVVMTMHSTVDPLEKEPSWNFRLSEMKEALA
                     LCDRLLVHSIADMNRLKDLGLTANVALFPHGVINYSAASVTRQQQSLPLIASYGFCLP
                     HKGLMELVESVHRLKQAGKPVRLRLVNAEYPVGESRDLVAELKAAAQRLGVTDLIEMH
                     NDFLPDAESLRLLSEADLLIFAYQNTGESASGAVRYGMATQKPVAVTPLAIFDDLDDA
                     VFKFDGCSVDDISQGIDRILNSIREQNSWATRTQQRADAWREQHDYQAVSRRLVNMCQ
                     GLAKAKYFK"
     gene            complement(3657..4934)
                     /locus_tag="ACIE68_003913"
     CDS             complement(3657..4934)
                     /locus_tag="ACIE68_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFX5910158.1"
                     /translation="MGSSFYRSFEERHRGSVEEIKRRLSFYLPFLAGLKDIYPDGVIA
                     DIGCGRGEWLEILTENGIANIGVDLDDGMLARAREAGLNVQKMDCLQFLQSQADQSLI
                     ALTGFHIAEHLPFEVLQQLAMHTLRVLKPGGLLILETPNPENVSVGTCSFYMDPTHNH
                     PLPPPLLEFLPIHYGFTRAITVRLQEKEVLQSPDAAVNLVDVLKGVSPDYSIIAQKAA
                     PTDILERFDTLFTQQYGLTLDALSNRYDAILRQQFSSVVSRLETLNQTYMQQISQMSE
                     TIQTLQGEVDDLSHVIDQNHQLHQQMADLHNSRSWRITQPLRWLSLQRQLLRQEGAKV
                     RARRAGKKILRKGMALSLVFFHRYPKSKVYLFKVLRKTGCYTLLQRLFQRVMLVQSDT
                     MMMQSRRYDVGTEEMTSRAMSIYNELKNKNTEK"
     gene            complement(4934..6148)
                     /gene="wzt"
                     /locus_tag="ACIE68_003914"
     CDS             complement(4934..6148)
                     /gene="wzt"
                     /locus_tag="ACIE68_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O8 family O-antigen export ABC transporter
                     ATP-binding subunit"
                     /protein_id="HFX5910159.1"
                     /translation="MSYIRVNNVGKAYRQYHSKTGRLIEWLSPLNTKRHNLKWILRDI
                     NFEVAPGEAVGIIGINGAGKSTLLKLITGTSRPTTGEIEISGRVAALLELGMGFHSDF
                     TGRQNVYMSGQLLGLSSEKITELMPQIEEFAEIGDYIDQPVRVYSSGMQVRLAFSVAT
                     AIRPDVLIIDEALSVGDAYFQHKSFERIRKFRQEGTTLLLVSHDKQAIQSICDRAILL
                     NKGQIEMEGEPEAVMDYYNALLADKQNQSIKQVEHNGKTQTVSGTGEVTISEVHLLDE
                     QGNVTEFVSVGHRVSLQVNVEVKDDIPELVVGYMIKDRLGQPIFGTNTYHLNQTLTSL
                     KKGEKRSFLFSFDARLGVGSYSVAVALHTSSTHLGKNYEWRDLAVVFNVVNTEQQEFV
                     GVSWLPPELEIS"
     gene            complement(6148..6942)
                     /gene="wzm"
                     /locus_tag="ACIE68_003915"
     CDS             complement(6148..6942)
                     /gene="wzm"
                     /locus_tag="ACIE68_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O8 family O-antigen export ABC transporter
                     permease subunit"
                     /protein_id="HFX5910160.1"
                     /translation="MRDLLTTIYRYRGFIWSSVKRDFQARYQTSMLGALWLVLQPLSM
                     ILVYTLVFSEVMKARMPDNTGSFAYSIYLCSGVLTWGLFTEMLDKGQSVFINNANLIK
                     KLSFPKICLPIIVTLSAVLNFAIIFSLFLIFIIVTGNFPGWLFLSVIPVLLLQILFAG
                     GLGMILGVMNVFFRDVGQLVGVALQFWFWFTPIVYVLNSLPAWAKNLMMYNPMTRIMQ
                     SYQSIFAYHLAPNWYSLWPVLALAIIFCVIGFRMFRKHAADMVDEL"
     gene            complement(6944..8320)
                     /gene="rfbK"
                     /locus_tag="ACIE68_003916"
     CDS             complement(6944..8320)
                     /gene="rfbK"
                     /locus_tag="ACIE68_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003596869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O9 family phosphomannomutase RfbK"
                     /protein_id="HFX5910161.1"
                     /translation="MTQLTCFKAYDIRGELGEELNEDIAYRIGRAYGEFLKPGKIVVG
                     GDVRLTSESLKLALARGLMDAGTDVLDIGLSGTEEIYFATFHLGVDGGIEVTASHNPM
                     NYNGMKLVRENAKPISGDTGLRDIQRLAEENQFPPVDPARRGTLSKISVLKEYVDHLM
                     SYVDFSNFTRPLKLVVNSGNGAAGHVIDEVEKRFAAAGVPVTFIKVHHQPDGHFPNGI
                     PNPLLPECRQDTADAVREHQADMGIAFDGDFDRCFLFDDEASFIEGYYIVGLLAEAFL
                     QKQPGAKIIHDPRLTWNTVDIVTRNGGQPVMSKTGHAFIKERMRQEDAIYGGEMSAHH
                     YFRDFAYCDSGMIPWLLVAELLCLKNSSLKSLVADRQKAFPASGEINRKLSNAAEAIA
                     RIRAQYEPAAAHIDTTDGISIEYPEWRFNLRTSNTEPVVRLNVESRADVALMNEKTTE
                     LLHLLSGE"
     gene            complement(8344..9759)
                     /locus_tag="ACIE68_003917"
     CDS             complement(8344..9759)
                     /locus_tag="ACIE68_003917"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HFX5910162.1"
                     /translation="MLLPVIMAGGTGSRLWPMSRELYPKQFLRLFGQNSMLQETITRL
                     SGLEIHEPMVICNEEHRFLVAEQLRQLNKLSNNIILEPVGRNTAPAIALAALQATRDG
                     DDPLMLVLAADHIINNQSAFHDAIRVAEQYADEGHLVTFGIVPNAPETGYGYIQRGVA
                     LTDSAHSAYQVARFVEKPDRERAEAYLASGEYYWNSGMFMFRAKKYLIELAKYRPDIL
                     EACQAAVNAADNGSDFINIPHDIFCECPDESVDYAVMEKTADAVVVGLDADWSDVGSW
                     SALWEVSPKDEQGNVLSGDAWVHNSENCYINSDEKLVAAIGVENLVIVSTKDAVLVMN
                     RERSQDVKKAVEFLKQNQRSEYKRHREIYRPWGRCDVVVQTPRFNVNRITVKPGGAFS
                     MQMHHHRAEHWVILAGTGQVTVNGKQFLLSENQSTFIPIGAEHCLENPGCIPLEVLEI
                     QSGAYLGEDDIIRIKDQYGRC"
     gene            complement(10041..10325)
                     /locus_tag="ACIE68_003918"
     CDS             complement(10041..10325)
                     /locus_tag="ACIE68_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910163.1"
                     /translation="MSRLSHQSQHNAVVVCKELLSPNARCLPGGEYDVSSYLSPYRKC
                     PFLIVAGIGKSLDSFFLMAVRACVLPRVNEKVLMVTLAANVEIVGRFVAW"
BASE COUNT         2707 a         2806 c         2433 g         2492 t
ORIGIN      
        1 agagagatat ttttatttaa aatatttagc tttagctaag ccttgacaca tattaaccag
       61 acggcgtgaa acagcttgat aatcatgttg ttcccgccat gcatcggcac gttgttgagt
      121 cctggttgcc caagagttct gttcacggat ggaattcagg atccggtcaa tcccctgact
      181 gatatcatcg acgctgcatc catcaaattt aaagacggca tcgtccaaat catcaaatat
      241 cgccaggggc gttaccgcaa caggtttttg agtcgccata ccataacgta ccgccccgct
      301 agcagactcc ccagtattct gataagcaaa aatcagaaga tcggcttctg aaagcaaccg
      361 cagactctcc gcatcaggta ggaaatcatt atgcatctca atcagatcgg taacacctaa
      421 ccgctgagca gcagctttaa gctctgccac cagatcgcgt gactccccaa caggatactc
      481 tgcgttcacc agtcgtaaac gaaccggttt accggcttgc ttgagtctat ggacggattc
      541 tactagttcc atcaggccct tatgcggtaa gcagaagcca tagctcgcaa ttagcggtaa
      601 agactgctgt tgacgtgtga cgctcgctgc ggagtagttg ataacaccgt gcgggaataa
      661 agcaacattc gcagttaagc ctaaatcttt aaggcggttc atatcggcaa tcgaatgcac
      721 caacaaccgg tcgcaaagtg ccagcgcctc tttcatttca gaaagacgga aattccagct
      781 cggctctttt tccagcggat ccacagttga gtgcatcgtc ataacaactg aacgaccggc
      841 gtcatgctgg cgacgaataa acgccgacag ttctcgatga ttaaagaatc catagttgaa
      901 ttggatcaca atgacatcga gtctcagagc atcaatgtgt ggctggaggt tttccagata
      961 gttgctctct ttacctacaa tccagttgcg aagtacaaac tcttcgtctg cacacacaag
     1021 atcgccagcg ctgacctggg gagcaaaaac aacatccgcg ccatgaggtg cgctttccac
     1081 cagatgctgg gaataggttg cgatcccaca tttcgtgttc caggtcgtca cccagccaat
     1141 gcgcgcccga tgctgtgcaa tatcaatatg cgcacgcaga gtcttgaccg cctggcaaga
     1201 acgatcagcc accgctttcc aggtaaactg ctgcagaaga agctcgcgac cagcattggc
     1261 catgtcacgc agcactgatt tatcggttga ggctgccgct tttaatgcat ctgttaagtt
     1321 gtcaatatcc acactggccc aggctgagga aaacagacca aagtgcgttt ttacccgagt
     1381 gaactgatag tcaaccagcc atgaattttg tgaattaaca aaatcaagtt gcccgctcca
     1441 attggtgacg atagccggta gcccactcag cattgcttca gcaataggta aaccaaagcc
     1501 ttcagcgcaa cctggagcaa ccaaaacatc acagctttca taaagccctt taagctcggt
     1561 ggctgacata tcctctttga tcacttcaac tttgggatag tctatgaatt gagcctgggc
     1621 ctgagccagc catgcgtcaa tttcattgtg cggattgtta aaagtcttaa tgattaagat
     1681 aacattgtca cgacgagtga acgccttccc ccaagcctga agcattgcct gtatcccttt
     1741 gcgtgggaaa caagaagaga cgtgcaggaa acggaatgtt ccgtgatcca catcttttgt
     1801 cgtctcggct gggatattga gccaatggtc acagccattg ccaacaacaa atgcgggcac
     1861 attcagtccg ttatcaatca gtattttacg aacatgttcc gaagtacaca gcactccgtc
     1921 aagttcccga ttaaattcat tgatccacgg ttgcggaaag cccgtttctt cccaagcata
     1981 acaatgaagg aattttactt tggcatccat tttggtaacc cgcggtggat agatatttct
     2041 gctaataata tctatctttt cgttactctt gcgggtctga cattgattat aaaatgccag
     2101 aagatcacta ttttccgact gtgccataaa cgaggcatct ggggcaaaat cacctggtcc
     2161 ttcagtggaa tgcaataaaa cctctacacc atcggctgag agtgcccggg caaattcgcg
     2221 gttgaccaac gccagactat atgagctatc gaatgggcct tccacacgcc agttcagcga
     2281 tttatcgtcg atttgataaa gttctgccgt tttcagattg taatcaatgg ccgttgcgca
     2341 caagcgcaga tcgcggtcat ctggctgccc ttgtgagata gcaaggatct tctgaatcaa
     2401 agcttcaggc aaaacctgcg cagtaccggt gtcttctacc gcgatctttt cgaaagcttc
     2461 cagagcagtc accgcagctt tatcccaaga gaaattacgc gcttgctgct gcgccatttc
     2521 tttcagacgc gcgaggaagg tatcatcagt caaacattgc gcgatcttat ccctcatgga
     2581 agagacagaa tacgggtcga ataatgcctc aggattaccg atgacttcag gaatactggt
     2641 gacatttgag ccaatcaccg gcgcaccgca cgacattgct tccagaaccg gcaacccaaa
     2701 accttcatgt agtgaagcaa agatgaacag cttacatagg cgatagagct ggatcagctc
     2761 atcttccggc acataaccag tgagtaccag ttcgccctgc tgtaaaccat tacctgacgc
     2821 aagggattcc agatactgac gatcaccgat ggaaagctta ctgacgatga ccagttgatg
     2881 actgcgacgt aaggcatcac tgagcccggc ataggcctca atcaaccgtt taaagttttt
     2941 ccttgagtca aatcctccgg gcgcatacaa catgaactcg cgctgaatac cgtaatggcc
     3001 aaggacggtc tctttttcgc tcgctgccac ctccgccata caaaactgag aatcgactgc
     3061 ggctgcaata ttctgcacat gatcgccctg taaatgcaga tgctcgatcg cttcctgtgc
     3121 ggtataagca gagttagtca acaaaaggtc agcgtttttt aaccactcaa ctttctgtaa
     3181 atagtagggt ttgtatacat cgtcctgcag ataggtttcc gcctgcacga gggggataag
     3241 atcgtggtgc agtacggcta ctttatactg acgactaaat ttgtgtaccg atgtagccgc
     3301 atcgtcgaca tgaccttcaa acaaactcgt aatgaaaacg acatctggac acaatgattc
     3361 aagaaacgct tcccgaatca gttctgcgct ctcccgacgc cattcattac cttggtccat
     3421 cgcacgaaca gggcctacgg catgccatac gactatattt tctgccggca ggagatcggc
     3481 aaattgcgcc ttaatatctg caatcgactc atccagcatg gcggatagcg cgatgaaaat
     3541 ctcatgccgg ctgttatttc tgattatgcc tctggcgatt gcgatactat aacgaccgat
     3601 gccacgaaag cggctttccg tctgtgcgcc ttgtaaatct atgacaatac gcatcgttat
     3661 ttctccgtat ttttattttt taattcgtta taaatactca tcgcgcgact tgtcatttct
     3721 tcagtaccca catcatatct tctggactgc atcatcatcg tgtcagattg caccagcatt
     3781 acgcgttgga acaaacgttg tagcaatgta tagcagccag tttttctcag aaccttaaac
     3841 agataaacct tagacttagg gtaacgatgg aaaaagacca gcgagagcgc catccctttg
     3901 cgcaatattt ttttcccagc cctacgggct cgcactttag ccccttcctg acgtaataat
     3961 tgacgttgca aagacaacca gcgtagtggt tgagtaatac gccatgaacg actgttatgt
     4021 aaatccgcca tttgctgatg aagctgatgg ttctgatcga tgacatgact cagatcgtca
     4081 acctcacctt gcaacgtctg aatagtctct gacatttggc ttatctgttg catataggtt
     4141 tggttcaacg tctccagccg tgagacaacg gacgaaaact gttggcgcaa aatcgcatcg
     4201 taacggttgc tcagagcatc cagcgtcaga ccgtactgct gggtaaacag ggtgtcaaag
     4261 cgttcaagaa tatctgttgg cgctgctttc tgagcaatga tgctgtagtc ggggctcacc
     4321 cctttgagta catcgaccaa attaacggct gcatccggag attgaagaac ctctttttcc
     4381 tgcagacgaa cggtaattgc tcgggtaaaa ccataatgaa taggtaaaaa ctcaagcagt
     4441 ggcggtggca gaggatgatt atgcgttgga tccatataaa atgaacaggt gccgacgctt
     4501 acattctccg ggttcggcgt ttcgaggatc agcaaaccac ctggtttcag cacccgtagg
     4561 gtatgcatgg cgagttgctg caggacctca aacggcaaat gctcagcaat atgaaaaccg
     4621 gtcaacgcta tcaggctctg atccgcctga ctttgcaaaa actgcagaca atccattttc
     4681 tgcacattca gtccggcctc cctggcgcgc gccagcatgc catcatcgag atcgacgccg
     4741 atgttcgcaa tgccattttc agtcaggatc tccaaccatt cgccacgtcc gcaaccaata
     4801 tccgcaatca cgccatcagg ataaatgtcc ttcagacctg caagaaaagg taaataaaaa
     4861 ctcaagcggc gcttgatttc ttcaaccgaa cctctgtgtc gttcttcaaa tgaacgataa
     4921 aacgacgaac ccattaagaa atctccagtt caggcggcaa ccaggacacg ccgacaaact
     4981 cttgttgttc cgtgttaacg acgttgaata ccacggccag atcgcgccat tcatagtttt
     5041 tgccgaggtg cgtactggaa gtatgcaacg cgacagcgac agaataggag ccaaccccca
     5101 atctcgcatc gaaagaaaat aagaacgaac gcttttctcc ttttttcagg gaggtgagtg
     5161 tctgattgag atggtacgta ttggtcccga aaatcggctg cccaagtcga tccttaatca
     5221 tatatccgac aacaagctca ggaatatcgt ccttgacctc aacgttgacc tgcaagctga
     5281 cacgatgccc taccgaaaca aattcagtca cattgccctg ttcatcgaga agatgaacct
     5341 cagagatagt cacctcacca gtgcctgaaa cagtttgcgt tttaccatta tgctcaactt
     5401 gtttaatgga ctgattttgt ttatcggcca gaagagcatt gtaataatcc atcactgctt
     5461 caggttcacc ttccatttca atttggcctt tattcaataa aatggcccgg tcgcaaatgc
     5521 tttggatcgc ttgtttatca tgggatacca gcaacagcgt ggtcccttcc tgacgaaatt
     5581 ttcgaatacg ctcaaagctt ttatgctgga aatatgcatc cccaacagat aatgcctcat
     5641 cgataattag cacatcagga cggatagccg tcgctacact aaaagctaat cgaacttgca
     5701 tcccactgga gtagacgcgc acaggttgat cgatatagtc cccaatctca gcaaactctt
     5761 caatttgcgg catcagttca gttattttct ctgacgataa ccccaacagt tgcccagaca
     5821 tataaacatt ctgccgacca gtgaaatcag aatgaaaccc catccccaat tcgagtaatg
     5881 cagcgacacg tccggagatt tcaatttctc cagtcgtcgg cctggacgtc ccggttatga
     5941 gtttaagcag ggtactcttg cctgcaccgt tgataccgat aataccgaca gcctcgcccg
     6001 gagcgacttc gaaattaata tcgcggagga tccatttcaa attatggcgt ttggtattca
     6061 gaggggataa ccattcgatc agtctcccgg tctttgagtg atactggcga tacgccttac
     6121 cgacattatt tactctgata taactcatta taattcatcc accatatccg ccgcatgctt
     6181 gcggaacatc ctgaaaccga tgacgcagaa aataatggcg agagccaata ctggccatag
     6241 cgaataccag ttgggggcca gatgataggc gaagatggac tgataagatt gcatgatccg
     6301 agtcatcggg ttatacatca tcagattttt tgcccatgca ggtaatgaat tcagtacata
     6361 aacaatgggt gtgaaccaaa accagaattg cagcgcaacg ccaaccagtt gccccacatc
     6421 cctgaaaaag acgttcatga caccaaggat catccccagc ccaccggcaa acaggatctg
     6481 caaaagcagg accggtatca ccgagagaaa gagccagccg gggaagttac cggtgacaat
     6541 gataaaaatt agaaacagac tgaaaataat cgcgaaattt agcaccgccg ataacgtcac
     6601 gatgatcggc agacagattt tcggaaaact gagtttcttg atcagattag cattgttaat
     6661 aaatacgctc tgacctttat ccagcatctc agtaaataat ccccaggtca gtaccccgga
     6721 acagagataa atactatagg caaacgaccc ggtattatcg ggcattcttg ccttcatcac
     6781 ctcggaaaaa accagggtat agaccagaat catagagagc ggttgtaaaa cgagccatag
     6841 tgcgcccagc atactagttt gatagcgtgc ctgaaaatca cgtttaacac tgctccagat
     6901 aaatccccga taacgataaa tcgtcgttag taaatctctc accttattcc ccgcttaaca
     6961 ggtgtaacag ctcggtcgtt ttttcattca taagcgccac atcagctctg gactcaacgt
     7021 tcagacgcac caccggctcg gtgttagacg tgcgcaggtt aaagcgccat tcagggtatt
     7081 caatactgat cccgtccgtt gtgtcgatgt gtgcagccgc cggctcatac tgcgcccgga
     7141 tgcgggcgat cgcctcagca gcattactta gcttgcggtt gatctctccc gacgcaggga
     7201 acgccttctg gcggtccgcc accagcgatt tcagcgagct gttcttcaga cacagcagct
     7261 ccgccaccag cagccacggg atcatcccgc tatcgcagta ggcgaaatcg cggaagtaat
     7321 ggtgcgcact catctccccg ccgtagatag cgtcttcctg acgcatccgc tccttgatga
     7381 acgcatgccc cgtcttcgac atcaccggct ggccgccgtt gcgggtcacg atgtctaccg
     7441 tgttccacgt caagcgcggg tcgtgaatga ttttcgctcc cggctgcttc tgcaggaacg
     7501 cctcagccag caggccgaca atgtaatacc cctcgataaa cgaagcttcg tcatcgaaca
     7561 ggaagcagcg atcgaagtcg ccgtcaaagg caatccccat gtcggcctga tgctcgcgca
     7621 ccgcgtcggc ggtatcctgg cggcactccg gcagcagcgg attcgggata ccgttaggga
     7681 aatggccatc cggctggtga tgcaccttga taaaggttac cggcacccca gccgccgcga
     7741 agcgtttctc cacctcatca atcacgtgcc ccgcagcccc gtttccggag ttcaccacca
     7801 acttcagtgg acgggtgaag ttcgagaagt ccacgtagct catcagatgg tcaacatact
     7861 ccttcagtac cgatatcttg ctcagggtcc cgcgacgcgc cgggtccact ggcgggaact
     7921 ggttttcctc cgccaggcgc tggatatccc gcaggccggt gtcgccgctg atgggcttcg
     7981 cattctcgcg caccagcttc atgccgttgt agttcatagg attgtggctc gcggtcacct
     8041 cgatgccacc atctacccca aggtggaagg tggcaaagta aatctcttcg gtaccgctca
     8101 ggccgatgtc cagcacgtcg gtaccggcgt ccattaaccc gcgggccagc gccagcttca
     8161 gcgactcgct tgtgaggcgc acatcgcccc ccaccactat cttcccgggt ttcagaaatt
     8221 cgccgtaggc gcgaccgata cggtaggcga tgtcctcgtt cagttcctca cccagttcac
     8281 cacggatgtc ataagcttta aaacaagtta actgtgtcat tctgcgtctt gtcccgaaaa
     8341 taattagcaa cgaccatact ggtctttaat acgaataatg tcgtcctcgc caaggtacgc
     8401 ccccgactgg atctccagca cttccagcgg aatacagcca gggttttcca ggcagtgctc
     8461 ggcgccaatc ggaataaagg tggactggtt ctcggacaac aggaactgct taccgttgac
     8521 agtcacctga ccggtgccgg cgagaataac ccaatgctcg gcgcgatggt ggtgcatctg
     8581 catcgagaag gcaccgcctg gtttcaccgt gatgcggttg acgttgaagc gcggggtctg
     8641 gaccactacg tcgcaacggc cccaggggcg gtaaatctca cggtggcgct tgtactcgct
     8701 gcgctggttc tgcttgagga actcgaccgc cttcttcacg tcctgggaac gctcgcgatt
     8761 catcaccagc acggcgtcct tagtgctgac aatcaccaga ttctctacgc caatggccgc
     8821 cactagcttc tcgtcgctgt tgatgtagca gttttcgctg ttgtgtaccc acgcgtcacc
     8881 gctgaggaca ttgccctgct cgtctttcgg gctgacctcc catagtgcgg accaggagcc
     8941 gacgtcgctc cagtcagcat cgagaccgac caccaccgca tcggcggttt tctccataac
     9001 ggcatagtcc acggactcat ccgggcactc gcagaaaata tcatgcggga tattgatgaa
     9061 atcgctgcca ttatcggcgg cattcaccgc agcctggcag gcttccagga tatccggacg
     9121 gtatttggcc agctcgatga ggtatttctt ggcgcggaac ataaacatgc cgctgttcca
     9181 gtagtactcc ccggaggcga ggtaagcctc ggcgcgctcg cgatccggct tctccacaaa
     9241 gcgggccacc tggtacgcgg aatgggcact atcggtgagc gccacgccgc gctgaatgta
     9301 accgtagcca gtttccgggg cattcggcac gataccgaag gtgaccagat gaccttcatc
     9361 agcatactgc tcggcgaccc ggatggcgtc gtggaaggcc gactggttat tgatgatatg
     9421 gtcagcggcg agaaccagca tcagcgggtc gtcgccgtcg cgggtggcct gaagggctgc
     9481 cagggcgatg gccggggcgg tgttgcgccc gaccggctca agaataatgt tattcgacag
     9541 cttattgagc tggcgtagct gttcagccac caggaagcgg tgctcttcgt tacagatgac
     9601 catcggttca tggatttcaa ggcccgagag tcgggtgatg gtttcctgca gcatggagtt
     9661 ctgcccgaac aggcggagga actgtttcgg ataaagctcg cgtgacatcg gccagagacg
     9721 actgccggta ccaccagcca taattacagg aagtaacata atgaaatcct taaataaata
     9781 attgttttat tttactatca atcaatcgac caacaggttg aatggccttt ttcaggtacg
     9841 ctaccgccat tggctatcgc aaccaaaatg cgtagtgctc ccgtgctcca ggctaaacac
     9901 caccctcttt cgcttcagac ggtcggcaga tagttcacgc agcggtctga aaaaccatcc
     9961 ttacggaacg ggtatcctga aaacctaaaa gcagagatat gccctgaggc atgtctctgc
    10021 ttttaatatg tatctgttcc tcaccaggcc acgaaacgcc ccacaatttc cacatttgcc
    10081 gccagagtaa ccatcaggac cttctcattt acgcggggca aaacacatgc tctcaccgcc
    10141 atcaaaaaaa acgaatcaag tgatttaccg atgccagcaa caatcagaaa ggggcattta
    10201 cgatacgggg aaagataaga agagacatcg tattcgccgc ccggcaggca acgcgcgttc
    10261 gggcttaata gctctttgca cacgacgacg gcgttatgtt gcgactggtg ggataaacga
    10321 ctcatgcctc tacactgcac attccacaaa aaaaatcttc gcagattata gcgttggatg
    10381 ctgggaaaaa aaatataaaa gccgactaat taatggatta ctcaaataat acacatct
//
LOCUS       DBANUU010000151         9988 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000151 DBANUU010000000
VERSION     DBANUU010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.153"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            410..1387
                     /gene="glaH"
                     /locus_tag="ACIE68_003919"
     CDS             410..1387
                     /gene="glaH"
                     /locus_tag="ACIE68_003919"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFX5910164.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1407..2675
                     /gene="lhgO"
                     /locus_tag="ACIE68_003920"
     CDS             1407..2675
                     /gene="lhgO"
                     /locus_tag="ACIE68_003920"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFX5910165.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAMELREREPNITGLGGIFVPSSGIVSYRNVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2698..4146
                     /gene="gabD"
                     /locus_tag="ACIE68_003921"
     CDS             2698..4146
                     /gene="gabD"
                     /locus_tag="ACIE68_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFX5910166.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSAPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALSSKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLENGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4160..5440
                     /gene="gabT"
                     /locus_tag="ACIE68_003922"
     CDS             4160..5440
                     /gene="gabT"
                     /locus_tag="ACIE68_003922"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFX5910167.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGNFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            5678..7078
                     /gene="gabP"
                     /locus_tag="ACIE68_003923"
     CDS             5678..7078
                     /gene="gabP"
                     /locus_tag="ACIE68_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFX5910168.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7099..7761
                     /gene="csiR"
                     /locus_tag="ACIE68_003924"
     CDS             7099..7761
                     /gene="csiR"
                     /locus_tag="ACIE68_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFX5910169.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7762..8211)
                     /gene="kbp"
                     /locus_tag="ACIE68_003925"
     CDS             complement(7762..8211)
                     /gene="kbp"
                     /locus_tag="ACIE68_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFX5910170.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8295..8453)
                     /gene="yqaE"
                     /locus_tag="ACIE68_003926"
     CDS             complement(8295..8453)
                     /gene="yqaE"
                     /locus_tag="ACIE68_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFX5910171.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8636..8935
                     /gene="ygaV"
                     /locus_tag="ACIE68_003927"
     CDS             8636..8935
                     /gene="ygaV"
                     /locus_tag="ACIE68_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFX5910172.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8945..9469
                     /gene="ygaP"
                     /locus_tag="ACIE68_003928"
     CDS             8945..9469
                     /gene="ygaP"
                     /locus_tag="ACIE68_003928"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFX5910173.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRHEQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPFPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9516..9920)
                     /gene="stpA"
                     /locus_tag="ACIE68_003929"
     CDS             complement(9516..9920)
                     /gene="stpA"
                     /locus_tag="ACIE68_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFX5910174.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2293 a         2562 c         2719 g         2414 t
ORIGIN      
        1 atatcaatat tcctgggcgt agcgctcttc ttttagggaa aacaggagaa ccgctgaact
       61 atctctattt ataatttaat gttatatctg ccccgataaa acggggcaga taatatgttt
      121 agtttactaa cggtcatttt gcagtgaagc catttactgt tttttatcga ccagataatc
      181 tgttctctaa tgttaactct ccctaacctg ttgctttagt tattcatttc ctgtctcact
      241 ttgccttaat accctacgtt aaatgttact aatttattgc ttttgatcac aataagaaaa
      301 caatatgtcg cttttgtgcg catttttcag aaatgtagat atttttagat tatggctacg
      361 aaatgagcat cgccatgtca ccctacatct cataagagga tcgcttctga tgaatgcact
      421 gaccgccgta caaaataacg ctgtcgattc aggccaggac tatagcggat tcactctcat
      481 cccgtcggcg caatccccgc gtctgctgga actcaccttc accgaacaga cgaccaaaca
      541 gtttctcgaa caggttgccg aatggcccgt gcaggcgctg gagtacaaat cgtttctgcg
      601 ttttcgggta ggcaaaattc ttgacgatct gtgtgcgaat cagctgcaac cattgctgtt
      661 gaagaccctg ctaaaccgcg ctgaaggtgc gctgttgatc aatgcggtgg gtatcgatga
      721 tgtggcgcag gcagatgaga tggtgaagct ggcaacggcg gtagcgcatc tgattggtcg
      781 ctcaaatttc gatgcgatga gcggtcagta ttacgcgcgt ttcgtggtga aaaatgtcga
      841 taactcagac agctatctgc gtcagccgca ccgcgtgatg gaactgcaca acgacggcac
      901 gtatgtcgaa gagatcaccg attacgtgct gatgatgaaa atcgatgagc aaaacatgca
      961 gggtggaaat tcgttgctgc tgcatctcga tgactgggaa catctggacc actatttccg
     1021 ccatccactg gcgcgtcgcc cgatgcgctt tgccgcgccg ccgagcaaaa acgtcagcaa
     1081 agatgttttc catccagtgt tcgacgttga tcaacagggt cgcccggtga tgcgctatat
     1141 cgaccagttc gtccagccaa aagattttga agaaggcgtg tggttgagcg agctttccga
     1201 tgccattgaa accagcaaag gcattctttc tgtacccgtt cccgttggca aattcctgtt
     1261 gataaacaac ctgttctggc tgcacggtcg cgaccgcttt actccgcacc cggatctgcg
     1321 ccgtgaactg atgcgtcagc gtggctattt cgcttacgcc actcaccact accagacgca
     1381 tcagtaagcg caaaggaatt gagcggatgt atgattttgt gattattggc ggcggcatca
     1441 tcggcatgtc gaccgccatg caactgattg atgtttatcc ggatgcccgc attgcgttgc
     1501 tggaaaaaga gtccggcccg gcctgtcacc agacgggcca caacagcggc gtgatccatg
     1561 ccggggttta ttacacgccc ggtagcctga aggcgcagtt ttgcctggcg ggaaaccgcg
     1621 ccaccaaagc cttttgcgat caaaacggca ttcgctatga caactgcggc aagatgctgg
     1681 tcgccacctc tgaactcgaa atggaacgga tgcgcgcgtt gtgggaacgc acggcggcaa
     1741 acggtatcga gcgcgagtgg ttaaacgcca tggaactgcg cgagcgcgaa ccgaatatca
     1801 ccgggcttgg cggcattttt gtgccatcca gcggcattgt cagctaccgc aacgtgacgg
     1861 cggcaatggc aaaaatcttc caggccagag gcggtgagat tatctataac gccgaagtca
     1921 gcggcctcag tgagcataaa aacggcgtgg tgatacgtac ccgccagggc ggcgaatatg
     1981 aagcatcgac gctgattagc tgttccgggc tgatggctga ccggctggtg aaaatgctcg
     2041 gcctcgaacc gggctttatt atctgcccgt tccgcggcga gtatttccgc cttgcgccgg
     2101 agcataacca gattgttaac catctgattt accctattcc cgacccggca atgccgtttt
     2161 tgggcgtgca tctcacccga atgatcgatg gcagcgtgac cgtcgggcca aacgcggtgc
     2221 tggcttttaa acgcgaaggc taccgcaagc gcgacttctc gtttagcgac acgctggaaa
     2281 ttttaggctc gtcggggatt cgccgggtgc tgcaaaacca tctacgctca ggactgggcg
     2341 agatgaaaaa ctcgctgtgc aaaagcggct atctgcggct ggtgcaaaag tattgtcccc
     2401 ggctttcgtt aagcgatctc cagccctggc ccgccggtgt gcgggcgcag gcggtatcgc
     2461 cggatggcaa gttgattgac gattttctgt ttgtcaccac cccgcgcacg atccacacct
     2521 gcaatgcgcc ctccccggca gcgacatcag caattcctat tggtgcgcat attgtcagca
     2581 aggtacaaac gctgttggca agccagagta accccggacg cacgctgcga gcggcacgta
     2641 gtgtggatgc cttacacgcc gcatttaatc aataaccttt gaaaacagga cgtaacaatg
     2701 aaacttaacg acagtaactt attccgccag caggcgttga ttaacgggga atggctggac
     2761 gccaacaatg gtgaagtcat cgacgtcacc aatccggcga acggcgacaa gctgggtagc
     2821 gcgccgaaaa tgggcgctga tgaaacccgc gccgctatcg acgccgccaa ccgcgccctg
     2881 cccgcctggc gtgcgctcac cgccaaagaa cgcgccaaca ttctgcgcaa ctggttcaat
     2941 ttgatgatgg agcatcagga cgatttagcg cgcctgatga ccctagaaca gggcaaaccg
     3001 ctggctgaag cgaaaggcga aattagctac gccgcctcct ttattgagtg gtttgctgaa
     3061 gaaggcaaac gcatttatgg cgacaccatt cccggtcatc aggccgataa gcgcctgatt
     3121 gttatcaagc agccgattgg cgttaccgca gctatcacgc cgtggaactt cccggcggcg
     3181 atgattaccc gcaaagccgg tccggcgctg gcggcaggct gcaccatggt gctgaaaccc
     3241 gccagtcaga cgccgttctc tgcgctggcg ctggcggagc tggcgattcg cgcgggcatt
     3301 ccggctgggg tatttaacgt ggtcaccggt tcggcgggcg cagtcggtaa cgaactgacc
     3361 agcaacccgc tggtgcgcaa actgtcgttt accggttcga ccgaaattgg ccgccagtta
     3421 atggaacagt gcgcgaaaga catcaaaaaa gtgtcgctgg agctgggcgg caacgcgccg
     3481 tttatcgtct ttgacgatgc cgatctcgat aaagccgtgg aaggtgcgct gtcctcgaaa
     3541 ttccgcaacg ccgggcaaac ctgcgtctgc gccaaccgtt tatacgtgca ggacggcgtg
     3601 tatgaccgct ttgccgaaaa attacagcag gcggtgagca aactgcacat cggcgacggg
     3661 ctggagaatg gcgtcaccat cgggccgctg atcgatgaaa aagcggtagc aaaagtggaa
     3721 gagcatattg ccgatgcgct ggagaaaggc gcgcgcgtgg tttgcggagg taaagcgcac
     3781 gaacgcggcg gcaacttctt ccagccaacc attctggtgg acgttccggc caacgccaaa
     3841 gtgtcgaaag aagagacgtt cggccccctc gccccgctgt tccgctttaa agatgaagcc
     3901 gatgtgattg cgcaagccaa tgacaccgag tttggccttg ccgcctattt ctacgcccgt
     3961 gatttaagcc gcgtcttccg cgtgggcgaa gcgctggagt acggcatcgt cggcatcaat
     4021 accggcatta tttccaatga agtggccccg ttcggcggca tcaaagcctc gggtctgggt
     4081 cgtgaaggtt cgaagtatgg catcgaagat tacttagaaa tcaaatatat gtgcatcggt
     4141 ctttaactgg agaacgcgaa tgagcagcaa taaagagtta atgcagcgcc gcagtcaggc
     4201 gattccccgt ggcgttgggc aaattcaccc cattttcgct gaccgcgcgg aaaactgccg
     4261 ggtgtgggac gttgaaggcc gtgagtatct tgatttcgcg ggcgggattg cggtgctcaa
     4321 taccgggcat ctgcatccga aagtggtggc tgcggtggaa gcgcagttga aaaaactgtc
     4381 gcacacctgc ttccaggtgc tggcctacga gccgtatctg gagctgtgcg agattatgaa
     4441 tcagaaggtg ccaggcaatt ttgccaagaa aacgctgctg gtcaccacag gttctgaagc
     4501 ggtggaaaac gcggtgaaaa tcgcccgcgc cgccaccaaa cgtagcggca ccatcgcttt
     4561 tagcggcgcg tatcacgggc gcacgcatta cacgctggcg ctgaccggca aggtgaatcc
     4621 gtactctgcg ggcatggggc tgatgccggg tcatgtttat cgcgcgcttt atccttgccc
     4681 gctgcacggc ataagcgagg atgacgctat cgccagcatc caccggatct tcaaaaatga
     4741 tgccgcgccg gaagatatcg ccgccatcgt gattgagccg gttcagggcg aaggcggttt
     4801 ctacgccgcg tcgccagcct ttatgcagcg tttacgcgct ctgtgtgacg agcacgggat
     4861 catgctgatt gccgatgaag tgcagagcgg cgcggggcgt accggcacgc tgtttgcgat
     4921 ggagcagatg ggcgttgcgc cggatcttac cacctttgcg aaatcgatcg cgggcggctt
     4981 cccgctggcg ggcgtcaccg ggcgcgcgga agtgatggat gccgtcgctc caggcggtct
     5041 gggcggcacc tatgcgggta acccgattgc ctgcgtggct gcgctggaag tgttgaaggt
     5101 gtttgagcag gaaaatctgc tgcaaaaagc caacgatctg gggcagaagt tgaaagacgg
     5161 attgctggcg atagccgaaa aacacccgga gatcggcgac gtacgcgggc tgggggcgat
     5221 gatcgccatt gagctgtttg aagacggcga tcacaacaag ccggacgcca aactcaccgc
     5281 cgagatcgtg gctcgcgccc gcgataaagg cctgattctt ctctcctgcg gcccgtatta
     5341 caacgtgctg cgcatccttg taccgctcac cattgaagac gctcagatcc gtcagggtct
     5401 ggagatcatc agccagtgtt ttgatgaggc gaagcagtag cgccgctcct atgccggagg
     5461 cgacgctgcg cgtcttgtcc ggcctacggg gatccaggtc ggataaggcg tttacgccgc
     5521 atccgacaat ctgtacgtga acaggaagaa atctatgttg gccgggtaag gcggagccgc
     5581 tctccggcaa aaagaatcaa taacaattat acgcgtgacc cgacgcggga aatgtcgggg
     5641 cgctctccca agtgacacac tttcgagagg attcaggatg gggcaatcat cgcaaccaca
     5701 tgagttaggc ggcgggctga agtcacgcca cgtcaccatg ttgtctattg ccggtgttat
     5761 cggcgcaagt ctgtttgttg gttccagcgt cgccatcgcc gaagcgggtc cggcggtatt
     5821 actggcctat ctgttcgccg ggctactggt ggttatgatt atgcggatgt tggcggaaat
     5881 ggcggttgcc acgcccgata ccggttcgtt ttccacctat gccgataaag ctattggtcg
     5941 ttgggcgggt tataccatcg gctggttgta ctggtggttt tgggtactgg ttatcccgct
     6001 ggaagccaac atcgccgcca tgatcctgca ctcatgggtt ccaggcattc ccatctggtt
     6061 attttccctc gtcattaccc tcgccttaac tggcagtaac ttattaagcg ttaaaaacta
     6121 cggcgaattt gagttctggc tggcgctgtg caaagtcatc gctatcctgg cctttatttt
     6181 ccttggtgca gtcgcaatta gcggttttta cccttatgcc gaagtgagcg ggatctcaag
     6241 attgtgggat agcggcggct ttatgcccaa cggtttcggt gcggtattaa gcgcgatgtt
     6301 gatcaccatg ttctcgttta tgggcgcaga aattgtcacc attgccgccg cggaatccga
     6361 cacgccggaa aaacatattg tccgcgccac caactcggtt atctggcgta tttctatctt
     6421 ctatttatgt tctatttttg tcgtcgtggc attaattccg tggaatatgc ccggactaaa
     6481 agccgtcggt tcttatcgct cggtactgga attgctcaat attccccatg cgaaattgat
     6541 catggactgc gtgatattac tttccgtaac cagttgcctg aactcggcgc tgtataccgc
     6601 ttccaggatg ctctactcct taagccgtcg cggagatgcg cctgcggtaa tgggtaaaat
     6661 caaccgcagt aaaaccccgt acgtggcggt gttactctcc accggcgcgg cattcttaac
     6721 ggtggtggtg aactattacg cgcctgcgaa ggtatttaaa tttctgatcg acagctccgg
     6781 cgctatcgcc ctgctggttt atttagtcat cgccgtttca cagttgcgga tgcgcaaaat
     6841 tctgcgagca gaaggaagcg aaattcgctt gcgcatgtgg ctttatccgt ggctcacctg
     6901 gctggtcatc ggctttatta cctttgtgtt ggtagtgatg ctattccgtc cggcgcaaca
     6961 gttagaagtg atctccaccg gcttattagc gatagggatt atctgtaccg tgccaattat
     7021 ggcgcgctgg aaaaagctgg tattgtggca aaaaacaccc gttcataata cgcgctgatc
     7081 atgatcagga gtcacaccat gaccattacg tctctggatg gctatcgctg gctgaagaac
     7141 gatattattc gcggtaattt tcaaccggat gaaaaattac gaatgagttt gctgacatcg
     7201 cgttatgcac ttggcgttgg gccgttacgg gaagctcttt cgcaactggt ggcggaacgg
     7261 ctggtcacgg tggtgaatca aaaagggtat cgggtggcgt ctatgtcaga gcaggagctg
     7321 ctcgatattt tcgacgcccg cgccaatatg gaagcgatgt tagtgagtct ggcgattgcc
     7381 cgcggtggcg atgagtggga ggcagacgtt ctcgcaaaag cgcatctgct gagtaagctt
     7441 gaggcctgtg acgccagcga gaaaatgctt gatgagtggg atctgcgtca tcaggcgttt
     7501 catacggcaa ttgtggcggg ctgtggttct cactatttgc tgcaaatgcg tgaacggttg
     7561 tttgatctgg cggcgcgtta tcgatttatc tggctgcggc gaacggtgct ttcggtggaa
     7621 atgctggagg ataaacacga tcagcaccag accctgactg cggcggtact ggcgcgagat
     7681 accgcgcgcg ccagtgagtt aatgcgccag catttactga cgccaattcc cattatccag
     7741 caggcgatgg ctggcaatta actactcttc cggaatacgc aacacttgcc ccggataaat
     7801 tttatccggg ctttttagca tcggtttatt cgcttcgaag attttattgt acagattagc
     7861 gttaccgtag acctgtttgg aaatggcact cagagtgtcg ccagacttaa cggtataaaa
     7921 ctggctggca gtggctggtg tcgccgtttt cacctgatca tcgacactgg caataccgga
     7981 aatattcccc accgcaacaa ggattttctc cttcgcctcc tgactcaggc cgtcaccagt
     8041 gaccgtcgct ttgccgtcgg caatttgaat attcacttta tcggcatccg gtataccggt
     8101 tttgttcaga tgctcctgca ccttcttcgc ctgatcgtct ttatcgtgct gacctgtaac
     8161 cgcgtcccag agtttttctc cggcatcttt cacaaaattg aacagaccca tagctacctc
     8221 attcgttaac ggaaacactc agaaagtgta gcagaggaac gcggcgcgtt aacgccgcgg
     8281 ggatgagcac aggtttaatc gcgggtttgc acccagaacg cgtgaatcaa accggggata
     8341 tacccaagca gtgtcaacag aatattaata atgaacgccc aaccgaaccc tttaccgagc
     8401 agcacgccga gcggcggcag aatgatggtg atgacgattc tccagaaacc catatgtact
     8461 ccctataaga aaattactca ttgtttaaaa agagatttta tctcttaagc gtagtaattt
     8521 tagcggagcc tgccagtttc agcttacgct tttactctca tttacgctgc tgaggctggt
     8581 atatgttgca tttttagttt agagtttact taatttagaa aatacttaaa taaatatgac
     8641 tgaactcgcg caattacagg ccagtgccga acaggcagcg gccttattga aagcaatgag
     8701 ccaccctaaa cgattgctga ttctgtgcat gcttagcggt tcccccggca ccagcgcggg
     8761 agagctgaca cgcattaccg gactgagtgc ctctgcgaca tcacagcatc tcgctcgtat
     8821 gcgggacgaa gggcttatcg acagccaacg ggatgcccaa cgcattctat attccattaa
     8881 aaatgaggcg gtaaatgcca ttatcgccac cctgaaaaat gtctattgtc cgtaaggagt
     8941 cactatggct ttgacaacca tttcgccgca tgatgcacaa gaattaatcg cacgcggcgc
     9001 aaagttaatc gatattcgtg atgctgatga atatcttcgt gaacatattc ctgaagcaga
     9061 tctggctcca ttatccgtgc tggaacagtc aggtcttccg gctaaattac gtcacgagca
     9121 aattatattc cactgccagg caggtaaacg caccagtaat aatgccgata aattagcagc
     9181 gattgccgcc cccgcagaaa tatttttact cgaagatggg attgatggct ggaaaagagc
     9241 tggattgcca gtagcggtaa ataaatctca accctttccg ttaatgcgcc aggtgcagat
     9301 cgctgcgggt ggtttaatat taatcggcgt tgtactgggt tataccgtaa atagcggttt
     9361 cttcttatta agtggctttg ttggtgccgg gttactgttt gcaggaatca gcggtttttg
     9421 tggaatggca aggttgttag ataagatgcc gtggaaccaa cgagcttgag aagcgacgcc
     9481 ggacgcgccc tagcagcgac atccggcctc agtaattaga tcaggaaatc gtcgagagat
     9541 ttaccttctg ccagcgcctg agcaattggc ttcggtgtac ggccctgacc ggtccaggtt
     9601 ttagtttcac cgttaacatc ggtgaattta tatttcgccg gacgcggctg gcgtttttta
     9661 ccagcgcgtg gtgcagcagc agagctatta cctaataact cttccgggtt aattccgtca
     9721 gccttcatca gctccagcca ggtgctaatt ttttcctgac gctcagccag ttcacgctgc
     9781 tgctgttctt cttcttcacg tctttcttta gtgacaaccc tgaatttttc gagcatttct
     9841 tcaagaacgt caatggagaa ttcgcgagcc atcgcacgga gagtgcgaat gttatttaaa
     9901 ctttgtaaca ttacggacat aataaagaaa accttttaac gccaaaacaa aaaagtattt
     9961 cgtcgcgtag tgtaagcgat cacttatt
//
LOCUS       DBANUU010000152         9921 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000152 DBANUU010000000
VERSION     DBANUU010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.154"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            6..81
                     /locus_tag="ACIE68_003930"
     tRNA            6..81
                     /locus_tag="ACIE68_003930"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACIE68_003931"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACIE68_003931"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(468..1226)
                     /gene="yhdZ"
                     /locus_tag="ACIE68_003932"
     CDS             complement(468..1226)
                     /gene="yhdZ"
                     /locus_tag="ACIE68_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFX5910175.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(1234..2337)
                     /gene="yhdY"
                     /locus_tag="ACIE68_003933"
     CDS             complement(1234..2337)
                     /gene="yhdY"
                     /locus_tag="ACIE68_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFX5910176.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2347..3528)
                     /gene="yhdX"
                     /locus_tag="ACIE68_003934"
     CDS             complement(2347..3528)
                     /gene="yhdX"
                     /locus_tag="ACIE68_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFX5910177.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3596..4621)
                     /locus_tag="ACIE68_003935"
     CDS             complement(3596..4621)
                     /locus_tag="ACIE68_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFX5910178.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5052..5273)
                     /locus_tag="ACIE68_003936"
     CDS             complement(5052..5273)
                     /locus_tag="ACIE68_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910179.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5526..8630)
                     /gene="acrF"
                     /locus_tag="ACIE68_003937"
     CDS             complement(5526..8630)
                     /gene="acrF"
                     /locus_tag="ACIE68_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFX5910180.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMEFGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8642..9799)
                     /gene="acrE"
                     /locus_tag="ACIE68_003938"
     CDS             complement(8642..9799)
                     /gene="acrE"
                     /locus_tag="ACIE68_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFX5910181.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2629 a         2578 c         2388 g         2326 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt gggggctccc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggctcagt cggaagactg ggccttttgt tttatttgat gtttgtcggt
      301 gaacactctc ccaaccagga caaatccgcc gggaacggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcaaaa
      421 aggccatccg aaaggatggc ctttttgctt ttcgaactaa cattcaatta atggattacc
      481 tgcgataaaa atgccctcgt acgctctgat ttaggatgcg taaaaaattc atcaggtgca
      541 gcttgctcca ctatttcccc acgatccata aaaatcaccc ggtcagcgac ggttcgcgca
      601 aaccccatct catgcgttac acacaacatt gtcatacccg attgcgccag cccaatcatc
      661 gtatccagca cctctttcac catctccgga tcgagcgccg acgttggctc atcaaacaac
      721 ataattttcg gcttcataca cagcgaacgc gcaatggcaa cgcgttgctg ctgaccacca
      781 gaaatttgtc cgggaaactt atgcgcatgt tcggcaattc tcacccgctc aaggtaatgc
      841 atcgccagag cttcagcctc tttctttggc atcttgcgta cccaaatcgg tgccagggta
      901 cagttctgta gaacggtcag atgagggaag agattgaaat gctgaaagac cattcccact
      961 tcctgccgga cgcgctcaat attgcggata tcttcattaa gttcgatgcc atctaccacg
     1021 atccgtccct gttgatgttc ttccagatga ttaatacaac gaatggttgt cgatttaccg
     1081 gaacctgaag ggccacacag aacgatccgt tctcccggtt gcacggttaa atttatattt
     1141 ttcaaaacat ggaattgtcc ataccattta ttgacgtttt ccagcgtaat catcgcgtta
     1201 gcaggttgca gtaaaatttg gctcatagtg tcctcaatgc ggtgtacgcc cggtgttaaa
     1261 acgtttttcc agatgctggc tatagcgcga catgctgaaa cagaagatcc agtagatcag
     1321 cgcggcgaaa acataccctt ccgtcgacat acccaaccag gtgggatcaa cggttgcctg
     1381 ctgaacgcta ctgaaaagat cgaacaaccc gataatgatc accaggctgg tatctttgaa
     1441 gagtgcgatg atggtattta ccagcccagg aattaccagc ttcaacgcct gtggcagaat
     1501 aaccagcccc tgagttttcc agtaacccaa cgccagcgac tctgccgctt catactgccc
     1561 tttaggcagt gcctgtaatc cgcctcgcac gacttccgca acatatgctg actgaaacag
     1621 gatcacgcca accagcgcgc ggatcagttt gtcgatactg gtgccttctg ccataaacaa
     1681 cggcagcatg accgaagaca taaacaatac ggtaatcagc ggtacgccgc gccagaactc
     1741 gataaaaatg accgataaga tacgcacaat cggcatatgg gagcggcgac ctaacgccag
     1801 taagatcccc cacggtagcg cccccgcaat cccaacggat gcaataatta aagttagcgt
     1861 cagcccgccc cattgccggg tttcaacccg ctccagacca aaaaaaccgc catacatcag
     1921 ccaccagaca atcagtgggt aaatcaccgc ccagaccgca atatagcgac cgcgatgcgg
     1981 gagtattttc cagaacattg gtgcgattga cacaaggcca atcagtaatg ccaggttaat
     2041 tcgccagcgt tggtcgtgtg ggtaaagtcc atacataaac tgaccaaatc gttcgtggat
     2101 gaagacccaa caggcaccgg cttttgtgca gtcggcacgc gttgagccaa cccaattagc
     2161 ctgcaaaaat gcccagttca gcaacggtgg gatcagttcc cacatcaacc atatgcagcc
     2221 aatagtcagc aggctattgc tccagctgga gaacagattt tttcgcaccc acaccatcgc
     2281 gcggcttgag ttatggctcg ctgggcgcga gggctgagac agcaatactt ttgtcatcat
     2341 gactccttag cgttcaacga tcgcgatgcg gcggttatag atattcatca gcagcgagat
     2401 agtcaggctg ataatcagat agaccgacat ggtcatggct atcgtctcga tggcttgccc
     2461 cgtctggttc agcacggtgc cagcaaacag cgaaaccata tcgggatagc caatagcggc
     2521 ggcaagagag gagtttttga cgatgttgag atactggctg gttaacggcg gaataatcac
     2581 tcgcagtgcc tggggaataa tgacctggcg tagcgtaacc gggttgggta atcccagtga
     2641 ccgagccgct tcatgttgac cataaggcac tgcctggatc ccagcgcgga taatctcggc
     2701 gataaatgca gatgtataaa ccgaaagtgc cagcgttaag gctgccagtt caggaattaa
     2761 aaccatcccg ccgcggaagt taaagcctcg tagggctggg acatcccagt gtagtgctgc
     2821 gccaaaaagc cattgcgcca gtaaaggcaa accaatgatc aacactgcgg cgatcggcca
     2881 ggtgcggcgc agttgtccgg tctttatctg gtatgtttta ttaaagcgga atagcccaac
     2941 agaaaggact atagccataa caacagccag gataaacgca ataaatccgt ctcccaactg
     3001 cggtgacgga atataaagcc cacgattgct caaaaaggcc agatcaaacg cgctcacagc
     3061 ctggcgtggt ccgggcaaat tgcgcaacac ggcaaagtac cagaagaaga tttgcagcag
     3121 cgggggaata ttacggaaga tctcaatata aattgtggaa agctttcgta gcagccagtt
     3181 atccgaaagt ctcgccagac cgataaagaa gcccaaaaca gaagcgaaca caatacacaa
     3241 tgcagaaacc agtagcgtat tgagtaagcc gacaataaaa acgcgtccgt aggtgtcgcc
     3301 ctgctggtaa tcgatcaaat gctggacaat accgaagcca gcgccgcgat ccagaaaggc
     3361 aaaacctgaa gtaatgccac gattattgag attcgttaca gtgttgtgaa ataaccaacc
     3421 aacaatgccg acaacagcaa caacggcaag gatctggaat aaccaggcac gaaccgtagg
     3481 gttggcaaag gagagtgagc ctttaacggt tgagcggcga tgagacatag aaacctcgga
     3541 aagcataacg ctgaagagag cgccgccgtc gcgacgctcg ttacctcaca ccgacttaac
     3601 gcacgggcgg tgcgtactga ataccgccgt tattccagag attattttgc ccacgtttaa
     3661 ttttcagcgg gctttctgaa cctacgttac gctcaaaaat ttccgagtag ttacccacct
     3721 gtttgatgat gttataggcc catttattat ccaacttcag atccttgccg taatcgcctt
     3781 ctttacccag cagatgtgcc atatcaggcg ttgctggatt agccgctttt tcatcaacgt
     3841 tctgggaatt gatgcccatc tcttcagcat tcagcatggc gaaaagcgtc cagcgtacaa
     3901 tcgagaacca ttcatcatcg ccacgacgaa ctaccggccc aagcggttct ttagagataa
     3961 cctccggtaa gacaatccat tcagctgggt tgcttaattt gatgcgcagg gcatacagtt
     4021 gcgactgatc cgaggccagc gtatcgcagc gaccagattc cagtgccttc gctgattcgt
     4081 cagagcgatc gaaagtcact ggtgtgtact tcatattgtt tgccttgaaa tagtcggcaa
     4141 cgttgagttc ggtatcagta cccgcctgaa tacagacggt agcgccatcc agttctttcg
     4201 cgctttttag ccccgcttta tcgtgcgtca ggaagccaat gccgtcgtaa taagtgacgc
     4261 cagtaaatgc cattcccatc ccggcatcgc gagatgaagt ccaggtcgta ttacgggaga
     4321 gcaaatccac ttcccctgac tgtaaagccg tgaagcgttc ttttgctgtg agtggggtat
     4381 atttcacttt cgtgtcgtca ccaaatacag cagcggcaac accacgacaa acatcaacat
     4441 caatacctga aaacttaccg tctgcatcgg cataagagaa cccaggtaat ccatcactga
     4501 tcccgcattg cacaaaacct tttttctgaa cggcatcaag cgtcgcgcca gcatgcgcct
     4561 gatttgcaac ggcaagcagc acgctggcgg cagccagtgt ggctatcatc atctttttca
     4621 taatgcttcc tgtgaggcga aaattatcgt tataggacgt ttgggaaacg tcttttacct
     4681 gtctgtggcc atcctcttat aagcaaaagt agtgccagct tttgcgcccg atttcgcgtc
     4741 gcaataagct gagtgtagac aggaaataat aaaaacagcg ccccgcaatg gtgcgaaaat
     4801 gaaacatgcg ccacattttg gcgcaaaata tttacgcgtg ttttactaag cagaataatg
     4861 attgttagcg tgagtatatt tctggtggga agtgtgaaag ttaaattgat gcgttatttc
     4921 gaaagcagcg aaataacgcg atataacatg ctaaatcagc agagaattag ccagcagaat
     4981 atagcaatat tttatcttat gtaaagatac tgtatcgtta aacagagcgc gaaactccgc
     5041 gccctgttca gttatttcga cagagcgatg atccccatcg tcaggccagc aacagccgct
     5101 gccccgccag caatagcccc agccgtttcc cagttatcct gagtcgtgcc tttcatacag
     5161 gtattggtgt gaaccagacg accctgatcg tcataaaccg gaacgcaggg cgaatcgtga
     5221 gcacagcccg ccagcaatgc ggtgagcaat gccacaggaa ttaatctttt catggtgtta
     5281 cctcagtaat aaacgctaac gcttaataag ttaactctct tattaacgag ctgaatgcca
     5341 ttttatcacc ggaggttata aatcttgcgg aatgaataat gacaaattat gtgaattgta
     5401 aaaatagtga agggagcggg aactatagaa agcttttaaa gagtgagcag aagaatatct
     5461 tattaatttt tatgggtttc actggaaata ataaaggcac ccgaaagcgc ctttatgttt
     5521 ctgatttatc ctttaaagca acggcggatc accacaaaga acaccggtac gaagaagatt
     5581 gccagcaacg ttgcagagac cattcctccc attaccccga tacccacagc gttctgcgcg
     5641 ccactgccgg caccgttact gatagctagc ggtaatacgc cgagaataaa ggcgagagag
     5701 gtcatcagga taggacgcag acgcatacgt actgccatta gtgtcgcttc aacaacacct
     5761 ttaccctctt tctccatgag atctttagcg aactcaacaa tcaaaatagc gtttttggcc
     5821 gacaagccaa ttgtcgttag caagcccacc ataaagtaga cgtcattttt ttgattaaag
     5881 agtgtcgccg ccagcagcac gccgacaatc cctaacggca cgaccaacat aaccgagaca
     5941 ggaattgacc agctttcata gagtgcagca aggcacagga aaacaaccac aaaggaaatt
     6001 gctaccagag cgggagcctg gtttcccgat aagcgttcct gatacgacat acccgtccag
     6061 tcataaccaa tgccagcagg taattttgac gcaaggtttt ccatcaacgc catggcatcg
     6121 ccggaactgg ttcctggcgc ggcttccccc tgaatctcca ttgacggcag accgttgtag
     6181 cgttccagtc gcggagagcc atacacccaa tgtgaagtgg taaaggccga gaatggcacc
     6241 atttcgccgt tggcgctgcg gacataaagt ttatcgacat cttctggcag catacggaat
     6301 ttggcatccg cctgaacata cacctttttc acgcgaccac ggtcgatgaa gtcgttaacg
     6361 taagtcccac ccaacgccgt tgaaatggtc tgattgatgt cagaaagtga aacacctaat
     6421 gcctgcgcct tttcctggtc aacttccagt ttaaactgcg cggtgtcttc caggccatta
     6481 ggacgcacgc tgactaagct ggcaggatgt tgcgccgcca taccaagcaa ctggttacgg
     6541 gcctgggtta gggcatcgtg acccagccca gcctgatcaa ttaactcaaa gtcgaaaccc
     6601 gttgccgtgc ccagttcaac aatggctggc atattgaatg gaatgacaaa accgtcgcgg
     6661 atcttgccaa attccatttt ggcacgatgg attaccgctt ccgcactgtt ttcatcacca
     6721 ttacgctctt cccacggttt cagactgacg aaggccatac cggcgttttg tgcctggccg
     6781 ctgaagctaa agccgttaac cgtaaagaca ctttcaacgt tcgctttctc gttcttcaga
     6841 tagtaatccg tcacttgatc caacactttt tgcgtccgct cttgcgtcgc gccagcgggt
     6901 aactgaatca tggtcagaaa gacaccctga tcctcttcag gtaagaagga agacggaaga
     6961 cgtaaaaaca acaccaccat tcctgcaaca atcagcgcat agatcagtaa atatcgtcct
     7021 gtggaaccga ggattttgcc gacgctgttg gtgtagtggt taacgctatg atcgaaggta
     7081 gtattaaacc agccgaagaa accgccctta ttttcgtgat gctcagcaga gacgggttta
     7141 agcagcgttg cacataacgc aggggtaaga atcaatgcca ccagaacaga aagcgccatt
     7201 gccgaaacga tggtgataga gaactggcga taaattgccc cagtagaacc gccgaagaat
     7261 gccatcggaa taaataccgc tgacagcacc atcgcgatac ccaccagtgc gccctggatt
     7321 tgcgacattg atttttccgt cgcttctttt ggcgggagct tatcctccat catcacgcgc
     7381 tcgacgttct ccaccaccac tatcgcatca tcgacgagca gcccgatggc aagcaccatc
     7441 ccgaacatcg ttagtgtgtt gatggagtaa ccaaaagcgg cgaggatggc aaacgtccct
     7501 aacaacacca cgggtaccgc aatggtgggg atcagcgttg ctcgcatatt ctgcaagaac
     7561 agatacatca ccaggaacac cagcataatg gcttcgaaca gcgtttttac cacttcgtga
     7621 atagaaagct ggacgaatgg cgtggtgtca taagggtaga gaaccttcat tccctgcggg
     7681 aagaatggct gtaattccgc cagttttgcc ttaatggctt tcgcggtatc gagagcattc
     7741 gcgccggttg ccagcttaat ccccaggccc gccgccggtt ttccgttgat acgagcgata
     7801 acgttatagt tttcaccgcc aagttcaacc cgtgcgacat ctttcaggcg taccaccgag
     7861 ccgtcactgt ttacgcgcag ggtcactttg ccgaattctt ccggattttt taaccgcgtt
     7921 tgagcaataa tcgaggcatt caactgttgc cctggtaacg ctggcgttcc gcccaactgt
     7981 ccggcagcga tctgatcgtt ctgtaccttc aactggttaa tcacatcaac cggtgtcagt
     8041 ttatatttgt ttagcagatc ggcgtccagc cagatacgca tcgcatactg tgcgccgaaa
     8101 agctgtacgt caccgacgcc attcagacgg ctaagcgtat ctttaacgtt agaggccaca
     8161 tagtccgaga tatcgtcctg tgtggtgtct gggttatcag agacaaagcc cgccaccatc
     8221 aaatagctgc tactggactt ttcaacactg atcccctgct gctgaacctc ctgcggcagc
     8281 aacggcgtgg cgagctggag tttgttctgc acctgcactt gcgcgatatc aggatcggtc
     8341 ccggactgga aggtaagggt aattgtcacg ctaccggcgg aatcgctggt ggaggacata
     8401 tacatcaggt tatcgatacc attcatattc tgttcgataa cctgcgtcac cgtatcctgc
     8461 acggtctgcg catcagcacc tggatagttt gccgaaacag aaaccgcagg cggtgcaatg
     8521 gttggatact gagcgacggg caattgtagg atcgccagtg cgcccgccat catcagaata
     8581 atggccagca cccatgcaaa tatcggtcgt cgaataaaaa agtttgccat gtcagattac
     8641 cttacttcga tgcagtatct gcgggggtat cggtagtggc tttaacctgc tctcccggac
     8701 gcgctttttg caggccgctg acaatgactt gatcgccaga tttcagacct tcgctaatca
     8761 accatttatc gccaatcgcc tgactggcaa cgacagggcg cgcttccacc tgacttttat
     8821 cgttaacaat cagcacggtt gcatcaccac gcggtgtgcg gctaacgcct tgttgcggga
     8881 taagaatggc gtcaggttgg acgccttcat caatccgtgc acgcacaaac atacccggca
     8941 aaagcgtatg ttgcgggtta gggaagacag cacgtagggt tatggagccg gtgctttcat
     9001 caacggtcac atcagagaat tgcagcgtac ctttcagggg ataggtttga ccgttttcca
     9061 tgaccaactc tacgttgctg gtggcgtttt ccttatgcaa atttccttgc tctacggatt
     9121 gcttcagcct cataaaatcg ttgctggatt gggtcacatc aacgtagata ggatcgagct
     9181 gctggacagt cgccagttca gtcgtttgcc cattagtgac aagagcgcct tcggtcacgg
     9241 tcgatttgcc gatacgtccg ctaattggcg cagtcacttt ggtataagcg agattgatgc
     9301 gagcgctttc gactgtggct tttgcggcaa tcacggcggc atcggcctgg cgagcatcag
     9361 caatggcctg gtcgtactcc tgctggctga tgtatttcgt acccacgagc ggaacgtaac
     9421 gttttaccgt caaatgcgcg atggcggcgg cggcttcact tttcgccagt tcgcctttcg
     9481 cgctgtcata atttgcctga taggtcgcgg gatcgatctg gtacagggac tggcctgctt
     9541 gtacatcgct gccttcagtg aaattacgat tcagtacgat cccgctaacc tgtgggcgaa
     9601 cttcggctat acggtaagca ttggtgcggc ctggtaattc agtcttaact tctaacgggg
     9661 ccgttttcac aatatgaacg gtaacctgcg gttcaccgac gtgagctttc tcttctccct
     9721 tatcgttaca accggcgatt aaagccgcgg agatcagaat aaaggagggc aggaggaaaa
     9781 acctggcatg tttcgtcatt actattcctc aaaaaaccaa aagcgcgtta tttacccaga
     9841 aaggcaacgc gttaactcgc agaaagaaaa atacagtttg ctatcctaca aattatcatt
     9901 cgtcgatgta aggaatagtt a
//
LOCUS       DBANUU010000153         9798 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000153 DBANUU010000000
VERSION     DBANUU010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.155"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACIE68_003939"
     CDS             complement(<1..149)
                     /locus_tag="ACIE68_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910182.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSL"
     gene            227..1321
                     /gene="ddlA"
                     /locus_tag="ACIE68_003940"
     CDS             227..1321
                     /gene="ddlA"
                     /locus_tag="ACIE68_003940"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFX5910183.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1345..1557)
                     /gene="yaiZ"
                     /locus_tag="ACIE68_003941"
     CDS             complement(1345..1557)
                     /gene="yaiZ"
                     /locus_tag="ACIE68_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFX5910184.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1817..2125
                     /gene="yaiY"
                     /locus_tag="ACIE68_003942"
     CDS             1817..2125
                     /gene="yaiY"
                     /locus_tag="ACIE68_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFX5910185.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2184..3278)
                     /gene="yaiW"
                     /locus_tag="ACIE68_003943"
     CDS             complement(2184..3278)
                     /gene="yaiW"
                     /locus_tag="ACIE68_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFX5910186.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVGSVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPPFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3291..4511)
                     /gene="sbmA"
                     /locus_tag="ACIE68_003944"
     CDS             complement(3291..4511)
                     /gene="sbmA"
                     /locus_tag="ACIE68_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFX5910187.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4863..6020
                     /gene="ampH"
                     /locus_tag="ACIE68_003945"
     CDS             4863..6020
                     /gene="ampH"
                     /locus_tag="ACIE68_003945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFX5910188.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKQLVIPAS"
     gene            complement(6021..6644)
                     /gene="iprA"
                     /locus_tag="ACIE68_003946"
     CDS             complement(6021..6644)
                     /gene="iprA"
                     /locus_tag="ACIE68_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFX5910189.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6732..9683)
                     /locus_tag="ACIE68_003947"
     CDS             complement(6732..9683)
                     /locus_tag="ACIE68_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFX5910190.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAANYDTWGYHDNTTTDLV
                     WPYDTDNDGIADAGGMDPMGSDGNYVTYNGFVYYNNANGDFDTVFKGDTVNGTISTYY
                     LNHDYASGSVNQVDISNSVIHGSITSELPFGYYSITPEGYNGYTFNNGTTTDLDYNWY
                     DGDVFTLNVSNSTIDDDYEALYFTDTHLDGDKSKSTNETFYTALGVAVNLDVESNINI
                     TNNSRVAGIALSEGNTSNTTYTSEYHQWDNNINVSNSTVTSGSQTPLEESDGFFGKSA
                     EPSDYAGNGGQNDVALSFTDNAGSNYSMKNNVNFDHSTLLGDVEFTSHWNNDGVFFYS
                     TGHDSNGDGVLDTNGGWVDDAQNVDELNITLNNGSKWVGSANMSAEVIAPADMYDVAP
                     NSLTPGATIEANDWGRIIDNKVFQSGVFNVALNNSSEWNTVNSSVIDTLAVNNGSQVN
                     VTDSSLVSDTIGLTNGSSLNIGANGVVATDHLTVDSYSTVNLTESTGWNNYSNLYTNT
                     ITVTNGGVLDVNVDQFDTEAFRTDKLELTSGNIADHNGNVISGVFDINSSDYVLNADL
                     VNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDVDNGTELDNSSVDNVVAATGNYKVR
                     IDNATGAGTIADYKDKELIRVNDANTNATFSAANKADLGAYTYQAEQRGNTVVLQQME
                     LTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGT
                     INYDQDVNGIMVGVDTKVDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSA
                     HFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYV
                     TPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKL
                     AYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQD
                     WSANVGVKYTW"
BASE COUNT         2534 a         2422 c         2314 g         2528 t
ORIGIN      
        1 ttaagagaaa aaaactcacc gcattgccat ttaagccagg aataaattag cttcccgttc
       61 atcatttttg tgatcttgcg cacatctttg agataacgca ctttcttcac cagtacgttg
      121 cacgctttgt cagcagaaga ttgcaacaat ctttcagaag gccgcgaatc ccccgttttg
      181 tcgtggtaat gtatgcactt tgtgttagac gggataggtt tttaagatgg aaaaactgcg
      241 ggtaggaatc gtttttggtg gtaaatcagc ggaacatgaa gtgtctctgc aatcggcaaa
      301 aaacattgtc gatgccattg ataaaagtcg cttcgacgtt gtgctgctgg gcattgataa
      361 acaagggcaa tggcacgtca gcgatgccag caattatctg ctaaatgcag acgatcctgc
      421 ccatattgcg ttgcgccctt cggcgaccag ccttgcgcag gtgccaggta aacatgagca
      481 tcagcttatc gacgcgcaaa acggtcagcc gttgccgacg gtggatgtca ttttcccgat
      541 tgtccacggt acgctgggcg aagatggttc cttgcaggga atgctgcggg tcgccaattt
      601 accgtttgta ggttctgatg ttctggcttc agcagcctgt atggataaag atgtcaccaa
      661 acgtctgcta cgcgatgccg ggctgaacat tgcgccattt attaccctga cgcgcgctaa
      721 tcgtcacaac atcagttttg ccgaagtgga gtctaaactg gggttaccac tgtttgtaaa
      781 accggctaat cagggctctt ctgttggtgt cagcaaagta accagtgaag aacagtacgc
      841 aattgccgtc gatctggcgt tcgagttcga tcataaagtg atcgttgagc aagggatcaa
      901 aggtcgtgag atcgaatgcg cagttctggg caacgacaat ccgcaagcca gcacctgtgg
      961 cgagatcgta ctcaccagcg atttctatgc ctacgacacc aagtacattg acgaagatgg
     1021 cgcgaaagtg gtagttccgg cagccattgc accagagatc aacgataaga tccgggcgat
     1081 tgccgttcag gcttatcaga cgttgggatg cgcaggcatg gcgcgtgtag acgtgttttt
     1141 aaccccagag aacgaagtgg tgatcaacga gatcaacaca ctgcctggct tcaccaacat
     1201 cagtatgtat ccgaagctgt ggcaagccag cggtctgggt tacaccgatc tgatcacacg
     1261 tttgattgag ctggcgctgg agcgtcacgc tgcggataac gcattgaaaa ccacaatgta
     1321 atattttaac gcccgataat attcttattg ggcgttttca gtttcttcat cgcgacgacg
     1381 aataatcaac cccgccagcc agaaactaat cacccacgtc accagaccga cggcataggt
     1441 ctgccaacct tttgcttcaa atcccagtaa ccccaccacg ccattaagaa tgaatataag
     1501 gccaatggcg aacgcatagt agtgccagtc acggcggatt tttacaggca gattcatggc
     1561 agctccgtta acgaaaaaca acatccttgc atattctgta acaaccgtct gtggtgtaga
     1621 caacactttc gcaattgtta ataaatttaa cttaaagttg ctgttttgtg actcgcaacg
     1681 aagattcacg cttcgggaat attcccaaca tcaatttacc atgattctga tattgacaat
     1741 ctaaatgacc tgtcagactt gccataagcc gctggtatca caccagaaga gaagcagatg
     1801 acttccggag gtttttatgg ctgatttcac cctgtcaaaa tcgctgttta gcggaaaata
     1861 tcgcaatgcc tcttcaacgc ctggcaacat tgcctatgcg ttgtttgtgc tgttttgctt
     1921 ttgggctggg gcgcaattgc tgaacctgtt agtgcatgcg cccggcgtct atgagcgttt
     1981 aatgcaagtc caggaaacag gtcgcccacg ggtggaaatt ggtttaggtg tcggcaccat
     2041 tttcgggctg atcccgtttt tagtaggctg cctcattttt gcagtggtgg cgctatggct
     2101 gcactggcga catcgccgcc agtaagtttt acgttgtcag atgcggcctg aaacgcctta
     2161 tctggcatga cattcctgtt tgatcactgt ttcatacaac gcgcccgccg ttcgtctacg
     2221 cgcttcgcaa accaggccgt agtcaggttg cgggtgattt tcgggctttc cagttgaatg
     2281 ccaggcaaca tttcgcgggg tagtgattta ccggttttcg cttcggcaag ttgataaact
     2341 ttcttgtaca gcgccgtctc ctcaaaagag aagctgtcac ctttctctag ctgacgacga
     2401 atttcgctgt cgctcatccc cagttttgcc gccagtttgc gcgttgccag ttctgttttc
     2461 ccgggttctt tactgtcgta acgaattaaa tcaccgtcca gtgccagctt cacgccgctg
     2521 gccttactga ccgcgttctg aaacgcggca ttacggctgg cgtaccagcc agcgttaaaa
     2581 tcagcaaaac ggtatatcgg tgcactataa ctggcgggat aattcagtaa atggtaagta
     2641 ccaaaccaca acccgccgcg gcgactaaag acttcctgac gaactgtacc gtccattttc
     2701 cacggatacc ctttggtatg ctgttcggca aaagcaatgc tgacctgcat cggcccaccg
     2761 gtgcgcaccg gattaagcga accaaacagc gtctgcccca ttggcaccat gctgatcaga
     2821 tcgtcaaaaa tcgcgctcaa ttgcttttct gtacgcaccg aatctaaccg ttcgctataa
     2881 cttttgccgt ttggcgattt gatttttagc gcggtatgaa ccagaaaagg aggaatgtgc
     2941 atccgttcag cacgacggtc aatctcttgc caggcgatct tgcttaagcc cggaacggcc
     3001 ggatctgcct gataatttga ctcctgctgc gccaccgcca gcaccgagca gacgttttcc
     3061 agcgtcggtg ccagcccctg actttcaaag gtggtggcga gatctttcgc ccaggcatcg
     3121 cggtctttca cgctcgcagg catcttctgc cgcacgacgc tccctacatc tatcgctttt
     3181 tcgccctttt tcaacggttg cggagcctga ctactacacc ccgctaaaac cagaaccgcc
     3241 aacaatgaca gcgaagatag aggattgacg cgcgacatta cttctccttt ttagctcaag
     3301 gtatgggtta cttcctgaat tttgtcacca tccagctcat gttcaaagct gcgcagacgt
     3361 ttgtagatag acatcaactc aaccagtgtg gtccatgagt taatcaggta ctggaaagca
     3421 ccgcgaacct gaccaaaaac gttggtaatc tgcgtcatca ggccgagcgt aatcgtaccg
     3481 gcaacaattg acggaaacag caagaacaaa ccgaaaacgt tatcgacctg caaatagaga
     3541 atgcgggcga tgttgaaata catatagtga aaatagaggc gaaaatagtt tttccgtacg
     3601 gcgctaaaca gctcgcgtac cgtaggcggc gtcgcgcgcg tggcatcatc ttcaccataa
     3661 accagctctt tacggtaggc agcctctaca cgctggtttt taaactccag ccccggcagt
     3721 ttgatcccta ccactgccag caatccggtc cccatcagcg accagacgat agcggcaatc
     3781 accagaccat acggaatgtg cccgataatc ggcagctccg gcacatgcgc ggagagcgtt
     3841 accagtaccg gcaggaaggc gattaacgtc atgatggcgt tgataaaact gacgcccata
     3901 ttctccagcg ttgaagcaaa acgcatggtg tcttcctgca cacgctgtgc ggcaccttcg
     3961 atatgacgca gttgttgcca gttcgccatg taatattcgt tcatcgccgt acgccagcgg
     4021 aacacgtagt gactgacaaa gaagttattc agcacactga tcactaccgc gatcagcgca
     4081 atccccagaa agacgcccac ttcgcggtaa aattgttcga tggtgacttt atgcggcgaa
     4141 cttagcgcgg tttgaatcag atcatagaac ggcgcatacc aggcgttgac ggcaaccccg
     4201 acttccacca aaaaccaggt gacgaagata atcagcgcag taccgagtat tgaccagtat
     4261 tgccaacgat gcgggctgta gataaaccag aacaatgcaa aaagtcctac gcaaacaatg
     4321 tagtaagcgt aaaaaatcag gaaatccaac gaccagaaac gcgcggcgct aatcgggatc
     4381 tgcccggaag cgccggtgat acgcgccacc cagtcacccc caccggcttg ccagaagata
     4441 acggcaatca atgcccaaac aaaggccgag agaaaaaacg ttcccggctt tgggaaaaaa
     4501 gacttaaaca tatgcactcc tgctaacttc ttatcgtttt aacaactgct gtgtattaac
     4561 cgcatgaccg ctacaaagcg tcagccaaat gaaaccgccc attgtgaccc atagcaaagc
     4621 gcttagttcg ccagcctgac cataagaatt gtttcgacgg gtttcacaaa ccaaagattg
     4681 tttacataag cggattaaca cgcgcaggat cacaaatgca ggcattggtg agcaggaaaa
     4741 aaatcgtgtt tacgcgccgt tacgttttta acactattat tgcactaatt cgccctttgc
     4801 aatccatcaa tgagtagtat aaatacgctc agttaccttc attcaattta tggacaccac
     4861 cgttgaaacg tagtctgctt ttttctgccg tgctgtgtgc ggcgtcattg acctccgtcc
     4921 atgcggcgca gccgatcact gaaccggagt ttgcctctga tattgtcgat cgttatgccg
     4981 atcatatttt ttacggcagc ggtgccacgg ggatggcgct ggttgttatc gacggtaacc
     5041 agcgcgtctt tcgcagttat ggcgaaacgc gacctggtaa taacgttcgc ccgcagctgg
     5101 attcggtcgt gcgtattgct tccctcacca agctaatgac cagtgagatg ctggtgaaat
     5161 tgctcgacca ggggaccgtg aagttaaacg atccgctaag taaatatgct ccgccaggcg
     5221 cacgcgtgcc aacctacaat gggacgccca ttacgctggt caatctggca acccatacca
     5281 gcgccctgcc ccgtgaacag cccggtggcg cggcacatcg tcccgtgttt gtctggccaa
     5341 cgcgcgagca acgctggaaa tacctttcta cggcgaagct gaaagccgcg ccaggcagtc
     5401 aggcggcgta ctctaacctt gcgttcgacc tgctggcaga tgcactggcg aatgcgtccg
     5461 gcaagcctta tacccagttg tttgaagagc agattacccg tccattgggg atgaaagaca
     5521 ccacctacac cccctcaccg gatcagtgcc gccgtttaat ggttgctgag cgcggtgcca
     5581 gtccgtgcaa taacacgctg gcggcaattg gcagcggcgg cgtctattcc acgcctggcg
     5641 atatgatgcg ctggatgcag cagtatttgt catctgattt ttatcagcgc agtaaccagg
     5701 cagatcgcat gcagacgttg atttatcagc gcgcgcagtt taccaaagtg attggcatgg
     5761 atgtccccgg caaagcggat gcgcttggtt taggctgggt atatatggcc ccgaaagagg
     5821 gtcgtccggg gattattcag aagacaggcg gtggtggcgg tttcattacc tatatggcga
     5881 tgatcccgca gaagaatatt ggcgcgtttg tggtggtcac tcgttcgccg ttgacgcgct
     5941 ttaaaaatat gagcgatggc atcaatgatc tggtgaccga gctaagcggg aataaacagt
     6001 tggttatccc cgcatcctga ttaatactct gaaggcaaac ggttgatagc gaccagtttg
     6061 cctttattca tttcgatata gccgccttta cgcaaagcag cgagaacttc tgcgacgaca
     6121 gaacgcgata tgcgtgtacg ttgatggata taattcatca caccaatacg tgatcgcaat
     6181 tcttcattcc agtcaatcat tgataataat gttgcgcgga tttgttcgta ggaattatgc
     6241 ccaatgagct gcacgtcacg taattccaga attctatttt gccaggctaa ccagtaaaaa
     6301 gcgtctcgcc agagttgatt ttgttcaata agcgtaatgg tttgtttggc tggtagatga
     6361 tatcccgtac aatttccttc tgatattaat ttgtatggta tatcgttttt cattaaacca
     6421 tcagccagcc ccataatata aggagcctgg gtaataccga taagtacgtt ttcttctcta
     6481 cgcagagaga taactccctc taaaataacg aaagtatctt cgttattgac atcactggaa
     6541 aatataactt gcttttcatt attaaactcg aagcgcgtac cgtatctgga caaacattta
     6601 tcgagcttac caaattcctg aagaggttta actacagata acatttgcgc gtcctttgca
     6661 gtaatgcccg tcaaatcctt gacgggcatt atttagatta aattaccaga atttcttcgg
     6721 agtgaagaat attaccaggt atatttaaca cccacgttcg cggaccagtc ttgatctacg
     6781 tcaccaccac cgaggtagtt agcatcggta taggcgctaa agttcttggt gaagctgaac
     6841 tgagtaccca gaccaacacg taccgcagac ccttcagtac cgttatcgat ggaatcaccg
     6901 ttcacatcgt tatcgttgtt agagtcgtcg tagacgtaag ccagtttgaa gtacggagtc
     6961 agagcctgat cttcgctgta ggtgaaggta taacctgcat ctacacccag ttcataacgc
     7021 atgctgtcgt aagactgacc gtcaactttc atgtcgttgc tcagctggta gtcatcacca
     7081 gactggaaca gaccagaaac gctgccgtaa ggagtcacgt aaccagcatc acccagtttg
     7141 aagtcgtaac cggctttcaa gccgaagccc caagcgtcgg agttggtgct accgtcaacg
     7201 taagtaccgt tgctcatggt tgcagacagg tcgttgttga agtgagagta gctcaagcta
     7261 ccatcaacaa agacgttgtt cgcgaagtga gcagaagagt agatgtaggc agtctggctg
     7321 tcttgatcta cctgaccaga acggtcattc atgtcacctt tagcgaagcc tgcagccgca
     7381 ccgacgatcc acttagcgtt gttaccgtca actttggtat caacaccgac catgatgccg
     7441 ttaacatcct gatcatagtt gatggtgccg ttgtcgccgt tgaagttacc accgaagtag
     7501 cttacccatg cgccgccgtt atcagccagg ccatgacgag agttggtcag acgagtacca
     7561 acggtgtctt gttccaggtt ccagatattg gtgttcgcag acgggatgct cagcgccatg
     7621 ttagcgtagt cggtcagctc catctgttgc agaacaacgg tgttaccgcg ctgttcagcc
     7681 tgataggtgt atgcacccag gtcagctttg ttagcagcag agaaggtcgc gttggtgtta
     7741 gcgtcgttta cccgaataag ttctttatct ttgtaatcag cgatagtgcc agcgccagtt
     7801 gcgttgtcga tacgaacttt gtagttaccg gttgcagcaa caacgttatc aacagagctg
     7861 ttatccagtt cagtaccgtt gtctacgtcg ccgttaccat tgatagtcag gtgaccatca
     7921 gagttcatag caacaatacc gtaaccgtag ttagacttgg aagtatccca cgtacggtcg
     7981 ttcaccagat cagcgttcag aacgtaatcg ctgctgttga tgtcgaacac accagaaatt
     8041 acgttgccat tatggtcagc aatgttgccg ctggtcagtt ccagtttatc agtacggaaa
     8101 gcttcagtat cgaactgatc aacattcaca tccagcacac caccgttagt cacagtgata
     8161 gtattggtat acagattaga ataattattc caaccggtag attcggttaa attaacggta
     8221 ctgtagctat caacagtcag gtggtctgta gcaaccacgc catttgcacc aatattcagt
     8281 gaagaaccgt tggttaaacc aatggtatct gataccagag aggaatcagt aacatttacc
     8341 tgagagccat tatttacagc taatgtatca attactgaac tattaacggt attccattct
     8401 gaactattgt tcagtgctac gttaaatacg ccgctctgga atactttgtt gtcaataata
     8461 cggccccaat cattagcttc gatggtggca ccaggggtca ggctgttcgg cgcaacgtca
     8521 tacatatctg ccggagcaat tacttcagca ctcatgtttg ctgaaccaac ccacttacta
     8581 ccgttattca gagtaatatt cagttcgtca acgttctgag cgtcatctac ccaaccgccg
     8641 ttggtatcaa ggacgccgtc gccgttgctg tcatgaccgg tggaatagaa gaatacgcca
     8701 tcgttattcc agtgacttgt aaattcgaca tcacccaaca gagttgaatg gtcaaagttt
     8761 acattgtttt tcatggaata atttgagcca gcattgtcag tgaatgacag tgctacgtca
     8821 ttctggccac cattaccagc gtaatcactt ggttcagcag atttaccgaa gaagccatca
     8881 ctttcttcca gcggagtctg tgatcctgaa gttacagtgg aattagacac attgatgttg
     8941 ttgtcccact gatgatattc gctggtataa gttgtgttgc ttgtattgcc ttcagacagc
     9001 gcaatgccag ctacacggga gttgttggta atattgatat tgctttcaac atcaagattt
     9061 actgcaacac ccagagcggt atagaacgtt tcattggttg acttagattt atcaccatcg
     9121 agatgtgtgt cagtgaagta aagagcttca tagtcatcat caatggtgga attggatacg
     9181 ttcagagtga atacgtcacc atcgtaccaa ttgtagtcaa gatcagtagt agttccgtta
     9241 ttgaaagtat agccattata accttcaggc gtaatgctgt aataaccaaa tggcagttca
     9301 gaagtgattg acccatgaat tactgaattg ctgatatcta cctggttaac agaaccgcta
     9361 gcgtaatcat gattcaagta ataagtagaa atggtaccgt taacggtatc gcctttaaag
     9421 acggtatcga aatcgccatt ggcattgtta tagtaaacaa aaccattgta agttacgtag
     9481 ttaccatctg aacccatcgg gtccatgccg ccagcatcgg caattccatc attgtcggtg
     9541 tcatacggcc aaactaaatc ggtcgtggtg ttgtcatgat acccccaggt atcatagttt
     9601 gctgcattag cctgagaggt gatagccaga gtgcaagcca atgctaattg tgatactaca
     9661 agtttctttt tccaggagtg catttgtcat ccctcctcag ggacgtaata agttgatcca
     9721 tcaattatca atgcatagaa aacatattat gtttcgcgct aacgattatg ctgcgttaca
     9781 gacgaatggt tcaattat
//
LOCUS       DBANUU010000154         9675 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000154 DBANUU010000000
VERSION     DBANUU010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.156"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            38..1141
                     /locus_tag="ACIE68_003948"
     CDS             38..1141
                     /locus_tag="ACIE68_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFX5910191.1"
                     /translation="MKNSFDRARAAENTSKEAIEYLERASQMQAVMISQVSNDMRFSD
                     AFMLFTRLSLLITRRRPEIAVHCILIHVLPHIADVKVSDINRFMVNQLVNPLILDGKI
                     VMGRRVFSLMKQFLSWCAFQGMIDVSPLNDMSLNKVAGGAKPTPRERKLTDAEVWVFW
                     NIWDYFNVCAGTKWAARLCLVSARRPDEVLRAKKSEFNLKRGVWNQGKRNKSAREHSL
                     PLSSLMRTCIEELFEYGKDSQWLVPSNKKIGKDLPMSKVAIAQALRRILERPELMELE
                     PFTPRDLRRTARSYFPALGISQEVSRKIMNHSLEGIDRVYDRHDYMDEMRDALESFST
                     YIASIVEQPDLDEIDHKFKGDRLSTELIRVNFS"
     gene            complement(1136..1522)
                     /locus_tag="ACIE68_003949"
     CDS             complement(1136..1522)
                     /locus_tag="ACIE68_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFX5910192.1"
                     /translation="MSDNKIEFIESRYAAFIAGLIESSPMSQAQIAKTIGYKNANNLS
                     LIKSGKIPLPIDKVRPLALALGIEPSRLMMMVLEERQPELAAFLYKEGTAPLNEDEKQ
                     VLAAYNERFGKEKGASQKVVEAIKSL"
     gene            1774..1986
                     /locus_tag="ACIE68_003950"
     CDS             1774..1986
                     /locus_tag="ACIE68_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910193.1"
                     /translation="MNTTISSLIALEIGHVQKLADECVADILTDLPNEQIQVGVNDTT
                     GFIFELNNKRFTLLNTGSWSLAVRIC"
     gene            2085..4193
                     /locus_tag="ACIE68_003951"
     CDS             2085..4193
                     /locus_tag="ACIE68_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobT"
                     /protein_id="HFX5910194.1"
                     /translation="MSKKRSIKEVQDFRDSVKRVVALLSGKNIPVAERGDDAYVRYND
                     DGEPILVNIPSIPDNATPALMNAVRGFLDHEVAHILFTDIRVSNKMREKGRVPSWSLW
                     NALEDVFIERKMGQVFNGTRRNLMATQRLIIEKVFKPKASEAIAYCGKDQRALFLNFF
                     LCPVVRAWDGQAPFVDFMDEYWPVIEKPISLLKEHGIDVAVRNMSCTEDCVKVAATIA
                     KILKDTESESKGKESAPGKTSDPSDADQTDASGENNEDNEDHEAPSALDNHKSIKSES
                     HSKHKHDNNDSDDSDNSESSETIFDDTENDKEVSDSGASDNAASESFTTDHEKRKSTE
                     DGSSDIPTPSKMSLEEALEELDSMEDEVGGMTEDALSETIKSELTESSKSEYRPYNRS
                     YDFIGSIDQAEAHIKRLIKTFSDIDLGGYPISRYRIVPEGNQLFDKYIEKHLSSGVSS
                     TLAKDLERAIASRNRVQFIPGQRRGRIHGSSIYRLTMNDDRVFRKKEESKAVNACVQQ
                     VIDLSGSMSGETIKLALASAYTIADALDRINVPNIITGFTTFGSHMAAGELKAVKYEF
                     SRFESLMLPIIKNWNEKVNSREVRSRMGCVGYTFPLLNNVDGESIASLASLFSGRMED
                     RKIMLVLSDGAPWAVGRGFDAHLRSVAKQIETQTDIDLMAIGIMTDAPERFYSNHALV
                     TSVDSLGSSVVTELSRIILK"
     gene            4289..5524
                     /locus_tag="ACIE68_003952"
     CDS             4289..5524
                     /locus_tag="ACIE68_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFX5910195.1"
                     /translation="MTTTALQNEKNPSDYLVCKWCGKSFHYFKSHVANGNCEGIPESV
                     KDADPDTVLKMYTTQFPDEPTLSKKALDAIQAKRAEQKSEMAKSSGVTSSPGYTGTVE
                     YKTDLVAAHELLNVTVKELGTKRRTPLMVSVNVNTPFPEFVPEVKKGYVYGDFELIKD
                     IFMMLELGIPGYLWGHAGTGKSSLPTQLCALLNRPLIRAQHTASMEEAHVTGQILARD
                     GSTYFEPGLLALAMKHGWVYLADEYDFAFPQILGVYQPVLEGEALVIKEATPEWRRIT
                     PHERFAFIGTGNTNGSGDETGLYQGTNIQNAANFSRFGIVSNVKYMSKEAEINMLINA
                     GIVDEYAEKMVKFAGIVRDGYEEHLISQPIGPRELLLSAKIGMMRGDFVTGIERSFIN
                     KLPSASAQAAREVVQKIFG"
     gene            5705..9223
                     /gene="dnaE"
                     /locus_tag="ACIE68_003953"
     CDS             5705..9223
                     /gene="dnaE"
                     /locus_tag="ACIE68_003953"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFX5910196.1"
                     /translation="MKALMVRTDFSLGESALKAENAVKIAREAGYTAVISADSMNIAS
                     VIPLQRAAGDDMAVICGVKLNIVDDPTYEHRAKLAKESMRCMESLERGRNYSFTALIK
                     NEQGYRDICELMTAANTREQFYFVPRLSLEQLVSTYAKGNIILLTSDIGSVFQRNDFA
                     KIISTLITAGGKDNFYSVVYPHPTPFYDQINVRAMKVASALKIEPVAFYPAYYESIDD
                     ADIKDIAHMVTNNIKIDQPHRLRIPHQRDNAVNGRRHLLEALKAFSVRMDVPVTAAMA
                     STTQDTIIDACTWRWHELPPALPKMADDEPATLMKLAVAGLRKRLTTKEFGYTPPASE
                     NRVYVERLKYEMDTLTRLGFCGYFLMVRDLMNHSRETGIPVGPGRGSSAGSLVAWCIG
                     ITNVDPIRHGLLFERFINPERLDLPDADLDFSQARRHEVIEYLNERYGEDYVAGIPNF
                     TYLGAASALRDTARIYGVESADMAVSKELKNVEDDSLPLEELREQLASLDKYATKYPD
                     AFNAACKLQSLMRGFGRHAAGMIVAGVPLTERTPVERRGDARCIAFDKRYCEAMGLIK
                     LDVLGLATLDLLDSAKRYIKENTGEDINLDAISLEDRKVLDGFAAGYTQGVFQLESGP
                     MRKLLKDLGGGIEPMSFKTVVATTALFRPGPIQSGMLDDYVSVAKGFMTPESLHPVLD
                     ELTAETNGVILYQEQTMNATRLLAGFTMAEADAVRSAIGKKNMEKMKSMGEKFIVQAQ
                     AGWIDVELEDGTTQRIHRAEHFKCEDGTLKTVEEALEHGAKLPINAVRVTASHPGLSE
                     MKAKEIWTAFEKNGAYQFNKSHSVAYSLISYQSMWLKTHYPAEFFAAALTILGEDKHQ
                     GLVKDALTYGIRVLPPDVNVSSNRIEIRTLEDGSQALYAPFSAVKGCSENGCQAIMRA
                     REKVGGKFESVAQFDEAVEKRACNSRVRESLHKVGAFASIEPGSLPATDPERLRDQAE
                     LMGNLIIDAVKASRPFEMNPKRSAEINVLMTRMAAEMGLGEELIRPTIGIKPKIMIIL
                     DNANGNDARTGYFMENGYDDFKAKLLTVGDLRMGDLYVTGVCKKVKDKEKDYTKDEIG
                     QFTDFMREEINLVRPTYILTCGSRSTALFNNKSKPSDLIGRKEYFPELDATVFYGFNP
                     NILYFRPEEGERLEAILADIAETINK"
     gene            complement(9527..>9675)
                     /locus_tag="ACIE68_003954"
     CDS             complement(9527..>9675)
                     /locus_tag="ACIE68_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HFX5910197.1"
                     /translation="ADGSKDVFVHFSAIQSNNFRTLEEGQQVEFSIENGAKGPAAANV
                     VALS"
BASE COUNT         2742 a         2156 c         2318 g         2459 t
ORIGIN      
        1 cttatacagt gcatcggcgt aactcggtga ttgtcatatg aaaaatagct ttgacagagc
       61 acgcgctgcg gagaacacct caaaagaggc gatagagtat ctcgaaagag catctcaaat
      121 gcaggccgtt atgatctccc aggtcagcaa tgacatgaga ttctcggacg cattcatgtt
      181 attcactcgc ttatctctgc tgataaccag acgtcggcca gagatcgctg ttcattgtat
      241 tttgatacat gttttgccgc acattgccga tgtaaaagta agtgacatta ataggttcat
      301 ggtgaaccaa ctggtcaacc cactaatact ggatggcaaa attgttatgg gccgccgcgt
      361 tttctctctg atgaagcagt tccttagctg gtgcgccttc caggggatga tagacgtgtc
      421 accgttaaac gatatgtcac ttaacaaagt tgccggtggc gcaaagccca cacctcgcga
      481 gcggaagctg accgacgcag aggtatgggt gttctggaat atatgggact acttcaatgt
      541 gtgtgctggt acaaaatggg cggccaggct atgtcttgta tccgcaagac gacctgacga
      601 agtactgcgg gctaaaaaaa gtgagttcaa tcttaagcgt ggggtttgga atcaaggcaa
      661 gaggaacaaa tctgcccgtg agcattctct gcctttaagc tcattaatgc gcacttgtat
      721 tgaagagttg ttcgaatatg gtaaagacag ccagtggctc gtgccttcga ataaaaaaat
      781 cgggaaagac cttcctatgt ctaaagtggc aatagcccag gcattacgtc gtattctgga
      841 acgaccagaa ctgatggagc ttgagccatt tacaccccga gatttgcgcc gtactgcgcg
      901 tagttacttc ccagcattag gcataagcca ggaggtatca cgtaaaatca tgaaccacag
      961 tcttgagggg atagatcggg tctacgaccg gcacgattat atggacgaga tgcgagacgc
     1021 cttagaaagt ttctcgacgt acatcgcatc aatcgtagag caaccggatt tagacgaaat
     1081 tgaccacaaa tttaagggag atcgtctatc aacagagctt attcgtgtaa atttttcata
     1141 gagactttat ggcctcaaca accttttgtg atgcgccttt ctctttaccg aatcgctcgt
     1201 tatatgcagc aagaacctgt ttttcgtcct cgttaagagg agcagtgcct tctttgtata
     1261 aaaatgctgc gagttcgggt tggcgttctt ccagcaccat catcataaga cgacttggct
     1321 caatgcccag tgccagcgcc agcggacgaa ccttatcgat aggcaaagga attttgccgc
     1381 ttttaattaa agaaaggttg ttggcgtttt tatacccaat tgttttggct atctgggcct
     1441 ggctcatagg tgaggattca atcaaccctg cgataaaagc agcgtagcga ctttctataa
     1501 attcaatctt gttatcagac atggttacaa cctttgcgcg ttcaattctc tctggtaagt
     1561 gcttaccgat attacatcaa aggttagggt tgtaaagcta ttatcatttt tttcgatagg
     1621 cacttaaaag accggttaaa ggccattgca cggagaaaaa ttagcccaaa acaggtaaga
     1681 aaatcaactt gcatatgata tgaatgtatt cagtattgat acaaatttta gtagtattcc
     1741 ttaccatagt ataagttaga atggattgat tgaatgaaca ccactatttc cagcctaatc
     1801 gctcttgaga tcggacacgt acagaaatta gctgatgagt gtgtagctga catcctcacc
     1861 gatctaccga atgagcagat tcaggttggt gtgaatgaca caactggctt tatattcgaa
     1921 cttaacaaca aacgcttcac gcttctcaat accggctcct ggtctttagc cgtcagaatc
     1981 tgttaacccc tcttctccct gcgcgaatgg cttagttccc tgttcgcgca gtgctacatt
     2041 aaacacacta gtaaataatt tgttttcata acaaaggatt agccatgtct aaaaaacgtt
     2101 ccatcaaaga ggttcaggac ttccgtgaca gtgtaaaacg agtagtcgct ctcctttcag
     2161 gtaaaaacat ccctgttgca gaacgagggg acgacgctta tgtacgctat aacgatgatg
     2221 gagagccaat tctcgtaaac atcccatcaa tcccggataa cgcaacaccg gcattgatga
     2281 atgctgtgcg cggatttctc gatcatgagg ttgctcacat tttgtttacc gatattcgtg
     2341 tgtccaacaa aatgagagaa aaaggacgcg ttccttcctg gtcgctatgg aatgccttag
     2401 aagacgtgtt catcgagcga aaaatgggtc aggtctttaa cggaacaaga cgtaatctga
     2461 tggcaactca gcgccttata atcgaaaaag tctttaaacc aaaggcttca gaggctattg
     2521 cttattgtgg caaagatcag cgcgcgcttt ttctaaactt ctttctctgt ccggttgtaa
     2581 gagcctggga tggccaagca ccgttcgtag atttcatgga tgaatattgg cctgtcattg
     2641 agaaaccaat ttcattatta aaagaacatg gtatcgatgt ggccgtgcgt aacatgtctt
     2701 gcaccgagga ttgtgtaaag gtggctgcga ccatagctaa gatcctcaaa gacactgaaa
     2761 gtgaaagcaa aggtaaggag tcagctccgg gaaaaacttc cgatccttca gacgctgacc
     2821 agacggatgc ctctggagaa aacaatgaag acaacgaaga tcatgaggca ccctcagcgt
     2881 tagataatca caaatctatc aaatcagaat cacacagtaa gcacaaacat gataataacg
     2941 acagtgatga ttcagataat tctgaatcat cagaaacaat attcgatgat acagaaaatg
     3001 ataaagaggt atcagattct ggtgcttctg ataacgcggc gtcagaatca tttaccactg
     3061 accacgaaaa aagaaaatcg acagaagacg gctcttcaga cattccaact ccgtcaaaaa
     3121 tgagtctgga agaggcttta gaggagttgg atagcatgga agatgaagtc ggaggcatga
     3181 cagaagatgc gctatccgaa acgattaaaa gcgagttaac agaaagctcg aaaagcgaat
     3241 acaggccata caatcgctca tacgacttca tcggctcgat tgatcaggca gaagcccata
     3301 tcaaacggct tattaaaaca ttctccgata ttgatttagg tggatatcca atcagccgtt
     3361 atcgcatcgt tcctgaaggc aaccaactct tcgacaaata tattgaaaag cacctttcgt
     3421 caggtgtttc gtcgacgctg gcaaaagacc tggaacgtgc aatagcaagc agaaacagag
     3481 ttcagtttat acctggccag cgtcgggggc gcattcatgg ttcaagtatc tacagattaa
     3541 caatgaatga tgatcgcgtg tttcgtaaaa aagaagaatc taaagccgtt aacgcctgtg
     3601 ttcaacaagt gattgattta tcgggttcaa tgagtggcga aacgataaaa ctagctcttg
     3661 caagtgcata taccatcgcc gatgcacttg atcgaataaa tgttcccaac attatcaccg
     3721 gcttcactac atttggtagt catatggcgg caggagaact taaggctgtc aagtatgagt
     3781 tctctcgctt tgaatcttta atgctaccta tcatcaaaaa ttggaatgaa aaggtaaatt
     3841 ctcgcgaagt tcgctcacgt atggggtgcg taggctacac attcccactt cttaataacg
     3901 tggatggtga aagcatagcc agccttgcat cgttattttc cggtcgcatg gaggacagga
     3961 agatcatgct tgttctgagt gatggcgcgc cgtgggctgt tgggagaggt tttgacgctc
     4021 atttgcgttc agttgcgaag caaattgaaa cgcagactga tattgatttg atggcaattg
     4081 gcatcatgac tgacgcaccg gagagatttt actcaaatca tgccctggta acgagcgttg
     4141 atagtcttgg ttcatctgta gttactgaac tatctcgtat cattttaaag tgaacaaaac
     4201 agccttaacg ataagtaacc acttacgata gatgatgata tatttatata agaagttgaa
     4261 cgctcattag aaaacaaagg aaaaacgcat gactactact gcactgcaaa atgaaaaaaa
     4321 tccttctgat taccttgttt gcaagtggtg cggaaaatca tttcactatt ttaagtccca
     4381 tgtagccaat ggtaattgcg agggcattcc tgagtcagta aaagatgccg atcctgacac
     4441 cgtactgaaa atgtacacaa cacagtttcc agatgagcca acgctatcga aaaaggcact
     4501 tgatgcaatt caagctaaac gtgccgagca aaaaagcgaa atggccaaat catctggcgt
     4561 gaccagtagc ccaggctaca caggcacagt tgagtacaag acagatctgg tcgcagctca
     4621 cgaactgcta aatgtaacgg tgaaagaact cggaacaaaa cgtaggacgc cgctcatggt
     4681 tagcgtcaac gtcaatacgc cgtttccaga gttcgttcca gaagtgaaga agggatacgt
     4741 atatggcgac ttcgaactga tcaaagacat tttcatgatg cttgaacttg gcatacctgg
     4801 ctatttgtgg ggtcatgcag gaacaggcaa atcttcattg cctacacaac tatgtgcttt
     4861 gctcaatcgt ccgttaatcc gtgcccaaca tacagcatca atggaagagg ctcatgttac
     4921 gggacaaatt ctggcgcgtg atggctctac gtatttcgag cctggcttgc ttgcgctcgc
     4981 aatgaagcat ggctgggttt acctcgcgga tgaatacgac tttgcgtttc cacagattct
     5041 tggcgtgtat cagccagtgc tggaaggtga agcgttggtc atcaaagagg cgactccaga
     5101 atggcgtcgc attactccgc atgaacggtt tgctttcatt ggcactggca acacgaacgg
     5161 atctggtgat gaaaccggct tgtaccaggg tacaaacatc cagaacgccg cgaacttttc
     5221 gcgttttggc atcgtttcga atgtgaaata catgagcaaa gaggcagaga tcaacatgtt
     5281 gataaatgcc ggtatcgtgg atgaatacgc agaaaagatg gttaagtttg ccggtatcgt
     5341 tcgcgatgga tacgaagaac accttatcag tcagccaatt ggccctcgtg aacttttgtt
     5401 gtcggccaag attggaatga tgcgaggcga ctttgtgaca ggtattgagc gttctttcat
     5461 taacaaactc ccttcagctt ctgcacaagc ggctcgtgaa gttgttcaaa aaatatttgg
     5521 ttaatcgtgc gtaaaggatg tttcggctct cttatcgctg cttctgaaac tggtaaggct
     5581 tgtctggtgt gtccagacaa gcccgattgt caccaatcag caaaagaagt tgcgatttcg
     5641 atgcatggga aattcgtagg cttccccaat gacaaaatca aaaaaaccag aaaggtaaaa
     5701 acacatgaag gcactgatgg ttcgaactga cttctcactt ggggagtcgg ctctaaaagc
     5761 agaaaacgcg gtgaagattg ccagagaagc tggctacacc gctgtaattt cagcagatag
     5821 catgaatatt gcgagcgtta ttccactaca acgtgccgct ggtgacgaca tggcggttat
     5881 ttgtggtgtg aaactaaaca ttgttgatga tcccacatac gagcaccggg ctaaacttgc
     5941 taaagaatct atgagatgta tggaatcatt agagcgggga cgtaactact cgtttaccgc
     6001 tctaattaaa aatgagcaag gatatcgcga catctgcgaa ctaatgacgg cggccaacac
     6061 acgagaacag ttctactttg taccgcgtct ctcgctcgaa cagttggttt ctacatatgc
     6121 caaaggcaac atcatcctgc ttacttccga catcggtagc gtgttccaac gcaacgattt
     6181 tgcaaaaatc ataagcacac tgattacagc gggtggaaaa gacaacttct atagtgtggt
     6241 ttatccgcac cctaccccat tctacgacca gattaacgtc cgggcgatga aagtcgccag
     6301 cgcattgaaa atagagccag tggcgttcta tcccgcttat tacgaatcga tcgacgatgc
     6361 agacattaaa gacattgcgc acatggttac gaacaacatc aaaatcgacc agccgcatcg
     6421 tctgcgtatc ccccaccagc gagataacgc cgtcaatggt cgccgccatc tccttgaggc
     6481 gcttaaagcc ttctccgttc gcatggatgt gccggtaaca gctgcaatgg cctcaacaac
     6541 gcaggatacc attatcgatg cctgcacatg gcgctggcat gaattgccgc cagcactgcc
     6601 aaagatggca gacgacgagc ctgcaacgct tatgaaactg gctgttgcag ggctgcgtaa
     6661 acgtcttacc acaaaagagt ttggctacac accacctgct tctgagaaca gggtttatgt
     6721 tgagcggcta aagtacgaaa tggacacgct gactcgcctg ggattttgtg gttacttcct
     6781 gatggtacgc gatctgatga atcatagtcg tgaaactggc attcccgttg ggcctggtcg
     6841 tggttcctct gccggttctc tggtggcgtg gtgcataggc ataaccaacg tcgacccaat
     6901 ccgtcacggt cttctgtttg agcgtttcat caaccctgag cgtctcgact tgccggatgc
     6961 ggacttggac ttcagccagg cacgtcgcca tgaggtgatc gagtatctga atgaacgcta
     7021 cggcgaagat tacgttgcgg gtattccgaa cttcacctac ctgggcgcag cctctgcact
     7081 acgtgatacc gctcgtattt atggtgtgga gtccgcagat atggcggtat caaaagaact
     7141 gaagaacgtc gaggatgata gccttccatt ggaagagctg cgcgaacaac tggcaagtct
     7201 cgacaaatac gcaacaaaat atcctgatgc attcaatgca gcctgcaagt tacaaagcct
     7261 tatgcgtggc tttggtagac atgcggcagg gatgatcgta gcaggtgttc ctctgacaga
     7321 acgtacaccg gttgagcgcc gtggtgacgc gcgttgtatc gcatttgaca agcgttactg
     7381 cgaggctatg ggcctaatta agctggacgt gcttggcctg gcaactctcg atttgctcga
     7441 tagtgcaaaa cgctacataa aagagaacac aggtgaagat atcaatcttg atgccatttc
     7501 tcttgaagat cgcaaggtgc tggatggttt tgctgctggg tacactcaag gtgttttcca
     7561 gcttgaatca ggcccaatgc gcaagctgct taaagattta ggtggtggaa ttgagccaat
     7621 gagctttaaa acggtcgtcg ctacaactgc gctcttccgg ccggggccaa ttcaatcagg
     7681 catgttggat gactatgttt ctgtcgccaa aggctttatg acgccggaat cattacaccc
     7741 cgttcttgat gaacttaccg cggaaacaaa tggcgtgatt ctctatcagg aacagacgat
     7801 gaacgcgact cgattgcttg ccggcttcac aatggctgaa gctgacgctg tgcgttccgc
     7861 aatcggtaag aagaacatgg aaaaaatgaa gagcatgggc gagaagttca tcgttcaggc
     7921 tcaagctggc tggatagacg ttgagctgga agatggcact acacagcgca ttcaccgtgc
     7981 ggaacatttt aaatgcgaag acggaactct gaaaactgtc gaagaggcac ttgagcacgg
     8041 cgcaaaacta cctataaacg cagtacgcgt tacagcgtca catccagggc tatcagagat
     8101 gaaagcgaag gagatctgga ccgcatttga gaaaaacgga gcctatcagt tcaataaatc
     8161 acactccgtt gcttattctt taatcagtta tcagtctatg tggctaaaga cgcactaccc
     8221 tgctgagttc ttcgcagctg cgctcaccat tctgggcgag gataagcatc aggggctggt
     8281 gaaggatgcg ctgacctatg gcattcgcgt attgccacca gacgttaatg tgtcatctaa
     8341 ccgaattgag atccgcacac tcgaagacgg cagtcaggca ctgtatgcgc cattctctgc
     8401 tgtgaaaggc tgttctgaaa atggttgtca ggcaattatg cgtgcgcgtg agaaagttgg
     8461 tggcaaattc gagtcagtgg cacaattcga tgaagcggtc gagaagcgtg catgtaacag
     8521 tcgtgtacgc gagtcgcttc ataaagtagg ggcttttgcg tcaattgagc caggcagtct
     8581 gccagcaact gatcctgaac gactgcgcga ccaggctgag ctgatgggca atctcatcat
     8641 tgacgctgtt aaagcatcac gtccgttcga aatgaatcct aagcgttctg ccgaaatcaa
     8701 cgtactcatg acacgtatgg cggctgaaat gggcttgggt gaggagttga tacgcccgac
     8761 tattggtatt aaacccaaaa tcatgatcat tctggacaat gcgaacggca atgacgctcg
     8821 taccggctac ttcatggaga acggatacga cgactttaag gcaaaactac tgacagttgg
     8881 agatctgcgc atgggcgatc tttatgtcac gggtgtttgt aagaaggtta aggacaaaga
     8941 gaaagactat accaaagacg agataggcca gttcacagac tttatgcggg aagaaattaa
     9001 tcttgtacga ccaacctaca ttttgacgtg tggtagtcgt tcaaccgcac tatttaacaa
     9061 taagagtaaa ccatcagatc tgattggtcg taaggagtac ttcccagagc ttgatgcaac
     9121 cgtcttctac ggatttaacc cgaatatcct gtacttccga ccggaagaag gagagcgact
     9181 ggaggccatt ctggctgata tcgcggagac aataaataag taataaagaa aacccgcctg
     9241 ttggcgggtt tataaagaat tatggcgctt gttgaggaag tcactcctct tacgcacttt
     9301 gttttgccat gccggcagtt agcttctgcc tttgactatt catgcggcaa ccccgcattt
     9361 cgccacaatg ggcaattcac ttttatggaa taaactggcc gttgtgtcga tttaattagc
     9421 atggccttac catgctaatt tattcaactt gtacaatcct acaaatctat ccactcaggc
     9481 ttacaccgtc ttcgttattg tagaaatgag gatcatcagc gttgaatcag cttagagcaa
     9541 cgacatttgc tgctgctggg cctttagccc cattctcgat agagaattcg acctgctggc
     9601 cttcttccaa agtgcggaaa ttattactct gaattgccga aaaatgtaca aaaacatctt
     9661 tactgccatc agcag
//
LOCUS       DBANUU010000155         9632 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000155 DBANUU010000000
VERSION     DBANUU010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.157"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            82..2760
                     /locus_tag="ACIE68_003955"
     CDS             82..2760
                     /locus_tag="ACIE68_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECIAI39_1904 family autotransporter adhesin"
                     /protein_id="HFX5910198.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHEEHKHAVAIKDGEVK
                     NGEVKGGEVKYIDNALVITTGNGSYGVSVAGVGSNLTINNGVIVTTGGLYPSDNSSLS
                     TALTASAIVSEDGGAVTLRGNNTIVTTGDYSVGLLSQVDGNLNTDTIIRVNPDGSATP
                     SFSDGYNTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTENNENSAKAILDMA
                     KGEITTHGTESYAAYANGTVVKAGDKLDYTNASVTLTDVDITTHGDNAHAIAARQGTV
                     SFNQGEIYTTGPDAAIAKIYNGGAVTLKNTSAVAHQGAGVVLESSINGQEATVDILSG
                     SSLRSANEILYNKNETSNVTITDSEVSSAADVFINNIKGHLTVDATDSKITGSANLST
                     DDNTHTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDFEPTRLTITENYVGN
                     NGVLHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESN
                     GEFIKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPIVAPTPV
                     LRPEAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRD
                     SNGQLRTTSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGS
                     VRGYSAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEA
                     GYGFALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLG
                     FRTFIRTQEKNSGPHGDDFEPFVEMNWLHNSKDFAVSMNGVKVEQDGARNLGEIKLGV
                     NGNLNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(2816..3151)
                     /locus_tag="ACIE68_003956"
     CDS             complement(2816..3151)
                     /locus_tag="ACIE68_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFX5910199.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(3155..3499)
                     /locus_tag="ACIE68_003957"
     CDS             complement(3155..3499)
                     /locus_tag="ACIE68_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFX5910200.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(3501..3674)
                     /gene="ymgI"
                     /locus_tag="ACIE68_003958"
     CDS             complement(3501..3674)
                     /gene="ymgI"
                     /locus_tag="ACIE68_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFX5910201.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3775..3960
                     /gene="ymgJ"
                     /locus_tag="ACIE68_003959"
     CDS             3775..3960
                     /gene="ymgJ"
                     /locus_tag="ACIE68_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFX5910202.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKVSVRQYYQKRVIFYPTTFQR
                     TAFKKCLFLMRDIIISS"
     gene            3921..4040
                     /locus_tag="ACIE68_003960"
     CDS             3921..4040
                     /locus_tag="ACIE68_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFX5910203.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(4519..4785)
                     /gene="minE"
                     /locus_tag="ACIE68_003961"
     CDS             complement(4519..4785)
                     /gene="minE"
                     /locus_tag="ACIE68_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFX5910204.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(4789..5601)
                     /gene="minD"
                     /locus_tag="ACIE68_003962"
     CDS             complement(4789..5601)
                     /gene="minD"
                     /locus_tag="ACIE68_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFX5910205.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(5625..6320)
                     /gene="minC"
                     /locus_tag="ACIE68_003963"
     CDS             complement(5625..6320)
                     /gene="minC"
                     /locus_tag="ACIE68_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFX5910206.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            6840..7208
                     /locus_tag="ACIE68_003964"
     CDS             6840..7208
                     /locus_tag="ACIE68_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFX5910207.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(7328..7729)
                     /gene="pliG"
                     /locus_tag="ACIE68_003965"
     CDS             complement(7328..7729)
                     /gene="pliG"
                     /locus_tag="ACIE68_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFX5910208.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            7971..8264
                     /locus_tag="ACIE68_003966"
     CDS             7971..8264
                     /locus_tag="ACIE68_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFX5910209.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            8336..8995
                     /locus_tag="ACIE68_003967"
     CDS             8336..8995
                     /locus_tag="ACIE68_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFX5910210.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIIPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            9087..9533
                     /locus_tag="ACIE68_003968"
     CDS             9087..9533
                     /locus_tag="ACIE68_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFX5910211.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRDNLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT         2560 a         2123 c         2313 g         2636 t
ORIGIN      
        1 ttaccctccg ttttggtaat ctgagctcat aggttatttg accaggaatg tttgttagtc
       61 caatgaatgg atggttaatc tatgaaactg aaaaaactcc ccgggtttag tttaggactt
      121 attgctctgg cggtgggtaa tgcatatgct gaacagttta ttcagcacga ggaacataaa
      181 catgctgtgg caataaaaga tggagaagta aaaaatggag aagtaaaagg tggagaagta
      241 aaatatattg ataatgcgct agttataaca acgggtaatg gaagttacgg ggtatccgtt
      301 gcaggagtgg gtagcaattt aaccattaac aatggcgtca ttgtaacaac gggcggttta
      361 tatccttctg acaatagttc tctaagcaca gcattaacag ctagtgcaat cgtaagtgaa
      421 gatggcggtg ctgtcacttt gcggggtaat aatacgattg tcacgacggg agattattct
      481 gttggtttgt taagtcaagt tgatggaaac ctgaatactg ataccataat aagagtcaac
      541 cctgacggtt cggctacccc ctctttttct gacggatata atacttttat tgttactgcg
      601 ggtaatcacg cagtcggcgt tcttgcgtgt gcatctcccg gaagcgctcg tgcgtgtgta
      661 tcttctcttg atgaagaaag tactgccgat acagaaaata atgaaaatag cgccaaagca
      721 atactggata tggcaaaagg ggagataaca acccacggaa cagaaagtta tgccgcttac
      781 gctaatggca ctgtcgtcaa ggctggtgac aagcttgatt ataccaatgc cagcgttaca
      841 ttaaccgatg ttgatatcac cacgcatggg gacaatgctc atgccatcgc tgctcgccag
      901 gggacagttt catttaacca gggagaaatt tatacaacgg gtcctgacgc cgcgattgct
      961 aaaatttata atggcggcgc ggtgacgctg aaaaatacat ctgcagtcgc gcatcagggg
     1021 gctggagttg tgctggagtc ttccataaat ggtcaggaag caacggtaga tattttatct
     1081 ggtagctcac tacggtcagc aaatgaaatc ctctacaata aaaatgagac gagtaacgtg
     1141 accattactg atagtgaggt atcatcggct gcagatgttt ttatcaataa tattaaaggt
     1201 catttgaccg tcgatgcaac tgattcaaaa ataacgggtt cagccaatct ttcaacagat
     1261 gataacactc atacctatct gtcgctttca gataatagta cctgggatat taaagctgac
     1321 tcaacggtga gcaacctcac ggttgacaat agtacggtct atatttcccg ggcagatgga
     1381 agggatttcg aaccaacaag attaacaata actgaaaatt acgttggtaa taatggcgta
     1441 ttgcatctca gaactgaatt gggtgatgat aattcagcta cggataaagt cgttattaat
     1501 ggaaatacct ctggaacaac ccgagttaaa gttaccaatg ctggcggcag cggggcttac
     1561 acgttaaatg ggatagagat tatcagcgtt gagggggaat caaatggaga atttattaag
     1621 gattcgagga ttttcgccgg tgcctacgaa tattcattaa cccgaggtaa taccgaagcg
     1681 accaataaaa actggtatct gactaacttc caggcaacga gcggcggtga aacaaactcc
     1741 ggaggaagtt cagcgcctat tgttgcgcct acccccgtcc tgcgccccga agctggaagt
     1801 tacgtcgcca acctggcagc cgctaacact ctttttgtta tgcgtctgaa cgatcgtgca
     1861 ggtgaaacgc gttacatcga tcctgtaact gaacaggagc gttcaagccg actttggcta
     1921 cgtcaaattg gcgggcataa tgcctggcgt gacagcaacg gacagttgag aacgacctcg
     1981 catcgctacg tctcgcagtt aggggccgat ctgttaaccg gtggttttac cgacagtgac
     2041 agttggcgtt tgggagtgat ggctggttat gcccgcgact acaactcaac tcattccagc
     2101 gtgtcggatt atcgttcgaa agggagtgtc agaggctata gcgcagggct gtatgccacc
     2161 tggtttgccg atgacatcag taaaaaaggc gcatacattg acgcctgggc gcaatatagc
     2221 tggtttaaaa actcggtgaa aggggatgaa ttagcctatg aatcctatag cgcgaaaggc
     2281 gcaaccgtct cgctggaagc gggttacggc tttgccctga ataaatcctt tggtctggaa
     2341 gcggcgaaat atacgtggat cttccagcca caggcacagg ctatctggat gggcgtcgat
     2401 cataatgcgc acacggaagc caatggctca cgtattgaga atgacgcaaa taacaacatc
     2461 cagacccgac ttggtttccg cacctttatt cgtactcagg agaaaaacag cggtccgcac
     2521 ggtgacgact ttgaaccttt tgttgaaatg aactggctcc ataacagtaa agattttgct
     2581 gtctcaatga acggtgtgaa agtcgaacaa gacggggcgc gtaatttggg ggaaattaaa
     2641 cttggcgtaa atggcaatct gaatccggcg gccagcgtct ggggtaatgt gggcgtgcag
     2701 ctgggtgata atggctacaa tgacaccgca gtgatggtgg gcctgaaata taagttctga
     2761 tccccgttag ctaaaatacc gcgtcgaatt catcgacgcg gtaacatatt aaatattatt
     2821 ttttgccgat agcacgcatg gtgtcatcaa ttgccgtgat caacagcatt tgcgggtctt
     2881 tagcgcaaac ctgattcagt ttttctacca ctttggcgct cagttccgga gattgctcaa
     2941 tttttaaatc acggctggca acgctggcat tacccattac cgcaacaatt tctgcaacct
     3001 gtgcgctgtc agtttttgcc atttcgttgg cttctgcgca agtaatatag gtttctgacg
     3061 gcaaaccgtt tttaatattg tagtcctgcg cccaggtcat tggtgcgaaa acaaacaggc
     3121 ccgccagtaa agcaaatttt ttcatcatca ttccttattt cattttaccc agaattgcac
     3181 cacccgtacc gccaatcacg gcacctttaa tcgccccttc gaggccattg ccggtcagaa
     3241 cgccagtgac agcaccaacg gcggcaccta cttttgcacc tttacgcgca tttttaccgt
     3301 cgcggccttt ttctgttact gcaccaacac cagcgccaac agctgcacct ttcagtacgc
     3361 cattaacacc attgccagta agtaaaccaa cgcctgcgcc tagcaatgca cctttcgtgg
     3421 tgcgattcat atccgccatc gctggcgtgg agcagaacaa tgctgagata agcccgaagg
     3481 caagtatttt tttcttcaac ttagatgtcc ggtattaagt aagttgcaca cacaataatt
     3541 tcgtcttcaa ttaagatctg cttaactaaa gaacgctcgc tattattcag ataattcaaa
     3601 atgagcgtgg ctgtgatgat aggaattatg ttttttacgt gaatgagaat aatcttaaat
     3661 gaggaataac tcattgattg acaatatttt tattcaagaa gtgtcattga ctgttaacgc
     3721 aatgttgtaa aggtaagata atctgattta tcaatattat tgtgtgattt ttatgtgagc
     3781 agaagatatt catcagcaac gattacatta gtcattttat tttgccgacg gcctcattgt
     3841 cgaaaagtca gcgtacgaca gtattatcag aaaagagtga ttttttatcc aactacattt
     3901 cagcgcactg cgtttaaaaa atgcctcttt cttatgcggg atatcatcat ttcatcatga
     3961 tgtctttggt gagcggtgaa cacaatacac ctgcgctgtc tcttcaggat gaatcccctg
     4021 gtctggtgcc tgcgggctga tgttgcagcc gagctaaggt cacttagacg ctactatcat
     4081 ttatcccatg gcatggagtc aaaatcagtc gatacccgca gtatatatcg tgaactggtc
     4141 gttctgacca tcaacgatta aactgcgctt cggcacagtt ttcgtttaca ggatgttaaa
     4201 agagaaaatt ctggggcaaa aaaagcccgc cagttacggc gggaaacctc atcctatggg
     4261 agaacaatga ataatgaaat tgcggggtta tcatctccca gtgtatccac actaacaata
     4321 aggttattta ctcagccagg cataaacatt ttgttttgtg cgtgggaaca gccttaaggt
     4381 gtaaaggggg aggtggaaat agcaatgagg agtatcagca agaatactcg ccgctttacc
     4441 acaacgtgga tgagagggat gaaaaactca aggcagagat aactctgcct tgaagataaa
     4501 tgcgctttta cagcgggctt atttcagctc ttctgcttcc ggtaaggtca cgttcagctc
     4561 aagaatagaa atatcgccat ctttttgctc aagctgtacg gttaccatct caggatcaat
     4621 ttgtacgtat ttacaaatga cctcaagaat atctttacgc aactgcggca gataatgcgg
     4681 ttctgcatcg ctgcgacggc gttcagcaac aataatctgc agccgttctt ttgcaatgtt
     4741 ggctgtgttt ttcttccgcg agagaaagaa atcgagtaat gccataactt atcctccgaa
     4801 caagcgtttg aggaagcctt tcttctcttc ttcaatgaag cggaaaggac gttcttctcc
     4861 caacagacgt tctacggtat ctgcgtaggc tttacccgca tcggcgttaa tgtcgagaat
     4921 gaccggttca ccctggttag aggcgcgcaa tactgattga tcctctggga tcacgccgac
     4981 gagtttgatg cgcaggatct ccagcacatc ttccatgctc agcatgtcac ctctgcttac
     5041 gcggcctggg ttatagcgcg ttaacagcag gtgctcttta ataggctctt cgccattttc
     5101 tgcgcggcgt gattttgacg ccagaatgcc taaaatacgg tcagagtcgc gtactgagga
     5161 gacttccggg ttggtggtaa taatggcttc gtctgcaaaa tagagtgcca ttaacgcacc
     5221 ggtttcaatc cctgccgggg agtcacaaac gataaattca aaatccatcg ctttcagatc
     5281 atcaagaact ttggcgaccc cttcacgggt gagggcatct ttatcgcgtg tttgcgatgc
     5341 cggcagaata tagagatttt cagtacgctt atctttaatt aacgcctgat ttagcgttgc
     5401 atcgccctga atgacgttga cgaaatcgta aacgacccgg cgttcacaac ccataatcag
     5461 gtcgagatta cgcaggccga tatcaaaatc tatcacgaca gttttctttc ccttctgggc
     5521 caaaccagtg gcgatggccg cgctggaggt tgtcttacca acaccccctt tgcccgaagt
     5581 aacaacaata atgcgtgcca tagaaattcc ttgttaaaaa gggatcaatt taacggttga
     5641 acggtcaaag cgttttcgac taactgcagt cgcgccgctt tgccataaaa ttctgctggg
     5701 atttgatcac tcagccagta ttcacctgcg atggacacca gttccgccat caggttcgta
     5761 caaaatattt gcgtttcccg gtcaccactt gcccctgcca gcgcacgacc gcgcatcatg
     5821 ccatagacat gaatgttacc atcggcaatt aattcggccc cagcgctaac gtggcttgta
     5881 acaatcagat cacattgtgg agcataaata cgctgaccgg aacgcaccgg ggtatctatt
     5941 aaacgcgttt ttgtgaccgg cgttgtattt tgcgctggag cctgcggtgc gggagctgga
     6001 cgtggcgctt tttcctttcc ttccgtcagg ataggcagcc ccattttttc aatttcggct
     6061 ttaagttgcg catctttgca gccacttacg ccaataaccc gcaaaccggt tgccgaaacc
     6121 gccttatgca tcgctgacca gtttaccggg tcttccagtg cactgacgtt gagtacaacg
     6181 ggggcatgtt ttaaaaatgc gggggcctga gcgattttgt cttccagcgc ctgatggata
     6241 accttaggtt ctgcctcatg cagatgaacc acagataaag tgaagctact gcctttaagc
     6301 tcgattggcg tgtttgacat cctggcctta ctcaattagc tattaatcat cgccagcgcg
     6361 cgatgatgtt ccgaagacta taaggcatgt tatagtctgg attatattga ggcaagtcac
     6421 cctcccattt attcagagta aaagtctatt ctgtgataaa tggcgctgat tcatagctta
     6481 aaaaataccc ttgtcaatca acccattgcc gtcgtacttt tgattcttct tatttacgct
     6541 tcctttttcc gcaccctaat cgtgacagca tctccagacc tgtttaaata tccagtattg
     6601 aggcaagtgt attttttcac cacgccaggc tttcttaact ttccattttt tgcggcatca
     6661 ttaaagaaca gatacccgtt atcttaatat ccgccacaga attgctctat catagaaacc
     6721 gcagaagacg tctgatactt ctgtcaggtc atcgtaactc gtccaggtca gcggttttga
     6781 ttacggattg atgatgaaat gagcataagt gttgtacggt tggattatca ggatgtgcta
     6841 tgaatatgat gcgtattttt tatatcggat tgtcaggtgt ggggatgatg ttctcatcaa
     6901 tggcgtctgg aaatgacgct ggcggacttc aatctccggc gtgcggcgtt gtttgcgacc
     6961 cgtatatttg tgtgaactca gatggcattt ccccagagtt aacaaggaaa tatctcggcg
     7021 aaaaagccgc tgaaaactta caatcattac aaggctacga tcccagcgaa tttacattcg
     7081 ctaacggtgt attttgcgat gttaaagaaa aattatgtcg tgatgatcgt tattttggtg
     7141 tagatggtaa gcggagtgga aaaatcaatc aaaccaccac aaagatgtta tttatgtgtc
     7201 gtgagtgatg ccttgtttac attatgttct attcggcatt gtctgctatt cgtcattaac
     7261 ctcttgagcg acagagagga gtggggcgtt aacgtttatg acggggagag tccccgtcgg
     7321 ttgacattta cttaatttga atatcgacgt tatatttttt agttttgttt ttacgggcgt
     7381 cgtttcgggt ttgcagcact cgcagctcat atttgccaga agcagggagt gagtattgtc
     7441 cgtgactatc gagttctggg gaatatctgg ataaatcaac agaatcatcg attcctggcc
     7501 cgaagaggta agtatcagcg ccttcattag agatactcac atgcactttc tgaccttttt
     7561 tggcatagaa ggtatatgta tcgtaatcgt atccctttat ttcgccggag tactgagcgc
     7621 tgctatggcc ttttctgaac tcaacattga cattcttacc cgcagcaaag ctcgtggagg
     7681 ttagcagtgc aagcagcaat acagccttac tgatgctctt gattttcatt ggtagtctcc
     7741 tgttcagaat gagtacgatg aactgttata atataacaat cccttacggg agaattccag
     7801 tggctttcct gcgttcggta aaatcccgtc gcggcatttt tgccgggggg atgacttata
     7861 aagggggcat ggactcaaca aaccgtagaa atctacagct ggctggcttg tggcgcgggt
     7921 ttcattgatg ttagcttatg ccaaaacctg gcatcttaaa gagtaaaagt atgttttgtg
     7981 tgatttatcg aagcagcaag cgtgaccaga cctatttata tgtcgaaaaa aaagacgatt
     8041 tttcgcgtgt tcctgaggaa ctgatgaaag gttttggtca gcctcagtta gcgatgattc
     8101 tgccgctgga tgggcggaag aaactggtca atgccgatat tgaaaaggtt aaacaggcat
     8161 taaccgagca aggttactat ttgcagttac cgccaccacc cgaagatttg ctgaagcaac
     8221 atctttccgt catggggcag aaaacagacg acactaacaa ataaccgata tccggcggtg
     8281 gcattatctt tgtcggcgcg ggttttcata tccacgataa ggtgagggga acgttatgta
     8341 tcaacatcac aactggcaag gtgcgctgct ggattatccg gtgagtaaag tagtctgtgt
     8401 tggcagtaac tatgccaaac atattaaaga gatgggcagc gcagtgcccg aagagccagt
     8461 gctgtttatt aaaccagaaa cggcactgtg cgatctgcgg cagccattgg cgatcccatc
     8521 cgatttcggt tcagttcatc atgaagtcga actggcggtg ttgattggcg cgacgctgcg
     8581 tcaggctacg gaagagcatg tccgcaaagc cattgccggt tacggcgtgg cgcttgatct
     8641 gacattgcgt gatgttcagg gaaaaatgaa gaaagccggg cagccgtggg aaaaggctaa
     8701 agcgtttgat aactcttgtc cgctttccgg gtttattccc gcggcggaat ttaccggcga
     8761 tccgcaaaat acaacgctgg gcctgagcgt aaacggcgaa caacgccagc aaggtacgac
     8821 tgcggacatg atccataaaa tcattccgct gatcgcttat atgagcaagt tttttaccct
     8881 caaggccggt gacgttgtgt tgacaggcac gcctgatggc gtcggcccgt tgcaaagcgg
     8941 tgatgagctg acagtcactt tcgatgggca ttctttgaca actcgcgttt tgtaatactt
     9001 tttgccgcct gaaagcggcg gcaaaacttg catcgctgtg ccagactggt tataaggtgc
     9061 gttttaacgt aatggcggaa cacctgatga gcgatgtacc tttctggcaa agtaaaaccc
     9121 tggacgaaat gagcgatgcg gaatgggagt cgttgtgtga tggttgcggt cagtgttgcc
     9181 tgcataaact gatggatgaa gacaccgacg aaatctactt cactaacgtc gcctgtcgcc
     9241 agctcaatat taaaacctgt caatgtcgga actacgaacg tcgttttgag tttgaacccg
     9301 actgcattaa attaacccgt gataatctgc caacattcga atggctgcca atgacctgcg
     9361 cttatcgttt gctggcagaa ggtaaagatt tacctgcgtg gcatccgcta cttactggtt
     9421 cgaaagcggc aatgcatggt gaacgtatct ctgtgcgtca tatcgcagtg aaagaatcag
     9481 aagtgattga ctggcaggat catatcttaa ataaacctga ctgggcgcag tgataaattt
     9541 aatagatgct gatgggggct ttcttaatat acgtctggat gctcgccatt tcctagccct
     9601 gtttttgaaa gcgctctgtc ataatgagag tt
//
LOCUS       DBANUU010000156         9462 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000156 DBANUU010000000
VERSION     DBANUU010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.158"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(84..2075)
                     /gene="tkt"
                     /locus_tag="ACIE68_003969"
     CDS             complement(84..2075)
                     /gene="tkt"
                     /locus_tag="ACIE68_003969"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFX5910212.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2353..3111
                     /gene="loiP"
                     /locus_tag="ACIE68_003970"
                     /gene_synonym="yggG"
     CDS             2353..3111
                     /gene="loiP"
                     /locus_tag="ACIE68_003970"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFX5910213.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKALSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(3317..4237)
                     /gene="speB"
                     /locus_tag="ACIE68_003971"
     CDS             complement(3317..4237)
                     /gene="speB"
                     /locus_tag="ACIE68_003971"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFX5910214.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4373..5104)
                     /locus_tag="ACIE68_003972"
     CDS             complement(4373..5104)
                     /locus_tag="ACIE68_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910215.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGMDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(5250..7226)
                     /gene="speA"
                     /locus_tag="ACIE68_003973"
     CDS             complement(5250..7226)
                     /gene="speA"
                     /locus_tag="ACIE68_003973"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFX5910216.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7235..7366)
                     /gene="yqgB"
                     /locus_tag="ACIE68_003974"
     CDS             complement(7235..7366)
                     /gene="yqgB"
                     /locus_tag="ACIE68_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFX5910217.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(7460..7603)
                     /gene="yqgG"
                     /locus_tag="ACIE68_003975"
     CDS             complement(7460..7603)
                     /gene="yqgG"
                     /locus_tag="ACIE68_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFX5910218.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(7714..7965)
                     /gene="yqgD"
                     /locus_tag="ACIE68_003976"
     CDS             complement(7714..7965)
                     /gene="yqgD"
                     /locus_tag="ACIE68_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFX5910219.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            8021..9175
                     /gene="metK"
                     /locus_tag="ACIE68_003977"
     CDS             8021..9175
                     /gene="metK"
                     /locus_tag="ACIE68_003977"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFX5910220.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPTARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2320 a         2526 c         2412 g         2204 t
ORIGIN      
        1 ctttatctat tgcttgtccg caaacggaca tatcaaggta ataaaaaagg tcgccgaagc
       61 gaccttttta cccgaaatgc taattacagc agttcttttg ctttcgcaac aacgttatca
      121 acagtgaagc cgaactcttc aaacagcagc tctgccggag cagattcacc gaaggtggtc
      181 ataccgacga tagcaccgtt caggccaaca tacttgtacc agtagtcagc aatacccgct
      241 tctacagcaa cgcgtgcagt aaccgctttc ggcagtacgg attcacggta agcagcatcc
      301 tgcttgtcaa atgcgtcggt agacggcatg gacaccacgc gcgctttcac gccttcggca
      361 gtcagttttt cgtaggcagc aacagccagt tcaacttctg aaccggtagc gatgaaaatc
      421 agttccggct gaccggcgca gtctttcagc acataaccac cgcgcgcgat gtttgccagt
      481 tgctcttcag ttcgttcctg ctgcgccagg ttctgacggg agaggatcag tgcggtcggg
      541 ccgtcctgac gctcaacacc gtatttccac gcgaccgcgg attcaacctg gtcacacgga
      601 cgccatgtag acatgttcgg ggttacgcgc agagaagcga cctgctcaac cggctggtga
      661 gtcgggccgt cttcgcccag accgatggag tcgtgggtgt aaaccatcac ctgacgctgt
      721 ttcatcagcg cagccatacg tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg
      781 gaggtgtacg gcaggaaacc accgtgcagg gagataccgt tagcaatcgc ggtcataccg
      841 aactcgcgaa caccgtagtg gatgtagtta cccgcagcat cttcgttgat tgctttagaa
      901 ccagaccaca gggtcaggtt agacggtgcc aggtcagcag aaccgccgag gaattccggc
      961 aacagcggac cgaaagcttc gatagcattc tgagacgctt tacggctggc gattttcgcc
     1021 gggttagcct gcagtttagc aatgaactct tttgctttcg cgtcgaagtc agacggcatt
     1081 tcgcctttca tacggcgggt aaattcagcg gcttcctgcg gataagcttt cgcgtaagca
     1141 gcgaatttct cgttccatgc ggattctttc gcctggcctg cttctttcgc atcccactga
     1201 gcatagattt cagacgggat ttcgaacggt gcatatttcc agcccagctg ttcgcgggtc
     1261 agggcaattt cagcgtcgcc cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc
     1321 ggggaaccga aaccgatgat ggttttgcac atcagcaggg acggtttgtc agtcactgcg
     1381 cgcgcttctt ctactgcgcg tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata
     1441 acgtgccagc cgtaagcttc gaaacgcatt gcggtgtcgt cggtgaacca gccttcaacg
     1501 tgaccatcaa tagagatacc gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc
     1561 gtacccgcca gagagcaaac ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg
     1621 aaggcgtagg tgtagtggtc gacaatgtcg tggcccggac ggttaaactg cgccgccagc
     1681 gttttttctg caatcgccat acctactgcg ttggcaatac cctgccccag cggaccggtg
     1741 gtggtttcca caccagcggt gtaacccact tccgggtggc ccggagtttt agagtgcagc
     1801 tgacggaagt ttttcagttc ttccatcggc agatcgtaac cggtgaggtg cagcaggctg
     1861 tagatcagca tggagccgtg gccgttggac agcacgaagc ggtcacggtc agcccaggac
     1921 ggattctgcg ggttgtgttt caggaaatca cgccacagga cttcggcaat gtcagccata
     1981 cccatagggg cacccgggtg accggatttg gctttctgta ctgcgtccat gctcagcgca
     2041 cgaatagcat tggcaagctc tttacgtgag gacattttga ctccagatcg gatgatgaag
     2101 ggcacgccct taacgacttg acgacagcgc gttttgggct acgccggaaa atttgccaac
     2161 aatttaccgc aagccgcgcg tcatgtacat ggaacatcct tttgccgctt cagaaatctc
     2221 tggatcatgc tcgcatgttg cgcaatctac tcgcccgtcc gctgcgcttt tccttatact
     2281 gagactgagc gtcgattcac ctgcaaacgg cgcattttta gaataatcct gaccttgtgc
     2341 ggaagagaaa acatgaaaat tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg
     2401 accggttgcc agaatatgga ctccaacgga ctgctttcat caggagcgga agcttttcag
     2461 gcttacagtt tgagtgatgc gcaggtgaaa gccctgagcg atcaggcatg tcaggagatg
     2521 gacagcaagg cgacgattgc gccagccaat agcgaatacg ctaaacgtct gacaactatt
     2581 gccaatgcgc taggcaacaa tatcaacggt cagccggtga attacaaagt gtatatggcg
     2641 aaggatgtga acgcctttgc aatggctaac ggctgtatcc gcgtctatag cgggctgatg
     2701 gatatgatga cggataacga agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg
     2761 ttaggccatg tgaagaaagg gatgcaggtg gcgcttggta caaatgccgt gcgagtagct
     2821 gcggcctctg cgggcgggat tgtcggcagt ttatctcaat cacaacttgg tgatctgggc
     2881 gagaaattag tcaattcgca attctcccag cgccaggaag cagaagccga tgattattct
     2941 tacgatcttc tacgccaacg cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa
     3001 ctggcgaaac tggaagaagg ccgccaaagc tcaatgtttg acgaccatcc ggcatccgcc
     3061 gaacgcgccc agcatattcg cgatcgtatg agcgcggatg gggttaagta aagcctggtg
     3121 gtgtcgaacg taggtcagat aaggcgttca cgccgcatct gacatgaatg gcacatttgt
     3181 taccttgtgc gcattgccgg atgcgatgct ggcgcatctt atccggccta cgggtgccga
     3241 acgtaggtcg gataaggcgt tcacgccgca tccgacatta atggcacgtt ttacccgtgc
     3301 gcatcgcatc tggtgcttac tcgccctttt tcgccgcctg aatatacagc atctccagcg
     3361 ccagcgtcgc cgcagccaga gcggtgattt ccgactgatc atatgccgga gccacttcca
     3421 ctacgtccat cccaacgatg ttgagatctt tcaggccgcg taccagttta atagcgcgat
     3481 cggaggtcag gccgccaatc actggcgtac cggtgcctgg tgcaaaagca ggatccaggc
     3541 agtcgatatc aaaggtcagg taaaccggca tatcacccac aatctgtttc acctgggcga
     3601 taacgtcatc cacgctacga tcgttcacct ggcaggcgtc cagtacggta aagccgttgt
     3661 ctttatcaaa ctcggtacga ataccaatct gtacggaatg attcgggtcg atcagacctt
     3721 ctttcggcgc ggtatagaac atggtgccgt ggtcaaattc acaaccgttc gcgtaggtat
     3781 cggtgtgggc gtcgaagtgt accagcgcca ttttgccgaa atgcttagca tgagcacgca
     3841 gcagcggcag cgtaacaaag tggtcaccac cgaaagagag catacgctta ccggcagcca
     3901 gcaacttctc ggcgtgtgcc tgcagttttt cgctcatctc acgggcatcg ccgaaggcat
     3961 ataccagatc accgcagtcc acgacgttca ggcgctcacg catgtcgaaa ttccacggga
     4021 agcggttgtg ttcccaggcc agattcgtcg aaacctgacg gatcgctgcc ggaccgtgac
     4081 gaccaccggc acggccagag gtggccatgt cgaacggcac gccggtgatc acccagtcgg
     4141 catcgctgtc atacggctgg aagttcatcg gcaggcgtaa aaaaccaaag gcgttggaaa
     4201 ccagtgagtt atcgtattga tgacctaagg tgctcatgga tggactccta ttacaaagtc
     4261 gatataaaaa aacccttccg cgtcgttagg cccgacgagg aagggttgga tttgcgaaac
     4321 acaagtttgt gctaattatc gccttaaatg ggcgaggcat caagcgaggt tgctatttcc
     4381 gacacggtgg cggcgcatag ccattgttat ctgccggaaa ggcttgtcca gagcacggcg
     4441 gcagcgcgtt ttgcgccggg ccttgtaatt caaaggcggc aaagccgatt accccggtat
     4501 tacgcacatc gccttgcgcc gaattggctg cgtaggaatc agcaacgttg gcgaactgaa
     4561 aagccgcttc ggtatgttta tctttgcgga atcctttaat cgctaacgaa tctccggcat
     4621 tcacaatata gccgttgtta ttcagcgaac cttgcttgcc gttcagcaca tccatgccat
     4681 caaccgtagc aacgatctcg tagttggtgt tacgactgta attacggaca tataattgat
     4741 aattcattcc gactctggca tggacaatat agcgtggttc attattggat gaatcataaa
     4801 cccgcgtaat aggtagagaa ttaaaattac catcgcgaat agcatattcc agttcgttaa
     4861 tacgaatgct ataaacgcgg tcgtagccgg aaggaacatt agtcgaataa ctaataacga
     4921 tcatctctgc cggatcgcgc caggctcgct cagcagatac gccctgaacc gttgagtgca
     4981 catccccacc ccaggcagtg ccgacattgc ccgatgcact gctattatac tgcgcattac
     5041 tggaacaacc ggccagcagc acgattaatg ccaccaacgc gctacgtact ttccattttt
     5101 tcataaccgt tccctctgca tttaattaat gcagcgattg tagggagatt atttaataag
     5161 aaaagcagcg agattatttt taagaatata taaaaagaaa gcgggccgat ttacgtaaat
     5221 aaatcgaccc gccagcggaa taatcttaat tattcatctt caagataggt ataaccgtac
     5281 aaacctgcct cgaactcttc aaggaactgt tgttgcagtt cagcatcaag atcggttttc
     5341 ttcacttgat cgcggaactg ggttaacagc gttttcggat cgagttgtac atattgcagc
     5401 atgtccgcca cggtatcgcc ttcgtcagac agttctactt ctacgctacc gtcagggaac
     5461 acgaacacgt caaccgcttc ggtatcaccg aacaggttgt gcatattgcc gaggatctcc
     5521 tgatatgcgc cgaccataaa gaaaccgagc atcggcggat tctctggatc gtactccggc
     5581 attggcattg tcgtggcaat accatcacca tcaatatagt ggtcgatagc accgtcagag
     5641 tcacaggtaa tatccagcag cacagcgcga cgttccggca cttgatccaa cccttccagc
     5701 ggcagaaccg ggaacaactg gtcgatcccc cacgcatccg gcatcgactg gaacagcgag
     5761 aagttgacgt acattttgtc cgccatacgt tcctgcagct cgtcgataat cggacgatga
     5821 gcacggtttt gcggatccag ttgcttctgc acttcatggc acatgctcag atagagttgt
     5881 tctgcccatg cacgttcttg caggctaaag gtgccggaag agtagccgat atgaatgtcg
     5941 tgcagatcca tctgactgtc gtgtaaccat tcacgcagag aacggcgagt tcccggttcg
     6001 tgcatctcct gccaggtttc ccacatgctt tgcagcgcgc gcggcgcatc ttctgcaggc
     6061 gcggtcggca ccgtgtattc gttacgttcc acgccgatga tattagatac cagcacggtg
     6121 tgatgcgcag tcaccgcacg acccgattcg gtgattaccg tcggatgcgg cagaccgttt
     6181 tcttcacacg catcaccaat cgcccagatg atgttgttgg catattcatt gaggccgtag
     6241 ttcaccgaac agtcggactg cgaacgggtg ccttcatagt ccacgcccag accgccgccg
     6301 acgtcgaagc actgaatatt gacgcccagc ttgtgcagtt ccacatagaa acgcgcggat
     6361 tcacgaacgc ctgtcgcgat atcgcgaata ttcgccatct gcgaaccgag gtggaagtgc
     6421 agcagttgca ggctgtcgag acgcccggct tcacgcaggg tttcaacaag ttgcagtacc
     6481 tgagtcgcag ccaggccgaa cttcgatttt tccccgccgg aggactgcca tttacccgag
     6541 ccctgcgaag ccagacgtgc acgcacgccc agacgaggaa cgacattcag acgttctgct
     6601 tcatccagca caatggcgat ttctgacatc ttctcaatga ccagatagac cttgtgcccc
     6661 atcttctcgc caattaatgc caggcggata tattcgcggt ctttataacc gttgcagacg
     6721 atgacgctac gggtcatgcc agcatgcgcc agcaccgcca tcaactctgc tttcgagcct
     6781 gcttccagcc ccaacggttc gccagaatga atcagggact caatcacgcg gcggtgctgg
     6841 ttaactttga tcggataaac aaggaagtaa tcgccgttat agccgtagga ttcccgcgca
     6901 cgtttgaacg cggcgttaat ggaacgcaaa cggtgctgca ggatctgtgg gaaacagaac
     6961 agtgcaggca gacgctgacc ctgtgcttca cgagttttca ctaactgcgc gagatcgacg
     7021 cgagcttccg ggacgtccgg gtccgggcac acgctgatgt ggcccagctc gttaacgtca
     7081 tagtagttat tgccccacca ggcaatattg taagtacgca gcatcttgct ggcttcctgg
     7141 gagctcattg caacctcctg catggagcgt agtacaccgt gttcgcccgc tgacgaaggc
     7201 aaacccatag acatgtcgtc agacatagcg aacctcaaat tattttatta agtgtaaaac
     7261 agttaacgac tatcgcagcc tggaactgcg ataacaaccc ataagcacat ggattttcca
     7321 gcagtgaatg ctgacgctcc aactgcgcaa ccggtttctt tttcataaca ttattaagca
     7381 cataaccgaa cgtaagtgtg acagttcggc gaaaccacga gaaaactctt gtttttacaa
     7441 gagcgccctt gttcagtcct cagtaactgt aaccagctct tgaatcctga gaagcgccga
     7501 gatgggtata acatcggcag gtatgcaaag cagagatgca gagtgcgggg aacgaatctt
     7561 caccagaacg gtgagacagg ttaagcagca gacaacggtt cattatttcg tatcacctcc
     7621 acggccgcct gttaagacga acccacaagc caaaactctg atttcaaccc ggctggaagt
     7681 ggcaacacga aagaaacgtc gtgtgctttt tatttaagcc gcgcgccgcg ttttataccc
     7741 cacaatggca gaaaattgca aaagataaat acgcagaatg ccggcattgt caggaaaaat
     7801 ttccagccac gtttttaaca gaatgagaca cgattcaaaa aaaagtggaa atcgggtgaa
     7861 gaattgacct aaaatagcca tccagatgtt aatccatcca taccgattaa cactcagact
     7921 gccagtgttt ttaacttgca gagtcgtggt aggatccgct accacagaaa atccacacaa
     7981 cagtttgagc taaccaaatt ctctttaggt gatattaaat atggcaaaac acctttttac
     8041 gtccgagtcc gtctctgaag ggcatcctga caaaattgct gaccaaatct ctgatgccgt
     8101 tttagacgcg atcctcgaac aggatccgac agcacgcgtt gcttgcgaaa cctacgtaaa
     8161 aaccggcatg gttttagttg gcggcgaaat caccaccagc gcctgggtag acatcgaaga
     8221 gatcacccgt aacaccgttc gcgaaattgg ctatgtgcat tccgacatgg gctttgacgc
     8281 taactcctgt gcggttctga gcgctatcgg caaacagtct cctgacatca accagggcgt
     8341 tgaccgtgcc gatccgctgg aacagggcgc gggtgaccag ggtctgatgt ttggctacgc
     8401 aactaatgaa accgacgtgc tgatgccagc acctatcacc tatgcacacc gtctggtaca
     8461 gcgtcaggct gaagtgcgta aaaacggcac tctgccgtgg ctgcgcccgg acgcgaaaag
     8521 ccaggtgact ttccagtatg acgacggcaa aatcgttggt atcgatgctg tcgtgctttc
     8581 cactcagcac tctgaagaga tcgaccagaa atcgctgcaa gaagcggtaa tggaagagat
     8641 catcaagccg attctgcccg ctgaatggct gacttctgcc accaaattct tcatcaaccc
     8701 gaccggtcgt tttgttatcg gtggcccgat gggtgactgc ggtctgactg gtcgtaaaat
     8761 tatcgttgat acctacggcg gcatggcacg tcacggtggc ggagcattct ctggtaaaga
     8821 tccatcaaaa gtggaccgtt ccgctgcgta cgcagcacgt tatgttgcaa aaaacatcgt
     8881 tgctgctggc ctggccgatc gttgtgaaat tcaggtttcc tacgcaatcg gcgtggctga
     8941 accgacctcc atcatggtag aaactttcgg taccgagaaa gtgccttctg aacaactgac
     9001 tctgctggta cgtgagttct tcgacctgcg cccatacggt ctgattcaga tgctggatct
     9061 gctgcacccg atctacaaag aaaccgcagc atacggtcac tttggtcgtg aacatttccc
     9121 gtgggaaaaa accgacaaag cgcagctgct gcgcgatgct gccggtctga agtaatcttt
     9181 cttcacctgc tttcaaaggc cagcctcgcg ctggcctttt tcttttacct caccgcggtg
     9241 gataggcatt cacgccgcat ccggcaaaaa gctgcccgca caataacatc attcttcctc
     9301 aacacgtttt atcgcaaatt atcatcacta ctgaaaccga ttacaccaat aacaccagcc
     9361 aaatatttgt aatattttca tattattatt cagttttcac agttgttaca tttcttttca
     9421 gtaaagtctt aattgcagat aacagcgttt aatctatgat ga
//
LOCUS       DBANUU010000157         9447 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000157 DBANUU010000000
VERSION     DBANUU010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.159"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(77..1606)
                     /gene="yeeO"
                     /locus_tag="ACIE68_003978"
     CDS             complement(77..1606)
                     /gene="yeeO"
                     /locus_tag="ACIE68_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFX5910221.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKSQLCRNKRQNADYFSKRFQ
                     I"
     gene            1767..1842
                     /locus_tag="ACIE68_003979"
     tRNA            1767..1842
                     /locus_tag="ACIE68_003979"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1800..1802,aa:Asn,seq:gtt)
     gene            complement(1880..2830)
                     /gene="cbl"
                     /locus_tag="ACIE68_003980"
     CDS             complement(1880..2830)
                     /gene="cbl"
                     /locus_tag="ACIE68_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFX5910222.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPHDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2932..3849)
                     /gene="nac"
                     /locus_tag="ACIE68_003981"
     CDS             complement(2932..3849)
                     /gene="nac"
                     /locus_tag="ACIE68_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFX5910223.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVMSSPVMEKRQWQLVS"
     gene            4175..4250
                     /locus_tag="ACIE68_003982"
     tRNA            4175..4250
                     /locus_tag="ACIE68_003982"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4208..4210,aa:Asn,seq:gtt)
     gene            complement(4306..5241)
                     /gene="ldtA"
                     /locus_tag="ACIE68_003983"
     CDS             complement(4306..5241)
                     /gene="ldtA"
                     /locus_tag="ACIE68_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFX5910224.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5303..6382)
                     /gene="cobT"
                     /locus_tag="ACIE68_003984"
     CDS             complement(5303..6382)
                     /gene="cobT"
                     /locus_tag="ACIE68_003984"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFX5910225.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6394..7137)
                     /gene="cobS"
                     /locus_tag="ACIE68_003985"
     CDS             complement(6394..7137)
                     /gene="cobS"
                     /locus_tag="ACIE68_003985"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFX5910226.1"
                     /translation="MSKLFWAMLSFITRLPVLRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7134..7679)
                     /gene="cobU"
                     /locus_tag="ACIE68_003986"
     CDS             complement(7134..7679)
                     /gene="cobU"
                     /locus_tag="ACIE68_003986"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFX5910227.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIYADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(7813..8004)
                     /locus_tag="ACIE68_003987"
                     /pseudo
     CDS             complement(7813..8004)
                     /locus_tag="ACIE68_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            complement(7983..8171)
                     /locus_tag="ACIE68_003988"
                     /pseudo
     CDS             complement(7983..8171)
                     /locus_tag="ACIE68_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(9168..9447)
                     /locus_tag="ACIE68_003989"
                     /pseudo
     CDS             complement(9168..9447)
                     /locus_tag="ACIE68_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2594 a         2530 c         2017 g         2306 t
ORIGIN      
        1 ccgactgaac tacagaggaa tcgtgagaac gaggcgaata ttagcgatgc ccacccacaa
       61 tgtcaaagcc tgttttttaa atttgaaatc gtttgctgaa ataatctgca ttttgtcgtt
      121 tattccgaca caactggctt ttttcacact tttgcggctc gggtcgaggg tatttccata
      181 gccaacgtcc agtaaccatt cgccagtaaa acagcacggc ccgcacagcc cagtcggcaa
      241 acattcccat ccagacacca accacacccc agccaagcat gattcccagc acataaccga
      301 ctacaacccg acaaccccac atgctcaaca tcgaaaccca catggcgtaa cgggcatcac
      361 gagcaccttt aaatccagcg ggtagcaccc atgaggcgga ccaaataggc ataaataaag
      421 catttagcca aatcagaatc acaacgacat gtttaacctg tggatcctgg gtgtaaaacg
      481 atgccataac cccggcaaag ggagccgtta gccaggcgat ggccgttaat ccaagagtgg
      541 aaagccagaa cacatgccgc aactgaatct ctgcttgcgc tatctgccct acccccaacc
      601 ttcggcctgt aatgatcgta gaagcagagc cgagcgcact tccgggtaag ttgataagag
      661 ccgcaattga aaacgcgata aaatttccgg caataacact ggtccccatc ccggcaacga
      721 acatttgggt taataaccga ccactggtaa ataacactga ttcgacactc gcgggaatac
      781 caatccccat gacttcccag ataatgctaa aattcagcgg tttaaaatag ctctttaacg
      841 aaatccttag cgcaggatta aaaccaatcg ccagcaccca caaaattgca actgcgccaa
      901 tataacgaga aatggttaaa cccagccctg ccccgacaaa tcccagtccc ggccaggaga
      961 aaaggccgta aatcaatatg ccgctaataa taatattaag aatattcagg ctaccgttaa
     1021 tcaatagcgg tattttcgta ttccctgcac cacgaagtgc cccgctacca ataagagtga
     1081 tggcagctgc tggataactg agtaccgtca gctccagata agtcaacgcc agtgctttaa
     1141 cttctgtcgt ggcatcaccc gcgacgaaat caataatttg ctcgccaaaa tgatgaataa
     1201 gcgttgccaa cagtacggca aacaacgtca tgatcaccaa tgactgccgc gtcgccaccc
     1261 tcgctcgtcg tcgatcccgc ttaccgagac taaatgccac aacgacagta gtaccaagat
     1321 cgatagcagc aaaaaaagcc ataatgacca tattgaagct gtccgccaat cccacgccgg
     1381 ccatcgcatc ttttcccagc cagctgacca gaaaagtgct cagaaccccc atcaacagga
     1441 cacaggcatt ctccatgaag ataggaacag caagcggggt tatctcgcgc cagaacaaca
     1501 ctttgtagct cttgcgttta gcgtgccagc gagtgccgtg aacaacctgg cgtaaagcag
     1561 aggagatatt caaagccgac cttaattgca gaaagtgaaa ccacatttca aataatgagg
     1621 gagaatcagc aaagctgcaa agattttcgc caacaaattg tctacaaatg caacaaactg
     1681 ttgatagaaa cggcaaacag ttggggaatt taaaaatcgg gtttgacaaa agatttttcg
     1741 ccgttaagat gtgcctcaac agcgattcct ctgtagttca gtcggtagaa cggcggactg
     1801 ttaatccgta tgtcactggt tcgagtccag tcagaggagc caaatttctg aaaagcccgc
     1861 ttttatagcg ggccttttgc tatatctgat aatcaatttc ctcttcactg ttttccatca
     1921 cctggcgctt gatatcctca actgacagtc ctgcattaca aagttccaga aagcgccaga
     1981 catagttacg ctgaagttgt cctcgcttga gtcccaacca gacagtatta gcatcgaaaa
     2041 gatgccgcgt atccaggcgg attaaattct tttcctcttg ttcaccgctg gattgttcgg
     2101 caactaatcc gatcccaagc ccaagagcaa cataggtttt aatgacatca gaatcctgcg
     2161 cacttaatac aatatctgcc agcaaacctt tgcgggcaaa tgcgtcatca atacgtgagc
     2221 gccccgtaat cccctgtcgg taagtgatta acggccactt cgctattgat tccagcgtca
     2281 atggtgtaat ttgcgtcaag ggatgatcgt gtggaacaag caaactatgg tgccaacaaa
     2341 accacgggaa ggcgacgagc tgcgggtcat tactcaaacg ctcgctggcg ataccaatat
     2401 cagcttcgcc attttgcaac aacgtcgcaa tttcctgtgg cgtaccctgg attagctcca
     2461 gccgaacctc cgggaaaagt tcgcgaaaag ctttaatgac ctcaggcaag ctataacgtg
     2521 cctgagtatg cgttgttgca atagtgagaa cgccagacgt atcgttggta aacaggtctg
     2581 caagccgacg aacattactg gcttcattca gaatacgttc tgcaatgacc agtaatgctt
     2641 tgcccggttc agtcatgccc agcagtcgct tacctcgtcg aacaaatatt tcgatgccaa
     2701 gttcatcctc cagttcccga atatgacggc tgacgccgga ctgtgaggta aaaagcatat
     2761 tcgcaacctc tgtcaggttg taatcctgac gtgcagcctc gcggattatc tttagttgtt
     2821 ggaaattcac ggtaaactcc gggcagttca gattttccgt tattgttaaa gtctaatgcc
     2881 cggcataaca aataataaaa acccgcatct tattccatcc cgatataacg cttagctcac
     2941 caattgccac tgcctttttt ccatcactgg agaactcatc actgacatta acaactcttt
     3001 cactgcctgt gcctgtggtg ataagttcgc tctggcgggt aaatttaatg acaaagagag
     3061 actcatggaa ggtgtggtaa tgcgtgacat ccacccgttt actgcgccac ataacgaacg
     3121 cgcggccgat tcgggtaata ctgcaacgcc catgccgctg gcaatcgctg cggtaagcgt
     3181 ggcaatagac tcaatttcac caatgacttt tgccgtgagt cgccgtaggg aaaaagcctc
     3241 atcaacacga agtctaacag cactgtaatc actgggaaga aagaggttca tttgcgcaat
     3301 agcattaaca tcaacgcttt gccccggaca atcttgagtc cctaccagga aaagatcttc
     3361 tttcagtaaa gcctgactgg atacaccagc cacaggggaa tgctcataaa tcaccgccat
     3421 atcgagttgg tgatttatca gtttttcgtt aagcactgca ccactatttt catgaagata
     3481 gataacgacc tccgggaatt cagcgcgaac cgcctgtaat aagggcatgg tgatggatga
     3541 cgcagcggtt cctggcgcaa agccaatcga gacttgcccc gataatgact gaccaacgtt
     3601 atgcaccgcc agttgggcct gctcacactg acgtaaaatg gcccgcgcat gggtatagag
     3661 aatttttccg gcgtctgttg gcgtaacgcc ccgttttgtg cgaatcaaaa gttgttgatt
     3721 taactcacct tccagtgtgg caacctgctg gctgagcgct ggttgtgcga tatgcaatac
     3781 ttcagcagcc tgagtcaggc taccaatatc tacaattttt acgaagtatt tcaggcgtct
     3841 gaagttcatg ttgcctccgg tttttgagaa tcggcccaag tgccgccatt acttacaacc
     3901 agactgcaag atgcttgcca gttttatttt ggcgttgatg tacaagctaa ccaactgtca
     3961 aataagagat tatgatagat tcgtcattgg ctcttttcac cagcagtcgc gttcccctgc
     4021 cctataaaag gagggtatgc accacgatgg ttcattacca ataagattga aatctcatca
     4081 ctttgttgaa attgacagca aacaaacaaa aaaatgcatt tcaccctttg acatcaccat
     4141 gcactgccat taatatgcgc cccgttcaca cgattcctct gtagttcagt cggtagaacg
     4201 gcggactgtt aatccgtatg tcactggttc gagtccagtc agaggagcca aattcaaaaa
     4261 agcctgcttt ctagcaggct ttttgctttc taattaccaa cgctcttaaa acatctgtct
     4321 tgaaccagaa ctaatttgca caggcattcc cgatcgacgt tgcaacgcag catttgcgcg
     4381 attcacatca acttcttgcc cgttgataaa cgcccgcaaa gatggggtta ccggcaatgg
     4441 cacttttcgg tcagactcat attctgcacg attgcgcgac agcggctcat gaacttccag
     4501 ccagttcgag ccatctggtt cagtggtgta ttttactggc tggtcgataa tttgcacacg
     4561 cgttccaaca ggaacattat caaacagata tttgatatcg tcattgcgca gacgaataca
     4621 gccctgactt acccgaagtc caataccaaa attggcattg gtaccgtgga tggcatacaa
     4681 cctgccaata taaatcgcgt acagccccat gggattatcg ggccccgcag gaacaaatgc
     4741 gggcaaactc tcccctcgtt tcgcatattc gcgccgagtg ttcggcgttg gcgtccaggt
     4801 tggagcttct tgtttacgtt caacggtagt cacccagtta cgcggggttt ctcgcccagc
     4861 ctggccgata ccaataggaa agacttccac agtattactg tctggcgggt agtaataaag
     4921 acgcatctca gcgacgttaa ccacaatccc tttacgaaca gtggcgggca aaatcagttg
     4981 ctgcggaatg gtgagttgcg agccagactt cggcaagaaa acatcagcgc ccggattcgc
     5041 ttccagcatg ttacttaacc cttgcccgta ttgtgcggca aaagtctcca gcggctgggt
     5101 attgtgatca ggaacagtta cagtaagcga ctgccccacc aaacggctac cctctggagg
     5161 taatggataa gttaccgcca ggctagtatg gctggcaaaa agcagagcaa atgagcaaag
     5221 aatatttaca cgacgcatca tgtccctttc ctatgtcgcg aaagctatcc gttaagtata
     5281 gcttttatca gacttttcgt ttttaactgt tcaaatcaga agtcgtattc cccggtagaa
     5341 caatattact ggcagcaagt tcgcccatgt tgttatatat cgcacaggca gcttcgatga
     5401 tgggcatcgc cagagctgcg ccactcccct cacctaaacg catctccata ttgagataag
     5461 gctccagccc caaatgcgac agcgctatac gcgccccttt ttctgccgac aggtgagaag
     5521 gaatgagata cggtttgatt gcaggagaca tctggcaggc tgcgagcgca gcagcataag
     5581 aaagaaatcc atccagcaac acgggtaaac cgcaggaagc agcgcctaac atcacgccag
     5641 ccattccgac caaatcaaat ccccccactt ttgccagaac atcaacgcca tcctgcggat
     5701 ttggttgatt caacgtaatc gcccggcgca caacatcaat tttattagcc agtttatctg
     5761 tcggcaggtt tgcgccaatc ccaaccactt cttcaggatc ccggccagtg attgtgctga
     5821 ctattgccgc tgccggtgtc gtgtttgcca tccccagttc acctacacca aacagcgtga
     5881 caccgttttt tgccagctcc cgcgtataac atatgacgtc caaaagcaac ttttcagcct
     5941 gacggcgact cattgccgga gctgaagcaa tattgccgct acctcgtgcg acacgcatgt
     6001 tgataagccc ggggataggc tcagcactat caataccaac atcaactacg tggacgttag
     6061 cgcccgcttg tgctgccagc acacacacgc cggttgttcc gcgggtcata ttttcagcct
     6121 gaatggctgt cacttctttt ggggaaatag cgaccccttc ctcccagacg ccgtgatcgg
     6181 cacacataac cagtaccgct tttttgccca catgcggtat gccattcaac cccggcattc
     6241 ccgccagttg tatggcaagc gcctccagcc ttcccaggct accaacaggt ttgagtaacc
     6301 cgtcaatatg ccgttgtgca cgcgacatag cggcaggatc gatagcaggg atcgtattca
     6361 gtaaatcggc aagtgtttgc atgtcacgtc tctttatagc agtgccagca ggaagaccag
     6421 ttcaccaagc tctatagcag ctcccagcgt atcgccggtt tgcccgccca gcgtacgttt
     6481 gagaagctga ccgaggatga aaatcgccac catcgtcacc accatagcag ccacaccatg
     6541 catacccggc aataatacgg cagcaaaaat agcggctaag ccgagtgtga cgcaggtttg
     6601 tcgcccatca attttgccaa tgaacacatt gccaagccct tcttcccggg cataacgatg
     6661 acgatacatc aataatgcgg cagtcccgcg gctgaccgca catgctgccg ccagcgatgc
     6721 aaggatcggt tcgccacgca gagccaactc gcttaatacc agaatctttg ccagtacaac
     6781 aaaaatcaat gccagaccgc catgggtgcc taaacggcta tcacgcatga tttccaacat
     6841 gcgatcgcgg ctacgtgcag aaaatacgcc atcacaggta tcggcgaggc cgtctaggtg
     6901 gaatcccccg gtcatcagcg ccagcacaag tacgctaaac agtgccgcca gtggtacgcc
     6961 acaccatgcc tgcagcacca tgaagaccag cccgctaatc gcgccaagca ataatccaat
     7021 taaaggaaaa gtaataatac cgcgagaata atgctcgaaa tccagtccct gggaccagcg
     7081 acgcagtaca ggcagacgcg taataaatga gagcatcgcc caaaataatt tactcatttg
     7141 atttttactc caatacccga aaccaccagc catacttcat ttgccgcagc ggccagttgc
     7201 tgatttaccc gcccggcaat atcacgaaaa tgtcgtgcca gacgactctc cggcacaatc
     7261 cccattccca cttcgttagt cactaataca acctttgcgg ggcaacgttg gcaggcagca
     7321 atcaacgact gaatctcagc attaatcgcc tgttccatcg cctgataatc ccattcatca
     7381 gggtctttat cgccgccata atcaaacaac agattagtca ccattgtggt aacgcattcc
     7441 agcaacacag cttcatgcgg gttaatgtct gcataaatta attcatcaag atgttgccaa
     7501 cgctccaccg tgcgccagtg cgccggacgg ccttgccgat gatgttctat ccgtgcagcc
     7561 atctcgtcat caaggatttg cgaggtggcg atatacagaa cctgtgaaga gtccccaata
     7621 agcgcctctg cgtggcgact cttcccgctc cgtgcgcccc ccgtcaccaa aatcatcata
     7681 ccgactcctg attcaattgt atgatggtat tcattgaaca gaacaatgga tcccccacca
     7741 ttgctgaccg caaaacattt acacgatcaa tactgcgcgt aatataatta aaaatccagc
     7801 attctcaatc cattacgaaa gaagatggca ttgtggaata gccagacttc ctcgtgttaa
     7861 cgtccgacaa aatgcgtcat tgacagcggt ttctgtggat tgacacattt cattcagata
     7921 atgaattaat gctactgccg gaccaaccag caaacgggca ttgtgttctg aaatccaggc
     7981 tattgattca acgtcagcga cagatctgcg ctgtaaaaga acttgtaaaa caacgcaata
     8041 gaattatccg gaatggtggc gactatgcac tagaacacca caatttcgct ctctcggtaa
     8101 ggcactgtca cagcattcaa cagaatgtga cttgccagat ttattagcgc caccagatgt
     8161 ttaaccgggt aaccacgacc cagtcgggga tatattccat cttctcgctg acatcatcaa
     8221 gaataaaaag gtttatcaca ctcagagcaa cgttcttcag caggcgggat aattcttcaa
     8281 catttaaaag atgtgttatt aaaggcatac aaactggacg tattgtattt tcttttggtg
     8341 gttgacctaa ggtagcagtt tatcctgatg cgctgagatt tctgtaatat ctacgtcaac
     8401 attctcctcg aatagaaaat gcagcctcgg caagtgattc acatttttta acaaaacgtt
     8461 gttagcgtgc cattttcaga agctctccaa gaaagcgaat tcttttctca aattctgcat
     8521 tctcagacac agctttctac tccatggcaa tggcccaaat acgaagttgc tcaggatcat
     8581 tagtggtgct gagaagttaa ataaccatgc ggagtaccac tcgtcatcac gtatttatat
     8641 atcatgaagc tattgttgtg ttttgtacat cagtagatgt attctgatat actccttttg
     8701 ctagacataa cctttcacct gcttgcaaag cttctgtgtt ctgacattgc caaattgttg
     8761 caattctgta tccagccttc tttcagtcat agcttcgggc cgcgataaga ctcactgatc
     8821 tgaccctgat tcctcttgca gactttatag accaattaaa atgcagtttc tgcaggtcaa
     8881 cgtctgacca tcattgtcat cactctggcc attagagtaa ccttctgcat tcatcctttt
     8941 gtaaaaagtt tatgtcagta tcagcaatta accggacctg atactgatat gagtcttacc
     9001 gcatatacgg tcaatttcag caattaatta cattatccac gccaaagtgt ttgtcatcgc
     9061 aatgatggta ccttctttca gacaccattt tttcaactcc gttttccacg gaccgcactc
     9121 ttatgtcaag agtgcggtga tcctacccac gtaatatgga cacaggccta agcgaggttc
     9181 tggttttcaa attgttccgg actgaggccg ccacaccaac tgtgccgccg ccaccgattg
     9241 taatcacatt cgatataatt aaacaccgtt gcccgcatta tttcccggct gataaagtgt
     9301 tctccatgga tacattccac tttcagcgaa tgaaagaagc tttccacgca gattatgccg
     9361 cttcagttgc gcctgataat ctgctgaaca gtactggcct ccacggtccg tgtgaacgat
     9421 aacgttccgg ggcctcttac gccgcca
//
LOCUS       DBANUU010000158         9225 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000158 DBANUU010000000
VERSION     DBANUU010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.160"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..115)
                     /locus_tag="ACIE68_003990"
     CDS             complement(<1..115)
                     /locus_tag="ACIE68_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFX5910228.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKT"
     gene            complement(202..270)
                     /gene="ykiE"
                     /locus_tag="ACIE68_003991"
     CDS             complement(202..270)
                     /gene="ykiE"
                     /locus_tag="ACIE68_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFX5910229.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            407..928
                     /gene="fecI"
                     /locus_tag="ACIE68_003992"
     CDS             407..928
                     /gene="fecI"
                     /locus_tag="ACIE68_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFX5910230.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            925..1878
                     /gene="fecR"
                     /locus_tag="ACIE68_003993"
     CDS             925..1878
                     /gene="fecR"
                     /locus_tag="ACIE68_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFX5910231.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1965..4289
                     /gene="fecA"
                     /locus_tag="ACIE68_003994"
     CDS             1965..4289
                     /gene="fecA"
                     /locus_tag="ACIE68_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFX5910232.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLTSRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            4334..5236
                     /gene="fecB"
                     /locus_tag="ACIE68_003995"
     CDS             4334..5236
                     /gene="fecB"
                     /locus_tag="ACIE68_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFX5910233.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5233..6231
                     /gene="fecC"
                     /locus_tag="ACIE68_003996"
     CDS             5233..6231
                     /gene="fecC"
                     /locus_tag="ACIE68_003996"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFX5910234.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6228..7184
                     /gene="fecD"
                     /locus_tag="ACIE68_003997"
     CDS             6228..7184
                     /gene="fecD"
                     /locus_tag="ACIE68_003997"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFX5910235.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7185..7952
                     /gene="fecE"
                     /locus_tag="ACIE68_003998"
     CDS             7185..7952
                     /gene="fecE"
                     /locus_tag="ACIE68_003998"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFX5910236.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(8509..8766)
                     /locus_tag="ACIE68_003999"
     CDS             complement(8509..8766)
                     /locus_tag="ACIE68_003999"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910237.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(8849..9225)
                     /locus_tag="ACIE68_004000"
                     /pseudo
     CDS             complement(8849..9225)
                     /locus_tag="ACIE68_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014615349.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT         2013 a         2630 c         2545 g         2037 t
ORIGIN      
        1 atgttttacg gcaatgagag cagagatagc gctgatgtcc ggcagtgctt ttgccgttac
       61 gcaccacgcc ttcagtagcg gagcaggaag gacatctgat ggaaatggaa gccacgcaag
      121 caccttaaaa tcaccatcat acactaaatc agtaagttgg cagcattacc gaaatcccca
      181 tcctttaggg cagtgaggat gtcaataata gcagaccaca tcatgagtca ttctgaccga
      241 cacatgccag aagcagaggc tccatcccat tttataccta ccttataaca cttagaaaaa
      301 caacatgtta aaaatgtcta ttggaaacaa ttttatttcc aattgtaatg ataaccattc
      361 tcatattaat atgactacgt gataattaac ttttgatgca ctccgcatgt ctgaccgcgc
      421 cactaccaca gcttccttaa cgttcgagtc gctttatggc acacatcacg gctggttgaa
      481 aagctggctg acgcgcaaac tccagtctgc ttttgatgca gatgacattg cccaggacac
      541 ttttttgcgg gtaatggtca gcgaaacgct ctcgacgatc cgcgatcctc gctccttcct
      601 ctgcactatc gccaaacgcg tgatggtgga cctgtttcgc cgaaacgcgc tggaaaaagc
      661 gtatctggag atgctggcgc ttatgccgga ggggggagcg ccttcacctg aggaacgcga
      721 aagccaactc gagaccctac aactcctcga cagcatgctg gacgggctaa acggcaaaac
      781 acgtgaagcg tttctgcttt cgcaactgga tggtctgaca tacagcgaga ttgcgcacaa
      841 actcggtgtt tccatcagct ccgtgaaaaa atacgtggcg aaagccgtcg agcactgcct
      901 gctgttccgt ctggagtatg ggttatgaat cctttgttaa ccgattcccg ccgtcaggcg
      961 ctgcgttcag cttcccactg gtatgccgtg ctaagcggcg agcgcgtcag cccacaacag
     1021 gaagcgcgct ggcaacagtg gtatgaacag gatcaggata accagtgggc ctggcagcag
     1081 gttgaaaacc tgcgcaacca gcttggcggt gtgcctggcg acgttgccag ccgggcgttg
     1141 cacgataccc gcctcacccg ccgtcacgtg atgaaaggat tactgctgtt gctcggcgct
     1201 ggcggaggct ggcagctctg gcagtcggaa accggcgaag gtctgcgggc agattaccgc
     1261 accgccaaag gcacagtcag ccgtcagcaa ctggaagatg gctccctgct cacgctgaat
     1321 acccaaagcg cggcggatgt gcgttttgat gcgcatcagc gcaccgtccg gctctggtac
     1381 ggtgaaatcg ccattactac cgcgaaagat gccctgcaac gccccttccg tgtcctgacc
     1441 cgtcagggcc agctcactgc tttagggaca gaatttaccg tccgccagca ggataatttc
     1501 acgcagcttg acgttcagca gcacgctgtg gaagtgcttc tcgccagtgc ccccgcgcaa
     1561 aaacgcatcg tgaacgctgg tgaaagcctg cagttcagcg cctctgagtt tggcgcagtg
     1621 aaaccgctgg atgacgagag tacaagctgg acgaaggaca tcctgagctt cagcgataaa
     1681 ccactgggtg aggtgatagc cacgctaacc cgttaccgca acggcgtgct gcgctgcgat
     1741 cccgccgttg ccgggctgcg cctgagcggg acgttcccgc tgaaaaatac cgatgcgatc
     1801 ctgaacgtta tcgcgcaaac gcttcccgtt aaaattcagt ctattacgcg gtactggata
     1861 aacatttcac cactgtaagg aaaataattc ttatttcgat tgtccttttt acccttctcg
     1921 ttcgactcat agctgaacac aacaaaaatg atgatgggga aggtatgacg ccgttacgcg
     1981 tttttcgtaa aacaacacct ttggttaacg ccattcgcct gagcctgctg ccgctggccg
     2041 gtctctcgtt ttccgctttt gctgcacagg ttaatatcgc accgggatcg ctcgataaag
     2101 cgctcaatca gtatgccgca cacagcggat ttaccctctc ggttgacgcc agcctgacgc
     2161 gcggcaagca gagcaacggc ctgcacggcg attacgacgt cgagagcggc ctgcaacaac
     2221 tgctggacgg cagcggactg caggtaaaac cgctgggaaa taatagctgg acgctggagc
     2281 ccgcgcccgc accaaaagaa gatgccctga ccgtggtcgg cgactggctg ggtgatgcgc
     2341 gtgaaaacga cgtatttgaa catgctggcg cgcgtgacgt gatccgccgt gaggatttcg
     2401 ccaaaaccgg cgcaaccacc atgcgtgagg tacttaaccg catccctggc gtcagcgcgc
     2461 cggaaaacaa cggcaccggc agccacgacc tggcgatgaa ctttggcatc cggggcctga
     2521 acccgcgcct caccagccgc tcgaccgtcc tgatggacgg catccccgtc cccttcgccc
     2581 cttacggtca gccgcagctt tcactggctc ccgtttcgct cggcaacatg gatgccattg
     2641 acgtggtacg cggtggtggt gcggtgcgtt acggaccgca gagcgtgggc ggcgtggtga
     2701 actttgttac ccgtgccatt ccgcaggact ttggtatcga ggcgggcgtg gaaggtcagc
     2761 tcagcccaac ctcttcacaa aacaacccga aagagacgca caacctgatg gtgggcggca
     2821 cagcggacaa cggttttggc accgcgctgc tctactccgg cacgcgcggc agtgactggc
     2881 gcgagcacag cgccacccgc atcgacgacc tgatgctgaa aagcaaatat gcgccggatg
     2941 aggtgcacac cttcaacagc ctgctgcaat attacgacgg tgaagccgac atgcccggtg
     3001 gcctgtctcg cgcggattac gacgccgatc gctggcaatc cacccgcccg tatgaccgct
     3061 tctggggtcg tcgcaagctg gcgagcctgg gctaccagtt ccagccagac agccagcata
     3121 aattcaacat tcaggggttc tacacccaaa ccctgcgcag cggctacctg gagcaaggca
     3181 aacgcatcac cctctcgccg cgtaactact gggtgcgcgg tattgagcca cgctacagcc
     3241 agatctttat gatcggccct tccgcgcacg aagtgggcgt gggctatcgc tatgtgaatg
     3301 aatcaacgca tgaaatgcgt tactacaccg ccaccagcag cgggcagttg ccgtccggct
     3361 caagccctta cgaccgcgat acgcgttccg gcaccgaggc gcacgcctgg tatctggatg
     3421 acaaaatcga catcggcaac tggaccatca cgccgggtat gcgtttcgaa catatcgagt
     3481 cataccagaa caacgccatc acaggcacgc acgaagaagt gagctataac gcaccgcttc
     3541 cggcgttgaa cgtgctctat cacctgactg acagctggaa tctttatgca aacactgaag
     3601 gctcgttcgg caccgtacag tacagccaga ttggcaaggc tgtgcaaagc ggcaatgtgg
     3661 aaccggaaaa agcgcgaacc tgggaactcg gtacccgcta cgacgacggc gcgctgacgg
     3721 cggaaatggg gctgttcctg attaacttta acaatcagta cgactccaac cagaccaacg
     3781 acaccgtcac tgcacgtggc aaaacgcgcc ataccgggct ggaaacgcag gcacgttacg
     3841 atctgggtac gctaacgcca acgcttgata acgtttccat ctacgccagc tatgcgtatg
     3901 tgaacgcgga aatccgcgag aaaggcgaca cctacggcaa tctggtacca ttctccccga
     3961 aacataaagg cacgctgggc gtggactaca agccaggaaa ctggacgttc aatctgaaca
     4021 gcgatttcca gtccagccag tttgcggata acgccaatac ggtgaaagag agcgccgacg
     4081 gcagtaccgg ccgcattccc ggcttcatgc tctggggcgc acgcgtggcg tatgactttg
     4141 gcccgcagat ggcagatctg aacctggcat tcggtgtgaa aaacatcttc gaccaggact
     4201 acttcatccg ctcttatgac gacaacaaca aaggcatcta tgcaggccag ccgcgcacgc
     4261 tgtatatgca ggggtcgttg aagttctgat gtgttaacgc ccggcttgcc gggcttttag
     4321 ctggaatgtg attatgttgg catttatccg ttttgttttt gcaggcctgc tgctggtgat
     4381 cagccacgcc tttgccgcca cggttcagga cgaacacggc acgtttacac tcgaaaaaac
     4441 gccacaacgg attgtggtgc tggaactctc gttcgccgat gcgctggccg ccgtggacgt
     4501 cagcccgatc ggtattgccg acgataacga tgcaaaacgc atcctgcccg aagtgcgtgc
     4561 gcacctgaaa ccgtggcagt ccgtcggaac gcgcgcgcag ccgagcctgg aagccattgc
     4621 cgctctgaaa ccagacctga tcattgccga cagcagtcgc catgcggggg tttacatcgc
     4681 cttgcagcaa atcgcgccgg tactgctgct taagtcccgc aacgaaacct acgctgaaaa
     4741 tttgcaatct gcggctatca tcggcgaaat ggtgggtaaa aagcgagaga tgcaggcacg
     4801 cctggaacaa cataaagaga ggatggcgca gtgggccagc cagcttccca aagggacacg
     4861 cgtggccttt ggcacatcac gcgaacagca attcaacctg catactcagg agacctggac
     4921 cggcagcgtg ctggcctctc tggggctgaa cgttcccgct gcgatggcgg gtgcgtccat
     4981 gccgtccatc ggcctggagc aactgctggc ggtcaatcct gcctggctgc tggttgccca
     5041 ctatcgcgaa gagagcattg ttaaacgctg gcaacaagat ccgctctggc agatgttaac
     5101 cgccgcgcag aagcagcagg ttgcttcggt cgacagtaac acctgggcgc ggatgcgcgg
     5161 tatttttgct gcagagcgta ttgccgctga cacggtaaaa atcttccacc atcagccgct
     5221 taccgttgtg aaatgaccgc gataaaacac ccggtgctgc tgtgggggct tcccgttgca
     5281 gcacttatta ttattttctg gctgagtctg ttttgctact cggccattcc tgtttccgga
     5341 gcagatgcaa cccgcgccct gctgcctgga cacacgccaa cgctaccaga agcgctggtg
     5401 caaaaccttc gtttgccacg aagcctggtc gccgttctga tcggcgcaag cctggcgctc
     5461 gcgggcacgc tgctgcaaac cctgacccac aacccaatgg cctctccttc actgctcggc
     5521 attaacagcg gcgcggcgct ggctatggcg cttaccagcg cgctgagtcc gacgccgatt
     5581 gcaggctatt ctctgtcgtt catcgcggca tgcgggggcg gcgtgagctg gctgctggtc
     5641 atgaccgcag gaggcggatt tcgtcatacc catgacagaa acaaactgat cctcgcgggt
     5701 atcgcgctgt cggccttttg tatgggcctt acccgcatca ccctgctgct ggccgaagat
     5761 catgcttacg gcatctttta ctggctggca ggcggagtgt cccacgcccg ctggcaggat
     5821 gtctggcagc tcttgccggt ggtggtcact gcagtccctg tcgtgttgct gctggcgaat
     5881 caactgaacc tgctcaacct cagcgacagc accgcccata cgctgggagt gaacctgacg
     5941 aggctacgtt tggtcatcaa tatgttagtg ctgcttctgg ttggtgcgtg cgtcagcgtg
     6001 gcaggtccgg tggcgtttat cggtctgctg gtgccacatc tggcgcgctt ctgggcaggc
     6061 ttcgatcagc gcaacgtact gccagtgagc atgctgctgg gggccacgct gatgctgctg
     6121 gcagatgtac tcgcacgcgc actggccttc cccggagatc tgcccgcagg cgcagtgctg
     6181 gcgctgattg gcagcccttg ctttgtctgg cttgtgagga ggcgaggatg aaaattgcgc
     6241 tggttatttt catcaccctt gccctggcgg gctgtgcgct gttatcactc catatgggag
     6301 tgatccccgt gccgtggcgc gcgctgctga ccgactggca ggccggacgc gagcattatt
     6361 atgtattgat ggagtaccga ctgccgcgct tgctgctggc actgtttgtc ggtgcagccc
     6421 tcgccgtggc gggcgtgctg atacagggga ttgtgcgcaa ccctctggca tcaccggata
     6481 ttctcggtgt taaccatgcc gccagcctgg cctctgtggg ggctctactt cttatgccgt
     6541 cactgcccgt gatggtgctg ccgctgctgg cctttgcggg cggcatggcg gggttgatat
     6601 tgctgaagat gctggcaaag acccaccagc cgatgaagct ggcgctcacc ggcgtggcgc
     6661 tttctgcatg ctgggccagc ctgacggatt atctgatgct ctcgcgcccg caggatgtga
     6721 acaacgccct gctgtggctg accggcagct tatggggccg tgactggagc tttgtgaaga
     6781 ttgccatccc gctgatgatt ttatttctgc cgctgagcct gagtttttgc cgcgatctcg
     6841 acctccttgc actcggcgat gcgcgcgcca ccacgctcgg tgtgtcggtg ccccataccc
     6901 gattctgggc tttgttacta gctgtcgcca tgacatctac cggcgtggcc gcctgcggcc
     6961 cgattagctt tattggtctc gtggtgccgc atatgatgcg tagcatcacc ggtggacgtc
     7021 accgcagact gctgcctgtt tcggccctga caggtgcgtt gctgttggtg gttgccgatc
     7081 tgctggcgag aattattcat cccccactgg agctcccggt tggcgtgctg accgccatta
     7141 tcggtgcgcc gtggtttgtc tggttgcttg tgagaatgcg ataaatgact ttacgaactg
     7201 aaaatctgac ggtcagttac gggacagaca aggtacttaa cgacgtttca ctctcactgc
     7261 caacggggaa gatcaccgcc ctgatcggtc ctaacggttg cgggaaatcg acgctgttaa
     7321 actgtttttc gcggctttta atgccgcagt ctggcaccgt atttctcggc gataatccca
     7381 taaatatgct ctcatcgcgc cagttggccc gcaggctttc gctgctgcct cagcaccatt
     7441 taacgccaga ggggatcaca gtccaggagc tggtttcgta tggtcgtaat ccctggctgt
     7501 cactctgggg gcgtctctcc gctgaagaca atgcacgagt taatgtcgcc atgaaccaga
     7561 cccggatcaa tcatcttgcc gttcgtcggt taaccgagct ttccggcggt cagcgccagc
     7621 gcgcatttct ggcaatggtc ctggcccaga atacgcccgt tgtattactt gatgagccaa
     7681 ccacctatct tgatatcaat caccaggtgg acctgatgcg gttgatgggc gaactccgga
     7741 ctcaggggaa aacggtggtc gctgtgctgc acgaccttaa tcaggctagc cggtactgcg
     7801 atcaactggt ggtaatggca aacggacatg ttatggcgca aggcacacca gaagaggtga
     7861 tgaccccagg attgctgaga acagtattca gcgtggaagc ggaaatacac cccgagccgg
     7921 tatctggcag gccgatgtgc ctaatgaggt agattgcaca ggccgtaaga accaaaccac
     7981 gactgaatga aactggactg gcgccagcaa gcctgttcag actggggctg aacttttccg
     8041 gactctgaaa gattaccaat actcatcgtc catccgcttg ctttaggctg acaggttcat
     8101 aatcaacgca aaccagagct gtacaggctt gggcgcggct ttcaaaccag tcgtgatcac
     8161 ggcaatcaat tttgaactct gcttaacgga catttctgta taacccttac ggcaacgaaa
     8221 aacgcgaagt taaaatttta gaaacccaaa aacgtgacat gactaagttt agatttcagg
     8281 ggggagatca aaaaatttcg ctctgtgcca gagcggacat tcacggagct ggttcattac
     8341 caatgaggtt gggcttttga ggataaatca atgatcagac gccaacgtaa atcaaaagca
     8401 accctggaac ggaattgcta atccagtttc tgaccatcga tttttctaaa aagtgtgcgt
     8461 ttgctactac ttaggtaggt gcagctttct taatcaccgg cagccacgtt atacaggcca
     8521 gttgatggat cgattgttat caatgatatc ttcatgagtc ggtgtctcac ccagcttacc
     8581 gaagctggca taaagtgaag gcagacgggc ccgtccttct ccctttttcg ccagagggaa
     8641 agcgcgaagc atggtggcag cctcccagtc acaacaatag gatggtgtgc acggctgctg
     8701 acgccatgat tcagcgatag agccggaaaa tacggggtca aagccggtat cattaaccag
     8761 agtcatcgtg acctgtactg cggctggatg atctcctgct actgcaactg ccaggcgacc
     8821 gcggctccct tcaggtagtc tgttgtggtc aactaaaact ggcctccgcg ttagagtttt
     8881 tccagtatcg gttttctgat tcgtttggtg gtaacccacc attatattcg tgcggtctta
     8941 gtgcgctgta atatccaacg atatagtccg ttattgcgtg agctgcatcg ctgaagctta
     9001 catagcccgt cgctggcacc cattcgttct tcagactcct gaagaagcgc tccattgggc
     9061 tgttatccca gcagtttcca cgccgactca tactctgcct gatccggtat cgccacagta
     9121 actgccggaa ctgcctgctc gtataatgac tgccttgatc gcttggaaca tcaccccgac
     9181 gggcttacca cgggtttccc atgccatttc cagtgctttc atggt
//
LOCUS       DBANUU010000159         9196 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000159 DBANUU010000000
VERSION     DBANUU010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.161"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..162
                     /locus_tag="ACIE68_004001"
     CDS             <1..162
                     /locus_tag="ACIE68_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFX5910238.1"
                     /translation="DECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGG
                     FLSINKLSQ"
     gene            168..911
                     /locus_tag="ACIE68_004002"
     CDS             168..911
                     /locus_tag="ACIE68_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFX5910239.1"
                     /translation="MTKTESAILAHARRCAPAESCGFVVKTPEGDRYLPCVNISGEPE
                     AYFRMSPEDWLSAEMQGEIVSLVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRRAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHQAWHASAFTGIYNDLAAASICV"
     gene            848..1450
                     /locus_tag="ACIE68_004003"
     CDS             848..1450
                     /locus_tag="ACIE68_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFX5910240.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALAMQIPVFRQKLSD
                     GWYQVRIAGRDTGETELSARLNEPLANGAVIHIVPRLAGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            1511..4990
                     /locus_tag="ACIE68_004004"
     CDS             1511..4990
                     /locus_tag="ACIE68_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFX5910241.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSEAVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQGYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRIACNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKYLTAPTITSGGNP
                     PAFSLTPDGKLTAKNADISGSVNANSGTLNNVTINENCQIKGKLSANQIEGDIVKTVG
                     KAFPRDSRAPERWPSGTITVRIYDDQPFDRQIVIPAVAFCGAKHERENNDIYSSCRLI
                     VKKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNDWYPTASISDLLV
                     VVMKKATAGISIS"
     gene            5058..5657
                     /locus_tag="ACIE68_004005"
     CDS             5058..5657
                     /locus_tag="ACIE68_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFX5910242.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWYEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGNYLQVTDNTGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTNSFIVGVGYKF"
     gene            5722..8682
                     /locus_tag="ACIE68_004006"
     CDS             5722..8682
                     /locus_tag="ACIE68_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFX5910243.1"
                     /translation="MAVRISGVLKDGAGKPIQNCTIQLKARRNSTTVVVNTVASENPD
                     EAGRYTMDVEYGQYSVSLLVEGFPPSHAGIITVYEDSKPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDARTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKASEASKSAAAAESSKSAAATSAGAAKTSETNAAASQKSA
                     ATSASAATTKASEAATSARDAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTNAGEAAVQASAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAARGSATTASTKATEAAGSAAAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGVVQLSSATNSTSESQAATPKAVKAAYDLANGKYTAQDATTG
                     QKGIVQLSSATNSTSESQAATPKAVKAAYDLANGKYTAQDATTAQKGIVQLSSATNST
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTLTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQAFDKSAYPKLAAAYPSG
                     VIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTN
                     NTGAHTHSLSGSTNAAGNHSHRDGRRFNPSVFKDTYQYGYTSSGQNTWGVQGSVGIST
                     GWLANTSTDGNHSHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAEN
                     TVKNIAFNYIVRLA"
     gene            8682..>9196
                     /locus_tag="ACIE68_004007"
     CDS             8682..>9196
                     /locus_tag="ACIE68_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFX5910244.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDKASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRV"
BASE COUNT         2248 a         2297 c         2765 g         1886 t
ORIGIN      
        1 gatgagtgcg gttatcacgg tccggcggtc gcggatgaat atgaccagcc aacgtccgat
       61 atcacgaagg ataaatgcag caaatgcctg agcggttgta agttccgcaa taacgtcggc
      121 aactttggcg gcttcctttc cattaacaaa ctttcgcagt aaatcccatg acaaagacag
      181 aatcagcgat tctggcgcac gcccggcgat gtgcgccagc ggagtcgtgc ggcttcgtgg
      241 tgaaaacgcc ggagggagac agatatcttc cttgcgtaaa tatctccggt gagccggagg
      301 cgtatttccg gatgtcgccg gaggactggc tgagtgcaga aatgcagggt gagattgtct
      361 cgctggtcca cagtcacccc ggtggtctgc cctggctgag tgaggccgac cggcggctgc
      421 aggtacagag tgatttgccg tggtggctgg tctgccggag ggcgattcac aagttccgct
      481 gtgtgccgca tcttaccggg cggcgctttg agcacggggt gacggactgt tacacgctgt
      541 tccgggatgc ttaccatctg gcgggaattg agatgccgga ttttcatcgc ggggatgact
      601 ggtggcgtca cggtcagaat ctctatctgg ataatctgga ggccacaggg ctgtatcagg
      661 tgccgttgtc ctcagcacaa ccgggcgatg tgctgctgtg ctgctttggt tcatcggtgc
      721 cgaatcatgc cgccatttac tgtggtgacg gcgagctgct gcaccatatt cctgaacaac
      781 tgagcaaacg agagaggtat accgacaaat ggcagcgacg cacacactcc ctctggcgtc
      841 accaggcatg gcacgcatct gcctttacgg ggatttacaa cgatttggcc gccgcatcga
      901 tctgcgtgtg aaaacggggg ccgaagccat ccgggcgctg gccatgcaga tcccggtgtt
      961 tcgtcagaag ctgagcgacg gctggtatca ggtacgcatt gccgggcgtg atacaggtga
     1021 aacggaatta tcagcccgtc ttaatgaacc gctggcaaat ggtgctgtga tccatatcgt
     1081 gccgcgtctg gcgggagcca aaagtggcgg tgtgtttcag gctgtgctgg gggcggctgt
     1141 tatggcggtt gctatatgga tgccgggggt aggaattatg gcgagtaatc tgctgttttc
     1201 tctcggtgcc agtatggtgc tcggtggtgt ggcgcagatg ctggcaccga aagccagaac
     1261 cccccgcaca cagacaacgg ataacggcaa acagaacacc tatttctctt cactggataa
     1321 catggttgcc cagggcaatg ttctgcctgt tctgtacggt gaaatgcgcg tggggtcacg
     1381 cgtggtttct caggagatca gcacggcaga cgaaggggat ggtggtcagg ttgtggtgat
     1441 tggtcgctga tgaaaaacgt tttatgtgaa accgcctccg ggcggtttta tcgtttatgg
     1501 agcatgacga atgggtaaag gcagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa
     1561 cctgaagtcc acgcagctgc tgagtgtgat cgatgccatc agcgaagggc cggttgaagg
     1621 tccggtggat ggattaaaaa gcgtgctgct gaacagtacg ccggtgctgg acagtgaggg
     1681 gaataccaac atctccggcg tcacggtggt gttccgggcc ggtgagcagg agcagacacc
     1741 gccggaggga tttgaatcct ccggctccga ggcggtgctg ggtacggaag tgaaatacga
     1801 cacgccgatc acccggacca tcacgtcggc aaacattgac cgactgcgtt ttaccttcgg
     1861 cgtgcaggca ctggtggaaa ccacctcaaa gggggacagg aatccgtcgg aagtccgcct
     1921 gctggttcag atacaacgta acggtggctg ggtgacggaa aaagacatca ccattaaagg
     1981 caaaaccacc tcacagtatc tggcctcggt ggtggtgggt aacctgccgc cgcgcccgtt
     2041 caatatccgg atgcgcagga tgacgccgga cagcaccaca gaccagttgc agaacaaaac
     2101 gctctggtcg tcatacaccg aaatcatcga tgtgaaacag ggctacccga acacggcact
     2161 ggtcggcgta caggtggatt cggagcagtt cggcagccag caggtgagcc gtaattatca
     2221 tctgcgcggg cgtattctgc aggtgccgtc gaattataac ccgcagacgc ggcaatacag
     2281 cggtatctgg gacggaacgt ttaaaccggc atacagcaac aacccggcct ggtgtctgtg
     2341 ggatatgctg acccatccgc gctacggcat gggaaaacgt cttggtgcgg cggatgtgga
     2401 taaatgggcg ctgtatgtca tcggccagta ctgcgaccag tcggtgccgg acggctttgg
     2461 cggcacggag ccgcgcatcg cctgtaatgc gtacctgacc acacagcgaa aggcgtggga
     2521 tgtgctcagt gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct
     2581 gacattcgtg caggaccgac cgtcggataa ggtgtggacc tataaccgca gtaatgtggt
     2641 gatgccggat gatggcgcgc cgttccgcta cagcttcagc gccctgaaag accgccataa
     2701 tgccgttgag gtgaactgga ttgacccgaa caacggctgg gagacggcga cagagcttgt
     2761 ggaggatacg caggctattg cccgttacgg tcgtaacgtc acgaagatgg atgcttttgg
     2821 ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg ctgattaaaa cagagctgct
     2881 ggaaacgcag accgtggact tcagcgtggg cgcagaaggg cttcgccatg tgccgggcga
     2941 tgttattgaa atctgtgatg atgactatgc cggtatcagc atcggcgggc gcgtgctggc
     3001 ggtaaacagc cagacccgga cgctgacgct cgaccgtgaa atcacgctac catcctccgg
     3061 taccacgctg ataagcctgg ttgacggaag tggcaatccg gtcagcgtgg aggttcagtc
     3121 cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct gacggtgttg ccggatacag
     3181 cgtgtggggg ctgaagttgc cgacgctgcg tcagcgcctg ttccgctgtg tgagtatccg
     3241 tgagaacgat gacggcacgt atgccatcac tgccgtgcag catgtgccgg aaaaagaggc
     3301 catcgtggat aacggggcgc actttgacgg tgaccagagc ggcacggtga atggtgtcac
     3361 gccgccagca gtgcagcatc tgaccgccga agtcactgca gatagcgggg aatatcaggt
     3421 gctggcgcgc tgggacacac cgaaggtggt gaagggcgtg agcttcctgc tccgtctgac
     3481 cgtgacagcg gatgacggca gtgagcggct ggtcagcaca gccaggacga cggaaaccac
     3541 ataccgcttc acacaactgg cgttggggaa ctacaggctg acagtccggg cggtaaatgc
     3601 gtggggacag cagggcgatc cggcatcggt atcgttccgg attgccgcac cggcagcgcc
     3661 gtctcggatt gagctgacac cgggctattt tcagataacc gccacgccgc atcttgcggt
     3721 ttatgatccg acggtacagt ttgagttctg gttctcggaa aagcggattg cggatatcag
     3781 gcaggttgaa accacagcac gctatcttgg cacggcgctg tactggatag ccgccagtat
     3841 caatatcagg ccgggccatg attattattt ttacgttcgc agtgtgaaca ccgttggcaa
     3901 atcggcattc gtggaggctg ttggtcagcc gagtgatgat gcatccggct atctggattt
     3961 tttcaaaggc gagataggga aaacccatct ggctcaggag ctgtggacgc agattgataa
     4021 cggtcagctt gcgcctgacc tggctgaaat caggacgtcc attacggatg tcagcaatga
     4081 aatcacgcag accgtcaata agaaactgga agaccagagt gcggcaattc agcagataca
     4141 gaaggttcag gttgatacaa ataataacct gaacagcatg tgggctgtga agctgcagca
     4201 gatgcaggac ggacgccttt atatcgcggg tattggtgcc ggtattgaga acacccctga
     4261 cggcatgcag agtcaggtgc tgctggcggc ggacaggatt gcgatggtta atcctgcgaa
     4321 tggcaacaca aaaccgatgt ttgttggtca gggcgatcag atattcatga acgacgtgtt
     4381 cctgaagtat ctgacggccc ccaccattac cagcggtgga aatccaccgg cattttccct
     4441 gacaccggac ggaaagctga ctgctaaaaa tgcggatatc agtggcagtg tgaatgcgaa
     4501 ctccgggacg ctcaacaacg tcacgattaa tgagaactgt cagattaagg ggaaactgtc
     4561 agccaatcag attgaaggcg atattgtcaa aacagtgggt aaggctttcc cgcgggactc
     4621 ccgggcaccg gagcggtggc catcagggac cattaccgtc aggatttatg acgatcagcc
     4681 ttttgacagg caaattgtta ttccagcggt ggcattctgc ggcgctaaac atgagcggga
     4741 gaataacgat atttattcgt catgccgcct gatagtgaag aaaaatggtg ctgaaattta
     4801 taaccgtacc gcgctggata atacgctgat ttacagtggt gttattgata tgccagctgg
     4861 tcacggccac atgacgctgg agttttcggt gtcagcatgg ctggtaaatg actggtatcc
     4921 cacagcaagt atcagcgatt tgctggttgt ggtgatgaag aaagccaccg caggcatcag
     4981 tatcagctga attttataac ccatatacgg gcgccagaaa tggcgccttt tttattgcag
     5041 aaaagcgaga ggtaattatg cgtaaagttt gtgcagccat tttgtccgca gccatctgtc
     5101 tggccgtatc cggtgcgcct gcatgggcgt ctgagcagca ggccacactg agcgcagggt
     5161 atcttcatgc ccgtacgaac gctcccggca gcgataatct gaacgggatt aacgtgaaat
     5221 accgttatga gtttacggac acgctggggc tgattacgtc attcagttat gccaacgctg
     5281 aagatgagca aaaaacgcat tacagcgata cccgctggta tgaagattcc gtgcgtaacc
     5341 gctggttcag cgtgatggcg gggccgtctg tgcgcgtgaa tgaatggttc agcgcgtatg
     5401 cgatggcggg tgtggcttac agccgtgtgt cgactttctc cgggaattat ctgcaggtga
     5461 ccgacaatac ggggaaaacg cacgatgtgc tgaccggaag tgatgacggt cgccacagca
     5521 acacgtctct ggcgtggggg gctggcgtgc agtttaaccc gaccgaatcc gtggccgttg
     5581 atgtcgctta tgaaggctcc ggcagcggtg actggcgtac caacagtttc atcgttggtg
     5641 tcggttataa attctgatta gccaggtaac acagtgttat gacagcccgc cggttcaggc
     5701 gggctttttt gtggggggaa tatggcagta cggatttcag gtgtactgaa agatggcgca
     5761 ggtaagccga tacaaaactg caccattcag ctaaaggcca ggcgcaacag caccacggtg
     5821 gtggtgaaca cagtggcctc agaaaacccg gatgaagccg ggcgttacac aatggacgtc
     5881 gagtatggtc agtacagcgt cagtctgttg gtggagggat tcccgccgtc acacgccggg
     5941 attatcaccg tatatgagga ctcaaagccg ggcacactga atgatttcct cggtgcaatg
     6001 acggaggatg atgtccggcc agaggcactg cgccgttttg aactgatggt ggaagaggtg
     6061 gcgcgtaacg cgtccgcagt ggcacagaac acggcagccg cgaagaagtc agccagcgat
     6121 gcccgcacat cagcccgtga ggcggcaacc catgcgactg atgctgcagg ctcagcacgc
     6181 gcagccagca cgtcagccgg acaggccgcg tcgtcggctc agtcagcgtc ttccagcgca
     6241 ggaacggcat caacaaaggc cagtgaagca tcgaaaagtg ctgctgctgc agagtcctca
     6301 aaaagcgcgg cagctaccag tgccggtgcg gcgaaaacgt cagaaacgaa tgcggcagcg
     6361 tcacaaaaat ctgcagccac ttctgcatcc gccgcgacca caaaggcgtc agaagctgcc
     6421 acctcagccc gggatgcggc ggcctcaaaa gaggcagcga aatcatcaga aacgaacgca
     6481 tcatcaagcg ccagtagtgc cgcttcctcg gcaacggcgg caggaaattc cgcgaaggcg
     6541 gcaaagacgt ccgagacgaa cgccaggtct tctgaaacgg cagcgggaca gagtgcctca
     6601 gctgcggcag gctcaaaaac agcggctgca tcatctgcca gtgccgcgtc aacaagtgcc
     6661 gggcaggcct cagccagtgc caccgctgcc ggaaagtcgg cagaaagtgc cgcatcgtct
     6721 gcttcaacag ccacaacgaa tgctggcgaa gccgctgtac aggccagcgc agcagcgagg
     6781 tcagcttccg cagcgaagac atccgaaacg aacgcgaaag cgtcggaaac cagcgcagaa
     6841 tcctcaaaaa cggctgccgc atcgtccgcc agttcggcgg cgtcatcggc atcatctgcg
     6901 tctgcttcaa aagatgaggc gaccagacag gcgtcagcag cgaggggcag cgccacgacg
     6961 gcatccacga aggcgacaga ggctgctggc agtgcggcag cggcagcaca gagcaaaagt
     7021 acggcagaat ccgcagcaac gcgcgctgag acagcggcaa aacgtgcaga ggatattgca
     7081 tccgccgtgg cgcttgagga tgcgagcacg acgaaaaagg gggtagtaca gctcagcagt
     7141 gcgaccaaca gcacttccga gtcacaggcg gcaacgccaa aagccgttaa ggccgcgtat
     7201 gacctggcta acgggaaata caccgcacag gatgcaacga caggacagaa agggatagtt
     7261 cagctcagca gtgcgactaa cagcacttcc gagtcacagg cggcaacgcc aaaagccgtt
     7321 aaggccgcgt atgacctggc taacgggaaa tacaccgcac aggacgctac gacagcacaa
     7381 aaaggaattg tccagctcag tagtgcaacc aacagcacat ctgaaacgct tgctgcgaca
     7441 ccgaaagcag tgaaagcagc taatgacaat gcgaatggtc gggtaccttc tgcccgtaag
     7501 gtgaatggta aggcgctttc agcggatata acactgacgc cgaaagatat tggtacgctt
     7561 aactcaacaa caatgtcatt cagcggtggt gctggttggt tcaaattagc aacggtaacc
     7621 atgccacagg cgagttctgt tgtttcaatt acgttgattg gtggcgctgg atttaacgtg
     7681 gggtcacctc aacaggcagg tatatctgaa cttgttttac gtgcaggtaa tggtaatccg
     7741 aaggggatta ctggtgcttt atggcagcgc acattgacag ggtttacaaa ttttgcctgg
     7801 gttaatacat ctggtgatac ttacgatatt tacgttgcaa tcggaaatta tgcgactggt
     7861 gtaaatattc aatgggatta taccagtaat gccagcgtga cgattcatac gtcaccagca
     7921 tattctgcta ataagccgga agggttaacg gacggtacag tttattcact ctatacgcca
     7981 tcagagcagt tttatccgcc tggcgcacca atcccgtggc catcagatac cgttccgtct
     8041 ggctatgccc tgatgcaggg gcaggctttt gacaaatctg catacccgaa acttgcagcg
     8101 gcttatccgt caggcgtgat ccctgatatg cgtggctgga cgattaaggg caaacctgcc
     8161 agtggtcggg ccgtattgtc tcaggaacag gacggcatta aatcgcatac ccacagcgcc
     8221 agcgcatcca gtacggattt ggggacgaaa accacatcgt cgtttgatta cggcactaaa
     8281 tccacgaata acactggtgc gcatacccat agtttaagtg gcagcacgaa tgcagctggt
     8341 aatcacagcc atagagatgg ccgtcgattt aaccccagtg tttttaaaga tacttatcaa
     8401 tatggttata caagctcagg tcaaaatacc tggggtgtac aaggctcagt aggtatatct
     8461 acggggtggt tagcgaatac cagtacagat ggtaatcata gccattcact gtccggcaca
     8521 gcagcatctg caggtgcaca cgcgcatact gtcggtattg gtgctcatac gcactccgtt
     8581 gcgattggtt cacatggaca caccatcacc gttaacgctg ctggtaacgc ggaaaacacc
     8641 gtcaaaaaca tcgcatttaa ctatattgtg aggcttgcat aatggcattc agaatgagtg
     8701 aacaaccacg gaccataaaa atttataatc tgctggccgg aactaatgaa tttattggtg
     8761 aaggtgacgc atatattccg cctcatacag gtctgccagc aaacagtacc gatattgcac
     8821 caccagatat tcctgctggc tttgtggctg ttttcaacag tgataaggca tcgtggcatc
     8881 tcgttgaaga ccatcggggt aaaacggttt atgacgtagc gtcaggggac gcgttattta
     8941 tttctgaact cggtccgtta ccggaaaatg ttacctggtt atcgccggaa ggggagtttc
     9001 agaagtggaa cggcacagcc tgggtgaaag atgcagaagc agaaaaactg ttccggatcc
     9061 gagaggcgga agaaacaaaa aacagcctga tgcaggtagc cagtgagcat attgcgccac
     9121 ttcaggatgc tgtagatctg gaaatcgcaa cggaggaaga aacctcattg ctggaagcct
     9181 ggaaaaagta tcgggt
//
LOCUS       DBANUU010000160         8963 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000160 DBANUU010000000
VERSION     DBANUU010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.162"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            115..1893
                     /gene="ygeV"
                     /locus_tag="ACIE68_004008"
     CDS             115..1893
                     /gene="ygeV"
                     /locus_tag="ACIE68_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFX5910245.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1933..2412)
                     /gene="xdhC"
                     /locus_tag="ACIE68_004009"
     CDS             complement(1933..2412)
                     /gene="xdhC"
                     /locus_tag="ACIE68_004009"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFX5910246.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2409..3287)
                     /gene="xdhB"
                     /locus_tag="ACIE68_004010"
     CDS             complement(2409..3287)
                     /gene="xdhB"
                     /locus_tag="ACIE68_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFX5910247.1"
                     /translation="MFDFASYHRAATLADAINLLVDNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPVTQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3298..5595)
                     /gene="xdhA"
                     /locus_tag="ACIE68_004011"
     CDS             complement(3298..5595)
                     /gene="xdhA"
                     /locus_tag="ACIE68_004011"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFX5910248.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            6010..6765
                     /gene="actS"
                     /locus_tag="ACIE68_004012"
     CDS             6010..6765
                     /gene="actS"
                     /locus_tag="ACIE68_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFX5910249.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSITRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6844..6917
                     /locus_tag="ACIE68_004013"
     tRNA            6844..6917
                     /locus_tag="ACIE68_004013"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6876..6878,aa:Gly,seq:ccc)
     gene            7151..8761
                     /locus_tag="ACIE68_004014"
     CDS             7151..8761
                     /locus_tag="ACIE68_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFX5910250.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHVKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPNP
                     IIPLNWSKASMTLMLKEMSSLINDEGNK"
     gene            8828..>8963
                     /locus_tag="ACIE68_004015"
     CDS             8828..>8963
                     /locus_tag="ACIE68_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HFX5910251.1"
                     /translation="MINKLLLAYLIGLVVTSTFIFIFSEEKVTYRLFATIITGLTWPL
                     S"
BASE COUNT         2406 a         2050 c         2203 g         2304 t
ORIGIN      
        1 taaaaatctt atgtttttat caaagccaat ttcgcctgag ttaatatcat catgatctgt
       61 gaaccatcaa cgtcttcaat gatcgacttt taagtaagtc tggaaggtaa agctatggag
      121 cttgctacta cgcagtcagt attgatgcaa attcaaccga caattcagcg ttttgccaga
      181 atgcttgcca gcgttttgca gcttgaggtt gagatcgttg atgaaaactt gtgtcgcgtc
      241 gccggaacgg gcgcgtatgg gaagtttctt ggtcgccagt tgagcggcaa ctcacgtctg
      301 ctccgccacg tcctggaaac gaaaactgaa aaagttgtga cacagtctcg cttcgatccc
      361 ctttgcgaag gttgcgatag taaagaaaat tgccgcgaaa aagcatttct gggtacgcct
      421 gtcattttac aggatcgttg tgttggggtg ataagtttga ttgccgttac ccacgagcaa
      481 caagagcata tcagtgataa tttacgcgaa ttttctgatt atgttcgcca tatatccacc
      541 atttttgttt cgaaacttct ggaggatcag gggccaggag ataacatcag taaaatattc
      601 gcgaccatga tcgataatat ggatcagggc gtattagttg ttgatgatga aagtcgggtt
      661 cagtttgtta atcagactgc cttaaaaaca cttggtgttg tacaaaataa tattattggg
      721 aaacctatcc gtttcagacc attaacattt gagagtaatt ttactcatgg acatatgcag
      781 catattgttt cgtgggacga taaaagtgaa ttaatcattg gtcaattgca taacattcag
      841 ggccgacaat tatttttaat ggcatttcac caatcgcata ccagtttttc tgtagcaaat
      901 gcacctgatg aaccacatat tgaacaattg gttggcgagt gccgtgttat gcggcaatta
      961 aaacgactca ttagccgtat tgcacccagc ccatccagcg ttatggtggt tggtgaaagc
     1021 ggcacgggta aagaagttgt cgcccgtgca atccataagt tgagcggaag acggaataaa
     1081 ccctttattg ctatcaactg tgccgcgatt ccggagcagc ttctggagag cgaactgttc
     1141 ggttatgtta aaggcgcatt tactggcgct tctgccaacg gtaaaacagg gttgattcag
     1201 gcggcgaata cgggcacgct gtttctcgat gaaataggtg atatgccatt aatgttgcag
     1261 gctaaattac tgcgcgctat tgaggcacgt gaaattctgc cgattggtgc cagtagccca
     1321 atacaagtcg acattcgcat catttctgca actaaccaga atttggccca gttcattgcc
     1381 gaaggtaaat tccgcgaaga tctcttctac cgacttaatg ttatcccgat aactctgcca
     1441 ccgctgcgtg aacgtcagga agatattgaa ctactggtgc attacttttt acatctgcat
     1501 acccgtcgtc tgggatcggt ttatcctggc attgctcccg atgtcgtcga aatattgcgt
     1561 aagcatcgtt ggcccggaaa cctgcgcgag ttaagcaatt tgatggaata tctggttaac
     1621 gttgttcctt caggtgaagt tatcgacagc acgctattgc cgccaaatct gctgaataat
     1681 ggcacaacgg agcaaagtga tgtaacagag gtcactgagg cacacctgtc actcgatgat
     1741 gcgggcggca cggcgctgga ggagatggaa aagcaaatga tccgcgaggc gctttcacgt
     1801 cataacagca agaagcaagt tgctgatgaa ctgggcatcg gcattgctac gctctatcgc
     1861 aagattaaga aatatgagtt gttaaacaca taacctcagt gaatccggcc tgaattcagg
     1921 ccggatatcc ttttacttcg ttttctcgca atccagaacc gtatttacaa tcatctgata
     1981 ccccgtgcag cgacaaagat ttcccgccag tccgcgacga atttccgtaa tggttaatgg
     2041 cttctcgcgt ggtttcgcca gcattgccgt ggtagccata atcaggccag gcgtacaaaa
     2101 cccgcactgc accgcgccgg atttcgcata agcctgctga acatgagaaa gttttccgcc
     2161 tttcgcttca ccttcaagcg tgcggatctc ttttccttca gcccaggcgg caaggtataa
     2221 gcaactgtct attgctgtgc cgtcgaccag caccgtacag gcaccacatt cacccacgca
     2281 gcacccttgt ttgacactta gcagtccttg ttcgcggagt aattccgaga gcggcgtacc
     2341 tggtgcggca tgaagctgaa aaggcatccc gttaatggtg cattcgatgg taattgtttc
     2401 gctgtgattc attgcaattt tccccccgcc gcggcgacgg cttcgctaat cacttttttg
     2461 gtcatcgtct ggatgagatg cagacgaaac tctttactgg cccgccatga agaacgcggg
     2521 gcgacatctt gcaggacaga ttcgctgata gcttccagcg tttgcaggtt taatggcgca
     2581 ttttgtgcag tctgttcggc atgttggcag cgaatcggcg ttggcgcggc aacaccaaat
     2641 gccaggcgta attcgctgaa attgccgtta tccagtcggc aatgtgcggc acagccaatc
     2701 gttgaaatat ccattgcgtc gcgcatggca tatttaaaat gcgcgctgcc cacgtgttct
     2761 ttcggctggg gcggaaaatg aaaggcgaca aggatttcgt catgctcaag agacactttg
     2821 cctggcccag tgtgaaagcc attaatcggg acgaaacgaa caccgcgtgg ggagtggatc
     2881 tccagctttg cgtcataaat tagcgttggc gtggcagaat ctgcgctggt ggcaccgttg
     2941 caaatatttc caccgtaggt agcgacgtta cggatctgcg gtccggcaat ggatgaggcc
     3001 gcagcacata acgccgggag atgacgttga gttacaggat cttcaatgag ctgggtaaat
     3061 gtcgttgcag agccgatacg tagtgagcca tcttccgcca gcgtaattcc ccgcagctcc
     3121 gccagattat gaatatcaac aatatggcga taacggtcat tgtgatggtg gagctgaatc
     3181 agtacgtcag tgccaccggc gagcagtttg gcctgcgggt tgtcaaccag caggttgatg
     3241 gcatcggcaa gggttgctgc gcgatggtaa gaagcaaaat caaacatgat gttatcctca
     3301 aatcaatcct gccagatgga actcttcata taaccgtttt ggcgtcagcg gcagtgtatt
     3361 gattgcaaca ccggtagcca tcttcaccgc gttacgaata gctgcggcaa caggaattat
     3421 tggtggctca cccagtgact tatgtccgta tgcggattgc ggctcattga tttcgacgaa
     3481 cgcgctttcc agttgtggca gatccggcat ggtcggcatt ttgtaatcca gcagattggg
     3541 gttacggacc acgccgcttt tcgcatcgat gatcatctct tcaaatagcg cccagccaat
     3601 gcccattccc attccgccgt gtacctgacc ttctgccagc agcggattta gaatatgccc
     3661 cgaatcatga acgttgagga tgcggttgat ggtgactttg cacagtgcaa tatcgaccgt
     3721 cagatcaaca aaggtacagc cgaacgccgg tgggttagtg gtggttttga tggagctttc
     3781 agcagagagc tgcccgccgc gttcagggtg gtagaaagcg tccatcgcca aatcttttaa
     3841 cgacattaac ggctcttccg gtcgttcaac cagcacgata tggcctttta tcagggtcag
     3901 attcatcgct gactgatgta gcatgactgc ggcgtgagcg atgattttct cttttaataa
     3961 cagtgccgca ctgcgcagcg caggcgcggc aacatagctc tggcgcgagg caaatgcgcc
     4021 gggatcgaac ggcgtaacgt cggtatcttg tgttgaaata acgcgaacgt cgctgaccgg
     4081 aacccccacg gtttctgcca ccatttgcga gaagacggtg tcggcaccct gaccgatttc
     4141 cgtcgcgccg ctttgcacgt tgatggttcc atcctgattc atcagaaggc gcgcgcctgc
     4201 tatttctacg ccgacaggcc aggtattaga ggtgtagcta aaacaggcga cgccaacgcc
     4261 gcggcgtaaa ttgccttgct ggttctggca ttctgcacgg cgtttttccc attcaaagat
     4321 tttccggcct ttttcaagac actccggcaa ccctgcgctg taaatacgtt tgcccgtgag
     4381 cggattagca tctccttcgc gggcggcgtt gcgtaaacga atttcaacag gatcaatacc
     4441 taacgctgtc gcggcgtcat caagcataga ctcaacggca aatacgactt gtggcgcgcc
     4501 ataaccacgc atcgcaccag ccgaggggag gttggtatag caggtctttg aactgtaagc
     4561 gtaggcacaa cgaggataaa ggtaagcgac tttattcccc cccgcagaag cgatggagtg
     4621 cccgtgagat gcataagcgc cggtgttaga cagaacatcc agactataac ctttcaatgt
     4681 tccgtcgcgg ttcacgccca tttgcccgtc aatagtaaaa gcgtggcggg tacgggttgc
     4741 gaggaaacac tcttcacggc taagggaaac tttcaccgga atgccgccaa gcttgctggt
     4801 caggaatgcc gccattggct cttccagtac atcctgttta ttaccaaaac cgccaccgac
     4861 aaatggtttg atgactcgta cgcatgacca gggaatatcc agcgcctgac caaccacgcg
     4921 gcgaacaatg tgcgggatct gggtgctgga aacgatggta attcgcgagt catcctccat
     4981 ccacgccagc gatgttacgc tttccatgtg acaatgttga ataacggggg tctgatagtg
     5041 cccctgtacc tggtagtcgg cggcatcgat tgtttgttgg acattgcccg tcgacatcgt
     5101 gctttgtttc agtaaattgc cgccgttatg gattggtgca gcgtcctctg ccagcgccgc
     5161 ttctggcgtg gtgataacgg gtaattcttg ccactcaatg ctgaccaatt gcgccgcttt
     5221 ttctgccgtg agttcatcgc gggccacgac gatggcaacg gcgtcaccat gatgacgaac
     5281 atggcgagtt agcagtgcgc gatcggcggt atcgcgcttg ttttcgtcaa gtgtccatgc
     5341 atgtccagct gtagcgaatg ggatatcagg cacatcttcc caggtaaaaa tcgccagtac
     5401 gcccggtaaa cttctggctt gttcatcatt aatacttacg gcataaccat gtgcgatagg
     5461 gctacgtaca tatttcgcat aacacatgcc cgccataaca taatcgtcag tatatcgtgc
     5521 ccgcccggtg accttagcaa tggcatcgac gcgcatgcat gattcacccg tagcggttgc
     5581 ttcccgcgct tccatagaaa tcccctcgat ttgaacattt ggttcttatg gcaaagattt
     5641 agtctaagca ggcaagattt acgccagaaa cgacgataag cgttagggga ggaaggaagt
     5701 gtgatgacgt taaaaaaaca cagatatgat acgaaacagg actctgaata gggacagtga
     5761 tacgtatcaa tatgagtggc gataaaatca atgtgataaa tcaggattat tacgcatcag
     5821 gatgggaaat agggtaaaaa gtcaggaact tcgatacctc tacagcgaat attttgctga
     5881 tgatacttcc ctttgccaaa tgaaattaac ctctttctct attaccacgt ttttccagaa
     5941 gcaagagatt gggtatcaag aggctggctg ctatgatatg gcgtcttgtt ttttaagcga
     6001 ggaaagattt tgagtgcggg acgcctgaat aaaaaatctc tgggtatcgt gatgttgtta
     6061 tcggttggac tgcttttggc gggctgttcg ggtagcaaat catccgatac aggaacgtat
     6121 tccggctccg tttacaccgt gaaacggggg gatacgctat atcgtatttc gcgcaccacg
     6181 ggaaccagcg taaaagaact ggcgcgactg aacggcattt ccccccctta caccattgaa
     6241 gttggtcaga aactaaaact gggtggggcg aaaagtagca gtattacacg taaatcaacc
     6301 gccaaatcaa cgaccaaaac cgcatcggtt acaccgtcat cagcggtacc gaaatcatcc
     6361 tggccgccag tagggcaacg ttgttggtta tggccaacga cagggaaagt tatcatgccg
     6421 tattcgacag cagatggcgg caataaaggg attgatatct cagctccacg gggtacacct
     6481 atttacgccg cgggtgcagg aaaggtggtg tatgtgggca accaactgcg tggctacggt
     6541 aatctcatca tgattaaaca cagtgaagat tacattacgg cttacgccca taatgacacg
     6601 atgctggtaa ataatgggca aagcgtgaag gctgggcaaa aaatcgccac tatggggagc
     6661 acggatgcgg catctgttcg cctgcatttc cagattcgtt accgtgcaac ggcaattgat
     6721 ccgctacgtt acttgccgcc gcagggcagc aagccaaaat gctgatggcg aattaatcag
     6781 cagtcagcag cgcggcactt gctaaggaga gcgtaaggtt tataatgcct tacgcatctc
     6841 gaagcgggcg tagttcaatg gtagaacgag agcttcccaa gctctatacg agggttcgat
     6901 tcccttcgcc cgctccaatt tatcttcacc tcatcaatct gccatttttg ctgtatctga
     6961 ttttctatat tatcttaatt caatcgcttc agacttttcg caatcgccgt aatatttccc
     7021 cattttgttg taaataaaaa gcgtttacgc aatgcaatcc ttttgtaaca acccaggtaa
     7081 aacaccttat tttcaagttg ttaaataagt ggttatctac gcttttaaca aacgattgat
     7141 ggaatatttt atgaaaggga aatctgcatt aactcttctg ctcgctggta tattttcgtg
     7201 cggcacatgc caggcgacag gggcagaagt gacgagtgaa tctgtattta acattcttaa
     7261 ttccacgggg gcagcaacag ataaaagtta tttatcgtta aatccagata agtaccctaa
     7321 ttatcgtctt ctgattcact cagccaaatt aaaaaatgaa ataaagagcc actataccaa
     7381 agacgaaatt caagggttgt taacattaac agaaaacaca cgaaagttaa cgttgacgga
     7441 gaaaccctgg ggaacattta ttcttgcttc tactttcgaa gatgacaaaa cagcggcaga
     7501 aacccactat gatgctgtct ggctgaggga tagtttatgg ggctatatgg cattagtatc
     7561 agatcaggga aatagtgtag ccgcaaaaaa ggttctgctc accctgtggg attatatgtc
     7621 tacgcccgac cagattaagc ggatgcagga cgtcattagc aaccccaaac gactggatgg
     7681 agttcccggg caaatgaatg ctgtgcatat ccgatttgac agcaactccc ccgtgatggc
     7741 agatgtgcag gaagaaggca agccgcagct gtggaaccat aaacaaaatg atgcgttagg
     7801 tctttatctt gatctcctta tacaagcaat cgacactggt acaattaacg ctgaagattg
     7861 gcaaaaaggt gaccgattaa aatcggttgc gctgctgatt gcttatctgg ataaagccaa
     7921 tttctatgtt atggaagatt ccggagcatg ggaagaagat gcccgattaa acacatcttc
     7981 cgttgcactg gtgacctctg gactggaacg tttgtcgaac ttactctcga agaaagactc
     8041 tgtttttgtc tctgatttac tacgcgaagc taaagccaat gaactggatg aacctctttc
     8101 aacaacgagg ttgaaccacc tcatagataa aggttatgaa agaataacgt tacagcttga
     8161 tttaggggga gaatccccag gttatctgga aaaagataaa cattacagag aagccgatgc
     8221 ggctttactt aatgttattt atcctgcaaa tcttgcaaag ataaacacca gaagaaaaga
     8281 gcaggttcta aaaatagtta aaaaactagc aggtccgtat ggtataaaaa gatacgagaa
     8341 ggataattat cagtcggcaa atttttggtt caatgatatt aaaaccgata ccgatcaaaa
     8401 ttcacatgtg aaaagagaaa agagcttcat tccatcaaca gaagcagaat ggttttttga
     8461 ctcatggtat gcaaaatcag cagcaattgt ttataaagaa tcaagaaaag aagagtattt
     8521 gaatgactca gtgcaattta tgaatagatc attggcccaa ataacaggcg agaatatgat
     8581 tggtgcaaat ggcaggagtg tacctgaaat ggcacttccc gaaagctata actatatcca
     8641 caagtcggga actttgcatg aagcgccaaa ccctatcatt ccgctaaatt ggtcgaaagc
     8701 ttcaatgact ctcatgttga aagagatgtc cagccttatc aatgatgagg gaaataaata
     8761 gttttcataa tgaggcggtc ctggattaaa aggaccgctc aaagagaaat cgaaagggga
     8821 ggtaaaagtg atcaataaat tactgttagc ttacctgata ggtctggttg tgacatcgac
     8881 ttttatattt atcttctcgg aagaaaaagt aacatatcgg ttgtttgcta caataattac
     8941 aggtttaacc tggccgctta gtc
//
LOCUS       DBANUU010000161         8883 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000161 DBANUU010000000
VERSION     DBANUU010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.163"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            76..258
                     /locus_tag="ACIE68_004016"
                     /pseudo
     CDS             76..258
                     /locus_tag="ACIE68_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            424..845
                     /locus_tag="ACIE68_004017"
                     /pseudo
     CDS             424..845
                     /locus_tag="ACIE68_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236032.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1501..2571
                     /locus_tag="ACIE68_004018"
                     /pseudo
     CDS             1501..2571
                     /locus_tag="ACIE68_004018"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            2644..2793
                     /gene="ydfW"
                     /locus_tag="ACIE68_004019"
     CDS             2644..2793
                     /gene="ydfW"
                     /locus_tag="ACIE68_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfW"
                     /protein_id="HFX5910252.1"
                     /translation="MDNARIDLRSKYYVKPKADHPWLTRRTQSHQQVKPPKLPKKKPD
                     PDKKD"
     gene            complement(3230..3562)
                     /gene="flxA"
                     /locus_tag="ACIE68_004020"
     CDS             complement(3230..3562)
                     /gene="flxA"
                     /locus_tag="ACIE68_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFX5910253.1"
                     /translation="MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISS
                     EEGMTTQQKKEMAALVQKQIESLWAQLEQLLRQQAEKKNEDATVQPDKKEEKKDDTNT
                     AGTIDIYV"
     gene            complement(3765..4070)
                     /gene="ydfV"
                     /locus_tag="ACIE68_004021"
     CDS             complement(3765..4070)
                     /gene="ydfV"
                     /locus_tag="ACIE68_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFX5910254.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLVFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSVLLSRRRVSGDLTCR"
     gene            4095..4334
                     /gene="relB"
                     /locus_tag="ACIE68_004022"
     CDS             4095..4334
                     /gene="relB"
                     /locus_tag="ACIE68_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFX5910255.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            4334..4621
                     /gene="relE"
                     /locus_tag="ACIE68_004023"
     CDS             4334..4621
                     /gene="relE"
                     /locus_tag="ACIE68_004023"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFX5910256.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            4693..4848
                     /gene="hokD"
                     /locus_tag="ACIE68_004024"
     CDS             4693..4848
                     /gene="hokD"
                     /locus_tag="ACIE68_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFX5910257.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            5065..5316
                     /gene="rem"
                     /locus_tag="ACIE68_004025"
     CDS             5065..5316
                     /gene="rem"
                     /locus_tag="ACIE68_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFX5910258.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDARVTDGFILREGEFVTSLQALKEIGIKAGFSAFSGE"
     gene            5383..5661
                     /locus_tag="ACIE68_004026"
     CDS             5383..5661
                     /locus_tag="ACIE68_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910259.1"
                     /translation="MARNAKYYNSDNSPVLACTHGRYSHAFKSEWFQHPPCTAEQAEW
                     LIHSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            5663..6712
                     /gene="ydfU"
                     /locus_tag="ACIE68_004027"
     CDS             5663..6712
                     /gene="ydfU"
                     /locus_tag="ACIE68_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFX5910260.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     TETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKSPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEDKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            6726..7478
                     /gene="ydfT"
                     /locus_tag="ACIE68_004028"
     CDS             6726..7478
                     /gene="ydfT"
                     /locus_tag="ACIE68_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFX5910261.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVSFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEASTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(7756..7845)
                     /locus_tag="ACIE68_004029"
     CDS             complement(7756..7845)
                     /locus_tag="ACIE68_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910262.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLAVIALLII"
     gene            complement(7900..8112)
                     /gene="cspF"
                     /locus_tag="ACIE68_004030"
     CDS             complement(7900..8112)
                     /gene="cspF"
                     /locus_tag="ACIE68_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HFX5910263.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            8413..8628
                     /gene="cspB"
                     /locus_tag="ACIE68_004031"
     CDS             8413..8628
                     /gene="cspB"
                     /locus_tag="ACIE68_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HFX5910264.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAANVIITD"
     gene            complement(8618..>8883)
                     /locus_tag="ACIE68_004032"
     CDS             complement(8618..>8883)
                     /locus_tag="ACIE68_004032"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910265.1"
                     /translation="STYALCFAMPAVSFCCKTIHAANLHFTTMVSILFLIKILPNMLA
                     IVSCIGPPYFLLKRSNIHYSVRISTQASAFFVIVYRRAMNFNQ"
BASE COUNT         2457 a         1826 c         2224 g         2376 t
ORIGIN      
        1 aaaatttaat ccttaaccgg atggatttct gcaccctcag aacatcagga ggccgcccga
       61 aagggcggta gtgaaatgcg aaagttcaaa attattattg aaacgggaat agctggtgga
      121 gattttgagg atgtattcga agtggacgat gacgcaacac ctgaggaaat tcatgacgga
      181 gcataagaaa ttttctttaa ctactgcaat tactcatacc acgaaataaa agacgatgag
      241 gaagaacaaa gtggctgatt tttgttcagc taaatataac atcagttttg aagagcggga
      301 tgaactatta atggactatg gtgagttacg cggtggaagt gctgctgatg tcgaatccta
      361 gcgtgatgac tatgaagcgg gaaaaactcc ggtcgaagca tattgtgatg agtggggcga
      421 tgaatgagcg agattaatta tcaggaaggg catgaaacgg cggggcaagc aaaaccagtg
      481 gcatggcgat atcgctacgt gaaaaaaggc gttacggact ttcaggggaa gtagtggtct
      541 ggtgactgga aatatgtacc gaaaaaagag gattgtaacg acaggccgaa ctatcaaatt
      601 caggccttat tcactgcctc accagtccca gttacatcag aagaactggt taaagctgtg
      661 cacttttatg aacaactaaa acgcgaaaat ccaccaacat ccggcaacta gattaatgga
      721 ttaactatgt cggttaaacg accagccaac tgaaaaagcg gaaacctgat tacaggttgc
      781 cagataaggc aatgagctac ctggcgcgga acggactgat aagtatgggg aatgttttac
      841 gatgaacctt tagactaaag agtttgtaac gctatgtaag tgattttttc tggtttagat
      901 atttatatgt ccggccaaat tgaggtgtgt ttaaatgtaa ttgcacattg attgtaggag
      961 gaataatgaa aaacgcattg cagtttttgt ttgttgcgtt ctggttgttc gcatcatgta
     1021 tgcccatcat cttcacagca aggtatatgg aaaaagttga tgttttgata ttaatatttg
     1081 gatatataaa tgcccttttt ttaggggtgt tcatggcggt catgtgcatt gaatactggc
     1141 ggtaaataca gcgaactcca ttggtttagt tggatattta ctgtgctgga caaaaacggt
     1201 ttgcggggaa atcttagtta agtagaatga ctgcgggtgc ttgaggctat ctgcctcggg
     1261 catggacacc aacggcagat agagaaaagc cccagttaac attacgcgtc ctgcaagacg
     1321 cttaacatta atctgaggcc aatttcatgc tagacacatg taggttagcc tcttacgtgc
     1381 cgaaaggcac ggagaagcag gctattgtta acaccaagct gtaatgtccc ctttgaacca
     1441 ttctaaaatg tccccagaca attctctggg ggatttttca tgatcaaaga gactgttacg
     1501 atgagtcata aggaactcca ccgacttcag attattcagg aacaagctgc ggcacgcatt
     1561 ggcatttcta ttcggcaggt taaacgtctg gtgcaacggt atagaaatga agggccttct
     1621 ggtctggttt cccaccgacg tggaaagcgt cctaataatt ccttttctac tgaattcaga
     1681 gcaacagtaa tttcactcct caaaggccgt tacgctgatt ttggacctac gtttgcgtgc
     1741 gaaaaattgc gcgagataca cggtttatct ttatccgttg aaactctcag aaagtggatg
     1801 atagaagagg ggttatggcg tgaacgccgt cgtaaaattg cccgtatata tcaacgccgc
     1861 atgcgacgac catcttacgg tgaactgatc cagattgatg gctcacctca tgactggttt
     1921 gaaaatcgag gccccagatg tacactgatc gttttcattg atgatgccac cagtgcgttg
     1981 atggcgttgc gttttgtgcc tgctgaaaca acccgggctt acatggaaac cctccggggt
     2041 taccttaatg atcatggcgt accgctcgct ctctactctg atagacacag tatattcagg
     2101 gtaaataacc cagagcggga aggtgagctg acccagttca ctcgtgcgat aaagacactg
     2161 ggcatcgagc caatccatgc caacagcccg caggcaaaag ggcgggtaga gcgcgccaat
     2221 cagacactac aggacaggct ggtcaaagaa atgcggcttc agaatatcag tgatattgaa
     2281 acagcaaatg catggttgcc gacctttatt gaagcctata acaaccggtt cgctacgtcg
     2341 cctcgtacta ctgataatgc tcatcttgat gtgcaccatt ctgaagagga actgggttat
     2401 atcttcagcc tacaggcgaa gcgcgttctg tctaaaaatc tcactttcca gtacaaaagc
     2461 agtgcgtttc aggtacgcag tgagggccgg ggatatcgac ttaggcattc ggttgttact
     2521 gtatgcgaga actttgacgg tgaaattaac gttctgtatg acgggaaagc gctgggctgg
     2581 gaaaagtatg ttgatggccc ggagcctata ccactggatg atgaaaagag tgtccatgaa
     2641 cgagtggata atgcccgtat tgatttacgc tcaaaatact atgttaaacc taaagctgac
     2701 catccctggc ttacgcgccg aacgcaaagt catcagcaag ttaagccccc gaagttacct
     2761 aaaaagaagc ctgatcccga taaaaaagat tgaaaccaag atcgattcgg ttgagtgcat
     2821 atccattcat agggtagatt cttaagtcgc gtttctggtg ttcattttcg ggtggtttgt
     2881 tacttgtttt accggggata tgccagaaac gcgctgagtc agtctgggcg gtgcgcgtaa
     2941 tgaggcgtta tggtaaatag cctatgctaa tgtccgctaa gagcaagaag cggaagttgg
     3001 cagttttgtg gactgtcccc acaaaagtga ctacagaaag agttgcaatt cataattgat
     3061 catgggttgt cagttaaact cgtggcgatt taaatagact aattgggagt gcgtccatta
     3121 cttatatctt gtaatgttaa ctatcagaaa tgatacaaag ataatatgtc tttaaagaaa
     3181 aggctgatgg cgaaaagtgg cccgatgagg gccacaatac ggctgtcact tagacgtaaa
     3241 tatcaatggt gccagcggta tttgtatcgt cttttttctc ttctttttta tcaggctgaa
     3301 ctgtcgcgtc ttcattcttt ttctctgcct gctgccttaa caactgctcc agttgagccc
     3361 agaggctttc aatttgcttc tgtaccaatg cagccatttc ttttttctgc tgtgtcgtca
     3421 tcccctcttc cgatgagatt ttcccaagct tttcagtcag cacctgaatt tgtcttgtga
     3481 ttttggctat ttctgatgtt ccttccgggg cggagttgtt tgaaataacg gttgaggtat
     3541 ttccctgaat tgtgacagac atagatttct ccttttaaaa aagcactatc ggcatgcaca
     3601 aaaaaatctt taatcgtatt tcttgtgtca ttaattgttt gatgttcaga ttgttttcct
     3661 cgcgggctgg cgcgcctcag aaagtaaagc ttgttgacag gggtaaacgt tcggcaataa
     3721 ttttctgccg catgcgggtg ttgcataaaa cgtgttacgt tcctttatcg acaggtcagg
     3781 tcaccgctca cccgccgacg agaaagcaac actgacatgc taaagcaaaa aatagatgaa
     3841 taagttgagt tgtgcatatg tagcctgacc gtcacaaagt atatggtgtc tgtaccagta
     3901 agatgatggc cggactcttt aaaaacgagc tgacctgcac aatacaggat ggacttagca
     3961 atggctgctc ctggcacaaa gcggacagtg atcaccgttc ttacgactac tttctgactt
     4021 ccttcgtgac ttgccctaag catgttgtag tgcgatactt gtaatgacat ttgtaattac
     4081 aagaggtgta agacatgggt agcattaacc tgcgtattga cgatgaactt aaagcgcgtt
     4141 cttacgccgc gcttgaaaaa atgggtgtaa ctccttctga agcgcttcgt ctcatgctcg
     4201 agtatatcgc tgacaatgaa cgcttgccgt tcaaacagac actcctgagt gatgaagatg
     4261 ctgaacttgt ggagatagtg aaagaacggc ttcgtaatcc taagccagta cgtgtgacgc
     4321 tggatgaact ctgatggcgt attttctgga ttttgacgag cgggcactaa aggaatggcg
     4381 aaagctgggc tcgacggtac gtgaacagtt gaaaaagaag ctggttgaag tacttgagtc
     4441 accccggatt gaagcaaaca agctccgtgg tatgcctgat tgttacaaga ttaagctccg
     4501 gtcttcaggc tatcgccttg tataccaggt tatagacgag aaagttgtcg ttttcgtgat
     4561 ttctgttggg aaaagagaac gctcggaagt atatagcgag gcggtcaaac gcattctctg
     4621 aaccaaagca tgacatctct gtttcgcacc gaaggtgaca cttctgcttt gcgttgacag
     4681 gagaagcagg ctatgaagca gcaaaaggcg atgttaatcg ccctgatcgt catctgttta
     4741 accgtcatag tgacggcact ggtaacgagg aaagacctct gcgaggtacg aatccgaacc
     4801 ggccagacgg aggtcgctgt cttcacagct tacgaacctg aggagtaaga gacccggcgg
     4861 gggagaaatc cctcgccacc tctgatgtgg caggcatcct caacgcaccc gcacttaacc
     4921 cgcttcggcg ggtttttgtt tttattttca acgcgtttga agttctggac ggtgccggaa
     4981 tagaatcaaa aatacttaag tagcgcgcag ggataagagg gatggtccct taaaggggag
     5041 agctaattat ccggaaggat tctgatgatg aacatcgaag aactgcgtaa aattttttgt
     5101 gaagatggcc tctatgctgt gtgcgttgaa aatggaaatc ttgttagtca ttaccgcatt
     5161 atgtgtttgc gaaagaatgg ggctgcgtta attaattttg tggatgctcg ggtcacggac
     5221 ggatttatct tgcgcgaagg tgagtttgtc acttcattac aggcattgaa agagatcgga
     5281 ataaaagctg gcttttctgc tttttcagga gaataaactc atctacaatc ttgcgcgggg
     5341 ctgaactccc gctgagtaac accgtgccac cggagaaaac cgatggcacg caacgcaaaa
     5401 tattacaatt ctgataattc gcccgttctt gcctgcacgc acgggcggta ttctcacgca
     5461 ttcaagtctg aatggttcca gcaccctcca tgcactgcag aacaggccga atggctgatt
     5521 cattcttacc gcaggcgcgg gttcgaggtt aagaaagctc tcagtctcga ctatcggcac
     5581 tggataatct ctgtcaggct gccttattcc gaacgcccac cacgtgcgtc ccgcactttc
     5641 cagcaacgga tctggaggta acgtgcgggt attacttaga cctgttctgg tgcctgagct
     5701 tgggctggtg gtccttaagc cgggccgtga atccatacag atatttcata atcctcgagt
     5761 gctggtggaa ccggaaccaa aaagcatgcg taatctgcca tccggagtcg ttcctgccgt
     5821 tcgccagccg ctggcggaag acaaaacatt gctgccgttt tttagtaacg aacgggtgat
     5881 tcgtgctgct ggcggcgttg gcgcattgtc cgactggcta ttacgtcatg ttacatcctg
     5941 ccagtggcct aatggcgatt accatcacac tgaaacagtc attcaccgtt atggtaccgg
     6001 cgcaatggtg ttgtgctggc actgcgacaa ccaactgcgt gaccagacat cggaatcact
     6061 ggagctgctt gctcaacaaa atctgacagc atgggtgatt gacgtcatcc gtcacgcaat
     6121 aagcggtacg caggagcggg aattatcttt ggctgaatta tcctggtggg cggtctgcaa
     6181 tcaggtggtg gatgcactac ctgaggctgt atcgcgtcgt tcgctgggat taccagcgga
     6241 aaaaatctgc tcggtgtacc gcgaaagcga catcgtaccg ggagagcaga ccgccaccag
     6301 catattgaaa caacgcacaa aaaatcttgc accgttgcct tacgcccacc agcaacaaaa
     6361 atcaccacag gaaaagacgg tggtaagcat caccgttgat ccagagtctc cggaatcttt
     6421 catgaagctg cctaaacgtc gccgctgggt taaggagaaa tacacacgtt gggttaagac
     6481 acagccgtgt gcttgctgcg gtatgccagc cgacgatccg catcatctga ttggtcacgg
     6541 gcagggcgga atgggaacaa aagcacatga tctctttgtg ttgcctttgt gcagaaagca
     6601 tcacaacgag ctgcatacgg atacagtggc atttgaagat aagtatggct cccaactgga
     6661 gctgatattt cgttttatcg atcgcgcgct ggcaattggc gtactggcgt aagtggagaa
     6721 cgagcatgaa ccttgaagcc ttaccaaaat attactcccc aaaatctcca aaattgagcg
     6781 atgacgctcc agcgacaggc accggttgtt taacaattac ggatgtaatg gcagcgcagg
     6841 ggatggtgca gtcgaaagca ccacttgggt tggccttatt tctggcaaaa gttggtgttc
     6901 aggaccctca gtttgcgatt gaaggcctgc taaattacgc gatggcactg gataacccga
     6961 cattgaacaa attgagtgaa gaaatccggt tacagattat tccttacctc gtgagttttg
     7021 cctttgctga ttactccagg tctgcggcaa gtaaggctcg ctgtgagcat tgttcaggta
     7081 cgggatttta taatgtattg cgcgaagtgg tgaaacacta cagacgcggg gaatctgtaa
     7141 tcaaggaaga atgggtgaag gaactatgtc agcattgcca tggtaagggc gaagccagca
     7201 cagcgtgcag agggtgtaag ggtaaaggga ttgttctgga tgaaaaaaga acccggtttc
     7261 atggcgtacc ggtatataag atttgtgggc gttgtaatgg aaaccggttt agtcgtttac
     7321 cgaccacgct ggcacgacgt catgtccaga agctggtacc agacctgacc gattatcagt
     7381 ggtataaggg gtatgcggac gtcattggta aactggtaac aaagtgctgg caggaagaag
     7441 catacgcgga agcgcaattg aggaaggtga cgagataaat gatttttgct gaagatggcg
     7501 acatgatgtt tgcatttttc aaaaaatatg gataaaattt tttcaacgat gggctttgta
     7561 tacccgacgt taagaaaaag tagaaaaccc gctgatgagc gggttttgtg ctttaaatgg
     7621 ggcaatggta atgttgaatc tcatcccggg actcatgtct gttaacttat tatttagctg
     7681 gtgacttggt tatttgcctg atgtttaaaa tgttttcttc cagtacaatg tccctaaaca
     7741 caatgagtct gcttattata ttattagcag agctattacg gccaaagtac agcataagct
     7801 tttaaagcca atcaaccagt catcaagaca gacggggtta ttcataaaaa ctctccatgt
     7861 gtgatccgat ggggcctgaa attaaagctt taatatagct catgaaaggt aaacattggc
     7921 agctgaaggg ccacgcagac catttatccg gcaaaattcc acgcgtaatc cggtggtaat
     7981 ttcttctgca tcgcggagat tgagcgctga aacatgaagc tggacatcga tacgaccatc
     8041 ggatggggtg ataagaccct tgccgctttt gccgtcaaag gttttgacaa ttcctgtcat
     8101 tttacgggac aaaaaaattc cttaatactg ataacttggc gcactataca cacgttcctg
     8161 aagaaagcta tagttttttg atggggttga agatggctgg atgtctaaaa taaacattgc
     8221 ttcatatgtt caactatgcg ttaatgattg cgtcggtttg aagaacagac gatatacgaa
     8281 gtagtttact aaagcagttc tcatttcagg tgttattcac ttattccttc tttgagtctc
     8341 tccaattaag tacgaagtcg tttctgttat gcaaaccatt tatgccgaaa ggctcaagtt
     8401 aaggaatgta gaatgtcaaa taaaatgact ggtttagtaa aatggtttaa cgctgataaa
     8461 ggtttcggct ttatttctcc tgttgatggt agtaaagatg tgtttgtgca tttttctgcg
     8521 attcagaatg ataattatcg aaccttattt gaaggtcaaa aggttacctt ctctatagag
     8581 agtggtgcta aaggtcctgc agcagcaaat gtcatcatta ctgattaaaa ttcatcgctc
     8641 gtctgtatac gataacgaag aaggctgatg cctgagtaga gatacggaca gagtagtgaa
     8701 tattggatct ctttaataaa aagtaaggag gtccaataca tgaaacaatg gctagcatat
     8761 ttggcaaaat cttaatcagg aaaagtatgc taaccattgt ggtgaagtgc aggtttgctg
     8821 catgaatagt tttacagcag aagctaactg ctggcatggc aaaacaaagt gcgtaagtgg
     8881 atg
//
LOCUS       DBANUU010000162         8845 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000162 DBANUU010000000
VERSION     DBANUU010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.164"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..166)
                     /locus_tag="ACIE68_004033"
     CDS             complement(<1..166)
                     /locus_tag="ACIE68_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFX5910266.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIIS"
     gene            410..667
                     /gene="ynaJ"
                     /locus_tag="ACIE68_004034"
     CDS             410..667
                     /gene="ynaJ"
                     /locus_tag="ACIE68_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFX5910267.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(717..1667)
                     /gene="uspE"
                     /locus_tag="ACIE68_004035"
     CDS             complement(717..1667)
                     /gene="uspE"
                     /locus_tag="ACIE68_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFX5910268.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1819..2571)
                     /gene="fnr"
                     /locus_tag="ACIE68_004036"
     CDS             complement(1819..2571)
                     /gene="fnr"
                     /locus_tag="ACIE68_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFX5910269.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2766..3281)
                     /gene="ogt"
                     /locus_tag="ACIE68_004037"
     CDS             complement(2766..3281)
                     /gene="ogt"
                     /locus_tag="ACIE68_004037"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFX5910270.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3292..4818)
                     /gene="abgT"
                     /locus_tag="ACIE68_004038"
     CDS             complement(3292..4818)
                     /gene="abgT"
                     /locus_tag="ACIE68_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFX5910271.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            complement(4855..6300)
                     /gene="abgB"
                     /locus_tag="ACIE68_004039"
     CDS             complement(4855..6300)
                     /gene="abgB"
                     /locus_tag="ACIE68_004039"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFX5910272.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(6300..7610)
                     /gene="abgA"
                     /locus_tag="ACIE68_004040"
     CDS             complement(6300..7610)
                     /gene="abgA"
                     /locus_tag="ACIE68_004040"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFX5910273.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            7786..8694
                     /gene="abgR"
                     /locus_tag="ACIE68_004041"
     CDS             7786..8694
                     /gene="abgR"
                     /locus_tag="ACIE68_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFX5910274.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
BASE COUNT         2251 a         2215 c         2196 g         2183 t
ORIGIN      
        1 cactgataat tatggtgtag attaaaaatt gcaccgcatg gaataaaaaa ccttttcttt
       61 ttcgatttcc gcggcgaaac caaaagctca tcagaatcaa tgctgcgcag ctaccgaaaa
      121 taatgaccag attaagcgca ttatttgtaa acagttcagc gatcattgtt ttatcaggct
      181 cctccagata attgtcgtca tgccggaaac ccctggcggg gctattttac cgcgacaatt
      241 cattcagatc atcaatagtc agggaaggaa gtagcaacat tagctaacta atcaatgcgt
      301 tgattgtaaa tccagctaag aggtgaggtt ttcagagcag acaacggtga aatgtcatgg
      361 tattgttacg tttaggtaac aagaaatttg tctgcacaag gattacatca tgattatggc
      421 gaaactgaag tcagcgaaag ggaagaaatt tctctttggt ttgttggcgg ttttcattat
      481 tgcggcgtcg gttgtgactc gcgcgactat cggcggcgtt atagaacagt acaatattcc
      541 gctgtctgag tggacgacat caatgtatgt gattcagtca tcgatgattt ttgtttatag
      601 cctggtcttt actgtgttgc tggcaatccc gttgggaatt tatttccttg gcggcgaaga
      661 gcagtaagta aaaaataggc ccgataactc gggccttgtc agttattgaa gagtcgttaa
      721 tcgtcttctt cgtcatccag ttcaacgggt gtctgatact ggtcaggttt aatgaccaac
      781 agatcgcagc gaagatgatc aatcacctgt tccgccgtgt tgccgaggaa tgctgctgaa
      841 ataccggtgc gtcctaccgt gcccagaacc acaatccccg cctgtaagtg ctccgccaaa
      901 tcaggaatca cctcttctgg cagacctttt tctacgtgcg tcatgttttc attaatgccg
      961 aatttctgcc gcagagcttt cattgccagc aaatgttgcc cacgaatggc atcgttataa
     1021 acgctcggat caaattccgg cagttcaatc gcgatattaa ttggcgttac cggataagcg
     1081 ccaaccagat gaacttcggt atggttgact tgttctgcca gttcgatcgt ctctttgacc
     1141 agtttttcat tgagcgcatt atgatacggc tcttcactgg cgagattcac cgccaccagc
     1201 gccttgcctc cttccggcca cggctggtct ttcaccatcc acaccgggct tgggcatttg
     1261 cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt ccagacggtc atgttggtgc
     1321 gccattttta gcaccaaatc gtgcccgccg ctgatcactt cctgaatgat ggcttcgaag
     1381 ggacggttat gccagaccac tttaatttca atgggaacgc cagcattgag ataatatttt
     1441 gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc cctgacgcat agcggtacgt
     1501 tcgtccgggg agagcagggt ggtcatttcg tatgagaagt catagatcgg caaaaaggct
     1561 ttaattttgc caccaatccg ttgatgtaaa taaacagctc gccgcaatgc tggttggtcg
     1621 tcctggttag gatcgataac aacgagcatg ttctgataca tagccataca gggtctcctt
     1681 acaacaactg tcaacgcagt ttgtaattaa aagattaacc catatctggt gaatgaaaca
     1741 gtgatgaacc ttctgccaga tcaataaatc agaaaaattt aatgatatga cagaaggata
     1801 gtgagttatg cggaaaaatc aggcaacgtt acgcgtatga ccagcaagct gggccagcgc
     1861 atcgttattt tcgatggtga tgtatttacc tttgactgcc agcatgccgc ttttctggaa
     1921 gcgacccagc agacggctga tggtttctac cgtcaggccc agatagttac cgatatcgcc
     1981 acgagtcatc gtcaggcgga attcacgagg ggagaagccg cgttgggcaa aacgacggga
     2041 caggttgtag atgaatgcag ccagacgttc ctcggcattt ttcttcgaca acagcaggat
     2101 catgtcctga tcgcctttga tttcaccgct catcagacgc atcatctgct gacgcagatt
     2161 cggcatttta ccagacaaat cgtccagcgt ttcgaacggg atttcacata ccatcgaggt
     2221 ttccagcgcc tgcgcgaagc tcgggtgatg gccgctgccg atggcgtcaa atcccaccag
     2281 gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct tgctcagtga tggtataact
     2341 tttaatcgta ccggagcgga tggcataaag cgatttaagt tcatcaccag ccttaaacag
     2401 cgtctggcct ttctgaatag gcttcttccg ctcaatgata ttatcaagct gatcaagctc
     2461 atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg ctgcaatcct ggcaatggat
     2521 agcacaaccg ccagactgaa tgcgccgtat aattcgcttt tccgggatca taggtctgct
     2581 caagccgtaa ttgatatttg tcaattttaa catcttttta gggagcaagt aagtctaagc
     2641 aaaccttaac agcagagaat tccgatatta gatgtaaata tatgtctatc tatttgaaaa
     2701 cccttaagtt gttaagggta tctttacata aaagtgtgaa caagctggca caaattgttt
     2761 aatgtttaca gcaaaagata accttcgtgg cgcaataacc actcttttcg ctgaactccg
     2821 cctgcatatc cggtcatggt gccgtttcgg ccaataaccc gatggcaagg tacgacgatg
     2881 ctgatgggat tcgatccgtt tgccgcacca acggcacgcg ccgcgccagg gcggcccaat
     2941 tgttcagcca gttggccgta atgcattacc tgcccgcagg ggatagtgcg tagagttttc
     3001 cagacttcgc gctgaaatgg cgtcccccct gtggcagtgg gaagcgtatc aataatgcta
     3061 agattaccgg caaaataatc acgaagcttg tcgcttaaac cgcctgggtt ggtggcagaa
     3121 atgcgctcat agccttcttt gcgataatgg atgtccagca gctgcaccat gcgttcgctg
     3181 tactcttccc attcaaccgc ccgcaggcga aattgctcat cgcaaatcac ccacagtgga
     3241 cccagtggcg tggcaatttt ttcttcaagt aatctcagca tccgttctct cttaagacaa
     3301 acgtgggtaa atccccggac ctatcggcag accaacaaga taccacgcca gcaacatcaa
     3361 cagccatacc accaaaaaga taagcggata gggcaagact aacgaatagt aagtacccag
     3421 tttcgcgtct ggtttgtagc gttgcaggaa tccaagaaat agtggaacaa aaggagatac
     3481 cggcgctaaa ggcaatacgg atgagtcggc aatacgaaag aggatttgcg caaatgccgg
     3541 gtgaaagcca agtagcataa acattggtac gaaaatgggg gccagaatcg accagattgc
     3601 ggaaccgctg gcaataaaca tgcataagaa agaggaaagc aacgccagac cgacaaacgc
     3661 cgggatgccg ctaagccctg aactttcaag tatatcggtc agccccacgg cgatgaattt
     3721 ccccatgttg ctccagttaa acatggcgac aaactgggct aggggaaaaa ccatcacgat
     3781 aaatcccgcc atctctttca tcggttcaat cattaaatgc ggtaaatccg cctgacgtcg
     3841 aattgtgcgg gtagcgatgc cataagccag cgagacaaca aagaaaaaaa gaatgatcag
     3901 tggcacgata cctttaataa agggtgatgg catcacggtg tgattaatcg gatcgcgcaa
     3961 tatcccgttt tccgggatca ccatcagcgc gatcgcagca ataaaaagta gcgatacgac
     4021 acctgctatg cgtaaaccaa aacgctgact ttcggtcaat gtctgcagtt tctcatcgct
     4081 gtttccctgc cattgaccta accgtggctc gatgattttg tcggttatca ggccgccaac
     4141 aatcgtcagt acgactacgg agctggccat aaaataccag ttatcaatta cactgacgtg
     4201 catttgcgga ttgaacgcag ccgccgcttc cgtgctgatc cccgacagca acacgtcggt
     4261 tgtgacaatc agtaaattag ccgtgaagcc gcaacctacg cctgcaatgg cggccagtaa
     4321 acctgcaact ggatgcctgc ccaccgccag aaaaatcagc gcacccatcg gtggcatgat
     4381 cactaacgcc gcatcggaag aaatgtggct gaaaaaagca ataaacagca ccatataact
     4441 ggcgtagcgg gcattaacat gcgatgccat tttaaccatt agcgctggca gtaagccgac
     4501 gcgctccgct aggccggcac ctaaaaccag cgccaggatc gctccaagtg gagcaaaacc
     4561 gctaaagttt ttaatcacat tgggtaaaaa ccagtgtaat ccttctacac tgagcaggtt
     4621 tttcaccacc accggcgtac catcggtcgg gtttttcgca ctgacgccaa aggctgacaa
     4681 aattgccgtc gtcaccatga gtacgattat caaatagata aagagcaaaa aaggatgggg
     4741 aaccttgtta ccaattcttt cgacccagcc atagagcttc ccggattggg aggacgacgg
     4801 tatggatgac atactcatgg gcattcctcg gttgttgtgt ttgcgttgtt gttgttattt
     4861 taaaggtgac ggtgtcacgt ttttcgggat agggcagtga tacggttgcg tgtccgtaac
     4921 ttgctgatgc tcttgttggc attcttgcaa tagccctgaa tcaatgaaga gattgactgt
     4981 ggttgctgcc atagttttcg ccgccagcag cattccttta tgagcaatag atgttcgccc
     5041 ctggctaacc agttgccacg tatgtagcgg tgtaccgacg gcaaaacagg ggctgaaaca
     5101 ctgggcaaca ggcagtttcc aactgacgtc gccgacatca gtcgatgccg caagcacgtt
     5161 atcggtggcg gcatatggag cgacctcatt cgccagtacc gtttcacgat gacgtagtgc
     5221 aaaagccttg ccgttttcgc caccggttgc ggcgatatta ttcagactgt tttgccgatc
     5281 gttgggggtg agcgtagcct gaatttgttt cgcaaaagcc agttcttcgg agttccattc
     5341 cggggtacca aaatgggata gggcatggta catggcattt tctaaggtgc gattcgggag
     5401 ataactggaa caggctttgt cgaagcggca ttcaaccgtg gtttcggtca tcaatgccgc
     5461 accttcggcg attttggcga cccgatcata aatatgctgc acgtcggtca tttcgggggc
     5521 gcggataaga taaagcactt ctgcctgcgc ctggaccacg ttgggcgaga tcccgccgct
     5581 atttgtgatg gcatagtgta cgcgcgcttt ttcaataata tgttcgttga ggaagttggt
     5641 gccagtggtc atcaacgtta cggcatcaag ggcgctgcgt cccaaatgag gggaattcgc
     5701 ggcatgtgct gcgatccctt taaagcacca tgatgcctga atgtttgcca gcgtgcgggt
     5761 attgaacatg ccggcaaagg cttccgggtg ccaggtgagt gccgcatcca catcatcaaa
     5821 taccccctcg cggaccatga acgttttacc cgagccgcct tcttcgccag gacaaccata
     5881 aaagcgcact gtgccgcctt gcccatattg ttccagccat ttcttgacgg ctattgcagc
     5941 ggcaaaggcg gcggttccca gcaaattgtg tccgcaaccg tgaccatttt caccgggcgt
     6001 cgcggatgta ggttgcgcgc aaccagcttg ctgacttaaa cctgccaggg cgtcatattc
     6061 tcccagcagg gcgataaccg gtttgccttg accaaacgaa gcaataaatg catttgggat
     6121 attgcctacg ttgcgggtaa cggtgaagct tgcagattcc agcgccgaag ccagatgctc
     6181 cgctgaccag aactcttcaa aacgtgtttc tggatgatcc cagatttgat cggcaatatc
     6241 ggtataacgt tggcgatcgg cttcaatcgc atcgtcgata aaacgataga tttcctgcat
     6301 cagatacctc gcgtccaggg aaaattgagc gcggtgcgcg ccagcgtttc gacggcaata
     6361 gcgagaacct gctcgtcaaa atcgaatttt tcgttgtgat gacctgccgc cagctgtgtg
     6421 ccaaacacca cgtaggaggc ttgcccttga tgttgctgca cgcgggccat cattaatgtg
     6481 gcatcttcgg aacccgcagg cgcttcaaca cgttcaatgg cctgattgac ccccgcgacc
     6541 tgagccgcct gactttgcaa ccatgcgacc cattgcggcg aaggagaact ggcggtagct
     6601 gcacccatca gacgagtttc aacgccgaca ccatacatgg ttgctgcgcc ctgaatcgct
     6661 tgttgtgcac ggtcaaaaac atattgatta atgacgtcgc tggccccgcg tgtttccact
     6721 ttcagcaacg ccgaggcagg aacaacgtta cggccgcttc ctgcctgcat aacgcccacg
     6781 tttactctgg aagctccttc gctgtgcggg gcgattgcat gcagtgcaag agttgcttgt
     6841 gctgccgcca acaaggcatt gtgaccgtct tctggttttg cgcctgcgtg agcggcggtc
     6901 ccggtgaagt gcgcgtcaaa tttggtggtt gccataaaat tatcactgcc gcacaccacg
     6961 gtgcccgcag gtacgccagt gccaatgtgc acggcagtaa aataatcaac atcatctacg
     7021 acacctgcat cgaccatcgc ccgcgcgcca cgcgtacctt cctctgcagg ctgaaaaatc
     7081 agtttgatga cgccatgtag tccggactcg aattgtttaa gggtatgcgc cagcccaagc
     7141 ccaatggcgg tatgtccatc atgaccacag gcatgcatca ttccggcgtt acatgacgca
     7201 aaaccgtcgc ggtaggggcg atggctgaca tcctgctctt cactgagatc cagcgcgtcc
     7261 atatcgacac ggaaagccat caccggaccg gggcgaccgg tatccagggt ggcgacgata
     7321 ccagtgaaac caccttcaaa aaccgcaatc cattgtgcta gcgcaccctg ttgacgagcg
     7381 cgctcgaatt cgcgttgtag agtgagttca tcaggtagtc ccatccggct actttcatta
     7441 actacttcgc ggcccagcgc cagtgaatag ccgagctgct gcaattcttc cgcaacaagg
     7501 gtggcagtgc ggaattccac ccagccagac tctgcatagt gatgaaaatc acgtcgccag
     7561 tgcgataatt ttggggcaag cgaattaaca aattgattca aagactccat aacctttccc
     7621 gtcatcagta aaaagtgtga cccggttcac gtagcgatag tttttactta tcactaactg
     7681 atttttcaca gttttaaccg ttcataaatt accctgacac aatcatctgc attaaagtag
     7741 atgccagttt ctttggtctg ataaataacg gttatcggtg gcgtcatggc ttttcaggta
     7801 aaaattcatc aaattcgtgc ttttgttgaa gtggctcgtc agggcagcat tcgcggagcg
     7861 agccgaatgt tgaatatgtc gcaaccggca ctgagtaaat ctattcagga gctggaagaa
     7921 ggattagcgg cgcaactctt ttttcgccgt agtaaaggcg tgacgttaac tgatgccggt
     7981 gaaagttttt atcagcacgc cagtcttatt cttgaagagc tgcgcgcagc gcaagaggaa
     8041 attcgccaac gacaagggca actggcaggg cagattaata tcggcatggg ggccagtatt
     8101 tcccgcagtc tgatgccagc tgtcatatct cgttttcatc agcagcatcc gcaggtaaaa
     8161 gtacgcatta tggaagggca actggtgtcg atgattaatg aattgcgtca gggagaattg
     8221 gatttcacca tcaataccta ttatcaggga ccgtacgacc acgaatttac ttttgagaaa
     8281 ttactggaaa agcaattcgc gatcttttgc cgcccgggac accccgccat tggtgcccgt
     8341 tcgatcaaac agttactgga ttacagctgg acaatgccga cgccacacgg cagttactac
     8401 aaacagttga gtgaattgct tgacgatcag gcgcaaacgc cacaggtcgg tgtagtctgc
     8461 gagacgttct cagcctgtat cagtctggtg gcaaaaagcg attttctcag catactgcct
     8521 gaagaaatgg gctgcgatcc cttgcacgga caggggctgg tgatgttgcc ggttagcgaa
     8581 attttaccga aagcggccta ttatttgatt cagcggcgtg atagtcgcca gacaccactg
     8641 accgcgtcat taatcacgca attccggcga gaatgcggct atctgcaaag ttaaaactgc
     8701 ataaaaaaat agagtctgtc gacatccgcc agactctaca gtacacacag cagtgcatcc
     8761 gcgtcttaag tccggcattg tctcctctgc gccggtgact gtgttataac ggttttaacc
     8821 tttagttgcc aattttcttc agtga
//
LOCUS       DBANUU010000163         8714 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000163 DBANUU010000000
VERSION     DBANUU010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.165"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(251..514)
                     /gene="rpsT"
                     /locus_tag="ACIE68_004042"
     CDS             complement(251..514)
                     /gene="rpsT"
                     /locus_tag="ACIE68_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFX5910275.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            617..835
                     /locus_tag="ACIE68_004043"
     CDS             617..835
                     /locus_tag="ACIE68_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFX5910276.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            843..1784
                     /gene="ribF"
                     /locus_tag="ACIE68_004044"
     CDS             843..1784
                     /gene="ribF"
                     /locus_tag="ACIE68_004044"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFX5910277.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIAHDELTAREFFGLTKPA"
     gene            1827..4643
                     /gene="ileS"
                     /locus_tag="ACIE68_004045"
     CDS             1827..4643
                     /gene="ileS"
                     /locus_tag="ACIE68_004045"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFX5910278.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4643..5137
                     /gene="lspA"
                     /locus_tag="ACIE68_004046"
     CDS             4643..5137
                     /gene="lspA"
                     /locus_tag="ACIE68_004046"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFX5910279.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5262..5711
                     /gene="fkpB"
                     /locus_tag="ACIE68_004047"
     CDS             5262..5711
                     /gene="fkpB"
                     /locus_tag="ACIE68_004047"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFX5910280.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5713..6663
                     /gene="ispH"
                     /locus_tag="ACIE68_004048"
                     /gene_synonym="lytB"
     CDS             5713..6663
                     /gene="ispH"
                     /locus_tag="ACIE68_004048"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFX5910281.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6729..7643
                     /gene="rihC"
                     /locus_tag="ACIE68_004049"
     CDS             6729..7643
                     /gene="rihC"
                     /locus_tag="ACIE68_004049"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFX5910282.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7810..8631
                     /gene="dapB"
                     /locus_tag="ACIE68_004050"
     CDS             7810..8631
                     /gene="dapB"
                     /locus_tag="ACIE68_004050"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFX5910283.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
BASE COUNT         1981 a         2168 c         2473 g         2092 t
ORIGIN      
        1 acctcaatgt gtatcacaat atccatattc tttgtggggg agtctggaga ttgagtagat
       61 attcttgttc agaatgtatc agccgatggt tctacgattc ttaagccacg aagagttcag
      121 atagtacaac ggcatgtctc ttttgactat ctggcaaccg gcagtgtgtt ctctcacgca
      181 tcacaaaagc agcaggcata aaaaaacccg cttgcgcggg ctttttcaca aagcttcagc
      241 aaattggcga ttaagccagt ttgttgatct gtgcagtcag gttagcctta tgacgtgcag
      301 ctttgttttt gtggatcaga cctttagcag cctgacggtc cacgatcggt tgcatttcgt
      361 taaatgcttt ctgtgcagca gctttgtcgc cagcttcgat agctgcgtat actttcttga
      421 tgaaagtacg catcatagag cgacggcttg cgttgtgctt acgagccttt tcagactgaa
      481 tggcgcgctt cttagctgat ttgatattag ccaaggtcca actcccaaat gtgttctata
      541 tggacaattc aaaggccgag gaatatgccc ttttagcctt cttttgtcaa tggatttgtg
      601 caaataagcg ccgttaatgt gccggcactc gttacgtagt gatggcgcag gattctacca
      661 gcttgcgggg tgtgaataca gcttttccgc gataaaaatt gcagcaggcg gtcagtttct
      721 tcccgtgatt tgcgccatgg caatgaaaag ccacttcttt ctgatttcgg tactcaatcg
      781 ccggttaacc ttgaccgctg tacaaggtat actcggacga ttttcactgt tttgagccag
      841 acatgaagct gatacgcggc atacataatc tcagccaggc cccgcaagaa gggtgtgtgc
      901 tgactattgg taatttcgac ggcgtgcatc gcggtcatcg cgcgctgtta cagggcttgc
      961 aggaagaagg gcgcaagcgc aacttaccgg tgatggtgat gctttttgaa cctcaaccac
     1021 tggaactgtt tgctaccgat aaagccccgg caagactgac ccggctgcgg gaaaaactgc
     1081 gttaccttgc agagtgtggc gttgattacg tgctgtgcgt gcgtttcgac aggcgttttg
     1141 cggcgttaac cgcgcaaaat ttcatcagcg atcttttggt gaagcatttg cgcgtaaaat
     1201 ttcttgccgt aggtgatgat ttccgctttg gcgctggtcg tgaaggcgat ttcttgttat
     1261 tacagaaagc tggcatggaa tacggcttcg atatcaccag tacgcaaact ttttgcgaag
     1321 gtggtgtgcg tatcagcagc accgccgtgc gtcaggcgct ggctgatgac aatctggctc
     1381 tggcagaaag tttactgggg cacccgtttg ccatctccgg gcgtgtagtc cacggtgatg
     1441 aattagggcg cactataggt ttcccgacgg cgaatgtacc gctacgccgt caggtttccc
     1501 cggtgaaagg ggtttatgcg gtagaagtgc tgggcctcgg tgaaaagccg ttacccggcg
     1561 tggcaaacat cggaacacgc ccaacggttg ccggtattcg ccagcagctg gaagtgcatt
     1621 tgttagatgt tgcaatggac ctttacggtc gccatataca agtagtgctg cgtaaaaaaa
     1681 tacgcaatga gcagcgattt gcgtcgctgg acgaactgaa agcgcagatt gcgcatgatg
     1741 aattaaccgc ccgcgaattt tttgggctaa caaaaccggc ttaagcctgt tatgtaatca
     1801 aaccgaaaca cggaaccgag aatctgatga gtgactataa atcaaccctg aatttgccgg
     1861 aaacagggtt cccgatgcgt ggcgatctcg ccaagcgcga accgggaatg ctggcgcgtt
     1921 ggactgatga tgatctgtac ggcatcatcc gtgcggctaa aaaaggcaaa aaaaccttca
     1981 ttctgcatga tggccctcct tatgcgaatg gcagcattca tattggtcac tcggttaaca
     2041 agattctgaa agacattatc gtgaagtcca aagggctttc cggttatgac tcgccgtatg
     2101 tgcctggctg ggactgccac ggtctgccga tcgagctgaa agtagagcaa gaatacggta
     2161 agccgggtga gaaattcacc gccgccgagt tccgcgccaa gtgccgcgaa tacgcggcga
     2221 cccaggttga cggtcaacgc aaagacttta tccgtctggg cgtgctgggc gactggtcgc
     2281 acccgtatct gactatggac ttcaaaactg aagccaacat catccgcgcg ctgggcaaaa
     2341 tcatcggcaa tggtcacctg cacaaaggcg cgaagccggt gcactggtgc gtagactgcc
     2401 gttctgcact ggcagaagcg gaagttgagt attacgacaa aacttctccg tccattgacg
     2461 tcgctttcca ggcggtcgat caggatgcgc tgaaagcgaa atttggcgta agcaacgtta
     2521 acggcccaat ctcgctggtg atctggacca ctacgccgtg gactctgcct gcgaaccgcg
     2581 caatctctat tgcacctgat ttcgactatg cgctggtgca gatcgacggt caggccgtga
     2641 ttctggcgaa agatctggtt gaaagcgtaa tgcagcgtat cggcgtgacc gattacacca
     2701 ttctcggcac ggtaaaaggt gcagagcttg agctgctgcg ctttacccat ccgtttatgg
     2761 gcttcgacgt tccggcaatc ctcggcgatc acgttaccct ggatgccggt accggtgccg
     2821 ttcacaccgc gcctggccac ggcccggacg actatgtgat cggtcagaaa tacggcctgg
     2881 aaaccgctaa cccggttggc ccggacggca cttatctgcc gggcacttat ccgacgctgg
     2941 atggcgtgaa cgtcttcaaa gcgaacgaca tcgtcgttgc gctgctgcag gaaaaaggcg
     3001 cgctgctgca cgttgagaaa atgcagcaca gctatccgtg ctgctggcgt cacaaaacgc
     3061 cgatcatctt ccgcgcgaca ccgcagtggt tcgtcagcat ggatcagaaa ggtctgcgtg
     3121 cgcagtcact gaaagagatc aaaggcgtgc agtggatccc ggactggggc caggcgcgta
     3181 tcgagtcgat ggttgctaac cgtcctgact ggtgtatctc ccgtcagcgc acctggggcg
     3241 taccgatgtc actgttcgtg cacaaagaca cggaagagct gcatccgcgt accctcgaac
     3301 tgatggaaga agtggctaaa cgcgttgaag ttgacggcat ccaggcgtgg tgggatctcg
     3361 atgcgaaaga gatcctcggc gacgaagctg accagtatgt gaaagtgccg gatactctcg
     3421 acgtatggtt tgactccgga tctacccact cttctgttgt tgacgtgcgt ccggaatttg
     3481 ccggtcacgc agcggacatg tatctggaag gttctgacca gcaccgtggt tggttcatgt
     3541 cttccctaat gatctccacc gcgatgaaag gcaaagcgcc gtatcgtcag gtactgaccc
     3601 acggctttac cgtggatggt cagggtcgca agatgtctaa atccatcggc aacaccgttt
     3661 cgccgcagga tgtgatgaac aaactgggcg cggatattct gcgtctgtgg gtggcatcaa
     3721 ccgactacac cggtgaaatg gccgtttctg acgagatcct gaaacgtgct gccgatagct
     3781 atcgtcgtat ccgtaacacc gcgcgcttcc tgctggcaaa cctgaatggt tttgatccag
     3841 caaaagatat ggtgaaaccg gaagagatgg tggtactgga tcgctgggcc gtaggttgtg
     3901 cgaaagcggc acaggaagac atcctcaagg cgtacgaagc atacgatttc cacgaagtgg
     3961 tacagcgtct gatgcgcttc tgctccgttg agatgggttc cttctacctc gacatcatca
     4021 aagaccgtca gtacaccgcc aaagcggaca gtgtggcgcg tcgtagctgc cagactgcgc
     4081 tgtatcacat cgcagaagcg ctggtgcgct ggatggcacc aatcctctcc ttcaccgctg
     4141 atgaagtgtg gggctacctg ccgggcgaac gtgaaaaata cgtcttcacc ggtgagtggt
     4201 acgaaggcct gtttggcctg gcagacagtg aagcgatgaa cgatgcgttc tgggacgagc
     4261 tgttgaaagt gcgtggcgaa gtgaacaaag tcattgagca agcgcgtgcc gacaagaaag
     4321 tgggtggctc gctggaagcg gcagtaacct tgtatgcaga accggaactg tcggcgaaac
     4381 tgaccgcgct gggcgatgaa ttacgatttg tcctgttgac ctccggcgct accgttgcag
     4441 actataacga cgcacctgct gatgctcagc agagcgaagt actcaaaggg ctgaaagtcg
     4501 cgttgagtaa agccgaaggt gagaagtgcc cacgctgctg gcactacacc caggatgtcg
     4561 gcaaggtggc ggaacacgca gaaatctgcg gccgctgtgt cagcaacgtc gccggtgacg
     4621 gtgaaaaacg taagtttgcc tgatgagtca atcgatctgt tcaacagggc tacgctggct
     4681 gtggctggtg gtagtcgtgc tgattatcga tctgggcagc aaatacctga tcctccagaa
     4741 ctttgctctg ggggatacgg tcccgctgtt cccgtcgctt aatctgcatt atgcgcgtaa
     4801 ctatggcgcg gcgtttagtt tccttgccga tagcggcggt tggcagcgtt ggttctttgc
     4861 cggtattgcg attggtatta gcgtgatcct ggcagtgatg atgtatcgct cgaaggccac
     4921 gcagaagcta aacaatatcg cttacgcgct gattattggc ggcgcgctgg gcaacctgtt
     4981 cgaccgcctg tggcacggct tcgttgtcga tatgatcgac ttctacgtcg gcgactggca
     5041 cttcgccacc ttcaaccttg ccgatactgc catttgtgtc ggtgcggcac tgattgtgct
     5101 ggaaggtttt ttaccttcta aagcgaaaaa acaataataa accctgccgg atgcgatgct
     5161 gacgcatctt atccggccta cagattgctg cgaaatcgta ggccggataa ggcgtttacg
     5221 ccgcatccgg caaaaatcct taaatataag agcaaacctg catgtctgaa tctgtacaga
     5281 gcaatagcgc cgtcctggtg cacttcacgc taaaactcga cgatggcacc accgccgagt
     5341 ctacccgcaa caacggtaaa ccggcgctgt tccgcctggg tgatgcttct ctttctgaag
     5401 ggctggagca acacctgttg gggctgaaag tgggcgataa aaccaccttc tcgttggagc
     5461 cagatgcggc gtttggcgtg ccgtcaccgg acctgattca gtacttctcc cgccgtgaat
     5521 ttatggatgc aggcgagcca gaaattggcg caatcatgct ttttaccgca atggatggca
     5581 gtgagatgcc tggcgtgatc cgcgaaatta acggcgactc cattaccgtt gatttcaacc
     5641 atccgctggc cgggcagacc gttcattttg atattgaagt gctggaaatc gatccggcac
     5701 tggaggcgta acatgcagat cctgttggcc aacccgcgtg gtttttgtgc cggggtagac
     5761 cgcgctatca gcattgttga aaacgcgctg gccatttacg gcgcaccgat atatgtccgt
     5821 cacgaagtgg tacataaccg ctatgtggtc gatagcttgc gtgagcgtgg ggctatcttt
     5881 attgagcaga ttagcgaagt accggacggc gcgatcctga ttttctccgc acacggtgtt
     5941 tctcaggcgg tacgtaacga agcaaaaagc cgcgatttga cggtgtttga tgccacctgt
     6001 ccgctggtga ccaaagtgca tatggaagtc gcccgcgcca gtcgccgtgg cgaagaatct
     6061 attctcatcg gccacgccgg gcatccggaa gtggaaggga caatgggtca gtacagcaac
     6121 ccggaagggg gaatgtatct ggtcgaatcg ccagacgatg tgtggaaact gacggttaaa
     6181 aacgaagaga agctctcctt tatgacccaa accacgctgt cggtggatga cacgtctgat
     6241 gtgatcgacg cgctgcgtaa acgcttcccg aaaattgtcg gtccgcgcaa agatgacatc
     6301 tgctacgcca cgactaaccg tcaggaagcg gtacgcgccc tggcagaaca ggcggaagtt
     6361 gtgttggtgg tcggttcgaa aaactcctcc aactccaacc gtctggcgga gctggcccag
     6421 cgtatgggca aacacgcgtt tttgattgac gatgcgaaag atatccagga agagtgggtg
     6481 aaagaggtta aatgcgtcgg cgtgactgcg ggcgcatcgg ctccggatat tctggtgcag
     6541 aatgtggtgg cacgtttgca gcagttgggt ggtggtgaag ccattccgct ggaaggccgt
     6601 gaagaaaata ttgttttcga agtgccgaaa gagctgcgtg tcgatattcg tgaagtcgat
     6661 taagtcatta gcagcctaag ttatgcgaaa atgccggtct tgttaccggc attttttatg
     6721 gagaaaacat gcgtttacct atcttcctcg atactgaccc cggcattgac gatgccgtcg
     6781 ccattgccgc cgcgattttt gcccccgaac tcgacctgca actgatgacc accgtcgcgg
     6841 gtaatgtctc ggttgagaaa actacccgca atgccctgca actgctgcat ttctggaatg
     6901 cggagattcc gctcgcccag ggagccgctg tgccgctggt acgcgcaccg cgtgatgcgg
     6961 catctgtgca cggcgaatcg ggaatggctg gctacgactt tgttgagcac aaccgaaagc
     7021 cgctcgggat accagcgttt ctggcgattc gggatgccct gatgcgtgca ccagagcccg
     7081 ttaccctggt ggccatcggc ccgttaacca atattgcgct gttactttca caatgcccgg
     7141 aatgtaaacc gtatattcgt cgtctggtga tcatgggcgg ttctgctggt cgcggcaact
     7201 gcacaccaaa cgccgagttt aatatcgctg ccgatccaga agcggctgcc tgtgtcttcc
     7261 gtagcggcat cgaaatcgtc atgtgcggtc tggatgtcac caatcaggca atattaaccc
     7321 ctgactatct cgctactttg ccggagttga atcgcaccgg aaaaatgctc cacgccctgt
     7381 ttagccacta ccgtagcggc agtatgcaaa gtggactgcg tatgcacgat ctttgcgcta
     7441 ttgcctggct ggtgcgcccg gaattgttca ctctcaaacc gtgttttgtg gcagtggaaa
     7501 ctcagggcga atttacctca ggcacgacgg tggttgatat cgacggttgc ctgggcaaac
     7561 cagccaatgt aaaggtggca ttggatctgg atgtgaaagg cttccagcag tgggtggctg
     7621 aggtgctggc tctggcgtcg taacctgtca catgttatcg gcatgcagtc attcatcgac
     7681 tcatgccttt cactgatatc cctccctgtt tatcattaat ttctaattat cagcgttttt
     7741 ggctggcggc gtagcgatgc gctggttact ctgaaaacgg tctatgcaaa ttaacaaaag
     7801 agaatagcta tgcatgatgc aaacatccgc gttgccatcg cgggagccgg ggggcgtatg
     7861 ggccgccagt tgattcaggc ggcgctggca ttagaaggcg tgcagctggg cgcggcgctg
     7921 gagcgcgaag gatcttcttt actgggcagc gacgccggtg agctggctgg tgcagggaaa
     7981 acaggcgtta ccgtgcaaag cagcctcgat gcggtaaaag atgattttga tgtgtttatc
     8041 gattttaccc gtccggaagg tacgctgaac catctcgctt tttgtcgcca gcatggcaaa
     8101 gggatggtga tcggcactac ggggtttgac gaagccggta aacaggcgat tcgtgacgcc
     8161 gccgccgata ttgcgattgt ctttgccgcc aattttagcg ttggcgttaa cgtcatgctt
     8221 aagctgctgg agaaagcagc caaagtgatg ggtgactaca ccgatatcga aattattgaa
     8281 gcacatcata gacataaagt tgatgcgccg tcaggcaccg cactggcaat gggagaggcg
     8341 atcgcccacg cccttgataa agatctgaaa gattgcgcgg tctacagtcg tgaaggccac
     8401 accggtgaac gtgtgcctgg caccattggt tttgccaccg tgcgtgcagg tgacatcgtt
     8461 ggcgaacata ccgcgatgtt tgccgatatt ggcgagcgtc tggagatcac ccataaggcg
     8521 tccagccgca tgacatttgc taacggcgcg gtaagatcgg ctttgtggtt gagtggtaag
     8581 gaaagtggtc tttttgatat gcgagatgtg cttgatctca atagtttgta accacaaaat
     8641 atttgttgtg gtgcaaaaat aacacattta atttattgat tataaagggc tttaattttt
     8701 ggccctttta tttt
//
LOCUS       DBANUU010000164         8622 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000164 DBANUU010000000
VERSION     DBANUU010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.166"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..2668
                     /gene="cas3"
                     /locus_tag="ACIE68_004051"
     CDS             <1..2668
                     /gene="cas3"
                     /locus_tag="ACIE68_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFX5910284.1"
                     /translation="KNIVTFFDFWGKTRRGEKEGGDDYHLLCWHSLDVAAMGYLMVKR
                     NCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPELKIQEGARKN
                     YEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGRPPDRMD
                     ELDNFLPEDKAAARDFLLAIKALFPRIEIPAFWDDDEGIELIKHLSWYISATVVLADW
                     TGSSTRFFPRVAQAMDIKHYWQKALVQAQNALTVFPPKAETAPFTGINTLFPFIENPT
                     PLQQKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGLFFGLPTMAT
                     ANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFS
                     QGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACD
                     AYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGAEGQQEAPLLGKDDY
                     PWLTHVTKTDVHSHRVATRKEVERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSV
                     DDAIKVYRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKEDGSQRAGKVLI
                     CTQVLEQSVDCDLDEMISDLAPIDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELL
                     ILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPHSARLLIESV
                     YGEDVVMPEGFARSEQEQVGKYYCDRARAKKFVLNFRPGYAANINDYLPEKLSTRLAE
                     ESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGDAFRQWCIE
                     QRQDPEMANVILVTDDESCGYSAMEGLTGKVG"
     gene            2766..4328
                     /gene="casA"
                     /locus_tag="ACIE68_004052"
                     /gene_synonym="cse1"
     CDS             2766..4328
                     /gene="casA"
                     /locus_tag="ACIE68_004052"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFX5910285.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDHRRWDDIWEDGLEVEKLREALLSLEHAFQFGPDSPSFM
                     QDFEALTGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKMWLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQNDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRFVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVNLERIMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            4325..4861
                     /gene="casB"
                     /locus_tag="ACIE68_004053"
                     /gene_synonym="cse2"
     CDS             4325..4861
                     /gene="casB"
                     /locus_tag="ACIE68_004053"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFX5910286.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            4873..5928
                     /gene="cas7e"
                     /locus_tag="ACIE68_004054"
     CDS             4873..5928
                     /gene="cas7e"
                     /locus_tag="ACIE68_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFX5910287.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWAMAEKGTEQPRSLAAAFYEPINGTRQLEVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            5939..6685
                     /gene="cas5e"
                     /locus_tag="ACIE68_004055"
     CDS             5939..6685
                     /gene="cas5e"
                     /locus_tag="ACIE68_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFX5910288.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTIQMPKEVRKARYFSRREELSDPD
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            6667..7317
                     /gene="cas6e"
                     /locus_tag="ACIE68_004056"
     CDS             6667..7317
                     /gene="cas6e"
                     /locus_tag="ACIE68_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFX5910289.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELRTGQQLCFNLRANPTICKS
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            7314..8237
                     /gene="cas1e"
                     /locus_tag="ACIE68_004057"
     CDS             7314..8237
                     /gene="cas1e"
                     /locus_tag="ACIE68_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFX5910290.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            8234..8527
                     /gene="cas2e"
                     /locus_tag="ACIE68_004058"
     CDS             8234..8527
                     /gene="cas2e"
                     /locus_tag="ACIE68_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFX5910291.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
BASE COUNT         2139 a         2029 c         2384 g         2070 t
ORIGIN      
        1 taaaaatatt gtgactttct ttgatttctg gggaaaaacc cgacgcggcg agaaagaagg
       61 gggcgacgac tatcaccttc tttgctggca ttcgctggat gtggccgcga tgggctattt
      121 aatggttaaa agaaattgct tcgggctggc tgattatttt cgtcaattag ggatttctga
      181 caaggaacag gcggctcaat ttttcgcctg gttgctgtgc tggcacgata ttggaaaatt
      241 tgcccgctct tttcaacaac tttacctgcc ccctgaactc aagattcagg aaggtgccag
      301 aaaaaattac gaaaaaatct ctcattcaac gctgggctac tggttgtgga atcattattt
      361 aagtgaatgt caggagttac ttccttcatc ttcgctctct ccgcgcaaac ttaggcgagt
      421 gatagaaatg tggatgcccg tgacgacagg gcatcatggt cggccccctg atcgtatgga
      481 tgaattggat aattttctgc ctgaagacaa agctgcagca cgagattttc tccttgcaat
      541 caaggcactg tttccgcgaa tagagatccc cgcattctgg gatgatgacg agggcattga
      601 acttataaaa cacctttcct ggtatatctc tgcaaccgtc gtactcgcag actggacagg
      661 ttcgtcaacg cgattttttc cacgcgtcgc acaggcaatg gatattaaac attactggca
      721 gaaggcttta gtgcaggctc aaaacgcatt aaccgtcttt cctccaaaag cagaaaccgc
      781 acctttcacc ggaattaata cactgtttcc ttttattgag aatccgacac cgttacagca
      841 aaaggtgctg gatctggata tcagccagcc aggaccgcag ttatttattc tggaagatgt
      901 gactggcgca ggtaaaacag aagcggcgct tatcctggcg cacaggttga tagctgcggg
      961 gaaagctcag gggctgtttt ttggtctgcc aacaatggca acggccaatg ccatgtacga
     1021 tcggctggtc aaaacctggc ttgctttcta ttcgccagaa tcccgcccca gcctggtgct
     1081 ggcacatagt gcccgcacat taatggaccg ctttaatgaa tcactctggt ccggtgattt
     1141 agtcgggtca gaagaaccgg atgaacaaac gttcagtcag ggatgtgcgg cctggtttgc
     1201 cgacagtaac aagaaggcgt tgctggctga aattggcgtc ggcacgctgg atcaggcgat
     1261 gatggcggtg atgccgttca aacataataa tctgcggctt ctggggctga gtaacaaaat
     1321 cctgctggct gatgagatcc acgcctgtga cgcttacatg tcgtgcattc ttgaagggct
     1381 gatcgagcgg caggcgcgtg gcggaaacag cgttattttg ctttctgcta cgttatctca
     1441 acagcagcgc gacaaactcg tcgccgcctt tgcgcgtggt gcagagggcc agcaagaagc
     1501 cccactcctg ggaaaagatg attacccctg gctgacgcat gtcacgaaaa ccgatgtaca
     1561 ttcgcaccgg gtagcgacgc gcaaagaagt tgagcgtagc gtgagcgtgg gttggcttca
     1621 tagcgaacaa gaatgtattg cgcgtatcga atcagcggta agtcagggga aatgcatcgc
     1681 ctggatccgg aattctgtcg atgacgctat taaggtttat cgtcagttac ttgcccgcgg
     1741 cgtcattcct gcttccagcc tttcactttt tcatagccgc tttgctttta gcgatcgcca
     1801 gcgaattgaa acggaaacgc tggcacgctt tggtaaagaa gacggttcac agcgtgccgg
     1861 aaaagtcctc atttgtactc aggtcttaga gcagagcgtt gattgtgacc tggacgaaat
     1921 gatctccgac ctggccccca ttgatttgct aatccagcga gcaggacgat tacagcggca
     1981 tatccgcgat attaatggtc agttaaagcg tgacggaaaa gacgagcgtt ccccccctga
     2041 attgctgatt ctggctcccg tctgggacga ctctcctggc gacgaatggt ttggcagtgc
     2101 catgcgtaac agtgcatttg tctatcccga tcatgggcga atctggctga cgcagcgtgt
     2161 gctgcgtgag caaggtgcta ttcagatgcc acactcagcc cggcttctta ttgaatccgt
     2221 ctacggtgag gacgtggtaa tgccggaagg atttgcccgc agcgagcagg agcaagtggg
     2281 caaatattac tgtgatcgcg caagggctaa aaagtttgtg ctgaacttca ggcccggcta
     2341 tgccgccaat atcaacgatt acctcccgga aaaactgtcg acacgtctgg ctgaggagtc
     2401 tgtttccctg tggctggcta cctgtattga cggtgtggtg aagccttatg ccaccggtgc
     2461 tcacgcatgg gaaatgagcg ttgtcagagt gcgccgaagc tggtggaaaa aacatcggga
     2521 tgagttttct ttactggaag gggatgcgtt caggcagtgg tgcattgaac agcggcaaga
     2581 tccggagatg gcaaacgtga ttttagtcac cgatgacgaa agttgcgggt attcggctat
     2641 ggaaggattg actggcaagg ttggttgaga tgcctgcgac agggctctct gaatagttga
     2701 tacaccgaaa aggatggaat gatggacaac cgacctggga tggtcttttt tacgagagga
     2761 tgcgtatgaa ctcgttttca cttctgacaa ccccgtggtt gcctgttcgt ttcaaagatg
     2821 gaacgacagg caaactggcg cctgtcgatc tggcggatga aaatgttgtc gatattgctg
     2881 cgccacgggc agatctccag ggagcggcat ggcagttttt gctggggcta ctacaaacca
     2941 gctttgcgcc aaaagatcat cgtcgttggg atgatatctg ggaagacgga ctggaagttg
     3001 aaaagctacg ggaagcattg ctgtccttag aacacgcttt ccagtttggg ccagattccc
     3061 cttcatttat gcaggatttt gaggcgctca cgggagataa agttcaggtc gcttcactac
     3121 tgcctgagat tcccggcgct caaacaacga agtttaataa agaccacttt atcaagcgtg
     3181 gtgtaacgga atacctgtgc ccacattgtt ctgcgttagc tctgttctcc ctacagttaa
     3241 atgcgccgtc aggtggtaaa ggctaccgca ccggcttacg cggcggtggg ccgatgacca
     3301 cgctgattga attacaggaa tatcagggca atcaacaaac tcccttgtgg cgcaaaatgt
     3361 ggctcaacgt gatgccgcag gatgaagccg atttaccgct acccaaaaaa tttgacgatc
     3421 tggttttccc ctggcttggc ccgacgcgca ccagcgaact ggccggtgcg gtggtaaccg
     3481 atgatcaggt taataaactc caggcgtact ggggaatgcc gcggcgtatt cgtattgatt
     3541 ttaataccac gacagtcggc aactgcgata tttgcggcga gcagaacgac gcgctcctga
     3601 gcctgatgac gaccaaaaat tacggtgcga attatgccat gtggcagcat cccttaacgc
     3661 cttaccgtgt accacttaaa gagggtggcg agttttactc cgttaaacca caaccgggcg
     3721 gtttaatctg gcgtgactgg ttaggcctta tcgaaacggg taaatcagaa aacaatacgg
     3781 aacttcccgc gctggtggtg aaactcttta atgccagcag tctgaaacag gcaaaagtgg
     3841 gcctgtgggg atttggttat gatttcgaca acatgaaagc gcgctgttgg tacgaacacc
     3901 atttccctct attgctcaat aaaaaagaag gccagatacc aaagctgcgg ctggctgcgc
     3961 aaacggcttc acggattcta agtctgttac ggagtgcatt gaaagaagca tggttctctg
     4021 atccaaaagg tgcaaggggt gattttagtt ttgtggatat cgacttctgg aacaaaactc
     4081 agcatcgctt tctgaggttc gtgcgccaaa ttgaagaagg tcaggatgcg gatgaattac
     4141 tcagcaaatg gaataaggaa atttggttat tcgcgcgtca ggattttgac gagcgtgtat
     4201 tcactaatcc ttatgagccc gttaatttgg aacgcatcat gaccgcgcgc aagaaatatt
     4261 ttacaacatc ggcggagaag caaagtgcta aagccgccag ggagaaaaag caggaggctg
     4321 ctgaatgagt attgtaaaag aggagcacaa agccacgctg cgcaaatggc acgaggaatt
     4381 gcaggagaaa cgcggcgaac gtgccagtct gcggcgtagt acgacggtta atgatgtttg
     4441 tctgacggat ggatttcgcc tgtttctgaa gaacagacaa ataaagtggc aggatgaacc
     4501 tgagtggcgt atcaccgcac tagcgctgat agccgcagtt agcgcgaatg ttaaagccat
     4561 tgatgaacgg cagccttttg ccgcgcagct ggcggcggta atgtcaaagg ggcgttttac
     4621 tcgcctgtcg gcagtaaaaa caccggatga attactgcgc cagttacggc gcgcagtcag
     4681 attattaaac ggttccgtca atcttgactc tctggcagag ggggtcttcc gctggtgcca
     4741 ggagagcgac gacctgctca atcatcaccg ccgccagcag cggccaaccg aatttatccg
     4801 tatccgttgg gcgctggaat attaccaggc tggcgacgcc gataacgaac aaaatcaata
     4861 aaggactatc tcatgacaac atttattcag cttcatttgt taaccgctta ccctgctgcc
     4921 aaccttaacc gtgatgatac tggtgcgcca aaaactgtgg tactgggtgg cgcaacgcgt
     4981 ctgcgcgttt cctcacaaag tctgaaacgt gcgtggcgca cttctgcact ttttgaacag
     5041 gcactggcgg gccatattgg tattcgtagt gggcgtattg cgcgtgaggc ggcaactatc
     5101 ctgattgaga aaggaatcga agagaaaaaa gccatcgaat gggcggcaaa aattgcggat
     5161 tatcttggga aagctaaaaa cgacaaaaaa ccaaaagatc cgctcactaa cgccgaaact
     5221 gaacaattag ttcatatcag cccggcagaa tttgacgccg taaaagcgct ggcccatcag
     5281 cttgcagaag aaaagcgcgc gccaaaagag gaagatctcg cgttgttacg caaagaccgt
     5341 atggcggtag atattgccat gtttggtcgt atgctggcga ataaacccga gtttaatgtt
     5401 gaagccgcct gccaggtagc gcacgcattt ggtgtcagtg aaacgattgt cgaagatgat
     5461 tttttcaccg ccgttgatga tttgcgccag gcttctgaag atgccggtgc cgggcatctt
     5521 ggtgaaaccg gattcggttc tgcactgttc tacacctata tctgcattga taaagatctg
     5581 ctggtcgaaa acctcggcgg tgacgaagcg ttagctaatc agaccatacg cgcctttacg
     5641 gaagccgcgc ttaaagtctc cccaaccggc aaacagaaca gctttgccag ccgtgcctac
     5701 gcctcctggg cgatggcaga aaaaggtacc gaacaaccgc gttctctggc ggcggctttc
     5761 tatgaaccca ttaatggcac ccggcagtta gaggtggcgg tgcagcgtat tacaacgctt
     5821 cgcgaaaaca tgaatacggt ctacgaacag aagaccgaat gcgcaagttt tgacgtgatg
     5881 aacaaacagg gaagcatgaa ggacgtgctg gactttatct gcgcgtaagg ggcggcttat
     5941 gagccaatat ctgatttttc agcttcatgg gccaatggca tcctggggcg tcgatgcccc
     6001 cggcgaagtg cgtcataccc atgaactgcc ttcgcgctca gcattgctgg ggctgctggc
     6061 tgccggggta gggattcggc gtgatgatac cgaacgctta aacgcgttta atcgccacta
     6121 ttcactggtg gtttgcgcca gccgtaaccc gcgctgggca cgggattatc acactatcca
     6181 gatgccaaaa gaggtgcgta aagcgcgtta tttcagccgt cgcgaagagt tgagcgaccc
     6241 tgatcttctg agcgcgatta tctcccggcg cgactactac accgatgcct ggtggatggt
     6301 ggcggtggca acaacagccg atgcgcctta cagcctcgaa cagttgcagg acggtttacg
     6361 ccatccagtt tttccgcttt atctggggcg aaaaagtcat ccgctggcat tacctcttgc
     6421 gccgttactg ctcgaaggca acgcgtgtga tgccttatgt aatgcatacc aacagtatca
     6481 ggatcatttc cataaactga aagtctcact cccgaaactt caggatgaat gctggtggga
     6541 aggggaacac gacggcctgg tcgcgagcaa aatattacgc cgtcgggatg ttcctttaaa
     6601 tcgtcagcag tggttgtttg gggaacgcac catcaatcag gggccgtggc tcagcaagga
     6661 ggaaccatgt acctctcaag aataacctta cataccggcc agctttcgcc tgcgcagttg
     6721 ctgcatctgg tggatcgcgg tgaatatgtg atgcatcagt ggctgtggga tcttttccct
     6781 ggcggcaaag aaaggcaatt tctttatcgt cgggaagaac tccagggcgc gtttcgcttt
     6841 tttgtcctct cgcaggaacg cccggcggaa agtgatacgt tcaccataga atgccggtca
     6901 tttgcgcccg agctgcgtac cggacaacaa ctttgtttca acctgcgggc gaatccaaca
     6961 atctgtaaat cgggcaagcg ccacgatctg ctgatggagg cgaaacggca ggtgagggga
     7021 caggcggaag gaagtgatgt ctggttgcat caacaacagg cggcgctgga ctggctggca
     7081 gcgcaggggg aaagaagtgg ttttacacta ctggatacct cggttgatgc ctaccgacaa
     7141 cagcaactcc ggcgggaaaa ctcccggcaa ctgatccagt tcagtagtgt ggattatacg
     7201 gggatgctta cggtcaccga tcccgggtta tttctgcaac gtcttagcca gggatatggc
     7261 aaaagccggg cgttcggctg tggtctgatg ctgatcaaac ccggagcaga ggcgtgacgt
     7321 ttgtaccact gagtccgatc ccgttaaaag atcgcacctc catgatcttc ctccagtacg
     7381 gtcagatcga cgtactggac ggcgctttcg tgctgatcga caaaaccggg atccgcacgc
     7441 acattccggt gggatcggtc gcctgcatta tgctcgaacc gggaacgaga gtttcccacg
     7501 cggcggtgca tctagccgcc acggtgggga cgctactggt ctgggtcggt gaagcgggcg
     7561 ttcgcgttta ctcctccgga caacccggag gggcgcgggc agataaatta ctctaccagg
     7621 caaagctggc tttaacggaa gatctacggt tgaaggtggt gcgcaaaatg tatgaattac
     7681 gttttcgtga accaccgcca gctcgccgtt cagtggagca gttacgtgga attgagggat
     7741 ctcgcgttcg ccagacgtat gcattactgg cgaaacaata tggtgtgaaa tggaatggtc
     7801 gcaaatacga tcctaaagac tgggaaaaag gcgatgttgt gaatcgctgc atcagtgctg
     7861 ctacatcatg tctgtacggt atatctgaag cggcagtatt agccgcggga tatgcgcccg
     7921 ctattgggtt tatccatagt ggcaaaccgc tttcatttgt ttatgacata gccgatatca
     7981 ttaaatttga ttcggttgtg ccaaaggcat ttgaaatagc agcgaggcaa cccgcagaac
     8041 ctgataaaga agtcagatta gcctgtcgcg atattttccg tagcactaag ttaacgggca
     8101 aattaatacc gttaattgag gaagtccttg ctgcaggtga aattgaacca ccacaacccg
     8161 cgccggatat gttaccgcct gccatccctg aacctgaaac gctgggtgat agtggtcacc
     8221 gggggcgcgg cggatgagca tggtcgtggt tgttacagaa aatgtccctc cgcgcttacg
     8281 tggacggctc gcaatctggc tactggaagt gcgtgccggt gtgtatgttg gtgatacatc
     8341 aaaacgtatt cgggagatga tctggcagca aattacccaa ctggctggtt gcggaaatgt
     8401 ggtgatggcc tgggcgacca ataccgagtc gggttttgaa tttcagacct ggggagaaaa
     8461 cagacgtatt ccggtggatt tggatgggtt acgtttggtt tcttttcttc ctgttgataa
     8521 tcaataagtt agacgttctt taaaaataag gaaatgtttt aatttagttg gtagattgtt
     8581 gatgcggaat aaatttgttt aaaaacagtt atgtatgctt ag
//
LOCUS       DBANUU010000165         8430 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000165 DBANUU010000000
VERSION     DBANUU010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.167"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            31..399
                     /gene="cbeA"
                     /locus_tag="ACIE68_004059"
     CDS             31..399
                     /gene="cbeA"
                     /locus_tag="ACIE68_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFX5910292.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            488..862
                     /gene="cbtA"
                     /locus_tag="ACIE68_004060"
     CDS             488..862
                     /gene="cbtA"
                     /locus_tag="ACIE68_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
                     /protein_id="HFX5910293.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            859..1053
                     /gene="yeeW"
                     /locus_tag="ACIE68_004061"
     CDS             859..1053
                     /gene="yeeW"
                     /locus_tag="ACIE68_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFX5910294.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            1099..1179
                     /locus_tag="ACIE68_004062"
                     /pseudo
     CDS             1099..1179
                     /locus_tag="ACIE68_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1468..1596)
                     /locus_tag="ACIE68_004063"
                     /pseudo
     CDS             complement(1468..1596)
                     /locus_tag="ACIE68_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1668..1850
                     /locus_tag="ACIE68_004064"
     CDS             1668..1850
                     /locus_tag="ACIE68_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFX5910295.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(1951..2280)
                     /gene="tmaR"
                     /locus_tag="ACIE68_004065"
     CDS             complement(1951..2280)
                     /gene="tmaR"
                     /locus_tag="ACIE68_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFX5910296.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(2452..3510)
                     /gene="yeeA"
                     /locus_tag="ACIE68_004066"
     CDS             complement(2452..3510)
                     /gene="yeeA"
                     /locus_tag="ACIE68_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFX5910297.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(3708..4181)
                     /gene="sbmC"
                     /locus_tag="ACIE68_004067"
     CDS             complement(3708..4181)
                     /gene="sbmC"
                     /locus_tag="ACIE68_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFX5910298.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(4300..5466)
                     /gene="dacD"
                     /locus_tag="ACIE68_004068"
     CDS             complement(4300..5466)
                     /gene="dacD"
                     /locus_tag="ACIE68_004068"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFX5910299.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQHRLIAVIMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            5675..7102
                     /gene="sbcB"
                     /locus_tag="ACIE68_004069"
     CDS             5675..7102
                     /gene="sbcB"
                     /locus_tag="ACIE68_004069"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFX5910300.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFNVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFEYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLNNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(7145..7372)
                     /gene="tsuB"
                     /locus_tag="ACIE68_004070"
     CDS             complement(7145..7372)
                     /gene="tsuB"
                     /locus_tag="ACIE68_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFX5910301.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(7386..>8430)
                     /gene="tsuA"
                     /locus_tag="ACIE68_004071"
     CDS             complement(7386..>8430)
                     /gene="tsuA"
                     /locus_tag="ACIE68_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFX5910302.1"
                     /translation="LSGLICGALLGFVMQRGRFCLTGGFRDMYLAKNNRMFYALLIAI
                     SVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRAGEGLI
                     GSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVLLVITL
                     WVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGR
                     MFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRATDAQT
                     TLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASWLVYVR
                     PQRKARLATAAAN"
BASE COUNT         2236 a         2186 c         1967 g         2041 t
ORIGIN      
        1 tcttagccga ttttctgtaa ggattttatc gtgtcagaca cactccccgg gacaacactt
       61 cccgacgaca atcacgaccg cccctggtgg gggctgccct gcaccgtgac gccctgtttc
      121 ggggcacgtc tggtgcagga gggtaaccgg ttgcattacc ttgccgaccg cgccggtatc
      181 agaggcctgt tcagcgatgc agatgcgtac cacctggacc aggcctttcc gctgctgatg
      241 aaacaactgg aactcatgct caccagcggt gaactgaatc cccgccatca gcataccgtc
      301 acgctgtatg caaaagggct gacctgcaaa gccgataccc tcagcagttg tggttacgtt
      361 tatctggctg tttatccgac gcccgaaatg aaaaattaac tctccagaat agccttctgc
      421 tacggcctgg tgttttcacc acgccacttt tccattttta tatctgcata tcaggaaaat
      481 cttcagtatg aaaacattac ctgtattacc cgggcaggcg gccagttctc gcccgtctcc
      541 tgttgaaatc tggcagatac tgctgtcccg actgctggac cagcactatg gcctcacact
      601 gaatgacaca ccttttgccg atgaacgtgt gattgagcag catattgagg caggcatttc
      661 actgtgtgat gcggtgaact ttctcgtgga aaaatacgcg ctggtgcgta ccgaccagcc
      721 gggattcagc gcctgtaccc gctctcagtt aataaacagt attgatatcc tccgggcacg
      781 ccgggcaacc ggcctgatga cccgcgacaa ttacagaacg gtaaataaca ttacccttgg
      841 taagtatccg gaggcgaaat gatgacactg gaagccgaca gcgttaacgt acaggcgctg
      901 gatatggggc acattgtcgt tgacattgat ggtgttaata tcactgaact gattaataag
      961 gccgctgaaa acggttattc actccgcgtg gtggatgacc gtgactctac cgaaacaccg
     1021 gcaacttatg ccagccctca ccagttgctg taagacaatg cagtgataca gggtatagtg
     1081 ccattaacca cagaaacagc actgttgtcc tgtattaagc aggcaagaga agatgtcctt
     1141 accctgcgcc atctgcagct tctgcaccag aaccggtgaa tctcactcaa caggcttcat
     1201 ttctcctgac gccagcctga atacagctgg cgttttcatt tataaacaga aaggaaaacc
     1261 gataataatg gaatggacgc cccgaccatg aagcaacggt aggatattac tgataaacct
     1321 tccagtggag gtgcgttatg gccgttttaa cgattagcat tgacctgaca aaaaatgaat
     1381 tccaaatcca cggtctgggt aggaacagaa aaatctaagc tcagaaaacg gattaaagta
     1441 agcgtcaacg gagcaccgta ttgacgctta tttattggtg agaactacgt tccatggcag
     1501 gagttcgtca acacggttgg agggccattc cggcagtatg ctcagaatat ggcgcagata
     1561 cgcttccgga tcgataccgt tcagacggca ggtgcctgct tttctggcaa caagcaggaa
     1621 tctcagcgct gaagcaggaa ttagaggtaa aaacgcccta ccgggccatg aatcacccgg
     1681 tcattggagt agtaaccaaa gcagatttag ccagcatgga acaaatatct ttggtgaaaa
     1741 gttggttacg ggaagctgga gcgcacaacg tgttagtaac cagtgcagtt aataacaatg
     1801 gagttacaga actttttgcc ttgctgcata cagaagaagg ctgttgttaa tttcgattgg
     1861 ttctcggatg agaatgatga cggtgataca cagataagaa tcaatgagca atgagggttg
     1921 ccgggcaacc ctcattgaat aaaacgggaa ttacttcgct tcgccgtttt tcatttcgcc
     1981 catagctttc agctttttgg agatatcgcg gcgttctttg gagagctcgg catttttgat
     2041 gatgtaatcg tcaacgcgat cttcatagtc acctttcatg ctggcgatga tgccctggat
     2101 tgcttcaacg ctcatccccg gcttgatgta atcgctcagg ttgtccagca gcaggacgcg
     2161 cttctggttg tcacggatct ttttctcaac gtcctgaatt tcacgttgca gtttgttctt
     2221 acgacggaac agacgaacaa attccagtac gtcctggaat gaaggcttgg tagtttccat
     2281 ttttataccc ctgataatgt gagagtcgga ttcgtttaag caaccgctat tcgttagggc
     2341 caacattact catagctgcc gcagatgaca atgcttttat cccttttcac tatcataccc
     2401 tttatccttg ctgaatcgaa gcagcagcaa gatgattctg aagttcagga attatttgcg
     2461 caaggcccgg caaatcagat tcgatagcaa ctcaagctga tgcgccgttt ccatgttcag
     2521 ccacacataa ccatagattg gtgtttccac aaccttcagg tcatggtggt tattgagcaa
     2581 ctgacgcagc tcttccacag catcgttcaa tttttccgta ttggcaaaaa ccggctgcgg
     2641 attaccttcg tacagcgcat gaacaaggct cagcagtatt tgctgcatca tgtgctgggt
     2701 atcacgaagt ttttgcgcgt tcaataacac gaaatggctg gggcgcgtgg cccagtatgc
     2761 attgatttgc aactccagca tacaaaccag attgcggtta atggtctgga taccttcata
     2821 tatcgatttt ggaatacggg tttctttgct ggcgggcgca atcagtccac gcattttcac
     2881 ggcatcggtc aggagttttt gtagatggct ttccagacgt gggcgttcga gtaagttcgg
     2941 tgagaatgca gattgataga cccgattata ctcggtcaga cttttcgcca gttgaatgcg
     3001 ccagtggatg aacgcccgtt gtggccagat accggtaaac aacattgcca gtaaagagcc
     3061 gaggatcaca tcgccgcttc gccataacgc cgtatcaatt tcacctgtcg gggaacccac
     3121 aacaattgcc agcgtcaccc caatcaataa accttgatac ggtttcttgc ccagcgccag
     3181 ccaaccgcaa aggaacatgg ccgccgcgca ccagactaac atcagcggta acgagattaa
     3241 ctccagttgc agagcgataa gacctaaaat cgaacccaac accgtaccgc caatacgctc
     3301 aaaggcgcga gggacaacgt taccccagaa cgagattggc cccataatca ccaccatggt
     3361 gaccagcggc caggtgcttt ccgggatagt aaacaggcgg ataatgagaa aagtgagcag
     3421 gaatgccagc gcgacccgag taccatgcac aatgcggtaa tggcggtata cccggatttc
     3481 aaacgggctt aatgacttat cggcacgcac gcagaaactc cgtaatgaga ttaaaagcgc
     3541 tcataactca tctgtcggcc agaaggcgcg cctctgaatt atccgacgga caaaccggga
     3601 acagtgtaat ttcccggcag gagcgaaaaa attgaaaggc gcatcttact cttttcgctt
     3661 tcaaaaaaaa gagtggtcat cgcgttaaca caccgccctg agatgaatta gtgatgtttt
     3721 ggctgcaccg caacatacat ttcgatatcc cagtacccat cttccgcgcc attgttcaaa
     3781 taaacctcga agcagggctt tggtaacatt tcataagcac tgtcctgcaa gaggctatta
     3841 aagaactgat accagggttt agcaaaatca tcaccgacta cacgagctac cgccaccgca
     3901 tactgaccac ctgaaatttc tgtcagaatg acgccctcac tgttttcggg gagcgtaaag
     3961 taacccggca ccgtcacgac ggtgtcgcag cgtaattttt cggcgggtgt ttcatctgga
     4021 ttgtcgtaat agacagcaac ccattccttc ggcacaatat ttttgctatc tacccacatc
     4081 atcaactgct caaaaccttt ctttaccgtc tgttcccacg ggccaacgag atggaaacct
     4141 gcaacggtac gtttatcttc ctgcttaatc tcgtagttca tgacgcctcc attaatactg
     4201 tttttatata cagtatagtt gcaatattaa aaccacaagg aatgagtgtt gattatgcga
     4261 gcagactcgc actcctgcca gtctgctgca aaagaaaggt caggccttat ggtggaaata
     4321 atcactcagg cgagaaaaca tgctgccttc cccgacagat tccagggtaa ccagcggcca
     4381 gtgcgccacc tgtttatcac ggtcgtaaag ttcaatttcc cctacccgct gatgggcgct
     4441 aattggcgcg gtgagttctt taccatcaag ggtatatttg gctttgatat gtggaatttc
     4501 ggctttcggt agcaccatcc agaactcttg ttccgttccc aggtcgatat tttctttatc
     4561 gccataccag atgcgttccg ttccgacctt tttcccacgg tgcaaaattt gcaccgtagt
     4621 aaagttttgt tgcccccaac gcagtaattt tcttgcctct tcctcacgac cttttgcact
     4681 gtcagccccc attatcactg caatgagacg atgctgccca tctacagccg aagcaatgag
     4741 attaaacccg gcaccagaag tatgacccgt tttcaggccg tcaacattca tggttttatc
     4801 ccacaataat ccgttacggt tttgctgggt gataccgttc caggtgagac ttttctcact
     4861 gtacatatga taaaactcgg gctcgccgtg gatgatagcg cgagaaagca cagctaaatc
     4921 ataagccgag ctatgctggc caggtgcatc cagaccatgc actgtttcaa aatgcgtatc
     4981 cttgagatgc agcttctcgg catagttgtt catcatttca acaaactgcc gttgcccacc
     5041 ggcaatatag tcagccagag caacacaagc gtcatttccg gaatccacaa ttaaaccacg
     5101 gcttaaatca cgtaccgata cgcgatcgcc ctctttcaaa aacatcagtg aagaaccgac
     5161 aaacaccgga ttatctttcg cccacgcatc gcgccctacg gtgacaatat cgtctggcgt
     5221 aatgcgatga ctatcgatag cgcgatccac gacatagccc gtcatcagct ttgtcaggct
     5281 ggcgggattg cgctgttgat gctcattacc cgcggtgagg atctgtccgg tggtgtaatc
     5341 catcagtacc caggacccgg catgaatctc tggaggctga ggtgaaaaag gaatgttttc
     5401 cgccgcaaaa ccagacgata agttaaaaac gaacaaagaa gcagcaataa taagacggcg
     5461 tttcaacagc aaaccctcag gagtttcaaa tagctgttct ttttacggaa atacttatga
     5521 actggctgga ataaagtgca agaaaatgtg actaccctct catttttatc tgacatgatc
     5581 tgttgccact agctgccaaa ttgtggcgct aaagctgatt agcacggtga tatttgatac
     5641 tctggcagat agcagaaata acggatttaa cctaatgatg aatgacggta agcaacaatc
     5701 tacctttttg tttcacgatt acgaaacctt tggcacgcac cccgcgttag atcgccctgc
     5761 acagttcgca gccattcgca ccgatagcga attcaatgtc atcggcgaac ccgaagtctt
     5821 ttactgcaag cccgcggatg actatttgcc ccagcctgga gcagtattaa ttaccggtat
     5881 taccccgcag gaagcacggg cgaaaggaga aaacgaagcc gcgtttgccg cccgtattca
     5941 ctcgcttttt aacgtaccga agacctgtat tctgggctac aacaatgtgc gtttcgacga
     6001 cgaagtcaca cgcaacgttt tttatcgtaa tttctacgat ccttacgcct ggagctggca
     6061 gcatgataac tcgcgctggg atttactgga tgttatgcgt gcctgttatg ccctgcgccc
     6121 ggaaggaata aactggcctg aaaatgatga cggtctaccg agctttcgcc ttgagcattt
     6181 aaccaaagcg aatggaattg aacatagcaa cgcccacgat gcgatggctg atgtgtacgc
     6241 cactattgcg atggcgaaac tggtaaaaac gcgtcagcca cgcctgtttg aatatctctt
     6301 tacccatcgt aataaacaca aactgatggc gttgattgat gttccgcaga tgaaacccct
     6361 ggtgcacgtt tccggaatgt ttggggcatg gcgcggcaat accagctggg tggcaccgct
     6421 ggcgtggcat cctgaaaatc gcaatgccgt aattatggtg gatttggcag gagacatttc
     6481 gccattactg gaactggata gcgacacatt gcgcgagcgt ttatataccg caaaaaccga
     6541 tcttggcgat aacgccgccg ttccggttaa gctggtgcat atcaataaat gtccggtgct
     6601 ggcccaggcg aatacgctac gcccggaaga tgccgaccga ctgggaatta atcgtcagca
     6661 ttgcctcaat aacctgaaaa ttctgcgtga aaatccgcaa gtgcgcgaaa aagtggtggc
     6721 gatattcgcg gaagccgaac cgtttacgcc ttcagataac gtggatgcac agctttataa
     6781 cggctttttc agtgacgccg atcgtgcagc aatgaaaatt gtgctggaaa ccgagccgcg
     6841 taatttaccg gcactggata tcacttttgt cgataaacgg attgaaaagc tgttgttcaa
     6901 ttatcgggcg cgcaacttcc cggggacgct ggattatgcc gagcagcaac gctggctgga
     6961 gcaccgccgc caggtcttca cgccagagtt tttacagggt tatgcagatg aattgcagat
     7021 gctggcacaa caatatgccg atgacaaaga gaaagtggcg ctgttaaaag cgctttggca
     7081 gtacgcggaa gagatcgtct aatttgaagc ctccgccgct ggtacggcgg aggacaatgt
     7141 tgagtcaggc tttttgaacg gtgatgctcc acgctgcatc gccaatctgc tgataatcgg
     7201 tgatggcatg tccttcttct gccgcccact gtgggattgc ttcagttgcc tgggtgcaat
     7261 caaactcaat taccaactca tcgccgctgg ccatttctgc cagtgctgct tttgcttcta
     7321 tgagtggaaa cggacagacc tgcgtcacca catccagctt tttaatcgcc atctttacac
     7381 cttacttaat ttgccgcagc agttgccagt cgcgccttac gctgcggtcg aacatacaca
     7441 agccaggacg cagtccagac tccgagaatc ataaatacaa ggccaatcca gccctgccag
     7501 gtcatcattg cagtcataac cagtccatta ccgatagagc aaccacctgc aatactggca
     7561 ccgaagccca tcagtacacc acctaacccg ctacgtaatg ttgtttgtgc atcagttgcg
     7621 cgaacgcgga actcacggct cgctttggct gcaataaatg accccacgaa gatccctaac
     7681 accaggaaaa cgccccagtt aatgtatttc acgtcacccg cgaccagaaa ttgcagaata
     7741 ttggccgttg gggaagtgat tcctaaccca aacatgcgtc cggttgcttc actcaggggc
     7801 caggctaaaa gcgcaatcaa accgatgagt acagcagtga caaagggatg ccagcgtttt
     7861 tcaaacagaa tatgagcgat cccggttcgg cgcggcggta aggtcgcgac tttgagtttt
     7921 ggcttcttca actctttcat caccacccag agcgttatca ccagcaaaac ggcaaccaac
     7981 ggccacacag acaaattaaa agtctcagca atagagttat gttcagtact gtagtgctgc
     8041 aaggtttgat ttaaaccact ggcatgtgga gaacgcatca ccgcactcat caccatataa
     8101 gtgaaaagcg cgatccaact gccgatcaat ccctcacccg cgcgatacca ggtcccggtg
     8161 gcacatccac ccgccagaac aattcccagc ccaaagatat acccacctat aacagtacca
     8221 agccacggga acgccccggc ttcgtaagtc agtaggcccg cctgaattaa cgcaaaaacc
     8281 cccacacttt gtaccgaaat tgcaatcagc aaggcgtaaa acatgcgatt atttttcgcg
     8341 agatacatat cgcgaaaacc acctgtcagg caaaaacgcc cacgctgcat gacaaatccc
     8401 agcagagcac cacaaattag cccgcttaat
//
LOCUS       DBANUU010000166         8374 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000166 DBANUU010000000
VERSION     DBANUU010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.168"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..988)
                     /locus_tag="ACIE68_004072"
     CDS             complement(<1..988)
                     /locus_tag="ACIE68_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFX5910303.1"
                     /translation="MSKYNELVKKLKEIFQIDRPELDFGIYRILNARADEINDYLDNK
                     LKAKIQSALADAGNANKSELEHQLQLTIKAATDAGVDPADSPKVQELKKQLAAMASGA
                     NEHENAVFSHLLTFFSRYYDNGDFISKRRYKGNTYAIPYSGEEVMLHWANKDQYYIKS
                     GENFANYSFKLEDGRKVSFKLLAADTAKDNRKDNELDRCFVLIEPHVRTKIDEEGDEY
                     EQEYKPVEVVKNSSVVDGKLVETEELVIHFEYKAMKKGTKQDALVQSAISTILADKTV
                     QQHWVDLAKRAPTEKNPSRTELERHLTTYTQRNTADYFIHKDLGGFLTNELDF"
     gene            complement(1009..3900)
                     /locus_tag="ACIE68_004073"
     CDS             complement(1009..3900)
                     /locus_tag="ACIE68_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFX5910304.1"
                     /translation="MIGTTPYHAKYFAHELSILHSNNGVDRLSQSLFDASVDLNPHQI
                     EAALFAIENPLSKGVVLADEVGLGKTIEAGLVLCQLWAERKRKLLIICPASLRRQWAS
                     ELQEKFNLPSQVLDAKMYSQLQKEGIHNPLNNKSITIMSYHYAARLEEKLVAEMWDLV
                     VIDEAHKLRNAHRESNKMGQALKRALDGRKKLLLTATPLQNSLMELYGMSTLIDEHTF
                     GEVKAFRKQYMQADSDIAELKGRLSRFIKRTLRKNVLEYIKYTERKAITIPFYPSEQE
                     QDLYERVQKLLEREDSYALPKRHRHLTGLILRKLLSSSTKAVLNNLQILKTRLERLKL
                     EGIVEDDMDIIQQIIMDDDLEDDIVEDAESVAPDTECKSVDSDALQAEINELESLIVK
                     AEQIGTDTKSKELLSGLEQGFAQLAEMGAAKKVIIFTESMRTQQYLAHFLENNGYQGK
                     VVTFSGTNNTPQANKIYQQWREEYQGSSRITGSAQIDKRSALIDHFKDHAEIMIATEA
                     AAEGVNLQFCSLLINYDLPWNPQRIEQRIGRCHRYGQKFDVVVINFLNQRNQADQRVL
                     ELLTEKFSLFDGVFGASDSVLGSIENGVDFEKRIQSIYESCRTPEEIELAFERLQKEL
                     EDEINLKMQKTQEQLLEHFDEDIHDILKIRLDEARERLDKVGRWFWAVTCQQLKQYAD
                     FDHEHHRFTLRESVNELPVGQYQLLRKDGRQNSALQMQDHHSFAYRINHPLGEYVIDG
                     AKAQETKTAEVIFDYSNHTTKVSVAEQLKGQSGWLTLNLLTVDAFNRQQFLVFTAKTD
                     NGRVVDGEACKRLFNLSAITNEKLDNSASLDALPDDLILLKNRQIDARLAEVLEQNNV
                     LFEAERDKLEKWAEDMIFAAEEALRDTKMQIKSLKREARLAQSIEEQKQNQEKLKLLE
                     RQQKRQRMEIFDIEDEIADKRDELISALEERMKQKTDITELFTIRWKVI"
     gene            complement(4451..4585)
                     /locus_tag="ACIE68_004074"
                     /pseudo
     CDS             complement(4451..4585)
                     /locus_tag="ACIE68_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(4555..4704)
                     /locus_tag="ACIE68_004075"
                     /pseudo
     CDS             complement(4555..4704)
                     /locus_tag="ACIE68_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4810..4986)
                     /locus_tag="ACIE68_004076"
     CDS             complement(4810..4986)
                     /locus_tag="ACIE68_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFX5910305.1"
                     /translation="MKSLTTETALDILITWLQDNIDCESGIIFDNDEDKTGSAALLRC
                     IEQAREGIRTLRQQ"
     gene            complement(5003..5494)
                     /locus_tag="ACIE68_004077"
     CDS             complement(5003..5494)
                     /locus_tag="ACIE68_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFX5910306.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDIDGVNLAELINMVCDNGY
                     SLRVVDESDRTSADCTPPFVALTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGTGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAGYLPGLPT
                     FDW"
     gene            complement(5491..5865)
                     /locus_tag="ACIE68_004078"
     CDS             complement(5491..5865)
                     /locus_tag="ACIE68_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFX5910307.1"
                     /translation="MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGASSQLINSIDILRARRATG
                     LMTRHNYRTVNNITLGKYPEAK"
     gene            complement(5955..6323)
                     /locus_tag="ACIE68_004079"
     CDS             complement(5955..6323)
                     /locus_tag="ACIE68_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFX5910308.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
     gene            complement(6486..6707)
                     /locus_tag="ACIE68_004080"
     CDS             complement(6486..6707)
                     /locus_tag="ACIE68_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFX5910309.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(6770..7246)
                     /locus_tag="ACIE68_004081"
     CDS             complement(6770..7246)
                     /locus_tag="ACIE68_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFX5910310.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(7262..7747)
                     /locus_tag="ACIE68_004082"
     CDS             complement(7262..7747)
                     /locus_tag="ACIE68_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFX5910311.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(7802..>8374)
                     /locus_tag="ACIE68_004083"
     CDS             complement(7802..>8374)
                     /locus_tag="ACIE68_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFX5910312.1"
                     /translation="MLRLRRAGEINGEHVPEIILLNSHDGTSSYQMLPGYFRFVCQNG
                     CVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFDRIEEKRDAMQSLILPPPARQALAQ
                     AALTYRYGDEHQPVTTADILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRI
                     HTRAIHSIDTDIKLNRALWVMAETLLESLR"
BASE COUNT         2060 a         2064 c         1947 g         2303 t
ORIGIN      
        1 aaaaatccag ttcgttcgtt aaaaaaccac cgagatcttt atggataaag tagtctgctg
       61 tattacgctg ggtataagtt gtaaggtgac gctccaactc tgtacgtgac ggattctttt
      121 ctgttggcgc acgtttagca agatcaaccc aatgctgttg aactgtttta tctgccagaa
      181 ttgttgaaat ggcggactgt actaaagcat cctgcttagt gccctttttc attgccttat
      241 attcgaagtg aataactaac tcttctgttt caacgagttt accgtccacc acagatgagt
      301 ttttcaccac ttctactggc ttatattctt gttcgtactc atcgccttct tcatcaattt
      361 tggtacgaac atgtggctca ataagtacaa agcagcgatc taattcatta tctttacggt
      421 tatcttttgc tgtgtccgct gcgagcaatt taaagctaac tttacgaccg tcttctaatt
      481 tgaacgaata attagcgaag ttttcaccgc ttttaatgta gtactggtcc ttatttgccc
      541 agtgcaacat cacttcttcg ccagaatagg ggatggcgta ggtattacct ttataacggc
      601 gtttgctgat aaaatcgccg ttgtcataat aacgcgaaaa gaaagtgagc agatgggaaa
      661 atactgcatt ttcatgctca tttgctccgg atgccatagc cgccagttgc tttttaagct
      721 cctgtacttt tgggctatca gcagggtcta cacccgcatc agttgccgct tttattgtca
      781 gttgcaactg atgttctaat tctgatttat tggcatttcc tgcatccgcc agcgcagact
      841 ggattttggc cttcagctta ttatccagat agtcgttaat ttcatcagca cgcgcattta
      901 aaatacgata aataccgaaa tccagttctg gtcgatcaat ctgaaaaatt tcttttagct
      961 tcttaactaa ttcgttgtac ttactcatta ttttctcttt caaatcaatt aaataacttt
     1021 ccagcggatg gtaaacagct cggtgatatc cgtcttttgc ttcatccgct cttcaagggc
     1081 actgatgagt tcatcccgct tgtcggcgat ctcatcttca atgtcaaaaa tttccatacg
     1141 ttggcgcttc tgctgccgtt ccaggagctt aagcttttcc tggttttgct tttgttcttc
     1201 gatcgattgc gccagcctgg cttctcgctt caacgacttg atttgcattt tggtatcgcg
     1261 taatgcttct tccgccgcaa agatcatgtc ttcagcccat ttttccagct tatctctttc
     1321 cgcttcaaaa aggacattgt tctgctccaa aacttctgca agcctggcgt caatttgcct
     1381 gttcttgagt aagatcaagt catcaggtaa tgcatctaaa cttgccgaat tatccagctt
     1441 ttcgttggtt attgcagaca agttgaacag acgtttacaa gcttcaccat ctaccacacg
     1501 tccgttgtca gttttcgcgg taaagactaa aaattgctgc ctgttaaacg catcaacagt
     1561 aagcaaattt agtgttaacc atcctgattg ccctttcagc tgctcagcca ccgaaacttt
     1621 cgttgtgtgg ttactgtaat cgaagatgac ttcagctgtt ttcgtctcct gggctttagc
     1681 tccgtcaatg acgtactcac ccagtggatg attgatccga tatgcaaaac tgtggtgatc
     1741 ctgcatttgt aacgcactat tttgcctgcc atctttgcgc aacagctggt actgcccgac
     1801 aggcagttca ttgactgatt cacgtaaagt gaaacgatga tgctcatgat caaaatcagc
     1861 atattgtttg agctgctgac aggtcacggc ccaaaaccaa cgaccgactt tatccaggcg
     1921 ctcccgagct tcatcaagcc ttattttaag gatatcgtga atatcttcat caaaatgctc
     1981 aagcaactgt tcttgcgtct tctgcatctt gagattgatt tcatcttcca gctctttctg
     2041 caacctttca aaagccagct ctatctcctc gggggtacgg catgactcat aaattgactg
     2101 gatccttttt tcaaaatcca caccattttc tatgctgcca agcacactat ccgatgcgcc
     2161 aaacacgcca tcaaacaggc tgaacttttc agttaagagt tcaagaacgc gttgatcggc
     2221 ctggtttcgc tgattgagga agttaataac aaccacatca aatttttgcc cgtatcggtg
     2281 acaacgtcca atacgctgct caatgcgttg tgggttccag ggtaaatcgt aattgatcag
     2341 tagcgaacaa aattgcaggt tcaccccttc agctgcagct tctgtagcta tcatgatttc
     2401 agcatgatct ttgaagtggt cgatcaaagc cgagcgttta tcaatttgcg ccgacccggt
     2461 aatacgagaa gatccctgat attcctcgcg ccattgctgg tagatcttgt ttgcctgcgg
     2521 tgtattgttt gtgccactaa aggttactac ttttccctgg tagccattgt tttccagaaa
     2581 atgtgcaagg tactgttgag ttcgcatcga ctccgtgaag atgatgactt tttttgcagc
     2641 ccccatttcg gcaagctgtg caaaaccttg ctccaggcca gaaagcaact cttttgattt
     2701 ggtatcagtg ccaatctgtt ctgctttaac aatgagtgat tccagctcat tgatttctgc
     2761 ctgaagcgca tctgaatcca cagatttgca ctctgtatct ggcgctactg attcggcatc
     2821 ttcaacgatg tcatcttcaa gatcatcatc cattatgatc tgttggatga tgtccatatc
     2881 atcttcaacg ataccttcca gctttaacct ttctaaacga gtcttaagga tctgcaagtt
     2941 attcaatacg gctttcgtac ttgaggaaag tagttttctg agtataagcc ccgtcagatg
     3001 ccggtgtcgt tttggtaagg catagctatc ctcacgttca agcaattttt gtacgcgttc
     3061 ataaagatcc tgctcctgtt cgctcggata gaaaggaata gtgatcgctt tacgttctgt
     3121 atacttgata tactccagca cattcttgcg tagagttcgt ttgataaaac gcgacaatcg
     3181 ccccttcaac tccgcaatat cgctatctgc ctgcatatat tgctttctga acgctttaac
     3241 ttcgccgaag gtatgttcat ctataagcgt cgacatgcca tacagttcca tcaacgagtt
     3301 ctgtaaaggc gttgcggtca gtagtaattt cttccgccca tcaagcgcac gctttaacgc
     3361 ttgccccatt ttgttgcttt ctcgatgagc attacgcagt ttatgggctt cgtcgataac
     3421 aaccagatcc cacatttcag ccaccaattt ctcttctaac cgagcagcat agtggtatga
     3481 catgatagtg atcgatttat tatttaaggg attatggatc ccttcttttt gaagctggct
     3541 atacattttc gcgtccaaca cctgagaagg tagattgaac ttttcctgta actcgctcgc
     3601 ccattggcga cgcagtgaag caggacaaat gatcaacagt ttacgttttc tttcagccca
     3661 caactggcaa agcactagtc cagcttcaat ggttttaccc aaaccaactt catcggcaag
     3721 cacaacacct ttgcttagcg gattttcgat agcaaaaaga gccgcctcaa tttgatgagg
     3781 gtttagatct acgcttgcat caaacagcga ttgagacaga cggtccaccc cattgtttga
     3841 atgaagaatg gaaagttcat gcgcaaaata tttcgcatga taaggtgttg taccaatcat
     3901 cttttctctc tcttcgctgt actttggacg ataacctgct gaattgaatc aagatattaa
     3961 aaggatacgt caatagtggt tgatcattga agtaaggcca tctccggttg cgggactttg
     4021 acacataaaa tcagcaattt tacgcatcgt ttcatgacta aacaacgacg cactaagaat
     4081 gccactcgcc aaatgcaatc tatttctgtg ccattagacc aaggcaatga agttttatat
     4141 ctacttaatt ttcaggatga tgaatgaaca tcattgatgg aaatggggct tccgaagttc
     4201 ttagattatc agcgttacat gcaaacaaag tagtgaaaac acaggtacaa aaaaagccgc
     4261 ccttgagcga ctcgatttgc atacgatgtg gtgcgaagac cggactcaaa catgcgattt
     4321 aacctcatga tttaaaattg ttaataataa acaacaccat cctataccaa cacagatacc
     4381 aacacgaaaa atcactggtt ttcggcatcg aaccaggaca atcaggtcct gattgagata
     4441 ccgagctctg ttactcgtca tcttcctcga caggctccat tgcatcaagt ggtactcgca
     4501 ccatcgcgat agaaccaatc cggataaaag catcttcccc atctatatcg gttaactgat
     4561 atcgcttact tgatgccgga taattgtgtc gattggcaac agtgtactct ttcgtacagc
     4621 cagaatgtgg cagactgagg tggaatagat aacgtccgta tgcccgctga ccacctccgg
     4681 gcgggagagt gtggtatctg acatcatcat ttttcctttc tgtttataaa tgaaaacgcc
     4741 agccgtgttc aggctgacgt cagggaagtg aaatcgggtg agtgatcttc actggttctg
     4801 gtgcaaaagt tactgttggc gcagggtacg gataccctcc ctggcctgtt cgatacagcg
     4861 caacagtgct gccgaacctg ttttatcctc atcgttgtcg aagataattc ccgattcgca
     4921 gtcgatattg tcctgcagcc acgtaatcag aatatccagc gctgtttccg tggttaatga
     4981 tttcatgttg tgaatttccg gattaccagt cgaaagtggg taaacccggc agatatccgg
     5041 cactggcatc cagatgaatg agactgacac cataacgccg gatgagtgtg acgaccagac
     5101 gacggaacgt ttttgacagc cccaggcgct taagccgcag aaccgggtat gaccacgcat
     5161 cggtacgtaa cagataaccg gtaccggtaa aatgaatcca ttctgattca ccgaagtcac
     5221 tggtctggtg tgacagcgag tacagccagg cgttgtccgt ttccgtgata tgtgcggtac
     5281 tgcagcgtat gccggtaagg gcaacaaatg gtggcgtgca gtctgctgag gtccggtcag
     5341 attcatcaac aacacgaagg gagtagccgt tatcgcagac catgttaatc agttcagcga
     5401 gattaacacc atcaatgtca acgacaattc gccccatatt cagtgcctgt acgttaacac
     5461 tgtcggcttc cagtgtcagg gccagtttca tcatttcgcc tccggatact tacccagggt
     5521 gatattgttc accgttctgt agttgtggcg ggtcatcagg cctgttgccc ggcgagcccg
     5581 gagaatatca atgctgttga ttaactgcga cgaggctcct gcgctgaatc ccggctggtc
     5641 agttcgtacc agtgcgtatt tttccacgag aaagttcact gcatcacaca gtgaaatgcc
     5701 tgcctcaata tgctgctcaa tcacgcgttc atccacgaac ggtgtgtcat tcagcgtaag
     5761 gctgtaatgc tggtccagta accgggtgag cagtgtctgc cagatggtga cgggagacgg
     5821 gcagcacgat acctcccgta catgcgtgtc gggtaatgtt ttcatactga agattttcct
     5881 gatatgcaga gataaaaaat gggacagtgg cgtggtgaaa acaccaggcc ggagcagaag
     5941 gttattctgg tggtttactt tttcgtttca ggtgtcggat aaacagccag ataaacgtaa
     6001 ccacaggagc cgagggtgtc tgcttcacag gtcagcccct tcgcatacag tgtcacggta
     6061 tgctgatggc ggggattcag ttcaccactg gtgagcatta atttcagttg tttcatcagc
     6121 agcggaaagg cctggtccag agggtacgca tccgcatctc tgaaccggcc tctgataccg
     6181 gcacggtcgg caaggtaatg caaccggtta ccctcctgca ccagacgggc gccgaaacag
     6241 ggcgtcactg tgcagggtag tccccaccag ggatggtcgt cattgtcatc gggatgcgtt
     6301 gtcccggaaa gtgtgtctga cacgataaaa tcctcacaga aaatcggtga atgatagttt
     6361 aacgatgacg ggtcagccgg tgcacagacc ggcttcggtg aaggtgtatt cgttgatgag
     6421 cagggcctca tccacagcgg cgtctgagct cagccagtcg tactcgtttt ccgggtgacg
     6481 gaacatcagt tcagggtgac gctcatcagc cggacatacg gaccaaaact gtccttacgg
     6541 cgttctgcaa acacggccag cacaccggga atatcctgca cttcacgacc ggtatacgct
     6601 tcagcactgc cgtgccagcg gtatttgccg gtacagaacg gaaaaagacg ggatgccgga
     6661 tgctgacggt gaatacgcat ggcttcgcca cgggtgatga ttttcatagt gggatacctc
     6721 tgaagacaga agataaaagt gaaaacaggt gtgatgtggt tgtgacgggt taaagcagac
     6781 catgttcggc aaaggagaaa acctggctgc caccgactat cagatggtcc ggcacccgga
     6841 tatccaccag gcccagtgcc tgtaccagac gttccgtgat aaggcggtct gccttgctgg
     6901 gtgtgacttc accggacggg tgattgtgtg ccagcaccac agcagcggca ttgtggtaca
     6961 gggcgcgttt aatcacttca cggggatgga cttccgtgcg gttgatggtg ccggtgaaga
     7021 gggtttcacc ggcaatcagc tgattctggt tgttcagata cagcacccgg aactcttcac
     7081 gctccagtcc cgccatgttc agaatcagcc attctcgtgc cgcacgggtg gaggtgaagg
     7141 ccacgccggg ttcatgaaga tggcggtcca gggttttcag ggcccgttga atgaggctgc
     7201 gctcgccggg cgtcatctct ccgggcagaa aggaaagctg ttgcattgta cttctctcca
     7261 ttcagtcgat gatgcgcata atggcgctgc attccggatg ctgcagggcg taatcccgca
     7321 gccggtaata atggaccgtc atggcataac actccgtacg acaggcatga tgactgtacg
     7381 tcatcagaca ggcagcaatg ccggcagctt ccggactcat ttcagcgcgg ttaccgttca
     7441 tggcattgaa cagtacccat gtttcgtcat catcgtcatc cggttcgggt gccataaatg
     7501 ccccgccgtt gttcagggtg tacagattcc agataccacc gcagtagtct tcacacagac
     7561 ggtccatcca gccgaagaca cggggctcca gggtcaccca ctgtggaatg aggccaaagt
     7621 gctgcggcca gaagctgatg cgctgttcat cagggacgag ggtggcaacc aactgaggct
     7681 ggtcattccc tgatgcagcg gttacggaaa cagaagatgt ggtggaatta tgtgaaatgg
     7741 ttttcatgaa atgactcctt aaaggacaaa taaatgcgtg gttttcaggg taatggctga
     7801 atcagcgcag gctctccagc agggtttccg ccatcaccca caatgcgcgg ttgagcttaa
     7861 tgtcagtgtc aatgctgtga atggcacggg tatggatacg ttttccttta gcgctgcgac
     7921 ctgaaattcc gcctttcagc atattctcct gaatggtctg ataagcactc cacaggtcct
     7981 taccgtaatc ctcccggcgt cgtggcgtca gaatgtcggc ggtggtgacg ggctgatgtt
     8041 cgtcaccata acggtaagtc agtgccgcct gtgccagcgc ctggcgtgcc ggtggcggca
     8101 gaatcagcga ctgcatggca tcacgctttt cctcaatccg gtcaaacacg cccaccacct
     8161 cgtaagcccc ttcaatgact ctgtccacca catttccccg gtgcggaaca cgcacttccc
     8221 ccagagactg accacagacg catccgttct ggcagacgaa cctgaagtaa cccggcagca
     8281 tctggtagct ggaggtaccg tcatgagagt tgagcagaat aatttcaggg acatgttctc
     8341 cgtttatctc tccggcccgc cgcagacgca gcat
//
LOCUS       DBANUU010000167         8366 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000167 DBANUU010000000
VERSION     DBANUU010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.169"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(87..293)
                     /locus_tag="ACIE68_004084"
     CDS             complement(87..293)
                     /locus_tag="ACIE68_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFX5910313.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            606..695
                     /gene="kdpF"
                     /locus_tag="ACIE68_004085"
     CDS             606..695
                     /gene="kdpF"
                     /locus_tag="ACIE68_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFX5910314.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            695..2368
                     /gene="kdpA"
                     /locus_tag="ACIE68_004086"
     CDS             695..2368
                     /gene="kdpA"
                     /locus_tag="ACIE68_004086"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFX5910315.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2391..4439
                     /gene="kdpB"
                     /locus_tag="ACIE68_004087"
     CDS             2391..4439
                     /gene="kdpB"
                     /locus_tag="ACIE68_004087"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFX5910316.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMCLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4448..5020
                     /gene="kdpC"
                     /locus_tag="ACIE68_004088"
     CDS             4448..5020
                     /gene="kdpC"
                     /locus_tag="ACIE68_004088"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFX5910317.1"
                     /translation="MSGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVINIVELNLALDKLDE"
     gene            5013..7685
                     /gene="kdpD"
                     /locus_tag="ACIE68_004089"
     CDS             5013..7685
                     /gene="kdpD"
                     /locus_tag="ACIE68_004089"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFX5910318.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRTQGLDIVVGVVETHGRKDTAAMLEELAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLGSV
                     WHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYAREHNLG
                     KIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFKDKWR
                     VQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVVATVI
                     NVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYREQRT
                     RHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQGMTPW
                     DDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMIPEQQ
                     RLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVLFGQA
                     EILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLTLEEV
                     VGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAEIGID
                     AHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIVDVHG
                     GTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7682..8359
                     /gene="kdpE"
                     /locus_tag="ACIE68_004090"
     CDS             7682..8359
                     /gene="kdpE"
                     /locus_tag="ACIE68_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFX5910319.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
BASE COUNT         1740 a         2182 c         2443 g         2001 t
ORIGIN      
        1 tctaaatatt cctctgaaca tcttcctgat acaaaaaacc gccagcgttt ccgctggcgg
       61 ttttcaatgt attcaaaact cagcaatcaa taaaaatcac cagttgcctt ttcggcctga
      121 tccatccaca ccggtttatc gctggttttc gaccagacgc gatgcaggta gctgtaaaag
      181 cgggcgcggt ctttccagaa aagcatgaaa ggcagcgcca gaacgcccgc tgcaaccgca
      241 taagtacggc gtaaaaagat aagccatgca ggatattctc tgtagagttc catacttttc
      301 tccccctttt gtatctaccc ggtgaatggc accggaaaaa tgaatttgtt tatctgatga
      361 aaatagtacc gccttttgtg taattttact actcatccga ccacttattt ttgcttattg
      421 atggtttatt tacattcatc ctgtaattaa gttacacaaa agttaaatta atactaaaca
      481 ttagttaaat catggctttt gccattttta tacttttttt acaccccgcc cgcagatttt
      541 tgcgaaatct ttgcagccag aattctaccc ttccggtatc acttttaggc cactggaggt
      601 gcactgtgag tgcaggcgtg ataaccggcg tattgctggt gtttttatta ctgggttatc
      661 tggtttatgc cctgatcaat gcggaggcgt tctgatggct gcgcaagggt tcttactgat
      721 cgccacgttt ttactggtgt taatggtgct ggcgcgtcct ttaggcagcg ggctggcgcg
      781 gctgattaat gacattcctc ttcccggtac aacgggcgtt gagcgcgtac tttttcgcgc
      841 acttggcgtc tctgaccgtg agatgaactg gaagcaatat ctctgtgcca ttctcggcct
      901 gaacatgctg gggctggcgg tgctgttttt tatgttgctc ggtcagcact atctgccgct
      961 taatccacag cagttgccgg ggctgtcgtg ggatctggcg ctgaataccg ccgtcagctt
     1021 tgtcacgaat accaactggc aatcttacag cggtgaaacc acgttgagct atttcagcca
     1081 gatggctggc ttaacggtgc aaaactttct ttctgccgcc agcgggattg cggtgatttt
     1141 tgccctcatc cgtgcgttta cccgccagag catgagcaca ctcgggaatg cctgggtcga
     1201 tctgctacgc atcacgttat gggtgctcgt ccctgtggcg ttgttgatcg cactgttttt
     1261 tattcaacaa ggtgcgctgc aaaactttca gccttatcag gctgtgaata ccgttgaagg
     1321 agcgcaacag ctgttaccca tggggcctgt agcttctcag gaagcgatca agatgctcgg
     1381 tactaacggc ggtggcttct ttaatgccaa ctcgtcgcat ccgtttgaaa acccaaccgc
     1441 actgaccaac ttcgtgcaga tgctggcgat cttcttgatc ccaacggcgc tgtgctttgc
     1501 ctttggtgaa gtgacgggcg atcgccgcca ggggcgcatg ttgctgtggg cgatgtcagt
     1561 gatttttgtc atctgcgtag gcgtggtgat gtgggcagaa gttcagggta atcctcatct
     1621 gctggcgctc ggcgcagaca gcagcatcaa tatggaaggt aaagagagcc gtttcggcgt
     1681 gctggtcagt agcctgtttg cggtcgtgac tacggcggct tcctgtggcg cggtgattgc
     1741 gatgcatgat tcgtttaccg ctctcggtgg catggtgccg atgtggctga tgcaaattgg
     1801 tgaagtggtg ttcggcggtg tcggttctgg tctttacggc atgatgctgt ttgtcctgct
     1861 ggcggtgttt attgccgggc tgatgattgg tcgtacaccg gaatatctgg gtaaaaaaat
     1921 cgacgtacgc gagatgaaac tgactgcact ggcgattctg gttaccccga cgctggtgct
     1981 gatgggcgcg gcgctggcaa tgatgaccga cgccggacgt agcgccatgc tcaaccctgg
     2041 cccgcatggt tttagcgaag tgctgtacgc cgtgtcatcc gccgctaaca acaacggcag
     2101 cgcctttgcc ggattaagcg ccaactctcc gttctggaac tgtttactgg cgttctgcat
     2161 gtttgtcggt cgcttcgggg tgattatccc ggtgatggca attgccggtt cgctggtgag
     2221 taaaaagagc caacccgcca gctccggcac gctgccaacg cacggcccgc tgtttgttgg
     2281 cctgttaatc ggcaccgtgt tgctggttgg cgcactgacc tttatccctg ccctggcgct
     2341 tggtccagtg gcggaatatc tctcctgatg atattgagtg agcactgaat atgagtcgta
     2401 aacaactggc gctattcgaa ccaacacttg tcgttcaggc gctgaaagaa gcggtgaaaa
     2461 aattaaaccc gcaggcgcaa tggcgcaatc cggtgatgtt tatcgtctgg atcggcagtc
     2521 tgctgaccac ctgtattagc atcgcgatgg caagcggtgc gatgcctggc aatgcgctgt
     2581 ttagcgcggc aattagcggc tggctgtggg tcaccgtact gttcgctaat ttcgccgagg
     2641 cgctggcaga aggccgcagt aaagcgcagg ccaacagtct taaaggggtg aaaaaaactg
     2701 cctttgcccg caagctgcgt gagccgaaat atggcgctgc ggcggacaaa gttcctgccg
     2761 atcaactgcg taaaggtgac atcgtgctgg tagaagctgg cgatattatc ccctgcgatg
     2821 gtgaagtcat tgaaggcggc gcatcggtcg atgaaagcgc catcaccggg gaatcggcac
     2881 cggtgatccg tgaatccggc ggcgattttg cctccgtcac cggcggcacg cgtattcttt
     2941 ctgactggct ggtgattgag tgtagcgtta accccggtga gacatttctg gatcggatga
     3001 tcgcgatggt ggaaggcgca cagcgacgca aaacaccgaa cgagattgcc ctgactatcc
     3061 tgctgattgc tctgaccatc gtctttttac tggcaaccgc cacgctgtgg ccgttttccg
     3121 cgtggggcgg taatgcagtc agcgtaacgg tactggtggc gctgctggtc tgtctgatcc
     3181 caaccactat tggcggcctg ttgtcagcga tcggcgtcgc cgggatgagc cggatgctag
     3241 gcgcgaatgt gattgccacc agcggacgtg cagttgaagc ggcaggtgac gttgacgttc
     3301 tgctactgga taaaaccggc accatcacac tcggtaaccg tcaggcgtcg gagtttatcc
     3361 ccgcgcaggg cgtggatgaa aaaacgctgg ctgacgccgc acaactggct tcgctggctg
     3421 atgaaacgcc ggaaggccgc agtattgtga tcctcgccaa gcagcgtttt aacctgcgcg
     3481 agcgcgatgt gcagtcgctc catgccacct ttgtaccgtt tactgcgcaa agccggatga
     3541 gcgggatcaa catcgacaac cgcatgatcc gtaaaggttc tgtcgatgcc attcgtcgcc
     3601 atgttgaggc taacggtggt cacttcccta ccgatgttga tcaaaaagtc gatcaggttg
     3661 cgcgtcaggg agccacgccg ctggtggtgg tggaaggttc tcgtgtgctg ggcgttattg
     3721 cgctgaaaga tatcgtcaaa ggcggtatta aagagcgctt cgcccagctg cgcaaaatgg
     3781 gcattaaaac ggtgatgatt accggcgata accgtctgac tgccgccgcg attgctgcgg
     3841 aagcgggtgt cgatgatttt ctcgccgaag cgacaccgga ggccaagctg gcattgatcc
     3901 gtcagtatca ggcggaaggt cgtttggtag cgatgaccgg cgacggcacc aacgatgctc
     3961 cggcgctggc gcaggcagat gtcgcggtgg cgatgaactc cggcacccag gcggcgaaag
     4021 aggcgggcaa tatggtcgat ctcgactcta acccgaccaa gttgatcgag gtggtgcaca
     4081 ttggcaaaca gatgctgatg acccgtggct cgctgaccac cttcagcatt gccaacgatg
     4141 tggcgaaata cttcgccatt attccggcgg cattcgcggc aacgtatccg cagttaaatg
     4201 cgctgaacat catgtgcctg cattcgcccg actccgcaat cctcagtgcg gtgattttca
     4261 acgccttgat tatcgtcttt ttgattcccc tggcgttaaa aggcgtgagt tataaaccgc
     4321 ttaccgcttc tgccatgttg cgccgtaact tatggattta cggtctgggt gggctgctgg
     4381 tgccgtttat cggtatcaaa gtcattgatt tactgctgac cgtttgcggt ctggtgtgag
     4441 gtttaccatg agtggattac gtccggcatt atcaacattt atctttctgt tattgattac
     4501 tggcggcgtt tacccgctgc tgaccaccgt actggggcaa tggtggtttc cctggcaggc
     4561 caatggttcg ttgattcgtg aaggtgatac ggtgcgcggt tcggcattaa tcgggcagaa
     4621 ttttaccggc aacggctatt ttcatggtcg cccgtcggca acggcagaaa tgccctataa
     4681 tccacaggct tctggcggga gcaatctggc ggtcagtaac cctgagctgg ataaactaat
     4741 agccgcacgc gttgctgcat tacgggccgc taacccggat gccagcgcga gcgttccggt
     4801 tgaactggtg acggcatcgg caagcgggct ggacaataat atcaccccgc aagcggcggc
     4861 ctggcaaatc ccacgcgtgg cgaaagcgcg taatctcagc gttgaacagc tcacgcaact
     4921 gatcgcaaaa tacagccaac aaccgctggt gaaatatatc ggccagccgg ttatcaacat
     4981 tgttgaactc aatctggcgc tggataaact tgatgaataa cgaaccctta cgtcccgacc
     5041 ccgatcgtct gctggaacaa actgccgcgc cgcatcgggg gaagctgaaa gttttcttcg
     5101 gtgcctgtgc aggcgtcggg aagacctggg cgatgctggc agaagcacag cgactgcgga
     5161 cgcaagggct ggatattgtg gttggcgtgg tagaaaccca cgggcggaaa gataccgccg
     5221 ccatgctgga agagctggct gttctgccgc caaaacgcct ggcgtaccgt gggcggcata
     5281 tcagcgagtt tgatctcgat gccgccctcg cccgccgtcc ggcgctgatc ttaatggacg
     5341 aactggcgca cagtaatgcg cctggttctc gtcatcccaa acgctggcag gatattgaag
     5401 agctgctgga agcaggcatt gatgttttta ctaccgtcaa cgttcaacat ctggaaagcc
     5461 tgaatgatgt ggtcagcggc gtcaccggaa ttcaggtacg ggaaaccgtg cccgatcctt
     5521 ttttcgatgc cgccgacgac gtggttctgg tggacttgcc cccggacgat ctgcgccagc
     5581 ggctgaaaga aggcaaagtc tatattgccg ggcaggcgga gcgcgccatt gaacattttt
     5641 tccgcaaagg caatctgatc gccctgcgcg aactggcact gcgccgtact gccgatcgcg
     5701 tagatgagca aatgcgcgcc tggcgggggc atcctggcga agagaaagtg tggcacacgc
     5761 gcgacgcgat ccttttatgc atcgggcata acaccggcag tgagaaactg gtccgcgcgg
     5821 cggcgcggct gggtagcgtc tggcacgcgg tgtatgttga aacccctgcc ctgcatcgct
     5881 taccggagaa aaaacgtcgg gcaattctca gcgccttacg tctggcgcag gagctgggcg
     5941 cggagacggc aacactttct gatccagcgg aagagaaagc ggtagtgcgt tatgcccgtg
     6001 aacataatct cggcaagatt attctcggtc gcccagcctc gcgccgctgg tggcgtcggg
     6061 aaacgtttgc tgaccgactg gcgcgcatcg cccccgatct cgatcaggtg ctggtcgcgc
     6121 ttgatgaacc acccgcccgc acgattaaca acgcgccgga tagccgctct tttaaagaca
     6181 agtggcgggt acagattcag ggatgcgtgg ttgccgccgc gttatgcgcc gttatcactt
     6241 taattgccat gcagtggctg atggcgtttg atgccgccaa cctggtgatg ctgtatctgc
     6301 ttggcgtggt ggtagtggcg ctattttatg gacgctggcc ttcggtggtt gctaccgtca
     6361 ttaatgtggt gagtttcgat ctctttttta tcgccccacg cggcacgctc gccgtctctg
     6421 atgtgcaata tctgctgacc ttcgcggtga tgttaaccgt cgggctggtg atcgggaacc
     6481 ttactgctgg cgtgcgttat caggcgcggg tagcccgtta ccgcgagcaa cgcacacggc
     6541 acttatatga aatgtcgaaa gctctggcgg tgggccgcag tccgcaggac atcgccgcca
     6601 ccagcgaaca atttattgcc tccacgtttc atgcccgcag tcaggtgttg ttgcccgatg
     6661 acaacggtaa attgcagccg ttaacacatc cgcaaggaat gacgccatgg gacgatgcca
     6721 tcgcgcagtg gagttttgat aaaggcctgc ctgcgggtgc gggcaccgac acgttacccg
     6781 gcgtgccgta ccagattttg ccgctaaaaa gcggcgagaa aacctacgga ctggtggtgg
     6841 tggaaccggg gaatctgcgc cagttgatga tcccggagca gcagcgcctg ctggagacat
     6901 ttacgctgtt agtcgccaat gcccttgagc ggctgacact gaccgccagc gaagaacagg
     6961 cgcggatggc aagcgaacgt gaacagatcc gcaacgccct gctggcggcg ctttcgcatg
     7021 atttacgcac gccgcttacg gtgctgtttg gtcaggcaga aatcttaacg ctcgatctgg
     7081 caagcgaagg atcgccccac gcccgccagg ccagcgagat tcgtcagcat gtgctgaaca
     7141 ctacccgact ggtgaataat ctactggata tggcgcgaat tcagtccggc ggctttaatt
     7201 tgaagaaaga gtggttaacg ctggaagaag tggtcggtag cgcgctgcaa atgctggaac
     7261 cgggtttatc gtcgcccatc aatctttctc tgccagaacc gctgacctta atccacgttg
     7321 acgggccact ctttgaacgg gtgctgatta atctgctgga gaacgcggtg aaatatgcgg
     7381 gtgcgcaggc cgaaattggt atcgatgccc acgttgaggg cgaaaatcta caactggatg
     7441 tctgggataa cggccccggt cttccgccag gccaggagca gacgatattt gataagtttg
     7501 ctcgcgggaa taaagagtcg gcagtaccgg gggtagggct tggactggca atttgtcggg
     7561 cgatagtgga tgtacacggg ggcactatta ccgcgttcaa ccgaccggaa ggtggtgcct
     7621 gttttcgtgt tacacttccc cagcaaaccg ctcctgaact tgaagacttt catgaggata
     7681 tgtgacaaac gttctgattg ttgaagatga acaggctatt cgtcgctttc tgcgcacggc
     7741 gctggagggc gacgggatgc gcgtctatga ggccgaaacg ctgcaacgcg gcttgctgga
     7801 agcggctacc cgtaagccag atttgattat tctcgatctc ggcctgcccg atggtgatgg
     7861 gattgagttt atccgcgacc tgcgccagtg gagcgcggtg ccggtgattg tgctttccgc
     7921 acgcagcgaa gagagcgaca aaatcgccgc gctggatgcc ggagcggatg attatctgag
     7981 taagccgttt ggcattggcg aattgcaggc ccgtctgcgc gtcgctttac gccgccactc
     8041 cgccaccgcc gcgcccgatc cgctggtgaa attttccgat gttaccgtcg atttagccgc
     8101 ccgcgtgatt caccggggtg aggaagaggt gcatctcacg ccgattgagt tccgcctgct
     8161 ggcggtgctg ctgaataatg ccgggaaagt gctcacccag cgccagttac tcaatcaggt
     8221 gtgggggcca aacgcggtcg aacacagtca ctatttgcgt atttatatgg gacatctgcg
     8281 acaaaaactg gaacaggatc ccgctcgccc ccgccatttc attactgaaa ccggtattgg
     8341 gtatcggttt atgccatgaa tgttta
//
LOCUS       DBANUU010000168         8337 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000168 DBANUU010000000
VERSION     DBANUU010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.170"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(116..192)
                     /locus_tag="ACIE68_004091"
     tRNA            complement(116..192)
                     /locus_tag="ACIE68_004091"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156..158),aa:Asp,seq:gtc)
     gene            complement(325..1056)
                     /gene="dnaQ"
                     /locus_tag="ACIE68_004092"
     CDS             complement(325..1056)
                     /gene="dnaQ"
                     /locus_tag="ACIE68_004092"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFX5910320.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DDELAAHEARLDLVQKKGGSCLWRA"
     gene            1121..1588
                     /gene="rnhA"
                     /locus_tag="ACIE68_004093"
     CDS             1121..1588
                     /gene="rnhA"
                     /locus_tag="ACIE68_004093"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFX5910321.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1585..2307)
                     /locus_tag="ACIE68_004094"
     CDS             complement(1585..2307)
                     /locus_tag="ACIE68_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFX5910322.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2341..3096
                     /gene="gloB"
                     /locus_tag="ACIE68_004095"
     CDS             2341..3096
                     /gene="gloB"
                     /locus_tag="ACIE68_004095"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFX5910323.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3168..4526
                     /gene="mltD"
                     /locus_tag="ACIE68_004096"
     CDS             3168..4526
                     /gene="mltD"
                     /locus_tag="ACIE68_004096"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFX5910324.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4574..5344)
                     /locus_tag="ACIE68_004097"
     CDS             complement(4574..5344)
                     /locus_tag="ACIE68_004097"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFX5910325.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDISHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGFFTSDIIMVDAHKAA"
     gene            complement(5422..6222)
                     /locus_tag="ACIE68_004098"
     CDS             complement(5422..6222)
                     /locus_tag="ACIE68_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFX5910326.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6463..7377
                     /gene="yafC"
                     /locus_tag="ACIE68_004099"
     CDS             6463..7377
                     /gene="yafC"
                     /locus_tag="ACIE68_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFX5910327.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMASAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7374..8177)
                     /gene="dkgB"
                     /locus_tag="ACIE68_004100"
     CDS             complement(7374..8177)
                     /gene="dkgB"
                     /locus_tag="ACIE68_004100"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFX5910328.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVITALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         2228 a         2003 c         2028 g         2078 t
ORIGIN      
        1 acgcacttac gccagagtaa aatcattgaa cagagaaatg gtggaattca aatacaaaaa
       61 aaccgcaaaa ttaaaaatct tgcggctctc tgaactcatt ttcatgagtg aatagtggcg
      121 gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag gcaggtattc taaccgactg
      181 aactaccgct ccgcgttgtg ttccgttggg aacgaggcga atattacgga ttgcctcgac
      241 cttcgtcaac ggtttttctc atcttttgaa tcgtttgctg caaaaatcgc ccaagtcgct
      301 atttttagcg ccttttacag gtatttatgc ccgccagagg cagcttccgc ccttcttctg
      361 caccagatca agacgggctt catgtgctgc aagctcgtca tctgtcgcaa aaacaacgcg
      421 taacttactt gcctgacgta caatgcgctg aattgttgct tcaccttgtt gctgttgtgt
      481 ctctccttcc atcgcaaaag ccatcgacgt ttgaccaccg gtcatcgcca gataaacttc
      541 cgcaaggatc tgggcatcga gtaatgcccc gtgtagcgtt cgtttactgt tatctatttc
      601 gtagcgggca cataacgcat cgaggctgtt gcgcttacca ggaaacattt tcctcgccac
      661 cgcaaggcta tcggtgacct tacagaaggt attggtcttc ggaatatcgc gcttaagcaa
      721 cgaaaactcg tagtccataa agccgatatc gaacgctgcg ttatggatca ccaactccgc
      781 gccgcggata tagtccatga actcatcggc tacttcggca aacgtgggct tatcgagcaa
      841 aaattcatcg gcaataccat gtacgccaaa ggcttcagga tccaccagcc gatcgggttt
      901 gagataaaca tggaagttat tgcccgtcag gcgacggttc accacttcaa cggcaccaat
      961 ctcaatgatc ttgtggcctt catagtgcgc accaatctgg ttcataccgg tggtttcggt
     1021 atcgagaacg atctggcgtg taattgcagt gctcatagcg gtcatttatg tcagacttgt
     1081 cgttttacag ttcgattcaa ttacaggaag tctaccagag atgcttaaac aggtagaaat
     1141 tttcaccgat ggttcgtgtc tgggcaatcc cggacctggg ggttatggcg ctattttacg
     1201 ctatcgcgga cgcgagaaaa cctttagcgc tggctacacc cgcaccacca acaaccgtat
     1261 ggagttgatg gccgctattg tcgcgctgga ggcgttaaaa gaacattgcg aagtcatttt
     1321 gagtaccgac agccagtatg tccgccaggg tatcacccag tggatccata actggaaaaa
     1381 acgtggctgg aaaaccgcag acaaaaaacc agtaaaaaat gtcgatctct ggcaacgtct
     1441 tgatgctgca ttggggcagc atcaaatcaa atgggaatgg gttaaaggcc atgccggaca
     1501 cccggaaaac gaacgctgtg atgaactggc tcgtgccgcg gcgatgaatc ccacactgga
     1561 agatacaggc taccaagttg aagtttaagc ctgtggttta cgacattgcc gggtggctcc
     1621 aaccgcctgg cgaattcgtg gcttgttttt actctgtttc atcggattta gcgttaaagg
     1681 aatagtccgt ttccgggcaa caataagttg taagcaacca agcgcaggaa tatgcgcatt
     1741 caatagtttt cctccgtgtt tgttccacgg gagaacgtgg aaacggctgg cgtgtagcac
     1801 ttcaaaattc aacaaagaga gccagtccag ctgccgcatc agagtaaaca tccggctgtt
     1861 atagggcgag gttttgcgca ataccggcac aagtttgcgt aatcccatta aactgatggg
     1921 attgaagcca ctaatgacca gccagccatc atcaatcaat acccgatcgg cttcacgcaa
     1981 taaacgatgc ggatcggtgc accacggcaa tgtatgtgcc agtagacaaa catcaacgga
     2041 tttatcggca aaaggaagat gaagtgggtc cgcctgtacc tggacgggca ttccttgcgc
     2101 agaaacattc acttgatgag aaaccgcgca cgcttcgcaa ttgatttctg cgcttaaatt
     2161 gccaatctta agcagatgaa aaccatacat tttagtgaac cacgggttga gctggcgctc
     2221 cagcgccttg cgataaagct ctccccaggg caaatcgccc cagcaatcag gagccacgac
     2281 agtttgaggg actcttgccg gtttcatcac aaccttccgt ttcacactga gaggtaatct
     2341 atgaatctta acagtattcc cgcctttgat gacaattaca tctgggtttt gaatgatgaa
     2401 gcaggtcgct gcctgattgt cgatcccgga gacgcagagc cagtattaaa cgccattgcc
     2461 gccaataact ggcaaccaga ggccatattt ctcacccacc atcaccacga tcacgttggc
     2521 ggcgtaaaag aactggtgga aaagtttccg caaattgtgg tgtatggtcc acaagagaca
     2581 caagataagg gaacaacaca ggtagtcaaa gatggcgaaa ctgccttcgt tttagggcat
     2641 gaatttagtg taattgccac accgggtcac actttaggac atatctgtta cttcagcaaa
     2701 ccttatctat tttgcggcga cacgctgttt tctggtgggt gtggtcggtt gtttgaaggg
     2761 acaccatcac aaatgtatca atcacttaaa aagttaagtg cgttacctga cgatacattg
     2821 gtatgttgtg ctcatgaata tacgttatca aatatgaagt ttgctttgag tattcttccg
     2881 cacgatttgt ccataaatga ttattatcgt aaagttaagg agttacgggc aaaaaatcaa
     2941 ataacactac ccgtaattct gaaaaatgag cggcaaatta atgttttttt aagaacagaa
     3001 gatattgatt taattaatgt aattaatgaa gaaacattat tgcaacaacc tgaagaacgt
     3061 tttgcatggt taaggtcaaa gaaagatagg ttctgataaa actttcttgt catcggctcc
     3121 gttcgccgtt atgatcggtc gtcttttaag caactattga cacacacatg aaggcaaaag
     3181 cgatattact cgcctctgtc ctgctcgtgg gctgccaaag taccggcaac gttcaacagc
     3241 acgcacagag cctttctgca gctggtcaag gggaagcagc aaagtttaca agtcaggcac
     3301 gatggatgga cgatgggacg tctatcgcgc cagatggtga cttgtgggct ttcattggcg
     3361 acgagctaaa gatgggaatt ccggaaaatg accggattcg cgaacagaaa cagaaatatt
     3421 tacgcaataa gagctatctc cacgatgtaa ctttacgggc agagccgtat atgtactgga
     3481 tagccgggca agttaaaaaa cgtaacatgc ctatggaact ggtactacta cccatagtgg
     3541 agagcgcttt tgatcctcac gcaacgtctg gcgccaatgc cgcaggcatc tggcagatca
     3601 ttccgagcac ggggcgcaat tatggtttga aacagacccg caattatgac gcgcgtcgcg
     3661 atgttgttgc ttcaacaact gccgcgctga acatgatgca gcgtctgaac aagatgtttg
     3721 acggcgactg gcttctgacc gtagcggctt ataacagcgg cgaaggtcgg gtcatgaagg
     3781 caattaaaac gaacaaagcg cgtgggaaat ccacggactt ctggtcgtta ccgttgccgc
     3841 aggaaacgaa gcagtacgtg cctaaaatgc tggcattgag tgatattctc aaaaacagca
     3901 agcgttatgg cgtacgtctg ccaacgaccg atgaaagccg tgctctggcg cgtgtgcacc
     3961 tgagcagccc ggttgaaatg gcgaaggttg cagatatggc ggggatttcc gtcagcaagc
     4021 tgaagacatt caacgctggc gtaaaaggct ccacgctggg cgcaagtggc ccgcagtacg
     4081 tgatggtgcc aaagaagcat gcagatcaac tgcgtgaatc tctggcttca ggcgaaattg
     4141 ctgctgtaca gtcgacgctg gttgccgaca atacgccgct taacagccgt gtttacaccg
     4201 tacgctctgg cgacacgctt tcaagtatcg cttcacgtct cggcgtaagc accaaagatt
     4261 tgcagcagtg gaacaaactg cgcggatcta agctgaagcc aggccaaagt ttgacgattg
     4321 gtgcaggcag tagcgcacag cgactggcaa acaacagcga tagcattacg tatcgtgtgc
     4381 gcaaaggcga ttcgctttca agcattgcta aacgccacgg cgtgaacatc aaagatgtga
     4441 tgcgctggaa cagcgacact gcgaatctgc aaccaggcga taagctgacg ttgtttgtga
     4501 aaaacaacaa catgccagat tcctgacaaa ccagataata aaaaggcacc gattcccccg
     4561 gtgccttttt tatttatgcc gctttatgtg catctaccat gatgatatca ctggtgaaaa
     4621 agccatcatt ctgcaaggca aaatacgttc tcacctctgt cgatgcgctc tgttggtaaa
     4681 tgcgaatagc gtctactaac gcttctggcg tacgcattct cgcgacccat gaagaaaatt
     4741 ccagcggtaa cttatctgta attaaattat caactatcag attggcttca ttgattaacg
     4801 tcaaccactc accgctggcg tagtttcgta cgtgagagat atcgcgtaat gcttctaccg
     4861 tctgtaacca gatgtcgcgc actgggtgac ccggagacat tacgtccatc acaatcagcc
     4921 taccgccagg tttcaatatc ctattcactt ctcgcagtgc tgcaccaaca tcatgccagt
     4981 gatgggcaga ataacggctg ataacaatat caaatgcgtt atcggcaaat ggcagacttt
     5041 cggcatatcc ctggcgggtg gtgatatttt tcagttgccg ggcttcggca gcttgtgcca
     5101 cgacatccag catatgggca gataagtcat acgccaccac cgtgctcacg ttttgcgcag
     5161 cgacaaagct ggcatgccct gctccgcagc ccatatcaag tacacttgca ccaggataat
     5221 cagccagacg caccgccagg cgctgtaaat ctcgtccgga tgcatgtacg gtactggtca
     5281 gatattcgca ggcttgagag ctaaactgct tttctacgtg gtcatggtgg gattgtgttg
     5341 tcattgcgct gtccttttgt tgtttgagta taaaaggaca gcgccaaacg ttggtctgcc
     5401 ctgtggcaga cctgacatac cttatttatc aggcttgccg ggactgaatt caacgagtag
     5461 cggattgtga tcggaagcgc gcgtaaccaa tacagaagct tcgctgacgt tcagaccacg
     5521 gtagaaaaca aaatcgagcg ggcggccaaa cgcccgacgg cgctgatcat cggtaaaacg
     5581 cacctggcgc agcgacattt cccgcgcaaa gcgatataac gcgttcatcc ttctacggct
     5641 ccaggcattg aaatctcccg ccataatgac cgggccgctg tggtgagcta tctgatcacc
     5701 aataggaagt aactgcttac tatagacatc cacgcccaga ctgaagttga cggcgtgtat
     5761 attaaccacc atcaacaggc gggtgtcagg taatggatag accgtcacca gtgccgactt
     5821 cgccagacgc aaaatgggtt ctcgttcacg taacgggcag caatacactg gatgcgccgc
     5881 cgaaagggtc attacaccgg aaggatgttg tggcagcacg aaagcgggca cctgatcggc
     5941 ggcaagatag ttagcggtcg caaactgtac taactctggc gttgtctgcg cttcctgcaa
     6001 taacaccaga tgtgcatctt tgccatagtt ctttaatacc gacaaccatt cagcgcgttg
     6061 ctgtttgtat atgttccaca ccaggatccg aatacgctct tcggtactta acggttctcc
     6121 aggtggtaat gcctggccga tgctcgcaaa agaccccggc ggtaagatcc tttccgcagg
     6181 ttgtccggca acatagcgca tggcataggt gttttttcgc acttttgact ttgaaacctc
     6241 tattacgtca acccttccgc actcgggagg gttctactgt tatagggatt ttagctcact
     6301 ctttcaacga gcaattgtat attgttatgt aagcaagtgc ttacaccggt cagaattttg
     6361 ctcattatgg agaacattaa atcgcatgac atatcacgca tccagatggt ttaatgacaa
     6421 ataaattcaa aatatctctt ctattttgca acaggagcaa aaatgaaagc cacgtcggaa
     6481 gaactcgcca tttttgtttc ggtcgtcgaa agcggcagct ttagccgggc agcggaacaa
     6541 ttagggcaag caaactcagc ggtaagccgg gcggtgaaaa agctggagat gaaacttggc
     6601 gttagcctgc ttaatcggac cacgcgacaa cttagcctga cggaagaagg cgagcgttat
     6661 tttcgtcgcg tacagtcaat tttgcaggag atggcatcgg cagaatcaga aattatggag
     6721 acgcgtaata caccgcgtgg actgttacgg atcgatgccg caactccagt ggtgctgcac
     6781 tttctgatgc cgttaattaa gcctttccgt gaacgctatc cggaagtcac tttgtcgcta
     6841 gtctcctccg aaacgattat taatttgatc gaaagaaaag tggatgtcgc gatacgcgct
     6901 ggtacgttaa cggattccag cttacgtgcc aggccgttat ttaacagtta tcgaaaaatt
     6961 atcgcctccc ccgattatat ttcccgctac gggaagccag aaacgatcga cgatttaaag
     7021 caacatattt gcctgggatt cactgaaccc gcttccctca atacctggcc gatagcccgt
     7081 agcgatggac aattacatga ggtgaagtac ggtttgtcat ccaatagtgg ggaaacactg
     7141 aaacagcttt gcctgagtgg caacgggatt gcgtgtttgt ccgactacat gatcgacaaa
     7201 gaaatcgctc gcggagaatt ggtggagtta atggcagata aagtgttgcc agtggaaatg
     7261 ccattcagtg ccgtctatta cagcgaccgt gcggtaagta cgcgcatccg ggcttttatc
     7321 gatttcctta gcgagcatgt aaaaacagct cccggaggag ctgtcagaga ggcttaatcc
     7381 cattcaggag ccagaccttc cgggctaacc aggcggtcgt tgcaatccag tgcggcgatc
     7441 gcttttttat cttcggcatc aagctgtaaa ttttgtgcct taagattact ttccaggttt
     7501 ttacgtttag tagaagaagg aattactgag taaccttccc ccatagccca cgccagaatc
     7561 acttgtgccg gagtcgcatt gtgtttagct gcgatacgag caataacctc atctttcagg
     7621 gccttaccat acgccagcgt catataggaa gtaatatgga tgccgtgctg tttagcccag
     7681 gcaaccactt tacggttttg cagataagga gagagttcaa tctggttagt agcgatgttt
     7741 tcagcaccaa cagcagcaat cgctttttcc atcaacggga tcgtgaagtt ggaaataccg
     7801 atctcacgcg tcagcccttg ttttttggct tccagcagcg cctgcataaa ctcttcaaca
     7861 gagacttcat cgtttggtga cggccagtgg attagcgtca gatcaacata atcggtacgc
     7921 aatttttgca ggctctcttt cagacttggg atcaatttgt ctttgctgag attttcaatc
     7981 cagattttag tggtgatgta gagttcatga cgtggcacgc cactttctgc aatcgcctga
     8041 cctactgcgg cttcgttatc atagatttgt gcggtatcaa ttgcgcgata accaagttca
     8101 agcgccgtta tcacagatga aataacaacg tcgtctttca gacggaaagt acctaaacca
     8161 aatgcaggga tagccataat atgcctcttt taatgctatt ttcgttaact gaggtgatta
     8221 tgggcttcag actgttgagg aaaaagagcg taaaatgcag aggatttttg cgattctggc
     8281 aataatagat atacagacga aaaaaaacct cgagcgttaa ctcgaggctt cttaata
//
LOCUS       DBANUU010000169         8325 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000169 DBANUU010000000
VERSION     DBANUU010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.171"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            10..1695
                     /locus_tag="ACIE68_004101"
     CDS             10..1695
                     /locus_tag="ACIE68_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFX5910329.1"
                     /translation="MNTALSIIDDANSNTAIDYRQEMNVIHEIVAECEKEIAFMYQVH
                     DFVYGDERHNMINRLLRLNHRPDEDRSRLNRGWLDKVDLEWVKQNIWAEYWRKVTDMT
                     NVLLIMPASRRDEWREQFIEGKQEVIKTDRTGYQMKVKEFVGVPEFKAETVIPTMLNL
                     LNDRHKYLSERVYGLFKALSPAHKTNKTNGFSERLIIADCISDFWRDSVSVNYRKEDY
                     IDDLRVLLHFFAHKEFITINRTAEVLSAAYRANDCQTGDWMNVDGNLMRVKMFKNGNV
                     HFEIHPDVAWKLNEVLAYSMPAAIPAPCRTAPKTRAPKQFGLIQKTISVPVRTALRDG
                     RLSKDKGVWYFSDSALQKSQVEELERTLSFIGGVQEKKHWQFPYEIGHTLNTIVATGL
                     IPDTKSHQFYPTPRLIAEYVARATELKSGEKLLEPQAGRGDLLAYINADLEDVTCIEI
                     APLFADILRGKGYTNTICCDFIKWSEDNVGYQFDKIVMNPPYSLGRHREHTLAALGHL
                     KVGGRLVAVLPGTAPILDWMTMDNYVYARGKSFTNEFEDTGITVSVYVFKRVK"
     gene            1757..2461
                     /locus_tag="ACIE68_004102"
     CDS             1757..2461
                     /locus_tag="ACIE68_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910330.1"
                     /translation="MNNLQLEHFNVTGHSDFPFKFTLKGYAEDAVGQIIIDKGIVKFE
                     GDFDESAKTFIDFVAKRWSEQWKNLEKRANEFDRFMDAMDTAKEALDAGTPLDLESLF
                     NGEVASAMFATMFAGEFVRSGAKNYLELDYNVPAIGDFVVTIQRKEGKTPGERVAELE
                     AVVDQRNGECDRLINELHALREERICAGSNTRNAADIYFQLVEECQIPPGGSLVDYVR
                     HLMAEVNSSHKDGEVR"
     gene            2461..2841
                     /locus_tag="ACIE68_004103"
     CDS             2461..2841
                     /locus_tag="ACIE68_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910331.1"
                     /translation="MFGIDAQRIAAFAKSPLDNPLSRSEQMELARLFLHIQKQADIFN
                     NMPNQPILDGHIQMVINSHEKGWAAIVPCTITYKLAKEVQEFRKASVESESTKAAINT
                     LIRMGFTWTGGAYWQAPHPILSGN"
     gene            2961..3602
                     /locus_tag="ACIE68_004104"
     CDS             2961..3602
                     /locus_tag="ACIE68_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFX5910332.1"
                     /translation="MSSISEAHMFAAGAHGGVGQKRKYTGEDYINHPVAVREIVALHD
                     GTVEMQIAALLHDVVEDTHVTIEMVRDHFGERVAEMVQALTNIARPEDGNRIQRFIIN
                     VRELEQNLDMQTRMIKLADLLDNTSSIVSRDPEFSAVYLAEKELMLDVLFHGKEIGAN
                     ADVVEYMERISIEHPLLLSAMAKVTEGIALLKPVHIKRYEKHKSLIWSAWEAA"
     gene            3599..4141
                     /locus_tag="ACIE68_004105"
     CDS             3599..4141
                     /locus_tag="ACIE68_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910333.1"
                     /translation="MKVEKIDVLSFVLTDLERLDPVRVMIENYEPGKGRITIICYGKA
                     WTAAWFAMGGDDVQTFIKRVSNEYLIGYFDSQLRSTVDDDNDANLLFVKSEIIKLRRE
                     REIDAVLAREMWDEAENTDDVKESCCCFGVGNKLLNLFGDDPWYADWPTVPNPKYQYL
                     ERIVNAVREGLKELDKLEAS"
     gene            4141..4641
                     /locus_tag="ACIE68_004106"
     CDS             4141..4641
                     /locus_tag="ACIE68_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910334.1"
                     /translation="MREKIKNPVVVLYKRETSDSYAVSITDGSQNMHDGLLMASVSPD
                     DSDYPFATFAMVGYYMAAEIEKLRAQRDALAAENAALKESERAFDAMCAEEHGDNWVS
                     ELTETPATDAFLAEVRAQGVDMARNAMIDFVDGEVGPNKNVPGLIRGAEICVSIAEQL
                     RKGVIQ"
     gene            4638..4985
                     /locus_tag="ACIE68_004107"
                     /pseudo
     CDS             4638..4985
                     /locus_tag="ACIE68_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            5036..5359
                     /locus_tag="ACIE68_004108"
     CDS             5036..5359
                     /locus_tag="ACIE68_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HFX5910335.1"
                     /translation="MTTSHSAITQEKAFNILERLETLATEEEISPEKLVEFSRVILRR
                     KNDMERLTSGAPSLSVRRTLCCSFCNKSQYAVKKLIAGDAVFICDECVDVCNRIIRGE
                     KERSA"
     gene            5356..5886
                     /locus_tag="ACIE68_004109"
     CDS             5356..5886
                     /locus_tag="ACIE68_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910336.1"
                     /translation="MKLSKFSELVNRILSNNHSHRRDMDVTIVVHSPGRIGSTPSVEV
                     QSIQAGFDWDAGQVMIFPSQPLTTLTPEQVADIADSVRKGQSWHAYQEYKKHKEQLEK
                     LSMELEAAKQREKDLFMENVRLKSGIAGLIHLGIRYADVEVMKIAGDAQLSTPCTDSI
                     INSIAAGIFTKEGAAR"
     gene            5883..6068
                     /locus_tag="ACIE68_004110"
     CDS             5883..6068
                     /locus_tag="ACIE68_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910337.1"
                     /translation="MRTLEVRAEDVIPGDVLITSKGQQCAVKSFWMENDKVTLFGTDG
                     SETDYDYDELLVVERAA"
     gene            6233..6481
                     /locus_tag="ACIE68_004111"
                     /pseudo
     CDS             6233..6481
                     /locus_tag="ACIE68_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            6500..6721
                     /locus_tag="ACIE68_004112"
     CDS             6500..6721
                     /locus_tag="ACIE68_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020805071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910338.1"
                     /translation="MNNAELFQKISALATECHAIASELDVGDERTEMFEIYSVLRNLC
                     RRGYATQVGRMTNPLLSSCDEDDSDEDDE"
     gene            6718..7101
                     /locus_tag="ACIE68_004113"
     CDS             6718..7101
                     /locus_tag="ACIE68_004113"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910339.1"
                     /translation="MMHKSVAGEFQKEVDNTTDLLDDILSILALLEAGDWSEHCTKTE
                     LGGRLEREITRLISDAQNDLSPEHLPCDVVLVPGMMIHEGVRTKTLLTALQRRAELSS
                     RIKLMPSKIFDVVKFTPSQHTERDK"
     gene            7098..7724
                     /locus_tag="ACIE68_004114"
     CDS             7098..7724
                     /locus_tag="ACIE68_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910340.1"
                     /translation="MSTVAKDLTEDILNEIIAGANTSLEQLLALALRAERRDRNCFYE
                     RLPGKRPDRSDEEGCDIDYMEPSEIYQLGKDDGWNAYHDAVMKLDEKVKPLSRPVDHG
                     FRDNCECSSCQTTARICSELTDKSSLIYEVNVGGNTWVECTRAAYVRAKDKGELTRVV
                     TNHPNNELKDHQIRELVNELRDIAVQYHGTQQLREKIARAVNNSVRKK"
BASE COUNT         2380 a         1790 c         2130 g         2025 t
ORIGIN      
        1 ggtgcaatta tgaatacagc cctttccatc atcgacgatg ccaactcaaa cactgctatc
       61 gactatcggc aggaaatgaa cgtcatccac gaaattgtgg ccgagtgcga gaaagagatc
      121 gccttcatgt atcaggtaca cgacttcgtt tatggcgacg aacgccacaa catgattaat
      181 cgcctgctga gactaaacca tcgaccagat gaagatcgct cgcgtttaaa tcgaggttgg
      241 ttggataaag tcgatctgga atgggtgaaa cagaatattt gggccgagta ctggaggaag
      301 gtcacggaca tgactaacgt tttgctgatc atgccagctt cccgtcgaga tgagtggcgc
      361 gagcagttta tagagggcaa acaggaagtc atcaaaactg acagaaccgg ctaccagatg
      421 aaggttaaag agttcgttgg tgtaccggag ttcaaagcag aaacggtcat acccacgatg
      481 cttaatttgc tgaatgacag gcacaaatat ctctctgagc gcgtgtatgg cttgtttaag
      541 gcgctgagtc ctgcgcacaa gacaaataag acaaacggtt tcagcgaacg tctgataatc
      601 gctgactgca tttctgattt ctggcgggac agcgttagtg tgaactatcg caaagaggac
      661 tatatcgacg atctgcgtgt cttgcttcat ttcttcgcgc acaaagaatt tattaccatc
      721 aaccgcactg ctgaggtgct atcagctgcg tatcgggcaa acgactgcca gaccggtgac
      781 tggatgaacg tcgatggaaa tctaatgcgc gtgaagatgt tcaagaacgg caacgttcac
      841 tttgaaatac atcctgacgt ggcctggaag ttgaatgagg tgctggctta cagtatgcct
      901 gctgcaatcc ccgcgccatg tcgcaccgcg ccaaaaacac gggcaccaaa gcagttcggg
      961 ttaatccaga agacgatctc cgtgccggtt cgcactgcgc ttcgtgacgg gcgattaagc
     1021 aaagacaaag gcgtatggta cttctctgat tcagctctcc agaagtcgca ggtggaagag
     1081 cttgagcgca cactgagctt cattggcggc gtgcaggaga aaaagcactg gcagttcccg
     1141 tatgagatcg gccatacgct aaatacgatt gtggctaccg gattaatacc ggatacaaaa
     1201 tcacaccagt tctaccctac cccacgcttg attgctgagt acgttgccag agccactgaa
     1261 ttgaagtctg gtgagaagct gctggagcct caagccggac gtggggatct tctggcctat
     1321 atcaacgccg atctggaaga tgttacctgc atagaaatcg cacctctctt cgctgatatc
     1381 ctgcgcggaa aagggtatac gaacacgatt tgctgcgact tcataaagtg gtctgaggac
     1441 aacgtaggtt atcagttcga caaaatcgtt atgaacccgc cgtactcgct tggtcgtcat
     1501 agagagcaca cgctggctgc gctggggcat ctgaaagtcg gcgggcgtct tgtagcagta
     1561 ttgccgggca ctgcgccaat actggactgg atgacgatgg ataattacgt ttatgccaga
     1621 gggaagtcgt ttaccaacga gtttgaagac acagggatca cagtcagcgt atacgttttc
     1681 aaacgcgtta aatgataggt aaatacttac ctaatttgtg taagaatgta gttactaaac
     1741 gataagagaa aaacacatga acaacctcca gttagagcat tttaacgtca caggccattc
     1801 tgattttcct ttcaagttta cattgaaagg ttatgcagag gatgcggtag gccagatcat
     1861 tattgataaa ggcatcgtta agtttgaggg ggattttgat gaatccgcga aaacattcat
     1921 agacttcgtt gccaaacgtt ggagcgagca atggaaaaac ctggaaaagc gcgccaatga
     1981 gtttgatcgg ttcatggatg caatggatac agcaaaagag gctcttgatg ccgggactcc
     2041 gttagatctg gagtcacttt tcaacggcga agtggcctct gcgatgttcg ccaccatgtt
     2101 cgcgggtgag ttcgtccgca gcggtgccaa aaactacctt gagctggatt acaacgtccc
     2161 tgcaattggc gatttcgtcg ttaccatcca acgcaaagaa ggtaagacgc caggtgaacg
     2221 cgtcgcagag cttgaggccg ttgtggatca gcgtaacgga gagtgtgacc gtttgatcaa
     2281 cgagcttcat gcacttcggg aagaaagaat atgtgcgggt agcaatacac gtaatgcagc
     2341 ggatatctac ttccagttag ttgaggaatg ccagattcca ccaggtggct ctcttgtcga
     2401 ttacgtcaga catttaatgg cggaagttaa ctccagccat aaagatggtg aggtgcgctg
     2461 atgtttggca ttgacgcaca gcgtatagcc gcttttgcaa aaagccctct tgataatccc
     2521 ttgtctcgta gtgagcaaat ggagctggca aggctttttc ttcacattca aaaacaggca
     2581 gacattttca ataacatgcc taatcaacct attctggatg gtcacatcca gatggtcatt
     2641 aacagtcatg agaaaggctg ggctgcaatc gtaccctgca caattacata caagttggcg
     2701 aaagaggttc aagagtttcg aaaagccagt gtcgaatcgg agtctaccaa agccgcaata
     2761 aacactctta ttcgcatggg gttcacatgg accggtggag cctactggca agcacctcac
     2821 cccattttat ccggcaatta gacgctacaa atccttcggc atgaataaat aaaggccata
     2881 agttgtggtc ttaaataaat tgttttctac cttttcttat ttgtgaaaat aaccacaact
     2941 agaaaacaac ggagttaaga atgtctagta tttccgaagc tcatatgttc gccgctggcg
     3001 ctcatggtgg cgttggtcag aaaagaaaat ataccggcga agactacatc aaccacccgg
     3061 ttgctgtccg agagattgtt gcattgcatg acggaaccgt ggagatgcaa atcgcggcgc
     3121 ttctgcatga tgtggtcgaa gacacccatg taaccataga gatggttcgt gatcacttcg
     3181 gtgagcgcgt agctgaaatg gttcaggctc tgaccaacat tgcaaggcca gaagacggca
     3241 atcgaataca gcgtttcatc atcaatgtcc gggagctgga gcaaaatctc gacatgcaga
     3301 cgcgcatgat caagctggct gacctgctcg acaatacctc ttctatcgtg agtcgcgatc
     3361 ccgagttttc agctgtctac ctcgcagaga aagaactgat gctggatgta ctctttcacg
     3421 gcaaagagat tggtgccaac gctgatgttg tcgagtacat ggagaggata agtatagaac
     3481 acccgttgct attaagcgca atggcgaaag tcactgaagg tattgcgctt ctgaaaccgg
     3541 tacacatcaa acgttatgaa aaacacaaat cgctgatctg gagcgcgtgg gaggctgcat
     3601 gaaggtcgaa aaaatagatg ttctttcctt tgtgctgacg gatctggaac gcctcgatcc
     3661 ggttcgagtg atgattgaaa actacgaacc tggtaaggga agaatcacca tcatctgcta
     3721 cggaaaagcg tggactgcgg cttggtttgc tatgggcggt gatgatgtgc agacgttcat
     3781 taagcgggtc agcaacgagt atctaatcgg ctatttcgac tctcaactgc gaagcacggt
     3841 cgacgatgac aacgatgcaa atctgctttt cgtgaagtca gaaatcataa agttacgaag
     3901 agagagagaa atcgacgccg tactggctcg tgaaatgtgg gacgaggcgg aaaacaccga
     3961 tgacgtaaaa gaaagctgct gttgtttcgg cgtcggtaac aaactgctga atctctttgg
     4021 tgatgatccg tggtatgccg actggccaac ggtaccaaac ccgaaatacc agtatctgga
     4081 acgcattgtg aacgcggtac gtgaagggct gaaagagtta gataagttag aggcatcgta
     4141 atgagagaga aaatcaagaa cccggtcgtc gtattgtata aacgggaaac cagcgattct
     4201 tatgcggtat ccatcactga tggaagccag aacatgcacg atggtctgct aatggcctcc
     4261 gtatctcctg atgattctga ctaccctttc gccactttcg ctatggttgg ttactacatg
     4321 gctgccgaaa ttgagaagtt gcgagcgcag agagacgcat tagcggcgga gaatgcagcc
     4381 ctgaaagaat ctgagcgagc attcgatgcg atgtgtgccg aggaacacgg agataattgg
     4441 gttagcgaat taacggagac tccagccacc gatgcttttc tggctgaagt acgtgcgcag
     4501 ggcgtggata tggctcgtaa cgcgatgatt gattttgttg atggtgaagt tgggccaaac
     4561 aagaacgttc cggggctgat tagaggcgca gagatatgcg taagtattgc tgaacagctt
     4621 cgtaaaggag tcatccagtg agcaagattg actatcaggc actgcgtgag gcggcagagc
     4681 gtgcaattcc ggcaatggaa cgcctgttaa tgttgccagt tgatgatgat ctgatatgcg
     4741 aacaggaact taaagatagc ggtgttgata ttgatgccct caacgccttc aaatttctgg
     4801 ccggaccaga aaccgtgctg gcactactgg atgagatcaa cgcattagag gaaacgcgta
     4861 tcaacgatgt ttgccgtatt gcggaactaa caaaacaact ggagttggca aaatcaaaac
     4921 tcaacgagca gcgtgagtat tacgagggcg ttatctcgga tggaagtaag cgcatagccg
     4981 ctcttcttcg caaggacaat cttgccagcg ccacgaacat tgaaggtgaa agaaaatgac
     5041 cacatcgcac tctgctatta cccaagaaaa agccttcaac atactcgaac gactagagac
     5101 gctcgctacg gaggaggaga tatccccgga gaaactggtt gagttcagcc gtgtgatatt
     5161 gcgtcgcaag aacgatatgg agcggctgac atctggcgct ccatccttat cagtcaggcg
     5221 aacactttgt tgcagcttct gcaacaaatc ccagtacgcc gtcaaaaagt taattgctgg
     5281 ggacgccgtt ttcatctgcg acgagtgtgt ggatgtgtgc aacagaatta tccggggaga
     5341 gaaagagaga tcagcatgaa attatccaaa ttttctgagt tggtgaatcg tattttgtcc
     5401 aacaaccaca gccatcgtcg cgatatggat gtaacgatcg ttgttcattc gcctggtcgc
     5461 atcggttcaa caccatcagt tgaggttcag tcaattcagg cgggttttga ttgggatgcc
     5521 gggcaagtga tgatttttcc atcacagcca ctgaccacgc taacaccaga gcaggttgct
     5581 gatatcgctg atagtgtgcg caaaggtcag tcttggcacg cgtatcagga atacaagaag
     5641 cataaagagc agttggaaaa attgtcgatg gagttggaag ccgctaaaca gcgggaaaaa
     5701 gatctgttta tggaaaatgt tcgacttaag tcaggtatag ccggtctgat acacctcggt
     5761 attcgatatg cggatgttga ggtcatgaaa attgctggag atgcccagct ttctactcca
     5821 tgcactgaca gcatcataaa cagcattgca gcaggcattt tcaccaaaga gggggcagca
     5881 cgatgagaac actagaggtt cgcgctgaag acgtaatccc tggtgatgtg ctcataacat
     5941 ctaaaggtca acaatgtgcg gttaaatctt tttggatgga aaatgacaaa gtgactctgt
     6001 tcggtacgga tggttccgaa actgattatg actacgacga attgcttgtt gttgagagag
     6061 ctgcctaatg accaccgtta acaataagaa atgctaccca agcgagaaat atcttaatga
     6121 gctgatcacc aacattgagt ttgctgcaag ggcaccagtt gaagtcgtga gagcgattgc
     6181 agcagagcta cagaagcacc gtaaggccta tgccaataca gccgcatcta aggatggttg
     6241 gataagctgt agtgagcgaa tgcctgtaat tggcgagcta aattggagaa ctagttttcc
     6301 tttactggtt acgtgtgaga tcggcgttat acctgcttat tacggctttg tgagcgttaa
     6361 tggtgatagg cattatggct ttatggagag tcttaaatac ggagacgata acggcaacca
     6421 tcctcaaact aatgaatatg gtctgattag caatgtcaca cactggatgc cactaccaga
     6481 acgccgcagg agtcgaacag tgaataatgc agagttattt cagaaaatat cggctctcgc
     6541 gactgaatgc cacgctatag catctgagct tgatgttggc gatgaacgaa ccgagatgtt
     6601 cgaaatatac agtgtgctgc gcaatctctg tcggcgtggc tacgccactc aagtagggcg
     6661 aatgactaac ccactactct catcctgtga tgaggatgac tcggatgagg atgacgaatg
     6721 atgcataaat cagtagccgg tgagtttcag aaggaagtcg ataataccac tgatctattg
     6781 gacgatattt taagcattct cgcgctgctt gaggctggcg attggtcaga acattgcact
     6841 aaaacagagc taggcggtcg gcttgaaaga gagattacac gactgattag cgatgcccaa
     6901 aatgatttgt cacctgagca tctcccctgc gatgtagttc ttgtgccagg gatgatgata
     6961 cacgaaggcg tcagaacgaa aacattatta accgcattac agagaagagc cgaactaagc
     7021 tcgcggataa agttgatgcc atccaaaata tttgatgtcg tcaaatttac gccatcgcaa
     7081 catactgaga gagataaatg agcacggtag cgaaagatct cacagaagat attctcaatg
     7141 aaattattgc tggtgcaaat acctcgcttg agcaacttct tgcgttagca ctgagagccg
     7201 aacgtcgaga cagaaattgt ttttatgagc gattaccagg gaagcgacca gatagatcgg
     7261 atgaagaagg ttgcgatata gactacatgg agccttcaga gatttatcaa cttggtaaag
     7321 atgacggctg gaacgcttat cacgatgctg tgatgaagtt ggatgaaaaa gttaaacctc
     7381 tttctcgacc ggtagatcac ggtttccgcg ataactgcga atgctctagt tgccagacca
     7441 cggcccgtat ttgttccgaa ttgacagata agtccagcct aatctacgaa gttaatgtag
     7501 gcggtaatac atgggtcgaa tgcacaagag ctgcatacgt aagagcaaaa gacaagggtg
     7561 aattaaccag agttgttacc aatcacccaa ataatgagct taaagatcac cagattcgag
     7621 aactggtgaa cgagttgaga gatattgcgg ttcagtacca cggaacgcag caattgcggg
     7681 agaagattgc cagagcagtg aacaactcag tccgtaaaaa ataagcaata gtacgataaa
     7741 gaggccccat gctcttgatg gggcctgtag caactagcgt tatggacgct ggtttacgta
     7801 ctcaatgatc gctttgataa tcgcgaacat cggcggcacg attttgaaca gtaagttaac
     7861 cataacaggc ctcacttagt tttatcgcgc ctagctgcaa atacctttgg gcttgccttt
     7921 gcagttgcaa tatctgtagt cgccagatac ttacggcacg atttcgagtt aaggtaaggt
     7981 tttagagtca ccaaaaacct ggacttaaat cttctttgag aacaacgatg tttagtaaat
     8041 ggtcattatt acctggtatg accactcaac gtttcttgat gaatccagta cattcggtta
     8101 atcttaattc agtcgccaga tacttatggc tcaggaacag agccgcaaac tctgtttcct
     8161 tttaaaaagc ccagcctagt caactgggct ttttaatgtc tatttttaac ccaatgaacc
     8221 ttcaataccg caacatcagt gtcttgcgaa ttaagatagt gtgtataggt tatgcatgca
     8281 agtgcataac tctgtggata actcaggaag gaaaaagtag cttcc
//
LOCUS       DBANUU010000170         8315 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000170 DBANUU010000000
VERSION     DBANUU010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.172"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..266
                     /locus_tag="ACIE68_004115"
     CDS             <1..266
                     /locus_tag="ACIE68_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Shufflon protein B"
                     /protein_id="HFX5910341.1"
                     /translation="PTCQSGTWKSSSASIWTNIKTFTLYPKNTQVLGRFKLCINTYRI
                     DGREMAETEVVPIDMPDSNGEMTWQAKNYTQYSSYFMKITCLK"
     gene            584..1738
                     /locus_tag="ACIE68_004116"
     CDS             584..1738
                     /locus_tag="ACIE68_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFX5910342.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPI
                     ALRNMDEITTVDIATYRDVRLAEINPRTGKPITGNTVRLELALLSSLFNIARVEWGTC
                     RTNPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEIL
                     ALRWEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKN
                     AWRIATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRA
                     WQLVSKLDARRRQTQKVAAWFVPYPAHITTIDEENGQKAHRIEIGDFDNLHVTATTKE
                     EAVHRASEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
     gene            1889..2713
                     /gene="traE"
                     /locus_tag="ACIE68_004117"
     CDS             1889..2713
                     /gene="traE"
                     /locus_tag="ACIE68_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFX5910343.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLISDSSNTEVIFWHSFPFAGNTKREYCGVILRQKKEINGSVIYPALSLS
                     PTLLNDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAGNLITGQILPVSLFNTSLLPW"
     gene            2798..4000
                     /locus_tag="ACIE68_004118"
     CDS             2798..4000
                     /locus_tag="ACIE68_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFX5910344.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPDDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDIDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGA
                     MAQLQFTF"
     gene            4060..4644
                     /locus_tag="ACIE68_004119"
     CDS             4060..4644
                     /locus_tag="ACIE68_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFX5910345.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEIN
                     ADNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNA
                     VRTIQTAKFFSGKEPVVMDSLSDCNNDLYKTLESIARESHKRNIVIMTHNHCLSFLAR
                     DRLGKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
     gene            5039..5497
                     /gene="traH"
                     /locus_tag="ACIE68_004120"
     CDS             5039..5497
                     /gene="traH"
                     /locus_tag="ACIE68_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="HFX5910346.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQ
                     YTLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVR
                     LPLPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
     gene            5494..6312
                     /gene="traI"
                     /locus_tag="ACIE68_004121"
     CDS             5494..6312
                     /gene="traI"
                     /locus_tag="ACIE68_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="HFX5910347.1"
                     /translation="MKALTSLLLPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSE
                     KPDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEG
                     WLPPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGT
                     EGSVVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQG
                     MITRPDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLIGTEGGSR"
     gene            6309..7457
                     /gene="traJ"
                     /locus_tag="ACIE68_004122"
     CDS             6309..7457
                     /gene="traJ"
                     /locus_tag="ACIE68_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFX5910348.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVS
                     VSRFGRRVRCSSATLPTTLMSSLIDELFGREVIPRVLAGNPVDRTIQINGDASGRYGL
                     KRGERIRLRSHLIQGTSGAEEKAISITMRVIPTEIPDILSMNIEPDLLEAMVCKSGLG
                     FVCGETGSGKSTLCSALYRYIMDNFPDAKIVTYEDPVEYILGNENDLLPPHQAEIGRD
                     VVSFAAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLAR
                     LLGLFPPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMP
                     HAEWGHHIDAIIRQEKRRIRDQILEMYIRNEVDRREAILFIPPGELRS"
     gene            7454..7744
                     /locus_tag="ACIE68_004123"
     CDS             7454..7744
                     /locus_tag="ACIE68_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFX5910349.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTG
                     IILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
     gene            7759..8310
                     /locus_tag="ACIE68_004124"
     CDS             7759..8310
                     /locus_tag="ACIE68_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFX5910350.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGS
                     ASALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQE
                     AMKYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNM
                     PDVAAQYLEHWQDRWNKGTDWKP"
BASE COUNT         2262 a         2032 c         1926 g         2095 t
ORIGIN      
        1 ggcctacgtg ccaatccggt acctggaaat catcatcagc gtcgatatgg acgaatataa
       61 aaacatttac gttatatccc aaaaacacac aggtcttagg gaggtttaaa ctgtgtatta
      121 acacctatcg tattgacggg cgtgaaatgg cagaaacaga agtcgttcct attgacatgc
      181 ctgactctaa cggcgagatg acttggcaag ccaaaaacta cacacagtat tcgtcctact
      241 ttatgaagat tacttgcttg aaatagctta ttggcaaatg gcatatacag ttattgaacc
      301 attctgatct tcggttgtat cgcacgcagc cgaccatgag ttattggctg atgggaatga
      361 cctggtaagc catatgtaac cagttcgtga ggtgcatatt ccgccgccac ctgtaacacg
      421 ttttccagtt ggacaagtag ctgttgctcc aggaaagcgc caacctgtgg cctgtgctgt
      481 agcaatttga agctcaccag atcctacctt tctccaggta ccggattggc acgcttgcag
      541 agaatgattg ttggcattat tttttgcctc ttttgaggtt agcatgccgt ctccacgcat
      601 ccgtaaaatg tccctgtcac gcgcactgga taagtacctg aaaacagttt ctgttcacaa
      661 gaaagggcat caacaggagt tttaccggag caatgttatc aagcgatatc ccattgccct
      721 tcggaatatg gacgaaataa caaccgttga tattgctaca tacagagacg ttcgtttagc
      781 agaaatcaac ccccgaacgg gtaaacccat tacaggtaat actgtacgtc ttgaactcgc
      841 ccttctgtca tctctgttca atattgctcg tgttgaatgg ggaacctgtc gtactaaccc
      901 ggttgaactg gttcgcaagc cgaaagtatc ctcgggacga gatcgccggc taacgtcttc
      961 agaagaacgt cgcctttctc gctatttccg cgaaaaaaat ctgatgttgt atgtcatttt
     1021 ccatcttgcc cttgagacag ccatgcggca gggcgaaata ctggccttac gttgggagca
     1081 cattgatttg cgccacggtg tggctcattt acctgaaacc aaaaacggtc actcacggga
     1141 tgttcctctg tccagacgtg cccgtaactt tcttcagatg atgcccgtta atctccacgg
     1201 caatgttttt gattacaccg catccggctt taaaaatgcc tggagaatag ccacacaacg
     1261 acttcgcatc gaggacctgc attttcacga tctacggcat gaagcaataa gccgcttctt
     1321 cgaactgggt agcctgaatg taatggagat tgctgcaata tcaggacatc gttccatgaa
     1381 tatgctgaaa cggtatactc atcttcgtgc atggcaactg gtcagtaagc ttgatgcccg
     1441 ccggcggcag acacaaaaag tggcagcatg gtttgtgccg tatcctgccc atatcacgac
     1501 catcgatgaa gaaaatgggc agaaagcgca tcgtattgag atcggtgatt ttgataacct
     1561 tcacgtcact gccacaacga aagaggaagc agttcaccgc gccagtgagg ttttgttgcg
     1621 tacactggcc attgcagcac agaaaggcga acgtgtccca tctcccggag cgttacctgt
     1681 taacgaccct gactacatta tgatttgccc tctgaacccg ggcagcaccc cgctgtaaac
     1741 atcattcaaa acacatacag aaaggcgagt tgcaacaact cgccaaacac attcgatctg
     1801 catgatcgaa attattcgtt tcaccagaaa aaacaccgaa gatacaataa ccacatgagc
     1861 tgaaaagact ttttcagtcg gaggaacaat gagactgaat acgaccggta ttgcggcccg
     1921 aatgatgctc tcgctggaca aaaaagcaat cggcgaaaaa ctcatcaacg gtcgtcagat
     1981 aaacccgaca cctctgcagg tccgttaccc ccaaggggta agagaagttc tggggataat
     2041 gagcgaacaa ttagccatat ccaccgctga tttaacccga atcctgcttg aggatgctct
     2101 tcataatatg tttatgcctg cggataacac cacaggaaac ataataagca gaattgaata
     2161 catcatgctg tcgcatgata taaatgcgcc attactggca acattgctgt cgccatggaa
     2221 tatccgctcc acggttatac aggacccggc cagacttgca gactatcttt cagctgacgc
     2281 tctcgaacat cttgctgaat gttttaatct caatccggac tggctgaatg gccatgaaaa
     2341 ctatccgata gcgttgtccg gtgaatggcc agatactgct gataatttca gaatgcttat
     2401 tagtgatagc agcaacactg aagttatttt ctggcatagt ttccccttcg caggaaacac
     2461 gaaaagagaa tattgcgggg taatattgag acagaaaaaa gaaataaatg gctctgtaat
     2521 atatccggca ctatcattat caccaacact tctgaacgat gaaaaaagaa aatggttaac
     2581 tgaatataca accaggcaga atacaacaat gtctttgcga cgcgtcacgt tgagacctgg
     2641 ccttgccggg aaccttatta cagggcaaat actacctgtt agcttattta atacgtcttt
     2701 attaccctgg taaataccag atagtaccaa agtaaaaatt acggcacatc cgtgtcatag
     2761 cgttgttttg tctaaattct gataaacagg aaattaaatg atgaagaaaa atcatattac
     2821 ccgcaccatc attgcatctg ctgtattatt ttcctttaac gctgcagcag cgacgtcata
     2881 ttttgaagcg cgtaacgatg caatgggtgg taccggggta gcatcttccc attatggtgt
     2941 ggcacctctg gcaaacccgg ctttactgac aaagcacaac agcaatgatg atttcagcct
     3001 tcttctgccg tctgttgggg cacaagttgc tgatcctgat gatgtatcaa ataaagctga
     3061 tgatgttaaa gatgactggg atttgtttga cagtgctgta gataaccagc atggcgtaca
     3121 acaggcagcg gcaaatctta aacatcgtct gcaggaattc cgcaatatca atgcagatgc
     3181 tcaggtaggt gtatcagctg tagcagccat ggcaaatgac actctgccgt ttgcccttat
     3241 ggtcaagtct tacggaactg tcagcgtaaa cggaaaagta aacgatgcag acctggatta
     3301 tctggacaaa gttgccaatg gaacaattac cgacgtcgat aagaatgccc tgacctcacg
     3361 cgcattcggg cgtgcagctg taattaccga cgtcggaatt tcatttgcca aagaactgga
     3421 aacggccgga caaaaatggt ctcttggcgt cacaccaaaa tatcagcgtg tggacctgtt
     3481 taactacaac gtaacggttc gtgattacga caaagacgat tttgacggtg acaagtacca
     3541 caacacgaaa aacggattca acgccgatat tggtgcttat acagacctga atgacaactg
     3601 gactgtcggt cttgttgccc agaacatcat tccacgcagt attgatacta aagtcgtcaa
     3661 tggctttaaa gaaaccttca aagttcgtcc gcaggcaacc gccggtgtat cctggcataa
     3721 tgacctgttt accactgctc ttgacattga tctgactcct gccagtggct tcacttctga
     3781 cagcaaacgt cagtttgcaa gtgttggtgc tgaatttaac gcatggaaat gggcgcaatt
     3841 gcgtgccggt tatcgtcaga acatggcatc aaactccgga agcgccttca ccgctggcgt
     3901 tggtatctct ccatttgacg tcgtacatat cgatgtttcc ggcctggtag gaactgacca
     3961 cgattacggt gcaatggcac aacttcagtt cactttctga ttactgtccc cgcccccggc
     4021 actaaacccg ggggtatttt tatattcagt ttaaataaca tgatgaaacc acgttcttct
     4081 tattcaaaaa cagcattcat tcttttattt tctgttttcc tggtagcggc agtgacaaaa
     4141 gcaaaaagct cgcttcccga tatcaccctg gaacaggcaa aagaaattaa tgctgataac
     4201 accgttattt tccttttccg ccatggagaa cgttgcgacc gttcagatat gccatgctat
     4261 tcggataaaa gcggaatcac tattaccggt acagagaaag cacagcagga aggtattaag
     4321 ttcgcaacaa tcttcagtga atatgatatt tactccagca atgcggtaag aaccatacaa
     4381 actgcaaaat tcttctccgg taaagaaccc gtcgttatgg acagtttatc agactgcaat
     4441 aatgatcttt ataagacgct tgaatcaatc gcaagagaat ctcataaaag gaacatagta
     4501 ataatgacac ataatcattg tttatcattt ctggccagag ataggctcgg caagaaattc
     4561 aaaccggcgt accttgatgc cctgatcatg cattacgatg gtacccggct catacttgac
     4621 ggaaaataca acaaagaggc atagaggata tttgaattta cataaattct gatatactca
     4681 gtaagataca agagtgtacg ggcacatgtg cctgtctgaa ttgcaggtta tccggacgag
     4741 ggccgggaac tgtttcaggg acaagcgccg gtacgttcga ttcataaatc gtcgagtcat
     4801 ccatcatgga tcgattcggc gcggagcgtt aaagattacg ctcatattcg ttttatgtct
     4861 gacgacacat caaccagcca gtatcaggaa taatcacctt cagtaaacta tgaggctatt
     4921 tacctggcca cactggcatt tatttccggt aacgcaagta tagccggaaa caaacatcat
     4981 cctgtctgta ccggagcacc ggtatatccc attctgattt aactgctcag gaattattat
     5041 gaaccgtatt atacctgcgg ggtgtcttgc cgcccttttt ctggctggct gcaactcccc
     5101 ttctcagcat actcctgttc cgcgtaacta cggtgctgcc tctgatgtgg cggttacccg
     5161 gcacactcag tacacattat gggacaaagg tgtattttcc ggccaacttc cggtttcaac
     5221 ggggtctcat cttctgacag ctaacagcga tcgcctttcc tttgactggg aaggtgatgc
     5281 aattgaatta ctgaacgaac tggccagagt cagggggatg cagtttaact acaatggtgt
     5341 ccgcctgccg ttaccagtca accttcacgt ccgggatatg acgttttcca atacgttgag
     5401 acttattgaa gcccagacag cctggagagc aacaatccat caatatccgg gcttgttaca
     5461 ggtcagcttc atgcagccgg agaaccggaa aaaatgaaag cactcacctc tctccttttg
     5521 ccagtaatac tgcttactgg ttgttcttct tcccctacca tctcgccagg tggtgtcaat
     5581 acgccaccac ctgacattaa tgcatggctt aaaccacgaa gtgaaaagcc ggatagtctg
     5641 tcagaaacac gctggaaaat gctgacagac gcaggtaaaa cgcttggatt tcgtggtggc
     5701 aaggctcagc gttcatggga gctgattcag gcgctcaacg cccgggaaag cacacttaat
     5761 gccttatacg atttccggcc actgattagc ccggaaggct ggctaccacc ggttattgat
     5821 gaagcacagg acgtagctca tatcacgcca gatcaaatcc ggacctcttc ccgggtctgg
     5881 accatcattc gccctgaacg ctttgtcagc aatccaccgg gctggcgtga ctggcttctc
     5941 cgggggcttt caacaacagc cactccgggt acagaaggca gcgtcgttcc agaagacagc
     6001 gtgcaacgaa aagtgtggga aaccgcatta cggcagggat ggcaggaagg aagacaaaac
     6061 gccgacctga cgcttgaagc caaccagaaa accctcaccc gtgattaccg gggcatgatg
     6121 ctgtactcac ttctctggcg tcagggcatg atcacccgtc cggatgtttc tgatcaaatg
     6181 caaaccgtta caggtgacgg gaaaaaactg gtcactggag atcgtgttcg tcgccttaaa
     6241 aatcatgcgg aattcaatct tcagaaatct cactggcgtc cgcttatagg cactgaagga
     6301 ggctcccgat gaatattcct gatgaatttg gtctttttcc gtttcagcgt ttcactgcag
     6361 atgaactacg gcatttcttt gtctggtgtg ccgcacataa agtcagtgat gtggatctga
     6421 ccggcgggtc acctgtatct gtcagtcgct ttggccgacg ggtgcgttgt tccagtgcaa
     6481 cgttaccaac cacactcatg tcctccctga ttgatgaact gttcggtcgg gaagtcattc
     6541 cccgcgttct ggcaggcaat ccggttgaca ggacgataca gattaacggg gatgcatccg
     6601 ggcgctatgg tcttaaacgc ggagaacgca tccggttacg ctctcacctc attcagggca
     6661 catctggtgc ggaggagaaa gcaatctcaa taaccatgcg tgtcatcccg acagaaatac
     6721 cggacattct ctccatgaac attgaaccag acctgcttga agcaatggtc tgcaaaagcg
     6781 gtctgggatt tgtatgtggt gaaaccgggt caggaaaatc aacgctgtgt tcagcactgt
     6841 accgttacat tatggacaat ttccccgacg ccaaaatcgt gacatatgaa gacccggtgg
     6901 aatatatcct gggaaatgaa aacgatctgc tcccaccgca tcaggcagaa atcgggcgtg
     6961 atgtggtcag ttttgctgcc ggactgcgtt ctgctgtacg acgtaatccc gaaattatcg
     7021 gtgttggtga gatcagggat aacgaaaccg ccgacgcagc ggtacaggca ggcaacaccg
     7081 ggcattactg cctgagcacc atgcatacga aatctcctgg tgaaacgctg gcacgtcttc
     7141 ttgggctgtt tccccctgtc atcagggatt cgatggcctg ggctgtcctg tcactgttgc
     7201 agttcattct ggttcaggtt ctggtcagaa caaatgacgg tggtcgtaaa gccgtccggg
     7261 aatatatcgt cattaacgat gagcttcgcg acaatctttc cggaatgccg catgcagagt
     7321 gggggcatca cattgatgcc ataatccggc aggagaagcg ccgtatccgg gaccagattc
     7381 tggaaatgta tatccggaat gaagttgacc gaagagaagc gattcttttt atcccgccag
     7441 gggagttacg gtcatgaaaa gagaaggttt ctggcgatat gcaacaccgc cagtcaacat
     7501 ccagggcgta cctctgccgt tcaccatgat ttatctgttt atatgcccct ttccctccaa
     7561 aacaaccttc tggatttgca ccggcatcat tttatttttc gttattctcg accgttcagg
     7621 ctggactgtc aggactatct gtacacgcat tttcagcatt ctgagagggg ccatcgcctc
     7681 cggtcgtcca tggtggtatc gccaccatac ggaatcacca cgagactgga ctggcctcga
     7741 ttaaattctg gagtctttat gtcaggtaaa aaaactatcg ttactctgct ccgggtatcg
     7801 ctactggcat gcccgttact gttcacgaca ccatcgtttg caatgatcga cacgccatcc
     7861 gtgaaggtcg gcttttctcc tgaaggaagc gcatccgctc tggtgcttga cacaatcaac
     7921 agtgcggaaa gctccatcag gatgatggca tattccttca ccgacccgga tgtaatgcat
     7981 gcgctggcaa aagcgaaaaa acgcggagtg gacgtccgta ttgttgttga tgacaagggg
     8041 aatacgaacc gggcaagtca ggaagcgatg aaatatatca acctgctgga cattcctctc
     8101 cggactgtag atgccttccc catccatcac gacaaagtca tcattgttga cggaaacacg
     8161 gttgaaactg gctcctataa cttctctcgt gctgctgcac gcaagaattc agagaatgtc
     8221 gttgtgctca agaatatgcc ggatgtagcc gcacaatatc ttgaacactg gcaggatcgc
     8281 tggaataagg gtacagactg gaaaccctga caaac
//
LOCUS       DBANUU010000171         8229 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000171 DBANUU010000000
VERSION     DBANUU010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.173"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            4..1728
                     /gene="iucA"
                     /locus_tag="ACIE68_004125"
     CDS             4..1728
                     /gene="iucA"
                     /locus_tag="ACIE68_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFX5910351.1"
                     /translation="MILPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPAHLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESYAHTQQTIDARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RQEAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQVWCQALFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSVRTLSVKEVERGMRLARLAQTDGWQMLQARFPTFRVMQEDGWAGLRDL
                     NGNIMQESLFSLRENLLLEQPQSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATEWLDTIDEAQAENIFTREQLLRYFPYYLLVNSTFAVTAALGAAGLDSEANLM
                     ARVRTLLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            1729..2676
                     /gene="iucB"
                     /locus_tag="ACIE68_004126"
     CDS             1729..2676
                     /gene="iucB"
                     /locus_tag="ACIE68_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFX5910352.1"
                     /translation="MSGANIVHSGYGLRCEKLDKPLNLGWGLDNSAVLHWPGELPTGW
                     LCDALDQIFIAAPQLSAVVLPWSEWCEEPQALTLFGQVQSDIIHRSAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQIAYLERQLTSKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGVTHYLLLNEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            2676..4418
                     /gene="iucC"
                     /locus_tag="ACIE68_004127"
     CDS             2676..4418
                     /gene="iucC"
                     /locus_tag="ACIE68_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFX5910353.1"
                     /translation="MNHKDWDLVNRRLVAKMLSELEYEQVFHAESQGDDRYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLNADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNEMDIHQLLTAAMDPQEFARFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            4415..5748
                     /gene="iucD"
                     /locus_tag="ACIE68_004128"
                     /pseudo
     CDS             4415..5748
                     /gene="iucD"
                     /locus_tag="ACIE68_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016528830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
     gene            5774..7975
                     /gene="iutA"
                     /locus_tag="ACIE68_004129"
     CDS             5774..7975
                     /gene="iutA"
                     /locus_tag="ACIE68_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFX5910354.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAVFLQSGYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         1928 a         2162 c         2185 g         1954 t
ORIGIN      
        1 attatgatcc tgccctctga aaaatccgcc acagatgtgg ctgcgcagtg cttcctgaat
       61 gccttgattc gtgaaaccaa agactggcaa ctggcggaat acccgccaga cgaattgatt
      121 atcccgctgg acgaacagaa gtcgctccat ttcagagtgg cttatttctc cccaacccag
      181 catcaccgct ttgcatttcc tgctcatctg gtcacggcat caggcagtta tcctgtcgac
      241 tttaccactc tctcccggct tattattgat aagctccggc atcaactttt tctgccagtt
      301 cccctctgcg aaactttcca ccaacgcgtg ctggaaagct acgcccatac gcaacagaca
      361 attgatgccc gccatgactg ggccatcctg cgtgaaaaag cgttgaattt tggcgaggct
      421 gagcaggcac tgctgacagg acacgctttc caccctgcac ctaagtctca tgaaccgttt
      481 aaccgccagg aagctgaacg atacctgcct gacatggcac ctcacttccc gctgcggtgg
      541 ttttcggtgg ataaaacgca aatcgctggt gaaagtctgc atcttaacct tcaacagcgg
      601 ttgacgcgat ttgccgcaga aaatgcgccc cagttactca acgaattaag tgacaatcaa
      661 tggctgttcc cgctgcaccc gtggcaggga gaatatcttt tgcagcaagt gtggtgccag
      721 gcactttttg ctaaaggact tatcagagac ttaggcgagg ccggcacgtc gtggctgccg
      781 accacctctt cccgctccct ctactgtgct accagccgcg atatgatcaa gttctccctg
      841 agcgttcggc tgaccaactc cgtccgtact ctgtctgtga aagaggtgga gcgaggaatg
      901 cgcctggcac gtctggcgca aaccgacggc tggcagatgc tacaggcccg cttccctact
      961 ttccgggtaa tgcaggagga cggctgggcc gggctgcgcg atcttaacgg caacatcatg
     1021 caggaaagtt tgttttccct gcgtgaaaac ctgctgctgg agcagccgca aagccagacc
     1081 aacgttctgg tttccctgac tcaggccgca ccggatggcg gtgattcgct gctggtttcg
     1141 gcggtaaaac gcctgagcga tcgcctcggt atcactgtgc aacaggccgc ccacgcatgg
     1201 gtcgatgctt actgtcagca ggtgctgaag cctctgttta cggctgaagc ggattacggc
     1261 ctggtgctgc tggcgcatca gcaaaatatt cttgtgcaga tgcttgggga tctgccggtc
     1321 ggatttattt accgtgactg ccagggcagc gcgtttatgc ctcacgcgac agagtggctc
     1381 gataccattg acgaggcgca ggcggaaaat attttcaccc gtgagcagtt gctgcgctat
     1441 ttcccttatt acctgctggt taactccact tttgccgtaa ctgctgcgct gggtgctgcc
     1501 gggctggaca gcgaagcgaa tctgatggct cgtgtacgaa cattgctggc tgaagtgcgt
     1561 gaccaggtga ctcataaaac ctgtctcaac tatgttctgg aaagtccgta ctggaacgta
     1621 aaaggtaact ttttctgtta tctgaacgat cataacgaga acaccatcgt tgacccttcg
     1681 gtgatctatt ttgatttcgc taacccgctg caggctcagg aggtctgaat gtctggggca
     1741 aacattgttc acagcggata tggactgcgc tgtgaaaaac tcgacaagcc tctgaatctt
     1801 ggctgggggc tggacaatag cgcggtgttg cactggcccg gggagctgcc aacagggtgg
     1861 ctgtgcgacg cgctggatca gatatttatc gccgcaccac aactttcagc agtggttctt
     1921 ccctggtccg aatggtgtga ggagccacag gcgctgacgc ttttcggaca ggtacaaagc
     1981 gacattatcc atcgttccgc tttctggcag ttaccgttat ggctgagttc tccggcaaac
     2041 cgggcctccg gtgaaatggt ttttgatgca gagcgtgaga tttatttccc gcagcgcccc
     2101 ccccgtccgc agggtgaagt ttatcgtcgt tacgatccac gcattcgcag gatgctgagt
     2161 ttccgcattg ccgatcccgt ttctgatgca gaacgtttca ctcgctggat gaacgatccg
     2221 cgcgttgagt atttctggga gcaaagtggc tcactggagg tacagatcgc ctatctggaa
     2281 cgccagctca ccagtaaaca tgcattcccg ctgattggtt gtttcgacga tcggccgttc
     2341 agttatttcg aaatctactg ggcggcggaa gaccgcattg gtcgccacta ttcgtggcag
     2401 ccctttgacc gtggcctgca tctgctggtt ggtgaacagc aatggcgcgg agcccactat
     2461 gtgcaaagct ggctgcgcgg ggtgacacat tacctgctgc tgaatgagcc acgtacgcag
     2521 cgcacagtac tggagccacg caccgataac cagcgcctgt tccgccatct tgagcctgcg
     2581 ggataccgga caattaaaga gttcgacttc ccacataaac gctcgcgcat ggtgatggcg
     2641 gatcgtcatc acttcttcac ggaggtcggt ctgtaatgaa tcacaaagac tgggatttgg
     2701 tcaaccgccg cctggtggca aaaatgttgt ctgagctgga gtatgagcag gttttccacg
     2761 cggaatctca gggcgatgac cgctactgca ttaacctgcc gggagcacaa tggcgcttca
     2821 tcgctgaacg tggtatctgg ggctggctct ggattgatgc tcaaactctg cgctgcgcgg
     2881 acgagccagt actggctcag acgctgctga tgcagctaaa gcaggtactg tcaatgagcg
     2941 atgcaaccgt tgctgagcat atgcaggatt tgtatgccac gctgctgggc gacctgcaac
     3001 tactgaaagc ccgtcgcggg ctgagcgcca gtgacctgat taatcttaat gccgaccgcc
     3061 tgcaatgcct gctgagcggt catcctaaat tcgtttttaa taaaggtcgc cgtggctggg
     3121 gtaaagaggc gctggaacga tatgcgccag agtatgccaa caccttcaga ctgcactggc
     3181 tggcggtaaa acgtgaacat atgatctggc gctgtgataa cgagatggat attcatcagt
     3241 tgttgacggc cgcaatggat ccgcaggagt ttgcccgctt cagtcaggtc tggcaggaaa
     3301 acggactgga tcataactgg ctgccgctgc cggtacatcc gtggcagtgg cagcaaaaaa
     3361 tcgctaccga cttcatcgct gattttgccg aaggcaggat ggtgtctctc ggcgagtttg
     3421 gcgaccagtg gctcgcccag cagtcgctgc gtaccctgac caacgccagt cgccggggcg
     3481 ggctggatat caagctgccg ctgaccatct acaacacctc atgctaccgg gggatccctg
     3541 gcagatacat cgctgccgga ccactggctt cacgctggct acaacaggtt tttgcgaccg
     3601 acgccaccct agtgcaaagc ggcgcagtga tacttggtga accggctgca ggctatgtct
     3661 cccatgaagg ctatgccgcg cttgcccggg ctccctatcg ctaccaggaa atgcttggtg
     3721 ttatctggcg ggagaatccg tgccgctggc tgaaaccgga tgaaagtccg gttctgatgg
     3781 caacactgat ggagtgcgac gaaaacaatc agccgctggc aggcgcatat atagaccgct
     3841 ccgggctgga cgctgaaacc tggcttacgc aactgttccg ggtggtcgtg gttcctctgt
     3901 atcacctgct ttgccgctac ggtgtcgcgc ttattgcaca tggacaaaat ataactctcg
     3961 ccatgaaaga gggggttcca cagcgtgttc tgctgaaaga cttccagggc gatatgcgac
     4021 tggtgaaaga agagttcccc gaaatggact ctttgcctca ggaggttcgt gatgttacat
     4081 cccgcctgag tgcggactac ttaatccatg atttgcagac gggtcacttc gtgacagtac
     4141 tgcgttttat ttcgccactg atggttcgtc ttggcgtacc tgaaaggcga ttttatcaac
     4201 tgctggcagc agtgttgagt gattacatga aaaaacatcc acaaatgtca gagcgttttg
     4261 cgcttttctc acttttcagg ccacaaatca ttcgcgtggt gctgaacccg gtaaaactga
     4321 cctggcctga tctggatggc ggcagccgca tgctgccgaa ttaccttgag gatctgcaaa
     4381 atccgctgtg gctggtaact caggaatatg aatcatgaaa aaaagtgtcg attttattgg
     4441 tgtagggaca gggccattta atctcagcat tgctgcgttg tcacatcaga tcgaagaact
     4501 ggactgtctc ttctttgatg aacatcctca tttttcctgg catccgggta tgctggtacc
     4561 ggattgtcat atgcagaccg tctttctgaa agatctggtc agtgctgttg cacctacaaa
     4621 tccctacagt tttgttaact atctggtgaa gcacaaaaag ttctatcgct tccttacaag
     4681 cagactacgt acagtatccc gtgaagagtt ttctgactac ctccgctggg ctgctgaaga
     4741 tatgaataac ctgtatttca gtcataccgt tgaaaacatt gatttcgata aaaaacgtcg
     4801 attgtttctg gtgcaaacca gccagggaga atattttgcc cgcaatatct gccttggtac
     4861 aggaaaacaa ccttatttac caccctgtgt gaagcatatg acacaatcct gtttccatgc
     4921 cagtgaaatg aatcttcgtc ggccggacct tagtggaaaa cggataaccg tggttggtgg
     4981 aggacagagt ggtgcagacc tgttccttaa tgcattacgc ggggaatggg gagaagcggc
     5041 ggaaataaac tgggtctccc ggcgtaataa ttttaacgca ctggatgagg ctgcttttgc
     5101 tgatgagtat tttacacctg aatatatttc aggcttctcc ggactggagg aagatattcg
     5161 ccatcagtta ctggatgagc agaaaatgac atcggatggc atcactgccg attctttact
     5221 gaccatttat cgtgagttgt accaccgttt tgaagttctg agaaaaccaa gaaatatccg
     5281 tctgctaccc agccgctcgg taacaactct ggaaagtagt ggtcctggct ggaagttgct
     5341 gatggagcat catctggatc agggcaggga gagcctggaa agtgatgtgg tgattttcgc
     5401 cacaggttac cgttctgcgt tgccacaaat acttccctca ctgatgcccc tgatcaccat
     5461 gcacgataag aacaccttta aagtgcgtga tgacttcact ctggaatgga gtggcccgaa
     5521 agagaacaac atcttcgtgg tcaacgccag tatgcaaacc catggcatcg ccgaacccca
     5581 gctcagcctg atggcatgga gatctgcacg tattcttaat cgcgtaatgg gacgtgattt
     5641 attcgatctc agtatgccgc ccgccctgat tcagtggcgc agcggcacct agggaaaacg
     5701 cagccggagg ctgcttcttt aactcgctac acagcatctt tgggctgatt ttttccgccc
     5761 gtatggagga ataatgatga taagcaaaaa gtatacgctt tgggctctca acccactgct
     5821 tcttaccatg atggcgccag cagtcgctca acaaaccgat gatgaaacgt tcgtggtgtc
     5881 tgccaaccgc agcaatcgca ccgtagcgga gatggcgcaa accacctggg ttatcgaaaa
     5941 cgccgaactg gaacagcaga ttcagggcgg caaagagctt aaagacgcac tggctcagct
     6001 gatccctggc cttgacgtca gcagccggag ccgcaccaac tacggtatga atgtgcgtgg
     6061 ccgcccgctg gtcgtgctgg ttgacggcgt gcgtctcaac tcttcacgta ccgacagccg
     6121 acaactggac tctatagatc cttttaatat cgaccatatt gaagtgatct ccggtgcgac
     6181 gtccctgtac ggcggcggca gtaccggtgg cctgatcaac atcgtgacca aaaaaggcca
     6241 gccggaaacc atgatggagt ttgaggctgg caccaaaagt ggctttagca gcagtaaaga
     6301 tcacgatgaa cgcattgccg gagctgtctc cggcggaaat gagcatatct ccggacgtct
     6361 ttccgtggca tatcagaaat ttggcggctg gtttgacggt aacggcgatg ccaccttgct
     6421 tgataacacc cagaccggcc tgcagtactc cgatcggctg gacatcatgg gaactggtac
     6481 gctgaacatc gatgaatccc ggcagcttca gttgatcaca cagtactata aaagccaggg
     6541 cgacgacgat tacgggctta atctcgggaa aggcttctct gccatcagag ggaccagcac
     6601 gccattcgtc agtaacgggc tgaattccga ccgtattccc ggcactgagc ggcatttgat
     6661 cagcctgcag tactctgaca gcgcttttct gggacaggag ctggtcggtc aggtttacta
     6721 ccgcgatgag tcgttgcgat tctacccgtt cccgacggta aatgcgaaca aacaggtgac
     6781 ggctttctct tcgtcacagc aggacaccga ccagtacggc atgaaactga ctctgaacag
     6841 caaaccgatg gacggctggc aaatcacctg ggggctggat gctgatcatg agcgctttac
     6901 ctccaaccag atgttcttcg acctggctca ggcaagcgct tccggagggc tgaacaacaa
     6961 gaagatttac accaccgggc gctatccgtc gtatgacatc accaacctgg cggtcttcct
     7021 gcaatcaggc tatgacatca ataatctctt taccctcaac ggtggcgtac gctatcagta
     7081 cactgaaaac aagattgatg atttcatcgg ctacgcgcag caacggcaga ttgccgccgg
     7141 gaaggctaca tccgccgacg ccattcctgg cggctcagtc gattacgaca acttcctgtt
     7201 caacgccggt ctgctgatgc acatcaccga acgccagcag gcatggctca acttctccca
     7261 gggcgtggag ctgccggacc cgggtaaata ctatggtcgc ggcatctatg gtgctgcagt
     7321 gaacggccat cttcctctaa caaagagtgt gaacgtcagc gacagcaagc tggaaggcgt
     7381 gaaagtcgat tcttatgagc tgggctggcg ctttactggc aataatctgc gtacccaaat
     7441 cgcggcctac tattcgattt ctgataagag cgtggtggcg aataaagatc tgaccatcag
     7501 cgtggtggac gacaaacgcc gtatttacgg cgtggaaggt gcggtggact acctgattcc
     7561 tgatactgac tggagtaccg gagtgaactt caacgtgctg aaaactgagt cgaaagtgaa
     7621 cggtacctgg cagaaatacg atgtgaagac agcaagccca tcaaaagcga cagcctacat
     7681 tggctgggca ccggacccgt ggagtctgcg cgtgcagagc accacctcct ttgacgtgag
     7741 cgacgcgcag ggctacaagg tcgatggcta taccaccgtg gatctgctcg gcagttatca
     7801 gcttccggtg ggtacactca gcttcagcat tgaaaacctc ttcgaccgtg actacaccac
     7861 tgtctggggg cagcgtgcac cactgtacta cagcccgggt tacggcccag cgtcactgta
     7921 cgactacaaa ggcaggggcc gaacctttgg tctgaactac tctgtgctgt tctgaccggt
     7981 attcctttac aacaaaggta cgctgatatc aacatggccg ctgacagcca agttgatatc
     8041 atataataca cgacataatc tgtagtcagg gaggatagac tctttactga ctacagatta
     8101 tgtcgtgttc cgtgctcatt tcctcaaaaa aatacaagaa aagaattagt attctaacaa
     8161 aaagtgaaat aaattgtatc aaactccctc ttttaatcct gttgagtaaa tcagcttttg
     8221 caataggat
//
LOCUS       DBANUU010000172         8181 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000172 DBANUU010000000
VERSION     DBANUU010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.174"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..330
                     /locus_tag="ACIE68_004130"
     CDS             <1..330
                     /locus_tag="ACIE68_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910355.1"
                     /translation="VSPIFIETEEKLRTFNTNKLTRNDNIFILFSSVDDGPVMVVSSQ
                     RLHDMLNPTKDTNWNSTYIYKSRHEMLPVNLTQETLFSSKSQDKHALFPIFTASWRAH
                     RIMNKGV"
     gene            553..1050
                     /gene="ubiC"
                     /locus_tag="ACIE68_004131"
     CDS             553..1050
                     /gene="ubiC"
                     /locus_tag="ACIE68_004131"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFX5910356.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1063..1935
                     /gene="ubiA"
                     /locus_tag="ACIE68_004132"
     CDS             1063..1935
                     /gene="ubiA"
                     /locus_tag="ACIE68_004132"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFX5910357.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2090..4513)
                     /gene="plsB"
                     /locus_tag="ACIE68_004133"
     CDS             complement(2090..4513)
                     /gene="plsB"
                     /locus_tag="ACIE68_004133"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFX5910358.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4684..5052
                     /gene="dgkA"
                     /locus_tag="ACIE68_004134"
     CDS             4684..5052
                     /gene="dgkA"
                     /locus_tag="ACIE68_004134"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFX5910359.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5162..5770
                     /gene="lexA"
                     /locus_tag="ACIE68_004135"
     CDS             5162..5770
                     /gene="lexA"
                     /locus_tag="ACIE68_004135"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFX5910360.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5843..7168
                     /gene="dinF"
                     /locus_tag="ACIE68_004136"
     CDS             5843..7168
                     /gene="dinF"
                     /locus_tag="ACIE68_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFX5910361.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQELADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7284..7493
                     /gene="yjbJ"
                     /locus_tag="ACIE68_004137"
     CDS             7284..7493
                     /gene="yjbJ"
                     /locus_tag="ACIE68_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFX5910362.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7535..8050)
                     /gene="zur"
                     /locus_tag="ACIE68_004138"
     CDS             complement(7535..8050)
                     /gene="zur"
                     /locus_tag="ACIE68_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFX5910363.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1845 a         2007 c         2241 g         2088 t
ORIGIN      
        1 gtttcaccta tatttataga aactgaagaa aagttaagaa cctttaacac taataaatta
       61 acacgaaacg ataatatatt tatcctgttc tcctctgtcg atgatgggcc tgttatggtg
      121 gtaagctccc agcgcttaca tgatatgttg aatcctacaa aagataccaa ttggaattcc
      181 acgtatatct acaaatccag acatgagatg ttgcctgtta atcttactca ggaaacactt
      241 ttcagctcca aatctcagga taaacatgcg cttttcccca tttttactgc gagttggcga
      301 gctcatcgta taatgaataa gggtgtttaa gtaaaggaaa acatcaccgt tcctggcatc
      361 ctgggcggtg atgcccctat ggttgccctc tccagcgcgg gcatcggcaa agcgtaaggt
      421 tcaacagcgt tttaccactt catgcgattg ttgcgttttt gttgcgtatt agatcactta
      481 atttgcttta catctcccgt aaacactttt ctgcgataca atgcctttac gttatgtaac
      541 ggagagttcg gcatgtcaca ccccgcgtta acgcaactgc gtgcgctgcg ctattttaaa
      601 gagatccctg ccctggaacc gcaactgctc gactggctgt tgctggagga ttccatgaca
      661 aaacgttttg aacagcaggg aaaaacggta agcgtgacga tgatccgcga agggtttgtc
      721 gagcagaatg aaatccccga agaactgccg ctgctgccga aagagtctcg ttactggtta
      781 cgtgaaattt tgttatgtgc cgatggtgaa ccgtggcttg ccggtcgtac cgtcgttcct
      841 gtgtcaacgt taagcgggcc ggagctggcg ttacaaaaat tgggtaaaac gccgttagga
      901 cgctatctgt tcacatcatc gacattaacc cgggacttta ttgagatagg ccgtgatgcc
      961 ggactgtggg ggcgacgttc ccgcctgcga ttaagcggta aaccgctgtt gctaacagaa
     1021 ctgtttttac cggcgtcacc gttgtactaa gaggaaaaaa atatggagtg gagtctgacg
     1081 cagaataagc tgctggcgtt tcatcgctta atgcgtacgg ataagccaat tggcgcgtta
     1141 ctgctgctct ggccaacatt atgggcgttg tgggtggcga caccgggcgt tcctcagctc
     1201 tggatcctgg cggtatttgt cgcgggtgtc tggctgatgc gcgctgctgg atgtgtggtg
     1261 aatgattatg ctgaccgaaa gtttgatggt catgttaagc gcacggcgaa ccgaccactt
     1321 cccagcggcg cggtaacaga gaaagaggcg cgcgcgctgt ttgtagtgct ggtactgatt
     1381 tcgtttttac tggtactgac gctgaatacg atgaccattc tgttgtcgat tgccgctctg
     1441 gcgttggcgt gggtgtaccc gtttatgaag cggtataccc atctcccgca agtggtgctg
     1501 ggcgcggcgt ttggctggtc gattccaatg gcttttgccg ctgtgagtga gtcggtgcca
     1561 ttgagttgct ggttaatgtt cctcgccaat attctctggg cggtggctta cgacacgcag
     1621 tatgcgatgg ttgaccgcga tgatgatgtg aagattggca ttaaatccac ggcaatcctg
     1681 ttcggccaat acgataaatt gattattggt attttgcaga ttggcgtact ggcactgatg
     1741 gcgatcatcg gtgagttaaa tggcttaggc tggggatatt actggtcaat tgtggtggct
     1801 ggcgcgctgt ttgtttatca acaaaaactg attgccaacc gcgagcgtga agcctgcttt
     1861 aaagcattta tgaataataa ctatgttggt ctggtactgt ttttaggact ggcaatgagt
     1921 tactggcatt tctgatgatg taaaaaagcc ggatgatcat ccggctttct tctgggttgc
     1981 cttatgcgcg gcgcttctca ggcctacaca acacatcgca atttattgaa tttgcagatt
     2041 atggaaggcc ggataaggcg ttttcgccgc atccggcaat tctctctgat tacccttcgc
     2101 cctgcgtcgc actctcaatc gtcaaacgca cgtctgatgt aatcaactcc gccagcaact
     2161 gataaacctt catcgtttct gccggttcgg cgtcgccgct atcgctgata tacccttcat
     2221 cacgcagcgt cagcaccaga gaactgaaca ccgccttgtc gaagaactcc ggcgcgttga
     2281 tgccgtgcag cacggagaga cgttgcgcga cggtgcggct ctctttctcc agcgtaccgc
     2341 ggttgatcga cgggttggca ctcaacaacc agaaggtgat ggcataacgt tgcagcgttt
     2401 cgcgcgcgcc tgcggccagc agctgtaggg tgcgagaatg cgccgggttg atatgcaact
     2461 catcatcttg cagggtaatc agcccctgac gttgcatctc atttgccagc gcatcaataa
     2521 cgtccggcaa ctcgtcgcga tcccagcgca ggaacagctc cgctttcagc attgggtaaa
     2581 gcacattgac gtgctccatc aatacgtcgc gggagatgtg gcgatgctgg gtgacaattg
     2641 ccgccatcag cgaaggcagc accaacatat gcgcaatgtt gttgcgatag taggtcatca
     2701 gcaccgcttg ctcgcgtggc agaatgatga tgtcgccgat agtgtctttc tcgacttcaa
     2761 acttgttcat ttgcagcgcg tgatcgataa gctcgctggc gctggctgaa ggaacggtag
     2821 agtccgtgga gtaaggtacg ttgcgcatca gatccaggta gcagttgagt tgctcggtta
     2881 actgctcgcg ggtgagtgag cgctgacgtg atgccagaag cgcagtacaa cacaggttca
     2941 tggcgtttgc cgcgcctgcg ttgttaatgc gtaccatcag atcggcagca atattattga
     3001 ccgtcggcgt taaccaggcc ggacgcaccg cttcgatggg atcgatagat tcacgccagt
     3061 ctggtacgtg ctggttaagg taggtcatca acggcattgg ttcaccgaag ttgacgtaac
     3121 cctgaccgag attacgcagc ttgcttaaac cgcgcagcat ctgcggcagg ctctctttct
     3181 ctttcgtcgc gccgcgcagt tctttggcgt aagtacccac ttccatgacg tgctcataac
     3241 cgatatagat cggaatcagc gtaatcggac gcgtgccgcc acgcagcatc gcctgaatgg
     3301 tcatcgacag cgtaccggtt ttcggatcca gcaaacgccc cgtgcgggaa cgaccgcctt
     3361 ccacgaagta ctcgacggaa taaccacggc tgaacagttc gccgagatac tcacggaaaa
     3421 cggtggaata aagtttattg cctttaaaag tacggcgaat aaagaacgcc cccagacggc
     3481 ggaaaatcgg cccggccggc cagaagttca ggttgatccc ggcggcgata tgcggcggca
     3541 ccagcccctg gtggtacagc acgtaagaaa gtagcaggta gtccatgtga ctgcggtggc
     3601 aaggcacata caccagctca tgaccgtcgt gggccagctg gcgaacgcgc tcggcgttat
     3661 ggacgttgat gccctgataa agtcggttcc aggtgaagcc cagaatgcgg tcagtcaggc
     3721 ggatcatctc gtaagagaaa ttcgccgcaa tctcttccat cagcgcaatc gcgttctgct
     3781 gcgctttttc atgggagatt tttttgctgc gcgcttcatc ttctaccgct ttggcaatgg
     3841 cgcgggaggc gagcagctta ttaaacagat cctgacgagc aggcagacgt gggcccacag
     3901 cagccagacg ttgacgggca aagtgcatac gcgccacgcg cgccagtttc tgtgcgatag
     3961 ttttatccgt gccgtgttca tccgccatac ggcgcagcga aactgacggc gagaaacgca
     4021 caaaactgtc gcgaccgagc cacagtacgg cgaaaaattt ctgtacgccg ttaagcatac
     4081 gcagcggcgg gtttacttcg cctttttcac gccccggcgc gcgaccaaac atcaccgata
     4141 ctggcaccat ctgcacatcc agatttgggt tgctgcggtg caaatcaaga tagtcgtgga
     4201 acagcttaat agactcttct ttcggcgtgt aataggtgaa cacacgcggc ccgccgtgaa
     4261 tgaacacata gcgcggcagt agcgtgccgt cgatttccag cggctctaac gggtcaggca
     4321 agtcatgagc cagacactgg gcgcgcaacg tcagcaaatc tgctttcgag ttgtacggta
     4381 aaacgtacat aattggacga gaggtatcca gccccagttc cggggcagga tctgccggaa
     4441 tagacttgct ttttaccagg atgcttaatg gtaaattcag taatttgtag taaattcgtg
     4501 gccagccgga cataaacgat gtaaagcctc tggttaataa tgcaaatgcg cggcaaggat
     4561 agcagaaagt catgggaaat tctgtggtat ccgctcatgt ttcgcgcggc gttacgcaaa
     4621 cccgaatcat cggatttaac ggtacactga tattgacgct cataatgtaa aaaggttctt
     4681 tcaatggcca ataataccac tggattcacc cgaattatca aagctgctgg ctattcctgg
     4741 aaaggtttac gcgctgcatg gatcaacgaa gcggcattcc gtcaggaagg cgtagcggta
     4801 ttgttggcgg tggtcatcgc ctgctggctg gatgtggacg caattacccg cgtgctgctt
     4861 atcagctccg tgatgctggt gatgattgtg gaaatcctca atagcgccat cgaagcagtg
     4921 gttgaccgaa ttggctctga ataccatgag ctttccggac gcgcaaaaga tatgggatcc
     4981 gccgcggtgc tgattgccat tatcgtcgcc gtgattacct ggtgcatcct gttatggtcg
     5041 cattttggat aacccttcca gatttcgata aatctctggt ttattgtgca gtttatggtt
     5101 ccaaaatcgc cttttgctgt atatactcac agcataactg tatatacacc cagggggcgg
     5161 aatgaaagcg ttaacggcca ggcaacaaga ggtgtttgat ctcatccgtg atcacatcag
     5221 ccagacaggt atgccgccga cgcgtgcgga aatcgcgcag cgtttggggt tccgttcccc
     5281 aaacgcggct gaagaacatc tgaaggcgct ggcacgcaaa ggcgttattg aaattgtttc
     5341 cggcgcatca cgcgggattc gtctgttgca ggaagaggaa gaagggttgc cgctggtagg
     5401 tcgtgtggct gccggtgaac cgcttctggc gcaacagcat attgaaggtc attatcaggt
     5461 cgatccttcc ttgttcaagc cgaatgctga tttcctgctg cgcgtcagcg ggatgtcgat
     5521 gaaagatatc ggcattatgg atggtgactt gctggcagtg cataaaactc aggatgtacg
     5581 taacggtcag gtcgttgtcg cacgtattga tgacgaagtt accgttaagc gcctgaaaaa
     5641 acagggcaat aaagtcgaac tgttgccaga aaatagcgag tttaaaccaa ttgtcgttga
     5701 ccttcgtcag cagagcttca ccattgaagg gctggcggtt ggggttattc gcaacggcga
     5761 ctggctgtaa catatctctg agaccgcgat gccgccaggc gtcgcggttt gtttttcatc
     5821 tctcttcatc aggcttgtct gcatggcatt cctcacttca tctgataaag cactctggca
     5881 tctcgcctta cccatgattt tctccaatat caccgttccg ttgctgggac tggtcgatac
     5941 ggcggtaatt ggtcatcttg atagcccggt ttatttgggc ggcgtggcgg ttggtgcaac
     6001 ggcgaccagc tttctcttta tgctgttgct gtttttacgc atgagcacca ccgggctgac
     6061 tgcgcaggct tatggtgcca aaaatcctca ggcattagcc cgtgcgctgg tgcaaccgtt
     6121 gctgttggcg ttgggggctg gggcgttaat tgcgctgctg cgtacaccga ttatcgatct
     6181 ggcgctgcat attgttggcg gtagtgaagc ggtcctggaa caggcgcggc gctttcttga
     6241 aatccgctgg ttaagcgctc cggcgtcgct ggcgaatctg gtattactcg gttggttact
     6301 cggcgtgcaa tatgcccgtg cgccagtaat tttgttagtg gtcggcaata tcctcaacat
     6361 tgtgctggat gtctggctgg tgatggggct gcatatgaac gtgcagggcg cggcgctggc
     6421 gacggttatt gcggaatatg caacattgct gattggtctg ctaatggtgc gtaaaatcct
     6481 caaactacgc ggaatttccg gcgaaatgct gaaaactgcc tggcgaggaa acttccgtcg
     6541 cttgctggcg cttaaccgcg atatcatgct gcgttcgctg ttgttgcaac tctgtttcgg
     6601 cgcgatcacc gtacttggtg cgcgactggg gagtgacatt atcgctgtta acgcggttct
     6661 gatgacgcta ctcaccttta ccgcctatgc gctggatggt tttgcctacg cggttgaagc
     6721 acattccggt caggcatacg gtgcgcgcga cggtagccag ttgctggatg tctggcgggc
     6781 agcgtgccgc cagtcgggga tcgtagcgct actgttttcg gtggtttatt tgctggcagg
     6841 ggaacacatc attgcgttac tgacgtcgtt aacccagatt caggagctgg ccgaccgcta
     6901 tcttatctgg caggtgattt tgccgttggt tggcgtctgg tgttatctgc tggacggcat
     6961 gtttataggc gcaacgcgtg ccgccgaaat gcgtaacagt atggcggtgg ccgccgcagg
     7021 ttttgcgctg acgctcctta cgctgccgtg gctgggtaat catggtttgt ggctggcatt
     7081 aaccgtcttt ctggcgttgc gcgggctttc tctggcggct atctggcggc gtcactggcg
     7141 caacggtacc tggtttgccg caacgtgacg gttaaaaatt ctgaataaat aatcctaagc
     7201 caaattgctg actacactta atctcacgtt cagaagaaaa gtgaacgtac tctcattcac
     7261 aacctaacga tgaggtcttg attatgaata aagatgaagc cggcggtaac tggaaacagt
     7321 ttaaaggtaa agtgaaagag caatggggca aactgaccga tgatgatatg acgatcattg
     7381 aaggtaaacg tgatcaactg gtcggtaaaa tccaggaacg ttatggttat cagaaagatc
     7441 aggcagaaaa agaggtcgtg gattgggaaa cccgcaatga atatcgctgg taattaatcc
     7501 ctcctgcccg acgtgtacaa ggatgtacgc cctcttaacg cggtttcttt ttcacctgca
     7561 cagagtgatc atgctggcac tgttcaggat gacgacacgc ttccacttcc acgcatgccg
     7621 cacagagccc atgtgcttca atcacattat gccgcagggc aaaccccatt tttgccgcca
     7681 gcgtatgcat aatgtcttcc acgccttctg cacactcttc tttcaccgcg ccgcagcgat
     7741 cgcaaataaa catggctgac gtatgcgtgg gctggtcgaa cagatgacag agcacataac
     7801 tgttggtgga ttccacctta tgcacaaaac cttgctcaag cagaaaatcc agcgcgcgat
     7861 aaaccgttgg cggcttggct tgcggttcag cttcgcgcag caaatcaagc agatcataag
     7921 cgctgatagc gccatcctgc agactcatca ggcgcaacac ttccaggcgc tgtggggtca
     7981 ggcgcacatt acgctgcgcg cagatttttt cagcctgcgc taataactcc tgcgttgtgg
     8041 tcttttccat cgggcacctc aaagtggggg ttaagaatgt ccttacttta ccatgttcca
     8101 ggaaaaacac cgagaaccct tattgttgcc gtaatgttga ttttctgttt tgtacgtaag
     8161 gtgttgtgtt gcttggtcgt a
//
LOCUS       DBANUU010000173         8077 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000173 DBANUU010000000
VERSION     DBANUU010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.176"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            94..1227
                     /gene="ompN"
                     /locus_tag="ACIE68_004139"
     CDS             94..1227
                     /gene="ompN"
                     /locus_tag="ACIE68_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFX5910364.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1368..1802
                     /gene="uspF"
                     /locus_tag="ACIE68_004140"
     CDS             1368..1802
                     /gene="uspF"
                     /locus_tag="ACIE68_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFX5910365.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1978..2913
                     /gene="ttcA"
                     /locus_tag="ACIE68_004141"
     CDS             1978..2913
                     /gene="ttcA"
                     /locus_tag="ACIE68_004141"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFX5910366.1"
                     /translation="MSQNQEISKKGQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGINHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(3042..4415)
                     /gene="dbpA"
                     /locus_tag="ACIE68_004142"
     CDS             complement(3042..4415)
                     /gene="dbpA"
                     /locus_tag="ACIE68_004142"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFX5910367.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4445..4618)
                     /gene="ynaL"
                     /locus_tag="ACIE68_004143"
     CDS             complement(4445..4618)
                     /gene="ynaL"
                     /locus_tag="ACIE68_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFX5910368.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(4893..5876)
                     /gene="zntB"
                     /locus_tag="ACIE68_004144"
     CDS             complement(4893..5876)
                     /gene="zntB"
                     /locus_tag="ACIE68_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFX5910369.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHESAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5890..5937)
                     /gene="ydaN"
                     /locus_tag="ACIE68_004145"
     CDS             complement(5890..5937)
                     /gene="ydaN"
                     /locus_tag="ACIE68_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFX5910370.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            6131..7363
                     /gene="dgcM"
                     /locus_tag="ACIE68_004146"
     CDS             6131..7363
                     /gene="dgcM"
                     /locus_tag="ACIE68_004146"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFX5910371.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7384..7947)
                     /gene="smrA"
                     /locus_tag="ACIE68_004147"
     CDS             complement(7384..7947)
                     /gene="smrA"
                     /locus_tag="ACIE68_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFX5910372.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
BASE COUNT         2136 a         2019 c         1906 g         2016 t
ORIGIN      
        1 atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga aatggggata
       61 aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gcaaagtact ggcactttta
      121 attcctgccc tgctcgccgc aggtgctgca catgcagccg aagtttataa taaagacggc
      181 aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga taattcggcg
      241 aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca aattaacgat
      301 caactcactg gctacggtca atgggaatac aatattcagg caaacaacac tgaatcttca
      361 aaaaaccagt catggacccg tctggcattt gccgggctga aatttgcaga ttacggttct
      421 ttcgattacg gacgtaatta tggcgtaatg tacgacatcg aaggctggac cgatatgctg
      481 cctgaatttg gcggtgactc ttataccaat gcagacaact ttatgactgg tcgagccaat
      541 ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg taaatggtct gaacttcgcg
      601 gtgcagtatc aaggtaacaa cgaaggtgcc agtaatggtc aggaaggcac caacaacgga
      661 cgtgatgttc gccatgaaaa cggtgacggc tggggtcttt ccacaacata tgatttaggc
      721 atgggcttta gcgctggtgc ggcatacacc tcttctgacc gcaccaatga tcaggttaac
      781 catactgcgg cgggtggtga taaagcagac gcgtggactg ctgggctaaa atacgatgct
      841 aacaatattt acctggcaac catgtattca gaaacgcgta atatgacccc gtttggcgac
      901 agcgattatg ctgttgcaaa caaaacccag aactttgaag tcactgcaca gtaccagttt
      961 gattttggtc tgcgtccggc agtctctttc ctgatgtcta aaggccgtga cctgcacgct
     1021 gccggtggtg cagacaaccc ggcaggtgtt gatgataaag atctggttaa atacgccgat
     1081 gttggcgcga cttactattt caataaaaac atgtccactt atgttgacta taaaatcaac
     1141 ctgttggatg aagatgacag tttctacgct gccaatggca tctctaccga tgatattgtc
     1201 gctttaggtc tggtttatca gttctaaatc ctcctgcccg ctgttatggc gggctttttc
     1261 tgcttattct tcctcctttg atctaaatta aaaatgtgaa ctccgtcatt acacaaaaag
     1321 tgtcatctgg cgttacactt tatgcggata ctaaaacagg aggttttatg aacagaacga
     1381 ttcttgtccc tatcgatatt tccgattcag aattaactca acgcgtgatt agccacgttg
     1441 aggcagaggc aaagattgat gatgcagagg ttcatttcct gacggtaata ccttcactgc
     1501 cctactatgc ctctctgggt ttagcgtatt ccgcagaatt accggcaatg gatgacctga
     1561 aagcggaagc caaatcgcaa ctggaagaga tcattaaaaa atttaaactg ccaaccgaca
     1621 gagtgcatgt ccatgttgag gaaggctcgc ccaaagaccg cattctggaa ttggcgaaga
     1681 agatccccgc tcatatgatc atcattgctt cccatcgacc ggatatcacc acttatctgc
     1741 tcggttccaa cgccgcagct gtagtgcgtc acgcagagtg ctccgtgctg gttgtgcgct
     1801 gacactaacg cccgcacatt gctgcgggct ttttgattca tttcgcaaat gtgctgacat
     1861 tttcccctct aatccgtacc atacacgcca cagtttttat atcagatttc ttatgctggg
     1921 cgttccggca tgacgcatac tcttctgatg ccatataacg aattgagtcg cttttaaatg
     1981 tcgcaaaatc aagaaattag taagaaagga caatacaacc tgaacaaatt acaaaaacgt
     2041 ctgcgtcgta acgtgggcga agccattgct gacttcaata tgattgaaga aggcgatcgc
     2101 atcatggttt gcctctcagg gggtaaagac agctatacca tgctggagat tctgcgcaat
     2161 ttgcagcaaa gcgcgccaat caatttttcg ctggtggctg ttaacctcga tcaaaagcaa
     2221 ccgggcttcc cagaacacgt tctgcccgag tatcttgaaa agctgggcgt tgagtacaag
     2281 attgtcgaag agaacactta cggtatcgtg aaagagaaga ttccagaggg caaaaccact
     2341 tgctcactgt gttctcgcct tcgtcgcggt atcctttatc gtaccgcaac ggaactgggg
     2401 gcgacgaaga tcgcgttggg tcaccatcgt gacgatatcc tgcaaacgtt gttcttaaat
     2461 atgttctacg gcggcaagat gaaaggtatg cctccgaaac tgatgagcga tgatggcaaa
     2521 catatcgtta ttcgtccgct ggcctactgc cgcgagaaag atattcagcg atttgccgat
     2581 gcaaaagcgt tcccgattat tccgtgcaac ctgtgcggtt cacagcctaa cctgcaacgt
     2641 caggtgattg ctgacatgtt gcgtgactgg gataaacgtt atccagggcg tatcgagacg
     2701 atgttcagcg cgatgcagaa tgtagtgccg tcgcatctgt gcgataccaa cctgttcgat
     2761 tttaaaggca ttaaccacgg ttctgaagtg gttaacgggg gtgatctggc gtttgatcgc
     2821 gaagagatcc cactacaacc ggcgggctgg cagccagaag aagatgaaaa tcagttggat
     2881 gagttacgac tgaatgtggt tgaagtgaaa taaccaggat agcgcccgat gcgcaagcgt
     2941 attgggctac tcttatggag gccggataag acgcggccag cgtcgcatcc ggcaatcccg
     3001 aataagatgt ttactcttgc acccggcaat tcaacatttc attattttaa taaccgcacc
     3061 cggcacgttt ttcctttaat cttcccgccc tgtaactgtt tccatgcttt atgagcaaca
     3121 gcctgacgga ccgcgacata gacatgcgcc ggatgcacgg cgattttgcc aatatctgcg
     3181 ccatcaagcc cgatatctcc tgtcagtgca cctaatacat cacccgggcg cattttggct
     3241 tttttcccgc catcgataca caacgttgcc atttctgctt ccagcggcac aatggaacta
     3301 ttagctggcg gcgtttgcca gttaagtttt atctgcaaca tgtcagaaat gatattggcc
     3361 cgctgtgctt cttccggagc acagaaactg atcgccagac cgctatttcc tgcacgagct
     3421 gtacgaccga tgcgatgtac atgaacttca gggtcccacg ccagctcaaa gttcaccacc
     3481 agctcaagcg atttaatatc cagaccacgc gcagcaacat cagtagcaac cagtacacgg
     3541 gcgctaccgt tagcaaaacg taccagagtc tgatcgcgat cgcgttgctc cagatcgccg
     3601 tgtaacgaca atgcactttg ccctacttca ttcagcgcgt cgcagacagc ctggcaatct
     3661 tttttggtat tgcaaaacac cacgcaagag gatggctgat gcaagcttaa taaccgttgc
     3721 aacagaggaa ttttgccttt gctggatgtc tcataaaatt gttgttcaat gggtggcaaa
     3781 gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg
     3841 gcgatggctt ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa
     3901 cggatgacat catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc
     3961 atcaccaaag tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa
     4021 cgacccggcg ttgccacgat aatatgcggc gcatgttgca acgaatcacg ctgcataccg
     4081 aacggttgac caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga
     4141 cgcaattcac ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg
     4201 gtttgaaata gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg
     4261 ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg
     4321 gcggcctgca ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg
     4381 ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc
     4441 aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt
     4501 cttcatcagg tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa
     4561 caggatcgcc aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat
     4621 ttccctccgg tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa
     4681 aaagccgact catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa
     4741 aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag
     4801 ggagatattg ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt
     4861 ttgtatacag acagccggaa aaattgcttt tgttacaacc atttactacg atgcaaccat
     4921 aaagcaacac caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc
     4981 cacccgccgc cagggatccc accaaggttg acgccaaata accctgtcag aaaggtactg
     5041 ggtaaaaaga ccattgccat caatgacatt gtataggtac gacgagctaa attttcctgc
     5101 atcacctgag cgatttcatc cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg
     5161 tcaagaccgc gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc
     5221 atccacggca aacgttcact ggcaagacga gcataaacat cacgttgcgg tgccatatag
     5281 cgacgcatca cgattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc
     5341 tgatcaagga gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattcg
     5401 ctggaatgat cggtcaacgc atcgcacaca tccaccagcc accctccgca atcggtcgga
     5461 cccgtgccct cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg
     5521 gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt
     5581 tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg tgccttcacc gagacggctg
     5641 actcggggcc gagtgctctc gcctgccagc gcatcacgta cgttattggg aagcagcggt
     5701 gttgtcgcca gccattgggc gctttcatgg tgtacataat taaggtggag ccagcaggga
     5761 tgcgcttcat caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc
     5821 atccaggcaa atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccacagtg
     5881 cctccatcat caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc
     5941 atcaccccat taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa
     6001 aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt
     6061 atctgatcat atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa
     6121 cgggatctca atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg
     6181 gattgtttcg ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat
     6241 gcaagacctc acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga
     6301 actgccgaaa tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt
     6361 tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga
     6421 aacagaaccg gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc
     6481 cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac
     6541 taactctgca ccaatgttgt tgattgaccc ctcacgagat ggccaaatcg tcgatgctaa
     6601 cctcgccgcg ctcaatttct atggttataa ccatgaaacg atgtgccaga aacatacctg
     6661 ggaaattaat atgctcgggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc
     6721 cggcggtcat aaacctttga attttgttca taaactggcg gatggatcta ctcgtcatgt
     6781 gcagacctat gccggaccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca
     6841 tgatattact gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc
     6901 gatgaccgga ttactgaatc ggcgacagtt ttatcacatt accgagccag gccaaatgca
     6961 gcatctcgcc atcgctcagg attacagctt gttgctcatc gacaccgatc gttttaaaca
     7021 cattaacgat ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgcac
     7081 ccttgaaagt tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt
     7141 cttattgcta ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgtgt
     7201 aagcgtggca aaagtgagta tttcgggctt accacgcttt accgtcagca ttggtgtggc
     7261 gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta
     7321 tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg
     7381 agatcaacga ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt
     7441 acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc ggtagcgcag tgcaatatgc
     7501 ctgaacatca tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg
     7561 cgatttatca tcccgtcctt tgccatgaat aatcagcacg ttacgcaaac catcagccat
     7621 ggcttgttga atgaaactga acatcatttt gcggcactct tccaccggct ggcgcaaaag
     7681 attcaggctc gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgttg
     7741 caacccttcc cgccgaaact ccagcggctg acttagtggg atgatgtcga gaaatccggt
     7801 ggtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt
     7861 tggatgccag tggacatcgg tagcacgttt cagcggctgg acatcttcca tggcgtcaag
     7921 aaacagcgat ttgtcgtcaa ggttcatgtt acatcctccc gcaattaaga gcgcgatatg
     7981 ataaccagac cgggtcggtc caacaacgta ttacccaaaa ttccagtaat aagttccaaa
     8041 tattgccgat attttaagca aaatgcttat gcatgat
//
LOCUS       DBANUU010000174         7822 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000174 DBANUU010000000
VERSION     DBANUU010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.177"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            304..1587
                     /gene="ydfJ"
                     /locus_tag="ACIE68_004148"
                     /pseudo
     CDS             304..1587
                     /gene="ydfJ"
                     /locus_tag="ACIE68_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            1676..3136
                     /gene="ydfI"
                     /locus_tag="ACIE68_004149"
     CDS             1676..3136
                     /gene="ydfI"
                     /locus_tag="ACIE68_004149"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFX5910373.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPTVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(3172..3375)
                     /gene="ydfZ"
                     /locus_tag="ACIE68_004150"
     CDS             complement(3172..3375)
                     /gene="ydfZ"
                     /locus_tag="ACIE68_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFX5910374.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(3553..4239)
                     /gene="rspR"
                     /locus_tag="ACIE68_004151"
                     /gene_synonym="ydfH"
     CDS             complement(3553..4239)
                     /gene="rspR"
                     /locus_tag="ACIE68_004151"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFX5910375.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(4328..5074)
                     /gene="ydfG"
                     /locus_tag="ACIE68_004152"
     CDS             complement(4328..5074)
                     /gene="ydfG"
                     /locus_tag="ACIE68_004152"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFX5910376.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5211..7256
                     /gene="dcp"
                     /locus_tag="ACIE68_004153"
     CDS             5211..7256
                     /gene="dcp"
                     /locus_tag="ACIE68_004153"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFX5910377.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAVAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(7301..7819)
                     /gene="ydeJ"
                     /locus_tag="ACIE68_004154"
     CDS             complement(7301..7819)
                     /gene="ydeJ"
                     /locus_tag="ACIE68_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFX5910378.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALVQLLQLLL"
BASE COUNT         1959 a         1813 c         1999 g         2051 t
ORIGIN      
        1 gtgatgtatc tgaatagttg aaaaaccgca gacacacctt atgcaagaac gtgctgcggt
       61 tggctggtaa atttttcgat agtgcgagta ttgaatgatt tccagccgtt attgatttta
      121 cgcgtaaatc catgaaaaaa ctacttatct gttggggagt tttttggggc atatatggga
      181 cagaaatagg ccccagatag acactgagat cgaacttagg atgcttttaa aaaaatgcaa
      241 ctatctgaaa aaacctagaa aacgccaagg aaaccacagg atgggaaaaa acacctgtga
      301 attatggatt tccagttata ttcgctcggc gcagcgttgg tgtttcatga aatatttttt
      361 cctgaatcat caacggcaat ggcgttaatt ttggcaatgg gaacctacgg tgcaggttat
      421 gtggcgcgta ttgtcggagc atttattttc ggcaaaatgg gcgacagaat agggcgtaaa
      481 aaagtgctct ttattaccat caccatgatg gggatctgta ccaccttaat tggtgtgtta
      541 ccgacctatg cacagattgg tgtttttgcc cccatcttgc tggtgacgct gcgtattatt
      601 caggggttgg gtgcaggtgc ggaaatttcc ggtgccggta cgatgctggc ggaatatgca
      661 ccaaaaggta agcgcggaat tatctcctca tttgtagcta tgggaactaa ctgcggaacc
      721 ttgagcgcaa cggcaatctg ggcctttatg ttcttcattc tcagtaaaga ggaactgctg
      781 gcgtggggat ggcgtatacc gttcctggcg agcgttgtcg tgatggtctt tgctatctgg
      841 ttacgtatga atctgaaaga aagcccggtc tttgagaagg ttaatgacag caaccaaccc
      901 acagcaaaac ctgcacctgc tggtagcatg ttccagagca aatccttctg gctggcaaca
      961 gggctgcgtt ttggtcaggc gggtaactcc gggttaattc agactttcct tgcaggctat
     1021 ttagtgcaga cgttattgtt taacaaagca attccaacag atgcattgat gattagttcg
     1081 attctcggct ttatgaccat tccgttcctt ggttggttat ccgataaaat tggtcgccgg
     1141 atcccgtata ttattatgaa tacctccgcg attgtgctgg catggccaat gctttctatc
     1201 attgtagata aaagctatgc cccgagcacc attatggttg cactgattgt gattcataac
     1261 tgtgcggtgc tgggattatt tgctctggaa aacattacca tggcagaaat gttcggctgt
     1321 aaaaaccgct ttacccggat ggctatttct aaagaaattg gtggtcttat cgcttccggt
     1381 tttggtccta tcctggcggg tattttctgc accatgacgg aatcctggta tccgatcgcc
     1441 attatgatca tggcatattc agtaattggt ttaatctctg cgctgaaaat gccagaggtg
     1501 aaagaccgtg atttaagtgc gctggaagac gctgcggaag atcaaccgcg tgttgtaaga
     1561 gctgcgcaac cttccagaag tctgtaaacc ctcaatccct tctcttaccg gagaggggat
     1621 ttttattcat ataacaaaac atatagcttg ccatatttaa atttaaggaa ttgttatggg
     1681 aaataatttg ttatcagcaa aagcgacact ccctgtttat gatcgtaata acctggcccc
     1741 aagaattatt catttaggct ttggtgcatt tcaccgtgcg catcagggcg tgtatgccga
     1801 tattcttgct acagaacatt tcagtgactg gggatattat gaggtcaact taatcggcgg
     1861 cgaacagcaa attgccgatt tacaacagca agataatctt tataccgttg cggaaatgtc
     1921 ggccgatgcg tggacggctc gcgtcgttgg cgtcgttaaa aaagccttgc acgtacagat
     1981 ggatggctta gaaaccgtgt tggctgcgat gtgtgaaccg caaatcgcga ttgtctctct
     2041 gacaatcacc gaaaaagggt atttccactc tccggcgacc ggacagttaa tgctcgatca
     2101 cccgatggtc gctgccgacg tgcaaaatcc ccaccagccg aaaacagcaa caggggtaat
     2161 tgttgaggcg ctggcccgcc gtaaagcggc aggacttccc gcatttaccg tcatgtcatg
     2221 tgacaacatg ccagaaaacg gtcatgttat gcgtgacgtt gtcacttcat acgcgaaagc
     2281 tgttgatgta aaactggcac aatggatcga agataacgtg actttcccat caacaatggt
     2341 ggaccgtatt gtgcccacag tgacagagga tacgctggcg aaaatcgaac aacttaccgg
     2401 tgtgggcgat cctgcgggcg ttgcctgtga acctttccgc cagtgggtaa tagaagataa
     2461 ctttgttgcc ggacgtccag aatgggaaaa agcgggagcc gaactggtta gtgatgtgct
     2521 gccttatgaa gagatgaagt tgcgcatgct caacggcagt cattcattcc tggcgtattt
     2581 gggttatctt gctggatatc agcacattaa tgactgcatg gaagatgaac attatcgtta
     2641 tgcggcgtat ggcttgatgt tgcaggaaca agcgccgacg ctgaaagtgc agggcgttga
     2701 tttgcaagat tacgctaacc gattaattgc acgctatagc aacccggcgt tacgtcatcg
     2761 aacctggcag attgcgatgg atggtagcca gaaattgcca cagcggatgt tggattctgt
     2821 tcgctggcat ctggcgcatg acagcaagtt cgatctgctg gcgctgggcg tcgcgggttg
     2881 gatgcgttat gtcggtggtg ttgatgaaca gggaaatccg atagaaatca gtgacccact
     2941 gttacctgtt attcagaagg ctgtacaaag tagtgccgaa gggaaagcgc gcgtccagtc
     3001 attgctggcg attaaggcaa tctttggtga tgatttgcca gacaatagct tgtttactgc
     3061 aaaagtgacg gaagcgtact tgtctttatt agcgcatggc gcgaaagcga ccgtggcgaa
     3121 atattccgtg aagtaaaaaa agcaatgccg ccatcggcgg cattcacaca cttagttcat
     3181 gccgagacga atcaggtcca gaggtgccag tttctcttca caaccttcaa caactacggt
     3241 ttttccgcgg cggatttgct cagcggtcag accttcagta tcaatcgaca ggatcttgcc
     3301 agtgtgaccg gtgccgctaa ccataacacg gctgccagtg gtgattgcgt tacggttacg
     3361 atcgtaggtc gtcataggtg ttttctcctt tctgatttac agtcgaagcc cacgtaacgg
     3421 gcgcaatata aatactcctg tcgagtgaaa tattttttgt ttttgatcaa gctcacatct
     3481 tttttgatgc atgcatgagc cgtaaggcgg ggggaaaagc ctctcatccc ggatgggatg
     3541 agagtaaatg aattactctt cgctaaacca gtcgctgttt tcctggcgga tctggcgcac
     3601 ggattcgctg atttcctgca aatgttgcgt cattgcacgt tctaccgcat cgccatcacg
     3661 tttttgcagg gcagagaaaa tatcaagatg ctggcgtaac agcatttctg gtggagaaac
     3721 atggtcgaaa ctcatatagc gcacgcgatc aacggtcgct ttcaggttct caatggtatc
     3781 ccacgccagt tgacagtcgg caatctgcgt caggagttga tggaagttgt catcaagttc
     3841 aaaaaaatca tccagttgct tgcgctcaat ggcaatgcgt tgctggtgaa gattttgttc
     3901 cagttggtag cactggcttt cggtaatcat gctcgccgcc cgacgcgcca ccgcgcactc
     3961 aatggcctga cggataaaac tgccgtttcg cacctgggcc atggaaattt tgttgacgta
     4021 gctgccacgc tgcggacgaa tttgaatcag gccgttttcc gccagtttaa taaaggcttc
     4081 acgaaccggc tggcgtgaca cattgaaacg aacagaaact tctttttccg acaacggtgt
     4141 gcctggtgca atcaggcaat ggacaatgtc gcgacgaaga atacgataaa tctgctgatt
     4201 gacaggctgt gtgggattaa gttgcgtttc gacggtcatt cgttcttgct tgtgagtgag
     4261 ttaactgcat gagtctacca cttttttgtg acaagcaata acccggcagt tacgccgggt
     4321 ataaaaatta ctgacggtgg acattcagtc cggcatagct ttgggtaacc ggcatcattt
     4381 ccagggtatt gatattgacg tgagcaggca gcgttgacac ccaccagacg gcttcgctga
     4441 catcttctgg cgtcaatgca acggtatttt gataggtttt ttccgcttta ccgtcatcgc
     4501 ctttaaagcg gacattggaa aactcggtgc cacccaccag acccggttcg atgtcggtga
     4561 cgcgcaccgc cgtaccatgc agatccgtac gcagattcag gctaaactga cgaacaaacg
     4621 ctttcgtcgc accgtaaacg ttaccaccgg catacggcca gctacctgcc gttgagccaa
     4681 tgttaataat atgaccatga ttacgttcaa ccatacccgg taagacggcg cgcgtcatat
     4741 ataccaggcc tttgttgttg gtatcaatca tcgtttccca gtcttcaacg ctggctttat
     4801 gcgcaggctc catgcccaac gccaggccgg cattatttac caggatatca atattgcacc
     4861 actcggcagg aagcgatgcc agcatctctt caatagcggc gcggttgcga acgtccagtt
     4921 gggcgatata cagattatct cccagttcgt cttttaactc ctgcaaccgc tcctggcgac
     4981 ggccagtggc gataacttta tgcccttgtt gaataaaacg acgagtaatg cattcaccaa
     5041 aacctgccgt tgctccagtt actaaaacga tcatctcact gttcctcaac gcttttgtgt
     5101 gtcaataaca tagcacgtga tgcttaagag cgggtaatac tggtttgcaa gattgcactc
     5161 cgttatcagt aagcctacgc tatggaacac ctcgcgttca ggagaagaaa atgacaacaa
     5221 tgaatccttt ccttgtgcaa agcacactgc cgtatctggc tcctcatttt gatcaaattg
     5281 ccaatcatca ctatcgcccg gcattcgatg agggaatgca gcaaaagcgg gcagaaattg
     5341 ctgccatcgc gcttaacccg caaacgcctg atttcaacaa tactattctg gcactggaac
     5401 aaagcggaga attacttacc cgcgttacca gcgtcttttt tgcgatgact gcggcgcata
     5461 ccaatgatga attacagcgt cttgatgaac agttttccgc tgaactggcg gaactggcta
     5521 atgatatcta tctgaacggt gaattattcg cgcgggtaga tgctgtctgg cagcgccgtg
     5581 aatccctggg gcttgatagt gaatccatcc gcctggtgga ggttattcat caacgttttg
     5641 tccttgccgg agccaaactt gcgcaagctg ataaagcaaa attaaaagta ctgaatacag
     5701 aagctgcgac cctgaccagc cagtttaacc agcggttact tgcagcaaat aaatccggcg
     5761 gtctggttgt gaacgatatc gcgcagctgg caggaatgag tgagcaagag attgcgctgg
     5821 cggcagtggc ggctcgcgag aaaggtctgg ataacaaatg gctgattccg ctgctgaata
     5881 ccacccaaca accggcgctt gccgaaatgc gcgatcgtgc gacgcgtgaa aaactgttta
     5941 ttgcgggctg gacgcgagcg gaaaaaaatg atggcaatga tacccgcgct atcattcaac
     6001 gtctggtaga gattcgcgca cagcaggcga aactgcttgg ttttcctcat tatgccgcat
     6061 ggaaaatcgc cgatcagatg gcaaaaacgc cagaagcagc actcaacttt atgcgggaaa
     6121 ttgttccagc ggcgcgtcaa cgtgcgagcg atgaattagc ctccatacag gcggttatcg
     6181 ataagcagca gggcgggttt agcgcgcagc cgtgggactg ggcattttat gcggaacagg
     6241 tacggcggga gaaatttgat cttgatgagg cgcagctcaa gccatatttt gaattaaaca
     6301 cggtgttgaa tgaaggtgta ttctggaccg cgaatcagct cttcggtatt aagtttgtcg
     6361 aacgttttga tattcctgtc taccatcctg acgttcgggt gtgggaaatt tttgatcata
     6421 atggcgtggg gctggcgtta ttttacggtg atttcttcgc ccgtgattca aaaagcggcg
     6481 gtgcatggat gggcaatttt gttgagcaat caacgcttaa tgaaacgcat ccggtaattt
     6541 ataacgtttg caattatcag aaacccgctg ccggtgagcc tgcgttgtta ctctgggatg
     6601 atgtcataac cttattccat gaatttggtc atacgttgca cggccttttt gcccgccagc
     6661 gttatgccac gctttccggc accaacacgc cgcgtgattt tgtcgaattt ccgtcgcaaa
     6721 tcaacgaaca ctgggcaacg catccgcagg tattcgctcg ctacgcccgg cattatcaga
     6781 gcggggcagc aatgcctgac gaactgcaac agaaaatgcg taatgccagc ctgttcaaca
     6841 aagggtatga gatgagcgaa ctgcttagcg ccgcacttct cgatatgcgc tggcattgcc
     6901 tggaagaaaa cgaagcaatg caggatgtcg atgattttga attgcgggcg ctggtggcgg
     6961 aaaatatgga tcttcctgct ataccgccac gctatcgcag cagttatttc gcccatattt
     7021 ttggtggcgg atatgctgca ggttattacg cttatctgtg gacgcaaatg ttggccgatg
     7081 atggttacca gtggtttgtt gagcagggcg gattaacgcg tgaaaatggg cagcgttttc
     7141 gcgaggcgat cctttccaga ggtaacagcg aagatctgga acggctgtat cgacaatggc
     7201 gcggtaaggc tcctcagatt atgccgatgc tgcaacatcg tggcttgaac atataaacac
     7261 caccaatggc tggcctggag atatcgccag gccagcctgg ttatagcagt aattgcagca
     7321 gctggacgag ggcaaacctc accgctaaag ctaataccgt ttcgcagtcg ccagcaaaat
     7381 gcataaccgc agtgtaggtc tggcctttaa tatgccacgc gaaccagacg gtacccgctg
     7441 gcgtaccatc ttcaccgccc tccggtccgc cgtagccggt aatggcaata ctgacatcag
     7501 catccgcacg ctctatggca cctgttgcca tttctgctgc cactttctca ctcaccgcag
     7561 aatatcgttc aagagattgc tggcttacgc tgaggatttt catctttgcc tgatcggtga
     7621 aagtaacaaa gcctgcaccg taaaatttgg gtgtatcttc agctgcacac agggcgctag
     7681 ccaacttacc gccggtgcat gattcggcgg tagttaagcg taattgatcc gcgataagtc
     7741 tttgactcaa cgcggatgtc aggttttcaa tagtgtcggt atctgcgagt tgtacaattt
     7801 tatctttatt aatgttcact at
//
LOCUS       DBANUU010000175         7783 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000175 DBANUU010000000
VERSION     DBANUU010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.178"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            283..1047
                     /gene="pdhR"
                     /locus_tag="ACIE68_004155"
     CDS             283..1047
                     /gene="pdhR"
                     /locus_tag="ACIE68_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFX5910379.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            1208..3871
                     /gene="aceE"
                     /locus_tag="ACIE68_004156"
     CDS             1208..3871
                     /gene="aceE"
                     /locus_tag="ACIE68_004156"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFX5910380.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            3886..5778
                     /gene="aceF"
                     /locus_tag="ACIE68_004157"
     CDS             3886..5778
                     /gene="aceF"
                     /locus_tag="ACIE68_004157"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFX5910381.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            5986..7410
                     /gene="lpdA"
                     /locus_tag="ACIE68_004158"
     CDS             5986..7410
                     /gene="lpdA"
                     /locus_tag="ACIE68_004158"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFX5910382.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(7481..>7783)
                     /locus_tag="ACIE68_004159"
     CDS             complement(7481..>7783)
                     /locus_tag="ACIE68_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFX5910383.1"
                     /translation="PQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQ
                     RVQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSAVRERLSERGARRQER"
BASE COUNT         1978 a         1949 c         2128 g         1728 t
ORIGIN      
        1 tttaattgaa ttaaaaatca caaaattggt aagtgaatcg gttcaattcg gatttttata
       61 gtttaataat cgttaaaaaa ctcctttcct acgtaaagtc tacatttgtg catagttaca
      121 actttgaaac gttatatatg tcaagttgtt aaaatgtgca cagtttcatg atttcaatca
      181 aaacctgtat ggacataagg tgaatacttt gttactttag cgtcacagac atgaaattgg
      241 taagaccaat tgacttcggc aagtggctta agacaggaac tcatggccta cagcaaaatc
      301 cgccaaccaa aactctccga tgtgattgag cagcaactgg agtttttgat cctcgaaggc
      361 actctccgcc cgggcgaaaa actcccaccg gaacgcgaac tggcaaaaca gtttgacgtc
      421 tcccgtccct ccttgcgtga ggcgattcaa cgtctcgaag cgaagggctt gttgcttcgt
      481 cgccagggtg gcggcacttt tgtccagagc agcctatggc aaagcttcag cgatccgctg
      541 gtggagctgc tctccgacca tcctgagtca cagtatgact tgctcgaaac acgacacgcc
      601 ctggaaggta tcgccgctta ttacgccgcg ctgcgtagta ccgatgaaga caaggaacgc
      661 atccgtgaac tccaccacgc catagagctg gcgcagcagt ctggcgatct ggacgcggaa
      721 tcaaacgccg tactccagta tcagattgcc gtcaccgaag cggcccacaa tgtggttctg
      781 cttcatctgc taaggtgtat ggagccgatg ttggcccaga atgtccgcca gaacttcgaa
      841 ttgctctatt cgcgtcgcga gatgctgccg ctggtgagta gtcaccgcac ccgcatattt
      901 gaagcgatta tggccggtaa gccggaagaa gcgcgcgaag catcgcatcg ccatctggcc
      961 tttatcgaag aaattttgct cgacagaagt cgtgaagaga gccgccgtga gcgttctctg
     1021 cgtcgtctgg agcaacgaaa gaattagtga tttttctggt aaaaattatc cagaagatgt
     1081 tgtaaatcaa gcgcatataa aagcgcggca actaaacgta gaacctgtct tattgagctt
     1141 tccggcgaga gttcaatggg acaggttcca gaaaactcaa cgttattaga tagataagga
     1201 ataacccatg tcagaacgtt tcccaaatga cgtggatccg atcgaaactc gcgactggct
     1261 ccaggcgatc gaatcggtca tccgtgaaga aggtgttgag cgtgctcagt atctgatcga
     1321 ccaactgctt gctgaagccc gcaaaggcgg tgtaaacgta gccgcaggca caggtatcag
     1381 caactacatc aacaccatcc ccgttgaaga acaaccggag tatccgggta atctggaact
     1441 ggaacgccgt attcgttcag ctatccgctg gaacgccatc atgacggtgc tgcgtgcgtc
     1501 gaaaaaagac ctcgaactgg gcggccatat ggcgtccttc cagtcttccg caaccattta
     1561 tgatgtgtgc tttaaccact tcttccgtgc acgcaacgag caggatggcg gcgacctggt
     1621 ttacttccag ggccacatct ccccgggcgt gtacgctcgt gctttcctgg aaggtcgtct
     1681 gactcaggag cagctggata acttccgtca ggaagttcac ggcaatggcc tctcttccta
     1741 tccgcacccg aaactgatgc cggaattctg gcagttcccg accgtatcta tgggtctggg
     1801 gccgattggt gctatttacc aggctaaatt cctgaaatat ctggaacacc gtggcctgaa
     1861 agatacctct aaacagaccg tttacgcgtt cctcggcgac ggtgaaatgg acgaaccgga
     1921 atccaaaggt gcgatcacca tcgctacccg tgaaaaactg gataacctgg tcttcgttat
     1981 caactgtaac ctgcagcgtc ttgacggccc ggtcaccggt aacggcaaga tcatcaacga
     2041 actggaaggc atcttcgaag gtgctggctg gaacgtgatc aaagtgatgt ggggtagccg
     2101 ttgggatgaa ctgctgcgta aagataccag cggtaaactg atccagctga tgaacgaaac
     2161 cgttgacggc gactaccaaa ccttcaaatc gaaagatggt gcgtacgttc gtgaacactt
     2221 cttcggtaaa tatcctgaaa ccgcagcact ggttgcagac tggactgacg agcagatctg
     2281 ggcactgaac cgtggtggtc acgatccgaa gaaaatctac gctgcattca agaaagcgca
     2341 ggaaaccaaa ggcaaagcga cagtaatcct tgctcatacc attaaaggtt acggcatggg
     2401 cgacgcggct gaaggtaaaa acatcgcgca ccaggttaag aaaatgaaca tggacggcgt
     2461 gcgtcacatc cgcgaccgtt tcaatgtgcc ggtgtctgat gcagatatcg aaaaactgcc
     2521 gtacatcacc ttcccggaag gttctgaaga gcatacctat ctgcacgcac agcgtcagaa
     2581 actgcacggt tatctgccaa gccgtcagcc gaacttcacc gagaagcttg agctgccgag
     2641 cctgcaagac ttcggcgcgc tgctggaaga gcagagcaaa gagatctcta ccactatcgc
     2701 tttcgttcgt gccctgaacg taatgctgaa gaacaagtcg atcaaagatc gtctggtacc
     2761 gatcatcgcc gacgaagcgc gtactttcgg tatggaaggt ctgttccgtc agattggtat
     2821 ttacagcccg aacggtcagc agtacacccc gcaggaccgc gagcaggttg cttactataa
     2881 agaagacgag aaaggtcaga ttctgcagga agggatcaac gagctgggcg caggttgttc
     2941 ctggctggca gcggcgacct cttacagcac caacaatctg ccgatgatcc cgttctacat
     3001 ctattactcg atgttcggct tccagcgtat cggcgatctg tgctgggcgg ctggcgacca
     3061 gcaagcgcgt ggcttcctga tcggcggtac ttccggtcgt accaccctga acggtgaagg
     3121 tctgcagcac gaagatggtc acagccacat tcagtcgctg actatcccga actgtatctc
     3181 ttacgacccg gcttacgctt acgaagttgc tgtcatcatg catgacggtc tggagcgtat
     3241 gtacggtgaa aaacaagaga acgtttacta ctacatcact acgctgaacg aaaactacca
     3301 catgccggca atgccggaag gtgctgagga aggtatccgt aaaggtatct acaaactcga
     3361 aaccattgaa ggtagcaaag gtaaagttca gctgctgggc tccggttcta tcctgcgtca
     3421 cgtccgtgaa gcggcagaga tcctggcgaa agattacggc gtaggttctg acgtttatag
     3481 cgtgacctcc ttcaccgagc tggcgcgtga tggtcaggat tgtgaacgct ggaacatgct
     3541 tcatccgctg gaaactccgc gcgttccgta tatcgctcag gtgatgaacg acgctccggc
     3601 agtggcatct accgactata tgaaactgtt cgctgagcag gtccgtactt acgtaccggc
     3661 tgacgactac cgcgtactgg gtactgatgg cttcggtcgt tccgacagcc gtgagaacct
     3721 gcgtcaccac ttcgaagttg atgcttctta tgtcgtggtt gcggcgctgg gcgaactggc
     3781 taaacgtggc gaaatcgata agaaagtggt tgctgacgca atcgccaaat tcaacatcga
     3841 tgcagataaa gttaacccgc gtctggcgta agaggtaaaa gaataatggc tatcgaaatc
     3901 aaagtaccgg acatcggggc tgatgaagtt gaaatcaccg agatcctggt caaagtgggc
     3961 gacaaagttg aagccgaaca gtcgctgatc accgtagaag gcgacaaagc ctctatggaa
     4021 gttccgtctc cgcaggcggg tatcgttaaa gagatcaaag tctctgttgg cgataaaacc
     4081 cagaccggcg cactgattat gattttcgat tccgccgacg gtgcagcaga cgctgcacct
     4141 gctcaggcag aagagaagaa agaagcagct ccggcagcag cacccgcggc tgcggcggca
     4201 aaagacgtta acgttccgga tatcggcagc gacgaagttg aagtgaccga aatcctggtg
     4261 aaagttggcg ataaagttga agctgaacag tcgctgatca ccgtagaagg cgataaagct
     4321 tctatggaag ttccggctcc gtttgctggc accgtgaaag agatcaaagt gaacgtgggt
     4381 gacaaagtgt ctaccggctc gctgattatg gtcttcgaag tcgcgggtga agcaggcgcg
     4441 gcagctccgg cggctaaaca ggaagcggct ccggcagcgg cccctgcacc agcggctggc
     4501 gtgaaagaag ttaacgttcc ggatatcggc ggtgacgaag ttgaagtgac cgaagtgatg
     4561 gtgaaagtgg gcgacaaagt tgccgctgaa cagtcactga ttaccgtaga aggcgacaaa
     4621 gcttctatgg aagttccggc tccgtttgca ggcgtcgtga aggaactgaa agtcaacgtt
     4681 ggcgataaag tgaaaactgg ctcgctgatt atgatcttcg aagttgaagg cgcagcgcct
     4741 gcggcggctc cggcgaaaca ggaagcggca gcgccggctc cggcagcaaa agctgaagct
     4801 ccggcagcag ctccggctgc gaaagccgaa ggcaaatctg aatttgctga aaacgacgct
     4861 tacgttcacg cgactccgct gatccgccgt ctggcacgcg agtttggcgt taacctggcg
     4921 aaagtgaagg gcactggccg taaaggtcgt atcctgcgcg aagacgttca ggcttacgtg
     4981 aaagaagcta tcaaacgtgc agaagcagct ccggcggcga ctggcggcgg tatcccaggc
     5041 atgctgccgt ggccgaaggt ggacttcagc aagtttggtg aaatcgaaga agtggaactg
     5101 ggccgtatcc agaaaatttc tggtgcgaac ctgagccgta actgggtgat gatcccgcat
     5161 gttactcact tcgacaaaac cgatatcacc gagctggaag cgttccgtaa acagcagaac
     5221 gaagaagcgg cgaaacgtaa gctggatgtg aagatcaccc cggttgtctt catcatgaaa
     5281 gccgttgctg cagctcttga gcagatgcct cgcttcaata gttcgctgtc ggaagacggt
     5341 cagcgtctga ccctgaagaa atacatcaac atcggtgtgg cggtggatac cccgaacggt
     5401 ctggttgttc cggtattcaa agacgtcaac aagaaaggca tcatcgagct gtctcgcgag
     5461 ctgatgacta tttctaagaa agcgcgtgac ggtaagctga ctgcgggcga aatgcagggc
     5521 ggttgcttca ccatctccag catcggcggc ctgggtacta cccacttcgc gccgattgtg
     5581 aacgcgccgg aagtggctat cctcggcgtt tccaagtccg cgatggagcc ggtgtggaat
     5641 ggtaaagagt tcgtgccgcg tctgatgctg ccgatttctc tctccttcga ccaccgcgtg
     5701 atcgacggtg ctgatggtgc ccgtttcatt accatcatta acaacacgct gtctgacatt
     5761 cgccgtctgg tgatgtaagt aaaagagccg gcccaacggc cggctttttt ctggtaatct
     5821 catgaatgta ttgaggttat tagcgaatag acaaatcggt tgccgtttgt tgtttaaaaa
     5881 ttgttaacaa ttttgtaaaa taccgacgga tagaacgacc cggtggataa tgggcgtcat
     5941 aaaaaaaacg tcagacccgc cggagataaa tatatagagg tcatgatgag tactgaaatc
     6001 aaaactcagg tcgtggtact tggggcaggc cccgcaggtt actccgctgc cttccgttgc
     6061 gctgatttag gtctggaaac cgtaatcgta gaacgttaca acacccttgg cggtgtttgc
     6121 ctgaacgtcg gctgtatccc ttctaaagca ctgctgcacg tagcaaaagt tatcgaagaa
     6181 gccaaagcgc tggctgaaca cggtatcgtc ttcggcgaac cgaaaaccga tattgacaag
     6241 attcgtacct ggaaagagaa agtgatcaat cagctgaccg gtggtctggc tggtatggcg
     6301 aaaggccgca aagtcaaagt ggtcaacggt ctgggtaaat ttaccggggc taacaccctg
     6361 gaagttgaag gtgagaacgg taaaaccgtg atcaacttcg acaacgcgat cattgcagcg
     6421 ggttctcgcc cgatccaact gccgtttatt ccgcatgaag atccgcgtat ctgggactcc
     6481 actgacgcgc tggaactgaa agaagtacca gaacgcctgc tggtaatggg tggcggtatc
     6541 atcggtctgg aaatgggcac cgtataccac gcgctgggtt cacagattga cgtggttgaa
     6601 atgttcgacc aggttatccc ggcagctgac aaagacatcg ttaaagtctt caccaagcgt
     6661 atcagcaaga aattcaacct gatgctggaa accaaagtta ccgccgttga agcgaaagaa
     6721 gacggtattt atgtgacgat ggaaggcaaa aaagcacccg ctgaaccgca gcgttacgac
     6781 gccgtgctgg tagcgattgg tcgtgtgccg aacggtaaaa acctcgacgc aggcaaagct
     6841 ggcgtggaag tggacgaccg tggtttcatc cgcgttgaca aacagctgcg taccaacgta
     6901 ccgcacatct ttgctatcgg cgatatcgtc ggtcagccga tgctggcaca caaaggtgtt
     6961 cacgaaggtc acgttgccgc tgaagttatc gccggtaaga aacactactt cgatccgaaa
     7021 gttatcccgt ccatcgccta taccgaacca gaagttgcat gggtaggtct gactgagaaa
     7081 gaagcgaaag agaaaggcat cagctatgaa accgccacct tcccgtgggc tgcttctggt
     7141 cgtgctatcg cttccgactg cgcagacggt atgaccaagc tgattttcga caaagaatct
     7201 caccgtgtga tcggtggtgc gattgtcggt accaacggcg gcgagctgct gggtgaaatc
     7261 ggcctggcaa tcgaaatggg ttgtgatgct gaagacatcg cactgaccat ccacgcgcac
     7321 ccgactctgc acgagtctgt gggcctggcg gcagaagtgt tcgaaggtag cattaccgac
     7381 ctgccgaacc cgaaagcgaa gaagaagtaa ttgttcgttt gccggaacat ccggcaatta
     7441 aaaaagcggc taaccacgcc gcttttttta cgtctgcaat ttacctttcc tgtcgccttg
     7501 ctccgcgctc agagagacgt tcgcgtactg ctgaccgttg ctcgttattc agcctgacag
     7561 tatggttact gtcgtttaga cgttgtgggc ggctctcctg aactttttcc cgaaaaacct
     7621 gacgttgttc aggtgatgct gattgaacac gctggcgggc gttatcacgt tgctgttggt
     7681 tcagtgggcg ctgctgtact ttttccttaa acacctggcg ctgctcaggc gaggctgact
     7741 gaatacgctc acgcgctgcg tctcttcgct gctggatctg tgg
//
LOCUS       DBANUU010000176         7735 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000176 DBANUU010000000
VERSION     DBANUU010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.179"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(167..430)
                     /gene="cedA"
                     /locus_tag="ACIE68_004160"
     CDS             complement(167..430)
                     /gene="cedA"
                     /locus_tag="ACIE68_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFX5910384.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            613..2874
                     /gene="katE"
                     /locus_tag="ACIE68_004161"
     CDS             613..2874
                     /gene="katE"
                     /locus_tag="ACIE68_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFX5910385.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKIADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2921..3679)
                     /gene="chbG"
                     /locus_tag="ACIE68_004162"
     CDS             complement(2921..3679)
                     /gene="chbG"
                     /locus_tag="ACIE68_004162"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFX5910386.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3692..5044)
                     /gene="celF"
                     /locus_tag="ACIE68_004163"
                     /gene_synonym="chbF"
     CDS             complement(3692..5044)
                     /gene="celF"
                     /locus_tag="ACIE68_004163"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFX5910387.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5149..5991)
                     /gene="chbR"
                     /locus_tag="ACIE68_004164"
     CDS             complement(5149..5991)
                     /gene="chbR"
                     /locus_tag="ACIE68_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFX5910388.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(5999..6349)
                     /gene="chbA"
                     /locus_tag="ACIE68_004165"
     CDS             complement(5999..6349)
                     /gene="chbA"
                     /locus_tag="ACIE68_004165"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFX5910389.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKIKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6400..>7735)
                     /gene="chbC"
                     /locus_tag="ACIE68_004166"
     CDS             complement(6400..>7735)
                     /gene="chbC"
                     /locus_tag="ACIE68_004166"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFX5910390.1"
                     /translation="EKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAMFVLINNVF
                     LSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFIGMALAEER
                     KVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVAEMFTFIVR
                     RNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQIIMDTISTPL
                     ASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQYGSVEAAL
                     AAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGIFQIN
                     EPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGLGAFF
                     NTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANALKF"
BASE COUNT         2082 a         2019 c         1726 g         1908 t
ORIGIN      
        1 ttaaccattt taaaataaat aaacaattaa agaaaaaaga tcacttattt atagcaatag
       61 ctcgtcaaag gcagcttttt gttacaggtg gtttgaatga atgtagcaac gaaatgcaga
      121 atttcaggtc atgtaactcc cggcaaaacc gggaggtatg taatccttac tcagtcactt
      181 ccccttcctg gcggatctga tttgcccaac gttgggcaga ttcaggcaca gtaaacgccg
      241 gtgagcgcag aaatgactct cccatcagta caaacgcaac atatttgcca cgcagcatcc
      301 agacatcacg aaacgaatcc atctttatcg catgttctgg cggcgcgggt tccgtgcgtg
      361 ggacatagct aataatctgg cggttttgct ggcggagcgg tttcttcatt actggcttca
      421 ctaaacgcat attaaaaatt agaaaaactg tagtttagcc gatttagccc ctgtacgtcc
      481 cgctttgcgt gtatttcata acaccgtttc cagaatagtc tccgaagcgg gatctggctg
      541 gtggtctata gttagagagt tttttgacca aaacagcggc cctttcagta ataaattaag
      601 gagacgagtt caatgtcgca acataacgaa aagaacccac atcagcacca gtcaccacta
      661 cacgattcca gcgaagcgaa accggggatg gactcactgg cacctgagga cggatctcat
      721 cgtccagcgg ctgaaccaac accgccaggt gcacaaccta ccgccccagg gagcctgaaa
      781 gccccagata cgcgtaacga aaaacttaat tctctggaag acgtacgcaa aggcagtgaa
      841 aattatgcgc tgaccacaaa tcagggcgtg cgcatcgccg acgatcaaaa ctcactgcgt
      901 gccggtagcc gtggtccaac gctgctggaa gattttattc tgcgcgagaa aatcacccac
      961 tttgaccatg agcgcatccc ggaacgtatt gttcatgcac gcggatcagc agctcacggt
     1021 tatttccagc catataaaag cttaagcgat atcaccaaag cggatttcct ctcagatccg
     1081 aacaaaatca ccccagtatt tgtacgtttc tctaccgttc agggtggcgc tggctctgcc
     1141 gataccgtgc gtgatatccg tggctttgcc accaagtttt atactgaaga gggtattttt
     1201 gaccttgttg gcaataacac gccaatcttc tttatccagg atgcgcataa attccccgat
     1261 tttgttcatg cggtaaaacc agaaccgcac tgggcaattc cacaagggca aagtgcccac
     1321 gatactttct gggattatgt ttctctgcaa cctgaaactc tgcacaacgt gatgtgggcg
     1381 atgtcggatc gcggcatccc ccgcagttac cgcaccatgg aaggcttcgg tattcacacc
     1441 ttccgcctga ttaatgccga agggaaggca acgtttgtac gtttccactg gaaaccactg
     1501 gcaggtaaag cctcactcgt ttgggatgaa gcacaaaaac tcaccggacg tgacccggac
     1561 ttccaccgcc gcgagttgtg ggaagccatt gaagcaggcg attttccgga atacgaactg
     1621 ggcttccagt tgattcctga agaagatgaa ttcaagttcg acttcgatct tctcgatcca
     1681 accaaactga tcccggaaga actggtgccc gttcagcgtg tcggcaaaat ggtgctcaat
     1741 cgcaatccgg ataacttctt tgccgaaaac gaacaagcag cattccatcc aggtcatatt
     1801 gttcctggtc tggacttcac caacgatccg ctgttgcagg gacgtttgtt ctcctatacc
     1861 gatacacaaa tcagtcgtct tggtgggccg aatttccatg agattccgat taaccgtccg
     1921 acctgccctt accacaactt ccagcgtgat ggcatgcatc gtatggggat cgacactaac
     1981 ccggcgaatt acgaaccgaa ctcgatcaac gataactggc cgcgcgaaac accgccggga
     2041 ccgaaacgcg gcggttttga atcatatcag gaacgcgtgg aaggaaataa agttcgtgag
     2101 cgcagtccat cgtttggcga atattattcc catccgcgtc tgttctggct aagtcagacg
     2161 ccattcgagc agcgccatat tgtcgatggt ttcagttttg agttaagcaa agtcgttcgt
     2221 ccgtatattc gtgagcgcgt tgttgaccag ctggcgcata ttgatctcac tctggcccag
     2281 gcggtggcga aaaatctcgg tatcgaactg actgacgacc agctgaatat caccccacct
     2341 ccggacgtca acggtctgaa aaaggatcca tccttaagtt tgtacgccat tcctgacggt
     2401 gatgtgaaag gtcgcgtggt agcgatttta cttaatgatg aagtgagatc ggcagacctt
     2461 ctggccattc tcaaggccct gaaggccaaa ggcgttcatg ccaaactgct ctactcccga
     2521 atgggtgaag tgactgcgga tgacggtacg gtgttgccta tagccgctac ctttgccggt
     2581 gcgccttcgc tgacggtcga tgcggtcatt gtcccttgcg gcaatatcgc ggatatcgct
     2641 gacaacggcg atgccaacta ctacctgatg gaagcctaca aacaccttaa accgattgcg
     2701 ctggcgggag acgcgcgcaa gtttaaagca acaatcaaga tcgctgacca gggtgaagaa
     2761 gggattgtgg aagctgacag cgccgacggt agttttatgg atgaactgtt aacgctgatg
     2821 gcagcacacc gcgtgtggtc acgcattcct aagattgaca aaattccggc gtaaatatgg
     2881 ataaggtcac gtcccctggg gagtgacctt accaccttac ttacacatca agataactac
     2941 ccaaccgata accacgctca gcaattgcat atttcaacga cgctgatgtc agcacttcca
     3001 gctccgttaa ccgtgggaaa cagtaagcac tctggcgaat cgtattatcg acaaacgccg
     3061 gatggcacat gacttccagc gagcgttccc cgcgatggct ggaatcatca agcacttgca
     3121 ggaacagtgc ctcgctaatc tcttcaccat aaaacgcgct actgaatccg tgcgaactgc
     3181 gcagattagc cggtaaatca ccagcgttag atagcggctg acggtcgatg cgtaacgcaa
     3241 tcccctcttc agccgcaaac ctggcaacaa tagggaaaat ctgcgggaac atatgcacat
     3301 gatgatggct atcaagatgc gtaggtttgc gtccaaatag ctcaatgaaa cgcaaatact
     3361 gactggcaag ctcctgagta atttcttcca gcggtaaagc atcttcttct gccaactgcc
     3421 agatccattt tcccagcaca ccatcgcggg ttaaccccgg catagctgtc agtggcttgc
     3481 ccatagtaag gacaaagtgc atccctatgg ccagactcgg ttcatcacga ctcaactgca
     3541 ccgcatggtc aatagcctgc ccattcacca gcgccgtcgt cgatgtgaca atcccattgc
     3601 gacaggcctc gataatgccg tagttctgac ctttgcttaa gccaaaatca tcggcattaa
     3661 caatcagtaa gcgttccata atcagcctcg gttaatgtgc ttttttaagc tctgcgatgc
     3721 agtcggcaaa atttggcagc catttctcgt gcgccagaat catctcgcgt gccagcagct
     3781 cagcatcgcg atcggaatgc accaacggac taaggtttag cgccagtaac acatcgttaa
     3841 attctccgct aagtgcggcg ttgctggcag caatctcgaa gcctttaatg gtgtgaatca
     3901 gccccatcac tttatcatcg aaatgcgtaa tgcgcggatg tggcgtcgcg ccatcgcgcc
     3961 ccagcttaca ggtcatttct accgcccagt ctgccggaat attatcaata tgcccatgat
     4021 gcgggatatt aacgtaatgt tcagcttgct tgtcgttgta gatagcgttg atcacttcgc
     4081 acgctgcatc agagtaataa gccccaccgc gctgttccag ttctttcggc ttaactttca
     4141 actccggatt tttatacagc tcaaaaagtt gtttctcgac tttctgtact acctgtgctc
     4201 gtgcgccgcc tttgtagtat tcgcccattt caatagccag catctctttc tgcttgaagt
     4261 aatacagcag ataagaacat ggcagcagat ttaacgaacg aattaagccc tcactaaatg
     4321 gcagatcgaa aatatttttt acagaggacg cttttaactg ccctgacgcc acaccatcaa
     4381 gcaattcggc aaagcgcgac ttgccattta ccagcacatc cttaatgaac accatatggt
     4441 tgaggccgaa cagatcgata gataaatcat cgctgtcttt cagcatcaga acatcgcgaa
     4501 taaacatctt catgccgatc ggaatattac acacgccgat aaagcgttta aatccggtat
     4561 gacgataaac ggcttcagtg accattcccg ccgggttagt gaagttaatc acccatgcat
     4621 tcggacaaag ttcttcgaca tcttttacga tgtcaaaaat caccggaatg gtacgcagac
     4681 ctttaaacag accgcccgcg ccgttggttt cctgaccaag ataaccatga cttaatggaa
     4741 tacgttcatc cagttcgcgc gccggtaatt ggcctacgcg cagttgggta gtaacgaaat
     4801 cagcatcttt caatgcttcg cggcgatcca gcgttttata aagcttcatc gggacgccag
     4861 cgttatcaat catccgttgg cagagttcaa aaatgatatc cagtttctct ttaccatctt
     4921 cgacatccac cagccataat tcgctgaccg gcaattcgtg ataacgctta ataaatcctt
     4981 ccagtaactc cggggtatag ctgctcccgc caccaatagt gacgactttt aatttctggc
     5041 tcataatttc tcccttcagt acagaatact gatatctggc atatctgccc ccccggacat
     5101 aaataaccca gcaacaggac agatatgtga attgtcaggt ataacgactt actgattaaa
     5161 ttcagtcaat ttcttacgat aactcttagg cgtaaaggac gttaatttct taaacgtttt
     5221 aataaacaaa ctcgggctac tataaccggc ctcaaacgca atatccgtca cggaatagtt
     5281 ggtcatttcc agttgttttt tggcaaaatt aatacggatc tcattaataa tctgcattgg
     5341 tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat tcctgtgatt tggctgacaa
     5401 cgtcaccata ttctccagcg ccgattcact aaactgctct ttatcatgca tcttttctac
     5461 cgtacttttc agccactgcg gtacatcatc aatcacctgt tcttcgcggt aatgacgtaa
     5521 acggttaatg acataaaaag taaccatctc aacaaactct tccagccctg tttcgcggaa
     5581 attcaatgaa gaaatcactg tttccacata ggtgagaaaa gcgttattgg tccggtatac
     5641 ctgcgaagcg acaaagcaat aaggcaacaa cggcaggtaa tgctgctcaa aaaagcgttt
     5701 actgatccca acgttcaata tacgcgtggc accaaactca taaaaacttt gatggtgcga
     5761 acctaacgga ataaaaacaa aatcgccccg ttccagtaac acgcgcttac cgttaatctc
     5821 ctggaaataa cgcccggtta ataccagagt aaattcataa tagtcgtgct gatgcagccc
     5881 gctgatactc tcagttttgt tatagataaa cacatggaag tttttgccat taaacaactg
     5941 ctgttctcgg gcagtggcaa tttccggcgc gttaatcact ggctgcatca tcgactcctt
     6001 atgccttcag tttttcatga agctcaatta attcagtaat cagttcacgc gcaagcatgg
     6061 acgtcattaa atgatcctga gcgtggacca gcaccagact cactttaatc ttaccttcgc
     6121 ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc ttcattcaat gccatccgtg
     6181 actgatccat catggctttt gctgcagcaa aatcgccctg cttcgcctgt ttcagtgccg
     6241 catacgccag gctgcgcgct tgtccggagt tgatgataag ccccatcacc acttcttcca
     6301 gctcttcagc ttctgtttgc gtatcaggaa tattatcgag atccatcata catcgttcct
     6361 ctctcttctt accggcacga ttacccgtac cggcatcgat taaaatttca gggcgttagc
     6421 gatatcttct tcgctttctt ctttatcaat cgcattttgt gctttgttag ccaccacaac
     6481 aaagggcaga taaattaacg ttgcgatgcc aaggttgaag agtgcgacca gcaatgcggc
     6541 gacgctaccg ttggtgttaa agaaggctcc cagaccggtt ggcatggtcc acggtgcaat
     6601 attggtcacc ggaggaataa tgcccatgta gtacgctgcg agggtgattg ccgccagaat
     6661 cggttgtacc agtacaaacg ggataaacat caccgggttc atgataattg gcagaccaaa
     6721 cagaatcggt tcgttaatct ggaagatgcc ggacggcagc gccagttttg ccacctgacg
     6781 ataatcagca cggcgagatg cgataaagat agccaggatc aggcctaaag tcgcaccact
     6841 gccaccgagg aaaataaagg agtccagcat cggcttggcc cagatatgga aggtcttacc
     6901 ggctgccagc gccgcttcga cggaaccata ttgctgatag gtcgcgatat tttccagcgc
     6961 ccacggcgtc ataatgccgt tgtccagtgc ggtcagcgcc agcgcgccat gaataccgaa
     7021 gaaccagagc agtggaacaa agatcacata ggcccagccc accacgctac ccaacgatgc
     7081 cagtggggtt gagatggtat ccataatgat ctgatggaag ttggtgcccc aggtattcaa
     7141 cgcccaggca ataatcccca tcacggaaag aataataaag ccgggaatta atgccgagaa
     7201 ggaacgcgat actgaagcag gtacgctgtc gggcagttta atgacccaat tgcggcggac
     7261 aataaaggta aacatttctg ccaccaccag gccaataata atcccggaga tgatattcgc
     7321 cccacctaac cagtttgcgc caaccgcata ggcctcaccg acactatatg gggtgacggt
     7381 cataaatgct gcaacggata acaacccggc cgccagcgca tcgactttac gctcttctgc
     7441 cagcgccatg ccaataaaga acggtgccat taaagacatt atccctaatg ttccgttata
     7501 tacgttgccg ccaatacctt tcagaccatt aagtgtttca atggttgagg cgtcgaggcg
     7561 aatacctaag gaataaaaaa acgacccctc cccaaagctt agaaaaacgt tgttaattaa
     7621 tacaaacatg gcccccgcaa gggttaacgg cattaagcga ataaagccat ttttgattgc
     7681 attaacgtgt ggctgctttc ctattttaac tgcaaaaggg aggagtacct tttca
//
LOCUS       DBANUU010000177         7675 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000177 DBANUU010000000
VERSION     DBANUU010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.180"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..329)
                     /locus_tag="ACIE68_004167"
     CDS             complement(<1..329)
                     /locus_tag="ACIE68_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFX5910391.1"
                     /translation="MNLEKGGRGAIERMVEAYGFKTRQALCDHLGISKSTLATRYMRD
                     SFPAEWVIQCALETGTSLNWLTTGHGSKQTSGNTNTMEVAKYVLSDGALCEDGFYIFD
                     REFLPSA"
     gene            455..676
                     /locus_tag="ACIE68_004168"
     CDS             455..676
                     /locus_tag="ACIE68_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFX5910392.1"
                     /translation="MRPNISITLTTPHVTIERYSELTGLSIDTINDMLADGRLIRHRL
                     RKDKKREKVMINIAAMTVDALSECNLNLN"
     gene            709..1218
                     /locus_tag="ACIE68_004169"
     CDS             709..1218
                     /locus_tag="ACIE68_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HFX5910393.1"
                     /translation="MFDYQVSKHPHFDESCRAFALRHNLVQLAERAGMNVQILRNKLN
                     PAQPHLLTAPEIWLLTDLTEDSTLVDGFLAQIHCLPCVPINEVAKEKLPHYVMSATAE
                     IGRVAAGAVSGDVKTSAGRRDAISSINSVTRLMALAAVSLQARLQANPAMASAVDTVT
                     GLGASFGLL"
     gene            1226..1522
                     /locus_tag="ACIE68_004170"
     CDS             1226..1522
                     /locus_tag="ACIE68_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage filamentation protein Fil family protein"
                     /protein_id="HFX5910394.1"
                     /translation="MLTKEPSFASLLVKQSPAMHYGHGWITGEDGKRWHPCHSQDELL
                     SELTTRKRRKSKCMRQKVKWFISFVTEGRVIQYLKMICSVAIRHCRAMAVTFSR"
     gene            1640..1981
                     /locus_tag="ACIE68_004171"
     CDS             1640..1981
                     /locus_tag="ACIE68_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5347 domain-containing protein"
                     /protein_id="HFX5910395.1"
                     /translation="MAINGAAATVPLSPGERLNGLNHIAELRAKVFGLNIESELERFI
                     KDMRDPRDINNEQNKRALAAIFFMAKIPAERHSISINELTTDEKRELIKAMNHFRAVV
                     SLFPRRLTMPN"
     gene            2049..2282
                     /locus_tag="ACIE68_004172"
     CDS             2049..2282
                     /locus_tag="ACIE68_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFX5910396.1"
                     /translation="MRNIETLTTKTGPDDAGLNILLTEARLEERRARAEAMAARLDSL
                     ACHITSRQLNHVEAAELLRVTAEAIQNEAQEIH"
     gene            2282..2509
                     /locus_tag="ACIE68_004173"
     CDS             2282..2509
                     /locus_tag="ACIE68_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFX5910397.1"
                     /translation="MADAMDLVQQRVEEERQRHIRAARAKTPGVSRVLCIECEAPIPP
                     ARRRAIPGVQLCITCQEIAELKGKHYNGGAV"
     gene            2506..3363
                     /locus_tag="ACIE68_004174"
     CDS             2506..3363
                     /locus_tag="ACIE68_004174"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - obsolete DNA methylation on
                     adenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFX5910398.1"
                     /translation="MSTILKWAGNKTAIMSELKKYLPAGPRLVEPFAGSCAVMMATDY
                     PSYLVADINPDLINLYKKIAADCEAFISRARVLFEEANSELAYYNIRQEFNYSTEITD
                     FMKAIYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAPRATFIC
                     ASFDETLAMLKAGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIV
                     SNSDTSLIRSLYRNFTHHYIKAKRSIGVSAGESKSATEIIAVSGARCWVGFDPSRGVD
                     SSAVYEVRV"
     gene            3360..5774
                     /locus_tag="ACIE68_004175"
     CDS             3360..5774
                     /locus_tag="ACIE68_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFX5910399.1"
                     /translation="MSHADMSDSSGFNEAAAAFSWNGPKKAINPYLDPAEVAPVSALS
                     NLITLYAADNEQEQLRREELSEQVWERYFFNESRDPVQREMEQDKLISRAKLAHEQQR
                     FNPDMVILADVSAQPTHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLDCVRD
                     SQLSTSFRFMASHQGLEGLLILPEMSQDQVKRLSTLVAAHMSMCLEAACGDLYTNDDV
                     KPEEIRKTWEKVAAETLRLDVIPPAFEKLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEDG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYLVGMFAAFRKAMHKTGLRWYGVRVAEPHHDGTVHWHLMCF
                     MRKKDRRAITALLRKFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLRDNAEYVNAWASLHRVQQFRFFGIPGRQAYRELRLLAGQAAR
                     QQGDKKAGAPVLDDPRLDAILAAADAGCFATYIMKQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHGIRIYGIWSPISEGKICTHAVKWKMVRKAVDVQEAVADQGACAPWTRGNNCP
                     LAENLYQQGKDKSADGGSRTDITRMDDKELHDYLHSMSKKDRRELAARLRLVKPKRRK
                     DYKQRITDHQHQQLVYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLREDD
                     KWRNLY"
     gene            5928..6116
                     /locus_tag="ACIE68_004176"
     CDS             5928..6116
                     /locus_tag="ACIE68_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910400.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRTHEYNRSMDVIS"
     gene            6127..6360
                     /locus_tag="ACIE68_004177"
     CDS             6127..6360
                     /locus_tag="ACIE68_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFX5910401.1"
                     /translation="MRIEIMIDKEQKISQSILDALESELYRNLRPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKQVMKIMQKVWEDDSWLH"
     gene            complement(6627..7157)
                     /locus_tag="ACIE68_004178"
     CDS             complement(6627..7157)
                     /locus_tag="ACIE68_004178"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910402.1"
                     /translation="MATTNCITVLTARGLTEILNTGGSQAWRLDASHAAKHEYLVCVQ
                     NSRKDWGSQEAKHHHAFMIGRISGVSRAPENPKRWIININSYAEIDIPGQWDGNRNPV
                     SYRNLKDIGIEADKLEFKALPKLTPTNSIVDQAGDEYEEEEENVVKPLTLQEAKAGLA
                     LYFGVNEENIQITIQG"
BASE COUNT         2037 a         1715 c         1945 g         1978 t
ORIGIN      
        1 aatgccgacg gtagaaattc tctatcgaaa atataaaaac cgtcttcaca caaggcccca
       61 tcagataata catatttagc aacttccata gtatttgtat tacctgaagt ttgctttgaa
      121 ccatgcccgg ttgtgagcca attaagcgag gtgcctgttt caagggcgca ctggattacc
      181 cattctgctg ggaatgagtc acgcatgtag cgtgtggcga gtgtactttt agagattcct
      241 aaatgatcgc acaacgcctg tcgagtcttg aatccataag cttctaccat gcgctctata
      301 gcgcctcgtc cgcctttctc caaattcatg gtcactccaa gtgaactttt atcttgacga
      361 tttcactgtg cgatcgtatg tttatggtgt tcacaaaata caaacgatcc gtattcgtcc
      421 tgattaatca tcattaaacg aggaatgttg catcatgaga cctaacattt caatcactct
      481 tactacacct catgtgacta ttgaacgcta tagcgagctg acagggctat ccatcgatac
      541 catcaatgac atgttggctg atggacgcct tatccgtcac cgtctgcgca aagataaaaa
      601 acgcgaaaaa gtgatgatca acatagcagc aatgaccgtt gatgcgcttt cagaatgcaa
      661 tctaaacctt aattagttcg attctgaaat acatcagagg cattgaccat gtttgattac
      721 caagtttcca aacatccaca ttttgatgaa tcctgtcgtg cattcgcatt gcgccacaac
      781 ctggtgcaac tggcagaacg tgctggcatg aatgtgcaga ttctgcggaa caagttgaac
      841 ccagctcaac ctcatttatt aaccgcacca gaaatctggc tgcttaccga tctgactgaa
      901 gattcaacgc tggtagatgg ttttctggct cagattcact gcctgccatg cgtaccgatt
      961 aatgaggtgg caaaagagaa actgccacat tacgtcatga gtgcaaccgc agagatcggg
     1021 cgtgttgctg caggtgcggt atctggcgat gtaaaaacca gtgcaggtcg tcgtgatgct
     1081 atcagcagca ttaactctgt aacacgactg atggcgttgg ctgctgtttc attgcaggcc
     1141 cgtttacagg ctaaccctgc gatggcgagt gcagttgata ccgtgactgg cctcggtgct
     1201 tcatttggtt tgctgtgagg tgcttatgct gacgaaagaa ccatcatttg catcgctgct
     1261 ggtaaaacaa agcccggcaa tgcactacgg tcacggctgg atcacgggtg aggatggaaa
     1321 acgctggcat ccatgtcatt cacaagatga attgctgtct gaattgacca cgaggaaacg
     1381 gagaaagtcc aaatgtatgc ggcagaaagt gaagtggttt atcagtttcg ttacagaggg
     1441 gagagttatt cagtacctga agatgatttg ctctgttgct atccgtcatt gtcgggcgat
     1501 ggcagttact ttttcacgtt aaaggatggg acgtttttac ggggagagca ggttaaagag
     1561 acgatacgaa aaaatgtatc tcctcttgag cgttaccgta agaacaaaga acgatagctg
     1621 cgtttggggg atatgaagta tggcaattaa tggcgctgca gcgactgttc cattaagccc
     1681 cggtgaacgc ctgaatggac ttaatcacat tgcggagtta agggcgaaag tatttggcct
     1741 gaatattgag tcagagcttg agcggtttat taaagatatg cgtgatccac gggatatcaa
     1801 taatgaacaa aataaacggg cactggctgc catattcttt atggcaaaaa ttccagctga
     1861 acgtcatagc atcagcatta atgagctgac cactgacgaa aagcgggagt tgattaaagc
     1921 aatgaatcat tttcgtgcag tggtgagctt atttcccaga cggctaacca tgccgaatta
     1981 accaactaat gaaattaatg gcgtaaaccc gccgggcatc cctttatcta aattcaggag
     2041 aattgattat gcgtaatatt gaaaccctca cgactaaaac cggaccggat gacgcagggc
     2101 ttaatatttt actgacagag gctcgtctgg aagaacgccg ggcaagggct gaagcaatgg
     2161 ctgcccgcct tgatagtctt gcgtgtcata tcacatcccg ccagctaaac cacgtcgaag
     2221 cggcagaact gctgcgtgtg accgctgaag caatccagaa cgaagcgcag gagatccact
     2281 aatggctgat gcaatggatc tcgtacagca gcgcgttgaa gaagaacgcc agcgccatat
     2341 ccgtgctgcc cgtgccaaaa caccgggcgt gtctcgcgtg ctttgtattg aatgtgaagc
     2401 gccaattccg ccagcacgcc gccgcgccat tcctggagtg cagctttgca ttacctgcca
     2461 ggaaatcgca gagctgaaag gcaaacatta caacggaggt gctgtatgag caccatcctg
     2521 aaatgggcgg gtaataaaac cgccattatg tccgaactga aaaaatacct tcctgctggc
     2581 ccgcgactgg ttgaaccttt cgcgggttct tgtgctgtga tgatggcgac ggattacccc
     2641 agctatctgg ttgcggatat taatcctgat ttaatcaacc tctataaaaa gattgccgct
     2701 gattgcgaag cgtttatatc tcgtgccaga gttttatttg aggaagcaaa cagtgagctg
     2761 gcttattaca acataagaca ggagtttaat tactcaactg aaattactga tttcatgaag
     2821 gcgatatatt tcctgtatct caatcgtcac ggttaccgtg gtttatgtcg ctataacaag
     2881 agcgggcatt tcaacattcc ctacggtaat tataaaaatc cgtatttccc tgaaaaagaa
     2941 attcgcgcat ttgcagagaa agccccgcgg gcaacgttta tctgcgccag ctttgatgaa
     3001 acgctggcga tgttgaaggc gggggatgtg gtgtattgcg atccgccgta tgacggtacg
     3061 ttttccggct atcacactga tggtttcact gaagatgacc agtatcacct ggcatccgtt
     3121 cttgaacatc ggtcatcaga aggtcatccg gtcattgttt ctaacagtga cacatccctg
     3181 attcgttcgc tgtatcgcaa tttcactcac cactacatca aggcaaaacg cagcatcggc
     3241 gtgtcggctg gcgagagtaa atctgcaaca gaaatcattg ctgtttccgg ggcgcgctgc
     3301 tgggtgggat ttgatccttc gcgtggcgtg gatagttctg ctgtgtacga ggtgcgtgta
     3361 tgagtcatgc cgatatgagc gactctagcg gctttaacga ggccgctgca gcattttcat
     3421 ggaacggccc gaaaaaggcc ataaaccctt atctggaccc ggcggaagtt gcgccggttt
     3481 ctgcgctttc aaacctgatc actctgtatg ctgccgataa cgagcaggaa cagctgcgcc
     3541 gcgaggaatt gagtgaacag gtctgggagc gttatttctt taatgaatcc cgtgatcctg
     3601 tccagcgcga aatggagcag gataagctca ttagtcgggc aaagctggcg catgagcagc
     3661 agcgttttaa cccggacatg gtcattctgg cggacgtcag cgcccagccc acccacatca
     3721 gcaagccgct gatgcaacgt atcgaatact tcagcagcct gggcaggcca aaggcttatt
     3781 cccgctattt gcgtgagacg attaagccat gtctggagcg actggattgt gtacgcgaca
     3841 gtcagctatc tacttctttc cgttttatgg caagccatca agggctggag ggcctgctga
     3901 tcctgcctga aatgagtcag gatcaggtga aacgcctgtc caccctggta gctgcgcata
     3961 tgagcatgtg tcttgaggcc gcttgtggtg atttgtatac caacgatgac gtaaagccag
     4021 aagaaatccg caagacatgg gaaaaggtgg cagcggaaac cctgcgtctg gatgttatcc
     4081 cgcctgcgtt tgagaaactt cgtcggaaaa gaaaccgccg caaacctgtg ccctatgaac
     4141 tcataccggg ttcgctggca cgtatgcttt gcgccgactg gtggtatcga aaattgtgga
     4201 agatgcgttg cgaatggcgg gaagagcagt tgcgtgctgt ctgtctggtc agcaaaaaag
     4261 catctcccta tgtcagctat gaagccgtga tgcataaacg tgagcagcgc cgcaagtcgc
     4321 tggagttttt ccgttctcat gaactggtga acgaagacgg agacacgctg gatatggaag
     4381 atgtggtaaa cgccagcagc agcaacccgg cacatcgccg caatgagatg atggcctgcg
     4441 ttaaaggtct ggagcttatc gcggaaatgc gcggtgactg cgccgttttc tacaccatca
     4501 catgtccgtc acgtttccat tccacgctca ataacggcag gcccaacccg acctggacaa
     4561 atgcgacggt aagacaaagc agcgattatc tggtcggcat gtttgctgca tttcgtaagg
     4621 cgatgcacaa aaccgggttg cgctggtatg gcgtacgggt ggctgagccg caccatgacg
     4681 gtactgtgca ctggcatctc atgtgtttca tgcgcaaaaa agaccgccgc gccattactg
     4741 cattgttgcg taagtttgcc atccgtgaag accgcgagga gctgggcaat aacacgggcc
     4801 cacgctttaa gtctgagctg ataaacccac gcaaaggaac gccgacaagc tacatcgcaa
     4861 aatacatcag taagaacatt gacgggcgtg gtctggctgg cgagatcagc aaggaaacgg
     4921 gtaaatccct gcgtgataac gccgaatacg ttaatgcctg ggcgtctctg catcgtgttc
     4981 agcagttccg cttctttggt attccggggc gccaggctta ccgtgaactg cgattgctgg
     5041 ctggtcaggc ggcaagacaa cagggtgaca aaaaagcagg tgcgccggta ctggatgatc
     5101 cgcgtcttga tgccatcctg gctgctgctg atgctggttg ttttgccacc tacatcatga
     5161 agcagggcgg cgtactggtt ccccgcaaat atcaccttat cagaacagct tatgaaatta
     5221 acgaagagcc gactgcctat ggcgatcacg gtattcgtat ttatggcatc tggtcaccca
     5281 tttcagaggg caagatctgc actcatgcag tgaagtggaa aatggttcgt aaggccgttg
     5341 acgttcagga ggcggtagcc gaccagggcg cttgcgcccc ttggactcgt ggcaataact
     5401 gtccccttgc tgaaaatttg taccaacaag ggaaagacaa atcagctgat gggggctcca
     5461 gaacggacat tacccgcatg gatgacaagg aattgcacga ttacctgcac agtatgagca
     5521 aaaaagatcg ccgggagctg gcagcaaggt tgcgcttggt gaaaccgaaa cggcgtaaag
     5581 actacaaaca gcgaattaca gatcatcagc atcagcagct cgtctatgaa ctgaagtcca
     5641 gaggatttga tggcagcgag aaagaagtcg atttgctcct tcgcggtggc agtattccgt
     5701 caggagcagg cttgcgtatc ttctatcgga accagcgttt gcgggaagat gataagtggc
     5761 ggaacctgta ttaattacgc gggttaacaa ttcgtgctct taataatacc aggcatatca
     5821 ggctgatgaa cgtaaaaaaa cgttttacat cagtaagatt attatatact gtaaatataa
     5881 acagtggtta tgtatacagc atttcttgtg gtgtcatagg aggaaagatg caggactatt
     5941 ttttggagtc tttgaagctc cagcgcattg atttttttct taagcttgta gcggctagtg
     6001 agtgtagtga tgaagagaag gggctggctt tgcagtgggt ttctgaacta acagatgaac
     6061 tcatggcaaa aatcagaacc cacgaataca accgctcaat ggatgtcatc agttgaggtg
     6121 acttttatgc gcattgaaat aatgatcgat aaagagcaga agattagcca gtctatcctg
     6181 gacgcccttg aatccgagct ttaccgcaat ctgcgccccc tgtatcccaa aacggtaatt
     6241 cgtatccgca aaggtagctc taacggtgtg gaactgaccg gactgcaact ggacgaagaa
     6301 agaaaacaag tgatgaaaat tatgcagaag gtgtgggaag acgacagctg gctgcattaa
     6361 gaaacgttgc tggcgtctga acttgcttct gacgtcagca acgttgaaca actagtttag
     6421 tggggcatta gtcatggggt aaaatacagt aaccattatt ttcacttccg ctcagattta
     6481 accgaacgtt agttttgcta cgtatgatgg gctgcaaatt accttttggg atttgtgata
     6541 gatacgaaaa tcctattaga tgcttgttag accggaagcg ccacttcgaa cgaaaagtaa
     6601 tggccactta tgccggttta aaagccttaa ccttgtatag ttatttgaat attttcttca
     6661 ttgacaccaa agtatagggc tagccctgct tttgcctcct gaagtgttaa cggcttaact
     6721 acattttctt cttcttcttc gtactcatca cccgcttgat cgacaatcga attagtcggg
     6781 gttaattttg gaagagcttt aaattccaac ttgtcagcct caatgccaat gtctttgagg
     6841 ttgcgataag aaacgggatt ccgattgcca tcccattgtc ctgggatgtc aatttcagca
     6901 taagaattga tgtttatgat ccagcgttta gggttttctg gagccctgct tactcccgag
     6961 attcgaccga tcataaaagc atgatgatgt tttgcttctt gtgatcccca gtcctttctc
     7021 gaattttgta cacaaactaa atattcgtgc ttagctgcat ggctggcgtc aagtctccat
     7081 gcttggcttc cccctgtgtt caaaatctct gttaaaccac gagcagtaag tacagtaata
     7141 cagttggtag tagccattga attgtgaccc cgtctttttt agagaaatac tattgatgaa
     7201 tgtagtttac gtattttttt attgagtagt caagttagta ttctgctcgt tattggctgt
     7261 tttttgagtg ttgtgagtta gtactttgtg tagtacctct tggtttatga gggttaaatc
     7321 taagttatga caaagggagg cagggggaac gcgcagcgca aggtgtacag gaggccataa
     7381 ggactcactt gaagtaagaa aagatcgata aagcaacaat gcatgtctat gccgcatgaa
     7441 ttcgcatgat cgtttgagga tcgttttagc tgaggcccgc taggaatggc gggcttttgt
     7501 ttatgtcatg caagtgcatg aaaaccacta cataaagcgg gcaggcgtgg cggggatacg
     7561 agcgcgcgct gggaggtata agaatgaagt acaattaact tcactttgtt gagtaaaatg
     7621 ctataagaat aatgcaaatt attgagtccc attagttcaa aacttaggga tatct
//
LOCUS       DBANUU010000178         7671 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000178 DBANUU010000000
VERSION     DBANUU010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.181"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..137)
                     /locus_tag="ACIE68_004179"
     CDS             complement(<1..137)
                     /locus_tag="ACIE68_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HFX5910403.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVTTAGLAWVAKYL
                     E"
     gene            complement(342..1046)
                     /gene="ybhL"
                     /locus_tag="ACIE68_004180"
     CDS             complement(342..1046)
                     /gene="ybhL"
                     /locus_tag="ACIE68_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFX5910404.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1183..1635)
                     /gene="moaE"
                     /locus_tag="ACIE68_004181"
     CDS             complement(1183..1635)
                     /gene="moaE"
                     /locus_tag="ACIE68_004181"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFX5910405.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1637..1882)
                     /gene="moaD"
                     /locus_tag="ACIE68_004182"
     CDS             complement(1637..1882)
                     /gene="moaD"
                     /locus_tag="ACIE68_004182"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFX5910406.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1875..2360)
                     /gene="moaC"
                     /locus_tag="ACIE68_004183"
     CDS             complement(1875..2360)
                     /gene="moaC"
                     /locus_tag="ACIE68_004183"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFX5910407.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2363..2875)
                     /gene="moaB"
                     /locus_tag="ACIE68_004184"
     CDS             complement(2363..2875)
                     /gene="moaB"
                     /locus_tag="ACIE68_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFX5910408.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2897..3886)
                     /gene="moaA"
                     /locus_tag="ACIE68_004185"
     CDS             complement(2897..3886)
                     /gene="moaA"
                     /locus_tag="ACIE68_004185"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFX5910409.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3874..4018)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4283..5191
                     /gene="yvcK"
                     /locus_tag="ACIE68_004186"
     CDS             4283..5191
                     /gene="yvcK"
                     /locus_tag="ACIE68_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFX5910410.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5383..7404)
                     /gene="uvrB"
                     /locus_tag="ACIE68_004187"
     CDS             complement(5383..7404)
                     /gene="uvrB"
                     /locus_tag="ACIE68_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFX5910411.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         1858 a         2024 c         1908 g         1881 t
ORIGIN      
        1 atttcgagat acttcgctac ccaggccagt cctgcggttg tcaccaaacc taaagcggta
       61 atgagccata taccgtttaa tatcctggtc tcgtgctgaa aatctaattt attgctgttt
      121 tgtgagtatg attccattct tttagttgtc cttagaaaaa atattaatat tcaccatcaa
      181 taaatttaaa accacgcata caaaatactt ttgtatttat ggatacggaa aacgcttccg
      241 ctcgccataa ggcgtatttt atacctggta cttgttaagt gtcgttttta gtaagaaaaa
      301 aaatcccgac acatggccgg gatattgatg gtgaaaaaga attaacggcg gttgccaaaa
      361 attcgcagca acatcaggaa caggttgatg aagtccagat acaaggttaa cgcgccaaga
      421 atggaatatt tgcgcaggtt cgacgtgtcg cgggtatcaa tctgctcacc catatttttc
      481 aatttctgag tgtcatacgc cgtcaatccg acaaagacaa tcacgccgat gtaggtaact
      541 gcccacatca atgcttcgct tttcagccag aagttgacca gcgatgccag cacaatgccg
      601 attaacgcca taaacagcat attgccaaag ccacttaaat cgcgcttcgt ggtgtaaccg
      661 tacaggctca ttgcgccgaa catcccggca gtaacgacga aagtactggc gatagaagca
      721 gcggtataga caatgaatat actggaaagc gtaagacccg tcagcgccga ataaagcata
      781 aagagcatcg tcgttacacc tgcgctcagc ttttgaatca tcgctgataa cacaataacc
      841 aatgctaatt gcgcgatgat cagaccgatt aaaaagacac ggttagtaaa caacagctcc
      901 atcacggccg cggaattagc cgcataccag gcaacaaatg cggtcagcaa caagccaacg
      961 gtcatccagc catagacttg cgccatataa gtttgcaagc cagcccgggg ttgtacgatt
     1021 gaatcagaac gtgggaatct gtccatgacg atctcctgaa gatataagga atatcttaag
     1081 gatactgcaa aatgatgagg ctgtgcatcg acgcagcgta aacgcatgta ctgagcggtg
     1141 aaattgccgg acgcagcggt gccttatccg gctaacaaaa aactaccagc gttttgccgc
     1201 ctgctgatcg ctctcccgag cttcaaccca gcggtcgcct tccggtgtgg cttcgcgctt
     1261 ccagaacggt gcgcgggttt tgagataatc cataataaac tgcccggctt caaacgcact
     1321 gctgcgatgc gcactggtga caccgacaaa aacgatttca tcgcccggcc ataattcccc
     1381 gatgcggtga atcacagtga cgcgccccag cggccagcgg ttacgcgctt catcaacaat
     1441 ttctgccagt gctttttcag tcatccccgg atagtgttcg agggttaatg ctttgacgct
     1501 gtcgcccagg ttatggttgc gcaccttacc agtaaaggtg actaccgcac cgtcttcgtc
     1561 acgctccgcc agccacgggt actcttctcc tacgctgaac ggctgcggac caacaacaat
     1621 tttggtttct gccatcttaa cctccggtta ccggcgggaa gaaagctact tcgtcgccgt
     1681 cagtcagcgg atggtcaaaa ctcaccagcg tctggttgac ggcagccagt aatttgccat
     1741 cttccagcgc cagcgcccag cgatcgctct gcgcagccat gtgctggcgt aacgcttcaa
     1801 cagttgggaa atccgcagcc acttcggttg catctgttcc caccaactcg cgcacctggg
     1861 cgaaaaaaag aactttaatc atccgcttcc accttaaagt cacccgactt gccgccgctc
     1921 ttcgccagca aacgtaccgg accaatcacc atatcttttt gcaccgcttt gcacatgtca
     1981 taaatggtca gcgccgccac ggaggccgcg gttaatgctt ccatttcgac accggttttc
     2041 ccggtcaggc ggcataaggt ttctatacgc acccgattgt gctccggctc ggcctgtaaa
     2101 ttgacttcaa ctttgctgag catcagcgga tgacagagcg ggatcagatc ccaggtgcgt
     2161 tttgccgcct gaataccggc aatacgcgca gtggcaaata cgtcgccttt gtggtggcga
     2221 ccatcaataa tcatcgccag cgtctcgctg cgcatggtga caaaggcttc cgcccgcgct
     2281 tcacgcacgg tttccgcttt ggcggagaca tccaccatgt gcgcttcgcc agcggcgttg
     2341 atatgggtca gttgcgacat acttatttct tcaaatgtgg atggaaatta cacggacgcg
     2401 tacgggcatc cagctgcggc gcgatgatat tttcccatgc ggtacggcac gctttggtcg
     2461 aacccggcat ggcgaaaatc agcgttttgt tggcgacgcc cgctaccgca cgagattgca
     2521 acgtggaagt gccaatctct tcaaacgaca acatacggaa cacttcacca aaaccttcaa
     2581 tttcacggtc gaacaacggc agcaatgctt cgggagcctg atcaccttcc gtcaggccag
     2641 taccacccgt aatcaatacc acttgtacat cgtcgctggc gatccacgca gatacctgag
     2701 cgcgaatagc gtagcggttt tctttcacaa tggctttatc gacaacgtga tggcccgctt
     2761 cttgcgccga atcgcgcaga tagtgaccgg aggtatcgtc ttcttcaccg cgacgattag
     2821 aaaccgtaag aatagcaata cgggtcggga taaattcagt gcttacctga ctcatctgat
     2881 ctctcctttt gacgttttag ccgccaatgt acgataagtt ttgcgtaata ccggtgttgt
     2941 tttgatgcag gaaatgggtc tgtttcttct cccgcagcgc cgctgaaata cgcgcttcca
     3001 gcgcctgttg ctgggtatcg tcttccagca gatcgcgcag gttaacgccg ccttcaccaa
     3061 acaggcagag atggagttta ccaatggagg aaacgcgcag gcggttgcaa gtggcgcaga
     3121 agtctttttc atacggcatg ataaggccaa tctctccggc gtaatctgga tggcaaaaga
     3181 cttgcgcggg accgtcgctg cgttgacgta attggtggat ccagccgcga cgcagtagct
     3241 cgtcacgcag aacctgacca gagatgtgat gcttacggaa gagctcgctg ccctcgcccg
     3301 tttccatcag ttcgatgaaa cgcagctgga tagggcgatg ctggatccag ttcagaaagg
     3361 tgtcgagctg gtgatgatta acatcacgca tcagcacggt attgactttg accttctcaa
     3421 aaccggcctc aaatgcagca tcaatccccg ccatgacctg gttgaattta tcctgcccgg
     3481 taatagcgtg aaactggcgg gcgtccagac tgtcgacact gacgttaatg ccagtaagtc
     3541 ccgcatcgcg ccagttcgcc acatcgcgtt ccagacggta accattggtg gtgaccgcaa
     3601 tctggcggat agcgtcgttt tcccgcacag cggcgatgat atcggtaaag tcgcggcgta
     3661 aagacggctc accgcctgtc aggcgcactt tttcggtgcc cagactggcg aaggcgcgcg
     3721 taacccggcg aatttcatcg acggtaagaa agcctttatt ggtgacgccg ctcggtttgt
     3781 agccatccgg caggcagtag gtgcaacgaa agttacacac atcggtaatc gacaggcgca
     3841 agtagtaaaa cttacgcgca aatgcatcag tcagttgtga agccatgtac acctttccag
     3901 atacgggagg cgaagtcatt tcttccttcg ccctggtggc aatctttacg agcattgtca
     3961 cggccaaagc accgtatcag ttgacccagg tgcagaggct agagtgttta gtggttatgc
     4021 cgatactagc gtgtaaatga gatttttacc atccacactt tcgctatgta atcatgtata
     4081 tagcgtcatg atcgtgctat ttcgctacag aataagggaa aatggcggct ttactttgat
     4141 atacatcatc agggaggcat agcgtgcatc gtcctttaga ggtagaagaa aatagtttgt
     4201 atcctcaata ttggcaggtt aattgctgtt tccccgcaat ttgcgctact gtagcgcgga
     4261 taatctgact ccaggaaact atatgcgcaa tcgtacgctg gctgatcttg atcgtgtcgt
     4321 tgctctcggc ggagggcatg gactgggacg cgttctctca tcactttcgt ctttgggttc
     4381 tcgtttaacg ggtatcgtca ccaccaccga taatggtggc tcgacggggc gtattcgccg
     4441 ttcagaaggc ggcattgcct ggggcgatat gcgcaactgc ctcaaccagc tgataacgga
     4501 accgagcgtc gcctccgcga tgtttgaata ccgttttggt ggcaatggcg aactttccgg
     4561 tcataatctc ggaaacttga tgttaaaggc gctggatcac cttagcgtgc ggcctctgga
     4621 agccatcaat ttaattcgta atctgctgaa agtggatacg catttgattc caatgtcaga
     4681 gcatcctgtt gatctgatgg cgattgacga tcaggggcat gaagtttacg gcgaggtcaa
     4741 tatcgaccag ttaactacgc cgattcaaga gttattgtta acgcctaatg tacccgcaac
     4801 gcgtgaggcg gttcacgcta tcaatgaagc ggatctcatc attattgggc ctggcagttt
     4861 ttataccagc ctgatgccaa ttctgctgct gaaggaaatc gcccaggcat tacgccgcac
     4921 gccagcgccg atggtttata tcggcaatct ggggcgtgag ttgagtttac ctgcggctaa
     4981 tttgaagctg gaaagcaagc tggcaattat ggagcagtat gttggtaaaa aggtcattga
     5041 tgcggtcatc gtcgggccaa aagtggatgt ctcggcggtg aaagagcgga ttgtgatcca
     5101 ggaggtactg gaggccagcg atattccgta tcgtcatgac cgccagttgt tacataacgc
     5161 gctggaaaag gcgttacagg ctttaggtta acggaaatcc gaaggaaaat tccggcttcc
     5221 tattgaagac aaagtgcgcg ttgtttatgc cggatgcggc gtgaacgcct tatccggcct
     5281 acaaaccgcg caaattcaat atattgcgga gaaaatgtag gcctgataag cgtagcgcat
     5341 caggctgttt tccgtttgtc atcagtcttc ttcgctatcc tgttacgatg ccgcgataaa
     5401 cagctcacgc agctgatgca actggtcacg aatttgcgcc gcttcttcga actccagatt
     5461 ctgcgcgtgt tgcatcatca acccttccag ctcatggatt ttctgctgca acgctttagg
     5521 cgacatatcc atcggcacat tatccggctc aacaatcggt cgcgattttc ctctgccctt
     5581 cgctttggtt ttagcaatgt tctgccccaa cgccaggata tcgaccactt tcttgttcaa
     5641 gccttgcggc gtaatgccgt gttcctcgtt gtacttctgc tgtttctcgc ggcgacgttc
     5701 ggtttcgcca atcgctttcg ccattgatgg ggtgatctta tcgccgtaga gaatcgcttt
     5761 accgttaacg ttacgtgccg cacgaccaat ggtctggatc aacgaacgtt cggaacgcag
     5821 gaagccttct ttgtcagcgt cgaggatcgc caccagcgaa acttccggca tatccagacc
     5881 ttcacgcagt aagttgatcc ctaccaatac gtcgaactca cccagacgca agtcgcggat
     5941 aatctccata cgctcgacgg tgtcgatatc tgagtgaaga taacgcacgc gctcaccgtg
     6001 ttcttcgaga tattcggtaa gatcttccgc cattcgcttg gtcagtgtgg tgaccagtac
     6061 gcgttcgtta attgccgctc gctgacgaat ctccgaaaga agatcatcaa cctgtgtcgc
     6121 caccggccgc acttcgataa tcgggtcaag caatccggtt ggacgcacca cctgatccac
     6181 cacatcgccg ccggattttt ccagctcgta attacccggc gtcgccgaaa catagatggt
     6241 ttgcggcgct aatgcttcga actcttcaaa cttaagcgga cggttatcca gcgctgatgg
     6301 caggcggaag ccgtactcca ccagtgtctc tttacgcgcc cggtcaccgc gatacatgcc
     6361 gccaatttgt ggaatggtga cgtgagattc atcgaccacc agcagcccat cagcagggag
     6421 gtaatcaaac agcgtcggcg gtggctcacc cggtccacga ccggagagga agcgcgagta
     6481 gttttcaatc cccgaacagt agcccagctc gttcatcatc tccagatcaa actgggtacg
     6541 ctgggtcagc cgctgctctt ccagcagttt gttgttttcc aacagcactt tgcgtctggc
     6601 ggccagctct tctttgatct cctccatcgc ctgtacgatg cgctcgcgcg gtgtgacgta
     6661 gtgcgttttc gggtagatgg taaaacgtgg aatagtggaa acaatctgcc cggtcagcgg
     6721 gtcaaataac gacaatcgtt ccacttcctc gtcaaacagt tccacgcgaa gtgcaatgtc
     6781 atccgattct gccgggaaga tatctatcac ctcgccacga acgcggaaag taccacgctg
     6841 gaatgcttga tcattacgag cgtattgcag ctccgccagt cggcgcagaa tcgcgcgctg
     6901 atcgataatc ataccgaccg tgagatggag catcatcttg agatataaat caggatcgcc
     6961 cagaccataa atcgcggaaa cagacgccac cacaaccaca tcacgccgct ccagcatcgc
     7021 tttggtggcg gacaaacgca tctgctcaat atgttcgtta accgaggcat ctttctcaat
     7081 gaaagtgtcg gaactcggta cataggcttc cggctgatag tagtcgtagt aggaaacgaa
     7141 atattccacc gcgttttccg ggaagaactc tttcatttcg ccatacagct gggccgccag
     7201 cgttttgttg ggcgcaagta ccatggttgg gcgctgaagg tcagcaatga cattggcaat
     7261 ggtgaaggtt ttcccggagc cagtcacgcc aagtaacgtc tggtgcgcca ggccatcttc
     7321 cagcccctct tcgagacgtc gaatcgcctc tggctgatcg ccagaaggtt taaaagcgga
     7381 attcagtttg aacggtttac tcatgagtcg ctacctgaag gagttgggcg ggcaggtatg
     7441 taattttact cgtcgtactt aattatgcca acaaattata ctggataaaa aaacagttca
     7501 tcaccataat atttctgtta cagcgtaaac tccgctgtca atcatgagca aaatttactc
     7561 tgtggcgaga taaaactccg gccttaccgg gttatcccca aagcaacggc ttttttaaca
     7621 tttgtcaaga tgagtgatga cagttttatg gcaagagatg cctgttcagt g
//
LOCUS       DBANUU010000179         7600 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000179 DBANUU010000000
VERSION     DBANUU010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.182"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            86..736
                     /gene="csgD"
                     /locus_tag="ACIE68_004188"
     CDS             86..736
                     /gene="csgD"
                     /locus_tag="ACIE68_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFX5910412.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            741..1130
                     /gene="csgE"
                     /locus_tag="ACIE68_004189"
     CDS             741..1130
                     /gene="csgE"
                     /locus_tag="ACIE68_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFX5910413.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1155..1571
                     /gene="csgF"
                     /locus_tag="ACIE68_004190"
     CDS             1155..1571
                     /gene="csgF"
                     /locus_tag="ACIE68_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFX5910414.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1598..2431
                     /gene="csgG"
                     /locus_tag="ACIE68_004191"
     CDS             1598..2431
                     /gene="csgG"
                     /locus_tag="ACIE68_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFX5910415.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2495..2986)
                     /gene="ycdZ"
                     /locus_tag="ACIE68_004192"
     CDS             complement(2495..2986)
                     /gene="ycdZ"
                     /locus_tag="ACIE68_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFX5910416.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3088..3642)
                     /gene="ycdY"
                     /locus_tag="ACIE68_004193"
     CDS             complement(3088..3642)
                     /gene="ycdY"
                     /locus_tag="ACIE68_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFX5910417.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3666..4403)
                     /gene="ycdX"
                     /locus_tag="ACIE68_004194"
     CDS             complement(3666..4403)
                     /gene="ycdX"
                     /locus_tag="ACIE68_004194"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFX5910418.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4458..5396)
                     /gene="ghrA"
                     /locus_tag="ACIE68_004195"
     CDS             complement(4458..5396)
                     /gene="ghrA"
                     /locus_tag="ACIE68_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFX5910419.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGACLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGEKVCGQVDRARGY"
     gene            5630..5717
                     /locus_tag="ACIE68_004196"
     tRNA            5630..5717
                     /locus_tag="ACIE68_004196"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5664..5666,aa:Ser,seq:gga)
     gene            5762..5898
                     /locus_tag="ACIE68_004197"
     ncRNA           5762..5898
                     /ncRNA_class="other"
                     /locus_tag="ACIE68_004197"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(6091..7077)
                     /gene="ycdU"
                     /locus_tag="ACIE68_004198"
     CDS             complement(6091..7077)
                     /gene="ycdU"
                     /locus_tag="ACIE68_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFX5910420.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEYI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRCLWHNDFMMACDMYFRWGHFNK"
     gene            complement(7074..>7600)
                     /locus_tag="ACIE68_004199"
     CDS             complement(7074..>7600)
                     /locus_tag="ACIE68_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFX5910421.1"
                     /translation="VKGINREQLQTMITESGIALDISCDNTPRQQVIGGTQASLNEFA
                     TLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYLAGLDIASPEHQVLMNVTAKSEVA
                     PSIIKENLSLHLTHTVKWTESLDTFLNMPTPVAFLEISNKPYLGNMLNDFAGVDQQRV
                     MHCRKAFSDAKVFK"
BASE COUNT         2140 a         1876 c         1710 g         1874 t
ORIGIN      
        1 gtgcaacatc tgtcagtact tctggtgctt ctattttaga ggcagctgtc aggtgtgcga
       61 tcaataaaaa aagcggggtt tcatcatgtt taatgaagtc catagtattc atggtcatac
      121 attattgttg atcactaaac cgtctttgca agcgacagct ctcttgcagc accttaaaca
      181 atcgctggca attacaggaa aattacataa tattcaacgt tctctggacg atatatcttc
      241 aggctctatt attcttctgg atatgatgga agcagataaa aagcttatcc attattggca
      301 ggatactttg agcaggaaaa acaacaatat caaaatattg ttgctaaata cgcctgaaga
      361 ttacccgtac cgcgacattg aaaactggcc tcatatcaac ggcgtttttt atgccatgga
      421 ggatcaagaa cgtgttgtca atgggttgca aggcgtcctg cgcggcgaat gctactttac
      481 gcaaaagctt gccagttacc tgattacaca ttctggtaac tatcgttata acagcacgga
      541 atcagccctc cttactcatc gggaaaaaga gatcctgaat aagctgcgta tcggcgcgtc
      601 taataacgag atcgctcgtt cgttgttcat cagcgaaaat acggttaaaa cgcatcttta
      661 taatcttttc aagaagatag ccgtaaaaaa ccggacacaa gcggtttcct gggcaaacga
      721 taacctcagg cgataaagcc atgaaacgtt atttacgctg gattgtggcg gcagaatttc
      781 tgttcgccgc agggaatctt cacgccgttg aggtagaagt cccgggattg ttaactgacc
      841 atactgtttc atctattggc catgattttt accgagcctt tagtgataaa tgggaaagtg
      901 actatacggg taacttaacg attaatgaaa ggcccagtgc acgatgggga agctggatca
      961 ctataacggt caatcaggac gttattttcc agactttttt atttccgttg aaaagagact
     1021 tcgagaaaac tgtcgtcttt gcactgattc aaactgaaga agcactaaat cgtcgccaga
     1081 taaatcaggc gttattaagt acgggcgatt tggcgcatga tgaattctaa ataaaaaatt
     1141 gttcggaggc tgcaatgcgt gtcaaacatg cagtagttct actcatgctt atttcgccat
     1201 taagttgggc tggaaccatg actttccagt tccgtaatcc aaactttggt ggtaacccaa
     1261 ataatggcgc ttttttatta aatagcgctc aggcccaaaa ctcttataaa gatccgagct
     1321 ataacgatga ctttggtatt gaaacaccct cagcgttaga taactttact caggccatcc
     1381 agtcacaaat tttaggtggg ctactgtcga atattaatac cggtaaaccg ggccgcatgg
     1441 tgaccaacga ttatattgtt gatattgcta accgcgatgg tcaattgcag ttgaacgtga
     1501 cagatcgtaa aaccggacaa acctcgacca tccaggtttc gggtttacaa aataactcaa
     1561 ccgattttta agccccagct tcataaggaa aataatcatg cagcgcttat ttcttttggt
     1621 tgccgtcatg ttactgagcg gatgcttaac cgccccgcct aaagaagccg ccagaccgac
     1681 attaatgcct cgagctcaga gctacaaaga tttgacccat ctgccagcgc cgacgggtaa
     1741 gatctttgtt tcggtataca acattcagga cgaaaccggg caatttaaac cctacccggc
     1801 aagtaacttc tccactgctg ttccgcaaag cgccacggca atgctggtca cggcattgaa
     1861 agattctcgc tggtttatac cgctggagcg ccagggctta caaaacctgc ttaacgagcg
     1921 caagattatt cgtgcggcac aagaaaacgg cacggttgcc attaataacc gaatcccgct
     1981 gcaatcttta acggcggcaa atattatggt tgaaggttcg attatcggtt atgaaagcaa
     2041 cgttaaatcg ggcggtgttg gggcaagata ttttgggatc ggtgcagaca cgcaatacca
     2101 gctcgatcag attgccgtga acctgcgcgt cgtcaatgtg agtaccggtg agatcctctc
     2161 ctcggtgaac accagtaaga cgatactttc ctatgaagtt caggccgggg ttttccgctt
     2221 tattgactac cagcgcttgc tggaagggga agtgggttac acctcaaacg aacccgttat
     2281 gctgtgtctg atgtcggcta tcgaaacagg ggtcattttc ctgattaatg atggtatcga
     2341 ccgtggtctg tgggatttgc agaacaaagc agaacggcag aacgacattc tggtgaaata
     2401 ccgccatatg tcggttccac cggaatcctg acagataaaa aagccggaat tatcatccgg
     2461 cattttactg tttaagcgaa agcgtgagtg cccctcacgc tttatttttg atttcctgct
     2521 cacggtgagc cgtctttgca ctacgcgccg ccagccacag gccgctgttt ttcattgcgt
     2581 aaccaaatac cagcccaagc gccagcgaag gtaacaccag tttccagtca ccctgcccgg
     2641 caaacgtcgc gcatgcgcct ataaacgtcc ccggaacaaa tgaaagcagc agctgtttgg
     2701 cctgaataca catcagaaac gccacaatac cggttatgac ataaccgaga atttccagat
     2761 gtggtgccag tgcactaccg taaataatga ccatcgccca caccacgcca ctaagcaggg
     2821 ttgcagcgga gatcgccagc cctttcagcc caccttgcgg gcaggcaaag taggccgtac
     2881 agcccaggaa gcccgcccag cttagtaagc ccagggaaac agccacccat ccccagatac
     2941 cggagagaat gcctgttgtg attgcaatag agagaagtat gttcatgccg cgcatgatag
     3001 cagaaaacgc ggccatgaat gagatcgagc acacatttta tgcaacaaca aattatcttt
     3061 tgcagttatg agtgacgatg atcacattta ctcttcagaa tcttcttcca gctcgtccca
     3121 cattgcacta atggcatcac gggttagcgg tgccatggtg cgccagaaag gcgtggttgc
     3181 atgggcctcc actttgccga ggaacgcacc acaccagggt aacagatact cactgaacag
     3241 tgtttccagt gcttcgctct catcttccgt tgactgatct tccagccagg aagccgcaag
     3301 cagcaatgtg ccgatgtgat cggctggcgt atccgccagt ggcatccctc gttcggaaag
     3361 aaaagcgcgc acttccgctt ccgttgcgtc ctcaacccat gcgctacgat atggcggcac
     3421 ggcacattca tcgccgataa acaacgcatt gtaatcggca gagacttgcg ccatatcaca
     3481 gcttttctgt aaacgcgtca gtaactcatc ctgctccagt ggccagttcg cagccagttt
     3541 cccctcacga atcagggtaa acagcggcac cagtaaagga tcttgcggtt ggcggtaata
     3601 gagcgaaccc agcacacgac agaggataga aaactcgttc attaatatat ttccattact
     3661 agtaattaaa ggtctgcaaa ttccgcaatc ggtgccatac cgcgagattc aaggaagttc
     3721 agtaagcggc gcggagaaac attcaaaatg cgctctggcg gaaaatctac cgcgtcgagg
     3781 attttaagac actcttcaaa ttcccccatg gtaaacgcgg tgtgagaatc cgagcctaat
     3841 gccacccaac ctccagcatc acgtaccgct gcggctaccg cacgacagtt gtcttcactg
     3901 cccttacgtg agtgtaaaaa tgaagaatta ttgatttcca gcgccacctg atgtttcgcg
     3961 gctgcttcag caacggcttt cacatctatt tcatatttgg gatttccggg atggcttatt
     4021 atatgcacat tgccgctggc gatagtggcg atcatcgctt gtgtattggt cgctttgtca
     4081 tgtggtgcaa aaaccggctc atgaaaaccg gcaataatca gatccagcga gtcaaacatt
     4141 ttaccgctgc aatcaatttc accatcaaca tttttaatgt tagcttcgat gccgcgcagg
     4201 atccctaccc catcaaccac tcgcggccag atacgcatgt taatgaagtg ccagtgatgc
     4261 ggagcatctt ccatatccgg gccatgatcg gtgatcgcaa aaagtttaat gcccttttgt
     4321 ttggcctggg caatgtaatc actcaatgtg ctatatgcat gtgtgctggc aacggtatgc
     4381 atatgaaggt cgacgggata cataactctc tcctgtattc aattcccgcc aaggatagca
     4441 ggaatcctga tgctttatta gtagccgcgt gcgcggtcga cttgcccgca gaccttctcc
     4501 cctttttcga gctgggcaat agtgcgagaa atgtactcca cagcttcagc gggacgggta
     4561 atcgcggcga catgtggtgt tatcgtcacg cgtggatgtt gccagagcgg actttcaggc
     4621 ggtaagggtt cacgattaaa aacatccaag catgcgcctt taactttccc gctatccagc
     4681 gccgcgagca ggtcatcttc cacaacatga acaccacgcg ccaggttgag gagatacgcg
     4741 ccatccggta atttttcgag taattgttga ttaataatgc cgacggtttc aggggtattc
     4801 ggtaacaaat taatcaatac ccgacattgg ctcagaaatg cagacagttc ttcccgtccg
     4861 gcaaagcttt gcacgccagg ccacgattta cgggttcgac tccagcaacg cagcggaaag
     4921 cgccaggttt gcagactctg agcaacttta ctgcccagta cgcctgcgcc caaaatgccg
     4981 atggtaaaat cttcccgatg atattccggc agcggttgcc aatgcgaact attttgctgg
     5041 atgcgataat cgtcaaaacg tcgaaaccaa tgcagcacct gactgacagc atattcctgc
     5101 atttgctcgc ccataccggt atcttccagg cgaaaaagtg gaacagaagg gttcagcatt
     5161 tcagggtgtg cctgtagctt gctcaaaata gaatcaacac cggccccgag tgcgaacacc
     5221 gctttaagat cgcgccctgc cagcatttca acaggaggat gccagactaa agcataatca
     5281 gcagagtcat tatctccgct tttccacgct ctgactcttg cctgaggaat agctttgcgc
     5341 agtgcctcaa tccaccattg ggtatcgaac gttgggtgat aaaagatgat atccatactg
     5401 actcccgaaa agcgttgtgc gaaatttatt cgcacttatc gttatgatct acaaaggcca
     5461 ccagcataac aaatccgtgg tcggtggcaa aaaaagcaga tttcgcttat taaaaccaca
     5521 cattgattga agtttgaata aacgcgcgat tttttcaaaa agtttgttga cctcaggtca
     5581 tgatttccct aaattagcgc ccgttccagc aagacaggaa cgacaatttg gtgaggtgtc
     5641 cgagtggctg aaggagcacg cctggaaagt gtgtatacgg caacgtatcg ggggttcgaa
     5701 tccccccctc accgccatat ttaaagaaga gctcgtacga aagtacgggc ttttttttcg
     5761 tatattgcac acaccggggg gatgagaagc cccgaccggg gttcgacaac tggcgacagc
     5821 cagttggaca gaccgtgaac gcagtgagcg ggctgcccgc agggcgagcg aagcgagtca
     5881 atccccccct caccgccata tttaaagaag agcccgtacg aaagtacggg cttttttttc
     5941 gtatattgca cacaccgggt gcccccgccc acgtctgtta gggcgaggga aaattgtgca
     6001 catcaactgt ctggttactc agtcaacaat caaaatgcgc aagatagtag agaacatata
     6061 tgcgttatct ggtcacttcc tgtaacttcc tcacttatta aaatgccccc aacggaaata
     6121 catgtcacat gccatcataa aatcgttatg ccagaggcat cgacaatgga aaaaatagta
     6181 aattaagcac ggtacatagc agagtaaaaa ataggcttca aaccaaatcc gccattccac
     6241 aagcgttgcc aataccgaca ccagcgtaat ccacatcatt tcaataacca gcaatatgaa
     6301 taaccgaagc caaagcggct tggcaaaaaa ggtcgacaac gcattttcac tggagaaaaa
     6361 cagatcgatt tcctgcaaaa aaagcctgcc cagaaaagcg taccgcttgg agaagcgcag
     6421 aaaaaacaga ttaatcgcca acactacgcc acaaagaatg agcaatgcaa ttatcgattt
     6481 aaacgttaac cactgcccct ctgtcacaaa agaatcactg ttacgtaacg ggctgatagt
     6541 aagcaggtta acaaccgttg agatgcacac cgaaagcagc acatagcgag tcagactttt
     6601 atattttact tttttcgcta ctgaaatagc gacctgcttg acataactgg tgcggatcat
     6661 ccgaaacagc agaaagatga ccaacggccc aagtaacgaa atcaggaaca gcgcatataa
     6721 cgagggtaga agctggtata gcccccaact cattgcactc aacatgatta acgacagaaa
     6781 tcctgccacc agaggttgtg taagaaataa ttctattttg tggttctgct ttttcttaaa
     6841 ccacgaaaaa tcgatattct tcgtgacgca tttataagcc cagtacgcct tgagatcaaa
     6901 aataaaatta tacagcacca gggccagaat cccactaacg acaccaatat actctgatgg
     6961 aaaagtaaat cctgcatttt tccaataaaa gcctatcaga ataagtccaa tagcataaat
     7021 gtatatcaga tttcgtttat cctgaaagcg aataaaagtt aaataatcag gaatcatttg
     7081 aatacctttg catcactaaa tgctttacga caatgcataa ctcgttgctg atccacgcca
     7141 gcaaaatcat ttaacatatt ccccaaatag ggcttattac ttatttcaag gaaagctacc
     7201 ggggttggca tattcagaaa tgtatcgaga gattcagtcc atttcaccgt atgggttaag
     7261 tgtaatgaga gattttcttt gataattgac ggagcgacct cgctttttgc tgttacgttc
     7321 atcagaacct gatgctctgg cgaagcgata tccagtccgg caagataatc gcgcattgcc
     7381 tgaacgccgt cttccatcaa acgcgtgtgc caggcaccgc tcacaccgag tttaaccggt
     7441 tcgtatccag cggccatcag cagcgtggca aattcattca acgaggcctg cgttccccca
     7501 ataacctgct ggcgcggcgt gttatcacag ctaatatcca gcgcaatgcc tgattccgta
     7561 atcatcgtct gcagttgttc gcggttgatg cctttaaccg
//
LOCUS       DBANUU010000180         7574 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000180 DBANUU010000000
VERSION     DBANUU010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.183"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(187..263)
                     /locus_tag="ACIE68_004200"
     tRNA            complement(187..263)
                     /locus_tag="ACIE68_004200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Arg,seq:acg)
     gene            complement(267..359)
                     /locus_tag="ACIE68_004201"
     tRNA            complement(267..359)
                     /locus_tag="ACIE68_004201"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(323..325),aa:Ser,seq:gct)
     gene            complement(675..860)
                     /gene="csrA"
                     /locus_tag="ACIE68_004202"
     CDS             complement(675..860)
                     /gene="csrA"
                     /locus_tag="ACIE68_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFX5910422.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1095..3725)
                     /gene="alaS"
                     /locus_tag="ACIE68_004203"
     CDS             complement(1095..3725)
                     /gene="alaS"
                     /locus_tag="ACIE68_004203"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFX5910423.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3853..4353)
                     /gene="recX"
                     /locus_tag="ACIE68_004204"
     CDS             complement(3853..4353)
                     /gene="recX"
                     /locus_tag="ACIE68_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFX5910424.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLVAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4596..5657)
                     /gene="recA"
                     /locus_tag="ACIE68_004205"
     CDS             complement(4596..5657)
                     /gene="recA"
                     /locus_tag="ACIE68_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFX5910425.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5737..6234)
                     /gene="pncC"
                     /locus_tag="ACIE68_004206"
     CDS             complement(5737..6234)
                     /gene="pncC"
                     /locus_tag="ACIE68_004206"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFX5910426.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6379..7464)
                     /gene="mltB"
                     /locus_tag="ACIE68_004207"
     CDS             complement(6379..7464)
                     /gene="mltB"
                     /locus_tag="ACIE68_004207"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFX5910427.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
BASE COUNT         1764 a         2129 c         1827 g         1854 t
ORIGIN      
        1 tactttactg cagatttttt gataccgcta ctaaagccgt atcaagtaag agatggtgca
       61 tccgggagga ttactcgcct tgcggctcgc ccttcgggcc gttgctaaag caacgttatc
      121 ctccctggtg cctgcggtta taccgccacc tcttccatac cttcatcgct gaaagtacgt
      181 agaatatggt gcatccggga ggattcgaac ctccgaccgc tcggttcgta gccgagtact
      241 ctatccagct gagctacgga tgcaaatggc ggtgaggcgg ggattcgaac cccggatgca
      301 gcttttgacc gcatactccc ttagcagggg agcgccttca gcctctcggc cacctcacca
      361 cacgcctctt acgagtgctt cgaagaactt gtttatcgct catcgtcgct gcgtggcgca
      421 catattactt tctgagactt ataagtcaaa caatttttcc tacactttta tcgtttgcac
      481 acttcacgtt caattagtct gtaaaaacgt caaaaagagt gttttatcaa cagaaaaatg
      541 gaggtctgac agatagtagt aatgcaaaaa aatggagact taagttgaat gaacgggagt
      601 aaagcgaaaa gactatagag tgaaggagaa attttgaggg tgcgtctcac cgataaagat
      661 gagacgcgga aagattagta actggactgc tgggattttt cagcctggat acgctggtag
      721 atctcttcac ggtgaacaga aacttccttc ggggcattta cgccaatacg tacctggttg
      781 ccctttaccc ctaaaactgt cacggtgacc tcatccccaa tcatgagggt ctcaccaact
      841 cgacgagtca gaatcagcat tctttgctcc ttgaaagatt aaaagagtcg ggtctctctg
      901 tatcccggca ttatccatca tataacgcca aaaagtaagc aatgacaaac acattacatg
      961 taagcagtca tggcattaca ttctgttaaa cctaagttta gccgatatac acaacttcaa
     1021 cctgacttta tcgttgtcga tagcgttaat gcgaatgccg tgaagcgagt ccacggcatt
     1081 gcctgacgct tatattattg caatttcgcg ctgacccagc ctttcacact ggctaacgct
     1141 gcaggtaagg ccgcagcatc cgtaccaccg gcttgcgcca tgtcaggacg tccaccaccc
     1201 ttgccgccca cctgctgagc gaccatacca atcagttccc ctgctttcac acgatctgtg
     1261 acgtccttag atacgcctgc aatcagagaa accttacctt cggctaccgt tgccagcacg
     1321 ataattgtcg accccagctg attttttaaa tcgtcaacca tggtacgcaa cattttcggc
     1381 tcaacaccgc taagctcgct aaccaacagc ttaacaccat taacatcaat tgccttactg
     1441 gaaagatttg cgctctcctg tgcggcagct tgttctttaa gctgttgtag ttctttttct
     1501 aactgacgcg tacgttccag tactgagcgc actttatccg ccagattatt gctatcgcct
     1561 ttcagcagat gcgcgacttc gcttaagcga tcgctgtctg catgaacggt ggtgatagcg
     1621 ccttctccgg ttaccgcttc gatacgacga acgcctgcag cagtacccga ttcagagatg
     1681 atgcggaaca gaccaatatc accagtgcgg ctggcgtgag taccgccaca cagctcggtg
     1741 gagaaatcgc ccatgctcag cacgcgtaca cgctcatcat acttctcgcc gaacagcgcc
     1801 atcgcacctt tcgctttcgc cgcttcgaga tccatgatgt tggtttcgat cggcaaattg
     1861 cgacgaatct gtgcgttcac caggtcttcg accgcacgaa tctcttccgg tttcatcgct
     1921 tcgttgtgtg agaagtcgaa gcgcagcacc ttgtcgttaa ccagtgaacc tttctgcgat
     1981 acatgagtcc ccagaacctg gcgcagcgca gcgtgcatca ggtgcgttgc ggagtgattc
     2041 agacgaatac gggcgcgacg agcctcatca acatcagcct gcacagcgtc gcccactttc
     2101 agagaacccg cagcaagttt accgatgtga ccaatcgcct ggccgtattt ctgcgtatct
     2161 tccaccgcga aggagaagtt agcgcctttc agttcgcctt tatcaccaac ctgaccgccg
     2221 gattccgcat agaatggtgt ttgatccagc acgaccacag cttcctggcc tgcattgatg
     2281 gcatcaaccg ctttaccatc aacaaacagc gcggtcactt tgccgttcag ttccagatgg
     2341 tcatagcctt taaattcaga tgcactatca acacggatca ttgcgttgta atcggcacca
     2401 aagccgctgg cttcgcgcgc gcgacgacgc tgctcttcca ttgctgcttc aaaaccagct
     2461 tcgtcaactt tgatgttgcg ctcacgacaa acatcagccg tcaggtcaac cgggaagcca
     2521 taggtgtcgt acagacggaa agccgtttca ccatccagcg tatcaccaga aagttttgcc
     2581 agctcttcat ccagcaacgc cagaccgcgc tccagagtac gggcaaactg ctcttcttca
     2641 gtcttcagca cctgctcaac ctgcgcctgc tggcgtttca ggtcttcacc cgcagagccc
     2701 ataacgtcga tcagcggacc aaccagtttg tagaagaagg tttctttcgc gccgagcata
     2761 ttgccgtgac gcactgcgcg acgaatgata cgacgcagta cataaccacg gttttcattg
     2821 gacggcatta cgccatccgc gatcaggaac gcacaagaac gaatgtggtc agcgattacg
     2881 cgcagcgatt tattgctcag atcggtcgcg ccagtgactt tcgctaccgc ctggatcaac
     2941 gtgcggaaca ggtcgatgtc atagttagag ttaacgtgtt gcagcaccgc agcaatacgc
     3001 tccagaccca taccggtatc tacagacggc ttcggcagcg gttccatcgt gccatctgcc
     3061 tggcggttga actgcatgaa gacgatgttc cagatctcaa tgtagcggtc gccgtcttct
     3121 tccggacttc ccggagggcc accccaaatg tggtcgccgt gatcgtagaa gatttcggtg
     3181 cacgggccgc acggaccagt gtcacccatc tgccagaagt tgtcagatgc gtatggcgca
     3241 cctttgttat cgccgatgcg aataatacgt tcgcgcggga tcccgacttc tttttcccag
     3301 atttcgtagg cttcgtcgtc gctttcatag acggtaaccc acagacgctc tttcggcagg
     3361 gcaaaccatt tttcgctggt cagcagttcc catgcaaact gaatggcatc gtgtttgaaa
     3421 tagtcgccga agctgaagtt gcccagcatt tcgaagaagg tatggtgacg cgcggtgtaa
     3481 ccgacgtttt ccaggtcgtt gtgtttacca cccgcacgca cgcagcgttg ggaagtggta
     3541 gcgcgggaat aattacgctt gtcgagccca aggaacacat ccttgaactg gttcatcccg
     3601 gcgttggtaa acaacaaagt tgggtcgtta tggggtacca gggagctgct ggcaactacc
     3661 tgatgtccct tactatggaa aaagtcgaga aacgcctgac ggatctcagc ggtgctcttg
     3721 ctcataatta tcctgaaatc aagctaacga aatatcgcca ccagctccag cgtgtcttaa
     3781 ccgccgggct ggtaactgaa aagtgggaat aagataagtt ttcttgactg ggaagtaaaa
     3841 taccgtatgc gttcagtcgg caaaatttcg ccaaatatcc tggatatctt ccatcagata
     3901 gccacgatag agcagaaaac gctggatctt aactttttct gaaaagacag ttggcaaagg
     3961 ttcaccatat tttcgcgtcg cctgatcgcg cgccagtgcg caccagtcga tgtcacattc
     4021 acgcatcgct ttttctgtcg cttcgcggga aatacctttc tgattcagtt cctgacgaat
     4081 acgtgcaggt ccataacctt tgcggctacg gctggcgata aagcgcgcaa caaatcggct
     4141 gtcatcgaga tagccatgtt catggcacca ggcaataacg cgctcgtaat cttctgccgt
     4201 agcatcaatc tcttctgggc catttttgcc cataatcggt gccacgagtt tacgtcgcag
     4261 ttcttgctca ctgtgatcgc gcaccgccag aatgcgtacc gcacgatcca acaggcgagc
     4321 atatgccggg cgacgggacg ttgattctgt catggcatat ccttacaact taaaaaagca
     4381 aaagggccgc agatgcgacc caggttttgc tgataaacag atttaatagt caatgagtgt
     4441 gcagaatgtt cccgcaggcc caattcgtta gaggatcgag gtgagagtaa tttttcacgc
     4501 cagtgctggc ctgttcccct caccctaacc ctctccccaa aggggcgagg ggaccgtccg
     4561 tgcacacatt atctattatc atcaataacg aacgattaaa aatcttcgtt agtttctgct
     4621 acgccttcgc tgtcatctac agagaaatcc ggcgttgagt tcgggttgct cagcagcaac
     4681 tcacgtactt tcttctcgat ctctttcgcg gtttccgggt tatctttcag ccaggcagtt
     4741 gcattcgctt taccctgacc gatcttctca cctttgtagc tgtaccacgc gcctgctttc
     4801 tcgatcagct tctcttttac gcccaggtca accagttcgc cgtagaagtt gataccttcg
     4861 ccgtagagga tctgaaattc agcctgttta aacggtgcag cgattttgtt cttcaccact
     4921 ttcacgcggg tttcgctacc caccacgttt tcgccctctt tcaccgcgcc gatacgacgg
     4981 atgtcgagac gaacagaggc gtagaatttc agcgcgttac caccggtagt ggtttccggg
     5041 ttaccgaaca tcacaccaat tttcatacgg atctggttga tgaagatcag cagcgtgttg
     5101 gactgcttca ggttacccgc cagcttacgc atcgcctggc tcatcatacg tgccgcaagg
     5161 cccatgtgag agtcgccgat ttcgccttcg atttccgctt tcggcgtcag tgccgccacg
     5221 gagtcaacga cgataacgtc tactgcgcca gaacgcgcca gggcgtcaca gatttccagt
     5281 gcctgctcgc cggtgtccgg ctgggagcac agcaggttat cgatatcgac gcccagttta
     5341 cgtgcgtaga ttgggtccag cgcgtgttca gcatcgataa acgcacaggt tttaccttca
     5401 cgctgcgctg cggcgatcac ctgcagcgtc agcgtggttt taccggaaga ttccggtccg
     5461 tagatttcga cgatacggcc catcggcaga ccacctgccc caagcgcgat atccagtgaa
     5521 agcgaaccgg tagagatggt ttccacatcc atggaacggt cttcacccag gcgcatgatg
     5581 gagcctttac caaattgttt ctcaatctgg cccagtgctg ccgccaacgc tttctgtttg
     5641 ttttcgtcga tagccatttt tactcctgtc atgccgggta ataccggata gtcaatatgt
     5701 tctgttgaag caattatact gtatgctcat acagtatcaa gtgttttgta gaaattgttg
     5761 ccacaaggtc tgcaatgcat acgcagtagc ctgacgacgc accgcatcac ggtcaccgct
     5821 gaagcattcc cgccgggtaa tgccttcacc gcgggcagtg gcaaaagcaa accagacggt
     5881 gccgacaggc ttctcttcac tgccgccatc cggcccggcg ataccactaa tagacacggc
     5941 ataatcagca cgagccgctt tcagtgcacc tatcgccatt tccaccacga cgggttcact
     6001 caccgcgcca tgctgcgcca gcgtctcttc gcgtacgccg atcatctgcg ctttggcttc
     6061 gttactgtag gtgacaaatc cgcgttcaaa ccaggcggag ctaccggcaa tatcggtaat
     6121 cactttcgct acccaaccac cggtacaaga ctcggcagtt gttacggttg cgccacgggc
     6181 tttcagcgcc tgcccaacct gttcacttaa ctgcatcagt tcactgtcag tcatcacaac
     6241 ctctgtcagt caaaaattta tgccggacaa gatagcactt tcgtcaggaa gatggtgttg
     6301 gcgttgcaat tttacgagga ggattcagaa aaaagctgat tagccagagg gaagctcacg
     6361 cccccctctt gcaaatagtt actgcacgcg cgccagcgcc acggcttgtc ctaactgcca
     6421 gaccgccatt gcgtaatggg tgctgtggtt ataacgggta atggtgtaga agttcggcag
     6481 accgtaccag tactggtaac cggtaccgac atccagacgc agcaggctgg cttgttgatg
     6541 gttgcccagc ggctgctgtg gcgttaaacc tgccgtggca agctgcgaaa tgctgtacct
     6601 ggttttgaag ccatttggca agcctggagc ctgaccgttt gccattaccg cgacctgatc
     6661 gcctttcacc cagccgtgcg ctttgaaata gttcgccacg ctaccgatcg catcaaccgg
     6721 atcccacagg ttgatatgcc cgtcgccgct gaaatctacc gcatattgtt tgtaagacga
     6781 cggcataaac tgtccgtagc ccatcgcccc ggcaaaggaa cctttcagat tgagcggatc
     6841 gtcctgctcg tcgcgcgcca tcaacaggaa ggtttccagc tcgccagaga aatactccgc
     6901 gcggcgtggg tagttaaatg acagcgttgc cagcgcatcg aggatgcgag ttttccccat
     6961 cacgcgcccc cagcgggttt caacgccgat aatcccgacg ataatttccg gcggtacgcc
     7021 atacacctgc cacgcgcgat tcaacgcatc ttcatactga ttccagaaaa ccacaccgtt
     7081 ctgcacgttg tccggcgtaa taaatttttt gcgataacgg agccatgcgc cgttcggacc
     7141 tgatggcggt ttcaccgatg tggttggtgc ctggttatcc atcagccgca gtaccgaatc
     7201 cagacgcttc gcctgggaga gaatttcctg caactgctga cgatcgaaac cgtgtttgtt
     7261 caccattttg tcgatgaact gctgggcatt cgggttatta gcgaaatcgc cgcccatctg
     7321 catcacattg tgctgcggct caagcaggaa gccgccagac ggcgttccgg tggtcgtttc
     7381 agtctcagta ggttttggct tgctgctaca ggcggcaagc aacacaaaaa ggggaagcaa
     7441 tgttacataa cgacgcttga acatgagggg tccatttaac agattcaacc aggggcaagt
     7501 atggtaaagc atcacgcccc acacaaggaa gcggtagtca ctgcccgata cggactttac
     7561 ataactcaac tcat
//
LOCUS       DBANUU010000181         7510 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000181 DBANUU010000000
VERSION     DBANUU010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.184"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..293
                     /locus_tag="ACIE68_004208"
     CDS             <1..293
                     /locus_tag="ACIE68_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910428.1"
                     /translation="IDLVNKIRGKKDFMYICTLCCFAEEVKGDYSHYRTFLFDIGNQY
                     KVKLTQSGKKELSLTLEFNDTIIESQKVTGNKAKHILEDIEKFYRNKPDTYY"
     gene            complement(515..1708)
                     /gene="tyrB"
                     /locus_tag="ACIE68_004209"
     CDS             complement(515..1708)
                     /gene="tyrB"
                     /locus_tag="ACIE68_004209"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFX5910429.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLKQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1961..3040)
                     /gene="alr"
                     /locus_tag="ACIE68_004210"
     CDS             complement(1961..3040)
                     /gene="alr"
                     /locus_tag="ACIE68_004210"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFX5910430.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(3093..4508)
                     /gene="dnaB"
                     /locus_tag="ACIE68_004211"
     CDS             complement(3093..4508)
                     /gene="dnaB"
                     /locus_tag="ACIE68_004211"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFX5910431.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4591..5574
                     /locus_tag="ACIE68_004212"
     CDS             4591..5574
                     /locus_tag="ACIE68_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFX5910432.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5740..5982)
                     /gene="pspG"
                     /locus_tag="ACIE68_004213"
     CDS             complement(5740..5982)
                     /gene="pspG"
                     /locus_tag="ACIE68_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFX5910433.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(6116..7153)
                     /gene="dusA"
                     /locus_tag="ACIE68_004214"
     CDS             complement(6116..7153)
                     /gene="dusA"
                     /locus_tag="ACIE68_004214"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFX5910434.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
BASE COUNT         1944 a         1945 c         1824 g         1797 t
ORIGIN      
        1 ttattgattt agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt
       61 gctgttttgc cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata
      121 ttggtaatca atataaggtt aaacttacac aaagcggaaa aaaagagctc tctttaactt
      181 tagaatttaa cgatactata attgaatctc agaaagtcac aggcaataag gcaaagcata
      241 ttcttgagga tatagaaaaa ttctatcgta acaaacccga tacttattat taaatttaat
      301 aatagttaga tgtgcagaaa ttattactcc acccaaaaaa acaggataag gaattccaga
      361 taattaggat aagcttaaca ataaaagaac taataacgtt tattatctct cacgtagaac
      421 gatggcatca aaaaaatcat ctataaagaa agagtgaaag cgacgacagt ttaatttcac
      481 tgcaggctgg gtagctccag cctgctttcc tgcattacat caccgcagca aacgcctttg
      541 ccacacgttg tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga
      601 gatagacacc aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac
      661 tgaacatgcc gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta
      721 ataccttcac caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg
      781 ccagccagct ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa
      841 aattcggcgg gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc
      901 cagcggcttc ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg
      961 agaaaatttt cgagaacgaa ttgctcacca gagcgggtaa tccagcgctg gcaatggcgc
     1021 gaatagcgta ggcatcctct tccataccgg caccaaatcc ttgataggca atatcgagga
     1081 atggaataag ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga
     1141 gatcggcacc cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta
     1201 atgttttcag cgtcgccaac aggtcattaa agcgcacgcc gttagtcgct tcgtcatacc
     1261 aggggtaagt actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccagg
     1321 taggatcgct gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca
     1381 ctttcaatgc cccggagccg ccaagggttt gaatggttgc tacgcgctgt tgtttcagta
     1441 ccggatggtc cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt
     1501 ccatcggtaa ataaagcgaa gcgccatgag gctgcgcatt caggcgcgct tccgcctccg
     1561 ccacggcttt cagttgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat
     1621 tcactttgtc gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc
     1681 cagcgtaggc gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt
     1741 taaaacacaa taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa
     1801 ttagcaacag agtgagactc atgacaaacg tacatccgcc agaggcacga cctttataag
     1861 agcatgaaag aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc
     1921 acgccgcatc cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg
     1981 cgacccttga agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga
     2041 tacgttctac gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg
     2101 cttgtggacc taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt
     2161 cgcgaccgtt caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat
     2221 agcccatcgc gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac
     2281 caacaggttc tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca
     2341 ctggctgaca gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa
     2401 gaatgatgcc cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg
     2461 atgcggcaat ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt
     2521 gtttctcggt tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca
     2581 ccggctgacg aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg
     2641 gccttacgcc cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt
     2701 ccaggctagc ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat
     2761 gttgcgcaga aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag
     2821 gtttggtgat tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg
     2881 cgtcagcatc ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt
     2941 tcaccaccgc aaccatttta ctggcagggg ccagttcacg aagacgttgc aggttgtgtc
     3001 gcagagcgcg gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataagtgt
     3061 ttgctttaat tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca
     3121 tagttgtcga agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg
     3181 ttacgttgtt taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac
     3241 acctcatcac gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca
     3301 cgcaggtcgg agttgaccgg gcgtttgtcg gcacgttgtt ccagagaacg gttcaactgg
     3361 gacagcgcca ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt
     3421 tctgcaattt ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag
     3481 tcgatcatga taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc
     3541 acttccgttg gcgtcaggcc ggaggagtca tcgatataga tattgcgttt ttcgagcaaa
     3601 atacccatgg tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt
     3661 ttagtctggt caacgcgcga cagcgacgcc agagaacgca tcataatctg ttctgaaggc
     3721 atctccagcg agaagataag tactggttta tcctgcaaca tcgccgcgtt ttcgacgagg
     3781 ttcatcgcaa atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac
     3841 ggctgcaagc cagcggtttt tttgttgaga tcgtcataac cggtgtttac tccggtaacg
     3901 ccatcgtgtg gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg
     3961 gcgatgttct tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg
     4021 gattcagcaa ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca
     4081 gcaatctcat tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtca
     4141 gcataagcac tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca
     4201 aaaccaccga cgctatcgag ttgcccctgg cgttccagcg attccgcaag ggtaatcaga
     4261 tcgataggac taccgctttc ctgcaaacgc gccatttcag taaagatatg gcggtgtggg
     4321 cgggtgtaaa aatcgtctgc caccacacgc tcggctacat cgtcccagcg ttcgttatcc
     4381 agcattaaac cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc
     4441 ccggcaactt gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt
     4501 cctgccatag tgaatggagt taccgagata aagaatgggt cgaaacttta ccatatgaag
     4561 cagaccctga cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac
     4621 ggtggcccgg aagtacttca agccgtagag ttcactcctg ccgatccggc agagaatgaa
     4681 atccaggtcg aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc
     4741 ctttacccgc cgccatcact acccagcgga ttaggtaccg aagcggcagg catcgtgagt
     4801 aaagtcggca gtggtgtaaa gcatattaag gcaggcgatc gtgtagtcta tgcgcagtcg
     4861 gcgttaggcg cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct
     4921 gcggcaattt cttttgagca agctgcagca tctttcctga aaggcttaac ggtttattat
     4981 ctgctgcgca aaacctatga aattaaaccc gatgagcagt tcctgttcca cgcagcagct
     5041 ggcggcgttg gcttgattgc ctgccagtgg gcaaaagccc tgggcgcgaa acttatcggc
     5101 accgtaggaa ccgcgcaaaa agcgcagagc gcgctaaaag cgggcgcgtg gcaggttatt
     5161 aactatcgtg aagaggatct ggtcgagcgg ttaaaagaga tcaccggcgg taagaaagtg
     5221 cgcgtggtgt acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa
     5281 cgccgcggct taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta
     5341 ggcattctca atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc
     5401 accacgcggg aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt
     5461 gtgattaagg tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg
     5521 catgagattc tggaaagccg ggcgacgcaa ggttccagcc tgttgattcc ataaaagaaa
     5581 tagggcttcc acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca
     5641 cgatgaatct gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct
     5701 aaggataaaa ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata
     5761 acgctgatat ttcggcactt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat
     5821 cgccagtagt aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat
     5881 aatggccgtc gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat
     5941 cagcatgaca aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg
     6001 aaatctcttg ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa
     6061 acgccccgcg acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac
     6121 gcttatccgc caccagtttg agcgcgtgtt ccagcacatt aatgtctgca cccgctttat
     6181 gggcattttc acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaagc
     6241 ccaacatatg ccgggtaata tggccgagat acgtcccctg gctgagttca cgctcaatgt
     6301 acggatacat ggcgcgcact accgccaccg gatcggcatc ggtatccgag gaaccaaaga
     6361 tctcccggtc tactgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca
     6421 cgccatccat atgttgcagg tgtgctttgg cctcttccag cgacttgata ccaccgttaa
     6481 tcgacattgt cagatgcgga aagtcacgct tcagttgata cacacgcgga taatcgagcg
     6541 gcgggatttc acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga
     6601 tgataaacat ctcacactcg cctttgccgg aaacagtgtt gatgaaatcg cagagaaatt
     6661 catagctgtc ctggtcatcg atgccaatac gcgttttcac cgtcactgga atcgacacca
     6721 catcgcgcat cgctttcaca cagtcggcaa ccagctgcgc attacccatc agacacgcac
     6781 caaacatgcc gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat
     6841 atccacgcgc ttctgccagc ttcgcacact gtgccagcgc cgccggatcg ctaccgccga
     6901 gttgcaacgc taccggatgt tcttcttcac tgtacgccag gtaatcacct ttaccgtgaa
     6961 taatcgcccc tgtggtcacc atttcggtat acagcaacgt attgcgggaa agcagacgca
     7021 agaaatagcg gcaatgtctg tccgtccagt cgagcattgg tgcaacgcta aaccgaccac
     7081 tccagtgaac gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag
     7141 aattaccgtg cattttcaaa tgtagagatt attttatcga tatatcattg ggttatgtta
     7201 ttcagcatca ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg
     7261 cctgtctcat tgcaaaagaa aattgagatc aaatcaaggc atgaagctct cacgaagtga
     7321 tggaaataat cttattagcc gttagccttg ttaaggacaa tgataaacaa tccaggttcg
     7381 acgataaata aaaaatcaca cattaaactc cggtgatatc tcttcctgct aatggcactg
     7441 atagaagaaa aaagaaccca ataagtatta ggttctttta tgtaatgcct tccataccat
     7501 cgaagaactt
//
LOCUS       DBANUU010000182         7505 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000182 DBANUU010000000
VERSION     DBANUU010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.185"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..372
                     /locus_tag="ACIE68_004215"
     CDS             <1..372
                     /locus_tag="ACIE68_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910435.1"
                     /translation="FDKKNMKPLLLANNSNEYPGLFSAIQHKQQNVVETVYLALSDHA
                     RLFGFTAEDIMDFWQHKAPQKYSAFELAFEFGHRVIAELILNTLNKMAESFGFTDNPR
                     YIAEKNYMEALLKKASPHTVR"
     gene            complement(341..2077)
                     /gene="aceK"
                     /locus_tag="ACIE68_004216"
     CDS             complement(341..2077)
                     /gene="aceK"
                     /locus_tag="ACIE68_004216"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFX5910436.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(2260..3564)
                     /gene="aceA"
                     /locus_tag="ACIE68_004217"
     CDS             complement(2260..3564)
                     /gene="aceA"
                     /locus_tag="ACIE68_004217"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFX5910437.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(3594..5195)
                     /gene="aceB"
                     /locus_tag="ACIE68_004218"
     CDS             complement(3594..5195)
                     /gene="aceB"
                     /locus_tag="ACIE68_004218"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFX5910438.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(5464..6393)
                     /gene="metA"
                     /locus_tag="ACIE68_004219"
     CDS             complement(5464..6393)
                     /gene="metA"
                     /locus_tag="ACIE68_004219"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFX5910439.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            6550..6993
                     /locus_tag="ACIE68_004220"
     CDS             6550..6993
                     /locus_tag="ACIE68_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFX5910440.1"
                     /translation="MVISIRRSRHEEGEGLVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGA"
     gene            complement(7056..7439)
                     /locus_tag="ACIE68_004221"
     CDS             complement(7056..7439)
                     /locus_tag="ACIE68_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaA family stress response protein"
                     /protein_id="HFX5910441.1"
                     /translation="MSVLYIQISRNKITVRDLESKREVSGDAAFSNQRLLIANFFVAE
                     KVLHDLVLQLHPRSTWHSFLPAKRMDIVVSALEMNEGGLSQVEERILHEVVAGATLMK
                     YRQFHIHAQSVVLSDSAVMAMLKQK"
BASE COUNT         1836 a         1904 c         1907 g         1858 t
ORIGIN      
        1 ttcgataaaa aaaatatgaa gcccctcctc ctggcaaata attctaatga atatcccggt
       61 ttgttttcag cgatacagca taaacaacaa aatgttgtag agacggttta tcttgcttta
      121 tctgaccatg cacgcctgtt tggatttacc gctgaagata ttatggattt ttggcaacac
      181 aaagcgccac aaaaatactc tgcctttgag ttggcttttg aatttggtca ccgggttatt
      241 gctgaattaa tccttaatac attaaataag atggctgaaa gctttggctt tacggataac
      301 cctcgataca ttgcggagaa aaattatatg gaagctttac tcaaaaaagc atctccccat
      361 accgtacgct aaatctttgc ctgcgccgat acgcataaac atcttccaca tgcccttcac
      421 gtatgcggtt ttgtagtgcg cgccagtaat cagcgcggaa caggtcggcg tgcatctctt
      481 caaacagcgg accaatacgc gggtcggcgc atagccagtg gcgaaactct tccgggaaaa
      541 catcgcccgg cgagacgctg taccacggtt cgctggcaag ttcgtcttcc ggatagcgcg
      601 gcggcgggat gtcgcggaaa ttcacttccg tcatgtagca aatttcatcg taatcataaa
      661 aaaccacacg cccgtgacgg gtgacaccga agtttttaaa cagcatgtcg ccaggaaaaa
      721 tgttagcagc ggcaagctgg cgaatagcgt taccgtattc ttcaatggcg tcgcgcaact
      781 gctgaccttc cacttgttcc agccagatat tgagcggcac catccgccgc tcgatataga
      841 gatggcgaat tacaatctgc tcaccaagat cggtgatttt ttccgctgct tcctggagca
      901 gtaattccat taatgccggg gaaatatgcc gcttctccag cacaaagttt tcaaactcct
      961 gggtgtccgc cattcggccc acgcgatcgt gctctttcac cagttgatag caggcacgaa
     1021 cgtgagcggc agacatctct ttctgcggcg cgaacttgtc tttgatgact ttgaataccc
     1081 gatcaaagcc cggcagcgta aacaccaaca tcaccattcc acgaataccc ggcgcttcaa
     1141 tgaactgctc attacagccc tgtagataaa cgagatattc gcggtagctt tcggttttgg
     1201 cgtgcttctg gcagccgata gccatataca attcagcagt ggttttacct ggcagaattt
     1261 cccgtagcca ctcgaccagc gctgcgggca gcggtgcata aaccataaaa taagaacgcg
     1321 caaagccaaa aacaatgctc gcttcggcgg tcgtcgtcag gcaggtatca ataaataact
     1381 cgccgtcgtc cgtctggtgg atcggcagta agaagggcaa cgtgccggaa ggcgtgatca
     1441 gtttgcccac cagccaggcg gctttattgc ggtaaaacag ttcgttcgcc acctgtaaat
     1501 gactttccgc gaggttgtct gtccccagcg tttccgtcag atggcgaatg atgtaatgaa
     1561 tgtcacggct tttattctgc cagcgcaggc gcagcggcag gtcgctgata acgcgcatca
     1621 gtagagattc ccagccgtga tcggggtgaa agtctttcgc cagcgggcgg ggaatggtac
     1681 gaaagcggcg ctctggctga gagctaaaga taaaaagccg ctcgggagta agcgagcggt
     1741 ggtcaaataa ccgacagtac acggagttaa aaaagctctc cgcaatctcg aagcgcgggt
     1801 aatccggcaa cagccgcgtg taatgctctt taacgcgcag taaaaatgcc gcgtccgtac
     1861 tttggccgtt agtaatgcag cgcagttgct ccacgaccag accaacgtga tgatcgtaaa
     1921 gatggatacg gtttttcatc gcctgctgga cagcatgcca gtcggcctgt tcgaaacgct
     1981 gctgcgcccc ggaagtcact tcgaggaatc gaccatactg agcgtcgaag ccttgcaaaa
     2041 tggtttgagc aatcagtaat tccaggccac gcggcattta gcgccctcat caggagcaga
     2101 gaattgccgg atgcggcgta aacgccttat ccagcctacg ttcggcaacg gttgtaggca
     2161 tgataagacg cgcaagcgtc gcatcaggca tcgtgcaccg attgccggat gcggcgtgaa
     2221 cgccttatcc ggcctacagt cagcaacggt tgttgttgct tagaactgcg attcttcagt
     2281 ggagccggtc agcgcggtga ctgaagacgt gccgccctga ataatagtcg tcactttatc
     2341 gaagtaacct gtacccactt cctgctggtg agatacgaag gtatagccat ctttcgcggc
     2401 ggcaaattcc ggctgctgca ctttctcaac gtagtgcttc ataccctcgc cctgggcata
     2461 ggcgtttgcc aggtcaaaca tgttgaacca catgctgtgg atacctgcca gggtgatgaa
     2521 ctggaacttg tagcccatat ccgacagctg ctgctggaag ctggcaatag ttttgtcgtc
     2581 gaggtttttc tgccagttga acgacggcga gcagttataa gccagcagtt tgcccggata
     2641 tttcgcgtgg atagcttgtg caaagcgacg cgccagttcc agatccggcg tggaggtttc
     2701 acaccagacc aggtcagcat atggcgcata cgccaggcca cggctgatcg cttgctcaat
     2761 gcccgcatga gtacggaaga agccttcact ggtacgctcg ccagtaataa attcactgtc
     2821 atacgggtcg caatcggagg taatcagatc cgccgcatcg gcatcggtac gagcaaccag
     2881 cagggttgga acgcccgtca cgtcagctgc aagacgcgcc gcgaccagtt tctgaatagc
     2941 ttcctgagtt ggcaccaaaa ctttgccgcc catgtgaccg catttcttca ctgacgccag
     3001 ctgatcttcg aagtgaactg ccgctgcacc ggcttcaatc atcgctttca tcagttcaaa
     3061 ggcattcagg acaccgccaa aaccggcttc cgcatcggca acgatcggca ggaagtaatc
     3121 gacatagcgt ggatcgcccg gctcaatgcc tgcggaccat tggatctgat cggcacgacg
     3181 gaaggtgttg ttgatccgct ccaccacagc tggcaccgag tttgccggat agagcgactg
     3241 atccggatac atgctggccg ccaggttagc gtccgccgct acctgccatc ccgacagata
     3301 gactgcttca atacccgctt tcgcctgttg cagcgcctga ccgccagtca gtgcaccgag
     3361 gctgttgatg tagccttttt tcgactcgcc atgcagcaga cgccacattt tcgccgcgcc
     3421 cagttgcgcc agggtgcatt caggattgac tgaaccgcgt aatttcacca catcttccgc
     3481 gctgtatggg cgagtaatgc cttcccaacg cggttgagtc cactctttct gtaattcttc
     3541 aatttgttgt gtacgggttt tcatgtgcag atgctccata gttatgtggt ggtttacgct
     3601 aacaggcggt agcctggcag ggtcaggaaa tcaattaact catcggaagt ggtgatctgt
     3661 tccatcaagc gtgcggcatc gtcaaaacgc ccctgggaga aacgttcttc gcccagttcg
     3721 ctggcaatga ctttcatctc ttcacccagc atctggcgga acaaggcttt ggtcaccggt
     3781 ttgccattgc tcaacgtttt ttgatgatgg atccactgcc agattgaggt acgggaaatt
     3841 tcagccgtcg ccgcatcttc catcaggcca taaatcggca cgcagccgtt gccggagatc
     3901 catgcttcga tgtactgcac tgccacgcga atgttggcgc gcataccttc ttcggtgcgt
     3961 tcaccatcac aaggtgccag cagctgatcg gcagtaatcg gcgcgtcttg ttcgcgcatc
     4021 acttcaagct gatttttacg ggagccgaga atgtcgttga ataccgccat tgccgtatcc
     4081 gcaaggcctg ggtgagcaat ccatgtgcca tcgtgaccgt tattggcttc cagcgcttta
     4141 tccgctttta ctttgttgag cacctggtta ttgcgctctt catctttgct cggaataaac
     4201 gctgccatgc cgcccatcgc aaaagcaccg cgtttatggc aggttttaat caacaggcgt
     4261 gagtaagcat tcaggaatgg tttatccatc gtcactgcct gtctgtctgg caggacgcga
     4321 tcgggatagt ttttcaacgt tttgatatag ctgaagatgt aatcccaacg accgcagttc
     4381 agaccaacaa tatggtcacg cagcgcgtga aggatttcat ccatctggaa cacggcgggc
     4441 agcgtttcaa tcagcaacgt cgccttgatg gtgccgcgcg gcagattaaa gcgatcttct
     4501 gcatagctga agacttcgct ccaccaggcc gcttcctgcc aggactgggt tttcggcaga
     4561 tagaaatagg gaccgctgcc ctttgccaac agtgcctgat agttgtggaa gaaatagagc
     4621 gcaaaatcaa acaggctacc ggggattgcc tcaccacgcc aggtgacatg tttttccggc
     4681 aagtgcagac cgcgtacccg acaaatcaaa accgctggat tgggcttgag ctgataaatt
     4741 ttgcctgctt cattggtata gctgatggtg ccgttaaccg catcgcgcag gttaatttgc
     4801 ccgtcgatca ctttgttcca gtctggtgcc agtgaatctt cgaaatcggc cataaagact
     4861 ttcacattgg cgttcagtgc gttgatcacc atcttgcgct caaccgggcc agttatctcg
     4921 actcgacgat cttgtaagtc cgcgggaata ccacgaattt tccagtccgt atcgcgaatg
     4981 gaagctgttt ccgaaataaa atcaggcaac gttccgttat caatatcttg ctgctgctga
     5041 atgcgcgctg ccagaagttt attgcgttgt ggcgtaaaat gcgtcaccag ctcagtcaga
     5101 aattctaccg cttcggcagt aagaatttgc ttctcctgct cgccatacgg ccttatgaaa
     5161 gccagttcat cggttgttgt tgcctgttca gtcatcgtgc agctcctcgt catggatccg
     5221 aaaacttccc cactgtgaac gaaggatcgt tgtgcgcttt tcgttcagca caactaagac
     5281 tactcattta aaatgcaaaa tcaaaaacaa tttccatttt aattaaaaat acttgataac
     5341 ttattgataa caatataaat aaaatttatt cacctcctga ggtgcgtttc ggaaataaaa
     5401 aaggcacccg aaggtgcctg aggtaaggtg ctgaatcgct taacgatcga ctatcacaga
     5461 agattaatcc agcgttggat tcatgtgccg tagatcgtat ggcgtgatct gatagacgta
     5521 atagttgagc cagttggtaa acagcaaatt accgtgacta cgccagctcg ctcgcggtgt
     5581 attttgcgga tcattgtgcg ggaaatagtt atacggtact tccgggccta gtccggcttc
     5641 cacatcgcgg aaatattcct gcgccagcgt ttgcgcatca tattcgggat ggcccgtcac
     5701 aaaggcaatg cgcttatctt tgctggcaaa cagatatgca tccccttctt ccgtctctgc
     5761 cagaatttcc agatcggtgt aatcacgaat caacgctgcc ggaaagtcag catagcgcga
     5821 atgtggtgcc aggaatgaat catcaaagcc acgcgtcaga agcgcatgag gatggagaat
     5881 atgatgctcg taaacgccag agagtttgtc ggtgcgagtt tgcttaggaa tgccgtagag
     5941 gatattgagc gcggcctgta ccgcccagca gacaaacagc gtcgaggtga cgtgatcttt
     6001 cgaccactcc agcacctgtt tgatctgcgg ccagtaagcg acatcattaa actccaccag
     6061 gcccagcggc gcaccggtta caatcagacc gtcaaagttc tgttcctgaa tatcttcaaa
     6121 gttacagtag aagttgttca gatgctctgc gggcgtgttg cgcgattcac gggaatcgat
     6181 gcgcaacagc tgaatatcga cctgcaaagg tgagtttgaa agcaggcgca gaaactgatt
     6241 ttcagtttca atcttcttcg gcatcaggtt aaggatcaga accttaagtg gacgaatttc
     6301 ctgaccagac gcacgagaag ttgtcatcac aaagacgttt tcttcacgca agaaattgac
     6361 ggcgggtagc tcgtccggca cacgaatagg cataacctga ttacctcact acatacgctt
     6421 atacgtttag acatccagat agctgaagat aaccagaaaa tttgccaatg tcgagcctgc
     6481 atgttgaagg tgagaaagtt tcaaggaaaa ggctacgtta gaatataaga atgacgaaaa
     6541 ggagagaaaa tggttattag tattcgccgc tcacgacatg aggaagggga gggactcgtt
     6601 gcgatttggt gtcgatctgt cgatgccact cacgattttt tatcagcaga gtatcgggcc
     6661 gagctggagg agctggttcg ttcctttctg ccggaagcac cgttgtgggt cgcggttaat
     6721 gagcgggaac agccggttgg gtttatgttg ttaagtgggc agcatatgga tgcgctgttt
     6781 atcgatcctg atgtgcgcgg ctgcggcgtg ggtcggatgc tggtggagca tgcgctctcg
     6841 atggcaccgg aactgacaac caacgttaat gagcaaaatg agcaggcggt tgggttctat
     6901 aagaaggtgg gttttaaggt tacgggacgc tctgaggtgg acgatttggg gaaaccgtat
     6961 ccattgctga atctggcata tgtcggggcg taggcgtttt tttctcatcc cgctgggctg
     7021 aacttcaggt ttcgtaccca ccaatggtga cacgattatt tctgcttaag cattgccatg
     7081 accgcactat cactgagtac cactgattgc gcatggatgt ggaactggcg atatttcatt
     7141 aacgttgccc cagcgaccac ttcatgaaga atacgttcct caacctgtga caaaccgccc
     7201 tcattcattt ccagcgcgct aacaacaata tccatacgtt ttgctggcaa aaaagaatgc
     7261 caggttgaac gtgggtggag ttgcagaaca agatcatgca gaactttttc tgcaacaaag
     7321 aaattggcga ttaacaggcg ctggttactg aaggcagcgt ctcctgacac ttcacgtttg
     7381 ctttcaagat cgcgaacagt aattttatta cgactaattt ggatatacag aactgacatg
     7441 agattccctt catcatgcaa ataattgata tgcaaaaaac cccggcctta tggaccgggg
     7501 ttttt
//
LOCUS       DBANUU010000183         7410 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000183 DBANUU010000000
VERSION     DBANUU010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.186"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..986)
                     /gene="pdeC"
                     /locus_tag="ACIE68_004222"
     CDS             complement(<1..986)
                     /gene="pdeC"
                     /locus_tag="ACIE68_004222"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFX5910442.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEG"
     gene            1416..1697
                     /locus_tag="ACIE68_004223"
     CDS             1416..1697
                     /locus_tag="ACIE68_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFX5910443.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(1796..2332)
                     /gene="ssb1"
                     /locus_tag="ACIE68_004224"
     CDS             complement(1796..2332)
                     /gene="ssb1"
                     /locus_tag="ACIE68_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFX5910444.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            2586..5408
                     /gene="uvrA"
                     /locus_tag="ACIE68_004225"
     CDS             2586..5408
                     /gene="uvrA"
                     /locus_tag="ACIE68_004225"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFX5910445.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(5443..5799)
                     /locus_tag="ACIE68_004226"
     CDS             complement(5443..5799)
                     /locus_tag="ACIE68_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFX5910446.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(5803..6219)
                     /locus_tag="ACIE68_004227"
     CDS             complement(5803..6219)
                     /locus_tag="ACIE68_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFX5910447.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(6330..7043)
                     /gene="aphA"
                     /locus_tag="ACIE68_004228"
     CDS             complement(6330..7043)
                     /gene="aphA"
                     /locus_tag="ACIE68_004228"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFX5910448.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPVT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVDARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1845 a         1859 c         1865 g         1841 t
ORIGIN      
        1 ccctcctttt ctgccagcgg aataaactct gccggattca ttatttgccc ctgctcacca
       61 agccaacgta acaacgcttc agcgccgata catttttctg ttttgatatc gattattggc
      121 tggtaataaa gacaaagttg atgtttttcg agggcgcgtt gcaatttacg tttgggagat
      181 aaatagtttt gtcgaatacg tagccagagt aatagcagaa caagactccc caaaataccg
      241 gcgggcaacg caaaaataag atgattataa aaatgggtta taagacgttg atatgaagtc
      301 gcaacaatgg ctgcaattgg gcgttttgtc gaataaactg tcgcatataa atagccattt
      361 ctttgtacgg ttaaatcctt caaatgaatc agcggagaaa acgttgctgc cgaggcctct
      421 ttgcttaacg agaaaaaggt tttcgtcacc gtatcataca caccccattg caatgtcggg
      481 tcatcagaca tcacttcact ccagaagaga gggttgataa ccgccacata atttccccgc
      541 tgcatatagg tcattttata gccagagaaa aaaggcgtat cgcggtaata atagatagaa
      601 acgttaggtt cacgcttata atcggccggt gcaatcgtat agccgtttac aggcgctatc
      661 agcgatgagc ataaaaaatg gttatcacgg gcatagatca attcattaat ataaagatag
      721 ccacgaataa tattcaacat tcgcttttga tgggctggag tgcaaacttg cccctgatag
      781 cgttcagctg catcgctcac taaatctgcc tggcgaatga ccagctcgga tttatccagt
      841 gccagttgag caaaggttcg gagctgatta ttcacttctg attttgccca aaggaaggca
      901 atccatagcg aaagaatgat gggaaagaga actaaaaaga taatgcccgg caacgccagt
      961 aattggtgtc gtgcacgatg actcattgct atcaatttcc tgcctgttta tacccggcca
     1021 ttgcaaggtg aaaaccctgg gtaccgacgt ctttagataa atcttacggt ataaatagca
     1081 cttttgcctg atttgaaatt gatacctttc taatcgtcac ctggatgaaa ataattcatt
     1141 gatattcaaa attaatttcc tacaggaatg cagaggcggc gggaagatat gcccttaatg
     1201 ccactgaaaa accacataat ctgtacagca ataaaagtca cggcctaata tggctaacaa
     1261 acaatcaatt gtgatatagt tcacaaaatt aatgaaacaa acagagtgtt tcatttttgt
     1321 gttctgcaag ccccgcgata gccagagtgt accacgcctc ccgtgaacaa cgccgcgctg
     1381 tccagggcat cggctctttt cagaggaatt gtttgatggc taccctcacc actggcgtgg
     1441 ttcttcttcg ctggcaactt cttagtgccg taatgatgtt tctggccagc acactcaaca
     1501 tccgttttcg tcggtctgat tatgtcgggc ttgcggtgat cagcagcggt ctgggcgttg
     1561 tttccgcctg ttggttcgca atggggttgc ttggcatcac aatggcggac atcaccgcta
     1621 tctggcacaa catcgagtcg gtgatgatag aagagatgaa tcagacaccg ccacaatggc
     1681 caatgatttt gacctgatac gtagaagcct ccataacgga ggcttctttt ttatggctgg
     1741 cagatgcttt aatcatccac cttaaaacaa tataacctat tgttttaatg acaaatcaga
     1801 acggaatgtc atcatcaaag tccatcggcg gctcgttaga cggcgctgcc ggagcggact
     1861 gctgcgggcg agactgcgcg ccgccgctga actgattgcc accctgcggc tgctgtggct
     1921 gaccccaacc gccctgcggc tgaccaccac cgatattgcc acctgccgga gcgccaccac
     1981 cctgacgacc acccagcatc tgcatggtgc cgccaacgtt caccacgact tctgtggtgt
     2041 agcgatcctg accggattga tcggtccatt tacgggtacg cagctgacct tcgatataaa
     2101 cctgagaacc tttacgcaga tattcgctgg ccacttctgc cagtttgccg aacagcacaa
     2161 cgcggtgcca ttcagtctgt tctttcatct cgccggtcgc tttatcacgc caggattcgg
     2221 aagtagccag cgtaatgttg gcaactgcgc caccatttgg catgtagcgt acttccgggt
     2281 cctgacccag attaccaacg agaataacct tgtttacgcc tctgctggcc atgttcgtgt
     2341 ctcctgaaaa aaatcgttct gaataagtgt aaacgcgcga ttgtaccatt accaatagcg
     2401 cttttactat gttgtgacct cggttccggg aaacaaacct ggccagacat tgttacacaa
     2461 cactccgggt aatgcattcc aatactgtat attcattcag gtcaatttgt gtcataatta
     2521 accgtttgtg atcgccggta gcaccatgcc accgggcaaa aaagcgttta atccgggaaa
     2581 ggtgaatgga taagatcgaa gttcggggcg cccgcaccca taatctcaaa aacatcaacc
     2641 tcgttatccc ccgcgacaag ctcattgtcg tgaccgggct ttcgggttct ggcaaatcct
     2701 cgctcgcttt cgacacctta tatgccgaag ggcagcgccg ttacgttgaa tccctttccg
     2761 cctacgcgcg gcagtttctg tcactgatgg aaaagccgga cgtcgatcat attgaggggc
     2821 tttctcctgc catctcaatt gagcagaaat cgacgtctca taacccgcgt tctacggtgg
     2881 ggacaatcac cgaaatccac gactatttgc gtttgttgtt cgcccgcgtc ggcgaaccgc
     2941 gctgtccgga ccacgacgtc ccgctggcgg cgcaaaccgt cagccagatg gtggataacg
     3001 tgctgtcgca gccggaaggc aagcgtctga tgctgctcgc gccaatcatt aaagagcgca
     3061 aaggcgaaca caccaaaacg ctggagaacc tggcaagcca gggttacatc cgtgctcgta
     3121 ttgatggcga agtctgcgat ctttccgatc cgccgaaact ggaactgcaa aagaaacata
     3181 ccattgaagt ggtggttgat cgcttcaagg tgcgtgacga tcttacccaa cgtcttgcgg
     3241 agtcgtttga aaccgcgctg gagctttccg gtggtacagc ggtagtggcg gatatggacg
     3301 acccgaaagc ggaagagctg ctgttctccg ccaacttcgc ctgcccaatt tgcggctaca
     3361 gtatgcgtga actggagccg cgactgtttt cgtttaacaa cccggcaggt gcctgcccga
     3421 cctgtgacgg ccttggcgta cagcaatatt tcgatcctga ccgcgtgatc caaaaccccg
     3481 agctgtcact ggctggcggt gcgatccgtg gctgggatcg ccgcaacttc tattacttcc
     3541 agatgctgaa atcgctggca gatcactata agttcgacgt cgaagcgccg tggggcagcc
     3601 tgagcgcgaa cgtgcataaa gtggtgttgt acggttctgg caaagaaaac attgaattca
     3661 aatacatgaa cgatcgtggc gatacctcca tccgtcgtca tccgttcgaa ggcgtgctgc
     3721 acaatatgga gcgccgttat aaagagacag aatccagtgc ggtacgtgaa gaattagcca
     3781 agtttatcag caatcgccca tgcgccagct gcgaagggac gcgtctgcgt cgggaagcgc
     3841 gccacgttta tgtcgagaat acgccgctgc ctgctatctc cgacatgagc atcggtcatg
     3901 cgatggaatt cttcaacaat ctcaaactcg ctggtcaacg ggcgaagatt gcggaaaaaa
     3961 tccttaaaga gatcggcgat cgcctgaaat tcctcgttaa cgtcggtctg aattacctga
     4021 cactttcccg ctcggcagag acactttccg gcggtgaagc ccagcgtatt cgtctggcga
     4081 gccagattgg tgcgggcctg gttggcgtta tgtacgtgct ggacgagccg tctatcggcc
     4141 tgcaccagcg cgataacgag cgcctgttgg gtacgcttat ccatctgcgc gatctcggta
     4201 ataccgtgat tgtggtggag cacgacgaag acgcgattcg cgcggctgac catgtgatcg
     4261 atatcggtcc tggtgcgggt gttcacggcg gtgaagtggt cgcggaaggt ccactggaag
     4321 cgattatggc ggtgccggag tcgttaaccg gacagtacat gagcggtaaa cgcaagattg
     4381 aagtgccgaa gaaacgcgtt ccggcaaatc cggaaaaagt gctgaagctg acaggcgcac
     4441 gcggcaacaa cctgaaggac gtgacgctca cgctgccagt cggtctgttt acctgcatca
     4501 caggggtttc aggttccggt aaatcgacgc tgattaacga cacactgttc ccgattgccc
     4561 aacgccagtt gaatggtgcg accatcgccg aaccggcacc gtatcgcgat attcaggggc
     4621 tggagcattt cgataaagtg atcgatatcg accaaagccc aattggtcgt actccacgtt
     4681 ctaacccggc gacctatacc ggcgtgttta cacctgtgcg cgaactgttt gcgggcgtac
     4741 cggaatcccg tgcgcgtggt tatacgccag gacgtttcag ctttaacgtc cgtggcgggc
     4801 gctgcgaagc ctgtcagggc gacggtgtga tcaaagtgga gatgcacttc ctgccggata
     4861 tctacgtgcc gtgcgatcag tgcaaaggta aacgctataa ccgtgaaacg ctggaaatta
     4921 agtacaaagg caaaaccatc cacgaagtgc tggatatgac catcgaagag gcgcgtgagt
     4981 tctttgatgc ggtgccagct ctggcgcgta agctgcaaac gttgatggac gttggtctga
     5041 cgtacattcg cctggggcag tccgcaacca cactttctgg tggtgaagcc cagcgcgtga
     5101 agctggcgcg tgagctgtca aaacgcggca ccgggcagac gctgtatatt ctcgacgagc
     5161 cgaccaccgg tctgcacttc gccgatattc agcaactcct cgacgtgctg cataaactgc
     5221 gcgatcaggg caataccatt gtggtgattg agcacaatct cgacgtgatc aaaaccgctg
     5281 actggattgt cgacctggga ccagaaggcg gcagtggcgg cggcgaaatc ctcgtctccg
     5341 gtacgccaga aaccgtcgcg gagtgcgaag cttcgcatac ggcacgcttc ctcaagccga
     5401 tgctgtaatc gttaaggccg ctttctgagc ggccttttcc tttcagagtt gtaccagcaa
     5461 tttacgcttt tcttccggta gtaaattcac tgcctgctga taagacgcat ccaccagata
     5521 atagatttgc gaatccggca gcgaaccgtc gagatacacg gtgctccagt gcgctttatt
     5581 cagatggcgg ctaggacgca catcgctgtg ctgctgacgt agcagctccg ccagctccgg
     5641 gctggttttc agcgaaacag ctgggcgatt ttctacttct ttcaccatcg caaacagtac
     5701 atcttccact ttgatctgcg tcgctttcca gtcgttatgc acgctctgtt ctgcgcctgg
     5761 ttttgccatg caatattgta gcaactccga aatggtcatt ttttactccc cttgtagtgt
     5821 cgcgatgatg cgacgcgatc cgccgtggat gcgatgttcc cccagccaga tgccttgcca
     5881 ggtgccggtc tgaatacgcc ctttatgtac cggcaataca agcgatgttc ccagcattga
     5941 ggatttgata tgagaaggca tatcgtctgc tccctcatag tcatgctcat aatttccgtt
     6001 gtcgggaacg gtgcggagga aaaaacgctc catgtcgtgg cgtacggtgg gatcgcagtt
     6061 ctcattaagt gtcagagagg cggaggtatg ttgcagcaac agatgcagta agccgatgtt
     6121 aacgcgcggc atatcagcca gctgattcag aatttcatcc gttaccagat gaaacccacg
     6181 agatttggcg ctaagcgtga gcgtcttttg ataccacatg tgctgctcct tgataaaact
     6241 ctcttaatca gtttgcagca agacagcgaa aggataaagg tgtgattaaa aaaacagcat
     6301 tcgggagagc gtcacgctct cccgctctgt cagtattctg aattgacgat cacctcttca
     6361 ccaaacgcac ccgcttgtgg caagggtttg taggtagagt tggaggcgcg cagaatgcgg
     6421 ataccacgag cgtcgacatc gcgtgcggcg gtaatatcgt tatcggaatc gccataaaaa
     6481 attcggatat ttttatcctg cagccattgc gattttgtat tttgccctgg tttatcgccc
     6541 gcaaagatca ccggattcat gttggtgaca ggaatatgaa aattatccgc cagcgttttt
     6601 gaaaccgttt ctgttttcgt cgggctacga ccagtcacaa agaagatcgc gtcaccgcgg
     6661 cgtacatgca tatcaatcag ctggcgagcg acctcttttg gaatgctgaa ttcatcccag
     6721 ccattgttca ttttttccca gaacacagga tttttcagat aatcttcgct ttctggtgag
     6781 aaggtttttt tgccgcgcca gaagcccggg ctggaaaaaa gtaccgtgtc atcgatatca
     6841 aaccccaccg ccattggcgg acgccctgcg aggctatttt caatttgtgc gaccgaaacc
     6901 caatgaattg gtgcctgttc agcaagcctg gcaacgttag tcccagggtt aagcggtgaa
     6961 ggagatgagg ccagggcaac agcggaactg tttagagcga acaataagca aacggcactg
     7021 attgcctgtg tgatcttgcg catatttttc cctaaatagt cagtatgttg aaacttttta
     7081 ttgtgagatt tgttgcaaaa accatctgac cataacgcca gctgccgggg aaaggaagga
     7141 ttatctgcgg tttttgtgat gaaaagcagg atgattatgt ttaacccagt aaatacgttc
     7201 ctgtcttttt ttgtataaaa aatgtttatt actcgatgta aggatacatt aattttacgt
     7261 ttattatgat gaatcctctg caaatgagcg aaatttgata gaacatttat ctgctgtacg
     7321 atttaatgaa cttaatgata cagcattaca aaaacaatcg aagtttataa agatgattcc
     7381 tgattgacca gaaccctatc ctacaatggt
//
LOCUS       DBANUU010000184         7375 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000184 DBANUU010000000
VERSION     DBANUU010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.187"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(1..4728)
                     /locus_tag="ACIE68_004229"
                     /pseudo
     CDS             complement(1..4728)
                     /locus_tag="ACIE68_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(4748..5209)
                     /locus_tag="ACIE68_004230"
     CDS             complement(4748..5209)
                     /locus_tag="ACIE68_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFX5910449.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5237..7138)
                     /locus_tag="ACIE68_004231"
     CDS             complement(5237..7138)
                     /locus_tag="ACIE68_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFX5910450.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1389 a         2244 c         1983 g         1759 t
ORIGIN      
        1 tcctagtgct tcgtatgctt ctcgtccaat tttcgcaaat tgcttagcat gaatcacttc
       61 atgatacacc tcatataagg tagcattttt cctaagataa atattgcgaa ggccataatc
      121 aaaagctgcc ctagcatttg gtggcaaata tttatctgct ttccttatta ctttaattcc
      181 tagcttctcc atttctttag caatattgtt tatttgacta cgtgataaac gaaccccatc
      241 caggaaaccc gtatgcccac taacacgacg accatcgcca atatagtcca tcaaactgct
      301 gattttagaa cttgcagcca atcccagcgg atcaacccac ccacacgaat ttggcacata
      361 ccggtaagtc tgctcacccc ccagcaaccc cagcggatca ctcaccaggt acatcccggt
      421 ttccggctcg taataccgga accggttata gcacagcccg gattccgcat cctgccactg
      481 tcctgcatac agcaggccgg gggcggcttc cgggtccagt tccccgcgcg ggtccgggta
      541 gccggtgtct gcgggcaggc tgagtgccag cccccacagg ctggtctgcc ccggtcgcca
      601 gacggtttta ccttcactgt taaagagcat cagcggacgg cccgtcaggt cgctcaccgc
      661 atgattcgtc cgcgtcaccc actgcggggc caccgacgga tgcggctgcc gtacccggct
      721 gaactgctgg cttatcagct caaagccgtt aaacaccagg tgccgtacgc tgtacagttt
      781 atcatcgcgg tattcccgga tttccgcaat actgtcgccg tcccacagat acgtgaagcg
      841 gattttcttc cggtcgcagc gtttctctgt tcgtctgccg ctggcgtcgt ggcggtattc
      901 ccacagctca ccctgtgcgc tgcaataccc ggtcagatgg tcacggctgt cccaccggaa
      961 ccgctctgtt tccggtcggt agccgtcacg gtgtctggtg cggctgacca tccggcctgc
     1021 ggcgtcatag tcatactgcg tgtttccggc ctgtttcagc cggtggcctc cggcatactg
     1081 gtcagtctct tcacttatcc ggtgcctgcc cgcagactgc gccttcaggt agccgcagct
     1141 gtcatagcca taggactcat cgccttcgcc atagcccagc ccggtgcctg aggctgtcac
     1201 cgccatgacc tggccgttcc ggtccagccg gtactcccgc cgtgtttccc gtccgtaatc
     1261 ttcccggctg gtgacagccg acacattacc cgcgctgtcg taacggtact cccgtaccag
     1321 tgagggaacc agcccgcccc atgtggcttc ttccggaaaa aatccggcgc gctggctgac
     1381 aggctgaccg gctgcattcc agccctgttc acaggcaaaa ccccggttac tgtgccggag
     1441 cacttcacgt cccgcctcat cacggctgaa cgtcagcgtc tcatcccccg gcaggataac
     1501 ctctgccagt tctccggcgg cgttgtaacg gtaggtggtt ttcagcaggg attcatcggg
     1561 atgtctgtcg cgttcatctt ccggtggcag gatacagcgg atgacggtgt gttcatcggg
     1621 gtagtggtat tcaatggccc gtcggccctg agtggtgaca ttcacccgcc cgcgttcgtc
     1681 atagccggtg cgctgtgata tgccgtccgg gctgaagata cccgtcagcc tgccggattt
     1741 gtcatacgca tactgcgttt tgccttccgg cgtgatgatt tctgccaggc ggcctgcggc
     1801 atcccggctg tagaggatgg tttcctccag accgttaacc tttgcggtat acaggccatc
     1861 ggcgtcatag cggtaacgcc agaccacgcc gccccagtca tgctcttccg tcacccggcc
     1921 gcagttatcc agaacataaa gatatgactc accctgcggg ttgatgactt ctgtcagttg
     1981 cagcgtgtct ttatcgtagc ggtactgcca gcggtggcct tcaccgtccg ttcgcgccac
     2041 cggcaaatcg aacggaccgt attccatcgt ccagacaacg ccgttaccgt ccgtccaggc
     2101 cagcaggttg ccgtggcggt catagcggaa tccttccgcg ccggttccag cacgttcaat
     2161 gccactcagt ttatggtgct gctgccactg taaccgcact gactgaccgc tggggccgcg
     2221 catcttcacc ggcaggtcgt gaccgctgta ttcggtgtgg tgttcacggc ccatcacgtc
     2281 ggtgagtgtt tccggccggt tgtggtggtc gtactctatg cccagacgcc gttcatcctg
     2341 gcggaatatc cccgacagca gcccctgcgg cgtgtacgcc agccgggtct cttcgccctg
     2401 tggcgacacc agacttaccg gcaggccgcg ttcatcacac tgctgcgtcc acgtctgtcc
     2461 ggcagggtcg gtgatgttgc tgagcaggcc gcgctcatca tagcagtact gccactccgc
     2521 gccgtctgcc gccgtgtaac ggcttaactg accgtgtgcg gcgttgtatt caaacttctc
     2581 cacccgcccg gcaggggagt gacgggccag caggtgatgg aattcatccc attaatacgt
     2641 ggtggtgctg ccgtccggcg cttcttcccg cagaatattg ccgtcaggat catagtaata
     2701 acggtgagtg cccttgccgt ccgtcacggt ggtgcagccg tcaccatagt ccagcgtggc
     2761 gttgtaatag ccctccgccc cggtgacggt cacgcaccgg ccctgttcat cgtaggtgaa
     2821 acggctccac gtctggtcgt tatcggacca gcggatgatc cggtccgcag cgtcgtactc
     2881 ataaaacagg tggtagtcca gccgcgcatc cgcttctgtc agccgccccc gcgcatcctg
     2941 ttcgtaggtc gccagcgtct gtatgccgtt atccgtgcgg tgaatggctt tcaggtagcc
     3001 gctggcgtgg tacatcagtt taagccggat cccgtcggtg tgactgacac ccgtgatgcg
     3061 gtactggctg tcgcgctcaa acgtgatgcg gttgctggcg gtgtcgctca tcccgcccag
     3121 catatacatc cggtcgtcgg ggctgtgtgc caggcggtca tcgctggtat cgtcagtcac
     3181 cgggtggcgc agacgcattc ccggaaaggc gtcataaaag aagcggctgc tcagtgcatc
     3241 cctgtcgaac agctccaggt aatcgtcgcg gcggcgcaga atgtactggg cgtgatacgg
     3301 gttaaccgcc gtatcactgt caccgtcgaa ggcgaagttc agcgtggctc cctggctgat
     3361 aatatctacc cggtttccct gttcacgcac ccaggcgtat tcgctccagg agtcgctcca
     3421 gcccacgccg gtcaggccat gcagcggcag tacagaactg tggaaacggg tgaaggagag
     3481 ggggagtgtc tggcccaggg tgaagtcggt acgttcgtcg cagtacgcgc cggttaccgg
     3541 atcgacagga tctgttaccc atctgatgac tttattggca atacttttta acgacttacc
     3601 tgttttcccc agcaacgcgc cgagctttgc ccccagcagc ttcccgcttt tccccagcag
     3661 gttccccagt ttgccgagtc tcgccagatt cttcagtttt cccagcagtc ccgctggcgg
     3721 cagaagatag ctggctatcg ttattccccc caggatcatt ctctgccacg gcgggaactc
     3781 cggctggata tccagatagg tcacctgctc accgccaata aagacgtccg gcgaacctcc
     3841 cacgattgct gcgctgcact ccagtttgtc gccctggcgt gcggcaggct gattgttgat
     3901 aaagacgttg ctgctgccct gggctatcct cggtggtttg ttctccttgt cgcagagcgc
     3961 cgtgtcctca ccggcttccg ggaaatccac atcttttttg atttcgccac gcccgcccac
     4021 catggtggca aactccatta ataccggacc ggcgggaaac agaagcgatg cgccggcgct
     4081 gacgcgatcc ataaaaccgg cattgctgtc cgtcagggtg ataacagcgt tcccccaggc
     4141 cttcgccagt ggtactgctt tttccgctgc cgattcaagc aggtcagtgg caatatcacc
     4201 ccatgagggt tccccctgtg ggacttcagg tattgctcca ggtgggggag gagggggaag
     4261 ggtgacggcg gccctggcag cgggctttcc gttaattttt acgttttcag aaccggtatt
     4321 gatggcgccg tccgtgtcac aaaaactgtt tatccatgac tcaagctggt tctgtttctc
     4381 ctccacataa tcactcgcct tatagctggc atacatcagc gcaggcacca gaaccagcgc
     4441 ataagggccg gtggcacagg cggccataat caggcaacct tctgcgatgg tcactatcgc
     4501 ccccagcacg gcccctgcca gcgcccccaa aaaactcttg tgctgaattt catcgcccag
     4561 cctggcggcg ggggggccag acttttttga tgccagcatc tgtgcaatgg ctttgtcgtt
     4621 ggctgcccgt ttcatctggc tggcgaccat cgcctgctgt gacagcattc gcattgccag
     4681 cgtaccgcct gcggtcgctc cctgtggccc gcctggtcct tcactcatgt gttatccctc
     4741 gtttatgtca gcgcggctgg tccttttgga tgtcattctc cggtggcaac ggggtgaagc
     4801 tctttatcac ctccagcagc atcgtctttt gactgttctt cagggataag gaggtgctga
     4861 aagtgaaggt cagcagcgta tctccacgcc gctggaatac ggctatctgg ctaatccgcc
     4921 cctgattccc ctcatcttcc ggaccgttcc aggaataatc cagcagccag gccggggcct
     4981 ctgccagagt gatttcccgg caggcatgtt cggtgtaccc atcaagtttg ccctggtaca
     5041 acgccagcgt ttcctggtac agctctggtg tcagggttcc ctgtggaacg gcttcgcggg
     5101 taatgaccag gttgatgcca ctgttatctg tggacaccag gacctgcatt gactgatcct
     5161 gccattgaga cggcagttga atgtggcctt cattaaaagt gaactgcata aaatactccc
     5221 tgataatctg agtgaatcag ttgatgaaaa cagaaccgcc cacgatggtg atgccatcct
     5281 tcgacagcct gattaccgtc gattccccga cctgtagctg aatgccatcc ggcgtcagcg
     5341 caattttgct ttcctccaca gccagcaaaa tttccttaat cgccgtataa gcctgtatac
     5401 cgtcgccttc gccaccattg tttacagcgt caacatgcac cgaaccggca atcgtgcctc
     5461 tgtcctgaaa gacatcctca gataaagacc ctttcagtac tccggtgatc ttgtggcctt
     5521 tttccaccgt aagaaggcgg ttaccttcct ggatcgtgac cgtctggtca ttttttacgg
     5581 tttcgcggtg atcgtgtttt acggtggtgg tacggtcgtt gagcacttcg gtatccatgt
     5641 tcttctgcgc gtggatgtag acctgttctc tccctgtcgc atcatcaaac ttcagctcat
     5701 taaatccgct gcccatgtac gttttggagc ggatggtcat ctgcgtcttt gttcccggca
     5761 ggctgccggg ggtgcggttt tcctggtggt aggtgcgccc cataatgatc ggctggtccg
     5821 gatcgccgtt gaggaagtct acaatcacct cctgacccac acgcggtatc gccaggtggc
     5881 caaaaccggt gcctgcccac gcctgtgcca cacggatcca gcacgaactg tcttggtctg
     5941 ccgggttata acggtcccag ttgaacttca cccgcacgcg accatgttcg tcgcagaaga
     6001 tttcctcgcc tgccggtcct gtcacgacag cgctctgcgg gccgtcgacc agcggtttca
     6061 gcaacggctg tggtcgccat gttctgtctg ccgggatcac cgcaaaatgg ttctccagcg
     6121 ccgtccccgc tccctgccgt cctggcaccg cctgtggctg ttcgccgtgc agttcacttg
     6181 ccaccacctg ccattcccgg ttcaggttcg cctgcggatg ccccgtcagc acaattcgcc
     6241 gtcccggcca tatctccggc gagcggctca ttccccgcgc ggtttctgcg ttgtttcgcc
     6301 agccgtccat ctgccagcgg gcaaagttct gcccgtgggc gctcttgaaa cgtccggggt
     6361 agtcatacac ttcatactgc gtgcgctggt agtcctggtg ctggccttcc tgttcaaaac
     6421 gtccggccca gccggggcgt ttaaaggtgt agtctttggt caccacggaa gaagggcgga
     6481 tttgtgcgct gtagcggaac tggctgatgc agagggtgct cacctcggta cgggtgttcg
     6541 ggttccatgg gatttcaaaa gattcgggca gatggcggac tgtgtcgcac agcaccaggc
     6601 tctggtcggt acttttgtaa gcatgctcct cataaaagaa gatgccttcc tccgccgcca
     6661 tccggcacag aaaatcgtaa tcagtctcac cgtactggac acaaaactca cgggaaggat
     6721 gcggctcgct gaacagcgga ctccattcgg tcaccccgtt ttcctgcaac atcgtgccga
     6781 ggatgctttt gatgtcctcg ttctggaaga tacggaagtt ctggcgcaga ccggcacgcc
     6841 acagcggcgg gtgcaccttc atgctgtaca gcatctggtt tttgtcgttc tccccgagtt
     6901 caaaccaggt caccacgcct ttcacccggc gctgtacttc atcgccctgc catatcgtca
     6961 ggtaggccat tttgtccagc acctgcgcaa attcaaggga gagaaactgc tggctgacca
     7021 gggagagatc gagggaaaag agcgaagaga gtgactggtt cagatgaaag gaaaccaccg
     7081 caaaagcatc cggcggcagg ccgtccactt ccagtgtgaa acgtaatccg gttgacatag
     7141 caacctccag taaaatgaat aatgatccgt accgtacaac tgcggataat gtgataagcg
     7201 ggacgacttc agacgttatt agggaaggaa cagaaaatgc tatggttatg tgagaatttt
     7261 gtttaaatat tactttacag aaacgggtgt ctgttcagtc aaaaaaataa tcacaagctc
     7321 aacctcttgt ttcttggcgt tattcaggac aggtacgcat atcacaacga gtaac
//
LOCUS       DBANUU010000185         7290 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000185 DBANUU010000000
VERSION     DBANUU010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.188"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            211..579
                     /gene="sufA"
                     /locus_tag="ACIE68_004232"
     CDS             211..579
                     /gene="sufA"
                     /locus_tag="ACIE68_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFX5910451.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            588..2075
                     /gene="sufB"
                     /locus_tag="ACIE68_004233"
     CDS             588..2075
                     /gene="sufB"
                     /locus_tag="ACIE68_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFX5910452.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            2085..2831
                     /gene="sufC"
                     /locus_tag="ACIE68_004234"
     CDS             2085..2831
                     /gene="sufC"
                     /locus_tag="ACIE68_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFX5910453.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIYMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            2806..4077
                     /gene="sufD"
                     /locus_tag="ACIE68_004235"
     CDS             2806..4077
                     /gene="sufD"
                     /locus_tag="ACIE68_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFX5910454.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            4074..5294
                     /gene="sufS"
                     /locus_tag="ACIE68_004236"
     CDS             4074..5294
                     /gene="sufS"
                     /locus_tag="ACIE68_004236"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFX5910455.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDSEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPRNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            5307..5723
                     /gene="sufE"
                     /locus_tag="ACIE68_004237"
     CDS             5307..5723
                     /gene="sufE"
                     /locus_tag="ACIE68_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFX5910456.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            5872..6876
                     /gene="ldtE"
                     /locus_tag="ACIE68_004238"
     CDS             5872..6876
                     /gene="ldtE"
                     /locus_tag="ACIE68_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFX5910457.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(6940..7176)
                     /gene="lpp"
                     /locus_tag="ACIE68_004239"
     CDS             complement(6940..7176)
                     /gene="lpp"
                     /locus_tag="ACIE68_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFX5910458.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         1845 a         1773 c         1983 g         1689 t
ORIGIN      
        1 ccttaatgct ccttagataa taaccattat ctaacaatga gatacctaat tcttagcgtg
       61 cctgttaacc cacacatcag ggtctatgct tattaaaagc aggcaaaaag catgttttac
      121 ggtaaagccc ctgcgtttgc tgggttgtac tgataatcat tatcactaac atgctgttat
      181 acgctgaaag cgatgaagtg aggtaaatcg atggacatgc attcaggaac ctttaaccca
      241 caagatttcg cctggcaagg cttaacgctg acacccgcag cggcgataca catccgtgag
      301 ctggtggcaa agcagccggg tatggtcggc gtgcgcttag gcgtgaagca aacgggctgc
      361 gcgggctttg gctatgtgct cgacagtgtt agcgagccgg acaaagacga tctgctgttt
      421 gaacacgacg gcgcgaagct gtttgtcccg ctgcaagcga tgccgtttat tgatggcacg
      481 gaagtcgatt tcgttcgtga aggacttaat cagatattca aatttcacaa ccctaaagcc
      541 cagaatgaat gtggctgtgg cgaaagcttt ggggtatagg cggtactatg tctcgtaata
      601 ctgaagcaac tgacgatgtc aaaacctgga ccggcggccc gctgaattat aaagaaggat
      661 tcttcaccca gttagccacc gatgagctgg caaaggggat aaacgaagag gtggtgcgcg
      721 caatttcggc gaagcgtaat gagccggagt ggatgctgaa gtttcgtcta aacgcctatc
      781 gcgcatggct ggagatggaa gaaccgcact ggttgaaagc gcactacgac aagctgaatt
      841 atcaggatta cagctactac tcagcaccat cgtgcggtaa ttgtgacgac acttgcgcgt
      901 ctgaacctgg cgcggtgcag caaactggcg cgaacgcctt tttaagtaaa gaggtggagg
      961 cggcgtttga gcagttgggc gttcccgtgc gggaaggcaa agaggtggcg gtggatgcca
     1021 ttttcgactc agtttcggtt gccactactt atcgcgaaaa actggcggag cagggaatta
     1081 ttttctgttc ctttggtgag gcgatccacg atcacccgga actggtgcgt aaatatctcg
     1141 gcaccgtggt gccggggaat gacaacttct ttgccgcgct taatgcggcg gtagcctctg
     1201 acggtacgtt tatttatgtg cctaaaggcg tgcgctgccc gatggaactt tccacctatt
     1261 ttcgcattaa cgcggaaaaa accgggcagt ttgagcgcac cattctggtg gccgacgaag
     1321 acagctacgt cagctacatt gaaggctgtt ccgctccggt gcgtgacagc tatcagttac
     1381 acgcggcagt ggtggaagtc atcatccata aaaacgccga ggtgaaatat tccacggtac
     1441 aaaactggtt ccctggcgat aacaacaccg gcggtattct caacttcgtc accaagcgtg
     1501 ctttgtgcga aggcgaaaac agcaaaatgt catggacgca atcagaaacc gggtccgcga
     1561 ttacgtggaa atatcccagc tgcattttgc gcggcgataa ctccattggt gagttttact
     1621 cagtggcgct gaccagcggt catcagcaag cggataccgg caccaagatg atccacatcg
     1681 gtaaaaacac caaatcgacc attatctcga aagggatctc tgccggacat agtcagaaca
     1741 gttatcgcgg cttagtgaaa atcatgccga cggcaaccaa tgcgcgcaat ttcactcagt
     1801 gcgactcaat gctgattggc gctaattgtg gggcgcatac cttcccgtat gtcgaatgtc
     1861 gcaataacag cgcacaactg gagcacgagg caacgacatc acgtattggt gaagatcaac
     1921 tgttttactg cctgcaacgc gggatcagcg aagaagacgc catctcgatg attgttaacg
     1981 gtttctgcaa agacgtgttc tcagaactgc cgctggaatt tgccgttgaa gcacaaaaac
     2041 tcctcgccat cagtcttgaa cacagcgtcg gataaggaat aaacatgtta agtattaaag
     2101 atttacacgt cagcgtggaa gataaagcta tcctgcgtgg attaagcctc gccgttcgtc
     2161 ccggcgaagt tcacgccatt atggggccaa acggttcggg caaaagtacc ttatcggcaa
     2221 cgcttgccgg gcgcgaagat tatgaagtga cgggcggcac ggttgagttc aaaggcaaag
     2281 atttgcttgc actgtcgccg gaagatcgtg caggcgaagg catctatatg gccttccagt
     2341 atccggtgga gattccaggc gtcagtaacc agtttttcct gcaaacggca cttaatgcag
     2401 tgcgcagcta tcgcggccag gaaacgctcg accgctttga ttttcaggat ttgatggaag
     2461 agaaaatcgc tctcctgaag atgccggaag atttattaac ccgttcggta aacgttggtt
     2521 tttccggcgg cgagaaaaag cgcaacgata ttttgcaaat ggcggtgctg gaaccggagt
     2581 tatgcattct tgatgagtcg gactccgggc tggatattga cgcattaaaa gtggtcgccg
     2641 atggcgtgaa ctcgctgcgt gatggcaagc gctcattcat cattgttacg cactaccaac
     2701 gcattctcga ctacatcaag cctgattacg tccatgtgct atatcaggga cgaattgtga
     2761 aatccggcga tttcacgttg gtcaaacaac tggaggagca gggttatggc tggcttaccg
     2821 aacagcagta acgcgctgca acagtggcat cacttgtttg aagctgaagg gacaaaacgc
     2881 tcaccgcaag cacagcagca tttacaacaa ttgctgcgta ccggactgcc gacacgtaaa
     2941 catgaaaact ggaaatatac gccgctggaa gggctgacca atagccagtt tgtcagcatt
     3001 gcgggagaga tatccccaca gcagcgagat gccttagcgt taacgttaga cgctgtgcgg
     3061 ctggtatttg tcgatggacg ttacgtgtca gcactgagcg atgcgactga aggcagcggg
     3121 tatgaagtga gcattaacga cgaccgtcag ggggtacccg acgctattca ggcggaagtg
     3181 tttctgcatt tgacggaaag cctggcacaa agcgtgacgc atatcgccgt gaagcgcggt
     3241 caacggccgg caaagccatt gctgttaatg catattaccc agggcgtggc aggtgaagag
     3301 gtcaacactg cccattaccg acatcatctg gatctggcgg aaggtgccga agcaacggtg
     3361 atcgaacatt ttgtcagcct taatgatgct cgtcacttca ccggcgcacg gttcactatc
     3421 aatgtcgcag cgaacgccca cttgcagcat atcaagctgg cgtttgaaaa cccggtcagt
     3481 caccactttg cccataacga tttgttgctg gctgacgatg ccaccgcatt tagccatagt
     3541 ttcctgctgg gtggcgcagt gttacgacac aacaccagta cgcaactcaa tggcgaaaac
     3601 agcacgctgc ggatcaatag cctggcgatg ccggtgaaaa acgaggtgtg tgatacccgt
     3661 acctggctgg aacacaataa aggtttttgt aacagccgac agttgcacaa aactatcgtc
     3721 agcgacaaag gccgcgcggt atttaacggt ttgatcaacg tcgcgcagca cgccatcaaa
     3781 acggatggtc agatgaccaa caataatctg ctgatgggca aactggcaga agtggatacg
     3841 aaaccgcagc tggaaatcta tgcagatgat gtgaaatgca gccacggcgc gacggtgggg
     3901 cgtattgatg atgaacagat gttctatctg cgctcgcgcg ggatcaatca gcaggatgcc
     3961 cagcagatga tcatttacgc ctttgctgcc gaactgacgg aagcactgcg tgatgagggg
     4021 cttaaacagc aggtgctggc ccgaatcggt caacgactgc caggaggtgc aagatgactt
     4081 tttccgtcga caaagtgcgg gccgactttc cggtgctttc gcgtgaggta aacggtttgc
     4141 cgctggctta tctcgacagc gccgccagtg cgcagaaacc aagccaggtg attgactccg
     4201 aagccgagtt ttatcgtcac ggctacgcgg cggtgcatcg cggtattcat accttaagcg
     4261 cccaggcgac cgagaaaatg gagaacgtgc gcaagcgggc atcgctgttt attaatgccc
     4321 gttcggcgga agagctggtg ttcgtccgcg gcacgacgga agggatcaat ctggtcgcca
     4381 atagttgggg caacagcaac gtgcgggcgg gcgataacat catcatcagt cagatggagc
     4441 accacgctaa cattgttccc tggcagatgc tttgcgcacg cgttggcgca gagctgcgtg
     4501 tgatcccgct caatcccgac ggtacgctgc aactggagac gctacctaat ctgttcgatg
     4561 agaaaactcg cctgctggca attactcatg tctccaacgt gcttggcaca gaaaatcccc
     4621 tggcggaaat gatcacgctt gcgcatcagc atggcgcaaa agtgctggtg gatggcgctc
     4681 aggcggtgat gcatcatccg gtggacgttc aggcgctgga ttgcgatttt tacgtattct
     4741 ccgggcataa actgtatggc cccaccggaa ttggcattct ttatgtgaaa gaagccttgt
     4801 tgcaggagat gccgccgtgg gaagggggcg gttctatgat cgccaccgtc agcctgagtg
     4861 aaggcactac ctggaccaaa gcaccatggc ggtttgaagc cggtacaccc aataccgggg
     4921 gcatcattgg tcttggcgcg gcgctggagt atgtttcggc gctggggctt aataacatag
     4981 ccgagtatga acagaatctg atgcactacg cgctatcaca gctggaatct gtaccggatc
     5041 tcactctcta tggcccgcgg aacaggcttg gcgttattgc ttttaatctt ggtaaacacc
     5101 acgcctatga tgttggcagt tttctcgata actacggcat tgctgtgcgt accggacatc
     5161 actgcgctat gccattaatg gcctattaca acgtccctgc tatgtgtcgg gcgtcgctgg
     5221 ccatgtataa cacccatgaa gaagtggatc gtctggtgac cggactgcaa cgtattcacc
     5281 gtctgctggg ataacaggga ggcactatgg ctttattgcc ggataaagaa aaattgctgc
     5341 gtaatttttt acgctgcgcc aactgggaag agaaatatct ctacattatt gagctgggcc
     5401 agcgtctgcc agaattacgc gccgaagacc gaagcccaca aaatagcatt cagggctgtc
     5461 agagtcaggt gtggattgtc atgcgccaga atgcgcaggg aattattgaa ttgcagggcg
     5521 acagcgatgc ggcgattgtg aaagggctta ttgcggtcgt ctttattctc tacgatcaga
     5581 tgacgccgca ggatattgtc aatttcgatg tgcgtccgtg gtttgaaaaa atggcgctca
     5641 cccaacatct caccccatct cgttcacaag gtctggaagc gatgattcgc gcaattcgcg
     5701 ccaaagccgc tgcacttagc taaactaaag ggacagcttt catccggttg gctcacattg
     5761 ccggatggtc aatgtttaca ctgacctgca caggagtgtt ttggtttcag gtgaacataa
     5821 gcgccatccg acgtttcagc gtgagtctcc ggcaaataca ggagatttac aatgaaacgc
     5881 gcgtctttgc ttacactcac gcttatcggc gcttttagcg ccatccaggc tgcctgggcg
     5941 gttgattatc cgctaccacc aaccggaagc cgactggttg ggcaaaatca aacgtatacg
     6001 gtgcaagaag gggataaaaa ccttcaggct attgcccgac gttttgatac tgcggcaatg
     6061 ttgatccttg aagccaataa cactatcgcc ccggtgccaa aacctggtac gacgataact
     6121 attccttcac aactgttatt acctgatgca ccgcgtcagg ggattatcgt taaccttgca
     6181 gagctgcgcc tttattatta tccgccggga gaaaatattg tgcaggttta tccaataggt
     6241 attggattgc aggggctgga aacgccggtg atggaaacgc gtgttgggca gaaaatccct
     6301 aacccaacct ggacgcctac ggcaggcatt cgtcaacgtt cgctggagcg tggcattaaa
     6361 ttaccgccag tcgttcctgc cggaccaaat aacccgctag gacgttacgc actgcgcctc
     6421 gcgcatggta atggcgaata cctcattcat ggtaccagtg cgccggacag cgtcggtttg
     6481 cgcgtcagtt cagggtgtat tcgcatgaat gcaccggata ttaaagcctt gttctccagc
     6541 gtgcggacgg gaacgccggt gaaagtgatc aacgaaccgg tgaaatattc cgtggaacct
     6601 aacgggatgc gttatgttga agtacatcga ccactatcag cagaagaaca gcaaaacgtt
     6661 cagacaatgc catacacact gccagcaggc tttacgcaat ttaaagacaa taaggctgta
     6721 gatcagaagt tagtcgataa agcgttgtat cgtcgggcag ggtatccggt ttcggtgagc
     6781 agtggagcaa ctcccgcagc cagcaatgcg ccttcagtag agtcagcgca gaatggtgaa
     6841 ccagagcaag ggaatatgtt acgcgcgacg cagtagcggt aaacgggaga caaaaaaaat
     6901 ggcgcacaat gtgcgccatt tttcacttca caggtactat tacttgcggt atttagtagc
     6961 catgttgtcc agacgctggt tagcacgagc tgcgtcatct ttagcagcct gaacgtcgga
     7021 acgcattgcg ttcacgtcgt tgctcagctg gtcaacttta gcgttcagag tctgaacgtc
     7081 agaagacagc tgatcgattt tagcgttgct ggagcaacct gccagcagag tagaacccag
     7141 gattaccgcg cccagtacca gtttagtagc tttcattatt aataccctct agattgagtt
     7201 aatctccatg tagcgttaca agtattacac aaagtttttt atgttgagaa tatttttttg
     7261 atgggaaggc acttattttt gatcgttcgc
//
LOCUS       DBANUU010000186         7257 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000186 DBANUU010000000
VERSION     DBANUU010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.189"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..500)
                     /gene="fimZ"
                     /locus_tag="ACIE68_004240"
     CDS             complement(<1..500)
                     /gene="fimZ"
                     /locus_tag="ACIE68_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFX5910459.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSKTLNYIKSNKCSTNSSTITVLSNREVTILRY
                     LVSGLS"
     gene            743..819
                     /locus_tag="ACIE68_004241"
     tRNA            743..819
                     /locus_tag="ACIE68_004241"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:777..779,aa:Arg,seq:tct)
     gene            complement(835..1998)
                     /gene="intD"
                     /locus_tag="ACIE68_004242"
     CDS             complement(835..1998)
                     /gene="intD"
                     /locus_tag="ACIE68_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFX5910460.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHCGIMEDIKKA"
     gene            complement(2130..2309)
                     /locus_tag="ACIE68_004243"
                     /pseudo
     CDS             complement(2130..2309)
                     /locus_tag="ACIE68_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(2225..2530)
                     /gene="yfdT"
                     /locus_tag="ACIE68_004244"
     CDS             complement(2225..2530)
                     /gene="yfdT"
                     /locus_tag="ACIE68_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HFX5910461.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMEWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            complement(2530..2892)
                     /locus_tag="ACIE68_004245"
     CDS             complement(2530..2892)
                     /locus_tag="ACIE68_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910462.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     QENKVIPVSTELPPANESVLLFDAKGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            complement(2883..3419)
                     /gene="yfdR"
                     /locus_tag="ACIE68_004246"
     CDS             complement(2883..3419)
                     /gene="yfdR"
                     /locus_tag="ACIE68_004246"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFX5910463.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSIAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKQMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     LGMFMERFNELSELRKCA"
     gene            complement(3547..4371)
                     /gene="yfdQ"
                     /locus_tag="ACIE68_004247"
     CDS             complement(3547..4371)
                     /gene="yfdQ"
                     /locus_tag="ACIE68_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFX5910464.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKVLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            complement(4437..4799)
                     /gene="yfdP"
                     /locus_tag="ACIE68_004248"
     CDS             complement(4437..4799)
                     /gene="yfdP"
                     /locus_tag="ACIE68_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFX5910465.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGR"
     gene            complement(4814..5125)
                     /locus_tag="ACIE68_004249"
                     /pseudo
     CDS             complement(4814..5125)
                     /locus_tag="ACIE68_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5270..5785
                     /locus_tag="ACIE68_004250"
     CDS             5270..5785
                     /locus_tag="ACIE68_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910466.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGSYQEINASLKAKIAEFENFEAQTEGYILNQLESGTFVYSKEVIVN
                     GGSITMHLCPKCFGQKIVSILQPFPVSEDELFHKSRCLHCENKFLMNKNPDYVSPPSI
                     EELSRKLNGNL"
     gene            complement(6170..7246)
                     /locus_tag="ACIE68_004251"
     CDS             complement(6170..7246)
                     /locus_tag="ACIE68_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFX5910467.1"
                     /translation="MTKSICFFNHKGGVSKTTTTFNLGWALADEGKKVLMVDLDSQCN
                     LTGMVLGYEKIDEGLDSFYSSRDNLTLGPIVEYLINGGQPEAYLEKETGKLHPTLHEN
                     LLLLPGHLDVSDLDSQISVSLKIAAGIPATRNIPGNLPKILQLIAMKNNIDYILYDLS
                     PNVGGLNEVVLMSSDYFIVPVAPDFFCWQAIRSLSKNIPRWHKELALFKENNDGNSSQ
                     SIKNAPQFLGMIQQRYRPRKGAPVKSFEKWMAAIRDAVDSILVPNLEKITCIIPRDKV
                     QHAINKTTVSGDLSAYDLAHISDFNSLIAISQQLATPVFSISDQQIRDSGQFGHALNT
                     MKSSRDAFNEQFKGLAQRVLELTK"
BASE COUNT         1881 a         1647 c         1584 g         2145 t
ORIGIN      
        1 ttagataatc cgctaaccag ataacgtaat atggtcactt cacgattaga tagcacagtg
       61 atcgttgaac tattcgtact acatttattg ctttttatat agttaagcgt tttgctggga
      121 aaaaacgtgt atccggagag gatcatctga acggcatgaa aaatatcatt ctgatcattg
      181 catttactga caaaaccgtt agcaccagct tgtatcgctc tgccagcata aaagcattct
      241 gatttcgatg ataaaaataa cactttcact gtgctctgga tttgtttgat ccttttcagg
      301 aaggtaaaac cgtctgttcc gggcaagtct atatccataa tgattaaatc aacaggacgg
      361 gttcggagat aatcgatggt tatgcggtaa tcatccgttt tcaggacaat ctgcaattca
      421 ctgttttttt gcaacagaac ttcaatagac attctgatga taggatgagt atccataatg
      481 atcaccgacg ttggtttcat agttaccagt ctcataggag cggacaattt tccgttaggg
      541 cggtaaattg tactttgata catgaaaata cgggttttct tgattcagac gcgcagcggt
      601 gtgtgtttgt ttgccgctat agcgaaataa atcagaaaat cagacgcggt cgttcacttg
      661 ttcagcaacc agatcaaaag ccattgactc agcaagggtt gaccgtataa ttcacgcgat
      721 tacaccgcat tgcggtatca acgcgccctt agctcagttg gatagagcaa cgaccttcta
      781 agtcgtgggc cgcaggttcg aatcctgcag ggcgcgccat tacaattcaa tcagttacgc
      841 cttctttata tcctccataa ttccacagtg ggacatattt gggacattat caccaaaaat
      901 gtcgtctatt ttccttgcat gctctgtcaa atgattaggc gcaaggtgag catacctacg
      961 aaccatttct atggactccc atccgcccat ttcctgaagc acagataatg ggacgcctga
     1021 ctggatcagc cagcttgccc aggtgtgtct gaggtcatgg aaacggaaat cttcaattcc
     1081 tgcacgacga caagctgata gccatgatgt cttgctgtcg atgcgcatct tcctgaccgc
     1141 aggcgttgat gttccatctg ctcgcttagc cgccttggta tgtacaaaca cccatttgtg
     1201 atgcttgcct atttgatcac gcaacacttt acaggcggta tcgttcagcg ccacaccaat
     1261 ggcgcggttt gatttgctct cttctggatt cacccaggca actcgtcgct gcatgtcgat
     1321 ttgttgccat tccagattta tgatgttcga ctttctcaga ccagttgcca gcgcaaactt
     1381 gacgacagat ttcagtggtt cggggcactc atcaataagg cgttttgctt cctccttttc
     1441 cagccatctg actcgcttgt ttctgaccgc tggtatcttg atgacaggcg ctttttccag
     1501 ccacttccag tcgcgttctg cagcacggag aatggccttt atcatggcaa gatgctttgc
     1561 ctttgtctga gttgatactg gctttggttc ataaacaggc ggttctttac ctttcctgat
     1621 ggcggcctga actttctgtt tccatatttc tttcgtcttt ctgttatgca ttctgcttac
     1681 agcagagtaa atctttgcct ccgagatatc tttaagcctt ataccctcaa aatgttcaag
     1741 ccagaactca atccggcttt tatctgaatc gagagatttt ttatcagctt tttcctcaag
     1801 ccatcttagg caggcctctt caaaagtgac atcaggtaaa tcccctagct tttctactcg
     1861 ccagagttct gcttttcgct tgtcgtgcaa ctcctgagct tgccgcttgt cctttgtgcc
     1921 aagagattcc ttaattcgtt tcccgcccgg gagcgaatac gaggcatacc atatttcatt
     1981 tctgcggaag agtgacattt tctttcctct gttatgccat cacccgcgct cacctggaca
     2041 gtatgcagcg gagactgaag cgccgcaatg caagcttgcc gtgttgtgag gtaaggagat
     2101 tttggcttgg ttggatcttt acgtgttgcc tgtaggcggc ctgttcgtat ccagttggtg
     2161 gcggttggtc tggatatctt aagaaaatga caggcctcat cgagtgtgag gctgtatggc
     2221 tccattattt cacctcttgc tgtgacattg ttgaaaaatg gataccagct cgttgctgcc
     2281 agacgatcca accgagagtc atatcccatt ccatgtattc gttatcgccg ttttttgctc
     2341 tccgacgatc tactaagtca ccgaaacgct tttccatgaa taattcataa gcttcgcgtt
     2401 catctggttc tacttccaga gataggagtg cgatttcata agcacggcgc tcaatatcgt
     2461 ctcgcacgtc aaggctgctg atacgctctt taatttcttt aatcagttct ttgtcggtaa
     2521 aagtggtcat tatgctccag cctccggtgc ttttggcatt actgcccagt gagtgatatt
     2581 gacgttttca aggtccccga cctgaaatgt ccactgccat tctccggttt ctttttgtcc
     2641 ccaggtgtac cagagagaac gccagccaat tagccagcct tctcctttag catcgaataa
     2701 caaaacactt tcatttgctg gtggcagttc agttgacact ggtattactt tgttttcctg
     2761 tgctgcacat ttagcttcaa gcgcatcgaa tttacgcacc aggtattcag catctgtttc
     2821 atttactttc agatctcgcg gtacacatct cccacgaaga aacccttcca tttcgaaaac
     2881 attcatgcgc atttgcgtaa ctccgataac tcgttaaagc gttccataaa catccccaag
     2941 gcatggcccg gtgccagtgg aatcacgttg aacatctctg ttgccgggat accttccagt
     3001 acaggccaga aagagccatc atcaagcccg agatcgcggc gttcggttgc cagcatgatg
     3061 agatcggcat atttcacggg cgtactcata actgggggta acccgtattt ctcacggatt
     3121 acggcgtcta ttttttcttc catttgttta tagtcaggaa gaaggcgttt cagtggtgcg
     3181 ggaatgtcct ggcaatacgc ttctgttgca tcatgcatta acgcttcaaa agcaaattcc
     3241 tgcggcacca gctggctgca aagaaccgca tgttgggcga tgctgtagaa gtgcgaaaga
     3301 tgaccggcaa agcgacagat atttgaaagg gaaaccgcga tatcgttaat atcgatgtcg
     3361 tctttattta tcctgtcata ataaaaatgc ttcccggaaa aagttttaat aaatgacatt
     3421 ttgttctcca cgtatatgcg ctgcaccgcg ctgaattctg gtaaaaagaa tccctcacca
     3481 tcctgcgatt attgagtaaa ttacgtttcc ataaatgccc ccgcaggggc atttgcagta
     3541 atgaaatcag gcggtgaaag taccaataaa ggtttctact ttgctgtcct tgaatttctc
     3601 aacaagcaga tcacgaaatt cgttagccat ttcttcctgc accgcctcca gctgaataat
     3661 gcgcagaacc agtacaggac gatcgccagt gataatactg aggcgtaatt taaacggacg
     3721 ttctttcaga ccttcaaacg gaacgcattt aaattcaaat gccactggca taatgtcttt
     3781 ggtcttcgct tcgacagact ccatcaggga gcgtttgccg ctgaagtcat tatcttcaaa
     3841 atcagcggtc tggtttgctt caatcgtgat tttacggaca gccgcagccg cttttgttgc
     3901 ctgaatagcg tcaccattag catcaaagcc cacaaggtag tcggcccagt cttcaatcca
     3961 ttctgccagt gacttctggg agttacgctc gccgttaaca gacaacagag cagagaacgg
     4021 tgctgtcttt ttcagtttga gagtggcggt gttatctgcg tgacctggtt catcaatagt
     4081 acccaggtta agcacactga cggcacgcat attatcggca tcgataaagc agcgggtgcc
     4141 ttcatctgca agaactttag aataacgggt aaagtcatcg atgctggcag tggaaagcgc
     4201 accacggaaa cggaagcgat ttaaattaaa tttttccaga tcatgaatgc ggaaattctc
     4261 aggcaatgcc acagcatcgg caccaatctt actgataatt tcattaacac cctgagcaga
     4321 aataagggca tggatttgat taattgcggt tgcgtctaag ttctgagaca taataagtcc
     4381 tcactatata aagatattca gtgatgagat aaataatcag tttattacga acgatattaa
     4441 cgacctgctg cgcggagttt tccgtcaggt tcaccggcaa gagtcagtaa ttgtccctgg
     4501 tcttcctgca gaatagtcag gcgaccaccg cgattgacat acatcggcgt ttcggtggtg
     4561 tcttcttcgg aaattttccc gcggttagtt gggcgaacat atgagagttt gtgtttgatt
     4621 ttcacacggt tctcatcaaa cggttcgatt tccagattga gtgagacctt ccctttggtt
     4681 ttcgtgttca tcacaccgga agcgacctca ctgagaactg cgccgatttt ggtttcaaat
     4741 acgccgccgt ccagctcccc gataaatgcc tgcacatcag tactgcgttc gctagccatt
     4801 ttgctgctcc tcatcatatc gaccctgcaa ggtcggttgg tttctccaca aaacagagaa
     4861 gaacacctgc ggtggcagcc gcccggatgg attgggttat gagcccgtcg tccggtgatg
     4921 ctcttctctg ttttgcaaaa aaggacggta ccagccggaa gcaagggtac aaactggtac
     4981 cgccaggact acacacagca taaagttgtg gtgccgggtg cctcccggtg cctggcgaag
     5041 gttgcacact aggcgggtgg gtatccacag aaggtcgact gtcagcctca accttaaccc
     5101 gcgtgcgctg agccgcattc accacaacgc taaggattct ctctggttga aaatacttag
     5161 ctgttatgtg cctgctttta gccacatcag gcgaggtgga cctggttatt ccccaacaac
     5221 aaggattcgg ttaatctgga tatccccaac aacaataaga gtattcaatg tgatcgctga
     5281 attaacggca gcaatgacgg ctattcgtga aacagcccag attgcaaaac taatgaacga
     5341 ggcaaaaact caagctgaag taaatgcggc tattggtgag ctgaactcaa agcttgcgtc
     5401 tattcagcgc gaatgcgtgt ctctcgttga actggtgggc tcttatcaag aaataaatgc
     5461 ttctctcaaa gctaaaattg cagaattcga aaactttgag gctcagacgg aaggctatat
     5521 ccttaaccaa cttgagtcgg gtacttttgt atactcgaag gaggtaatcg tgaacggtgg
     5581 cagcataacc atgcatcttt gcccaaaatg ttttggacaa aagatagtat cgatacttca
     5641 accattcccg gttagcgaag atgagctttt tcataaaagc aggtgcctcc actgtgaaaa
     5701 taagtttctg atgaataaaa atccggatta cgtatcgcct ccatccattg aggagttgtc
     5761 cagaaaactt aacggcaatc tgtagattac tactgttgtg gatatccaga ttgttaaaga
     5821 gctaagcgtc ctgtagggcg cttttttgtt gctaacgaat catccggtca ttcatacgcc
     5881 accggcggct acttcgtggg cgtcctgcct gtccgtgata gcttacatta tgttgctact
     5941 taaagtagca aaaatcaaca acaaaaagta gatatttgtt gttgtctatg atttttctat
     6001 aattaattga aagttaaatg tttttgctga tttactatgc tgtaaactga aaatatcgca
     6061 cgaagttgga gtaggtattg agcatagtga attatgagga gggagttttt tatgagatta
     6121 atcagggaga aagaagagta aagcccggtt atgcggccgg gcacatgcat tactttgtaa
     6181 gttctagaac tcgttgagcc agccctttaa attgttcgtt aaaggcgtct ctgcttgatt
     6241 tcattgtatt taaggcatgc ccaaattgcc ctgaatcgcg aatttgctga tcactgatgg
     6301 agaaaacagg tgtcgctagt tgttggctta ttgcgattaa tgagttgaaa tcggaaatat
     6361 gcgcaagatc ataggctgat aaatctccac tgactgttgt tttgttaatt gcatgttgca
     6421 ctttatctcg tggaattatg caggtaattt tttctagatt tggcactaag atcgaatcaa
     6481 cagcatcacg gatagctgcc atccattttt cgaaagattt tacaggtgct cctttacgcg
     6541 gcctgtatct ctgctgaatc atacctagaa actgcggtgc atttttaatt gattgtgaag
     6601 agttaccatc attattttct ttaaataatg ctaattcttt gtgccagcga ggtatatttt
     6661 ttgagagaga gcgaattgct tgccagcaaa aaaagtctgg agctacaggt acgataaaat
     6721 agtcacttga catgagaact acttcattta aacctccaac attagggctg agatcataaa
     6781 gaatgtaatc aatattattc ttcatggcga ttagctgtaa tatttttggc aggttccctg
     6841 gtatatttct tgtagcagga ataccagcag caatttttag agaaacactt atctgtgaat
     6901 ctaagtccga aacatctaaa tgtccgggaa gaagcagaag gttttcatgc agtgtagggt
     6961 gtaattttcc cgtttctttt tcaaggtatg cttcaggttg tccgccatta atcaaatatt
     7021 ctacaatagg tccaagagtt aagttatctc tgctagaata aaaagaatct aaaccctcat
     7081 caatcttttc atagccaaga accattcctg ttagattaca ttgcgaatca aggtcaacca
     7141 tcaatacttt cttgccttca tccgcaagtg cccatccaag attgaaggtt gttgtggttt
     7201 tacttacccc acctttatga ttaaaaaaac aaattgattt tgtcatattt ctctatc
//
LOCUS       DBANUU010000187         7240 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000187 DBANUU010000000
VERSION     DBANUU010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.190"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(166..1266)
                     /gene="chaA"
                     /locus_tag="ACIE68_004252"
     CDS             complement(166..1266)
                     /gene="chaA"
                     /locus_tag="ACIE68_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFX5910468.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1536..1766
                     /gene="chaB"
                     /locus_tag="ACIE68_004253"
     CDS             1536..1766
                     /gene="chaB"
                     /locus_tag="ACIE68_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFX5910469.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            1924..2619
                     /gene="chaC"
                     /locus_tag="ACIE68_004254"
     CDS             1924..2619
                     /gene="chaC"
                     /locus_tag="ACIE68_004254"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFX5910470.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2663..3016)
                     /gene="ychN"
                     /locus_tag="ACIE68_004255"
     CDS             complement(2663..3016)
                     /gene="ychN"
                     /locus_tag="ACIE68_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFX5910471.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3201..4595
                     /gene="ychO"
                     /locus_tag="ACIE68_004256"
                     /gene_synonym="iatA"
     CDS             3201..4595
                     /gene="ychO"
                     /locus_tag="ACIE68_004256"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFX5910472.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASIDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLAAPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4596..5247)
                     /gene="narL"
                     /locus_tag="ACIE68_004257"
                     /pseudo
     CDS             complement(4596..5247)
                     /gene="narL"
                     /locus_tag="ACIE68_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
     gene            complement(5240..7036)
                     /gene="narX"
                     /locus_tag="ACIE68_004258"
     CDS             complement(5240..7036)
                     /gene="narX"
                     /locus_tag="ACIE68_004258"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFX5910473.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
BASE COUNT         1855 a         1769 c         1853 g         1763 t
ORIGIN      
        1 aagagtacat tccggctttt caacagctgt tgcagtggtt tcgtgcaggt tgtggtacag
       61 gctcgcaatt ctgattaacg acctgtccag gtagtatgaa gaaatacaat aagcaggaat
      121 ttatattatc tcccccgatt tccgtcatcg gaggagatat aactgtcagg caaatatcgt
      181 catcaaatag gcggcaaaca gtgccagatg cgctgcgcca ttgagcacgt tagtacgtcc
      241 ggtggagaag gagatatggc acagcactaa agaggccacc atcaccacca tttctggcgc
      301 accaagtgca aactgtaatt cgttacccgt cataaaggca attagcgtga cgacaggtac
      361 ggtaagcgaa atggttgcta acactgaacc aaagaacaga ttcatcgcgc gctggacctg
      421 gttgttcaac actgctttta acgcacctaa accttccggc gacagaatca acagagccac
      481 caggaagcca gtaaaggcga caggggcatt catgctgtcg agcaatgtct ccagcgggct
      541 ggcattcatt ttggtcaccg caataacggc aatcagatgg ataatcaacc agatagcatg
      601 ccacacgctg ctatgggcag acggtttacc gtgatgcggg tcatcgtcat cactgtcatc
      661 ttcgtgctcg tagacaaaca aactttgatg cgttttggtc tggatcagca aaaatacgcc
      721 atacattgcc gcagaaatta atgctaccag taacgcctga ccggttgaaa aattcgccgc
      781 aggcagagcc attggaaata ccagtacgat tatcgccagg gggaacagcg caattaaata
      841 ctgcttgata ccaaacagat tcatatattg ggtggcaaac ttacgaccgc ccaacaataa
      901 tgaaaagcca accagcccac cggtaacaat cataatgatt gaatagagcg tatcacgcat
      961 tagcgttggc gcggcgtcgc cggttgccat taaagctgaa atcaaactga cttcaagaat
     1021 aaccactgaa aggctaagaa taagcgaacc gtaaggttct cccaggcgat gggctaatac
     1081 gtccgcatga cggacaacac taaaggcgct acttaaaata ccaataagcg caagaagatt
     1141 gatggcaatg accactggta gtgtctggct gcttccccac aggaacagca ctaccagcgc
     1201 cagaaccggg aaaataagcg aagtctcctt gtggcgggtt tttaccgcct cttgagcatt
     1261 tgacattatg gttatccctt tgcagatgaa tttatcgaaa atgtaaaaaa taggtaggaa
     1321 aaataacaga aagtgtctgg atatcggtaa cattttacga atttttaccc cactgtcgat
     1381 tttttactca ttgggacata aaaataagta ttacgtttag acaatgtttg ttttagcgtt
     1441 tcttaagaac agtctgacat gaaaattctt atgttttggc aagagtagat attgttgacc
     1501 acacttaatg ttcaactttg taaaaggagt caacgatgcc gtataaaacg aaaagcgatc
     1561 tgccggaaag cgtaaagcac gttctaccgt ctcatgccca ggatatctat aaagaagcgt
     1621 tcaacagcgc atgggatcaa tataaagata aagacgatcg gcgtgatgac gccagtcgcg
     1681 aagaaaccgc gcataaagtg gcctgggctg ctgtgaagca tgaatatgcc aaaggggatg
     1741 atgataaatg gcataaaaaa tcgtaaaacc ggtagcttag ttaaagctat tcgtgcggtg
     1801 ttgccttgca agtggtccgt ggattgcata ttgtcccgtt agtggtttca aaatgagcag
     1861 taaaaatgtc cggaagacac caaaaagttg tcgcagggaa gtatgcagtg gcggaggtgt
     1921 aaggtgataa cgcgtgattt cttgatgaat gccgattgta aaacggcatt tggtgccatt
     1981 gaagaatcac tcttatggtc agcagagcaa cgggcggctt cgctggcggc gacgctggct
     2041 tgtcgacctg atgagggacc ggtgtggatc ttcggttatg gatcgttgat gtggaatccg
     2101 gcactggagt ttaccgagtc gtgcaccggt acactggttg gatggcatcg cgcgttttgc
     2161 ctgcgcctga ccgccgggcg cgggactgcg caccagccgg gacggatgct tgcactgaaa
     2221 gagggcggac gcactacagg cgtcgcctat cgactgccag aagagacgct ggagcaggaa
     2281 ctaaccctgt tgtggaagcg cgagatgatt accggctgtt atctgccaac ctggtgtcag
     2341 cttgatcttg atgatggatg cacagtaaac gccattgtgt ttattatgga cccgcgacat
     2401 ccagaatatg aatctgacac tcgcgctcag gtcatcgcgc cgttgattgc ggcggcgagc
     2461 ggtccgctgg gaaccaacgc acaatacctg ttttcactgg aacaggagct tatcaaactg
     2521 ggaatgcagg acgatgggct gaatgatttg ctggtatcgg taaaaaaact gctggcggag
     2581 aattatccgg atggtgtgtt acggccggga ttcgcctgag taaacttccc tcatagtggg
     2641 gcgtcagacg ccccctcaaa cattaaaatg tgagcacttt atcggctgac agcgtccatt
     2701 gcgccagttc cacaagagta ccgatttcca ccccatcaat aagaggaagt gtgctaatcc
     2761 cgcgcccgtc ggtacaggtt ttgcacaatt tcaccggtac attctgagcg gtaaggatct
     2821 ccagcatttg ctgaatgttg tagccttccc ctggtttttg cccgcgcaac ccggctgtga
     2881 ccgcatcaga catcaggaac agacgcagat ccagattgct ctcctgctct cgtaacgcaa
     2941 tggccagccg caagctgtta aacaaggatt cgctcccgta aggtgcgcca ttggcaacga
     3001 tcacgatttt ttgcattatt tactcctgta ttcagggaat tagacattca tcttctatct
     3061 tactgcttct gcagcgtctg accaatcggt cacattttta aggattttcc tgaaagcgcg
     3121 agaaaatacg acaaaagttg ccagtaatcg ttattcttta aggctatggt ttttcattat
     3181 taccggaagt taccgacgtt ttgagccgtt tcgttcctcg cattatcccg ttttatttac
     3241 tcttgcttgt agcaggcggt acagctaacg cacaatctac cttcgagcaa aaagcggcaa
     3301 atccctttga taataacaat gatggtctgc cggatttagg catggctccc gaaaatcatg
     3361 atggggaaaa acactttgct gaaattgtga aagatttcgg cgaaaccagt atgaatgata
     3421 acgggctgga tactggcgag caggcaaaag ctttcgcatt gggaaaagtc cgcgacgcgc
     3481 ttagtcaaca ggttaatcag cacgtagagt cctggctatc accgtgggga aatgccagta
     3541 ttgacgtcaa agtggataac gaaggacatt tcaccggcag tcgtggaagc tggtttgtgc
     3601 cgttacaaga taatgatcgt tatctcacct ggagccagct tggtcttact cagcaggatg
     3661 atgggttggt gagcaatgtg ggcgttgggc aacgctgggc gcgcggcaac tggctggtgg
     3721 gttataacac tttttatgac aacttgctgg acgaaaatct tcagcgagcg ggctttggtg
     3781 ccgaagcgtg gggcgaatat ttgcgactat cggcaaactt ttatcagccg tttgctgcat
     3841 ggcatgaaca gacagccacg caggaacaac ggatggcgcg cgggtatgac ctgacagccc
     3901 ggatgcgcat gccgttctat caacacctca ataccagtgt cagcgtagaa cagtattttg
     3961 gtgagcgtgt tgatttgttt aactctggta cgggttatca caatcccgtc gcgttgagtc
     4021 tgggattaaa ttacacccct gtgccattag tcactgtgac ggcccagcat aaacagggtg
     4081 aaagtggcga gaatcaaaat aacctcgggc tgaatcttaa ttaccgcttt ggtgtaccgc
     4141 tcaaaaaaca actttctgcg ggcgaggttg ccgaaagtca gtcgttacgt ggtagtcgct
     4201 atgataatcc gcagcgaaat aatctaccga ctcttgagta ccgacagcga aaaacgttaa
     4261 cggtgtttct ggcggcaccg ccgtgggatc taaaacctgg cgaaacagtg ccgctgaaat
     4321 tacaaatccg cagtcgttat ggtattcggc aactgatttg gcagggcgat acgcagatat
     4381 taagtttgac gccaggcgca caagccaaca gcgcggaggg ctggacgctg atcatgcctg
     4441 actggcagaa cggggaaggg gcgagcaatc actggcgatt gtcggtggtg gtggaagata
     4501 accaggggca gcgtgtctcc tccaatgaga tcacgctaac gcttgtcgaa ccgttcgacg
     4561 cattgtcaaa cgacgaactg cgctgggaac cgtaatcaga aaatgcgctc ctgatgcacc
     4621 cataccgctg cttcccacgc gagacttgag cttcattttc ttcagcatgt gcttgacgtg
     4681 cacttttact gtgctttcgg tgatgtccag gcggcgggca atcatcttgt tcggcaaacc
     4741 ctgggcaatc agcttgagaa tatcgcgctc gcgtggggtt aactggttaa catcgcgttc
     4801 agtagtggca cggttagcgc gcaagctggc tgccagaaca ggcgttaatg cctcgcttaa
     4861 taccatttcg ccagcagctg cctgatgcaa tgctttcagc agatcttccg gttccatatc
     4921 ttttaataga tagccatccg cgccgcgttt cagtgcggtg accacatctt cttcatggtt
     4981 agagacgctg aataccacaa tgcgccctga gagggacttt tcgcgcagtt tatccagcgt
     5041 ttccagaccg ttcatgccgg gcatattgag atctaacagg atcagatcgg gatcaagaga
     5101 ttccgccagt tcaataccct gttcgccatt actcgcttcg ccaactacgg tgatatctgg
     5161 tgccatactg ataagctgtt tcacgccagt tcgcagcatc ggatgatcgt cgatcagcag
     5221 gatagtagcc ggttcctgat tactcatggg tatctccttg gacgtctgtg aaagtttttt
     5281 cgggaataaa ggtgacaacc acttcggtgc cacctgattc acgacggcga acgcggcaat
     5341 cgcctcgtaa actttgcgca cggtcgcgca ttattatcat gccgtagtga ttgctgcgga
     5401 tggcattttc aggcacgccg cagccgttat cctggacggt cagtttgacc tgattatcgt
     5461 tttgcgccac cgtcaccacg acctcactcg cttgcgaatg tttgagggcg ttacttaatg
     5521 cctcacgggc aatttgcaac aagtggattg cctgatgcga aggcaccaga cgaggcggca
     5581 attgataatc cagcttcacc gggaagccaa atttggcgct gtactcttcg caactcgcct
     5641 ccagcgccgg acgtaatcca ggctcggtga gctgcaagcg gaatgtggtg agcaattcac
     5701 gcaactgcgc ccaggatgca ttcagttcgt tacggatctg acttaacagt tcgcggctgc
     5761 tttctggcag cgcatcgccc tgcatctgta aacaactcac ctgcatcttc atgcaagaga
     5821 gagattgggc aatagaatca tgcagttcgc gcgcaatggt ggcacgctct tccatcacga
     5881 tcaactgttg ctgacgttcc tgatgccgat ccagcgccag cgtggcggtg agttgttcaa
     5941 ccagggtatc caccagttgt tgttgatcat ggctaagatg acgcccctgc ggcagggtcg
     6001 ccagcaaaat accgtactgc gtatgagagt cagccagccg ccacttcagg gtcgtgccgc
     6061 gatcaccaac gggtaatacg ccgcgcgggc agagctggca gcctttatca tcacaagtca
     6121 tatctggctg gcaggtaaac tcctgatgat tctcttcatc atcagtgtca tacacccgca
     6181 gttcgatatc acgtagcagg gttaaattct gtaagccgtt gagtacaggt gacaggcgtt
     6241 cacacagcgg ggcgcgggaa tgcaaacggc ggttagcctg ccataaaaaa gagaggatct
     6301 gatttttatg ctccagcccg gcggttttct cctgaacccg ctgctcaagt acggcataac
     6361 tttcggccag ttctgcagac atattgttca acgcagttcc aagcatcgcc atttcgttgc
     6421 gcccgctgat gtttgcgcgt tgggtaaaat cgcgatggct gacggcactc gccattgcca
     6481 gcagttgccg ccacggttgt agcagtcgcg cccgcaacca gataatagtg aacaccagta
     6541 aaagtgccat aaataccgcc attacccgat ggaccagtac cactgtctca atgcgcattt
     6601 ccgtggtgcg gtcaaaacca gataccagct gatcaagccc ggcaacaaac tggctgacat
     6661 ccgctgacac cgtttctcgg ttttgtgcac gcatcagcgc agggatcagt tcattacgcc
     6721 agtaatcttg taaaccctgt aattgcgcca gttgtccgtc tcgttctgct gctcgagtca
     6781 actcggcgct aaatgccgtt tgttccatct ctttaattaa gggcttgtct ttctcgctta
     6841 atggcactgc cgccaacaga cggtaacttt gcatgcgcag cgatcccgct ttgttgatcg
     6901 catgggcgct gccctgaacg ccttgcacca gccagccaga aaccgccatc cctgccagtc
     6961 caatagcagt agaaagcaac acaataagcg caacctgatt aaccagggtg agcggagaga
     7021 gacaacgttt aagcatgtaa acctcttcct tcaggcttta aatgagcaat aaccttaatg
     7081 aatgtgacga tacaatctgg aatggcagta ttctcggcta ttggctgaag tatacccata
     7141 cccggaaaga gttactcctt atttgccgtg tggttagtcg ctttacatcg gtaagggtag
     7201 ggattctaca gcaccgtgaa aaatctcata atttttatga
//
LOCUS       DBANUU010000188         7178 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000188 DBANUU010000000
VERSION     DBANUU010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.191"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(329..814)
                     /gene="sixA"
                     /locus_tag="ACIE68_004259"
     CDS             complement(329..814)
                     /gene="sixA"
                     /locus_tag="ACIE68_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFX5910474.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(1017..3161)
                     /gene="fadJ"
                     /locus_tag="ACIE68_004260"
     CDS             complement(1017..3161)
                     /gene="fadJ"
                     /locus_tag="ACIE68_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFX5910475.1"
                     /translation="MEMASVFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3161..4471)
                     /gene="fadI"
                     /locus_tag="ACIE68_004261"
     CDS             complement(3161..4471)
                     /gene="fadI"
                     /locus_tag="ACIE68_004261"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFX5910476.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4651..4935)
                     /gene="yfcZ"
                     /locus_tag="ACIE68_004262"
     CDS             complement(4651..4935)
                     /gene="yfcZ"
                     /locus_tag="ACIE68_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFX5910477.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5307..6647
                     /gene="fadL"
                     /locus_tag="ACIE68_004263"
     CDS             5307..6647
                     /gene="fadL"
                     /locus_tag="ACIE68_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFX5910478.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            7013..>7178
                     /locus_tag="ACIE68_004264"
     CDS             7013..>7178
                     /locus_tag="ACIE68_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910479.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIK"
BASE COUNT         1846 a         1894 c         1660 g         1778 t
ORIGIN      
        1 gtttggcatt tatccacttt tgacaatata atatataaac actatagcca gaatttaatc
       61 aagtttcaat tacatcctgc ctgattattc aactctatta tctgtattaa taaatgacaa
      121 tgagtgtcaa acagacatta ttcatttcaa catgtaactt tgggcaaaat caagatacgc
      181 gcagaagaat aatttccgaa tgaatgtaga aagtgtttta tttatagata aggagagtct
      241 tcgatagtag tgaggttgac agatatccgc tgcctgatga gcaattatgc ccaatgaatc
      301 tacctcattg ggcataattt ggaacacatc agatagcttt tgccatcttc agattacacg
      361 ggctcatctg ccagttgaat gtaccgtttc cgctttcatc aagcgttacg ctggcaatgg
      421 ccgaggtggt aaacataggc ggcgtttcgc ccggacataa ctcggcaacc agatacccga
      481 ctaacggtaa gtgagagatc accagtaccg aagcaacacc ttcattggtc aacgcctgca
      541 aataggcact gaccagacca acatcgccgc agggcgttaa ctccggcaga acttctgcac
      601 tggaaggcag gttcagacaa tcccctactt cttccagtgt ttgctcggct cgcaggaacg
      661 ggctcaccag aacgcgttcg atttccactt tttgaccttt cagccagttc gccatcaggc
      721 gagattcgtc acaaccatta gtggtcagag gacgaaccga atcactggcg gcatcgaggg
      781 ctgcgtcgcc gtgacgcatg ataaaaactt gcatattgca ccgcttttgt taaccagatt
      841 cgcccgtttt ctttcatctg aaaccagaga aagaaaacgg tggccggcat tgtgccttat
      901 ccattcaccg aatgaaacgc tgttttttac ctcaatggcg taagtatagt caatatgcgt
      961 ttacattttg accaacactc cgccattcag cgcggattca tatagctttg accttcttat
     1021 tgcaggtcag ttgcagttgt tttccaaaaa ctttccccac gcgcgcccat ctcgaccaaa
     1081 cgctcgcaag gggtaaaacg ggaaccatac tgcgtggcaa gtcgttgcat tattgcaacc
     1141 acctcgcccg cgccgagaga atcgatatag cggaacggtc caccgagaaa tggcggaaaa
     1201 ccaatgccaa ataccgcacc aatatccccg tcacgcacgc tacggataac ctgctcatca
     1261 acacaacgta ctgcttcatt cagcatcaac atcacacacc gttcagcaac ctgcggtgcg
     1321 gagagtcgcc cctgcccttg tgcgccaatg agcgggtaaa tggcgggatc gacctgtttt
     1381 ttgcttttac gccctttctg accataaaga tagaaacccc gaccattttt tctgcctttg
     1441 cgatcgtcgt tcaaaattga agaaacaaca tttgcaggcg cgctaaaacg ttctccataa
     1501 gcggcttcca gtacaggaat aattttagtc ccggtgtcga ttcctacctc atccaaaagt
     1561 tggattggac ctaccggaaa accaaatttc actagcgcag catcaatgtg ctctacccgt
     1621 tcaccttcgg tcagcatgcg gatagcttca ttaatgtaag gcgctaagat gcgattgacg
     1681 taaaacccgg ctttgtcacg cacgacaatg ggcgttttgc cctgtttttt cgccagtttt
     1741 accgtagtgg cgatggtttg cgctgatgta cccgcatgag gaataatctc caccagcggc
     1801 attttttcca ccggactgaa gaaatgcagg ccgataactt gctcaggtcg cgtggcgtga
     1861 gcggcgatat caccaatcgg caaagatgac gtattcgaag caaagatggt atgagcggcg
     1921 caattttgct ccacttccgc caccatctgt tgttttaatt cgagattttc aaacaccgct
     1981 tcaataatca gatcgcgatg ggcaaagccg cgatagtccg tcgttccgga gattaatgcc
     2041 agctgtttgt cacgttcgct ggctttgaga tgacgacggc gaactttgcc ctccagctga
     2101 tcccaactgt acttcagcgc atgatttatg ccctgcgggt tgatatcttt aattctgacc
     2161 ggaagccccg ctttacaagc agtgacataa gcaataccgc cgcccatcaa gccaccacct
     2221 aaaattccca cgctgtttaa tggcgcaggc ggtgcatcac taccgggatc tttcttcact
     2281 tccgtactgg caaaaaagat actacgcaaa gcttgcgatt gtggcgtcat cgccagttcg
     2341 ccaaacgccc gagcttcggc gtcataaccg ctgctggtgc cctgcgctaa tcccgtttca
     2401 acaacctcca ggatgcgttc tgtcgccgga taattgcctt gagttttgtg ttctgttttc
     2461 ttaccgacca ttttgaacag cagcgcacga cctaacggcc ccgccagaat acgctcgcgt
     2521 acaggtagag ggcgggaaga tgggcgatcc ttcttcgcca gctcaacagc gacttccagc
     2581 aaaatggagt gcggaacaac gtcatccacc agccccagct ttaatgcctg tttcgcccga
     2641 agttgttttc cggtgaggat catctctaat gctgtgctga cgcctatcag acgcggtaaa
     2701 cgctgggtgc cgcctgaacc gggtaacaat ccaagttgta cttcaggcaa accgagcacc
     2761 gttttaggat cgtcagtaca aacgcgaccg tggcacgcca gcgccaactc cagcccacca
     2821 cccaggcaag cgccatgaat agccgcgata accggaatgg gcaaagcatg aatctccgcc
     2881 atcaactgtt ggccctgccg cgccagtgct tccgcttctt gcgccgtttt gcagttgccg
     2941 atcatgttga tgtctgcgcc agcaatgaag ttgtccggtt tagcggagac aaacaccacg
     3001 cctcgcaact ctttgttttc acggagttgc ttaataatgg cgcgcacctg cgaggcaaac
     3061 tccgccttca gggtattcat tttctcaccc ggtacgtcga tggtgatgat agcaatgttg
     3121 tccagacgaa cattaagagt aaacactgat gccatttcca ttattccgcc tccagaacca
     3181 ttgccgcgcc aagcccaccg gcagcacagg cggtaactaa accaaatcca ccgccgcgac
     3241 ggcgaagttc atgcaatgtc tgggtaatca tccgcgcgcc ggtcgccgcg aagggatgcc
     3301 cgtaagcaat cgaaccgcca agcacgttaa atttgctatc gtccacttcg ccagtggcat
     3361 gtgcacgccc cagtacttca cgagcaaaac gttcactacc cagcaactga atattcgcca
     3421 gtgtctgagc tgcaaaggct tcgtgcatat cgatcaatgt cagatcgctc attgtcaaac
     3481 cggcacgttc cagcgccagc ggtgttgacc aggccggacc gagcaacatg tcctgccaga
     3541 catcaatcgc agtaaatgcg tagctgcgca gataccccag cggcaccagc cctaattctt
     3601 tcgcccggga ttcagtcatc aggatcaccg ctgccgcgcc atcggtcagc ggcgtactgt
     3661 ttgccgccgt taccgttccg tgtttgcgat caaacgccgg gcgcagcttt gcgtaatcgg
     3721 caagcgagga attaccgcga atattgttgt cttcgacaag cggttgttta taaggaggga
     3781 taaaggcagt catcacctct tctttgagtt ttccgtctga ccatgcctga gcggcacgct
     3841 gatgcgaacg gtgcgctaat gcatcttgct gttctcgggt gatgccgtag gttttcgcca
     3901 tttgctctgc ggtgtcaccc atccgcaagc cggtagaata ttctgctact gcaggcggta
     3961 cgggcattaa atcgcgcaaa cgcaggcgag agaagagttt caggcgctgg ctcatggttc
     4021 gagctttgtt gacatcaacc agcacgcgcg ccagtttttt actgacgcca attggcaata
     4081 cggaagagga atctgcccca ccggcaatcc ccgctcggat agttcccgcc atcaggcttt
     4141 ctgcgacgtt tgcaaccgcc tggaaactgg tagcgcaagc gcggctgacg ctgtaagcat
     4201 cggtatgcac attcattccg gtaccgagaa caatttcacg cgcaatgttg ggggcttcag
     4261 gcatttgtac gacctgacca aagaccagtt gttcaatcac ttcggcgggg atctcgctgc
     4321 gtgccagcaa ttcgcctacc accatcttcc ctaaatcaac cgcgggaatg ccatgaaaag
     4381 ccgtcgcctg acgggcaaaa ggcgtacgta aaccgctaac aatggcgata cgatcgccct
     4441 ggcgggtaac cagcggtaaa acctgaccca taacactccc ctgtaaaaaa ataaataaag
     4501 tggtctgacc tgatcatagt cttaaccatt tttttacatt tagccaagcg gagaaaaagg
     4561 aaagtgggag ctatgacaca gtgaagacag gagaaagaaa agaaaacgcc cctgccagca
     4621 actgacaggg gcgaacgtac ccgagaggaa ttaacgcaga cccagctgga agatcagcat
     4681 ttcagcttcg caggcaaaag tgaaatcgat atccaggcgc acaccgtcag actcttcagt
     4741 gaaagtcggg gagattttgc acggttcgga ttccacgcta cgggcttttt cagtcagcgc
     4801 cgccagcgtt tgttctgctt ctgcgcggtt tgcaaacacg cggctgtaag acgcggtgca
     4861 gtcggagttg tccataatgg tgccaacatc catacagcag caaaccgggg tttcatcagc
     4921 actacattta ctcatcgttg atttcctctg tatgtgcacc caaggtgcca gataaacgtt
     4981 gtggatattt tacgcttccg gaaagtgctg ctccagttgt taattctgca aaatcggata
     5041 agtgaccgaa atcacactta aaaatgatct aaaacaaaat tcacccgaat ccgcgagtgc
     5101 gcaacctcca aattttgcca gctggatcgc gtttcttaga tcatatttga aaaaagatgg
     5161 aaacatactt gcaacattcc agctggtccg acctatactc tcgccactgg tctgatttct
     5221 aagatgtacc tcagacccta cacttcgcgc tcctgttaca gcacgtaaca tagtttgtat
     5281 aaaaataaat cattgaggtt atggtcatga gccagaaaac cctgtttaca aagtctgctc
     5341 tcgcagtcgc agtggcactt atctccaccc aggcctggtc ggcaggcttt cagttaaacg
     5401 aattttcttc ctctggcctg ggccgggctt attcagggga aggcgcaatt gccgatgatg
     5461 caggtaacgt tagccgtaac cccgcattga ttaccatgtt tgaccgcccg acattttctg
     5521 cgggtgcggt ttatattgac ccggatgtga atatcagcgg aacgtctccg tctggtcgta
     5581 gcctgaaagc cgataacatc gcgcctacgg catgggttcc gaacatgcac tttgttgcac
     5641 cgattaacga ccaatttggt tggggcgctt ctattacctc taactatggt ctggcaacag
     5701 agtttaatga tacttatgca ggcggctctg tcgggggtac aactgacctt gaaaccatga
     5761 acctgaactt aagcggtgcg tatcgcttaa ataatgcatg gagctttggt cttggtttca
     5821 acgccgtcta cgctcgcgcg aaaattgaac gtttcgcagg cgatctgggg cagttggttg
     5881 ctggtcagat tatgcaatct cctgccgggc aaactcctca agggcaagca ttggcagcta
     5941 ccgccaacgg tatcgacagt aataccaaaa tcgctcatct gaacggtaac cagtggggct
     6001 ttggctggaa cgccggaatc ctgtatgaac tggataaaaa taaccgctat gcactgacct
     6061 accgttctga agtgaaaatt gacttcaaag gtaactacag cagcgatctt aatcgtgcgt
     6121 ttaataacta cggtttgcca attcctaccg cgacaggtgg cgcaacgcaa tcgggttatc
     6181 tgacgctgaa cctgcctgaa atgtgggaag tgtcaggtta taaccgtgtt gatccacagt
     6241 gggcgattca ctatagcctg gcttacacca gctggagtca gttccagcag ctgaaagcga
     6301 cctcaaccag tggcgacacg ctgttccaga aacatgaagg ctttaaagat gcttaccgca
     6361 tcgcgttggg taccacttat tactacgatg ataactggac cttccgtacc ggtatcgcct
     6421 ttgatgacag cccagttccg gctcagaatc gttctatctc cattccggac caggaccgtt
     6481 tctggctgag tgcaggtacg acttacgcat ttaataaaga tgcttcagtc gacgttggtg
     6541 tttcttatat gcacggtcag agcgtgaaaa ttaacgaagg cccataccag ttcgagtctg
     6601 aaggtaaagc ctggctgttc ggtactaact ttaactacgc gttctgataa cgcgttcgcc
     6661 tggataaagt cacctgcata gcaggtgact ttaactcccc cacttcaccg aaagtagtgt
     6721 cctcattgct tacctcctga gttttgcaaa cagcctgttg gcagcttgcc ctttcaaatc
     6781 aataagcggt atccacagac aaacctgaag ggaaaggcat attttcaggc gttctgctcg
     6841 tccttctcaa agagtttact tttctgcatt tccaggatac tcaacccccc tggctattgt
     6901 gcgctcatac actcaaatta aagataggtt ctaaataaat gagcgttttt tgatagtcta
     6961 tttcattagg taatatatat ttgtaacaaa tcaatcaaaa tggaataaaa tcatgctacc
     7021 atctatttca atcaacaata ccagcgcaac ttacccagaa tccattaatg aaaataacaa
     7081 tgatgaagtt aatggattag tacaagagct caaaaacctt tttaatggta aggaaggaat
     7141 aagcacctgt attaaacatc tacttgagct tataaaaa
//
LOCUS       DBANUU010000189         7175 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000189 DBANUU010000000
VERSION     DBANUU010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.192"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            179..949
                     /gene="yjjP"
                     /locus_tag="ACIE68_004265"
     CDS             179..949
                     /gene="yjjP"
                     /locus_tag="ACIE68_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFX5910480.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNQDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            940..1413
                     /gene="yjjB"
                     /locus_tag="ACIE68_004266"
     CDS             940..1413
                     /gene="yjjB"
                     /locus_tag="ACIE68_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFX5910481.1"
                     /translation="MGVIEFLFTLAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSESLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1521..2060
                     /gene="dnaT"
                     /locus_tag="ACIE68_004267"
     CDS             1521..2060
                     /gene="dnaT"
                     /locus_tag="ACIE68_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFX5910482.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2063..2800
                     /gene="dnaC"
                     /locus_tag="ACIE68_004268"
     CDS             2063..2800
                     /gene="dnaC"
                     /locus_tag="ACIE68_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFX5910483.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2849..3343
                     /gene="yjjA"
                     /locus_tag="ACIE68_004269"
     CDS             2849..3343
                     /gene="yjjA"
                     /locus_tag="ACIE68_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFX5910484.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3597..5888
                     /gene="opgB"
                     /locus_tag="ACIE68_004270"
     CDS             3597..5888
                     /gene="opgB"
                     /locus_tag="ACIE68_004270"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFX5910485.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6025..7047)
                     /gene="lgoD"
                     /locus_tag="ACIE68_004271"
                     /pseudo
     CDS             complement(6025..7047)
                     /gene="lgoD"
                     /locus_tag="ACIE68_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
BASE COUNT         1784 a         1885 c         1789 g         1717 t
ORIGIN      
        1 gtgataattt gtttgcggcg agagataatt ccctttttat caccgagctt gtcccgataa
       61 ccaggattaa tcagcgcaat tttgatctta acctaccgca aaactaccgt gcttcatgat
      121 acaaatggat tctcattatc tgaacaatac gcagcacgtc tatgacaaag ggcgagttat
      181 gcaaactgag caacagcgag ccgtaacacg gctttgtatc cagtgtggat tatttctttt
      241 gcaacatggt gcggaaagcg cgttggttga tgagctttcc tcacgactgg gtcgggcgtt
      301 ggggatggac agcgtcgaaa gttctatctc ttcgaacgcc atagtgctga caaccattaa
      361 agatgggcaa tgcctgacat cgacacgtaa aaatcaagat cgcggcatta atatgcatgt
      421 ggtgactgaa gtccagcaca ttgtgattct ggcggagcac catctgctgg attacaaagg
      481 cgtagagaaa cgatttagcc aaattcagcc attacgttac ccaagatggc tggtagcctt
      541 aatggttggc ctttcctgcg cctgtttctg taaactcaat aaaggtggct gggatggtgc
      601 cgtcatcacc ttctttgcca gtacggccgc gatgtatatc cgccagctgc tggcacaacg
      661 tcatcttcat ccacagatca acttttgcct taccgctttc gccgccacca ccatttccgg
      721 attgcttttg caactcccta ctttcagcaa tacccccacc atcgcgatgg ccgccagcgt
      781 tctgctgctg gtgccgggct ttccgttgat taatgccgtc gccgatatgt ttaaaggcca
      841 catcaatacc ggactggcac gctgggcgat cgccagtctg ctgacactgg ctacctgcgt
      901 cggcgtggtg atggcactga cgatttgggg gctacgcgga tgggtgtgat cgagtttctg
      961 tttacattgg cgcaggatat gatcctcgcc gccattccag cggtcggctt tgcgatggtg
     1021 ttcaacgttc ccgttcgggc gttacgctgg tgtgcgctgc ttggcgcgat tggtcatggt
     1081 tcacgaatga tcttaatgac cagcgggctg aatattgagt ggtcaacttt tatggcttct
     1141 atgctggtcg gcaccattgg tattcaatgg tcgcgctggt atctggcgca tccgaaagtg
     1201 tttaccgtgg cggccgttat ccctatgttc cctggcatat cggcttatac cgcaatgatt
     1261 tcggcggtaa aaatcagcca gttaggttac agcgagtcat tgatgattac cctgttaacc
     1321 aactttctta cagcttcatc gattgttggt gcgttatcca tcggtctttc cattcctgga
     1381 ttatggttgt accgcaagcg ccctcgcgta taaaattgcc tcgcttaaag cacacggatg
     1441 agagacagcc tcctctcctc catgtgttac tataaaagtc atctcccttc tcgttcatcg
     1501 ttccatattt gagaaacagt atgtcttcca gagttttgac cccggacgtc gttggtattg
     1561 acgccctggt acacgatcac caaaccgttc tggcaaaagc tgaaggcggt gtggttgccg
     1621 tatttgctaa caatgccccg gcgttttatg ccgtcacgcc tgcacgcctg gctgaactgc
     1681 tggcgctgga agaaaagctg gcgcgtccgg gaagcgatgt cgctctggac gatcaactct
     1741 atcaggaacc gcaagccgct cccgttgctg tacccatggg gaaattcgcc atgtatccgg
     1801 actggcaacc cgatgccgac tttatccgcc tggcggcgct atggggcgtg gcgctaagag
     1861 agccggtgac caccgaagaa ctggcctcat tcattgccta ctggcaggcg gaaggtaaag
     1921 tctttcacca tgtacagtgg caacaaaaac tggcgcgcag cctgcaaatc ggccgtgcca
     1981 gcaacggcgg actgccgaaa cgagatgtga atacggtcag cgaacctgac agccaaattc
     2041 caccaggatt cagagggtaa cgatgaaaaa cgttggcgac ctgatgcaac gcctgcaaaa
     2101 aatgatgcct gcccatatca aacctgcgtt taaaacgggt gaagaactac tggcctggca
     2161 gaaagaacaa ggggcgatcc gctccgccgc tctcgaacgt gaaaatcggg cgatgaaaat
     2221 gcagcgcacc tttaaccgct ccggtattcg tccactgcat cagaactgct cctttgagaa
     2281 ctatcgcgtt gagtgtgaag ggcagatgaa tgcgttaagc aaagcgcgcc agtatgtcga
     2341 agagtttgac ggcaacatcg ccagcttcat cttttctggt aagccaggaa ccggcaaaaa
     2401 ccatctggcg gcggcaatct gcaacgagct gctgctacgc ggtaaatccg tattgatcat
     2461 caccgtggcc gatattatgt cggcgatgaa agataccttc aggaatagcg gtaccagcga
     2521 agaacaactg cttaacgatc tgagcaacgt tgatctgctg gtgatcgatg agatcggcgt
     2581 gcagaccgaa tcgaaatacg aaaaagtgat catcaaccag atcgtcgatc gccgctcttc
     2641 ttccaaacgc ccaaccggga tgctgaccaa cagtaatatg gaagagatga ccaagttgct
     2701 gggcgaacgc gtgatggacc gtatgcgcct gggtaacagt ttgtgggtga tcttcaactg
     2761 ggatagctac cgtagccgtg taacaggtaa agagtattaa tccccgtttc tggcggtata
     2821 ctgcgacaca attccacctt gagtaatgat gatgaaaact gttaaacatc ttctgtgctg
     2881 tgccattgcc gccagcgcat taatttccac cggggtgcat gctgcgtcct ggaaagatgc
     2941 gctctccagc gccgccagcg aacttggcaa ccaaaacagc acgacacagg aaggcggttg
     3001 gtcgctcgcg tcattaacta acttgcttag cagcgggaac caggccttaa gcgcagataa
     3061 catgaacaac gccgcaggca ttctgcaata ctgcgcgaag caaaagctgg cttcggtaac
     3121 cgatgccgaa aacatcaaga atcaggtgct ggaaaagctg ggcctgaaca gtgaagagca
     3181 aaaagaagac accaactatc tggacggtat tcagggtttg ctgaaaacca aagatggtca
     3241 gcaactcaat ctggataaca tcggaacgac tccgctggca gaaaaggtga aaaccaaagc
     3301 ctgcgatctg gtgttaaagc aggggctgaa cttcatttcc tgaaccgcga aacgccgcgt
     3361 tttagctgtt acccggctga cgcggctctc tcctgccctt ttccctgctc ctggacggtt
     3421 ttccccctgt tacacgggaa gcgaccgcga tcaaaaaagt ctgtttataa actaattcct
     3481 aacaacatcg catttttttg acactagaat tgcagcaata tggcgacgcc attacattgt
     3541 gcttctcaaa aagcttaaac gttggctaaa cggcccggtt tagtgaggat cgcccgttgt
     3601 cagaactact ctctttcgcc ctttttctcg cctctgtgct gatttacgca tggaaagcgg
     3661 gacgtaacac ctggtggttt gcagccacat taacggtgct ggggctattt gtcgttttaa
     3721 atatcaccct gtttgccagc gactatttta ctggcgatgg tattaacgac gcggttctct
     3781 ataccttaac caacagcctg accggtgctg gcgtcagcaa atacatcctg ccgggtatcg
     3841 gcattgtgct ggggctgaca gcggtgttcg gtgcgctggg ctggatcctg cgccgtcgtc
     3901 gtcatcatcc gcaccatttt ggttacagcc tgctggcgct cttactggcg ctgggttcag
     3961 tggatgccag cccggcattt cgtcagataa cggaactggt gaaatcccag tcacgcgacg
     4021 gcgacccgga ctttgcggct tattataaag agccgtcgaa aactatccct gacccgaaac
     4081 tcaacctggt ttatatctac ggcgaaagtc tcgagcgaac ctattttgat aacgaagctt
     4141 tcccggatct cacgcctgaa ctgggcgcgt tgaaaaatga aggcctggat ttcagccaca
     4201 cgcagcagct gccaggaacg gattacacga ttgcgggcat ggtggcttct cagtgcggca
     4261 taccgctgtt tgcccccttt gaaggcaacg cctccgcctc tgtctccagc ttcttcccgc
     4321 agaacatctg tctgggcgat atcctgaaaa actcgggtta tcagaactat ttcgtgcagg
     4381 gcgcgaatct gcgttttgcc ggtaaagatg tgttcctgaa atcgcacggc ttcgaccact
     4441 tatacggctc agaagagctg aaaagcgtgg tggccgaccc gcactatcgc aacgactggg
     4501 gattctacga cgataccgtt ctcgatgaag cgtggaaaaa gtttgaagag ctttcccgct
     4561 caggtcagcg attctcactg tttaccctga cggtcgatac ccatcacccg gatggtttta
     4621 tctcgcgcac ctgtaaccgc aaaaaatatg attttgacgg taagcctaat cagtcattca
     4681 gcgcggtaag ttgcagccag gagaacatcg cgacgtttat caacaaaatc aaagcgtcac
     4741 cgtggtttaa agataccgtc atcgtcgtct cttctgacca tttagcgatg aacaacacgg
     4801 cgtggaaata cctcaataag caggatcgca ataacctgtt ttttgtcatt cgtggcgaca
     4861 agccgcagca agagacgctg gcagtgaagc gtaacacgat ggataacggc gcgacggtgc
     4921 tggacattct cggtggcgat aactatctcg gacttggtcg tagcagttta tccgggcagt
     4981 cgatgtcgga aatcttcctc aatatcaaag agaaaacatt ggcgtggaag ccagatatca
     5041 tccgcctgtg gaaattccct aaagagatga aagagttcac catcgaccag cagaaaaaca
     5101 tgattgcctt ctcgggtagc catttccgtt tgccgctgct gttgcgggtt tcagacaaac
     5161 gcgtggaacc gctgccggaa agcgaatact cagcaccgct gcgtttccag ctggccgatt
     5221 tcgctccacg cgacaatttc gtctgggttg accgttgcta caagatggca caactctggg
     5281 ctccggaact ggcactttcc accgactggt gtgtctcgca ggggcagctt ggcggtcagc
     5341 aaattgttca gcatgttgac aaaacaacat ggaagggcaa aacggcattt aaagatacgg
     5401 tcatcgacat ggcgcgttac aaaggcaatg tcgatacgct gaagattgtt gataacgata
     5461 ttcgctacaa agccgacagt ttcatcttta acgtcgccgg tgcgccagaa gaggtgaaac
     5521 agtttagcgg gatttcccgt ccggagtcgt ggggccgctg gtccaacgcg cagctgggcg
     5581 atgaagtaaa aatcgagtac aagcatccgc tgccgaagaa atttgacctg gtgattaccg
     5641 ccaaagcata cggcaataac gccagccgtc ctattccggt acgcgtaggc aatgaagaac
     5701 aaactcttgt gctgggcaat gaagtgacca ccaccacact gcatttcgat aacccaaccg
     5761 atgccgacac actggtaatt gtgccgccgg aacctgtctc aaccaacgaa gggaatatcc
     5821 tcggacactc gccgcgtaag ctcgggatcg gcatggtgga aattaaagtg gtagaacgtg
     5881 aagggtaatt cctgactgat gtgtaggtcg cataaggtgc gacctacaca ccttctacgg
     5941 cttacaacac attattaaga aacaaaaaaa cagcgcccaa cacttccccg gacgctgcat
     6001 ggcaacttta ataatctcgc cttatcagaa agtaattaca cccttaatca aatcacgatt
     6061 gttaatcaca tcgcgctcgt aggtttctgc cagcgtggca aacggatagc gatgggttaa
     6121 catcatgtca gcagtgattt tcccttccgc catcagtcga ccgactttag cgaaatcttc
     6181 cggcgtggcg ttgcggctgc ccatcatcgt cgtttctttt ttatggaatt ccggatcgga
     6241 gaactgcaac tcacctttaa acaggccgac aaataccacc gtgccgccgt gacgaatcag
     6301 attcacggtg ttattcatcg catgttgatt acctgtcgcg tcgatcactt tctgcgccag
     6361 cgaaccacca aactgcgccc gcagctgcgc gtcaaaatct tcggctgacg ggtccagcac
     6421 gggcaactcc agacgcgttg ccacatgttc acggcgcgcc ggactggtat ccgccaccac
     6481 cacctgtgcg ccatcggctt tagcgattgc cgccgcgccc agaccgattg gccccgcccc
     6541 gaccaccagc acctgctcgc cgggagcaat ggctgcgcga cgcaccgcat gagcgctaat
     6601 agcgaaaggt tcaatcaatg cggccgcctg cgggtcaata ccgtctgcgg gcaaaatgtt
     6661 cgccaccggc acgctcaaat actcactaaa accgccatcc tgatgcacgc cgatgactga
     6721 aattttttca cagcaattgg tacgaccgct tttacacgct gggcattgct gacaggcaac
     6781 ataagggatc acagcaactt gctgaccatt tttaagatca gcaatatttt aacccagccc
     6841 aacaatctcc ccacatattt catggcctaa aacccgtgga taactaaaaa atggttgatg
     6901 tccaccccag gcatgaatat cggtaccgca aatcccgaca gacttaattt ttattaatgc
     6961 ttcattgtca cccggaatag gtatctcgcg ttgtttccag actaattctt tcggctgctg
     7021 gcaaattaaa acattcatcg tagacatgat tcggctctcc attttttgtt gccttagata
     7081 tcaatgaaaa tgaaaaatgg cagcggaacg gaaaatctgt tttgtgaaaa cacacgcata
     7141 aaaatgtttt aaatcgggtt ttaatggact cacaa
//
LOCUS       DBANUU010000190         7044 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000190 DBANUU010000000
VERSION     DBANUU010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.193"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(88..765)
                     /gene="narI"
                     /locus_tag="ACIE68_004272"
     CDS             complement(88..765)
                     /gene="narI"
                     /locus_tag="ACIE68_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFX5910486.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(765..1475)
                     /gene="narJ"
                     /locus_tag="ACIE68_004273"
     CDS             complement(765..1475)
                     /gene="narJ"
                     /locus_tag="ACIE68_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFX5910487.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1472..3010)
                     /gene="narH"
                     /locus_tag="ACIE68_004274"
     CDS             complement(1472..3010)
                     /gene="narH"
                     /locus_tag="ACIE68_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFX5910488.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3007..6750)
                     /locus_tag="ACIE68_004275"
     CDS             complement(3007..6750)
                     /locus_tag="ACIE68_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFX5910489.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAAEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         1570 a         1977 c         1834 g         1663 t
ORIGIN      
        1 ataagcgaag cgcatccgac aactcacccc gcaccgtgcc cataaaaaaa ccccgccgaa
       61 gcagggtcta tgtgaactaa aattcgctta gtggcgagcc cgcaccagct gatacttacg
      121 tgtcagatac tccaccggta cgctccagat gtgcaccaga cgcgagaacg ggaacagcag
      181 gaataacgtc atccccagca ccaggtgcag acggaagata aacgccacgc catcaagatg
      241 ttgagaagca ccaccgtgga aggtcactac cgactgcgcc cagccaacca gtttcatcat
      301 ctcgctaccg tccatatgct gggcggagaa cggaatggtc aacaggccca gcgcgcactg
      361 gataacgagc agcgacagga tcaggatatc cgctccggta gtcgttgcac gcacgcgtgg
      421 gctgaacaga cgacgcttaa gcaacagcac gccgccaatc agacacagta cgccgctggc
      481 accaccagca aacatcgcca ttttctgttt cacttcaatc ggcagccagg cttcatacat
      541 ccagtgcggc gtcaacatac caaagaagtg accgacaaaa atccccagaa taccgatatg
      601 gaacaggttc gacgccaggt tcatcccttt gcgatccagc atctggctgg acgccgcgcg
      661 ccaggtgtac tgcccgtagt cataacgcag ccagctacca atcaggaaga ccgccccggc
      721 tatgtacggg tagatatcaa agaagaacat attcaggaat tgcattagtg ctgtcctccg
      781 gtggtgatat tcagatattg cggcgcgacg gcaccagcaa agcgacgctg atgagcagtg
      841 attgctgaat caccgcagcc tttgtcagca aagaatttaa cctgctcttc ttcccagaca
      901 gcatccagcg cctgcggcgt atcatcgcgc gcttcgtcgg caattttttc cgccactttg
      961 tcgctgtcga tagcggtatt cgccagtttc agcagcagat caaacagcac ggcataacgg
     1021 ctttcacgct gttgcagacg cgcgctcagc aatgccagaa tcggcgcgat atctttcaaa
     1081 ccttccacgg cttcgctttg cggcagctgc gccaggtact ccagatacag cggcagatgg
     1141 tccggcaatt cgcggctgtt taactgcaag ccgtgctgct cgtactgcgc cagcaggtcc
     1201 accatcgcct ggccgcggtc gcgggattcg ccgtgcacat gttcaaacag caacagtgac
     1261 gtggcgcggc cacggtcgaa cagttcgctg tactgggcct gggcatcgag cggatccatc
     1321 gccgttaaat cgcgcaggaa aatgcccagc gcatgggcat cctcttttgg cagatttttc
     1381 gacgcggcaa tcgcctcaaa catctcttgt tgatgctgcc ataaggcagc atccggatat
     1441 tcaaggagac gcgatacaat cacgagttcg atcatggatg cggctccgtt ttgctggtca
     1501 catcgatggc atcgatacga cggctgttga acagattgaa tttggtatct gaaccgtggc
     1561 agccatcacc aaaggtaaag ccgcagccat ttttctccgg gaaggcttcc cgtgccagtt
     1621 cacgatgact actcggcacc acaaagcgat cttcgtagtt agcaatcgcc agataacggt
     1681 acatctcctg tgcctgggct tcggtcagac cgacctcttc cagcgcacgg gtatcaactt
     1741 taccgtcaac ggtttcagca cgtttgtaat gacgcatcgc cagcatacgt ttcagcgcgc
     1801 gcagtaccgg tttggtatca ccggcggtca gcagattcgc cagatactgt accggaatac
     1861 gcaggctttc gacgtctggc agaatgccgt tgctacccag ctcacccgcg tctgctgcag
     1921 actgaatcgg agacagaggc ggcacgtacc agaccatcgg cagtgtgcga tattctggat
     1981 gcagcggcag cgccagtttc cattccattg ccattttata aaccggcgac tgctgtgcag
     2041 cttcaataac gctcagcgga ataccgtctt taatcgcctg ctcgatgact ttcggatcgt
     2101 tcggatcgag gaacacgtcc agctgacgct ggtaaagatc tttctcgttc tcggtgctgg
     2161 ctgcacgttc aatcgcgtcg gcatcgtata acagcacgcc gagataacgg atacgaccga
     2221 cacaggtttc tgagcacacg gtcggctgac ccgcttcaat acgcggatag cagaagatgc
     2281 acttctcaga cttaccgctc ttccagttga agtagatttt tttgtatggg catccggtga
     2341 tgcacatacg ccagccacgg catttatcct gatcgatcag gacgatgcca tcttcttcac
     2401 gcttgtaaat cgcaccgctc gggcaggtcg ccacacatgc cgggttcagg cagtgttcgc
     2461 acaggcgcgg caaatacatc atgaaggtgt tttcgaactg gctatacatc gccttctgga
     2521 tgttgtcgaa gttcttgtct ttcgccagtt tgtcaaactc accacccagg tcatcttccc
     2581 agttcggccc tttttcgatt ttcgccatcc gttccccggt aatcagcgaa cgcggacggg
     2641 caatcggctg cgatttgctg ccttccggcg cggtatgcag gttctgataa tcaaaatcga
     2701 acggctcgta ataatcgtcg atccccggca gatgcgggtt agcgaagatt ttacccagca
     2761 gcatggcgcg gttacccatg cgcggctgca gtttgccatt gattttacgg atccagccgc
     2821 ctttgtattt ttcctggttt tcccagtcag tcgggaagcc ctgacccggc ttggtttcca
     2881 cgttgttgaa ccacgcgtat tccacgcctt cacggctggt ccagacgttt ttacaggtaa
     2941 ctgaacaggt gtggcagccg atgcacttat cgagattcag caccatgccg acttgtgaac
     3001 gaattttcat tttacgctct cctgtacctg gtcattgcct tcgccatcta accagtcaat
     3061 gttcttcatc ttacgcacta caacaaactc atcgcggtta gaacctacgg tgccgtagta
     3121 gttaaagccg tatgccagat gggcatagcc gccgatcata tgcgtcggtt tcggcgtgat
     3181 acgggtgacc gagttatgga taccaccacg ctgttgggta atttccgaac caggcaggtt
     3241 aacgatacgt tcctgcgcgt ggtacatcat ggtcatccct gccggaacac gctggctgac
     3301 aaccgcacgg gcagtcagag caccgttgct gttgaatact tcaatccagt cgttatcggc
     3361 gatacccaga tctttggcat cggcttcact caaccagacc accggaccac cgcgacctaa
     3421 agtcagcatc agcaggttgt cgctgtaggt ggagtggata ccccacttct ggtgcggcgt
     3481 caggaagttg agcgcttttt ccgggttgcc gttggatttc tggcctatca cttctttcac
     3541 cgaacgggtg tcgatcggcg gacgataaac cagcaggctt tcaccgaaat cacgcatcca
     3601 ctggtgatcc tgatacagtt gctgacgacc agagagcgta cgccatggga tcagctcgtg
     3661 aacgttggtg taaccggcgt tgtaagaaac gtgttcatct tccagaccag accaggtcgg
     3721 gctggagata attttgcgcg gctgtgcctg aatatcgcgg aagcggatct tctcgtcttc
     3781 tttattcagc gccagatgcg tatggtcacg accggtaaat tcgctcaggg cagcccaggc
     3841 tttcacggct acctgaccgt tggtttccgg tgccagtgtc aggatcatct ctgccgcatc
     3901 aattgcggtg ttcagcatcg gctggccttt cgccggacct tccgctttgg tgtagttgag
     3961 cttacgcagc agatccatct cgctctgggt gttccaggca atccctttac cgccattacc
     4021 gattttctcc atcagcgggc cgatagaggt aaagcgttcg taagtcgccg gataatcgcg
     4081 ctctacgacc ataatgtgtg gcgcggtttt acctgggatc aggtcgcact cgcctttttt
     4141 ccagtctttc acatccagcg gctgcgccag ttcagcggca gagtcatgct ggataggcag
     4201 cgtgacgatg tcggtttctt tacccagatg gccgacgcac acttcggaga atttcttcgc
     4261 gatggctttg tagatttccc agtcgctttt cgcttcccag gccggatcga ccgccgcaga
     4321 cagcgggtga ataaacggat gcatatccga agtattcatg tcgtcttttt cgtaccaggt
     4381 cgccgtcggc aaaatgatgt cggaatagag acaggtgctc gacagacgga agtccagcgt
     4441 aaccaccaga tccagcttgc cttccagacc attgtcctgc cagtccactt cttccggctt
     4501 cacgccgccc tgttgcccca gatctttacc ctggataccg tgctccgtcc ccagcaggta
     4561 cttgagcata aactcatgac ctttaccgga agaaccgagc aggttagaac gccagatgaa
     4621 caggttgcgc gggtggtttt taccgttttc tggttgttct gccgcaaaac ggatggaacc
     4681 ctctttcagg gatttcaccg tatagtccac cggattcatc ccggcttttt ccgcttcggc
     4741 agcgatagtc agcgggttag tgcctaactg cggtgcagac ggcagccagc ccatgcgttc
     4801 cgcacggacg ttaaagtcga tcaggtgtcc ggtataacgg gatttatccg ccatcggtga
     4861 cagcaactct tccgccgtga cggtttcata acgccactgg ctggagtggt tatagaaata
     4921 agaagtgctg ttcatgtgac gcgccggacg ctgccagtca agggcaaacg ccagcggctg
     4981 ccagccggtt tgtggacgca gtttttcctg acctacatag tgcgcccagc cgcccccgct
     5041 ctgaccgaca cagccgcaga aaatcagcat gttgatcaga ccgcgatagt tcatgtccag
     5101 gtgataccag tgattcagcc ccgcaccgac gataatcatc gaacgaccgt gcgttttatc
     5161 agcgttatcg gcaaattcac gggcgatgcg aataatctgg ctgcgagaaa cgccggtaat
     5221 ctgctcggcc caggccgggg tataagcttt cacatcgtca tagctggttg cacagttaac
     5281 gtcgttcaga ccacgttcca gaccgtagtt tgccagcgtc agatcataaa cggtggtcac
     5341 cagggcggtg ctgccatcag ccagttgcag gcgtttcacc ggcagtttgt gcagcagcac
     5401 gttttccagt tccactttgt tgaagtgttc cgtgccgtcg ccaccaaagt acgggaagcc
     5461 tacctcagcg atctcatcct ggctacccag caggctcagt tgcaactcgg tttcttcgcc
     5521 agttttgcca tcgcgctgct caagattcca tttgcccttc tcgccccagc ggaagccaat
     5581 agaaccgttc ggcgcaacca tttcgccatt ggtattaaag gcgacagttt tccattccgg
     5641 attgttttcc tggcccagcg catcaaccag atcagcagcg cgcagcatac gacctgcagc
     5701 gtagtaaccg tcgcgttctt ccagcatcac cagcatcggc atgtcggtgt agcgacgcac
     5761 atagtcggtg aaatactggc ttgggttgtc gaggtggaat tcacgcagca ttacgtggcc
     5821 catcgccagc gccattgctg catcggtgcc ctgtttcggt gccagccaca gatcgcacag
     5881 tttggcgatt tcagcgtagt ctggtgtgac ggcaacagtt ttggtgcctt tgtaacgcac
     5941 ttcagtaaag aagtgagcat ccggggtacg cgtctgcggc acgtttgagc cccaggcgat
     6001 gatgtagctg gagttgtacc agtcagcaga ttccggtacg tcagtttgct cgccccaggt
     6061 ttgcggagac gcaggaggca ggtcgcagta ccagtcgtag aagcttaagc aagtaccgcc
     6121 aatcagcgag agatagcgtg cacccgatgc gtaagaaacc atcgacattg ccggaattgg
     6181 cgagaaacca gcaacacggt ccgggccgta gtttttgatg gtgtaaacgt tagatgcggc
     6241 gatcagttcg ttcacctcct gccaggaaga acgaacaaaa ccaccgcgtc cacgcgcctg
     6301 cttaaagctt ttcgctttat cggcgtcttc aatgatagaa gcccatgcct caaccggatc
     6361 gctatgcagc gccttcgctt cacgccacat tttcatcagg cgtttgcgca tcatcgggta
     6421 tttcaggcgg ttggcactgt aaagatacca ggagtagctg gcaccgcgcg ggcagccgcg
     6481 aggttcatgg tttggcagat ccggacgggt acgcggatag tcagtctgct gggtttccca
     6541 ggtgaccaga ccgtttttga cgtagatttt ccagctgcag gagccggtgc agtttacccc
     6601 gtgggtagag cggacgattt tgtcatgctg ccaacgctgg cgatatccat cctcccagtc
     6661 acggttggta ttgagaagct ggccatgccc atcggcaaag gtttcaccct tctgtttgaa
     6721 gtagcgaaac cggtccagga atttactcat cggttttctc ctgtgggagc ctgtcggccc
     6781 tctgataaat cgacattgct aatgtttaag ggcaaggtaa cgctctgaaa cagcgtggga
     6841 attgataacg atcaagagtg atggggatga aaaataaagt aaattcctaa gatacccctt
     6901 taggataatt aacagccaca gcataacaga ctgtaaatta agacgtttta cacctgtagt
     6961 tatttaaact attaagaatt ttatgcaggt gggtattaag gagtattccc catgggtaac
     7021 atattaatgg ctcttaatga aatc
//
LOCUS       DBANUU010000191         7043 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000191 DBANUU010000000
VERSION     DBANUU010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.194"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(60..2843)
                     /locus_tag="ACIE68_004276"
     CDS             complement(60..2843)
                     /locus_tag="ACIE68_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFX5910490.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2900..5272)
                     /locus_tag="ACIE68_004277"
     CDS             complement(2900..5272)
                     /locus_tag="ACIE68_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFX5910491.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGRLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFANQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5310..6995)
                     /locus_tag="ACIE68_004278"
     CDS             complement(5310..6995)
                     /locus_tag="ACIE68_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFX5910492.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         1826 a         1614 c         1582 g         2021 t
ORIGIN      
        1 acataaaaat agcgaatatt gctaaaatcc ccgccaacga tgtgttgacg gggctgttat
       61 tattttggca ataatactcc ggtataagta tttaccggat gagaaagata ttgtttaacg
      121 gcagtgttaa cattctctac cgtcatttgt ttcaacaatt gctcctgctc agtccatgct
      181 gcaggatcgt catattgaat aagactattt acaatagtgt tcgctaattg ttgaacgcta
      241 cgctgttgga tatcgaggct gcgctgaacg ttttgctggt attcattcag ttcttgctca
      301 ctgatccctt tagccagacg cttaaccatc acttcattcg ctaacgttaa cagttcatca
      361 tgtcgttctg gttgacaagt aaaagccagc aaatgactga tatctttggc ctgaggatca
      421 accgagaggc gagaagaaac gctgtatgct ccagatgcct gttcacgaat attaacacgt
      481 agatcttttg ccagtgcgac gttaaaagca tcgagcgcca tacgcgtcgc cagattaaca
      541 ggtgtccggg aatcataacg cttccactgt gaaacctgtg ccacaggttc attttgttct
      601 tttacagtaa ccgatgcgtt gtccgtcgcg cgagttaatg gtttacctgc ggctaatggc
      661 gaatcagagt gtttgattga tcctaagtaa cgcgtaatta acgccacgag tttgtcttct
      721 gagacattac cgacaatgac aaacgtgata tccgctggag atgaaaacaa ctggcgatcg
      781 gcagccagcg catctgcggc agtaaactgt acaatctgat tttcttgcag taatttcgtg
      841 cggtcatcag catagcgcgt ctcatacatc tgctgggcaa atttctccgc tggacgctgg
      901 tcgagcgttt tcaacgcctg aatttgagca ttttgtagcg atgcccaaat attgtcgtta
      961 atcgtgctgt gggtgattcg ctggttaatc aactgaaaac caggttcagg gttattagtc
     1021 cgcgcgctaa cggagagcaa cgtattcatg ccactgactt tactgctcat ggttaccgaa
     1081 ttttccgcac tccagcgttt caggctggaa gaggagagct caccaacgcc gcttccgctt
     1141 actgctttat ttgccagcgc gataagtgat ttttgctgcg cagggaaact taaatcgcct
     1201 ttattcgata cggcagtaat ttgtagcttt tgctcttcac ccgcggattt tgccagaata
     1261 accctggcac cattggaaag tgttaatgat gtcagattct cagccagcgt ttctttgctg
     1321 ctaatttccg cctgtgggtc agcgtctact gttaacgata aatttctgcc tgggaagatg
     1381 taagccgcca gctttttgtt ggcgtactct ttttccagcg ccaggatagc cgcaggagac
     1441 aatgcttttt tggcggcaag ctcattgttt accatttgct cccaaaatgc gtcctggttc
     1501 tttcttaact gctgccattt ttccgccaga ctttgcacgg taatttgctg ccacagacgt
     1561 ttcgaaagtt gatatgtctc ttccggcgac aagaacggcg tattatttaa tgagctggat
     1621 gccaggcgac tggtcagcat acgtaaatca cgttcagctt gctgatcaac cgcatttttc
     1681 agccaggtga ggcgggtaga tttgacatca tcgagttctt cagcagaaaa accatgctga
     1741 tcaatggttg ccaactctgc cattaatgca ttcgcagcat cctgcatatt atcgtctcgt
     1801 gcatttacac ggaaaaacag cgactgataa tcgggtgcaa ttttaacgct gcgcgcagtg
     1861 ccgccagaaa tagtcttcaa ctcgcccgac tgtatgcgtt cctgcagacg ttgattgaac
     1921 agctgaacta acatgctcca ttcagcttgt tcgacaaagc tttgctcatc gttcacttgt
     1981 accattggca ggcgataata gagtgcgatg ccgttcaccc ggttttcttt atcattaatg
     2041 atattaaagc gcaggtggtt ttcggctttt gtcggccaga cgcgattttc agctgctttg
     2101 ttagccggaa gcttacttaa attatccttt atcagcgcca gcgcttcttt actgtcgata
     2161 tcgccgacca cgataaaggt catattattt ggttgatacc agcgttgata aaattggcgc
     2221 aattgtgccg gtgtgaccgt ggcgacagta tccatcaggc cgataggttc acggtctaaa
     2281 ttacgggtat ttgccagcag gaaagggcgg cgcgcctgag aggtgcgcca tttcgcatcc
     2341 tgatgggcac gccattcctc agtaattacg ccacgttcag cgtctacttc gagtttttca
     2401 aaggttgcgg cattactcca ttcactgaag attgccatca cttgttgcag attttgtttc
     2461 tgcgtagtcg gcaaactcac ctgatacacc gtttcgtcat agctggtata ggcattaaca
     2521 tcgcgaccaa aacgcaggcc cattgactca aatgtttcga tgactttatt acccggccat
     2581 gtttttgtgc cgttaaacat catatgttct acaaaatgag ccacgccgcg ctcattgtct
     2641 tcttcctgca atgaaccggt atgaatttgc agccataaat ttacctgatc ctttggatga
     2701 gcatgcggat aaatcatata tcgcaagcca ttgtccagtt gcccggtaat taacttttca
     2761 tcctgcggta aggcggcggc aatcagccgc ccggggagca acagagttgc cactaacgtc
     2821 agtaagaaac agaggtttct cataattatc tccatgcgaa aaccgggcga atttacccgg
     2881 ttaagtaaaa tccgaactat taaaatttca tgctgacatc aagccagaaa gtacggccac
     2941 tggcgtaggt cgccacaccg gtgttcgttg tatcaacagc ggttttgcta tccagtacat
     3001 tgagaatatc ggcactgatc gtcaggtttt gttgtttcag aaattgtggc gtccaggaca
     3061 agcgggtgtc ccaggtcagg ctgctatcca gtttttcgtc aacatactgc ttgtaatctg
     3121 aatattcgct gatgtattgc gcatttgtct taccaaggat aatgcgagct ttacgcgctt
     3181 cttgccaggc cagcgtattc gcccacacta acccgctcgg ttgatgcgtg aaatccatgt
     3241 ttaaggagat ctttaatggg ttattaaaat ctgccactgg aacgctatcg taagagacca
     3301 gattaccgtt ataaaccacc tggttatcac cggtattgct ctcctcataa ccattgttta
     3361 acgacaagtt gcccttgctc ttgatatagc taaagacaat ttgtgggtta atatctacct
     3421 ggcggatatg caggggttcg gccagttcaa aactgaggct gaacgaatgc gttttggttt
     3481 tgccatcgtt gttatattca gtaatggtgg ttttagtcgc gctgtcggta cgactgcttt
     3541 tgctgatttg atcatgcgct tcacggtaaa catagtttgc acgcgcaata acgttcttac
     3601 cgattttctg ctgcaatccc attgccagtt catcgttata aggcgttttc aaatcctgat
     3661 aacgcgtcag agttttatta cctgataccg attccgtcca gctattgcgg atatcacgta
     3721 atcccatatc aagaatattc ccgccatagt aacggttata acctgctgta atcattgagg
     3781 tttgattagc aaaaatatcc cattccgtca taaagcgcgg ggagatattg tggtttgaca
     3841 gatagttgtc atagtcatac cgcacgccgg gcattaatga cacattacgc cagctaatgc
     3901 gatccgccat atacagcgtg tagttgtcaa ttcccaggcg gcctttacct ttatggtaaa
     3961 tggtatggtt agttttcttt ccggcagcat taatcacata ggattcagac tggttatggc
     4021 gttcagtcca cgcatcggaa tagatgtatt ccgcgccgaa gtagggttga tgcgaaacat
     4081 tacccacggc gaatttttgc cagtccaggc gtgttttgaa ggtgtaatta tctacagcct
     4141 gggaaatgtg tcctaatccg ccacgggtgc aacgccctgt aatatcacca tatgtacatg
     4201 aaagttcggt gtaccagatg tcatgatcgt gacgggtata atcactaata tgatcccaac
     4261 caacggtggt acgcagtttg gcccatgcga gctgcgtatc catatcccat gccagaccat
     4321 aggatttatt acccatttcg cgatcagact gcgggaaggt gctggtatta taatcacggc
     4381 tggagccggt atattttaag gttaaatcgt gggtaaagcg gtcgctggca aaccaggtaa
     4441 atttgctcaa tgcagtatcg ataacgtttt tatactgtgc ccgaccggcg acaatgccgt
     4501 cattcgaaac ataatccgcg cgggtgatat cagactggcg gcgcgataaa ccggcggtaa
     4561 cgccgaagtt atcagcgagt tcctgattaa acgacaaggt ataaaagttc tttttaaaat
     4621 ctggggagta ataagtactt cctgaagagc cttgattaaa tgcgctcttg ttattctcat
     4681 cgatatgcga tgttaaccag tccgaacgcg tagtgcgata acccaatttc accttgctat
     4741 catcagcgtt gaagcgtttg atctttgcat caattacccc gccattgaag cgaccaaatt
     4801 caaccggcac aaaactgtca taaagcgtca cattgtccag taagctgaca tcaagataat
     4861 acccctgtga catcccgcta atattggttg cactactggc atcggactca ttcgctgggt
     4921 tcaggttatt agttgcacta ataccgtcaa tcaaataggc attctggtag ggcgacgcac
     4981 cgtgaataga gattttctca gggcgaatat ctccctggtt caacgaggta ctttgcgttg
     5041 aatccatgcg taccgcaggg ttggttctca gcagatcgct gatattgcca ttcccggtcg
     5101 gtaaacgctc gatgctttca ctggtgtaat gcgtgttgcc gtttacgggt acagggacag
     5161 gagcatagac cgtcatctct tcaaaaaaat gcatgtacat gtttttgtca tcgacggcct
     5221 gcgccacatc agcgccacat aaaatgacgc caggaataag aactcgcttc atttatttat
     5281 atctccgtac agcagggctt ttattgttgt tataaaaccg tgctaatgtc acaaatatca
     5341 tcagccaggt tccagacacc gggttgatgt gtaaccataa tgacgccgct tgtgggtagt
     5401 ttttcacgca ctaaacgcag taagcgtata gcctcttgtt cctcaagatg agaggtagtt
     5461 tcgtcaagaa atatccattt cggacgtcgt aaaattaatc gtgccagggc gatacgttgt
     5521 ttttcgccgc tggaaagaat atctccccag cgatcgtggt catgaatacg cgcagccaat
     5581 ttccccagac caacctgatg cagtacttcg ctcaacgatt tatcgtctac gggcagggga
     5641 agtgctttac aaataatctc tttcagtaag ccgcttttga ttaacggtgt ttgtgacaca
     5701 taccaactgt cagcaggaga agaaatatca cctttaaacc acggccagca gtgggataat
     5761 gttttaagca gtgtggtttt tcccgcacca gagtagcctt tcagtaatag ccatttgcct
     5821 ggcgaaacat gaaagttcaa gttctctaat atgatcttat tatcaggcgt acgaatactc
     5881 gcattagcca cttgtaccgc atgttggcaa tttttaggct tattcgtagg gcgctgttca
     5941 gtgagttgat ggaactcata caagcgatcg ataaccgcag ccagctcagc aagttcgtca
     6001 tatttataaa taaaccagct taaattgttc gatactagca taaatgcctg gcgcgatttc
     6061 attagtccgc ccagattgat ctgcccgcta ataaactgcg gcaataacag aaagtaggga
     6121 agaacgctaa gcgagcgcga ataaatattt tgccagtaat caagccaccg ctgacgattc
     6181 attaaacgat gccagttctc tttaatggta tgaaaattgt cgcgcaactc ctggcgttgt
     6241 agagactcag cattgcttag cgcaatcagt tctgcctgct tattatgctg cacaagatta
     6301 gttcgaaatg tcgcttcgct acgttgtttt tccacattaa gcggacgaat acgtttaccc
     6361 accttatggg taaataaagt tccaccgatc acaatgagca caacggtata gaccatatac
     6421 ccttggatat tccattctgt tccaccaaca gtgaatgaga gcgtacccgc gctttgccaa
     6481 agaataacgg taaaggtgat cagcatgcta agtgactgga tgaagccaaa tgaaaggctg
     6541 agcgttttgc tgatcagtaa gagaatgtct tcagcgatac gttggtcagg gttatctgta
     6601 tttttatgct caccgtagat ctgcgtgaag tagtagtttt tatctgcaaa ccaccgatta
     6661 aggtagtaat ctgttaacca ttcgcgccag cgaatagtca gcagtttaat taaccatgtt
     6721 ttgtttacgg atattaatac aaagatcccc agcagggcag ggaaccaaag gacaagctgc
     6781 caaagcttat cggtttcttt ctggcttagc gcattgaaaa aatcattatt ccagtcgttt
     6841 aaccagacct gaattttaac cacgccaagg atcatggcga ggataataat gattaacaac
     6901 actgaagttt tattattctt acgcagccaa aagggcttca gcagacagag atactttgct
     6961 atcaacatac aaaacgtaat gggaatggtt atcattagcg aaaattgatt gtgccatcca
     7021 atgattatct atggcaatga ttt
//
LOCUS       DBANUU010000192         7037 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000192 DBANUU010000000
VERSION     DBANUU010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.195"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(352..2001)
                     /gene="pgi"
                     /locus_tag="ACIE68_004279"
     CDS             complement(352..2001)
                     /gene="pgi"
                     /locus_tag="ACIE68_004279"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFX5910493.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRD"
     regulatory      2238..2434
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2526..3875
                     /gene="lysC"
                     /locus_tag="ACIE68_004280"
     CDS             2526..3875
                     /gene="lysC"
                     /locus_tag="ACIE68_004280"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFX5910494.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAAVQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            4020..4946
                     /locus_tag="ACIE68_004281"
     CDS             4020..4946
                     /locus_tag="ACIE68_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFX5910495.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNHIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYNKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLADKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(4982..5773)
                     /locus_tag="ACIE68_004282"
     CDS             complement(4982..5773)
                     /locus_tag="ACIE68_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFX5910496.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(5779..6906)
                     /locus_tag="ACIE68_004283"
     CDS             complement(5779..6906)
                     /locus_tag="ACIE68_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HFX5910497.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
BASE COUNT         1770 a         1796 c         1665 g         1806 t
ORIGIN      
        1 aatttccctt cattgaatga atggagattt acccaaaaac atttcgggcg ggatcgagta
       61 tacacaacta aagcatgcga attacggcgc ggggaattag agatggggtt ttaggataat
      121 tggaatgaat atcaaatata agctacaact tatatataaa taacattaaa aatcataatg
      181 ttaaaataaa agtaaaagta attaatctga gtattttccg aaatcaatca cgattaaaaa
      241 tctattttag gattaacctc acggtatgat ttccgttaaa tcacagacaa ggggatttat
      301 ctgataaaac aatgccgaca taacgtcggc attgttatta aggtttttaa attaatcgcg
      361 ccacgcttta tagcggttaa tcagaccatt ggtcgagcta tcgtggctgc tgatttcttt
      421 atcatctttc agctctggca gaatacggtt tgccagttgt ttacccagct ccacgcccca
      481 ctggtcgaag gtgaagatgt tcaggatcac gccctgagta aagattttgt gctcatacag
      541 cgcaatcaac gcccccaggc tgaacggagt gatttcacgc agcaggatgg agttggtcgg
      601 gcggttacct tcgaacactt tgaacggcac cacgtagtca agcgttgccg gatctttacc
      661 ctgatcgcga tattcctgct caaccacttc gcgggattta ccaaacgcca gcgcttcagt
      721 ctgagcaaag aagttagaca gcagtttctg gtggtgatca gagagcgggt tatgggtgat
      781 agccggagcg atgaaatcgc acggtaccat tttggttccc tggtggatca gctggtagaa
      841 cgcgtgctga ccgttagtgc ctggttcacc ccagataatc gggccagtct ggtaatccac
      901 aacgttaccg ttacggtcaa catacttacc gttggactcc atattgccct gctggaagta
      961 cgccgcgaaa cggtgcatat actggtcata cggcagaatc gcctcagttt ccgcaccaaa
     1021 gaaattgttg taccagatgc caatcagcgc cagcagtaca ggcaggtttt tctcggcagg
     1081 cgtggtggag aaatgcttgt ccatcgcgtg tgcgccagaa agcagttcaa cgaagttatc
     1141 aaagccgatg gagagaacaa tcgacaggcc aatcgctgac cacaaagagt aacggccgcc
     1201 gacccagtcc cagaactcga acatgttggc agtatcaata ccaaactcgc caacggcttt
     1261 ggcattggtt gagagcgccg caaagtgttt cgcaacgtgt ttttcatcac ctgccgcttt
     1321 caggaaccag tcacgcgcgc tatgggcgtt ggtcatggtt tcctgagtgg tgaaggtttt
     1381 agatgctacc aggaacagcg tggtttccgg gtttactttt ttcagcactt ccgcgatgtg
     1441 agtcccatcg acgttagaaa caaagtgcat gttcaggtgg tttttgtacg gacgcagtgc
     1501 ttcggtcacc atgtacgggc cgaggtcaga accgccgatc ccgatgttca ctacgtcagt
     1561 gattgctttg ccggtatacc ccttccactc accggagata atcgcttccg agaaggtttt
     1621 catcttctcc agcaccgcgt tgacttccgg cattacgtct ttgccatcaa ccagaatcgg
     1681 ggtattgcta cggttacgca gcgctacgtg cagcacggcg cggttttcag tgcggttgat
     1741 cttctcgcca gagaacatcg acttaatcgc gcccgccaga tcgcactctt tcgccagatc
     1801 ctgtaatttc gccagcgtct cttcagtgat gcggtttttg gagtaatcca ccagcatctg
     1861 atcgtcgaat gttgcggaga acttagaaaa acgatcgccg tctttagcaa aaagatcggc
     1921 gatcgtaacg tctttcattt catcgaagtg tttctgtagt gcctgccagg cagcggtctg
     1981 cgttggattg atgtttttca ttagcaatac tcttctgatt ttgagaattg tgactttgga
     2041 agattgtagc gccagtcaca gaaaaatgtg atggttttag tgccgttagc gtaatgttga
     2101 gtgtaaaccc ttagcgcagt gaagcattta ttagctgaac tactgaccgc caggagtgga
     2161 tgaaaaatcc gcatgacccc gtcgttgaca accgccccgc tcacccttta tttataaatg
     2221 tactacctgc gctagcgcag gccagaagag gcgcgttgcc caagtaacgg tgttggagga
     2281 gccagtcctg tgataacacc tgagggggtg catcgccgag gtgattgaac ggctggccac
     2341 gttcatcatc ggctacaggg gctgaatccc ctgggttgtc accagaagcg ttcgcagtcg
     2401 ggcgtttcgc aagtggtgga gcacttctgg gtgaaaatag tagcgaagta tcgctctgcg
     2461 cccacccgtc ttccgctctt cccttgtgcc aaggctgaaa atggatcccc tgacacgagg
     2521 tagttatgtc tgaaattgtt gtctccaaat ttggcggtac cagcgtagct gattttgacg
     2581 ccatgaaccg cagcgctgat attgtgcttt ctgatgccaa cgtgcgttta gttgtcctct
     2641 cggcttctgc tggtatcact aatctgctgg tcgctttagc tgaaggactg gaacctggcg
     2701 agcgattcga aaaactcgac gctatccgca atatccagtt tgccattctg gaacgtctgc
     2761 gttacccgaa cgttatccgt gaagagattg aacgtctact agagaacatt actgttctgg
     2821 cagaagcggc ggcgctggca acgtctccgg cgctgacaga tgaactggtc agccacggtg
     2881 agctgatgtc gaccctgctg tttgtcgaaa tcctgcgcga acgcgatgtt caggcacagt
     2941 ggtttgatgt acgtaaagtg atgcgtacca acgaccgatt tggtcgtgca gagccagatg
     3001 tagccgcgct ggcggaactg gccgcggtgc agctgctccc acgcctcaat gaaggcttag
     3061 tgatcaccca gggatttatc ggtagcgaaa ataaaggtcg tacaacaacg cttggccgtg
     3121 gaggcagcga ttatacggca gccttgctgg cggaggcttt acacgcatct cgtgttgata
     3181 tctggaccga cgtcccgggc atctacacca ccgatccacg cgtggtttcc gcagcaaaac
     3241 gcattgatga aatcgcgttt gccgaagcgg cagagatggc aacttttggt gcaaaagtac
     3301 tgcatccggc aacgttgcta cccgcagtac gcagcgatat cccggtcttt gtcggctcca
     3361 gcaaagaccc acgcgcaggt ggtacgctgg tgtgcaataa aactgaaaat ccgccgctgt
     3421 tccgcgcgct ggcgcttcgt cgcaatcaga ctctgctcac tttgcacagc ctgaatatgc
     3481 tgcattctcg cggtttcctc gcggaagttt tcggcatcct cgcgcggcat aatatttcgg
     3541 tagacttaat caccacgtca gaagtgagcg tggcattaac ccttgatacc accggttcaa
     3601 cctccactgg cgatacgttg ctgacgcaat ctctgctgat ggagctttcc gcactgtgcc
     3661 gggtggaggt ggaagaaggt ctggcgctgg tcgcgttgat tggcaatgac ctgtcaaaag
     3721 cctgcggcgt tggcaaagag gtattcggcg tactggaacc gttcaacatt cgcatgattt
     3781 gttacggcgc atccagccat aacctgtgct tcctggtgcc cggcgaagat gccgagcagg
     3841 tggtgcagaa gctgcatttt aatttgtttg agtaatattt ccctactggc gtataacgct
     3901 caccgattgt tatacgccat cctcactgaa aacatagatt aaataatatc tgaaaagcaa
     3961 aatcactcct gtagaacagt attataatca ccgaaaaata tcatttaaca ggagtgttaa
     4021 tggctacttt aatggaaaaa gatgcacttt taaacggagc cagtcagtgc atcgcgtttt
     4081 tatcaaatat cattgataat tgctcagttt cgtcgcatca ggattcaggc gatgctctca
     4141 agcgattagt cagctatcgc gattatcttt attctacccc cgctgaactt gttgatttta
     4201 cacaaggtaa gattctcctt cagcaggtaa gaactcaata tcagcatgaa ttcaacaata
     4261 ctacgcattc agaaaataaa gcatcttttg attctatttg gcagcgtctg acaaatcatg
     4321 aagtcacacc gcaacagcat cccattggtt ttgttttagg tgggcaaccc ggagcaggta
     4381 aatcatctct gattgaatta gccaaaagag agacaaaaga taatataatg attattaacg
     4441 gtgatgattt tcgatttcta caccctgatt ttaatcatat ctaccaaaat tatggggatg
     4501 attttgttac ccatacagcg aaattttccg gcgaaacggt cgaacgtgca attgagagag
     4561 cgatagtcag taaactcaac attgttgtcg aaggaacatt tcgtaatgcc gctacgccgt
     4621 tacaaacatt aaaaaaactc aaagatgcag gctaccaaac agaagtcatg ataaaaacaa
     4681 cctcagcggc attaagctgg gaaagcacca atgaacggta taataaggac aaagaggcgg
     4741 gaaatattgc ccgaaaagtg gataaaaacc accatgatat cgtcaccggt ttgctggcag
     4801 aaaatgcccg caaggtgttt gcttcaaacc tggcagataa gtttgcggtg tattcgcgtg
     4861 agaagatgat atttagttca caagcggcaa cgaatgacga tattgcaacg cttatacaga
     4921 atgaaatcag cgggaataca caataataat aagagctgat ggcgtgaaat aacgccgggc
     4981 tttatgcccg gcgcattacc gtctggtaca ttaagctatc cagttcctta acgtcccctt
     5041 ctctgctatc cggtaacagc ggtggaattt ccccacgcgc cagttccgca cgaataaacg
     5101 catgtaacaa tggacggcgc ggaccatatt ccgcttctcc tgcgcgctgt ttacgttcca
     5161 gtaattcgtc tatttctgca cgtaacggcg catcaagttc gcttcccgcc agtaactcag
     5221 caaagcgcat tggcggtacg cctttacccg cttcgaccca gcgcacggcc agcagcggac
     5281 gcagcacgta aaagtatttt ttcagtcgaa cttcgtcgcc ctgcaaatat ccggggaagt
     5341 ttttctgcgc catcgaatag taatgccagc gcgcacgcag cggagaaaac cacattggga
     5401 ccattgcctt aaacgccgtt atcgtttctt catcttgttg ataaaccacc ggagaatcca
     5461 gccattctat cagcgtcggg ttcgcgcctt tcaataatcc cagcgcctta cgccactccc
     5521 aaccggaaac atctaactcg tcgtcgatgg gcagctcaat aacatcgcgc ggtgactcta
     5581 ctcgcagata ccattccagc gggtgaacat acaaaaaccg tacatcgtag tcgctgtcgg
     5641 gcgaggcaaa tccccagccg cggctccctg attcacaggc gtacagtact ttcacatcat
     5701 accgccgttc aatttctttt aactgctgcg ataccctttc tcgcatcgca gcactgactc
     5761 catttaatgg catgttactc accctttgat attaaccaac ccatgcagaa cctgatccag
     5821 accgccaaat acagagatgt tatcgatccg ttcagccact ctctccagcg tttccagttc
     5881 ttttaagcgt aacgctaccg ggttgttttc catgacttta gcggtattga gtaacgaacg
     5941 cgtcgctgcc gtttcttcac gtcggcgaat cacattggcc tgcgcggatt tctccgcctc
     6001 aaccagctgc gcaaggatat ttttcatatc gccaggcagg acgatatctt tcacccctaa
     6061 cgaggcaatc tccataccaa atggcaacat gcgcgatttc acctgttcgc tgacaacgtc
     6121 atcaatgacc tgtttatctt ccagcaattc atccaacgta cgcgtaccta ccgcttcacg
     6181 cagggcaaat tgtaattccc gatacaaatg atcaatcggc ttcgttaact ggctaaatgc
     6241 caataacacg tcgctgtagc gccagttagc ggcgaggtta atccgcagat tgaccttatc
     6301 tttcgttaag atttcttgcc cgctgacttc cagtacctgc aagcgggtgt caaccacttc
     6361 agcgtcaacc agatggttaa ttttccagta tgcggtcagc cccggcggta acagcgcctg
     6421 cgtttcacca tcgattttca gcacgcctac atgccaggcc ggaacctgca ccgttagtat
     6481 tgcatcgcgg cctttaactg cccctttgcg ccgtggttgc aataccgcat tcatcacctc
     6541 cgtttgtacc tggacttgct gcgtattcat ccgcaccagt gtcaaatcat cctctacacg
     6601 ccagtacaaa cggcgcgttg atggcggcag gatctcctgc aaaataccgt ccatatacaa
     6661 cgccccggct tcaatctccg acaagtctgc caccaggcaa tatttctcca cccaatcagg
     6721 ctgaaagcgg cgcaaataat ccgccagcac gtcgggaact tcgctgccat cgagcgtaat
     6781 cagcaagact tctggcgtat ttaaccaggg taaaagatgc tcgcctgcat gtagcacttt
     6841 gtagtaatcg ccgtttctgg agagtaatgc cagctgatct tttcttacgc ttattttttt
     6901 aatcattatc tattccttgc ttaacagccg tcgcggaagc agacacgaag cgacagggga
     6961 attccctttt gcctgttacc cggcatcttt atcggcgacg ttatcgggga tcgctgactg
     7021 cgcaatccct cactttt
//
LOCUS       DBANUU010000193         7037 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000193 DBANUU010000000
VERSION     DBANUU010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.196"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            573..1562
                     /gene="araF"
                     /locus_tag="ACIE68_004284"
     CDS             573..1562
                     /gene="araF"
                     /locus_tag="ACIE68_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFX5910498.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1632..3146
                     /gene="araG"
                     /locus_tag="ACIE68_004285"
     CDS             1632..3146
                     /gene="araG"
                     /locus_tag="ACIE68_004285"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFX5910499.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3161..4147
                     /gene="araH"
                     /locus_tag="ACIE68_004286"
     CDS             3161..4147
                     /gene="araH"
                     /locus_tag="ACIE68_004286"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFX5910500.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4314..5114
                     /gene="otsB"
                     /locus_tag="ACIE68_004287"
     CDS             4314..5114
                     /gene="otsB"
                     /locus_tag="ACIE68_004287"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFX5910501.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAAVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5089..6513
                     /gene="otsA"
                     /locus_tag="ACIE68_004288"
     CDS             5089..6513
                     /gene="otsA"
                     /locus_tag="ACIE68_004288"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFX5910502.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6520..6948)
                     /gene="uspC"
                     /locus_tag="ACIE68_004289"
     CDS             complement(6520..6948)
                     /gene="uspC"
                     /locus_tag="ACIE68_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFX5910503.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1774 a         1644 c         1831 g         1788 t
ORIGIN      
        1 tgaattttaa tgatagcgct ttttgtatct tgttgatatt aatcaattaa tttgaattca
       61 tgatgaagtg atgattttat aaaacgtttt ttcatttttg cgagatggct ctcattatac
      121 gtgttctgtt aattaatgag cacagtgata attaatggcg aaatgaatga cgtacatttt
      181 ccacatcttt gagttgcggt tattacacca tttcaaaaac tcaacgccag gtaatgcggc
      241 ctattgactg gttaaaaaga agacatcccg catgggtacc aaagacaaca aggatttcca
      301 ggctaatctt atggattaat ctgctgtgca ttcgacaatt tgtctgacaa attggctttc
      361 ccttatgtct tttcccgcta aatttatgca cgttctcact gtaattctgc gatgtgatat
      421 tgctctccta tggagaatta atttctcgct aaaactatgt caacacggtc acttatcttt
      481 tagttaaaag gtaatgcttt gttttccgat taatttaacg aatgtcattc gtttttgccc
      541 tacacaaaac gacactaaag ctggagagaa ccatgcacaa atttactaaa gccctggcag
      601 ccattggtct ggcagccgtt atgtcacaat ccgctatggc ggagaacctg aagctcggtt
      661 ttctggtgaa gcaaccggaa gagccgtggt tccagaccga atggaagttt gccgataaag
      721 ccgggaagga tttagggttt gaggttatta agattgccgt gccggatggc gaaaaaacat
      781 tgaacgcgat cgacagcctg gctgccagtg gcgcaaaagg tttcgttatt tgtactccgg
      841 accccaaact cggctctgcc atcgtcgcga aagcgcgtgg ctacgatatg aaagtcattg
      901 ccgtggatga ccagtttgtt aacgccaaag gtaagccaat ggataccgtt ccgctggtga
      961 tgatggcggc gactaaaatt ggcgaacgtc agggccagga actgtataaa gagatgcaga
     1021 aacgtggctg ggatgtcaaa gaaagcgcgg tgatggcgat taccgccaac gaactggata
     1081 ccgcccgccg ccgtactacg ggatctatgg atgcgctgaa agcggccgga ttcccggaaa
     1141 aacaaattta tcaggtacct accaaatcta acgacatccc gggggcattt gacgctgcca
     1201 actcaatgct ggttcaacat ccggaagtta aacattggct gatcgtcggt atgaacgaca
     1261 gcaccgtgct gggcggcgta cgcgcgacgg aaggtcaggg ctttaaagcg gccgatatca
     1321 tcggcattgg cattaacggt gtggatgcgg tgagcgaact gtctaaagca caggcaaccg
     1381 gcttctacgg ttccctgctg ccaagcccgg acgtacatgg ctataaatcc agcgaaatgc
     1441 tttacaactg ggtagcaaaa gacgttgaac cgccaaaatt taccgaagtt accgacgtgg
     1501 tactgatcac gcgtgacaac tttaaagaag aactggagaa aaaaggttta ggcggtaagt
     1561 aatttgccgg aaaaattccc ctctgcatga tgcagagggg gtgtgaacga ccagtgattc
     1621 acggagacgt tatgcaacag tctaccccgt atctctcatt tcgcggcatc ggtaaaacgt
     1681 ttcccggcgt taaggcgctg acggatatta gttttgactg ctatgccggt caggttcatg
     1741 cgttgatggg tgaaaatggc gcaggaaaat caactctctt aaaaatcctc agcggcaact
     1801 atgcgccaac cacgggttct gtagtgatta atgggcagga aatgtccttt tccgacacga
     1861 ccgcagcact taacgcgggc gtggcgatta tttaccagga actgcatctc gtgccggaaa
     1921 tgaccgtcgc ggaaaacatc tatctcggcc agctgccgca taaaggcggc attgtgaatc
     1981 gctcattgct gaattatgag gcgggtttac aacttaaaca tcttggtatg gatattgacc
     2041 cggacacgcc gctgaaatat ctctccattg gtcagtggca gatggttgaa atcgccaaag
     2101 cgctggcgcg taacgccaaa attatcgcct ttgatgagcc aaccagctcc ctctctgccc
     2161 gtgaaatcga caatcttttc cgcgttattc gtgaactgcg aaaagagggg cgggtgatct
     2221 tatacgtttc tcaccgtatg gaagaaatat ttgccctcag cgatgccatt actgtcttta
     2281 aagatggacg ttatgtcaaa acctttaccg atatgcagca ggttgaccac gacgcgctgg
     2341 tgcaggcgat ggtcgggcgc gacattggcg atatctacgg ctggcaaccg cgtagttatg
     2401 gcgaggagcg cctacgtctt gatgctgtga aagcaccagg cgtgcgtacg ccaataagtc
     2461 tggcggttcg cagtggtgaa attgttgggc tgtttggtct ggtaggggcg gggcgtagcg
     2521 aattaatgaa aggcatgttt ggcgggacgc aaatcaccgc cggtcaggtt tatatcgacc
     2581 aacagccgat cgatattcgt aaaccgagcc acgccattgc cgcaggcatg atgctctgcc
     2641 cggaagatcg caaagcggaa ggcattattc ccgtgcactc cgttcgcgac aatatcaaca
     2701 tcagtgccag acgtaaacat gtgctcggcg gttgtgtaat caacaacggt tgggaagaaa
     2761 acaatgccga tcaccacatt cgttcgctca acatcaaaac gccgggcgcg gagcaactga
     2821 tcatgaatct ctcaggcgga aatcagcaaa aagccattct gggccgctgg ttatcggaag
     2881 agatgaaggt cattttgctg gatgaaccta cgcgcggcat tgatgttggc gctaagcacg
     2941 aaatatataa cgtaatttat gcgctggcgg cgcagggcgt ggcggtgctg tttgcctcca
     3001 gcgacttacc tgaagtcctc ggcgttgccg accggattgt ggtgatgcgg gaaggtgaaa
     3061 tcgccggtga attgttacac gagcaggcag atgagcgtca ggcactgagc cttgcgatgc
     3121 ctaaagtcag ccaggctgtt gcctgagtaa ggagagtatg atgtcttctg tttctacatc
     3181 ggggtctggc gcacctaagt cgtcattcag cttcgggcgt atctgggatc agtacggcat
     3241 gctggtggtg tttgcggtgc tctttatcgc ctgtgccatt tttgtcccaa attttgccac
     3301 cttcattaat atgaaagggt tgggcctggc aatttccatg tcggggatgg tggcttgtgg
     3361 catgttgttc tgcctcgctt ccggtgactt tgacctttct gtcgcctccg taattgcctg
     3421 tgcgggtgtc accacggcgg tggttattaa cctgactgaa agcctgtgga ttggcgtggc
     3481 agcggggttg ttgctgggcg ttctctgtgg cctggtcaat ggctttgtta ttgccaaact
     3541 gaaaataaat gctctgatca cgacattggc aacgatgcag attgttcgag gtctggcgta
     3601 catcatttca gacggtaaag cggtcggtat cgaagatgaa agcttctttg cccttggtta
     3661 cgccaactgg ttcggtctgc ctgcgccaat ctggctcacc gtcgcgtgtc tgattatctt
     3721 tggtttgctg ctgaataaaa ccacctttgg tcgtaacacc ctggcgattg gcgggaacga
     3781 agaggccgcg cgtctggcgg gtgtaccggt tgttcgcacc aaaattatta tctttgttct
     3841 ctcaggcctg gtatcagcga tagccggaat tattctggct tcacgtatga ccagtgggca
     3901 gccaatgacg tcgattggtt atgagctgat tgttatctcc gcctgcgttt taggtggcgt
     3961 ttctctgaaa ggtggcatcg gaaaaatctc atatgtggtg gcgggtatct taattttagg
     4021 caccgtggaa aacgccatga acctgcttaa tatttctcct ttcgcgcagt acgtggttcg
     4081 cggcttaatc ctgctggcag cggtgatctt cgaccgttac aagcaaaaag cgaaacgcac
     4141 tgtctgatgc ttttttctgc aacaatttag cgttttttcc caccatagcc aaccgccata
     4201 acggttggct gttcttcgtt gcaaatggcg acccccgtca cactgtctat acttacatgt
     4261 ctgtaaagcg cgttctgcgc aacacaataa gaaaagagaa ggaggagaac cgggtgacag
     4321 aaccgttaac cgaaacccct gaactatccg cgaaatatgc ctggtttttt gatctcgatg
     4381 gaacgctggc ggaaatcaaa ccgcatcccg atcaggtcgt cgtgcctgac aacattctgc
     4441 aaggactaca gctactggca accgcaagtg atggtgcatt ggcattgata tcagggcgct
     4501 caatggtgga gcttgacgca ctggcaaaac cttatcgctt cccgttagcg ggcgtgcatg
     4561 gggcggagcg ccgtgacatc aatggtaaaa cacatatcgt tcatctgccg gatgcgattg
     4621 cgcgtgatat tagcgtgcaa ctgcatacgg tcatcgccca gtatcccggt gcggagctgg
     4681 agacgaaagg gatggccttt gcgctgcatt atcgtcaggc tccgcagcat gaagacgcat
     4741 taatgacatt agcgcaacgt attactcaga tctggccaca aatggcgtta cagcagggaa
     4801 agtgtgttgt cgagatcaaa ccgagaggta ccagtaaagg tgaggcgatt gcagctttta
     4861 tgcaggaagc tccctttatc gggcgaacgc ccgtatttct gggcgatgat ttaaccgatg
     4921 aatctggctt cgcagccgtt aaccggctgg gcggaatgtc agtaaaaatt ggcacaggtg
     4981 caactcaggc atcatggcga ctggcgggtg tgccggatgt ctggagctgg cttgaaatga
     5041 taaccaccgc attacaacaa aaaagagaaa ataacaggag tgatgactat gagtcgttta
     5101 gtcgtagtat ctaaccggat tgcaccacca gacgagcacg ccgccagtgc cggtggcctt
     5161 gccgttggca tactgggggc actgaaagcc gcaggcggac tgtggtttgg ctggagtggc
     5221 gaaacaggga atgaggatca gccgctaaaa aaggtgaaaa aaggtaacat tacgtgggcc
     5281 tcttttaacc tcagcgaaca ggaccttgac gaatactaca accaattctc caatgccgtt
     5341 ctctggcccg cttttcatta tcggctcgat ctggtgcaat ttcagcgtcc tgcctgggac
     5401 ggctatctac gcgtaaatgc gttgctggca gataaattac tgccgctgtt gcaagacgat
     5461 gacattatct ggatccacga ttatcacctg ttgccatttg cgcatgaatt acgcaaacgg
     5521 ggagtgaata atcgcattgg tttctttctg catattcctt tcccgacacc ggaaatcttc
     5581 aacgcgctgc cgacatatga caccttgctt gaacagcttt gtgattatga tttgctgggt
     5641 ttccagacag aaaacgatcg tctggcgttc ctggattgtc tttctaacct gacccgcgtc
     5701 acgacacgta gcgcaaaaag ccatacagcc tggggcaaag catttcgaac agaagtctac
     5761 ccgatcggca ttgaaccgaa agaaatagcc aaacaggctg ccgggccact gccgccaaaa
     5821 ctggcgcaac ttaaagcgga actgaaaaac gtacaaaata tcttttctgt cgaacggctg
     5881 gattattcca aaggtttgcc agagcgtttt ctcgcctatg aagcgttgct ggaaaaatat
     5941 ccgcagcatc atggtaaaat tcgttatacc cagattgcac caacgtcgcg tggtgatgtg
     6001 caagcctatc aggatattcg tcatcagctc gaaaatgaag ctggacgaat taatggtaaa
     6061 tacgggcaat taggctggac gccgctttat tatttgaatc agcattttga ccgtaaatta
     6121 ctgatgaaaa tattccgcta ctctgacgtg ggcttagtga cgccactgcg tgacgggatg
     6181 aacctggtag caaaagagta tgttgctgct caggacccag ccaacccggg cgttcttgtt
     6241 ctttcgcaat ttgcgggagc ggcaaacgag ttaacgtcgg cgttaattgt taacccctac
     6301 gatcgtgacg aagttgcagc tgcgctggat cgtgcattga ctatgtcgct ggcggaacgt
     6361 atttcccgtc atgcagaaat gctggacgtt atcgtgaaaa acgatattaa ccactggcag
     6421 gagtgcttca ttagcgacct aaagcagata gttccgcgaa gcgcggaaag ccagcagcgc
     6481 gataaagttg ctacctttcc aaagcttgcg taggagcggt taatcacccg taagtggaac
     6541 taacagcaca tcgacctcac ttgaggcaat cactcttttc gccgagcagg atgctcgcga
     6601 aaagaaactg tgattgtgat taccgcaaat caccaaatcg aaatgatgtt tgtgacatac
     6661 ttccagaata tgttcgctta actcaccgta ggcaataaac gtcttgtcaa cgggataccc
     6721 cgcatcctga attaacttat caagaaagct ttgcgtttct tcttgcatca cgctgcgtaa
     6781 atcttcaagc atcggcgcgg ctaattgatt gtacatttcc gggtcggaag cgagagtaat
     6841 taaactgatg tgtcccttta ctggcctggc gatagatacc gcttttgcca gcagttgctg
     6901 actttccggt gtaaccgcaa cagcgacaag aatattgcta tagctcataa cctgttcctt
     6961 attctgtgaa cttcaggtga cattaaagct ttactcctaa tggagaaacg acgcaatccc
     7021 aactcggtca aacaggt
//
LOCUS       DBANUU010000194         7020 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000194 DBANUU010000000
VERSION     DBANUU010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.197"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(81..1217)
                     /locus_tag="ACIE68_004290"
     CDS             complement(81..1217)
                     /locus_tag="ACIE68_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFX5910504.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1220..1582)
                     /locus_tag="ACIE68_004291"
     CDS             complement(1220..1582)
                     /locus_tag="ACIE68_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFX5910505.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2119..4032
                     /gene="mtlA"
                     /locus_tag="ACIE68_004292"
     CDS             2119..4032
                     /gene="mtlA"
                     /locus_tag="ACIE68_004292"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFX5910506.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4262..5410
                     /gene="mtlD"
                     /locus_tag="ACIE68_004293"
     CDS             4262..5410
                     /gene="mtlD"
                     /locus_tag="ACIE68_004293"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFX5910507.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGTLPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5410..5997
                     /gene="mtlR"
                     /locus_tag="ACIE68_004294"
     CDS             5410..5997
                     /gene="mtlR"
                     /locus_tag="ACIE68_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFX5910508.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6009..6218)
                     /gene="yibT"
                     /locus_tag="ACIE68_004295"
     CDS             complement(6009..6218)
                     /gene="yibT"
                     /locus_tag="ACIE68_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFX5910509.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6503..6865
                     /locus_tag="ACIE68_004296"
     CDS             6503..6865
                     /locus_tag="ACIE68_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFX5910510.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1755 a         1779 c         1803 g         1683 t
ORIGIN      
        1 atcatcgcag ctaatgaagt aatatgcaaa aataattagt ccttacaagg agcaaagttc
       61 cataatctcc cccctccccc tcaatgatcc aaataaagat aatgcatcca gctggtcatt
      121 cttagcagca ctttccgcat caccgaaaca tggctgaaat ggtcggagta aaccgccacc
      181 tgggcgtaga tgccgtcggg taaggcatcg atatcatcgt tagggtccag ttcaatggtt
      241 cccagcacac cgtccgtgcc gggcacgacc gttaatgatt gcaatacccc ctgcgcctga
      301 taagaaccgc ctggcacgac aggtaaaata ctggtcagtt tgccgtggaa cacctgccca
      361 ggtagcgcgt taaacaccac ttctgcatca tcaccaggtt tcagacgtaa cagcgagttt
      421 tgccgaaatt gggcgacaat ttgccgtttt tgctcgggga tgaaaaccat caccggacgc
      481 aacggcaagg cagctgcgta tgtgcctggg cggatcagta cctgagtgac gtagccattg
      541 cttggtgcgc gaatgacagt ctgctcaaga ttatattttg cttcagtaag ttgcgctctt
      601 aagctcacaa tctgcgattg ctcgccgtta accatactgt cgagctggct ctggatctgc
      661 gcctgctccg ccaccgagcc tttcaccagc gcatcctgcg cgaggaaatt ttgccgcgca
      721 tcgtcgatgt cacgttccga gaacggattc accgccgcct ggctgccttt cagataacgt
      781 tgataatttt taaacagacg gtcgcgctcc gctgaaacct gggtggtgtt ggcctgcgct
      841 tctgtgagct gcgcgcgcag agtctttata ttatgcgtcg ccgtcatcag gtcagcctga
      901 agcctgtcaa ctcgcgcctg gtaacgaacc gggtcgagct taaaaagcac ctcgcccttt
      961 tgaataagct gattattctt gtcagtgact tcagtaacaa ttcccgtcac ctgtggcgtg
     1021 atggggatcg ctatcactgc cttttgcgcg gtaaaagtgt aagggtggtt gtagttcatc
     1081 aacaaaatca aaccactcac cagaaacacg ccacccagcg ccgccgtcgc cagcgtccac
     1141 tgattcaccg gaatacgaaa gattttaaag accgcccacg ccagcgccac gtaagttaaa
     1201 acaatcaata gatccataat tagatctccg ggaacgtgga atagtcagtc tttttctcgg
     1261 cggcgagttg atgctccagc cgggaaattc ggtcagaaag tgcggcgatt tccgggtcag
     1321 tcgctttctc ctgcgacgca acatgtgact gcatacccca gccacgctcc ggttgataga
     1381 gcgtcgccca gatccacaga aacggccaga taacatgcag agtaaacagg ctcacccagc
     1441 ccgccgtatg aatagcgtcg gcatggggat ggttgcgctt tttggcaatc agataaggga
     1501 tgtcatgtat cgcgatgatt ccataaaaaa tcaccagaaa gacgaagatc agcactccca
     1561 acgcgaaata gtttagaaac atatctgcct cggattcacg tttatcagtg ttgtttttgg
     1621 gctggcagcc agaagggagt caggctgata ttttgacaat aatccgggtt cgcgattctc
     1681 gccataacac caaagaataa tttttagagg tgatgagttg cttagttaca taacgattgt
     1741 atgacgaagg cataacatgc tgtagatcac atcaggtgaa cgccgtaaga aaatatcttg
     1801 tgattcagat cacaaagatt caacaaacca tcaaaacaaa aatgtgacac tactcacatt
     1861 taaatgccat ttttagcgaa aatcgccgcc ttgttgcttt tttacacaag cgttttgtga
     1921 tgaacgtcac gtcaattacc tctctacccc ctatatttat gtgattgata tcacacaaaa
     1981 ggccgtcgac tggacagtta accgattcag tgccagattt cgcagtatct acaaggtccg
     2041 gctacctctg ccgccacatt aacaaaaaac ctcgggcttc cagcctgcgc gacagcaaac
     2101 ataagaaggg gtgtttttat gtcatccgat attaagatca aagtgcaaag ctttggtcgt
     2161 ttcctcagca acatggtgat gccaaatatc ggcgcgttta tcgcgtgggg tatcatcacc
     2221 gcgttattta ttccaacagg gtggttaccg aacgagacgc tggcgaagct ggtcgggccg
     2281 atgatcactt atctcctgcc gctgctgatc ggttataccg gtggtaagct ggtaggcggc
     2341 gaacgtggcg gcgtagtcgg tgccatcacc accatgggcg ttatcgtcgg cgcagacatg
     2401 ccgatgttcc tcggttctat gattgcaggt ccgctgggcg gctggtgcat taagcacttc
     2461 gaccgctggg tagacggtaa gatcaaatcc ggttttgaga tgctggtgaa taacttctcc
     2521 gcaggcatca tcgggatgat cctcgctatt ctggcattcc tcggcattgg cccgattgtt
     2581 gaagccctgt ccaaaatgct ggctgcgggc gttaacttca tggttgtcca tgacatgctg
     2641 ccgctggcgt ctatctttgt tgaaccggcg aaaatcctgt tcctcaacaa cgccattaac
     2701 cacggtatct tctcgccgct gggtattcag cagtcccatg aactgggtaa atcaatcttc
     2761 ttcctgattg aagctaaccc aggtccaggt atgggcgtgc tgctggcgta catgttcttt
     2821 ggtcgtggta gcgctaaaca gtctgcgggc ggtgcggcaa tcatccactt cctggggggt
     2881 atccacgaaa tctacttccc gtatgtgctg atgaatccgc gtctgatcct cgcagtcatc
     2941 ctcggcggta tgactggcgt gttcacgctg actatcctgg gcggtggtct ggtttctccg
     3001 gcatctccgg gttctatcct tgctgtactg gcgatgacac caaaaggtgc ttatttcgct
     3061 aacatcgcgg gtgtgtgtgc ggcgatggct gtctccttcg ttgtctctgc tattttgctg
     3121 aaaaccagca aagtgaaaga agaagatgat attgaagcag caactcgtcg tatgcaggac
     3181 atgaaagctg agtctaaagg cacatctccg ctgtctgcgg gcgatgtgac taacgacctg
     3241 agccacgtac gtaaaatcat cgttgcctgt gacgccggta tgggttcaag tgcgatgggc
     3301 gcaggcgtgc tgcgtaagaa aattcaggat gcaggtctgt cacagatttc ggtcactaac
     3361 agcgcgatca acaacctgcc gccagatgtg gacctcgtca tcactcaccg tgacctgacc
     3421 gaacgcgcta tgcgccaggt tccgcaggcg cagcatattt cgctgaccaa cttcctcgac
     3481 agcggcctgt acaccagcct aaccgaacgt ctcgttgctg cccagcgcca tactgaaaac
     3541 gaagtgaaag tgaaagacag tctgaaagac agctttgacg attccagtgc taacctgttc
     3601 aaactgggcg cggagaacat cttcctcggt cgcaaagcag caaccaaaga agaagcgatt
     3661 cgttttgctg gcgagcagct ggtgaaaggc ggttacgttg agccggaata cgttcaggcg
     3721 atgctggatc gtgaaaaact gaccccgact tatctgggtg agtctatcgc ggtgccacac
     3781 ggtacggttg aagcgaaaga tcgcgtactg aaaacgggcg tggtgttctg ccagtacccg
     3841 gaaggcgtgc gcttcggtga agaagaagat gacattgccc gtctggtgat tggtattgca
     3901 gcccgtaaca acgagcacat tcaggttatc accagcctga ccaatgcact ggatgatgag
     3961 tccgtcatcg agcgtctggc acacaccacc agcgtggatg aagtgctgga actgctggca
     4021 ggtcgtaagt aatccaatcc caccctctcc acatggagaa ggtggggtta attgcctgat
     4081 gcgctacgct tatcaggcct acaggatgca tcacaatttg ttgaatttgc acgttcttgt
     4141 aggccggata aggcgcttac gccgcatccg gcgcggcccc tctcctcacg gagagggttt
     4201 gggtgaggga aaagcctcac cccagccctc tcgggtaaaa acattgatga aggttaatac
     4261 tatgaaagca ttacattttg gcgcaggtaa tatcggtcgt ggctttatcg gtaaactgct
     4321 ggcagacgcg ggtatccaac tgacgtttgc cgatgtcaat caggtggtac ttgatgccct
     4381 gaatgcccgt catagctatc aggttcatgt ggttggtgaa accgagcagg tagataccgt
     4441 ttccggcgtc aatgctgtca gcagcattgg tgatgatgtc gttgatctga ttgctcaggt
     4501 cgatttagtc actaccgccg ttggcccggt tgtgctggaa cgtattgctc cggctatcgc
     4561 caaagggctg gtgaaacgca aagaacaagg taatgaatcc ccgctgaaca tcatcgcctg
     4621 tgaaaacatg gtacgcggta ccacgcagct gaaaggccat gtgatgaacg ccctgccgga
     4681 agacgccaaa gcgtgggtag aagaacacgt tggctttgtc gattccgccg ttgaccgcat
     4741 cgtaccgcct tcggcttcgg caactaacga tccgctggaa gtgacggtag aaactttcag
     4801 cgaatggatt gtcgataaaa cgcagttcaa aggcacactg ccgaacatcc caggcatgga
     4861 gttaaccgac aacctgatgg catttgtcga acgtaaactc ttcaccctga acacgggtca
     4921 tgctataacc gcgtacctcg gaaaactggc tggtcatcag accattcgtg acgctattct
     4981 cgacgagaaa atccgcgcgg tggtaaaagg tgcgatggaa gaaagtggtg cggtactgat
     5041 caagcgctac ggctttgacg cagacaaaca tgcggcgtac atccagaaaa tcctcggtcg
     5101 ttttgagaac ccgtatctga aagatgatgt agagcgcgta ggccgtcagc cgctgcgtaa
     5161 actgagtgct ggcgaccgtc tgatcaagcc actgctcggt acgctggaat acagccttcc
     5221 gcacaagaat ctgattcagg ggattgctgg tgcaatgcac ttccgcagtg aagatgatcc
     5281 gcaggctcag gaactggcag cactgatcgc tgacaaaggt ccgcaggcgg cgctggcaca
     5341 gatttccggt cttgatgcca acagcgaggt tgtatccgag gcggtaaccg cttataaagc
     5401 aatgcaataa tggtggacca ggcgcaggac accctgcgcc cgaataacag attgtcagat
     5461 atgcaggcaa caatggaaca aacccaggcc tttgaaaacc gtgtgcttga gcgtctgaat
     5521 gctggcaaaa ccgtgcgaag ctttctgatc accgccgtcg agcttttgac cgaggcggta
     5581 aatcttctgg tgcttcaggt attccgcaaa gacgattacg cggtgaagta tgctgtagaa
     5641 ccgttactcg acggcgacgg tccgctgggc gatctttctg tgcgtttaaa actcatttac
     5701 gggttgggcg tcattaaccg ccaggaatac gaagatgcgg agctgctgat ggcattgcgt
     5761 gaagaactaa atcacgacgg caacgagtac gcctttaccg acgacgagat ccttggaccc
     5821 tttggtgaac tgcactgcgt ggcggcgtta ccaccgccgc cacagtttga accagcagac
     5881 tccagtttgt atgcaatgca aattcagcgc tatcaacagg ctgtgcgatc aacaatggtc
     5941 ctttcactga ctgagctgat ttccagaatc agcttaaaaa aagcctttca aaagtaagca
     6001 acgtctgctt actgcccctc tacctgcttc ggccgataaa gccgacgata atactccaga
     6061 cgttgtagat ataacggcac gctttcatcg ggtattccgg acggaatcgc gttacgggga
     6121 ggaagttttt tcagatactc ccggaacgcc tggcttgatg ccatgaaatc tacggcttta
     6181 tcgataagaa gcggaacgtt ttcacctaat ttgcccatga tgatatctcc gtattacccc
     6241 gcgccgggaa tgcgcggccg ccaattttag tttaggttcc cggtaaagat gacattaagg
     6301 aaaacgtgct gaatcctcaa taatcatccg ctgctgttta ttatcttttt tctatagatt
     6361 tcttaattaa tcaacgaatt gcgtagtttt tgagcatgtt aatgattacg ttgcagaact
     6421 attacaatgt catcagttgt gaacaaagca cctggtcgcg catactaggg gctataaatt
     6481 tatctttatc agaagccatc acatgaaaga agtcgaaaaa aacgaaatca aacgtctcag
     6541 cgatcgcctg gacgccatcc gccaccagca ggccgatcta tcgctggttg aagccgcaga
     6601 caaatatgcc gagctggaaa aagagaaagc cacgctggaa gcagaaattg ctcgcctgcg
     6661 tgaggttcat agccaaaaac tgagtaaaga agcacagaag ctgatgaaga tgccgtttca
     6721 gcgcgcaatt accaaaaaag agcaggctga tatgggcaag ctgaagaaaa gtgttcgcgg
     6781 actggtggtt gtgcacccaa tgaccgcgct gggccgtgaa atgggcctgc aggagatgac
     6841 tggattctct aagactgcgt tttaagcgcc tcagacgact ggttcgatca tatcaacccg
     6901 catctggctt gccttagctg gtcaatgcgg ggactctcct gtctactcgc ctaattacca
     6961 aacaattcac tccctttacg tcgatttacc gctggttgat attctgaaaa tgcattttta
//
LOCUS       DBANUU010000195         6963 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000195 DBANUU010000000
VERSION     DBANUU010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.198"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            6..242
                     /gene="rclB"
                     /locus_tag="ACIE68_004297"
     CDS             6..242
                     /gene="rclB"
                     /locus_tag="ACIE68_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFX5910511.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKTILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            254..805
                     /gene="rclC"
                     /locus_tag="ACIE68_004298"
     CDS             254..805
                     /gene="rclC"
                     /locus_tag="ACIE68_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFX5910512.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILK"
     gene            complement(1007..1135)
                     /locus_tag="ACIE68_004299"
                     /pseudo
     CDS             complement(1007..1135)
                     /locus_tag="ACIE68_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            1438..2289
                     /locus_tag="ACIE68_004300"
     CDS             1438..2289
                     /locus_tag="ACIE68_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFX5910513.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLINFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCEYADMPKEWTTTRGLY
                     ASFRYEGNWENYPDWVRNIYLIELPARGLARVNGSDIERFYYNEDFVEKDGNDVVCEI
                     FIPVRPV"
     gene            complement(2429..6685)
                     /gene="fdeC"
                     /locus_tag="ACIE68_004301"
     CDS             complement(2429..6685)
                     /gene="fdeC"
                     /locus_tag="ACIE68_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001635811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFX5910514.1"
                     /translation="MSHYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     AFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPMTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDAYDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNDDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLIAKDAHGNTISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKMTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGEHSITASVNNAQKTVTVKF
                     KADFSTGQATLEVDGSTPKVANDNDAFTLTATVKDQYGNLLPGAVVVFNLPRGVKPLA
                     DGNIMVNADKEGKAELKVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSDVTLTASSENLVLDPKGTAKTNEQGQA
                     VFTGSTTIAATYTLTAKVEQANGQVSTKTAESKFVADDKNAVLAASPERVDSLVADGK
                     TTATMTVTLMAGVNPVGGSMWVDIEAPEGVTEKDYQFLPSKADHFSGGKITRTFSTSK
                     PGVYTFTFNALTYGGYEMTPVKVTINAVAAETENGEEEMP"
BASE COUNT         1716 a         1772 c         1560 g         1915 t
ORIGIN      
        1 tttatatgtt taaaaaatcc gttttatttg caacactatt atctggcgtt atggcatttt
       61 ccaccaatgc agatgataaa acaattctga aacatatcag cgtctcgtca gtatcagcat
      121 caccgacagt tctggaggat gccattgctg atatagccag aaaatataat gcttcatcct
      181 ggaaagtcac atcgatgcga attgataata attcaaccgc aacagcagta ttgtataaat
      241 aaggatgttc acaatggaaa aatacctgca cctgttaagt cggggagata aaattggcct
      301 gacattgatt cgtctgagta ttgcaattgt ttttatgtgg attgggttat taaagtttgt
      361 cccttacgag gcagacagca ttacaccatt cgtcgcaaac agtccactaa tgtcgttctt
      421 ttatgaacac ccggaagact ataaacagta tctgactcac gaaggcgaat acaaaccaga
      481 agcaagggca tggcaatcgg ccaataatac ctatggtttt tccaacggtc ttggcgtcgt
      541 ggaggtgatt attgctctgc tggttttggc taatcctgtc aatcgctggt taggtttatt
      601 gggagggctg atggcattta ccacaccgtt ggtaacactc tcatttttaa tcaccacccc
      661 ggaggcatgg gtacccgcat tgggtgacgc tcatcatggt ttcccttatt tatccggtgc
      721 tggtcgcctg gtattgaaag atactctgat gctggcaggt gcagtcatga taatggcaga
      781 ttcggcgcgg gaaattctta aataacgcag taatgaatcc agttcaacgt taaaaactga
      841 atattgataa tccgaacact gtctgttgtt cactttttca ggcgtcaggc cccctttctg
      901 atgatgaagc ctgacgccat attaccggag atatgacaga gtgaagatca atatcactcc
      961 cctgttaaaa ggtttttgtc gtcttaggta aaatacatga ttaatatcaa tcatgtattt
     1021 ttacagcaag acaaccacga acaaattcag gattaaaatg ttttaccgac tccccaacat
     1081 aacccaaatc aagcgaacta atttgtgcca tttctttatc tgtcagtgag aaatcgattt
     1141 ttgatgggca tctgcaatac tctgaagcag actatacgga aaattccact gtcagtccct
     1201 ccattaggca tgaacaatga gtctacgtta aaacgtaacc tcaaagtagt atgtggattt
     1261 tgatatcact tatgcaaaaa attcattaat aatgtaggac tgaaacctct ctattttcgg
     1321 ggacaacgaa gcagacgcta ccagtgcttt tgccttcgcc cttgctattt ttgatacact
     1381 tagggcccag ggtataacga aaatgtgcga tatgacaaag aattaacgga gaatgagatg
     1441 atcaggcaga agattctaca gcagctcctg gagtggattg agtgcaatct tgagcaccct
     1501 atttcaatcg aagatatcgc acagaaatct ggctacagca gacgcaacat ccagcttctg
     1561 ttccgaaatt tcatgcatgt gcctttggga gaatacattc gcaaacgaag gctttgtcgt
     1621 gccgccattc ttgtccggct caccgcgaaa tctatgcttg atattgcact ctctttgcat
     1681 tttgattcac agcagtcatt cagccgcgaa tttaaaaagt tattcggctg ctctccccgt
     1741 gaataccgcc accgtgatta ttgggatctc gcaaatatct tcccttcttt tttaatacgt
     1801 caacagcaaa agacggagtg tagattaatc aactttcctg agacacctat ttttggcaac
     1861 tcatttaaat atgacattga agtgtcgaat aaatcaccgg atgaagaagt caaactacga
     1921 cgtcatcatt tagccagatg tatgaagaat tttaagacgg atatctattt cgtttccacg
     1981 tttgaaccgt caacaaaatc ggtcgatttg ctcacggttg aaacttttgc tggtacggta
     2041 tgtgaatatg ctgacatgcc aaaagagtgg acaacgaccc gaggacttta tgcctctttc
     2101 cgttatgaag gaaactggga aaattatcct gactgggtgc gtaacatcta tctaatagag
     2161 ttacctgcca gggggttagc cagagtgaac ggcagcgata ttgagcgctt ttattacaat
     2221 gaagatttcg tagaaaagga tggcaatgat gttgtttgcg aaatttttat tcccgttcgt
     2281 ccggtttagt tggtcactat ctcttattga gttttatcct tgcctgatac tatttaatca
     2341 gtatcaggca aggtattcag tgaataatgg cgttgaatat ttcaacacca ttattcttta
     2401 ttagatcgta actttcatac tattcaaatt atggcatctc ctcctcgcca ttttcagtct
     2461 ctgcagcaac ggcgttaatt gtcaccttca caggcgtcat ttcgtacccg ccatacgtca
     2521 gtgcgttgaa tgtgaacgta tagacacctg gcttgctggt actaaatgta cgcgtgattt
     2581 tcccacctga gaaatggtca gccttcgacg gcaggaattg ataatccttc tccgtcactc
     2641 cttccggagc ctcaatgtcg acccacatac ttcctcctac gggattgact cccgccatca
     2701 gggtaaccgt cattgttgca gtagtcttcc cgtccgccac cagagaatct acacgttctg
     2761 gagatgcggc gagcaccgcg tttttatcat ccgcgacgaa tttagattca gcagttttcg
     2821 tcgatacctg accgttggcc tgttccactt tcgccgtgag tgtatatgtc gctgcgatag
     2881 tggtagagcc ggtgaaaaca gcctgtcctt gctcattagt tttcgccgtc cctttaggat
     2941 ccagaactaa attttccgag ctggcagtca gcgtcacgtc gctatccttc aacaggttgt
     3001 tattggcatc agtcaccgta actttatacg tctgtttggt tttgccatcc gccactgcac
     3061 ggttgccaat cacctcaata ctggagatgg tcgccgtagt cttatcggcc acaaacgtta
     3121 cagactgcgc attcgaaggc tggtcatttc ctgccgacgc cgtgatctca tacgttccgg
     3181 cagtcacgga gaccactttc agttccgctt taccctcctt gtcggcgttc accatgatat
     3241 taccgtctgc aagcggtttg acgccccgag gcagattaaa gacgaccaca gcgccaggca
     3301 gaaggttgcc gtattgatcc ttaaccgttg ccgtcagcgt aaaggcatca ttgtcgtttg
     3361 ccacttttgg cgtgctgcca tcaacctcca gggtcgcctg accggtactg aaatccgcct
     3421 tgaatttcac cgtaaccgtc ttctgagcat tattcactga ggccgtgatg ctgtgttccc
     3481 ccgcaaccgt tgacataagc tcaatgtcca ctttccctgc cgcattggtt gttactgtat
     3541 tccccgtctt gctggtcaca ccctgcggta aagtcagcgt aacttcttgc ccctgcaacg
     3601 ggttaccata gctgtccacg acggtcaggg ttatctggtt agcagactgt ccattagcca
     3661 gttggttatt aaccttcact gtcatatcac gaatctcagc cttagatgcg tcacctgcaa
     3721 cgttcagcac cagtggctga gcaacggcat tttggccgtt cactcgcggc atcacagaca
     3781 actgacccgc ggcagatccc agcgttaagg tcgccacgta gaccccatta cctttctctg
     3841 tccagtttcc tgctgaagga cgctcactcc ccgtgctggc ggcaccgcta aacactggtg
     3901 catctggctt cagcccggtg acagggttcc cgtacgcatc cttcacggtg aaggtgagtt
     3961 ccgtcgtcat tttgacggtc ggagccttat tgtccgcaac aagcgtagag ttcgctgatg
     4021 acatctctcc ggcgatgacc gtcaactgcg cggcttcggt ggctgctggc tggccgttga
     4081 agagcggcat gacacgaagc tcgcccgtct ttccgcctgt agttaacgta gcaacatagg
     4141 aaccgtcacc tttttcggtc caactggaaa cggtcgcccc ttcagaggcg gtccccgtca
     4201 aacttgccga caacgaaaga ccactgatag tgttgccatg tgcatctttt gcaataagcg
     4261 tcacggttgt gctttcgccg gcttttacgt gatcttctgc gacagagact ttcgactggt
     4321 ttgaagataa cgcatcagcc accacggtta cttttgccgc atttgctgcc gcatcctgtc
     4381 catttagctt cggcataact tctaattcac ccgccgtaga gccgagagta atctgcgcag
     4441 tccacgtccc atcatcatta tttgtccagc cagatgccgt agaacctaca gcagcggcac
     4501 ccgctaatga cggcgcttcc ggcgtgaggc tggtcacagg gttgtcatag gcatcttttg
     4561 ccgtccagat tgccgtaact gtaccgccaa ccaccggttt atcaggattc agggtgatgg
     4621 acgaatgtgc tgcatcaagc ggaccggcaa caaacttcag cgtttgttgt aatgtgccca
     4681 gctgatactg ctcgctgaaa gcacgcacaa caacgtttcc ggcacgggtc gaagaaaccg
     4741 tggcgctgta aacgcctggt tttatttccg aaatggcacc aacggttaca ccattagtgt
     4801 cttgcggaac aaatcgcaag cggctggctt ctcccgtcat cggattaccc tcggagtcca
     4861 ccgcagtcaa cgtcaacgta taggcttgct gaccatcagc aaccacatca cctgacggct
     4921 cgttagcgct cagggtggag tttgccacat ccatcatcgt ggcccgcagt tctgcagtga
     4981 cggttttgct catgccatca acgctaacag taatcgttgc ctcacctgac tgcgttccgg
     5041 tagtaaagac agactgatac acccctgctt cagtttcggt gaactcaccc agtgttggct
     5101 ttgcctgtga tttagtggcc ttcagggaac gagtcacaat atttccagcc ggtttgaagg
     5161 ctagttcagt cttgatctga tctttcatgc ccgtgactgg ctgcccctcg gcgtcgcgca
     5221 gagacagcac cagcggtctt tgctcattac cgttagcaag catttgaata cggctctgac
     5281 cgtcaagcgt taacgccgtg cgatcggcgc tcatacctgc tccggtaatg accacctctg
     5341 tctgcacgcg ttttgaggca ttgcctttgt tatcgtaggc aaccgcagaa atcgcataat
     5401 aattgtcttt gcctggacga taagccggga gcgttacttg ccactgacta ccctgaccgg
     5461 taattttgcc accctcagcc agtaatgacg gcgcttccca ctgcacattt ttcagtccgt
     5521 gagttgcttt gctgaccaca agccccaggg aaagtgtctg accaccctta ccttcaatac
     5581 gttcaggcag agcaatacgg atcacttcag atttgcggta ctcaagaacg atgttgttat
     5641 tacgctcaac caggtcatag cggctgcctg ccagtacccg acgctcgcga atgctgtctg
     5701 tatcgagttg tttcgccaga ggttcgccaa tccgataatt aacttccagg ccaaagcgag
     5761 tgtcattctc accactcttg ccctgcttat gcccggcgct cagtgtcaga agaggcactg
     5821 gcgtataggt cacctcggca gaaatagcat gcgggtcttt ctggcgctta tctttaccaa
     5881 acagcccgac ttcatcgcca taatactgtt catacatcag gcttgcgcca agctgcggcc
     5941 aggcgggtaa atagccctca gcacgaatat cccagccatt cgccgggcgt tcctgataat
     6001 cctcaacatc cggcgatttt ttccagccag aagcccggat ataaccattg gcgctcagtt
     6061 tcagataatc gcgccagtat tccgcaccaa caccaatgcg ggtatgacta cgggataaat
     6121 catggtcgat aaaggtgttc acccccgcca tccagtcatt tcctgaaaaa tgacgccagc
     6181 caaaaccaat atttgactga gtacgatcgt cggtacgatg tattgctccc tgagtgaaca
     6241 acatatttgt tggcgtatca taaatcggat aaagcatttc cagcgaagaa tccttcagcg
     6301 agaaatcttt atcgacattc agtttgacgc gcgcagtacc atatttcccg agccactcct
     6361 gtatttcctg gttagcttta gctgtggcca ttccggtaat aaagttacgt gtcgcatcgc
     6421 tatctggctg actgcttaaa aatgtcccgg catttgcggc aaacgacgcg acatttttct
     6481 ccacgttatt atcagcagtt accgtagtat ttcccatgct caaccgtggc tgaaccgcat
     6541 gctgcgcacg tgccgccatt actggggtaa aggtgacagc gagtggaaaa agaacctgaa
     6601 cagagatatt tgcccacgcc acgcagcggg ccagaactga ataacgaaat cgtggttgtt
     6661 tatgacctgt tttataatgt gacattgaga taatttcctg atgaacgaat taacgaacat
     6721 aggcagacac aaataatggc gtctgcttgt cagaaaaacc ggggatttcc cgatttcctt
     6781 ttccgcagaa aaggaaatac ctttatgtaa gaaagtgcac cgccacctaa tatatccagt
     6841 gcctgtagcc actcatcagg tggttgggcg cgggattata tttttattta tgggggaaaa
     6901 tttagctttt tgtgcaagtc attaaataga tacctaagtt atatttctta ctctgcggac
     6961 agt
//
LOCUS       DBANUU010000196         6888 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000196 DBANUU010000000
VERSION     DBANUU010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.199"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            117..554
                     /locus_tag="ACIE68_004302"
     CDS             117..554
                     /locus_tag="ACIE68_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFX5910515.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGR
                     REFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDK
                     QAQDTERRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
     gene            635..1054
                     /locus_tag="ACIE68_004303"
     CDS             635..1054
                     /locus_tag="ACIE68_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFX5910516.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGF
                     DPNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGT
                     TQFAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
     gene            complement(1617..1850)
                     /locus_tag="ACIE68_004304"
     CDS             complement(1617..1850)
                     /locus_tag="ACIE68_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFX5910517.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMKLSQQVQHLSERTERLSQLYSENRR"
     gene            complement(1857..2375)
                     /locus_tag="ACIE68_004305"
     CDS             complement(1857..2375)
                     /locus_tag="ACIE68_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFX5910518.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SCGTLWYLGGMIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVI
                     PHQGKDWVQLME"
     gene            complement(2413..3111)
                     /locus_tag="ACIE68_004306"
                     /pseudo
     CDS             complement(2413..3111)
                     /locus_tag="ACIE68_004306"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3053..3376)
                     /locus_tag="ACIE68_004307"
     CDS             complement(3053..3376)
                     /locus_tag="ACIE68_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFX5910519.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     ARDDS"
     gene            3441..3632
                     /locus_tag="ACIE68_004308"
     CDS             3441..3632
                     /locus_tag="ACIE68_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFX5910520.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDSENL"
     gene            complement(4657..4794)
                     /locus_tag="ACIE68_004309"
     CDS             complement(4657..4794)
                     /locus_tag="ACIE68_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFX5910521.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISV
                     Q"
     gene            4842..5183
                     /locus_tag="ACIE68_004310"
     CDS             4842..5183
                     /locus_tag="ACIE68_004310"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="HFX5910522.1"
                     /translation="MSLRYYIKNILFGLYCALIYIYLITKNNEGYYFLASDKMLYAIV
                     ISTILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFG
                     LLGLFISIKNN"
     gene            complement(5180..6745)
                     /locus_tag="ACIE68_004311"
     CDS             complement(5180..6745)
                     /locus_tag="ACIE68_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="HFX5910523.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSK
                     SESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEAL
                     RHNSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIE
                     KEQAETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRL
                     SSSIHARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLR
                     ARAGDEMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKT
                     AQTNLLNSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAF
                     EKYKDVLNKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDI
                     LKIKDTGDWKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFID
                     KNKLNTINEVLGI"
BASE COUNT         1741 a         1889 c         1458 g         1800 t
ORIGIN      
        1 aaaaaaacag ccacccacac cacgtgctat ttccacccga tgccacaaaa accagcacaa
       61 acattaccgt tctcagacct cattatgttt tactgaaacc atgagatgag acacctatgg
      121 gacactgtaa ctttatggca tggcacacac tccgggacgc actgaaaatg actggaagat
      181 cgcgttcaca gttttaccgt gatatgcgct caggtcttgt cagttaccgt accagccagg
      241 acggacgacg ggagtttgaa accagtgaac tgatccgggc atacggcgaa ttaaagcgga
      301 atgagacacc agaaaggcac actcaaggac actcagaaaa acctcatgat cagcagacag
      361 aacgcattct ccgggaactg aatgagctga aacaatgcct gacgctgatg cttgaggata
      421 aacaagccca ggatacggag cgccggcgcc aggaagcaga acggaaacag ctacagaatg
      481 agatagccca gctcaggcag gctctggagc aggaaaagaa acggggattc tggtccaggc
      541 tgttcggtca ctgaacgcgc tcagaaactg atgataagat aatcttcgga taataactca
      601 ccgagaataa atactttaag gtagggagac actcatgaga cgtaccggaa acaaactttg
      661 tcttatcgcc atgataacag caacagtagc tctcacagcc tgtaccccaa agggcagcgt
      721 ggaacaacat acccggcatt acgtatatgc ttctgatgac ggttttgatc ccaacttttc
      781 cacccaaaaa gccgacacaa cacgaatgat ggtgcctttt tttcggcagt tctgggatat
      841 gggagctaaa gataaagcga caggaaaatc acggagtgat gtgcaacaac gcattcaaca
      901 gtttcacagc caggaatttt taaactcact ccggggcaca actcaatttg cgggtactga
      961 ttaccgcagc aaagacctta ccccgaaaaa atccaggctg ctggctgaca cgatttctgc
     1021 ggtttatctc gatggctacg agggcagaca gtaagtggat ttaccataat cccttaattg
     1081 tacgcaccgc cgaaacgcgt tcggtgcgat cacggcagca gacaggtaaa aatggcaaca
     1141 aaccacccgg aaaactgccg cgatcgcacc cggtaaattt taaccacatg catacctatg
     1201 cagccaggtg aatcacgcag gaattgccag tcagagaccg ctgaaacgga tttttttcat
     1261 actttcggaa gcgaagaaga cggggatttg agcaggaaaa cagaagttca ggggaatgag
     1321 cgcgatctgg caatagaggc aaaaacacga ctatgaaaga cacaccagaa tcgcgcccgt
     1381 atgcgtttta acgcgttcct gtgttttcag caaggtttat gactgaagcg cgttcccggt
     1441 gccccgaacc aaccattatc ccaaaaaatg caccggaaac gcgctgacaa cattttagcc
     1501 tgtctctgat taccatccca gcgaagggcc atccagtgtc cgttcctgta tttccagctg
     1561 gcgctcacgc tccagggata actcatgttc gcgttcctgt atcagccgtt cgtcccttat
     1621 ctcctgttct cgctgtataa ctggctcaag cgttctgtcc gctcgctcaa gtgctgcacc
     1681 tgctgactca acttcatgac ccgctcgttc agcatcgcgt tgtcccgttg cgtaagcgaa
     1741 aacattttct gcaattccac gaaggcgctc tcccattcgt tcagccgctg catatagtcc
     1801 tgttgcagtt gctctaatgc gttcagcaaa tgtctttcca gctccgtcac tctgtgtcac
     1861 tccatcagtt gtacccagtc cttcccctga tggggaatca cctctgctga tttcccgtac
     1921 ggaaggacca gaaatttcct gttcccgtcc tgtacgaact ccacgcccca tgtcttcgcg
     1981 ttcagtttct gcaatgtctc ttcctgcttc ctgatatctt ccaggttcgc ctgtatcatc
     2041 cctccaagat accagagcgt cccgcaactc acggtaaaca ggaaaatgac catccccagt
     2101 aacatcatgc ccgtattccc tgccagcttt aacacgtcct tcctgtgctg catcatcgcc
     2161 tctttcaccc cttcccggtg ctttttcagt gattcctctg tcgaagctgt gaacagggct
     2221 atagcgtctt tgattttcgt ctcgtttgat gtcacagcct tgcttacaga tttttcgagc
     2281 ttgctgaact cgtcgttcag cattgtctct gtagattcgg ctctctcttt cagctttttc
     2341 tcgaactccg cgcccgtctg taaaagattg ctcatagaat gctcctttca gtctgatatt
     2401 cttcccgccg ttcgggtctg caatgctgat actgcttcgc gtcaccctga ccacttcaat
     2461 ccctgcttcc gtgagcgcct gaatcacatc ctgacggcct tttatctctc ctgcatggta
     2521 aagagcatct atcccgcgtg tgacgccctc agcaaacgcc tgtttcgttt ccggcaggtt
     2581 atcagggaga gtcagcgtcc ggcggttctc cggggcgttc gggtcatgca gcccgtaatg
     2641 gtgattcacc agcgtctgcc aggcatcaat tctcggcctg tctgcccggt cgtagtacgg
     2701 ctgtaggcgt tttccggtct ccagctccat gttcggaatg acaaaattca gctcaagccg
     2761 tcccttgtcc tggtgctcca cccacaggat gctgtactga tttttttcga gaccgggcat
     2821 cagtacacgc tcaaagctcg ccatcacttt ttcacgtcct cccggcggca gctccttctc
     2881 cgcgaacgac agaacaccgg acgtgtattt cttcgcaaat ggcgtggcat cgatgagttc
     2941 ccggacttct tccggattac cctgaagcac cgttgcccct tcccggttac gctcccttcc
     3001 cagcaggtaa tcaaccggac cactgccacc accttttccc ctggcatgaa acttaactat
     3061 catcccgcgc cccctgttcc ctgacggcca gacgcaaccg gcgcagctca tccccgatgg
     3121 ccatcagtgc ggccaccacc tgaacccggt cgccggaaga ccactgtccg ctgttcacct
     3181 tacgggctgt ctggttcagg ttattcccga tggctgccag ctgacgcagt aacggcggtg
     3241 ccagtgtcgg cagcctcccg gaacgggcaa ccggctcacc caggcagacc cgccgcatcc
     3301 ataccgccag ttgtttaccc tcacagcgtt ccagtaaccg ggcatgttca tcatcagtaa
     3361 cccgtatcgt gagcatcctc tcgcgtttca tcggtatcat tacccccatg aacagaaatc
     3421 ccccttacac ggaggcatca gtgactaaac aggaaaaaac cgcccttaac atggcccgct
     3481 ttatcagaag ccagacatta acgctgctgg agaagctcaa cgaactggac gcggatgaac
     3541 aggcagatat ctgtgaatcg cttcacgacc acgccgatga gctttaccgc agctgcctcg
     3601 cgcgcttcgg ggatgacagt gaaaacctct gacatatgca gctcccggag acggtcacag
     3661 cttgcctgtg agcggatgcc gggagcagac aagcccgcca gggcgcgtca gggtgtttta
     3721 gcgggtttcg gggcgcagcc ctgaaccagt cacgtagcga tagcggagtg tatactggct
     3781 taactatgca gcatcagtgc ggattgtatg aaaagtgcac catgaccagt gtgaaatacc
     3841 gcacagatgc gtaaggagaa aatgcacgtt ccggcgcttt tccgcttcct cgctcactga
     3901 ctcgctacgc tcggtcgttc ggctgcggcg agcggtactg actcactcaa aaacgataat
     3961 acagttatcc acagaatccg gggataagac cggaaagaac atgtgcgcaa aaaggcaaag
     4021 cccaacaaaa ggccacgccg atggcgtttt tccataggct ccgcccccct gacgagcatc
     4081 acaaaaaccg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     4141 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     4201 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgttggt
     4261 atctcggctc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     4321 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     4381 acttagcgcc actggcagca gccgttggta acttggttag cagagcgatg atggcgcaaa
     4441 tggtgcaaca gagttcttga agtagtggcc cgactacggc tacactggaa ggacagtatt
     4501 tggtatctgc gctctgctga agccagttac ctcggttaag cagttcccca actgacttaa
     4561 ccttcgatca aaccgcctcc ccaggcggtt ttttcgttta caggcagcag attacgcgca
     4621 gaaaaaaagg atctcaagaa gatcctttga tcttttctac tgaacagaga tccccgtcaa
     4681 tttagaagaa gatgatggag ccacagtccc tccctgaaca tcacgtatat agttagcctg
     4741 acatccaaca aggaggttta tagcgaatat tcccacaaaa aatcttttcc tcataactcg
     4801 atccttataa aatgaaaaga atatataacg aggtttaatt tatgagttta agatactaca
     4861 taaaaaatat tttgtttggc ctatactgcg cacttatata tatatacctt ataacaaaaa
     4921 acaacgaagg gtattatttc ctagcgtcag ataagatgct atacgcaata gtgataagca
     4981 ctattctatg cccatattca aaatatgcta ttgaacacat attttttaag ttcataaaga
     5041 aagatttttt cagaaaaaga aaaaacctaa ataatgcccc cgtggcaaaa ttaaacctat
     5101 ttatgctata caatctactt tgtttggtcc tagcaatccc atttggattg ctaggactgt
     5161 ttatatcaat aaagaataat taaataccca acacctcatt tatagtatta agtttattct
     5221 tatcaataaa ggcacataga atgcctgtaa caatagcaat cccccagata cctaatgtag
     5281 ttccagcaag cacactaaaa agtaaaacaa caacataact aactccagca tctacagctt
     5341 tcttctctaa tgtaagaaat agcggcttcc agtcacctgt atccttaatt tttaggatat
     5401 cagataccac atcatatcca aaagaaacat gtcccgtaat cttcaagtac ttggcaaact
     5461 gatctaaatg cttagcccag tcttcatact taaccgcctc cagtgcattg aaaattgcat
     5521 cacggtctgc tttgctgaat ttcttattta atacatcctt gtatttctca aaagcagcta
     5581 gagcttcatt cacattgctg attttcttac ctttagattt atcagcaagt tcctgtgcca
     5641 tttttgaata tttttcacca tatttttcag atagcgtttg ataaaagcta actgttgcat
     5701 ccacagcatc cttaatctgc gagttcagga gattagtctg tgctgttttc aaattatctt
     5761 cagcatcatg aatacgtgaa actgctgtac ttcgcttatt attagcctga gaaatagcct
     5821 cttggattcc atttatctca gagctaatct ggttaagacg cgtttctgat gctgttacct
     5881 gcttttgttt ttcctccatc tcatcaccag ctctcgcccg tagtctggtg gcctcaaaaa
     5941 aaggacgact ctgaagcgga tcattcgccc taggagataa aagtttaacc ctttcatcca
     6001 gttctttata tttagcagat gcctgatcca actcatttcg ttttccggac agcgtattcg
     6061 tttctgcatc acgggcatga atactggagc ttaaacgggt attgagcgta ttaatctctt
     6121 catccacttt cgccagctca gattgtgcag cagcaagatt tttttgtgcc acctccacag
     6181 cccgggcctc ttcactcaat gctgccaggc gtttctcttc atcctcagcc agtttcaatt
     6241 ggcgttctgt ttcagcctgc tccttctcta tctctttacg tcgttgttct gcttcctgaa
     6301 aagccttttc cgcagcttcc gcttctttac gggctttttc ttctgctttt gcaaggcgca
     6361 accgctctgc ttctgcctgc atcgctgcat tattggcatg agccaggtca ataacctccg
     6421 gatgagtgga attatggcga agcgcctcat tcacaatatc cttcagatgt tgagtcagcg
     6481 catcccggtt tgcttttgct ttagcctgtg cttctgcggc agcttttgct cgggcagcct
     6541 gttctgcctg cgttttcttc aattgagcag tagaccattt agctgtggca tgaatggctg
     6601 cagaactttc acttttgcta cctccagttc caccaccacc gccagagcca ctcccgtctg
     6661 gattaccatt caaaagagta atgattacct gccccttatc atcataagga acaccatctt
     6721 tatagtacgc tacagctgtt tccattataa aatcctcttt gacattaaaa acaaatagtt
     6781 aaaagcaata tactgtatat ataatcactg gttttatata cagtttaaaa ttacgccgct
     6841 gcattttccc tgtcaaccct gtggattttc atttttgtga aaacgatc
//
LOCUS       DBANUU010000197         6849 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000197 DBANUU010000000
VERSION     DBANUU010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.200"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..427
                     /locus_tag="ACIE68_004312"
     CDS             <1..427
                     /locus_tag="ACIE68_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFX5910524.1"
                     /translation="FDESIAFTCLSAFSSEKQFGVFLFGCLKSTGAKVKTIIHTDLSA
                     PWRLKDISSRLYLSESLLKMKLKEEGVSFSKIILDERMQMAEYLLSTRCYPISKVAKV
                     CGYASVSYFTYVFRRYFGVSPSQYSQRSSESKILTHQGI"
     gene            557..703
                     /locus_tag="ACIE68_004313"
                     /pseudo
     CDS             557..703
                     /locus_tag="ACIE68_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(703..786)
                     /locus_tag="ACIE68_004314"
                     /pseudo
     CDS             complement(703..786)
                     /locus_tag="ACIE68_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1099..1575)
                     /gene="ybhB"
                     /locus_tag="ACIE68_004315"
     CDS             complement(1099..1575)
                     /gene="ybhB"
                     /locus_tag="ACIE68_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFX5910525.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1634..2923)
                     /gene="bioA"
                     /locus_tag="ACIE68_004316"
     CDS             complement(1634..2923)
                     /gene="bioA"
                     /locus_tag="ACIE68_004316"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFX5910526.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGKR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3010..4050
                     /gene="bioB"
                     /locus_tag="ACIE68_004317"
     CDS             3010..4050
                     /gene="bioB"
                     /locus_tag="ACIE68_004317"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFX5910527.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4047..5201
                     /gene="bioF"
                     /locus_tag="ACIE68_004318"
     CDS             4047..5201
                     /gene="bioF"
                     /locus_tag="ACIE68_004318"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFX5910528.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIAADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQMVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5188..5943
                     /gene="bioC"
                     /locus_tag="ACIE68_004319"
     CDS             5188..5943
                     /gene="bioC"
                     /locus_tag="ACIE68_004319"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFX5910529.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPKGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            5936..6613
                     /gene="bioD"
                     /locus_tag="ACIE68_004320"
     CDS             5936..6613
                     /gene="bioD"
                     /locus_tag="ACIE68_004320"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFX5910530.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         1661 a         1757 c         1847 g         1584 t
ORIGIN      
        1 ttttgatgag tctattgctt ttacatgtct ctctgcattt tcttctgaaa aacaatttgg
       61 agtatttctg tttggatgtt tgaaaagcac aggggctaaa gttaaaacga ttattcatac
      121 ggatttatct gcaccatggc gtcttaagga tatatcatca agattatatc tcagcgaaag
      181 tttactaaag atgaaattga aagaagaggg ggtatcattc agtaagatca tacttgatga
      241 gaggatgcaa atggctgaat atttactcag cactcgttgt tatcctatta gtaaagtagc
      301 taaggtctgt ggttatgcca gtgtctcata ctttacttat gtatttagac gttattttgg
      361 tgtttctcca agtcaatact ctcagaggag ttcagaaagt aaaattctta ctcaccaggg
      421 aatctgatca ttgttcttgc ccccttattt ccagacaggg ggtgtatctt aagttaacgt
      481 tacccgctga cgttgaatcg ccacgggttt aacagacacc tcagagtcat ttaagatggc
      541 ttaaagagag gtgcccatga gcggtaagcg ttatcccgaa gagtttaaaa ctgaagcagt
      601 caaacaggtt gttgatcgcg gttattctgt tgccagcgtt gcaacacgtc tcgatatcac
      661 cacccacagc ctttatgcct ggataaagaa gtacggtccg gattactcca ctttttcatc
      721 aagccaatcc gcccaccact gcatcatttc tctgcgctta tcgagatact gagcatggtt
      781 gtaaattcaa ctaacccagt gcgttgacaa aaaattagcg caagaggaca aaaatcacct
      841 tgcgctaatg ctctgttaca ggtcactaat actatctaag tatttgattc atagtgactg
      901 gatatgttgt gttttacagc attatgcagt ctatttttta agccaaatgc attataagac
      961 attgatatta atgactttac tgtttcttgt tcagcttttt tatactaact tgagcgaaac
     1021 gggaaggtaa aaagacaaaa agttgttttt aataccttta agtgatacca gatggcattg
     1081 cgccatctgg cagagtgatt aactaaacat cgcagtaatc gaggcgcttg ccagagagtg
     1141 gaaatgaacg ttaaacccga ccatcgcgcc gctggcacct tcatcgacat caatacgttc
     1201 tatatccagc gcgtgaacgg taaaaatgta gcgatgagtt tcgcctttcg gcggtgctgc
     1261 gccatcgtac ccggttttac caaagtcggt acgcgtctgc aaaacgccgt ctggcattgc
     1321 taccagacca gagccaaacc cttgcggtaa tacgcgggta tcagcgggta agttaacaac
     1381 tacccagtgc caccagccgg agccggttgg cgcatccggg tcgtagcagg tgacaacaaa
     1441 acttttcgtt cccgcaggaa catcatccca cgccagatgc ggtgaaatat tatcgccatc
     1501 gtaacccatg ccgttaaaga catgacgatg cggcaattta tcgccatcgc gcagatcgtt
     1561 actgatgagt ttcatgaacc ctcctttctt gtttgcagaa agtgtagcca gaaaccctca
     1621 cgcggacttc ccgttattgg caaaaaaatg tttcatcctg taccgcgcgg ttaaccgctg
     1681 cggtcagacg ctgcaactgt tgcgggagaa taatataggg cggcatcagg taaatcagtt
     1741 tgccaaaagg ccggatccag acaccctgtt cgacaaagaa tttttgcagc gccgccatat
     1801 tcaccggacg agtggtttcg accacaccga ttgcccccag tacgcgcaca tcggcaacca
     1861 tttcggcatc acgggctggt gccagttgct cgcgcagctg cacttcaata gccgccacct
     1921 gctgctgcca ttcgccggat tcgataatcg ccaggctggc gtttgctgcc gcgcaggcca
     1981 gcggattgcc cataaaagtt ggcccatgca taaagcagcc ggcttcgccg ttactgatgg
     2041 tttctgcaac ctcgcgcgtg gtaagtgtgg cggaaagggt cattgtgccg ccggttaagg
     2101 ctttaccgag gcacaaaatg tccggcgcga tttctgcata ttcacaggca aacagtttgc
     2161 cggtacgacc aaatccggtg gcgatctcgt cggcaatcag caagatacct tcgcgatcgc
     2221 acattttgcg gattcgtttt aaccattccg gatggtacat gcgcatcccg cctgcgccct
     2281 ggacaatcgg ctcaatgatc accgccgcga tttcatgacg atgcgccgcc atcaggcggg
     2341 caaagcccac catatcgcgc tcatcccatt cgccatccat gcggctttgc ggggcgggag
     2401 caaacaggtt ttctggcagg tagcctttcc acagactgtg cattgagtta tccggatcgc
     2461 acaccgacat cgcgccaaag gtatcgccat gataaccatt gcggaaggtc agaaaacgct
     2521 ggcgcgcttc gcctttggct tgccagtact gcaacgccat tttcatcgcc acttccaccg
     2581 ctacggaacc ggagtccgcg agaaaaacgc actccagcgg ttgcggcgtc atcgccacca
     2641 gtttgcggca cagctcaatg gctggcgcat gggtgatacc gccaaacatc acatgcgaca
     2701 tggcatcaat ttgcgacttc atcgccgcat taagctgcgg gtgattgtag ccgtggatcg
     2761 ccgcccacca ggacgacata ccgtcaacca ggcgtttgcc gtcagacaaa atcagctcgc
     2821 aaccttcggc gctcaccacc ggataaaccg gcagagggga ggtcatggat gtgtatgggt
     2881 gccagatatg gcgttggtca aaggcaagat cgtccgttgt cataatcgac ttgtaaacca
     2941 aattgaaaag atttaggttt acaagtctac accgaattaa caacaaaaaa cacgttttgg
     3001 agaagcccca tggctcaccg cccacgctgg acattgtcgc aagtcacaga attatttgaa
     3061 aaaccgttgc tggatctgct gtttgaagcg cagcaggtgc atcgccagca tttcgatcct
     3121 cgtcaggtgc aggtcagcac gttgctgtcg attaagaccg gagcttgtcc ggaagattgc
     3181 aaatactgcc cgcaaagctc gcgctacaaa accgggctgg aagccgagcg gttgatggaa
     3241 gttgaacagg tgctggagtc ggcgcgcaaa gcgaaagcgg caggatcgac gcgcttctgt
     3301 atgggcgcgg cgtggaagaa tccccacgaa cgcgatatgc cgtacctgga acaaatggtg
     3361 cagggggtaa aagcgatggg gctggaggcg tgtatgacgc tgggcacgtt gagtgaatct
     3421 caggcgcagc gcctcgcgaa cgccgggctg gattactaca accacaacct ggacacctcg
     3481 ccggagtttt acggcaatat catcaccaca cgcacttatc aggaacgcct cgatacgctg
     3541 gaaaaagtgc gcgatgccgg gatcaaggtc tgttctggcg gcattgtggg cttaggcgaa
     3601 acggtaaaag atcgcgccgg attattgctg caactggcaa acctgccgac gccgccggaa
     3661 agcgtgccaa tcaacatgct ggtgaaggtg aaaggcacgc cgcttgccga taacgatgat
     3721 gtcgatgcct ttgattttat tcgcaccatt gcggtcgcgc ggatcatgat gccaacctct
     3781 tacgtgcgcc tttctgccgg acgcgagcag atgaacgaac agactcaggc gatgtgcttt
     3841 atggcaggcg caaactcgat tttctacggt tgcaaactgc tgaccacgcc gaatccggaa
     3901 gaagataaag acctgcaact gttccgcaaa ctggggctaa atccgcagca aactgccgtg
     3961 ctggcagggg ataacgaaca acagcaacgt cttgaacagg cgctgatgac cccggacacc
     4021 gacgaatatt acaacgcggc agcattatga gctggcagga gaaaatcaac gcggcgctcg
     4081 atgcgcggcg tgctgccgat gccctgcgtc gccgttatcc ggtggcgcaa ggagccggac
     4141 gctggctggt ggcggatgat cgccagtatc tgaacttttc cagtaacgat tatttaggtt
     4201 taagccatca tccgcaaatt atccgtgcct ggcagcaggg ggcggagcaa tttggcatcg
     4261 gtagcggcgg ctccggtcac gtcagcggtt atagcgtggt gcatcaggca ctggaagaag
     4321 agctggccga gtggcttggc tattcgcggg cactgctgtt tatctctggt ttcgccgcta
     4381 atcaggcagt tattgccgcg atgatggcga aagaggaccg tattgctgcc gaccggctta
     4441 gccatgcctc attgctggaa gctgccagtt taagcccgtc gcagcttcgc cgttttgctc
     4501 ataacgatgt cactcatttg gcgcgattgc ttgcttcccc ctgtccgggg cagcaaatgg
     4561 tggtgacaga aggcgtgttc agcatggacg gcgatagtgc gccactggcg gaaatccagc
     4621 aggtaacgca acagcacaat ggctggttga tggtcgatga tgcccacggc acgggcgtta
     4681 tcggggagca ggggcgcggc agctgctggc tgcaaaaggt aaaaccagaa ttgctggtag
     4741 tgacttttgg caaaggattt ggcgtcagcg gggcagcggt gctttgctcc agtacggtgg
     4801 cggattatct gctgcaattc gcccgccacc ttatctacag caccagtatg ccgcccgctc
     4861 aggcgcaggc attacgtgcg tcgctggcgg tcattcgcag tgatgagggt gatgcacggc
     4921 gcgaaaaact ggcggcactc attacgcgtt ttcgtgccgg agtacaggat ttgccgttta
     4981 cgcttgctga ttcatgcagc gccatccagc cattgattgt cggtgataac agccgtgcgt
     5041 tacaactggc agaaaaactg cgccagcaag gctgctgggt cacggcgatt cgcccgccaa
     5101 ccgtacccgc tggtactgcg cgactgcgct taacgctaac cgctgcgcat gaaatgcagg
     5161 atatcgaccg tctgctggag gtgctgcatg gcaacggtta ataaacaagc cattgcagcg
     5221 gcatttggtc gggctgccgc acactatgag caacatgcag atctacagcg ccagagtgct
     5281 gacgccttac tggcaatgct tccacagcgt aaatacaccc acgtactgga cgcgggttgt
     5341 ggacctggct ggatgagccg ccactggcgg gaacgtcacg cgcaggtgac ggccttagat
     5401 ctctcgccgc caatgcttgt tcaggcacgc cagaaggatg ccgcagacca ttatctggcg
     5461 ggagatatcg aatccctgcc gttagcgact gcgacgttcg atcttgcatg gagcaatctc
     5521 gcagtgcagt ggtgcggtaa tttatccacg gcactccgcg agctgtatcg ggtggtgcgc
     5581 cccaaaggcg tggtcgcgtt taccacgctg gtgcagggat cgttacccga actgcatcag
     5641 gcgtggcagg cggtggacga gcgtccgcat gctaatcgct ttttaccgcc agatgaaatc
     5701 gaacagtcgc tgaacggcgt gcattatcaa catcatattc agcccatcac gctgtggttt
     5761 gatgatgcgc tcagtgccat gcgttcgctg aaaggcatcg gtgctacgca tcttcatgaa
     5821 gggcgcgacc cgcgaatatt aacgcgttcg caattgcagc gattgcaact ggcctggccg
     5881 caacaacagg ggcgatatcc tctgacgtat catctttttt tgggagtgat tgctcgtgag
     5941 taaacgttat tttgtcaccg gaacggatac cgaagtgggg aaaaccgtcg ccagttgtgc
     6001 acttttacaa gccgcgaagg cagtaggtta tcggacggca ggttataaac cggtcgcctc
     6061 tggcagcgaa aagaccccgg aaggcttacg taatagtgac gcgctggcgt tacagcacaa
     6121 cagcagcctg cagctggatt acgcaacggt aaatccttac accttcgcag aacccacctc
     6181 gccgcacatc atcagcgcgc aagagggcag gccgatagaa tcattggtaa tgagcgccgg
     6241 attacgcgcg cttgaacaac aggctgactg ggtgttagtg gaaggtgctg gcggctggtt
     6301 tacgccgctt tctgacactt tcacttttgc agattgggta acacaggaac aactgccggt
     6361 gatactggta gttggtgtga aactcggctg tattaatcac gcgatgttga ctgcgcaggc
     6421 aatacaacac gccggactga ctctggcggg ttgggtggcg aacgatgtta cgcctccggg
     6481 aaaacgtcac gctgaatata tgaccacgct cacccgcatg attcccgcgc cgctgctggg
     6541 agagatcccc tggcttgcag aaaatccaga aaatgcggca accggaaagt acataaacct
     6601 tgccttgttg tagctatcct gtatttggtt aaattgcgag cgagatcgcg tcttcgattg
     6661 actgcaattt aaccaattaa attctaaaat aatcacgaaa aaaattttac ttccgcctca
     6721 tgcggcgaat gtgggaattg cccaggcggc gggggatagg ggctggagac agttatccac
     6781 tattcctgtg gataaccatg tgtattagag ttagaaaaca cgaggcaagc gagagaatac
     6841 gcggcttgc
//
LOCUS       DBANUU010000198         6818 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000198 DBANUU010000000
VERSION     DBANUU010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.201"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(136..804)
                     /gene="artM"
                     /locus_tag="ACIE68_004321"
     CDS             complement(136..804)
                     /gene="artM"
                     /locus_tag="ACIE68_004321"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFX5910531.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(804..1520)
                     /gene="artQ"
                     /locus_tag="ACIE68_004322"
     CDS             complement(804..1520)
                     /gene="artQ"
                     /locus_tag="ACIE68_004322"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFX5910532.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1527..2258)
                     /gene="artI"
                     /locus_tag="ACIE68_004323"
     CDS             complement(1527..2258)
                     /gene="artI"
                     /locus_tag="ACIE68_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFX5910533.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2276..3004)
                     /gene="artP"
                     /locus_tag="ACIE68_004324"
     CDS             complement(2276..3004)
                     /gene="artP"
                     /locus_tag="ACIE68_004324"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFX5910534.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3222..3737)
                     /gene="ybjP"
                     /locus_tag="ACIE68_004325"
     CDS             complement(3222..3737)
                     /gene="ybjP"
                     /locus_tag="ACIE68_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFX5910535.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKKGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3863..4186
                     /gene="ybjQ"
                     /locus_tag="ACIE68_004326"
     CDS             3863..4186
                     /gene="ybjQ"
                     /locus_tag="ACIE68_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFX5910536.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4183..5013
                     /gene="amiD"
                     /locus_tag="ACIE68_004327"
     CDS             4183..5013
                     /gene="amiD"
                     /locus_tag="ACIE68_004327"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFX5910537.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(5010..6023)
                     /gene="ybjS"
                     /locus_tag="ACIE68_004328"
     CDS             complement(5010..6023)
                     /gene="ybjS"
                     /locus_tag="ACIE68_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFX5910538.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6122..>6818)
                     /locus_tag="ACIE68_004329"
     CDS             complement(6122..>6818)
                     /locus_tag="ACIE68_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFX5910539.1"
                     /translation="FLNVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAFDD
                     AVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKTSASLAALWQVVNQ
                     IGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEKQL
                     TLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRGM
                     AKQIARLAEQSTD"
BASE COUNT         1646 a         1793 c         1801 g         1578 t
ORIGIN      
        1 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
       61 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga
      121 atgtacgcag ctacattaat ttcgccgttc gaatgccagt gctttgcgct cgatcagacg
      181 catcatcagc gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac
      241 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
      301 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
      361 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
      421 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
      481 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
      541 tgacaacaaa tgccacagtg ccggatactc ctgcaaagtc ggaaactggc ccggcccgta
      601 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
      661 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
      721 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgta gccctttcat
      781 cagttcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
      841 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
      901 acaatgtacc aggtaaatgg ttcctgggta cgagtagcga tgctttttgt ttgcagcatt
      961 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
     1021 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
     1081 gccgatttcg acagccccag cgcctgacca gactcccact gacccaccgg caccgctttc
     1141 aacgcgcccc gaagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
     1201 ccacaaagga acgggctaac gtcgaagttc tcaatgtcca tctgcactgg gatctgcacg
     1261 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gcagctgcga ggagccaaaa
     1321 tagataaaca gcaccaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
     1381 cctgcccacg cgacaggacg ccattttgcc gactcccata ccgcaaagaa catcgccagc
     1441 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
     1501 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
     1561 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagctcagtg
     1621 ttgccctgac gtaccgcgat gccgaggcca gtgccgaagt aatctttatc ggtcactttg
     1681 tcgcccaccg ccgccagttt cgggttatct ttcagccact cagtgaccac tgcggtgtca
     1741 ccgaagacgc cgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
     1801 tacggaacgg tagtgatttc cgggtgctta tccataatga atttctggtg tgtcgtcccg
     1861 ttctgtacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
     1921 cccacaaaca gggcagagtt gtcatagtac ggggtggtaa acagtacctg cttttcacgc
     1981 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
     2041 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagc
     2101 gcctgtgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
     2161 ggcggatagg aggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
     2221 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
     2281 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
     2341 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
     2401 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
     2461 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
     2521 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
     2581 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
     2641 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
     2701 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
     2761 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
     2821 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
     2881 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
     2941 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
     3001 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttgt
     3061 cagaataatt atgcaatatt tctgctttaa aagttaaaag caaagcgcat tattcaataa
     3121 acatagcaca aaataacggg ggcggtggtc ggcgagcata aatgtcggca ttcctcacga
     3181 aatgccggac aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
     3241 tgacggagcg tacccgccgt ggcatgaacg ctgccgccta aatagcgtac atcgtcgatc
     3301 acccaacact ggccttcctg aatcattaac acttcatcct gccagctctg atcacccttt
     3361 tttagatcca cacgcagagg aatatttcgc gcatcacgat tcgggattgt tgaggcactg
     3421 gcaacacggg cgctctctgg caaggttgtg cgactggaga atggatcgtt ggtcagtagt
     3481 tcccgatggt tattatcccg ggaggcatcg ctaagcagtg tcgccagttt gtcgctcaga
     3541 taagggcgca aggcggtgat gtcgttgctg cggtgcaaaa tgcggtagtc ataaaattgc
     3601 tgggccacgt tatccgggcc tccttcaacg caaggaccac tgcgtgtgcc gttatcttta
     3661 taagctggag tgactgtggt gcaggcactg aggagcagtg cgcaggggat aagcattgtc
     3721 aatttgctgt agcgcataat gatttcctta taagcgatcg ctctgaaagc gttctacgat
     3781 aataatgata tcctttcaat aatagcgtat cagtctgata atgcttttga gatcgaaggc
     3841 ttagcaaaca aggagatcga tcatgcaatt ttctacaacc ccaactctgg aaggccagac
     3901 catcgttgaa tattgcggtg tggtgaccgg cgaagcgatt ttaggtgcca atattttccg
     3961 tgatttcttt gccggtatcc gcgatatcgt tggcggacgt tccggtgcgt atgaaaaaga
     4021 actgcgtaaa gcacgggaga tcgcctttga ggaattaggc tcccaggcgc gggcgctggg
     4081 ggccgatgcc gtcgtcggta ttgatatcga ctacgaaacg gtcgggcaaa acggcagtat
     4141 gctgatggtt agcgtcagcg gtacggcggt gaaaacgcgt cgatgagaag agtcttctgg
     4201 ctggtcgctg ccgctctgtt attggcaggg tgtgcaggcg aaaaaggcat tgtcgagaaa
     4261 gagggatatc agcttgatac ccgacgccag gcgcaggcgg cgtatccgcg cattaaagtg
     4321 ctggtgatcc actacaccgc agatgatttt gatagctcgc tggcgacact gaccgataag
     4381 caggtcagct cgcattatct ggtccctgcg gtaccaccgc gatacaacgg taaaccgcgc
     4441 atctggcaac tggtgccgga acaagaactg gcctggcatg cggggattag cgcctggcgc
     4501 ggggcaacgc gccttaacga cacctctatt ggcattgagc tggaaaaccg tggctggcaa
     4561 aaatcggccg gagtgaaata ttttgccccg tttgaaccgg cacagattca ggcgcttatt
     4621 ccactggcga aagatattat tgcccgttat cacatcaagc cggaaaacgt agtggcacat
     4681 gcggatatcg caccgcagcg caaagacgat ccggggccat tatttccctg gcagcaactg
     4741 gcgcagcagg ggattggtgc ctggccggat gcgcagcggg ttaactttta ccttgccggg
     4801 cgcgcgccgc acacaccggt agataccgcg tcattgctgg agcttttggc gcgctacggt
     4861 tatgacgtta aacctgatat gacaccgcgc gagcagcggc gcgtgattat ggcattccag
     4921 atgcatttcc gcccgacgtt atataacggc gaagcggatg cagaaactca ggcgattgcc
     4981 gaagcattgc tggagaaata cgggcaggat tagcgcggca gttttccgtg gtcgcgtagc
     5041 caggcggcag ttttctcgat accttcatcc agggtgatga ccggctgata acctaactct
     5101 tcctgcgcac gcgtaatatc cagcgtaaag tcaaaattca acttggagac gccgtagtgg
     5161 gtcagcggcg gctcttttgc tgacttgcgg cctaaacgct ccatgctgcg ggcgatcata
     5221 tccagcatcg ggtaggggac ggaacgaata cgacagtcaa tattcaactc gtcgatcagc
     5281 ttctgcacga tgctgcgcag tgtgcgatgc tcgccgttgg tgatgttgta cacacgcccg
     5341 gaaggtagct tatcgcaggc ttcctggctt gccagccaca ttgcgtgcac ggcattttca
     5401 tagtaggtca tatcgaccag cgcactgccg ccatgcggta acagaatact gccgtagtgg
     5461 tgcatcatat gcgccagacg gggaataaag actttatcgt gcggtccgaa cagactttgt
     5521 gggcgcagaa tagtaaagcg cgtttgtgga ttcgcctgcg aaagcatatt gatcacttct
     5581 tcgctggctg ctttgctgcg ggcaaactcg ttggcgaagc ggtgagggcg aaaatcttct
     5641 ttaatatcgc gatggtggtg ataatcgaag tacagggagg gggaagagat atgaataaag
     5701 ttacgcacac cccaggcgac agcccattca cccaggcggc gagtggcgcg aacgttagcc
     5761 agatcgaaag cctgttgtgt cccccagggt gaggtaaagc tggagcagtg ccacagcgta
     5821 tcaatgcccg cgagcatcac tttagcttgt gatgaaacca gctcggtcag atccgccgga
     5881 acaaactctg cgcccatttt ttccagcaat ttgcccattg cctcgttgcg accggtcgct
     5941 cgcacgctga tgcctttctg gcataaaaac tctaccgcgt ttcgacctaa gccgctggtg
     6001 gcgccggtaa ccagtacctt catatcaatc cactgttgtt gagaaaataa cgtgcgcatt
     6061 cttccgtgat ttcccccatg atgcaatggg aaacatgaaa gaataacgca ggttttgtcg
     6121 attaatctgt gctttgttct gccagtctgg cgatttgttt tgccattccg cgaaaaataa
     6181 acagatgcgc ggggatcatc aataaccagt aaaacagccc cggcataccg tgcggatgcc
     6241 agaaagcgcg gacatcgata gtacgatagt cgcctttatc ttccaggcta aaacacagtc
     6301 gtcccagccc cggcgctttc atgccaaata acaacgtaag ttgtttttcc ggttcaacga
     6361 caatcacttt ccagctatct accgcatcgc cagtctgtaa atattcgcgc tccgggcggc
     6421 ctttcgccag cttatgaccg atcgcgcggt ccatcaacgc ccgtgtctgc cacaaaatat
     6481 tgccaaagaa ataacgctct ttaccgccga tttggttcac tacctgccat aaagcagcaa
     6541 ggctggcgga cgttttaacg gtaaaccccg cctgtttggc aaaataaccg tactccggtc
     6601 gccagcgggc aaaggcctga gcgtcgtagc cccagtcgct ggagttgacc agtttttcct
     6661 cctctttcaa cgtgctacgt accgcgtcat cgaaagcgat cagccgttgt gggatgagtg
     6721 cacgtagcgc ggtatcatcc gccagcagat cgtgtttcag cccctgaatc aacgccctgg
     6781 cggtggtggg cggtacggaa gtaatcacat tgagaaac
//
LOCUS       DBANUU010000199         6791 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000199 DBANUU010000000
VERSION     DBANUU010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.202"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..110)
                     /locus_tag="ACIE68_004330"
     CDS             complement(<1..110)
                     /locus_tag="ACIE68_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFX5910540.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQIQHVLDD"
     gene            complement(107..541)
                     /locus_tag="ACIE68_004331"
     CDS             complement(107..541)
                     /locus_tag="ACIE68_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFX5910541.1"
                     /translation="MDKPTDWRSGTRRIFSNEFKLHMVELASKPNANVAQLAREHGVD
                     NNLIFKWLRLWQREGRISRRMPPTIVGPTVSQSFPASPTLVPVELIDTPRCATDAPAP
                     EALSVACAASCHVEFHYGKMMLENPSPELLTVLIRELTGRGR"
     gene            872..2842
                     /locus_tag="ACIE68_004332"
     CDS             872..2842
                     /locus_tag="ACIE68_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HFX5910542.1"
                     /translation="MYMNVIRNVICTLIILPIGLQAETSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVVTNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGIRQLSDMAKSSGTYLDPALVKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGLSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVGITDSNKAGASLRLYRNNTTEPGNPTQTHGDSGLRDRKTVQNDV
                     QFWYQYAPVDNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSYPVNIIVGSRYDR
                     YKSFNPRAGELKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTGNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKGFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            2849..3640
                     /locus_tag="ACIE68_004333"
     CDS             2849..3640
                     /locus_tag="ACIE68_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFX5910543.1"
                     /translation="MMKNTGYILALCLTASGHVLAHDVWITGKQAENNITAEIGYGHN
                     FPSKGTIPDRRNFFENPRIYNGKETITLKPASTDYVYKTESASKDNGYVLSTYMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGVQQMPAQLYQSPTGHELEIIPLS
                     DISRFSEDVKLQVLYKTSPLAGAIMELDSVSYLKSSRHTHAVEHKHPVHKAELTFVTN
                     EDGIVTVPSLHIGQWLAKVQNKKSFQDKSLCDETVDVATLSFSRN"
     gene            complement(3703..4302)
                     /locus_tag="ACIE68_004334"
                     /pseudo
     CDS             complement(3703..4302)
                     /locus_tag="ACIE68_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            4302..4502
                     /locus_tag="ACIE68_004335"
                     /pseudo
     CDS             4302..4502
                     /locus_tag="ACIE68_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            5175..6683
                     /gene="ltrA"
                     /locus_tag="ACIE68_004336"
     CDS             5175..6683
                     /gene="ltrA"
                     /locus_tag="ACIE68_004336"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFX5910544.1"
                     /translation="MQRKLATWAVTDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFSKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT         1765 a         1647 c         1801 g         1578 t
ORIGIN      
        1 ttatcatcca gcacatgctg tatctgctcc cccagaccgt tgaaggattt acgcatatca
       61 gtaacgccgg caacgagcca gatacgggta cctgatggga gtgaaatcat cgtcccctcc
      121 cggtcagctc acggatcaac accgtgagca gctctggtga aggattttcc agcatcattt
      181 taccgtaatg gaattccaca tggcaggaag ctgcacaagc aactgataat gcctccggag
      241 caggagcatc tgtagcacag cgcggggtgt cgataagttc cactggaacc agagtcggag
      301 aggccggaaa agattgtgat actgtagggc ctacaatagt tggaggcatt ctacgagaaa
      361 tacgtccttc tctttgccag aggcgtagcc atttaaaaat caggttgtta tcaacgccat
      421 gttcccgggc cagttgtgca acattggcat ttggtttcga agccagttca accatatgaa
      481 gtttaaattc attagaaaat atccggcggg ttccggagcg ccagtctgta ggtttatcca
      541 tcaatagaac tccgtctaat ttagaaggag ctctaattta gtctgcaagg agcaggtgtt
      601 acagacggca ttggctttac gcttacgact atcacttatt taagtgatat tggttgtctg
      661 gagattcagg gggccagtct aaattccgta ttgctggttc tttatcggta gaaaaatcaa
      721 tatccatagc atcggtcgca ggcgctgata cattgcagag cgaccgggta ttagagtcgc
      781 tattcactgg ttatcactga acgctgcgtg atcagggttg cattaataaa atgatatgtt
      841 ataacatatc aatattaaca tatattttcg catgtatatg aacgtaatca gaaacgtcat
      901 ttgtacatta atcatactcc cgatagggtt acaggcagag accagtcatt cttctatggc
      961 taaagataca atcaccgttg tcgcgacagg aaatcagaac acggtatttg aaacgccgtc
     1021 aatggtcagt gtcgtcacga atgacacgcc atggagtcag aatgcggtca catcagccgg
     1081 catgctgaaa ggtgttgccg gtctcagcca gactggtgca ggacggacca atgggcagac
     1141 ctttaatcta cgcggctatg acaaaagcgg ggtacttgtt cttgttgacg gcatccgtca
     1201 actcagtgac atggcaaaaa gcagtggcac ttatctggat ccggcactcg tcaaacgtat
     1261 cgaagttgtc cgcggaccaa actccagtct gtacggcagt ggcgggctgg gaggtgttgt
     1321 ggacttcaga actgccgatg cagcagattt ccttcccccc ggagagacaa acggtttaag
     1381 tctgtgggga aatatcgcca gtggtgacca cagcacaggc tcggggctca cctggttcgg
     1441 taaaaccgga aaaacagatg cgctcctttc tgtcattatg cgtaaaagag gtaatatcta
     1501 tcaaagtgat ggtgagcgcg cacctaacaa ggaaaaacct gcagccctgt ttgcgaaagg
     1561 ctctgtcggt ataacagaca gtaacaaagc aggtgccagc ttgcgtctct accggaataa
     1621 caccactgaa ccgggcaatc ccactcagac acatggtgac agcggcctgc gtgacagaaa
     1681 aacagtacaa aatgacgtac agttctggta ccagtacgct cctgtggata acagcctcat
     1741 caatgtaaag tcaactttat atctcagtga tatcactatc aagacaaacg gtcacaacaa
     1801 aacggcagaa tggagaaaca acagaacctc cggtgttaat gttgtcaaca ggagtcatac
     1861 tctgattttt ccgggagccc atcagttaag ttatggcgct gaatattacc gtcagcagca
     1921 gaagccagaa ggctctgcca cactatatcc ggaaggaaac attgacttta catcgttgta
     1981 tttccaggat gaaatgacaa tgaaaagcta cccggttaac attatcgtcg gttcccgcta
     2041 tgaccggtac aagagcttca atccccgtgc cggagaactg aaagccgaac gcctgtcccc
     2101 aagggcggcg atttcagtct caccgacaga ctggctgatg atgtacggct ccatatcctc
     2161 tgcattccgt gcccccacaa tggcagaaat gtacagggat gatgtacatt tttaccgcaa
     2221 gggtaaaccc aattactggg tacctaacct taatctgaaa ccagaaaata acattacccg
     2281 tgagattggc gcaggtattc aactggacgg cctgcttaca ggcaatgatc ggctgcagtt
     2341 gaaaggcgga tatttcggaa cggatgccag aaactatatt gccacgcgcg tggatatgaa
     2401 acggatgcgt tcttattctt ataatgtatc ccgggcccgt atctggggat gggatatgca
     2461 gggaaattac cagtctgatt attttgactg gatgctttct tataaccgca cggaaagtat
     2521 ggatgccagc agcagggaat ggctgggctc cggcaatcct gacacactca tcagtgacat
     2581 cagtatacct gttggtcatg gaggagtttc tgccggatgg cgtgctgaac tttcggcagc
     2641 agccacgcat gtgaaaaaag gcgatcccca tcaggctggc tatgccgtac attccttttc
     2701 actgtcttat aagcctgtaa gtgttaaagg ctttgaggca tcagtgactc tggataatgc
     2761 cttcaacaag cttgccatga atggcaaagg agtgccgctt tcaggcagaa ctgtcagtct
     2821 ttatacccgt tatcagtggt aacaaatcat gatgaaaaat acaggttata tcttagctct
     2881 ttgtctgaca gcatcggggc atgtcctggc ccacgatgtc tggattacag gtaaacaggc
     2941 agagaacaac attaccgcag agattggtta tggtcataat ttcccctcaa agggcacaat
     3001 tcctgacaga aggaatttct ttgaaaatcc ccggatttat aacgggaaag agacaataac
     3061 actgaagcca gcgtccacgg attatgtcta taaaactgag tctgcaagca aagataatgg
     3121 ctacgttttg tcaacatata tgaaaccggg atactggtcg agaacctctt caggatggaa
     3181 accggtcagc cgggagggca gaaatgatgt ggcttactgt gagtttgtca ctaaatatgc
     3241 gaaatctttt attcctggtg tacagcagat gccagcacaa ctctatcagt ctccaacggg
     3301 gcatgagctt gaaatcattc cgttatccga tataagtcgt ttcagtgaag atgtgaagct
     3361 gcaagtcctg tataaaacgt ccccgctcgc cggagctatc atggagcttg actcggtcag
     3421 ttatctgaaa tcatcccgtc atactcatgc agttgagcac aaacatcctg ttcataaagc
     3481 agaactcacc tttgtaacta atgaggatgg tatcgtcaca gtaccttctc ttcatattgg
     3541 acagtggctg gcgaaagtcc aaaataagaa aagttttcag gacaaaagcc tgtgtgatga
     3601 aactgtcgat gtggcaactt taagtttctc ccgaaattaa caatactatt ccctgccctg
     3661 aaattgtaag cgtgcagcaa gaaccgtatt gacggggatg tgttattcag tcggcagtgc
     3721 tacgcgccag gggagcagtt cgccgacccg gtttatcggc cagtcggcta tgacgtcaag
     3781 gacatagcgg aggtagcttt ctggctccac tccattcagt ttgcacgtcc cgatcaggct
     3841 gtacagcagc gctccccgct ctcctccatg atccgaaccg aagaacaggt agtttttgcg
     3901 gcccagactg accatccgca acgcattttc agcgatgtta ttgtccgcct cagcccagcc
     3961 atcatctgca tagtacgtca gcgccggcca ctggttcagg gcgtatgcga acgctttcgc
     4021 cagttctgag tgtcgcgaca gggttttcat cttttcacgc agccagcttt ccagggattt
     4081 cagcagcggt ttcgttttca actgacgttc ggcaaggcgc tgctccgccg tcattcccct
     4141 tatctctgcc tcgatggcgt acagttcgcc gatccgtttc agcgcttcct ccgtcagggc
     4201 tgacggggtg cgaacgtgca catcgtggat tttacggcgg gcgtgagccc aacaggcggc
     4261 ttccgttatc cggccatccc ggtacagctc gttgaacccg gcgtcagttg ttacgggaag
     4321 gaatcagggt agccagatgt acagtggcgc gcctcatggc ggttatggga cttgccgatg
     4381 ttctccgggg taaaaaggtc cgcactaccg tcagccggaa agccgttgcc gcaggcgacc
     4441 gcgtaaaccg tcagtttgtg gcagaacgtc ctgaccagtt gtgggtggct gattttactt
     4501 acggtgtgcc cggagttcag ggcgggcatg gatgcttaaa tgaaccgcga gtctgtctgg
     4561 aatattgaac cggtaactca cgatgagaaa cccaacaatc ccaccgggtg tgacggtgga
     4621 gaacctgagc ggcagtgacc tgcggcatgc ccgcagggtg atgtaacccg ctgacaacgg
     4681 ggattgaggc gagatcacta agccgagatg atcctcaagg ttaagtactg aaaggctgaa
     4741 gaacatgaac ccgttaatcc gcctctgtgg gttgaaaacg tcaccacggc ctacgtgatc
     4801 tgacaggccg tgcaggagga actggcagtg atacgtaagc actgccggtc gaaggtgttt
     4861 tgacatgtat gcgaaacact ggggcagcag cgtctatcac gcttgcgttg ctgacttctg
     4921 ccaacttgcg gcaagcaagg ataaagagtg cgacgggcag cctcctcagt atgcctgagt
     4981 ccaggcaggt aaaccgggga aggtcagcga cggatgttaa gggggcatgg ctccgatgac
     5041 gcgctggctg gcggagcttc cgtagtagtc cgcgatgggg aaagcccatt acatggcgaa
     5101 gggaagcagt ttgaatgtgt ttgcgacgtg aattaactga cctaacgagg tgaagacctt
     5161 tgataatcag cgaaatgcaa cgcaagcttg ccacatgggc agtcaccgat ccgtccctac
     5221 ggattcaacg gctgctgcgt ctgataacac aaccagaatg gctggctgaa gcggcgcgga
     5281 tcacgctttc atcaaagggg gcccataccc ccggcgttga tggcgtgaac aaaacaatgc
     5341 tacaggccag actggctgtt gagctgcaaa tcctcaggga tgaattactc tcaggccact
     5401 accagccctt gcccgccaga cgggtttaca tccctaaaag caacggcaaa ctgcgaccac
     5461 tgggtatccc cgcgttgcgg gatcgtattg ttcagcgggc catgctgatg gcgatggagc
     5521 cgatatggga gagtgatttt catacgctct catatggctt ccggcctgag cgcagtgtcc
     5581 accacgctat ccgcacggtg aaattacagc tcacagactg tggtgaaacc cggggacgct
     5641 gggtgattga aggcgacctg tccagttact tcgacaccgt acatcatcga ctgctgatga
     5701 aggctgtacg ccgcaggatc agtgacgcac gtttcatgac tctgctgtgg aaaaccatca
     5761 aggcgggaca tatcgatgtc ggtctctttc gggcggccag tgaaggtgtg ccacagggcg
     5821 gtgttatatc gccgctatta tcgaacatca tgctgaatga gttcgatcaa tacctgcatg
     5881 agcgctacct gagcgggaaa gccagaaaag atcggtggta ctggaataac agtatccaac
     5941 ggggccgaag tacggcggtc agagaaaact ggcagtggaa acccgcggtg gcgtactgcc
     6001 gctatgccga tgattttgtc ctcatcgtca aaggcaccaa agcacaggcg gaagccatca
     6061 gggaggagtg tcggggtgtg ctcgaaggca gtctgaaact caggctgaac atggataaga
     6121 ctaaaatcac ccatgttaat gacggcttta tctttctggg gcacaggatc attcgcaaac
     6181 gcagtcgtta tggcgagatg cgagtggtct caacgatccc gcaggagaaa gccagaaact
     6241 tcgccgcatc gctgacagca ctgttatcag gcaactacag tgaaagcaaa gtcgatatgg
     6301 ctgaacaact caaccgaaaa ctgaaaggct gggccatgtt ctatcagttc gttgatttta
     6361 aggccaaagt cttcagttat atcgaccgtg tcgtgttctg gaagctggct cactggctgg
     6421 cccgcaaata ccgtacaggt atcgcttccc tgatgaggtg gtggtgtaaa tcaccgaaac
     6481 cgggtcagag caaaacgtgg gttttattta gtaaaaccaa tcacggcaag ctcagcggcg
     6541 aaatactgta ccggttggtg gggcaaggca agaagctgtt ccgctggcgg ctacccgaag
     6601 gtaatcccta tctgaggacg gagaccagaa acacgtatac atcgcgcttt acagaagtgg
     6661 caatggcgtt cgccagcatt taaatggaga gccggatgcg ctgaaaggtg cacgtccggt
     6721 tcggggagga gaggcaggga aatagtccga ctacgccctg cctcttactc tactcagcac
     6781 atggctgggg g
//
LOCUS       DBANUU010000200         6766 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000200 DBANUU010000000
VERSION     DBANUU010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.203"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..384)
                     /locus_tag="ACIE68_004337"
     CDS             complement(<1..384)
                     /locus_tag="ACIE68_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5910545.1"
                     /translation="MKKIIGLCLPVMLGAVLMSQHAHAADNLTFKGKLIIPACTVTKA
                     EVDWGNIEIQTLSQNGNHEKEFTVNMQCPYNLGTMKVTITATNTQNNNSILVPNTSTT
                     TGNGLLIYLYNRNQNGSIGSPIVFNS"
     gene            complement(411..947)
                     /locus_tag="ACIE68_004338"
     CDS             complement(411..947)
                     /locus_tag="ACIE68_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5910546.1"
                     /translation="MIKSTGALLLFAALSAGQAMASDVAFRGNLLDRPCHVSGDSLKK
                     HVVFKTRASRDFWYPPGRSPTESFVIRLENCHATAVGKIVTLTFKGTEEAALPGHLKV
                     TGVNSGRLGIALLDTDGSSLLKPGTFHNKGQGEKVTGNSLELPFGAYVVATPEALRTK
                     SVVPGDYEATATFELTYR"
     gene            complement(957..1538)
                     /locus_tag="ACIE68_004339"
     CDS             complement(957..1538)
                     /locus_tag="ACIE68_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus-assembly fibrillin subunit"
                     /protein_id="HFX5910547.1"
                     /translation="MVVNKTTAVLYLIALSLSGFIHTFLRAEERGIYDDVFTADELHH
                     YRINERGGRTGSLAVSGALLSSPCTLVSNEVPLSLRPENHSASAGAPLMLRLAGCGDG
                     GALQPGKRGVAMTVSGSLVTGPGSGSALLPDRKLSGCDHLVIHDGDTFLLCRPDRRQE
                     EMLAAWRKRATQEGEYSDARSNPAMLRLSIKYE"
     gene            complement(1575..2294)
                     /locus_tag="ACIE68_004340"
     CDS             complement(1575..2294)
                     /locus_tag="ACIE68_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFX5910548.1"
                     /translation="MIRKKILMAAIPLFVISGADAAVSLDRTRAVFDGSEKSMTLDIS
                     NDNKQLPYLAQAWIENENQEKIITGPVIATPPVQRLEPGAKSMVRLSTTPDISKLPQD
                     RESLFYFNLREIPPRSEKANVLQIALQTKIKLFYRPAAIKTRPNEVWQDQLILNKVSG
                     GYRIENPTPYYVTVIGLGGSEKQAEEGEFETVMLSPRSEQTVKSANYNTPYLSYINDY
                     GGRPVLSFICNGSRCSVKKEK"
     gene            complement(2380..4890)
                     /gene="papC"
                     /locus_tag="ACIE68_004341"
     CDS             complement(2380..4890)
                     /gene="papC"
                     /locus_tag="ACIE68_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial usher protein PapC"
                     /protein_id="HFX5910549.1"
                     /translation="MKDRIPFAVNNITCVILLSLFCNAASAVEFNTDVLDAADKKNID
                     FTRFSEAGYVLPGQYLLDVIVNGQSISPASLQISFVEPQSSGDKAEKKLPQACLTSDM
                     VRLMGLTAESLDKVVYWHDGQCADFHGLPGVDIRPDTGAGVLRINMPQAWLEYSDATW
                     LPPSRWDDGIPGLMLDYNLNGTVSRNYQGGDSHQFSYNGTVGGNLGPWRLRADYQGSQ
                     EQSRYNGEKTTNRNFTWSRFYLFRAIPRWRANLTLGENNINSDIFRSWSYTGASLESD
                     DRMLPPRLRGYAPQITGIAETNARVVVSQQGRVLYDSMVPAGPFSIQDLDSSVRGRLD
                     VEVIEQNGRKKTFQVDTASVPYLTRPGQVRYKLVSGRSRGYGHETEGPVFATGEASWG
                     LSNQWSLYGGAVLAGDYNALAAGAGWDLGVPGTLSADITQSVARIEGERTFQGKSWRL
                     SYSKRFDNADADITFAGYRFSERNYMTMEQYLNARYRNDYSSREKEMYTVTLNKNVAD
                     WNTSFNLQYSRQTYWDIRKTDYYTVSVNRYFNVFGLQGVAVGLSASRSKYLGRDNDSA
                     YLRISVPLGTGTASYSGSMSNDRYVNMAGYTDTFNDGLDSYSLNAGLNSGGGLTSQRQ
                     INAYYSHRSPLANLSANIASLQKGYTSFGVSASGGATITGKGAALHAGGMSGGTRLLV
                     DTDGVGGVPVDGGQVVTNRWGTGVVTDISSYYRNITSVDLKRLPDDVEATRSVVESAL
                     TEGAIGYRKFSVLKGKRLFAILRLADGSQPPFGASVTSEKGRELGMVADEGLAWLSGV
                     TPGETLSVNWDEKIQCQVNVPETAISDQQLLLPCTPQK"
     gene            complement(4949..5509)
                     /gene="papH"
                     /locus_tag="ACIE68_004342"
     CDS             complement(4949..5509)
                     /gene="papH"
                     /locus_tag="ACIE68_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial minor subunit PapH"
                     /protein_id="HFX5910550.1"
                     /translation="MYFWSTLLHAVSSEPFPPPGMSLPEYWGEEHVWWDGRAAFHGEV
                     VRPACTLAMEDAWQIIDMGETPVRDLQNGFSGPERKFSLRLRNCEFNSQGGNLFSDSR
                     IRVTFDGVRGETPDKFNLSGQAKGINLQIADARGNIARAGKVMPAIPLTGNEEALDYT
                     LRIVRNGKKLEAGNYFAVLGFRVDYE"
     gene            complement(5599..6150)
                     /gene="papA"
                     /locus_tag="ACIE68_004343"
     CDS             complement(5599..6150)
                     /gene="papA"
                     /locus_tag="ACIE68_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbria major subunit PapA"
                     /protein_id="HFX5910551.1"
                     /translation="MIKSVIAGAVAMAVVSFGVNAAPTTPQGQGRVTFNGTVVDAPCS
                     ISQKSADQSIDFGQLSKSFLANDGQSKPMNLDIELVNCDITAFKNGNAKTGSVKLAFT
                     GPTVSGHPSELATNGGTGTAIMIQAAGKNVPFDGTEGDANLLKDGDNVLHYTAVVKKS
                     SDGNAQITEGAFSAVATFNLSYQ"
     gene            complement(6356..6670)
                     /locus_tag="ACIE68_004344"
     CDS             complement(6356..6670)
                     /locus_tag="ACIE68_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
                     /protein_id="HFX5910552.1"
                     /translation="MAHHEVISRSGNEFLLNIRENVLLPGSMSEMHFFLLIGISSIHS
                     DRVILAMKDYLVGGHSRKEVCEKYQMNNGYFSTTLGRLIRLNALAARLAPYYTDESSA
                     FD"
BASE COUNT         1736 a         1849 c         1424 g         1757 t
ORIGIN      
        1 cgaattaaaa actataggtg aaccaataga accattttga tttctgttgt aaagatagat
       61 aagcaatcca ttacctgtcg tagttgatgt attaggcacc agtatagaat tgttattctg
      121 agtattcgtg gctgttatcg taaccttcat tgttcccaga ttataaggac actgcatatt
      181 cacagtaaac tctttttcgt gatttccatt ttgactcagg gtctgaatct ctatatttcc
      241 ccagtcaacc tctgcctttg ttacagtaca ggcaggaata atcagttttc ctttaaaggt
      301 cagattatca gctgcatgtg catgctgaga cattaacact gcccccagca ttaccggaag
      361 acacaaacct attatctttt tcatctgaaa tctcctgtac aaaatttttt ctaacgatat
      421 gttaattcaa acgtggctgt tgcttcataa tcaccgggta ccacactctt cgtccgcagg
      481 gcttccggcg ttgccacaac atacgcgccg aaaggaagct caagactgtt tccggtaacc
      541 ttttccccct ggcctttgtt atggaaggtg ccgggtttca gcagactgct gccatcggtg
      601 tccagcagtg caatgcctaa ccggccggaa ttcactccgg ttaccttcag atggcccggg
      661 agggccgcct cttccgtccc cttaaaggtc agggtcacaa ttttgccaac tgctgttgca
      721 tggcagtttt ccagcctgat gacaaacgac tctgtcggcg aacgtccggg cggataccag
      781 aaatccctgg acgcgcgggt tttgaagacg acatgtttct tcagactgtc accggacaca
      841 tggcagggtc tgtcaagcag attacccctg aatgccacat ctgaggccat tgcctgcccg
      901 gcagacagtg cggcaaacag taaaagagcg cctgtgcttt ttatcatcac atccccttac
      961 tcatatttta tgctcagacg cagcatggcc ggattgctcc tggcatcaga atactcaccc
     1021 tcctgtgtcg cccttttcct ccaggcggcc agcatctcct cctgccgccg gtcaggccgg
     1081 cacagtaaaa aggtatcacc atcgtgtata acaagatggt cacagccgga tagcttacgg
     1141 tcaggaagta aagcacttcc gcttccggga ccggttacca gtgagccgga gactgtcatc
     1201 gcaactcccc gttttccggg ctgaagtgca ccaccgtccc cacatcctgc cagcctcagc
     1261 atcagaggtg ctccggctga cgcagagtga ttctccggcc ggaggcttaa cggcacctca
     1321 ttactcacca gcgtgcaggg tgaggacagc agtgcaccac tgactgccag gcttccggtg
     1381 cgtccccccc gttcatttat ccggtaatga tgcaactcat ctgcagtaaa gacgtcatcg
     1441 tatatacccc gctcttcagc ccgcaggaaa gtatggatga aaccactcag cgacagtgca
     1501 ataagataca gtactgctgt tgttttattc acaaccataa tatcccaccc gcatttaacc
     1561 gttattgcgg tacattattt ctcttttttc acagagcaac ggctgccatt acagataaac
     1621 gacagtaccg ggcgaccacc atagtcgtta atataagaca gataaggggt attataattt
     1681 gccgatttta ctgtctgctc tgaacgggga gacagcatca cggtttcaaa ctcaccttcc
     1741 tctgcctgtt tttcacttcc tcccagacca ataacagtga cataataggg cgttgggttt
     1801 tcaatacgat acccaccgct gactttgttc agaattaact ggtcctgcca tacttcattc
     1861 ggtctggttt taattgctgc cgggcgataa aaaagcttta ttttggtctg taaggctatc
     1921 tgcagtacat tggccttttc actcctcggc ggtatttccc ggagattaaa ataaaacagt
     1981 gattccctgt cctgaggaag tttactgata tccggtgtgg tactcagcct gaccatgctt
     2041 ttcgcacccg gctcaaggcg ctgaaccgga ggggtggcaa taaccggccc tgtaataatt
     2101 ttttcctgat tttcattttc tatccatgcc tgagcaagat agggcagttg tttgttatca
     2161 ttggagatat caagcgtcat tgacttctca ctcccgtcaa acaccgcgcg ggttctgtcc
     2221 agcgaaacag cagcgtcagc cccggatata acaaacaggg ggatggcagc catcagaatc
     2281 ttttttcgaa tcatacttaa tttccacatt ctgtaatttc acctggtccg gaaaatggca
     2341 taaccgcatt catcagccgt taatattccg gactttcatt tatttctgag gcgtacaggg
     2401 aagcaataac tgctggtcag atattgctgt ctccggtaca tttacctgac actgtatttt
     2461 ttcatcccag tttaccgaca gggtttcccc cggcgtcacg ccactcagcc aggcaaggcc
     2521 ttcatcggcc accatgccca gttcccggcc tttttcactg gttacactgg caccaaacgg
     2581 gggctgagag ccatcagcaa gacgcagtat tgcaaacaga cgtttccctt taagcacgct
     2641 gaatttccgg taaccaatgg caccttctgt cagcgccgat tccacaacag aacgggttgc
     2701 ttccacatca tccggtaagc gcttcaggtc aacagaagtt atattccggt aataactgct
     2761 gatgtcagtc accacgcccg ttccccagcg atttgtcacc acctgcccgc catcaaccgg
     2821 tacacctccc acaccatccg tgtcaacaag aagacgtgtt ccaccggaca ttccccctgc
     2881 atgtaacgcc gcaccttttc cggtaattgt tgccccaccg gaagcactga cgccgaaaga
     2941 cgtatatcct ttctgcaggg atgcaatatt cgcggacaaa tttgccagcg gactacgatg
     3001 actgtaatag gcattaatct gacgttgcga tgtcagtcca ccgccactgt taaggccggc
     3061 gttcaggctg tagctgtcca gaccgtcatt gaacgtgtca gtgtagccgg ccatattcac
     3121 ataacggtca ttactcatac tgccactgta gctcgctgtc cccgtcccca gcggcacgga
     3181 tatacgcagg taagcagaat cgttatcacg ccccagatat ttagaccttg aggctgacaa
     3241 tccaaccgcc acaccctgca gtccgaaaac attaaagtag cggttgacgc tcaccgtata
     3301 atagtccgtt ttccgtatgt cccagtatgt ctgacggctg tactgcaggt taaaagaggt
     3361 gttccagtcc gccacgtttt tattcagcgt aacggtatac atctcttttt cccgactgct
     3421 gtaatcatta cggtagcggg cgttcaggta ctgctccatg gtcatatagt ttcgctctga
     3481 gaaacgatac ccggcgaacg taatgtcggc atccgcatta tcaaaccgtt tggagtagct
     3541 cagacgccag gattttccct gaaacgttct ctctccctca atacgggcta ctgactgcgt
     3601 gatatcagcg gaaagggtcc ccggcacacc caggtcccag ccggcaccgg ctgccagtgc
     3661 attataatca ccggcaagca cagccccgcc atacagcgac cactggttac tgagccccca
     3721 ggatgcctct ccggtcgcaa atacaggccc ttcggtctca tgcccgtatc cacgggaacg
     3781 accggagaca agtttgtacc ggacctgtcc cggacgcgtc agataaggaa ccgaggctgt
     3841 atcgacctga aaggttttct tccgtccgtt ctgttcaata acctcaacat caagacgtcc
     3901 gcgaactgaa ctgtccaggt cctgaatact gaatggccct gcggggacca tagagtcgta
     3961 cagcacccgt ccctgctgcg acaccacaac acgggcatta gtctccgcaa tcccggtaat
     4021 ctgcggtgca tagcctcgca gtctgggcgg cagcatccgg tcatcgcttt ccaggctggc
     4081 tcccgtataa ctccatgacc ggaatatatc tgagttgata ttattctcgc ccagcgtcag
     4141 gtttgcccgc caccggggaa tggcacggaa cagataaaag cgactccatg tgaaatttct
     4201 gtttgtcgtt ttttccccgt tgtagcggct ctgctcctgg cttccctgat agtcagcccg
     4261 caggcgccag ggccccagat tcccccccac agtcccgtta taactgaact gatgagagtc
     4321 tcctccctga taattacggg aaaccgtccc gttgaggtta taatccagca tcagtccggg
     4381 aatgccgtcg tcccagcgtg agggaggcag ccaggtggca tcagaatact caagccaggc
     4441 ctgcggcata ttgatgcgta atacgcccgc tccggtatca ggacgaatat ccactcccgg
     4501 caacccatga aaatccgcac actgaccatc atgccagtaa acaactttat ccagagattc
     4561 tgctgttaac cccatcagtc tgaccatatc tgatgtcaga caggcctgtg gcaatttttt
     4621 ttctgcctta tctcctgacg actgaggttc aacaaatgaa atctgtaacg atgcgggaga
     4681 aatactttgc ccgttaacaa tcacatccag aagatattgc cccggcagaa cataaccggc
     4741 ttctgaaaaa cgggtgaagt caatattttt cttgtccgct gcgtcaagta catctgtatt
     4801 aaactcaacg gcactggctg cgttacaaaa cagagacaac aatatcacac aggtaatatt
     4861 gttgactgca aaaggtattc tgtctttcat tccacgcatc accagattca caaaaaagat
     4921 aaataaccgg acatctcacc ggagtgactc actcataatc gacccggaat cccagcacag
     4981 caaaataatt tccggcttca agtttttttc cgtttctcac aattctgagg gtgtaatcca
     5041 gcgcttcttc attacccgtc aatggtattg caggcattac tttccctgcc cgggcaatat
     5101 tccccctggc atcagctatc tgcagattaa ttccttttgc ctgaccggat aaattaaact
     5161 tatccggcgt ttcaccccgg acgccatcga aagtcaccct tatccgggaa tcagagaaaa
     5221 ggttcccacc ctgactgtta aattcacagt ttctgagccg gaggctgaat tttctttcag
     5281 gtccggaaaa accattctgt aaatcccgaa ccggggtttc ccccatatca ataatctgcc
     5341 aggcgtcttc catcgccaga gtacaggcag gtctgacaac ctcaccatga aaagcagccc
     5401 tgccgtccca ccatacgtgt tcttctcccc agtattcagg aagggacatg ccgggcggag
     5461 gaaatggctc tgaagataca gcatgaagta atgtactcca gaaatacatc acaaacagaa
     5521 cacaaaaatt cttcatacct taaatgtcgt tgtcagacac agggtgggtc ttgcctcacc
     5581 ctgtaaaaat cagatgaatt actggtaact taaattaaag gttgcgactg cagaaaaagc
     5641 accttcagta atttgagcat taccatcaga tgatttctta acaacagctg tataatgcag
     5701 tacattatca ccatctttca ggagattagc gtcaccctcc gtaccatcga agggaacgtt
     5761 tttacccgca gcctgaatca taatagctgt gcccgtaccg ccgttagtag ctaactcact
     5821 aggatgacca ctaactgttg ggcctgtaaa agctaactta acgctcccag ttttagcatt
     5881 accattttta aaggcagtaa tgtcacaatt aactaattca atatccaagt tcattggctt
     5941 ggattgacca tcatttgcaa gaaaactttt tgaaagttgt ccaaaatcaa tagactgatc
     6001 agctgatttc tgagaaatgc tgcatggagc atcaacaaca gttccgttaa aggttactct
     6061 accctgcccc tgtggagtag ttggagcagc atttacacca aaagacacca ctgccatagc
     6121 taccgcaccg gcaataaccg acttaatcat aaataacaac ctctttttca ttactgaaca
     6181 caccagagat atctggcaat ttaaaatgac ccgattacct ttaataatca gatcaataca
     6241 aataatattc tcgtattttc agggcaggtt atttatcgac aaaatactga tataacctgt
     6301 ctgctttctg ataaggactt cttttgatct gtaagaaact ccggaatgcc atcattcagt
     6361 caaatgccga cgactcatct gtataataag gtgcaagcct tgctgcaaga gcattcagcc
     6421 gtataagtct cccaagtgtt gtactgaaat acccattatt catctggtat ttctcgcaga
     6481 cctccttacg ggagtgccca cctaccagat agtccttcat agccagaatg accctgtcac
     6541 tgtgaataga cgaaatacct atcagtaaaa aaaaatgcat ttcagacata gagccgggca
     6601 acagtacgtt ctcgcgtata ttcagcaaaa actcatttcc tgaccgactg atgacttcat
     6661 gatgcgccat gtttccccct tctgtcgggc ccctggatat atgcttccgg cccggaaata
     6721 ctcttcactc aataagttaa atttagtttt ttatgttgta aatatt
//
LOCUS       DBANUU010000201         6733 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000201 DBANUU010000000
VERSION     DBANUU010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.204"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..4156)
                     /gene="rhsD"
                     /locus_tag="ACIE68_004345"
     CDS             complement(<1..4156)
                     /gene="rhsD"
                     /locus_tag="ACIE68_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFX5910553.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDI
                     RLQLRDDGLILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRELTGLADRFGR
                     TLTYRREAAGDLTGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     AFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPAAPLVRYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQDRIT
                     VTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGL
                     NVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRREYDEPGRLVSETSRSGE
                     TVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTA
                     VHREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQYDAW
                     GKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSDFSYDALDRLVQQGGFDGRTQRYH
                     YDLTGKLTQSEDEGLVTLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGH
                     RVAVHYGYDDKGRLTGERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEW
                     LTYGSGYLAGMKLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQS
                     QHLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDD
                     ERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLS
                     RKPEVTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEK
                     LQQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRVWLAQCGLTVEQLARQVEP
                     EYTPARKVHFYHCDHRGLPLALISEDGNTVWSAEYDEWGNQLNEENPYYLYQPYRLPG
                     QQYDEESGLDYNRHRYYDPLQGRYITQDPIGLAGGWSLYAYPLNPVQHVDPLGLSTMI
                     IGNGPVPDNPFGHAAAANRYGLMSSGTGDEMGASVSDYFKKMQPRRDTWIWIIDTTDE
                     EEQCMMNKAIDLNKKLKTINLGPLPVANNCFSRTNMIFEACGFSTPSMNTNAPISLQV
                     LGELYGY"
     gene            complement(4224..6332)
                     /locus_tag="ACIE68_004346"
     CDS             complement(4224..6332)
                     /locus_tag="ACIE68_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFX5910554.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIALTGHPQANLNREWQVVASELHGEQPQAVPGR
                     SGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT         1262 a         2110 c         1793 g         1568 t
ORIGIN      
        1 gatatccata taactcaccg agcacttgta ggcttatagg cgcatttgta ttcatagagg
       61 gggtgctaaa cccgcacgcc tcaaaaatca tgttcgtccg gctaaaacaa ttatttgcaa
      121 caggaagagg cccgaggtta atagttttta gttttttatt aaggtctatt gccttattca
      181 tcatacattg ttcttcttca tctgttgtat ctattatcca aatccaggta tctctgcggg
      241 gttgcatctt tttaaaataa tcagagacag aggctcccat ctcatcccca gttcccgaac
      301 tcattaatcc atatcgattt gccgccgcag catgcccaaa aggattatca ggaacggggc
      361 cgttacctat tatcatagta ctaagcccta atggatcaac gtgttgcact ggattcagcg
      421 gatacgcata cagactccat ccccccgcca gcccaatcgg gtcctgggtg atataccgcc
      481 cctgcaacgg atcgtagtac cggtgacggt tataatccag ccccgactcc tcatcgtact
      541 gctgccctgg cagacggtat ggctgataca ggtaatacgg gttctcttca ttcagctggt
      601 tgccccattc atcgtattct gcactccaca cagtattgcc gtcttcgctg atgagcgcca
      661 gcggcaggcc ccggtggtcg cagtggtaaa aatgaacttt tcgcgccggt gtgtattccg
      721 gctccacctg tctggccagt tgctccaccg tcagcccgca ctgcgcaagc cacacccggc
      781 tttcactgct cacgcggtct gcccggattt cttcctccag tctgtccagc agccgcacca
      841 gttcagccgg aaataccacg ccgtgcccgt cctcgctccc ttcctgctgc agcttctccg
      901 ccaggctgcg gcgctgcgct ttctcccgct caccgttgtc tgtttcgatg cggatgagcg
      961 gcgcgaagct ccccggctga tataccgtct ggatacgcgt ggtgtcggtc tgtaccgtcg
     1021 tcagccggtc gccgtcccac ccgtaccacg tcacctccgg tttacgcgac agcgacatcc
     1081 accccgtcag gtcacgctcc cgccgccata cccgtttcgc cattcgccgt cccagcgggt
     1141 cgtagaggta gcggctctcg accagtggct cgccatgctg tatccgcgtg tagaacacca
     1201 gacggtgctg gctgtcgtag tggtagtggt gggtccgctc gtcgtccgtc cggataacac
     1261 ccgccgggat aaggtccgtc ttctccgtca gtctgccgta ttcatcatag tggtaaacat
     1321 agtgcgcatc ctctgcgatg cggttatccg gccacgccgt gagggtactg tccgggtgca
     1381 gctccgggtc cggcaggcgg tttcccgccg ggtcggtggc atacgggatg cggatgtcca
     1441 ggtccggtgc gagggtacgc acgctctcca gcctgcccgt ggcgctgtag ccgtattccc
     1501 gcgtctgtcg cgggccgctg atgcgcacca ggtcgccgtt gtcattccac ccgtagtcac
     1561 ggtcatacac caggctgttc aggtgctggc tctgtaactg gcctgcgggg gtgtatgtgc
     1621 tggtcagttt atatgcggca ttactgcctg ccatgctgcc aaagctgcgc accgtctccc
     1681 ggtgcatgcg gtcgcgcgtg tactccagca gcggcgtgtc gccaagcttc atgcccgcca
     1741 gataaccgct gccgtaggtc agccactcca ccggcggcag gctgtccggc gtgacgcggt
     1801 ttgccagccc ctgttcgttg taggcgtgtg ttgtctcatg ctgccacagc agttcccccg
     1861 tctccgggtt ctccaccgtc tgccgttcgc cggtcaggcg gcccttatcg tcgtagccgt
     1921 agtggacagc gacacggtgg ccttcgctca ggtggctgat gtctgtcagc cagccgtggt
     1981 catcatactg ccactgctct gccggttcgc cgttcaccgt gcggtgagtg atacggtccg
     2041 attcatcgta gtaccagagg gtgacaagcc cctcatcttc actctgcgtg agttttccgg
     2101 tcaggtcata atgataacgt tgcgtccgcc cgtcaaagcc gccctgctgt accaaccggt
     2161 ccagcgcatc gtagctgaag tcgctgtggc tgccgttctc gttagtcaga ctgatgacac
     2221 gtccggcagc atcgtactcc atactgcgcg tcagcccgcc ctgcgtggtg ctgaccgcct
     2281 ttccccacgc atcgtactgt gtctcgctcc ggttgccgtc cggggtgata acggcagtca
     2341 ggtcgcctgc ggcgttgtac tcatactgcg tttcacggcc ctgtgcgtct ttcaccgagg
     2401 ttaaccggcc acggttgtca tagtggcggt aaaggctgat accttcctcg cggtgaaccg
     2461 ccgtcatctg gccgaagcgg tcgtattcat agcgggtctg gtagcccgag cagtcagtga
     2521 acgccagcaa ctgcccgtag cggctccacg tcatctgccg ggtgctgccc gtcgcatccg
     2581 ttgtcgtcgc cggtaactca ctgtgcgcgt catcgtagcg gtagcgtact gtctccccgc
     2641 tgcgcgatgt ctccgatacc agcctgcccg gttcatcata ttcccggcgg ctctccagcc
     2701 cgtccgggga caccaccgcc gtcagctggt tcccgtcgtt atagtaaaat ttcgtctccc
     2761 gcccgtccgg cgtggtgatg tccgtgatat cgcctgacac cacattcaga ccgtactctg
     2821 tccgccgtcc cgccgcatcc gtctgcgccg tgagccttcc tgccgcgtca tacccgctgt
     2881 gcgtgacgct gccgtccgcc agttcttttt tcaccacccg cttcagcccg gccccgcctt
     2941 ctgtatgcag cacctcacgc cggttcaggc tgtccgtgac ggtgatgcga tcctgctcat
     3001 actggtagcg gtaactcagg cctgccgggt taagttgctc caccacccgc cccgcatcgt
     3061 cgtagcggta gcgcatctcc ggcctgcctg cataacggtg cgccaccatc cggcccgggt
     3121 gctgcgcgtc atacgtgaaa gcgcgcacct gcgtattgct gcggtcatat accgccagca
     3181 gttcaccggc ttccgtatat gtgtaccgca ccagtggcgc agcgggcagg ctctccgggt
     3241 atgccgggtc gtgcatcagc cacaccgccg aaaggcggat acccctgtcg gggccgtatt
     3301 cggtaccggg cagtgtgtcg gggaacgctg aggctgagag agggcgggaa ctgtcagaag
     3361 aagatagcga agaggtgcgg gcctcttccg cacgctgcgc ctgcgtggtc agcaccagac
     3421 ggaactcccg cccggcaccg tccgtcacgc cggtgatttc cccggtcagg tcaccggcgg
     3481 cctcacgccg gtacgtcagc gtccgcccga agcggtccgc cagccccgtc agctcccggt
     3541 acggcggcag cggcgctggc agcacgtcct cagcacccgg cacccgctct gaccagccga
     3601 gtatccacca cggcccctgt gcgctgttgg tcgccaggta aagatgcggg cttaaccgga
     3661 tatccggcgg cagcgccccc cacagccgcg ccagcgtatg gccgtccggc tgtgctgcct
     3721 taccaccgcg caccagccac attgactcac tgcggctgta caccgcctcc cccggcagca
     3781 gcggctcaaa gtgaatgctc cgcccgccgt tgtcgttgag tatcagtccg tcatcacgta
     3841 gctgtaagcg gatatcagaa ggcgctttcc agccggggcc gaaaacgccc accggtgcag
     3901 gcgtcttcgt ccggtagctg ctgtaggtgc gggagagaat gaacggcagc gggccgggca
     3961 gcgcaaggtc cgtctcgccg ggcagcacct tcgccccaag cagcggattt accgggttgc
     4021 ccgaagtcat cccgcccgga cacaccgagc acgccacgcc ggtgggcgcg ccaattctta
     4081 cacctgccga accctggaca atgggaccgc catactgagt catatctccc tgacgcgccg
     4141 ctggttttcc gctcatgatt caaattcctt cttctgttta tttttaatgc tggtttgtta
     4201 ctgaattccg aacaggtacg ggatcagtac tctctcatat ccggcgtatc ctgcttttca
     4261 tcaggggtct tcggtgggga tttcgaggct gcacagttcc agttaatcaa cagtgagtca
     4321 ccattaacct gctccttacc ctgcaaatga atttttgtgc cgttgagaaa aatttgtccg
     4381 tccttcgtca gcaccagcct tgccttacca cagcagagct ccagatgctc accggcggag
     4441 taaatcgcgg tctgcccggt atcatccttt ttcgttttac caacggtgaa ggtaacgtta
     4501 tttccgactt tgacgtcata acccagcccg accctcaacg atttatgcaa accgatctgc
     4561 gaggactgca tcagtgccac cgaggtgttc ataatgccac ctaccgtcgt ctgatacgcc
     4621 acgcccacgg tcagcgcacg aacaagtcca atcgtttcca cctgaacaga acccacttta
     4681 attatctggt tactgcccac cgtctctatc tggttaacac ccaccgtctg tatctgattg
     4741 ttggtaaccg caatcatctg attgttgcca atggtttcag catggttgtt tatcacatca
     4801 gtggtgcggt tattcagcac ctcggtgttc atgttcttct gcgcgtggat gtagacctgt
     4861 tctttccctg tcgcatcgtc aaacttcagt tcattaaacc cgctgccctt ataggttttc
     4921 gaacgaatgg tcatctgcgt cttcgtcccc ggcaggctgc cgggtgtgcg gttttcctgg
     4981 tggtaggtac gccccataat aatcggctgg tccgggtcgc cgttgaggaa gtccacaatc
     5041 acctcctgac ccactcgcgg tatcgccagg ttgccaaagc cggtgcctgc ccacgcctgt
     5101 gccacacgga tccagcatga actgtcctgg tttgacgggt tatagcggtc ccagttaaat
     5161 ttcacccgca cgcggccatg ttcatcacag aagatttcct cgcctgccgg tcccgtcacg
     5221 acggcgctct gcgggccgtc caccagcggt ttcagcaacg gctgtggccg ccatgtccgg
     5281 tctgccggta ttaccgcaaa gtggttattc agcgtggtac ctgaaccact gcgtcccggc
     5341 accgcctgcg gctgctcgcc gtgcagttca cttgccacca cctgccattc ccggttcagg
     5401 ttcgcctgcg gatgccccgt cagcgcaatt cgccgtcccg gccatatctc cggagaacgg
     5461 cttgttccgc gcgccacttc tgcgttgttt cgccagccat ccatctgcca gcgggcaaag
     5521 ttctgcccgt gggcaccctt gaaacgtccg gggtagtcat acacttcata ctgtgtgcgc
     5581 tggtaatcct ggtactggcc ttcctgatca aaacgccctg cccagccggg tcgtttaaag
     5641 gtgtagtcct tggtcaccac ggaagaaggg cggatttgtg cgctgtagcg gaactggctg
     5701 atgcagaggg tgctcacctc ggtacgggtg ttcggattcc aggggatctc aaaggactcc
     5761 ggcagataac gcacggtgtc gcacaggacc aggctctggt cggtactttt ttgcgcgtgc
     5821 tcctcataaa agaagatgcc ttcctccgcc gccatccggc acaggaaatc gtaatcggtt
     5881 tcgccgtact ggacacaaaa ctcacgggaa ggatgcggct cgctgaacag cggactccac
     5941 tcggtcacac cgttttcctt caggatcgta gcgaggatgc tttcgatgtc ctcattctgg
     6001 aagatacgga agttctggcg cagccctgtg cgccacagcg gcgggcacac cttcatgctg
     6061 tacagcatct ggtttttgtc gttctccccc agttcaaacc aggtcaccac acctttcacc
     6121 cggcgctgta cgtcatcgcc ctgccatacc gtcaggtagg ccattttgtc cagcacctgc
     6181 gcaaattcaa gggaaagaaa ctgctgactg accagagaaa ggtcgaggga aaaaagtgaa
     6241 gagagtgact gggtgagatg aaaggaaacc accgcaaacg cgtccggtgg caggccgtcc
     6301 acttcaagtg tgaaacgtaa tccggttgac atagcaacct ccgtgaaaat gaataataat
     6361 ccgcacatat tgcatgtaca acatgtcggg cggatgagat aagcgagtcg acatctggcg
     6421 ttaccgggga atatcagaaa aatgtcatgg ttatgtgatg atttagtctg ggattttact
     6481 tcagagaaac gggtgtctgt ttagtcaaca aaataatcac aaacgcaacg gatgtgttct
     6541 cgacgttatt cacgacaggc tcgtacatta cagcgagtaa taaaaaacct gatatgcaaa
     6601 gtcaaaccaa tattcataat gtctttttat tcacaacaat aaataattca ataacaatca
     6661 ttgaaatgaa tgatgatttg tataaggaat atacgctacc gccccaccct gataacatga
     6721 tctgcacatt gcc
//
LOCUS       DBANUU010000202         6692 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000202 DBANUU010000000
VERSION     DBANUU010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.205"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            162..890
                     /gene="gadW"
                     /locus_tag="ACIE68_004347"
     CDS             162..890
                     /gene="gadW"
                     /locus_tag="ACIE68_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFX5910555.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1253..4366)
                     /gene="mdtF"
                     /locus_tag="ACIE68_004348"
     CDS             complement(1253..4366)
                     /gene="mdtF"
                     /locus_tag="ACIE68_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFX5910556.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLIKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4391..5500)
                     /gene="mdtE"
                     /locus_tag="ACIE68_004349"
     CDS             complement(4391..5500)
                     /gene="mdtE"
                     /locus_tag="ACIE68_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFX5910557.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5608..5886
                     /locus_tag="ACIE68_004350"
     CDS             5608..5886
                     /locus_tag="ACIE68_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910558.1"
                     /translation="MRDLQTSGIVGLSASKVGYRYSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5887..6414)
                     /gene="gadE"
                     /locus_tag="ACIE68_004351"
     CDS             complement(5887..6414)
                     /gene="gadE"
                     /locus_tag="ACIE68_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFX5910559.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
BASE COUNT         1688 a         1619 c         1618 g         1767 t
ORIGIN      
        1 ctgcatttac ttattatcaa ttaactgtta tgcaaaacta ctttgtggat aaattttggt
       61 cctaccaaat ctggcagttt tttcgctaag aaacagtctg gcaacatttc attagtatac
      121 tgaaattgaa ataatcgcag tatgaaatat aagggataat catgactcat gtctgctcgg
      181 tgatcctcat tcgtcgttca ttcgatattt atcatgaaca gcataaaata tcgctgcata
      241 acgagagtat cgtgctgctg gagaaaaatt tggcagacga ttttgcgttt tgttcaccgg
      301 atacgcgacg actggatatc gatgagctga cagtttgcca ttacttacaa aatattcgtc
      361 agctaccacg caatttaggg ttacatagca aagaccgtct attaattaac cagtcacccc
      421 ccatgccgct ggtgacggcg atttttgata gcttcaatga atccggggta aattcaccga
      481 tactgagcaa tatgctctac ctttcctgtt tatcgatgtt ttctcataag aaagaactga
      541 tccccttact tttcaatagc atcagcactg tttcaggaaa agttgaacgc cttattagct
      601 ttgatatcgc caaacgttgg tatctgcgcg atatcgcgga aagaatgtat accagcgaga
      661 gtctcatcaa aaaaaagttg caggatgaaa atacctgttt cagtaaaata ttactcgctt
      721 ccaggatgtc gatggccaga cgattactcg agttacgtca aattcctctg catactattg
      781 cagaaaaatg tggctatagc agtacgtcgt actttataaa cacatttcga caatattatg
      841 gtgtaacgcc acatcagttt gcgcaacatt cgccaggtac cttttcctga catattttgc
      901 atttgaatat tggtcaggat ctcacacctg cttcatgtga aactcttccc tgatgatttc
      961 tgccgggcta ccggctagtt ctctttcgca gtttgccagg cggtataggt attttcagga
     1021 ggaaaagact cggcatgttt gggattatta agctgacaat tcatacgatt acgggaatgt
     1081 gggtcagtat tgtgctgttc aaattgatga cgaatggctg gagtggtttc tactttcagt
     1141 gctgtgtact aagcctggta ttcctgacgg ttagctggtt gttgagtggt gaatggttag
     1201 caggaaagag taaggctgaa ccttcacgtt caaccttact ctcatttaca cgttacgctt
     1261 ttttaaagcg ggcaaagaga tgttccacta caacgaaaaa gaccggaacg aagtaaattg
     1321 ccagcactgt tgcggcaaac atcccgccca tcacgccggt acctaccgcg ttttgcgcgc
     1381 cagaaccggc accatgactg ataaccagcg gcagcacgcc gagaataaag gccagagagg
     1441 tcatcaggat tgggcgtaaa cgcatccgcg ccgcttcgat gattgcctct atcggcgttt
     1501 tcccttcttt ctgcatcatc tcaacggcaa attcgacaat caggatggcg ttcttggcgg
     1561 aaagcccgat ggtggtcagc aaaccaacct ggaagtagac gtcattactt aagccgcgca
     1621 gatcggtggc cagtaatgcg ccaacgacgc ctaacggaac aaccaacatc accgagaacg
     1681 gaattgacca gctctcatag agtgcggcga gggcgaggaa caccacgacc agtgaaatgg
     1741 catacagtgc aggagcctgg tttgaggata acgcttcctg atacgacagt ccggtccatg
     1801 agtagccgac gcctgccgga agtttagcga ccaggtctgc cataaatttc atagcgtcac
     1861 cggtactttt cccggccgcc gcttcaccta aaatctccat tgacgggatg ccgttgtagc
     1921 gttccaatcg cggtgaacca taggtccatt cggtagacga gtaggcagaa agcggtgcca
     1981 tcgtgccaga ggcgttgcgt acataccatt ggttgatgtt atccggcaac atacggaacg
     2041 gcgtgcctgc ctggacatac acttttttca cccgcccctg gttgaggaag tcgttcacgt
     2101 agctgctgcc gaaggcggtg gaaattgtct ggttgatatc agacagcgcc acacccattg
     2161 cctcagcttt cgcggcgttg acgttcactt tgaacatcgg cgtatcttcc aggccgttcg
     2221 ggcgtacccc ggtgacctgg tcaggcgatt gcgctgccag tgataacagc tcgtttcgtg
     2281 cctgggttag tttttcgtgc cccaggttac cgttgtccag cagttccata tcaaaacctg
     2341 acgcggtacc cagttcagcc accgcgggta agttgaacgg gaagacgacg gctttattga
     2401 tactgcttaa cgcaatcatt gcccgctgaa tgatcgcggt aaccgagttt tcctcaccga
     2461 cacgttcaga ccacggcttg agactgataa acgccaggcc gttgttttgc ccctggccgc
     2521 tgaagccaaa gccgccaacg gtaaacaccg actgaacatt atctttctct tttataagat
     2581 aataatccgt cacctgttgc agcactttcg tggtgttaac catggtggca ccggaaggta
     2641 actgcgcggt ggtcataaat accccctgat cctcttctgg taagaaagag gtcggtgtac
     2701 gcaggaacag caccgccatc ccggcgcaaa tcagcaggta gaccaccatg tagcgaccgg
     2761 tacaacgcaa cagcgagcgg gtgctatcgg tatagtgctg agttgatttt tcaaacagcg
     2821 tgttgaagcg tgcgaacagg gcgttaggtt tgtgaccgcc ttccggcgcg gctttcagaa
     2881 tggtggcgca cagggcaggg gtcaggctca ttgccacaaa tactgaaagc agcatggagg
     2941 agatcagcgt gatggagaac tggcggtaga tctccccggt tgcgccgctc ataaaggcca
     3001 tcggcataaa cactgcggaa agaacaacag caataccgac cagcgcacgt tggatctgcc
     3061 ccatcgattt atgcgtcgct tccttcggtg gtagcttatc ttccgcaatg acacgctcga
     3121 cgttctccac caccacgatg gcgtcatcca ccagtagccc tatcgccagc accatcccga
     3181 acatagttaa cgtgttgatg gtgaaaccga ccgccgacaa gatcgcaaac gtcccgagaa
     3241 taaccaccgg tacggcaatc gtcgggatga ttgtggcacg gaaattctgc aaaaacagat
     3301 acatgaccag gaagacgagg atgatagcct caaccagtgt tttaaaaact tcctgaatag
     3361 aaatttcgat aaacggcgtg gtgtcgtaag gataaaccgt cttcagactt gcggggaaat
     3421 aggctgataa gcggttcagc tcctctttga ctgcccgcga ggtatccagg gcgtttgctc
     3481 cggtagccag tttgatggcg atcccggcag caggtttgcc gttatagcgc gccacggtgg
     3541 aataatcttc cgctccaagt tcgacgcggg cgacatcacg cagcagcact tgcgaaccat
     3601 cttgctgaac tttcaacagg attttgccaa attcttccgg cgtttgcaga cgcgtctgca
     3661 caatgatcga ggcgtttagt tgctggtctg ccgcctgtgg catgccaccc agttgaccac
     3721 cggaaatctg gttgttttgc accttaatct gggaaataac atcggaaggt accagattgt
     3781 atttattgag tttttgcggg tccagccaga tacgcatggc gtactcggaa ccaaagagct
     3841 gtacgctacc aacgcccgcg gtacggctta gcgggtcttt gatattagac gctacatagt
     3901 ccgcgatatc gtactggttg aggctgccgt tatcagaaat aaacgccgct accatcagaa
     3961 tattactgct cgacttatca acgctaatcc cctgctgctg caccgcttca ggtaatgaag
     4021 gcatagcgag ttgcagttta ttttgcacct gaacctgtgc gatatcagga gatgtcccag
     4081 tctcgaaggt cagagtgata gaggcattac ccgccgcatc actggttgaa gacatgtaca
     4141 tcaggccatc aagcccattc atattttgct caatcacctg agtgaccgag tcttctaccg
     4201 tttgcgcatc ggcacctgga taggtagcgc tgacggtaat ggtcggtggc gcaatctgcg
     4261 gatactgcgc aaccggtaag ttcatgatcg ccagaccacc tgcaagcatc ataataatgg
     4321 caagtaccca ggcaaaaacc gggcgatcaa taaaatagtt agccatgaaa gtccccttaa
     4381 gcctgcaacg ttattgtttc gattcggtgc tggcgttttc ctggctggag gaaattgctc
     4441 gtgctttgat acccggacga atgcgttgca aaccggaaac gatcacccga tcgccagcct
     4501 gcaagccaga ggtgacaacc cactggtcgc cgatggcttt gctggcttca atttcgcgta
     4561 gctgcacaac atcgtcttta tccagaatga gcgccgttgc tttaccctgg gcgttgtggg
     4621 tgacgccttc ctgcggcacc agtaatacat tctggcggct accttcatcc actaatgccg
     4681 tgacgtacat gccaggcagc aagtcaccat ttgggttggg gaaaatcgcc cgtaacgtca
     4741 cggagcccgt ggtttcatcc accgtcgggt cggagaattt cagcgtgccg gtctggctgt
     4801 agcgtttacc attttccaga ttgagctgta ctggcgtact gccctgaacc tgtttgattt
     4861 gcccactggc gacctcttct ttcatgcgta agaaatcttg caccgactgc gtgagatcga
     4921 cataaatcgg gtccagacgt tgtacggtaa ccagcgaatc tgcctgatta gcggtaacga
     4981 gtgcgccgac ggtcaccgac gatttcccgc tgacgcccgt aatcggcgag gtgacattcg
     5041 cgtattgcag attgatcgtc gcctgttcaa cagccgcttt ggcgacggtg acattggctt
     5101 ctgcttcatt caactgggtg cgcgcggtgt cgtaatcctg acggctaacg tagttggtct
     5161 tcagcaacga tgcctggcgg ttaaaggtga tgcgggcatt gctggcggta gagagcgctt
     5221 tcgccagcga gcctttggcg gagtttagct cggcctgtaa aggtgcagga tcaatctgat
     5281 acagcgaatc gccctggttc actttatcgc cttcgataaa gttgcgttta atgataatac
     5341 cgcccacctg gggacgtatc tcggcaactt cataaggaac ggttctaccg ggcaattcgc
     5401 tcaacacatt gaccgaaccg ggggagagtg tgacgacacc gacctcaggc gtcatggcgg
     5461 cggcgttttc cgccgatttg tcatcgcagg cggtgagcat cgcgccgcag aataacaacg
     5521 gtattaacag ctttcttctt ctgttcattt tagtccctga aaattcttga gaaataacgg
     5581 ttctttaaat tgcgcgcgag gagagatatg agggatttac agaccagcgg catagtgggg
     5641 ttgtcagcca gtaaggtggg atacaggtat agtgatcgac atggtgaggt caacgacaaa
     5701 aaaaatgttg ccgttctgcc aacagttcct gccagcattc gggcaacgaa gggttctact
     5761 ggtggataca cataccaggg gaataaagtc gttttagcct ctcctttagt aatcaccggg
     5821 ggtaatgaaa tcagcatctg catccctcgt catgccagcc atcaatttca gttgcttatg
     5881 tcctgactaa aaataagatg tgatacccag ggtgacgatg tcgctcatac gtcttagttt
     5941 cagcttgtac ataatgttga atttctgaat ctttaatgtt tgttgcgaaa tattgagcgt
     6001 cgacgtgata ttgctttcat ttttattttt cagaatcagt tgcataattt tgaactgatt
     6061 atgtgaaaaa cataattgtg aattcttatg gggcaagtgt ttaccataag taatctcaag
     6121 aagatttttg atactttctt tgcggctaac aaaataaata ggcgctttag cttttagttt
     6181 actgatgtga taaggtgcca aaacaataat tctctcggca tctaatttct ccagaaattt
     6241 aatcgcttct tcatcaagga tatgattgtg ataggtatca atgataacct tgaagggttt
     6301 attgcctact tttttgatct ctgacaggga attgattttt atcatttcgt gattatcttt
     6361 caactgccaa aagccctgta aaagaaaaga atctttcgtc atgagaaaaa tcataacttg
     6421 ctccttagcc gttatcgttt cgaacatgtc atccttttca ttatttacat ccttgtccga
     6481 atcgttgttc aatatagtaa acgcctataa gaactttgcc cttcgtgaac taatacgtct
     6541 attaacacta atcaatactt cagatttatc catcacgcac tccatatatt gatcctaagt
     6601 aagattcctg gttgttatca gtttgtaaac agtaaacaaa aagttaacgt ttgcggatta
     6661 ctgcccaaga ataagttatc aataaccagt tt
//
LOCUS       DBANUU010000203         6680 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000203 DBANUU010000000
VERSION     DBANUU010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.206"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            109..1242
                     /gene="wzz(fepE)"
                     /locus_tag="ACIE68_004352"
     CDS             109..1242
                     /gene="wzz(fepE)"
                     /locus_tag="ACIE68_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFX5910560.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISTLVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKAHVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGGVLLRYAMASRKQDAMMADHLV"
     gene            complement(1239..2054)
                     /gene="fepC"
                     /locus_tag="ACIE68_004353"
     CDS             complement(1239..2054)
                     /gene="fepC"
                     /locus_tag="ACIE68_004353"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFX5910561.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(2051..3043)
                     /gene="fepG"
                     /locus_tag="ACIE68_004354"
     CDS             complement(2051..3043)
                     /gene="fepG"
                     /locus_tag="ACIE68_004354"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFX5910562.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            complement(3040..4044)
                     /gene="fepD"
                     /locus_tag="ACIE68_004355"
     CDS             complement(3040..4044)
                     /gene="fepD"
                     /locus_tag="ACIE68_004355"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFX5910563.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLIAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            4155..5411
                     /gene="entS"
                     /locus_tag="ACIE68_004356"
     CDS             4155..5411
                     /gene="entS"
                     /locus_tag="ACIE68_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFX5910564.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPPPQPREHPLKSLLAG
                     FRFLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIG
                     ALTSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQ
                     YTMLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLII
                     GVLLLLVLVELRRFRQTPPQVTASDS"
     gene            complement(5415..6371)
                     /gene="fepB"
                     /locus_tag="ACIE68_004357"
     CDS             complement(5415..6371)
                     /gene="fepB"
                     /locus_tag="ACIE68_004357"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFX5910565.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
BASE COUNT         1538 a         1781 c         1857 g         1504 t
ORIGIN      
        1 attcagtgga aggaatttac aaacttcagc ttcggataat tatcaccaac tggtaaagtg
       61 agcgccgtgg agatttcccc tattggactc attttcaggt tataccccat gtcatcactg
      121 aatattaaac agggaagtga cgctcatttt cctgagtatc ctctggcgtc gcccagtaat
      181 aatgaaattg atttacttaa tctaatcgca gttttatggc gggccaaaaa aacggtcatg
      241 gcggtcattt ttgcgtttgc ctgcgcaggc ttgctgatct ctttcatcct gccgcaaaaa
      301 tggaccagcg cggcggttgt cacgcctcca gaacctgttc agtggcaaga gctggagaaa
      361 acattcacta agcttcgagt gctggatctg gatatcaaaa ttgatcgtac agaagcattt
      421 aacctgttta tcaagaagtt tcagtcggtt agcttgctgg aagagtacct gcgttcatca
      481 ccttatgtga tggaccaatt aaaagaggcg aaaatcgacg aactggattt gcatcgcgca
      541 attgtcgcat tgagcgaaaa aatgaaagcg gttgatgaca atgccagtaa gaaaaaagat
      601 gaaccgtcac tgtatacctc ctggacgcta agttttaccg cgccaaccag tgaagaggcg
      661 cagaccgttt tgagcgggta tatcgattat atctctacgt tggtggtgaa agagtcgcta
      721 gaaaacgtcc gtaataaact ggagatcaaa acccagtttg aaaaagaaaa actggctcag
      781 gatcgcatta aaacgaaaaa tcaacttgat gcaaacattc agcgcctcaa ttattcactc
      841 gacattgcca acgcggcagg aattaaaaag cccgtttaca gtaatggtca ggccgttaaa
      901 gatgaccccg atttttctat ttctctcggt gcagacggta ttgaacgcaa actggaaata
      961 gaaaaagcgg tcactgacgt tgcggaactg aacggtgaat tacgtaatcg gcagtatctt
     1021 gtcgagcaat taacaaaagc acatgtcaac gatgtgaatt ttacgccgtt taaatatcag
     1081 ttaagcccgt cattgccagt gaaaaaagac ggtccgggta aggcgattat tgtgatcctt
     1141 tccgcgttga tcggcgggat ggtggcttgt ggtggcgtgc tgttgcgcta tgcgatggca
     1201 tccagaaaac aggatgccat gatggcagac cacttagttt aactatttgc ggttgaaggt
     1261 gccgttcgtc cgagcggcac cacaagcggc gttccggcaa ctggatcgtc aatgatcatg
     1321 cagcgcagac cataaatgcg ctcaatcagt tcagcagtga caatctcctt cggcgctccc
     1381 tgagcaacaa ttttcccttc ccgcaatgca atcaaatggc tggcgtaacg acaggcctga
     1441 ttaagatcgt gcagcaccgc cgccagggta tagccttttt cgcggttcag ttcgcttaac
     1501 aactccagca aatcaatctg atgactgata tccagccagg tcgtgggttc gtcgagcagc
     1561 ataattgccg tttcctgggc cagcaccatc gcgatccacg ctcgctggcg ttgtccgcca
     1621 gaaagggtat ccacgctttg atctgccaga tgagttattc ccgtggcctg cattgctttc
     1681 gttaccgctt cttcatcctc tttgcgccag cgggtaaaca gcggttgatg cggataacgt
     1741 ccacgcgcca ccagctcctg cacggtgata tcgcccggcg tggtagcatt ttgcgccaac
     1801 agaccaatcc ggcgtgcaac ctctttactg gcgtaatgtt gaatgtgctc gccatccagc
     1861 cagacatgcc catgagcagg cgtcatcagg cggcttaagg tacgcagtaa cgtggattta
     1921 ccgcagccat ttggcccgat aattgccgtg aagtgaccat caggtatttc tacagtcaga
     1981 ttttccgcaa cggtatattt gccatatccc agggttaact gttcgccgcg caaacgggct
     2041 actgattcgg tcattttttg cgagactcct gaattaacaa gacgataagg taaataccgc
     2101 cgaggctgac ggtaacgacg ccaaccggaa gttgatacgg cataaacagt tgttgggcgc
     2161 agagatcggc cgccagcagt aacagcgccc cgcatagcgc cgcctgggtt agcccccagc
     2221 gagcggtgcc gctaatgcgt cgggcaatgt gcggtgcgac taaagcaata aaggaaatcg
     2281 gcccggcaag cgctgttgcc gcagcggtaa gcaccactgc aaccagcatc attaacagac
     2341 gcgaacgttc gacgctgacg cccagcgcac acgcggtatc atcgcccatt tccagcaagc
     2401 gcatccggcg taccagtaag gcggcggcaa tgagcatcaa tataatgatg ggtgcggaag
     2461 gcgaggtttt tgcccacgtc aggccgttga gcgatcctgc attccacaaa cccgctgtta
     2521 gcgccgtttc taaagacgct ttcagcaaca gccaggtatt aaaggccacc agcatggcac
     2581 gaacgccgat accgataata atcaaccgaa aggtgtcgat gccgttacgc caggcgagca
     2641 gccagaccaa cagcgaagtg ataatgccgc ccaccattgc tgccagcgcg atagccgtca
     2701 ggtcctgacc aaacagcacc atcgctacca gcacgccgct ccacgccccg gtgttaaagc
     2761 ccattacgtc agggctgccg agcgggttac gcatcagcga ctgaaaaatc gcgccgctga
     2821 cgcccagtgc cgcgccaatc aacagcgcca tcagcacgcg tggtaaacgc cattcggtga
     2881 ccaccatcgt catactgcgc ggcgcatcgc ccatcagcgc ggcgaatacc tgcgaggttt
     2941 ccagcgtgac ggcaccgctg cgtaatcccc agatacctgc aaccacacag gcggaaacca
     3001 acagcaaaca ggtgatgatt aatcggcgag agacgtaaat catgcgccac ctcgcgtttt
     3061 acgtcgcacg aggaagatca gcaccggtgc accaataaac gcactgacca cagaaacgcg
     3121 cagttcgccg ggcacaatca cccgcccgat gatatcggca aacagcagca gggcaggggt
     3181 agcaagtagc gtgacgggca gcgaccagcg atgatcggca cctaccagcc agcgcgccat
     3241 gtgcggcatc atcaggccga taaaggcaat cgggccaact accgccgtcg cactaccaca
     3301 aagcacggta atcgccagca gaccaattaa ctgtgtgcgc gccacgcgac tgcccagcgc
     3361 cgtcgcggtg tcgctgccga ggcttaaact gttcagcgcg cggctcagta atagcgcggt
     3421 tgctccggcg atcagcaccg ggatcagcac cacttttaag gtatgtagat tgcgaatatc
     3481 cagcgaaccg gcttgccaga aacgcagttg atcgtagaca tcaggattaa gcagggcgat
     3541 gccgctggtc agcccttcca gcaccgccgc cagcgccacg cccgccaggg ttaaacgcac
     3601 cggacttaac tgcccgccgc cctgactgcc ggtaaaggca acaatcaatg aggccaccag
     3661 cgccccggcg aaggccatcg ccagttgttc ctgcgcggat gagtaaccaa acagcgccgc
     3721 acccagcaca atggcaaagc tggctccggc attcacgcca agcaagccgg ggtcggcaag
     3781 tggatttcgg gtgagggttt gcatcaacgc cccggcaagg ccaagcgcgc cgcctgcaat
     3841 taaaccggca agggtgcgcg gcagtcgcgc gtcgagcacg atggtgcagt cggcgctctg
     3901 gcaggtgccg gagagggcct ccagcactac ggaagcgggg agtgattttg ccccaatgag
     3961 caggcttaat gccgtcgcga taatcagtaa taacagcaat ccgggcacgg caatggcgcg
     4021 tgtcacggca acagaaccag acatacgaac ttccatgata atgaaattaa ttatcgttat
     4081 cgatcttatt tggatatgtt agcatgtgca gcctaagaat aggtatttaa aatatttgat
     4141 ggcaaggcat tgtaatgaat aaacaatcct ggctgcttaa cctcagcctg ttgaaaacgc
     4201 acccggcgtt tcgcgcagta ttcctcgctc gttttatctc aattgtgtct ctgggtttgc
     4261 tcggcgtcgc ggtgccggtg cagatccaga tgatgacgca ttctacctgg caggtggggc
     4321 tttcggtgac gctgaccggc ggcgcgatgt ttgttggcct gatggttggc ggtgtgctgg
     4381 cggatcgcta tgagcgcaaa aaagtgattt tgctggcgcg cggcacctgt ggcattggct
     4441 tcattggact gtgccttaat gcactgctgc cggagccgtc attgctggca atctatttac
     4501 ttggtttatg ggatggtttt ttcgcatcgc ttggcgttac ggcgctattg gcggcgacac
     4561 cagcactggt agggcgtgaa aacttaatgc aggccggggc gatcaccatg ttgaccgtgc
     4621 gtctggggtc ggtgatttcg cccatgattg gcggtttatt gctggcgacc ggtggcgtag
     4681 cctggaacta cgggctggcg gcggcgggca cgtttattac cttgctaccg ttgttaagcc
     4741 ttccggcgtt gccaccgcca ccgccaccgc agccgcgtga gcatccgttg aaatcattac
     4801 tggcaggatt tcgttttctg cttgccagcc cgctggtggg cgggattgcg ctgctgggtg
     4861 gtttattgac gatggcgagc gcggtgcggg tactgtatcc ggcgctggct gacaactggc
     4921 agatgtcagc ggcacagatt ggttttctct acgcggcgat cccgctcggc gcggctattg
     4981 gtgcgttaac cagcgggaag ctggcacata gtgcgcgacc agggttattg atgctgctct
     5041 ccacgctggg atcgttcctc gccattggtc tgtttggcct gatgccgatg tggattttag
     5101 gcgtggtttg tctggcgctg ttcggctggc tgagcgcggt tagttcgttg ctgcaatata
     5161 caatgctgca aacgcaaacc ccggaagcga tgttagggcg gattaacggt ttgtggacgg
     5221 cgcagaacgt gacgggcgat gccataggcg cggcgctgct gggtggtttg ggcgcgatga
     5281 tgacaccggt tgcttccgca agcgcaagcg gttttggttt gttgattatc ggcgtgttgt
     5341 tattgctggt gctggtggag ttgcgacgtt ttcgccagac gccgccgcag gtgacagcgt
     5401 ctgacagtta atgcttaaaa cagcgcctta agtcgttcca gcacttgcgt ggcgctgtag
     5461 taatccagac ggaacgtctc ggttcccagc gcataaacct gcttgttttg tactgcggga
     5521 aggtgcgcga gcagtggatt ggcataaatc gcatcggcat ctttctgatc accggcgaac
     5581 aggaatagtg actcgccatt taacccagca gccagatttt ccccaccaag ctgaatgatg
     5641 tcatggcgtt tgccctggct ttggctggcg tttaaccctg cgggcaactt cgccagcgta
     5701 aagccgagtt gttccagcat ctgcccttgt gctgattccg gcgtccagag attggcactg
     5761 tgtgcagcgg cggtatagac aatggcagtg accggctgcg gcggtaattt gatttgctct
     5821 ttcgccgctg ccagttgctt atcaaactgt gcaatccgct ctgccgcttg tttctcatgc
     5881 ccggtaattt cgccaagctg cgttaacagc gactgccagc ttttgtcgtc gtaattgatg
     5941 attaatgtcg gggcgatggt ggaaagctga tcatacagtg ccagcgccga atccccgccg
     6001 gttgcgctaa ttaaaatcag atccggcatt tgcgcggcaa cggcttcggc gctcggttcg
     6061 ccgatataga gccgttgcag tttgcgttct ttcgccacct tgctccactg gcgtaaaaag
     6121 ccctggtcat ccgcgacgcg gttattcggc gtggtcgcgc cgctggcgat caccggagca
     6181 tcaatcgcca gcagtgagcc ggtcagggtg acgctggtgg aaacaatacg ctgcggctgg
     6241 ctttccagtg tatgtgtgcc acggctgtca gtaatctgac gcggccagtc agcggcctga
     6301 actgcggcta ttcctgaaag caaaagtcct gttaatagaa gggcgttgcg gtagagcggg
     6361 gcgagtctca caaatcagct tcctgttatt aataaggtta agggcgtaat gacaaattcg
     6421 acaaagcgca caatccgtcc cctcgcccct ttggggagag ggttagggtg aggggaacag
     6481 ccagcactgg tgcgaacatt aaccctcacc ccagccctca ccctggaagg gagagggggc
     6541 agaacggcgc aggacatcac attgcgctta tgcgaatcca tcaataatgc ttctcatttt
     6601 cattgtaacc acaaccagat gcaaccccga gttgcagatt gcgttacctc aagagttgac
     6661 atagtgcgcg tttgctttta
//
LOCUS       DBANUU010000204         6469 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000204 DBANUU010000000
VERSION     DBANUU010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.207"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            181..852
                     /locus_tag="ACIE68_004358"
     CDS             181..852
                     /locus_tag="ACIE68_004358"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFX5910566.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDYLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            862..2058
                     /gene="yaiP"
                     /locus_tag="ACIE68_004359"
     CDS             862..2058
                     /gene="yaiP"
                     /locus_tag="ACIE68_004359"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFX5910567.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYSQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2036..2617
                     /locus_tag="ACIE68_004360"
     CDS             2036..2617
                     /locus_tag="ACIE68_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFX5910568.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATRCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2619..3392
                     /gene="yaiO"
                     /locus_tag="ACIE68_004361"
     CDS             2619..3392
                     /gene="yaiO"
                     /locus_tag="ACIE68_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFX5910569.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3577..3852
                     /gene="frmR"
                     /locus_tag="ACIE68_004362"
     CDS             3577..3852
                     /gene="frmR"
                     /locus_tag="ACIE68_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFX5910570.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3887..4996
                     /gene="frmA"
                     /locus_tag="ACIE68_004363"
     CDS             3887..4996
                     /gene="frmA"
                     /locus_tag="ACIE68_004363"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFX5910571.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5089..5922
                     /gene="fghA"
                     /locus_tag="ACIE68_004364"
     CDS             5089..5922
                     /gene="fghA"
                     /locus_tag="ACIE68_004364"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFX5910572.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6047..6424)
                     /locus_tag="ACIE68_004365"
                     /pseudo
     CDS             complement(6047..6424)
                     /locus_tag="ACIE68_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
BASE COUNT         1598 a         1544 c         1617 g         1710 t
ORIGIN      
        1 acttaacata tgaattgcat agaaacatta cccagtcgaa cctccgggtc tgaaagccct
       61 tgcttttcag ttattagcaa catttaacat ttataaatta acatctggct gcgccagaga
      121 ttacgtctct gagtgcatcg ttgtgcttgt agtataataa ataaaatgga gtgtcattaa
      181 gtggataagg ttttagattc agccctcctt tcttcggcaa ataaaagaaa gggtatcctt
      241 gctattggcg cacatcctga cgatatagaa ttaggctgtg gcgcatcgct tgctcgtctt
      301 gcgcaaaaag gaatttatat cgcagccgtg gtgatgacta ccggcaactc tggcacagat
      361 ggaataatcg atcgccatga agaatcgcgc aacgccctaa agatattagg gtgccaccaa
      421 actattcatc ttaattttgc tgacacccgc gctcatttac agctcaatga tatgatttcc
      481 gccctggaag acatcattaa aaatcaaatt ccttctgatg ttgaaatcat gcgggtatat
      541 accatgcatg atgccgaccg ccatcaggat tatctcgctg tttatcaagc ttcaatggtt
      601 gcctgccgca ctattccaca aattctcggc tacgaaaccc caagtacctg gctttcattt
      661 atgcctcagg tttttgaatc cgttaaagaa gaatatttca cagtcaaact tgctgcatta
      721 aaaaaacata aaagccagga acgacgcgat tatatgcgtc atgatcgcct gcgtgcagtt
      781 gcacaatttc gcgggcaaca ggtcaatagc gatctgggtg aaggctttgt aattcataaa
      841 atgattcttt gagcaaattg catgaaaacc tggatattta tctgtatgtc catagcaatg
      901 ttgctatggt ttttaagtac gctaagacgt aaacccagtc aaaagaaagg ctgtattgac
      961 gccattatac ctgcgtataa cgaaggcccg tgtctggcgc agtcactgga taatctactg
     1021 cgtaaccctt atttttgccg ggtaatttgc gttaacgacg gctccacgga caataccgaa
     1081 gcggtcatgg cggaagtcaa acgcaaatgg ggcgaccgct ttgttgccgt cacgcaaaaa
     1141 aataccggta aaggtggtgc gctgatgaat ggcctcaatt atgccacctg cgaccaggtt
     1201 tttttaagtg atgccgacac ctatgttccg cccgatcaag acggaatggg ctatatgctg
     1261 gcagaaattg agcgcggtgc tgatgccgta ggcggcattc cctctactgc gttgaaaggc
     1321 gcggggctgt taccgcacat ccgcgcgacc gtaaagttgc cgatgattgt tatgaagcgc
     1381 acgctacagc agctcctggg cggcgcaccg tttattatca gcggtgcctg cggaatgttc
     1441 cgcactgatg tattgcgtaa gttcggtttc tcagatcgta ctaaagtcga agaccttgat
     1501 ctcacctgga cattagtggc aaacggctac cgtattcggc aggcgaatcg ctgcatcgta
     1561 tactcacagg aatgcaacag cccgcgtgag gagtggcgtc gctggcggcg ttggattgtg
     1621 ggatacgcgg tctgtatgcg cctgcataaa agacttttat ttagccgctt cggtatcttc
     1681 agtatatttc ctatgctgtt ggttgtgatt tatggcgttg ggatttatct cactacctgg
     1741 tttaatgaat tcatcaccac cgggccgcat ggtgtggtgt tggcaatgtt tccgcttatc
     1801 tggatcggcg tagtttgtgt tattggtgct tttagcgcct ggtttcatcg ttgctggttg
     1861 ttggtgcctt tagcgccgct ttccgttgtg tatgtattat tagcttatgc catctggatt
     1921 atttatggac ttattgcctt ttttactgga cgcgaacctc agcgcgacaa acccacccgc
     1981 tattccgcac tggtggaagc gtcaaccgct tattcccaac cttctgtcac aggaactgaa
     2041 aaactatctg aagcttaatg ttaaagagaa aaatattcag attgcagacc aggtaattat
     2101 tgatgaaact gcaggtgaag ttgttatcgg cgcgaatacc cgtatttgtc atggtgccgt
     2161 tattcagggt ccggtagtga ttggcgcaaa ctgcctgata ggtaattatg cgtttattcg
     2221 tcctggcaca ataatcagca atggtgtaaa aattggtttt gccaccgaaa ttaaaaatgc
     2281 ggttattgaa gcggaagcaa cgattggtcc gcaatgtttt attgccgact cggtagttgc
     2341 aaaccaggca tatttgggcg cacaagtacg taccagtaat catcgtctgg atgaacagcc
     2401 tgtgtctgtt cgaactccag agggaattat cgctaccaga tgcgataaat taggttgtta
     2461 tatcgggcag cgttcacgcc ttggcgtaca agttataatt ttgcctgggc gaattatttc
     2521 cccgaacacg caacttggcc cgcgcgtgat tgtagaacgt aatttacctg ccggaactta
     2581 ttcactccga caagaactta tccgtacagg agattaaaat gataaaacgg acgttattag
     2641 cggcggccat ttttagcgca ttgcccgctt atgccgggtt aacttccatt accgcgggct
     2701 acgattttac cgattattct ggcgatcatg gcaaccgtaa tttagcgtat gctgaactgg
     2761 tggcgaaagt tgaaaacgca acgctgcttt ttaatctttc ccaggggcgt cgtgattatg
     2821 aaactgaaca tttcaatgcc actcgcggtc agggtgcggt ctggtataaa tggaataact
     2881 ggctgacaac ccgaacgggt attgcctttg cggataatac gccggtcttt gcccgccagg
     2941 attttcgtca ggatattaac ctggccctgt tgccaaaaac gcttttcacg accggttatc
     3001 gctacactaa atattacgat gatgtcgaag tcgatgcctg gcaaggcggc gtatcactct
     3061 atactggccc ggtgatcacc agctaccgct atacccatta tgactccagc gatgcaggtg
     3121 gtagttatag caatatgatt tccgtgcgtc tgaatgaccc gcgcggcact ggttatacgc
     3181 aactatggct aagccgcgga acaggcgctt acacctatga ctggacgcca gaaacacgct
     3241 acggcagcat gaagagcgtc agtttgcaac gtattcaacc gctgactgag caacttaatc
     3301 tcgggctgac ggcaggtaaa gtgtggtacg acaccccaac cgatgattat aacggtctgc
     3361 aacttgcagc ccatctgacc tggaaattct gattccttct gccgcccgct atccggggcg
     3421 gccttccctg ccgattagcc ccccctttcc tctttgtttt ccgaccacat tcaccggata
     3481 aattttattc tccagtgtta tatactatag gggggtatgc attgacatat agcatacccc
     3541 cctatagtat attgcgtgca gatgatgagg tgcgaaatgc ccagtactcc ggaagagaag
     3601 aaaaaggtcc ttactcgagt tcgtcgtatt cgagggcaga ttgatgctct ggaacggtcg
     3661 ctggagggtg atgccgaatg ccgtgccata ctccaacaga tcgctgccgt tcggggcgcg
     3721 gctaacgggc tgatggcaga agtgcttgaa agccatatcc gggaaacgtt tgaccgaaat
     3781 gactgctaca gccgcgaagt cagccaatcc gttgacgaca ctattgaact ggttcgagcc
     3841 tatcttaaat agctgaatct attaccatat tgaggaagag cgagagatga aatcacgtgc
     3901 tgccgttgca tttgctcccg gtaaaccgct ggaaatcgtt gaaattgacg ttgcaccacc
     3961 gaaaaaaggt gaagtgctga ttaaagtcac ccataccggc gtttgccata ccgacgcatt
     4021 taccctctcc ggggatgacc cggaaggtgt attcccggtg gttctcggtc acgaaggggc
     4081 cggcgttgtg gttgaagtcg gtgaaggcgt aaccagcgtc aaacctggcg accatgtgat
     4141 cccgctttac accgcggagt gcggcgagtg tgagttctgt cgttctggca aaactaacct
     4201 ctgtgttgcg gttcgcgaaa cccagggtaa aggcttgatg ccagacggca ccacccgttt
     4261 ttcttacaac gggcagccgc tttatcacta catggggtgc tctacattca gtgaatacac
     4321 cgtggtcgcg gaagtgtctc tggccaaaat taatccagaa gcaaaccatg aacacgtctg
     4381 tctgctgggc tgtggcgtga ccaccggtat tggcgcggtg cacaacacag ctaaagtcca
     4441 gccaggtgat tctgttgccg tgtttggtct tggcgcgatt ggtctggcag tggttcaggg
     4501 cgcgcgtcag gcgaaagcgg gacggattat cgctatcgat accaacccga agaaattcga
     4561 tctggcgcgt cgcttcggtg ctaccgactg cattaacccg aatgactacg acaaaccgat
     4621 taaagatgtc ctgctggata tcaacaaatg gggtatcgac catacctttg aatgcatcgg
     4681 taacgtcaac gtgatgcgtg cggcgctgga aagtgcgcac cgcggctggg gtcagtcggt
     4741 gatcatcggg gtggcaggtt ctggtcagga aatctccacc cgtccattcc agttggtcac
     4801 tggtcgcgta tggaaaggtt ccgcgtttgg cggcgtgaaa ggtcgttccc agttaccggg
     4861 tatggttgaa gatgcgatga aaggtgatat cgatctggaa ccgtttgtca cgcataccat
     4921 gagccttgat gaaattaatg acgccttcga cctgatgcat gaaggcaaat ccattcgaac
     4981 cgtaattcgt tactgatttc ccgcaggtat accccgtcca cttcagacgg ggttcttaat
     5041 actctccccg ggcagccgtc cgggggatta accgtgagat aatgactgat ggaactcatt
     5101 gaaaaacatg ccagctttgg cggctggcaa aatgtgtatc ggcattattc ccaatcactg
     5161 aaatgtgaaa tgaatgtcgg cgtctatctc ccaccaaaag ccgcgaatga aaaattgccg
     5221 gtgctgtact ggctttcggg cctgacctgc aacgagcaga atttcattac taaatcgggg
     5281 atgcagcgtt acgcggctga gcacaacatt attgttgttg cgccggacac cagtccgcga
     5341 ggcagtcatg tcgcagatgc cgaccgttac gatctcgggc aaggtgccgg gttttacctg
     5401 aacgcaacgc aagcgccgtg gaatgaacat tacaaaatgt atgactatat ccgcaacgag
     5461 ctgccggatt tagtgatgca acattttccg gcaacgacca gaaagtctat ctctggtcat
     5521 tctatgggcg ggctgggcgc gctggtgctg gcgttacgta acccagatga atatgtcagc
     5581 gtctcggcgt tttcgcccat tgtctcccca tcgcaagtac cgtggggaca gcaagccttt
     5641 gccgcatatc ttggtgaaaa taaagatgcc tggttggatt acgatccggt gagtcttatt
     5701 tcacaaggtc aacgcgttgc ggaaatcatg gttgatcagg ggttgagtga tgatttttac
     5761 gcggaacagc tgcggactcc aaatcttgaa aagatctgcc aggagatgaa tatcaagacg
     5821 ttaatccgtt atcacgaggg ttacgaccac agctattatt ttgtctccag ttttattggc
     5881 gagcatattg cctaccacgc caataaactg aatatgcgtt gataatagtg cacgactgcc
     5941 ggatgcggcg tgaacgcctt atccgcctac atttcgcccg caggccgtag gcatgataag
     6001 acgcgcatag cgtcgcatca ggcatctgcg cacgacttat ctatgtttac cacatcaaat
     6061 catcaggcac tttgaagtcg gcatacggat cgtcttcatc ctgctcttcg gcactgagcg
     6121 cgctgtgcaa cactatactg ctggcatcac gctgagctat tttatcggca acgctggcgg
     6181 gaataatggc gtattcacct tcgctattgt tatcgaccaa caagcgagca atcgccagac
     6241 gaccattaat caactgcgcc tgtgtgagct tatcgacaaa aatcttctta atcagattgc
     6301 cgtcagtgaa gttaaaacca atatcgccat tggcaatggt gattcggttc atttcaatga
     6361 gctgcttcac ctgagcttta tattctttcg ccaacgcagc ttgtttttgt tgctcgctaa
     6421 gctctgatga atcccctaat gatttttatc aaaatcatta agttaaggt
//
LOCUS       DBANUU010000205         6451 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000205 DBANUU010000000
VERSION     DBANUU010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.208"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(474..704)
                     /locus_tag="ACIE68_004366"
     CDS             complement(474..704)
                     /locus_tag="ACIE68_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910573.1"
                     /translation="MSYPTNVVALVESDFLAQAREMMKDREQAFNLYEWAIKCLHLGE
                     HRELVEQLLGELINEVFALNVQLHGRENNQSQ"
     gene            complement(890..1483)
                     /locus_tag="ACIE68_004367"
     CDS             complement(890..1483)
                     /locus_tag="ACIE68_004367"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFX5910574.1"
                     /translation="MIAAEKIKQRKRDNSLRDLWRTPDWLFSAIQRYLGVTFDVDVAC
                     NKDNAKLPNFIGVERDALKSEWGQPGTIAFLNPPYSKISPWIDAAIREQARGVTTVML
                     IPQSLDTKWYERATECANETIILSGGRVAFVEPDVNLGQVEVNINPGGSMLVVFRGFC
                     QDAGHSISKIPLDVMKSLGGYDPANVIRKKRPSKKAA"
     gene            complement(1667..2476)
                     /locus_tag="ACIE68_004368"
     CDS             complement(1667..2476)
                     /locus_tag="ACIE68_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910575.1"
                     /translation="MSSVENVMTNDDLDELAAMLQSLDEPVKKAAQVENTDDIDDLLL
                     GLDAGVAMSSDDVAEELFNEEKAGDFSSALNELELAHEPINVINAESGEAAENEPEQL
                     GIIEVEGCVEINDELKVQQSNDSNTNKKARTARGPRFALSDKDDAFFNKAGLEKDIFL
                     DAYDNAPVKAKDKILNLLNWFSGGPDISVYTVISMRHLLTEKKATSNSIKIALMSNPE
                     KPYPLNTASTQAGQMMAVFPATGIAVRDGGNLTLNEESPIVKKFVAEYTIG"
     gene            complement(2635..3192)
                     /locus_tag="ACIE68_004369"
     CDS             complement(2635..3192)
                     /locus_tag="ACIE68_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFX5910576.1"
                     /translation="MTTIVAGIDIESTGLDFLAGHKIIEIAITRYELETQRHIDSLEM
                     RFNPRRNIDPKAQAVHGISLEQLAAEPLLSNHASEIGAYMGACSVWVAHNGEAFDIPF
                     IRHEFSGYGVRLPEVPVIDTMLSGLWATEDGKRPRLEELAFSLGFIYDHAKAHSALYD
                     TNLMMQCFFKARNKYGFFKLPSEIV"
     gene            complement(3202..3621)
                     /locus_tag="ACIE68_004370"
     CDS             complement(3202..3621)
                     /locus_tag="ACIE68_004370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910577.1"
                     /translation="MFSEMLLEDELDRKTTEALIRVADEHSRSLMSDREARLAIRAIF
                     ETAQGLVGTQVGEAINIAMSQFSEGSKKPLFPMHLMLAGGTVLYISVCLDSNQINILN
                     TVSGKWKDPIVCETSEETLKKAAQFVRSALLKGAKKL"
     gene            complement(3683..4327)
                     /locus_tag="ACIE68_004371"
     CDS             complement(3683..4327)
                     /locus_tag="ACIE68_004371"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFX5910578.1"
                     /translation="MDYRIGITGAQGSGKTTLAKYIDKHYGIPYVDAGVGSLMSRLGV
                     RVGDSMPLYERLQIQMEIAKHIELLTRGVEGFVIDRTPADVMAYTLDLVGHTNEDRCI
                     ELALDIEKFCHKTAISNFNAIAGLRPGVALSERDYLRSQRASLDRLYVARIDALMCGE
                     LTKIHLHPQRGDLQTFVVSNRYRTVEARARSVMRMLDNAVEKIENRFCGRVTVH"
     gene            complement(4327..4803)
                     /locus_tag="ACIE68_004372"
     CDS             complement(4327..4803)
                     /locus_tag="ACIE68_004372"
                     /inference="COORDINATES: protein motif:HMM:NF047324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFX5910579.1"
                     /translation="MKMIAAVGRNYEIGIANELPWRCSTDLKLFKRLTKNATVVMGRK
                     TMESLKRPLPERHNLVLTRSHGFVPNGFYPAGVDDVLRLPEPVWVIGGEQIYSLFMPH
                     VEEIWLSHIGVDVPNADAFFPASMMRNLGFVPVETAYTQRASEEEPGFSQIVYRRS"
     gene            complement(4800..5213)
                     /locus_tag="ACIE68_004373"
     CDS             complement(4800..5213)
                     /locus_tag="ACIE68_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910580.1"
                     /translation="MARGMYVLCEIEGVLANASHRKSVSDADAGQLIAGDELIFPTSR
                     MLRGFARSGAEVVLISSRSETLEAPTKRWLKDFGVDYDWLHLVPNGTSYEKHIKRTLA
                     EHKGDLLIAALVHDPRLRAALADSHHRPVIYEVSK"
     gene            complement(5215..6318)
                     /locus_tag="ACIE68_004374"
     CDS             complement(5215..6318)
                     /locus_tag="ACIE68_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFX5910581.1"
                     /translation="MSLLLNREHTNGQVTNASYAKVIETVLKSGVQADDRTGTGTLST
                     CYVPSYYMLTGGTVPLISGKAVNLKPLLVELEWYLKGTGNIQFLKDNGVKIWDAWADE
                     NGDLGPVYGKQWRRWEDTRIVSHSEYLSKIATFRERGYKVEGYLGISEDRVVLSREID
                     QLQRIVDTLRTNPTDRRIMLNAWNVGELEDMKLPPCHFVFSLWSRELDFETRLTMATD
                     IGLQHSRLGYESIYTKMLYDLEMDGSVTEAELDELGIPKRILNSCLVQRSVDTFVGMP
                     FNIAGYGILTHFLAKITGHMAGAFVHFGFDVHLYNNHMEGVCELMKRQAPEHSDPVVI
                     FPHEWSELDDFKWDEVLILGYDPLSWIKVPVAV"
BASE COUNT         1758 a         1585 c         1340 g         1768 t
ORIGIN      
        1 aaacctgaac tgaacgaacg aagtgagtga agttcacctc gaacgaagtg agaggttgtc
       61 ttttcaggta atactctccc agggaggtga gtataaaaat ccctcaccaa cctggtcgtt
      121 tcataacctg aaaagttatg gtctaagtct actgccagct tagacttggg aagttatgga
      181 tgacagcacc ccagaaccga gatcttccca cactttatga aggggagtac tggattcaac
      241 ctctcgaaac accccagact cgacaatcat aaagtgaccc ttgtctccgc tcactttggt
      301 tcccccttcc ccgacaccta aacggcacca gttctacgct ggtagtgagc ttttttaaac
      361 ctgacgccag tgacgcttac ccccacccat caggtcgagt ctccagtcta cgactggaaa
      421 ctatcagatc ttagcactta caattcactt tatgaatagt ttgtacttat ctatcattgt
      481 gattgattat tttctctacc atgtagttga acattcaagg caaacacctc attgatcaac
      541 tcacctaaaa gctgttcaac aagttcgcga tgctcgccaa gatgcaagca cttaattgcc
      601 cattcgtaca agttgaaagc ctgctcacga tctttcatca tttcacgagc ctgggccaga
      661 aaatcgctct ctacgagcgc aacgacatta gtcgggtatg acatgttggt ttccttaaag
      721 tggttcataa aatcgatttt aaagcgtctg ggaaggggtt ctagtggatt ctgtagtgat
      781 gttcaggtct ggagtctctg atacaaaaca gcctgcttcc gtatattaat aattaatatg
      841 tagttattta tatatacaga agcaggctaa ttacagacgc cagaacaaac taagcagcct
      901 tctttgatgg tctttttttc ctgatcacat tcgcaggatc ataccctccc agacttttca
      961 tgacgtccaa agggatcttg cttatagagt gcccagcgtc ctgacagaat cctcgaaaaa
     1021 caacgagcat actgccacct gggttgatgt ttacttctac ctgacccaga ttgacgtcag
     1081 gctcgacaaa cgctacgcgg ccaccagaca gaataatcgt ctcattcgca cactctgttg
     1141 cacgctcata ccactttgta tctagggatt gaggaattag catcactgtt gtaactccgc
     1201 gagcctgctc acgtatagcc gcatcaatcc agggggagat tttggagtag ggtggattga
     1261 ggaaggcaat tgtacctggc tgtccccatt cagatttcaa agcatcacgc tcaacgccta
     1321 tgaaattagg cagctttgca ttgtccttgt tgcaggcaac gtcaacatca aatgtcactc
     1381 caagataacg ttgaatggca gaaaacagcc agtcaggtgt tctccagagg tcacgaagag
     1441 agttgtcgcg ctttcgctgt ttgatttttt cggctgcaat catcttcttt ctcgataggt
     1501 aagtaaatac ctatttttgc atttgatttt atgtctggca atcaaataaa acgcgccaga
     1561 aatgcaaacg agaagctctc tggcgcgttt ttgtggtgct gggtatgtga ttggcttacc
     1621 cagcactata aagctctcta tgggctttat tttcagtagg ggaacgtcat ccaatagtgt
     1681 actccgcgac aaacttctta acgatcggtg attcttcgtt caatgttaga tttccaccgt
     1741 ctctaacggc aattcctgtc gctggaaata cagccatcat ttgcccagcc tgagttgacg
     1801 cagtgttaag cggatacggt ttttctggat tgctcattaa agcaatctta atactattac
     1861 ttgtagcctt cttttctgtg agaaggtgtc tcatggaaat taccgtgtaa acactaatat
     1921 ctggacctcc gctaaaccaa ttaagaaggt ttaatatctt atcctttgct ttgacaggcg
     1981 cgttatcgta agcgtctaaa aaaatatctt tctctaaacc cgctttattg aaaaacgcat
     2041 catctttatc acttagagca aaacgaggac ctcttgcagt acgcgctttt ttatttgtgt
     2101 tgctatcatt tgactgttga acttttaatt catcattaat ctcaacacac ccttcaacct
     2161 cgatgatccc caactgttct ggttcgtttt cggcagcttc accactttca gcgttaatta
     2221 cgtttatagg ctcatgcgct aactccaact catttaaagc tgagctgaaa tcacctgctt
     2281 tttcttcatt gaacagttct tcggcaacat catcagaact catggctacg ccagcatcta
     2341 ggccgagcag cagatcgtca atatcatcag tattttcaac ctgtgcagct ttttttactg
     2401 gttcatcaag tgattgcaac atggctgcca gctcgtccag atcatcattt gtcattacat
     2461 tttcaaccga actcatgtaa gtctccttct gtttggttac atttgcgttt cgatggctac
     2521 attttgacta aacctatcag gcggaaaagt attaaataca ggcaggaaat gattgaaagg
     2581 cgactgttct ttaaaagaaa gcctacttta aactttttaa agtaggcttt tgttttacac
     2641 aatttcagag ggtaatttaa aaaatccgta cttattacgt gccttaaaga agcattgcat
     2701 cattaagttt gtgtcatata aggcactatg tgctttggca tgatcgtata taaagccaag
     2761 agagaaggcc aactcttcaa ggcggggacg tttaccgtct tctgtggccc acaatcccga
     2821 taacatagta tctataacag gaacttctgg cagtcttact ccataccctg aaaactcgtg
     2881 tcgaataaat ggtatatcaa atgcttcgcc gttatgagca acccacacac tacatgcccc
     2941 catataagcg ccaatttcgc tggcatgatt tgacaacaaa ggttcagctg cgagctgttc
     3001 caatgaaatg ccatgaacgg cttgagcttt cggatctatg tttctgcgag ggttaaaacg
     3061 catctccaga ctatcaatat gtctctgtgt ctccagttca tagcgggtaa ttgcgatttc
     3121 aataatttta tgaccagcaa ggaaatccag tcccgtagac tcgatatcga tgcctgcaac
     3181 aatcgttgtc atcagaactc cttacaactt cttagcgccc ttaagtagtg cgctacgtac
     3241 aaattgagcc gcttttttca aagtttcttc actggtttca cagacaatcg gatctttcca
     3301 cttacctgac acagtgttga gaatattgat ttggttgcta tccagacaaa cagagatata
     3361 aagcaccgtg ccaccagcca gcatcaaatg cataggaaac agaggctttt tactgccttc
     3421 actgaactga gacatggcga tgttaatggc ttcacccact tgtgtaccaa caagcccctg
     3481 cgcagtttcg aatatggcac gaatagccag acgagcctct cgatcgctca taagcgaccg
     3541 ggaatgttcg tccgctacac gaatcaaagc ctctgttgtt ttccgatcca gttcatcttc
     3601 aagcaacatt tcactaaaca ttttttatcc ttaacccatt ttgagagttt tattgtcgca
     3661 atgtcgaaga ggcgaacaat ttctaatgaa cggtcactcg gccacagaac cggttttcta
     3721 tcttttctac agcgttatct agcattctca tcactgatct ggctcttgct tcaaccgtgc
     3781 gatatcggtt ggaaacgacg aaggtttgca gatctcccct ttgcggatgc aggtgaattt
     3841 ttgtcagttc cccgcacatc aacgcatcaa tacgagcgac atacagacgg tctaatgatg
     3901 ctcgttgtga tcgcaagtaa tctcgctctg agagagcgac tcccgggcgt aagccagcaa
     3961 tggcgttaaa gtttgaaata gcagttttgt ggcaaaactt ttcgatatcg agggctaact
     4021 caatacaccg atcttcattt gtatggccga ccaaatccaa cgtgtaggcc ataacatcag
     4081 caggtgtgcg atcgataaca aagccttcaa caccacgcgt aagtagctct atatgctttg
     4141 ctatttccat ttgaatctga agccgctcat atagaggcat agaatcacct actcgaacac
     4201 cgaggcgact catcaaactt ccgacaccag catccacgta agggattccg taatgtttgt
     4261 cgatatattt agccagggtt gttttcccac tgccctgagc accagtgatc ccaatccggt
     4321 aatccattac gaccttctgt atacgatctg cgaaaagcca ggctcttcct cgctggctcg
     4381 ttgggtataa gctgtttcaa caggcacaaa gcctaaatta cgcatcatgc ttgccgggaa
     4441 gaatgcatcg gcgtttggca catcaacgcc gatgtgggag agccaaatct cttcaacatg
     4501 cggcatgaat agcgagtaaa tttgttcccc gccaatcacc cacacaggct ctggtaatcg
     4561 caacacatca tccacaccag cagggtagaa tccatttggt acaaagccat gagagcgcgt
     4621 caaaacgagg ttatgacgct ctggaagagg gcgtttgaga ctttccatcg ttttacgccc
     4681 cataacgaca gtggcgtttt tggtgagtct cttaaatagc ttcagatcgg tagaacaacg
     4741 ccaggggagt tcattcgcta tgccgatctc atagttacgg ccaacagcag cgatcatctt
     4801 catttgctca cctcatagat gaccggtcga tggtgagagt cggctaaagc ggcacggagt
     4861 cgaggatcgt gcaccagcgc agcgataagc agatcgcctt tatgctccgc taatgtgcgc
     4921 ttaatatgct tctcataact ggtgccattc ggtacgagat gaagccagtc ataatcaacg
     4981 ccaaaatctt tcagccatcg tttagttggc gcttcaagag tttcagagcg gctactgata
     5041 agcaccactt cagcccctga gcgagcaaaa ccacgcaaca tacggctggt ggggaaaatg
     5101 agttcatcac cggcaatgag ctggcctgca tccgcgtcag atactgattt acgatggctg
     5161 gcatttgcca gcacaccttc aatttcgcat aagacataca tacctcttgc catatcacac
     5221 cgccactgga accttgatcc acgatagagg gtcatagcca agaattaaaa cctcgtccca
     5281 tttgaaatca tccaactctg accattcatg agggaaaata acgaccggat ctgaatgctc
     5341 tggagcctgt cgtttcatta gctcacacac accttccatg tggttgttgt acaaatgcac
     5401 gtcaaagcca aaatgcacaa atgcaccggc catgtgaccc gtaatcttcg cgagaaaatg
     5461 agtgagaatg ccatagccag caatattgaa tggcatacca acaaaagtgt ctacgctacg
     5521 ctgtacgagg caggagttga ggatgcgttt ggggattcca agttcatcca gttcagcttc
     5581 agtaacactg ccgtccatct ccagatcgta tagcatcttg gtgtagatag actcgtaacc
     5641 gaggcgactg tgttgaagac caatgtcagt tgccatcgtt aaacgggttt caaaatccag
     5701 ctcacgactc cacaaagaga agacaaagtg gcaaggtggc agtttcatat cctcaagctc
     5761 gcctacgttc catgcgttaa gcatgatgcg acgatcggta gggttcgtgc gcagtgtatc
     5821 gacaatacgc tgtagctgat cgatttcacg ggacagcact acgcgatctt cactgatacc
     5881 caggtatccc tcgactttgt acccgcgttc acggaaagta gcgatcttgc tcagatattc
     5941 actatggctc acgatgcggg tatcttccca tcgacgccac tgcttaccgt aaaccggccc
     6001 caaatcgcca ttctcatcgg cccatgcatc ccaaatctta acgccgttat ccttgagaaa
     6061 ttggatgttg cccgtgcctt tcagatacca ctcaagttcg acaagcagtg gcttaagatt
     6121 taccgccttt ccagaaataa gcggcacagt cccaccagta agcatgtagt aagagggaac
     6181 gtagcaggtg cttaaagtac cagtgcctgt gcgatcatca gcctgcacgc cgcttttaag
     6241 caccgtctca ataacttttg catacgatgc gtttgttacc tgaccgttcg tatgctcacg
     6301 attcaaaagg agtgacacaa aaacctcaca aagataagta accacttatt cattacgtga
     6361 gtttacattt attgagagaa caaggccaga tagataaaaa aatggtggcc ataggccacc
     6421 aaaaacgaat agttgtttca ggaatttaac g
//
LOCUS       DBANUU010000206         6434 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000206 DBANUU010000000
VERSION     DBANUU010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.209"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(7..273)
                     /locus_tag="ACIE68_004375"
     CDS             complement(7..273)
                     /locus_tag="ACIE68_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910582.1"
                     /translation="MTLTDKQKDIIKTLNLGYERGHLLDLDELLEVLPYKTTKQSIQF
                     SIRALIKKGLVEKGHTRQRSDNRYHRRTLGLTTLGRAKAKLLVM"
     gene            complement(283..1173)
                     /locus_tag="ACIE68_004376"
     CDS             complement(283..1173)
                     /locus_tag="ACIE68_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910583.1"
                     /translation="MTDIIIKRYRPEEFPRHLDFLERMTVTKGTVEDWHALKSLHYKT
                     DGKPFAPTYYRCELDDRLVGVVVMAYPKLLLAPRHRMFPKLKPTTNTTVANQYWGRYV
                     NNNFAVISRSVVDTQYRGVGVSYRMINLVSRMHDRPIIEIQSSMSKYNPFAMKAGFKF
                     IRPERPKSYESALRVFQRHFRSDPGDNEAIVKELFAMSESRRRRALRDLVADYHKNSS
                     LAKAGRNRGTTIQDIADSLVDEASIVKLLKDIHNLSFTSPLYGVYRNPDFGRRLPDTL
                     PLLAFDKQPLDKPLEIALPA"
     gene            complement(1170..1835)
                     /locus_tag="ACIE68_004377"
     CDS             complement(1170..1835)
                     /locus_tag="ACIE68_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFX5910584.1"
                     /translation="MSKYIINVSFQTRVNKTTRTLEIAESFGLGLDEKEWTLYDNLEL
                     EVKQGDVVYITGQSGSGKSVVLRELQRQMKDEGLSVASIDDFTFDNEVNVIDQLGKTT
                     SDALGLLSMAGLNDAYLFVRKPSEMSDGQKYRLKIAKLIESGAKVWAADEFGAVLDRV
                     TAQVVASNLQRAARKVGATVMVATTHEDLKNALRPDMQITKHYKERVKVEYHNGSHDE
                     VHL"
     gene            complement(1832..2500)
                     /locus_tag="ACIE68_004378"
     CDS             complement(1832..2500)
                     /locus_tag="ACIE68_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HFX5910585.1"
                     /translation="MTKKFEIVYRDPADLIPYEMNAKKHDEQQIRDLAAAIKKRGFDQ
                     PITVDKNDVIITGHGRREAAIFAGLERVPVIVRDDLSDDEVRAKRLEDNRLASIDYDA
                     IKLQKELESLVLDDIEVFGFEERELNVLVGSMTEEMDTDSLVIDLGEETKRQKDEHTE
                     ISREVAAEEVRVVDVLGFKTLPAGSAIVVGDLLAHMEEMTGESGVDAFVAYAEKISSG
                     EMAA"
     gene            complement(2500..3180)
                     /locus_tag="ACIE68_004379"
     CDS             complement(2500..3180)
                     /locus_tag="ACIE68_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFX5910586.1"
                     /translation="MDVKITILQVDVANLRPNTWNTNSVGAQNFEKLKGSIEKLGFFK
                     PILARELEDGFFEILGGEHRWRAAIEQGISTVPVLSVGKISDVVAKQMSLVDNERYGE
                     DDQIALQRFIEEIQSELDYQLSEIAPYDDEISMVLAKEAAIDLEALEALSRGSDEPVD
                     TDKREKTERVGAEHQTMRFKVTFDASDRVAETIKNIIKEQAINTGNEMENAGEARVWL
                     VDYYKERI"
     gene            3263..4822
                     /locus_tag="ACIE68_004380"
     CDS             3263..4822
                     /locus_tag="ACIE68_004380"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFX5910587.1"
                     /translation="MTVRIVSNAVNALISGADDKVKQLVQQMLSYEVETGDWKGTSTM
                     FNWSKNSFPAGFAKPVAANLNKAGIKCVHIRKDKAPALGKPNPAVNPFPYNPDYAYQD
                     QTVETLVREGMMIAQIATGGGKSNVACKAAARIGRMTLFLTTRSVLMFQMAENFQRSI
                     DYRAKNGEPWLKDQKVGVIGSGEFQVSRHINVATVQTLASFLEEPPRDATPDKKSYHL
                     KRRELVKRFLSSVSLLILEEAHESSGSNFYDIARLCVNADYRLALTATPFMKDSTEAN
                     MRLMAVAGRIEIKVTEKYLIDRGILAKPYFLYHKVAYKPDEARIKAELANKHLNFRVG
                     MSTAYQKAYQLGIVYNLGRNEAIVREALLYKQHSLNCMTLVRLKRHGQILMEMMKESG
                     LRVDFIYGESNQATRQAKLNSLASGEIDVLIGSTILDVGVDVPSVGAVILAGGGKAEV
                     EMRQRVGRGLRAKKNQANVCFITDFIDISNKYLLSHSYERKHIIDTTPGFAEGVLPID
                     GAFDFGVLKRD"
     gene            4825..5103
                     /locus_tag="ACIE68_004381"
     CDS             4825..5103
                     /locus_tag="ACIE68_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910588.1"
                     /translation="MSEKKTTYCQVALSDKANDKLGKFQVKLKEKNIKMSKAEVINTI
                     LEQLTMADFDKVISSVGASAKTREKIMRIYENSNMTKEDLETLLSRLK"
     gene            5130..5594
                     /locus_tag="ACIE68_004382"
     CDS             5130..5594
                     /locus_tag="ACIE68_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910589.1"
                     /translation="MKSPILFTFCSKTIMKYSLLPLLTLPVLMLTACQSRPISIHDAK
                     PAPQAKVFKYQSAAPATLVVMRDTGFVGAGCDASIFINGETVAKLATGEKATFHLNAG
                     ELIVGASLEGAGLCALNPARQERETTLKNGDTKAFRVFTSNSGDIDILPTTL"
     gene            5599..6123
                     /locus_tag="ACIE68_004383"
     CDS             5599..6123
                     /locus_tag="ACIE68_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFX5910590.1"
                     /translation="MTTKDITYGIQAELWPRDYTNVEKLLMFWRREQIPVRVTLEDGQ
                     AFCMYVYGLMPSRNKVDLCPAPFDKENRIRLPLERISTIESGVVDSIAHDFKGRLTVH
                     PDYVDNRPSRRDFFAICNQAYKANKSIRVYMADGREIEGVSAGADACQVTLRVENGRK
                     IVVLFDWVERILPF"
     gene            6139..>6434
                     /locus_tag="ACIE68_004384"
     CDS             6139..>6434
                     /locus_tag="ACIE68_004384"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910591.1"
                     /translation="MLPPLFLLCSVAANAMDYKPVIQSLMNDVCSTSQNVSVCMYQFS
                     AAVKAGKAIGENVELCKKVANEERAMLDCESSESSAQFVDALFDTNRKAVESVQ"
BASE COUNT         1721 a         1540 c         1485 g         1688 t
ORIGIN      
        1 gaccgattac atcaccagta acttcgcttt ggctcgacct aaagtggtta acccaagagt
       61 tcggcggtga tagcgattgt cactgcgttg gcgcgtatgc cctttctcca ccagcccctt
      121 ttttatcaga gcgcgaattg agaactggat actttgcttg gttgtcttgt acggcaaaac
      181 ttcaagcaat tcgtccaggt caagtagatg acctcgctca taaccgagat tgagcgtttt
      241 gatgatgtcc ttttgtttat cggttaacgt catggcaaat ccttatgccg gtaaagcaat
      301 ttctaacggt ttatccaaag gttgtttgtc gaatgccagc agtggcagcg tgtcaggcag
      361 tcgacggcca aagtcagggt ttcggtacac accatacaac ggagacgtaa agctcaggtt
      421 gtgaatgtcc ttgagcagct tcacaatgct ggcctcgtcc accagactgt cggcaatgtc
      481 ctgaatcgtc gtgccacgat tccgcccagc ttttgccagg gaactgttct tgtggtagtc
      541 cgccaccaga tcacgcagtg cacggcgacg acgagactcg ctcattgcga acaactcttt
      601 gacgatcgcc tcgttatcac ccgggtcgga acggaaatgg cgctggaata cacgcagtgc
      661 actttcatag ctcttcggtc gctcagggcg gatgaactta aaccctgctt tcatggcgaa
      721 gggattgtat ttgctcatcg aggactggat ctcaatgatt ggccggtcat gcatcctgct
      781 aaccaggtta atcattcgat aagagacgcc tacgccacga tactgagtat ccacaactga
      841 gcgactgatc accgcaaagt tgttattcac gtaccgaccc cagtactggt tagccacggt
      901 ggtattagtg gttggtttca acttaggaaa catgcggtgg cgaggtgcca gcagtagttt
      961 cgggtaagcc ataaccacga cgcccaccag acggtcatca agttcgcagc gatagtaagt
     1021 tggcgcgaaa ggtttgccgt ctgttttgta gtgaagcgac ttaagagcgt gccagtcctc
     1081 tacagttccc tttgtgacag tcattcgctc cagaaagtcc agatgacgcg gaaactcttc
     1141 agggcggtag cgtttaatga tgatgtccgt cataaatgaa cctcatcatg actaccattg
     1201 tgatattcca ccttcacgcg ttctttgtag tgcttggtga tctgcatatc cgggcgcagc
     1261 gcgttcttca ggtcttcgtg agtcgtcgcc accattaccg tcgcaccaac ctttcgagcg
     1321 gcacgctgga ggttagatgc cacaacctga gcggttacac ggtctagaac agcaccgaac
     1381 tcgtcagcag cccacacttt agcgcctgac tcaatcagtt tggcaatctt gagacgatat
     1441 ttctgaccgt ctgacatttc agaaggcttg cgaacaaaca gatatgcatc gttcagacca
     1501 gccatagata acaacccaag cgcatcgctg gtcgttttgc ccaactgatc gatgacgtta
     1561 acctcattat cgaaggtaaa atcatcgatg gaggctacag acagcccttc atccttcatc
     1621 tgtcgttgca actcgcgcag cacaacggat ttgccggaac cggattggcc ggtgatgtac
     1681 accacatcgc cctgcttcac ttccagctcc agattgtcgt aaagcgtcca ctctttttcg
     1741 tccaggccaa gcccgaacga ctcagcgatt tccaacgtgc gcgtggtttt atttacgcgt
     1801 gtctgaaacg atacgttgat gatgtatttg ctcatgcagc catctccccg gaagagatct
     1861 tctccgcata tgccacaaat gcgtctaccc cgctttctcc cgtcatttct tccatgtggg
     1921 caagcaaatc accaacaaca atggcagagc cagcaggaag cgttttaaag cccaatacgt
     1981 cgacaacacg tacttcttcc gctgcaactt cacgactgat ctcggtgtgt tcatccttct
     2041 gtcgtttagt ttcttcgcca agatcgataa ctagcgagtc ggtgtccatt tcttctgtca
     2101 tgctgccaac gagaacattc aactcacgct cttcaaagcc gaaaacctcg atatcgtcca
     2161 gaacaagcga ctcaagctct ttctgtagct taattgcatc gtaatcaatg ctggcaagtc
     2221 ggttatcttc aaggcgcttc gcacgaacct cgtcatcact gagatcatcg cgaacaataa
     2281 ccggtacgcg ctcaagtcca gcaaaaattg cagcctcacg gcggccgtgg ccagtaataa
     2341 ttacgtcgtt cttatcgacc gtaattggct ggtcaaatcc gcgcttttta atggctgcgg
     2401 ctagatctcg gatctgctgt tcatcatgct ttttggcatt catctcatag ggaataagat
     2461 ctgccgggtc gcgatatacg atttcaaact ttttggtcat tatatacgct ccttgtagta
     2521 gtcgaccagc cacacccgag cctcaccagc gttctccatt tcattaccgg tgttaatagc
     2581 ctgctctttg atgatgtttt ttatggtttc tgcaacacga tctgacgcat cgaaagttac
     2641 tttgaagcgc atggtctgat gttccgcccc cacacgttcg gttttctctc gtttgtcggt
     2701 atcgacaggc tcatcactac cacgagacaa cgcctccagt gcttcaaggt cgattgccgc
     2761 ctcttttgct aaaaccatcg agatttcgtc gtcatacggg gcgatttcag acagttgata
     2821 gtcaagttct gactgaattt cttcaatgaa gcgttgcaat gcgatttggt cgtcttcacc
     2881 gtatcgctcg ttgtccacca gtgacatctg tttagctacg acatcgctaa ttttgcccac
     2941 agaaagcact ggaaccgttg aaattccttg ctcaatagcg gcacgccagc gatgttcgcc
     3001 gccgaggatt tcaaaaaatc catcttcaag ttcacgagcc aaaattggct taaaaaagcc
     3061 caatttttcg atagaacctt tcagtttttc aaaattctgc gcaccaaccg aattggtgtt
     3121 ccaggtattc gggcgaaggt tggcaacatc aacctgcaaa atcgtgattt ttacatccat
     3181 tttattgcta caatccacta agtaatcact tacttattat aatagccaaa taacatacaa
     3241 aaggcactaa ggaaagaggt ttatgactgt tcggattgta tctaacgcag ttaatgcgct
     3301 tatttctggc gcagatgaca aggtaaagca actggtgcaa cagatgttga gctacgaagt
     3361 cgagactggc gactggaagg gcacaagcac aatgttcaac tggagtaaaa actcgttccc
     3421 tgcaggcttt gccaagcctg tagcggcgaa cttgaacaag gcgggcatca aatgtgttca
     3481 tatccgcaaa gacaaagccc cggcgcttgg aaaaccaaat ccggcggtta acccattccc
     3541 atacaatcct gattatgcgt atcaggatca gactgtggaa acactggttc gagagggaat
     3601 gatgattgcg cagatcgcta ctggtggcgg gaaatctaat gttgcctgca aagcagctgc
     3661 acgtatcggt cgaatgacat tatttttaac aacccgctct gttctgatgt ttcaaatggc
     3721 cgaaaacttc cagagatcca tcgactaccg cgccaaaaat ggcgaaccgt ggttaaaaga
     3781 ccaaaaggtt ggagtcattg gctcgggtga gttccaggta tcacgacata tcaacgtcgc
     3841 tacagttcaa actcttgcaa gtttcctcga agaaccacca cgcgatgcaa caccagataa
     3901 gaaaagctac cacctcaaac gtcgggagtt ggtgaaacgc ttcctttcaa gtgtctctct
     3961 tcttattctg gaagaggcgc atgagtcttc aggctcaaat ttctatgaca tcgccagatt
     4021 atgtgtgaac gcagactatc gtctggcgct tacagccacg ccgttcatga aggattcgac
     4081 ggaagccaac atgcgcctga tggcggtggc cgggcgaatt gaaattaaag tcacagaaaa
     4141 gtacctgatt gatcgaggca ttctggcaaa gccgtacttc ctttatcata aagttgccta
     4201 caagccagac gaggccagaa tcaaggccga acttgccaac aaacacctca attttagagt
     4261 tggtatgagc accgcctacc aaaaggctta tcagttgggg atcgtgtata atttgggacg
     4321 taacgaggcc attgttcgcg aagcattgct ctataagcaa cattctctca attgtatgac
     4381 tctggttcgt cttaaacgcc acgggcaaat cctgatggaa atgatgaaag agtccggcct
     4441 tagagttgac ttcatctatg gggaatctaa ccaggcgaca aggcaagcaa agctgaacag
     4501 tttagcgtct ggagaaatag atgttttaat aggctcgact attctggatg tcggtgttga
     4561 tgtgccaagc gttggtgcgg tcattcttgc tggtggtggg aaagcagaag ttgaaatgag
     4621 gcagcgtgtc ggtcgtggct tacgagccaa aaaaaatcag gcaaacgtgt gttttatcac
     4681 tgatttcatt gacattagca acaaatacct gttgtctcac tcttatgagc gaaaacacat
     4741 catcgacacc acacctggct ttgcagaagg tgtattgcct attgatggag cattcgattt
     4801 tggagttctg aaacgagatt agttatgagc gaaaagaaaa caacttattg tcaggtagca
     4861 ttgtctgata aggccaatga caaacttgga aagtttcaag tgaaactaaa agaaaaaaat
     4921 atcaaaatgt ctaaggctga agtcataaat accattctgg aacaattgac aatggccgac
     4981 tttgacaagg ttatatcttc tgtcggggct tccgctaaga ctcgtgagaa aatcatgcgt
     5041 atctatgaga actctaatat gacaaaggaa gatctcgaaa cgctattaag cagattaaaa
     5101 taatcacgct atcaattagg gagacaaaga tgaagtctcc catactgttc actttctgtt
     5161 ctaaaactat catgaaatac tcgcttctcc ctctactaac gttaccagtg ttgatgctta
     5221 ccgcgtgcca atcacgccca atatcaatcc atgacgccaa accagcaccg caggccaaag
     5281 tgttcaagta tcaaagcgca gcgccagcta cgttggtggt aatgagagac acaggttttg
     5341 taggagctgg atgtgatgct tccattttca tcaacggcga aactgtcgca aaactggcta
     5401 caggcgaaaa agcgactttc catttaaatg ccggagaatt aattgtgggt gcatctcttg
     5461 aaggtgcagg tttatgtgct ctaaaccccg ctcgtcaaga gcgagaaacg actctgaaga
     5521 atggagacac aaaagcgttc agggtgttca ccagcaattc tggtgacatc gacattctgc
     5581 caacaactct gtgatgacat gacaaccaaa gatattacct acggtatcca agctgaactc
     5641 tggccgcgag attacactaa cgttgaaaaa ctactgatgt tctggcgcag agaacaaatt
     5701 cctgtaaggg tcactctcga agatggccag gcgttttgca tgtacgttta tggcctcatg
     5761 ccatctcgta acaaagttga cctttgccca gccccttttg acaaagaaaa tcgtataagg
     5821 ctcccacttg aacgcattag tacaattgaa tcaggtgtgg ttgacagtat cgctcacgat
     5881 ttcaaaggtc gactaacagt tcaccctgat tatgtcgata atcggccatc acgccgcgat
     5941 ttttttgcaa tttgcaacca agcctacaaa gcaaacaaat ctataagggt gtacatggcg
     6001 gatggccgtg aaattgaggg ggtgtcagca ggcgcagatg cttgtcaggt tacactacgt
     6061 gtcgagaacg gtagaaaaat agttgttttg ttcgattggg ttgaacgaat tttgcctttt
     6121 tgagttatga aatcgatatt gttaccacca ttatttttac tttgctcagt tgcggcaaat
     6181 gcaatggatt ataagcctgt cattcagtcg ctgatgaatg acgtgtgctc aacgtctcag
     6241 aatgtatcag tttgcatgta tcaattttcg gcagccgtaa aagcaggaaa agcgataggt
     6301 gagaatgtgg aactgtgtaa gaaagtggca aatgaagaac gggcaatgtt ggattgcgaa
     6361 tctagcgagt catcggcaca gttcgttgat gcgctatttg acaccaatcg taaggctgta
     6421 gagtctgttc aata
//
LOCUS       DBANUU010000207         6394 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000207 DBANUU010000000
VERSION     DBANUU010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.210"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            108..1037
                     /gene="pfkB"
                     /locus_tag="ACIE68_004385"
     CDS             108..1037
                     /gene="pfkB"
                     /locus_tag="ACIE68_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFX5910592.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGTATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1138..1428
                     /gene="ghoS"
                     /locus_tag="ACIE68_004386"
     CDS             1138..1428
                     /gene="ghoS"
                     /locus_tag="ACIE68_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFX5910593.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            1534..2394
                     /gene="yniA"
                     /locus_tag="ACIE68_004387"
     CDS             1534..2394
                     /gene="yniA"
                     /locus_tag="ACIE68_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFX5910594.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2435..2971)
                     /gene="yniB"
                     /locus_tag="ACIE68_004388"
     CDS             complement(2435..2971)
                     /gene="yniB"
                     /locus_tag="ACIE68_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFX5910595.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3118..3786
                     /gene="hxpB"
                     /locus_tag="ACIE68_004389"
     CDS             3118..3786
                     /gene="hxpB"
                     /locus_tag="ACIE68_004389"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFX5910596.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3949..4539
                     /gene="ydjM"
                     /locus_tag="ACIE68_004390"
     CDS             3949..4539
                     /gene="ydjM"
                     /locus_tag="ACIE68_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFX5910597.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4672..6063
                     /gene="tcyP"
                     /locus_tag="ACIE68_004391"
     CDS             4672..6063
                     /gene="tcyP"
                     /locus_tag="ACIE68_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFX5910598.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(6067..>6394)
                     /gene="ydjO"
                     /locus_tag="ACIE68_004392"
     CDS             complement(6067..>6394)
                     /gene="ydjO"
                     /locus_tag="ACIE68_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFX5910599.1"
                     /translation="KNESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDIMSIPDSS
                     LRKRLGYEVLSFSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQ
                     NGPLPA"
BASE COUNT         1588 a         1564 c         1588 g         1654 t
ORIGIN      
        1 atatttatct gcaaaatttt aaataaagct ccaataaatc atattgttaa tttcttcact
       61 ttccgctgat tcggtgccag actgaaatta gcctatagga ggaaatgatg gtacgtatct
      121 atacgttgac acttgcgccc tctctcgata gcgcaacaat taccccgcaa atttatcccg
      181 aaggaaaact gcgctgtacc gcaccggtgt tcgaacccgg gggcggcggc atcaacgtcg
      241 cccgcgccat tgcccatctt ggaggcactg ccacagcgat cttcccggcg ggtggcgcga
      301 ccggcgaaca tctggtttca ctgttggcgg atgaaaatgt ccccgtcgct actgtagaag
      361 ccaaagactg gacccggcag aatttacatg tacatgtgga agcaagcggt gagcagtatc
      421 gttttgttat gccaggcgcg gcattaaatg aagatgagtt tcgccagctt gaagagcaag
      481 ttctggaaat tgaatccggg gccatcctgg tcataagcgg aagcctgccg ccaggtgtga
      541 agctggaaaa attaacccaa ctgatttccg ctgcgcaaaa acaagggatc cgctgcatcg
      601 tcgacagttc tggcgaagcg ttaagtgcag cactggcgat tggtaacatc gagttggtta
      661 agcctaacca aaaagaactc agtgcgctgg tgaatcgcga actcacccag ccagacgatg
      721 tccgcaaagc cgcgcaggaa atcgttaata gcggcaaggc caaacgggtt gtcgtttccc
      781 tgggtccaca aggagcgctg ggtgttgata gtgaaaactg cattcaggtg gtgccaccac
      841 cggtgaaaag ccagagtacc gttggcgctg gtgacagcat ggtcggcgcg atgacactga
      901 aactggcaga aaatgcctct cttgaagaga tggttcgttt tggcgtagct gcggggagtg
      961 cagccacact caatcaggga acacgtctgt gctcccatga cgatacgcaa aaaatttacg
     1021 cttacctttc ccgctaacaa aaacattccc ccagcattgg gggaatcatc accaacctgt
     1081 cggcaacgcg tttctccgac tatgctcaaa agtcatgtga taacaaaggg gtgaactatg
     1141 gccagtggcg atcttgtccg ttatgtcatt accgtcatgt tgcatgagga tacattgact
     1201 gaaattaacg agttgaataa ttacctgact cgcgacggtt ttttgctcac catgacggat
     1261 gatgagggaa atatccatga gctggggact aacacttttg gacttatcag tacccaaagt
     1321 gaagaagaaa ttagagaact ggtttcgggg cttacccaaa gtgcaaccgg caaagatcct
     1381 gaaatcacca tcacgacctg ggaggaatgg aatagcaaca gaaaataaat ggtttttggg
     1441 caataatcag tctgtggtgt gcgttagctc gtgtttttac accgcattct tgcgctaacc
     1501 ttatgatctg gcagacaaca tgggagagac atcatgtggc aggcaatcag tcgtcttttg
     1561 agcgagcagt taggtgaagg cgaaatcgaa ctgcgtaatg aactgcctgg cggagaagtc
     1621 catgccgcat ggcatttgcg ctatgcagga catgactttt tcgtcaaatg tgatgaaagg
     1681 gaactgcttc ccggttttac cgccgaagcc gaccaactgg agttactgtc gcgtagtaaa
     1741 accgtcaccg tgcctaaggt ttgggcagta ggcgctgacc gtgactacag ttttctggtg
     1801 atggattatc tcccacctcg tccgctggat gcgcatagcg catttattct tggtcagcaa
     1861 atagcgcgtt tacatcaatg gagcgaccaa ccacaatttg gcctcgattt cgataacgca
     1921 ctctccacaa ctccacagcc caacacctgg caacgtcgct ggtcaacgtt ttttgctgaa
     1981 caacggattg gctggcagct ggaactggca gcagaaaaag ggatcgcttt cggcaatatc
     2041 gacgccatcg tagagcatat tcagcagcgt ctcgcctcac atcaaccgca gccttctctg
     2101 ttgcacggcg atttatggtc cggcaactgt gcgctgggtc cggatggccc gtacattttc
     2161 gacccggcct gctactgggg tgaccgagag tgcgacctgg cgatgttacc gctgcataca
     2221 gaacagccgc cacaaatcta tgacggctat cagtcagtat ccccgctacc tgccgatttc
     2281 cttgaacgtc aaccggttta ccaactctac acgctgttaa atcgtgcaag gttatttggc
     2341 ggtcagcatt tggttattgc tcagcagtca ctggatagat tattagcagc atgatatggg
     2401 ttgaagataa tggccgctcc gtgcggcctt ttgattaaat aaacccaagc agagaaaaga
     2461 atacataacc agcagcaata caaataaccg gcaggatata cagtgagaaa aactgcagga
     2521 aaatagtatg gtgcggaaca acaatacgtg attcaatttg ttcacgcgtt agcccctctt
     2581 cccctttggc tttttccaga atgagttgat cttcaacacc ttctcgcaag aagcgcgcct
     2641 ggcgactcat ccgggcaccg gaatcctgca acgccagtcc gacaaaaatc aggatgaaaa
     2701 tcacccagaa cataacgttc acgccaccat taaaattggg cgtcggggag ttataccaga
     2761 acagattcaa aaaaggcgta ttcgcctgca tcatatcgat catgacatga gtaaagtcga
     2821 gcatgaccgc attaatgcct tcctggtttt cctgccgggt attcataaat ttcagcaaag
     2881 aaatcaatgt agagatcaat gcaggtataa aaatcaccca acccaaaatc cttttcaaaa
     2941 cagcaatgcg tccagcttgt tgatacgtca tgagttctcc ttgattaaga cgcgtcgttt
     3001 cacttagttt acctgtagat atctgttttc gcccattctt taaaggcgat atgataggcg
     3061 cttaatcata agcacggctt aataccttac acataatgct ctaaaggaga ggttgtaatg
     3121 tcaaccccgc gtcagattct tgctgcaatt tttgatatgg atggattact tatcgactca
     3181 gaacctttat gggatcgagc cgaactggat gtgatggcaa gcctgggggt ggatatctcc
     3241 cgtcgtaacg agctgccgga caccttaggt ttacgcatcg atatggtggt cgatctttgg
     3301 tacgcccggc aaccgtggaa tgggccaagc cgtcaggaag tagtagaacg ggttattgcc
     3361 cgtgccattt cactggttga agagacacgt ccattattac caggcgtgcg cgaagccgtt
     3421 gcgttatgca aagaacaagg tttattggtg ggactggcct ccgcgtcacc actacatatg
     3481 ctggaaaaag tgttgaccat gtttgactta cgcgacagtt tcgatgccct cgcctcggct
     3541 gaaaaactgc cttacagcaa gccgcatccg caagtatatc tcgactgcgc agcaaaactg
     3601 ggcgttgacc cactgacctg cgtagcgctg gaagattcag taaatggcat gatcgcctct
     3661 aaagcagccc gcatgcgttc cattgtcgtt cctgcgccag aagcgcaaaa tgatccacgt
     3721 tttgtattag caaacgtcaa actctcatcg ctgacagaac tcaccgcaaa agaccttctc
     3781 ggttaatgac caggggcagt gatctcgctg cccctggttc tttatctgaa tttgcattca
     3841 actgacggat taatcgtcaa tttaagagaa agagttacac cgtcaccact tccgtgcact
     3901 gtataaaaat cctatactgt acgtatcgac agtttagtga gttttatcat gacggcggaa
     3961 ggtcaccttc tcttttctat tgcttgtgcg gtatttgcca aaaatgccga gctgacgccc
     4021 gtgctggcac agggtgactg gtggcatatt gtcccttccg caatcctgac gtgtttgtta
     4081 ccggacatcg atcacccaaa gtcgtttctt gggcagcgat taaagtggat atcaaaaccg
     4141 atcgcccgcg cttttgggca tcgtggtttt acccacagtc tgctggcggt atttgcgctg
     4201 ctggcaacct tttaccttaa ggttcctgaa agctggttca ttccggctga tgcgctacaa
     4261 ggaatggtgc tgggttattt aagccacata cttgccgata tgctgacacc cgccggtgtt
     4321 cccctgctct ggccatgtcg ctggcgtttc cgcttgccta tcctggttcc ccaaaagggc
     4381 aaccaactgg aacgttttat ctgcatggca ttatttgtct ggtcggtatg gatgccccat
     4441 tcattacccg aaaacagcgc ggttcgttgg tcatcgcaaa tgatcaatac cttgcagatc
     4501 cagtttcatc ggcttattaa gcatcaggtt gaatactaaa aaggcaaaaa tcacctttct
     4561 ggaataagca attccatttg aatataagag ccagctcaca gttctgttaa tcttgcgcca
     4621 acactatgac tgctacgcag tgatagaaat aataagatca ggagaacggg gatgaacttt
     4681 ccattaattg cgaacatcgt ggtgttcgtt gtactgctgt ttgcgctggc tcagacccgc
     4741 cacaaacagt ggagtctggc gaaaaaagtg ctggtgggtc tggtgatggg tgtggttttt
     4801 ggccttgccc tgcataccat ttatggttct gacagccagg tacttaaaga ttctgtacag
     4861 tggtttaaca tcgttggtaa cggctatgtt caactgctgc aaatgatcgt tatgccgtta
     4921 gtcttcgcct ctattctgag cgcggttgcc cgtctgcata acgcatctca gttaggcaaa
     4981 atcagttttc tgaccatcgg tacgcttttg tttaccacac tgattgcggc gctggtcggt
     5041 gtgctggtca ccaacctgtt tggtttgacg gctgaaggtc tggttcaggg tggtgcagaa
     5101 actgcacgtc tgaacgccat cgaaagtaac tatgttggta aagtctctga cctgagcgtt
     5161 ccgcagctgg tcttgtcctt tatcccgaaa aacccgtttg ccgatctgac cggagccaat
     5221 ccgacgtcaa ttatcagcgt ggtaattttt gccgcattcc tcggtgtagc tgcgctgaaa
     5281 ctgctgaagg atgatgcgcc gaaaggtgaa cgcgtcttaa ccgctatcga taccctgcaa
     5341 agctgggtga tgaaactggt tcgcctggtc atgcagttga ccccttacgg cgttctggca
     5401 ctaatgacca aagtggttgc aggttctaac ctgcaagaca tcatcaaact gggaagtttc
     5461 gttgtcgcgt cctacctcgg tctgctgatt atgtttgcag tgcatggcat tctgctgggc
     5521 attaatggcg tgagtccgct gaagtacttc cgtaaggtat ggcctgtgct gacgtttgcc
     5581 tttaccagcc gttccagtgc tgcgtctatc ccactgaatg tggaagcaca aacgcgtcgt
     5641 ctgggcgttc ctgaatccat cgccagtttc gccgcctctt tcggtgcgac cattggtcag
     5701 aacggctgcg ccggtttgta tccggcaatg ctggcggtga tggttgcgcc tacggttggc
     5761 attaacccgc tggacccgat gtggattgcg acgctggtcg gtattgttac cgttagttcc
     5821 gcaggcgttg ccggtgtcgg tggtggtgca actttcgccg cactgattgt gctgcctgcg
     5881 atgggcctgc cagtaaccct ggtggcgctg ttaatctccg ttgaaccgct tatcgacatg
     5941 ggccgtacgg cgctaaacgt tagtggctcg atgacagctg gcacgctgac cagccagtgg
     6001 ctgaagcaaa ccgataaagc cattctggat agcgaagacg acgccgaact ggcacaccgt
     6061 taattcttat gctggcaacg gtccgttttg tataggggcc gttgctttac tttttaaatg
     6121 ttccatcgcg gcgatgcata ctgactcata tttaaacaga tcgtcagcga aaaaaatgtc
     6181 caacttatca atacattcct ggctcaatga atgtgcctgt agtgagaagc ttaaaacctc
     6241 atacccgaga cgttttctaa gtgaactatc tggaatactc ataatatctt caagcacacc
     6301 acttcccatt cccggaagat ttagtggatt aaaaagaaga ttgatattaa gcattgatat
     6361 tatcgaagga aactttagga gactctcatt tttt
//
LOCUS       DBANUU010000208         6248 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000208 DBANUU010000000
VERSION     DBANUU010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.211"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(142..1812)
                     /gene="betA"
                     /locus_tag="ACIE68_004393"
     CDS             complement(142..1812)
                     /gene="betA"
                     /locus_tag="ACIE68_004393"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFX5910600.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDMGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(1826..3298)
                     /gene="betB"
                     /locus_tag="ACIE68_004394"
     CDS             complement(1826..3298)
                     /gene="betB"
                     /locus_tag="ACIE68_004394"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFX5910601.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSALQGQKIWASMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3312..3899)
                     /gene="betI"
                     /locus_tag="ACIE68_004395"
     CDS             complement(3312..3899)
                     /gene="betI"
                     /locus_tag="ACIE68_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFX5910602.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4028..6061
                     /gene="betT"
                     /locus_tag="ACIE68_004396"
     CDS             4028..6061
                     /gene="betT"
                     /locus_tag="ACIE68_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFX5910603.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT         1276 a         1723 c         1731 g         1518 t
ORIGIN      
        1 aaatatcagc aaaaagcccg catcatgaat actggatatg aagcatgaga gttacctcag
       61 tgtttatata aggattcggt ccccctctct ggaacggtaa ctctcaatct gatcggttcc
      121 tgcgttagtt cacatcacga ctcatttttt cgctctcacc ggcatcccat ttgccacaaa
      181 atatcccgcc gtgctcctcg gcagcgcttc ctgtccacga atcatatccg ctattttctc
      241 gccaatcata attgtcgtgg cgttcaaatt cccggtgata atctgcggca taatcgacgc
      301 atccaccaca cgcaggcctt ctaacccgtg tacgcggcct tcgccgtcaa ccacggacat
      361 ctcgtcgtaa cccattttgc aggtaccgca cggatggaag gcggtttcgg cgtggttacg
      421 cacgaactca tcgagctgtt catccgtctg gcattcgaca ccggggctga tttcgcggcc
      481 acgatactga tccagcgcgg gttgatgcat gatctcgcgg gtgatgcgaa ttgcgtcgcg
      541 gaactcctgc cagtcctgct cgtgcgacat gtagttaaac agaatcgccg gatgctggtg
      601 cgggtcgcgg gatttaatcc gcacatgccc acggcttggc gagcgcattg agccgacgtg
      661 gcactggaaa ccgtgctctt tcactgcatt cgagccgtta tagttaatcg ctactggcag
      721 gaaatggtac tgaatattcg gccacgcaaa ttcctcacgg ctgcgaataa atccacctgc
      781 ttcaaagtgg ttgctggcac caacgccagt gccgccaaac agccactccg caccgatttt
      841 cggctggttc caccactgca gggcagggta gagggaaacc ggttctttgc actcatattg
      901 cagatacatc tccagatgat cctgaagatt ttcgccgacg ccgggtaatt catgcaccag
      961 cggaatatca aactccgcca gcagttcagc gttgccgacg ccggagcgtt gcaggatctg
     1021 cggtgaggca atcgcgcctg cacataacag cacttctttg ttggccgttg cgcgggttgg
     1081 gatggtgctg tcgccttcca gccattcgac gcccaccgcg cgtttgccgt caaaaatgat
     1141 gtgatcggtc atagcgtgag tacgaatggt caggttagga cgcgatttgg cctgatcgag
     1201 atagccacgc gcggtgctgg cgcgacggcc ctgcggcgtg acggtgcgat ccatcggacc
     1261 aaaaccttcc tgctgataac cgttgagatc gtccgtgcgc gggtagcccg cctgcacgcc
     1321 cgcttcaatc atcgcttcaa acagcggatt gacgccgggt ttggaggtag tgacgctcac
     1381 cgggccatca ccgccgtgat agtcgttttc acccatatcg cgagtctcgg ccttgcggta
     1441 gtagggcagg cagtcgaggt agctccagtt ctccagaccg ggttcttgcg cccagttatc
     1501 gagatccagc gcattgccac ggatgtagca catgccgttg atcagcgacg atccacccag
     1561 acctttaccg cgtccgcact ccatgcggcg gttattcata aacggttcag gttccgtttc
     1621 ataggcccag ttgtagcgtt taccctgtag cgggaatgcc agggcagcgg gcatctgggt
     1681 gcggaagtca aagcgatagt ccgggccgcc cgcttcaagc agcagcacgg aggtattcgg
     1741 atcttcagtc agacgggtag cgagaacgtt gccggctgag ccggcaccaa taatgatgta
     1801 gtcaaattgc aaataaacct cctggttaga atatggactg gaatttagcc atctcaacct
     1861 ggatggactt cacctgggtg taactctgga gcgtcatcac gccgttctcg cgaccaatgc
     1921 cggagtgttt gtagccgcca acgggcatct ctgccgggga ttcgccccag gtgttgatcc
     1981 agcaaatacc cgcttccagc tgatgaatga cgcgatgcgc gcggttcagg tccgctgtca
     2041 cgatgcccgc cgccaggccg tagtcggtat cgttagcgcg gcgaatgact tcgtcttccg
     2101 actcgtaggt cagaatggac atcactggcc cgaagatctc ttcacgcacg atggtcatat
     2161 cgtcgctgca atcggtgaac actgtcggtg caacccatgc gccgttatcg aagccatcgc
     2221 ctttcagtac atcgccgccg cacagtacgc gcgcgccttc ctctttgcct ttggcgatat
     2281 agcgcagcac gttatcgcga tgcgggaagc tgaccagcgg gccgaagtta gtttgcggat
     2341 cgaaaacgtc gcccgcgcga atgcgctcaa cgcgcgccag aattttctgc tcaaatgcgg
     2401 ctttgcattt cgccggaacg aagacgcggg tgccattggt acacacctga ccggagctga
     2461 agaagtttgc catcatggcg atatcggcgg cgagatcgag atccgcatca tcgaaaacga
     2521 tcagcggtga tttaccgccc agttccatgg tcacttcttt cagggaagag gccgccgagt
     2581 tagccatcac ttttttgccg ctggcgacac cgccggtaaa tgacactttg gcaatgcccg
     2641 gatgctcggt cagatattgc ccggtctccg cgcccacgcc cggcaacacg ttaaatacgc
     2701 cgtccggcag gcccgcttcg ctgtaaattt cagccagctt taacgcggta agcggggtaa
     2761 cttcgctcgg tttgaaaatc attgcgttgc ctgccgccag cgccggggcg gatttccaca
     2821 gggcaatctg gatcgggtag ttccatgcgc caatccctgc cactacgccc agcggttcgc
     2881 ggcgggtata cacaaaggac gtttcacgca acgggatctg gctgccttcc agcgccggga
     2941 tcagcccggc gtagtactcc agcacgtccg caccggtaac gatatcgacg gttgaggttt
     3001 ccgaatatgc ttttccggtg tcgagggttt ccagttttgc gagttcgtca ttgcgttcac
     3061 gcagaatatc aacggcccga cgcagaatac gcgagcgctc catggcggtc atcgacgccc
     3121 agattttttg cccctgcagg gcgcttttca cggcgcgatc gacatcctcg cgcccggcgg
     3181 cctgcacggt cgccagcacg ttaccgttgg ccgggttaat ggtctcgaag gtgcgaccgc
     3241 tggtggcgga ggtataacca ccatgtatat aaagctgctg ttctgccatt cgggacatca
     3301 cgtctcctcg gttaatcggt gggtaaatgc tgagtaataa agtgacgggt cagcgagtgt
     3361 gcgagaggtt tatccagcgg tttgccgctc agtgccgcgc gcagccataa tccgtcaatc
     3421 agcgcggcaa ggccgtagcc cgcttcctgt gcctgttcgc gcggcaattc gcggcgaaac
     3481 tcgctcacca gattcgacag caagcggcgg ctgctgacct gctgtaaacg atagagcatc
     3541 ggctgatgca tactgctggc ccagaacgcc agccaggctt tcatcgccgc actgctcacc
     3601 tgagtttcat cgaagtttcc ggcaacaatc gcctgtaatc gctgctctgc actgccctgc
     3661 ggaagtgcat gtaatcgatt caaaaccgcg tcacgtaact gactggtgat gtcgcgcatg
     3721 gttgcttcca gcagaccatt tttgtccctg aaatagtggc tgatgatccc cgtagaaacg
     3781 cctgcacggc gggcgatctg cgcgatcgtt gcatcgtgca tgcccacttc atttattgct
     3841 tccagtgtgg cgtcgatcag ttgtctgcgc cggatcgact gcatccccaa tttgggcatt
     3901 ttcgccactc cattcatcag cgttgtttat ctattaaagc ggttattgat tggacgttca
     3961 atataaaatg tgtcttaatt gttacgaatt tgattttaaa tggtaacaat aacagtgggg
     4021 atactggatg acagaccttt cacacagcag ggaaaaggac aaaatcaatc cggtggtgtt
     4081 ttacacctcc gccggactga ttttgttgtt ttccctgaca acgatcctgt ttcgcgactt
     4141 ctcggccctg tggattggcc gcacgctgga ctgggtgtct aaaaccttcg gttggtacta
     4201 tctgctggcg gcaacgctct atattgtctt tgtggtctgt atcgcttgtt cgcgttttgg
     4261 ttcggtgaag ctcgggccag aacaatccaa accggaattc agcctgctga gttgggcggc
     4321 gatgctgttt gctgccggga tcggtatcga cctgatgttc ttttccgtag ccgaaccggt
     4381 aacgcagtat atgcagccgc cggaaggcgc gggacagacg attgaggccg cgcgtcaggc
     4441 gatggtctgg acgctgtttc actacggctt aaccggctgg tcgatgtatg cgctgatggg
     4501 catggcgctc ggatacttta gctatcgtta taatttgccg ctcaccatcc gctcggcgct
     4561 gtacccgatc ttcggtaaac ggattaacgg acctataggt cactcagtgg atattgcagc
     4621 ggtgatcggc actatcttcg gtattgccac tacgctcggt atcggtgtgg tgcagcttaa
     4681 ctatggcttg agcgtactgt ttgatattcc cgattcgatg gcggcaaaag cggcactgat
     4741 cgccttgtcg gtgataatcg ccacgatctc tgtcacctcc ggtgtcgata agggcattcg
     4801 cgtgttatcg gagcttaatg tcgcgctggc gctgggattg atcctgttcg tattgtttat
     4861 gggcgacact tcgttcctgc ttaatgcgct ggtgctgaat gttggcgact atgtgaatcg
     4921 ctttatgggc atgacgctca acagttttgc cttcgaccgt ccagttgagt ggatgaataa
     4981 ctggacgctc ttcttctggg catggtgggt ggcatggtcg ccgtttgtcg gcttgttcct
     5041 tgcgcgtatc tcgcgtgggc gtaccattcg ccagttcgtg ctgggcacgt tgattattcc
     5101 gtttaccttc acgctgttat ggctctcggt gttcggcaat agcgcgctgt atgaaattat
     5161 ccacggcggc gcggcatttg ccgaggaagc gatggtccat ccggagcgcg gcttctacag
     5221 cctgctggcg cagtatccgg cgtttacctt tagcgcctcc gtcgccacca ttactggcct
     5281 gctgttttat gtgacctcgg cggactccgg tgcgctggtg ctggggaatt tcacctcgca
     5341 gcttaaagat atcaacagcg acgcccccgg ctggctgcgc gtcttctggt cggtggcgat
     5401 tggtctgctg acgctcggca tgctgatgac caacgggata tccgcgctgc aaaacaccac
     5461 ggtaatcatg gggctgccgt tcagctttgt gattttcttc gtgatggcgg ggttgtataa
     5521 atctctgaag gtagaagatt accgccgtga aagtgccaac cgcgataccg cgccgcgacc
     5581 gctggggctt caggatcgcc tgagctggaa aaaacgtctc tcgcgcctga tgaattatcc
     5641 gggcacgcgt tacactaaac agatgatgga gacggtctgt tacccggcaa tggaagaagt
     5701 ggcgcaggag ctgcggttgc gcggcgcgta cgtggagcta aaaagcctgc cgccggaaga
     5761 gggacaacag ttggggcatc tggatttgtt ggtgcatatg ggcgaagagc aaaactttgt
     5821 ctatcagatt tggccgcagc aatattcggt gccgggcttt acctaccgcg cacgcagcgg
     5881 taaatcgacc tactaccggc tggaaacctt cctgttagaa ggcagccagg gcaacgacct
     5941 gatggactac agcaaagagc aggtgatcac cgatattctt gaccagtacg agcggcacct
     6001 taactttatt catctccatc gtgaagcgcc gggccatagc gtgatgttcc cggacgcgtg
     6061 attgttattg cataaaaccg cgccatgtct gcatatggcg ctgtttctcc tacctcttga
     6121 tacgttatat ctatacggtt aagcccttag tatctattga tgattaccag acaacagata
     6181 ataaaaaaga aaagaactat tgcagcccaa aacctacatt tgggctgttg cgaatgttca
     6241 ataagttt
//
LOCUS       DBANUU010000209         6183 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000209 DBANUU010000000
VERSION     DBANUU010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.212"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            121..1349
                     /locus_tag="ACIE68_004397"
     CDS             join(121..436,436..1349)
                     /locus_tag="ACIE68_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFX5910604.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSPVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWRVSLVSRCLRVSRAQLHVILRRTDDWKDGRRSRHSDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRIYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRS
                     PESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQQASNGLSDNRCLEI"
     gene            1594..2145
                     /gene="sfaX"
                     /locus_tag="ACIE68_004398"
     CDS             1594..2145
                     /gene="sfaX"
                     /locus_tag="ACIE68_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SfaX"
                     /protein_id="HFX5910605.1"
                     /translation="MRACTQTVCFSNMENNEMNNTDTLEKIIRHQKNKDPAYPFQEHL
                     LMQLCIRANKRMQDNISEFLGAYGINHSVYMVLTTLFTAESHCLSPSEISQKLQFTRT
                     NITRITDFLEKTGYVKRTDSREDRRAKKISLTSEGMFFIQRLTLAQSMYLKEIWGYLT
                     HDEQELFEVINKKLLAHLDDVSS"
     gene            complement(2497..3456)
                     /gene="istA"
                     /locus_tag="ACIE68_004399"
                     /pseudo
     CDS             complement(2497..3456)
                     /gene="istA"
                     /locus_tag="ACIE68_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
     gene            complement(3760..4101)
                     /locus_tag="ACIE68_004400"
     CDS             complement(3760..4101)
                     /locus_tag="ACIE68_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HFX5910606.1"
                     /translation="MNFKNIILSTVLSIASCHALAVGNSQIALSNLHVENTPSGTKAI
                     VGYAENKSNSVVKNAYVKFNLLKDGVVIGQTIDTASNLEPGQKWKIQAFINTFNGSPD
                     SFKVTEVKIVN"
     gene            4407..4644
                     /locus_tag="ACIE68_004401"
                     /pseudo
     CDS             4407..4644
                     /locus_tag="ACIE68_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178298.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            complement(4897..5276)
                     /locus_tag="ACIE68_004402"
                     /pseudo
     CDS             complement(4897..5276)
                     /locus_tag="ACIE68_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
BASE COUNT         1680 a         1343 c         1475 g         1685 t
ORIGIN      
        1 tgtagattca atctgtcaat gcaacaccct agactggccc cctgaatctc cagacaacca
       61 atatcactta aataagtgat agtcttaata ctagttttta gactagtcat tggagagcag
      121 atgattgatg tcttaggacc ggagaaacgc agacggcgta ctacacagga aaagatcgct
      181 attgttcagc agagttttga accgggaatg acggtctccc ctgttgcccg gcaacacggt
      241 gtggcagcca gccagttatt tctctggcgc aagcaatacc aggagggaag tcttactgct
      301 gtggccgcag gagaacaggt cgttcctgcc tctgaacttg ctgctgccat gaagcagatt
      361 aaagaactcc agcgcctgct cggcaaaaaa acgatggaaa atgaactcct taaagaagcc
      421 gttgaatatg ggcgtgcaaa aaagtggata gcgcacgcgc ccttattgcc cggggatgga
      481 gagtaagctt agtcagccgt tgtctccggg tgtcgcgtgc gcagttgcac gtcattctca
      541 gacgaaccga tgactggaaa gatggtcgcc gcagccgtca ctcagatgat acggatgtgc
      601 ttctccgtat acaccatgtt atcggagagc tgcccacata tggttatcgt cgggtatggg
      661 cgctgcttcg cagacaggcc gaacttgatg gtatgcctgc gatcaatgcc aaacgtattt
      721 accggatcat gcgccagaat gcgctgttgc ttgagcgaaa acccgctgta ccgccatcga
      781 aacgggcaca taccggcaga gtggctgtga aagaaagtaa tcagcgatgg tgctctgacg
      841 ggttcgagtt ccgctgtgat aacggagaaa aactgcgggt cacgttcgcg ctggactgct
      901 gtgaccgtga ggcactgcac tgggcggtca ctacgggcgg cttcaacagt gaaacagtac
      961 aggacgtcat gctgggagcg gtggaacgcc gcttcggcaa cgagcttccg gcgtctccag
     1021 tagagtggct gacggataat ggttcatgct accgggctaa tgaaacacgg cagtttgccc
     1081 ggatgttggg gcttgaaccg aagagcacgg cggtgcggag tccggagagt aacggcatag
     1141 cagagagctt cgtgaaaacg ataaagcgtg actacatcag tgtcatgccc aaaccagacg
     1201 ggttaacggc agcaaagaac cttgcagagg cgttcgagca ttataacgaa tggcatccgc
     1261 atagtgcact gggttatcgc tcgccacggg aatatctacg gcagcaagcc agtaatgggt
     1321 taagtgataa caggtgtctg gaaatatagg ggcaaatcca gtggatatct gggatgcccc
     1381 gggaagtcac ctttcagacg ggcggagggc tggtgaatta tccgcgatta ctgagcagta
     1441 tggataatcc tttttcacag acttgtcagc agccagcatt tatgttcttt tatctgaggg
     1501 aatttatctg tctgctgtgt cggaatatct cagttataca gaaatcaggc aggaataaat
     1561 tgtagtggaa agtcgatgtt taccggatga ctgatgcgcg cttgtactca gacagtgtgt
     1621 ttcagtaata tggagaataa tgaaatgaat aacacagaca cattagaaaa aataatcaga
     1681 caccaaaaaa acaaagaccc cgcatatccc tttcaggagc atttgttgat gcaactctgt
     1741 attcgcgcaa ataaaaggat gcaggataat atatctgaat ttctgggggc gtatggaata
     1801 aatcactcag tgtacatggt tctcaccaca ttattcacag cggagagtca ttgtctgtca
     1861 ccttcagaga taagccagaa actgcagttt accagaacta atattacccg cattacagat
     1921 tttttagaaa aaaccggata tgtaaaaagg acggatagca gggaggatcg ccgtgctaaa
     1981 aaaatcagtc tgacatctga aggtatgttt tttattcaga ggctcactct tgcacaaagc
     2041 atgtatctga aagaaatctg gggttatctg acccatgatg aacaggaact gtttgaagtc
     2101 attaataaaa aattactggc gcatcttgat gatgtcagct cataaagcgt gaaatgcctg
     2161 agtatgccgg atagcttcag gcaaaagaat aatgattctt gcagacgtgt ttttccggat
     2221 acaaaaaaaa tgataaaaat tgcagcgcca ggcatctttc aaagcaggga gacctgtacc
     2281 gcgtcgaaaa tttcagccag ttaatatcat tgtctgaacc aggcacttcg cccgggcagg
     2341 agaagaagcg tggttgcggt catcctgtgt actgaagtcg ttatgaagga aaaatacaaa
     2401 taattgccca ttttctctgt acaatagtaa gcgtaaactg accgccgtat gtagccatta
     2461 agccagtatt ggtaacgtag gtgcccactt tttcaactga agtgcgggtt tatcaagctg
     2521 gatgaagagt tctgcacgtg aataacccaa cttctgcatt attttgttat tcagtctttc
     2581 cagcaactcc cgaatgcgct gattaagcga tgcaaggctg tagaagatct catgcctgat
     2641 tcgggccatg atccagcgtt caacaacctg aacgccaact tcagctttgg ctttatcttt
     2701 cggtttacgt ggccgcgcag gcaaaactgc gacattataa tgctcaagca tctgctggta
     2761 ggtagggtta acgtcaggat catacttaca tgccctggat gtggcgcttt tcagattgtc
     2821 cggaacaaca agttcaggaa cgccacccaa ccactggaag cagcgaacat gactcatcac
     2881 ccagtcttca agctgctgag accaggtggc ctctgcccat gtgtaacttg atgccccgag
     2941 aacagctacg atgacctgag cagttcttat ttctccggtc tcagggtcgg taacgccaac
     3001 ggtaggtcca cagtaatcaa cgaaaagttt ttcgccagct ttatgtacct gacgcattga
     3061 tggtgaagtg gttttgagcc attcacggta catccggcag taatggttat agctgtaaaa
     3121 accgcctgga ttacgctcac agtattcttc ccagagtagc tgcagcgtca cgcatttatt
     3181 acgcagttcc cggtgtactg tagcccagtc aggcagagag tgcttcttca tcttaacctg
     3241 ggtctgaagg aacgcatgtt ttagttttgt atcatcccat cctgtaggta agggccactg
     3301 ctttatgcca agttgagccg cccgattagc atatctggat acaacggaag gggagattgc
     3361 aagactacga ccaatttgtc gatggctgag tccaacaccg tatttaagcc taagaatttc
     3421 tttaagtttt ctcatagaaa ttggaactgt tggcataggt atcctttacc ggaatggcaa
     3481 aagatacaga tcaacacacc tgtgaagttc caataacatt gatggagatc actgaataac
     3541 aaaatgagtc aaaagtgatc tccatcgatg ttattcagcg atctgttcaa atgttattac
     3601 ccgatctcga tggaagttat tgagtgatct cctttcatga aaatacgcat tcggtgtcat
     3661 aagcaacgag agtatttgat tcctttatgg cggcctggtg cagagctgcc ctttcccagg
     3721 acctccatat aatttttgta gcggcagtca gtggcgcact cagttaacta ttttcacttc
     3781 agtaactttg aatgagtcgg ggctgccgtt aaaggtgtta atgaaggctt gtattttcca
     3841 cttctggcct ggttcaagat tggatgctgt gtcgattgtt tgaccgataa ctactccatc
     3901 ttttaaaaga ttaaatttta cataagcatt tttgacaaca gagtttgatt tattttcagc
     3961 ataacccaca attgccttcg tcccacttgg ggtgttttcc acatgaaggt tagatagcgc
     4021 tatttgagaa ttacctacag ccagggcatg acaactagcg attgataaaa cagtacttaa
     4081 aattatattt ttaaaattca tattcacctc aagttagtcg ttaatcgagc tggttatttt
     4141 aacgcctcag ataaccttgt tgaatagttt ttcatcgtca gaaaattttc agctttgtaa
     4201 atagagttta tcgccgcccc gtcaccctgt cttacgtatc ctgtacgcac tgtctaattc
     4261 taagggaggt gcgaatgctt tctgttccag catcatcctg tcgtctggtc ggtactgtgg
     4321 ggatgctgtt tcttttcacc cttgccatac ccgttcaggc ggctgaacgc cgaggcttag
     4381 gtcgagcagg gtagtagagt ggttttttta acggctggac acttgatccc ggaccgttac
     4441 gtgctggttg atgctcatca acagcctgta gatttcactc aaggtgaata tgggttactg
     4501 ctggcgttgg ctcaaaactc ccgacgagtc ttaatctgtg agcaattgct ggatttgatc
     4561 cacggtgaga ccactgaggt atttgatcgc accatcgatg tattgataat gcgtctgtgt
     4621 cgcaaaatag agatcaatcc ccattggtgt tttgcaagtc cgatacaacg gttagccagc
     4681 ttctggtcga acttgagctg aaggatctta aaacaggtat ccatagtgct cctggatatt
     4741 ctctttttgg acattgacgt ctccattggt ttaaacacgg caatggagac tgcggtgaaa
     4801 agagttaatt cccggagtga ctggctggat gccaatcaat gatcggaagc atgccaaact
     4861 gtgaacggag atggatgccg ccaaatcata atcgattcag atgccatatt tgcaatatct
     4921 cgttaatcgt ctgttcagcc gggatatatt agcgggccag ggatccggga aaatgccacc
     4981 tgattgccac cagacaattt tttcttcaga aagaaaacag ttaattaagg cggaggtaaa
     5041 atcatggact tcatcagaac gtcctataat cgttaaatgg catctcctgt ctccaaaccg
     5101 ccccagagtt tctgcaaagc agagcgctct tcgagggtca ggtgattatc aggggctgcc
     5161 aatacaccgg aattccagta gctgataaac tccagattga tgcttcctgc ggcctggttc
     5221 cagagcagag ccatgtcagg tcttcctggt agccagaaaa agcctttatt acgataatta
     5281 cgtagcgttc agtgatgatc tgctaataat attctgtcga tccgctaagt gtcagtgtat
     5341 gcggtaagcc cgcccttgag tcactatttg gcaactgcta catctaaggt gacgttattc
     5401 gctggcgccg atattttctc gatgggagat aaccctgcac actatgcagt gtaatactcg
     5461 agctactgca aggttacgca attcagtctt tactgccgtt cttacattcg atttgggtat
     5521 gaaggctttc atcgttttaa cacctttctg caattccatt gttctgagag ctttccaccg
     5581 ctgttgtaca aggtacatgg cacagttgtg tcattaactt ctgtatttcg tcagtgagaa
     5641 cccggtgatt acgtagttca tcttttgtcc agtggattgg ttgccgtata aaggatctta
     5701 tcaacaaagc acagttcctc tatgtcagcg acgacgtagt gctatggctc tttcccaaac
     5761 gcccatttct gactgttcct gcaacgccgg ttagctgtca ctatttttat ggattcgata
     5821 aaaaatcttc atcatgcatg atcgattttg tcgatcgatt cgatcgaatt ttctcttttt
     5881 accctgcctt ttgcatggtt ttaaaatagg caaaattact tgtcattcgt ggaaaatgat
     5941 ggcggagata actgagctta aaaagtttct cttgtctccg tcacttgcca taccagccag
     6001 gaaaagaaga tgtcagcaca agaaaaaaca gtacagattg attcttttga tgcgaaattt
     6061 ttagatataa atacattaag caagcttatc tatgaacgta agagttttgt gatagaaaat
     6121 gtttccgatg ttagtgaaac ggtaaaaaga gtggagcatg aaatagagaa aaggaaatta
     6181 agt
//
LOCUS       DBANUU010000210         6152 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000210 DBANUU010000000
VERSION     DBANUU010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.213"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            89..865
                     /gene="exuR"
                     /locus_tag="ACIE68_004403"
     CDS             89..865
                     /gene="exuR"
                     /locus_tag="ACIE68_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFX5910607.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1105..1188
                     /gene="yqiM"
                     /locus_tag="ACIE68_004404"
     CDS             1105..1188
                     /gene="yqiM"
                     /locus_tag="ACIE68_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFX5910608.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1210..1872
                     /gene="yqjA"
                     /locus_tag="ACIE68_004405"
     CDS             1210..1872
                     /gene="yqjA"
                     /locus_tag="ACIE68_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFX5910609.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1876..2259
                     /gene="mzrA"
                     /locus_tag="ACIE68_004406"
     CDS             1876..2259
                     /gene="mzrA"
                     /locus_tag="ACIE68_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFX5910610.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2406..2774
                     /gene="yqjC"
                     /locus_tag="ACIE68_004407"
     CDS             2406..2774
                     /gene="yqjC"
                     /locus_tag="ACIE68_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFX5910611.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2812..3117
                     /gene="yqjD"
                     /locus_tag="ACIE68_004408"
     CDS             2812..3117
                     /gene="yqjD"
                     /locus_tag="ACIE68_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFX5910612.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3120..3524
                     /gene="yqjE"
                     /locus_tag="ACIE68_004409"
     CDS             3120..3524
                     /gene="yqjE"
                     /locus_tag="ACIE68_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFX5910613.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3514..3813
                     /gene="yqjK"
                     /locus_tag="ACIE68_004410"
     CDS             3514..3813
                     /gene="yqjK"
                     /locus_tag="ACIE68_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFX5910614.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3999..4391
                     /gene="yqjF"
                     /locus_tag="ACIE68_004411"
     CDS             3999..4391
                     /gene="yqjF"
                     /locus_tag="ACIE68_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFX5910615.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4461..5447
                     /gene="yqjG"
                     /locus_tag="ACIE68_004412"
     CDS             4461..5447
                     /gene="yqjG"
                     /locus_tag="ACIE68_004412"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFX5910616.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQNEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DTLGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQEAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5740..6105
                     /gene="yhaH"
                     /locus_tag="ACIE68_004413"
     CDS             5740..6105
                     /gene="yhaH"
                     /locus_tag="ACIE68_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFX5910617.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1544 a         1514 c         1549 g         1545 t
ORIGIN      
        1 caatcaccaa atttatatat gaccaatggc atacccatga ataaagtggt atgataactt
       61 cgtccgcatt ccactccggg agacgcatat ggaaatcact gaaccacgcc gtttgtatca
      121 acaacttgcc gctgacctga aagagcgcat cgaacagggc gtctatctgg taggtgataa
      181 actgcctgca gaacgcttta ttgccgatga aaagaacgtc agccgtacgg tagttcgtga
      241 agccatcatc atgctggaag ttgaaggcta tgtcgaagtg cgtaaaggtt caggtattca
      301 tgtggtttcc aaccagccgc gccatcagca ggcagctgac aataatatgg aattcgccaa
      361 ttacggtccg tttgagttgc ttcaggctcg ccagctcatc gaaagtaata ttgccgaatt
      421 cgcggcaact caggtaacga aacaggacat catgaaactg atggccattc aggaacaggc
      481 gcgcggcgaa caatgcttcc gtgattccga gtgggattta cagttccaca ttcaggtagc
      541 cctggcgacg cagaactccg ccctggcggc catcgttgaa aaaatgtgga cacaacgtag
      601 ccataacccg tactggaaaa aactgcacga acacattgat tcccgtaccg tcgataactg
      661 gtgtgatgac cacgatcaaa tcctcaaggc gctgattcgc aaagatcctc atgctgctaa
      721 gctggcaatg tggcagcacc tggaaaacac caagatcatg ttattcaacg aaaccagcga
      781 cgacttcgag ttcaatgccg accgctatct attcgccgaa aacccggtgg tgcatctcga
      841 tacggccacc agtggcagta aatgaaatac ctgtccgaca ggcgctgcga cgcgcctgtt
      901 tgtctcgtgg gtaagcgaaa agtataaagt gtcagcctgt gtaaatcctc tcgccaccct
      961 cccctgcatt cagcaaaatc agactccacg gacatgtaat tttgataacg aacaacgttg
     1021 acctttgtta caattagatt caatttgaat ttatgttttt gaatgctttc ttatctcacg
     1081 atttaacagg gaatagttca ggctgtgttg atgtatcaaa cccgcagaac ataccaaaac
     1141 agcaataaca ttgcggtagt gcatctttta aaaccagcgt ggcgttaacc gattcaccag
     1201 gaataatgaa tggaactttt gacccaattg ctgcaagccc tgtgggcgca ggattttgaa
     1261 accctggcca atccatcgat gattggcatg ttgtattttg tcttgtttgt aattttgttc
     1321 cttgaaaacg gcttgcttcc agcggccttt ttaccgggcg acagtttact ggtattggtc
     1381 ggcgtgttga ttgcgaaagg cgcgatgggt tacccgcaaa cgattctgct gctgaccgtt
     1441 gccgccagcc tcggctgctg ggtcagctat attcaggggc gatggctggg caatacccgc
     1501 accgtacaaa actggctatc tcatttaccc gcgcattatc atcaacgcgc acaccatctt
     1561 tttcataaac acggtttatc ggcgctgtta attggtcgct ttattgcgtt tgtcagaaca
     1621 ctgctgccga cgattgccgg gttatcaggg ctgaataacg cgcgctttca gtttttcaac
     1681 tggatgagcg gtctgctgtg ggtattgatc ctgacaactc tgggttacat gctcggcaaa
     1741 acgccggtat ttttaaagta cgaggaccag ctgatgtcat gcctgatgct gctcccggtg
     1801 gtgctgctgg tgtttggcct ggcaggttct ctggtcgtgt tatggaaaaa gaaatatgga
     1861 aatcgggggt aagggatgca aatacctcgc atgtcgcttc gccagctagc ctggtccggc
     1921 actgttttac ttctggtcgg cacgctgttg ctggcctggt ccgcggttcg ccagcaagag
     1981 tctacgctgg cgattcgtgc cgttcatcaa ggcacaacga tgccagacgg tttttcgatc
     2041 tggcatcacc ttgacgctca tggcattcct ttcaaaagta tcacccccaa aaacgacact
     2101 ctgttaatta catttgactc cagcgaccag agcgccgccg caaaagcggt cctcgacaga
     2161 acattgcccc atggctacat cattgcgcag caggacaata acagtcaggc tatgcagtgg
     2221 ctgacccggt tacgggataa ctctcatcgc ttcggataac ttcctgttat ccgaaacata
     2281 tcactcactt tggtgatttc accgtaactg tctatgatta atgaggcggt ggttaacact
     2341 accgcttcgt tgtctggaca atcgttcctt tgtaataggt ccgaatcaca atggaaggtt
     2401 caagaatgaa ataccgcatc gctttagctg tttctctctt tgctcttagt gccggtagtt
     2461 atgccactac cctgtgtcag gaaaaggagc aaaatatcct taaggagatc agctatgccg
     2521 aaaaacacca aaaccagaat cgtattgacg gtctgaataa agccctgagt gaagtccggg
     2581 ccaactgttc agatagccag ctgcgtgccg atcatcacaa gaaaatcgca aagcagaaag
     2641 atgaggtggc ggaacgccag caagatttag ccgaggcgaa gcaaaaaggc gatgccgata
     2701 agattgccaa acgcgaacgg aaactggcag aagcgcagga agagctgaaa aagctggaag
     2761 cgcgcgacta ctaactcaca atagtcacta cttactcacc tggagaaaac tatgtcgaaa
     2821 gaacacacta cggaacatct gcgtgctgag ttgaaatccc tttccgatac gctggaagag
     2881 gtgcttagct catctggcga gaagtcgaaa gaagagttga gtaagattcg tagcaaagcg
     2941 gagcaggcac tgaaacagag ccgttatcgc ctgggtgaaa ccggtgatgc catcgccaaa
     3001 caaacccgtg tcgcggcggc gcgtgctgat gagtatgtgc gtgaaaatcc gtggaccggc
     3061 gtgggaattg gcgctgcaat cggtgtagtg ctcggcgttc tgctgtcgcg tcgttaatta
     3121 tggcggacac tcatcacgcg caagggcccg gtaaaagcgt tctgggcatc gggcagcgaa
     3181 ttgtttccat catggttgaa atggtggaaa cacgtctgcg gctggcggtg gtggagctgg
     3241 aagaggaaaa agctaatctc tttcaacttt tactgatgct gggcctgacg atgcttttcg
     3301 ctgcatttgg tcttatgagc ctgatggtgc taattatttg ggcggttgat ccgcaatatc
     3361 gcctgaatgc gatgattgcc accaccgtgg tgttgctgct actggcacta attggcggta
     3421 tctggacgtt acgtaaatcg cgtaaatcca ccttgctgcg ccatacacgc catgagctgg
     3481 caaacgatcg gcaactgctc gaggaggagt cccgtgagca gtaaagtcga acgtgaacga
     3541 cgtaaggcgc aactgcttag ccagatccag caacaacggc tggatctttc cgccagtcgt
     3601 cgtgaatggc tggaggcaac aggcgcttac gatcgtcgct ggaatatgct gctaagtctg
     3661 cgctcctggg cgctggttgg cagtagcgtg atggcgatct ggacgattcg ccatcctaat
     3721 atgctggtcc gctgggccag acgcggtttt ggcgtatgga gcgcctggcg tctggttaaa
     3781 acgaccctca agcagcaaca gcttcgcggt taacttccct ctggccggag caattccggc
     3841 cttatccatc aaattttttg aagatttttg acagttttcc ttgctaacaa tcatcattca
     3901 ccacgtttat gattctctcc atcgacagca acgacgctaa taccgcgccc ttgcacaaaa
     3961 aaacaatcag cagcctgagt ggcgcagtgg agagtatgat gaaaaaatta gaagatgttg
     4021 gtgtactggt agcgcgcatt ttaatgccga ttctgtttat taccgcaggc tggggaaaaa
     4081 tcaccgctta ctctggtacc caacaatata tggaagcaat gggtgtcccg ggttttatgc
     4141 tgccgctggt gattctgctt gagttcggtg gtggtctggc aatcctgttc ggtttcctga
     4201 ctcgcaccac agccctgttt actgcgggct ttacgctgct gacggcattt ttatttcaca
     4261 gcaactttgc tgaaggcgtc aactcgctga tgttcatgaa aaacctgaca atttctggcg
     4321 gattcctgct gctggcaatt accggtccgg gcgcgtatag catcgaccgc ctgctgaata
     4381 aaaagtggta agcacgcgct atacttaacg ataaaaaacg aggaggaagc tcctcgtttt
     4441 tgctattgga ggagagaaaa atgggtcaac tgattgacgg cgtctggcat gacacctggt
     4501 acgataccaa atctaccggc ggtaaatttc aacgttcagc ttccgcattt cgtaactggc
     4561 tcactgccga tggcgcacct ggccccactg gcacaggcgg ttttatcgca gagaaagatc
     4621 gttatcatct ctatgtttca ctcgcctgcc cgtgggcgca ccgcacgctg atcatgcgca
     4681 aactcaaagg actggaaccg tttatttccg tttccgtagt gaacccgctg atgctggaaa
     4741 acggctggac ctttgatgac agttttccgg gagcaaccgg agatacgctc tatcaaaatg
     4801 aatttctgta tcagctttat ctccacgccg atccacacta cagcggacga gttactgttc
     4861 ccgtgctgtg ggacaaaaag aaccacacca tcgtcagcaa cgaatcagca gaaatcatcc
     4921 gcatgttcaa taccgcgttt gatacgctgg gcgcgaaagc gggtgattac tacccaccag
     4981 ccctgcaaac gaaaattgac gaacttaacg gctggattta tgacaccgtt aacaacggcg
     5041 tgtataaagc tggttttgcc accagccagg aagcttacga cgaggcggtg gcgaaagtgt
     5101 ttgaatcgct ggcgcgactg gagcagattt taggccagca tcgttacctg accggcaatc
     5161 agctaaccga agccgatatt cgcctgtgga ccacgctggt gcgttttgat ccggtgtatg
     5221 tgacccactt caagtgtgat aagcaccgca tcagcgatta ccttaatctg tatggcttcc
     5281 tgcgtgatat ctaccagatg ccgggaattg ccgaaacagt caatttcgat cacatccgta
     5341 atcattactt ccgcagccat aagaccatca accctacggg gattatttca attggtccat
     5401 ggcaggatct cgatgaaccg catggacgag atgttcgctt cggttaaaaa aaggcacctt
     5461 acgaggtgcc tctgataatt agctaagtgc atgttgttca tgtcggatgc ggcgtgaacg
     5521 ccttatcaaa cctacaaaac atttatattc aatatgttgc aatgaccgcg caggccagat
     5581 aagcgtagcg catctggcga ttttgcgctt gtcatcagtc ttagcgcgcc ttcacagcac
     5641 tttattaatt ccttgaacga atatttactg ccatttcatt cacgtctatt cttaatttgc
     5701 tgctttaaaa acaagtgatt gagcaaaatt gaggcaaaaa tggactggta tctgaaagta
     5761 ctaaaaaatt atgtcggttt ccggggtcgt gcgcggcgca aagagtactg gatgtttatt
     5821 ctggtcaaca tcatctttac gttcgtgctg gggctgctgg ataaaatgtt aggctggcaa
     5881 cgcgctggtg gcgaaggcat cctgacgaca atctacggta ttctggtgtt tttaccgtgg
     5941 tgggcagttc agttccgccg cctgcacgac accgaccgct cggcgtggtg ggcactgttg
     6001 ttcttaatcc cgtttatcgg ctggcttatt atcatcgtct ttaactgcca ggcgggtacg
     6061 ccaggcgaaa accgctttgg accagatccg aaactggaac cttaaaacaa atctgctcgc
     6121 cggatgtctg actcatccgg cttttatcat ca
//
LOCUS       DBANUU010000211         6133 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000211 DBANUU010000000
VERSION     DBANUU010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.214"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            3..401
                     /locus_tag="ACIE68_004414"
     CDS             3..401
                     /locus_tag="ACIE68_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910618.1"
                     /translation="MILTGALLSLFALVGCKPTDEKAIALGQQAIADDMKDPTSVMFR
                     KDKFVRTDHDDGSVTGFVCGELNAKNGFGAYVGYHSYVVELEMKPKGMFSKGVVYKIL
                     SKSLAPNDPHDLQRYKQVYRKMCNSEAVAH"
     gene            527..1309
                     /locus_tag="ACIE68_004415"
     CDS             527..1309
                     /locus_tag="ACIE68_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910619.1"
                     /translation="MKEEIKRLACNIIDKTGLEISESNRLDIIEKAVNTAMDHIATRL
                     VEIPLPGLPYLKVKLCVWGEPAHARRSALVVFVRKENLRTLKVQVGAWFDGRVIYTDT
                     IICPPGDEHIEAVIRESIRAMRSLALLEDKQNFEDYLLSVKAEPTLSLKADFVTPTNL
                     LEVLINKGANDAVNLIRESEYSTLWDMCKSQLDLVHIIVDAGKACDGVMAEFAGKMVR
                     IANELPMIEQEAKSYATNHVTELLAPYRLESDQRKMISWGSW"
     gene            1365..1847
                     /locus_tag="ACIE68_004416"
     CDS             1365..1847
                     /locus_tag="ACIE68_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910620.1"
                     /translation="MAVIVKADLSKVPSISGNNGYSLRCEEVKINGESAYCSYSVCQH
                     TILAFKENRLPRTSFQSCATAIKAGKCKALKMMVEEIRKGESLYFEDMTALIKEVEER
                     NKQARTIKRKRDSVTINSMVKKSATSQTAITDVYAALIEETTKETHEQIDQHMEAKQQ
                     "
     gene            1844..3166
                     /locus_tag="ACIE68_004417"
     CDS             1844..3166
                     /locus_tag="ACIE68_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFX5910621.1"
                     /translation="MKKLIALKHKLDEMKAMGTNAKKEALANMDDFEQSMVSLMLNPF
                     IRFGVKKYKVAEPLSESVPSDEKAIDVLNKLASRELTGNAAIAAVESIVASMCADGQD
                     VFRRFLLKDPKAGVGISLCNKVFENPIPKFEVQLASPYKEKGDKYPFKPNPKAKWPMI
                     GSLKLDGLRVICEVIVDEEEVNFLSRTGNPITSLDHLKPAMLELGKLSGHKHIFFDGE
                     GTAGSFNQSVSALRKKNVQAIGAIYHVFDFFLPEWRAQAKSKEYAKTCMKLKERLAML
                     VALFKNDRSEGYAQDIHLHPFYIIHSHEDFIERFMKRLDDNEEGEMGKDPNSVYEFKR
                     TRSWWKLKDEDSEDGEIIDFEPGDPDSGFANTLGKIVIRLENGVIVRASGIKHKYLDE
                     IWNNKEKYRGRIVEVHCHEKTPDGSLRHPRLKWPRCLRDTEDRIGDKE"
     gene            3163..3372
                     /locus_tag="ACIE68_004418"
     CDS             3163..3372
                     /locus_tag="ACIE68_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910622.1"
                     /translation="MIVLSKREKETLHEISKWPEFPEYWKPKTRAKLERLGLVANVSE
                     TWCSANYQLTDKGKLLLQQLVESGA"
     gene            3524..3814
                     /locus_tag="ACIE68_004419"
     CDS             3524..3814
                     /locus_tag="ACIE68_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type natriuretic protein"
                     /protein_id="HFX5910623.1"
                     /translation="MSNLTSWDWWLATYCLAAGVAFAFYVGQLVVKLLLIKFASHKRI
                     DDGLWRLGTLVETRYGQLKENETITIQAKRFTATITRTPSRRVALIKKVTTE"
     gene            4593..4667
                     /locus_tag="ACIE68_004420"
     tRNA            4593..4667
                     /locus_tag="ACIE68_004420"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4625..4627,aa:Asn,seq:gtt)
     gene            4673..4759
                     /locus_tag="ACIE68_004421"
     tRNA            4673..4759
                     /locus_tag="ACIE68_004421"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4707..4709,aa:Tyr,seq:gta)
     gene            4764..4839
                     /locus_tag="ACIE68_004422"
     tRNA            4764..4839
                     /locus_tag="ACIE68_004422"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4797..4799,aa:Thr,seq:tgt)
     gene            5053..5139
                     /locus_tag="ACIE68_004423"
     tRNA            5053..5139
                     /locus_tag="ACIE68_004423"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5085..5087,aa:Ser,seq:tga)
     gene            complement(5319..>6133)
                     /locus_tag="ACIE68_004424"
     CDS             complement(5319..>6133)
                     /locus_tag="ACIE68_004424"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910624.1"
                     /translation="YSPSSADYEKRAYIEQLRNSISPNVSLLIATFGLNIDTNKTYDA
                     KRLYSLLSKYNFFEGQLFFEEAISKVHYLDSYAAVIFDNEYRKDVEFYVEEMVRGRET
                     PKIDSRYRHLRATFAILWSYNNPCRQHLLQRFNDLPMHMRNSIELRMENAADKVAEFN
                     SLLLGFIGWELRIAGNKVRVIKDEKHLKRLIKLNDKYPIPPREKGILLTNGFTNRFAD
                     EIKNSLSMYKLENAYLALNSNQHKEKVIDEIVVEVEKIVDKFKAELDSLDFN"
BASE COUNT         1793 a         1263 c         1443 g         1634 t
ORIGIN      
        1 agctgatttt aacaggagct ttattgtctt tgtttgcttt agtggggtgt aagcctacag
       61 acgagaaggc tattgctctg ggccaacaag caattgcgga tgatatgaaa gatcccacgt
      121 cggtgatgtt taggaaagat aagtttgtca ggactgatca tgacgatggg agtgtgacag
      181 gctttgtgtg tggtgaatta aacgccaaaa atggctttgg tgcatacgtg ggctatcact
      241 catatgttgt tgaactcgaa atgaagccaa agggcatgtt ttcaaaaggg gtggtataca
      301 aaatcctatc gaagtcgttg gcacctaatg atcctcatga tttacagcgt tataagcagg
      361 tctacagaaa gatgtgcaac agtgaggctg tagctcatta aaggtgaatg ctgggctgag
      421 gatatgcccg gcatcttagt ataaacaact attgcaccta aaatgataag tagatacata
      481 cttaagtttt tgtaagaata ctttcatccg ttagctagga gttggtatga aagaagaaat
      541 taagagatta gcctgcaaca tcattgataa aactggttta gaaatcagcg agagcaatcg
      601 gctagacatc attgaaaaag ccgtcaatac ggcaatggat catatcgcca ctcgtttggt
      661 cgagatccct ctaccggggc taccttatct gaaggttaag ttgtgcgtat ggggcgaacc
      721 tgcacatgca cgacgttctg cattagttgt ttttgtacgc aaagaaaact tacgaactct
      781 aaaagtgcag gttggggcgt ggtttgatgg cagagtcatc tatacagata ccattatctg
      841 tcctccaggt gacgaacata ttgaagccgt tattcgagaa tcaattcgag ctatgcgtag
      901 tttagctttg ctggaagaca agcaaaactt tgaagattac ttgctgtcgg taaaagctga
      961 accaacattg tctctgaaag ccgatttcgt caccccgaca aatctgttgg aggttttgat
     1021 taataaaggg gctaatgatg ctgtaaacct aatcagggag agcgaatatt cgactctttg
     1081 ggacatgtgc aaaagccagt tggatcttgt gcatatcatt gttgatgcgg gtaaggcgtg
     1141 tgatggcgta atggcggaat ttgctgggaa gatggtcagg attgctaacg aattaccgat
     1201 gatagagcaa gaggccaaat catacgccac caatcatgtc acagagcttc ttgcccccta
     1261 tcgcttagaa agcgatcagc gcaagatgat tagctgggga agttggtaag ctctctgtgt
     1321 gtcgtttttt acgcaaataa tgataggtaa gcacaagatt atttctggcg gtaattgtga
     1381 aagctgattt gtcaaaagtc ccttctattt caggaaataa cggttattca cttcgttgtg
     1441 aggaagtaaa gataaacggt gagtcggcat attgcagcta ctccgtttgc cagcacacca
     1501 ttcttgcctt caaagaaaac cgtcttcctc gaacgtcttt ccagtcgtgc gcaaccgcta
     1561 ttaaagcagg caaatgcaag gcgttaaaaa tgatggttga agagattcgt aaaggagaat
     1621 ctctgtattt cgaagatatg accgcactca ttaaggaggt tgaagaacgg aataaacaag
     1681 ccagaactat aaaacgaaaa cgtgacagtg taacgattaa tagcatggtt aagaagagcg
     1741 ccacatcaca aacagcgatc actgacgtgt atgcggcgtt gattgaagaa acaacaaaag
     1801 aaacacatga gcaaatcgat caacatatgg aggcaaaaca acaatgaaaa agttgatcgc
     1861 acttaagcat aagctggacg aaatgaaagc tatgggaacc aatgcaaaaa aagaggcatt
     1921 ggccaacatg gatgactttg aacaaagcat ggtttcattg atgctcaacc ctttcatccg
     1981 ttttggggta aagaaataca aagtggcaga gccgcttagt gagtccgtcc caagtgacga
     2041 aaaagccatt gatgtactga ataagctggc ctctcgcgag ctgacgggga acgcagcaat
     2101 agctgctgtt gagtctatcg tggcgtcaat gtgcgccgat gggcaggacg tgttccgtcg
     2161 tttcctctta aaagacccga aagcgggtgt tgggattagc ctatgcaaca aggtttttga
     2221 aaatcccatt ccgaaattcg aggtgcagct ggcgtcaccg tataaagaaa aaggcgacaa
     2281 ataccccttc aagccaaacc ctaaagcaaa atggccgatg attggcagtc ttaagctcga
     2341 tggtttgcga gtaatttgcg aggttattgt tgacgaggaa gaggttaact tcctttctcg
     2401 tactggtaat ccaatcacgt ctctcgatca cctaaagcca gcaatgctcg aattaggcaa
     2461 actttcaggc cacaaacaca tcttcttcga tggtgaagga acagccggtt catttaacca
     2521 gtccgtatct gcattgcgca aaaagaacgt gcaggcaatt ggcgctattt atcatgtttt
     2581 cgacttcttc ctaccggaat ggcgagcaca ggctaaatcc aaagagtatg caaagacatg
     2641 tatgaagctg aaagagcgcc tggctatgct cgtggcgttg ttcaaaaacg atcgcagtga
     2701 aggctacgca caagacattc acctgcatcc gttctacatc atccatagcc acgaagactt
     2761 catcgaacgc ttcatgaaac gcctggacga taacgaagaa ggggagatgg gcaaagatcc
     2821 gaactctgtt tacgagttta aacgtacccg cagctggtgg aagttaaaag acgaagattc
     2881 agaagatggt gaaattatcg attttgagcc gggcgacccg gactctggtt ttgccaacac
     2941 gcttggaaaa attgttattc gtcttgaaaa cggcgtcatc gttcgtgcga gcggcattaa
     3001 gcataaatat ctggacgaga tctggaacaa caaagagaag taccgtggtc gtattgtcga
     3061 ggttcattgt cacgagaaaa cacctgatgg cagcttacgc cacccacgac tgaaatggcc
     3121 tcgttgctta cgcgataccg aagatcgaat cggagataaa gaatgatcgt attaagtaaa
     3181 cgggagaaag aaacgcttca tgaaataagt aagtggccgg agttccctga gtactggaag
     3241 cctaaaacgc gagctaagtt agagcgttta gggttggttg caaacgtttc tgaaacgtgg
     3301 tgctcggcca actaccagtt aactgataaa gggaaattat tgctacagca attagtagaa
     3361 tcaggagcgt aaaaatgatt ccatacatct cattagcttt tatgggtggc ttccttatcg
     3421 gcttcggcat ctgtcgtgat ttaattaagc aggaacttaa aaccaaaaca ctgtgcatcg
     3481 gaaagcgtgt gtatcgggta gttcatgaaa caaaggttag aaaatgagca atttaacttc
     3541 ttgggactgg tggttggcca cctattgctt agcggccgga gtcgcattcg ccttttacgt
     3601 aggtcagtta gtcgtaaaac tgttgctgat taaatttgct agtcataaac gtatcgatga
     3661 tggtctgtgg cgtcttggca ccctggtgga aactcgctac gggcaactta aggagaacga
     3721 aaccattact atccaagcga aacgattcac tgccaccatc acaagaacac ctagtcgtag
     3781 agtggccttg atcaaaaaag tcacaaccga ataaaaacac aataataagt atttacttac
     3841 ttatctttta tgtataagat gactttgttt tcgttgagac gcgactgttt gaacttaaat
     3901 acaagtgcaa acgaagaagt ctatctggca gtagcctaat aagccaaaca ccagcgaggt
     3961 cagtttccag cctcgttacc gaaatgggac acactgagcg agtgtgattg cagaacgcag
     4021 gagggaacat gagtgttccc tccgatgaag taacagaatg ggcggtcggt atattttcaa
     4081 ctccatatga ctcccggatt cttagcctct gaccgcccat cctgttacgt cattttgttc
     4141 aattatgtcg tttatactgg gttaaaaagc ggcgacgtag cccggctggt atggttagcc
     4201 agcacacaac gttgaggcca ttacattttt atcaattcta aggttctatt cacagagata
     4261 ccggcgagcg ttgatatgta acatgttggg caaacattca atcggagtag tggcctcaac
     4321 gttgtgaaga caggattgtt gtgtaggttt aaccactgtt gccattggtg cctgttttca
     4381 caacaaatga ttccatacat cacattgtat aaattacaaa gtaggtgctg tcctcagaaa
     4441 catcatctac ttaaagattt tgccttctac tattgagcga agtcgaaagc gtctggcact
     4501 aacgaaaagt gcaagtagcg gtgcgtttcc tggcagaaac taaaccgtcg cgattggcac
     4561 tgttgagtaa taaatactgg cagtgccgaa ttgatggtgt agctcagcgg tagagcagtt
     4621 ggctgttaac caactggtcg gtggttcgaa tccacccacc atcgccaatt taggggagtt
     4681 agtccgtaga ggtagcggtg tagactgtaa atctactgtc attgcgactc gggtggttcg
     4741 actccatcac tccccaccaa attgccggtt tagctcagtt ggtagagcag tcgccttgta
     4801 agcgaatggt cggcggttcg aatccgttaa ccggcaccaa cacaacagga aagagcattg
     4861 agaaccgccg agactgccct gttttaagcg taagtccgtg caaacgtcat acagtgctct
     4921 tttcgttgtg gtgaatgcgg ctaagcgcac gcggggaaat ggttatattg atcctttgtg
     4981 atgactattg gctccgtcca cggtggataa ccagccaaag gacaccggga agcccccggc
     5041 accacaacaa ctggagagta gggagcatgg tgctcaagcg gtcttgaaaa ccgtcccatt
     5101 gcgcaagcga tgatggttcg attccattac tctccgccag acacagcgtt gagcggtttg
     5161 gccttttaat cacccagatt aagactccgc taacataaac cagaccgctc aacgctgtga
     5221 tagacaattg cggcagacgt tcttaaccat agcttgctaa catcctagca acactttttt
     5281 cagcgcaaat aattaaaggg gcttcggccc ctttttgctt agttgaaatc caaagaatca
     5341 agttctgctt taaatttatc gacaattttc tcaacttcaa caacaatttc atcaatcacc
     5401 ttttctttgt gttgattaga gttgagtgct aaataggcat tttctaactt atacatagac
     5461 agactatttt taatctcatc agcgaatcta ttggtgaaac cattggttaa cagaatacct
     5521 ttctctcggg ggggaatagg atacttatcg ttgagtttta ttagcctctt taaatgtttt
     5581 tcatccttga tcaccctcac tttattacct gcgattctaa gctcccagcc tataaaacca
     5641 agtaacaaag agttgaactc agctacttta tcggctgcat tttccatcct cagctcaatg
     5701 ctgtttcgca tatgcatagg taggtcatta aatctttgaa gcaaatgttg tcggcagggg
     5761 ttgttgtaag accacagaat tgcgaacgta gcccttaaat gacgatatct tgaatcgatt
     5821 ttaggggtct cacgacctcg aaccatttct tcaacataaa actctacatc ttttcggtat
     5881 tcgttatcaa atatcacagc agcataagag tccaaataat gaactttcga tattgcttct
     5941 tcaaaaaaaa gttggccttc gaagaagtta tacttgctta gcaggctgta aagcctcttt
     6001 gcgtcatagg ttttattagt gtcaatatta aggccgaatg tagctatcag caggcttaca
     6061 ttaggactaa tgctgttgcg cagctgttct atgtaagctc tcttttcata atcggctgat
     6121 gacggcgaat aga
//
LOCUS       DBANUU010000212         5968 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000212 DBANUU010000000
VERSION     DBANUU010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.215"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            219..866
                     /gene="fucA"
                     /locus_tag="ACIE68_004425"
     CDS             219..866
                     /gene="fucA"
                     /locus_tag="ACIE68_004425"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFX5910625.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            894..2042
                     /gene="fucO"
                     /locus_tag="ACIE68_004426"
     CDS             894..2042
                     /gene="fucO"
                     /locus_tag="ACIE68_004426"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFX5910626.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2097..2852)
                     /gene="xni"
                     /locus_tag="ACIE68_004427"
     CDS             complement(2097..2852)
                     /gene="xni"
                     /locus_tag="ACIE68_004427"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFX5910627.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(2964..4331)
                     /gene="sdaB"
                     /locus_tag="ACIE68_004428"
     CDS             complement(2964..4331)
                     /gene="sdaB"
                     /locus_tag="ACIE68_004428"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFX5910628.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4389..5678)
                     /gene="sdaC"
                     /locus_tag="ACIE68_004429"
     CDS             complement(4389..5678)
                     /gene="sdaC"
                     /locus_tag="ACIE68_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFX5910629.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1495 a         1479 c         1594 g         1400 t
ORIGIN      
        1 ttttgataaa tatttttaag agcggtcatt tttaatgggg ttataaaaag agaacttgca
       61 gttatttact gtgattagtt gaaccaggtc acaaaatata agcttgtgaa tttctttctt
      121 gttaatcagg aagaagatgg tgggtaatta aacggctaat tcaatagtgt gaaaggaaca
      181 acattattgc cctgttttga atcagagaga ggtagctaat ggaacgaaat aaacttgctc
      241 gtcagattat tgacacttgc ctggaaatga cccgcctggg actgaaccag gggacagcgg
      301 ggaacgtcag tgtacgttat caggatggga tgctgattac gcctacaggc attccatatg
      361 aaaaactgac ggagtcgcat attgtcttta ttgatggcaa cggtaaacat gaggaaggaa
      421 agctcccctc aagcgaatgg cgtttccata tggcggccta tcaaagcaga ccggatgcca
      481 acgcggttgt tcacaatcat gccgttcatt gcacagcagt ttccattctt aaccgaccga
      541 tccccgctat tcactacatg attgcggcgg caggtggtaa ttctattcct tgcgcgcctt
      601 atgcgacctt tggaacacgc gaactttctg aacatgttgc gctggctctc aaaaatcgta
      661 aggcaacttt gttacaacat catgggctta tcgcttgtga ggtgaatctg gaaaaagcgt
      721 tatggctggc gcatgaagtt gaagtgctgg cgcaacttta cctgacgacc ctggcgatta
      781 cggacccggt gccagtgctg agcgatgaag agattgccgt agtgctggag aaattcaaaa
      841 cctatgggtt acgaattgaa gagtaatttc gtaaagcaac aaggagaagg atgatggcta
      901 acagaatgat tctgaacgaa acggcatggt ttggtcgggg tgctgttggg gctttaaccg
      961 atgaggtgaa acgccgtggt tatcagaagg cgctgatcgt caccgataaa acgctggtgc
     1021 aatgcggcgt ggtggcgaaa gtgaccgata agatggatgc tgcagggctg gcatgggcga
     1081 tttacgacgg cgtagtgccc aacccaacaa ttactgtcgt caaagaaggg ctcggtgtat
     1141 tccagaatag cggcgcggat tacctgatcg ctattggtgg tggttctcca caggatactt
     1201 gtaaagcgat tggcattatc agcaacaacc cggagtttgc cgatgtgcgt agcctggaag
     1261 gactttcccc gaccaataaa cccagtgtac cgattctggc aatccccacc acagcaggca
     1321 ctgcggcaga agtgaccatt aactacgtga tcactgacga agaaaaacgg cgcaagtttg
     1381 tttgcgttga tccgcatgat atcccgcagg tggcgtttat tgacgctgac atgatggatg
     1441 gtatgcctcc agcgctgaaa gctgcgacgg gtgtcgatgc gctcactcat gctattgagg
     1501 ggtatattac ccgtggcgcg tgggcgctaa ccgatgcact gcacattaaa gcgattgaaa
     1561 tcattgctgg ggcgctgcga ggatcggttg ctggtgataa ggatgccgga gaagaaatgg
     1621 cgctcgggca gtatgttgcg ggtatgggct tctcgaatgt tgggttaggg ttggtgcatg
     1681 gtatggcgca tccactgggc gcgttttata acactccaca cggtgttgca aacgccatcc
     1741 tgctaccgca tgtcatgcgc tataacgctg actttaccgg tgagaagtac cgcgatatcg
     1801 cgcgcgttat gggcgtgaaa gtggaaggta tgagcctgga agaggcgcgt aatgccgctg
     1861 ttgaagcggt gtttgctctc aaccgtgatg tcggtattcc gccacatttg cgtgatgttg
     1921 gtgtacgcaa ggaagacatt ccggcactgg cgcaggcggc actggatgat gtttgtaccg
     1981 gtggcaaccc gcgtgaagca acgcttgagg atattgtaga gctttaccat accgcctggt
     2041 aaatgcgctg atgtgataat gccggatacg acgtttgcgg cgtatccggc tcgccgttac
     2101 cgtaccaacc gcaattgctg taaattgccg tcgatatgca aatcggtttg taagcgggca
     2161 atatcgcggc acagaaacgc catctcttta tgggtttcta attttttgcg ccacttttcg
     2221 gcaaccgcat ccagattctc atatatccct tccagactct gaaactcgac cagcagctgc
     2281 gtggcgcttt ttggtccgat tcccgcaaca cccggtacct ttgaactgct gatccccgca
     2341 agtccccagt aatcgggcaa ctgctgcggt tgaacgccaa attctttatc gataaatggc
     2401 gcatccagcc aacgtttctg gaagtaatca cgaatacgta atgtcggtga aagtaactga
     2461 cagtagcctt tatctgtcga aacaatcgtt gcctgatgcc cggcctgtgt cactttgacc
     2521 gccagcgtgg cggctaagtc atcggcttcg ttgccgctgg ttgaccagca cgggacgccg
     2581 cgttgctcaa aggcggcgcg taatgcaggc atctcgtcgt gcaactcttc cggcattggc
     2641 ggtcgacccg ctttgtaatc tggtaaacgc tgatgacgcc agccgctact gcggttttca
     2701 tcatcaaaaa cggcgaccgc gtgggttggc tggctgtgca taatgagctg atcgagcgca
     2761 tgctggcagg tttcgacaca gggcgatccc tgaacggcat gaatgcgacg aataagattc
     2821 agtgcatcga caataagcaa atgaacagcc acgataaccc ccttaaagat caggcgataa
     2881 gggtaacatt cgatgccgga acaggctacg gctggatgag aaatcgggaa gaggcctcgc
     2941 aaaacgaggc ctctggagag cgattaatcg caggcaacga tcttcattgc caggccgccg
     3001 cgagaggttt cgcggtactt ggcgttcata tctttacctg tttcgtacat ggtttcgata
     3061 actttatcga ggcagacgcg cggctcgctg gtacggcgta gcgccatacg tgcggcgttc
     3121 accgctttta ctgccgcaat ggcgttacgc tcgatgcatg gcacctggac ctgtccggcg
     3181 accgggtcac acgtcagacc gaggttgtgc tccatggcga tttccgccgc gatgcacacc
     3241 tgcgccgggc ttgcacctaa cagttctgcc agaccagccg ccgccattga gcacgccaca
     3301 ccaacttcac cctggcagcc gacttcagca ccagaaattg acgcgttcat cttataaaga
     3361 gaaccaatgg cactggctac cagcaggtaa cgagccagtg agttagcgtt cacttcgcgg
     3421 ataaacttgt cgtagtacgc cagcactgcc gggataatcc cgcacgcacc gttagtcggc
     3481 gcagtcacca cgcgtccgcc agcagcgttc tcttcgttca ctgccaatgc aaacatgttg
     3541 atccagtcaa caaccgccat cgggtcagtg gtggttttat cctggctgac cagcatccgg
     3601 cgtagtgccg cagcacggcg tggaacgcgc agtttgccag gcaacacgcc ttcggtggaa
     3661 ataccgcgct caataccgcc acgcatgact tcccatacgt tcgccaggtg ctgttccagc
     3721 tcttctttgc tgtgcagcgc cagctcattt ttcatcatca ggccagagag tgacagcccg
     3781 gtttcctgac aatgtttttg cagatcggct gctgaactgt acggataagg aacttcaacc
     3841 ggtgcgctat tctgctggcc aaaatgctct tcatcaacga taaagccacc gccaatagag
     3901 tagtaagtct ggctgtaaac gactttatcg cccgccagcg cggtaatgcg cataccgttt
     3961 tcatgcagag aaaggttgtc tgcatggaag ttcatgcact gatcaaccgg gaactccact
     4021 tcatgctgac cgtttgccag catcaggcga ccatgagtat tcacatcctg aataaaacca
     4081 gggatggaat cgatatccac ggtatccggc aggttacccg ccaggcccat aataatggcg
     4141 atatcagtgt ggtggccttt accggtcaga gagagcgagc cgtacacgtc aaccaccacg
     4201 cgggtcacgt ctttaagcag attacgggca atcagatcgt cggtaaattg tttacccgct
     4261 ttcattggtc caacggtatg agaactggaa gggccaatgc cgattttgaa aatatcgaat
     4321 acgctaatca taggaaatac atcgcgttaa aacggaggaa gcgccgcccg aaagcggcgc
     4381 gaaaggactt agctgaacag agagtagaag attgcggaga ttgcaatcag acccatcacg
     4441 acaacgaata cgttgctgat gtgaccgctg tacttacgca ttgccggtac tttctgaatt
     4501 gcgtacatcg gcatcaggaa caggatcatc gcgatgattg ggccgcccag ggtttcaatc
     4561 atacccagga tgctcgggtt cagggtggca acaatccagg tcgttaccag catgaacagc
     4621 gcagtgatac ggttcagctt gttgatttcg atagacttac ctttaccacg cagagattta
     4681 atcaccatac cgttgaagcc ttcacgtgcg cccaggtagt ggccgaggaa ggatttggtg
     4741 atagcgataa tcgcgataat cggagccatc cacgcgataa ccggtgcgtt aaagtggtta
     4801 gccaggtaag acagaatcga gatgttctgc tctttagccg cagccaggtc tgccggagtc
     4861 aggctcagta cgcagctgaa gacgaagaac attacggtca gcaccatcat gatgtgtgcg
     4921 aatgccagga tcttcgagca tttctgttct gccatatcgc cgtactcttc acgcttcgca
     4981 acggcgaaag aagagatgat cggagagtgg ttgaacgaga acaccattac cggaattgcc
     5041 agccacaggg tcatccacag accgtttccg gttgcagatg cagtgtccag agacagcgtt
     5101 tccagtgctg cgccgttcca ctgcgggatg aggtacagag ccagcagcat cagtacgcca
     5161 acaaacggga ataccagaat actcatcgct ttaacgatca tctgctcacc gaagcgaacg
     5221 atggtcatca tacccacgat caggatcagc gacagaatcg cacgcggcgg tggcgtcata
     5281 cccagctggt gagacatgaa gctttcaacg gtattggtga ttgccacgct ataaaccagc
     5341 aggatcgggt agatagcgaa gaagtagagc agggtaatca gtttacctgc gccaatacca
     5401 aagtgttctt ctacaacctc ggtgatgtct tcgcccgggt ttttaccaga cagtacgaag
     5461 cgagtcaggc cgcggtgagc aaaaaacgtc atcgggaacg caaggatagc catgatgatc
     5521 agcgggatca taccgccaac accggcgttg attggcagga acagcacgcc cgcgccgatt
     5581 gccgtgccgt aaaggcccag catccacatg gtgtctgtct tgcgccaggc actacgagag
     5641 tctttcgacg caatcgtgct ggtttgagtc gtttccatct atttctcctg gaggaatgca
     5701 aataattcag cttttagcca gttcagcccg ggaaggtgcc tgaccgtaaa aatgaaatct
     5761 gtctatcggc gatggcttaa tcatttaccg ccgtaataat ttgagggcgg aaagatacac
     5821 acaagttatg tatctatcag tgatcttgat cccaaataca ataatctact tgtaagatag
     5881 attatcccaa cgctttactc tgtaaatcaa aagtaaaaga gaatttaagg gcaatatttg
     5941 cccattaaac gacatctgac ttcttccg
//
LOCUS       DBANUU010000213         5883 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000213 DBANUU010000000
VERSION     DBANUU010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.216"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            53..1204
                     /gene="yiaY"
                     /locus_tag="ACIE68_004430"
     CDS             53..1204
                     /gene="yiaY"
                     /locus_tag="ACIE68_004430"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFX5910630.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTDKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1369..2907
                     /gene="aldB"
                     /locus_tag="ACIE68_004431"
     CDS             1369..2907
                     /gene="aldB"
                     /locus_tag="ACIE68_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFX5910631.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKHIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3126..3281)
                     /locus_tag="ACIE68_004432"
     CDS             complement(3126..3281)
                     /locus_tag="ACIE68_004432"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910632.1"
                     /translation="MAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRP
                     LTIETRD"
     gene            3452..3775
                     /locus_tag="ACIE68_004433"
     CDS             3452..3775
                     /locus_tag="ACIE68_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFX5910633.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            3781..4917
                     /locus_tag="ACIE68_004434"
     CDS             3781..4917
                     /locus_tag="ACIE68_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFX5910634.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(4914..>5883)
                     /locus_tag="ACIE68_004435"
     CDS             complement(4914..>5883)
                     /locus_tag="ACIE68_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFX5910635.1"
                     /translation="KLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADFES
                     KVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYAPT
                     GIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDESL
                     VASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKE
                     VRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLD
                     LVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         1452 a         1412 c         1538 g         1481 t
ORIGIN      
        1 tgaaaatcct cagtaagctg cccacccttt tttacacttt caggagtgtg ttatggcatc
       61 ttcaactttc tttattcctt ctgtgaatgt catcggcgct gattcattga ctgatgcaat
      121 gaatatgatg gcagattatg gatttacccg taccttaatt gtcactgaca gtatgttaac
      181 gaaattaggt atggcgggcg atgtgcaaaa agcactggaa gaacgcaata tttttagcgt
      241 tatttatgat ggcacccaac ctaaccccac cacggaaaac gtcgccgcag gtttgaaatt
      301 acttaaagag aataattgcg atagcgtgat ctccttaggc ggtggttctc cacacgactg
      361 cgcaaaaggt attgcgctgg tggcagccaa tggcggcgat attcgcgatt acgaaggcgt
      421 tgaccgctct gcaaaaccgc agctgccgat gatcgccatc aataccacgg cgggtacggc
      481 ctctgaaatg acccgtttct gcatcatcac tgacgaagcg cgtcatatca aaatggcgat
      541 tgttgataag cacgtgactc cgctgctttc tgtcaatgac tcctcgctga tgatcggtat
      601 gccgaagtca ctgaccgccg ccactggtat ggacgcctta acgcacgcta tcgaagcgta
      661 tgtttctatt gccgccacgc cgatcactga cgcttgtgca ctgaaagccg tgaccatgat
      721 tgccgaaaac ctgccgttag ccgttgaaga tggcagtaat gcgaaagcgc gtgaagcaat
      781 ggcttatgcc cagttcctcg ccggtatggc gttcaataat gcttctctgg gttatgttca
      841 tgcgatggcg caccagctgg gcggtttcta caacctgcca cacggtgtat gtaacgccgt
      901 tttgctgccg catgttcagg tattcaacag caaagtcgcc gccgcacgtc tgcgtgactg
      961 tgccgctgca atgggcgtga acgtgacaga taaaaacgat gcggaaggtg ctgaagcctg
     1021 cattaacgcc atccgtgaac tggcgaagaa agtggatatc ccggcaggcc tacgcgacct
     1081 gaacgtgaaa gaagaagatt tcgcggttct ggcgactaat gccctgaaag atgcctgtgg
     1141 ttttactaac ccgatccagg caactcacga agaaattgtg gcgatttatc gcgcagcgat
     1201 gtaatcatca tttccacaac ggctggcaaa ttgtcagccg ctttttcaac tatctctgta
     1261 acccttgccc gtaaattcgt gatagctgtc gtaaagctgt taccgactgg cgaagatttc
     1321 gccagtcacg tctacccttg ttatacctca caccgcaagg agacgatcat gaccaataat
     1381 cccccttcag cacagattaa gcccggcgag tatggtttcc ccctcaagtt aaaagcccgc
     1441 tatgacaact ttattggcgg cgaatgggta gcccctgccg acggcgagta ttaccagaat
     1501 ctgacgccgg tgaccgggca gctgctgtgc gaagtggcgt cttcgggcaa acgagacatc
     1561 gatctggcgc tggatgctgc gcacaaagtg aaagataaat gggcgcacac ctcggtgcag
     1621 gatcgcgcgg cgattctgtt taagattgcc gatcgaatgg aacaaaacct cgagctgtta
     1681 gcgacagctg aaacctggga taacggcaaa cccattcgcg aaaccagtgc tgcggatgta
     1741 ccgctggcga ttgaccattt ccgctatttc gcctcgtgta ttcgggcgca ggaaggtggg
     1801 atcagtgaag ttgatagcga aaccgtggcc tatcatttcc atgaaccgtt aggcgtggtg
     1861 gggcagatta tcccgtggaa cttcccgctg ctgatggcga gctggaaaat ggctcccgcg
     1921 ctggcggcgg gcaactgtgt ggtgctgaaa cccgcacgtc ttaccccgct ttctgtactg
     1981 ctgctaatgg aaattgtcgg tgatttactg ccgccgggtg tggtgaacgt ggtcaatggc
     2041 gcaggtgggg aaattggcga atatctggcg acctcgaaac acatcgccaa agtggcgttt
     2101 accggctcaa cggaagtggg ccaacaaatt atgcaatacg caacgcaaaa cattattccg
     2161 gtgacgctgg agttgggcgg taagtcgcca aatatcttct ttgctgatgt gatggatgaa
     2221 gaagatgcct ttttcgataa agcgctggaa ggctttgcac tgtttgcctt taaccagggc
     2281 gaagtttgca cctgtccgag tcgtgcttta gtgcaggaat ctatctacga acgctttatg
     2341 gaacgcgcca tccgccgtgt cgaaagcatt cgcagcggta acccgctcga cagcgtgacg
     2401 caaatgggcg cgcaggtttc tcacgggcaa ctggaaacca tcctcaacta cattgatatc
     2461 ggtaaaaaag agggcgctga cgtgctcaca ggcgggcggc gcaagctgct ggaaggtgaa
     2521 ctgaaagacg gctactacct cgaaccgacg attctgtttg gtcagaacaa tatgcgggtg
     2581 ttccaggagg agatttttgg cccggtgctg gcggtgacca ccttcaaaac gatggaagaa
     2641 gcgctggagc tggcgaacga tacgcaatat ggcctgggcg cgggcgtctg gagccgcaac
     2701 ggtaatctgg cctataagat ggggcgcggc atacaggctg ggcgcgtgtg gaccaactgc
     2761 tatcacgctt acccggcaca tgctgcgttt ggtggctaca aacaatcagg tatcggtcgc
     2821 gaaacccaca agatgatgct ggagcattac cagcaaacca agtgcctgct ggtgagctac
     2881 tcggataaac cgttggggct gttctgatat aagaagctgg tcgcattggg tattcattgc
     2941 ctgatgcgac gcttacgcgt cttatcatgc ctacgggaac ctgaccgtag gccggataag
     3001 gcgtacacgc cgcatccggc ataaacaaag cgcacgttgt tacaatttac gttttaaacc
     3061 tacgtgcgca ataacccaga ctacgccaac agaagtgcgt ttctggggcg tgttttgtca
     3121 ggagtttaat ctcgcgtttc gatggtgagc gggcgaacca ggccgtaaat caccattggt
     3181 gatccgaggc caatcagcaa taaacggtag ataattgaag gtttaatacg cttaatctgt
     3241 actgtggatg ttttactgga gttattcatt agtttggcca atagttccac tatttcaata
     3301 tgataaacct cgtcctgttg aaaaaccaga cagcacattt aagaaagtat ataaaaaaca
     3361 atatgtcgtt atataaataa ggaaattgca ctgcatcatg gcatttcata atatgcgtta
     3421 attgttcttt tttatattct ttgagggaaa tatgttcctg gactattttg cactgggagt
     3481 gcttattttt gtatttctgg tgatcttcta tgggatcatt attttacatg atattcccta
     3541 cctgattgcc aaaaaacgta atcatcctca tgccgacgcc attcatgttg ctggttgggt
     3601 gagtcttttt acgttacatg ttatctggcc gtttttgtgg atttgggcca cgctttaccg
     3661 cccggagcgg ggatggggaa tgcaaagcca tgattcatcc gttatgcaac tgcaacagcg
     3721 cattgccggg ctggagaaac agctcgccga catcaaatcc tcttctgccg agtaatactt
     3781 atggatttac tgattatttt gacctatgtg gcttttgcat gggcaatgtt taagatcttc
     3841 aaaattcccg taaataaatg gaccattccc acagcggccc tgggtggaat atttattgtc
     3901 agcggtctaa ttctgttaat gaactataac catccgtata ccttcaaagc gcaaaaagcg
     3961 gttatttcta ttcctgttgt cccacaggtg acaggcgtgg tgatcgaagt gacggataag
     4021 aaaaatacgc tgattaaaaa aggtgaggtg ctatttcgac tggacccgac gcgttatcag
     4081 gcgcgggtgg atcggctgat ggcggatatc gttaccgcag aacataaaca gcgggcgttg
     4141 ggcgcagagt tagatgagat ggcggcgaat actcagcagg caaaggccac gcgggataaa
     4201 ttcgctaaag agtatcagcg ttacgcacgc ggcagccagg cgaaagtaaa cccgttttca
     4261 gaacgcgata ttgatgtggc gcggcaaaat tatctggcgc aggaagcctc cgtaaagtca
     4321 tcggcggcgg aacaaaaaca gatccagagc cagctggata gcctggtgtt gggtgaacat
     4381 tctcaaatcg ccagcctgaa agcgcagctc gcggaagcaa aatataacct tgagcagacg
     4441 atagtgcgtg cgccgagcga tggttatgtg acccaggtgc tgattcgtcc gggtacctat
     4501 gccgcgtcgc tgccgctacg tccggtgatg gtgtttatac ccgatcagaa acgacaaatc
     4561 gtggcgcagt tccgtcagaa ctccttgctg cgcctggctc ccggcgatga tgcggaagtg
     4621 gtatttaacg ctctgccagg caaagtgttc agcggtaagc tggcagccat tagtccagct
     4681 gttcccggcg gagcttatca gtcgaccggc accttacaga cgttaaacac agcgccgggt
     4741 tcagatggcg ttatcgcgac cattgaactg gatgagcaca ctgatttgag cgctttacca
     4801 gacggtattt acgcccaggt ggcggtctac tctgatcatt tcagccatgt ctcggtgatg
     4861 cgcaaagtgc tgttacgcat gaccagttgg gtgcattacc tttatctcga tcattaatct
     4921 tcgctttccg ccgcttgttc aggccgcagt tgctgcgact gagcaagctg gtggcgaatt
     4981 tcacgcagca tttcatccag cacatactgg acccgccagg gctggtattc ccgtttacgg
     5041 aagatagcca ccagatccag ccaccattca tacgggtgct gaaagcaggg caccagcgtg
     5101 ccgtcttgca gatcgctctg cacacttttg tctggcgcaa acacaatccc caggtgattt
     5161 cgtgccagct ccagcgccga ttgcgtgtta tcgcaaacgt aatttccctt cacgcgataa
     5221 tcccgcacct ctttactccc ggcgacgttg aagcgccaga tattcgcgtc atcaatcatc
     5281 atcgagtcga tcaaaataca ggagtgatgt tcgagttcgt caggacggct aataggatgt
     5341 ttctcaagat agcgctggct ggcataagcg gttacggcat attgggtaat aaaactggca
     5401 accagcgatt catcttttgg tggagcgtag ctgattaaaa catcacaatc atcaggaaat
     5461 tcgacgcctt cataaaaggc attacgctca agattgcagg tttttagcga taaggtgata
     5521 tcaccgatat ctttaatttt atcgattaca tgtttggata aataggtaat tatgccggtt
     5581 ggggcgtaga tggtgacccg accacgtttc tcatgcttat aatctgcaat aaaattatta
     5641 agctgctcgt ttctgtccag catgtcgttg atgtacggta acagcgcggt accaaaaggt
     5701 gtgagcatca gctgccgggt ggttcggtca aagactttta aacccacttt tgattcaaaa
     5761 tcagcaaggt atttgctgac gttggcctgt gcgatgccaa gtacagtcgc ggcatggctg
     5821 atattttcac tggcagcgat taccgagata atttttaact cccggtactt aagttgtaat
     5881 ttt
//
LOCUS       DBANUU010000214         5781 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000214 DBANUU010000000
VERSION     DBANUU010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.217"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            18..758
                     /gene="yiaT"
                     /locus_tag="ACIE68_004436"
     CDS             18..758
                     /gene="yiaT"
                     /locus_tag="ACIE68_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFX5910636.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNKNT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(780..968)
                     /locus_tag="ACIE68_004437"
                     /pseudo
     CDS             complement(780..968)
                     /locus_tag="ACIE68_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            972..1073
                     /locus_tag="ACIE68_004438"
                     /pseudo
     CDS             972..1073
                     /locus_tag="ACIE68_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(1105..1800)
                     /gene="araD"
                     /locus_tag="ACIE68_004439"
     CDS             complement(1105..1800)
                     /gene="araD"
                     /locus_tag="ACIE68_004439"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFX5910637.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDDTRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(1794..2654)
                     /gene="sgbU"
                     /locus_tag="ACIE68_004440"
     CDS             complement(1794..2654)
                     /gene="sgbU"
                     /locus_tag="ACIE68_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFX5910638.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTG
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(2647..3309)
                     /gene="ulaD"
                     /locus_tag="ACIE68_004441"
     CDS             complement(2647..3309)
                     /gene="ulaD"
                     /locus_tag="ACIE68_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFX5910639.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGA
                     RA"
     gene            complement(3306..4802)
                     /gene="lyxK"
                     /locus_tag="ACIE68_004442"
     CDS             complement(3306..4802)
                     /gene="lyxK"
                     /locus_tag="ACIE68_004442"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFX5910640.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTVHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            complement(4806..>5781)
                     /gene="yiaO"
                     /locus_tag="ACIE68_004443"
     CDS             complement(4806..>5781)
                     /gene="yiaO"
                     /locus_tag="ACIE68_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFX5910641.1"
                     /translation="SVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAAKKFN
                     DLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMNLLDV
                     PFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTPADLK
                     GLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAKFFEV
                     QKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGNYQRKLVAEDQQKIID
                     GMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
BASE COUNT         1239 a         1591 c         1591 g         1360 t
ORIGIN      
        1 aaaatggaat accggacatg ttaattaacc gcaatattgt ggcgttattt gcgttgcctt
       61 ttatggcaag cgcaactgct tctgaattat ccattggtgc tggtgcggct tataatgaat
      121 cgccttatcg cggttataat aaaaatacaa aggcaattcc gctgattagt tatgaaggtg
      181 attcttttta tgttcgtcag accacgttag gttttattct gtcgcaaagt gaaaaaaatg
      241 aacttagcct gaccgcatcc tggatgccgc tggaatttga ccctgccgat aatgacgatt
      301 atgccatgca acagcttgat aagcgtgata gtacggctat ggcgggggtt gcctggtatc
      361 accacgagcg ttggggaacc gtgaaagcct ctgcggctgc ggacgttctg gataacagca
      421 acggctgggt gggggagcta tcggtattcc acaaaatgca gataggtcgc ctgtcgctga
      481 cacctgcgct gggcgttctc tattatgacg agaatttcag tgactattac tatggcattt
      541 cagagagtga gtcccgtcgt agcggtctgg caagttattc cgcgcaggat gcctgggtgc
      601 cctatgtcag cctgacggca aaatacccga taggagagca cgtcgtattg atggcgagcg
      661 caggatacag cgagctgccg gaagagatta ccgacagccc gatgattgat cgtaatgaga
      721 gttttacctt tgtcaccggg gtgagctggc gtttttaatt caccggtgga tgtcggtgcg
      781 gctggtgctc cgtcgtaatg ccacaaaggt tgttgctctg gattgctctg ttcataccgg
      841 gaacctggcg tctgaatcag gatcttatgg ctgaacaatc ccttgccttc aaatgtcgta
      901 aatggcgcat cggcgggcag caacaggtta tgcagtgact cgacgttgtc ggcctgctcc
      961 atgcaatacg gtttaatgac gttggctatt tccggcagat ttttcagaaa catactggtt
     1021 taacgccagc agcgtggaag cggcgttaca gcaaggaaca tatcaattcg tagtgccggg
     1081 gcgatgaagc cccggcgtga gggattactg cccgtaatag gcattggccc catgcttacg
     1141 caggtagtgc ttatccagca gttcgttttg catcgcaggg agctgcggcg caagctggcg
     1201 cgagaataga cccatatagg cgcattcttc gagtactacg gcgttatgca cggcatcggc
     1261 ggcgttttta ccccatgcga acgggccgtg agaatgcacc agcaccgccg ggatttgtgc
     1321 cggactcctg ccacgttctt cgaaggtttc aatgatcact tcgccggtct gatattcata
     1381 ttcgccgtta atctcctctg cggtcatctg tcgcgtgcag gggatagcac cgtaaaaata
     1441 atcggcgtgg gtggtgcccc aggcggggag atccagcccg gcctgtgacc agatggtggc
     1501 gtggcgcgag tgggtatgca caatgccacc aatttcggca tagcgacggt aaagtgccag
     1561 atgcgttggc gtatcggacg agggcttttt gctaccttcc accaccttgc cgctggctat
     1621 ctcaaccacc accatatcgt cggcggtcat cacgtcgtac tcgacgccgg aaggtttgat
     1681 taccatccat tgccgcgtgt cgtctaccgc gctgacattg ccccaggtga acgtcaccag
     1741 atggtgagcg ggaagcgcca gattcgccgc cagcacgtcg gctttcagtt gctctaacat
     1801 ataaatccag cctcctgcat acgcgcttca atccagcgcc gcgcctgaat gatctccagc
     1861 accggctctt tggctttttc ggtccacatc tcaatcagga aagaaccgcg atagttcagt
     1921 tcatgcagcg ttttgaagat gccaacgaaa tcgacgcagc cttcgccaaa cggcacatca
     1981 cggaactgtc cggggctttg cccggtgact ggctgggtgt ctttcaggtg gatcgcggcg
     2041 atacggtcaa tgcccagttt cagttcggcg ggaacatcat tgccccaggc gctgaggttg
     2101 ccgacgtccg ggtagacggt gaaccacggc gaggcgagca tctcgtccca ttttttccat
     2161 ttgctgatgg agttcataaa cgcggtatcc ataatctcca ccgccagcat tacttgtgat
     2221 gccgccgcct gttcgactgc ccacgccagc ccttcagcaa aacgttgccg ggtgccttcg
     2281 tcgtggtctt cgtaatagac gtcataacct gccagctgga tggtgcgaat gccgagatcg
     2341 cgcgccaggc gaatcgcttt actcatgatt tcccgcgccc gttcgcgcac tgcctcgtca
     2401 cggctaccaa agggaaagcg acgatgtgcg gacaggcaca tcgacgggat cccaacacct
     2461 gtttcgatca tcgcggcaac cagcgaagtc ctttgtgcgg cgctccagtc aagacgtgag
     2521 agccgttcgt cggtttcatc caccgacatt tcgacaaaat caaaaccgca gctttttgcc
     2581 agcaccagcc gctccggcca ggagagatct ttcgccagcg ctttttcata aatccctaac
     2641 ggatgattac gcacgcgcac ctccccagat agcgtcgatt tgcgcatgga aatcgccagc
     2701 cacttgcgcc ggatttgccg cgcctgccag tgcccgcccg gcaataaacg ctttcacgcg
     2761 gatatcttta aacagcggca ggtcagcagg ggtaatcccg ccagtaatgg aaagctcaag
     2821 gccgatatct gaaagcgcct tcatgcgtgc cagatcggct tcgccccact gttgcccgct
     2881 ggcctgtgca tcacgaccgc gatgataaat ggcctgccgc acgccaatac gatgccagtc
     2941 gcgggcgtcg tccagcgtcc agttaccgaa cagctctatc tgaatttcac ccccgcagcg
     3001 ttgtgccatt gcgtggcctt tttctaccgt cgcgagcggc gcggcgcaga tgatggtcat
     3061 ccagttagcg cctgcgccaa acgcctgttg cgcgagcgtt tcaccagcgt cggcgacctt
     3121 ccagtcagca acgatgattt tgtccgggca ctgttcgcgc aaggctttca ccgcgccaag
     3181 cccttcgttt aaacagagaa tggtgcccgc ttcgacaata tcgacgctgt cttttaactg
     3241 cgtcacgtcg cgctgtgcgg cttcaagtga tgagtggtcg agtgccagtt gcagaagtgg
     3301 tcggctcata atatgtgctc cttgatgcgg gcgtgaaagc cctgaagtgc ggcaatgaga
     3361 tgctgatagc gttggtattt ttgttggtaa agctgatgga cagtcatatc cggcagcagg
     3421 gtgcgcaccg ggtgctgtaa atcacgctgg gcttcgctga aatcgcgata aaccccggtg
     3481 ccgacgcggg cggcaagtgc tgcaccaaag cagccggttt cttccacctg cggcagctca
     3541 atacgcagac cgctgacgtc cgccagcatt tgcatccaga catcggaatg cgccgggccg
     3601 ccagtgacgc gcagggtgtg cacatcagta aaacgttcgc gcatccggtt gagatgggtc
     3661 atatggctga acaccacccc ttcataaatg gcctgcaaca ggtgtgcgcg ggtgtgaatg
     3721 gcctgcatcc cgtagaaacc gctggtcatc tcgagtccgg cgttgctgcc gtacaggaac
     3781 ggcaggaaaa agagatcgcc accggccttc ggcaagctgg caacggcctg gttgatctca
     3841 tcaaacgaga tttctcccca ctgtgcggta aaccattcga ggttgccgga agaggtgggg
     3901 ctggcttcgt gaacgataaa ttcaccatcg ttaacgtagc gaccatagac atacggatgc
     3961 gcttcaccgt cacgtaaacc gcgggttatg ccgctggtca ccgcccaggt gcccatcacc
     4021 gcattaaggg taaattcgtc ttcgatcccg gcgcagagtg cggtggaaac cacatcaaac
     4081 aggccgccga caacgggcgt acccgctttc agaccggtca gtacggctgt ctgagcggtg
     4141 atctccccgc agatttcggc agatccgaca acaggcggca gggcgtgatt gatttcagca
     4201 atccccagcc agtcggtgag gcacgggtca tattccccaa gactcatgtt gtagaggttg
     4261 gactcggaaa tattgctctc ttcacagcct ttgacgccgg ttaaacacca gcgcaggtag
     4321 tcgtgcgtca tcatcacgca gccaatttgc gcgtagcgtt ccggttcgtg ctctttcagc
     4381 cagcgtaaca gcgacaccgg atgcccggtc cacaaggttt gtcgggtcaa cgggtagagt
     4441 ttttccggga tgccatcttc ctgccagcga cgaacgattt ccatcgcccg gcgatccgag
     4501 gacaaaatgg cgttaccgag cggcttgttg tttttatcca gcaaaaacaa gccttttcct
     4561 tgtgcggaga taccgatacc gacaatttgt tccccgctaa cgccggaatg ggtaagcagt
     4621 tcgcgaatga cagccatgca gcattgccac agttctgcca tatcgcgctc tgcccaacct
     4681 ggctgcgggc ttaatgcgca cagcggcagg cgctgcacgc ctgcctcccg gccttcgcgg
     4741 tcatacagcc cggctttcag ccagctaccg ccacaatcta accccagcca gtattgcgtc
     4801 atggtttatt gcacctcatc cacggctttc agtagatcag caccctgcgg aacatctttt
     4861 acaaacatgt cgcggacctg agtacccagt gcgtcgctaa aggctttgcg gtcgagatcg
     4921 gtgatgactt ccacgcccgc ttctttcatg ccgtcgatga ttttttgctg atcttcggca
     4981 accagtttgc gctggtagtt acccgcttcc tgcgcagatg aaattagcgc ttgctggaac
     5041 tccggggtta agccatcaaa cttcgctttg ttgatcacca ccagaagcgg ggaataggcg
     5101 tggtgcgtca gagaaaggta cttctgcact tcgaaaaatt ttgctgacca gacgacgttg
     5161 atcgggtgtt cctgagcgtc gatagtgcgg gtttccagcc cggtatagac ttcggcaaac
     5221 ggcatcggga tcgggttagc gccaaagact ttgaatgcgg cgatattcat cgggctattg
     5281 ttggtgcgaa ttttcagccc tttcaggtcg gcgggggttt taaccggtgc gcgcgagttg
     5341 gtgacatcgc gccagccgtt ttcccagtag gccagtactt ttagtccttt accttcaagt
     5401 gaggctttca gatcatcacc gactttgccg tcgagcgttt tatgcgcgtg agcggtatcg
     5461 cggaacagga aagggacatc aagcaagttc atcactggtg ataacccggc aaagttattc
     5521 gagccggaca tttccatatc gatggtgccg ccacgtacgc cgctgatcat cgcctgcgcg
     5581 ttaccgaggg tgctatccgg aaacaatttt aatttcagct cgcctttggt tctctcctgc
     5641 aataaatcat tgaatttttt cgccgcaata tgttgcgagt cggtttgtga ggtttcataa
     5701 ccgaaacgta aagattgcgc cgccagagaa gatgtgctga atgcagccag gccagcaatg
     5761 aataatgcgt aggttacaga g
//
LOCUS       DBANUU010000215         5774 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000215 DBANUU010000000
VERSION     DBANUU010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.218"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(13..156)
                     /gene="ypaB"
                     /locus_tag="ACIE68_004444"
     CDS             complement(13..156)
                     /gene="ypaB"
                     /locus_tag="ACIE68_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFX5910642.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            284..2569
                     /gene="nrdA"
                     /locus_tag="ACIE68_004445"
     CDS             284..2569
                     /gene="nrdA"
                     /locus_tag="ACIE68_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFX5910643.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2804..3934
                     /gene="nrdB"
                     /locus_tag="ACIE68_004446"
     CDS             2804..3934
                     /gene="nrdB"
                     /locus_tag="ACIE68_004446"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFX5910644.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            3934..4188
                     /gene="yfaE"
                     /locus_tag="ACIE68_004447"
     CDS             3934..4188
                     /gene="yfaE"
                     /locus_tag="ACIE68_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFX5910645.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4242..4892)
                     /gene="inaA"
                     /locus_tag="ACIE68_004448"
     CDS             complement(4242..4892)
                     /gene="inaA"
                     /locus_tag="ACIE68_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFX5910646.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(4972..5076)
                     /locus_tag="ACIE68_004449"
                     /pseudo
     CDS             complement(4972..5076)
                     /locus_tag="ACIE68_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5071..5313
                     /locus_tag="ACIE68_004450"
                     /pseudo
     CDS             5071..5313
                     /locus_tag="ACIE68_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1458 a         1499 c         1396 g         1421 t
ORIGIN      
        1 ctattatgcc gttcaagaaa tcgccgaaca gttattttta acaaattttt ctcttcccat
       61 tgactttccc ggacaccttg tctgacctaa ggtgcgcgaa agccactttt tccttcctga
      121 gttatccaca aagttatgca cttgcaagag ggtcattttc acactatctt gcagtgaatc
      181 ccaaacatac cccctatata tagtgttcta agcagcttcc cgtactacag gtagtctgca
      241 tgaaactatt gcggaaagaa ttccaaaaac aggtacgaca tacatgaatc agaatctgct
      301 ggtgacaaag cgcgacggta gcacagagcg catcaatctc gacaaaatcc atcgcgttct
      361 ggattgggcg gcagaaggac tgcataacgt ttcgatttcc caggtcgaac tgcgctccca
      421 cattcagttt tatgacggta tcaagacctc tgacatccac gaaaccatta tcaaggctgc
      481 cgcagacctg atctcccgtg atgcgccgga ttatcagtat ctcgccgcgc gcctggcaat
      541 cttccacctg cgtaaaaaag cctacggcca gtttgagccg cctgcgctgt acgaccatgt
      601 ggtgaaaatg gtcgagatgg gcaaatacga taatcatctg ctggaagact acacggaaga
      661 agagttcaag cagatggaca cgtttatcga tcacgaccgt gatatgacct tctcttatgc
      721 tgccgttaag cagctggaag gcaaatatct ggtacaaaac cgcgtaaccg gcgaaatcta
      781 tgagagcgcc cagttccttt atattcttgt tgccgcgtgc ctgttctcga actacccgcg
      841 tgaaacgcgc ctgcaatacg tgaagcgttt ttacgacgcg gtttccacat ttaaaatttc
      901 gctgccgacg ccaatcatgt ccggcgtgcg taccccgact cgtcagttca gctcctgcgt
      961 actgatcgag tgcggtgaca gcctggattc catcaacgcc acctccagcg cgattgtgaa
     1021 atatgtttcc cagcgtgccg ggatcggcat caacgccgga cgtattcgtg cgctgggcag
     1081 cccgattcgc ggtggcgaag cgttccatac cggctgtatc ccgttctaca aacatttcca
     1141 gacagcggtg aaatcctgct ctcagggcgg tgtgcgcggc ggtgcggcaa cgctgttcta
     1201 cccgatgtgg catctggaag tggaaagcct gctggtgttg aaaaacaacc gtggggttga
     1261 aggcaaccgc gtgcgtcata tggactacgg ggtacaaatc aacaaactga tgtatacccg
     1321 tctgctgaaa ggtgaagata tcaccctgtt cagcccgtcc gacgtaccgg ggctgtacga
     1381 cgcgttcttc gccgatcagg aagagtttga acgtctgtat accaaatatg agaaagacga
     1441 cagcatccgc aagcagcgtg tgaaagccgt tgagctgttc tcgctgatga tgcaggaacg
     1501 tgcgtctacc ggtcgtatct atattcagaa cgttgaccac tgtaataccc atagcccgtt
     1561 tgatccggcc atcgcgccag ttcgccagtc taacctgtgc ctggaaattg ccctgccgac
     1621 caaaccgctg aacgacgtca acgacgagaa cggtgaaatc gcgctgtgta cgctgtctgc
     1681 tttcaacctg ggcgcaatta atagcctgga tgaactggaa gagctggcaa ttctggcggt
     1741 tcgtgcactt gacgcgctgc tggattatca ggattacccg atcccggccg ccaaacgtgg
     1801 agcgatgggt cgtcgtacgc tgggtattgg tgtgatcaac ttcgcttact acctggcgaa
     1861 gcacggtaaa cgctactccg acggcagcgc caacaacctg acgcataaaa ccttcgaagc
     1921 cattcagtat tacctgctga aagcctctaa tgagctggcg aaagagcaag gcgcgtgccc
     1981 gtggtttaac gaaaccactt acgcgaaagg gatcctgccg atcgatacct ataagaaaga
     2041 tctggatacc atcgctaatg agccgctgca ttacgactgg gaagctctgc gtgagtcaat
     2101 caaaacgcac ggcctgcgta actccacgct ttctgctctg atgccgtccg agacttcttc
     2161 gcagatctct aacgccacta acggtattga accgccgcgc ggctacgtca gcatcaaagc
     2221 gtcgaaagac ggtattctgc gccaggtggt gccggactac gagcatctgc acgacgccta
     2281 tgagctgctg tgggaaatgc cgggtaacga tggctacctg caactggtgg gtatcatgca
     2341 gaaatttatc gatcagtcga tctctgctaa caccaactac gatccgtcac gcttcccgtc
     2401 aggaaaagtg ccgatgcagc agttgctgaa agacctgctc accgcctaca aattcggggt
     2461 caaaacgctg tattatcaga acacccgtga cggcgctgaa gacgcgcagg acgatctggt
     2521 gccgtcaatc caggacgatg gctgcgaaag cggcgcatgt aagatctgat attgagatgc
     2581 cggatgcggc gtaaacgcct tatccggcct acggctcgga tttgtaggcc tgataagacg
     2641 cgccagcgtc gcatcaggct ccgggtgccg gatgcagcgt gaacgcctta tccggcctac
     2701 ggctcggatt tgtaggcctg ataagacgcg tcagcgtcgc atcaggcaca ggatgcggcg
     2761 taaaatgcct tatccggcat taaactccca acaggacaca ctcatggcat ataccacctt
     2821 ttcacagacg aaaaatgatc agctcaaaga accgatgttc tttggtcagc cggtcaacgt
     2881 ggctcgctac gatcagcaaa aatatgacat cttcgaaaag ctgatcgaaa agcagctctc
     2941 tttcttctgg cgtccggaag aagttgacgt ttctcgcgac cgtatcgatt accaggcact
     3001 gcctgaacac gaaaaacaca tctttatcag caacctgaaa tatcagacac tgctggattc
     3061 cattcagggc cgtagcccga acgtggcact gctgccgctg atctcgatcc cggaactgga
     3121 gacctgggtc gaaacctggg cgttttcaga aacgattcat tcccgttcct atactcatat
     3181 cattcgtaat atcgttaacg atccgtctgt tgtgtttgac gatatcgtca ccaacgagca
     3241 gatccagaaa cgtgcggaag ggatctccag ctattacgat gagctgatcg aaatgaccag
     3301 ctactggcat ctgctgggcg aaggtaccca caccgttaac ggtaaaactg tgaccgttag
     3361 cctgcgcgag ctgaagaaaa aactgtatct ctgcctgatg agcgttaacg cgctggaagc
     3421 gattcgtttc tacgtcagct ttgcttgttc cttcgcattt gcagaacgcg aattgatgga
     3481 aggcaacgcc aaaattattc gcctgattgc ccgcgacgaa gccctgcacc tgaccggcac
     3541 ccagcatatg ctgaatctgc tgcgcagcgg cgcggacgat cctgagatgg cggaaattgc
     3601 cgaagagtgt aagcaggagt gctatgacct gtttgttcag gcagctcaac aggagaaaga
     3661 ctgggcggat tatctgttcc gcgacggttc gatgattggt ctgaataaag acattctctg
     3721 ccagtacgtt gaatacatca ccaatatccg tatgcaggca gtcggtttgg atctgccgtt
     3781 ccagacgcgc tccaacccga tcccatggat caacacctgg ctggtgtctg ataacgtgca
     3841 ggttgctccg caggaagtgg aagtcagttc ttatctggtc gggcagattg acgcggaagt
     3901 ggacaccgac gatttgagta acttccaact ctgatggccc gcgttaccct gcgcatcact
     3961 ggcacacaac tgctgtgcca ggatgaacac ccttcccttc tggcggcgct ggaatcccac
     4021 aatgtggcgg ttgagtatca gtgtcgcgaa ggttactgcg gctcctgtcg cacacgcctg
     4081 gttgcaggtc aagttgactg gattgccgaa ccgttagcct ttattcagcc gggggaaatt
     4141 ttgccctgtt gttgccgggc aaaaggcgat attgaaatcg agatgtgaat tggttgtagt
     4201 gccagataca acgcttatgc gtcttatctg gcctacaacg attacatggc gtagtaatac
     4261 gctttcacct gctcccagtc cgctttgggg attggctcca gatatttttc cagctggcgg
     4321 aagtcatgat taatcgcttt atcgcgacgc aagcggcgac gacttttctc caggtcaaga
     4381 aaaccagctt ctgccttacc ttctgttttc acatagatat ggcgaacata acaacagcca
     4441 tgctgacgat taatgctatg cattttctta aacgccagcg ccactgcttt caacatggct
     4501 tgccgtactt cgtcagaata aggcgatact gcatgctggg cataccagtc agcaatgctg
     4561 atgaaccccg ccatatcttc agtcaccagc aacgctcgcc attcaccctc aattttcacc
     4621 gcttcaccaa aaacgatctt cggcacgatg acaccggccc gttccagttc tttaattacc
     4681 gcaacctcac ggacaatcgt tggtcggccg aacggataac gtacggaatg aaacagatga
     4741 tgcgtcatgc gctttacata cagctttttg ccgttgcgct cgacgcattg caccccgctc
     4801 ataccattac ggcgatagtt aggctcttca acccagtcgc cctctgttgc ccaccagtga
     4861 ttaaattcgt cgtactttgc tgaaactgcc ataccctatc gcctgtcatt tttattaacg
     4921 acaatgacta taggtggtta cctgaggaaa atcttaatga aacgtgtcgt attaatgaat
     4981 ttcttttgcc actactagcg tgacaccgct tttacccttc atttgcagcg catagattcc
     5041 accagccact accactaata gtgcgcccag cagataaaat gtgaatttaa tcaggcggat
     5101 acagcgatga attttattcg acagggatta ggcatcgcct tacaaccaga gttaacgctg
     5161 aaaagcattg caggtgaatt gtgttccgtt cctctcgaat cgaccttcta tcgacagatt
     5221 tcgttgttgg ctaaagaaaa gccggtagaa ggcagtccac tgtttttact acaaacgtgt
     5281 acggaacaac tcgtggtgag tggaaaaatt tgatgtgaga catgagattg atgacaaatg
     5341 taagattgcc tgattcgcca cacttagttg gcctgtagtc tttaccaggc gtaataattc
     5401 gttttcacct gattttggtc cactttgtga attagcgata tatatttatc cagtttaggg
     5461 aagtgacaat gactccaggt agaaaccggt atgaagcgcc cgactcactg tcaaaaccga
     5521 ttgttgagag taatgacact ccggcgaaca gtacacagtt tactctggag ccattctcaa
     5581 tcttcaagtg ggatacctaa taacttgtca gggaagttat caatatctgt ttgttactta
     5641 ttaccgtgtt tattaacaat aatgatactt tctaacatgc gtaaatgtct tcgtaataaa
     5701 atgagctaaa tttgatagtg aatatattct gcaaagatta aattgtatct tgttaactca
     5761 caatacaagg aatc
//
LOCUS       DBANUU010000216         5765 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000216 DBANUU010000000
VERSION     DBANUU010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.219"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..607
                     /gene="pdeC"
                     /locus_tag="ACIE68_004451"
     CDS             <1..607
                     /gene="pdeC"
                     /locus_tag="ACIE68_004451"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFX5910647.1"
                     /translation="EGMIEQITDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSR
                     LIARINQKTEQYAVRPQQIKFEVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYS
                     NLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQV
                     NWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLPARELTRGQ"
     gene            complement(610..933)
                     /gene="soxS"
                     /locus_tag="ACIE68_004452"
     CDS             complement(610..933)
                     /gene="soxS"
                     /locus_tag="ACIE68_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFX5910648.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1019..1483
                     /gene="soxR"
                     /locus_tag="ACIE68_004453"
     CDS             1019..1483
                     /gene="soxR"
                     /locus_tag="ACIE68_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFX5910649.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2029..3378
                     /gene="ghxP"
                     /locus_tag="ACIE68_004454"
                     /gene_synonym="yjcD"
     CDS             2029..3378
                     /gene="ghxP"
                     /locus_tag="ACIE68_004454"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFX5910650.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            3529..5178
                     /locus_tag="ACIE68_004455"
     CDS             3529..5178
                     /locus_tag="ACIE68_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFX5910651.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(5332..>5765)
                     /locus_tag="ACIE68_004456"
     CDS             complement(5332..>5765)
                     /locus_tag="ACIE68_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFX5910652.1"
                     /translation="DLSRVNLNKAILQSINLIDSKLFCTWLTNTFLEMVICTGSNMAN
                     VNFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDT
                     LVTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
BASE COUNT         1259 a         1356 c         1584 g         1566 t
ORIGIN      
        1 ggaggggatg atagaacaga taactgatta tgttattgat aatgtcttcc gcgatctggg
       61 cgattacctg gcaacacatg cagatcgcta tgtttctatt aacctgtcgg cctccgattt
      121 tcatacgtca cggttgatag cgcgaatcaa tcagaaaaca gagcaatacg cggtgcgtcc
      181 gcagcaaatt aaatttgaag tgactgaaca tgcatttctt gatgttgaca aaatgacgcc
      241 gattattctg gctttccgcc aggcaggtta cgaagtggca attgatgatt ttggtattgg
      301 ctactctaac ttgcataacc ttaaatcatt gaatgtcgat attttgaaaa tcgacaaatc
      361 gtttgttgaa acgctgacca cccacaaaac cagtcatttg attgcggaac acatcatcga
      421 gctggcgcac agcctggggt taaaaacgat cgctgaaggc gtcgaaactg aggagcaggt
      481 taactggctg cgcaaacgcg gcgtgcgcta ttgccaggga tggttctttg cgaaggcgat
      541 gccgccgcag gtgtttatgc aatggatgga gcaattaccc gcgcgggagt taacgcgcgg
      601 gcaataaaat tacaggcggt ggcgataatc gctgggagtg cgatcaaact gccgacggaa
      661 aacgcgggag aaggtctgct gcgagacata acccaggtcc attgcgatat caaaaatcgg
      721 acgctcggtg gtgcgcaact caacggcggc cagtaacagg cggcgttggc gaatgtaatc
      781 gccaagcgtc tgatgcgtca ccgtgcggaa cattcgttgc aagtaccact ttgaatagcc
      841 tgattttttt gcgactacat caatgttaag cggctggtca atatgctcgt caatccatgc
      901 gataagatcc tgaataattt tctgatggga cataaatctg cctcttttca gtgttcagtt
      961 cgttaattca tctgttgggg agtataattc ctcaagttaa cttgaggtaa agcgatttat
     1021 ggaaaagaaa ttaccccgca ttaaagcgct gctaaccccc ggcgaagtgg cgaaacgcag
     1081 cggtgtggcg gtatcggcgc tgcatttcta tgaaagtaaa gggttgatta ccagtatccg
     1141 taacagcggc aatcagcggc gatataaacg tgatgtgttg cgatatgttg caattatcaa
     1201 aattgctcag cgtattggca ttccgctggc gaccattggt gaagcgtttg gcgtgttgcc
     1261 cgaagggcat acgttaagtg cgaaagagtg gaaacagctt tcgtcccaat ggcgagaaga
     1321 gttggatcgg cgcattcata ccttagtggc gctgcgtgac gaactggacg gatgtattgg
     1381 ttgtggctgc ctttcgcgca gtgattgccc gttgcgtaac ccgggcgacc gcttaggaga
     1441 agaaggtacc ggcgcacgct tgctggaaga tgaacaaaac taaagcgcca caagggcgct
     1501 ttagtttgtt ttccggtctt tgtctttctc tctatcccgc tggtacacag gagggtttcc
     1561 cccgacgtca acacacctca ttcgagcacg tggtggaggt tccggttggt gttgatgctt
     1621 taattgtatg tcaccgacgt ttcttcgcca gtgtaaaagt atacttttta accgcaatat
     1681 ttttgtcatc tcagacgatt ttttatcgca atcctgaacg gtatacggct cgataacgct
     1741 gcaatcttgc gcaccgacga taacgtttgc gcatcaattg cctggttttt catcgtcaag
     1801 acaataaaag agaaaaaagc agcaaacttc ggttgaaaaa gccgctatga tcgccggata
     1861 atcgtttgct ttttttacca cccgttttgt atgcgcggag ctaaacgttt gcttttttgc
     1921 gacgcagcaa attgtcgcaa acctggagca ggaagataac gtttcgctgg caggggattg
     1981 tccgccacgc atcttgacga aaattaaact ctcaggggat gttttcttat gtctacgcca
     2041 tcagcgcgta ccggcggttc actcgacgcc tggtttaaaa tttcacaacg tggaagcact
     2101 gtccgtcagg aagtggttgc cgggttaaca acgtttctgg cgatggtcta ctcggtcatc
     2161 gtcgttccag gtatgttggg taaagcaggc ttcccgcctg cggcagtttt cgttgcaacc
     2221 tgtctggttg ccggactcgg ttctatcgtg atgggtctgt gggctaatct gccgttggcg
     2281 attggttgcg ccatctccct gacggcattt accgcattca gcctggtgct ggggcaacat
     2341 attagcgtac ctgtcgcgct gggtgccgtg ttcctgatgg gtgtgctgtt tacggtaatt
     2401 tctgccacgg gtatccgtag ctggattttg cgcaacttgc cgcacggtgt ggcgcacggc
     2461 acggggattg gtatcggtct gttcctgctg ctcattgccg ctaacggtgt cggtctggtg
     2521 attaaaaacc cgcttgatgg tctgcccgtt gcgctgggtg atttcgcgac cttcccggtg
     2581 attatgtcac tggtaggtct ggcggtgatc atcggcctgg aaaaactgaa agtccctggt
     2641 ggcattctgc tgaccattat cggtatctca attgtcggtt tgatcttcga tcctaacgtc
     2701 catttctccg gcgttttcgc catgccttca ttgagcgatg aaaacggcaa ctcactgatt
     2761 ggcagcctgg acattatggg cgcgctgaat cctgtagtcc tgccaagcgt tctggcgctg
     2821 gtaatgacgg cagtatttga tgccaccgga actatccgtg ccgtcgccgg ccaggcgaac
     2881 ctgctggata aagatgggca aattatcgac ggtgggaaag cactgaccac tgactccatg
     2941 agcagcgttt tctctggcct ggtgggtgca gctccggcag cggtatacat cgagtctgcg
     3001 gcgggtacgg cggcgggcgg taaaaccggt ttgacggcta tcaccgttgg cgtgctgttc
     3061 ctgctgattc tgttcctctc tccgctctct tacctcgttc cggggtatgc aacggctccg
     3121 gcgctgatgt acgttggcct gctgatgctg agcaacgtgg cgaaaatcga ctttgctgat
     3181 tttgttgatg cgatggcggg tctggttacg gcggtattca tcgtgctgac ctgtaacatc
     3241 gtaacaggca tcatgatcgg cttcgcgact ctggtgattg gtcgtctggt ttccggcgaa
     3301 tggcgcaagt tgaacatcgg tacggtcgtt atcgccgtgg cgctggtgac cttctatgcg
     3361 ggtggctggg ctatctaatc tttcatcgga ttctgaaaac gggtggcaat ggctgcccgt
     3421 ttttattttc tccgcacatt gtgtgagctt ttgcgatatt ctgaaaaaat gagaattcag
     3481 gcataacgtc tttctcagat cataaaaaac atcgcaaaca gggaacgcat ggaaatcttc
     3541 ttcaccatac tgataatgac cctcgtggtc tcgctgtccg gggtggtcac tcgtgtcatg
     3601 ccctttcaga tcccgcttcc gcttatgcaa atcgccatcg gtgcgctact ggcgtggccg
     3661 acgtttggtt tgcatgtgga gtttgatcct gaactctttt tagtcttgtt tatcccgccg
     3721 ttgctgttcg ctgatggctg gaaaacgccg acccgtgaat ttctcgaaca tggtcgagag
     3781 attttcggcc tcgcgctggc gctggtggtg gtcaccgtgg tcggcattgg cttccttatt
     3841 tactgggtgg tgccgggcat tccgctgatc cccgcctttg cgctggcggc ggtactttct
     3901 ccgaccgatg ctgtggcgct ctccgggatt gttggcgaag ggcgcatccc gaaaaaaatc
     3961 atgggcattt tgcagggcga agcgttgatg aatgacgctt ccggcctggt gtcgttgaag
     4021 tttgccgtgg cagtggcgat ggggacgatg atcttcaccg tcggcggtgc gacggtcgaa
     4081 tttatgaaag tagccattgg cggtattctt gccggttttg tggtgagctg gctgtatggt
     4141 cgctcgctgc gattcctcag ccgctggggc ggtgatgaac ccgcgacgca gattgttctg
     4201 ctgttcttgc tgccattcgc ttcttatctg attgccgaac atattggcgt ttccggcatc
     4261 ctcgctgcgg ttgccgccgg gatgaccatc acccgctccg gtgtgatgcg ccgtgcgccg
     4321 ctggcaatgc gcctgcgtgc aaacagcacc tgggcgatgc tggaatttgt ctttaacggc
     4381 atggtgttcc tgctgttagg tctgcagctg ccgggtattc tggagacgtc gctgatggcg
     4441 gcagaaatcg accctaacgt cgaaatctgg atgctgttta ccgatattat tctgatatat
     4501 gcggcgctga tgctggtccg tttcggctgg ctgtggacga tgaaaaagtt cagcaaccgc
     4561 ttcctgaaga agaagccgat ggagtttggt tcgtggacca cacgagaaat cctgatcgcg
     4621 tctttcgccg gggtgcgtgg ggcgatcact ctggccggtg tgctctctat cccgctgctc
     4681 ttgccggatg gtaacgtctt cccggcgcgc tatgagctgg tgttcctggc ggctggcgtc
     4741 attctcttct cgctgtttgt cggcgtggtg atgttgccta ttctgctaca acacattgaa
     4801 gtcgcggacc attcgcaaca attgaaagaa gaacgtattg cgcgagcggc aacggcagaa
     4861 gtggcgattg tggcgatcca gaaaatggag gagcgtctgg cggcggatac cgaagagaat
     4921 atcgataacc agctgcttac ggaggtcagt tctcgcgtca ttggtaacct gcgtcgtcgc
     4981 gccgatggac gtaacgacgt tgaaagttcc gtgcaggaag agaaccttga gcgtcgcttc
     5041 cgtctggcgg cattgcgttc tgaacgtgct gaactgtacc acctgcgcgc cacgcgggag
     5101 atcagcaacg aaacgctgca aaaattactg cacgatctcg atttgcttga agcgttgcta
     5161 attgaggaaa atcagtaagc cgaaagcgac gtaaccaggc ccgaggtaat ctgaaaaagg
     5221 tgcaatcttt tgcaccttta acaggttagc tcgggctaac attcatgatt ctaaaacaaa
     5281 taagttatct ctcttgtaaa gtcaattcat tggctgttct tcgacttaac ctcaatctgc
     5341 gtgttgcata atatctgcag taatcttcat agcgactgca ttaatattat cctcagtttg
     5401 tgacatgcca gtatgagagg tacaatcttc ttcaaagaat ttctgtagtg ttaccagggt
     5461 atcactatca atggggatat tttcctcccc acggagaata gaaatgccca ttccctggac
     5521 gctagcctca tcaaaattaa cccgatagag acgggtatta aacatacagg cttttgttaa
     5581 aatagaacag ttgaaatggc agttgcttaa attggcatta ttaaaattaa cattagccat
     5641 attagagccg gtacatataa ccatttcgag gaatgtattt gttagccacg tacaaaagag
     5701 tttgctatca attaagttta tactctggag gattgctttg ttcagattga cgcgagacag
     5761 atcag
//
LOCUS       DBANUU010000217         5765 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000217 DBANUU010000000
VERSION     DBANUU010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.220"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(326..1285)
                     /gene="nrdF"
                     /locus_tag="ACIE68_004457"
     CDS             complement(326..1285)
                     /gene="nrdF"
                     /locus_tag="ACIE68_004457"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFX5910653.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1295..3439)
                     /gene="nrdE"
                     /locus_tag="ACIE68_004458"
     CDS             complement(1295..3439)
                     /gene="nrdE"
                     /locus_tag="ACIE68_004458"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFX5910654.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFHTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYKIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3412..3822)
                     /gene="nrdI"
                     /locus_tag="ACIE68_004459"
     CDS             complement(3412..3822)
                     /gene="nrdI"
                     /locus_tag="ACIE68_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFX5910655.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIAQKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3819..4064)
                     /gene="nrdH"
                     /locus_tag="ACIE68_004460"
     CDS             complement(3819..4064)
                     /gene="nrdH"
                     /locus_tag="ACIE68_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFX5910656.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4312..4641)
                     /locus_tag="ACIE68_004461"
     CDS             complement(4312..4641)
                     /locus_tag="ACIE68_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFX5910657.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4793..5137
                     /locus_tag="ACIE68_004462"
     CDS             4793..5137
                     /locus_tag="ACIE68_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFX5910658.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5174..5623)
                     /gene="alaE"
                     /locus_tag="ACIE68_004463"
     CDS             complement(5174..5623)
                     /gene="alaE"
                     /locus_tag="ACIE68_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFX5910659.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         1478 a         1421 c         1429 g         1437 t
ORIGIN      
        1 gtcgatatat tacccaatgg aaatagtcac ttttttctac cctaacatac tgagaatctg
       61 aggcaacccc tgagggcaaa tgtggcatga atattgatgc gagtaaatcg ccggaatttc
      121 ccgtgatata agggctgaga gcaaatcgag aaaagttgat attttttgtc aaagatagcg
      181 aaaaaacgcc tgaatattgt ggtttcaggc gaataatgca acagagtatc agtgtagatc
      241 accacaaaat atttgcgtga taaaaagcta tttggcggga attatttccc tgtcgcgggt
      301 agtgatattt ttgaaaataa caccctcaga aattccagtc ttcatcttct gtttcaaccg
      361 ctttccccat cacataagag gagcctgaac cggaaaagaa atcgtgattt tcatcggcat
      421 tcggcgaaag cgcggcgagg attgccggat tcacttccgc catttctgcg ggaaataaag
      481 gttcgtagcc cagattcatc aaagccttat tggcgttgta acagagaaac gctttcacat
      541 cgtcagccca cggggtttcg gcgtacagct catcggtgta ttgcaactcg ttgtcgtaga
      601 gttccagcaa caaatcgaag gcgaaactct tcaactcttc acgttgtccc agagatatct
      661 tttccatgtt tttctgatat ttatagccta tgtagtaacc gtgtactgct tcatcgcgga
      721 taatcagacg gatcaggtct gcggtattgg tcagctttcc gcggctggaa aaatacatcg
      781 gcagccagaa accggaatag aacaaaaaag attcaagaaa cacactggcg attttctttt
      841 tcagcggatc atcaccgcga taatgttgct gaataatctg agcttttcgc tgcaacggtg
      901 cgttttcttc actccaggcg taggcggcat cgacatcttt ggtctggcac agcgtcgaga
      961 aaatcgaact gtaagagcgg gcatgaaccg cttccataaa gctgatattc gataataccg
     1021 cttcttcatg aggcgtgagt gcatcgggca tcagcgaagg cgcgccgata acattttgca
     1081 gcgtgtcgag cagcgtcagg ccagtaaaaa cgcgcatcgt cagttgttgt tctacgacag
     1141 ttaatgtctg ccaggcagga atatcgttcg acagcggcac cttttctggt agccagaaat
     1201 tgctggtcag gcgattccac acctccagat ctttatcgtc agatatcttg ttccagttga
     1261 tggcgctgat acgtgagagt ttcatagata ttccttaaag tgcacaggag acgcagcctt
     1321 caatttcagt gccttccagc gccatctgac gcaggcggat gtaatagagc gttttgatac
     1381 ctttgcgcca ggcgtatatc tgcgctttgt tgatatcgcg agtggtggcg gtatcgggga
     1441 aaaacagcgt cagcgacagc ccctgatcga catggcgagt cgcttccgcg taggtgtcga
     1501 tgatcttttc tgcgccaatt ttgtaagcgt cctgatacag cgccagattt tcgttagtca
     1561 taaacggggc agggtagtaa acgcgtcctg ttttgccctc tttgcgtatc tctactttcg
     1621 ccacaatcgg atgaatactc gacgtagcat ggttgatata agagatagaa ccggttggcg
     1681 gcaccgcctg gagattctgg ttgtatatgc cgtagcgcat cacgtcgtcg cgcagttgcg
     1741 cccacatctc acgggtaggt aacgtaatac cgctgcgggc aaacagttcg ccaactttcg
     1801 ccgttttcgg ctgccagtta ccttgcagat attggctgaa atattcacca ctggcatagc
     1861 gtgactgttt gaacccggcg aaggtttcac cgcgttcgcg cgccagcaac atcgaggtac
     1921 gcagtgcgtg ccaggtgatg gtatagaaat agagattggt gaaatccagg gcttccggcg
     1981 aaccataagc gatgccttcc ctcgccagat aaccatgtaa attcatctgc cccagtccga
     2041 tggcgtgcga ggcggcattt ccggcttcga tggacggcac gctgcggata tgactcatat
     2101 ctgacaccgc tgttaaacca cgtacggcag tctctaccgt gcgggcaaag tcgggggaat
     2161 ccatggtgtg agcaatattc aacgaaccta aattgcagga aatatcatgg cctgtacggg
     2221 catagtcgag attctcgtca tactctgagg cgctgttaac ctgcaaaatt tctgagcaga
     2281 gattactcat atttatgcgc ccggcgatag ggttagcacg gtttaccgtg tcttcataca
     2341 tgatgtaggg atagccggac tcaaactgga tttctgccag tcgctggaag aaatcacggg
     2401 cgttgaggta ttttttgcga atgcgttcat cggcaaccag ttcgtcatag tgttggctga
     2461 tggccacatc ggcaaacggc ttgccataaa ctcgctcaac gtcatagggc gaaaacagcg
     2521 ccatctgcgc attctctttt gccagatgga aagtgatatc cgggatcacc acgcccagcg
     2581 acagtgtttt aatgcggatt ttctcgtcgg cattttcccg tttcgtatcg agaaaacgca
     2641 gaatatcggg atgatgggca tgtaaataga ctgcaccagc cccctgacga gcgccgagct
     2701 ggttggcata ggaaaatgcg tcttccagca ttttcatcac cggaatcacg ccagaagatt
     2761 gattttcaat acgtttaatt ggcgcgcccg cttcccgcag attcgacagc aaaaatgcca
     2821 cgccgccgcc gcgtttcgac agttgcagtg cggaatttac cgcccggcca atcgactcca
     2881 tattgtcttc aatacgcagc aaaaaacagg aaaccagttc gccgcgctgc tgcttaccac
     2941 agttgaggaa tgtcggtgtt gcaggctgaa agcgtcctga cagcatctca tcggtcagtt
     3001 gcaacgccag tgtttcatcg ccttgtgcca gcgttagcgc caccatcgtg actcgatcgg
     3061 caaaatcttc cagataacgc ttaccgtcga atgtcttcaa cgtatagctg gtgtaaaact
     3121 tccatgcccc gaggaatgta tggaaacgaa aaccgctggt gtgtgcgtgg gcaaacagcg
     3181 taatgacaaa gtcgcgagag tagcgattaa gaacgctttc atcatagtaa ccttcgttga
     3241 ccagccagtt caggcgctgc tgctggctac tgaaggtcac actgttcgga cgcacatgcg
     3301 tcgccataaa ggcgtcaacg gcctggcggt ctttatcgaa ctgaatgcga cctgcgctat
     3361 cgtagaggtt aagcatcgca ttcagcgcgt ggtaatccat cgtttcctgc gtcaggcatt
     3421 ctgcggttgt cgttgccaaa attcggttac tcctttacga acgttttcga tatcgctttg
     3481 cgtacccatg agttcaaaac ggtacagcca cggcacgccg catttctgag caatcacatc
     3541 tccggcacgg ccatacgcct caccaaagtt gcgattaccc gaagcaataa cgccgcgaag
     3601 caacgcccgg ttgtgctcgt cgtttaaaaa gcgaattacc tgtcgtggca ccgcgccagc
     3661 cgtaccgccg ccgccgtaag agggcacgat caggatgtaa ggctcgtcta cctgaatccg
     3721 ttcccgctca ttgagcggga tgcgcaccgc gggcagacct aaacgttcga taaaacgctg
     3781 cgtattttcg gagctgctgg agaagtagac gagctggctc atgcactggc cgcgtgtggc
     3841 gctggatgca gacggttaat catgtccgga cggaaaccag accagctaag atcgccagca
     3901 atcactaccg gcaactgacg aaagccctga gcacgcaacg cttccgctgc ttcaggaacg
     3961 cgatcgacat taatcatttc aaaatcaaag ccccggtttt ccatcgcccg tttggtggcg
     4021 tggcactgaa cgcaatcgtt acgagtgtaa atagtaatgc gcatgattcg tatttccgtt
     4081 taaaatgaag atacggcgcg atgatacgcg tcgggttgtc tctctgttga tacagagata
     4141 ctagatgtag ttgaaaaaag attcaaccac acaatatata gcaaatagtg gtcgaaatta
     4201 ccctggatat gagcgtgacg gggtaggggg atttttgtga ttcaccaggc aaaaagaaac
     4261 cccgaagaca ggcttcgggg tcaaagacgc gtatttatta tcatttttgc actacgattt
     4321 gcgcatgctt aacagtgcgc cgataaaaat acctaccgca gctgctgtac ccacgctaca
     4381 ccagggtctt tcacgaacaa aagaatccgc gcagccaacg gcatcgcgcg cggcttgctg
     4441 gacgcgagta cgaccatgca ttcgcgccct ggtttctttc agcaatgcct gagctttact
     4501 gcgtgcggct tcagcttccc ctttggcgtc gctgccccag gactttaata cagattcgag
     4561 gctgtcagct aattgattga catcattctg gatatcctgc acgccatcat caacgtcgtt
     4621 gcggttcggt cggttaaaca tatgatcccc cattagaatt cgatgtaatt ttcagtgtag
     4681 accataattt tcttaactga ggttcatcgc ggctttatgg acttttctga aagcgttgtg
     4741 aatgcgcaat caggtaaggt aggcccgcgg ttgataataa cgaggatcaa atatgtattt
     4801 acgaccagac gaggtggcgc gcgtacttga aaaagtcggt tttactgtcg atgtggtaac
     4861 ccagaaagcg tatggttacc gccgtgggga aaattatgtc tatgttaatc gcgaagcgcg
     4921 aatgggacgt accgctctgg taattcatcc aacattaaaa gaacgcagtt caacactggc
     4981 tgaacccgct tccgatatta aaacttgcga tcattaccag caatttccgc tctatttagc
     5041 aggcgagcga cacgagcatt acggtatccc gcatggcttc agttcgcgtg ttgcgcttga
     5101 acgttatttg aatggtttat ttggcgaagc cagttaaaga cgcgactggc gatgccagtc
     5161 gcgaaaagaa gagtcaggct tttacctgct ggtaacggct gactttaaac agtcggcggc
     5221 aataatcgag gaagtagcca taaaccgccc ccatcaacat cgaaacaacg atgtttgaac
     5281 tgaccgccgc cataatctga tgccagtctg cgcccaccac taacaagatc gccacataca
     5341 ccggtgactg gaacgtcaca taagccagga tatcagccag attttttatc cagcccgacg
     5401 ggctaacttt gcgtgccgcg cgcataaaca gatcgcggta cataccgtat ggccatgcaa
     5461 ttaagatgtt caccggaatc gctaccaatc tggaataaaa agactgttcg aagctcattc
     5521 cggagaggaa aacttcaata cacatgttca cgacagaaca gtaaacaacc atcgcgaacg
     5581 tatctgcaac tgcatgacgc aagcgtgact gcggtgagaa catgctaatg ctccttaata
     5641 aaagcgtaat gggatgttaa tggagatgaa agcagtggat ctcattgcca tctgcatttt
     5701 cgcatagcgt atatcgctgg ctatgactaa tcaattagtg aaaaactttt atttagtcag
     5761 ttttt
//
LOCUS       DBANUU010000218         5684 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000218 DBANUU010000000
VERSION     DBANUU010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.221"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(64..630)
                     /locus_tag="ACIE68_004464"
     CDS             complement(64..630)
                     /locus_tag="ACIE68_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004145277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFX5910660.1"
                     /translation="MRLFGYARVSTSQQSLDIQIGALKNAGVKANRIFTDKASGSSSA
                     REGLELLRMKVEDGDVILVKKLDRLGRDTADMIQLIKEFDALGVAVRFIDDGISTDGE
                     MGKMIVTILSAVAQAERTRILERTNEGRLEAKSKGIKFGRKRKIDRNKIKVMHDKGTG
                     ATEIAKHMGIARSTVYKIINSESSPIND"
     gene            794..3800
                     /locus_tag="ACIE68_004465"
                     /pseudo
     CDS             794..3800
                     /locus_tag="ACIE68_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element ISEc63 family transposase"
     gene            complement(3834..4589)
                     /locus_tag="ACIE68_004466"
                     /pseudo
     CDS             complement(3834..4589)
                     /locus_tag="ACIE68_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
     gene            complement(4653..4913)
                     /locus_tag="ACIE68_004467"
                     /pseudo
     CDS             complement(4653..4913)
                     /locus_tag="ACIE68_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4965..5665)
                     /locus_tag="ACIE68_004468"
                     /pseudo
     CDS             complement(4965..5665)
                     /locus_tag="ACIE68_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1429 a         1302 c         1364 g         1589 t
ORIGIN      
        1 aaaagttatt gtccaaatta taacaaatat tctcacaatt tcaccttacc agttttaaga
       61 taatcaatca ttgattggtg aactttctga attgataatt ttatatacgg ttgaacgcgc
      121 aatacccata tgcttagcta tttcagttgc tccagttccc ttatcatgca taacttttat
      181 tttattcctg tctatcttcc gttttctgcc aaatttaatt cctttagact tggcctccaa
      241 tcttccttca tttgttcgtt caaggatgcg agtacgttct gcttgtgcca ctgctgatag
      301 aatggtgacg atcatcttcc ccatttctcc atcggtactg atcccatcat caatgaatcg
      361 gactgccaca cccaaggcat cgaattcttt tattagttga atcatatcgg cagtgtcacg
      421 accaagacgg tctagcttct ttaccagaat gacatctcca tcttccacct tcatgcgcaa
      481 cagttcaagt ccttcccggg cagatgaact tcccgatgcc ttgtccgtaa aaatacggtt
      541 ggcttttacc cccgcgttct taagtgcccc aatttgaata tctagggact gctgactggt
      601 tgagacccgt gcgtaaccaa aaagtcgcat aaaaatgtac cttaaatcga atatcagaca
      661 ccttgtgtct attatggcaa aaatacgatt taatagacag tccatgtttg ctgttttatg
      721 ctgtcttata atttataata tttcggacgg ttgcaaaatt gttaatgaat aactgtcagg
      781 tagggagcct gatatgcctg tcgactttct aacctctgac cagaaaaaga attacggttg
      841 ttatgctgca gaacctaatg acgtgcaact ggcgcgctat tttcatcttg atgaacggga
      901 tctggccttc attaaccaac gacggggcaa acataatagg ctgggcattg cacttcagct
      961 caccacagcc cgttttctgg gaacatttct gacggattta actcaggttc tgcctggtgt
     1021 tcaacatttt gtcgcggtac agcttaatat ccgccgtcca gaagttctct cccgctatgc
     1081 tgaacgggac actaccctta gataacatac tgcattaatt aaggaatatt acggctatca
     1141 tgaatttggt gattttccat ggtctttccg cctgaagcgt ctgctatata cccgggcgtg
     1201 gctcagtaat gagcgaccgg gtctgatgtt tgattttgcc actgcatggt tacttcaaaa
     1261 taaggtatta ctgcccggag caaccacact agtacgtctc atcagtgaaa ttcgtgaaag
     1321 ggcaaatcag cggctgtgga aaaagctggc cgcactgccg aacaaatggc aggcagctca
     1381 agtgatggag cttctggtca ttccggaagg tcagcgtgta tcaccactgg aacagttgaa
     1441 aaaaggccct gttacagtca gtggacctgc gtttactgag gcgctggaac gatatatccg
     1501 attacgaagc cttgagtttt cccgactgaa cttttccggt ctgcctgcca ttcaactgcg
     1561 taatctggct cgttatgctg gcatggcgtc ggtaaaatat atcgctcgaa tgccacagca
     1621 gagaaagctt gctgtactta ctgcattcgt taaagcgcag gaaataacgg cattggacga
     1681 tgccgttgat gtgcttgata tgctaattct ggacattatc cgcgaagcaa agaaaactgg
     1741 gaaaaaaaag actcaggacg ctgaaagatc ttgatcaggc cgcattgtta ctggcgcggg
     1801 catgtgcatt gttgctggat gataatacag atgttccaga tctcaggcag gttatcttca
     1861 agtgcgtacc caaaaacaga ctggcagaat ctgtaagtaa ggttaatgaa cttgctcgtc
     1921 cacataacaa taatttccat gacgaaatgg ttgagcagta cggtcgggtt aaacgttttc
     1981 ttccggcggt gttgcggaac ctgcatttcc gtgcggcacc ggcaggtgaa catgtactgg
     2041 ctgcgattca ttatctggca gaactgaatg gttcgaaaaa gcgcatcctt gatgatgcgc
     2101 ctgaacatat tatcaccggt ccctggaaac gcctcgtata cgatgcggag ggacggatac
     2161 agcgtgcagg ttattcacta tgtttgctgg aacgccttca ggatgcactg cgccgccggg
     2221 acatctggct tgaaagcagt gatcgctggg gagatcctcg cgagaagttg ttgcaaggtg
     2281 aagagtggca gactcagcgt attcctgtct gtcgggcact gggacatcct gtcgatggac
     2341 gtaaaggtgt gcaacaactg gctattcagc tggatgagac ctggaaagcc gtggcatcac
     2401 gatttgaaaa gaatgcagaa gttcatatct gtaatgaagg taaatatcca tccctgacta
     2461 tcagttgtct ggagaaacag gaagagccac catcgttgct tcgtctaaat aatcggatca
     2521 aacagctact cccaccggta gatttaacgg aactgttact tgagatagat gcccggacag
     2581 gatttacaca tgagtttgca catgtcagtg aatctggtgc tcgagcgcaa gatttgcaca
     2641 tcagtttatg tgcggtattg atggctgaag cctgtaatat cggactggag ccgctgataa
     2701 agcacaatat accagcgctg acccgtcatc ggctcagttg ggtgaaacag aattaccttc
     2761 gtgcagaaac gctagtcagc gccaataccc gcctggttga ttttcagtcc acactggagc
     2821 ttgctggtcg ttggggaggt ggagaagtgg catcagctga cggcatgcgt tttgtcacac
     2881 cagtgaagac tatcaactca ggatctaaca gaaaatattt tggttctgga cgaggcatca
     2941 cctggtataa cttcgtatct gatcagtact ctggttttca tggcattgtg gtacccggta
     3001 cattacggga ttcgattttt gtactggaag gacttcttga gcagcagaca gggctgaatc
     3061 cagttgagat catgacagac actgcgggta gcagcgatat tattttcggt ctgttctggc
     3121 tactggggta tcagttttcc ccccggcttg ccgatgcagg tgaagcggtg ttctggcggg
     3181 tgaataaatc ggcaaactac ggagtactgg ataagttggc ccgagggtat gcagatctgt
     3241 caaaagcgga gagtcagtgg gatgagatga tgcgaaccgc tggttcgctg aagttgggta
     3301 caattcatgc gtcagaactt attcgctctt tactgaaaag ctcgcgccca tcaggactgg
     3361 cacaggcaat tatggaagta gggcgtgtaa acaaaacgct atatctcctc aattatattg
     3421 atgatgaaga ttatcgtcgg cggatcctga cgcagctcaa tcgaggagaa ggccgccacg
     3481 ctgtggctcg tgcgatttgt tacggacaac gcggagagat cagaaagcgt tatcgtgaag
     3541 ggcaggaaga tcaactgggg gctctgggcc tggttactaa tgcagtggtt ctgtggaaca
     3601 cgctctatat gcaggaagtg ttgagctgga tgcgccgtaa tggagaagaa attatagatg
     3661 aagatatcgc tcggctatct cccctgatgc acgggcatat caatatgttg ggccattata
     3721 cattcacact gccagaggat attttaaaag gagagctgag agctctaaat ttaaatataa
     3781 acaacgaatt atctccttaa cgtacgtttt cgttccattg gccctcaaac cccgtctaca
     3841 agtgtggcta tatgagagtt ttttgtacct gagactaaat cgccctccca atgccccaga
     3901 gagcgtctgt tatcgatatt tcgggaacgt tcgtgaattg gtgttccgtt cactatgtta
     3961 atcgtacctc tttcgcattt gcgggtatga cgcctgccat ggcgaaggct atgcgaccgt
     4021 cgcagatgct gtatattcag gtggtgtagc gcttcacggc tacgaaagta cagcgtttta
     4081 taaattgtct caggtgatat tcgcagcgtt ttttgacgtg gttttgttcg ccttaaccat
     4141 cctgatattt gctctggaga ccatttcatc tccagttttt ccagaacaag ctttcgcaat
     4201 ggtaaatttt gatccagtaa gcacggtttt ggccttttcg ccattctgtt ggctcggtta
     4261 ttagcatcaa cagctttgta atagcgtctt ccccgattac gctgaacttc acgtgagatc
     4321 gtcgaaggac tgcgattcag cgcagtagct atcgcacgaa tgctcatttt ggctgacaaa
     4381 ccagctcgta tctcctcgcg ctcagacagt gtcaggtgag ctacagcccg cttacgctca
     4441 tggggtttta tgccgccagt atcccttaac atagtgaaga tcgttccggg ttttgaaccc
     4501 aggatattcg ctatttcact gaagcctgtt ccgttcttcc atagttcaaa aacagaggct
     4561 ttttcctctg ctgtaaatgt tcgtctcatt caaaaaacct ccgcaacccc atgttttcac
     4621 ataactgttg cgttgaccaa ttgaatctac agtgcttttt ttaatatatc tcgctcaagg
     4681 cgagcttcat ttaacgcctt acgcagttgc agaatttcag attccagttc agccaccgtg
     4741 cgggaaccag gagtaccgag cccttttctg gcggcggtaa cccattgtcc taaagtgcct
     4801 tcaggaagag ataatcggga agcgccttca ctgatcgaaa gttgattttc aagaaccgtt
     4861 ctgacagctt cggctttgaa ctctttagag taacgttggg tttttctgct cattattagc
     4921 tccttctgat gccattctat ttcaggaagg agtgtccgtt aaactcaggc tacctcaaac
     4981 atgtgttttt cgataaacgc tccgattact ttttcgtgga tctcaactga gcgtgagaag
     5041 cagattgttt tacgagccaa ccgcttaatg caggtgcgta gcgtcagatt attacgctca
     5101 atgcgttggg tgaatatttt gccggtcaga tgcttattct tcggcacctc ccggccatag
     5161 ctgcccagtc atcgctggtc agcatgccga tgttgaaggg tgtaagcagt gccagtagct
     5221 cccggcacgt ttgatcggtt cggggaccaa aagtgtaggc cagtacaccg cctgttttgg
     5281 tgttgtacgc gtaccagagc cagtgttgcc gggctttact gccaacgcag ctccaattgc
     5341 tcatcaagct cgcaaataag cgccacatca gcatgggcaa cagacgaaga agttattcgc
     5401 tttggcgcga gttttttaaa gtccggatga cggtgttaat atcaattttc agtgtcctag
     5461 cggtatcgcg aaccccggca ccattaaagg ccatttcagt tatcagctct ttcatacccg
     5521 gcttacgtgc ttgataagta taagtgagct gaaacacacg gtggcagtca cggcagcgaa
     5581 atctgtcacg gcctttaggg ttctgaccat ggcggtaaac ctgagctgac tgacaacggg
     5641 gacaatgaat attaactctg gccatgagat aacctcaaaa gccc
//
LOCUS       DBANUU010000219         5652 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000219 DBANUU010000000
VERSION     DBANUU010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.222"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..706
                     /locus_tag="ACIE68_004469"
     CDS             <1..706
                     /locus_tag="ACIE68_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFX5910661.1"
                     /translation="KKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFS
                     LESVFILVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVA
                     GVLSAAFYVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVF
                     SSGILQYFLAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFI
                     VTVLSVYFLNRKYSEE"
     gene            complement(1909..2100)
                     /locus_tag="ACIE68_004470"
     CDS             complement(1909..2100)
                     /locus_tag="ACIE68_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFX5910662.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENR"
     gene            2165..2488
                     /locus_tag="ACIE68_004471"
     CDS             2165..2488
                     /locus_tag="ACIE68_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFX5910663.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     ARDDS"
     gene            2697..3128
                     /locus_tag="ACIE68_004472"
                     /pseudo
     CDS             2697..3128
                     /locus_tag="ACIE68_004472"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3166..3696
                     /locus_tag="ACIE68_004473"
     CDS             3166..3696
                     /locus_tag="ACIE68_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFX5910664.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSN
                     ESLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETN
                     WTKDNGKLNAVRLVQE"
     gene            3703..3957
                     /locus_tag="ACIE68_004474"
     CDS             3703..3957
                     /locus_tag="ACIE68_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFX5910665.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDN
                     AMLNGRVMQLSQQVQHLSERTERLSERTERLSQLYSENRR"
     gene            4438..5490
                     /locus_tag="ACIE68_004475"
     CDS             4438..5490
                     /locus_tag="ACIE68_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910666.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLR
                     KWVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARR
                     GEVSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKD
                     GLKIYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALA
                     DIRNMTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPG
                     ELTKDERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSF
                     QPRKFENKPLYEKEDD"
     gene            5504..>5652
                     /locus_tag="ACIE68_004476"
     CDS             5504..>5652
                     /locus_tag="ACIE68_004476"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFX5910667.1"
                     /translation="MLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVILSWI
                     VLFIK"
BASE COUNT         1493 a         1159 c         1521 g         1479 t
ORIGIN      
        1 aaaaaaactc ccattaaact caaaagcgat acttgcttat gcgacaggcg tttttgaacc
       61 attcttagcg tatactttta cactctacgg actttatttt atttcagcag gaagcgcatc
      121 aattattttt tcacttgaat cagtgtttat attggtgttg tctgttctta tttgctcagt
      181 taaaattaat tccccgcaat attttatatt gttccttata ggtgcaattg ttggcagtat
      241 tttggtagcc ttgccagaca tcagtaatgg atcgaataat attgttggat attgtcttgt
      301 tgttgccggt gtgttatcgg cagcatttta tgttgttata tcgtctcgcc ttattaaatc
      361 gtttgagcct atcacattat tgagcggaca gttgacattc tcccttatac tgtcatcgct
      421 ctttattttt atatctaaat catcaattta tcttccgttg gaatccgtga ttattgtttt
      481 ttcatctgga atattacaat attttttagc tttttgtttt tacttgaact ccttgcgttg
      541 gctcccagtt catttggcag ggataatgtt gtactttatt ccgctagttg cattattttt
      601 gtcatggttg tttttggatg aacaattcac attcttacag ggggtaggat ttattgtaac
      661 tgtgctaagt gtatattttc ttaacagaaa atactcagag gagtaacatt ctgttcagga
      721 ttgccccccg gcctccatcg ataccgtgcc aaatgcacgt aaaaaacggg agcgtttctg
      781 gtgtcggaat gtaaccaagg gggtcagagg cgcgctaaaa cgctctcagg ggagtcaaat
      841 agagacgttt gtaacaaaaa gatcaaagga tcctcttgag atcctttttt tctgcgcgta
      901 atctgctgcc tgtaaacgaa aaaaccgcct tgcagggcgg tttgatcgaa ggttaagtca
      961 gcttgcagac tgcttaacca ggtaactgtc ttatgcgaga cgagtcacca aaattgttct
     1021 tctagtgtag ccgcactccg gcagccactt caagtactct ctaaatatct cgcacatgac
     1081 ttttaccaat ggctgctgcc agtggcgctt tgtggtgtct tttccgggtt ggacccaaga
     1141 gaacagttac cggagaaggc gcagcagtcg ggctgaacgg ggggttcctg catacagccc
     1201 agcccggagc gaactgccta agtcgccccg agataccaga agacgtgagc tatgagaaag
     1261 cgccacgtgt cccgtaaggg agaaaggcgg aaaaggtatc cggtaatcga tatggcggga
     1321 acgtgggggc gcaggaggga gcgattcacc ggaaacggtg atgatcttta agccacgtcg
     1381 ggtttcgcca ccgctggcgg atttcatggt tgagccgcaa ggctcccaca gatgcaccga
     1441 aaaagtgtct gttttgtgaa gtccgtcagg ggggcggagc ctatggaaaa agcgcctccg
     1501 gcgcggcctt tcccggggtt tcgttttttg cgcacatgtt ctttcctggt ttatccccgg
     1561 gttctgtgga taacagggtt agcgtttttg agaaaggaga gtccgctcgc cgcagccgaa
     1621 cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga gcgcctgaac tggcattttc
     1681 tccttacgca tctgtgcggc atttcacacc ggacatggcg cacttttcat acaatccgca
     1741 ctgatgccga atggttaagc cagtatacac tccgctatcg ctacgtgact gggtcagggc
     1801 ttcgccccga caccccaaag gtcaacggcc tgacgcgccc tggcgggctt gtctgctccc
     1861 ggcatccgct cacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agcggttttc
     1921 accgtcatcc ccgaagcgtg cgaggcaact gcggtaaagc tcatcggcgt ggtcgtgaag
     1981 cgattcacag atgtctgcct gttcatccgc gtccagttcg ttgagcttct ccagcagcgt
     2041 taatgtctgg cctctgatga agcgggccat gttaagggcg gttttttcct gtttggtcac
     2101 tgatgcctcc gtgtaagggg gggttctgtt catgggggta atgataccga tgaaacgcga
     2161 gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgctgtga
     2221 gggtaaacaa ctggcggtat ggatgcggcg ggtctgcctg ggtgagccgg ttgcccgttc
     2281 cgggaagctg ccgacactgg caccgccgtt actgcgtcag ctggccgcca tcgggaataa
     2341 ccttaaccag acagcgagga aggtgaacag cgggcagtgg tcttccggtg accgggttca
     2401 ggtggtggcc gcactgatgg ccatcgggga tgagctgcgc cggctgcgtc tggcggtcag
     2461 ggaacagggg gcgcgggatg atagttaagt ttcatgccag gggaaaaggt ggtggcagtg
     2521 gtccggttga ttacctgctg ggaagggagc gtaaccggga aggggcaacg gtgcttcagg
     2581 ggaatccgga agaggtccgg gaactcatcg atgccacgcc atttgcgaag aaatacacgt
     2641 caggtgttct gtcgttcgcg gagaaggagc tgccgcctgg agggcgtgaa aaagtgatgg
     2701 cgagctttga gcgtgtactg atgcccggtc tcgagaaaaa tcagtacagc atcctgtggg
     2761 tggagcacca ggacaaggga cggcttgagc tgaattttgt cattccgaac atggagctac
     2821 agaccggaaa acgcctccag ccgtactacg accgggcaga caggccgaga attgatgcct
     2881 ggcagacgct ggtgaatcac cattacggtc tgcatgaccc caacgccccg gagaaccgcc
     2941 ggacgctgac ccttcctgat aacctgccgg aaacgaaaca ggcgcttgct gagggcgtca
     3001 cacgcgggat agacgcgctt taccatgccg gagagataaa aggccgtcag gatgtgattc
     3061 aggcgctcac agaggcgggg cttgaagtgg tcagggtgac gcgaagcagt atcagcattg
     3121 cagacccgaa cggcgggagg aatatcagac tgaaaggagc gttttatgag caatctttta
     3181 cagacgggcg cggagttcga gaaaaagctg aaagagagag ccgaatctac agagaaaatg
     3241 ctgaacgacg agttcaggaa gctcgaagaa tctgtaaaca gggaactgac ctcaaacgaa
     3301 tctttaatca ggaacgctat caacgaccat acaacagccc tgaaagaact gctggagagg
     3361 tatcagaaaa cgaccgtcga tacgatggac gcgcactgga aaaccgtact gaaaatgagc
     3421 gtgaaacgct ggctgtggct gattatcgtc tcagtactga tgtttgccac gaccgggagt
     3481 ctgctgtggt accagggcat gaagataaac gcgaatatga atattctccg cgagcagaaa
     3541 gagagtctgg agaagctgaa cgcgaagaca tggggcgtgc gttatcacga ggacagcaac
     3601 ggacgttttc tggtgttgcc gaaggggatg aaagccgaaa cgaactggac gaaggacaac
     3661 ggcaaactga acgcagtgag actggtgcag gagtgacaga gcatgacgga gctggaaaga
     3721 catttgctga acgcattaga gcaactgcaa caggactata tgcagcggct gaacgaatgg
     3781 gagaacgcct tcgtggaatt gcagaaaatg ttttcgctta cgcaacggga caacgcgatg
     3841 ctgaacgggc gagtcatgca gttgagtcag caggtgcagc acttgagcga gcggacagaa
     3901 cgcttgagcg agcggacaga acgcttgagc cagttataca gcgagaacag gagataaggg
     3961 atgaacggct gatgcaggaa cgcgaacatg agttatctct ggagcgggag cgtcagccgg
     4021 aaatccagga gcggacactg gatggccctt cgctgggatg gtgaaaccat gaaaaatgac
     4081 gtgttcagtg gattaaggcg ggctaatgtg gcctgtagca tctggttgca taggtattca
     4141 tgcagttaaa atttatcagg tgcgatcgcg gcagtttttc gggtggtttg ttgccatttt
     4201 tacctgtctg ctgccgtgat cgcaccgaac gcgttttggc ggtgcgtaca atgcgacgtt
     4261 atggtaaatg gagcggattt tcaggaagga gtgatactaa caactcgaac actgtgcaaa
     4321 taatgatttt tttaaaatat cggtaacgat aataaattat ttcgttttac gccggtaata
     4381 aatataatgt agtttcagta tataaaaaaa tatatgaggt gtcttgtggg agctttcatg
     4441 aatcttcaac aacgtataaa taaactacca caattatcat ccagtttttc atttggaaaa
     4501 gatattgata atattcattc atttatcttt aacgaaactt ctaaagataa aattgaagat
     4561 ttactaagaa aatgggtgtc aggcaatcag ccctgtgtat ttggtaaatt ggcaagcaag
     4621 aaaattaaag ggcttgattt ccatttatct attgtaaata gcccccaact atataatgat
     4681 gatggtcatt tgtttgattt tttgcgtaat gaaagagttc gttttaaaga acgcgcaaga
     4741 agaggagagg tcagtgcgca tctcatttat tttattcatc cacaacttgc ctttgctcgt
     4801 ccatcagaag agttagtgga catacaaaaa tatatttgtt cgctgcatat gccagagtgc
     4861 tatccaataa aagaggatgt tatttatact gaaagcgtac catttcagga taaagatggg
     4921 ctaaaaatat ataaggctgg tgttaatgtt ttttactctt ctgctcatag aacaagaaat
     4981 catgatagaa gaataccagg gggaatactt atttctgtaa atgccccggg gcattttatg
     5041 aggctggcga tagaaaaagg tttctataaa gatcaagaac aagcgttagc ggatattcgt
     5101 aacatgacta ttcagtctgt tggtaagggt gggtattcac atccggaagg gatttctaca
     5161 acatggcatt cagaatctaa tttggatcgt tttggatgcc cagtgcacac cggtaatagt
     5221 tcttattaca gcggttttta tcatactgat gtattaattc caggagaatt aactaaggat
     5281 gaaagattgc tccatgaaat agaaaatagt gatccaatga tcttcaactg gaatgtattg
     5341 ttttatgttt cattagaaga gtttcctata gatgatcctt attatggaga gtttattggt
     5401 attcctgtag atgatgcatc tatgtttttt aactcctttc aaccaaggaa atttgaaaac
     5461 aaaccccttt atgagaaaga ggatgattaa tatgattaaa ggaatgcttt ttgctttttt
     5521 agcagcgttc tcatgggggg cagctatcgt aatgagcaaa aaaggagttg aagaactaga
     5581 tgccggaggg gtgtttttct ggcagatttg tagtgcagta atactatctt ggatagttct
     5641 ttttataaaa ag
//
LOCUS       DBANUU010000220         5596 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000220 DBANUU010000000
VERSION     DBANUU010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.223"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACIE68_004477"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACIE68_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFX5910668.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="ACIE68_004478"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="ACIE68_004478"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFX5910669.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIIINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="yfiH"
                     /locus_tag="ACIE68_004479"
     CDS             1882..2613
                     /gene="yfiH"
                     /locus_tag="ACIE68_004479"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFX5910670.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAADAKASTAFIQHGDKYLADIYLLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="ACIE68_004480"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="ACIE68_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFX5910671.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(5358..>5596)
                     /locus_tag="ACIE68_004481"
     CDS             complement(5358..>5596)
                     /locus_tag="ACIE68_004481"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910672.1"
                     /translation="AARGVHNTLSRYRVKHFFCFSLLRFSGEPRCPGGVFAVVPCQWW
                     RIIGSYSGLTSEKDKNFIVRSFFRQNILNIVFSV"
BASE COUNT         1385 a         1345 c         1528 g         1338 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt ggtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacgatc gacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgagtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagac attattatca ttaataaacc
     1201 gcgcgacctg gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccaccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgcaggt ggcacggtgg acgagccaat cagtcgccac ccgaccaaac gtacccatat
     1501 ggcggtgcat ccgatgggca aaccagcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 acatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagaaca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaag
     1921 ggtgttgcgg cttgtagctc tactcgtatc ggcggcgtga gcttgccccc gtatgactcg
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgct catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacagtttc ctgttttgct gataatccgg aaaatattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagcagacg ccaaagcgag tacagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatctgc ttgcccggca gcgtctggcg agcgttggtg ttgaacaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 gggcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttgt taacatcagc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaacaga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtataga tatgtccgag tttatggaga aacactcggt gtctcgtttg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagcggtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt cgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt atcgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcgtc gattgcgcag attcagttga aacgtctgta taaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cggtctatgg tgcacgtcct ctgaaacgtg caattcagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgccgtc cagtaaatga taaaacgagc ccttcggggc
     5341 tcgtttttgt ctataagtta gacggaaaag actatattta agatattttg cctgaaaaat
     5401 gagcgaacga taaagttttt atctttttcg cttgtcaggc cggaataact ccctataatg
     5461 cgccaccact gacacggaac aacggcaaac acgccgccgg ggcagcgggg ttctcctgag
     5521 aatctcaaca gagaaaagca aaaaaaatgc ttgactctgt agcgggaaag cgtattatgt
     5581 acaccccgcg ccgctg
//
LOCUS       DBANUU010000221         5572 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000221 DBANUU010000000
VERSION     DBANUU010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.224"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(54..2123)
                     /gene="glyS"
                     /locus_tag="ACIE68_004482"
     CDS             complement(54..2123)
                     /gene="glyS"
                     /locus_tag="ACIE68_004482"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFX5910673.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2133..3044)
                     /gene="glyQ"
                     /locus_tag="ACIE68_004483"
     CDS             complement(2133..3044)
                     /gene="glyQ"
                     /locus_tag="ACIE68_004483"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFX5910674.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3139..3438)
                     /locus_tag="ACIE68_004484"
     CDS             complement(3139..3438)
                     /locus_tag="ACIE68_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFX5910675.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQWVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3613..4608
                     /gene="wecH"
                     /locus_tag="ACIE68_004485"
     CDS             3613..4608
                     /gene="wecH"
                     /locus_tag="ACIE68_004485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFX5910676.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4650..5087)
                     /gene="yiaA"
                     /locus_tag="ACIE68_004486"
     CDS             complement(4650..5087)
                     /gene="yiaA"
                     /locus_tag="ACIE68_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFX5910677.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5133..5474)
                     /gene="yiaB"
                     /locus_tag="ACIE68_004487"
     CDS             complement(5133..5474)
                     /gene="yiaB"
                     /locus_tag="ACIE68_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFX5910678.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
BASE COUNT         1367 a         1438 c         1362 g         1405 t
ORIGIN      
        1 tagcgataat aaaaaaagcc tgccagatgg caggcttttt aataacggcg ttattactgc
       61 aacagcgaaa tatccgcaac gcgcaggaac agttcgcgca gtttctccag catggtcaga
      121 cggttgagac gcaattcttt gtcatcaacc atgaccatca ctttatcgaa gaaagcatca
      181 accggctcac gcagctctgc cagttcgacc agcgcatcct ggtaacgacc ttccgcaaag
      241 tacggctcca gcttgtcacg tagcaccaca acctgcatcg ccagtttaat ttcttccggc
      301 tctttcaggg tagaggcatt cacgcggtcg ctcagcactt cgtcagattt cgccaaaatg
      361 ttggagacac gcttgttcgc cgccgccagt gcagcagctg cttcaagggt acggaagtgc
      421 gataccgctt tcatgcgggc atcgaaatca gccggacgag tcggacgacg cgccagtacc
      481 gcctggatgg tgtcaacggt gtaaccttcg tcctgatacc aggcgcggaa gcgaccgagc
      541 ataaagtcga taacatcatc aaccacgttg gcgttagtca gcttatcgcc atacagacgc
      601 actgcttctt cggtcagcgt ttgcagatca aggttgaggt tcttctcaac gataattcgc
      661 agcacgccaa gtgcggcacg acgcagcgca aacgggtctt tgtcgccttt cggatgctgg
      721 ccgataccga agatacccgc cagagtatcc atcttgtcag caatcgccag cgcacaggct
      781 accgggttag acggcaggtc atcaccggca aagcgcggct gatactgctc attcagcgcc
      841 accgcgacat cttccgcttc gccatcgtga cgcgcatagt gcatccccat aacgccctgg
      901 gtgtcggtga actcgaagac catgttggtc atcaggtcac acttagacag caggcccgca
      961 cgggttgcgt ggttaacgtc agcgccaatc tgttcagcaa tccagccagc cagcgcctgg
     1021 atgcggtcag ttttgtcacg cagcgtaccc agctgttgct ggaacagcac ggtttgcagg
     1081 cgcggcaggt tatcttccag acgtttttta cggtcggtgt tgaagaagaa ctcggcatcc
     1141 gccagacgcg gacgaacgac tttctcgtta ccggagataa tctgctgcgg atctttcgat
     1201 tcgatgttgg caacgaagat aaagttcggc agcagtttgc cgtcgttcgc atacaccggg
     1261 aagtatttct ggtcaccttt catggtgtaa accaacgctt cagccggcac cgcgaggaat
     1321 ttctcttcga attttgcggt cagaacgacc ggccactcca ccagcgaagc cacttcttcc
     1381 agcaggcttt cgcttaagtc agcgttaccg ccaatcttac gcgctgcttc ttcggcatcg
     1441 gctttaatct tcgctttacg ttcttcgtaa tcggcgatga ctttcccgcg ctcacgcaga
     1501 atttccggat actgatcggc gttgtcgatg gtgaattccg gttcgcccat aaagcggtgg
     1561 ccgcgaatca cgcgatcgga ctgaatgccc agaatggttg ccggaatgac tttgtcgccc
     1621 agcagcaggg tcacggtgtg caccggacgc acgaagtgca cgtcgcttgc gccccaacgc
     1681 atcagtttcg ggatcggcag tttcgccaga gaagtcgcaa ccatattcgg cagcagtgct
     1741 tcggtgcttt cgcccttcac atgggcgcga tacagcagcc attcgccttt atcggtagtc
     1801 agacgctcag cctggtcaac ggtaataccg caaccacgcg cccaaccttc tgccgctttg
     1861 ctcggtttgc cttcagcgtc gaacgcctgg gcaatcgccg ggccgcgttt ttcgatttca
     1921 cgatccggtt gcgcttccgc caggttagct actttcagcg ccagacgacg cggagcagca
     1981 aaccattgaa cggtgccgtg tgcgaggcca gcgttatcca gctccgcagt aaaattcgca
     2041 gcaaaggact cagccaggct gcgcagtgct tttggtggca gctcttcagt gccgatttcc
     2101 accagaaaag ttttctcaga catagccgcc tcttacttat ctttgttgca catcgggaag
     2161 ccgagggctt cacgggaagc gtagtatgct tctgccactg ctttggtcag ggtgcgaatg
     2221 cgcagaatgt agcgctgacg ctcggtgacg gagatggctt tacgcgcatc cagcaggttg
     2281 aagctgtggg cggctttcag aatacgctcg taggctggca gcggcagcgg attttccagc
     2341 gccagcagct gctgcgcttc tttctcgtac tgctcaaagc aggtgaacag gaagtccaca
     2401 tccgcgtatt cgaagttgta agtggactgc tccacttcgt tctgatggaa cacgtcgccg
     2461 taggtggttt tacccagcgg gccgtcgctc cagaccaggt cgtaaacgct gtctacgccc
     2521 tgaatgtaca tggccagacg ttccagaccg taggtgatct cgccggtaac cggtttacac
     2581 tccagaccac caacctgctg gaagtaagtg aactgcgtca cttccatgcc gtttagccac
     2641 acttcccagc ccagtcccca ggcacccaga gtcgggtttt cccagttatc ttccacgaaa
     2701 cggatgtcgt gaatagtcgg gtccatgccc agctctttca gagaaccgag gtacagctcc
     2761 tgaatattgt ccggcgatgg cttaatgacc acctgaaact gatagtagtg ctgtaaacgg
     2821 ttggggtttt cgccgtagcg accatcggtc gggcgacgag aaggctgaac ataagcagcc
     2881 gccatcggtt ctggccccag cgcgcgcaga caggtcattg ggtgagaggt tcccgcgccg
     2941 acttccatgt ccaatggttg aacaatggtg cagccctggc gagcccagta atcctgtaag
     3001 gtcaggatca agccctggaa ggtcctggta tcaaactttt gcatattatt tcgtgctgga
     3061 tacgtgtgga tttaaaggaa gggatcagta tacccgctgg atggaagata tacagtacga
     3121 aacgggaaaa agcagggctt aacgcatgga aagatgcaaa aaatggccat ctgcgtcaaa
     3181 agaacagaca aactgctctt tacggaaggt ataaccgcgc atttcatagc ttccctggaa
     3241 ctgttcgaag gcggtgacgt cgattttttg ctcgccggtg ttataacgtt ttgccgcctg
     3301 atccttgcat aacgcttcca tattcaacga acgcagagga tcaaccttta cccattgtgc
     3361 tttctgcacg ggtgacggtg tagaacaacc cactagcacc ataagcgcca ttgccggaaa
     3421 gaaagcgttc atcatcatta aattaccgtc tggtttgcag gtgagtctta ttatttatat
     3481 cggtatagtg gctgatatct tacgaatttc cttaagcctc tataagctgt agtgataaaa
     3541 ctcagattat tccagtgcgc caactgaatg atttatcggt cagacttttt ttaaccagat
     3601 acagagacac agatgcagcc caaaatttac tggattgata acctgcgagg gatagcgtgt
     3661 ttaatggtgg tgatgattca caccactacc tggtatgtga ccaatgctca tagtgttagc
     3721 cccgtcacct gggatatcgc caatgttctg aattctgcct ctcgtgtcag cgtgccgcta
     3781 tttttcatga tatccggcta tctctttttt ggcgaacgca gcgcccagcc gcgccatttc
     3841 ttgcgtatcg gcttatgcct gattttttat agcgcaatcg cactgctcta cattgcgctg
     3901 tttacctcca tcaatatgga gttagcgctg aaaaacctgc tgcaaaagcc agtgttttac
     3961 cacttgtggt ttttcttcgc gattgcggtg atttatctgg tttcaccgct gattcaggtg
     4021 aagaacgtcg gcggaaaaat gttgctggtg ctaatggtgg tgattggcat tatcgctaac
     4081 ccaaacacag tgccgcagaa aattgacggt tttgaatggc tgccaattaa cttatatatc
     4141 aatggcgata ctttttacta cattctgtat ggcatgttgg gccgcgctat agggatgatg
     4201 gacacacagc ataaagcact gtcgtgggta agcgccgcgc tgtttgcgac gggggttttt
     4261 attatctctc gcgggacatt atatgaattg cagtggcgcg gaaattttgc cgatacctgg
     4321 tatctttact gtgggccgat ggtttttatc tgcgcaatcg cgctattgac tctggttaaa
     4381 aacacgctgg atacgcgtac cattcgcgga cttggcttaa tctcccgcca ttcattgggt
     4441 atatacggat tccacgcctt gattatccat gcgctgcgca cccggggaat tgagcttaaa
     4501 aattggccaa tactggatat tatttggatc ttttgcgcga cgttggcagc gagtttgtta
     4561 ctttctatgc tggtacaacg aatcgacaga aacagattag tgagttaagt aaaagcccgt
     4621 tcacattgga ctgaccgggc ttacgtgaat tattcgctgt attcttcctg ggtaacctgt
     4681 gtttcttttg gcgggtttat tccggcatca cgaatattct tctgcaccgc tactgcacca
     4741 aacaagctta agaagaaagc cagtccataa aaaccttttt cactgagtaa taatgtcgca
     4801 ttccacagac ctaccatcag taatgcaaca gagataataa agacagtcag gcaggtcata
     4861 taataaatgg aagtggtcgg tatgccttca tacttgtccc gaacggtctt ttgataagac
     4921 gcagcagaaa acagtcctaa caccagtacg gcaaaataat accctttttc atttaactgc
     4981 atctctgcat tccatagccc taacagatag gtaacgatac caccaacgag agctatccat
     5041 gacacaatac taaaggccgg tgaataggtt gatattttgt tgtccatcac agtattcctt
     5101 tcattcctga atgtgtaaga gctttatgtt gctcataccg agcgaaacaa acgcatttga
     5161 ccaaacaagc agacaaaaaa ggcgatgggg tagatcacca tttcataagc attgataggt
     5221 gcatttaaaa caccgataaa caagagaccg atagtgatta acgcaactaa ctggcagata
     5281 tgggtaaaaa aattatccag ttgcccgagt ttttctgcgc gaagatatgc atagttacca
     5341 aaagttgctg tcattaacac gccaagaaaa tagccttttc cacttagcaa tgggcatgag
     5401 atccatagcc caaccagata aaccagcgcc ccgacaacaa ataatagttt tgccacagtt
     5461 tttgaggttt tcataacgat ctccatatct accagcgata cattacgagt aaccaacgaa
     5521 agacaaaact gaaaaatgcc attaacaaat gattttcaga ataaattcat ac
//
LOCUS       DBANUU010000222         5479 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000222 DBANUU010000000
VERSION     DBANUU010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.226"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..773
                     /locus_tag="ACIE68_004488"
     CDS             <1..773
                     /locus_tag="ACIE68_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFX5910679.1"
                     /translation="HEPVIGIMGKSGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFR
                     LSGHGHSMVITDLPGVGESRDRDAEYEALYRDILPELDLVLWLIKADDRALSVDEYFW
                     RHILLCGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHP
                     VVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRSQAREQFTGAVDR
                     IFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFF"
     gene            1107..4229
                     /gene="agn43"
                     /locus_tag="ACIE68_004489"
                     /gene_synonym="flu"
     CDS             1107..4229
                     /gene="agn43"
                     /locus_tag="ACIE68_004489"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFX5910680.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRTGVAVALSLAA
                     VTSLPVLAADIVVHLGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKADGTIINQEGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETRINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFRIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGSLTGNGSVKKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVVEAINGATTEEGAFVQGNML
                     QAGAFNYTLNRDSDESWYLRSEERYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGIY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKASS
                     GNNDFRARGWGWLGSLETGLPFSITDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFGHG
                     SAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSINGSSAEGY
                     NSQATLNVTF"
     gene            4350..>5479
                     /locus_tag="ACIE68_004490"
     CDS             4350..>5479
                     /locus_tag="ACIE68_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910681.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFVLLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRSIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAIRQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLDNAWYERNMAGFNEKLRES
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQDIYERSYTG"
BASE COUNT         1253 a         1476 c         1592 g         1158 t
ORIGIN      
        1 gtcacgaacc cgtgattggc attatgggta aaagcggggc cggtaaatcc tcactctgta
       61 atgcactgtt tcagggggag gtcaccccgg tcagtgatgt tcacgccggc acccgggaag
      121 tgcagcgctt ccgtctgagt ggccatggtc acagcatggt tatcactgac ctgcccgggg
      181 tgggcgagag ccgggacagg gatgcagagt atgaagccct gtaccgtgac attctgcctg
      241 aactggacct ggtactgtgg ctgattaaag ccgatgaccg tgccctgtct gtggatgagt
      301 atttctggcg acacatcctg ctatgcggac atcagcaggt gctgtttgtg gtgacgcagg
      361 ccgacaaaac ggaaccctgc catgaatggg atatggccgg tattcagcct tctcccgcac
      421 aggaacagaa cattcgtgag aaaacagagg cggtattccg gctgttccgg cccgtacatc
      481 cggttgtggc cgtatcggcc cgcaccggct gggaactgga tacgctggtc agtgcgctca
      541 tgacagcact tcccgaccat gccgccagtc ccctgatgac ccgcctgcag gacgagctgc
      601 gcacggagtc tgtccgctcg caggcccgtg aacagtttac cggtgcggtg gaccggatat
      661 ttgacacagc ggagagcgtc tgtgttgcct ctgttgtccg tacggccctg cgcgctgttc
      721 gtgacaccgt ggtctctgtt gcccgcgcgg tatggaactg gatcttcttc tgaacctgtt
      781 gtggatgatc tcctccctgc ctctgagtct gctcacaaaa gcgctgtttt cgttactgtc
      841 tctcttgtcc gtgcaatagc cggataatag aataaaacga tcgataccta tttcaccgat
      901 cgttaatatc gatcgttatg ctaataataa cccctgttag caacgtgcgc agatacacac
      961 agacatgaga ttcagggaac aacagagcca cacgtcagaa actgccgtca gccggacctc
     1021 cggcactgta accctttacc tgccggtatc cacatctgtg gataccagct tttttattca
     1081 ccctcactct gattaaggaa atgctgatga aacgacatct gaatacctgc tacaggctgg
     1141 tatggaatca cattacgggc gctttcgtgg ttgcctccga actggcccgc gcacggggga
     1201 aacgcaccgg tgtggcggtt gcactgtctc ttgccgcggt cacgtcactc ccggtgctgg
     1261 ctgctgacat cgttgtgcac ctgggtgaaa cagtgaatgg cggaacactg gtaaaccatg
     1321 acaaccagtt tgtatccgga acagctgatg gcgtgactgt cagtaccggg cttgagctgg
     1381 ggccggacag tgacgaaaac accggcgggc aatggataaa agcgggtggc acaggcagaa
     1441 acaccactgt caccgcaaat ggtcgtcaga ttgtgcaggc aggaggaact gccagtgata
     1501 cggttattcg tgatggcgga gggcagagcc ttaacggact ggcggtgaac accacgctgg
     1561 ataacagagg tgagcagtgg gtacacgggg gagggaaagc tgacggtaca attattaacc
     1621 aggagggtta ccagaccata aaacatggcg gactggcaac cggaaccatc gtcaacaccg
     1681 gtgcagaagg tggtccggag tctgaaaatg tgtccagcgg tcagatggtc ggagggacgg
     1741 ctgaatccac caccatcaac aaaaatggcc ggcaggttat ctggtcttcg gggatggcac
     1801 gggacaccct catttacgcc ggtggtgacc agacggtaca cggagaggca cataacaccc
     1861 ggctggaggg gggtaaccag tatgtacaca acggtggcac ggcaacagaa acgcggataa
     1921 accgtgatgg ctggcaggtg attaaggaag gaggaactgc cgcgcatacc accatcaacc
     1981 agaaaggaaa gctgcaggtg aatgccggcg gtaaagcgtc tgatgtcacc cagaacacgg
     2041 gcggtgcact ggttaccagt acagctgcaa ccgtcaccgg cacaaaccgc ctgggagcat
     2101 tctccgttgt ggcgggtaag gctgataatg tcgtactgga aaatggcgga cgtctggatg
     2161 tgctgagcgg acacacagcc actaataccc gtgtggatga tggcggaacg ctggatgtcc
     2221 gcaacggtgg tgccgccacc accgtatcca tggggaatgg cggtgtactg ctggccgatt
     2281 ccggtgccgc tgtcagtggt acccggagcg acggaaaggc attccgtatc ggaggcggtc
     2341 aggcggatgc cctgatgctg gaaaaaggca gttcattcac gctgaacgcc ggtgatacgg
     2401 ccacggatac cacggtaaat ggcggactgt ttaccgccag gggcggcaca ctggcgggca
     2461 ccaccacgct gaataacggc gccatactta ccctttccgg gaagacggtg aacaacgata
     2521 ccctgaccat ccgtgaaggc gatgcactcc tgcagggagg ctctctcacc ggtaacggca
     2581 gcgtgaaaaa atcaggaagt ggcacactca ctgtcagcaa caccacactc acccagaaag
     2641 ccgtcaacct gaatgaaggc acgctgacgc tgaacgacag taccgtcacc acggatgtca
     2701 ttgctcagcg cggtacagcc ctgaagctga ccggcagcac tgtgctgaac ggtgccattg
     2761 accccacgaa tgtcactctc gcctccggtg ccacctggaa tattcccgat aacgccacgg
     2821 ttcagtcggt ggtggatgac ctcagccatg ccggacagat tcatttcacc tccgcccgca
     2881 cagggaagtt cgttccgaca actctgcagg tgaaaaacct gaacgggcag aatggcacta
     2941 tcagcctgcg tgtacgcccg gatatggcgc agaacaatgc tgacagactg gtcattgacg
     3001 gtggcagggc aaccggaaaa accatcctga atctggtgaa cgccggcaac agtggtacgg
     3061 ggctggcgac caccggtaag ggtattcagg tggttgaggc cattaacggt gccaccacgg
     3121 aggaaggggc ttttgtccag gggaatatgc tgcaggccgg ggcctttaac tacaccctca
     3181 accgggacag tgatgagagc tggtatctgc gcagtgaaga acgttatcgt gctgaagtcc
     3241 ccctgtacgc ctccatgctg acacaggcaa tggactatga ccggattctg gcaggctccc
     3301 gcagccatca gaccggtgta aacggtgaaa ataacagcgt ccgtctcagc attcagggcg
     3361 gccatctcgg tcacgataac aacggcggta ttgcccgtgg ggccacgccg gaaagcagcg
     3421 gcagctatgg cttcgtccgt ctggagggtg acctgctcag aacagaggtc gccggtatgt
     3481 ctgtgaccgc agggatatat ggtgctgcag gccattcttc cgttgatgtt aaggatgatg
     3541 acggctcccg cgccggcacg gtccgggatg atgccggcag cctgggcgga tatatgaacc
     3601 tgacacacac ctcctccggc ctgtgggctg acattgtggc acagggaacc cgccacagta
     3661 tgaaagcgtc atcgggcaat aacgacttcc gcgcacgggg ctgggggtgg ctgggctcac
     3721 tggaaaccgg tctgcccttc agtatcactg acaacctgat gctggagcca cggctgcagt
     3781 acacctggca ggggctttcg ctggatgacg gtaaggacaa cgccggttat gtgaagttcg
     3841 ggcatggcag tgcacagcat gtgcgtgccg gtttccgtct gggcagccac aacgatatga
     3901 cctttggtga aggcacctca tcccgtgccc ccctgcgtga cagtgcaaaa cacagtgtgc
     3961 gtgaactgcc ggtgaactgg tgggtacagc cttccgttat ccgcaccttc agctcccggg
     4021 gtgatatgcg tgtggggacc tccaccgcag gcagcgggat gacgttctct ccctcacaga
     4081 atggcacttc actggatctg caggccggac tggaagcccg tgtccgggaa aatatcaccc
     4141 tgggcgttca ggccggttat gcccacagca tcaacggcag cagcgctgaa ggttataaca
     4201 gtcaggccac gctgaatgtg accttctgat aattctgcat tgcctctctg tggtcccggt
     4261 catcatgacc gggacccgga cgggggaaaa cgtttcagtg ccacattcac tggcattcac
     4321 aataacatga tattcatcac ggagtgacta tgttacagat agtcggcgcg ctgatcctgc
     4381 tgatcgcagg atttgccatt cttcgccttt tgttcagagc attaaccagc acggcgtctg
     4441 cgctggcagg attcgtattg ctgtgtctgt tcggcccggc tttactggct ggctatatca
     4501 ctgaacgcat aacccggtta ttccatattc gctggctggc aggcgtattt ctgacgattg
     4561 ccggaatgat catcagcttc atgtggggac ttgatggtaa acatatcgca ctggaggctc
     4621 atacttttga ctctgtaaaa tttattctga ccaccgctct cgccgctggt ctgctggctc
     4681 ttcccgtgca gataagaagc attcagcaga acgggctcac acctgaagat atcagcaagg
     4741 aaattaacgg gtattactgc tgtttttata ctgctttttt ccttatggcg tgttctgcat
     4801 acgcaccatt gatcgcattg cagttcgata tttcaccctc actgatgtgg tggggcgggt
     4861 tgttgtactg gctggctgca ttagtgacgc tgctatgggc ggccagccag atccaggcgc
     4921 tgaaaagact gaccagtgcc atccgccaga cactggaaga acaaccggtg ctcaacagta
     4981 aatcgtggct gagcagtttg caaaacgatt acagccttcc tgaaacgctg acggagcgca
     5041 tctggctgac actcatttca caacggattt cccggggaga actgagggaa tttgaactgg
     5101 cagacggaaa ctggctactg gacaatgcct ggtatgaaag aaacatggcg ggtttcaacg
     5161 aaaagctgag agagagcctg tcatttaccc ctgatgaact gaaaaccctc ttccggaacc
     5221 gcctgaattt atcaccggaa gcgaatgacg attttctcga tcgttgcctg gacggcggtg
     5281 actggtaccc cttttcagaa ggccgccgtt ttgtatcatt ccaccacgtg gatgagcttc
     5341 gtatctgtgc ctcctgcggg ctgacagaag tacatcatgc cccggaaaat cataagccgg
     5401 atccggaatg gtactgctcc tctctttgtc gcgaaacaga aacactgtgt caggacattt
     5461 atgaacgttc ttacaccgg
//
LOCUS       DBANUU010000223         5394 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000223 DBANUU010000000
VERSION     DBANUU010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.227"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            101..733
                     /locus_tag="ACIE68_004491"
     CDS             101..733
                     /locus_tag="ACIE68_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFX5910682.1"
                     /translation="MTVEKDEVKTRLTPAEWAEAEAKWTSGEYTLSKLEEEYGIRRET
                     LSRHFKKRGLEKGADSVGKMVRESLKSDAELRAKARAEKIEERRTRYDGWAYALGQMV
                     MVEVTTAKREGKPLGAIEDSLKSLQRASNTLAKCFEVSSKALGMDHAENDEEEIPNLV
                     FGELTPSQVAKLRQEDDEPEIIDDELLETLEEEALSEFDATDDGSEGEDE"
     gene            733..1989
                     /locus_tag="ACIE68_004492"
     CDS             733..1989
                     /locus_tag="ACIE68_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFX5910683.1"
                     /translation="MAIPSSLSLVQLHSGQMKVFQSPHRFKVVCAGRRWGKSRLSIST
                     IIRAAAKEKKQRVWYVAPTYQMARQILWDDLQEVLPRKWVRKKNDTTMTIVLKNGSEI
                     ALKGADKPDTLRGVALHFVVLDEFQDMKADTWYKVLRPTLSSTRGGALIIGTPKGFSE
                     FHKLWTIGQNVELQRKGQWKSWQFVTADSPFVPTAEIEAAKNDMDPKSFAQEYLASFE
                     NMSGRVYYPFDRNVHVKPLQFNPRLPIWVGQDFNIDPMSSVILQPQPNGELWAIDELV
                     LFSSNTAEVCDELERRFWRWKSQVTVFPDPAGAYRQHARGESDVDIFKEKGFLRVDYS
                     KKHPPIADRVNAVNRMLMSASGDIRLYIDPKCKHLIDSLEKVIYKPGTRDMDKTGGIE
                     HSADALGYPVHRRYPVKNRVILGGSR"
     gene            2016..3590
                     /locus_tag="ACIE68_004493"
     CDS             2016..3590
                     /locus_tag="ACIE68_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFX5910684.1"
                     /translation="MELNDKQIKDLVARRHPEYEKKKEHWDFLASTYAGGRGWFTDNI
                     FRYFKEGDQEFKERVERAYRFNHTREVVNLINKYLFKEDIHRNIEEAPEQIRNFWKRA
                     TRQNASIDSFMAAIDLQSSIYGRIWVVVDSTMSGDVESVADEKKKDARAYAYWISPQQ
                     MLDVAWDDDGNMLWALIVEVARDDADPFTSTGQEYQRYRLWTQNEWYLFREEVKKGAG
                     GAGRRQAKVILEDSGAHNLGVVPVFPVDCIGESESPYFSPSLIDDIAYLDRAVANYLS
                     NLDAIIQDQTFSQLAIPVQSLLPGDENHTKVLEMGTKRVFTYDSEGGNQPFYLSPDPK
                     QAQMIITTIKTVINEIYHSVGVAGERTKQDNAQGIDNSSGAAKMYDFQRVNSLLVTKA
                     ERLERAERQIMLLVAKWMGVDLDEDHSLIAYPESFDIRGLTDEFSVAEKLSLLQAPDS
                     VRRHQMEMLIEKIFPNITEAMKKEFDKDLLNFPPKNDLNTLENKSALTYDRGAAQESG
                     QDQPRGNGDSSTQENE"
     gene            3612..4499
                     /locus_tag="ACIE68_004494"
     CDS             3612..4499
                     /locus_tag="ACIE68_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6651 domain-containing protein"
                     /protein_id="HFX5910685.1"
                     /translation="MNLWQMLLARRGLMDVAEAHERGGAGGVAADNEQSTQDPDKQGE
                     QKEQPKGDDEYAGMTQEELLAELRKTKKAGAELLKENMKRKEKERTLADQLAQYGDID
                     PARARQLLEAEQAAENARREAEQAELERRGEFDAVKKQMIEAHQAELAQRDERYAALE
                     SENASLKSQLVEMTVGASFSNSLFLRDKVLMTPAKARVIYGSHFEVGEDGSVVGYDKP
                     AGHKERAVLVDGEGKPLPFESAIERIVRADPEADALMRSEAKQGVGSHSKSTYTISQP
                     KNKSTMDKLASGLGKIGLK"
     gene            4525..>5394
                     /locus_tag="ACIE68_004495"
     CDS             4525..>5394
                     /locus_tag="ACIE68_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFX5910686.1"
                     /translation="MPLLREEAEKLSNNELEQGVIETIIDRDDLFAILPFMKINSKAY
                     LYNREKTLSEATFIDVNDTIPEGAATFEECVAKLRILAGDVDVDKFLATTMADTNNQL
                     AIQVRQKVKGLARAFRRNLILGDSSSNNKAFDGIPRLMHADQKIDIAGASMTFSMFDE
                     LVDAVKDLGADCIMMRSEHLRAYRALLRTVNVGPSEIMMENFGRPMLCHNGVPFIIND
                     FIPTDAGKASIYCLHLSEENGVTGLYGGDNAGIVVENIGTVQNKDAVRTRVKWYCSLA
                     NKHDKAIAALTNVK"
BASE COUNT         1507 a         1157 c         1433 g         1297 t
ORIGIN      
        1 agacctaatt aaatacccca gaaaacaagt tggttagcag atgcagtaaa caagttgttt
       61 tagagcgcat ggacgcgctc tgtgtgtttt agagggatct atgacggtcg aaaaagacga
      121 ggtaaaaact cgcctgacac cagcggagtg ggccgaagct gaagccaaat ggacgtcagg
      181 cgaatataca ctctcaaagc tggaggaaga gtacggcatt cgtcgtgaaa cactctccag
      241 acatttcaaa aagcgaggat tagagaaagg cgcggactct gttgggaaga tggttcgtga
      301 gtctcttaaa tctgacgcag agcttcgcgc taaagcccgt gcggaaaaga tagaagaacg
      361 tcgtacacgt tatgacggct gggcgtatgc gttggggcag atggtgatgg tcgaagtcac
      421 tacggccaaa cgtgagggta agcctttagg ggcgattgag gattctctca agagcttaca
      481 gagagccagt aatacccttg caaaatgctt tgaagtttcg tccaaagcat tgggcatgga
      541 tcatgcggaa aatgacgagg aagaaattcc gaacctggta tttggtgagc ttacgccttc
      601 ccaggtggcg aaattacgtc aggaagacga cgagcctgaa atcatcgatg acgaattgct
      661 tgagacgttg gaagaagaag ctctaagcga atttgatgcg acagatgatg gaagtgaagg
      721 ggaggacgaa taatggcaat cccgtcctcg ctcagtcttg tgcaactgca ttctggacag
      781 atgaaagtct tccagtctcc gcatcgattt aaagttgttt gtgctggtcg acgctgggga
      841 aaatcccggt tgtcgatctc cactattatt cgtgcggcgg caaaggaaaa aaagcaaagg
      901 gtctggtatg tcgctcctac ttaccagatg gctcgccaga ttttgtggga cgatctacag
      961 gaagttctgc ctcgtaagtg ggttaggaaa aagaacgaca ccacgatgac aatcgtgttg
     1021 aagaacggtt cggagatcgc cctcaaaggt gctgataagc ctgacactct gcgcggcgta
     1081 gcgttgcatt ttgtagtgct tgatgaattt caggatatga aggctgacac ctggtacaag
     1141 gtgttacgac ctactctttc atcgacacgc ggcggtgcac tgatcattgg tacgccaaaa
     1201 ggcttctcgg aatttcacaa actgtggact ataggccaga acgtagagct gcaaagaaag
     1261 ggacagtgga agagctggca gtttgtaact gccgattctc cgtttgtacc tacggcggaa
     1321 attgaagctg ctaagaacga tatggacccc aaatcgttcg ctcaggagta cctggccagc
     1381 tttgagaaca tgtccgggcg cgtttactac ccgttcgatc gtaacgtgca tgtaaaaccg
     1441 cttcagttca accctcggtt gcctatatgg gtagggcagg acttcaacat tgacccgatg
     1501 tcttccgtaa ttttgcaacc tcagccaaat ggtgagctat gggcaattga tgaattggtg
     1561 ctcttttcct ctaacacggc agaagtttgt gatgagcttg agagacgctt ctggcgctgg
     1621 aaatcacagg taacggtatt tccagatccg gcaggtgctt atcgccaaca tgctcgtggg
     1681 gagtctgacg tagacatatt caaagagaag ggattcttac gtgtcgatta ttcgaaaaag
     1741 cacccgccaa ttgcggatcg tgttaatgct gttaaccgaa tgctgatgtc cgcatctgga
     1801 gatatccggc tgtatatcga tccgaagtgc aagcatttga ttgattcact ggaaaaagtc
     1861 atctacaagc ctggaacacg agatatggat aagacaggtg gcattgagca tagtgcagac
     1921 gcattgggct atccagtaca tcgtaggtat ccagtcaaaa accgtgttat tcttggtggt
     1981 tctcggtagg taagtaatta tctaaggtta ttcaaatgga attgaacgac aaacaaatta
     2041 aggatctggt ggcgcgacgc cacccggaat acgaaaagaa aaaagaacat tgggacttcc
     2101 tcgccagcac ttacgctggc gggcgtggtt ggtttacaga caatatcttt cgttacttta
     2161 aagagggaga tcaggagttt aaggagcgtg ttgagcgtgc ctatcgcttc aaccacactc
     2221 gtgaggtggt aaacctcatc aacaaatatc tctttaaaga agacattcat agaaatatcg
     2281 aagaggcacc agagcagatc cgcaatttct ggaaacgtgc gactcgccag aatgcctcta
     2341 ttgactcatt tatggccgcc attgatttgc aatcgtcaat ctacggccgt atctgggttg
     2401 ttgttgatag cacaatgagt ggtgatgttg agtcagtagc tgacgagaaa aagaaagatg
     2461 ctcgtgccta cgcctactgg atttcacctc agcaaatgct ggatgtggca tgggacgacg
     2521 acgggaatat gttatgggcg ttaattgtgg aagtcgctcg cgatgacgca gatcctttta
     2581 cttctacagg tcaggaatac caacgttatc gtctgtggac acaaaacgag tggtatctgt
     2641 tccgtgagga agtgaagaag ggggctggtg gagcaggtcg tcgtcaggca aaagttattt
     2701 tagaggacag cggcgcgcat aatctcggcg tagttcctgt gtttcctgtt gattgtattg
     2761 gagaaagtga atcaccgtat ttcagcccat cgttgatcga tgatatcgct tatcttgatc
     2821 gtgcggttgc aaactatctg tcaaaccttg atgccattat tcaggatcag acatttagcc
     2881 agttggctat accggtacag tcgcttttac ctggtgatga aaaccacact aaagtgcttg
     2941 aaatgggcac aaagcgagtc ttcacctacg attctgaagg tggaaaccag ccgttttatc
     3001 tgtcaccaga cccgaaacaa gctcagatga tcatcactac gattaagacg gtgattaacg
     3061 aaatctacca ttcagttggt gtagctggtg agcgaacgaa gcaggacaac gcacagggaa
     3121 tcgataattc atctggtgcc gcaaaaatgt atgacttcca gcgtgtaaat agcttgcttg
     3181 tcacaaaagc agagcgtctg gaaagggctg aacgccaaat catgctactg gttgcgaaat
     3241 ggatgggggt agatctggac gaagaccact ctttaattgc gtatccagaa agtttcgata
     3301 ttcgtggcct tactgatgaa ttttctgttg ccgagaaact gtcattactt caggcaccgg
     3361 actctgtacg tcgtcaccag atggaaatgc ttattgagaa gattttcccg aacattactg
     3421 aggcgatgaa aaaggaattt gataaagatc tcttgaattt tcctccaaaa aatgatctaa
     3481 acacccttga aaataagtca gcacttactt atgatcgtgg tgcagcccaa gaaagcgggc
     3541 aagatcaacc ccgagggaat ggggactcat ctactcaaga gaacgagtga taagtaacaa
     3601 aaggaatttt tatgaatctg tggcaaatgc ttttggcccg tcgtggtctg atggatgtcg
     3661 ctgaagcgca tgagcgtgga ggcgctggcg gtgtagctgc tgataatgag cagagtacac
     3721 aagatcctga caaacagggt gaacaaaaag agcagccgaa gggcgatgac gaatacgctg
     3781 gcatgactca ggaagagtta ctggccgaac ttcgtaaaac caagaaagct ggtgctgaac
     3841 tgctgaagga gaacatgaag cgcaaagaga aagagcgcac attggccgat cagcttgctc
     3901 agtacggtga tatcgatccg gctcgtgctc gccagctttt agaagctgaa caggccgcag
     3961 aaaacgcacg tcgtgaggcg gagcaagctg aactggagcg tcgtggtgag ttcgatgctg
     4021 ttaaaaaaca gatgatcgaa gcacaccagg cagagctggc acagcgtgac gaacgttatg
     4081 cagcactgga aagtgaaaac gcatcactga aatctcaatt agtcgagatg accgtgggcg
     4141 cttccttcag taactctctc ttcctacgtg acaaagttct gatgaccccg gcaaaagccc
     4201 gcgtgatcta cggttctcat tttgaagtgg gtgaagacgg tagcgtagtg ggttatgaca
     4261 aaccggcagg tcacaaagaa cgagctgttc tggttgacgg tgaaggtaag ccgttgccgt
     4321 ttgaatccgc gattgaacgc attgtgcggg cagatccgga agctgacgca ttgatgcgta
     4381 gcgaagccaa gcagggtgta ggctcacatt ccaaatcgac ctacacaata tcccaaccga
     4441 agaacaagtc gactatggat aagttggcct ccggtctggg gaaaattgga cttaagtaac
     4501 atctaaatca aagggaattg atagatgcca ttactgcgtg aagaagctga aaagctgtct
     4561 aacaacgaac ttgaacaggg tgtgatcgaa actatcatcg atcgcgatga cctgtttgcc
     4621 atcctgcctt ttatgaaaat taattcaaag gcatatctgt acaaccgtga aaaaacgctg
     4681 agtgaagcta ccttcatcga cgttaacgac accatccctg aaggtgcagc aaccttcgaa
     4741 gaatgcgttg cgaaactgcg tattctggct ggtgacgtag acgttgataa attcctggcg
     4801 accactatgg ccgacaccaa caaccagttg gctattcagg ttcgtcagaa agttaaaggt
     4861 ctggctcgtg cgttccgtcg caacctgatt ttgggtgact ccagctccaa caacaaggcg
     4921 tttgacggca ttccacgcct gatgcacgca gatcagaaga tcgatatcgc cggtgcatct
     4981 atgactttct ctatgttcga cgaactggtc gatgcggtga aagatcttgg tgctgactgc
     5041 atcatgatgc gttcagagca cctgcgtgct taccgtgcgc tgctgcgtac agtaaacgta
     5101 ggtccgtctg aaatcatgat ggagaacttt ggtcgtccga tgctgtgtca taacggcgtt
     5161 ccgttcatca tcaacgactt catcccgacc gatgctggca aagcaagcat ctactgcctg
     5221 catctgtcag aagagaacgg cgttactggt ctgtatggcg gcgataacgc aggtatcgtt
     5281 gttgaaaaca ttggtactgt acagaacaaa gacgcagtac gtacccgtgt gaagtggtac
     5341 tgctctctgg cgaataagca tgataaggct atcgctgcac tgaccaatgt aaaa
//
LOCUS       DBANUU010000224         5372 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000224 DBANUU010000000
VERSION     DBANUU010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.228"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(1..356)
                     /locus_tag="ACIE68_004496"
                     /pseudo
     CDS             complement(1..356)
                     /locus_tag="ACIE68_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            658..2214
                     /locus_tag="ACIE68_004497"
     CDS             658..2214
                     /locus_tag="ACIE68_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012884439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFX5910687.1"
                     /translation="MHDYLLFLLGLTPLLAMIILILKVKMPIHYSVLSTLLLTLILGG
                     VFWQTPVMDMGSAVVYGAIKGLWPIVIVILAAIYSYNLMNATKSMDILRNVLASVSDD
                     KRIQVLLISWCFGGFLEAAAGYGTAVAIPIGILIALGFNPLKAAIASLVANTVPTAFG
                     AVGIPVSVLAEQVNLPVTTLSGTIILQLALFNILLPFVIVTIIGGGMKAIRGVGLITL
                     MCGISTLIPQYLVAVNLGAELPAFAGSLVSLIVIVFMAKNRKEKTEPEFLIESSADSA
                     SALSYSRGALLRACSIYILIFLFILLCSPLFPTIKSAVSQIASSLTFSLSNGSNLSMK
                     IEWIATPGVLIIIASVIGGLIQKASLSCLLNVFVSTVKQLKNSIIAISAIVAMATVMD
                     VCGLIGTLAQSLVDLTGGAYLFIAPVIGALGTFVTGSDTNSNILFGKLQTTAAEKLHV
                     DPLWLAAANTSGATGGKMISPQSIAIAVSATRMDGQGSVIMSGTLKYCFAYIVILGLK
                     VGLVYHLFMA"
     gene            2264..2983
                     /locus_tag="ACIE68_004498"
     CDS             2264..2983
                     /locus_tag="ACIE68_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFX5910688.1"
                     /translation="MNVNFFVTCIGDAFKSRMAQKSVLLLEQLGCNVHFPEKQGCCGQ
                     PAINGGYVRDAIPGMKNLISSLEENDWPVVSPAGSCTYTIKNYARYLSEEPEWAARAE
                     KVAERLHDLTSFIVNHLGITDVGAKLNGRAVYHPSCSLSRKLGVTREPLLLLENVQGL
                     ELLPFHAQETCCGFGGTFSVKMAEISGEMVKEKVGHIMDVKPDFLIGADASCLLNIGG
                     RLNREGHPVKVMHIAEVLMHR"
     gene            2994..4409
                     /locus_tag="ACIE68_004499"
     CDS             2994..4409
                     /locus_tag="ACIE68_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFX5910689.1"
                     /translation="MSLKTSDIRFKERIRIQIQDPIMRKAVANAQERIGTNRQKMVDE
                     LGHWEEWRSRAEQIRRHVLENLDAYLYQLSEKVTANGGHVYFARTREDATSYILKVAK
                     EKNARKVVKSKSMVTEEIGMNAVLQKAGIPVIETDLGEYILQLAHEPPSHVVVPAIHK
                     DRHQIRRVLHEHLGYDGPETPEAMTLFIREKIRQDFLSAEVGVTGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFQEVDVLITMLARSAVGARLTGYNTWLTG
                     PREEGHVDGPEEFHLVIVDNGRSEILGSEFSDVLRCIRCGACINTCPAYRQIGGHGYG
                     SIYPGPIGAVISPLLGGYEDFKDLPYACSLCTACNGVCPVKIPLATLIQKHRQVMAES
                     EITPLTERRITRAFNYINSHPALWKVGMVAGAKTARLFIRKGKTPISVGAIKEWTEAR
                     DLPSPDGESFRSWFKRHKKEG"
     gene            4413..5111
                     /locus_tag="ACIE68_004500"
     CDS             4413..5111
                     /locus_tag="ACIE68_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFX5910690.1"
                     /translation="MENREEFLVAIARKLGRPVRHIPEAMPEPVNTLATTRLTELTSD
                     QRCEAFIKFASEVMLAECVLVSPENAPSTALDICWKFGSGPVIISNDQRLVDTGITPL
                     LQKEMDAALWDPEKGDENIHLAEKAKTGVVFAEYGLTESGGIVLFSAPERGRSVSLLP
                     EASLFVLRKSTILPRVAQLAQKLHDMAQEGQRMPSCINLIGGPSSTADIELIKVVGVH
                     GPVHATYLIIDDCD"
BASE COUNT         1421 a         1173 c         1303 g         1475 t
ORIGIN      
        1 agattgtggt tcgagttcgt agtcgctctg aacttgcgtt tctgcttaca gcgtagcctt
       61 agctccttac gaagacgtgc cagtcggtca cgaccaacga tgatgccatt ctctgccagc
      121 tccgtctgga gccgccgggt tccatatgtt tcgcgagtgc ggatatgtgc caccgtaatc
      181 tccagtttta gccgctcatc actttgtttt ctgtctgagg gttcatgctg tacccggttg
      241 taataaccgc tcctggatac accaaatacc tgacacatcg cttcaatggg aaattgttgt
      301 cgccattgtt cgattaacgc gtatttttca gcgactcctg tgcaaaatac gctgttgtag
      361 attcaatctg tcaatgcaac accagtctta aggttatatc gggatagcac acaagtgtta
      421 cacgcataaa caaaaaatac ccaaaaagta ttatttcata acatttattc atggaagata
      481 acaatgaaac gattcagcta tattttttaa actaaaaaat actttataag aacatcccaa
      541 ttttggaggc gatactcatt cttcccctga taaataaaca ctctcgtccg acagggcgtg
      601 gaaactatca ataaccacag taatgatgct gactttgttt tcgtttaagg aaatgacatg
      661 cacgattacc tgcttttttt actgggactt accccccttc tggcgatgat cattttgatt
      721 ctgaaagtca aaatgcccat acactattct gtgcttagca ctctcctgct cacgctaatc
      781 cttggagggg ttttctggca gacgccagtt atggatatgg gatctgctgt ggtatacggc
      841 gcaatcaaag gtttgtggcc gattgtgatt gtcatccttg ctgccattta cagctataac
      901 ctgatgaatg cgacaaaaag catggatatt ctgagaaatg ttctggcgag cgttagtgac
      961 gataaacgca tccaggttct tcttatctca tggtgttttg gcggatttct tgaggcggcc
     1021 gccggatacg gtacagcggt agcaatcccc ataggtatac tcattgcact gggctttaac
     1081 ccactaaaag cagcgattgc atcactggtt gctaatacag tccctacagc gtttggggca
     1141 gtcggtatac ctgtctccgt gcttgctgaa caagttaatt tacctgtaac cacactcagc
     1201 gggacgatta ttcttcagct ggcactgttc aacattctgc tccccttcgt tatcgtcacg
     1261 attatcggtg gaggtatgaa agctatacga ggcgtagggt taattaccct gatgtgcggt
     1321 attagcacat tgatacctca gtatctggtg gctgttaatc ttggcgcaga gctcccggct
     1381 ttcgcaggaa gtcttgtcag tctgatcgtt atagtcttta tggcaaaaaa ccgtaaagaa
     1441 aaaacagaac ctgaatttct tattgagagt tcagcagata gcgccagcgc actttcatac
     1501 tctcgtgggg cgttgttacg tgcgtgctca atctacattc tgatctttct gttcattctg
     1561 ctttgctctc cactgttccc gacgataaaa agtgcagtgt ctcagattgc ctcgtcactc
     1621 accttctctc tgagtaacgg cagcaatctg tcgatgaaaa ttgaatggat cgcaaccccc
     1681 ggtgttctga ttatcattgc cagtgttatt ggcggcttga tacagaaagc atccctgtca
     1741 tgcctgttaa acgtatttgt cagcacggta aaacaactta aaaactccat tattgccatt
     1801 tctgcgattg ttgccatggc aacggtaatg gatgtctgtg ggttgattgg cactctggca
     1861 cagagccttg tggatctgac tggtggggcc tacctcttca ttgctccggt tatcggcgct
     1921 cttggtacat ttgttactgg tagtgacaca aactccaaca ttctgtttgg taaattgcag
     1981 actactgcgg ctgaaaaatt gcatgtggac ccgctctggc tggctgccgc aaatacatct
     2041 ggcgcaacag gcggcaaaat gatttctcct cagagtattg ctatcgctgt ttcagccacc
     2101 cgtatggatg gtcagggcag cgtaatcatg tcaggaacac tgaagtactg ctttgcttat
     2161 attgttattc ttggactgaa agtaggcctt gtctaccacc tgttcatggc ctgagaaaaa
     2221 acaacaaaag gacgtggaat attaatcaac aggagaaatt tctgtgaatg ttaacttttt
     2281 cgtcacctgc attggtgatg cgtttaaatc ccggatggca caaaagagtg tgctgttact
     2341 ggagcaattg ggttgcaatg tccatttccc cgaaaagcag ggatgttgtg gtcagccagc
     2401 gataaatggc ggttatgttc gtgatgccat cccgggcatg aaaaatctca tatcttcgct
     2461 ggaagagaat gactggccgg tagtatctcc tgccgggtca tgcacttaca cgataaaaaa
     2521 ttacgctcgc tatctcagtg aagaacctga gtgggcggca cgagctgaaa aagtggctga
     2581 acgcctgcat gatttgacat catttatagt aaatcatctt ggcatcacag atgttggggc
     2641 aaaactcaat ggtcgcgctg tgtaccatcc ctcctgcagc ctttcccgta agctgggtgt
     2701 aaccagagag ccgcttttgc tacttgagaa tgtacagggg cttgagctac tgccgtttca
     2761 cgctcaggaa acgtgctgcg ggtttggcgg cacattctct gtaaaaatgg cggagatctc
     2821 tggtgaaatg gtgaaagaga aagtcgggca tattatggat gttaaacctg attttcttat
     2881 cggggctgac gccagttgcc tgctaaacat cggcggtcgg cttaaccgtg aagggcatcc
     2941 tgtaaaagtg atgcatattg ctgaagtcct gatgcacaga tgaggtaaac agaatgtctt
     3001 tgaaaacaag tgatatcagg tttaaagagc gtatccgtat tcagattcag gacccgatca
     3061 tgcgaaaagc tgtggctaac gcacaggagc gcatagggac taatcgacag aaaatggttg
     3121 atgaactggg acactgggag gaatggcgtt cacgagctga acagattcgt cggcatgttc
     3181 tggaaaatct tgatgcttat ttgtatcaat tgtccgaaaa ggttactgcg aatggcgggc
     3241 atgtatattt tgcccgtacc agagaggatg caaccagtta catactgaag gtggcgaaag
     3301 aaaaaaacgc caggaaggtt gttaaatcca aatcaatggt gactgaggaa attggcatga
     3361 atgccgttct tcagaaagcc gggatacccg tgattgaaac ggaccttggg gaatatattc
     3421 ttcagctagc acatgagcca ccatcgcatg tggttgtgcc ggcaatacat aaagatcgcc
     3481 atcaaatccg cagagtttta catgaacatc tgggatatga cgggcctgaa actccagaag
     3541 caatgacatt gttcatccgt gagaaaatca gacaagattt tctgagtgct gaagttggcg
     3601 taacggggtg taactttgcc gttgcagaaa cagggtcagt ctgccttgtt acaaacgaag
     3661 gcaacgcacg gatgtgcacc acattaccta aaacacatat tgctgttatg ggtatggagc
     3721 gtattgctcc gacattccag gaggtggatg ttctgattac catgcttgca aggagtgctg
     3781 tcggggcgag actgacaggt tataacacct ggcttacagg ccccagagaa gaaggacatg
     3841 ttgatggtcc ggaagagttc catctcgtta ttgttgacaa tgggcgttca gagattctcg
     3901 gctcagaatt cagcgatgta ttgcgatgta tccgctgcgg tgcctgcatt aatacctgcc
     3961 cggcttatcg tcagattggt gggcatggtt atggttcaat ctacccgggg ccaatcggcg
     4021 cggttatttc cccattgctt ggtggatatg aagactttaa ggatttaccc tatgcctgct
     4081 cattgtgcac tgcctgcaat ggggtatgtc ctgtaaaaat acctctggca acgctaatac
     4141 aaaaacatcg ccaggttatg gcagagtctg aaatcacacc attaacggaa cgccgtataa
     4201 ccagagcatt caattacata aacagccatc cggcgttgtg gaaggtaggt atggttgctg
     4261 gggccaaaac agcccgtctg ttcatcagga aaggaaaaac gccgattagt gttggagcga
     4321 taaaagaatg gactgaagcc agagacctgc catcacctga tggagaaagc ttccgtagtt
     4381 ggttcaagcg acataagaag gaggggtaat ttatggaaaa cagagaggaa tttctcgttg
     4441 cgatcgccag aaagcttggc agaccagtaa gacatattcc cgaagcaatg ccggagcctg
     4501 taaatacgct ggctacgaca cgtttaacag agttgacatc agaccaacgc tgtgaggcgt
     4561 ttattaaatt tgcctcggaa gtgatgcttg ccgagtgtgt tctggtgtcg cctgagaacg
     4621 caccatcaac agccttagac atctgttgga agttcggttc cgggccagtc attatcagca
     4681 acgatcaacg actggtcgat acaggaatta cgccattgct acaaaaagaa atggacgctg
     4741 ctctgtggga tccggagaag ggcgatgaaa atatccatct tgctgaaaag gcaaaaactg
     4801 gtgtcgtttt cgctgagtat gggctgacag aatcaggagg tattgtctta ttctccgcac
     4861 cagaacgtgg gcgctcagta agccttttgc ctgaagcatc attatttgtt ttacgtaaaa
     4921 gcaccattct ccccagagtg gcgcagcttg cgcaaaaact tcatgatatg gctcaggaag
     4981 gacaacggat gccgtcctgt ataaatctga ttggaggccc aagttctact gcagatatag
     5041 aactgatcaa agttgttggc gtacatggtc ctgtacatgc aacctatttg atcattgatg
     5101 attgcgatta acgattgcta ataaaaaaat ggccttaagg tgatatttcg tcttgtgaaa
     5161 aggcaacaat agactaaagg ttaggcataa ttgtgtttct ttatgcctcc gcagcaattt
     5221 gtggaggctt ttttgcttac tccaaacagt ttggcttctt gaaaaagtca tatcccaaac
     5281 ctatgtaacc ggctcattta aaccgtctgg tctgtttcct ccggctctac aaaaataatg
     5341 tccatcattt ttaatggaca ctatcgtatg aa
//
LOCUS       DBANUU010000225         5366 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000225 DBANUU010000000
VERSION     DBANUU010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.229"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..369
                     /locus_tag="ACIE68_004501"
     CDS             <1..369
                     /locus_tag="ACIE68_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFX5910691.1"
                     /translation="AKHNRQDVLCCVSCGCPLTLQRGNDGQPPWFEHDQMTVAEKILL
                     RCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVMCDEDYEGEKCCPRCGTGVYSRA
                     WGRQEVPSEDARADNPLQRL"
     gene            724..1104
                     /locus_tag="ACIE68_004502"
     CDS             724..1104
                     /locus_tag="ACIE68_004502"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFX5910692.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            1101..1448
                     /gene="tnpB"
                     /locus_tag="ACIE68_004503"
     CDS             1101..1448
                     /gene="tnpB"
                     /locus_tag="ACIE68_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFX5910693.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            1498..2883
                     /locus_tag="ACIE68_004504"
     CDS             1498..2883
                     /locus_tag="ACIE68_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFX5910694.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(3122..4480)
                     /locus_tag="ACIE68_004505"
     CDS             complement(3122..4480)
                     /locus_tag="ACIE68_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFX5910695.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKVGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            4762..4893
                     /locus_tag="ACIE68_004506"
                     /pseudo
     CDS             4762..4893
                     /locus_tag="ACIE68_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1247 a         1401 c         1444 g         1274 t
ORIGIN      
        1 gcgaagcaca accgacagga cgttttgtgc tgtgtgtcct gtggatgccc gttaacactt
       61 cagcggggca atgacggaca accaccgtgg tttgaacatg accagatgac tgtcgctgaa
      121 aaaatcctgc tgcgatgtac ctggcttgac ccggcagaga aagaggcccg tcgtttgcat
      181 ctgcagggca tgacggttcc ggattatacg gtgaaggtga gaaagtggtt ttgtgtgatg
      241 tgtgacgaag attatgaggg ggaaaagtgc tgcccacgct gcggtaccgg ggtatacagc
      301 agggcgtggg ggcggcagga ggtgccgtcg gaagatgcca gggctgataa tccgttacag
      361 aggctgtaat ggttgcctcc ggagctgttt gcggtgatgc ctttcccggg aatggattgc
      421 cagtgcgggg actgtggagc ttaattccgg tgttggtggc ctttccattg attttatgcc
      481 aacagccccc tgtgactgac aggctgcctc gtcattccat tcgtatctcc agtaacaggg
      541 ggttgtattg tattttattg taccggttca ggtgtgcgtt tcccggcgcg tctgcaccgg
      601 cttaaccaaa ttcaacaggg ataaaatagt ggtaagcgta cagcctgaac cgtctggtca
      661 gaatctgacg aattagacaa agtggtgtcc accaaataag tagtgggaac caaagtgtca
      721 gatatgcaga aaaatgtgac tcccggcagg cgaaaaggct gccctaatta tcctcccgaa
      781 tttaaacagc tgctcgttgc tgcctcctgt gaacccggga tatccatctc aaaacttgct
      841 cttgaaaatg gcattaacgc caatctgttg ttcaaatggc gacaacaatg gcgcgaggga
      901 aagctgctat taccttcttc agagagcccc cagctacttc ctgtgactct cgatgcagct
      961 gccgaacagc cagaatcgct cgcagaggac ccggaaaccc tcagtatcag ctgtgaggta
     1021 acgttccggc acgggacgct ccgcttcaat ggcaatgtca gcgaaaagct cctgactctg
     1081 ctgatacagg aactgaagcg atgatcccgt taccttccgg gaccaaaatt tggctggttg
     1141 ccggtatcac cgatatgaga aatggcttca acggcctggc tgcgaaagta caaacggcgc
     1201 tgaaagacga tcccatgtcc ggccatgttt tcattttccg gggccgcagc ggcagtcagg
     1261 ttaaactgct gtggtccacc ggtgacggac tgtgcctcct gaccaaacgg ctggagcgtg
     1321 ggcgcttcgc ctggccgtca gcccgtgatg gcaaagtgtt ccttacgcag gcgcagctgg
     1381 cgatgctgct ggaaggtatc gactggcgac agcccaagcg gctgctgacc tccctgacca
     1441 tgctgtaaat ctctttatcc tggttgtcac agaataagcc cggtaaaata cgggcttatg
     1501 aacgacatct cttctgacga catcttcctg ctaaaacagc gcctggccga acaggaagcg
     1561 ctgatccacg ccctgcagga aaagctgagc aaccgggagc gcgaaataga ccatctgcag
     1621 gcgcagctgg ataaactccg ccggatgaac ttcggcagtc gttccgaaaa agtctcccgc
     1681 cgtatcgcac aaatggaagc cgatctgaac cggcttcaga aagagagcga tacgctgact
     1741 ggtagggtgt atgacccggc agtacagcgt ccgttgcgtc agacccgcac ccgtaagccg
     1801 ttccctgaat cactaccccg tgacgaaaag cgactgttgc ctgcggcgcc gtgctgcccg
     1861 aactgcggcg gttcactgag ctatctgggc gaggataccg ccgaacagct ggagttgatg
     1921 cgtagcgcct tccgggttat ccggacggta cgggaaaaac atgcctgtac tcagtgcgat
     1981 gccatcgtgc aggcacctgc accttcgcgg cccatcgagc ggggtatcgc cggaccgggg
     2041 ctgctggccc gcgtgctgac ctcgaagtat gcagagcaca ccccgctgta ttgccagtca
     2101 gaaatatacg gccggcaagg tgtggagctg agccgttcac tgctgtcggg ctgggtggat
     2161 gcatgctgcc ggctgctgtc tccgctggaa gaggcgcttc atggctatgt catgactgac
     2221 ggcaaactcc atgccgatga taccccggtc caggtactgc tgccgggtaa taagaagacg
     2281 aagaccgggc ggttgtgggc gtatgttcgt gatgaccgca atgccgggtc agcgttggca
     2341 cctgcagtgt ggttcgctta cagcccggac agaaaaggca tccatccgca gactcatctt
     2401 gcttgcttca gcggtgtgct gcaagcggat gcgtacgccg ggttcaacga gctgtatcgc
     2461 aatggtggga taacggaagc tgcctgctgg gctcatgccc gccgaaagat ccacgatgtg
     2521 cacgtccgca tcccgtcagc actgacggaa gaagccctag agcagatcgg tcagttgtac
     2581 gccatagagg cggatataag gggaatgccg gcagagcagc ggcttgctga acgtcagcga
     2641 aaaacgaaac cgctgttgaa atccctggaa agctggttgc gtgaaaagat gaagaccctg
     2701 ttcttcggct ctggccatgg tggtgagcgg ggagcgctac tgtacagcct gatcgggacg
     2761 tgcaaactga atgacgtgga tccagaaagc taccttcgcc atgtgcctgg cgtcatagca
     2821 gactggccgg tcaaccgggt cagcgaactg cttccgtggc gcatagcact gccagctgaa
     2881 taacacatcc ccgtcaatac ggccctcgct gtacgcttac ttttgttcgg actgcttatg
     2941 gcctttttcc actggtgtag caggaaagtt aaccggctgc caccatgaga tgacgtgtaa
     3001 accgtgcagg catgttttca cgtggtgacg ctttcattgg gggtctacgc cggaacccca
     3061 atattgttca catacatgaa tttgaaaaac taagcgatac tgctgtccgt accttataaa
     3121 cttatttcaa cgcttccggc agcgtcacaa tccacagttc actgtcagac tccggtttct
     3181 ccagtggctt cactctgagg gtcgtttcaa cctgcttgtc gtcaagcgtc agctttcctt
     3241 ccgcgttcac ccgataatcc ttgaatgtct tgccctcgac cggatgttgc atcgccacct
     3301 tcacgccaaa gtcgttatcg ttcgccaccg ccagcgtttt gctgtcgatc agcgccaacc
     3361 cttcagcctt ctcctgctgc cagcccaagg agcgcagatc aacaacctgc gttttctgcg
     3421 caagggtaat gccgcgctgt gacagggttt tctcatcatc aaactccgga tactcacccg
     3481 gcttgtcgaa gcctgacagg tcgctcgcct tattgagatc gacctcgtaa atcaggttac
     3541 gcattctgtt gtttttatct cttccctgtt cgatcagcag gatgtgctgg ttatcgagcg
     3601 ccacgatgtc tccgatttta gcgtcgctgt ttttgctgta ggccgcgctg tcgataggat
     3661 aaccgtacat cgcggttttt ccgcttgccg gatcgaagct caccagacgc gtaaacagcg
     3721 cctttttctt gctcttagcg tcgatatcca gcgtactttg cacggcgaca ataatacgcc
     3781 cgtccggcat acgggtgagg ccttcaaagc cgcggttcgc ctgacgccat ttgaggatat
     3841 ttggcaaacc gcccgcaatc gctttttccc cttccgccgc ctgcggaccg tggattgcca
     3901 ggattttccc tttactgtcg atgttaatca ggaacgggcc atactcatcg cacagccagt
     3961 aacctacttt accatccgga gtgatccctt ccgtatccag cccgcgatta tcccccttca
     4021 gtctatgtaa cgtgtcgctg aaggccactt cattggtgga gccaatcacg tcgcttgcca
     4081 gcggcagacc gttgatcgcg cctttatcgt catgcagagg acggggatcg atggcctccg
     4141 ctttgccgtt ttgtacacga atcgtcatca gcagcggcgc gaaatctggc gtaacgaaaa
     4201 ttttagtttc gtttttcccc tctttcggtg aatccgcgtt cggaccgcga tcggtaatgg
     4261 ttgcgaatgt cagggcatcg ccctgtttac cggtgaacag caggccagaa cctatcccaa
     4321 ccggaaggcc gttcgggaat gcactggcaa atgcgccggc gtagttcacg tgcgtgcctt
     4381 ctggaaaact gacgacatag cgctcagccg taggttgtgc cgccagggca gagaaagaga
     4441 gcgtacaacc gattagcacg ggaataattt ttcttttcat attgttagca tccataaagt
     4501 gaagatcaca ctacaccata aagaagaaac gagtcaggaa aatgacatcg aatggcaaca
     4561 tgcgcgggcc tgaggccaaa gaaatgtaaa acactgttgt tatttgtaac aacggaaaca
     4621 tttacggaaa ttatttgtct ttcttcgtga tcaaaaatgc tgtgtcttgt tggattggag
     4681 tgatgagggc tgcgtttagg aaacgcccta tattgtacgg ttgcattatc gcggtagggg
     4741 taatgcacgt gtcagtatga tgagctgacc acactcacgt gggtggactg gtataacaat
     4801 cgacgattgc tggaaaggca gggccatatc ccaccggcag aagcagaaaa agcttattat
     4861 gcttccatcg gaaacgatga tctggcagcc tgagttcaca gataaaacac tctccaggaa
     4921 acccggggcg gttcacttag ccgcgtgtcc attattgctg gataagatca taacgataca
     4981 ccgggttaaa caggtggtca ctgcttcact gaagctgcgt gattatgatg gtcaggtagc
     5041 agaagctatg cccatgatac caaaatgacg aaagccgaaa tgtctgaaag cgtgtgtatt
     5101 acctgaaaat atgaccggct ccggggtacg taaccgattc aacaaagcca atcgatatta
     5161 caaaacgaga gggactatat atttcgtttt cagataagac tccttgagag attatctttt
     5221 atgtaaaatc tcaaatatca cctgatgtaa tgtcaatgat aatattaagt gcgctctgaa
     5281 caacatcttt cttggtgtga ttaacatctg acaaaaagaa catcagcttc tttattatgt
     5341 tcttgttgtt tattatttca cgctca
//
LOCUS       DBANUU010000226         5237 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000226 DBANUU010000000
VERSION     DBANUU010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.230"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(99..659)
                     /locus_tag="ACIE68_004507"
     CDS             complement(99..659)
                     /locus_tag="ACIE68_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910696.1"
                     /translation="METQNQQTKPLNNIYAWILAFMPFFFGVSPEEYTNYAIIIGGVT
                     TIGLLVADRMALSESGCEPPSILWGVFLLPVYLWKRATITKGSRVPFIIIVASLAWVI
                     FIGIPHKDEVALEEAACPLVTTILKENNGSTAPKCMKVTIEDKVTDKFYRATATLDNG
                     NDINITLELTGDRNFYVRVPNVYLNN"
     gene            complement(821..1006)
                     /locus_tag="ACIE68_004508"
     CDS             complement(821..1006)
                     /locus_tag="ACIE68_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910697.1"
                     /translation="MLKEDSYLLKALEMRGVEPTVKNAHDIYLQFSPELKRFINQYGV
                     NDSSVKAMIDDELDHLI"
     gene            complement(1043..1342)
                     /locus_tag="ACIE68_004509"
     CDS             complement(1043..1342)
                     /locus_tag="ACIE68_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910698.1"
                     /translation="MLNITQAYQVALISSSHTSEEDARRFMIASNELDWVSQIEYGCI
                     VHAGLQDDDWKDELRRYGISEGAIANIQKVLDVGFDSVHFDCGAPVVGGLECWQW"
     gene            complement(1504..3537)
                     /gene="metG"
                     /locus_tag="ACIE68_004510"
     CDS             complement(1504..3537)
                     /gene="metG"
                     /locus_tag="ACIE68_004510"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFX5910699.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKAPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDTT
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3695..4795
                     /gene="apbC"
                     /locus_tag="ACIE68_004511"
     CDS             3695..4795
                     /gene="apbC"
                     /locus_tag="ACIE68_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFX5910700.1"
                     /translation="MSESKSPDALRAMVAGTLANFQHPTLKHNLTTLKALHHVALMDD
                     TLHVELIMPFVWNKPFEDLKEQCSGDLLRITGAKAIDWKLSYNIATLKRVKNQPGING
                     VKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQRPT
                     SPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDYLVL
                     DMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVENMS
                     VHICSNCGHHEPIFGTGGAQKLAEKYNTQLLGQMPLHISLREDLDNGTPTVISRPDSE
                     FTAIYRDLADRVAAQMYWQGEVIPGEIAFRAV"
     gene            complement(4833..5222)
                     /locus_tag="ACIE68_004512"
     CDS             complement(4833..5222)
                     /locus_tag="ACIE68_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFX5910701.1"
                     /translation="MGFPSPAADYAESRISLDQQIIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCALDGEYRVKRYRKYPRQHLEDLRTGKKEALPKDDDGCT
                     GSNAVFGVITHIINDARSGEFDDCPVM"
BASE COUNT         1347 a         1292 c         1249 g         1349 t
ORIGIN      
        1 ggatgtgatt gcgggtatgg aaggggagta gtggcgatca acggattctt atagaaaaga
       61 atcaaattgt gaccgccatt atcaatgagt tgcaaccgtt agttgttcag ataaacattt
      121 ggaacacgga cgtagaagtt cctgtcgccg gtaagttcca gagtaatgtt aatgtcatta
      181 ccattgtcta gtgtagccgt tgctctatag aacttatccg tcaccttgtc ctctattgtc
      241 accttcatac attttggtgc agtcgaaccg ttattctctt tcagaatagt tgtcacgaga
      301 gggcaggctg cttcttcaag agcaacctcg tccttgtgtg ggatgccgat gaagataacc
      361 caagccagcg acgcaacgat tatgataaat ggcactctgc tgccttttgt aatagtcgcg
      421 cgcttccaga gatagacagg caggaggaat accccccata gaattgaagg tggttcacag
      481 ccagactccg atagagccat tctgtcagct actaacaaac cgatggtagt tacaccccca
      541 attataatag cgtaattggt atactcttcc ggcgatactc caaagaagaa aggcatgaac
      601 gctagaatcc acgcataaat gttattcaga ggttttgttt gttggttctg ggtttccatc
      661 acatccattc catcacataa aaatcgcgtt tatatattat caattttctc acttgtataa
      721 cctaaatacg catccgttta tcaatccacc ttccacaaac atcagcttta gctatgaaaa
      781 ataacaatct caaagtgaaa accattatca taggaaacga ttagatgaga tggtctaatt
      841 catcatctat cattgcttta acacttgagt cattaacccc gtattgattt atgaaccgtt
      901 tcagctccgg tgaaaactga aggtagatat cgtgagcgtt tttcactgta ggttcaacgc
      961 cacgcatttc aagcgccttt agtagatatg agtcttcttt aagcataaat tctccgttgt
     1021 ctagtatgaa gcgattgccg gattaccatt gccagcactc tagccctcca acgaccggtg
     1081 caccacaatc aaaatgtaca gagtcaaagc ctacatcaag tactttctga atgttcgcaa
     1141 tcgcgccctc ggatatacca tatcgacgca actcatcttt ccagtcatcg tcctgtaacc
     1201 cggcgtggac aatgcaaccg tactcaattt gtgaaaccca atccagctca ttgcttgcga
     1261 tcatgaatcg gcgagcatct tcctctgatg tatgagatga gctgatcaat gccacctggt
     1321 aggcttgggt aatgttcaac atttcaacca ctccaaaaca agttgttttc ttatgtggtt
     1381 tattatctcc atcaacaaaa ggcagaaaac aaattgtttt aggtactgtg taaatcagaa
     1441 gggattgtgt ctatatgaaa atgcccggcg tgttgccggg cattgcgaag agaggttgtt
     1501 taattactta acctggtggc caggtttagc accagcatcc gggcttaaca ggaagatatc
     1561 tttcccgccg gggccagcag ccatcaccat gccttcagag ataccgaagc gcattttacg
     1621 tggtgccagg ttagccacca taatggtgtg acgaccaatc agtgcctgtg gatctggata
     1681 agcggaacgg atgcctgaga agacattacg cttttcacca ccgagatcca gcgtcaggcg
     1741 aagcagtttg tcagaacctt ctacaaactc tgcgttttca atcagcgcca cgcgcaggtc
     1801 aactttggcg aagtcgtcaa aggtgatagt ttcctgaatc gggtcgtcag ccagtgggcc
     1861 agttaccggt gcggctgtgg ctttcacttc ttctttggat gcttccacca gtgcttcaac
     1921 ctgcttcata tcgatgcggt tatacagtgc cttgaacgga ttcactttat ggcccagcag
     1981 cggttgctgg ataccatccc aggtcagttc tgtattcagg aatgcttctg cacgttcagt
     2041 cagtttcggc agtaccggct tcaggtacgt catcagcacg cggaacaggt taatgcccat
     2101 agagcaaatc gcctgcagat cggcatcgcg tccttcctgc ttcgccacca cccacggagc
     2161 ctgttcatcg acatagcggt tagccaggtc agccaatgcc atgatttcac ggatagcttt
     2221 accaaattcg cggctttccc atgcttcgcc aatcacttca gcggcatcag taaaggtttt
     2281 gtacagttgc gggtcagcca gttcgcttgc cagcacgccg tcaaaacgct tgttgataaa
     2341 gcctgcatta cgggaggcca ggttaaccac tttgttcacg atatcggcat tcacgcgctg
     2401 aacgaaatcc tccaggttga gatcgatatc atcaatgcgc gaagagagtt tcgcagtgta
     2461 gtagtaacgc aggctgtcgg cgtcaaaatg attcagccag gtgctggctt taataaaggt
     2521 gccgcgagac ttggacatct tcgcgccgtt caccgtcaca tagccgtgaa caaacaggtt
     2581 ggtcggcttg cggaagttgc tgccttccag catggcaggc cagaacaggc tgtggaagta
     2641 aacaatatct ttaccgatga agtggtacag ctcggcggtg gagtctttct tccagtattc
     2701 atcgaagctg gtggtatcgc cgcgcttgtc gcacagattc ttgaaggaac ccatgtagcc
     2761 aatcggcgcg tccagccaga cgtagaaata tttgcccggc gcgttcggaa tttcaaaacc
     2821 gaagtaaggc gcatctcggg agatatccca ctgttgcagg ccggattcaa accattcctg
     2881 cattttgttc gccacctgct cctgcaacgc gccgctgcgg gtccatgcct gcaacatttc
     2941 gctgaaagaa ggcagatcaa agaagaagtg ttcggaatca cgcattaccg gggtagcgcc
     3001 agaaaccacc gatttcggct cgatcaattc agtcgggcta tatgttgcgc cgcagacttc
     3061 acagttatcg ccgtattgat ctggcgcttt acatttcggg caggtgcctt ttacaaaacg
     3121 atccggcagg aacatgcctt tttccgggtc gtacaactga gagatagtgc ggtttttaat
     3181 aaaaccgttc tctttcaggc gagtatagat aagctcagac aactgacggt tctcttcgct
     3241 gtgcgtcgag tgatagttgt catagctgat gttaaagcct gcgaaatcag tctggtgttc
     3301 ctgactcatt tcgccaatca tctgctccgg tgtgatacca agctgctgtg ctttcagcat
     3361 gatcggcgta ccgtgggcat cgtcggcaca gatgaaatta acctcgtggc cgcgcattcg
     3421 ctggtaacga acccagacat cagcctggat gtgctccagc atatggccga ggtggattga
     3481 gccgttagcg tacggcagcg cgcacgtcac cagaattttc ttcgcgactt gggtcatagt
     3541 aggcattact tctttgtagt gaaaaggggc ttgatagtaa caaaatggcc ttatgtctgc
     3601 catgtgataa cagcatttct cataaatgat taaatgtcgt agctggagta cactacaagg
     3661 cgacaattgc gcaaattaaa ataaaggaga cgggatgagc gagtccaaat cgccggatgc
     3721 cctgagagca atggtagccg gtacgctggc taattttcag cacccaaccc tgaaacacaa
     3781 cctgactacg cttaaagcgt tacaccatgt tgcgttgatg gatgacacac tgcatgtcga
     3841 actaatcatg cctttcgtgt ggaataagcc ttttgaagac ctgaaagagc aatgtagtgg
     3901 tgatctgctc cgtatcactg gcgcaaaggc tattgactgg aagctgtcgt acaacattgc
     3961 cacgcttaag cgcgtcaaaa accaaccagg cattaatggc gttaagaaca ttatcgccgt
     4021 cagctcaggc aagggcggcg tgggtaaatc ctccacggcg gtaaacctgg cactggcgtt
     4081 ggctgctgaa ggtgcgaaag ttggtatttt ggatgccgat atctatggtc catcaattcc
     4141 aaccatgctg ggcgcggaaa accaacgtcc aacctcacct gacggtactc acatggcacc
     4201 tatcatgtct catggcctgg caaccaactc tattggttat ctggtcaccg acgacaatgc
     4261 aatggtgtgg cgtggaccga tggccagcaa ggcgctgatg cagatgttgc aggaaactct
     4321 atggcctgat ctggactatc tcgttctcga tatgccgccg ggcactggtg atattcagtt
     4381 gacgctggcg cagaacattc ctgtaactgg tgcggttgtg gtaactacgc cgcaagacat
     4441 cgcgctgatc gatgcgaaga aaggcattgt gatgttcgaa aaagtcgaag tgccggtact
     4501 gggtatcgtc gaaaacatga gtgtgcatat ttgcagtaac tgcggtcatc acgaaccaat
     4561 ttttggcacc ggtggcgcac agaaactggc cgagaaatac aacacgcagt tgttaggcca
     4621 aatgccactt catatctctc ttcgtgaaga tctggataac ggaacaccaa ccgttattag
     4681 tcgtccagat agcgaattta cggctattta tcgtgacctc gcagatcgcg ttgctgccca
     4741 aatgtattgg caaggtgaag taatacctgg cgaaatcgca ttccgcgcag tgtgataaaa
     4801 aggcggtgca ttgcaccgcc taatcagttc tcctacatca cgggacaatc atcaaactcg
     4861 ccacttcttg cgtcgttgat aatgtgagtg atcacaccaa acacggcatt gctgcccgtg
     4921 catccatcgt catcctttgg caatgcttcc ttcttaccgg ttcttaaatc ctccagatgc
     4981 tgacgtggat acttccggta tctttttacg cgatattccc catccagagc gcatacaagc
     5041 agagaaccat caaccggggt aagtgaggaa tcgaccacca gcaatgcacc ctgtaatatt
     5101 ccctcacgat gatgactatc agctgccctc atgaagtagg tcgctgaagg atgtctaatt
     5161 atctgctgat caagagaaat acggctctca gcataatccg ccgcaggaga agggaagccc
     5221 ataatactcg cctctta
//
LOCUS       DBANUU010000227         5065 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000227 DBANUU010000000
VERSION     DBANUU010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.231"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            1..939
                     /locus_tag="ACIE68_004513"
                     /pseudo
     CDS             1..939
                     /locus_tag="ACIE68_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
     gene            1148..2113
                     /gene="lipA"
                     /locus_tag="ACIE68_004514"
     CDS             1148..2113
                     /gene="lipA"
                     /locus_tag="ACIE68_004514"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFX5910702.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2214..2417)
                     /gene="tatE"
                     /locus_tag="ACIE68_004515"
     CDS             complement(2214..2417)
                     /gene="tatE"
                     /locus_tag="ACIE68_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFX5910703.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2546..3334)
                     /locus_tag="ACIE68_004516"
     CDS             complement(2546..3334)
                     /locus_tag="ACIE68_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFX5910704.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3427..3810
                     /gene="crcB"
                     /locus_tag="ACIE68_004517"
     CDS             3427..3810
                     /gene="crcB"
                     /locus_tag="ACIE68_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFX5910705.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLTANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3864..4073)
                     /gene="cspE"
                     /locus_tag="ACIE68_004518"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3864..4073)
                     /gene="cspE"
                     /locus_tag="ACIE68_004518"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFX5910706.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4248..4808)
                     /gene="pagP"
                     /locus_tag="ACIE68_004519"
     CDS             complement(4248..4808)
                     /gene="pagP"
                     /locus_tag="ACIE68_004519"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFX5910707.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1369 a         1265 c         1119 g         1312 t
ORIGIN      
        1 caaattgaac cctgtttaag cagaaagtcg tcagaaggta aaccacaaat atttacaacc
       61 ttacgaaata ttgatcttaa ccttctgact atttttgaag ctgtatatgt acataaaggg
      121 atcgttaatg cagcgaaagt gcttaatctg accccctcgg caatcagtca gtctattcag
      181 aaactgcgcg ttatattccc tgacccattg tttattcgca aaggccaggg tgtcactcct
      241 accgcatttg cgatgcatct acatgagtat atcagtcagg gccttgagtc cattcttggc
      301 gcgctggata tcgaaggaag ctatgataag caacgaacga taactattgc gaccactccc
      361 tcggtcggag ccctggtcct tcctgtcatc tatcgggcga ttaaaactca ctatccgtag
      421 cttttactgc gcaacccgcc catcagcgac gcggaaaacc aactcagtca gtttcaaacc
      481 gatctcatca tcgataacat gttttgcacc aatcgtacgg tgcaacatca tgttctgttc
      541 accgacaata tggtgttaat ttgccgcgag ggaaatccac tactctcttt agaagatgac
      601 agagagacta tcgacaacgc tgcgcatgta cttctgttac cggaagagca aaatttcagc
      661 ggtctgcggc agagagttca agaaatgttt ccggaccggc aaatcaattt caccagctac
      721 aacattttga caatcgctgc actggttgcc aacagtgaca tgttagcgat tattcccagc
      781 cgtttttata acctgtttag ccgctgctgg ccgctggaaa aactgccttt tccgtcctta
      841 aatgaggagc aaatagattt ctctatccac tacaacaaat tcagcctgcg tgatcccatc
      901 ctgcacggag taatagatgt tatccgtaat gcattttgaa aaagtattgt tacagccaga
      961 tcacagcgca gaacgataaa atggcacaat ccaacaacaa ttttacattt tcgcgaccgc
     1021 tttggctgct ttcaggtccg tttcaatgat atactgccag tcgttaattc aaaaatagtt
     1081 gataattaca acaatctatt gaattgaaac gctttccttc gtaattcgca actggaacac
     1141 gcacgctatg agtaaaccca ttgtgatgga acgcggtgtt aaataccgcg atgccgataa
     1201 gatggccctt atcccggtta aaaacgtggc aacagagcgc gaagccctgc tgcgcaagcc
     1261 ggaatggatg aaaatcaagc ttccagcgga ctctacacgt atccagggca tcaaagccgc
     1321 aatgcgcaaa aatggcctgc attctgtctg cgaggaagcc tcctgcccta acctggcgga
     1381 atgcttcaac cacggcacag caacgtttat gatcctcggc gctatttgta cccgccgttg
     1441 tccgttctgt gatgttgccc acggtcgccc ggtagctcct gatgccaatg aaccagtgaa
     1501 actggcgcag accattgccg atatggcgct gcgttatgtg gttatcacct ccgttgaccg
     1561 tgatgacctg cgcgatggcg gtgcccagca ctttgcggat tgcattactg ccattcggga
     1621 aaaaagcccg caaatcaaaa ttgaaactct ggtgccggat ttccgtggtc gtatggatcg
     1681 tgctctggat attctgactg caacgccacc agatgtgttc aaccataacc tggaaaacgt
     1741 accgcgtatt taccgtcagg tacggcctgg tgcagattac aactggtcgc tgaagctgct
     1801 ggaacgcttt aaagaagcgc atccggaaat cccgaccaag tctggtctga tggtgggact
     1861 gggtgaaacc aatgaagaaa ttattgaggt aatgcgcgac ctgcgccgtc atggtgtgac
     1921 gatgttaacg ctggggcaat atttgcagcc aagccgccat cacctgccgg ttcaacgtta
     1981 cgttagcccg gatgagttcg acgaaatgaa agccgaagcg ctggcgatgg gctttaccca
     2041 tgctgcatgc ggtccgtttg tccgctcttc ttaccacgcc gatttgcagg cgaaagggat
     2101 ggaagttaag taatcctgta ataattactt acatctgtct gataaaaaac ccgctttttg
     2161 aagcgggttt tttgtatcga acaagatatt gagggagcgt cctgctcgcc acgtcactct
     2221 ttatgagaga gcttttcagc ctgaagatca acgtctgcgc cttttttcgc cgcagcatcg
     2281 tcatcattca tcgccttctt gaacccttta atggccgctc caaggtctcc gcccagcgta
     2341 cgtaacttct tagtcccaaa cagcagaacg accagcgccg caactaccag cagtttggta
     2401 atactaatct cacccataga taccttcttg acatataacc ggcaacagaa cgcgccttaa
     2461 aaaccagaga atattaacgg tcattcgacg cgcgcacaca atagcaatct gtaacaaggt
     2521 gaatcaagcg ccgagtagaa aaacatcata ataattgcgg cggcgcaaag cgacggttgt
     2581 ttaagacggg cagttgcgcg cgcacctgac gcacacgttc gggcgtcact tccgccataa
     2641 tgagtgcagg catttctgac gctgccgcaa tggtgacgcc aaagggatct ataatccggc
     2701 tttgaccgat atttttgttc ccgcactccc ccgccgccac catataacag gtggtatcca
     2761 gcgcacgagc ggcaagcaac gttgaccagt gatgctcttt gagcggcccg cgaacccagg
     2821 cggcaggaag taccaggatt tcagctccct gtaatgcctg tgccagcgcc agctctggaa
     2881 agcgtaagtc ataacaggtc atcagaccga ccttcatccc ttccacctcc agtaacggag
     2941 cgatttcatt accagcatca acacggcgtg attcctgaat ggcaaatgca tcatagagat
     3001 gcagtttggc ataacgggcg acgatgtttc ctgcctgaag tgccaccagc atattccatg
     3061 cgcgccccgg cgttgaagga acatgaatcg tcagaattgt cgtcatcatg ttacgtttac
     3121 tttctcgccg taaacgtccg aggaattcgc cttccagcag ctgtgctgat ttaaccgata
     3181 gatctgcatc atgatcatcg cgcgccagca atgcttccgg caggacaaac agcgatacgt
     3241 cgttttccgc cgcctgcgcc atcaacgagg cacaaatctc agcgttcttt tcccacacag
     3301 atgtaacagc aaactgtcct gcggcaacca gcattgccac ctcctgtcag agtcgtaaaa
     3361 ttcgccaacg acgattattc ggcgttacac ttatcactca tacaaatcaa atagcaggat
     3421 tttgcagtgt tacaacttct tttagcagtt tttattggcg gtggtacggg aagcgtggcg
     3481 agatggctgt taagtatgcg atttaaccca ctgcatcagg cgattccgtt ggggacgctg
     3541 acagcaaacc tgattggggc attcatcata ggaatgggat tcgcatggtt cagcaggatg
     3601 acgaacattg atccagtgtg gaaagtatta atcaccaccg gattttgtgg cggtctaaca
     3661 accttctcaa cattttcggc agaagtggtg tttttgttac aagagggccg ctttggctgg
     3721 gcattactga acgttttcgt caaccttctg gggtcttttg ccatgaccgc actggcattc
     3781 tggctgtttt cggcctcaac cgcacactaa agaccaaaaa aaacccgctg attaagcggg
     3841 ttttgaattc ttgctgacgt atcttacaga gcgattacgt ttgcagcaga agggcctttg
     3901 gcaccgttag tgatttcgaa ctctacgcgc tgaccttcag caagagtttt aaaaccatta
     3961 gtctggattg cagagaagtg tacgaacacg tctttgctgc cgtcttccgg agtaatgaaa
     4021 ccgaatcctt tggactcatt aaaccactta acgttacctt taatcttaga catcaaaatt
     4081 acctttacat gaaaaataga cacaaatgct gtgtcggtta ccagtacacc aattgtggta
     4141 cgctttgtcc agtgcaactt tactaaaaag tgataaaagt cgcgttattt ttgatgttac
     4201 ttattttagc tattgatttt aaagaagtta ctaaaacttc atttgcctca aaactgaaag
     4261 cgcatccagg caaagtacac attgccattg ttgtaggtac ccggaatgta ggtcatctga
     4321 aaagtcactg ggccataacc cacggaggcc aatggcagta gaaccgggag agggatgtaa
     4381 ttccagttat cgcgtgccgt tacgccagcg gtgaatccca gacctaaatg aaaattttca
     4441 tccgccagcg gtcgccaggt actttcccat ccgtatccgg caatcggttc ccatttgttc
     4501 cacgagtcct taaatgccat ggcatacagg ccatgccagt ttcctttttc atcccaacgc
     4561 gacaggccaa aaccgccacc ccacggtcgc tcgttatagc gatcggtttt ttctttgtcg
     4621 taagcgaaac gtgcatgcca ggtgatggca ggaatatata aatcataatg ttcaggctgt
     4681 tgccaggttt gtgcaatatt ttctctaaac gttgtcatcc actcatctgc gttagcaaaa
     4741 actttaccaa cgctgattaa ctgaataaaa acaaaggaaa agatagcgac atatttactc
     4801 acgttcattt gtgaccataa aacatttatc aaaaatctac tactagcata gcaaagctac
     4861 aattaacata accttaatag acccaacata aagaataatc tgaatagctg ctctatttat
     4921 ttccgcttgt tcaagttaaa tatttccgcg catcgattaa agatgatgcg tcgttttctg
     4981 cgatgggaat agtcaaaaaa gaaaaaccaa gtcttttttg atgacaaaat gcaatcaagg
     5041 aaaaattatt ttatttttta acgag
//
LOCUS       DBANUU010000228         4988 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000228 DBANUU010000000
VERSION     DBANUU010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.232"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..427
                     /gene="traV"
                     /locus_tag="ACIE68_004520"
     CDS             <1..427
                     /gene="traV"
                     /locus_tag="ACIE68_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFX5910708.1"
                     /translation="TTSDTCMTMEQANEKAKKLERSSEAKPVAASLPRLAEGNFRTMP
                     VQTVTATTPSGSRPAVTAHPEQKLLAPRPLFTAAREVKTVVPVSSVTPVTPPRPLRTG
                     EQTAALWIAPYIDNQDVYHQPSSVFFVIKPSAWGKPRIN"
     gene            562..783
                     /gene="traR"
                     /locus_tag="ACIE68_004521"
     CDS             562..783
                     /gene="traR"
                     /locus_tag="ACIE68_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFX5910709.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            776..1252
                     /locus_tag="ACIE68_004522"
     CDS             776..1252
                     /locus_tag="ACIE68_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910710.1"
                     /translation="MHKSENEKIDFILDEHERIFRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFTGGNKSE"
     gene            1332..1549
                     /locus_tag="ACIE68_004523"
                     /pseudo
     CDS             1332..1549
                     /locus_tag="ACIE68_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1577..1924
                     /locus_tag="ACIE68_004524"
     CDS             1577..1924
                     /locus_tag="ACIE68_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910711.1"
                     /translation="MKPIYLKYFLSFVFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTIGKANKIASECRKRNEMAEARRVREYERNKIREKQ
                     LQALNDAHSVKER"
     gene            2051..4681
                     /gene="traC"
                     /locus_tag="ACIE68_004525"
     CDS             2051..4681
                     /gene="traC"
                     /locus_tag="ACIE68_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFX5910712.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGVPFCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAATQFPLPEGMNLPLTLRHYR
                     VFFSYCSPSKKKSRADILEMENLVKIIRASLQGASITTQAVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKKNKARIDDVVDFLKNARDNDQYVESPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            4678..>4988
                     /gene="trbI"
                     /locus_tag="ACIE68_004526"
     CDS             4678..>4988
                     /gene="trbI"
                     /locus_tag="ACIE68_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFX5910713.1"
                     /translation="MSSTQKPADVTAERRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTVAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PA"
BASE COUNT         1282 a         1177 c         1350 g         1179 t
ORIGIN      
        1 caccacatcc gatacctgta tgacgatgga gcaggccaat gagaaggcca aaaaactgga
       61 gcggtcctca gaagcaaagc cggttgcggc atcactgccg cgcctggctg aagggaactt
      121 ccggacaatg ccggtgcaga ccgtcaccgc gaccactccg tccggcagca gacctgcggt
      181 gacagcacat ccggagcaga aactgctggc acctcgcccg ctgtttaccg ctgcccggga
      241 agtgaaaacg gttgttccgg tcagttcagt tacgccggta acacctcctc gtccgctaag
      301 aacgggtgag cagacggcag cattatggat agcgccttat attgataacc aggatgttta
      361 tcatcagcca tccagcgtgt tttttgttat taaaccgtct gcgtggggaa aaccacgtat
      421 taattaactg gccctgaagt tgtatatcag ccggcaatgc cggcgggctt tctgtattca
      481 aaatctgcct gtaaaacgag tgccctgtgc gtgaaaaggg atgggcggta gcgtgtcaga
      541 acatgtcggg aggaatattc cgtgagtgat gaagccgatg aagcatattc agtgacagaa
      601 caactgacca tgacaggaat aaaccggata cgccagaaaa taaatgttca tggtattcct
      661 gtttatcttt gtgaagcatg cggaaatcct attccggaag cccggcggaa aatatttccc
      721 ggtgtgacgt tgtgcgttga atgtcaggcg tatcaggaaa ggcagaggaa acactatgca
      781 taaatcagaa aatgaaaaaa tagattttat tctggatgag catgagcgga tattcagaaa
      841 acagggttct ttgtgtatgg cactgggtaa gggcttttta gttatagcta ttctttgtgg
      901 tgttgccaca ttctgtgttt ctggcctgta tctgaaatcc ctgtgcctgt ctgttgcctg
      961 ctgttgtggg gtattcagtc gtatttttaa ttattacagc gtaccggagg aatcacgcat
     1021 ggtactcagt cgtcaggaat tactccggct tatgcgtctg actgaagacg taccagatgt
     1081 gagacaggaa ctgcttaacc gtttgttgtc cggtaaaaaa ctgactggtc gggatgaaaa
     1141 ggatatccgg cgtctctggc aggagaaagt ggatgcaatg caggagtctg ccacccgtca
     1201 gcgtgaacag gatactattc ggaagtttac tggagggaat aaatccgaat gagaatctgc
     1261 agtgttttat gacctcataa gttaactgaa gggatattca gtgattaata tacaagcgga
     1321 ggtaataaag aatgcatacc tttatttttc ttgtgctgat aaaaatatta acggtggcgg
     1381 aagctggaat attacaccca tgccaaatac ggatgtatgc gtgcaggttc ttcggacaat
     1441 cgaagcgcac ggaggcaggg gcttaggaaa tacaggggag tgtattgaag ttaaaacaaa
     1501 tgaacctttg aacaacgtga tggaaacact gaatggtggt gggcaatgag tgcttactgc
     1561 ttcgaaggaa aatgaaatga agccgatata tttaaaatat tttctgtctt ttgtatttgt
     1621 tggtgttatg gctatgattg tatgtattcc tttttacatc gtttaccttg cgcaacaacc
     1681 tgcaacacca gaacaactga cagagatttt acaggaaact ccctgtgcag cagaagcatt
     1741 tcaggagaca cttaattatc agtctgaacc tttaaccatt ggtaaggcaa ataaaatcgc
     1801 atcagaatgc agaaaaagaa atgaaatggc agaggcgcga cgggtaagag aatatgagcg
     1861 caataaaata cgcgaaaaac aacttcaggc attaaatgat gctcattcag taaaggaacg
     1921 ttaatcctgg tttcctgtta aaagcctcca tttcttattt tgtgctgtgg ttttaagaat
     1981 ggcactgcgt gatatatcca cggccacaga ctgatatttt ccttttttaa acgtactaag
     2041 gaatactcat atgagtaata acccacttga ggccgtcact caggcggtga actcccttgt
     2101 caccgcactg aaactgcctg acgaatccgc aaaggccaat gaagttctgg gcgaaatgag
     2161 cttcccgcag ttcagtcgtc tgctgccgta ccgtgattac aaccaggaat ccggtctgtt
     2221 catgaatgac accacgatgg gctttatgct ggaagccatt cccatcaatg gggcgaatga
     2281 gtccattgtg gaggccctcg atcacatgct gcgcaccaaa ctgccgcgcg gggtgccgtt
     2341 ctgcatccat ctgatgtcca gtcagctggt tggtgacagg attgaatacg ggctgcgtga
     2401 gttctcctgg tcgggtgaac aggctgaacg gtttaacgcc attacccgtg cctattatat
     2461 gaacgcggca gcgacacagt ttccgctgcc ggaggggatg aatctgcccc tgaccctgcg
     2521 ccattaccgg gtattcttct cgtactgttc tccctcgaag aaaaaaagcc gggctgatat
     2581 tctggaaatg gaaaacctgg tgaaaatcat ccgggcgtcg ttacaggggg ccagtatcac
     2641 cacacaggcg gtggatgcac aggcctttat cgatattgtc ggggagatga ttaaccataa
     2701 cccggactcc ctgtatccga aaagacgtca gctggacccg tattctgatc tgaattatca
     2761 gtgtgtggag gacagttttg acctgaaagt ccgggcggat tacctgacgc tgggcctgcg
     2821 tgagaacggc aggaacagca cggcccgcat cctgaatttc catctggccc gtaacccgga
     2881 aatcgccttc ctgtggaaca tggccgacaa ctacagcaac ctgctgaacc cggaactgtc
     2941 catctcctgt ccgttcatcc tgacgctgac tctggtggtg gaagaccagg tgaaaaccca
     3001 cagcgaagcc aacctgaagt acatggacct ggagaaaaag tcgaagacct cctatgccaa
     3061 atggtttccg tccgtggaga aagaggcgaa ggagtggggg gaactgcgtc agcggctggg
     3121 ctccggtcag tcttctgtcg tgtcctactt cctcaacatc acagcattct gcaaggacaa
     3181 caatgaaacg gcgctggaag tggagcagga cattctgaac agctttcgta aaaacggttt
     3241 tgagctgatt tcaccgcgct ttaaccacat gcgcaatttc ctgacctgtc tgccctttat
     3301 ggccgggaaa gggctgttta aacagctgaa agaggcaggt gtggttcagc gcgcagagag
     3361 ctttaatgtt gccaacctga tgccgttagt ggcggataac cccctgacac cggcaggtct
     3421 gctggcaccc acctaccgta accagctggc gtttatcgat attttcttcc gggggatgaa
     3481 taacaccaac tacaacatgg cggtctgtgg cacctccggg gccggtaaaa ccgggctgat
     3541 acagccactt atccgcagcg tgctggactc cgggggcttt gccgtggtgt tcgacatggg
     3601 ggatggctat aagtccctgt gtgagaacat gggtggggtg tatctggacg gtgaaaccct
     3661 gcgttttaac ccgtttgcga acatcaccga tattgaccag tcagcggagc gtgtccgtga
     3721 ccagttgtcg gtgatggcca gccccaacgg taacctggat gaagtacatg aaggtctgct
     3781 gctgcaggcg gtcagggcct cctggctggc taaaaagaat aaggcgcgca tcgatgatgt
     3841 ggtggatttc ctgaaaaacg cccgtgataa cgaccagtat gtcgagtcac cgaccatccg
     3901 cagccgtctg gacgaaatga ttgtgctgct tgaccagtac actgccaacg gcacttacgg
     3961 ccagtatttt aactctgatg agccgtccct gcgggatgac gcaaaaatgg tggtgctgga
     4021 gctgggcgga ctggaagacc gtccgtcact gctggttgcg gtgatgttca gccttatcat
     4081 ttacatcgag aacaggatgt accgcacgcc gcgtaatctc aaaaaactga acgttattga
     4141 tgaaggctgg cgtctgctgg acttcaaaaa tcacaaggtc ggtgaattta ttgagaaagg
     4201 ttaccgtacc gcccgccgtc ataccggtgc ctatatcacc atcacacaga acatcgtcga
     4261 ctttgactct gacaaggcct ccagtgctgc acgcgcggca tggggtaact cctcctacaa
     4321 aattatcctc aaacagagtg cgaaggagtt cgcgaaatac aaccagctgt atccggacca
     4381 gttcctgcca ctgcagcgtg acatgattgg taagtttggt gcggccaaag accagtggtt
     4441 cagttccttc ctgctgcagg tggaaaacca ttcctcctgg catcgtctgt ttgtggaccc
     4501 gttaagccgc gccatgtaca gctctgacgg cccggatttt gagtttgtgc agcagaaacg
     4561 taaagagggg ctgagtattc atgaggcggt gtggcagctg gcgtggaaga agtcagggcc
     4621 ggagatggct tcgctggaag cctggctgga agaacatgag aaatacagga gtgtggcatg
     4681 agttcaacgc agaaacccgc cgacgtcacg gcagaacgac gcagccactg gtggtggacg
     4741 gtgccgggat gcctggcaat ggtgttactg aacgcagccg tcagttacgg cattgtcaga
     4801 ctgaacgcac cggtgactgt cgccttcaac atgaaacaga ccgtggatgc gttttttgac
     4861 agcgccagtc agaaacaact gtcagaagcg cagtcaaaag ccctttcagc gcgttttaac
     4921 acggcactgg aagccagcct gcaggcgtgg cagcagaaac accacgcggt cattctggtg
     4981 tcgcctgc
//
LOCUS       DBANUU010000229         4901 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000229 DBANUU010000000
VERSION     DBANUU010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.233"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(53..814)
                     /gene="kduD"
                     /locus_tag="ACIE68_004527"
     CDS             complement(53..814)
                     /gene="kduD"
                     /locus_tag="ACIE68_004527"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFX5910714.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(844..1680)
                     /gene="kduI"
                     /locus_tag="ACIE68_004528"
     CDS             complement(844..1680)
                     /gene="kduI"
                     /locus_tag="ACIE68_004528"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFX5910715.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1967..3148)
                     /locus_tag="ACIE68_004529"
     CDS             complement(1967..3148)
                     /locus_tag="ACIE68_004529"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFX5910716.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3403..4632
                     /locus_tag="ACIE68_004530"
     CDS             3403..4632
                     /locus_tag="ACIE68_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFX5910717.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1286 a         1245 c         1067 g         1303 t
ORIGIN      
        1 agatataaaa aaccctgcca tgcggcaggg tcataaaagt aagaagaatg aattaacgcg
       61 ccagccaacc gccatccacg gcaatggtat aaccattcac ataatctgaa gcgctggagg
      121 caaggaacac taccggcccc atcaggtcac tcggcagtcc ccaacggcca gctggaatgc
      181 ggtcgaggat ttccgcgcta cgttgttcat ctgcccgtag ttgttgagta ttgttggtcg
      241 ccatgtaacc cggggctatc gcattaacat taatgttgtg tttagcccat tcgttcgcca
      301 tcaatcgcgt cacacccatc acgccgcttt ttgatgcggt ataagaaggc acacggatcc
      361 cgccctggaa ggagagcatt gacgcgatat tgataatctt gccgccattg ccttgcgcga
      421 taaagtgttt cgccgctgcc tgagacatga agaatacgct cttgatattc aggttcatga
      481 catcgtccca gtctttttcg ctgaactcga gagcgtcttc gcggcgaatc aatccggcgt
      541 tattcaccag gatatcaata tgaccaaatt cagctaccgc gcgatccagc agtccaggaa
      601 tgccatcaat ctttcgcaga tcggcggtca ggcttaaaaa acgacgcccc agcgccgtga
      661 cctgctcgat ggtttcagtc ggttcaacga tgttaatgcc aacaatgtca cagcccgctt
      721 gcgccagccc caacgccatc ccctggccca gcccagtatc acaaccagtg acgaccgcaa
      781 ctttaccttc gagagaaaat gcacttaaaa tcataacaat accttattcg tttatgccca
      841 caactagcgc aaatctttaa cggccacatg gtccatatca tcaaagacct ggttttcacc
      901 aaccatgccc cagataaagg tataagcttt ggttccgacg ccggaatgga tcgaccagct
      961 cggggagatc accgcctgct cgttatgcat cacaatgtga cgcgtttctt gcggttgccc
     1021 catcatgtgg aaaacgcagg cgtcatcatc catattgaaa tagaaataaa cttccatccg
     1081 gcgctcgtgg gtgtgacacg gcatggtgtt ccacaagtta cctggagcca gctccgtcag
     1141 ccccatactc aattggcagg tttccagcac atccgggaca aaatatttgt taatagtgcg
     1201 acggttactg gtgaggttat cgcctaacgt gactggagat acttcgtccg gggtgacttt
     1261 tttggtggga tacgtcgtat gcgcgggtgc gcaattgtaa taaaacttcg ccggagtgcc
     1321 ggtatcaata ctggcaaaga caacttcttt tgcaccttta ccaacataca gggcgtcgcg
     1381 gtgaccgatt tcatagcatt ggccatcgac agtaatcgta ccggcaccgc caatattgat
     1441 aacacctaac tcgcgacgtt caaggaaata gcttacgccg agttgtttac caacttcccc
     1501 gccaacggaa accgttttag ttatcggcat aatgccgcca acaataattc ggtcaatgtg
     1561 gctgtaaacc atggtgtact catcggcgac aaataccttt tcaaccaaaa attcattgcg
     1621 cagcccttgg gtatccagcg tttttgcgtg cgcactgtgg atgctctgtc ttacgtccac
     1681 atcaacctcc gataatcagt gaagcgtgaa aatagaaaac gatagtgaaa cactgtttcg
     1741 ttttctctat aaagggatga tatgcccatg ggctagggtt ttcaattaaa aataaaacaa
     1801 tgtttcattt ttaagttaag gattaaaaaa agtgcagtgt cgatcacgaa tatgtcatga
     1861 gaatggaaaa atgagggagc ccagaaaatg tatgtcgact tgccagccat cattattcaa
     1921 cagattaatg acggcagata tttatcggta aggaggaaat gcagagctat tcgtcacgtt
     1981 caatggtcaa tgccacaccc tggcccccgc cgatacaaag cgttgccagt cctttgcggg
     2041 cattacgttt caccatttca tgaaccagag aaaccaggat tcggcaaccg gaagcgccta
     2101 tcgggtgacc aagtgcgatc gcaccaccat tgacattgac ccgacgctca tcccactcaa
     2161 gcatcttgcc aaccgaaagc gcctgtgcag caaacgcttc attagcctcg ataagatcga
     2221 catcagccaa ctgccagcct acacgctcca ggcaacggcg ggtcgcatac accggcgcaa
     2281 ttcccatcaa tgccggatct acaccaacgc tggcaaatgc gcgaatgcgg gccagcacgg
     2341 gtaaattcaa cgctcgtgct ttggcttcgc tcatcatcat taccgcagct gcgccatcgt
     2401 ttatggatga tgcattaccc gctgtcacag aaccgagact atcaaatgaa ggatttaaac
     2461 gggctaaacc ttctgcactg gcgtcagtgc gtggctgttc atcggtatca acaaccaacg
     2521 tctgaccgtt actttgggtc attaccggga cgatctcatc tttaaatcgt ccggcgtcaa
     2581 tcgccgctcg cgctttttgt tgcgagctaa gtgcgtaagc atcctgcaat tgacggctga
     2641 tgccatattc gcgagccaga ttttcggcgg tgacaccaat atgataatca ttgaaggcat
     2701 cccataaccc atcatgcaca agactgtcaa ccagctggct attgccaagc tgtgcaccgg
     2761 tgcggctatc agtcagaaca tgtggtgcgc ggctcatgtt ttcctggcca ccggcgatga
     2821 caatatcagc ctcgccacac tgtatcgcct gagtagccag atgcagtgct ttaagtccgg
     2881 aaccgcagac gtcattaatc gtgattgcag aaacgctatt aggcagacca cctttaatag
     2941 ccgattgcct tgccggattc tgccctgccc ctgcagtcaa cacctgacca agaattactt
     3001 catccaccgc atatgcagga acgccggtac gttctattaa cgctttcacg accagactac
     3061 caagttccac ggcggaatga cccgctaacg caccgcgaaa gcagccgata ggtgtccgta
     3121 acgccccgac aatcacaacg tctttcatca caacctcgca ccaattaaca gtgcaatagt
     3181 aaatgattgt taattacagt tatcttaatt gtttaaaaaa agtgattttt atcacaaagg
     3241 aaatatgcct gagcagtagt cagagtcata actggcacgt aaggtttgca accactaacc
     3301 caccaataga ggggtagata gggcgttaat ctcccatact taacctggtt tatggtaaat
     3361 tgccctccat tttgtttaat ttgtagatga tacgttcaga taatgtctaa tatttggtct
     3421 aaagaagaaa ctctgtggag tttcgcgctc tacggcacag ccgttggtgc aggcacgctc
     3481 ttccttccta ttcagttagg ttcggcaggg gctgtggtcc tgtttattac tgctctggtc
     3541 gcctggcctt taacatattg gccacataaa gccttatgcc agttcatcct ctcatcgaaa
     3601 acatcagcag gtgaagggat aacgggcgca gtaacacact actatggcaa gaagattggt
     3661 aatctgatta ccacgctgta cttcatcgcc ttttttgtcg tcgtgttgat atatgcagtg
     3721 gcaattacca actcacttac ggaacagctg gcaaagcata tggttattga tcttcgcatc
     3781 cgtatgttgg tgagtctggg tgttgtatta atcctgaatc tcatttttct gatgggacgc
     3841 catgcaacca ttcgggtaat gggttttttg gtattcccat tgattgccta tttcttattt
     3901 ctttccattt accttgtcgg tagttggcaa cctgatctat taacgaccca ggtagagttc
     3961 aatcagaata cccttcacca gatatggata tcgattcccg tgatggtttt cgcctttagc
     4021 catacgccca ttatttctac gtttgccata gacagacgtg aaaaatatgg cgaacacgct
     4081 atggataaat gcaaaaaaat tatgaaagtc gcttatctca tcatctgcat aagtgtactg
     4141 ttctttgtct ttagctgcct gctttctatt ccaccttcgt atattgaagc ggctaaagaa
     4201 gaaggggtca ccattttatc ggcgctttct atgctgccga acgccccagc atggctgtca
     4261 atttccggga ttattgtcgc agtagttgcg atgtcgaaat cattcctggg tacgtatttt
     4321 ggcgttattg aaggtgccac ggaggtcgtc aaaacaacac tacagcaggt tggtgtaaag
     4381 aaaagtcgtg catttaaccg cgcactatca attatgttgg tatcgctgat caccttcatt
     4441 gtttgttgca ttaacccgaa cgcgatttcg atgatttacg cgatcagcgg cccgctcatt
     4501 gccatgatac ttttcatcat gcctacgttg tcaacgtatc tgatcccggc gcttaaaccc
     4561 tggcgttcca tcggaaatct gattacgctg atcgtgggta tcctgtgcgt atcggtaatg
     4621 ttctttagct gatatccatt gtgaatggtt caaaattgct acaattttga accattccag
     4681 ttttcataat gttcattcgt tattattccg cactatcact tccctcatca tatcctagta
     4741 ttatcagtta gttaaacaca caaagagatc tgaacatctt aaataagact atttaagatg
     4801 cataacttag attcgcaaga tatcttccgg tcattcaaaa acaatttgcg atatagatat
     4861 tctcatacca tgagatagtt atctgaacca ccctcaacca g
//
LOCUS       DBANUU010000230         4890 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000230 DBANUU010000000
VERSION     DBANUU010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.234"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..190)
                     /locus_tag="ACIE68_004531"
     CDS             complement(<1..190)
                     /locus_tag="ACIE68_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910718.1"
                     /translation="MMKAHVFVISMLTGIVVTYAVLLLGCLFIDKTLPTVDVVILSLV
                     VGASAQQLSRVLMSINRTF"
     gene            complement(241..654)
                     /locus_tag="ACIE68_004532"
                     /pseudo
     CDS             complement(241..654)
                     /locus_tag="ACIE68_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
     gene            652..909
                     /locus_tag="ACIE68_004533"
                     /pseudo
     CDS             652..909
                     /locus_tag="ACIE68_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(1143..2000)
                     /gene="sitD"
                     /locus_tag="ACIE68_004534"
     CDS             complement(1143..2000)
                     /gene="sitD"
                     /locus_tag="ACIE68_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFX5910719.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKSWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQASGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISILVSMTGVWLSFFIDSAPAPTIVVLFAVMFIMTFTVTSINARTKENA
                     EPRDLLSSD"
     gene            complement(1997..2854)
                     /gene="sitC"
                     /locus_tag="ACIE68_004535"
     CDS             complement(1997..2854)
                     /gene="sitC"
                     /locus_tag="ACIE68_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFX5910720.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQLTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2851..3678)
                     /gene="sitB"
                     /locus_tag="ACIE68_004536"
     CDS             complement(2851..3678)
                     /gene="sitB"
                     /locus_tag="ACIE68_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFX5910721.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPGGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(3678..4592)
                     /gene="sitA"
                     /locus_tag="ACIE68_004537"
     CDS             complement(3678..4592)
                     /gene="sitA"
                     /locus_tag="ACIE68_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFX5910722.1"
                     /translation="MLSIKKVTMLLGCLVLTCSIAFQASAAEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDAFIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQIAELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1348 a         1229 c         1144 g         1169 t
ORIGIN      
        1 ggaaggttct atttattgac attaataccc tcgacaattg ctgagcagaa gcccccacga
       61 caagtgacag aatgacaaca tctacagtgg ggagcgtttt atcgatgaac aagcaaccaa
      121 gaagtaacac ggcataggtg acgacaatgc cagtaagcat agaaataaca aaaacgtgag
      181 ctttcatcat tcctccagaa taaccccgac gtgtaagcct gggggattgg cacaataatg
      241 tcatttaaag gcagactttc cggcaaattg cgccgatgaa cccagttcat cctcaattct
      301 cataagctgg ttatattttt caatacgctc accccggcat ggagctcccg ttttgagatg
      361 acctgcgcgc attgcaactg tcatatcggc gataaagctg tccactgtct caccactgcg
      421 gtgtgagata aaggtgcccc agttgttgtc atggcaaagt tgtaccgcat caaaggtttc
      481 actcagggaa ccaatctggt tgagtttaat caatgctgaa ttggcaaggt tttcgtcaat
      541 gccgcgctga atatacttta cgttggtcac aaacagatcg tcgccaacca attctatctg
      601 ctctcccaat gcaccatgca ggattttcca tccagcccag tcatcttcag ctaccgttaa
      661 gggcaggaag atcaactagg tgctctgggg ctggtgacca atgcggttgt gctgtgaaac
      721 acactttaca tgcaggaggc gttgtcacat cttcgcaaat cgggagaaac tcctgaggag
      781 tagcatccgg caagattatc gctgctgata catagccata taaatatgct ggggcattat
      841 acgtcttcgt taccggaaga tattcttaag ggtgagttaa gatcattaaa cttcaataca
      901 aacaattaat taaactctta gcgtggtttt ttacatcact ggccctcgcc ccccaatttt
      961 ctctatgttt tgccactact tccctcattt tactgcgctg tttgctaccc ctgagaatcg
     1021 acattacttg ctatccagtc cgcagcaact ctctggcaac aaaacttatc agaatcataa
     1081 gtctgtgcat tattcgttca agcgcatcgc caataagcgg atgttgctgg ccgagtgttc
     1141 tgctaatcgg atgataacaa atcccgtggt tcggcatttt cctttgtgcg tgcgttgatg
     1201 ctggtaacgg taaacgtcat gatgaacatg accgcgaata agacaacaat tgtcggagct
     1261 ggcgcgctgt cgataaaaaa ggacagccag acaccagtca ttgagaccaa tatcgatatg
     1321 ccagtcgcca gcaggagcgc aatatgaaag cgttgtgtca ggagtaccgc aatggcacca
     1381 ggcgcaatga gaagagaaat agagagaatg atacctaccg ctttcagcgt cgccacaatg
     1441 gtcagggaga ccatgcacag cagcccataa tgcagccatc tcgtatgcaa accgcttgcc
     1501 tgcgcctgtt gataatcaaa gctgaacagc aaaaaatccc gccattttac gctaataaca
     1561 agtgtaacca gtccggcaat aatcaccgtc tggattatat cgccgatggt tatcccgagc
     1621 atgtcgccga aaagaatatg atcaagatgt acgtctggtt tgactgcaat atacaagatc
     1681 agacctgcgg caaacatgcc agaaaaaaca atccccatca ctgtatcttg cttgattcgg
     1741 ctgttgtctt tcaggtatcc cgtcgccacc gcacagaata ctccggcgac gaatgcccct
     1801 gtagccagag gtaaccccag aatccaggca agcacgatgc caggaaaaac cgcatgactc
     1861 atcgcgtcac ccatcagtgc ccaacttttc aggaccagaa agaccgacag cagcgcacag
     1921 ggtatggaaa ctaccagtga aatcagcagt gcgtgactca taaaggtgaa ctgcagtggc
     1981 tccagtaata atgccatcat gagcgatcct ccagcgcttt atgcgcacgc cgacgatttg
     2041 ccaaaagccc atgggtgggc gcaaagacaa atgctaaaag aaacagcagt gtctgcgcca
     2101 ccacgatgat tccacctgtc gcgccgtcca gaaagtaact gacccacgcg ccgaggaaac
     2161 tggtcacact gccaatagtt acagcaatca tgagcaggcg gggaaaacga tcggttaaca
     2221 accatgctgt tgcaccggga gtcaccacca gacagatcac cagaaaggca ccaaccgttt
     2281 gcagcgccgc aacggtcgaa accgacagca gggtgaagaa gaggattttt aatctgcccg
     2341 gatgcaatcc aatagcacgg gcgtgattct cgtcgaaaaa ggtcaccata agatctttcc
     2401 atttgaaaaa aagcacgatg atcgagagaa tgccaatgat agtgagttgc aggatatccg
     2461 ccggatcgat tgccagaata ttgcccagga cgatggtctg aatgttcacg gatgtcgggt
     2521 tcaatgacac cataaacagc cccaggccaa aaaaggacga gaagatcagg ccgatgatgg
     2581 catcttcttt taagcgagtg agttggttaa gaaacagcat gctgcccgct gccagtccgc
     2641 cggaaaagaa ggctcccaga gaaaacggaa gccccagcat atatgccccc gcaacacccg
     2701 ggacaataga gtgcgacagc gcatcgccaa tcagcgacca gccttttagc atcagatagc
     2761 atgacaggaa agcgcagaga ccgccgacca tcgccgagac ccacatcgca ttgagcatat
     2821 attcatagct gaagggttcc agtagcacgt tcatctttct tccctctgca ccgctgacgg
     2881 tcgatgtgcc acaaaagggc gttcatcgtc agtaataatg ctttcttccg agccgttaag
     2941 tgtgacatgg cgcagtacgc cgctaaaagc cagctccagg ttctcggctg taaaagtggt
     3001 gtctgtcggc ccgcttgcca ggacggtgcc tttgaccatg accgtataat cgcaaaatgt
     3061 cgtgacggat ccgagattgt gcgttgagac aagcatcgtt ttgccttccg cgcgtaactc
     3121 cctcaacagg ctgatgattt tcgcttcggt tttcacatca acgccggtaa aaggttcatc
     3181 cagcaggatc acatcaccct gctgtgcaat cgctctcgcc agaaagacgc gtttcttttg
     3241 tccaccggaa agctcgccga tttgtcgatg gcgaaaatcc accatatcga cacgttccag
     3301 cgcatcggtg acaatctgac gatccctctt tttggcgata cgcaacatac ccatatgccc
     3361 atagcggccc atcatcacca catcttccac cagtacagga aatgaccagt caacctcttc
     3421 tgactgaggg acgtaggcaa ccaggttttt ctgtagcgcc tgtcgtgtgg gaatacccag
     3481 aacagatatc ttcccgctgg tcagacgcac aaatcccata atcgctttaa acaacgtcga
     3541 ttttccggaa ccgtttaccc caaccagagc ggcaatagat ccgcctggta ctgtgaagga
     3601 tgcatcgcgc agtgctgtgt gcccgttacg ccaggtgaca gtaacatcat taacgacaat
     3661 gcctgcagat tgcatcatta tttctccctt tttccggctt taattccctg taccagcgta
     3721 ctggtagtaa ccttcagaag gtcgatatac gtaggtaccg ggccgttttc tgtgctcagg
     3781 gaatcgacat agagcacacc accgtagtgc gcgccggttt cacgcgcaac ctgacgcgct
     3841 ggtttatcgg aaatcgtact ctcgctaaag actgccggga tatgattttt cttaactata
     3901 tcaacaacct tacgtacctg ctgcggtgtt ccttgttgat cggcattaat cggccacaga
     3961 taaagctctt ttagccccaa atcgcgtgcg agataagaaa aagccccttc actggtgacc
     4021 atccatcgct gattctcagg gagttccgca atctgcttac gcaggggggc aagggtttgg
     4081 gtaatcttgg ctttataagt atcggcatta cgttggtagg tttgtgcatt tgccggatcg
     4141 tattttataa acgcatcacg aatattatcg acgtaaatca gagcattatc tggcgacatc
     4201 caggcatggg ggttaggttt gccctcatag ggcccttcgg tgatccctac tggcgtcaca
     4261 cccgaagaga caattacttc tggaacccca ttgagatgct ggtaaaagcg ttggaaccac
     4321 aattccagat tcataccatt ggcgagaatc agttgtgccc cctgcgcacg tttaatatcg
     4381 ccaggggtag gctgatactc atgaatttct gcaccaggct tggttatgga tgagacttct
     4441 gcagcatctc cagccacgtt tttggccata tctgcgatga tggtaaaagt tgtaatgacc
     4501 ttgaattttt cagctgcact cgcctgaaat gctatcgagc aggtgaggac taggcacccc
     4561 aagagcatgg ttactttttt tattgagagc atgatttagt acctatagtg agcaaaatta
     4621 gaataaacat agcaactact atgttttata gcagaagcta taaaatgtaa ataattatca
     4681 ttagcgatta taagatccca acacagggat taggttattt cctgctgacg atgtggtatt
     4741 gaggataaaa gttgggcagc gctttggctt taaaatgaac ttgttcagga cgattggtaa
     4801 tggcaaagca aaaataatgt aggttattgg caagttctct aggacgcagg agagcatgaa
     4861 aaacccaggg gcagtcacta aatgaaggga
//
LOCUS       DBANUU010000231         4844 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000231 DBANUU010000000
VERSION     DBANUU010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.235"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            74..1990
                     /gene="mngA"
                     /locus_tag="ACIE68_004538"
     CDS             74..1990
                     /gene="mngA"
                     /locus_tag="ACIE68_004538"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFX5910723.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            2008..4641
                     /gene="mngB"
                     /locus_tag="ACIE68_004539"
     CDS             2008..4641
                     /gene="mngB"
                     /locus_tag="ACIE68_004539"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFX5910724.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEQIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLSERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         1182 a         1138 c         1343 g         1181 t
ORIGIN      
        1 aattgatcac ataatggtat tgttttatcg ggcacactgg cgcgactata aaaacgatca
       61 agtgaggatc atgatgaatc tgacgactct gacccaccgc gatgcgttgt gtctgaatgc
      121 gcgctttacc agccgtgaag aggccatcca cgcgttgact caacgtcttg ctgctctggg
      181 gaaaatttcc agtactgagc aatttctgga agaagtgtat cgccgtgaaa gccttggccc
      241 gaccgcctta ggtgaagggt tggctgtgcc gcatggcaaa actgctgcgg taaaagaagc
      301 ggcgtttgcg gtcgccacac tcagcgagcc gcttcagtgg gaaggcgttg atggcccgga
      361 agcggttgat ttagtggtgc tgctggcgat tccccccaat gaagcgggta ctacgcatat
      421 gcaactgctg acagcgctga ccacgcgcct ggcggatgat gagattcggg cgcgtataca
      481 gtcggcgacg acgcctgatg agttgctctc ggcgctggat gacaagggag gcacgcaacc
      541 ttctgcctct ttttccaacg cgccaactat cgtctgcgta acggcctgtc cggcgggtat
      601 tgctcacacc tatatggctg cggaatatct ggaaaaagcc ggacgcaaac tcggcgtaaa
      661 tgtttacgtt gaaaaacaag gcgctaacgg cattgaaggg cgtttaacgg cggatcaact
      721 caatagtgca accgcctgta tttttgcggc tgaagtcgcc atcaaggaga gtgagcgttt
      781 taatggcatt cccgcgcttt cagtgcctgt tgccgagccg attcgccatg cagaagcgtt
      841 gatccaacaa gcgcttaccc tcaagcgtag cgatgagacg cgtaccgtac agcaagatac
      901 gcaaccggtg aaaagtgtca aaacggagct gaaacaggca ctgttgagcg gaatctcttt
      961 tgccgtaccg ttgattgtcg cggggggcac ggtgctggcg gtcgcggtat tactgtcgca
     1021 aatcttcggg ctacaagatc tgtttaatga agaaaactcc tggctgtgga tgtaccgcaa
     1081 gctgggcggc gggctgctcg gaattttgat ggtaccggtg ctcgcggcct ataccgccta
     1141 ttctctggca gataaaccgg cgttagcgcc aggctttgcg gctggacttg ccgccaacat
     1201 gatcggctcc gggtttctcg gcgcggtcgt tggcggattg atagccggtt acttgatgcg
     1261 ctgggtgaaa aatcacttgc gtcttagcag taaattcaat ggattcctga ctttttatct
     1321 ctacccggtg ctcggtacgt tgggagcggg cagtctgatg ctgtttgtgg tgggagaacc
     1381 tgtcgcctgg atcaataact cgcttaccgc ctggctgaac ggtctgtcag gaagtaacgc
     1441 gctgttgctg ggtgccattc tcggttttat gtgttccttt gaccttggag ggccagtgaa
     1501 caaagccgct tatgcattct gcctgggcgc aatggcgaac ggcgtttacg gcccgtatgc
     1561 cattttcgcc tccgtcaaaa tggtttcggc atttaccgta accgcttcca cgatgctcgc
     1621 accgcgcctg tttaaagagt ttgaaattga gaccgggaaa tccacctggc tgttagggct
     1681 ggcaggtatt accgaagggg cgatcccgat ggcgattgaa gatccgctgc gggttattgg
     1741 ttcgtttgtg ctgggctcta tggtaacggg cgctattgtc ggtgcgatga atatcggcct
     1801 ttcgacaccc ggtgccggca ttttctcgct ctttttactt catgataatg gcgcgggcgg
     1861 tgttatggcg gcaattggct ggtttggtgc ggcattggtg ggggctgcaa tctcgactgc
     1921 aattctcctg atctggcggc gtcacgcggt taagcatggc aactatctga ctgatggcgt
     1981 aatgccataa acaaaaagga aacgacgatg aaagcggtat ctcgcgttca catcaccccg
     2041 catatgcatt gggatcggga gtggtatttc accaccgaag agtcacgcat tctgctggtc
     2101 aataatatgg aagagatcct gtgccgactg gaacaggaca acgaatacaa atattacgta
     2161 ctcgacgggc aaacggcgat cctcgaagat tatttcgcgg tgaaaccgga aaacaaagac
     2221 cgcgtgaaga aacaggtaga agccggtaag ttgattatcg gcccctggta tacccagacc
     2281 gataccacga ttgtttctgc ggaatccatc gtccgtaatc tgatgtacgg aatgcgtgac
     2341 tgcctcgcgt ttggcgagcc gatgaaaata ggttatttac cggattcctt tggcatgtcc
     2401 gggcaactgc cgcatatcta caatggattt ggcattaccc gcactatgtt ctggcgcgga
     2461 tgttcggagc gccacggtac tgataaaacc gagtttttat ggcaaagcag tgacggtagc
     2521 gaagtgacgg cgcaggtgct gccgttgggt tatgccatcg gtaagtactt acctgccgac
     2581 gaaaacggat tacgcaaacg cctcgacagt tattttgacg tgctggaaaa agcgtctgta
     2641 accaaagaga ttttattgcc gaatggtcat gaccagatgc cattgcagca aaatatcttc
     2701 gaagtgatgg agaaacttcg cgaaatttat cctcaacgaa agtttgtgat gagccgcttt
     2761 gaagaggtat tcgagcagat tgaagcgcag cgtgaaagtc tggcgaccct gaaaggtgaa
     2821 tttattgatg gcaaatatat gcgcgtgcat cgcaccatcg gttccacgcg catggatatc
     2881 aaagttgccc acgcacgtat tgaaaataag attgttaatc tgctggaacc gctggcaacg
     2941 ctggcctgga cgttgggttt tgaataccac cacggcttgc tggagaaaat gtggaaagag
     3001 atcttaaaaa atcatgccca cgacagtatc ggttgttgtt gcagtgacaa agttcatcgc
     3061 gaaattgtcg cccgcttcga actggctgaa gacatggcgg ataatctgct gcgtttctac
     3121 atgcgcaaaa ttgccgataa catgccacag agtgatgccg acaaactcgt gctgtttaac
     3181 ctgatgccct ggccgcggga agaagttatt aacaccactg tgcggctgcg cgccagccag
     3241 tttaatctac gagacgatcg cggtcagcct gtaccgtatt ttattcgcca tgcccgtgag
     3301 atcgatccag gcctaatcga tcggcaaata gttcattacg gtaattacga tccctttatg
     3361 gagtttgata tacagatcaa ccagattgtc ccttctatgg gctatcgcac gctttatatc
     3421 gaagcgaatc agcctggcaa cgtgattgcg gcaaaaagtg acgctgaagg gatactggaa
     3481 aatgctttct ggcaaattgc gctcaatgag gatggaacgc tacaactggt agataaagat
     3541 agcggtgtgc gctatgaccg ggtgttgcaa attgaagaaa gctctgatga tggtgatgaa
     3601 tatgactatt cacccgcaaa agaagagtgg gtaatgactt ctgcaacagc gaaaccgcaa
     3661 tgcgagatta ctcatgaggc ctggcagagc agggctgtta tccgctatga catggcggtg
     3721 ccgctcaatt tgtcagaacg cagcgtccgg caatccacgg gcagagtagg ggtggagatg
     3781 gttgtcactc tgagtcataa cagcagacgt attgatgtgg atatcaacct tgataaccag
     3841 gctgacgatc atcgccttcg cgtcctgatc cctacacctt ttaacaccga cagtgttctg
     3901 gcagatacgc agtttggttc gctaacgcgc cccgtgaacg acagtgcaat gaacaactgg
     3961 cagcaagaag gctggaaaga agcgccggtt ccggtatgga atatgctcaa ctatgttgcc
     4021 ttacaggaag ggcgtaacgg catggctgtc tttagcgaag ggctacgtga atttgaagtc
     4081 atcggtgaag agaagaaaac ctttgccatt acgttgctgc gtggcgtggg cttactgggc
     4141 aaagaagatc tgcttttaag gcctgggcgg ccttcgggaa ttaaaatgcc agtcccggac
     4201 tcacaactac gtggtctgct ttcttgtcgc ctaagtttat tgagttatac cggtacgcca
     4261 accgccgctg gtgtagctca gcaggcgcga gcatggctga ctccagtaca gtgttacaac
     4321 aaaatcccat gggatgcgat gaagctcaac aaagccggat tcaacgtgcc ggaaagttat
     4381 agtttgttga aaatgccccc agtgggatgc ctgataagcg cacttaagaa agctgaagac
     4441 cgacaagaag tgattttacg gctgtttaat ccggctgaat cagcaacctg tgatgcgact
     4501 gttgctttca gtcgcgaggt gatttcttgc tcagaaacga tgatggatga acacattacc
     4561 accgaggaaa atcaaggttc aaatctatcg gggccttttt tacccggcca gtcacggacg
     4621 ttcagttacc ggcttgcctg aatagcaatc aaaccgaagc cacatatgcg cggccagatt
     4681 gttgacaaag ggcgctttgt tcatgccgga tacggcatga acgctttatt cggtctacaa
     4741 aagcaggcaa attcaatata ttgcagagat gatgtagaca ctggcaagcg tagcgcatca
     4801 ggtaattttg cgtttatctt cactctcaag ccacgtatat gtgg
//
LOCUS       DBANUU010000232         4836 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000232 DBANUU010000000
VERSION     DBANUU010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.236"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            1244..1960
                     /gene="nanC"
                     /locus_tag="ACIE68_004540"
     CDS             1244..1960
                     /gene="nanC"
                     /locus_tag="ACIE68_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFX5910725.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1980..3086
                     /gene="nanM"
                     /locus_tag="ACIE68_004541"
     CDS             1980..3086
                     /gene="nanM"
                     /locus_tag="ACIE68_004541"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFX5910726.1"
                     /translation="MNKTITALTIIMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKRWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTTIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVASPDGVAGGFAGMSNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSADIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            3151..4131
                     /gene="nanS"
                     /locus_tag="ACIE68_004542"
     CDS             3151..4131
                     /gene="nanS"
                     /locus_tag="ACIE68_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFX5910727.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDGEDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMLGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGMDTPLYQDLVSRT
                     RVALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLDNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVKAILRFWRER"
     gene            complement(4139..4243)
                     /locus_tag="ACIE68_004543"
                     /pseudo
     CDS             complement(4139..4243)
                     /locus_tag="ACIE68_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            4357..4778
                     /locus_tag="ACIE68_004544"
                     /pseudo
     CDS             4357..4778
                     /locus_tag="ACIE68_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
BASE COUNT         1453 a          998 c         1069 g         1316 t
ORIGIN      
        1 tttatatatt tatcaatctg atacgaatct aataataacc ctcatcatta atgcaactaa
       61 tcttatctat atatcatgtg atatgttctg taacaagtaa aatcaacata aaaaattcag
      121 caaaatttaa tattcgttca actagccgtc tcacatatga cattattagt caactgcatt
      181 tgccacaaaa tggctatttc atggaacaca gtaatttctt agtggctatt atcaagctaa
      241 ttcttagatc cctagcgatt attcctgcct ggttcgtcag cttaggccat gagattgtta
      301 cattcctttt tatcctggag atatggggga acgcaggcga cgctttttca tctacatccg
      361 ttggtaacta tggctttggt cactacgact taaaccttcc tcaatcacag ctattcgccg
      421 ggcgatacgg caaacaaact aagtgaaaca tcatcctgaa aacaccaaca tcaacaagcc
      481 tctccagatc gacatcagga gtgaccagtt acaagccaca aaccaagaaa catatttcat
      541 gatgagaatt atgctcagcc tgatggcggg taaacactta ttttcaccaa gctcactgag
      601 ttagcttgca aagctttctt tatttagatt ctaaataaat tatttataaa acatatagtt
      661 aaaataaaat ctttagtttt tgaagaccac cgcaagtgtt cgtctggctt cacatggcat
      721 ctgcctctta gaaaaaggtc gacatatttt gtgacacgaa ttgcaaatct cgttttgttg
      781 tatggattgc gtgatttttg atctggtata acaggtataa aggtgcaccg cgatagtcaa
      841 tgagacaggg catctcgcaa tctatggcaa acatcacttc agttctttct catcgggtga
      901 tgaaaacgca cttcagtctg aaaggaatat gaaaatgaga tcaacagaca ctctatttta
      961 tgactctggg taaaatggat tgagtaagtg atatagctta cgaacattca aatcaattaa
     1021 acatcagaag agattttata ctcaggtatt taatctggtt ctctgtttat ttaaataatg
     1081 tggaaagaga attttcacag gagaccttat acaaaaaata taaaatacag ctactcgttg
     1141 ccaaagacac tataagcctg gcaaaaaata ttacacaaca taaatgctaa ttgtttatgc
     1201 gggctttgta ttgctttctg tatcctacaa atgagtgaaa tatatgaaaa aggctaaaat
     1261 actttctggc gtattattac tgtgcttttc gtcaccactg atttcccagg ctgcgacact
     1321 ggacgtacgt ggtggatatc gtagtggaag ccacgcctat gagactcgac tcaaagtcag
     1381 tgagggatgg caaaatggat ggtgggcaag tatggaaagt aatacctgga ataccattca
     1441 tgataataaa aaggaaaatg ccgcactcaa tgatgttcag gttgaagtta attacgcgat
     1501 taaacttgat gatcaatgga cggtgcgccc gggaatgtta acgcatttta gcagcaacgg
     1561 cacacgctac ggaccctacg taaaactgtc ctgggacgcg acaaaagatc ttaattttgg
     1621 cattcgctat cgttacgact ggaaagctta ccgacaacaa gacttatccg gtgatatgtc
     1681 tcgtgataac gttcatcgtt gggatggata tgtcacttac catattaata gtgatttcac
     1741 cttcgcatgg caaacgacgc tatacagcaa acagaacgat tatcgctatg caaaccataa
     1801 gaaatgggcg acggaaaatg catttgttct acaataccat atgacgcccg atattacgcc
     1861 atacatagaa tatgactacc ttgaccgtca gggtgtttac aacggcagag ataatttatc
     1921 ggaaaacagt tatcgcattg gtgtgtcatt taaactgtag tagacaggag acagtcacaa
     1981 tgaataaaac aataacggcg cttactatca taatggcttc atttgccgca aacgcgtctg
     2041 tattaccgga aactcctgtg ccatttaaaa gtggtaccgg agcaattgat aacgacactg
     2101 tctacattgg tttaggtagc gcaggtacgg catggtacaa gctggataca caggccaaag
     2161 ataaaagatg gacagcgtta gctgcattcc ctggcggacc gagagatcaa gcaacctctg
     2221 catttattga tggcaatctg tatgtgtttg gcggcattgg caaaaacagc gagggcttga
     2281 ctcaggtatt taatgacgta cacaaataca accccaaaac caatagctgg gttaaattga
     2341 tgtcgcacgc gccgatgggc atggcgggcc atgtgacttt tgtacacaac ggcaaggcct
     2401 atgttactgg cggtgttaac cagaatatct tcaatggcta ttttgaagat ctcaacgaag
     2461 ctggaaaaga ttcaaccact atagataaaa tcaacgccca ctattttgac aaaaaagcag
     2521 aagattattt cttcaataag tttctgttgt cttttgatcc ctctacacag caatggagtt
     2581 acgctggcga atctccctgg tacggaacgg ctggtgcggc ggttgtgaat aaaggtgata
     2641 aaacctggct tattaatggc gaagccaaac caggattgcg aacggatgcc gtatttgaac
     2701 ttgatttcac cggtaataat ttaaaatgga ataagcttgc tcccgtcgca tcaccagatg
     2761 gcgtcgctgg cggttttgca ggcatgagca atgactcact tatatttgcc ggaggggccg
     2821 gattcaaagg ttcacgagaa aattaccaga acggtaagaa ctatgcgcat gaaggcctga
     2881 aaaaatcata tagcgctgat attcatcttt ggcataacgg gaaatgggat aaatcgggcg
     2941 aattatcgca aggtcgggcc tacggagtat cattgccctg gaataatagt ttattaatta
     3001 ttggcggtga aactgcaggc ggcaaagcgg tgactgattc agttttgatc tctgtgaagg
     3061 ataataaagt cacagtacaa aactaacgct tcagggcccc ggtaaagggg ccctgctatc
     3121 aatttgtcat ttgtattacg cgaaaattat atgaacgcaa taatatcgcc cgattattac
     3181 tatgttctta ccgttgctgg tcagtctaat gccatggcgt atggcgaagg actgccatta
     3241 ccggacgggg aagatgcgcc tcatcccaga attaaacaat tagcgagatt tgcgcatacg
     3301 catcccggag gcccgtcatg tcactttaac gacattattc cactgactca ctgcccacac
     3361 gatgttcagg atatgctagg ttatcaccat cctctggcaa cgaatcatca aacacagtac
     3421 ggcaccgttg gccaggcact gcatatagca cggaaattac tgccctttat tcctgataat
     3481 gcaggggttc tcatcgttcc gtgttgccgt ggcggatcgg cttttaccgc gggcagcgaa
     3541 gggacatatt cagaacggca cggagccagc catgatgctt gtcgttgggg aatggatact
     3601 ccgctgtacc aggatttagt cagcagaacg cgagtcgcac tggcaaaaaa tccgcagaac
     3661 aaattcctcg gcgtatgctg gatgcaaggc gaatttgact taatgaccag tgactacgcg
     3721 tcacaccctc aacactttaa tcatatggtt gaagcctttc gtagggatct aaaacaatac
     3781 cattctcagc ttgataatat tactgacgca ccgtggtttt gcggcgatac cacctggtac
     3841 tggaaagaaa atttccctca tgcgtatgaa gcgatttatg gcaattatca aaataatgtt
     3901 ttagccaata ttattttcgt cgacttccag caacaaggtg aaagaggact gacgaacgcg
     3961 cctgatgaag atccggacga tttaagcacg ggatattacg gttcagcgta ccggtcaccg
     4021 gagaactgga cgacggcact gcgaagcagt catttcagcg cggcagcccg tcggggaatt
     4081 atttctgaca ggtttgtaaa agcaattttg cggttttggc gcgaaaggtg agcgttcatt
     4141 aatttgattt attcgcatcc atcagcgctt ttaactcctc cggcgtaaac ggcggtttgc
     4201 gtcggtgcaa catcgcatgg caattagggc acacaggaag taaaaaaaca tatgagatca
     4261 aggtaatata tcttcgcttg caggcttgaa cagcagaaaa tttgcgagca tgatcgcaat
     4321 tatttcactg ccagggatat caatggcttc cagtaaatcg ctgacgagcc tttttttgct
     4381 ctccagcaaa tccgtaagca gcgtgaccca ttatttcgca aacatgtatt acgcgcttat
     4441 aactaccagt gcgcaatatg tagctttaat atttgttacg gcaactccac cgtctcactg
     4501 gaagcgacac atataaaatg gaaacaacat ggtggccctt gtgaaattcc caatggcctg
     4561 gcgctttgcg cgatacatca taaagccttt gataaaggaa cgattggcct ggatgaaaat
     4621 atgcgggtac tggtattgga tgcggtaaac ggcggagaga tcattgagag gtcattctgg
     4681 gattttgacg gaaaaattat tgcgttgcca caggtgcgta aaaattaccc ttttgagggg
     4741 ttgtagagtg gcatcggaaa gagtttttta gggggtgagg tagccattat atatctttca
     4801 cctaggattt taaatgtaat agataaagta ttttct
//
LOCUS       DBANUU010000233         4790 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000233 DBANUU010000000
VERSION     DBANUU010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.237"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            244..585
                     /gene="raiA"
                     /locus_tag="ACIE68_004545"
     CDS             244..585
                     /gene="raiA"
                     /locus_tag="ACIE68_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFX5910728.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    688..813
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            689..736
                     /gene="pheL"
                     /locus_tag="ACIE68_004546"
     CDS             689..736
                     /gene="pheL"
                     /locus_tag="ACIE68_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFX5910729.1"
                     /translation="MKHTPFFFAFFFIFP"
     gene            835..1995
                     /gene="pheA"
                     /locus_tag="ACIE68_004547"
     CDS             835..1995
                     /gene="pheA"
                     /locus_tag="ACIE68_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFX5910730.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTCLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2038..3159)
                     /gene="tyrA"
                     /locus_tag="ACIE68_004548"
     CDS             complement(2038..3159)
                     /gene="tyrA"
                     /locus_tag="ACIE68_004548"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFX5910731.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3170..4240)
                     /gene="aroF"
                     /locus_tag="ACIE68_004549"
     CDS             complement(3170..4240)
                     /gene="aroF"
                     /locus_tag="ACIE68_004549"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFX5910732.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4450..>4790
                     /gene="yfiL"
                     /locus_tag="ACIE68_004550"
     CDS             4450..>4790
                     /gene="yfiL"
                     /locus_tag="ACIE68_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFX5910733.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADE"
BASE COUNT         1300 a         1237 c         1094 g         1159 t
ORIGIN      
        1 ccttgagctg ccgttttttt attctgtcag ttgtgaaact gaaccgattt agtcgctatc
       61 gatctcatca aatatggctc gctttgagat attcctcaag taaaaaaaca cctcttcctg
      121 cgatttctca caaaaaagat tcgttgacaa aaagtgacaa aattatgaga ttttcatcac
      181 acattttgac atcaggaacg gtatgctgaa ttcaccaaga cgggaagaca agaggtaaaa
      241 tttatgacaa tgaacattac cagcaaacaa atggaaatta ctccggccat ccgccaacat
      301 gtcgcagacc gtctcgccaa actggaaaaa tggcaaacac atctgattaa tccacatatc
      361 attctgtcca aagagccaca agggtttgtt gctgacgcca caatcaatac acctaacggc
      421 gttctggttg ccagtggtaa acatgaagat atgtacaccg caattaacga attgatcaac
      481 aagctggaac ggcagctcaa taaactgcag cacaaaggcg aagcacgtcg tgccgcaaca
      541 tcggtgaaag acgccaactt cgtcgaagaa gttgaagaag agtagtcctt tatattgagt
      601 gtatcgccaa cgcgccttcg ggcgcgtttt ttgttgacag cgtgaaaaca gtacgggtac
      661 tgtactaaag tcacttaagg aaacaaacat gaaacacaca ccgtttttct tcgcattctt
      721 ttttatcttc ccctgaaagg gaggcgtttc gtcgtgtgaa acagaatgcg aagacgaaca
      781 ataaggcctc ccaaatcggg gggccttttt tattgataac aaaaaggcaa cactatgaca
      841 tcggaaaacc cgttactggc gctgcgagag aaaatcagcg cgctggatga aaaattatta
      901 gcattactcg cagagcggcg cgaactggcc gtcgaggtgg gaaaagccaa actgctctcg
      961 catcgcccgg tacgtgatat tgatcgtgaa cgcgatttac tggaaagatt aattacgctc
     1021 ggtaaagcgc accatctgga cgcccattac attactcgcc tgttccagct catcattgaa
     1081 gattccgtat taactcagca ggctttgctc caacaacatc tcaataaaat taatccgcac
     1141 tcagcacgca tcgcttttct cggccccaaa ggctcctatt cacatcttgc cgctcgtcag
     1201 tacgctgccc gtcactttga gcaattcatt gaaagtggct gcgccaaatt tgccgatatt
     1261 tttaatcagg tggaaaccgg ccaggccgac tatgccgtcg taccgattga aaataccagc
     1321 tccggtgcca taaacgacgt ttacgatctg ctgcaacata cctgcttgtc gattgtgggc
     1381 gaaatgaccc ttaccatcga ccactgcctg ctggtttccg gcaccacaga tttatctacc
     1441 atcaacacgg tctacagtca tccacagccg ttccagcaat gcagcaaatt ccttaatcgt
     1501 tatccgcact ggaagattga atataccgaa agtacgtctg cggcaatgga aaaggttgca
     1561 caggcaaaat caccgcatgt tgctgcgttg ggaagcgaag ctggcggcac tttgtacggt
     1621 ttgcaggtac tggagcgtat tgaagcgaat cagcgacaaa acttcacccg atttgtggtg
     1681 ttggcgcgta aagccattaa cgtgtctgat caggttccgg cgaaaacgac gttgttaatg
     1741 gcgaccgggc aacaagccgg tgcgctggtt gaagcgttgc tggtgctgcg caaccacaat
     1801 ctgattatga cccgtctgga atcacgcccg attcacggta atccatggga agagatgttt
     1861 tatctggata ttcaggccaa tcttgaatca gcggaaatgc aaaaagcatt gaaagagtta
     1921 ggggaaatta cccgttcaat gaaggtattg ggctgttacc caagtgagaa cgtagtgcct
     1981 gttgatccaa cctgatgaaa aggtgccgga tgatgtgaat catccggcac tggattatta
     2041 ctggcggttg tcattcgcct gacgcaataa cacgcggctt tcactctgaa aacgctgtgc
     2101 gtaatcgccg aaccagtgct ccaccttgcg gaaactgtca ataaacgcct gcttatcgcc
     2161 ctgctccagc aactcaatcg cctcgccgaa acgcttatag taacgtttga ttaacgccag
     2221 attacgctct gacgacataa taatgtcggc ataaagctgc ggatcctgag caaacagtcg
     2281 cccgaccatc gccagctcaa ggcggtaaat cggcgaagag agcgccagaa gttgctcaag
     2341 ctgaacattt tcttctgcca gatgcagccc ataagcaaaa gtagcaaagt ggcgcagagc
     2401 ctgaataaac gccatattct gatcgtgctc gacagcgcta atacgatgca gccgagcgcc
     2461 ccagacctga atttgctcca gaaaccattg gtatgcttcc ggcttacgtc catcacacca
     2521 gaccacaact tgctttgcca ggctaccgct gtccgggccg aacatcgggt gtaaccccag
     2581 taccgggcca tcgtgcgccg ccagcatggc ctgtaatggt ccatttttca ctgatgccag
     2641 atcaaccaga atacaatctt tcggtaaagg cggtaatttg ccgataactt gctcagtaac
     2701 gtggattggc acactaacaa tcaccattcc ggcatcggca acaatatcag ccgctcgatc
     2761 ccagtcatgt tgctccagaa tccgcacctg ataacccgat agtgtcagca tcttctcgaa
     2821 caggcgtccc atctgaccgc caccgccgac gataaccacc ggacgcagtg acggacaaag
     2881 tgttttaaat cctttgtcgt tttcactgga gtaagattca cgcatcaccc gacgcaaaac
     2941 atcctcaatc agatctggcg gtacacccag agcttccgcc tctgcgcgcc gcgaggccaa
     3001 catagatgcc tcgcgctccg gaacataaat aggcagtcca aagcggcttt tcacctcgcc
     3061 cacttcagca accagttcca gacgcttcgc taataaattc agcagcgctt tatcgacttc
     3121 atcaatttga tcgcgtaatg cggtcaattc agcaaccata ataaacctct taagccacgc
     3181 gagccgtcag ctgcccgttc agatcctgat gaatttcacg cagcaaggca tcggtcattt
     3241 cccagctaat gcaggcatcg gttacggata caccgtattt catttcactg cgcggttgct
     3301 cggaagactg attgccctcg tggatattac tttcgatcat cagaccaata attgagcgat
     3361 tgccatcttt gatttgagca accacggatt ctgccaccgc aggctgacgg cgataatctt
     3421 tattggaatt accgtggctg caatctacca tcagagacgg gcgcagtccc gcctgttcca
     3481 tctctttttc acattgcgca acatccgcag ggctatagtt cggcgcttta ccaccgcgca
     3541 ggatcacatg gccgtccgga ttcccctgag tttgtagcaa cgcaacctgc cctgcctggt
     3601 taatgccaac aaaacggtgc ggctgggcgg cggcgcgcat agcgttaatt gctgttgcca
     3661 gactgccgtc ggtgccgttt ttaaaaccaa ccggcatgga aagcccggag gccatttcac
     3721 ggtgagtttg cgattccgtt gtacgagcac caattgctga ccagctaaac agatcgccca
     3781 ggtattgcgg gctattcgga tctaacgctt ccgtcgccag tggcagtccc atattcacca
     3841 gctcaagcag caatttacgc gcgatctgca gcccggcttc tacatcaaaa gagccatcca
     3901 tatggggatc gttaattaac cctttccagc cgacagtggt acggggtttt tcaaaataga
     3961 cgcgcattac cagatagagg ctatcgctga cctctgcggc aagggcttta aatcgacgag
     4021 catattccag agcagtttcc ggatcatgaa tggaacaagg accacatact accagcagac
     4081 gaggatcgcg cccggcgata atatctgaaa tggttttacg cgagtcagca atctgggctt
     4141 cttgttgcag gctcaatgga aaagcggcct tcagttgttc cggagtcatt aaaacctgtt
     4201 cgtcggtaat atgtacgtta ttcagcgcgt ctttttgcat gatggcgatc ctgtttatgc
     4261 tcgtttgcga tagttgatcc tcagcgagga tgacgtaacg ataacacata aagtaaagtt
     4321 ttcaatccat atttcgtaca tttttattta cacaggcaat ttagtcgcga tttcaaccct
     4381 tacttctgta tagataaatt tacactccct ttgaaaacaa tccgctatgc tttgaaaaag
     4441 gagaaagaaa tgatgaaaaa gtttatcgcc cccttgttgg ctttactggt tagcggatgt
     4501 cagattgatc cttatactca cgcgccaacc ttgaccagca ccgactggta tgatgttggt
     4561 atggaagatg cgatatcggg cagcgccata aaagatgacg atgcatttag cgattcacag
     4621 gcggatcgcg gtctatacct taaaggatat gccgaaggac aaaagaaaac ttgccagacc
     4681 gattttactt atgcccgagg actttccggt aaaagctttc ctgccagctg caacaatgtt
     4741 gaaaatgcca gccaactgca tgaagtatgg caaaaagggg ctgatgaaaa
//
LOCUS       DBANUU010000234         4788 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000234 DBANUU010000000
VERSION     DBANUU010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.238"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(21..397)
                     /gene="rnpB"
                     /locus_tag="ACIE68_004551"
     ncRNA           complement(21..397)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACIE68_004551"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(430..1575)
                     /gene="garK"
                     /locus_tag="ACIE68_004552"
     CDS             complement(430..1575)
                     /gene="garK"
                     /locus_tag="ACIE68_004552"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFX5910734.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1672..2562)
                     /gene="garR"
                     /locus_tag="ACIE68_004553"
     CDS             complement(1672..2562)
                     /gene="garR"
                     /locus_tag="ACIE68_004553"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFX5910735.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2592..3362)
                     /gene="garL"
                     /locus_tag="ACIE68_004554"
     CDS             complement(2592..3362)
                     /gene="garL"
                     /locus_tag="ACIE68_004554"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFX5910736.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     IDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     IEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3378..4712)
                     /gene="garP"
                     /locus_tag="ACIE68_004555"
     CDS             complement(3378..4712)
                     /gene="garP"
                     /locus_tag="ACIE68_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFX5910737.1"
                     /translation="MILDTVDVKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWSVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1127 a         1378 c         1161 g         1122 t
ORIGIN      
        1 agcgggtttt tacgtaaatc aggtgaaact gaccgataag ccgggttctg tcgtggacag
       61 tcattcatct aggccagcaa tcgctcactg gctcaagcag cctacccggg ttcagtacgg
      121 gccgtacctt atgaacccct atttggcctt gctccgggtg gagtttaccg tgccacggac
      181 tgttaccagc cgcgcggtgc gctcttaccg caccctttca cccttacctg atcccgcttg
      241 cgcgggccat cggcggtttg ctctctgttg cactggtcgt gggtttcccc cccaggcgtt
      301 acctggcacc ctgccctatg gagcccggac tttcctcccc tccgcccgtc tcccccgaag
      361 aggacgacga cgaagcggcg actgtctggt cagcttcggc gcgcagtata gagggtttgc
      421 gcgcccttgt caccccgcgt tgcgcattcc aatcgccagt gtcgcggcga tattacgtga
      481 agcacggcag atattgtcat aagccccgcg gaatgcttcg tccaacgtac ctatgctggt
      541 caatacgctg aagaccgcat caatgccatg ctgatgtaca acgccaacat catcggtcag
      601 gctacccgca atgccaatca ccggtttatg gtacttcttc gccacgtttg cgacaccaat
      661 cggtaccttc ccgtgaatac tctggctgtc aatacgccct tcaccggtga tcaccagcgt
      721 acaatcgtga atatgttcct ccagattcag cgccgtagtg acgatttcaa taccactttt
      781 cagttccgca ccaagaaacg ccattagcgc cgcgcccata ccacccgcag ctcctgcacc
      841 ggggacatct ttcacatcaa catgcagcgc ttttttaatg acctcggcat agtgagagag
      901 gttattgtcc agctcaacaa tcatcgcttc actggctccc ttttgtgggc caaagatgcg
      961 cgatgcgccg ttatcgccca ccagcggatt ggtgacatca caagcgacgc gaatgacgca
     1021 atcttttaag cgcggatcga ggccggaaat atcaatatca ttcagagtat taagactacc
     1081 gccgccaaaa ccaatttcat tgccgttggc gtcgcataat ttcgccccca gcgcctgtac
     1141 catgcctgcg ccgccatcat ttgtagcgct accgccaatg ccgataataa tgtttgtcgc
     1201 gccgctctcc agcgcctgca ggattaactc gcctgtgccg cgtgaagtgg tcacgagtgg
     1261 atcgcgtttt tccgcaggta ccagctccag cccactggcc gccgccattt caataaacgc
     1321 ggttttgcca tcgccggaga tcccccaact ggcattcact ttctcgccca gcggccctgt
     1381 aacccaggcg tgacgttcag aaccctgggt ggctgcaatc atcgcttcca ccgttccttc
     1441 gccaccgtcg gcaaccggaa tagaaacgta ctgtgcatca ggaaaaattt cccgaaatcc
     1501 tttttctatc gcctgcgcaa cctcgctggc agataaactt tctttataag agtctggggc
     1561 gattacgatt ttcataccta tgcctgttac cacatgacgc cggagggcgt ttctcttatt
     1621 cggcctggat tccaggcccg gattgcaata cgccatccgg gcacgacgtc attaacgagt
     1681 aacttcgact ttcgccagtt tttcgtagta gcacgccagg gcgctatgat ccgccgttcc
     1741 taaaccatct gctcgcagtg cctgcatcat ctccataacc gcagctgtga gcggcagttg
     1801 cgcgccgacg ccgtgggaag tatccagcgc atttgccaga tccttaatat gcagatcaat
     1861 acggaagccc ggcttgaagt tgcggtccat caccatcggc gctttggcat ccagcacggt
     1921 actgcccgcc agtccaccgc gaattgcctg ataaaccagg tccgggttaa cgcccgcttt
     1981 ggttgccagc gttagcgctt ctgacatcgc ggcaatattc agcgccacaa tgacctgatt
     2041 tgccagtttg gtgacgttac ctgcaccgat ttccccggta tgcaccacgg aacccgccat
     2101 cgctttcatc aaatcatagt atttgtcgaa aatagccttg tcgccgccca ccatcactga
     2161 cagcgtaccg tcgatggctt tcggttcacc gccgctcacc ggagcatcca gcatatcaat
     2221 gcctttcgct ttcagcgctt cgctgatttc acggcttgcc agcggtgcga tagaactcat
     2281 atcgatcaat accgtacctg gcttcgcgcc ttcaatgatg ccattctcac ccagcgccac
     2341 ctctttcaca tgaggggagt ttggcagcat ggttatgatg acgtcgcact gttcagcgat
     2401 cgctttagcc gtagacgctg tttctgcacc tgcagcaatc acgtcagcaa tagcttctgg
     2461 gttacggtca gcaaccacca gcgagtaacc tgctttcaga aggtttttac tcattggttt
     2521 acccataatc cccaggccaa taaaaccaac tttcatagtc atatcaatca tctctcttgt
     2581 tgcggtggtg gttatttttt aaaggtatca gccagtttct gagtggcaga gcggaagacg
     2641 ccgagatcgc tgccgacagc cacaaacgtc gcgccccatt ccagataacg acgcgcatcg
     2701 gcttcgatcg gtgcgaggat accgctgggt ttgccgtgcg cactggcacg gttaaaaatg
     2761 tgctgaattg ctttttgtac atccgggtgc gaggcattgc cgagatggcc taatgccgca
     2821 gccagatcgc tggggccgac gaagatgccg tctacgcctt cggtagcggc aatggcatcg
     2881 atgttatcta cgccctgctg actttctatc tggaccagaa tagtgatgtt cttgttcgac
     2941 tgagcgaaat aatccgccac ggtgccaaac atattggcgc ggtgagaaac ggagacgccg
     3001 cgaatgcctt ccggtgggta acgggttgat gccaccgcct gctctgcttc ctcttttgtt
     3061 tctacaaaag gaatcaggaa gttatagaaa ccgatatcca gaagacgctt aataattacc
     3121 ggctcgttgg tcggcactcg cactactggc gcgctggcgc tgcctttcag ggccattaac
     3181 tgcggaataa acgtggaaat atcgtttggc gcatgttcgc catccagcac cagccagtca
     3241 aacccagcca aaccaagaac ttcagtgcta atcgggttag agagtgctga ccagcaacca
     3301 atttgtacct gtttcgcagc cagtgcggct ttgaatttat tcgggaaaac atcgttattc
     3361 atcgcttata cctttgctta tttctgcaat tccatacgtt taatgtcgcc aactacgaag
     3421 aggtagcaga ccatcgccat cagcgctgaa catcccacga aaaccagtgc tgcattgaag
     3481 gagtgcagtt cacttaccag gtagccaatc accagtggag tgacaatgga ggcaacattg
     3541 ccaaagacgt taaagacgcc gccgcagagg ccaacaatct ctttcggcgc ggtgtcagaa
     3601 atcaccgacc agcccagagc accaaatcct ttgccaaaaa aagccagcgc catcagcatg
     3661 accaccagcg tggtgttgtt ggtgtagtta cataagatga tggtggaagc cagcaacatt
     3721 cccagcacaa tcggtagctt acgtgccagg gtcagggata aaccgcgttt gatcagataa
     3781 tccgagaaga cacctcccag cacgccgccc gcaaaaccac acagtgccgg aatcgaggcg
     3841 accagaccca ctttcagaat cgacatgcct ttttcctgca ccagataaat cgggaaccag
     3901 gtgaggaaga accaggtgat ggtgttgata aaatattgtc cgaaaaatac gcccaacatc
     3961 atgcggttag agagcaattg cttgatgtaa tgcagtttgg gaccgcttgc tgccgcgctg
     4021 cccggctttt tgtggtccat atcgaccacc gcgccatttt cagagataaa cttcagctct
     4081 tccgcagaca tacgtgggtg atctgtcggg ttatgaatca acttgatcca cagcgccgtc
     4141 agcacaaaac caatcacccc cataacggtg aagacgtgct cccagcccca ggcgaaagtc
     4201 agccagccaa gcagcggcga aaagagcgcc agcgagaaat attgcgccga gttaaagatg
     4261 gcggaggcag taccacgttc tttcgtcggg aaccaggcgg cgacaatacg ggcgttcgcc
     4321 gggaatgatg gcgcttccga gaaccccagc ataaagcgca taaagaacat ggagatccct
     4381 gcccaggcca gcgggaacat atcaacaaag ccttgcagga aggtgaacag cgaccagaaa
     4441 aagaggctgt aggtgtaaac ttttttcgag ccaaacttat caagcagcca gccgccggga
     4501 atttgcatca gcaggtaggc ccagccaaaa gcggagaaga tgtaacccat cgaaaccgca
     4561 cttaactgca actcttttgc cacttcggta ccagcaatag acagcgttgc acgatcggcg
     4621 tagttaacgg cggtaacaat aaaaataatc agtaatatta aatagcgggt atgcacgcct
     4681 ttctttttta cgtcaacggt gtccagaatc attttattta cctcgggtac ttatgctgat
     4741 ttttattatt atggtgaagg tgttatttat gagttttatt tatgccgt
//
LOCUS       DBANUU010000235         4766 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000235 DBANUU010000000
VERSION     DBANUU010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.239"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            99..1073
                     /gene="hemB"
                     /locus_tag="ACIE68_004556"
     CDS             99..1073
                     /gene="hemB"
                     /locus_tag="ACIE68_004556"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFX5910738.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1179..2030)
                     /gene="tauD"
                     /locus_tag="ACIE68_004557"
     CDS             complement(1179..2030)
                     /gene="tauD"
                     /locus_tag="ACIE68_004557"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFX5910739.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2027..2854)
                     /gene="tauC"
                     /locus_tag="ACIE68_004558"
     CDS             complement(2027..2854)
                     /gene="tauC"
                     /locus_tag="ACIE68_004558"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFX5910740.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTLSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2851..3618)
                     /gene="tauB"
                     /locus_tag="ACIE68_004559"
     CDS             complement(2851..3618)
                     /gene="tauB"
                     /locus_tag="ACIE68_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFX5910741.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSILLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLSLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3631..4593)
                     /gene="tauA"
                     /locus_tag="ACIE68_004560"
     CDS             complement(3631..4593)
                     /gene="tauA"
                     /locus_tag="ACIE68_004560"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFX5910742.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1094 a         1384 c         1230 g         1058 t
ORIGIN      
        1 cattctcttc ttgtcaacat cacgacaact ttcgtaaaac actcctgccc cgcttcaggt
       61 atactgtgca ccttaattcc acaaacatca ggcagaccat gacagactta atccaacgcc
      121 ctcgtcgcct gcgcaaatct cctgcgctgc gcgctatgtt tgaagagaca acacttagcc
      181 ttaacgacct ggtgttgccg atctttgttg aagaagaaat tgacgactac aaagccgttg
      241 aagccatgcc aggcgtgatg cgcattccag agaaacatct ggcacgcgaa attgaacgca
      301 tcgccaacgc cggtatccgt tccgtaatga cttttggcat ctctcaccat accgatgaaa
      361 ccggcagcga cgcctggcgg gaagatggac tggtggcgcg tatgtcgcgc atctgcaagc
      421 agactgtgcc agaaatgatc gtcatgtcag acacttgttt ctgtgaatac acttctcacg
      481 gtcactgcgg tgtgctgtgc gagcatggcg tcgacaacga cgcgactctg gaaaatttag
      541 gcaagcaagc cgtggttgca gctgctgcag gcgcagactt catcgcccct tccgccgcga
      601 tggacggcca ggtacaggcg attcgtcagg cgctggacgc tgcaggcttt aaagatacgg
      661 cgattatgtc gtattcgacc aagttcgcct cttcctttta tggcccgttc cgtgaagctg
      721 ccggaagcgc attaaaaggc gaccgcaaaa gctatcagat gaacccaatg aaccgtcgcg
      781 aggcgattcg tgagtcactg ctggatgaag cccagggcgc agactgcctg atggttaaac
      841 ctgctggagc gtacctcgac atcgtgcgtg agctgcgtga acgtactgaa ttgccgattg
      901 gcgcgtatca ggtgagcggt gagtacgcga tgattaagtt cgccgcgctg gcgggtgcta
      961 tagatgaaga gaaagtcgtg ctcgaaagct taggttcaat taagcgtgcg ggtgcggatc
     1021 tgattttcag ctactttgcg ctggatttgg ctgagaagaa gattctgcgt taattttttt
     1081 cctctcagaa agggggagtt aacctgttga aagtacggtg aacgccccct caccctaacc
     1141 ctctccccaa gggggcgagg gaaccgtccg cactcgtatc accccgcccg ataaaacggt
     1201 ttatccccaa gaatcgtcgc ccgatgcata atccgccgct gtggcaggta atcggcattg
     1261 gcatagtgct gagtcacgcg gttatcccaa atcgcaatat cattcggttg ccagcgccaa
     1321 cgcacctgaa actccggttt agtgatatgg gcgaataaaa agcctaacaa ggcttcgctc
     1381 tctttctcgc tgacatcgac aattcgcgta gtaaagcctt cattcacaaa cagcgcctgt
     1441 ttaccgctca ccggatgcgt tcgcaccacc ggatgtagca acggaggatt tttcgcgacc
     1501 gcctcgcgcc agcgttgatg ttcctcctcg gttttgcggt atttgtattc cgggaacgat
     1561 ttacggaaat catgctccgc acgcagcccg ctcagcaact ggcggaaggg aacagagagc
     1621 gcctcatagg ccgcaatacc gctggtccag agcgtatcac cgccggtcga aggtaattct
     1681 ttagctgcca gaatcgcccc tgcgggtggc gtttcaataa atgtcacatc ggtatgccag
     1741 ttgtcgttat ctggcggatt atcgttatgg gtatccagca cgatgatctc gtcaacccct
     1801 tcggcatgag ggtaaacagg gtgaatatgc agttcgccaa aacgctgggc cagcgcgcgt
     1861 tgctgctgcg gcgtaatggc ttgatcgcgc agaaacacca cctgatggcg cagcaccgca
     1921 tggtaaagct gttcaaactg attatcgctt aacgggcgcg tcaggtcggc acccgaaatt
     1981 tgtgcgccga tatacggccc cagcggggta atgctcagac gttcactcat tgtacttctc
     2041 catgccaggg cgtcagacgg cgctgtaacg cgcgcaaacc cagttctaaa agaaaggcga
     2101 taatcgcaat caccgcgatt cccgccagca ccacgtcagt cgcgagaaat tcacccgctg
     2161 actgaaccat aaatcctaaa ccgcgcgtcg cggcaatcag ctccgccgct accagcgtag
     2221 accagcccac acccagacca atgcgtaatc cggtgaggat ttccggcagc gcgccgggca
     2281 aaatgacaaa ccacagcacc tgcgcacggc tggcacccag cgactgggcg gcacgaatgc
     2341 gaacctgctg cgcgcttttc acccccgcca gcgccgacat cgccaccggt gcaaaaatcg
     2401 ctaaatagat cagtaagatc ttcgaggttt caccaatacc aaaccagatc accatcagcg
     2461 gcaaataagc cagcggcggc accggacgat aaagttcgat taccggatcc agaatgccgc
     2521 gcaccgtcgg gcttagcccc atggcgatcc cgaccggaat accgatcaac accgccgcga
     2581 gtagcgccag cacaatgcgc gtcaggctgg ctgccagatg ctgccacagc gtggcgtcca
     2641 taaatccctg cggcccggca atggtgagca gttttgccag tacctgttgc ggtggcggca
     2701 aaaacagcgg gctaatcagt tgcagcgctg cgactgccca ccatacagta agtaaaaccg
     2761 ctaacgtgcc aatgcttaag gtcacctgac gcgagagcgg ccagcgccat ttaagccgat
     2821 gcgaatgcag tttttcatta atgagcacac tcatgagaac gcctcccgct gctcaaatac
     2881 gcggcttaaa acatattcgc gcatggcgat gaattgtgga tcggacttga tgctgcggct
     2941 cgactctccc gcaacaaagc ggcgagcaaa gttgagcgac agccgctcca gcacacggcc
     3001 agggccggat gaaagcagaa ccagttcagt cgccataaac accgcttctt ctatatcgtg
     3061 ggtaatcaac agcacctgct tgcccgtctc ctgccagagt ttcagcagca gggtttgcat
     3121 ctggtcgcgg gtgaaggcgt ccagcgcacc aaacggttcg tcgagtaata acagctgggg
     3181 attcgccgcc agcgcacggg caatccccac ccgctgacgt tgaccaccgg aaagctgcca
     3241 gatgtagcgt ttttctgcgc cttccagccc cacttttttc agcatctggt gcgcgatttc
     3301 cagtcgctgc attttctcta tacctgccaa ttgcaggccg aacgccacgt tgtcctgtac
     3361 attgcgccac ggtagtagcc cttcattctg aaaaactacg ccacgctctg ctcccggtcc
     3421 ctcaatacgc ttacccgcca gtagaatgct gccatgctga taaggcacaa aaccggcaat
     3481 cagattcagc aacgtggttt taccgcagcc ggacggcccc agcaccacca gtagctcgcc
     3541 gctttccagc gtcaggttga tatcttccag tgccggtttg ccgccataat cggcgtaaag
     3601 atgagagatt tgcagcatct gcgcctcctt ttattgcacg aagcgcgagg taacgtactg
     3661 gctgtaatca ttcgctacag ccgggacctt gccctgctct ttcaaaaact gcgcggtgtc
     3721 gatgatcgct ttgttcaccg gtccggtcag ttctgccgtt tgttgctgcg gcgtcagata
     3781 ggtattcccc ttcaccagcc ccggaacgtc accttcaggc acgccgctta aacgcgccag
     3841 tttgctgatg ttttccggct gtttcagcca cacgtctggg ttagcaatgt acggttgctg
     3901 agcatcgatg gcgcttttag cgaacgcttt cacgacctca ggatgtttct cggcaaaatc
     3961 tttgcgcacc acccagacgt ccagcgttgg cgcgccccac tgcccgacct gttcagaatc
     4021 ggtcaacacc ttgccgtctt tttccagggc gttaaccgcc ggtgcccaga cataagcacc
     4081 atcaatatct ccccgctgcc aggcagcgat aatcgcgggc ggctgcaggt tcacaatctc
     4141 cacttgcccg ggtttaatgc cccagtgttt cagcgccgcc agcaggctgt agtgggtggt
     4201 ggagataaac ggtacggcga tgcgtttgcc aatcagatct tccggtttgc tgatagtttt
     4261 ctttaccacc agcgcttcgg agttacccag ttttgacgcc agcaagaaga cttcaatcgg
     4321 cacctgttgg ctggctgcaa ccgctaacgg gctggaaccg aggttgccga tttgcacatc
     4381 gcctgaagcc agcgcccgca cgatgctggc tccgctgtca aacttacgcc agtccacgct
     4441 cgccccgctc tctttagcaa aggtgttgtc ggcctgagcc actttcgccg gttcggctga
     4501 ggtttgatag gccacggtga cgttcaccgc ctgtgcctga aaagcgatga atgccagtgc
     4561 ggctagaagt gtgttacgcg atgaaattgc catgattgtc tgctcccctg tcttgttatg
     4621 ggagcagtat tcaggaataa aaacattcat taaaagaatt agtcgttatc gcacagatga
     4681 ttttattctt agcaaaaaaa cggctaagga aagattaaat atgcaattac tccgttctaa
     4741 caattgggaa aattactaca cttcat
//
LOCUS       DBANUU010000236         4693 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000236 DBANUU010000000
VERSION     DBANUU010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.240"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..4551
                     /gene="traI"
                     /locus_tag="ACIE68_004561"
     CDS             <1..4551
                     /gene="traI"
                     /locus_tag="ACIE68_004561"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFX5910743.1"
                     /translation="IREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAEWMQTLKET
                     GFDIRAYRDAADQRTEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLAR
                     TVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIM
                     KQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMARE
                     QGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLK
                     ETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEP
                     DRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMT
                     ALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGET
                     QVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVV
                     VPGRAEPASLPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLA
                     RSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISLQKAGLHTPA
                     QQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKRGDLLYVDV
                     AKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI
                     LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAVGEMRSAGV
                     DAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVA
                     SGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINRDVERALSG
                     LESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDIPMTLYEAI
                     VRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVPVLNTANIR
                     DGELRRLSTWEKNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEG
                     VTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPG
                     QERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQ
                     VYTDNRQGWTDAINNAVHKGTAHDVFEPKPDREVMNAQRLFSTARELRDVAAGRAVLR
                     QAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAGIWLNPLTTDDGNGLRGFSGE
                     GRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRITGEGRPWNPGA
                     ITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENK
                     PDLPDGKTELAVREIAGQERDRTATSEREAALPESVLRESQREREAVREVARENLLQE
                     RLQQMEQDMVRDLQKEKTLGGD"
     gene            4571..>4693
                     /locus_tag="ACIE68_004562"
     CDS             4571..>4693
                     /locus_tag="ACIE68_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFX5910744.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVAL"
BASE COUNT         1048 a         1241 c         1596 g          808 t
ORIGIN      
        1 atccgggagg ccgtcgggga agacgcctcg ctgaaatccc gggatgtggc ggcactggat
       61 acgcgtaaat ccaaacagca cgtcgatccg gaaatcagaa tggctgaatg gatgcagacg
      121 ctgaaggaaa ccgggttcga catccgggca tatcgtgacg cggcggatca gcgtacggag
      181 atccgcacgc aggcgcccgg gccagcttca caggacgggc cggatgtgca gcaggcggtg
      241 acacaggcga ttgccggatt aagtgaacga aaagtgcagt tcacgtacac ggacgtgctg
      301 gccaggacgg tcggcatact gccgccggaa aatggtgtga ttgaacgggc acgcgccggt
      361 atcgatgagg ccatcagccg tgagcagctt atccccctcg atcgtgagaa ggggctgttc
      421 acgtccggga ttcatgtgct cgatgagctg tcagtccggg cactcagtcg tgacatcatg
      481 aaacagaacc gggtgaccgt acatccggag aaaagtgtcc cccggacggc cggttacagc
      541 gatgccgtca gtgttctggc acaggatcgc ccgtcgctgg ccattgtgtc cgggcagggc
      601 ggtgcagccg ggcagcgtga gcgggtggct gaactggtca tgatggcccg ggaacagggg
      661 cgggaggtgc agattatcgc tgctgaccgt cgctcgcaga tgaacctgaa gcaggatgaa
      721 cggctgtccg gtgaactgat aaccggacgt cgtcagctgc tggaaggcat ggccttcacg
      781 ccgggcagta ccgttatcgt cgaccagggc gaaaaactct ccctgaaaga gacgttaacc
      841 ctgctggatg gtgccgcacg tcataacgta caggtcctga taaccgacag cgggcagcga
      901 accggtacag gcagtgcgct gatggccatg aaggatgccg gggtgaacac atatcgctgg
      961 cagggcggag aacagcgacc ggccaccatc atcagtgaac cggaccgtaa tgtccgctat
     1021 gcccggctgg ccggagattt tgcggccagc gtgaaagccg gagaagagag cgtggcacag
     1081 gtcagcgggg tacgggaaca ggccatactg acacaggcca ttcgcagtga gctgaaaacg
     1141 cagggcgtgc tcggacaccc tgaggtgacc atgactgcac tgtcaccggt ctggctggac
     1201 agccggagcc gttatctgcg ggatatgtac cgaccgggga tggtgatgga gcagtggaac
     1261 ccggagacac gcagtcatga ccgctatgtg atagaccggg tgacagcgca gagtcacagc
     1321 ctgaccctgc gggatgcgca gggcgaaacg caggtggtgc gtatttcctc cctggacagc
     1381 agctggtcgc tgttccggcc ggaaaaaatg ccggtggcag acggcgagcg actgagggtg
     1441 acagggaaaa ttcccggact ccgcgtctcc ggcggtgacc gcctgcaggt ggcatctgtc
     1501 agtgaagatg cgatgacggt tgttgtgccg gggcgggctg aaccggcctc cctgcctgtg
     1561 agcgattcac cgttcacggc actgaagctg gagaacggct gggtggaaac gcccgggcat
     1621 tccgtcagtg acagtgcgac ggtgtttgcc tccgtcacac agatggcaat ggacaacgcc
     1681 accctgaacg gtctggcccg cagcggtcgt gatgtccggc tgtattcctc actggatgaa
     1741 acccgtactg cggaaaaact tgcccgccat ccctccttta cggtggtttc tgagcagata
     1801 aaggcgcggg ccggtgagac atcgctggaa accgctatca gtctgcagaa agccgggctg
     1861 cacacgccgg cacagcaggc cattcatctg gcccttcctg tgctggaaag taaaaacctg
     1921 gccttcagca tggtggacct gctgacagag gcgaagtcgt ttgctgcaga aggaaccggt
     1981 tttactgaac tgggagggga aatcaatgcg cagataaaac gcggtgactt actgtatgtg
     2041 gatgtggcaa aaggctatgg cacaggcctg ctggtttccc gtgcgtcgta tgaggcagaa
     2101 aaaagtattc ttcgccatat tctcgaaggt aaggaggcgg tcacgccgct gatggagaga
     2161 gtacccggcg aactcatgga gacgttaacg tcgggacagc gtgccgccac ccgtatgata
     2221 ctggaaacgt ccgaccgctt cacggtggta cagggttatg ccggtgtggg taagaccaca
     2281 cagttccggg cggtgatgtc agccgtgaac atgctgccgg cgagtgagcg tccccgagtt
     2341 gtggggctgg ggcccacgca ccgcgcggtc ggggagatgc gcagcgccgg cgtggatgca
     2401 cagacgctgg cgtcctttct gcatgacacg cagctgcagc agcgcagcgg agaaacgccg
     2461 gatttcagca acacgctgtt cctgctcgat gagagctcaa tggtgggcaa taccgacatg
     2521 gcacgggcat acgccctgat tgcggccggt ggtggtcgtg ctgtggccag cggtgacacg
     2581 gaccagctgc aggccatcgc gcccggtcag cctttccgtc tccagcagac gcgcagtgct
     2641 gccgatgtgg ccatcatgaa ggagattgtg cgtcagacgc cggaactgcg ggaggcggta
     2701 tacagcctga ttaaccggga tgtggaaagg gcactgtccg ggcttgagag tgtgaaaccg
     2761 tctcaggtgc cacgtctgga gggcgcatgg gcaccggagc actccgtgac ggagttcagt
     2821 cacagccagg aagcgaaact ggcagaagcg cagcagaagg cgatgctgaa aggcgaggct
     2881 ttcccggata tccccatgac actgtatgag gccattgtcc gcgactatac cggcaggaca
     2941 ccggaagcac gggagcagac gctgattgtc acgcacctga atgaggaccg gcgcgtactg
     3001 aacagcatga ttcatgatgc acgggaaaag gccggtgagc tgggcaaaga gcaggtcatg
     3061 gtgcctgtcc tgaacacagc gaatatacgt gacggagaac tgcgtcgtct ctccacctgg
     3121 gagaaaaacc cggatgccct tgccctggtg gataatgtgt atcaccggat tgccggtatc
     3181 agcaaggatg acgggctgat aaccctgcag gatgcggaag gtaacacgcg gctgatttcg
     3241 ccccgggagg cggtggctga aggtgtcaca ctgtacacac cagacaaaat ccgggtgggg
     3301 accggtgacc ggatgcgctt cacgaagagt gaccgggagc gcggttatgt ggccaacagc
     3361 gtctggacgg tgacagcggt ttccggtgac agtgtcacgc tgtcggacgg acagcagacc
     3421 cgggtgattc gccccggaca ggagcgggca gagcaacata ttgacctggc ctatgccatc
     3481 accgcccacg gtgcgcaggg tgcgagtgaa acctttgcca tcgcgcttga aggcacggaa
     3541 ggtaaccgga aactgatggc cggctttgag tcagcctacg tggctctgtc gcgtatgaag
     3601 cagcatgtgc aggtgtacac cgataaccgt cagggctgga cggatgccat taacaatgcc
     3661 gtacataaag gaactgccca cgatgtgttt gagccgaaac cggaccggga ggtcatgaat
     3721 gcgcagcggc tgttcagtac ggcgcgggag ctgcgggacg tggcggcagg ccgtgccgtt
     3781 ctccgtcagg cggggcttgc cgggggagac agccctgcac ggtttattgc accgggacgt
     3841 aaatatccgc agccgtatgt ggcactgccg gcgtttgacc gtaacggcag gtccgccggt
     3901 atctggctga acccactgac cacggatgac ggaaacggac tgcggggatt cagcggtgaa
     3961 ggccgggtga aaggcagcgg ggatgcacag tttgtggccc tgcagggcag ccgtaacgga
     4021 gagagcctgc tggctgataa tatgcaggat ggtgtccgga ttgcccgtga taatcctgac
     4081 agcggtgtgg tggtgagaat taccggtgaa ggtcgtccgt ggaatcccgg tgccatcacc
     4141 ggtggccgcg tgtgggggga tatcccggat aacagcgtcc agccgggggc cggaaatggc
     4201 gaaccggtca cggcagaggt actggcacag cggcaggctg aagaggccat ccgccgtgaa
     4261 acggaacgcc gcgcagatga aattgtcaga aaaatggcag agaacaaacc tgacctgccg
     4321 gatggcaaaa cagagctggc tgtcagggag attgccgggc aggaacgtga ccggactgcc
     4381 acttctgaac gggaagccgc gctgccggag agtgtgctgc gtgaatcaca acgggagcgg
     4441 gaagcggtcc gggaggttgc ccgggaaaat ctgctgcagg aacgacttca gcagatggag
     4501 caggatatgg ttcgtgacct gcagaaagag aaaaccctgg gcggagactg atacaggaag
     4561 acaaacactg atgacaacgg ataacacgaa cacgacacgt aacgattcac tggctgcccg
     4621 gaccgatacc tggttgcagt cgttgctggt ctggtcaccc ggacagcggg atatcatcaa
     4681 aacggtggca ctg
//
LOCUS       DBANUU010000237         4688 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000237 DBANUU010000000
VERSION     DBANUU010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.241"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..2489
                     /locus_tag="ACIE68_004563"
     CDS             <1..2489
                     /locus_tag="ACIE68_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFX5910745.1"
                     /translation="LTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAEDRENVN
                     LSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTLLGFTDE
                     IIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPPELWDHG
                     IAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFNNGKSQA
                     NNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPPDLRGYA
                     PQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEEDGQVTQ
                     WQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNVSLYGSS
                     VLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSKRFESTG
                     SQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLNTYVSLA
                     RNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNISIPWGT
                     SRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRASYQHNT
                     ENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDADGAEDIP
                     LNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEGAIGYAK
                     LDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQDDSKLTL
                     RWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            2505..3254
                     /locus_tag="ACIE68_004564"
     CDS             2505..3254
                     /locus_tag="ACIE68_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFX5910746.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3256..4320
                     /locus_tag="ACIE68_004565"
     CDS             3256..4320
                     /locus_tag="ACIE68_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5910747.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(4363..4563)
                     /gene="glgS"
                     /locus_tag="ACIE68_004566"
     CDS             complement(4363..4563)
                     /gene="glgS"
                     /locus_tag="ACIE68_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFX5910748.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT         1447 a          976 c          939 g         1326 t
ORIGIN      
        1 aattaaccac aataagcgaa ttgattaaaa atatttattg ttcattatcc gttctcatca
       61 ttggttgtgc gtcagcttat gccgttgaat tcaacaaaga tttaatcgaa gccgaagatc
      121 gtgaaaacgt taacctttcc caatttgaaa ctgatggcca attacccgta ggtaaatact
      181 cattaaacgc tcttattaat aataaaagga cgccaatcca tctggacctg caatgggtat
      241 taattgataa ccaaactgca gtttgtctga ctccagagca attaacatta ttaggcttta
      301 ctgatgagat tattgaagag gctcagcaaa acctgatcga tggttgttac cctatcgaaa
      361 aagaaaaaca aattacaact tatctcgata aagggaaaat gcaattatcc atatctgcac
      421 ctcaggcatg gttaaaatac aaagatgcaa actggacgcc tcctgaactt tgggatcatg
      481 gtattgctgg ggcatttctt gactacaatt tatatgcctc tcattatgca ccacatcagg
      541 gcgataattc acaaaatata agttcctatg ggcaggctgg ggttaatctt ggggcctggc
      601 gcctgcgtac tgattaccag tacgatcagt catttaacaa tggcaaaagc caggcgaaca
      661 acctggattt tccgcgtatt tatttgtttc gcccaatccc agcaattaat gcaaaactaa
      721 ctataggtca atacgatact gaatcctcta ttttcgactc tttccatttt tctggcgttt
      781 cgttgaaaag cgatgagaat atgttgccgc ctgatctacg tggttacgca ccgcaaatca
      841 cgggtgttgc acaaacgaat gcaaaggtca ctgtctcaca gaacaaccgt attatttatc
      901 aagaaaatgt tcctcctggc ccatttgcta ttactaattt attcaataca ttacaggggc
      961 aactggacgt caaggttgaa gaagaggacg ggcaagtcac gcaatggcaa gttgcatcta
     1021 atagtattcc ttatctgacg cgtaaagggc agattcgcta caccactgct atgggtaaac
     1081 cgaccagcgt tggtggtgat tccttacaac aacccttctt ctggactggt gaattctcat
     1141 ggggttggct gaacaatgta tccctgtatg gtagttcagt tttaacaaac cgtgattatc
     1201 aatctctggc tgccggcgtt ggctttaacc ttaactcatt gggttcatta tcttttgatg
     1261 tcacacgatc tgatgctcag ttgcataatc aggataaaga aacgggttat agctaccgcg
     1321 ctaactattc aaaacgtttt gaatctaccg gtagccagct cactttcgct ggttaccgtt
     1381 tctctgataa aaactttgtg acaatgaatg aatatatcaa tgacactaac cattacacga
     1441 attatcagaa tgaaaaagag agttatattg tcacgtttaa ccagtatctt gaatcattaa
     1501 ggttaaatac atacgtaagt ttggctcgta atacttactg ggacgccagc agtaatgtga
     1561 attattcatt atcacttagc cgcgattttg atatcgggcc attaaaaaac gtctccactt
     1621 ctctaacatt tagccgaata aactgggaag aagacaacca ggatcaactg tacctaaata
     1681 tttcgattcc ctggggaacc agtagaacat tgagctatgg tatgcaacga aatcaggata
     1741 acaagatttc gcatactgct tcgtggtatg actcttccga tcgaaataat tcctggagcg
     1801 tttctgcttc aggcgacaat gatgaattca aagatatgaa agcgtcacta cgcgccagtt
     1861 atcagcataa taccgagaac ggtcgtctct atctctccgg gacatcacag cgagacagtt
     1921 attattctct gaatgccagt tggaatggtt cattcacggc gactcgtcac ggagccgctt
     1981 tccatgacta tagtggtagt gctgactcgc gttttatgat cgacgcagat ggtgcagaag
     2041 atattccgtt gaacaataaa cgcgcggtaa ctaatcggta tggcatcgga gttattccat
     2101 cagtcagcag ttacataaca acatcattaa gcgttgatac ccgaaatctt ccagaaaatg
     2161 tggatatcga aaactcggtt atcaccacca ccttaaccga aggtgctatt ggctacgcca
     2221 aacttgatac ccgcaagggc tatcaaatca tgggggttat tcgcctggca gatggtagtc
     2281 acccaccact ggggattagc gtaaaagata aaaccagcca taaagaatta ggactggttg
     2341 ctgatggcgg ctttgtatac ctcaacggca ttcaggatga cagcaaactt actttacgct
     2401 ggggtgataa atcctgtttt attcaaccac ccaatagcag caacttaacc accggaacgg
     2461 ttattttacc gtgtattagc caaaattaat tcaggatgaa actaatgcgc tacttaaaca
     2521 caaagaatct cattgcagca ggagtattac tcgcctgcat gagcagcatt gcctggggtg
     2581 ccatcatccc ggaccgtacc cgtatcataa tgaatgagtc agataaaggg gaagcattaa
     2641 aacttactaa tcaaagcaaa aatctaccct atctggcgca aacatggata gaagatacga
     2701 aaggcaataa atcacgtgac tttattgtca cggttccccc tatggtacgt ttaaatccca
     2761 gtgagcaaat tcaaataaga atgataagtc aggaaaaaat tgctcaactg cctaatgaca
     2821 gagaaacgct cttctatttt aacgtgcgag aaataccgcc aaaaacagat aaaaaaaatg
     2881 tgatgcaagt cactatgcag cacgcattga aactattctg gcgaccaaaa gctattgaat
     2941 tagaagatga tggggtaatg tcttacgaaa aagttgaaat catccgtaga aatgatggtt
     3001 cgatccgctt taacaataag atgccttatc acgttacgct gggttatatc ggaacaaacg
     3061 gaatcacaat gttgccacaa acgcaaagtt taatggtaac cccttatagc catgcagata
     3121 cgcagtttaa aaatgttccg tcagcttttc aggttggtta tatcaatgat tttggtggat
     3181 taagttttta tgaaataaac tgcccgacgg ttaataactc ttgtaatgtt tccgtagcca
     3241 aacgagataa ataatatgcg ctacttatta attgtaatta tatttattat aggttttagt
     3301 gcattacctg tatgggcaat ggactgctat gctgaacatg aagggggaaa taccgtagtc
     3361 attggttacg taccaagaat ttctatcccc agtaatggta aaaaaggtga taagatctgg
     3421 caaagcagtg agtattttat gaatgtcttc tgtaataatg cattacctgc cccatcacct
     3481 ggagaagaat acccatcagc ctggactaat ataatgatgt ttttaccagg cggtcaggac
     3541 ttttataatc agaattccta tatttttggc gtaacataca atggagttga ttatgattcg
     3601 actgccccta atgctatcgc agcaccagaa tgcattgata taaaaggagc gggtaccttt
     3661 aataatcact ataaaaaccc cgctgtctgt agtggaggtc ctgagcctca attatcagta
     3721 acctttccag ctcgcgtaca actctatatt aaattagcaa aaaatgccaa tagagttaat
     3781 aaaaacctgg tgttacctga tgaatatatc gctctagagt ttaaaggtat gagtggcgca
     3841 ggagctatag aagtagacaa aaatttgacc ttcaggattc gtggattaaa taatattcat
     3901 gtccttgact gctttgttaa tgtcgcactg gaaccagctg atggcgttgt tgactttggt
     3961 aaaataaatt cccgaacaat taaaaatacc agcgttagtg agacgtttag tgtagtcatg
     4021 acaaaagatc ctggtgcggc ctgtactgag caattcaata ttttagggag ctttttcact
     4081 acggatattt tgagtgatta tagccatctg gatatgggta atggactgct attgaagata
     4141 tttcataagg atggaacagc aacggaattt aaccgcttct cacaatttgc ttctttttca
     4201 tcgtctagtg caccttcggt caccgcacca ttcagggcag aacttagtgc gaatccggca
     4261 gagactgttg ttgagggacc gtttagtaaa gacgtcatcc tcaaaatcac ctataactag
     4321 catctaatac aaacactaaa acgggccatc aggcccgtta tatcagtgct ctaactccag
     4381 ctctcttgcc tgcatcatct gttgacaata ccaggcataa cgtgcgcaaa accaggttct
     4441 atgttcttca tccatgttac cagtaacggc aagaatatcg accgggcgac cttctgcgtg
     4501 cattctggca aaactacgcg ccaggaaatc gaaattattt aaagaattaa gactatgatc
     4561 cataagcaca tcctccggtt atcgcatacc ttttaaggcc atatgctttt tctgatttca
     4621 gtattcccca atcagtgtag tgcctttaaa cataacgtgc gtaaatatat tgtccccgat
     4681 cacacttt
//
LOCUS       DBANUU010000238         4637 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000238 DBANUU010000000
VERSION     DBANUU010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.242"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..467
                     /locus_tag="ACIE68_004567"
     CDS             <1..467
                     /locus_tag="ACIE68_004567"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910749.1"
                     /translation="KLTFSHITAYTRNSTQQTAPQRPIFTTDDPTHYQQKHTLKIVPV
                     ALHNHINNLFSGITKQLNNTYAIPKTTNLFMAGYRKDPPLLPRLPDNQPNFHRQSENI
                     HTRDTHDTPLPKQRRNIRITETPTKPVTARFLSIPKDTKHNNHGKRHYPIHE"
     gene            1372..1701
                     /locus_tag="ACIE68_004568"
     CDS             1372..1701
                     /locus_tag="ACIE68_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910750.1"
                     /translation="MFANIDINQIKKLTQKEFDQFYELEGWSSTLINSRWVLELMTRD
                     DAPALMICDMGEDADFMDMSEFCVDTYNRSQKYYFTCDSENDVISKVYLHLVQHWDVQ
                     EFLEVFE"
     gene            1855..2166
                     /locus_tag="ACIE68_004569"
     CDS             1855..2166
                     /locus_tag="ACIE68_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910751.1"
                     /translation="MKKSLVLGLDKDQKRKEKPALVAQLTLLDIVANGTSIRLFRETA
                     VSFDKNTFTRYVMNVRRQRGKGWVAFQRMWPEHQLELALMEVNRVAQQEIQRASVMAI
                     A"
     gene            2293..2688
                     /locus_tag="ACIE68_004570"
     CDS             2293..2688
                     /locus_tag="ACIE68_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910752.1"
                     /translation="MTVKREKLTVDVYYASETAEGKNVAKITVVTYNTETGAEVQAST
                     IVRKGDASGGEYATQYQSILDATDPLLLKIENYFRQVDEEVFETMMNMVNTVFASSLN
                     TSTTWIGQYGLRITSGIPADTLIPESVFA"
     gene            2806..3180
                     /locus_tag="ACIE68_004571"
     CDS             2806..3180
                     /locus_tag="ACIE68_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910753.1"
                     /translation="MGLDIYIETQPKNDLNNEASRKQVAYFRKFNALVGWMERNVGEV
                     ENCELLELTMNDICLLKAHLMHINESNCEEYLPTREGFFFGSQEYDEGYWHDVDKLKK
                     LVEDLIRNHDFHNNRLTFCAWW"
     gene            3531..4013
                     /locus_tag="ACIE68_004572"
     CDS             3531..4013
                     /locus_tag="ACIE68_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001711142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910754.1"
                     /translation="MNFIATVNAPAHGNIAVTFSDIEKRVLGAWRDNETVELSAQEKC
                     IIARDIIGNRRYSRVFEKAYVVNSGFGTFVFPVRSGRFCQSKLIEFATQISVWIKTQS
                     SFKFSDDEAVSQGMRIANNAIKCKNITYTAGVDTWKLFCANFMLNVYASNRIHILDGV
                     "
BASE COUNT         1421 a         1077 c         1033 g         1106 t
ORIGIN      
        1 aaaaattgac gttttcacac atcactgctt atacccgtaa ttctacgcag cagacagcgc
       61 cacagcgtcc aattttcacc acagacgacc caacacacta ccaacaaaaa cacacgctca
      121 aaatcgttcc cgttgcgtta cataaccata taaacaactt attttcaggc ataacaaaac
      181 aactaaataa cacatatgcc attcccaaaa caaccaacct ctttatggca ggctaccgaa
      241 aagacccacc tcttcttccc aggctaccgg acaaccaacc taactttcac aggcaatccg
      301 aaaacattca tacgcgcgat acccacgaca cgccattacc caaacagcga agaaacatca
      361 gaatcactga aaccccaacg aagcctgtaa ccgcaaggtt tctctcaatc ccaaaagaca
      421 cgaaacataa caatcacggg aaacgccatt acccaataca cgaataacta caaaagccct
      481 tcacgcactc cagacaaaca actcaaacac aataacgaaa ggaatcgccc acctgccatt
      541 actccataca cggagaagaa caatcaccaa aacgacgaga acaccccata gaagaaccac
      601 cgcaaaacga acgaaaccat ttacaccgac agagagaaca acacgcccaa ttcaccaaca
      661 gaaatagtcg ccgtatagaa cgttctggaa ggagtgagca ttaacgccta tagagaggtt
      721 gagacagata aaacacgaag aaaacacaac atgtatagcg aagaagccag gtatgtataa
      781 gtggggaggg aaggaggggt gtcgcctcct ttttcgtatt tattcaactc ctgattttat
      841 cccccgtaac acccctacgg tcagccttcg gtccatacag ggaaaaggtt gcatcccgct
      901 acagaaacgg ctgggttgcc ttcggggaac ggctggaggt tttcagggaa acggtcgagt
      961 tgcctgtgtg gatttcggga aaaggctcga tgccggttca ggaacggcgg cgtaccggta
     1021 tagcgtgagg gagagttgcg gctacccacc tgtaatgtgc gggaagaaga gaggcgctca
     1081 cgtgcaggcg aacttactta ccgctggatt ccctctgaat gcccagctac agcgtaattg
     1141 tgccagacag ccaacgggtc acaatcgatt tcagtaggtt ttcggggaga ggattattct
     1201 gcccaccagc caccttcggc cactaaatca gaataatgta aaaagtgtac tcgatactga
     1261 acctgaaaac tcactgccaa aacaacattc accggaataa aatccttttt cgcctgtgcg
     1321 ttattgcgat aataacacca acgagaaaac aagttgttta aggattgctt tatgtttgca
     1381 aatatcgaca tcaaccaaat caagaaatta actcaaaaag agtttgatca gttttatgag
     1441 ttagaaggtt ggtcttccac tctgatcaat tcgcggtggg tgcttgagct gatgactcgt
     1501 gatgacgcac ctgctttgat gatttgcgac atgggtgaag atgctgactt tatggatatg
     1561 agcgaatttt gtgtggacac atacaaccgc agccagaagt actacttcac atgcgatagc
     1621 gaaaatgacg tgatttctaa ggtctatctt cacctcgtcc agcattggga cgttcaggag
     1681 tttcttgaag tattcgagta accccaacca aagccagcaa tgctggctta atttctctat
     1741 agccgcaggg aggaacctgc atatcgatca tgcacctgat cattatgctt tgtagccgtt
     1801 ttcttactgg tattatttac gccattgaga aaacaagttg tttacggagt tgtgatgaaa
     1861 aaatcattgg ttcttggtct ggacaaagac cagaagagaa aagagaagcc tgcactggtt
     1921 gctcaattaa ctttgctaga catcgttgcc aatggaactt ctattcgtct gttccgtgaa
     1981 acagcggtgt ctttcgataa aaacaccttt actcgttatg taatgaatgt tcgtcgccag
     2041 cgtggaaaag gatgggtggc atttcaaaga atgtggccgg aacatcagct cgaactggcc
     2101 ttgatggaag ttaaccgcgt ggcccagcaa gaaattcaga gagcatcagt gatggcaata
     2161 gcctgataat gtgcaagtgg taattagtcg acagtacgac agccccgcca tccttacggg
     2221 gcttttttgt attgtaagta catacttacg atcatagaat ataaaaataa ccaatacact
     2281 acggagagtg aaatgaccgt taaacgcgaa aaactgacag ttgatgttta ctatgcctct
     2341 gaaaccgccg aaggtaagaa tgtggcaaaa atcaccgttg ttacgtacaa caccgaaact
     2401 ggtgccgaag tccaggccag tacgatcgtg cgtaaaggtg acgcctccgg cggcgagtac
     2461 gcgactcaat accagtccat tctcgatgca actgacccgc tgctgctgaa aatcgagaac
     2521 tacttccgcc aggttgatga agaggtgttt gaaaccatga tgaatatggt taacaccgta
     2581 ttcgcctcca gcctgaacac cagcaccacc tggattggcc agtacggtct gcgcattacc
     2641 tctggcattc ctgccgacac cttaatccct gaaagcgtat tcgcttaatc ctctttaaat
     2701 ggcgcgtaaa ccgcgccatt ttattatgcc cgataacaat ttgttttctg ccttatcaga
     2761 tttgtgaaaa tgatttcact gaagcaactt aataaggaaa ccatcatggg acttgatatc
     2821 tatatcgaga cacagcctaa aaacgatctg aataacgagg catccagaaa gcaggttgct
     2881 tacttccgta agttcaatgc gctcgttggg tggatggagc gcaacgtagg tgaagtcgaa
     2941 aattgtgagc ttttagaatt aacgatgaat gacatttgtc ttctgaaagc tcatttgatg
     3001 cacataaacg aaagcaattg cgaagagtac ttgcctactc gggaaggttt tttcttcggc
     3061 agtcaggagt acgacgaagg ttactggcat gacgtggata agctgaaaaa acttgtggaa
     3121 gacctgatca ggaaccacga ctttcacaat aacagactga ccttctgcgc ctggtggtaa
     3181 atatgggcga tttcaagaaa cgcctgaagg aaagagccga gatggtcaga aagcaaaacg
     3241 cctcttctgt catcagatac gcaaggcagt ttagtcgcaa caacaaatca gttgaggaaa
     3301 agatccttag cgtaatcggt cgataattat taaggccatc agcattgatg gccttaaatg
     3361 accatcctgt ttcccgcagg ctaaaaacac caacctctta cctccaggca accgacaaac
     3421 ccacctgttc ccgtccggct accgcaactt tccactttga cgccttattc gtacaacgat
     3481 aattaacacc aataagaaaa caatttgtta tttacgataa ggaattaatc atgaatttta
     3541 tcgctactgt aaacgcaccc gcacatggca atatcgctgt aacgttctct gacattgaaa
     3601 aacgagtact tggtgcatgg cgcgacaatg agacggtgga actgtcagca caagaaaaat
     3661 gcattattgc acgcgacatc attggcaatc gtcgttactc gcgggtattt gagaaagcgt
     3721 atgtggtaaa ttctggattc ggaacgttcg tctttccggt gcgctccggg cgattctgcc
     3781 agtccaagct gattgagttc gctacgcaga tttctgtctg gattaaaact caatcgtcgt
     3841 tcaaattttc cgacgatgaa gcagtatcgc aggggatgcg gatcgccaac aatgcaatta
     3901 aatgcaaaaa cattacgtat accgctggcg ttgatacatg gaaactgttt tgcgctaact
     3961 ttatgctgaa tgtatacgca agcaaccgca tccacatcct tgatggcgtg taactgagaa
     4021 gagggccaga aacggccctt tctctatagc caccagctgc cgcagggaaa ttttcagaaa
     4081 cggcgaggaa cgtattcatg agccgacggg aaacggccag gattttttcg ggaaacggct
     4141 gcattcgcct ttatgtagaa aaaacatcgg gaagctggtg gaatccaacc agcggttgtc
     4201 ggacaggtga gcggggaaaa ttatgatgac tttcgtcgcc tgagacatcc agatttcttt
     4261 cgtagcgtaa tcacatacgt gattaagtgg tgtgattatg tgaaaaatca cgcgcacata
     4321 atacgcgagc ggacacagaa caacacaaaa tgccgatccg cgccgacaaa taaacgcgga
     4381 tcacatagca agactaaaag ccaatgatta accatacgct atagcgcaat ataacgcgtt
     4441 ttaagcgcgt taatgtgtta agtaatgggt atgtactggc aaggatataa aagcgcgtct
     4501 atggcgttat tttggcgctt atttttatgt tgttggagtg agttaaagac aataaaaaac
     4561 gcgccatcat tggcgcgtta tggtgggagt attggaaacg aaaaaagcgc ccatagtggg
     4621 cgctcgattt tatttgt
//
LOCUS       DBANUU010000239         4597 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000239 DBANUU010000000
VERSION     DBANUU010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.243"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            219..2369
                     /gene="btsT"
                     /locus_tag="ACIE68_004573"
     CDS             219..2369
                     /gene="btsT"
                     /locus_tag="ACIE68_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFX5910755.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTTQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            2419..2622
                     /locus_tag="ACIE68_004574"
     CDS             2419..2622
                     /locus_tag="ACIE68_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFX5910756.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            2633..3589
                     /gene="yjiA"
                     /locus_tag="ACIE68_004575"
     CDS             2633..3589
                     /gene="yjiA"
                     /locus_tag="ACIE68_004575"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFX5910757.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIT
                     DKQNNISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(3623..3925)
                     /locus_tag="ACIE68_004576"
     CDS             complement(3623..3925)
                     /locus_tag="ACIE68_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HFX5910758.1"
                     /translation="MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(3912..4188)
                     /locus_tag="ACIE68_004577"
                     /pseudo
     CDS             complement(3912..4188)
                     /locus_tag="ACIE68_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
     gene            complement(4387..>4597)
                     /locus_tag="ACIE68_004578"
     CDS             complement(4387..>4597)
                     /locus_tag="ACIE68_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFX5910759.1"
                     /translation="MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRK
                     SLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         1017 a         1238 c         1174 g         1168 t
ORIGIN      
        1 atcacatatt tatcgtcact taaacgacgc ctttgccgct caaccgcaaa actgaccgct
       61 tacatcccta aaataaccac tcagttattt accttacttt acgcgcgcgt aactctggca
      121 acatcactac aggatagcgg tcaatttacc tcctcaaacg caacgcaaac ctagaacggc
      181 ttcggccaac tattaatcaa tacatgccag gttttactat ggatactaaa aagatattca
      241 agcacatacc ctgggtgatt ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat
      301 tacgtcgggg ggagcacgtc agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc
      361 tggtggcgta tcgctactac agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca
      421 cgcgcgcgac gcctgcggtt ataaacaacg acggtctgaa ctacgttccg accaaccgtt
      481 acgtgttgtt tggtcaccac ttcgccgcta tcgccggtgc tggtccgctg gtgggtccgg
      541 ttctcgccgc gcagatgggc tacctgcctg gcacgctgtg gctgctggcg ggggtcgtgc
      601 tggccggtgc ggttcaggac tttatggtgc tgtttatctc ctctcgccgt aatggcgcat
      661 ctcttggtga gatgatcaaa gaagagatgg gaccagtacc ggggactatc gcgctgtttg
      721 gctgtttctt aatcatgatc atcatcctcg ccgtcctggc gctgattgtg gttaaagccc
      781 tggccgaaag tccgtggggt gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta
      841 tgggtatcta catgcgcttt attcgtccgg ggcgtgtggg tgaagtctct gtcattggta
      901 tcgtgctgct ggttgcctct atctacttcg gtggcgtgat tgctcacgat ccgtactggg
      961 gtccggcact gacctttaaa gacaccacca ttaccttcgc gctgattggc tatgcgtttg
     1021 tttccgcact gctgccagtg tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc
     1081 tgaaaatcgg cgttatcgtc ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga
     1141 aaatgcctgc catgacccag tacattgacg gtactggccc gctgtggaaa ggcgctctgt
     1201 tcccgttcct gttcatcacc atcgcctgtg gtgcggtatc tggcttccac gcgctgatct
     1261 cttccggtac gacgccaaaa ctgctggcta atgaaaccga cgcgcgtttc atcggctacg
     1321 gcgcaatgct gatggagtcc ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg
     1381 aaccgggtct ttacttcgcg atgaacaccc cgcctgctgg ccttggcatc accatgccta
     1441 acctgcatga aatggggggc gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta
     1501 ccgcacacgc ggcagcgacc gtcagctcct ggggcttcgt gatttcgcca gagcagatcc
     1561 tgcaaaccgc gaaagacatt ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccga
     1621 cgctggcggt aggtatcgct cacgtgttcc acaaagtgct gccgatggct gacatgggct
     1681 tctggtatca cttcggtatt ctgttcgaag cactgttcat cctgaccgcg ctggatgcgg
     1741 gtacccgttc tggccgcttt atgctgcaag acctgctggg taacttcatc ccgttcctga
     1801 aaaaaaccga ctctctggtt gccggtatca tcggtactgc gggctgtgtg ggtctgtggg
     1861 gctacctgct gtatcagggc gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc
     1921 tgttcggtat ttccaaccag atgctggcag ccgtagcgct ggtactgggc accgttgtgc
     1981 tgattaagat gaagcgcacc caatacatct gggtaactgt tgttccggct gtatggctgc
     2041 ttatctgcac cacctgggcg ctgggcctga aactgttcag caccaacccg cagatggaag
     2101 gcttcttcta catggcaagc cagtacaaag agaagattgc taacggtact gacctgacga
     2161 cgcagcagat tgccaacatg aaccacatcg ttgtgaacaa ctacaccaac gcaggtctga
     2221 gtattctgtt cctgattgtg gtgtacagca tcatcttcta cggtttcaaa acctggcttg
     2281 cggtgcgtaa cagcgacaaa cgtactgaca aagaaacacc gtacgttcca atcccggaag
     2341 gcggcgtgaa gatctcttcg caccactaac cgtgtttagc cccgcttcgg cggggctttg
     2401 ttcttatcag agtgaactat gtttggtaac ttaggacagg caaaaaaata tctcggccag
     2461 gcggcgaaga tgttgattgg cattccagac tacgacaact acgtcgagca tatgaagacc
     2521 aaccatcccg acaagccgta catgagctat gaagaattct tccgcgaacg ccagaacgca
     2581 cgctacggcg gcgatggtaa aggcggtatg cgctgttgtt aatggagaga gcatgaaccc
     2641 gattgcagtt accctactca ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca
     2701 tattcttaac gaacaacacg gctacaagat tgccgtgatt gaaaacgaat tcggcgaagt
     2761 ctccgttgat gatcaactga ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg
     2821 ctgcatctgc tgttcgcgct ccaacgagct ggaagacgca ctactcgacc tgctggataa
     2881 tctcgacaag ggcaatattc agttcgaccg tctggtcatt gaatgcaccg gcatggccga
     2941 tcccggcccg attattcaga cctttttctc ccatgagatt ttatgccagc gctacctgct
     3001 ggacggcgtg attgcgctgg tggatgcggt acatgccgat gagcagatga accagttcac
     3061 catcgcccag tcccaggttg gctacgccga ccgtattctg ctgacgaaaa ccgacgtcgc
     3121 aggcgaagca gagaaactgc gtgaacgcct ggcgcgcatc aacgcccgcg caccggtcta
     3181 caccgttacc cacggcgata tcgacctggg ccttctgttc aacaccaacg gttttatgct
     3241 ggaagaaaac gtcgtcagca ccaaaccgcg tttccacttt atcacggaca aacaaaacaa
     3301 tatttcgtcg attgtggtgg aactggatta cccggtagat atcagcgaag tttcccgggt
     3361 gatggaaaac ctgctgctgg agtcggcgga taaactactg cgttacaaag ggatgctatg
     3421 gattgacggc gaacctaacc gcctgctatt ccagggcgtc caacgcctct acagcgccga
     3481 ctgggaccgc ccgtggggcg atgaaaaacc gcatagcacg atggtgttta tcgggattca
     3541 gttgccggag gaagagattc gggcagcgtt tgcggggttg aggaagtaat tttggatgat
     3601 ggccgccatg tggcggcctg aattattttt tgttctgatc gcgcagcatc gcttcacgta
     3661 aaattgcgtt caggcgagtt tgatagcctt tacctggtcg cttcaaccat tccatcacat
     3721 cagcatcaat acgcaccgaa gcctgagttt tcaatggccg ataaaactta ccgcgttcgg
     3781 cgttagacca ttgctcatcg ctggaaggtg ggatatcact gtaatcaata tcttcgtcgg
     3841 atttattcgc cagcgccttc agttcggcct catgctgcac atcgagtgta gaggaggtcc
     3901 ctcgtttatg tttaaccatg ctcatagcga ttcctctctt tacgatctgc ttttcgtgca
     3961 ctgatgattc gaataatttc atttccgctt tcgaaacgga tggtatgcgc aaccagaata
     4021 accacgatgc cgtaatgtct gccagcgata ctcaccgttt tctatgcgtt cttgctgtga
     4081 caaatgttgt ggatcatcaa acaataacac cgcatcttca aagcggatgc catgtttcac
     4141 ccgattactt ttggccttgt tttcatccca ttcaaactcc attggcataa ttgccatccc
     4201 taattgtata tacaaaaatg tatctacaat tattctgcaa ttatagcgtt gatgttggaa
     4261 atagcatcac gtttcgaata atcaaccatg aaattgacaa tgagtaagtt ggacttgatt
     4321 catccaatag aatttctcgc ctgacatggc acactaaaca cctcgctgtc gggtaatgac
     4381 tccaacttac tgatagtgtt ttatgttcag ataatgcccg atgaccttgt catgcagctc
     4441 caccgatttt gagaacgaca gtgacttccg tcccagcctt gccagatgtt gtctcagatt
     4501 cagattatgt cgctcaatgc gctgagtgta acgcttgctg ataacgtgca gctttccctt
     4561 caggcgtgat tcatacagcg gccagccatc cgtcatc
//
LOCUS       DBANUU010000240         4571 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000240 DBANUU010000000
VERSION     DBANUU010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.244"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(496..3751)
                     /locus_tag="ACIE68_004579"
                     /pseudo
     CDS             complement(496..3751)
                     /locus_tag="ACIE68_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
     gene            complement(3816..3935)
                     /locus_tag="ACIE68_004580"
     CDS             complement(3816..3935)
                     /locus_tag="ACIE68_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFX5910760.1"
                     /translation="MLLSYVEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(4159..4317)
                     /locus_tag="ACIE68_004581"
                     /pseudo
     CDS             complement(4159..4317)
                     /locus_tag="ACIE68_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         1151 a         1188 c          929 g         1303 t
ORIGIN      
        1 cctgtgtgat tttcagtacg ggggattaat caccggtatg agtcacacca gcagaatgcc
       61 ggaggtgaag aatcgaatgt tcacccttca cccattattc accacctatc atactgaaat
      121 aaaaggagaa aaaagaaagg tgaacagtgt gaacagttct ttcgaaaaaa aatttttttc
      181 ctgtacgata tagcatcaat ccaagtaaaa atgcgcttta atttcacatt aacttacagc
      241 cattggtata caattaggta tacgcacaaa aactgaatta aacaaaatcg ttaaaaatca
      301 aagcaatata atatttattc aaactccgcc agccaatcat gattggacgg tataaggaca
      361 acaccaataa aaacaggaag ttagcagtct cagcaggaca ccgaccagac ggtgaagaga
      421 cataaaagga tacgcaaagg agccgcggct cctggtgaca tgaaagccca tagatgtggg
      481 ctttttcgtt gatggtcaga acgaccagtt cacaccagct accccgttcc acggggattc
      541 cacaccggca ccatggctat gccccacccc aagatgcccg cttaacgtac tgctgaatga
      601 ggctttaata cctgcctggt atattccacg tctgcccgac aaatcattga cgaaattacc
      661 gtcactattc actttcaccc ggttatcatc gacaaattct ttgcgcacag ccgccttcag
      721 ccacggctca acttccatac cgttccccag acgcatgttg taactcagcg ttgcacccag
      781 ttcacgatat aaactgcggg tatcgactga tttcgattcc atgccattgg ataaatgata
      841 ttcagggtta tcagcggtga acccccgtta acgatgcata cggcgtcagg ttccagttac
      901 catcggtaaa tcgcatcccg gtttcaatgt gaccgcccag cccgtagctg cgataactgc
      961 cgttggcggc tccaccgctg ccaaccgtgg agtcgcgagc ttcggtacct gctaatacca
     1021 ataaattacc gccattttcc agcaacatat tggtcgctaa attgccggaa atggaaaaag
     1081 tgccgtactg gtgagtaccg ctgatttcaa taccgttagc cgtgctcgtc tggagagcgg
     1141 caccgctgtt ctggacgata tctgtcgctt tgccattatc gttaactgtc agcgtaccgc
     1201 cttcattgat ctttgttttt attgcctctc cgttagctga aactgtttgt attccgccgt
     1261 cgttaatcgt tgtctcattc gccaccccct cgacgatttg ttcgccgccg gtgagcgtcg
     1321 tgcctgtcgc agtggctttt gttttgacga tctcccgtcc gcccgtattg acctgtgttt
     1381 tgtcagaaga ggtgtctgac tccacggtta acacgccgcc atttgccagc aggatattgt
     1441 tcgccgcacc ctgctcgatg ctgaacgcga cgccatccgc gcgtgttcct gtgacccgcg
     1501 tcgccctggt ggttgcaacc aaagcgccct ggctactctg ctgtatcccc gttgcgctgc
     1561 ctttctcccg cacatcgagt gtgccgccgt cattaagcac cgagttttca gccagaccgc
     1621 cctcattaac tacctgtgaa cacccattaa taatggaacc ttccgctatc ccgtttgcca
     1681 taatttgttg taggccagag acgatatcgg tattgatcgc cttaccataa ttctgaacgg
     1741 tttgtgtgcc gccattgatg tgcgttttgt cagttgaccc accatcaaca atttgttcac
     1801 cactttcaat atttgcctca gtggctaaac catataccgt ttgcttgcca cctttgatat
     1861 ttgctttatc agaagtggca ctggcatata ttgtttgggt gccagcacta ttaagtacag
     1921 tgccaacatc ttttccataa acatccattt tgccgttggc attaataatc gtatcaactg
     1981 cccgggaacc agtgacgact gttaatgagc cagcgttttc cagcactaca tttttagctt
     2041 ctgaattcct gatgtagaaa gcatcaccat aactgttggt tccttcgata agggttccgg
     2101 aagttgtgga agcaattaat gcgccgccgg attgttgctc aacatgctta gcctcaccac
     2161 cgtcctgaac ctccagaacg ccaccattat taagtctggt ggtatctgtt ttagcctcct
     2221 tctggacaat cagcttaccg ccagcatcaa cggtagtatt tttcgccgag gttttagcca
     2281 ctaccgtcag ttcgccggta ttttccagca caacataatt agcctcccct ccggtaatag
     2341 tgaagtgaga gagcttgttg tatccttcaa tatcagtccc tgcgcccgtg ttggcaacta
     2401 aagcactgcc cgtctcctgg ttaaccccat gtgcaatacc gccggtatag acaatcagcg
     2461 agcctccggc gctaatattg ctgccaattg ccgtaccatc tttctcaaca acttgctggc
     2521 tcccggagga tataattgtt gtgtcagctt tcccaccgct tttgatattt tgcgttccgc
     2581 tgttgatatt ggtgcctgtg gctatgccat aattattaag attctgtgtg ccaccattaa
     2641 ttatggtatt tgtcgcgttt cctgcaacat ccataacccc gccattatct attcgggtcg
     2701 catcagcttt agcattcgtt aaaactgaca ttgttccttt atctttaata atcgtcttgt
     2761 ttgcggaatg agatacttct aaatggcctc cattttccag caacacattg tcttccactt
     2821 tattgtggat ggagaatgca ccttcactat tcgtaccgct caccgtcgta ccgttagtgt
     2881 ttgtttttaa aattgcacca tcgtgctggg taacatttgt tgccgtacca ccactaacat
     2941 caagcacgcc accggaataa acttcaataa catccgaggt gctggtgtta tcaacaattt
     3001 gcgtgccacc agaatagatc tgcgtaattt ttgccgttga cttactattc agagactgag
     3061 ttccgccttc aatcgtcgtg tccagcgcac ggctctcata tactctttgc tcaccgccat
     3121 ttttaatggt tgttgtttct actgtgctct gttcaacata ctgtcgacca ccatttatgg
     3181 ttgtgttcgt tgccagactt ccttgtacta cgtcctgaga gccagactta tttatcgttg
     3241 taccatcagc atgcccttga acttttacta tctggctacc accatcaatg agtattccat
     3301 tcgcactccc tccctctacg cgtgaagcac cgcccttaat tgtcgttcca ttgctgatac
     3361 cccctttata aacgtcctga ttgccactct cgattgtcgt acctgtggaa atacccccgt
     3421 catgaattga ctgtctgcca ccgttaatgg ttgtattatt agcctgcccc acaaaattgt
     3481 tatgacttcc tatatcttga tatccaccag attcaataag acttccatta gataccccgc
     3541 catgaacatt ctgctggtca tggttgataa tatgagtgtt atttgttgta cctcgttcat
     3601 ccactttttg gttgccatct acagtctcat cgtttaccac accaataaca tcaggagtga
     3661 aggccgccat accgggcggg gcatatatca aggcagatat caataaggaa agtactgagc
     3721 ggcgacaata atggggactg gtcctgttca taaatttcat cctctgaaaa gtgaatactg
     3781 agtagcgttt aagcgacctt agctctgctg caacatcagc ccacaggcac cagaccaggg
     3841 gattcatcct gaagagacag cgcaagtgta ttgtgttcac cgctcatcaa agacatcatg
     3901 atgaaatgat gatattccac ataagaaaga agcatttttt aaacgcagtg cgctgaagta
     3961 tggttggata aaaaagtcaa tctattcagg gaatacgggg gtattctttt ctttcgacaa
     4021 tcaggtcgtc ggcaaaataa aatgatttac ataatcgttt ctgatgaata tcttctgctc
     4081 acataaaaat cacacaataa ctttgagatc gcagattctt ttacttttac agcattcgtc
     4141 cccccattgt tgggcaaata tagattgggc cagagcacga aagttaatac cacgtttgca
     4201 cagctcctcc aacagcacga caagatgcca catactgcgc cccagtcggt tcggtttaca
     4261 gactacccgt gcgtccaccg ccgataatgt cctgagcagt tttttcagtc cggacctcct
     4321 gccacggttt tacgttgaag aggtaaccct gagcacgcag ttcatcagtc aggcgtggtg
     4381 taccgtaacc ctattattga tgggtaagat caaaaaaact ttcaggcagc taaggaaagt
     4441 tgaaccagac attagaataa atatttcaac caattacagc accaattcag acaccgccag
     4501 ctcaacaaat aaatcaaggg gttacgtgaa agcgtagccc ctttttcttt ggtagtggca
     4561 gcaaaatggt t
//
LOCUS       DBANUU010000241         4435 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000241 DBANUU010000000
VERSION     DBANUU010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.246"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            71..613
                     /gene="fimA"
                     /locus_tag="ACIE68_004582"
     CDS             71..613
                     /gene="fimA"
                     /locus_tag="ACIE68_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFX5910761.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            833..1525
                     /gene="fimC"
                     /locus_tag="ACIE68_004583"
     CDS             833..1525
                     /gene="fimC"
                     /locus_tag="ACIE68_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFX5910762.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            1556..4164
                     /locus_tag="ACIE68_004584"
                     /pseudo
     CDS             1556..4164
                     /locus_tag="ACIE68_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            4177..>4435
                     /locus_tag="ACIE68_004585"
     CDS             4177..>4435
                     /locus_tag="ACIE68_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5910763.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNF"
BASE COUNT         1185 a          961 c         1048 g         1241 t
ORIGIN      
        1 tcagtgtctt aaataaagta atcggttata tacggatgtg gagtcgataa atgagattga
       61 aggaatatat atgaaattaa gatttatttc gtctgcgctg gctgccgcac tattcgccgc
      121 tacgggtagt tatgctgccg ttgtagatgg cggtacaatt cactttgaag gcgaactggt
      181 gaatgctgcc tgttcagtga atactgactc ggcagaccag gttgtcacac tcggtcaata
      241 tcgtaccgat attttcaatg ctgttggtaa tacctctgca ttaattccat tcaccattca
      301 gttgaacgac tgcgatcctg ttgttgccgc taatgctgcc gttgcatttt ctggtcaggc
      361 tgatgcaatc aatgataatt tattggccat tgcatccagt accaatacaa caacagcaac
      421 gggtgtcggt attgaaatac ttgataatac atccgcaatt ctcaaacctg atgggaatag
      481 cttctcaacc aaccagaact tgatccccgg gaccaacgtt cttcattttt ctgcacgtta
      541 taaaggcacc ggtacaagtg catcagcagg gcaagcaaat gctgacgcga cttttattat
      601 gagatatgaa taatcaaaac cacgttgttt tgaattatat atcacgtctt ataacaaagt
      661 aatgtaccgg ttgtctgaag cggtatggtg gcaatgtaaa tcgaaatcat gttcactttg
      721 tatcatgccg ctttattaaa tgaaaaggga atgatgtgtt gtaagaaacc aaagcaatca
      781 tttctttata ttccttattt ttgccgtcag gaatacacaa ggcgtattaa ctatgatgac
      841 taaaataaag ttattgatgc tcattatatt ttatttaatc atttcggcca gcgcccatgc
      901 tgccggaggg atcgcattag gtgccacgcg tattatttat cccgctgatg ctaaacagac
      961 tgcggtatgg attagaaata gccataccaa tgagcgcttt ctggtcaatt cgtggattga
     1021 aaacagcagc ggtgtaaaag aaaagtcatt catcattaca ccgccactgt ttgttagtga
     1081 acccaaaagc gaaaatacct tgcgtattat ttacaccggt ccaccgctgg cggcagatcg
     1141 tgagtctctg ttctggatga atgttaagac gatcccttcg gtagataaaa atgcattgaa
     1201 cggcaggaat gttttgcaac tggcgatttt atcgcgcatg aaattatttc tccgtccaat
     1261 tcaattacaa gaattacccg cagaagcgcc ggacacactc aagttttcgc gatccggtaa
     1321 ctatatcaat gttcataatc catcaccttt ttatgtcacc ctcgttaacc tgcaagtggg
     1381 cagccaaaag ttggggaatg ctatggcggc acccagagtt agttcacaaa ttcccttacc
     1441 ctcaggagtg cagggaaagc tgaaatttca gaccgttaat gattatggtt cagtaacccc
     1501 ggtcagagaa gtgaacttaa actaaccgaa tcatctgaca atatcagagc taattatgaa
     1561 aatacccact actacggata ttccgcagag gtatacctgg tgtctggccg gaatttgtta
     1621 ttcatctctt gccattttac cctccttttt aagctatgcg gaaagttatt tcaacccggc
     1681 atttttatta gagaatggca catccgttgc tgatttatcg cgctttgaga gaggtaatca
     1741 tcaacctgcg cgcgtgtatc gggtggatct ctggcgtaat gatgagttca ttggttcaca
     1801 ggatatcgta tttgaatcga caacagaaaa tacaggtgat aaatcaggtg ggttaatgcc
     1861 ctgttttaac caggtactcc ttgaacgaat tggccttaat agcagtgcat ttcccgagtt
     1921 agcccagcag caaaacaata aatgcatcaa tttactgaaa gctgtacctg atgccacaat
     1981 taactttgat tttgcagcga tgcgcctgaa catcactatt cctcagatag cgttgttgag
     2041 tagcgctcac ggttacattc cgcctgaaga gtgggatgaa ggtattcctg ctttactcct
     2101 gaattataat ttcaccggta acagaggtaa tggtaacgat agctattttt ttagtgagtt
     2161 cagcgggatt aatattggcc cgtggcgttt acgcaacaat ggttcctgga actattttcg
     2221 cggaaatgga tatcattcag aacagtggaa taatattggc acctgggtac agcgtgccat
     2281 tattccgctg aaaagtgaac tggtaatggg agacggcaat acaggaagtg atattttcga
     2341 tggtgttgga tttcgtggtg tacggcttta ttcttccgat aatatgtatc ctgatagcca
     2401 gcaagggttt gccccaacgg tacgtgggat tgcccgtacg gcggcccagc taacgattcg
     2461 gcaaaatggt tttattatct atcaaagcta tgtttccccc ggcgcttttg aaattacaga
     2521 tttgcacccg acatcttcaa atggcgatct ggacgtcacc atcgacgagc gcgatggtaa
     2581 tcagcagaat tacactatcc cgtattcaac agtgccaatt ttacaacgcg aagggcgttt
     2641 caaatttgac ctgacggcgg gcgattttcg tagcggtaat agtcagcaat catcgccttt
     2701 cttttttcag ggcacggcac tcggcggttt accacaggaa tttactgcct acggcgggac
     2761 gcaattatct gccaattaca ccgccttttt attagggctg gggcgcaacc tcgggaactg
     2821 gggggcagtg tcgctggatg tgacccatgc gcgcagtcag ttagccgacg acagtcgtca
     2881 tgagggggat tccattcgct tcctctatgc gaaatcgatg aacaccttcg gcaccaattt
     2941 tcagttaatg ggttaccgct attcgacaca aggtttttat acccttgatg atgttgcgta
     3001 tcgtcgaatg gaggggtacg aatatgatta cgattacgac ggtgagcatc gcgatgaacc
     3061 gataatcgtg aattaccaca atttacgctt tagccgtaaa gaccgtttgc agttaaatat
     3121 ttcacaatca cttaatgact ttggctcgct ttatatctct ggtacccatc aaaaatactg
     3181 gaatacttcg gattcagata cctggtatca ggtggggtat accagcagct gggttggcat
     3241 cagttattca ctctcatttt cgtggaatga atctgtaggg atccccgata acgaacgtat
     3301 tgtcggactt aatgtttcag tgcctttcaa tgttctgacc aaacgtcgct acacccggga
     3361 aaatgcgctc gaccgcgctt atgcctcctt taacgccaac cgtaacagca acgggcaaaa
     3421 tagctggctg gcaggtgtag gtgggacctt actggaaggc cacaacctga gttatcacgt
     3481 aagccagggt gatacctcga ataatgggta tacgggcagc gccacggcaa actggcaggc
     3541 cgcttacgct acgctggggg tcgggtataa ctacgaccgc gatcaacatg acgttaactg
     3601 gcagctgtct ggcggtgtgg tcgggcatga aaatggcata acgctgagcc agccttttgg
     3661 ggataccaat gttttgatta aagcgcctgg cgcaggcggt gtacgcattg aaaatcaaac
     3721 tggcatttta accgactggc gcggctatgc ggtgatgccg tatgccacgg tttatcggta
     3781 taaccgtatc gcgcttgata ccaatacgat ggggaattcc atcgatgttg aaaaaaatat
     3841 tagcagcgtt gtgccgacgc aaggcgcgtt ggttcgtgcc aattttgata cccgcatagg
     3901 cgtgcgggcg ctcattaccg ttacccaggg cggaaaaccg gtgccgtttg gatcactggt
     3961 acgggaaaac agtatcggaa taaccagtat gtgggtgatg acgggcaagt ttatttaagt
     4021 ggtgcgccat tgtctggtga attactggtt cagtggggag acggcgcgaa ctcacgctgc
     4081 attgcgcact atgtattgcc gaagcaaagc ttacagcaag ccgtcactgt tatttcggca
     4141 gtttgcacac atcctggctc ataaaggaaa ttatcaatga agataatctg cagattatta
     4201 ttggcgatgg catgtttgtg tctggcaaac atatcctggg ctactgtttg tgcaaatagt
     4261 actggcgtag cagaagatga acactatgat ctctcaaata tctttaatag caccaataac
     4321 cagccagggc agattgttgt tttaccggaa aaatccggct gggtaggtgt ctcagcaatt
     4381 tgtccacccg gtacgctggt gaattataca taccgtagtt atgtcaccaa cttta
//
LOCUS       DBANUU010000242         4407 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000242 DBANUU010000000
VERSION     DBANUU010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.247"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            71..211
                     /locus_tag="ACIE68_004586"
                     /pseudo
     CDS             71..211
                     /locus_tag="ACIE68_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954872.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(405..1382)
                     /gene="wzzB"
                     /locus_tag="ACIE68_004587"
     CDS             complement(405..1382)
                     /gene="wzzB"
                     /locus_tag="ACIE68_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFX5910764.1"
                     /translation="MRVENNNVSGQNLDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLVVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGSAAPKVSEIQSILIGRFSTT
                     FSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGQSPEAAQKQLAQYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSDALESMVKHEASRPLVFSSTYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILVVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(1528..2694)
                     /gene="ugd"
                     /locus_tag="ACIE68_004588"
     CDS             complement(1528..2694)
                     /gene="ugd"
                     /locus_tag="ACIE68_004588"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFX5910765.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDNIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(2942..4348)
                     /gene="gndA"
                     /locus_tag="ACIE68_004589"
     CDS             complement(2942..4348)
                     /gene="gndA"
                     /locus_tag="ACIE68_004589"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFX5910766.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDIGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELIAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDGDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKGEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT         1186 a         1026 c          989 g         1206 t
ORIGIN      
        1 atttttttgc ctggttgcta aataatctgc tgataaaaaa tacagatttg tacaaaatta
       61 cgccatgctc gctgtgagta accccgtttt gcgacggtac atactaacca atgaggtacc
      121 tgatttgatg tatcacctgc tggtgctgtt gcaggttcaa aatctggatt taacaacagt
      181 tatagtgaat ctgcgcacgc ggcatcagtg agttgcgggg taggcggatg tgatgttgct
      241 gtcgcttccg gcaacaactc atacgtcatt gcccggtgtg caggtttatc gtgtctacaa
      301 attttctcaa catatgtaag ctggataaga tacgccggca tcgcatccga caaaaaaacg
      361 ggcacggtgt gaccaccctg cccgctttct tttagctcaa aaaatcactt cgcgttgtaa
      421 ttacgcagcg cgttacgccc cagcacaata cccgcgccga ccatgccgcc cagcagcacc
      481 accagaatca aggtgattgc cttcttaggg ctatcacgac gaatgggtaa cgtaggtttc
      541 atcacatagc ggtaagcgtg aatatcaaga tcatcaacct ttaagctttc gatatccagc
      601 aggttttgac gagtctgata gtaggttgac gagaacacca acggacggct cgcttcgtgc
      661 tttaccatcg actccagtgc atcactcccc aaaaggaaca tggtatcctg ggtcacgtcc
      721 tgtgtctgct gaatctgcgg ctttgtcacc tgcgcctgat tcgcatactg caacgcttcc
      781 tgaatctgac ggatacgcag atctttctgc tcttgcgcga ctacttcctg ggtcttcaga
      841 gaatcctgca agtttttcat acgcagcgcg atgttgtctt tcagatcttt ttcaagttct
      901 tcatttacct ggtcgtcaac ttgctggata tattgagcca gctgtttctg cgcagcttca
      961 ggcgattgcc caacataaga caccgctaaa ggtaatgact gatttttaac agtaggttca
     1021 atagtcagtt tttcaggttc ttcctgatta tcaagcgttt ctgctaatgc agaaaatgtc
     1081 gtgctgaagc gaccaattaa aatcgactgg atctcagaca ctttcggcgc ggctgaacca
     1141 taaatcacat ttatggcatt gttataacca gcgatttgcc ccacatcagg ttgagtaacg
     1201 atagctgtgg aagtccattt ttctttggca acgaccagat atcctattgc cagggcaata
     1261 gcgacaatga cagaaatgat gattgtcatc ttgccacgcc atagttgcac cagtaaatca
     1321 atcaaatcaa tctgttccgg gtcaaggttt tgcccagaaa cattattatt ttctactctc
     1381 atcattaccc taacggacgt tataatttta ggtctgcata gtgtagtgat aatcttagtg
     1441 aatactatag gataaatgat aaaaacaatg aattattgca caggttacaa aagacaatca
     1501 gggcgaaacc gccctgatca gagaagatta gtcgctgcca aagagatctc gtgtgtagac
     1561 tttgtctgcc acatccttaa gctcttctgc catacggttg gagataataa cgtcggcttg
     1621 ttgtttgaag gtcgcgagat cacgttccag gcgagagttg aagaatgagt cttctttcat
     1681 caccggttcg tagatgataa cttcaacgcc tttcgcctta atacgtttca taatcccctg
     1741 aatggaagac gcacggaagt tatctgaacc gctcttcata atcagacgat aaatacccac
     1801 cacttgcggc ttgcgtgaca aaatggcatc ggcaataaaa tctttacgcg tgcggttagc
     1861 gtctacgatt gccgagatca ggttattcgg cacagactgg tagttcgcca gtaactgctt
     1921 ggtatctttc ggcagacaat aaccaccata accaaacgac ggattgttgt aatggttgcc
     1981 aatacgcgga tcgagacaaa cgccttcgat aatctggcga gtattcagac ctaaactttc
     2041 tgcatagcta tccagttcat taaagtatgc tacgcgcatc gccagatagg tattagcgaa
     2101 aagtttaatc gcttctgctt cggttgaatc ggtgaacagg gtcgggatat tttgcttaat
     2161 cgcgccttcc tgtaataacg ccgcaaaacg ttctgcgcgt tccgaacgct caccgatgac
     2221 aatacgtgac ggatgaaggt tatcgtaaag ggctttacct tcgcggagga attcggggga
     2281 gaaaataata ttttcagtac gatatttctt atgcatcgct gcggtaaaac caacgggaac
     2341 cgtggatttg atgaccataa ccgcataagg atttatctct actacgtctt taattactga
     2401 ttctacacta gacgtattga aataattagt tttaggatca tagtcggttg gagtggcgat
     2461 gatgacataa tcagcatcac ggtaggcttc atttttatct aatgtggcat taaagtgtat
     2521 gttatctgat tgcaaaaact gctgaatttc cttatcaaca ataggagata tccgatcatt
     2581 cagcatagca acgcgtgacg gtaaaatatc taatgccaca acctcatgat tttgtgcgat
     2641 tagaagcccg tttgacaagc ctacataacc ggtaccggaa atggtgattt tcatttcatg
     2701 ctctcagaat taacttaact gtgaatcatg atgtttttag catcctgata agagttaaaa
     2761 gttttaacgc tacgtagata gctggtcaac gcaacgaaaa acaattcaga atttctttat
     2821 aagaatagct gatatttatt aagttaatat taagcagtga aatttagtct ataagataat
     2881 tggcaaaaaa aagcccgaca caatatacgc cgggcctcaa tttattgttg gttaaatcag
     2941 attaatccag ccattcagta tggaacacac cttctttatc aatgcgctta taagtatgcg
     3001 caccgaaata gtcacgctgt gcctggatca ggtttgcagg cagaacagcg gcacggtagc
     3061 tgtcataata ggcaaccgca gcagcgaagg tcggaaccgg gataccgttc tgtactgcat
     3121 aagcaacgac atcacgcagc gcctgctggt agtcatcggc aatttgcttg aagtacggag
     3181 ccagcagcag gttagcgatc tgcggatttt cggcataagc atcggtgatt ttctgcagga
     3241 actgcgcacg gataatgcag ccagcacgga aaatcttcgc gatttcgccg tagttcagat
     3301 cccagttgta ctcttcagac gccgcacgca gctgagagaa gccctgagcg taagaaacga
     3361 ttttgcccag atacagagca cgacgaactt tttcgatgaa ctcacctttg tcgccagctg
     3421 gctgcgcttg cgggccagag agaactttag atgcggcaac acgctgctct ttcagagaag
     3481 agatataacg tgcaaacaca gactcggtaa tcagcgacag cggttctccg agatccagcg
     3541 cgctctggct ggtccattta ccggtacctt tgttagccgc ttcatccagg atcacatcaa
     3601 ccaggtagtt accgtctcca tcttttttgg taaagatgtc tttggtgatg tcgatcaggt
     3661 agctactcag ttcaccgtta ttccactcgg taaaggtctg tgccagttct tcgttagaga
     3721 gattcaggcc gcctttcagg agagaatagg cttcagcaat cagttgcata tcaccgtatt
     3781 caataccgtt gtgaaccatc ttcacatagt gacccgcgcc atcggcacca atataggtaa
     3841 cgcacggttc gccatcttca gcaacggcgg cgattttggt caggatcgga gcaatcagtt
     3901 cataggcttc tttctgccca ccaggcatga tggaaggccc tttcagcgcg ccctcttcac
     3961 ctccagaaac accggtaccg atgaagttaa agccttcagc agaaagctca cggttacgac
     4021 gaatggtgtc ctggaagaag gtgttaccac catcaatgat gatgtcacct atatcgaggt
     4081 acggcttgag ggaatcaata gcagcatccg tgcctgcacc tgctttcacc attaacagga
     4141 tgcgacgagg cgtttccaga gattcaacaa actctttcac cgtatagtaa ggaaccagtt
     4201 tcttgcccgg attttcggca atcacttctt ccgtcttttc acgggaacgg ttgaaaatag
     4261 agacggtata accacggctt tcgatgttga gcgcaaggtt gcgccccatc actgccatac
     4321 cgacgacgcc gatctgttgc tttgacattg tttactcctg tcaggggggc ttacttagtt
     4381 taatagatct gatagattat gtgagca
//
LOCUS       DBANUU010000243         4403 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000243 DBANUU010000000
VERSION     DBANUU010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.248"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            483..707
                     /gene="hha"
                     /locus_tag="ACIE68_004590"
     CDS             483..707
                     /gene="hha"
                     /locus_tag="ACIE68_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFX5910767.1"
                     /translation="MQHKVTRDEWKLKPEQFRRCSSRETLEKVSSHTRYKLTPAELEA
                     FNSAVDHRLAELTMNKLYDRVPASVWKYVT"
     gene            complement(1040..1237)
                     /locus_tag="ACIE68_004591"
     CDS             complement(1040..1237)
                     /locus_tag="ACIE68_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910768.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGAVDPFGYYRKTDRLLCLL
                     TLLLVLILVTVSGVSVFVLLQ"
     gene            1436..2005
                     /locus_tag="ACIE68_004592"
     CDS             1436..2005
                     /locus_tag="ACIE68_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFX5910769.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGRYDTLAHFPDKPAVWLERDDETGFQ
                     QVLERAGYLAKEHTKARGTGERNFGCSRG"
     gene            2171..2554
                     /locus_tag="ACIE68_004593"
     CDS             2171..2554
                     /locus_tag="ACIE68_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFX5910770.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGRFSEEGVAE"
     gene            2551..2976
                     /locus_tag="ACIE68_004594"
     CDS             2551..2976
                     /locus_tag="ACIE68_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFX5910771.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCGRLF
                     PGKKFCEACGTGIYCRAVCTKSVYNYQPDLFLDCGSAST"
BASE COUNT         1076 a         1107 c         1158 g         1062 t
ORIGIN      
        1 gagttcatat atgtgaaata aatctgtgag tgtgatcagc agaccagtct acccagtagc
       61 aacagattgc gttgtactca cagggttatc cctgataact ggcgtataac ctgcgtacat
      121 aaaacgtacc tgcaatgcac ctgaacagag tgcattaagc atccgcctga gcggtaaacg
      181 tatccgatct ggtgagtgac agtctgagag cattctcagt aatcgccccc ttgtatcctg
      241 gaaattggtt aagagcgatt agatttggca aggtgtatgc agccagcagc ttaacacctc
      301 agctaactgg ctggttacct tcaggttgtc gcaatggcat cggatatccg gtgtcggata
      361 actaaacgta gcgttaaggc gatattgctg agagaccggt taagtggtgg cacgggagta
      421 atgagtttgg ggagggtacc agaatgccag tcaggtgcgg tatgctggca gtatcacgtt
      481 gattgcagca taaagtaacg agagacgaat ggaagttaaa accagagcag ttccgtcgtt
      541 gttcatcccg ggagacgctg gaaaaagtga gttcccacac acgttataaa cttacccctg
      601 cggagctgga agcctttaac tctgcggtcg atcaccggct ggcagaactg acaatgaaca
      661 aactttacga tcgtgtgccg gcttccgtct ggaaatatgt cacctgaatc ttgaatccgc
      721 gctacgatga ctccggtgtt atctgtgcac cggagaaccg ggaacattaa gcggtatagc
      781 gacaacaaag aattcgaaat caaaagggaa tggtgtacag aggggcaggc gttcaggcat
      841 taccggcctg tatcttctga aggggactcg tatttgtcgg ggctgagtat tccgctcgat
      901 tcgatcacgg attggcagaa tacctgactg tgattgattc gggtaccatt ttgcagggat
      961 ccggtgcgtt actgtctggc acaacaaacg cagcgggtcg ctctgccccg ccgattcctg
     1021 ataatccctc cccagtaacc tactgcaaca gtacaaatac actgacgcct gaaacggtaa
     1081 ccagaatcag caccagcagt aacgtcagta aacagagcag acgatcggtc ttcctgtaat
     1141 aaccgaaggg gtcgacagcg ccacaatacg ggcaacatac tgtattatga gtaattgcgt
     1201 taccacagtc cctgcacttc tttttgcgta tcacgatcgc tccctctcca cgccttatcc
     1261 gtaaccggtt ttttacatta aaaaaacaac gccgggaatt caatcgtcag tccggcgacg
     1321 accgttcggg ttatcacaga aagcctgaga cagtgtcctg ccggaggtca cttcaggggt
     1381 tctgcgcatt ttttacgacg tggctattcc gtatgaacca tacggagatt taaccatgac
     1441 ctacaaatac aaccccttct ggcagcaacg tattcgtgag acggtgcggc acgcactgga
     1501 tgttcatccc cgcctgacgg cacttcgggt tgatctgcgt ctcccggatg tgccggcagc
     1561 aacggacgcg gctgtgatat cccgcttcat caatgccctg aaagcccgaa tcgacgctta
     1621 ccagaaacgt aagcatcggg aaggtaaacg cgtgcattcc tcgaccctgc attacgcctg
     1681 ggcccgggag ttcggggagc ttaaaggcaa aaaacactat cacctgctgc tgctggtcaa
     1741 ccgggatacc tggtgtcgtg ccggcgatta ccgcgctccg gggtcactgg ccgggatgat
     1801 taaacaggcc tggtgcagtg ccctgggagt ggatgccgga cgttatgaca cgctggcaca
     1861 ttttcctgac aagccagcgg tgtggctgga acgcgatgat gaaaccggct ttcagcaggt
     1921 gctggaacgt gctggctatc tggcgaagga gcataccaaa gctcgcggca ccggtgagcg
     1981 caactttggc tgcagtcgtg gctgagccag acagacgagt tatctgactt cagtgtgatg
     2041 acgctctcac ggccaccaca ctgattttcc cttctgagac tgaactgaaa ccgccattca
     2101 cacctgatca cgctgcccgt accgggacgg ctgttgcctg cgtgtcgtct gacggtaagg
     2161 aacatatact atgtacgcaa aatcctttct cgctcttgat ggcaacggac gtctgacggg
     2221 ggcccgtacc gcacagactg cgccttacga ccgctacacc tgccaccttt gcggcagtgc
     2281 actcagatac cacccgcaat acgacactga acggccctgg tttgaacacg ctgacgaagg
     2341 gctgacggag cacggtcatg agtgtcctta tgtcaggccg gaacgcagag aaatacggtt
     2401 gattaaacgt ctgcagcaat tcgtcccgga tgccttaccc gtggtgcgta aagccagctg
     2461 gcactgcaga caatgtcacc acgattatta tggggagcgg tactgcacac actgccagac
     2521 cgggcgcttc agtgaggagg gggtagcaga atgaacggta taaaaacggg gctggggatc
     2581 acgccgggtg aacacattat cagtgccgac tctgcgctca gcagaaatat ccggcagtgt
     2641 ttctgccttt cctgccatgg ccggttgatt ctgcagacag atgctcaggg ggcgtggttt
     2701 gagcatgatt tacatgccct gagtgagcaa cagaaggcgg actgtgtggt gctgaatccg
     2761 gaaaagtccc acccttacat tgaggatatg gcgatgtttt tatcgccatt accggtcgtg
     2821 ctggagtggc attgtgtgat gtgcgggagg ctttttcccg ggaagaagtt ctgtgaggcc
     2881 tgtggtacgg ggatttattg cagagcggta tgtacgaagt ctgtttacaa ctaccagcca
     2941 gacttgttcc tggactgcgg aagtgcgagt acttaatccg aaatccccgc tctgcgcagg
     3001 gcgttcagag gtattgccaa cctgtatttt atccggtgtt tcgcacccac cccgttcgtg
     3061 acacaaaggc gcgacaccgg atatccgcag tcctgcttac ctgaataaaa cccggggtat
     3121 tgattggtag tgtggtgcgg aatcgtaccg tgatctcgca ttcgccgaat tcgcactgtg
     3181 gtgtcaatca accacacggg ggcgtcgtct cagcctttgc ctgcctcagg agggtgaaac
     3241 ctggggggca ttgattaata tcctgcccga tctgatgacc ggtacttcag acctgctacc
     3301 gatacgccgg aatataacgg tcagagaggc atatttcgat gcccctgttc tggagcagtt
     3361 gatgatggat gccataggca atgtaagcgc tttgtatcac cggacgaagc acgcagtacc
     3421 agaagccccc cggggttcat tggtgacctg acagcgaaga tagattcaaa gagcgttcag
     3481 tgatttttcc tgcgctcgtc gtggtgtacg caatcaggaa tcccgttatc tcattggcat
     3541 acttgcgata ccacaccagc aggacgacgc tgaaatcacg ccgacattct cctgtatggc
     3601 atcaatctca caggggacat tctgagcatg ttgacctcaa tcacagatca cgacagcgtg
     3661 ctcattttac tgtcttatat cattggtgct gacgcaccgg cccgacaaag ggttttacac
     3721 gctgattcag tgaagaccat ttctctaaac ccatcaaact ggggtgaagc aaccgcaagt
     3781 acaaaagtga agtgccacag tagatacagc aacgaatcat ggaggtcaga agtatgaaac
     3841 gtgttgtgat accggtacgc tggcaatgtg caaaatgcca gcgctggtat tgtggaaccc
     3901 ggccctgccc ttggtgctgg cgatattccc gttcatcttc ctgctgatac cttccgttca
     3961 gcagcgggtt gttatccctt cctgactgat tcgtcattcc attcttattg attagaactg
     4021 gcattacacc ggtgccggcg tgctttcctg cgtgtctgcg ccggttcagt aaattagaaa
     4081 agaaacattt gtcagcgcca gtgatataag gcgtaacggc taacaatctt ggcgttaata
     4141 cattccgagc aaataaccga agcgttactt ttagtgacgg ggttatccaa tacttttcta
     4201 gtcagtgttt agtatttatg caagcgatat aagtagtcac cgcttagact attgacattc
     4261 atgatgcctg ttaattcaaa tttcaggtat gatcagtcat ttattgttgt gatactgatt
     4321 ccgctagttt agcataactg tcctgcaaat cattgttcaa aaaagaaagc tcctacaaac
     4381 tgtatcctgt atctctacca gaa
//
LOCUS       DBANUU010000244         4382 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000244 DBANUU010000000
VERSION     DBANUU010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.249"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>4382
                     /gene="traI"
                     /locus_tag="ACIE68_004595"
     CDS             <1..>4382
                     /gene="traI"
                     /locus_tag="ACIE68_004595"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFX5910772.1"
                     /translation="IREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKET
                     GFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQFTYTDVLAR
                     TVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIM
                     KQNWVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMARE
                     QGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGNTVIVDQGEKLSLK
                     ETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEP
                     DRNVRYARLAGDFAASVKAGEESVAQVSGVREQVILTQAIRSELKTQGVLGHPEVTMT
                     ALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGET
                     QVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVV
                     VPGRAEPASLPVADSPFTALKLESGWVETPGHSVSDSAKVFASVTQMAMDNATLNGLA
                     RSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPA
                     QQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTSFTDLGGEINAQIKRGDLLYVDV
                     AKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
                     LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESGRPRVVGLGPTHRAVGEMRSAGV
                     DAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVA
                     SGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINRDVERALSG
                     LESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAI
                     VRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVPVLNTANIR
                     DGELRRLSTWETHRDALVLADNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEG
                     VTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSGGQQTRVIRPA
                     QVRAEQHIDLAYAITAHGAQGASETFAIALEGTEGSRKLMAGFESAYVALSRMKQHVQ
                     VYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLR
                     QAGLAGGDTPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGE
                     GRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGA
                     ITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENK
                     PDLPDSKTEQAVREIAGQERDRSA"
BASE COUNT          954 a         1157 c         1495 g          776 t
ORIGIN      
        1 atccgggagg ccgtcgggga agatgcctcg ctgaaatccc gggatgtggc ggcactggat
       61 acgcgaaaat ccaaacagca cgtcgatcct gaagtcagaa tggctgaatg gatgcagacg
      121 ctgaaggaaa ccgggttcga catccgggca tatcgtgacg cggcggatca gcgtgcggag
      181 acccgcacgc aggcgcccgg ggctgtttca caggaggggc cggatgtgca gcaggcggtg
      241 acacaggcca ttgccggatt aagtgaacga aaagtgcagt ttacgtacac ggacgtgctg
      301 gccagaacgg tcggcatact gccgccggaa aatggtgtga ttgaacgggc acgcgccggt
      361 atcgatgagg ccatcagccg tgagcagctt atccccctcg atcgtgagaa ggggctgttc
      421 acgtccggga ttcatgtgct cgatgagctg tcagtccggg cactcagtcg tgacatcatg
      481 aaacagaact gggtgaccgt acatccggag aaaagtgtcc cccggacggc cggttacagc
      541 gatgccgtca gtgttctggc acaggatcgc ccgtcgctgg ccattgtgtc cgggcagggc
      601 ggtgcagccg ggcagcgtga gcgggtggct gaactggtca tgatggcccg ggagcagggg
      661 cgggaggtgc agattatcgc tgctgaccgt cgctcgcaga tgaacctgaa gcaggatgaa
      721 cggctgtccg gtgagctgat aaccggacgt cgtcagctgc tggaaggcat ggccttcacg
      781 ccgggcaata ctgttatcgt tgaccagggc gaaaaactct ccctgaaaga gacgttaacc
      841 ctgctggatg gtgccgcacg tcataacgta caggtcctga taaccgacag cgggcagcga
      901 accggtacag gcagtgcact tatggccatg aaggatgccg gggtgaacac atatcgctgg
      961 caggggggag aacagcgacc ggccaccatc atcagtgaac cggaccgcaa tgtccgttat
     1021 gcccggctgg cgggagattt tgcggccagc gtgaaagccg gagaagagag cgtggcacag
     1081 gtcagcgggg tacgggaaca ggtcatactg acacaggcca ttcgcagtga gctgaaaaca
     1141 cagggcgtgc tcggacaccc ggaggtgacc atgaccgccc tgtcaccggt ctggctggac
     1201 agccggagcc gttatctgcg ggatatgtac cgcccgggga tggtgatgga gcagtggaac
     1261 ccggagacac gcagtcatga ccgctatgtg atagaccggg tgacggcgca gagtcacagc
     1321 ctgaccctgc gggatgcgca gggtgaaacg caggtggtgc gtatttcttc tctggacagc
     1381 agctggtcgc tgttccggcc ggaaaaaatg ccggtggcag acggcgagcg tctgagggtg
     1441 accgggaaaa ttcccggact ccgcgtctcc ggcggtgacc gcttgcaggt ggcatccgtc
     1501 agcgaggacg cgatgacggt tgttgtgccg ggacgggctg aaccggcctc cctgcctgtg
     1561 gctgattcac cgttcacggc actgaagctg gagagcggct gggtggaaac acccgggcat
     1621 tccgtcagtg acagtgcgaa ggtttttgcc tccgtcacac agatggcaat ggacaatgcc
     1681 accctgaacg gtctggcccg cagtggtcgt gatgtccggc tgtattcctc actggatgaa
     1741 acccgtactg cggaaaaact tgcccgccat ccctccttta cggtggtttc tgagcagata
     1801 aaggcgcgtg ccggtgagac attgctggaa accgctatca gtctgcagaa agcagggctt
     1861 cacacgccgg cacagcaggc cattcatctg gcccttcctg tgctggaaag taaaaacctg
     1921 gccttcagca tggtggacct gctgacagag gcgaagtcgt ttgctgcaga aggaaccagt
     1981 tttactgacc tgggagggga aatcaatgcg cagataaaac gcggtgattt actgtatgtg
     2041 gatgtggcaa aaggctatgg cacaggcctg ctggtttccc gtgcgtcgta tgaggctgaa
     2101 aaaagcattc ttcgccatat tctcgaaggt aaggaggcgg tcacgccgct gatggagaga
     2161 gtacctggcg aactcatgga gaaactgaca tcaggacagc gcgccgccac ccgcatgata
     2221 ctggaaacgt ccgaccgttt cacggtggtg cagggctatg ccggtgtggg gaagaccaca
     2281 cagttccggg cggtgatgtc agccgtgaac atgctgccgg agagtgggcg tccccgtgtc
     2341 gtggggctgg gtcccacgca ccgcgcggtc ggggagatgc gcagcgccgg cgtggatgca
     2401 cagacactgg cgtcatttct gcatgacacg cagctgcagc agcgcagcgg agaaacaccg
     2461 gatttcagca acacgctgtt cctgctcgat gagagctcaa tggtgggcaa taccgacatg
     2521 gcacgggcat acgccctgat tgcggccggt ggtggtcgtg ccgtggccag cggtgacacg
     2581 gaccagctgc aggccatcgc gcccggtcag cctttccgtc tccagcagac gcgcagtgct
     2641 gccgatgtgg ctatcatgaa agagattgtg cgtcagacgc cggaactgcg ggaggcggta
     2701 tacagcctga ttaaccggga tgtggaaagg gcattgtccg ggcttgagag tgtgaaaccg
     2761 tctcaggtgc ctcgtcagga gggcgcatgg gcaccggagc actctgtgac ggagttcagt
     2821 cacagccagg aagcgaaact ggcagaagcg cagcagaagg cgatgctgaa aggcgaggct
     2881 ttcccggatg tccccatgac actgtatgaa gccattgtcc gcgactatac cggcagaaca
     2941 ccggaagcac gggagcagac gctgattgtc acgcacctga atgaggaccg gcgcgtactg
     3001 aacagcatga ttcatgatgc acgggaaaag gccggtgagc tggggaaaga gcaggtcatg
     3061 gtgcctgtcc tgaacacagc gaatatacgc gacggagagc tgcgtcgtct ctccacctgg
     3121 gagacacatc gggacgcact tgtcctggcg gataatgtgt atcaccggat tgccggtatc
     3181 agtaaggatg acgggctgat aaccctgcag gatgcggaag gtaatacgcg gctgatttcg
     3241 ccccgggagg cggtggctga aggcgtcaca ctgtacaccc cggacaccat ccgggtaggg
     3301 accggtgacc ggatgcgctt cacgaagagt gaccgggagc gtggttatgt ggccaacagc
     3361 gtctggacgg tgacagcggt ttccggtgac agtgtcacgt tgtcgggcgg acagcagacc
     3421 cgggtgattc gccccgccca ggtgcgggca gagcaacata ttgacctggc ctatgccatc
     3481 accgctcacg gtgcgcaggg ggcaagtgaa acctttgcca tcgcgcttga aggtacggaa
     3541 ggcagccgga aactgatggc tggctttgag tcagcctacg tggccctgtc gcgtatgaag
     3601 cagcatgtgc aggtgtacac cgataaccgt cagggctgga cggatgccat taacaatgcc
     3661 gtacagaaag gaacagccca cgatgtgttt gaaccgaaac cggaccggga ggtcatgaat
     3721 gcagagcggc tgttcagtac ggcgcgggag ctgcgggacg tggcggcagg gcgtgccgtt
     3781 cttcgtcagg cggggcttgc cgggggagac actcctgcac ggtttattgc tccgggacgt
     3841 aaatatccac agccgtatgt ggcactgccg gcgtttgacc gtaacggcaa gtctgccggt
     3901 atctggctga acccgctgac cacggatgac ggaaacgggc tgcggggatt cagtggtgaa
     3961 ggccgtgtga aaggcagcgg ggatgcgcag ttcgtggccc tgcagggcag ccgtaacggc
     4021 gagagcctgc tggctgataa tatgcaggat ggtgtccgga ttgcccgtga taaccccgac
     4081 agcggtgtgg tggtgagaat cgccggtgaa ggtcgtccgt ggaatcccgg tgccatcacc
     4141 ggtggccgcg tgtgggggga tatcccggac aacagcgtcc agccgggagc cggaaatggc
     4201 gagccggtca cggcagaggt actggcacag cggcaggctg aagaggccat ccgccgtgaa
     4261 acggaacgcc gcgcagatga aattgtccgt aaaatggcag agaacaaacc tgacctgccg
     4321 gacagcaaaa cagagcaggc tgtcagggag attgccgggc aggagcgtga ccggtctgcc
     4381 at
//
LOCUS       DBANUU010000245         4349 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000245 DBANUU010000000
VERSION     DBANUU010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.250"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..218)
                     /locus_tag="ACIE68_004596"
     CDS             complement(<1..218)
                     /locus_tag="ACIE68_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910773.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPF"
     gene            447..1055
                     /locus_tag="ACIE68_004597"
     CDS             447..1055
                     /locus_tag="ACIE68_004597"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFX5910774.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1153..2544
                     /gene="selA"
                     /locus_tag="ACIE68_004598"
     CDS             1153..2544
                     /gene="selA"
                     /locus_tag="ACIE68_004598"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFX5910775.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2541..>4349
                     /gene="selB"
                     /locus_tag="ACIE68_004599"
     CDS             2541..>4349
                     /gene="selB"
                     /locus_tag="ACIE68_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFX5910776.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVVLTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDH"
BASE COUNT          972 a         1118 c         1286 g          973 t
ORIGIN      
        1 atgaacggca gcgggccggg cagggcgatg tcggtttcac cgggaaggac ctttgcaccg
       61 agcaggggat tgaccggatg gccggacgtc actccgccgg ggcacaccga acaggccacg
      121 ccggtggggg caccaatgcg caccccggct gaaccctgaa caatgctacc gccatactgc
      181 gtcatgtcgc cctgacgcgc tgccggtttt ccgctcatct gctcatcctt gttttatcgg
      241 gttatttgtc tgcttccagg caaaacccta cagatttata agaaagcctt aagtgagata
      301 tccatcagat agaaacaatt aggaaaatat ttatatcacc atgataaata tgtgatttat
      361 ttggaagtaa caaaccatca acattttctg ccctttttcc catcaccagg ccatactgat
      421 atttttcctc gcacaaagga ctcggcatga aactcgtcgg tagctacacc agcccgtttg
      481 tacgcaaact ttctattctg ttgttagaaa agggcataac tttcgaattt attaatgaac
      541 tgccctataa cgcggacaac ggcgtggcgc aatttaaccc gttaggaaaa gtgccggtgc
      601 tggtgaccga agagggcgaa tgttggtttg attcgccgat catcgctgaa tatattgaat
      661 taatgaatgt cgctccggcg atgttgccgc gcgatccgct ggagtcgttg cgggtgcgca
      721 aaattgaggc actggcggat ggcattatgg atgccgggct ggtatcggtg cgtgaacagg
      781 cgcgtccagc ggcgcagcag tctgaagatg aattgttacg ccagcgggag aaaatcaacc
      841 gcagtctgga tgtgctggaa ggatatctgg tcgatggcac actcaaaacc gatacggtca
      901 atctggcgac tatcgccatt gcctgtgccg tcggatatct caatttccgc cgcgttgcgc
      961 caggttggtg tgtcgaccgc ccacacttag tcaaactggt cgaaaacctg tttagccgcg
     1021 aaagttttgc tcgcaccgaa ccgccaaagg cttgatgcgc gatatgtcct cctgacccat
     1081 ctcacgttac aatccgtggt tatgttaaac gcccttctcc gtgtgagagg gccttgatca
     1141 gccaggtttc ctatgacaac cgaaacgcgt tccctctata gtcaacttcc ggctattgat
     1201 cgcttattgc gcgatagctc cttcctttct ttgcgtgata cttatggtca cacccgcgtg
     1261 gtggaattgt tgcgtcagat gctcgacgaa gcgcgagaag tgattcgtgg cagccagacg
     1321 ctgcctgcgt ggtgtgaaaa ctgggcgcaa gaagtcgatg cccggttgac gaaagaagcg
     1381 cagagcgcgc tgcgtccggt gatcaacctg acgggaaccg tgctgcatac caaccttggg
     1441 cgagctttac aggcggaagc cgcggtggaa gccgttgcgc aggctatgcg ttcgccagtg
     1501 accctcgagt acgatctgga cgacgccgga cgcggacatc gcgatcgggc gctggcgcaa
     1561 ctgctgtgcc gtattacggg ggcggaagat gcctgtatcg tcaataacaa tgcggcggcg
     1621 gtgttattga tgttggcggc cactgccagc ggaaaagagg tggtggtatc tcgcggcgaa
     1681 ctggtggaga ttggcggcgc gtttcgtatt cccgatgtta tgcgtcaggc aggctgcacc
     1741 ctacacgaag tagggaccac caaccgcacg cacgcgaatg attatcgtca ggcggtgaat
     1801 gaaaataccg cactgttgat gaaagtacat accagtaact acagcattca ggggttcacc
     1861 aaagcgatag atgaagcgga actggtggcg ctcggcaaag agctggatgt tcccgtagtg
     1921 actgatttag gcagtggctc gctggtcgat cttagccagt acggtttgcc gaaagagcca
     1981 atgccgcagg agttgattgc ggcgggcgtc agtctggtga gtttctccgg cgacaagttg
     2041 ttaggcgggc cgcaggcagg aattattgtt ggtaaaaaag agatgatcgc ccgcctgcaa
     2101 agccacccgc tgaagcgtgc attacgcgcg gataaaatga ccctcgcggc gctggaagcc
     2161 acgttgcgtc tttatttaca ccctgaagct ctgagtgaaa aattaccgac cctgcgcctg
     2221 cttacccgca gcgcagaggt cattcaaatc caggcacaac gtttacaggc cccccttgcc
     2281 gcacattacg gcgcggagtt tgcggtacag gttatgccat gtctttcgca gattggcagt
     2341 ggttcgctgc cggttgatcg cctgccgagc gcggcattaa cgtttacacc ccatgatgga
     2401 cgcggtagcc accttgagtc attagccgcc cgctggcgtg aattgccagt gccggtgatt
     2461 ggtcgtattt atgacggacg attgtggctg gatttacgct gccttgaaga tgagcaacgg
     2521 tttttggaga tgttgttgaa atgattattg cgactgccgg acacgttgac cacggcaaaa
     2581 caaccttatt gcaggcgatt actggcgtaa atgctgaccg tctgccggaa gaaaaaaagc
     2641 gcggcatgac catcgatctc ggctatgcct actggccgca gccggatggt cgcgtgcctg
     2701 gttttatcga cgttcccggt catgaaaaat ttctttccaa catgctggcg ggcgttggtg
     2761 gtatcgatca cgcgctgttg gtggtggcgt gcgatgacgg tgtgatggca cagacccgtg
     2821 agcatctggc gattttgcag ctgaccggta acccgatgct gacagtggtg ctgaccaaag
     2881 ccgatcgcgt ggacgaagcg cgtgttgatg aggttgaacg tcaggtaaag gaggtgctgc
     2941 gggaatacgg ttttgctgag gcaaaactgt ttatcaccgc agcaaccgaa ggtcgaggaa
     3001 ttgatgccct gcgcgagcat ctgcttcagt taccagaacg tgaacacgcc agccaacata
     3061 gtttccgcct cgcgatcgac cgcgcattta ccgtaaaagg tgccgggctg gtcgtcaccg
     3121 gtacggcgtt aagcggggaa gtgaaggtag gcgattcact atggctaact ggtgtaaata
     3181 aaccgatgcg tgtacgtgcg ctgcatgcac aaaaccagcc aacagaaacc gcccatgccg
     3241 ggcagcgtat cgcgcttaac atcgcgggtg atgcggaaaa agagcagatt aaccgtggcg
     3301 actggctgct tgccgatgcg ccgccagagc cgttcacacg ggtgattgtc gagcttcaaa
     3361 cccatacgcc gctgacccag tggcagccgc tgcatattca ccacgccgcc agccacgtta
     3421 cgggacgcgt ttcgctgctg gaagataacc ttgccgagct ggtgttcgac acgccgctgt
     3481 ggctggcgga taacgatcgc ttagtgctgc gcgatatctc ggcccgcaac acgctggcgg
     3541 gggcgcgtgt ggtgatgctt aacccgccgc gtcgcggtaa acgtaagccg gaatatctgc
     3601 aatggctggc gtctcttgca cgggcgcaga gtgatgccga tgcgttatct gttcatctgg
     3661 aacgcggcgc ggttaacctt gcggatttcg cctgggcgcg ccagctcaac ggcgaaggga
     3721 tgcgcgaatt gctgcaacag cctggctata ttcaggctgg ttatagcttg ttgaatgcgc
     3781 cggttgccgc ccgctggcag cggaaaattc tcgacacatt agcgacttat catgagcaac
     3841 atcgcgatga acctgggcct gggcgcgaac gtctgcgacg tatggcgttg ccaatggaag
     3901 atgaagcgct agtactgttg ctgattgaaa agatgcgcga aagcggcgac atcctcagtc
     3961 atcacggttg gctgcatctg ccggatcaca aagcgggctt cagcgaagag cagcaggcca
     4021 tctggcaaaa agcagagccg ctgtttggtg acgagccgtg gtgggtgcgt gatctggcaa
     4081 aagagacggg taccgacgag caggcaatgc gcctgacgct acgccaggcg gcgcagcaag
     4141 gaataattac cgcgatcgtc aaagatcgtt attaccgtaa cgatcggatt gtcgaatttg
     4201 ccaatatgat ccgcgatctc gatcaggagt gtggttcaac ctgcgcggcg gatttccgcg
     4261 atcgcttagg cgtaggccga aagctggcaa ttcagattct ggaatatttt gaccgcattg
     4321 gctttacgcg tcgtcgtgga aatgatcat
//
LOCUS       DBANUU010000246         4346 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000246 DBANUU010000000
VERSION     DBANUU010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.251"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..196
                     /locus_tag="ACIE68_004600"
     CDS             <1..196
                     /locus_tag="ACIE68_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFX5910777.1"
                     /translation="LIEATLKGFKSGELSFYEYRKNLATAGVFRWITNIHENKRYYYT
                     FDNSLLFTENIQNTTQIFPH"
     gene            complement(483..740)
                     /locus_tag="ACIE68_004601"
     CDS             complement(483..740)
                     /locus_tag="ACIE68_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910778.1"
                     /translation="MSDRVIECASRAGRDFSEFMKGEKGMMEALSSVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(737..1234)
                     /locus_tag="ACIE68_004602"
     CDS             complement(737..1234)
                     /locus_tag="ACIE68_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFX5910779.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1436..1894)
                     /locus_tag="ACIE68_004603"
     CDS             complement(1436..1894)
                     /locus_tag="ACIE68_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFX5910780.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1891..2388)
                     /gene="rrrD"
                     /locus_tag="ACIE68_004604"
     CDS             complement(1891..2388)
                     /gene="rrrD"
                     /locus_tag="ACIE68_004604"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFX5910781.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETMRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2388..2603)
                     /gene="essD"
                     /locus_tag="ACIE68_004605"
     CDS             complement(2388..2603)
                     /gene="essD"
                     /locus_tag="ACIE68_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFX5910782.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            3175..4257
                     /locus_tag="ACIE68_004606"
     CDS             3175..4257
                     /locus_tag="ACIE68_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFX5910783.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
BASE COUNT         1188 a         1031 c          936 g         1191 t
ORIGIN      
        1 ccttatagaa gccaccttaa aaggattcaa atcaggagag ttatcatttt acgaatacag
       61 gaaaaatctt gctactgcag gggttttcag atggattaca aatatccacg aaaacaaaag
      121 gtattactat acctttgata attcattact ctttactgag aacattcaga acactacaca
      181 aatatttccg cactaaatca taacgtccgg tttcttccgt gccagaaccg gactcgctgg
      241 catgatgaaa tatgtgtacc cggtaacccc ggtgtgcatc gtttttgatt attcccacac
      301 actcgcgcag aaggagttcc ccgtcgggct acggtctctg ttaatacggg aatacggcga
      361 cgatacagcg catgatgtgt caggcttgaa tacctttatc ctttaaaagg gatatcagtt
      421 aagttatccc gtgtagggta taagccatta tcaagcccac ccgtagatag gctttgtaat
      481 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      541 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      601 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgagga caatgcttcc
      661 atcatgccct tctcaccttt catgaactct gagaagtcac gccccgctct ggaggcgcat
      721 tcaatgacac gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      781 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgccgcc agcccttgtc
      841 cgcagaagct cgccatatct tttttctgat caaactcatg acacttcata ttgagctgtg
      901 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatccagg actttatagg
      961 ctgcatattt aaatccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
     1021 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1081 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1141 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1201 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1261 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1321 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1381 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1441 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1501 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1561 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1621 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1681 gctcctttgt gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1741 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tccttgtagg
     1801 cgattgcgtt atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa
     1861 ccagagcgga gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg
     1921 ctcaatctca cgacgagtca tgagaccttt ccattgctta ccgccagcat atgtccagcg
     1981 acgtagctga tcacatgcgc ctttgatatc gccctggttt attttgcgaa gaagcgtcga
     2041 tgttctgaaa ttgccagcac ccacgttgta aacgaatgag taaagagcgc cgcgcattgt
     2101 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgaccgtgg caaggtcttt
     2161 attcaggagg gctttgcatt ctgctttggt atacgtttta ccgagcatga tgtcttttcc
     2221 tgtatgcccg tgacatacag tccatacacc aacaatatct ttgtatggta tgtagctgac
     2281 accttccaga ccatcgttac cacttgggcc agtgattaac actgatgcta tagcaattgc
     2341 tccgccacca atagcagcag caacggcttt tcgtaatgat ggaggcatta ttcacctctc
     2401 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     2461 ccaaatacca ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct
     2521 agcagctgta aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt
     2581 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     2641 gaatactacg aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2701 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2761 gaagtaacca ctcttaacag cataaccaat tttttacgta cgtaaaccac taaatgatat
     2821 ttgcgagaat gctaccgagt attgaaaaca ccactacaaa tacataagca aatctcaaca
     2881 aataaccaac aaataatttc cagcgttatt tttagccgat ttaaattgaa ccttcaaatt
     2941 atagagcact tataaataac agccattaat ataaattggc taatagattt atttttattc
     3001 agccaagagc catgaatagg attcgataga aaaagttcag ataaaaatag agatctactt
     3061 cacaaatcaa atgagaaacc aaaacttaca tcttgaaata atcacattga ttagatgaat
     3121 atttatcgcg cagtgacatc attttttaat aatagttcaa aaaaagggct cacgatgaaa
     3181 aaattaacag tggcaatttc tgctgtagct gcatcagtac tgatggcgat gtctgctcag
     3241 gcagctgaaa tttataataa agacagtaac aagctggatc tgtacgggaa agttaatgct
     3301 aagcactact tctcctctaa tgatgcagat gatggtgata ctacttatgc ccgtcttggc
     3361 ttcaaaggtg aaacccaaat caacgatcaa ctgactggtt tcggtcagtg ggaatatgaa
     3421 ttcaaaggca accgcgctga atctcaaggt tcctccaaag acaaaacccg tcttgcattt
     3481 gcaggcctga aattcggtga ctacggctca atcgattacg gccgtaacta cggtgtagca
     3541 tacgacatcg gtgcgtggac tgacgttctg ccagaattcg gtggcgatac ctggacccaa
     3601 acagatgtgt tcatgactgg tcgcaccact ggtgttgcaa cttatcgtaa caacgacttc
     3661 tttggtctgg ttgatggtct gaactttgct gctcagtatc agggtaaaaa tgaccgcact
     3721 gacgtaactg aagctaatgg tgatggtttc ggtttctcca ctacttatga gtatgaagga
     3781 ttcggtgtag gtgcaaccta tgctaaatct gaccgcacta ataatcaggt tatctacggt
     3841 aacaacagcc tgaatgcatc tggtcaaaat gctgaagtat gggcagctgg tctgaaatat
     3901 gatgcgaaca acatctatct ggctaccacc tattctgaaa cccagaacat gactgttttt
     3961 ggtaataacc atattgccaa caaagcacaa aacttcgaag tagttgcaca atatcagttc
     4021 gacttcggtc tgcgtccgtc cgttgcttac ctgcaatcta aaggaaaaga cttgggtgcg
     4081 tggggtgatc aggacctggt tgaatatatt gatgtaggtg caacctatta cttcaacaaa
     4141 aatatgtcca cttttgttga ttacaaaatc aacctgattg ataagagcga tttcacgaaa
     4201 gcatctggcg ttgctaccga tgatatcgtt gctgtaggta tggtttacca gttctaattt
     4261 gattactaaa agatatgttg cgggaggctt tgcctcccca acatataagt ggctccctca
     4321 agccacttcc tttaggagca caacct
//
LOCUS       DBANUU010000247         4326 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000247 DBANUU010000000
VERSION     DBANUU010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.252"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            127..225
                     /locus_tag="ACIE68_004607"
                     /pseudo
     CDS             127..225
                     /locus_tag="ACIE68_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
     gene            complement(245..835)
                     /locus_tag="ACIE68_004608"
     CDS             complement(245..835)
                     /locus_tag="ACIE68_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910784.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDLERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVQLNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQINESAAP"
     gene            complement(835..1092)
                     /locus_tag="ACIE68_004609"
     CDS             complement(835..1092)
                     /locus_tag="ACIE68_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910785.1"
                     /translation="MDEERVFSLSYEQLTRFAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            1660..2073
                     /locus_tag="ACIE68_004610"
     CDS             1660..2073
                     /locus_tag="ACIE68_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910786.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGALHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GVVVTVRTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            2075..2854
                     /locus_tag="ACIE68_004611"
     CDS             2075..2854
                     /locus_tag="ACIE68_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFX5910787.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDTWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFLMPESDAVSMLKRIP"
     gene            3034..3678
                     /locus_tag="ACIE68_004612"
     CDS             3034..3678
                     /locus_tag="ACIE68_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFX5910788.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            3765..4073
                     /locus_tag="ACIE68_004613"
     CDS             3765..4073
                     /locus_tag="ACIE68_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HFX5910789.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
BASE COUNT         1013 a         1072 c         1117 g         1124 t
ORIGIN      
        1 gctgataatt taacatattt ttgagcactt ttctttcacc caatgggtga aagctgaatt
       61 tcgaaggaat gcatatttat caaggctttg attatgcttt ttgaagtact gacgtagaat
      121 ctaggtataa gtatcacggg gggttttttg tccgggctgt gcatggcctc tttccactgg
      181 tggcgcagga aagttaaccg gctgccacca tgggatgatg tgtaaaccgt gcacaggcgt
      241 gttcttacgg agcggcactt tcatttatct gtacgcgaac ctccattcct gcatcgtgac
      301 aggcctgcag ccactgcgcc acttccagcg gatcgccctc ccggcgtacc accctgcctt
      361 ctttattcca taactgcaga caggtgctgc cgtcgagacg caccacaaaa tccccacggc
      421 aggcctgata cgttatgccg ttcagttgca ccgccagatc agtggcagaa gtgccatctg
      481 cgggcagacc tgctgccggg accagcggca gtaccacgac ttctgccgca cgctgttctg
      541 cccggaggcg gtcctgttct gccagcagca gaggctgtgc cacggcccgg ccggcgtccg
      601 tcagctctac tgccagctgc aggttcggag cgcgcagggt acgcagccag ccggcatcct
      661 ccagacgacg gcaggaggcg cgcaggtttg gcccgtagac cggggcttcc ccgcttcgct
      721 ccagcacccg ttccaggtcc cgcgtcagca cgggtcctgg ccgtttttca tcaagggcag
      781 ccagcacaat cagcactctc cgctgcagtg gcgacggtcg gcggctctgg gtcattacct
      841 gtcatcctcc ggccagatga ggtttctgag gcgctgaaag tcggcgtcaa tgagctcctg
      901 tctctttatg gcattcattg aggcatagcc atttatggcc agttcatgcc agaagatcag
      961 cacggctccg gctttggctg actcgcagag ataaatcgcg ccctggctgt ccagattgca
     1021 ttcccggatg cgtttttccg caaaccgggt cagttgttca tagctgagtg aaaacaccct
     1081 ttcttcatcc atcctgcacg ctccatctga taatgttctg acaggtaaaa agaatatcct
     1141 gcatgatttg tgtgctcctg atttctgata atatcagata tggttttatc atcaatgatg
     1201 aaaatcatat ctgatatgat tttatgtgta ttgtttatta tcatatcatt tgcgtctttt
     1261 ctgccgcgca gatccggttt ccggcggtta cggatcttcc tccccctccg cacagcgcca
     1321 caccctgtaa gcacaacatg tggtgccgga ttcagctgct gatgacacta tatgttgtgt
     1381 catctccctg acctgtgatg cgccgcgcag gggcggaaaa cagcgatatg attatttcct
     1441 cagcgtgata cacttccgga aagttgtgat attcaggaaa gtcggatctg acggaaacgg
     1501 ctctccggta atttaacggc gtggttatat ggatgcttgt tatcatggtg atgatgataa
     1561 cggcatgatg ttatcagacg gcgtgacggt aagggcagtg atgatggatg acgttatcgc
     1621 atgaccgtcc ctgcccggaa aagaaaaaag gagtcaccca tgttttttat tgagaatgaa
     1681 ggtcaggctg tcgccagaac ggattactgg cagtctgtac aggcgcaggc cggatatgtc
     1741 tacctcagct ggaatgccgg cgcagccagg ctgcttgtcc cggatgcggc aaaacattta
     1801 ctcagggaga tgcggggggc tgagtacgtc atcatcagta agggagcact gcatggccgc
     1861 gatgcgctgg aactggtatt tgaagacggc agcgatgcgc cgttcgtgat ccacatgctg
     1921 agtgagcagt gcgatcgcct gctccccgaa aacaaccagg gagggggggt tgttgtcacc
     1981 gtccggacgc gtggcggtaa ccagctccgt tatccgggaa agtaccgggt tgtggaaaac
     2041 ctgcccgacg tttccccgtg gagtgaacac tgatatgcag cacctgccag caccgatcca
     2101 ccatgcccgg gatgccgctc agcttcctgt tgccatcgat tatccggcag cgctggcact
     2161 ccgccagatg tcgatggttc atgatgaact gcccaaatac ctgctggccc ctgaagtgag
     2221 cgccctgctc cattacgtcc cggatctgcg ccgcaagatg ctgctggcca cactgtggaa
     2281 caccggtgcg cgcattaatg aagcactggc gctgacgcgg ggggattttt cgctcacgcc
     2341 tccgtatccg tttgtgcagc tggccactct gaagcagcgg acagaaaaag ccgccaggac
     2401 ggcaggaaga atgcccgccg gtcagcagac tcaccggctg gttccgcttt ccgacacctg
     2461 gtacgtcagc cagctgcaga cgatggtggc cacactgaaa atccccatgg aacggcgtaa
     2521 taaacgaaca ggcaggacag agaaagcgcg gatctgggaa gtgacggaca gaacggtcag
     2581 gacctggatt ggggaggcgg ttgccgccgc tgccgctgat ggtgtgacgt tctctgtccc
     2641 ggtcacgcca catacgttcc gccattccta tgcgatgcac atgctgtatg ccggtatacc
     2701 gctgaaggtc ctgcagagcc tgatggggca taagtccatc agctcaacgg aggtctacac
     2761 gaaggtgttt gcactggatg tggctgcacg gcaccgggtg cagtttttga tgcctgagtc
     2821 cgatgcggtg tctatgctca aaagaattcc ataagctctg acttttttaa atccctttcg
     2881 gatatctcat ggatatatta tcggtatttc atattctgtt gacatgcagg accggtatag
     2941 agtaaaaaaa catctattga gtacctcttg gatatccgaa aggtgtctta tggggtcttt
     3001 atgggtatta catgtggatt ctggagatac actatgcctg ttattgctaa tacgcatccc
     3061 aaaggtggtg tgggtaagac aacttcatcc gtaaacattg ttggtgaaat gaagtctgat
     3121 acagttgatc tggatactca taccgggctt tctatcattc tggggctgag acctgaagga
     3181 aaagaaattt ccgtgaaagt acccaaaaca gtggatgagt taatcgaaat tatgactccc
     3241 tacaagaaca gcgataagac gttacttatt gactgtgggg gatttgactc agatcttacc
     3301 cgtactgcga ttgcctttgc agactgcgtt attgtccctt caaaagactc cctgactgaa
     3361 cgtattggtt tgatgcattt tgatggagta ctggacgaaa tcagctctat catggggacc
     3421 gatataactg ctcacctgta tctctgtaaa gttaatccta acaagaagaa attccctaag
     3481 cttgatgcaa tcctgccatc gttcaaacat ctgaagctga tgaagagccg catttctgct
     3541 cgtgcagaat ttgaggatgt cattgagact gggatgggaa tcactgagtc tgttcatggg
     3601 cgctattcgg caggaggtaa ggaagttatt gccctgatcg aagaaatcaa tcatctgatt
     3661 gaaaataaca aacagtaagg tgtgtctatt gggtatccat atagtatcct ttgggctttt
     3721 aatggatatc ttttaagtat ctaacaaaca tcctgggtta tctcatgagc aaagcgaaat
     3781 ttagtgcaca ggctgaaaaa ctgaaaaacc tggtggattc tgccagatta caaacttctt
     3841 cttctgccgt aacagcaaca gccccgtctc agccaaatga aggtgcagag gaagtttcag
     3901 gtcgtcccgt ggctgccagg acgaaggcta agaatcttaa ggccataccg ctgtcgtatt
     3961 ttgaagctca tgcggaactt aaagcgacca gtaaaacatc tctggatttt agctcatata
     4021 ttattgaggc tataagagag aagcttgaac gtgatggtgc aataggtcaa taaactatcg
     4081 gataccttaa gggtatctga tgtgtgtctt ttaaatgcgt tatggggatt cttcacgtac
     4141 ctaaagagtc cccattagac atctttctgt ttatggtgac atgattcgtc accgctttcc
     4201 tttcctctca gtttcaccat ttcattcctt ttcccttaac ctgaaccatg atgtccacga
     4261 tgtttttata ttgcatttat gtgtcacata taaccttcat aatgtttttg tatctatgat
     4321 ttcctc
//
LOCUS       DBANUU010000248         4244 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000248 DBANUU010000000
VERSION     DBANUU010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.253"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            371..529
                     /locus_tag="ACIE68_004614"
                     /pseudo
     CDS             371..529
                     /locus_tag="ACIE68_004614"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            567..854
                     /locus_tag="ACIE68_004615"
     CDS             567..854
                     /locus_tag="ACIE68_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFX5910790.1"
                     /translation="MSPQLIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQ
                     NCRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
     gene            1296..1829
                     /locus_tag="ACIE68_004616"
     CDS             1296..1829
                     /locus_tag="ACIE68_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFX5910791.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITR
                     KIRRADSRRCYRERIFAIFPGYFFILANFDIQPVSALRRHSAFIDFVKFGGEIKPVNK
                     DIVDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLEL
                     VSNAEHHGFMERLVNIP"
     gene            2082..2765
                     /locus_tag="ACIE68_004617"
     CDS             2082..2765
                     /locus_tag="ACIE68_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HFX5910792.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSG
                     ASRHPEFRSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKL
                     QKAGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITP
                     LHRKTMQLLETAELIFSDEGFQVVRGYEHQFGIERRETLFHTLFIGLPSLPEDVWGAT
                     QEQTTITLH"
     gene            2938..3489
                     /locus_tag="ACIE68_004618"
     CDS             2938..3489
                     /locus_tag="ACIE68_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910793.1"
                     /translation="MKKQPLLWMTIAAALLSGCTGHREHHPQVTFTVDTVRTDTTASN
                     PICLAEKYGSLLNERHPDLFAGAQGSISRSAFFNDSLTLTPETLHGMIQNTPCVESLV
                     TSVGPDTFTADVSPGVRMQVSPDIHGAPGNITLRLSTTDQKRKQTVEQSISLRPEQSL
                     VVNGFTGDGAGEKRVVLITPHVR"
     gene            3651..3905
                     /locus_tag="ACIE68_004619"
     CDS             3651..3905
                     /locus_tag="ACIE68_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFX5910794.1"
                     /translation="MPQQHPGRLQVLVVDTHCKRKLFSTKTQTDPDELARRFCTPDNC
                     LVVVLCNNRFLFRLERAPGSHCRWRKGSRSRHQHLQDWLS"
     gene            3948..>4244
                     /locus_tag="ACIE68_004620"
     CDS             3948..>4244
                     /locus_tag="ACIE68_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HFX5910795.1"
                     /translation="MSFLFKLTIAILIVFAILFPILISSDNKLSALFWKIPKDFWVFL
                     IIMYWAIITVVFIESSEQARIRRQQYLQQYKMPNEVHENVVIENSIISRETTDDE"
BASE COUNT         1114 a         1071 c          963 g         1096 t
ORIGIN      
        1 gatcattcga tcgtaaatag aataattctt agatgataaa accatttgtt acacaacccg
       61 ccaattgtgt aacaacagcc tctacaagag catatttgtt catatgttcg tcagaactgc
      121 ggattgtctg gacgagggtc agacaccgtt tcgtgcatat ggaccagcat gttcgaacgc
      181 cacaaagcag aagcatcatg cctggatcgc ttcggcgcgg agcgttaagt tttgacgctt
      241 ttttgtatgt tttttaagct gttagttgac tcggtgcgtt atactaagta ccctttactg
      301 gtcacaggca aaactgtgat cgggcgtagg aacccgcgta gtcatcgtcg gcgcagaagt
      361 taatttcttc atttggcgtc gttttttgcc ataccataaa gacaggaact tgtatttctt
      421 gtcgtcatct atggtagctc aggcggggca ggcattagcc tggccggttt ccgatgacgc
      481 cggtattcct acccccgtct gggctatcac caatgagcgt aggaactcgg tgatagcgtt
      541 tttagttgtc atcggagacc tttgctatgt cacctcaact gatacagaaa ctcccggcca
      601 tcactctgtt ggaaggcatg tttcccgagc tatcaacaaa ccagcttaaa gtctgcgttt
      661 tttacgcaat gggtgttcct tacgacgcca ttgctcagaa ctgtcggctt tctcccgaaa
      721 cggtacgaac ctacctgaag cgaagcctga agaatttaaa tctggaagga tatgatgcac
      781 ttcgctctgc tgtgctgatg cgaactttcg ttttcatgat tagcaataca gctaaagaaa
      841 acgaaaaaat gtgaacaggt gtccccatta aaggggacac tttcatggaa attgcatttt
      901 gcactttaca ggcgatccca aattgataaa cataaacacc aagcacaagt tactttaata
      961 aagcaaatca cttcagacgg tgtcccagtt atttgtatct ggctcatgcc cgatatatgg
     1021 cgatcttact ctgaactgac gtgctgatgt tttatttcgc gtatcacaat actgtgccac
     1081 tgtattgctg atattagtgc ttagccgttc tttatctgtg agttcgccaa atcaccgata
     1141 cagaacgtac tagagtctgc tggttatatg cctgtcagaa ctgcagttta tccagatatg
     1201 ggctggacac tgcttcagac atatgattta gcatattcgc ctgacatcgc aaaatcgtcc
     1261 atcatggatc ggttttgcgc ggagcgttga acttcgtgaa tatagaacac ctgaataacc
     1321 ggaactggta tcttgcgcaa tataatacag ccggcaaaaa cagagaatct ttattctcat
     1381 ggctgaatga gcaaaacgtc gtaccatgga ctccattaat aacaagaaag ataagacggg
     1441 cagactccag gcgctgttac cgtgaaagaa tattcgctat ttttccaggg tactttttta
     1501 ttctggctaa ttttgacatt caaccagtat cggcattacg tcgccatagc gccttcattg
     1561 atttcgtgaa gtttggcggt gaaataaaac ccgtcaataa agatattgtt gatggattaa
     1621 tgaagattta cccggaccct gtgctaaatc cgggagccag ggaagagctg aatgcggctt
     1681 ccagcatatg gctgacaaaa gcacaatatc agtatctcct cagaatggaa aatactctcc
     1741 agcctgagtc gaggatatcc ctgctcctgg aactggtatc aaatgccgaa catcacggct
     1801 ttatggagcg ccttgtaaac atcccatagc cagtatttcc cggccttcgg gccgggcttt
     1861 tttcaattca gagaatattc tggagtgaag cctgcacgaa gccatcccca ccatggactc
     1921 cccctgtctt ttgatggaat cgtgtatttc ttaatggcga aagaaataaa aggcttcacc
     1981 ccagagtatt gattcgtttt atcaaaggcc ggcccgtgcc atcacgtgtg gcggagagag
     2041 cggataccgg tcttcttttc atctcacagt aggacattcg catgaccacg acagaacatc
     2101 ccgtgcgcca gttgctctgg tgtgcactga atgccctgaa aacggcacag gaaaaccaca
     2161 atataaccag tgaaaccgga ttacggcatt acctgcttga atggctttct ggtgccagca
     2221 gacatcctga gttccgtagt ctccctggcg aaatcatggc gttaaaagca ttgaccgaaa
     2281 aagatcgcaa tattcccatc atcggaacgc tgaatacgct ctttctcagc tctgccactg
     2341 tcggggaatg tccgttgttt cgttttcggg ctgcactggg taaattacag aaagcaggct
     2401 ggcgcacata tgtctgccca tggcctgaga gggtgttcaa tgaatccatt gaacgggcct
     2461 gttccgggaa acgtcatctg ctacaactaa gtcgcaccga agagtgtttt ctaccgacag
     2521 gcgatatgag ggcaccagtt acgttgcagc tgatcactcc actacaccgc aaaactatgc
     2581 agcttctgga aacggcagag ttaatattct ccgacgaagg atttcaggtg gtgaggggat
     2641 acgaacatca attcgggatc gaacgcagag aaaccctctt tcacacccta tttatcgggc
     2701 tgcccagcct gccagaagat gtctggggtg caacacagga gcagacgact atcacattac
     2761 actgaggatg tgtaatcccc ggcatacccc gtttccagtc ttcgcagcct gtaccgccct
     2821 tcctgccgtt tccgacattc tttctgtcac tactcgcttg tgccattttt tcgcaagcgg
     2881 cccccatcga catgacggtg cgagtctttc tttttaccct gagaggaaat taacagcatg
     2941 aaaaaacaac cactactctg gatgacgata gccgccgcac tgctgtccgg ctgcacaggc
     3001 cacagggaac atcacccgca ggtgacattc acggtcgata ccgtgcgaac agacacaacg
     3061 gccagcaacc caatctgcct ggcggaaaaa tacggaagcc ttcttaatga gcgccatccg
     3121 gacctgtttg ccggtgctca ggggagtatt tcacgcagtg cattttttaa tgacagcctg
     3181 acgctgacac ctgaaacgct ccacgggatg atacagaata cgccctgtgt tgaatcactg
     3241 gtaacgtccg taggtccgga taccttcact gcagatgtct ctcccggtgt cagaatgcag
     3301 gtatcaccgg atattcacgg tgcgcccgga aatatcactc tgcgactctc gaccacagac
     3361 cagaaacgaa aacagactgt tgaacaaagc atttctctca gaccggaaca gtcccttgtc
     3421 gtgaatggat tcaccggaga cggagctggc gaaaagcgcg tcgttctcat cacacctcac
     3481 gtgcgttaat tcccaacaca acccgtttcc cgtcttcgca gcctgtaccg ccctttctgc
     3541 cgtttccgga attctttctg tcaccactcg cttgtgccgt tttttcgcaa ccggtacgtg
     3601 cagttacgac ggtacagctg cttcttttcc cgttccgaaa ggagtctttg atgccacagc
     3661 aacatcccgg acgactacag gtactggtcg tcgacacgca ctgcaaacgc aagctgtttt
     3721 caacgaaaac acagacagat cccgatgagc ttgcccgacg cttctgcacg ccggataact
     3781 gccttgtggt ggtactatgc aacaaccgat ttctgttccg cctggaacgt gcacccggta
     3841 gccactgccg ctggcgcaag ggtagccgct cccgccatca gcacctgcag gactggctgt
     3901 cgtaaaaaca acaaaaacaa aagacctcgt caaaacagga ggggaccatg tcgtttttat
     3961 ttaaattaac catcgcgatt ttgattgtct ttgcaatcct ttttccgatt ttaatttcat
     4021 cagataacaa actgtccgca cttttttgga agataccaaa agatttctgg gttttcctga
     4081 ttatcatgta ctgggcaatc atcacggtgg tgttcataga aagcagtgaa caggcacgca
     4141 tccgccgcca acaatatttg caacagtaca aaatgccaaa tgaggtacat gaaaatgtgg
     4201 tgatagaaaa cagtattatt tcaagagaaa caacagatga cgaa
//
LOCUS       DBANUU010000249         4184 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000249 DBANUU010000000
VERSION     DBANUU010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.254"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..191)
                     /gene="gpU"
                     /locus_tag="ACIE68_004621"
     CDS             complement(<1..191)
                     /gene="gpU"
                     /locus_tag="ACIE68_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFX5910796.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAIAVYLT
                     GAEYTGEELDSDTWQAELH"
     gene            complement(188..766)
                     /locus_tag="ACIE68_004622"
     CDS             complement(188..766)
                     /locus_tag="ACIE68_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFX5910797.1"
                     /translation="MAIKGLEQAIENLSRISKTAVPGAAAMAINRVASSAILQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIRLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(778..1131)
                     /locus_tag="ACIE68_004623"
     CDS             complement(778..1131)
                     /locus_tag="ACIE68_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFX5910798.1"
                     /translation="MADFDNLFDAAIVRADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(1143..1538)
                     /locus_tag="ACIE68_004624"
     CDS             complement(1143..1538)
                     /locus_tag="ACIE68_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFX5910799.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDGAGQDSSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(1580..2605)
                     /locus_tag="ACIE68_004625"
     CDS             complement(1580..2605)
                     /locus_tag="ACIE68_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFX5910800.1"
                     /translation="MSMYTTAQLLAANEQKFKFEPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSEEN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     THARGLRTYGCIQDADALSEGINASPRYPKNWKTSGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            complement(2661..2993)
                     /locus_tag="ACIE68_004626"
     CDS             complement(2661..2993)
                     /locus_tag="ACIE68_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFX5910801.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(3003..>4184)
                     /locus_tag="ACIE68_004627"
     CDS             complement(3003..>4184)
                     /locus_tag="ACIE68_004627"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFX5910802.1"
                     /translation="VSGDSLTAGEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGT
                     LVSRTRALQPYSGMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIA
                     RVRDIKPVWALANDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQG
                     VEITLIYSGSHKVDGNPYSHLPDDVRETLQSRMDATRQMFAQKVSAYTGLSVQAVLDT
                     EAAVYSGQEAIDAGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATA
                     SQADVTDVVPATEGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVL
                     AETPGMTVETARRILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTP
                     V"
BASE COUNT          853 a         1294 c         1114 g          923 t
ORIGIN      
        1 atatgcagct ccgcctgcca ggtatcactg tccagctctt cgcccgtgta ttcagcgccg
       61 gtgagataaa cggcaattgc cggaaaatcc gcctcatcaa aaacagcggg gcgaccatca
      121 aaaagcgtcg ccccggtgtc atgcttctcc agtgcatcca gtacggctgc acggagttca
      181 gtatgtttca tcgctttatt accattctca gttgatgctg cagcgcatag cccagctctt
      241 tcggaagacg ttcacgccgt atccgttcaa tattctgttt aaacgccgtg gtaagcggca
      301 ccgccatcgg gattttcacc acatcaatgg ggtaacggtt tttcccggcc acacgctgca
      361 tgacatgcca ccggccattt ttcagttgct gaataaacgc gccgggaata cgacggttac
      421 ccaccacaag cacgctgccg ccacctttca gggatgaacg ctgccccttt ttacgacgcc
      481 tgcggcgcga aaggacaacc cgcgcattac ccagcctgat tacgggcaaa tccccccggt
      541 taactttgat tctggcctgc ggatttttga ccgtggccct tttcagcctg gccctttcct
      601 ttaccagttt ccggcgtacc tttgtctcac gggcaacctg tgacgccgac tgcaatatcg
      661 cggatgaagc aacgcggtta atggccattg cggcggcacc aggcaccgcc gttttgctga
      721 tacggctgag gttttcaatg gcctgctcaa gaccttttat ggccatacat ccccctttca
      781 gcggcgacgg ttaacggcag gcggtacgcc ccgcccaagc cagagatgac agcttccacc
      841 atcatccggc gaaacccggt ctatccagaa gttttcctca ccgatggtca gcgtgtctcc
      901 acgccgcagc tgccgcacat catcagtccg gacaaacagg gacgggctgg agccttcaac
      961 gcgcacgccc tgtccggcat agctgatatt ttcagggtca tcaaaaacac cacgtattac
     1021 tgcgccggac tgctcaccgg atgtcatggt ggctgacgtt cccatgtacc cgcgtatcgt
     1081 ttcatcggcg cggacaatgg cagcatcgaa caggttatcg aaatcagcca cagcgcctcc
     1141 cgttattgca ttctggccag gccgcgctct gtcatttcgg ctgccacacc ggcagagaca
     1201 cgaaacgccg ttcccggcag cacaaatgcc acaggttcat cccgcgtggc gtgaagtgca
     1261 tcggtatgca gcgtcaccag tgccacaacc gtgaccagag cagccgtatc agtcacggta
     1321 tccgtctgtg ctgataccac ctcattttca tgtccggtca gcgcattttc cgggctgaca
     1381 gacgagtcct gaccggctcc gtcatccgtg tcatcaagct cctcttccag ctctgccaca
     1441 cggagcgcca gttcttcttt cgtccccgtc aggctgacat cacggttcag ttgctcaccc
     1501 agcgaccgga gacgggcaat cagttcatct ttcgtcatgg actcctccac agagagaaaa
     1561 tggcccctaa gggccatgat tacgccagtt gaacggacac gaactcatca ggatcagcca
     1621 gcagcatcag cggtgctgac tgaatcatgg tgaactcacg cgccggatcg ccggatgtct
     1681 tccagttttt cggataacgg ggagacgcat taataccctc actcaatgca tccgcatcct
     1741 gaatacagcc ataggtgcgc agaccgcgtg catgagtgtt acccagcacc atcgtgttgt
     1801 ccggcaggaa gttctttttg acgccgtttt ccacgtactg tccggaatac acgacgatgg
     1861 ccacatcgcc atacattccc ttataagaca ccgctttacc caggtctttc accgctgtct
     1921 ccagctcgga atgagagcca cgacgggtat ccagcttctc cctgacggct ttgaaggaac
     1981 ggaacagcgc ccagcctttc ggatcgaaca cgatgatatt caccacaccg ctggcgttca
     2041 gcgcgtaggc ttcgatatcg tcggtcgggt catacgtgga cttgtcacgc ttgctccact
     2101 ccgtgccgcc ggactgcgtg atgttattct cctcactgcg gcccatatcc acctcaaccg
     2161 gatcgaaggc ttcaccggtc atggtgtatt tgcccttaag cacggcagaa actgcctgca
     2221 tctcttcgac ctgagcaatg gccagctctt cgtcacgcat gttctgcatg atgatgcgac
     2281 ggcggcggta agccgggtcc gccagattct gcggatcttc atccggcagg cgacgcaggg
     2341 tcatctgcgg attcacttca tgcttcggct tgacatatcc cggcgtaaat tcagaggtgg
     2401 agccgccacg ggaacggata acctcaccgg aaacaatcgg cgaaacgtac agcgccatgt
     2461 ttaccagtcc cggaatttgt gagagataga ctttctccgt ggtgaaggga tagctctcac
     2521 ggaaaaagag acgcagaaac agcggttcaa acttaaattt ctgctcattt gccgccagca
     2581 gttgggcggt tgtgtacatc gacataaaaa aatcccgtaa aaaaagccgc acaggcggcc
     2641 tttagtgatg aagggtaaag ttaaacgatg ctgattgccg ttccggcaaa cgcggtccgt
     2701 tttttcgtct cgtcgctggc agcctccggc cagagcacat cctcataacg gaacgtgccg
     2761 gacttgtaga acgtcagtgt ggtgctggtc tggtcagcag caactgcaag aatgccaacg
     2821 gcagcaccgt cggtggtgcc atcccacgca accagcttac gggtggaggt gtccagcatc
     2881 agcggggtca ttgcaggcgc tttcgcactc aatccgccgg gcgcggttgc ggtatgagcc
     2941 gggtcactgt tgcccagcgg ctggtaatgg gtaaaggttt ctttgctcgt cataaacatc
     3001 ccttacactg gtgtgttcag caaatcgtta acggcatcag atgccgggtt acctgcagcc
     3061 agcggtgccg gtgccccctg catcagacga tccagcgcag tgtcactgcg cgcctgtgca
     3121 ctctgtggtg ctgcagccag aatgcggcgg gccgtttcca cggtcatacc gggggtttct
     3181 gccagcacgc gggcctgttc ttcgcgtccg tgagcctcct cacagttgag gatccccata
     3241 atgcggctgt tttctgccgc aaccgcagcg gtgatctgcg cgttcacgtc cggctgcgcc
     3301 gcgctggcgt tttcgccctc cgtcgctggc accacgtcag taacgtcagc ctgcgaagca
     3361 gtggctgaaa cagttgttga ttgagtctct ttggtcattc gccctcctga gagacgggat
     3421 ttacgtgcat ccagtgcatc acgcataacg gtgatcgcat cggtgctgtt aacaagttca
     3481 tcagccagtc cggcatcaat ggcctcctga ccgctgtaca ctgcagcctc ggtatccagc
     3541 acagcctgca cggacaggcc ggtatatgcc gacaccttct gcgcaaacat ctggcgggtt
     3601 gcgtccatcc gggactgcag tgtctcccgg acgtcatccg gaagatggct gtaggggttg
     3661 ccatccacct tatggctgcc gctgtaaatc agagtgattt ccacaccctg tttctccagc
     3721 gcagcaccgt aattactgtg agccatcatg acgccgatgg agcctgtccg ggcggtctgc
     3781 gtgaccagac gccgggaggc ggcactggca agcagctgac ctgcactgca gttcatgtcg
     3841 ttggccagcg cccataccgg ttttatgtca cgcacacggg cgatgatgtc agcgcagtca
     3901 aatgcccccg ccaccatccc gccgggcgtg tccatatcga gcagaatgcc gtccaccatc
     3961 ggatcgctgg cagcctgttg cagacgggcg ataatgccgt tgtaaccggt catccccgag
     4021 tacggctgca gcgcccgcgt ccggctgacc agcgtgccgg acaccggcag cacggcgatg
     4081 ccgttcatga cctgataact gcgggcctgt cgtggtccgt catcatcacc ggataatgcc
     4141 agcgtcgcga gtgcctctcc ggcagtcagg ctgtcgccgg acac
//
LOCUS       DBANUU010000250         4181 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000250 DBANUU010000000
VERSION     DBANUU010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.255"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..384
                     /locus_tag="ACIE68_004628"
     CDS             <1..384
                     /locus_tag="ACIE68_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFX5910803.1"
                     /translation="QRISFWPQHFGLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTL
                     NNGGAFMAPEPDDDDDETWVLFNVMNGNRTEMSPEAAGIAACLMTYSHHACRTENYAM
                     TVHYYRLRDYALQHPECSAIMRIID"
     gene            400..876
                     /locus_tag="ACIE68_004629"
     CDS             400..876
                     /locus_tag="ACIE68_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFX5910804.1"
                     /translation="MQQLSFLPGAMTPGERSLIQRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            939..1160
                     /locus_tag="ACIE68_004630"
     CDS             939..1160
                     /locus_tag="ACIE68_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFX5910805.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            1240..1614
                     /locus_tag="ACIE68_004631"
     CDS             1240..1614
                     /locus_tag="ACIE68_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFX5910806.1"
                     /translation="MSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYL
                     ADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTLAPATTS"
     gene            1661..2038
                     /locus_tag="ACIE68_004632"
     CDS             1661..2038
                     /locus_tag="ACIE68_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFX5910807.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMARDNYRTVNNITLGKHPGAKQ"
     gene            2035..2523
                     /locus_tag="ACIE68_004633"
     CDS             2035..2523
                     /locus_tag="ACIE68_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFX5910808.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGVTLAELINVVCDNGYS
                     LRVVDESDRTSAERTPPSAALTGIRCSTAHITAKDNAWLYSLSHQTNDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLIVTLIRRYGVSLIHLDACAECLPGLPTF
                     DW"
     gene            2541..2738
                     /locus_tag="ACIE68_004634"
     CDS             2541..2738
                     /locus_tag="ACIE68_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFX5910809.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDRTDSAALLPC
                     IEQAREDIRTLRQLQLLQQNR"
     gene            2835..3677
                     /locus_tag="ACIE68_004635"
     CDS             2835..3677
                     /locus_tag="ACIE68_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFX5910810.1"
                     /translation="MSKITVSRPEVVNGHTDVICSTSICHILAVRKNTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNEIIAKHYPG
                     VLPS"
     gene            3915..4076
                     /gene="rhuM"
                     /locus_tag="ACIE68_004636"
                     /pseudo
     CDS             3915..4076
                     /gene="rhuM"
                     /locus_tag="ACIE68_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774140.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
BASE COUNT         1028 a         1134 c         1025 g          994 t
ORIGIN      
        1 cagcgcatca gcttttggcc gcagcatttt ggcctcattc cacagtgggt gaccctggag
       61 ccccgtgtct tcggctggat ggaccgtctg tgtgaagact actgcggtgg tatctggaat
      121 ctgtacaccc tgaacaacgg tggcgcattt atggcacccg aaccggatga cgatgatgac
      181 gaaacatggg tgctgtttaa cgtcatgaac ggtaaccgca ctgaaatgag cccggaagcc
      241 gccggcattg ccgcctgtct gatgacgtac agtcatcacg cctgtcgtac ggagaattat
      301 gccatgacgg tccattatta tcggttgcgg gattacgccc tgcagcatcc ggaatgcagc
      361 gccattatgc gcatcatcga ctgaatggag agaagcataa tgcaacagct ttcctttctg
      421 cccggagcga tgacgcccgg cgagcgcagc ctcattcagc gggccctgaa aaccctggac
      481 cgtcatcttc atgagcccgg cgtggccttc acctccacgc atgcggcacg ggaatggctg
      541 attctgaaca tggcaggact ggagcgggaa gagttccggg tgctgtatct gaacaaccag
      601 aatcagctga ttgcgggtga aaccctcttc acaggcacca tcaaccgcac agaagtccat
      661 ccccgggagg tgattaaacg tgccctgtac cacaatgccg ctgccgtggt gctggcgcac
      721 aatcacccgt ccggtgaagt cacccccagt aaggcagacc ggcttatcac tgaacgtctg
      781 gtacaggcgc tggccctggt ggatatccgg gtgccggacc atctgatagt cggtggcaac
      841 caggtattct cctttgccga acacggtctg ctttaacccg tcacaaccac atcacacctg
      901 ttttcacttt tatcttctgt cttcagaggt atcccattat gaaaatcatc acccgtggtg
      961 aagccatgcg tattcaccaa cagcatcctg catcccgtct ttttccgttc tgtaccggta
     1021 aataccgctg gcacggtagc acggatacgt ataccggccg tgaagtgcag gatattcccg
     1081 gtgtgctggc cgtgtttgct gaacgccgta aggacagttt tggtccgtat gtccgtctga
     1141 tgagcgttac cctgaactga atcagaaagg gcattctgat gagccgaatt atcgccacca
     1201 tcggagcatt cttaaccgat tttctgtgag gattttatcg tgtcagacac acaccccggg
     1261 acaacgcatc ccgacaacaa caacgaccgc ccctggtggg ggctcccctg caccgtgacg
     1321 ccctgtttcg gggcacgtct ggtgcaggag ggcaatcagt tgcattacct tgccgaccgc
     1381 gccggtatca gggggcagtt tagcgacgcg gatgcgtatc atctggacca ggcctttccg
     1441 ctgctgatga aacaactgga actcatgctc accagcggtg aactgaatcc ccgccatcag
     1501 cataccgtca cgctgtatgc cagagggctg acctgcgaag ccgacaccct cggcagctgt
     1561 ggttacgttt atatggctgt ttatccgaca ctggcacccg caaccacctc ataaccccga
     1621 atatctcacc tttagcacta ccagttacag agaacttaag atgaaaacat tacctgacac
     1681 acacgtacgg gaggcatcgc gctgcccgtc tcccgtcacc atctggcaga cactgctcac
     1741 ccgactgttg gaccagcatt acggcctcac actgaatgac acaccgttcg ctgatgaacg
     1801 tgtgattgag cagcatattg aggcaggcat ttcactgtgc gatgcggtga actttctcgt
     1861 tgaaaaatac gcactggtac gtaccgacca gccgggattc agcgcctgta cccgttctca
     1921 gttaataaac agtattgata tcctccgggc ccgccgggca accggcctga tggcccgcga
     1981 caattacaga acggtaaata acattaccct gggtaaacat ccgggggcga aacaatgaaa
     2041 ctggccctga cgctggaagc cgacagcatt aacgtgcagg cactgaacat ggggcgcatt
     2101 gtcgttgacg tcgatggtgt tactctcgct gaactgatta acgtggtctg cgataacggt
     2161 tactcccttc gtgttgttga tgaatctgac cggacatcag cagagcgcac gccaccatct
     2221 gcggccctta ccggcatacg ctgcagtacc gcgcatatca cggcaaagga caacgcctgg
     2281 ctgtactcgc tgtcacatca gacgaatgac ttcggcgaat cagaatggat tcattttacc
     2341 ggtagcggat atctgttacg taccgatgcg tggtcgtacc cggttctgcg acttaaacgc
     2401 ctaggcctgt caaaaacgtt tcgtcgtctg atcgtcacgc tcattcgccg ttatggcgtc
     2461 agtctcattc atctggatgc ctgcgctgaa tgtctgccgg gtctgcccac tttcgactgg
     2521 taatcaggga aattcacaac atgaaatcat taacgacaga aacggcgctg gatattctga
     2581 ttgcgtggct gcaggacaat atcgactgtg aatccgggat tatcttcgac aacgatgagg
     2641 atagaacgga ttcagcagca ctgttgccct gcattgaaca ggctagggaa gatatccgta
     2701 ccctgcgcca actacagctt ctgcaacaga accggtgagt cttactcatc atctcactca
     2761 ccagacttca ttccactgac accagcctga acagggctgg cgttttcatt tatctgcaaa
     2821 aaggaatatc gattatgtct aaaatcacag tctcccgtcc ggaagtggtc aacgggcata
     2881 cggacgttat ctgttccacc tcaatctgcc acattctggc tgtacgaaag aatacactgc
     2941 tgcaaatcga cacacttatc cggcaactgg ctgaaatctc ggtgctgacg gaaagcatcg
     3001 gaggtaaaac tgccccggac tgggccatga aacaggattt tcgctgcgga tgctggctca
     3061 tggagaaacc tgaaacggca atgaaagcca ttacccggaa tcttgaccgc gaaatctggc
     3121 gtgacctgat gcaacgttca gggatgcttt ccttaatgga tgcacaggcc cgtgatacat
     3181 ggtaccgatc actggaatac gataattttc cggaaatcag tgaagcgaac attctgagca
     3241 catttgaaca actgcaccag aacaaagatg aagtgtttga gcggggggtg atcaacgtct
     3301 tcaggggact gagctggaat tacaaaacca attgcccctg caagtttggc agtaaaatta
     3361 tcgtcaacaa cctggtaagg tgggacagat gggggtttca tcttatcacc gggcaacagg
     3421 ccgatcgact tgccgatctg gaaagaatgc tgcacctgtt cagcggcaaa ccgatccccg
     3481 acaaccggga aaacatcacc attcatctgg atgaccacat ccagtctgtt caaggtaaag
     3541 aggactatga agatgagatg ttcagcatca gatactttaa gaagggctcc gcacacatca
     3601 cgttcaggaa accagaactg gttgacaggc tgaatgagat tattgcgaaa cactatccgg
     3661 gagtgttacc ttcataagaa agctctaata attgattcga ttgtgtattt tagcctcatt
     3721 cccttacgca aagtcatttg ggggttctta tgggggttac tcatttatcc aacacgggtt
     3781 aatcatttaa aatcaaaaca ttaacactca tattcgactc ctgtgatctt ccgccaaaat
     3841 gcctctcctg acgttctcca accccttgta atactgtcaa tccagatatt ttctactgct
     3901 caatattcaa tcggcttcag gaatacctgg tctaaggttt tgtgatggac gatgagcggc
     3961 tgaagaatcc gcctgtcggt tcttctgtcc tcgaacgcat ccgcgatatt cacgccagta
     4021 tgcgccgtgt ttatttgcag gtgatttgcc cccctgctga ctctcagcca actgtatcgc
     4081 cattaaggtg aagatgttgt tttactcgcc ccagaacacc cttttttata cgactattct
     4141 atttctttaa ccgctggcga aaaaaactga ctataaactt c
//
LOCUS       DBANUU010000251         4150 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000251 DBANUU010000000
VERSION     DBANUU010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.256"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..522
                     /locus_tag="ACIE68_004637"
     CDS             <1..522
                     /locus_tag="ACIE68_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFX5910811.1"
                     /translation="LFVEKGYQAVSIDEISGKALVTKGAFYHHFKNKKQLLSACYKQQ
                     LIMIDAYITTKTDLTNGWSALESIFEHYLDYIIDNNKNLIPIQEVMPIIGWNELEKIS
                     LEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLNGWFMHIAIHAKNLKELADKKGQFI
                     AIYRGFLLSLKDK"
     gene            complement(635..1840)
                     /gene="tet(B)"
                     /locus_tag="ACIE68_004638"
     CDS             complement(635..1840)
                     /gene="tet(B)"
                     /locus_tag="ACIE68_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFX5910812.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            1922..2545
                     /gene="tetR(B)"
                     /locus_tag="ACIE68_004639"
     CDS             1922..2545
                     /gene="tetR(B)"
                     /locus_tag="ACIE68_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFX5910813.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            complement(2523..3209)
                     /locus_tag="ACIE68_004640"
     CDS             complement(2523..3209)
                     /locus_tag="ACIE68_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFX5910814.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(3217..3603)
                     /locus_tag="ACIE68_004641"
     CDS             complement(3217..3603)
                     /locus_tag="ACIE68_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HFX5910815.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            complement(3596..3916)
                     /locus_tag="ACIE68_004642"
     CDS             complement(3596..3916)
                     /locus_tag="ACIE68_004642"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HFX5910816.1"
                     /translation="MIAVIFEVQIQPDQQTRYLTLAEELRPLLSHVAGFISIERFQSL
                     ATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGRESIFDFYKISIAHITREYSFKKD
                     KDNV"
BASE COUNT         1335 a          880 c          750 g         1185 t
ORIGIN      
        1 ttgtttgttg aaaaaggcta tcaagctgtt tcaatagatg agatctcggg aaaagcgttg
       61 gtgaccaaag gtgcctttta tcatcacttt aaaaataaaa aacaattact cagtgcctgt
      121 tataagcagc aattaattat gattgatgcc tacatcacaa caaaaactga tttaacaaat
      181 ggttggtctg ccttagaaag tatatttgaa cattatcttg attatattat tgataataat
      241 aaaaacctta tccctatcca agaagtgatg cctatcattg gttggaatga acttgaaaaa
      301 attagccttg aatacattac tggtaaggta aacgccattg tcagcaaatt gatccaagag
      361 aaccaactta aagcttatga tgatgatgtg cttaaaaact tactcaatgg ctggtttatg
      421 catatcgcaa tacatgcgaa aaacctaaaa gagcttgccg ataaaaaagg ccaatttatt
      481 gctatttacc gcggcttttt attgagcttg aaagataaat aaaatagata ggttttattt
      541 gaagctaaat cttctttatc gtaaaaaatg ccctcttggg ttatcaagag ggtcattata
      601 tttcgcggaa taacatcatt tggtgacgaa ataactaagc acttgtctcc tgtttactcc
      661 cctgagcttg aggggttaac atgaaggtca tcgatagcag gataataata cagtaaaacg
      721 ctaaaccaat aatccaaatc cagccatccc aaattggtag tgaatgatta taaataacag
      781 caaacagtaa tgggccaata acaccggttg cattggtaag gctcaccaat aatccctgta
      841 aagcaccttg ctgatgactc tttgtttgga tagacatcac tccctgtaat gcaggtaaag
      901 cgatcccacc accagccaat aaaattaaaa cagggaaaac taaccaacct tcagatataa
      961 acgctaaaaa ggcaaatgca ctactatctg caataaatcc gagcagtact gccgtttttt
     1021 cgccccattt agtggctatt cttcctgcca caaaggcttg gaatactgag tgtaaaagac
     1081 caagacccgc taatgaaaag ccaaccatca tgctattcca tccaaaacga ttttcggtaa
     1141 atagcaccca caccgttgcg ggaatttggc ctatcaattg cgctgaaaaa taaataatca
     1201 acaaaatggg catcgtttta aataaagtga tgtataccga attcgattgc gtctcaaccc
     1261 ctacttcggt atctgtatta tcacgtgtat ttttggtttc acggaaccaa aacataacca
     1321 caaggaaagc gacaatattt agcaacgcag cgataaaaaa gggactatgc ggtgaaatct
     1381 ctcctgcaaa accaccaata ataggccccg ctattaaacc aagcccaaaa cttgccccta
     1441 accaaccgaa ccacttcacg cgttgagaag ctgaggtggt atcggcaatg accgatgccg
     1501 cgacagcccc agtagctcct gtgatccctg aaagcaaacg gcctaaatac agcatccaaa
     1561 gcgcacttga aaaagccagc aataagtaat ccagcgatgc gcctattaat gacaacaaca
     1621 gcactgggcg ccgaccaaat cggtcagaca tttttccaag ccaaggagca aagataacct
     1681 gcattaacgc ataaagtgca agcaatacgc caaagtggtt agcgatatct tccgaagcaa
     1741 taaattcacg taataacgtt ggcaagactg gcatgataag gccaatcccc atggcatcga
     1801 gtaacgtaat taccaatgcg atctttgtcg aactattcat ttcacttttc tctatcactg
     1861 atagggagtg gtaaaataac tctatcaatg atagagtgtc aacaaaaatt aggaattaat
     1921 gatgtctaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
     1981 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
     2041 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
     2101 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
     2161 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
     2221 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
     2281 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
     2341 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
     2401 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
     2461 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
     2521 acttaaatgt gaaagtgggt cttaaaagca gcataacctt tttccgtgat ggtaacttca
     2581 cggtaaccaa gatgtcgagt taaccaccct ttagattcat aaagcgaaaa taatgcggct
     2641 ccaacgtacc cacctaaatg gaaacggcgt tcactccaat ctaaacacgc acaacagatt
     2701 ttacgtgaat gtttggaagg aacgtcaatt cccatttcat gaaaatattg aataccactt
     2761 aatgtgatca ttgaaccatt ttcagtgatc cattgctgtt gacaaaggga atcatagatc
     2821 ttaacggcaa cttcgccagc taaatgatca tagcaagtac gtgcttttcg taaatgcact
     2881 ggcgtggaaa ctttggcatg tacgccatgg tttaaggaga tccccatcat actttccatc
     2941 aattcagcaa tatcttttcc tgctagccga aaataacgat gcttgccttg agctactact
     3001 gtgattagct ggcaatctaa taatttagat aaatgactgc tcgccgttga agctgatata
     3061 ttcgccacag aacttagctc agtggccgtc caagctcgcc catccatcaa agcactgagt
     3121 attttaactc gtgaaatgtc agacatagcc gcccctatcg cggctattga ggactcaaag
     3181 gtaacctctt ttcgtattaa attagccatc gcaagttcac tttattgccc aagggagcgt
     3241 aacagatgca gccatactat cattgtccgt tattaatatc agttggttag catggtcact
     3301 gtattgcact aaaatattaa tgttattctc cgccaatact cgtgctattt cgccaagttc
     3361 ccccggtttt tcctgtttta acttacgaat taatggtgtc cggatcgcaa gtactaacag
     3421 tccagcttgc tctagcgcta ttttagcttc ctttccttgt tcaacaagaa aatgagcatg
     3481 gcattcatca ccaaccgtaa atatccctcc cccttccaat ccaatacctt tattacctaa
     3541 tgtttttcct agtaatgcta attgtcctat ttgattatct aaaacaacgt gaacatcaaa
     3601 cattatcctt gtccttttta aatgaatatt cgcgagtaat atgagcaata ctaattttgt
     3661 aaaaatcaaa tattgactct cgcccttctt gttgagcttt cgcatgtaaa acatgatttt
     3721 tccattgcag aactgcgtat tcgttttccc accaagatag cgataacatt tttccttctg
     3781 tagctagact ttgaaaacgt tcaattgaaa taaaaccagc tacatgactt aatagtggtc
     3841 ttaactcctc agctaaagtc aaatagcgag tttgttggtc gggttgtatt tgcacctcaa
     3901 atattacagc aatcatattt ttctccgtta ttaatcaaca aacagagtaa tccacccgaa
     3961 aattaacact tcgataagca acgaaatata gaaaggtcat gctgctgttt tatgttgtat
     4021 atcacacttc tttaaattgc tcggcctgcg cttatcatgt tctccttctc ttataatttt
     4081 gtttattatt tatacttttt tattttttgc tatcaccgaa aatagtgcgg atcccgcatg
     4141 gtatttaggt
//
LOCUS       DBANUU010000252         4141 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000252 DBANUU010000000
VERSION     DBANUU010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.257"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..227
                     /locus_tag="ACIE68_004643"
     CDS             <1..227
                     /locus_tag="ACIE68_004643"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910817.1"
                     /translation="ALHYLTQESATTYIPSVVGLFEKLAQYLKLVRDKLRDEQSQLVS
                     KLPELPAVYQRCHIATTYSALSTATTENTQ"
     gene            224..1015
                     /locus_tag="ACIE68_004644"
     CDS             224..1015
                     /locus_tag="ACIE68_004644"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910818.1"
                     /translation="MMTNWSEADEQALSALNERLNVKDPAENARNKLAVQTSVNQIIT
                     QLEQTAKAFSQSNIDCLRALRADAISKRRIALDSERIKDDSLEGIGSPVWRAMWEAAR
                     DYSRTPYPDKAFSATDEARCVLCNQALSAEAQQRLNNLEAFVQSRLESDAATTECLYV
                     QAIGSITIPPSMAEIATLCASAAIQEEWNKYLTGIWSAASLSRQALTEHEATQTAVPL
                     ADLTEAITNLRTYRDRLQGEADQFQRDAAGFDRAKALQNKNEAEA"
     gene            1189..2037
                     /locus_tag="ACIE68_004645"
     CDS             1189..2037
                     /locus_tag="ACIE68_004645"
                     /inference="COORDINATES: protein motif:HMM:NF024565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFX5910819.1"
                     /translation="MKVLGAPSLQVELVKIRTENAKVLHQLQLKGAQRDKLHNVLSEG
                     ERRIISLAAFLADVTDRPGSAPFIFDDPISSLDHEFEWQVAKRLVELAKSRQVIILTH
                     RLSLYGVIEDLARKEGDPWKKQHHKSICIESFDGAAGHPADQEVWNANTKSANNILLT
                     RLDSAYKSGQEHGAIAFRGLAQGVCSDFRKLIERSVEEDSFNGVVIRHRRGIQTDGRL
                     PYVQDITAEDCKLIESLMTKYSCYEHSQSTEMPASIPEYKELRADISTLKEWRDQIKT
                     RRTLMK"
     gene            complement(2588..3319)
                     /locus_tag="ACIE68_004646"
     CDS             complement(2588..3319)
                     /locus_tag="ACIE68_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFX5910820.1"
                     /translation="MNQPIHNAYWLSRFESILNSALAQHRAISLIRVDLRFPEYMPAT
                     IMDPDPDSAVISRFSESLKAKIQAYQRHQRRAGKRVHATTLHYFWCREFGKKYGRKHY
                     HVILLLNKDTWCSPGDFTVPSSLATLIQLAWCSALHLEPWLGNGLVHFARWTPSRKPA
                     SADAPPSSDDTPLPGGCSDTRKASDKKSGGSAVLWVTRGDEEAVQKALERARYLVKNE
                     TKLHDGSGQRNYGCSRGSGRLLDGR"
BASE COUNT         1237 a          957 c          964 g          983 t
ORIGIN      
        1 aagctctgca ttatctcacc caagagagtg ccactaccta tataccgagt gtggtggggc
       61 tttttgaaaa actggcccag tacctgaaac tggtacgcga taagctaagg gatgaacagt
      121 ctcagctagt cagcaaactt cccgaactac ctgctgttta tcagcgttgt catattgcca
      181 ctacctatag tgcactaagt acagcaacta ccgaaaatac gcagtgatga caaactggtc
      241 tgaggcggat gagcaagcgc ttagtgcctt gaatgaacgt ctgaacgtta aagatccggc
      301 tgagaacgcc cgcaacaagc ttgcggttca aacatctgtt aatcagatca tcactcaact
      361 tgagcagacg gcaaaggctt ttagccagtc aaatatcgac tgtcttcggg cgttacgtgc
      421 tgatgctatc agcaagcgca gaattgcgct tgatagtgaa cgaataaaag atgactcact
      481 ggagggcatt ggctcacctg tatggcgtgc catgtgggaa gctgctcgcg actattcaag
      541 gacgccctat ccagacaagg ctttttcggc aacagatgag gcccgttgtg ttttatgcaa
      601 tcaggctcta tccgcagaag ctcagcagcg tctgaacaat cttgaggctt tcgtgcaatc
      661 acgtcttgag agcgatgctg ccacaactga gtgtctgtat gttcaggcaa tcggctcgat
      721 aactattcct ccctcaatgg ccgaaattgc taccctttgt gcaagcgctg cgattcagga
      781 agaatggaat aaatacctaa cgggaatatg gagtgccgca tccctcagtc gtcaggcgct
      841 caccgagcat gaagctaccc agacagcagt tcctttggct gatttgaccg aagctatcac
      901 gaatttacgt acttaccgcg accggcttca gggagaagcc gaccagtttc agagagatgc
      961 agcaggattt gacagggcca aagctttgca gaataaaaac gaagccgaag cttgacggtg
     1021 gttatctggt caagccgagg ctatcagagc cgaagttgaa cgcttaaaaa aagaagtgaa
     1081 tttgatgaat ggatatcact tgccgcttcc cgtgcattat caactcaatc tgctgatatt
     1141 actcagaaag ttgtcactga agcctatgcc gccagattca accgtgagct gaaagttctt
     1201 ggtgctccta gcttgcaggt tgagcttgtg aaaataagga cagaaaatgc gaaagttttg
     1261 catcagctac agctaaaagg agcgcaacgg gacaagttgc acaatgttct gagtgaagga
     1321 gaacgtagga ttatctcact ggctgcattt ttagccgatg tcactgatcg gccgggatct
     1381 gctcccttca tttttgatga ccctatttca tctctggacc acgaatttga gtggcaagtt
     1441 gctaaacgcc tcgttgaact cgctaaatcg cgtcaggtga ttattttaac gcacagactt
     1501 tccctttacg gggtaattga agatttggca agaaaagagg gagacccatg gaaaaagcag
     1561 caccataaat cgatatgtat tgaatcattt gatggagctg ccggtcatcc tgccgatcag
     1621 gaggtctgga acgcgaatac caaatcagcg aataacattc tcctgactag gctggattca
     1681 gcctacaaat ctggacaaga acatggcgca atagctttcc gtgggctagc tcagggcgtt
     1741 tgcagtgatt ttcgtaaact cattgagcgc tctgtcgagg aggattcatt taacggggta
     1801 gttatacgtc atagaagagg aatccagact gacggaaggc ttccgtatgt acaggatata
     1861 actgcagaag actgtaaatt gattgaaagc ctcatgacta aatacagctg ttatgagcac
     1921 agccagtcga cggaaatgcc tgcttctata ccagagtata aggagctgcg agcggatata
     1981 agcacgttaa aagaatggcg tgatcagatc aaaacaaggc gcactcttat gaaataaccc
     2041 ctcattgaat ccagtatctt tattgtctta gtaaaaatct ggggtcagta gaaagtatca
     2101 tttccgtgct ggatggagaa tctcatcggt aaatccggat ggtatgcttg aagaggcctt
     2161 tctgccagca gttacttggc tagctaagcc ataccagatt gggcggcatt gcggtagctg
     2221 gaagataata tcagtgaata ccgggggact taagtacctt cccggtatcg atgcagagca
     2281 taatagtggg aacttccgct tctggcacaa agcagactat gagaagagag atgctataac
     2341 atttgagtcc caccaatcga tctgttcatt tctatgaatg gcagtaaatg caaataacca
     2401 taacaaccaa acgtttttcg acttatggtc gatgtcgcaa agagtgaggt gtggaaagca
     2461 agcgtgtgaa cggaagtaga tggctatcca ttgcattact tcaggatgac ttttcagaaa
     2521 acgagctaag ggcgtcaacc aacgccctgt agcataagct caggtaccgg atgccgtttt
     2581 acaggcttca cctgccatcc agaagacgcc ccgacccacg actgcaacca taattacgtt
     2641 gacctgaacc gtcatgcagt ttcgtttcat ttttcacgag ataacgggct ctctccaggg
     2701 ctttctgcac cgcttcctcg tcaccacgcg tgacccagag cacagcggat ccaccagact
     2761 ttttgtcaga agccttccgg gtatcagaac acccaccagg caaaggcgtg tcatcggaag
     2821 aagggggggc atcagccgat gctggtttac gggaaggagt ccacctggca aaatgaacca
     2881 gtccattacc cagccagggc tcaagatgca gggcgctgca ccatgccagc tggatcagcg
     2941 tcgccagcga agaaggaacg gtgaaatccc ctggcgagca ccaggtatct ttgttgagca
     3001 gtaatatcac gtgataatgt ttcctgccat atttcttgcc gaactcccgg caccagaaat
     3061 aatgcagggt ggttgcatgc acacgcttac cggcacgtcg ctgatgtctc tggtaagcct
     3121 ggattttggc tttcagggat tcggaaaaac gggagatcac cgcagagtcc ggatcgggat
     3181 ccatgatggt ggcaggcata tactcaggga aacgtaaatc cacccggatt aacgagatgg
     3241 cacggtgctg cgccagggca ctgttgagaa tgctttcaaa acgggataac cagtaagcat
     3301 tgtgtattgg ttggttcatg atgagagatc tccgttgatt tggattgact tcacatcatt
     3361 aatcaccgtg gtaaaacttt actgcatccc gccattgagc aacaccaatg gctcctgatt
     3421 aaaatggaat accctacaaa cgaaaagaga atccaactga cacacagggt aaaaacatct
     3481 ctgcaaaaca taaaataaca gcatgaatgg atttaaaaac gatatggacg acgagaaaag
     3541 tcagaagggg aacggatcca caggacattc aaacaaaacg tcacaaacaa aacgtcagaa
     3601 actcaatcag aacgctttct ggcgctgtga atcaacaaca atgccaaaac ctacagctta
     3661 accacattac tcaatcacag atgcgaaatg gcacacttaa aacctgttta cagataatcc
     3721 cgccgcaaga acaaaaaaca ccaacatcag aatcgatttt cttatcctga aaacgttact
     3781 tttcactacg ccccataaaa cgattaacta tcgataacgt aaaccgcatc tgaatcaaaa
     3841 caccagaacc aatcatcacg ctttttctga aaagagcctt gcgctaagaa aaaacaccag
     3901 ggccagttaa tcatctcatc aatcttatcg ttacaaaaac atccagaaag tcgtttaaca
     3961 aaatcatcgt aagcacacag taaatcacgc taggttatct cagtcggatt taagttttta
     4021 cgtaaagata cttttctcat gctaacgtcg aaactcgaaa tctcaggaaa ggttaaacgt
     4081 aacatcagta aaatcgaata gcagattgat tgcttaaaca gaaaggttcg ttttacaaca
     4141 g
//
LOCUS       DBANUU010000253         4134 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000253 DBANUU010000000
VERSION     DBANUU010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.258"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..428)
                     /locus_tag="ACIE68_004647"
     CDS             complement(<1..428)
                     /locus_tag="ACIE68_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFX5910821.1"
                     /translation="MRKVCAAILSAAICLVVSGAPAWASEHQSTLSAGYLHAPTNVPG
                     SDDLNGINVKYRYEFTGTLGMVTSFSYAEDKNRQLTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYLRVTDNKGKTH"
     gene            complement(499..3912)
                     /locus_tag="ACIE68_004648"
     CDS             complement(499..3912)
                     /locus_tag="ACIE68_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFX5910822.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQHCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMSDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNNQTRTLTLD
                     REITQPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSIWGMKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDLSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETAYRFRQ
                     LALGRYTLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKALLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGT
                     LNNVTINENCTIKGMLEANQVRGDFVKTVSKSFPKKAGTWGNTETPDGTVTVTISDDH
                     NFDRQIIIPPIIFNGIAYDDPGSGDNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGS
                     YSAVIDMPSGRGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
     gene            complement(3973..>4134)
                     /locus_tag="ACIE68_004649"
     CDS             complement(3973..>4134)
                     /locus_tag="ACIE68_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFX5910823.1"
                     /translation="KQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGD
                     GGQVVVIGR"
BASE COUNT          849 a         1286 c         1020 g          979 t
ORIGIN      
        1 tcgtgcgttt tccccttgtt gtcagttacg cggagataat ccccggaaaa agtcgaaaca
       61 cggctgtaag ccatacccgc catcgcatac gcgctgaacc attcattcac gcgcacagac
      121 ggccccgcca tcacgctgaa ccagcggtta cgcacggaat cttcatgcca gcgggtatcg
      181 ctgtaatggg tcaactggcg attcttgtct tctgcatagc tgaatgacgt caccatcccc
      241 agcgtgcccg taaactcata acggtatttc acgttaatcc cgttaagatc atcgctgccg
      301 ggaacgttcg tcggggcatg aagatacccc gcgctcagcg tggactgatg ttcagacgcc
      361 catgcaggcg caccggatac gaccagacag atggctgcgg acaaaatggc tgcacaaact
      421 ttacgcataa ttacctctcg cttttctgca ataaaaaagg cgtcattcct gacgcccttt
      481 attggggtta taacaatttc aacgaatact gatgccggaa gcagcctttt tggtcacaat
      541 caccgtacag tcggtgatat tgcctgcccc ctgattgcct ttctggaaaa tcttaaactc
      601 cagagtgacg cttcccctgc cactcggcat atcaataacc gcactgtaac taccgggaat
      661 ggccccttta gtttctctgg atgcgattaa tacgccgttt ttgcgaactt caaaaccata
      721 ccccgtgtac cttgtacctc ccgggttatc accacttccc ggatcgtcat acgctatacc
      781 gttaaagata atgggcggaa taatgatttg acggtcaaag ttatgatcat cgctgatggt
      841 gactgtaacc gtcccgtctg gtgtttccgt gttaccccac gtaccagcct ttttcgggaa
      901 tgatttggat actgttttaa caaaatcacc tctgacctga ttcgcctcca gcatgccctt
      961 aatcgtacag ttttcattta tcgtgacgtt gttgagcgtc ccggagttcg cattcacact
     1021 gccactgata tccgcatttt tagcggtcag ctttccgtct gatgtcaggg aaaataccgg
     1081 aggactgcca ccgctggtaa tggtgggtgc cgtcaggcgt ttcaggaaca cgtcgttcat
     1141 gaatatctga ttgccctgcg ccacaaacat cggcgtttca ttcccgtttg ccgggtcaat
     1201 aaatgcgata cggttagcgg caaccagaaa ctggctcagc ttgccttcct ccgtgtcctc
     1261 catgctgagg ccaatacccg cgacataatg tttgccgtct ttggtctgct caattttgac
     1321 gccccacatg gcattccatt tatcgttagc gtccttccac tctttcgaaa actcatccag
     1381 tctgctggcg ttatcttccg tcagatcaac tttttccagc agcgctttac cgagatggga
     1441 ttcggttatc ttgcctttga aaaaatccag gtaaccttcc gcatcatcgc tcgcccgacc
     1501 gacggcctcc acgaatgccg atttgccaac ggtgttcaca ctgcggatat aaaagtaata
     1561 atcatggccc ggtttgatat tgatactggc ggctatccag tacagccccg tgccaaggta
     1621 gcgagctgtg gtttcaacct gcctgatatc ggtaatccgc gtttccgaga accagaactc
     1681 aaactgtacc gtcggatcat aaaccgcaag atgcggcgtg gcagttatct gaaaatagcc
     1741 cggcgtcagc tcaatccgcg acggcgctgc cggggcggca atccggaacg ataccgatgc
     1801 cggatcgccc tgctgtcccc acgcatttac cgcccggacc gtcagcgtgt aacgccccag
     1861 cgccagttgc ctgaagcggt atgcggtttc cgtcgtccgg gccgtgctga ccagccgctc
     1921 actgccgtca tccgctgcca cggtcaggcg aagcaggaaa ctcacgccct tcaccacctt
     1981 cggcgtgtcc cagcgggcca gtacctgata ttccccgctg tctgcggtga cttcggcagt
     2041 caggtgctgc accgcgggcg gcgtgacgcc attcaccgtg ccgctcaggt cgccgtcaaa
     2101 gtgcgccccg ttatccacga tggcttcttt ttcgggtaca tgctgcacgg cggtgatggc
     2161 atacgtgccg tcgtcgttct cacggatact cacacagcgg aacaggcgct ggcgcaacgt
     2221 cggcagcttc atcccccata tgctgtatcc ggcaacgccg tcaggaacac ggctcacttt
     2281 caccttcaca ccgtcggtga cggactgaac ctccacgctg accggattgc cacttccgtc
     2341 aaccaggctt atcagcgtgg taccggagga tggctgcgtg atttcacggt cgagcgtcag
     2401 cgtccgggtc tggttgttca ccgccagcac gcgcccgccg gtgctgatac cggcatagtc
     2461 atcatcgcag atttcaatga catcgcccgg tacatggcga agcccttcgg cacccacgct
     2521 gaagtcaacg gtctgcgtct ccagcagttc cgttttaatc agccacagcc cggcgcggtg
     2581 tgcctgcccc cggctggtac agccaaaggc atccatcttc gtgacgttac gaccgtaacg
     2641 ggcaatggcc tgcgtgtcct ccactagctc tgtcgccgtc tcccagccgt tatccgggtc
     2701 aatccagttc acctcaacgg cattatggcg gtccttcagg gcgctgaaac tgtagcggaa
     2761 cggcgcgcca tcatccgaca tcaccacatt actgcggtta taggtccaca ccttatccga
     2821 cggtcggtcc tgcacgaacg tcagcgtctg cccgttccat accggcatac agcgcatcgc
     2881 cgagcagaaa tcactgagca catcccacgc cttgcgctgt gtggtcaggt acgcattaca
     2941 ggtgatgcgc ggctccgtgc cgccaaaacc gtccggcacc gactgatcgc aatgctggcc
     3001 gatgacatac agcgcccatt tgtccacatc cgccgcacca agacgtttcc ccatgccgta
     3061 gcgcggatgg gtcagcatat cccacagaca ccaggccatg ttgttgctgt atgctggctt
     3121 aaacgttccg tcccagatac cgctgtattg ccgcgtctgc gggttatagt tcgacggcac
     3181 ctgcagaatg cgcccgcgaa gatgataatt acggctcacc tgctggctgc cgaactgctc
     3241 cgagtccacc tgcacgccga ccagtgccgt gttcgggtag cactgtttca catcgatgat
     3301 ttcggtgtat gacgaccaga gcgttttgtt ctgcagctgg tctgtggtgc tgtccggcgt
     3361 catcctgcgc atccgtatat tgaacgggcg cggcggcagg ttacccacca ccaccgaggc
     3421 cagatactgc gaggtggttt tgcccttaat ggtgatgtct ttttccgtca cccagccacc
     3481 attacgctgt atctgaacca gcaggcggac ttccgacgga ttcctgtccc cctttgaggt
     3541 ggtttccacc agtgcctgca cgccgaaggt aaaacgcagt cggtcaatgt ttgcagacgt
     3601 aatggtgcgg gtgatcggcg tgtcatattt cacttccgta cccagcaccg tctcggagcc
     3661 ggaggattca aatccctccg gcggtgactg ctcctgctca ccggcccgga acaccaccgt
     3721 gacgccggat atattggtat tcccctcact gtccagcacc ggcgtactgt tcagcagcac
     3781 gctttttaat ccatccaccg gaccttcaac cggcccttcg ctgatggcat cgatcacact
     3841 cagcagctgc gtggacttca ggttgtcctt cgcttcgcgc ggggtatgcc ccttactgct
     3901 gcctttaccc attcgtcatg ctccattaac gataaaaccg cccggaggcg gtttcacata
     3961 aaacattttg catcagcgac caatcaccac aacctgacca ccatcccctt cgtctgccgt
     4021 gctgatctcc tgagaaacca cgcgtgaccc cacgcgcatt tccccgtaca gaacaggcag
     4081 aacattgccc tgggcaacca tgttatccag tgaggagaaa taggtgttct gctt
//
LOCUS       DBANUU010000254         4133 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000254 DBANUU010000000
VERSION     DBANUU010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.259"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..428)
                     /locus_tag="ACIE68_004650"
     CDS             complement(<1..428)
                     /locus_tag="ACIE68_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFX5910824.1"
                     /translation="MRKLCAVILSAVVWLVAAGTPASAAEHQSTLSGGYLQSHTDMPG
                     NDDLKGINVKYRYEFTDTLGMVTSFSYAEDKNRQLTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRISTFSGDYLRVTDNKGKTH"
     gene            complement(498..3911)
                     /locus_tag="ACIE68_004651"
     CDS             complement(498..3911)
                     /locus_tag="ACIE68_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFX5910825.1"
                     /translation="MSKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     AITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDSQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRITDIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGT
                     LNNVTINQNCTIKGMLEATQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTISDDH
                     NFDRQIIIPPIIFNGIAYSDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGS
                     YSAVIDMPSGRGSVTLEFKVFHKGNQWAGNITDCTVIVTKKAASGISIR"
     gene            complement(3972..>4133)
                     /locus_tag="ACIE68_004652"
     CDS             complement(3972..>4133)
                     /locus_tag="ACIE68_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFX5910826.1"
                     /translation="KQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGD
                     GGQVVVIGR"
BASE COUNT          846 a         1275 c         1021 g          991 t
ORIGIN      
        1 tcgtgcgttt tccccttgtt gtcagttacg cggagataat ccccggagaa agtcgaaata
       61 cggctgtaag ccacacccgc catcgcatac gcgctgaacc attcattcac gcgcacagac
      121 ggccccgcca tcacgctgaa ccagcggtta cgcacggaat cttcatgcca gcgggtatcg
      181 ctgtaatgag tcagctggcg attcttgtct tctgcatagc tgaatgacgt caccatcccc
      241 agcgtgtccg taaactcata acggtatttc acgttaatgc ccttcaggtc atcgttgccg
      301 ggcatatcag tatgggactg aagatacccg ccgcttagtg tggactgatg ctctgctgca
      361 ctcgctggcg taccagcggc gaccagccag actactgcag acagaataac agcacataat
      421 ttacgcataa ttacctctcg cttttctgca ataaaaaagg caccatttct ggtgccctta
      481 tatgggttat aacaatttca acgaatactg atgccggaag cggctttttt ggtcacaatc
      541 accgtacagt cggtgatatt acctgcccac tgattgcctt tatggaaaac cttaaactcc
      601 agagtgacgc ttcccctgcc actcggcata tcaataaccg cactgtagct accgggaatg
      661 gcccctttag tttctctgga tgcgattaat acaccgtttt tgcgaacttc aaaaccataa
      721 cccgtgtatc ttgtacctcc cgggttatta ccacttcccg gatcgctata cgctattccg
      781 ttaaagataa tgggcggaat aatgatttga cggtcaaagt tatgatcatc gctgatggtg
      841 actgtaaccg tcccgtttgg tgtttccgtg ttaccccacg taccagcctg tttcggaaat
      901 gatttggata cagctttaac gaagtcacct ctgacctgag tcgcctccag catgccctta
      961 atcgtacagt tctggttaat cgtgacattg ttgagcgttc ctgagttcgc attcacacta
     1021 ccactgatat ccgcattttt agcggtcagc tttccgtctg atgtcaggga aaataccgga
     1081 ggactgccac cgctggtaat ggtgggggct gtcaggcgct tcaggaacac gtcgttcatg
     1141 aatatctggt tgccctgcgc cacaaacatc ggcgtttcat tcccgtttgc cgggtcaata
     1201 aacgcgatac ggttagcggc aaccagaaac tggctcagct tgccttcctc cgtgtcctcc
     1261 atgctgaggc caatacccgc gacataatgt ttgccgtctt tggtctgctc aattttgacg
     1321 ccccacatgg cattccattt atcgttagcg tccttccact ctttcgaaaa ctcatccagt
     1381 ctgctggcgt tatcctccgt cagatcgact ttttccagca gctccttgcc gagatgagat
     1441 tcggttatct tgcctttgaa aaaatccagg taaccttccg catcatcgct cgcccgaccg
     1501 acggcttcca cgaatgccga tttgccaacg gtgttcacac tgcggatata aaagtaataa
     1561 tcatggcccg gtttgatatt gatactggcg gctatccagt acagcgccgt accaagatag
     1621 cgggctgtgg tttcaacctg cctgatatcg gtaatccgct tttccgagaa ccagaactca
     1681 aactgtaccg tcgggtcata aacggcaaga tgcggcgtgg cggttatctg aaaatagccc
     1741 ggcgtcagct caatcctcga cggtgctgcc ggtgcggcaa tccggaacga taccgacgcc
     1801 ggatcgccct gctgccccca cgcatttacc gcccggactg tcagcctgta gttccccagc
     1861 gccagttgcg tgaagcggta tgtggtttcc gtcgtccggg ccgtgctgac cagccgctca
     1921 ctgccgtcat ccgctgttac ggtcagacgg agcaggaagc tcacgccctt caccaccttc
     1981 ggcgtgtccc agcgggccag cacctgatat tccccgctgt ctgcggtgac ttcggcggtc
     2041 aggtgctgca ccgctggcgg cgtgacacca ttcaccgtgc cgctctggtc gccgtcaaag
     2101 tgcgccccgt tatccacgat ggcttctttt tccggtacat gctgcacggc ggtgatggca
     2161 tacgtgccgt cgtcgttctc acggatactc acgcagcgga acaggcgctg gcgcagcgtc
     2221 ggcagcttca gcccccacac gctgtattca gcaacgccgt caggaacccg gctcactttc
     2281 accttcacgc cgtcggtgac ggactgaacc tccacgctga ccggattgcc acttccgtca
     2341 accaggctta tcagcgtggt accggaggat ggcagcgtga tttcacggtc gagcgtcagc
     2401 gtccgggtct gactgtttac cgccagcacg cgcccgccgg tgcggatacc ggcatagtca
     2461 tcatcacaga tttcaatgac atcgcccggc acatggcgaa gcccttcggc acccacgctg
     2521 aagtccaccg tctgcgtttc cagcagctcc gttttaatca gccacagccc ggcgcggtgt
     2581 gcctgccccc ggctggtaca gccaaaggca tccatcttcg tgacgttacg accgtaacgg
     2641 gcaatggcct gcgagtcctc cacaagctct gttgccgtct cccagccgtt atccgggtca
     2701 atccagttca cctcaacggc attatggcgg tctttcaggg cgctgaagct gtagcggaac
     2761 ggcgcgccat catccggcat caccacatta ctgcggttat aggtccacac cttatccgac
     2821 ggtcggtcct gcacgaacgt cagcgtctgc ccgttccata ccggcataca gcgcatcgcc
     2881 gagcagaaat cactgagcac atcccacgcc ttacgttgtg tggtcaggta agcgttacag
     2941 gtgatgcgcg gctccgtacc gccaaagccg tccggcaccg actggtcgca attctggccg
     3001 atgacataca gcgcccattt atccacatct gccgcaccaa gacgtttccc catgccgtag
     3061 cgcgggtggg tcagcatatc ccacagacac caggccatgt tgttgctgta tgccggtttt
     3121 aacgttccgt cccagatacc gctgtattgc cgcgtctgcg ggttataatt cgacggcacc
     3181 tgcagaatgc gcccgcgaag atgataatta cggctcacct gctggctgcc gaactgctcc
     3241 gaatccacct gcacgccgac cagtgccgtg ttcgggtagc actgtttcac atcgatgatt
     3301 tcggtgtatg acgaccagag cgttttgttc tgcagctggt ctgtggtgct gtccggcgtc
     3361 attctgcgca tccggatatt aaacgggcgc ggcggcaggt tatccaccac caccgaggcc
     3421 agatactgcg aggtggtttt gcccttaatg gtgatatctt tttccgtcac ccagccacca
     3481 ttacgctgta tctgaaccag caggcggact tccgacggat tccggtcccc ctttgaggtg
     3541 gtttccacca gtgcctgcac accgaaggta aagcgcagtc ggtcgatgtt tgccgacgtg
     3601 atggcgcggg tgatcggcgt gtcgtatttc acttccgtac ccaacaccgt ctcggagccg
     3661 gaggattcaa acccctccgg cggtgtctgc tcctgctcac cggcacggaa caccaccgtg
     3721 acaccggaga tgttggtatt cccctcagtg tccagcaccg gcgtactgtt cagcagcacg
     3781 ctttttaatc catccaccgg accttcaacc ggcccttcgc tgatggcatc gatcacactc
     3841 agcaactgcg tggacttcag gttgtccttc gcttcgcgcg gggtatgccc cttactgctt
     3901 cctttactca ttcctcacgc tccataaatg acaaaaccgc ccgcaggcgg tttcacataa
     3961 aacattttgc atcagcgacc aatcaccaca acctgaccac cgtccccttc gtctgccgtg
     4021 ctgatctcct gagaaaccac gcgtgacccc acgcgcattt caccgtacag aaccggcaga
     4081 acattgccct gggcaaccat gttatccagt gaggagaaat aggtgttctg ttt
//
LOCUS       DBANUU010000255         4122 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000255 DBANUU010000000
VERSION     DBANUU010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.260"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            102..842
                     /locus_tag="ACIE68_004653"
     CDS             102..842
                     /locus_tag="ACIE68_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910827.1"
                     /translation="MSQIFFDTIDNDQYDFMTEWNTAVMDKWVAENIGLSRCKDEAEL
                     FDTKWFDYRDMHPLMATCLFTEAYKRQYSNIMLTHGREHFETAPFTTGLKRLPYQELS
                     TANKTSLWKARQFADRYCCSYDYFISTVLSAAARRLWDKLPRPQHLWQPELIEIFEEK
                     LARRATTRLDDSLVSFKHIGDMQFNPIQESYFEWILERLRTIPRSKRIRAIFSAIWLM
                     EIVPERLISAHFPEELEEARRFIDPLSN"
     gene            886..2226
                     /locus_tag="ACIE68_004654"
     CDS             886..2226
                     /locus_tag="ACIE68_004654"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFX5910828.1"
                     /translation="MTELCHTGRGLSEEFDEDFQNRLTAYFCRDHEFLTRAGDLVVPS
                     QFANAANAILVNMVSGYYRMYKSAPSSSAILDMLKRAKRDKTIREELFADVVAAFKRI
                     LAEKLSDTSYMVDQVSTFAKSVAFDDALIKAAELKEKGDFQGAMAIMAKVQQIGSNEA
                     TGIYDYYTSASERLKAREYEASEEHVPNSITTGLPLLDRLLYQKGWAKREMVLFMGFA
                     KSGKSTAMGEFSINATLAGYNVLYLSLEVHTNILSDRFDARLSETEMSKLVERRDEVH
                     RKLAELGATKGIGSLWVVERPSGSMSPADLDRMLNSMKANGMVPDMLVVDYADLMRAS
                     YDLRDDRANIRSIYTDLRALYDKHNVAGITASQTNREGGASEVATMMHAADNIEKVRI
                     ADLVITINKTEEEEAKGEARLYFAGSRNQQGGISIRVKQNLEQMRFIERILDVT"
     gene            2374..3489
                     /locus_tag="ACIE68_004655"
     CDS             2374..3489
                     /locus_tag="ACIE68_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFX5910829.1"
                     /translation="MSELKELIAELDFEQWLDTEGIVYRRGGVSARGREVNIKECPVC
                     GSSNWKVYFNLTSGVGKCFAGDHPEEIQFNKLVFLKHYSGKSRRQFEEYVQNALLSQG
                     WAPKKEELVLASTVELEGPVALPRHYELPIDGRLPDYLVERNISPEMAKYFDLRYCVE
                     GKHAYVDPYTDQVKGQIFDMRILIPVYDLDGVMKTFQGRDITGTAERRYLFPMQLPAS
                     GKFLYNGHNAVGKQTVVVCEGAFDVMGVKRAIFDEETLRDYVEPIGTFGMHLSGNTTQ
                     DAEDQLGAFLTLKARGLRNVIMMWDSEKQAIRNTMAAARRLSSIGLNVKIACLGEEGL
                     DPGDATPGQIIKAYYCAKPYSRQLELLSKVKGIAALV"
     gene            complement(3481..>4122)
                     /locus_tag="ACIE68_004656"
     CDS             complement(3481..>4122)
                     /locus_tag="ACIE68_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFX5910830.1"
                     /translation="KAFTYYKKAADQGSSEAMIGLGILYDDGLGVKRNDAEAVKWYKK
                     AAELGNADAIINLGIMYENGEGVKKDYKKAADLYQTACDKGEKRGCDYIAELKESGKY
                     RAPASKAKTKSATQRLIAKSIDKGVNATFTWQGDDATFTANDGKVDCTFLKDFSEKGG
                     NLATSFVCTDNVQIILKQFRDTKNAYLAVMTDNFNTEVKSFSVNVYVTNTGSN"
BASE COUNT         1114 a          946 c          988 g         1074 t
ORIGIN      
        1 caccagaaca actcccttcc gtttccactt ccaaaaactg ccaccaatcg ctatcatcag
       61 ctcattgtga taagtaagta actacctacc aggtgagcca catgagccaa atctttttcg
      121 acaccatcga caacgaccag tacgacttca tgacagagtg gaataccgct gttatggata
      181 agtgggttgc tgaaaacatt ggtttgtcgc gctgcaaaga cgaggctgaa ctcttcgata
      241 cgaagtggtt tgattaccgc gacatgcatc ctcttatggc cacctgtctg tttacggagg
      301 catacaaacg tcagtactca aatatcatgc tgacgcacgg tcgcgaacac tttgaaacag
      361 ctccgttcac caccgggtta aaacgcctgc cttatcagga gttgtcgact gccaataaaa
      421 cgtcgctatg gaaagcacgc cagtttgctg accgctattg ctgctcatac gactacttta
      481 tctccaccgt tctttccgca gctgcacgac gtctgtggga caagctgcct cgcccacagc
      541 atttgtggca gccagaactg attgagatat ttgaagagaa attagccaga cgcgcaacaa
      601 cccgtctgga tgactctctg gttagcttta agcatatagg agacatgcag ttcaacccga
      661 tccaggaaag ctattttgag tggattctgg agcgtttacg caccatcccc cgcagcaagc
      721 gcatacgcgc aattttctcc gctatctggc taatggaaat cgttccagag cgccttattt
      781 ccgcccactt tccagaagaa ctggaagaag cacggcggtt tattgatccc ctatctaatt
      841 aactaatact agaaaacaat ttgtttaaaa aacaaaggaa agcacatgac cgaactttgc
      901 catacaggac gcgggctgtc cgaagagttt gatgaagatt tccagaacag actgacggca
      961 tatttttgtc gtgatcacga gtttcttact cgtgcgggag atctggttgt gcctagccaa
     1021 tttgccaatg cggccaatgc catattggtt aatatggttt cgggctatta ccgtatgtac
     1081 aagagcgcgc cctcttcatc tgcaattctg gatatgctta agcgtgcgaa acgcgataag
     1141 actatccgtg aggaactatt cgccgatgtt gttgctgcgt ttaagcgcat ccttgcagaa
     1201 aaattgtccg atacctcgta catggttgac caggtatcaa ccttcgcaaa aagcgtagcg
     1261 tttgatgatg ctctgattaa ggctgctgaa ctgaaagaga aaggcgactt tcagggggcg
     1321 atggcaatca tggccaaggt tcagcagatt ggatcgaacg aagcgaccgg aatctatgac
     1381 tactacacct ccgcaagtga gcgattgaaa gcgcgtgaat atgaggcttc agaggagcat
     1441 gtaccaaaca gtattacaac aggactccct cttctcgata ggttgctgta ccaaaaaggc
     1501 tgggcaaagc gcgaaatggt gcttttcatg gggttcgcta aatccggtaa atcgaccgca
     1561 atgggtgagt tttccataaa cgcaacgctt gctggctaca atgttctgta tctctcgctg
     1621 gaggttcaca ccaacatttt gtccgaccgc tttgatgcaa gattgtcgga gacggaaatg
     1681 tccaagctgg tggaacggcg cgatgaagtt catcgtaagt tggcagagtt gggagccact
     1741 aaggggattg gtagtttgtg ggttgttgag cgtccgtcag gaagtatgtc accggcagat
     1801 ctggaccgta tgcttaacag catgaaagcc aatggcatgg tgcctgacat gcttgttgtc
     1861 gactacgcag atttgatgcg tgccagttat gaccttcgtg atgatcgcgc caacattcgt
     1921 agtatctaca ccgatttacg tgctctttat gacaaacaca acgttgctgg tatcacagca
     1981 tcgcaaacaa accgtgaagg tggcgcgtca gaagttgcca caatgatgca cgctgccgac
     2041 aacatcgaaa aagtacgtat tgctgacctg gtaataacga tcaacaaaac cgaagaagaa
     2101 gaagcgaaag gagaggctcg tctctacttt gctggttcgc gtaaccagca gggagggatc
     2161 agcattcgcg ttaaacaaaa cctcgaacaa atgcgcttca ttgagcgaat cttagacgtt
     2221 acctaaaaaa taagcgtgga gaacacctcc acgcttgatt cattggtgaa acaacttttc
     2281 ttttgccaaa ccacaaaaga aaaacacatg agcctttatg ttatatcaac atttaggttg
     2341 gtcacaatat tgcctgttaa aagtggaatt atcgtgagcg agctgaaaga gctaattgcc
     2401 gaattagatt ttgaacaatg gttggatact gaaggtatcg tttatcgacg tggaggcgtg
     2461 agtgctcgcg gtcgtgaagt gaatatcaaa gagtgtccgg tatgcggcag ctccaactgg
     2521 aaggtatatt tcaatctgac cagtggcgtc ggtaaatgct tcgctggtga tcatcccgaa
     2581 gagattcagt tcaataagct ggtcttcctc aagcactaca gcggcaaatc acgacgacag
     2641 ttcgaggaat atgtgcagaa cgcccttctt tcccaggggt gggcaccaaa gaaagaagag
     2701 ctagtgcttg caagcacagt cgagttagag gggccagttg cactccctcg tcattacgag
     2761 cttcctatag atggccgtct tccagactat ctggttgaac gaaacatatc acctgaaatg
     2821 gcaaagtatt ttgacctacg atactgcgtc gaaggcaagc acgcttatgt agatccgtat
     2881 acagatcagg taaaagggca gatattcgat atgcgaatac tgataccggt ttacgatctg
     2941 gatggggtaa tgaagacatt tcagggacga gacattaccg gtacagcaga acgccgctac
     3001 ctctttccta tgcagcttcc agcttcaggt aagtttctct acaacggcca taatgcagtc
     3061 ggcaaacaga ctgtagttgt ctgtgagggg gcgttcgatg ttatgggggt taaacgagct
     3121 atttttgacg aagaaacatt acgtgattac gtggaaccaa taggaacgtt cgggatgcat
     3181 ctatctggta acaccactca ggatgcagaa gatcagttag gcgcgttcct gacgctcaag
     3241 gcgcgtgggt tacgtaatgt gatcatgatg tgggatagtg aaaagcaagc tatacgcaac
     3301 actatggccg cagccaggcg actgagcagt attggtctta atgtgaaaat tgcatgtttg
     3361 ggcgaggaag gactcgaccc cggcgatgcg accccggggc agattatcaa agcctactat
     3421 tgcgcaaaac cttactcacg acagcttgaa ctcttaagca aggtgaaagg cattgcagca
     3481 ctagtttgaa ccagtattag tcacatacac attgaccgaa aatgatttaa cttctgtatt
     3541 gaagttgtca gtcattaccg cgaggtaggc attcttggta tctctgaatt gcttcaggat
     3601 gatttgaacg ttatcggtgc aaacaaacga ggtagccaga ttgcctcctt tctctgaaaa
     3661 atccttcagg aaggtgcagt caactttgcc atcattagcg gtgaaggtag catcatcacc
     3721 ctgccaggta aatgtagcgt taacgccctt atcaatggat ttagcaataa gtcgctgggt
     3781 ggcggatttt gttttggctt tggaagccgg cgctcgatat ttgccgcttt ctttcagttc
     3841 tgcaatgtaa tcgcagcctc ttttttctcc tttatcgcaa gcagtctgat aaaggtcggc
     3901 tgctttttta tagtcctttt ttacaccttc cccattctca tacataatgc caagattgat
     3961 tatggcgtcg gcgtttccaa gttccgctgc ctttttatac cacttaactg cttctgcatc
     4021 attgcgttta acgcctaacc catcatcgta tagtattcct aatcctatca tagcttctga
     4081 actgccttgg tctgccgcct ttttgtaata agtaaaagcc tt
//
LOCUS       DBANUU010000256         4111 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000256 DBANUU010000000
VERSION     DBANUU010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.261"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..134)
                     /locus_tag="ACIE68_004657"
     CDS             complement(<1..134)
                     /locus_tag="ACIE68_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFX5910831.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     R"
     gene            complement(249..324)
                     /locus_tag="ACIE68_004658"
     tRNA            complement(249..324)
                     /locus_tag="ACIE68_004658"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(289..291),aa:Thr,seq:ggt)
     gene            complement(331..405)
                     /locus_tag="ACIE68_004659"
     tRNA            complement(331..405)
                     /locus_tag="ACIE68_004659"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Gly,seq:tcc)
     gene            complement(522..606)
                     /locus_tag="ACIE68_004660"
     tRNA            complement(522..606)
                     /locus_tag="ACIE68_004660"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(570..572),aa:Tyr,seq:gta)
     gene            complement(615..690)
                     /locus_tag="ACIE68_004661"
     tRNA            complement(615..690)
                     /locus_tag="ACIE68_004661"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(655..657),aa:Thr,seq:tgt)
     gene            1052..2002
                     /gene="coaA"
                     /locus_tag="ACIE68_004662"
     CDS             1052..2002
                     /gene="coaA"
                     /locus_tag="ACIE68_004662"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFX5910832.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2031..2996)
                     /gene="birA"
                     /locus_tag="ACIE68_004663"
     CDS             complement(2031..2996)
                     /gene="birA"
                     /locus_tag="ACIE68_004663"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFX5910833.1"
                     /translation="MKDYTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEKSV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(2993..4021)
                     /gene="murB"
                     /locus_tag="ACIE68_004664"
     CDS             complement(2993..4021)
                     /gene="murB"
                     /locus_tag="ACIE68_004664"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFX5910834.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAEALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1066 a         1021 c          913 g         1111 t
ORIGIN      
        1 cgagcagcac cgccgtaggt tttagccagt acggtagtga ttgcagcggt cagcgttgtt
       61 ttaccatggt caacgtggcc gatagtaccg acgttaacgt gcggttttgt acgttcaaac
      121 ttttctttag acatcgattg tccctctaag acacggataa atcggtgata tcaccacatc
      181 aaccaggcaa catgcccgac ttgttgaatg caataaacag aaggaaaaaa cagggaggag
      241 aaaagaagtg gtgctgatag gcagattcga actgccgacc tcacccttac caagggtgcg
      301 ctctaccaac tgagctatat cagcacatct tggagcgggc agcgggaatc gaacccgcat
      361 catcagcttg gaaggctgag gtaatagcca ttatacgatg cccgcatcct ggaactcggc
      421 tacctgattt tcattctgca ctgaatatcg agagaagctc tctttattcg agccggtaag
      481 cgaacttatc gtctcgggct acgccatcgc gtggccgaaa ttggtggtgg gggaaggatt
      541 cgaaccttcg aagtctgtga cggcagattt acagtctgct ccctttggcc gctcgggaac
      601 cccaccggac ttgatggtgc cgactaccgg aatcgaactg gtgacctact gattacaagt
      661 cagttgctct acctactgag ctaagtcggc atcaagtagc gcgcattcta tggagacatg
      721 cgagttcatg caactaaaaa attgcataat ttgttttatt ggtcacattt tatgcgacac
      781 gatgaagaaa cagccgaacg gagggcagat tagcacactt tttcaacatc attgtgctca
      841 acaatgcgct cctgctaaac cataattctt tttatcagat ggaatatctg tcacatagct
      901 tttcaacgat agcttcctgg cagagatttt ttcttattat tcctccccat ctggtgttac
      961 cctcctgccc attaacccat tcaacagaac tgtgacgcgc catggcaaat atcgctttgc
     1021 cgatagagct atgaccgcca gaaacatgct tatgagtata aaagagcaaa cgttaatgac
     1081 gccttaccta cagtttgacc gcaaccagtg ggcagctctg cgtgattccg tacctatgac
     1141 gttatcggaa gatgagatcg cccgtctcaa aggtattaat gaagatctct cgttagaaga
     1201 agttgccgag atctatttac ctttgtcacg tttgctgaac ttctatataa gctcgaatct
     1261 gcgccgtcag gcagttctgg aacagtttct tggtactaac gggcaacgca ttccttacat
     1321 tatcagtatt gctggcagtg tcgcggtggg gaaaagtaca accgcccgtg tattgcaggc
     1381 gctattaagc cgttggccgg aacatcgtcg tgttgaactg atcactacag atggcttcct
     1441 tcaccctaat caggttctga aagaacgtgg tctgatgaag aagaaaggct tcccggaatc
     1501 gtatgatatg catcgcctgg tgaagtttgt ttccgatctc aaatccggcg tgccaaacgt
     1561 tacagcccct gtttactcgc atcttattta tgatgtgatc ccggatggag ataaaacggt
     1621 tgttcagcct gatattttaa ttcttgaagg gttaaatgtc ttacagagcg ggatggatta
     1681 tccacacgat ccacatcatg tatttgtttc tgattttgtc gatttttcga tatatgttga
     1741 tgcaccggaa gacttacttc agacgtggta tatcaaccgt tttctgaaat tccgcgaagg
     1801 ggcttttacc gacccggatt cctattttca taactacgcg aaattaacta aagaagaagc
     1861 gattaagact gccatgacat tgtggaaaga gatcaactgg ctgaacttaa agcaaaatat
     1921 tctacctact cgtgagcgcg ccagtttaat cctgacgaaa agtgctaatc atgcggtaga
     1981 agaggtcaga ctacgcaaat aattttcagg ggagctaatg ctcccctttc ttatttttct
     2041 gcactacgca gggatatttc accgcccatc cagggtttta ttattccatc ctgctcaagt
     2101 aataaagccc cctgtttgtc tattccgcgt gaaatgccaa atatttcttt atcaccaatg
     2161 ataagtttca ctgggcgatt aataaaatta tccagctttt cccagcgcga cagataaggt
     2221 gccaatcctt cttgttcgaa gagttccaac gcagcacgta attcacgtat tagcatggcc
     2281 gccaacgtat tacgatcgag attgatcccc gcttcctgca gcgtgatcca cccctgatta
     2341 acgacactct tttcaacacg gcgcattgcc atgttgatcc cggctccaat gactatttgc
     2401 gccgcatcgc cagttttgcc agtcagctcc acaagaatgc ctgccagctt gcgatcctgc
     2461 agatagaggt cattaggcca tttgacacga actttatctg ctcccagctt gcgtaatact
     2521 tccgccatca cgataccgat aaccagactt aaaccaatcg ccgccgccgg gccttgttcc
     2581 agacgccaga acatcgacaa atataagttt gcgccaaaag gcgaaaacca tttccgcccc
     2641 cggcgaccac ggccagcctg ctggtattct gcaacacagg catcgcccga tttaagctct
     2701 ccgatacgat caagaaggta ctgattcgtg gagtcaataa ctggtagcac ggctacacta
     2761 ccgccatcca gctgacccaa tatctgttca gcattaagta actggatggg ctcaggcagg
     2821 ctgtatcctt tacccggaac ggtaaagaca tcaacgcccc agtcacgcag tgtctgaatg
     2881 tgtttattaa tagccgcccg gctcattccc agcgtttcac ccaactgctc gccagagtga
     2941 aattcaccgt tcgctaacag ggcaatcaat ttcagtggca cggtgtaatc cttcatgaaa
     3001 ttgtctccac tgcgctcact tcacctgatg caccaataaa gcggacttca ggctcaagcc
     3061 agacattaaa tttttcaccc actttttgtc ttacatggtg tgccagttgc accacatctt
     3121 cgctttttgc attgtcttca ttaatgagaa ctaacgcctg ttgacggtgc accgcagccc
     3181 cacccatttg catccctttt agctggcact gatcaataag ccaacctgct gccagtttta
     3241 ctgaaccatc cgcctggggg taatttggtg ctgttggaaa ttgtgacagt aatgcttcag
     3301 ccgtttcggc agatacaaca gggtttttga agaaactacc ggcattgcca ttcacttttg
     3361 gatcagggag tttggtggtg cgcatatgac acaccgcatt aaatacttgc tgtggcgtta
     3421 ctgttgtagg atccagacga gttaagtcac catacgttag tacaggttgc cactcttttg
     3481 gcagacgcag acctacggct acaatggcga agcgatcctg gtattcatgt ttaaaaatac
     3541 tgtcgcgata gccaaaacgg cactctttgg cagttaagcg cacttgcttg cctgtcgcca
     3601 gttcaacaca atcaacataa gcgcaaactc gctgtaattc tacgccataa gcaccaatat
     3661 tctggatagg tgatgagccg acacaaccag gaattaatgc cagattttcc agaccaggca
     3721 taccttcctg caaagtgtat tttaccaggc gatgccagtt ttctccggct cctacatgta
     3781 aataccacgc atcaggttca tcatgaattt cgataccttt gatccggttg atgatcaccg
     3841 tgccgcgata gtcttccaga aaaagtacat tacttccttc acccagaata agaacgggtt
     3901 gtccttctgc ggttgcatgc tgccaggcat tgagtaattg ttgttcgtct tcggcacata
     3961 caatgtgctg agcattatga tcaatgccaa atgtgttcca gggttttaag gagtggttca
     4021 tagctgcttt cctgatgcaa aaacgaggct agtttaccgt atctgtgggg ggatggcttg
     4081 tagatatgac gacaggaaga gtttgtagaa a
//
LOCUS       DBANUU010000257         4088 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000257 DBANUU010000000
VERSION     DBANUU010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.262"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..413
                     /locus_tag="ACIE68_004665"
     CDS             <1..413
                     /locus_tag="ACIE68_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910835.1"
                     /translation="PFNYEMDIATDSAGATERKNIDLVETFNYLIGLHVKSIESDIAR
                     GYVRIEGTLPTGERTLILWRDCDKIGYEELNKYANRFDLYAKENTFDVIYINGDHNLP
                     TAFTVDEEDGEIVRSLKIRQIEPEFLNLMFAEEV"
     gene            416..3796
                     /locus_tag="ACIE68_004666"
     CDS             416..3796
                     /locus_tag="ACIE68_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFX5910836.1"
                     /translation="MAKKPTTGKAVKKETNSKLSFHKQLVLNRFMFHFFKDGTLQGLK
                     NRLGEDRFEGIHEDGQSLFFHELSNYLFEVDLIDLDELRRYDLNIVQHWQQITEHRNR
                     KEDTVLNMKYFQYLSLLFTEIYLDWYFNRKQQLLDGLNNELAAYNAENDKTAKDRANQ
                     FKSYVLDDLNKLAYWNATGSGKTLLLHVNILQYLHYFQNGNSHHYPDKIILLTPDERL
                     SKQHLDELENSGFTQYQLFDKSKSAPFKGTIEVIDINKLDDKMGDKTVAVEAFEGNNL
                     VLIDEGHKGTGSAAGAWMRRRDKLTRDGFAFEYSATFGQAVAGGNNVEAVETEIQKKK
                     AKLLFETTALGKLSEEELAQIALTSEEQRDARIQATREVYGKSILFDYSYKFFYEDGY
                     GKESLILNLNPQDDKKDERRYEYFTACLLSFYQQQYLFNKNKEKLNEFNIEKPLWVFV
                     GNSVSGEDSDIHAVLRFLAWFLNHETQAKAWINDLIKNKARILDTKERNIFENRFTAL
                     MNAPEDIYADILSRLFNTTHSGQRLRVLNLIGSKGELALQVGEAEPFGLINIGDAPSL
                     YKMCEEDTTFDYQRDDFAKSLFHTLNDKDSRLHILIGSRKFTEGWSSWRVSTMGLLNM
                     GRGEGSQIIQLFGRGVRLKGRNYSLKRSTPGERPKGLHLEKLETLNVFGVRADYMATF
                     KQYLEDEGITPSDEILELNFETRPNMPATRLKTLKLKDGYKDNQRLGFKRVYFPELYE
                     VPADFQGKIKQPHIKLDLYPKLESIDTSRKGENTPVNVRNEAKLDYKIISAFDFDRLY
                     LAIQNYKLQRGWSNLRVDKQRLIDFCTGKTKIGNSWYTLLIPASELEIKQYADIAKQE
                     DIMLRLLTDYTDRFYNTLKNAYEGQFYEVTQVKEDDPGMIKMYHFEIEETDDGLAYAS
                     RLEQLQQLVIDGEIGKAKSWSASGMVAITFDKHLYYPLLSIEKNANIPLKMRPAALGA
                     SSEITFVHDLQSFVDSTKGQKIIGDKSLYLLRNADSKNRGLGFATAGNFYPDFLLWLV
                     DDKSGKQWLSLIDPKGIRNLNLDDPKFGLYKEIKNLEHKLSDDNLSLSAFIVSETCYV
                     DLVNVPDSKENLEARNVLFIEDTGPLYLEKLFSKMVSE"
     gene            complement(3843..>4088)
                     /locus_tag="ACIE68_004667"
     CDS             complement(3843..>4088)
                     /locus_tag="ACIE68_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910837.1"
                     /translation="IEGEKRLASERDATETVYNLFGQPTWKNLYLLDMFNLKELQGII
                     ECRKNGQSFDQERHREIIKMLEYAAKSFDLIIPAHWR"
BASE COUNT         1268 a          754 c          887 g         1179 t
ORIGIN      
        1 aaccatttaa ctacgaaatg gatattgcga cggattccgc cggtgccact gagcgtaaaa
       61 acatcgattt agttgaaact ttcaattatc tcattggctt acatgtgaaa tccatcgaat
      121 ctgatatcgc acgtggctat gtgcgtattg aaggaacgct gccaacaggc gagcgtaccc
      181 ttattctgtg gcgcgattgc gacaagatcg gttatgaaga attgaataaa tatgccaacc
      241 gatttgattt gtatgcaaaa gaaaacacgt tcgacgttat ctacatcaat ggcgatcata
      301 acttacctac cgcctttacc gttgatgaag aagacggcga gattgttcgt agcctgaaaa
      361 ttcgccagat cgaacctgaa ttcttgaacc tgatgtttgc ggaggaggtt taagaatggc
      421 taaaaaaccg accaccggta aagcagttaa aaaagagact aacagcaagc tgagctttca
      481 taaacaactg gttttaaacc gctttatgtt ccattttttc aaagatggca cattgcaagg
      541 tttgaagaat cggttaggcg aggatcgctt tgaaggcatc catgaagatg gccaaagctt
      601 gttcttccat gagctgagta actatttatt cgaggtcgat ctaatcgatc tcgatgaact
      661 gcgtcgttac gatctcaata tcgtgcagca ctggcagcag ataaccgagc accgtaatcg
      721 taaagaagac actgtgctga acatgaaata ttttcagtac ttgtcgcttc tgtttaccga
      781 gatctacctg gactggtact tcaatcgtaa acagcaatta ctcgatgggc taaataacga
      841 gctggcagca tacaatgctg aaaatgataa aacagcaaaa gaccgggcta atcagttcaa
      901 atcatatgtt ctggatgatt tgaataaatt ggcatactgg aacgctactg gttctggtaa
      961 gacactgcta ttacatgtca atattttgca atatttacat tatttccaga acggtaatag
     1021 ccatcattat ccggataaaa tcatcctgct tacgccggat gaacgcctgt cgaagcaaca
     1081 tttagatgaa ctggaaaact cagggtttac ccaataccag ttgttcgata agagtaaatc
     1141 agcgcccttc aaaggcacga ttgaagtcat tgatatcaat aaacttgatg ataaaatggg
     1201 cgacaaaact gttgccgttg aggcatttga aggcaacaat ctggtcctga ttgatgaagg
     1261 gcataaaggc acaggaagtg ctgctggtgc ctggatgcgc cgtcgtgaca agttaactcg
     1321 tgacggattt gcgtttgaat attcagcaac ttttggccag gcagtagccg gtggcaataa
     1381 tgtggaggca gttgaaacag aaattcagaa gaaaaaagca aagctgcttt ttgaaacaac
     1441 tgccttaggc aaattgagcg aagaagagtt agcacaaata gcattaacca gcgaagaaca
     1501 acgtgatgcg cgtattcagg cgacgcgaga agtctatggg aaatcgattc tgttcgatta
     1561 ctcctataaa ttcttctatg aggatggtta tggcaaagaa tcactgattc ttaaccttaa
     1621 tccgcaagat gataaaaaag atgagcgtcg gtatgagtat tttaccgcgt gtttgctcag
     1681 cttttatcag caacaatatt tattcaataa aaataaagaa aaattaaatg aatttaatat
     1741 cgaaaaaccg ctgtgggttt ttgtcggtaa ttctgtaagt ggtgaagatt cagacattca
     1801 cgcagtattg cgttttttag catggttttt aaaccacgaa acgcaggcaa aagcgtggat
     1861 taacgatctc attaaaaata aagcgcgaat cctcgatacc aaagagcgta acatttttga
     1921 aaatcgcttt actgcactaa tgaacgctcc ggaagatatc tacgcagata ttctttctcg
     1981 cttgtttaac accactcact ctggtcagcg actgcgagtt ctgaatttaa taggcagtaa
     2041 aggtgaactg gcattacagg ttggtgaggc cgaaccgttt ggccttatca atattggtga
     2101 tgcaccgtct ctttacaaaa tgtgtgaaga ggatacgaca tttgattatc agagagatga
     2161 ttttgcaaag tcgctctttc atacgttaaa tgacaaagac tcgcgactac atattttgat
     2221 cggctcgcga aaatttactg aaggctggag cagttggcgc gtctcaacga tgggattgct
     2281 caatatgggc cgtggagaag gttcacagat cattcagtta tttggccgtg gcgttcgcct
     2341 taaaggtcgc aattattctt tgaaacgcag tacacccggt gagcgtccga aagggttgca
     2401 tctggaaaaa ttagagacac tgaatgtatt cggtgtacgt gccgattaca tggcaacctt
     2461 taaacaatac ctggaagatg aagggattac gccaagtgac gagatccttg agctgaattt
     2521 tgaaactcgg ccaaatatgc cagcaaccag gcttaaaaca cttaagctca aagatggtta
     2581 taaagataat caaagactcg gctttaaacg tgtctatttt cccgaattgt atgaagttcc
     2641 tgctgatttt caggggaaaa ttaagcagcc gcatattaag ctggacctat acccgaaact
     2701 tgagtcgatt gataccagca gaaaagggga aaatactccg gttaacgtgc gtaatgaagc
     2761 gaagttagat tataaaatca ttagtgcgtt tgattttgat cggctttatc tcgccattca
     2821 gaattataaa ctacaacgcg gctggtcaaa tttacgtgtg gataaacaac gtttgatcga
     2881 cttttgtacc ggtaagacaa agatcggaaa tagttggtat acccttttga ttccagcttc
     2941 agaacttgag attaagcaat atgcagatat tgctaagcag gaagatatta tgcttcgtct
     3001 gctgactgat tatactgatc gcttttacaa cacattgaaa aatgcttatg aaggtcagtt
     3061 ttatgaagtc acacaggtga aagaagatga tcctggtatg atcaaaatgt accatttcga
     3121 gatcgaagaa actgatgatg ggttggcata tgccagtcgg ctggaacaat tacaacaatt
     3181 ggtcatcgac ggcgaaatcg gtaaagcaaa atcctggagt gcatccggga tggttgctat
     3241 tacctttgat aaacatctct attatccact tctttcgatt gaaaaaaatg ccaacatacc
     3301 gctaaaaatg cgaccggcag cactgggtgc atcaagtgag attacttttg tccatgatct
     3361 tcagtctttt gttgattcga caaaagggca gaaaattatt ggtgataaaa gtttatatct
     3421 gctaagaaat gcggattcta aaaatagagg cctgggcttt gctactgcgg gtaattttta
     3481 tccggatttc ttattgtggt tagtcgatga taaaagtggt aaacaatggt taagtcttat
     3541 tgatccaaaa ggcatccgca atcttaatct tgatgatcca aagttcggat tatataaaga
     3601 aatcaagaat cttgaacata aattatcgga tgataactta tcattaagtg catttattgt
     3661 ttctgaaact tgttatgttg acttagtaaa cgtacctgat tcaaaagaga atctggaagc
     3721 cagaaatgtg ctttttattg aggatactgg tccattgtat ctggagaaat tattcagtaa
     3781 gatggttagc gagtaaataa atatgattct gctccatatc tctgtgagat atggagcaga
     3841 tattatcgcc aatgtgcagg aataattaag tcaaacgatt ttgctgcata ttcaagcatt
     3901 tttattattt ccctgtgtcg ttcctggtcg aaagactgtc cattttttct acactctata
     3961 atcccttgta attcttttag gttaaacata tcaagaagat ataaattttt ccaggttggt
     4021 tgcccgaaga ggttatagac tgtttccgta gcatctcgtt cacttgctaa tcttttctct
     4081 ccttctat
//
LOCUS       DBANUU010000258         4081 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000258 DBANUU010000000
VERSION     DBANUU010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.263"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..204)
                     /locus_tag="ACIE68_004668"
     CDS             complement(<1..204)
                     /locus_tag="ACIE68_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFX5910838.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSP"
     gene            complement(218..2698)
                     /gene="yehB"
                     /locus_tag="ACIE68_004669"
     CDS             complement(218..2698)
                     /gene="yehB"
                     /locus_tag="ACIE68_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFX5910839.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRFGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLATWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(2714..3388)
                     /gene="yehC"
                     /locus_tag="ACIE68_004670"
     CDS             complement(2714..3388)
                     /gene="yehC"
                     /locus_tag="ACIE68_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFX5910840.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(3469..4011)
                     /gene="yehD"
                     /locus_tag="ACIE68_004671"
     CDS             complement(3469..4011)
                     /gene="yehD"
                     /locus_tag="ACIE68_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5910841.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT         1148 a          998 c          753 g         1182 t
ORIGIN      
        1 cgggctcata ggggtgatag tatttgtact cacgcatgac gaatcattaa aattatgctc
       61 atatgtcgcc atttgtgttt gagtaagtgc aaaatccccc tgaacaacca aattgtgctg
      121 aacagataac tcactataac atgccgcata gctcacaggc ataaccatca tcattactat
      181 aaataacatt atacgaatat tcataaccac ctgactatta ctggcaaata tagtttctac
      241 tttcatcaat ctctttaccg aaggtgattg tgcatgaaag tccttgttgc ttatcaattg
      301 ctacattaac cgatggcggt acttcattgg tgcgaataaa taactggctt ccctgaccga
      361 caacgccaat attatgacca tggatatcat tgacttcata accaaacatt aatggttgcc
      421 catccgctct taacgctttt ataaaccagg gcttgcgctg atcggtatca aaattaacca
      481 gtacaaccgc gccgcgataa ggggcggcaa ttttccggtt gccacgtaat tctgcttcgc
      541 tatcgctttg cgacacatcc agcatcaggt gattttcccg ataaggtgtc attccgtcgt
      601 ataccaccac tccattacgg tttgttgtgc gatatttttg cccattgaca taagcatctt
      661 taattcctgg cgcattcatc acagcaaacg tttcggaaag acggttcgcc agattaacgc
      721 cccccgacca ggcgacaatg ccccctgaaa cactggctcc agcctgtcga taagtactcg
      781 actgactata actgccattc actgtcgcaa ccggcgcgtt ccaggtcaaa ttcgccccag
      841 ctgtcgtttc atttccctga ttctgataac tcaggttgac cccatagtta aactgatccc
      901 ggcttccaac ggttcctgat aatcccgtat tatttgaggc aaacccctga tcatcaaacg
      961 tcgttgagtt agacatatat atttgccgac gaggcgtcgt aacgtcatca ccccaatcaa
     1021 agggaatcga tataaaaata ttaaaacgtt tctcttcatg atgattctcg tcatacgcct
     1081 ggcttgccgc gaggatatag cttatccgcc gccagttatt ggaataactc aactgataat
     1141 ccttactgct gccgctacgc ccccagtaat ctcgccataa cgtacttaag gacacagacc
     1201 cccagccttc tggcaatgac tggctcatat tggcagagaa gctattttta cggccaaaat
     1261 cgttctggta ataatcggca atgtcataga catcgttttc atcacggcga taattatctt
     1321 tattgtttgc ccaaacgtga tcattaaatg tccggtaatc acgcgatgaa taacgccagg
     1381 tcgccagacc aaaacgcgtc gacgtttggc tcacaaattt gttgtaggca atttgataac
     1441 tttgcccgtc aaacacatcg ccgttgtctt gtttactatg cgacttcgtg gcatcgacgg
     1501 aaatggcacc aaagcgtgta ttccagccag tccccaaagt aaacgcgtaa taattattcg
     1561 cgaccatcga gccaccatac agcgtcaata aattattaaa accatactga taacccgcct
     1621 ggacaaaatc actttgtttg ctcgcccctt caatatggct acgacccgcc gcaaaatcat
     1681 atttcgatac gccgggttgc agcatatttg gcactgctgc ataaggcacc agataggtgg
     1741 ttaccgagcc gtccgcctct ttcacgctga catcaagatc tgctccacca ccggccaact
     1801 gcaaatctgt aatcgcgaac gggccaggag gaacctcttt ctgataaacc acaaaaccgt
     1861 tctgttcaat agttaccagc gcattactct gagcaatccc ctgcacccgt ggcgtaaaat
     1921 tttgtttcga gttaggcaac atctgcatat cacgaaacaa ccgcacaccg ctgaagcgaa
     1981 cagaatcaaa aatatcgctt gatgtgtaca tatcacccac gcgaagcgtg ccgagaagtt
     2041 gggcaaatcc acgttccaga tacagggtat tgcttttcca cacccctgga ttgttatttg
     2101 ttttactgaa actggcatca gaatgcagtt gccaccccag taaatttaac ccgctgttaa
     2161 aacgtacata tgtactctta ctattacccg acgctttata gtcactgtaa tactgactca
     2221 cataataaga ggtataaaac gcattaatac cccgctccca gttttccggt ggaacatagc
     2281 cactttccag ttcttccacc caggcctgcg ggacactgaa atcgagacga aaaaccccga
     2341 tatcccaggt atagctccca ccctgaacaa gttgctcaaa tgttaaacat tgcttaccgc
     2401 tggcgaagtt atcgctatta atgcctaacc gcttgataac ttctattgat aaacatgttt
     2461 cttgcgggtt gtctttaaca ataatctcat atttcccgcg ccattgctta ttgacataaa
     2521 tatcgatgtc atactgcccc ggtaagggtt gattatcatc aagacgaata tttgcaacct
     2581 gctggtcttt cattccacct atcataaaat gggtatcaaa ggtttcttca gctgcataag
     2641 cttcaatgcc gagcaataac gctacgattg ctgatgcaag tggggtcatt ctcaacatag
     2701 gcagctcctg caattaaatt ttgtcactaa tatagttgcc gtggtcatcg ataatggtca
     2761 gataccagtt atttgcatta ttacttttga cattaacact ctgactttct aagggggcaa
     2821 tcataatagt ttcataattg actttaacat tattagcttt gacatccgaa atcgtcaccc
     2881 aattagctga gtcatttttt atcaccaatc cattgccact gcgatttacc agtaactttt
     2941 taaaggtcgc tttatttacc ggagcaatac ccgctggccg gtaaaacaac ttaattctgt
     3001 tttgcatcgc aaacttcagt gcatttttgc cttcttgctc tggactattt ggtggaatat
     3061 ccagaacatt cagataaaaa atgctttctt tattagtggg aagtttattc ggcataattt
     3121 tgatttttac ttgctgcccg gaatttgccc ctatttttgc cactggtggc gttaacatga
     3181 aaggaacctg aattttttct ggtggtaacg acgtatcgcc atcatcaatc cacgcctgca
     3241 gcagcgaaga acggtttccc tggttcatca actgcaccat aacttcttta ttttctgccg
     3301 ggtaaataat gcgcgtcccg tagataacaa ttccggcatg tgcaggaagg accatagaaa
     3361 aaatgagtaa agataataat cctttcatct taatgcctct taaattaaaa agccgccatg
     3421 gggatagcgg ccatgtttat tacctggaga aacttacaat tttatttatt aatcctgcgc
     3481 cacacgcata accagagaag ataacacatc acctgattgc actgagttat ctgtcagttt
     3541 caggtatttt gctttaaacg gaatagcata ttgaccttcc gctgttttat aactctcaat
     3601 gttaaaaggt gcagaaacat ccaggacatc gctgttatca cctaaataga gatagtaacc
     3661 cacggtatcg ttgctactgg ctatcgcctt atcgttaccg gttattttat cgttggtaat
     3721 caacgagagt tttacaccag caacttcttt cgggcagcta atttttaaca tgctgctggt
     3781 tacaccgact gcatcatcat aggtagaata tttagcttta ccttcaaaga cagtggttgg
     3841 tacagtcggc atattaatac ttactgaatc accacccgct tcgaatttac acggagattc
     3901 tgcaatattc cccttaatag ttaatgttcc ggtatcaacg tctgcagcaa atactccagc
     3961 gctcataaaa aaagaagaaa agacagcggc agcaataatt gaacgtttca taatatgttc
     4021 cttaataaaa tttatttttg gttgttaaga gtctgtatct cttggaaaca aatttctcac
     4081 a
//
LOCUS       DBANUU010000259         4054 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000259 DBANUU010000000
VERSION     DBANUU010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.264"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            53..1711
                     /gene="dpiB"
                     /locus_tag="ACIE68_004672"
     CDS             53..1711
                     /gene="dpiB"
                     /locus_tag="ACIE68_004672"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFX5910842.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1680..2360
                     /gene="dpiA"
                     /locus_tag="ACIE68_004673"
                     /gene_synonym="citB"
     CDS             1680..2360
                     /gene="dpiA"
                     /locus_tag="ACIE68_004673"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFX5910843.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2401..3786)
                     /gene="dcuC"
                     /locus_tag="ACIE68_004674"
     CDS             complement(2401..3786)
                     /gene="dcuC"
                     /locus_tag="ACIE68_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFX5910844.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1111 a          952 c         1045 g          946 t
ORIGIN      
        1 acattttaat tacatccgtc aaagaggctc gggacaaccc gcaaggaaaa caatgttgca
       61 gcttaacgag aataaacagt ttgcattttt ccaaagactg gcatttccgc tgcgtatctt
      121 tttgctgatt ctggtgttct caatatttgt cattgcagcc ctggcgcaat attttacggc
      181 cagttttgag gactatttaa cgcttcatgt acgcgacatg gcaatgaatc aggcgaaaat
      241 tattgcctcc aatgacagtg tcatctctgc ggtgaaaacg cgtgactaca aacggctggc
      301 gaccatcgct aacaaattac aaagagatac cgattttgat tatgtggtga ttggggaccg
      361 gcactcgatc cgcctttacc atcctaatcc ggagaaaatt ggttatccta tgcagttcac
      421 caaacagggc gcgctggaga aaggggagag ctacttcatt accgggaaag ggtcaatggg
      481 gatggcgatg cgcgccaaaa cgccaatctt tgatgacgat ggaaaagtca tcggcgtggt
      541 gtcgattggc tacctggtga gtaaaatcga tagctggcgg gctgagtttt tattaccgat
      601 ggcaggcgtg tttgtcgtgc tgttagggat tctgatgttg ctgtcgtggt tcctggcagc
      661 gcatatccgt cggcaaatga tgggcatgga gccaaagcaa atcgcacgcg tggtccgtca
      721 gcaagaggcg ctgtttagtt cggtttatga agggctgatt gcggtggatc cgcatggtta
      781 cattaccgcc atcaatcgta acgcaagaaa gatgctgggg ctgagctctc ccggacggca
      841 atggttgggt aaacccattg ctgaagtggt caggcccgcc gatttcttta ccgaacagat
      901 tgatgaaaaa cgtcaggatg tggtggctaa ctttaacggt ctgagcgtta ttgccaatcg
      961 ggaagctatt cgttcaggtg atgatttgct gggggccatt atcagctttc gtagtaaaga
     1021 cgaaatttcc accctcaatg cgcaactgac gcaaataaaa caatacgtcg agagccttcg
     1081 tacattgcga cacgagcatc tcaattggat gtcgacgctc aatggtctgt tgcagatgaa
     1141 agagtatgat cgcgtgctgg cgatggtgca gggggagtct caggcccagc aacagcttat
     1201 tgacagcctg cgtgaggcgt ttgccgatcg ccaggtggcg gggctgcttt ttggtaaagt
     1261 gcagcgcgcc cgggaactgg ggctaaaaat gatcattgtc cccggtagcc agctttcgca
     1321 actgccgcca ggactggata gcaccgagtt tgcagccatt gtgggcaatt tacttgataa
     1381 cgccttcgaa gccagcctgc gtagcgatga aggaaacaag atcgttgaat tattcctcag
     1441 cgatgaaggc gatgatgtgg tgattgaagt cgccgatcag ggctgcggcg ttccagagtc
     1501 tctacgagac aaaatatttg agcagggggt cagtacgcgt gctgacgagc ccggtgaaca
     1561 tggcattggg ttgtacttga ttgccagcta cgtaacgcgc tgcggtggtg ttatcactct
     1621 cgaagataat gatccctgcg gtaccttgtt ttcaatctat attccgaaag tgaaacctaa
     1681 tgacagctcc attaacccta ttgatcgttg aggacgaaac gccgctggca gagatgcatg
     1741 cggaatatat tcgtcacatt cccggattca gtcagatatt actggcggga aacctggcgc
     1801 aggcccgaat gatgatcgag cgttttaagc cggggctaat cttgctcgat aactatcttc
     1861 ctgacggcag agggattaat ttattgcatg aactggtgca ggcgcattat cccggcgacg
     1921 tggtgtttac cactgcagcc agcgatatgg aaacggtgtc tgaagccgta cgttgtggtg
     1981 tatttgatta tctcattaag cccattgcct atgaacggct ggggcaaacg ctaacccgtt
     2041 tccgccagcg taaacatatg ctggaaagta ttgatagcgc cagccagaag caaattgatg
     2101 agatgtttaa tgcctatgct cgcggtgaac ctaaagacga actgccgacc ggcattgatc
     2161 ccttgacgct aaacgcggtg cgaaaactgt ttaaagagcc tggtgtgcaa catacggcag
     2221 aaacggtggc gcaggcactg accatcagtc gcaccactgc caggcgttat cttgaatatt
     2281 gcgccagccg ccatctgatt attgctgaaa ttgttcacgg caaagttggc agaccacaac
     2341 gcatatacca cagtgggtga catgataaag ccggaggaaa cttgcctccg gcattactat
     2401 ttacttgcct gtgaccgctg ctgccgttcc tggcaccatc agctctgtag caacgataac
     2461 aatcactagc ccaacaagca ccggcaccga ggtgcgtttt acgacttcaa atggtgagat
     2521 tttcgccatc ccggcaaccg caacgactac gccagaaacg ggtgataggg tacgacccag
     2581 gtttgacgcc tgcagcatcg ggatagtcaa atacgccggg ttaatgccgg aagagtgcgc
     2641 cagtttcggg atcatctcaa caaacgcata aaacggcgca ttgcctgaac cggtcgtgac
     2701 tgccgccagc atggtcagaa tcaccaatac cagcatcagg atgatactcg ccgaaccaaa
     2761 cgaggtagcg atagaaatca gactttgaat aaagccgatg gtgctaagcc cctgagcgaa
     2821 taccccagcg gcaaccagca gcatcaccac gttagcaaac gcatctgcca tcccgcgata
     2881 agccacttcc agaccagaga aaactttctg ggtattaaag ctgcggagga actccagaat
     2941 ggaggcaatc agcatacaaa tcaccagaat agtgatgatg tgtaattgcg gaccccattt
     3001 accgtcaaaa atcagtacac cgatgatcgg cgtgaacggc aaaatggcat aaaacgcagg
     3061 agcagtggtg gtgatttcac tgacatctaa catttcatga gagatgtgct cttttttatc
     3121 cagataacgt tgccagaaga agtgggcgat cgccatgccg ataattgcag caattgagat
     3181 aggcagcgtc gttttgaagg cgaagtcaat cagcgacatt tcggaagctt gcgccgccag
     3241 caccacatcc cctgaagtcg gtgcgagaat aatcgccgcc ggggaggcac aaatggcagc
     3301 agctgcgcca cgactgatac caacgtttac catcaccgga aatagggttg ccatcagcaa
     3361 aacacccaga ccggttgcgg aagagacggc cagagacatc agacaggcga caaaataggc
     3421 ggcaatcatc agcaggtaag gggagttaat atactgcaat ggttttgacg ccagcttgac
     3481 caccatatca ttcgcgccga tatgggtcat gtaagcggca aatccacaca gcatcataat
     3541 catcatgccg aggtcgccgc cgcggctcat tagtaatatt ttaacgtatt caacgatatc
     3601 cgtggcgctg tagcctgttg aagcctggct ggacggtaac actttgtgcc ccataatggc
     3661 actgataatc agcaataaca ggccaccgac aaataacaca ccagtggcgg aatacccttt
     3721 aatgatgtag cgagctacac ccacaataac cacaacccca ataaggagct caatgaatgt
     3781 cagcataatt tttcctgtct ccaggcccca aagtaaataa taaaaaattc ctaaagctta
     3841 aggaaaaaat atgcccaata aattggcgat gaatgctgat taaaatcaag aaaaactgcc
     3901 attaagacat tgaagttgct gtttttatac atagataaca aaacgcatat ttagcggttg
     3961 gcaattatca cataataatg tgtgcaaatt gatggtggat tgatgcaaat ttgttaattg
     4021 caatggtgtt agctgcggct gtgcgctcaa aaaa
//
LOCUS       DBANUU010000260         3933 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000260 DBANUU010000000
VERSION     DBANUU010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.265"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..561
                     /gene="ykgH"
                     /locus_tag="ACIE68_004675"
     CDS             <1..561
                     /gene="ykgH"
                     /locus_tag="ACIE68_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFX5910845.1"
                     /translation="LLFLYINKDNIKVIYSLKINQTTPGILVSCDSNNNFACQTTMTE
                     DVIQRITTFFHTSPDVKNREIRLEWSGDKRALPTAEEEISRVQASIIKWYASEYHNGR
                     QVLDEIQTPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGF
                     FLSIVISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(804..1499)
                     /locus_tag="ACIE68_004676"
     CDS             complement(804..1499)
                     /locus_tag="ACIE68_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFX5910846.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVILFSAAERGRSLSLLP
                     EYSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1492..2919)
                     /locus_tag="ACIE68_004677"
     CDS             complement(1492..2919)
                     /locus_tag="ACIE68_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFX5910847.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFARTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2930..3649)
                     /locus_tag="ACIE68_004678"
     CDS             complement(2930..3649)
                     /locus_tag="ACIE68_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFX5910848.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPMYLADEPEWASRAA
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELFTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1033 a         1040 c          822 g         1038 t
ORIGIN      
        1 ctgttgtttc tgtatatcaa taaagacaat ataaaagtga tttacagcct aaaaataaac
       61 cagacaacgc caggtatact tgttagctgt gatagcaata ataattttgc ctgtcagact
      121 acaatgactg aagatgttat tcagcgaatt actacatttt ttcacaccag cccagatgtc
      181 aagaacagag aaataaggct ggaatggtca ggagataaga gagctttacc aactgctgaa
      241 gaggaaatat ctcgcgtgca ggcctctatt atcaaatggt atgcgtcaga atatcataat
      301 ggcaggcaag ttctcgatga gatacaaacg ccttcagcaa ttaacagtga gctttataca
      361 aaaatgatat acctgaccag gaactggtca ttgtatccga acggtgatgg ctgtgtaact
      421 ataagctcac cagaaataaa aaataaatac cctgctgcca tttgcctggc tctgggattt
      481 tttctaagca ttgtaatttc tgtaatgttt tgccttgtca aaaaaatggt agatgaatac
      541 caacaaaact ctgggcagta ataaacaaca ttctgataga gagtgcctgt ataaagctga
      601 agtgacaatg accgggaagg gaacccgttt tcaggaatgg atgcctgtct ttttcactgt
      661 aaatatcctg gcattggtaa taccaggata tttacagaag agcagcataa ccatgacgga
      721 acggggttaa gtcaggttgt cctaaacgct attttcaacc tcgtatgcct tcttcaggtt
      781 tatgtccaga cttcatatct ctctcaacaa tcctcaataa tcagatacac cgctttcacc
      841 gggccatgaa ctccgacgac tttgataagc tcaatatccg ccgttgaact ggggccgcta
      901 atgatgttaa tgcaggaagg cattcgttca ccggcctgcg ctttctgatg caatttttct
      961 gcgagttgcg ctacacgcgg caggatagtg cttttacgca ggataaaaag agaatattcc
     1021 gggagcaggc tcaatgaacg cccgcgctcg gcggcggaaa aaagaatcac gcctcccgat
     1081 tcggttaaac catattcagc atacacaaca cccactttag cctgctctgc ctgcgagata
     1141 ttctcggcac ctttcgccgg atcccaaaca acggcattgc attcctgctg caaacgttcg
     1201 ctaatcccca attcctccag cctcgtgtca ccgctaatca cgaccgactg atctcccagc
     1261 tctttacaca gacgtattgc agcttctgcc gccttcgcct cgctggtcag ctcacagcgc
     1321 gtcaacataa catcgctggc aaactgaata aacgcgtcac agcgctgctg ttggttaagt
     1381 tgggtaagcc gctcgttagc atagttgtta agcggcgcat cttctgcttg cggttcaagt
     1441 cgcagcgggc gacccagtgc ctgagcaacg ttattcaaaa attcgcctcg attatccatt
     1501 ctttttctcc tgcgcctgat gtttcttaaa ccaactacgg aaactctctc cgtcagcttc
     1561 aggaagatcg cgtgcttcca tccagtcgct aatcgcgcca aatttgagtg gtgttttgcc
     1621 gccattgata aaccagcttg ccgcatgagc accggccatc atcccgactt tccacaatcc
     1681 tggatgacta ttggcataag cgaacatttt tatcgcccgt tgctctgctt ttgcggtgat
     1741 ccctttttca gccatcaccc gacgatgacg caaaatcagt tttgacagcg gaatacgcac
     1801 cggacacacg ttgtcacaag ctgtgcataa agagcaggcg tagggtaaat ctttaaaatc
     1861 tttatagccg ccaagtagcg gagaaatcac cgcaccaatt ggccctggat aaatagagcc
     1921 atatccatga ccgccaatat ggcgatatgc cggacaagta ttcatacaag ccccgcagcg
     1981 aatacagcgc agcacatccc gaaattcaga ggccagcacc tcagaacgcc cgttatcgac
     2041 aataaccaga tgaaactctt caggaccatc aacgtgccca gcttcgcgcg gtcctgtcag
     2101 ccaggtgttg tatcccgtca aacgtgcacc aacggcactg cgcgccagca tggtgatcaa
     2161 tacatctacc tcggcaaacg tgggggcaat acgctccatt cccatcactg caatatgcgt
     2221 tttaggcagc gtggtacaca ttcgcgcatt accttcattg gtcaccaggc ataccgaacc
     2281 ggtctctgcc accgcgaaat tacagccggt aatacctatt tcagcactga ggaaatcttc
     2341 gcggattttt tgccggatga ataaggtcat cgcttcaggc gtttccggcc cctcatagcc
     2401 cagacgttcg tgtagcactc gacggatctg atggcgatct ttatgaattg ccgggaccac
     2461 aacatgagat ggcggatctt gatccagctg gagaatatat tcacccagat cggtttcaat
     2521 cacctgaatg ccagcatcct gcaacacatg attgacacca atctcttcgg tcaccatcga
     2581 tttagatttc accaccttcc gggcattttt gcgttgggca acctgtaaaa tgtagcgggt
     2641 agcgtcttct ttggttcttg caaaatagac gtgaccgccg ttttgcgtca ctttttctga
     2701 gagctggtac agataagcgt cgagattact cagaacatga tcacgtatct gggcggcccg
     2761 atcgcgccac tcctcccagt gccccaattc atcgaccatt ttttgccgat ttgccccaat
     2821 acgctgctgc gcgtttgcca ccgctttgcg catgatcgga tcttcaattt gctgacggat
     2881 gcgtgtctta aaatctgtat tactggtttt gatcgacatc tttatatcct cagcggctca
     2941 tcaacacttc agcaatatgc atcactttga ctttctgccc ttcccgttgt aatcgcccac
     3001 tgatgtttag caggcaactc acgtcagcac caattaaata ctcagggcgg acttccatca
     3061 ggtgcgcaac cttttctttc accatctcgc cggatatttc ggccattttg accgagaacg
     3121 tgccgccaaa tccgcagcag gtatcctgtt cagcaaaggt aaacagctcc agtccacgca
     3181 catttttcag cagcgtaagt ggctcgtcct tcactcccag cttacgggcc aggctacaag
     3241 atgggtgata caccgctctc ccttgcaaac tggcacctac atcgactacc cctaatttat
     3301 taacaataaa agaggtgaga tcctgcatac gcgcggcaac ctttgcggca cgtgatgccc
     3361 attcaggttc atccgccaga tacatcgggt aactttttac ggcataggtg caggagccag
     3421 ccggtgaaat aatgggatca tcgttatcct ccagcgcggc gatcagattt ttcatccctg
     3481 gaatcgcttc tttgatataa ccgctattga tcgcaggctg accgcagcat ccctgtttct
     3541 ccgggaaatt tacgcgacag ccgagttttt ccagtagcag cacggagtct cgtgccattc
     3601 ttgatttcag ggcgtcacca atacaggtga caaagaaatt gacattcaaa actaatactc
     3661 cattacttca tgcccattta tgcccgatgt ttccccccca ccgggggatc aatcatcaat
     3721 aaatatggcg ttctttttta attggctagc aacataccac ccctcatttg tatgacaaat
     3781 gataatcttt catccaaacg tcaaatgcaa taactaattt tatttctttc ggcatcgctc
     3841 acattttatt gggaaatcga cgatcatttt tggcataaga aatccatttt aaaatggcta
     3901 catcccttat acctattcag agctacttat tca
//
LOCUS       DBANUU010000261         3933 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000261 DBANUU010000000
VERSION     DBANUU010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.266"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..500)
                     /locus_tag="ACIE68_004679"
     CDS             complement(<1..500)
                     /locus_tag="ACIE68_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFX5910849.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPGTFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNI"
     gene            complement(512..865)
                     /locus_tag="ACIE68_004680"
     CDS             complement(512..865)
                     /locus_tag="ACIE68_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFX5910850.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(877..1272)
                     /locus_tag="ACIE68_004681"
     CDS             complement(877..1272)
                     /locus_tag="ACIE68_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFX5910851.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVIDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(1314..2339)
                     /locus_tag="ACIE68_004682"
     CDS             complement(1314..2339)
                     /locus_tag="ACIE68_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFX5910852.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            complement(2395..2727)
                     /locus_tag="ACIE68_004683"
     CDS             complement(2395..2727)
                     /locus_tag="ACIE68_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFX5910853.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVDADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(2737..>3933)
                     /locus_tag="ACIE68_004684"
     CDS             complement(2737..>3933)
                     /locus_tag="ACIE68_004684"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFX5910854.1"
                     /translation="RLTDAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVL
                     PVSGTLVSRTRALQPYSGMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDC
                     ADIIARVRDIKPVWALANDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAA
                     LEKQGVEITLIYSGSHKVDGNPYSHLPGDVRETLQSRMDATRRIFAQKVSAYTGLSVQ
                     AVLDTEAAVYSGQEAIDAGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTT
                     VSATASQADVTDVVQATEGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREE
                     QARVLAETPGMTVETARRILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVND
                     LLNTPV"
BASE COUNT          776 a         1222 c         1067 g          868 t
ORIGIN      
        1 tcaatattct gtttaaacgc cgtggtcagc ggcaccgcca tcgggatttt cactacatca
       61 atggggtaac ggtttttccc ggccacacgc tgcataacat gccaccggcc gtttttcagt
      121 tgctgaataa acgtgccggg aatacgacgg tttcccacca caagcacact gccgccacct
      181 ttcagggctg aacgctgccc ctttttacga cgcctgcgtc gggacaggac aacccgcgcg
      241 ttacccagct ttattacggg caaatccccc cggttaactt tgattctggc ctgcggattt
      301 ttgaccgtgg cccttttcag ccgggccctt tcctttacca gtttccggcg tacctttgtc
      361 tcacgggcaa cctgtgacgc cgactgcgat atcgcggatg acgcaacgcg gttaatggcc
      421 attgcggcgg caccgggcac cgccgttctg ctgatacggc tgaggttttc aacggcctgc
      481 tcaagacctt ttatggccat acatccccct ttcagcggcg acggttaacg gcaggcggta
      541 cgccccgccc aagccagaga tgacagcttc cgccatcatc cggcgaaatc cggtctatcc
      601 agaagttttc ctcaccgatg gtcagcgtgt cgccgcgccg caactgccgc acatcatcag
      661 tccggacaaa cagggacggg ctggagcctt caacgcgcac gccctgtccg gcatagctga
      721 tattttcagg gtcatcaaaa acaccacgta tcaccgcacc tgactgctca ccggatgtca
      781 tggtggctga cgttcccatg tacccgcgta tcgtttcatc ggcgcgggca atggcagcat
      841 cgaacaggtt atcgaaatca gccacagcgc ctcccgttat tgcattctgg ccaggccgcg
      901 ctctgtcatt tcagctgcca caccggcaga gacacggaac gccgttcccg gcagcacaaa
      961 tgccacagcc tcatcccgcg tggcgtgaag tgcatcagta tgcagcgtca ccagtgccac
     1021 aaccgtgacc agatcagccg tatcaatcac ggtatccggc tgcgctgata caacctcatt
     1081 ttcatgtccg gtcagcgcat tttccgggct gacagatgtg tcctgaccgg cagcgtcatc
     1141 cgtgtcatca agctcctctt ccagctctgc cacacggagt gccagttctt ctttcgtccc
     1201 cgtcaggctg acatcacggt tcagttgctc acccagcacc tgaagacggg caatcagttc
     1261 atctttcgtc atggactcct ccacagagag aaaatggccc cgaagggcca tgattacgcc
     1321 agttgtacgg acacgaacgc atcagggtca gccagcagca tcagcggtgc tgactgaatc
     1381 atggtgaact cacgcgccgg atcgccggtg gtcacccagt ttttcgggta gcgggcagag
     1441 gcgttaatac cttcgcgctg tgcgtccgca tcctgaatgc agccataggt gcgcagaccg
     1501 cgtgcctgag tgttccccag caccatcgtg ttgtccggaa ggaagttctt tttgacgtcg
     1561 ttttccacgt actgtccgga atacacgacg atcgccgtat cgccatacat ccccttatag
     1621 gacaccgctt cgcccaggtc ttttaccgct gtctccagct cggaatgaga gccgcgacgg
     1681 gtatccagct tctccttgac ggctttgaag gaacggaaca gcgcccagcc tttcggatca
     1741 aacacgatga tattcaccac accgctggcg ttcagcgcgt aggcttcgat atcgtcggtc
     1801 gggtcatacg tggacttgtc acgcttgctc cactccgtgc cgccggactg cgtgatgttg
     1861 ttcgccacac tgcggcccat atccacttca accggatcga aggcttcacc ggtcatggtg
     1921 tatttgccct taagcacggc agaaactgcc tgcatctctt cgacctgagc aatggccagc
     1981 tcttcgtctc gcatgttctg caggatgatg cgacggcggc ggtaagccgg gtccgccaga
     2041 ttctgtggat cttcatccgg caggcgacgc agggtcatct gcggattcac ctcatgcttg
     2101 ggcttgacat atcccggtgt aaattcagag gtggatccgc cacgggaacg gataacctca
     2161 ccggaaacaa tcggcgaaac gtacagcgcc atgtttacca gtcccggaat ttgtgagaga
     2221 tagactttct ccgtggtgaa gggatagctc tcacggaaaa agagacgcag aaacagcgga
     2281 tcaaacttaa atttctgctc atttgccgcc agcagctggg cggttgtgta catcgacata
     2341 aaaaaatccc gtaaaaaaag ccgcacaggc ggcctttagt gatgaagggt aaggttaaac
     2401 gatgctgatt gccgttccgg caaacgcggt ccgttttttc gtctcgtcgc tggcagcctc
     2461 cggccagagc acatcctcat aacggaacgt gccggacttg tagaacgtca gcgtggtgct
     2521 ggtctggtca gcatcaaccg ccagaatgcc aacggcagca ccgtcggtgg tgccatccca
     2581 cgcaaccagc ttacgggtgg aggtgtccag catcagcggg gtcattgcag gcgctttcgc
     2641 actcaatccg ccgggcgcgg ttgccgtatg tgccgggtca ctgttgccca gcggctggta
     2701 atgggtaaag gtttctttgc tcgtcataaa catcccttac actggtgtgt tcagcaaatc
     2761 gttaacggca tcagatgccg ggttacctgc agccagtggt gccggtgccc cctgcatcag
     2821 acgatccagc gcagtgtcac tgcgcgcctg tgcactctgt ggtgcagctg ccagaatgcg
     2881 gcgggccgtt tccacggtca taccgggggt ttcggccagc acacgtgcct gttcttcgcg
     2941 tccgtgagct tcctcacagt tgaggatccc cataatgcgg ctgttttctg ccgcaaccgc
     3001 agcggtgatc tgcgcgttca cgtccggctg cgccgcgctg gcgttttcgc cctccgtcgc
     3061 ttgcaccacg tcagtaacgt cagcctgcga agcagtggct gaaacagttg ttgattgagt
     3121 ctctttggtc attcgccctc ctgagagacg ggatttacgt gcatccagtg catcacgcat
     3181 aacggtgatc gcatcggtgc tgttgacaag ttcatcagcc agtccggcat caatggcctc
     3241 ctgaccgctg tacactgcag cctcggtatc cagcacagcc tgcacggaca ggccggtata
     3301 tgccgacacc ttctgcgcaa atatccggcg ggttgcatcc atccgggact gcagtgtctc
     3361 ccggacatca cccggtagat ggctgtaggg gttgccatcc accttatggc tgccgctgta
     3421 aatcagcgtg atttccacgc cctgtttctc cagcgcagcg ccgtaattac tgtgagccat
     3481 catgacgccg atggagcctg tccgggcggt ctgcgtgacc agacgccggg aggcggcgct
     3541 ggcaagcagc tgacctgcac tgcagttcat gtcgttggcc agcgcccata ccggctttat
     3601 gtcacgcaca cgggcgatga tgtcagcaca gtcaaatgct cccgccacca tcccgcccgg
     3661 tgtgtccata tcgagcagaa tgccgtccac catcggatcg ctggcagcct gttgcagacg
     3721 agcgataatg ccgttgtaac cggtcattcc cgaatacggc tgcagcgccc gcgtccggct
     3781 gaccagcgta ccggacaccg gcagcacggc gatgccgttc atgacctgat aactgcgggc
     3841 ctgtcgtggt ccgtcatcat caccggataa cgccagcgcc gcgggtgcct ctccggcagt
     3901 caggctgtcg ccggatactg catccgtcag gcg
//
LOCUS       DBANUU010000262         3918 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000262 DBANUU010000000
VERSION     DBANUU010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.267"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..500)
                     /locus_tag="ACIE68_004685"
     CDS             complement(<1..500)
                     /locus_tag="ACIE68_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFX5910855.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIRLGNARVVLSRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNI"
     gene            complement(512..865)
                     /locus_tag="ACIE68_004686"
     CDS             complement(512..865)
                     /locus_tag="ACIE68_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFX5910856.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWVDRVTPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(877..1272)
                     /locus_tag="ACIE68_004687"
     CDS             complement(877..1272)
                     /locus_tag="ACIE68_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFX5910857.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGLDTSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTARGLARMQ"
     gene            complement(1314..2339)
                     /locus_tag="ACIE68_004688"
     CDS             complement(1314..2339)
                     /locus_tag="ACIE68_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFX5910858.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSEAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     THARGLRTYGCIQDADALSEGINASPRYPKNWKTSGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            complement(2395..2727)
                     /locus_tag="ACIE68_004689"
     CDS             complement(2395..2727)
                     /locus_tag="ACIE68_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFX5910859.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMPDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(2737..>3918)
                     /locus_tag="ACIE68_004690"
     CDS             complement(2737..>3918)
                     /locus_tag="ACIE68_004690"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFX5910860.1"
                     /translation="VSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGT
                     LVSRTRALQPYSGMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIA
                     RVRDIKPVWALANDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQG
                     VEITLIYSGSHKVDGNPYSHLPDDVRETLQSRMDATRQIFSQKVSAYTGLSVQAVLDT
                     EAAVYSGQEAIDAGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATA
                     SQADVTGVVQATEGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVL
                     AETPGMTVETARRILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTP
                     V"
BASE COUNT          784 a         1210 c         1055 g          869 t
ORIGIN      
        1 tcaatatttt gtttaaacgc cgtggtcagc ggcaccgcca tcgggatttt cactacatca
       61 atggggtaac ggtttttccc ggccacacgc tgcataacat gccaccggcc gtttttcagt
      121 tgctgaataa acgcgccggg aatacgacgg tttcccacca caagcacgct gccgccacct
      181 ttcagggctg aacgctgccc ctttttacga cgcctgcgtc gggacaggac aacccgcgcg
      241 ttacccagcc tgattacggg caaatccccc cggttaactt tgattctggc ctgcggattt
      301 ttgaccgtgg cccttttcag cctggccctt tcctttacca gtttccggcg tacctttgtc
      361 tcacgggcaa cctgtgacgc cgactgcgat atcgcggatg aagcaacgcg gttaatggcc
      421 attgcggcgg caccaggcac cgccgttttg ctgatacggc tgaggttttc aacggcctgc
      481 tcaagacctt ttatggccat acatccccct ttcagcggcg acggttaacg gcaggcggta
      541 ccccccgccc aagccagaga tgacagcttc cgccatcatc cggcgtaacc cgatctaccc
      601 agaagttttc ctcaccgatg gtcagcgtgt ctccacgccg cagctgccgc acatcatcag
      661 tccggacaaa cagggacggg ctggagcctt caacgcgcac gccctgtccg gcatagctga
      721 tattttcagg gtcatcaaaa acaccacgta ttactgcgcc ggactgctca ccggatgtca
      781 tggtggctga cgttcccatg tacccgcgta tcgtttcatc ggcgcgggca atggcagcat
      841 cgaacaggtt atcgaaatca gccacagcgc ctcccgttat tgcattctgg ccaggccgcg
      901 cgctgtcatt tcagctgcca caccggcaga gacacggaac gccgttcccg gcagcacaaa
      961 tgccacagcc tcatcccgcg tggcgtgaag tgcatcagta tgcagcgtca ccagtgccac
     1021 aaccgtgacc agagcagccg tatcagtcac ggtatccgtc tgcgctgata ccacctcatt
     1081 ttcatgtccg gtcagcgcat tttccgggct gacagacgtg tccagaccgg ctgcgtcatc
     1141 cgtgtcatca agctcctctt ccagctctgc cacacggagc gccagttctt ctttcgtccc
     1201 cgtcaggctg acatcacggt tcagttgctc acccagcgac cggagacggg caatcagttc
     1261 atctttcgtc atggattcct ccacagagag aaaatggccc cgaagggcca tgattacgcc
     1321 agttgaacgg acacgaactc atcaggatca gccagcagca tcagcggtgc tgactgaatc
     1381 atggtgaact ctcgcgccgg atcgccggat gtcttccagt ttttcggata acggggagac
     1441 gcattaatac cctcactcaa tgcatccgca tcctgaatac agccataggt gcgcagaccg
     1501 cgtgcatgag tgttacccag caccatcgtg ttgtccggca ggaagttctt tttgacgccg
     1561 ttttccacgt actgtccgga atacacgacg atggccacat cgccatacat tcccttataa
     1621 gacaccgctt tgcccaggtc ttttaccgct gtctccagtt cggaatgaga gccgcgacgg
     1681 gtatccagct tctccctgac ggctttgaag gaacggaaca gcgcccagcc tttcgggtca
     1741 aacacgatga tattcaccac gccgctggcg ttcagcgcgt aggcttcgat atcgtcggtc
     1801 gggtcatacg tggacttgtc acgcttgctc cactccgtga cgccagactg tgtgatgtta
     1861 ttcgcttcac tgcggcccat atccacctca accggatcga aggcttcacc ggtcatggtg
     1921 tatttgccct tgagcacggc agaaactgcc tgcatctctt cgacctgagc aatggccagc
     1981 tcttcgtctc gcatgttctg catgatgatg cgacggcggc ggtaagccgg gtccgccaga
     2041 ttctgtggat cttcatccgg caggcgacgc agggtcatct gcggattcac ctcatgcttg
     2101 ggtttgacat atcccggcgt aaattcagag gtggagccgc cacgggaacg gataacctca
     2161 ccggaaacaa tcggcgaaac gtacagcgcc atgtttacca gtcccggaat ttgtgagaga
     2221 tagactttct ccgtagtgaa gggatagctc tcacggaaaa agagacgcag aaacagcgga
     2281 tcaaacttaa atttctgctc atttgccgcc agcagctggg cggttgtgta catcgacata
     2341 aaaaaatccc gtaaaaaaag ccgcacaggc ggcctttagt gatgaagggt caggttaaac
     2401 gatgctgatt gccgttccgg caaacgcggt ccgttttttc gtctcgtcgc tggcagcctc
     2461 cggccatagc acatcctcat aacggaacgt gccggacttg tagaacgtca gtgtggtgct
     2521 ggtctggtca gcagcaactg caagaatgcc aacggcagca ccgtcggtgg tgccatccca
     2581 cgcaaccagc ttacgggtgg aggtatccgg catcagcggg gtcattgcag gcgctttcgc
     2641 actcaatccg ccgggcgcgg ttgccgtatg tgccgggtca ctgttgccca gcggctggta
     2701 atgggtaaag gtttctttgc tcgtcataaa catcccttac actggtgtgt tcagcaaatc
     2761 gttaacggca tcagatgccg ggttacctgc agccagcggt gccggtgccc cctgcatcag
     2821 acgatccagc gcagtgtcac tgcgcgcctg tgcactctgt ggtgcagctg ccagaatgcg
     2881 gcgggccgtt tccacggtca taccgggggt ttcggccagc acgcgtgcct gttcttcgcg
     2941 tccgtgagcc tcctcacagt tgaggatccc cataatgcga ctgttttctg ccgcaaccgc
     3001 tgcggtgatc tgcgcgttca cgtccggctg cgcagcgctg gcgttctcgc cctccgtcgc
     3061 ttgcaccacg ccagtaacgt cagcctgcga agcagtggct gaaacagttg ttgattgagt
     3121 ctctttggtc attcgccctc ctgagagacg ggatttacgt gcatccagtg catcacgcat
     3181 gacggtgatc gcatcggtac tgttaacaag ttcatcagcc agtccggcat caatggcctc
     3241 ctgaccgctg tacactgcag cctcggtatc cagcacagcc tgcacggaca ggccggtata
     3301 tgccgacact ttctgcgaaa atatctggcg ggttgcatcc atccgggact gcagtgtttc
     3361 ccggacgtca tccggaagat ggctgtaggg gttgccatcc accttatggc tgccgctgta
     3421 aatcagcgtg atttccacgc cctgtttctc cagcgctgca ccgtaattgc tgtgagccat
     3481 catgacgccg atggagcctg tccgggcggt ctgcgtgacc agacgccggg aagcggcact
     3541 ggcaagcaac tgacctgcac tgcagttcat gtcgttggca agcgcccata ccggctttat
     3601 gtcacgcaca cgggcgatga tgtcagcgca gtcaaatgcc cctgccacca ttccgccggg
     3661 cgtgtccata tcgagcagaa tgccgtccac catcgggtcg ctggcagcct gttgcagacg
     3721 ggcaataatg ccgttgtaac cggtcatccc cgagtacggc tgcagcgccc gcgtccggct
     3781 gaccagcgtg ccggacaccg gcagcacggc gatgccgttc atgacctgat aactgcgggc
     3841 ctgtcgtggt ccgtcatcat caccggataa cgccagcgcc gcgggtgcct ctccggcagt
     3901 caggctgtcg ccggatac
//
LOCUS       DBANUU010000263         3799 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000263 DBANUU010000000
VERSION     DBANUU010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.268"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            122..1180
                     /gene="rfaQ"
                     /locus_tag="ACIE68_004691"
     CDS             122..1180
                     /gene="rfaQ"
                     /locus_tag="ACIE68_004691"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFX5910861.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1177..2301
                     /locus_tag="ACIE68_004692"
     CDS             1177..2301
                     /locus_tag="ACIE68_004692"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFX5910862.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2294..3091
                     /gene="rfaP"
                     /locus_tag="ACIE68_004693"
     CDS             2294..3091
                     /gene="rfaP"
                     /locus_tag="ACIE68_004693"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFX5910863.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            3107..>3799
                     /locus_tag="ACIE68_004694"
     CDS             3107..>3799
                     /locus_tag="ACIE68_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFX5910864.1"
                     /translation="MSAHYFNPQEMITKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKVLYLDADIACQGNLKPLITMDLANNIAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGK"
BASE COUNT          985 a          829 c          934 g         1051 t
ORIGIN      
        1 tgatacacta gcgcgtcaat atcagtgcgg tatcagtatt caggtagctg ttgagcctgg
       61 ggcggtagcg tgctttttct gcttcacctt aatcggataa tctcaacaaa aagagttact
      121 tgtggataag ccatttcgaa gaattttgct cattaagatg cgttttcatg gggatatgtt
      181 attaactact cccgtcatta gttcgctgaa aaaaaattac cctgacgcaa aaatcgatgt
      241 gctgctttat caggacacca tcccgatcct gtctgaaaat ccagagatta acgcgctcta
      301 cggcataaaa aataaaaaag caaaagcctc agaaaaaatt gccaactttt ttcatctcat
      361 caaggtatta cgtgccaata agtatgacct tatcgtcaat cttaccgatc aatggatggt
      421 tgctatactg gttcgcttat taaatgcccg tgtgaaaatt tcccaggatt atcatcatcg
      481 gcagtctgct ttttggcgta aaagtttcac ccatttggtg ccgttgcagg gtggaaatgt
      541 ggtggaaagt aacttatccg tgctgacacc attgggactt gattcgttgg tgaagcagac
      601 aaccatgagt tacccgcctg caagctggaa acgtatgcgt cgcgaacttg atcacgctgg
      661 tgttggacaa aattatgtgg ttatccaacc tacggcgcgg caaatcttca aatgctggga
      721 caacgccaag ttttccgctg tgattgatgc cttacatgct cgtggttatg aagtcgttct
      781 gacgtccggc ccggataaag acgatctggc ctgcgtcaat gaaattgcgc agggatgcca
      841 gacgccacca gtaacggcgc tggctggaaa ggtgaccttc ccggaacttg gtgcgttaat
      901 cgatcatgcg cagctgttta ttggcgttga ttccgcaccg gcgcatattg ccgctgcagt
      961 taatacgccg ctgatatcgc tgtttggtgc gacagaccat attttctggc gtccctggtc
     1021 aaataacatg attcaattct gggcgggaga ttaccgggaa atgccaacgc gcgatcagcg
     1081 tgaccgaaat gagatgtatc tttcggttat tccggcggca gatgtcattg ctgctgtcga
     1141 taaattactg ccctcctcca cgacaggtac gtcgttatga tcgttgcttt ttgtttatat
     1201 aaatattttc cctttggcgg tttgcagcgt gattttatgc gtattgctca gacagtcgcc
     1261 gcccgaggtc atcatgttcg ggtttatacc cagtcgtggg aaggcgaatg ccctgatgta
     1321 tttgaactga tcaaagtgcc ggttaaatcg cataccaatc acgggcgcaa tgcggagtat
     1381 tttgcctggg tgcaaaaaca tttacgcgaa catcccgtcg ataaagtcgt tggattcaac
     1441 aaaatgccgg ggctggacgt ttattatgcc gctgatgttt gttatgccga gaaagtagcg
     1501 caggaaaaag gctttttcta tcgcctgacg tcacgttatc gccattatgc cgcctttgag
     1561 cgggcaacct tcgaacaggg taagccgaca cagttgctga tgctgacaga taagcaaatc
     1621 gccgatttcc agaaacatta tcagactgaa gcggagcgtt ttcatattct gccaccgggg
     1681 atttatcctg atcgtaaata tagccagcag ccagccaata gccgtgaaat cttccgtaag
     1741 aagaatggaa taaccgaaca acaatattta ttgttgcagg tcggttcaga cttcacgcgt
     1801 aaaggtgtcg atcgttccat tgaagcactt gcttcgttac cggattcgct gcgccacaac
     1861 acattgctat atgttgttgg gcaggataaa ccgcgaaaat ttgaggcgct ggcagaaaaa
     1921 cgcggcgtgc gcagtaatgt tcactttttc tcggggcgca atgatgtctc ggaattaatg
     1981 gcggcggcgg atttattact gcatcctgcc taccaggaag cggcgggaat tgtgctgctg
     2041 gaagcgatta ctgcaggatt accggtacta acaacagccg tttgtggcta tgcgcattat
     2101 attgtcgacg ccaattgcgg cgaggctatt gctgagccat tccgccagga aacattgaat
     2161 gagattttac gcaaagcgtt aacgcaatct tcattgcgcc aggcctgggc ggaaaatgcg
     2221 cgacattatg ctgatacaca agatttatac agtctgccag agaaagcggc ggatatcata
     2281 acgggtggtc tggatggttg aacttaaaga gccgtttgcc acgttatggc gcggcaaaga
     2341 tccttttgag gaagttaaaa ccttgcaggg tgaggtattt cgtgaactgg aaactcgccg
     2401 tactctgcgc tttgaaatgg cgggcaaaag ctattttctc aaatggcatc gcggcacgac
     2461 cctgaaagag ataatcaaaa atttactctc attgcggatg ccagtattag gcgcagaccg
     2521 cgaatggaat gcgattcatc gactgcggga tgtcggcgtt gatactatgt atggggtggc
     2581 attcggcgaa aaaggcatga atccgctgac cagaacctcg tttattatta ccgaagatct
     2641 gacaccaacc ataagtctgg aagattactg tgctgactgg gcgactaacc ctccagatgt
     2701 tcgcgtaaag cgtatgctta ttaagcgtgt cgcgacgatg gtgcgcgata tgcatgctgc
     2761 gggcattaac caccgtgact gttatatctg tcatttcctg ctgcacttgc ctttttccgg
     2821 taaggaagag gagttaaaaa tttcggtaat tgacctgcac cgggcgcagc ttcgcacgcg
     2881 cgttccacgt cgttggcggg ataaagatct tattgggctt tatttttctt cgatgaatat
     2941 cggcctgact cagcgggata tctggcggtt tatgaaagtg tattttgccg ccccgcttaa
     3001 agacattctc aagcaggaac aaggactgct gtcgcaagca gaagcaaaag ccacaaaaat
     3061 cagggaaaga acgattcgaa aatcgttgta atttccggag agaaatatga gtgcccacta
     3121 ttttaatcca caagagatga tcactaagac aatcatcttc gatgaaaggc cagcggcgtc
     3181 agtggcatca tcattccatg ttgcttatgg cattgataaa aactttcttt ttggttgtgg
     3241 tgtttcaatc acgtcagttt tgttacataa caacgacgtg agttttgttt tccacgtttt
     3301 tattgatgat atccctgaag ccgatatcca gcgtttagcc caattggcga aaagctatcg
     3361 tacctgtatc cagatccatc tggtaaattg tgaacggctt aaggcattac cgacgaccaa
     3421 aaattggtct attgccatgt atttccgttt tgtaattgca gattacttta ttgatcaaca
     3481 agataaggtt ctttacctgg atgctgatat cgcctgtcag ggaaacttaa agccgctgat
     3541 aacaatggat cttgccaata acattgctgc tgttgttact gaacgcgatg ctaactggtg
     3601 gtcgttacgg ggtcaaagtc tgcagtgtaa tgaacttgaa aagggttact ttaattcagg
     3661 tgtcctgtta attaatacac tagcgtgggc gcaggagtcc gtttctgcta aagcgatgtc
     3721 gatgcttgct gataaagccg tcgtttcccg tttaacctat atggatcaag atatacttaa
     3781 tcttatcctg tcagggaaa
//
LOCUS       DBANUU010000264         3795 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000264 DBANUU010000000
VERSION     DBANUU010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.269"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(1..178)
                     /locus_tag="ACIE68_004695"
                     /pseudo
     CDS             complement(1..178)
                     /locus_tag="ACIE68_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein ImpB"
     gene            complement(239..343)
                     /locus_tag="ACIE68_004696"
                     /pseudo
     CDS             complement(239..343)
                     /locus_tag="ACIE68_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(345..668)
                     /locus_tag="ACIE68_004697"
                     /pseudo
     CDS             complement(345..668)
                     /locus_tag="ACIE68_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1410..2387
                     /locus_tag="ACIE68_004698"
     CDS             1410..2387
                     /locus_tag="ACIE68_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFX5910865.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(2672..3412)
                     /locus_tag="ACIE68_004699"
     CDS             complement(2672..3412)
                     /locus_tag="ACIE68_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFX5910866.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(3533..3721)
                     /locus_tag="ACIE68_004700"
     CDS             complement(3533..3721)
                     /locus_tag="ACIE68_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFX5910867.1"
                     /translation="MASDTFSCRRRLSAGFFSVSACSWHYPNSPKNGKPVLSPHHDWL
                     KTGSLRQNAPLRIWFYPQ"
BASE COUNT          922 a          958 c          937 g          978 t
ORIGIN      
        1 aagcttttcc acagcggcat tgctgtaaca gggttcaggg ggatgtccgt acaaatgtcg
       61 tcacctgccg gcaatactga cgctccccac gtagtttctc tgcggcccgc tctgcatact
      121 gaacaacagc ctggtgcatg gcatctttgt ctgtgattcg ttcaccaaaa ctgcgactat
      181 gtcaacgcca cgatgtttga ccgttatttg ccattttcat gcccgctaaa tactttcgtt
      241 actgatgctg tccgctttgc accatttcat catcactgat tggcgttgtg tttttggtca
      301 gcactccagc tttacgtttc tcttttaacc ggtaactctc tccttgttct ttttgacgga
      361 cgcattttac gtttgggctg gatgtagtaa cgcagcatgg aacgtccgcc cgtataaccc
      421 attgccttaa tctccgcgaa gataacctcg ctattccaga cattctctgc caggcgcatg
      481 tcgatgtaat ccataaacgg tttcagctta accattttgt ggcgggtctt tctggctggc
      541 ggttcagggt atttgaggta gcgtctgacc gtccgttcag agcaaccaat ctgagccgca
      601 atatcgacaa tgtacgcgcc ctgctggcgc atttgcttta tcatgtaaaa gtcctctctg
      661 ctcagcatgt tgatgtcctt tctggtgtga gaaccttaag gaaacaacat gttgggtgga
      721 gcggacaatc caaatggtga attaccgtct tatatcactg gcgctgacac ctgtagtttg
      781 tatacacaat ccctggcatt gaaacacaca tatgaagtgt acttcggata tgaccgtacg
      841 ctcaggaagt acggcagatt ccgtcaaaaa ttacctgtaa agacagacat cagaaaaaca
      901 gtgctcgccg cagaccggtg accgggcgaa gcccgcgaac cggcgaggaa gcgcaatgga
      961 tctgtagtta cagtgagcag gtacgccctg aagccttcat aatcggtagt gtatgctgtt
     1021 tttctggaat gatttatcca catatccgga ggctcgatcc agtaaacaga tccatgaatg
     1081 atcaacaaag gatccattaa agatccccat atcgctgcaa accttgtcat taatggtccg
     1141 ggagcacgat cacataagct gtggcatgtt actgataaac tgtaacaagt taatgataag
     1201 ctgtattaag taatacatat actgcagtaa gttaatgaca taaactatgg tcagtacgcc
     1261 agactcagct gttaaataca ggctgcagat ttttcttcag tcagttagcg gggttctgac
     1321 atccgatttt ctgtttattc ttttactgtc cacaggcagg aggctttctg gaaaacgaaa
     1381 attcagacat caaaaaactg ttcggcgagg tggataagtc gtccggtgag ctggtgacac
     1441 tgacaccaaa caataacaac accgtacaac ctgtggcgct gatgcgtctg ggcgtttttg
     1501 taccgaccct taaatcactg aagaacagta aaaaaaatac actgtcacgt actgatgcca
     1561 cggaagagct gactcgtctt tccctggccc gtgctgaggg attcgataag gttgagatca
     1621 ccggcccccg cctggatatg gataacgatt tcaagacctg ggtggggatc attcattcct
     1681 ttgcccgcca taacgtgatt ggtgacaaag ttgaactgcc ttttgttgag tttgcaaaac
     1741 tgtgtggtat accttcaagc cagtcatccc gcaggctgcg tgagcgcatc agcccttccc
     1801 tgaagcgcat tgccggtacc gtgatctcgt tttcccgtac cgatgagaag cacacccggg
     1861 aatacatcac ccatctggta cagtcagcct actacgatac tgaacgggat attgttcagt
     1921 tacaggctga tccccgcctg tttgaactgt accagtttga cagaaaggtc cttcttcagc
     1981 ttaaggcgat taatgccctg aagcgacggg agtccgccca ggcactctac acctttatag
     2041 agagcctgcc ccgggatccg gcaccgatat cgctggcgcg gctgcgtgca cgcctcaatc
     2101 tgaagtctcc tgtattttcc cagaaccaga cggtcagacg ggcaatggag cagctgcgcg
     2161 agattggata tcttgattac acggagatcc agcgggggcg gacaaaactc ttctgtattc
     2221 actaccggcg tccccggtta aaagcgccga atgatgagag taaggaaaat ccgttgccac
     2281 cttcacctgc ggaaaaagtc agtccggaga tggcggagaa acttgccctg cttgaaaaac
     2341 tgggcatcac gctggatgac ctggaaaaac tcttcaaatc ccgctgaaca taaactgtag
     2401 tcagtgaaga gcgctccttt actgactaca gcttatatta actggtgcgg tgagtggtct
     2461 gctcactgca gtttatattc tgttccctgc agtgctgtct gtagctgagc tgccatctgc
     2521 ctgtccctta cgtaagtgat cccgtaacct gatgctgagg cattgctccc ttcataaaac
     2581 atgacttact cactacagct tatatacatg ctccagctta tgttatgtct gttctgctga
     2641 ccacagctta tataaggacg cgcgtatgcc gtcacctcag gggaggcagt gtacgcagga
     2701 tctccgcagc atcccgtccg tcacctgtga aaggcactgc cagcgtggca gccatatcca
     2761 gcgcaaaaac tctggtataa acctccatag aacgtggatc cctgtgacca gccagtgcct
     2821 gaatgacttt ccggggctgg cggtgataaa gcatgtgcat gatatagctg tgccggaagg
     2881 tatgtggtgt gaccggaatc gaaaagtgta ctccgtcagc ttcggcccgt ctgacagcct
     2941 gcttcagcca gttgcgcatg gtttcgtcgg tcacggccca taatggttca cgacgacggg
     3001 gccgggtggt gatcatccag ctttccatct gcctgacata gcttatatcc gtcagcggaa
     3061 ccaggcggac ttcatctttt ggcgggcgtc cgcgtcgcgc acgcactttt tcggacagga
     3121 tccgcacaaa cggtcttact ccatccaggt caaatgattc cggcgtcagc atccgggctt
     3181 cgccaatacg cattccggta ttccagaggg tggcgaacag catatggtga cgctgatccg
     3241 gcatatagaa aagaagggca ctcacttccg gggccagcag gtaggccggt gtggcccctg
     3301 tggaagtggc cattcttctc agggagagcg ctgttgcaaa atccaccccc ggcgcaatgg
     3361 gtaacaggga gacgcgttct ggtgaattct gcaggggaat gacattgttc atgagttatt
     3421 tttcacgtag cacacgcact gtttatggat acaggtattc tgacctgaac gcccggatcc
     3481 ttccacgaca ataaatcgca gagcgcggat ccttttgttt gcttatcacg cattattgcg
     3541 ggtaaaacca aatacgtaaa ggcgcgtttt gccgcaggga tcctgtctta agccagtcat
     3601 ggtgcgggct gaggactggt ttgccgtttt tgggagaatt ggggtaatgc caggaacagg
     3661 cagatacgga gaaaaatcct gcagataacc ttctacgaca actgaaggtg tctgacgcca
     3721 tattcttagc tacttctcag ttttaaggcg gcttagtcac aacataaggt tatgcataat
     3781 aagcatattt tatta
//
LOCUS       DBANUU010000265         3772 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000265 DBANUU010000000
VERSION     DBANUU010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.270"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..564)
                     /locus_tag="ACIE68_004701"
     CDS             complement(<1..564)
                     /locus_tag="ACIE68_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910868.1"
                     /translation="MFGMSITQSDGSLWMSPEFTPQNLINKGTMSTSKGSVFQTSIPS
                     NKSCFFFIKSSNKANMMFIHEHSNGYNALRLHQVNGSPGTITVYAFSDMVLPHSGYGI
                     AMYNSAGAMVYHGEMMPLDAKLITISDPQFTIDMGYPCAVMPAMVGVYNYRRTDYDRP
                     VYVTMTGATGNQVYNGQWYSGNVTWDIK"
     gene            complement(567..848)
                     /locus_tag="ACIE68_004702"
     CDS             complement(567..848)
                     /locus_tag="ACIE68_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910869.1"
                     /translation="MAYGTGIYNNKGINVTGFLTPIFFLDRFTASSGSKTYSNPPPGK
                     SLHAVWSLMPLNNDNYINLPVPTVTINGNTVSWSNLYTGLGSYIYTYWG"
     gene            complement(904..1473)
                     /locus_tag="ACIE68_004703"
     CDS             complement(904..1473)
                     /locus_tag="ACIE68_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910870.1"
                     /translation="MCKKCKAIADEQNALFEEMDANELVKMLAILRGIEDVSIFERVV
                     TALNFESTFEEPTQVVALAHHFGVHYLAEKERADKLQATLDMVSETQRTDDTNKSEAI
                     IASKDREIAGLKSSLTMLMSAFNLMSSQAGYKMPSLNSDDPMAVRQLLGAMADQLDDT
                     KSRLEDMMRELSHRHNLATQPHKAFQSSN"
     gene            complement(1613..1771)
                     /locus_tag="ACIE68_004704"
     CDS             complement(1613..1771)
                     /locus_tag="ACIE68_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFX5910871.1"
                     /translation="MLERFRLVTVIALLVMAVLVDFTGKMMSVISDGVLIGLAIYFAY
                     PLVRKATC"
     gene            complement(1771..2196)
                     /locus_tag="ACIE68_004705"
     CDS             complement(1771..2196)
                     /locus_tag="ACIE68_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFX5910872.1"
                     /translation="MLGLFKKKTRKAVIEVKKMENRDAVEATVWGAYMISYADGTCDA
                     KEISILEKTIAALPAFSPFAGEIAQMSANIRAQYEASPRRANAQALRELADVAGTNDA
                     VDVLCLCLDIADQDGIGEQEELALKKIAQALQLSLDAYL"
     gene            complement(2290..2487)
                     /locus_tag="ACIE68_004706"
     CDS             complement(2290..2487)
                     /locus_tag="ACIE68_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910873.1"
                     /translation="MNWFSNHFGKTWLAILAFIALMAAGWVSNIVKLVCSGDLQFQAG
                     MTLARVVGIFVFPVGSVLGYF"
     gene            complement(2497..2991)
                     /locus_tag="ACIE68_004707"
     CDS             complement(2497..2991)
                     /locus_tag="ACIE68_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910874.1"
                     /translation="MARQTYFTSATKRPRSLRQILAELFSGRVLSRLDELETAVRLLN
                     ERLDKQASVVANVGAIVTSGSSREAKSTRPLVKEKNNKDSSNGKFSKKEAETNGLRSH
                     YSFTGDGSRSSRPEPFDAGFIHHHTSVDDNYHHSSRASCHSGWDDGGCDTSSSSSYSG
                     SCCD"
     gene            complement(3137..>3772)
                     /gene="rnhA"
                     /locus_tag="ACIE68_004708"
     CDS             complement(3137..>3772)
                     /gene="rnhA"
                     /locus_tag="ACIE68_004708"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFX5910875.1"
                     /translation="RGKKIGAALDSGVYMTTKTPARSQEKTRKKAKNKNSPRTNSTTP
                     VVEFNPQLKTVKIFSDGSCLKNPGGPCGYGIVLQYRGEEREFSDGFHSTTNNRMEMMG
                     ALIGLERLKYPCNVILHSDSQYLKNGMTQWMKWWKRNGWVTSDKKPVKNVDLWKRLDE
                     AASRHNVRWKWVKGHAGHRENEICDRLAKIAAFSAADMPHKKDIGFVYNKQ"
BASE COUNT         1029 a          887 c          793 g         1063 t
ORIGIN      
        1 cttaatatcc catgtgacat taccggaata ccattgaccg ttatatactt ggtttccagt
       61 tgcacctgtc attgttacat agacaggtct gtcgtaatcg gttcgtctat agttatatac
      121 cccaaccata gcaggcatta ccgcacatgg ataccccata tctattgtaa attgagggtc
      181 agaaatagtg ataagtttcg catcaagggg catcatctca ccgtgataca ccattgcgcc
      241 tgcgctgtta tacatggcaa tgccatagcc agaatgtggt aacaccatat cagaaaaagc
      301 ataaactgtt attgttccgg ggctaccatt tacctgatgc aatctgagag cattgtatcc
      361 gttactatgc tcatgaataa acatcatgtt cgccttatta ctgcttttta tgaagaaaaa
      421 gcatgatttg tttgatggga ttgatgtttg aaaaacagaa cctttagatg tagacattgt
      481 ccctttatta atcagatttt gcggagtaaa ttctggactc atccacaaac ttccatctga
      541 ttgagtaatt gacatcccga acataattat ccccagtatg tgtatatgta agaaccaagc
      601 cccgtatata aatttgacca actaaccgta tttccattaa tagtaactgt tggaacaggt
      661 aaattaatgt agttgtcgtt gttaagaggc atcaatgacc acacggcatg tagcgattta
      721 cccggtggcg gattggagta cgtcttagag cctgatgacg cagtaaatcg atcaagaaaa
      781 aaaataggag taaggaagcc agtaacgtta attcctttat tgttgtaaat ccctgtaccg
      841 tatgccattt ttcatccttg ttttttatca acacaatatg gccgatcatg cggccatatc
      901 agatcaatta gagctttgaa aggctttgtg tggctgtgtt gcgaggttat gtcgatggct
      961 taactcacgc atcatgtctt caagccgact ctttgtgtcg tcgagttggt cggccattgc
     1021 tcccaaaagc tgacgaacgg ccatcggatc atcgctgttt agtgatggca ttttataacc
     1081 cgcctgagaa gacataagat tgaacgcaga catcaacatc gttagagagg atttaagccc
     1141 agcaatttca cgatctttgc tggcaataat cgcctcactc ttgttagtgt catcagttcg
     1201 ttgagtctcg ctcaccatat ccaaagtcgc ctgcaactta tcagcgcgct ctttttcagc
     1261 aagataatga acaccgaaat gatgtgctaa agctacaacc tgagttggct cttcaaaggt
     1321 ggactcaaaa ttaagtgctg taaccactct ctcaaatatg gaaacgtctt ctattcctcg
     1381 aagaatggct aacattttga ccagttcatt agcatccatt tcttcgaata aggcgttttg
     1441 ttcatcagct atcgctttgc atttcttgca catgtgtttt tcctaatgct taaacaagtt
     1501 gttttcttat tggctttatt gtgatgtgac ataaaagggg agcaagctaa acgtaaaggt
     1561 gcaaactctc tatttcggtc aacgaaacac aagggccatt tggcccttgt cattaacacg
     1621 ttgctttacg gactagcgga taagcgaagt agatcgccag accaatgagg acgccatcag
     1681 aaatgacaga catcatcttt cccgtaaaat ccaccaacac cgccatcacc agaagagcaa
     1741 tgacggtcac aagccggaat ctctcaagca ttagagataa gcatccagtg acaactgaag
     1801 cgcctgtgcg atctttttca gggccagctc ttcttgttcg ccaataccgt cttggtcggc
     1861 aatatcaagg cacagacaca gtacatctac tgcatcatta gtcccggcca catcagccag
     1921 ctcacgtaaa gcctgggcat tagcgcggcg cggtgaggct tcgtattgag cacggatatt
     1981 ggcgctcatt tgggcaatct caccagcaaa cggagaaaac gcaggcagag ctgcaattgt
     2041 tttctcaaga atggaaattt cctttgcgtc gcatgtgcca tcggcatagg agatcatgta
     2101 cgcgccccac acggtggctt caaccgcatc tcggttctcc atttttttga cctcgataac
     2161 agctttacga gttttctttt tgaataaacc taacatgtgc tttccttttg ttatttgtta
     2221 aaacaagttg ttttctagtt acatgaattg cttacgaaaa gacggtcaat gcatatgcac
     2281 taacaaccgt cagaaataac caagtaccga accgactgga aaaacaaaaa tcccaacaac
     2341 acgagccaag gtcataccag cctgaaactg gagatcacca gagcaaacga gttttacaat
     2401 gttcgatacc caaccggcgg ccatgagagc tatgaaagcc agaatcgcca gccaagtttt
     2461 tccaaagtga tttgaaaacc agttcataca accgccttag tcacagcatg atcctgagta
     2521 actggatgaa cttgaggtat cgcatccacc gtcatcccat ccagagtgac aggacgctct
     2581 actggagtgg tggtaattgt cgtcgacgga ggtgtggtga tggatgaacc cggcatcaaa
     2641 aggctctggc cggctggaac ggctaccgtc gccagtgaaa ctataatgag aacgtaggcc
     2701 attggtttca gcctctttct ttgaaaattt tccattcgaa ctgtccttgt tgtttttctc
     2761 cttcactaaa ggccgtgtac ttttcgcttc gcgtgaagaa ccagaggtaa caatcgcccc
     2821 cacgttcgca acaactgacg cttgcttatc taaacgttca ttcagcaacc gaacggcagt
     2881 ctctagttca tcaagacgtg acagaacacg accgctaaat aattcggcca aaatttgacg
     2941 tagtgaacga ggacgtttag ttgcagaagt gaaataggtt tgacgtgcca tgtggactcc
     3001 atccagtgtc agaaagagtt gcggctggcg attgccagcc gcctttctcg ttccatcctg
     3061 gaactgtgtc ttaccgacac attgtcatcc tgacgccgat aagatacatg atttaaaatg
     3121 ataggtaaac acttacttac tgcttgttgt aaacaaaacc aatatctttc ttgtgaggca
     3181 tatctgctgc ggaaaaagct gcgatcttcg cgagtcgatc acatatttca ttttcacgat
     3241 gcccggcgtg acctttaacc cacttccagc gaacattatg tcgacttgca gcctcatcca
     3301 gacgcttcca cagatcaaca ttctttaccg gttttttgtc agaagtcacc catccattgc
     3361 gtttccacca tttcatccac tgtgtcatgc cgtttttcag atactggcta tcagagtgca
     3421 aaataacgtt gcatggatat ttcaaacgct ccagcccgat aagtgccccc atcatctcca
     3481 tgcggttatt ggtggtgcta tgaaaaccat ctgagaactc gcgttcctca ccacgatact
     3541 ggagaactat accgtaaccg cacgggccac ccggattttt aaggcaagag ccatcactga
     3601 agattttcac ggttttaagc tggggattga actctacgac aggcgttgtg gaattggtgc
     3661 ggggagaatt tttgtttttg gcttttttgc gagttttttc ttgcgatcgg gctggtgtct
     3721 tcgtcgtcat ataaactcct gaatcaagcg ccgcgccgat tttttttcct cg
//
LOCUS       DBANUU010000266         3758 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000266 DBANUU010000000
VERSION     DBANUU010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.271"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..901)
                     /locus_tag="ACIE68_004709"
     CDS             complement(<1..901)
                     /locus_tag="ACIE68_004709"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFX5910876.1"
                     /translation="MKKFDVAALGSGNIDIFLSIPSLPTRGGKVVGTRLGEQAGGTVA
                     NSACAMGQLGLNVVSVSCVGNDHSASIILDGFKKYHVNCDFVQVIPELIANTAIIFID
                     ELGEKTLVYSPGSDHEWDKEKALQAIAQSRYFYTMPADIEKFRMLAEYSRSQMTKVVV
                     DIEPHIIATPEHLARILHLADIAIFNYDGFIRGYATEPDFTLLHDIQDEYQLDAVVVT
                     LDVRGVIAVKGNEQVKIGSYTIPVIDTTGAGDTFNGAFVYSLIKNMPLIEALKFASAT
                     AAINITALGARGHLPTPSEVNLFL"
     gene            complement(906..1904)
                     /locus_tag="ACIE68_004710"
     CDS             complement(906..1904)
                     /locus_tag="ACIE68_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFX5910877.1"
                     /translation="MNMPARTPVMPLAQTREEINATAASLRHLAAIWGECFSTFQLDK
                     RAVERVCIVGSGDSWTVALCAAAWLGKYTHLFCYALQTWDFLQTDLTRYQKETLIIIL
                     SASGRPSMTVDALCHAVCSNAQVLGVTNFPGTPFCAITENMLYTWANKQGIPTQSSSV
                     TLYALLRLAQKLCPGLTPLQIEEDLEGKFSQINQHWQQKKRHFYQQKEITFLGSGLSW
                     GLAISGSNLLSCGPQIRATALPLEEFYHSLRLHQAGVGQHYILLPATSCESPFYLATQ
                     KAIVEQGATAELISFIPDASESNNLFLVMQWLYEMCWHLSCDYVDAGGQRVSHREK"
     gene            complement(1904..2980)
                     /locus_tag="ACIE68_004711"
     CDS             complement(1904..2980)
                     /locus_tag="ACIE68_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFX5910878.1"
                     /translation="MNKIEQAVIYNKILGSLACAGMGDALGAATELYSIDEIKAQWGG
                     FLNAFVSPPADTFAGSLNGIAGLITDDSSQMYVFSEGLVEAGFDNFTNNDWLACLLRW
                     ADMQPYANYKGPTTEQIVKALKEGRPTNTIGRIGTSSRQAPNVGTTNGAGMRVAPAGL
                     IWPGKKEKACHLALITCLPSHDTNIAIASACAIAAATSQAMLPEASLTSLLDAAIWGA
                     NYGERLAKQYARCVAGPSIAMRIQLAADIARRANDLESCLREMEGLVGNSVAAHESIP
                     AAIGLLLYCKGEPWETIHACANIGNDTDSIATMAGAIAGAWRGFDALPEDKYAFFRAV
                     NNKDFDIEAIASGLTLLALQAQEK"
     gene            complement(2994..3746)
                     /locus_tag="ACIE68_004712"
     CDS             complement(2994..3746)
                     /locus_tag="ACIE68_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFX5910879.1"
                     /translation="MIRIYKASKGQLKHREIFDEIKKKIDNGEWKEGKAIPTERELAE
                     HFLSSRTTIRKAIESLKQRGYLHSVHGQGTFTLPARSRENHLLHSFTDDIKARGGVPA
                     QNILEIGYIPLSDIIRKNLELDIHVHTVFCIKRIRYMGSTPLGIQTSWLALNDGQEIT
                     QEELLATGSLYILLEEKLAIKPLEATEIISARLPSPLERQLLELTADDVVLSCTRVTL
                     SVERKPMEYVEMVYPASRYSYKARITKDSFSV"
BASE COUNT          997 a          874 c          811 g         1076 t
ORIGIN      
        1 aaagaaaaag attaacctcc gatggtgtcg gcagatgacc tctggcacct aaggcagtaa
       61 tatttattgc tgctgtggca gaagcaaatt ttagtgcttc aattaagggc atgtttttta
      121 ttaagctata aacaaatgca ccattaaaag tatctccagc gcctgtggta tcaataacgg
      181 gaattgtata actacctatt tttacttgct cattgccttt gactgcaatt actcccctaa
      241 catccagcgt aaccacgaca gcgtcaagtt gatactcgtc ttgtatatca tgcagaagtg
      301 taaaatctgg ctctgttgca tagccacgaa taaaaccgtc ataattaaat atcgcaatat
      361 cagcaaggtg taaaattctg gcaaggtgtt caggtgttgc aatgatatga ggctcaatat
      421 caacgacaac ttttgtcatt tggctacggg aatattcggc cagcatacga aatttctcga
      481 tatctgctgg catcgtataa aaataacggc tttgcgcaat tgcctgcagg gctttttctt
      541 tatcccactc atgatcacta ccaggcgagt aaaccaacgt tttttcacct aattcatcaa
      601 taaagataat cgcggtattg gcaatcaatt ccggaattac ctgaacgaaa tcacaattta
      661 catgatattt tttaaatcca tcaagaatga tcgaggctga atgatcattg cccacacatg
      721 aaacagatac cacattgaga cccagttgtc ccatcgcaca ggcactgttg gcgactgtgc
      781 cgccagcttg ttcacccaga cgtgtcccga caactttacc gccacgagtg gggagtgacg
      841 ggatagataa aaaaatatct atatttccac tgcctaaagc ggcgacatca aatttcttca
      901 tagtcttatt tctccctatg gctaacgcgt tgcccgcctg cgtcgacata atcacaactt
      961 aaatgccagc acatttcata taaccactgc atgaccagaa ataaattgtt ggactcactg
     1021 gcgtcaggta taaaactgat taattctgct gtcgctccct gttcaacaat tgctttttgg
     1081 gttgcaaggt aaaatgggga ctcgcaggat gtggctggta gtaaaatata atgctgcccg
     1141 acaccagcct gatgtaagcg taatgaatga taaaattctt ccagtggaag ggctgtcgca
     1201 cgaatctgtg ggccgcaaga aagtaaattg ctccccgata ttgccagtcc ccaacttaag
     1261 ccactcccaa gaaacgttat ctctttttgt tggtaaaaat ggcgcttctt ttgctgccag
     1321 tgctgattaa tctggctaaa tttcccttcc aaatcctctt caatctgcag tggtgttaag
     1381 cctggacata atttttgcgc taagcgcagg agggcataaa gtgtgacgct actactttgc
     1441 gttggaatac cctgcttatt cgcccatgta tagagcatgt tttctgttat tgcgcaaaat
     1501 ggtgttccag ggaaattcgt cacacccagc acttgcgcgt ttgagcatac tgcgtggcag
     1561 agggcgtcaa cggtcattga tggtctgcca cttgcactga gaataatgat taacgtttct
     1621 ttctgataac gggttaaatc agtctgaaga aaatcccacg tttgcaatgc gtaacaaaac
     1681 agatgtgtgt atttccccag ccaggcggcg gcacatagtg ccaccgtcca ggagtcgccc
     1741 gatcccacta tacagacgcg ttcaacagca cgtttatcga gctgaaaagt agaaaaacat
     1801 tcaccccaga tagctgccaa atggcgtaac gatgccgccg tagcatttat ttcctcacga
     1861 gtttgcgcta atggcataac aggagtacgg gctggcatgt tcattatttc tcctgtgcct
     1921 gtaatgcaag caatgtaaga cccgatgcga tcgcttcaat atcgaaatct ttattattta
     1981 cggcgcggaa aaaggcatat ttatcttcag ggagcgcatc aaaaccgcgc catgcccctg
     2041 caattgcgcc tgccatcgta gcgatgctat cggtgtcatt gccaatattg gcgcaggcat
     2101 gaatggtttc ccagggttct cctttgcagt acagcaatag accgattgcc gcaggaatgg
     2161 attcatgggc ggcaacagag ttaccaacca aaccttccat ttctcgcaga caagactcca
     2221 ggtcattggc tcgtctggcg atgtccgctg caagttggat gcgcatggcg atagatggcc
     2281 ctgcgacaca tcgtgcatat tgtttggcca ggcgttcgcc gtaattcgct ccccagattg
     2341 cggcgtccag taatgatgtg agcgaggctt ctggtaacat agcttgtgat gtcgccgcag
     2401 cgatggcgca agcgctggca atcgcaatat tggtgtcatg cgaaggtagg caagttatta
     2461 gcgccaggtg acacgctttt tctttcttgc ctggccagat caatcctgcc ggggctacac
     2521 gcattcctgc gccgttcgtg gtgccgacat tgggtgcctg acgagaagat gttccaatac
     2581 gcccaatggt gtttgttggt ctaccttctt ttagtgcctt gacgatttgt tctgttgtgg
     2641 ggcctttata atttgcgtag ggctgcatat cggcccaacg cagaagacaa gctaaccagt
     2701 cattattggt gaagttatca aatccagcct caactaaccc ttctgaaaat acatacattt
     2761 ggctggaatc gtcggtaata agtcccgcaa taccatttaa tgagccagca aaggtatcgg
     2821 caggaggaga aacaaaggca tttaaaaaac ctccccattg cgcttttatt tcatcaatgc
     2881 tgtagagttc agtcgctgcg cctaatgcat cgcccatacc cgcgcaggct aaagaaccta
     2941 atattttgtt ataaattacg gcttgttcta ttttgttcac aatattctct ctgttacacg
     3001 ctaaaactgt cttttgtaat tcgtgctttg taggagtacc gacttgcggg ataaaccatc
     3061 tctacatact ccattggttt gcgttccacg gataacgtga cgcgggtaca acttaatacg
     3121 acgtcatcgg cagtcagttc cagtaattga cgctcaagag gcgagggaag tcgggcagag
     3181 ataatttccg ttgcttctaa aggtttaata gcgagttttt cttccagtaa aatatagagg
     3241 gaacccgtcg ccagcagctc ttcctgagtt atttcttgtc cgtcatttaa cgcaagccat
     3301 gatgtctgaa tacccagcgg cgtacttccc atatagcgaa tgcgttttat acagaaaacg
     3361 gtatggacat ggatatcaag ttcgagattt tttctgataa tatcacttag aggaatataa
     3421 cctatttcaa gaatattctg tgcaggtacg ccgccacgag ccttaatgtc atcagtaaaa
     3481 ctatgtaata aatgattttc ccgactgcga gccggtaatg taaatgttcc ttgcccgtgg
     3541 acactatgca ggtatccgcg ttgtttcaaa ctttctattg ctttccgaat tgttgttctg
     3601 ctgcttaaaa aatgttcagc aagttctctt tctgttggta tagctttgcc ttctttccat
     3661 tcaccgttat caatcttttt ctttatttca tcaaaaattt cacgatgctt aagctgacct
     3721 ttactggctt tataaattct tatcatgaca aagcctca
//
LOCUS       DBANUU010000267         3740 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000267 DBANUU010000000
VERSION     DBANUU010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.272"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..216
                     /gene="ypdI"
                     /locus_tag="ACIE68_004713"
     CDS             <1..216
                     /gene="ypdI"
                     /locus_tag="ACIE68_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFX5910880.1"
                     /translation="VFAFSISGGVSERSYKETEKTSAMTTTHSTKLQPSQAILFKMRE
                     DAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(293..535)
                     /gene="yfdY"
                     /locus_tag="ACIE68_004714"
     CDS             complement(293..535)
                     /gene="yfdY"
                     /locus_tag="ACIE68_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFX5910881.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            888..1808
                     /gene="lpxP"
                     /locus_tag="ACIE68_004715"
     CDS             888..1808
                     /gene="lpxP"
                     /locus_tag="ACIE68_004715"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFX5910882.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2164..2235
                     /gene="ypdK"
                     /locus_tag="ACIE68_004716"
     CDS             2164..2235
                     /gene="ypdK"
                     /locus_tag="ACIE68_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFX5910883.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2300..3538)
                     /gene="alaC"
                     /locus_tag="ACIE68_004717"
     CDS             complement(2300..3538)
                     /gene="alaC"
                     /locus_tag="ACIE68_004717"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFX5910884.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT          977 a          953 c          875 g          935 t
ORIGIN      
        1 gtttttgcat tttccatttc tggtggtgta agtgagagga gctataaaga gactgaaaaa
       61 acatcagcga tgacgaccac acactctaca aaacttcagc catcacaggc gattttgttt
      121 aagatgagag aagatgcgcc accattaaac ctcacagaag aaatcacgcc tacttatccg
      181 acaaaggcga attatcttat tcatcctgtg cgatagcgtc tgtcacatgg caggtgaaca
      241 atcatgtctg atgctgatgt gaacacctgc ccaaatgagc catcctgacc cactatcgcc
      301 tgccataccc caccataaag cgaatcatca agccacaagc cggggcaaga aacataaata
      361 tccccgtcac tagttcatta ccgccagtta accacatggt gaagtaataa atgagtgcag
      421 cgaggatcac catgccgaag aaagaagaga ccgccgatac cacgttcagt acctgaccga
      481 tgtagccgct tacgcagaca atacacaggg cgagaaacat ccataaataa atcatagctc
      541 aacctcctgc cgcgctaaaa aacctgtaac ttcagtcact ttaatagtaa gacaatcctt
      601 aacctctggc aatttttgct aagcatcaat gaaaacagca tgttaaatgc aagactgttg
      661 tgtacggaaa aatatttact ttgcacgatt aataatcagt agctgaaagc agtcagcgag
      721 tgataaagta cagataccgc attcatgctc ctcgtggtta tgtcctgact agtctttaca
      781 ctctttacag gaaccattgt cgtacatgat ggcccaacca attgagtgta ccgctgtcga
      841 catggaatct tctgcgatac aacaattcgt atctacagaa ggtaactatg tttccacaat
      901 gcaaattttc ccgcgagttt ctacatcctc gctactggct cacatggttt gggcttggtg
      961 tactctggct ttgggtacag cttccttatc ctgttctctg ctttctcggc acgcgtattg
     1021 gcgcaatggc gcgaccattc ctgaaacgtc gtgaatctat cgcccgtaaa aacctggaac
     1081 tttgtttccc gcagcattct gcggaagaac gcgagaagat gattgccgaa aactttcgtt
     1141 cactcggcat ggcgctggta gaaaccggca tggcatggtt ctggcccgac agtcgcgtac
     1201 gtaaatggtt tgatgttgaa gggttggata accttaaacg cgcacaaatg caaaatcgcg
     1261 gcgtaatggt tgtcggcgtc cattttatgt cgctggaact gggcggccgc gtgatgggac
     1321 tgtgccaacc aatgatggct acctatcgtc cacataataa tcagctgatg gaatgggtgc
     1381 agacccgtgg gcgcatgcgc tctaacaaag cgatgatcgg cagaaataat ctgcgcggca
     1441 ttgtcggtgc actgaagaaa ggtgaagcgg tatggtttgc tcccgatcag gattatggtc
     1501 gtaaaggcag ctccttcgcg ccgttctttg cggtggaaaa tgtcgccaca accaatggca
     1561 cctatgttct ctcccgtctc tctggcgcag ccatgttgac cgtaacgatg gtaagaaaag
     1621 cggattacag cggatatcgt ttgttcatca ccccagagat ggaaggctac ccgacagatg
     1681 aaaatcaagc cgctgcctat atgaacaaga ttatcgagaa agagatcatg cgcgcaccgg
     1741 agcagtacct ctggatccac cgtcgcttta aaacgcgccc ggtgggagaa tcgtcgttgt
     1801 acatttaaca aactgctctt actctttccc tcctcaaatc ggccataata acctcatggt
     1861 ttttatggct tttttcttaa ggttttttcc ttcgctgaat atctaattat taccgtcaga
     1921 aattaaactg tcgccgatat gagacacttg taagttacta aaagtaaata agaaaatgtc
     1981 tcttgtctcg tttcagtttt aggcgtacat tagcgccgtc tcgagcagtt tagctcagaa
     2041 ttctggggtg atgcccacgg taaaatacag ggcttttaag gtcgctttgc ccggtgtcaa
     2101 ctcactgtat caggtgtaat gaagtcattc aggcgtaaca gtaattacgc ggagagatgt
     2161 aaagtgaaat atttctttat gggcatttca tttatggtca tcgtttgggc cggtactttt
     2221 gccctgatga tctaaaagca aactgtgcaa tgaaaaacag gagccatcag gctcctgttt
     2281 gcatttctga cgacggtgtt tattccgcgt tttcgtgaat atgtttgctg ctggcgggta
     2341 gtaaaccgtc ggcacggaac atcgctttaa tcccacgaat cgcctgacga atacggtcgc
     2401 ggttttcaat cagtgcaaag cgaacatgcg tatcaccata gtcgccaaag ccaatccccg
     2461 gcgagacaca aaccttcgct tcgttaagga gcttcttggc aaattccagc gatcccatgg
     2521 ccgcatatgg ttccgggatt ttcgcccaga catacatcga agccttcggc atttcaacca
     2581 tccagcccgc ttcatgcagc cctttaacca atacatcacg gcggcgtttg tactgttcgg
     2641 caatgtcgcg cacgcactgt tgatcgccct ccagcgccgc aatcgccgcc acctgcaacg
     2701 gcgtaaaggt gccgtaatcg tgatagcttt taatacgagc cagggcgctg accagcgttt
     2761 tgttgccaac cataaagccg atacgccagc ccgccatgtt gtagcttttc gacagcgtaa
     2821 agaactcgac tgccacatcg cgtgcacccg gtacctgcat gattgacggc gctttccagc
     2881 catcgtagac gatatcggca taggccaggt cgtggaccac cagcacatcg tagcgtttcg
     2941 ccagcgccac aaccttttcg aagaactcca gctccacgca ttgcgcggtt ggattcgacg
     3001 ggaagccgag gatcatcatc ttcggtttcg gataactttc acgaatggcg cgctccagtt
     3061 cgttgaagaa atcgacacct tccaccagcg gcactgagcg tacctgcgcc ccggcaatca
     3121 ccgcgccata aatatggatc gggtaacttg gattcggcac cagcaccgtg tcaccatgat
     3181 ccagcgtcgc cagcatcaga tgcgccaggc cctctttcga accaatagtg acgatggctt
     3241 ctgattccgg gtcgatttca acgtcgtagc gatcctgata ccagcgggaa atggcgcgac
     3301 gtaaccgcgg aatgccgcgt gaagtggagt aaccatgcgt gtccgggcgc tgggccacag
     3361 tacataattt ttcgacgata tgcggcggag tcgcaccgtc cgggttaccc atgctgaaat
     3421 cgataatatc ttcgccgcgc cgacgcgcag ccattttcag ttcagcggtg atgttaaaaa
     3481 cgtagggcgg gagacgatca atgcgcgtaa agcgacgttc agggcgagtg tcagccataa
     3541 tttcctcaga ttaacgttag cgcccggacc gtccgagcga cgctgccaca atggtggctg
     3601 ttttgaaaat agcctgatta atttctgtct gtccagcgtt ttagaacatt tttatcaatg
     3661 gctttaaatg aggaactcca cttcaaaaac aggaaatttt tctacgctaa caagaaaatt
     3721 accaccaatt ttattaacat
//
LOCUS       DBANUU010000268         3709 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000268 DBANUU010000000
VERSION     DBANUU010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.273"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..453
                     /locus_tag="ACIE68_004718"
     CDS             <1..453
                     /locus_tag="ACIE68_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFX5910885.1"
                     /translation="AKHNRQDVLCCVSCGCPLTLQRGNDGQPPWFEHDQMTVAEKILL
                     RCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVMCDEDYEGEKCCPRCGTGVYSRE
                     GGCRKATGRIGTERFSRGNQLQSCNDISGSAALVGASRDREILALCPE"
     gene            628..2247
                     /locus_tag="ACIE68_004719"
                     /pseudo
     CDS             628..2247
                     /locus_tag="ACIE68_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            2327..2619
                     /locus_tag="ACIE68_004720"
                     /pseudo
     CDS             2327..2619
                     /locus_tag="ACIE68_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(2659..3510)
                     /locus_tag="ACIE68_004721"
                     /pseudo
     CDS             complement(2659..3510)
                     /locus_tag="ACIE68_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
     gene            3576..>3709
                     /locus_tag="ACIE68_004722"
     CDS             3576..>3709
                     /locus_tag="ACIE68_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFX5910886.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     "
BASE COUNT          931 a          876 c          998 g          904 t
ORIGIN      
        1 gcgaagcaca accgccagga cgttttgtgc tgtgtgtcct gtggatgccc gttaacactt
       61 cagcggggca atgacggaca accaccgtgg tttgaacatg accagatgac tgtcgctgag
      121 aaaatcctgc tgcgatgcac ctggcttgac ccggcagaga aagaggcccg tcgtttgcat
      181 ctgcagggta tgacggttcc ggattatacg gtgaaggtga gaaagtggtt ttgtgtgatg
      241 tgtgacgaag attatgaagg agaaaagtgc tgcccgcgtt gtggtaccgg ggtatacagc
      301 agggaggggg gctgcaggaa ggcaactgga aggataggaa ctgagcggtt cagtcgcgga
      361 aatcagttgc agagctgtaa tgatatctcc gggagtgctg cgttggtagg ggcatcccgg
      421 gatagagaaa ttttagcact ttgccctgaa tgattgtaga cgttccgagt actcgtaccg
      481 gtctgagtta ttacagttag atattcactt tttctcccgc atacttcaca tctgtcaacg
      541 acggatgaaa agtggttctt ccgcaacctg ggtggaagaa aatggatgaa actttctggc
      601 gaacaaggat ctaagctgtt ttaagttatg ggcaacgcaa tgcactctct taagacactt
      661 ctacagttac cttgcggatg gcgatgcagt cgacaaatta ttagctctga cggtataacc
      721 ctccatctcc acggaaaacg caaaacagca caatgtcctg aatgctttaa gcgtagcgac
      781 tctgttcata gttgtcgtcg gcgccggata cagcatctac cctgctcagg gcagacgcta
      841 tggcttgtat tttccgtccg ccactggtac tgccgtaacc cggcttgttc acgtaaaatt
      901 tttgccgagt cgcttgctcc cttcgccggt tcacaccagc agtcttcaca ggcgttacaa
      961 aatttacaac gtcaactggg attaatagct ggaggtgagg ctggaaaacg ggctgcaacg
     1021 gcagcgggtc tccgttgcag tgctgatact cttcttcgca gggttatcaa taccccggag
     1081 acgaaacagt caggcgcgcc tcatgtcggt attgatgagt gggcgtggca tcggggccac
     1141 tgctgcggta tgttaatagt caatcttgat actcaccgtc ccctcgtcct gcttcccggc
     1201 cgtgatcagc gtacgctggc gacctggttc agaaaatatc cggaaataca ggttgtctcg
     1261 cgtgatcgca gtggagtcta tgcgacagca gcacgtgaag gtgcacctca ggccagacag
     1321 gtggccgatc gatggcacct gctaaaaaat attggtgatg agcctgaacg aatgatgtac
     1381 agacatatgc ctctgatacg tcttgttgtc agagagttat cactgaagaa atcacctgag
     1441 ccagaaatat ctgtgcctgt agcatcgctc cgtcgtctgg aacgccttaa acagcacatc
     1501 cgcaaaaaac ggcatcagcg ttggacagag gttatggccc tgcataacaa gggatgtagt
     1561 ttcagggaaa tatcccgtat tacaggcctg tcgcgagtga cagtcagtcg ctgggtgggt
     1621 tcaggaacat tccctgaaat gtcaactagg cctccaaagc gagggcttct ggacccatgg
     1681 agggagtggt taaaagagca acgagaatgt ggtaattata actccggccg gatatggcgg
     1741 gaaatggtgg ccaggggggt tacaggcagt gaaaccatcg tcagggatgc tgttgccaaa
     1801 tggcgtaaag gctggatccc accggttact actgccgcaa gacttccttc agtgtcccgg
     1861 gtaagccgct ggttgatgcc ctggagaata atcaggggtg aagaaaatta tgctttccga
     1921 tttattagtc tgatgtgtga aaaagaaccg gagttgaaaa tagcgcagca actggtactc
     1981 gagttctatc gtattctgaa aacctaaaat aaatcacagc taagcagctg gttcactcgg
     2041 gtccacgaaa gcggctcagc agaacttcgg tgcgtagctg cggggatgga agctgatgct
     2101 gcggctatat gtgaggcaat cagcagtcgc tggagtactg gtgttgtcga aggtcatgta
     2161 aatcgcctga aggtgctgat acgccagatg tatggtcgag ccggacttga actacttagg
     2221 cggagggtaa tgagtccact ggcatgaaaa tcttcaccca ggttgcggaa gagccacttt
     2281 tgcactgttc gtgacagtat ggcctttatg tactcacttt gttaccggta gctggatcat
     2341 cactaccgtg ctgcaaatgg tggtgaacag catcaattgt ggaatgaatg ttgccgaagc
     2401 gaccaatgcg cctcgtttcc actatcagtg gttgccggac gagctgcgtg ttgaaaaggg
     2461 tttagtccgg atacgctcaa gctgctggat gcaaaaggtc agaaagtggc gctgaatgag
     2521 gcgatgggca gcacgcaaag cattatggtt gggtcggatg gtgagttgta cggcgcatcc
     2581 gactcgcgct tggtggatga tttaacggcg gggtattaat tgacatcccc cggcgcagtg
     2641 tgccggggga taatggtttt atgcggtcaa taatcgtccg ggattatcga tacagagttt
     2701 tttgaggatc gttttatcga cgccgcgctc cgccagatag gccagaaaaa catctggaac
     2761 aaatccccag ccattaccgc catttttagc ccacatttgt ttaaggaaga catcgtggct
     2821 gagaacaagc tgatcggcgt atcccaactc aatgagatga gcgacggcat cggcagtctc
     2881 ctgcacccca ggcgctattc cctctttcgg gaaggtaatg tctaggccaa tcatgtcgaa
     2941 ttccagccag acacccttat caagcatttt ccgctgatac gccacgtctt ttcctgacgg
     3001 atccgagtgt gcgagagaga ctttatttgg cgagacgccc atttcaccta acacaatgtc
     3061 gagtacttca tcaccgcgac gaagccagcc cggcatgtga atattcatcg ccacatgagg
     3121 attgttaatc tgtgccagcg aggcagcccg caagctgtta tgctcggctt cggtaaatgt
     3181 cggtgagaca cctatttcac cgatcattcc ggcacgaata tccgtatcgc caatcccttg
     3241 attcaattct ttatcaatcg ttgccgccag ttcatcaacc gttttatgaa ttctctggct
     3301 ttcaaatttt tccaggtagg gaccggagga agcaacaata ttcagacctg tttttaatgc
     3361 gacttctcgt aatgcctgcg catctcgtcc gatagactca gaccctgtgg catcaacaat
     3421 ggtgcgacca ccgagcgaga taaagttatt aatttcgaag atcacatctt caataggttt
     3481 gcggtccata ttatcagcgc agcaataagg tgatcttacc cagcaatagt ggacacgcgg
     3541 ctaagtgagt aaactctcag tcagaggtga ctcacatgac aaaaacagta tcaaccagta
     3601 aaaaaccccg taaacagcat tcgcctgaat ttcgcagtga agccctgaag cttgctgaac
     3661 gcatcggtgt tactgccgca gcccgtgaac tcagcctgta tgaatcaca
//
LOCUS       DBANUU010000269         3606 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000269 DBANUU010000000
VERSION     DBANUU010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.274"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1958)
                     /gene="traD"
                     /locus_tag="ACIE68_004723"
     CDS             complement(<1..1958)
                     /gene="traD"
                     /locus_tag="ACIE68_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFX5910887.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PQQPQQPQQPQQPVSPAINDKKSDSGVNV"
     gene            complement(2009..2746)
                     /locus_tag="ACIE68_004724"
     CDS             complement(2009..2746)
                     /locus_tag="ACIE68_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910888.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSCRIDGHQAENITWAISSQS
                     PADKYDLINKTSRVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRAAGGYHHRHTGYTWSVSVN"
     gene            complement(2949..>3606)
                     /gene="traT"
                     /locus_tag="ACIE68_004725"
     CDS             complement(2949..>3606)
                     /gene="traT"
                     /locus_tag="ACIE68_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFX5910889.1"
                     /translation="TAIKKRNLEVKTQMSETIWLKPASERTVFLQIKNTSDKDMSGLQ
                     GKIADAVKAKGYQVVTSPDAAYYWIQANVLKADKMDLRESQGWLNRGYEGAAVGAALG
                     AGITGYNSNSAGATLGVGLAAGLVGMAADAMVEDVNYTMITDVQIAERTKATVTTDNV
                     AALRQGTSGAKIQTSTETGNQHKYQTRVVSNANKVNLKFEEAKPVLEDQLAKSIANIL
                     "
BASE COUNT          890 a          962 c          860 g          894 t
ORIGIN      
        1 acattcacac ctgaatctga cttcttatcg tttatggcag gagacaccgg ctgttgcggc
       61 tgttgcggct gttgcggctg ttgcggctgt tgcggctgtt cagcctgagt cacgtcttct
      121 ccggaaacaa cttccggcac atccggttcg aagaggctgg ccatctgacg accttctgct
      181 tcccttgcgg caagtacggc actcagacgg ttctccattt ctgggttgat gtcacgcgga
      241 ataaactcag gggcaacctt cggacgtgcc tgatatttca gtgagagttt tactgccgga
      301 tacggtccgg gcagggtgac ataacaggtc agatccggca gagactgaat gtcggaataa
      361 ctgaccagcg tctggcgctc catatcctta ccggtcgata ccccgtcacg taccggatca
      421 gcaccgtagg aatactgctc gctggctttc aggtgctctt tctcaccaat ctcaccggca
      481 gcgaactctg caatcttatg gctgggagaa cggaaaaagg cacgggtgtt catgacgtca
      541 aacagcgtgg cagccgcttt ctcaccgtag atatcttcca gctgggcata ggactggata
      601 ccaaacacat aacagccacc gaacttacgg gcttccggca ggatctccac caggtccggc
      661 agtttgtgta acgtgggtaa ctcgtcacag aaaaaccaca cacgacggtt acgattttct
      721 cccattgcca gcagaccacg aatggcaatg gacagccaca tggagatcac cggtttcagg
      781 gaggcatggg tgtcggcatt cgacgaaata aacagccagc cgtttttctg atcttcccgg
      841 acaccccgca tccagtcacg gatggtgaag ggctcaccgt tatgctcaat cccctgcagg
      901 taacggatag ctttcacgta gttggtcagc acagcacgga tggaaattgc cgttttctca
      961 attttctctt ccaccaggtt ggccgccggt gaattacgca ggaaggtacg cagtttttca
     1021 attttgatgg aaagcagtgt gtccaccagt ttgctgtagc tgcggttggg gtcattacgc
     1081 atcaggtacg ccgcttccgc aaaaatggta cgtcctgaac cctgccagaa cgggtcttct
     1141 tttgttccca tagggatcag agtatttgcg gtattatcaa aatccggctg tgtcaggcac
     1201 tccttccaca aatcccatgc ggcacagcgg gcatccagcg gattcaggat tttatcgatg
     1261 gagggatcat agtaactttt aacaaattcc cctgaacggt cataaatcac caccatatca
     1321 ccgcgctgac gggcgtagtt ggccagacga cggataacct ccgacttacc ggccccgacc
     1381 gtgccgtgca gacagaagtt ctggatttcg gaatcccgga taatcggcag gtcgccaatc
     1441 cggatatcgg agtccttgcc gtcttttttc agcatccggg caacgtcttt cggattgtct
     1501 gtcagctgac gaccacctgt gacttcattt tcgctctgtt gtttaccctg acgccccaga
     1561 atccaggaga caacaaagaa ggtgatcagg caaataacca gggccacgac agacgccaga
     1621 acgaatgcag accatagctg ctcaccgcac cagatcatgt atttatcatg cagtacctga
     1681 gccgcgttca tccggaaggt tttgccgtaa tactggatct catatactgg ctgggacttg
     1741 attaaatccc gcatgccttc cagcgtggta caccaccagt aaatacagcc gttcacaaac
     1801 gtctgccagc ttatttttac ccataaaacc agaccaacga gtatccagaa aaaaataaac
     1861 aggcagtaaa gcatgatatt ggcgatctgg ctgaacatgc ggatacgcat ggacgcaatc
     1921 tgaccgccct gggtcatatc ctttgcgtta aaactcatga tgaaactccg ggaaaatctg
     1981 gaacagggca gaaaaggatg atgtggggtt aattcacaga aacagaccag gtgtaccctg
     2041 tatgccggtg gtgatacccg cctgcagccc tgccggaaga gaccttctct gtaacaatat
     2101 cagtcaggcg caatacttca gaccagtcac tcccgtgcgg aaactggatc tgaataaaaa
     2161 ggcgtacccg ttcacggtga tccagaactg tgtccggttc ataaaaacgg ccactgtcag
     2221 gagaaacacg ggacgtttta ttgatgagat catacttatc ggcaggagac tgcgatgaaa
     2281 tcgcccaggt gatattctcc gcctggtggc catcaatacg acagctcccc ggcgtccatg
     2341 caatttcctg cgcactcgcc acgatgatct ccctggagac ttctgccagc tttctgggtg
     2401 tacagaatac cggtgccagc acacacaggg acaacaaatt acagctgacc gcctccggtt
     2461 taagcaaatt gctcccggat ttttcaggaa aagatatttt tgtcagtatt tcagttaatt
     2521 ccttatgcct gagagaatta ataaagtaca tgataagcat catgagaaac aatataacaa
     2581 taagccctcc gataaccact aaaaggtcag tatcaatatt attgctgtac agatagtctt
     2641 tagtggtcac tgtattatcc tgtatcaccg gggggtattc agttacaggc ttctcagtaa
     2701 attgattatt ctcaccaaca tattcagcga tattgtctga tgtcataatg actttctccg
     2761 gaagatcccg gcttcgccgg caacacttca cggtcgcaca aagaatttgc acaaccgctc
     2821 agtgttttca tatttatttc ggaaacgccg ggaggtgaca aaatttattt tgttacatcc
     2881 cggggttgga caacgagcaa cgcgaggcgt cagcaaggag gccggtcaga tcagcctcct
     2941 gacgaggttc agagaatatt tgcgattgat ttggccagct ggtcttcgag aacaggcttc
     3001 gcctcttcaa atttcaggtt aaccttgttc gcatttgaga ccacacgggt ctggtattta
     3061 tgctggttac ctgtttcagt actggtctga attttcgcac cggatgtgcc ctgacgcagg
     3121 gcggcaacat tatccgttgt caccgttgcc ttagtacgct ctgcaatctg cacatccgtg
     3181 atcatggtat agttcacatc ttccaccatc gcatctgcag ccatacccac cagaccagca
     3241 gcaaggccta caccgagtgt ggcaccggca gaatttgagt tatagccggt aataccggca
     3301 cctaacgctg caccaacagc tgcgccttca taaccacggt tcagccatcc ctgagactcc
     3361 cgcagatcca tcttatcggc cttcagcaca ttcgcctgaa tccagtagta ggctgcatcc
     3421 ggagaagtca ccacctgata tccttttgct ttcacagcat cagcaatttt gccctgcagc
     3481 ccactcatgt ctttatcaga cgtgtttttg atctgcagaa ataccgtgcg ttcgctggcg
     3541 ggtttaagcc agatggtctc actcatctga gtcttcacct caaggttacg cttcttgatt
     3601 gctgtg
//
LOCUS       DBANUU010000270         3537 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000270 DBANUU010000000
VERSION     DBANUU010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.275"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(495..1025)
                     /locus_tag="ACIE68_004726"
     CDS             complement(495..1025)
                     /locus_tag="ACIE68_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFX5910890.1"
                     /translation="MKLTEESLNLVIDILNDLICEGKMLNDVERRSLLNAVMAITAVK
                     ERSVVSVSSRKEQSKKEKKEREIDPRFPNAGVAWNEADESLLRDVLEDVPDDEIGKHL
                     FWLAEKLGRTPYSVACKVRQIKKLPAEWKDQFRKISDRIRSSGMSVSEYLQRQGLLSP
                     ESGEENNPVSHNNNVA"
     gene            1695..2426
                     /locus_tag="ACIE68_004727"
     CDS             1695..2426
                     /locus_tag="ACIE68_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFX5910891.1"
                     /translation="MNQPIHNDYWLSRFESILNSALVQHRAVSLIRVDLRFPEHMPVT
                     IMDPDPDSAVISRFFESLKAKIQAYQRKKRRTNKRVRATTLHYFWCREFGKEKGRKHY
                     HVILLLNKDTWCSPGDFTVPSSLATLIQLAWCSALHLEPWQGNGLVHFSRRTPFRKPA
                     SSDARPSSDDTPLSGGCSETRKASDKKPGEAAVLWIKRGDVEAMQKAMERARYLVKYE
                     TKQHDGSGQRNYGCSRGAGRLLDGR"
     gene            complement(2488..2994)
                     /locus_tag="ACIE68_004728"
     CDS             complement(2488..2994)
                     /locus_tag="ACIE68_004728"
                     /inference="COORDINATES: protein motif:HMM:NF017673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFX5910892.1"
                     /translation="MMKILNQSLKSILPPPKIKPSEWCEQYTVFPDGPLAQQKTKLFS
                     FQVEPLNATVDPSVQKICLMSSAQLLKTTILQNAMLYRMANKPSHMIFAGSSGSTVKK
                     FRTGKWMDTINACPNLQALITDKSDKNAVNDLHTQQNVDGIAATNDGCMKTRKHCMTS
                     GRLFQKTS"
     gene            complement(2939..>3537)
                     /locus_tag="ACIE68_004729"
     CDS             complement(2939..>3537)
                     /locus_tag="ACIE68_004729"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910893.1"
                     /translation="GSFAANYFFCILNILNNTNKTWINKLNDRYTTNEIAERMGVNDS
                     TVSREWAKRGMPHPKKASKKEIDQWILINIIQPLRNTDVKEQIEKEKLRKISLEADLV
                     EIELNISTEKVLSTDYVTQTLTGYFAALRGVLRSIPASIYVELFESEDADTLRIKLEE
                     RIDEKLRELGSYEYEEQPEEDDEDSEPESEINITPTED"
BASE COUNT          882 a          734 c          742 g         1179 t
ORIGIN      
        1 taactaaatc agtttaatgc tgctgtaaaa cctttctgtt taagcaatca atctgctatt
       61 cgattttact gatgttacgt ttaacctttc cttagatttc gagtttcgac gttagcttga
      121 gaaaagtatc tttacgtaaa gcccagtgaa ttaactcaaa tccgattgag gtaacctgat
      181 gcgatttact gtgagtttgc gataacactg ttaaatggct tatggatgat tttgtaacgg
      241 cgagattgat aaggtgatta actggccctg gtgttttttc tcagcgtaag gtgattatct
      301 tcatcataag gctcttttca gaaaaagtgt gatgattggt tgtggtgttt tgattcagat
      361 gaggatctgc caatgattat gatgtattgt aagaaataac gccttcagaa tgttggagca
      421 tgcacttaca caaactgttt ttctaaaatt aagggaacag tttaaagagt ataaactgtt
      481 catcttcttc aggattaagc tacattgttg ttatgactga caggattgtt ttcttcccct
      541 gactctgggc ttaacagtcc ctggcgttgt agatattcac tgactgacat tccagaacta
      601 cggatcctgt cagagatttt acgaaattga tccttccatt ctgcaggtaa tttcttaatt
      661 tgtctgactt tacaggctac actgtacggg gttcgcccta gtttctctgc cagccagaaa
      721 aggtgtttac caatttcatc gtccggtaca tcctccagaa catctcgcaa taaactctcg
      781 tctgcctcat tccatgccac accagcattt gggaaacgag gatcgatctc tctttccttc
      841 ttctccttct tactttgctc ttttcgggat gatacagata cgacagaacg ttcttttacc
      901 gctgtaattg ccataactgc attgagcaga gatctgcgct ccacatcgtt cagcattttt
      961 ccttcacaga tcaaatcatt caggatgtca atgactagat tcagactttc ttctgttagc
     1021 ttcatatttc agaccttgaa gtatgtagat aatcagcaca attactaatg tgataaatat
     1081 cagaagataa tttacaggta aaccggaaaa tacatctgaa gaataaaggc ctcagcttaa
     1141 cgtttcagcc agtttgtgag ctgattgagg tacggcgatg acattaacgg gaattactcc
     1201 cctatagctc tgagcttatt tttcaccctg gcaacatatg gtggctactg cgcatggttt
     1261 tggagtagat atcttactac tcgtagaatt gtgcttactg gtcaggccag cgcacaggca
     1321 ttccgtgcaa tcaatagaac actggttttt tagtcttccg ttacccatca ggatgttagt
     1381 gcagattccg gtgtattcga tcagttgttc ggcgaatcag cgatcgatca cgatgcgatt
     1441 tcgtatgtta gggatgctgg tatgattact cgctgaaaaa taatgtgaaa aggcagtttt
     1501 tctttagaca tttagctcat tcatgctgtt gttttacgtt ttgctgtcgt gtgcaggatt
     1561 atcttttcgt tacgggacga ttcattccgt tttaatcagg agctattggc gttgctcatt
     1621 ggtgggatgc cgtaaagttt taccgcggcg attaatgatg tgaagtcaat ccaaatcaac
     1681 ggagatctct catcatgaat caaccaatac acaatgatta ctggttatcc cgttttgaaa
     1741 gtattctcaa cagtgccctg gtgcaacacc gtgccgtctc gttaatccgg gtggatttac
     1801 gtttccctga gcatatgcct gtcaccatca tggatcccga tccggattca gcggtgattt
     1861 ctcgtttttt cgaatccctg aaagccaaaa ttcaggctta ccagcggaaa aaacgacgta
     1921 ccaacaagcg tgtgcgtgca accaccctgc attatttctg gtgtcgggag tttggcaagg
     1981 aaaaaggcag gaaacattat cacgtgatat tactgctcaa caaagatacc tggtgctcgc
     2041 caggggattt caccgttcct tcttcgctgg cgacgctgat ccaactggca tggtgtagcg
     2101 ctctgcatct tgagccctgg cagggtaatg gactggttca tttttccagg cggacgcctt
     2161 tccgtaaacc ggcatcatct gatgctcgcc cttcttccga tgatacgcct ttgtcgggtg
     2221 gatgttctga aaccaggaag gcttcagaca aaaagccggg tgaagccgct gttctctgga
     2281 tcaagcgtgg tgatgtggaa gcgatgcaga aagccatgga gagagcccgt tatctcgtga
     2341 agtatgagac gaagcagcat gacggttctg gtcaacgtaa ttatggttgc agccgtggag
     2401 cggggcgtct actggatggc aggtgaaccc tgtaaaacgg catccggtgc cagagtatat
     2461 gtcacagtaa gggcgtggtt gacgccctta gctcgttttc tgaaaaagtc gtcctgaagt
     2521 catgcaatgt ttcctggttt tcatgcatcc atcatttgtc gctgcgatgc catcaacgtt
     2581 ctgttgagtg tgtagatcgt ttactgcatt cttatctgat ttgtcagtga ttaatgcttg
     2641 aaggtttgga cacgcattaa tagtatccat ccatttacca gtacggaact ttttcacagt
     2701 actaccacta ctacccgcaa atatcatatg ggagggttta tttgccatcc tgtaaagcat
     2761 tgcattttgt agtattgttg tcttgagtaa ttgtgcacta ctcattaaac agatcttctg
     2821 tactgatgga tctactgtgg cgtttagggg ttctacttga aatgaaaata atttagtttt
     2881 ttgttgtgcc agtggtccat ctggaaatac agtatattgt tcacaccatt cagatggttt
     2941 aatcttcggt gggggtaata ttgatttcag actctggttc agaatcttca tcatcttctt
     3001 ctggctgttc ttcgtactca tatgatccta attcccttag tttctcatca atcctttctt
     3061 ctagttttat ccgcaatgta tccgcatctt cactttcaaa tagttctaca tagatagatg
     3121 ccggaatact tcttagtact ccgcgtagtg ctgcaaaata accagttagt gtttgagtta
     3181 cataatcagt actcaatacc ttttcagtac tgatatttaa ttcaatctca actaaatctg
     3241 cttcaagtga tatcttgcgt aatttctctt tttcaatctg ttcttttaca tcagtatttc
     3301 gcaatggttg gataatatta attagtatcc attgatctat ttctttttta cttgctttct
     3361 ttgggtgtgg cattcctcgt tttgcccatt cacggcttac agtactgtca ttaactccca
     3421 tacgttctgc aatttcgttt gttgtatatc tatcgttcaa tttgtttatc caggttttgt
     3481 ttgtgttatt taaaatattt aatatgcaaa aaaaatagtt cgcggcgaaa cttcccg
//
LOCUS       DBANUU010000271         3516 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000271 DBANUU010000000
VERSION     DBANUU010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.276"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            28..1374
                     /gene="yagA"
                     /locus_tag="ACIE68_004730"
     CDS             28..1374
                     /gene="yagA"
                     /locus_tag="ACIE68_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YagA"
                     /protein_id="HFX5910894.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKRPATMAQHNLA
                     NDLLREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSFGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGESSSVRKKRPRLA
                     RLLLSLIDDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRSLSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNEHPPFKKHMYGVFMHLRY"
     gene            1653..>3516
                     /locus_tag="ACIE68_004731"
     CDS             1653..>3516
                     /locus_tag="ACIE68_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Ia central receptor-binding
                     domain-containing protein"
                     /protein_id="HFX5910895.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSAQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLNTARNALTRAEQ
                     QLTQQKNTPDGKTIASPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAES
                     AVNSARNNLSARTNEQKHANDALNALLKEKENIRNQLAGINQKIAEEKRKQDELKATK
                     DAINFTTEFLKSVSEKYGAKAEQLAREMAGQAKGKKIRNVEEALKTYEKYRADINKKI
                     NAKDRAAIAAALESVKLSDISSNLNRFSRGLGYAGKFTSLADWITEFGKAVRTENWRP
                     LFVKTETIIAGNAATALVALVFSILTGSALGIIGYGLLMAVTGALIDESLVEKAN"
BASE COUNT         1026 a          764 c          884 g          842 t
ORIGIN      
        1 agcatgtaca taaggagtgc tttcattatg ccaatgatac ttgtaaagaa gaatccccgg
       61 ggcaaagtga ttcgggagct tagcagtgag gaagagactg cagtaaagac ggtctgtgga
      121 ctgaaaaggc cagcaaccat ggcacaacat aacctggcca atgatcttct ccgggaaatg
      181 cgtgagtatg atgcatggtt acagtgtgac tgtattccgg gagattcgcc ggccatgaat
      241 tttgctgctc ttaaaaacaa tacgggtact ctttatctgt ccagttttaa tcatgagcat
      301 gcacctgaat gccccatgta tcgtcagtta tccggaaatg aagaggaaac atccttcgga
      361 gcaagccggc atcccgttag cacccgtata aattacagaa actttttacc acctgatgac
      421 agtaattctg ttatcagact gcaggcccgc tctgcttacc ataatggtga gagctcctcc
      481 gttcgcaaga aacgtccgcg ccttgcccgt ttactcctgt ctcttattga cgatgctggg
      541 ctgaataaac tggattccct ggctaatccc cgtatccgga ctaattatcg tgagtgtctt
      601 gatgccatac ggcaggttac actgcagcag gagtatattc ggggaagatc cttatctgaa
      661 attatccatt tccggcctgg aatgtctgaa aggtctcagg agcggttaat ggaaacgctg
      721 gagaattcag aacgacactg gcctgccaga agaaagcata tgttctttca gatttttatg
      781 gcgcagcaca tctgtcgtga tgctgtggaa attcactggg cgaacggtaa tattcaggtc
      841 atcagacctg tgcgggggat cagtattaat ggtgaagcgc agggcggaat acgtccccct
      901 tactgggtta ttcttgcttt ttgccggagt gccgatggca gaatcatctg cagtgaaggg
      961 tatgctcatg ctctttatca actgacatgt ccggtgcctg tggacagcaa actggaacga
     1021 aacacgctca ctgctcttct gaatgtggcc agctggctta aaagaaagcc aggtacgccg
     1081 gaattaagtc tggaaaggcc cctgtttgat acagaagttt atgttaatgg tgaaaagaaa
     1141 tatgtactgc cggatttcat tgtcacagca agggctcctg acggaaagac ggccagagtg
     1201 gtcatcgaaa cgatgggata tgaagacagt gattactgcg cgagaaaatc caggcagcat
     1261 accggcatga agcagattgg tgttctgcat accgatccac cgaaatggct ggataacgag
     1321 catccccctt ttaagaaaca tatgtacggt gtttttatgc atctcaggta ctgagatatt
     1381 ttgtggctca gttctgtaac ttttcccgta acattgtctg ttgttacggg aaagtccggt
     1441 ttttgtattg caccagagaa tacccagact gtgatgctgc cacagcgtca gcaggctttc
     1501 tgaacggtgt aacatcactt catgttaatg ataatcacta tcatcaaatc ttgacatgcc
     1561 attttctcct taataaatta atactgtata tgtatccata tgcgtaagca gttaattcat
     1621 ttgttttcct cagaggatga aggagatacc gaatgtctga ccctgtacgt attacaaatc
     1681 ccggtgcaga atcgctgggg tatgattcag atggccatga aattatggcc gttgatattt
     1741 atgtaaaccc tccacgtgtc gatgtctttc atggtacccc gcctgcatgg agttccttcg
     1801 ggaacaaaac catctggggc ggaaacgagt gggttgatga ttccccaacc cgaagtgata
     1861 tcgaaaaaag ggacaaggaa atcacagcgt acaaaaacac gctcagcgcg cagcagaaag
     1921 agaatgagaa taagcgtact gaagccggaa aacgcctctc tgcggcgatt gctgcaaggg
     1981 aaaaagatga aaacacactg aaaacactcc gtgccggaaa cgcagatgcc gctgatatta
     2041 cacgacagga gttcagactc ctgcaggcag agctgagaga atacggattc cgtactgaaa
     2101 tcgccggata tgatgccctc cggctgcata cagagagccg gatgctcttt gctgatgctg
     2161 attctcttcg tatatctccc cgggaggcca ggtcgttaat cgaacaggct gaaaaacggc
     2221 agaaggatgc gcagaacgca gacaagaagg ccgctgatat gcttgctgaa tacgagcgca
     2281 gaaaaggtat tctggacacg cggttgtcag agctggaaaa aaatggcggg gcagcccttg
     2341 ccgttcttga tgcacaacag gcccgtctgc tcgggcagca gacacggaat gacagggcca
     2401 tttcagaggc ccggaataaa ctcagttcag tgacggaatc gcttaacacg gcccgtaatg
     2461 cattaaccag agctgaacaa cagctgacgc aacagaaaaa cacgcctgac ggcaaaacga
     2521 tagcttcccc tgaaaaattc ccggggcgtt catcaacaaa tcattctatt gttgtgagcg
     2581 gtgatccgag atttgccggt acgataaaaa tcacaaccag cgcagtcatc gataaccgtg
     2641 caaacctgaa ttatcttctg acccattccg gtctggacta taaacgcaat attctgaatg
     2701 accggaatcc ggtggtgaca gaggatgtgg aaggtgacaa gaaaatttat aatgctgaag
     2761 ttgctgaatg ggataagtta cggcaacgat tgcttgatgc cagaaataaa atcacctctg
     2821 ctgaatctgc ggtaaattcg gcgagaaata acctcagtgc cagaacaaat gagcaaaagc
     2881 atgcaaatga cgctcttaat gccctgttga aggaaaaaga gaatatccgt aaccagcttg
     2941 ccggcatcaa tcagaagata gcggaagaga aaagaaaaca ggatgaactg aaggcaacga
     3001 aagacgcaat taatttcaca acagagttcc tgaaatcagt ttcagaaaaa tatggtgcaa
     3061 aagctgagca gttagccaga gagatggccg ggcaggctaa agggaagaaa atacgtaatg
     3121 ttgaagaggc attaaaaacg tatgaaaagt accgggctga cattaacaaa aaaattaatg
     3181 caaaagatcg tgcagcgatt gccgcagccc ttgagtctgt gaagctgtct gatatatcgt
     3241 ctaatctgaa cagattcagt cggggactgg gatatgcagg aaaatttaca agtcttgctg
     3301 actggatcac tgagtttggt aaggctgtcc ggacagagaa ctggcgtcct ctttttgtta
     3361 aaacagaaac catcatagca ggcaatgccg caacggctct tgtggcactg gtcttcagta
     3421 ttcttaccgg aagcgcttta ggcattatcg ggtatggttt actgatggct gtcaccggtg
     3481 cgctgattga tgaatcgctt gtggaaaaag cgaata
//
LOCUS       DBANUU010000272         3469 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000272 DBANUU010000000
VERSION     DBANUU010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.277"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            1..193
                     /locus_tag="ACIE68_004732"
                     /pseudo
     CDS             1..193
                     /locus_tag="ACIE68_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(236..1449)
                     /locus_tag="ACIE68_004733"
     CDS             complement(join(236..1158,1158..1449))
                     /locus_tag="ACIE68_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFX5910896.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQSEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQASAYFGEGGV
                     RPPLEKMMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLK
                     KEIQRVYDENHKVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTIS
                     RKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWRGFVYVAFIIDVFAGYIVGWRVSSS
                     METTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKA
                     YYASIGNDDLAA"
     misc_feature    complement(1052..1168)
                     /locus_tag="ACIE68_004733"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            1502..2344
                     /locus_tag="ACIE68_004734"
                     /pseudo
     CDS             1502..2344
                     /locus_tag="ACIE68_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2361..2670
                     /locus_tag="ACIE68_004735"
                     /pseudo
     CDS             2361..2670
                     /locus_tag="ACIE68_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(3134..3367)
                     /gene="papI"
                     /locus_tag="ACIE68_004736"
     CDS             complement(3134..3367)
                     /gene="papI"
                     /locus_tag="ACIE68_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major pilus subunit operon transcriptional
                     regulator PapI"
                     /protein_id="HFX5910897.1"
                     /translation="MSEYMKNEILEFLNRHNGGKTAEIAEALAVTDYQARYYLLLLEK
                     EGVVQRSPLRRGMATYWFLKGEMQAGQNCSSTT"
BASE COUNT          865 a          880 c          828 g          896 t
ORIGIN      
        1 ccgcgctctt gatgtcagac tccctgaaca gttctcgata atcgggaaac tcagagcgca
       61 ttatcctgtg gtcacactct gccatgtgtt cggggttcat cgcagcagct acagatactg
      121 gaaaaaccgt cctgaaaaac cagactgcag acgggctgta ttacgcagtc aggtacttga
      181 gctacatggc atctgaaccg ccccgggttt cctggagagt gttttatctg tgaactcagg
      241 ctgccagatc atcgtttccg atggaagcat aataagcttt ttctgcttct gccggaggag
      301 tatggcccag cctttccagc aatcgtcgat tgttatacca gtccacccac gtgagtgtgg
      361 ccagttccac ttctgcacgg tttttccagc tcttacggtg tattacctcc gctttgtaaa
      421 gaccattgat gctctccgcc atcgcgttgt catacgagtc gcctgtactt cctgttgatg
      481 ccagtaatcc ggcttcctta agccgctgtg tgtaggccag cgatacatac tgagaacctt
      541 tatcactgtg atggaccgtg ccggacggtc gacgggccca taacgcctgc tccagtgcat
      601 ccagcacgaa tgtcgtttcc atggacgatg agacccgcca ccccacgatg tatccggcaa
      661 acacatcaat gatgaacgcc acatagacga agccccgcca tgtgctgacg taagtaaaat
      721 cagccaccca cagctggtca ggtcgttctg ccacgaactg acggtttacg cggtcgcctg
      781 cggcaacggc tttccggctg atggtcgtac ggaccttttt accccggaga acaccggcaa
      841 gtcccataac cgccatgaga cgtgccacag tgcatctggc cactctgata ccttcccgta
      901 acaactgacg ccagacttta cgcacaccgt ataccttgtg attttcatcg tatacgcgct
      961 gtatctcttt cttcagccag tcatcgcgct gcgcacgggc actgcgttta tccggatgat
     1021 gtcgctgttg ctgacagtgg taatacgttg acggggcaat atgcagttcg ctgcatagcg
     1081 gtccgacccc gtactgctca cgcagcttat ccagcagtgg catcattttt tccagaggcg
     1141 gtcgaactcc gccttcgcaa aataagcgga agcctggcga aggatatcgt tactgcggcg
     1201 cagttcacga ttttcacgtt ccagctcttt cagacgctga cgttcagcgg tggtgagccc
     1261 tccatcaccg cccccggtat cccgctcatg ctggcgaacc cagacacgca gagtctccgg
     1321 cgtacagcca atctttggag caatggaaca aattgtcgcc cattgtgagt catattcgct
     1381 ctgactttcc agaaccatac ggactgcccg ttgacggact tcgggggaaa aacgagtatt
     1441 tttagtcatc ctgtttacct ctttctcagg aagtttagtc tccaggattc ccggggcggt
     1501 tcacgccggg ttagaagttc ttgaggtgac tgctccagac cggatggagc gacgcaaacg
     1561 gggtaaaagt gacacaattg atgctgaatg tgccgctcat gcagcattct caggcatcag
     1621 gacagttact cccaaaacgc gtgatggcat gattgagtcc ctgcgggtat taaaaacttg
     1681 ccgaaaaaca gcaatatcag cccgcagagt cgctctccag attatccatt caaatattat
     1741 ctctgccccg gatgaattac gtgaacagct cagaaatatg acgcgcatgc agctcatcag
     1801 gcccctgggt tcctggagac ctgatgccag tgaataccgc aatgtaacca acgtttatcg
     1861 catttcatta aagtcccttg cccgacgcta tctcgagtta catgacgaaa tcgctgatct
     1921 ggatgtcatg attgcggcaa ttgtcgacga gttggcacct gagctgatta aacgtaatgc
     1981 tatcggatac gaaagcgctt cacagttgct gatcactgcc ggagacaatc ctcaacggtt
     2041 aagatcagaa tcaggatttg cggcactgtg tggtgtcagc ccggtccccg tttcttctgg
     2101 aaaaacgaac cgttaccgac ttaatcgtgg tggagatcgt gctgcaaata gtgcacttca
     2161 catcatcgcc atcggacgct tacgaactga cgcaaaaaca aaggaatatg tcgccaggcg
     2221 tgtagccgaa ggacatacaa aaatggaagc gatacgctgt ctaaagcgat atatctcccg
     2281 cgaagtttac acgttactgc gcaatcaaaa caggcggatc aacagtatcc cgataacagc
     2341 ttgactctta gaagggcgtc cagggcagtc attatacgag caggcagttc cggcagttat
     2401 tgtggcgata ccagatcagt cagagtatga gccggcgcgg aaattgctgg gataacagcc
     2461 caatggaacg cttcttcaga agtctgaaga acgaatggat accggtggtg ggttacgtaa
     2521 gcttcagcga ggcagctcac gccataacgg attatatcgt tggatattac agcgcactaa
     2581 gaccgcacga atataacggt gggttacccc caaacgaatc ggaaaatcga tactggaaaa
     2641 aactctaact cggtggccag tttttgttga ccacttcaga tgcggacggt caaagatcgt
     2701 ggagttggat tttataaatc tgtctccagt agtagatgaa ctgaatacaa agttgccaga
     2761 gagtggtctg tcaggcaggg aatcagaaat tggatattat tgactggtac attgtgtcgg
     2821 gctgtgtacg ttctccccgc agacagtctc agttcgtaga atctataaat tactgctact
     2881 gatgctgccg gggaaaggcg taacgaaaaa acagcctccg ttaccggaca gcaaggaggc
     2941 tgaatggagt ttacaggatt tgctttttta taatgtctgg ccatgcagta aaaccggaca
     3001 ggttttatta tcatgtgagg tattctgaca taaaatgctg gatttttatt ttgtgacgaa
     3061 tgctgcaaaa ttgaatctgc actctgatgt aacttttatc tgtttcagtg aagcatgccc
     3121 acaaattgag ttattaagtt gtggaagaac agttttgtcc cgcctgcatc tctcctttca
     3181 aaaaccagta tgttgccatg cctcgcctta atggagagcg ctgaaccaca ccttcttttt
     3241 ccagtaataa caggtaatag cgggcctggt aatccgttac cgccagcgcc tctgcaattt
     3301 ctgccgtttt ccctccatta tgcctgttca gaaattccag tatttcattc ttcatatatt
     3361 cactcatctc actgtaacaa agtttcttcg aataataaaa atcatgcttt ctgttatcaa
     3421 cggaaaggta tttttattct ctgtgtttgc tttatttgtg aaatttagt
//
LOCUS       DBANUU010000273         3404 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000273 DBANUU010000000
VERSION     DBANUU010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.278"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..562
                     /gene="lpfB-O113"
                     /locus_tag="ACIE68_004737"
     CDS             <1..562
                     /gene="lpfB-O113"
                     /locus_tag="ACIE68_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFX5910898.1"
                     /translation="SVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQE
                     KILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIR
                     LIPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPA
                     NSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            587..3109
                     /gene="lpfC-O113"
                     /locus_tag="ACIE68_004738"
     CDS             587..3109
                     /gene="lpfC-O113"
                     /locus_tag="ACIE68_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFX5910899.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            3120..>3404
                     /gene="lpfD-O113"
                     /locus_tag="ACIE68_004739"
     CDS             3120..>3404
                     /gene="lpfD-O113"
                     /locus_tag="ACIE68_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFX5910900.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQAL"
BASE COUNT          938 a          745 c          824 g          897 t
ORIGIN      
        1 ttctgtattt agcaaagaag aaaaaatacc ttatctcatc caggcttggg tagatccatt
       61 taataaagag gataaaagca aagcgccgtt taccgttatt ccgccagtat cacgattaga
      121 accttcgcaa gaaaaaatat tacgcattat tcacaccaaa ggtgtgtcat tgcctgatga
      181 tcgcgagtca gtcttctggc tgaatattaa aaatattccg ccatcagcga gcaacaaagc
      241 gacgaactcg ctggaaatag cagtaaaaac gcgtatcaag ctgttctggc gaccagcaaa
      301 tatccgcctt attcccgaag atgctgcgcc gaaagtgaaa tggcgccggg aagggcgaaa
      361 tctgatagca gagaatccca acccaattca tatcagtgtg atggatgtca tcgttgatgg
      421 ccatgatgtg cccctcaata tgatccgtcc ttttgaaacg ctcacgttac ctttacctgc
      481 taattctgcg ggaggccaaa tgacctggcg atttattaat gattacggcg cagtcagtga
      541 cccgataaaa atgacgctgt aagcaaggga taaggatagg cgtattatga tgacgaccag
      601 aatagtggtt ggcctcacgg cagggacgtg tctgattttc tcgcaaaacc tgatggccga
      661 ggtcagtgta ttcaatccgg cgcttctgga aatcgaccat caatccggag tcgatattcg
      721 ccagtttaat cgggcaaacc tgatgccccc aggtgtttat agcgttgata tttttatcaa
      781 cggtaaaatg tttgaacgtc aggatgtgac atttgttcag gataatccag atgctgatct
      841 gcacgcttgc tttattgcca ttaaaaaaac actgtcctcc tttggcataa aagttgatgc
      901 gctcaaatcg ttcaatgatg tggatgagac ggtttgcctc gatcctgctc cacgtattga
      961 aggctcatcc tggcagtttg acagtgataa attgcagctg aatatatcca ttccccaaat
     1021 ctacatggac gcgatggctt atgattacat cagccccacg cgttgggatg aggggattaa
     1081 cgcgctcacc atcaactacg atttttctgg ttcacataca ctacgttcag attatggttc
     1141 acaagagaca gataccagtt atctcaatct gcgcaatgga ctgaatattg gaccgtggcg
     1201 gctacgtaat tacagtactt taaacaccag cgatggccgt gcggaataca actccattag
     1261 tacctggata cagcgcgata ttgccgcgtt aagaagccag attatgattg gtgatacgtg
     1321 gacggcgagc gatattttcg acagtacgca aattcgcggc gcgcgtttgt atactgataa
     1381 cgatatgcta cccgccagcc agaatggctt tgctcctgtg gttcgtggga ttgcaaagtc
     1441 caacgccacc gtcatcattc ggcagaatgg ctacgtgatt tatcagtctg ccgttccaca
     1501 aggtgctttt gagatcaccg atctcaacac cgccagtaca ggtggcgatt tggacgtaac
     1561 catcaaagaa gaagacggta gcgaacaacg attcacccaa ccttatgctt cattggcgat
     1621 tcttaaacgt gaaggtcaga cagatgttga tgtcagcgtg ggtgaattgc gcgatgaaga
     1681 cggatttaca ccggacgtcc ttcaggcgca aatacttcat ggtttttccc acgggatcac
     1741 tttatatgga ggtatgcagg ctgctgaaaa ttatggttct gcagctctgg gtgtcggtaa
     1801 agatcttggc gctttgggcg caatttcttt cgatgtgaca catgctcgtg cgaattttag
     1861 ccatgatgat acagaaacgg gtcagtcata tcgctttctc tattcaaaac gatttgacga
     1921 cacagacact agcttgcgcc tggttggcta tcgttactcc accgagggct actataccct
     1981 caatgagtgg gcatcgcggc gcaacagccc tgaagacttt tgggaaacag gtaaccgacg
     2041 tagtcgcgtg gagggaacgc taacgcagtc gttggggaga gattatggca atttatacct
     2101 gacattaagc cggcaacaat actggcatac cgatgatgtc gaacgattaa tgcaatttgg
     2161 ctacagcagt agctggaagc gtctctcgtg gaacgtctcc tggagttatt ccaatactgc
     2221 cagacagggg acggggaaca accatgccag tgataacacc agtgagcaga tctacatgct
     2281 ctctttatct gttcctttat cgggctggtg gggtaatagt tacgccacct attctgtttc
     2341 gcaaaacgat aattccggta gctcacatca actcggactc agcggtacgg cgctggaaag
     2401 aaataacctt tcatggaatt taatgcagtc ctataacagt catgatgatg aggttggcgg
     2461 taatatgtcc ctgacctatg atggctctta tggcacggtg aacggcagct ataactacag
     2521 ccaaaattcc cagaggctga attatggtat cagaggggga attctggcac acagcgaagg
     2581 ggtaacgtta agtcaggagt taggtgaaac tattgctctt gttaaagcac ctggggccgc
     2641 cgggttagaa atagataata tgcgcggtgc tgcgacggac tggcgcggct ataccgtcaa
     2701 gacacagcta aacccttatg atgaaaatcg ggtagcaatc agcgataact atttctcgaa
     2761 gtcgaatata gaacttgata ataccgtcgt tacgatggtt cccacgcgtg gtgcagtggt
     2821 taaagcggag tttgtgactc atgtgggtta tcgcgttctc ttcagagtgt taaatgcaaa
     2881 tggtaaaccg gtaccttttg gagccattgc tgcgatacaa gatgcaagtt tggcagattc
     2941 aggaattgtc ggtgaccgtg gcgaacttta tctttctggt ctaccagaaa aaggacaggt
     3001 tacgttatcc tggggagaaa acgcctcaac aaaatgcatc ttcaattatt cactttcgac
     3061 accagaaagt gagagcggat taattgaaca gggtgtgaca tgtcattaaa aggattttta
     3121 tgaacaagta tatcaaacag tggtgctttg ctgtgtttat gctctcgtta agtagtgtag
     3181 cacttgcagc tcctaaaggt atctgtaccc ctgataatgg cgtatttcac agtacgcttg
     3241 atttctcggg atatcttatt accgcgaacg aaaataaggt tggaacgaca tttaacacca
     3301 ctgtaactaa tggatcctca tatcctggac gctgtcattg tgatactggt aacgtaggag
     3361 aattccccta tatctattat acgtcaaaaa taaaccaggc atta
//
LOCUS       DBANUU010000274         3388 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000274 DBANUU010000000
VERSION     DBANUU010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.280"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            122..1087
                     /gene="dusB"
                     /locus_tag="ACIE68_004740"
     CDS             122..1087
                     /gene="dusB"
                     /locus_tag="ACIE68_004740"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFX5910901.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1113..1409
                     /gene="fis"
                     /locus_tag="ACIE68_004741"
     CDS             1113..1409
                     /gene="fis"
                     /locus_tag="ACIE68_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFX5910902.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1495..2379
                     /gene="yhdJ"
                     /locus_tag="ACIE68_004742"
     CDS             1495..2379
                     /gene="yhdJ"
                     /locus_tag="ACIE68_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFX5910903.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NRSKRCRVSEVDPDLIAK"
     gene            2463..2642
                     /locus_tag="ACIE68_004743"
     CDS             2463..2642
                     /locus_tag="ACIE68_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFX5910904.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(2645..3307)
                     /gene="acrS"
                     /locus_tag="ACIE68_004744"
     CDS             complement(2645..3307)
                     /gene="acrS"
                     /locus_tag="ACIE68_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFX5910905.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT          928 a          781 c          803 g          876 t
ORIGIN      
        1 cattttaaat gcaattcttt gatccatctc agaggattgg tcaaagtttg gcctttcatc
       61 tcgtgcaaaa aatgcgtaat atacgccgcc ttgcagtcac agtatggtca tttcttaact
      121 catgcgcatc ggacaatatc agctcagaaa tcgcctgatc gcagcgccca tggctggcat
      181 tacagacaga ccttttcgga cgttgtgcta cgagatggga gccggattga cagtatccga
      241 gatgatgtct tctaacccac aggtttggga aagcgacaaa tctcgtttac ggatggtgca
      301 cattgatgaa cccggtattc gcaccgtgca aattgctggt agcgatccga aagaaatggc
      361 agatgcagca cgtattaacg tggaaagcgg tgcccagatt attgatatca atatgggttg
      421 cccggctaaa aaagtgaatc gcaagctcgc aggttcagcc cttttgcagt acccggatgt
      481 cgttaaatcg atccttaccg aggtcgtcaa tgcagtggac gttcctgtta ccctgaagat
      541 tcgcaccggc tgggcaccgg aacaccgtaa ctgcgaagag attgcccaac tggctgaaga
      601 ctgtggcatt caggctctga ccattcatgg ccgtacacgc gcctgtttgt tcaatggaga
      661 agctgagtac gacagtattc gggcagttaa gcagaaagtt tccattccgg ttatcgcgaa
      721 tggcgacatt actgacccgc ttaaagccag agctgtgctc gactatacag gggcggatgc
      781 cctgatgata ggccgcgcag ctcagggaag accctggatc tttcgggaaa tccagcatta
      841 tctggacact ggggagttgc tgcccccgct gcctttggca gaggttaagc gcttgctttg
      901 cgcgcacgtt cgggaactgc atgactttta tggtccggca aaagggtacc gaattgcacg
      961 taaacacgtt tcctggtatc tccaggaaca cgctccaaat gaccagtttc ggcgcacatt
     1021 caacgccatt gaggatgcca gcgaacagct ggaggcgttg gaggcatact tcgaaaattt
     1081 tgcgtaaaca gaaataaaga gctgacagaa ctatgttcga acaacgcgta aattctgacg
     1141 tactgaccgt ttctaccgtt aactctcagg atcaggtaac ccaaaaaccc ctgcgtgact
     1201 cggttaaaca ggcactgaag aactattttg ctcaactgaa tggtcaggat gtgaatgacc
     1261 tctatgagct ggtactggct gaagtagaac agcccctgtt ggacatggtg atgcaataca
     1321 cccgtggtaa ccagacccgt gctgcgctga tgatgggcat caaccgtggt acgctgcgta
     1381 aaaaattgaa aaaatacggc atgaactaat tcaggttagc taaatgcttg attaaaaagg
     1441 cgctactcgg catggggaag cgcctttttt ataggtgtca caaagggagt gaccatgaga
     1501 acaggatgtg aaccgacccg gtttggtaat gaagctaaga ccattattca cggtgatgcc
     1561 cttgccgaac ttaaaaagct acctactgaa agcgtcgatc tgatctttgc cgacccaccg
     1621 tataacatcg gtaaaaattt tgatggtctg atcgaagcct ggaaagaaga tctgtttatc
     1681 gactggctgt ttgaagtgat tgcagagtgc caccgcgttc tgaaaaagca gggcagcatg
     1741 tacattatga acagtacgga aaacatgccc tttatcgatc tccagtgccg caagcttttt
     1801 accatcaaaa gtcgcatcgt ctggtcatat gacagttctg gagtacaggc gaaaaaacac
     1861 tacggctcca tgtacgaacc catcctgatg atggtgaaag acgcaaagaa ctacacattc
     1921 aacggtgatg ctattctggt agaagccaaa accggatcgc agcgcgcgtt gatcgattat
     1981 cgcaaaaatc ctccacagcc atacaatcat caaaaagtac cgggtaacgt ctgggatttt
     2041 ccgcgcgtgc gttatttaat ggatgaatat gagaaccacc cgacgcaaaa accggaagcc
     2101 ttactgaaac gcattattct cgcctcttcc aacccaggcg atatcgttct cgacccgttt
     2161 gccggtagct ttactaccgg tgccgtagcc atcgccagcg gacgaaaatt cattggtatt
     2221 gagatcaaca gcgagtacat caaaatgggg cttcgacggc tggatgtcgc gtcgcattac
     2281 tccgcggaag aactggcgaa agtgaaaaaa agaaagacgg gcaaccggtc aaaacgatgc
     2341 cgggttagcg aagttgaccc cgatctcatt gcaaagtaaa ggaagtgtaa gcctggtttt
     2401 cagattattc atttcgtgta tatttcctgc cagacttggt taaacatgca caggctcagg
     2461 taatgattcg caagtattgg tggctcgtcg ttttcgctgt cttcgttttt ctgtttgata
     2521 ctttactgat gcagtggatt gaactgctgg caacagaaac agacaaatgc cgcaatatga
     2581 actcagttaa tccactaaaa ctggttaact gtgacgaact gaattttcag gacagaatgt
     2641 gaatttacat gacacttaat tcattcgttt gatgaattaa tttcgttatg ttttcatctg
     2701 gcatgaacat tcttaatacg ttatcgacca gcgcgggtgc ttgtttataa agatcataac
     2761 ccgccatatt cattaaccag ttttgaacaa ttccgctgaa ggcaccatca ataataatca
     2821 tcacaacatc taaatcgagg ttatttgcta cacaaccttg ttgctgacac gcctgcaata
     2881 cttcgcggag agtctgcgga ttaaagccca tcttttcgcg tatcactccc tcggccagca
     2941 tctcatcatt aaattcacat ttgtgatata agattttcag caacgcctgc tggcggggaa
     3001 ttttggcaat atattgcaag ccgacaatca atttttcacg caattgttga aacgggtcat
     3061 gctctaatcc agccgtcaag tgttcctgga ttaactcccg caatgaaggc tgttgcaacc
     3121 acatctcatt aaacagttga gtcttgtttt cgaagtgcca gtagatagcg ccacgcgtaa
     3181 cgttagcagc gtcggcaatg tcgttgagtg tcgtcttgct tacgccatgc tgcgcaaact
     3241 gggcgatggc agtttcaatc agttcttgcc gggtcttcag agcttcggct ttggttcttt
     3301 ttgccatgat taattattca ggaaataaat atattcgaca cagagtgaga aaatagcgaa
     3361 ggttaatcta tcacctaatg tgtattta
//
LOCUS       DBANUU010000275         3364 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000275 DBANUU010000000
VERSION     DBANUU010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.281"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            341..1657
                     /gene="shiA"
                     /locus_tag="ACIE68_004745"
     CDS             341..1657
                     /gene="shiA"
                     /locus_tag="ACIE68_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFX5910906.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGTLIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQRA"
     gene            1759..3213
                     /gene="amn"
                     /locus_tag="ACIE68_004746"
     CDS             1759..3213
                     /gene="amn"
                     /locus_tag="ACIE68_004746"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFX5910907.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          775 a          826 c          810 g          953 t
ORIGIN      
        1 cgtctcaaca atgatgctaa aaacatacct aacctcgcct ccctactggt tataatgcaa
       61 tgcagtctat cagactcatc agggtgccat tttgtgcata tgcggacttt tatgtttcat
      121 atctctaacc tgtgggtcct ctgcttaatc cttaaacaac accagcaact cctgcgcttt
      181 catcttccat cgaatttttc atgttgccgc taatcagcca taaaatcatt tgcagatgcg
      241 ctctgtcgag gtagtctcat aaggttcgtt tatagatcga cggcaatgtg agttaccttt
      301 tccatactaa ttataaaaag acagtacaaa caggatcatt atggactcca cgctcatctc
      361 cactcgtccc gatgaaggga cgctttcgtt aagtcgcgcc cgacgagctg cgttaggcag
      421 cttcgctggt gccgtcgtcg actggtatga ttttttactc tatggcatca ccgccgcact
      481 ggtgtttaat cgcgagtttt tcccgcaagt aagcccggcg atgggaacgc tcgccgcatt
      541 tgctaccttt ggcgtcggat ttcttttccg tccgctcggc ggtgtcattt tcggtcactt
      601 tggcgaccga ctgggacgta agcgcatgtt aatgctgacc gtctggatga tgggcatcgc
      661 gacagccttg attggtattc ttccttcatt ctcgaccatt gggtggtggg cacctatttt
      721 gctggtgaca ctgcgtgcca ttcagggatt tgcagtcggc ggcgaatggg gaggcgcggc
      781 gttgctttcc gttgaaagtg caccgaaaaa taaaaaagcc ttttacagta gcggtgtaca
      841 agttggctac ggtgtaggtt tactgctttc aaccggactg gtttcattga tcagtatgat
      901 gacgactgac gaacagtttt taagctgggg ctggcgcatt cctttcctgt ttagcatcgt
      961 actggtactg ggagcattgt gggtgcgcaa tggcatggag gagtccgcgg aatttgaaca
     1021 acagcaacat tatcaagctg ccgcgaaaaa acgcatcccg gttatcgaag cgctgttacg
     1081 acatcccggt gctttcctga agattattgc gctacgactg tgcgaattgc tgacgatgta
     1141 catcgttact gcctttgcac ttaattattc aacccagaat atggggctac cgcgcgaact
     1201 tttccttaat attggtttgc tggtaggtgg attaagctgc ctgacaattc cctgttttgc
     1261 ctggcttgcc gatcgttttg gtcgccgtag ggtttatatc acaggtacgt taatcggaac
     1321 gttgagcgca tttcctttct ttatggcgct tgaagcacaa tctattttct ggatagtttt
     1381 cttctccata atgctggcaa acattgcgca tgacatggtg gtgtgtgtgc aacaaccgat
     1441 gtttaccgaa atgtttggtg ccagttatcg ctatagtggc gctggagtcg gttatcaggt
     1501 tgccagtgtg gttggcggtg gatttacacc ttttattgcc gctgcactca tcacttactt
     1561 tgccgggaac tggcatagcg tcgccattta tttgctggct ggatgcctga tttccgcaat
     1621 gaccgctttg ttgatgaaag acagtcaacg cgcttgatag cctggcgaag atcatccgat
     1681 cttcgcctta cacttttgtt tcacatttct gtgacatact atcggatgtg cggtaattgt
     1741 atggaacagg agacacacat gaataataag ggctccggtc tgaccccagc tcaggcactg
     1801 gataaactcg acgcgctgta tgagcaatct gtagtcgcat tacgcaacgc cattggcaac
     1861 tatattacaa gtggcgaatt acctgatgaa aacgcccgca aacaaggtct ttttgtctat
     1921 ccatcactga ccgtaacctg ggacggtagc acaaccaatc cccccaaaac gcgcgcattt
     1981 ggtcgcttta cccacgcagg cagctacacc accacgatta ctcgccctac tctctttcgt
     2041 tcgtatctta atgaacaact tacgttgctg tatcaggatt atggtgcgca tatctcagtg
     2101 caaccctcgc agcatgaaat cccttatcct tatgtcatcg atggctctga attgacactt
     2161 gatcgctcaa tgagcgctgg gttaactcgc tacttcccga caacagaact ggcgcaaatt
     2221 ggcgatgaaa ctgcagacgg catttatcat ccaactgaat tctccccgct atcgcatttt
     2281 gatgcgcgcc gcgtcgattt ttccctcgca cggttgcgcc attataccgg tacgccagtt
     2341 gaacattttc agccgttcgt cttgtttacc aactacacac gttatgtgga tgaattcgtt
     2401 cgttggggat gcagccagat cctcgatcct gatagtccct acattgccct ttcttgtgct
     2461 ggcgggaact ggatcaccgc cgaaaccgaa gcgccagaag aagccatttc cgaccttgca
     2521 tggaaaaaac atcagatgcc agcatggcat ttaattaccg ccgatggtca gggtattact
     2581 ctggtgaata ttggcgtggg accgtcaaat gctaaaacca tctgcgatca tctggcagtg
     2641 ctacgcccgg atgtctggtt gatgattggt cactgtggcg gattacgtga aagtcaggcc
     2701 attggcgatt atgtacttgc acacgcttat ttacgcgatg accacgttct tgatgcggtt
     2761 ctgccgcccg atattcctat tccgagcatt gctgaagtgc aacgtgcgct ttatgacgcc
     2821 accaagctgg tgagcggcag gcccggtgag gaagtcaaac agcggctacg tactggtact
     2881 gtggtaacca cagatgacag gaactgggaa ttacgttact cagcttctgc acttcgtttt
     2941 aacttaagcc gggccgtagc aattgatatg gaaagtgcaa ccattgccgc gcaaggatat
     3001 cgtttccgcg tgccatacgg gacactactg tgtgtttcag ataaaccgtt gcatggcgag
     3061 attaaacttc ccggccaggc taaccgtttt tatgaaggcg ctatttccga acatctgcaa
     3121 attggcattc gggcgatcga tttgctgcgc gcagaaggcg accgactgca ttcgcgtaaa
     3181 ttacgaacct ttaatgagcc gccgttccga taataagttc tgaaccttct tatcagaaat
     3241 agatgtgaag gagtaagtaa gacacctggc aaatagcctg caataatcgt gggctgtttg
     3301 cttccttggg cggatacgag ttttattatc gtcttaatga tttccacata ttaaaagcaa
     3361 gtat
//
LOCUS       DBANUU010000276         3349 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000276 DBANUU010000000
VERSION     DBANUU010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.282"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            88..1086
                     /gene="yiaK"
                     /locus_tag="ACIE68_004747"
     CDS             88..1086
                     /gene="yiaK"
                     /locus_tag="ACIE68_004747"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFX5910908.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            1098..1565
                     /locus_tag="ACIE68_004748"
     CDS             1098..1565
                     /locus_tag="ACIE68_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFX5910909.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            1683..2156
                     /gene="yiaM"
                     /locus_tag="ACIE68_004749"
     CDS             1683..2156
                     /gene="yiaM"
                     /locus_tag="ACIE68_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFX5910910.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            2159..>3349
                     /gene="yiaN"
                     /locus_tag="ACIE68_004750"
     CDS             2159..>3349
                     /gene="yiaN"
                     /locus_tag="ACIE68_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFX5910911.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVF"
BASE COUNT          775 a          815 c          838 g          921 t
ORIGIN      
        1 taaaaagtga tcgatatatt tgaaatcaag tttcgcatat tgaaatttta agccaaaaaa
       61 gcgttcaaaa aaacaaggaa gcctgggatg aaagtgacat ttgagcagtt aaaagcagcc
      121 tttaatcggg tcttaatttc acgcggcgtt gacagcgaaa cggctgacgc ctgtgcagag
      181 atgttcgccc gcaccaccga atccggcgtt tattctcacg gcgttaatcg tttccctcgt
      241 ttcattcaac aactggaaaa cggcgatatc attcctgatg cccaacccaa acgtataacc
      301 agcctcggcg caattgaaca gtgggatgcc cagcgttcga tcggcaacct gacggcgaaa
      361 aagatgatgg atcgcgccat tgaactggct gccgatcacg gtattggtct ggtggcactt
      421 cgtaatgcca accactggat gcgcggcggc agctacggct ggcaggcggc ggaaaaaggc
      481 tatattggca tttgctggac caactccatc gccgtaatgc cgccgtgggg cgcaaaagag
      541 tgtcgcattg gcactaaccc gctgatcgtc gccattcctt ccacgccgat caccatggtc
      601 gatatgtcga tgtcgatgtt ctcttacggc atgttagaag ttaaccgcct ggcgggccgt
      661 cagctcccgg tcgatggtgg ctttgatgat gagggcaatt tgaccaaaga acctggcgtt
      721 atcgagaaga atcgccgcat tttgccgatg ggctactgga aaggttctgg catgtcgatt
      781 gtgctggata tgatcgctac tctcctttcc gacggcgcat cggttgccga agtcacccag
      841 gacaacagcg acgaatacgg catttcacaa atttttattg ccattgaagt ggacaagctt
      901 atcgacggtc ccacccgcga tgccaagctg caacgtatca tggattacgt tactaccgct
      961 gaacgcgctg acgaaaacca ggccatccgc ttacccggcc acgaatttac taccctgctg
     1021 gccgaaaacc gccgtaacgg tattaccgtt gatgacagcg tgtgggccaa aatccaggcg
     1081 ttataaggag ataagtcatg atttttggac atatcgcgca gcccaatccg tgccgtttgc
     1141 ccgccgccat tgaaaaggcg ctcgattttc tgcgcgctac cgatttcaac gccctggagc
     1201 cgggcgttgt cgaaatcgac ggcaaaaata tttatgcgca aattatcgat ttaaccacac
     1261 gcgaagcggt ggaaaaccgt ccggaagtgc atcgtcggta tatcgatatt cagtttctgg
     1321 cgtggggtga agagaaaatc ggcattgcta ttgatacggg aaataataaa gtcagcgaat
     1381 cactattaga gcagcgcgat attatttttt atcatgacag tgaacatgaa tcatttatag
     1441 aaatgatacc gggcagctac gcaattttct ttccgcagga tgttcaccga ccaggttgta
     1501 ttttgcaaac agcctctgag atacgaaaaa ttgtggttaa agtcgcatta acggcgctga
     1561 attaatcgaa taatcgtcta cgccagaacg actgggtaat gttattgctc taactatatt
     1621 aataatttag gttgctgcac atgcaacttg aattatgaca gacatagcct caaggaatag
     1681 ctatgaaaaa aatacttgaa gcaatactgg cgattaatct cgccgtactt tcctgtattg
     1741 tgtttataaa cattatttta agatatggtt ttcagacaag cattttatct gtagatgaat
     1801 tgtcacgtta tttatttgtc tggttaacat ttattggcgc gattgtggct tttatggata
     1861 acgcccacgt tcaggtcacc tttctggtgg agaaactctc ccccgcatgg caacgacgag
     1921 tcgctctggt tacccattcc ttaatcttat ttatttgtgg cgcactggcc tggggcgcaa
     1981 cgctaaaaac aattcaggac tggagcgatt attcaccgat cctcggttta cccatcggcc
     2041 tgatgtatgc cgcttgtctg cccaccagcc tcgttatcgc attctttgaa ctgcgtcatt
     2101 tatatcaact catcacgcgt agcaattctc taacgtcgcc accgcaagga gcttaatcat
     2161 ggctgtgctg atttttctgg gctgtctgtt gggtggtatt gctatcggtt tgcctattgc
     2221 ctgggcactg ttgttgtgcg gggcggcgtt aatgttctgg ctggacatgt ttgatgtcca
     2281 gatcatggca caaacgctgg tgaacggagc cgatagcttc tccctgctgg cgattccgtt
     2341 ctttgtgctg gcgggtgaaa tcatgaatgc gggcggcttg tcaaaacgca ttgttgacct
     2401 gccgatgaaa ctggtggggc ataaaccagg cgggctgggc tacgtcggcg tgctggcagc
     2461 tatgattatg gcgagccttt ctggttctgc cgttgcggat accgccgccg ttgccgctct
     2521 actggtgccg atgatgcgca gcgccaacta cccggtcaac cgggcggcgg ggctgattgc
     2581 ctctggcggc attatcgcgc caattattcc cccttccatt ccgtttatta tcttcggcgt
     2641 ttccagcgga ttatccatca gtaagctgtt tatggcgggc attgcccctg gcatgatgat
     2701 gggcgcaacg ctgatgctta cctggtggtg gcaggccagt cgccttaatt taccccgcca
     2761 gcaaaaagca acgatgcagg aaatctggca ctcttttgtc tccggtatct gggcgctgtt
     2821 tcttccagtc atcattattg gcgggtttcg ctccggtctg tttaccccta ccgaagccgg
     2881 ggccgttgct gctttctatg cgctgtttgt cgccacagtt atttaccgtg aaatgacctt
     2941 cgccacactt tggcatgtac tcattggcgc ggcgaaaacc acctcagtgg ttatgtttct
     3001 ggtggcctcg gcacaagttt ccgcctggct gattaccatt gctgaactgc cgatgatggt
     3061 ttctgattta ctgcaaccgt tggtcgattc accgcgtctg ctgtttatcg tcattatggt
     3121 ggctatcctg attgtcggca tggtcatgga tttaacgccg acggtattaa ttcttacccc
     3181 ggtattaatg cctttagtta aagaggcagg aatcgatccg atttatttcg gtgtcatgtt
     3241 tatcattaac tgctcaatcg gcttaattac accgcctatc ggtaatgtac ttaacgttat
     3301 ttcaggggtg gcaaaactca aattcgatga tgcagtcaga ggcgttttc
//
LOCUS       DBANUU010000277         3335 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000277 DBANUU010000000
VERSION     DBANUU010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.283"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            20..241
                     /gene="traR"
                     /locus_tag="ACIE68_004751"
     CDS             20..241
                     /gene="traR"
                     /locus_tag="ACIE68_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFX5910912.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            401..3028
                     /gene="traC"
                     /locus_tag="ACIE68_004752"
     CDS             401..3028
                     /gene="traC"
                     /locus_tag="ACIE68_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFX5910913.1"
                     /translation="MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLL
                     PYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLMS
                     SQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYRV
                     FISYCSPSKKKSRADILEMENLVKIIRASLQGASITTQTVDAQAFIDIVGEMINHNPD
                     SLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRESGRNSTARILNFHLARNPE
                     IAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSY
                     AKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFR
                     KNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNP
                     LTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGG
                     FAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNG
                     NLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIV
                     LLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRM
                     YRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSD
                     KASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFSS
                     FLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSGP
                     EMASLEAWLEEHEKYRSVA"
     gene            3025..>3335
                     /gene="trbI"
                     /locus_tag="ACIE68_004753"
     CDS             3025..>3335
                     /gene="trbI"
                     /locus_tag="ACIE68_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFX5910914.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAISYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PA"
BASE COUNT          825 a          852 c          919 g          739 t
ORIGIN      
        1 atgtcgggag gaatattccg tgagtgatga agccgatgaa gcatattcag tgacagaaca
       61 actgaccatg acaggaataa accggatacg ccagaaaata aatgctcatg gtattcctgt
      121 ttatctctgt gaagcatgcg gaaatcctat tccggaagcc cggcggaaaa tatttcccgg
      181 tgtgacgttg tgcgttgaat gtcaggcgta tcaggaaaga cagagaaaac attatgcata
      241 agtcagtcgc agaacatagt gatttaattc cggatgaaca tgagtggata ttcagaaaac
      301 agaaatcttt gtatatgcgc cgggaaatgg cgcgttaatt acaggtattc ccttcatggc
      361 taccgcattt ctcgctttat ttttcaacta aggaattcat gtgaataacc cacttgaggc
      421 cgtcactcag gcggttaact ccctcgtcac agcactgaaa ctgcctgacg aatccgcaaa
      481 ggccaatgaa gttctgggcg aaatgagctt cccgcagttc agccgtctgc tgccgtaccg
      541 tgattacaac caggaatccg gtctgttcat gaatgacacc acgatgggct ttatgctgga
      601 agccattccc atcaatgggg cgaatgagtc cattgtggag gccctcgatc acatgctgcg
      661 caccaaactg ccgcgcggta ttccgttgtg tatccatctg atgtccagtc agctggttgg
      721 tgacaggatt gaatacgggc tgcgtgagtt ctcctggtca ggtgaacagg ctgaacggtt
      781 taacgccatt acccgggcct attatatgaa agcggcagcg acacagtttc cgctgccgga
      841 ggggatgaat ctgcccctga ccctgcgcca ttaccgggtg tttatctcgt actgttctcc
      901 ttcgaagaaa aaaagccggg ccgacattct ggaaatggaa aacctggtga aaatcatccg
      961 ggcgtcgtta cagggggcca gtatcaccac acagacggtg gatgcacagg cctttatcga
     1021 tattgtcggg gagatgatta accataaccc ggactccctg tacccgaaaa gacgccagct
     1081 ggacccgtat tctgatctga attatcagtg tgtggaggac agttttgacc tgaaagtccg
     1141 ggctgattac ctgacgctgg gcctgcgtga gagcggcagg aacagtacgg cccgcatcct
     1201 gaatttccat ctggcccgta acccggaaat cgcttttttg tggaacatgg ccgacaacta
     1261 cagcaacctg ctgaacccgg agttgtccat ctcctgcccg ttcatcctga cgctgactct
     1321 ggtggtggaa gaccaggtga aaacccacag cgaagccaac ctgaagtaca tggacctgga
     1381 gaaaaagtcg aagacctcct atgccaaatg gtttccgtcc gtggagaaag aagcgaagga
     1441 gtggggggaa ctgcgtcagc ggctgggctc cggtcagtcc tctgtcgtgt cctacttcct
     1501 caacatcaca gccttctgca aggacaataa tgaaacggca ctggaagtgg agcaggacat
     1561 cctgaacagc ttccgtaaaa acggttttga gctgatttca ccgcgcttta accacatgcg
     1621 caatttcctg acctgtctgc cctttatggc cgggaaaggg ttgtttaaac agctgaaaga
     1681 ggccggagtg gtacagcgcg cagagagctt taatgtggcc aacctgatgc cgttagtggc
     1741 ggataacccc ctgacaccgg caggtctgct ggcacccacc taccgtaacc agctggcgtt
     1801 tatcgatatt ttcttccggg ggatgaataa caccaactat aacatggcgg tctgtggcac
     1861 ctccggggcc ggtaaaaccg ggctgataca gccacttatc cgcagcgtgc tggactccgg
     1921 aggctttgcc gtggtgttcg acatggggga tggatacaag tccctgtgtg agaacatggg
     1981 cggggtgtat ctggacggtg aaaccctgcg ctttaacccg tttgcgaaca tcaccgatat
     2041 tgaccagtca gcggagcgtg tccgtgacca gctgtcggtg atggccagcc ccaacggtaa
     2101 cctggatgaa gtgcatgaag gtctgctgct gcaggcagtc agggcatcat ggctggccaa
     2161 agagaacagg gcacgtattg atgacgtggt ggatttcctg aaaaacgcca gtgacagcga
     2221 gcagtatgcc gagtcaccga ccatccgcag ccgtctggac gaaatgattg tgctgcttga
     2281 ccagtacact gctaacggca cttatggcca gtatttcaac tccgatgagc cgtccctgcg
     2341 ggatgatgcg aaaatggtgg tgctggagct gggcggactg gaagaccgtc cgtccctgct
     2401 ggttgcggtg atgttcagcc ttatcattta cattgagaac cggatgtacc gcacgccgcg
     2461 taacctcaag aaactgaacg tcattgatga aggctggcgt ctgctggact tcaaaaatca
     2521 caaggtcggt gaatttattg agaaaggcta ccgtaccgcc cgccgtcata ccggtgccta
     2581 tatcaccatc acccagaaca tcgtcgactt tgactctgac aaggcctcca gtgcggcccg
     2641 tgcagcatgg ggtaactcct cctacaaaat tatcctcaaa cagagtgcga aggagttcgc
     2701 gaaatacaac cagctgtatc cggaccagtt cctgccactg cagcgtgaca tgattggtaa
     2761 gttcggtgcg gccaaagatc agtggttcag ttccttcctg ctgcaggtgg aaaaccattc
     2821 ctcctggcac cgcctgtttg tggacccgtt aagccgcgcc atgtacagct ctgacggccc
     2881 ggattttgag tttgtgcagc agaaacgtaa agaggggctg agtattcatg aggcagtgtg
     2941 gcagctggcg tggaagaagt cagggccgga gatggcttcg ctggaagcct ggctggaaga
     3001 acatgagaaa tacaggagtg ttgcatgacc acaacgcaaa aaacgactga cgttacagcc
     3061 ccccgacgca gccactggtg gtggacggtg ccgggatgcc tggcaatggt gttactgaac
     3121 gcagccatca gttacggcat tgtcagactg aacgcaccgg tgactgccgc cttcaacatg
     3181 aaacagaccg tggatgcgtt ttttgacagc gccagtcaga aacaactgtc agaagcacag
     3241 tcaaaagccc tttcagcgcg ttttaacacg gcactggaag ccagcctgca ggcatggcag
     3301 cagaaacacc acgcggtcat tctggtgtcg cctgc
//
LOCUS       DBANUU010000278         3327 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000278 DBANUU010000000
VERSION     DBANUU010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.284"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            117..635
                     /locus_tag="ACIE68_004754"
     CDS             117..635
                     /locus_tag="ACIE68_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFX5910915.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            845..2986
                     /locus_tag="ACIE68_004755"
     CDS             845..2986
                     /locus_tag="ACIE68_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFX5910916.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGMVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQESSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3062..>3327
                     /locus_tag="ACIE68_004756"
     CDS             3062..>3327
                     /locus_tag="ACIE68_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFX5910917.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPV"
BASE COUNT          811 a          856 c          976 g          684 t
ORIGIN      
        1 atgtaaaaaa tactgaaaaa tgatgcggtt ccgtgaagtg agctgcatct gcctgtctgg
       61 gattaacggc aggtcattgt aagtagtaat taaccacgtt aacaaggagt ttacaaatgc
      121 caacaccgtg ttatatctcc atcactggtc agacccaggg aaatattact gcaggtgcat
      181 ttaccgctga ttctgtcggc aacatctatg tgcaggggca cgaagacgag atgctggtgc
      241 aggaattcct gcataacgtt actgtcccga ccgatcctca gtccggtcag cctgcgggtc
      301 agcgtgctca caagcctttc atttttaccg tggcgctgaa caaagcggtt ccgctgatgt
      361 acaacgcact ggcctccggc gaaatgctgc caaccactga acttcactgg tggcgtacct
      421 ctgtggaagg caaacaggag cattatttca ctacccgcct gactgattcg acgattgtcg
      481 atatgaaact gcatatgccg cattgccagg acccggcgaa acgcgagttc acccagttgc
      541 tcgaagtttc actggcctac cgcaaaattg aatgggaaca cgtcaaatct ggcacctccg
      601 gtgccgacga ctggcgcgca ccgctggaag cataagttaa gccaacagca tccggctatc
      661 aggccggatg ctgtttttgt attacgccgt agaaagaaag gttgtcagta aggggttacg
      721 ttcacgggag atggcggctg ccggaaactt cggcggtagc gtgcctgaac gcaagcgtaa
      781 cactgtactg tcacagccgc tttaacagcg aactgtctgt ttttccttaa ccccggaggt
      841 tgctatgtca accggattac gtttcacgct ggaagtggac ggcctgccac cggatgcttt
      901 tgcggtggtc tcctttcatc tgaaccagtc actctcttcg cttttttccc tcgatctctc
      961 tctggtcagc cagcagtttc tctcccttga attccagcag atcctcgaca aaatggccta
     1021 cctgacgata tggcagggcg atgacgtaca gcgccgggtg aaaggtatgg tgacctggtt
     1081 tgaactaggg gagaacgaca aaaaccagat gctgtacagc atgaaggtgt gcccaccgct
     1141 gtggcgcaca gggctgcgcc agaacttccg tatcttccag aatgaggaca tcgaaagcat
     1201 cctcggcacg atattgcagg aaaacggggt gaccgagtgg agcccgctgt tcagcgagcc
     1261 acatccttcc cgtgagtttt gtgtccagta cggtgagact gattacgatt tcctgtgccg
     1321 gatggcggcg gaggaaggca tcttctttta tgaggagcac gcgcaaaaaa gtaccgacca
     1381 gagcctggtc ctgtgcgata ccgtgcgtta tctgccggag tcctttgaga tcccctggaa
     1441 tccgaacacc cgtaccgagg tgagcaccct ctgcatcagc cagtttcgct acagcgcaca
     1501 aatccgccct tcttccgtgg tgaccaaaga ctacaccttt aaacgacccg gctgggcagg
     1561 gcgttttgat caggaaggcc agtaccagga ttaccagcgc acacagtatg aagtgtatga
     1621 ctaccccgga cgtttcaagg gtgcccacgg gcagaacttt gcccgctggc agatggatgg
     1681 ctggcgcaac aacgcagaag tggcgcgcgg aacaagccgt tcgccggaga tatggccggg
     1741 acggcgaatt gtgctgacgg ggcatccgca ggcgaacctg aaccgggaat ggcaggtggt
     1801 ggcgagcgat cttcacggcg aacagccgca ggcggtaccg ggacgcaggg gttcaggcac
     1861 cacgctggat aaccactttg cggtaatccc ggcagaccgg acatggcggc cacagccgtt
     1921 gctgaaaccg ctggtggacg gcccgcagag cgccgtcgtg acgggaccgg caggcgagga
     1981 aatcttctgt gatgaacatg gccgcgtgcg ggtgaaattt aactgggacc gctataaccc
     2041 gtcaaaccag gaaagttcat gctggatccg tgtggcacag gcgtgggcag gcaccggctt
     2101 tggtaacctg gcgataccgc gtgtgggtca ggaggtcatt gtggacttcc tcaacggcga
     2161 tccggaccag ccgatcatta tggggcgtac ctaccaccag gaaaaccgca cccccggcag
     2221 cctgccgggg acgaagacgc agatgacgat ccgctcgaaa acctataagg gcagcgggtt
     2281 taatgaactg aagtttgacg atgcgacagg gaaagaacag gtctacatcc acgcgcagaa
     2341 gaacatgaac accgaggtgc tgaataaccg caccactgat gtgataaaca accatgccga
     2401 aaaaataggt aacaaccagg cgatcaccgt taccaataac cagatccaga acattggcgt
     2461 taatcagata cagacggttg gtgtcaacca ggtggaaacg gtgggcagta accagattat
     2521 caaagtggga tcaaaccagg ttgaaaaggt ggggatcatt cgtgcgctga cggtgggtgt
     2581 ggcttaccag acgacggtag gcggcattat gaatacctcg gtggcgttgt tgcagtcctc
     2641 acaggtaggg ctgcataaat cactgatggt gggaatgggc tacagcgtca atgtggggaa
     2701 taacgtcacc ttctcggtgg gcaagacaat gaaggaaaac accggacaaa cagcagttta
     2761 ttctgccggt gagcatcttg aactctgctg tggtaaggca aggctggtgc tgacgaagga
     2821 cggaagcata tttctcaatg gtacgcacat tcatctggaa ggggagtcgg atgtgaacgg
     2881 tgatgcgcca gtgattaact ggaactgtgg tgccacacaa cctgtaccgg atgcgcctgt
     2941 gccgaaagat ttaccccccg gaatgccgga tatgcggcaa ttttgagtca ggtgcaaaaa
     3001 attaaaacgg aactggtgca gtgaccagat aaaccatatt aaaagcagaa ggaattgatt
     3061 catgggcgga aaaccggcgg cgcggcaggg tgatatgacc cgcaagggac tggatattgt
     3121 gcagggttca gcgggggtgc tgataggtgc gccgacggga gtggcctgtt cggtatgtcc
     3181 gggagggatt acctatgcta acccggtgaa cccgctgctg ggtgcgaagg tgctgccggg
     3241 cgagacggac cttgcgctgc ccggcccgct gccgtttatt ctttcccgcg cctacagcag
     3301 ctaccggacc agaacacccg cgccggt
//
LOCUS       DBANUU010000279         3320 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000279 DBANUU010000000
VERSION     DBANUU010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.285"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..480
                     /locus_tag="ACIE68_004757"
     CDS             <1..480
                     /locus_tag="ACIE68_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308915.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFX5910918.1"
                     /translation="ELIRGTPIMVQVMFIYFALPMILPVRIDPITAAMVTIIINSGAY
                     IAEITRGAILSINRGFKEASLAMGLSQRATLWHVIMPLALRRMIPALGNQWIISIKDT
                     SLFIVIGVAELTRQGQEIIAGNFRALEVWTAVALIYLAVTLCLSFLLKQLEKRIHIL"
     gene            483..1205
                     /gene="glnQ"
                     /locus_tag="ACIE68_004758"
     CDS             483..1205
                     /gene="glnQ"
                     /locus_tag="ACIE68_004758"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFX5910919.1"
                     /translation="MVEFSAVAKHFGTTQVLYDINLKIDAGEVVVIIGPSGSGKSTLL
                     RCINKLEEISSGTLLVAGMHLTDPHVNECEIRREAGMVFQQFHLFPHLTALENVMFGP
                     IRVRKMSQAAAREQALALLERVGLGERAEHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMRSLADEGMTMVIVTHEIGFARDVASRLIFIDGGTIAEDG
                     PPDILLNASSNPRLKEFLQHVS"
     gene            1225..2247
                     /locus_tag="ACIE68_004759"
     CDS             1225..2247
                     /locus_tag="ACIE68_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HFX5910920.1"
                     /translation="MKKIDISGSPFEIGLRLGELGREAWHQKLMLTSLWQTVMTLQAS
                     PETRLMRAEVQRHYPHIWQELEGLAQGLEATVDQVFAWNCRGDLVRSTSDGCTTVAGV
                     NAAGERIIAHNEDGFPQLRDECLLATITPSRGLSFTSFAYPGSICGHTFAVNEKGVVN
                     TVNNIRAVHRPQGLPRQILARASLNANTLDEAITILTGQPRSGAFHHTLGQLGDTRLF
                     SVEATGQGCSVLPLTTVTGHANHLVHAALAGVEQIVTDSSASRQLRLVQWRETQPPFD
                     AAAAKAILSDTHDAELPIYRLAADDPDEENTLATAVFTLDANHVRWQIFDINRDDAKF
                     QGEVRG"
     gene            complement(2390..3205)
                     /locus_tag="ACIE68_004760"
     CDS             complement(2390..3205)
                     /locus_tag="ACIE68_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HFX5910921.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
BASE COUNT          791 a          836 c          882 g          811 t
ORIGIN      
        1 gaattgattc gcggtacgcc aattatggta caggtgatgt ttatctattt tgccttgcca
       61 atgattctgc ctgttcgtat cgaccctatc acggccgcga tggtcaccat tattattaac
      121 tccggcgcgt atattgcgga aattacgcgc ggcgcaattt tgtccatcaa tagaggattt
      181 aaagaagcca gtctggcgat gggattatcg cagcgcgcaa cgctatggca tgtgattatg
      241 ccgctggccc tgcgccgcat gatcccggcg ttgggcaacc agtggattat cagcattaaa
      301 gacacctcgc tgttcattgt tatcggtgtc gcggaattaa cgcggcaggg gcaggagatc
      361 atcgccggga acttccgtgc ccttgaggtc tggacagcgg tagccctgat ttatcttgcc
      421 gtcaccctgt gcctgagctt tctgctgaag caactggaga aaaggatcca tattttatga
      481 gcatggttga atttagcgcg gtcgctaaac atttcggcac cacccaggtg ctgtacgata
      541 tcaatctgaa aattgatgcc ggtgaagtgg tcgtgattat cggtccttca ggttcaggta
      601 aatcgacgct gctgcgttgt atcaacaagc tggaagagat ctcctctggc actttgttgg
      661 ttgccggaat gcacctgacc gatcctcacg taaacgagtg cgaaatccgc cgcgaagcgg
      721 ggatggtatt ccagcagttt catctgtttc cgcatctgac ggcgctggaa aatgtgatgt
      781 ttgggcctat tcgcgtacgc aaaatgagtc aagcggcagc ccgcgaacag gcgctggcgc
      841 tgctggaacg tgtcggcctg ggcgaacggg ccgagcacta tccgtcggag ctttcaggcg
      901 gccagcagca gcgcgtggct atcgcccgcg cgctggcggt gaaaccgaaa atgatgctgt
      961 ttgatgagcc aacctctgcg ctcgatccgg agttgcgcca tgaagtatta aaagtgatgc
     1021 gttcactggc ggatgaaggg atgacgatgg ttatagtgac gcacgagatt ggctttgcac
     1081 gcgatgtggc ctctcgattg atttttatcg atggtggaac cattgctgaa gatggcccac
     1141 ctgatatcct gctgaatgcc agtagcaatc cgcgcctgaa agaattttta cagcacgttt
     1201 cctgaacata agtgaatcca tcacatgaag aaaatcgaca tcagtggttc cccgtttgag
     1261 attggcctgc gccttggcga gcttggccgt gaagcatggc atcagaaact gatgctcacg
     1321 tcattgtggc aaacggtgat gaccctgcag gcatcccctg agacccggtt aatgcgcgct
     1381 gaggtgcagc ggcattatcc gcacatctgg caggagctgg aggggttggc gcaggggctg
     1441 gaggctactg ttgatcaggt ctttgcctgg aactgccgtg gcgatctggt gcgctcaacg
     1501 tcggatggct gtacgacggt ggccggggta aacgccgcag gcgagcggat tatcgcgcac
     1561 aatgaagacg gttttcctca gttacgggat gaatgcttgc tggctaccat taccccgagt
     1621 cgcggtctga gctttacgag ttttgcctat ccggggtcga tttgcggcca cacctttgcg
     1681 gtgaatgaaa aaggtgtcgt taacacggtg aataacatcc gtgcagtgca tcgtccgcag
     1741 ggattaccgc gtcagatcct ggctcgcgcc tcgctgaatg cgaacaccct cgacgaggcc
     1801 atcactatac ttaccggcca gccgcgatcc ggggcgtttc accatacgct cgggcagctg
     1861 ggcgatacgc ggctgttcag cgttgaggcc accggtcaag gctgttccgt tctccccctg
     1921 acgacggtaa ccgggcatgc caaccatctg gtgcatgcgg cactggctgg cgttgagcag
     1981 atcgttaccg acagttcagc gtcgcggcag ttacgcctcg tgcagtggcg ggaaacgcaa
     2041 cctccgtttg acgccgcggc ggcaaaagcc attctctcag atacccatga cgccgaactg
     2101 ccaatttacc ggctggctgc cgacgatccg gatgaggaga acaccctcgc cacggcagtt
     2161 ttcaccctcg atgccaacca cgtcaggtgg caaattttcg acattaaccg cgacgatgct
     2221 aaatttcagg gagaagtgcg tgggtgagat agccggtcgt cagtcataaa gggcagggta
     2281 acggcgttcg atagtaccgt ccggatactg cccgatatag gcgtcttgag cgatgtcctg
     2341 aagcgcggtg tccggaattt caggtttgtg tctctacaaa gactaactat cagaaaaact
     2401 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
     2461 gaaaaagccg tttctgtaat gaaggagaaa attcaccgag gcagttccat aggatggcaa
     2521 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     2581 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     2641 agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     2701 cgtcatcaaa atcactcgca tcaaccaaac cgttgttcat tcgtgattgc gcctgagcga
     2761 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     2821 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     2881 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     2941 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     3001 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     3061 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     3121 cccatttata cccatataaa tcagcatcca ggttggaatt taatcgcggc ctcgagcaag
     3181 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     3241 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
     3301 acacaacgtg gttcccgcca
//
LOCUS       DBANUU010000280         3305 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000280 DBANUU010000000
VERSION     DBANUU010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.286"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            166..1687
                     /locus_tag="ACIE68_004761"
                     /pseudo
     CDS             166..1687
                     /locus_tag="ACIE68_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(1712..2050)
                     /gene="maoP"
                     /locus_tag="ACIE68_004762"
     CDS             complement(1712..2050)
                     /gene="maoP"
                     /locus_tag="ACIE68_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFX5910922.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2169..3008
                     /gene="hdfR"
                     /locus_tag="ACIE68_004763"
     CDS             2169..3008
                     /gene="hdfR"
                     /locus_tag="ACIE68_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFX5910923.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(3104..3179)
                     /locus_tag="ACIE68_004764"
     tRNA            complement(3104..3179)
                     /locus_tag="ACIE68_004764"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3144..3146),aa:Trp,seq:cca)
     gene            complement(3188..3264)
                     /locus_tag="ACIE68_004765"
     tRNA            complement(3188..3264)
                     /locus_tag="ACIE68_004765"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3228..3230),aa:Asp,seq:gtc)
BASE COUNT          885 a          814 c          812 g          794 t
ORIGIN      
        1 tcgtagttca tcctttgtcc gctgcatttt tgcgagcatc attccaccgc cagaacaact
       61 caacttcact tgcacaaata aacaggaatc agccactaaa attcacgaat tagcgggcta
      121 cagcagcgat atcttatgca cataatggtg actcaaggag ggcttatgtc actgtcaatt
      181 gttcataccc gcgcagccct gggagtaaat gcgcccccga tcactgttga ggtacatatc
      241 agtaaaggtc tacccggctt aacgatggtg ggcttaccag aaacaacggt aaaagaagct
      301 cgcgatcgcg tgcgcagcgc cattatcaat agcggatatg aatatccggc gaaaaaaatc
      361 accatcaacc tggctccagc cgatctgcca aaagaagggg gacgatatga tttacctatc
      421 gccattgcgt tgctggcggc ctcagaacag cttacagcca ataagttaga atgaatatga
      481 attagtcgga gaactggctc ttacaggcgc tctgcgtggc gttcccggtg caatctccag
      541 tgcaactgaa gctattaagt cgggcagaaa aattatcgtc gcgaaagata acgaagatga
      601 agtggggcta attaacggtg aaggatgcct gatagccgat catctgcaag ctgtctgtgc
      661 gtttctggaa ggtaagcacg ctctcgaacg cccgaaacca actgatgcag tatcccgggc
      721 gctacaacat gatctcagtg atgttgtcgg tcaggaacaa ggaaagcgag gactggaaat
      781 taccgccgct ggcgggcaca accttttact gattgggccg ccgggaacag gtaaaacaat
      841 gctcgccagc cgtattaatg gcctgttgcc agatttaagc aatgaagagg cactggagag
      901 cgctgcgata ttaagtctgg taaatgctga atcagtacaa aaacaatggc ggcagcgccc
      961 gttccgctca cctcatcaca gtgcgtcgtt aactgcgatg gtgggcggtg gtgcaattcc
     1021 agggccaggt gaaatttcgt tggcgcataa cggcgtgctt tttcttgatg agctacctga
     1081 atttgaacgg cgtacactgg atgccttgcg agagccgatt gaatccgggc agatccatct
     1141 ttcacgcaca cgagcaaaaa taacctatcc agcccgtttc cagcttgttg cggcgatgaa
     1201 tcccagccct accggacatt atcagggaaa ccataaccgc tgcacgccag aacagacatt
     1261 acgttatctc aaccggctct cggggccctt tctcgaccgc ttcgatctct cactggagat
     1321 cccattacca ccccccggca ttttgagtaa aacggtagtg ccgggagaaa gcagcgccac
     1381 cgttaaacaa cgcgtaatgg ccgccagaga gcgccaattt aagcggcaga ataaactgaa
     1441 tgcctggctg gatagtccgg aaatacgcca attctgcaaa cttgagagcg aagatgcgat
     1501 gtggttggaa ggaacactga tccatctggg gttatcgatt cgtgcctggc agcggttatt
     1561 gaaagttgca cgaaccattg ctgatattga tcagtctgac attatcacac gtcagcattt
     1621 gcaggaggca gttagctatc gtgcgattga ccgtttgctc atccatctgc aaaaactact
     1681 gacataaaaa aagggcattt cgcccttttt attaatcatc agaatcggtg tagtcttcag
     1741 caccttcaac ctgcggttta ccgccggaaa gggtgtgaaa acgttttgga cgcttaatac
     1801 gcgtcatata cttggaccac acgcgttctg cttctgtcac tggctcacgt tcgccacggc
     1861 atactgctac gaagagtttc tcttcctcgg taaccggctc gcgtttgcca agatccaact
     1921 cattgaaggc ataaccatga cgctcaagca gttgtgcctc tttgatggtg aaatcaccat
     1981 gacgagagaa tccacgtgga taatgtttat tgtcgaaata tcgattagtc gtcgtaaagc
     2041 tttccgccat cctgcacgct cctaattctt tgaccgagct agttatggcg cggagtatta
     2101 gttacgcttg acagagtgta aaacaaaaca tttaaatcat aacgacaaat aattttgcgg
     2161 agagcactgt ggatacggaa ttgttaaaaa ctttcctgga agttagccga acgcgtcact
     2221 ttggtcgagc ggctgaatcg ctctatctga cccagtcagc agtgagcttt cgaatcagac
     2281 aactggaaaa tcaactgggt gtgaaccttt tcacccgcca cagaaacaat atccgtttaa
     2341 ccgctgccgg tgaaaaacta ctgccttatg cagaaacgct catgagcacc tggcaggccg
     2401 cccgtaagga ggtggcgcat acctcacgac ataacgagtt ttctatcggt gccagcgcct
     2461 cgttgtggga atgtatgctt aatcagtggc tgggacgctt gtatcaaaat caggatgccc
     2521 atacaggctt acagttcgaa gcgcgaattg cccaacggca gtctctggta aaacagctgc
     2581 atgaacgcca gcttgatctt cttattacca ctgaagcgcc caaaatggac gaatttagta
     2641 gtcagttgct gggatatttc actttagcgc tttataccag tgccccttca aaactaaagg
     2701 gagatcttaa ttatctgcga cttgagtggg ggccagattt tcaacagcat gaggcaggtt
     2761 tgatcggtgc tgacgaagtg cccattctga caaccagttc tgctgaactg gcacagcaac
     2821 agattgcgat gcttaatggt tgcacctggc tacccgtcag ctgggcgcgt aaaaaaggcg
     2881 gcctgcatac cgttgtcgat agcacaacac tttcacggcc gctttatgcc atatggctgc
     2941 aaaatagcga taaaaatacg ttgattcgcg atcttttgaa aattaacgtg ctggatgaag
     3001 tgtattaata tgaatggctg gcaaggatgc cggtagaagg atttacttcg gagagggtta
     3061 tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggcagg ggcggagaga
     3121 ctcgaactcc caacacccgg ttttggagac cggtgctcta ccaattgaac tacgccccta
     3181 attagggtgg cggaacggac gggactcgaa cccgcgaccc cctgcgtgac aggcaggtat
     3241 tctaaccgac tgaactaccg ctccaccgaa ttcttttaca accaccggtt ttatgaccgg
     3301 cttac
//
LOCUS       DBANUU010000281         3167 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000281 DBANUU010000000
VERSION     DBANUU010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.287"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            372..1862
                     /locus_tag="ACIE68_004766"
     CDS             372..1862
                     /locus_tag="ACIE68_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFX5910924.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTSWKNKEEILKAQKKALLSKLNKELRKGADESETLRQLEALQKNREEKPVRYKFI
                     FNDATTAAIKDQLCGQWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGGSLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLDKLNACYSQKLIRWHEQSFGL"
     gene            complement(1984..2556)
                     /locus_tag="ACIE68_004767"
     CDS             complement(1984..2556)
                     /locus_tag="ACIE68_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910925.1"
                     /translation="MQKLENRCELLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2642..2848)
                     /locus_tag="ACIE68_004768"
     CDS             complement(2642..2848)
                     /locus_tag="ACIE68_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFX5910926.1"
                     /translation="MNTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVETWLQARIAQSRP"
BASE COUNT          827 a          701 c          815 g          824 t
ORIGIN      
        1 agtactcagc attcagcgca ggaactgtca tcttctttat tttattctcc ttcaggaggg
       61 cagttttctg aaagcgttac tccggggaaa ctgtttatgt ttattcccgg caggaaaccc
      121 gtacctgcaa taccctggat gatgcttttt gctggtacgt tgttgtgtga aaattaatca
      181 acatgtgtgc tccggagagc gcccgtagac gcacagattc attctgacat tctttagcga
      241 caaaataccg agtggttatt atttaacctg aaaatcgaca gaaattttta tcctgtattg
      301 attctgctgc tatttttgct attagtagca atagttgcgg tactttatgt tctgtgtgtt
      361 gtgaggtcat tatgccatac tttcagtatc ctgatgagtt tcccctgagc tcgctgcctc
      421 cactcattcg tgatgccgtt attgaagctc agcaaatcac gcaggctccc ctggggcttg
      481 tggcagcatc tgccctgggc gctgtttcac ttgtctgcca gaatctgatt gatgtctgca
      541 gactgaatac actacggggg ccggtttctc ttttcttact gacgcttgct gagtccggag
      601 aacgaaaaac cgcagtggac aaattattaa tggagcccct gtaccagcag gagatgctgt
      661 tatattccag acacaaaaat gaactgacgt catggaaaaa taaggaagag attttaaagg
      721 cgcagaaaaa agcattgttg tcaaaactga ataaagaact ccgcaaaggc gcggatgagt
      781 cagaaacgct cagacaactg gaagcgcttc agaaaaacag agaggaaaaa cctgtaagat
      841 ataaattcat ttttaatgat gccacgacag cagcaattaa agatcagctt tgtggtcagt
      901 ggcgttcagt gggtatcatg tctgatgaag ccggtataat atttgatggg tataccttgt
      961 ctgagctgcc ttttattaac aaaatgtggg atggctccgt attgtcggtg gacagaaaga
     1021 atgaacccga acagatgatt gaaaatgcca gaatgacatt atccctcatg gtacaacccg
     1081 gtctttttga ccgatatatg gagcgaaaag gttctgttgc cagggacagt ggttttcttg
     1141 cccgttgtct aatcagtaaa cctgccacca cgcaggggaa acgctttatt aacggagctg
     1201 tgaccccggg aggatcatta acggcatttc atgaacgatt aatggaactg gccagaggca
     1261 gtattgaaaa atccagtaag gatgaacgtt attgtctgca tttttctcct gaagcgcaaa
     1321 aaatatttat tgatcactat aatgttctgg agcaggattt gtctccttcc ggacccttaa
     1381 gccagtttcg ggggcatgtt tcaaaaaaaa cagaaaacat tgcgagaatt gctgcgttat
     1441 tgcagtattt cagtcagggt gaagggaaaa tctctgccga tatcatgacg tctgctgtcg
     1501 taataagttc gtggtacaca gatgaatata aaaaattatt tgcgttacct gatgaatctg
     1561 aactgcagct gcaggacgcc cgggagctgc tcgactggct cattgaggag tgccgcggag
     1621 aatgcccgcc ccgtgtcagg aaaaattata ttctccagtg tggcccgggc cggttcagga
     1681 acaggaaaaa gctgaacgtc ctgctgaaca ttcttgcatc tcaactcagg ctctctgtcg
     1741 tgcaggaagg aaaaaccacc tatgtcctgc tttccgaaat aaacaatatc acgctggata
     1801 aactgaatgc gtgctacagt cagaaactga taagatggca tgagcaatcc tttggcttat
     1861 aatgttccct gttgctgaga cggattattc agtggtggta tcgcagtatg cccgtcaccc
     1921 cccgcttctg catgagggtg acggaggaaa agtaagtgtg cggaggcccg ggagcgttat
     1981 gaatcaacag gcgtataaca ggtactcgtc aatcatcagt tccagctcgc gcttttcccg
     2041 tttgcgcgcc acagcccagt tttcaagctc tgcatgtgat ttatgcttta actcttcagt
     2101 ccagggctgc atggagtggg cacacatcag atcataccag cacagccata tcagcgtgtg
     2161 gtagagatca cgatcgtcga aacgccggag caggctgttc agcctgtttg ccagccggtt
     2221 ttcacccaga cgatgtagct cactgagtaa aacgccgccc agattaaaat gtgtaagcag
     2281 catgatttca tgggtctgaa tgctggtcat taacccttcc tgaatggcag gaatgaacgg
     2341 tggtgttccc gggttgacga cctgatagag tccctggggg gccaccgaaa tgatggagcc
     2401 tttttccggc agaaaaaagt gtgccagggt cagacggtga taaccatgaa gatgcggaga
     2461 ccccattcca tgagcgacga taagcctcgt cacgcttgtc atccatttct gatgctggat
     2521 gagcagcagc tcacaccggt tttcaagttt ttgcataata ttctctcctt tagcgcgcag
     2581 caaaaggcct ccgggggccg gatcatgcct ttgctgcggt tgaacggata acggtcagga
     2641 attacggacg ggactgtgca atacgcgcct gcagccaggt ttccacttca cttttcagcc
     2701 agcgggaact gcggcccagt ttgatggggg caggaaaggc accatccttg atgagcttgt
     2761 aaaaccattt atcggtcagg ccggtcagtt gagtgataaa tgccatgtcg accatctggt
     2821 catccatcag tgaaactggg gtattcatga aaatgttcct ccggatgttg ttaatctcaa
     2881 ccggcgttgg tgatgaaaac aacgcaccac cttcaccggc ataacaaccg gggagaaacg
     2941 taaaaaataa tcgggaatgt gaccgtcagg agataaacgg ggggattgcg gtggctgagc
     3001 cgtaccggta tgtcgggtga ttgttgcgaa acgatgcttc cgcacgcgca cgggaggact
     3061 ttcgtgagac aaggggaaaa ctggcgttct gataattgca gagttgtttt tgaaggcggt
     3121 tgtggtgcat cagcctgtga cctgtgggca ggcgacaggt atcactg
//
LOCUS       DBANUU010000282         3160 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000282 DBANUU010000000
VERSION     DBANUU010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.288"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(60..587)
                     /gene="mobB"
                     /locus_tag="ACIE68_004769"
     CDS             complement(60..587)
                     /gene="mobB"
                     /locus_tag="ACIE68_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFX5910927.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(569..1153)
                     /gene="mobA"
                     /locus_tag="ACIE68_004770"
     CDS             complement(569..1153)
                     /gene="mobA"
                     /locus_tag="ACIE68_004770"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFX5910928.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMVF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1223..1492
                     /locus_tag="ACIE68_004771"
     CDS             1223..1492
                     /locus_tag="ACIE68_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFX5910929.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1569..2555
                     /gene="srkA"
                     /locus_tag="ACIE68_004772"
     CDS             1569..2555
                     /gene="srkA"
                     /locus_tag="ACIE68_004772"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFX5910930.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLMPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2572..>3160
                     /gene="dsbA"
                     /locus_tag="ACIE68_004773"
     CDS             2572..>3160
                     /gene="dsbA"
                     /locus_tag="ACIE68_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFX5910931.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQ"
BASE COUNT          795 a          768 c          774 g          823 t
ORIGIN      
        1 ttggcgcaga aaaaaatgcc ggatgcgacg ctggcgcgtc ttatccagcc tactcttgct
       61 tatccgttct gcttttgcat ccactccacc acaaaatcag ccagcccctc aacatcatta
      121 atatccagta atgcgacatc aagattaagc ggcacatcac tggctacagc aataacatgc
      181 ctgtctatca ctaattcttc aggtcgatgt ccggctccat cgcgaaacag cacaatcttt
      241 gcgatctctt catgcttaaa cccttcgacc agaatcaaat ccagctttga ggtatccatt
      301 cgacttgcga gaaactgtag atccagctct tcttcgtctg gtgtttccgt catcaaggcc
      361 catcgttgct ggctggcaac gatggtttgt gccgcgccag ccttgcgcag ctcatagcta
      421 tctttgcctg gcttatcaac atccatatca tggtgcgtat gcttaatcag ccctggacgg
      481 atccctctgg cgcataatgc cgggatcagt tttttcaaaa gcgtagtttt tccggtacca
      541 ctccacgcgg caaaggcgag taacggtatc atcgtttttc ctgccatcgg gcaagctcct
      601 ctggcgtatt cacgttaaca aatgcatctt tatgatcgct gaaatcaacc gcatgaccgc
      661 cagccagacg cataaatacc attacccggc gttctcctgc ttgcagatat tccagtaata
      721 aaggctcaat agcgcggttt accagagcaa tagtcgggtg atcgcgttca ccgtcatgga
      781 cccacacgac aggcgcatct ttgcgctgat gattaagcct ggctgctaaa tcagggggaa
      841 tgtaaggcgt atcgcacggg caaaacaaaa accactcacc agcttcctgc tgcattactg
      901 aaagcattcc tgccagaggg cctgggtaat ccgccagtga atcttcaatc actttcagac
      961 cgcttgcctg atagatttcc tgatgacgat tagcattaac cacgacgtga gagagctgcg
     1021 tcataagcgc gtcagcgaca tgttgccata atggtttgcc gtttaattca agcaatcctt
     1081 tatctacgcc gcccattcgt ctggctttac cgcctgccag cacaacgcct gttatcgtcg
     1141 tcatcagatt caccgatatc gcctctttta ttgtgggatt gaccctgcta acgtgtctgt
     1201 ctcaagagta aggagcatca ctatgaaatg taaacgtctg aatgaagtta ttgaactcct
     1261 ccagccagcc tggcaaaaag agccagacct taacctgctg caatttttgc agaaactggc
     1321 gaaagagtca ggttttgacg gcgaactggc ggatttgacg gatgacattc tgatctatca
     1381 cctgaaaatg cgtgattccg cgaaagatgc ggtgatcccg ggtttgcaga aagattatga
     1441 agaagacttc aaaacggcgc tgttacgcgc tcgcggcgta attaaagagt aaaagcttgt
     1501 aagcggcgcc accaaaatca tcgtgaaatg atatccttcg tcattcgtaa tgttttccgg
     1561 atgatgggat gaataacagc gcttttactt tccagacact acacccggat accatcatgg
     1621 acgctctgtt tgagcatggg atccgggtgg attccggtct taccccgctt aacagctatg
     1681 aaaaccgtgt ctatcaattt caggacgaag atcgtcgacg ttttgtcgtc aaattttatc
     1741 gccctgaacg ttggacagcc gatcaaatcc tcgaagaaca tcaatttgcg ttgcagctgg
     1801 taaatgatga agttccggtc gcagcacctg tggcctttaa cggtcagact ttattgaatc
     1861 atcagggatt ttatttcgct gtttttccaa gcgtcggtgg tcgccagttc gaagctgata
     1921 atatcgatca gatggaagcg gttgggcgtt atttagggcg tatgcaccag acggggcgca
     1981 aacagctttt tatccatcgc ccgaccatcg gtctgaatga atatctcatt gagccacgca
     2041 agctgtttga ggacgctaca ctgatgcctt ccgggttgaa agcggcattc ctgaaagcga
     2101 cagatgagct gatcgccgcc gttacagcac actggcggga agacttcacc gttctgcggc
     2161 tacatggaga ctgccacgcc gggaatattc tctggcgcga tggtccaatg tttgttgatc
     2221 tggatgatgc acgtaatggt ccagctattc aggatttgtg gatgttgctc aatggcgata
     2281 aagccgagca gcggatgcaa ctggaaacta ttattgaagc ttatgaagaa tttagcgagt
     2341 tcgacaccgc tgaaatcgga ctgattgaac ctttacgcgc catgcgtttg gtttattatc
     2401 ttgcctggct aatgcggcgt tgggctgatc ccgcgttccc gaaaaatttc ccgtggttaa
     2461 ccggggaaga ttactggctg cgacaaacgg cgacttttat agaacaggca aaagttctac
     2521 aagaaccccc tttacaatta acacctatgt attaatcgga gagagtagat catgaaaaag
     2581 atttggctgg cgctggctgg tttagtttta gcgtttagcg catcggcggc gcagtatgaa
     2641 gatggtaaac agtacactac cctggaaaaa ccggtagctg gcgcgccgca agtgctggag
     2701 tttttctctt tcttctgccc gcactgctat cagtttgaag aagttctgca tatttctgat
     2761 aatgtgaaga aaaaactgcc ggaaggcgtg aagatgacta aataccacgt caacttcatg
     2821 ggtggtgacc tgggcaaaga tctgactcag gcatgggctg tggcgatggc gctgggcgtg
     2881 gaagacaaag tgactgttcc gctgtttgaa ggcgtacaga aaacccagac cattcgttca
     2941 gcatctgata tccgcgatgt atttatcaac gcaggtatta aaggtgaaga gtacgacgcg
     3001 gcgtggaaca gcttcgtggt gaaatctctg gtcgctcagc aggaaaaagc tgcagctgac
     3061 gtgcaattgc gtggtgttcc ggcgatgttt gttaacggta aatatcagct gaatccgcag
     3121 ggcatggata ccagcaatat ggatgttttt gttcagcagt
//
LOCUS       DBANUU010000283         3149 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000283 DBANUU010000000
VERSION     DBANUU010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.289"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            102..1784
                     /gene="ydeN"
                     /locus_tag="ACIE68_004774"
     CDS             102..1784
                     /gene="ydeN"
                     /locus_tag="ACIE68_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFX5910932.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1836..2993
                     /gene="ydeM"
                     /locus_tag="ACIE68_004775"
     CDS             1836..2993
                     /gene="ydeM"
                     /locus_tag="ACIE68_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFX5910933.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CSFLKEHEFLAGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2959..3081)
                     /locus_tag="ACIE68_004776"
     CDS             complement(2959..3081)
                     /locus_tag="ACIE68_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910934.1"
                     /translation="MTTHPTGIYVGCFVKVIRRRLRLELRGRVINYSPFVLQHP"
BASE COUNT          959 a          684 c          634 g          872 t
ORIGIN      
        1 ctaaatctgc tatctcttta tgtgactaac ttcacttaca cccacttatt tctcttcgta
       61 aaattacttc ggaattaagt acaataagaa gaggaacatt tatgaagtct gcattaaaga
      121 aaagtgtcgt aagtacctcg atatctttga tactggcatc tggtatggct gcatttgctg
      181 ctcatgcggc agatgatgta aagctgaaag caaccaaaac aaacgttgct ttctcagact
      241 ttacgccgac tgaatacagt accaaaggaa agccaaatat tatcgtacta accatggatg
      301 atcttggtta tggacaactt ccttttgata agggatcttt tgacccaaaa acaatggaaa
      361 atcgtgaagt tgtcgatacc tacaaaatag ggatagataa agccattgaa gctgcacaaa
      421 aatcaacgcc gacgctcctt tcattaatgg atgaaggcgt acggtttact aacggctatg
      481 tggcacacgg tgtttccggc ccctcccgtg ccgcaataat gaccggtcga gctcccgccc
      541 gctttggtgt ctattccaat accgatgctc aggatggtat tccgctaaca gaaactttct
      601 tgcctgaatt attccagaat catggttatt acactgcagc agtaggtaaa tggcacttgt
      661 caaaaatcag taatgtgccg gtaccggaag ataaacaaac gcgtgactat catgacaact
      721 tcaccacatt ttctgcggaa gaatggcaac cgcaaaaccg tggctttgat tactttatgg
      781 gattccacgc tgcaggaacg gcatattaca actccccttc actgttcaaa aatcgtgaac
      841 gtgtccccgc aaaaggttat atcagcgatc agttaaccga tgaggcaatt ggcgttgttg
      901 atcgtgccaa aacacttgac cagcctttta tgctttacct ggcttataat gctccgcacc
      961 tgccaaatga taatcctgca ccggatcaat atcagaagca atttaatacc ggtagtcaaa
     1021 cagcagataa ctactacgct tccgtttatt ctgttgatca gggtgtaaaa cgcattctcg
     1081 aacaactgaa gaaaaacgga cagtatgaca atacaattat tctctttacc tccgataatg
     1141 gtgcggttat cgatggtcct ctgccgctga acggggcgca aaaaggctat aagagtcaga
     1201 cctatcctgg cggtactcac accccaatgt ttatgtggtg gaaaggaaaa cttcaacccg
     1261 gtaattatga caagctgatt tccgcaatgg atttctaccc gacagctctt gatgcagccg
     1321 atatcagcat tccaaaagac cttaagctgg atggcgtttc cttgctgccc tggttgcaag
     1381 ataagaaaca aggcgagcca cataaaaatc tgacctggat aacctcttat tctcactggt
     1441 ttgacgagga aaatattcca ttctgggata attaccacaa atttgttcgc catcagtcag
     1501 acgattaccc gcataacccc aacactgagg acttaagcca attctcttat acggtgagaa
     1561 ataacgatta ttcgcttgtc tatacagtag aaaacaatca gttaggtctc tacaaactga
     1621 cggatctaca gcaaaaagat aaccttgccg ccgccaatcc gcaggtcgtt aaagagatgc
     1681 aaggcgtggt aagagagttt atcgacagca gccagccacc gcttagcgag gtaaatcagg
     1741 agaagtttaa caatatcaag aaagcactaa gcgaagcgaa ataactaaac cttcatgcgg
     1801 cggatttttc cgccgcctta ttgagcgaga tagcgatgca cgttacagcc aagccctcca
     1861 gttttcaatg taatctcaaa tgtgattact gtttttacct tgaaaaagag tcgcagttta
     1921 ctcatgaaaa atggatggat gacagcactc tgaaagagtt catcaaacaa tatattgcag
     1981 cgtctggcaa tcaggtctat tttacctggc aaggcggtga acccactctg gctggcctgg
     2041 attttttccg taaagttatt cactatcaac aacgctatgc aggccaaaaa cgtattttta
     2101 atgcattaca aacgaatggc attttattga ataatgaatg gtgttccttt ctcaaagaac
     2161 atgaatttct ggctggtatc tcgatcgatg gcccccagga gttacatgac cgttacagac
     2221 gcagtaattc aggtaacggt acttttgcaa aagtgatagc agccatcgag cgtctgaaat
     2281 catatcaagt agagtttaat acgttaaccg tcattaataa cgttaatgtc cattaccctc
     2341 ttgaggttta tcatttttta aaatctatcg gcagtaaaca tatgcaattt atcgaattgc
     2401 tagaaaccgg gacgccgaat attgatttca gtggtcatag tgagaacaca ttccgtatca
     2461 ttgatttttc tgtgcctccc acggcttatg gcaagtttat gtcaaccatt tttatgcaat
     2521 gggttaaaaa cgatgtgggt gaaattttca ttcgtcagtt tgaaagcttt gtcagccgtt
     2581 ttttggggaa tgggcataca agttgtattt tccaagagtc ctgcaaggat aatctggttg
     2641 ttgaaagtaa tggagacatt tacgaatgcg accattttgt ctatccacag tacaaaattg
     2701 gaaacattaa taaatctgaa ctcaaaacga tgaacagtgt acaactgaca gcgcaaaaaa
     2761 aacggatttc agcgaaatgt cagcaatgtg catataaacc tatctgcaat ggcggttgtc
     2821 ctaagcatcg tattactaaa gtaaacaatg agactgtttc ttatttttgc gaaggttata
     2881 aaatcctttt ttcaaccatg gtaccttata tgaacgccat ggtagagtta gctaagaaca
     2941 gagtaccgct ttatcacatt atggatgttg caaaacaaat ggagaataat taataacacg
     3001 ccctcttagc tccagtctta gcctccgtct tatgacctta acaaagcacc cgacataaat
     3061 tcctgtcggg tgtgtcgtca tcatcatctt cccttgcgaa aatcctggca tacgtggtat
     3121 gcgtcagggc attagcaata cccttgatt
//
LOCUS       DBANUU010000284         3125 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000284 DBANUU010000000
VERSION     DBANUU010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.290"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..2721
                     /gene="traG"
                     /locus_tag="ACIE68_004777"
     CDS             <1..2721
                     /gene="traG"
                     /locus_tag="ACIE68_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFX5910935.1"
                     /translation="ALTLSVVAVAFMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQI
                     IDNSDLVKVHRVDNVPVGLAMPLSLTTRIGHAMVVSYEMIFTQPDSVTYSKTGMLFGA
                     NLIVKSTDFLSRNPEIINLFQDYVQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPS
                     PLRGVYDNNNNFITCKDASVTLKDRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQL
                     VSDSYSYFYGSSQSASQIMRQNVTINALKEGITSNAARNGDTASLVNLATTSSMEKQR
                     LAHVSIGHVTMRNLPMVQTILTGIAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQT
                     WPLLYAILNSAMTFYAKMNGAPVVLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVK
                     GLGAGFSSVYSHFASSSISPTASAAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQ
                     MMYQTGSGATATQTRDGNMVMDASGAMSRLPVGINATRQIAAAQQEMAREASNRAESA
                     LHGFSSSIASAWNTLSQFGSNRGSSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAH
                     NISNEQATQELASTGSKFSYGGYADAHAEWGIKPGILGVGGGVGVRGGFKGSLDRTDD
                     DSHSASSGSRATHDARHDIDAKASKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLS
                     AALNSAKQSYDQYTTNMTRSHEYAEMASRTESMSGQMSEDLSQQFAQYVMKRAPQDAE
                     AILTNTSSPEIAERRRAMAWSFVQEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDI
                     IADHQGHQAIIEQRTQDSNIRNDVKHQVDNMVTEYRGNIGDTQNSIRGEENIVRGQYS
                     ELQNHHKTEALSQNNKYNEERESQKIIPEPSEEAVKKMVDDKKDRLKGPL"
     gene            2718..>3125
                     /gene="traS"
                     /locus_tag="ACIE68_004778"
     CDS             2718..>3125
                     /gene="traS"
                     /locus_tag="ACIE68_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628097.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HFX5910936.1"
                     /translation="MITHYDIKTETQKLKDVLSVEGVNIPPLLQVIKPGVYVFLWVLL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPKKFRDESKVISF
                     MYDKNKTYILSFVIVFSIVSFAHTFLFGFLSITL"
BASE COUNT          820 a          688 c          828 g          789 t
ORIGIN      
        1 gcgctcaccc tgtctgttgt tgctgtggct tttatgtggg tacagcggca taacgtgatg
       61 gatttgctgg gctgggtggc cgtgtttgtg cttatcagcc tgctggttaa tgtccgcaca
      121 tcggtgcaga ttattgataa cagtgacctg gtcaaagtcc atcgggtgga taatgtgccg
      181 gtcggtctgg cgatgccact ttctcttacg acccgtatcg ggcatgcgat ggtggtcagc
      241 tacgagatga tattcaccca accggacagc gtcacctaca gcaagacagg catgctgttc
      301 ggggcgaatc tgattgtgaa aagcaccgat ttcctgtccc ggaatccgga aatcatcaat
      361 ctgttccagg actacgtcca gaactgcgtg ctgggtgata tttacctgaa tcataaatac
      421 acgctggaag atctgatggc ctccgctgac ccctacacgc tgattttttc ccgacccagc
      481 ccgctacggg gcgtttatga caacaataat aatttcataa cctgtaagga tgcgtcggtc
      541 acgctgaaag acaggctgaa tctcgataca aagacgggag gcaagacctg gcattattat
      601 gtgcagcaga tatttggcgg cagaccggac ccggacctgt tattcagaca actggtcagt
      661 gacagttaca gttatttcta cggctccagt cagtctgcca gccagattat gcgccagaac
      721 gtcacaataa atgccctgaa agagggtatc accagtaatg ccgcccgtaa tggtgacacc
      781 gccagcctgg tgaatctggc caccacgtca tcgatggaga agcaacgtct ggcgcatgtc
      841 tctatcggtc atgtgacaat gcgaaacctg ccaatggtac agactatcct gacgggaatt
      901 gccattggta ttttcccgct tctggtactg gcagctgtct tcaacaaact gacactgtcc
      961 gtcctgaaag gctatgtgtt tgccctgatg tggctgcaga cctggccgtt gttgtatgcc
     1021 attctgaaca gtgccatgac attctatgcg aagatgaatg gtgcgccggt cgtgctgtct
     1081 gaactctctc agatacagct gaaatactct gacttggcct ctactgccgg atatctttca
     1141 gccatgatcc ccccgttgtc atggatgatg gtaaaaggcc ttggcgcagg tttttccagc
     1201 gtgtacagcc acttcgcctc ttcctctatc agtccgacgg ccagtgcagc aggcagtgtg
     1261 gttgacggta attactccta cggcaacatg cagacggaaa acgtgaacgg cttcagctgg
     1321 agcaccaaca gcaccacgtc gtttggtcag atgatgtacc agaccggcag tggcgcaact
     1381 gccacacaga cccgtgacgg taatatggtg atggatgcaa gcggagcgat gtcccgttta
     1441 ccggttggta tcaatgcaac gcgtcagatt gcagcggcac aacaggaaat ggcccgggag
     1501 gcgtcgaaca gagcagaaag tgccctgcat ggtttcagca gcagtattgc cagtgcctgg
     1561 aacacgctca gtcagtttgg ttctaaccgg gggagcagtg attctgtcac cggtggcgct
     1621 gacagcacca tgagtgcaca ggactccatg atggccagcc gtatgcgcag tgcagtggaa
     1681 agctatgcga aggcgcataa tatcagtaat gagcaggcga ctcaggaact ggcatcaaca
     1741 ggttctaagt tctcgtatgg cggatatgca gatgctcatg ctgaatgggg gattaaacct
     1801 ggaattcttg gtgttggtgg tggggttggt gtcagaggcg ggtttaaggg aagtcttgat
     1861 cgaacggatg atgattcaca ttctgcaagt agtggcagca gagctaccca tgatgctcgc
     1921 catgatattg acgctaaagc cagtaaggac tttaaagaag ccagcgatta ctttaccagt
     1981 cgcaaggtca gtgagtccgg cagccatact gacaataatg ctgactcccg ggtggaccag
     2041 ttgtctgcag ccctgaactc agcgaaacag agttacgacc agtacacgac gaatatgacc
     2101 cgcagccatg aatatgcgga aatggcctcc cgtactgaaa gcatgagcgg gcagatgagt
     2161 gaagacctgt cgcaacagtt tgcacagtat gtgatgaaac gcgcaccgca ggatgcagaa
     2221 gctattctga caaataccag ttcgccggag attgcagaac gtcgtcgggc gatggcctgg
     2281 tcttttgtgc aggaacaggt acagcctggt gttgataatg cctggcggga atcccgtggc
     2341 gatattggta aaggaatgga gagcgtacct tcgggtggtg gcagtcagga tattattgct
     2401 gaccatcagg gacatcaggc cattattgag caaagaacgc aggacagtaa tatccgtaat
     2461 gatgtaaaac atcaggttga taatatggtc acagaatatc gtggcaatat cggagatact
     2521 cagaacagta tccgtggcga ggaaaatatt gtaagagggc agtattctga actgcaaaat
     2581 catcataaaa cagaggcatt atctcagaat aataaatata atgaagagcg agaaagtcaa
     2641 aagataatac ctgagccatc agaagaggct gtgaaaaaaa tggttgatga taaaaaggat
     2701 agactgaagg ggccattatg atcactcact atgatataaa aacggagaca cagaaactaa
     2761 aggatgtact ctctgtagag ggagttaata ttccgccatt attacaagtc ataaaaccag
     2821 gggtgtatgt gtttttatgg gttttattat ggccgacatt tcttcgtttg ctggcagata
     2881 aagttgatat ccgagatgct ggatttgata tatgttttag tggcgtaatg ggttttatcc
     2941 tttttgttgc cattactaat ggaatgatgt tgtatcttgc catccctaaa aaattcagag
     3001 atgagtcgaa agttatatct tttatgtatg ataagaacaa aacttatatc ttgtcgtttg
     3061 tgattgtttt ttcaatagtt tcctttgctc atacgttctt atttgggttt ttatcaataa
     3121 ctctg
//
LOCUS       DBANUU010000285         3068 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000285 DBANUU010000000
VERSION     DBANUU010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.291"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            1..1256
                     /gene="sctN"
                     /locus_tag="ACIE68_004779"
                     /pseudo
     CDS             1..1256
                     /gene="sctN"
                     /locus_tag="ACIE68_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
     gene            1246..1702
                     /locus_tag="ACIE68_004780"
                     /pseudo
     CDS             1246..1702
                     /locus_tag="ACIE68_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            1680..1909
                     /locus_tag="ACIE68_004781"
                     /pseudo
     CDS             1680..1909
                     /locus_tag="ACIE68_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            2206..2553
                     /locus_tag="ACIE68_004782"
     CDS             2206..2553
                     /locus_tag="ACIE68_004782"
                     /inference="COORDINATES: protein motif:HMM:NF014559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     determinant, SpaN/EivJ family"
                     /protein_id="HFX5910937.1"
                     /translation="MFTQHIEKQRNVNTLNQNDINNSVNNANVHENELTYQFQRWGQN
                     HTVRILESSEGIRLKPSDTLVSNRLREAQHNDVTAQRWVLTEQDERQGQRHQPYEEQE
                     NESKFENDQKDES"
     gene            2513..3068
                     /locus_tag="ACIE68_004783"
                     /pseudo
     CDS             2513..3068
                     /locus_tag="ACIE68_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT         1062 a          545 c          689 g          772 t
ORIGIN      
        1 tacatagcat aaacggctct ctgattgaag cagaacttga cgacgtttca gtgggtgaag
       61 tatgcgaaat ttatgcaagc tggcaagcaa atgaacgtat agccagggct caggtcgttg
      121 gctttcgcaa tggaaagaca ttattaaacc ttataggcag ctcagtgggg cttacacgta
      181 ctgcagtttt gaaaccaacg ggagagcagt taaccataca gatcagtgat gcctttcttt
      241 gttcggtttt gaatgcctct ggtcaaatta tggaaaggtt tgtcccgaac cctccaggcg
      301 atagaggaaa tttacgcctg attgatgaac tgccaccgtc gtatcaggaa cgtcgagtta
      361 ttaatacacc actagagaca cgcataagag tcattgatgg cgtactgaca tgtggcattg
      421 gtcaacgtgt tgggattttt gcatcggctg gctgtggcaa aacggtttta atgcatatgc
      481 tcgtaaacaa tactgaagct gatgtgtttg ttattggctt gattggcgaa cgtggtaggg
      541 aagatacgga atgcgcggaa tcgatgaaac aatcagtaaa tgcggcaaaa tgcgtattgg
      601 tttatgccac ctctgatttc tcgtcagtag atcggtgtaa tgccgccctg atggcgacaa
      661 ccgtggcaga gtattttaga gatcgaggaa agagagttgt actttttatc gactcaatga
      721 cgcggtatgc atgagcacta cgtgatatga aactggcagc gggtgaacca cctgccagag
      781 gtggttatcc tgcatctgtt tttgacagtt tgcctcgctt actggagagg ccgggaccaa
      841 cactaaaagg aagtatcacc gcgttttata ccgtgttact ggaaggggaa gacgaatcag
      901 accccctggg agatgaaatt cgctcaatcc tcgatggcca tatctatctg agtcgcaaac
      961 tcgctgggca aggtcactat ccggcaataa atgttctaaa aagtgtgagt cgagtatttg
     1021 gtcaggtcac tgatgaaaaa cacagagata atgctgcacg ggtaaggaaa atcctcacaa
     1081 ctttggaaga tctacaggtt tttattgact taggggaata tcgcgctggg caaaatgctg
     1141 aaaatgattt cgctatgaat gcacggccta aactcacaaa ttggttgaaa cagtcagtta
     1201 atgaaaaaat gccaatgagt gaaactctaa aagagcttga acgaattgtt aagtaaagtt
     1261 aatcgactta tcagaagaac agcgcagagc ctggctgcat gtgaagcatc attacaaaag
     1321 ttaaatgcag aaaaagaaaa gttagccgaa aaggaacgcc tgtatgatat gcaacttaaa
     1381 aacctgaagt cactgttaga taagaaggag ttactcggcg aagttgtatt tcgacaggat
     1441 atattttact cgttacgtaa agttgcagtc attcagcagc agatcgctga gataaatctt
     1501 gaaaagcaaa aaatagcaga gcggagaaaa atacttaaca aggaaatagt tcaacagcaa
     1561 gcacaaagaa aacactggtg gctaaaagga gaaaagtacg ttcgactaaa aacgcgcatc
     1621 aaaaaaactt ttaaatcaga tgcttcatca agacgagctt gaacaggagg aaaaatataa
     1681 tggacgaagt caagaaaatt gaacacggta aaattttaaa cacagacaca cccgataatt
     1741 ctgcttcgaa tttagacagt ttcttaaaga aaaacaaaaa aataaagaaa tgagtgagat
     1801 tgtcgctgga accattctcc agttattacg tcagactcag gatagccaat tttctacatt
     1861 ggggaaaaag aatgggcaaa tgaagtctat tgacaacaaa tcatctctcc aggttgacag
     1921 gaattcctct ctttctttgc aaacaaatgg aacagaacaa ttttctacaa agaaaattct
     1981 caatatgact tctcgagatc agggtattag ttctgaacta tctaataaaa cgatacaaat
     2041 taatgaaaaa atccataatg gtatccatac agaatacatc acagatcaaa aaacagtaat
     2101 aataaagatc gtgaaaaaaa ataccgcgat ggagacaaaa taaatggacc gcaggcccat
     2161 tcattagaca gtactaacga aagaaggttt gctgataaca gaaccatgtt tactcaacat
     2221 atcgaaaagc aaaggaatgt taatacgctc aatcagaatg atataaataa ttctgttaat
     2281 aatgccaatg tgcacgaaaa tgaattgaca tatcagtttc agcgttgggg acaaaatcat
     2341 acagtccgga ttctggaaag tagtgaaggg atacgactaa aaccatcaga cacacttgtc
     2401 tccaacagac tccgtgaagc ccaacacaat gatgttacag cacaacgttg ggtattgact
     2461 gagcaagatg aacgacaagg acaacgacat cagccgtatg aggagcaaga aaatgaaagc
     2521 aaattcgaaa acgatcagaa ggatgaaagt taatgttcgg tctgaggaaa gtaaatcgaa
     2581 acactcatat gtttgaaacc acttttcaaa attggaaaga aaatggcgaa gatgttgcat
     2641 tattaatgcc cgaatttagt gctaaatggc taccgatagc agaagagagt ggcagctggt
     2701 ctggatgggt attgcttgga gaaatatttc ccttaatttc atctgagctc gctgggatgg
     2761 ctttaatgcc agaaacggaa aggttaatag gggaatggct cagtttatca agttccccgt
     2821 taaatctaaa gcatcctgaa ctaaaatata atcgtttgcg cgtaggtaaa gtatttgatg
     2881 gagtattgag ccctgctcaa ccactaataa gaatatggac aggggaattg aatctttggt
     2941 tagataaagt cacagtctgt caatacggaa acgctccagc gttagacaaa aaatcattat
     3001 attggtccat tcattttgta attggattta gtaaaacatg ttacagaagt cttattgata
     3061 ttgaagtt
//
LOCUS       DBANUU010000286         3013 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000286 DBANUU010000000
VERSION     DBANUU010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.292"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1409)
                     /gene="traB"
                     /locus_tag="ACIE68_004784"
     CDS             complement(<1..1409)
                     /gene="traB"
                     /locus_tag="ACIE68_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFX5910938.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQTPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGG"
     gene            complement(1409..2137)
                     /gene="traK"
                     /locus_tag="ACIE68_004785"
     CDS             complement(1409..2137)
                     /gene="traK"
                     /locus_tag="ACIE68_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFX5910939.1"
                     /translation="MRKNNTAIIFGSLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQTPAGLSSVADAVWTGNHLKMIRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(2124..2690)
                     /gene="traE"
                     /locus_tag="ACIE68_004786"
     CDS             complement(2124..2690)
                     /gene="traE"
                     /locus_tag="ACIE68_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFX5910940.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(2712..>3013)
                     /gene="traL"
                     /locus_tag="ACIE68_004787"
     CDS             complement(2712..>3013)
                     /gene="traL"
                     /locus_tag="ACIE68_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFX5910941.1"
                     /translation="ENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITTSKYL
                     FGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWIK"
BASE COUNT          664 a          872 c          754 g          723 t
ORIGIN      
        1 cctccctggc tgccggacat aaactgctgc ggattcaggt tgcccagctg cttcagcatc
       61 tggtcagtat taaagccgtt aagatgactt tcagctgatg gcggaacagg aaccggactt
      121 tccggattat cttcttccgc tctgttctgc gtgcgctcca gcgccatctc ttccacggtt
      181 ttcagctgga agccgtcctg gaacaccacg gtcacttcgt tgcccgcacc aatcggtatc
      241 accgggtgat actgttcggc acgtttgatg tagtagtcac tgagcgtctg tgcggctttc
      301 gatgcgccgc caccgatacc cattttaagg acatcgccgg ccccgtaagc agcagtggca
      361 cccagcccga cagccggctg ggaagcacgc tccattccct gaccgatacc gtcaacaaat
      421 cccgcgcccc atgcccagcc gaggattttg ccgttacgca tcaccacttc gcccttgata
      481 ccgtttttac cccggaagct gacatgcccc ttaatcggca tatcaatagt tttgccgtcc
      541 ttcaggcagc tgatattgcg ggtgcgtaca atggcacgtt cactggacac atccccccag
      601 gcttccagcc ccacaaaaca tcccgttgcg tcatacgttt tgctgttcgg catttccacc
      661 agaccggtga tacgcagctg catcggcacc gtggattcat taccggtgac tgaggcattg
      721 gcatccgccc cttcaatcag catggctttc gcaaaacttc ctgacggaat gtacggcagt
      781 gatggtccct gttttccctc attacgggtg aacaccttac gctgtatccg gttaggcacc
      841 ggtacagact ggtacgtcac ctgtggtggt ggcgtgacac cattcccggg ataaaacgcc
      901 gtcggcggtg gaacagcaac gctgccctgc ggcgggaaag atacgggtgt gtttcctggc
      961 tgtggttcgc cttccgggcc gggcggtgag gctggcgtct gcggtactgg ctcaccggtc
     1021 gcggtgacag gatttgcacc cagggctttc acctgctctg ccagtgcagc attgtcctgt
     1081 cccagctttt caatacgacg ctggtcatca ccgcgctgtt tgttcaggac atccagctca
     1141 cgacggattt cctcatactg cttctgcatc tgcgctgccg tcacctgcat ctctgtggtg
     1201 gcatgctgac gcaccttgtc atcaaaggtc gtatccacca caccggtcat atccggcaca
     1261 ggctcctgtt cagccacggt ttcaccgtta ccggacatgt ccacatcaga cagatacagt
     1321 gccccaccaa tcgcggaggc tgcaccgaca accacaatcc ccagccacag gtactgcttg
     1381 cgtttcacaa tggtattgat actggccatc agttgccctc cccgtcacgg atgacataca
     1441 catccatgcg cgcacctgcc agtaactggc tggcaggctg gctgaacatc acggcacggg
     1501 ttcccggctg ccagaagtca ctttcccgga tattcagggc agacagcgtt ttattttcca
     1561 cggcaaagcg gatcatcttc aggtgattcc ccgtccatac agcatctgcc actgaagaca
     1621 gccccgccgg ggtctgcagg gtttcttttg tcaccgggac ctgataccag cctgcaggta
     1681 attttccgcc acggacggcc tggctgatgg tcacaagcag ggattcgtaa ggcgtggacg
     1741 tttcccacgc accggcttct tctccggttc cgcgcaggtc actgaccagc tgaatggtac
     1801 gccccgcgcc ttcacgggga acggcctgaa tggaaagatt cagaccacgt tctgtttcca
     1861 gaatgaacgt aaagggcttt ttgttgacgg tggccaccac cacaccgccg gaggcggtct
     1921 gctcattatt ggtcagggca ccatccagac tgttcacggc gataatccgg tcaccgggaa
     1981 cggtaaacag attcgggctg gtattgctga tggcaatact ggcctgacca ccgttcacca
     2041 ttggcaccac ggtgggggcc agcgtaccgt ttgcggccat cacgctgcag gaaaaaaaca
     2101 ggctgccgaa tattattgcc gtattatttt ttctcatcgt ctgtttcccc gaaattatcc
     2161 agccaggtca ccccgttttc ccgcttcaga ataaggatgt aatgtttgat atcagtgaaa
     2221 ggttttgaat caccaatcca ggttttaagc acacctcgaa tttccacacg gccatactga
     2281 ggccagacac gaacactggt ctggaaaaag gctgagttca cgttatcgtt tttaatacgc
     2341 ttcgcttctt cagccagaat aactttcatc tggttctgtg cgcccgggcg gatatattgc
     2401 agaagcgcct gatgtgaggc atcgacggtt tctgatgaaa cattcagacg gagggcaata
     2461 aatgacagcg ccatctgctg taaataagag gcgtcggcac tgttctgtga cacggcaaag
     2521 ggggcattaa atgccattgg cgtcacggca gtgcgttgct cattctgaag acggtagtta
     2581 ttcactccct gaatgacgtt aacagagagg ctgagaacaa taagcactga cataaatata
     2641 aaggcgatgg ccattacacg actggtactt aaacgggcac cgtgttccat ataacaatcc
     2701 tggtatcagt tctatttaat ccactgccgg aaacacgaat cgggaacatt atgaaaaata
     2761 ccgcgcaaca gggctgttgg catataccag taaatcaggt cacgtaacca ggaactgccc
     2821 cgcccttttt tcagtttttt aatcccgaaa taaaccagaa ccgctgcacc aataccgaac
     2881 agatatttcg atgttgtgat accccagcca atacagattg ctgcggggat cagttcatcc
     2941 agtggcaggc caaaccagcg gctctggttg gtcagtgttt ccgggaaacg atatttctta
     3001 agtttattct cgt
//
LOCUS       DBANUU010000287         2992 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000287 DBANUU010000000
VERSION     DBANUU010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.293"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            653..1219
                     /locus_tag="ACIE68_004788"
     CDS             653..1219
                     /locus_tag="ACIE68_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFX5910942.1"
                     /translation="MPKSYTPNWFFTALLDNHINQMMARYSCLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTADHGFHAHVVFWIDRQRVKKIY
                     PFVERITECWRSITHNSGSVHRCTYQPHYTYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCNEVPERPAAGRPRKPHF"
     gene            1466..2038
                     /locus_tag="ACIE68_004789"
     CDS             1466..2038
                     /locus_tag="ACIE68_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910943.1"
                     /translation="MNAIPYFDYSLAPFWPSYQNKVIGVLESALREQSGSRIRRILLR
                     LPCEYDRTFSSRKIWFGMDFIETVSALMNATPGRDLCWLLTHHPEKPEYHAVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAKQYQKQITRDVVLDSRSPDCEERLKELIWAF
                     SDFARDRRGVHDPEARYLASNPWYPVAGQL"
     gene            2107..2340
                     /locus_tag="ACIE68_004790"
     CDS             2107..2340
                     /locus_tag="ACIE68_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFX5910944.1"
                     /translation="MLTTTSHDSVWLRADDPLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            2630..2919
                     /locus_tag="ACIE68_004791"
                     /pseudo
     CDS             2630..2919
                     /locus_tag="ACIE68_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          772 a          670 c          690 g          860 t
ORIGIN      
        1 ctgaagtgtt ttaaccattc tggtgagcct caattcttca gtcgtcactg attaactgaa
       61 tgtttattct ttgataaggt atccatgatt ctgtaacgat taacagagct ggataaacaa
      121 cagaatcaac agagtgaatt cctgattatg tttgtttcgg gttgtattgt ttaagatgca
      181 gggtaaaaat catgccagtg gtatttttta ctctggtaat gtgtgcggtt aacgttcatg
      241 tccttaagaa cagattggat gagggcgtat aagtttattg cgttggcctt gtacacgttt
      301 tttcagactc actgagagca agtttgtagt ttatgatgtt attggtcgta atatgtttct
      361 taatctcctg gtagtgggtt tttatcgcgt gttttgcagt atttcgtgat gtttattgat
      421 tctgtatttt attcgctcct cgtttatctc atctctttag ctaataccat cagataatcc
      481 atttctttct gcataatgct gcgtatcgtt aataactcgt cgtatccatt ctgctacagc
      541 atgcctgata aataccatct gtaagttatt accgttttag atctgattat gagcgaaagc
      601 attaattcgc tcacagagct taaaacatca ttaactttca ggagtcatca acatgcctaa
      661 atcttacaca ccaaactggt tttttaccgc tttacttgac aatcacatca atcaaatgat
      721 ggcacgttat tcctgcctgc gggccttacg aatggatttc ttctacagga aagatacgcc
      781 tgatttctta caacctgatc atcgctggct tgaattgcag ttgcgtatgc tgctggaaca
      841 ggttgaacaa tttgaaaata ttgttggctt cttctgggtg attgaatgga cggctgatca
      901 tggttttcat gcgcatgtgg ttttctggat cgatcgtcag agggttaaaa aaatatatcc
      961 ctttgtggag cggattacgg aatgctggcg gtctattacg cataacagcg gttcggtaca
     1021 tcgctgtaca tatcagccgc attatacata caacatcaac attcctgtgc gccacaacga
     1081 tcctgaaagc atcgataata ttcgcggtgc cctgcattat ctggcgaaag aagagcaaaa
     1141 agacgggctg tgtgcctacg gctgcaatga agttcctgaa cgtcctgctg cagggcgtcc
     1201 tcgtaagcct cacttctgaa gcttaaggcc tgagcctccg ctcctggaaa cactccgtcg
     1261 gtaaaaactt accgccttga ttaatgatgt gaaccgaagt caacggagat cattccccct
     1321 gaacctgcat ccagtgttct ttcccttatc ttcccgttct gcttcagttc ttcacttatt
     1381 ccatcaatct catccgcaag ccataacacg tcagctcatt cacgggcagg acgcattgtg
     1441 ggctgcgcat aacggaacat atcttatgaa tgctattcct tatttcgact atagcctggc
     1501 acccttctgg ccatcttatc agaacaaagt catcggcgtc cttgagagtg cgctgcgtga
     1561 gcagtccggc tcccggatac gacggatcct gcttcgtctg ccatgtgaat atgaccgtac
     1621 cttcagcagc agaaagatct ggttcggtat ggactttatc gaaaccgtca gtgcgctgat
     1681 gaatgcaaca cctggacgag atctctgctg gctcctgacc catcatcctg aaaaacctga
     1741 ataccacgcg gtgctgtgcg tcagacagga gtatttcgat ggccctgaac tggaccggtt
     1801 gatactggat gcctggagta acgtactggg cttcgcgtca ccgggtgaag caaagcagta
     1861 tcagaagcag atcacccggg atgtggtgct ggacagtcgt tcaccggact gcgaagagag
     1921 actcaaagag ttgatatggg cgttcagtga tttcgcccgc gatcgtcgtg gtgtgcacga
     1981 tccggaagcc cgttacctcg ccagtaatcc ctggtatccc gtagcaggcc agctctgaaa
     2041 gctcggttca gcatctttcc ctgcattcgc ctaaccttca taaatctcac tgaggacatt
     2101 ctgaccatgt tgaccacaac aagccacgac agcgtatggc tgcgtgccga cgatcccctg
     2161 atcgacatga actacatcac cagtttcact ggcatgaccg ataaatggtt ttacaagctg
     2221 atcagtgaag gccatttccc taaacccatc aaactggggc gcagcagccg ctggtacaaa
     2281 agcgaagtgg agcagtggat gcaacagcga atcgaggagt cacgaggagc agcagcatga
     2341 aacgtgttgt aatgccggta cgctggcaat gtgcaaaatg ccagcgctgg tattgtggaa
     2401 cccggccctg cccctggtgc tggcgacatt tacgttcatc ttcttgcaga cattctccag
     2461 tcagcctgca ggttatagct attcctgact gattcgtcat tttattctta ttgattataa
     2521 ctggcattac accggtgccg gagtgttttt ctgtaagtct gcgccggttc agtaaattag
     2581 aaaagaaaca tttgtcagcg ccagtgatat aaggcgtaac ggctaacaat ccagagcaag
     2641 tcatattcat gatgtgacaa tgaaaatgcc aatggggtat gaaacgttaa taggtgaact
     2701 gggggaaggt ctttccggcg gtcaaaaaca gcgtatattc attgcccgag ctttataccg
     2761 gaaacctgga atattattta tggatgaggc tacaagttct cttgatacag aaagtgaacg
     2821 tttcgtgaat gctgccataa aaaaatgaat atcactcggg tgattattgc acacagagaa
     2881 actacgttga gaactgttga caggattatt tctatttaaa atccactggt gtaactttgt
     2941 aaggagtttt gtctatgggg gaggttaaga aggatataaa aataacagtg at
//
LOCUS       DBANUU010000288         2965 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000288 DBANUU010000000
VERSION     DBANUU010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.294"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..566)
                     /locus_tag="ACIE68_004792"
     CDS             complement(<1..566)
                     /locus_tag="ACIE68_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HFX5910945.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIV"
     gene            759..1262
                     /locus_tag="ACIE68_004793"
     CDS             759..1262
                     /locus_tag="ACIE68_004793"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFX5910946.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(1308..1724)
                     /gene="rraB"
                     /locus_tag="ACIE68_004794"
     CDS             complement(1308..1724)
                     /gene="rraB"
                     /locus_tag="ACIE68_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFX5910947.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1886..2899
                     /gene="argF"
                     /locus_tag="ACIE68_004795"
     CDS             1886..2899
                     /gene="argF"
                     /locus_tag="ACIE68_004795"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFX5910948.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT          859 a          691 c          666 g          749 t
ORIGIN      
        1 acaataccaa ctattgccat cagaaccaca gatataataa cactacttga actattggca
       61 tgtagcatct ttgtcgagat aaagaaaaca gtccccatcc caatggccat taaaatcggc
      121 aagaaaaagg ttacccacca gaaccctttc aaagagcaat taaaactaaa tcttacgccg
      181 ttgagactag tcatcaaggc ctgataacgc agtccttttg ctgccataaa tataagcaaa
      241 accaacagtg acaaagtcaa aacacagcca acaagcggca tatctgctga cactgtcatt
      301 aagattgcga aatagaaaaa aacaaaaaca agacaactaa caaaaacatt cccaccggta
      361 atcccataag aaaatcgttg tccgttaact tccatattag catagagata acgcttacat
      421 ttcattaatg cccatggtaa atagatacct agcgttataa tcgttaacaa cacattcacc
      481 acacaaataa gaaaatatct ctcacctgta ccatgaaaaa caaacgaatg ggacggaaca
      541 tccatttcat taataacttg agccataaaa attccctgaa ttaaaaaatc cattgatata
      601 atttatagat acagacagta aaatgttctg ccacgttaca atactcttat cagtaatagg
      661 ctggcaaaaa ctttattgct ttataagacg cttgcgcacg taagaagaga gtggtattaa
      721 acaaatgtat aattacttta tttttattgg gttaaactat gaataacatt gcgccgcaat
      781 cacctgtaat gcgtcgcctg acgttgcagg ataatcctgc tatcgcccgc gtcattcgtc
      841 aggtatccgc cgaatacggt cttaccgctg ataaaggcta caccgtcgcc gatccgaatc
      901 ttgacgaact gtatcaagtg tatagtcagc ctggtcatgc atattgggtg gttgagtacg
      961 agggtgaagt ggtcggcggc ggtgggattg cgccattagc aggtagtgaa tcggacattt
     1021 gcgaactgca aaagatgtat tttcttccgg ctattcgcgg taaagggctg gcgaaaaaac
     1081 tggccttaat ggcgatggag caagcacgag agatgggctt caaacgctgc tatctggaaa
     1141 cgaccgcttt tttaaaggaa gccattgcgc tttatgagca tttgggcttt gagcatatcg
     1201 actatgcgct tggctgcacg ggccatgtcg attgtgaagt gcggatgcta cgcgaactct
     1261 aaaaaaatgc ccgctagtgt gtgctatcgg gcattttaat caattaatta gtggcgaacc
     1321 ccgtcatcgt cttcatcgac aaaatcttca tcgtcgccgt cttcgccgtt gggatcttca
     1381 aagtaagtgc cccaaccgtc gtactcaacg tcaaatttct ccgccagcgt catcagttgt
     1441 tcaacctggg catcgatcag atcggcattc aacgcgcact cgctgaggat gtcgcagcaa
     1501 atcacgatat caccatcttc aacttccagc tcttctggat cggtcacttc gtaaccgagt
     1561 ttaaacgctt caactgctgc tttttccagg gtttccagat cgtctgcgga aagatggtgt
     1621 tcgatggtgt acagcgcgtc cggatcgctg ccatcttcca gtaattcttc aataatcaaa
     1681 cgtgtttctt cacgctgttc ttccagttgt tccgggtttg ccatggctca ttcctcttaa
     1741 tgtcctgccg atactcttat tgtcacacac cactgacatt gcctccacct ttgtgacaaa
     1801 gatttatgct ttagacttgc aaatgaataa tcatccatat aaattgaatt ttaattcatt
     1861 aaggtgttag ccacaggagg gatctatgtc cgggttttat cataagcatt tcctgaaatt
     1921 actcgatttc acgccagctg aactcaacag cctgctgcag ttagccgcga agctgaaagc
     1981 cgataagaaa agcggcaaag aagaagccaa actcactggt aaaaacatcg cgctcatctt
     2041 cgaaaaagac tcgacccgta cccgatgctc tttcgaagtt gccgcatatg accagggtgc
     2101 tcgcgttact tatctcggcc caagcggcag tcagattggt cataaagagt cgattaaaga
     2161 cactgcccgc gtgctgggtc gcatgtatga cggtattcag tatcgcggct atggtcagga
     2221 gattgtcgaa acactggcgg aatacgctgg cgtgccggta tggaacggcc tgaccaatga
     2281 gtttcacccc acgcagttgc tggcggatct cctcaccatg caggagcatt tacccggtaa
     2341 agcattcaac gaaatgacgc tggtctatgc aggtgacgca cgtaacaaca tgggcaattc
     2401 gatgctcgaa gctgcggcgc ttaccggtct ggatttgcgt ctggtcgcgc cacaggcatg
     2461 ctggccggaa gctgcgctgg ttacggaatg ccgcgctctg gcacagcaaa atggcgggaa
     2521 tattacgctg acggaagatg tagctaaagg agttgaaggt gctgacttta tctataccga
     2581 tgtatgggtg tcgatgggtg aagcaaaaga gaaatgggca gaacggattg cattactgcg
     2641 tgattatcag gtgaacagca agatgatgca gttgaccggt aacccggagg tcaaattcct
     2701 ccactgcctg cccgcgtttc atgacgacca aacgacgctt ggcaagaaaa tggcggaaga
     2761 gtttggccta catggcggaa tggaagtgac tgatgaggtc ttcgaatctg ccgccagcat
     2821 tgtatttgat caggcggaaa accgcatgca taccatcaaa gcggtgatgg tcgcgacgct
     2881 cagtaaatta aataattaat atctctatca agatgccggg aaatacaaaa acatcccggc
     2941 atcacattat tttatgcagt gatag
//
LOCUS       DBANUU010000289         2918 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000289 DBANUU010000000
VERSION     DBANUU010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.295"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..132
                     /locus_tag="ACIE68_004796"
     CDS             <1..132
                     /locus_tag="ACIE68_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFX5910949.1"
                     /translation="SRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
     gene            411..561
                     /locus_tag="ACIE68_004797"
                     /pseudo
     CDS             411..561
                     /locus_tag="ACIE68_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(779..2869)
                     /gene="iha"
                     /locus_tag="ACIE68_004798"
     CDS             complement(779..2869)
                     /gene="iha"
                     /locus_tag="ACIE68_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFX5910950.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLFSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
BASE COUNT          706 a          799 c          671 g          742 t
ORIGIN      
        1 agccgggatg aggccatctc agtaatacgg gaatacattg agattttcta caatcgtcag
       61 cgtcgtcact ctcgtctggg gaatatctcc ccggcagcct tcagggaaaa atatcatcag
      121 atggctgctt aaaaaaagaa caaatggtag tgtccgctat tgccagtaca cctcagtgac
      181 ctgctttatc aaacttaatt gtgacaaaat ggcgattgag aaggtgcaat tgacttatcc
      241 ggattcggtc gtggcgaggt ggtgaccctg cattctgcta accgggttat gagccaggag
      301 tcagcgatag acaaagggac ttatctgacc gggcttccgg aaaaatggcg ggagagaatg
      361 ataccttcct gctgacgaga ccggactttg agatgacgag cgatagtgcg gcagtgatgt
      421 acagcctgat cggtagctgc aaaacttaac ggaatcgagc cggaaacgtg gctacgccac
      481 gtgatcagtg taatcaacac ctggcctgcc aaccgcatga aagagttgtt gccctggaac
      541 gtcactctct ctgtcaacta atttttaccc ctcgtccttc acggtgcgct tacactctgg
      601 acaagtatgt aaatgataag ttaaaaataa tccgtgcatt tcctgtgacg atattagttg
      661 ttggtaacga aatctcatta gcggatcgga aatgcactta acctaatacg ttgccactgt
      721 tccgccagga gttgcagtgg caacgtattc taccgtcagt gatagcgttt tgttattatc
      781 agaactgata gttcagcgac atccagtaat ttcgctcagg tatcacatat cctgttgttg
      841 atgatcccgt ctggaagtaa tcaccggcat acagcgtact cttaccggca ctgaacaggc
      901 tcacgtcact gtaatccttg ttgagcaggt tattcaccgc agcattcagc gtcagggcat
      961 ccgtcatctt ccacgacaga cctgcatcca ccaccgtcca ggctttcagg tattctcctt
     1021 tctcatcata cactttcttc tgtacagcgc tcagtgacga ataattctgg gtgaaacgtg
     1081 gtgttttccc gcgataacgg gcacccagcc atgatgccac ctcttcggtg atctgccagt
     1141 tcagtttcgc attcaccatg tgttcagggg tataactcag cggcgcacct ttgttatcac
     1201 catcacgttg ttcacttcgg gtccaggtgt aattcagtga cagcgtgaca tcctctgacc
     1261 acagcggcaa tgtgccggca aattccacac cgtgcaaccg ggcctttccg ctgtttacat
     1321 agctattggt gttatcattt atggaataag agacaatctt gttggagaag tcagtcataa
     1381 aacctgtgac attggcattc agaccggcgg ggttatcgta atacacccca gcctcataac
     1441 tgacgctctc ttccggcttc aggtcggggt taccaagtag atttgttttt ccctgcccgg
     1501 acacaccact aatcccttta tgtagctgcc ccattctggg tgccttatat cccgtggtca
     1561 caccgccttt cagcgtccag gcatctgcca catcccagac cagatatgca cgcggactga
     1621 agtgtccccc gaattgctca tgatgttcat agcggctgcc cgcagtcagc gcaagtgcat
     1681 ccgtgagatg ccactcatcc tcagcaaata ccgaccagct tttctgccgg aaagtttcac
     1741 ctgtgctggc aaggacaact ccgtctttca tggacgagct ctgaaactcg ccccccaccg
     1801 taaccagatg agattctccc agaggggtaa gcagtaatga attcaggata aggttcgatt
     1861 ccttaagctc ccgcggctga ccggcaagcc cccatttgtc gcgcttcagt acactgcgta
     1921 cgagctcacg acctttattt tctgtctcgt tccagttcag atacgatttc catgttccga
     1981 aggtgaaagt atgatcatag ccagctgaaa ttttgtttcg ctcatagcgc agggtccggt
     2041 catatccccc cgtcagactc cccagttgcc catcccggtt atcataacgc tgccgggtgg
     2101 tatccatatc aaaccagagc acatcctgct ccgacgcctt ccagtcaaga cgtgcaccaa
     2161 gattataatt ctgtgactcc gtgggataag gaatacgcgt ggctgctgta tcgctcagtg
     2221 atgtgaccga tgagccctga cgctgttgtg tgctaccgcg tacctgcagg ctgacagaat
     2281 catccacaag gggaccactg ctccagaaat taaactggct gctgttaccc catttgttgc
     2341 tttcctgcag attcagccct gcattgacgg aagagagcca tttgtctgca ttctttctgg
     2401 taatgatatt caccacaccg cccatcgcat cagagccata cagtgtggac atcggccccc
     2461 tgataacctc aatacgctca atggcggcca gagggggcat gaacccggta ttcatggcag
     2521 aaaaaccgtt gggagtcacg tcactgcttc cgccctgacg aacaccatca atcagtatca
     2581 gcgtgtaact ggctggcatt cctcggatgc tgatttccag cccaccggtt ttacccgtac
     2641 cactttcaac atccacaccc tctactgatc tcagagcctc cgccagatcg tggtactggc
     2701 tggactgcaa ttcctcctgg ctaatcacag aaacactggc ggctgcgtta gtcagctttt
     2761 tctcatagcc ggatgccgag acaatcatca catcctctgc agcagctatg ctgctggctg
     2821 aaaatccgag acagggaatg actacggaag ccagagtggt tattcgcata ttactaatct
     2881 ccgatcatgt taaccggcaa gacaatacac aaatacaa
//
LOCUS       DBANUU010000290         2918 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000290 DBANUU010000000
VERSION     DBANUU010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.296"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            237..581
                     /locus_tag="ACIE68_004799"
     CDS             237..581
                     /locus_tag="ACIE68_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004026082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFX5910951.1"
                     /translation="MPYSAKRYQTENGEVPYTDWMKKLRRKDQTAALKVDSRIARAMS
                     GNFGDHKFERDGVWELRVDYGPGYRVYYSIEDGEIILLLIGGNKKTQTVDLDKAVSYL
                     QDFKKRSKNDSH"
     gene            568..918
                     /locus_tag="ACIE68_004800"
     CDS             568..918
                     /locus_tag="ACIE68_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFX5910952.1"
                     /translation="MTATKNVKSQKNDYPASVDHNAAMIEELRADPDYANAYLANALE
                     EINEPGGLGGFLVALRQVIEARGGISEAARKSGLARQSIYRALSPNGNPTITTLAQLT
                     SVAGLQFTLSKSSH"
     gene            1752..2267
                     /locus_tag="ACIE68_004801"
     CDS             1752..2267
                     /locus_tag="ACIE68_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFX5910953.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELM
                     KAVNAAFDVLMANIDKINQFQSADEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVW
                     ISGETITHKETLKEIGCKWAAKKKKWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRR
                     ATGWQRVETRA"
     gene            2371..2913
                     /locus_tag="ACIE68_004802"
     CDS             2371..2913
                     /locus_tag="ACIE68_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910954.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCPSRIKLTSPQFKN
                     YSVHIRMEKDRFSVVGSVDSRSWSSPYHVCTLSRKRNPVDIAANIERKILLNASQEVL
                     QAIEYEKRQAAKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGERYDL
                     QIRGLSIDQLVKITGYLKQL"
BASE COUNT          854 a          581 c          729 g          754 t
ORIGIN      
        1 gaacggagta cgtggtaaca ggggcttaat tgtccctttt tatccggtgc gctatttgat
       61 aaaacattaa gtgtgtatta atacacattt ttagggcgtg atttctggtt gtttttgtaa
      121 aagccaagtt ctatcacgcc agactcacat tttgtcactt taaagagaca ttcttcttga
      181 tctgatgtca cttataagag acaatgtgca caggttaagg gaatgggggc ggcaagatgc
      241 cttactcggc gaaacgatat cagacagaaa acggtgaggt tccatacacc gactggatga
      301 aaaaattaag acgtaaagac cagaccgcag cgttaaaggt ggattccaga attgcccgcg
      361 ctatgagcgg aaattttgga gatcataaat tcgaaaggga cggtgtctgg gaattacgag
      421 ttgattacgg tcctggttac agggtttatt attcaatcga agatggcgaa ataattcttc
      481 tgctcattgg agggaataaa aaaactcaga cagtcgattt agataaagcc gttagttatc
      541 tgcaagattt taaaaagagg tcaaaaaatg acagccacta aaaatgtaaa gtcgcagaaa
      601 aatgattatc cggcatccgt tgatcacaat gctgccatga ttgaagaatt gcgcgcagat
      661 cctgattatg caaacgctta tcttgctaat gccctggaag aaataaacga gcctggtggt
      721 ttaggcggtt ttcttgttgc gctacgtcag gtaatcgagg cgcgaggggg gatttccgaa
      781 gccgccagga aatcggggct tgcccgtcag agtatttacc gcgcattatc cccgaacggg
      841 aacccgacca tcacaacact tgcacaactt acatctgtag ccggattaca gttcactctc
      901 agtaaatcca gccactaacc tctccagttc cccctaagcg ggggaatttc ctcatgtcag
      961 caaaaaatca tcaaacccgc agttgtcccg accgaagttc actggtattt atcgcgggga
     1021 ataatgccac cgtacgagtg gaaacatgtg tatttctgtg tgcccctgta taatccggtg
     1081 atttgtctgt gactggaacc ttaaagctaa tgtatgcata cattagcttt aagcctgtat
     1141 tcccatcgtt atcgaggccg cctggaaaac ggaataaacc acgttaagag tattttttag
     1201 ccacatggct tttgggaatc gccagaatgg cgggataact gtggttttgg tgtctgaaat
     1261 cgtacaattg tatactggca atagtttatt ggtgtgtatt aatacatacc ttaagtcgat
     1321 gtgatttctg gcgcgttgcc cccgtagctc gctggcgctc acacgcgcag tacagcgccc
     1381 cgtccaggga aaccctggac ggcgtaaact cgctggcgcg agcgctaact gcggcattct
     1441 gcggcgcaaa tcagcgttat cgttgtctgt gagtcagtcg ggcttagcag gggtggaagg
     1501 tggccctggt tccggttgat aacagtagcg gcgtagccgc gcttcggggc gatgtggctc
     1561 ctgtgctacc ctggaatttt cgttcgcccc ttacatccgc tggcgcggat aaaaagtgtg
     1621 acaatcatca aataatttac cttttttcag ggcagaagcc ttgccaatac atctaaatag
     1681 atgtattatt gtttcatcga aaggagatag gcttctttcc ttcttaagtc caaacggaca
     1741 ggagatttaa aatgaatatc caggaagcat taaacgtttt tggattatcc ggcgaattaa
     1801 ctgaaaaaga tatcaaagca gcatacagaa aagccgcttt aaaatatcat ccagatcgta
     1861 acccgttagg ggctgaactg atgaaagcgg taaatgcagc ttttgatgtc ttgatggcaa
     1921 atattgataa aataaatcag ttccagagcg ctgatgaaca tgcacgatat aattacggtg
     1981 atgacctgga aaaagtatta aacgttcttt ctggtttatc tggtctggta tttgaagtga
     2041 taggtaactg ggtatggatt agtggagaaa ctattacaca taaggaaact ttaaaagaaa
     2101 tcgggtgtaa atgggcggca aagaaaaaaa aatggtttta tcgtccggac gaacataaaa
     2161 gttactggaa tcgtgaagaa cacacgatag aagaaatccg cgcaaaatac ggtacaaccg
     2221 gacagcgcag ggcgacaggg tggcaacgcg tggaaaccag agcgtaacca gaacgggggc
     2281 gagaagcccc cgatatcata ccagcccgtc ttttggggca tcgaacccaa aaagacggga
     2341 acagcagcca cgtagcaaaa agggaaacag atgaactctt tttttgaaca atatcaccct
     2401 gtctttgaag ttgtctgccg tatcctgggg aacggctggc gcgtgaacaa acttgatgat
     2461 tgtccgtctc gtataaaact gacgtcaccg cagtttaaaa attactctgt gcatattcgg
     2521 atggaaaaag accgattttc tgttgtgggg agtgtggata gtcgttcctg gagtagtccg
     2581 tatcatgttt gcacgttatc caggaagcgg aatcccgttg atatagcagc caatatcgag
     2641 cgaaaaattt tgctaaatgc ttcgcaggag gtgttacagg cgattgagta tgagaaacgc
     2701 caggcggcaa aaaaggacga aattctgatc ctgaaaggta tgttatcgca acttgtccag
     2761 cttgaaagct ggtatggggc attgacaggc tttaaagctg aaaatggatt gaacggtaaa
     2821 gtcaccgagc agggcgagcg ttatgatttg cagattaggg gtttgagtat agatcaactt
     2881 gttaaaatta caggatattt gaaacagtta tgagaaaa
//
LOCUS       DBANUU010000291         2895 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000291 DBANUU010000000
VERSION     DBANUU010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.297"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            110..2107
                     /locus_tag="ACIE68_004803"
     CDS             110..2107
                     /locus_tag="ACIE68_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFX5910955.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVSLALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2170..2508
                     /locus_tag="ACIE68_004804"
                     /pseudo
     CDS             2170..2508
                     /locus_tag="ACIE68_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
     gene            complement(2518..>2895)
                     /locus_tag="ACIE68_004805"
     CDS             complement(2518..>2895)
                     /locus_tag="ACIE68_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFX5910956.1"
                     /translation="TMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQ
                     SMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPHEYN
                     GGLPPNESENRYWKNSNAVASFC"
BASE COUNT          642 a          705 c          731 g          817 t
ORIGIN      
        1 cattccacca tgcattccga ttaacgccgc atagccagtt gaactttgct actttgtgag
       61 aggtagtacc ttctatccag tgcgaattta attaatggaa taaatgatta tgagtgaaaa
      121 tgatacaatc ccaaagaagt ctacaagtca gattaacaaa gcggtattct ttacatctgc
      181 tttgctaatt ttccttcttg tcgcctttgc cgccgtattc ccggatgtcg ccgacaaaaa
      241 ttttaaacta cttcagcaac aaatcttcac gaatgccagc tggttctaca tccttgctgt
      301 ggccctgatt ttactgagtg tcacgttcct tggactctca cgctacggtg atatcaagct
      361 gggcccggac catgcgcagc ctgatttcag ctaccactcc tggtttgcga tgcttttttc
      421 ggcagggatg gggatcggcc tgatgttctt tggcgttgcc gaacctgtaa tgcattatct
      481 ttcgccaccc gttggcactc cagaaaccgt tgcggcagcc aaggaagcaa tgcgtctgac
      541 ctttttccac tggggactgc acgcatgggc aatttatgcc attgtggcgc tgattctggc
      601 gttcttcagt taccgtcacg gtctgccttt aactctgcgc tccgcactct atcccattat
      661 tggcgatcgc atatacggac ctgtaggaca tgcggttgat attttcgctg ttataggcac
      721 ggtctttggc gttgcgacat cactgggtta cggtgttttg caggtgaatg ccggtttgaa
      781 ccatcttttc ggggtgccca tcaatgaaac ggtgcaggta attctgatcg tggtcatcac
      841 ggggttagcg acgatttcag tggtgtccgg tctggataag ggaatacgta tcctgtctga
      901 actcaatctg ggtctggctt tgttgctcct ggcgctggtc ctgtgtctgg gaccaaccgt
      961 gcttctgctg aagtcatttg tggaaaatac gggcggttat ctttcggaac tggtgagtaa
     1021 aacgttcaac ctttacgcgt atgagcccaa gtcgagcaac tggctggggg gctggacatt
     1081 actgtactgg ggatggtggc tttcatggtc gccgtttgtg gggatgttca tcgcacgggt
     1141 ctcccgcggg cgaaccattc gcgagtttgt caccggcgtg ctgtttgttc ccgcgggttt
     1201 tacgctaatg tggatgacgg tgtttggtaa cagcgcgatc tatctcatta tgaaccaggg
     1261 ggccacagac ctcgccaata ctgttcagca ggatgtgtcg ctggccctgt ttaatttcct
     1321 ggagcatttc ccgttctctt ctgtgctgtc attcattgca atggcgatgg tcatcgtctt
     1381 ctttgtaacg tctgctgatt cgggggcaat ggttgtggat actctggcat caggtggagt
     1441 ggcaaacaca cccgtctggc agcgaatatt ctgggcctcg ctcatgggca ttgttgcaat
     1501 tgcgcttctc cttgccggag ggctaagtgc gctgcaaacg gtgacaatag cgagtgcatt
     1561 gcccttctca gtgatcttac taatatccat atacggactt ttaaaagctt tgcgccggga
     1621 tttgaccaag cgtgaaagcc tgagcatggc gacaattgct cctacggctg cacgtaaccc
     1681 aattccttgg cagagaaggt tacgcaatat cgcgtatctg ccgaagcgat ctcttgtgaa
     1741 acgttttatg gacgacgtta tccagcccgc catgacgctg gttcaggagg aactgaacaa
     1801 gcaggggacg ataagccaca ttagtgatgc agtcgacgat cgtattcgtc ttgaagtcga
     1861 tttgggcaac gagctgaatt ttatatatga agtgaggctt cgcgggtata tctcaccgac
     1921 cttcgcgctc gccgcaatgg ataatgatga gcagcagact gaacaacatc gatattatcg
     1981 cgctgaggtt tatctcaaag aaggcggtca aaattatgat gtgatgggct ggaaccagga
     2041 acagctgatt aatgacatac tggaccagta cgaaaaacac ctgcacttcc tgcacctggt
     2101 tcgttaatag caacatgccg tcctgggggc ggcaattatt atctcggccg caatatgagg
     2161 gaatgcagaa tgatttcacg ctggaaatgg atgctgaagc agacaattaa aaaactatgg
     2221 ttcagggcaa cgttattcgc aattgtcgcg ataataacgg cccttttatc aattcttttt
     2281 aaatcaatga tacctgagtc ggtttccgtg aaggttggtg cggaagcagt cgataacatt
     2341 ctgaacatac tggcatcgag tatgctggca gtgaccacat tttcgctgag tatcatggtc
     2401 acagcctacg gttcagccac tactaatgtg actcccagag ctacgcgttt agttgttgaa
     2461 gacgtcacca cacaaaatgt actggccacc ttcatcggtt cttttctctt gaagtggtca
     2521 acaaaaactg gccaccgcgt tagagttttt ccagtatcga ttttccgatt cgtttggggg
     2581 taacccaccg ttatattcgt gcggtcttag tgcgctgtaa tatccaacga tatagtccgt
     2641 tatggcgtga gctgcctcgc tgaagcttac gtaacccacc accggcatcc attcgttctt
     2701 cagactcctg aagaagcgtt ccattgggct gttatcccag cagtttccgc gccggctcat
     2761 actctgtctg atctggtatc gccacaataa ctgccggaac tgcctgctcg tataatgact
     2821 gccctgatcg ctgtggaaca tcaccccgcc gggcttacca cgggtttccc atgccatttc
     2881 cagcgctttc atggt
//
LOCUS       DBANUU010000292         2882 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000292 DBANUU010000000
VERSION     DBANUU010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.298"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..812)
                     /locus_tag="ACIE68_004806"
     CDS             complement(<1..812)
                     /locus_tag="ACIE68_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjP family GTPase"
                     /protein_id="HFX5910957.1"
                     /translation="MSFSHSSLSAQVKSHLTFLPEEIRQKILEHLHSVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHRLTLQFGERRMTLVDLPGIGETPQH
                     DQEYRTLYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNATEKCPSRQQSLSLATVTARVATLFPSSFPVLSVAAPVGWNLPAFVSLMI
                     HALPPQATSAVYSHIRGENRSEQAQKHAQQTFGETIGKSFDVAVARFSFPAW"
     gene            918..1880
                     /locus_tag="ACIE68_004807"
     CDS             918..1880
                     /locus_tag="ACIE68_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910958.1"
                     /translation="MTAQQSDALREIANKARVTTILQCKAWKDTQRILKRSGLVCRER
                     SEPFDPEKHFDCYTVRYLYLLNIMALELKSDTRIKVEVGQWYRMTGKRLSLNVPPFML
                     IPRNIRRKVDGFRQSRQSEDEATKNPPQPFTGSLYKVLSRDSDSAELDAWFAEPPLTR
                     QEVWEGRRVTDFDPWALSSFICRSESPTFELFYQEYKRLGLKSLFVSGVMFEQFLTGL
                     SFRKYGDWVESQLLESLGNVMFFMLLYDMENLDKFIKELMDINVQSEDSKEKGKSRKE
                     RMLEYINSYIRNVYGRFLCTSKERYEQHKRKNSSKKKNGSGGTH"
     gene            complement(1877..2731)
                     /locus_tag="ACIE68_004808"
     CDS             complement(1877..2731)
                     /locus_tag="ACIE68_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory RepB family protein"
                     /protein_id="HFX5910959.1"
                     /translation="MSVHSPDESDHVFSRMEYEYYRHNDKSPSVNADNAGRDVVPEWV
                     QKDPVAAFWVWMFIYHVPDSIVRLSGEQYADYGTSCLYARLFATEAAPANHQARIRVT
                     RKWLQELIRLTGTENVSDFLYVVHFLWSYAEKVNPMTWLGSRRIHIKWAWAYLQKHCT
                     DEERHCPDISEFKASGESRLFIIAYVRRVFMKIYSVGTEPQKLKELVYQQNDFLRRMK
                     CSYQKMFRYEKTDSRVQISALISPEARNALRKMAKKNNLTQAEMLDKLILSADRLFMQ
                     KTAGDYSR"
BASE COUNT          808 a          630 c          696 g          748 t
ORIGIN      
        1 atccaggccg gaaaactgaa acgggcaacg gcgacgtcaa aacttttccc gatggtttca
       61 ccaaaagtct gttgtgcgtg tttctgggcc tgttcagagc ggttttctcc ccggatatgt
      121 gaatacaccg cgctggttgc ctgtggtggc agtgcgtgga tcatcagcga cacaaacgcc
      181 ggaaggttcc agccgacagg tgcagcaacg gagagtaccg gaaatgatga agggaacagg
      241 gttgccaccc gggctgtcac cgtcgccagt gagagcgact gctgacggga cgggcatttt
      301 tctgtggcat tccattcttc agcaggaaac acgcgatcgg catggctgag aacaaacaga
      361 aaacgcgagg gatctgcgcc ttcattcagt aaaaactgat gcatggcaat atcagcagca
      421 tacgcacgtt catcagcccg caggatccag ataatcagat ccagttccgg tagcagctga
      481 cgataaagtg tccggtattc ctgatcatgc tgcggtgttt caccaatgcc gggcagatcg
      541 accagcgtca ttctgcgttc accgaactgc agggtaagac gatgagcctg gcgggtgcag
      601 ccgttcagag gatgtgtggc gcagatacgg gactgaaaaa tggcattaca cagactgctt
      661 ttgcctgttc cggatttacc cataatgcca atcacgggct cgtaatgaat aacactgtgg
      721 agatgttcaa ggattttctg gcgaatttct tcaggaagaa acgtcagatg tgatttcact
      781 tgtgcggaga gggaagaatg gctgaatgac ataccgttat cctgttgatt tcgctgaacc
      841 ggatcacact aatccggctt atcattcagg taatatggac gtgaaaaaat ctggagtgac
      901 agagtgcggg aggtgagatg acggcacaac aaagtgatgc gctgcgtgag attgctaata
      961 aggccagagt taccaccatt cttcagtgta aggcctggaa agatacccag cgaatactga
     1021 agcgcagcgg actcgtgtgc agagaacggt cagagccgtt tgatccggag aaacattttg
     1081 actgttatac ggtgcgttat ctgtacctgc ttaacataat ggcacttgag ctaaagtcag
     1141 atacacggat taaggttgag gtggggcaat ggtatcgcat gacgggtaaa cgtttatcac
     1201 ttaatgtccc gccttttatg ctgatcccgc gtaatatccg gagaaaagtg gacggattca
     1261 gacaatccag acaatcagaa gatgaagcaa ccaaaaatcc gcctcaaccg ttcacgggca
     1321 gtttgtataa ggttttgagc agggacagcg attcggctga gctggacgcc tggtttgctg
     1381 aaccgccgct gacacggcag gaagtgtggg aggggaggcg tgttacagat tttgatccat
     1441 gggcgctgtc ttcgtttatt tgccgttcag aatccccgac gtttgaactt ttttaccagg
     1501 aatataagcg actgggatta aaatctcttt ttgtatcagg ggtaatgttt gaacagtttt
     1561 taacagggct aagtttcaga aaatatgggg actgggttga atctcagtta ctggaatcac
     1621 tgggaaatgt gatgtttttc atgctgcttt atgacatgga aaatctggat aagtttataa
     1681 aagaactgat ggacattaat gttcaaagtg aagattcgaa ggaaaaaggg aaatcacgaa
     1741 aagagagaat gctggagtat atcaactcat atatccgcaa tgtttatgga agattccttt
     1801 gtacgtcaaa agaaaggtac gaacagcata aacgcaaaaa cagcagcaaa aagaaaaacg
     1861 gttctggtgg aactcattaa cgggaataat cccctgctgt tttctgcata aacagacgat
     1921 cagctgataa gatgagttta tccagcattt cagcctgcgt cagattattc ttttttgcca
     1981 ttttccggag tgcattcctg gcttccggag aaatcagggc acttatctgt acccgggaat
     2041 cggttttctc ataccggaac attttctggt atgagcactt catgcgcctg agaaaatcat
     2101 tctgctgata aactaattct tttaactttt gaggttcagt accgacagaa tagattttca
     2161 tgaagacacg gcgcacataa gctattatga acagacgtga ctcccctgaa gctttaaatt
     2221 ctgaaatatc aggacagtga cgttcttcgt cagtgcaatg tttttgcaga tacgcccatg
     2281 cccatttgat atgtatccgc cgggaaccaa gccatgtcat ggggttcact ttttcagcat
     2341 atgaccataa aaaatgaacg acatacaaga agtcagatac attttctgtc ccggttagcc
     2401 ttataagctc ctgtagccat tttctcgtca cacgaatcct tgcctgatga ttagctggag
     2461 ctgcttctgt ggcaaacagt ctggcataca gacaggaagt tccgtaatct gcatattgct
     2521 cacctgagag tcggacaata ctgtcaggaa catgatatat aaacatccat acccagaatg
     2581 cggctacagg gtctttttgc acccactccg gaacaacgtc tctgcctgca ttatcagcat
     2641 taacagaagg tgatttatcg ttatgtcggt aatattcata ttccatccga ctaaaaacat
     2701 gatcagattc atctggtgaa tgcacagaca tgaactccct gggcataaat gcacaaccaa
     2761 ctctatagaa ctctttaccc cccctcttat cacctctgtc agtaaatttc gcctgaatcc
     2821 ccttttatcc atacatcaga aaaaatgcaa taacgttcat ggactctttt ctctgattta
     2881 ca
//
LOCUS       DBANUU010000293         2861 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000293 DBANUU010000000
VERSION     DBANUU010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.299"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            150..2429
                     /gene="ydeP"
                     /locus_tag="ACIE68_004809"
     CDS             150..2429
                     /gene="ydeP"
                     /locus_tag="ACIE68_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFX5910960.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKNGAQAVTVEDSMSMIHASRGMLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2677..>2861
                     /gene="safA"
                     /locus_tag="ACIE68_004810"
     CDS             2677..>2861
                     /gene="safA"
                     /locus_tag="ACIE68_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFX5910961.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIIN"
BASE COUNT          770 a          659 c          688 g          744 t
ORIGIN      
        1 taatctattg tttaacgata atagattaaa aatagaaaga gagtagtccc ctactcttat
       61 atatccatgt tggcgataat ccctttgtat atactgtgca tcatcgctat tacaaatcct
      121 aataattcat ttccacacag gataagtaga tgaagaaaaa aattgaatcc taccagggtg
      181 ctgccggtgg ttggggtgct gttaaatccg tagcgaatgc agtacgtaag cagatggata
      241 tacgccagga tgttattgcc atgtttgaca tgaataagcc agagggcttt gactgtccgg
      301 gttgtgcatg gccagatcct aagcacagtg cgtcattcga catttgtgaa aacggcgcaa
      361 aagcaatcgc ctgggaagtc acggataagc aggtaaatgc ctctttcttt gctgagaata
      421 cggttcaatc attacttacc tggggagatc acgagcttga ggctgcggga cgtctcactc
      481 agcctttgaa atatgatgcc gtcagcgact gttacaagcc attaagctgg caacaagctt
      541 tcgacgaaat tggcgcacgc cttcaaagct atagtgatcc caatcaggtt gaattctata
      601 cttcgggccg cacttccaat gaagctgcct ttctttatca gctttttgcc cgtgaatacg
      661 ggagcaataa ctttcccgac tgctccaaca tgtgccatga accgacaagc gtgggtttgg
      721 cagcgagtat cggtgtaggt aaagggaccg tgttgctgga agactttgag aagtgcgatt
      781 tagtcatttg cattgggcat aaccctggta caaaccaccc tcgcatgctg acttcgttgc
      841 gcgctttagt gaaacgggga gcgaaaatga tcgccatcaa tcctctacag gaacgtggcc
      901 tggagcgatt taccgcaccg caaaacccgt ttgaaatgct gacgaactct gagactcagt
      961 tggccagtgc ctactataac gtgcgcattg gtggcgatat ggcgttgctc aaggggatga
     1021 tgcgcctgtt aattgagcgc gatgatgctg caagcgccgc aggtcggcct tcattgcttg
     1081 atgacgagtt tattcaaacg cataccgtcg gctttgacga gctacgccgt gacgttctca
     1141 attccgagtg gaaagatatc gaacgtattt ctggactaag tcagacacaa atcgccgaac
     1201 tggctgacgc atatgccgct gccgaacgca ccattatctg ttacggaatg gggatcactc
     1261 agcacgaaca tggtacccag aacgtacagc aactggtcaa tctgctgttg atgaaaggta
     1321 acattggcaa gcctggtgcg ggtatctgcc cactacgtgg acactctaat gtacagggcg
     1381 accgaaccgt cggtatcacc gagaaaccgt ctgcagagtt tctggctcgt ctgggtgagc
     1441 gctatggctt caccccacct catgcacctg gacatgctgc aattgccagc atgcaagcaa
     1501 tatgtacggg gcaggctcga gcattgatct gcatgggggg caattttgcg ctggcaatgc
     1561 cagatcggga agcgagcgct gtaccgttaa cgcaattaga tttggcggta cacgtagcca
     1621 ctaagcttaa ccgctctcat ctgttgaccg cacggcatag ctatattctg ccggttctgg
     1681 gacgtagcga gattgacatg caaaaaaacg gtgcgcaggc ggtaaccgtt gaggattcaa
     1741 tgtcgatgat tcatgcctcg cgtggcatgt taaaacccgc cggtgtaatg ctgaaatcag
     1801 agtgtgcagt ggtcgcggga atcgcgcagg cagcactacc ccagagcgtg gtcgcctggg
     1861 agtatctggt ggaagattat gatcgcattc gcaatgacat tgaagctgtg ctgccagagt
     1921 tcgccgacta taaccaacgc atccgtcatc ccggtggttt tcacctgata aatgcagctg
     1981 ctgaaaggcg ctggatgacg ccgtcaggta aggctaattt cattaccagc aaagggctgt
     2041 tagaagatcc ctcttcagcg tttaacagta agctggtcat ggcgacagta cgcagtcacg
     2101 atcaatacaa cacgacgatt tatggtatgg atgatcgcta tcgaggggta ttcggtcaac
     2161 gagatgtggt ctttatgagt gctaaacaag ctaaaatttg ccgtgtaaaa aacggcgaaa
     2221 gagttaatct tattgcgctt acgccagacg gtaagcgcag ctcacgccgc atggatagat
     2281 taaaagtggt catttaccct atggctgacc gctcactggt gacctatttt ccagaatcga
     2341 atcacatgct aactcttgat aaccacgatc cattaagtgg cattcctggt tataaaagta
     2401 ttccggttga attagaacca tcaaattaat tcgcctcctc atttcttctg ctgtcatccg
     2461 cacagcagaa gaatttctca ttgactatta tcgcgcaatt tgctcacatt gattaaatta
     2521 aaccacacac tataagatat aaacctctgc ctacagctgt aagaaactcc gctcagtact
     2581 gaagtgccag tcctatttcc tcttttctct agcctgttat attaagcata ctgattaacg
     2641 atttttaacg ttatccgcta aataaacata tttgaaatgc atgcgactac agtgaaaaac
     2701 aaaatcacgc aaagagacaa ctataaagaa atcatgtctg taattgtggt tgtcttatta
     2761 ctgaccctta cgttgatagc cattttttcg gcaattgatc agctgggtat ttcagaaatg
     2821 ggtcgcattg caagagatct tacacatttc attatcaata g
//
LOCUS       DBANUU010000294         2841 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000294 DBANUU010000000
VERSION     DBANUU010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.300"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            6..482
                     /gene="agaB"
                     /locus_tag="ACIE68_004811"
     CDS             6..482
                     /gene="agaB"
                     /locus_tag="ACIE68_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFX5910962.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            521..1324
                     /gene="agaC"
                     /locus_tag="ACIE68_004812"
     CDS             521..1324
                     /gene="agaC"
                     /locus_tag="ACIE68_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFX5910963.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1314..2105
                     /gene="agaD"
                     /locus_tag="ACIE68_004813"
     CDS             1314..2105
                     /gene="agaD"
                     /locus_tag="ACIE68_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFX5910964.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2106..>2841
                     /gene="agaI"
                     /locus_tag="ACIE68_004814"
     CDS             2106..>2841
                     /gene="agaI"
                     /locus_tag="ACIE68_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFX5910965.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNF"
BASE COUNT          688 a          673 c          721 g          759 t
ORIGIN      
        1 acacaatgag cagtccaaat attctcttaa cccgtattga taaccgtctg gttcatggcc
       61 aggttggcgt gacctggaca tccaccatcg gtgcaaatct gctggtagtc gtggatgatg
      121 ttgtcgctaa cgatgatatt caacagaaat taatgggtat taccgcggaa acctacggct
      181 ttggcattcg tttctttact atcgaaaaaa ccattaacgt catcggcaaa gctgcaccac
      241 atcagaagat cttcctgatt tgccgtacgc cacaaacggt acgtaaattg gtagaaggtg
      301 gcattgacct gaaagatgtc aacgtcggca atatgcattt ctcggaaggg aaaaagcaaa
      361 tcagcagtaa agtttatgtc gatgaccagg atctcacgga cttacgtttt attaaacaac
      421 gtggcgtgaa tgttttcatt caggatgtcc ctggcgatca aaaagaacaa atccctgact
      481 aaatctaaaa tcgccttaat attggtttga ggtaataaaa atgcatgaaa taaccctact
      541 tcagggatta tccctggcgg cgttagtttt cgttctgggg attgattttt ggctggaagc
      601 cttattttta ttccgcccga taatcgtttg tacccttaca ggtgctattc tcggtgatat
      661 tcagactggc ttaattaccg gtggtctgac agagttggct ttcgccggat taacccctgc
      721 aggtggtgtt cagccgccca acccgattat ggcgggtctg atgaccaccg tcattgcatg
      781 gtctacgggc gttgatgcca aaacagcaat tggtcttggc ctgccgttta gtttgttaat
      841 gcagtacgtc attctgttct tctattccgc tttctcatta tttatgacca aagccgataa
      901 atgcgcgaaa gaggcggata cggcagcgtt ttcccggctt aactggacaa cgatgctcat
      961 cgtcgcttca gcgtatgcgg tgattgcttt cctctgtact tacctggcac aaggggcgat
     1021 gcaggcgctg gtgaaagcga tgcccgcctg gctgacccac ggctttgaag tggctggcgg
     1081 tattctgcct gccgttggtt ttggcttgct gctgcgcgtg atgttcaaag cacaatatat
     1141 cccttacctg atcgccggct tcctgtttgt ttgctacatc caggtcagta acctgttgcc
     1201 ggttgccgta ctgggcgcag gctttgcggt gtatgagttt ttcaatgcga aatcccggca
     1261 gcaagcgcaa ccgcagcccg ttgccagtaa aaatgaagaa gaggactaca gcaatgggat
     1321 ctgaaatcag taaaaaagat atcacccgtc tgggctttcg ttcgtcactg ctgcaagcga
     1381 gctttaacta cgaaaggatg caggcgggcg gttttacctg ggcgatgttg ccgatcctga
     1441 aaaagattta taaggatgac aaaccgggcc taagcgcagc gatgaaagat aacctcgaat
     1501 ttattaatac ccacccgaat ctggtcggat tcctgatggg gttattaatt tcgatggaag
     1561 aaaaaggaga aaaccgcgac accattaaag gcctcaaagt ggcactgttt ggcccaatcg
     1621 ccgggattgg cgatgcgatt ttctggttta ctttgttgcc gattatggcg ggaatttgct
     1681 catcatttgc cagccaggga aacctgctgg ggccgattct atttttcgcc gtttacctgc
     1741 ttatcttttt cctgcgcgtc ggctggaccc acgtcggtta ttcagtcggc gtgaaggcga
     1801 tcgataaagt acgagagaac tcgcagatga ttgcccgttc ggcaaccatc ctcgggatca
     1861 cggtaatcgg cgggctgatc gcttcgtatg tgcatattaa cgtggtgaca tcgtttgcca
     1921 tcgacagtac ccacagcgtc gcgctgcagc aggatttctt cgataaagtc ttcccgaaca
     1981 ttttaccgat ggcctacacc ctgctgatgt attacttcct gcgggtgaaa aaagcgcatc
     2041 cggtgctgtt aatcggcgtg acttttgtgc tctctattgt ttgttccgca ttcggcattt
     2101 tgtaaatgga acgaggcact gcgtctggtg gtgcctcttt acttaaggaa tttcatcctg
     2161 tgcaaaccct tcagcaagtt gaaaactata cggcgttaag tgaacgtgcc agcgaatatt
     2221 tattggctgt gatccgtagc aaaccggatg ccgtgatttg cctggcgact ggagccacgc
     2281 cattactgac gtatcattat ctggtagaaa aaatccatca gcagcaggtt gatgtcagcc
     2341 aactcacctt cgtgaagctc gacgaatggg tggatctgcc attaacgatg cctggcacct
     2401 gcgaaacttt cctgcaacag catatcgtgc agccgctggg gctacgtgaa gaccagctca
     2461 tcagctttcg ctccgaagag ataaatgaga cagagtgcga acgggtaacg aacctgattg
     2521 cgcgcaaagg cggtctggat ttatgcgttc tcggattggg gaaaaacggt catcttgggc
     2581 tgaacgaacc gggagaaagc ctgcaaccgg cctgccatat cagtcaactt gatgccagaa
     2641 cacagcaaca tgagatgtta aaaaccgcgg gtcgccccgt gactcgtggg atcaccttag
     2701 gcctgaagga tattctcaat gcccgcgaag ttttgttact ggtgactggc gaaggaaagc
     2761 aggatgcgac agaacgtttt ctcacggcta aagtctccac cgctatcccg gcttcgtttt
     2821 tatggctgca cagtaacttt a
//
LOCUS       DBANUU010000295         2795 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000295 DBANUU010000000
VERSION     DBANUU010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.301"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>2795
                     /locus_tag="ACIE68_004815"
     CDS             <1..>2795
                     /locus_tag="ACIE68_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFX5910966.1"
                     /translation="GRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFL
                     GAMTEDDARPEALRRFELMVEEVARNASAVAQNTAAAKKSASDAGTSAREAATHATDA
                     AGSARAASTSAGQAASSAQSASSSAGTASTKATEALKSAAAAESSKSAAATSAGAAKT
                     SETNAAASQLSAATSASTATTKASEAATSARDAAASKEAAKSSETNASSSASSAASSA
                     TAAGNSAKAAKTSETNADNSAQAAADSQTASANSATAAKKSETNAKNSEAAAKSSETN
                     AKASETNAKSSETNAAKSAADALNYCNQAQVIVGDNIGLGSAPRDCPDISGNPSGYIG
                     FMRIMSNAKGFPSIASGESSLTGFISQVDGTPAYTGVFQGWATRSLYTYRWNPTTGPQ
                     WTRHARKNEVDRLDQWNSETWLYNHDKSMRLGLTGSTWGCYSDTQKKWIPLDVSHGGT
                     GANSLDDAKTNLQIPEGGLTKAMTLNAPGGAVDGKYYPVIIDTSAMNGSGAMLCPVEI
                     KTAGGPASNPLNSNSFFGFIRCGGWSDVRDVAYGSFVCYEKNELGILCIKASQRDYAQ
                     HVAFYIHKSAFPVVVQTGYKAKVKVPTEDYVIGDNGVRYKFGVSTSTEDNDKNFVKNV
                     LDFTGGEHGFYSNLPFRQGVSERIALTNGLSFDKNFTVTIPIFVNNGQIKATQSFTAY
                     GTDASNRTFVSQRLQSEGGPVVDQTELRAANASGEIIVRDINNSGNSKFFNFNLDGTF
                     SSYQGLVVHTGQNWSTQHTDNVNKFKPIAGSAGGPDGTMVVGGFHAQFSGNYVTQFAG
                     RNSKFWARSFEAGVDKGWKRLLTVDDLNSSTDLAVRSLTTSNPVKSGGGRIDVLGSTS
                     DYSKMDCFVRGFDSTGNSLAWALGSSVGVSKMLSLKNFFSGAEILLNGNDGAVQLKTG
                     AVNGAKAQALTINKDEV"
BASE COUNT          805 a          645 c          719 g          626 t
ORIGIN      
        1 gggcgttaca gcatggacgt tgagtacggt cagtacagcg ttattctgtt ggtggaaggc
       61 ttcccgccat cgcatgccgg gaccatcacc gtgtatgaag actcacaacc gggtacgctg
      121 aatgattttc tcggtgccat gacggaggat gatgcccgtc cggaggcact gcgccgtttt
      181 gaactgatgg tggaagaggt ggcgcgtaac gcgtccgcag tggcacagaa cacggcagcc
      241 gcaaagaagt cagccagcga tgccggcaca tctgcccgtg aggcggcaac ccatgcaacc
      301 gatgctgcag gctcagcacg tgccgccagc acgtcagccg gacaggctgc atcatctgct
      361 cagtcagcat cttccagcgc aggaacggca tcaacaaagg ccactgaagc attaaaaagt
      421 gctgccgctg cagagtcctc aaaaagcgcg gcagccacca gtgccggtgc ggcgaaaacg
      481 tcagaaacga atgccgcagc atcacaacta tcagcagcca cttctgcatc caccgcgacc
      541 acgaaggcgt cagaagctgc cacctcagcc cgggatgcgg cggcctcaaa agaggcagcg
      601 aaatcatcag aaacgaacgc atcatcaagt gccagtagtg cagcttcctc ggcaacggcg
      661 gcaggaaatt ccgcgaaggc cgcaaaaacg tctgagacga atgcggataa cagcgcacag
      721 gcggcagcag actcacaaac tgcatcggca aactccgcga cagcagccaa aaaatcagaa
      781 accaacgcga aaaatagcga ggcagcagca aagagcagcg aaacaaacgc taaagccagc
      841 gaaactaatg ctaaatccag tgaaacaaac gcggcgaaat ctgcggcgga cgcacttaat
      901 tattgcaacc aggcgcaagt aattgttggc gataatatcg gtcttggctc agccccgcgt
      961 gattgtcctg atatttccgg caacccatca gggtatatcg gatttatgcg cattatgagt
     1021 aatgcaaaag gctttccatc gattgcatcc ggtgaaagca gtcttacggg gtttattagt
     1081 caggtagatg gaacaccagc gtatacaggt gtatttcagg gatgggctac gcgctcgctt
     1141 tatacttatc gctggaatcc gacaacaggc ccgcaatgga cacgccacgc tcgcaaaaat
     1201 gaagtggacc gtcttgacca atggaatagc gaaacatggt tatataacca tgataaatcc
     1261 atgcgcctgg gtttaaccgg atcaacatgg gggtgttaca gcgacacaca aaaaaaatgg
     1321 ataccgcttg atgtttctca tggtggcact ggcgcaaata gccttgatga cgccaaaact
     1381 aatttgcaaa tccctgaggg tggattaaca aaagcgatga cccttaatgc ccctggtggt
     1441 gcagtggatg gcaaatacta ccctgtcata atagacacat ctgcaatgaa tgggagcggt
     1501 gccatgttat gtcctgttga aataaaaaca gcaggaggtc cggcatcaaa tccgcttaat
     1561 agtaactcat tttttgggtt cattcgatgt ggtggctgga gtgacgttcg cgatgtggct
     1621 tacgggtcat ttgtttgcta tgaaaaaaat gaactcggga tactttgcat taaagcgtcg
     1681 caaagagatt atgcgcagca tgtagctttc tacatccata aaagcgcttt tcctgtcgtt
     1741 gtgcaaactg gctacaaggc gaaagttaag gttccaacag aagactacgt aattggtgac
     1801 aacggggtta gatataagtt tggtgtatca acgtcaacgg aggataacga caagaacttt
     1861 gttaaaaatg ttcttgactt cactggcggt gaacatggct tctacagcaa ccttccgttc
     1921 agacaagggg tgtcagaaag gattgcatta acaaatggcc tgagttttga caaaaatttt
     1981 accgtcacta ttcctatatt tgtaaacaat ggtcagatta aagcaacaca atctttcacc
     2041 gcgtacggta cagacgcttc aaacagaaca tttgtttctc aaagattaca atccgaagga
     2101 ggtccggttg tcgatcaaac tgaactaagg gccgctaacg cttctggtga aattattgtc
     2161 agggacataa ataattcagg gaacagtaag ttctttaatt ttaaccttga tggtaccttc
     2221 agttcttacc agggattggt tgttcacaca gggcaaaact ggagcactca acatacagat
     2281 aatgtaaata agtttaagcc aatagcaggg agcgcaggcg gtcctgacgg aacaatggtt
     2341 gttggcggat tccatgctca atttagcggt aattacgtca cacaattcgc cggtcgcaac
     2401 tccaaatttt gggcaagaag ctttgaggct ggggttgata agggatggaa acgactatta
     2461 acagtagacg atctcaattc atctaccgat cttgctgtca ggtcattaac cacatctaac
     2521 ccggtaaaat ctggcggagg gcgaattgat gtccttggaa gcacgtcaga ctatagcaaa
     2581 atggattgct ttgtacgtgg gtttgatagc accggtaatt ctctcgcgtg ggcgttgggt
     2641 tcatcagtcg gcgtaagtaa gatgctgtcg ctaaaaaatt tctttagcgg agctgagata
     2701 ctgttaaatg gtaatgacgg cgcggttcaa ctcaaaacag gtgctgttaa cggggctaaa
     2761 gcgcaggcgc tcactatcaa caaggatgag gttaa
//
LOCUS       DBANUU010000296         2790 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000296 DBANUU010000000
VERSION     DBANUU010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.302"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..125)
                     /locus_tag="ACIE68_004816"
     CDS             complement(<1..125)
                     /locus_tag="ACIE68_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001359473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFX5910967.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIR"
     gene            complement(155..766)
                     /locus_tag="ACIE68_004817"
                     /pseudo
     CDS             complement(155..766)
                     /locus_tag="ACIE68_004817"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            complement(771..1469)
                     /locus_tag="ACIE68_004818"
     CDS             complement(771..1469)
                     /locus_tag="ACIE68_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFX5910968.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQND
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTFDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(1469..1798)
                     /locus_tag="ACIE68_004819"
     CDS             complement(1469..1798)
                     /locus_tag="ACIE68_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFX5910969.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPRWEATALESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(1795..>2790)
                     /locus_tag="ACIE68_004820"
     CDS             complement(1795..>2790)
                     /locus_tag="ACIE68_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFX5910970.1"
                     /translation="AQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLTSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSLADSRSQAS
                     GTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
BASE COUNT          564 a          929 c          690 g          607 t
ORIGIN      
        1 cggatggctt cagcccccgt tttcacacga aggtcgatgc ggcggccaaa tcgttgcaaa
       61 tccccgtaaa ggcagatgcg cgccatgccc ggtgacgcca gagggagtgt gtgcgtcgct
      121 gccatttgtc ggtatacctc tctcgtttgc tcagttgttc aggaatatgg tgcagcagct
      181 cgccatcacc acagtaaatg gcggcatgat tcggcaccga tgaaccaaaa cagcacagca
      241 gcacatcgcc cggttgtgct gatgacaacg gcacctgata cagccctgtg gcctccagat
      301 tatccagata gagattctgg ccgttacgcc accagtcatc ctcacgatga aagtccggca
      361 tctcaatccc cgccagatga taagcatccc ggaacagcgt gtaacagtcc gtcaccccgt
      421 gctcaaagcg ccgcccggtg agatgcggca cacagcggaa cttatgaatc gtcccccggc
      481 agaccagcca ccacggcaaa tcactctgca cctgcagccg ccggtcggcc tcactcagcc
      541 agggcagacc accggggtgg ctgtggacca gcgccacaat ctcaccctgc atttctgcct
      601 gcagccagtc ctccggagcc atccggaaat aatcctccgg ctcaccggaa atattcacgc
      661 agggaaaata tctttccccc tccggcgttc tcaccacgaa gccgcacgac tccgctggcg
      721 cacatcgccg ggcgtgcgcc agaatcgctg attctgtctc tgtcatggga ttactgcgaa
      781 agtttgttaa tggaaaggaa gccgccaaag ttgccgacgt tattgcgaaa cttacagcca
      841 ctcaggcatt tgctgcattt atccttcgtg atatcgaacg ttggctggtc atattcatcc
      901 gcgaccgccg gaccgtgata accgcactca tcgccgcgat aggtccaggt gcaggtgttg
      961 gccagcatga tacgtcccgg aaaaacagcg ccgtccgttt ccgtcggcgt ggacagtaca
     1021 aaagaggcac tcaccgcgct cagttcgctg cactgctcaa tgcgccagcg gctgatcacc
     1081 tcctgctccg gatcggcgtc actgtttccg ttgacgaagt tcaccgcatc cagaaaacgg
     1141 gcgtaaacct tacgccggac caccgttccg ccgaccagac tctgcagatc ttccgccatc
     1201 ccggtgacca tgccgtgcag gttagaaacc gtcagtgtcg gacgggcagc actgcctttg
     1261 ccgttcagtt caaatcccgt cccctgaatg ggatacgcct gatactgtcg cccctgccag
     1321 gtgaccggct cacctttatc gttctgctca ttacagaaaa aataacgttc tccaccgacc
     1381 tctgtcaggt cgatttccca gagcaccacg ctggccgact gctccgcacg ggtgcattca
     1441 ttcagtgttt cctgtcggat atcctgcatc agttcaccac ctgttcaaac tctgcgctga
     1501 actcaacacg cagcatactg acccgcgacg accattttgc gcaggtcacc tttatctgcc
     1561 gccactcata aggcggcgtc cacagaaagg ctttccagcc cccgtgctct tccagaaacg
     1621 actccagcgc cgtggcctcc caacggggga cagaaagcgt cacgctgtac gttttcaggt
     1681 tggcattcag cccggcaggc gctcgctggg aatagccatc accaaagcgc acctttctta
     1741 cagaaggggc cgaagccaca tccataccgg gtttcacttt ccagcggaag gttttcatcg
     1801 tccacctccg gagaacagtc caccatcacg catctgtgtc tgaatttcat cacgggcacc
     1861 cttgcgggcc atgtcataca ccgccttcag agcagccgga cctatctgcc cgttcgtgcc
     1921 gtcgttgtta atcaccacat ggttattctg ctcaaacgtc ccggacgcct gcgatcggct
     1981 gtcagccaga ctgcccggtg taccgacata accaccggtg gcatagccgc gcatcagccg
     2041 gtagagattc cccacgccaa tccggctggt tgcctccttt gtgaagacaa attcaccacg
     2101 gtgaacaatc cccgctggct catatttgcc gccggttccc gtaaatcctc cggttgcaaa
     2161 atggaatttc gccgcagcgg cctgaatggc tgtaccgcct gacgcggatg cgccgccacc
     2221 aacagccccg ccaatggcgc tgccgatact cccgacaatc cccaccattg cctgcttaag
     2281 cagaatttct gtcatcatgg acagcacgga acgggtgaag ctgcgccagt tctgctcact
     2341 gccggtcagc atcgccgcca tattctgtgc aataccatca aaggtctgcg tggctgcact
     2401 ttttacctgc gacatactgt ccgtggcgct ctcttcccac tcactccagc cggacgtcag
     2461 gcctgccatc cagctcccgc gaagctggtc ttcagccgcc caggtctttt tctgctctga
     2521 catgacgtta ttcagcgcca gcggattatc gccatactgt tccttcaggc gctgttccgt
     2581 ggcttcccgc gctgcctgcc ggtcagtcag cccccggctt ttcgcatcaa tggcggcccg
     2641 ttttgcccgt tgctgctgtg cgaatttatc cgcctgctgc gccagcgcgt tcaggcgctc
     2701 ctgatacgta accttgtcgc caagtgcagc cagctggcgt ttgtactcca gcgtctcatc
     2761 tttatgcgcc agcagggatt tctcctgtgc
//
LOCUS       DBANUU010000297         2764 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000297 DBANUU010000000
VERSION     DBANUU010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.303"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(107..>2764)
                     /gene="chiA"
                     /locus_tag="ACIE68_004821"
     CDS             complement(107..>2764)
                     /gene="chiA"
                     /locus_tag="ACIE68_004821"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HFX5910971.1"
                     /translation="ALPALAMEAWNNQQGGNKYQVIFDGKIYENAWWVSSTNCPGKAK
                     ANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSNTPASNTPANGGSATPAQG
                     TVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNCPGDAKSNDASNPWRYVRA
                     ATATEISETSNPQSCTSAPQPSPDVKPAPDVKPAPDVKPAPDVQPAPVDKSNDNYAVV
                     AWKGQEGSSTWYVIYNGGIYKNAWWVGAANCPGDAKENDASNPWRYVRAATATEISQY
                     GNPGSCSVKPDNNGGAVTPVDPTPETPVTPTPDNNEPSTPADSGNDYSLQTWSGQEGS
                     EIYHVIFNGNVYKNAWWVGSKDCPRGTSAENSNNPWRLVRTATAAELSQYGNPTTCEI
                     DNGGVIVADGFQASKAYSADSIVDYNDAHYKTSVDQDAWGFVPGGDNPWKKYEPAKAW
                     SASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNSRPWKPLGKAQSYSNE
                     ELNNAPQFNPETLYASDTLIRFNGVNYISQSKVQKVSPSDSNPWRVFVDWTGTKERVG
                     TPKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVVSKDANTCLPTWGTAY
                     GMQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDLMQHYYDIVDNLNLKV
                     LDFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTLPILPTGLTPEGMNVL
                     SDAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSAIANLHSQLKGLHPNK
                     SDAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLGMVGIWSIARDLPGGT
                     NLSPEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT          652 a          724 c          656 g          732 t
ORIGIN      
        1 gactcttttt taagcaaaga atagaaatat gaataaaaat aatgggctac caaaagtagc
       61 ccattgacaa aaaatgcggc gatactggaa ggtatcgcca acacatttat tgcttagtaa
      121 acggcgcgaa gatttcgcta aatgcgtact tcggtgcctg ttctttagtc aggccgtgga
      181 attccggaga caggttagtg ccgcccggca ggtcgcgcgc gattgaccag ataccaacca
      241 taccgagatt acgcttctgc gcatcctgca tgaccagacg agcatcagag agatagaaca
      301 cctcgccctg aacgtcgttc acgccaacca tcggcgtggt acccatcata gcgtcaattt
      361 ctgcatcgct cttattggga tggaggcctt tcaattgtga atgcaggttg gcaatcgcag
      421 acgttgcaca cttaccgtga atgttctggc cttcggtatt tgcagactga caaatcgcgt
      481 taccgtagtc cattgtcatc acgttcacac ccgccagctc aacacctttc gctttggcat
      541 cgctcaggac attcatccct tccggcgtca ggccagtcgg cagaattggc aaggtgtacc
      601 agatagcaat atctttgcct tctgacttcc atttatcctg cactttcttc acagcaaggt
      661 tacgacgttc aatagatgcc tgatccgcaa cccaggtgcc ttcgatatcg aagtccagga
      721 ctttgaggtt caggttatca acgatgtcat aataatgctg catcagatcg tctacgttct
      781 tacaggaagc agccagcgga gcgttgttag caccaccgat agacagcatc acatcgccgc
      841 cagcctcacg cagagctttg attttgctgt actgagcgta attctgcata ccataagcgg
      901 taccccatgt cggcagacag gtgttcgcat ctttactcac cacaaacgcc agcgtgaagt
      961 ggttgacgtt atgattctta gccagcgcag ccaaatccgg gatcgtattc agcgtaaagt
     1021 caacatacgg tgcataaacg tgtttcggcc atgctttctt cggcgtacct acacgttctt
     1081 tggttccggt ccagtcaaca aaaacacgcc acgggttgct gtcagaagga gaaactttct
     1141 gcactttact ctgagaaatg tagttcacac cgttaaagcg aatcagcgta tcgctggcat
     1201 aaagcgtttc tggattaaac tgcggcgcat tattcagctc ttcgttgcta tagctctgag
     1261 ctttacctaa cggcttccac gggcggctat tgctaccggt ggcgttttgg ttcgcaacca
     1321 gagcagggtt gtcactttgc gtccagaaca gcgcttcata agcctgccca tcaacaacaa
     1381 cgcgatcacc tttcacgtac acagtggatg cggaccatgc tttcgccggt tcgtatttct
     1441 tccacgggtt atcgccgccc gggacaaagc cccatgcgtc ttgatcgaca gaagttttat
     1501 aatgtgcatc gttataatct acgatgctgt ccgcgctgta cgctttgctg gcctggaaac
     1561 catccgcaac aatgacgccg ccgttatcaa tttcacaggt agtcgggtta ccgtactgac
     1621 tcaattccgc agcggtagct gtacgcacga gacgccatgg gttattggag ttttcagcgc
     1681 tggtaccccg tgggcaatct ttagacccaa cccaccaggc gttcttgtaa acattaccat
     1741 tgaaaataac atggtaaatt tcgctacctt cctggccgct ccacgtttgc aatgagtaat
     1801 cgttaccgct atccgctggt gttgatggct cgttgttatc cggggttggg gtcaccggtg
     1861 tttccggagt tggatcaacc ggagtcacag caccgccatt attatccggc ttaacggaac
     1921 aggagccagg gttaccatac tggctgattt ctgttgccgt tgccgcgcga acataacgcc
     1981 atgggttact ggcatcgttt tctttcgcat cgcctgggca atttgccgcg cctacccacc
     2041 aggcgttctt ataaatgccg ccgttataga taacgtacca tgtagaagaa ccttcctgac
     2101 ctttccaggc tactacagca tagttgtcgt ttgacttatc aactggggct ggctgaacat
     2161 caggagccgg tttaacgtcc ggtgccggtt tcacatccgg tgccggtttc acatccggtg
     2221 aaggctgtgg tgctgaagta caggactgtg gattactggt ttctgagatt tccgttgccg
     2281 tagcggcacg aacataacgc catgggttgc tggcatcatt gcttttcgca tcacccggac
     2341 agttagaaga ggctacccac caggcatttt tatataccgc accattaaag acgacatacc
     2401 aggtctgacc gccctgctgt ttattccagg caactacaga agaattagac ggaacagtgc
     2461 cctgtgctgg tgtagccgaa ccgccattag ccggcgtatt ggatgcaggc gtatttgaat
     2521 ttgaagaaga tgagctgccg ctcttttcgc aggaaagtgt attgccaaac tgactaattt
     2581 cagcagctgt tgcggtacgc tttaaacgcc acgggttagt tgcatcattt gctttcgctt
     2641 ttcccgggca atttgtagaa gaaacccacc aggcattttc ataaattttg ccatcgaaaa
     2701 taacctgata tttattacca ccttgttggt tattccatgc ttccattgcc aatgctggca
     2761 gagc
//
LOCUS       DBANUU010000298         2732 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000298 DBANUU010000000
VERSION     DBANUU010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.304"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(29..1600)
                     /locus_tag="ACIE68_004822"
     CDS             complement(29..1600)
                     /locus_tag="ACIE68_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFX5910972.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQELISALQEEMAETLGEQYDPVLPS
                     PLRQSSARKPLPASLPRETRVIRPEEECCPACSGELSPLGCDVSEQLELISSAFKVIE
                     TQRPKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPAVWFAYSPDRKGVHPQNHLAGYSGVLQADAYGGYRVLYESG
                     RITEAACMAHARRKIHDVHARMPTDITTEALQRIGELYAIEAEVRGCTAEQRLAARKA
                     RAAPLMQSLYDWIQTQMKTLSRHSDTAKAFAYLLKQWDGLNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWLFAGSDSGGEHAVVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLLPWKVDLTSQ"
     gene            complement(1620..1967)
                     /gene="tnpB"
                     /locus_tag="ACIE68_004823"
     CDS             complement(1620..1967)
                     /gene="tnpB"
                     /locus_tag="ACIE68_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFX5910973.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWSVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(1967..2617)
                     /gene="tnpA"
                     /locus_tag="ACIE68_004824"
     CDS             complement(1967..2617)
                     /gene="tnpA"
                     /locus_tag="ACIE68_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFX5910974.1"
                     /translation="MNARKAVLADNPELILRVLQLRFDESLSYPRISAQTGVSKTAIF
                     SLVRRFHQIFADWPLSGEYSCMQLARALFPGRYPSAPTVTQPVKTEKIRRNRFSPEFK
                     WRLVQQTLLPGACVAQIARENGINDNLLFNWRHLWRNGGLQPPGEHETSLLPVTLTPE
                     PDNKIPAPVQIPEQINTLPDSLCCELVLPAGTLRLKGELTPALLQTLIREMKGSSH"
BASE COUNT          574 a          755 c          756 g          647 t
ORIGIN      
        1 aagcggctcg ccagaaccgt attgatattt actgagaggt cagatcaact ttccagggca
       61 acagatcgcg tacccggttt gccggccagt cctggatatg ctcaatgacg taacgcagcc
      121 atttttctgg ttccacattg ttcagacggc atgtgccgat cagcgagtac aacaccaccg
      181 catgttcgcc accgctgtcg gaacccgcga agagccagtt tttccggcct acggccactc
      241 cccgtaaggc gttctctgcg atgttgttgt cgatttccac ccagccatta ctgcagtaca
      301 cgttcaggcc atcccactgt ttcagcaggt atgcgaacgc ttttgccgta tccgagtgac
      361 gcgacagtgt tttcatctga gtctgtatcc agtcatacag tgactgcatc agtggcgcag
      421 ctctggcttt tcttgccgcc agacgctgtt ctgctgtaca tccccggact tctgcctcta
      481 tggcatacag ttcaccgata cgctgcaggg cttccgtggt gatgtcggtg ggcattcttg
      541 catgcacatc gtggattttt ctccgggcat gagccataca cgcggcttcc gttattctgc
      601 cggattcgta taacacccgg taaccaccgt aagcatcggc ctgaagcaca ccgctgtaac
      661 cggccagatg attctgtgga tggacgcctt tccggtccgg gctgtacgcg aaccagaccg
      721 ccggaggcat ctgtgaaccg gcgttacggt catcacggac gtagacccac agccgggcag
      781 tccttgtttt accactgccc ggctcctgga ccgggacggg gatatcatcg gcatggactt
      841 taccgggcat cagcacatac tggcgcagga cgtcatacag cggctccagc agttcagcaa
      901 cggcacctgt ccagcgcccc agcgtggcgc ggctcagctc cacgccctgg cgacggtata
      961 tttctgactg gcggtataac ggcagatggt ctgcatattt cccggtgaca acatgggcca
     1021 gaagccccgc tccggcataa ctgcgtgcaa tgggttttga aggcaccggt gcctgcacga
     1081 tatggtcgca ccggcaacag gcctgtttcg gacgttgtgt ttcgataacc ttaaaggcac
     1141 tgctgataag ctccagttgc tctgacacat cacatcccag tggactgagt tcaccactac
     1201 aggccggaca gcattcctct tccggccgga tgacccgggt ttcacgggga agtgaagccg
     1261 gtaacggttt acgggctgaa gactggcgca ggggggatgg cagtaccggg tcatattgct
     1321 cacccagcgt ttctgccatt tcttcctgaa gtgcgctgat tagctcctgt gcttcctgta
     1381 tctgccgttc ggtttttgcg cgaagttttt ctgagctttt accgaactgc atacgctgca
     1441 gtttcgcaac cagcgccttc agccggttga tttcggaagc ataagccgcc acccgctgtg
     1501 agagcaggcg gttgtattcg gccatctggc ggatggtgtc ctgttgcgtc tgcaacagtg
     1561 cccgcaggcg ggcattctca tgagcaagtg aggtgtccat atcctcactt tacaacgggt
     1621 tatatacgga ttccagcgcg ttccgttcgt ttcgggtgct tccagtcgat gccctcgaga
     1681 agcatggaca actgagccgg agtaaggtga acctttccat cgcgggtgac cgaccagacg
     1741 aagcggcccc gctccaggcg tttggtgaag aggcacagtc cgtcactgtc agcccacagc
     1801 acttttatct ggtcaccccg gcgtccgcgg aagatgaaca gatgcccgga gaacgggtca
     1861 tccttcagga cgttctgaac ttttgaggcc aggccgttaa agccatttcg catatcggtg
     1921 ataccggcaa ccagccagat acgcgaaccg gcagggagag atatcatcag tggctgctcc
     1981 ctttcatttc gcggataagt gtctgtaata acgccggtgt cagttcacct ttcagcctga
     2041 gagttccggc cggcagaacc agctcgcagc acagactgtc tggcagtgta tttatctgtt
     2101 caggtatctg cactggtgcc gggattttat tatccggctc cggcgttaac gtcacgggaa
     2161 gtagcgatgt ttcatgttcg ccgggcggct gcaggccgcc gttacgccag agatgccgcc
     2221 agttaaagag caggttatcg ttgattccgt tctcgcgagc tatttgtgcg acacaggcac
     2281 cgggtaaaag agtttgctga acaagcctcc atttaaactc cggtgaaaat cggttccggc
     2341 ggattttctc tgttttcaca ggctgagtca ctgtcggggc tgaagggtat cgcccaggga
     2401 aaagagcccg ggccagttgc atgcaggaat attcaccgga aagaggccag tcagcgaata
     2461 tctggtgaaa tcgcctcacc agagaaaaaa tggcggtttt gctgactccg gtctgcgcag
     2521 aaatgcgcgg gtacgacagt gactcgtcaa atctcagctg tagcacacgc aggatcaatt
     2581 ccggattatc tgccagtacc gcctttcgtg cattcatctt aaatgtcctt ttactgcgaa
     2641 aatggacatt agtatcggaa acaggaaagg gaggcgaaag acggtttaaa tgatgcgctt
     2701 acgcatgaat ggatggtgga gaagaatggt ac
//
LOCUS       DBANUU010000299         2723 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000299 DBANUU010000000
VERSION     DBANUU010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.305"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            288..1160
                     /locus_tag="ACIE68_004825"
     CDS             288..1160
                     /locus_tag="ACIE68_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFX5910975.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDDYFWRHILHRGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1531..>2723
                     /locus_tag="ACIE68_004826"
     CDS             1531..>2723
                     /locus_tag="ACIE68_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFX5910976.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAIKSGAVATDTVVNTGAEGGPDAENGDTGQTVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IVKNGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSG
                     AAVSGT"
BASE COUNT          649 a          716 c          767 g          591 t
ORIGIN      
        1 ccacaataca ctaaaaaata ctcacagcag taaacacaga cacacctgat aatcatacag
       61 ccgataaata ttttcccctt tacggagaaa tcagcctgct gtttaacaat aacggcgtat
      121 tttcatggaa ggagatcacc cagtaacgga taagtcctga tctccttcat gttaatacgc
      181 aacaatccca tcgattcaaa acaatttcag acagatatta tctccgtgaa tcccccgcca
      241 cctttccggt gcgcggggtt ttgtcttttt tcaccgggaa tacatgtatg aatccgtctg
      301 atgccattga ggcaattgaa aaaccgctct cctccctgcc ttactcactt tcccgtcaca
      361 tcctggaaca tctgcgtaaa ctcaccagtc acgaacccgt gattggcatt atgggtaaaa
      421 gcggggccgg taaatcctca ctctgtaatg cactgtttca gggggaggtc accccggtca
      481 gtgatgttca cgccggcacc cgggaagtac ggcgcttccg tctgagtggc cacggtcaca
      541 gcatggttat cactgacctg cccggggtgg gcgagagccg ggacagggat gcagagtatg
      601 aggccctgta ccgtgacatt ctgcctgaac tggacctggt actgtggctg attaaagccg
      661 atgaccgtgc cctgtctgtg gatgattatt tctggcgaca catcctgcac cggggacatc
      721 agcgggtact gtttgtggtg acgcaggccg acaaaacgga gccctgccat gaatgggata
      781 tggccggtat tcagccttct cctgcacagg cacagaatat tcgcgaaaaa acggaggcgg
      841 tattccgtct gttccggccc gtacatccgg ttgtggccgt atcggcccgc accggctggg
      901 aactggatac gctggtcagt gcgctcatga cagcgcttcc cgaccatgcc gccagtcccc
      961 tgatgacccg actgcaggac gagctgcgca cggaatctgt ccgctctcag gcccgtgaac
     1021 agtttaccgg tgcggtggac cggatatttg acacagcgga gagcgtctgt gttgcctctg
     1081 tggctcgtac ggttctgcgt gccgtccgtg acacggtggt ctctgttgcc cgcgctgtat
     1141 ggaactggat tttcttctga acactctgcc aggggaaatg cctgattaca ttgcgttaac
     1201 caactgaaaa catgcccccc cccctcatac ccacctcttt ctcttgcgtg acttccctgc
     1261 cgttaataga ataaaacgat cgataaaaca ggtatcgatc gttactatcg atcgtttata
     1321 gtggttaatg gtctgcgaaa aggcaactga atctcttcat catgcagaac ggaattgcac
     1381 acaacggact gatacttctc tggcggatga gagaggggaa gcatttatgt ccgggaaaaa
     1441 ccactttatc tgaccatgct gttttgtacc tgccggtatc cacatttgtg ggtaccggct
     1501 tttttattca ccctctgtaa ggaaaagctg atgaaacgac atctgaacac cagctacagg
     1561 ctggtatgga atcacattac gggcaccctg gtggtggcct ccgaactggc ccgctcacgg
     1621 ggaaaacgcg ctggtgtggc ggttgcgctg tctcttgctg ctgtcacatc agtcccggca
     1681 ctggctgctg acaaggttgt acaggcggga gaaaccgtga acgacggaac actgacaaat
     1741 catgacaacc agattgtcct cggtacggcc aacggaatga ccatcagtac cggtctggag
     1801 tatgggccgg ataacgaggc caataccggc ggacaatgga tacaaaatgg cggtatcgcc
     1861 aacaacacta ctgtcaccgg tggtggtctt cagagagtga atgccggagg aagcgtttca
     1921 gacacggtta tcagtgccgg aggcggacag agccttcagg ggcaggcagt gaacaccact
     1981 ctgaacggcg gtgagcagtg ggtacatgaa ggcgggattg caacgggtac cgtcattaat
     2041 gagaagggct ggcaggccat caaatccggc gcagtggcaa ccgacacggt tgtgaatacc
     2101 ggcgcggaag ggggaccgga tgcggaaaat ggtgataccg ggcagaccgt ctacggagat
     2161 gccgtacgca ccaccatcaa taaaaatggt cgtcagattg tggctgctga aggaacggca
     2221 aataccactg tggtttatgc cggcggcgac cagactgtac atggtcacgc actggatacc
     2281 acgctgaatg gggggtacca gtatgtgcac aacggcggta cggcgtctgg cactgttgta
     2341 aacagtgacg gctggcagat tgtcaaaaac gggggggtgg ccgggaatac caccgttaat
     2401 cagaagggca gactgcaggt ggacgccggt ggtacagcca cgaatgtcac cctgaagcag
     2461 ggcggcgcac tggttaccag tacggctgca accgttaccg gcataaaccg cctgggagca
     2521 ttctctgttg tggagggtaa agctgataat gtcgtactgg aaaatggcgg acgcctggat
     2581 gtgctgaccg gacacacagc cactaatacc cgcgtggatg atggcggaac gctggatgtc
     2641 cgcaacggtg gcaccgccac caccgtatcc atgggaaatg gcggtgtact gctggccgat
     2701 tccggtgccg ctgtcagtgg tac
//
LOCUS       DBANUU010000300         2721 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000300 DBANUU010000000
VERSION     DBANUU010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.306"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            257..505
                     /locus_tag="ACIE68_004827"
     CDS             257..505
                     /locus_tag="ACIE68_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFX5910977.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            750..824
                     /gene="tap"
                     /locus_tag="ACIE68_004828"
     CDS             750..824
                     /gene="tap"
                     /locus_tag="ACIE68_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFX5910978.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            817..1674
                     /gene="repA"
                     /locus_tag="ACIE68_004829"
     CDS             817..1674
                     /gene="repA"
                     /locus_tag="ACIE68_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFX5910979.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTLALFAALDV
                     SEDAVAAARRSRVEWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFTANGEAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT          708 a          714 c          674 g          625 t
ORIGIN      
        1 ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg aaacgggaaa
       61 aagcaagttt tccccgctcc cggcgtttcc ataactgaaa acctactatt tcacagttta
      121 aatcacatta aacgacagta atccccgttg atttgtgcgc caacacagat cttcgtcaca
      181 attctcaagt cgctgatttc aaaaaactgt agtatcctct gcgaaacgat ccctgtttga
      241 gtattgagga ggcgagatgt cgcagacaga aaatgcagtg acttcctcat caggtacaaa
      301 gcgagcatac agaaaaggta atcctctgac gcttgcagaa cgtcagcagg catccctggc
      361 aagaaaacga gctacacata aagaactcag agtgttcatt cctgctgcgt taaaagcgca
      421 attgcaggtg atgtgtgaag ctgagggcgt tacccaggct gagatgattg ccgaactgat
      481 aaagcagaag agtgctttta gctaactaaa atgtagcgac atcacattct tgcctgtttc
      541 tggtgtgcgt gatagattgc tgatcgttta aggaattttg tggctggcca cgccgtaagg
      601 tggcagggaa ctggttctga tgtggattta caggagccag aaaagtgaaa accccgataa
      661 tcttcgacaa gtttggcgac tgagaagatt accggggcca tctaaaaacc gtatagccaa
      721 caattcagct atgcggggag tatagttata tgcccggaaa agttcaagac ttctttctgt
      781 gctcactcct tctgcgcatt gtaagtgcag gatggtgtga ctgatcttca acaaacgtat
      841 taccgccagg taaagaaccc gaatccggtg tttacacccc gtaaaggtgc cggaacgctg
      901 aagttctgcg aaaaactgat ggaaaaggcg gtgggcttca cttcccgttt tgatttcgcc
      961 attcatgtgg cgcatgcccg ttcgcgtggt ctgcgtcgac gcatgccacc ggtgctgcgt
     1021 cgacgggcta ttgatgcgct cctgcagggg ctgtgtttcc actatgaccc gctggccaac
     1081 cgcgtccagt gctccatcac cacgctggcc attgagtgcg gactggcgac ggagtctgct
     1141 gccggaaaac tctccatcac ccgtgccacc cgtgccctga cgttcctgtc agagctgggg
     1201 ttgattacct accagacgga atatgacccg cttatcgggt gctacattcc gaccgacatc
     1261 acgttcacac tggctctgtt tgctgccctt gatgtgtctg aggatgcagt ggcagctgcg
     1321 cgccgcagtc gtgttgaatg ggaaaacaaa cagcgcaaaa agcaggggct ggataccctg
     1381 ggtatggatg aactgatagc gaaagcctgg cgttttgtgc gtgagcgttt ccgcagttac
     1441 cagacagagc ttaagtcccg tggaataaag cgtgcccgtg cgcgtcgtga tgcgaacagg
     1501 gaacgtcagg atatcgtcac cctggtgaaa cggcagctga cgcgtgaaat ctcggaagga
     1561 cgcttcactg ctaatggtga ggcggtaaaa cgcgaagtgg agcgtcgtgt gaaggagcgc
     1621 atgattctgt ctcgtaaccg taattacagc cggctggcca cagcttctcc ctgaaagtga
     1681 tctcctcaga ataatccggc ccgcgccgga ggcatccgca cgcctgaagc ccgtcagtgc
     1741 acaaaaaatc agcactacat acaaaaaaca acctcaccat ccaccttctg gagcatccgg
     1801 ttccccctat ttttaataca aaacacgcct cacagacggg gaattttgct tatccacatt
     1861 aaactgcaag ggacttcccc ataagattac aaccgttcat gtcataaagc gccagccgcc
     1921 agtcttacag ggtgcaatgt atcttttaaa cacctgttta tatctccttt aaactactta
     1981 attacattca tttaaaaaga aaacctattc actgcctgtc ctgtggacag acagatatgc
     2041 acctcccacc gcaagcggcg ggccccaacc ggagtcactt tagttacaac actcagacac
     2101 aaccaccaga aaaacccctg tcctgcgcag aactgaagcc acaaagcccc tccctcataa
     2161 ctgaaaagcg ccgccgcccc ggcccaaagg gccggaacag agtcgctttt aattatgaat
     2221 gttgtaacta tactccatca tggctgttag tcttctggct ggaagtattg agaacacgct
     2281 cgtaagcggc cctaacggcc cgctaacgcg gagatacgcc ccgacttcgg gtaaaccctc
     2341 gtcgggacca ctccgaccgc gcacagaagc tctctcatgg ctgaaaacgg gtatggtctg
     2401 gcagggctgg gggggataag gtgaaaccta tcaatacgta ccggctcacg ccgggcttcg
     2461 gcggttttac tcttgtgcca tatgtagcta catcatgccg ccttccatgc cgctggcgcg
     2521 gcatcaaaaa ggggtctgct tggagatcgg aaaattttgt acgttaagca caacaaactt
     2581 aatgctaatg tatgcataca ttagcattaa ccgcacatta aaaacttaac caataacgat
     2641 gcgtattatt acataatatt atcaatcgct agccccagcg accgaacgtt agtgagcgag
     2701 tgggcgagtg ggcgagtggg c
//
LOCUS       DBANUU010000301         2681 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000301 DBANUU010000000
VERSION     DBANUU010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.307"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..374
                     /locus_tag="ACIE68_004830"
     CDS             <1..374
                     /locus_tag="ACIE68_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFX5910980.1"
                     /translation="RYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAPELMGYSELT
                     AIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAASVDDSREKF
                     TPAQNMIFSRKSAQLASHQSV"
     gene            642..2255
                     /locus_tag="ACIE68_004831"
     CDS             642..2255
                     /locus_tag="ACIE68_004831"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFX5910981.1"
                     /translation="MNCKTIILRFRDLVTPAGETITLHQDIIKSKGSVWWGWWAKADE
                     QCPREFNDLKDQISENNPLEIYLFDSGQLKIYFANLIGISTNFDKHPCPVHDMTPPYY
                     SDQQYNVWFNFSSIEEVSDCSGLINGLAYSGAVKDFFKNNDMFQIYSGKQISSLLELR
                     CQDRTIWFVDKFDSGKHKTHEIILSNANVSVPSVFPKRPIELTEGRLLWLSDLHFDEN
                     KKYHQFDQRDQKNLSAIIKDWAQEVEGVLISGDITWRATENEFKQAEEFIENLCSSKR
                     VNIDGIGMCPGNHDVSFSEGYSADVKKALVKYHEMQHGNGNLSSDEWELLIAVDVLPE
                     FKRNYEQFFRNIVSTDANQYLSMGKRFLIMNQKIVDVCFLNSNSLQQHKLAFQGQGYV
                     GVKQRDDAAKEMGWKRNKKITGGYRVVVLHHNLYPVNYAETPYIGVASGLVYDTEAIL
                     KWCFENGVDLILHGHTHERCVTKVSRKVDNHDKSVWIVSLGSTGVIQGHLVGCNEFAE
                     LDFKGDRIKVIFYNIKHNTIEHNGEIILD"
     gene            2258..>2681
                     /locus_tag="ACIE68_004832"
     CDS             2258..>2681
                     /locus_tag="ACIE68_004832"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFX5910982.1"
                     /translation="MKKLLITDLDNTLYDWVSFYAQSFSAMFDKLVEILEVSRDELTN
                     DFKKVHLKHGNSEYPFATLELESVKRKFNGVSKEIVLSELDEAFHAFNSVRKRTLVCY
                     PGVLETLEELKSRGVVIVGHTEAPVRNALFRLESLGLKK"
BASE COUNT          836 a          466 c          604 g          775 t
ORIGIN      
        1 gtcgttatat tcgttcgcat gaagccgttc agcacatcag catccgcgac cggctgaatg
       61 attttatgca gcagcacggc acagcactgg cggcggcact ggcaccggag ctgatgggat
      121 acagtgagct gacggccatt gcccgaaact gtgccataca gcgtgccaca gatgccctgc
      181 gtgaagccct tctgtcctgg cttgcgaagg gggaaaaaat taattattcc gcacaggata
      241 gtgacatttt aacgaccatc ggattcaggc ctgacgcggc ttcggtggat gacagccgtg
      301 aaaaattcac ccctgcgcag aacatgattt tttcgcgtaa aagtgcgcaa ctggcatcac
      361 atcagtctgt gtaaaattcc ccgaaaatcc gcccgtttta ctgaaaaaag ccatgcatcg
      421 ataaggtgca tggctttgca tgcgttttcc tgcctcattt tctgcagacc gcgccattcc
      481 cggcgcggcc tgagcgtgtc agtgcaactg cattgaaacc gccccgcaaa gcgggcgggc
      541 gaggcgggga aagcactgcg cgcttcccta ccaatgattt taattgtaat ttatatcagt
      601 agagagaacc cgacatttaa atgaatattg aaggaatata aatgaattgc aaaactatca
      661 ttcttcgttt cagggatttg gtcacacccg ccggagaaac aatcactcta catcaggata
      721 taatcaaatc taagggctca gtttggtggg gatggtgggc aaaagctgat gaacaatgtc
      781 caagggagtt taatgattta aaagatcaaa tatctgaaaa taatcctttg gaaatatacc
      841 tttttgatag tggacagttg aaaatatact ttgcaaattt aatcggtatt tctaccaatt
      901 tcgataagca cccttgtcct gttcatgaca tgacaccgcc atattatagt gatcagcaat
      961 ataatgtgtg gttcaatttt tcatctatag aagaagtttc tgattgttca ggtttaataa
     1021 atgggctagc atactcaggt gctgtgaaag attttttcaa aaataatgat atgttccaaa
     1081 tttactcggg taagcaaata tcttccttac ttgagttgcg ttgtcaagat aggactatat
     1141 ggtttgttga taaatttgac tcaggcaagc ataaaactca cgaaataata ttgagtaatg
     1201 caaatgttag tgttccctct gtctttccaa aaaggccaat cgagttaact gaaggaagac
     1261 tgctttggtt atctgattta cactttgatg aaaataaaaa atatcatcaa ttcgatcagc
     1321 gcgatcaaaa aaatctaagt gcaataataa aggattgggc acaagaagtt gagggggttc
     1381 ttatctccgg ggatataact tggcgcgcaa cagaaaatga gttcaagcaa gcagaagagt
     1441 ttatagaaaa cttatgctca tctaaaagag taaatattga tggtataggt atgtgccctg
     1501 gtaatcacga tgttagtttt agcgaaggtt actcggcgga tgttaaaaag gctctagtga
     1561 aataccacga aatgcagcat ggtaatggga atctgtcaag cgatgagtgg gaattattaa
     1621 ttgcagtaga tgtgttgcca gaatttaaaa gaaactatga acagttcttc agaaatattg
     1681 ttagtaccga cgccaatcaa tatttgtcaa tgggaaaacg ttttttaata atgaatcaaa
     1741 aaatagtgga tgtatgcttc ttgaactcaa actcattaca gcaacataaa cttgctttcc
     1801 agggacaggg ctatgtaggt gtcaaacaaa gagatgatgc agcaaaagaa atggggtgga
     1861 aacgtaataa aaaaatcaca ggtggttatc gagttgtagt tttgcatcat aatttgtatc
     1921 cagttaatta cgctgaaact ccttatattg gggttgctag cggtttggta tatgatacag
     1981 aagcaatttt aaaatggtgt tttgaaaatg gtgttgatct cattcttcat gggcataccc
     2041 acgaacggtg tgttactaag gttagtagaa aggttgacaa tcatgataaa tcagtatgga
     2101 tcgtcagttt aggtagtaca ggcgtcattc aaggacatct tgtcggatgc aacgaattcg
     2161 ctgagttaga tttcaaaggc gacaggataa aggttatatt ttataatatt aaacataata
     2221 ccattgaaca taatggtgaa attatactgg actaattatg aaaaaactgt tgataactga
     2281 tcttgataac acactttacg attgggtctc attttacgct caatcttttt ctgctatgtt
     2341 tgataaattg gttgaaatat tagaagtatc cagagacgaa cttacaaatg attttaaaaa
     2401 ggtgcactta aagcatggga attctgagta tccatttgcg actttggaat tagaatcggt
     2461 caagcggaag ttcaatggag tttcaaagga gattgttttg tctgagttgg atgaagcatt
     2521 tcatgctttc aactcggtta gaaagagaac attagtttgt tatcctggag tgttagaaac
     2581 cttagaagag ctcaagtcac ggggtgttgt cattgttgga catacagaag ctccggtcag
     2641 gaatgctcta tttcgtctag agtcgttagg cttgaaaaaa t
//
LOCUS       DBANUU010000302         2679 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000302 DBANUU010000000
VERSION     DBANUU010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.308"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(38..1540)
                     /locus_tag="ACIE68_004833"
     CDS             complement(38..1540)
                     /locus_tag="ACIE68_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFX5910983.1"
                     /translation="MNIRKLFCPGNTPRILLFLFFFVVSAITTIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTTDEAAEF
                     TGMIPVYHFLVSAAILVFMVIFWRTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVG
                     TTDTLREMQRYKQLNQHGADNWKILPGVPLYDTIVIVTGESVRRDYMSVYGYPVPTTP
                     WLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFL
                     HMMGSHPNPCDRLHSWPNHYLEQYPRKVACYLASISKLDNFLGQLDGILRRHSRHFVM
                     LYFSDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSVSRKISARHFAGIFQ
                     WLTGIRTENIPPFNLLTDEDNEPIMVFNGERNVPADSLKPQPLILPVKGK"
     gene            complement(1725..2471)
                     /locus_tag="ACIE68_004834"
     CDS             complement(1725..2471)
                     /locus_tag="ACIE68_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFX5910984.1"
                     /translation="MKKVFAVSVVGAACVFAANAGAEDGKSGFYLTGKAGASVVSLSD
                     QRFLSGDEEETSKYKGGDDHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKY
                     NIDKGKGSWSDGYWRDDLKNEVSLNTLMLNAYYDFRNDSAFTPWVSAGIGYARIHQKT
                     TGISTWDYGYGYSGRESLSRSGSADNFAWSLGAGIRYDVTPDIALDLSYRYLDAGDSS
                     VSYKDEWGDKYKSEVDVKSHDILLGVTYNF"
BASE COUNT          763 a          661 c          580 g          675 t
ORIGIN      
        1 aaactcatcg tacataatct ggtgatgaac attacgctta ttttcccttg accggaagaa
       61 tcagaggctg cggtttcaga ctgtctgccg gtacattcct ctctccgtta aaaaccataa
      121 tgggttcatt atcttcgtct gtcagtagat tgaatggcgg tatattttca gtacgaatgc
      181 cggtcagcca ctgaaaaata cctgcgaaat gacgggcact gatttttctg ctgacggact
      241 gatgagacgt gatgtcactg gcggtaataa tcaggggaac gctgtagcct ccctgcacat
      301 gaccatcatg atgaacagga ttagcactgt cgctgaccga cagcccatgg tcagaaaagt
      361 aaagcatgac gaaatgacgg gaatgccggc gaaggatacc atcaagctga ccgagaaagt
      421 tatccagttt actgatgctg gcgaggtaac aggcaacctt tcggggatac tgctccaggt
      481 aatgattcgg ccaggagtga agccggtcac acgggttcgg atgagacccc atcatgtgca
      541 ggaatatcac cttcggagag gatttatccg ccagtgcacg ttctgtttcc tgtaacaaca
      601 acatgtcatc cgttttacgg gaagcaaagc tgcctttctt gaggaaaacg gtatgctccg
      661 catcagaagc aataacagag atgcgtgtat catgctcccc cagctttccc tgattggata
      721 tccaccatgt gctgtatcct gcttttgctg ccagcgccac cacgttgttg ccggaatcag
      781 ggttctgctc atagtcataa atcagtgtcc ggctcaggga aggcacagta ctggctgctg
      841 ccgatgtata accgtcaata aataaaccgg gagccgtatt cagccacggt gtggttggca
      901 caggatagcc atataccgac atataatccc tgcgcacact ctcaccagtg acgataacaa
      961 tcgtgtcata caacggtaca cccggcagga ttttccagtt gtcagccccg tgctgattca
     1021 gttgtttata acgctgcatt tcacgcaatg tgtcagttgt ccccacaaca gttcctttaa
     1081 ccatccgcaa cggccagctg tttactgagc ataatacgaa cagcagcagt gccagccagt
     1141 tacggtgacc gcggtggtgt gttcgccaga aaatcaccat gaataccaga atcgcggcac
     1201 tgaccagaaa atgataaaca ggaatcatcc cggtaaactc cgctgcctca tcagttgtgg
     1261 tctgcaacaa cgcgacaata aaactgttgt tgattttacc gtacgtcata ccggcaggcg
     1321 catacagtgc acaacagaac agaaataaca gcgctgtaat ggatgtgagg gtatttctgt
     1381 gtgcaaggag cagaaggaga aacagaagca gcacatttcc tgttgcattc ttctcagtgt
     1441 atccgcatgc aattgtggtt attgcagaaa caacaaaaaa gaataaaaac aataaaatcc
     1501 ggggggtgtt gcccggacaa aacagttttc tgatattcat cggagtatat cgacaacatt
     1561 attatgaaga gaacaagata ataaaaatca gaaattatct gtgaaacaga taacagacaa
     1621 ccctgcagta taatattact gcagggtgtt cctttttaat tacagaaata cgtaattatc
     1681 ttaattgcag aaatatgcgc aattatcgtt cagaagcagt gtcgtcagaa gttataagtc
     1741 acaccaagca ggatgtcatg acttttaaca tcaacctctg atttatattt atcgccccac
     1801 tcgtccttgt aactcacact gctgtcaccc gcatcaagat agcgatagct gaggtccaga
     1861 gcgatatccg gggtgacgtc atagcggata cccgcgccaa ggctccatgc gaagttgtca
     1921 gcagagcctg aacgtgacaa cgattcacga ccactgtatc cgtacccgta atcccaggtg
     1981 ctgataccgg ttgttttctg atgaatcctg gcgtaaccaa tccctgctga tacccatggt
     2041 gtgaatgcac tgtcattccg gaagtcatag tatgcattca gcatcagagt gttgagtgat
     2101 acctcattct taaggtcatc acgccagtaa ccatctgacc agctgccttt acctttatct
     2161 atgttatact tcgaatcagc ttttccacgt gcgtaaaact ccagttctgt acgaaccgga
     2221 atactgaact gcggataaaa atcataaccg gctgcgatac cgccactgaa taccgtgtca
     2281 tggtcatccc cacctttata ctttgacgtt tcttcttcat ctcctgacag gaaacgctgg
     2341 tctgaaagtg acaccacaga ggcaccggct ttaccggtca gataaaaacc gcttttaccg
     2401 tcctcagcac ctgcatttgc tgcaaataca caggctgcgc ccaccactga aacagcaaaa
     2461 acctttttca tttcaattat ctccgttatt gtatcatcac tttaatatat atttttaata
     2521 tttaaaaacc ggactaaagg cagcctcccc cctccacact acggaagcca gacttttact
     2581 ctacacaaaa taaatatatt tcctcagtga ggacgtgaac gccagcctga aattatgtgt
     2641 atacaacctc accagaatac gaacaggcag gggcactgg
//
LOCUS       DBANUU010000303         2667 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000303 DBANUU010000000
VERSION     DBANUU010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.309"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(185..403)
                     /gene="ymgF"
                     /locus_tag="ACIE68_004835"
     CDS             complement(185..403)
                     /gene="ymgF"
                     /locus_tag="ACIE68_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFX5910985.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
     gene            complement(535..2058)
                     /locus_tag="ACIE68_004836"
     CDS             complement(535..2058)
                     /locus_tag="ACIE68_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFX5910986.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNSPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAIINGEIVPFYQPVVNGLEGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(2390..2638)
                     /gene="ymgC"
                     /locus_tag="ACIE68_004837"
     CDS             complement(2390..2638)
                     /gene="ymgC"
                     /locus_tag="ACIE68_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFX5910987.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT          821 a          580 c          546 g          720 t
ORIGIN      
        1 aataactctt cgacatcaca atcattgata gttgccatag aaattatttc aatgatatat
       61 aaaaacaact gtgataacac ctgggttgac aaatatttgc gcaataattc ctttccttca
      121 atgatccagg cgttcactgt cagcacccca ttcagccgca tactgacagt gagaaactga
      181 aagattattg ctgatccctg tggctgacga gtctttccct taatcgatta accagattga
      241 tcgccagcca aacaagggca ttaagcaaat ttatcgtcac aagcacttct cccagcccgg
      301 tactgggttt tgcaataagg atgaccagga gagtgatcag cataaatgca atggaaaaga
      361 tgatatagag agtgaaataa tccagattat tactattgtt catgctacta atggcaatcc
      421 tgtcaaaact ttcaaacaat tatagcaaac aaaataatga acaatagtaa caagaggcaa
      481 cgagctaatt agctgtctat aattacaccg caccatggcc ttattgatca attttcactc
      541 aaccacaacc ttcaccttcg gtttagaaag gataattttg accaggtcga tgtatgtaac
      601 aggtttatag aaataataac cctgctgaaa tgtgatatta ttttggttca gatagtcaag
      661 ttgttctttc gtttcgacac cttcagcgac gatactgatc gaaagtttac gcgccagatc
      721 caatacacaa tccagaattc gggttgattc tgggtctgcg tttacgcggc caacgaaact
      781 gtgatcaatt ttgatataat caatatgcag gtcatgcaga taagaaagcc ctgagtagcc
      841 agtaccgaaa tcatccagcg cgatgacaaa accattttca tgcagaatgt tcaaccgctg
      901 aaccagactt tcatcaacat ttaatggctc acgctcggtg acttccagaa cgaggtttaa
      961 atcgcggcgg gtaaaactat cacggtaatt taagcactcg tcgacaaacg tcggcgaaat
     1021 aatatgcgag gcgctaaaat taattccaat atgaaaacct tccggcagtt tactcgcgat
     1081 agcgttcatc tgtctggcaa cctgattcat caggctttgc gtaagcggaa cgattaatcc
     1141 ggatttttca gcaagtggaa taaatgccgc gggtgatata tatccaccgt ggggttgttt
     1201 ccagcgagct aacacctcaa ctccccgcaa tgttccttcc agaccattta ccacaggttg
     1261 gtaaaaaggg acgatctccc cattaattat agcccgtcgc aggatctctt caggcgttgc
     1321 gcttttattg aaatacctat caagcaggaa ggcagcggcg caggcgatta acagaataaa
     1381 gatcaaaatc ccaaagcctt tatcgataag tctgtagaag ctaaagagtg gcggcgagtt
     1441 aaagataata gtgaaatgat attttgtgga gtgaaccttc tcggtatagg gataatgtcc
     1501 attaagcgtc atcacatcac ccgtaggtcc aataatgtca tccgccacgc gtaatacata
     1561 ccttacccct ttcaagggta cattaagcgc cccgcgaata tgctgatcac ttatcgtaac
     1621 caaaatgcgc gtatctgcaa attggctctg atagagcagg ataggtaatc tattaacggt
     1681 gtcgattgcc ggagccaaca gtaaattact gtccgggaaa acaggaatac gagacaacag
     1741 gacccgattc ccaggcaggg atgtacacca gactattccc tgtttgagaa tgatgattgt
     1801 gcgcaggtga ggtttcagag ctgcttcagt gccaagctga tattgctcct cgaggtcgca
     1861 ttccttcccg gcaatgttaa ccgctgtttg agtcgcctgt gacgcttctt caagtatatg
     1921 attgatattg ttggcggcat atctcgctcc cgccaggtac tctgcttttg cggaatacca
     1981 gagctggatg tttaaaatag cgacaccggt tataaacaag catatcgcaa cgaggatggg
     2041 tatgagtgta ttgcgcattt gcttgtcccg gccaaagggt tttatacatg aaaatgccat
     2101 tcattcgctc gcgctggcat ctacagagag catggtagag agcataggaa atatccacgc
     2161 cgaagataaa attttctcca tcttgatcta acccattaat tattgagcat aatgtaggca
     2221 ggattaaata aattaggtgg gatatcaggc atcaagaatg gagaacgaga actctccatt
     2281 cttgacaact gatattgcgg atatattaag aaagcataac gcctgaaatg ctcactttgc
     2341 accagcatga tgatacagct gatgtttatt ctataacctt actcaagttc taagagagca
     2401 cggattccct gtcattaata atgatatccg ttagtgtcgt cttatgcatt tcaatgtact
     2461 gcaagatttg cagatagaga tcagaaagaa tcacactggt ttcatctttt aaacgcaagc
     2521 gtaaagcctg atcaataata tgactatcaa catacccatg cgtcatgaac acctccctct
     2581 cgagattttt aaacaaagca cattgaaaaa taaatctctc tgggattgaa ttgttcatgt
     2641 cagcctcgta gaaatattaa cccaata
//
LOCUS       DBANUU010000304         2652 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000304 DBANUU010000000
VERSION     DBANUU010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.310"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(233..1204)
                     /locus_tag="ACIE68_004838"
     CDS             complement(233..1204)
                     /locus_tag="ACIE68_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HFX5910988.1"
                     /translation="MKRAPVIPKHTIKTQPLEDTPSSAPAAPMVDSLIARVGAMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQRAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRIPTECIERIEAILKELEKAAL"
     gene            complement(1204..2370)
                     /gene="sopA"
                     /locus_tag="ACIE68_004839"
     CDS             complement(1204..2370)
                     /gene="sopA"
                     /locus_tag="ACIE68_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HFX5910989.1"
                     /translation="MRLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
BASE COUNT          748 a          655 c          558 g          691 t
ORIGIN      
        1 ccgtggtccc agaccgataa tcagaccgac aatatgagtg ggaccgtggt cccagactga
       61 ttatcagacc gacaatatga gtgggaccgt ggtcccagac tgattatcag accgacaata
      121 caactgggac cgtggtccca gatataatat tcaggccagt tagttatgtt ttctagcctg
      181 tgctaaagga aattaagtaa agacagataa atgtagacta aaacgtgccg catcaaagtg
      241 ccgctttttc aagttcctta agaatggctt caattctctc tatacactca gttggaatac
      301 gagatctatc taggtttaac accattttat cgcccttgta caacactgtc gctccaggag
      361 caaactgatg tcgtgagctt aagctagttc ttgaggcaga tgacgtttta agcacagacg
      421 ttaaaagagt gatgacttct tcagcttcaa atatcactcc tgcttttttc tgctcgtgaa
      481 ggttagatgc ctgctgctta agtaattctt ctttatctgt aaaagctctt tgaagtgcat
      541 cacctgaccg ggcagatagt tcaccgggat gagaaaaaag agcaacaact gatttaggca
      601 gtttagctgt gttgatacag cgggtaataa tcttacgtga aatattttct gcatcagcca
      661 gcgcagaaat atttccagca aattcattct gcaatcggct tgcataacgc tgaccacgtt
      721 cataagcact tgttggccta taatcgttac ccaatctgga caatgcagcc atctgctcat
      781 catccagctc accaaccaga actcgataat cactttctgt aagtgcagca gctttacgac
      841 ggcggctccc atcggcaatt tctatgaccc cagacactct tcgaccgaat gctggtgtct
      901 gctgacctgt cagtagaaaa gaagggataa gatcatccag ggcgtcttcg gtaagcagtt
      961 cctggtcacg ttcattacct gaccataccc gagaggtctt ctcaacacta tctccccgga
     1021 gcacctcaag ggtaaatttc acgtccctac cacatacagg caaagtaata gcattgccac
     1081 gagccatagc tcctacgcgc gcaattaacg aatccaccat cggtgcagct ggtgccgatg
     1141 acggagtatc ttcaagcggt tgagtcttaa tcgtatgttt tggaatgaca ggcgcacgct
     1201 tcattatcta atctcccagc gtggtttaat caaacgatcg aaaatttcat tgcagacagg
     1261 ttcccaaata gaaagggcat ttctccaggc acctgttgaa gagcgttgat caatagcctg
     1321 ttcaaaaaca gttctcatcc ggatctgacc tttaccaact tcatccgttt cacgcacaac
     1381 attttttagg accatgcttc cccaggcgtc ccgaatttgc tcctccatcc acggggactg
     1441 agaaccatta ctattactgt atttggtaag caaaatacgt acatcaggct cgaatccttt
     1501 aagatctacg tttttgagca gatcacgaag catatcgaaa aactgcagag cggaagtgta
     1561 gtcgaacaac tcagcaggcg tggggacaat caacacatca gcagcacata caacattaat
     1621 cgtgccgata cctaggttag gcgcgctgtc aatgacaatg acatcatagt catgagcgac
     1681 ggtttcaatg gccagtcgga gcatcaggtg tggatcggtg ggcaatttac cttcatcaaa
     1741 tttgcccatt agctcagttt caatgcggtg cagagccaaa caggaaggaa taatgtcaag
     1801 cccaggccag caagtaggct ttattgcata agtgacatcg tccttttccc caagatagaa
     1861 gggaaggaga gtatcctctg catgaatatg aagatctggc acccagccgt gatacatcga
     1921 tgctgttccc tgggggtcgt taccttccac gagcagaaca cgtaatccct tcagagccag
     1981 atcctgagca agatgaacag aaacagaggt tttgtaaacg ccccctttat gagcagcaac
     2041 tccaatcacc ggcggaaata cgtcttcagc acgtcgtagt ctcgtaccaa acacgtcacg
     2101 catatgatta atttgttcaa ttgtataacc aacacgttgc tcaacccgtc ctcgtatttc
     2161 catatccggg tgcggtagcc ggcctgcttt ctcggcatcc ctgatagcct gagaagatac
     2221 tccaactaaa tccgctgctt cacctattct ccagcgtcgg gtgattttcc tcgcttccgg
     2281 gctgtcatca ttaaactgtg caatggcgat agccttcgtc atttcatgac cagcgtttat
     2341 gcactggtta agtgtttcca tgagtctcat tctgaacatc ctttaatcat tgctttgcat
     2401 attttcattt aatcttgcat tttattgcaa agcaacaaca aaattgcaaa gtagtcaaaa
     2461 aaccgcaaag ttgtttaaaa tgagagcaac acatcaaaaa aggaaacagg aagagcacat
     2521 acctcagtca ataattatca ctagtactcg tagccgtcgt gtaaccgagc gtagcgagct
     2581 aactggcgag gacgcgcgct aactgttcac actcagtatc cctagcacca aaataactat
     2641 ctgatggtca ag
//
LOCUS       DBANUU010000305         2644 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000305 DBANUU010000000
VERSION     DBANUU010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.311"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(152..1332)
                     /locus_tag="ACIE68_004840"
                     /pseudo
     CDS             complement(152..1332)
                     /locus_tag="ACIE68_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            1919..2148
                     /locus_tag="ACIE68_004841"
                     /pseudo
     CDS             1919..2148
                     /locus_tag="ACIE68_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          647 a          652 c          666 g          679 t
ORIGIN      
        1 taaaaaatcc tgtactcaca gggtgatgcg ggataactga cgtaaggagt gctgcaggga
       61 tttgatggtc ccttgtgctg atatgaatac ggatatttta tacagaatac ttttcagtac
      121 aagcgactgt attacagtct gccatatatg attacctgtg atcaggaaga ataagtggct
      181 gaggtttcaa actgtctgcc ggtatattcc tctctccctt aaaaaccata acaggttagt
      241 tatcttcgtc tgtcagcgga ttgaatggcg gtatattttc agtacaaata ccggtcatcc
      301 actgaaaaat acctgcgaaa tgacgggcac tgatttttct actgacgggc tgatgagacg
      361 ttatgtcact ggcggtaata atcagtggca cactgtagcc tccctgcaca tgaccataat
      421 gatgaacagg attggcactg tcgctgaccg acagcccaag gccagaaaag taaagcatgg
      481 cgaaatgacg tgagtaccgg cgaaggatac catcaagctg gccaagaaag ttatccagtt
      541 tactgatgct ggcgaggtaa caggcaattt ttcggggata ctgctccagg taattattcg
      601 gctaggagtt aaggctgtca cacggatttg gatgagaacc catcatgtgc aggaaaatta
      661 tcttcggaga ggatgtatcc gccagcgcac gttctgtttc ctgtaacaac agtttgtcat
      721 ctgttttacg ggaagcgaag ctgcctttct tgaggaaagt ggcatgctcc gcatcagaag
      781 caataacaga gatgcgtgtg tcatgctccc ccagttttcc ttgattggat atccaccatg
      841 tgctgtatcc tgcttttgct gccagcgcca ccacattgtt gccggaatca gggttctgct
      901 catagtcata aatcagtgtc cggctcaggg aagacactgt actggaggct gtcgaggtat
      961 aatcgtcaat aaataaaccg ggtgccgtat tcagccacgg tgtggttggt acgggatagt
     1021 catacactga catataatcc ctgcgcacac tctcaccagt gacaataaca atcgtatcat
     1081 ataacggtgc tcccggctgg attttccagt tgtcagcccc gtgctactca gttgttcata
     1141 acgctgcatt tcacgtaatg tgtcagttgt ccccacaact gttcctttaa ccatccgcaa
     1201 tggccaacgg tttacagagc ataatacgaa tagccgtaga gccagccagt tacggtggtc
     1261 gcgatggtgt gtccgccaga aaatcaccac gaataccaga atcgcggcac tgaccagaaa
     1321 atgataaaca ggaaacacgg ggcggttcac tttgctttct cctgtagtgt tttctgtagg
     1381 ttctgaaccg gttctagcgg ggttgtgagg ctgatggcaa tcgccgggct cttcctgcat
     1441 gttacactgg tactgcaaga gggggccttc gctcctctga cattactcag tattttcact
     1501 actacacccc atctgccatc cataacaacc gattcacccc ctcgcgaggc gttgttgccc
     1561 gtcacccgga cagacaccgt tatggacttc tcttgttgca cgatcgatca attgttatgc
     1621 atgctgacac cacaaccccg gtgggtatga ccgatgcctt tctcactctc ttcccggtca
     1681 tcgtcggcct tccccgttat tgtggcgtgt cagcaccctc gacggcattt cgatggctgc
     1741 tcagtgttca ctcacgttac ggcccgcata acccgttgac ttcctgaaga agtcgtttca
     1801 gggagagatt cagccctttt gttacctcct ggaccgcttc ttgtccttcc agtcggagcg
     1861 agagttagcc ggggcggact ttcaccgacg gaaagatcgt gcatttcaag gcacacacaa
     1921 caatatctgc gagcgtgcta tccgtccggt cgttatggga cgaaaggcct ggttgttcgc
     1981 tggttcactg gtggccggga accgcgcggc acagataatg agccttctgg gaaccgcagt
     2041 ctggagccac atgcttggct gacggacgtc ctgacgcgtc tgccggagtg gccggaggag
     2101 aggttggctg agttactgcc tcttgagggc tttaccttct tcgggtaagt gatacctgcc
     2161 gtcaggtgtt tgtgcaccgg gccataacct gcagttggga attgaacgcc tgacaacagg
     2221 aaatgagcta gagcagtaaa cacgcgggct gacaatgggt cagaatatga gttagcccgg
     2281 cgttattcct ggggtcagta cttcagatct agcctccggt accagactag ctagccccta
     2341 cgcaaccaaa taaatcaacc atcgtcagag tgcaatcgtt catatacact gtcggtgatg
     2401 cgctaaaaaa tgcgttaatg ttgagttcct cgaaccctta ccaattaagg gacatagcca
     2461 gatacgcaac tggctgatgc aagcagtcta atgtctttag atccgctggc ctgcgggcat
     2521 gacaatgtca aacagtcaaa gattgtggcg ttgacggtca gtcttgggga caaaaaagct
     2581 cagtattctc gttggcaacc agataattta acagttgatg ctgaaacgaa ataggcatct
     2641 tggt
//
LOCUS       DBANUU010000306         2631 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000306 DBANUU010000000
VERSION     DBANUU010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.312"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..168
                     /locus_tag="ACIE68_004842"
     CDS             <1..168
                     /locus_tag="ACIE68_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFX5910990.1"
                     /translation="EFVFVFAAVRRADRHPRICMLRTVAGDERSARRSLVRDYVLSLA
                     ARLPVMEVSRA"
     gene            161..448
                     /locus_tag="ACIE68_004843"
     CDS             161..448
                     /locus_tag="ACIE68_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HFX5910991.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            441..896
                     /locus_tag="ACIE68_004844"
     CDS             441..896
                     /locus_tag="ACIE68_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5910992.1"
                     /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPDIVWNP
                     YRRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYLDIYPQDTVPVSRAAPVAREWREIPRTEPDPETTPDNSYRNYL"
     gene            1032..1352
                     /locus_tag="ACIE68_004845"
     CDS             1032..1352
                     /locus_tag="ACIE68_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HFX5910993.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            1367..>2631
                     /locus_tag="ACIE68_004846"
     CDS             1367..>2631
                     /locus_tag="ACIE68_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFX5910994.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNHHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDAAAKVAAVTGSLPPADPAVTAAA
                     VAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRRLIGFRNGVL"
BASE COUNT          538 a          785 c          766 g          542 t
ORIGIN      
        1 gaatttgtct ttgtgtttgc ggctgtccgt cgcgcagacc gtcatccccg tatctgcatg
       61 cttcgcaccg tcgccggtga tgaacgcagt gcccgccgtt cccttgtccg tgactatgtg
      121 ctctcccttg ctgcccgtct gccggtgatg gaggtgtccc gtgcgtaata aaaaagcccc
      181 tcagaccgtc tcagcgcgtc atgacgcccg tgaacacctc agcattgagg cttaccataa
      241 gctcaaccgc gccagcgccg tatcccagtt tgttgggggt gatttgattc accgtgaact
      301 ctccggcctg catcagctct acattccgca tattttcagc tacctgaatg aagatattga
      361 ttttgtgctg aatgagctga aagccaaagg cctgtgccgc gattttctcg cccagcagaa
      421 agaccgggga gacaggacgc atgtttgatt ttccccagcc cggtgagatt taccgttctg
      481 ccggttttcc cgatgtggcc gtggtcggca ttctggaaga cggtattccg tgggaaatgc
      541 cgtaccgctg cccggacatt gtctggaatc cgtaccgccg taaattcagt atccttgtgc
      601 gtatcctcgc cgacgggcgc accacagaca tcccgctggg gcgttttctg cgggaattta
      661 cctgtgaccg tcctgacctg tttaaacgca gccccgtaaa ccggcatgcg gtactgaaag
      721 aaatggccgg agacccggaa ttacagaaat ggcgggagaa atatctggat atttacccgc
      781 aggacactgt tccggtcagc cgggcagcac cggtagcgcg ggaatggcgg gaaattcccc
      841 gcacggagcc tgacccggaa accaccccgg ataacagtta ccgcaattat ctgtaattaa
      901 aaaacgacac ccgaaaaatt aaatgtgcgt attcgcgcag ggatacgcac gtcttcagga
      961 gacgcagata tgccttatca gttaatgcaa ccggcacgga atgcagtcat ctgtcacagg
     1021 gaggaaaaca aatgaaaaca cccttaccgc ccgtcttacg cgctgccctt taccgtcgcg
     1081 ctgtcgcctg tgcctggctg accgtgtgcg aacgtcagca ccgctacccg catctcaccc
     1141 ttgagtcact ggaggcggcc atcgccgctg agctggaagg cttttatctg cgccagcacg
     1201 gtgaggaaaa agggcgtcag atagcctgtg ccctgctgga agatttaatg gaatccggcc
     1261 ccctgaaggc cgcgccgtcg ctgtcctttc tcgggctggt tgtgatggat gaactctgtg
     1321 cccgtcacat aaaagcgccg gtactgcact gaaggagaac aacaccatga aaatgaacgt
     1381 aaccgccacc gtcagccatg cgctcggcca ctggccgcgt attctcccgg cgctggggat
     1441 tcaggtgctg aagaaccatc atcagccctg tccggtctgt ggcgggagtg accgcttccg
     1501 ttttgatgac agggaggggc gcggcacctg gtactgcaat cagtgtggtg ccggtgacgg
     1561 cctgaaactg gttgaaaagg tgtttggtgt ctccccgtcc gacgcggccg caaaggtggc
     1621 tgccgtgacc ggcagtctgc ccccggctga cccggcagtg acggccgccg ccgttgctga
     1681 aacagacgct gcccggaaga acgccgccgc actggcacaa accctgatgg caaaaacccg
     1741 tcccggaacc ggtaacgcct acctgacccg caagggcttt cccggccggg aatgccggat
     1801 gctgaccggc acacacagag ccggtggcgt gagctggcgc gccggtgacc ttgtggtgcc
     1861 actgtatgac gacagcggcg aactggttaa ccttcagtta atcagtgctg acggccgtaa
     1921 gcgcaccctg aaaggcggac aggtcagggg cacctgtcac acccttgaag gacagaatca
     1981 ggccggaaaa cgtctgtgga tagcggaggg atacgcgacc gcacttaccg tacatcacct
     2041 gaccggtgaa acggtgatgg tggcgctttc ttccgtgaac ctcctttctc tggccagcct
     2101 tgcccggcag aagcatccgg cctgtcagat tgtccttgcc gcagaccgtg acctcagcgg
     2161 tgacggccag aaaaaagccg ccgcagccgc agatgcgtgt gaaggtgttg ttgccctgcc
     2221 gccggtcttc ggtgactgga atgatgcctt cacgcagtac ggcggggaag ccacccgtaa
     2281 ggccatttat gatgccatcc ggccaccggc tgaaagcccg ttcgacacca tgagcgaagc
     2341 ggagttttcc gccatgagta ccagcgaaaa ggccatgcgt atctatgagc attacggcga
     2401 ggcgctcgcg gtcgatgcca acggccagct tctgtcccgc tatgaaaatg gtgtctggaa
     2461 ggtgctgccg ccacaggact ttgcccggga tgtggccggg ctgtttcagc gtctgcgcgc
     2521 gccgttctcc tccgggaagg tggcctccgt ggtggacacc ctgaagctga ttattccgca
     2581 gcaggaagcc ccctcccgcc gcctgattgg ctttcgtaac ggcgtgctcg a
//
LOCUS       DBANUU010000307         2615 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000307 DBANUU010000000
VERSION     DBANUU010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.313"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            222..1388
                     /gene="nhaA"
                     /locus_tag="ACIE68_004847"
     CDS             222..1388
                     /gene="nhaA"
                     /locus_tag="ACIE68_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFX5910995.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1454..2353
                     /gene="nhaR"
                     /locus_tag="ACIE68_004848"
     CDS             1454..2353
                     /gene="nhaR"
                     /locus_tag="ACIE68_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFX5910996.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2391..>2615)
                     /locus_tag="ACIE68_004849"
     CDS             complement(2391..>2615)
                     /locus_tag="ACIE68_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFX5910997.1"
                     /translation="NELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQ
                     IPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          602 a          582 c          677 g          754 t
ORIGIN      
        1 attaatccct caggaatcct caccttaagc tatgattatc taggcttagg gtcactcgtg
       61 agcgcttaca gccgtcaaaa acgcatctca ccgctgatgg cgcaaattct tcaatagctc
      121 gtaaaaaacg aattattcct acactataat ctggttttaa cgatgattcg tgcggggtaa
      181 aatagtaaaa acgatctatt cacctgaaag agaaataaaa agtgaaacat ctgcatcgat
      241 tctttagcag tgatgcctcg ggaggcatta ttcttatcat tgccgctatc ctggcgatga
      301 ttatggccaa cagcggcgca accagtggat ggtatcacga ctttctggag acgccggttc
      361 agctccgggt tggttcactc gaaatcaaca aaaacatgct gttatggata aatgacgcgc
      421 tgatggcggt atttttcctg ttagtcggtc tggaagttaa acgtgaactg atgcaaggat
      481 cgctagccag cttacgccag gccgcatttc cagttatcgc cgctattggt gggatgattg
      541 tgccggcatt actctatctg gcttttaact atgccgatcc gattacccgc gaagggtggg
      601 cgatcccggc ggctactgac attgcttttg cacttggtgt actggcgctg ttgggaagtc
      661 gtgttccgtt agcgctgaag atctttttga tggctctggc tattatcgac gatcttgggg
      721 ccatcattat catcgcattg ttctacacta atgacttatc gatggcctct cttggcgtcg
      781 cggctgtagc aattgcggta ctcgcggtat tgaatctgtg tggtgtacgc cgcacgggcg
      841 tctatattct tgttggcgtg gtgttgtgga ctgcggtgtt gaaatcgggg gttcacgcaa
      901 ctctggcggg ggtaattgtc ggcttcttta ttcctttgaa agagaagcat gggcgttctc
      961 cagcgaagcg actggagcat gtgttgcacc cgtgggtggc gtatctgatt ttgccgctgt
     1021 ttgcatttgc taatgctggc gtttcactgc aaggcgtcac gctggatggc ttgacctcca
     1081 ttctgccatt ggggatcatc gctggcttgc tgattggcaa accgctgggg attagtctgt
     1141 tctgctggtt ggcgctgcgt ttgaaactgg cgcatctgcc tgagggaacg acttatcagc
     1201 aaattatggt ggtggggatc ctgtgcggta tcggttttac tatgtctatc tttattgcca
     1261 gcctggcctt tggtagcgta gatccagaac tgattaactg ggcgaaactc ggtatcctgg
     1321 tcggttctat ctcttcggcg gtaattggat acagctggtt acgcgttcgt ttgcgtccat
     1381 cagtttgaca ggacggttta ccggggagcc ataaacggct cccttttcat tgttatcagg
     1441 gagagaaatg agcatgtctc atatcaatta caaccacttg tattacttct ggcatgtcta
     1501 taaagaaggt tccgtggttg gcgcagcgga ggcgctttat ttaactccac aaaccattac
     1561 cggacagatt cgagcgctgg aagagcgcct gcaaggcaaa ttatttaaac gcaagggacg
     1621 tggtctcgaa cccagcgagc tgggagaact ggtctatcgc tatgccgata aaatgttcac
     1681 cttaagccag gaaatgctgg atattgtgaa ctatcgcaaa gaatccaatt tattgtttga
     1741 cgttggcgtg gctgatgcac tttccaaacg cctggtcagt agcgtactta acgccgcagt
     1801 ggtagaaggc gagcccattc atcttcgctg cttcgaatcc acccacgaaa tgctgctgga
     1861 gcaattaagt cagcataaac tggatatgat catttctgac tgtccgatag actctacgca
     1921 gcaggaaggc ctgttctccg tgagaattgg cgaatgtggc gtgagtttct ggtgtacaaa
     1981 tccaccacca gaaaaaccgt tcccggcttg tctggaagaa cggcgacttt tgattcctgg
     2041 gcgacgttca atgttagggc gcaaattgct taactggttt aactcccagg gattaaacgt
     2101 agaaatcctc ggcgagtttg atgatgccgc tttgatgaaa gcttttggtg cgatgcacaa
     2161 tgcaatcttc gttgccccaa cgctttatgc atatgacttt tatgccgata aaactgtcgt
     2221 agaaattggt cgcgtcgaga atgtgatgga agagtaccat gctatttttg ctgagcggat
     2281 gattcagcac ccggcggtac agcgaatctg caatacggat tattctgcgc tttttagtcc
     2341 agcggtgcgt taatcggcag ctcccccaaa gttaaggtgg gggagataga ttagttgtac
     2401 attaccacga ttttgactcg gctcattatt tgcccgcttg agacattgtt tccatatgta
     2461 cgcgggcgaa taaatagagg aatctgatta cttccttcat ggggatgctg aaaagagtag
     2521 taattgcttt taccatactg gatttcattc gtaccatcat tggagagttg aaagcctatt
     2581 tgacccgcac cgtcctcact gttttctaac tcatt
//
LOCUS       DBANUU010000308         2582 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000308 DBANUU010000000
VERSION     DBANUU010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.314"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>2582
                     /locus_tag="ACIE68_004850"
     CDS             <1..>2582
                     /locus_tag="ACIE68_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFX5910998.1"
                     /translation="GRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFL
                     GAMTEDDARPEALRRFELMVEEVARNASAVAQNTAAAKKSASDAGTSAREVATHATDA
                     AGSARAASTSAGQAATSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKT
                     SETNAAASQKSAATSASTATTKASEAATSARDAAASKEAAKSSETNASSSASSAASSA
                     TAAGNSAKAAKTSETNADNSAQAAAASQTASANSATAAKKSETNAKNSEAAAKSSETN
                     AKASETNAKSSETNAAKSAADALNYRNQAQSIVGNNIGLGEVAMTCTDISVEPSGYIG
                     FVRIYISAKGYPSVASSAGDNLISGYIVRSEVSTKRYSGLFVGSDTKSLYSYIYSQSS
                     GPQWTRHARKDEVSRFSQNESTTRVYGPTGDRFLEIHTNGNWGVYNSSSGKWSPLGLA
                     QGGTGANSLNDAKTNLQIPEGGLTKAITLQAPEGAEDGKYYPVIIDTSAMEGYGNLAC
                     PIDIKTAGRSASDPMNSNTFSGYFRCGGWSDTRDIACGSFVAYDKNELGILCLKVSQK
                     DYPQYVAFYIHKSAFPILLQTGFKARVTVPKEDYILGTNGVKYKFGVSTSTEGNAENS
                     VKNALDFTGGESGFYSNLPFRQGYAYNVALTNGIVLDNNFVLTAPVFMNNGEVKATKS
                     FTAYGTDVSNRTFVSQRLQSEGGPVVDQTELRAANASGEIVVRDINNSGNSKFFNFNL
                     DGTFSSYQGLVVHTGQNWSTQHTENVNKFKPIAGSAGGPDGTMVVGGFHAQFSGNYVT
                     QFAGRNSKFWARSFEAGVDKGWKRLLTVDDLNSSTDLAVRSLTTSNPVKSGGGRIDVL
                     GSTS"
BASE COUNT          754 a          601 c          645 g          582 t
ORIGIN      
        1 gggcgttaca gcatggacgt tgagtacggt cagtacagcg ttattctgtt ggtggaaggc
       61 ttcccgccat cgcatgccgg gaccatcacc gtgtatgaag actcacaacc gggtacgctg
      121 aatgattttc tcggtgccat gacggaggat gatgcccgtc cggaggcact gcgccgcttt
      181 gaactgatgg tggaagaggt ggcgcgtaac gcgtccgcag tggcacagaa cacggcagcc
      241 gcgaagaagt cagccagcga tgccggcaca tcagcccgtg aggtggcaac ccatgcgact
      301 gatgctgcag gctcagcacg tgcagccagc acatcagccg ggcaggccgc gacgtcggct
      361 cagtcagcgt cttccagcgc aggaacggca tcaacaaagg ctactgaagc atcaaaaagt
      421 gctgccgctg cagagtcctc aaaaagcgct gcagccacca gtgccggtgc cgcgaaaacg
      481 tctgaaacga atgctgcagc gtcacaaaaa tctgcagcca cttctgcatc caccgcgacc
      541 acgaaagcgt cagaagctgc cacctcagcc cgggatgcgg cggcctcaaa agaggcagcg
      601 aaatcatcag aaacgaacgc atcatcaagt gccagtagtg cagcttcctc ggcaacggcg
      661 gcaggaaatt ccgcgaaggc cgcaaaaacg tctgagacga atgcggataa cagcgcacag
      721 gcggcagcgg cctcacaaac tgcatcggca aactccgcga cagcagccaa aaaatcagaa
      781 accaacgcga aaaatagcga ggcagcagca aagagcagcg aaaccaacgc taaagccagc
      841 gaaactaatg ctaaatccag tgaaacaaac gcggcgaaat ctgcggcgga cgcacttaat
      901 tatcgcaacc aggcacaatc aattgttggt aacaatattg ggcttggtga agttgccatg
      961 acctgcactg atatctctgt tgagccatca ggttatattg gatttgtcag gatttacatc
     1021 tcagcgaaag gttatccatc agtagcgtct agtgccgggg ataatcttat ttctggttat
     1081 atagttagat cagaagtgtc aacaaaaaga tattccggtc tatttgttgg ttcagataca
     1141 aaatcattgt attcgtacat ttacagccaa agttctggcc cgcaatggac ccgtcacgca
     1201 agaaaggatg aagtaagccg ctttagtcaa aatgaatcaa caactcgcgt atatggccca
     1261 actggtgatc gtttccttga aatccataca aacgggaatt ggggggttta taattcttct
     1321 tctggaaaat ggtctccgtt aggtttagcc cagggtggca ctggcgcaaa tagccttaat
     1381 gacgccaaaa ctaatttgca aattcctgag ggtggattaa ccaaagctat aaccttgcaa
     1441 gcgccagaag gtgcagagga tggcaaatac taccctgtca taatagacac atccgcaatg
     1501 gaaggttacg gcaacctggc atgtcctatt gatataaaaa cagcagggcg ttcagcatca
     1561 gacccgatga atagtaatac atttagtgga tattttcgat gcggtggctg gagcgacacc
     1621 agagatatag cttgcggctc atttgttgcc tatgacaaaa atgagctagg gatactttgc
     1681 cttaaagtat cgcagaaaga ttacccgcaa tatgtagcgt tctatatcca taaatccgct
     1741 ttcccgatat tattgcaaac aggctttaaa gcgcgagtta ctgtaccaaa agaagattat
     1801 atactcggca caaacggcgt taaatataaa tttggtgtat caacgtcaac agagggtaac
     1861 gccgagaact ctgttaaaaa tgctctcgat ttcactggcg gtgaaagcgg gttttatagc
     1921 aacctgccgt tccggcaagg ctacgcctat aatgtcgcat taacaaacgg gattgttctt
     1981 gataataatt ttgtcttaac cgctccagta tttatgaaca atggcgaggt taaagcaaca
     2041 aaatctttca ccgcgtacgg tacggacgtt tcaaacagaa catttgtttc tcaaagatta
     2101 caatccgaag gaggtccggt tgtcgatcaa actgaactaa gggccgctaa cgcttctggt
     2161 gaaattgttg tcagggacat aaataattca gggaacagta agttctttaa ttttaacctt
     2221 gatggtacct tcagttctta tcagggattg gttgttcaca cagggcaaaa ctggagcact
     2281 caacatacag agaatgtaaa taagtttaag ccaatagcag ggagcgcagg cggtcctgac
     2341 ggaacaatgg ttgttggcgg attccatgct caatttagcg gtaattacgt cacacaattc
     2401 gccggtcgca attccaaatt ttgggcaaga agctttgagg ctggggttga taagggatgg
     2461 aaacgactat taacagtaga cgatctcaat tcatctaccg atcttgctgt caggtcatta
     2521 accacatcta acccggtaaa atctggcgga gggcggattg atgtccttgg aagcacgtca
     2581 ga
//
LOCUS       DBANUU010000309         2567 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000309 DBANUU010000000
VERSION     DBANUU010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.315"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(102..956)
                     /gene="rclR"
                     /locus_tag="ACIE68_004851"
     CDS             complement(102..956)
                     /gene="rclR"
                     /locus_tag="ACIE68_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFX5910999.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRLAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            1182..2507
                     /gene="rclA"
                     /locus_tag="ACIE68_004852"
     CDS             1182..2507
                     /gene="rclA"
                     /locus_tag="ACIE68_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFX5911000.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREERDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT          735 a          574 c          598 g          660 t
ORIGIN      
        1 aaatatataa agcccaaacc accgttggaa atagaaatag ttgaaaggct cacaactccc
       61 ggaatgcgga ttatctttgc cctgatttct ggcggtttta ttcagggtgc aagctgtctg
      121 accctttccc gatattctcc cggagtacaa ccaaactcgc ggacaaacgc cttgtgaaaa
      181 gatgattcac tggcatagcc tactgactca gcgatcacca caacagggag catttcccgg
      241 gaaaacatct gggccgctat ttgtagacgc aactttgtta atacagccag cggcgtggtt
      301 ccggaaacat cacggaaaag ctgggcaaaa cttgcccggg acatgtgggc gatgctggcc
      361 agcgattcga cggtccaggc gtgtcctggc atttccaaca tttgctgtat taccgcacca
      421 agacgtggat gcagaagcaa actgagaatg tttttctcag tattaacctg tgcaatccac
      481 tcacgcaccg cgagggtaaa gaatgtagcg cagatctggc tacacagtgc atccactccc
      541 ggcattgcca atctggattc ctgttgtaaa aacgggatcg cctccctcag ccagttatat
      601 tctacgctgt ggttaaccgg tgctaaaaac agcgtttccg gcaaagacgt taaaaaataa
      661 cgcgccgaat gttgcagccg aagagtgccg cagacaatac aggttgattc attatcgaca
      721 tgactcaggc gatgggctga attttgtggt agcaggacca catttcccgg gcgtaatgta
      781 aaaatctccc ccgtcggcat ttccagcttc gccgctcctt gcgttaacgc atgccaacga
      841 ataaccgata attccccggc accatgtgga agctgccagt cacttcctaa cacgcaattc
      901 ttatcgatcg ttccttgtgg agcgttaagc atcaacaaac ggctaagggc atccatatca
      961 gactcctgag tggtttttga ttacaaacac caacaatctt agtaaataac taatctaata
     1021 ccatcaaata gttaatcatg atttttgttt tgtgattctg attaagaaaa agaaaatacc
     1081 agacgataag acaaaatatc aagcgttgtg aagaaatgtt atttgctctt ttgcgtctat
     1141 agtcatgatg tcaaatgaac gcgtttcgac aggaaatcat catgaataaa tatcaggcag
     1201 tgattattgg ttttggcaag gctggaaaaa cattagccgt cacgctggca aaagcaggtt
     1261 ggcgagtggc tctcatcgaa caatcaaatg caatgtatgg cgggacctgt attaatatcg
     1321 gctgcatccc aaccaaaaca ttggttcatg acgcacagca gcacacagat tttgtccgtg
     1381 ccatacagcg taaaaatgaa gtggttaatt ttttacgtaa taagaatttt cataatcttg
     1441 cggatatgcc caatatcgac gtgatcgacg gccaggcgga gtttatcaat aatcatagcc
     1501 tgcgtgttca tcggcctgag ggaaatctgg aaattcatgg cgagaaaatt tttattaata
     1561 ccggtgcaca aaccgtggtt ccgccaattc ctggaattac caccacgcca ggagtatatg
     1621 acagcaccgg attacttaat ctaaaagaat tgcctgggca tttaggtatt ttgggcggcg
     1681 gatatattgg cgttgagttc gcctctatgt tcgctaattt tggcagcaag gtaaccattt
     1741 tagaagcagc ttcactgttt ttgcctcggg aagaacggga tattgctgat aatatcgcga
     1801 cgattttacg cgatcagggc gtcgatatta tcctcaatgc ccatgtggag cgaatcagtc
     1861 accatgaaaa tcaagtgcaa gtgcatagcg agcacgccca actggcggtg gatgcactgt
     1921 taatagcttc cggtcgtcaa ccggctaccg cttcgttaca tccagaaaat gccggtatcg
     1981 cagtaaacga gcgcggggca attgtcgttg acaagcgatt acataccacc gcagacaata
     2041 tttgggcgat gggagatgtt accggcgggc tgcaatttac ttacatatca ctggatgatt
     2101 accgcattgt acgtgatgag ttactgggtg aaggcaaacg tagtactgat gatcggaaaa
     2161 atgtgcctta ttccgtattt atgacaccgc ccctgtccag ggttggtatg acagaagaac
     2221 aagccagaga gagtggtgct gatattcagg tggtgacatt gcctgtagct gcaattccgc
     2281 gcgccagagt gatgaacgat acccgtgggg tattaaaagc gattgttgat aataaaaccc
     2341 aacgtatatt aggggcatca ctgctgtgtg ttgactccca cgagatgatt aatatagtga
     2401 aaatggtgat ggatgccggg ctgccttata gcatattacg cgatcagata tttactcatc
     2461 cgtcgatgag cgaatcactc aatgatctat tttcattagt caaataaact caaaatcaga
     2521 cgccagaaca aatattctgg cgtctcagag aaaagaatct tattaat
//
LOCUS       DBANUU010000310         2537 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000310 DBANUU010000000
VERSION     DBANUU010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.316"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            3..1277
                     /locus_tag="ACIE68_004853"
     CDS             3..1277
                     /locus_tag="ACIE68_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFX5911001.1"
                     /translation="MIPMKNYLFQTPEDICVQLKKMTHPVTIRTTDIANFWHYLESAT
                     LPVITKSTTTENREVTFLWRSEKALQGVYLRLNRVTDKKDVKKGLMTHIPSTDIWMLT
                     LVLPASYRGSYSFIEIPTDMTQKDIFQLGSRFSPLPGKSDPFNKTAEINIRGFGESVL
                     SLDMAPEQNEWDDTSHKCTGILSTLHSFVAGYQRRIRLYFPLNPTSVPLGLLVLPDAE
                     TWFDRIDITRALDMAITTGRITPMAIMGIDNINESDRMNILGGNKELIFDIAENLIPQ
                     LYRDYPDIVWAGRSNTVLAGQSLGGVTALMAAIYASTTFGTIISHSPSMWWNPDQGSP
                     ILFTENNTSWVSEQILSAPPKDVNIRLGVGSLEGTTVSHVQRLHHSLIAAGLESNLTV
                     YTGGHDYAWWRGAIIDELANYNYRKVSNNNSV"
     gene            complement(1419..>2537)
                     /locus_tag="ACIE68_004854"
     CDS             complement(1419..>2537)
                     /locus_tag="ACIE68_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFX5911002.1"
                     /translation="KRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANKAA
                     EAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPDLI
                     VYPPLGPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCDLA
                     WIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPMVD
                     GLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGFIH
                     HGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLLYD
                     DSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
BASE COUNT          776 a          575 c          481 g          705 t
ORIGIN      
        1 agatgatacc tatgaaaaac tatcttttcc agacacccga agatatttgt gtacagttaa
       61 aaaaaatgac acatcctgtc acaataagaa caacagatat tgctaatttc tggcactatc
      121 ttgagtcagc aacacttcca gtgatcacaa aaagcaccac tacagaaaat cgggaggtta
      181 catttctgtg gcgctcagag aaagcactgc aaggcgtata tcttcgcctg aatcgtgtta
      241 cagataaaaa agacgtcaaa aaaggactaa tgactcatat cccttcgaca gatatctgga
      301 tgctgacact ggtgttacca gcttcatatc ggggctcata ctcatttata gaaattccca
      361 cagatatgac acaaaaagac atatttcaac taggaagtcg cttctctcca ttacccggta
      421 aatctgatcc atttaacaaa acagcagaaa taaatatacg aggattcgga gaatcagtcc
      481 tttctcttga tatggctcct gaacaaaatg aatgggatga tacttcccat aaatgtacag
      541 gtattctttc aacattacat tcctttgttg caggatatca acgccggatt cgtttatatt
      601 ttcccctgaa tccaacatca gtacctcttg gattacttgt gttacctgat gctgaaacat
      661 ggtttgaccg gatagatatt acccgggcat tagatatggc catcaccact ggtcgtatca
      721 cgccaatggc aattatgggg atagacaata ttaatgaatc tgatcgtatg aatatactgg
      781 gaggcaataa agaactcatc tttgatatag cggaaaatct gataccccag ttatacagag
      841 actatccgga tatcgtatgg gctggtcgct ctaatactgt actggccggg cagagcctcg
      901 gtggagtgac agcactgatg gcagctatat atgcgtcgac aacatttggt acaatcatta
      961 gtcactcacc ttcaatgtgg tggaaccctg accaaggcag cccgatcttg tttactgaga
     1021 ataatacctc ctgggtaagt gagcagatac tttcagcgcc tccgaaagat gtaaatatcc
     1081 gacttggagt cggttcttta gaaggtacaa ccgtctcaca tgttcagcga ttgcatcatt
     1141 cgttaatcgc agctggtttg gaaagtaacc tcactgtcta taccggtggt cacgattatg
     1201 cctggtggcg cggagcaatt attgatgaat tagcaaatta taattacagg aaggtatcaa
     1261 ataataactc tgtgtaaaat atcgttttat cgtccgataa ctctttctgc aatattatta
     1321 tgggagacta tctcatttat ttagtgatgt ccttcattta tgtgatgaag gacattatat
     1381 tactcataac attcctcgtt gaaaatttca gcaatacgct acaattgttt cccgttgttt
     1441 tttaattttc tcatcaaaac ctctgcaatc tctgcaggac tgggttgttc agccatttca
     1501 gcggctacct gatctgaaca gaagcgtagt gaatcatcat aaagcaggcg atttaccaaa
     1561 tcactggtca gtccatgctt gtccggaata atcccacacc cacgcttcgc aacaatttct
     1621 gcattaacag agcgatctgc accttcgcca aacacaatct gtggtattcc actatacagc
     1681 gctgtcaggg tattacctgc gccaccgtga tgaataaatc catcagctcc attaaggaat
     1741 acacccatag gtatccagtc aaccagacgt acatttgatg gtagttttcg taaaccagta
     1801 cgagcattta ttgcaagttg caaaatgata tcagcatcaa cttcatttgc agaatccata
     1861 acccatgaaa tcagctccag accatcaacc attggtttta cagtgccaag gctgataagt
     1921 aaacgcttcc gatcagaatc cctgtcccac catgtttcct ttacagcacc tccgttataa
     1981 ggaatatatc tcattgagat aaccggttct ccagcatttt taagaatgct catacttggg
     2041 ggagcaacat caatccatgc taaatcacat agtggaccac tgactccatg ccgcctgtaa
     2101 gcattgctta aagaacggtt taacatctga atatgggcag atgtatgcgc gaatccaaca
     2161 gccagcatca ctgaaggaat tctatattta gcagcaacca atgggcctgc cggaccaaga
     2221 gggggataga ctattaaatc tggcctccat tttcctgcaa aatcgatgag gttatctgcc
     2281 atttcatcgc taaaaaaaga gaaatgacca ataatattac tttttttcct caactcttcc
     2341 tgatggcgat atccagcctc tgaatctaaa cctggaactg catcaaaaac aaccagtcca
     2401 gcttctgctg ctttattcgc gaatttgcca gcgctactaa ttactacatc atgtccaatt
     2461 acacgaaagg cctgagccag agaaatcaat gggtataaca aaccgtacag cggtgggcca
     2521 ataaaaagaa tacgttt
//
LOCUS       DBANUU010000311         2509 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000311 DBANUU010000000
VERSION     DBANUU010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.317"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>2509)
                     /gene="traG"
                     /locus_tag="ACIE68_004855"
     CDS             complement(<1..>2509)
                     /gene="traG"
                     /locus_tag="ACIE68_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFX5911003.1"
                     /translation="ALTLSVLAVAVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQI
                     IDNSDLVKVHRVDNVPVGLAMPLSLTTRIGHAMVASYEMIFTQPDSVTYSKTGMLFGA
                     ELVSKSTDFLSRNPEIANLFQDYVQNCVMGDIYLNHKYTLEELMASADPYTLIFSRPS
                     PLRGVYDSNNNFVTCKDASVSLKDKLNLDTQSGGKTWHYYAQQLFGGRPDPNLLFSTL
                     IGDSYSYFYGSSKSASQIIRQNVTINALKEGITSYAARNGDSASLVNLATTSSMEKQR
                     LAHVSIGHVAMRTLPMTQTILTGIAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQS
                     WPMLYAILNSAMTFYAKQNGAPVVLSEISQIQLKYSDLASTAGYLSMMIPPLSWMMVR
                     GLGAGFSSVYSHFASSAISPTASAAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQ
                     MMYQTGSGATATQTRDGNMVMDASGAMSRLPVGINATRQIAAAQQEMAREASNRAESA
                     LHGFSSSIASAWNSLSQFGSNRGSSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAH
                     NISNEQATRELASRSTRGSAGMYGDAHAEWGVKPKILGVGGGVGVRGGGRASIDWSDE
                     DAHQASSGSRASHDTRHDIDARATQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLS
                     AALNSAKQSYDQYTTNMTRSHEYAEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAE
                     AILTNTSSPEIAERRRAMAWSFVQEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDI
                     IADHQGHQAIIEQRTQDSNIRNDVKHQVDNMVTEYRGN"
BASE COUNT          566 a          728 c          607 g          608 t
ORIGIN      
        1 tattgcctcg gtattctgtg accatattat caacctgatg ttttacatca ttacggatat
       61 tactgtcctg cgttctttgc tcaataatgg cctgatgccc ctgatgatca gcaataatat
      121 cctggctgcc cccccccgaa ggtacgctct ccattccttt accgatgtcc ccacgggatt
      181 cacgccaggc attatcaaca ccaggctgca cctgttcctg aacaaaagac cacgccatag
      241 cccgacggcg ttctgcaatc tccggggaac tggtgtttgt cagaatagct tcggcatcct
      301 gcggcgcgtg tttcatcaca tactgtgcaa actgttgcga caggtcttca ctcatctgcc
      361 cactcatgct ttcagtacgg gatgccattt cagcatattc atggctgcgg gtcatattcg
      421 tcgtgtactg gtcgtaactc tgttttgctg agttcagggc tgcagacagc tggtccacac
      481 gggaatcggc attattgtcc gtatggctgc cggattcact gaccttgcga ctggtaaagt
      541 aatcgctggc ctctttaaag tcttgtgtag ccctggcatc gatatcatgg cgagtatcat
      601 ggctggcccg tgaaccactg ctggcctgat gcgcatcctc atcactccag tcaatacttg
      661 cccgaccacc acctctaaca cctacgccac caccaacacc aagaatttta ggtttaaccc
      721 cccattcagc atgagcatcg ccatacatcc ccgcagaacc tctcgtactt cttgatgcca
      781 gttctcttgt cgcctgctca ttactgatat tatgcgcctt cgcatagctt tccactgcgc
      841 tgcgcatacg gctggccatc atggagtcct gtgcgctcat cgtgctgtca gcaccaccgg
      901 tgacagaatc actgctcccc cggttagaac caaactgact gagcgagttc caggcactgg
      961 caatactgct gctgaaccca tgcagggcgc tttctgctct gttcgacgcc tcccgggcca
     1021 tttcctgttg tgccgccgca atctgacgcg ttgcattgat accgaccggt aaacgggaca
     1081 tcgccccgct cgcatccatc accatattac cgtcacgagt ctgtgtggcg gttgcgccac
     1141 tgccggtctg gtacatcatc tgaccaaacg acgtggtgct gttggtgctc cagctgaatc
     1201 cgttcacgtt ttccgtctgc atgttgccgt aggagtaatt accgtcaacc acactacctg
     1261 ccgcactggc agtcgggctg atagcagagg aagcgaaatg gctgtacaca ctggaaaatc
     1321 cggccccgag tcccctgacc atcatccagg ataatggcgg gatcatcatg gaaagatacc
     1381 cggcagtgga agccagatcg gagtatttca gctgtatctg tgaaatttcc gagagcacca
     1441 ccggtgcacc attctgcttc gcataaaatg tcatggcgct gttcagaatg gcgtacaaca
     1501 ttggccatga ctgcaaccac atcagggcaa acacatagcc tttcaggacg gacagcgtca
     1561 gtttgttgaa gacagccgcc agtaccagaa gcgggaaaat accgatggcg atccccgtca
     1621 ggatggtctg cgtcatgggc agtgtccgca tcgccacatg accgatggag acatgtgcca
     1681 gacgttgctt ctccatcgat gacgtggtgg ccagattcac caggctggct gaatcaccat
     1741 tacgcgcggc ataactggtg atcccctcct tcagcgcatt gatggtgaca ttctggcgga
     1801 tgatctggct ggctgattta cttgagccat agaagtaact gtaactgtcg ccaatcagcg
     1861 tactgaacaa caggttcgga tcagggcgtc caccaaatag ctgctgggca taataatgcc
     1921 aggtttttcc gccactctgt gtatccagat tcagcttgtc tttcagcgaa accgacgcgt
     1981 ccttacaggt cacgaaatta ttattgctgt cataaacgcc ccgcagcggg ctggggcggg
     2041 aaaaaatcag cgtgtaggga tcagcggagg ccatcagctc ctccagcgtg tatttgtggt
     2101 tcaggtaaat atcccccatc acgcagttct gcacataatc ctggaaaaga ttggcgattt
     2161 ccggattgcg ggacaaaaag tcggtgcttt ttgataccag ttctgcgcca aacagcatcc
     2221 ccgttttgct gtaggtgaca ctgtccggtt gcgtgaagat catctcgtaa ctggccacca
     2281 ttgcatgccc gatacgggtc gtcagtgaaa gtggcatcgc cagtccgacc ggtacattat
     2341 ccacccggtg aactttgacc aggtcactgt tatcaataat ctgcaccgat gtgcggacat
     2401 taaccagcag gctgataagc acaaacacgg ccacccagcc cagcaaatcc atcacgttgt
     2461 gccgctgtac ccacatcacg gccaccgcga gaacagacaa tgtgagcgc
//
LOCUS       DBANUU010000312         2487 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000312 DBANUU010000000
VERSION     DBANUU010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.318"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            103..1035
                     /locus_tag="ACIE68_004856"
     CDS             103..1035
                     /locus_tag="ACIE68_004856"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFX5911004.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(1076..>2487)
                     /locus_tag="ACIE68_004857"
     CDS             complement(1076..>2487)
                     /locus_tag="ACIE68_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HFX5911005.1"
                     /translation="GVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKKKGEHAYD
                     MTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPITMLMHGN
                     VIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAPAEYGKFS
                     FGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLEEKKFPVG
                     ESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLTKGNQQLG
                     SGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAALFKEYLPL
                     LQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVKLPLNVAT
                     ETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRYTPGKVVF
                     NGQEMSEEEFMSRAGRFVH"
BASE COUNT          643 a          550 c          520 g          774 t
ORIGIN      
        1 taatgtggat agatatgaat tatttttctc cttaaggatc atccgttatt tgggtcgttt
       61 tctgctaaaa tctgcgcctt gcgcttcttt agcgcactgt ctatggctaa ccttttgaat
      121 aaattcatta tgacgagaat actcgctgcg ataacccttt tgctgagtat cgtattgacc
      181 attttggtca ctattttttg ttccgtcccg atcatcattg ccgggattgt aaaactattg
      241 ctacctgtgc cagtcatttg gcgaaaggtt tcgcgttttt gtgattttat gatgtattgc
      301 tggtgtgaag gtctggcggt attactgcat ctaaacccac acctgcaatg ggaagttcac
      361 gggctggaag ggttaagtaa gaagaactgg tatctgctta tttgtaacca ccgtagttgg
      421 gcagatattg tcgtactgtg cgtgctgttt cgtaagcaca ttccgatgaa taaatatttc
      481 ctcaagcaac agctggcctg ggtgccattt cttggcctgg cgtgctgggc gctggatatg
      541 ccgtttatga agcgttattc ccgcgcttat ttattacgtc atccggagcg acgtggtaaa
      601 gatgttgaaa ccactcggcg ttcttgtgaa aagtttcgcc tgcatcccac caccattgtc
      661 aatttcgttg aaggctcccg tttcacgcaa gaaaaacatc agcaaaccca ctccactttt
      721 caaaacttgt tgccaccaaa ggcggcaggc attgcgatgg cgctaaatgt actgggtaaa
      781 caattcgata aactgcttaa tgttacgctg tgttatccgg acaataaccg tcagccgttc
      841 ttcgatatgt taagcggtaa actgacgcga attgtcgttc atgtggattt acagcctatt
      901 gccgatgagt tacatggcga ctacatcaat gacaaaagct ttaagcgcca ttttcagcaa
      961 tggctgaact cactgtggca ggaaaaagac cgacttctga cgtcgttgat gtcatcacag
     1021 cggcaggaca agtaaatttt gctcgcctct gaatgagcag aggcgagtga gtattttaat
     1081 gaacaaaacg tccggcacga gacataaatt cttcttcgct catctcctgt ccgttaaaaa
     1141 caactttacc cggtgtataa cgcagttgca gcgaggcggt attgttgtca atcgtgatta
     1201 actgaaacat ctgacctaag gtcatcatcc cgctgatttg tttatcagcc cgcttttgcg
     1261 ctttttccgc atccattcct tcagataaat taagctgttt tgcggtttct gtggcgacat
     1321 taagcggtaa cttcacatca aaattgagcg atttaatatc tttgtttgtg gatgatgaag
     1381 atttggctgg gtcggcaata ctgatatcca gattggcatt tagttcgccg agtgcgttct
     1441 tccattttac cggttgttta atggtcggct cacttttttg taataacggt aaatactctt
     1501 tgaacaatgc agcattcact tcttgcagag caacttcatc gtttgctaac tcagggtgtg
     1561 cagcaagctg cttctgcatt gcaatgttat actggatgat aaattggcgc accgctgagg
     1621 gatcaataga ttcagcaatc aatgaccact caccactacc gagttgctga ttcccttttg
     1681 ttaacttatc aagttcgtag gtcagattga cattgatata atctttgtct ttaacgcgat
     1741 ccagcatggt ttttgcaacg aaagcatcga tttgggcaac gtcttccccg tgattgataa
     1801 tgttaatctg attaaatttc gactcgcttt caccaaccgg aaatttcttt tcttctaatc
     1861 gcgccagaga atcaatggta aaactttttg ccgttacttt gttcattggt gataattgca
     1921 gaacaatatc ttcgactttg ccttcaatat ccaggttaga caatgaacta ccatcaccat
     1981 taaaagtaaa ctggccctca ccaaaagaaa actttccata ctctgcaggg gcaacatcta
     2041 atgtcgtaga gaatgagcca ccaaacgcga atcgcaatgt cgcttccacg ggcgatttat
     2101 ttttcgcggc gataaatagc ggttgtgtca gctcattatt cactaagttc acttttgccg
     2161 ctgccagtgc tgggatgaca ttaccatgca ttaacattgt gatgggcaac ggaccgtgct
     2221 caacatccac atcaaataca actttctggc ctggcttgat attgagatcg ggtgcaccgt
     2281 tatcgaatgt catttgcatt tgaaaacgag aattaaaaaa acctttgtca aaatttttat
     2341 aacttaacgt catatcgtag gcatgttcac ctttcttttt agcatcgtta aaatctttaa
     2401 taaacttttc gacaccaggc tgaatttgcg tacctgtgta ccatgtgcca ccaccccaga
     2461 ttacggctaa cgcaacaata acacctg
//
LOCUS       DBANUU010000313         2486 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000313 DBANUU010000000
VERSION     DBANUU010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.319"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..186
                     /locus_tag="ACIE68_004858"
     CDS             <1..186
                     /locus_tag="ACIE68_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911006.1"
                     /translation="GLIYNLGATGGMAAPVLATYISGYYGLGVSLFIVTVAFSALLIL
                     LVGFDIPGKIYKLSVAK"
     gene            198..1316
                     /gene="nanY"
                     /locus_tag="ACIE68_004859"
                     /gene_synonym="yjhC"
     CDS             198..1316
                     /gene="nanY"
                     /locus_tag="ACIE68_004859"
                     /gene_synonym="yjhC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,7-anhydro-N-acetylneuraminate hydratase"
                     /protein_id="HFX5911007.1"
                     /translation="MINYGVVGVGYFGAELARFMNMHDNAKITCVYDPENGENIAREL
                     QCINMSSLDALVSSKLVDCVIVATPNYLHKEPVIKAAKNKKHVFCEKPIALSYEDCVD
                     MVKACKEAGVTFMAGHIMNFFNGVQYARKLIKEGVIGEILSCHTKRNGWENKQERLSW
                     KKMKEQSGGHLYHHIHELDCVQHLLGEIPETVTMIGGNLAHSGPGFGNEDDMLFMTLE
                     FPSGKLATLEWGSAFNWPEHYVIINGTKGSIKIDMQETAGSLRIGGQTKHFLVHETQE
                     EDDDRRKGNMTSEMDGAIAYGHPGKKTPLWLASLIRKETLFLHNILCGAKPEEDYIDL
                     LNGEAAMSAIATADAATLSRSQDRKVKISEIIKHTSVM"
     gene            1359..1484
                     /gene="ythA"
                     /locus_tag="ACIE68_004860"
     CDS             1359..1484
                     /gene="ythA"
                     /locus_tag="ACIE68_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YthA"
                     /protein_id="HFX5911008.1"
                     /translation="MIKNFIFDNLIILAVPFMIKTSLKTNLIFFFLCVFVPHMAS"
     gene            complement(1537..1794)
                     /locus_tag="ACIE68_004861"
     CDS             complement(1537..1794)
                     /locus_tag="ACIE68_004861"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFX5911009.1"
                     /translation="MVSSSASNVVNCETKQRTQFECIYFSQYWAKGDFIAKRAPIGQW
                     EPYSEESLLGIIVTSVCRIKVAMLKPEPPRDPHIPLMGDFN"
     gene            1817..2020
                     /locus_tag="ACIE68_004862"
     CDS             1817..2020
                     /locus_tag="ACIE68_004862"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5911010.1"
                     /translation="MDITPKYVGIASGLMNAGSAVADIISPIAFGIIIDKTGNWSLPF
                     YGSVALLVIGIFLTFFMRPDKSL"
     gene            2108..2486
                     /locus_tag="ACIE68_004863"
                     /pseudo
     CDS             2108..2486
                     /locus_tag="ACIE68_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          753 a          472 c          558 g          703 t
ORIGIN      
        1 ggtcttattt ataacttagg ggcaactgga ggaatggccg cacctgtatt agctacatac
       61 atttcaggat attatggctt aggtgtttca ttattcattg ttacggttgc attctctgcc
      121 ttattaattt tgttagttgg ttttgatatt ccaggtaaaa tttataaact atccgtggct
      181 aaatgattgg aggctttatg attaattatg gcgttgttgg tgttggatac tttggcgctg
      241 aattagctcg ttttatgaat atgcatgata atgcaaaaat tacatgtgta tacgatcctg
      301 aaaatggaga aaatattgcc cgtgaattgc agtgtatcaa tatgtcaagc ttggatgctt
      361 tagtctcaag taaattagtc gattgcgtga tcgtagccac cccaaattat ctgcataaag
      421 aaccagtaat taaagcagca aagaataaga agcatgtttt ttgtgaaaaa ccaattgcat
      481 taagttatga agattgtgtg gatatggtca aagcgtgtaa agaagctggt gtgaccttta
      541 tggccgggca tattatgaat tttttcaatg gggttcaata tgcacggaag ttaattaaag
      601 aaggtgttat cggcgaaata ttatcatgtc atactaagag aaatggctgg gaaaacaaac
      661 aagagagact ttcctggaaa aagatgaaag aacaatctgg tggacatcta tatcatcata
      721 tacatgagtt agattgtgtt cagcatttac ttggagaaat accagagacg gttactatga
      781 ttggtggaaa tttggcccat tctggtccag gatttggcaa tgaagatgat atgttattta
      841 tgaccttgga attcccgtca ggaaaactag caaccttaga gtgggggagt gcatttaact
      901 ggccggaaca ttatgtcatc atcaatggaa ctaaaggctc tattaaaatt gatatgcaag
      961 aaacagcagg gtcacttagg attggcggtc agacaaagca ttttttggtc catgaaacac
     1021 aagaagaaga tgatgatcgt cggaaaggca atatgacctc agaaatggat ggcgctatag
     1081 catatggtca tccaggaaaa aaaacaccat tatggcttgc cagtttaata agaaaggaga
     1141 cgttattcct ccataatatc ctctgtggtg caaaacctga agaagattat attgaccttc
     1201 tcaatggtga ggcggccatg tcggcgattg ctactgctga tgctgccact ctttcaagat
     1261 cgcaggacag gaaagtgaaa atcagtgaga tcattaaaca tacatcagta atgtaaaaac
     1321 tatacgcctc ttgatttaat tcaggaggcc ttttatgtat gatcaaaaat tttatcttcg
     1381 ataatctcat aattttagca gtgccattta tgataaaaac atcccttaaa accaatctaa
     1441 tctttttctt cctctgtgta tttgttcccc atatggcgtc ataatttacg ctacgtaata
     1501 cgggatagtt acgatacgca gcgatagcgc taagttttag ttaaaatccc ccatcagcgg
     1561 gatatgggga tctctgggag gttcaggttt tagcatcgcg actttaatac gacagacgct
     1621 ggtgacgatg atgccaagta gtgactcttc cgaataaggt tcccactgac caattggcgc
     1681 gcgcttcgca ataaaatctc ccttcgccca gtattgggaa aagtagatac attcaaactg
     1741 tgtacgctgt ttcgtctcac agttcacgac attgctggcc gatgagctga ccattgggcc
     1801 tatccgggct gtcccgatgg atattacgcc gaagtatgtg ggaattgcca gcggattgat
     1861 gaacgccggt tccgctgtcg ccgacattat ttcacctatc gcctttggca ttattatcga
     1921 caaaacaggc aactggagtt taccattcta cggttccgtc gcattgcttg ttattggtat
     1981 attcctgacg ttcttcatgc gtccagataa gtcattgtaa aagtcgaatc agggctgaag
     2041 tggcacactg aatttggcca cctgaacaga ggtgatatgc tcacctcaga acaacacagg
     2101 tgctccaatg aaaaaaagaa atttcagcgc agagtttaaa cgcgaatccg ctcaactggt
     2161 tgttgaccag aaatacacga tggcagatgc cgcgaaagct atggatgttg gcctttccac
     2221 aatgacaaga tgggtcaaac aactgcgtga tgagcgtcag ggcaaaacac caaaagcctc
     2281 tccgataaca ccagaacaaa tcgaaatacg taagctgagg aaaaagctac aacgcattga
     2341 aatggagaat gaaatattaa aaaggctacc gcgctcttga tgtcagactc cctgaacagt
     2401 tctcgataat cgggaaactc agagcgcatt atcctgtggt cacactctgc catgtgttcg
     2461 gggttcatcg cagcagctac agatac
//
LOCUS       DBANUU010000314         2480 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000314 DBANUU010000000
VERSION     DBANUU010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.320"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..436
                     /locus_tag="ACIE68_004864"
     CDS             <1..436
                     /locus_tag="ACIE68_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911011.1"
                     /translation="LTAQGKTVWPSLLEEASQHDDAWLEQEILNRGLLATHYPRRKPN
                     STEIIQAKVPHTAARTLAEGEFNRLFARGLSSRAIAEGIEFVEAYRARHSENPRPESQ
                     AVIGKKFRPEEILEDLRNNPGVDTALGVPPGPNSGITIKLVK"
     gene            980..1198
                     /locus_tag="ACIE68_004865"
     CDS             980..1198
                     /locus_tag="ACIE68_004865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911012.1"
                     /translation="MDVKADIIAWLLKQQDWFQELAERLIQQDDLTDNDLQQVIALLK
                     PPPMDEQKRHTILSQAWHLQSRDLEHSG"
     gene            1597..>2480
                     /gene="istA"
                     /locus_tag="ACIE68_004866"
     CDS             1597..>2480
                     /gene="istA"
                     /locus_tag="ACIE68_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HFX5911013.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLEACHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLE"
BASE COUNT          670 a          597 c          596 g          617 t
ORIGIN      
        1 tcttactgct caaggtaaga ctgtgtggcc ttctttgtta gaagaagcat ctcaacatga
       61 tgatgcatgg cttgagcaag aaatcctaaa tagaggatta ctggccaccc attatcctag
      121 aagaaagcct aatagcactg agataattca ggctaaagtt cctcacacag ctgctcgaac
      181 tttagctgaa ggagaattca atcgtttatt tgcccgaggc ttgagctcgc gagcaatagc
      241 agaaggaatt gaatttgttg aagcttaccg agcaagacac tcagaaaatc cacgccctga
      301 atctcaagct gttatcggga aaaagttccg accggaagaa attcttgagg atttacgtaa
      361 taacccaggt gtagatacag cattaggcgt tcctcctggc cctaactcag gcataacaat
      421 taaacttgtt aagtaaaaaa ttaataagag gcactttcgg aatttaaatc cctaagtgcc
      481 tttatgatga cctgttcccc cattgttcaa cacacactga tctaagaaag cttggttgca
      541 gacaggccat gaataacacg gttactgtga gcgagcagcg gacggcttag gttttatgat
      601 actgatcaag gctaatatca tggcgatgaa atcattcgga taattttaat ctcaccttta
      661 tatatcactg cccagtcgcc aaaagttagg atacttatgc ctcgtctgca aacctgtagc
      721 gaagtacccg cagacgttca cttcccggac ttttcaggta gtgagcagac ttcatttcgg
      781 gatctactgg agcgtgagca ttttactgtg gattacgaat acactattgc caatttttgt
      841 tgatcaagtg ttgctcatct caagtggctg atgcactatc agatgattct taacgccaca
      901 cactagggcg tggtagcaat gtgctgggag gcacattcag tttcgcttcc tttatcgtca
      961 tgaatgaagg agggcagtaa tggatgtaaa ggccgatatc atagcctggc tgttaaagca
     1021 gcaggactgg tttcaggaac tagcggaaag actcattcaa caggatgacc tgacagataa
     1081 tgaccttcag caggttattg ctttgctcaa gccccccccc atggacgaac aaaaacgaca
     1141 caccatactt tcccaggcct ggcatctgca gagcagggat ctggaacact caggttaagt
     1201 cggatcgaag ctgtaaaggg tattgagaac cttgctccac gtttaccgct cgatttcggt
     1261 tcagggaatc tgactgttat ttacggccat aacgggtccg gtaaatccag ctatacgcgt
     1321 gtgctcaaac gtatgtccgg aaaacctaga gcagctcagc ttaaatctaa tgtctttaat
     1381 cccttaccac ctgagcgaag ttgccacatt aaatggaaag cgaatgaaag caatgctgaa
     1441 gcggactggt cagctgacag ccctccgatc gatgccctga aaggcattga tatcttcgat
     1501 acggatgaag tgtcacgaac ggtgcaatag tgatccacac ccaacgcctg aaatcagatc
     1561 cagggggtaa tctgctctcc tgattcagga gagtttatgg tcacttttga gacagttatg
     1621 gaaattaaaa tcctgcacaa gcagggaatg agtagccggg cgattgccag agaactgggg
     1681 atctcccgca ataccgttaa acgttatttg caggcaaaat ctgagccgcc aaaatatacg
     1741 ccgcgacctg ctgttgcttc actcctggat gaataccggg attatattcg tcaacgcatc
     1801 gccgatgctc atccttacaa aatcccggca acggtaatcg ctcgcgagat cagagaccag
     1861 ggatatcgtg gcggaatgac cattctcagg gcattcattc gttctctctc ggttcctcag
     1921 gagcaggagc ctgccgttcg gttcgaaact gaacccggac gacagatgca ggttgactgg
     1981 ggcactatgc gtaatggtcg ctcaccgctt cacgtgttcg ttgctgttct cggatacagc
     2041 cgaatgctgt acatcgaatt cactgacaat atgcgttatg acacgctgga ggcctgccat
     2101 cgtaatgcgt tccgcttctt tggtggtgtg ccgcgcgaag tgttgtatga caatatgaaa
     2161 actgtggttc tgcaacgtga cgcatatcag accggtcagc accggttcca tccttcgctg
     2221 tggcagttcg gcaaggagat gggcttctct ccccgactgt gtcgcccctt cagggcacag
     2281 actaaaggta aggtagaacg gatggtgcag tacacccgta acagttttta catcccacta
     2341 atgactcgcc tgcgcccgat ggggatcact gtcgatgttg aaacagccaa ccgccacggt
     2401 ctgcgctggc tgcacgatgt cgctaaccaa cgaaagcatg aaacaatcca ggcacgtccc
     2461 tgcgatcgct ggctcgaaga
//
LOCUS       DBANUU010000315         2465 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000315 DBANUU010000000
VERSION     DBANUU010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.321"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            324..467
                     /locus_tag="ACIE68_004867"
     CDS             324..467
                     /locus_tag="ACIE68_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFX5911014.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            480..689
                     /gene="cspE"
                     /locus_tag="ACIE68_004868"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             480..689
                     /gene="cspE"
                     /locus_tag="ACIE68_004868"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFX5911015.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            855..1664
                     /gene="rlmA"
                     /locus_tag="ACIE68_004869"
     CDS             855..1664
                     /gene="rlmA"
                     /locus_tag="ACIE68_004869"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFX5911016.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1661..2227)
                     /gene="mntP"
                     /locus_tag="ACIE68_004870"
     CDS             complement(1661..2227)
                     /gene="mntP"
                     /locus_tag="ACIE68_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFX5911017.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          651 a          589 c          603 g          622 t
ORIGIN      
        1 gagaaaaagt gatataactt tttattcatt gcatagcaaa aaatgtgata ttgcacgcac
       61 tatgtaataa cttctcccac tggcctggaa caactgaact tattgaacta tgttagaaac
      121 tacgccagtt taagtatctg cctgaactgg caaggttaag cacaatgata tatcggcgcg
      181 tattccgttg cataagtgtg caaaaaaagt ggaagacgta tcgagatttg tgcgtctgat
      241 cgagacatgt ttaaaaatgg cttgccataa ttaacgttgt atgtgataac agatttcggg
      301 ttaaacgagg tacagttctg tttatgtgtg gcattttcag taaagaagtc ctgagtaaac
      361 acgttgacgt tgaataccgc ttctctgccg agccttatat tggtgcctca tgcagtaatg
      421 tgtcagtttt atctatgtta tgcctgcggg cgaagaaaac aatctaagga atttttcaaa
      481 tggcaaagat taaaggtcag gttaagtggt tcaacgagtc taaaggtttt ggcttcatta
      541 ctccggctga tggcagcaaa gacgtgttcg tacacttctc cgctatccag ggtaatggct
      601 tcaaaactct ggctgaaggt cagaacgttg agttcgaaat tcaggacggc cagaaaggtc
      661 cggcagctgt taacgtaaca gctatctgat cgaatccact gatctgaagt gtgaatacgc
      721 ttcaatctcg ctataaagcc tcgtcgaatg cgaggctttt tactatgctt tatcttcgct
      781 cctggcgttc ggatatttgc ccgccgcgtg attcgcgtta cacttgcggc ctttagtatc
      841 ctgccggagt tgtcatgtct ttttcctgtc cactttgcca tcagcctctt tcgcgtgaaa
      901 aaaacagcta tatctgtccc cagcgacatc agtttgatat ggcgaaagaa gggtatgtca
      961 atctgctgcc cgttcagcat aaacggtctc gtgatccggg cgacagcgcg gaaatgatgc
     1021 aagcacgccg cgcattctta gatgccggac attatcagcc gctgcgtgat gcaattgtcg
     1081 cccaactgag ggaacggctt gatgataagg ccacggcggt gctggatatt ggctgtggtg
     1141 aagggtatta cacacacgca tttgccgatg cgttgcccga aatcaccacg tttggtctgg
     1201 atgtttcgaa ggtagcgata aaagcggcgg cgaaacgcta tccgcaggtc actttttgtg
     1261 tcgcttccag ccaccgtttg ccgttttccg ataccagtat ggacgccata atacgtattt
     1321 acgcgccgtg taaagcagaa gaattagcac gagtagtgaa gcccggcggc tgggtcatta
     1381 ctgccacgcc gggaccgcga catttgatgg agctgaaggg gctgatttac aatgaagtac
     1441 atcttcatgc acctcatgca gaacaactgg aaggttttac attacagcag agtgcggagt
     1501 tgtgttatcc gatgcgtctt cgcggtgatg aagccgtcgc attattgcag atgacgccgt
     1561 ttgcctggcg tgcgaagcca gaagtctggc aaacactggc agcaaaagaa gtgttcgact
     1621 gccagacgga ctttaatatt cacctctggc agcgttctta ttaaccgtgg aagtgcgtcc
     1681 agaggatctg gacgccgatg ccgatcagta ccagcccgcc gagaatttcc gcttttttcc
     1741 caataattga gccgataaag cgaccaacca tcatccctaa tgttgacata atcaaggttg
     1801 cacaaccaat ggccaatgcg gtcgcgataa tgttgacctg caggaaagca agaccaacac
     1861 ccacagccat ggcatccagg ctggtggcaa tcgcggtggt taccagtagc cagaaaccgt
     1921 gtcgacggcg cggctcttca tcttcatcat ctgcgccacg aaaaccctca ataatcattc
     1981 gcccgccgag gaatatcagc agcacaaacg caatccagtg gttccattca aggacaaacc
     2041 ggctggctaa catgcccatt ccccagccga tcagcggcgt cagggtttcg acggcaccaa
     2101 aaataaggcc ggttcgcaat gcttcagaaa atttcggttt atggagggtg gcacctttac
     2161 cgattgatgc agcaaatgca tccatcgaca taccaaacgc aagaagaaca gtagcagtga
     2221 tattcatgac aatgtcctga ccggggtatc cataaaacac atcatcgccc ccagcaaaca
     2281 tgcatacatg aacaacaagc cgcgatggtt ttcgcgttca tgcaggcata gcgattgatg
     2341 tgcatatggt ctcgcctgac tgaaagtatt cagtccgtac gtgccacgtt ttgcaacgag
     2401 tatgttgaca cgtacatttc cggaatctgg aaatcggcta ctccccaaat gagggcgcaa
     2461 cctta
//
LOCUS       DBANUU010000316         2365 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000316 DBANUU010000000
VERSION     DBANUU010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.322"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            497..1528
                     /gene="repA"
                     /locus_tag="ACIE68_004871"
     CDS             497..1528
                     /gene="repA"
                     /locus_tag="ACIE68_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFX5911018.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALKGQLGHAYNRLVKKDPVTGEQSLRMRMSR
                     HPFYVQKRTFVGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVTRLAEEISPKD
                     SEGHVIPELEVTVSRLSRLLAEQVRFGVLGVSEETMWDREHRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKKR
                     REKAAASKRANRLKKLPVDQQIYEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
BASE COUNT          647 a          628 c          610 g          480 t
ORIGIN      
        1 atcgtgagta ggacaaaagt cgatctttta atccaaaaga aagatctaaa gcagaaaaat
       61 ctgcttttaa aaatagattt tatttttttg acgcaggtca agattaactt attagaggta
      121 tcagtgagga ggcactggaa gagaagagat cgttgtaatg cttttcaaat taacgtaaag
      181 cgggtatatt tcggctgtta ttagctgtgc agagggtggc actctgtgga gcaaagcggc
      241 gaaagccgga cggcagaatg cgccataagg cattcaggag agatggcatg tacgggcagt
      301 aagtcagaag actgaagatg ttccggaagc cataaaagga aaacccccac tatctttctt
      361 acgaacttgg cggaacgacg aaagatagtg ggggcctcac agaatacggg taaagtataa
      421 tgaaaccgta ccagagattc aaccctgtgc agtgtataaa tacacggcac aatcgctccg
      481 ccataagcga cagcttgtgg caggtctgaa gaatactcca tataacgcag tacactggag
      541 tcagttagca cccgaagagc agatccgttt ctgggaagac tatgaagcgg gaagggcgac
      601 cactttcctg gttgaaccgg aaaggaagcg cacgaagcgc cgtcgcggtg agcactccac
      661 caaacccaaa tgcgaaaatc cgtcctggta tcgtcctgag cgctataaag cgctgaaagg
      721 gcagcttggg cacgcctata accgtctggt gaaaaaggac ccggtgaccg gcgagcagag
      781 cctgcgcatg cgcatgtcgc ggcatccttt ttatgtacag aaacggacgt tcgtcggtcg
      841 caaatacgct ttccggccgg aaaaacaacg tctcctcgat gccatctggc cggttctggt
      901 cagcttcagt gatgccggca cacataccgt ggggatgagc gtcactcggc tggctgaaga
      961 aatcagcccg aaagacagcg agggacatgt tattccggaa ctggaagtga cggtctcccg
     1021 tctttcccgt ctgctggccg aacaggtgcg ctttggtgtg ctgggtgtgt cagaggaaac
     1081 aatgtgggac cgtgaacatc gtcagcgtct gccacgttac gtctggataa caccggcagg
     1141 ctggcagatg ctgggcgtcg acatggtaaa acttcacgaa cagcagcaga aacgactgcg
     1201 tgaaagtgaa atccgccagc agctcattcg ggaaggtgtt ctgcgtgagg atgaagatat
     1261 ctccgttcat gcggccagaa aacgctggta tctgcagcgc agccaggatg ccctgaagaa
     1321 gcgccgagaa aaagccgcag ccagcaagcg tgccaaccgc ctgaagaaat tacctgttga
     1381 ccagcagatt tatgagatgg ctgagtatct ccggaagcgt ctgccgccgg atgaagccta
     1441 tttctgttcc gatgaccatc tgaagcgact ggccatcagg gagttgcgtc agcttgaact
     1501 gacgctggct gccccgccac cgcactagac agcatcattc cctcagcact gaatcatcgc
     1561 cagcccctcc ggggctttcg gcgcagattc cgctcagccc aaaatcctta gtagtcacct
     1621 taaatcccct cagaggggca tatctgccca taaaaccacg catcagtcat cagaacatgg
     1681 ccacgttgtt tcagttatcc acataaatcc gcaaacaaag aactttaaga agctgcaaac
     1741 ctaaaacagc aaacctgcaa tatagtctta accccattat ttaatcccct gcgttgcttc
     1801 gccgcaggga aaatctttat ctctgagacc actgtgaaca aatacaaaga ggccttcgct
     1861 tgcagcggcc aaggccgcgc cgctcagaat ctaaaagcac ctcccacgct gacgcgcggg
     1921 ccccgaacct caccgttctg aaaccacaac aaaaaaacat caggaataaa aacaccacac
     1981 aaacgcagca ccgtgcccac ccctcataac tgaaaagcga ggccgccccc gcccaaaggg
     2041 cgggaacaac atcgctttta attatgaatg ttgtaactac attgtcatcg ctgccagtct
     2101 tctggctgga agtcctcagt acacgctcgt aagcggccct tacggcccgc taacgcggag
     2161 atacgccccg actgcgggta aacccttgtc gggaccactc cgaccgcgca cagaagctct
     2221 gtcatgactg aaaacgagta tggtctagca gggctgggga tgggtaaggt gaaatctatc
     2281 aatccgtacc ggcttacgcc gggcttcggc ggttttactc cggtatcaca tgtaaccaca
     2341 gcgtgccgcc ttccatgccg ctgac
//
LOCUS       DBANUU010000317         2347 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000317 DBANUU010000000
VERSION     DBANUU010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.323"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(147..356)
                     /gene="fumD"
                     /locus_tag="ACIE68_004872"
     CDS             complement(147..356)
                     /gene="fumD"
                     /locus_tag="ACIE68_004872"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFX5911019.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            711..905
                     /locus_tag="ACIE68_004873"
     CDS             711..905
                     /locus_tag="ACIE68_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911020.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            913..2325
                     /gene="pykF"
                     /locus_tag="ACIE68_004874"
     CDS             913..2325
                     /gene="pykF"
                     /locus_tag="ACIE68_004874"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFX5911021.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          658 a          571 c          505 g          613 t
ORIGIN      
        1 gcagaaaata aaaataacat tatcgctata aattaaaata cataccgaaa ttcgtataat
       61 ttcagcagtt gttatttaga atgattaact ggaccgcaac tgaagcaaga atatcaggat
      121 gaagcttacc gcctcatcct gaatggtcat tttaccagtg cgttaatgac ggtttccatc
      181 gcctgctttt ccagctcact gcgctgaatg cgtttattgg ctaatgctgt acgtaatacg
      241 cctgcgataa caatatgctt tggttcctgc cccagatcgc gcatttccag cacaacttta
      301 ccgacaactc tgcacatttc tcggtacagt tcatcctctt ttgtccgatt tcccattctt
      361 tatacctatt tatcatgcca actatcagca tatatcaatc taaccaatta aacaaaaatc
      421 aaacaaaatc agacaaataa cgcgataaat tattttaact gtagcaattg agcgatgata
      481 tatttataca ccggatgaac tttcacttat cctcacactg acaacttcgg caccagacgt
      541 tgcgcaaaca gtgaagtttt tgcgtaacct tttccctgga acgttaaatc tttgataaca
      601 atttattgtc taacaagttg tatatttttt gaaacgctgt ttttgttttc cttttggatt
      661 aatttcagcg tataatgcgc gccaattgac tcttgaatgg tttcagcact ttggactgta
      721 gaactcaacg actcaaaaac aggcactcac gttgggctga gacacaagca cacattcctc
      781 tgcacgcttt ttcgatgtca cctatcctta gagcgaggca ccaccacttt cgtaataccg
      841 gattcgcttt ccggcagtgc gcccagaaag caagtttctc ccatccttct caacttaaag
      901 actaagactg tcatgaaaaa gaccaaaatt gtttgcacca tcggaccgaa aaccgaatct
      961 gaagagatgt tagctaaaat gctggacgct ggcatgaacg ttatgcgtct gaacttctct
     1021 catggtgact atgcagaaca cggtcagcgc attcagaatc tgcgcaacgt gatgagcaaa
     1081 actggtaaaa ccgccgctat cctgcttgat accaaaggtc cggaaatccg caccatgaaa
     1141 ctggaaggcg gtaacgacgt ttctctgaaa gctggtcaga cctttacttt caccactgat
     1201 aaatctgtta tcggcaacag cgaaatggtt gcggtaacgt atgaaggttt cactactgac
     1261 ctgtctgttg gcaacaccgt actggttgac gatggtctga tcggtatgga agttaccgcc
     1321 attgaaggta acaaagttat ctgtaaagta ctgaacaacg gcgacctggg cgaaaacaaa
     1381 ggtgtgaacc tgcctggcgt ttccattgct ctgccagcac tggctgaaaa agacaaacag
     1441 gacctgatct ttggttgcga acaaggcgta gactttgttg ctgcttcctt tattcgtaag
     1501 cgttctgacg ttatcgaaat ccgtgagcac ctgaaagcgc acggcggcga aaacatccac
     1561 atcatctcca aaatcgaaaa ccaggaaggc ctcaacaact tcgacgaaat cctcgaagcc
     1621 tctgacggca tcatggttgc gcgtggcgac ctgggtgtag aaatcccggt agaagaagtt
     1681 atcttcgccc agaagatgat gatcgaaaaa tgtatccgtg cacgtaaagt cgttatcact
     1741 gcgacccaga tgctggattc catgatcaaa aacccacgcc cgactcgcgc agaagccggt
     1801 gacgttgcaa acgccatcct cgacggtact gacgcagtga tgctgtctgg tgaatccgca
     1861 aaaggtaaat acccgctgga agcggtttct atcatggcga ccatctgcga acgtaccgac
     1921 cgcgtgatga acagccgtct cgagttcaac aatgacaacc gtaaactgcg cattaccgaa
     1981 gcggtatgcc gtggtgccgt tgaaactgct gaaaaactgg atgctccgct gatcgtggtt
     2041 gctacccagg gcggtaaatc tgctcgcgca gtacgtaaat acttcccgga tgccaccatc
     2101 ctggcactga ccaccaacga aaaaacggct catcagttgg tactgagcaa aggcgttgtg
     2161 ccgcagctgg ttaaagagat cacttctact gatgatttct accgtctggg taaagaactg
     2221 gctctgcaga gcggtctggc acacaaaggt gacgttgtag ttatggtttc tggtgcactg
     2281 gtaccgagcg gcactactaa caccgcatct gttcacgtcc tgtaatattg cttttgtgaa
     2341 ttaattt
//
LOCUS       DBANUU010000318         2320 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000318 DBANUU010000000
VERSION     DBANUU010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.324"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..682
                     /locus_tag="ACIE68_004875"
     CDS             <1..682
                     /locus_tag="ACIE68_004875"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFX5911022.1"
                     /translation="TPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQAGNV
                     GRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRRR
                     GLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKL
                     NVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITD
                     FMTARGYH"
     gene            complement(731..2230)
                     /gene="pitB"
                     /locus_tag="ACIE68_004876"
     CDS             complement(731..2230)
                     /gene="pitB"
                     /locus_tag="ACIE68_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFX5911023.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT          615 a          596 c          569 g          540 t
ORIGIN      
        1 aacacctcca ttaattgccg ttattggtag cgatggttca ggcaagtcaa cggtgtgtga
       61 acatcttatt accgttgtcg aaaaatatgg tgctgccgaa agagttcatt taggaaaaca
      121 ggccggaaat gtcggtcgtg cagtgacaaa attaccgttg atgggaaaat ccttacataa
      181 aacaattgaa cgaaatcagg tgaaaacagc aaaaaaattg cctggaccag ttccggcgct
      241 ggtaattaca gcgtttgtcg cccgtcgctt actgcgcttt cgtcatatgc ttgcctgtcg
      301 tcgtcgcggg ttaattgttc taaccgaccg ttatcctcag gaccaaattc ctggcgctta
      361 cgatggtacg gtgttcccac ctaacgttga aggtggtcgt tttgtctcat ggctggcaag
      421 ccaggaacgt aaagcgtttc actggatggc gagccataag cctgatctgg tcatcaaact
      481 caatgttgac cttgaagttg cctgtgcacg taaacccgac cataaacggg aatcgctggc
      541 gaggaagatt gccataacgc cacagttaac ctttggtggt gcacaactgg ttgatatcga
      601 tgccaatcag ccactggaac aggtgttggt tgatgcagaa aaagcgatta cggattttat
      661 gaccgcgcgt ggttatcact agtcaaaaat ggaaatgccc gatcgccagg accgggcatt
      721 ttcaggaagg ttaaatcaac tgcaatgcta tccagtacag cccaccagaa agaaaaattg
      781 ccgccggtaa agtaaatacc cacgccatca ggatgctggt taccgtttta cgctgtaacc
      841 caccgccgtc caccaccatc gtccctgcaa ctgcagacga gaggacgtgt gttgtggaga
      901 cgggcatccc aatataactg gcaagaccga tagacactgc cgccgtcatt tgtgccgcca
      961 tgccttgcgc atacgtcatg ccgcgcttac caatcttctc accgatggtc atcgctacac
     1021 gacgccagcc aatcatggtg ccaatgccga gcgccagtgc taccgccatg atgatccaca
     1081 ccggagcgta ctcaatggtg cttaacatat cgctgcgcag ttttttcagc aggttctggt
     1141 cttctttact gacgcctggc agtttcgcca gcttcgcgga ggtatcagag atgcacagca
     1201 taatgcggcg cagctggctg cgctgactca cgcttaacgg ctcgtaactt tccatattgc
     1261 ctggcagcat cgttttaacg cgcgcaatcg catcaaaggt atttgccgga tgacagtgaa
     1321 actctgttac ttgcgtgcca tcagtcgatg ctgcaggcaa tggaggttcc atcgcaatca
     1381 acttctgcgg cagttcagga tgctgttgca ggtagtgttc gaagttggtg acggcatcgc
     1441 gggtacgggt aatttcatag ccggacgcgt tcatattgac gacgaagcca gcaggggcaa
     1501 tccccaccag taccagcatc accaggccga tccctttttg tccgtcgttc gcgccgtgcg
     1561 aaaacgccac gcccgcagcg gaaacaatca gcgcaatacg cgtccagaat ggcggtttac
     1621 gtttgccttt tttctttttg cgatcttccg gaatgcggtg aatacggtca cgctttttcg
     1681 tcccgctcca gtagcgtcgc agcaggaata tcaggcctcc cgcaatgacc aggccgacga
     1741 taggggaaac aatcagcgag gagaaaattt tggtcacttc acgcaggttt aacgcatcca
     1801 tcaccgatga gccggttaac agcgcgttgg ttaaaccgat gccgataatc gcaccaatca
     1861 aggtgtgcga actggaggcc ggtaaaccga agaaccacgt tcccaggttc cagataatcg
     1921 ccgccagcag catggaaaag accatcgcca ggccgtgggt tgaccccata ttcagcaaca
     1981 aatcggttgg caacatatgg acaatggcat aggcaacgct aagtccgccc aataacacgc
     2041 caaaaaagtt aaaaaatgcc gccatcacca cagcaagttg tggttgcatg gcacgagtat
     2101 aaataacggt tgccaccgca ttcgccgtgt catgaaaacc attgattgct tcgtagaaca
     2161 acacaaatgc cagagcaagc aataacaaaa gccctgtgta tatatcaagg ccaacaaata
     2221 aatttagcat atattagatt acgccatttt gaatttacga acggacgcat tatcagtgac
     2281 tttaacggca tgggcaaagt gaaatatcat tttttaacga
//
LOCUS       DBANUU010000319         2285 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000319 DBANUU010000000
VERSION     DBANUU010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.325"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..567)
                     /locus_tag="ACIE68_004877"
     CDS             complement(<1..567)
                     /locus_tag="ACIE68_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911024.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLL"
     gene            complement(662..868)
                     /locus_tag="ACIE68_004878"
     CDS             complement(662..868)
                     /locus_tag="ACIE68_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFX5911025.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1611..1778
                     /locus_tag="ACIE68_004879"
     CDS             1611..1778
                     /locus_tag="ACIE68_004879"
                     /inference="COORDINATES: protein motif:HMM:NF017162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911026.1"
                     /translation="MIFHTRYRLTPVDLEARNSAVAHRLTELTANFTSACRASVWSPE
                     RSVTEARAVIG"
     gene            complement(1992..2192)
                     /locus_tag="ACIE68_004880"
     CDS             complement(1992..2192)
                     /locus_tag="ACIE68_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911027.1"
                     /translation="MVIRKKKCWDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVYILCSW"
BASE COUNT          592 a          545 c          611 g          537 t
ORIGIN      
        1 caggagcacg tactgatcca tcagatccgt cagtgctgcg ctctgcttct gacggttgat
       61 gatccattcc tccagctctt tttctgattt ccgtttcaga ttctccgtcc agtcgtcaag
      121 gcagtttccg gtcattaaat caagccagca aagccagacc agtttgaggc gcaactccgt
      181 gccaccaaac tgacggaaga ggctgatcag tttatttgcc agagaacgct gcctcagacg
      241 ttcacattca gacaggagaa tgccttccag catcggatga tgaaccagca gatgttcact
      301 ggtgcataaa ttcactatca gatcatcatc caggggcggt gtggcccgtt tgtcagggcc
      361 ataaatcagc cggtgcaggc tctggggtac gatggcaatc tccggttttt caccattgaa
      421 tgacacccaa ccgggcgtca gattatggaa gtagtagata ttcggatgac acacgcccca
      481 ggatacggcg tgccgggtga agccggtcag ccatttctgg tagctttcgt ggagccattc
      541 gtagcgggtc tgatactgtt gtttcatttt catattcctc acggggatat aacaggcagc
      601 agaatgccgc cggcaggccg gagattacgt ctgctgcggg tgaacggata acggtcagaa
      661 attacggacg ggactgtgca atacgcgcct gcagccaggc ttccacttca cttttcagcc
      721 agcgggagct gcgccccatt ttgatggggg caggaaagcc cccatctttg atgagtttgt
      781 aaaaccactt atcggttaag ccggtaagtt gagtgataaa cgccatgtcg accatctggt
      841 catccatcag cgaaacaggg gtagccatga gaatgttcct ccggatgttg ttaaaccagc
      901 cggtgtggtc atgaaaacag cgcgccacct tcactggcat aacaaccggg gagaaacgta
      961 aaaaataatc gggaatgtgg ctgtccggag ataaacgggg ggattgcgat ggcagagccg
     1021 tagcggaatc ccggggggtt gttgcgaaaa gatgcttccg cacgcacgcg cgataaagga
     1081 gggaggactt tcgtgagaca aggggaaaac tagcgttctg ataattgcag agttgttttc
     1141 ggaggtggtt acagtgagtc agcccgttcc tggcgaggag gagactgata tcaccgatgg
     1201 gataaggctg tctgattggt attataaata taagaagtca tcagagagta acgtaaagga
     1261 tttgccagag taagctaaga gagtaaatct ctccggtaac agagcgctgt gaagcattct
     1321 gtatcagagt gcaaatacat gaacgctgat gcgtaaaatc agcagtgaca cgccagctcc
     1381 ccctgtgccc attcctgtat aagacatgaa ctgagcgtat taatgctcct gctctgagac
     1441 tgagccggtg agtgaggggc tgagaaatcc ctcttgacac agctctaaac cagaaatact
     1501 tccccggttg ttgcggtgcg tccggatccc cgataacgga acactaaggc tgtattgcgg
     1561 gacaccgggt aagtggcagc ctgtaatgtg ttctgtcaac gccagaaaaa atgatttttc
     1621 acacgcgtta tagactcact cctgtggacc tggaagcccg taactctgcg gttgcccacc
     1681 gactgacgga actgaccgca aactttacga gtgcgtgcag ggcttcagtc tggtcacctg
     1741 agcgttcggt aacggaggcg cgggcggtaa taggctgatt ttcaggtatt accggcttac
     1801 agcgtgtggt tgatattgag gggctgtgag tttcgcgtat ttgtcagaaa cccagacgag
     1861 ttttcctgta tgggacgaaa taaggcagcg gcaatacctg gaaaaattta atatggtatg
     1921 aaggagaacc tgtcggttac gcttaatata cccggtacac tgtcagctca ggggatacat
     1981 gaaccgttat ctcaccagct acacagtata tacacactga cgctgacagt agtcagcagg
     2041 accacaataa tcagtgccag cagtatggta acgatacggt ctgtattccg gtaatagccg
     2101 aaggggtcga cagaaccaca atacgggcaa cacacagtat tatgagtaat cgcgtttcca
     2161 cagtcccagc acttcttttt gcgtatcacc ataactccct cctcacagcc ttacccgtaa
     2221 ccggtttttt acattaaaaa aacaacgctg aaaattcaac cgtcagtccg gagacgatag
     2281 ttggg
//
LOCUS       DBANUU010000320         2276 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000320 DBANUU010000000
VERSION     DBANUU010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.326"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..1818
                     /locus_tag="ACIE68_004881"
     CDS             <1..1818
                     /locus_tag="ACIE68_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFX5911028.1"
                     /translation="NYYLPKESAYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARV
                     GYSKMLLGVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGK
                     KHRDNTLTMKRFSNGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFL
                     GDKRIEGSVWPKSIRGSTPKVRGTCQIERAASESLHFMRFHVACPHCGEEQYLKFGDK
                     ETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSS
                     GEEIEPPDSVTFHIWTAYNPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIG
                     ERPDAEVMAERKEHYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQII
                     MGRHDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRV
                     IPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVH
                     FPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISI
                     SRWQLDLSALLASLQEEDGAATNKKTLADYARALSGEDE"
     gene            1815..2021
                     /locus_tag="ACIE68_004882"
     CDS             1815..2021
                     /locus_tag="ACIE68_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFX5911029.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2018..>2276
                     /locus_tag="ACIE68_004883"
     CDS             2018..>2276
                     /locus_tag="ACIE68_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFX5911030.1"
                     /translation="MKTSTIPILLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFF"
BASE COUNT          551 a          575 c          676 g          474 t
ORIGIN      
        1 aattactatc tcccgaaaga atccgcatac caggaagggc gctgggaaac actgcccttt
       61 cagcgggcca tcatgaatgc gatgggcagc gactacatcc gtgaggtgaa tgtggtgaag
      121 tctgcccgtg tcggttattc caaaatgctg ctgggtgttt atgcctactt tatagagcat
      181 aagcagcgca acacccttat ctggttgccg acggatggtg atgccgagaa ctttatgaaa
      241 acccacgttg agccgaccat ccgcgatatt ccgtcgctgc tggcgctggc tccgtggtat
      301 ggcaaaaagc accgggataa cacgctcact atgaagcgtt tttccaatgg tcgtggcttc
      361 tggtgcctgg gcggtaaagc ggcaaaaaac taccgtgaaa agtcggtgga tgtggcgggt
      421 tatgatgaac ttgctgcctt tgatgaggat attgaacagg aaggctctcc gacgttcctt
      481 ggcgacaaac gtattgaagg ctcggtctgg ccaaagtcca tccgtggctc cacgcccaaa
      541 gtgagaggca cctgccagat tgagcgtgca gccagtgaat ccctgcattt tatgcgtttt
      601 catgttgcct gcccgcactg cggggaggag cagtatctta aatttggcga caaagagacg
      661 ccgtttggcc tcaaatggac gccggatgac ccctccagcg tgttttatct ctgcgagcat
      721 aatgcctgcg tcatccgcca gcaggagctg gactttactg atgcccgtta tatctgcgaa
      781 aagaccggga tctggacccg tgatggcatt ctctggtttt cgtcatccgg tgaagagatt
      841 gagccgccgg acagtgtgac ctttcacatc tggacggcgt acaacccgtt caccacctgg
      901 gtgcagattg tcaaagactg gatgaagacg aaaggggata cgggaaaacg taaaaccttc
      961 gtgaacacca cgctcggtga gacatgggaa gcgaaaatcg gcgaacgtcc ggatgctgag
     1021 gtgatggcgg agcggaaaga gcattattca gcgcccgttc ctgaccgtgt ggcttacctg
     1081 accgccggta tcgactccca gctggaccgc tacgaaatgc gcgtatgggg atgggggccg
     1141 ggtgaggaaa gctggctgat tgaccggcag attattatgg gccgccacga cgacgagcag
     1201 acgctgctgc gtgtggatga ggctatcaat aaaacctata cccgccggaa tggtgcagaa
     1261 atgtcggtat cccgtatctg ctgggatact ggcgggattg acccgaccat tgtgtatgaa
     1321 cgctcgaaaa agcatgggct gttccgggtg atccccatta aaggggcatc cgtctacgga
     1381 aagccggtgg ccagcatgcc acgtaagcga aacaaaaacg gggtttacct taccgaaatt
     1441 ggtacggata ccgcgaaaga gcagatttat aaccgcttca cactgacgcc ggaaggggat
     1501 gaaccgcttc ccggtgccgt tcacttcccg aataacccgg atatttttga tctgaccgaa
     1561 gcgcagcagc tgacagctga agagcaggtc gaaaaatggg tggatggcag gaaaaaaata
     1621 ctgtgggaca gcaaaaaacg acgcaatgag gcgctcgact gcttcgttta tgcgctggcg
     1681 gcgctgcgca tcagtatttc ccgctggcag ctggatctca gtgcactgct ggcgagcctg
     1741 caggaagagg atggtgcagc aaccaacaag aaaacactgg cagattacgc ccgtgcctta
     1801 tccggagagg atgaatgacg cgacaggaag aacttgccgc tgcccgtgcg gcactgcatg
     1861 acctgatgac aggaaaacgg gtggcaacgg tacagaaaga cggacggaga gtggagttta
     1921 cggccacttc cgtgtctgac ctgaaaaaat acattgcgga gctggaagtg cagaccggca
     1981 tgacacagcg acgcagggga cctgcaggat tttatgtatg aaaacgtcca ccattcccat
     2041 ccttctgggg ccggacggca tgacatcgct gcgcgaatat gccggttatc acggcggtgg
     2101 cagcggattt ggtgggcagt tgcgggcgtg gaacccaccg ggtgaaagtg tggatgcagc
     2161 cctgctgccc aactttaccc gtggcaatgc ccgcgcagac gatctggtac gcaataacgg
     2221 ctatgccgcc aacgccatcc agctgcatca ggatcatatc gtcgggtctt ttttcc
//
LOCUS       DBANUU010000321         2226 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000321 DBANUU010000000
VERSION     DBANUU010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.327"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            85..567
                     /locus_tag="ACIE68_004884"
                     /pseudo
     CDS             85..567
                     /locus_tag="ACIE68_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
     gene            564..740
                     /locus_tag="ACIE68_004885"
     CDS             564..740
                     /locus_tag="ACIE68_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFX5911031.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            743..1084
                     /locus_tag="ACIE68_004886"
     CDS             743..1084
                     /locus_tag="ACIE68_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein NinX family protein"
                     /protein_id="HFX5911032.1"
                     /translation="MDYSQLSDFEINRMVGDIIFKGLWACKPETSGNNTNKWYYGNAD
                     VSLEPLNPLPDYCNDPSASWPIIEKYRISILDQLTEWCVDAKGVSPIFDTRPLRAAMI
                     VFLLMQEANNA"
     gene            1205..1294
                     /locus_tag="ACIE68_004887"
                     /pseudo
     CDS             1205..1294
                     /locus_tag="ACIE68_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
     gene            1291..1653
                     /gene="rusA"
                     /locus_tag="ACIE68_004888"
     CDS             1291..1653
                     /gene="rusA"
                     /locus_tag="ACIE68_004888"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFX5911033.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1650..1790
                     /gene="ylcG"
                     /locus_tag="ACIE68_004889"
     CDS             1650..1790
                     /gene="ylcG"
                     /locus_tag="ACIE68_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFX5911034.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            1876..>2226
                     /gene="quuD"
                     /locus_tag="ACIE68_004890"
     CDS             1876..>2226
                     /gene="quuD"
                     /locus_tag="ACIE68_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFX5911035.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRL"
BASE COUNT          607 a          476 c          589 g          554 t
ORIGIN      
        1 ctctcctgaa tcaggagagc agattacccc ctggatctga tttcaggcgt tgggtgtgga
       61 tcactattgc accgttcgtg acagcaaacg ccgctttggc tttttgatgc actggatatt
      121 gagtttggat tctggctgga ttcggcagcg agcgacaaaa atgctctgtg cgctcactgg
      181 ttaactgagg ccgacgacgc gctaaattct gagtggataa gccacggtgc aatctggaat
      241 aacccaccgt acagcaatat caggccgtgg gtggaaaaag ccgctgagca gtgcatacaa
      301 cagcgacaga cggtagtgat gcttgtgcca gaggatatgt ctgtcggatg gttcagcaag
      361 gctctggaga gtattgacga agttcgcatc atcactgatg gacggattaa ttttatcgaa
      421 ccatcgacag ggctggagaa gaagggaaac agcaaaggtt ccatgctgct gatttggcga
      481 ccgttcatca gtcctcgacg gatgtttact accgtatcca aagcggcatt gatggcgatc
      541 gggcagggcg tcaggagggc tgcatgagac gacagcggcg aagtttcacc gacatcatct
      601 gcgaaaactg caaatacctt ccaacgaaac gctccagaaa taaacgcaag ccaatcccaa
      661 aagaatctga cgtaaaaacc ttcaactaca cggctcacct gtgggatatc cggtggctaa
      721 gacatcgtgc gaggaaatga caatggatta ttcacagtta agtgattttg aaattaaccg
      781 aatggtagga gacataattt ttaaaggcct ttgggcatgt aagcctgaaa catcagggaa
      841 taacaccaac aaatggtatt acggaaatgc cgatgtaagt cttgagccat taaacccttt
      901 gcctgactac tgcaatgatc cgagcgcttc atggccgatt attgagaaat acaggatttc
      961 tatcttagac cagttaactg aatggtgtgt ggatgcaaaa ggcgtaagcc caatatttga
     1021 taccagacct ctccgcgccg ccatgattgt ctttctcctg atgcaggagg ccaataatgc
     1081 ttagcccatc tcaatccctt caatacctga aaggaagcat agagcgggct tcaatgtgca
     1141 cagagtggat tctatctagg tttagcgcat acagaagatt gccggtaaag ggcatgccaa
     1201 gcaagtcgat gctgcatatg caaaagaatg cgcgctggaa ggtatggcga gaacacaggt
     1261 tatctggctg aaagaggggg ttattaaggc gtgaatacct acagcatcac attaccctgg
     1321 cctccgagca ataatcgcta ttaccgccat aatcgcgggc gcacgcacgt cagcgcagag
     1381 gggcaggcat accgcgataa cgtcgcccga atcattaaaa acgcaatgct ggatatcggc
     1441 ctggctatgc ctgtgaaaat ccgcattgag tgccacatgc cggatcgccg tcgccgtgac
     1501 ctggataatc tgcaaaaagc cgcttttgac gcactcacta aagcaggttt ctggctggat
     1561 gatgctcagg tcgttgatta ccgcgttgtg aagatgcctg ttaccaaagg tgggaggctg
     1621 gaactgacca tcaccgaaat ggggaatgaa tgatgtttga gtttaatatg gcagaacttc
     1681 ttcgccaccg ctgggggcgt ctgcgcttat atcgtttccc cggttctgtt ttgaccgatt
     1741 accgaatact gaagaattac gccaaaaccc tgacaggagc aggagtatga agtcagagat
     1801 aacaatcaac taatactgtt ttgttgattt ttgcttgtaa ttggcgttct ggtctgattt
     1861 ttgtggagta agttgatgcg tgatattcag atggttcttg agcgttgggg agcgtgggcg
     1921 gctaataatc atgaagatgt gacctggtcg tccattgccg ccggttttaa gggattaatt
     1981 acttcaaaag taaaatctcg cccgcaatgt tgtgacgatg acgcgatgat tatttgcggg
     2041 tgcatggccc gtctgaaaaa gaacaacagc gatttgcacg atttattagt agattattat
     2101 gtagtcggta tgacattcat gtcactggca ggtaagcatt gctgctctga tggttatatc
     2161 gggaaaaggt tacagaaggc tgagggcata attgaaggga tgttaatggc attagatatc
     2221 cggtta
//
LOCUS       DBANUU010000322         2210 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000322 DBANUU010000000
VERSION     DBANUU010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.328"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            86..418
                     /gene="hdeA"
                     /locus_tag="ACIE68_004891"
     CDS             86..418
                     /gene="hdeA"
                     /locus_tag="ACIE68_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFX5911036.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            534..860
                     /gene="hdeB"
                     /locus_tag="ACIE68_004892"
     CDS             534..860
                     /gene="hdeB"
                     /locus_tag="ACIE68_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFX5911037.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            924..1571
                     /locus_tag="ACIE68_004893"
     CDS             924..1571
                     /locus_tag="ACIE68_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFX5911038.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1613..2143)
                     /locus_tag="ACIE68_004894"
     CDS             complement(1613..2143)
                     /locus_tag="ACIE68_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFX5911039.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
BASE COUNT          658 a          429 c          497 g          626 t
ORIGIN      
        1 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
       61 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
      121 gcttcttctg ccagttgtaa gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
      181 accggtcaac tcctggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
      241 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
      301 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
      361 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
      421 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
      481 aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac aaaatgaata
      541 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
      601 acgcacaatc tgcgctggca gccaatgaat ccgctaaaga tatgacctgc caggaattta
      661 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
      721 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
      781 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
      841 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
      901 gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt atactcgcgg
      961 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
     1021 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
     1081 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
     1141 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
     1201 taggtctgac gacggcagcg gatatctggg tgaccgccgc cataggtatg gttattggca
     1261 gcggtatgta cgaactgggt atttatggtt cagtgatgac cttgttggtg ctggaagtct
     1321 tccatcaatt aaccttccgc ctgatgaaca aaaattacca tttacagctg acattagtga
     1381 acggtaatac ggtatcaatg ctcgactggt ttaagcagca aaaaataaaa acggatttag
     1441 tttcgttgca ggaaaatgaa gatcatgaag tggtcgccat tgatatccag cttcatgcca
     1501 caacgtcgat agaagacctg ttgcgattat taaaaggtat ggcaggggtg aaaggcgttt
     1561 caattagcta atctgtgtgg cagcgtagcc agactcaccg taagcctgaa attcacacca
     1621 gataatcaat atgctgatgg cgtaccagct cattgatccg cttaagattg aggatcatca
     1681 tgatattgtg tttatggcag tagaacgttt ttagcgatat tttcagtttc tccagaatat
     1741 cttccgttgt catttgattc gcaatgcagc tcaaaacctg atgttgcttg cgcgaaagtg
     1801 acacattcgc ttcaggcaca gagcagtaac tgccattgat gagtgcgaca aaatcaatga
     1861 tagtgatagt ccgcggaaca aaggtcaccg gtactttccc cagacagcgt ttaatattaa
     1921 agggggagaa aataatcaca tggacaggtt ttaatcgttc aatttgagtg agaaaattag
     1981 aatgaaatac attattcatt aatgtatcaa taatgacgaa ggcattctga tgcaacatta
     2041 cgtcgatctc ttcttcgttc tgtatatgaa cgacattacc tttactcaga atgtttttca
     2101 tcgcggtgaa gaacatcgta tccctggtaa ttataagaaa cataaccgac tcctggtgcg
     2161 gacattaact ttataatcag tttgccaaag gtaattaaca ggacagggct
//
LOCUS       DBANUU010000323         2177 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000323 DBANUU010000000
VERSION     DBANUU010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.329"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            13..1767
                     /locus_tag="ACIE68_004895"
     CDS             13..1767
                     /locus_tag="ACIE68_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HFX5911040.1"
                     /translation="MRNKNIIMLLMSSLILSGCGPESENKESQQQQLSTPTDQQVLAA
                     QQAAIKEAEQRSVADKATADAKAKALAQQEAQQYSDKQALQGRLQAAPKYQHAAREKA
                     ASQIANPGTARYKQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNHGLLPPPDAV
                     RVEEIVNYFPYDWDIKDKQSIPATKPIPFAMRYELAPAPWNEQLTLLKIDILAKDHKS
                     EELPASNLVFLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALP
                     SISGSHKAEINAAIDSLDAEGSTNGGAGLELAYQQAAKGFIKGGINRILLATDGDFNV
                     GIDDPKSIESMVKKQRESGVTLSTFGVGNSNYNEAMMVRIADVGNGNYSYIDTLSEAQ
                     KVLNSEMRQTLITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIG
                     AGKHITLLFELTLNGQKASIDKLRYAPDNKSVKSDKTKELAWLKIRWKYPQGKESQLV
                     EFPLGPTINAPSEDMRFCAAVAAYGQKLRGSEYLNNTSWQQIKQWAQQAKGEDPQGYR
                     AEFIRLIELADGVTDISQ"
     gene            complement(1838..>2177)
                     /locus_tag="ACIE68_004896"
     CDS             complement(1838..>2177)
                     /locus_tag="ACIE68_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
                     /protein_id="HFX5911041.1"
                     /translation="HLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRESNSDSA
                     PYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLEIKHSQP
                     KNRASGKRVS"
BASE COUNT          633 a          465 c          524 g          555 t
ORIGIN      
        1 aaaggatttt atatgcgaaa taaaaatata atcatgttgc ttatgagtag tttgattttg
       61 tcaggatgcg ggccggaatc tgagaataag gaaagtcagc aacaacagct cagtaccccc
      121 acagaccagc aagtgcttgc cgcgcaacaa gctgcaataa aagaggccga gcaaagatcc
      181 gtcgccgata aagccactgc agacgcgaaa gcaaaagcct tagcccagca agaagcgcaa
      241 caatattcag acaaacaggc tttgcagggg cgcttacagg cggctccaaa atatcaacat
      301 gcagccagag aaaaagcagc gtcccaaatc gcaaatccag gaaccgctcg ctacaagcag
      361 ttcgatgata atccggttaa gcaggtagcg caaaacccgt tggcgacgtt tagccttgac
      421 gtcgacaccg gcagttatgc gaatgtacgc cgttttctca atcatgggct gttacctccg
      481 ccagacgctg tgcgggtgga ggagatagtc aattatttcc cgtatgactg ggatatcaaa
      541 gacaaacaat ctattccggc cactaagcca atacctttcg ctatgcgcta cgaactggca
      601 cctgcgccgt ggaatgaaca gctaacattg ctgaaaattg atatcctggc gaaagatcat
      661 aaaagtgaag agttaccagc ttctaatctg gtctttctta tcgacacttc tggttcaatg
      721 atttctgatg aacgtttgcc acttatccag tcttcgttga aattattggt caaagaactt
      781 cgtgagcagg ataacattgc catcgtgacc tacgctggcg actcccgtat tgcgttgcct
      841 tctatctccg gtagtcacaa ggcggaaatt aatgccgcaa ttgattcgct ggatgccgaa
      901 ggcagtacca atggcggtgc cgggctggaa ctggcttatc agcaggcggc gaaggggttt
      961 attaagggcg gcatcaatcg cattttatta gccactgacg gtgactttaa cgttggcatt
     1021 gacgatccaa aatcgattga atcaatggtc aaaaaacagc gggagtctgg tgttactctg
     1081 tcgacgtttg gcgtggggaa tagcaattac aacgaggcaa tgatggtgcg aattgccgat
     1141 gttggtaacg gcaactacag ctacattgat accctctctg aagcgcagaa agtattgaat
     1201 agtgaaatgc ggcagacgtt gattaccgta gcaaaagatg tcaaagcgca aattgagttt
     1261 aaccccgcgt gggtaacgga ataccgtcag attggttatg aaaagcgcca acttcgggcg
     1321 gaagatttta ataacgacaa cgttgatgcg ggggatattg gcgcaggcaa acatataacg
     1381 ttgttattcg aattaacgct gaacgggcaa aaagcatcaa ttgataagtt acgctatgcc
     1441 ccggataaca aatcagtgaa atcggacaaa acgaaagaac tggcctggtt aaagattcgc
     1501 tggaaatacc cgcagggaaa agaaagtcag ttagttgaat tcccgctggg gccaacaata
     1561 aacgccccct ctgaagatat gcgtttttgc gcagcagtag ctgcatatgg gcaaaagtta
     1621 cgcggttctg aatacctgaa caatacctcc tggcagcaga tcaaacagtg ggctcagcag
     1681 gcaaaagggg aagatccaca gggttacagg gcggaattta ttcgcctgat tgaactggcg
     1741 gatggtgtga ctgacatcag tcagtgatga ctgtttagca aactatgttc gaccagtcag
     1801 catatttgct gactggtcga attgattaac aatgatgtta actcactctt ttgccggatg
     1861 ctctattttt gggttgtgag tgttttattt ctaattgtgt ataagaagca ttgattcgtg
     1921 catcgccata tttatctaaa taacaatttc tgtttaccag tcgatgttga aaatcaattt
     1981 cgtactgctc ttctgctgaa tattttttaa atctgtcagc atagtttttt aataacgtat
     2041 agggggcgct gtcagaattg ctttcccttt gttcaatcac ttcctgggct gccatgcaca
     2101 caaacgcacc gcaactttgg cttaagtatt gctgaagatc ggcatgtaaa aatattatct
     2161 ctggttcatt taaatga
//
LOCUS       DBANUU010000324         2141 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000324 DBANUU010000000
VERSION     DBANUU010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.330"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..567)
                     /locus_tag="ACIE68_004897"
     CDS             complement(<1..567)
                     /locus_tag="ACIE68_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911042.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLL"
     gene            complement(654..860)
                     /locus_tag="ACIE68_004898"
     CDS             complement(654..860)
                     /locus_tag="ACIE68_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFX5911043.1"
                     /translation="MLTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1619..1768
                     /locus_tag="ACIE68_004899"
     CDS             1619..1768
                     /locus_tag="ACIE68_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFX5911044.1"
                     /translation="MEKVISHTRYKLTSAELEAFNSVVDHWLAELTMNKLYDRVPASV
                     WKYVI"
BASE COUNT          559 a          466 c          594 g          522 t
ORIGIN      
        1 caggagcacg tactgatcca tcagatccgt cagtgctgcg ctctgcttct gacggttgat
       61 gatccattcc tccagctctt tttctgattt ccgtttcaga ttctccgtcc agtcgtcaag
      121 gcagtttccg gtcattaaat caagccagca aagccagacc agtttgaggc gcaactccgt
      181 gccaccaaac tgacggaaga ggctgatcag tttatttgcc agcgaacgct gcctcagacg
      241 ttcacattca gacaggagaa tgccttccag catcggatga tgaaccagca gatgttcact
      301 ggtgcataaa ttcactatca gatcatcatc caggggcggc gtggtccgtt tgtccggacc
      361 ataaatcagc cggtgcagac tctggggtac gatggcaatc tccggttttt caccattgaa
      421 tgacacccac ccgggcgtca gattatggaa gtagtagata ttcggatgac acacgcccca
      481 ggatacggcg tgccgggtga agccggtcag ccatttctgg tagctttcgt ggagcagctc
      541 gtaacgggtc tgatactgtg gtttcatttt catattcctc acgggggata acaggcagca
      601 gaacgccgcc ggcaggccgg agactgcgtc tgttgcagat gaacggttaa gaattacgga
      661 cgggactgtg caatacgcgc ctgcagccag gcttccactt cgcttttcag ccatcgggag
      721 ctgcggccca ttttgatggg cgcaggaaag tcaccatcct tgatgagttt gtaaaaccac
      781 ttatcggtca ggccggtcag ttgagtgata aatgccatgt cgaccatctg gtcatccatc
      841 agtgaaactg gggtgagcat gagaatgttc ctccggatgt tgttaaacca gccggtgtgg
      901 tcatgaaaac aacgcaccac cttcaccggc ataacaaccg gggagaaacg taaaaaataa
      961 tcgggaatgt gaccgccagg agataaacgg ggaggttacg gtggctgagc cgtaccggta
     1021 tgccgggtgg ttgttacgaa acgatgcttc cgcacgcgtg cgcgataaag aagggatgat
     1081 tttcgtgagg aagaggggaa aactggcgtt ctgataattg cagagttatt tacggggcgg
     1141 ttacgatggg gcagaccgtg tccggtgggc aggcgacagg tatcaccgat gggataagac
     1201 tgtctgattg gtattataaa tatataagag agatcaaaca catgagatca aatacatgag
     1261 ttcatatatg tgaaataaac acggtgagtg tgatcagtgg gcatgatgag aacgcagtag
     1321 caacagactg cgctatccct gatagctggc gtataacccg cgtatataaa acgtatctgc
     1381 aatgtacgtg aacagagtgc attaagcatc cgtctgagcg gtaaacatat ccgacctggt
     1441 gagtgacagt ctgagagacc ggttaagtgg tggctcggga gtaatgagtt tggggtgggt
     1501 accagaatgc cagtctggcg gtatgctgac agtatcacgt taattgcagc ataaagtgat
     1561 gagaggctaa tggaagttaa aaccaaagca gttccgtcgt tgttcatccc gggagacgct
     1621 ggaaaaagtg atttcccaca cgcgttataa acttacctct gcggaactgg aagccttcaa
     1681 ctctgtggtt gatcactggc tggcagaact gacaatgaac aagctttacg atcgtgttcc
     1741 cgcttcagtc tggaaatatg tcatctgagc gttcggtaat cacaactgca tcgcgccagg
     1801 tgttatccgc tttgtgattc ttattggctc ttctcaggtt attaaaagct gaatatgttc
     1861 ctgttgtcgg gaaccaggat aataccggga acgttaagcg gtatagcgac aacaaagaat
     1921 tcgaaatcaa aagggaatgg tgccggaggc gtgggcggta atgggctgat tttcaggtat
     1981 taccggctta cagcgtgtgg ttgatattga ggggctgtga gtttcacgta tttgtcagaa
     2041 acccagacga gttttcctgt atgggacgaa ataaggcagc ggcaatacat ggaaaaattt
     2101 aatatggtat gaaggagaac ctgtcggtta cgcttaatat a
//
LOCUS       DBANUU010000325         2094 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000325 DBANUU010000000
VERSION     DBANUU010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.331"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1336)
                     /locus_tag="ACIE68_004900"
     CDS             complement(<1..1336)
                     /locus_tag="ACIE68_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFX5911045.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYAQSWREAEPDNEDAPYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALMLQWCRQHRVDYYPLLVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQACQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDD"
     gene            complement(1333..2040)
                     /locus_tag="ACIE68_004901"
     CDS             complement(1333..2040)
                     /locus_tag="ACIE68_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFX5911046.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
BASE COUNT          568 a          506 c          482 g          538 t
ORIGIN      
        1 catcgtcaac gggcattggc tgcggttgcg gctccggttc aacagtttgc ttctcggtaa
       61 aattcgccgc ctgaattaat aatcgacgaa taattaatgc ttccagtgga caacgatttt
      121 ctgcatcaag gcggctcaca atcggcatta atgattcttc tgtaggttgg caggcctgca
      181 ctaattgttt ggcaactaga tataatggcc ataagggatg ttctgtatca cgcgtgagtg
      241 agatccgcgg cttacgtcct ttgtgcaaaa ccatctcgac cagtgaacgc cccagcgcag
      301 agagtttttc atcgctgagt atttcgtata aatgaaaacg cgtacgggcg ctgtcgccgg
      361 ggacataaag tagcggtttt ccctgatcgt taaccagatc acgcgcttca atcatcatca
      421 ccagtaatgg gtaataatcg acccgatgct gacggcacca ttgcaacatt aaagcatccg
      481 cctgggtgga gggatactcg cgccagtagt cacaaagttc tgccaacagg ctttcgcctt
      541 ctccacaata gacgcgttgc gcgtattcat aataaggcgc atcttcatta tcaggttcag
      601 cttcccgcca tgactgggcg taatccagaa gttcggggat tcccaagcgg cttgcgctat
      661 accagcggag taaatttctg tgcagttttg catcatcggg gatgatcact gcgcgatgtt
      721 gcgctaaata caatgtcagc cattctggat gcgatgacca aatccgagcg aacatttggt
      781 aaaaatcgat ggtgtcattt tgcacggcaa cgggcagatg cagaatggtc tggaagttaa
      841 aaacattccc cttcgcaatg gcatataaaa aggattccag ttcttcttcg tctatttccc
      901 cgacgttttc ctcctgcaac caccgcaatc tttctgctaa taacaccacg cactcgaagg
      961 ataaatgggc agtgttcatg gcgattgtgc acagcgacca gcgtaattca tcaatctctt
     1021 ccatcgagca ataatttaat tgctgaatga atcgctgcca ggcggagggc agaaaacgtt
     1081 cgctatcaga ggcaagtaac gcgcgaaagg cgagcagaat ttcatgttct gctacctcat
     1141 attcttctgg ttgccaaaca gatttagcag gcgactgcgc aatccggagc gcctcctcat
     1201 acgcctgacg aagttgttta aaaccctgcg gatcggtttc tggatgaaag gacggtaaca
     1261 gcgccagata agcgcggcgg ataatatcga cgtcagtcgt ttcctctata tcgaggatct
     1321 tccagcaatt tttcataggt cgctcatatc cttaagcgtt tccgggcatg cagaatagta
     1381 agccaatggt tgccaggaca aggtagggta atacgcatca tcactggcaa ataattgata
     1441 aaacatatca ttgccggaat cgtcgtattc tttacgcgct aagaattcgc gtaataaagg
     1501 cagatgatca tcttcgggag actgccaata ttgccgacca aaggtataac cggcaaagta
     1561 ttgccgccag ttactataat aacgtagagc tcgtagatga atgcgcgact ggatccatag
     1621 actttcttct tcgctcaacc agttattaag tacgcccatt cggcttaaaa aacccatccg
     1681 gacataatcc caggctttga ttcccccttc gccacagcat tcggcggtgc tggcgacaaa
     1741 ttgcgcataa gcctgacctt gctcatttag ttcagcaaga tagtcacgcc attcacatgg
     1801 cgaataacga aaccagcggt gataaaaccc tgccaaccga gcggcatgac cgttatcgat
     1861 cattcggtga attgtgcgca gcaggtcatc acggtcatca attccccatg aggaagaaag
     1921 gtcaaaaaag catgtaccta aatacatttt gggtgatgaa tgcgaaggta aacagcattc
     1981 attgcgtatt tccatcggag tggaaagtgc aaacaatatt ttttgcgact ccatgtccat
     2041 ataatttatc cttaaatatt cattaaatga atattagccg cattgcgagg ctcg
//
LOCUS       DBANUU010000326         2078 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000326 DBANUU010000000
VERSION     DBANUU010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.332"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1193)
                     /locus_tag="ACIE68_004902"
     CDS             complement(<1..1193)
                     /locus_tag="ACIE68_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFX5911047.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VMSVPALAADIVVRPGETVNGGTLANHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQ
                     WVQDGGTANKTTVTSGGLQRVNPGGSVSDTVISAGGGQSLQGRAVNTTLNGGEQWMHE
                     GAIATGTVINDKGWQVVKPGTVATDTVVNTGAEGGPDAENGDTGQFVRGDAVRTTINK
                     NGRQIVRTEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQ
                     IVKNGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSG
                     AAVSGT"
     gene            complement(1521..>2078)
                     /locus_tag="ACIE68_004903"
     CDS             complement(1521..>2078)
                     /locus_tag="ACIE68_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFX5911048.1"
                     /translation="ALYRDILPELDLVLWLIKADDRALSVDEYFWRQILQCGHQQVLF
                     VVTQADKTEPCHEWDMAGIQPSPAQEQNIREKTDAVFRLFRPVHPVVAVSARTGWELD
                     TLVSALMTALPDHAASPLMTRLQDGLRTESVRSQAREQFTGAVDRIFDTAESVCVASV
                     VRTALRAVRDTVVSVARAVWNWIFF"
BASE COUNT          436 a          615 c          540 g          487 t
ORIGIN      
        1 gtaccactga cagcggcacc ggaatcggcc agcagtacac cgccatttcc catggatacg
       61 gtggtggcgg taccaccgtt gcggacatcc agcgttccgc catcatccac gcgggtatta
      121 gtggctgtgt gtccggtcag cacatccagg cgtccgccat tttccagtac gacattatca
      181 gctttaccct ccacaacaga gaatgctccc aggcggttta tgccggtaac ggttgcagcc
      241 gtactggtaa ccagtgcgcc gccctgcttc agggtgacat tcgtggctgt accaccggcg
      301 tccacctgca gtctgccctt ctgattaacg gtggtattcc cggccacacc cccgtttttg
      361 acaatctgcc agccgtcact gttcacaaca gtgtcagacg ctgtaccgcc gttgtgcaca
      421 tactggtatc ccccattcag cgtggtatcc agtgcgtgac catgtacagt ctggtcgccg
      481 ccggcataaa ccacagtggt atttgccgtt ccttcagttc tcacaatctg gcgaccgttt
      541 ttattgatgg ttgtgcgtac ggcatccccg cgaacaaact gcccggtatc accgttttct
      601 gcatccggtc ccccttccgc cccggtatta acaacggtat ccgttgccac tgtaccgggc
      661 ttgacgacct gccagccctt atcattaatg acggttcctg tggctatcgc cccctcatgc
      721 atccactgtt cgccaccatt cagcgtggtg ttcactgccc gtccctgaag gctctgtccg
      781 cctccggcac tgataaccgt gtctgagaca cttccaccgg ggttcactct ctgaagacca
      841 ccactggtga cagtcgtttt gttggctgtt ccgccatcct gtacccattg cccgccggta
      901 ttggcctcgt tatccggccc atactccagc ccggtactga tggtcattcc gttggtcgta
      961 ccgaagacaa tctggttgtc atgatttgcc agtgttccgc cgttcacggt ttctcccggg
     1021 cgtacaacga tgtcagcagc cagtgccggg actgacatga cagcagcaag agacagagca
     1081 accgccacac cggcgcgttt tccccgtgag cgggccagtt cggaagccac caccagggtg
     1141 cccgtaatgt gattccatac cagcctgtag ctggtgttca gatgtcgttt catcagcttt
     1201 tccttagatt gagggtgaat aaaaaagccg gtacccacaa acgtggatac cggcaggtaa
     1261 agggttacag tgccggaggt ccggctgtgg gagtttctga attgtggttc tgttgttccc
     1321 tgaatgtctt tctgtacgta tctgtgcatg ttactgacaa aggttattat tagcttatcg
     1381 atcgatataa acgatcgata aaatagatat cgatcgtttt attctattat tgggctattg
     1441 cacggacaag agagacagta acgaaaacag cgcttttgtg agcagactca gagtcaggga
     1501 gggcatcagt cacgacaggt tcagaagaaa atccagttcc ataccgcacg ggcaacagag
     1561 accaccgtgt cacgaacagc gcgcagggcc gtacggacaa cagaggcaac acagacgctc
     1621 tccgctgtgt caaatatccg gtccaccgca ccggtaaact gttcacgggc ctgcgagcgg
     1681 acagactccg tgcgcagtcc gtcctgcagt cgggtcatca ggggactggc ggcatggtcg
     1741 ggaagtgctg tcatgagcgc actgaccagc gtatccagtt cccagccggt gcgggccgat
     1801 acggccacaa ccggatgtac gggccggaac agccggaata ccgcatctgt tttctcacga
     1861 atgttctgtt cctgtgcggg agaaggctga atacctgcca tatcccattc atggcagggt
     1921 tccgttttgt cggcctgcgt caccacaaac agcacctgct gatgtccgca ctgcaggatc
     1981 tgtcgccaga aatactcatc cacagacagg gcacggtcat cggctttaat cagccacagt
     2041 accaggtcca gttcaggcag aatgtcacgg tacagggc
//
LOCUS       DBANUU010000327         2034 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000327 DBANUU010000000
VERSION     DBANUU010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.333"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..1449
                     /locus_tag="ACIE68_004904"
     CDS             <1..1449
                     /locus_tag="ACIE68_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFX5911049.1"
                     /translation="DSALSMWPDNRIARDAHYLYRYDRHGRLTEKTDLIPEGVIRTDD
                     ERTHRYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLS
                     RKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGEQAKTQRRSLADT
                     LQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQMDP
                     VYTPARKIHLYHCDHRGLPLALVSTEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPG
                     QQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKY
                     GHLNNGGYGARPNKPPTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDY
                     TLYCRKWVKPNLTCTPQDDSQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPD
                     INKPDGPATAGIDDLGEILAKMKQRTSRGIRK"
     gene            1446..2015
                     /gene="ybfC"
                     /locus_tag="ACIE68_004905"
     CDS             1446..2015
                     /gene="ybfC"
                     /locus_tag="ACIE68_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFX5911050.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIKIQHELIRSEEK"
BASE COUNT          592 a          476 c          531 g          435 t
ORIGIN      
        1 gacagcgccc tcagcatgtg gccggataac cgtatcgccc gtgacgcgca ctatctttac
       61 cggtatgacc gtcacggcag gctgacggag aaaaccgacc tcatcccgga aggggttatc
      121 cgcacggatg atgagcgcac ccaccggtac cattacgaca gtcagcaccg gctggtgcac
      181 tacacgcgga cacaatatgc agagccgctg gtcgaaagcc gctatcttta cgacccgctg
      241 ggccgcaggg tggcaaaacg ggtgtggcga cgtgaacggg acctgacggg ctggatgtcg
      301 ctgtcacgga aaccgcaagt gacctggtac ggctgggacg gcgaccgcct gaccacaata
      361 cagaacgaca gaacccgcat ccagacgatt tatcagccgg ggagcttcac gccactcatc
      421 agggttgaaa ccgccaccgg tgagcaggcg aaaacgcagc gccgcagcct ggcggatacc
      481 cttcagcagt ccggcggcga agacggtggc agtgtggtgt tcccgccggt gctggtgcag
      541 atgctcgacc ggctggaaag tgaaatcctg gctgaccggg tgagtgagga aagccgccgc
      601 tggctggcat cgtgcggcct gacggtggag cagatgcaaa accagatgga cccggtgtac
      661 acgccggcgc gaaaaatcca cctgtaccac tgcgaccatc gcggcctgcc gctggcgctt
      721 gtcagcacgg aaggggcaac agaatggtgc gcagaatacg atgaatgggg caacctgctg
      781 aatgaagaga acccgcatca gctgcagcag cttatccgcc tgccggggca gcagtatgat
      841 gaggagtccg gcctgtatta caaccgccac cgctattatg acccgctgca ggggaggtat
      901 atcactcagg atccgattgg gctgaagggg ggatggaatt tttatcagta tccgctgaat
      961 ccggttcagt atatagattc aatgggactg gcatcaaaat atggacactt aaataatggc
     1021 ggatatggag cgagacccaa caaaccgcct acgcccgatc caagtaaatt gccggacata
     1081 gcgaaacaat taagactgcc atatcctatt gaccaggcca gtagtgcgcc taatgttttc
     1141 aaaacattct tcagagcatt aagcccttac gactacacac tgtattgcag gaagtgggta
     1201 aaaccaaatc tgacttgtac gccacaggat gattcccagt atccagggat ggatacaaag
     1261 acagcaagtg attacctgcc acagacaaat tggccaacaa ctcaattacc accaggatat
     1321 acttgtgcag aaccctattt attcccagac attaataaac ccgatgggcc agcaacagca
     1381 gggatagatg atttgggtga aattttagct aaaatgaaac agagaacatc gagaggaata
     1441 agaaaatgaa aagagttttg ttctttttgc tgatgatatt tgttagtttt ggtgttatag
     1501 ctgattgcga aatacaagct aaagatcatg attgttttac tattttcgct aagggtacta
     1561 ttttttccgc gtttcctgtc ttaaataata aagccatgtg gagatggtat cagaatgagg
     1621 acatcggtga gtattattgg caaacagaac tgggtacatg taaaaataat aaattcacac
     1681 caagcggggc gagactctta attcgcgtcg ggtcactacg ccttaatgaa aatcatgcca
     1741 tcaaaggaac gttgcaggag ttgataaata cagcagaaaa aacggcgttt ctcggcgatc
     1801 ggtttagaag ttatataaga gcgggtatct atcaaaaaaa gagcagtgat ccagttcagt
     1861 tgttggcagt ccttgataat tcaatcatgg taaaatattt taaagatgag aaaccaactt
     1921 atgctcgaat gacagcacat ctcccgaaca aaaatgagtc ttacgaatgt ttaataaaga
     1981 tacagcatga actaattcgt agtgaagaga aataaaatct cttcctgaga aaac
//
LOCUS       DBANUU010000328         1999 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000328 DBANUU010000000
VERSION     DBANUU010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.334"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            8..1201
                     /locus_tag="ACIE68_004906"
     CDS             8..1201
                     /locus_tag="ACIE68_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5911051.1"
                     /translation="MSSKIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISLTGSKTVVLWALGGYGVSFTLLGLGSVLM
                     ATGAVTTAVGLGILIIARIVYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLVIHPLAPVWMLMAAPVWAVVMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAMLLAAAMSMMQLGLSPALTRQFDTDTTTISQQVAWLLGLSAIAALIAQFVV
                     LRPQRLTPVALLLSAGVLMSSGLAIMLTEQLWLFYLGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLAASHTLGYGLCALLVPLASKTGVSIALIVTALFAAVLFTIVSACIWH
                     YRTIK"
     gene            complement(1581..1961)
                     /gene="crcB"
                     /locus_tag="ACIE68_004907"
     CDS             complement(1581..1961)
                     /gene="crcB"
                     /locus_tag="ACIE68_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFX5911052.1"
                     /translation="MIKSLFAVIIGGSVGCTLRWLLSTRFNSLFPNLPPGTLVVNLLA
                     GLIIGTALAYFLRQPHLDPFWKLMITTGLCGGLSTFSTFSVEVFALLQAGNYIWALTS
                     VLVHVIGSLIMTALGFFIITILFA"
BASE COUNT          476 a          483 c          536 g          504 t
ORIGIN      
        1 cattacaatg tcatctaaga tcgaagatac gccccaaaaa accctgtcct gctggccact
       61 ggcgttcagt gccggtcttc tcggtatcgg acagaacggt ctgctggttg tactccctgt
      121 tctggtcata cagacaaatc tgagtctgtc tgtatgggct gccctgctga tgctgggctc
      181 aatgctgttt ctgccatctt ccccatggtg gggaaagcaa atttccctta ctggcagtaa
      241 gactgtggtg ctgtgggctc tgggaggata tggcgtaagc tttaccctgc ttgggctggg
      301 aagcgtgctg atggctaccg gtgccgtaac aacagcggtg gggttgggaa tattaatcat
      361 cgcccggatc gtctacggtc tgaccgtgtc agcaatggtg ccagcctgtc aggtctgggc
      421 attgcagaga gcgggagaag ggaatcgcat ggccgctctg gcaaccatca gctccggcct
      481 gagctgcggc aggctattcg ggccgctgtg cgcggcggca atgttggtca ttcaccctct
      541 ggcgccagtg tggatgctga tggcagctcc ggtgtgggct gtggtgatgc ttctgcggtt
      601 gcccggcaca ccaccacagc ccacaccgga gcgcaagagc gtcagcctga agcgggattg
      661 cctgccttat ctgctttgcg caatgttact ggctgcggca atgagcatga tgcagcttgg
      721 actttcgcca gcccttactc gccagttcga tactgatacc accactatta gccaacaggt
      781 ggcgtggttg ttggggctgt ccgcaatagc tgcgcttatc gcgcagttcg tggtactccg
      841 tccacagcgc ctgactccag tggctctgct cctgagtgcc ggggtgttga tgagtagtgg
      901 tctggctatc atgctcactg aacagctatg gttgttttac ctgggctgtg cagtgctgtc
      961 atttggggcc gctctggcaa cacccgctta tcaactttta ctgaatgata agctggccga
     1021 cggcgcaggc gcgggctggc ttgcggccag tcacacactt ggctatgggc tgtgcgcatt
     1081 gttggtgcca ctggcgtcga aaacaggtgt ctcaatcgcg ctgattgtga cggcattatt
     1141 tgccgctgta ttatttacca tagtgtctgc atgtatttgg cattaccgta caatcaagta
     1201 aggctatgat gttgtactca cagccggcat aacgtcctgt gcaatttcgc tgtcgaaacc
     1261 gcctgtgctg accgcccagt gcactcacct gaagcacatc gcagcatgcc accaggatgc
     1321 cagatcgtct tctgaaatgg gtggatacat tggcaacgtt aaccgcaaca atttttcaga
     1381 gagaattttg gggggtgagg gccagtgatg taaaaaatac gccaagaggt taattcattg
     1441 tttatactga tatgtcaggt aatgcgtttg cagtcattac agtcgtcatg gagactgtaa
     1501 tgactgcaaa cgcataccat ggagcgaaat tctatcctaa gcgtcacatt agttgataaa
     1561 cctgtaacgt taatgaattc tcatgcgaaa agtatggtta tgataaaaaa gcctaaagct
     1621 gtcatgatga gggagccaat gacgtgaaca agtacagacg tcaaagccca tatataattt
     1681 cccgcctgga gtaatgcgaa tacttcaaca gaaaatgtag agaaagtact cagacctcca
     1741 caaagtcccg ttgttatcat taatttccaa aaaggatcaa ggtgtggttg tcgtaaaaag
     1801 tacgccaacg cagtaccaat aattaacccg gcaaggaggt tcaccaccag tgtacctgga
     1861 ggtagatttg gaaaaagact gttaaagcgt gtggacagta accagcgcag agtacatcca
     1921 acagaaccgc cgataatgac ggcaaaaagt gatttaatca tgtacccctc ataaaataat
     1981 tttttatcgt aacaatatc
//
LOCUS       DBANUU010000329         1986 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000329 DBANUU010000000
VERSION     DBANUU010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.335"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..242)
                     /locus_tag="ACIE68_004908"
     CDS             complement(<1..242)
                     /locus_tag="ACIE68_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFX5911053.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACIE68_004909"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACIE68_004909"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFX5911054.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT          543 a          499 c          405 g          539 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtcacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccgcc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 taaacttcga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaggatt tcgacgccat ttcaatcggg ttcaacggcg gtccacctgc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gtagcgcggt ataacgcgtt ggtggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggtg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaagcctaa aaccttatta ttttcactga cagcagatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagggagat acgctttttg ataggaatca atcaattaca
     1981 tgaata
//
LOCUS       DBANUU010000330         1985 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000330 DBANUU010000000
VERSION     DBANUU010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.336"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>1985
                     /locus_tag="ACIE68_004910"
     CDS             <1..>1985
                     /locus_tag="ACIE68_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFX5911055.1"
                     /translation="KAPFDIRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLW
                     LARGGVAAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEV
                     LPPEPPAYRVLTGVVDGFGRTLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRA
                     EVFRKQRATSLSSPAGPRSASSSLVFPDTLPAGTGYGTDNGIRLEAVWLTHDPAYPDE
                     QPTAPLARYTYTAGGELRAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESCYRYDD
                     TGRVTEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSIT
                     RSEYDEAGRLKAQTDAAGRRTEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTY
                     PDGLRSSREYDEKGRLTAETSRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYG
                     QLLAFTDCSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQKDAQGREIRYE
                     YSAAGDLTATISPDGKRSTIEYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQ
                     STFRYDPVDRLTEQRGFDGRTQRYHYDLTGKLTQSEDEGLITLWHYDASDRITHRTVN
                     GDPAEQWQYDEHGWLTTLSHTCEGHRVSVHYGYDDKGR"
BASE COUNT          412 a          546 c          716 g          311 t
ORIGIN      
        1 aaagcgccct tcgatatccg cttacagata cgcgatgaag gcctgatact caacgacagc
       61 ggcggcagga gcattcactt tgagccgctg tttcccggcg agataagcta cagccgcagc
      121 gagtcactct ggctggcgcg gggcggagta gcggcgcagc acagttcgca gccgctaagc
      181 gcgctctggc aggtgctgcc ggaagatgtt cgcctgagtc cgcatgtgta cctggcgaca
      241 aacagccttc aggggccgtg gtggatactc agctggccgg agcgggtgcc gggggcggac
      301 gaggtgctgc cgccggagcc gcccgcatac cgggtgctga cgggcgtggt ggacggcttc
      361 gggcggacgc tggcctttca ccgggcggcg gagggtgatg tggcgggcgc ggtgacgggg
      421 gtgacggacg gcgcgggccg ccgttttcac ctggtgctga ccacacaggc acagcgggcg
      481 gaagtattcc gtaaacagcg cgccacgtct ttatcttccc ctgccggtcc ccgttctgct
      541 tcctcttctc tggttttccc cgacacgctg cccgccggta caggatacgg cacggataac
      601 ggtatccggc tggaggcggt atggctgaca cacgacccgg catacccgga tgaacagccg
      661 actgcgccgc tggcgcgcta cacgtacacg gccggtggtg aactgcgggc ggtgtatgac
      721 cgcagcggaa cgcaggtgcg cgggtttact tatgatgcgg agcacgccgg gcggatggtg
      781 gcgcaccatt atgcggggag gccggagagc tgctaccggt atgatgatac cggtcgggtg
      841 acggagcagg tcaacccgga ggggctggac taccgctttg agtacggaga gagccgggta
      901 atcatcacgg acagcctgaa ccggcgggag gtgctgtaca cggaaggcga gggtggcctg
      961 aagcgggtgg tgaagaagga acatgcggac gggagcatta cccgcagcga gtatgatgag
     1021 gcggggaggc tgaaggcaca gacggatgcg gcggggcggc ggacggagta cagcctgcat
     1081 atggcgtcgg gtgcggtgac ggcggtgacg gggccggacg gcaggacggt acggtatggc
     1141 tataacagcc agcggcaggt gacgtcggtg acgtacccgg acgggcttcg cagcagccgg
     1201 gagtatgatg agaagggaag gctgacggcg gagacctcgc gcagcggtga gacaacgcgg
     1261 tacagctatg atgacccggc gagtgagctg ccgacaggga tacaggacgc gacgggcagt
     1321 acaaaacaga tggcatggag ccgttacggt caactgctgg ccttcacgga ctgctcgggg
     1381 tacacgacgc ggtatgagta cgaccggtac ggtcaacaaa tcgccgttca ccgggaggaa
     1441 ggcatcagca cttacagcag ttataacccg cgtgggcaac tggtcagtca gaaggatgcg
     1501 caggggcgtg aaatccgtta tgagtacagc gccgcaggcg acctgacagc aacgatatca
     1561 ccggacggta aacgcagcac catcgaatat gataaacgcg ggcgtccggt gagtgtgacg
     1621 gaaggcggtc ttacgcgcag catggggtat gacgctgccg ggcgcatcac cgtgctgacc
     1681 aacgagaacg gcagccagtc cacgttccgg tatgacccgg tggacaggct gactgaacag
     1741 cgcggttttg acggccggac gcaacgttac cactatgacc tgaccggaaa actcacgcag
     1801 agtgaagacg aggggcttat caccctctgg cactacgatg cgtcggaccg catcacgcac
     1861 cggacggtga acggcgaccc ggcagagcag tggcagtatg atgagcacgg gtggttaacc
     1921 accctcagcc atacctgtga aggccaccgc gtgtcggtcc actacggcta tgacgataaa
     1981 ggccg
//
LOCUS       DBANUU010000331         1932 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000331 DBANUU010000000
VERSION     DBANUU010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.337"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            1..1932
                     /locus_tag="ACIE68_004911"
                     /pseudo
     CDS             1..1932
                     /locus_tag="ACIE68_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491406.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family protein"
BASE COUNT          681 a          374 c          360 g          517 t
ORIGIN      
        1 ttaccctcaa ggattaaaag acagagaaat tatatcaact tttcacgcag aaaaagttat
       61 taatgatctg ttaaaaaacg attataaaat ttccctttct ccagatacaa cttatcgaga
      121 gttgaataaa gcagcacagc gtagcattac agcgccagac aggataggag aaggaaaatc
      181 atgggtttat caaagagata caatgattga aagaggtgat aacagcggtg ttcatcagta
      241 tggtcctgct gaacatttca cccacattat atctgacaaa ccgtccccaa aagatgagta
      301 tattgcatat gctattaaca ttcctgacta tgagctggca gccgatgtat ataatattaa
      361 cgtgacgtca ccttccgaac agcaagaaac atttaaaata ctaatcaatc cagaacatct
      421 acggcaaaca cttgagcgta aatctcttac tgctgttcag aaatcacaat gtgaaatcat
      481 aacccccaaa aaacctggcg aagcgattct tcatgctttt aatgccacct accagcaaat
      541 cagggaaaat atgtctgagt ttgcacgttg ccattatggg tatatacaaa tccctccagt
      601 gacaactttc cgcgccgacg gaccagaaac tcccgaagaa gaaaagggtt actggtttca
      661 tgcttatcaa cccgaagatc tttgtaccat ccataatcca atgggagatt tgcaggattt
      721 tattgcattg gttaaagatg ctaaaaaatt tggtatcgat atcattcctg attatacctt
      781 taactttatg ggaattgggg gtagtggtaa aaatgacctg gattatccct ctgctgatat
      841 acgagcgaag atcagtaaag atatagaagg tggtatccct ggctattggc aaggtcaggt
      901 tttgattcca ttcattaaag atccagtaac aaaagaacgt aaacaaatcc atccagaaga
      961 tatacatctc actgcaaaag acttcgaagc aagtaaagat aacatctcta aggatgaatg
     1021 ggaaaacctc catgcattaa aagaaaagcg tttaaatgga atgcctaaaa caacacccaa
     1081 aagtgaccag gttattatgt tgcaaaatca atacgttcgt gaaatgcgaa aatatggcgt
     1141 acgaggttta cgttatgatg cggcaaaaca ctcaaaacat gaacaaatag aaagatcaat
     1201 aaccccaccg cttaaaaatt ataatgagcg gttacacaat actaacttat ttaacccaaa
     1261 atatcataaa aaagccgtta tgaattacat ggaatatctg gtaacttgtc agttggatga
     1321 acaacaaatg tcatcactgc tttatgaaag agatgattta agcgccattg atttttcatt
     1381 gctcatgaaa acgataaaag ccttttcatt tggtggagat cttcaaaccc ttgcatcaaa
     1441 accgggttcc acaatctcaa gtatcccatc agaaagacgg atattgatta acattaacca
     1501 cgattttcct aacaatggta atcttttcaa tgactttcta tttaaccatc aacaagatga
     1561 acaattagca atggcatata tagccgctct gccgttcagc aggcctttag tttactggga
     1621 tggccaagta ttaaaatcaa cgactgaaat taaaaattat gatgggtcca cgcgtgtcgg
     1681 cggtgaggcg tagcttaata aaggttgctc tacctatcag cagctctaca atgaattcca
     1741 cgcattatat atagataaag caggaatatg gagcgcattt gagggtgtat ctgcaactaa
     1801 aaacgttctg gcctttagtc gtggggattc tgtgaacatt aatcactctc ctcatgatgg
     1861 actagttata ataaataaag gaaacgaaga agttgaaggt acctggccta acaaattgca
     1921 acctggaata ta
//
LOCUS       DBANUU010000332         1931 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000332 DBANUU010000000
VERSION     DBANUU010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.338"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(15..248)
                     /locus_tag="ACIE68_004912"
     CDS             complement(15..248)
                     /locus_tag="ACIE68_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFX5911056.1"
                     /translation="MSDCHPVLLPEGPFSREQAVAVTTAYRNVLIEDDQGTHFRLVIR
                     NAEGQLRWRCWNFEPDAGKQLNSYLASEGILRQ"
     gene            complement(254..931)
                     /locus_tag="ACIE68_004913"
     CDS             complement(254..931)
                     /locus_tag="ACIE68_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911057.1"
                     /translation="MNTRALFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEIGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTTTINKDSVLQ
                     AARQAYARKYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGADGNATGA
                     SVEFIGK"
     gene            complement(1079..1759)
                     /locus_tag="ACIE68_004914"
     CDS             complement(1079..1759)
                     /locus_tag="ACIE68_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFX5911058.1"
                     /translation="MSDNRSRHDRLAVRLSLIISRLMAGESLSLKTLSDEFGVTERTL
                     QRDFHQRLVHLDLEYRNGRYSLRRQSSPGAIPEMLSFIQNTGIARILPLRNGRLITCL
                     TDNQEPSPCLIWLPAPDITATFPECFSQLILAIRQCIHISLMTERWYPSLEPCRLIYY
                     SGSWYLIALQKGKLQVFPLADIKSVSLTSERFERRGHIHSLVAEERFISALPHFSFIH
                     KLINTFNL"
BASE COUNT          490 a          448 c          488 g          505 t
ORIGIN      
        1 tgaaatgaag acgtttattg cctgagaatt ccctcactgg cgagatacga atttagctgt
       61 tttccggcat caggttcaaa attccagcac cgccagcgta gctgcccttc ggcattgcgg
      121 ataaccagcc ggaaatgcgt tccctggtca tcttcaataa gcacattgcg gtaagctgtt
      181 gtgacggcaa ccgcctgttc gcgtgagaaa gggccttcgg gcaataaaac agggtggcag
      241 tccgacatac cgactatttt ccgataaatt caacgctggc gccggtggca ttaccgtcag
      301 caccaaccgt gacggtcact ttgtcgtacg cacggtcttc aggattgaat acttcaaact
      361 caaccttatt gccttttact tcgtaagagc caaaatcagc ggtttctgtt gtttcctcgt
      421 cgtatttccg ggcgtatgcc tgtcgggcag cctgcaggac ggaatcttta ttaatagtgg
      481 tcgtctgttt cggggaaaaa ccggcaagtg atgtgacaaa tttcggacag accttcagca
      541 tatcaccgcg ggtgaattcc attcgtacac caaatgcctg taaaacggac acatcacgct
      601 ctgctatgga gtgaacggct tttttgacca tttccggcgc ctgtccgatt tccacctcaa
      661 cagccctgaa tcccgtctgc tgattacaca tttgtgatgt ggggatcagg ttaccgttaa
      721 tactgatacg gctgcccagg agcgtctgta caccattcgg gcatccctgc atcaggccgg
      781 taataccaat cgtgccattt ttcagcatta ccagcgcctg accatcttcc gtcatggtct
      841 gacaccagcc gtttttgaca gcccagttgc cggcggaggc ggagaatgat gccacagtga
      901 acagcagggg aaaaagagct ctggtattca tgactgacct ctgaaatgaa cgtacggtgt
      961 tattgataat gatggaaggt atgcagtgac tgttcaggga atgacgtagc cgtatattta
     1021 gttttctgtt caacatattg tctccatacc tgtcgggacg gctttggcag gccggcgatc
     1081 acaggttaaa ggtgttgata agtttatgga tgaaagagaa atgtggcagg gcggagataa
     1141 aacgctcttc agcgaccaga ctgtggatgt ggcctctccg ttcaaaccgt tctgatgtca
     1201 ggctgactga tttgatatct gccagaggaa agacctgcag ttttcccttc tgtaacgcga
     1261 tcagatacca gctaccgctg taataaatga gccggcaggg ctccagtgac ggataccatc
     1321 gctcagtcat cagagagatg tggatacact gtcttattgc caggatgagt tgcgagaaac
     1381 actcggggaa cgttgcagtg atatccggcg ccggtagcca gataaggcag ggagagggct
     1441 cctggttgtc ggtaagacag gttatcagtc gtccgttccg gagcggaagt atccgtgcga
     1501 tcccggtatt ctgtataaaa gaaagcattt cagggatcgc acccgggctg ctctgtcgtc
     1561 tgaggctgta cctgccattt ctgtactcta aatccaggtg aaccagacgc tgatgaaaat
     1621 cgcgctgtaa agtacgttct gtaacgccaa attcatctga cagtgttttt agtgacagag
     1681 attctccggc catcagtcgg ctgataatga gtgataagcg aaccgccagg cgatcatgcc
     1741 gggaacgatt atcagacata gatgtgcact ccgtaagaaa acatctttta tttcatgatg
     1801 ttattatgca tcatgatgtt gaaaggatgt gtcgcatccg gataatcagg aataaaatgc
     1861 agcagaaaaa atattctttt aattatctga tttttaagca tgatttcata aaaagaagaa
     1921 tgctcgttaa t
//
LOCUS       DBANUU010000333         1915 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000333 DBANUU010000000
VERSION     DBANUU010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.339"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..530
                     /locus_tag="ACIE68_004915"
     CDS             <1..530
                     /locus_tag="ACIE68_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFX5911059.1"
                     /translation="PEIILLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHR
                     GNVVDRVIEGAYEVVGVFDRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVT
                     TADILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLN
                     RALWVMAETMLESLR"
     gene            612..1091
                     /locus_tag="ACIE68_004916"
     CDS             612..1091
                     /locus_tag="ACIE68_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFX5911060.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFSAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            1107..1583
                     /locus_tag="ACIE68_004917"
     CDS             1107..1583
                     /locus_tag="ACIE68_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFX5911061.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            1646..1867
                     /locus_tag="ACIE68_004918"
     CDS             1646..1867
                     /locus_tag="ACIE68_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFX5911062.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
BASE COUNT          438 a          528 c          516 g          433 t
ORIGIN      
        1 tccctgaaat tattctgctc aactctcatg acggtacctc cagctaccag atgctgccgg
       61 ggtacttcag gttcgtctgc cagaacggct gcgtctgtgg tcagtctctg ggggaagtac
      121 gtgttccgca ccggggaaat gtggtggaca gagtcattga aggggcttac gaggtggtgg
      181 gcgtgtttga ccggatagag gagaagcgtg atgccatgca gtcgctggtc ctgccgccac
      241 cggcacgcca ggcgctggca caggcggcac tgacttaccg ttatggtgac gaacatcagc
      301 ccgtcaccac cgccgacatt ctgacgccgc gacgccggga ggattacggt aaggacctgt
      361 ggagcgcata tcagaccatc caggagaata tgctgaaagg cgggatttcc ggtcgcagtg
      421 caaaaggaaa acgtatccat acccgggcca ttcacagcat tgataccgac attaagctca
      481 atcgcgcatt gtgggtgatg gcagaaacga tgctggagag cctgcgctga tgccgtttcc
      541 ctgagtgagg gaatcattca gctttcgttc ctgaaaaaca cacatttatt tgttatttaa
      601 ggagttatct catgaaaacc gtttctcaga atacccccac aatttattca gctacaacac
      661 cagagaataa tccgcctcag ttggttgcca gcctcgtccc tgatgaacag cgcatcagct
      721 tctggccgca gcattttggc ctcattccac agtgggtgac cctggagccc cgtgtcttcg
      781 gctggatgga ccgtctgtgc gaagactact gcggtggtat ctggaatctg tacaccctga
      841 acaacggcgg ggcatttatg gcacccgaac cggatgacga tgatgacgaa acatgggtac
      901 tgttcagtgc catgaacggt aaccgcgcag aaatgagccc ggaagccgcc ggtattgccg
      961 cctgtctgat gacgtacagt catcatgcct gtcgtacgga gaattatgcc atgacggtcc
     1021 attattaccg gttgcgggat tacgccctgc agcatccgga atgcagcgcc attatgcgca
     1081 tcatcgactg aatggagaga agcacaatgc aacaactttc ctttctgccc ggagagatga
     1141 cgcccggcga gcgcagcctc attcagcggg ccctgaaaac cctggaccgc catcttcatg
     1201 aacccggtgt ggccttcacc tccacgcatg cggcacggga atggctgata ctgaacatgg
     1261 cgggactgga gcgggaagag ttccgggtgc tgtatctgaa caaccagaat cagctgattg
     1321 ccggtgaaac cctcttcacc ggtaccatca accgcacgga agtccatccc cgggaagtga
     1381 ttaaacgcgc cctgtaccac aatgccgcag ccgtggtgct ggcacacaat cacccgtccg
     1441 gtgaagtcac acccagtaag gcagaccggc ttatcaccga acgtctggta caggcactgg
     1501 gcctggtgga tatccgggtg ccggaccatc tgatagtcgg tggcaaccag gttttctcct
     1561 ttgccgaaca tggtctgctt taacccttca caaccacatc acacctgttt tcacttttat
     1621 cttctgtctt cagaggtatc ccattatgaa aattatcacc cgtggtgaag ccatgcgtat
     1681 tcaccaacaa catccgacat cccgtctttt tccgttctgt accggtaaat accgctggca
     1741 cggcagtgct gaagcgtata ccggtcgtga agtgcaggat attcccggtg tgctggccgt
     1801 gtttgctgaa cgccgtaagg acagttttgg tccgtatgtc cggctgatga gcgtcaccct
     1861 gaactgagtg ggaattctga tgagcagaat tatcgccact accggaccat tctta
//
LOCUS       DBANUU010000334         1907 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000334 DBANUU010000000
VERSION     DBANUU010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.340"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..1082
                     /locus_tag="ACIE68_004919"
     CDS             <1..1082
                     /locus_tag="ACIE68_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911063.1"
                     /translation="GPMQLEVPRDQYAGAVETMKNKIREGKVPGVTDPAEASRLIRRG
                     HLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTASVFWLSTGDRDAALQT
                     AAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPTVRQALQKGTGAGNIS
                     ALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLAVASSGVAGGAVGSVA
                     GGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEEDRVKILAMIQEQVTWL
                     AGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNMLIKPLVVSIIRQRPV
                     MEYDASHLGKMVNRLEEALPPELPA"
     gene            1158..1613
                     /locus_tag="ACIE68_004920"
     CDS             1158..1613
                     /locus_tag="ACIE68_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFX5911064.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPLDYHPLLITVSGKNVN"
     gene            1648..1776
                     /locus_tag="ACIE68_004921"
                     /pseudo
     CDS             1648..1776
                     /locus_tag="ACIE68_004921"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1732..>1907)
                     /locus_tag="ACIE68_004922"
     CDS             complement(1732..>1907)
                     /locus_tag="ACIE68_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911065.1"
                     /translation="STGEPRIKLFSGISFRVPAASASLTFRVADNPTEMHSRVIQDKH
                     IAVSGYDSNRLPV"
BASE COUNT          456 a          488 c          522 g          441 t
ORIGIN      
        1 atggtcccat gcaactggaa gtcccccgtg atcagtatgc cggcgctgtg gaaaccatga
       61 agaataagat ccgcgaaggt aaagtacccg gtgtaaccga tcccgcagaa gcgtcccggc
      121 tgattcgtcg gggacatctg acttataccc aggcccgtaa tatcacccgg ttcgggacca
      181 tcgaatcggt cacttatgat attgccgagg ggtcggttgt cagtctggcg gccggaggga
      241 tcagttttgc cctgacggca tcggtcttct ggctcagcac cggcgatcgc gatgctgccc
      301 tgcagacagc tgctgtccag gcaggaaaaa ccttcacccg cacactggct gtctacgtca
      361 caacccagca acttcaccgg ctcactgttg ttcagggtat gctgaagcat attgattttt
      421 cgacggccag cccgactgtc cggcaggcgc ttcagaaggg gaccggtgca ggaaatatca
      481 gtgccctgaa caaagtgatg aagggtacgc tggtgacatc tctggcactg gtcgctgtca
      541 caaccggccc tgacatgatc aaaatgttgc ggggacggat ctccggtgcg cagttcatca
      601 ggaatcttgc cgtggcatct tccggtgtgg caggtggtgc tgtcgggtca gtggcgggcg
      661 ggatattgtt cagtccactg ggaccatttg gtgcactgac agggcgtgtg gttggcggtg
      721 ttctgggggg aatgattgcc tccgctgtat caggaaaaat tgccggagcg ctggttgaag
      781 aagatcgcgt caaaattctg gcaatgattc aggagcaggt gacatggctt gccggcagtt
      841 tcctgctgac cggacatgag attgaaaatc tgaacgcaaa tctggcccgt gttatcgatc
      901 agaatgctct ggagatcatt ttcgccgccg gtatacaaca acgggccgcg accaatatgt
      961 taatcaaacc actggtggtc agtatcatca ggcaacgccc cgtcatggaa tatgatgcat
     1021 cccatctcgg caagatggtt aaccgactgg aagaagcatt acccccggag ttaccggcat
     1081 aacggctatt ctgcgccttt tacgttttat cacctgattt gtcagtgtca tcgtcatgac
     1141 tcagagaggt aaataccatg attcacctgt tcaaaacctg catgattacc gccttcattc
     1201 tggggttaac gtggtctgcc ccactccggg cacaggatca acgttacatc agtatacgca
     1261 atacagatac gatatggctc ccgggaaata tttgtgccta ccagttccgg ctggataatg
     1321 gcggaaacga tgaaggattt ggccccctca ccatcactct gcaactcaaa gacaaatatg
     1381 gtcagacgct ggtgaccaga aaaatggaaa cggaagcctt tggtgacagt aatgccacgc
     1441 gaaccacaga cgcatttctg gaaacggagt gcgtggaaaa tgtcgccaca accgaaatca
     1501 ttaaagcaac tgaagaaagt aacggccatc gtgtcagtct gccgttatcg gttttcgatc
     1561 ccctggacta ccatccactg ctgattaccg tttccggaaa aaatgttaac tgatccctga
     1621 agtcagtttc atgcttcagc ctctgccata cgttgtggca gaggctattt atcaggagaa
     1681 accagatatg ccgggttaca cacagagtat gtcctggctg aaggatcttt ctcatacggg
     1741 caggcggttg ctgtcataac cgcttaccgc aatgtgttta tcctggatga cccgggaatg
     1801 catttccgtc gggttatccg caacgcggaa ggtcagcgac gctgacgcgg ccggaactct
     1861 gaagctgatg ccggaaaaca gcttaatgcg tggctcgcca gtggagg
//
LOCUS       DBANUU010000335         1828 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000335 DBANUU010000000
VERSION     DBANUU010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.341"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>1828
                     /locus_tag="ACIE68_004923"
     CDS             <1..>1828
                     /locus_tag="ACIE68_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFX5911066.1"
                     /translation="DAKKTAAVVEQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDV
                     ATQLAGGQSPWLILLQQGGQVKDSFGGMIPMFRGLAGALTLPMVGATSLAVATGALAY
                     AWYQGNSTLSDFNKTLVLSGNQSGLTADRMLVLSRAGQAAGLTFNQTSESLTALVNAG
                     VRGGEQFEAISQSVARFSSASGVEVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQI
                     AYVAQLQRSGDEAGALQAANEAATKGFDDQTRRLKENMGTLETWADRTARAFKSMWDS
                     VLDIGRPDTAREMLEKAEKAFDEADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANA
                     QLGLSAATLQADLEKAREMAAKDWAESEASRLKYTEEAQKAYERLQTPLEKYTARQEE
                     LNKALKDGKILQADYNTLMAAAKKDYEETLKKPKQSGVKVSAGDRQEDSAHAALLTLQ
                     AELRTLERHAGANEKISQQRRDLWKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLE
                     YKRQLAALGDKVTYQERLNALAQQADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQ
                     RLKEQYGDNPLALNNVMSEQKKTWAAEDQLRGSWMAGLKSGWS"
BASE COUNT          441 a          464 c          623 g          300 t
ORIGIN      
        1 tgatgcgaaa aaaacagcgg cagtcgttga acagtcgctg agccgacagg cactggctgc
       61 acagaaagcg gggatttccg tcgggcagta taaagctgcc atgcgtatgc tgcctgcgca
      121 gttcactgac gtggccacgc agcttgcagg cgggcaaagt ccgtggctga tcctgctgca
      181 acagggtggt caggtgaagg actccttcgg cgggatgatc cccatgttca gggggcttgc
      241 cggtgcgctc accctgccga tggtcggggc cacctcgctg gcggtggcga ccggtgcgct
      301 ggcgtatgcc tggtatcagg gcaactcaac cctgtccgat ttcaacaaaa cgctggtcct
      361 ttccggcaat cagtcgggtc tgacggcaga tcgcatgctg gtcctgtcca gagccgggca
      421 ggcggcaggg ctgacgttta accagaccag cgagtcactg acggcgctgg tgaatgccgg
      481 tgtgcgtggt ggtgagcagt ttgaggcgat cagccagagt gtggcgcgtt tctcctctgc
      541 atccggcgtg gaggtggaca aggtcgctga agccttcggg aagctgacca cagacccgac
      601 gtcgggactg acagcgatgg cacgtcagtt ccataacgtg acggcggagc agattgcgta
      661 tgttgctcag ttgcagcgtt ccggagatga agccggggca ttgcaggcgg cgaacgaggc
      721 cgcaacgaaa gggtttgatg accagacccg ccgcctgaaa gagaacatgg gcacgctgga
      781 gacctgggca gacaggacag cacgggcatt caaatccatg tgggattcgg tgctggatat
      841 tggtcgcccg gacactgccc gggaaatgct ggagaaagca gaaaaggctt ttgatgaggc
      901 ggacaaaaaa tggcagtggt atcagagccg gagccaccgg cgcggtaaaa catccgcatt
      961 tcttgccaat ctccggggag catgggagga cagagcgaat gcgcaacttg ggctttcagc
     1021 cgccacgttg caggccgatc ttgaaaaggc cagagagatg gcagcaaagg actgggccga
     1081 gtctgaggca tcacggctga aatataccga agaggcgcag aaggcttatg aacgcctgca
     1141 gacgccgctg gagaaatata ccgcccgtca ggaagaactg aacaaggcac tgaaggacgg
     1201 gaaaattctg caggcagatt acaacacgct gatggcggcg gcgaaaaagg actatgaaga
     1261 gacgctgaaa aagccgaaac agtccggcgt gaaggtgtct gcgggcgatc gtcaggaaga
     1321 cagtgctcat gctgccctgc tgacgcttca ggctgaactc cggacgctgg agaggcatgc
     1381 cggagcaaat gagaaaatca gccagcagcg ccgggatttg tggaaggcgg agagtcagtt
     1441 cgcggtactg gaggaggcgg cgcaacgtcg ccagctgtct gcacaggaga aatccctgct
     1501 ggcgcataaa gatgagacgc tggagtacaa acgccagctg gctgcacttg gcgacaaggt
     1561 tacgtatcag gagcgcctga acgcgctggc gcagcaggcg gataaattcg cacagcagca
     1621 acgggcaaaa cgggccgcca ttgatgcgaa aagccggggg ctgactgacc ggcaggcaga
     1681 acgggaagcc acggaacagc gcctgaagga acagtatggc gataatccgc tggcgctgaa
     1741 taacgtcatg tcagagcaga aaaagacctg ggcggctgaa gaccagcttc gcgggagctg
     1801 gatggcaggc ctgaagtccg gctggagt
//
LOCUS       DBANUU010000336         1794 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000336 DBANUU010000000
VERSION     DBANUU010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.342"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(64..723)
                     /locus_tag="ACIE68_004924"
     CDS             complement(64..723)
                     /locus_tag="ACIE68_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFX5911067.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(774..1472)
                     /locus_tag="ACIE68_004925"
     CDS             complement(774..1472)
                     /locus_tag="ACIE68_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFX5911068.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(1491..>1794)
                     /locus_tag="ACIE68_004926"
     CDS             complement(1491..>1794)
                     /locus_tag="ACIE68_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFX5911069.1"
                     /translation="NDYGDLQINILFTSRQMIIETFICPVSSISNPDEFNTFLLRNQK
                     MMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILLEITSLVDNALDLAEITEEYSH"
BASE COUNT          464 a          448 c          378 g          504 t
ORIGIN      
        1 taaaaaaata ggcgctaaag gcaagaagtg aatccagtat atgcattgta aacgtccttg
       61 taattaacca ataatatgaa gtgatgtcag cgggatatcg acacccaacg cgcgcgaaaa
      121 taaccactct ggctcgccga gatcgttaaa agattcctca ccatttaaca gcaaatattc
      181 gtaagtatct tcggttactt ggcgttggta gcccatggta aaattatgga cctcccattt
      241 ggcatggttt tgattttcta ctttttccag catgtagacg ggttcgatat ttcccggttc
      301 ttcgctgtta aaaaatcgct gccagcgttt ttcctgccag tttaagatca ttgcccccat
      361 cactttggcg ttgatcgcct cgcgccagtg actctcttta ctgataccgt agctctcttc
      421 atagacaaag agcttgatat catcaatgtc atcaatatct tcgccgccgg tggtattgat
      481 ttgcagaaat tcatcgcctg aagtgtagta gcggaaaatc tgactaccgc cgcccagatc
      541 gatgtggctg acagcggcta ccgtaaattc ctcacccggc agcgcaatca gcaattcatc
      601 ttccagcaaa cgaaacgcta acgtgtcgag cgtaaagcca ctattgagat gaagtcccag
      661 cggaccgcga gcgatagcag gcttattatc cttgccaaac agacgctgga aaaaaccaga
      721 catattttat ccttaaaata gttgccagcc ttttagcgag gctggcaaaa aaattactcg
      781 ccctgttggc gttgcagtct tgctaataca tcctgggcgc tacttttatt gctaccgcca
      841 attccggctt ccgccagctt ttcatcaaga tcgcgaccgt ctgcgacttt ttccaactgt
      901 gcagcagcat ccaggcgagc ctgacgttcg gcctgacgcg tttgcaggcg ttttaaggat
      961 tctgccgctg tcgaaacgct ggaagatgcg ccaacggtag aggttgttac agcctgttgt
     1021 gcacgctgca tggcttcagt ggctttaacg acttccattt gctgctcaaa ctgagcgata
     1081 cgctgcgctg tcgctgtaac tgctttttcc acgccatcac gggagacttc cagattcgac
     1141 aacacttgct cttcagcggt aatgagattc tcaaggcgcg cgatttcttc agcaacttcg
     1201 ttaatcaacg acggattaac gttcttgctt aacgcttcca gcgcacgggc ttccagactg
     1261 gctttgcgct cacgtaaatc tttcagctta tcgtgactta atttcacccg cgccagcaga
     1321 tcaacgcgag attttccggc tttatcaagt tcagctttag cgtcacgaat atgctgttcc
     1381 agcatgcgca cgccctgggt ttcttcaatg gattcttccg cctgggagat aaacgatttc
     1441 cccagcgtaa ataaactttt taaaattccc ataaataccc gtccttgtaa ttagtgtgaa
     1501 tattcttctg taatttcagc cagatccaat gcgttatcta ccagcgaggt tatctcaagc
     1561 aggatatctt caagagaaga tttaagcgat aacgccccga aaacaatgta atactcttcc
     1621 tgttgcacgc tggagattcc taccgatgac aacggcatca tcttctgatt tcttaataag
     1681 aaggtattaa attcatctgg attgctgata ctacttaccg gacaaataaa ggtttcgata
     1741 atcatttggc gggaagtaaa aagaatattg atttgcaaat cgccataatc attc
//
LOCUS       DBANUU010000337         1761 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000337 DBANUU010000000
VERSION     DBANUU010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.344"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            280..1014
                     /locus_tag="ACIE68_004927"
     CDS             280..1014
                     /locus_tag="ACIE68_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HFX5911070.1"
                     /translation="MSDHTIPEYLQPALAQLEKARAAHLENARLMDETVTAIERAEQE
                     KNALAQADGNDADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACDSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSILVQENPLAN
                     TTGHQGYVAPDKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQGAELKARGLLS"
     gene            1011..1511
                     /locus_tag="ACIE68_004928"
     CDS             1011..1511
                     /locus_tag="ACIE68_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFX5911071.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDCKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAERLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            1585..>1761
                     /locus_tag="ACIE68_004929"
     CDS             1585..>1761
                     /locus_tag="ACIE68_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFX5911072.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRK"
BASE COUNT          474 a          444 c          475 g          368 t
ORIGIN      
        1 gagcgggtga acagtggtga acagacggtg aacagtcatt actgcgattg ttcaccattt
       61 aacttactgt attacttatc ttttttatta aggtgaacag aggtgaacag taaaatataa
      121 aaaaaacaaa cagtaagccg gtttttcctg cgaccttttc ctggcttgcc ggtgtgagga
      181 tgagtctcct gtgtcagggc tggcacatct gcaatgcgtc gtgttgttgt ccggtgtacg
      241 tcacaatttt cttaacctga agtgacgagg agccggaaaa tgtctgacca cactatccct
      301 gaatatctgc aacccgcact ggcacaactg gaaaaggcca gagccgccca tcttgagaac
      361 gcccgcctga tggatgagac cgtcacggcc attgaacggg cagagcagga aaaaaatgcg
      421 ctggcgcagg ccgacggaaa cgacgctgac gactggcgca cggcctttcg tgcagccggt
      481 ggtgtcctga gcgacgagct gaaacagcgc cacattgagc gcgtggcacg ccgggagctg
      541 gtacaggaat atgacaatct ggccgtggtg ctgaatttcg aacgtgaacg cctgaaaggg
      601 gcgtgtgaca gcacggccac cgcctaccgg aaggcacatc atcaccttct gagtctgtat
      661 gcagagcatg agctggaaca cgccctgaat gaaacctgtg aggcgcttgt ccgggcaatg
      721 catctgagta tcctggtaca ggaaaatccg ctcgccaaca ccaccggcca tcagggctac
      781 gtcgcacccg ataaagctgt catgcagcag gtgaaatcat cgctggaaca gaaaattaaa
      841 cagatgcaaa tcagcctcac cggcgagccg gttctccggc tgaccggact gtcagcggca
      901 acactcccgc acatggatta tgaggtggca ggcacaccgg cacagcgcaa ggtgtggcag
      961 gacaaaatag accagcaggg tgcagagctt aaggccagag gactgctgtc atgatttact
     1021 gcccgtcgtg tggacatgtt gctcacaccc gccgcgcaca tttcatggac gatggcacca
     1081 agataatgat tgcacagtgc cggaatattt attgctctgc gacatttgaa gcgagtgaaa
     1141 gctttttctc tgactgtaaa gattcaggaa tggaatacat ttcaggcaaa cagagatacc
     1201 gcgattcact gacttctgcc tccggcagta tgaaacgccc gaaaagaatg cttgttaccg
     1261 gatattgttg tcggagatgt aaaggccttg cactgtcaag aacatcgcgg cgtctgtctc
     1321 aggaagtcac cgaacgtttt tatgtgtgca cggatccggg ctgtggtctg gtgtttaaaa
     1381 cgcttcagac catcaaccgc ttcattgtcc gcccggtcac accggacgaa ctggcagaac
     1441 gcctgcatga aaaacaggaa ctgccgccag tacggttaaa aacacaatca tattcgctgc
     1501 gtctggaatg agggctgccg gttaacaccg gccgtcgccg cacaccgtat ttttattctt
     1561 cagcatgatg agaaagagat aacgatggaa agcacagcct tacagcaggc ctttgacacc
     1621 tgtcagaata acaaagcagc atggctgcaa cgcaaaaatg agctggcagc ggccgaacag
     1681 gaatacctgc ggcttctgtc aggagaaggc agaaacgtca gtcgcctgga cgaattacgc
     1741 aatattatcg aagtcagaaa a
//
LOCUS       DBANUU010000338         1747 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000338 DBANUU010000000
VERSION     DBANUU010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.345"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..507
                     /locus_tag="ACIE68_004930"
     CDS             <1..507
                     /locus_tag="ACIE68_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFX5911073.1"
                     /translation="VAALNGVDVRAFTGQAKFCKKAAAGYSNCCKDSGWGQDIGLAKC
                     SSDEKALAKAKSNKLTVSVGEFCSKKVLGVCLEKKRSYCQFDSKLAQIVQQQGRNGQL
                     RISFGSAKHPDCRGITVDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIA
                     DQLKQAGQ"
     gene            534..791
                     /locus_tag="ACIE68_004931"
     CDS             534..791
                     /locus_tag="ACIE68_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFX5911074.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTAGEIL
                     AAAFVIFYSGVLLFILYFCFSALTDLQKPDERKSDERNEDE"
     gene            784..1527
                     /gene="traF"
                     /locus_tag="ACIE68_004932"
     CDS             784..1527
                     /gene="traF"
                     /locus_tag="ACIE68_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFX5911075.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQVQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            1543..>1747
                     /locus_tag="ACIE68_004933"
     CDS             1543..>1747
                     /locus_tag="ACIE68_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFX5911076.1"
                     /translation="MSEDCLKMFTGVVLLIFVIIAGYFFSERNDRKMFLLSSLVFLVI
                     NIACLYVLTASLWFLCGAIMNQGA"
BASE COUNT          453 a          375 c          468 g          451 t
ORIGIN      
        1 gttgcggcgc tgaacggggt ggatgtccgt gctttcaccg gtcaggcgaa attttgtaag
       61 aaggctgcag ccggatacag caactgctgt aaggacagcg gctggggaca ggatatcggg
      121 ctggccaaat gcagcagtga tgaaaaagcc ctggctaaag caaaatcaaa caaactgacc
      181 gtcagtgtcg gagagttctg ttcgaagaaa gtgctgggtg tctgcctgga gaaaaaacgc
      241 agttactgtc agtttgattc gaagctggcg cagatagtcc agcagcaggg gcgcaacgga
      301 cagctgcgta tcagttttgg cagtgcgaag catcctgact gtcgggggat tacggttgat
      361 gagctgcaga aaattcagtt cgacagactg gatttcacta acttctacga agacctgatg
      421 aataaccaga aaatcccgga cagtggtgtt ctgacgcaga aagtgaaaga gcagattgct
      481 gaccagctga aacaggcagg acagtaaggt agggaaaata accggggaaa atgatgaaag
      541 tcatattcac gtcgaaccgt tttattgatt ttcttatccg gcttttgatt acagcgattg
      601 tcatttcccc ggtaattatc tggtcgtggg atacggtaaa agaaaccact gcaggtgaga
      661 tacttgctgc tgctttcgtt attttttact ccggtgtgct cctgtttatt ctgtattttt
      721 gtttttctgc cctgactgac ctgcagaaac ctgatgaaag aaaaagtgat gaaaggaatg
      781 aagatgaata aagcattact gccactgtta ctctgctgct ttatttttcc ggcgtcagga
      841 aaagatgcag gctggcagtg gtataacgag aaaataaatc cgaaggaaaa agaaaataaa
      901 ccagtacctg cagccccccg tcaggagccg gacattatgc agaaactggc cgcactgcag
      961 acggcaacaa agcgggcgct gtacgaagct attctgtatc ccggcgtgga taattttgtg
     1021 aaatatttcc ggctgcagaa ttactggact cagcaggccg ggcttttcac catgagcgcc
     1081 aaaaaagcca tgctggcgca tcccgaactg gactataacc tgcagtacag ccattacaac
     1141 ggcacggtcc ggaaccagct ggcggcagac caggtgcagc agcgacaggc cattgcgaaa
     1201 ctggctgaac actacggcat catgttcttt taccgggggc aggaccccat cgacgggcag
     1261 ctggcgcagg tcattaatgg cttcagggat acctatggtc tgagtgttat tcccgtttcc
     1321 gtggatggcg tgattaatcc gctgttgccg gattcccgga ctgaccaggg gcaggcgcag
     1381 cgcctcggcg tgaaatattt cccggccatg atgctggttg acccgaaaca gggcagtgtt
     1441 cgcccgttat catacggttt tatttcgcag gacgacctgg caaaacaatt cctgaacgtt
     1501 tctgaagatt ttaaacccaa tttttaatcg cggatttgat ttatgagtga agattgtttg
     1561 aaaatgttta caggtgttgt tctgttaata tttgtcatta ttgccggtta tttcttttct
     1621 gagcgtaatg acaggaaaat gtttctcctg agctcactgg ttttccttgt tattaatatc
     1681 gcgtgtttat atgtactgac tgccagtctc tggtttctgt gtggtgcaat tatgaatcag
     1741 ggcgcag
//
LOCUS       DBANUU010000339         1742 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000339 DBANUU010000000
VERSION     DBANUU010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.346"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            38..1018
                     /locus_tag="ACIE68_004934"
     CDS             38..1018
                     /locus_tag="ACIE68_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFX5911077.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            1011..1427
                     /locus_tag="ACIE68_004935"
     CDS             1011..1427
                     /locus_tag="ACIE68_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFX5911078.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(1429..>1742)
                     /locus_tag="ACIE68_004936"
     CDS             complement(1429..>1742)
                     /locus_tag="ACIE68_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /protein_id="HFX5911079.1"
                     /translation="DIIAAACRALNHVWREGYRYMKAGVMLADFTPSGIAQPGLFDEI
                     QPRKNSEKLMKTLDELNQSGKGKVWFAGRGTAPEWQMKREMLSQCYTTKWRDIPLARL
                     G"
BASE COUNT          470 a          356 c          421 g          495 t
ORIGIN      
        1 gttttttcta tttgtttatg agcaacggag actggttatg aaaatcttta ttgatgatgg
       61 ttctaccaac attaaacttg cctggttgga ggatggtgac gtgaaaacgt taatcagccc
      121 taacagcttt aaaccggaat ggtcttttag tttactggat gatgctgctc cggctaatta
      181 cgagatcgac ggcgagaagt tttcatttga tccattaagt gctgatgctg tcgtaacgac
      241 tgaaacacgc tatcagtaca gtgatgtgaa tgttgtggcc attcagcatg cgctacagca
      301 gaccggcctg aaagcgcagc ctgttgatgt catcgtcaca ttgccgatct ctgaatacct
      361 tgacgccaac aatcagaaaa ataagcaaaa cattgaaaga aagaaaaaaa atgtgatgcg
      421 tgaagtacgg gtgcagggga gtgatgcatt tgttattcgc agtgtttccg ttcttcctga
      481 aagtattccc gctggtttca gtgttcttgc ggggcttgag gatgatgaat cccttttgat
      541 cgtagatctc ggtggtacaa ctcttgatgt gtcgcatgtt cgtagcaaaa tgacagggat
      601 tacaaaaacc tggtgtgatc cgaatatcgg cgtgtccctg attacatccg gggttaagga
      661 acagatggct gtacatgcta atacaagagt gagctcattc caggctgaca atatcattgt
      721 gcaccgtaat gaacctgact acctttcccg tcgcatttat aatgctgaac agcgtgagtc
      781 tatcattaat gttatcaatg aacggcagaa gttgcttatt aaacgagtta atgatgtcat
      841 cagccgtttc accgactata cacatgtaat gtgtgttggt ggtggggctg aaattgtggc
      901 cgaggctgtg aagaacctga ctaaggttcc tgatgagcgc ttttatctga gctcatcccc
      961 gcagtttgat ttagtcatgg gaatgataaa aatgaaaggt ggtgtgacta atgagtgatg
     1021 aaaataaatc cagacgctgc tcatttgaac ttttcccgga tgaacggaca ggagataaga
     1081 tagcagatga gctcattgca aatgaaaaac tgaaggagcg gggacgtttc atgcgcgcga
     1141 tgttagttac tggtgcggcg tttgcagcta tagataaacg tcttcctttg ctgatatcgg
     1201 agttactgac agaaaatacg actctggatg atattaataa ggtgatttcc agcgtaatac
     1261 caggtgcttt ttcggttgaa aagaaattgc ttgaattact ggaaaaacaa tctggtctcc
     1321 atacgtctgt ggattgcagt actcctctta cagagcagag tctgtcgaga aatgacggtg
     1381 aagaccagac cagaagaaac gctgaaaata tgttcggaga tgactgaact aacccagcct
     1441 ggccagggga atatctcgcc atttagttgt ataacactga ctcaacattt cccgtttcat
     1501 ttgccattca ggggcggttc ctcgccccgc aaaccacact ttcccttttc ccgactggtt
     1561 cagttcatcg agtgttttca ttaacttttc actgttttta cggggctgga tttcatcaaa
     1621 taatcccggc tgcgctatac ccgatggtgt gaaatcagcc agcatgactc ctgccttcat
     1681 atagcggtac ccttcacgcc agacatggtt taaggctctg catgcggcgg caataatgtc
     1741 cc
//
LOCUS       DBANUU010000340         1741 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000340 DBANUU010000000
VERSION     DBANUU010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.347"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(117..>1741)
                     /locus_tag="ACIE68_004937"
     CDS             complement(117..>1741)
                     /locus_tag="ACIE68_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFX5911080.1"
                     /translation="DYTWNDNGQLVRISGPQECREYRYSGTGRLTGVHTTAANLDIDI
                     PYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAEKTDRIPEGVI
                     RMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGW
                     MSLSRKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKARHRS
                     LAEVLQEDTGVTLPAELAVMLGRLERELRAGAVSAESEAWLAQCGLTVEQMESQMEAE
                     YIPERRLHLYHCDHRGLPQALISPEGETAWRGEYDEWGNQLNEENPPHLYQPYRLPGQ
                     QYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGINLYTYPLAPIRYTDPLGLERVIS
                     VYGPPAPDRAGAETPLVLTDMTGGVTIYYDPETGDSMTFDCSNRIDRRSQRGAGDPYT
                     GEVVGCETNGSGISAAYGTTKIYTTDTRARWLHGGGSSLRDPYAPRQGWKPTMGCTRA
                     QNEDVDELCKKVTSWMYSHPGERIRYERFKTR"
BASE COUNT          336 a          560 c          407 g          438 t
ORIGIN      
        1 gttttgatag tttttctacg ccaccatcag gagcgggtaa tatacatcgt ttaagaactt
       61 tagcatttgc acatccaata accaaaatta gaatgcacac tcccatgtta atcgcattaa
      121 cgtgtcttaa aacgttcata tctaattctc tctccaggat gcgaatacat ccaacttgtc
      181 actttcttac acaactcatc tacatcttca ttctgtgcac gggtacatcc catagttggt
      241 ttccagcctt gtcgaggagc ataaggatcg cgcaacgatg atcccccacc atgtagccat
      301 cttgctctag tatctgtagt atagatcttc gttgtgccat atgctgccga tattcctgat
      361 ccatttgtct cacaaccaac gacttcacct gtatatggat caccagcacc acgttgagaa
      421 cgacgatcaa ttctattgct acaatcaaaa gtcattgaat cacctgtttc aggatcataa
      481 taaatagtca ctcctccagt catatcagta agaacaagag gagtttcagc acctgcccgg
      541 tctggagcgg gaggcccata tacacttata actcgttcta accccagtgg atcagtgtaa
      601 cgtattggtg ccagtggata cgtgtataga tttattcccc ccttcaaccc tatcggatcc
      661 tgggtgatat accgcccctg caacggatcg tagtaccggt gacggttgta atacagtccc
      721 gactcctcat catactgctg ccccggcaaa cggtacggct ggtacaggtg aggcgggttc
      781 tcctcattaa gctggttccc ccactcgtca tactccccgc gccacgccgt ttccccttcc
      841 gggctgataa gcgcctgcgg cagtccccgg tggtcacagt ggtaaagatg cagcctccgc
      901 tccgggatgt attccgcctc catctggctt tccatctgct ccaccgtcag cccgcactgc
      961 gcaagccatg cctcactctc cgcactcacc gcacccgcac gcagttcccg ctccagcctc
     1021 cccagcatca ccgccagctc cgccggaagc gtcacccccg tgtcctcctg caacacctcc
     1081 gccaggctcc ggtgccgcgc cttcgcctgt tcgccattct ctgtttcgat tctcaggagc
     1141 ggcgtgaagc ttcccggctg gtataccgtc tggattcttg tctgttgggt ctgtacagtg
     1201 gtcagcctgt caccgtccca cccgtaccag gtctcctccg gtttacgcga cagcgacatc
     1261 caccccgtca ggtcacgctc ccgccgccat acccgttttc ccgtccggcg gcccagcggg
     1321 tcatagaggt agcggctctc cacctgtggt tcgccgtgct gtatccgcgt gtagaacacc
     1381 agacggtgct ggctgtcgta atggtagtgg tgggtccgct cgtcgtgcat ccggataacc
     1441 ccttccggga tgcggtccgt cttctccgcc agcctgccgt attcatcgta gcgatagaca
     1501 tagtgcgcat cttccgcgat gcggttatcc ggccacgccg tgagggtgct gtccgggtgc
     1561 agttccgggt ccggcagccg gtttcctgcc gggtccgtcg catacgggat atcgatatcc
     1621 aggttcgctg ccgtagtgtg gacgcccgtc agccttcccg tgccgctgta acggtactcc
     1681 cggcactcct gcgggccgct gatgcgcacc agttgtccgt tatcgttcca cgtatagtca
     1741 c
//
LOCUS       DBANUU010000341         1741 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000341 DBANUU010000000
VERSION     DBANUU010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.348"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..602
                     /gene="traX"
                     /locus_tag="ACIE68_004938"
     CDS             <1..602
                     /gene="traX"
                     /locus_tag="ACIE68_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFX5911081.1"
                     /translation="NLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRL
                     WGWGIIAQFAYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPL
                     SGTSYGIAGLLMLAVSHRLYRAEDRAERLALVACLLAVIPVLNLATSDAAAVAGLVMT
                     VLTVGLVSCAGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            657..1217
                     /locus_tag="ACIE68_004939"
     CDS             657..1217
                     /locus_tag="ACIE68_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFX5911082.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEV
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            1336..1548
                     /locus_tag="ACIE68_004940"
     CDS             1336..1548
                     /locus_tag="ACIE68_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911083.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAAHCHRRCRGRV"
BASE COUNT          409 a          411 c          545 g          376 t
ORIGIN      
        1 tcaatctgat attccagctg aagcaggaat ggatgtttct ggccgggcga ggagcctttc
       61 cgctgtttgc cctggtgtgg gggctgaatc tgtcccgtca tgcgcatatc cggcaaccgg
      121 ccattaaccg gctgtgggga tggggaatta ttgcccagtt cgcatattat ctggcaggct
      181 ttccctggta tgaggggaat atcctgtttg cctttgcagt ggcggcacag gtgctgacgt
      241 ggtgtgaaac gcgcagtggg tggcgtacag cagcggccat tcttctgatg gccctgtggg
      301 ggcctttgtc cggcaccagt tacggcattg ccgggctgct gatgctggca gtcagccacc
      361 ggctgtaccg ggcagaagac agagcggaac gtctggcgct ggtcgcctgc ctgctggctg
      421 tcattccggt gcttaacctt gccaccagtg atgcagcggc ggtggccggt cttgtgatga
      481 cggtgctgac cgttggtctg gtgtcgtgtg caggaaaatc attaccccgt ttctggcccg
      541 gggatttttt cccggtattc tacgcctgtc atctggctgt gctgggcgtt ctggcgctgt
      601 gacgggtgtg gtatctttgg ccgcaagagg atgattcgtc agaggcagaa cacagcatga
      661 cagagcagaa gcgaccggta ctgacactga agcggaaaac ggaaggagag acgcctgtcc
      721 gcagccggaa aaccatcatc aatgtcacca cgccaccaaa gtggaaggtg aaaaagcaga
      781 agctggccga aaaggcggcc cgggaagctg agctggcggc aaaaaaagcg caggccagac
      841 aggcactgtc catttatctg aacctgccca cactggatga ggccgtgaac accctgaagc
      901 cctggtggcc gggattgttt gacggtgaca caccccggct tctggcctgc ggtatccggg
      961 acgtgttact ggaagacgtg gcgcagcgga atatcccgct ctcgcataaa aaactgcgca
     1021 gggcgctgaa ggccatcacc cgttcagaaa gctatctgtg tgccatgaaa gccggtgcct
     1081 gccggtatga cacggaaggg tatgtgacgg agcatatttc tcaggaggag gaagtgtatg
     1141 cggcagcgcg tctggataaa atccgccgcc agaacaggat aaaggcagag cttcaggccg
     1201 tgcttgatga gaaataaaaa aaagcctcca tcagagagga ggcagggaaa taaggcttaa
     1261 aaggaaacca aaggaagcca attcagtaga ttatttaaca ccataatact gacgggttaa
     1321 agaggagtga taaagatgaa tggattcaga aacagttcac ggaacggtca ggtctggcgc
     1381 tggcagcgtg ccggaagccg ggccgttatt ctggaggtca gtggacgctg gatggaagcg
     1441 gcagaagcat ggcgacgggc tgccggtgtt gcgccccgga cagactggca gcagtttgcc
     1501 cgaaaaaggg ctgcacactg ccaccggcgc tgccggggaa gggtgtaacg aaaaaagcag
     1561 ccttcattac tccaggagtc agaaggctga ataatgcatg agatttactt ttttaatctc
     1621 cggtcgggca gtatgaccgg acagttctga gtagcacaga gttttttccg tattaagtcg
     1681 cgattttata ttttgtgatt aatttcacaa aataaggttt tattcaatgt gtgctgcgat
     1741 a
//
LOCUS       DBANUU010000342         1711 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000342 DBANUU010000000
VERSION     DBANUU010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.349"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..534
                     /locus_tag="ACIE68_004941"
     CDS             <1..534
                     /locus_tag="ACIE68_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFX5911084.1"
                     /translation="SACVRATAIESTAQRCKKNPIYSPKIVEQSQITAFDKSTNVRRA
                     TAFAISVINDKATIPLLINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEMLQDKN
                     EEVRIEAIIGLSYRKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYK
                     FDDNEIITSAIDKLKRS"
     gene            562..1520
                     /locus_tag="ACIE68_004942"
                     /pseudo
     CDS             562..1520
                     /locus_tag="ACIE68_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1532..>1711
                     /locus_tag="ACIE68_004943"
     CDS             1532..>1711
                     /locus_tag="ACIE68_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFX5911085.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEAS"
BASE COUNT          532 a          321 c          409 g          449 t
ORIGIN      
        1 agcgcttgcg ttcgagctac ggcaatcgag tcaacggctc agcgatgcaa gaaaaaccca
       61 atttattcac ctaaaatagt agaacaatct caaattactg cttttgataa atcgactaat
      121 gtcagacgtg ctacagcatt tgctatttct gttatcaatg ataaagcaac aattccacta
      181 ttgattaatc tgttaaaaga tccaaatgga gatgtcagaa actgggccgc atttgcaata
      241 aatatcaata aatatgataa tagtgatatt agggattgtt ttgtggagat gcttcaggat
      301 aaaaatgagg aagtccgtat tgaagcaata atcggacttt cctacagaaa agataaaagg
      361 gttttatctg ttttatgcga tgagttaaaa aaaaatactg tttatgatga tatcattgaa
      421 gctgcgggtg aattaggtga taaaacgcta cttcctgttt tagatactat gttgtacaag
      481 tttgatgaca atgaaattat aacttccgct attgataagc tgaagcgttc atagcgtgag
      541 ttgcctatgc acagtggggg attcccgccg gcacggtgca gatgctcgac cggctggaaa
      601 gtgaaatcct ggctgaccgg gtgagtgagg aaagccgccg ctggctggca tcgtgcggcc
      661 tgaccgtgga gcagatgaaa aaccagatgg acccggtgta cacgccggcg cgaaaaatcc
      721 acctgtacca ctgcgaccat cgcggcctgc cgctggcgct catcagcacg gaaggggcaa
      781 cagcgtggtg cgcagaatat gatgaatggg gcaacctgct gagtgatgag aacccgcatc
      841 atctgcagca gctcattcgc ctgccgggtc agcagtatga tgaggagtcc ggcctgtatt
      901 acaaccgcca ccgctattat gacccgctgc aggggcggta tatcactcag gatccgattg
      961 ggctgaaggg gggatggaat ttttatcagt atccgttgaa tccggtcata aatgtagatc
     1021 cgcaaggttt ggttgatata aatttatacc ccgaaagtga tcttatccat tctgtagctg
     1081 atgagattaa tatcccaggc gttttcacaa tcggggggca tggtaccccc acatctattg
     1141 aatccgcaac gcgcagtatc atgacagcta aagatctagc atatctaatt aaatttgatg
     1201 ggaattataa agatgggatg acagtttggt tattttcttg taatacaggt aaaggacaaa
     1261 attcatttgc tagccaatta gctaaagagt tacatacaaa tgtaatagga cctgacacgc
     1321 tatggacgtg gtgggggcga ggaactaatg gtaagttaaa aatggataca gtgctaacag
     1381 caccaacgaa ccttaattca aataaggatc taatggctat aacaacaaaa gaccttggta
     1441 attggataac atatgggcca tctgggcacc ccatttctaa tatgcaaggt acgccagaaa
     1501 aacccagtga tataagatag gttgtagatg tatgaaagca tgcttgttac tattttttta
     1561 tttttctctt attggtcaat tgcatggtgc tgatgtgaaa ataaaagaaa acgaaagtgt
     1621 gatgggatct acagcaatga cctatgattt aagtgaagaa aagctgatga aactgaagta
     1681 taaatcacaa catggtgatt cagaggcatc a
//
LOCUS       DBANUU010000343         1703 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000343 DBANUU010000000
VERSION     DBANUU010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.350"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            169..384
                     /gene="essD"
                     /locus_tag="ACIE68_004944"
     CDS             169..384
                     /gene="essD"
                     /locus_tag="ACIE68_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFX5911086.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            389..700
                     /locus_tag="ACIE68_004945"
     CDS             389..700
                     /locus_tag="ACIE68_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFX5911087.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     L"
     gene            697..1230
                     /locus_tag="ACIE68_004946"
     CDS             697..1230
                     /locus_tag="ACIE68_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFX5911088.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            1227..>1703
                     /locus_tag="ACIE68_004947"
     CDS             1227..>1703
                     /locus_tag="ACIE68_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HFX5911089.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLR"
BASE COUNT          477 a          352 c          436 g          438 t
ORIGIN      
        1 ttctcgtatt attattttat atatgacttt ctttcaaaat aattacccac atttttaatg
       61 tgtatgtttt tttagcgccg ttgagaacaa cgtgtgctgt caaaactacc ccgtagactc
      121 cgatcttttc aaacatattg caccatccgt gtacatcggg gtgaggatat gaaatcaatg
      181 gataagttaa caacaggtgt tgcctatggc acatcggcgg gtaatgctgg tttctgggca
      241 ttgcagttac tcgataaagt aactccgtca cagtgggctg caatcggtgt gctgggtagc
      301 ctggtttttg gcctgctgac gtatctgaca aatctttatt tcaagattaa agaagacagg
      361 cgtaaggctg cgagaggaga gtaatccaat gactcaagac tatgaactgg ttgtgaaagg
      421 agtccgtaat tttgagaata aagttacggt aactgtagcc ttacaggaca aagaacgctt
      481 tgacggtgaa atttttgacc tggatgtcgc catggaccgt gttgaaggag ctgcgctgga
      541 gttttatgag gcagcagcca gaaggagcgt ccggcaagtc ttcctggaag tagcagaaaa
      601 attgtcagaa aaagttgagt cttatctgca gcatcagtac tcctttaaga ttgaaaatcc
      661 tgccaataag cacgagcgtc ctcatcataa atatctatga acacaaaaat cagatacggc
      721 ctgtcggctg ccgttctggc gctgattggt gctggcgcat ctgctcctca gatacttgac
      781 cagtttctgg acgaaaaaga aggtaaccac acaatggcat accgcgatgg ttctggcata
      841 tggaccatct gtcggggtgc cacagtggtg gatggaaaaa ccgtttttcc caatatgaaa
      901 ctgtcgaagg aaaaatgcga ccaggtcaac gccattgagc gtgataaggc gctggcatgg
      961 gtggagcgca atattaaagt accactgacc gaaccacaaa aagcgggtat cgcgtcattt
     1021 tgtccctata acattggccc cggtaagtgt ttcccgtcga cgttttataa gcggctgaat
     1081 gctggtgatc gtaaaggtgc atgcgaagcg attcgctggt ggattaagga tggcggacgc
     1141 gattgccgca ttcgttcaaa taactgttac ggtcaggtta ttcgtcgtga ccaggagagc
     1201 gcattaacct gctgggggat agaacagtga atcagatatt catggtgatt tttctcgtgt
     1261 tgtcaggatt tatcgtcgga aatgtctgga gcgaccgagg atggcaaaaa aaatgggcgg
     1321 aacgtgatgc tgccgcatta tcacaagagg taaatgctca atttgctgct cgaataattg
     1381 aacaggggcg aactatagcc cgtgatgagg ctgttaaaga tgcgcaacag aaatctgctg
     1441 aaatttctgc cagggctgct tatctgtctg atagtgttaa ccagttgcgt gccgaagcaa
     1501 aaaaatatgc catacgcctt gacgcagcga agcataccgc agatcttgcc gctgccgtca
     1561 gaggcaaaac aaccaaaacc gccgaaggaa tgctcaccaa catgctcgga gatattgcag
     1621 cagaagctca gctttatgct gaaattgctg acgaacgcta catcgcagga gtgacttgtc
     1681 aacagatcta tgaatcttta aga
//
LOCUS       DBANUU010000344         1686 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000344 DBANUU010000000
VERSION     DBANUU010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.351"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            112..987
                     /locus_tag="ACIE68_004948"
     CDS             112..987
                     /locus_tag="ACIE68_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase
                     CTX-M-1"
                     /protein_id="HFX5911090.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAVAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVDGTMSLAELSAAALQYSDNVAMNKLISHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGDYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTNGL"
     gene            complement(1034..1366)
                     /locus_tag="ACIE68_004949"
     CDS             complement(1034..1366)
                     /locus_tag="ACIE68_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HFX5911091.1"
                     /translation="MRPHRHPHTFELLLPLRGRFVVLNFDDRGTVTHRAILGETCTVL
                     EMAAGTWHAVLSLDTGGIIFEVKHGGYQPVAADDYAHWAPAEGEPGTTELMAWYAQAQ
                     VGDSAFAV"
     gene            complement(1380..1631)
                     /locus_tag="ACIE68_004950"
                     /pseudo
     CDS             complement(1380..1631)
                     /locus_tag="ACIE68_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
BASE COUNT          426 a          444 c          450 g          366 t
ORIGIN      
        1 aagctaataa aaaacacacg tggaatttag gttagactat aaatagaaaa aggcgttttg
       61 acagactatt catgttgttg ttaattcgtc tcttccagaa taaggaatcc catggttaaa
      121 aaatcactgc gtcagttcac gctgatggcg acggcaaccg tcacgctgtt gttaggaagt
      181 gtgccgctgt atgcgcaaac ggcggacgta cagcaaaaac ttgccgaatt agagcggcag
      241 tcgggaggaa gactgggtgt ggcattgatt aacacagcag ataattcgca aatactttat
      301 cgtgctgatg agcgctttgc gatgtgcagc accagtaaag tgatggccgt ggccgcggtg
      361 ctgaagaaaa gtgaaagcga accgaatctg ttaaatcagc gagttgagat caaaaaatct
      421 gacttggtta actataatcc gattgcggaa aagcacgtcg atgggacgat gtcactggct
      481 gagcttagcg cggccgcgct acagtacagc gataacgtgg cgatgaataa gctgatttct
      541 cacgttggcg gcccggctag cgtcaccgcg ttcgcccgac agctgggaga cgaaacgttc
      601 cgtctcgacc gtaccgagcc gacgttaaac accgccattc cgggcgatcc gcgtgatacc
      661 acttcacctc gggcaatggc gcaaactctg cgtaatctga cgctgggtaa agcattgggt
      721 gacagccaac gggcgcagct ggtgacatgg atgaaaggca ataccaccgg tgcagcgagc
      781 attcaggctg gactgcctgc ttcctgggtt gtgggggata aaaccggcag cggtgactat
      841 ggcaccacca acgatatcgc ggtgatctgg ccaaaagatc gtgcgccgct gattctggtc
      901 acttacttca cccagcctca acctaaggca gaaagccgtc gcgatgtatt agcgtcggcg
      961 gctaaaatcg tcaccaacgg tttgtaatag cggaaacgga atggggaaac tcattccgtt
     1021 tttgtttatc gccttagacg gcaaaagcgc tgtcgcccac ctgcgcttgc gcataccagg
     1081 ccataagctc cgtggttcct ggttctcctt ccgctggcgc ccagtgcgca tagtcatcgg
     1141 cagccacggg ttgatagcca ccgtgtttta cttcaaaaat tatgccaccg gtatccagcg
     1201 acagcacggc atgccaggtt cctgcggcca tctccagcac cgtacaggtt tcccccaata
     1261 tcgcccgatg ggtgacggta ccccgatcgt caaaattcag caccacgaaa cgacccctta
     1321 atggcaacag tagttcgaag gtgtgagggt gtcggtgcgg gcgcacgtag gttcctggtt
     1381 ataataatgg cctcacctgc caccattcct gacggccccc agtagataaa ccacattgca
     1441 catccccagg aaatgcccca taagcccccc gtcagcaccg tgacctgcca gaacggcata
     1501 aagggtaatg gcggaagccg gatacccagc cgccagagaa tacgcagaag aggaggggca
     1561 taattgctcc gccacatctt tttgctgtcc atcagggcaa tggcccgggc ttttttctgc
     1621 tcaaaggtca caggagcacc tccgttccat gatgtaaacg tgacacagct tattgttatt
     1681 gattat
//
LOCUS       DBANUU010000345         1670 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000345 DBANUU010000000
VERSION     DBANUU010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.352"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..95
                     /locus_tag="ACIE68_004951"
     CDS             <1..95
                     /locus_tag="ACIE68_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911092.1"
                     /translation="EVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            301..1584
                     /gene="gltA"
                     /locus_tag="ACIE68_004952"
     CDS             301..1584
                     /gene="gltA"
                     /locus_tag="ACIE68_004952"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFX5911093.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT          432 a          405 c          394 g          439 t
ORIGIN      
        1 atgaagtcgg tatttcacct aagattaact tatgtaacag tgtggaagta ttgaccaatt
       61 cattcgggac agttattagt ggtagacaag tttaataatt cggattgcta agtacttgat
      121 tcgccattta ttcgtcatca atggatcctt tacctgcaag cgcccagagc tctgtaccca
      181 ggttttcccc tctttcacag agcggcgagc caaataacaa acgggtaaag ccaggttgat
      241 gtgcgaaggc aaatttaagt tccggcagtc ttacgtaata aggcgctaag gagaccttaa
      301 atggctgata caaaagcaaa actcaccctc aacggggaca cagctgttga actggatgtg
      361 ctgaaaggca cgctgggtca agatgttatt gatatccgta ctctcggttc aaaaggtgtg
      421 ttcacctttg acccaggctt cacttcaacc gcatcctgcg aatctaaaat tacttttatt
      481 gatggtgatg aaggtatttt gctgcaccgc ggtttcccga tcgatcagct ggcgaccgat
      541 tctaactacc tggaagtttg ttacatcctg ctgaatggtg aaaaaccgac tcaggaacag
      601 tatgacgaat ttaaaactac ggtgacccgt cataccatga tccacgagca gattacccgt
      661 ctgttccacg ctttccgtcg cgactcacat ccaatggcag tcatgtgtgg tattaccggc
      721 gcgctggcgg cgttctatca cgactcgctg gatgtaaaca atcctcgtca tcgtgaaatt
      781 gccgcgttcc gcctgctgtc gaaaatgccg accatggccg cgatgtgtta caagtattcc
      841 attggtcagc catttgttta tccgcgcaac gatctctcct atgccggtaa cttcctgaat
      901 atgatgttct ccacgccgtg cgaaccgtat gaagttaatc cgattctgga acgtgctatg
      961 gaccgtattc tgatcctgca cgctgaccat gaacagaacg cctctacctc caccgtgcgt
     1021 accgctggct cttcgggtgc gaacccgttt gcctgtatcg cagcaggtat tgcttcactg
     1081 tggggacctg cgcacggtgg tgctaacgaa gcggcgctga aaatgctgga agaaattagc
     1141 tccgttaaac acattccgga atttgttcgt cgtgcgaaag ataaaaatga ttctttccgc
     1201 ctgatgggct tcggtcaccg cgtgtacaaa aattacgacc cgcgcgccac cgtaatgcgt
     1261 gaaacctgcc atgaagttct gaaagagctg ggcaccaaag atgacctgct ggaagtggct
     1321 atggagctgg aaaacatcgc gctgaacgac ccgtacttta tcgagaagaa actgtacccg
     1381 aacgtcgatt tctactctgg tatcatcctg aaagcgatgg gtattccgtc ttccatgttc
     1441 accgtcattt tcgcaatggc acgtaccgtt ggctggatcg cccactggag cgaaatgcac
     1501 agtgacggta tgaagattgc ccgtccgcgt cagctgtata caggatatga aaaacgcgac
     1561 tttaaaagcg atatcaagcg ttaatggttg attgctaagt tgtaaatatt ttaacccgcc
     1621 gttcaaatgg cgggttaatt tttatatgcc taaacacaaa aaattgtaaa
//
LOCUS       DBANUU010000346         1669 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000346 DBANUU010000000
VERSION     DBANUU010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.353"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..1334
                     /locus_tag="ACIE68_004953"
     CDS             <1..1334
                     /locus_tag="ACIE68_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFX5911094.1"
                     /translation="LIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEM
                     QLYSRYKSELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEE
                     PVRYKFIFNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVL
                     SVDRKNEPEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQG
                     KRFINGAVIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLE
                     QDLSPSGPLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDE
                     YKKLFALPDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNA
                     LLNILESQFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
BASE COUNT          472 a          346 c          417 g          434 t
ORIGIN      
        1 atctgattga tgtctgcaga ctgaatacac tgcgggggcc ggtctctctt tttttcctga
       61 cgcttgccga gtccggagaa cgaaaaaccg cagtggacaa attattaatg aagcccctgt
      121 accagcagga gatgcagtta tattccagat acaaaagtga actggctgtc tggaaaaaca
      181 aggaagagct tttaaaggct cagaaaaaag cattgttgtc aaaactgaat aaagaactcc
      241 gcaaaggcgc ggatgagtca gaaaccctca gacaactgga agtacttcag aaaaacagtg
      301 cggaagaacc tgtgaggtat aaattcattt ttaatgatgc cacgactgcg gcaattaaga
      361 atcagctttg tggtaaatgg cgttcagtgg gtattatgtc agatgaagcc ggtattatat
      421 ttgatggata taccctgtct gagttgcctt ttattaacaa aatgtgggat ggctctgtat
      481 tgtctgtgga cagaaagaat gaacccgaac agatgattga aaatgccaga atgacgttat
      541 ccctcatggt gcaacccggt ctttttgacc gatatatgga gcgaaaaggt tctgttgcca
      601 gggacagtgg ttttcttgcc cgttgtctta tcagtaaacc tgcaaccacg caggggaaac
      661 gctttattaa cggagctgtg attccgggag gatcattaac ggcatttcat gaacgattaa
      721 tggaactggc cagaggcagt attgaaaaat ccagtgagga tgaacggtat tgtctgcatt
      781 tttcgcctga agcgcaaaaa atatttattg agcactataa tgttctggag caggatttat
      841 ccccttccgg acccttaagc ccgtttcggg gacatgtttc aaaaaaaaca gaaaacattg
      901 caagaattgc agcgttattt cagtatttca gctatggtga agggaaaatc tctgccgata
      961 tcatgacgtc tgctgtcgta ataagttcgt ggtacacaga tgaatataaa aaattatttg
     1021 cgttacctga tgaatctgaa ctgcagcaga aggacgccga ggagctgttc gactggctca
     1081 ttgaggagtg ccgcggagaa tgcccgcccc gtgtcaggaa aaattatatt ctccagtgtg
     1141 gcccgggccg gttcagaaac aggaaaaaac tgaacgcatt gctgaacatt cttgaatctc
     1201 agttcaggct ttctgtcgtg ccggaaggaa aaaccatgta tgttctgctt ccccagatag
     1261 ccagtttaaa gttgtctgat gtctctggta tttttacctc gggttatcac tacaataagc
     1321 tgagagcgaa gtaaccggtg agcagttttt ctccattatg cttcagactt agatgattga
     1381 ttattatggg gatttcatca ccgatattca gatgtatgaa tatcggtgat gaactaaagc
     1441 tcaggagctg cgtggtggtg atgaatcaac aggcatataa ctgatacccg ccggtcagaa
     1501 gttccagcct gcgactttcc tgtctgcgtg cccgcagtcc ggcgttcagg tgcagcgtgt
     1561 gacagaacgc acggagagcg aaacggcagg cagacaggcc gttcagtcag ccctggcacc
     1621 ctgcccggat accagcttcc ctgaaacaac tggcatcagg tgcgcttat
//
LOCUS       DBANUU010000347         1658 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000347 DBANUU010000000
VERSION     DBANUU010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.354"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(407..1231)
                     /locus_tag="ACIE68_004954"
     CDS             complement(407..1231)
                     /locus_tag="ACIE68_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911095.1"
                     /translation="MNRSNEEDIEKAKSLISTGAEIAGAAVGGAIGFFATGPVGVAGA
                     GSLGVIVAKAGAKLLGDVADRTMSAREKVRVGAAAAVAFNKIEKVALSGNEPRKDGFF
                     NSEEGKRSDAEEVLEGTLRRAREEYEEKKILLLGNFYANMVYSPGVSTEEANYYLRLF
                     DSLTYRQLCIMSLIMMKPQYSDFQNLRSHDYRTQNANMQASTVALLQEIYALYNLGLV
                     CVRSAGGNGYVSLLAWHDIIPLGLEFTGLGERFYKLFMGGGSFSLPDITTIAEALR"
BASE COUNT          491 a          364 c          311 g          492 t
ORIGIN      
        1 tatagtaata aacctaaatt aaaattatcc atgtttgatg agtggaaata atgtaggaaa
       61 tgaacctgtt taaaaactcg ttatcataga ttaaccgttc agatactcaa cgaactcaat
      121 aaattggctg agtctggtgt attagctcag acctgatctg acagttaccg gttatgtaaa
      181 catttatcgt tgctgccaat gcttggatta ttggcctcaa ggtattcgct ttacttacaa
      241 gctaattttg aaacagatgt cgcgctattt taggaagaga atctatgcaa aagtctgcta
      301 tgagcgagga acggatgttt atgattgtta tcaacgaggc cccatgtcta acaatctcct
      361 gtgttaatga gcaggggctg gaaactttga atcaaaatgt tcatttttaa cgcaaagctt
      421 ctgcgatagt agtaatatcg ggaagtgaaa agcttcctcc ccccataaaa agtttgtaaa
      481 acctttcacc taaccctgta aattctaatc ctaaagggat gatatcatgc catgccagca
      541 aagatacata accatttccg ccagcactcc taacgcagac taatcccaaa ttatacagag
      601 cataaatttc ttggaggagt gctacagtac ttgcttgcat attggcattt tgggttcgat
      661 agtcatggct tctcagattt tgaaagtcag aatattgtgg tttcatcatg atcaaactca
      721 taatacagag ctgccgatac gtcagtgaat cgaacaaccg gagatagtaa ttcgcttctt
      781 ctgtactcac tcccggagag tagaccatat ttgcataaaa attccctaga agaagaatct
      841 tcttttcctc atactcttca cgcgcccttc ttaacgtccc ctccagaact tcttctgcat
      901 cactgcgttt tccttcttca gagttaaaga aaccatcttt tctgggttca ttacctgaca
      961 gagcgacctt ttcaatttta ttgaatgcaa ctgcagcagc agcaccaacg cggacttttt
     1021 cgcgtgcaga catcgtacga tcagccacat caccgagaag ttttgcacca gctttcgcga
     1081 caatcacccc taaagaacca gccccggcaa ctcccacagg gccagttgca aaaaagccaa
     1141 ttgcaccgcc aactgcagcg ccagctatct ctgcacctgt cgaaatcagc gatttggctt
     1201 tttcaatatc ttcttcgttt gatctattca tagctttatc ttctagggca gaataatgga
     1261 tggttgtaat gaagatacct ttaccacatg aaaatcatta actccacatt acctgatgac
     1321 agtgaactgt tgtccgttga ttaacgcgca ataatgttat tcaaatggat gtttgaccac
     1381 ctagaactaa ccgctgtaat gacatctatt tttggtacag aggggaccaa ccggcgtgtc
     1441 ctctggtccg atatgagcga agagcggaag ttcgcagttg ctcttataac ttataaacat
     1501 atgacttacc tatcgctgtc ttaatcaata gggcccaagt gagtgcgtta ccatactagg
     1561 gctgacttca tggtataaga aaaacctttt tgcaaaaggt aaattttcct tttgcatcaa
     1621 aagatatgat tgcttaaaat ggtttgcaat ttaatagt
//
LOCUS       DBANUU010000348         1658 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000348 DBANUU010000000
VERSION     DBANUU010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.355"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..260
                     /locus_tag="ACIE68_004955"
     CDS             <1..260
                     /locus_tag="ACIE68_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFX5911096.1"
                     /translation="SQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEIK
                     VFVKNPLKDLMVEYCEREGITQAQFIEKIIKDELQRLDILK"
     gene            486..560
                     /gene="tap"
                     /locus_tag="ACIE68_004956"
     CDS             486..560
                     /gene="tap"
                     /locus_tag="ACIE68_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFX5911097.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            553..1410
                     /gene="repA"
                     /locus_tag="ACIE68_004957"
     CDS             553..1410
                     /gene="repA"
                     /locus_tag="ACIE68_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFX5911098.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEVAVASARRSRVEWENRQRKKQGLDALGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT          428 a          422 c          434 g          374 t
ORIGIN      
        1 tgtcgcagac agaaaatgca gtgacttcct cattgagtca aaagcggttt gtgcgcagag
       61 gtaagcctat gactgactct gagaaacaaa tggccgctgt tgcaagaaaa cgtcttacac
      121 acaaagagat aaaagttttt gtcaaaaatc ctctgaaaga tctcatggtt gagtactgcg
      181 agagagaggg gataacacag gctcagttca ttgagaaaat tatcaaagat gaactgcaga
      241 gactggatat actaaagtaa agactttact ttgtgtcgta gcatgctaga ttactgatcg
      301 tttaaggaat tttgtggctg gccacgccat aaggtggcag ggaactggtt ctgatgtgga
      361 tttacaggag ccagaaaagc aaaaaccccg ataatcttcg tcaagtttgg cgactgcgaa
      421 gaagattacc ggggcccatt taaaccgtat agccaacaat tcagctatgc ggggagtata
      481 gtcatatgcc cggaaaagtt caagacttct ttctgtgctc actccttctg cgcattgtaa
      541 gtgcaggatg gtgtgactga tcttcaacaa acgtattacc gccaggtaaa gaacccgaat
      601 ccggtgttta caccccgtaa aggtgccgga acgctgaagt tctgcgaaaa actgatggaa
      661 aaggcggtgg gcttcacctc ccgttttgat ttcgccattc atgtggcgca tgcccgttcg
      721 cgtggtctgc gtcggcgcat gccaccggta ctgcgtcgcc gggccattga tgcgctgctg
      781 caggggctct gttttcacta tgatccgctg gccaaccgcg tccagtgctc catcaccacg
      841 ctggccattg agtgcggact ggcgacagag tccggtgcag gaaaactctc catcactcgt
      901 gccacccgtg ccctgacgtt cctgtcagag ctggggttga ttacctacca gacggaatat
      961 gacccgctta tcgggtgcta cattccgacc gacatcacgt tcacaccggc actgtttgcc
     1021 gctctcgatg tgtctgaggt ggcggtggct tccgcacgcc gcagtcgtgt ggaatgggaa
     1081 aacagacagc gcaagaagca gggactggat gcactgggta tggatgaact gatagcgaaa
     1141 gcctggcgtt ttgtgcgtga gcgtttccgc agttaccaga cagagcttaa gtcccgtgga
     1201 ataaaacgtg cccgtgcgcg tcgtgatgcg aacagggaac gtcaggatat cgtcaccctg
     1261 gtgaaacggc agctgacgcg tgaaatctcg gaagggcgct tcactgccaa tcgtgaggcg
     1321 gtaaaacgcg aagtggaacg tcgtgtgaag gagcgcatga ttctgtcacg taaccgcaat
     1381 tacagccggc tggccacagc ttccccctga aagtgacctc ctctgaataa tccggcccgc
     1441 accggaggca tctgcacgcc tgaagcctgt cggcgaacaa aaaaacagca ccgcatacaa
     1501 aaaacaacct catcatccac cttcaggtgc atccagtccc tcctgttttt gatacaaaac
     1561 acgcctcaca gacgaggaat tttgcttatc cacatttaac tgcaagggac ttccccataa
     1621 ggttacaacc gttcatgtca taaagcgcca tccgccag
//
LOCUS       DBANUU010000349         1652 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000349 DBANUU010000000
VERSION     DBANUU010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.356"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>1652
                     /locus_tag="ACIE68_004958"
     CDS             <1..>1652
                     /locus_tag="ACIE68_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFX5911099.1"
                     /translation="NYYLPKESAYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARV
                     GYSKMLLGVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGK
                     KHRDNTLTMKRFTNGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFL
                     GDKRIEGSVWPKSIRGSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDK
                     ETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSS
                     GEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIG
                     ERPDAEVMAERKEHYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQII
                     MGRHDDEQTLLRVDEAINKTYTRRNGAEMSISRICWDTGGIDPTIVYERSKKHGLFRV
                     IPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVH
                     FPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEA"
BASE COUNT          414 a          404 c          478 g          356 t
ORIGIN      
        1 aattactatc tcccgaaaga atccgcatac caggaagggc gctgggaaac actgcccttt
       61 cagcgggcca tcatgaatgc gatgggcagc gactacatcc gtgaggtgaa tgtggtgaag
      121 tctgcccgtg tcggttattc caaaatgctg ttgggtgttt atgcctactt catagagcat
      181 aagcagcgca acacccttat ctggttgccg acggatggtg atgccgagaa ctttatgaaa
      241 actcacgttg agccgaccat ccgtgatatt ccttcgctgc tggcgctggc cccgtggtat
      301 ggcaaaaagc accgggataa cacgctcacc atgaagcgtt tcaccaatgg tcgtggcttc
      361 tggtgcctgg gcggtaaagc ggcaaaaaac taccgtgaaa agtcggtgga tgtggcgggt
      421 tatgatgaac ttgctgcttt tgatgatgat attgaacagg aaggctctcc gacgttcctg
      481 ggtgacaagc gtattgaagg ctcggtctgg ccaaagtcca tccgtggctc cacgccaaaa
      541 gtgagaggca cctgtcagat tgagcgtgca gccagtgaat ccccgcattt tatgcgtttt
      601 catgttgcct gcccgcattg cggggaggag cagtatctta aatttggcga caaagagacg
      661 ccgtttggcc tcaaatggac gccggatgac ccctccagcg tgttttatct ctgcgagcat
      721 aatgcctgcg tcatccgcca gcaggagctg gactttactg atgcccgtta tatctgcgaa
      781 aagaccggga tctggacccg tgatggcatt ctctggtttt cgtcatccgg tgaagagatt
      841 gagccacctg acagtgtgac ctttcacatc tggacagcgt acagcccgtt caccacctgg
      901 gtgcagattg tcaaagactg gatgaaaacg aaaggggata cgggaaaacg taaaaccttc
      961 gtaaacacca cgctcggtga gacgtgggag gcgaaaattg gcgaacgtcc ggatgctgaa
     1021 gtgatggcag agcggaaaga gcattattca gcgcccgttc ctgaccgtgt ggcttacctg
     1081 accgccggta tcgactccca gctggaccgc tacgaaatgc gcgtatgggg atgggggccg
     1141 ggtgaggaaa gctggctgat tgaccggcag attattatgg gccgccacga cgatgaacag
     1201 acgctgctgc gtgtggatga ggccatcaat aaaacctata cccgccggaa tggtgcagaa
     1261 atgtcgatat cccgtatctg ctgggatact ggcgggattg acccgaccat tgtgtatgaa
     1321 cgctcgaaaa aacatgggct gttccgggtg atccccatta aaggggcatc cgtctacgga
     1381 aagccggtgg ccagcatgcc acgtaagcga aacaaaaacg gggtttacct taccgaaatc
     1441 ggtacggata ccgcgaaaga gcagatttat aaccgcttca cactgacgcc ggaaggggat
     1501 gaaccgcttc ccggtgccgt tcacttcccg aataacccgg atatttttga tctgaccgaa
     1561 gcgcagcagc tgactgctga agagcaggtc gaaaaatggg tggatggcag gaaaaaaata
     1621 ctgtgggaca gcaaaaagcg acgcaatgag gc
//
LOCUS       DBANUU010000350         1603 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000350 DBANUU010000000
VERSION     DBANUU010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.357"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..252
                     /locus_tag="ACIE68_004959"
     CDS             <1..252
                     /locus_tag="ACIE68_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFX5911100.1"
                     /translation="MTVTPASASVVKGKSTTLTVAFQPEGATDKSFRAVSADKTKATV
                     SVSGMTITVNGVAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            268..690
                     /locus_tag="ACIE68_004960"
     CDS             268..690
                     /locus_tag="ACIE68_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFX5911101.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RQVTVEDVIRTGACVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNTPEQTEDAGPAEPVSAGKCSTVS"
     gene            672..1106
                     /locus_tag="ACIE68_004961"
     CDS             672..1106
                     /locus_tag="ACIE68_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFX5911102.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            1099..>1603
                     /locus_tag="ACIE68_004962"
     CDS             1099..>1603
                     /locus_tag="ACIE68_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFX5911103.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMNRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRML"
BASE COUNT          358 a          405 c          504 g          336 t
ORIGIN      
        1 atgaccgtga cgccagccag cgcttccgta gtgaaaggga agagcaccac gctgaccgtg
       61 gcattccagc cggaaggcgc aaccgacaag agcttccgtg cggtgtcagc ggataaaaca
      121 aaagccaccg tgtcggtcag tggtatgacc atcaccgtga atggcgttgc tgcaggtaag
      181 gtcaacattc cggtcgtatc cggtaatggt gagtttgctg cggttgcaga aatcaccgtc
      241 accgccagtt aatccggaga gtcagagatg ttcctgaaaa ccgaatcatt tgaacataac
      301 ggcgtgaccg tcacgctttc tgaactgtca gccctgcagc gtattgagca tctcgccctg
      361 atgaaacggc aggcagaaca ggcggagtca gacagcaacc ggcaggttac tgtggaagac
      421 gtcatcagaa ccggtgcttg tgtggtggcg atgtccctgt ggcataacca tccgaagaag
      481 acgcagatgc cgtccatgaa tgaagccgtt aaacagattg aacaggaagt gcttaccacc
      541 tggcccacag aggcaatttc tcatgctgaa aacgtggtgt accggctgtc cggaatgtat
      601 gagtttgttg tgaataatac ccctgaacag acagaggacg ccgggcctgc agagcctgtt
      661 tctgcgggaa agtgttcgac ggtgagctga gttttgccct gaaactggcg cgtgagatgg
      721 ggcgacccga ctggcgcgcc atgcttgccg ggatgtcatc cacggagtat gccgactggc
      781 accgctttta cagtacccat tattttcatg atgttctgct ggatatgcac ttttccgggc
      841 tgacgtacac cgtactcagc ctgtttttca gcgatccgga tatgcatccg ctggatttca
      901 gtctgctgaa ccggcgcgag gctgacgaag agcctgaaga tgatgtgctg atgcagaaag
      961 cggcagggct tgccggaggc gtccgctttg gcccggacgg gaatgaagtt atccccgctt
     1021 ccccggatgt ggcggacatg acggaggatg acgtaatgct gatgacagta tcagaaggga
     1081 tcgcaggagg agtccggtat ggctgaaccg gtaggcgatc tggtcgttga tttgagtctg
     1141 gatgcggcca gatttgacga gcagatggcc agagtcaggc gtcatttttc cggtacggaa
     1201 agtgatgcga aaaaaacagc ggcagtcgtt gaacagtcga tgaaccggca ggcgctggct
     1261 gcacagaaag cgggaatttc cgtcgggcag tataaagccg ccatgcgtat gctgcctgcg
     1321 cagttcactg acgtggccac gcagcttgca ggcgggcaaa gtccgtggct gatcctgctg
     1381 caacaggggg gtcaggtgaa ggactccttc ggcgggatga tccccatgtt ccgggggctt
     1441 gccggtgcga tcaccctgcc gatggtgggg gctacctcgc tggcggtggc gaccggagcg
     1501 ctggcgtatg cctggtatca gggcaactca accctgtccg atttcaacaa aacgctggtc
     1561 ctttccggca atcaggcggg actgacggca gatcgtatgc tgg
//
LOCUS       DBANUU010000351         1601 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000351 DBANUU010000000
VERSION     DBANUU010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.358"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..549
                     /locus_tag="ACIE68_004963"
     CDS             <1..549
                     /locus_tag="ACIE68_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFX5911104.1"
                     /translation="VKLFTDKVTRGGKMKKWMLAICLMFINGICEAADCFDLAGRDYK
                     IDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQHFNELARYGIKPEHLTTDP
                     CMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLAYASEVYRIYTWIKS
                     SKGIRVPTTKKSLSQINSVQNN"
     gene            complement(845..>1601)
                     /locus_tag="ACIE68_004964"
     CDS             complement(845..>1601)
                     /locus_tag="ACIE68_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFX5911105.1"
                     /translation="ELMQFVPSVFSGDKNGARSERYTYIPTINIINKLRDEGFQPFFA
                     CQSRVCDLGRREYSKHMLRLRREGNINGKDVPEIILLNSHDDSSSYQMIPGIFRFVCT
                     NGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFDKVTENMEAMKEIHLNSDEQHLF
                     GRAALMARYEDENKTPVTPEQIITPRRWEDKQKDLWTTWQRVQENMIKGGLSGRSASG
                     KNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
BASE COUNT          458 a          380 c          333 g          430 t
ORIGIN      
        1 gttaaattat ttacggataa agtcaccaga ggtggaaaaa tgaaaaaatg gatgttagca
       61 atctgcctga tgtttataaa tgggatctgc gaagccgccg attgctttga tcttgcaggt
      121 cgggattaca aaatagaccc ggatttactg agagcgatat catggaaaga atcccgttac
      181 cgggttaatg ccatcggtat taatccggta acgggatatg gcagcggact tatgcaggta
      241 gattcccagc attttaacga actggcccgc tatggaatta agccggaaca tctgacaaca
      301 gatccctgca tgaacattta taccggtgct tattatctgg caatagcctt taaaaaatgg
      361 ggcgtctcct gggaggccgt tggtgcatac aatgccggat tcaggaagac cgaacgccag
      421 aaccagagac gtcttgccta cgcatcagag gtttaccgga tttatacctg gataaagagc
      481 agtaaaggca tccgggttcc gaccacgaag aaatcacttt cccaaattaa cagtgtgcag
      541 aacaattaac attgtataca ttgtcaaata ccggttatgc cgcgacagcg gcatggaagg
      601 ccacacacaa aaatatcata tgagtgataa tgctgactgg ccgaagcccc gcaaagcggg
      661 accccatctt atatataccc ggtaactgcg cagcctcctg ccagactatt tttcgcgggc
      721 acaatattta cagactaccg aaaagccagt tgatgaaatt gaggcatatc agacgacaca
      781 gcaatgccgc aggatgataa tataagtaaa gccccgaaaa tttttcgggg ctttacttat
      841 attatcatga cttccactcc ctgaactgtt cggcaatcat ccataatgct ttgttgattc
      901 ggatatcacc gtcaatgcct gtaatggctc ttgttctggt atttttaccg gaggcacttc
      961 gccccgataa tccacctttt atcatattct cctgaacccg ctgccaggtt gtccagagat
     1021 ctttctgttt atcctcccaa cggcggggag taattatttg ttctggcgtc actggcgttt
     1081 tattttcatc ttcgtacctg gccatcaatg cagctctgcc aaataaatgt tgttcgtcac
     1141 tgttaagatg tatctctttc attgcctcca tattttcagt gactttatca aagacaccga
     1201 gcacttcata cgctccctca ataacctgcc cgacaatatc acctttatgt ggaacgcgga
     1261 tttcgccaaa attattccca cacaccaggc catttgtgca gacaaaacga aaaattccgg
     1321 ggatcatctg ataactggat gaatcatcat gtgaattaag caggataatt tcaggaacat
     1381 ctttcccgtt aatattccct tccctgcgaa gacgtaacat atgtttactg tattcgcggc
     1441 gtcccaaatc gcaaacccga ctctgacagg caaagaacgg ctggaaacct tcatcacgta
     1501 acttattgat gatattgatt gttggaatat acgtataacg ttcactccgg gccccatttt
     1561 tatcaccgga aaagacagac ggcacaaact gcattaattc a
//
LOCUS       DBANUU010000352         1587 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000352 DBANUU010000000
VERSION     DBANUU010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.359"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            593..820
                     /gene="ykgL"
                     /locus_tag="ACIE68_004965"
     CDS             593..820
                     /gene="ykgL"
                     /locus_tag="ACIE68_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HFX5911106.1"
                     /translation="MQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(855..995)
                     /gene="ykgO"
                     /locus_tag="ACIE68_004966"
     CDS             complement(855..995)
                     /gene="ykgO"
                     /locus_tag="ACIE68_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFX5911107.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(995..1258)
                     /gene="ykgM"
                     /locus_tag="ACIE68_004967"
     CDS             complement(995..1258)
                     /gene="ykgM"
                     /locus_tag="ACIE68_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFX5911108.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
BASE COUNT          466 a          300 c          353 g          468 t
ORIGIN      
        1 tggttaagtc aatatttaga aaaactgaaa atattgcttt tttgataaga agaaaaagac
       61 atttggtttc ataaggttat tggcaatata acgttcaatt cttacttgca ttttatattg
      121 gtcaggaact ttgccctgaa gtgttaggaa tagtcttaat gcgctgtgtg atggactagt
      181 aaaaaaacaa gttttaaggg taagggatag aatataaaac tatgatgagc tgtcattgaa
      241 gatgatttgc caatcagtaa gatattttaa tggattaaca tgccgcacaa aatggttaca
      301 ggtgttcatt ccaataagta atggaaaatt gaacgggcgt atgtacgtca tctgttcggt
      361 aagcccccaa gagccagatt aagtgaagtt aaacgaaggc tgccagctgt cacaggaacg
      421 atggcctttt tgtcagggca ctgcattcag ttatctgtgc gcgttactct aactggcact
      481 cgaacgtcgg cgacggggtt accggtggat cagactgttc ctgtggcgta aacgttttta
      541 tgttagtacc aacagattta ccacatttat catcttaaca gtttgagtga aaatgcaggc
      601 tgcgccgcaa agggaggaga ctgagtggcg tgttcagtct aaaagagggt tgatgccagc
      661 atacaggggc gaagctggtc aacaagtcaa cattaagata atggaatatt cggagagaaa
      721 cgtcaggcag ttggcctcaa atgaacagga agaatatatc cccaggaaaa ttaatgttgg
      781 tgttataaac acaccaacat taatccgatc tgattattaa tcagacaaat atcgttaatc
      841 cgtcgaattt tgaatcaacg ttttttctta cgaccctgaa cggccttaaa acgtggatta
      901 gatttacaaa tcacatatag ccgtcctttt cgcttcacaa tctgacagtc tggatggcgt
      961 tcttttgcgg tacgcagaga gttaagaact ttcatcacgc cccctttttc gtgctaacaa
     1021 aacgaccaaa acgttgggtg aatcgtgcaa catttccttc tgatgccact gttctcagct
     1081 tccctgtata gaacgggtgc gatttagaag agacatcaat tgtcacgtat ggatacgtta
     1141 cgccatccag ctcaatctca cggtctgttt tgatagtcga gccgatttta aagtactcat
     1201 caacactggt gtcgtggaac accacagtac gatactcagg atggatattg ggcttcatca
     1261 tttttacctg ttatgttata acataaccat aaagatacat gctcatccgg atcatttcaa
     1321 cactcaatcc ctgtgactct ttgtggatgt ggaattttcg caacggggaa ctctggcatt
     1381 gttagcagcc attggtgacg ctcccataaa cgcctggatg agtgattgat gatggttttg
     1441 ctgagagtat ggatttttcg agaatggagg ttatctccac tttactggcc gatgatttat
     1501 cgcagtatga ttgattacct gacagcgtgt attcctctcc cacggatata ataattactt
     1561 acaaaaaagg gagaggatgc atatttt
//
LOCUS       DBANUU010000353         1577 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000353 DBANUU010000000
VERSION     DBANUU010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.360"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(55..1413)
                     /locus_tag="ACIE68_004968"
     CDS             complement(55..1413)
                     /locus_tag="ACIE68_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFX5911109.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCVLSLAFLSCLIYFVKTVIIIQQIIEER
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFG
                     GAIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKNIAHRDPLTNIFNRNYFFNELTVQSASAQKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
BASE COUNT          518 a          349 c          257 g          453 t
ORIGIN      
        1 agaacctcgc ttaggcctgt gtccatatta cgtgggtagg atcaaaatgc cgctttatgg
       61 tgactcacaa aaattcacca catccctcac aaccacctta ttacgcccgg tctctttcgc
      121 ctcgtaaagt gcgttatcag ccagtcgata gatctcattt gggtttaacg cattttcctg
      181 agtcacaaca gcgccaatac taatcgtcac tttttgtggt atagcgtatt caggattatc
      241 gccagttaaa cgctcaacat tttcccgaat cctttccgct aaagctttcg cacgttcagt
      301 atcgatgtcc gtcagcaaga cgccaaactc ttcgccgccg acgcgcgcta aaaggtcatc
      361 tggtcgtatg cttttcccaa tgatattcac cactgttttt atcacctgat cgccaaccgg
      421 atgcccccag gtgtcgttga cttttttgaa gtggtcgata tccataatca tgacgcaata
      481 aggcgttttt tgggctgatg ctgattgaac tgtcagttca ttaaaaaaat aatttctgtt
      541 aaatatattg gttaagggat cgcgatgtgc aatgttcttt gttactcgta gcgcactgaa
      601 tatatggcac ataaatatta ccataacggt taacttactg actacttcaa tagtgcgact
      661 aatataccaa gtggacacgc tatacctgct cataaataac agagagatat tgtacagtac
      721 ggcacaaaaa gcaatcaccg tcactccatt ccagatatca tagcgtaaac cattaaagta
      781 tagattaacc gaaagcaagg ttaaccacat gaaaatcatt atggtaacat atgaggcttt
      841 ccaaaccacc tgaccatttt cattggttaa ctcggcaatg tgtaaattgt agctctcata
      901 atgactagaa agtatgtgag caactatcgc ccccccaaaa acaaaaaaca aacttatgca
      961 caaagtcatt tttttggaaa ataaattgcg ctgttttgtg ttttcactaa ctttattcac
     1021 caatgccaga aatattaata tgcacaaact catctgacga aacaaataat agattgcaat
     1081 gtcattttgg acaacactgc ttgtcaaacg ctcctcaata atttgctgaa tgataatgac
     1141 ggttttaaca aagtaaatca aacaactcag aaaagccaaa ctaagtacac agtttgaaaa
     1201 ttgcctacgg tcataaatga aatacaactg cataaaaata aatgcaacag tatcgataaa
     1261 aaataaagca atcattacca tcagataggt tgatcgtgga acaacttcct caacgcctgg
     1321 ctgattaaac caactattca ccaaaaccaa agcaagccca aacaataagc tcactaatac
     1381 agtactacta attctcaaat agtctttttc cataaagcta caccaatctg taggttgtag
     1441 atccctttct attattaata gatataagaa ctgtactgtt gtcaatatga aattccgccc
     1501 acaaacaata ccgatgatga tagttgctga atgtaattat tattgcctaa gtgctttata
     1561 cattgtttgc aacaagc
//
LOCUS       DBANUU010000354         1555 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000354 DBANUU010000000
VERSION     DBANUU010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.361"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(63..1454)
                     /gene="narK"
                     /locus_tag="ACIE68_004969"
     CDS             complement(63..1454)
                     /gene="narK"
                     /locus_tag="ACIE68_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFX5911110.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT          466 a          432 c          374 g          283 t
ORIGIN      
        1 ctaaatgacg cataaaaaaa gcgcgacatc atgccgcgca aaggataatc aaatattaag
       61 atttattttt tagaatgccg accatatacc gcccaggtaa tcactacgca ggcgatatag
      121 aaaatcaaaa ataccttcat tgcgccgact ggcgaacccg ttaatgccag cgagctacca
      181 aacgctttcg ggataaagaa gccaccaatc gcgccaatcg cagagatgaa acccagcgcc
      241 gccgccgtgt cggttgccgc ttcacgcatc gcacgttcgt cagaaccccc ttctgctttc
      301 acgcgatcca ttgtcagttt acggaagatc actgaaatca tctggaaagt ggaaccacta
      361 cccagcccag ctgtcaggaa cagcgccagg aagaccgcga agaacgccat gaagcttccg
      421 ccctgcccgt cagtcggtaa ggtcaggaac agcaggccgc tgaaaatcgc catcagaata
      481 aagttcacca gcgtgacacg agttccgccc agacggtcag ataatgcacc acctgcagaa
      541 cgcgccagcg caccaataaa cggcccgaag aaagcgtatt gcagaatctg aacatccggg
      601 aactgcgttt ttgacagcat cgcaaagccc gcggagaagc cgataaagga gccgaaggtt
      661 gccagataca gcaggctcat aatccacaga tgaccccgtt tgagtaccgg caactgctcc
      721 ttgatggagg ctttcgaggt agcaagatcg ttcatgccaa accacgccgc aatggtgaag
      781 atggcaagga acggcaccca tatccaggac gcattcgcca gatacagctc agtcccatcc
      841 ggctgtttga cgccctggct accaaatact gcgaaaatcg acagtgatac caccagcgga
      901 gcaaccaact gcatgacgct gacgcccatg ttgcccagac caccattcag acccagcgcg
      961 ccaccctgct tctgtttcgg aaagaagaag ctgatgtttg ccatactgga agcgaagttc
     1021 gcgccagcaa agccgcacag cagagagatg atgatgaaga cgctataagg cgtggaggta
     1081 tcctgcacgg caaaacccag ccagacacaa ggaataatca gaataccggt gctgaacgcc
     1141 gtccagcgac gaccaccgaa gataggaacc ataaaggagt atggaacacg taataacgcg
     1201 ccagaaaccg aaggcagcgc agtcaacata aatagctgat cggtcgtaaa attaaaaccg
     1261 actttcggta agttcaccgc aacagcgctg aacaacatcc atacgcaaaa cgccagcagc
     1321 agacagggaa cggaaatcca caggttgcgg ctggcaatac gttgaccgcg ttgttgccag
     1381 aacgcaggat cttccggtcg ccaatctgta atgacagctc cagtagccct ttcgggggcg
     1441 gatgagtgac tcatagacac ctctgatact cgtttcgatg tctgccacct tagtgtctgt
     1501 agctaaaggc aatttgatgt aaatcaaacg ataagataac tttatctatc attga
//
LOCUS       DBANUU010000355         1546 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000355 DBANUU010000000
VERSION     DBANUU010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.362"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(918..1394)
                     /locus_tag="ACIE68_004970"
     CDS             complement(918..1394)
                     /locus_tag="ACIE68_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFX5911111.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          351 a          413 c          383 g          399 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgcg
       61 caacgcaacg cccttttggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgtcagc catgattttg cccgatttcg
      181 cgctctccac gttcaacagc tcgatcatgt ttttgccgtc cgttcaccgt taaaacgcct
      241 cagaaatcgg gacaggatgt gggaatgtcg gaccgcgcac gcgcactcag acattcccca
      301 cctggtcagg gcttcacccc gacaccctgc atccgtgcgt aagtgctcaa tgtaacttcc
      361 ggttctcttc cgcttcctcg tcgattcgct cgctacgctc ggtcatcgac ttgcggcgag
      421 cgggatcacc tctcgcatgt tgtcggtgat cccgttgttg ttctttcggg gatgagtcgt
      481 cagatagccc ttcaaacacg ctcagaagct ctcagacgca cgaaacgggt ttaaatgccc
      541 gccagggcat ggaaggcgcg aaaaacgctt acagaggctc tcaggaggtc aggcgcgccg
      601 atacccctaa ccaggggctt tcaggtcgct aggaaccgcg tcagctccct gcaaagccta
      661 tttgcacggt acaggcagct attcggctgg ttggtggtcg tagaccgtcg gtgttacagc
      721 gatgggggtc tggggttctc cccagacggt ttccggttgc gctcaaggcg ctgccggaac
      781 cgtggcgacg cggtcgttct tgggatcggg gagcaaaaac acgcaaggga ttttgaggcg
      841 gcaaaagagc aatgcaggat agacaatcat ggtcaaaacg ttaacacgta gcccctgatt
      901 gtccgcctgc cgctggctca atttgaaaac atggccatga ttcggcgctg aatatcaccg
      961 tctttgttga ccagtgtttt agtgacaaac gctgcatggt ttttcgtagt gttgtatccc
     1021 ttccagactt catgtggatc gagggcatac gcacaaacct taccagcaac ggggattcta
     1081 actaatgcat tttttgattc taagtaattg agccaccgaa taattgtgct tctgctaact
     1141 ccaagttttt cagctaggaa ttgctgatcc gcaacgacag cgccacacgt aggatcgata
     1201 tgctccgcta aaaatgagta cagcttcagt gctgatatgc cttggctatc cttcagcagc
     1261 tctcgaacgc gttcccaacc ttttggggat acctgtgtaa accttgaatt cttctttgct
     1321 tcttcgagat cctcggtgtt tttctttgcc tcatctgaaa gttgtttcag tcgtttttct
     1381 gaatcaataa acatgatttt ttccctctga aaatcccatg cactaacgtt ggttcaaatc
     1441 taagccatca tgacacatct gtgcaacacc ttgttgcgtg ttgtcgcatc ctggatgcga
     1501 caacgttgtg tcaccctgga tgcgacaggt aaacaaaaaa cacact
//
LOCUS       DBANUU010000356         1541 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000356 DBANUU010000000
VERSION     DBANUU010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.363"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..132
                     /locus_tag="ACIE68_004971"
     CDS             <1..132
                     /locus_tag="ACIE68_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFX5911112.1"
                     /translation="NRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
     gene            144..647
                     /locus_tag="ACIE68_004972"
     CDS             144..647
                     /locus_tag="ACIE68_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFX5911113.1"
                     /translation="MVVSAIASTPQLTINRKPQGIFSKSPATPLQDKTNTVHNEKPLR
                     VRNQTPWRHMTKRQRKNRKRINRLVEHWPELFNREKTQPLKVEIPDDLIQDIAIRELA
                     FGAGALRAAVASYVQSPRYYRALMAGGARYDQKGQPCGEVTPQEQKEAETRLMVLNDR
                     QKARKPR"
     gene            complement(666..1214)
                     /locus_tag="ACIE68_004973"
     CDS             complement(666..1214)
                     /locus_tag="ACIE68_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911114.1"
                     /translation="MQITEALISEPGDIRRFVQQAVDHWPHLLAFYFTLHSAEGNING
                     QQIHAFCTSFYRQVHERITERNHMASPASPLVLRWLREQHGGATIRCLLLLSQTSICH
                     PRASVTVDEQCSQVVDLLQHSWQVISAGGQCRVERCFRVARGDTSGQYVALKTAALSL
                     GLPVVTAITHRPVQRCTLITAQ"
BASE COUNT          476 a          385 c          346 g          334 t
ORIGIN      
        1 aaccgggatg aagccatctc agtaatacgg gaatacattg agattttcta caatcgtcag
       61 cgtcgtcact ctcgtctggg gaatatctcc ccggcagcct tcagggaaaa atatcatcag
      121 atggctgctt aaaaaaagaa caaatggtag tgtccgctat tgccagtaca cctcagctga
      181 ccattaaccg taaaccgcag ggtattttca gtaagtcacc ggcaacgccg ctgcaggata
      241 aaacgaacac agtacacaac gagaagcctc ttcgggtgag gaatcaaaca ccatggcgac
      301 atatgacaaa acgccagcgc aaaaaccgta agcgtattaa tcgtcttgtg gaacactggc
      361 ctgagttatt caaccgggaa aaaacccaac cgcttaaagt tgagatacct gacgacctga
      421 tacaggatat cgccatcaga gaactggcat ttggcgcagg tgcattacgc gcggcagtag
      481 cgtcttatgt gcagtctccg cgctattacc gtgcgttaat ggctggcggc gcccgttacg
      541 accagaaggg gcagccgtgc ggggaagtga ctccacagga acagaaagag gcagagaccc
      601 ggctaatggt gctgaatgac cgacaaaaag ccagaaaacc tcgttgacta cacaactgcg
      661 ctgattcact gagctgtaat caatgtacag cgctgtaccg gacgatgggt aatggcggtc
      721 acaaccggta accccagaga caatgcggca gtttttaacg ctacatactg accggatgta
      781 tcaccccggg caacccgaaa acacctttcc acccggcatt gtccgccagc acttatcacc
      841 tgccagctat gttgcagtaa atccaccact tgcgaacatt gttcatcaac tgtgacactg
      901 gctcgcgggt gacaaatact cgtctggctg agcagcaaca ggcatcgaat tgttgctcct
      961 ccatgttgtt cccgcaacca gcgtaatacc agcggcgatg ccggactggc catgtgatta
     1021 cgctcagtaa tacgttcgtg cacctgtcgg taaaaggaag tacagaatgc atgaatctgt
     1081 tgcccgttga tatttccttc tgccgaatgg agtgtgaagt aaaatgccag caaatgcggc
     1141 cagtggtcca ccgcctgctg aacaaaacgc cggatgtctc ccggctctga aattaaggct
     1201 tcggttattt gcacgaaatg ttccttttca aaccctgttg gattttgtca agccggtaca
     1261 gacacagtaa agcgcgcatc aacacctgaa aggtacgata caaacacaag ttacaaacaa
     1321 taagaacgga aggacaatta atcagtgagg aataataact aaattaacca aaagaatgtt
     1381 actgacaata ccaccaatac ttaaatagca atatattaat taatgtaaga acagatgccc
     1441 ggtaagacat atacacaata catccacaaa cacactcttt tcattaaata aaccacctga
     1501 ttaaaccatg aaagtgtgat gtaagtcaaa attactgaca c
//
LOCUS       DBANUU010000357         1537 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000357 DBANUU010000000
VERSION     DBANUU010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.364"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            321..998
                     /locus_tag="ACIE68_004974"
     CDS             321..998
                     /locus_tag="ACIE68_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HFX5911115.1"
                     /translation="MEENNTSTDKNTLTALIDYENCGSLKNISLEQYGELIIFVGPQQ
                     NVVVLPADSFPEGARITIRQVSGVSRNNVDFHLVLELGRISCCAAGKDKTYHIISSDK
                     GYDGVIRTLKEAGITCIRRDVPESIAAAGESGADKSNCLKQPSVPESDIVSRWAGKLQ
                     QIAGSGAGNMPVSVETFNNYLKSHMRDEWNKTRAVAIRNELVRRGVTEVREKKIIWKK
                     AKKENKA"
BASE COUNT          475 a          284 c          389 g          389 t
ORIGIN      
        1 acactgccac cggcgcagcc ggggaagggt gtaacgaaaa aagcagcctt cattactcca
       61 gggattcaga aggctgaagt atgcatgaga tttttctttt ttatatctgg cctggcagta
      121 tgaccagtca gcattcatta tcacaggatt attgctgtat taagtaatgg cgtgctgttt
      181 tgtgattgat ttcataaaac aagacgtcgt ttaatatgcg cctgaatatt cacgagggaa
      241 gtctttcttt ttatatggtg ttcccggaaa taaaatattt tgtgttattg ctcacccggt
      301 tatgaataaa aggtaaaaac atggaagaaa ataatacgag tacagataaa aacaccctga
      361 cagcccttat tgattatgaa aactgtggca gcctgaaaaa tatttccctt gagcagtatg
      421 gggaactgat tatttttgtc gggccacagc agaacgtcgt tgttcttcct gcagactctt
      481 ttcctgaagg tgcgagaatc actatcaggc aggtgagtgg tgtgtccagg aataatgtcg
      541 attttcatct tgttctggag ctggggcgga taagctgttg tgcagcaggg aaagataaaa
      601 cataccacat catttcatcg gataagggat atgacggtgt tatcaggacg ctgaaagaag
      661 ccggtattac ctgcatccgt cgtgatgtac cggaaagtat tgctgctgcc ggtgagtctg
      721 gtgcagacaa aagtaactgc ctgaaacaac cttctgttcc tgaaagtgat atcgtcagcc
      781 gttgggcagg aaagctgcag cagatagccg gcagcggagc cggtaatatg cctgtgtccg
      841 tcgaaacatt taataattat ttaaagtcac acatgcgtga cgaatggaac aaaacccggg
      901 cagtggcgat aaggaatgaa ctggttcgtc ggggagtcac agaagtcagg gagaaaaaga
      961 taatctggaa aaaggcgaaa aaggagaata aagcatgagg tatcgcagct gtactcagat
     1021 aaaaacagcc cccgttgcgg ttgcggagcg aaggggctga aaaggggaat taaggatgat
     1081 tgttattatg gtgaaccaga aggttatccc tgtctgtaag ctgaatcgga ctaaacgaca
     1141 taatacctgt aacaatacct gtaacatatt ttcctggcag gacaagcagg aacaataaaa
     1201 tgtgtgattt aattcaaaaa atgtgcgcgg caaagaaaaa ccggggaatt gcttccccgg
     1261 tccggtccat ttagccagtt ccctgagcag gatctgatat gagcattatt cactttgcca
     1321 gacatggtga aaaaagtgaa tatatggagg agaaaaacat cgcctgaatc acagattcgg
     1381 gtgctcagcg cttaagaaga cgggaaagaa aagcgccttt ccgtttcagc atcgggaata
     1441 atcactgaaa catcagcagt atatgtggtg tgtatgaata tcccggcagc attaattctt
     1501 ctgtgaggaa atgcattgat aagaaacaga ataagtc
//
LOCUS       DBANUU010000358         1535 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000358 DBANUU010000000
VERSION     DBANUU010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.365"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..320
                     /locus_tag="ACIE68_004975"
     CDS             <1..320
                     /locus_tag="ACIE68_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFX5911116.1"
                     /translation="HHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAENING
                     LYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGNDD
                     LAA"
     gene            complement(361..1082)
                     /locus_tag="ACIE68_004976"
                     /pseudo
     CDS             complement(361..1082)
                     /locus_tag="ACIE68_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
     gene            complement(1077..1398)
                     /locus_tag="ACIE68_004977"
                     /pseudo
     CDS             complement(1077..1398)
                     /locus_tag="ACIE68_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          389 a          369 c          356 g          421 t
ORIGIN      
        1 tccatcacag tgataaaggt tctcagtatg tatcgctggc ctacacacag cggcttaagg
       61 aagccggatt actggcatca acaggaagta caggcgactc gtatgacaac gcgatggcgg
      121 agaacatcaa tggtctttac aaagcggagg taatacaccg taagagctgg aaaaaccgtg
      181 cagaagtgga actggccaca ctcacgtggg tggactggta taacaatcga cgattgctgg
      241 aaaggctggg ccatactcct ccggcagaag cagaaaaagc ttattatgct tccatcggaa
      301 acgatgatct ggcagcctga gttcacagat aaaacactct ccaggaaacc cggggcggtt
      361 cagaacgacc tcccccttat ttccggtgaa aagaaggttt tttcccgcat tcaggtcatt
      421 tccttttttg ccgtgcatat cccagcttgt atttccctgg gtcagggtgc cggtaccatt
      481 agcagcatca tatttccact gcagtgcttc cgtaccagat gtgtaggtta tggttttgtc
      541 aaaatcattt tgcggctgtt gaccgaggaa gtcctgagtg gtgacgaccc agttattgcc
      601 tgaagaggtc attgaagatg taacaccaat caacaccatt tttttcccca ggaatcataa
      661 gcaaatagtg gagagccact atcccccata attagatagt ttgctaaagg cccctgcgat
      721 gggttaaaaa tattagcgct tggaatggta atcatctgcc cgttattata ggagtttagt
      781 gcaccaactg taccaccgac caaaaaaaca ccatttgtat aaatctgtgt gaggttaccg
      841 tttttgtcct tgataaactg ctgtccgcca ccaagacgat agaatgccgt aaaacggctt
      901 tctgcctgat aggctccgct caccgccccc acatcagaca cttcagtcgg agcaacctct
      961 gtcaccagtt tactcaggcg acggtttttt atatcaagac cactattatt gtttgtgcca
     1021 actgcagtat aagtgtttct ggcgtacccg aagctcatcg tatcggagcc gttaacatgt
     1081 ttttcaataa aaacatactt cattacaggc gcttcgcgaa gtatgtcgct gccttttgga
     1141 ggatggccag ctcctctacc tgctccgcca gttgccgctt caggcgggca ttttcagcgg
     1201 ccagctcact ttcacgctat ggcgaggcta tttattgctg cagtttgctg cgccacgcat
     1261 agagctgaga ttcatacagg ctgagttcac ggactgcggc agcaatacca atgcgttcag
     1321 caagcttcag ggcttcactg cgaaattcag gcgaatgctg tttacgtgtt ttttgctggt
     1381 ttatactgtt tttgtcatgt gagtcacctt tgactgaaag tttactcact tagccgcgtg
     1441 tccactattg cttggtaaga tcacgtctag atttttatca atgagttgct gtacgattga
     1501 ctgataagat taccgcacta atcatcagtg tggta
//
LOCUS       DBANUU010000359         1519 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000359 DBANUU010000000
VERSION     DBANUU010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.366"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..791)
                     /locus_tag="ACIE68_004978"
     CDS             complement(<1..791)
                     /locus_tag="ACIE68_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFX5911117.1"
                     /translation="MPFSNSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHRLTLQLGERRMTLVDLPGIGETPQH
                     DQEYRALYRQLLPELDLIIWILRSDERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     MFPAEEWNATEKCPSRHQELSLATVTARVATLFPSSFPVLPVAAPAGWNLPALVSLMI
                     HALPPQATSAVYSHIRGENRSEQARKHAQQTFGDAIGKSFDDAVAR"
     gene            892..>1519
                     /locus_tag="ACIE68_004979"
     CDS             892..>1519
                     /locus_tag="ACIE68_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911118.1"
                     /translation="MAKNKGICRNKKCSNPVSEFDEANALRLCDDHYKLWKRNKENRR
                     VRNICRIISKYDKSLDREIFYGVRDAAKNKTGKRIIEDAKILHQCRASKQVRKSFHKG
                     VLRSREWLNTQELLSRKHISVTGELFKFYIKDIIRLYCYKFLYSREYLNENAYKKRYQ
                     SDIHPRKVNVPILQLEVAWINNNCRSSDSSTDIIILPRMIRRMVVFNYT"
BASE COUNT          454 a          296 c          386 g          383 t
ORIGIN      
        1 cgggcaacgg cgtcgtcaaa acttttcccg atggcatcac caaaagtctg ttgtgcgtgt
       61 ttccgggcct gttcagagcg gttttccccc ctgatatgtg aataaactgc gctggttgcc
      121 tgtggtggca gcgcgtggat catcagtgac accagcgctg gaaggttcca gcctgcaggc
      181 gcggctacag ggagtaccgg aaatgatgaa gggaacaggg tggccacccg ggctgttact
      241 gtcgccagtg agagttcctg gtgacgggac gggcattttt ctgtggcatt ccattcttca
      301 gcaggaaaca tgcgatcggc atggctgaga acaaacagaa agcgcgaggg atctgcgccc
      361 tcattcagta aaaactgatg catggcaata tcggcagcat acgcacgttc atcactccgc
      421 aggatccaga taatcagatc cagttccggc agtaactgac gataaagcgc tcggtattcc
      481 tgatcatgct gcggtgtttc accaatgccg ggcagatcga ccagcgtcat tctgcgttca
      541 ccgagctgca gggtaagacg atgagcctgg cgggtgcagc cgttcagggg atgcgtggcg
      601 cagatacggg actgaaaaat ggcattacac aggctgctct tgccggtgcc ggatttaccc
      661 ataatgccaa tcacgggctc gtaatgaata acaccgtgga gatgttcaag gattttctgg
      721 cgtatttctt caggaagaaa cgtcagatat gatttcactt gcgcggagag ggaagaattg
      781 ctgaatggca taccggtatc ctgttgattt cactgaaccg gatcacacta atccggttta
      841 tcattcaggt aatatgggtg tgaaaatttt tccattgacg ggaagaagag tatggctaaa
      901 aacaaaggta tttgtcggaa taaaaaatgc agtaatcctg tttcagaatt tgatgaggca
      961 aatgccttaa ggttatgcga tgatcattat aagctatgga agcgaaataa agagaatcgt
     1021 cgggtcagga atatttgtcg tataatcagt aaatatgaca aaagtctgga ccgggagata
     1081 ttttatggag taagagatgc tgcaaaaaat aaaacgggaa agagaattat tgaggacgca
     1141 aaaatattac atcaatgccg tgcctcaaag caagtaagaa aaagttttca caagggtgtg
     1201 cttcggtcac gggaatggct taatacacag gagttactga gcagaaaaca tatatctgtt
     1261 acaggtgaac tatttaaatt ttacataaaa gatattatca gattatattg ctataagttt
     1321 ctctacagca gagaatatct taatgaaaac gcatacaaaa aacgatatca gagtgatatt
     1381 caccccagaa aagttaatgt tccgatactg caacttgaag ttgcatggat aaataataat
     1441 tgccgttcaa gtgattcaag cactgatatt attatccttc cgaggatgat aagaagaatg
     1501 gttgtattta attatacga
//
LOCUS       DBANUU010000360         1507 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000360 DBANUU010000000
VERSION     DBANUU010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.367"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..99
                     /locus_tag="ACIE68_004980"
     CDS             <1..99
                     /locus_tag="ACIE68_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFX5911119.1"
                     /translation="PGTRAVMFSQPASQLLAGARMDVYVIRDGEGN"
     gene            99..1502
                     /gene="traB"
                     /locus_tag="ACIE68_004981"
     CDS             99..1502
                     /gene="traB"
                     /locus_tag="ACIE68_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFX5911120.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKTMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGS"
BASE COUNT          346 a          384 c          484 g          293 t
ORIGIN      
        1 ccgggaaccc gtgccgtgat gttcagccag cctgccagcc agttactggc aggtgcgcgc
       61 atggatgtgt atgtcatccg tgacggggag ggcaactgat ggccagtatc aataccattg
      121 tgaaacgcaa gcagtacctg tggctgggga ttgtggttgt cggtacagcc tccgcgattg
      181 gtggggcact gtatctgtct gatgtggaca tgtccggtaa cggtgaaacc gtggctgaac
      241 aggagcctgt gccggatatg accggtgtgg tggatacgac ctttgatgac aaggtgcgtc
      301 agcatgccac caccgagatg caggtgacgg cagcgcagat gcagaagcag tatgaggaaa
      361 tccgtcgtga gctggatgtt ctgaacaaac agcgcggtga tgaccagcgt cgtattgaaa
      421 agttgggaca ggacaatgcc gccctggcag agcaggtaaa agccctgggt gctaatcccg
      481 tcacggcgac cggtgagcct gtaccgcaga tgcctgcctc accgcccggc ccggaaggcg
      541 aaccacagcc aggaaacacc cccgtatcct tcccgccgca gggcagcgtt gctgttccac
      601 cgccgacggc gttttatccc gggaatggtg tcacgccacc accacaggtg acgtaccagt
      661 ctgtgccggt gcctaaccgg atacagcgta aggtgtttac ccgtaatgag ggaaaacagg
      721 gaccatcact gccgtacatt ccgtcaggaa gttttgcgaa aaccatgctg attgaagggg
      781 cggatgccaa tgcctctgtc accggtaatg aatccacggt gccgatgcag ctgcgtatca
      841 ccggcctggt ggaaatgccg aacagcaaga cgtatgacgc aacgggatgt tttgtgggtc
      901 tggaagcctg gggggatgtg tccagtgagc gtgccattgt acgcacccgc aatatcagtt
      961 gcctgaagga cggcaaaact attgatatgc cgattaaggg gcatgtcagc ttccggggta
     1021 aaaacggtat caagggcgaa gtggtgatgc gtaacggcaa aatcctcggc tgggcatggg
     1081 gcgcgggatt tgttgacggt attggtcagg gaatggagcg cgcctcccag ccggctgtcg
     1141 ggctgggtgc cacagccgct tacggggctg gtgatgtcct gaaaatgggt atcggtggcg
     1201 gcgcatcgaa agccgcacag acgctcagtg actactacat caaacgtgcc gaacagtatc
     1261 acccggtgat accgattggt gcgggcaacg aagtgaccgt ggtgttccag gacggcttcc
     1321 agctgaaaac cgtggaagag atggcgctgg aacgcacgca gagcagagcg gaagaagaca
     1381 atccggaaag tccggttcct gttccgccat cggctgaaag tcatcttaac ggctttaata
     1441 ctgaccagat gctgaagcag ctgggcaacc tgaatccgca gcagtttatg tccggcagct
     1501 agggagg
//
LOCUS       DBANUU010000361         1506 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000361 DBANUU010000000
VERSION     DBANUU010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.368"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..1443
                     /locus_tag="ACIE68_004982"
     CDS             <1..1443
                     /locus_tag="ACIE68_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin family protein"
                     /protein_id="HFX5911121.1"
                     /translation="GATWNIPDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKN
                     LNGQNGTISLRVRPDMAQNNADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQV
                     VEAINGATTEEGAFVQGNRLQAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQ
                     AMDYDRIVAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVR
                     LEGDLMRTEVAGMSVTAGVYGAAGHSSVDVKDDDGSRVGTVRDDAGSLGGYLNLIHNA
                     SGLWADIVALGTRHSMKASTDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTW
                     QGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVS
                     ELPVNWWVQPSVIRTFSSRGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENI
                     TLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
BASE COUNT          334 a          421 c          460 g          291 t
ORIGIN      
        1 ggtgccacct ggaatatccc cgataacgcc acggtgcagt cggtggtgga tgacctcagc
       61 catgccggac agattcattt cacctccacc cgcacaggga agttcgtacc ggcaaccctg
      121 aaagtgaaaa acctgaacgg acagaatggc accatcagcc tgcgtgtacg cccggatatg
      181 gcacagaaca atgctgacag actggtcatt gacggcggca gggcaaccgg aaaaaccatc
      241 ctgaacctgg tgaacgccgg caacagtgcg tcggggctgg cgaccagcgg taagggtatt
      301 caggtggttg aagccattaa cggtgccacc acggaggaag gggcctttgt ccaggggaac
      361 aggctgcagg ccggtgcctt taactactcc ctcaaccggg acagtgatga gagctggtat
      421 ctgcgcagtg aaaatgctta tcgtgcagaa gtccccctgt atgcctccat gctgacacag
      481 gcaatggact atgaccggat tgtggcaggc tcccgcagcc atcagaccgg tgtaaatggt
      541 gaaaacaaca gcgtccgtct cagcattcag ggcggtcatc tcggtcacga taacaatggc
      601 ggtattgccc gtggggccac gccggaaagc agcggcagct atggattcgt ccgtctggag
      661 ggtgacctga tgagaacaga ggttgccggt atgtctgtga ccgcgggggt atatggtgct
      721 gctggccatt cttccgttga tgttaaggat gatgacggct cccgtgtcgg cacggtccgg
      781 gatgatgccg gcagcctggg cggatacctg aatctgatac acaacgcctc cggactgtgg
      841 gctgacattg tggccctggg aacccgccac agcatgaaag cgtcaacgga caataacgac
      901 ttccgcgccc ggggctgggg ctggctgggt tcactggaaa ccggtctgcc cttcagtatc
      961 actgacaacc tgatgctgga gccacaactg cagtatacct ggcagggact ttccctggat
     1021 gacggtaagg acaacgccgg ttatgtgaag ttcgggcatg gcagtgcaca acatgtgcgt
     1081 gccggtttcc gtctgggcag ccacaacgat atgacctttg gcgaaggcac ctcatcccgt
     1141 gcccccctgc gtgacagtgc aaaacacagt gtgagtgaat taccggtgaa ctggtgggta
     1201 cagccttctg ttatccgcac cttcagctcc cggggagata tgcgtgtggg gacttccact
     1261 gcaggcagcg ggatgacgtt ctctccctca cagaatggca catcactgga cctgcaggcc
     1321 ggactggaag cccgtgtccg ggaaaatatc accctgggcg ttcaggccgg ttatgcccac
     1381 agcgtcagcg gcagcagcgc tgaagggtat aacggtcagg ccacactgaa tgtgaccttc
     1441 tgacagaacc atcgcctctc tgtggtcccg gtcatcatga ccgggacccg gaccggcgca
     1501 acggat
//
LOCUS       DBANUU010000362         1506 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000362 DBANUU010000000
VERSION     DBANUU010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.369"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..1443
                     /locus_tag="ACIE68_004983"
     CDS             <1..1443
                     /locus_tag="ACIE68_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFX5911122.1"
                     /translation="GATWNIPDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKN
                     LNGQNGTISLRVRPDMAQNNADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQV
                     VEAINGATTEEGAFIQGNKLQAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQ
                     AMDYDRILAGSRSHQTGVSGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVR
                     LEGDLLRTEVAGMSVTAGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNA
                     SGLWADIVAQGTRHSMKASSDNNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTW
                     QGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVR
                     ELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENI
                     TLGVQAGYVHSVSGSSAEGYNGQATLNVTF"
BASE COUNT          332 a          427 c          459 g          288 t
ORIGIN      
        1 ggtgccacct ggaatattcc cgataacgcc acggtgcagt cggtggtgga tgacctcagc
       61 catgccggac agattcattt cacctccacc cgcacaggga agttcgtacc ggcaaccctg
      121 aaagtgaaaa acctgaacgg acagaatggc accatcagcc tgcgtgtacg cccggatatg
      181 gcacagaaca atgctgacag actggtcatt gacggtggca gggcaaccgg aaaaaccatc
      241 ctgaacctgg tgaacgccgg caacagtgcg tcggggctgg cgaccagcgg taagggtatt
      301 caggtggtgg aagccattaa cggtgccacc acggaggaag gggcctttat ccaggggaat
      361 aagctgcagg ccggtgcctt taactactcc ctcaaccggg acagtgatga gagctggtat
      421 ctgcgcagtg aaaatgctta tcgtgcagaa gtccccctgt atgcctccat gctgacacag
      481 gcaatggact atgaccggat tctggcaggc tcccgcagcc atcagaccgg tgtaagcggt
      541 gaaaataaca gcgtccgtct cagcattcag ggcggtcatc tcggtcacga taacaacggc
      601 ggtattgccc gtggagccac gccggaaagc agcggcagct atggcttcgt ccgtctggag
      661 ggtgacctgc tcagaacaga ggttgccggt atgtctgtga ccgcgggggt atatggtgct
      721 gctggccatt cttccgttga tgttaaggat gatgacggct cccgtgccgg cacggtccgg
      781 gatgatgccg gcagcctggg cggatacctg aatctgatac acaacgcctc cggcctgtgg
      841 gctgacattg tggcccaggg aacccgccac agcatgaaag cctcatcgga caataacgac
      901 ttccgcgtcc ggggctgggg ctggctgggt tcgctggaaa ccggtctgcc cttcagtatc
      961 actgacaatc tgatgctgga gccgcaactg cagtacacct ggcagggact ctccctggat
     1021 gacggccagg ataacgccgg ttatgtgaag ttcgggcatg gcagtgcaca acatgtgcgt
     1081 gccggcttcc gtctgggcag ccaccacgat atgaactttg gtaaaggcac ctcatcccgt
     1141 gacaccctgc gcggcagtgc caaacacagt gtgcgtgaac tgccggtgaa ctggtgggta
     1201 cagccttctg ttatccgcac cttcagctcc cggggggaca tgagcatggg tacagccgca
     1261 gccggcagta acatgacgtt ctcaccgtca cagaatggta cgtcactgga cctgcaggcc
     1321 ggactggaag cccgtgtccg ggaaaatatc accctgggcg ttcaggcagg ttatgtccac
     1381 agcgtcagcg gcagcagcgc tgaaggctat aacggtcagg ccacgctgaa tgtgaccttc
     1441 tgacagaacc atcgcctctc tgtggtcccg gtcatcatga tcgggacccg gaccggcgca
     1501 acggat
//
LOCUS       DBANUU010000363         1495 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000363 DBANUU010000000
VERSION     DBANUU010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.370"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            163..765
                     /locus_tag="ACIE68_004984"
     CDS             163..765
                     /locus_tag="ACIE68_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFX5911123.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKKRRFPRPSHWTREYTHECVEKIKILFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSESEAQSAGRRLVTLEQKLLRMQAQQENISGQ"
     gene            821..1330
                     /locus_tag="ACIE68_004985"
     CDS             821..1330
                     /locus_tag="ACIE68_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911124.1"
                     /translation="MSKSGAEKIQFILNNHEQAYRRLKPVYTFLELASWISVVLLIIY
                     LSGKNHVLTAAFLCFAWTAFWVLFLLHYFPKVFFILDRQSLLNLMEATKDSPDVKQEL
                     LNRLFSRKRMTGRDEMDICRLLRKNEEEKIRQSELNIIRNFIDEQQLTNSDIPKKSTV
                     PNEDKKGIR"
BASE COUNT          483 a          309 c          329 g          374 t
ORIGIN      
        1 cagtagaata tcaattcgac tgtcacgcca taactgtaac tacctcgagt ataaatattc
       61 agagaatgga cttattagcc atgaaaaatc acctgatggg aaatttacta atttatggat
      121 tgtgatatta ttaaagagta aaaccagcca gccgaggtat ttatgggaaa aaatcaggaa
      181 gaaaggaaaa ctcccgtcat tgtggtgaaa aaacgtcgta ctttttcgcc accgtcctta
      241 tccgaaaaaa cagatattat cgcacctgta tttactgaac agacggcaga atcagccccc
      301 gccggaataa attcatctgc cgtcgaaaca catatccccg aagcgccagc caggaagaag
      361 aaaaagaaaa aacgccgttt cccacgtccc tcccactgga cccgtgagta tactcacgag
      421 tgcgtggaaa aaataaagat cctgtttcct catcttcgtg ctgaaggtgg tggatttatt
      481 cccctgaaaa ttggcataaa caatgatatt tcagcatttc tggcagaaca cccggagaca
      541 gaactgacca tggacgaatg gctctgtgca gtgtcctgca tcacctcacg ccgtgtatat
      601 ctgcagcgga cagctgtcgc cggcgtccca cgttatggtc tggatggtca ccccaaaggg
      661 caggtctcag aatctgaagc acagagtgcc ggtcgcagac tggtgacatt agaacaaaag
      721 ttgctcagga tgcaggcaca gcaggagaat atttctgggc aataaacact cctgataaca
      781 gctggacatt acgtatcaga aaaatgggaa gagaattatt atgagtaaat caggtgcaga
      841 aaaaattcaa ttcatcctga ataatcatga acaggcatac agacgcctga agcctgtata
      901 tactttttta gaactggcat cttggatttc ggttgttctg ctgattatat acctatcagg
      961 caagaatcac gtgcttacag cagcttttct ctgctttgca tggacagcat tctgggttct
     1021 tttcctttta cattatttcc caaaagtttt ctttattctc gatcgtcaga gtctgctcaa
     1081 cctcatggag gccaccaaag actcaccaga tgtaaagcag gagctgctta accgcctttt
     1141 ttccagaaaa agaatgaccg ggcgggatga aatggatatc tgccgtcttc tgagaaaaaa
     1201 tgaagaagaa aaaatccgtc agagtgaact gaatatcatc aggaatttta ttgatgaaca
     1261 gcagttaacg aatagtgata ttccgaagaa aagtacggtt ccaaatgaag ataaaaaagg
     1321 cataagatga tatgaaatta ataaccaggc aagcagagga tggcgtggtt tcatcctaag
     1381 attgataggg gttaacctgt aagataaaag gaccgtgttt acacgacgtc tctgaagggc
     1441 ttcgtttggg aaacggacaa tatctaactg acgaggaaat taaataaggg tttaa
//
LOCUS       DBANUU010000364         1490 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000364 DBANUU010000000
VERSION     DBANUU010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.371"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(3..1211)
                     /locus_tag="ACIE68_004986"
     CDS             complement(3..1211)
                     /locus_tag="ACIE68_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFX5911125.1"
                     /translation="MTDGGGMHLLVHPNGSKYWRLQYRYEGKQKMLALGVYPEITLAD
                     ARVRRDETRKLLANGVDPGDKKKNDKVEQSKARTFKEVAIEWHGTNKKWSEDHAHRVL
                     KSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEVAARLQQRTTAIMRYAVQSG
                     LIDYNPAQEMAGAVASCNRQHRPALELKRIPELLTKIDSYTGRPLTRWAIELTLLIFI
                     RSSELRFARWSEIDFEASIWTIPPEREPIPGVKHSHRGSKMRTTHLVPLSTQALAILK
                     QIKQFYGAHDLIFIGDHDSHKPMSENTVNSALRVMGYDTKVEVCGHGFRTMACSSLVE
                     SGLWSRDAVERQMSHMARNSVRAAYIHKAEHLEERRLMLQWWADFLDVNRERFISPFE
                     YAKINNPLKQ"
BASE COUNT          397 a          367 c          301 g          425 t
ORIGIN      
        1 gattactgtt ttaatggatt attaatcttt gcatattcaa atggactgat aaacctttct
       61 ctgtttacat ccagaaaatc ggcccaccac tgtagcatca atcgccgttc ttccagatgc
      121 tctgctttat ggatatacgc ggccctcact gaatttcgcg ccatgtggct catctgacgt
      181 tcaacagcat cacgagacca cagacctgat tcgaccaatg aactacaggc cattgttcga
      241 aagccatgac cacaaacctc tacttttgta tcatacccca tgacccgtaa cgcactattt
      301 accgtattct cactcatggg tttgtgcgaa tcgtgatcac caataaatat caagtcatgg
      361 gccccataaa actgttttat ctgctttaaa attgcaagag cttgcgttga aagaggcact
      421 agatgcgttg tacgcatttt tgagcctcta tgggaatgtt tcactccagg aataggctcc
      481 cgctccggtg ggatagtcca tatagacgct tcgaaatcga tctctgacca acgagcaaaa
      541 cgcagctcac tggaccgaat aaagatcagc aaagtgagtt ctatcgccca tcgggttagc
      601 ggcctaccag tatagctatc tatttttgta agcaactcag ggatgcgctt taattcaagc
      661 gcgggacgat gttgtcgatt acaggaagca accgccccag ccatctcttg tgccgggtta
      721 taatcaatta acccactttg cactgcatag cgcatgatgg ctgtagtgcg ctgctgaaga
      781 cgagcggcca cttcaagacg tccagacatt tctacggcct taataggtgc taataaatct
      841 cgagttttta actcagcgat attacgttca ccaagcgctg caaaaagatt atcttcaaga
      901 ctttttagca cacgatgggc gtgatcttca gaccactttt tattggtgcc atgccactca
      961 atcgcgactt ctttaaaggt tcgtgcttta ctctgttcaa ccttatcatt tttctttttg
     1021 tctcccggat cgacgccatt cgcaagcagc ttacgcgtct cgtcacgacg tactctggca
     1081 tccgctagtg tgatttcagg ataaacccca agtgccagca ttttttgctt tccctcataa
     1141 cggtactgca aacgccagta cttagaacca tttggatgga caagcagatg cataccgcca
     1201 ccatcagtca gctaatattg cttatccgtt ggcttggctg ctctgacttt gatatctgtt
     1261 aatgccatcg tattccctcc ctgttggtac aagcattatc gaaccggaaa taccatcatc
     1321 tgtaccaaca tatgttggta gatgtacgtt gatatcggtt gaccttgaga gagttaatat
     1381 agcgggaaag atagataaac actggatttc aggcataaaa aaagacctca gttgaggtct
     1441 atttacatac ttttggtgcg aaggccggac tcaaacatca aaataagtca
//
LOCUS       DBANUU010000365         1435 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000365 DBANUU010000000
VERSION     DBANUU010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.372"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(14..376)
                     /gene="trbJ"
                     /locus_tag="ACIE68_004987"
     CDS             complement(14..376)
                     /gene="trbJ"
                     /locus_tag="ACIE68_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFX5911126.1"
                     /translation="MLPVLWRGWIPFCRCTEGKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIHQGLFYPASPVEIVVSLCLTFSVSVVFFVVAIVGWISVSEIYYSRRTGRKYRD
                     IPAEMSYAPGEHIKGGQE"
     gene            complement(306..851)
                     /gene="trbB"
                     /locus_tag="ACIE68_004988"
     CDS             complement(306..851)
                     /gene="trbB"
                     /locus_tag="ACIE68_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFX5911127.1"
                     /translation="MSLIKSLLFTLLLAAAAVQASTRDEIERLWNPQGMAAQPAQPAA
                     GTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARMDTVLQMYGGKKGAK"
     gene            complement(838..1122)
                     /gene="traQ"
                     /locus_tag="ACIE68_004989"
     CDS             complement(838..1122)
                     /gene="traQ"
                     /locus_tag="ACIE68_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFX5911128.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMEGQQEGRHVSH"
     gene            complement(1241..>1435)
                     /locus_tag="ACIE68_004990"
     CDS             complement(1241..>1435)
                     /locus_tag="ACIE68_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFX5911129.1"
                     /translation="LTASLWFLCGAIMNQGAAMVVPMVIGSLPNVTSFDGFRRIFICI
                     MLSSVWSGVMWFFIRGLMTG"
BASE COUNT          374 a          391 c          337 g          333 t
ORIGIN      
        1 ttaatgcgtg gcatcattct tgccctccct taatgtgttc tcccggcgca tacgacattt
       61 cagcaggaat atcccgatat ttccgtccag tcctgcggct gtaatatatt tcagacaccg
      121 aaatccatcc cacaattgcc accacaaaaa acacaacaga aacagaaaag gtaaggcaca
      181 gcgaaacgac aatttctaca gggctggcgg gataaaaaag cccctgatgg ataaagggga
      241 tattcaggct gacgattatt cccgtcgcga tgccggatat tccggcaatg agtaacacca
      301 cctgcttatt tcgcaccttt tttccctccg tacatctgca gaacggtatc catccgcgcc
      361 ataaaaccgg cagcatccgt tgccccctgt aaaagcggta atgcctcaag cgtgttgacg
      421 ttgaccagga aggtggtcgg tgtggccacc gggatattcg ggaagaaggt ctgcatcacg
      481 tccggtggca ccggcaatgc ctccggaaag gcggtatccc cctgaccatc cagggtgtag
      541 ggaaaaacgg aaaagccgta ctgctgtgcc agctgtttca gtaccgggtc aaactggtga
      601 cagtaagggc aatgcccctg cataaacagc accaccttcc agtcggccag gttaacctgc
      661 ctgccgttgc tgaggcggaa ccagcggggg gctggctttt ctgtcctggc tgacgtgcct
      721 gctgccggtt gtgcaggctg cgcggccatg ccctgcggat tccagagtcg ttcaatttca
      781 tcccgggtgg aggcctgcac ggcagcggct gccagcaaca gggtgaacaa cagtgattta
      841 atgagagaca tgtctgccct cctgctgccc ttccatcatg gcctgttgcc tggcttccag
      901 tgcctcacgc tcttcccggc tgacacgggc aatccatgca tccagcacct ggtatgcgcc
      961 gaacagcaca aggatggccg caatcaactc cgccagaaaa aaagccatcc agggtttgct
     1021 gtaaacaaga cgggcgacga tatgaaacca gacacccagg gaaaataccc acattcccgt
     1081 aatatcaagc cggggtaaag agaatctgcg tttacttatc atgttaaact ccggttatta
     1141 tttacatgag agtatttatg tctgtaatat tcgcgtttgt gtgatattaa tcagatcaac
     1201 ctcattattt tgtatggttt ttattaccgt tttatatgac ttagcctgtc ataagccccc
     1261 ttataaaaaa ccacatcact ccggaccata ctgatgacaa cataatacag ataaatattc
     1321 ttctgaaccc gtcgaagctc gtaacgttcg gtaatgagcc aataaccatt ggaacaacca
     1381 ttgctgcgcc ctgattcata attgcaccac acaaaaacca gagactggca gtcag
//
LOCUS       DBANUU010000366         1425 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000366 DBANUU010000000
VERSION     DBANUU010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.373"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            11..511
                     /locus_tag="ACIE68_004991"
     CDS             11..511
                     /locus_tag="ACIE68_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5911130.1"
                     /translation="MIRLSLFISLLLTSVAVLADVQINIRGNIYIPPCTINNGQNIVV
                     DFSNVDPGKVSADPQNTSQGKVAKTISISCPYNSGNPWIKVTGRVDNNSLMTDMTNLR
                     IALYQGNNTSTRLTLGEGSGSGYRVTAGLDTTTSTFTFMSVLFRNGDLNGGAFSATAS
                     MSMIYN"
     gene            614..>1425
                     /locus_tag="ACIE68_004992"
     CDS             614..>1425
                     /locus_tag="ACIE68_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFX5911131.1"
                     /translation="MKDIRHILSVAVCLYSSLATGGSAIYFNFKGVQTGEAINNTTSG
                     QTITVPVALNSLGNTGTVAGVPECNGQYDINYYTVFYHRLGIPKKIQLGNQWVDVQVN
                     APGYVRDTEDQNYVYYYSNEENGGRGYRYAGKSCWGAGEVVDVTPFAPGIPPAVTLSV
                     RLPPDLPDGLTEMLIPVKYGKNVNFLRGIWDWNTHRKDLMSAGTTIAAQSTATSFLLY
                     RRDIINKAKCSFDKPSYDIDHKNVSINDALANIAMAETNVKLTCTAPTDVKI"
BASE COUNT          447 a          259 c          336 g          383 t
ORIGIN      
        1 cggaggggga atgattcgtt tatcattatt tatatcgttg cttctgacat cggtcgctgt
       61 actggctgat gtgcagatta acatcagagg aaatatctat attcctccat gcaccattaa
      121 taacggacag aatatcgttg ttgacttcag taatgtcgac ccgggcaagg tgagtgctga
      181 cccacagaat acatctcagg ggaaggtggc aaaaacgata agcatatcct gtccatataa
      241 cagtggaaat ccgtggatca aggtgaccgg aagagtggat aacaattcac ttatgaccga
      301 tatgacaaat ctgagaattg ccttatatca ggggaataac acgtcaacca gactcacatt
      361 aggtgaaggc tcaggaagcg gttacagagt aaccgctggt ctggacacaa caacatcaac
      421 atttactttc atgtcggtgc tgttcagaaa cggagatctg aatggcggtg catttagtgc
      481 tacagccagc atgagtatga tatataacta ggtccttatt atcagagaat gaattatgat
      541 tatcatgggt tcatgccaaa tagcgaaaat atactgagtc tatcagatgt tcggatatta
      601 cagagaggtg tttgtgaagg atattagaca tattttatct gtcgctgtgt gtctttacag
      661 cagtttggct acgggaggga gtgcaattta ttttaacttt aaaggagttc aaacaggaga
      721 ggccattaat aatacgactt ccgggcaaac aatcacagta cctgtggcgt tgaactcttt
      781 aggcaataca ggaactgtag caggtgtccc tgaatgtaat ggacaatatg atataaatta
      841 ttatacggtt ttctaccaca ggttaggtat accaaaaaaa attcaattag ggaatcaatg
      901 ggtcgatgta caggtaaatg cacctggtta tgtcagggat acagaagatc aaaattacgt
      961 gtattattac tccaatgagg aaaatggtgg tcgtggttac agatatgcag gaaagagttg
     1021 ctggggggca ggggaggtcg tagacgtaac tccgtttgcc ccgggtatcc cccctgctgt
     1081 aactctgagt gtacgccttc cgccggactt acccgatggt ttgactgaga tgctgatacc
     1141 ggtcaaatat gggaaaaatg tcaatttttt aagaggcata tgggactgga atactcatcg
     1201 gaaggatttg atgtcggcag ggacaacaat tgcagctcag tccacagcaa catccttcct
     1261 tctgtataga agggatatca taaataaggc aaaatgctca tttgataagc catcatacga
     1321 tatagatcac aaaaacgtta gtatcaacga tgcattagca aatatagcca tggctgaaac
     1381 taatgttaag ttaacatgta ccgcacctac agatgtaaaa attaa
//
LOCUS       DBANUU010000367         1420 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000367 DBANUU010000000
VERSION     DBANUU010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.374"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            87..590
                     /locus_tag="ACIE68_004993"
     CDS             87..590
                     /locus_tag="ACIE68_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HFX5911132.1"
                     /translation="MIMKNLAHIALVTVIQFIACYLADWGSAETVFILCFIVLWQGLF
                     IWLFSQIRKKRHVSDEFKFSKGVWYILMPVSSLLSPLLSLMVFIIGTLFELRRISGCV
                     SVREWMQSQVNEQTNEDLHLDFDDVNFDYVDFYRTNPATGLAMHGGFDSAGNTFGTCW
                     QDYDDRR"
     gene            622..1353
                     /gene="traT"
                     /locus_tag="ACIE68_004994"
     CDS             622..1353
                     /gene="traT"
                     /locus_tag="ACIE68_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFX5911133.1"
                     /translation="MKTKKLMTVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSSSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
BASE COUNT          394 a          265 c          383 g          378 t
ORIGIN      
        1 atgcccgggg ctgacagtcc acaggaactg atgaaaagag caaaggagta tcaggataag
       61 cacaagcaat aatattgtat ggggagatga taatgaaaaa tttagctcat attgctctgg
      121 tgacggtgat acagttcatt gcctgttatc tggctgattg ggggagtgcc gaaacagttt
      181 ttatcctgtg ctttattgta ctatggcagg gattgtttat atggttattt tctcaaatac
      241 gaaaaaaacg gcatgtgtcg gatgagttta aattcagtaa aggcgtttgg tatatactga
      301 tgccagtatc ttccttactc tctccgttat taagcctgat ggtttttatc attggtacac
      361 tattcgaatt aaggcgaatt agtggttgtg taagtgtaag agaatggatg caatctcagg
      421 taaatgaaca aactaatgaa gatttgcatc tggattttga tgatgtgaat tttgattatg
      481 tggattttta tcgtactaat ccggcaacag gattagctat gcatggagga tttgattcag
      541 caggaaatac ttttgggact tgttggcagg actatgatga caggcgataa gaaaactgtg
      601 gtttgtaata aaggtaattt gatgaaaaca aaaaaattga tgacggttgc actggtcagt
      661 tccactctgg ccctttcagg gtgtggtgcg atgagcacag caatcaagaa gcgtaacctt
      721 gaggtgaaga ctcagatgag tgagaccatc tggcttgaac ccgccagcga acgcacggta
      781 tttctgcaga tcaaaaacac gtctgataaa gacatgagtg ggctgcaggg caaaattgct
      841 gatgctgtga aagcaaaagg atatcaggtg gtgacttctc cggataaagc ctactactgg
      901 attcaggcga atgtgctgaa ggccgataag atggatctgc gggagtctca gggatggctg
      961 aaccgtggtt atgagggcgc agcagttggt gcagcgttag gtgccggtat caccggatat
     1021 aattccagtt ctgccggtgc cacactcggt gtgggccttg ctgccggtct ggtgggtatg
     1081 gctgcagatg cgatggtgga agatgtgaac tataccatga tcacggatgt gcagattgca
     1141 gagcgtacta aggcaacggt gacaacggat aatgttgccg ccctgcgtca gggcacatca
     1201 ggtgcgaaaa ttcagaccag tactgaaaca ggtaaccagc ataaatacca gacccgtgtg
     1261 gtctcaaatg cgaacaaggt taacctgaaa tttgaagagg cgaagcctgt tctcgaagac
     1321 caactggcca aatcaatcgc aaatattctc tgattatctt ccggaggctg gtctgaccgg
     1381 cctcctgact gacgcctcgc tttgctcgtt gtccaacccc
//
LOCUS       DBANUU010000368         1405 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000368 DBANUU010000000
VERSION     DBANUU010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.375"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1405)
                     /gene="espL1"
                     /locus_tag="ACIE68_004995"
     CDS             complement(<1..>1405)
                     /gene="espL1"
                     /locus_tag="ACIE68_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFX5911134.1"
                     /translation="NHCMKLSKKIHREKTIQPTVNLNGSAFFLQSPSDAIFCRHLSLQ
                     YALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALLRHPPAGSRVIASKDFGF
                     ALDIFFGRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQLNYVVSVYDPNDTNVAV
                     RGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPLLPEGVPGAIFTGIATRM
                     PFAPIHPSAMLLIMATGQTQQLITLFRQLHILPEKEIIEIITAQNSVGTPALFLAMMN
                     GHTDNVKIFIQEIQSLVDIHIIHEDNLVKLLQTKSANETPGLYISMLYGFDEIIDIFL
                     NALTTPIAQELLNKKLVMSILAMKTHDGEPGLYAAMENNHPLCVTRFLSKINGIAFKY
                     KLSKANIMDLLKGATAQGTPALYIAMSKGNEDVVLSYISTLGAFAKKHSFSQHQLFTL
                     LAAKNHDNMSAVHIAIHH"
BASE COUNT          401 a          272 c          304 g          428 t
ORIGIN      
        1 tatgatgaat ggctatatga acagctgaca tgttgtcatg atttttagca gccaacagtg
       61 taaataactg atgttgacta aaagaatgtt tttttgcaaa agcacccagc gtcgatatat
      121 aagataacac gacgtcttca ttacccttgc tcatggcgat gtataatgca ggggttccct
      181 gtgctgtagc tccttttaat aaatccatga tgttagcttt gctcaacttg tatttaaagg
      241 cgatgccatt aattttagag aggaaccgtg tgacacacaa agggtgatta ttttccattg
      301 cggcgtataa tcctggctca ccatcatgtg ttttcatggc taagatactc atcaccagtt
      361 ttttgtttaa aagctcttgt gctattggag tggttaatgc attcagaaag atatcgatta
      421 tctcatcgaa tccatacaac atggagatat aaagtccagg tgtttcgtta gcacttttag
      481 tttgcagtaa tttaaccaga ttatcttcat gaatgatgtg aatatctacc agtgactgaa
      541 tttcttgtat aaatattttc acgttgtcag tatgtccgtt catcatagcc aaaaataaag
      601 caggtgtacc aacgctattc tgcgcagtta ttatttcaat gatttctttt tcagggagta
      661 tgtgcaactg tctgaataat gtaataagct gttgagtctg gcctgtggcc attattaaca
      721 acattgccga tggatgtata ggggcaaatg gcattcgtgt cgcgataccc gtgaaaatag
      781 cccctggaac tccctcaggt aataggggca gaatagcaat tgcacagttg cgaacatacc
      841 tatcagccca ggtctgagca tcgggacctg aactgatgaa cttatcaagc gagagaaagc
      901 cccgtgctgt cctgtgggtg cctctgacgg caacgttggt atcgttcggg tcgtaaacgg
      961 acacaacata gtttaattgc ccatacgccg actgctttat tcgtagcctt actgacaaag
     1021 tatgattgtc tatatataaa atcgcggaca tatgactgac attgtttgcc atcattcgac
     1081 cgaagaaaat atctaaagcg aagccaaaat ccttactcgc gataacgcga ctccctgcag
     1141 gaggatggcg aagtaatgct ctgaactccg cgtcccggac taaggggaca tgctgctgta
     1201 tggattcaac ggaggagtaa tgtttaatca gattgacttt gccttttcca tttctcagtg
     1261 aatcaagggc gtattgcaat gacagatggc gacaaaaaat agcgtcagaa ggagattgca
     1321 aaaaaaatgc actaccattg agatttactg ttggttgtat agttttttct ctatggattt
     1381 ttttagacag tttcatacaa tggtt
//
LOCUS       DBANUU010000369         1387 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000369 DBANUU010000000
VERSION     DBANUU010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.376"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>1387
                     /locus_tag="ACIE68_004996"
     CDS             <1..>1387
                     /locus_tag="ACIE68_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HFX5911135.1"
                     /translation="SEKLRREVELAEALLKQREQDSDRYSGREDDPQVPRQLRQSRHR
                     RPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLELVSSALKVIRTERVKKAC
                     TKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPLYRQSEIFARQGVELSRAL
                     LSNWVDACCQLMTPVNDALYRYVMNTRKVHTDDTPVKVLAPGQKKAKTGRIWTYVRDD
                     RNVGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAFTGYDRLFSAEREGGALTE
                     VACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEIRGLPESERLAVRQQRSKV
                     LLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYSDDGLAEADNNAAERALRA
                     VCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRHILSVLPEWPSNRVDE
                     LLPWNVVLTNK"
BASE COUNT          341 a          347 c          416 g          283 t
ORIGIN      
        1 gttctgaaaa actgcgtcgt gaagttgaac tggctgaggc tctgctgaaa caacgtgaac
       61 aggacagcga tcgttacagt gggcgggaag acgatcctca ggttccccgc cagttgcgac
      121 agtcgcgcca tcgtcgtccg ttaccggcac accttccccg tgaaatacac cgcctggagc
      181 cagaagaaag ctgttgcccg gagtgtggcg gtgagctgga ttatctgggg gaagtcagcg
      241 ctgaacagct ggaactggtg agcagtgccc tgaaagtgat ccgcacagaa cgggtaaaaa
      301 aagcctgtac aaaatgtgac tgtattgttg aagcaccggc gccgtcccgc ccgatagagc
      361 gtggtatcgc gggccccgga ttacttgccc gcgtgttaac gggaaaatac tgcgaacatc
      421 tgccactgta tcgtcagagt gaaatctttg cccgccaggg tgtcgaactg agccgggcct
      481 tactctccaa ctgggttgac gcgtgctgcc agttaatgac accggtgaat gatgccctgt
      541 accgttatgt aatgaacacc cgcaaggttc acactgatga cacaccggta aaggtactgg
      601 caccgggtca gaaaaaggcg aaaacagggc gtatctggac gtatgtccgg gatgatcgca
      661 atgtgggttc gtcatctcct ccagcggtct ggttcgcgta ctcgccgaac cggcagggga
      721 aacacccgga gcaacacctc cgccccttcc ggggtatcct gcaggcggat gcgttcacag
      781 gttacgacag gttgttcagt gcagaacgtg aaggtggtgc actgacagaa gttgcgtgct
      841 gggcccatgc ccggcgaaaa atccacgatg tatacatcag cagcaaaagt gcgacggcag
      901 aagaagccct gaagcgaatc agtgaactgt acgccatcga ggatgaaata cggggattac
      961 cggagtcaga gcgtcttgcc gtcaggcagc agcgaagcaa agtgttactg acgtcgctgc
     1021 atgaatggat ggtggagaag aatggtacgc tgtcgaaaaa atccagactg ggcgaagcgt
     1081 tcagctatgt actgaatcag tgggatgccc tctgttatta cagtgatgac ggtctggcgg
     1141 aggcggataa taatgctgcg gaaagagcgc ttcgtgcagt ctgtctcgga aagaaaaact
     1201 ttatgttctt tggcagcgat cacggcggcg agcgtggagc actgttgtac gggctgatcg
     1261 gcacctgccg tctgaacggt atcgatccgg aagcgtatct gcgccatatc ctgagcgtac
     1321 tgccggaatg gccttccaac cgagttgatg aactcctgcc atggaacgta gtactcacca
     1381 ataaata
//
LOCUS       DBANUU010000370         1370 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000370 DBANUU010000000
VERSION     DBANUU010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.377"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>1370
                     /locus_tag="ACIE68_004997"
     rRNA            <1..>1370
                     /locus_tag="ACIE68_004997"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          373 a          291 c          439 g          267 t
ORIGIN      
        1 ttgtgagctc gatgagtagg gcgggacacg tggtatcctg tctgaatatg gggggaccat
       61 cctccaaggc taaatactcc tgactgaccg atagtgaacc agtaccgtga gggaaaggcg
      121 aaaagaaccc cggcgagggg agtgaaaaag aacctgaaac cgtgtacgta caagcagtgg
      181 gagcacgctt aggcgtgtga ctgcgtacct tttgtataat gggtcagcga cttatattct
      241 gtagcaaggt taaccgaata ggggagccga agggaaaccg agtcttaact gggcgttaag
      301 ttgcagggta tagacccgaa acccggtgat ctagccatgg gcaggttgaa ggttgggtaa
      361 cactaactgg aggaccgaac cgactaatgt tgaaaaatta gcggatgact tgtggctggg
      421 ggtgaaaggc caatcaaacc gggagatagc tggttctccc cgaaagctat ttaggtagcg
      481 cctcgtgaat tcatctccgg gggtagagca ctgtttcggc aagggggtca tcccgactta
      541 ccaacccgat gcaaactgcg aataccggag aatgttatca cgggagacac acggcgggtg
      601 ctaacgtccg tcgtgaagag ggaaacaacc cagaccgcca gctaaggtcc caaagtcatg
      661 gttaagtggg aaacgatgtg ggaaggccca gacagccagg atgttggctt agaagcagcc
      721 atcatttaaa gaaagcgtaa tagctcactg gtcgagtcgg cctgcgcgga agatgtaacg
      781 gggctaaacc atgcaccgaa gctgcggcag cgacgcttat gcgttgttgg gtaggggagc
      841 gttctgtaag cctgtgaagg tgtgctgtga ggcatgctgg aggtatcaga agtgcgaatg
      901 ctgacataag taacgataaa gcgggtgaaa agcccgctcg ccggaagacc aagggttcct
      961 gtccaacgtt aatcggggca gggtgagtcg acccctaagg cgaggccgaa aggcgtagtc
     1021 gatgggaaac aggttaatat tcctgtactt ggtgttactg cgaagggggg acggagaagg
     1081 ctatgttggc cgggcgacgg ttgtcccggt ttaagcgtgt aggctggttt tccaggcaaa
     1141 tccggaaaat caaggctgag gcgtgatgac gaggcactac ggtgctgaag caacaaatgc
     1201 cctgcttcca ggaaaagcct ctaagcatca ggtaacatca aatcgtaccc caaaccgaca
     1261 caggtggtca ggtagagaat accaaggcgc ttgagagaac tcgggtgaag gaactaggca
     1321 aaatggtgcc gtaacttcgg gagaaggcac gctgatatgt aggtgaagcg
//
LOCUS       DBANUU010000371         1369 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000371 DBANUU010000000
VERSION     DBANUU010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.378"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>1369
                     /locus_tag="ACIE68_004998"
     CDS             <1..>1369
                     /locus_tag="ACIE68_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFX5911136.1"
                     /translation="PEPQTAVEWADANYYLPKESAYQEGRWETLPFQRAIMNAMGSDY
                     VREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDI
                     PSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLGGKAAKNYREKSVDVAGYDELAAFD
                     EDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVRGTCQIERAASESPHFMRFHVACPH
                     CGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGI
                     WTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVN
                     TTLGETWEAKIGERPDAEVMAERKEHYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGP
                     GEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDTGGIDPTIV
                     YERSK"
BASE COUNT          323 a          341 c          406 g          299 t
ORIGIN      
        1 ggccggagcc acagaccgcc gttgaatggg cggatgccaa ttactatctc ccgaaagaat
       61 ccgcatacca ggaagggcgc tgggaaacac tgccctttca gcgggccatc atgaatgcga
      121 tgggcagtga ctacgtccgc gaggtgaatg tggtgaagtc tgcccgtgtt ggttattcca
      181 aaatgctgct gggtgtttat gcctacttca tagagcataa gcagcgcaac acccttatct
      241 ggttgccgac ggatggtgat gccgagaact ttatgaaaac ccacgttgag ccgaccatcc
      301 gcgatattcc gtcgctgctg gcgctggctc cgtggtatgg caaaaagcac cgggataaca
      361 cgctcaccat gaagcgtttt tccaatggtc gtggcttctg gtgcctgggc ggtaaagcgg
      421 caaaaaacta ccgtgaaaag tcggtggatg tggcgggtta tgatgaactt gctgcctttg
      481 atgaggatat tgaacaggaa ggctctccga cgttcctggg cgataagcgt attgaaggct
      541 cggtctggcc aaagtccatc cgtggctcca cgcccaaagt gagaggcaca tgccagattg
      601 agcgtgcagc cagtgaatcc ccgcatttta tgcgttttca tgttgcctgc ccgcactgcg
      661 gggaggagca gtaccttaaa tttggcgata aagagacgcc gtttggcctc aaatggacgc
      721 cggatgaccc ctccagcgtg ttttatctct gcgagcataa tgcctgcgtc atccgtcagc
      781 aggagctgga cttcactgat gcccgttata tctgcgaaaa gaccgggatc tggacccgtg
      841 atggcattct ctggttttcg tcatccggtg aagagattga accgcctgac agtgtgacct
      901 ttcacatctg gacagcgtac agcccgttca ccacctgggt gcagattgtc aaagactgga
      961 tgaagacgaa aggggatacg ggaaaacgta aaaccttcgt gaacaccacg ctcggtgaga
     1021 cgtgggaggc gaaaattggc gaacgtccgg atgctgaagt gatggcagag cggaaagagc
     1081 attattcagc gcccgttcct gaccgtgtgg cttacctgac cgccggtatc gactcccaac
     1141 tggaccgcta cgaaatgcgc gtatggggat gggggccggg tgaggaaagc tggctgattg
     1201 accggcagat tattatgggc cgccacgacg atgaacagac gctgctgcgt gtggatgagg
     1261 ccatcaataa aacctatacc cgccggaatg gtgcagaaat gtcggtatcc cgtatctgct
     1321 gggatactgg cgggattgac ccgaccattg tgtatgaacg ctcgaaaaa
//
LOCUS       DBANUU010000372         1365 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000372 DBANUU010000000
VERSION     DBANUU010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.379"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..350)
                     /locus_tag="ACIE68_004999"
     CDS             complement(<1..350)
                     /locus_tag="ACIE68_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFX5911137.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYA"
     gene            complement(571..1302)
                     /gene="bluR"
                     /locus_tag="ACIE68_005000"
     CDS             complement(571..1302)
                     /gene="bluR"
                     /locus_tag="ACIE68_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFX5911138.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          354 a          313 c          304 g          394 t
ORIGIN      
        1 atggcgtagt aactgcgttt gttatgatta acatcagtaa cccaacggaa aacgcccgcg
       61 cctgctagtg ttgaacagta ttcccgaaat gtagattttc cgcaaatatg aagcaatgcg
      121 gcctctttta ttttagcagg gtttttggtc gtactaactt ttaacaggtt cctggttcct
      181 cttaataaca aaaccgtatc atcgtgagta ataattctga tgttatccgt agccagataa
      241 taaatgtaat gtgcaatacg gtgatgtttt aattctgaat aaaaccagga gaagttttgc
      301 tcttttctca cttgctcaaa catcttttga aaaacaacga cctgatccat caggataata
      361 acctcttgtt agttgtgaga ctgcgtagtg tgcacgatcg gttttaccac ttcaatctgg
      421 tctgtccttt ggctgtgata tgtacagagt gtgatagagg gaatatctga attctcccgg
      481 tgagcatttt gcaacggacc agctccggta caaacgctgt tgtgggttca gattataaca
      541 ttctgtctaa ggggcgggat aaaggtgaaa ttagggggca tgaaagatga ctttataacc
      601 ttgctcaccc caatgttgta aaagttcgtt ttgccttctc gttggtgcca tgcctgtcca
      661 gacaatcaat gtttgcgtcg ggaacagttc cgggcgtggc gattcaatgg gatcagcaag
      721 aacagaaatg tgccatcctg acaatgataa acgccatgct tccagccaca atcgtgctct
      781 ctcttcaaca ttccacgcca tcagaatggc ttctttaccg ggcttacggc gcatttcgaa
      841 aagcgaggct gctgcgtatt caattaatgc gccgtcaaac atactgctca taatgcggga
      901 ggtgttgtga tcaagcacga gacgctggcg aacaggaagg taaacatgat taatcaattg
      961 atctactggg tactctcgac ccagtgaaat aattctcgcg cgtagtttgg caggattagc
     1021 catacgaaga attgacatca tctcttcttg caggcggctc cagtcatctt ccgtatcctg
     1081 gctggtggtt tccagtaatg ctttaacttt gcctacaggg acgccattac ttatccaacg
     1141 cttgatctct tcgatgcgtt gtatgtcttc ttcatcaaag agtcggtgtc cgccttcact
     1201 gcgctgtggt tttaacaaac cgtagcggcg ttgccaggcc cggagagtga caggattaat
     1261 cccgcaacgt tcagcaacat caccaatgct gtaataagcc acaattcctc cttgcggtca
     1321 caaatctccg tcgcctgtac acgacccaat aatactttgt acaat
//
LOCUS       DBANUU010000373         1353 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000373 DBANUU010000000
VERSION     DBANUU010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.380"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..602
                     /gene="traX"
                     /locus_tag="ACIE68_005001"
     CDS             <1..602
                     /gene="traX"
                     /locus_tag="ACIE68_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFX5911139.1"
                     /translation="NRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQSAINRL
                     WGWGIIAQFAYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPL
                     SGTSYGIAGLLMLAVSHRLYRAEDRTERLALVACLLAVIPALNLATSDAAAVAGLVMT
                     VLTVGLVSCAGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            657..1217
                     /locus_tag="ACIE68_005002"
     CDS             657..1217
                     /locus_tag="ACIE68_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFX5911140.1"
                     /translation="MTEQKRPVLTLKRKTEGGTPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAERLAKIRRQNRIKAELQAVLNEK"
BASE COUNT          316 a          338 c          419 g          280 t
ORIGIN      
        1 caaaccgcat tctgcatctg gaccagacgt ggatgtttct ggccgggcgt ggagcctttc
       61 cgttgtttgc tctggtgtgg gggctgaatc tgtcccgcca tgcgcatatc aggcagtcgg
      121 ccattaaccg gctgtgggga tggggaatta ttgcccagtt cgcgtattat ctggcaggct
      181 ttccgtggta tgaggggaat atcctgtttg cctttgcagt ggcggcacag gtgctgacgt
      241 ggtgtgaaac gcgcagtggg tggcgtacag cagcggccat tctcctgatg gcactgtggg
      301 ggcctttgtc cggcaccagt tacggcattg ccgggctgct gatgctggca gtcagccacc
      361 ggctgtaccg ggcggaagac agaacggaac gtctggcgct ggttgcatgc ctgctggctg
      421 ttattccggc gcttaacctt gccaccagtg atgccgcggc ggtggccggc cttgtgatga
      481 cggtgctgac cgttggtctg gtgtcctgtg cagggaaatc attaccccgt ttctggcccg
      541 gagatttttt cccgacgttc tatgcctgtc atctggctgt gctgggcgtt ctggcgctgt
      601 aacgggtgtg gtatctttgg ccgcaagagg atgattcgtc agaggcagaa cacagcatga
      661 cagagcagaa gcgaccggta ctgacactga agcggaaaac agaagggggg acgcctgtcc
      721 gcagccggaa aaccatcatc aatgtcacca cgccaccaaa atggaaggtg aaaaagcaga
      781 agctggccga gaaagcagcc cgggaagcag agctggcggc aaaaaaagcg caggccagac
      841 aagcgctgtc catttatctg aacctgccca cgctggatga ggctgtgaac accctgaagc
      901 cctggtggcc gggattattt gacggtgaca cgccccggct tctggcctgc ggtatccggg
      961 acgtgttact ggaagacgtg gcgcagcgga atatcccgct ctcgcataaa aaactgcgcc
     1021 gggcgctgaa ggccatcacc cgttcagaaa gctatctgtg tgccatgaaa gccggtgcct
     1081 gccggtatga cacggaaggg tatgtaacgg agcatatttc tcaggaagag gaagcgtatg
     1141 cggcagagcg tctggcgaaa attcgccgtc agaaccggat aaaggcagaa cttcaggccg
     1201 tacttaatga gaaataaaaa aagcctcccc gaagaacttc aggaggctga aacaataagg
     1261 cttaaaagga aataacttcc atgcccgaaa ggaaatcggt ccactgaatt atttaccaca
     1321 ataatactga caactaaaag ggaggggcag aaa
//
LOCUS       DBANUU010000374         1346 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000374 DBANUU010000000
VERSION     DBANUU010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.381"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(29..241)
                     /locus_tag="ACIE68_005003"
                     /pseudo
     CDS             complement(29..241)
                     /locus_tag="ACIE68_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            391..1059
                     /locus_tag="ACIE68_005004"
     CDS             391..1059
                     /locus_tag="ACIE68_005004"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFX5911141.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT          399 a          304 c          241 g          402 t
ORIGIN      
        1 attcgaagta aatttataaa atatcaaatc aatatacatt ttgcaccttc gaaatagtaa
       61 tgactgttac tgtaacatga gttacaaaca gcttcatacc cagagctact gatgtatcaa
      121 ctcgattcaa caatacccga tatttattcc atgcctccag caacgatctt tcttcctccg
      181 ttgcgatttc cagatctaca gcatcctgca gtggcgcaat atactcactg aattcctgga
      241 tgtagaactg tgtagtgacg gtcttccagc cattcggctc ctgctgtatc gaagcatacc
      301 aggctatttc aatatcgcta tgctgcggca gcatttaacc ccttgtaatt catcgccata
      361 attgatttaa ttcacaaata aaactataac atggtgaaat taatgaaaaa aaacacagat
      421 gatggggcta aaatttacac accacttacc ctaaagcttt atgactggtg ggttttggga
      481 gtatcaaatc ggcttgcatg gggatgtcct acaaaggaac accttcttcc acactttctg
      541 gaacatttag gtaacaacca tctggatatt ggtgttggaa ctgggtttta ccttactcac
      601 gtacctgaga gtagtctgat atctttaatg gatttgaacg aagctagcct gaacgcggca
      661 tctacaaggg ctggggaatc aaaaattaaa cataaaatta accatgatgt ttttgaacct
      721 tatcccgcgg cgttacatgg tcaatttgat tccatttcca tgttttacct tcttcactgc
      781 ctgcctggaa atatatctac aaaaagctgt gtaatacgca atgcggcgca ggccttaact
      841 gacgatggaa ctctatacgg agccacaatt cttggcgatg gagttgtgca caatagcttc
      901 ggtcaaaaac tgatgcgcat ttacaatcag aaaggcatct tttcaaacac aaaagattcc
      961 gaagaaggct taacacatat actctcagag catttcgaga atgttaaaac caaggttcaa
     1021 ggtactgtag taatgttttc cgcttcaggg aaaaaatagc atccaaccgc agcacgttct
     1081 tgcataaggc gtgtctgcgg tttttcaact attcagatac atcactccca tcacattcat
     1141 tcctccgcat cataggcata taggctatat caccttgata tttttcttct tcagataaaa
     1201 actgttatct atgtatactt ttaaaccgaa tccgtgtaga gtctctacct aagttagttc
     1261 gcagctaccc cttcagtttg cgccattaac ctcctgactt ttgccgctat ctgtaagcat
     1321 tcctgtatac ctgaaactac agggtt
//
LOCUS       DBANUU010000375         1337 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000375 DBANUU010000000
VERSION     DBANUU010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.382"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            1..1219
                     /locus_tag="ACIE68_005005"
                     /pseudo
     CDS             1..1219
                     /locus_tag="ACIE68_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66-like element ISEc43 family transposase"
BASE COUNT          344 a          330 c          376 g          287 t
ORIGIN      
        1 accgcctgga gcctgaagaa agctgttgcc cggagtgtgg cggtgagctt gattatctgg
       61 gggaagtcag cgcagaacaa ctggaactgg tgagcagcgc cctgaaagtg atccgcacag
      121 aacgggtaaa aaaagcctgt acaaaatgtg actgcatcgt tgaagcaccg gcaccatccc
      181 gtccgataga gcgtggtatc gcgggcccgg ggttacttgc ccgcgtgtta acgggaaaat
      241 actgcgaaca cctgccactg tatcgtcaga gtgaaatctt tgcccgccag ggtgtcgaac
      301 tgagccgtgc cttactctcc aactgggttg atgcgtgctg ccagttaatg acgccgctga
      361 atgatgccct gtacagttat gtgatgaaca cccgcaaggt tcacactgat gacacaccag
      421 taaaagtact ggcaccgggc aggaagaagg cgaaaacagg atatatctgg acgtatgtcc
      481 gggatgaccg aaatgccggt tcgccagaac ctccggcggt ctggttcgcc tactcaccgg
      541 accatcaggg taaacatccg gagcaacacc ttcgtccctt ccgggtatcc tgcaggcaga
      601 tgcgttcgca ggttacgatc ggctgttcag tgccgaacgt gaaggcggcg cgttgacgga
      661 agcaggatgc tgggctcatg cgcggcgcaa aatccacgat gtatatatca gtaccaaaag
      721 cgcgacggcg gaagaagcac tgaaactaat cggcgaactg tacgccattg agcacgaaat
      781 acgcgggttg ccggtgtctg aacgcctggc ggtcaggcaa atgcagagta aaccgctact
      841 gacttccctg tataagctga tgcaggagaa agaacacacg ttatcgaaaa aatgccgtct
      901 gagagatgcg ttccggtata tcaggaagca ctgggttgcg ttgtgcaact tctgtgatga
      961 cggtctggcg gaggcggaca ataacacagc ggaaagagcg cttcgtgcag tctgtctcgg
     1021 aaagaaaaat tacgtgttct tcggtagcga tcacggcggc gagcgtggtg cactgttgta
     1081 cgggctgatc ggcacctgcc ggttgaatgg tatcgatccg gaagcgtatc tgcgccatat
     1141 cctgagcgta ctgccggaat ggccttccaa ccgagttgac gaactcctgc catggaacgt
     1201 agtactcact aataaataag cgtcaatacg gtgctccgtt gacgcttact ataggagcgt
     1261 aaattaccta ctatcaaata cttactccaa tagcatttct gtctattcta tttcaattct
     1321 ttaatagaaa aataagg
//
LOCUS       DBANUU010000376         1324 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000376 DBANUU010000000
VERSION     DBANUU010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.383"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            70..1239
                     /locus_tag="ACIE68_005006"
                     /pseudo
     CDS             70..1239
                     /locus_tag="ACIE68_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc52 family transposase"
     gene            complement(1248..1324)
                     /locus_tag="ACIE68_005007"
                     /pseudo
     CDS             complement(1248..1324)
                     /locus_tag="ACIE68_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          367 a          316 c          367 g          274 t
ORIGIN      
        1 agtggtttac tgaatttggc cacctgaaca gaggtgatat gctcacctca gaacaacaca
       61 ggtgccataa tgaaaaaaag aaatttcagc gcagagttta aacgcgaatc cgctcaactg
      121 gtcgttgacc agaattacac cgtggcagat gcagccagcg ctatggatgt cggcctttcc
      181 acaatgacgc gatgggtgaa acaattacgt gatgagcggc agggaaaaac accaaaagcc
      241 tcccctatta ccccggaaca aattgaaatc cgtgagctca ggaaaaagct acaacgtatt
      301 gaaatggaaa atgaaatatt aaaaaaggct accgcgctct tgatgtcaga ctccctgaac
      361 agttctcgat aatcgggaaa ctcagggcgc gttatcctgt ggccactctc tgccatgtgt
      421 tcggggtcca tcgcagcagc tacaaatact ggaaaaaccg tcctgaaaag ccagacggca
      481 gacgggctgt attacgcagc caggtacttg aactgcatgg catcagccac ggctctgccg
      541 gagcaagaag catcgccaca atggcaaccc agagaggcta ccagatgggg cgctggcttg
      601 ctggcagact catgaaagag ctggggctgg tcagctgtca gcagccgact taccggtata
      661 aacgtggtgg tcatgaacat gttgctatcc ctaactacct tgaacggcag ttcgccgtga
      721 ccgagccaaa tcaggtgtgg tgcggtgatg tgacctatat ctggacgggt aagcgctggg
      781 cgtacctcgc cgttgttctc gacctgttcg caagaaaacc agtgggctgg gccatgtcgt
      841 tctcgccgga cagcaggctt accatgaaag cactggaaat ggcatgggaa acccgtggta
      901 agcccgtcgg ggtgatgttc cacagcgatc aaggcagtca ttatacgagc aggcagttcc
      961 ggcagttact gtggagatac cggatcaggc agagtatgag tcggcgtgga aactgctggg
     1021 ataacagccc aatggagcgc ttcttcagga gtctgaagaa cgaatgggtg ccggcgacgg
     1081 gctatgtaag cttcagcgat gcagctcacg caataacgga ctatatcgtt ggatattaca
     1141 gcgcactaag accgcacgaa tataatggtg ggttaccacc aaacgaatcg gaaaatcgat
     1201 actggaaaaa actctaactc ggtggccagt ttttgttgac cacttcaggt tggcaggtta
     1261 cggccaagtt cggtaagagt gagagtttta cagtcaagta aggcgtggca agccaacgtt
     1321 aagc
//
LOCUS       DBANUU010000377         1317 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000377 DBANUU010000000
VERSION     DBANUU010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.384"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            135..794
                     /locus_tag="ACIE68_005008"
     CDS             135..794
                     /locus_tag="ACIE68_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HFX5911142.1"
                     /translation="MGKIITVAGHKGGIGKSTVLCSLCVCVIKKGKTACFLETDSQGS
                     IKDFIEERQANASLSEIPYYECYTDIPAMVQKLAARFDYVFVDTPGMKSPAFVKALSC
                     ADIVLTFVEPGSGIEINTLGRLVFDIKTAQAGVNPSMKAWIVLNKCATNPSDSEASEL
                     RNQLNDDPDWLPVPRQRIYMRTAHKKAYNCGMGVHEYNDKRGNKARGEIELLLKETNI
                     I"
     gene            884..1186
                     /locus_tag="ACIE68_005009"
     CDS             884..1186
                     /locus_tag="ACIE68_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911143.1"
                     /translation="MAKLPKKINQEIKALDLRQFVSSAAQKPASASTVTRINLTMTDT
                     DLDIAQEFQNEYGASRAEVIRTALAALRLISAEERRILFDDIRKNSPKAGRPTVNK"
BASE COUNT          430 a          268 c          285 g          334 t
ORIGIN      
        1 cttctgtcta cacagataat tattgtgtat caatacacac tataaaatta ttgccagcac
       61 gcaattgtcc agatatactc cacacgtaaa taattgtgta ttaatacaca ccattaaacc
      121 aggaggattt tgtgatggga aaaatcatta cagttgctgg gcataaaggc ggcattggca
      181 aaagcaccgt gctttgctcc ttatgtgtgt gtgtcatcaa aaaagggaaa acagcctgtt
      241 tcctggaaac agattcacag ggaagcatca aggatttcat tgaggaacgt caggctaacg
      301 ccagtctgtc ggaaataccg tattacgagt gctacacaga tattccggcg atggtgcaaa
      361 aactggctgc ccgttttgat tatgtgttcg tcgatacgcc tggtatgaaa tctccggcat
      421 tcgtaaaagc actgagttgt gctgatatcg tcctcacatt cgtagaacca ggatccggca
      481 tcgagatcaa cacgctggga cggctggttt tcgacattaa aacagcacag gccggagtca
      541 atccgtcaat gaaagcatgg atcgttctga ataagtgcgc aaccaatccg tcagacagtg
      601 aagcgtcaga actacgcaat caacttaatg atgatcctga ctggcttcct gttccacgtc
      661 agcgcatcta tatgcgcact gcgcataaaa aagcctataa ctgtggtatg ggcgtacatg
      721 aatacaacga caaacgagga aacaaagccc gtggcgagat tgaattactg ttaaaagaaa
      781 cgaatatcat atagtgcgta atattacaca tcgcattaaa aacggaaatt ttcatttata
      841 aataagtgtg tataattaca cactttataa aaaaggaaac tttatggcaa agctccccaa
      901 gaaaataaat caggaaatta aggcgctgga tttacgccag tttgtaagtt ctgccgcaca
      961 aaaaccggca tcagcatcaa cagtaacgcg tataaatttg accatgacag atactgatct
     1021 ggatatagcg caggagttcc agaacgagta cggcgcaagc cgtgctgaag ttatcaggac
     1081 ggcactggca gcgttgcgtc tgatttcagc agaagaacgc cgtatattgt ttgacgatat
     1141 cagaaagaac agcccgaaag caggaagacc aaccgtaaat aaatagcgtg ttttattaca
     1201 cgctattttc gtttgatgaa atgcaaatta tcgctttgaa tgctgcaaca ggagaatata
     1261 actggaagat ggtttctgac catcttaaag ccattattct aaattagata tagatat
//
LOCUS       DBANUU010000378         1311 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000378 DBANUU010000000
VERSION     DBANUU010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.385"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..617
                     /locus_tag="ACIE68_005010"
     CDS             <1..617
                     /locus_tag="ACIE68_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS5-like element IS903 family transposase"
                     /protein_id="HFX5911144.1"
                     /translation="NVSFKTFTRGEIAHLVIDSTGLKVFGEGEWKVKKHGKERRRIWR
                     KLHLAVDSNTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRAASADGAYDTRLCHDEL
                     RRKKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNAQWKWTTDYNRRSIAETAMY
                     RVKQLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
     gene            complement(1082..1291)
                     /locus_tag="ACIE68_005011"
     CDS             complement(1082..1291)
                     /locus_tag="ACIE68_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FaeA/PapI family transcriptional regulator"
                     /protein_id="HFX5911145.1"
                     /translation="MKKKLILNFIQEINAPCQTTEIAIRFDMSAYQARHYLMCLEKEG
                     EIRRTPLRRGVRTLWEAARETEKHQ"
BASE COUNT          376 a          304 c          326 g          305 t
ORIGIN      
        1 ttaatgtcag tttcaaaacg ttcacccggg gtgaaatcgc gcatctggtg attgattcca
       61 ccgggctgaa ggtctttggt gaaggcgaat ggaaagtcaa aaaacacggc aaagaacgcc
      121 gtcgtatatg gcgaaagttg catctggcag ttgacagcaa cacacatgaa atcatctgtg
      181 cagacctgtc gctgaacaat gtgacggact cagaagcctt cccgggtctt atccggcaga
      241 ctcacagaaa aatcagggca gcatcggcag acggcgctta cgacacccgg ctctgtcacg
      301 atgagctgcg gcgtaagaaa atcagcgcgc ttatccctcc ccgaaaaggt gcgggttact
      361 ggcccggtga atatgcagac cgtaaccgtg cagtggctaa tcagcgaatg accgggagta
      421 atgcgcagtg gaaatggaca acagattaca accgtcgctc gatagcggaa acggcgatgt
      481 accgggtaaa acagctgttc gggggttcac tgacgctgcg tgactacgat ggtcaggttg
      541 cggaggctat ggccctggta cgagcgctga acaaaatgac gaaagcaggt atgcctgaaa
      601 gcgtgcgtat tgcctgaaaa aacaacccgc tacgggggag acttacccga aatctgattt
      661 attcaacaaa gcctcgccgg agcaaaatac aaacagctca ttgatattaa caaaaaaact
      721 cagatggaaa accactccgt ggaatatggg atgaccagcc tccatttatt aatacccagt
      781 gatgttagta atatctttat cagcaacctc cggatatccc tgtaaagaag cattttccgg
      841 ggaataactc acccgtattc ttcacgggac cgcaccatgg ggggggaggc aggtttcggc
      901 agattaacac cccatgataa agtagtgcaa ataaagcggt ggaagtagcg atacagtccg
      961 cgtcacagaa acagataatg ccgttgaggg actggtgata agccgcctta ctgttgagtt
     1021 tagtgagtgc cttgatgttc acctttaaga aaagatattt acacaaaatg gtaaacagtt
     1081 gttattgatg cttttccgtt tctctggctg cctcccataa agtacggaca ccacgacgaa
     1141 gaggcgtacg cctgatttct ccctctttct caagacacat aagataatgg cgagcctgat
     1201 aagcactcat atcaaaacgt atagcgattt ctgttgtttg gcatggtgcg tttatctcct
     1261 gaataaaatt cagaatcaac tttttcttca ttcctgttca atccgtatct g
//
LOCUS       DBANUU010000379         1288 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000379 DBANUU010000000
VERSION     DBANUU010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.386"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1243)
                     /locus_tag="ACIE68_005012"
     CDS             complement(<1..1243)
                     /locus_tag="ACIE68_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HFX5911146.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEEIRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMGDLYSHRWEIELGYREIKQTMQRSR
                     LTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLM
                     TLQGASPGRIPELMRDLASMGQ"
BASE COUNT          269 a          364 c          332 g          323 t
ORIGIN      
        1 gttgtcccat acttgcaaga tcgcgcatca gctccggtat acgtcccggt gaagcgccct
       61 gcaatgtcat cagcattctc atcaccattc cgcatgattc tgagaaactc agttgattcg
      121 gccagtaacc tttcagatgt tccgccattt taatcatctg atatctcacc agattataag
      181 ccagtaagac accccacagc tcttgctcca caagctccgg ctttttactt ctcagcgtca
      241 gcctgctccg ttgcatcgtc tgttttatct ccctgtatcc cagttcgatt tcccagcgat
      301 gactgtacag atcccccatt tctcctccgg ggaagcgcat ggcgtccgtc atcgacgtca
      361 gcagatggca gacttttcct ttgcgcgtca cggtcagcag gcgggcagtc acttcatttc
      421 ccagtcccgg ccactttttt cgtgcctgcg ggctggtttt cagcttcacc agatgatcgc
      481 ctttacccag ttttctgatc tcttcatatt gcgctccctt tctgagaggt atcatccagt
      541 ggcggtgttc tcccgccagg ctccaggcat ttaacagtcc cagtgagtaa taacctttat
      601 ccattaacgt cagagtgtta tcgccggttt gttctataag ttgctcagca agctcatttt
      661 cgctgttctt catcgtgccg aaggctgcag ccgtcagcag atggctggtc agttccatct
      721 ggcagaccat tttgacctgc gggtagagcg ccgggttccc ggcatgtgtc tggcggggga
      781 aggctgcatc gttctctggt gtatccggtg tgcgccagaa cacaccatcg atggccagca
      841 gggtcaggcc gcaccagtgc ggatgcggcg tggcgttatg ccagagctgc gctgttttcg
      901 tgaacacgcg gcggacagcc tcacttccca ggcgctggcg ggcctgaata acggcactgg
      961 gggcaacgaa ggggcgattg cccggcagca tgatgtccag gcgattcaca atctggtgaa
     1021 gaggttcttt acgctcaagc gccatgccaa caatacacca gaccatcatt tcgaggggaa
     1081 gacggcgctt gcgtagcgtt acagtacctg attcggcaag gcaacgagag atgagttcgg
     1141 ggtcgaggta atcccccaga gaagtcagtg ggttacgcag agaatcgtaa cgggatacca
     1201 gatcaagagc ctgtccaatg tgcataaaaa aatccggaaa caagtgagcg tttccggatt
     1261 cttacacagc cactggatcg gtcaactg
//
LOCUS       DBANUU010000380         1277 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000380 DBANUU010000000
VERSION     DBANUU010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.387"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            129..887
                     /locus_tag="ACIE68_005013"
     CDS             129..887
                     /locus_tag="ACIE68_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFX5911147.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(940..1047)
                     /gene="yniD"
                     /locus_tag="ACIE68_005014"
     CDS             complement(940..1047)
                     /gene="yniD"
                     /locus_tag="ACIE68_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFX5911148.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
BASE COUNT          321 a          290 c          303 g          363 t
ORIGIN      
        1 gaatacattg ctaagttatt cgcgcctttt tataattcgt tacctttcca gggacgctaa
       61 tctttgatcc ttaagaaagt ttccgggacc gcgatggtct ctttttttgt tgtacggact
      121 cttgataaat gaagcttttg aagacagttc ctgccatcgt tatgttggcg gggggcatgt
      181 ttgcgtcact gaatgcagct gccgatgatt ccgtttttac tgtcatggac gatcctgcca
      241 gcgctaaaaa acctttcgaa ggtaatctga acgcaggata tcttgcacaa tcaggcaata
      301 cgaaaagctc ctcacttacg gctgacacca ccatgacctg gtatggacaa accactgcct
      361 ggtcgctgtg ggggaacgcc agtaatactt cttctaatga tgagcgttct tcagaaaaat
      421 atgcggcggg cgggcgtagc cgttttaatt taactgatta tgactattta tttggtcagg
      481 caagctggct gacagaccgt tataacggct atcgagagcg cgatgtgttg accgcgggtt
      541 atggtcgtca gtttttgaat ggcccggtac acagtttccg ctttgaattc ggtccaggtg
      601 tgcgttacga caaatatacc gacaatgcca gtgaaaccca gccgctgggc tatgcctccg
      661 gggcttatgc atggcagttg actgacaacg ctaaatttac gcagggtgtt tcggtctttg
      721 gcgcggaaga cacaacgctg aactccgaaa gcgcattaaa tgttgctatt aatgaacatt
      781 ttggactgaa ggtggcttac aacgtcacat ggaactctga acctccagag tctgcaccgg
      841 aacataccga ccgtcggaca accttatcgc tgggctacag catgtaaaat tatctgccgg
      901 accgtgttat gcggtctggc tgtcttcgtt tgtcactgct cagccccaaa tcattgctgc
      961 ccaacgtagc aacaacatag cgccacagat tgccagtagc accaccacca tcttccagtg
     1021 ttttttatca aagcgtttag tcggcatacc agtgtcctgc atatctcttg tgcgtgataa
     1081 cggagtgtaa caaaagggac aggcatcaag ggattttttg ttattacagc tgtcgggtgg
     1141 cgttgtgcac atggtcactg tttctgcgtt ttatgaaaaa aataaaatta tgtatcgata
     1201 tacaaatatc ataaatagct ggttataaat acgtctttag ttcatcagca ctaaaactca
     1261 ggctttggct gattgca
//
LOCUS       DBANUU010000381         1261 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000381 DBANUU010000000
VERSION     DBANUU010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.388"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..685)
                     /locus_tag="ACIE68_005015"
     CDS             complement(<1..685)
                     /locus_tag="ACIE68_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFX5911149.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDEDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKYLDQDI
                     LNILFFGHCI"
     gene            complement(711..>1261)
                     /gene="rfaY"
                     /locus_tag="ACIE68_005016"
     CDS             complement(711..>1261)
                     /gene="rfaY"
                     /locus_tag="ACIE68_005016"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFX5911150.1"
                     /translation="LIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRV
                     RSAGLTFPNDFYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEK
                     LHALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDY
                     GYYSVIYRTKLRKFIKKLKGKA"
BASE COUNT          397 a          262 c          222 g          380 t
ORIGIN      
        1 aaatacaatg accaaagaac agaatattta aaatatcctg atcaaggtat ttatacttcg
       61 tgtgctgcgg gtcacttgcc agcatagcaa tggctttatt cataaaatca tttttcgccc
      121 atgcttcaag ctgcagatat actacaccgg cgttgaaata attccctgga agtccctcta
      181 tagccagacg cttcgctggt ttttcctgca taaaaggcac atctttaata acagctgcga
      241 acttatcctc atcaaaatta atatcaagca atccagctaa tgagccttta cagatgacat
      301 cagcatcgat atataacaga ttttcaatag tttcactcaa atactggaac gcaactaaac
      361 ggaagaatgt tgcataagac cactgtttca ggctggggaa gatatcaata aaggcattgg
      421 agactttata aattattact tcaacgttag catttttgct ggtaaaagca ttaacacgtt
      481 gtagataatc ttcatcaatg tagtcggtaa agagatgaaa cttaaccgca aggtccgggt
      541 tatgcataac aactgattgc atagatactg ccgcaccaaa taaaaagtta cggtcaatac
      601 cgtaagcgat atttaacaca cgatcggtgt tagcaatttc cccagagaca gcgacttcag
      661 taatactctc agctaataaa tccatgttgg ttccgttttg actgtgtggt ttacgctttg
      721 ccttttaatt ttttaataaa tttgcgtagt ttggttctgt agattactga atagtagcca
      781 taatctttta tttcgttggc aatacccagg tgacgttcca tggccaggcg atcacgagct
      841 ttacgctctg ccgtacagct tttaccggat aggtcaatta tcctgacacc atctttacta
      901 acgataaagt taccccggtg tgggtcgccc gatagcatat ttaaggcatg caatttttcc
      961 attgaagcct taatttctgc tttaacattt tctggaataa tcggcatatc attgagctct
     1021 acgccctcga catactcgat aagcatgata aaaacactgg catagttgaa aattttacgc
     1081 tcagccagaa aataaaaatc attaggaaaa gtaagacctg cacttctgac acgatcggtt
     1141 tcaacgatca aattctgata gtagtcacct tttacaaaag attttaaaaa acgttcattc
     1201 cttttcgttt tcggagcaaa gactttaaaa acatagcgac catatttcgt atcaatcaac
     1261 g
//
LOCUS       DBANUU010000382         1237 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000382 DBANUU010000000
VERSION     DBANUU010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.389"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1237)
                     /locus_tag="ACIE68_005017"
     CDS             complement(<1..>1237)
                     /locus_tag="ACIE68_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFX5911151.1"
                     /translation="TIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGESVDAA
                     LLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEEEARA
                     FSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTSPSRL
                     FRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQKWTWI
                     PRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKAMYAA
                     TIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLMPGDS
                     LNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANESWAYF
                     MGRRKFVASRQASQMFLCWL"
BASE COUNT          255 a          397 c          331 g          254 t
ORIGIN      
        1 agccagcaca gaaacatctg gctcgcctga cgggatgcga cgaattttcg ccgccccata
       61 aagtacgccc acgactcgtt cgcactggcc cgtgccgtgg agtagctcat ctgggcgtaa
      121 ttccgggaaa gctgctcata cgagacaccc agcccggcag cgatataccg caacagtgac
      181 tgctcaaaca cggagtagcc gttatccgta tcctgagccg tctgcaggtt cagtgagtcc
      241 cccggcatca ggtgcggcac ttttgcgcct cccagacgga ccggtgctgc ggcgtaatac
      301 gcggcaattt caccaatcca gcccgtcagc ctttcccgct gctcctgact gttcgcgccc
      361 agaataaaat ccatcgctga ctgcgtatcc agctcacttt caatggtggc ggcatacatc
      421 gccttcacaa tggcgctctg cagctgcgtg ttctgcagcg tgtcgagcat cttcatctgc
      481 tccatcacgc tgtaaaacac atttgcaccg cgggtctgcc cgtcctccac gggttcaaaa
      541 acgtgaatga acgaggcgcg cccgccgggt aactcacggg gtatccatgt ccatttctgc
      601 ggcatccagc caggataccc gtcctcgctg acgtaatatc ccagcgccgc accgctgtca
      661 ttaatctgca caccggcacg gcagttccgg ctgtcgccgg tattgttcgg gttgctgatg
      721 cgcttcgggc tgaccatccg gaactgtgtc cggaaaagcc gcgacggact ggtatcccag
      781 gtggcctgaa cgaacagttc accgttaaag gcgtgcatgg ccacaccttc ccgaatcatc
      841 atggtaaacg tgcgttttcg ctcaacgtca atgcagcagc agtcatcctc ggcaaactct
      901 ttccatgccg cttcaacctc gcgggaaaag gcacgggctt cttcctcccc gatgcccaga
      961 tagcgccagc ttgggcgatg actgagccgg aaaaaagacc cgacgatatg atcctgatgc
     1021 aactggatgg cgttggcggc atagccgtta ttgcgtacca gatcgtctgc gcgggcattg
     1081 ccacgggtaa agttgggcag cagggctgca tccacacttt cacccggtgg gttccacgcc
     1141 cgcaactgcc caccaaatcc gctgccaccg ccgtgataac cggcatattc acgcagcgat
     1201 gtcatgccgt ccggccccag aagggtggga atggtgg
//
LOCUS       DBANUU010000383         1232 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000383 DBANUU010000000
VERSION     DBANUU010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.390"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>1232
                     /gene="traD"
                     /locus_tag="ACIE68_005018"
     CDS             <1..>1232
                     /gene="traD"
                     /locus_tag="ACIE68_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFX5911152.1"
                     /translation="GMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSA
                     FVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTENPKDVARMLKKDGKD
                     SDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVIYDRSGEFVK
                     SYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQGSGRTI
                     FAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAV
                     LTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMW
                     LSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQ
                     LEDIYGEKAAATLFDVMNT"
BASE COUNT          292 a          302 c          342 g          296 t
ORIGIN      
        1 ggcatgcggg atttaatcaa gtcccagccg gtctatgaga tccagtatta cggcaaaacc
       61 ttccggatga acgctgctca ggtactgcat gataaatata tgatctggtg cggagagcag
      121 ctatggtccg cattcgttct ggctacagtt gtggcactgg ttatttgcct gattaccttc
      181 tttgttgtct cctggattct ggggcgtcag ggtaaacaac agagcgaaaa tgaagtcaca
      241 ggtggtcgtc agctgacgga gaacccgaaa gacgttgccc ggatgctgaa aaaagacggc
      301 aaggactccg atatacggat tggcgacctg ccgattatcc gggattccga aatccagaac
      361 ttctgtctgc acggcacggt cggggccggt aagtcggaag ttatccgtcg tctggccaac
      421 tacgcccgtc agcgtggaga tatggtggtg atttatgacc gttcagggga atttgttaaa
      481 agttactatg atccctccat cgataaaatc ctgaatccgc tggatgcccg ctgtgccgcc
      541 tgggatttgt ggaaggagtg cctgacacag ccggattttg ataataccgc aaatactctg
      601 atcccgatgg gcacaaaaga ggacccgttc tggcagggtt caggacgtac catttttgcg
      661 gaagcggcgt acctgatgcg taatgacccc aaccgcagct acagcaaact ggtggacaca
      721 ctgctttcca tcaaaatcga aaaactgcgt accttcctgc gtaattcacc ggcggccaac
      781 ctggtggaag agaaaattga gaaaacggcg atttccatcc gtgctgtgct gaccaactac
      841 gtgaaagcca tccgttacct gcaggggatt gagcataacg gtgagtcttt taccatccgt
      901 gactggatgc ggggtgtccg ggaagatcag aaaaacggct ggctgtttat ctcgtcgaat
      961 gccgacaccc atgcctccct gaagccggtg atctccatgt ggctgtccat tgccattcgt
     1021 ggtctgctgg caatgggaga aaaccgtaac cgtcgtgtgt ggtttttctg tgatgagcta
     1081 cccacgttac acaaactgcc ggacctggtg gagatcctgc cggaagcccg taagttcggt
     1141 ggctgttatg tgtttggtat ccagtcctat gcccagctgg aagatatcta cggtgagaaa
     1201 gcggctgcca cgctgtttga cgtcatgaac ac
//
LOCUS       DBANUU010000384         1224 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.391, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000384 DBANUU010000000
VERSION     DBANUU010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.391"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..653
                     /locus_tag="ACIE68_005019"
     CDS             <1..653
                     /locus_tag="ACIE68_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFX5911153.1"
                     /translation="DIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAF
                     EEKFPLKELNNPAHDSSAMSEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAV
                     SFRSIIAEQKKEPEMMVRYYISSADLTAEKFATAIRNHWHVENNLHWRLDVVMNEDDC
                     KIRRGNAAELFSGIRHIAINVLTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            683..1096
                     /locus_tag="ACIE68_005020"
     CDS             683..1096
                     /locus_tag="ACIE68_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911154.1"
                     /translation="MLVGAAVGAVVIGGGIVIYNNREAVGDAIEKSMEMRGEAELLNA
                     QSQMEFWREPYGAFSSVFGNDDSSKENPNVGKDLTDEEKNCLGSGSTGGSGGWEPDED
                     GEIRNTYRSIKDAPQYPRGVRNVQNGTTRNVVKDQ"
BASE COUNT          394 a          184 c          344 g          302 t
ORIGIN      
        1 tggatattaa agggaaaatc atcacaactg atgcgatggg ttgccagaaa gatattgcag
       61 agaagataca aaaacaggga ggtgattatt tattcgcggt aaaaggaaac caggggcggc
      121 taaataaagc ctttgaggaa aaatttccgc tgaaagaatt aaataatcca gcgcatgaca
      181 gttccgcaat gagtgaaaag agtcacggca gagaagaaat ccgtcttcat attgtttgcg
      241 atgtccctga tgaacttatt gatttcacgt ttgaatggaa agggctgaaa aaattatgcg
      301 tggcagtctc ctttcgctca ataatagcag agcaaaagaa agagcccgaa atgatggtca
      361 gatattatat cagttctgct gatttaaccg ctgagaagtt tgcgacagcg attcgaaatc
      421 actggcacgt ggagaataat ctgcactggc gtctggacgt ggtaatgaat gaagacgact
      481 gcaaaataag aagaggaaat gcagcagaat tattttcagg gatacggcac atcgctatta
      541 atgttttgac gaatgataag gtattcaagg cagggttaag acgtaagatg cgaaaagcag
      601 ccatggacag aaactatctg gcgtcagtcc ttgcggggag cgggctttcg taatcttgcc
      661 ctgggtagga ggccctccag ggatgttagt cggtgcggca gttggagcgg tggttattgg
      721 tggtggtata gtaatataca acaatcgcga ggctgttggg gatgcgattg agaaaagtat
      781 ggagatgcgg ggtgaagccg aattattaaa tgcacaaagt caaatggaat tctggcgaga
      841 accctatggt gctttttcca gcgtgtttgg aaatgatgat tcatcaaaag agaatccaaa
      901 tgtcgggaag gatttgacgg atgaagagaa gaactgtttg ggcagtggca gtacaggagg
      961 ttcgggaggg tgggagccag atgaggatgg cgaaataaga aatacctaca ggtctattaa
     1021 ggatgctcct cagtatccca gaggggttag gaatgtccaa aatggcacta caaggaatgt
     1081 tgtaaaggat cagtagttat tgaaacaatt acgtaaagta gattcaggaa aatagagtaa
     1141 agtttataaa gatggatttg atgcgtcagg tcgaaaagtg tccatttact attttgaaag
     1201 tcagagtggt cgagtattta atgt
//
LOCUS       DBANUU010000385         1196 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000385 DBANUU010000000
VERSION     DBANUU010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.392"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            686..1155
                     /locus_tag="ACIE68_005021"
                     /pseudo
     CDS             686..1155
                     /locus_tag="ACIE68_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535762.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT          349 a          225 c          250 g          372 t
ORIGIN      
        1 attattgaca aaactaaata taaataacaa ctgcctcggc atgccagtta acagctatcc
       61 gtatggcgtg ttcggttttg cgaataaaaa atggttactg tagaagcgga tttccaccac
      121 atgtcaacca tgaatggtca gtggtgcgag acgactggga tgatttggtc gatgacttct
      181 tccggcgaat actgatagaa acattgaaat attgtaagag aaagcgttca aaattctgtg
      241 tcacgtgaaa aattctacag cgtcatcacc ttattcagtc gaccttcaaa ataaacgttt
      301 aacagagaca atcaccattt ggattgtccg ctgaggaggt gaacgatttc ctagacagct
      361 ttatcatgcc agcccacatt gttggcgctg acaatactaa ggacaaacga tggggaagta
      421 gctttttagc gttattttcg ggagtaaaaa acgtggtatc tttccgtatg tcagttgtat
      481 cttgctggta ttcgtagtcg tgatagggca tacaacgata tttatcttga cgttagtgaa
      541 gaataagttg ttttgtgatc tatgttaatc tgggattaca atccgggctt gatacatcgc
      601 atcacgactt ttattctctt cagagtagaa tatgttactc cccgttttac aggctcagga
      661 tttgaattac aaattatact tttaagtgaa caataaccag gggtatttct tatttcctga
      721 aaccaggctt ggctcatttc gggatgagtt tgaaaaaact cttgacgcat acgatagtga
      781 tgaaataaac gaaacaaatt acgtcaacaa actcaggcgt cttgcctgac atgctcctga
      841 catcattgat attcacgctt atctggcata tgcatttgcg ctctttatgc tgcaattctg
      901 gcgaatgttc atttacagcg tcatcaggat gccgttatac tcattgataa aatactaaca
      961 tataaccctg agaataatca ggggacacgc tggttactgg ggacggaatt gttacgtgct
     1021 ggcaagcatg acagggctct gtacgtattg acaggacata tcaatgaatt ttccccctac
     1081 tggtacgagc ttgctctttt acattttatg aatgaggcat atattaatgc tgcaacggca
     1141 tttcgatatg gatttttaac aagcatatat tgttgaaatg cttttttaga atcttc
//
LOCUS       DBANUU010000386         1194 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000386 DBANUU010000000
VERSION     DBANUU010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.393"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..308)
                     /locus_tag="ACIE68_005022"
     CDS             complement(<1..308)
                     /locus_tag="ACIE68_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911155.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPTGNNLEALAPGIEKLKQ
                     TSIEMVTLLNTLQPGGKCIITGDFQKELAYLQNVILYNVSSLRLDFLGYNAQIIQRSD
                     "
     gene            complement(330..869)
                     /gene="yhaB"
                     /locus_tag="ACIE68_005023"
     CDS             complement(330..869)
                     /gene="yhaB"
                     /locus_tag="ACIE68_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFX5911156.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYHGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPLVHFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(1125..>1194)
                     /locus_tag="ACIE68_005024"
     CDS             complement(1125..>1194)
                     /locus_tag="ACIE68_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFX5911157.1"
                     /translation="QLINVNFFSSDRTSFCECNRFP"
BASE COUNT          366 a          212 c          224 g          392 t
ORIGIN      
        1 ttgtccgatc gttgaataat ttgggcgtta taacctaaaa aatccagacg aagagacgag
       61 acattataaa gaattacatt ttgtaagtac gctaattctt tttgaaaatc accagtgata
      121 atgcattttc caccaggttg taacgtgtta agtaaagtga ccatttcaat agaggtctgt
      181 tttagttttt ctatacctgg tgctagtgct tcgaggttat ttcctgttgg gctttcagga
      241 aggtcattca tttttcgacg gactaaatca ctactaatat cattaccaat tgatgaaaca
      301 gggaacatta ttttatcctt tataaataat caataaaaat cattattaaa atgatcgggt
      361 gaaaaaaaga gaatttgcca acaatctgaa gtaatgctgg ttttattaaa gtgcacaagg
      421 ggtgttaaga tggcttcttt tcgggtgaat tttgcacaat acttagaaat cgtctcctta
      481 atacattcca gctctttttt attgacggct gagcaagtac attgttcacc aaaaggtgca
      541 aaagtgccta catctaatgt ttgtatgata taatcatcat gttctcctgt agaaatatct
      601 ataatgaatg gactcatatt ttccggttgg attcggatca tgacagaagg aacatcgcca
      661 tgatagaaat ttaatgcagt tattgcagat aatactctcc gcagttcatt acttatactg
      721 taaattttat taacgtcatt ggtggagaca atttctgcaa actttctttt taccgtccaa
      781 taatcaatat ttctattgtg attgttaacc actgcgtgca ttggcgggat tgaagggtga
      841 aaaatatgcg caattgggaa gcctttcatt gatgcctgcc ttgtcatcat gacaaacaat
      901 aatagatgct tattttcctc gcagggttct ttcgcactat caaaaaacgc tcatttaccc
      961 ggcgttacag taaacgaagc aacattctgt ggcctacgcc gatcaaaagc tctgtgaaaa
     1021 tttcccttgt tgtctttgtc tgaccttatc gcggcgatca gcaattattc gttttgtgat
     1081 cctcaacaca aaatgctatt cgtcgcctat gaggggagaa gaatttatgg aaaacgattg
     1141 cattcacaaa atgacgtcct gtcagaagag aaaaagttga cgttaatcaa ttgt
//
LOCUS       DBANUU010000387         1189 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000387 DBANUU010000000
VERSION     DBANUU010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.394"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..1167
                     /gene="ygeH"
                     /locus_tag="ACIE68_005025"
     CDS             <1..1167
                     /gene="ygeH"
                     /locus_tag="ACIE68_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFX5911158.1"
                     /translation="LTRCIYSLRCIFEKIGYDRCIETIYRKGYRFSGQVFKTKINEDN
                     TSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAATNFCNDHISQNS
                     FLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDELHNTSQFIIDNI
                     LQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYDFTPESIYRAMTIFDRLQNKS
                     DIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDITTVDGKILAIMGL
                     ITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVHFHNEKIEEARICIDKSLQLEPR
                     RRKAVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLKQLTRICMR"
BASE COUNT          430 a          214 c          197 g          348 t
ORIGIN      
        1 ctgacaagat gtatctattc tttgcgctgc atttttgaaa aaattggcta tgatcgttgc
       61 atagaaacaa tctaccggaa aggttatcgt ttcagcgggc aggttttcaa aactaaaatt
      121 aatgaagata atacttcaga ctattccata gctatattcc ctttcactac ttcattgaat
      181 acactggatc cattaatact taatcaggaa ttagtgcaaa tcatttcaaa taaaaaaatc
      241 gatggtctct atacctatcc gatggctgcg acaaattttt gtaatgatca catatctcaa
      301 aattcattct tgagcagatt caagccagat tatttcgtta caggaagaat aaaccagaat
      361 aatgcagtga acactttata cattgagttg atcgacgcta aaaacctttt cctcatcgcc
      421 agtaatcatc tccctgttga tgaactacat aatacatcac aatttattat agataatatc
      481 cttcaaacgg tacataaacc agaacgatct gtaagattag ctaagcagga ccaaggatat
      541 aagaatcatt atttatcaga tgaaatgtta gccggaaaga aagaacttta cgacttcacc
      601 cctgaaagca tttacagggc catgactata tttgatagat tacaaaataa aagtgatata
      661 cagacgctaa aaacagaatg ttattgcctt ctagcggaat gccatatgtc tttggcactt
      721 catggaaaaa gtgaacttga acttgctgct caaaaagcat tagagctttt agattatgta
      781 tcagacataa ccactgtcga tggaaaaatt ttagctatta tgggactgat aactggtctg
      841 tctggacaag caaaagtatc tcatatctta tttgaacagg ctaagataca ctcaactgat
      901 atagcctctc tctactacta tagggcacta gtccactttc ataatgaaaa aattgaagag
      961 gcaaggattt gtatagacaa atcactacaa ctcgaaccca gaagacgaaa agcagttgtg
     1021 ataaaagaat gtgtagatat gtatgtgcct aacccgctca aaaacaacat taaactctac
     1081 tataaagaaa ctgagagtga aagccatcga gttataattg acaacatttt gaaattaaag
     1141 cagctgacga gaatttgtat gcgataaatt tttattgacg caattttat
//
LOCUS       DBANUU010000388         1172 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000388 DBANUU010000000
VERSION     DBANUU010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.395"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..483)
                     /gene="sfmF"
                     /locus_tag="ACIE68_005026"
     CDS             complement(<1..483)
                     /gene="sfmF"
                     /locus_tag="ACIE68_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFX5911159.1"
                     /translation="MRRALFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     K"
     gene            complement(494..>1172)
                     /gene="fimH"
                     /locus_tag="ACIE68_005027"
     CDS             complement(494..>1172)
                     /gene="fimH"
                     /locus_tag="ACIE68_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFX5911160.1"
                     /translation="VDSVMDIQFPPQNYILMGTDPNVSQNLPFGVMDSRLIFRLKVIR
                     PFINMVEIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFG
                     DIGASLFSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQ
                     DLGFIVADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALG
                     YLRVDYQ"
BASE COUNT          320 a          313 c          267 g          272 t
ORIGIN      
        1 tttcgccaca cctggccgaa caccgctggc taacgctcga taattggcaa aaaagtttag
       61 tgtgacattg ccatttgcat ctacttcctc agtcgggctc gcctccccca gtgcgagccg
      121 ggagcgatcg ctattacgta attcgatggc gacggtttgt gccattgcgg gatcatccag
      181 agccagcagg ttggtatcgg atgccggcgt tcccgtaaat aaaatcgcaa ctgaacccgg
      241 aggacatccc tccagccgca ggctaaaagg gacgagtgcc gtggtatcgc cagcgttcag
      301 taattgtgtc gtaggccatc tgcctaaatc taccgtctta tcaatatccg cagtgtttac
      361 ggtacaggag aaatcaacaa cgttaccgtg caaattgata ttaatcgttc ccaaagggtc
      421 agctgcccat ccactggaac tccacagtaa cccgcagaaa cagctaaaga gtgctcttct
      481 cattctccgt acctcattga taatcgacgc gtaaataccc cagcgcgcta aacggccctt
      541 cggtcggttt ttgaccggta atactgatag gccaggcgcg aagtgtgaca ttggctgccg
      601 cagctgcatc cagacggaaa ggaataacgc tattgagatc gttaggcgtg atcggcgtat
      661 cgttctgatc ggcgacaata aaacctaaat cctgattgtc cgacaccatc gcctgaccag
      721 aaacggcact ggcttccaga cgcattgtta aataagcctg cgcagcaaca ttcgtacatt
      781 tgaccgcaat gctcttggtt tgcggcatga caccagcagg tcgattaccc ggccctgccg
      841 cactaaataa cgatgcgccg atatcaccaa aatcaaattc aacaatctgc ccggcattta
      901 attcgcagtt ttgcggtact tcaacccggc caccaaaact aatggtataa acaggtgtaa
      961 ccaacggatc gtaaggcgtt gatgtcacat acacggtaaa catcacctgt ctggggatct
     1021 ccaccatgtt aataaaggga cgaataacct ttaaacgaaa tattaaacga gaatccatca
     1081 ccccgaatgg aaggttttgc gaaacgttag gatctgttcc catcagaata taattttgcg
     1141 gggggaactg aatatccatc acactatcaa cc
//
LOCUS       DBANUU010000389         1159 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000389 DBANUU010000000
VERSION     DBANUU010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.396"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>1159
                     /locus_tag="ACIE68_005028"
     CDS             <1..>1159
                     /locus_tag="ACIE68_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFX5911161.1"
                     /translation="DYTWNDNGQLVRISGPQECREYRYSGTGRLTGVHTTAANLDIDI
                     PYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAEKTDRIPEGVI
                     RMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGW
                     MSLSRKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKARHRS
                     LAEVLQEDTGVTLPAELAVMLGRLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAE
                     YIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGEESAQHLQQSLRLPGQ
                     QYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLG"
BASE COUNT          272 a          306 c          406 g          175 t
ORIGIN      
        1 gtgactatac gtggaacgat aacggacaac tggtgcgcat cagcggcccg caggagtgcc
       61 gggagtaccg ttacagcggc acgggaaggc tgacgggcgt ccacactacg gcagcgaacc
      121 tggatatcga tatcccgtat gcgacggacc cggcaggaaa ccggctgccg gacccggaac
      181 tgcacccgga cagcaccctc acggcgtggc cggataaccg catcgcggaa gatgcgcact
      241 atgtctaccg ctacgatgaa tacggcaggc tggcggagaa gacggaccgc atcccggaag
      301 gggtgatacg gatgcacgac gagcggaccc accactacca ctacgacagc cagcaccgtc
      361 tggtgttcta cacgcggata cagcacggtg agccgcaggt ggagagccgc tacctctatg
      421 acccgctggg ccgccggacg ggaaaacggg tgtggcggcg ggagcgtgac ctgacggggt
      481 ggatgtcgct gtcgcgtaaa ccggaggaga cctggtacgg gtgggacggt gacaggctga
      541 ccactgtaca gacccaacag acaagaatcc agacggtata ccagccggga agcttcacgc
      601 cgctcctgag aatcgaaaca gagaatggtg aacaggcgaa ggcgcggcac cgtagcctgg
      661 cggaggtgtt gcaggaggac acgggtgtga cgctaccggc ggagctggcg gtgatgctgg
      721 ggaggctgga gcgggaactg cggcagggca gcgtgagtga agaaagccag cagtggcttg
      781 cgcagtgcgg gctgacggcg gaacagatgg ccgcgcaact ggaggcggaa tacatcccgg
      841 agaggaaact tcatctttac cactgtgacc accgtgggct gccgctggcg cttatcagcc
      901 cggaagggga aacggcgtgg cagggggagt atgacgagtg gggaaacctg ctgggcgagg
      961 aaagcgcgca gcaccttcag cagtcactcc gtctgccggg acagcagtat gatgaggagt
     1021 cggggctgta ctacaaccgc aaccggtact atgatccgtt gcaggggaga tatatcaccc
     1081 aggacccgat agggctgaga ggtgaatgga atctgtataa atatccatta aaccctgttc
     1141 ggtttataga ttctttggg
//
LOCUS       DBANUU010000390         1157 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.397, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000390 DBANUU010000000
VERSION     DBANUU010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.397"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..1032
                     /gene="gltS"
                     /locus_tag="ACIE68_005029"
     CDS             <1..1032
                     /gene="gltS"
                     /locus_tag="ACIE68_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFX5911162.1"
                     /translation="FTFDASLQSLLMLTFFSSIGLSSDFSRLIKGGKPLVLLTIAVTI
                     LIAIQNTVGMSMAVMMNESPFIGLIAGSITLTGGHGNAGAWGPILADKYGVTGAVELA
                     MACATLGLVLGGLVGGPVARHLLKKVSIPKTTEQERDTIVEAFEQPSVKRKINANNVI
                     ETISMLIICIVVGGYISALFKDTFLQLPTFVWCLFVGIIIRNTLTHVFKHEVFEPTVD
                     VLGSVALSLFLAMALMSLKFGQLASMAGPVLIIIAVQTVVMVLFACFVTFKMMGKDYD
                     AVVISAGHCGFGMGATPTAIANMQTVTKAFGPSHKAFLVVPMVGAFIVDISNSILIKI
                     FIEIGTYFT"
BASE COUNT          303 a          225 c          238 g          391 t
ORIGIN      
        1 tttacttttg atgcttcatt gcaaagttta ttaatgctca cattcttttc ctctatcggg
       61 ctaagttctg acttttctcg actgattaaa ggaggaaagc cgttagttct attaactatt
      121 gcagtaacga tcctaatcgc cattcaaaat actgtcggca tgagtatggc tgtcatgatg
      181 aatgaaagtc catttattgg cttaattgca ggttcaatta ctctaacagg tggtcatggt
      241 aatgccggag catggggccc tattctcgct gataaatatg gtgtaacagg cgccgttgaa
      301 ttagcgatgg cttgtgcaac acttggatta gtgttgggtg gtttagttgg cggccctgtt
      361 gcccgtcatc ttttgaaaaa ggtctctatt cctaaaacaa ccgagcaaga gcgcgacact
      421 atcgttgaag cttttgagca accaagcgtc aaaagaaaaa tcaatgcaaa taacgttatt
      481 gaaaccattt caatgctgat tatctgtatt gttgttggtg gctatatcag tgcattgttt
      541 aaagatactt ttctgcaact gcctactttt gtctggtgtt tatttgtcgg tattattatc
      601 cgtaatacac tgactcatgt atttaaacac gaagtgtttg agccgaccgt cgatgtatta
      661 ggtagcgttg ctttatcgct tttcttggca atggcgttaa tgtcattaaa atttggtcaa
      721 ttggcaagca tggcagggcc agtattaatt atcattgctg tacaaactgt tgtcatggtg
      781 ctatttgcct gctttgtcac cttcaaaatg atgggcaaag attatgatgc tgtcgtgatc
      841 agcgcgggtc actgtggctt tggtatggga gcaacaccaa cagcaattgc gaatatgcaa
      901 acagtcacaa aagcatttgg accatcacat aaagctttcc ttgtcgttcc tatggtcggt
      961 gcctttattg ttgatatttc aaacagtatc ttaattaaaa tatttattga aattggtaca
     1021 tactttacct aatagctaca cactactcgg cgtacttctc ctaacaattt attatagggc
     1081 agtatgccga gccgctttct taccccgtat attcccctag tcaatctatt tagcttgcac
     1141 gtttacgaat taattct
//
LOCUS       DBANUU010000391         1136 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000391 DBANUU010000000
VERSION     DBANUU010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.398"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            165..524
                     /locus_tag="ACIE68_005030"
     CDS             165..524
                     /locus_tag="ACIE68_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911163.1"
                     /translation="MRMTSRKKEILSYFEPDNLEWVTGEIGAPPFDVSGVAYLLHGMV
                     SFDKRHQLESTRRTLESMVAGGLLERVTVYESRQIRRGGETNATVVRYGLPGHCAVMR
                     DTGGADNAISGEYMRVS"
     gene            complement(517..1059)
                     /locus_tag="ACIE68_005031"
     CDS             complement(517..1059)
                     /locus_tag="ACIE68_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HFX5911164.1"
                     /translation="MYQLKITIRDSKPPIWRRVLVPEQIPFSKLHAVIQLAFGWNDEH
                     LYMFEKGRKGDPGSEYRVWGEDESMGNAAITPLWAALQNEGDKLVYTYDFGDWWDCVI
                     VLEKQTHDTSNQPISCLRGKGTTPAENSGGLHGYNELLLQARESDNPEQAEIHNFLML
                     DIERRVYDLSRINDRLQAIN"
BASE COUNT          272 a          289 c          275 g          300 t
ORIGIN      
        1 ttttttattg ttcatttttt atagataccc ccaaaagtac ccccatatct ttttccgccc
       61 ctgaaatttg gttgttattc atacagcata tcaggcggta acgctctccc tttaacccac
      121 ttattgtatg atagtgctat cccacaataa ggaatcagcc agccatgaga atgaccagcc
      181 gcaaaaaaga gatacttagc tactttgaac cggataacct cgaatgggtg actggtgaga
      241 ttggtgcgcc gccgttcgat gtgtcgggtg tggcctacct gcttcatggc atggtgtcgt
      301 tcgataagcg tcaccagctc gaatcaaccc ggcgcacact ggaaagcatg gttgctggtg
      361 gactgcttga gcgggtgaca gtgtatgaat cgcgccagat acggcgcggc ggtgagacta
      421 acgcaacggt cgtgcgttac ggtttacccg gtcattgtgc tgttatgcgc gatactggcg
      481 gcgctgataa cgcaatatca ggtgaataca tgcgggtcag ttaatagcct gcaatctgtc
      541 gttgatgcgg ctcaggtcgt aaacccggcg ttcaatatcc agcatcagga aattgtggat
      601 ctcggcctgc tcaggattat cagactctct ggcctgtaat agcaattcat tgtaaccatg
      661 caagccgcct gagttttccg ccggggtggt gcctttccca cgcaggcagc taatgggctg
      721 attactcgta tcgtgtgttt gcttctccag cacaatgacg caatcccacc agtcgccaaa
      781 gtcatacgtg taaaccagct tgtcaccctc attctggagc gccgcccata gtggcgtgat
      841 tgccgcgtta cccatgcttt catcctcacc ccatacgcga tactcgctac ctggatcacc
      901 tttacgccct ttctcgaaca tatacaggtg ttcgtcattc cagccaaaag ctaactggat
      961 cacggcatga agtttactaa aggggatttg ctctggaacc agcacacgcc gccatatagg
     1021 tggtttgctg tctcggatgg ttatctttag ctggtacatg caaatcctga ataaatgcga
     1081 atcggaagga taacgatact gacgtactca caattctgta aaggtgaata aaggaa
//
LOCUS       DBANUU010000392         1121 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.399, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000392 DBANUU010000000
VERSION     DBANUU010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.399"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            503..787
                     /locus_tag="ACIE68_005032"
     CDS             503..787
                     /locus_tag="ACIE68_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HFX5911165.1"
                     /translation="MQMKNNTAQATKVITAHVPLLMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNLLPVPR"
     gene            787..1062
                     /locus_tag="ACIE68_005033"
     CDS             787..1062
                     /locus_tag="ACIE68_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFX5911166.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
BASE COUNT          285 a          264 c          300 g          272 t
ORIGIN      
        1 tggacgtact gaatacacgc tcgtaagcgg ccctcacggc ccgctaacgc ggagatacgc
       61 cccgacttcg ggtaaaccct cgtcgggacc actccgaccg cgcacagaag ctctttcatg
      121 gctgaacgcg ggtatcgtct agcagggatg gggatgggta aggtgaaatc tatcaatcag
      181 taccggctta cgccgggctt cggcggtttt actccggtat catatgcaac aactgagtgc
      241 cgccttccat gccgctaacg cggcatctgt tggtggtaat atccgaaaag ttatgcatgt
      301 gtatataggg tgtcagcgcc agtgatataa gacggtaatg gggaggctgg tgattttagg
      361 acgactgtag cagcttacat ctggcttttc agtgccatat aaaacagcaa cgtgctgcct
      421 tacatgtgct gacgcgcatt atttgccttg tttcgtctga aagtaatcac tatgattaaa
      481 tatgattaac aactaatcgg atatgcaaat gaaaaacaat accgcacaag caacaaaagt
      541 aattaccgcg catgtgccat tacttatggc tgataaagtc gaccagatgg ccgccagact
      601 ggaacgctcc cggggctggg ttatcaaaca ggcgctttct gcatggcttg cccaggagga
      661 ggagcgtaat cgcctgacgc tggaagccct ggacgatgtg acatccggac aggttatcga
      721 ccatcaggct gtacaggcct gggcggacag cctcagtact gacaatctgt taccggtgcc
      781 acgctgatgg aactgaagtg gaccagtaag gcgctttctg atttggcgcg gttatatgat
      841 tttctggtgc tggccagtaa acctgcggcc gccagaacgg tacagtccct gacacaggca
      901 ccggtcattc tgttaactca tccacgtatg ggagaacagt tgtttcagtt tgaacccagg
      961 gaggtcagac ggatttttgc tggcgagtac gaaatccgtt acgaacttaa tggccagact
     1021 atttatgtat tgcgcctgtg gcacacacga gaaaacaggt agattgtgtg tggggacaac
     1081 ttaacatagc ttgcagatat taattctgga aaattattat c
//
LOCUS       DBANUU010000393         1094 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000393 DBANUU010000000
VERSION     DBANUU010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.400"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            320..607
                     /locus_tag="ACIE68_005034"
     CDS             320..607
                     /locus_tag="ACIE68_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911167.1"
                     /translation="MINEAKKIAVEMMFWTACPVDDDNCFDAIINASKIDREFEDKHA
                     IYLHPIYRDLLSTDQIKSALKKRAIKIISFKCDINEDSARKILMQTILRAV"
BASE COUNT          326 a          210 c          259 g          299 t
ORIGIN      
        1 ataaggggaa aataaattat gaaaggtgtg ttcaggttgc tggaacaacc tgaacacgtt
       61 atcgacaatc aaaactgaat gaggttttta aatgtcaaca cgacagatcg tacagcttgc
      121 tgattctctt gtcaaaggct gggaaaccag aatcccggca atgacgttta gtgagtggga
      181 tcaattccgg tggtggttaa actacctgca agggtatgaa atgttttaaa taaaaagggg
      241 catttctgcc ccttcatata tttagacaaa aatgctatgt gtaactatag tcgtctgcaa
      301 gtaataaagg gggcagggga tgattaatga agctaaaaaa attgctgtgg aaatgatgtt
      361 ttggacagct tgtcctgtgg atgatgataa ttgttttgat gctattatta atgcatcaaa
      421 aattgacaga gaatttgaag ataaacatgc gatttatctt catccgattt acagggattt
      481 attatcaaca gatcaaataa aaagtgctct gaaaaagaga gcaataaaaa taatttcctt
      541 caagtgtgat attaatgagg attcggcaag aaaaatttta atgcaaacca tattaagagc
      601 ggtttaactt ctggtgtgtt gcacccgtag ctcgctggcg ctcacacgcg cagtacagcg
      661 ccccgtccag ggaaaccctg gacggcgtaa actcgctggc gcgagcgcta actgcggcat
      721 tctgcggcgc aaatcagcgt catcgttgtc tgtgagtcag tcgggcttag caggggtgga
      781 aggtggccca ggttccggtt gataacagta gcggcgtggc cgcgcttcgg ggcgatgtga
      841 ctcctgtgct cccctggtat tttcgttcgc cccttacatc cgctggcgcg gataaaaagc
      901 gtgacaatca tcaaataatt taccgatttt caggccagaa accttgctaa tacatctaaa
      961 tagatgtatt attatttcat cgaaagggga ttggctcctt tccttcttaa gtccaaacgg
     1021 acaggagatt taaaatgaaa ttcacttcaa ccacgaatca tgttttcaca ttcgaacgag
     1081 taacgctttg cacg
//
LOCUS       DBANUU010000394         1093 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.401, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000394 DBANUU010000000
VERSION     DBANUU010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.401"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..256
                     /locus_tag="ACIE68_005035"
     CDS             <1..256
                     /locus_tag="ACIE68_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911168.1"
                     /translation="QTDRGLKMSSEVIKQIQKIQDRGIIIYSKFRAAEFDQDDVYRES
                     YFLVVEFNELIAENIIHDEKMVDQTACILHELRRIAIEGK"
     gene            332..634
                     /locus_tag="ACIE68_005036"
     CDS             332..634
                     /locus_tag="ACIE68_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcgAII protein"
                     /protein_id="HFX5911169.1"
                     /translation="MDTFDSEKFLSELDVLSKEIMPGCGLVFELYQRRLSAAIDKVVA
                     GLPEEQHAQAFELARQEFDYLSAEEIAEEIRRDSERGYCCHGFDRDCCPLGCGDLP"
     gene            906..1055
                     /locus_tag="ACIE68_005037"
     CDS             906..1055
                     /locus_tag="ACIE68_005037"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911170.1"
                     /translation="MLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQS
                     RTDLI"
BASE COUNT          300 a          212 c          255 g          326 t
ORIGIN      
        1 ccaaacggac aggggattaa aaatgagttc agaagtaatt aagcaaattc agaaaattca
       61 agatcgcggc attatcatct attcaaagtt tagagcggca gaatttgatc aggatgacgt
      121 ttacagagag tcatattttc tggtcgtaga gtttaacgag ttaatagcag aaaacatcat
      181 tcatgacgaa aaaatggtag atcaaacggc ttgtattctt catgagctta gaagaatcgc
      241 tatagaaggg aaataaatat aacaggggct tatggcccct gttattaact gatgcagaaa
      301 tagtgtctgg taacttatga aggtgaagat gatggatact ttcgatagtg aaaaatttct
      361 ctcagagctg gatgttttat caaaagagat catgcctggt tgtggtcttg tttttgagct
      421 ataccaacgt cgtttgagtg ctgcgattga taaagttgtc gccggattgc cggaagaaca
      481 acacgcccag gcgtttgaac tggctcgcca ggaatttgat tatctttctg cggaagagat
      541 tgctgaagaa atacgccgtg actcagaacg tggttattgc tgtcacggct ttgatcgcga
      601 ttgctgccct ctcggttgcg gtgatttacc atgaaatatg ggaaggggct tgtagcccct
      661 tctcacaagc gctattcaga tctggccgtc ttcctcttcc tcgcccacta ccgttcggtc
      721 gctggggcta gcgattgata atattgtgta ataatacacg tcgttattgg ttaagttttt
      781 aatgtgtggt taatgctaat gtatgcatac attagcatca agtttgttgt gctgaacgta
      841 caataattct ctatatccaa actgacccct atcagagcaa ccaggattca ggtgcgcttt
      901 ttaagatgct ccaccaggtc aataatgagc tgcatgactt actggatagc ctggagaatc
      961 atacccctct tgtcagaaaa aacgctgatg agctgatcgc gctgttactg cttttcaccc
     1021 ggcaggttgg tcagtcccga actgacctga tttgatggtc ctttccctcg gaattgttat
     1081 cttattgtta aca
//
LOCUS       DBANUU010000395         1071 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.402, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000395 DBANUU010000000
VERSION     DBANUU010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.402"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            65..490
                     /locus_tag="ACIE68_005038"
     CDS             65..490
                     /locus_tag="ACIE68_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFX5911171.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            487..837
                     /gene="tnpB"
                     /locus_tag="ACIE68_005039"
     CDS             487..837
                     /gene="tnpB"
                     /locus_tag="ACIE68_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFX5911172.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            868..>1071
                     /locus_tag="ACIE68_005040"
     CDS             868..>1071
                     /locus_tag="ACIE68_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFX5911173.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     TRAEEKIEEDEREIEHLRAQIEKL"
BASE COUNT          292 a          261 c          272 g          246 t
ORIGIN      
        1 tatgtagcca tcagacgaga attggtaact tagacgccca tctgatatag acggacatct
       61 aagtatggaa ttacaggatt ggcgaaaaga acctcgtaaa aactattcga atgaattcaa
      121 acttcgtatg gtggaactgg catcacaacc tggagcttgt gttgcacaga ttgcacgtga
      181 aaatggcgtc aatgataatg ttattttcaa atggctcagg ctctggcaga acgaagggcg
      241 tgtttcgcgg cgtcttccgg taacgacctc ttctgacact ggcgttgaat tattacctgt
      301 agaaataacg ccggatgagc agaaagaacc tgtggcggcc attgcgccgt ctttatccac
      361 ttccactcag accagagtca gtgccagttc ctgcaaggtg gaattccgtc acggtaacat
      421 gacgctggaa aatccatcgc cagagctgct cacagtgttg atccgtgaac tgaccgggag
      481 gggaagatga tctcactccc atcaggtacc cgtatctggc tcgttgccgg cgttaccgat
      541 atgcgtaaat ccttcaacgg actgggagaa caggtacaac atgtgctgaa tgataatccc
      601 ttctccggtc acctgtttat cttccgtggc cgacggggtg acaccgtcaa aattctttgg
      661 gctgatgctg atggtctgtg cctgttcacc aaacgcctgg aggaaggcca gtttatctgg
      721 cctgcggtac gtgacggcaa ggtatccatt acccgctcgc aactggcaat gctcctcgat
      781 aagctggact ggcgtcagcc aaaaacatcc agccgtaact cactgacaat gttgtaaaaa
      841 actcctgacc gcattataaa aacggtcatg agtcagaaat acctcattcg catcgcagag
      901 ctggaaaggt tgctctctga gcaggctgaa gccctccgtc agaaagacca gcaactgagt
      961 ctggttgaag agacggaagc cttcctgcgc tctgcactga cacgtgccga agaaaagatc
     1021 gaagaagatg aacgggaaat agaacatctg cgggctcaga tagaaaaact g
//
LOCUS       DBANUU010000396         1066 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000396 DBANUU010000000
VERSION     DBANUU010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.403"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..299)
                     /locus_tag="ACIE68_005041"
     CDS             complement(<1..299)
                     /locus_tag="ACIE68_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFX5911174.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRDGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQEL"
     gene            688..921
                     /locus_tag="ACIE68_005042"
     CDS             688..921
                     /locus_tag="ACIE68_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFX5911175.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLCSEKRVSSEANKEKSDITELLRKQVRPD"
     gene            979..>1066
                     /locus_tag="ACIE68_005043"
     CDS             979..>1066
                     /locus_tag="ACIE68_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFX5911176.1"
                     /translation="MDQVVIFKQIFDKVRNDLNYQWFYSELKR"
BASE COUNT          326 a          265 c          215 g          260 t
ORIGIN      
        1 ttcagttcct gtgcgtcggc ctgcgcacgc gtaagtcgat ggcgttcgta ctcaatagtc
       61 cctggctgga gatctgcctc gctggcctgc cgcagttctt caacctcccg gcgcagcttt
      121 tcgttctcaa tttcagcatc cctttcggca taccatttta taacggcggc agagtcataa
      181 agcacctcat tacccttgcc accgtctcgc agaacgggca ttccctgttc ctgccagttc
      241 tgaatggtac ggatactcgc accgaaaatg tcagccagct gctttttgtt gacttccatt
      301 gttcattcca cggccaaaaa cagagaaagg aaacgacaga ggccaaaaag cccgttttca
      361 gcacctgtcg tttcctttct tttcaggggg tattttaaat aaaaacatta agttacgacg
      421 aagaagaacg gaaacgcctt aaaccggaaa attttcataa atagcgaaaa cccgcgaggt
      481 cgccgccccg taacctgtcg gatcaccgga aaggacccgt aaagtgataa tgattatcat
      541 ctacatatca caacgtgcgt ggaggccatc aaaccacgtc aaataatcaa ttatgacgca
      601 ggtatcgtat taattgatct gcatcaactt aacgtaaaaa caacttcaga caatacaaat
      661 cagcgacact gaatacgggg caacctcatg tcaacgaaga acagaacccg cagaacaaca
      721 acccgcaaca tccgctttcc taaccaaatg attgaacaaa ttaacatcgc tcttgagcaa
      781 aaagggtccg ggaatttctc agcctgggtc attgaagcct gccgccggag actgtgctca
      841 gaaaaaagag tttcttctga agcaaacaaa gaaaagagtg acattactga attgctcaga
      901 aaacaggtca gaccagattg aagcaattta gataatcgtg cagactacgc cccctcatat
      961 cacatggaag gtttatctat ggatcaggta gtcattttta aacaaatatt tgataaagtt
     1021 cgaaacgatt taaactatca atggttttat tctgagctaa aacgtc
//
LOCUS       DBANUU010000397         1032 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000397 DBANUU010000000
VERSION     DBANUU010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.404"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..205
                     /locus_tag="ACIE68_005044"
     CDS             <1..205
                     /locus_tag="ACIE68_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911177.1"
                     /translation="NLARVIDQNALEIIFAAGIQQRAATNMLIKPLVVSIIRQRPVME
                     YDASHLGNMVNRLEEALPPELPA"
     gene            281..736
                     /locus_tag="ACIE68_005045"
     CDS             281..736
                     /locus_tag="ACIE68_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFX5911178.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            815..>1032
                     /locus_tag="ACIE68_005046"
     CDS             815..>1032
                     /locus_tag="ACIE68_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFX5911179.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDG"
BASE COUNT          271 a          264 c          250 g          247 t
ORIGIN      
        1 gaatctggcc cgtgttatcg atcagaatgc tctggagatc attttcgccg ccggtataca
       61 acaacgggcc gcgaccaata tgttaatcaa accactggtg gtcagtatca tcaggcaacg
      121 ccccgtcatg gaatatgatg catcccatct cggcaatatg gttaaccgac tggaagaagc
      181 attacccccg gagttaccgg cataacggct attctgcgcc ttttacgctt tatcacctga
      241 tttgtcagtg tcatcgtcat gactcagaga ggtaaatacc atgattcacc tgttcaaaac
      301 ctgcatgatt accgccttca ttctggggtt aacgtggtct gccccactcc gggcacagga
      361 tcaacgttac atcagtatac gcaatacaga tacgatatgg ctcccgggaa atatttgtgc
      421 ttaccagttc cggctggata atggcggaaa cgatgaagga tttggccccc tcaccatcac
      481 tctgcaactc aaagacaaat atggtcagac gctggtgacc agaaaaatgg aaacggaagc
      541 ttttggtgac agtaatgcca cgcgaaccac agacgcattt ctggaaacgg agtgcgtgga
      601 aaatgtcgcc acaaccgaaa tcattaaagc aactgaagaa agtaacggcc atcgtgtcag
      661 tctgccgtta tcggttttca atccccagga ctaccatcca ctgctgatta ccgtttccgg
      721 aaaaaacgtt aactgatccc tgaagtcagt ttcatgcttc agcctctgtc acaacgcgtg
      781 gcagaggctt tttatttatc aggagaaaac agatatgccg ggttacacag agtatgtcct
      841 ggctgaagga tctttctcat acgggcaggc ggttgctgtc ataaccgctt accgcaatgt
      901 gtttatccag gatgacccgg gaatgcattt ccgtcgggtt atccgcaacg cggaaggtca
      961 gcgacgctgg cgctgccgga attctgaacc cgatgccgga aaggtgctta atacgcggct
     1021 cgccagtgac gg
//
LOCUS       DBANUU010000398         1027 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000398 DBANUU010000000
VERSION     DBANUU010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.405"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(471..>1027)
                     /gene="hdeD"
                     /locus_tag="ACIE68_005047"
     CDS             complement(471..>1027)
                     /gene="hdeD"
                     /locus_tag="ACIE68_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFX5911180.1"
                     /translation="ATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFVSGDILS
                     TVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPELGIFAIA
                     AFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVSVTLVST
                     LVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT          336 a          234 c          211 g          246 t
ORIGIN      
        1 tcgagtctcc ttgtttcgac ttaagctggc aattggattg ccagctttct tttaacgcat
       61 tgaaattacc tgagtaaata acgcattcat gtcttcaggt ttttatatag cgtctggaga
      121 cacggatatt tatgcaatgt aatcaatgcc agttgcaaaa aaatgaaccc ggattactta
      181 tttaaccgcc catagcatca acgcttacgg aactctttgt tcactttccc actgcacttt
      241 taaaacatta aacctgacta cggaaaatat cagccatgat aatgtttgaa atggctaatt
      301 tgccatggag tgaaaaaaat tagatgaaat tcagtaggtt gaaataatca ctagcaggta
      361 attatttcaa tgatagtgcg caattgatct acaacgctgc gtagcggtga gagtattaat
      421 cggatcatag tcacatcaag tgactatgat ccgggtgaca accgggataa ttattgctgc
      481 ttaacgaaca aactggcgaa gctgaacagg ctggcggcgc taaatatcag ttcaattccc
      541 accagtgtgg aaaccagcgt tacagacacc atcggcgttg caccaaggaa tatccaggca
      601 atgacgatat ccagcacacc aataacgagc tgtagccagc tgcctttcat tgaacgctga
      661 cgataccaac tcatcaggcg aataacccct gcaacacaga acaaaccggc aataaatgcc
      721 gcaatggcaa aaatgcccag ctccggtgcg cggatgaaga aatagccgat caataaatag
      781 gcgactgcga cgaggaaacc ggataatacc ggccagaaat tatgactgcg gttgctgaat
      841 aacccgacaa taagcgcaat gcccgagcag attaataatg cacccactac tgtgcttaaa
      901 atatcgccag agacgaacgg gaaactgata cacagcaacc cgacgataaa caacagcacg
      961 gcaataaact ggattgctct gcgatgtttt ttaagcatct ccagatcaaa cttcaaaatt
     1021 gttgcct
//
LOCUS       DBANUU010000399         1023 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000399 DBANUU010000000
VERSION     DBANUU010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.406"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..344)
                     /locus_tag="ACIE68_005048"
     CDS             complement(<1..344)
                     /locus_tag="ACIE68_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HFX5911181.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKT"
     gene            complement(341..973)
                     /gene="traW"
                     /locus_tag="ACIE68_005049"
     CDS             complement(341..973)
                     /gene="traW"
                     /locus_tag="ACIE68_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFX5911182.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
BASE COUNT          217 a          333 c          275 g          198 t
ORIGIN      
        1 gttttaccaa aagccggcag gctgaagccc aggttaacca tgcatcccgg tgatcgggtg
       61 acgtccgtca gcgccatcgg ctcccagtaa ccaatggcca gcccgatacg cctgaacagc
      121 ggcggcggtg ccggacaaat ctgaatgggc atcgacgggt tcgccgtgtc ggggacctta
      181 ccctgactga ctttgatact gcccagcgag agcgggaaaa tacagctcca gcagatatct
      241 gtgatcgggt ttacaaaacg cccctcacag gcagaatccg cagacgctgc agggacatga
      301 ccggcgagaa ggaataacat cagcagccac aggcttcgct tcattttctg ccctcctctg
      361 ccggaataaa ttccaccttc aggaagcgat cgccgggaac ggcgctcacc cgtgccggga
      421 cctgatcaat gccgaggcgc tggcagagca caccgttctg gtcaaagtaa acacggctgt
      481 ccagagactt ctgcatctcc gggatactgc cctgcacgag gataattttg ctctccagtg
      541 tgggcggcgt ctggcgtttc atccaggcca cctgcgccgg atcatcgcca ttgatgaaat
      601 acagcgtctg gttaaaagga acatactgca gcggattcat cacttcaccc tgacgggcaa
      661 acacccgccc ttcgttatcc cggatatccg cagccagtct gacggacggg tcaaacaacc
      721 ggctgccgta tttctccgtg cgtccgatac cgggcacggc agggggccgc agactgttgc
      781 ggatcacccg ctccttaaag gcatccattt tccggcccat ctcaccggac tgctccagtg
      841 ccgtcaggcg ctgcatgatc accgtcagca tgtccggctc ctttaccggc cacagatcgc
      901 cccaggtacc aagatcggcg gcggccacac tctgccccca tatcagcagg gcaatcagcc
      961 cccggcatct catggctccg ccctcattcg ccgggcgata tcctgctgga tttcccgggt
     1021 gat
//
LOCUS       DBANUU010000400         1022 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.407, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000400 DBANUU010000000
VERSION     DBANUU010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.407"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            97..1008
                     /gene="hlyE"
                     /locus_tag="ACIE68_005050"
     CDS             97..1008
                     /gene="hlyE"
                     /locus_tag="ACIE68_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFX5911183.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKRHGKKTLFEVPEV"
BASE COUNT          345 a          190 c          221 g          266 t
ORIGIN      
        1 cgcccggcta actatgaatt agatgaagta aaatttatta atagttgtaa aacaggagtt
       61 tcattacaat ttatatattt aaagaggcga atgattatga ctgaaatcgt tgcagataaa
      121 acggtagaag tagttaaaaa cgcaatcgaa accgcagatg gagcattaga tctttataat
      181 aaatatctcg atcaggtcat cccctggcag acctttgatg aaaccataaa agagttaagt
      241 cgctttaaac aggagtattc acaggcagcc tccgttttag tcggcgatat taaaacctta
      301 cttatggata gccaggataa gtattttgaa gcaacccaaa cagtgtatga atggtgtggt
      361 gttgcgacgc aattgctcgc agcgtatatt ttgctatttg atgagtacaa tgagaagaaa
      421 gcatccgccc agaaagacat tctcattaag gtactggatg acggcatcac gaagctgaat
      481 gaagcgcaaa aatccctgct ggtaagctca caaagtttca acaacgcttc cgggaaactg
      541 ctggcgttag atagccagtt aaccaatgat ttttcagaaa aaagcagcta tttccagtca
      601 caggtagata aaatcaggaa ggaagcgtat gccggtgccg cagccggtgt cgtcgccggt
      661 ccatttggtt taatcatttc ctattctatt gctgcgggcg tagttgaagg gaaactgatt
      721 ccagaattga agaacaagtt aaaatctgtg cagagtttct ttaccaccct gtctaacacg
      781 gttaaacaag cgaataaaga tatcgatgcc gccaaattga aattaaccac cgaaatagcc
      841 gccatcgggg agataaaaac ggaaactgaa acaaccagat tctatgttga ttatgatgat
      901 ttaatgcttt ctttgctaaa agaagcggcc aaaaaaatga ttaacacctg taatgagtat
      961 cagaaaagac acggtaagaa gacactcttt gaggtacctg aagtctgatc agcgattatt
     1021 ct
//
LOCUS       DBANUU010000401         1009 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.408, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000401 DBANUU010000000
VERSION     DBANUU010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.408"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>1009
                     /locus_tag="ACIE68_005051"
     CDS             <1..>1009
                     /locus_tag="ACIE68_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFX5911184.1"
                     /translation="AESWEDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPA
                     KFHECFINWMRDCHSSDDKDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVIGQ
                     IKTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQG
                     RLNKAFEEKFPLKELNNPAHDCSAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLK
                     KLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVV
                     MNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAG
                     SG"
BASE COUNT          331 a          178 c          245 g          255 t
ORIGIN      
        1 gtgcagaaag ttgggaagat atagaggatt tcggggaaac acatctcgat ttcttgaagc
       61 aatatggtga ttttgaaaat ggtattcctg ttcacgatac tattgccaga gttgtatcct
      121 gtatcagtcc tgcaaaattt catgagtgct ttattaactg gatgcgtgac tgccattctt
      181 cagacgataa agacgtcatc gcaattgatg gaaaaacgct ccggcactct tatgacaaga
      241 gtcgccgcaa gggagcgatt catgtcatta gtgcgttctc aacaatgcac agtctggtca
      301 tcggacagat caagacggat gagaaatcta atgagattac agctatccct gaacttctta
      361 acatgttaga tattaaagga aaaattatca caactgatgc gatgggttgc cagaaagata
      421 ttgcagagaa gatacaaaaa cagggaggtg attatttatt cgctgtaaaa ggaaaccagg
      481 ggcggcttaa taaagccttt gaggaaaaat ttccgctgaa agaattaaat aatccagcgc
      541 atgactgttc cgcaattagt gaaaagagtc acggcagaga agaaatccgt cttcatattg
      601 tttgcgatgt ccctgatgaa cttattgatt tcacgtttga atggaaagga ctgaagaaat
      661 tatgcgtggc agtctccttt cggtccataa tagcagaaca aaagaaagag ccagaaatga
      721 cggtcagata ttatatcagt tctgctgatt taactgcaga aaagttcgcc acagcaatcc
      781 gaaaccactg gcacgtggag aataagctgc actggcgtct ggacgtggta atgaatgaag
      841 acgactgcaa aataagaaga ggaaatgcag cagaattatt ttcagggata cggcacattg
      901 ctattaatat tttgacgaat gataaggtat tcaaggcagg gttaagacgt aagatgcgaa
      961 aagcagccat ggacagaaac tatctggcgt cagtccttgc ggggagcgg
//
LOCUS       DBANUU010000402         1000 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.409, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000402 DBANUU010000000
VERSION     DBANUU010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.409"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..410)
                     /locus_tag="ACIE68_005052"
     CDS             complement(<1..410)
                     /locus_tag="ACIE68_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFX5911185.1"
                     /translation="MMMPVLQKLPFSGLPFSGICGYSFSAAAKSAAGRGNPSMLSATY
                     DAPCVFFCVAINATERQIMVWCVVSRANGTPCILPASAHYTAESMVVQAGQPPGWPVS
                     DNAGISTPVWAIAIERGNSGDSVIYAVIGGCLMAT"
     gene            complement(407..673)
                     /locus_tag="ACIE68_005053"
     CDS             complement(407..673)
                     /locus_tag="ACIE68_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFX5911186.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
BASE COUNT          270 a          251 c          265 g          214 t
ORIGIN      
        1 gtagccataa ggcaacctcc gataacagcg taaatgacgc tatcgccgga gtttccacgc
       61 tcgatggcga tagcccagac gggggtggaa ataccggcgt tatcggaaac cggccagccc
      121 ggaggctgcc ccgcctgaac taccattgac tctgcggtat aatgagcgga cgcgggcaga
      181 atgcacggag tgccatttgc tcgactgacc acacaccaca ccataatctg gcgctctgtg
      241 gcgttgattg cgacacaaaa aaagacgcat ggcgcgtcat atgtcgccga taacatactc
      301 gggtttccac gcccggctgc cgattttgcg gcagcggaaa aactatatcc gcaaatgccg
      361 gaaaaaggca agccagaaaa aggaagtttt tgcagaacag gcatcatcat gcgtcgtacc
      421 cccgtttgcg tccggcaatg cgcccggcca tccatgcggt gacttcagag tgcagccagg
      481 ccacattttt accgccaaga ctcacctgcg gcggaaattc ccccttacgg atgagttcgt
      541 agatggtcga gcgtgacagg ccgcacaggt gcatcacttc cggcagacgt aaaaaacgct
      601 cctgcgtgat gtccggcagc ggcatcagtg gcgtcacagg ggcgggagac ggggaagaaa
      661 aaacagcttg catcgggcta cctcgttaat gtccatacag caccggataa gtccgtccgg
      721 cttcgggtag cgctttattt tgtgaatatt ttcggcagac gcaacagggg ggatttgttc
      781 cggcagcctt acaatggctg tgtatttttt gtacatcagc gccagatagc tttaaaacgc
      841 tctggaagga gctggaaaaa attatagtga aatacaaatt gttttttctt atttatttca
      901 gtgaattaat aaaaataaaa agtaataaac agcacaaaaa gcccatcaac gggtgaacag
      961 tggtgaacag acggtgaaca gtcattactg cgattgttca
//
LOCUS       DBANUU010000403          999 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.410, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000403 DBANUU010000000
VERSION     DBANUU010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.410"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            56..>999
                     /gene="bluF"
                     /locus_tag="ACIE68_005054"
     CDS             56..>999
                     /gene="bluF"
                     /locus_tag="ACIE68_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFX5911187.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQCDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDFYTLSPNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYKAD
                     LKSKALAFAMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGL"
BASE COUNT          277 a          215 c          213 g          294 t
ORIGIN      
        1 gaagatatgt ctgttaccgt cttactctca cctctcccat taccctggac agttaatgct
       61 taccaccctt atttatcgta gccatatacg tgacgacgaa cctgtcaaaa aaatcgaaga
      121 aatggtttcg atagcaaatc gcaggaacat gcagtgtgac gtaacaggga tcttactgtt
      181 taatggttct cattttttcc agcttctgga aggtccggaa gaacaggtta aaatgatata
      241 tcgggctata tgtcaggatc cacggcacta taatattgtt gaactgctgt gcgattacgc
      301 gcctgctcgc cgttttggca aagcgggaat ggaattattt gatttgcgct tgcacgagcg
      361 agatgacgtt ttacaggccg tattcgacaa aggcacatca aaatttcagc taacttatga
      421 tgacagagcg ctacaatttt ttcgtacttt tgtccttgca accgaacaat caacctattt
      481 cgagatccct gccgaagatt cctggctttt tatcgctgac ggatctgata aagaacttga
      541 tttctatacc ctttcaccaa atataaacga ccactttgcc tttcatccaa ttgtcgatcc
      601 cttatcgcgg cggataatcg cttttgaagc cattgtgcaa aaaaatgaag atagcccatc
      661 agctatagcg gttgggcagc gtaaagacgg ggaaatctac aaagcggatc tcaaaagtaa
      721 ggcgcttgca ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa
      781 tctattacct atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttaatgaaat
      841 aaaggccaat gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagtcat
      901 atctcggttt gatgagtttg ccgaagcgat taaatcgcta aaggctgctg gtatcagtgt
      961 agcaattgat cattttggcg caggttttgc tggtttgtt
//
LOCUS       DBANUU010000404          999 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.411, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000404 DBANUU010000000
VERSION     DBANUU010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.411"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..227
                     /locus_tag="ACIE68_005055"
     CDS             <1..227
                     /locus_tag="ACIE68_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911188.1"
                     /translation="MKKNETWMLLNGDATPNIRTMSNKKIECLEKSEVEWASRVCDSI
                     TDKERENLKALICSKPAKSVNTGKRPALGY"
     gene            270..845
                     /locus_tag="ACIE68_005056"
                     /pseudo
     CDS             270..845
                     /locus_tag="ACIE68_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014622.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          332 a          168 c          244 g          255 t
ORIGIN      
        1 ttatgaagaa aaatgaaaca tggatgttat taaatggaga tgcaacacca aacattagaa
       61 caatgagcaa taagaaaatt gaatgcctgg agaagagtga agtagaatgg gctagtaggg
      121 tttgcgacag tatcacagac aaggaaaggg aaaatctgaa ggcattaata tgtagcaaac
      181 cagctaaatc agttaataca ggcaaaagac cagcgttagg atattgaaac cggcactgca
      241 ctatatcact attacaatat tctgagtgac tagagcaact gctccgccat ccgggctagc
      301 agtatgacga cgaatccgaa ctgcactaca actggcagcg gtactacaat ccgggacagg
      361 gacgatatat cacccaggac ccgatagggc tggcgggggg attaaacccg tacagttatg
      421 ttatgaacaa tcctgtgcaa tggattgatc cattgggatt agctcatact tcaggaaaat
      481 ggaaggactg tggtaaaggt tgtcgaatcc gaatagatgg cgatgctgtt ggtgacggaa
      541 gacatttgca ttgggagtgt aaaaatggga gtggtgtaat gggggaattt ggaggtatgt
      601 cccatggtga ggattactca tcagcaccaa atagcattaa gaaatgtgca aaaaaatatg
      661 gtttcgagcc agaaccaagt aaagatctgt catgcaaaac ctccgggcta tcaactactg
      721 atgcggctct tgcaactggt gctacaattg gcgcagggta tgttatatat agagtgattc
      781 gatttatacc ttcacttgca ccacctcttt ggtggtcaat acctgcaaac gttgttactc
      841 catgattaaa acggataatg gagattatat aatgagcgat tttgacattg tgagttcaat
      901 tgactggcat gatggggttt tgttagaagt aaaaacgaca ttcaaagaat ctcattttaa
      961 catgacgtta tttattgaca tttataaaag tgagaatat
//
LOCUS       DBANUU010000405          994 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.412, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000405 DBANUU010000000
VERSION     DBANUU010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.412"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..239)
                     /locus_tag="ACIE68_005057"
     CDS             complement(<1..239)
                     /locus_tag="ACIE68_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFX5911189.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASEADLQPG"
     gene            628..861
                     /gene="ynfO"
                     /locus_tag="ACIE68_005058"
     CDS             628..861
                     /gene="ynfO"
                     /locus_tag="ACIE68_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFX5911190.1"
                     /translation="MSTKNRTRRTTIRNIRFPNQMIEQINIALDQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQIRPD"
     gene            918..>994
                     /locus_tag="ACIE68_005059"
     CDS             918..>994
                     /locus_tag="ACIE68_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911191.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYS"
BASE COUNT          317 a          249 c          189 g          239 t
ORIGIN      
        1 cctggctgga gatctgcctc gctggcctgc cgcagttctt caacctcccg gcgcagcttt
       61 tcgttctcaa tttcagcatc cctttcggca taccatttta tgacggcggc agagtcataa
      121 agcacctcat tacccttgcc accgcctcgc agaacgggca ttccctgttc ctgccagttc
      181 tgaatggtac ggatactcgc accgaaaatg tcagccagct gctttttgtt gacttccatt
      241 gttcattcca cggacaaaaa cagagaaagg aaacgacaga ggccaaaaag ctcgctttca
      301 gcacctgtcg tttcctttct tttcagaggg tattttaaat aaaaacatta agttatgacg
      361 aagaagaacg gaaacgcctt aaaccggaaa tttttcataa atagcgaaaa cccgcgaggt
      421 cgccgccccg taacctgtcg gatcgccgga aaggacccga aaaatgataa taattatcat
      481 ctgcatgtca caacgtgcat ctacgccatc aaaccacgtc aaataatcaa ttatgacgca
      541 ggtatcatat taattgatct gcatcaactt aacgtaaaaa caacttcaga caatacaaat
      601 cagcgacact gaatacggga caacctcatg tcaacgaaga acagaacccg cagaacaaca
      661 atccgcaaca tccgctttcc taaccaaatg attgaacaaa ttaacatcgc tcttgatcaa
      721 aaagggtccg ggaatttctc agcctgggtc attgaagcct gccgccggag actgtgctca
      781 gaaaaaagag tttctcctga agcaaacaaa gaaaagagtg acattactga attgctcaga
      841 aaacagatca gaccagattg aagcaattta gataatcgtg cagactacgc ccctcatatc
      901 acatggaagg tactacaatg gctcaggttg ccatttttaa acaaatattc gataaagtgc
      961 gaaataattt aaactatcac tggttttatt ctga
//
LOCUS       DBANUU010000406          992 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.413, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000406 DBANUU010000000
VERSION     DBANUU010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.413"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            2..>992
                     /locus_tag="ACIE68_005060"
     CDS             2..>992
                     /locus_tag="ACIE68_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFX5911192.1"
                     /translation="MALPLLCTTAQANSFLHYAGEFNFRTGEKQNNVTIAGLSAITFD
                     AKKNIFYAINDSRNNNKEGDASLYTLKIQVSSRGIEQVNFLNQRPLLDAKQQPFVTNT
                     VDAEGLALTHNGKSLLWSSELGAPLRLSTLDGVMEKDFTSLFPARFNISSDKESSNGI
                     RSGNAWEGLTVTPDGKSLFIAVESSLKQDGPIASPINSGTARLLQFSIDADGRPSKQL
                     HEYLYITDPVPQVSKFGINDNGVSEVLALNDHQLLVIERSGRNVSAGFNDWDYSVRVY
                     MVDLTAASDIKNIDSLQDWSNKSTLQPVSKKLLIDFADYTSSADCIEGVTFGPL"
BASE COUNT          287 a          210 c          223 g          272 t
ORIGIN      
        1 tctggcatta ccgctgttat gcacaacagc gcaggcaaac agttttcttc attatgctgg
       61 ggagtttaac ttccggacgg gagaaaagca gaacaatgtc acaatcgcgg ggctatcagc
      121 catcacattt gatgcaaaaa aaaatatttt ctatgctatt aacgatagca gaaacaataa
      181 taaagaagga gatgccagtc tttatacact aaaaatacag gtatcatcca ggggcattga
      241 gcaagtgaat tttctcaacc agcgtccact gcttgatgca aaacagcagc ctttcgtgac
      301 caatacggtg gatgccgaag gtctggccct gacacacaat ggtaaatcat tactctggag
      361 ttcggagtta ggtgctccac tgcgtctgag cactctcgat ggcgtaatgg agaaagattt
      421 tacgtcttta tttcctgctc gctttaatat tagttctgac aaagagagca gcaatggtat
      481 tcgcagcggc aacgcctggg agggcctgac agttacacca gatggaaaat cactttttat
      541 cgccgttgaa agcagcctta aacaggatgg gcctattgca agtccaataa attcaggcac
      601 agcacgctta ctgcaatttt ctatagatgc agacggtcga ccttcaaagc aattgcatga
      661 atatctctat attactgatc ctgttccgca agtgagtaag ttcggtatta atgataatgg
      721 cgtatccgag gtcctggcgc tgaatgatca ccaattgttg gtcatcgaac gttccggcag
      781 aaacgtcagt gctgggttca atgactggga ttactctgtc agagtttata tggttgattt
      841 aactgctgcc agcgatatca agaatattga cagtctgcaa gattggtcca ataaatccac
      901 gttacaaccc gtcagtaaaa aattattaat tgattttgct gattacactt cctctgcgga
      961 ttgtattgaa ggggtaacct tcggtcctct ga
//
LOCUS       DBANUU010000407          986 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.414, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000407 DBANUU010000000
VERSION     DBANUU010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.414"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(179..382)
                     /locus_tag="ACIE68_005061"
     CDS             complement(179..382)
                     /locus_tag="ACIE68_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911193.1"
                     /translation="MVSYDKIRAEYRAKYRAYKLELIDDLIAQRDQLNFTFSDLLNSK
                     RDCKRKREYLRLSALIGKLQNSI"
     gene            complement(431..>986)
                     /locus_tag="ACIE68_005062"
     CDS             complement(431..>986)
                     /locus_tag="ACIE68_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911194.1"
                     /translation="YNEHYKNGTHTKADKANMQAATTKLAYFINNVVNAVEDEKLCSV
                     FYYAIKASKQAPEVFFRDAMTNSYSLEKLVYLVKSIKSGKCVYSIADMSGSRVFALID
                     MINDEIDTFTNGAVFDLMNEAKKACEIKLDAGYTQANQLINLCERLGLVEKVKGAGSA
                     KAGTQQYRFIKNDFYNYLADAFKA"
BASE COUNT          280 a          201 c          184 g          321 t
ORIGIN      
        1 acgtctgctt tttttcgctc ctggtcgctc aataagtcaa ttttgcgcgt tgtttcattt
       61 ataagctcga ctgcgcgccc agcgtaacca gcgatcccag cgatgcgatt aacataatat
      121 tcgttatgga cgttaacgcc agcgataaga acaaacatga ttaagctcct taaaaaaatt
      181 aaatagaatt ttgaagtttt ccgattaatg cgcttaaacg taagtattca cgtttgcgct
      241 tgcaatctcg cttgctgtta agcaaatcag aaaacgtaaa atttaattgg tcgcgttgcg
      301 cgatcaaatc atcgattaat tccagtttat aagcgcgata ctttgcgcga tattccgcgc
      361 ggatcttgtc atagctgacc atttttaaag ctcctttaaa gcgcccatag tgggcgcttg
      421 attccattaa ttacgctttg aaagcatcag ctaaataatt gtagaaatca tttttaatga
      481 agcgatattg ctgagtacca gctttcgcgc tcccagctcc tttgactttt tcaacaagtc
      541 caagacgttc gcaaagattg atcaactggt tggcttgagt atagccagcg tccaatttaa
      601 tttcacacgc ttttttagct tcattcatta aatcgaaaac agcgccattg gtgaacgtgt
      661 cgatctcgtc gttaatcata tcgattaaag cgaatacacg agatcctgac atatcagcga
      721 tggaataaac gcatttacca gatttaattg atttaaccag ataaaccagt ttttccagag
      781 aataactatt agtcattgca tcacggaaaa atacttctgg cgcttgtttg cttgctttaa
      841 tcgcatagta aaaaacagag cataattttt cgtcttctac agcgtttaca acgttgttga
      901 tgaagtaagc aagtttagtg gtcgcagctt gcatatttgc tttgtctgct ttagtgtgcg
      961 taccattttt ataatgttcg ttatat
//
LOCUS       DBANUU010000408          986 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.415, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000408 DBANUU010000000
VERSION     DBANUU010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.415"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(125..355)
                     /gene="ynfN"
                     /locus_tag="ACIE68_005063"
     CDS             complement(125..355)
                     /gene="ynfN"
                     /locus_tag="ACIE68_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HFX5911195.1"
                     /translation="MKKIIVFFNSEPAVVVPAMTGVNTIMREYPNGEKTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAIRLLS"
     gene            complement(360..425)
                     /gene="ynfR"
                     /locus_tag="ACIE68_005064"
     CDS             complement(360..425)
                     /gene="ynfR"
                     /locus_tag="ACIE68_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YnfR"
                     /protein_id="HFX5911196.1"
                     /translation="MKAPSGAFLLGVYSMDTHILR"
     gene            complement(428..616)
                     /gene="ynfQ"
                     /locus_tag="ACIE68_005065"
     CDS             complement(428..616)
                     /gene="ynfQ"
                     /locus_tag="ACIE68_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFX5911197.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            complement(627..839)
                     /gene="cspI"
                     /locus_tag="ACIE68_005066"
     CDS             complement(627..839)
                     /gene="cspI"
                     /locus_tag="ACIE68_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HFX5911198.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT          341 a          192 c          172 g          281 t
ORIGIN      
        1 gattatatgg tgcaaacgtg taaaagataa gatgaaacat cgcaataatc aacatacgat
       61 agtctaaatt ttacacaaac agacaaagag aattttcctg aattatcaat gcaatagcat
      121 caaatcaact caagagcctt attgctgctt ccagaatttc ttctgaagta acatgtcgat
      181 ccgcggctac ataaatgact ttatgatctc cggtcagaga tggaaaccct gcggccatta
      241 cagtaaggtg tgttttttcg ccatttggat attcacgcat gatggtgtta actccagtca
      301 tcgctggcac taccactgct ggttcagagt taaaaaaaac tatgattttt ttcatgatgt
      361 taccgtagta tgtgagtatc catcgaatag acaccaagca aaaaagctcc cgaaggagcc
      421 ttcattttca cttttttaaa tccaacgaca gacggctggc atttaagtat tgtgaaatat
      481 tatcaaatgt aatcatcatt gatttacaaa agatacattt tgccccgaaa ggattcatgt
      541 cagaaacatc aaaagatgat gttctatact gggaaccatg acaacacggg catctaaagt
      601 gaatatggtt tgtaatattg tctacctcaa agcgccacta catgaacagc ggcaggacct
      661 ttaggtccgt tctcaatacc aaattcaact tcctgattct cagttaatgt tttgaaatcg
      721 ttgctctgaa ttgctgagaa atggacaaac acatctttgc tgccatcttt cggcgtgatg
      781 aaaccaaaac ctttttcagg gttaaaccat ttcactaaac cagtcatttt gttagacata
      841 attattacct tttgaagaaa ttagcccttg ggcagaatgg tccgaaaaaa aatatcagag
      901 agaaaaacca acaaggaaat ctcaagaggt acaaataata aaattataac aatgactgct
      961 tcagataaat ttgtaacaaa ccagaa
//
LOCUS       DBANUU010000409          976 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.416, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000409 DBANUU010000000
VERSION     DBANUU010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.416"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..82
                     /locus_tag="ACIE68_005067"
     CDS             <1..82
                     /locus_tag="ACIE68_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911199.1"
                     /translation="ERAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(128..>976)
                     /gene="ygcG"
                     /locus_tag="ACIE68_005068"
     CDS             complement(128..>976)
                     /gene="ygcG"
                     /locus_tag="ACIE68_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFX5911200.1"
                     /translation="TFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKELTHKLESIF
                     NNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSDRTVRIKVG
                     YGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQYPTNPSESE
                     SASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDLFLFSDKIF
                     SIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSDSGGFSGGGGSSGGG
                     GASGRW"
BASE COUNT          298 a          205 c          211 g          262 t
ORIGIN      
        1 ggaaagggcc ttcccgagag tggtaaagga gaggccctgg aaatacccca cagccccgaa
       61 aaagagccag tcagttgctt aactgactgg cattacagcc tccgggctgg cattttttat
      121 ggttgagtta ccagcggccg gatgcgccgc cacctccaga agaaccgccc ccgccgctaa
      181 atccgccgct atctgagcct ccagcggaac cgctgttgtc tgaatgataa cttctaccag
      241 atgctctttt ctgaagtaca cataaacagg taaagaccat aaatatggtg aaagtaaaaa
      301 agaaggaaaa tacagcaatg gaaaaaattt tatcggagaa caggaataaa tctaaaagat
      361 agattgctga aatacagact ccacttttac atgcgcgaca aaaattactg ccttgatgaa
      421 aaaaccagaa agggaagaac atcactgcaa atacccaaaa aatagcaaag taataatgat
      481 cactggaaga cgctgattca ctttctgaag gattagtcgg atattggtgt tgtgaagtga
      541 gttggttatt caaagcgttt atagctagct ctaatccctg agctaatttt tgttgtttaa
      601 atgcgggtat catgttgcta cggatgatat ctccagccag agcatcggtt accttttctt
      661 ccagcccata acctaccttg atgcggacag tacgatctga ccaggcaaca atgatcagta
      721 tcccatcatt acgtttggca tctcctaaac gccaattgtc aaaaactctt gtagcatatt
      781 gttcaattgt ctcgtctttg gttgtgggaa caattaatac agcaatttgc acttgggtat
      841 tattgaaaat agattctaac ttgtgcgtca gttctttctt ttcttgtgag tttaagctac
      901 tcgtaagatc cgtaacttgt tgctgtaatt gtgggacaat agttggttgg gctgcaacaa
      961 aggaacacac gaaagt
//
LOCUS       DBANUU010000410          975 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.417, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000410 DBANUU010000000
VERSION     DBANUU010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.417"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(78..323)
                     /locus_tag="ACIE68_005069"
     CDS             complement(78..323)
                     /locus_tag="ACIE68_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxA family glutaredoxin"
                     /protein_id="HFX5911201.1"
                     /translation="MKVVIYGRDNCSYCKRAVELAKQLKGHGYGDYEYIDITTAGIDK
                     EKLSEIVDKPVETIPQVLIDGQPIGGYTELAAYVSTL"
     gene            complement(353..508)
                     /locus_tag="ACIE68_005070"
                     /pseudo
     CDS             complement(353..508)
                     /locus_tag="ACIE68_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812573.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation protein PhoH"
     gene            655..>975
                     /locus_tag="ACIE68_005071"
     CDS             655..>975
                     /locus_tag="ACIE68_005071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911202.1"
                     /translation="MPVLLILILGTVMIIFWDTVKENVEVIGTLATSLAFFATAWAAY
                     EARHSAKAAMRATRLTAASLLEMKKASFKEWYGILLEQHNRLLEDVNKTLQDDRELNA
                     KLGTN"
BASE COUNT          262 a          198 c          232 g          283 t
ORIGIN      
        1 cgtcgttttt taaattttaa ggtaaacagg agtgagtttg gtggggaata aaaaacggct
       61 cctgtgagcc gttaaaatca gagggtgctg acgtatgcag ccagttctgt gtatccgcca
      121 atcggctggc catcgatcag cacctggggg atagtctcta ccggtttatc aacaatttca
      181 cttagttttt ccttgtcgat accggcagtg gtgatgtcga tgtactcata atcaccgtag
      241 ccatgtccct ttagttgctt cgccagctca accgcacgtt tgcagtatga gcagttatct
      301 cgtccgtaga taacgacttt catcacttca cctcgtgcaa ctgctgttca ttaacgatga
      361 ttgtgtcaat ttcgtagccg tggatgcgtg gctttggctt cactgcctga cgagctgcgc
      421 gacgcgcctg tttacgtttg tttcccatat agagtccttt caagtgagta agtgtcgtga
      481 ctatagctaa aatgaatagg tgagtcatta cctatttaat gtgtttggtt actaagcaga
      541 aatggctttg tgaggttgac acattagaaa cggcaagaca gctttaaact agctgaaatg
      601 gtatggtcga ttaggtatca ttcagtttca aaattaacca aagacactga taaaatgcct
      661 gttttgttaa tcctcatact tgggacagtg atgattattt tctgggatac cgtcaaggaa
      721 aacgtagagg tcataggtac acttgccact tcacttgcat ttttcgccac agcatgggcc
      781 gcttacgagg caaggcatag tgcaaaggca gcaatgaggg ccactaggct gacggcagct
      841 tcactccttg aaatgaaaaa agcctctttt aaggaatggt acggcatatt acttgaacag
      901 cataatagat tacttgagga tgttaacaag accttgcagg atgataggga acttaatgca
      961 aaactgggaa cgaat
//
LOCUS       DBANUU010000411          974 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.418, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000411 DBANUU010000000
VERSION     DBANUU010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.418"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(207..878)
                     /gene="queE"
                     /locus_tag="ACIE68_005072"
     CDS             complement(207..878)
                     /gene="queE"
                     /locus_tag="ACIE68_005072"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFX5911203.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          266 a          240 c          208 g          260 t
ORIGIN      
        1 aaataactct atacaaatta aaatctacca aactttaccg caataatttt cactccagcg
       61 aaaaattaat gccacagaat ttgtagagaa tgctaatgat tagccctgga tgggtaaaat
      121 ggaggagttt tcagaggcga caaacaatat aaatgagtag aagtctttac aggttccacc
      181 catttataac gcttataaat gtttaatcag gcaatattta gatatttatg tgtttgcatc
      241 gacaaacgcc aattacgcgc aatgcaggtt tcaatgcaca aacgtgtggc atcatccttt
      301 tggctaatcg gctgcagtgc aatgactcgc ggtttatcat cggtcagcgt tgccagtagt
      361 tcatccagtg cttcaatatc gcgtacgcgc cccactggat gcttgatttc gttggctcgc
      421 tccagtgcct gtgacaacac ttcatagccg ccgcgcatgt tcagctttgg cgatacggta
      481 acccaggtat tcggtgtgca gcgtacctca tgagtaccac tggtttcgat ctggcagcta
      541 aaaccgttct tttcgagcag atcagtcagt ggcagcaaat catgaatgca aggctcacca
      601 cccgtaatca ccacatgccg cgcggtgtat ccctggcgac taatgacagc caacaagtct
      661 tcactgctcg ccgcccccca cttatcactc tctttggtct tcgccagaat gctgaaaagg
      721 gagacttccc gatcctcaag cttttcccag gtgtgtttgg tgtcgcacca ggcacagcca
      781 accgggcatc cctgtaaacg aataaaaatg gcggggacgc cggtaaagta accctcacct
      841 tgcagggtct ggaacatctc gttaatcggg tactgcatag cattctctgt gaagtggata
      901 attgttaatt attgcagatc ctgccacaac aatcatgtct tattaacatt ctgttacagg
      961 cagatttaaa gaaa
//
LOCUS       DBANUU010000412          968 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.419, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000412 DBANUU010000000
VERSION     DBANUU010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.419"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..211
                     /locus_tag="ACIE68_005073"
     CDS             <1..211
                     /locus_tag="ACIE68_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFX5911204.1"
                     /translation="MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRK
                     LLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(222..293)
                     /locus_tag="ACIE68_005074"
                     /pseudo
     CDS             complement(222..293)
                     /locus_tag="ACIE68_005074"
                     /inference="COORDINATES: protein motif:HMM:NF013446.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            353..502
                     /locus_tag="ACIE68_005075"
                     /pseudo
     CDS             353..502
                     /locus_tag="ACIE68_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            529..633
                     /locus_tag="ACIE68_005076"
                     /pseudo
     CDS             529..633
                     /locus_tag="ACIE68_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          313 a          192 c          200 g          263 t
ORIGIN      
        1 gatgacggat ggctggccgc tgtatgaatc acgcctgaag ggaaagctgc acgttatcag
       61 caagcgttac actcagcgca ttgagcgaca taatctgaat ctgagacaac atctggcaag
      121 gctgggacgg aagttactgt cgttctcaaa atcggtggag ttgcatgaca aggtcatcgg
      181 gcattatctg aacataaaac actatcagta agttggagtc attacctcct cccgaactca
      241 aagctatcat acagataatt actggttaac cctatatagg gcattttaaa gtgttgtgat
      301 cttacccagc aatagtggac acgcggctaa gtgagtaaac tctcagtcag aggtgactca
      361 caagacaaaa cagtttcaac cagtaaaaaa acgtaaacac cattcgcatg aatttcgtag
      421 ttaagatctg aagcttgcag aatgcatcgg tgttactgcc ggagtccgtg aacttagtct
      481 gcatgaatca ccacagcttt acatctgtcg cagtaaacag caaaatcagc aaacttactt
      541 aattatatcg agtgcgatta caatcgctgg cgatggtaca gttggtgtgg cggtctcagt
      601 ccggaacaat ttgaaaatca gaatctcact taaaacggca tccattttac aaaagatggc
      661 cgtctgtgta tttataatga ataccttcta catgtttaga gtgcataatc cacgacaagg
      721 cgatatccta aaaattagct agcaggcaac tttgatggca tagataatgt ttttagtaaa
      781 aaagagttga ctgaatataa tacagcctct tcctacataa gaaggaatgt gctcacaaaa
      841 aaaaccatta gtctgcttcc tgttcttaca ataatcagta ttatgtattt tatttatata
      901 gcaaaatatg cttgttacac ctgtcataat taggcatggt aaaattattg actgaaccct
      961 gggggaga
//
LOCUS       DBANUU010000413          957 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.420, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000413 DBANUU010000000
VERSION     DBANUU010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.420"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..899)
                     /locus_tag="ACIE68_005077"
     CDS             complement(<1..899)
                     /locus_tag="ACIE68_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFX5911205.1"
                     /translation="MATAWYKQVNPPQRKALFSAWLGYVFDGFDFMMIFYILHIIKAD
                     LGITDIQATLIGTVAFIARPIGGGFFGAMADKYGRKPMMMWAIFIYSVGTGLSGIATN
                     LYMLAVCRFIVGLGMSGEYACASTYAVESWPKNLQSKASAFLVSGFSVGNIIAAQIIP
                     QFAEVYGWRNSFFIGLLPVLLVLWIRKSAPESQEWIEDKYKDKSTFLSVFRKPHLSIS
                     MIVFLVCFCLFGANWPINGLLPSYLADNGVNTVVISTLMTIAGLGTLTGTIFFGFVGD
                     KIGVKKAFVVGLITSFIFLCPLF"
BASE COUNT          340 a          198 c          157 g          262 t
ORIGIN      
        1 aaaaaaagag gacaaaggaa aataaatgaa gttattagac cgactacaaa ggcttttttt
       61 acaccaatct tatcaccaac aaaaccaaaa aatattgtac ctgtcagtgt tcctaaacct
      121 gctattgtca tcagagttga aatgaccact gtattaactc cattatctgc caggtaggaa
      181 ggaagtagtc cgtttatcgg ccagtttgca ccaaatagac aaaaacagac gaggaaaacg
      241 atcatagaga ttgaaagatg tggttttctg aagacagaca aaaatgttga tttatcctta
      301 tatttatctt caatccactc ctgactttct ggagcacttt ttctgatcca aagaactagt
      361 aaaactggta acaggcctat aaaaaaagag tttctccatc catatacttc agcaaactga
      421 gggattattt gtgccgcaat aatatttcca acagaaaaac cacttaccaa aaaagcacta
      481 gctttagatt gaagattttt aggccaactt tctaccgcat aagttgaagc acatgcatat
      541 tcaccagaca tccctaagcc aacaataaaa cggcaaactg cgagcatata taagtttgta
      601 gcaataccgc taaggcctgt tccgactgag taaatgaaaa ttgcccacat catcattggc
      661 ttacgaccat atttatcagc catggcacca aaaaaaccac ctccaatagg tctggctatg
      721 aaggccactg tccctattaa agtagcctga atatccgtaa tgccaagatc tgcttttata
      781 atatgaagaa tgtaaaatat catcataaaa tcaaagccat caaatacata tccaagccat
      841 gcggaaaaaa gagctttccg ttgtggtgga ttaacttgtt tataccatgc tgttgccata
      901 tttgtttcct gtgttattct aatatagccc ttaaagtaaa tattagggag cctcaac
//
LOCUS       DBANUU010000414          955 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.421, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000414 DBANUU010000000
VERSION     DBANUU010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.421"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..627
                     /gene="traU"
                     /locus_tag="ACIE68_005078"
     CDS             <1..627
                     /gene="traU"
                     /locus_tag="ACIE68_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFX5911206.1"
                     /translation="DEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLDIAYLSE
                     IDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPIDALFWCAGSQGSMYP
                     FNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILPKERWRY
                     QMVNMYPDSGQCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            636..>955
                     /locus_tag="ACIE68_005079"
     CDS             636..>955
                     /locus_tag="ACIE68_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFX5911207.1"
                     /translation="MKLSMKSLAALLMMLNGAVMASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDEVVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKR"
BASE COUNT          245 a          244 c          260 g          206 t
ORIGIN      
        1 gatgagaagc aggtgaacgg ggcgttctat cacgttcact ggtacaaata cccgctgacg
       61 tactggctta acatcatcac atcgctgggc tgtctggaag gtggtgacct ggatatcgct
      121 tatctttctg aaatcgaccc cacctggacg gacagcagcc tgaccaccat tctcaatccg
      181 gaagctgtca tctttgccaa tccgatagca cagggagcct gcgcagcaga tgcgattgcc
      241 agcgccttta acatgcccat cgatgctctg ttctggtgtg ccggttcgca gggaagtatg
      301 tacccgttca atggctgggt gagtaatgag tccagtccgt tgcagtcctc cctgctggtc
      361 agtgaacgca tggcgttcaa gctgcaccgt cagggcatga ttatggaaac catcgggaaa
      421 aataacgccg tctgtaatga atatccgtcc ccaatcctgc ccaaagaacg ctggcgttac
      481 cagatggtga atatgtatcc ggacagcgga cagtgccacc cgttcgggcg cagcgtgacc
      541 cgctgggaaa ccgggaaaaa tccacccaac acaaagaaaa acttcggcta cctgatgtgg
      601 cgtaaacgta actgtgtatt cctgtgaggt gaatgatgaa gctgagtatg aaatctctgg
      661 cagcactgct gatgatgctg aacggggcgg ttatggcgtc agaaaacgtg aacactcctg
      721 aaaaccgcca gttcctgaag cagcaggaaa atttaagcag acaactgcgt gaaaaacctg
      781 accatcagct gaaagcctgg gcggagaaac aggtgctgga aaaccccctt cagcgttcag
      841 ataaccattt cctggatgaa gtggttcgta aacagcaggc ttcgcaggac gggaaacccc
      901 ggcagggtgc cctgtatttt gtgtcgtttt ccattcccga agaggggctg aaacg
//
LOCUS       DBANUU010000415          945 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.422, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000415 DBANUU010000000
VERSION     DBANUU010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.422"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..617
                     /gene="traU"
                     /locus_tag="ACIE68_005080"
     CDS             <1..617
                     /gene="traU"
                     /locus_tag="ACIE68_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFX5911208.1"
                     /translation="VNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLDIAYLSEIDPT
                     WTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGSQGSMYPFNGW
                     VSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILPKERWRYQMVN
                     MYPDSGQCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            626..>945
                     /locus_tag="ACIE68_005081"
     CDS             626..>945
                     /locus_tag="ACIE68_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFX5911209.1"
                     /translation="MKLSMKSLAALLMMLNGAVMASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKR"
BASE COUNT          235 a          242 c          257 g          211 t
ORIGIN      
        1 aggtgaacgg ggcgttctat cacgttcact ggtacaaata cccgctgacg tactggctta
       61 acatcatcac atcgctgggc tgtctggaag gtggtgacct ggatatcgct tatctttctg
      121 aaatcgaccc cacctggacg gacagcagcc tgaccaccat tctcaatccg gaagctgtca
      181 tctttgccaa tccgatagca cagggggcct gtgcagcaga tgccattgcc agcgccttta
      241 atatgcctct cgatgttctg ttctggtgtg ccggttcgca gggcagtatg tacccgttca
      301 atggctgggt gagtaatgag tccagtccgt tgcagtcctc cctgctggtc agtgaacgca
      361 tggcgttcaa gctgcaccgt cagggcatga ttatggaaac catcgggaaa aataacgccg
      421 tctgtaatga atatccgtcc ccaatcctgc ccaaagaacg ctggcgttac cagatggtga
      481 atatgtaccc ggacagcgga cagtgccacc cgttcgggcg cagtgtgacc cgctgggaaa
      541 ccgggaaaaa tccgcccaat acaaagaaaa acttcggcta cctgatgtgg cgtaaacgta
      601 actgtgtctt cctgtgaggt gaatgatgaa gctgagtatg aaatctctgg cagcactgct
      661 gatgatgctg aacggggcgg ttatggcgtc agaaaacgtg aacactcctg aaaaccgcca
      721 gttcctgaaa cagcaggaaa atttaagcag acaactgcgt gaaaaacctg accatcagct
      781 gaaagcctgg gcggagaagc aggtgctgga aaaccccctt cagcgttcag ataaccattt
      841 cctggatgag ctggttcgta aacagcaggc ttcgcaggac gggaaacccc ggcagggtgc
      901 tctgtatttt gtgtcgtttt ccattcccga agaggggctg aaacg
//
LOCUS       DBANUU010000416          933 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.423, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000416 DBANUU010000000
VERSION     DBANUU010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.423"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..125)
                     /locus_tag="ACIE68_005082"
     CDS             complement(<1..125)
                     /locus_tag="ACIE68_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001359473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFX5911210.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIR"
     gene            complement(155..766)
                     /locus_tag="ACIE68_005083"
                     /pseudo
     CDS             complement(155..766)
                     /locus_tag="ACIE68_005083"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            complement(772..>933)
                     /locus_tag="ACIE68_005084"
     CDS             complement(772..>933)
                     /locus_tag="ACIE68_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFX5911211.1"
                     /translation="DECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGG
                     FLSINKLSQ"
BASE COUNT          206 a          298 c          238 g          191 t
ORIGIN      
        1 cggatggctt cagcccccgt tttcacacga aggtcgatgc ggcgaccaaa tcgttgtaaa
       61 tccccgtaaa ggcagatgcg cgccatgccc ggtgacgcca gagggagtgt gtgcgtcgct
      121 gccatttgtc ggtgtacctc tctcgtttgc tcagttgttc aggaatatgg tgcagcagct
      181 cgccgtcgcc gcagtaaatt gcggcgtgat tcggcaccga tgaaccaaaa cagcacagca
      241 gcacatcgcc cggctgtgcc gctgacaacg gcacctgata cagccccgtc gcctccagat
      301 tatccagata gagattctgg ccgttacgcc accagtcatc ctcacgatga aaatccggca
      361 tctcaatccc cgccagatga taagcatccc ggaacagtgt gtaacagtcc gtcacaccgt
      421 gctcaaagcg ccgccaggtg agatgcggca cacagcggaa cttatgaatc gtcccccggc
      481 agaccagcca ccacggcaaa tcactctgca cctgcagccg ccggtcggcc tcactcagcc
      541 agggcagacc accggggtgg ctgtggacca gcgccacaat ctcaccctgc atttctgcct
      601 gcagccagtc ttccggcgac atccggaaat aatcctccgg ctcaccggag atattcacgc
      661 agggaaaata tctttccccc tccggtgttc tcaccacgaa gccgcacgac tccgctggtg
      721 cacatcgccg ggcgtgcgcc agaatcgctg attctgtctg tgtcatggga ttcactgcga
      781 aagtttgtta atggaaagga agccgccaaa gttgccgacg ttattgcgaa acttacagcc
      841 gctcaggcat ttgctgcatt tatccttcgt gatatcggac gtcggctgat catattcatc
      901 cgcaactgcc ggaccgtgat aaccgcactc atc
//
LOCUS       DBANUU010000417          928 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.424, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000417 DBANUU010000000
VERSION     DBANUU010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.424"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>928
                     /locus_tag="ACIE68_005085"
     CDS             <1..>928
                     /locus_tag="ACIE68_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFX5911212.1"
                     /translation="AGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGR
                     VIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYD
                     EADRLTHRTVKGETAEQWQYDERGWLTDISHISEGHRVTVHYGYDEKGRLTGERQTVH
                     HPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGYLAGMKLGDTPLVEYT
                     RDRLHRETLRSFGRYELTTAYTPAGQLQSQHLNSLLSDRDYTWNDNGELIRISSPRQT
                     RSYSYSTTGRLTGVHTTAANLDIRIPYATDPAG"
BASE COUNT          221 a          302 c          274 g          131 t
ORIGIN      
        1 ccgccggtga cctgaccacc gtcattgccc cggacggcag cagaaacggg acacagtacg
       61 atgcgtgggg aaaagccatc tgtaccacgc agggcggtct gacgcgcagt atggaatacg
      121 atgctgccgg acgggtcatc cgcctgacca gtgaaaacgg cagccacacc accttccgtt
      181 acgatgtact cgaccggctg atacaggaaa ccggctttga cggccgcaca cagcgttatc
      241 accacgacct gaccggcaaa cttatccgca gcgaggatga ggggctggtc acccactggc
      301 actatgacga agcagaccgc ctcacgcacc gcaccgtgaa gggtgaaacc gcagagcagt
      361 ggcagtatga cgaacgcggc tggctgacag acatcagcca tatcagcgaa gggcaccggg
      421 tgacggtgca ttacgggtat gatgagaaag gccggctgac cggtgagcgt cagacggtgc
      481 atcacccgca gacggaagca ctgctctggc agcatgagac cagacatgcg tacaacgcgc
      541 aggggctggc gaaccgctgt ataccggaca gcctgcccgc cgtggaatgg ctgacctacg
      601 gcagcggtta cctggcaggc atgaaactcg gcgacacacc gctggtggag tacacccgcg
      661 accgcctgca ccgggaaacg ctgcgcagct tcggccgtta tgaactcacc accgcttata
      721 cccctgccgg gcagttacag agccagcacc tgaacagcct gctgtctgac cgcgattaca
      781 cctggaacga caacggcgaa ctcatccgca tcagcagccc gcgccagacc cggagttaca
      841 gctacagcac caccggcagg ctgaccggcg ttcacaccac cgcagcgaat ctggatatcc
      901 gcatcccgta tgccacagac ccggcagg
//
LOCUS       DBANUU010000418          928 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.425, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000418 DBANUU010000000
VERSION     DBANUU010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.425"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>928
                     /locus_tag="ACIE68_005086"
     CDS             <1..>928
                     /locus_tag="ACIE68_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFX5911213.1"
                     /translation="AGDLTAVIAPDGSRNGTQYDAWGKAVRTTQGGLTRSMEYDAAGR
                     VIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYD
                     EADRLTHRTVKGETAERWRYDERGWLTDISHISEGHRVAVHYRYDEKGRLTGERQTVH
                     HPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGWLAGMKLGDTPLVDFT
                     RDRLHRETLRSFGRYELTTAYTPAGQLQRQHLNSLQYDRDYTWNDNGELIRISSPRQT
                     RSYSYSTTGRLTGVHTTAANLDIRIPYATDPAG"
BASE COUNT          214 a          301 c          281 g          132 t
ORIGIN      
        1 tcgccggtga cctgaccgcc gtcattgccc cggacggcag cagaaacggg acacagtacg
       61 atgcgtgggg aaaggccgtc cgtaccacgc agggcgggct gacgcgcagt atggaatacg
      121 atgctgccgg acgggtcatc cgcctgacca gtgaaaacgg cagccacacc accttccgtt
      181 acgatgtact tgaccggctg atacaggaaa ccggctttga cggccgcaca cagcgttatc
      241 accacgacct gaccggcaaa cttatccgca gcgaggatga ggggctggtc acccactggc
      301 actatgacga agcagaccgc ctcacgcacc gcaccgtgaa gggtgaaacc gcagagcggt
      361 ggcggtatga cgaacgtggc tggctgacag acatcagcca tatcagcgaa gggcaccggg
      421 tagcggtgca ttacaggtat gatgagaaag gccgcctgac cggtgagcgc cagacggtgc
      481 atcacccgca gacggaagca ctgctctggc agcatgagac cagacatgcg tacaacgcgc
      541 agggactggc gaaccgctgt ataccggaca gcctgcccgc cgtggaatgg ctgacctacg
      601 gcagcggctg gctggcaggc atgaagctcg gcgacacacc gctggtggat ttcacccgcg
      661 accgcctgca ccgggaaacg ctgcgcagct tcggtcgtta tgaactcacc accgcttaca
      721 cccctgccgg gcagttacag cgtcagcacc tgaacagcct gcagtatgac cgcgattaca
      781 cctggaacga caacggcgaa ctcatccgca tcagcagccc gcgccagacc cggagttaca
      841 gctacagcac caccggcagg ctgaccggcg ttcacaccac cgcagcgaat ctggatatcc
      901 gcatcccgta tgccacggac ccggcggg
//
LOCUS       DBANUU010000419          923 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.426, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000419 DBANUU010000000
VERSION     DBANUU010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.426"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..749
                     /locus_tag="ACIE68_005087"
     CDS             <1..749
                     /locus_tag="ACIE68_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911214.1"
                     /translation="PNDQLRGQLSDYFNRCLPDSPKKKERESAADLTIKNYPSFIDYY
                     IRYKEQNSGGAKRDSNAKVSETEQRYIEAVQLLVDTIFNKHQEFFSSKDDTFEEAYKR
                     VCYLKQVIENNDGYKVFYLNGVPIKREADLQLMFRLTWFASISDVNSEVNNGRGPVDY
                     KISRGSKDKTLVEFKLASNSKLKQNLANQVKVYEAANETKKSIKVILYFTDRELSGLF
                     KTFKELGIKEGKDLVLIDARPNKVSASNVK"
BASE COUNT          305 a          123 c          195 g          300 t
ORIGIN      
        1 tccctaatga tcaattgcgt ggacaattgt ctgattattt caatagatgt ctcccagact
       61 ctccaaagaa aaaggaacga gagtccgctg ctgatttgac aattaagaat tacccgagtt
      121 ttattgatta ttatattcgt tataaagaac aaaactctgg gggtgctaaa cgtgatagta
      181 atgcaaaggt tagcgaaact gagcaaaggt atattgaagc tgtccaactt ttggttgata
      241 caatctttaa taaacatcag gagttcttta gttcaaagga tgatactttc gaagaggcct
      301 ataagcgagt ctgttatctt aaacaggtaa tagaaaataa tgatggctat aaagtattct
      361 atttgaatgg tgtcccaatt aagcgtgaag ctgatttgca gttaatgttt agattaacat
      421 ggtttgctag tatttctgat gtcaatagtg aagtaaataa tggtcgtggt cccgttgact
      481 acaaaatatc gagaggttcg aaagataaaa cattggttga attcaaactg gctagtaact
      541 ccaagcttaa acagaatctg gcaaatcagg ttaaagtgta tgaggcggca aatgaaacaa
      601 aaaaatcaat aaaggttatt ttgtatttta ctgatcgaga attgtctgga ttgtttaaga
      661 ctttcaaaga acttgggatt aaagaaggta aggatttagt cttgattgat gctaggccta
      721 ataaggtttc tgcatcgaat gtcaaataac ttgttgggaa tgagtatgat ttaagccgca
      781 ttatggcggc tttttataat cttcttttat tatggtaacg acactttaga tgtatagggc
      841 agtattcatt ttaataaaca gttttgcaca tgagcgtgat ggtattaagt atttgtgaac
      901 tttattaagt atgtaacaag gtc
//
LOCUS       DBANUU010000420          922 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.427, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000420 DBANUU010000000
VERSION     DBANUU010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.427"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..842
                     /locus_tag="ACIE68_005088"
     CDS             <1..842
                     /locus_tag="ACIE68_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFX5911215.1"
                     /translation="SMPLSNTLQSQIITDNHFLHHPKVDSELTRKYERARLDTENIYL
                     LPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKIT
                     SALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYAG
                     TTIGYISTLPTSQAKRWTNEQPRIDIYIDQIMTVSGVANSSGFALAALLNANIELGND
                     PIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYMVML
                     PTY"
BASE COUNT          281 a          187 c          193 g          261 t
ORIGIN      
        1 tgtctatgcc attaagcaat acccttcaga gccaaattat tacagataat cacttccttc
       61 atcatccaaa agtcgacagt gagttaacac gtaaatatga acgtgcacgc ctggatacgg
      121 aaaatatcta tcttctgcct cttgctcgtg gcaataacca taattacgat ggaaaaagcg
      181 ttgtcgaaat tagaaaactt gatattagta aagagtcttg gccatttaat tatgtgacag
      241 aagcatgcag agaatccgat ggtattacaa caacaggacg tatgctctat cgaaacttga
      301 aaatcacctc agctttagac gaaatatatg gtggcatatg taaaaaggcg catgctgcta
      361 cagaattagc agaaggttta cggctaaatt tatttatgaa gagccctttc gatcctgttg
      421 aggactatac agttcatgaa ataacattag gccctggttg caatgttcca ggttacgccg
      481 gaaccacgat aggatatatt tcaactcttc caacttctca ggcaaaaaga tggaccaatg
      541 agcagccaag gattgatatc tatattgatc agataatgac tgtctcaggg gtggcaaact
      601 cttccggatt cgcccttgcc gcattactta atgctaatat tgagttgggt aatgacccaa
      661 taattggtat agaagcctat cccggtaccg cggagatcca tgcaaaaatg ggatataaag
      721 ttatcccggg tgatgaaaat gcaccactca aacgtatgac tctgcaaccg tcatctcttc
      781 cagagctgtt tgaattaaaa aatggtgaat ggaattatat ggtgatgctg ccaacttact
      841 gatttagcgt atgatggtgt ttttgaggtg ctccagtggc ttctgtttct atcagctgtc
      901 cctcctgttc agctactgac gg
//
LOCUS       DBANUU010000421          910 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.428, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000421 DBANUU010000000
VERSION     DBANUU010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.428"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..310
                     /locus_tag="ACIE68_005089"
     CDS             <1..310
                     /locus_tag="ACIE68_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911216.1"
                     /translation="IKTSEYPEEFFSNAVWGKKNIKPGEYYSVQAVSMFTTNQFILLC
                     PKDIIRFYRESWKFDLLNVDFDTNTRKKLQEEAAYCVPISAISVKRWDMPLQGSKPSN
                     "
     gene            complement(536..711)
                     /locus_tag="ACIE68_005090"
                     /pseudo
     CDS             complement(536..711)
                     /locus_tag="ACIE68_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          282 a          183 c          191 g          254 t
ORIGIN      
        1 tattaaaaca agtgaatatc cggaggagtt tttttccaat gcagtatggg gtaaaaaaaa
       61 tataaaaccg ggggaatatt actctgtgca ggctgtttct atgtttacga caaaccagtt
      121 tattctcctt tgtcccaagg atattataag gttttatcgg gaaagctgga agttcgacct
      181 gcttaatgta gacttcgata caaataccag gaagaaactc caggaagaag cagcatattg
      241 cgtaccaatt tctgcaatat ctgtaaaacg atgggatatg cctcttcagg gctcgaaacc
      301 ctcaaattag aaggccgctc gtgcgactgt attagatagt aatgcggtat ggaaaaatag
      361 tgatggcaag atgaacttga cttggctaat caaataccgg cgtagtgatt aggaaacagg
      421 gctcggataa cattattgcg cggttcattg aacctactga tgacgccgga tatatagtcg
      481 taaaccgttt cgaaccagta cttatatcac caggagagtc catatatgac caccgtttac
      541 agtaaaaacg gtatctgttt tctgatccct ccagcgtcaa actaccgttg taatggtgag
      601 gtctggtgct attgtaataa ttcagaatat aatctctgat ttatttccga acctcgccct
      661 tgcctgcgta accatccgtc gcccccatcc tgttttcaga ctgcggaaaa aggcaagttt
      721 ttatcccccc acagagattc aatcatatat cgcgtgtttt ggaagtgaca gttcttattc
      781 ctgagaatac agcatgtgca acacactcgg catccatcga taccactttt atctctaata
      841 ggcaggataa aaaacaagac tgtgaattaa agtaatcggc aaatgaatga gttagaagat
      901 actgaaaatg
//
LOCUS       DBANUU010000422          910 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.429, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000422 DBANUU010000000
VERSION     DBANUU010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.429"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..804
                     /gene="gadX"
                     /locus_tag="ACIE68_005091"
     CDS             <1..804
                     /gene="gadX"
                     /locus_tag="ACIE68_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFX5911217.1"
                     /translation="CLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENF
                     VLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQNKNLRYA
                     EMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASE
                     LLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFI
                     YVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          263 a          175 c          207 g          265 t
ORIGIN      
        1 tgtctaattg cgtacgcaag acataaatat attctcacca tggttaatgg tgaatatcgc
       61 tattttaatg gcggtgacct ggtttttgcg gatgcaagcc aaattcgagt agataagtgt
      121 gttgaaaatt ttgtattagt atcaagggat acgctttcat tatttctgcc gatgctcaag
      181 gaggaggcat taaatcttca tgcacataaa aaaatttctt cattactcgt tcatcactgt
      241 agcagagata ttcctgtttt tcaggaagtt gcgcaactat cgcagaataa gaatcttcgc
      301 tatgcagaaa tgctacgtaa aagagcatta atctttgcgt tgttatctgt ttttcttgag
      361 gatgagcact ttataccgct gcttttgaac gttttgcaac cgaacatgcg aacacgagtt
      421 tgtacggtta tcaataataa tatcgcccat gagtggacac tagcccgaat cgccagcgag
      481 ctgttgatga gtccaagcct gttaaagaaa aaattgcgcg aagaagagac atcatattca
      541 cagttgctta ctgagtgtag aatgcaacgt gctttgcaac ttattgttat acatggcttt
      601 tcaattaagc gagtcgcagt gtcctgtgga tatcacagcg tgtcgtattt catttacgtc
      661 tttcgaaatt attatgggat gacgcccaca gagtatcagg agcgatcggc gcagggattg
      721 ccgaaccgtg actcggcggc aagtattgtt gcgcaaggaa atttttacgg cactgaccgt
      781 tctgcggaag gaataagatt atagaatttt actcagacat aaaaaaaacc cggcataggg
      841 gaccgggaag aggatagtct gccgtctcca gactaataaa ccgttataac actccctgtt
      901 ggcacgggaa
//
LOCUS       DBANUU010000423          908 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.430, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000423 DBANUU010000000
VERSION     DBANUU010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.430"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..513
                     /locus_tag="ACIE68_005092"
     CDS             <1..513
                     /locus_tag="ACIE68_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFX5911218.1"
                     /translation="AGDTSFTLAWMPGEQGQQALLAWFNEGDTRAYKIRFPNGTVDVF
                     RGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTAATGMTVTPASTSVVKGQS
                     TTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKGVAAGKVNIPVVSGNGELA
                     AVAEITVTAS"
     gene            529..>908
                     /locus_tag="ACIE68_005093"
     CDS             529..>908
                     /locus_tag="ACIE68_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFX5911219.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTKLPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGP"
BASE COUNT          223 a          233 c          277 g          175 t
ORIGIN      
        1 gccggagata ccagcttcac gctggcgtgg atgcccggag agcaggggca gcaggcgctg
       61 ctggcgtggt ttaatgaagg tgatacccgt gcctataaaa tccgcttccc gaacggcacg
      121 gtcgatgtgt tccgtggctg ggtcagcagt atcggtaaag cggtgacggc gaaggaagtg
      181 atcacccgca cggtgaaagt caccaacgtg ggacgtccgt caatggcaga agatcgcagc
      241 acggtaacag cggcaaccgg catgaccgtg acgcctgcca gcacttcggt ggtgaaaggg
      301 cagagcacca cgctgaccgt ggcattccag ccggaaggcg caaccgacaa gagcttccgt
      361 gcggtgtctg cggataaaac aaaagccacc gtgtcggtca gtggtatgac catcaccgtg
      421 aaaggtgttg ctgcaggcaa ggtcaacatt ccggttgtat ccggtaatgg tgaacttgct
      481 gcggttgcag aaattaccgt caccgccagt taatccggag agtcagcgat gttcctgaaa
      541 accgaatcat ttgaacataa cggtgtgacc gtcacgcttt ctgaactgtc agccctgcag
      601 cgtattgagc atctcgccct gatgaaacgg caggcagaac aggcggagtc agacagcaac
      661 cggaagttta ctgtggaaga cgccatcaga accggcgcgt ttctggtggc gatgtccctg
      721 tggcataacc atccgcagaa gacaaagctg ccgtccatga atgaagccgt taaacagatt
      781 gagcaggaag tgcttaccac ctggcccacg gaggcaattt ctcatgctga aaacgtggtg
      841 taccggctgt ccggtatgta tgagtttgtg gtgaataatg ctcctgaaca gacagaggac
      901 gccgggcc
//
LOCUS       DBANUU010000424          907 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.431, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000424 DBANUU010000000
VERSION     DBANUU010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.431"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            93..860
                     /locus_tag="ACIE68_005094"
     CDS             93..860
                     /locus_tag="ACIE68_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HFX5911220.1"
                     /translation="MQFIHNDLGQRKRGEIVEITLTSGANVRLMDSSNFSNYKNGRRH
                     SYIGGLAKRSPIRLQIPSSGHWHIAVDMQGLRGSTRASVRVLPGVLPEIQDRPLSEVP
                     SLVRDNKPPSTAQSDEIHDVFISHASEDKDDFVRSLANSLISHGLNVWYDEMTLRIGD
                     SLRQKIDKGLANSRVGLVVLSPAFISKGWTNYELDGIITRAISGEQILLPIWHNITKQ
                     EVIDFSPSLADKVARSTATHTIDEIAHEIAELLHSRC"
BASE COUNT          284 a          176 c          195 g          252 t
ORIGIN      
        1 ttttcactct aggagatact ctctaccacc ggatacagta taattaggaa gtggctacat
       61 tcttccaatt caaatgaaga gaatagattt acatgcaatt catccataat gatcttggtc
      121 aaagaaaaag aggagagatt gttgaaatta ccctcaccag tggtgcaaat gttagactaa
      181 tggatagttc taacttcagt aactataaga atggccgacg acattcctac attggtgggt
      241 tagcaaagcg ctcccccata aggttacaga tcccttcatc aggccattgg catattgctg
      301 tagatatgca gggattaagg gggagtacta gggcttctgt tcgcgttctc ccgggggttc
      361 ttcctgaaat tcaggatagg ccgttaagcg aagttcctag tctggtaaga gataacaaac
      421 caccgtcaac agcacagagc gacgagatac acgacgtatt tatttcacat gcctcagagg
      481 ataaagatga ttttgttcgc tctctggcaa actcattaat cagtcacggc cttaacgtat
      541 ggtatgacga aatgacgtta agaattggtg atagcctgag acaaaaaata gacaaaggtt
      601 tagcaaacag tcgtgttggt ttagtagttt tgtctccggc gtttataagc aaaggttgga
      661 caaattatga acttgatgga atcatcactc gtgctatttc tggtgaacaa atattgcttc
      721 ctatatggca taatattaca aaacaagaag ttatcgattt tagcccatca ttagcagaca
      781 aagttgctcg tagtacggca actcatacaa tcgatgaaat tgctcatgag atagccgagc
      841 tactgcattc tcgttgttaa tgtttatcga cgctcaaata gcaaaacaaa aggatcaatc
      901 agcggtc
//
LOCUS       DBANUU010000425          899 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.432, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000425 DBANUU010000000
VERSION     DBANUU010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.432"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..612
                     /locus_tag="ACIE68_005095"
     CDS             <1..612
                     /locus_tag="ACIE68_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 34 domain-containing
                     protein"
                     /protein_id="HFX5911221.1"
                     /translation="NQLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLRGRYI
                     TQDPIGLKGGWNFYQYPLNPVTNTDPLGLEVFPRPFPLPIPWPKSPAQQQADDNAAKA
                     LTKWWNDTASQRIFDSLILNNPGLALDITMIASRGNVADTGITDRVNDIINDRFWSDG
                     KKPDRCDVLQELIDCGDISAKDAKSTQKAWNCRHSRQSNDKKR"
     gene            633..>899
                     /locus_tag="ACIE68_005096"
     CDS             633..>899
                     /locus_tag="ACIE68_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HFX5911222.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCED"
BASE COUNT          302 a          170 c          206 g          221 t
ORIGIN      
        1 aaccagctga atgaagagaa cccgcaccag ctgcagcagc tcatccgcct gccggggcag
       61 cagtatgatg aggagtccgg cctgtattat aaccgccacc gctattatga cccgctgcgg
      121 gggaggtata tcactcagga tccgattggg ctgaaggggg gatggaattt ttatcagtat
      181 ccgttgaatc cagttacgaa tacagatcct ctggggttag aagtttttcc tagaccattc
      241 cccttgccaa ttccatggcc caaaagccct gcacagcagc aagcagatga taatgctgca
      301 aaagcattga caaaatggtg gaacgataca gcatcacaaa gaatatttga ctctctaata
      361 ttgaataatc cgggactagc attagatata acaatgatag cttctcgtgg aaatgttgca
      421 gacacaggga taactgatcg tgtcaatgac ataataaatg acagattctg gagtgatggg
      481 aaaaaacccg acagatgtga cgtacttcag gaactaattg attgtggtga tattagtgct
      541 aaagatgcaa aaagcacaca gaaagcctgg aattgtcgtc actccagaca gtcaaacgat
      601 aaaaaaagat agcccttgtg gaggttcctg caatgtcaaa tacataccag aaaagaaagg
      661 caagtaaaga gtatggttta tataatcaat gtaagaaact aaatgatgat gaattatttc
      721 gcttacttga tgatcacaat tccttgaaaa ggatttcatc tgccagagta ttacagttaa
      781 gaggtgggca agacgctgtt agattggcaa ttgagttctg ctctgataaa aattatatcc
      841 gtagagatat cggagcattt atactcgggc aaataaaaat ttgcaaaaaa tgcgaagat
//
LOCUS       DBANUU010000426          875 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.433, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000426 DBANUU010000000
VERSION     DBANUU010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.433"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>875
                     /locus_tag="ACIE68_005097"
     CDS             <1..>875
                     /locus_tag="ACIE68_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFX5911223.1"
                     /translation="IIFYQPWVNALPPMPRHANPEQLEKTVRYLTQTVHPRSADNIDN
                     LNRSAEYIKEVFVSSGARITSQDVPITGGPYKNIVADYGPADGPLIIIGAHYDSASSY
                     ENDELTYTPGADDNASGVAGLLELARLLHQQVPKTGVQLVAYASEEPPFFRSDEMGSA
                     VHAASLERPVKLMIALEMIGYYDSAPGSQDYPYPAMSWLYPDRGDFIAVVGRMQDINA
                     VRQVKATLLSSRYLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAVMITDTAFYRNKQYH
                     LPGDTADRLNYQKMA"
BASE COUNT          230 a          212 c          201 g          232 t
ORIGIN      
        1 attatttttt accaaccctg ggtcaatgca ttaccgccaa tgccccgaca cgcaaatcct
       61 gagcaattag aaaaaacagt tcgttatctt acacaaactg tgcatccacg tagtgccgac
      121 aatattgata atctgaatag gtccgcagag tatataaaag aggtcttcgt cagtagcggt
      181 gccagaatta cctcgcagga cgtccccatt acgggtggcc cctacaaaaa cattgttgct
      241 gattatggtc ctgccgatgg accgctgatt attattggtg cgcactatga ctctgccagc
      301 agttatgaaa acgatgaatt gacctataca cctggcgcgg atgataacgc cagcggcgtg
      361 gcaggattac tcgaactggc acgtttgtta catcagcaag taccgaaaac aggcgtgcag
      421 ctggtcgcct atgcgtcgga agaaccgccc ttctttcgta gcgatgaaat ggggagcgcg
      481 gtacatgcag cttcgcttga gcgtccagta aaattaatga tagcactgga gatgattggc
      541 tattacgact ctgcgcctgg tagccaggat tacccttacc cggcaatgtc ctggctttat
      601 cccgatcggg gagactttat tgccgtggtc ggcagaatgc aggatatcaa cgccgttcgt
      661 caggtaaaag cgacattgtt atcatcccgg tatttatctg tttattctat gaatgcccca
      721 gggtttattc ccggtattga tttctctgat caccttaatt attggcaaca cgatattccc
      781 gccgtaatga ttactgacac cgctttttat cgtaataaac aataccactt gcccggtgat
      841 accgcagaca gattgaatta tcagaaaatg gctca
//
LOCUS       DBANUU010000427          873 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.434, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000427 DBANUU010000000
VERSION     DBANUU010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.434"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(477..719)
                     /locus_tag="ACIE68_005098"
     CDS             complement(477..719)
                     /locus_tag="ACIE68_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFX5911224.1"
                     /translation="MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHN
                     QLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP"
BASE COUNT          221 a          215 c          187 g          250 t
ORIGIN      
        1 ttattgcttg aaaacagcaa gttatttgat ggcttgctca ttttttaatc cgcgccagcg
       61 gatagaaggg gcgaaaaaaa ataccaggga gcagcggggc cacatcgccc cgaagcgcgg
      121 cgcagccgct gacgaacata ccaggatgca cagggccatc ccttcgccct gcaaagccag
      181 aacagaccaa caggctatga tcatgctgat ttgcgccgca gaatgccgca gttagcgcga
      241 gcgccagcga gttcacgccg tcctggtgca ttgcagcagg gcggggcgct gtactgcgcg
      301 tgtgagcgtc agcgagctac gggcgcaaca acacaccagg caaaatcgca attatgtaaa
      361 gtcgccctgt tacaaacaca cactgtgtaa agacaaagaa caattacaat gtaaagacaa
      421 ataaaccttt acactgtata tcttagattt ctgcgtcgtg gtgtttttct tctcgctcac
      481 ggttcctccg gtgagcggga ttcagctttt ttatgcagga cgtattcagg gtgctcccct
      541 gcaagtaata acaggtactc aaattcacct ttcgataaat taagctggtt gtgggtttta
      601 gcttcttttt tctgccatgt acttaagcct acgccaaaaa tctcaccgca ctctgtctgg
      661 gtaaggccaa tttttttacg taatttcttt atgttttccg gtgtattttc gatagtcatg
      721 catcactctc cttatattct ttcaccatag ttaagagaat attcttatca acaggtctta
      781 aaccattttt tgtaaagtct attgcctcga cctcactttc attaaaaacc agctcactta
      841 acgttctctt tgttcttttt gtttttccgt cag
//
LOCUS       DBANUU010000428          872 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.435, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000428 DBANUU010000000
VERSION     DBANUU010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.435"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            163..765
                     /gene="fimB"
                     /locus_tag="ACIE68_005099"
                     /pseudo
     CDS             163..765
                     /gene="fimB"
                     /locus_tag="ACIE68_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
BASE COUNT          257 a          154 c          187 g          274 t
ORIGIN      
        1 aattaactgt tgctactcca tttaagttca ctcagaagaa ctggttcact tacgttagtt
       61 attaagcaaa cgttctcttt tataaacata atcaggatta aaatgttgga ttattgctaa
      121 cccagcacag ctagtgcgcg tctgtaatta taagggaaaa cgatgaagaa taaggctgat
      181 aacaaaaaaa ggaacttcct tacccatagt gaaatcgagt cactccttaa agcagcaaat
      241 accgggcctc atgcagcacg taattattgt ctgactttgc tttgttttat tcatggtttc
      301 cgggcgagtg aaatttgtcg attgaggatt tcggatattg atcttaaggc aaagtgtata
      361 tatatccatc gattaaaaaa aggcttttca acaacgcacc cgctattgaa caaagaagtt
      421 caggctttaa aaaactggtt gagtatacgt acttcttatc cgcatgctga gagcgagtgg
      481 gtatttttat cacgtaaggg gaatccgctt tctcggcaac agttttacca tattatctcg
      541 acttctggtg gtaatgctgg gttatcactg gagattcatc cgcacatgtt acgccattcg
      601 tgtggttttg ctttggcgaa tatgggaata gatacgcgac ttatctagga ttatcttggg
      661 catcgcaata ttcgtcatac tgtctggtat accgccagta atgcagggcg tttttacggc
      721 atctgggata gagccagagg gagacagcgt cacgctgttt tatagagatg gatgggatac
      781 cagggatggt gttaaaaact acattattct tacgatatat caaaaatgat taaccattta
      841 ttaattgatg ttattgtaag tatgattctt aa
//
LOCUS       DBANUU010000429          864 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.436, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000429 DBANUU010000000
VERSION     DBANUU010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.436"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            158..>864
                     /locus_tag="ACIE68_005100"
     CDS             158..>864
                     /locus_tag="ACIE68_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFX5911225.1"
                     /translation="MNSYLKISLAGITMLSCLSPTLVCAEQPHGFIDYRHEYLDDTRT
                     HADRVEFGTFFSNGIGLMGELRYNTDEGDKDKWAPSQFGNNGTGLSVVYRFKPLDDKK
                     FWLEPMFWLDTTQYWSTYEYGLSAGYDFSKEWKVSGRFRYDMDKATDKSKGYGNDDRN
                     NRRYDVWIDYRPQKTNFQYQLNLVYYNNGYLTWNDGHKNYTASFKVGYKIGSWIPYMS
                     IADYKGVDKTSSNRQIR"
BASE COUNT          286 a          162 c          167 g          249 t
ORIGIN      
        1 aaccccatca acagacgaaa ttttatctat tcattgataa ggaaaacaaa cttaaaattt
       61 aaagtcaagc tatagataca aaaacattct aatacctgga atcatcagat agtgtattcg
      121 accaccataa atatatttta ttctgaaagg gaatattatg aattcttatc ttaaaatatc
      181 acttgcagga ataacaatgt tatcctgcct gtcacctaca ctagtctgtg cagaacagcc
      241 gcatggattt attgattatc gtcatgagta tcttgatgat actcgcaccc acgcggaccg
      301 cgttgaattt ggtacatttt ttagtaatgg tatcggtttg atgggagaat tacgctataa
      361 caccgatgaa ggtgataaag ataagtgggc cccctcacaa tttggcaata acggaacagg
      421 attatctgtt gtttaccgct ttaagccgct ggatgacaaa aaattctggt tagaacccat
      481 gttctggctt gataccacgc aatattggtc aacttacgaa tatggtcttt ctgcgggtta
      541 tgacttcagc aaagaatgga aagtttcggg tcggtttcgt tatgacatgg ataaagccac
      601 cgataaatcc aaaggttatg gcaacgatga tcgtaataac cgccgttacg atgtgtggat
      661 cgactatcga ccacaaaaaa ccaacttcca atatcagtta aacctggtct attacaataa
      721 tggttattta acctggaatg atggccataa aaactatacc gcatcattta aagtagggta
      781 caaaattgga tcgtggatac cctatatgag tattgcggat tataaaggtg tggataaaac
      841 atcaagtaat cgccaaatcc gctg
//
LOCUS       DBANUU010000430          854 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.437, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000430 DBANUU010000000
VERSION     DBANUU010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.437"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            51..836
                     /locus_tag="ACIE68_005101"
     CDS             51..836
                     /locus_tag="ACIE68_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFX5911226.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          280 a          177 c          184 g          213 t
ORIGIN      
        1 atcaatgcta aatattctaa tcattatgac aggcgaggga gtgtccaatt atgaattcaa
       61 aaaagctttg ttgcatatgt gtgttattct cgctgcttgc aggatgtgcc tctgaatctt
      121 ctattgatga aaagaagaaa aaagctcaag tcacacaaag taatattaat aaaaacactc
      181 cccagcaact gacagacaaa gatttattcg gtaatgaaac cactctggcc gtatccgaag
      241 aggatattca agctgcgctt gatggagatg agttccgcgt tccccttaat tctcctgtaa
      301 tacttgttca atccggcaac cgcgcaccgg aaaccattat gcaggaggag atgcgaaaat
      361 attatactgt ttccacattc tctggtatcc cggacaggca aaagcctctg acttgtaaca
      421 aaaacaaaga taaaaatgag aacgaggatg ttgccagtgc tgagaatatg aactggatgc
      481 aggcactgcg ttttgtggct gcaaaaggac atcagaaagc gattattgtt taccaggata
      541 cattgcagac aggaaaatat gactctgcgc tgaaatcaac agtatggtcc gactataaaa
      601 atgaaaaact caccgacgct atctccctgc gctacctggt acgtttcacg ctggtggatg
      661 tggcaacagg tgagtgggct acctggtcgc cggtgaatta tgaatataaa gtgcttccac
      721 cattgcccga caagaatgaa gccagtacga ctgatatgac agagcagcaa atcatgcaac
      781 ttaagcaaaa gacctataaa gcgatggtaa aagatttggt taaccgctat caataataaa
      841 ttatatctgc cgcc
//
LOCUS       DBANUU010000431          827 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.438, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000431 DBANUU010000000
VERSION     DBANUU010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.438"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            174..249
                     /locus_tag="ACIE68_005102"
     tRNA            174..249
                     /locus_tag="ACIE68_005102"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:207..209,aa:Ile,seq:cat)
BASE COUNT          244 a          158 c          185 g          240 t
ORIGIN      
        1 aacatttgct tgttgactgc gagtatcgac tttgcgaatg gaagcggtgt cgggttttat
       61 gtcgcgtgta atgacgagca aggatgaatg agaacgcata aaaatctgtt atttacatca
      121 tatgatcatc atgtttattg cgtggtttat aagtattgtt attgcacact caaggccctt
      181 tagctcagtg gttagagcag gcgactcata atcgcttggt cgctggttca agtccagcaa
      241 gggccaccaa atcattattt acgtatcagg aagagttaag ggtacaaggg gtactcgatg
      301 gcggtctata tttcatgact aacgccattt caaggaagaa gtgcggtggg ttgtttcgaa
      361 tgagggctgg tgttttggta cgtggcacgc cactgcaatg tatttatcca tcagtactca
      421 ttcaaaagta cctggaaaaa atgcccgcta tgccaagcgg gcaaattgct tacatgtgga
      481 attaagtaat taacgataaa gccagagtat tatcatttat tatacccctt aatttaatca
      541 ggttaattac tcaaatcact gaactattct ctgttcgctc actgatgatg agtgagtacc
      601 acaaccaatg tatgtagaac aatgccgtag agtaaggatg aaatgtctct gccacgcctt
      661 aaacatctac tactgaccag aacacccgcc gcaggcagat gagttttgtg agcgttacag
      721 gagcgtggct atgctcatta accaacgcta ttgagatata aatttctaac actcgaaaaa
      781 cgaatttcac taataaatta taacgggtgg tttttatcat acagcga
//
LOCUS       DBANUU010000432          817 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.439, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000432 DBANUU010000000
VERSION     DBANUU010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.439"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..772)
                     /locus_tag="ACIE68_005103"
     CDS             complement(<1..772)
                     /locus_tag="ACIE68_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFX5911227.1"
                     /translation="MQLASRFGHVNQIRRERPLTREELMYHVPSIFGEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRREYTKHMLRLRRDGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDVMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLN"
BASE COUNT          175 a          225 c          224 g          193 t
ORIGIN      
        1 ggttgagctt aatgtcagtg tcaatgctgt gaatggcacg ggtatggata cgttttcctt
       61 tagcgctgcg acctgaaatt ccgcctttca gcatattctc ctgaatggtc tgataagcac
      121 tccacaggtc cttaccgtaa tcctcccggc gtcgtggcgt cagaatgtcg gcggtggtga
      181 cgggctgatg ttcatcacca taacggtaag tcagcgccgc ctgtgccagc gcctggcgtg
      241 ctggtggcgg caggaccagc gactgcatga catcacgctt ttcctcaatc cggtcaaaca
      301 cgcccaccac ttcgtaagca ccttcaatca ccctgtccac cacgtttccc cggtggggca
      361 cccggacttc tcccagcgac tgaccgcaga ccaggccatt ggtgcagatg gcacgaaaat
      421 atccgggtaa catctggtag ctggatgaac cgtcatggga gttgagcaga ataatttcag
      481 gcacatgctg accggttatc tgtccgtccc gccgcagacg cagcatatgt ttggtatatt
      541 cccggcggct ctggtcgcgc acacgggtct ggcaggcgaa gaacggctga aagccttccc
      601 gctgcagatt ttccaggacg gtgatggtgg gaatgtacgc ataccgttca ctgcgggagg
      661 tgtgccggtc ttctccaaaa atactcggga cgtggtacat cagttcttcg cgtgtcagtg
      721 ggcgctcccg acggatctga tttacatgac caaaacgact ggctaattgc atactttgct
      781 ccttatgaat gaattagcgg tataaacgaa aaggccg
//
LOCUS       DBANUU010000433          814 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.440, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000433 DBANUU010000000
VERSION     DBANUU010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.440"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            81..713
                     /gene="yqeH"
                     /locus_tag="ACIE68_005104"
     CDS             81..713
                     /gene="yqeH"
                     /locus_tag="ACIE68_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFX5911228.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGDKINELFIRSHSVQH"
BASE COUNT          259 a          140 c          162 g          253 t
ORIGIN      
        1 aattacaaaa tcatggtgta ttgacatcat caatcaccaa tgaaattatg caattatata
       61 cggataggga ggttcttaac atggggcttt gtagtcgtta taaaagtctt acatgcaata
      121 gttgctcaat gcattgccaa ataatgccag aagagtcacc gcgtttgcag tactgcgcaa
      181 actcgtgttt ttgtatgtgg cccgaagaaa gttcatattt taatcgtggt gtggtagaag
      241 gtatcttaac aaaaaaccac aatgcgagat taagcggata tatttttgtc gatttttcag
      301 taagtttttt acgtctattc cttgaaaagg actggattga ctatcttgca agtactgaca
      361 tggggattgt tttagtcagc gaccgcaata tgcaatcgtt agctaattac tggcgaaaac
      421 acaactcagc aatatctgct gtcatatata atgatgatgg tcttgatgtt gccaatgaaa
      481 agatcagaca actatttatt ggtcgttatt tatcatttac cagaggtaac acattaactc
      541 agatggaatt caccataatg gggtatatgg tttctggtta taatccatat cagattgctg
      601 aagttctgga tatggatatc cgtagcatct acgcgtacaa gcaacgaatc gaaaagagaa
      661 tgggtgataa aataaacgaa ttatttattc gttcacattc ggtccaacat tgatactcaa
      721 cgaccaggca gaatcatact ctgtttatac gggcatttat tgaaatgtcc gtattttata
      781 aataggccgt cccctcaaga gtaaacacca tagt
//
LOCUS       DBANUU010000434          808 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.441, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000434 DBANUU010000000
VERSION     DBANUU010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.441"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            33..>808
                     /locus_tag="ACIE68_005105"
     CDS             33..>808
                     /locus_tag="ACIE68_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFX5911229.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTDDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADC"
BASE COUNT          265 a          160 c          158 g          225 t
ORIGIN      
        1 tccatttcga ttgatttagt gtttattgac gtatgtactg gattattaac gataatatcg
       61 agttctggcc tgagcaccga aaattaatat cggtacataa cgccgatctt aacgtcgttc
      121 tgacaacgcc agccagtcga tgtttatcac ttctacttga agcttttcct gatgtggttg
      181 cacaacaaga ttttttcacc agagtctggg aagaagaagg tatgcgtgtg cctactaaca
      241 cgttatatca gaacatatcc attatcagac gcggatttcg cgctgttggt gatactaccc
      301 actcgctaat tgcaaccgtg ccgagaagag gattcaagat ccataatgac attaacatac
      361 aaaatcatgt aataaactcg tcaacagacg cacatacaca caatgcccca cctgccataa
      421 aagttaatgc gggatacaaa gagagcattg gtggcgcaaa gaatttcaat aacaaaatcc
      481 tcaaacatat aaaaagtcat ctaattatgt tgagcgcatt tgtcataggc gcatattctg
      541 catattggct atggaataat aatcaaccga agccattttt caaggattac aagactgttg
      601 cggaaattaa tggctgccat tttaatgtaa cagacgatac aattgatggg ttgaaagagt
      661 tcgataaata taaaacacgg atactggatt cggggattaa ctgtaaaaaa catccgtggt
      721 tatacttccc tcttgcaaaa tcctcaccgg ggatgattgt tatggcatgc aataaaaact
      781 ataaccaaca tgaagtggca gactgttt
//
LOCUS       DBANUU010000435          803 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.442, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000435 DBANUU010000000
VERSION     DBANUU010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.442"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..758)
                     /locus_tag="ACIE68_005106"
     CDS             complement(<1..758)
                     /locus_tag="ACIE68_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFX5911230.1"
                     /translation="MQLASRFGHVNQIRRERPLTREELMYHVPSIFGEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRREYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRAIHSIDT"
BASE COUNT          168 a          229 c          219 g          187 t
ORIGIN      
        1 tcggtgtcga tgctgtgaat ggcacgggta tggatacgtt ttcctctggc actgcgaccg
       61 gaaattccgc ctttcagcat attctcctga atggtctgat aagcactcca caggtcctta
      121 ccgtaatcct cccggcgtcg tggtgtcaga atgtcggcgg tggtgacggg ctgatgttcg
      181 tcaccataac ggtaagtcag tgccgcctgt gccagcgcct ggcgtgccgg tggcggcagg
      241 accagcgact gcatggcatc acgcttctcc tctatccggt caaacacgcc caccacttcg
      301 taagcccctt caatgactct gtccaccaca tttccccggt gcggaacacg cacttccccc
      361 agagactggc cacagacgca cccgttctgg cagacgaacc tgaagtaacc cggcagcatc
      421 tggtagctgg aggtaccgtc atgagagttg agcagaataa tttcagggac atgttctccg
      481 tttatctctc cggcccgccg cagacgcagc atgtgtttgg tatattcccg gcggctctgg
      541 tcgcgcacac gggtctggca ggcgaagaac ggctgaaagc cttcccgctg cagattttcc
      601 aggacggtaa tggtgggaat gtacgcatac cgttcactgc gggaggtgtg ccggtcttct
      661 ccaaaaatac tcgggacgtg gtacatcagt tcttcgcgtg tcagtgggcg ctcccgacgg
      721 atctgattta catgaccaaa acgactggct aattgcatac tttgctcctt atgaatgaat
      781 tagcggtata aacgaaaaag ccg
//
LOCUS       DBANUU010000436          794 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.443, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000436 DBANUU010000000
VERSION     DBANUU010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.443"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
BASE COUNT          286 a          184 c          117 g          207 t
ORIGIN      
        1 cttcaaatac ttctgtataa tcgcagccag ccaatgatga gaatggataa aaacaacact
       61 tcctcgacaa acacaaaaac cagccttcat tttcacatca tcatccagtt ttaattaaac
      121 cctgacgagc gacagtattt cagttcagga ataaaaagcc agctattact cagtaaaaac
      181 accaccaact aataatatct cagcagatat ttcctgacca gggtgacaca tttattaaag
      241 atttttaaca ggacacttta cattactgaa cgctattccg acagtatcac tacacaaata
      301 aaaaagggaa ctggataaat attcatgccg gaagaatgat gtaacaaaag catttaatca
      361 ttacgtgcgt attcctctct attcgatcac ggtttggcca aaccgtgatc gattcagatg
      421 ccattttgca agtacgcatg acccggaagg gaaacactta acaacatggt atcaacagat
      481 atcattacgt gcgcactctt ataaaaatac acagcagaag caagataact ctaaacgtaa
      541 atttaatatc tcccactgac tgccccgaca atatactgaa aaatactcac ctcagtaaac
      601 ataaacacac ctgataatca ggctgcaaga tgaatatttt tctcctttac ggagtaatca
      661 gcctgctgtt taacaataac cgcgtatttt catggaagga gatcacccag taacggataa
      721 gtcctgatct ccttcatgtt aatacgcaac aatcccatcg attcaaaaca atttcagaca
      781 gatattatct ccgt
//
LOCUS       DBANUU010000437          788 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.444, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000437 DBANUU010000000
VERSION     DBANUU010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.444"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..486)
                     /gene="sctT"
                     /locus_tag="ACIE68_005107"
     CDS             complement(<1..486)
                     /gene="sctT"
                     /locus_tag="ACIE68_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFX5911231.1"
                     /translation="MEEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALFQHYHYELSTLNEIDIALFAAREIIIGLFIACLLSSPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTNGGLNFILETLWQSYNLWPSG
                     NF"
     gene            complement(488..748)
                     /locus_tag="ACIE68_005108"
     CDS             complement(488..748)
                     /locus_tag="ACIE68_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFX5911232.1"
                     /translation="MNDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
BASE COUNT          279 a          168 c          137 g          204 t
ORIGIN      
        1 aaaattgcct gaaggccata aattataact ttgccaaaga gtttccagaa taaagttcaa
       61 tccaccgtta gtaagataaa ccgctgcaga gaataggtta aataatcttg ctaattccga
      121 agtatcgaca ccagtagcag gatccaaagt actacttaac gttgcaccac gttggttatc
      181 gataaaacta cctatagcaa ggaaaatcca aaacggggat gataataagc aagcaatgaa
      241 cagccctatg ataatttctc tggcagcaaa caaagcgata tcgatttcat ttaaggtcga
      301 taattcatag tggtaatgct gaaataatgc acatgatacc actattatta ttggatgacg
      361 gactactgtt ggtatattcc cactggcgaa aaatggtaac aaatagaaag ttggtgccaa
      421 tcggcaaaaa ccgagcgctg tagcagccag cagactatgc aactgataga gtatcgcctc
      481 ctccatccta tcctctcgcg aatgctaaat tcagcatttc gataccaaac gaatataatt
      541 tttcaccata ccatccgctc attaaaaaaa agcaaatact tacgcataat aatttgacgc
      601 caaatggcaa cgtctgttct tgtaactgtg ttaccgtttg aaaaagccct accaataaac
      661 caacaaatgt cgcaactgcg ataggtcctg cagacataac taaaatcaga tacagcgccc
      721 tattccctgc aaaaacaata tcgttcatat tttttcctca gggattaatg gataggtcaa
      781 aatattgt
//
LOCUS       DBANUU010000438          781 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.445, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000438 DBANUU010000000
VERSION     DBANUU010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.445"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(32..433)
                     /locus_tag="ACIE68_005109"
     CDS             complement(32..433)
                     /locus_tag="ACIE68_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFX5911233.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(693..>781)
                     /locus_tag="ACIE68_005110"
     CDS             complement(693..>781)
                     /locus_tag="ACIE68_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFX5911234.1"
                     /translation="VLERADYLAKEYTKAHCTGERNFGCSRS"
BASE COUNT          173 a          196 c          215 g          197 t
ORIGIN      
        1 tggcctttgc tgtcattcgc catataacag ttcagaattc gcatatttcc tcctgagttg
       61 tccggggaat actgaatcct ccggtctggc agtgtgtgca gtactgctcc ccataataat
      121 cgtggtgaca ttgtctgcag tgccagctgg ctttacgcac cacgggtaag gcatccggta
      181 cgaattgctg cagacgttta atcaactgta tttctctgcg ctccggcctg acataagggc
      241 actgttgacc gtgctctgtc agcctgtcgt cagtgtgttc aaaccaggga agttcagtgt
      301 cgtattgcgg atggtatctg agtgcactgc cacacaagtg gcaggtgtag tgagcataag
      361 gtgcggcctg tgcagtacgg gcgcccgtca gacgtccgtt gccatcaaga gcgataaagg
      421 attttgcgta catagtcata ttccttagcc gccagacgac acgcaggcaa caaccgtccc
      481 ggtacgggca gcgtggtcag ggtgtgaatg gcggttcagt taaaatctgg actggagttt
      541 gggtaaagca ggctggttgc tgttacagca ctgtggttgt cacacaatgg aacactgacg
      601 taaaaaacag tcagtgggcg agtggataca tcgttgccgc cagacaacaa cccatacttt
      661 tggtttgccg gatattggcg cgccagtcgt gctcaactcc ggctacagcc aaaattgcgc
      721 tcaccggtgc agtgagcttt ggtatactcc ttcgccagat agtcagcacg ttccagcact
      781 t
//
LOCUS       DBANUU010000439          776 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.446, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000439 DBANUU010000000
VERSION     DBANUU010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.446"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>776
                     /locus_tag="ACIE68_005111"
     CDS             <1..>776
                     /locus_tag="ACIE68_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFX5911235.1"
                     /translation="APEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGA
                     AQMDGAILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEV
                     RELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFL
                     LPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGR
                     AGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVY"
BASE COUNT          186 a          196 c          220 g          174 t
ORIGIN      
        1 gcgccggaag aaaaagctcg tggtatcacc atcaacactt ctcacgttga atacgacacc
       61 ccgacccgtc actacgcaca cgtagactgc ccggggcacg ccgactatgt taaaaacatg
      121 atcaccggtg ctgctcagat ggacggcgcg atcctggtag ttgctgcgac tgacggcccg
      181 atgccgcaga ctcgtgagca catcctgctg ggtcgtcagg taggcgttcc gtacatcatc
      241 gtgttcctga acaaatgcga catggttgat gacgaagagc tgctggaact ggttgaaatg
      301 gaagttcgtg aacttctgtc tcagtacgac ttcccgggcg acgacactcc gatcgttcgt
      361 ggttctgctc tgaaagcgct ggaaggcgac gcagagtggg aagcgaaaat cctggaactg
      421 gctggcttcc tggattctta cattccggaa ccagagcgtg cgattgacaa gccgttcctg
      481 ctgccgatcg aagacgtatt ctccatctcc ggtcgtggta ccgttgttac cggtcgtgta
      541 gaacgcggta tcatcaaagt tggtgaagaa gttgaaatcg ttggtatcaa agagactcag
      601 aagtctacct gtactggcgt tgaaatgttc cgcaaactgc tggacgaagg ccgtgctggt
      661 gagaacgtag gtgttctgct gcgtggtatc aaacgtgaag aaatcgaacg tggtcaggta
      721 ctggctaagc cgggcaccat caagccgcac accaagttcg aatctgaagt gtacat
//
LOCUS       DBANUU010000440          771 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.447, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000440 DBANUU010000000
VERSION     DBANUU010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.447"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            32..748
                     /locus_tag="ACIE68_005112"
     CDS             32..748
                     /locus_tag="ACIE68_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFX5911236.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          255 a          133 c          167 g          216 t
ORIGIN      
        1 atgtaaataa attttcgagg agatcattcc agtgggacgt aaatgggcca atattgttgc
       61 taaaaaaacg gctaaagacg gtgcaacgtc taaaatttat gcaaaattcg gtgtagaaat
      121 ctatgctgct gctaaacaag gtgaacccga tccagaatta aacacatctt taaaattcgt
      181 tattgaacgt gcaaagcagg cacaagttcc aaagcacgtt attgataaag caattgataa
      241 agccaaaggc ggcggagatg aaacgttcgt gcagggacgt tatgaaggct ttggtcctaa
      301 tggctcaatg attatcgccg agacattgac ttcaaatgtt aaccgtacga ttgctaacgt
      361 tcgcacaatt ttcaataaaa aaggcggcaa tatcggagcg gcaggttctg tcagctatat
      421 gtttgacaat acgggtgtga ttgtatttaa agggacagac cctgaccata tttttgaaat
      481 tttacttgaa gctgaagttg atgttcgtga tgtgactgaa gaagaaggta acattgttat
      541 ttatactgaa cctactgacc ttcataaagg aatcgcggct ctaaaagcag ctggaatcac
      601 tgagttctca acaacagaat tagaaatgat tgctcaatct gaagttgagc tttccccaga
      661 agatttagaa atctttgaag ggcttgttga tgcccttgaa gatgacgacg atgtacaaaa
      721 agtttatcat aacgtcgcaa atctctaatt atcttttaaa gaaatctgtc t
//
LOCUS       DBANUU010000441          770 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.448, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000441 DBANUU010000000
VERSION     DBANUU010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.448"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..727
                     /gene="istB"
                     /locus_tag="ACIE68_005113"
     CDS             <1..727
                     /gene="istB"
                     /locus_tag="ACIE68_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFX5911237.1"
                     /translation="ESLISAAPALSQQAVDQEWSYMDFLEHLLHEEKLARHQRKQAMY
                     TRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERNENIVLLGPSGVGKTHLAI
                     AMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPF
                     SQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLDRILHHSHVVQ
                     IKGESYRLRQKRKAGVIAEANPE"
BASE COUNT          222 a          187 c          197 g          164 t
ORIGIN      
        1 ggaaagcctt ataagcgcag cgcctgcgct gtcacaacag gcagtagacc aggaatggag
       61 ttatatggac ttcctggagc atctgcttca tgaagaaaaa ctggcacgtc atcaacgtaa
      121 acaggcgatg tatacccgaa tggcagcctt cccggcggtg aaaacgttcg aagagtatga
      181 cttcacattc gccaccggag caccgcagaa gcaactccag tcgttacgct cactcagctt
      241 catagaacgt aatgaaaata tcgtattact ggggccatca ggtgtgggga aaacccatct
      301 ggcaatagcg atgggctatg aagcagtccg tgcaggtatc aaagttcgct tcacaacagc
      361 agcagatctg ttacttcagt tatctacggc acaacgtcag ggccgttata aaacgacgct
      421 tcagcgtgga gtaatggccc cccgcctgct catcattgat gaaataggct atctgccgtt
      481 cagtcaggaa gaagcaaagc tgttcttcca ggtcatcgct aaacgttacg aaaagagcgc
      541 aatgatcctg acatccaatc tgccgttcgg gcagtgggat caaacgttcg ccggtgatgc
      601 agcactgacc tcagcgatgc tggaccgtat cttacaccac tcacatgtcg ttcaaatcaa
      661 aggagaaagc tatcgactca gacagaaacg aaaggccggg gttatagcag aagctaatcc
      721 tgagtaaaac ggtggatcaa tattgggccg ttggtggaga tataagtgga
//
LOCUS       DBANUU010000442          768 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.449, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000442 DBANUU010000000
VERSION     DBANUU010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.449"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            10..>768
                     /locus_tag="ACIE68_005114"
     CDS             10..>768
                     /locus_tag="ACIE68_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFX5911238.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYN"
BASE COUNT          240 a          170 c          141 g          217 t
ORIGIN      
        1 gagtagtgta tggccaatct ctacgacttg aaaaagttcg acttaaacct tcttgtcata
       61 tttgagtgta tttaccagca tttaagcatt agcaaagctg ctgaatcact atatatcacc
      121 ccatcagccg tgagccagtc gttacaacgc ttacgtgcgc aatttaatga ccctttattc
      181 attcgctcag gaaaaggtat cgcgccgacg acaacagggt tgaatctgca tcaccatctt
      241 gagaaaaacc tcaaaggcct tgaacaaaca attaatatag tcaataagtc cgaactaaag
      301 aaaaacttta ttatctacgg ccctcagctc atttcttgct ctaataatag tatgctgatc
      361 cgctgcctac ggcaagatgc ctctgtcgag attgagtgcc atgatatcct catgtcagca
      421 gagaatgctg aagaactgtt ggttcaccgc aaagccgatc tggtgatcac ccaaatgcca
      481 gtgatcagcc gttccgtcat ttgcatgcca ttgcatacaa ttcgcaatac tctaatttgt
      541 agtaataagc atccgcgaat tactgacaat agcacttatg aacaaattat ggcagaagag
      601 ttcacccaat taatatccaa atcagcagga gttgatgata ttcaaatgga aatagacgag
      661 agatttatga atcgcaaaat ctcatttcgt ggttcctctt tgctgacaat cattaatagc
      721 attgcagtta cagatttact gggaatagtc ccctatgaac tttataat
//
LOCUS       DBANUU010000443          767 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.450, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000443 DBANUU010000000
VERSION     DBANUU010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.450"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>767)
                     /locus_tag="ACIE68_005115"
     CDS             complement(<1..>767)
                     /locus_tag="ACIE68_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFX5911239.1"
                     /translation="PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGY
                     YVSEDGYPGWMPQKWTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLD
                     TLQNTQLQSAIVKAMYAATIESELDTQSAMDFILGANSQEQRDKLTGWIGEIAAYYAA
                     APVRLGGAKVPHLMPGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYA
                     QMSYSTARASANESWAYFMGRRKFVASRQASQMFLCWL"
BASE COUNT          153 a          249 c          204 g          161 t
ORIGIN      
        1 agccagcaca gaaacatctg gctcgcctga cgggatgcga cgaattttcg ccgccccata
       61 aaatacgccc acgactcgtt cgcactggcc cgtgccgtgg agtagctcat ctgggcgtaa
      121 ttccgggaaa gctgctcata cgagacaccc agcccggcag cgatataccg cagcagtgac
      181 tgctcaaaca cggagtagcc gttatccgta tcctgagccg tctgcaggtt cagtgagtca
      241 cccggcatca ggtgcggcac ttttgcgcct ccaagccgga ccggcgctgc ggcgtaatac
      301 gcggcaattt caccaatcca gccggtcagc ttgtcccgct gctcctgact gttcgcgccc
      361 agaataaaat ccatcgctga ctgcgtatcc agctcactct caatggtggc ggcatacatc
      421 gccttcacaa tggcgctctg cagctgcgtg ttctgcagcg tgtcgagcat cttcatctgc
      481 tccattacgc tgtaaaacac atttgcaccg cgggtctgcc cgtcctccac gggttcaaaa
      541 acgtgaatga acgaggcgcg cccgccgggt aactcacggg gtatccatgt ccatttctgc
      601 ggcatccagc caggatagcc gtcctcgctg acgtaatatc ccagcgccgc gccgctgtca
      661 ttaatctgca caccggcacg gcagttccgg ctgtcgccgg tattgttcgg gttgctgata
      721 cgcttcggac tgaccatccg gaactgtgtc cggaacagtc gcgacgg
//
LOCUS       DBANUU010000444          756 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.451, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000444 DBANUU010000000
VERSION     DBANUU010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.451"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            1..273
                     /gene="yddL"
                     /locus_tag="ACIE68_005116"
                     /pseudo
     CDS             1..273
                     /gene="yddL"
                     /locus_tag="ACIE68_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            312..>756
                     /locus_tag="ACIE68_005117"
     CDS             312..>756
                     /locus_tag="ACIE68_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFX5911240.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKYLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQ"
BASE COUNT          267 a          154 c          128 g          207 t
ORIGIN      
        1 aaattaaaaa tagttgcggt ggttgtaact ggtttgttag ctgcgaacgt agcacacgct
       61 gccgaagtct ataacaagga tggtaataaa ctcgaccttt atggcaaggt taccgctcta
      121 cgttatttta ctgatgataa gcgtgacgat ggtgataaaa cttatgcccg tctcggcttt
      181 aaaggagaaa cgcaaatcaa tgatcaaatg attggttttg gtcactggga atatgatttt
      241 aaaggctata acgatgaagc caactgctcg cgcggcaaga atctctgatt gagttaagtg
      301 cccatcacaa tatgatcact gaccttatat tacacaatca tcccaggatg aaaacaatca
      361 ctttaaacga caaccatatt gcacatttaa acgccaaaaa cactacaaaa ctggaatatt
      421 taaacttaag caataacaat ttactgccaa ccaatgacat tgatcaacta atatcatcaa
      481 aatatctttg gcatgtatta gttaacggca tcaacaatga tccacttgca caaatgcagt
      541 actggactgc agtaagaaat ataattgatg acactaatga agtgaccatt gatttatcag
      601 gacttaattt aaccactcaa ccaccagggc tgcaaaactt cacctctatc aatcttgata
      661 ataaccaact cacacatttt gatgcaacca actacgatag actcgtaaag ctaagtctga
      721 atagtaatgc tcttgagtca ataaattttc ctcaag
//
LOCUS       DBANUU010000445          751 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.452, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000445 DBANUU010000000
VERSION     DBANUU010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.452"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            157..723
                     /gene="slp"
                     /locus_tag="ACIE68_005118"
     CDS             157..723
                     /gene="slp"
                     /locus_tag="ACIE68_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFX5911241.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          229 a          161 c          164 g          197 t
ORIGIN      
        1 atgccacgcc ccaaaatcaa tggaattgaa ctaaacaaaa gggatataaa catcagacag
       61 gtttacgtta ctatcaggca tatcacctca gaatcagatg aaaactataa agaaatatct
      121 attatggctt taatatttgt tgataaggat agtaacatga acatgacaaa aggtgcactc
      181 atcctcagcc tttcattttt gcttgccgca tgtagttcaa ttccgcaaaa tatcaaaggc
      241 aataaccaac ctgatattca aaaaagtttt gttgctgttc ataaccagcc ggggttatat
      301 gttggtcaac aagcgcgctt tggtgggaag gttatcaacg ttatcaatgg caaaacggat
      361 acgttgttag aaatcgctgt attaccgttg gatagctatg cgaagcctga tattgaagcc
      421 aactatcagg gccgactgct cgccagacaa agcggcttcc ttgatccagt gaactatcgt
      481 aatcactttg ttaccatcct cggtaccatt cagggtgaac aaccaggctt tatcaataaa
      541 gtcccgtata acttcctgga agtgaatatg cagggcatcc aggtgtggca tttgagagaa
      601 gtggttaata ccacctataa cctgtgggat tacggctatg gtgcattctg gccggaaccg
      661 ggctggggtg cgccttacta caccaatgcg gtgagtcagg taacacctga gctggtcaaa
      721 taacaccacc gaaagatgca gatgctttct c
//
LOCUS       DBANUU010000446          750 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.453, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000446 DBANUU010000000
VERSION     DBANUU010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.453"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..355)
                     /gene="traA"
                     /locus_tag="ACIE68_005119"
     CDS             complement(<1..355)
                     /gene="traA"
                     /locus_tag="ACIE68_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFX5911242.1"
                     /translation="MNTVLSVQGASAPVKKKSFFSKFTRLNMLRLVRAVLPAAVLMMF
                     FPQLAMASGSQDLMASGNSTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFLAG
                     FAIISVFIAVGMAVVG"
     gene            complement(388..615)
                     /gene="traY"
                     /locus_tag="ACIE68_005120"
     CDS             complement(388..615)
                     /gene="traY"
                     /locus_tag="ACIE68_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFX5911243.1"
                     /translation="MSRNIIRPAPGNKVLLVLDDATNHKLLGARERSGRTKTNEVLVR
                     LRDHLNRFPDFYNLDAIKEGAEETDSIIKDL"
BASE COUNT          247 a          180 c          136 g          187 t
ORIGIN      
        1 ggccaacgac ggccataccc acagcaataa atacagagat gatggcaaaa ccggccagga
       61 acttgacgtt tttggtcatc atgtacatga cagcaccgac cagaacttca gccagaacaa
      121 cccatttaac aacactggag tccttaccga aggtcgcctt aaccgtggag ttaccacttg
      181 ccatcaggtc ctgactaccg gaagccatcg ccagttgcgg gaagaacatc atcagaacag
      241 cagccgggag cacagcgcgt accaggcgaa gcatattcag acgagtgaat ttagaaaaaa
      301 acgatttctt tttgacgggc gcagaagcac cctgaacact taaaacagta ttcataagtt
      361 acctctattg tggatagatt aataaaccta aagatcttta attatcgaat cagtttcctc
      421 tgctccctcc tttattgcat ccagattata aaagtcagga aaacggttca gatgatcacg
      481 taacctgact agcacctcat tagtttttgt tctcccacta cgttcccggg cacctaagag
      541 cttgtgatta gtagcatcgt ccaataccag taaaacttta ttgcccggag caggccttat
      601 tatatttctg ctcaagtctc ctcctcagcc acttagataa ttaaatataa ttatacgaat
      661 ccataaaaat agaaacaaaa ataacgtaaa ttatattaac aaaaaaggga accgatggtt
      721 cccttaagtt cacaataaat taacacttgt
//
LOCUS       DBANUU010000447          740 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.454, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000447 DBANUU010000000
VERSION     DBANUU010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.454"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            10..>740
                     /locus_tag="ACIE68_005121"
     CDS             10..>740
                     /locus_tag="ACIE68_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFX5911244.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRTYLQK
                     SVPMTITVLGTPLSWNAKYSADASFT"
BASE COUNT          202 a          178 c          211 g          149 t
ORIGIN      
        1 ttccgggaaa tgaaacgtat tttacctctg atactggctc tggttgccgg catggcacag
       61 gctgacagca acagtgatta ccgggccggc tctgattttg ctcaccagat caaaggacag
      121 ggaagcagca gtattcaggg ctttaagcca caggagagta tccccggcta taacgcgaat
      181 ccggacgaga caaaatatta cggtggcgtg actgccggcg gggatggcgg cctgaaaaat
      241 gacggcacca cggaatgggc gaccggtgaa accggaaaaa ccatcacaga gtcattcatg
      301 aacaagccga aagacattct ttcaccggat gcaccgttta tccagaccgg cagggatgtg
      361 gtgaaccggg ctgacagcat tgtgggaaac accgggcagc agtgcagtgc gcaggagatt
      421 agccggagtg aatacacgaa ttacacctgt gagcgggatt tacaggtgga gcagtactgc
      481 acacgaactg cccgtatgga gctgcagggg agtaccacat gggaaacccg gacgctggag
      541 tatgaaatga gtcagctacc tgcacgtgaa gtgaatggtc agtatgttgt ctccattacc
      601 tctcccgtta ccggtgaaat tgtcgatgcg cattacagct ggagtcgtac ttacctgcag
      661 aagagtgtac ctatgacaat tacagtactg ggaaccccac tgagctggaa tgccaaatac
      721 tcagcagatg cctcatttac
//
LOCUS       DBANUU010000448          731 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.455, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000448 DBANUU010000000
VERSION     DBANUU010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.455"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>731)
                     /locus_tag="ACIE68_005122"
     CDS             complement(<1..>731)
                     /locus_tag="ACIE68_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFX5911245.1"
                     /translation="GNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDI
                     VWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAIN
                     FSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEG
                     IPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMD
                     FAELLLEDYKASLKYLSDHPKLQGIA"
BASE COUNT          170 a          206 c          206 g          149 t
ORIGIN      
        1 gcaataccct gcagtttcgg gtgatcgctg agatatttca gggaggcttt gtagtcttcc
       61 agcaacagtt cagcaaagtc catttcgaag ccgcgacgac acataatgcg catcaccacg
      121 atgtcggtgg cttcaccgcc gagagtgaag gccggaacct gccagccgcg cagacgcaga
      181 cgttcagaga ggtcgtacag ggtgtatccc ggatcttcac catctttcag tttgaagcaa
      241 accgccggga tgccttcgtc cgggcgaccc gtacagatga actcatacgg ccccagtttg
      301 gcgatttcat ccgccagata agcggcaacc tggtaagagg cgttctgtac tttggtatag
      361 ccttcacgac cgaggcgcag gaattcatag tactgtgcaa ttacctgacc cgccgggcgg
      421 gagaagttga tggcaaaagt accaatttga ccacccaggt agtcaacgtt gaacaccagt
      481 tcctgcggca gcgcttcttc gtcacgccag ataacccagc cgcagcccag cggagccaga
      541 ccgaatttat ggcctgaagc actgatcgat ttcacacgcg gcaggcggaa gtcccagacg
      601 atatccgggg cgacgaacgg tgccaggaag ccaccgctgg cagcgtcgat gtgcatgtcg
      661 atgtcgatac cggtgtcggc ctggaattta tccagcgcat cgtgcagcgg ttgtgggaac
      721 tcatagttac c
//
LOCUS       DBANUU010000449          727 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.456, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000449 DBANUU010000000
VERSION     DBANUU010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.456"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(160..>727)
                     /gene="lpfA-O113"
                     /locus_tag="ACIE68_005123"
     CDS             complement(160..>727)
                     /gene="lpfA-O113"
                     /locus_tag="ACIE68_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFX5911246.1"
                     /translation="RNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSDTA
                     DQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDLKV
                     GTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEMKF
                     IARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          203 a          176 c          156 g          192 t
ORIGIN      
        1 ctgcataagt taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact
       61 tacatgaaac tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc
      121 cctggccaaa gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt
      181 aaattgtgaa tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg
      241 tgcaataaac ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt
      301 ataaagttta acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc
      361 cgcaatcgct tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc
      421 cactttcagg tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg
      481 agtgtacgtt gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact
      541 acctacacct gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt
      601 atcggaggta acttcacaag tagtgtcgac aatttcaccg acgaagtgaa caacaccatc
      661 ttctgccagt gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat
      721 attacgc
//
LOCUS       DBANUU010000450          726 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.457, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000450 DBANUU010000000
VERSION     DBANUU010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.457"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(28..>726)
                     /locus_tag="ACIE68_005124"
     CDS             complement(28..>726)
                     /locus_tag="ACIE68_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFX5911247.1"
                     /translation="LCGCKQQELLNHLDQQQANDVLAVLQRHNINAEKKDQGKTGFSI
                     FVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKARLYSAIEQRLEQS
                     LKIMDGIISSRVHVSYDVDNGDSGKTALPIHISVLAVYEKDINPEIKINDIKRFIVNS
                     SASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLALISVATCWLLWK
                     YRAILTNLVRLKIK"
BASE COUNT          222 a          146 c          123 g          235 t
ORIGIN      
        1 ggcaagattc ataacgctct catatgtcta cttaattttc aacctgacta aattagttag
       61 aatggcccta tacttccata acagccagca agtcgctacg gatattaatg caagtaagat
      121 agaaaccggc atagccttat cataagcaaa aacaggttcg ctaatttcat atgttggtgc
      181 ttgctcaata atgtctcttc gttttgacaa tacaacagaa atattttcat attgtacgct
      241 tgcagagcta ttaacaataa atctcttgat atcatttatt tttatttctg ggttgatatc
      301 tttttcatat actgcaagta cagaaatatg aattggtaaa gcagttttac cactatcgcc
      361 attatcaaca tcgtaactaa catgtactct cgaagaaata atgccatcca taattttgag
      421 agattgctct aaccgctgct caatagcaga atatagcctt gctttttccg ctcgtggaga
      481 tgaaacgagt gcatctgcag ggaacatctg cgatatttga atatcaggct tacccggtag
      541 attgtagatt tttagccaat ccaccgcaga agcaaaatcc gttggttcga caaatattga
      601 aaatcctgtt ttgccttgat ccttcttttc agcattaata ttatgtcttt gtaaaacagc
      661 aaggacatca ttagcttgct gttgatcaag atggttcaat aattcctgct gcttgcagcc
      721 gcacaa
//
LOCUS       DBANUU010000451          725 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.458, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000451 DBANUU010000000
VERSION     DBANUU010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.458"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(24..212)
                     /gene="bcsR"
                     /locus_tag="ACIE68_005125"
     CDS             complement(24..212)
                     /gene="bcsR"
                     /locus_tag="ACIE68_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFX5911248.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            485..>725
                     /locus_tag="ACIE68_005126"
     CDS             485..>725
                     /locus_tag="ACIE68_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BcsE family c-di-GMP-binding protein"
                     /protein_id="HFX5911249.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKI"
BASE COUNT          205 a          160 c          156 g          204 t
ORIGIN      
        1 cagtacggcc attcaccaat cccctacttt tgttgcgcaa actctgccag caacggccag
       61 cgttttaatg ccgcggccaa ctgttcgcgt tgggaaatat cggcataatc aatatcaggc
      121 agtgaaaatg cctgctttaa cgccacaata tcattctgga agatatagcc tatcgcggga
      181 tcaggcagag tatctggttc attgttattc attttgcttg atccctatga aataaccgta
      241 ccgttcatga gacaattcta accgtgagca tttcgccggt ttattgttta cggcgagacg
      301 ggaatttgct cactttttga ctttccgtta cgcaacagaa aaagttaaaa acgaaaagac
      361 taaaatcaca cgtcttgtga ccttctaaat tgattcacat gctaaatctg ataagtttta
      421 atttcaatgg taggtttatt tcttagcttt cgctagtaaa ctgataatca gacaaaatgg
      481 tgacatgagg gacattgtgg accctgtatt ctctatcggt atctcatcat tatgggatga
      541 gctgcgacat atgccagcag gcggcgtctg gtggtttaac gtcgatcgcc atgaagatgc
      601 tatcagtctg gcgaatcaaa caattgcatc ccaggctgaa accgcacacg tcgcggtcat
      661 tagcatggac agcgatccgg cgaaaatctt tcaattagat gattctcaag ggccggaaaa
      721 aataa
//
LOCUS       DBANUU010000452          719 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.459, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000452 DBANUU010000000
VERSION     DBANUU010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.459"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>719
                     /locus_tag="ACIE68_005127"
     CDS             <1..>719
                     /locus_tag="ACIE68_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFX5911250.1"
                     /translation="DLPTSQFWSYATYFRVLSFEYLSESISTLLYLDADVVCKGSLKP
                     LTEIIFKDEFAAVIPDNDSTQEACAKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPY
                     LLKLLRGETKYGSLKYLDQDALNIAFNMNNIYLGKDFDTIYTLKNELHDRSHRKFQQT
                     ITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQKQYKH
                     LFAHGEYIKGITSLIKYKLKK"
BASE COUNT          232 a          134 c          143 g          210 t
ORIGIN      
        1 ctgatctgcc gacgtcacag ttttggtcgt acgcgacata cttcagggta ttgtcttttg
       61 aatatctgag tgaaagtatt tccacactgc tgtatctgga tgccgatgtt gtttgtaaag
      121 gaagcctgaa acctctcaca gaaattatat ttaaagatga gtttgctgcg gtcattcctg
      181 acaatgatag tacgcaggag gcatgtgcaa aacgcctcaa cattcccgaa atgaatggac
      241 gttatttcaa tgcaggcgtt atctatgtca atcttaaaaa atggcatgaa gcaaatttga
      301 caccgtattt actcaagctt ttacgagggg aaactaaata tggctctctt aaatatttag
      361 atcaggatgc gttgaatatc gcatttaata tgaataatat ctacctcggg aaggattttg
      421 atactattta taccctgaaa aatgaacttc atgatcgtag tcatcgaaag tttcagcaaa
      481 ccattaccga taaaacagta ttgattcact atacagggat aactaaacca tggcatagct
      541 gggctggata tccgtctgca tcatacttta atatcgcgcg tgaacaatct ccctggaaga
      601 aatatcctct taaagaggcg cggactgttg cagaaatgca gaaacaatat aagcatctgt
      661 ttgcccatgg tgagtatata aaaggcataa cttcattaat taagtacaag cttaagaaa
//
LOCUS       DBANUU010000453          719 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.460, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000453 DBANUU010000000
VERSION     DBANUU010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.460"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>719)
                     /locus_tag="ACIE68_005128"
     CDS             complement(<1..>719)
                     /locus_tag="ACIE68_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HFX5911251.1"
                     /translation="ALLTVTPAASADVNSDMNQFFNKLGFASNTTQPGVWQGQAAGYA
                     YGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQLQRFVKQIMSNAA
                     GYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGGLFPRTQVSQQKV
                     CQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNINIMWNALSKNRM
                     FDGNKELKEFVMTLTGSLVFGP"
BASE COUNT          161 a          202 c          180 g          176 t
ORIGIN      
        1 ggaccaaaca ccagtgagcc ggtcagcgtc atcacaaact ctttcagctc tttgttgccg
       61 tcaaacattc tgtttttgga aagcgcattc cacatgatgt tgatgttttt ggtcacccgc
      121 tccttatcct tgtcgctggc tttatccctg acactgtcag atttcccgcc aacggtacat
      181 ccctgccggg aagccgccca gtcagcaaaa atattgctct caccggcaat gtcctgacag
      241 actttctgct gggacacctg cgtccggggg aagagcccgc cgataatccc ctgtgccgcc
      301 tggcaggaac tgaggttcat actgttaatg tcgcttgcca ttttctgcag gaagtctttt
      361 gcggttttga tttccggcac cgttgtctgc agggcaaggt caaaaaagta accggcagca
      421 ttgctcataa tctgcttaac aaaacgctgc agctgctcgc cattaataaa actgaacgag
      481 ccgagatagg cgtcgatgcc gccacatccg gcattgatat ccggcagcgt catggaaatc
      541 agctgaacat ttttgacctg agtacgggca tacagggagc caccgtaagc ataaccggcg
      601 gcctgcccct gccagacgcc cggctgtgtg gtattggagg cgaagcccag cttgttaaag
      661 aactgattca tgtcgctgtt cacatccgct gacgctgcgg gtgtgaccgt cagtaaagc
//
LOCUS       DBANUU010000454          719 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.461, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000454 DBANUU010000000
VERSION     DBANUU010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.461"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>719)
                     /locus_tag="ACIE68_005129"
     CDS             complement(<1..>719)
                     /locus_tag="ACIE68_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HFX5911252.1"
                     /translation="ALLTVTPAASADVNSDMNQFFNKLGFASNTTQPGVWQGQAAGYA
                     YGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQLQRFVKQIMSNAA
                     GYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGGLFPRTQVSQQKV
                     CQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNINIMWNALSKNRM
                     FDGNKELKEFVMTLTGSLVFGP"
BASE COUNT          164 a          202 c          176 g          177 t
ORIGIN      
        1 ggaccaaaca ccagtgagcc ggtcagcgtc atcacaaact ctttcagctc tttgttgccg
       61 tcaaacattc tgtttttgga aagcgcattc cacatgatgt tgatgttttt ggtcacccgc
      121 tccttatcct tgtcgctggc tttatccctg acgctgtcag atttcccgcc aacggtacat
      181 ccctgccggg aagccgccca gtcagcaaaa atattgctct caccggcaat gtcctgacag
      241 actttttgct gggacacctg cgtccggggg aaaagcccgc cgataatccc ctgtgcagcc
      301 tggcaggaac tgaggttcat actgttaatg tcgcttgcca ttttctgcag gaagtctttt
      361 gcggttttta tttccggcac cgttgtctgc agggcaaggt caaaaaagta accggcagca
      421 ttactcataa tctgcttaac aaaacgctgc agctgctcgc cattaataaa actgaacgaa
      481 ccgagataag cgtcgatgcc gccacatccg gcattgatat ccggcagcgt catggaaatc
      541 agctgaacat ttttgacctg agtacgggca tacagggagc cgccgtaagc ataaccggcg
      601 gcctgcccct gccagacgcc cggctgtgtg gtattggagg cgaagcccag cttgttaaag
      661 aactgattca tgtcgctgtt cacatccgct gacgctgcag gcgtcaccgt cagtaaggc
//
LOCUS       DBANUU010000455          708 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.462, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000455 DBANUU010000000
VERSION     DBANUU010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.462"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            95..184
                     /locus_tag="ACIE68_005130"
                     /pseudo
     CDS             95..184
                     /locus_tag="ACIE68_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          229 a          153 c          126 g          200 t
ORIGIN      
        1 aaacagattt atgaatttcg ttcataaccc ctgacaaatt ctgccgtcta atcgagcttt
       61 tccctttacg ttatgcttct catgaacgat aacacaactt gttcatgaat taaccattcc
      121 ggataaacta tgggatcaaa ttcaggcatc gaaaggaact ggctggtttc cgttaatgga
      181 ttaaatatct attattctcc cgagagtaaa actatcaaat caatgagtaa ccgatacagc
      241 ctcacaaatt ctttcagata attaacccgt cactgcggca cgttctctca taaggcgtgc
      301 ctgcagtgta atccagtatg tatccggcat agttgacggt ttaatagaac tcaattcctc
      361 tcccaggtca ccaacaacga tatcttgtat aacaccccag gagatacgac attatgatga
      421 gatgcgatat gctcctcagc accaatcaac gcccggactg ggccagtctt gagatcagac
      481 tcattcccgg aactgacttc aggcgacata tatcaatgac gttggtgcaa taaaagatag
      541 ttgatgtaat acatctattc aaatttgtga tacatgtcaa aaaattggtt gaccaaactc
      601 gttatttata taagggcact tacgaagtgc actctttttt aaagcgagga agtaccaatg
      661 aaagagaata aagtacagca aatcagtcat aaactgatta atatcgtt
//
LOCUS       DBANUU010000456          700 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.463, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000456 DBANUU010000000
VERSION     DBANUU010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.463"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..150
                     /locus_tag="ACIE68_005131"
     CDS             <1..150
                     /locus_tag="ACIE68_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFX5911253.1"
                     /translation="RPPVLNEEQKQVVFERIKSGVSISAIAREFKTSRQTILRAKAKL
                     QTPDI"
     gene            complement(248..370)
                     /locus_tag="ACIE68_005132"
                     /pseudo
     CDS             complement(248..370)
                     /locus_tag="ACIE68_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(422..>700)
                     /locus_tag="ACIE68_005133"
     CDS             complement(422..>700)
                     /locus_tag="ACIE68_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HFX5911254.1"
                     /translation="GSFTHYGEELQGPTVDGNELREETRYLNVDYAAVTGLLVQVARE
                     TDGRVTALEEENITLRKNLATAGTRISTLENQVSELVALVGQLTGSEH"
BASE COUNT          187 a          182 c          141 g          190 t
ORIGIN      
        1 cgaccacctg tgttaaatga agaacagaaa caggtggtat tcgaacgaat taagtcaggt
       61 gtaagtataa gtgccattgc ccgggaattc aaaacctcgc ggcaaaccat tttaagagcc
      121 aaagcaaaac ttcagacacc tgacatataa aaaataatct cggtgtgaga tgctttacgt
      181 cttccaagcc cccttccttg ccgtaaatgg aaagatacat ctaattatag aatttatatg
      241 ttttacccta cggcagtgct ggccattcaa tatcctgtgc agttgacgta tcaacacggt
      301 tcagcaatac ccgatacttt ttccagactt ccagcaacga agtttcttcc tccgttgcga
      361 tttccagctc aacaacagtc tgaacgtacc aggaacagcc tccttcaggg cttgaaggat
      421 atcaatgttc gcttcctgtt aactgcccga caagtgcaac cagttcgctt acctgatttt
      481 ccagagtgct gatccgggtg cctgctgttg ccagattttt acgtagcgtt atgttttcct
      541 cttccagcgc ggtaacgcga ccatctgttt cacgggcgac ctgaacaagt aaccccgtca
      601 cggcggcgta gtcaacattc agataacgtg tttcttcacg taattcattg ccgtcaaccg
      661 ttggtccctg caactcttca ccgtaatgag taaacgatcc
//
LOCUS       DBANUU010000457          697 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.464, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000457 DBANUU010000000
VERSION     DBANUU010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.464"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..523)
                     /gene="fimE"
                     /locus_tag="ACIE68_005134"
     CDS             complement(<1..523)
                     /gene="fimE"
                     /locus_tag="ACIE68_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFX5911255.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTAS"
BASE COUNT          217 a          173 c          138 g          169 t
ORIGIN      
        1 tactggcggt ataacgcaca gtatggcgaa tatttcgatg cccgagataa tcctgaatta
       61 aacgggtatc tgcaccacgc tccgccagtt cataaccgca agcatgcctt aacatatgag
      121 gatgagtctg cgttacggtt ccagcttcaa taccggcatc gcgaataatg cgataggcct
      181 gctggcgaga aagccgactc ccgcggcgag aaataaatat agcgtcggtc cggtcagcac
      241 ctttccagtt agcacgttcc tgggtccagc gttccacagc ttcacgctca tcaaaacgta
      301 acgggtgaac ggtagaaaat ccgttcttca gtcggcgaat atttattcga ccttcattaa
      361 ggtcaagatc ctgataatgc agatcaagca gttcactaat acgcatccca tgccgatatg
      421 ccaacagaat aagacaataa tctctggctc ccgttgcccc gtaacacacc gcctgcatca
      481 tggcctgaac ttctttaccg gtaagataac gacgtttact cacaatagta gtactcctga
      541 ctgagatata tttgaatgaa tacctatagg aaacctcaat cggtcaaaat tagcccaata
      601 gataaaaaga taaccacaca cgaagtgatg tggctatcag tcaattacat taaatatcat
      661 acaaaaataa aatatcactg atgtgacaac atctatt
//
LOCUS       DBANUU010000458          690 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.465, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000458 DBANUU010000000
VERSION     DBANUU010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.465"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            115..355
                     /locus_tag="ACIE68_005135"
                     /pseudo
     CDS             115..355
                     /locus_tag="ACIE68_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            432..690
                     /gene="sctI"
                     /locus_tag="ACIE68_005136"
                     /pseudo
     CDS             432..690
                     /gene="sctI"
                     /locus_tag="ACIE68_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322788.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
BASE COUNT          237 a          115 c          147 g          191 t
ORIGIN      
        1 atgtgaattt gaaaaatcag gacacaaata atagttcatt tagctacgga gataaccgat
       61 tcgagaagtc tcaaggtagc aactggacat ttcaggaata attgaggagg acttatggca
      121 gattggaatg gttatattat ggatatcagc aatcaatttg atcagggcgt tgatgatctg
      181 aaccagcaag ttgaaaaagc gttggaggat ttagccaacc aatccctccg acacgaaatt
      241 tcttgccgaa tatcaaagtg cattagctga gtatacatta tatcgaaatg cgcaatctaa
      301 cgttgtaaaa gcgtataaag atcttgattc agcaatcata caaaacttcc gctaaactgg
      361 ataaggattt tattatgtca gtaagcaata tgccacctat tgatagagct gagtaatcaa
      421 ctgctcatga aatacagcaa gcgaaagtga ttgatttaaa tgaccgggtt ctcaatcttg
      481 acaacccgga tgataaaatg atctccgctt ttgccaacta tgctgtacaa actgaaaatt
      541 ggcagcaaaa tgcgttacaa gcattaaggt ctgataaaga ggggttaact cctgaaaaat
      601 tattagtctt gcaggatcat gtgcttaatt ataatgtcga ggtttcattg gtggaacatt
      661 agcaagaaag attgtcgctg cagttgagac
//
LOCUS       DBANUU010000459          689 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.466, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000459 DBANUU010000000
VERSION     DBANUU010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.466"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            204..629
                     /gene="yqeK"
                     /locus_tag="ACIE68_005137"
     CDS             204..629
                     /gene="yqeK"
                     /locus_tag="ACIE68_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFX5911256.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAENVLTQTT
                     SWTLKSRYVNVNSVNKVNVKSKVKNSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
BASE COUNT          236 a          100 c          127 g          226 t
ORIGIN      
        1 gtggcttcat tttacatttc tctcatttga ttagagcaaa atccgaattc gcaacttctg
       61 ataatgtcaa atatcacctt atggagacgt tacgtagaat gactatattt gttatcggca
      121 cattaggggt attaatttta gtattgatgg aaggtgatta ttgaacagta acatcgagtc
      181 acggtaaaaa tgtgaggtat acaatggaca ttgaattttc gcagattcat gaaatggtct
      241 atatgcatga tattgtaaat tcggactcga aaaagaaacc aaggattccg ttaaaaaaat
      301 tccttaatgc tgagaatgtt ctcacgcaaa caacatcatg gacattaaaa tcaagatatg
      361 tgaatgttaa ttctgtcaat aaagtcaacg taaagagtaa agttaaaaac agttatatct
      421 cacgatctgt caatgatgag tttagtttaa cagatgatga aatcaattct tttaaagaaa
      481 cgttagtcct ttcctctatt gattcactga gtaaactggt tcttaataat cctctttctg
      541 tattgtttac atcgactgta cgaaggaata ataatcgagc taagatgaat gtcgagttcg
      601 actcatggat ttgcaccaga tgttgttgat acatgatatt ttacattgca aggagatgtc
      661 taaaatgaaa gatgttgatc aaaactttg
//
LOCUS       DBANUU010000460          687 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.467, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000460 DBANUU010000000
VERSION     DBANUU010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.467"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>687
                     /locus_tag="ACIE68_005138"
     CDS             <1..>687
                     /locus_tag="ACIE68_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911257.1"
                     /translation="NNKLQPEEVYKESQQTAKLEIFCDEFLKISQSRYGLSTSADSIA
                     NLLTFFTKASDAIDRIKTQKIDVHSYGFVPIRHFVEHVITYKNEFAADYEPYTLTFTR
                     GENNEGVLSIESKEGSISQRTINLNEYETAINIINEHVTKENIHNTVQSLTEKDISKI
                     NSSDKHHKISSEESIKSQLYSDQKKYADLLLHSEKNTEWYKYASSEERYDKFKNSSKE
                     IKNTYKQIVL"
BASE COUNT          287 a          124 c          107 g          169 t
ORIGIN      
        1 aaaataataa attgcaacca gaagaagttt acaaagaatc acaacaaacc gcgaaacttg
       61 agattttttg tgatgagttt ctaaaaatat ctcaaagtag atacggatta tcaacctcag
      121 cagacagcat agctaattta ttgacttttt tcacgaaagc ctcagatgca attgatagaa
      181 tcaagaccca aaaaattgac gttcacagtt atggtttcgt accaataaga cattttgtcg
      241 aacatgtcat aacgtataaa aatgaatttg cagcggatta tgaaccctat acccttacct
      301 ttacccgagg tgaaaacaac gaaggtgttt tgagtatcga aagcaaagaa gggtctattt
      361 cacaaagaac aataaactta aatgaatatg aaactgcaat taacatcatc aatgaacatg
      421 ttacaaaaga aaatatccac aacacggtcc agtcactgac agaaaaagac atttcaaaaa
      481 taaacagctc tgataaacat cataagatca gcagtgagga aagtattaaa agtcaacttt
      541 acagcgatca aaaaaaatat gcagacctgc tactacattc tgagaaaaat acagaatggt
      601 acaaatacgc atcctccgag gaacgctatg ataaattcaa gaattcttct aaggaaatta
      661 aaaacaccta caagcaaatt gtactag
//
LOCUS       DBANUU010000461          686 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.468, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000461 DBANUU010000000
VERSION     DBANUU010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.468"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..599
                     /gene="ydeO"
                     /locus_tag="ACIE68_005139"
     CDS             <1..599
                     /gene="ydeO"
                     /locus_tag="ACIE68_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFX5911258.1"
                     /translation="NTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSE
                     ERNHHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDIC
                     DCLYISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYF
                     IYAFRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
BASE COUNT          210 a          140 c          139 g          197 t
ORIGIN      
        1 aaaataccat taaaaaatat ctacaatgca ctaacatcca gacagtgacg gtgcctgttc
       61 ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt gcttaatgaa atgattgctt
      121 atctcaactc ggaagaacgc aatcatcata atttttcaga acttttgctt ttttcttgcc
      181 tgtctatttt tgccgcatgc aaaggtttca ttacactatt aactaacggt gtgctatccg
      241 tttccggaaa agtgagaaat attgtcaaca tgaagctggc gcacccatgg aagctgaaag
      301 atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa gaaaaaacta aagcaagagc
      361 aaacgacatt ctcacagatt ctattagatg ccagaatgca gcacgcaaaa aatttgatac
      421 gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg ttatgccagt acatcttatt
      481 ttatttatgc gttccgcaaa catttcggca acagtccgaa gagagtttct aaggagtacc
      541 gttgtcaaag tcacacgggt atgaatacgg gcaacacgat gagtgcttta gctatttgat
      601 tatttgctaa cgagtagtca accacgcacg ctacgtaaga attatatggg gcagccattc
      661 cctgccccgc gttgttttta ggcgat
//
LOCUS       DBANUU010000462          680 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.469, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000462 DBANUU010000000
VERSION     DBANUU010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.469"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>680
                     /locus_tag="ACIE68_005140"
     CDS             <1..>680
                     /locus_tag="ACIE68_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911259.1"
                     /translation="DFTPGMINTYHGDIYNRTTDTDNVKTPDTPMWPCDNREEQQPIN
                     STFSGEGYNPEQYDLARQHLPQIYACRTNTKYTDADYSKVVAQLVSLITNIESISSTP
                     LTRQAQEILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPVRFSVQTM
                     FSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNMTGQ
                     NLSGFVLT"
BASE COUNT          252 a          144 c          118 g          166 t
ORIGIN      
        1 ttgactttac cccaggtatg ataaatacat atcacggcga tatttataac cgcacaacag
       61 ataccgacaa tgttaaaaca ccagacacac cgatgtggcc ttgtgataat cgggaagaac
      121 aacaacccat taattccaca ttctctggag aaggatacaa ccctgaacaa tatgatttag
      181 cgcggcaaca cctcccacaa atttatgcgt gccgcacgaa caccaaatat acagatgcag
      241 actactcaaa agttgtggct caacttgtaa gtcttattac taacattgaa tcgataagtt
      301 cgacaccact tacgcggcag gcacaggaaa tacttaatca aattaataac attagatacg
      361 aaaagaacaa aagtgctgaa tgtcgaatca ttgttgtcgc taatcctaaa cccgacaagg
      421 caatcataac taaaattagc gtagaagagg gaataccagt aagattcagt gtccaaacca
      481 tgttttcaga tactaatttt attgctgaac aacgagctga cttaccgact aatataaaag
      541 atattcaaag cctgtatcaa aaaatgacga agttatatat tgagcacagt gaaaataaaa
      601 acaggatgaa agtctttgcc ggaaccaact ttattgattt caatatgaca ggacagaacc
      661 tgtccgggtt cgttctaacc
//
LOCUS       DBANUU010000463          677 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.470, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000463 DBANUU010000000
VERSION     DBANUU010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.470"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..307
                     /locus_tag="ACIE68_005141"
     CDS             <1..307
                     /locus_tag="ACIE68_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFX5911260.1"
                     /translation="ICHYRAADAADYVRETDRSLMQLVTALWATLALMYVSLAAITGK
                     YPVLPGKMKCIRVVTADEYLKDVYTEDASLPGKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            294..614
                     /locus_tag="ACIE68_005142"
     CDS             294..614
                     /locus_tag="ACIE68_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFX5911261.1"
                     /translation="MNMRNINVITARVVPDKNVSDDFMHAVLSNCTTKIVLPAPKVFS
                     SESLPHNFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKTNLTAELISAVVGSNKK
                     GEGQ"
     gene            611..>677
                     /locus_tag="ACIE68_005143"
     CDS             611..>677
                     /locus_tag="ACIE68_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraV"
                     /protein_id="HFX5911262.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGT"
BASE COUNT          192 a          141 c          167 g          177 t
ORIGIN      
        1 tatctgccat taccgcgcgg ctgatgctgc agattatgtc agggagactg accggtcact
       61 gatgcaactg gtcaccgcat tatgggcaac actggcactg atgtatgtct cgctggcagc
      121 gatcaccggg aaatatccgg tgctgcccgg aaaaatgaaa tgtatccggg tggtaaccgc
      181 agatgaatat ctgaaggacg tttatacgga agacgcttcc ctgccgggaa aaatcaggaa
      241 atgccatgtt tatttccctg atgaccgaac aaacagaagc aacggagata aaaatgaaca
      301 tgcgtaatat taatgttatt acagcccgcg ttgtgccgga taaaaacgta tcagatgatt
      361 ttatgcatgc tgttctcagt aactgcacca caaaaatagt tcttcctgct ccgaaggtgt
      421 tcagttctga atcattgccg cacaatttta atatggctgc tgtcggtgtg atgaagaagg
      481 gaatgtcgat gagcttattt tcttctccgg gaaatacagt ggtctccgga catccctgtg
      541 cagggaaaac aaatctgaca gcagagttaa tttctgctgt tgtggggagt aataaaaaag
      601 gagaagggca atgaaacaga tttctttatt tattcctctg ctgggaacct tattactttc
      661 cggctgcgca ggaacaa
//
LOCUS       DBANUU010000464          676 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.471, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000464 DBANUU010000000
VERSION     DBANUU010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.471"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..96
                     /locus_tag="ACIE68_005144"
     CDS             <1..96
                     /locus_tag="ACIE68_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFX5911263.1"
                     /translation="AVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
BASE COUNT          215 a          115 c          148 g          198 t
ORIGIN      
        1 gctgtaaata acggtaatat tgatgcaaag agcaaacttc aagagctcaa aaagctaaag
       61 cgaatggata gaaggaagaa taaggagaat ccgtaaaagc aatagttcat cagactgccg
      121 cagcagtagt atgatgatga atcagggctg tactataact attatcggta acattaaccg
      181 cagcaggggc ggtataacac tcaggctccg attgggctgg aaggtaggtg gaatttttat
      241 cagtatccgt tgaacccgat ttctggaatc gatccccttg ggattagcca cctgtttata
      301 cagtataact ttaggtatgc tttcgtgtgt ttcagatata ccatgtgatg ataattttta
      361 tgatgtgctt agtattccag tcgcttctgg taataatatg caatgtaaaa ataatccccg
      421 ttgtacacat ttataaatag atggccaata cctcaaggcc tatggagttg gaatgttaat
      481 gacccgggag caagcaatag aaaacctgac ggcatacgat tagtaccgtc agttaataca
      541 ggaacttata atcgcaatgg attttcaata catagttgtt taaacgcttt tggtccgtct
      601 ttaggacctc gattctgttc cgagggttgt ataactggtt tatctaatga catgcaaaaa
      661 ttaaatgagt taatat
//
LOCUS       DBANUU010000465          675 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.472, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000465 DBANUU010000000
VERSION     DBANUU010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.472"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            15..143
                     /locus_tag="ACIE68_005145"
                     /pseudo
     CDS             15..143
                     /locus_tag="ACIE68_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            128..675
                     /locus_tag="ACIE68_005146"
                     /pseudo
     CDS             128..675
                     /locus_tag="ACIE68_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
BASE COUNT          210 a          112 c          149 g          204 t
ORIGIN      
        1 taaggagatg aaacatgatt gaagaagggc tgttacttcc aatgaatgtt ttgcttcaag
       61 gacagaagtt aaccctactg gaccctgaaa tgtggttctt acggggaaat gaaaacaagg
      121 atgtaactat attcttgatc aattagagat gaagtatcct gatctattaa aagagatatt
      181 aagatatata accgtgcagc gcatttcaga ggtaattcag cgtctgatcc tggagcgaat
      241 ttcagtgcgc aatatgcggt tagttatgga ggccttagca ttatggagcc cacgagagaa
      301 agatataatc acactcgtcg agcatgtacg aggtgcatta ggtcgctaca tttgccataa
      361 gttttcatat tctggagaaa ttaaagcaat tgtgatttcg cctgaaatag aggacagaat
      421 cagagatggt gtcaggccta ccgctggcgg tacttttctt aatctcgatg catccgaagc
      481 tgagatgatt ctggataatt ttaaactggc actttctgga atcaatattc ctattaaaga
      541 cattatttta ctgggatctg tggatattcg tcgttttatc aaaaaactta ttgaatctag
      601 ttatcgagat ctcgaagtac tttcgtacgg ggaactaacg gaaaatgttc ccgttaatat
      661 tctgaaaact attta
//
LOCUS       DBANUU010000466          675 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.473, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000466 DBANUU010000000
VERSION     DBANUU010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.473"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            110..346
                     /gene="ycgZ"
                     /locus_tag="ACIE68_005147"
     CDS             110..346
                     /gene="ycgZ"
                     /locus_tag="ACIE68_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFX5911264.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            389..661
                     /locus_tag="ACIE68_005148"
     CDS             389..661
                     /locus_tag="ACIE68_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFX5911265.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRIKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
BASE COUNT          236 a          114 c          134 g          191 t
ORIGIN      
        1 ttagcactaa atgcaaaaaa ataatttatc attctgtaca catatttcgt acaagtttgc
       61 tattgttacc tcacttaaca ttgattaaca tttttaacag aggcgtagca tgcatcaaaa
      121 ttcagtgact ttagattctg cgggagcaat cactcgttac tttgcaaaag ctaacttgcc
      181 tactcagcag gaaactctcg gggaaattgt gactgagatt ttgaaagatg gacgtaatct
      241 gagtcgaaag tcgctttgtg ccaaacttct atgccgactg gaacaagcga caggggaaga
      301 ggaacagaaa cattataatg cactaattgg gttgcttttt gaataatgca acagcctgat
      361 tatttaacag gttagctatg aagtcgttat gaagacatct gataatgaac gtataaaata
      421 tgaaattact ggccaggcgg tgctccagat actgcgcata aagataaatt tttcattgca
      481 gactcttatt aagcaattac tcgtaatgaa atcggctgag gaagatgctt tccgacgcga
      541 tttaattgac agcataattc gtgattttag caatagtgat tcaggagggc caaaccgaag
      601 aacagcaaca gctgacaata aaagtatgtt caatggtaag aaaataaaca gaatacatta
      661 aaatttcata agtaa
//
LOCUS       DBANUU010000467          671 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.474, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000467 DBANUU010000000
VERSION     DBANUU010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.474"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>671
                     /locus_tag="ACIE68_005149"
     rRNA            <1..>671
                     /locus_tag="ACIE68_005149"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          156 a          148 c          224 g          143 t
ORIGIN      
        1 acagtgtctg gtgggtagtt tgactggggc ggtctcctcc taaagagtaa cggaggagca
       61 cgaaggttgg ctaatcctgg tcggacatca ggaggttagt gcaatggcat aagccagctt
      121 gactgcgagc gtgacggcgc gagcaggtgc gaaagcaggt catagtgatc cggtggttct
      181 gaatggaagg gccatcgctc aacggataaa aggtactccg gggataacag gctgataccg
      241 cccaagagtt catatcgacg gcggtgtttg gcacctcgat gtcggctcat cacatcctgg
      301 ggctgaagta ggtcccaagg gtatggctgt tcgccattta aagtggtacg cgagctgggt
      361 ttagaacgtc gtgagacagt tcggtcccta tctgccgtgg gcgctggaga actgaggggg
      421 gctgctccta gtacgagagg accggagtgg acgcatcact ggtgttcggg ttgtcatgcc
      481 aatggcactg cccggtagct aaatgcggaa gagataagtg ctgaaagcat ctaagcacga
      541 aacttgcccc gagatgagtt ctccctgact ccttgagagt cctgaaggaa cgttgaagac
      601 gacgacgttg ataggccggg tgtgtaagcg cagcgatgcg ttgagctaac cggtactaat
      661 gaaccgtgag g
//
LOCUS       DBANUU010000468          664 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.475, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000468 DBANUU010000000
VERSION     DBANUU010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.475"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            65..649
                     /locus_tag="ACIE68_005150"
     CDS             65..649
                     /locus_tag="ACIE68_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5911266.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
BASE COUNT          190 a          140 c          142 g          192 t
ORIGIN      
        1 gtgtcatgaa tagtcattct tcaaaccagt agtctgacta atggaatttt attcaaggat
       61 ttttatgtct aaaaaattag gttttgccct gtctggtctt atgctggcaa tggttgcagg
      121 tactgcttct gctgatatgg atggtggtca gttaaatatc agtggtctgg ttgttgataa
      181 cacctgtgaa acccgcgttg acggcggtaa taaagatggt ctgatcctgc tgcaaactgc
      241 aactgttggt gaaatcgatg ctggcgtgct gaatgacacc gttggcgcta aagccaaacc
      301 tttcagcatc accgttgact gcagcaaagc aaatccgaat ccaggtagta cagctaaaat
      361 gaccttcggt tctgttttct ttggtaatag caaaggaacg ctgaacaacg acatgtctat
      421 taacaacccg tctgacggcg tgaatatcgc tctgcacaat attgatggtt ccactatcaa
      481 acaggttcaa attaacaacc ctggcgatgt gtatactaaa gccctggatg caacgacaaa
      541 atctgctgtt tatgatttta aagcgtctta cgttcgtgcc gtcgcagacc aaacagcaac
      601 tgctggttat gtaaaaacta acactgcata caccattact tatcagtaag tattcaccaa
      661 ttaa
//
LOCUS       DBANUU010000469          653 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.476, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000469 DBANUU010000000
VERSION     DBANUU010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.476"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>653
                     /gene="yfdF"
                     /locus_tag="ACIE68_005151"
     CDS             <1..>653
                     /gene="yfdF"
                     /locus_tag="ACIE68_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFX5911267.1"
                     /translation="SNDTDHITIGIDNQEPIELPANYKDKELVRTIINDNIVEKTHDI
                     NNKEMIFSALKEIYDGDPGFIFDKISHKFRHTVTEFDESGNSEPTDLFTWYGKDKKGD
                     SLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARNASAWFESSKA
                     IMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKFRRDNVKEIIS
                     "
BASE COUNT          246 a          113 c          129 g          165 t
ORIGIN      
        1 tccaatgata cagaccatat tactattggt atcgacaacc aagaaccaat agaattacct
       61 gcgaactata aagacaaaga actcgtccgt actatcatta atgacaacat agttgagaag
      121 actcatgata tcaataacaa ggaaatgatc ttcagcgcat taaaagaaat atatgatgga
      181 gatcctggtt ttatcttcga taagatatca cacaaattca gacatacggt aacggaattt
      241 gatgagagcg ggaatagcga accaacggac ttatttacct ggtacggtaa agataaaaaa
      301 ggcgactctc tcgctattgt aattaaaaat aaaaacggaa atgattactt atctctcggt
      361 tactacgatc aggacgacta ccacattcaa agaggaattc gtattaatgg tgatagtctc
      421 acccaatatt gtagtgaaaa agccaggaat gcttcagcgt ggtttgaaag cagtaaagct
      481 atcatggcag aatcatttgc aacaggttcc gatcatcagg ttgtaaacga gctcaacggg
      541 gaaagactga gagaacctaa cgaagttttt aaacgtttag gtcgagcaat aagatataat
      601 tttcaagtgg acgatgcaaa atttagacgc gacaatgtaa aagaaataat ttc
//
LOCUS       DBANUU010000470          651 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.477, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000470 DBANUU010000000
VERSION     DBANUU010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.477"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..434)
                     /locus_tag="ACIE68_005152"
     CDS             complement(<1..434)
                     /locus_tag="ACIE68_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFX5911268.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTISVIMLVGTLYLGYV
                     FDVHHIMSILEYIFDHNAKPDIWDYFKAMGVGWLKTIIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFKFDSLNPVNGLKRIFGLKTVKEFVKAILYIILF"
     gene            complement(443..649)
                     /locus_tag="ACIE68_005153"
     CDS             complement(443..649)
                     /locus_tag="ACIE68_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFX5911269.1"
                     /translation="MIAVMLGGEAVLGLLARYASQLNAFAISLTVKSALAFLILIIYF
                     GPILAERVMPLSFFPEQLQLYIEK"
BASE COUNT          225 a          122 c          109 g          195 t
ORIGIN      
        1 aaaaataaaa taatataaag aattgcttta acaaattctt ttacggtttt taagccaaat
       61 atacgcttta agccatttac tggattcaat gaatcaaact taaatttaac agcttcagtt
      121 gctaattgca ttttactttg aaaccaggag acaagtattg ttgtgaacat gcaaaccagc
      181 aaaaaaggaa tgatcgtttt caaccaacca acccccatag ctttaaaata gtcccaaata
      241 tctggtttag cgttatgatc aaagatgtat tcaagaatcg acataatgtg atgcacatca
      301 aaaacatatc cgagatataa agtacccaca agcattatta cagagatggt taagtctcta
      361 ctttttagaa tttgcccttt tttagaagca tcctggagtt tcttttgtgt tggcttttca
      421 gttttattgg ccatgaaaca ctctatttct caatgtatag ttgcaattgt tctgggaaaa
      481 aggataacgg cattactcgt tcagccagta ttgggccaaa ataaataatt aatattaaaa
      541 atgccagcgc acttttgaca gtcagtgaaa tggcaaaagc atttagttgt gaagcataac
      601 gtgccagcaa tcccaggaca gcctcaccgc ccagcataac ggcaatcacg g
//
LOCUS       DBANUU010000471          651 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.478, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000471 DBANUU010000000
VERSION     DBANUU010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.478"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            1..651
                     /locus_tag="ACIE68_005154"
                     /pseudo
     CDS             1..651
                     /locus_tag="ACIE68_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          150 a          178 c          193 g          130 t
ORIGIN      
        1 agcagacgtc ttctgaacgt gaactggaga tgtctaccga gattgcacgt ctcaaacgcc
       61 agctggcaga acgggatgaa gagctggcta tcctccaaaa ggccgcgaca tacttcgcga
      121 agcgcctgaa atgaagtatg tctttattga aaaacatcag gctgagttca gcatcaaagc
      181 aatgtgccgc gtgctccggg tggcccgcag cggctggtat acgtggtgtc agcggcggac
      241 aaggataagc acgcgtcagc agttccgcca acactgcgac agcgttgtcc tcgcggcttt
      301 tacccggtca aaacagcgtt acggtgcccc acgcctgacg gatgaactgc gtgctcaggg
      361 ttaccccttt aacgtaaaaa ccgtggcggc aagcctgcgc cgtcagggac tgagggcaaa
      421 ggcctcccgg aagttcagcc cggtcagcta ccgcgcacac ggcctgcctg tgtcagaaaa
      481 tctgttggag caggattttt acgccagtgg cccgaaccag aagtgggcag gagacatcac
      541 gtacttacgt acagatgaag gctggctgta tctggcagtg gtcattgacc tgtggtcacg
      601 tgccgttatt ggctggtcaa tgtcgccacg catgacggcg caactggcct g
//
LOCUS       DBANUU010000472          649 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.479, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000472 DBANUU010000000
VERSION     DBANUU010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.479"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..150
                     /locus_tag="ACIE68_005155"
     CDS             <1..150
                     /locus_tag="ACIE68_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
                     /protein_id="HFX5911270.1"
                     /translation="TFLMLFLVSLVLISAKIKSNGLLMPILLHMSMNAVIAGIQYLAS
                     ISYTH"
     gene            complement(325..603)
                     /gene="mcmA"
                     /locus_tag="ACIE68_005156"
     CDS             complement(325..603)
                     /gene="mcmA"
                     /locus_tag="ACIE68_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin McmA"
                     /protein_id="HFX5911271.1"
                     /translation="MRKLSENEIKQISGGDGDDGQAELIAIGSLAGTFISPGFGSIAG
                     AYIGDKVHSWATTATVSPSMSPSGIGLSSQFGSGRGTSSASSSAGSGS"
BASE COUNT          200 a          140 c          113 g          196 t
ORIGIN      
        1 acatttttga tgctctttct ggtatcgtta gttcttatta gtgcaaaaat aaagtcgaat
       61 ggcctgttaa tgcccatatt acttcacatg tcgatgaatg cagtcatagc aggaatacaa
      121 tatctggctt ctatcagtta tactcattaa cagagtattt tctgttacaa tctcatatga
      181 taaaaattaa acggactggc attgttacta tgcggtatgc ataaaataat aaacgttcgg
      241 aggagcctaa cttaaagcgt tacataggca ccattatcat ataatgaagc acctgtaaca
      301 tgtgcttcat taacaatata aggtttaact tccactcccc gcagacgaag aggcacttga
      361 tgtacctctg ccggatccaa actgggatga taatcctata cctgagggag acatggaggg
      421 actaaccgtc gcagtcgttg cccatgaatg tactttatca cctatataag cccctgcaat
      481 agaaccaaat cccgggctaa taaacgtacc tgcaagtgaa ccaatagcaa ttaattctgc
      541 ctgcccgtca tccccgtcac ctccagatat ttgttttatt tcattttcag atagttttct
      601 catttcaaca ccttcgctat aagatttgtg aagaagtaaa aattcacgt
//
LOCUS       DBANUU010000473          649 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.480, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000473 DBANUU010000000
VERSION     DBANUU010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.480"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            200..>649
                     /locus_tag="ACIE68_005157"
     CDS             200..>649
                     /locus_tag="ACIE68_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFX5911272.1"
                     /translation="MKYIKSSSLLALTLLFNSGFVNADNKQTLIEAATAGDTAAQSEL
                     GTNYFDGVNGFDKDVVEAKKWIDLAAEKGDKVAYYALGVMYTFGEGVDKDLNKAVEYY
                     KLAGDAREGRAYNNLGAIYQKGMLGKVDHALAIKYFKLASDAGYVKAT"
BASE COUNT          216 a          117 c          133 g          183 t
ORIGIN      
        1 attagttaat caaaacacga gcagtgccac aataattaaa tggcgtacta tttcttatgg
       61 ttattagttt tccagtaact ttaccgacca taaactaaat caacaaagct gacatattaa
      121 ctaaatttaa gtaccctttt tcgcacactt gacgcttgcc gcgtctaaac tgttcatcct
      181 atatcaacag aaaaaaaaca tgaaatacat aaaatcttca tcgttgctgg cattaacttt
      241 gctttttaac tctggattcg ttaacgcaga caataaacaa acgctgatag aagccgctac
      301 cgcaggggat accgcagcac aaagtgagtt aggtacaaat tattttgatg gtgtcaatgg
      361 ttttgataag gatgtagttg aggccaagaa gtggattgat ctagctgcag aaaagggaga
      421 taaagtagca tattatgcac ttggagttat gtacacattt ggcgagggtg tagataaaga
      481 cctaaataaa gctgttgagt actacaaact tgcgggggat gctcgtgaag gtagagccta
      541 caacaatctt ggtgctattt atcaaaaggg gatgcttggt aaagtagatc atgcactcgc
      601 cattaaatac ttcaaactag cctctgatgc cgggtatgtt aaagcaact
//
LOCUS       DBANUU010000474          647 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.481, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000474 DBANUU010000000
VERSION     DBANUU010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.481"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..252
                     /locus_tag="ACIE68_005158"
     CDS             <1..252
                     /locus_tag="ACIE68_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFX5911273.1"
                     /translation="MTVTPASTSVVKGQSTTLTVAFQPEGVTDKSFRAVSADKTKATV
                     SVSGMTITVNGVAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            268..>647
                     /locus_tag="ACIE68_005159"
     CDS             268..>647
                     /locus_tag="ACIE68_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFX5911274.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGP"
BASE COUNT          156 a          167 c          192 g          132 t
ORIGIN      
        1 atgaccgtga cgcctgccag cacctcggtg gtgaaagggc agagcaccac gctgaccgtg
       61 gccttccagc cggagggcgt aaccgacaag agctttcgtg cggtgtctgc ggataaaaca
      121 aaagccaccg tgtcggtcag tggtatgacc atcaccgtga acggcgttgc tgcaggcaag
      181 gtcaacattc cggttgtatc cggtaatggt gagtttgctg cggttgcaga aattaccgtc
      241 accgccagtt aatccggaga gtcagcgatg ttcctgaaaa ccgaatcatt tgaacataac
      301 ggtgtgaccg tcacgctttc tgaactgtca gccctgcagc gcattgagca tctcgccctg
      361 atgaaacggc aggcagaaca ggcggagtca gacagcaacc ggaagtttac tgtggaagac
      421 gccatcagaa ccggcgcgtt tctggtggcg atgtccctgt ggcataacca tccgcagaag
      481 acgcagatgc cgtccatgaa tgaagccgtt aaacagattg agcaggaagt gcttaccacc
      541 tggcccacgg aggcaatttc tcatgctgaa aacgtggtgt accggctgtc tggtatgtat
      601 gagtttgtgg tgaatgatgc ccctgaacag gcagaggacg ccgggcc
//
LOCUS       DBANUU010000475          647 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.482, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000475 DBANUU010000000
VERSION     DBANUU010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.482"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>647
                     /locus_tag="ACIE68_005160"
     CDS             <1..>647
                     /locus_tag="ACIE68_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HFX5911275.1"
                     /translation="PHSPSHWMRTLCDVDFTPPVDGETLETHAPAFWRWLDRAAGGRA
                     EKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKSIMAEIATLLAGEDNATSATIETL
                     ESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITGGDAVSVDPKYRDAYSTHIPAVIL
                     AVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERDPQLKDKITRELAVIVRHLMQ"
BASE COUNT          130 a          209 c          201 g          107 t
ORIGIN      
        1 ccgcacagtc cgtcacactg gatgcgcacc ctgtgcgatg tggatttcac cccgccggtg
       61 gacggtgaaa cgctggagac ccacgccccc gcgttctggc gctggcttga ccgtgccgcc
      121 ggtggccgtg cggaaaaacg cgacgtgatt ctggccgcac tgtttatggt gctggcaaac
      181 cgctacgact ggcagctctt tctggaggtg accggtcccg gcggcagcgg caaaagtatc
      241 atggccgaaa tagccaccct gctggccggg gaggataacg ccacgtcggc caccatcgag
      301 acgctggaat ccccgcgtga acgtgccgcg ttaactggct tctcactgat acgcctgccg
      361 gaccaggaaa aatggagcgg cgacggagcc ggactcaagg ccatcaccgg cggcgatgca
      421 gtgtccgttg acccgaaata ccgggatgcg tactccacgc acatcccggc ggtgattctg
      481 gccgtgaaca ataacccgat gcgcttcacc gaccgcagcg gcggcgtgtc acgccggcgg
      541 gtgattattc acttcccgga acagatagcc ccgcaggagc gcgacccgca gcttaaggac
      601 aaaatcaccc gcgagctggc ggtcatcgtg cgtcacctga tgcagaa
//
LOCUS       DBANUU010000476          647 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.483, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000476 DBANUU010000000
VERSION     DBANUU010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.483"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>647
                     /locus_tag="ACIE68_005161"
     CDS             <1..>647
                     /locus_tag="ACIE68_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HFX5911276.1"
                     /translation="PHSPSHWMRTLCDVDFTPPVEGETLETHAPAFWRWLDRAAGGRA
                     EKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKSIMAEIATLLAGEDNATSATIETL
                     ESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITGGDAVSVDPKYRDAYSTHIPAVIL
                     AVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERDPQLKDKITRELAVIVRHLMQ"
BASE COUNT          133 a          203 c          199 g          112 t
ORIGIN      
        1 ccgcacagtc cgtcacactg gatgcgtacc ctgtgcgatg tggatttcac cccgccggtg
       61 gaaggggaaa cgctggaaac ccacgccccc gcgttctggc gctggcttga ccgtgctgcc
      121 ggtggtcgtg cggaaaaacg cgacgtaatt ctggccgcac tgtttatggt gctggcaaac
      181 cgctacgact ggcagctctt tctggaggtg accggtcccg gcggcagcgg caaaagtatc
      241 atggccgaaa tagccaccct gctggccggg gaggataacg ccacgtcggc caccattgag
      301 acgctggaat ccccgcgtga acgtgccgcg ttaactggct tctcactgat acgcctgccg
      361 gaccaggaaa aatggagcgg cgacggtgcc ggactcaagg ccatcaccgg cggcgatgcg
      421 gtgtccgttg acccgaaata ccgggatgca tactccacgc atatcccggc ggtaattctg
      481 gccgtgaaca ataacccgat gcgcttcacc gaccgcagcg gcggcgtgtc acgccggcgg
      541 gtgattattc acttcccgga acagatagcc ccgcaggagc gcgacccgca gcttaaggac
      601 aaaatcaccc gcgagctggc ggtcatcgtg cgtcacctga tgcagaa
//
LOCUS       DBANUU010000477          629 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.484, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000477 DBANUU010000000
VERSION     DBANUU010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.484"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>629)
                     /locus_tag="ACIE68_005162"
     CDS             complement(<1..>629)
                     /locus_tag="ACIE68_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFX5911277.1"
                     /translation="VNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNKDA
                     TPIERCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHDDN
                     SLKLFASLLISLSYSSAYTDLAGAEKVNINEYNENYLTAQFEELSQRDMKKYLGEMKH
                     LADRGGMKFDDYLDKMSLLVNEGKLEPDILSKMRDAAPKLIDFAKSFDP"
BASE COUNT          194 a          137 c          102 g          196 t
ORIGIN      
        1 ttgggtcaaa cgacttagca aagtcaatta atttcggtgc agcatctcgc attttgctta
       61 aaatatcagg ttcgagcttt ccttcattca ccagaagtga cattttatcc agatagtcat
      121 caaatttcat ccccccccgg tccgccagat gcttcatctc tcccaggtac ttcttcatat
      181 cacgttgaga taattcttca aactgagctg tcagatagtt ctcattatat tcattaatat
      241 tcactttttc tgctccggct aaatctgtgt aggcactaga atatgaaagt gaaattaaca
      301 gcgaagcaaa taattttaag ctgttgtcat catgttcctg gcaggcatta acaaacgtta
      361 aaaaaccaga acctattgct tgaaaatgta tattcgctaa aggttcatta tccttagatt
      421 cctcataaaa tagctgaacc gtggagggaa ggccattctt agtttctaca tcaaaagtac
      481 atcgttcaat gggagtcgca tctttattat ccccaggatt acatatacta ttaatcgcga
      541 aatgaaaaat agcccggtca acaactgatg caataaagtc acgatcgttt aaatcctcat
      601 cagtgcaatc gtcaatatac ttattgacc
//
LOCUS       DBANUU010000478          615 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.485, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000478 DBANUU010000000
VERSION     DBANUU010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.485"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..401)
                     /locus_tag="ACIE68_005163"
     CDS             complement(<1..401)
                     /locus_tag="ACIE68_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFX5911278.1"
                     /translation="MMMPALQKLPFSGLPFSGICGYSFSAAAKSAAGRENPSNLTATY
                     DAPCVFFCVAINATERQIMVWRVVCRAGMILFAIACYATESMVAQAGQPPGWPVSVRA
                     GILTPVWAIAIERENSGDSVTCYLTEAAIMAT"
     gene            complement(398..>615)
                     /locus_tag="ACIE68_005164"
     CDS             complement(398..>615)
                     /locus_tag="ACIE68_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFX5911279.1"
                     /translation="PLPDITQERFLRLPEVMHLCGLSRSTIYELIRKGEFPPQVSLGG
                     KNVAWLHSEVTAWMAGRIAGRKRGYDA"
BASE COUNT          146 a          179 c          172 g          118 t
ORIGIN      
        1 gtagccatga tggcagcctc cgttagatag caggttacgc tatcgccgga gttctcacgc
       61 tcgatggcga tagcccagac gggggtgaga ataccggctc taacggatac cggccagccc
      121 ggaggctgcc ccgcctgagc taccattgac tcagtggcat aacatgcgat tgcgaacagg
      181 atcatacctg cacggcaaac cacacgccac accataatct ggcgctctgt ggcgttgatt
      241 gcgacacaaa aaaagacgca tggcgcgtca tatgtcgccg ttagattgct cgggttctca
      301 cgcccggctg ccgattttgc ggcagcggaa aaactatatc cgcaaatgcc ggaaaaaggc
      361 aagccagaaa aagggagttt ttgcagagcg ggcatcatca tgcgtcgtac ccccgtttgc
      421 gtccggcaat gcgtccggcc atccatgcgg tgacttcaga gtgcagccag gccacatttt
      481 taccgccaag actcacctgc ggcggaaatt cccccttacg gatgagttca tagatggtcg
      541 agcgtgacag gccgcacagg tgcatcactt ccggcagacg taaaaaacgc tcctgcgtga
      601 tgtccggcag cggca
//
LOCUS       DBANUU010000479          613 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.486, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000479 DBANUU010000000
VERSION     DBANUU010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.486"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>613
                     /gene="espL4"
                     /locus_tag="ACIE68_005165"
     CDS             <1..>613
                     /gene="espL4"
                     /locus_tag="ACIE68_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HFX5911280.1"
                     /translation="DDYSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAACGDFGLVM
                     KAYFDKMESNGISVMAAILLVDNHALTARLRIKNTTEGCTHYVVSVYDPNVTNDKIRI
                     MSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLMLKGSVDEIT
                     PPLSPATMNLLMAIGQNHQLTQLMIQLQKMPELHRTEMLTAYNS"
BASE COUNT          203 a          115 c          124 g          171 t
ORIGIN      
        1 gatgattact cttcagctga aaaatttctc acgcacttca acaaaaaatg caaaaaccag
       61 actttggcgt tgatttccag ccgtcctgag gggcgttgtg ttgctgcctg cggtgatttc
      121 gggctagtta tgaaggcata ttttgacaag atggaatcaa atggcatcag tgttatggct
      181 gccatattat tggtagataa ccatgctttg acggcccgac taagaataaa gaacacaact
      241 gaaggatgta cccattacgt ggtttcggtt tatgatccta atgtaactaa cgataaaata
      301 agaattatga gcgaaagcaa agaggatatt aaacactatt ctctgatgga ttttatgaat
      361 gtagattata gcctcctgaa atggtcaaat gatcatgtta ttaaccaatc tgttgcaata
      421 attccagcac ttccgaaaga acagctattg atgttaaaag gatctgtgga tgaaataacc
      481 cctccattat caccagcaac gatgaatttg ctaatggcaa ttggtcagaa tcaccaactt
      541 acgcaactga tgattcagct ccagaaaatg ccagaactac atagaacaga aatgttgact
      601 gcctataata gtg
//
LOCUS       DBANUU010000480          612 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.487, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000480 DBANUU010000000
VERSION     DBANUU010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.487"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..401)
                     /gene="mobF"
                     /locus_tag="ACIE68_005166"
     CDS             complement(<1..401)
                     /gene="mobF"
                     /locus_tag="ACIE68_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HFX5911281.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGKGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETV"
     gene            complement(401..>612)
                     /locus_tag="ACIE68_005167"
     CDS             complement(401..>612)
                     /locus_tag="ACIE68_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911282.1"
                     /translation="ELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQQENHPDIQQQ
                     MQRREEVNINVHRERGEDVEPGDDF"
BASE COUNT          116 a          204 c          137 g          155 t
ORIGIN      
        1 accgtttctg actgtccgtc cgtcatcacc cgtgtggagg ccagcgcctc cacctgacga
       61 acagcaaagt ccacggcctg gttatgtgca tcaatcaggc gcttatcgcc acctaacatg
      121 gccatcatgg agacactttt gggggcggag aaggtcaggt cgtagccggg acgatgctta
      181 ttactgccat cctgcatgcg gcttagatcc gctccgtccg gcagccttcc ctccagaaga
      241 cgggtaaaaa catccttatc gacgctgccc tgcagcccaa gctgttcagc ccccttgccg
      301 gcccagcgtt ctcccatgct gcccagtaca tagtaattat ccttgtcggt gtaatagtta
      361 ccggcacttc cggccgatct gacctgcgcg atactcatca tcagaaatca tctcccggct
      421 caacatcctc cccacgctcc cggtgcacat taatgttcac ctcttcacga cgctgcatct
      481 gctgctggat gtccggatga ttttcctgtt gccatgcctc ataagcggcc atatccacca
      541 cttcaccgga ttcactgatc ccgggtggca gttgctgttc catctcttct tccggtttca
      601 ttttcagctc ct
//
LOCUS       DBANUU010000481          610 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.488, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000481 DBANUU010000000
VERSION     DBANUU010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.488"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     repeat_region   77..532
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=77..105
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT          148 a          157 c          178 g          127 t
ORIGIN      
        1 gtttgtgtga tactgtaaag ttggtagatt gtgactggct taaaaaatca ttaattaata
       61 ataggttatg tttacagagt tccccgcgcc agcggggata aaccgtccac gctgtaacgg
      121 ccatcattaa gtttagtgag ttccccgcgc cagcggggat aaaccggctg atggtctggg
      181 agtgtccatc gggcaactga gttccccgcg ccagcgggga taaaccggaa gtaggcctga
      241 cagtgattga acgcatactg agttccccgc gccagcgggg ataaaccgag ttggggcggc
      301 gcaataacga gacgatacgc gagttccccg cgccagcggg gataaaccgg ggagtggcac
      361 ttctggggta gcggcggccc tgagttcccc gcgccagcgg ggataaaccg tcaacgcgct
      421 cagacgttgc gtgagtgaac cagagttccc cgcgccagcg gggataaacc gaaatatcca
      481 gggctgggct ggaggcagac ggcgagttcc ccgcgccagc ggggataaac caccatataa
      541 cccgttatct ctttctcaag tttttatatt agctgtactt gtaataagca acatatccac
      601 gtaacacctc
//
LOCUS       DBANUU010000482          600 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.489, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000482 DBANUU010000000
VERSION     DBANUU010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.489"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            18..569
                     /locus_tag="ACIE68_005168"
     CDS             18..569
                     /locus_tag="ACIE68_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFX5911283.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
BASE COUNT          179 a          131 c          134 g          156 t
ORIGIN      
        1 ttatataaag gaatattatg tctgctttta aaaagtcact tcttgttgct ggcgtagcaa
       61 tgattctttc caataacgta tttgctgacg aaggtcacgg cattgttaaa tttaaaggtg
      121 aagttatctc tgctccatgt tcaattaaac caggtgacga agacttaact gtaaatctgg
      181 gtgaagtagc agataccgtt ctgaaaagcg accagaaatc tttggctgag cctttcacta
      241 ttcacctgca agactgcatg ttgagccagg gtggaaccac ttattcaaaa gcgaaggtta
      301 ctttcaccac agcgaatact atgacgggtc agactgacct gctgaaaaac accaaagaaa
      361 ctgaaatcgg tggtgccaca ggcgttggcg tacgtattct ggatagccag agcggcgaag
      421 tgactttagg tactccggta gtaattacat tcaacaatac taacagctat caggaactga
      481 acttcaaagc tcgtatggaa tccccaagca aagatgctac cccgggcaac gtttacgctc
      541 aggctgacta taagattgct tacgagtaat cacattaggc cgggttcgcc cggccacatc
//
LOCUS       DBANUU010000483          590 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.490, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000483 DBANUU010000000
VERSION     DBANUU010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.490"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            73..>590
                     /locus_tag="ACIE68_005169"
     CDS             73..>590
                     /locus_tag="ACIE68_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFX5911284.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVT"
BASE COUNT          173 a          115 c          139 g          163 t
ORIGIN      
        1 attattgtta ttattaaggc taatgtcatt taaaggcatt atcatattta cgaagagatc
       61 aggatgatga cgatgaaaaa aagtgtattg acggcgttta taactgtggt atgtgcaacg
      121 tccagcgtta tggcagctga tgataatgct atcacggatg gcaaagtaac atttaatggt
      181 aaagttattg ctccagcttg taccctggta gctgcgacga aagattcagt agtcacattg
      241 ccaaatgtca gcgccaccaa gttgcaaact aatggagcag tctctggcgt taaaactgat
      301 gtaccaattg ccttagaagg ctgtgatgtt actgtaacta aaaatgcgac gtttaccttt
      361 agtggtacgg ctgacggcgt gcaacctaca gcatttgcca accaggctac caccgatgca
      421 gcgacgaatg ttgctctaca aatgtatctg ccagatggtt ctacatcggt aactcccggt
      481 accgaaacgt cgaatattca gttagcggat tctgctgagc aaacagtgac ctttaaagtt
      541 gattatatcg ctacggggaa agcgacttct ggtaatgtga atgcggtaac
//
LOCUS       DBANUU010000484          583 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.491, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000484 DBANUU010000000
VERSION     DBANUU010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.491"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
BASE COUNT          180 a          157 c          109 g          137 t
ORIGIN      
        1 ttaaaaaaca tacagattta ctctgaaatt atttacccag gcctccctga aaagaccatc
       61 cggagcatcc cgttcgtcag cttccatgtt gtcttttacc ccggaaagtg atcgcatttt
      121 ccagccagaa tgacagccat actgattatg acgtctctcc ggccccttca catataacat
      181 tcatgtcagt catttcttac tgcctcactc accggaattc tccatgccgg atgaagcaat
      241 tgttctgaac tgagcagaag cataacagca cacaaggaaa cgactgtgct atagatcctg
      301 gaatcaggat cttaatgtca ggaaaacaat gacgaccggg gataaccaac aacaacctga
      361 gtacagaaga aagctttccc gcgcacgcca tcatcttatg taagaggata tgatgccacc
      421 cccacctcag aagaaccgtc tccgggagaa cttcactgca tccaaaatcc ttttgctgag
      481 ttctcattcc ccatcatggc agctacagac atgccctgac agagcgtatg cgcattgaga
      541 gaaataacca taaatgatga gaagacactc tatataacga cag
//
LOCUS       DBANUU010000485          582 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.492, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000485 DBANUU010000000
VERSION     DBANUU010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.492"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(30..>582)
                     /locus_tag="ACIE68_005170"
     CDS             complement(30..>582)
                     /locus_tag="ACIE68_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFX5911285.1"
                     /translation="GRYITQDPIGLKGGWNFYQYPLNPISNIDPLGLETLKCIKPLHS
                     MGGTGERSGPDIWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTK
                     EAAGRCDLVETDNSCVENCLKGKFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDSLE
                     TCKMKCSGNNIGRLIRFVFTGVM"
BASE COUNT          164 a          147 c           90 g          181 t
ORIGIN      
        1 gctctgaata aaaaaaataa tatatttcat tacatcactc cggtgaatac aaatctaata
       61 agacgtccaa tgttatttcc ggagcacttc atcttacatg tttctaaaga atcattagac
      121 cagtcttggc agtttttaaa tagccctaaa tttataggtg tgaatatatc aggcaataca
      181 gaataacgcg gccttacctc tttaaacttc ccttttaaac agttctccac acaactatta
      241 tcggtttcaa cgaggtcaca acggccagca gcttcttttg tatcattact tgctttacct
      301 ggcccccata gaccatctcc ttttgattct ccccgttggt cttggccacc acaagtatag
      361 tcccctttac catctgggac acaaagatat tgatgataga acggattccc ccatatatct
      421 ggaccgcttc tttcaccagt tccgcccatt gaatgcagtg gcttaatgca ttttagtgtt
      481 tctaatccta atggatctat atttgagatc ggattcaacg gatactgata aaaattccat
      541 ccccccttca gcccaatcgg atcctgagtg atataccgcc cc
//
LOCUS       DBANUU010000486          569 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.493, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000486 DBANUU010000000
VERSION     DBANUU010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.493"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            73..>569
                     /locus_tag="ACIE68_005171"
     CDS             73..>569
                     /locus_tag="ACIE68_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin"
                     /protein_id="HFX5911286.1"
                     /translation="MKFVSERIDHVKIANGYCVICGNFGKLKKDHVPPQCASKPSPML
                     QKTISEYFSNTRVPPIKAKSGTTFRTICHNCNSNILGSMDAEIGRVTTEFIEKLNDYF
                     SGKFFYKNYISLKFDADKFLRAMIGHLLAATSVKDCMKPIVDSPFYTPLRNFVLGRNP
                     DIGAT"
BASE COUNT          194 a           84 c          101 g          190 t
ORIGIN      
        1 agagtaaaat catattgaac attgtgtttg aatgctctat attttggtga tgttaacttt
       61 tgagattgca gaatgaaatt tgtctctgaa agaattgacc atgtaaaaat agctaatgga
      121 tattgtgtaa tatgtggtaa ttttggaaaa ttaaaaaaag accatgttcc cccgcaatgt
      181 gcatctaaac cttcacctat gttacaaaaa acgatttctg agtatttctc taatactcga
      241 gtaccaccaa ttaaagcaaa aagcgggaca acttttcgta ccatttgtca caattgtaac
      301 tcgaatattt taggatcaat ggatgcagaa ataggccgtg ttacaactga attcatcgaa
      361 aagttaaatg attattttag tggtaagttc ttttacaaaa attatatttc attaaaattt
      421 gatgcggata aatttctaag agcaatgatt ggacaccttc tggctgcaac ttcagtaaaa
      481 gattgtatga agcctattgt tgatagtcct ttttatactc cattgagaaa ttttgtatta
      541 ggcaggaatc cagacattgg ggcaactca
//
LOCUS       DBANUU010000487          563 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.494, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000487 DBANUU010000000
VERSION     DBANUU010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.494"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>563
                     /locus_tag="ACIE68_005172"
     CDS             <1..>563
                     /locus_tag="ACIE68_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1380-like element ISEcp1 family transposase"
                     /protein_id="HFX5911287.1"
                     /translation="NGIFDFIENDLVFDNDSTNKIKMNHIKTMLCGHFIGIDKLERLK
                     LLQNDPLVNEFDISVKEPETVSRFLGNFNFKTTQMFRDINFKVFKKLLTKSKLTSITI
                     DIDSSVINVEGHQEGASKGYNPKKLGNRCYNIQFAFCDELKAYVTGFVRSGNTYTANG
                     AAEMIKEIVANIKSDDLEILFRMDSGY"
BASE COUNT          205 a           93 c          102 g          163 t
ORIGIN      
        1 gcaatgggat ttttgatttt attgaaaatg acctcgtatt tgataatgac tcaacaaata
       61 aaatcaagat gaatcatata aagaccatgc tctgcggtca cttcattggc attgataagt
      121 tagaacgtct aaagctactt caaaatgatc ccctcgtcaa cgagtttgat atttccgtaa
      181 aagaacctga aacagtgtca cggtttctag gaaacttcaa cttcaagaca acccaaatgt
      241 ttagagacat taattttaaa gtctttaaaa aactgctcac taaaagtaaa ttgacatcca
      301 ttacgattga tattgatagt agtgtaatta acgtagaagg tcatcaagaa ggtgcgtcaa
      361 aaggatataa tcctaagaaa ctgggaaacc gatgctacaa tatccaattt gcattttgcg
      421 acgaattaaa agcatatgtt accggatttg taagaagtgg caatacttac actgcaaacg
      481 gtgctgcgga aatgatcaaa gaaattgttg ctaacatcaa atcagacgat ttagaaattt
      541 tatttcgaat ggatagtggc tac
//
LOCUS       DBANUU010000488          559 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.495, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000488 DBANUU010000000
VERSION     DBANUU010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.495"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(52..>559)
                     /locus_tag="ACIE68_005173"
     CDS             complement(52..>559)
                     /locus_tag="ACIE68_005173"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911288.1"
                     /translation="MNNFAKFIISSTIGFFSLSAIAATGPSINSKTLSITLHSPANIT
                     ANWVAESDLTPAKIMQPETLVGVISLSNTSIETATIRSTKPADASQQGVITFENGSGD
                     TFGTTISTNNSNVSIEKRTGESVISSTTQGAFLPSNLDLNVKTTSGNTTVSSGQYNAV
                     VSITTQIK"
BASE COUNT          163 a           94 c          116 g          186 t
ORIGIN      
        1 ctatcgccac tatggagttt agcctcctct aaaaaaggag gcttgagaag attatttaat
       61 ctgcgtggta attgatacta cagcattata ttgtccagac gatacagttg tattgccaga
      121 tgttgttttt acattaagat ctaaatttga tggtaaaaat gcgccctgag tggttgagct
      181 aataacactt tctccagttc tcttttcaat ggatacattg gagttatttg ttgatatggt
      241 agtgccaaat gtgtcaccgc taccattctc aaaagtaata acaccttgct ggctagcatc
      301 agcaggcttg gtgcttcgaa tagttgctgt ttcaatactg gtattggata ggctaataac
      361 accaaccaac gtttctggct gcattatttt agcaggagtt aaatctgact ctgcaaccca
      421 gtttgcagta atattggctg gagagtgaag agttatactt aatgtttttg aattaattga
      481 aggtcctgta gctgcaatag cacttaagga aaagaatcca attgttgagc taataatgaa
      541 ttttgcaaag ttattcatg
//
LOCUS       DBANUU010000489          540 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.496, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000489 DBANUU010000000
VERSION     DBANUU010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.496"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..194)
                     /locus_tag="ACIE68_005174"
     CDS             complement(<1..194)
                     /locus_tag="ACIE68_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFX5911289.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVV"
     gene            complement(206..>540)
                     /locus_tag="ACIE68_005175"
     CDS             complement(206..>540)
                     /locus_tag="ACIE68_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911290.1"
                     /translation="NSNNMAIYWNSRASLFCSTELNSKSQSPALGLGHEFAHAQYYLL
                     DKENFMALLSRTDKKYENKEEARVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQT
                     MKITGTPE"
BASE COUNT          166 a          135 c           72 g          167 t
ORIGIN      
        1 ttcaccacca ctttatatct gtatatttca ttaagacctt ccttgctaag cggaactaac
       61 gtggtaacat acgatggaag attaattatc tctatcgttt tcacgctatc tattccacac
      121 aattcattca ctcgccccat ccccagtacc gaaccagaaa atagagacaa taaaaccatc
      181 aaatatttca tcataaaaaa cctcactatt ctggcgtacc cgttattttc atcgtctgca
      241 acggtccatc cacccgataa aaaggcagtc cactatgagc tcctctggta cactcaccta
      301 aagttttcgc cgcacgcgat tcaattattg taataaccct tgcctcttct ttattctcat
      361 atttcttatc tgttcggctt aaaagagcca tgaaattttc cttatcaaga aggtaatact
      421 gcgcatgagc aaactcatgt cccaaaccta atgcaggcga ttgtgatttc gagtttaatt
      481 cagttgaaca aaataaagat gctctactat tccaatatat cgccatatta ttcgaattaa
//
LOCUS       DBANUU010000490          532 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.497, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000490 DBANUU010000000
VERSION     DBANUU010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.497"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..340
                     /locus_tag="ACIE68_005176"
     CDS             <1..340
                     /locus_tag="ACIE68_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFX5911291.1"
                     /translation="AVRIRLVNIDAPEKKQDYGRWSTDMMRSLVAGKTVTVTYFQRDR
                     YGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADP
                     VPPWIWRHPK"
     gene            386..>532
                     /locus_tag="ACIE68_005177"
     CDS             386..>532
                     /locus_tag="ACIE68_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFX5911292.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLA"
BASE COUNT          166 a          108 c          134 g          124 t
ORIGIN      
        1 ggcggtgcgg atcaggctgg taaatattga tgcaccggaa aagaaacagg attatggccg
       61 gtggtcaaca gacatgatga gatcactggt cgcaggaaaa acggttaccg tcacatattt
      121 tcagcgagat cgttatggtc gtattctcgg gcaggtttat gcaccagacg gaatgaatat
      181 taatcagttt atggttcggg ccggggctgc ctgggtatat gagcagtaca acacagatcc
      241 cgtgctgcct gtccttcaga acgaagccag acaacaaaaa cgtgggctat ggtcagatgc
      301 tgatccggtt ccaccctgga tatggagaca ccctaaataa agcgatggca atcttatcat
      361 tcatctgttc tcagcgggaa taaaaatggc aaaaacaaaa caggaatggc tgtatcagtt
      421 acggcgatgt tcatcagtca atacgctgga aaaaattatt cataaaaaca gagacagttt
      481 attaaacagt gaacgtgaat catttaattc agcagcagac caccggttag cg
//
LOCUS       DBANUU010000491          530 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.498, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000491 DBANUU010000000
VERSION     DBANUU010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.498"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>530
                     /locus_tag="ACIE68_005178"
     CDS             <1..>530
                     /locus_tag="ACIE68_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFX5911293.1"
                     /translation="HADGSVTQSQFDAVGRLKAQTDAAGRTTEYSPDVVTGLITRITT
                     PDGRASAFYYNHHSQLTSATGPDGLEMRRKYDEYGRLIQETAPDGDITRYRYDNPHSD
                     LPCATDDATGSRKTMTWSRYGQLLTFTDCSGYVTRYDHDRFGQVTAVHREEGLSQYHA
                     YDSRGQLTAVKDTQGH"
BASE COUNT          129 a          155 c          157 g           89 t
ORIGIN      
        1 aacacgcgga cggcagcgtc acgcagagtc agtttgatgc ggtgggcagg cttaaggcac
       61 agacggatgc cgcaggcagg acaacagagt acagcccgga tgtggtgacg ggactcatca
      121 cgcgcatcac cacgccggat ggcagggcat cggcgtttta ctataaccac cacagccagt
      181 taacgtcagc caccgggcct gacgggctgg aaatgcgccg caaatatgat gagtacggac
      241 gtctgattca ggaaaccgcc cctgacggcg atatcacccg ctaccgttat gataatccac
      301 acagtgacct cccctgcgca acggacgatg ccaccggcag ccggaaaacc atgacgtgga
      361 gccgttacgg tcagttgctg accttcactg actgttccgg ttatgtaacc cgctatgacc
      421 atgaccgttt tggtcaggtg acggcggtgc accgcgagga aggcctgagc cagtaccacg
      481 catacgacag ccgtggacag ttaactgcgg tgaaagacac gcagggccat
//
LOCUS       DBANUU010000492          526 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.499, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000492 DBANUU010000000
VERSION     DBANUU010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.499"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>526
                     /locus_tag="ACIE68_005179"
     CDS             <1..>526
                     /locus_tag="ACIE68_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFX5911294.1"
                     /translation="FAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSPGSLPFTTA
                     ATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIY
                     LKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQKWLKLAAK
                     QHYKNAERLLAGLPA"
BASE COUNT          162 a          118 c          116 g          130 t
ORIGIN      
        1 attcgcaatt tttatagttg cagcaattac ctgtattagc caacccaaga aaacgacgtt
       61 gcgtgataaa gccatggtga attatgcctt tgattattta agatcaccgg gcagtcttcc
      121 attcaccacg gcagccacgg agctttccgc gattcatggt cactcaacgt cgcaatatcg
      181 ccttggagaa ttttatcttc atggtagcga cggtaaacca ctggattata cacaggcgag
      241 atactggtat gagcaatcag cggaacagga aaatccacgc gcgcaaagta aactggggtg
      301 gatctacctc aaaggtctgg gggtcaaacc cgacacccgt aaagcaattc tctggtataa
      361 ggaagcagct gaacaagggt atgctcatgc tcaatatact ttaggtttga tctacagaaa
      421 tggctcaggt attaatgtta accattatga atctcaaaaa tggttaaaac tggccgccaa
      481 acaacattac aaaaatgcgg aaagattact tgccgggctt cccgca
//
LOCUS       DBANUU010000493          523 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.500, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000493 DBANUU010000000
VERSION     DBANUU010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.500"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(146..>523)
                     /locus_tag="ACIE68_005180"
     CDS             complement(146..>523)
                     /locus_tag="ACIE68_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFX5911295.1"
                     /translation="RSLLQSQQNSDEALNIKRDADPTFDFIGYLETLPQTSGMYMGNA
                     SIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEYGLNYEKRHTKQGIQ
                     TNLTLKEESYGDWLPKCDDPATT"
BASE COUNT          109 a          124 c          141 g          149 t
ORIGIN      
        1 cactgttcac ctttcgtttt tctcttttaa tttcagtgtg ataacgggtg aatatacggt
       61 gaagggtgaa cagtggattg ttcaccttcg aggaattcag ggataaaaaa agaccggcag
      121 atgccggtca gatgagtcat gatggtcagg ttgttgcagg gtcgtcacat tttggcagcc
      181 agtcgccgta gctttcctct ttcagcgtca ggttggtctg tatcccctgt ttggtatggc
      241 gcttctcgta attcagtccg tattccttca gcatcaccgg cagccccagc ccgaacattt
      301 tcagactgag tacattccgg tagccgtttg cctccatgta ggccagatag gcgtgataga
      361 ggtatttacg gtaattacgc gggatgatac tggcgttccc catatacatg ccgctggtct
      421 gcggcagggt ttccagatag ccgataaaat caaacgtcgg gtcggcatcc cgtttgatgt
      481 tcagcgcctc gtctgagttc tgctgggact gaagcagtga ccg
//
LOCUS       DBANUU010000494          523 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.501, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000494 DBANUU010000000
VERSION     DBANUU010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.501"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            22..522
                     /locus_tag="ACIE68_005181"
     CDS             22..522
                     /locus_tag="ACIE68_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFX5911296.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLNPNTEPVNILLSNNFIRINKNITLSDLTHFLISNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTISYHKIRALNKLNIKGTVELFIALCEWDKHYFKLQ
                     SCVRES"
BASE COUNT          180 a           92 c           82 g          169 t
ORIGIN      
        1 ttttagagta caggttggca tatgcaagtc ttttccagtg atgtctactt cactgtaggc
       61 actaatgcat tattagcatc ccaaaaagaa tattacagcg atttagttgc gttggttgat
      121 cttgggcatt cgttcgttgt tattgatgag catcaacatc gaaatctcaa tccaaataca
      181 gaacctgtaa acatcctttt aagcaacaat ttcatcagga tcaataagaa tattacatta
      241 tctgatttaa cgcatttcct gatttcgaat cttcatacac aaaatgtgta ttccacacaa
      301 gaaccactta cccatgatga gatagatatc ctgcggttat gtgtttctta ctcgttaaaa
      361 caaatagcca ttattaaagg aattgattat aaaacaatat catatcacaa aataagagcc
      421 ctaaataaac ttaacataaa aggtactgtt gagttgttta tagcactctg tgaatgggat
      481 aaacattatt ttaaactcca atcttgcgta agagaaagtt gat
//
LOCUS       DBANUU010000495          521 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.502, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000495 DBANUU010000000
VERSION     DBANUU010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.502"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(148..>521)
                     /locus_tag="ACIE68_005182"
     CDS             complement(148..>521)
                     /locus_tag="ACIE68_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFX5911297.1"
                     /translation="RYIRSHEAVQHISIRDRLNDFMQQHGTVLAAALAPELMGYSELT
                     VIARNCAIQRATDALREALLSWLAKVEKINYSAQDSDILTTIGFRPDAASVDDSREKF
                     TPAQNMIFSRKSAQLASHQSV"
BASE COUNT          125 a          127 c          142 g          127 t
ORIGIN      
        1 ggttttaatg cagttgcact gacacgctca ggccgcgccg ggaatggcgc ggtttgcaga
       61 aaatgaggca ggaaaacgca tgcaaagcca tgcaccttat cgatgcatgg cttttttcag
      121 taaaaacggg cggattttcg gggagtttta cacagactga tgtgatgcca gttgcgcact
      181 tttacgcgaa aaaatcatgt tctgcgcagg agtgaatttt tcacggctgt catccaccga
      241 agccgcgtca ggcctgaatc cgatggtcgt taaaatgtcg ctatcctgtg cggaataatt
      301 aattttttca acctttgcaa gccaggacaa aagggcttca cgcagggcat ctgtggcacg
      361 ctgtatggca cagtttcggg caatgaccgt cagttcactg tagcccatca gctccggtgc
      421 cagtgcggcc gccagtactg tgccgtgctg ctgcataaaa tcattcagcc ggtcgcggat
      481 gctgatgtgc tgaacggctt catgcgaacg aatataacga c
//
LOCUS       DBANUU010000496          518 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.503, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000496 DBANUU010000000
VERSION     DBANUU010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.503"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>518
                     /locus_tag="ACIE68_005183"
     CDS             <1..>518
                     /locus_tag="ACIE68_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911298.1"
                     /translation="ILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLELAIGWRGALS
                     ESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGSLAS
                     FACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDGLKRQQELDKW
                     KRENMTPFMYVL"
BASE COUNT          155 a           85 c          116 g          162 t
ORIGIN      
        1 aaatacttgg agagaaatat ataaaaatca caacatccag cccctggctt ttagaaaagc
       61 tcggtaaata tattttctct tcacgagcgc ctcaagttct ggaactcgct attggctggc
      121 gaggagcttt atctgaatca ataaaaggag ttaagttctg tatctggttt tcagtagcct
      181 ggagaaccat agaatttatt atgagttcag agcgagatct ggttaatttt cttggcgatt
      241 tttcaatgga tgtggccaaa gcagttatag ccggtggtgt tgcaaccgct attggaagcc
      301 tggcttcttt tgcctgtgtt agctttggct ttccagtaat tcttgtcgga ggagcaattt
      361 tactgacagg gatagtgtgt acagttgttt taaatgaaat cgatgctcaa tgccatttat
      421 cagaaaaatt aaaatatgca attagagatg gactaaaacg gcaacaggaa cttgataaat
      481 ggaaaaggga aaacatgact ccatttatgt atgttctt
//
LOCUS       DBANUU010000497          501 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.505, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000497 DBANUU010000000
VERSION     DBANUU010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.505"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..279
                     /locus_tag="ACIE68_005184"
     CDS             <1..279
                     /locus_tag="ACIE68_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911299.1"
                     /translation="GSFTHYGEELQGPTVDGNELREETRYLNVDYAAVTGLLVQVARE
                     TDDRVTALEEENTTLRENLATAGTRITTLENQVSELVALVRQLTGSEH"
     gene            316..459
                     /locus_tag="ACIE68_005185"
                     /pseudo
     CDS             316..459
                     /locus_tag="ACIE68_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          145 a          102 c          132 g          122 t
ORIGIN      
        1 ggatcgttta ctcattacgg tgaagagttg cagggaccaa cggttgacgg caatgaatta
       61 cgtgaagaaa cacgttatct gaatgttgac tacgccgccg tgacggggtt acttgttcag
      121 gtcgcccgtg aaacagatga tcgcgttacc gcgctggaag aggaaaatac aacgttacgt
      181 gaaaatctgg caacagcagg cacccggatc accactctgg aaaatcaggt aagcgaactg
      241 gttgcacttg tccggcagtt aacaggaagc gaacattgat atccttcaag ccctgaagta
      301 ggctgttccc ggtacgttca gactgttgtt gagctggaga tcgcaacgga ggaagaaatc
      361 tcgttgctgg aagcatggaa aaagtatcgg gtattgctga accgtgttga tacatcaact
      421 gcaccggata ttgaatggcc agtagcacct atagggtaaa ttcgtaatga ttacctaaat
      481 acgttattct tttgttaaaa a
//
LOCUS       DBANUU010000498          501 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.506, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000498 DBANUU010000000
VERSION     DBANUU010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.506"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..135
                     /locus_tag="ACIE68_005186"
     CDS             <1..135
                     /locus_tag="ACIE68_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFX5911300.1"
                     /translation="VTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            179..>501
                     /locus_tag="ACIE68_005187"
     CDS             179..>501
                     /locus_tag="ACIE68_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911301.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMT
                     EFDDNG"
BASE COUNT          165 a          100 c          101 g          135 t
ORIGIN      
        1 gtcacaccgc atgctgtcct ggaaaaagaa ctagaaccac aggagagaat tgcctttatt
       61 tatgaagata tttcctggga gcatacgtta gccggtacga atgcaatgag taaatggcag
      121 gatcgtgtac agtaacttac tgtaataaca taataaatta ctattgcgga gagataaaat
      181 ggctggttta aggaataaca acaacacaca gaatgcacag tgggctgatt atgtgggtga
      241 cattcttcgg ggggctcagc cgataaacca gctagtacct cagcaccctt atcttaacga
      301 cgtcccactc attgatgagt taaggcatca gaacactcat catgttattc tcttgacact
      361 tgatgttgca aaaaaaatac tcagcccaat aacatcattt gattacattc attttatcac
      421 tacacatcct tcaggtataa aagatacact cgcctggtta gttaacgcga gtaaattaat
      481 gactgagttt gatgacaatg g
//
LOCUS       DBANUU010000499          500 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.507, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000499 DBANUU010000000
VERSION     DBANUU010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.507"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
BASE COUNT          166 a          101 c           81 g          152 t
ORIGIN      
        1 aacacttact gaaatgctta gtaaaatacg tgtatatagt atcataccga cattctcaac
       61 atcttcagac atccatgcag actgagtgtt gatttcagtt tttcacggcc agcatggatc
      121 tttactggtc aaaaaaacag acaggtttac tctggagtta ttttcctgtt cacgtattca
      181 gtacagcaga aatcccgtga taacgagaca gtaaaccatc gtaatcagcg tctgtaactc
      241 catcgcttta ccccagaaaa gttaagccat ataatgtgat ggatataagt cgtcgtatcc
      301 ggtaagtaca gataaccaca acataagctc attcagtaaa ttttatctct gaacaaacga
      361 ctatggcatg ctcatttata ctattcataa gaaagtgtga ttatctgtaa gcattaacca
      421 tcaaatcata taaccatact aaactggcgg atcatcagca ccattagcag gtaacttatt
      481 gaaattttat tatgtgtttt
//
LOCUS       DBANUU010000500          488 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.508, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000500 DBANUU010000000
VERSION     DBANUU010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.508"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>488)
                     /locus_tag="ACIE68_005188"
     CDS             complement(<1..>488)
                     /locus_tag="ACIE68_005188"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911302.1"
                     /translation="IGLLFVMVSGANAATGPSVNNQRLDVSVTSTPVLNVSFEPNQNL
                     YAGTNYGISVAADAGILTFNTSGLTDITIVSGRKAVDRQGGNNFAFFHNASGNYRLRS
                     SLVTTSNNVSLSDIGLQGVKIAPINGSPLLPDSFAFRMKVNTNNGNGVNLPAGEYTSI
                     IN"
BASE COUNT          162 a           99 c           87 g          140 t
ORIGIN      
        1 attgatgatt gatgtatact cacctgcagg tagattcaca ccattaccat tattagtatt
       61 gaccttcatt ctaaatgcaa aagaatcggg gagcaaagga ctaccgttta taggagctat
      121 tttaactccc tgaagaccaa tatcacttaa agaaacgttg ttactagtag ttaccaaaga
      181 ggaacgaagt cgatagttcc ctgatgcgtt atgaaaaaat gcaaaattat tccctccctg
      241 tctgtcgaca gcttttcgtc cagaaacaat ggtaatatca gttaatccag aggtattaaa
      301 agtcaagatt ccagcatcag ctgctacaga aataccatag ttagttcctg cgtataaatt
      361 ttgatttggt tcaaaactaa cattgagcac aggtgtgctt gttactgata catcaagcct
      421 ctgattattt acactaggac cagtagcagc attagcaccc gacaccataa cgaataagag
      481 tcctatta
//
LOCUS       DBANUU010000501          486 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.509, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000501 DBANUU010000000
VERSION     DBANUU010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.509"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>486
                     /locus_tag="ACIE68_005189"
     rRNA            <1..>486
                     /locus_tag="ACIE68_005189"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          121 a          110 c          163 g           92 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaacct
//
LOCUS       DBANUU010000502          479 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.510, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000502 DBANUU010000000
VERSION     DBANUU010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.510"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>479)
                     /locus_tag="ACIE68_005190"
     CDS             complement(<1..>479)
                     /locus_tag="ACIE68_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFX5911303.1"
                     /translation="FTLLSGNFKPDWRWDVSKETFTKEKFDSYVKPVFSKIDFYKQCG
                     VINPQNANTAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHEEMASSAYKH
                     KNNKVLYDLLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTL"
BASE COUNT          156 a          100 c           74 g          149 t
ORIGIN      
        1 caaagtaagg atatatttat caccaaaaat agtactaaat ctatcgcctg tatgatcccc
       61 aaggaagata caaggggtta tcgcatcgtc ctttaaaaaa acatgttgat gcaactcatt
      121 caagatcatg tctctcgtat ttaataaatc gtagagcact ttattatttt tatgtttgta
      181 tgcagatgaa gccatctctt cgtgttttaa taattcacat aataaactcc aacccttttc
      241 cctgatgcct atatgccctg aaacgagcaa tgcataaagt acagcgccta ctcttccatc
      301 tgtatcgcca aaataagccg tgttggcatt ttgtgggtta atcacaccac actgtttata
      361 gaagtctatt tttgaaaaca caggtttaac atagctatca aacttttcct tagtaaacgt
      421 ctctttagag acatcccaac gccagtcagg tttgaaatta ccactaagaa gagtaaaga
//
LOCUS       DBANUU010000503          474 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.511, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000503 DBANUU010000000
VERSION     DBANUU010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.511"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..144)
                     /locus_tag="ACIE68_005191"
     CDS             complement(<1..144)
                     /locus_tag="ACIE68_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911304.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIM"
     gene            162..353
                     /gene="yfgG"
                     /locus_tag="ACIE68_005192"
     CDS             162..353
                     /gene="yfgG"
                     /locus_tag="ACIE68_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFX5911305.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          127 a          119 c           80 g          148 t
ORIGIN      
        1 cattattaca tcatcattgt aaataattaa attaacttcc ataacattaa aatatgtatc
       61 cactgacgct tttttacata acgaagaatt gaccattttg tcctgttgtg ccttaatgta
      121 agtaccgtcc acagcgtggg acatacttca aggaaccttt tgtgagtcag gcaaccagta
      181 tgcgaaaacg acaccgattt aacagtcgca tgacccgtat cgtactgctc atcagcttta
      241 tcttcttctt tggccgtttt atctactcgt ccgtcggtgc ctggcagcac catcagagca
      301 aaaaagaagc tcagcaatcc acactctccg tcgaatcacc ggtacaacgt tagcggttac
      361 cttctccact ttcacagaac ctaacggcac ttcgctgtcg gatgcttttg ctctttggaa
      421 ttatcaaagc ggcagatatt ctttcatctt aaattttacg tctttgtcct gact
//
LOCUS       DBANUU010000504          469 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.512, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000504 DBANUU010000000
VERSION     DBANUU010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.512"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..307
                     /gene="traP"
                     /locus_tag="ACIE68_005193"
     CDS             <1..307
                     /gene="traP"
                     /locus_tag="ACIE68_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFX5911306.1"
                     /translation="ICHYRAADAADYVRETDRSLMQLVTALWATLALMYVSLAAITGK
                     YPVRPGKMKCIRVVTADEHLKEVYTEDASLPGKIRKCPVYLPDDRTNRNNGDKNEHA"
     gene            246..>469
                     /locus_tag="ACIE68_005194"
     CDS             246..>469
                     /locus_tag="ACIE68_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFX5911307.1"
                     /translation="MFIFLMTEQTETTEIKMNMRNINVITALSVPGKTVSDDFMHAVL
                     SNCATRIVLPAPEKFGSESLPDNFNMTAVGV"
BASE COUNT          131 a          106 c          116 g          116 t
ORIGIN      
        1 tatctgccat taccgcgcgg ctgatgctgc agattatgtc agggagactg accggtcact
       61 gatgcaactg gtcaccgcat tatgggcaac actggcactg atgtatgtct cgctggcagc
      121 gatcaccggg aaatatccgg tgcgacccgg aaaaatgaaa tgtatccggg tggtaaccgc
      181 agatgaacat ctgaaggaag tttatacgga agacgcttcc ctgccgggaa aaatcaggaa
      241 atgccctgtt tatcttcctg atgacagaac aaacagaaac aacggagata aaaatgaaca
      301 tgcgtaatat taatgttatt acagccctct ctgtgccggg taaaaccgtg tcagatgatt
      361 ttatgcatgc tgttctcagt aactgcgcca caagaatcgt acttcctgca ccggagaaat
      421 tcggttctga gtcattgccg gacaatttta atatgactgc tgtcggtgt
//
LOCUS       DBANUU010000505          468 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.513, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000505 DBANUU010000000
VERSION     DBANUU010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.513"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>468)
                     /locus_tag="ACIE68_005195"
     CDS             complement(<1..>468)
                     /locus_tag="ACIE68_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HFX5911308.1"
                     /translation="AALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVL
                     PAPLPPYRVLTGLVDRFGRTQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAE
                     EARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPL"
BASE COUNT           77 a          162 c          150 g           79 t
ORIGIN      
        1 agcggcgcgg caggtaaatt ctccgggtat tccgggtcgt gcgtcagcca cacggcagac
       61 agacggatgc cgttgtcccg gccatattcg gtgtaacccg gcagggtatc aggaaaagcg
      121 gacggttccg tcccgccgga aatggcctgc tgccgggctt cttctgcccg ctgcgcctgc
      181 gtggtcagta ccagccggaa gtgacgcccg gcaccatccg tcacgccggt gatttcgccg
      241 ctgaattcac cggcggcttc gcggtggaac gtctgtgtgc gcccgaagcg gtccaccagc
      301 ccggtcagta cccggtacgg cggcagcggc gcaggcagca cctcatccgc ttccggcacc
      361 cgctcacacc agccgagcag ccaccacggc ccctgcggac tgtttgtcgc cagataacga
      421 tgcggactta agcggagttc ttccggcagc gcctgccaga gtgcggcc
//
LOCUS       DBANUU010000506          464 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.514, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000506 DBANUU010000000
VERSION     DBANUU010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.514"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>464
                     /locus_tag="ACIE68_005196"
     CDS             <1..>464
                     /locus_tag="ACIE68_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFX5911309.1"
                     /translation="SGDTIAVIEAPGASNARINGLGGGETNYFGYALASYLEPYRENR
                     ISMDPLSLPSDISLDFTSTSVIPTKGAFIPAKFKTNRGKKVLLNLEDEYGNPIPFGAI
                     VRVVSDSDNMSIVGDDGVVYLTAMKDTGVIEVVWGDRNNQKSRKIGYSLNDTP"
BASE COUNT          149 a           77 c          109 g          129 t
ORIGIN      
        1 tctggggata ctattgcagt gatagaagct ccaggagcat caaatgcaag aataaatggt
       61 ttaggtggtg gtgagacaaa ttactttggt tatgcattgg ctagttattt ggaaccatac
      121 cgagaaaata gaatatcaat ggacccgtta tctttaccct ctgacatctc actggatttt
      181 accagtacat cagttattcc gacaaagggg gctttcatac cagcaaaatt caaaacaaat
      241 agaggaaaaa aggttttgct caaccttgaa gatgaatatg gaaaccccat tccatttggt
      301 gcgatcgttc gagtcgttag tgatagtgac aatatgagta ttgtcggtga tgatggagtt
      361 gtatatctaa ctgcaatgaa agacactggc gttattgagg ttgtctgggg agatagaaat
      421 aaccagaaaa gccgaaagat tggctatagc ctgaatgata ctcc
//
LOCUS       DBANUU010000507          463 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.515, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000507 DBANUU010000000
VERSION     DBANUU010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.515"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            6..239
                     /gene="ydfK"
                     /locus_tag="ACIE68_005197"
     CDS             6..239
                     /gene="ydfK"
                     /locus_tag="ACIE68_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFX5911310.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNQNLNGKV"
BASE COUNT          171 a          101 c           66 g          125 t
ORIGIN      
        1 tcttcatgaa atcaaaagac accctaaagt ggttccctgc gcagcttcct gaagtcagaa
       61 ttatcctggg agatgctgtc gtggaagtag caaaacaggg aagacctatc aataccagaa
      121 cattgcttga ttacattgaa ggaaacataa agaaaaagtc atggctggat aacaaagaat
      181 tattacaaac agcgatatca gttcttaaag acaaccaaaa tttaaatggt aaagtgtaat
      241 acccccctga aaaataattt ttcaatgcat taaaaaaata tcaacaacta cgttattccg
      301 caacaaccta ctttttttac catttttcca ctttaacaac attttgctcc acttttccac
      361 gaccaaacaa cttgaaatct ggttaaaata acacgcaaca ctattcttct tccttgagtc
      421 cgcccggaac tcgaaaaaca aaccgagtta aagctatttt tca
//
LOCUS       DBANUU010000508          462 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.516, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000508 DBANUU010000000
VERSION     DBANUU010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.516"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..368)
                     /gene="traY"
                     /locus_tag="ACIE68_005198"
     CDS             complement(<1..368)
                     /gene="traY"
                     /locus_tag="ACIE68_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-bindin protein
                     TraY"
                     /protein_id="HFX5911311.1"
                     /translation="MKRFGTRSATGKMVKLKLPVDVESLLIEASNRSGRSRSFEAVIR
                     LKDHLHRYPKFNRAGNIYGKSLVKYLTMRLDDETNQLLIAAKNRSGWCKTDEAADRVI
                     DHLIKFPDFYNSEIFREADK"
BASE COUNT          130 a          107 c           68 g          157 t
ORIGIN      
        1 tctttatctg cctccctgaa tatctccgag ttataaaaat caggaaactt gatcaaatga
       61 tcaataactc tgtctgcagc ctcatctgtt ttacaccatc cactacgatt tttggctgca
      121 ataagtagct ggttagtttc atcatccaga cgcattgtca gatacttaac cagcgactta
      181 ccatagatat tccctgccct gttaaacttt ggatagcggt gaagatgatc tttaagtctt
      241 attactgcct caaacgatcg acttcttccg cttctgttac ttgcctcaat taatagactt
      301 tccacatcta caggtaattt tagttttacc atcttacctg ttgcagaacg tgtaccaaat
      361 cttttcaata acacctcccg ctgtttatct tctggttacc acttatgttt gcagaataat
      421 cagaaaaggt aaatagacgg aaagtctata ttttattaac ac
//
LOCUS       DBANUU010000509          457 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.517, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000509 DBANUU010000000
VERSION     DBANUU010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.517"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..353)
                     /locus_tag="ACIE68_005199"
     CDS             complement(<1..353)
                     /locus_tag="ACIE68_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFX5911312.1"
                     /translation="MENNDKFLSQELLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTFRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEK"
BASE COUNT          152 a           90 c           65 g          150 t
ORIGIN      
        1 tttttttctt ttacagcaaa tttgagacct aaaacttcta tttgctgatt caatgttaac
       61 gttctatctt caggttcttg tgctgttaat tctctgaatg ttactaccag atcagggtcg
      121 gtagtaatta ttatctcaaa actacctgta agctcgccat aaggaacgac tattgtgttt
      181 tctggtaact cagaaaaagc atcacttctt cctaacgaaa catcattacc aatgataaca
      241 agtagacgcc cattaagtat ttcatactcg catccattca atggtccgct caacaaacga
      301 atggcataag attccaataa ttcttgtgat aagaatttat cattattttc catgaatacc
      361 acccgctaat tccataagct ctagtttaga gtagtgtaac aagacggaaa tatcatgacc
      421 aaattatgat gacacagaat aaaaaaatta taatttc
//
LOCUS       DBANUU010000510          453 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.518, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000510 DBANUU010000000
VERSION     DBANUU010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.518"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
BASE COUNT          133 a           98 c           79 g          143 t
ORIGIN      
        1 atatatgaaa aggctctcgt taagatgtct ggtacaaaat gacagatcat atgccttttg
       61 taaagagacg acagaagttg agtcttacag gcacatcata aataccctcc ggccatgccg
      121 gagggtattt atttaactgc cgctgtttgc actggatgtg gaactactca tactataaga
      181 aggtccactt ccccccggat acatgatgat ggatggagta cccggagccc agcatgctcc
      241 attagcatca atatatgttc ccatatcttt tcctcgtaga tctattacat tagtagaact
      301 aggtaatcca ttaagattca tccctccttt cacgaaatca agcatgtgat ctgatatttc
      361 tctcatttta aaaacctcct gctcatttat gttcagatat taatttggtg ttgcaaccat
      421 ttattatcac agaattcaga atatcattca gtt
//
LOCUS       DBANUU010000511          453 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.519, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000511 DBANUU010000000
VERSION     DBANUU010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.519"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>453
                     /locus_tag="ACIE68_005200"
     CDS             <1..>453
                     /locus_tag="ACIE68_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFX5911313.1"
                     /translation="IWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQD
                     KNAQQQSDTEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTL
                     MAAAKKDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKH"
BASE COUNT          134 a          107 c          142 g           70 t
ORIGIN      
        1 atctggaatc tgcgcaagga tgattatttt gttaacgatg aagcgcgggc gcgttactgg
       61 gatgatcgtg aaaaggcccg tcttgcgctt gaagccgccc gaaagaaggc tgagcagcag
      121 actcaacagg acaaaaatgc gcagcagcag agcgataccg aagcgtcacg gctgaaatat
      181 accgaagagg cgcagaaggc ttacgaacgg ctgcagacgc cgctggagaa atataccgcc
      241 cgtcaggaag aactgaacaa ggcactgaaa gacgggaaaa tcctgcaggc ggattacaac
      301 acgctgatgg cggcggcgaa aaaggattat gaagcgacgc tgaaaaagcc gaaacagtcc
      361 ggcgtgaagg tgtctgcggg cgatcgtcag gaagacagtg ctcatgctgc cctgctgacg
      421 cttcaggcag aactccggac gctggagaag cat
//
LOCUS       DBANUU010000512          449 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.520, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000512 DBANUU010000000
VERSION     DBANUU010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.520"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..367)
                     /gene="traJ"
                     /locus_tag="ACIE68_005201"
     CDS             complement(<1..367)
                     /gene="traJ"
                     /locus_tag="ACIE68_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator
                     TraJ"
                     /protein_id="HFX5911314.1"
                     /translation="MYPMDRIQQKHARQIDLLENLTAVIQDYPNPACIRDETGKFIFC
                     NTLFHESFLTQDQSAEKWLLSQRDFCELISVTEMEAYRNEHTHLNLVEDVFIQNRFWT
                     ISVQSFLNGHRNIILWQFYD"
BASE COUNT          139 a           95 c           79 g          136 t
ORIGIN      
        1 catcataaaa ttgccacaga ataatatttc tgtgtccatt aagaaatgac tggacagata
       61 ttgtccagaa tctgttctga ataaaaacat cctctacaag attaagatgc gtatgctcat
      121 tcctatatgc ttccatctct gtgacagaga tcaattcaca aaaatctctc tgcgacagaa
      181 gccatttttc agcactttga tcttgtgtaa gaaatgactc atgaaacagc gtattgcaaa
      241 aaataaattt tccagtttcg tccctgatac aggctggatt tggataatcc tgaataactg
      301 ccgtcagatt ttccagcaga tctatttgac gagcatgttt ttgttgaata cgatccatcg
      361 gatacatagg aacctcctca caaaggattc tatggacagt cgatgcaggg agttcacgtc
      421 tccctgcatc ggcgattttc aatttcaaa
//
LOCUS       DBANUU010000513          447 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.521, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000513 DBANUU010000000
VERSION     DBANUU010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.521"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>447
                     /locus_tag="ACIE68_005202"
     CDS             <1..>447
                     /locus_tag="ACIE68_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFX5911315.1"
                     /translation="LVKWEKDRRFVVSRVLKPEKERAQLSLLEGSEYDYFFFVTNTTL
                     LSEKVVIYYEKRGNAENYIKEAKYDMAVGHLLLKSFWANEAVFQMMMLSYNLFLLFKF
                     DSLDSSEYRQQIKTFRLKYVFLAAKIIKTARYVIMKLSENYPYKGV"
BASE COUNT          155 a           72 c           86 g          134 t
ORIGIN      
        1 aattagttaa atgggaaaaa gacagaagat ttgtcgtatc tcgcgtactg aaaccagaaa
       61 aagaaagagc acaattatca cttttagaag gttccgaata cgactacttt ttctttgtaa
      121 caaatactac cttgctttct gaaaaagtag ttatatacta tgaaaagcgt ggtaatgctg
      181 aaaactatat caaagaagcc aaatacgaca tggcggtggg tcatctcttg ctaaagtcat
      241 tttgggcgaa tgaagccgtg tttcaaatga tgatgctttc atataaccta tttttgttgt
      301 tcaagtttga ttccttggac tcttcagaat acagacagca aataaagacc tttcgtttga
      361 agtatgtatt tcttgcagca aaaataatca aaaccgcaag atatgtaatc atgaagttgt
      421 cggaaaacta tccgtacaag ggagtgt
//
LOCUS       DBANUU010000514          447 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.522, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000514 DBANUU010000000
VERSION     DBANUU010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.522"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>447
                     /locus_tag="ACIE68_005203"
     CDS             <1..>447
                     /locus_tag="ACIE68_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFX5911316.1"
                     /translation="LVNYSHDNTRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHEL
                     EEKEPRLSGALPYVDSHLHNDLIDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYIL
                     ILLFSLLVVGLSDVILFSKPVPTAVFITIILLCAYFKAQSDQCLLEK"
BASE COUNT          140 a           81 c           80 g          146 t
ORIGIN      
        1 ttagttaatt attcgcatga taacaccaga acttcagtcg gtgcccgtct ggcaatgtat
       61 gaagttggct taaaaacata ttctccaata ggacaatcac tggaaaaacg tgcggaaaaa
      121 atacatgaac tagaagaaaa agagcctaga ttgagtggcg ctttacccta tgtagattct
      181 catttgcata acgatctcat agatacgtta tcaacgcgtg gtattcctgg agttgtatta
      241 acaattttag cattttcagc aatactcata tatgccttaa gaactgctaa agaaccttat
      301 attttaatct tgcttttttc actactggta gtaggactaa gtgatgtaat actcttttct
      361 aaaccggttc cgactgctgt gtttatcacc ataatattgc tttgtgctta ttttaaagca
      421 caatcggacc aatgtttatt agagaag
//
LOCUS       DBANUU010000515          445 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.524, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000515 DBANUU010000000
VERSION     DBANUU010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.524"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..441)
                     /locus_tag="ACIE68_005204"
     CDS             complement(<1..441)
                     /locus_tag="ACIE68_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFX5911317.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAADCIETVNKIKDKNKKIIDINSVNICSDDK"
BASE COUNT          129 a           83 c           70 g          163 t
ORIGIN      
        1 tttatcatca ctacaaatat taactgaatt tatatcaatg atttttttat tcttatcttt
       61 tattttattt actgtttcaa tgcaatcagc ggcaagttgg agtggattta gatttttcag
      121 atatggtctt atgatctttc ccttacttaa taaatagcat gtataggatt cggaaagtcc
      181 agcatctatt ttgtacctca ttatattaga taaggatgtg agaaattcct tgttatcatt
      241 cgcagccata tatgccagtc cgagttcagc ctctgcggcc ttacaggcaa aatagcttag
      301 tgaacacgct tttcgtccct ctttagtact ggctgatatt agtgcccata atattgcttt
      361 gtctgccaaa tctaactctc tctgaaattg tcttgcagta actgcactcg cagaaataga
      421 gaaaaaaata caattgttaa taacc
//
LOCUS       DBANUU010000516          437 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.525, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000516 DBANUU010000000
VERSION     DBANUU010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.525"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            1..437
                     /locus_tag="ACIE68_005205"
                     /pseudo
     CDS             1..437
                     /locus_tag="ACIE68_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
BASE COUNT          158 a           77 c           87 g          115 t
ORIGIN      
        1 caagaagcaa gaaggaaatc cagaaattaa gtctaaacgc agagagcgca tcaggaaatt
       61 ctttctgagc aattgaagtc tgatgtcagt aatagtcgtt tgatgattgc caaccctact
      121 cacattgcaa taggaatata ctttaagcca catctttcac ctattccatt gatttctgta
      181 agagaaacta atgaggtagc cttagccgta aggaaatatg caaaggaaat cggaatacca
      241 attatcacag ataaaaaatt agcacgaaaa atttatgcta cccatcgtcg ctacgattat
      301 gttagcttcg aaaatataga tgaaatatta cgtcttctgc tgtggcttga agatgtggag
      361 aatgctggac aacctgttcc agatgaacag ctctcttcag aagataaatt cattgaaggt
      421 gaagagaaag aaatcga
//
LOCUS       DBANUU010000517          430 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.526, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000517 DBANUU010000000
VERSION     DBANUU010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.526"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
BASE COUNT          134 a           95 c           58 g          143 t
ORIGIN      
        1 atcaatacct aatcagtaca atcttttcct ccatttcttc ttaacatatt tcactctatg
       61 aaagataaaa ctgataccca cctacagttt tgggtcacca acaaaaaaca atgttcgcca
      121 gatattccac cactggcaca gttcaaccct gcttcaaaaa ttgacttatt cattgtgctc
      181 attttcgtag tccgttcagg ttgaataaaa ctcccttcgt caaatgagga aagactgaga
      241 ggttattact ttagcttacg ttatagctgt tttcctttgc agtatttctt tgcttttctg
      301 tataccttta tacctgttat accagatcaa aaaacaagca atccacataa caaaacgcgt
      361 tttgttactg atgtcacaaa ttgagcatat ttttgtagct gatagtttgt tacaaacacc
      421 agaaccaaga
//
LOCUS       DBANUU010000518          430 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.527, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000518 DBANUU010000000
VERSION     DBANUU010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.527"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>430
                     /locus_tag="ACIE68_005206"
     CDS             <1..>430
                     /locus_tag="ACIE68_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFX5911318.1"
                     /translation="SPDGKRSTIEYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNEN
                     GSQSTFRYDPVDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRR
                     TVNGEPAEQWQYDDHGWLTEISHLSEGHRVAVHYGYDDKGR"
BASE COUNT          102 a          120 c          141 g           67 t
ORIGIN      
        1 tatcaccgga cggtaaacgc agcaccatcg aatatgataa acgcgggcgt ccggtgagtg
       61 tgacggaagg cggtctgacg cgcagcatgg ggtatgacgc tgccgggcgc atcactgtgc
      121 tgaccaacga gaacggcagc cagtccacgt tccggtatga cccggtcgac aggctgacgg
      181 agcagcgcgg ctttgacggc cggacgcaac gttaccagta tgacctgacc ggaaaactca
      241 cgcagagtga agatgagggg cttatcaccc tctggcatta cgatgcgtcg gaccgtatca
      301 cccggcgcac ggtgaacggt gaaccggcgg agcagtggca gtatgacgac cacggctggc
      361 tgacagaaat cagccacctg agtgaaggcc accgtgtggc ggtccactac ggctatgacg
      421 ataaaggccg
//
LOCUS       DBANUU010000519          430 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.528, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000519 DBANUU010000000
VERSION     DBANUU010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.528"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(1..>430)
                     /locus_tag="ACIE68_005207"
     CDS             complement(1..>430)
                     /locus_tag="ACIE68_005207"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HFX5911319.1"
                     /translation="HEPDFIVLGYDTELTYSKLVTACRLINCGVDYIATHCDVFCPSE
                     NGPIPDIGTVVTMLEMTTGRKPYRVFGKPNPDLLNLILNEDRLEKDDLLMIGDRIYTD
                     IQMAENTGIDSVLVLTGDTKREDIEDSSVKPTYILQHFSQ"
BASE COUNT          141 a           89 c           70 g          130 t
ORIGIN      
        1 ttactgagag aagtgttgaa gtatataggt cggttttaca gatgaatcct caatatcctc
       61 tcgtttagtg tcacctgtta atacaagaac agaatcaatg cctgtatttt cagccatttg
      121 aatatctgta tatatacggt caccaatcat taacaaatca tctttttcca gccgatcttc
      181 attaagaatc aaattcaaaa gatctggatt tggtttacca aatacgcggt acggcttacg
      241 cccagttgtc atttctagca ttgttactac agtgccaata tcaggaatag gaccgttttc
      301 cgagggacaa aaaacatcac aatgcgttgc aatataatca accccgcaat taattaacct
      361 acaagcggtt accaattttg aataagtcag ctcagtatca tatcctaaca cgataaaatc
      421 aggttcatga
//
LOCUS       DBANUU010000520          422 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.529, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000520 DBANUU010000000
VERSION     DBANUU010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.529"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>422
                     /locus_tag="ACIE68_005208"
     CDS             <1..>422
                     /locus_tag="ACIE68_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFX5911320.1"
                     /translation="AQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSSWSEWEESATDSMSQVKSAATQTFDGIA"
BASE COUNT          116 a          102 c          141 g           63 t
ORIGIN      
        1 gcacaggaga aatccctgct ggcgcataaa gatgagacgc tggagtacaa acgccagttg
       61 gctgcacttg gtgacaaggt tacgtatcag gagcgcctga acgcgctggc gcagcaggcg
      121 gataaattcg cacagcagca acgggcaaaa cgggccgcca ttgatgcgaa aaaccggggg
      181 ctgactgacc ggcaggcagc gcgggaagcc acagaacagc gcctgaagga acagtatggc
      241 gataatcctc tggcgctgaa taacgtcatg tcagagcaga aaaagacctg ggcggctgaa
      301 gaccagcttc gcgggagctg gatggcaggc ctgaagtcca gctggagtga gtgggaagag
      361 agcgccacgg acagtatgtc gcaggttaaa agtgcagcca cgcagacctt tgatggtatt
      421 gc
//
LOCUS       DBANUU010000521          420 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.530, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000521 DBANUU010000000
VERSION     DBANUU010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.530"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>420)
                     /locus_tag="ACIE68_005209"
     CDS             complement(<1..>420)
                     /locus_tag="ACIE68_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFX5911321.1"
                     /translation="LQYNLSVEHFNLNSSLIYYINNWNLFPLFCLFSGYHFYRERFAE
                     RGFFYKVPAVLRDYLSAIPVKINEKARYKPGIASYHNIITCGFSTLSPYIRQQPLAMQ
                     QRFNLLFPDFVDHIQLPLPLASTLLERITFYAKKNRDE"
BASE COUNT          144 a           69 c           75 g          132 t
ORIGIN      
        1 ctcatctcta ttttttttcg cataaaatgt aattctctct aaaagagttg atgcaagtgg
       61 taacggcaat tgtatgtgat caacaaaatc aggaaataat aaattaaatc gttgctgcat
      121 tgccaatggt tgttgcctta tatagggtga caacgttgaa aaaccacatg tgatgatatt
      181 gtgatagcta gcgatgccgg gtttgtatcg tgccttttca tttattttca ccggtatagc
      241 tgataaataa tcccttagca cagcaggaac cttataaaaa aatccgcgtt ctgcaaacct
      301 ttctcgataa aagtgataac cactgaataa acagaacaat ggaaataaat tccaattgtt
      361 tatgtagtaa ataagtgaac tattcagatt aaaatgttca actgaaagat tgtactgcag
//
LOCUS       DBANUU010000522          417 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.531, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000522 DBANUU010000000
VERSION     DBANUU010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.531"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..274)
                     /locus_tag="ACIE68_005210"
     CDS             complement(<1..274)
                     /locus_tag="ACIE68_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraJ"
                     /protein_id="HFX5911322.1"
                     /translation="MCAMDRRERALIRQLHFPNDLRENISLLKYPVCIRFEDGSFIGT
                     NHSFERFIKLSYCSCDEWFEHLTLESKLQLSRSEMDSYFSTYGVSYI"
BASE COUNT          144 a           77 c           66 g          130 t
ORIGIN      
        1 taatataact tacaccataa gttgaaaaat atgaatccat ctcagatctt gatagttgta
       61 acttggattc taaagtcaaa tgctcaaacc attcatcaca tgaacaatat gataatttaa
      121 taaatcgctc gaagctgtga tttgttccta taaaagaacc gtcttcaaaa cgtatacata
      181 cgggatattt caataatgaa atgttctctc ttaagtcgtt agggaaatgt aattgacgaa
      241 tgagtgctct ttctctacga tccattgcac acataggaac ctcctcaaag gattctatgg
      301 acagtcaatg cagggagggg ctactccctg cattgacgat tcaaactaaa tttttaacgt
      361 ggcattaacc acataattaa tataaccttt atgtggttaa ttgtcatcaa attgaac
//
LOCUS       DBANUU010000523          415 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.532, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000523 DBANUU010000000
VERSION     DBANUU010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.532"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>415
                     /locus_tag="ACIE68_005211"
     CDS             <1..>415
                     /locus_tag="ACIE68_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911323.1"
                     /translation="RLALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLR
                     GRISGTQFIRNLAVASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASA
                     VSGKIAGALVEEDRVKILAMIQEQVTWLAGSFLLTG"
BASE COUNT           86 a           93 c          140 g           96 t
ORIGIN      
        1 cgactggctc ttcagaaggg gaccggtgca ggaaatatca gtgccctgaa caaagtgatg
       61 aagggtacgc tggtgacatc tctggcactg gtagctgtca caaccggccc tgacatgatc
      121 aaaatgttgc ggggacggat ctccggtacg cagttcatca ggaatcttgc cgtggcatct
      181 tccggtgtgg caggtggtgc tgtcgggtca gtggcgggcg gaatactgtt cagtccactg
      241 ggaccatttg gtgcactgac agggcgtgtg gttggcggtg ttctgggggg aatgattgcc
      301 tccgctgtat caggaaaaat tgccggagcg ctggttgaag aagatcgcgt caaaattctg
      361 gcaatgattc aggagcaggt gacatggctt gccggcagtt tcctgctgac cggac
//
LOCUS       DBANUU010000524          415 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.533, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000524 DBANUU010000000
VERSION     DBANUU010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.533"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..330
                     /locus_tag="ACIE68_005212"
     CDS             <1..330
                     /locus_tag="ACIE68_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFX5911324.1"
                     /translation="VRVADIVQLNGNIRPRSRQWWQLFRMVSQWHVDVVIVERRSFSI
                     VAAVELDDASHLRPERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWLNTTGADQ
                     QSPEHRS"
BASE COUNT           96 a          103 c          123 g           93 t
ORIGIN      
        1 gtacgggtcg cggatatcgt ccagctgaac gggaatatcc ggccacgatc acgccagtgg
       61 tggcagttat tcaggatggt gtctcagtgg catgttgatg tggttatcgt tgagcggcgt
      121 tcgttcagta ttgttgcagc agtagagctg gatgatgcca gccatttacg accggaacgc
      181 agacgccggg atattcttct ggaagaggtt ctgaggcagg ctggtattcc gttgctcaga
      241 agccacgatg ccagaaaact gctgcagatg accggagaat ggctgaatac aacaggggct
      301 gatcagcagt ccccggaaca tcgtagctga cgccttcgcg ttgctcagtt gtccaacccc
      361 ggaaacggga aaaagcaagt tttccccgct cccggcgttt ccataactga aaacc
//
LOCUS       DBANUU010000525          414 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.534, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000525 DBANUU010000000
VERSION     DBANUU010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.534"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..101)
                     /locus_tag="ACIE68_005213"
     CDS             complement(<1..101)
                     /locus_tag="ACIE68_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911325.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCAL"
     gene            complement(82..>414)
                     /locus_tag="ACIE68_005214"
     CDS             complement(82..>414)
                     /locus_tag="ACIE68_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFX5911326.1"
                     /translation="VVTLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLKEVQEAVML
                     IEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFESGLRQSTEE
                     EKSDSRAA"
BASE COUNT           86 a          130 c          103 g           95 t
ORIGIN      
        1 agcgcacaaa agaaaacccg cgcataggcg ggttcaagca tcagcggctc attaaaagcc
       61 atgctggcaa tatgcgggag attacgcagc tctgctgtca ctcttctcct cctctgttga
      121 ttgtcgcagt ccggattcaa atgccgcagc cgcccaggcg ggcggtttaa gaccggctgc
      181 acggcgctcc atcgtttcac ggacctgctg ggcaaaaatt tcctgatagt cgtcaccgcg
      241 tttcgcgcac tctttctcgt aggtgctcag tccggcttct atcagcatca ccgcttcctg
      301 aacttctttc agaccatcga tggccatacg accggagcct atccagtcgc agttccccca
      361 ggcactgcgg gcttcctgaa agctgaagcg cgcttttgaa ggtaacgtca ccac
//
LOCUS       DBANUU010000526          412 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.535, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000526 DBANUU010000000
VERSION     DBANUU010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.535"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..89
                     /locus_tag="ACIE68_005215"
     rRNA            <1..89
                     /locus_tag="ACIE68_005215"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            158..234
                     /locus_tag="ACIE68_005216"
     tRNA            158..234
                     /locus_tag="ACIE68_005216"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:192..194,aa:Ile,seq:gat)
     gene            277..352
                     /locus_tag="ACIE68_005217"
     tRNA            277..352
                     /locus_tag="ACIE68_005217"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:310..312,aa:Ala,seq:tgc)
BASE COUNT          107 a           91 c          110 g          104 t
ORIGIN      
        1 ggagggcgct taccactttg tgattcatga ctggggtgaa gtcgtaacaa ggtaaccgta
       61 ggggaacctg cggttggatc acctccttac cttaaagaag cgtactttgt agtgctcaca
      121 cagattgtct gatgaaaatg agcagtaaaa cctctacagg cttgtagctc aggtggttag
      181 agcgcacccc tgataagggt gaggtcggtg gttcaagtcc actcaggcct accaaatttg
      241 caccgcaaat ttgaagaggt tttaactaca tgttatgggg ctatagctca gctgggagag
      301 cgcctgcttt gcacgcagga ggtctgcggt tcgatcccgc atagctccac catctctgta
      361 gtgattaaat aaaaaatact tcagagtgta cctgcaaagg ttcactgcga ag
//
LOCUS       DBANUU010000527          409 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.536, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000527 DBANUU010000000
VERSION     DBANUU010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.536"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..205)
                     /locus_tag="ACIE68_005218"
     CDS             complement(<1..205)
                     /locus_tag="ACIE68_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFX5911327.1"
                     /translation="MATTCSVILILESFDVYFGKESVFLERGSSVLVDSSSRDFFLTY
                     PERVIVADFGAEFISRYLKANNLR"
BASE COUNT          168 a           87 c           53 g          101 t
ORIGIN      
        1 cccttaagtt attagctttc aaatagcgac taataaactc agcgccaaaa tccgccacta
       61 tcactctttc aggatatgtc aggaaaaaat ctctagagct agagtcgaca agtacagatg
      121 aacctctctc cagaaacaca ctctcttttc cgaaataaac atcaaaggac tccaaaatca
      181 atataactga acatgtagtt gccatatcat ccacccaatt taactgaaac caggatgaag
      241 tatatgcata taaagttcac tttgccaaac ttcagtataa aaaaccacat aaaaaatagg
      301 gtgtgataaa aaataccgta acaataaagc aaaggattat aaaatccgtt acagttacaa
      361 atgatactca agaaacaatt ccttaggaaa aacttattta cagccaata
//
LOCUS       DBANUU010000528          407 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.537, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000528 DBANUU010000000
VERSION     DBANUU010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.537"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>407)
                     /locus_tag="ACIE68_005219"
     CDS             complement(<1..>407)
                     /locus_tag="ACIE68_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFX5911328.1"
                     /translation="AASGGASASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIVHRG
                     EFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEGINFNQNNNVVIQND
                     GTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGG"
BASE COUNT           82 a          143 c           88 g           94 t
ORIGIN      
        1 ccaccatcac gcatctgtgt ctgaatttca tcacgggccc ccttgcgggc catgtcatac
       61 actgccttca tcatctgtgg acctggcaga ccattcgtac cgtcgttctg aatcaccacg
      121 ttgttgttct gattaaaatt aatgccttca gcccgccgca tctgcgccgg acttccggca
      181 ccgcccacat aaccaccttc cgcatagccc cgcatcaggc ggtacaggtt gccgacacca
      241 atccggctgg ttgcctcctt cgtgaagaca aattcaccac gatgaacaat ccccgctggc
      301 tcatatttgc cgccggttcc cgtaaatccc ccggtcgcaa aatggaattt cgccgcagct
      361 gcctgaatgg ctgtaccgcc tgacgctgat gctccaccac tggcagc
//
LOCUS       DBANUU010000529          406 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.538, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000529 DBANUU010000000
VERSION     DBANUU010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.538"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
BASE COUNT          114 a          100 c           84 g          108 t
ORIGIN      
        1 aataacttct tacaaagtct attattaata ccgtaaatat tcatgcttct taccctgtgc
       61 accaaaagca aaaacttatc gatgcgggtt tgaaactgat ggcaaccgca aaaatgcctg
      121 atgcgctacg cttatcaggc ctacgccatc tctgcaatat attgaatttg cgtgcttttg
      181 taggcaggat aaggcgttca cgccgcatcc ggcatgaata aagcgcactc aacaatctga
      241 aacccgccgg aacggtttat tacgtacatc aggtgaaact gaccgataag ccgctttctt
      301 ttgggtatag cgtcgtggac agtcattcat ctttctgccc ctccaaaagc aaaaacccgc
      361 cggtttacgc tggcgggttt tcgttaaatc aggtgaaact gaccga
//
LOCUS       DBANUU010000530          406 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.539, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000530 DBANUU010000000
VERSION     DBANUU010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.539"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..350
                     /locus_tag="ACIE68_005220"
     CDS             <1..350
                     /locus_tag="ACIE68_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFX5911329.1"
                     /translation="LLEAGAKGCVWKTSHPAKLNRAIDSISNGYTYFDSVHMDCEKIS
                     SRYSSDNQLTNRESEILQLIADGKTNKEIANFLQLSRKTVETHRLNIMKKLDVHSGIE
                     LIKTALRMGVCTI"
BASE COUNT          143 a           65 c           79 g          119 t
ORIGIN      
        1 aattgcttga agcaggtgcg aaaggatgcg tatggaaaac cagtcacccg gcgaaactaa
       61 atcgtgctat tgattcgatt agtaatggtt acacttattt tgatagtgta catatggatt
      121 gtgaaaaaat atcttccagg tattcctctg ataatcaact tacaaatcgt gagtcagaaa
      181 tattacaact catagctgat ggtaaaacaa acaaagaaat cgctaatttc ttgcagttaa
      241 gcagaaaaac agtcgaaact catagactca atatcatgaa gaaattagat gttcacagtg
      301 gtatcgagtt gatcaaaaca gctttacgta tgggtgtttg cactatataa aaaaaagagg
      361 ctttaggctc attttgctga agccttacga ttgtctagtt cattaa
//
LOCUS       DBANUU010000531          401 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.540, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000531 DBANUU010000000
VERSION     DBANUU010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.540"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>401
                     /locus_tag="ACIE68_005221"
     CDS             <1..>401
                     /locus_tag="ACIE68_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFX5911330.1"
                     /translation="DAKKTAAVVEQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDV
                     ATQLAGGQSPWLILLQQGGQVKDSFGGMIPMFRGLAGAITLPMVGATSLAVATGALAY
                     AWYQGNSTLSDFNKTLVLSGNQAGLTADRIL"
BASE COUNT           78 a          112 c          133 g           78 t
ORIGIN      
        1 tgatgcgaaa aaaacagcgg cagtcgttga acagtcgctg agccgacagg cgctggctgc
       61 acagaaagcg gggatttccg tcgggcagta taaagccgcc atgcgtatgc tgcctgcaca
      121 gttcaccgac gtggccacgc agcttgcagg cgggcaaagt ccgtggctga tcctgctgca
      181 acagggtggt caggttaagg actccttcgg cgggatgatc cccatgttca gggggcttgc
      241 cggtgcgatc accctgccga tggtgggggc cacctcgctg gcggtggcga ccggtgcgct
      301 ggcgtatgcc tggtatcagg gcaactcaac cctgtccgat ttcaacaaaa cgctggtcct
      361 ttccggcaat caggcgggac tgacggcaga tcgtattctg g
//
LOCUS       DBANUU010000532          387 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.541, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000532 DBANUU010000000
VERSION     DBANUU010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.541"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..273
                     /locus_tag="ACIE68_005222"
     CDS             <1..273
                     /locus_tag="ACIE68_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFX5911331.1"
                     /translation="HLIRSNAIASTPVYDNYFNNTTAFRPKPEDIFKCGQTLKKTKQK
                     YGHYGLGVDQHQKIDNYTMGLNSWKIAPNERGDKKGVPGLSCFQPH"
BASE COUNT          127 a           73 c           70 g          117 t
ORIGIN      
        1 catctaatta gaagcaatgc tatagcatcc acacctgtat atgacaatta ttttaataac
       61 actactgctt tcagaccaaa acctgaagat atcttcaaat gtggacagac gctcaaaaaa
      121 actaaacaga aatatggcca ttatggatta ggggtagatc agcatcaaaa aattgataat
      181 tataccatgg ggctaaattc ctggaagata gctcctaacg aacgtggtga taaaaaaggt
      241 gtcccagggc ttagttgttt tcaaccacat taaaactcca tgagtgctgt ggtatatctt
      301 tttaatcctg ccagtatttc ataggttttc ttaatcgatt gtagaggcat gttctgatga
      361 cgcctctaca atctttaggg actttct
//
LOCUS       DBANUU010000533          384 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.542, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000533 DBANUU010000000
VERSION     DBANUU010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.542"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>384
                     /locus_tag="ACIE68_005223"
     CDS             <1..>384
                     /locus_tag="ACIE68_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911332.1"
                     /translation="TRKNIPELSELPPSLRKKNFTDAYNAASSHIEYWIGAGVSFISM
                     MILFKVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILERGKETE
                     TISERLIREADTREYELWRPAHRFF"
BASE COUNT          115 a           74 c           74 g          121 t
ORIGIN      
        1 ggacaagaaa aaacattcct gaactttcag aacttccacc ttcattaaga aagaaaaatt
       61 ttactgacgc atataatgct gcatcttccc atatcgaata ctggataggt gctggcgtct
      121 catttatctc gatgatgatt ttattcaaag tatatgactt tcttttaccc gcccaagata
      181 ctttcccggg tgatatcata agaagtttat gtgttgtgtg tccatccata ttaatctggt
      241 ttcagttttc ggtatatgtc atgaggaaat attacagaca tattctggaa cgaggtaagg
      301 aaaccgaaac catttcagaa aggttgatac gtgaggctga tacacgagaa tatgaactat
      361 ggcgtccggc ccatcgcttt ttct
//
LOCUS       DBANUU010000534          383 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.543, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000534 DBANUU010000000
VERSION     DBANUU010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.543"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            74..181
                     /locus_tag="ACIE68_005224"
     CDS             74..181
                     /locus_tag="ACIE68_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFX5911333.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(196..>383)
                     /locus_tag="ACIE68_005225"
     CDS             complement(196..>383)
                     /locus_tag="ACIE68_005225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911334.1"
                     /translation="MFAMKSAFRFFNSCYSRFMSALDDASSSGCQYLLVEVIKTKMAA
                     GCYPCGRFRAHIAITAA"
BASE COUNT          104 a           96 c           99 g           84 t
ORIGIN      
        1 aacgggggct aatcttgaaa ccagacactt caagaatagc cgcaaaatgt tgtcattaca
       61 acaaggcggc tatatgacgc tcgcgcagtt tgccatgact ttctggcacg acctggcggc
      121 accgatcctg gcgggaatta ttaccgcagc gattgtcggc tggtggcgta accggaagta
      181 aggtgaaaac gggcgtcagg ctgccgtgat ggcaatatgt gcccgaaacc ggccgcaggg
      241 gtaacagcct gcggccattt ttgtctttat cacttccaca agtaaatatt ggcaacccga
      301 gctggaagca tcatccagag cactcatgaa acgactgtaa cagctgttga aaaaccggaa
      361 tgcactcttc attgcaaaca ttt
//
LOCUS       DBANUU010000535          376 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.544, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000535 DBANUU010000000
VERSION     DBANUU010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.544"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..101
                     /locus_tag="ACIE68_005226"
     rRNA            <1..101
                     /locus_tag="ACIE68_005226"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            273..348
                     /locus_tag="ACIE68_005227"
     tRNA            273..348
                     /locus_tag="ACIE68_005227"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:307..309,aa:Glu,seq:ttc)
BASE COUNT           92 a           89 c          103 g           92 t
ORIGIN      
        1 gcttaacctt cgggagggcg cttaccactt tgtgattcat gactggggtg aagtcgtaac
       61 aaggtaaccg taggggaacc tgcggttgga tcacctcctt accttaaaga agcgttcttt
      121 gcagtgctca cacagattgt ctgatgaaaa gtgaatagca aggcgtttac gcgttgggag
      181 tgaggctgaa gagaataagg ccgttcgctt tctattaatg aaagctcacc ctacacgaaa
      241 atatcacgca acgcgtgata agcaattttc gtgtcccctt cgtctagagg cccaggacac
      301 cgccctttca cggcggtaac aggggttcga atcccctagg ggacgccact tgctggtttg
      361 tgagtgaaag tcgccg
//
LOCUS       DBANUU010000536          370 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.545, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000536 DBANUU010000000
VERSION     DBANUU010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.545"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..285
                     /locus_tag="ACIE68_005228"
     CDS             <1..285
                     /locus_tag="ACIE68_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HFX5911335.1"
                     /translation="SDEDYKKTITDDTKLIHYVGVTKPWNDWTNYPCQKYFNEAYQAS
                     CWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNKYKRKLFG"
BASE COUNT          139 a           52 c           70 g          109 t
ORIGIN      
        1 agcgatgaag attataaaaa gaccattacc gatgatacta agctgattca ttatgttggc
       61 gtaacgaagc cctggaacga ctggacgaat tatccctgcc agaagtattt taatgaggct
      121 tatcaggctt cttgctggaa tgatgtggcg tttattccag ccacgaatga aaagcagtat
      181 caagtgaaat atcaacatgc aaagaaaaat ggtgatacgt ttaacgcttt tatttacttc
      241 attaaattta aattaaataa gtataaaaga aaactatttg gataataata aaaatcatgg
      301 atgtctgagt tacaataata attaaggatg agtactatca tattttatta gttcaggcaa
      361 gagaacacga
//
LOCUS       DBANUU010000537          365 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.546, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000537 DBANUU010000000
VERSION     DBANUU010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.546"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>365
                     /locus_tag="ACIE68_005229"
     CDS             <1..>365
                     /locus_tag="ACIE68_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFX5911336.1"
                     /translation="NQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYA
                     MGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRK
                     GNCYDNAPMESFWGTLKNE"
BASE COUNT           98 a           90 c          102 g           75 t
ORIGIN      
        1 aaccagacgt tcgctcctac agcaccaaat caggtctggg tggcggacct gacgtatgtt
       61 gccacacagg agggatggtt gtacctcgct ggcatcaaag atgtttatac gtgcgaaatt
      121 gtcggctacg ccatgggaga gcgcatgaca aaagagctga caggtaaagc cctgtttatg
      181 gcgctcagga gccagcgccc acctgccggg ctaatccacc actctgatcg aggttcacag
      241 tactgcgcat acgattaccg ggtcatacag gagcagtttg gtctgaaaac atcaatgtcg
      301 cgtaaaggta actgttacga caacgctccg atggaaagct tctggggaac gctgaaaaat
      361 gagag
//
LOCUS       DBANUU010000538          365 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.547, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000538 DBANUU010000000
VERSION     DBANUU010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.547"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>365
                     /locus_tag="ACIE68_005230"
     CDS             <1..>365
                     /locus_tag="ACIE68_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HFX5911337.1"
                     /translation="SARTTDRSIIRAMMEGGTAKIYHCNDSDKCLKVVADTPVTISRD
                     NALKSQITKLLASIQNKAVSDTPLDDKEKGFISSTTIPVFKYLVDPQMLGVSNSMIYQ
                     LTDYIGYDILLQYIQELIQ"
BASE COUNT          105 a           96 c           83 g           81 t
ORIGIN      
        1 tcagccagaa ccactgaccg ctcaattatc cgggccatga tggaaggcgg cacagcaaaa
       61 atttatcact gcaacgattc tgataaatgc ctgaaagtgg tggcagacac accggtgacc
      121 atcagccggg ataatgcact gaagtctcag attactaaac ttctggccag cattcagaac
      181 aaggcggtca gtgacacgcc tctggatgac aaggaaaaag gctttatttc cagtaccacc
      241 atccccgtct tcaaatacct ggttgacccg cagatgctcg gtgtttccaa cagtatgatt
      301 taccagctga cggactatat cggttacgac atcctgctgc agtacattca ggagctgata
      361 cagca
//
LOCUS       DBANUU010000539          362 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.548, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000539 DBANUU010000000
VERSION     DBANUU010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.548"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>362
                     /locus_tag="ACIE68_005231"
     CDS             <1..>362
                     /locus_tag="ACIE68_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911338.1"
                     /translation="QPVLNSKSWLTSLQNDYSLPDSLTERIWLTLISQRISRGELREF
                     ELADGNWLLNNAWYERNMAGFNEQLKENLSFTPDELKTLFRNRLNLSPEANDDFLDRC
                     LDGGDWYPFSEGRRFVSF"
BASE COUNT           96 a           87 c           91 g           88 t
ORIGIN      
        1 caaccggtgc tcaacagtaa atcgtggctg accagtttgc aaaacgatta cagccttcct
       61 gactcactga cggagcgcat ctggctgacg ctcatttctc aacggatttc ccggggagag
      121 ctgagggaat ttgaactggc agacggaaac tggttactga acaatgcctg gtatgaaaga
      181 aacatggcag ggtttaacga acagttgaaa gagaacctgt cattcacacc tgatgaactg
      241 aaaacgctct tccggaaccg cctgaattta tcaccggaag cgaatgacga ttttctcgat
      301 cgttgcctgg acggcggtga ctggtatccc ttttcagaag gtcgccgttt tgtatcattc
      361 ca
//
LOCUS       DBANUU010000540          359 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.549, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000540 DBANUU010000000
VERSION     DBANUU010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.549"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..351
                     /locus_tag="ACIE68_005232"
     CDS             <1..351
                     /locus_tag="ACIE68_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFX5911339.1"
                     /translation="DSRKAVRIRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYF
                     QRDRYGRILGQVYAPDGMNVNHFMVRAGAAWVYEQYNTDPVLPVFQNEARQQKRGLWS
                     DADPVPPWIWRHRK"
BASE COUNT           98 a           78 c          102 g           81 t
ORIGIN      
        1 gactcacgga aggcggtgcg gatcaggctg gtaaatattg atgcaccgga aaagaaacag
       61 gattatggcc ggtggtcaac ggacatgatg aaatcactgg ttgcaggaaa aacggttacc
      121 gtcacatatt ttcagcgaga tcgttatggt cgtattctcg ggcaggttta tgcaccagat
      181 ggaatgaatg ttaatcactt tatggtccgg gccggggctg cctgggtata tgagcagtac
      241 aacacagatc ccgtgctacc tgtctttcag aacgaagcca gacaacaaaa acgtgggctc
      301 tggtcagatg ctgatccggt tcccccctgg atatggagac accgtaaata aagcgctgg
//
LOCUS       DBANUU010000541          358 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.550, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000541 DBANUU010000000
VERSION     DBANUU010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.550"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..269
                     /locus_tag="ACIE68_005233"
     CDS             <1..269
                     /locus_tag="ACIE68_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFX5911340.1"
                     /translation="LLSRFQPDRIKISQELITNVHKSGPRQAIIQAIIKCCTSLEIQV
                     SAMGVATPEEWMWLESAGIEMFQGDLFAKAKLNGIPSIAWPEKK"
BASE COUNT          117 a           67 c           73 g          101 t
ORIGIN      
        1 tactcctgtc acgcttccag cctgacagaa ttaaaatcag tcaggaattg attaccaatg
       61 ttcataaaag tgggccacgg caggcaatta ttcaggcgat cataaaatgc tgtacatccc
      121 ttgaaattca agtcagtgct atgggcgtgg caacaccaga agagtggatg tggcttgaat
      181 ccgcaggaat tgagatgttt cagggagatc tgtttgctaa agctaaattg aatggtatcc
      241 cttccatagc gtggccagag aaaaaataat tttcagcaca ttctttcaca tgatttcagt
      301 aaatcatcgt gaaaatgaat acgttacgtt taaatttaat ccacattaaa aataatgt
//
LOCUS       DBANUU010000542          356 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.551, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000542 DBANUU010000000
VERSION     DBANUU010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.551"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>356
                     /locus_tag="ACIE68_005234"
     CDS             <1..>356
                     /locus_tag="ACIE68_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFX5911341.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEACDENTIGVVP"
BASE COUNT           81 a           94 c          106 g           75 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgcc
//
LOCUS       DBANUU010000543          353 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.552, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000543 DBANUU010000000
VERSION     DBANUU010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.552"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>353
                     /locus_tag="ACIE68_005235"
     CDS             <1..>353
                     /locus_tag="ACIE68_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911342.1"
                     /translation="LLFRALISTASALAGLILLCLFGPALLAGYITERITRLFHIRWL
                     AGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTTALAGGLLAVPLQIKNIQQN
                     GITPEDISKEINGYY"
BASE COUNT           78 a           91 c           89 g           95 t
ORIGIN      
        1 gccttttgtt cagagcatta atcagcacgg cttctgcgct ggcagggctc atattgctgt
       61 gtctgttcgg cccggcctta ctggctggct atatcaccga acgcataacc cggttgttcc
      121 atattcgctg gctggcaggc gtatttctga cgattgccgg aatgatcatc agcttcatgt
      181 ggggacttga tggtaaacat atcgcgctgg aggctcacac ctttgactct gtgaaattta
      241 ttctgaccac cgctctcgcc ggtggtctgc tggctgttcc cctgcagatc aaaaacattc
      301 agcagaacgg gatcacacca gaagatatca gcaaggaaat taacgggtat tac
//
LOCUS       DBANUU010000544          353 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.553, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000544 DBANUU010000000
VERSION     DBANUU010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.553"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            39..290
                     /gene="trbG"
                     /locus_tag="ACIE68_005236"
     CDS             39..290
                     /gene="trbG"
                     /locus_tag="ACIE68_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HFX5911343.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRSTVFENPDIVKTCTVRDLKKDFVNCDEKGEGQ"
     gene            287..>353
                     /locus_tag="ACIE68_005237"
     CDS             287..>353
                     /locus_tag="ACIE68_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraV"
                     /protein_id="HFX5911344.1"
                     /translation="MKQISFFIPLLGTLLLSGCAGT"
BASE COUNT          104 a           61 c           90 g           98 t
ORIGIN      
        1 ggtgtgatga agaattcaga aatataagga acccttcgat gaataagctg gtttctgatg
       61 gcagcgtcaa aaaaataaat tatccggtgc tgtatgagtc cggaataaca cctccgttat
      121 gtgaagtcag tgcaccagaa cctgatgcgg gtgggaaacg cattgtggca tatgtttata
      181 aatcatcacg gagtacggtt tttgaaaatc ctgacattgt aaaaacctgt acggtcaggg
      241 atttgaagaa ggattttgtg aactgcgatg agaaaggtga gggacaatga aacagatttc
      301 tttctttatt cctctgctgg ggaccttatt actttccggc tgtgccggaa cca
//
LOCUS       DBANUU010000545          351 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.554, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000545 DBANUU010000000
VERSION     DBANUU010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.554"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            103..>351
                     /locus_tag="ACIE68_005238"
     CDS             103..>351
                     /locus_tag="ACIE68_005238"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911345.1"
                     /translation="MEIVIYITISILILDIALIKQRMLANIETSNPYHAQYILRRRDD
                     YLVLFDRDALSCRFFYDAFPGMRLRHQVTGDTDDDRLAH"
BASE COUNT           86 a           94 c           83 g           88 t
ORIGIN      
        1 gttttgttat aaggtaatcg gtttctttac agaccgctgg cgtacatcgc acagaggctg
       61 ctaacaggga tattcctgcc tggatgaacg caaggcaaga tcatggaaat agtgatatac
      121 atcactattt ccatcctgat tttagatatc gcattaataa aacagcggat gttagccaat
      181 atcgaaacca gtaacccgta tcacgcccag tacatcctgc gccgccgcga tgattacctg
      241 gtgctgttcg accgtgatgc cctgagctgc cgcttctttt atgacgcctt tccgggaatg
      301 cgtctgcgcc accaggtgac cggcgatacc gacgatgacc gcctggcaca c
//
LOCUS       DBANUU010000546          350 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.555, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000546 DBANUU010000000
VERSION     DBANUU010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.555"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..182
                     /locus_tag="ACIE68_005239"
     CDS             <1..182
                     /locus_tag="ACIE68_005239"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911346.1"
                     /translation="TPDDLSLLPWLFTTAAGVVWDRLPTADPGGPATISSQAYAYYML
                     HAKRPVLHLCAYGTP"
BASE COUNT           85 a           88 c           63 g          114 t
ORIGIN      
        1 gaactcctga tgatctttcc ctgctaccat ggctctttac cacagcagcc ggggttgttt
       61 gggaccgact tccgacagca gatcccggtg ggcctgccac catctcttca caagcttatg
      121 cttattacat gttacatgca aaacgtcccg ttttgcatct ttgtgcctac ggcacaccgt
      181 gatctttccc tgatgaggga tatattaact attgaacatt agcgcgaatt atttagtgca
      241 aaaaatcagc atcacgtgat gctttccctg ctattaataa cctcctctat ttaactatat
      301 ttttcattga tgcaattcgt agatttgaag aagttcaact gccacttttt
//
LOCUS       DBANUU010000547          347 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.556, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000547 DBANUU010000000
VERSION     DBANUU010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.556"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>347
                     /locus_tag="ACIE68_005240"
     CDS             <1..>347
                     /locus_tag="ACIE68_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HFX5911347.1"
                     /translation="TKQTGTGNRFVLQNLGNTELSFAAKVWGSSDRQNVFEVGTSAAY
                     LFYAQKTSAGQLFDVNGAINCTTLNQSSDRDLKDDILVISDATKAIRKMNGYTYTLRE
                     NGMPYAGVIAQEV"
BASE COUNT          110 a           70 c           81 g           86 t
ORIGIN      
        1 acaaaacaaa cagggactgg caatcgtttt gttttacaga acttaggtaa tacagaacta
       61 tcatttgccg caaaagtatg gggatcaagt gatcgacaaa acgtttttga ggttggaacg
      121 tctgctgcgt atctgtttta tgcgcaaaaa acgtcagcag gccagttgtt tgatgtaaat
      181 ggcgctatta attgcacaac gctgaatcag tcatcagacc gcgaccttaa agacgatatt
      241 ctcgttatca gcgacgcgac gaaagcaatc cgtaaaatga acggatacac ctacacgctc
      301 agggaaaacg ggatgcctta tgctggcgtt attgcacagg aagtaat
//
LOCUS       DBANUU010000548          341 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.557, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000548 DBANUU010000000
VERSION     DBANUU010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.557"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>341
                     /locus_tag="ACIE68_005241"
     rRNA            <1..>341
                     /locus_tag="ACIE68_005241"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           92 a           83 c           94 g           72 t
ORIGIN      
        1 gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta tacggtgtga
       61 cgcctgcccg gtgccggaag gttaattgat ggggtcagcg caagcgaagc tcttgatcga
      121 agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat tccttgtcgg
      181 gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac ccgagactca
      241 gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa agaccccgtg
      301 aacctttact atagcttgac actgaacatt gagccttgat g
//
LOCUS       DBANUU010000549          337 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.558, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000549 DBANUU010000000
VERSION     DBANUU010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.558"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
BASE COUNT          121 a           75 c           60 g           81 t
ORIGIN      
        1 actcagggga aactcatcac aaggctgacc gaatgacata gtaacctcac tacacacaga
       61 tataaaagca ctgcaattct ggctattaat agcaaaaata gcagtcaaat caatatgagg
      121 taaaaatttt tgttgattta atggttaaat aatagccaca gtgtgttttg ctgctgaaga
      181 aagacaaaat gaatctgtgt gtcccctcct tcccttcaga gcacatccac tattcaaatt
      241 ttacacaacc acgtacgaac aaaaagcatc atcccgggta ttgcagatac cggtttcctg
      301 ccgggaagaa tcataaacag aacaccggga ataacgc
//
LOCUS       DBANUU010000550          333 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.560, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000550 DBANUU010000000
VERSION     DBANUU010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.560"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..151)
                     /locus_tag="ACIE68_005242"
     CDS             complement(<1..151)
                     /locus_tag="ACIE68_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HFX5911348.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDH"
BASE COUNT          122 a           76 c           50 g           85 t
ORIGIN      
        1 gatgatcttt cagataagaa gcgaccgaca attcgcgacc atttaacgtg gcatctccta
       61 atccacaaag tccgctttca gtggttattt taagcgtaac gaaattacgc cctgggcaag
      121 taacaaaaac atctgcagca ataattttca ttattgcaat ctcctgtgtt gtaaatagac
      181 gcttcaaact taattttacg cctactacca tacaagagag aaagagcgat tattcaaccg
      241 tgatcacatt aaaaataaaa atttccgccg aggagaaaaa ataccctcaa aaaccacctg
      301 aataattaac caccatcgtt aaaacaacac gtt
//
LOCUS       DBANUU010000551          330 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.561, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000551 DBANUU010000000
VERSION     DBANUU010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.561"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(103..294)
                     /locus_tag="ACIE68_005243"
     CDS             complement(103..294)
                     /locus_tag="ACIE68_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911349.1"
                     /translation="MNGFRNSSRNGQVWRYQSAGSRAVILEAAEAWLRAAGVTPRTDW
                     QQFARKRAEQCHRRCRGRV"
BASE COUNT           66 a          100 c           73 g           91 t
ORIGIN      
        1 cagaactgtc cggtcatact gcccgaccgg atattaaaaa aagcaaatct catgcattat
       61 tcagccttct gaatccctgg agtaatgaag gctgcttttt cgttacaccc ttccccggca
      121 gcgccggtgg cattgttcag ccctttttcg ggcaaactgc tgccagtctg tccggggtgt
      181 aacaccggca gcccgtagcc atgcttctgc cgcttccaga ataacggccc ggcttccggc
      241 actctggtaa cgccagacct gaccgttccg agaactgttt ctgaatccat tcattttcct
      301 gtcccccctt tagttgtcag tattattgtg
//
LOCUS       DBANUU010000552          327 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.562, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000552 DBANUU010000000
VERSION     DBANUU010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.562"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>327
                     /locus_tag="ACIE68_005244"
     CDS             <1..>327
                     /locus_tag="ACIE68_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911350.1"
                     /translation="NHQDKHSDKVNSHQHHGKVDKTHRAKIVEFDKLDNDSQIDNDFG
                     LHIIYFLQHGHWKVNDRSHQMEKVWFYNSEPSIDIQEYNRFADNTTDTFIFTIIPDNN
                     HVLKLS"
BASE COUNT          122 a           55 c           55 g           95 t
ORIGIN      
        1 caaaccatca agataagcat tcagataaag ttaacagtca tcaacaccat ggcaaggttg
       61 ataaaacaca tcgtgcaaag atagttgaat ttgataaact ggataatgat tcgcaaatag
      121 ataacgattt tggcctgcat atcatctatt tcctgcaaca tggtcactgg aaggtaaatg
      181 atcgtagtca ccagatggag aaagtatggt tttataatag tgaaccttct atagatattc
      241 aagaatataa tagattcgcc gacaatacta ctgatacgtt tatctttaca attataccag
      301 acaataacca tgtgttaaaa ttatcgt
//
LOCUS       DBANUU010000553          327 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.563, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000553 DBANUU010000000
VERSION     DBANUU010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.563"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>327
                     /gene="traN"
                     /locus_tag="ACIE68_005245"
     CDS             <1..>327
                     /gene="traN"
                     /locus_tag="ACIE68_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFX5911351.1"
                     /translation="SHPVRVGNTKFKRHTAMKLRLVVRVKKASYTPYVVWSESCPFSK
                     ELGKLTKTECTEAGGNRTLVKDGQSYSMYQSCWAYRDTYVTQSADKGTCQTYADNPAC
                     TLVSHQC"
BASE COUNT           90 a           86 c           91 g           60 t
ORIGIN      
        1 tctcaccccg tccgggtcgg gaatacaaaa ttcaaacgtc atacggcaat gaagctgcgt
       61 ctggttgtca gggtaaaaaa agcctcgtac accccgtatg ttgtctggtc tgaaagctgc
      121 ccgttcagca aggagctggg gaaactgaca aaaacagaat gtacggaggc tggcgggaac
      181 aggacgctgg tgaaagacgg tcagtcatac agcatgtacc agagctgctg ggcataccgg
      241 gacacgtatg tcacacagtc agcagacaag ggaacctgtc agacctacgc cgacaatcct
      301 gcctgtaccc tggtgtcaca ccagtgc
//
LOCUS       DBANUU010000554          319 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.564, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000554 DBANUU010000000
VERSION     DBANUU010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.564"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            17..>319
                     /gene="traM"
                     /locus_tag="ACIE68_005246"
     CDS             17..>319
                     /gene="traM"
                     /locus_tag="ACIE68_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFX5911352.1"
                     /translation="MARVILYISNDVYDKVNAIVEQRRQEGARDKDISLSGTASMLLE
                     LGLRVYEAQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFE"
BASE COUNT           98 a           46 c           76 g           99 t
ORIGIN      
        1 tgaaaggttt tattttatgg ccagagtaat tttgtatatc agtaatgatg tctatgataa
       61 agtaaatgcg atagttgaac agcgtcgtca ggagggggca agagataaag atataagcct
      121 ttcaggaact gcttcaatgc ttcttgaact ggggcttcgc gtatatgagg ctcagatgga
      181 gcgtaaagag tctgcattta atcagactga gtttaataaa ttacttcttg aatgtgttgt
      241 aaaaacacag tcatcagtag cgaaaatttt gggtattgag tctctaagtc ctcatgtctc
      301 cgggaatcca aagtttgaa
//
LOCUS       DBANUU010000555          317 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.565, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000555 DBANUU010000000
VERSION     DBANUU010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.565"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..297
                     /locus_tag="ACIE68_005247"
     CDS             <1..297
                     /locus_tag="ACIE68_005247"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911353.1"
                     /translation="DSVSVQELKASFCSVKTHSTNNISADIILTRTPHITEFSSDSIT
                     AQIKKNGINIQTGRMVSQKHLIITHDQEGKHLTTWYQQISLRVHSYKKHTEEVR"
BASE COUNT          129 a           63 c           49 g           76 t
ORIGIN      
        1 gacagtgttt cagtacagga attaaaagcc agcttttgct cagtaaaaac acactcaact
       61 aataatatct cagcagatat tatcctgacc aggacacctc acattactga attctcttcc
      121 gacagtatca ctgcacaaat aaaaaagaac gggataaata ttcagaccgg aagaatggtg
      181 tcacaaaagc atttaatcat tacgcatgac caggaaggga aacacttaac cacatggtat
      241 caacagatat cattacgtgt gcactcttat aaaaaacaca cagaagaagt aagataatta
      301 taaacgtaaa tttaata
//
LOCUS       DBANUU010000556          317 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.566, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000556 DBANUU010000000
VERSION     DBANUU010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.566"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..253)
                     /locus_tag="ACIE68_005248"
     CDS             complement(<1..253)
                     /locus_tag="ACIE68_005248"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFX5911354.1"
                     /translation="MSDGKENRYQKFSVLMSVYYKEDPVFFYDAVNSVFENTIPPDDV
                     VIVVDGPIPEGITDVILALREKYELNIVYLSENVGLGRAL"
BASE COUNT          114 a           73 c           34 g           96 t
ORIGIN      
        1 ttagagctct ccctaaacca acgttttcac ttaaataaac aatgttaagt tcatattttt
       61 ctcttagcgc caatattaca tccgtaatac cttccgggat aggtccatca acgacaatga
      121 ccacatcatc tggagggatc gtattctcaa acacactatt aacagcatca taaaaaaaaa
      181 caggatcttc tttatagtac acggacatca aaacactaaa cttttgatat ctattttctt
      241 tcccatcact cataattatt attaaacctt gcccctaagc ttaatcatca aaactggaga
      301 tataaaagaa ataatcc
//
LOCUS       DBANUU010000557          310 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.567, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000557 DBANUU010000000
VERSION     DBANUU010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.567"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..242)
                     /locus_tag="ACIE68_005249"
     CDS             complement(<1..242)
                     /locus_tag="ACIE68_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFX5911355.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDDNVHKLMDLSI"
BASE COUNT           93 a           61 c           66 g           90 t
ORIGIN      
        1 ttaatggata aatccatcaa cttgtggaca ttgtcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcatt gccatcaaga tataattcgt cattgataat ctggaatgcg
      121 acatcgtcgc gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      181 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      241 attttaaact ccttaaaatg attggatcgc attaaaaaag taggatttat cgataaagta
      301 agcaagtgca
//
LOCUS       DBANUU010000558          308 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.568, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000558 DBANUU010000000
VERSION     DBANUU010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.568"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..160
                     /locus_tag="ACIE68_005250"
     CDS             <1..160
                     /locus_tag="ACIE68_005250"
                     /inference="COORDINATES: protein motif:HMM:NF015584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PapG chaperone-binding domain-containing
                     protein"
                     /protein_id="HFX5911356.1"
                     /translation="LDGYSYDEANLQNKYVTQSGRTIKIGSKLYGDVSKVKPGGINGS
                     LVMTFDFQ"
BASE COUNT           98 a           51 c           72 g           87 t
ORIGIN      
        1 tcttgatggt tattcttatg atgaagcgaa tctccaaaat aagtatgtta cccagtcagg
       61 aagaacaatc aaaataggaa gcaagcttta cggagatgtt tcaaaagtga agcccggggg
      121 tattaatggt agtctggtta tgacttttga ctttcagtaa tctgaatttg ttggggactc
      181 ctgagctggt aagtatccgg atgcgtattc atattgaggt agcctgagtt taacggacac
      241 tccttcctga aatagaatgg catcagaagg agctaataat gagcagaaaa acccaacgtt
      301 actctaaa
//
LOCUS       DBANUU010000559          306 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.569, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000559 DBANUU010000000
VERSION     DBANUU010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.569"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>306
                     /locus_tag="ACIE68_005251"
     CDS             <1..>306
                     /locus_tag="ACIE68_005251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911357.1"
                     /translation="IMNIKTINSTLVGIVAVLLFLAVLVLVKVLFAGSRGFEWGNAAD
                     LTSALCNIVIASTALCAAFVANNWFVQNKKLKSLSTSHQLAMKFEMQLWEINSRLYN"
BASE COUNT           88 a           48 c           63 g          107 t
ORIGIN      
        1 aaattatgaa tataaaaact ataaactcta ctcttgtcgg cattgttgca gtacttctgt
       61 ttttagcagt cttagtactc gttaaggttc tattcgctgg tagcaggggg tttgaatggg
      121 ggaacgcagc tgatttaaca agtgcgttat gcaatatcgt gattgcgtct acagctcttt
      181 gtgctgcttt tgtagcaaat aattggttcg ttcagaataa aaaattaaaa tccctttcta
      241 cctcacatca gttggctatg aaatttgaga tgcagttatg ggaaataaat tcacgtttat
      301 ataatg
//
LOCUS       DBANUU010000560          305 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.570, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000560 DBANUU010000000
VERSION     DBANUU010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.570"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            19..>305
                     /locus_tag="ACIE68_005252"
     CDS             19..>305
                     /locus_tag="ACIE68_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911358.1"
                     /translation="MQDEPDGYGFDCLTFQIADRMTGNMRQEKTTESIKFNHHWQKGA
                     ALSITYSATGAVHIILFPSTSDDSLATHDSLIVHHSYNVKHITPKKIEKAV"
BASE COUNT          109 a           53 c           56 g           87 t
ORIGIN      
        1 agatttaata taattgatat gcaagatgaa ccagatggtt atggttttga ttgcctgaca
       61 tttcagatcg ctgatcgtat gactggaaat atgcggcagg aaaaaactac cgaaagcata
      121 aagtttaatc atcattggca aaaaggagcc gcactttcaa ttacatatag tgcgacgggg
      181 gctgtgcata taattctttt tccgtctact tcagatgatt cattagcaac acatgattcc
      241 ttgattgtcc atcattcata taacgttaaa cacataacac caaaaaaaat agagaaagca
      301 gtaaa
//
LOCUS       DBANUU010000561          302 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.571, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000561 DBANUU010000000
VERSION     DBANUU010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.571"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..237
                     /locus_tag="ACIE68_005253"
     CDS             <1..237
                     /locus_tag="ACIE68_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PapB/FocB family fimbrial expression
                     transcriptional regulator"
                     /protein_id="HFX5911359.1"
                     /translation="TELIQSCMNGEHFSLLIEISPIRSKKNIMALKEYLVDGYSKQES
                     CERNNVSISYFCLCLKKLNYAHSIVSALKYFYRE"
BASE COUNT          105 a           48 c           52 g           97 t
ORIGIN      
        1 acagagctaa ttcaatcatg tatgaatgga gaacactttt cacttttaat tgaaatatca
       61 ccaatcagaa gtaaaaaaaa catcatggct cttaaggagt atttggttga tggttattca
      121 aagcaagaaa gttgtgaaag gaacaatgtt tccattagtt atttttgcct atgcctaaaa
      181 aaactaaact atgcacacag tattgttagt gctcttaaat atttctaccg tgagtaaact
      241 gtaactgaag tcttgattct tacgcttaga cactgctaag ccgttatggc atagctgata
      301 at
//
LOCUS       DBANUU010000562          295 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.573, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000562 DBANUU010000000
VERSION     DBANUU010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.573"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
BASE COUNT           86 a           68 c           48 g           93 t
ORIGIN      
        1 cggatatcta atgtcataag catcccttta attatcccct caacctcttg taactttccc
       61 actcttgaac tccacatagt ccaaacgccg gatgcaaaaa aatcacacgt gcatttctga
      121 aattgtgatg gaggaagata gtatcaatta tatgtgctta taaacgtccc cgattagcgc
      181 agaagtcacc ggattgttca ggctccaata ggttgagtat gccgtattta ttttccaaaa
      241 taaaaatcga tgctgttgct ccttcataaa atacttttac tctctggacg ctttt
//
LOCUS       DBANUU010000563          290 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.574, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000563 DBANUU010000000
VERSION     DBANUU010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.574"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>290
                     /locus_tag="ACIE68_005254"
     CDS             <1..>290
                     /locus_tag="ACIE68_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFX5911360.1"
                     /translation="DQSLLLDDVANKAGYTKWYFQRLFKKVTGVTLASYIRARRLTKA
                     AVELRLTKKTILEIALKYQFDSQQSFTRRFKYIFKVTPSYYRRNKLWELEAM"
BASE COUNT           87 a           48 c           66 g           89 t
ORIGIN      
        1 agatcagtct ttactgcttg atgatgtggc gaataaagcg ggttatacca agtggtattt
       61 tcagcggctg ttcaaaaaag taacaggggt cacactggct agctatattc gtgctcgtcg
      121 tttgacgaaa gcggctgttg agttgaggtt gacgaaaaaa actatccttg agatcgcatt
      181 aaaatatcaa tttgattccc aacaatcttt tacacgtcga tttaagtaca tttttaaggt
      241 tacaccaagt tattatcggc gtaataaatt atgggaattg gaggcaatgc
//
LOCUS       DBANUU010000564          287 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.575, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000564 DBANUU010000000
VERSION     DBANUU010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.575"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>287
                     /locus_tag="ACIE68_005255"
     CDS             <1..>287
                     /locus_tag="ACIE68_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFX5911361.1"
                     /translation="EVFANVEKQLRMIQCLRAVALELITFLAQIENFQKKLWTKKKFV
                     VGHEYLVSLSNLSDSLYDKIRTNKGQHDEWVELYAVNKIPGYSFPFTKDFL"
BASE COUNT           89 a           43 c           58 g           97 t
ORIGIN      
        1 agaagtgttt gccaatgttg agaaacaatt gcgcatgatt caatgtttgc gtgctgttgc
       61 gctggaactc attacatttt tggcacaaat tgagaatttc cagaaaaaac tttggactaa
      121 gaagaagttt gttgttgggc atgagtatct tgtgtcactt tcaaatctat cagattcgct
      181 ttacgataaa attcgaacta ataaagggca acatgatgaa tgggttgagc tttatgctgt
      241 aaataaaatt cctggatatt catttccatt cacaaaagat ttcttgt
//
LOCUS       DBANUU010000565          287 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.576, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000565 DBANUU010000000
VERSION     DBANUU010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.576"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..204)
                     /locus_tag="ACIE68_005256"
     CDS             complement(<1..204)
                     /locus_tag="ACIE68_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HFX5911362.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQ"
BASE COUNT           64 a           67 c           82 g           74 t
ORIGIN      
        1 ctggcaggcg aagaatggct gaaagccttc ccgctgcagg ctttccagta cggtgatggt
       61 ggggatgtac gtatagttct ggcttcggga cgtgtgttta tcttccccga aaatccccgg
      121 cacatagtgc atcagttctt cgtgtgtcag cggacggtca cggcgtatct ggttcgcata
      181 accaaaacga ctggctagtc gcataatttg ctccttatcg gtggttaaga tttactggtg
      241 taataaatga aaaagccacg tctcccggag aagacgcggc ctgatgg
//
LOCUS       DBANUU010000566          285 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.577, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000566 DBANUU010000000
VERSION     DBANUU010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.577"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..260)
                     /locus_tag="ACIE68_005257"
     CDS             complement(<1..260)
                     /locus_tag="ACIE68_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFX5911363.1"
                     /translation="MNQKLIQTVISECHRIEEDALYSSKSHYNVSDRWDKLNLWFGVP
                     LAILTALSGIAAIKSTANAVVAISITATILTALNTSLNPSKRA"
BASE COUNT           91 a           60 c           55 g           79 t
ORIGIN      
        1 gcacgcttac ttgggttaag agacgtattt aatgcagtta gaattgtagc agttatggaa
       61 atagctacaa cagcattggc tgtactcttg attgcggcaa taccactcaa agcggttaag
      121 attgctaatg gtacaccgaa ccacagattc agcttatccc atctatcaga aacattataa
      181 tgagatttag aagagtacag agcgtcctct tctatacgat gacactcaga aatgactgtt
      241 tggatgagct tctgattcat aaacactcca acaaatccga ttccc
//
LOCUS       DBANUU010000567          284 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.578, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000567 DBANUU010000000
VERSION     DBANUU010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.578"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>284
                     /locus_tag="ACIE68_005258"
     CDS             <1..>284
                     /locus_tag="ACIE68_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFX5911364.1"
                     /translation="EEPQKIREIIRDKYLRDTSVLILLVGTETKNRKHVDWELYSSMR
                     DSPRNGKSGVFVINLPSTGTNYVRASHGENEKNELHPNITEWVTINDRAT"
BASE COUNT          110 a           42 c           57 g           75 t
ORIGIN      
        1 ggaagaacct caaaagataa gagaaataat aagggataaa tatttacgtg atacatccgt
       61 tcttatttta cttgttggaa ctgagactaa aaacagaaag catgttgatt gggagttata
      121 ttcatccatg agagatagtc ccagaaacgg taaatcaggt gtttttgtta taaaccttcc
      181 ttcaacagga acaaactacg ttagggccag tcatggtgaa aatgaaaaaa atgaacttca
      241 tcctaatata acggagtggg taacaatcaa tgatagagca acat
//
LOCUS       DBANUU010000568          281 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.579, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000568 DBANUU010000000
VERSION     DBANUU010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.579"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>281)
                     /locus_tag="ACIE68_005259"
     CDS             complement(<1..>281)
                     /locus_tag="ACIE68_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFX5911365.1"
                     /translation="KLSDGWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHIVPRVAGA
                     KSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGMTGILFSLGASM"
BASE COUNT           65 a           94 c           75 g           47 t
ORIGIN      
        1 catactggca ccgagagaaa acaggatgcc ggtcatacca ccggccccaa tggctgcccc
       61 ccatgctgca agggtggctc cggcggtaaa gaatgatccg gcaatggcgg cagcccccag
      121 gacaatctgg aatacgccac ctgacttggc cccggcgact ctgggaacaa tatgaatcac
      181 agcgccatca ggcagagtct catgtaactg cgccgttaac ccggacgtgc tgacgtcccg
      241 cccggcaatc cgtacctgat accagccgtc gctcagtttc t
//
LOCUS       DBANUU010000569          281 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.denovo.580, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000569 DBANUU010000000
VERSION     DBANUU010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.denovo.580"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            <1..>281
                     /locus_tag="ACIE68_005260"
     CDS             <1..>281
                     /locus_tag="ACIE68_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFX5911366.1"
                     /translation="ETGEMLWEHETGHAYSEQGLATRQEPDGLPPVEWLTYGSGYLAG
                     MKLGGTPLVEYTRDRLHRETARSFGGAGSTAGYEQATAYTLTGQLQSRH"
BASE COUNT           55 a           76 c          116 g           34 t
ORIGIN      
        1 cggagacagg ggagatgctg tgggagcatg agacggggca tgcgtacagc gaacaggggc
       61 tggcgacccg tcaggagccg gacggtctgc cgccggtgga gtggctgacg tatggcagcg
      121 gttatcttgc ggggatgaag ctgggcggaa cgccgctggt cgagtacacg cgggaccggc
      181 tgcaccgcga gacggcccgt agcttcggcg gggcgggcag tacagcgggg tacgaacagg
      241 ccacggcgta caccctcaca gggcagctac agagcaggca c
//
LOCUS       DBANUU010000570        13966 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.guided.116, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000570 DBANUU010000000
VERSION     DBANUU010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.guided.116"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            263..922
                     /locus_tag="ACIE68_005261"
     CDS             263..922
                     /locus_tag="ACIE68_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFX5911367.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1123..1500)
                     /locus_tag="ACIE68_005262"
     CDS             complement(1123..1500)
                     /locus_tag="ACIE68_005262"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFX5911368.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1567..4473)
                     /locus_tag="ACIE68_005263"
     CDS             complement(1567..4473)
                     /locus_tag="ACIE68_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004726714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFX5911369.1"
                     /translation="MPDDLIRHYTFNDTDLSIIRQRRGPANRLGFAVQLCYLRFPGVI
                     LGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRREHLSELQTVFGFRPFTMSHYR
                     QAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALNAVERASAEAITRANRRIYDA
                     LAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKPNSRHMLEHIERLKAWQALDL
                     PTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYATLVALATEGMATVTDEIIDL
                     HDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIGQALIDAKQSGRDAFAAIEAV
                     MSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRYAPEFLAVLKLRAAPAAKNVL
                     DAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDAGIDRRYYELCALSELKNSLR
                     SGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAVATDCEQYLHERLTLLEAQLA
                     TVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALIDQTAMVLPHVKITELLLEVD
                     EWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLTKMAESCPGTTYAKLAWLQAW
                     HTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQNFRTASKAKSTGHINPKYGS
                     SPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLYHESDLRIEEHYTDTAGFTDH
                     VFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKPMIGGTLNIKHVRAHWDEILR
                     LATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRVH
                     AGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVYLERAA
                     HALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
     gene            4532..5236
                     /locus_tag="ACIE68_005264"
     CDS             4532..5236
                     /locus_tag="ACIE68_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFX5911370.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     gene            complement(5364..5924)
                     /locus_tag="ACIE68_005265"
     CDS             complement(5364..5924)
                     /locus_tag="ACIE68_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFX5911371.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(6050..6400)
                     /locus_tag="ACIE68_005266"
                     /pseudo
     CDS             complement(6050..6400)
                     /locus_tag="ACIE68_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(6603..7616)
                     /gene="intI1"
                     /locus_tag="ACIE68_005267"
     CDS             complement(6603..7616)
                     /gene="intI1"
                     /locus_tag="ACIE68_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFX5911372.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            7775..8248
                     /gene="dfrA14"
                     /locus_tag="ACIE68_005268"
     CDS             7775..8248
                     /gene="dfrA14"
                     /locus_tag="ACIE68_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HFX5911373.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            8432..8779
                     /locus_tag="ACIE68_005269"
     CDS             8432..8779
                     /locus_tag="ACIE68_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFX5911374.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            8773..9612
                     /gene="sul1"
                     /locus_tag="ACIE68_005270"
     CDS             8773..9612
                     /gene="sul1"
                     /locus_tag="ACIE68_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFX5911375.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            9740..10240
                     /locus_tag="ACIE68_005271"
     CDS             9740..10240
                     /locus_tag="ACIE68_005271"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFX5911376.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
     gene            complement(10416..11201)
                     /gene="istB"
                     /locus_tag="ACIE68_005272"
     CDS             complement(10416..11201)
                     /gene="istB"
                     /locus_tag="ACIE68_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS1326 family helper ATPase
                     IstB"
                     /protein_id="HFX5911377.1"
                     /translation="MMQHEGHVRILKSLKLFGMAHAIEELGNQNSPAFNQALPMLDSL
                     IKAEVAEREVRSVNYQLRVAKFPVYRDLVGFDFSQSLVNEATVKQLHRCDFMEQAQNV
                     VLIGGPGTGKTHLATAIGTQAVMHLNRRVRFFSTVDLVNALEQEKSSGRQGQIANRLL
                     YADLVILDELGYLPFSQTGGALLFHLLSKLYEKTSVILTTNLSFSEWSRVFGDEKMTT
                     ALLDRLTHHCHILETGNESYRFKHSSTQNKQEEKQTRKLKIET"
     gene            complement(11188..12711)
                     /gene="istA"
                     /locus_tag="ACIE68_005273"
     CDS             complement(11188..12711)
                     /gene="istA"
                     /locus_tag="ACIE68_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS1326 family transposase"
                     /protein_id="HFX5911378.1"
                     /translation="MINVAILSAIRRWHFRDGASIREIARRSGLSRNTVRKYLQSKVV
                     EPQYPARDSVGKLSPFEPKLRQWLSTEHKKTKKLRRNLRSMYRDLVALGFTGSYDRVC
                     AFARQWKDSEQFKAQTSGKGCFIPLRFACGEAFQFDWSEDFARIAGKQVKLQIAQFKL
                     AHSRAFVLRAYYQQKHEMLFDAHWHAFQIFGGIPKRGIYDNMKTAVDSVGRGKERRVN
                     QRFTAMVSHYLFDAQFCNPASGWEKGQIEKNVQDSRQRLWQGAPDFQSLADLNVWLEH
                     RCKALWSELRHPELDQTVQEAFADEQGELMALPNAFDAFVEQTKRVTSTCLVHHEGNR
                     YSVPASYANRAISLRIYADKLVMAAEGQHIAEHPRLFGSGHARRGHTQYDWHHYLSVL
                     QKKPGALRNGAPFAELPPAFKKLQSILLQRPGGDRDMVEILALVLHHDEGAVLSAVEL
                     ALECGKPSKEHVLNLLGRLTEEPPPKPIPIPKGLRLTLEPQANVNRYDSLRRAHDAA"
     gene            complement(12813..13673)
                     /locus_tag="ACIE68_005274"
     CDS             complement(12813..13673)
                     /locus_tag="ACIE68_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HFX5911379.1"
                     /translation="MDEYPIIDLSHLLPAAQGLARLPADERIQRLRADRWIGYPRAVE
                     ALNRLEALYAWPNKQRMPNLLLVGPTNNGKSMIVEKFRRTHPASSDADQEHIPVLVVQ
                     MPSEPSVIRFYVALLAAMGAPLRPRPRLPEMEQLALALLRKVGVRMLVIDELHNVLAG
                     NSVNRREFLNLLRFLGNELRIPLVGVGTRDAYLAIRSDDQLENRFEPMMLPVWEANDD
                     CCSLLASFAASLPLRRPSPIATLDMARYLLTRSEGTIGELAHLLMAAAIVAVESGEEA
                     INHRTLSMAC"
     gene            complement(13676..>13966)
                     /locus_tag="ACIE68_005275"
     CDS             complement(13676..>13966)
                     /locus_tag="ACIE68_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911380.1"
                     /translation="AAYASEINRLKALAKLRQQGREQVDESALFRMIGQMREIVTSAQ
                     KATRKARRDADRRQHLKTSARPDKPVPPDTDIADPQADNLPPAKPFDQIEEW"
BASE COUNT         2890 a         3915 c         4043 g         3118 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1321 cttatttcga tcgcactgag aaacatacag ctctttaata tgcagctgac cgctgtatcg
     1381 gggcgaggga taaagaatat tgcaacatgc caggccagta atgttattgc cgcagcgcgc
     1441 tctgatcacc agggtaccgg aaagccggtt gaataacttt ttacagagat aatccttcat
     1501 caacgcttcc tgaatgatac cttcgccata ataggggtcg tctcagaaaa cggaaaataa
     1561 agcacgctaa gccggttgca gaggccgtag cggcctgaac ttccccgcgc cgatcttggc
     1621 gctgctgcgc cataggtaat caccggtcag gttgatgtgc tcccagccga gtggcgacag
     1681 gtactgcaat agcgagtcat cgacggcatg accattgccg cgcaacgcat gcgccgcacg
     1741 ctccaggtag accgtgttcc acagcacgat ggccgccgtc accaggttga ggccgctggc
     1801 ccggtagcgc tgctgctcga aactgcggtc acggatttca ccaaggcggt tgaagaacac
     1861 ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc ccggcatgca cgcggcggcg
     1921 tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg cgctcgatgc ggcccaactc
     1981 gcgcagcgcg acggccaagc cgttctggcg cgggtagctg ccgagtttcc tgagcatcag
     2041 cgaggccgtc accgtgccct gcttgatcga ggtggccagc cgcaggattt cgtcccaatg
     2101 ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc ggcttgagcg cgtcataggc
     2161 ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg tcgcggatgc gcggcgcgaa
     2221 gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg gtgaagcccg ccgtgtcggt
     2281 gtagtgctcc tcgatccgca ggtcggattc gtggtacagc aggccgtcga gcacgtaggt
     2341 tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat ggcgcgtatt ggtcggagat
     2401 gtgggtgtag aaagtccgtc ctgggctgct gccatatttt gggttgatgt gccccgtgct
     2461 ctttgccttg ctagcggttc ggaaattctg tccgtccgat gatgatgtgg tgccatcgcc
     2521 ccagtgcccg gcaaagggat gccgaaactg agcgttgacc agttcagcca acgctgtcga
     2581 gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg agcttcgcgt aggtcgtgcc
     2641 ggggcaggac tcggccatct tggtcaggcc caggttgatc gcgtcggcca ggatcgtggt
     2701 caacaacagg ttcttgtcct tggccagatc gcccgatttc aagtgcgtga agtgccgggt
     2761 gaagcccgtc cactcatcga cttcgagcag cagttcggtg atcttgacgt gcggcaggac
     2821 catggctgtc tggtctatca gcgcctgcgc ggtgtcgggc accgccgcat ccagcggcgt
     2881 gatcttcaag cccgactcgg tgatgatggc atccggcagg tcgttggctg ccgccatgcg
     2941 gttgacggtg gcaagttgtg cttccagcag cgtcagccgc tcatgcagat attgttcgca
     3001 gtcggtggcc acggccagcg gcaattcgct ggactgcttg aggctggtga acttctcggg
     3061 cggtaccagg tagtcctcga agtccttgaa ctggcgtgaa ccctgcaccc agatgtcgcc
     3121 cgagcgcagg gagttcttca actcggacag cgcgcacagt tcgtagtagc gccggtcgat
     3181 gccggcgtcg gtcatcacca gtttctgcca gcgcggcttg atgaagccgg tcggtgcatc
     3241 ggctggcagc ttgcgggcgt tgtcggtgtt catgccgcgc agcacctcaa tggcatcaag
     3301 cacgtttttg gcggcgggcg cggcccgcag cttgagcacg gcaaggaatt ccggtgcata
     3361 gcggcgcagg gtggcgtagc tctcgccgat gcgatgcagg aaatcgaagt catcgggttg
     3421 cgcgagcttc tgcgcctcgg tgacgctctc ggcaaaggaa tcccaggaca tgacggcctc
     3481 gatggcggca aacgcatcgc ggcctgattg cttggcgtcg atcagcgcct gaccgatgcg
     3541 cccgtacaga cgtaccttgg cgttgatggc cttgcctgac gcctggaact gctgctgatg
     3601 cttattcttg gcagcgttaa acagcttacc caggatgcgg tcgtgcaggt cgatgatttc
     3661 gtcggtgacg gtggccatgc cctcggtggc cagcgccacg agagtggcgt agcgccgttg
     3721 cggctcgaat ttggccaggt cggcgggtgt catctggccg ccctcgcggg caatcttgag
     3781 caggcggttc tggtgaacca gccgctcgat gccggtaggc agatcgagtg cctgccatgc
     3841 cttgaggcgt tcgatgtgtt ccagcatatg ccgcgaattt ggcttggccg gagactggcg
     3901 caaccaagcc aaccaggtcg tcttgccgtt gtcccggcgc ttgagcagat cgtcgaggcg
     3961 gcggcgatgc gcgtccgcca gtggttcggc caaggcgtcg tagatgcgcc ggttagcacg
     4021 ggtgatcgcc tcggcactcg cccgctcgac ggcgttgagg gcgggcagaa tgaccgactg
     4081 ccgccgcagg tgcccgatca aggcgctggc cagcacgatg cctttgtcgg tttgcatcgc
     4141 cagctcggtc agcatctgga cggcctgccg gtaatggctc atggtgaagg gccggaaacc
     4201 gaacacggtt tgcagctcgc tcaggtgctc gcgccgggtc tgctcccgct ggccgtactc
     4261 gttccagctt tcgacgccga ccttgagctg gtcggcgacc agcttcaaca agggcgggaa
     4321 cggtagttca tcgacgccca ggatgacgcc gggaaagcgc aggtaacaga gctgcaccgc
     4381 gaagcccagc cgattggctg gcccgcgccg ctgtcggatg atcgagaggt cggtatcgtt
     4441 gaatgtgtaa tgtcggatca ggtcgtccgg cactgttgca aatagtcggt ggtgataaac
     4501 ttatcatccc cttttgctga tggagctgca catgaaccca ttcaaaggcc ggcattttca
     4561 gcgtgacatc attctgtggg ccgtacgctg gtactgcaaa tacggcatca gttaccgtga
     4621 gctgcaggag atgctggctg aacgcggagt gaatgtcgat cactccacga tttaccgctg
     4681 ggttcagcgt tatgcgcctg aaatggaaaa acggctgcgc tggtactggc gtaacccttc
     4741 cgatctttgc ccgtggcaca tggatgaaac ctacgtgaag gtcaatggcc gctgggcgta
     4801 tctgtaccgg gccgtcgaca gccggggccg cactgtcgat ttttatctct cctcccgtcg
     4861 taacagcaaa gctgcatacc ggtttctggg taaaatcctc aacaacgtga agaagtggca
     4921 gatcccgcga ttcatcaaca cggataaagc gcccgcctat ggtcgcgcgc ttgctctgct
     4981 caaacgcgaa ggccggtgcc cgtctgacgt tgaacaccga cagattaagt accggaacaa
     5041 cgtgattgaa tgcgatcatg gcaaactgaa acggataatc ggcgccacgc tgggatttaa
     5101 atccatgaag acggcttacg ccaccatcaa aggtattgag gtgatgcgtg cactacgcaa
     5161 aggccaggcc tcagcatttt attatggtga tcccctgggc gaaatgcgcc tggtaagcag
     5221 agtttttgaa atgtaaggcc tttgaataag acaaaaggct gcctcatcgc taactttgca
     5281 acagtgccgg tcgtccttgg agtccggcaa cgccagcagg ctttcccgct cggcggcgga
     5341 caggatggaa cgacgtggca tatttactga tccgttctca agtattgata cagggtttcg
     5401 cgactgattc cgaattcacg agcaagcttg gtcttttgct cgccagcctc gacacgttgg
     5461 cgcagttcgg caatacgctc agacgacagg gatttcttcc tgccacggta agccccgcgt
     5521 tgcttggcga gcgcaatacc ctcgcgctga cgctcgcgga tcagggcgcg ctcgaactcg
     5581 gcgaacgcgc ccatcaccga gagcatcagg ttcgccatcg gagagtcttc gccagtaaaa
     5641 ctgaggtgtt ccttgacgaa ttcgatatgc acgccgcgtt gtgtcagcgt ttgcacgatc
     5701 cggcgcaaat catcgagatt gcgcgccagg cgatccatgc tatgcaccac cacggtgtcg
     5761 ccggtgcggg cgaagcttat cagcgcttcc agttgcggac gcttgacatc cttgccggat
     5821 gccttgtcgc taaaagcgcg atcaaccttg acgccttcca gttgccgttc cgggttctgg
     5881 tcgaaggtgc tgaccctgat atacccaatg cgctgtccag tcatggaatt ccctgcaaaa
     5941 tgtcagggaa gactctatga ccttcaacga gatatgtcaa taaattcaaa attcaatcct
     6001 atcctgacgc aatttacaca tggcatctga catcaggtta gggtatgcct caacctgacg
     6061 gcggcgaatc acaagcgtcc ggtttgacgc tggtttcggt cgcagtgctg gcccgcgcct
     6121 ggaagcgctg atagcactcc agcccgcaga aatgctccac gtactcggcc ccttccggcg
     6181 tgaaggcggc atcgagcggg atttccttgc agcacacgca gcaactggtg ctcggttcat
     6241 tggcgttcat ggtggtgttc ctccattggt tgacgaagcc gacgaaggcc gccgccggca
     6301 tcggcctggc gaacaggaaa ccctgcaccg tgtcgcaacc cgcctgccgc aaccacgcaa
     6361 ggcagtcggg tgtttccaca ccctcggcta ccacctccat tgtcgttttc agaagacggc
     6421 tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat ccatcaggca
     6481 acgacgggct gctgccggcc atcagcggac gcagggagga ctttccgcaa ccggccgttc
     6541 gatgcggcac cgatggcctt cgcgcagggg tagtgaatcc gccaggattg acttgcgctg
     6601 ccctacctct cactagtgag gggcggcagc gcatcaagcg gtgagcgcac tccggcaccg
     6661 ccaactttca gcacatgcgt gtaaatcatc gtcgtagaga cgtcggaatg gccgagcaga
     6721 tcctgcacgg ttcgaatgtc gtaaccgctg cggagcaagg ccgtcgcgaa cgagtggcgg
     6781 agggtgtgcg gtgtggcggg cttcgtgatg cctgcttgtt ctacggcacg tttgaaggcg
     6841 cgctgaaagg tctggtcata catgtgatgg cgacgcacga caccgctccg tggatcggtc
     6901 gaatgcgtgt gctgcgcaaa aacccagaac cacggccagg aatgcccggc gcgcggatac
     6961 ttccgctcaa gggcgtcggg aagcgcaacg ccgctgcggc cctcggcctg gtccttcagc
     7021 caccatgccc gtgcacgcga cagctgctcg cgcaggctgg gtgccaagct ctcgggtaac
     7081 atcaaggccc gatccttgga gcccttgccc tcccgcacga tgatcgtgcc gtgatcgaaa
     7141 tccagatcct tgacccgcag ttgcaaaccc tcactgatcc gcatgcccgt tccatacaga
     7201 agctgggcga acaaacgatg ctcgccttcc agaaaaccga ggatgcgaac cacttcatcc
     7261 ggggtcagca ccaccggcaa gcgccgcgac ggccgaggtc ttccgatctc ctgaagccag
     7321 ggcagatccg tgcacagcac cttgccgtag aagaacagca aggccgccaa tgcctgacga
     7381 tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg acagaaatgc ctcgacttcg
     7441 ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag
     7501 tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac
     7561 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     7621 tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt
     7681 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt
     7741 cgccctaaaa caaagttaac ccaggatgag aaccttgaaa gtatcattga tagctgcgaa
     7801 agcgaaaaac ggcgtgattg gttgcggtcc agacataccc tggtccgcga aaggggagca
     7861 gctacttttt aaagcattga cctacaatca gtggcttctg gtgggtcgca agacgtttga
     7921 atctatgggc gcactcccca ataggaaata cgcggtcgtt acccgctcag gttggacatc
     7981 aaatgatgac aatgtagttg tatttcagtc aatcgaagag gccatggaca ggctagctga
     8041 attcaccggt cacgttatag tgtctggtgg cggagaaatt taccgagaaa cattacccat
     8101 ggcctctacg ctccacttat cgacgatcga catcgagcca gagggggatg ttttcttccc
     8161 gagtattcca aataccttcg aagttgtttt tgagcaacac tttacttcaa acattaacta
     8221 ttgctatcaa atttggaaaa agggttaaca aagctatgca atcgacggca aaaagcttcg
     8281 ttcgcttcgc gcactacgcc tttttccgcg attgatagcg acgttagatg cactaagcac
     8341 ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt aagcgtgcat
     8401 aataagccct acacaaattg ggagatatat catgaaaggc tggctttttc ttgttatcgc
     8461 aatagttggc gaagtaatcg caacatccgc attaaaatct agcgagggct ttactaagct
     8521 tgccccttcc gccgttgtca taatcggtta tggcatcgca ttttattttc tttctctggt
     8581 tctgaaatcc atccctgtcg gtgttgctta tgcagtctgg tcgggactcg gcgtcgtcat
     8641 aattacagcc attgcctggt tgcttcatgg gcaaaagctt gatgcgtggg gctttgtagg
     8701 tatggggctc ataattgctg cctttttgct cgcccgatcc ccatcgtgga agtcgctgcg
     8761 gaggccgacg ccatggtgac ggtgttcggc attctgaatc tcaccgagga ctccttcttc
     8821 gatgagagcc ggcggctaga ccccgccggc gctgtcaccg cggcgatcga aatgctgcga
     8881 gtcggatcag acgtcgtgga tgtcggaccg gccgccagcc atccggacgc gaggcctgta
     8941 tcgccggccg atgagatcag acgtattgcg ccgctcttag acgccctgtc cgatcagatg
     9001 caccgtgttt caatcgacag cttccaaccg gaaacccagc gctatgcgct caagcgcggc
     9061 gtgggctacc tgaacgatat ccaaggattt cctgaccctg cgctctatcc cgatattgct
     9121 gaggcggact gcaggctggt ggttatgcac tcagcgcagc gggatggcat cgccacccgc
     9181 accggtcacc ttcgacccga agacgcgctc gacgagattg tgcggttctt cgaggcgcgg
     9241 gtttccgcct tgcgacggag cggggtcgct gccgaccggc tcatcctcga tccggggatg
     9301 ggatttttct tgagccccgc accggaaaca tcgctgcacg tgctgtcgaa ccttcaaaag
     9361 ctgaagtcgg cgttggggct tccgctattg gtctcggtgt cgcggaaatc cttcttgggc
     9421 gccaccgttg gccttcctgt aaaggatctg ggtccagcga gccttgcggc ggaacttcac
     9481 gcgatcggca atggcgctga ctacgtccgc acccacgcgc ctggagatct gcgaagcgca
     9541 atcaccttct cggaaaccct cgcgaaattt cgcagtcgcg acgccagaga ccgagggtta
     9601 gatcatgcct agcattcacc ttccggccgc ccgctagcgg accctggtca ggttccgcga
     9661 aggtgggcgc agacatgctg ggctcgtcag gatcaaactg cactatgagg cggcggttca
     9721 taccgcgcca ggggagcgaa tggacagcga ggagcctccg aacgttcggg tcgcctgctc
     9781 gggtgatatc gacgaggttg tgcggctgat gcacgacgct gcggcgtgga tgtccgccaa
     9841 gggaacgccc gcctgggacg tcgcgcggat cgaccggaca ttcgcggaga ccttcgtcct
     9901 gagatccgag ctcctagtcg cgagttgcag cgacggcatc gtcggctgtt gcaccttgtc
     9961 ggccgaggat cccgagttct ggcccgacgc cctcaagggg gaggccgcat atctgcacaa
    10021 gctcgcggtg cgacggacac atgcgggccg gggtgtcagc tccgcgctga tcgaggcttg
    10081 ccgccatgcc gcgcgaacgc aggggtgcgc caagctgcgg ctcgactgcc acccgaacct
    10141 gcgtggccta tacgagcggc tcggattcac ccacgtcgac actttcaatc ccggctggga
    10201 tccaaccttc atcgcagaac gcctagaact cgaaatctaa cgtccgttcg ggcatcgagg
    10261 tccatgtcgg ggtgggacgg gcccgtggct tcaagatcac ttgcagtccg accgcgatgt
    10321 cttggttgcg cgagaggttg tcgatatctg ttgatttgca cccaaatttg acccgggatt
    10381 tgcattgaat tttgacccac cccttgttgt cagaattatg tctcgatttt cagtttgcgg
    10441 gtctgttttt cctcctgctt attctgagtt gaactgtgtt tgaagcggta actttcattg
    10501 ccggtttcca ggatgtggca gtggtgggtt agtcggtcca acaacgctgt tgtcatcttt
    10561 tcatcgccaa acactcggct ccattccgag aagctcaagt tggtggtcag tatcacgctg
    10621 gttttttcgt acagctttga gagcaggtga aacagcagtg ccccaccggt ttggctaaaa
    10681 ggcaaatatc ccagctcatc cagaatcacc aaatcggcat acaacagacg gtttgcgatt
    10741 tgtccctgac gcccagatga tttctcttgc tccagtgcat tgaccaaatc cacggtggag
    10801 aagaaacgca cccgtcggtt caagtgcatc actgcttgtg taccaatggc tgtggccagg
    10861 tgagtcttgc ctgtgcctgg cccaccaatc agcaccacgt tctgggcttg ttccatgaag
    10921 tcgcaccggt gcaattgttt gaccgtggcc tcattaacca ggctttgact gaagtcaaag
    10981 cccaccaagt cccgatacac ggggaacttg gccacccgca attgatagtt caccgaacgt
    11041 acttcacgct ctgccacttc agctttaatc aagctgtcca gcatgggcaa ggcttgatta
    11101 aatgctggtg aattctgatt gcccaactcc tcaatggcgt gtgccatgcc aaagagtttc
    11161 aaggatttga ggattctcac atggccttca tgctgcatca tgggctctcc ttaaactgtc
    11221 atagcggttc acgttggcct gtggttccaa tgtcagcctt aaccccttgg gaattggaat
    11281 cggtttgggt ggaggttctt cggtcaaacg tcccaacaga ttaagcacat gctccttcga
    11341 tggcttgcca cactccaatg ccaattccac agcactgagt accgcacctt catcgtggtg
    11401 caatacaagg gccagaattt ccaccatgtc acggtcaccg ccggggcgtt gcagcaagat
    11461 ggattgaagc ttcttgaacg cgggtggcaa ttcagcaaat ggcgcaccat tgcgcaacgc
    11521 cccaggtttc ttctgaagca cagacaagta atggtgccag tcgtattgtg tgtggccacg
    11581 ccgagcgtgg ccactgccaa acaatcttgg atgctcggca atgtgttggc cttcggcagc
    11641 catcaccagc ttgtctgcat aaatccgaag gctgatggcc ctgttggcgt aactggcagg
    11701 aacgctgtag cgattgccct cgtggtgaac aaggcaggtt gaagtgactc gcttggtttg
    11761 ctccacgaat gcatcaaagg cattgggtag cgccatcaac tcgccttgtt catcggcaaa
    11821 ggcctcttgc acggtttggt ccaattcggg gtggcgcagc tcagaccaca gcgctttgca
    11881 gcgatgctca agccacacat tcaaatcagc aaggctttga aagtctggtg ccccttgcca
    11941 caggcgttgg cgggaatcct gcacgttctt ctcaatctgg cctttctccc aacccgatgc
    12001 tggattacag aactgcgcat caaacaggta gtggctgacc atggcagtga accgctgatt
    12061 gaccctgcgc tctttgccac gccccaccga atccacagcg gtcttcatgt tgtcgtagat
    12121 gccgcgcttg ggaatgccac cgaagatttg aaaggcatgc cagtgggcat caaacagcat
    12181 ttcatgtttt tgctggtagt aagcccgaag cacaaaggcc cggctgtggg ccaacttaaa
    12241 ctgggcaatc tgaagtttga cctgtttgcc cgctatgcgg gcaaagtcct cactccaatc
    12301 gaattggaag gcttcgccac aagcaaagcg caaggggatg aaacaaccct tgcccgaggt
    12361 ttgcgccttg aactgttcgg aatctttcca ctgtcgggca aaggcacaca ctcggtcata
    12421 agacccggta aagcccaaag cgaccaaatc ccggtacatg ctgcgcaggt ttctgcgcag
    12481 cttctttgtc tttttgtgct cggtggagag ccactgcctt aacttgggct caaaaggact
    12541 taacttgcca acgctgtctc gcgctgggta ctgcggttca accaccttgc tttgcaaata
    12601 cttgcgaacg gtgttcctgg acaggccgct tcgtcgggct atttcccgaa tcgacgcacc
    12661 atcgcgaaaa tgccagcgtc gaattgcgct caatatcgcc acgtttatca ctccttgatt
    12721 tctcccgcca tatccagacg ggaaacagtg tcatacgtgg gtcaaatttc gacgcaaatc
    12781 tttaccctaa gtgggtcaat tttagatgca actcaacagg ccatgctgag tgtgcgatgg
    12841 ttgatcgctt cctcgccgct ctccacggcg acgatggccg ccgccatcag caagtgcgcc
    12901 agttccccta tggtgccctc gctgcgtgtg agcaggtagc gagccatgtc cagcgtggca
    12961 attggggaag gccggcgcag cgggagcgaa gcggcgaagc tggccagcag tgagcagcaa
    13021 tcgtcgttgg cctcccatac cggcagcatc atcggctcga agcgattttc caactggtca
    13081 tcggagcgga tggctaggta ggcgtcgcgc gtgcctaccc caaccaacgg gatgcgcagt
    13141 tcgttgccga ggaagcgcag caggttgagg aattcccggc ggttgacgct gttgccggcc
    13201 agcacgttgt gcagctcgtc gatcaccagc atgcgcacgc cgaccttgcg cagcagtgcc
    13261 agagccagtt gctccatttc cggcaaccgt gggcgtgggc gcagcggcgc gcccatcgcg
    13321 gcgagcagcg cgacgtagaa gcggatcacg gacggctcgg acggcatctg cacgaccaac
    13381 accgggatgt gctcctggtc ggcgtcggag ctggccgggt gggtgcggcg gaacttctcg
    13441 acgatcatcg acttgccatt gttggtcggg ccaaccagca gcaggttggg catgcgttgc
    13501 ttgtttggcc acgcataaag ggcttccagc cggttcagcg cctcgactgc gcgcggatag
    13561 ccgatccagc ggtcggcgcg aaggcgctgg atgcgctcgt ccgccggaag acgggccaag
    13621 ccctgggccg ccggcagcag gtgggacagg tcgatgatgg gatattcgtc cacggctacc
    13681 actcctcaat ctggtcgaac ggtttggcgg gtggcaagtt gtctgcctgc gggtcggcaa
    13741 tatccgtatc cggcggaacg ggcttgtccg gccgagctga tgtcttgagg tgctggcggc
    13801 gatccgcgtc acgccgcgcc ttgcgtgtgg ccttctgcgc gctggtcaca atctcacgca
    13861 tctggccgat catgcggaac agcgccgact catccacctg ttcgcgccct tgctgccgca
    13921 gtttcgccag cgccttcagc cggttgattt cggaagcata agccgc
//
LOCUS       DBANUU010000571        10711 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.guided.148, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000571 DBANUU010000000
VERSION     DBANUU010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.guided.148"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            366..572
                     /locus_tag="ACIE68_005276"
                     /pseudo
     CDS             366..572
                     /locus_tag="ACIE68_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            568..920
                     /gene="prfH"
                     /locus_tag="ACIE68_005277"
                     /pseudo
     CDS             568..920
                     /gene="prfH"
                     /locus_tag="ACIE68_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(977..2434)
                     /gene="pepD"
                     /locus_tag="ACIE68_005278"
     CDS             complement(977..2434)
                     /gene="pepD"
                     /locus_tag="ACIE68_005278"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFX5911381.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQDILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIKSVGHYWTLLTELLKEIPAK"
     gene            2695..3153
                     /gene="gpt"
                     /locus_tag="ACIE68_005279"
     CDS             2695..3153
                     /gene="gpt"
                     /locus_tag="ACIE68_005279"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFX5911382.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDNYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3245..4489
                     /gene="frsA"
                     /locus_tag="ACIE68_005280"
     CDS             3245..4489
                     /gene="frsA"
                     /locus_tag="ACIE68_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFX5911383.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4547..4948
                     /gene="crl"
                     /locus_tag="ACIE68_005281"
     CDS             4547..4948
                     /gene="crl"
                     /locus_tag="ACIE68_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFX5911384.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(4987..6042)
                     /gene="phoE"
                     /locus_tag="ACIE68_005282"
     CDS             complement(4987..6042)
                     /gene="phoE"
                     /locus_tag="ACIE68_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFX5911385.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6330..7433
                     /gene="proB"
                     /locus_tag="ACIE68_005283"
     CDS             6330..7433
                     /gene="proB"
                     /locus_tag="ACIE68_005283"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFX5911386.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7445..8698
                     /gene="proA"
                     /locus_tag="ACIE68_005284"
     CDS             7445..8698
                     /gene="proA"
                     /locus_tag="ACIE68_005284"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFX5911387.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPVGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MEESGVALHADAAALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            8813..8888
                     /locus_tag="ACIE68_005285"
     tRNA            8813..8888
                     /locus_tag="ACIE68_005285"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8846..8848,aa:Thr,seq:cgt)
     gene            9054..10268
                     /locus_tag="ACIE68_005286"
     CDS             9054..10268
                     /locus_tag="ACIE68_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFX5911388.1"
                     /translation="MKLNARQIDTAKPKEKAYKLADGGGLYLLVKPNGGKYWRLKYRV
                     AGKEKLLALGVYPEVTLADARAKREDAKRGIAGGIDPMEAKREEKIARETQLNNTFKD
                     IALEWHSSKLKKWSAGYASDILEAFNKDVFPYIGKKPIAEIKPLELLNVLRRIEGRGA
                     TEKAKKVRQRCGEVFRYAIVTGRAEYNPAPDLTSAMQGHESNHYPFLTAKELPDFFKA
                     LSSYSGSALVVMAARLLIITGLRTGELRGALWDEIDFNKAIWEIPASRMKMRRPHIVP
                     LSEQALSLIGKIREITGNYPLMFPGRNDPRKTMSEASINQVFKRIGYAGRVTGHGFRH
                     TMSTILHEQGYNTAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLEHG
                     GNVVHMVFEKHA"
BASE COUNT         2695 a         2577 c         2760 g         2679 t
ORIGIN      
        1 gagcgtggtt tactaatttt tatatgcgat ataaaccgca acattaaatc cagcttgcaa
       61 tgaaaataac gcccgcctgg tatgtgcgcg ttttcttatt ccacaaactg caaggaggta
      121 aatcatgaca aatcctttat caatgactct ttgcagacct ttccaggatt aattcttttt
      181 ttcttgccct ggattcgtct gccatttcct gatttttaat atttatatac tctaaataat
      241 tcgagttgca ggaaggtgac aagcgagtga atcgccagga gcttactaaa gtaagtgact
      301 ggggtgaacg aacgcagtcg cagtacatgc aacttgaagt atgacgagta taaatggaat
      361 ggtacatggg caaatatatt cgtcccttat ccgatgcggt atttaccatc gcatctgatg
      421 acctgtggat cgagagttta gcgatccaac aattacacac cacggcaaat ttacccaaca
      481 tgcagcgcgt agtggggatg ccagatttac atcccggacg cggctatccg attggcgcag
      541 cgttcttctc tgttggtcgt ttttatccgg caagacgtcg cggtaacggt gctggaaaca
      601 gaaacgggcc gctactctga cacgctgcgt tcggcgctgg tttctctgga tggcgataac
      661 gcatgggcat taagcgaaag ctggtgcggc actattcagt ggatttgtcc gagtccgtat
      721 cggtctcatc atgggcgcaa aaactggttt ctgggcatgc taacaaacgg ctggcgcgat
      781 tgctgattgc ctggaagctg gagcagcagc aacaggaaaa tagcgcggcg ctgaaatcgc
      841 agcggcgaat gttccatcac cagattgaac gtggcaaccc gcgacggaca tttacaggga
      901 tggcttttat cgaaggataa tgaaggatga aactgccgga aggcgattaa acgccatccg
      961 gcagcaaatc aaataattac ttcgccggaa tttctttcag cagttcagtc agcagtgtcc
     1021 agtaatgacc tacgcttttg atgtgaactt gctcatccgg agagtgtgga ccggtgatag
     1081 ttggcccgat agaaaccatg tccatttccg gatacggttt tttaaacaga ccacattcca
     1141 ggcccgcgtg gataatctgg atgttcggcg tcttgttgaa caggcgctga taggtttcac
     1201 gtaccagatg catcaccgga gaattagcgt ccggctgcca gccaggatat gcgcctttcg
     1261 cttcggtttt cgcgccagcc agtttaccca gcgaatccag catgctcacc acgtagtctt
     1321 taccgctgtc gatcagtgaa cggatcaggc agtgaatttc tacgttattg tcagtcatgg
     1381 tcaccacacc gacgttcagg gaggtttcga ccacaccttt tgccacgtcg gaattgcgga
     1441 tcacaccgtt cggggtggcg ttcagcagac gaataaaggt atcgcgagat ttcgcaatca
     1501 gagcagcttt atcgttcgct acagagtcca gcaacaaggc cagattcttc tctttctctg
     1561 ccagctcgtt tttcaggatg tcctgatagg tattcaccag agatttcagg gcgtcgactt
     1621 tatcagctgc gacagcaatg gtcgcaaagg cttcacgcgg gatggcgtta cgcagtgtgc
     1681 caccgttgaa atcgataagg cgcaggtcca gctcttccgc atgacccgcc aggaagcgca
     1741 ccagcagttt gttggcatta cccaggccaa cgtggatttc accgccggag tgaccgcctt
     1801 ttagaccttt taaggttaac ttgaaggttt caaagccagc tggaaccgct tcacgatcca
     1861 aatgcaggtt ggaggtgaag tcgatacccc ccgcgcaacc catgtagatt tcaccttctt
     1921 cttcggagtc ggtgttaatc agaatatcag cctgcaacca gttgctctgt aagccgaacg
     1981 caccgtccat accggcttct tcggtcatgg tcagcagcac ttccagcggg ccgtgaacca
     2041 cgttttcgtc agccagaacc gccagcgcag acgccatacc aatgccgtta tctgcaccca
     2101 gcgtggtgcc gcgcgcttta acccattcgc catcaatata aggctggata ggatctttcg
     2161 tgaagtcatg cacggtgtcg ttatttttct gcggcaccat atcgaggtgg gcctgcaaga
     2221 cgaccggttt acgattttcc atacctgcgg tagcaggttt acgaatcagg atattaccta
     2281 cctgatcgcg ttcgacatgg aaacctttct cttttgccca accaacaatg tattcagcga
     2341 gttgctcttc atgataggac gggtgaggaa tagaacagat tttggcaaaa atatcccaca
     2401 gcggctgtgg agataattga gacagttcag acacgttaag tctccttgtc gatcacccgc
     2461 aaaacagtat tgcaggtcac agggttagca gaaaatgttg tcaacacaag acaggcttgc
     2521 gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac
     2581 gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct
     2641 attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc
     2701 gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga
     2761 ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctggtaccg
     2821 ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc
     2881 tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa
     2941 ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa
     3001 atgtatccaa aagcgcactt tgtcaccatc tttgcaaaac cggctggtcg tccgctggtt
     3061 gataattatg ttgttgatat cccgcaagat acctggatcg aacagccgtg ggatatgggc
     3121 gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc
     3181 gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc
     3241 atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga
     3301 aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct
     3361 tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg
     3421 cggcattgac ccacgtgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgctga
     3481 acgtaccgat gatgatttat acgataccgt gattggctac cgtggtggca actggattta
     3541 tgagtgggcc acccaggcga tggtgtggca acaaaaagcc tgtgcggaag aagatccgca
     3601 actcagtggt cgacactggc tgcatgcggc tacgttgtac aacattgccg cctatcctca
     3661 tctgaaagga gatgacctgg ccgagcaagc gcaggcttta tcaaaccgcg cctatgaaga
     3721 ggccgcccag cgtctgccgg gcacgatgcg gcagatggag tttaccgtac ccggcggtgc
     3781 gcccatcacc ggctttttgc atatgccgaa aggcgatggc ccgttcccga cagtattaat
     3841 gtgtggtggt ctggatgcga tgcagacgga ctattacagc ctgtatgaac gttattttgc
     3901 gccgcgcggc attgcgatgc tgactattga tatgccgtcg gtgggctttt cttcaaaatg
     3961 gaagctcacc caggactcca gcctgttgca tcagcacgtc ttaaaggcgc tgcctaacgt
     4021 accgtgggtg gatcacactc gcgtcgcggc ctttggtttc cgtttcggcg ctaacgttgc
     4081 cgtgcgtctg gcataccttg aatcgccgcg tctgaaagcg gttgcctgtc ttggtccggt
     4141 agttcatacc ctgttgagtg attttaagtg ccagcaacag gtgccggaaa tgtatcttga
     4201 cgttctggcg agtcgtttgg ggatgcatga tgcttccgat gacgcgttgc gcgtggagct
     4261 gaatcgctat tcattaaaag tgcaaggatt gctgggacgt cgctgcccaa cgccaatgtt
     4321 atcaggctac tggaagaacg atccgttcag cccggaagag gactcacgct taatcacctc
     4381 atcatctgct gacggtaaat tattagagat cccatttaac ccggtgtatc ggaattttga
     4441 caaaggtctt caggaaatca ccggctggat cgaaaaacgc ttgtgttaaa aatttgctaa
     4501 attttgccaa tttggtaaaa cagttgcatc acaacaggag atagcaatga cgttaccgag
     4561 tggacacccg aagagcagat tgatcaaaaa atttaccgca ctaggcccgt atattcgtga
     4621 aggtaagtgc gaagataatc gattcttttt cgattgtctg gctgtatgcg tcaacgtgaa
     4681 accggcaccg gaagtgcgtg aattctgggg ctggtggatg gagctggaag cgcaggaatc
     4741 ccgttttacc tacagctacc agtttggtct gttcgataaa gcaggcgact ggaagagcgt
     4801 tccggtaaaa gacactgaag tggttgaacg actggagcac accctgcgtg agtttcacga
     4861 gaagctgcgt gaactgctga cgacgctgaa tctgaagctg gaaccggcgg atgattttcg
     4921 tgacgagccg gtgaagttaa cggcgtgagt gaaatgtgcc ggatgcatca catccggcaa
     4981 taatcattaa aactgatagg tcatgccaac cgcgacaata tcatcattat taatattcaa
     5041 tttgttatcg ctatccagtt ggttgatttt ataatcaaca aacgctgaca tatttttgtt
     5101 gaaataatat gtagccccga catcgatata attgaccaga tcttcatcac cgataccttc
     5161 aatatctttc cctttcgata agacataacc cagcgatgga cgcagaccaa agtcaaactg
     5221 gtattgagcg accgcttcaa agttctgtgt cttattggca aagccgccag ttattggcgt
     5281 cattttgcgt gtttcagaat aaaaagttgc cagataaata ttattggcat cgtatttcag
     5341 acctgttgcc catgcttctg cacgcttgcc agtgccacgg ctttgcaggt tctgctcgtt
     5401 ggtgcgatct gagttggtat aggcaccact aatggcgaaa tcgctgccgc caaagtcata
     5461 tgtcaatgac gtgccgaagc catcgccgtt ttgcttttta acgtcgcggt tttcgttttt
     5521 cccttgatat tgcagggtta agttcaggcc atcgataacg ccgaagaagt cggtgttccg
     5581 atacgtcgcc agaccgctgg cgcgtttggt cataaagttg tcggtctgcg ccgaggagtc
     5641 gccaccaaat tccgggaaca tatcggtcca ggcttccacg tcatacaacg cgcccaggtt
     5701 acggccatag tcgaaagaac ccaaatcttt atacttcaat ccggcaaaag cgagacgcgt
     5761 tttttgctgt gcagtatcac tctccgcttt atttccggca aactccgctt cccaacggcc
     5821 atagccagtc agttgatcgt taatttgtgt ttcgccttta aaaccaaaac ggatataact
     5881 ctggtcgcca tctttactgt cgttatcact catataatgc atggctttaa ctttgccata
     5941 gacatccagt ttattaccgt ctttgttata tatttctgcg gcctgtacgg atgcagatgc
     6001 cacaatgccc atcaccacta atgccagagt gctctttttc attttcattc ctgattttaa
     6061 ttaacgcgcg aatattcagc gggagagccc cgttgaaaac aggaaagttt ttaacctgag
     6121 attgttaaag atatattaca gattaataat attcttaaaa tgtggtaatt tattaaatct
     6181 gtaataaaag cgtaaacaac tgccgctagg cttgctgatc ccgcgcaaca aaacgccatg
     6241 ctttgcttgc agatggttgg caaccgacga cagtcctgct aaaacgttcg tttgatatca
     6301 tttttcctaa aattgaatgg cagagaatca tgagtgacag ccagacgctg gtggtaaaac
     6361 tcggcaccag tgtgctaaca ggcggatcgc gccgtctgaa ccgtgcccat atcgttgaac
     6421 ttgttcgcca gtgcgcgcag ttacatgccg ccgggcatcg gattgttatt gtgacgtcgg
     6481 gcgcgatcgc cgccggacgt gagcacctgg ggtacccgga actgccagcg actatcgcct
     6541 cgaaacaact gctggcggcg gtagggcaga gtcgactgat tcaactgtgg gaacagctgt
     6601 tttcgattta tggcattcac gtcgggcaga tgctgctgac ccgtgctgat atggaagacc
     6661 gtgaacgctt cctgaacgcc cgcgacaccc tgcgtgcgtt gctcgataac aatatcgttc
     6721 cggtaatcaa tgagaacgat gctgtcgcta cggcagagat taaggtcggc gataacgata
     6781 acctttctgc gctggcggcg attctggcgg gtgccgataa actgttgctg ctgaccgatc
     6841 aaaaaggttt gtataccgct gatccgcgca gcaatccgca ggcagaactg attaaagatg
     6901 tttacggcat tgatgacgca ctgcgcgcga ttgccggtga cagcgtttca ggcctcggaa
     6961 ctggcggcat gagtaccaaa ttgcaggccg ctgacgtggc ttgccgtgcg ggtatcgaca
     7021 ccattattgc cgcgggcagc aagccgggcg ttattggtga tgtgatggaa ggcatttccg
     7081 tcggtacgct gttccatgcc caggcgactc cgcttgaaaa ccgtaaacgc tggattttcg
     7141 gtgcgccgcc ggcgggtgaa atcacggtag atgaaggggc aactgccgcc attcttgaac
     7201 gcggcagctc cctgttgccg aaaggcatta aaagcgtgac tggcaatttc tcgcgtggtg
     7261 aagtcatccg catttgcaac ctcgaaggtc gcgatatcgc ccacggcgtc agtcgttaca
     7321 acagcgatgc attacgccgt attgccgggc accactcgca agaaattgat gcaatactgg
     7381 gatatgaata cggcccggtt gccgttcacc gtgatgacat gattacccgt taaggagcag
     7441 gctgatgctg gaacaaatgg gcattgccgc gaagcaagcc tcgtataaat tagcgcaact
     7501 ctccagccgc gaaaaaaatc gcgtgctgga aaaaatcgcc gatgaactgg aagcacaaag
     7561 cgaaatcatc ctcaacgcta acgcccagga tgttgctgac gcgcgtgcca atggccttgg
     7621 cgaagcgatg cttgaccgtc tggcactgac gcccgcacgg ctgaaaggca ttgccgatga
     7681 tgtgcgccag gtgtgtaacc tcgccgatcc ggtggggcag gtaatcgatg gcagcgtact
     7741 ggacagcggc ctgcgtcttg agcgtcgtcg cgtaccgctg ggggttattg gcgtgattta
     7801 tgaagcgcgc ccgaacgtga cggttgatgt cgcttcgctg tgcctgaaaa ccggtaacgc
     7861 agtgattctg cgtggcggta aagaaacctg tcgcactaac gcggcaacgg tggcggtgat
     7921 tcaggacgcc ctgaaatcct gtggcttacc ggtgggtgcc gtgcaggcga ttgataatcc
     7981 tgaccgtgcg ctggtcagtg aaatgctgcg tatggataaa tacatcgaca tgctgatccc
     8041 gcgtggtggc gctggtttgc ataaactgtg ccgcgaacag tcgacgatcc cggtgatcac
     8101 aggtggtata ggcgtatgcc atatttatgt tgatgaaagt gtagagattg ctgaagcatt
     8161 aaaagtgatc gtcaacgcga aaactcagcg tccgagcaca tgtaatacgg tagaaacgtt
     8221 gctggtgaat aaaaacatcg ccgatagctt cctgcccgca ttaagcaaac aaatggagga
     8281 aagcggcgtg gcattacacg cagatgcagc tgcgctggcg cagttgcaga caggccccgc
     8341 gaaggtggtg gctgttaaag cggaagagta tgacgatgag tttctgtcat tagatttgaa
     8401 cgtcaaaatc gtcagtgatc ttgacgatgc catcgcccat attcgtgaac acggcacaca
     8461 acactccgat gcgatcctga cccgcgatat gcgcaacgcc cagcgttttg ttaacgaagt
     8521 ggattcgtcc gctgtttacg ttaacgcctc tacgcgtttt accgacggcg gccagtttgg
     8581 actaggtgcg gaagtggcgg taagcacaca aaaactccac gcgcgtggcc caatggggct
     8641 ggaagcactg accacttaca agtggatcgg tattggtgat tacaccattc gtgcgtaaat
     8701 aaaaccgggt gatgcaaaag tagccatttg attcacaagg ccattgacgc atcgcccggt
     8761 tagttttaac cttgtccacc gtgattcacg ttcgtgaaca tgtcctttca gggccgatat
     8821 agctcagttg gtagagcagc gcattcgtaa tgcgaaggtc gtaggttcga ctcctattat
     8881 cggcaccatt caaacatctc cccaagtcta ctaaagtctt tcaaaacccc ttataatccg
     8941 cgtattaaag ccccgttcgt cttttgacgt ctactaaagt ccctcaaaat ctacggtcag
     9001 atgggggtac ttatgggggt atttgctgtt cggtttagtg gaggtacccc caagtgaaac
     9061 tcaatgcccg tcaaatagac acagccaagc caaaagagaa ggcttacaag ctggccgatg
     9121 gtggcggttt gtatctcttg gtaaaaccta atggaggtaa atactggcga cttaaatatc
     9181 gtgtagctgg taaagagaag ctattggcac taggtgtgta tcctgaggtt actctagccg
     9241 atgctcgggc aaaacgtgaa gatgcgaaaa gaggtatcgc tggtggtatc gatccgatgg
     9301 aagcgaaacg agaggaaaag attgcccggg aaacgcagtt aaacaacacc ttcaaagata
     9361 ttgcccttga gtggcacagc agcaaattaa aaaaatggtc tgctggttat gcttcagaca
     9421 tcctcgaagc cttcaacaaa gatgtgttcc cttacattgg caaaaaacca atcgccgaaa
     9481 tcaaaccact tgaacttctg aatgtgctgc ggcgcatcga ggggcgcggt gctacagaaa
     9541 aagccaaaaa agtgaggcag cggtgcgggg aagttttccg ctatgcaata gtcactggtc
     9601 gcgcagagta taaccctgca ccagacctta ctagcgcgat gcaaggccat gaatctaatc
     9661 attacccttt ccttacagcc aaagaattac ctgatttttt caaggcattg tccagttact
     9721 caggaagcgc attggttgtt atggcggctc gtctactgat tatcaccggc ttgcgaactg
     9781 gcgaactacg tggcgcatta tgggatgaaa ttgatttcaa caaggctata tgggagatac
     9841 ccgcttcacg tatgaaaatg cggcggcctc atattgtgcc attgtctgag caggctcttt
     9901 cgcttattgg gaagattaga gaaataacag gcaattaccc tcttatgttt cccgggcgca
     9961 atgatccaag gaaaacaatg agtgaggcca gcataaacca agtgtttaag cgcattggct
    10021 acgctggacg tgtaactggt catgggttcc gacacactat gagcacgatt ttgcatgagc
    10081 agggctataa caccgcgtgg atagaaacgc agctcgctca cgttgataag aactcaattc
    10141 gtggcacata caaccatgcg caatatctgg atggaaggcg ggagatgctt caatggtatg
    10201 ccgactatat ggattctctc gagcatggcg gaaatgtggt gcatatggtg tttgaaaaac
    10261 acgcatgaaa caactggaca agtatacaga ggtttactaa agtatagggg agtagattga
    10321 taaaaactca cagaggattg ctatttaaca gttagccctg gagaagggca atcgctcttt
    10381 tacaaacgaa ggtgtattga gccttctacg tcgctgacaa agcggaatgt tctttaacag
    10441 gctttggcgc tgacaaagcg caattgttct ttaaaactac tggatgtcac gcctaatagc
    10501 gtgatcctgc cgaaatgcag ggtagctcta cccttgtgga gcaaacaaac caaaacaatc
    10561 tgactatctt ttataaaaga ccgcaatatg tttttcatat tgtggtcttt ggcgtattcg
    10621 acatttgacg caaataaaac tctcgccatt cgttgcagga gggggttatt gcgtcattag
    10681 tacaggatat taataaatgg ctaattacaa t
//
LOCUS       DBANUU010000572         8086 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.guided.175, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000572 DBANUU010000000
VERSION     DBANUU010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.guided.175"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            153..1961
                     /gene="frdA"
                     /locus_tag="ACIE68_005287"
     CDS             153..1961
                     /gene="frdA"
                     /locus_tag="ACIE68_005287"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFX5911389.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            1954..2688
                     /gene="frdB"
                     /locus_tag="ACIE68_005288"
     CDS             1954..2688
                     /gene="frdB"
                     /locus_tag="ACIE68_005288"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFX5911390.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            2699..3094
                     /gene="frdC"
                     /locus_tag="ACIE68_005289"
     CDS             2699..3094
                     /gene="frdC"
                     /locus_tag="ACIE68_005289"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFX5911391.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3105..3464
                     /gene="frdD"
                     /locus_tag="ACIE68_005290"
     CDS             3105..3464
                     /gene="frdD"
                     /locus_tag="ACIE68_005290"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFX5911392.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            3527..4660
                     /locus_tag="ACIE68_005291"
     CDS             3527..4660
                     /locus_tag="ACIE68_005291"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFX5911393.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            4749..5282
                     /gene="blc"
                     /locus_tag="ACIE68_005292"
     CDS             4749..5282
                     /gene="blc"
                     /locus_tag="ACIE68_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFX5911394.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(5279..5596)
                     /gene="sugE"
                     /locus_tag="ACIE68_005293"
     CDS             complement(5279..5596)
                     /gene="sugE"
                     /locus_tag="ACIE68_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFX5911395.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(5771..5917)
                     /gene="ecnB"
                     /locus_tag="ACIE68_005294"
     CDS             complement(5771..5917)
                     /gene="ecnB"
                     /locus_tag="ACIE68_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFX5911396.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(6028..6153)
                     /gene="ecnA"
                     /locus_tag="ACIE68_005295"
     CDS             complement(6028..6153)
                     /gene="ecnA"
                     /locus_tag="ACIE68_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFX5911397.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(6205..6771)
                     /gene="efp"
                     /locus_tag="ACIE68_005296"
     CDS             complement(6205..6771)
                     /gene="efp"
                     /locus_tag="ACIE68_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFX5911398.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            6813..7841
                     /gene="epmB"
                     /locus_tag="ACIE68_005297"
     CDS             6813..7841
                     /gene="epmB"
                     /locus_tag="ACIE68_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFX5911399.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2010 a         2085 c         2119 g         1872 t
ORIGIN      
        1 aaaaaatcga tctcgtcaaa tttcagactt atccatcaga ctatactgtt gtacctataa
       61 aggagcagtg gaatagcgtt cgcagaccgt aactttcagg tacttaccct gaagtacgtg
      121 gctgtgggat aaaaacaatc tggaggaatg tcgtgcaaac ctttcaagcc gatcttgcca
      181 ttgtaggcgc cggtggcgcg ggattacgtg ctgcaattgc tgccgcgcag gcaaatccga
      241 atgcaaaaat cgcactaatc tcaaaagtat acccgatgcg tagccatacc gttgctgcag
      301 aagggggctc cgccgctgtc gcgcaggatc atgacagctt cgaatatcac tttcacgata
      361 cagtagcggg tggcgactgg ttgtgtgagc aggatgtcgt ggattatttc gtccaccact
      421 gcccaaccga aatgacccaa ctggaactgt ggggatgccc atggagccgt cgcccggatg
      481 gtagcgtcaa cgtacgtcgc ttcggcggca tgaaaatcga gcgcacctgg ttcgccgccg
      541 ataagaccgg cttccatatg ctgcacacgc tgttccagac ctctctgcaa ttcccgcaga
      601 tccagcgttt tgacgaacat ttcgtgctgg atattctggt tgatgatggt catgttcgcg
      661 gcctggtagc aatgaacatg atggaaggca cgctggtgca gatccgtgct aacgcggtcg
      721 ttatggctac tggcggtgcg ggtcgcgttt atcgttacaa caccaacggc ggcatcgtta
      781 ccggtgacgg tatgggtatg gcgctaagcc acggcgttcc gctgcgtgac atggaattcg
      841 ttcagtatca cccaaccggt ctgccaggtt ccggtatcct gatgaccgaa ggctgccgcg
      901 gtgaaggcgg tattctggtc aacaaaaatg gctaccgtta tctgcaagat tacggcatgg
      961 gcccggaaac tccgctgggc gagccgaaaa acaaatatat ggaactgggt ccacgcgaca
     1021 aagtttctca ggccttctgg cacgaatggc gtaaaggcaa caccatctcc acgccgcgtg
     1081 gcgatgtggt ttatctcgac ctgcgtcacc tcggcgagaa aaaactgcat gaacgtctgc
     1141 cgttcatctg cgaactggcg aaagcgtacg ttggcgtcga tccggttaaa gaaccgattc
     1201 cggtacgtcc gaccgcacac tacaccatgg gcggtatcga aaccgatcag aactgtgaaa
     1261 cccgcattaa aggtctgttc gccgtgggtg aatgttcctc tgttggtctg cacggtgcaa
     1321 accgtctggg ctccaactcc ctggcggaac tggtggtctt cggccgtctg gccggtgaac
     1381 aagcgacaga gcgtgcagca actgccggta atggcaacga agcggcaatt gaagcgcagg
     1441 cagctggcgt tgaacaacgt ctgaaagatc tggttaacca ggatggcggc gaaaactggg
     1501 cgaagatccg cgacgaaatg ggcctggcaa tggaagaagg ttgcggtatc taccgtacgc
     1561 cggaactgat gcagaaaacc atcgacaagc tggcagagct gcaggaacgc ttcaagcgcg
     1621 tgcgcatcac cgacacctcc agcgtgttca acaccgacct gctctacacc attgaactgg
     1681 gccacggtct gaacgttgct gaatgtatgg cgcactccgc aatggcacgt aaagagtccc
     1741 gcggcgcaca ccagcgtctg gacgaaggtt gcaccgagcg tgacgacgtc aacttcctca
     1801 aacacaccct cgccttccgc gatgctgatg gcacgactcg cctggagtac agcgacgtga
     1861 agattactac gctgccgcca gctaaacgcg tttacggtgg cgaagcggat gcagccgata
     1921 aggcggaagc agccaataag aaggagaagg cgaatggctg agatgaaaaa cctgaaaatt
     1981 gaggtggtgc gctataaccc ggaagtcgat accgcaccgc atagcgcatt ctatgaagtg
     2041 ccttatgacg caactacctc attactggat gcgctgggct acatcaaaga caacctggca
     2101 ccggacctga gttaccgctg gtcctgccgt atggcgattt gtggttcctg cggcatgatg
     2161 gttaacaacg tgccaaaact ggcatgtaaa accttcctgc gtgattacac cgacggtatg
     2221 aaggttgaag cgttagctaa cttcccgatt gaacgcgatc tggtggtcga tatgacccac
     2281 ttcatcgaaa gtctggaagc gatcaaaccg tacatcatcg gcaactcccg caccgcggat
     2341 cagggtacta acatccagac cccggcgcag atggcaaagt atcaccagtt ctccggttgc
     2401 atcaactgtg gtctgtgcta cgccgcgtgc ccgcagtttg gcctgaaccc agagttcatc
     2461 ggtccggctg ccattacgct ggcgcatcgt tataacgaag atagccgcga ccacggtaag
     2521 aaggagcgta tggcgcagtt gaacagccag aacggcgtat ggagctgtac tttcgtgggc
     2581 tattgctccg aagtctgccc gaaacacgtc gatccggctg cggccattca gcagggcaaa
     2641 gtagaaagtt cgaaagactt tcttatcgcg accctgaaac cacgctaagg agtgcaacat
     2701 gacgactaaa cgtaaaccgt atgtacggcc aatgacgtcc acctggtgga aaaaattgcc
     2761 gttttatcgc ttttacatgc tgcgcgaagg cacggcggtt ccggctgtgt ggttcagcat
     2821 tgaactgatt ttcgggctgt ttgccctgaa aaatggcccg gaagcctggg cgggattcgt
     2881 cgacttttta caaaacccgg ttatcgtgat cattaacctg atcactctgg cggcagctct
     2941 gctgcacacc aaaacctggt ttgaactggc accgaaagcg gccaatatca ttgtaaaaga
     3001 cgaaaaaatg ggaccagagc caattatcaa aagtctctgg gcggtaactg tggttgccac
     3061 catcgtaatc ctgtttgttg ccctgtactg gtaaggagcc tgagatgatt aatccaaatc
     3121 caaagcgttc tgacgaaccg gtattctggg gcctcttcgg ggccggtggt atgtggagcg
     3181 caattattgc gccggtgatg atcctgctgg tgggtattct gctgccactg gggctgtttc
     3241 cgggtgatgc gctgagctac gagcgcgttc tggcgttcgc gcagagcttc attggtcgcg
     3301 tattcctgtt cctgatgatc gttctgccgc tgtggtgtgg tttacaccgt atgcaccacg
     3361 cgatgcacga tctgaaaatt cacgtgcctg cgggcaaatg ggttttctac ggtctggctg
     3421 ctatcctgac agttgtcacg ctgattggta tcgttacaat ctaacgtatc gccaatgtaa
     3481 atccggcccg cctatggcgg gccgttttgt atggaaacca gaccttatgt tcaaaacgac
     3541 gctctgcgcc ttattaatta ccgcctcttg ctccacattt gctgcccctc aacaaatcaa
     3601 cgatattgtg catcgcacaa ttaccccgct tatagagcaa caaaagatcc ccggtatggc
     3661 ggtggcggta atttatcagg gtaaacctta ttactttacc tggggctatg cggacatcgc
     3721 caaaaagcag cccgtcacac agcaaacgtt gtttgagtta ggttcggtca gcaaaacatt
     3781 tacgggcgtg cttggtggcg acgctattgc tcgaggggaa atcaagttaa gcgatcccgc
     3841 aacaaaatac tggcctgaac ttaccgctaa acagtggaat gggatcacac tattacatct
     3901 tgcgacctac accgctggcg gcctgccatt gcaggtgccg gatgaagtga aatcctcaag
     3961 cgacttgctg cgcttctatc aaaactggca gcctgcatgg gctccaggaa cacaacgtct
     4021 gtatgccaac tccagtatcg gtttgttcgg cgcactggct gtgaagccgt ctggtttgag
     4081 ttttgagcag gcgatgcaaa ctcgtgtctt ccagccactc aaactcaacc atacgtggat
     4141 taatgtacct cccccagaag aaaagaatta cgcctgggga tatcgcgaag gtaaggcagt
     4201 gcatgtttcg ccaggggcgt tagatgctga agcttatggt gtgaagtcga ccattgaaga
     4261 tatggcccgc tgggtacgaa gcaatatgaa tccccgtgat atcaacgaca aaacacttca
     4321 gcaagggata caactggcac aatctcgcta ctggcaaacc ggcgatatgt atcagggcct
     4381 gggctgggaa atgctggact ggccggtaaa tcctgacagc atcattaacg gcagtggcaa
     4441 taaaattgca ctggcagcac accctgtaaa agcgattacg cccccaactc ctgcagtacg
     4501 cgcatcatgg gtacataaaa caggggcaac cggcggattt ggtagctatg tcgcgtttat
     4561 tccagaaaaa gagctgggta tagtgatgct ggctaacaaa aactatccca atccagcgag
     4621 agtcgccgcc gcctggcaga ttctcaacgc tctacagtaa aatttcatcg ggtccgaatt
     4681 ttcggacctt ttctgcgctt ttccttgctg tcatctacac ttagaaaaaa accagtaagg
     4741 aaacatttat gcgcctgctc cctctcgttg ccgcagcgac agctgcattt ctggtcgttg
     4801 cctgcagttc tcctacgccg ccgcgtggcg taacagtagt gaataatttc gacgcaaaac
     4861 gttatctcgg tacctggtat gagattgccc gttttgatca ccgttttgaa cgtggactgg
     4921 aaaaagtcac cgcaacatac agtctgcgtg atgacggcgg cctgaatgtc atcaataaag
     4981 gctataaccc tgacagagaa atgtggcagc agagtgaagg gaaagcgtac tttaccggcg
     5041 caccaacacg cgctgcgctg aaagtgtcat tctttggtcc tttctatggc ggttataacg
     5101 tcattgcact tgatcgggaa taccgccatg cgctggtttg cggcccggac cgcgactacc
     5161 tgtggatact ctcccgcacg ccaaccattt ctgacgaagt gaaacaggag atgctggcag
     5221 tcgcgacccg ggaagggttt gatgtcagta agtttatttg ggtacagcag cccggtagtt
     5281 agtgagtgct gagtttcaga ccaataatcc ccaatacgat taacgccaga ctcgccaggc
     5341 gcatcgggtt agcggactca ccgagcagca caatgccggt gatggccgcg ccgactgcgc
     5401 caatacccgt ccacacggca taagccgtcc ctaccggtaa cgatttcatc gcccaggcaa
     5461 gtagcgccat actgactatc atcgccgtca cggtaataac actcggcgtc aaacggctaa
     5521 agccgtgggt atatttcagg ccaacggccc ataccacttc cagcagacca gcaataacta
     5581 agataatcca ggacatatca ggctccggag aattggggcc gtccccggtg aaaagaggcg
     5641 tttgcaggtc gtcctgcaaa gctgatggtt gaacggatat tttgctaggt ggcaggggaa
     5701 tttcaactat tacctttgtg actatcgcaa aaaaacaaac ggcacgacat aactgtcgta
     5761 ccgttattgc ttattgctgc gctttcgttg ctgcaccaga aatcgcgtta ccgccatcag
     5821 aaatgtcttc accaacgcca cgcgtggtgt tgcaggcagt taatactgtt gaaagcacca
     5881 gaacagaaaa gatcgctgca attgtcttct tcaccataac gtcttccttt taggtaatgt
     5941 tatttatgtt tgcctatagc aaattaataa tagacaacat cgccaaaaat gatggaaatc
     6001 tgatgatttt tcggaagaga agaaaaatta gctggcagcg cgggagatgg agttgccgag
     6061 atgtttgatg tcttcgccga aaccgcgagc ggtgttacag cccgtgagca gcgtgctggc
     6121 aagcaaaaca agaacgataa ggcgtttcat catctctgac ctgaataagt gatggtgcag
     6181 cctgcaggcc gcaccacaac cgcattactt cacgcgagag acgtattcac cagagcgggt
     6241 atccactttg atgacttcgc cgatttgtac gaacagcgga actttaacca cagcgccagt
     6301 agacagggta gccggtttgc cgccagtacc tgcggtatca cctttcaggc ccggatcggt
     6361 atcaacgatt tccagttcaa cgaagttcgg cggagtaacg gagatcggct gaccattcca
     6421 cagagttacg atacactctg cctgatccag cagccattta gcgttgtcgc caattgcttt
     6481 tgcatcagca gacagctgct cgaaagtttc gttgttcatg aagtgccaga actcaccgtc
     6541 gttgtacagg taagtcaggt tcatatcgac aacatcagcg ccttcagcgg aatcagtaga
     6601 tttgaaggtt ttttctacgc gagtaccggt cagcagacga cgcagtttaa cgcgagcaaa
     6661 tgcctggcct ttacccggtt ttacgaattc actcgcttca accgcgtaag gttcgccgtc
     6721 taacatgatt ttaagaccag cacgaaaatc gttgctatag tacgttgcca taaggccctc
     6781 tgaaatttgt taattggtag ctaagccaca aaatggcgca tattgtaacc ctaaataccc
     6841 catccagaga agattggtta acgcaacttg ccgatgttgt gaccgatcct gatgaacttc
     6901 tgcgtctttt gaatatagac gcggacgaaa aactgttggc cggacgcagc gccaaaaagc
     6961 tgtttgccct gcgtgtgccc cgctcattta tcgatcgcat ggagaaaggc aatccggacg
     7021 atcctctttt gcgtcaggta cttacctcgc aagatgagtt tgtcgtcgcg tccggattct
     7081 ccaccgaccc gctggaagaa cagcacagcg tagtgcctgg tttgttgcat aaataccaca
     7141 accgggcgct tttgctggtc aaaggcggct gcgcggtaaa ttgccgctat tgcttccgtc
     7201 gccacttccc ctatgccgaa aatcagggca acaagcgtaa ctggcagact gctcttgagt
     7261 atgttgctgc gcatccggaa ctggacgaga tgattttctc cggcggcgat ccgctgatgg
     7321 cgaaagatca cgagctggac tggttgctca cacaactgga agccatcccg catatcaaac
     7381 gtctgcggat tcacagccgt ctgccgattg tgatcccggc acgtatcacc gaggcgctgg
     7441 ttgaacgctt tgcccgttct acgctgcaaa tcttgctggt gaatcacatc aatcatgcca
     7501 atgaggtaga tgaaacgttc cgtcaggcga tggctaaatt gcgccgtgtc ggtgtcaccc
     7561 tgcttaacca gagcgttctg ttacgtggtg tgaacgataa cgcacaaacg ctggcaaacc
     7621 tgagtaatgc gttgttcgat gccggcgtaa tgccgtatta cctgcatgtg ctcgataaag
     7681 tacagggcgc ggcgcatttt atggtgagtg atgacgaagc acggcagatt atgcgtgagt
     7741 tgctgacact ggtgtcggga tatctggtgc cgaaactggc gcgagaaatt ggcggcgaac
     7801 ccagcaaaac gccgctggat ctccagctac gccagcagta actgattaaa cagggaagtc
     7861 aaacattaaa attgcctgat gcgctacgct tatcaggcct acatcgtgca ttgaaattga
     7921 atggatttgc aatgaactgt aggccggata aggcgtttac gccgcatccg gcaagagcaa
     7981 caaacgcaag tacagcaacc tgtcattaaa ctgggaaatt taatctttgc ccgttgcata
     8041 acctttaatt attcatatac attgctgttg ttactcttta tataac
//
LOCUS       DBANUU010000573         5508 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.guided.225, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000573 DBANUU010000000
VERSION     DBANUU010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.guided.225"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            163..1266
                     /locus_tag="ACIE68_005298"
     CDS             163..1266
                     /locus_tag="ACIE68_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFX5911400.1"
                     /translation="MSLLLNREHTNGQVTNASYAKVIETVLKSGVQADDRTGTGTLST
                     CYVPSYYMLTGGTVPLISGKAVNLKPLLVELEWYLKGTGNIQFLKDNGVKIWDAWADE
                     NGDLGPVYGKQWRRWEDTRIVSHSEYLSKIATFRERGYKVEGYLGISEDRVVLSREID
                     QLQRIVDTLRTNPTDRRIMLNAWNVGELEDMKLPPCHFVFSLWSRELDFETRLTMATD
                     IGLQHSRLGYESIYTKMLYDLEMDGSVTEAELDELGIPKRILNSCLVQRSVDTFVGMP
                     FNIAGYGILTHFLAKITGHMAGAFVHFGFDVHLYNNHMEGVCELMKRQAPEHSDPVVI
                     FPHEWSELDDFKWDEVLILGYDPLSWIKVPVAV"
     gene            1268..1681
                     /locus_tag="ACIE68_005299"
     CDS             1268..1681
                     /locus_tag="ACIE68_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911401.1"
                     /translation="MARGMYVLCEIEGVLANASHRKSVSDADAGQLIAGDELIFPTSR
                     MLRGFARSGAEVVLISSRSETLEAPTKRWLKDFGVDYDWLHLVPNGTSYEKHIKRTLA
                     EHKGDLLIAALVHDPRLRAALADSHHRPVIYEVSK"
     gene            1678..2154
                     /locus_tag="ACIE68_005300"
     CDS             1678..2154
                     /locus_tag="ACIE68_005300"
                     /inference="COORDINATES: protein motif:HMM:NF047324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFX5911402.1"
                     /translation="MKMIAAVGRNYEIGIANELPWRCSTDLKLFKRLTKNATVVMGRK
                     TMESLKRPLPERHNLVLTRSHGFVPNGFYPAGVDDVLRLPEPVWVIGGEQIYSLFMPH
                     VEEIWLSHIGVDVPNADAFFPASMMRNLGFVPVETAYTQRASEEEPGFSQIVYRRS"
     gene            2154..2798
                     /locus_tag="ACIE68_005301"
     CDS             2154..2798
                     /locus_tag="ACIE68_005301"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFX5911403.1"
                     /translation="MDYRIGITGAQGSGKTTLAKYIDKHYGIPYVDAGVGSLMSRLGV
                     RVGDSMPLYERLQIQMEIAKHIELLTRGVEGFVIDRTPADVMAYTLDLVGHTNEDRCI
                     ELALDIEKFCHKTAISNFNAIAGLRPGVALSERDYLRSQRASLDRLYVARIDALMCGE
                     LTKIHLHPQRGDLQTFVVSNRYRTVEARARSVMRMLDNAVEKIENRFCGRVTVH"
     gene            2860..3279
                     /locus_tag="ACIE68_005302"
     CDS             2860..3279
                     /locus_tag="ACIE68_005302"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911404.1"
                     /translation="MFSEMLLEDELDRKTTEALIRVADEHSRSLMSDREARLAIRAIF
                     ETAQGLVGTQVGEAINIAMSQFSEGSKKPLFPMHLMLAGGTVLYISVCLDSNQINILN
                     TVSGKWKDPIVCETSEETLKKAAQFVRSALLKGAKKL"
     gene            3289..3846
                     /locus_tag="ACIE68_005303"
     CDS             3289..3846
                     /locus_tag="ACIE68_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFX5911405.1"
                     /translation="MTTIVAGIDIESTGLDFLAGHKIIEIAITRYELETQRHIDSLEM
                     RFNPRRNIDPKAQAVHGISLEQLAAEPLLSNHASEIGAYMGACSVWVAHNGEAFDIPF
                     IRHEFSGYGVRLPEVPVIDTMLSGLWATEDGKRPRLEELAFSLGFIYDHAKAHSALYD
                     TNLMMQCFFKARNKYGFFKLPSEIV"
     gene            4005..4814
                     /locus_tag="ACIE68_005304"
     CDS             4005..4814
                     /locus_tag="ACIE68_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFX5911406.1"
                     /translation="MSSVENVMTNDDLDELAAMLQSLDEPVKKAAQVENTDDIDDLLL
                     GLDAGVAMSSDDVAEELFNEEKAGDFSSALNELELAHEPINVINAESGEAAENEPEQL
                     GIIEVEGCVEINDELKVQQSNDSNTNKKARTARGPRFALSDKDDAFFNKAGLEKDIFL
                     DAYDNAPVKAKDKILNLLNWFSGGPDISVYTVISMRHLLTEKKATSNSIKIALMSNPE
                     KPYPLNTASTQAGQMMAVFPATGIAVRDGGNLTLNEESPIVKKFVAEYTIG"
     gene            4998..>5508
                     /locus_tag="ACIE68_005305"
     CDS             4998..>5508
                     /locus_tag="ACIE68_005305"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFX5911407.1"
                     /translation="MIAAEKIKQRKRDNSLRDLWRTPDWLFSAIQRYLGVTFDVDVAC
                     NKDNAKLPNFIGVERDALKSEWGQPGTIAFLNPPYSKISPWIDAAIREQARGVTTVML
                     IPQSLDTKWYERATECANETIILSGGRVAFVEPDVNLGQVEVNINPGGSMLVVFRGFC
                     QDAGHSISKI"
BASE COUNT         1499 a         1154 c         1355 g         1500 t
ORIGIN      
        1 gttatctatc tatgtatatt gtgttggcgc gttaaattcc tgaaacaact attcgttttt
       61 ggtggcctat ggccaccatt tttttatcta tctggccttg ttctctcaat aaatgtaaac
      121 tcacgtaatg aataagtggt tacttatctt tgtgaggttt ttgtgtcact ccttttgaat
      181 cgtgagcata cgaacggtca ggtaacaaac gcatcgtatg caaaagttat tgagacggtg
      241 cttaaaagcg gcgtgcaggc tgatgatcgc acaggcactg gtactttaag cacctgctac
      301 gttccctctt actacatgct tactggtggg actgtgccgc ttatttctgg aaaggcggta
      361 aatcttaagc cactgcttgt cgaacttgag tggtatctga aaggcacggg caacatccaa
      421 tttctcaagg ataacggcgt taagatttgg gatgcatggg ccgatgagaa tggcgatttg
      481 gggccggttt acggtaagca gtggcgtcga tgggaagata cccgcatcgt gagccatagt
      541 gaatatctga gcaagatcgc tactttccgt gaacgcgggt acaaagtcga gggatacctg
      601 ggtatcagtg aagatcgcgt agtgctgtcc cgtgaaatcg atcagctaca gcgtattgtc
      661 gatacactgc gcacgaaccc taccgatcgt cgcatcatgc ttaacgcatg gaacgtaggc
      721 gagcttgagg atatgaaact gccaccttgc cactttgtct tctctttgtg gagtcgtgag
      781 ctggattttg aaacccgttt aacgatggca actgacattg gtcttcaaca cagtcgcctc
      841 ggttacgagt ctatctacac caagatgcta tacgatctgg agatggacgg cagtgttact
      901 gaagctgaac tggatgaact tggaatcccc aaacgcatcc tcaactcctg cctcgtacag
      961 cgtagcgtag acacttttgt tggtatgcca ttcaatattg ctggctatgg cattctcact
     1021 cattttctcg cgaagattac gggtcacatg gccggtgcat ttgtgcattt tggctttgac
     1081 gtgcatttgt acaacaacca catggaaggt gtgtgtgagc taatgaaacg acaggctcca
     1141 gagcattcag atccggtcgt tattttccct catgaatggt cagagttgga tgatttcaaa
     1201 tgggacgagg ttttaattct tggctatgac cctctatcgt ggatcaaggt tccagtggcg
     1261 gtgtgatatg gcaagaggta tgtatgtctt atgcgaaatt gaaggtgtgc tggcaaatgc
     1321 cagccatcgt aaatcagtat ctgacgcgga tgcaggccag ctcattgccg gtgatgaact
     1381 cattttcccc accagccgta tgttgcgtgg ttttgctcgc tcaggggctg aagtggtgct
     1441 tatcagtagc cgctctgaaa ctcttgaagc gccaactaaa cgatggctga aagattttgg
     1501 cgttgattat gactggcttc atctcgtacc gaatggcacc agttatgaga agcatattaa
     1561 gcgcacatta gcggagcata aaggcgatct gcttatcgct gcgctggtgc acgatcctcg
     1621 actccgtgcc gctttagccg actctcacca tcgaccggtc atctatgagg tgagcaaatg
     1681 aagatgatcg ctgctgttgg ccgtaactat gagatcggca tagcgaatga actcccctgg
     1741 cgttgttcta ccgatctgaa gctatttaag agactcacca aaaacgccac tgtcgttatg
     1801 gggcgtaaaa cgatggaaag tctcaaacgc cctcttccag agcgtcataa cctcgttttg
     1861 acgcgctctc atggctttgt accaaatgga ttctaccctg ctggtgtgga tgatgtgttg
     1921 cgattaccag agcctgtgtg ggtgattggc ggggaacaaa tttactcgct attcatgccg
     1981 catgttgaag agatttggct ctcccacatc ggcgttgatg tgccaaacgc cgatgcattc
     2041 ttcccggcaa gcatgatgcg taatttaggc tttgtgcctg ttgaaacagc ttatacccaa
     2101 cgagccagcg aggaagagcc tggcttttcg cagatcgtat acagaaggtc gtaatggatt
     2161 accggattgg gatcactggt gctcagggca gtgggaaaac aaccctggct aaatatatcg
     2221 acaaacatta cggaatccct tacgtggatg ctggtgtcgg aagtttgatg agtcgcctcg
     2281 gtgttcgagt aggtgattct atgcctctat atgagcggct tcagattcaa atggaaatag
     2341 caaagcatat agagctactt acgcgtggtg ttgaaggctt tgttatcgat cgcacacctg
     2401 ctgatgttat ggcctacacg ttggatttgg tcggccatac aaatgaagat cggtgtattg
     2461 agttagccct cgatatcgaa aagttttgcc acaaaactgc tatttcaaac tttaacgcca
     2521 ttgctggctt acgcccggga gtcgctctct cagagcgaga ttacttgcga tcacaacgag
     2581 catcattaga ccgtctgtat gtcgctcgta ttgatgcgtt gatgtgcggg gaactgacaa
     2641 aaattcacct gcatccgcaa aggggagatc tgcaaacctt cgtcgtttcc aaccgatatc
     2701 gcacggttga agcaagagcc agatcagtga tgagaatgct agataacgct gtagaaaaga
     2761 tagaaaaccg gttctgtggc cgagtgaccg ttcattagaa attgttcgcc tcttcgacat
     2821 tgcgacaata aaactctcaa aatgggttaa ggataaaaaa tgtttagtga aatgttgctt
     2881 gaagatgaac tggatcggaa aacaacagag gctttgattc gtgtagcgga cgaacattcc
     2941 cggtcgctta tgagcgatcg agaggctcgt ctggctattc gtgccatatt cgaaactgcg
     3001 caggggcttg ttggtacaca agtgggtgaa gccattaaca tcgccatgtc tcagttcagt
     3061 gaaggcagta aaaagcctct gtttcctatg catttgatgc tggctggtgg cacggtgctt
     3121 tatatctctg tttgtctgga tagcaaccaa atcaatattc tcaacactgt gtcaggtaag
     3181 tggaaagatc cgattgtctg tgaaaccagt gaagaaactt tgaaaaaagc ggctcaattt
     3241 gtacgtagcg cactacttaa gggcgctaag aagttgtaag gagttctgat gacaacgatt
     3301 gttgcaggca tcgatatcga gtctacggga ctggatttcc ttgctggtca taaaattatt
     3361 gaaatcgcaa ttacccgcta tgaactggag acacagagac atattgatag tctggagatg
     3421 cgttttaacc ctcgcagaaa catagatccg aaagctcaag ccgttcatgg catttcattg
     3481 gaacagctcg cagctgaacc tttgttgtca aatcatgcca gcgaaattgg cgcttatatg
     3541 ggggcatgta gtgtgtgggt tgctcataac ggcgaagcat ttgatatacc atttattcga
     3601 cacgagtttt cagggtatgg agtaagactg ccagaagttc ctgttataga tactatgtta
     3661 tcgggattgt gggccacaga agacggtaaa cgtccccgcc ttgaagagtt ggccttctct
     3721 cttggcttta tatacgatca tgccaaagca catagtgcct tatatgacac aaacttaatg
     3781 atgcaatgct tctttaaggc acgtaataag tacggatttt ttaaattacc ctctgaaatt
     3841 gtgtaaaaca aaagcctact ttaaaaagtt taaagtaggc tttcttttaa agaacagtcg
     3901 cctttcaatc atttcctgcc tgtatttaat acttttccgc ctgataggtt tagtcaaaat
     3961 gtagccatcg aaacgcaaat gtaaccaaac agaaggagac ttacatgagt tcggttgaaa
     4021 atgtaatgac aaatgatgat ctggacgagc tggcagccat gttgcaatca cttgatgaac
     4081 cagtaaaaaa agctgcacag gttgaaaata ctgatgatat tgacgatctg ctgctcggcc
     4141 tagatgctgg cgtagccatg agttctgatg atgttgccga agaactgttc aatgaagaaa
     4201 aagcaggtga tttcagctca gctttaaatg agttggagtt agcgcatgag cctataaacg
     4261 taattaacgc tgaaagtggt gaagctgccg aaaacgaacc agaacagttg gggatcatcg
     4321 aggttgaagg gtgtgttgag attaatgatg aattaaaagt tcaacagtca aatgatagca
     4381 acacaaataa aaaagcgcgt actgcaagag gtcctcgttt tgctctaagt gataaagatg
     4441 atgcgttttt caataaagcg ggtttagaga aagatatttt tttagacgct tacgataacg
     4501 cgcctgtcaa agcaaaggat aagatattaa accttcttaa ttggtttagc ggaggtccag
     4561 atattagtgt ttacacggta atttccatga gacaccttct cacagaaaag aaggctacaa
     4621 gtaatagtat taagattgct ttaatgagca atccagaaaa accgtatccg cttaacactg
     4681 cgtcaactca ggctgggcaa atgatggctg tatttccagc gacaggaatt gccgttagag
     4741 acggtggaaa tctaacattg aacgaagaat caccgatcgt taagaagttt gtcgcggagt
     4801 acactattgg atgacgttcc cctactgaaa ataaagccca tagagagctt tatagtgctg
     4861 ggtaagccaa tcacataccc agcaccacaa aaacgcgcca gagagcttct cgtttgcatt
     4921 tctggcgcgt tttatttgat tgccagacat aaaatcaaat gcaaaaatag gtatttactt
     4981 acctatcgag aaagaagatg attgcagccg aaaaaatcaa acagcgaaag cgcgacaact
     5041 ctcttcgtga cctctggaga acacctgact ggctgttttc tgccattcaa cgttatcttg
     5101 gagtgacatt tgatgttgac gttgcctgca acaaggacaa tgcaaagctg cctaatttca
     5161 taggcgttga gcgtgatgct ttgaaatctg aatggggaca gccaggtaca attgccttcc
     5221 tcaatccacc ctactccaaa atctccccct ggattgatgc ggctatacgt gagcaggctc
     5281 gcggagttac aacagtgatg ctaattcctc aatccctaga tacaaagtgg tatgagcgtg
     5341 caacagagtg tgcgaatgag acgattattc tgtctggtgg ccgcgtagcg tttgtcgagc
     5401 ctgacgtcaa tctgggtcag gtagaagtaa acatcaaccc aggtggcagt atgctcgttg
     5461 tttttcgagg attctgtcag gacgctgggc actctataag caagatcc
//
LOCUS       DBANUU010000574         4554 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.guided.245, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000574 DBANUU010000000
VERSION     DBANUU010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.guided.245"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            complement(<1..166)
                     /locus_tag="ACIE68_005306"
     CDS             complement(<1..166)
                     /locus_tag="ACIE68_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFX5911408.1"
                     /translation="MILDASYTLLVACIALLIGMFVVKFTPFLQKNHIPEAVVGGFIV
                     AIVLLIIDKTS"
     gene            610..930
                     /locus_tag="ACIE68_005307"
     CDS             610..930
                     /locus_tag="ACIE68_005307"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HFX5911409.1"
                     /translation="MIAVIFEVQIQPDQQTRYLTLAEELRPLLSHVAGFISIERFQSL
                     ATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGRESIFDFYKISIAHITREYSFKKD
                     KDNV"
     gene            923..1309
                     /locus_tag="ACIE68_005308"
     CDS             923..1309
                     /locus_tag="ACIE68_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HFX5911410.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            1317..2003
                     /locus_tag="ACIE68_005309"
     CDS             1317..2003
                     /locus_tag="ACIE68_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFX5911411.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1981..2604)
                     /gene="tetR(B)"
                     /locus_tag="ACIE68_005310"
     CDS             complement(1981..2604)
                     /gene="tetR(B)"
                     /locus_tag="ACIE68_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFX5911412.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2686..3891
                     /gene="tet(B)"
                     /locus_tag="ACIE68_005311"
     CDS             2686..3891
                     /gene="tet(B)"
                     /locus_tag="ACIE68_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFX5911413.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(4004..>4554)
                     /locus_tag="ACIE68_005312"
     CDS             complement(4004..>4554)
                     /locus_tag="ACIE68_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFX5911414.1"
                     /translation="QSLVNSARILFVEKGYQAVSIDEISGKALVTKGAFYHHFKNKKQ
                     LLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIIDNNKNLIPIQEVMPII
                     GWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLNGWFMHIAIHAKNLKE
                     LADKKGQFIAIYRGFLLSLKDK"
BASE COUNT         1326 a          804 c          948 g         1476 t
ORIGIN      
        1 ctgatgtttt atcaataatt aacagaacaa ttgcaacaat aaagccacca acgacggctt
       61 ctggtatgtg gtttttttgt aggaacgggg taaattttac gacaaacatt cctatgagta
      121 gcgcgataca tgcgactaat aatgtataac tggcatctag gatcattttt ttacctctat
      181 tagtttacca gaattttcac tataacaatt taacaatagt gaaaaatgaa gttactttgg
      241 ttaactaatc tttaacaatg agttttatta gggtatccat aacttttagt taatagggtc
      301 ataattttag tgtgatctta atcatgaaat attaaaaaga ttaaaataac aacaatggta
      361 tttaatgatg ggtaaaccta aataccatgc gggatccgca ctattttcgg tgatagcaaa
      421 aaataaaaaa gtataaataa taaacaaaat tataagagaa ggagaacatg ataagcgcag
      481 gccgagcaat ttaaagaagt gtgatataca acataaaaca gcagcatgac ctttctatat
      541 ttcgttgctt atcgaagtgt taattttcgg gtggattact ctgtttgttg attaataacg
      601 gagaaaaata tgattgctgt aatatttgag gtgcaaatac aacccgacca acaaactcgc
      661 tatttgactt tagctgagga gttaagacca ctattaagtc atgtagctgg ttttatttca
      721 attgaacgtt ttcaaagtct agctacagaa ggaaaaatgt tatcgctatc ttggtgggaa
      781 aacgaatacg cagttctgca atggaaaaat catgttttac atgcgaaagc tcaacaagaa
      841 gggcgagagt caatatttga tttttacaaa attagtattg ctcatattac tcgcgaatat
      901 tcatttaaaa aggacaagga taatgtttga tgttcacgtt gttttagata atcaaatagg
      961 acaattagca ttactaggaa aaacattagg taataaaggt attggattgg aagggggagg
     1021 gatatttacg gttggtgatg aatgccatgc tcattttctt gttgaacaag gaaaggaagc
     1081 taaaatagcg ctagagcaag ctggactgtt agtacttgcg atccggacac cattaattcg
     1141 taagttaaaa caggaaaaac cgggggaact tggcgaaata gcacgagtat tggcggagaa
     1201 taacattaat attttagtgc aatacagtga ccatgctaac caactgatat taataacgga
     1261 caatgatagt atggctgcat ctgttacgct cccttgggca ataaagtgaa cttgcgatgg
     1321 ctaatttaat acgaaaagag gttacctttg agtcctcaat agccgcgata ggggcggcta
     1381 tgtctgacat ttcacgagtt aaaatactca gtgctttgat ggatgggcga gcttggacgg
     1441 ccactgagct aagttctgtg gcgaatatat cagcttcaac ggcgagcagt catttatcta
     1501 aattattaga ttgccagcta atcacagtag tagctcaagg caagcatcgt tattttcggc
     1561 tagcaggaaa agatattgct gaattgatgg aaagtatgat ggggatctcc ttaaaccatg
     1621 gcgtacatgc caaagtttcc acgccagtgc atttacgaaa agcacgtact tgctatgatc
     1681 atttagctgg cgaagttgcc gttaagatct atgattccct ttgtcaacag caatggatca
     1741 ctgaaaatgg ttcaatgatc acattaagtg gtattcaata ttttcatgaa atgggaattg
     1801 acgttccttc caaacattca cgtaaaatct gttgtgcgtg tttagattgg agtgaacgcc
     1861 gtttccattt aggtgggtac gttggagccg cattattttc gctttatgaa tctaaagggt
     1921 ggttaactcg acatcttggt taccgtgaag ttaccatcac ggaaaaaggt tatgctgctt
     1981 ttaagaccca ctttcacatt taagttgttt ttctaatccg catatgatca attcaaggcc
     2041 gaataagaag gctggctctg caccttggtg atcaaataat tcgatagctt gtcgtaataa
     2101 tggcggcata ctatcagtag taggtgtttc cctttcttct ttagcgactt gatgctcttg
     2161 atcttccaat acgcaaccta aagtaaaatg ccccacagcg ctgagtgcat ataatgcatt
     2221 ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga ttttcgagag tttcatactg
     2281 tttttctgta ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac ttagtaaagc
     2341 acatctaaaa cttttagcgt tattacgtaa aaaatcttgc cagctttccc cttctaaagg
     2401 gcaaaagtga gtatggtgcc tatctaacat ctcaatggct aaggcgtcga gcaaagcccg
     2461 cttatttttt acatgccaat acaatgtagg ctgctctaca cctagcttct gggcgagttt
     2521 acgggttgtt aaaccttcga ttccgacctc attaagcagc tctaatgcgc tgttaatcac
     2581 tttactttta tctaatctag acatcattaa ttcctaattt ttgttgacac tctatcattg
     2641 atagagttat tttaccactc cctatcagtg atagagaaaa gtgaaatgaa tagttcgaca
     2701 aagatcgcat tggtaattac gttactcgat gccatgggga ttggccttat catgccagtc
     2761 ttgccaacgt tattacgtga atttattgct tcggaagata tcgctaacca ctttggcgta
     2821 ttgcttgcac tttatgcgtt aatgcaggtt atctttgctc cttggcttgg aaaaatgtct
     2881 gaccgatttg gtcggcgccc agtgctgttg ttgtcattaa taggcgcatc gctggattac
     2941 ttattgctgg ctttttcaag tgcgctttgg atgctgtatt taggccgttt gctttcaggg
     3001 atcacaggag ctactggggc tgtcgcggca tcggtcattg ccgataccac ctcagcttct
     3061 caacgcgtga agtggttcgg ttggttaggg gcaagttttg ggcttggttt aatagcgggg
     3121 cctattattg gtggttttgc aggagagatt tcaccgcata gtcccttttt tatcgctgcg
     3181 ttgctaaata ttgtcgcttt ccttgtggtt atgttttggt tccgtgaaac caaaaataca
     3241 cgtgataata cagataccga agtaggggtt gagacgcaat cgaattcggt atacatcact
     3301 ttatttaaaa cgatgcccat tttgttgatt atttattttt cagcgcaatt gataggccaa
     3361 attcccgcaa cggtgtgggt gctatttacc gaaaatcgtt ttggatggaa tagcatgatg
     3421 gttggctttt cattagcggg tcttggtctt ttacactcag tattccaagc ctttgtggca
     3481 ggaagaatag ccactaaatg gggcgaaaaa acggcagtac tgctcggatt tattgcagat
     3541 agtagtgcat ttgccttttt agcgtttata tctgaaggtt ggttagtttt ccctgtttta
     3601 attttattgg ctggtggtgg gatcgcttta cctgcattac agggagtgat gtctatccaa
     3661 acaaagagtc atcagcaagg tgctttacag ggattattgg tgagccttac caatgcaacc
     3721 ggtgttattg gcccattact gtttgctgtt atttataatc attcactacc aatttgggat
     3781 ggctggattt ggattattgg tttagcgttt tactgtatta ttatcctgct atcgatgacc
     3841 ttcatgttaa cccctcaagc tcaggggagt aaacaggaga caagtgctta gttatttcgt
     3901 caccaaatga tgttattccg cgaaatataa tgaccctctt gataacccaa gagggcattt
     3961 tttacgataa agaagattta gcttcaaata aaacctatct attttattta tctttcaagc
     4021 tcaataaaaa gccgcggtaa atagcaataa attggccttt tttatcggca agctctttta
     4081 ggtttttcgc atgtattgcg atatgcataa accagccatt gagtaagttt ttaagcacat
     4141 catcatcata agctttaagt tggttctctt ggatcaattt gctgacaatg gcgtttacct
     4201 taccagtaat gtattcaagg ctaatttttt caagttcatt ccaaccaatg ataggcatca
     4261 cttcttggat agggataagg tttttattat tatcaataat ataatcaaga taatgttcaa
     4321 atatactttc taaggcagac caaccatttg ttaaatcagt ttttgttgtg atgtaggcat
     4381 caatcataat taattgctgc ttataacagg cactgagtaa ttgtttttta tttttaaagt
     4441 gatgataaaa ggcacctttg gtcaccaacg cttttcccga gatctcatct attgaaacag
     4501 cttgatagcc tttttcaaca aacaatattc gtgctgagtt aaccagtgat tgat
//
LOCUS       DBANUU010000575         3402 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.guided.279, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000575 DBANUU010000000
VERSION     DBANUU010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.guided.279"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            157..972
                     /locus_tag="ACIE68_005313"
     CDS             157..972
                     /locus_tag="ACIE68_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HFX5911415.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(1115..2137)
                     /locus_tag="ACIE68_005314"
     CDS             complement(1115..2137)
                     /locus_tag="ACIE68_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HFX5911416.1"
                     /translation="MKKIDISGSPFEIGLRLGELGREAWHQKLMLTSLWQTVMTLQAS
                     PETRLMRAEVQRHYPHIWQELEGLAQGLEATVDQVFAWNCRGDLVRSTSDGCTTVAGV
                     NAAGERIIAHNEDGFPQLRDECLLATITPSRGLSFTSFAYPGSICGHTFAVNEKGVVN
                     TVNNIRAVHRPQGLPRQILARASLNANTLDEAITILTGQPRSGAFHHTLGQLGDTRLF
                     SVEATGQGCSVLPLTTVTGHANHLVHAALAGVEQIVTDSSASRQLRLVQWRETQPPFD
                     AAAAKAILSDTHDAELPIYRLAADDPDEENTLATAVFTLDANHVRWQIFDINRDDAKF
                     QGEVRG"
     gene            complement(2157..2879)
                     /gene="glnQ"
                     /locus_tag="ACIE68_005315"
     CDS             complement(2157..2879)
                     /gene="glnQ"
                     /locus_tag="ACIE68_005315"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFX5911417.1"
                     /translation="MVEFSAVAKHFGTTQVLYDINLKIDAGEVVVIIGPSGSGKSTLL
                     RCINKLEEISSGTLLVAGMHLTDPHVNECEIRREAGMVFQQFHLFPHLTALENVMFGP
                     IRVRKMSQAAAREQALALLERVGLGERAEHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMRSLADEGMTMVIVTHEIGFARDVASRLIFIDGGTIAEDG
                     PPDILLNASSNPRLKEFLQHVS"
     gene            complement(2882..>3402)
                     /locus_tag="ACIE68_005316"
     CDS             complement(2882..>3402)
                     /locus_tag="ACIE68_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308915.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFX5911418.1"
                     /translation="LQLSKSSIYSTKPELIRGTPIMVQVMFIYFALPMILPVRIDPIT
                     AAMVTIIINSGAYIAEITRGAILSINRGFKEASLAMGLSQRATLWHVIMPLALRRMIP
                     ALGNQWIISIKDTSLFIVIGVAELTRQGQEIIAGNFRALEVWTAVALIYLAVTLCLSF
                     LLKQLEKRIHIL"
BASE COUNT          835 a          896 c          855 g          816 t
ORIGIN      
        1 cagggtcgtt cgcacgaaat cttatttatt caacaaagcc atggcgggaa ccacgttgtg
       61 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
      121 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
      181 tcttgctcga ggccgcgatt aaattccaac ctggatgctg atttatatgg gtataaatgg
      241 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat
      301 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
      361 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
      421 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag
      481 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
      541 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
      601 ctcgctcagg cgcaatcacg aatgaacaac ggtttggttg atgcgagtga ttttgatgac
      661 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc
      721 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
      781 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
      841 cttgccatcc tatggaactg cctcggtgaa ttttctcctt cattacagaa acggcttttt
      901 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
      961 gagtttttct gatagttagt ctttgtagag acacaaacct gaaattccgg acaccgcgct
     1021 tcaggacatc gctcaagacg cctatatcgg gcagtatccg gacggtacta tcgaacgccg
     1081 ttaccctgcc ctttatgact gacgaccggc tatctcaccc acgcacttct ccctgaaatt
     1141 tagcatcgtc gcggttaatg tcgaaaattt gccacctgac gtggttggca tcgagggtga
     1201 aaactgccgt ggcgagggtg ttctcctcat ccggatcgtc ggcagccagc cggtaaattg
     1261 gcagttcggc gtcatgggta tctgagagaa tggcttttgc cgccgcggcg tcaaacggag
     1321 gttgcgtttc ccgccactgc acgaggcgta actgccgcga cgctgaactg tcggtaacga
     1381 tctgctcaac gccagccagt gccgcatgca ccagatggtt ggcatgcccg gttaccgtcg
     1441 tcagggggag aacggaacag ccttgaccgg tggcctcaac gctgaacagc cgcgtatcgc
     1501 ccagctgccc gagcgtatgg tgaaacgccc cggatcgcgg ctggccggta agtatagtga
     1561 tggcctcgtc gagggtgttc gcattcagcg aggcgcgagc caggatctga cgcggtaatc
     1621 cctgcggacg atgcactgca cggatgttat tcaccgtgtt aacgacacct ttttcattca
     1681 ccgcaaaggt gtggccgcaa atcgaccccg gataggcaaa actcgtaaag ctcagaccgc
     1741 gactcggggt aatggtagcc agcaagcatt catcccgtaa ctgaggaaaa ccgtcttcat
     1801 tgtgcgcgat aatccgctcg cctgcggcgt ttaccccggc caccgtcgta cagccatccg
     1861 acgttgagcg caccagatcg ccacggcagt tccaggcaaa gacctgatca acagtagcct
     1921 ccagcccctg cgccaacccc tccagctcct gccagatgtg cggataatgc cgctgcacct
     1981 cagcgcgcat taaccgggtc tcaggggatg cctgcagggt catcaccgtt tgccacaatg
     2041 acgtgagcat cagtttctga tgccatgctt cacggccaag ctcgccaagg cgcaggccaa
     2101 tctcaaacgg ggaaccactg atgtcgattt tcttcatgtg atggattcac ttatgttcag
     2161 gaaacgtgct gtaaaaattc tttcaggcgc ggattgctac tggcattcag caggatatca
     2221 ggtgggccat cttcagcaat ggttccacca tcgataaaaa tcaatcgaga ggccacatcg
     2281 cgtgcaaagc caatctcgtg cgtcactata accatcgtca tcccttcatc cgccagtgaa
     2341 cgcatcactt ttaatacttc atggcgcaac tccggatcga gcgcagaggt tggctcatca
     2401 aacagcatca ttttcggttt caccgccagc gcgcgggcga tagccacgcg ctgctgctgg
     2461 ccgcctgaaa gctccgacgg atagtgctcg gcccgttcgc ccaggccgac acgttccagc
     2521 agcgccagcg cctgttcgcg ggctgccgct tgactcattt tgcgtacgcg aataggccca
     2581 aacatcacat tttccagcgc cgtcagatgc ggaaacagat gaaactgctg gaataccatc
     2641 cccgcttcgc ggcggatttc gcactcgttt acgtgaggat cggtcaggtg cattccggca
     2701 accaacaaag tgccagagga gatctcttcc agcttgttga tacaacgcag cagcgtcgat
     2761 ttacctgaac ctgaaggacc gataatcacg accacttcac cggcatcaat tttcagattg
     2821 atatcgtaca gcacctgggt ggtgccgaaa tgtttagcga ccgcgctaaa ttcaaccatg
     2881 ctcataaaat atggatcctt ttctccagtt gcttcagcag aaagctcagg cacagggtga
     2941 cggcaagata aatcagggct accgctgtcc agacctcaag ggcacggaag ttcccggcga
     3001 tgatctcctg cccctgccgc gttaattccg cgacaccgat aacaatgaac agcgaggtgt
     3061 ctttaatgct gataatccac tggttgccca acgccgggat catgcggcgc agggccagcg
     3121 gcataatcac atgccatagc gttgcgcgct gcgataatcc catcgccaga ctggcttctt
     3181 taaatcctct attgatggac aaaattgcgc cgcgcgtaat ttccgcaata tacgcgccgg
     3241 agttaataat aatggtgacc atcgcggccg tgatagggtc gatacgaaca ggcagaatca
     3301 ttggcaaggc aaaatagata aacatcacct gtaccataat tggcgtaccg cgaatcaatt
     3361 cgggctttgt tgaataaatc gaacttttgc tgagttgaag ga
//
LOCUS       DBANUU010000576         1785 bp    DNA     linear   BCT 02-NOV-2024
DEFINITION  TPA_asm: Escherichia coli isolate ESBL20180399
            SAMEA115626925-rid22814933.guided.343, whole genome shotgun
            sequence.
ACCESSION   DBANUU010000576 DBANUU010000000
VERSION     DBANUU010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115626925
            Sequence Read Archive: ERR13115909
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002317706.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002317706.1
            Genome Coverage       :: 112x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/20/2024 06:38:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 76 of 195
            Pseudo Genes (incomplete)         :: 126 of 195
            Pseudo Genes (internal stop)      :: 41 of 195
            Pseudo Genes (multiple problems)  :: 42 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115626925-rid22814933.guided.343"
                     /isolate="ESBL20180399"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
     gene            167..1042
                     /locus_tag="ACIE68_005317"
     CDS             167..1042
                     /locus_tag="ACIE68_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase
                     CTX-M-1"
                     /protein_id="HFX5911419.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAVAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVDGTMSLAELSAAALQYSDNVAMNKLISHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGDYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTNGL"
     gene            complement(1089..1421)
                     /locus_tag="ACIE68_005318"
     CDS             complement(1089..1421)
                     /locus_tag="ACIE68_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HFX5911420.1"
                     /translation="MRPHRHPHTFELLLPLRGRFVVLNFDDRGTVTHRAILGETCTVL
                     EMAAGTWHAVLSLDTGGIIFEVKHGGYQPVAADDYAHWAPAEGEPGTTELMAWYAQAQ
                     VGDSAFAV"
     gene            complement(1435..1686)
                     /locus_tag="ACIE68_005319"
                     /pseudo
     CDS             complement(1435..1686)
                     /locus_tag="ACIE68_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
BASE COUNT          453 a          457 c          471 g          404 t
ORIGIN      
        1 taatgttaca atgtgtgaga agcagtctaa attcttcgtg aaatagtgat ttttgaagct
       61 aataaaaaac acacgtggaa tttaggttag actataaata gaaaaaggcg ttttgacaga
      121 ctattcatgt tgttgttaat tcgtctcttc cagaataagg aatcccatgg ttaaaaaatc
      181 actgcgtcag ttcacgctga tggcgacggc aaccgtcacg ctgttgttag gaagtgtgcc
      241 gctgtatgcg caaacggcgg acgtacagca aaaacttgcc gaattagagc ggcagtcggg
      301 aggaagactg ggtgtggcat tgattaacac agcagataat tcgcaaatac tttatcgtgc
      361 tgatgagcgc tttgcgatgt gcagcaccag taaagtgatg gccgtggccg cggtgctgaa
      421 gaaaagtgaa agcgaaccga atctgttaaa tcagcgagtt gagatcaaaa aatctgactt
      481 ggttaactat aatccgattg cggaaaagca cgtcgatggg acgatgtcac tggctgagct
      541 tagcgcggcc gcgctacagt acagcgataa cgtggcgatg aataagctga tttctcacgt
      601 tggcggcccg gctagcgtca ccgcgttcgc ccgacagctg ggagacgaaa cgttccgtct
      661 cgaccgtacc gagccgacgt taaacaccgc cattccgggc gatccgcgtg ataccacttc
      721 acctcgggca atggcgcaaa ctctgcgtaa tctgacgctg ggtaaagcat tgggtgacag
      781 ccaacgggcg cagctggtga catggatgaa aggcaatacc accggtgcag cgagcattca
      841 ggctggactg cctgcttcct gggttgtggg ggataaaacc ggcagcggtg actatggcac
      901 caccaacgat atcgcggtga tctggccaaa agatcgtgcg ccgctgattc tggtcactta
      961 cttcacccag cctcaaccta aggcagaaag ccgtcgcgat gtattagcgt cggcggctaa
     1021 aatcgtcacc aacggtttgt aatagcggaa acggaatggg gaaactcatt ccgtttttgt
     1081 ttatcgcctt agacggcaaa agcgctgtcg cccacctgcg cttgcgcata ccaggccata
     1141 agctccgtgg ttcctggttc tccttccgct ggcgcccagt gcgcatagtc atcggcagcc
     1201 acgggttgat agccaccgtg ttttacttca aaaattatgc caccggtatc cagcgacagc
     1261 acggcatgcc aggttcctgc ggccatctcc agcaccgtac aggtttcccc caatatcgcc
     1321 cgatgggtga cggtaccccg atcgtcaaaa ttcagcacca cgaaacgacc ccttaatggc
     1381 aacagtagtt cgaaggtgtg agggtgtcgg tgcgggcgca cgtaggttcc tggttataat
     1441 aatggcctca cctgccacca ttcctgacgg cccccagtag ataaaccaca ttgcacatcc
     1501 ccaggaaatg ccccataagc cccccgtcag caccgtgacc tgccagaacg gcataaaggg
     1561 taatggcgga agccggatac ccagccgcca gagaatacgc agaagaggag gggcataatt
     1621 gctccgccac atctttttgc tgtccatcag ggcaatggcc cgggcttttt tctgctcaaa
     1681 ggtcacagga gcacctccgt tccatgatgt aaacgtgaca cagcttattg ttattgatta
     1741 ttattatcat gctgtggtta tgttgtcatg catcagtaca ctgac
//
